LOCUS       DAORYU010000001        31240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_1216_length_31240_cov_4.089338, whole genome shotgun sequence.
ACCESSION   DAORYU010000001 DAORYU010000000
VERSION     DAORYU010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 31240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31240
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1216_length_31240_cov_4.089338"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1014
                     /locus_tag="PLW07_00005"
     CDS             <1..1014
                     /locus_tag="PLW07_00005"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007956427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOL48939.1"
                     /translation="KKYNVAVIGAGLVGKKMVETLIERNFPFADLKVLATREREEIIA
                     GRRFQVEKTSEDSFTGIDFAFFAGTEGEKGASIEFGWKAVEKGAIVIDNGGDFRMHPD
                     VPLVIPEVNAEHLKKHKGFIANPNCSTIIMLMAVAPLNARYRVKRIVVSTYQAVSGTG
                     SKALEELENQSHQYEKNQEMTVRVYPHRILFNVIPHIGNLSEQFAGYYSEEVKMLLET
                     RKILDRPDMLVSATCVRVPVFNGHSEAITIQFETDPDVDECREILSSAKGVKLLDDPG
                     NSQYPIPIEVSGKDDVYVGRIRKNTALDHAIDMWVVGDNIKKGAAQNAVQIAEAMIEM
                     NLI"
     gene            complement(1084..1581)
                     /locus_tag="PLW07_00010"
     CDS             complement(1084..1581)
                     /locus_tag="PLW07_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL48940.1"
                     /translation="MKITFFRLFLLSLYFFVNCLISGTDIRIPFELWPKVEEKISDVT
                     ILVAKTEQLSHQGFCGATSEQFQTTVIWFPDIKEETVFVNTNPGYGTVNQDLQVVFLN
                     KNWLVLDIKTMEKHTGTAVAPRGTFSALEGIPDVIRKLGFKKNKPSPFRIQKIGNRYF
                     GIKHQ"
     gene            complement(1578..2090)
                     /locus_tag="PLW07_00015"
     CDS             complement(1578..2090)
                     /locus_tag="PLW07_00015"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HOL48941.1"
                     /translation="MKELYDAIRTIPDFPKRGVLFRDITPLIKNEDLFARSIELFAKQ
                     VPDGINKIVAIESRGFIFGGALACKLNKGFVPIRKLGKLPCKTIKHTYALEYGEDTIE
                     IPADAFEKNETVVLIDDVLATGGTAGAACSLIERAGAKVEKILFLIELKDLNGREKIK
                     GYDIYSMLTL"
     gene            complement(2074..2898)
                     /locus_tag="PLW07_00020"
     CDS             complement(2074..2898)
                     /locus_tag="PLW07_00020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOL48942.1"
                     /translation="MKNKISTGKILKKPSRIEELELLKKIKQGDRDAQEKFMKMNQGL
                     VVSIARRYAFSNDILEDLISEGNIGLIEAIKKFDPKKHARFGTYAYFWVRRYIMRAMS
                     TSSFKVPEKIKKLKTKYKNVIEKVKLEENRCPTNTEMASLLKLDVPTFLKYKPYFEST
                     KISPFFQDSENEDYDLFDITNFTSSKKQWDSILVDKDILNKLFRRLQKKIKSGSIDIW
                     LKALKLHYGIDDGIPRSYKEIAEQMGFSRQRVHQIIKTCLKHLHNEIKEMKNERII"
     gene            complement(2903..5515)
                     /gene="alaS"
                     /locus_tag="PLW07_00025"
     CDS             complement(2903..5515)
                     /gene="alaS"
                     /locus_tag="PLW07_00025"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOL48943.1"
                     /translation="MKSNEIRKKFLEFFEKKSHTVVPSSSLIPFEDPTLLFTSAGMVQ
                     FKKFWATEVPLPYSRATSCQKCLRAGGKDSDLEKIGLSGRHHTFFEMLGNFSFGDYFK
                     EEAIHWAWEFVTLILKISKEKLWASYYLEDEETLKIWKKLLPENKIIPLGKKDNFWGP
                     AGETGPCGPCTEIYIDSGIDALCTNKDCKPGCDCDRFLEFWNLVFPQYNQQMDGQLLP
                     LKRRGVDTGMGLERITRIMQDKKSNYETDLFVPLIEKLEQIADEKYTEKTIKEFRIIA
                     DHSRAIVFLIGDGVYPDNEGRGYVLRRIIRRAYFQGTKIGLNQPFITSLLNPMIAIFD
                     GVYPEIVGRKEEIENIVIEEEKKFSGLLSSAKKIFDADTSKITDRVIPGKLLFKWYDT
                     YGIPRDLISELAEENHFSPDWQEFDHYLQKQQEQARKKSVFEQKKQVVFEINSLSETT
                     FTGYSNHEEHCNTVALYYLPDKKTWEIVLDKSPFYAEKGGQVGDKGIIRGNQWCFEVI
                     DSQIDSRGIIYHVGHFTVGTPQDVKEREKALASVSLKHRMDVSANHTATHLIHYCLRQ
                     ISNNQAKQAGSYVGPDRLRFDFLYPGQIDQEAIEKLELQVNEIIFENYPVKIEQMSYQ
                     QAVSNGAVALFTEKYGKTVRVVRTGDFHSELCGGIHVSRTSEIGIFRIISFSSIGENL
                     KRIEAVTRENAYQWFRKRWEILYQASNLLKTNPENLASSIIKLKKQVQELEEKIETIL
                     EKATTDIPSTLKKNSKNIVIKGYDATLMAGRVDGLTTDQLGKIADTVSSEANKLIILL
                     AGILDGRVLVICKVTPDISGMAPASLIVKHIAKMVDGNGGGKSEFAQGSGKDTRKIDE
                     IIKSAHDIVMNIVK"
     gene            complement(5505..5897)
                     /locus_tag="PLW07_00030"
     CDS             complement(5505..5897)
                     /locus_tag="PLW07_00030"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HOL48944.1"
                     /translation="MRIKEISEIKLRQNLEKKGYRDPELSETINYLKSRNFLNDYRFC
                     NLIIEKNIKKKRGLNYIRYLLTNDGIPETIISESLAKMYPESLEYTIARELVLSLKKP
                     FRKMLYILTSKGFSEQTIEKLKGEISEE"
     gene            complement(5932..7422)
                     /locus_tag="PLW07_00035"
     CDS             complement(5932..7422)
                     /locus_tag="PLW07_00035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HOL48945.1"
                     /translation="MEMKKFVGFGIIAGIIGGCLTFGAIYAVTKQGIQVGTNIKPVQV
                     VDTSGAYTLQNAFVQVAKMVKPAVVMITTEKTVTYRYWNPFGNDLFDQFFEDFGFSRP
                     RQQQPKTFKKKQEGLGSGFIVDEEGYILTNNHVIQGVDRILVKLTNDEKKYEAKIIGT
                     DSRTDLALIKINTGKKLPAATLGDSDTIEIGEWAIAIGNPMGLEETLTVGVVSAKGRN
                     VLGTSPYEDFIQTDAAINPGNSGGPLVNIKGEVIGINTAIIAPYVAQNIGFAIPINIA
                     KKVFRELKSSGKVTRGFLGVYLQPVTEELASSFGLKETKGALVSDVMPDTPAEKGGLK
                     PGDVILEFNGNSVTDTKDLQKKVADAKVGETATLTVWRDKKKINVQIKIAERPEEDTI
                     AKSESESTGKTWRGLKISDMTEEIRKQFNIDASLKGVIVINVEPGSPAEECEITAGTV
                     IRKINEWTVSNAKEFQTIISKIDKKSSVTLWVQQEGKNRFCVLKGE"
     gene            complement(7484..8662)
                     /locus_tag="PLW07_00040"
     CDS             complement(7484..8662)
                     /locus_tag="PLW07_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017434321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOL48946.1"
                     /translation="MGKKQINVGLIGYKFMGKAHSHAYRDISLFFDTSVVPVMKMICG
                     RHDVPLSIAQEKFGWQERTTDWKKITSSPEIDLVDITTPPDEHKDIAIEAAKEGKVVF
                     CEKPLANSLKEGYEMLEAVEKYKVKHAICFNYRKLPAVGLAKKLIEEGKLGKIYHVRA
                     VYLQDWILDPEFPLVWRLQKNIAGSGAHGDLNAHLIDMTRYLVGEFSEVVGVSETFIK
                     ERPLLIEKDDLNTGLVVTEKSDKKGKVDVDDATLFLARFENGAIGSFEATRFAPGRKN
                     GQKWEINGSKGSIVFNLERLNELEYYSVDDPACVQGFRTILATDASHPYAANWWPAGH
                     IIGYGDSFVNMVADVLNCVATNKMPSPNFADGVKCQEVLEAVEESIMQGKWVKIKSKV
                     "
     gene            complement(8677..9705)
                     /gene="recA"
                     /locus_tag="PLW07_00045"
     CDS             complement(8677..9705)
                     /gene="recA"
                     /locus_tag="PLW07_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOL48947.1"
                     /translation="MDKEKALSMAIEQIEKNFGKGSIMKLGEKTRLEVDAIPTGAISL
                     DIALGVGGIPRGRITEIFGQESSGKTTLALQVIAEAQKNGGQAAFIDAEHALNPNYAQ
                     KLGVNLDELLISQPDTGEQALEIAESLIRSNAIDVVVIDSVAALVPRAELEGEMGDSV
                     MGLQARLMSQALRKLTGYISKSQTAAIFINQVREKIGVFFGNPETTPGGRALKFYSSV
                     RLEVRRIENLKTSQGEMYGIRIKTKIVKNKVAPPFKEAEIDILFDSGVSKEGSTIDAG
                     LSLNVIEKKGNHFYFGQKALGHGRESTIKFLKDNPDVYQQLIKKLRQKAGLEKEVEQT
                     GQEKGKTK"
     gene            complement(9707..10264)
                     /gene="thpR"
                     /locus_tag="PLW07_00050"
     CDS             complement(9707..10264)
                     /gene="thpR"
                     /locus_tag="PLW07_00050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOL48948.1"
                     /translation="MRVFSGIKLTDILTDELEPITVMLAKQKNAIKIVPAKNLHITLR
                     FLGNIHEEKYQDFLHNIQNAFTSLIPFSIDVQGIGFFPDRNHIRVIWVGVEQHPAILL
                     IHDILERAATKIGLPPENKFHAHITIGRTRSAIDSNFIHDLETKFQHEHWGRMEINHI
                     TIFESILHQTGPEYKELVNIPIGGN"
     gene            complement(10261..10923)
                     /locus_tag="PLW07_00055"
     CDS             complement(10261..10923)
                     /locus_tag="PLW07_00055"
                     /inference="COORDINATES: protein motif:HMM:NF017373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL48949.1"
                     /translation="MNEFLLAPTKELISRLGVAISKLLFGLLVFIVGWIVAKVFKILA
                     TKFLKMLKIDKLVEDSGFREILLRGQITKEPSELMASVIYWLLLIVVVFLSINVAGVP
                     IPPEVIENLLSFIPKIIIGLILFIASLLVGNIVEGVVNTAAGNAGIQKSYILGKTTKV
                     VIIIFGVVLALDEMGIGGNFVTSVFNIILASGALAFALAFGLGAKDVVKNWLEEFLTK
                     KQ"
     gene            complement(10977..11975)
                     /gene="ilvC"
                     /locus_tag="PLW07_00060"
     CDS             complement(10977..11975)
                     /gene="ilvC"
                     /locus_tag="PLW07_00060"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOL48950.1"
                     /translation="MAKIYYDKDADLSVLKNKTVGIIGYGSQGRAHALNLRDSGVNVM
                     VSELKGTNNWILAEKDNFTPASTAELVKKADIMMMLTQDNLQPVVYENEIKTNIRNGQ
                     ALGFAHGFSIVYSQIKVPSSIDIFMVAPKGPGDLVRTQFTEGKGVPCLVAVEQNPTGN
                     ALKIALAYAKALGGTRCGVVETTFQEETETDLFGEQVVLCGGVTELIKASFETLVEAG
                     YQPEIAYYETCHELKLIVDLIIERGIQGMRKVISDTAEYGDMTRGPRIIDESVREEMK
                     KILKEVQDGVFAREWILENRVGRPVFNALAKKAEKHLIEEVGAKMRSMMPWLKKKQ"
     gene            complement(12009..12512)
                     /gene="ilvN"
                     /locus_tag="PLW07_00065"
     CDS             complement(12009..12512)
                     /gene="ilvN"
                     /locus_tag="PLW07_00065"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011498832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOL48951.1"
                     /translation="MIEKNQHIISVTVENRSGVLARIAGLFSARGYNIDSLAVAETED
                     PTVSRMSIAVSGDEKILEQIYKQLNKLVDVIKVQDLTSEEHIERELVLLKVNTANSNV
                     RNEIVQLVNIFRAKIVDVGRKSLTIELVGSKPKVDAMIDLMKGFGVKEITRTGRIAIC
                     REAGKEK"
     gene            complement(12513..14183)
                     /gene="ilvB"
                     /locus_tag="PLW07_00070"
     CDS             complement(12513..14183)
                     /gene="ilvB"
                     /locus_tag="PLW07_00070"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOL48952.1"
                     /translation="MKGAQIIVECLKKEKVEVIFGYPGGVVLPLCDALYDSGIRFILT
                     RHEQGAAHAADGYARASGKVGVCLATSGPGATNLVTGIATAYMDSIPLVAITGQVATQ
                     LIGNDAFQEVDTTGISRPITKHNYLVKNTEHLTRVFKEAFFIAKTGKPGPVLIDIPVD
                     VQRGEVDFDYPETIEIRSYKPVYNGHPVQIERALSLIETSRQPLILAGGGVISANASE
                     QLIKFVEKTNIPVALTLMGLGSFPTEHPLHLGMVGMHGTRYGNNAIMETDLLIAVGCR
                     FDDRVTGKISEFAPHAKIIHIDIDPASIGKNVLVEIPIVGDVKNVLSQMLKNCPKTKI
                     EEWLAKIQQWKEKYPLTYKDTDGLKPQFVVETISELTQGEAIIATEVGQNQMWAAQYY
                     RFKHPRTFISSGGLGTMGFGFPAAIGAQVACRDKIVVDIAGDGSIQMNIQELSTAVSE
                     KIPVKVVILNNCHLGMVRQWQELFYNKKYAFTCLKGKQPDFVKIAEAYGATGYRVTKK
                     AELRKILTKAFSHNDGPVFVDCHIEPEENVFPMVPAGASLKEMIGGLA"
     gene            complement(14338..15609)
                     /locus_tag="PLW07_00075"
     CDS             complement(14338..15609)
                     /locus_tag="PLW07_00075"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014829494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOL48953.1"
                     /translation="MVTVVVGTQWGDEGKGKIIDTLASQHDIVARYQGGANAGHTVVC
                     NNQKFVFHLIPSGLLHKNVVGVLGNGVVIDPSSFLQEMTDLENHGIDFTNRLFIAENA
                     HLTLPYHKILDQIEDNKRGKDKLGTTGRGIGTTYTDKYGRMGVRVVDFLNEEVFVKKL
                     NIALELKNYLFQQYYKESAVRVEQLVEEYSKYREKLKNLTIDASAYLNDALENGKTVL
                     AEGAQGTFLDIDFGTYPFVTASNPVSGGACTGLGISPKKITKIIGIAKAYTTRVGMGP
                     FPTEIKDELNEKLRTAGNEFGATTGRPRRCGWFDAVIVKYAAMLNGLDELVITKLDVL
                     SGLKTIKIGIKYYHNGNPIDKYPLDTNVFENIEVEYEEFQGWDENINDVPDFHHLPTN
                     AKKYIERIEDLVNTRITAISVGSSREQIISR"
     gene            complement(15610..16689)
                     /locus_tag="PLW07_00080"
     CDS             complement(15610..16689)
                     /locus_tag="PLW07_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HOL48954.1"
                     /translation="MKKHTVTLIPGDGIGPELTEVTKECIKATGVSIDWEECEAGIPA
                     IEKFGDPLPENTIQSIKKNKVCLKGPITTPVGSGYRSINVALRQIFNLYACVRPFKVY
                     RGARTLYENIDLVVVRENMEDLYAGIEFEMGKSETLSVIKEIENLIGKKISNDSGISI
                     KPISITGTEKIVKFAFEYARKNKRKKVTAVHKANIMKFTDGLFLDVARKVSLNYPDIE
                     FEDRIVDNMAMQLVQKPQKYDVLACPNLYGDILSDLCAGLVGGLGLSPGANYGDEVAI
                     FEPTHGSAPKYAGKNVVNPSAQILAAVLMLQYIGEVESAEKLENALSEVIEEGKHTTY
                     DFRPEGTAGVSTDEMGKAIIKKLGA"
     gene            complement(16686..17183)
                     /locus_tag="PLW07_00085"
     CDS             complement(16686..17183)
                     /locus_tag="PLW07_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOL48955.1"
                     /translation="MILTGKVHKFGDDINTDYIISGRYKFKTLDMTELASHIFEDIDP
                     DFSKKINPGDFIVAGNNFGCGSSREQAVLALKYAKIGAIIAKSFARIFFRNGINVGLV
                     LIEANTDPISQHDILQIDLLKGHIFNESQNIHVPVKPLPEFLLKIINEGGIINYLKKY
                     KDFQI"
     gene            complement(17251..17898)
                     /locus_tag="PLW07_00090"
     CDS             complement(17251..17898)
                     /locus_tag="PLW07_00090"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOL48956.1"
                     /translation="MVLQKLKFLLFHHIIDPIFPEKCCGCGREVAIKKYCFCIDCLNH
                     ITIAPSLHERIFSLALYEGPVMNAIHTYKYKKRKWIAFSFATWMNDFITQHSEIKFDI
                     IIPVPLHPVKEFKRSFNQSWLIAFHLGKMQNKPAVYHALVKTKNNRSQTNLDINERKE
                     NVANVYKVRNTSIIKDKKILVIDDVYTTGATIEEVYKTLKKNGAREVKVLTVAKA"
     gene            17897..18970
                     /locus_tag="PLW07_00095"
     CDS             17897..18970
                     /locus_tag="PLW07_00095"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOL48957.1"
                     /translation="MRLQKKYIYTEFFNFYVIAFFIFAFIFMMRALLLLSDLIINHFF
                     TPVTVVRFFLFSFFEICPDIVSLSFLTAVTGTVARMNMEREYWVFLTSGVSANQIVRY
                     FLLLGVIFIFLEIWLCLFLSPNSTYGKKLLLNQAHLDEPMKLFRNQTLVKDFPGLTIY
                     VERVMNRNFYNVVICYREGSDSVCIIKAEKAHLRIDRDGFLCLFLENGFLETHSIKKA
                     TLLVKVDFDVYTFSLPYKRIVDAFPQRKIKEMNFFMLLNSIDVRTNRNEILVMLFKKI
                     FFATLPLFYVFMGFYTGIGIRATGYLQILGIGLFIGLGSYFMILVGEGIAFKTNSSFM
                     FFVTPLIFIFAIIAVKRKFSHVA"
     gene            18960..19979
                     /locus_tag="PLW07_00100"
     CDS             18960..19979
                     /locus_tag="PLW07_00100"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOL48958.1"
                     /translation="MLLREKHILLDTFKFFLLLLTAFAILLFILQIFWTIADARHTRV
                     FLWKDCVVNFYDSFLVVIPFITILSAFLVFSEMEKFKQISIFELKGISELQIFKIFVI
                     FGFLCSLFSFIAGLLTPYQNEIKNNSRKTDSLSILTESVCFWATDYKQQDIVENLIIS
                     ENRSNVTYYAKKAKFFEKSIILYDGKIVYGDRTTRHFQQFTLETDFCPRALLLYAVGF
                     PERWTFFKLHGLLKQVASVGIKSKSDWIFLYSKISYPMLNIFIILLLFPFFHYRRSMA
                     KTRIFSVAFTGTLMVYSLYSFGLSLGRAEIIPWQVAPWVSHLILTIFFILYLSAVRKK
                     MYNFI"
     gene            20014..22110
                     /gene="glyS"
                     /locus_tag="PLW07_00105"
     CDS             20014..22110
                     /gene="glyS"
                     /locus_tag="PLW07_00105"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HOL48959.1"
                     /translation="MKTEHLLIEIGCEDLPSWSGQHFISRFKPLFLGELNNYKLTADD
                     VMFFFTPRRFVIFIKGLSSKIPPERREITGPRYEAAFDKDGQATIAAAGFAKAHGVSV
                     SSLKVKNVNGKKVVYVTKTIPGVATKDIIPDILVRTLRKIDIPKGMRWNESQEVFYRP
                     VRWMLAIFGNQNLDVNFGGIKSSRYTYGHRILRPNRIRVQNWEDYFDKIQKSFVLLGE
                     DLRQQFILNLISTNLKENENFEESMGKDLVNLIEYPRVIRCNFPSIDYNLPEEVLRVL
                     IAKAKGIPIFCGEQLKKEYFVISDGNDNENVRVNYENLLKTRILDAQFFYNSDISVPF
                     DEFGKKLDKIVFHQKWGSVAKKVERLKGLAFEFSEIMGLDSEQKKTLIRSAEICKNDL
                     ASEMVREFPDLHGTMGAIYAKLSGESEDVCRTIAEYMLPASPGDKLPQGICGILLGII
                     DRLDYVVAFISAGVDVSGSEDPYGLKRIAGGLFPLIYVSGFEYDYRQIVRKILEYYDI
                     PAEEKNSISSKIDEFLIQRYESHLESEGFPRGLRRSVLTIDGMNFLKIRKKLEALKNF
                     IKNDIDAEQILIPVTRIANILRQATERKIQIPEFHQDLIEESGERILAEVYNTYAEKS
                     EGLLAQMDYDNFLKILSGLKEPVDNFFDSVLVMCPQEVLRNNRLALLKKFNDVFLKFA
                     DFSYIREEDIKNAQKY"
     gene            22094..23332
                     /gene="glyA"
                     /locus_tag="PLW07_00110"
     CDS             22094..23332
                     /gene="glyA"
                     /locus_tag="PLW07_00110"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOL48960.1"
                     /translation="MLKNTDSDIAMLIKREIQREKETINLIASENYVSKAVLMAVGSI
                     LTNKYAEGTPWKRYYDGCENVDEIESIAIARLKKLFNAEHANVQPHAGSQANMAVYFA
                     ALKPGDVILGMDLAAGGHLTHGTKVNFSGMIFKSFYYGVDRESERLNYDEILSIAKKV
                     RPKLIMCGASAYPRIIDFEKFSDIARNVGAYLCADIAHIAGIIAAKLHPSPVECSEFV
                     TGSTHKTLRGPRGGFILCKKGFAQEIDKAVFPGIQGGPLMHVIAGKAVAFKEAMDKKF
                     YFYQKQLLENCSLMAKGLIEKKYRLVTGGTDNHLILIDLRNKMITGEQAQKTLGMVGI
                     TVNRNAIPYDPLPPNIASGIRVGTAAITTRGMKKGDIEKILDFIDEALYYRDDCNKLA
                     MIRKQVKKFASQFPIYPEQR"
     gene            23329..23799
                     /locus_tag="PLW07_00115"
     CDS             23329..23799
                     /locus_tag="PLW07_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009076873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HOL48961.1"
                     /translation="MKNKPRPGWDEYFMKIAHLVSERSTCLRRKVGAVLVKEKRILAT
                     GYNGAPSGVTHCEKAGCLREELGIPSGERHELCRGLHAEQNAIIQAAYHGVSIKGSVL
                     YITCHPCSVCAKMIINAGIKEVVIFEGYPDKLAKIMLEEASIVIRYIGKDVSKH"
     gene            23814..24062
                     /locus_tag="PLW07_00120"
     CDS             23814..24062
                     /locus_tag="PLW07_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL48962.1"
                     /translation="MSKNILLFGKQDCGLCQGWKKKLENFNIPFTYLDTENPDNLTEF
                     TLMGLTKIPALVIGENKFEGIRPSDLSIDEIKKMMENE"
     gene            24055..25671
                     /locus_tag="PLW07_00125"
     CDS             24055..25671
                     /locus_tag="PLW07_00125"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOL48963.1"
                     /translation="MNNNDTMVRNLPPIILSVLHQRGIKTRKQIQEFLDPSVSSLSDP
                     FSISDMEKGCKILEQAIYSKKNIFLYADGDVDGIAGAAMFVNLFRKMKIPFDIKLTHR
                     LEDYEIEPDFINRIRNLGYELLITIDTGTTSNELIEYCEQLGFPLIIIDHHKGEMLKN
                     LKHVVIINPSLGREDSDFDVLTASGLSFKVIQALKSFSPFFPDEIFASSIELATIGTL
                     SDYGFLTGENRVIVKIGLNGLENTSIPGIETFREFFYVPGKNSDVESITHYLNPKLNT
                     PGRFGRPELTLKVLTANKEEEIQNVFEEIEFFEKEKQKILRRFSCSIEKLKNPECPLF
                     LLFENIPASFSGTFAARTSEKFQLPALVAIKNGDFLQGSARSYDRADLYELFNNFKNY
                     FISFGGHKNAVGFKMKIDFLNELKHAWKQLRLCSISSKQSVKPLEADFEALTIPVFEN
                     IQQLKPFGPGNPPVLFISNPVECLKITGCKGKKTIAWVKQGNKMFEVHFSKGFKMPGG
                     PISITYVPHLRKSEGLYIPWLDVKNYSTTF"
     gene            complement(25653..25850)
                     /gene="rpmB"
                     /locus_tag="PLW07_00130"
     CDS             complement(25653..25850)
                     /gene="rpmB"
                     /locus_tag="PLW07_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOL48964.1"
                     /translation="MKCEICGKGQKTGNNVSHSNVKTRRTWKPNVQKLLVEYQGKTRR
                     LAICTQCLKSGKVKRPQKVVE"
     gene            complement(25863..27635)
                     /locus_tag="PLW07_00135"
     CDS             complement(25863..27635)
                     /locus_tag="PLW07_00135"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOL48965.1"
                     /translation="MSKKKKEQENISQMLKKDEETIGFRTLVTIVSILFFGIAPLLNF
                     KPAYDYTIIKDILGALSVAIFAAALFLRKETISIHFTGFLAGTLFFAFILMGCFYAPV
                     KYGAAQALENYLLYYLLFIMGMLWKWEKKDAFIWASAMVIASITAFVQYPKTHYPIST
                     FGNPNFFAGHLLMPVFLTLYFFKKKLNTIAAIYFIITLAALILTKSRASLFALATACS
                     FTSFFLLRNHKIPWLKYIGFVIIVFGTAIMWHKIQTQFVQDIRYYIWKGTWNLIKAKI
                     VTGWGTGNFIFYYPYFRFREYFLRPQATPITNHPHCQYLEIWSENGIIGLVFFFIIIV
                     VAIYNGLKTKDEESNNTFLFASSGIIAVLVDNILSTNLTNTSTAMYFWFLLGLCFSLK
                     KTTTIQISGFLKKTTAAIVMIAMITLAGWKTFYRLVPEVYLKKAIAAREANNFITAIE
                     NYKKVCKINPYNAVALYKMAYAYGQIGDIKNAEEVYLKINNNIFPHFAKTDANLGTLY
                     MQTQNFKRAKVYYEMALGFNPYDVDVLLGLASIKIFQEKDIKGAKEYLEKVMSINPKH
                     EYVQYLLKSIPELKNIVTNETKNR"
     gene            complement(27650..28942)
                     /locus_tag="PLW07_00140"
     CDS             complement(27650..28942)
                     /locus_tag="PLW07_00140"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HOL48966.1"
                     /translation="MDKKILIFLDFIIILISMLLGYAIRFSWHIFPDKGIAPLKPYIH
                     ISIFAALIWIVMLNVNRIYKEHKFINLCFELSKIIQSSFYSAIIISALTFFYRGFYYS
                     RIAIILGILISFFILSLVHLIINVTLAKEDFTFYLVGDNRDMTPIVKRLKLHGIPGNR
                     IIFQHVEQLQFSVQNSDRPDNIRVIICLEDFQKIQQIARICSNSGIQYYVYPRTSRVF
                     LSGGRVEEIYGIPVITTDILPLEIWHNKVIKRFFDILFSMAFLTISMPFLFLIATIIK
                     FSSDGPVFFIQERTGYRNKKFKIFKFRTMVKGAENILPYTLEKDSRITKIGTWLRKSN
                     IDELPQLFNVLKSDMSIVGPRPLSTEDRLFFSIPGFYERMKILPGITGWAQIHGLKGG
                     QVEPEERFRYDLYYAENWSIWLDFAIIIFTGFLFFRDK"
     gene            complement(28935..30035)
                     /locus_tag="PLW07_00145"
     CDS             complement(28935..30035)
                     /locus_tag="PLW07_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HOL48967.1"
                     /translation="MKIAILGIRGIPARYGGFETCAEKIAEYLGKKGHKIFVPCRKYL
                     YPDKPLPGDNIQVCFPFCIRGKITETFSHTFFSMLKTIIWSPDVILLFNAANSPLAIF
                     GKIYGKKFVINVDGFEWKRQKWGFCGKTYFKFASCFSTFIADSIVADSHKIAEYYDSR
                     FHKKSVYIPYGADIFYSKSPEIIKSMGLQPKTYFFNGSRLEPENNQELMIKEFQLANI
                     NNVLAISGDIRKKNSYVKNILNIASQQIKFTGPVYEKKAYLELQANSLAYIHGNEVGG
                     TNPALLSAMGCGALILALDVPFNREVLGDCGLYFTKKSGSLANLIKQMVESPEKFSSL
                     GDKARERVKQFYRWDDVIQKYEQLLISVENNG"
     gene            complement(30044..31162)
                     /locus_tag="PLW07_00150"
     CDS             complement(30044..31162)
                     /locus_tag="PLW07_00150"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOL48968.1"
                     /translation="MIKIGIEAHAIGMKQGGNERYIEGLLRGLSKLNPPDLQFIVFLN
                     HMAEIPSFLKGHPSFSFEKVSTSSIKRLMLDLPSKIKRTNIDVLHTQYHIPPFVQIPS
                     VITLHDVSYLIHPEFFPFFERIKMKTLMPSSIRKAKKIITVSQFSKREILKKYGDIDE
                     KVCITYNGVSEEFKPVNEEKIQQVLTKHKIFRPYIISVSNLQPRKNLQGLISAYSLIL
                     KKKEDFPCCLVITGKKLWLYDEIFSAGEPTIKEKILFTDYVENDDLIALYSGAEMLVY
                     PSFYEGFGLPVLEAMACGCPVIASASSSLPEIVEEAGILVDPYNSQEISSAILTLHQN
                     GNLQKELKEKGLKQAKKFSWMECAKKTVEVYRNLVSLL"
BASE COUNT         8850 a         6009 c         5751 g        10630 t
ORIGIN      
        1 aaaaaatata atgtggcagt aattggagca ggtcttgttg gaaaaaaaat ggttgagacc
       61 ttaatcgaaa gaaatttccc ttttgctgat ttgaaagttc ttgcgacaag agaaagagaa
      121 gaaattattg ctggaagacg ttttcaggtt gagaaaacgt ctgaagattc ctttactggg
      181 atagattttg cattctttgc cggtactgaa ggagaaaagg gagcaagcat tgagttcgga
      241 tggaaagcag ttgaaaaagg agcaattgta attgataatg gaggagattt tagaatgcat
      301 ccagatgtgc cgctggtgat tcctgaggtg aatgctgaac atttgaaaaa acataagggt
      361 tttatagcaa atccaaattg ttctactatc attatgttaa tggctgttgc gcctttaaat
      421 gctcgttaca gggtgaaaag aattgttgtt tcaacatacc aggcagtgtc tggtacaggt
      481 agcaaggcat tggaggaact tgaaaatcaa tcgcatcagt atgaaaaaaa tcaggaaatg
      541 actgttcggg tttatcccca caggatattg tttaacgtga ttccgcatat cgggaacctt
      601 tcagaacaat ttgcgggata ttactcagaa gaagtaaaaa tgctacttga aacaagaaaa
      661 attcttgatc gacctgatat gctggtttct gcaacgtgtg tcagggttcc tgttttcaat
      721 ggacattcag aagcgattac gattcagttt gaaactgatc ctgacgttga tgaatgtcgt
      781 gaaattctat catccgcaaa aggagttaaa ttattggatg accccggaaa ctcacaatat
      841 ccgataccca tagaagtatc tggtaaggac gatgtttatg ttggcaggat tagaaaaaac
      901 actgcacttg atcatgctat tgatatgtgg gttgttggag ataacattaa aaaaggcgcc
      961 gcacaaaatg ctgtccagat tgcggaagca atgattgaaa tgaatcttat ttaacactat
     1021 ttcattgtcg gagaacttca tattgatttt caataagtgt ttggattact gcatagtttt
     1081 ttcttattga tgttttatac caaaatacct gttgccaatt ttttgaatgc gaaacggcga
     1141 aggtttgttt ttcttgaacc caagttttct gataacatcc gggattcctt caagtgctga
     1201 gaaagttcca cgaggagcca cagcggttcc tgtgtgtttt tccattgttt ttatgtcgag
     1261 aacaagccaa tttttattta aaaatacaac ctgaagatct tgatttactg ttccatatcc
     1321 cggattggtg ttcacgaata ctgtttcttc tttgatatct ggaaaccaga ttactgttgt
     1381 ttgaaattgt tctgatgtcg ctccgcagaa accctgatgg ctcagttgct ctgtttttgc
     1441 tacaagaatc gtgacatcag aaattttttc ttcaactttt ggccatagtt caaaagggat
     1501 tcttatgtct gtacctgata tgagacaatt aacgaagaaa tatagagaaa gcaggaataa
     1561 acggaaaaaa gtgattttca taaagtaagc atgctgtaaa tatcataccc tttaattttt
     1621 tctctaccat taagatcttt cagttctatc aaaaaaagaa tcttttcgac ttttgctcct
     1681 gcacgttcta tcaaggagca tgcggctccg gcagtacctc ctgtggcaag cacatcgtca
     1741 attaaaacaa ctgtttcatt cttttcgaac gcatctgctg gaatttctat tgtgtcctca
     1801 ccatactcaa gcgcatacgt atgttttatt gttttgcatg gaagtttacc taattttctt
     1861 ataggaacaa atcctttgtt caatttacag gcaagggcgc caccaaagat aaaaccacgg
     1921 gattctattg caacaatttt gtttattcca tcaggcacct gttttgcaaa caattcaatg
     1981 cttctggcga acaggtcttc attttttatt aaaggcgtaa tatctctgaa caaaactccc
     2041 ctctttggaa agtctggtat tgttcttata gcatcatata attctttcat ttttcatctc
     2101 ctttatttcg ttatgcaaat gtttcaggca tgttttaata atttggtgta ctctctgtct
     2161 ggaaaaaccc atctgttctg cgatttcttt ataggacctt ggaataccat catcaattcc
     2221 ataatgcaat ttcagagcct ttagccatat atcaattgaa ccacttttta tctttttttg
     2281 caaccttcta aacaatttgt tcagtatatc tttatctacc agtatgctgt cccattgctt
     2341 cttgcttgat gtaaaatttg taatatcaaa taggtcatag tcttcatttt ctgaatcctg
     2401 aaaaaaagga gaaatttttg ttgattcaaa ataaggcttg tatttcaaaa aggttgggac
     2461 atccagtttt aatagtgatg ccatttcagt atttgtggga catctatttt cttcaagttt
     2521 gactttttca atgacgtttt tgtattttgt tttgagtttc ttgatttttt ccggaacctt
     2581 aaaggaactg gttgacattg ctcgcataat gtaacgtctg acccagaaat acgcatatgt
     2641 cccaaatctt gcgtgttttt tggggtcaaa cttttttatg gcctcaatca atccgatatt
     2701 accttcagaa atcaagtctt caagaatgtc atttgaaaac gcatatcttc tggcaattga
     2761 gacaacaagc ccctgattca ttttcatgaa tttttcctgc gcgtctctgt caccctgttt
     2821 tatttttttc agtaattcca gttcttctat cctgctcggt tttttcaaaa tttttcctgt
     2881 tgaaatttta tttttcattg gattatttta cgatattcat aactatgtca tgggcacttt
     2941 ttataatctc atcgattttt ctcgtgtctt ttcccgaacc ctgagcgaat tctgattttc
     3001 caccaccatt accatccacc attttcgcga tatgcttgac aattaaagat gcgggtgcca
     3061 tgcctgaaat atcaggcgtg accttacata taacaagaac ccttccgtca agaataccgg
     3121 cgagaagaat gattaattta tttgcttcag aggaaacagt atcagcaatt ttaccaagct
     3181 ggtcagtcgt taatccatcc actcgacctg ccatgagcgt tgcatcatat ccctttataa
     3241 caatattctt gctgtttttc ttcagcgtgg acggaatatc ggttgttgct ttttcaagaa
     3301 tggtttcaat tttttcctca agttcctgaa cctgtttttt taatttaata attgaagatg
     3361 ccaggttttc aggattggtt ttaagcaggt tacttgcctg gtacagaatt tcccatcgtt
     3421 ttctgaacca ttgataggcg ttttctcttg taacggcttc tatccttttg agattttcac
     3481 caatagaaga aaacgagata attctgaata taccgatttc agatgttctg gaaacatgaa
     3541 ttccaccgca caattctgaa tgaaaatcac ctgtcctgac caccctcact gtttttccat
     3601 atttttcagt aaataatgca actgcaccat tacttactgc ctgttgataa ctcatctgtt
     3661 caatttttac agggtaattt tcgaaaataa tttcattgac ctgtaattcc aatttttcaa
     3721 ttgcttcctg gtcaatttga cccgggtaaa gaaaatcaaa tcttaatcta tccggtccta
     3781 cgtatgatcc tgcctgtttg gcttgattgt ttgaaatttg tcttaaacaa taatgaataa
     3841 gatgagttgc agtatgattt gctgaaacat ccattctgtg tttcagtgaa accgaagcca
     3901 atgctttttc tcgttctttt acatcctgtg gggttccaac agtgaagtga ccaacatgat
     3961 aaataattcc tcttgagtcg atctgggaat caataacctc aaaacaccat tgatttcctc
     4021 tgataatccc tttatcacct acctgccctc ctttttcagc ataaaatggt gatttatcca
     4081 gcacgatttc ccaggttttc ttatcgggta gataatacag ggcgacagta ttacaatgtt
     4141 cttcgtgatt actgtatcct gtaaaagtgg tttcgcttaa tgaattaatt tcaaacacaa
     4201 cctgtttttt ttgttcaaaa acagattttt tccttgcctg ttcctgttgt ttctgcaaat
     4261 aatgatcaaa ttcctgccaa tctggtgaaa aatggttttc ttctgcaagt tcagaaataa
     4321 ggtcccgtgg gattccatat gtatcatacc acttaaaaag aagttttcct ggaatcaccc
     4381 tatctgttat ttttgatgtg tccgcatcaa aaattttttt tgcagaggaa agcaatccag
     4441 agaatttctt ttcttcttca ataacaatat tctctatttc ttcctttctc ccaacaattt
     4501 caggatatac gccatcaaaa attgcaatca ttggattcag cagggatgta ataaaaggtt
     4561 ggtttaaacc aatttttgtc ccctgaaaat acgctcttct tatgattctc ctcagcacat
     4621 atcctctacc ctcattgtca gggtaaacac cgtcgccaat gagaaataca attgcccggc
     4681 tatggtcagc aatgattctg aattctttga ttgttttttc tgtatacttt tcatctgcga
     4741 tctgttcaag tttttctatt aatggtacga aaagatctgt ttcataattt gatttcttat
     4801 cctgcataat tctggtgatt ctttcaagcc ccatgcctgt atcaactcct cttctcttta
     4861 gtggtagcaa ctgtccatcc atctgctgat tatattgcgg gaaaacgaga ttccagaatt
     4921 caaggaaacg gtcacaatca caacctggtt tacaatcttt atttgtgcaa agggcatcaa
     4981 ttcctgagtc aatatatatt tcagtacagg gtccgcaggg tcctgtttct cccgcaggtc
     5041 cccagaaatt atccttcttt cccagtggta taattttatt ttctggcagt agttttttcc
     5101 agattttcag ggtttcttcg tcttccaggt aatacgaagc ccagagtttt tctttactga
     5161 ttttcagtat cagggtaaca aattcccatg cccaatgaat ggcttcttct ttaaagtaat
     5221 caccgaaaga gaaattgcca agcatttcaa aaaaagtgtg gtgtcttcca cttaatccaa
     5281 ttttttcaag atcactgtct tttcctcctg ctcgtaggca tttctgacag gacgttgctc
     5341 gcgagtaagg cagtggcact tctgttgccc agaatttttt aaactgtacc atgccagcgc
     5401 tcgtaaacaa tagggttgga tcttcaaatg gtattagact gctactcggg acaactgtat
     5461 ggcttttttt ttcaaaaaac tccagaaatt tcttgcgtat ttcattactc ttcactgatt
     5521 tcccctttca atttttctat ggtttgttca ctgaaacctt ttgaagtcaa aatatacagc
     5581 atctttcgaa acggtttttt gagtgacaat actaattccc tcgctattgt atattctaaa
     5641 ctctctggat acatttttgc aagtgattca gatatgatcg tttcagggat gccatcattg
     5701 gtgaggagat atcggatata attcaaacct ctcttttttt taatgttctt ttctataatc
     5761 aggttacaga acctgtaatc gttcagaaaa tttcttgatt tcaggtagtt tattgtttct
     5821 gatagttctg gatccctgta tccttttttc tcaaggttct gcctgagttt gatttcagaa
     5881 atttctttaa tcctcaatat tttcagggca tcccgcagga tattattatt tttattcacc
     5941 tttgagaacg caaaatctat ttttcccttc ctgttgcacc cacagcgtga cagaagattt
     6001 tttgtcaatc ttactgatta ttgtttgaaa ctcttttgca tttgagacgg tccattcatt
     6061 aatttttctt atcactgttc ctgccgtaat ttcacattct tctgcagggg aacctggttc
     6121 tacattgata actattactc cttttaatga agcatctata ttaaattgtt ttcttatttc
     6181 ttctgtcata tcgctgattt tcagacctct ccatgttttt cccgtggact cactttcgga
     6241 ttttgcgatc gtatcttctt cgggtctttc agctattttt atctgaacgt taattttttt
     6301 cttatctcgc catacggtca atgtcgccgt ttctcccact tttgcatcag caaccttttt
     6361 ttgtaaatct tttgtgtctg tgacagaatt tccattaaat tcaaggatga catcgcctgg
     6421 tttcaaaccc cctttttcag caggtgtgtc aggcattaca tctgatacca gggctccttt
     6481 tgtttctttc aaaccaaaag aagaggccag ttcttcggta actggttgaa gatatactcc
     6541 caggaatccc cgggtaactt ttcctgacga tttcaattct ctgaaaactt tttttgcaat
     6601 gttgattggt attgcaaagc caatattctg ggcaacatag ggtgcgataa tggctgtatt
     6661 tattcctatt acctcgccct ttatatttac aaggggtcct cctgaatttc caggattaat
     6721 agccgcatcg gtttgtatga aatcctcata gggacttgtt ccaagcacgt ttctcccctt
     6781 ggcacttact actccaactg ttaatgtttc ttcaagtccc atcgggtttc ctatggcaat
     6841 agcccattcg ccaatttcga ttgtatctga atcacccagt gtggctgcag gaagtttctt
     6901 tccggtattg attttgataa gggcaaggtc tgtcctggaa tctgtaccaa taatttttgc
     6961 ttcgtacttt ttttcatcat tcgtcagttt cacaagaatt ctgtccactc cctgtataac
     7021 gtgattattt gtaaggatat aaccctcctc atctacaata aatcctgagc caagtccttc
     7081 ctgttttttc ttgaatgttt ttggttgctg ttgtcgcggt cgcgaaaatc caaaatcttc
     7141 aaaaaattgg tcaaaaagat catttccaaa aggattccag tacctgtatg taacggtttt
     7201 ttctgtcgtg atcatcacca ctgctggttt gaccattttt gcaacctgta caaatgcatt
     7261 ctggagggta tatgcacccg aggtatcaac cacctgaacc ggttttatat tggttcctac
     7321 ctgtatacct tgttttgtaa ccgcatagat cgccccaaat gttaaacaac ctccaataat
     7381 tcctgctatg ataccaaacc cgacaaattt tttcatttcc atgattacct ccttgatttt
     7441 tttgtttgct caaattttta gatgcaagaa atcaaaagga ggtttagact tttgatttaa
     7501 tttttaccca tttcccctgc atgatggact cttccactgc ttccagtact tcctgacact
     7561 ttactccatc tgcgaagttt ggagatggca ttttgtttgt tgcgacacaa ttcaatacat
     7621 ctgccaccat gttcacaaac gaatctccgt aacctataat atgtcctgct ggccaccagt
     7681 ttgcagcata aggatgggat gcgtctgtag caagaatggt gcgaaatccc tgaacgcacg
     7741 cagggtcgtc aactgagtaa tattcaagtt cattcagccg ttccagatta aaaacgatgc
     7801 tgcctttgct tccattgatt tcccatttct gaccattttt ccttccagga gcgaaccttg
     7861 tggcttcaaa agaacctatc gcaccatttt caaatcttgc aagaaaaagt gtggcatcat
     7921 caacatcaac tttgcctttt ttgtcagatt tttctgttac aaccagacct gtgttcaaat
     7981 catctttttc tattaaaagg ggtctctctt ttatgaatgt ttcgcttacc ccaactacct
     8041 cgctgaattc tccaacaagg tatctggtca tatcaataag atgtgcattc aaatctccgt
     8101 gtgctcctga accagcgata tttttttgca atcgccacac aagcggaaat tcagggtcaa
     8161 gtatccagtc ctgaagatac acagcacgta catggtaaat ttttccaagt ttgccttctt
     8221 caataagttt ttttgccagt cccactgcgg gcagttttct ataattgaaa cagattgcgt
     8281 gtttgacctt gtacttttca actgcctcaa gcatttcata tccctctttt aaggaatttg
     8341 caagtggttt ttcacagaat acgacttttc cctcctttgc ggcttcaatt gcaatatctt
     8401 tatgttcatc aggaggcgtg gtgatatcca ccaggtcaat ttctggactg ctcgtaattt
     8461 ttttccagtc ggttgttctt tcctgccatc cgaacttttc ttgcgcgatt gataatggga
     8521 cgtcatgtct tccgcatatc attttcatta ccgggacaac agacgtatca aaaaataaag
     8581 atatatccct gtatgcatgt gagtgggctt ttcccataaa cttataacca atcaaaccta
     8641 cattgatttg ttttttcccc attttcccct cctatgttat tttgtttttc ctttttcctg
     8701 acctgtttgt tctacctctt tttccaggcc agccttttgt cttagttttt ttatcagttg
     8761 ttgatatacg tcgggattgt ctttcaaaaa tttgatagta ctttctcttc cgtgtcccag
     8821 tgccttttgg ccaaaataga aatgatttcc ttttttttca atcacattaa gggatagtcc
     8881 tgcatctatt gttgatcctt cctttgagac acccgaatca aacaaaatat ctatttctgc
     8941 ttccttgaaa ggcggagcaa ccttattttt tacaatcttg gtttttatcc tgattccata
     9001 catttctccc tgacttgttt tgagattctc aatccgtctg acttcaagcc tgacagaaga
     9061 atagaatttc aacgctcgtc cacctggggt cgtttcagga tttccaaaaa acacaccaat
     9121 tttttccctt acctgattaa tgaaaatagc agcagtctgg gatttgctga tgtagcccgt
     9181 cagttttctt aatgcctgtg acatcaatct tgcctgaagt cccatgacag agtctcccat
     9241 ttccccttcc agttccgctc ttggaacaag cgctgcaaca gaatcaatga cgacaacgtc
     9301 gattgcattg cttcgtatca gggactccgc gatttccagc gcctgttctc cagtatctgg
     9361 ctggcttatc aataattcat ccaggtttac tccaagtttt tgtgcgtaat taggattaag
     9421 ggcatgttct gcatcaataa atgctgcctg acctccattt ttttgtgcct cagcaattac
     9481 ctgtaacgcc agggttgttt ttcctgagga ttcctgtcca aaaatttcag taattcttcc
     9541 tcttggaatg cccccgacgc cgagcgcgat atcaagggaa attgctcctg tgggaatggc
     9601 gtcaacctca agtcgcgttt tttccccgag tttcatgata gaaccttttc cgaaattttt
     9661 ctcaatttgt tcaatcgcca ttgaaagggc tttttcttta tccatatcaa tttcctccta
     9721 tgggaatgtt tacgagttct ttatattctg gcccggtttg atgaagaatt gattcaaaaa
     9781 ttgtaatgtg gttaatctcc attcttcccc agtgttcatg ttgaaatttg gtttcaagat
     9841 cgtgtatgaa atttgaatcg attgccgaac gcgttcttcc aatagtgata tgggcatgaa
     9901 atttgttttc cgggggtaaa ccgatttttg ttgccgctct ttccagaata tcgtgaataa
     9961 gcaaaatagc aggatgttgt tcaacaccaa cccagatgac ccgtatgtga ttcctgtctg
    10021 ggaaaaaacc aataccctgc acatcaatgg aaaacggtat caaggaagta aatgcatttt
    10081 gaatgttgtg gagaaaatcc tgatattttt cttcgtgtat attgccaagg aatctaagtg
    10141 tgatatgcaa attttttgca gggacaattt ttattgcatt tttttgtttt gcaagcatca
    10201 cagtgattgg ttctaattca tcagtcaaga tgtccgttaa tttaattcct gaaaaaaccc
    10261 tcattgtttt ttcgtcagaa attcttcaag ccaattttta accacgtctt ttgctccgag
    10321 cccaaaagca agtgcaaatg cgagtgctcc gctggcaagg atgatgttaa aaacagacgt
    10381 gacgaaattt ccacctatac ccatttcatc aagagcaaga accactccaa aaatgataat
    10441 aacaaccttt gttgtttttc ccagtatata cgacttttgt attcctgcat ttcctgctgc
    10501 tgtattcaca accccttcaa cgatgttgcc cacaaggagt gaagcaataa aaaggataag
    10561 gccaatgatg attttaggaa taaacgaaag gaggttttca ataacttccg gaggaatggg
    10621 gacgcctgcc acattgatgg aaagaaaaac aaccacaata agaagcaacc agtagataac
    10681 acttgccatc aattccgaag gttcttttgt tatttggcct ctcagcaata tttccctgaa
    10741 tcccgaatcc tcaacaagtt tatcaatttt aagcatcttc agaaactttg tcgccaggat
    10801 tttgaaaacc ttagcaacaa tccatccaac gataaaaacc agcagtccga agagtaattt
    10861 tgaaatagca acgcccaatc tgctaatcag ctcttttgtt ggcgccagta gaaactcgtt
    10921 cattgatacc ctccgtttgt tacagccaca atttcgggtt caaccttatt ataattttat
    10981 tgtttctttt ttaaccaggg catcatactt ctcattttag ccccaacttc ttctatcaga
    11041 tgcttttcgg ctttttttgc aagtgcattg aatacaggcc taccaaccct gttttcaagt
    11101 atccactcgc gagcaaacac accatcctga acttctttca atattttttt catttcctca
    11161 cggacagatt catcaataat tcttggacct cttgtcatgt ctccatattc tgcagtatca
    11221 ctgatgacct ttcgcatacc ctgaatgcct cgttctataa tcaaatcaac aatgagtttc
    11281 agttcgtggc acgtttcata ataagcaatt tcaggttgat atcctgcttc cacaagtgtc
    11341 tcaaaggatg ctttgatgag ttctgtaacc ccgccgcaca gaacgacctg ctctccaaaa
    11401 agatcagttt ctgtttcttc ctgaaatgtg gtttcaacaa ctccgcatct cgtacctccg
    11461 agtgcttttg cgtaagcaag cgcgattttc aaagcattgc cagtgggatt ctgttcaacg
    11521 gcaacaaggc atggtacacc cttcccttct gtgaattgtg ttctcaccag atctccgggt
    11581 ccttttggtg caaccatgaa tatgtcaatg cttgaaggaa ctttaatctg actgtaaacg
    11641 attgaaaatc catgagcaaa accaagtgcc tggccatttc ttatgtttgt tttgatttca
    11701 ttttcgtata caacgggttg cagattatcc tgggttaaca tcatcatgat atctgctttc
    11761 tttaccagtt ctgcagtact tgcgggagta aaattatcct tttcagcaag gatccagttg
    11821 tttgttcctt tcaactctga taccattaca tttactccgc tgtctctcag gtttaacgcg
    11881 tgtgccctgc cctgactgcc ataaccaata attccaactg ttttgttttt cagaacactt
    11941 aaatcggcat ccttatcata ataaattttt gccatttttc tcctccacct gtttaaaaag
    12001 tgaattgttt atttttcttt tccggcttct ctgcagatgg caattcttcc tgttcttgtt
    12061 atttctttta caccgaagcc cttcataagg tctatcattg cgtcaacttt gggtttacta
    12121 ccaacaagtt ctattgtgag actttttctg cccacgtcga caattttagc gcggaaaata
    12181 ttcaccagtt gtacaatttc atttctaaca ttgctgtttg cagtgttgac tttgaggagc
    12241 acaagctccc tttcaatatg ttcctctgat gtcagatcct gaactttgat gacgtcaaca
    12301 agtttattaa gttgtttgta tatctgctca agtatttttt cgtctccact tacggcaatg
    12361 gacattcttg aaacagttgg gtcttctgtt tctgcaactg caagactatc aatattataa
    12421 ccacgagccg aaaacagtcc agcaattctc gcgagaacgc cggacctgtt ttccacagtg
    12481 acagaaatga tgtgttgatt tttttctatc atttatgcga gccctccaat catttctttt
    12541 aaacttgcac ctgctggcac catcggaaaa acattttctt ctggttctat atgacaatcc
    12601 acaaatacag ggccatcatt atgagaaaat gcctttgtaa gtatttttct taactcggct
    12661 ttttttgtaa ccctgtaacc tgtggctcca tacgcttctg caattttaac aaagtccggt
    12721 tgttttcctt tcaaacaggt gaaggcatat tttttattat agaaaagctc ctgccattgt
    12781 cttaccatac caagatggca attgtttaaa ataacaacct taaccgggat tttttcggaa
    12841 accgctgtgc tgagttcctg aatattcatc tggatactgc catctcccgc tatgtccacg
    12901 acgattttat ccctgcacgc cacctgtgca cctatcgctg cgggaaaccc aaatcccatg
    12961 gttcccagtc caccagagga gataaatgtt cttggatgtt tgaacctata gtattgtgcc
    13021 gcccacatct ggttctgtcc aacttctgtt gcgataattg cttcgccctg tgtgagttcg
    13081 gaaattgttt caaccacaaa ctgtggttta agaccatcag tatccttgta ggtaagagga
    13141 tatttttcct tccattgttg tatttttgca agccattctt ctatttttgt tttcgggcaa
    13201 ttcttaagca tctgagatag aacgttcttg acatctccaa caattggtat ttccaccagg
    13261 acatttttcc cgatagaggc agggtcaatg tcaatatgaa taattttcgc atgtggagcg
    13321 aattcactga tttttcctgt taccctatca tcaaacctgc atccaactgc tatcaggagg
    13381 tctgtctcca taatcgcatt attaccgtat cttgtgccgt gcatgccaac cattccaaga
    13441 tgaagtggat gttcggttgg gaaacttccc agtcccatta atgtcagggc aaccggaatg
    13501 tttgtttttt ctacaaattt tataagttgt tcagaagcat tggcgcttat cactccgccg
    13561 cccgcaagga tgagaggttg tctggatgtt tcaattaatg acaatgcgcg ttcaatctga
    13621 accggatgtc cattatacac gggtttataa cttcttattt caattgtttc aggataatca
    13681 aaatctactt ctcctctttg aacgtcaact ggaatatcta taagaaccgg tccgggcttt
    13741 ccagtttttg cgataaaaaa tgcttctttg aataccctgg taagatgttc ggtatttttt
    13801 acaagataat tgtgttttgt aattggtctg gaaataccgg ttgtatcaac ttcctgaaaa
    13861 gcatcattgc caatcaactg ggttgcaacc tgtcctgtaa ttgctactaa cggaattgaa
    13921 tccatatacg cggttgctat gcctgtaaca agatttgtgg cgcctggtcc tgacgtcgcg
    13981 agacatactc ccacttttcc agaagccctt gcataaccat ccgcagcatg cgcagcaccc
    14041 tgttcgtgtc ttgtgagaat aaatcggatt ccactatcat ataacgcatc acacagtggt
    14101 aatacaacgc ctcctggata accaaaaatc acctcgacct tttctttttt caagcattca
    14161 acaatgattt gggcgccttt catctatatc tcctatttac gtggctgtct acttcatctc
    14221 ttaaaaaaat tgcagtgcaa cgtgcctgct ggaggttaac tcaattataa gatgacgttc
    14281 atcaaatgtc aagaattttt gatgtaccct gtattgtata tgaaaaaaat tgggttctta
    14341 cctcgagatt atttgttctc gtgaagaacc gacggatatg gcagtgattc tggtattgac
    14401 aagatcctcg attctctcaa tatatttttt tgcgtttgtg ggcaaatgat ggaagtccgg
    14461 aacatcgttg atattttcat cccatccctg aaattcctca tattcaacct caatgttctc
    14521 aaatacattt gtatcaaggg gatatttatc aattggatta ccattatgat aatacttaat
    14581 accgatctta atcgttttta atcctgacag cacatcaagt tttgtgatga caagttcatc
    14641 aaggccattc aacattgcgg catattttac gatgacagca tcaaaccagc cgcatcttct
    14701 tggtcttcct gttgttgcac caaactcatt acctgctgtc ctcaactttt cattgagttc
    14761 gtccttgatt tctgtgggaa atggtcccat tccaaccctg gttgtatatg cttttgcgat
    14821 tccaatgatt tttgtgattt ttttagggct tattccaagg ccggtacatg ctccaccaga
    14881 aacaggattt gatgcagtga caaaaggata tgttccaaaa tcaatatcaa gaaatgttcc
    14941 ctgggctccc tctgccaaca ctgttttgcc attttcaagc gcatcattta agtacgcgga
    15001 agcatcgatg gtaagatttt tgagtttttc acggtatttg ctgtattctt ccaccagttg
    15061 ttcgactctt acggcggatt ctttatagta ctgctggaag agataatttt tcaactcaag
    15121 ggcgatgtta agttttttta caaatacttc ttcgttcaaa aaatcaacga ctcttactcc
    15181 cattctccca tatttatcgg tgtatgtggt accgataccc ctgcctgtgg ttcccaattt
    15241 gtctttaccc cttttgttgt cctcgatttg atcaaggatt ttatgatacg gtaaagttaa
    15301 atgggcgttt tctgcgatga agagacgatt ggtgaaatct atgccatggt tttccaggtc
    15361 tgtcatttcc tgaaggaaag aggatggatc tatcaccact ccattgccga gaacccccac
    15421 tacattttta tgaagcagtc cagaaggtat cagatgaaaa acaaattttt gattattaca
    15481 gacaacagta tgacctgcat tcgctccacc ctggtatctt gcaacaatat catgctgtga
    15541 tgccagggta tctataattt tacccttgcc ttcgtctccc cactgagttc caacaaccac
    15601 tgttaccatt tatgccccca atttctttat aattgctttt cccatttcat cggtgcttac
    15661 ccctgctgta ccttcgggtc tgaaatcgta ggttgtgtgt tttccttctt caatgacttc
    15721 cgataatgca ttctcgagtt tttcagcgga ttccacctca cctatatact ggagcatcaa
    15781 aacagccgcc agtatttgag ctgatggatt tacaacattc tttcctgcat atttgggtgc
    15841 gcttccatgt gttggttcaa aaatggcaac ctcatctccg taatttgctc ccggggaaag
    15901 tccaagtcca ccaacgaggc ctgcgcacaa atcagaaaga atatctccat atagatttgg
    15961 gcacgccagg acatcatatt tctgaggttt ttgaaccagt tgcattgcca tgttgtcaac
    16021 aatcctatct tcaaattcaa tgtcaggata attaagtgat actttccttg cgacatcaag
    16081 aaacagtcca tctgtaaact tcataatgtt tgctttatgc acagcagtga ctttttttct
    16141 tttatttttt ctcgcatatt caaaagcaaa tttcacaatt ttttctgtcc ctgtaattga
    16201 tatcggtttt atgctgatac ctgagtcatt gctgattttc ttgccgataa gattttcaat
    16261 ctctttgata actgaaagtg tttctgattt tcccatttca aattcaattc cggcgtaaag
    16321 gtcttccata ttttctctca caaccaccag gtcgatattt tcatacaggg ttcttgcacc
    16381 cctgtaaact ttgaatggtc tgacacatgc atagagattg aaaatctgtc tcaaagccac
    16441 attgatactt ctgtatccgc tacccaccgg tgtggttata ggtcctttca aacacacttt
    16501 atttttcttg atggactgga ttgtattttc tggaagcggg tctccgaatt tttcaatcgc
    16561 aggtataccg gcttcgcatt cttcccagtc aatgctaaca cccgttgctt tgatgcactc
    16621 tttcgtaact tctgtcaatt ctgggcctat accatcgccc gggattaatg tgactgtatg
    16681 ttttttcata tttgaaagtc cttgtatttt ttgagataat taatgatacc accctcattg
    16741 ataattttga gcaagaattc cggtaagggt ttaactggaa catggatatt ctgggactcg
    16801 ttaaaaatat gccccttcag aagatctatt tgcagaatat catgttgtga aatcggatct
    16861 gtattggctt ctatcaaaac aagccctaca tttattccgt ttctaaagaa aattctggca
    16921 aaacttttcg cgataattgc tccaattttc gcatatttta aagcaagcac tgcttgttcg
    16981 cgagaagaac cgcatccaaa attattacct gccacaataa aatctcctgg gttaatcttt
    17041 tttgaaaaat caggatcaat gtcttcaaat atatgagatg caagttcagt catatccagc
    17101 gttttgaatt tgtatcgccc tgaaataata taatcagtat taatgtcatc tccgaattta
    17161 tgcacctttc ctgttaatat cataatttcc tccttccaaa caaagtattt tacaagaaat
    17221 tgagtacaag gtcaaacgtg tgaattgtta tcatgctttt gctactgtca atacctttac
    17281 ttctctggct ccattttttt tcagggtttt atatacttct tcgatagtgg cgcctgttgt
    17341 gtagacatcg tcaataacaa gtattttttt atccttaatg atggaagtat tccttacttt
    17401 atatacattt gcaacatttt ctttcctttc attgatatca agatttgttt gtgagcgatt
    17461 attttttgtt ttcacgagtg cgtggtaaac agcaggtttg ttttgcattt taccaagatg
    17521 aaatgctatc agccagcttt gattaaaaga tcttttgaat tctttcactg gatgtagcgg
    17581 tactggaata attatatcga atttaatttc tgaatgttga gtaataaaat cgttcatcca
    17641 cgtggcaaag gaaaatgcaa tccattttct ttttttatat ttatatgtgt gaatcgcatt
    17701 catcaccggg ccttcgtaga gagcaagcga aaatattcgt tcgtgtaacg atggagcaat
    17761 tgtaatatgg tttagacaat caatacagaa gcaatatttt ttaattgcga cctctcgtcc
    17821 gcacccacag catttttctg gaaaaatcgg gtctatgata tgatgaaata ataagaattt
    17881 caatttttgc aaaaccatga gattacaaaa aaaatatatt tatacggaat ttttcaattt
    17941 ttatgtcatt gcctttttta tatttgcctt catttttatg atgagggcac ttttattgct
    18001 atcggattta attataaacc atttttttac tcctgtaact gttgtcagat tttttctctt
    18061 ctctttcttt gagatatgtc ctgatattgt ttctctttcc tttttaacgg ctgtaacggg
    18121 tactgtggca agaatgaata tggagagaga atactgggtt tttttgacgt caggggtttc
    18181 ggcgaaccag attgttagat attttttatt gcttggagtg atttttattt ttcttgaaat
    18241 atggttatgc ctttttttgt ctccgaattc aacatatgga aagaaattgt tactgaatca
    18301 ggctcatctt gacgaaccca tgaagttatt cagaaatcaa acattggtca aagattttcc
    18361 cggattgacc atttacgtgg aaagggttat gaacagaaat ttttacaatg tcgtaatttg
    18421 ttacagggag ggttctgatt ccgtatgtat catcaaggca gaaaaggcgc atttaagaat
    18481 agacagggat ggatttctgt gcctttttct tgagaacggc ttccttgaga cgcattctat
    18541 aaaaaaagcc accttacttg taaaagtgga ttttgatgtt tacacatttt ctcttcctta
    18601 taaaagaatt gttgatgctt ttcctcagag aaaaatcaag gaaatgaatt ttttcatgct
    18661 tttgaactca attgatgtta gaacaaacag aaatgaaatt ctggtgatgc ttttcaagaa
    18721 gattttcttt gcgactttac ccctgtttta tgtgttcatg gggttctata cgggaatagg
    18781 aatcagggcg acaggatatt tgcagatact tggaattggc ttatttatag gacttggttc
    18841 ttatttcatg attcttgttg gcgagggtat cgcattcaaa accaatagca gttttatgtt
    18901 ttttgtaaca cccctaattt ttatattcgc cattattgct gtaaaaagga aattttcaca
    18961 tgttgcttag agagaaacat atcttgctgg atactttcaa attttttctt cttttgctta
    19021 cggcttttgc aatactatta tttattttgc aaatattctg gaccattgca gatgcaagac
    19081 atacgagggt ttttttatgg aaagattgtg tggtgaattt ttatgatagt tttcttgtag
    19141 taattccttt tattaccata ttaagtgctt ttttggtttt ttctgaaatg gaaaaattca
    19201 agcagatttc catttttgaa ttaaaaggaa tttctgaact tcagatattt aaaatatttg
    19261 ttatttttgg ttttttgtgt tctttgtttt cctttattgc ggggttgctt acaccctatc
    19321 agaacgaaat aaaaaataac agtaggaaga cggattcatt gagtattctt acagaaagtg
    19381 tatgtttctg ggccacggat tataagcaac aggatattgt ggaaaatctt attatttcgg
    19441 aaaaccgttc taatgtaaca tattatgcaa agaaggcaaa attcttcgaa aaaagcatta
    19501 tactttatga cggaaaaatt gtttatgggg atagaacaac acgacatttt caacaattta
    19561 ctttagaaac tgatttttgt ccccgtgcgc tgcttttata tgcggttggt tttcctgaaa
    19621 gatggacgtt tttcaagtta catggattat tgaaacaagt tgcatcagtg ggtatcaaat
    19681 caaaatctga ctggattttt ctttattcaa aaatatccta tcccatgttg aacattttta
    19741 tcattttact tctattcccg tttttccatt atagaaggtc tatggcaaaa accaggattt
    19801 ttagtgttgc tttcacaggg acgctgatgg tgtattcatt atattctttc ggtctttctc
    19861 ttggcagagc agaaatcatt ccctggcagg tcgcgccctg ggtatcacat ttgattttaa
    19921 cgattttttt catcctttat ttgtcagcgg taaggaaaaa gatgtataat tttatttaat
    19981 tttgtgcaat gcttattgct tctggagaac gatatgaaaa cagaacacct tttaatagag
    20041 ataggatgtg aagatttacc ttcctggtca ggacagcatt ttataagcag gttcaagccc
    20101 ttatttcttg gcgaattgaa taattacaaa ttgactgcgg atgatgtgat gttttttttc
    20161 acccctcgta gatttgttat tttcattaaa ggtctgtctt caaaaattcc tcccgaacgc
    20221 agagaaatta caggtcccag atatgaggct gcttttgata aagatggaca agcaaccatt
    20281 gctgctgctg gttttgcaaa agcacacggg gtgtctgttt cgtctctgaa agtgaagaat
    20341 gtgaatggta aaaaggttgt gtatgtgaca aagacaatac caggtgttgc tacaaaggat
    20401 attattccag atattcttgt tcgcacactt agaaaaattg atattccgaa aggaatgagg
    20461 tggaatgaat cacaggaagt gttttataga cccgtgcgat ggatgcttgc aatatttggg
    20521 aaccagaatc tggacgtcaa ttttggcggt ataaaaagtt caagatacac ttatgggcac
    20581 agaattctgc gtccgaacag aataagggtt cagaactggg aagattattt tgacaaaatt
    20641 cagaaatctt ttgttcttct gggtgaagat ttaagacaac aatttatact caatctcatc
    20701 agtacgaacc tcaaagaaaa tgaaaatttt gaagaatcca tgggcaagga ccttgtaaat
    20761 cttatagaat atccacgcgt gatacggtgt aattttccat caattgatta caatctgcca
    20821 gaagaggttt taagggttct gatagcaaag gcaaaaggta ttccaatttt ttgtggtgag
    20881 caactgaaaa aagaatattt tgttatcagt gacggtaatg ataacgaaaa tgtacgagtt
    20941 aattatgaaa acctgttgaa aacaagaatt ctcgatgctc agttctttta taattcagat
    21001 atatctgttc cttttgatga attcgggaaa aaactggata aaattgtttt tcatcaaaaa
    21061 tggggttctg tcgcgaaaaa ggtcgagagg ttgaaaggac tggcgtttga attttctgaa
    21121 ataatggggc ttgattcaga acagaaaaaa accttgattc ggtcggctga aatttgcaaa
    21181 aatgatcttg cttcagaaat ggtgagggaa tttccggact tgcatggaac tatgggagca
    21241 atatatgcaa aactttccgg ggaaagtgaa gatgtgtgca gaacgattgc agaatacatg
    21301 cttccagcat cccctggaga caaacttcct caaggtatat gtggtattct tctgggcatt
    21361 attgatagac ttgattatgt cgttgccttt atatccgcag gagttgatgt ttctggttca
    21421 gaagacccct atggtttgaa aagaattgcc ggaggactgt tccccttgat ttatgtgtct
    21481 ggctttgagt atgattatcg ccaaattgtt agaaagatac ttgaatatta tgacattcct
    21541 gctgaagaaa aaaacagtat ttcttcaaaa attgatgaat ttcttataca gagatatgaa
    21601 tctcatcttg aatctgaggg ttttccgaga ggtttaagaa gaagtgtgtt gactatcgat
    21661 ggcatgaatt ttctaaaaat cagaaaaaaa ctggaggcac tgaagaattt catcaaaaac
    21721 gatattgatg cagaacagat attgattcct gttacccgta ttgccaatat tttgagacag
    21781 gcgacagaaa ggaaaattca gattcctgag ttccatcagg atttgattga ggaatctggc
    21841 gaacgaattc ttgcagaagt ttacaatact tacgcagaaa aatctgaagg attgcttgca
    21901 caaatggatt atgataattt cttaaagatt ttatcaggtt tgaaggagcc agtggataat
    21961 tttttcgata gcgttcttgt tatgtgtccc caggaggttt tgaggaataa ccgtcttgct
    22021 cttttgaaaa aatttaatga tgtttttctt aaattcgcag acttttcata tatcagggaa
    22081 gaggatataa aaaatgctca aaaatactga ttcagatatt gccatgttga taaaacgcga
    22141 gatacaaaga gaaaaagaaa ccattaatct tatcgcttct gaaaattatg ttagcaaagc
    22201 ggttcttatg gcagtgggtt caattttgac aaacaagtat gcagaaggaa ccccatggaa
    22261 aagatactat gatggttgtg aaaacgttga tgaaattgaa agtattgcca tagctcgttt
    22321 gaaaaaactt ttcaatgctg aacatgcgaa tgttcaaccg catgcgggaa gtcaggcaaa
    22381 tatggcagtt tattttgctg cattgaagcc aggggatgta atactcggga tggaccttgc
    22441 tgctggaggg caccttacgc atgggacgaa ggtcaatttt tcaggaatga tatttaaatc
    22501 attttactat ggagttgacc gggaatcaga acgacttaac tatgatgaga ttttaagcat
    22561 tgccaaaaaa gtaaggccaa aattgattat gtgcggagca agcgcttatc caaggataat
    22621 tgattttgaa aaattttcag acattgccag aaatgttgga gcgtacctgt gtgcagatat
    22681 tgctcatatt gcagggatta ttgctgcgaa attgcatcct tcgccggttg agtgttcaga
    22741 gtttgtaaca ggaagtactc ataaaaccct cagaggtcca agaggtggtt ttattttgtg
    22801 caaaaagggt tttgcgcagg aaattgataa ggcagtattt cctggcatac agggaggccc
    22861 tttgatgcat gtgattgcag gaaaagcagt ggcatttaaa gaagcaatgg ataaaaaatt
    22921 ttacttctat cagaaacagt tgcttgagaa ctgttcactg atggctaaag gactaattga
    22981 aaaaaaatat cgacttgtta ccggtggtac tgataaccat ttaatactga ttgatcttcg
    23041 taataaaatg ataacgggtg agcaggctca aaaaaccctt ggtatggtag gtattactgt
    23101 taatcgtaac gcaatacctt atgatccgtt gcctccgaat atcgcaagtg gtattcgtgt
    23161 tggcacagcc gcgatcacca caaggggaat gaaaaagggt gatattgaaa aaattcttga
    23221 tttcattgat gaggcgctgt actacagaga tgattgtaat aagctggcta tgattagaaa
    23281 acaggtaaaa aaattcgctt cacaatttcc catttatcct gaacaacgat gaaaaacaaa
    23341 ccaaggcccg gctgggatga gtattttatg aaaattgcgc acctggtgag cgaaaggtca
    23401 acctgtctca ggcgaaaagt tggcgcggtc ctggttaagg aaaaaagaat tcttgccacc
    23461 gggtacaatg gtgcgccttc tggtgtgact cactgtgaga aggcgggttg cctcagggaa
    23521 gaactgggta tcccatcagg agagcgtcac gaattgtgca gagggcttca tgccgaacag
    23581 aacgcaatta ttcaggcagc gtatcacggg gtaagcataa aaggcagtgt gctttatatt
    23641 acctgtcacc catgttcggt atgtgcaaaa atgattatca atgcgggtat aaaggaagta
    23701 gtaatttttg aaggatatcc tgataaactt gcgaagatta tgcttgaaga agcgtctatt
    23761 gtgataaggt atatcggaaa agatgtgagt aaacattgaa ttcggaggtt gatatgagca
    23821 agaatatttt actttttggg aaacaggatt gtggtttatg ccaggggtgg aaaaaaaaac
    23881 ttgagaattt caatattccc tttacatatc ttgatacaga aaatcctgat aacctgacgg
    23941 aatttacact gatggggctt acaaagatac cagcgctggt cattggcgaa aataagtttg
    24001 agggtattag gccatcggat ctttctatag atgaaattaa gaaaatgatg gaaaatgaat
    24061 aacaatgata cgatggttag aaatttgcca cctattattc tcagcgtttt gcatcaaagg
    24121 ggaataaaaa cccggaaaca aatacaggaa tttttggatc cttctgtttc ttctctttca
    24181 gatccatttt caatcagcga tatggaaaaa ggttgtaaaa ttcttgaaca agcaatttat
    24241 tcaaagaaaa atattttttt atatgcagac ggtgatgttg atggaattgc tggtgctgct
    24301 atgtttgtaa acctgttcag gaagatgaaa attccttttg acatcaaact tactcacaga
    24361 cttgaggatt atgaaattga gccggatttc ataaatagga taagaaatct tggttacgag
    24421 ttattgatta cgattgatac aggtacgact tcaaatgaac ttattgaata ctgtgaacaa
    24481 ttaggttttc cacttattat cattgaccat cataaaggag agatgttaaa aaacctgaaa
    24541 catgtggtta ttatcaatcc gtcactcggt agagaggatt ctgattttga tgtgttgacc
    24601 gcttcggggc tttctttcaa agtgattcag gctctcaaga gtttttcccc ttttttccct
    24661 gatgaaattt ttgcgtcgag tattgaactt gcgacaatcg ggactctcag tgactatggg
    24721 tttttaaccg gagaaaatcg tgtaatagtg aaaattggtt tgaacgggct tgagaatacg
    24781 agtattcctg gtattgaaac attcagggaa tttttttatg tacccggaaa gaacagtgat
    24841 gttgaatcta taacgcatta cctgaaccca aagttgaata cgcctggtcg ttttggaaga
    24901 cctgaactca ctttgaaagt tttgacagca aacaaagaag aagaaattca aaacgttttt
    24961 gaagaaatag aattttttga gaaagaaaaa cagaaaattc tcaggagatt ttcgtgttcc
    25021 atagaaaaat tgaaaaatcc tgaatgtccg ttgtttctgt tatttgaaaa cataccggcg
    25081 tctttttctg gcacatttgc cgcgaggaca agtgaaaaat ttcaactccc tgcccttgtg
    25141 gcgatcaaaa atggagattt tcttcagggt tcagcccgca gttacgatag agcagacctt
    25201 tatgaacttt ttaataattt caagaactat tttatttcct ttggtggtca caaaaacgcg
    25261 gttggtttca aaatgaaaat tgattttcta aatgaactta aacatgcatg gaaacagtta
    25321 aggctttgta gtatttcttc aaaacaatct gtgaaacctt tagaagcaga ttttgaagcg
    25381 ttgacgattc ctgtgtttga gaatattcaa caattaaaac catttggacc tggaaacccg
    25441 ccggtacttt ttatttcgaa tcccgttgaa tgtttgaaaa ttaccggatg caaaggtaaa
    25501 aagacaattg catgggttaa acaaggtaat aaaatgtttg aagtgcattt ttcaaaaggt
    25561 ttcaagatgc caggaggtcc tatttcaatc acatatgtgc cacatttgag aaaatcagaa
    25621 ggactttata ttccctggct ggatgtaaaa aattattcaa cgaccttttg aggccttttc
    25681 acttttcctg atttcagaca ctgtgtacat atagccaatc gtcttgtttt cccctggtat
    25741 tcaacaagca atttctgcac attgggtttc catgttcttc ttgttttaac atttgaatga
    25801 ctgacattat tgcctgtttt ttgccctttg ccacatattt cgcacttcat ttctactcct
    25861 ttttatctgt ttttagtttc gtttgttact atatttttca attcaggtat gcttttcaac
    25921 aggtattgca catactcatg ttttggattt atcgacataa ctttttccag gtattccttg
    25981 gctcccttaa tatctttttc ttgaaatatt ttaatggaag cgagtccaag caatacatcc
    26041 acatcatatg gattaaaccc taacgccatt tcataataga cctttgccct tttaaaattc
    26101 tgtgtttgca tataaagtgt accaagattg gcatctgttt ttgcgaaatg cggaaaaata
    26161 ttattattga tttttaaata aacttcttct gcatttttta tatcgccaat ttgtccatac
    26221 gcataagcca ttttatacaa ggctaccgca ttgtaaggat ttattttaca cactttttta
    26281 taattttcaa ttgctgtaat aaaattgttc gcttctcttg ctgctattgc tttttttaaa
    26341 taaacctcag gaacgagccg ataaaaggtt ttccaccctg ccagggtaat catggcaatc
    26401 attactattg cagcagtggt ttttttcaag aagccagata tttgaatggt ggtggttttt
    26461 tttaaactga aacacaatcc gagaaggaac cagaaataca ttgcggtaga agtgtttgtg
    26521 aggttggtgg aaaggatatt gtcaacaagt acagcgatta ttccagatga ggcaaaaaga
    26581 aaagtattat ttgattcttc atcttttgtt ttcagtccgt tgtaaattgc aactactatt
    26641 ataataaaga aaaatacaag tccgataatg ccattttccg accatatttc gagatactgg
    26701 caatgtggat gattggttat tggagtcgcc tgtggtctga gaaaatattc tctgaaacgg
    26761 aaataaggat aataaaatat gaaattacct gtcccccaac ctgtcacgat tttcgctttg
    26821 attagattcc atgtaccttt ccatatgtaa tacctgatat cctgtacaaa ttgagtctgt
    26881 attttatgcc acataatggc tgttccaaaa acaataataa caaaaccaat gtatttcagc
    26941 cagggaattt tatgatttct taaaagaaaa aatgaggtaa aagaacaagc tgtggcaagg
    27001 gcaaaaagag atgctcttga ttttgtaaga atcagggcgg caagcgttat gatgaaataa
    27061 atggctgcta tggtattgag tttctttttg aaaaaataaa gggtgaggaa cacaggcatc
    27121 aataaatgcc ctgcaaaaaa attcggattc ccaaaagttg agatgggata atgtgttttt
    27181 ggatactgaa caaaagcagt gattgaggcg attaccattg ctgaagccca tatgaaggcg
    27241 tctttttttt cccatttcca taacataccc attatgaaca acaggtagta cagaagataa
    27301 ttttcaagtg cctgggctgc tccatatttt acaggtgcat aaaagcatcc cataagaata
    27361 aacgcaaaaa ataatgtacc agcaagaaaa cctgtaaaat ggatagaaat cgtttctttt
    27421 cttaaaaaaa gcgccgcagc gaaaattgcc accgacaacg cgccaaggat atcttttatt
    27481 atggtataat cgtaggcagg tttaaaattt aaaagcggtg caattccaaa aaataatata
    27541 gaaacaatag taaccaatgt tctaaaccct atcgtttctt catctttttt taacatctgg
    27601 ctgatgtttt cctgttcttt tttctttttt gacattgtgc ccttttatgt tatttgtccc
    27661 tgaaaaacaa aaatccagtg aatattatta tagcaaaatc gagccatata ctccagtttt
    27721 ccgcataata cagatcatat ctgaaccttt cttcgggttc tacctgacct cctttcagcc
    27781 catgaatctg agcccatcct gtgatgccag gtagaatttt catccgttcg taaaatccag
    27841 gaatcgaaaa aaacaatctg tcttctgttg ataatggtct gggtcctaca atactcatat
    27901 ctgatttgag tacattaaat aattgtggaa gttcatcgat gttagatttt ctcagccatg
    27961 tgccaatttt tgtgattctc gaatcctttt caagagtata tggaaggata ttttctgcac
    28021 ccttcaccat tgttctgaat ttgaaaattt tgaatttttt atttctgtat cctgttcttt
    28081 cctgaatgaa aaaaaccggt ccatcagaac tgaatttaat aatagttgcg attaaaaaaa
    28141 gaaagggcat gcttattgtc agaaatgcca tgctaaaaag aatatcaaaa aatcttttta
    28201 taaccttatt atgccagatc tcaagtggca aaatatcagt tgtgataaca gggattccat
    28261 atatttcctc cacccttccg ccgctgagaa aaactcttga tgttctggga taaacataat
    28321 attgaatgcc tgaattgctg catatacgtg ctatctgttg aattttttga aaatcttcaa
    28381 ggcaaatgat gacacgaata ttgtccggtc tgtccgaatt ttgtacagaa aattgcagtt
    28441 gttcaacatg ctggaatata attctatttc ctggaatacc gtgaagtttt aacctcttta
    28501 caattggagt catgtctctg ttatctccaa cgagatagaa ggtgaaatct tcttttgcca
    28561 gggttacatt tatgatgaga tgtaccaggg atagaataaa aaacgagata aggataccga
    28621 gtatgattgc aatccgtgaa taataaaatc ctctataaaa aaatgtgagt gcactgataa
    28681 taatggcaga gtaaaaactg ctttgaataa tcttactcag ctcaaaacat aaattgataa
    28741 atttgtgctc tttataaatt ctgttgacat tgagcataac aatccagatc aacgctgcaa
    28801 aaatggatat gtgaatatat ggtttaaggg gagcgatccc tttatctgga aaaatatgcc
    28861 atgaaaatct tattgcatat ccaagtaaca tgcttattaa tataataatg aagtcgagaa
    28921 aaattaaaat ttttttatcc attgttttcc acagagatta ataactgttc atatttctga
    28981 attacatcat cccatctgta gaattgtttt actctttccc gtgctttgtc tccaagggat
    29041 gaaaattttt ctggactttc aaccatttgt tttatcaaat ttgccaggga gccagatttt
    29101 tttgtgaaat aaagtccgca atcaccaagg acttccctgt tgaatggtac gtcaagcgcg
    29161 agaatcagtg ctccgcaacc catggcagat aggagcgcag gattggttcc accaacttca
    29221 tttccatgga tatatgcgag tgaatttgcc tgtaactcaa gatatgcctt tttttcatat
    29281 acgggcccgg taaattttat ctgctgggaa gcaatattaa gaatgttttt tacataagaa
    29341 tttttctttc tgatatcgcc ggagatagcc agaacattgt taatatttgc taattgaaat
    29401 tcttttatca tcaattcctg attattttct ggctcaagcc ggctgccatt aaaaaaatat
    29461 gtttttggct gcaatcccat tgatttaata atttctggcg acttagaata aaagatgtca
    29521 gcaccatatg gtatatagac acttttcttg tgaaatcggg aatcataata ttctgcgatc
    29581 ttgtgggagt cagcgacgat tgaatcagca atgaaagtag aaaaacagga agcaaactta
    29641 aaataggtct ttccgcaaaa tccccatttt tgcctcttcc attcaaatcc atcaacgttg
    29701 atgacaaatt tttttccata aattttccca aaaattgcca agggactatt ggctgcgtta
    29761 aatagaagta ttacatctgg agaccatata attgttttca acatgctgaa aaaagtatga
    29821 gaaaacgttt ctgtgatttt gcccctgata caaaagggaa aacaaacctg tatattgtcg
    29881 ccaggcaaag gcttgtcagg gtaaagatat tttcgacagg gtacaaaaat tttgtgtcct
    29941 tttttaccga gatactctgc aatcttttca gcgcaggttt caaagccacc gtatctggct
    30001 ggtattcctc ttattccaag tatagcgatt ttcatgttta tcattataac agggaaacaa
    30061 gatttctgta tacttcaact gtttttttcg cgcattccat ccacgaaaat ttttttgcct
    30121 gtttgagccc tttttctttt aactctttct ggagattgcc gttttgatgc aatgttaata
    30181 ttgctgacga tatctcctgg ctattatatg ggtcgaccag gatacctgcc tcttccacta
    30241 tttctggtag agaagaactg gcagatgcaa ttaccgggca accgcatgcc attgcttcca
    30301 gaacaggaag tccaaaaccc tcgtaaaatg atggataaac cagcatttct gcaccactgt
    30361 atagagcgat gaggtcgtcg ttttcaacat aatctgtaaa caaaattttt tctttaattg
    30421 ttggttcccc tgcagaaaaa atttcatcat ataaccataa ttttttccct gtaataacaa
    30481 gacaacatgg aaaatcttct ttttttttca gtatcaggga ataggcactt atcaaaccct
    30541 gaagattttt tcttggttgg agatttgaaa cgctaatgat ataaggacgg aaaattttat
    30601 gttttgtaag cacttgttgt atcttttctt cgtttacagg tttgaattct tcagaaaccc
    30661 cattataagt tatacaaact ttttcatcaa tatcaccata tttttttaaa atttctctct
    30721 ttgagaactg agaaaccgtg atgatttttt ttgcctttct tattgaggac ggcataagtg
    30781 ttttcatttt tatccgttca aaaaaaggaa aaaattcagg atgaatgaga taagaaacat
    30841 cgtgtaacgt gataaccgat ggaatctgga caaaaggtgg aatatgatac tgggtatgta
    30901 agacatcaat gtttgtcctt tttatttttg atggcaggtc aagcatcaat cttttgattg
    30961 aagatgtgga gaccttttca aatgagaaag aaggatgtcc tttcagaaat gaaggtattt
    31021 cagccatgtg gttcaggaaa acaataaatt gtaaatccgg aggatttaat ttcgaaagcc
    31081 ctctcaagag cccttcgatg tatctttcat ttcctccctg tttcattcca attgcatggg
    31141 cttcaatacc tatttttatc atgtttattt cttgtttttt cgataagatt gttgtacacc
    31201 tcaagatatt gcatggcagt gtgcttaata tcaaaatgat
//
LOCUS       DAORYU010000002        17838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_2598_length_17838_cov_4.554181, whole genome shotgun sequence.
ACCESSION   DAORYU010000002 DAORYU010000000
VERSION     DAORYU010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 17838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17838
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2598_length_17838_cov_4.554181"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..1194)
                     /gene="purM"
                     /locus_tag="PLW07_00155"
     CDS             complement(148..1194)
                     /gene="purM"
                     /locus_tag="PLW07_00155"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOL48969.1"
                     /translation="MITYKKSGVNDQKAEMFVSLIKERVKQASFSRKMSGDIGFFGAF
                     IPVSTGNKKSFLVSSCDGVGTKILVANAFEKYDTIGIDLVAMNCNDVAVCGAKPLLFL
                     DYIAVGKLKIEREQKIIEGIIKGCEIAQCVLAGGETAQMPDVYSEDDFDLAGFCAGMV
                     EADKILGPLRVKNDDVLLGITSNGLHSNGFSLVRKVLLSGRGMKRMLAAYQISLGRSM
                     GEELLRPTYIYSTVLSALAENRLIHAAAHITGGGIPGNLVRILPEHLDAEVYLNSWQI
                     PQIFHMIQSAGKVMQKEMFNVFNMGIGLILALPERNVDRVKSIVQKHGFRSFLIGCIC
                     PGKGRVRLLKKSAG"
     gene            complement(1303..3177)
                     /gene="dnaK"
                     /locus_tag="PLW07_00160"
     CDS             complement(1303..3177)
                     /gene="dnaK"
                     /locus_tag="PLW07_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOL48970.1"
                     /translation="MEKIIGIDLGTSNSAAAIMEGGKIVVIPSAEGTTIVGGKAFPSY
                     VAFTKDGQILVGEPARRQAAVNPEGTIFGAKRKMGTDFKYTVNGKTYTPQQISAFILQ
                     KIKKDTENFLGYPINKAVITVPAYFNDNQRQATKDAGTIAGLEVVRLINEPTAAAFAY
                     GLDKLNQELKIMVFDLGAGTLDVTIMEMMGGGVFKVLSTSGDTQLGGADMDQDIVDFI
                     VKDFQMQTGIDLINDRMAMQRIREAAEKAKIELSSTLETEINLPFITANDKGPQHLSM
                     KIRRAQLEELVRARVEKCRAPMEQALADAKLTPSQIDKIILVGGPTRMPIIQKFIEDF
                     MGKKPERGVDPMECVATGAAIQGAILAGDLKDKDILLLDVTPLSLGVETLGGVFTRII
                     ERNTTIPTKKSQIFTTAADNQTSVEVHVLQGERPMAAHNTSLGRFHLDGIPSAPRGVP
                     QIEVTFDIDSNGILSVTAKDLATKKEQSIRITASKKLTKEEIDKMVREAEQFAEQDRK
                     QKELIELCNQADGLAYSVEKTLKEHESKIPEAERNTAREKIAALRKAIETKNENEIRT
                     LMQEVEKSSHQIAEILYKQTSQSGQTDQQTTAQTSEPQSEQKDKKVVDADYREVDGNK
                     "
     gene            complement(3314..3387)
                     /locus_tag="PLW07_00165"
     tRNA            complement(3314..3387)
                     /locus_tag="PLW07_00165"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3351..3353),aa:Val,seq:tac)
     gene            3498..4127
                     /locus_tag="PLW07_00170"
     CDS             3498..4127
                     /locus_tag="PLW07_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HOL48971.1"
                     /translation="MLEDIKQAIIRGDAKKATELVSKALAENIAVEKILNEGLIGGMN
                     VVGMKFKNNEFYVPEVLIAARAMNQAMAILQPEIAKAGIKSQGKVAIGTVKGDLHDIG
                     KNLVSMMWKGAGFEVMDLGIDVPEEKFVEAAKNGAQLIGLSALLTTTMPAMKDVIEKL
                     KSEGIRDKVKIIIGGAPVTQAYADEIGADGYSPDAGSAVDKAKELLGLQ"
     gene            4142..4753
                     /gene="plsY"
                     /locus_tag="PLW07_00175"
     CDS             4142..4753
                     /gene="plsY"
                     /locus_tag="PLW07_00175"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOL48972.1"
                     /translation="MSFFSTIYWIILGYLSGSLVWGYWIGKFFYGIDLRNKGSGNIGA
                     TNVFRVLGPVPGIFTFVLDVLKGALPVILVKNIYAKDANQIILATVGIAAIIGSKFSL
                     FLRGKGGKSVNCSFGVILAMMPVEAIFSLVVWLGIFTLTGYVSLASIAAATALPFLIL
                     ILQKNMPLIFIGILIFIVVIFAHRDNIKRLIEGKENRFSLWKK"
     gene            4741..5718
                     /locus_tag="PLW07_00180"
     CDS             4741..5718
                     /locus_tag="PLW07_00180"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOL48973.1"
                     /translation="MEKIGIVGAGGWGTALSIILSDKFEITLWEKFPAYATVLSDTRE
                     NIEFLPGFKIPEHVAITSDIQTVCNNSFLVIVVPSQYFRETIKNVKPFYKSQKVLIAT
                     KGIEPETGMKMSEILYNEIGQCKFAVISGPTIASEVAAGKPTAAVVASGDTEMAREIQ
                     CMFSSRILRLYTSDDVCGVEICGGYKNVIAIGSGIIDALELGDNAKASYLTRSIEEMK
                     RFGKMLGAKEKTFLGLAGIGDLITTSFSPSSRNHRFGQAIATGNAQQFLAHTRMVIEG
                     ISASKAIIKMAETHNVDIPIASSIYRIVHKNAKPSDEIEKLMTRELKHE"
     gene            5711..6037
                     /locus_tag="PLW07_00185"
     CDS             5711..6037
                     /locus_tag="PLW07_00185"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOL48974.1"
                     /translation="MNDSKRYYSINEVSEITGLGTHILRYWEKQFSFLKIQRDPSGRR
                     IYSDKDVEKIKEIQKLLYQNGYKIVGVKKKFRSISYTQKQSRQNSSLLLKKILRYIKE
                     IEKCLP"
     gene            6025..6639
                     /gene="purN"
                     /locus_tag="PLW07_00190"
     CDS             6025..6639
                     /gene="purN"
                     /locus_tag="PLW07_00190"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOL48975.1"
                     /translation="MFTIGVLASGKGSNLKSIIDQIKIGFLNVRIGVVISDNPQAYAL
                     EITRTEKIPGIYIEPGKHKTFLDPAQEKKYIQCLKEHHVELVCLAGFMRVIKKEFFNA
                     YRGKIINIHPSLLPSFRGLEACKQALEYGVKFTGCTVHFVEEDIDTGPIILQAIVPVL
                     GDDSPESLHKRIQEKEHIIYPVAIKLISENKLVVQGRRVMIHED"
     gene            6629..7090
                     /locus_tag="PLW07_00195"
     CDS             6629..7090
                     /locus_tag="PLW07_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012963025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOL48976.1"
                     /translation="MKINIRPAVIDDAYSIKNLINMHAKKGKMLPVSINQIFENLRNF
                     WVLCDSKGKILGCACLKIVWKDLGEIRSLAIKNSKQKKGYGSVLIETLMKEAEKIGIR
                     KIFVLTYVPEFFEKFGFVRIEKTKLPHKIWIDCINCPKFPRCDEIPMLKKL"
     gene            7087..8178
                     /locus_tag="PLW07_00200"
     CDS             7087..8178
                     /locus_tag="PLW07_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL48977.1"
                     /translation="MKKFLLVLFCLYGFQIHAMADTPNTQGLEDFLSGRDLKMQRSFM
                     NNMIATGKNLEFSGISYEDIFLFGITINFSGTSGKDVYITGDTVKISGKIRGNLKVMA
                     RNLIVENCEIDGNVNILSPEILMADSVKCKNTTKIWSRNVQMGGQYKTVNIKTRDIIF
                     TKNIDVKNLFVQSQTKPSLPVNMLKHCNFQYEPPVPQKAQVFFSSKFRKLYSFLSLCF
                     PFLLMTIMTPKVLQETIEMIEKKPVWIFFSGILLLLIAPLILILLMVTVIGAPIGLIL
                     LTFYISLLYLCRGFTCVALGRILLFKLKESKMKLILAIILGTGVFVLLTAIPKASVIV
                     QIPFVIFGFGGFAIGRIRMFLTLRKQNLI"
     gene            8193..9395
                     /locus_tag="PLW07_00205"
     CDS             8193..9395
                     /locus_tag="PLW07_00205"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOL48978.1"
                     /translation="MKKIVVAYSGGLDTSVIIKWLQTKYNAEIIAFTADIGQKENPSL
                     LEKKALQTGAKKFYFMDLKDEFLSDYIFPSLKGSALYEGKYPLATALSRPLIAKNMVD
                     VAKKENADAFAHGCTGKGNDQVRFELTFKNLAPEKEIIAPVREWEFKSREEEIEYAKK
                     HNIPVSVTKKKPYSIDWNLWGISIECGILEDPWNQPPEDAYQITQNPEKAPSKPEYIE
                     IEFYKGIPTGINGRHVSGLKLISILNEIGGKHGVGRIDMVENRLVGIKSREIYEAPAA
                     VILHAAHSELEKLILERELLHFKTIVSQKYSELIYYGYWFSDFRICLERFIDETQKYV
                     TGTIRVKLFKGQCTVVGRKSSFSLYEEKLSTYTEKDIFDHRASKGFIDIYGLPLILEG
                     RRNRKKAR"
     gene            9397..10293
                     /locus_tag="PLW07_00210"
     CDS             9397..10293
                     /locus_tag="PLW07_00210"
                     /inference="COORDINATES: protein motif:HMM:NF012795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOL48979.1"
                     /translation="MEEEKIIKWLKSHCLPERTEVKTGIGDDTAVIDFNSTSYLLLTT
                     DAVVENVHFKINDASFYQIGWKALGVNISDIAAMGGSPLWALVSIGMPKKIVSAYKKL
                     YKGILDMSKLFHVDIIGGNLSRSEILFVDIFLAGTVKKKNLVLRSTAKPGDILFVTGS
                     LGGSQKRKQFTFIPRVREAQAIISRVKPSAMIDLSDGLAADARKLAEASGCGFEIAIS
                     KIPVSQDACEKDKIVSALYDGEDYELLLAIPPESVRSLPTIVNGIPVTEIGRLTKKKT
                     FVLKHTNGSTNKISKDKFRHFE"
     gene            10244..10723
                     /gene="tsaE"
                     /locus_tag="PLW07_00215"
     CDS             10244..10723
                     /gene="tsaE"
                     /locus_tag="PLW07_00215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOL48980.1"
                     /translation="MEAQTKYQKINSDTLNDKKKILIFETDSAEKTEKLGSKIAGLLK
                     KRKIHCVVFTGPFGSGKTTMIRGIIKKLTGKSHVTSPSFTMVHEYREDNISVFHCDFY
                     RIKNPDELESIGFREYENGFLLIEWPESAFEKIPENSLKITLDFVGLKRRKIIVALP"
     gene            10720..11685
                     /locus_tag="PLW07_00220"
     CDS             10720..11685
                     /locus_tag="PLW07_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOL48981.1"
                     /translation="MKKLILKRLLQLIPMLIGMSFISFIIIQSSPGDYFAQMKMNPEI
                     SPETIEMMRTSFGLDRPLFVQYFFWLKNICKMDFGISFAYHIPVTTLIKFRLKNTLCL
                     AIFSLFLSWVIAIPLAVLAGYKEGTLIDKIFSLFAYLSISLPSFFVALLFVYFISKTG
                     VLPLGGTRSIFDIDANAFLIFKDYMRHLLVPGLVLCFVNIGWLFRLMKNNFIEVYNTP
                     YILAAWARGLPASSVIFKHTLRNALNPMFTILGMEIGSILNGAALTEIICGWPGMGSL
                     ILEAVLSHDLYLVMGSLVISGFLLIIGNLIGDILIALFDPRIRLT"
     gene            11682..12740
                     /locus_tag="PLW07_00225"
     CDS             11682..12740
                     /locus_tag="PLW07_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012674697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOL48982.1"
                     /translation="MKIFFQRLKNHSKLAYFSFWFLIFFYIVSFLADLLAPYHFDHQF
                     RNKPYHPPTKIHFVDENGHFHIRPFVYNMVVDDPVFRTYREDKSIRYPVKFFVDGPER
                     KIFFIFKTTRYFFGVDEPAGIFLFGTDTYGRDIFSRLLYGGRISLSISIVGVLISFTL
                     GMITGGIAGYFGGLLDQILMRFVEILMVFPSFYLMLSLRAMFPLNLSSIQVYFLIVLI
                     LSLIGWAGLARIIRGMVLSLKEEDYVLAAKSLGLSHWRILFFHIFPNTFSYTITALTI
                     SVPGYIIGEAALSLLGLGIQEPYPSWGNILSSTIGNLKVVGDAPWLLLAGAFIFAVLI
                     NFNLLGDALRNVLDPKNW"
     gene            12743..13660
                     /locus_tag="PLW07_00230"
     CDS             12743..13660
                     /locus_tag="PLW07_00230"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOL48983.1"
                     /translation="MEKILEIRNLAVVRKQKVNSVPVLNDINFVIEKDELFCLVGESG
                     CGKTTLALSIMKLLPKSFAVKSGEIYVNGKNVLAMDEKSIRNIRGKEMSIIFQDPGAY
                     LDPLFTIGLHMKEALHEGIKTQREMMIYALNEVGLEPYVLHNYPHQLSGGQQQRVMIA
                     MALINKPPLIIADEPTTALDILTTYQIINLLNTIREKYHPGMLFITHDLKLATRIGTR
                     TGIMYNGYIVEIFIPGKTKPVHPYSSILMGAVHKHFTISKTMLSRQSDICPFFEKCPE
                     KEIECQKQIPFVWNKDMTGIRCIKHGRNS"
     gene            13644..14552
                     /locus_tag="PLW07_00235"
     CDS             13644..14552
                     /locus_tag="PLW07_00235"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOL48984.1"
                     /translation="MEEILRCEKISKDFTITDSLGIKKRKLTAVNNISLNIHKHVNLG
                     IVGESGSGKTTLAKILMLLVRPDSGSIYYKGNEITKLSDRKLRFFRNHVRIIFQNPYK
                     SLNPRSTIERVIKEAMLTETRAPAAVENILSHVGLPAEYKNKYPHQMSGGERQRIAIA
                     RALAGNPECIIADEPTGSLDATTEIYILTLLEELKRKFGLTYVFISHNLNIITEFCDR
                     IVVMYRGHVVEQGSVDQISKSPLHPYTKLLWNPESVKSIEKEQKYDYGCPFLHRCIFR
                     TEKCFHQIPELKDGGNEHLVSCLLYE"
     gene            14591..15244
                     /locus_tag="PLW07_00240"
     CDS             14591..15244
                     /locus_tag="PLW07_00240"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOL48985.1"
                     /translation="MATGVPETKKIQRQLTLPLSHAFNMSVRSIKIRFGRSIIVTMSI
                     VLGIAFLMSILSSNAFVTGILQKGPENIKIALQQNLEEMRIRQMWLVSLSLLVCVVGI
                     VNAMLMAVTERSREIGTMKCLGALDKFVVELFLLESSMQGLTGSIIGVIFGGTATFII
                     YLFKYKGLIWSIFPAGLLLKYSAFSVILGIAISVIGAIYPAYVSTKMEPAEAIRREV"
     gene            15258..15998
                     /locus_tag="PLW07_00245"
     CDS             15258..15998
                     /locus_tag="PLW07_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020266656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOL48986.1"
                     /translation="MPKEAIVRTIGLKKTFLLGKIPLHVLKGIDIEIYRGEYISIMGP
                     SGSGKTTLFNMIGGLDKPTEGKVYIDEVDIAQLDAYELAWLRCRKIGYIFQTFNLIPV
                     MTALENVMLPMIFGGMENDEAMNKAKELLETVGLGARLHHKPFELSGGQQQRVAIARA
                     LANDPAIILADEPTGNLDLKTGKEIIHLLKEMNKNKEVSIITATHDLKMLDVSDRVIW
                     IRDGMIERIEDRENLQIKVGEVEGEEAG"
     gene            15998..16807
                     /gene="proC"
                     /locus_tag="PLW07_00250"
     CDS             15998..16807
                     /gene="proC"
                     /locus_tag="PLW07_00250"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOL48987.1"
                     /translation="MHEDKFIFGIIGCGNIGYAITKGLTKYANIPAAFICANDIDQSK
                     AETFKKQLGIQILSLSDLVKKSRFIILAVKPKDISALCRNISSFVENSSIVISVAAGI
                     KIESLNKNFGKKIPTIRMMPNLAVEYGKGLIGYCSENVPESDVKDIVGIFSKIAFCFP
                     VRENEMFLVTAVAGSGPGFLFYVAELIYKFLIKKNFSEEISRKVVAYLFEGTGLMLSN
                     SEAKPSVLKERVCSPAGTTIAGISKLTENNLDLILEMAFEAAEKRAIELSK"
     gene            16831..17667
                     /locus_tag="PLW07_00255"
     CDS             16831..17667
                     /locus_tag="PLW07_00255"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOL48988.1"
                     /translation="MNELSRIKETVAFLKPLTRTKPEIGIILGTGLGKFTERINIEKV
                     IPYNSIPNFPVSTVEGHAGNLIFGTIGNKKIVAFQGRFHLYEGYTASEIALPIRVLKF
                     LGVEVLLESNAAGGLNPMFKAGNIVVITDHINLTGHNPLIGTNYEEIGVRFPDMSNVY
                     DAKIIELAENIALEKKIPIKKGVLVGLTGPNLETKAEYRFLRAIGADMVCMSTVIEVI
                     AAVHAGLKVFGVSVISDMCLPDALKVTTFEEILATAAAAEPFLTDLFESIIIKIQTTD
                     NK"
     gene            17664..17831
                     /locus_tag="PLW07_00260"
     CDS             17664..17831
                     /locus_tag="PLW07_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL48989.1"
                     /translation="MSEKEKTRSDGKKVTQTLVEKTNIRCRVCFIVDGYYPDEKKCRH
                     CGARLFEIDRY"
BASE COUNT         5731 a         3316 c         3495 g         5296 t
ORIGIN      
        1 tttttctttc acttcttttt caagactctc cagaatttct tttccctttt ttccagcaag
       61 agaaagggaa gcaatcccga gagcaagaac cttgtacatc gcattctttt tctcatccat
      121 tttttcctcc gttttaaaaa ataaaattca accagctgat ttcttaagca atcttaccct
      181 tcctttgcca ggacatatgc aaccaatcaa aaaagatctg aaaccgtgtt tctgaacaat
      241 tgatttcacc ctgtcaacat ttcgttctgg cagtgccaga attaatccta tccccatatt
      301 aaaaacattg aacatttctt tttgcattac ttttccagca ctctgtatca tatgaaatat
      361 ctgaggtatc tgccagctat tgagataaac ttcggcgtcc agatgttcag gtaaaattct
      421 cactaaatta ccaggaatac cgcccccggt aatatgagca gcagcatgaa tcaatctgtt
      481 ttcagcaaga gccgaaagta ctgttgaata aatatatgta ggtctgagta attcttctcc
      541 catagacctt cccagagata tctgatatgc tgcaagcatc cttttcatac ctctgcctga
      601 cagtaagacc tttcttacga gcgaaaaacc atttgaatga agcccatttg aagtaatacc
      661 caacaacaca tcatcatttt tcaccctcaa tggtccgagt attttgtctg cctccaccat
      721 gccggcacaa aaacctgcaa ggtcaaaatc atcttcagaa tatacatccg gcatctgcgc
      781 tgtctcacca cctgccagca cacactgcgc tatttcacaa cctttgatta ttccttcaat
      841 aattttttgt tctctttcta tcttcagttt accaaccgca atgtaatcaa gaaacaacaa
      901 gggctttgct ccacaaacag ccacatcatt gcaattcatt gccaccaggt caataccaat
      961 agtatcatat ttttcaaaag cattagcaac aagaattttt gttcccacac catcgcacga
     1021 agaaacaaga aaactttttt tgtttccggt tgaaacaggt ataaatgctc caaaaaaacc
     1081 aatgtcacca gacatttttc tggaaaatga tgcctgtttt acacgttcct taatcaaaga
     1141 gacaaacatt tctgcttttt gatcgttcac accggacttt ttataagtta tcatgatctt
     1201 cccttgtaaa tgtatttata gtaattatat catttaacac aataaaacca ataaaaaacg
     1261 ggcggatgtg attccgcccg tggcaaacca aattttataa acttatttat tcccgtcgac
     1321 ttcccgataa tcagcgtcca cgactttctt atctttttgt tcggattgcg gttcagatgt
     1381 ttgtgcagtc gtctgctgat ctgtctgtcc ggattgagac gtctgtttgt acaagatttc
     1441 tgctatttga tgagagctct tttccacttc ctgcatcagt gtccttattt cattttcatt
     1501 ttttgtttct atcgctttcc tcagggcagc aattttttct ctggcagtat ttctttctgc
     1561 ctcagggatt ttactttcat gttctttcag ggtcttctcc acactataag caagtccatc
     1621 agcctgattg caaagttcaa ttaattcctt ctgttttcta tcctgttctg caaattgctc
     1681 tgcctctctg accattttgt ctatttcctc ctttgtcaat ttttttgagg cagtaatgcg
     1741 aattgattgt tcttttttcg ttgcaagatc ttttgcagtt acggataaaa tcccgtttga
     1801 gtcaatgtca aatgtgacct caatttgagg tacccccctc ggcgcagaag ggattccatc
     1861 cagatgaaat cttccaagac tggtattatg cgcagccatc ggacgttcgc cctgtaaaac
     1921 atgaacttcc acagatgtct ggttatctgc agcagtcgta aatatctgac tttttttcgt
     1981 aggaatagtt gtgtttcttt cgataattct ggtaaacact ccaccaagtg tttcaactcc
     2041 aagagacaga ggggtgacat ccagcaacaa aatatcttta tccttaaggt ctccagcaag
     2101 aattgctccc tgaatcgctg ctcctgtggc aacacattcc ataggatcaa cccctctttc
     2161 aggttttttc cccataaaat cttcaatgaa tttctgtata attggcattc ttgtgggacc
     2221 accaacaaga ataattttgt caatctgtga tggtgtaagt tttgcatctg caagggcctg
     2281 ttccatagga gcgcgacatt tttccactct tgctcttaca agttcttcga gttgcgccct
     2341 tcttattttc atagaaagat gctgcggtcc tttgtcatta gcagtaataa aaggaagatt
     2401 tatttctgtt tcaagagtag aagagagttc aatctttgcc ttctcggctg cttctcttat
     2461 tctctgcatc gccatcctgt cattgatcag atcaataccg gtttgcattt gaaaatcttt
     2521 cactataaaa tcaacaatat cctgatccat gtctgctcca ccaagctgcg tatccccgct
     2581 ggttgaaagt accttaaaaa cgccgcctcc catcatttcc ataatcgtaa catccagagt
     2641 ccctgcacca aggtcaaaca ccatgatttt caattcttga tttaatttat caagaccata
     2701 ggcaaatgct gctgctgtag gttcattaat taatctaaca acttcaagcc ctgcaattgt
     2761 tccagcgtcc ttggtggcct gtctttgatt atcattaaaa tatgcaggta ctgttatcac
     2821 tgctttgtta atcggataac caagaaaatt ttctgtatcc ttttttattt tctgaagaat
     2881 aaacgcagaa atttgctgtg gagtatatgt tttgccatta actgtatatt tgaaatccgt
     2941 acccatcttc ctttttgcac caaaaattgt accttctgga tttactgctg cctgtcttct
     3001 tgctggttca cccacaagaa tctggccatc ctttgtaaat gcgacatagg agggaaacgc
     3061 ctttccacca acaatggttg taccttctgc gcttggtatc acaacaattt taccaccctc
     3121 cattatagca gccgctgaat tacttgttcc aaggtctatg ccaattattt tttccattgt
     3181 ttgtctcctt tttccaattt ttattcacat ttcacacatt tagatgaatt tatataccac
     3241 atagtttcat ttttctactg cttttacaga atgtaagcgc atggtctatt tagcagatga
     3301 aagaattcag aagtaggcgg tgagggaatc gaacccacga cctcttcctt gtaagggaag
     3361 cgctcttgcc gactgagcta accgccttga tttcgcatat tattttacac cgataataaa
     3421 acttgacaaa tgtttcaatt tgctgtataa agtttcgtaa tataaatttt ttctttaaca
     3481 gattaagggg gggagaaatg ttagaggata ttaagcaggc aataatcaga ggagatgcga
     3541 agaaagccac agaacttgtt tcaaaggctc tggctgaaaa tattgcggta gaaaaaattt
     3601 tgaacgaagg tctgattggt ggaatgaatg ttgtcggaat gaaattcaaa aacaacgaat
     3661 tttatgttcc tgaagtattg attgctgcga gagcgatgaa ccaggcaatg gcgattctgc
     3721 agccagaaat tgcaaaagcg ggtatcaaat ctcaggggaa agtggccata ggaacagtca
     3781 agggtgacct ccatgatatt ggcaaaaatc ttgtttccat gatgtggaaa ggagcaggat
     3841 ttgaagtcat ggacctcggc atcgatgttc ccgaagaaaa atttgttgag gctgcaaaaa
     3901 atggtgcaca gttgatcggt ctatcggccc ttctgacaac cacaatgcca gcaatgaaag
     3961 atgttattga aaaattgaaa tcagaaggaa tcagagataa ggtaaaaatc attataggtg
     4021 gtgcccctgt aacacaggct tatgcagatg aaataggcgc cgacggttac tcgcctgatg
     4081 caggttctgc tgttgacaaa gcaaaagaac tgctcgggct tcagtaatag gcttatgaac
     4141 cgtgtctttt ttctcaacga tatactggat tatactgggt tatctttcag gtagtctggt
     4201 atggggctac tggataggaa agtttttcta tggtattgac ctgagaaata aaggcagtgg
     4261 taatataggt gcaacaaatg tattccgggt gctgggacca gtacccggaa tttttacttt
     4321 tgtacttgat gtgttgaaag gggctttgcc ggtaatactg gtgaaaaaca tctatgcaaa
     4381 agatgcaaat cagataatac ttgctactgt gggaatcgct gcaattattg gcagcaaatt
     4441 ttctttgttc ctcagaggca agggaggaaa atctgtaaat tgcagttttg gtgtaatact
     4501 tgcgatgatg cctgttgaag caatattcag tctggttgta tggctcggaa tatttactct
     4561 aacaggatac gtttcactgg catctatagc tgcagcaaca gcattacctt ttctcatatt
     4621 gattttgcaa aaaaatatgc cacttatttt tattggaatt ctcattttca ttgttgttat
     4681 ttttgcccac agagataata taaaaaggtt aattgaagga aaagaaaata ggttcagttt
     4741 atggaaaaaa taggcattgt cggtgcgggt ggttggggca ctgctctttc aataatactt
     4801 tctgacaaat tcgaaataac actctgggaa aagtttccag catacgctac tgtcctctca
     4861 gatacaagag aaaatattga atttcttcca ggatttaaaa ttcctgaaca cgtggctatt
     4921 acaagtgata tacaaactgt ttgtaacaat agttttcttg tgattgttgt accttctcaa
     4981 tatttcagag aaaccatcaa aaatgtgaaa ccgttttata aaagtcagaa ggttttgatc
     5041 gccaccaaag ggattgaacc agaaacagga atgaaaatgt cagaaatatt gtataatgaa
     5101 ataggccagt gtaaatttgc agtcatttca ggaccaacaa ttgcgtcaga agtcgccgca
     5161 gggaaaccaa ctgccgcagt ggtagcttct ggcgacacag aaatggcaag agaaatacag
     5221 tgtatgttct catcaagaat attgagatta tacacaagcg atgatgtatg tggtgttgaa
     5281 atatgtggtg gttataaaaa tgtcatcgca atcggctccg ggattattga tgcacttgag
     5341 cttggagaca atgcaaaagc atcttatctg acacggagca tagaggaaat gaaaagattt
     5401 ggaaaaatgc ttggtgcaaa agaaaaaaca tttttaggcc tcgccggaat cggagacctg
     5461 atcacaacat cattcagccc cagtagcaga aatcaccgat tcggacaggc aatagcaact
     5521 ggcaatgcac aacaatttct tgctcacacc agaatggtta tcgagggtat atctgcttca
     5581 aaagccatca taaaaatggc tgaaacacat aatgtagata tccctattgc ttcatccata
     5641 tacagaattg tgcataagaa cgcaaaacca tctgatgaga ttgaaaaact tatgacaaga
     5701 gaattaaaac atgaatgata gcaaacgtta ttacagtatc aatgaggttt ctgaaattac
     5761 aggactcggc acgcatattt tgagatactg ggaaaaacaa ttttcttttc taaaaattca
     5821 gagagaccct tctggaagaa gaatatattc tgataaagat gttgaaaaga taaaagaaat
     5881 tcaaaaactg ttgtatcaaa atggatacaa aattgtgggg gtgaaaaaga aatttcgtag
     5941 catctcatat acacagaaac aaagcagaca gaattcgtcg ttgttattga aaaaaattct
     6001 tcgatacatc aaggagattg aaaaatgttt accataggtg ttctcgcctc agggaaaggt
     6061 agcaatctta aatctattat agatcagatt aaaataggtt ttttgaatgt tagaataggt
     6121 gtggtcatca gcgacaatcc acaggcatat gccctggaga ttacaagaac tgaaaaaatc
     6181 cccgggatat acatagaacc aggtaaacat aaaacatttc ttgatccggc tcaagaaaaa
     6241 aaatatattc agtgcctgaa ggaacatcat gttgaactgg tttgccttgc aggttttatg
     6301 agagtcataa aaaaagagtt tttcaatgca tatcgaggga aaattatcaa tatccatccg
     6361 tctctactac cttcctttcg cgggcttgag gcatgcaaac aggcacttga atatggagta
     6421 aaatttactg gctgtactgt ccattttgtt gaagaagaca ttgataccgg tccaattata
     6481 cttcaggcaa ttgttcctgt actcggtgat gatagtccgg aaagtttaca taaaagaatc
     6541 caggagaaag aacatataat ttatccagtt gccataaaac tcatttctga aaataaactt
     6601 gttgttcaag gaaggagagt gatgatacat gaagattaac attagacccg ctgtaattga
     6661 cgatgcatat tcgataaaaa atctaataaa tatgcatgcg aaaaaaggaa aaatgcttcc
     6721 tgtttccata aaccagatat ttgaaaattt aagaaatttc tgggtgctct gtgatagcaa
     6781 aggaaaaatt ctgggttgtg catgtttaaa aattgtatgg aaagaccttg gtgaaatcag
     6841 gtccctcgca attaagaatt caaaacaaaa aaaaggatat ggttctgtgt tgattgaaac
     6901 actgatgaaa gaagcagaaa aaattggtat aagaaaaatt tttgtgctca catatgtgcc
     6961 tgaattcttt gaaaagtttg gttttgttcg aatagaaaaa acaaaacttc cacacaaaat
     7021 atggattgat tgtattaact gtccgaaatt cccgagatgc gatgaaatac caatgctgaa
     7081 aaaactatga aaaaatttct attggttttg ttttgtttgt acgggtttca gattcatgca
     7141 atggcggata cacccaatac ccagggactt gaagattttt tgtccggcag ggatttaaaa
     7201 atgcaacgca gttttatgaa taatatgatt gctactggta aaaatctgga attctccggt
     7261 atatcatatg aagatatctt tctttttgga attactatta acttcagtgg cacgtcaggc
     7321 aaagatgtat atataacagg agacaccgtc aaaatttctg gaaaaattag aggaaatcta
     7381 aaggtaatgg caagaaacct aatagtggaa aattgtgaaa ttgatggaaa cgtcaacatt
     7441 ctttctcctg aaatactgat ggctgacagc gtaaagtgca aaaatactac taaaatatgg
     7501 tcaagaaacg ttcagatggg aggacaatat aaaacagtca atataaaaac cagggatata
     7561 atttttacaa aaaatattga tgtgaaaaac ctttttgtac agagtcagac aaaaccttct
     7621 cttccagtca atatgcttaa acattgcaac tttcaatatg aaccaccggt tcctcaaaag
     7681 gcgcaggtat ttttttcgtc taaattcaga aaattatact catttttaag tttgtgtttt
     7741 ccatttcttc ttatgacaat catgacgcca aaagttctgc aggaaacaat agaaatgata
     7801 gaaaaaaaac ctgtctggat ttttttcagc ggaatactac tgctgctgat tgctccattg
     7861 atcctgatac tgttgatggt cacagtgatt ggtgctccaa ttggattgat attgttaact
     7921 ttttatatct ctctgctata tttgtgtcgt ggtttcacct gcgttgcgct gggaaggata
     7981 ctacttttca aattaaaaga gagtaaaatg aaattgattc tcgccataat tcttggtacc
     8041 ggggtttttg tccttttaac cgccatacca aaagccagcg ttattgttca aataccattt
     8101 gtgattttcg gattcggggg ctttgcaata ggcagaattc gcatgttcct gacattaaga
     8161 aaacagaatc ttatataaac aggagaggaa aaatgaagaa aatcgttgta gcgtattctg
     8221 gaggtcttga tacctctgtc attattaaat ggttacaaac aaagtataat gcagaaatca
     8281 ttgccttcac agcagatatc gggcagaaag aaaacccgtc attgcttgaa aaaaaagcgc
     8341 tccagacagg tgcaaaaaaa ttctatttca tggatctcaa ggacgaattt ctctctgatt
     8401 atatatttcc ttcattgaaa ggttctgcgc tctatgaagg caaatacccg cttgcaactg
     8461 ccctttctcg tccattgatc gcaaaaaata tggttgatgt agcaaaaaaa gaaaatgctg
     8521 atgctttcgc tcacggatgt acaggtaagg gcaacgacca ggtcagattt gaacttacat
     8581 tcaaaaatct tgcaccggaa aaggaaatta tcgcacctgt gagagagtgg gaatttaaat
     8641 caagagaaga agaaattgaa tatgcaaaaa aacataatat tcccgtcagc gtgacaaaga
     8701 aaaaaccgta cagtattgac tggaacctgt ggggaatttc catcgaatgc ggtatccttg
     8761 aagacccgtg gaaccaaccc cctgaagacg catatcagat tacacaaaat cccgaaaaag
     8821 ccccatcaaa acccgaatac attgaaatag aattttacaa aggaattcca acaggaatca
     8881 atggacgtca tgtcagtggt ttaaaactta tctctatcct taatgaaatt ggcggtaaac
     8941 atggcgtggg cagaattgat atggttgaaa atcgcctcgt tggtatcaag tcaagagaaa
     9001 tctacgaagc ccctgctgcg gtaatactgc atgctgcaca ttcagaactt gaaaaactta
     9061 ttcttgaaag ggaattatta cattttaaaa caatagtaag tcaaaaatat tccgaactca
     9121 tctattacgg atactggttc agtgatttca gaatctgtct tgaaagattc attgatgaaa
     9181 cccagaaata tgtaacaggt actatcagag taaaattgtt caaaggacaa tgtacggttg
     9241 tcggcagaaa atcttctttc tctttatatg aagaaaaact ctctacatat actgaaaaag
     9301 atatttttga ccatcgagca tcaaagggct tcatcgatat ttatggactc ccgttaattc
     9361 ttgaaggtag aagaaacagg aaaaaagcaa ggtgatatgg aagaagaaaa aatcataaaa
     9421 tggttaaaat ctcattgttt accagaaagg acagaagtaa aaacaggcat tggagatgac
     9481 acagctgtta ttgactttaa tagcaccagt tatcttttgt taactacaga tgctgtcgta
     9541 gaaaacgttc atttcaaaat taatgatgca agtttttatc agataggatg gaaggcactg
     9601 ggagtcaata tcagtgatat tgcggcaatg ggaggcagcc ctctatgggc gcttgtttcc
     9661 attggcatgc ccaaaaaaat tgtttcagca tataaaaaac tgtataaagg gattcttgat
     9721 atgtcaaaat tgtttcatgt ggatattatt ggtggtaatt tatcacgttc agaaatattg
     9781 tttgtggaca tctttcttgc aggaaccgta aaaaagaaaa atcttgtcct gcgcagcact
     9841 gcaaaaccag gagatattct ttttgttaca ggatctctgg gtggatcaca aaaaagaaaa
     9901 cagttcacct tcattccaag agtcagggaa gcacaagcaa tcattagtcg tgtgaaacca
     9961 tctgcaatga ttgacctttc tgatggactg gctgcggatg cgagaaaact tgcagaagca
    10021 tccggttgtg gttttgaaat agccatttca aaaataccag tatcgcagga tgcctgtgaa
    10081 aaggataaaa ttgtttctgc tctgtacgat ggtgaggact atgaattgtt actggcaatc
    10141 ccgcctgaat ctgtcagatc tttacctacc attgttaacg gtatccctgt gacagaaata
    10201 ggtaggctaa caaaaaagaa aacctttgta ctgaaacata caaatggaag cacaaacaaa
    10261 atatcaaaag ataaattcag acactttgaa tgataaaaaa aagattctga tatttgaaac
    10321 agactctgcg gaaaaaacgg aaaaattagg ttctaaaatc gcaggcctgt tgaaaaaacg
    10381 taagattcat tgtgtggttt ttacaggtcc atttggttca ggaaagacga cgatgatcag
    10441 aggaattata aaaaagctca caggtaaaag ccatgttacc agcccatctt tcaccatggt
    10501 tcatgaatac agagaagata acatttcagt atttcattgt gatttttaca ggataaaaaa
    10561 ccctgatgaa cttgaaagta ttggattcag agagtatgaa aacggattcc ttcttattga
    10621 atggccagaa tctgcttttg aaaaaattcc cgaaaattca ttaaaaataa cgctggattt
    10681 tgtcggattg aaacgcagaa aaatcatcgt tgcattacca tgaaaaaact tatactgaaa
    10741 agattgttac aactgattcc gatgctaata ggaatgagtt ttatttcctt catcatcata
    10801 caatcttcgc ctggagatta tttcgcacag atgaaaatga atccagaaat ttctccagaa
    10861 acaatagaga tgatgagaac aagttttgga cttgacaggc ctctgtttgt tcagtacttt
    10921 ttctggttga aaaatatatg taaaatggat tttggaattt cctttgcgta tcacattcct
    10981 gtaacgacat taatcaaatt cagacttaaa aacacattgt gccttgctat tttttctctc
    11041 tttctaagct gggtcatagc aatccctctg gcagtactgg caggatataa agaaggaaca
    11101 ctgattgaca aaattttttc cttgtttgca tatctttcaa tttctcttcc atcttttttt
    11161 gtcgcattac tctttgttta tttcatcagc aagacaggcg ttttacctct tggaggcacg
    11221 agaagcattt ttgatataga cgcaaacgct ttcttaatat ttaaggatta catgagacat
    11281 cttcttgtgc caggactggt actgtgtttt gtgaatattg gctggctttt caggcttatg
    11341 aaaaataatt ttattgaagt atacaacact ccatatatac tggcggcatg ggcaagggga
    11401 ctccctgcaa gttctgttat attcaaacat acattaagaa atgcactgaa tccaatgttc
    11461 acgattttgg ggatggaaat aggaagtatt ctcaatggtg ctgcacttac agaaataata
    11521 tgcggatggc caggaatggg tagtcttatt ctcgaagcag tactttcgca tgatttgtat
    11581 cttgtaatgg gatctcttgt tattagtgga tttcttttaa ttattggcaa ccttatcggc
    11641 gatattttga tagcgctttt cgacccgaga atcagattaa catgaaaata tttttccaga
    11701 gattaaagaa tcatagtaaa cttgcgtatt tcagtttctg gttcctcatt ttcttttaca
    11761 ttgtttcatt tcttgctgat ctactggcgc cttatcactt tgatcatcaa ttcaggaaca
    11821 aaccgtatca tccaccaaca aaaattcatt ttgttgacga aaacggacat tttcacatca
    11881 ggccatttgt ttacaacatg gtcgtggatg atcctgtttt cagaacatat cgtgaagata
    11941 aatccatcag ataccctgtg aaattcttcg tcgatgggcc tgaaagaaaa atctttttta
    12001 ttttcaaaac aacaagatat tttttcgggg ttgacgaacc ggctggcatt ttcctgtttg
    12061 gaactgatac ctatggcaga gatatttttt caagattact gtacggtgga agaatttctt
    12121 tatctatcag tattgtggga gtattgattt cttttacgct tggtatgatt acaggaggta
    12181 ttgcgggtta ttttggtgga ttacttgacc agattctcat gaggtttgta gaaattctta
    12241 tggttttccc ttctttttat cttatgcttt ctctcagggc aatgtttcca cttaaccttt
    12301 caagcatcca ggtatatttt ctcatcgtac ttattctcag tttgattggc tgggcgggac
    12361 tggcaaggat tatcaggggc atggtacttt ccctaaaaga agaggactac gtcctggcgg
    12421 caaaatccct cgggctttct cactggagaa ttttattttt tcacatattt ccgaatactt
    12481 tctcttacac aatcaccgca ttaaccatca gtgtcccagg gtatattatt ggagaagccg
    12541 cgctgagtct tcttggtctt ggcatacagg aaccatatcc cagctgggga aatattctat
    12601 cttcaaccat tggaaatctg aaagttgtcg gagacgcgcc ttggctgtta cttgctggag
    12661 catttatatt tgcagtatta attaatttca acctccttgg agacgccctc agaaatgttc
    12721 ttgatccaaa aaactggtaa acatggaaaa gatccttgaa ataagaaatc tcgcggttgt
    12781 aagaaagcaa aaagtgaatt ccgtaccggt attgaatgat atcaattttg ttattgaaaa
    12841 agatgaacta ttctgtcttg tcggggaatc tggctgtggt aaaacaaccc ttgcgctttc
    12901 tatcatgaaa ttgcttccaa aaagtttcgc ggtgaaatcg ggtgaaattt atgttaatgg
    12961 taaaaatgtg ctggcaatgg atgaaaaatc aataagaaat atccggggta aggaaatgag
    13021 cattattttt caggatcctg gcgcgtatct cgatccactt ttcacaatcg ggctgcatat
    13081 gaaagaagca ttgcatgaag gaataaaaac ccagagggaa atgatgatct atgcattgaa
    13141 tgaagttggc cttgagccat atgtattgca caattacccc caccagttaa gtggaggtca
    13201 gcaacagaga gtaatgattg ctatggctct gatcaataaa ccacctctca tcatagcgga
    13261 cgaaccaaca actgcccttg acattttgac aacctatcag attattaatc tcttaaacac
    13321 aatacgcgag aagtatcatc cgggtatgct tttcatcacc catgatctga aacttgccac
    13381 aagaatagga acacgcacag gaattatgta caatggatac attgtggaaa tttttattcc
    13441 aggcaaaaca aagccagttc atccatattc cagtattttg atgggtgcag tacacaaaca
    13501 ttttacaata tctaaaacaa tgttatcgcg acaatctgat atatgtccct tttttgaaaa
    13561 atgtccggaa aaagaaattg aatgtcagaa acaaatccca tttgtgtgga acaaagatat
    13621 gacaggaata aggtgcataa aacatggaag aaattcttag atgtgaaaaa atcagcaagg
    13681 attttacaat cacggattct ctcggcataa aaaagagaaa actcacggcc gtcaacaata
    13741 tctccctcaa catacataag catgtgaacc ttggtatcgt tggagaatcg ggttcaggaa
    13801 aaacaaccct ggcaaaaata ttaatgttac tggtcagacc tgattcagga tccatttatt
    13861 acaagggtaa tgaaataaca aaactgtctg atagaaaact tcgctttttc agaaatcatg
    13921 taagaataat ttttcaaaat ccatataaat ctttgaatcc gcgatcaacg attgaaaggg
    13981 taataaaaga agccatgcta acagaaacaa gagccccggc cgcagttgaa aatattcttt
    14041 cgcatgttgg attgcctgct gaatataaaa ataaatatcc tcaccagatg agcggaggag
    14101 aaagacagag gatagcaatc gcaagggcgc tggcaggtaa tccggaatgt atcattgccg
    14161 acgagccaac tggcagcctt gacgcaacaa cagaaattta catcctgaca ttgcttgaag
    14221 aattgaaaag aaaatttggt ctcacatacg ttttcatttc acataacctg aatattataa
    14281 cagaattctg tgacagaatt gtggtaatgt acagaggtca cgtcgttgaa cagggttcag
    14341 ttgaccagat atcaaaatct ccattacacc catacacaaa attactgtgg aacccagaat
    14401 ctgtaaaaag tattgaaaaa gaacagaaat acgactatgg atgtcctttt ctccacagat
    14461 gtatattcag aacggaaaaa tgtttccatc aaatccctga actgaaagat ggagggaacg
    14521 aacatcttgt gtcctgtctt ctttacgaat aaaataaatt gggttaaaat ctattaaaaa
    14581 aggagatcaa atggcaacag gggtaccgga aaccaaaaag attcaaagac agcttacatt
    14641 gccgctcagt catgcgttta acatgagtgt tcgcagcata aaaataagat tcggtaggtc
    14701 cattatagtc acgatgtcca tcgtgctcgg tatagcgttt ctgatgtcca tattgagcag
    14761 caacgcattt gttacaggaa tactgcaaaa aggaccggaa aacataaaaa ttgctctcca
    14821 acaaaatctt gaagaaatga gaattcgtca gatgtggttg gtttcacttt cgctgcttgt
    14881 ctgcgttgtt ggaattgtaa atgcaatgct tatggcagta actgaaagat caagagaaat
    14941 tggaacaatg aaatgccttg gcgctctcga taaatttgtc gttgaacttt ttcttcttga
    15001 atcctcgatg cagggactga caggttcaat aataggagtg atatttggtg gcactgccac
    15061 atttatcatt tatcttttta aatataaagg actaatctgg agtattttcc ctgcaggact
    15121 attgctaaag tactctgctt tcagtgtaat tctgggaatc gctatatctg tcatcggagc
    15181 catctaccct gcctatgttt cgacaaaaat ggaaccagct gaagcaattc gcagggaggt
    15241 ttaaaaagga gaataacatg ccgaaagaag ccatagtaag aacgatagga ttaaaaaaaa
    15301 catttttact tggaaaaatc ccgcttcatg ttttgaaagg cattgacatt gaaatttata
    15361 gaggagaata catatcaatc atgggacctt ctggaagcgg aaaaacaaca cttttcaaca
    15421 tgataggcgg tcttgataag ccaacagaag gcaaggtgta tattgatgag gttgatatcg
    15481 ctcaacttga tgcgtatgaa ctggcgtggt taagatgcag aaaaatcggt tatatatttc
    15541 agacatttaa cctcataccg gttatgacag cccttgaaaa tgtcatgcta ccaatgattt
    15601 ttggtggcat ggaaaatgat gaagcaatga acaaagcaaa ggaattactt gaaacagtcg
    15661 gtcttggtgc gcgattgcac cataagccat ttgaattgtc aggtggacag cagcaacgtg
    15721 tggcaatcgc aagagcactt gcaaatgacc cggcaattat tcttgctgat gaaccaacag
    15781 gaaaccttga cctgaaaacg ggtaaggaaa tcatccacct ccttaaggaa atgaataaga
    15841 acaaagaagt aagcatcatc acagcaactc acgatcttaa aatgcttgat gtgtcagata
    15901 gagtcatatg gatcagagac ggtatgattg aaagaataga agacagagaa aacctgcaaa
    15961 ttaaagtcgg agaagtggaa ggagaggaag caggttgatg cacgaggata aatttatttt
    16021 tggaatcatt ggttgcggaa acataggtta tgctattacc aaaggattga caaaatatgc
    16081 aaatattcct gctgcgttca tctgcgctaa tgatatagac caatcaaaag cagaaacgtt
    16141 taaaaaacaa ctgggcattc aaattctgtc tctttctgat cttgtgaaaa aatcacggtt
    16201 cataatactt gctgtcaaac caaaagatat ttctgcatta tgccgcaata tttcttcttt
    16261 tgttgaaaat tcttctattg tgataagcgt tgcagccggt attaaaatag aatccctgaa
    16321 caagaatttc gggaaaaaaa tacccaccat cagaatgatg ccaaatcttg cggttgaata
    16381 cggtaaaggc ctcattggtt attgttctga aaatgtgcct gaatctgatg taaaagacat
    16441 tgtggggatt ttttcaaaga ttgcattctg ttttcctgtc agggaaaacg aaatgtttct
    16501 tgtaacagca gtcgctggca gcggtcctgg ttttctcttt tatgttgccg aattgatata
    16561 caaatttctg ataaagaaaa acttttctga agaaatttca agaaaggttg ttgcatatct
    16621 ttttgaaggc acgggactga tgctttcaaa ctctgaagca aaaccttcgg tcttaaaaga
    16681 acgggtgtgt tctccagcag gaacaaccat agcaggtatt tctaaactaa cggaaaataa
    16741 tctcgattta attctggaaa tggcttttga agccgcagaa aaaagagcga ttgaactgtc
    16801 aaaataacac aatcgatatc aggaggaaat atgaatgaac tctccagaat caaagaaact
    16861 gttgcgtttc tgaaacccct tacgcgaacg aaaccagaaa taggtattat ccttggaaca
    16921 ggcctcggta aattcacaga gagaatcaat attgaaaaag ttattcctta caattccatt
    16981 ccaaatttcc ctgtctctac tgttgaaggt cacgcaggta acctcatctt cggaacaatt
    17041 ggaaacaaaa aaatcgtcgc tttccagggg agatttcacc tttacgaagg ttatactgca
    17101 tcggaaattg ctcttccaat cagagttttg aaatttcttg gggtcgaagt tttgcttgaa
    17161 tctaatgccg caggcggatt aaacccaatg tttaaggcag gcaacattgt agtcattact
    17221 gaccatatca atcttaccgg acacaacccc ctgataggaa ccaattacga agaaattggt
    17281 gtaagattcc ctgacatgtc caatgtatat gatgcgaaaa tcattgaact tgccgaaaat
    17341 atcgcccttg agaaaaaaat ccccatcaaa aagggtgttc tggtgggatt aacaggtccg
    17401 aatcttgaaa caaaggcaga atacagattt ctcagagcaa tcggcgctga catggtttgc
    17461 atgtccacgg ttatcgaagt tattgccgca gtgcatgcag gtttaaaagt ttttggtgta
    17521 tctgtgataa gcgatatgtg cctgcctgac gccctcaaag ttacaacctt tgaggaaata
    17581 cttgcaacag ccgcggcagc agaacctttc ttgacagacc tttttgaaag tattataatt
    17641 aaaattcaga caacggataa caaatgagcg aaaaagaaaa gacaagatca gatgggaaaa
    17701 aagtaacaca aacgttggtg gaaaaaacca acattcgctg ccgggtatgt tttatcgtag
    17761 atggatatta tccggatgaa aaaaaatgca ggcattgcgg tgcccgcctg tttgaaatag
    17821 acaggtatta gggagggg
//
LOCUS       DAORYU010000003        17650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_2636_length_17650_cov_4.900426, whole genome shotgun sequence.
ACCESSION   DAORYU010000003 DAORYU010000000
VERSION     DAORYU010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 17650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17650
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2636_length_17650_cov_4.900426"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="PLW07_00265"
     CDS             <1..556
                     /locus_tag="PLW07_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL48990.1"
                     /translation="SGAGSGSSWRMCWVTLGFLDPLVQKGYLQGQFFWCNKPGFEHYA
                     GTCSDGTPNWLLYTTTGSINCPCLDPYQRYNLSAYDRTSYVYSSQVDFGYNNRLPNTA
                     KKLGRVRRPGETIMFFESRYGEYDINNASTAATLFPIFQPPGYYSGSGGRHQQIELVN
                     AVFVDGHAKAVNQDEYVNGLAYTP"
     gene            complement(766..2589)
                     /gene="mnmG"
                     /locus_tag="PLW07_00270"
     CDS             complement(766..2589)
                     /gene="mnmG"
                     /locus_tag="PLW07_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOL48991.1"
                     /translation="MPEREIHIVVIGAGHAGIEASLASSRMGLHTLLITGNFGTIAKA
                     SCNPAIGGVGKGQIVKEIDALGGEMALATDSTGIQFRMLNTGKGRAVWSPRAQIDRIK
                     YSDYMNQVLFNQKNLKLLQAMVVNIIEKNGSIEGVILDSGETIKCRCVILCPGTFLNG
                     IIHIGNQCFPAGRMGEFASKGITECLRNLGFETGRLKTGTPPRISKQSIDFSKVTQQP
                     GDERPVPFSYRTSFIKREQISCYLTKTTSETIKIIRDNLDRAPLYTGQIRSTGPRYCP
                     SIEVKVVKFPDRDTHHIFLEPEGYDNDEVYVNGFATSIPVDVQEKALKKVIGLQNVRI
                     LRYGYAIEYDFVPPYQLKPTLETKNIKGLYLAGQINGTSGYEEAAAQGLIAGINAASS
                     ILEKEPLILKRHQAYIGVLIDDITTREITEPYRMFTSRAEYRLMLRQDNADFRLMDYG
                     FKYGLIDEKTYQTMGKNRVLTKKFCEMLEHTYLSGYQEKTSLADFLRRPQHGISDIFQ
                     FCKDLEHVPEHVKEEIEIEIKYAGYIKRELEMIEKTSHMDSVKIPENIDFSKIKGIST
                     EAIEKLSKYRPFTIGQAKRIDGVSPSDITAILIHLKKSVIK"
     gene            complement(2582..3781)
                     /locus_tag="PLW07_00275"
     CDS             complement(2582..3781)
                     /locus_tag="PLW07_00275"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HOL48992.1"
                     /translation="MILPFKGYRYSKKAGNLEQLIAPPYDVIGQQYRSELIARSPYNI
                     VHLTLPESFDAAYHSVIAQTLNKWCKDGILQQDGKEYFYLMKQTFPCEGKILERTGFI
                     GLFDLRASDRIIRHEVIFNKYKDDRIRLLEATKSNLEPIFLLYEDHQHFIEKFACSTA
                     FEEKLSFEETTVELHTCKQEVLLPLLEKIQNSTLFIADGHHRFQASYQYFQTNPDTPP
                     YIMVYLTNLVSRSLVVLPTHRALKNVNPSEKAHIIEEFFDIKKQENLNQTLSSIEHSQ
                     YCSVGVYFNHSFQTWTVKNPEKLQQVLPQHYSDTLKSLDVVMLHYLVIEKIFGANAKE
                     KLYYARNPEEIVKFVDSNQESIGFFMQKPDLMKIKQVSIGGEILPPKTTFFYPKVPSG
                     LVIARYA"
     gene            complement(3784..4542)
                     /locus_tag="PLW07_00280"
     CDS             complement(3784..4542)
                     /locus_tag="PLW07_00280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOL48993.1"
                     /translation="MEKLNNIVKFLDGFLNINNIKDDSWNGLQWEGTEKVEKIMCTVD
                     AGADTFQEAVKRNANLIIVHHGNYWKNANPSFTKANKKRMEILHRHNISLYAAHLPLD
                     MHPEIGNNILLLKMMGAKKTTGFYPYDGQYISFCGQFTKPKKLDDIVSTINGCLGITC
                     RLLPYGPDKIKTVAVISGGGGYAGFNEAIKAGVDLYVSGDTLEVFHSAKDYGINVIFG
                     GHHATETLGVEELAKVISKNFDVKAEFVDIPTGL"
     gene            complement(4556..7369)
                     /locus_tag="PLW07_00285"
     CDS             complement(4556..7369)
                     /locus_tag="PLW07_00285"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HOL48994.1"
                     /translation="MPVVKLKITMKKILLLFVLAGACFAGISVDWSIPATTDTPFVIP
                     FSNITVDGVLTDTEWASGFCLPIRSQYHISNAKRQWKGPQDAGMEFYAAWNEKGLFFG
                     AIVADNEVINHKPLESAYEQDCIEIFVDGRSISFMKPPYSKGCYQILVKPPIDRQAPI
                     AEVFGRNKISGMQIAGVKTITGYDIELFVPWSAFPEIKKVCPGIHIAIQAMLDDYDSQ
                     DLDKVQPLSLSYQGKKELYRSPQHFVHCVCEEKIQPVFSIECSPLMLEKQSIPVAVET
                     FSMAGDIDSIKIRIENPQGIVLAEKKPKLIKYTSPWPKAIRATASFDVEKLQGDVFVI
                     SAIVGRKNAEYTCRKTVIYLGNIAEEILSEIRKANIKKLSQSDPFKAAGFLAVGSCYE
                     KIKYAVETADVEKLTYAVREIAARINALNGKQLKKTHSLIDLLVLTENPESQVVVEYP
                     LPEVANITFYWGSVPLVTVNVRKFADQNLPKQAAKEKITGFVDLLEDVNPAGPVVIAG
                     LPARASSWSYMFFYFNIEKFDPERQILVAIPSSRTVYAVDTGKIDYVDAEAATIVDGA
                     SETIKKQVEKYIRSKKIHLLPVDEALKKNSVLIATEKDIPQEIKKFKAYQVSIVKQSL
                     IRIPYNDMLVSVSHPSRWVAEEAAKLVIAGKPVSIQDVDKIRKTLVNEFAFSTRSSEN
                     VKRQGFIYCGDLHSHSRFSDGKHTPVGMALQSMTCCMDFLALTDHNTIDGALITSTLL
                     SKHGFDYTFIVGQEITTKNFHFNAYPLKKVIQWTLAPEQIISLAHQQSAIIQWNHPGW
                     THSMWELERIDEVPSDIGLDGWEHIPFHYYDWKKNDTLPSLIGSTDTHDGTFSNPERT
                     IIFADGCQEKDVVEAIKNKRTILVSPSRGSDFMYGENSVIGEAWDILLEAQELKNVKK
                     QHISNMLKNADLPALLKEKYSKN"
     gene            complement(7356..8300)
                     /locus_tag="PLW07_00290"
     CDS             complement(7356..8300)
                     /locus_tag="PLW07_00290"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOL48995.1"
                     /translation="MKYIQLGKTDLIVSRICFGCWQLSPRFWGNIPLDEWNAAMKKAM
                     DCGINFVDTAQAYGDGFAESVLGDFMSKYNCRKELIIATKFYWNIDDPENRYPDTGFQ
                     YIINECEASLKRLKTDWIDLYQIHAWDPLTNPEEVAAALLLLKKQGKIRWIGVSNMNV
                     HQMNMYIRYFDIDCLQPLYNILDRDAEKELFPFCLEKKIGVITYSSLARGILTGKYSG
                     NETFEDSRANHPRFTGENFRKITNAVNTIMKPVSEKLGITVAELAVRWILTHPAVTSA
                     IVGVKKVQHIESVLKAPDSHLDRQLWHKIANEMANACS"
     gene            complement(8313..9581)
                     /locus_tag="PLW07_00295"
     CDS             complement(8313..9581)
                     /locus_tag="PLW07_00295"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOL48996.1"
                     /translation="MELSLKKKLLLFLLLNCFYFFSYFHRVAIPGTIFNELQINFHLT
                     ANQVAGLGTVVFYIYGLLQFFVGPCLDRFGTNRIFVGGGFLLCFGSILFAIAHSIFLL
                     YLSRILVGIGASVAYLGLVKKTDELFGRDFFSIMLGVALFLGYSGGLFATLPFERLVH
                     QFGWRNTILVMGVILFFVMTVILSTIAKQHQFSQQKNSFSLDDIKSVILNKKIHPVLI
                     VAAINFTIYFVFQTIIGKKLIQDICGLSSKIAASFTLIMMGTTMVAALLWGLVVHLIK
                     QKKVLVIAVCLASMISCIFMLTNLILFKIPVVFLLCFFIFALTGGSGPLTTTLIKELG
                     PSSGVGTAVGFVNGACYISVAIAGNIAGLIMDRFSSKAIVTPDAIIYPVPAYVTITTV
                     CLFLNLVSFIVSFKIRESEKEIEFEVPAPL"
     gene            complement(9584..11746)
                     /gene="glgB"
                     /locus_tag="PLW07_00300"
     CDS             complement(9584..11746)
                     /gene="glgB"
                     /locus_tag="PLW07_00300"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HOL48997.1"
                     /translation="MDERLLKLVNAEYHNPHEILSWQNADTGFILRAFVPDAKNVSVT
                     NIDTSEKYNLISVEGGIFEGHFDEKFQYRIEAKFPDGTQYCFLDPYAFNTSIFGEMDL
                     YLFNEGNHFEIFKKMGAVHRTINGISGYNFSVWAPDARRVSVVGDFNRWDGRKHQMRV
                     LGNSGIWEIFIPEFQPGNLYKYEILTRDHSIILKSDPYGAYFECRPNTASITWTSHYH
                     WEDEKFLNQQTDFLNKPLSIYEIHLGSWKRKQDGSFYNYRELAPQIVDYVKSLNFNCI
                     ELLPVAEHPLDASWGYQATGFYAPTSRYGNPDDFRFFVDYCHKNNIRVILDWTPAHFP
                     KDSHGLYRFDGTHLYEHMDQKKGEHPDWKSAIFNYGRYEVSNFLIGSGLNWIENFHID
                     GLRVDAVASMLYLDYSRKEGEWIPNIYGGRENLEAIEFIKKLNTAIYGRNKHSFTVAE
                     ESTAWPGVTKPVYLGGLGFGFKWNLGWMHDTLSFFSKDPIYRKHHFNSLTFSLLYAFS
                     ENYILPLSHDEVVHGKRALVEKMPGDNWQKFANLRLLFGWQFGHPGKKLLFMSGEFGQ
                     WNEWDHAKQLDWELLNDENHKGIFNLVKDLNTLYLQNPQMHENEQSHECFQWIDFSDV
                     DNSVVSFLRWNRKKDKCLVFVFNFTPVPRYQYRIGVPFSGEYKEILNSDSFLYRGSNT
                     GNFGLVQAEKQTMHHFDFSIEIVLPPLGFVVFSPAQRN"
     gene            complement(11739..12878)
                     /locus_tag="PLW07_00305"
     CDS             complement(11739..12878)
                     /locus_tag="PLW07_00305"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOL48998.1"
                     /translation="MIKFSSSFECGNGKHFSQISENRYRCEVTGDKKVYCYYFCFELE
                     NSGTDTSFVIEVWHDPVINDPEGFISHFPTTIWISNDDGKRFHPLEQSCCQTMDNHIE
                     ISLALKQGKKLIITNNWPCTYSNTCVFLKNLVEQRKNCTLFSLGKSAGNREIFGIKSG
                     NPAGPRILCLAGQHPVEFPGIWAVRAIADFVTSGIPQAETLREHFLFEIIPIVNPDGN
                     VAGRNCFTDEGVDLYQVFSDNPDMDKPDSTEGKLLWRWATEQRTDLWINFHCYLGWRT
                     NSEYPYDGWYEVPYELFKDSTKRKIYQALCDTMRLLTDAPSTSTQPDTHWINSFEHQF
                     AKRFSIPHVFYEINGGTAGPFQSAKRGLTVFRNAMTTFQHVIKNG"
     gene            complement(12888..14861)
                     /locus_tag="PLW07_00310"
     CDS             complement(12888..14861)
                     /locus_tag="PLW07_00310"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HOL48999.1"
                     /translation="MTYLNILWHFHQPLYLPPDSNRMDNATIAFRVLYNYYPMALMIK
                     NSGMPLTCNFTPSLILQINKIVEHQIIDGFQALLQDENANDTEKISVFLDDLPARTKT
                     RHNIFRKLIDKFYSKNISGSEIQDLKMWMHLACIHPFLLGYFPEIAQLYEKAVGFNNK
                     DRQILIDFEQKVFSEILALYRNLYQSGQIEISTTPGYHPIMPLVYDLSTARNTSTHLE
                     IPSIEFSYPEDVKAHIAMGIETANSVFGKTINGIWPAEGSISNEVLDILMEYGIQWLG
                     ADEQILQYSGCQEKDISSTLYRWKNTFNIFFRNHQFSDRIGFIYHSWEESAAAEDLVK
                     KIKEFSEGREKILTIILDGENPWEWYRQEGSVFLTRLYERVLSDGNIKPVTFSQACKL
                     NFKTVSLDSIPAGSWMGLNFDNWVGKPDANKLWQILADARKTAEQHASKQGIQEQLNN
                     LSLMAESSDFFWWMSVPAEQSTKIKFYSLFQSIISKIYRTAGLSVPFEVTKAFVPVTN
                     IKQPENYITAVIDGRITNFFEWYGASEIDIIRIWTTFQPFDLPVKKLFYGYDRNNLYI
                     RIDISQKVFSSISIETEDRGIIFSFDITKETFIDENIALDECIEIKIPWEKIGTDQVH
                     LLIRMKTAESEISIPPAGYIVFEKQSFDDEWAA"
     gene            complement(14858..16282)
                     /gene="glgA"
                     /locus_tag="PLW07_00315"
     CDS             complement(14858..16282)
                     /gene="glgA"
                     /locus_tag="PLW07_00315"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HOL49000.1"
                     /translation="MKSVAIVCCECVPFVKVGGLADVTGALLVKLPKYVDTKVFLPAF
                     SQITRKYPCDVVGEYEVSFSQDRIEKAILLRMKDKHNVYLIANNHYFDRDQLYGESGI
                     DYPDNLLRFSFFAKAVLECCKQSGISVDVFHCNDWHTALLPLYKKLFYPDTGTASVFT
                     IHNLAYQGIFPASQFPALGLGGEFFSMEQLEFYGNINLMKAGIIHSDVINTVSPTYAK
                     EILSAEFGCKLEGLLKTREKDLVGILNGIDYLVWNPGFDSAIFKKYRTRKGKLVNKTM
                     LQDKFSLPASVETPVFGFVGRFVQQKGIDLIVAAVESLKEKDFQFIGLGTGERVYEEA
                     ITLLAKKYPDRVAVKIGFDDALARQIYAGSDFFLMPSRFEPCGLGQLISLKYGTIPIV
                     RKTGGLADTIQPINVETQEGWGFVFEDADFSQLVQAMNMAIDLYPDTTFMEHLFKKAI
                     SLDFSWNKSVEQYIALYQRAFEKR"
     gene            complement(16306..17334)
                     /gene="galT"
                     /locus_tag="PLW07_00320"
     CDS             complement(16306..17334)
                     /gene="galT"
                     /locus_tag="PLW07_00320"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HOL49001.1"
                     /translation="MPELRKDPVIGRWVIIATERKLRPSDYAEMCSTVGEEKAPFCPF
                     CPGNETKTPPEIFAIRDNNTQPNTPGWLVRVVPNKYPALKIEGNLDKKGVGIFDKMNG
                     IGAHEVIIETPEHNQQMHDFDTQHLEKVIHTYQIRSLDLKNDTRLKYSMIFKNYGKEA
                     GASLHHSHTQLIATPVTPKRVKEELKGTQWYYDYKERCIFCDIIEEETSRGERVVAMN
                     KDFIALVPYASRFPFELWILPLKHRSDFDSISDVERITLAEILGSILKKLMKGLCNPA
                     YNFIFHTAPNRFPHPGYWQTIDKDYHWHIEIMPRLTRPGGFEWGTGFYINPTPPEEAA
                     QFLRDLVV"
     gene            complement(17354..>17650)
                     /gene="glgC"
                     /locus_tag="PLW07_00325"
     CDS             complement(17354..>17650)
                     /gene="glgC"
                     /locus_tag="PLW07_00325"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450359.1"
                     /note="catalyzes the formation of ADP-glucose and
                     diphosphate from ATP and alpha-D-glucose 1-phosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOL49002.1"
                     /translation="TGMVLDSIIGGGCIISGGRVYRSVLSPWVRINSYADVSESILLD
                     RVNVGRYCKIKRTIIDKNVVVPQGTIIGYDPVEDRKRFVVSETGIVVIPKDYQW"
BASE COUNT         5014 a         3585 c         3325 g         5726 t
ORIGIN      
        1 ttcaggagca ggatcaggaa gttcatggag gatgtgttgg gttacacttg gtttccttga
       61 tccccttgtt cagaaggggt atcttcaggg acaatttttc tggtgcaata aacctggttt
      121 tgagcactat gctggaacat gctcggatgg tacaccaaac tggttgcttt acacaaccac
      181 tggtagtatc aattgcccct gtcttgaccc atatcagagg tataatcttt cagcgtatga
      241 caggacaagt tatgtgtatt ccagtcaggt tgactttggc tataacaatc gtcttccgaa
      301 tacagcaaaa aaactgggaa gggtaagaag accaggtgaa acaattatgt ttttcgagtc
      361 gcgctatgga gagtacgaca ttaataatgc atctacggcg gcaacattgt ttcctatatt
      421 tcaaccaccg ggttattaca gtggctctgg aggaaggcat cagcagatag aactggtgaa
      481 tgctgtattt gttgatggac atgcaaaggc agtcaaccag gatgagtatg tcaacgggct
      541 tgcgtacacc ccgtaaaatg ttttacagat aaaagccatt acatattttt cttccaagtt
      601 tattctggct taccatctgt agagaagatg gttggggtgt gtattttaag gtagtgaggg
      661 atttcatgaa tatcaagccc atatggtgtt aatctttcca gacaatgtca tccatgcctt
      721 gttcaggttc gcttatcccg ccatcatcct aatgccatat cacggttatt ttatcacaga
      781 ttttttgaga tgtatcagta ttgctgtgat gtctgatggg cttacgccat ctattctttt
      841 tgcctgtcct atggtaaacg gtcgatattt tgatagtttt tctattgctt cagttgatat
      901 acctttgatt tttgaaaaat caatgttctc aggaattttg acgctgtcca tatgggatgt
      961 tttttctatc atttcaagtt ctcttttgat atatccagcg tacttgattt ctatttcaat
     1021 ctcttccttt acatgctctg gcacatgctc aaggtcttta cagaactgga aaatgtctga
     1081 aataccatgc tggggtcttc gcaaaaaatc tgcaagggat gttttttcct gataacctga
     1141 cagatatgtg tgttcaagca tttcgcaaaa tttttttgtc agaactctgt tttttcccat
     1201 tgtttgatag gttttctcgt caataagccc gtacttaaaa ccatagtcca tcaatctgaa
     1261 atcagcattg tcctgtctta acatcaatcg gtattctgcc ctggaagtaa acattctgta
     1321 tggttctgtg atttcccttg ttgtgatatc gtcaatcagg acaccgatgt atgcctggtg
     1381 tctttttaat atcaggggtt ctttttcaag tattgatgaa gctgcattga taccagcaat
     1441 aagtccctgt gcggctgctt cctcataacc gcttgttcca ttgatttgac ctgcaagata
     1501 cagccccttg atattttttg tttcaagtgt gggttttaat tgatagggag gaacaaaatc
     1561 atattcaatc gcatagccgt atcgcaagat tcttacattt tgcagcccga taactttttt
     1621 cagtgctttt tcctgaacat caacaggaat actggttgca aatccattga catacacttc
     1681 gtcattatcg tatccttctg gctcaagaaa aatatgatgg gtgtctctat caggaaattt
     1741 tactaccttt acttcaattg atgggcaata tcttggacct gtggagcgaa tttgacctgt
     1801 gtacaaaggc gccctatcaa gattatccct gataattttt attgtttctg atgttgtttt
     1861 tgtaagatag caggaaattt gttctctctt gatgaacgat gttctgtaag agaaaggaac
     1921 agggcgttcg tcgcctggct gttgtgttac tttgctgaaa tcaatggatt gtttgctgat
     1981 tcttggaggt gttcctgttt ttaatctgcc tgtttcaaac ccaaggtttc tcaaacattc
     2041 tgtaataccc tttgaggcaa attcacccat tcttcctgct gggaaacact gattgccgat
     2101 gtgaataatg ccatttaaaa aagtaccagg acagagaatg acacacctgc atttaatggt
     2161 ttctcctgaa tcaagtatca ctccttcaat gctgccattt ttttcaatta tgttgacaac
     2221 cattgcctgc aacaacttca aatttttttg attgaaaagc acctggttca tataatcaga
     2281 gtatttgatt ctatcaatct gtgccctcgg actccacact gcgcggccct ttccagtatt
     2341 caacattctg aattgaatac ctgtgctgtc tgttgcaaga gccatctcgc caccaagggc
     2401 atcaatttct tttacaatct gaccttttcc caccccacca attgcagggt tacagcttgc
     2461 ttttgcaatg gttccaaaat tacctgtgat gagaagggta tgtagcccca tccttgatga
     2521 ggcaagagat gcctcaatgc cagcgtgtcc tgcgccaatg acaacgatgt gaatttctct
     2581 ttcaggcata tcgcgcaatt acaagtcctg atggcacttt tggatagaaa aatgtggttt
     2641 tagggggtag tatctctcca ccaatagaga cctgtttgat tttcatcagg tctggtttct
     2701 gcatgaaaaa accaatactt tcctgattgc tgtcaacaaa ttttacaatt tcttcagggt
     2761 ttctggcata atacagtttt tcttttgcat ttgcaccaaa aattttttca atcacaagat
     2821 aatgaagcat tacaacatca agagatttca atgtatcaga ataatgttgt ggtaaaacct
     2881 gttgtaattt ttcaggattt tttactgtcc atgtttgaaa tgaatgattg aaataaacac
     2941 caacactgca atattgagag tgttctatgg aagaaagggt ttgattgagg ttttcctgct
     3001 ttttaatgtc aaaaaattct tcaatgatat gtgccttttc tgatggattg acatttttta
     3061 aagccctgtg cgttggtagt acaacaagcg agcgtgaaac aaggtttgtc agatatacca
     3121 tgatgtatgg cggagtatct ggatttgttt gaaaatattg gtaagacgcc tgaaatctgt
     3181 gatggccatc tgcaatgaaa agtgttgagt tttgaatttt ttcaagcagt ggcagcaata
     3241 cttcctgctt gcaggtatgt aattccacag ttgtttcttc aaaacttaat ttttcttcaa
     3301 atgccgttga gcaggcaaat ttttcaataa aatgttggtg gtcttcataa agcaaaaaaa
     3361 ttggctctaa atttgacttt gttgcttcaa gcaacctgat tctatcgtcc ttatatttat
     3421 taaaaatgac ctcatgcctg atgatgcgat cagatgcccg aaggtcaaaa agacctatga
     3481 agccagttct ttcaaggatt ttcccttcac atggaaatgt ttgtttcata agataaaaat
     3541 attcttttcc atcctgctgt aagatgccat ccttgcacca tttgttcagt gtttgagcaa
     3601 tgacactgtg ataggcagca tcaaaagatt ctggaagtgt aagatgaaca atattatatg
     3661 gtgaacgagc aataagttca gaccgatatt gctggccgat cacatcatat ggtggtgcta
     3721 tcagttgctc aagattacct gccttttttg agtatctgta ccccttaaat ggtaatatca
     3781 tgttcacagt cctgttggaa tatcaacaaa ttctgctttc acatcgaaat tttttgaaat
     3841 aacttttgcc agttcttcta ctccaagggt ttctgttgcg tgatgaccgc caaatatcac
     3901 attgatacca taatcttttg ctgagtgaaa cacttcaagg gtatcacctg aaacataaag
     3961 gtcaacgcct gcttttattg cttcattaaa cccagcataa ccaccgccac cactgataac
     4021 agcaacagtt ttaattttat ccggtccata aggaagcaac ctgcatgtta ttccaagaca
     4081 accgttgatg gtgctgacaa tatcatccag ttttttgggt tttgtgaatt gaccgcaaaa
     4141 actgatgtat tgtccgtcat atgggtaaaa gccagttgtt ttttttgcac ccatcatttt
     4201 aagaagcagg atattatttc caatttcagg gtgcatatca agtggtaggt gcgcagcgta
     4261 cagggaaatg ttatgcctgt gaagtatttc catccttttt ttgtttgcct ttgtaaatga
     4321 aggatttgca tttttccagt aatttccgtg atgcactatg ataagatttg cgttcctttt
     4381 tactgcttcc tgaaatgtat ctgctccagc atccactgtg cacatgattt tttcaacctt
     4441 ttctgtacct tcccattgga gaccattcca ggaatcatcc ttgatgttgt tgatgttcaa
     4501 gaaaccatca agaaatttca caatattatt cagtttttcc acctttcact cccagttaat
     4561 ttttactgta cttttctttc agcaacgcag gaaggtcagc gttctttaac atgtttgaaa
     4621 tatgctgttt ttttacattt ttcaattcct gtgcttccag caaaatatcc catgcctcgc
     4681 cgatgactga attttctcca tacatgaaat cgcttcccct tgaaggtgaa acaagaattg
     4741 tgcgcttgtt tttaatggct tcaacaacat ctttttcctg gcatccatca gcaaatatta
     4801 ttgttctttc tgggttggaa aaagtgccat cgtgtgtgtc agtgcttcca atcagtgatg
     4861 gaagtgtatc attctttttc cagtcgtaat agtgaaaagg aatgtgttcc cagccgtcca
     4921 ggccaatatc tgaaggaacc tcgtcaattc gttcaagttc ccacattgaa tgagtccagc
     4981 caggatgatt ccactgaata attgcagatt gctggtgggc aagagaaatg atttgttctg
     5041 gagcaagtgt ccactggatc acttttttca gcggataagc attgaaatga aaattttttg
     5101 ttgtgatttc ctgtcccact atgaacgtgt aatcaaaccc gtgttttgaa agaagtgttg
     5161 atgtaatgag cgctccatct atggtattgt ggtcagttaa tgcgagaaaa tccatacaac
     5221 aggtcattga ttgaagtgcc attccgactg gagtatgttt tccgtcagaa aaccttgaat
     5281 gactgtgcaa atctccacaa tagataaatc cctgcctttt gacattttca gatgaccttg
     5341 ttgaaaatgc aaattcattg acaagagtct ttcttatttt atcaacatcc tggatagaga
     5401 cgggttttcc tgctatcact agttttgctg cttcttcagc gacccatctg gacggatgtg
     5461 aaacagaaac aagcatatcg ttgtatggaa ttctgataag ggattgtttc acaatagata
     5521 cctggtatgc tttgaatttt tttatttcct gtggaatatc tttttctgtt gcaataagta
     5581 cactattttt tttcaatgct tcgtcgacag gaagaagatg tattttttta ctgcggatgt
     5641 atttttctac ctgttttttg attgtttcac tggcaccatc aacaattgtt gctgcttcag
     5701 cgtccacata gtcaattttt ccagtgtcca cagcatacac agttcttgat gatggtattg
     5761 caacaagtat ctgtctttcc gggtcaaatt tttctatgtt gaagtaaaaa aacatatatg
     5821 accaggaaga agcccgggca ggtagtcctg caatgacaac aggtcctgca ggattaacat
     5881 cctcaagcag gtccacaaat ccagtgattt tttcttttgc tgcttgcttt ggtaaattct
     5941 ggtctgcaaa ttttctcaca ttgacagtaa caagcggaac acttccccag taaaaagtga
     6001 tgtttgcaac ctcaggtaaa gggtactcaa caacaacctg tgattcaggg ttttcagtaa
     6061 gaacaagtaa atcaataagt gaatgagtct ttttcaattg ttttccattc aacgcattga
     6121 ttcgcgcagc gatttccctt acagcatatg ttaatttttc aacatcagca gtttcaactg
     6181 catacttgat tttctcatag catgaaccaa ctgcaaggaa tccagcggct ttgaaagggt
     6241 ctgattgaga aagttttttg atgtttgctt ttctgatttc tgaaagaatt tcttctgcaa
     6301 tgttgccaag gtaaatgaca gttttcctgc aggtgtattc agcatttttt ctgccaacta
     6361 tagcagaaat tacaaataca tcaccctgca gtttttctac atcaaaactt gctgttgccc
     6421 ttatggcttt tggccatgga ctggtgtatt taatgagttt tggttttttt tcagcaagaa
     6481 caataccctg tggattttca atcctgattt ttattgaatc aatatcacct gccattgaaa
     6541 atgtttccac tgccactggt attgattgtt tttctaacat aagtggtgaa cattcaatag
     6601 aaaaaaccgg ctgtattttt tcttcgcata cgcagtgaac aaaatgctgg ggacttctgt
     6661 aaagttcttt tttcccctga taactcaaac tcaatggctg aaccttatca aggtcctgac
     6721 tgtcgtaatc atcaagcatt gcctgaatgg caatgtgaat accagggcaa acttttttaa
     6781 tctcaggaaa tgctgaccag ggaacaaaaa gttcaatatc atagccagtt attgttttta
     6841 ctcctgctat ttgcatacct gaaattttat ttcttccaaa tacttctgcg ataggtgctt
     6901 gcctgtcaat tggtggtttg acaagaattt gataacatcc ctttgaatac ggtggtttca
     6961 taaaggagat acttcgtcca tcaacaaaaa tttcaatgca gtcctgttca tacgctgatt
     7021 ccaggggttt gtgattgatt acttcattat ccgcaacaat cgctccaaaa aacagcccct
     7081 tctcattcca ggcagcataa aattccattc ctgcgtcctg gggacctttc cattgtcttt
     7141 ttgcatttga aatgtgatac tgtgacctga tgggaaggca gaatcctgat gcccattcag
     7201 tgtcagtcaa cacgccatcc actgtgatat ttgaaaatgg aataacaaat ggtgtatcag
     7261 tggttgccgg gattgaccaa tcaacactga taccagcaaa acatgcccct gccagaacaa
     7321 agagcaataa aatttttttc attgtaattt ttaatttaac tacaggcatt tgccatttca
     7381 tttgcgattt tatgccagag ttgtctatca agatgactat ctggcgcttt taacactgac
     7441 tcaatgtgtt gtaccttttt cactcccaca atggctgatg ttactgctgg atgcgtcagt
     7501 atccatctta ctgccagttc tgccactgtg ataccaagtt tttcactgac tggcttcata
     7561 atggtattga ctgcgtttgt gatttttctg aagttttctc ctgtaaaacg gggatgattt
     7621 gcccgtgagt cctcaaacgt ttcatttcct gagtacttac ctgtgagtat tccccttgcc
     7681 aggcttgaat atgtaatgac tccaattttc ttttcaaggc agaacggaaa aagttctttt
     7741 tctgcgtctc tatcaagaat attgtatagg ggttgaaggc aatcaatatc aaaatatctt
     7801 atatacatat tcatctggtg tacattcata ttgctgacac caatccagcg gattttgccc
     7861 tgttttttca gtaaaagaag cgctgcagca acttcttctg gatttgttaa ggggtcccac
     7921 gcgtgaatct gataaaggtc aatccagtca gtttttaatc gtttcaggga cgcttcacat
     7981 tcgttgataa tatactggaa acctgtgtca ggatatctat tttcagggtc atcaatgttc
     8041 caataaaatt tggtggcaat gattaattct ttcctgcaat tatatttgct cataaaatca
     8101 ccaagcacac tttcagcaaa accatcgcca tatgcctggg ctgtgtcaac aaaattaatc
     8161 ccacaatcca ttgccttttt catagcagca ttccactcat caagagggat atttccccag
     8221 aatcttggtg acaattgcca gcaaccaaaa catattctcg agacaataag gtctgttttt
     8281 ccaagttgaa tgtatttcat atcaccctcc tgttagagag gagcaggcac ttcaaattca
     8341 atttcctttt cagattcacg tatcttaaat gacacaataa aagatacaag attgagaaaa
     8401 aggcacactg ttgtaattgt aacatacgca gggaccggat aaataatagc atcaggagtt
     8461 acaattgctt ttgatgaaaa tctatccata atgagtcctg cgatattgcc tgcaatagca
     8521 acagaaatat aacacgctcc attgacaaac cccactgctg ttccaacacc actggaagga
     8581 ccgagttctt ttatgagggt tgttgtgagg ggtccactgc cacctgtcag ggcaaaaata
     8641 aaaaagcaaa gtaaaaacac cactggtatt ttgaacaaaa taaggttggt cagcataaaa
     8701 atacaggaaa tcattgaagc aaggcataca gcgatgacaa ggactttttt ttgttttatc
     8761 agatgtacta ccagtcccca caacagtgca gcgaccatgg ttgttcccat cataatcagt
     8821 gtaaatgatg cagcaatttt tgatgataat ccacaaatat cctgaataag ttttttgccg
     8881 atgatggtct ggaaaacaaa gtaaattgtg aaattaattg cagccacaat gagcacagga
     8941 tgaatttttt tgttcagaat aactgattta atatcatcca gagaaaatga gtttttttgc
     9001 tgactgaact gatgctgttt tgcaatggtt gacaggataa ctgtcatcac aaaaaacagg
     9061 atgacgccca ttaccaagat ggtgtttctc catccaaatt gatgtacaag tcgttcaaaa
     9121 ggaagcgtgg cgaataaccc gccagaataa ccaagaaaca acgccacgcc aagcattatg
     9181 gagaaaaaat cacgaccaaa gagttcatcg gttttcttta cgagtccaag ataggcaacg
     9241 ctcgcgccaa taccaaccag tattcttgat aaataaagaa gaaaaattga atgagcaatg
     9301 gcaaacagta ttgaaccgaa acagagcaga aacccaccgc caacgaatat tctgtttgtg
     9361 ccgaagcgat caaggcaagg accgacaaaa aactgaagca atccataaat gtagaaaaca
     9421 acagtcccga gtcctgcaac ctgatttgca gtaagatgaa aatttatctg aagttcattg
     9481 aaaatcgtgc caggaattgc aaccctgtga aaataggaga aaaagtaaaa acagttgaga
     9541 agtaaaaaaa gtagcaattt ctttttcaat gacagttcca ttattaattc ctttgcgcag
     9601 gagaaaaaac aacaaaacca agtggcggaa gtactatttc aattgaaaaa tcaaaatgat
     9661 gcatcgtttg tttctctgcc tgcacaagcc cgaaatttcc tgtatttgaa ccccggtaca
     9721 aaaaagaatc actattgaga atttctttgt attcgccaga aaacggcacc ccgattctgt
     9781 attgatatct tggaacaggt gtgaaattga aaacaaacac aaggcattta tctttttttc
     9841 tattccatct taaaaacgaa acaacactgt tgtcaacatc agaaaaatca atccactgaa
     9901 aacactcatg ggactgctcg ttttcatgca tctgggggtt ttgtagataa agggtattga
     9961 ggtcttttac aagattgaat atgcccttgt ggttttcatc gttcagcagt tcccaatcaa
    10021 gttgtttcgc atggtcccat tcattccact ggccgaattc tcctgacata aataatagtt
    10081 ttttccctgg atgaccgaat tgccacccaa aaagaagccg taagtttgca aatttctgcc
    10141 agttatcgcc tggcattttt tcaacaagcg cacgttttcc atgcaccact tcatcgtgag
    10201 aaagcggaag aatgtagttt tctgaaaatg cgtacagcaa actgaaggta agtgagttga
    10261 aatggtgttt tctataaatc gggtcttttg agaaaaaact cagagtgtca tgcatccacc
    10321 ctaaattcca cttgaatcca aaaccaaggc caccaagata cacgggtttt gtgactcctg
    10381 gccatgctgt tgattcctct gctacagtaa aggagtgttt gtttctacca taaatggctg
    10441 tattcaattt ttttataaat tcaatcgctt caagattttc cctgccccca taaatatttg
    10501 gaatccattc gccttctttt cttgaataat caagataaag catagaagca acagcatcaa
    10561 cccgtaatcc gtcaatatga aagttttcaa tccagtttaa accactgccg atgagaaaat
    10621 ttgaaacctc atatcttccg taattgaaaa ttgcgctttt ccagtcagga tgttcaccct
    10681 ttttttggtc catgtgttca taaagatgag taccatcaaa ccggtaaagt ccatgactgt
    10741 ctttcgggaa atgtgctggt gtccagtcaa gaatgacacg aatattattt ttatgacagt
    10801 aatcaacgaa aaacctgaaa tcatcaggat tgccatatct tgaggttggc gcatagaaac
    10861 cggttgcctg atatccccat gatgcatcaa gaggatgttc ggcgacaggc agcagttcta
    10921 tacaattgaa attcaaagat ttcacataat caacgatttg cggagcaagt tccctgtaat
    10981 tataaaaaga accatcctgt ttccttttcc atgagcccag gtgtatttca tagatgctta
    11041 atggcttgtt taaaaaatcg gtttgctgat ttagaaattt ttcgtcttcc cagtgataat
    11101 gacttgtcca tgttattgat gcggtattgg gcctgcattc aaaatatgca ccataggggt
    11161 cagattttaa tataatggaa tggtcacggg tgagaatttc atatttataa agattgcctg
    11221 gctggaattc aggtataaaa atttcccaga taccagaatt tccaaggact ctcatctgat
    11281 gtttccttcc atcccaccta ttgaaatcgc caaccacact tacccttctt gcatcaggtg
    11341 cccagacact gaagttatag ccagaaatac cattaattgt tctatgaacc gctcccattt
    11401 ttttgaaaat ttcaaaatgg ttgccttcat taaacaggta caggtccatt tctccgaaaa
    11461 tacttgtgtt gaaggcataa gggtcaagaa aacaatattg tgttccatca ggaaattttg
    11521 cttctattct gtattgaaat ttctcatcaa agtgtccttc aaaaattccg ccttcaactg
    11581 aaataaggtt atatttttct gacgtatcaa tgttagtcac tgatacattt tttgcgtcag
    11641 gaacaaaagc gcgcaggata aaacctgtgt cagcgttttg ccaggataaa atctcatgcg
    11701 gattatggta ttcggcattg acaagtttta ataatctctc atccattttt tatcacatgc
    11761 tggaatgttg tcatggcatt tctgaacact gttaaacccc tttttgctga ctgaaatggt
    11821 cctgctgtgc caccattgat ttcataaaat acatggggaa tactgaatcg ttttgcaaat
    11881 tgatgctcaa aactattaat ccagtgagtg tcaggttgag tggaagtgga aggtgcgtct
    11941 gtgagtaatc tcatcgtatc gcacagtgcc tgatagattt ttctttttgt actgtctttg
    12001 aataattcat aagggacctc ataccatcca tcatatggat attcgctgtt ggttctccat
    12061 cctaaatagc agtgaaaatt tatccataaa tcggttcttt gttctgttgc ccacctccac
    12121 aaaagttttc cttcagtgga atctggcttg tccatatcag gattatcgct aaatacctga
    12181 taaaggtcaa ctccctcgtc agtaaaacag tttctgccag caacatttcc gtctggattg
    12241 acaatgggta tgatttcaaa aagaaagtgc tctctgagcg tttctgcctg tggaatgcct
    12301 gatgtgacaa aatcagcaat cgcccttact gcccatattc ctggaaattc gaccgggtgc
    12361 tgtcctgcga gacacagaat tcttggacct gcgggattgc cagattttat tccgaaaatt
    12421 tctctgtttc ctgctgattt tcccaaactg aaaagtgtac agttttttcg ttgttctaca
    12481 agatttttca aaaaaacaca ggtgttagaa tatgtgcatg gccagttgtt tgttattata
    12541 agttttttcc cctgtttcag ggcaagggaa atttctatat gattgtccat cgtttgacag
    12601 caggattgtt caagtggatg gaatcgtttt ccatcatcat tgctaatcca tattgttgtc
    12661 ggaaaatgag atatgaaacc ttcagggtca ttaataaccg ggtcgtgcca cacttcaatc
    12721 acaaacgaag tgtctgtgcc actattttca agttcaaagc agaagtaata gcagtaaacc
    12781 tttttatcgc cagttacctc gcatctgtat ctattctcag agatttgtga gaaatgtttt
    12841 ccatttccac actcaaaaga acttgaaaat tttatcatga attcctccta tgcggcccat
    12901 tcgtcgtcaa aactttgttt ctcaaaaaca atgtaacctg caggtggaat actgatttca
    12961 ctttcagccg ttttcattct tattaaaaga tgtacctggt cagtgccaat tttttcccag
    13021 gggattttta tttcgatgca ctcatcaagg gcgatatttt cgtctataaa agtttccttt
    13081 gtgatatcaa aagaaaaaat gatacccctg tcttctgttt caatggaaat agaagagaaa
    13141 actttctgtg atatatcaat tctgatgtat aaattgtttc tgtcatagcc ataaaaaagt
    13201 ttttttactg gaagatcaaa gggttgaaac gttgtccaga tccggatgat atcaatttca
    13261 gaagcgccgt accactcaaa gaaattcgtg attcgtccat ctatgacggc agtgatatag
    13321 ttttctggtt gtttgatgtt tgttacagga acaaatgctt ttgttacttc aaatggtacg
    13381 gataacccgg ctgtcctgta aatttttgaa ataatggact gaaacagaga gtaaaacttg
    13441 atttttgttg actgttctgc aggaacactc atccaccaga aaaaatcgct tgactcagcc
    13501 atcagggata aattgtttaa ttgttcctgt attccctgtt tactggcatg ctgttctgct
    13561 gtttttctgg catctgcgag aatttgccac agtttatttg catctggttt tccaacccag
    13621 ttgtcaaagt ttagacccat ccaggagcca gccggtattg aatcaagcga tactgtttta
    13681 aaattcagtt tgcatgcctg actgaatgta actggtttga tattaccatc agaaagtacc
    13741 ctttcgtaaa gacgggttaa aaaaacactt ccttcctgcc tgtaccactc ccaaggattt
    13801 tctccatcaa ggatgatggt aagaattttt tccctgcctt cggaaaattc ctttattttt
    13861 ttcaccaggt cttctgctgc agcgctttct tcccaggaat gatagataaa tccaattcta
    13921 tctgaaaact ggtgatttct gaaaaatata ttaaatgtat ttttccacct gtacagcgtt
    13981 gaagaaatat ccttttcctg gcaaccagaa tattgaagta tctgttcgtc tgcacccagc
    14041 cactgtatgc catattccat taaaatatca agcacttcgt tgctgataga tccttctgca
    14101 ggccagattc cattgattgt ttttccaaaa acgctgttag cagtttctat tcccattgct
    14161 atgtgcgcct ttacatcttc agggtaactg aattctatgg agggtatttc aagatgtgtt
    14221 gaagtgttcc tggcagtgga aaggtcatat acaagaggca taataggatg atagccaggg
    14281 gtggtgctga tttcaatctg acctgactga taaaggttcc tgtacagagc cagtatttct
    14341 gaaaaaactt tctgttcaaa atcaatcagg atttgtctgt ccttattgtt gaaaccaact
    14401 gctttttcgt ataattgagc gatttcaggg aaatacccca acaggaatgg gtgtatgcag
    14461 gcaaggtgca tccacatctt caagtcctga atttcagaac ccgaaatgtt ttttgaataa
    14521 aatttgtcaa tcaattttct gaaaatatta tgtctggttt ttgttctggc aggtaaatcg
    14581 tcaaggaaaa ctgaaatttt ttcagtgtca tttgcatttt catcctgcaa caatgcctga
    14641 aacccatcaa taatctggtg ttcaacgatt ttgttaatct gcagaatcaa acttggagta
    14701 aaattacagg tcagtggcat tcccgagttt tttatcatca gtgccatcgg ataataatta
    14761 taaagaaccc tgaacgcaat ggttgcattg tccattctgt ttgagtcagg cggcaaatac
    14821 aatggttgat gaaaatgcca gagaatattg agatatgtca tcgtttttca aacgccctct
    14881 ggtaaagagc aatatattgt tcaacagatt tattccagga aaaatcaagg gagattgctt
    14941 ttttgaaaag atgttccata aaagtggtgt cgggatacag gtcaatcgcc atattcattg
    15001 cttgcaccag ttgtgaaaaa tctgcgtctt caaaaacaaa tccccatccc tcctgcgttt
    15061 cgacattgat tggctgaatg gtgtctgcta atccgccggt tttccttaca ataggtattg
    15121 ttccgtattt taagctgatt aactgtccga gaccacatgg ctcaaatctt gatggcataa
    15181 ggaaaaagtc gctccctgca taaatctgac gggcaagtgc gtcgtcaaaa ccgattttga
    15241 ctgcaaccct gtcagggtat tttttcgcaa gaagcgtgat tgcctcttca tatacacgtt
    15301 cgcctgtgcc aagtcctata aactgaaaat ccttttcctt aagagattcc actgcagcaa
    15361 caattaaatc aatgcctttc tgctggacaa atcttccgac aaatccaaaa acaggtgttt
    15421 caacgctggc aggaagagaa aacttatcct gcagcatggt tttattaacc agttttcctt
    15481 ttctggttcg atactttttg aaaattgcag aatcgaaacc aggattccac acaagataat
    15541 caatgccatt gagaatgccg acaaggtctt tttctcttgt ttttaaaagg ccctcaagtt
    15601 tgcaaccaaa ttcagcgctt aaaatttctt ttgcataggt gggactgaca gtattgataa
    15661 catctgaatg aataatgcct gccttcatca gattgatgtt accgtaaaat tcaagttgtt
    15721 ccatagaaaa aaactcgcca cccagtccga gtgctggaaa ttgactggct ggaaatatcc
    15781 cctgataggc aaggttatga attgtaaaaa cgcttgcagt gccagtgtca gggtaaaaaa
    15841 gttttttgta cagtggcaga agcgctgtgt gccagtcatt gcagtgaaaa acatcaacac
    15901 tgataccaga ttgcttgcag cattcaagta ctgcctttgc aaagaaagaa aaccgtaaga
    15961 gattatcagg gtaatctata cctgattctc catacaattg atctctatca aaataatggt
    16021 tgtttgcgat caaataaaca ttgtgtttgt ctttcatcct caagagtatt gctttttcta
    16081 tcctgtcctg tgaaaaagaa acctcgtatt ccccaacaac atcacaggga tatttccggg
    16141 taatctggct gaatgctggc aggaacactt ttgtatccac atactttggg agtttgacaa
    16201 gcagtgctcc tgtaacatct gccagtcctc ctacctttac aaacggtaca cattcacaac
    16261 aaacgatcgc gacacttttc atcattctcc tttcgggtaa taatgttaaa ccacgaggtc
    16321 cctgaggaat tgcgctgctt cctcaggagg cgttggattt atgtaaaagc ctgttcccca
    16381 ctcaaagccg cctggacgtg tcagccgtgg cataatttct atgtgccagt gataatcctt
    16441 atctatggtc tgccagtatc ctggatgggg aaatctattc ggggctgtgt gaaatataaa
    16501 attatacgcc ggattgcata aacccttcat caactttttc aaaattgacc cgagtatttc
    16561 tgcaagagtt attctttcta catcactaat agaatcaaaa tcagacctgt gttttagtgg
    16621 aagaatccac agttcaaacg gaaaccttga tgcataaggc acgagggcaa taaaatcttt
    16681 attcattgcc acaacccttt caccacgact ggtttcctct tctataatat cgcagaaaat
    16741 gcatcgttct ttgtaatcat aataccattg agttcctttt aattcttctt ttactctttt
    16801 cggagttaca ggggttgcaa ttaattgtgt gtgggagtga tgaagagaag cgcctgcttc
    16861 ttttccataa ttcttgaaaa tcatgctata tttcaacctg gtatcatttt tcaagtccag
    16921 actgcgaatt tgataggtat gaatgacctt ttcaaggtgt tgcgtgtcaa aatcatgcat
    16981 ctgttgatta tgttcgggtg tttcaataat tacttcatgt gcccctatcc cgttcatttt
    17041 atcaaaaata ccaacacctt ttttatcaag attgccttca attttcaatg cgggatattt
    17101 gtttggaaca acccttacca gccatcctgg ggtatttggt tgagtattgt tatcccttat
    17161 cgcaaatatt tcaggcgggg tttttgtttc atttcccggg caaaaaggac agaatggcgc
    17221 tttctcttct cccacagttg aacacatttc agcataatct gatggcctga gttttctttc
    17281 tgtcgcgatg ataacccatc tacctataac aggatctttt ctaagttctg gcatttttct
    17341 cctctttttt aatttaccat tgataatctt ttggaatcac aacaattcct gtttcactta
    17401 cgacaaacct ttttctgtct tccacagggt cataaccaat gatagtgccc tgaggaacca
    17461 caacattctt atcaataata gttcttttta ttttacagta tcttcccaca tttactctat
    17521 ccagtaatat tgattcacta acatccgcat aactattgat tcttacccat ggagacagta
    17581 cactcctata cacccttcca ccgctgatga tgcatcctcc tccaatgatt gagtcaagaa
    17641 ccatacctgt
//
LOCUS       DAORYU010000004        16509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_2847_length_16509_cov_4.385742, whole genome shotgun sequence.
ACCESSION   DAORYU010000004 DAORYU010000000
VERSION     DAORYU010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 16509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16509
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2847_length_16509_cov_4.385742"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="PLW07_00330"
     CDS             complement(<1..653)
                     /locus_tag="PLW07_00330"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOL49003.1"
                     /translation="MASVYNVGLFGHSRSGKTILAEAVLFKAGAIDRMGDINQGNTTM
                     DYEEEEKRRLMSTNLAVAYFQWKKITCHMVDCPGYMDFIGEQISSAEAVDVGVINIAA
                     DGGIEVGTEKAWEMLKSRGLPVIIFINKLDAEQANFQSVLKQINEWLGKKAVCVTAPV
                     FAGNKIQSVVNLLDPSNQEKQEFQNLVQNLLDSIAEIDDTLMEKYLGGEQLTKDEITS
                     "
     gene            803..1381
                     /locus_tag="PLW07_00335"
     CDS             803..1381
                     /locus_tag="PLW07_00335"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOL49004.1"
                     /translation="MLNSNDEFYTTGQLAKILNCSRITVFYWIQSGKISAIKTVGGHY
                     RIPKDKIEKILKSTSTVLKKHERKTKILVVDDDPHIVKTLVLFLQKINSSYEVYGTTD
                     SFDAGRLTVTFKPDIAIIDIVMPGMDGFEIVGKIKSDPITKDTKIIAITGYGTRENIE
                     KIKKKGASLCIMKPFDYYEIAEHIKKLINPQK"
     gene            1408..2490
                     /locus_tag="PLW07_00340"
     CDS             1408..2490
                     /locus_tag="PLW07_00340"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOL49005.1"
                     /translation="MNQEKNRNNHLEKRIEFLQNQLEQAYQKIEILKTFPQSSFFEAA
                     QEWRNIFDSLSEMICLVNSQMSVIRCNIMLADFLKKPFDQIIGKKIDELFRDENLVLC
                     LKTLFLNLKQKLTRQKDIIKTEGRWYEITADPVIPVQQSDATAIFVLRDITSLKEIEE
                     YLKESHDRLKKTLHGTVSALSTTVEKRDPLTAGHERRVAALAHAIALNLGIRHEDAEG
                     ILLMGLLHDVGKIVVPAEILSRPGKLNSYEYNIVKMHPLSGHEILKKIEFPWPIAEVV
                     LQHHERLDGSGYPHGVKKDQIILEARIIAVADSIEAMLSYRPYRNRKSLDEAIHELKQ
                     LKGKHFDEQVVDACIDLFVNKHFVLE"
     gene            2529..3206
                     /locus_tag="PLW07_00345"
     CDS             2529..3206
                     /locus_tag="PLW07_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOL49006.1"
                     /translation="MNGKTVFIVEDEKDISNLIALHLRKSGFIVIEFQSSQNFFNFLK
                     NKIPDLIILDLMLPDADGLEICKYLKASVDYRSIPVIILTAKSDESDKILGLELGADD
                     YVTKPFSPRELVARVRSVLRRHNVEHTIVSIGGILVVDTQKYEVHVKNKKIHLTTTEF
                     NILKILLSKKGWVFPRETILDLLWHGEKAVIDRTIDVHIKKLREKLGEAGKFIKNIRG
                     IGYKIEE"
     gene            3203..4915
                     /locus_tag="PLW07_00350"
     CDS             3203..4915
                     /locus_tag="PLW07_00350"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOL49007.1"
                     /translation="MKRKLFFKMFLKSFLFTFCVALIVLFFFIRILKTQYLENINHKL
                     LNIVSAVETIIQTSKITKNSNDIQRVITSIDEKTGIRITIINHNGDVLADSRKNAEEM
                     ENHSDRPEFKQALEIGYGNAIRWSPTIDSYMIYCAKNIAENSIVIRASIPVDKAKIAL
                     ISQKIKILNVVLIALFFGIVFSLLLAKFATEPIYEILDFTEEIKSGRTPDIIINNKIQ
                     EIHKIMQNLTNFAGEMANFAKKEKIVSEELVKFIELVNFPIGIIDIKGDVALSNSYFK
                     EILEIPETKGLWWEKIKNFDILKIIREVNETKKTIEREIKIKDKTFLCKSTPLEEYEQ
                     ILVVLIDISTIKDIQERKKDFVIAVSHELKTPLTAIKGYIETIEENIENPENKKFLDI
                     VKHHSERLEKIVEDLITLAQLENEKPVVEFKKVDIINIANNAISLFSKKAMEKGIKIE
                     LIYNNIPEINGDAFRLEQMFINLIDNAIRFTEKGQITISMNHRNNAVIIKIADTGIGI
                     PPQHIPKIFERFYVVDKARSRQTGGTGLGLSIVKHIVLLHNGTINVESTPGKGTNFII
                     KLPA"
     gene            5034..6284
                     /locus_tag="PLW07_00355"
     CDS             5034..6284
                     /locus_tag="PLW07_00355"
                     /inference="COORDINATES: protein motif:HMM:NF024996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HOL49008.1"
                     /translation="MNEGSENARKNIENFNKGRKSMEEKEVKKCGTFASRIRMMFGGL
                     IMFMLTLLPINQAHASKEEMYILLNLLVQKGVITAEESKEMLAAVENITKKQKEDAAK
                     NSIKTSAAENIKIGGYIQGRYDSYDFSGKNDEFSLKRTRIGLSGKVIDNVSFKIEFDA
                     LKGKDNDLLTDAWIKMSYLPQFNITVGQFKLPFSEEYNISSSALDTIERSLPVNNLAT
                     EYDKGIMIDGNFIGGKIYYGVSLTNGTGGNKSDDNDNKDVTGRIVFAPWEGSENVASG
                     LKFGYGYQSGKQGTGANEKDRTRNDFMLKYQYKNLRMLAEYIGQEEKNTSTTKKNGYF
                     VQCAYNVPLNNGKSIEPVVKYEVYDPNTKVSKNTQTIFTAGFNLYLNKLTKLSTNYRW
                     RNDDQGGNIASNKNEWFTQLQIKF"
     gene            6315..7361
                     /gene="pstS"
                     /locus_tag="PLW07_00360"
     CDS             6315..7361
                     /gene="pstS"
                     /locus_tag="PLW07_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007568943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HOL49009.1"
                     /translation="MKKFKKFIAGIAGLMLIFCASIKASDHEITGAGATFPYPLYSKM
                     FDVYNKEQGIKINYSAIGSGGGIRQLMAKTVDFGGSDAFLTDQQLKEMGADVVHIPVC
                     MGAVVLAYNLPGISELNLTPELIADIFLGKIKKWNDPRLQKANPKAKLPDANLVTAHR
                     SDGSGTTFIFTDYLSKISAEWKTKIGTGTSVNWPSGLGGKGNPGVAGLIKQIPYSIGY
                     VELIYAISNKIPYATIQNKSGVFVKPSLTSTSIAGDIKLPYDMRISITDTDAKEGYPI
                     SGFTWIIVYKEQNYNNRSYDRAKNLVNILWWMTHQGQKYTTELHYAPLPKQAVMKAEN
                     LIKSITYNGKRIIN"
     gene            7394..8365
                     /gene="pstC"
                     /locus_tag="PLW07_00365"
     CDS             7394..8365
                     /gene="pstC"
                     /locus_tag="PLW07_00365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOL49010.1"
                     /translation="MANIKPSEHKTQQVCHKPDPGKHSRVRWFFNNQHPIPDFLFKTI
                     LLFSGILIFVLTLSIFLSLVINSIPSIKMFGFKFFTGKIWDPVFEQFGALPFILGTLL
                     TSFLAILITIPFSIAISIYLGEFSKTGMYANFLKNSIDLLAGVPSVVYGFFGIFVIVP
                     AVRYLQIKSGIDPYGVGIFTASIVLSVMIIPFSASLGREVIEMIPSEIKEAAYALGST
                     RYEVARHLILPYARSGLIAGILLALGRAIGETMAVTMVIGNSNQIPKTIFAPANTMAS
                     IIANEFAEAVTTIHLSSLIEIGIVLFIVTTIVSILGKIIIRKFAVHV"
     gene            8368..9210
                     /gene="pstA"
                     /locus_tag="PLW07_00370"
     CDS             8368..9210
                     /gene="pstA"
                     /locus_tag="PLW07_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOL49011.1"
                     /translation="MEKLKKRILQDRVFTILIIVFAFTACLPLLGILAFIVKNGITVI
                     NFEFFFHLPKPVGEHGGGISNAIVGTFIVVMIAFIIASPVSLLMGIYLSENRKSLFSE
                     ILRVCIDVLASIPSIVIGIIAYLWIVKPMKSFSALSGGIALAIIMLPVVVKSTEETLN
                     MIQSTLREASFALGVPYFRTILKVIVPAGLSGIITGILIALARIAGETAPLLFTAFGN
                     PFMNFNITKPVNTLPLLVFNYSTSPYQQWHTIAWGAALVLLIIVFTFNILARIISKKW
                     KTNF"
     gene            9192..9950
                     /locus_tag="PLW07_00375"
     CDS             9192..9950
                     /locus_tag="PLW07_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HOL49012.1"
                     /translation="MENKLLIVENFSAGFDKCNILNNINFAVHQNTVTAIMGPSGCGK
                     TTMIRCINRMHEFSQGATYSGKILLHGDDIYSMNPILVRRKIGMVFQRPNPFPTMSIY
                     DNVISGFILNGIKLSKQKKDAIVIDTLKKVGLWDEVKDSLHRRGSFLSGGQQQRLCIA
                     RALAMNPDIILLDEPTSSLDPKSTLMIEQLVEQLKQTTTIIIVTHNTAQAARISDYVA
                     FLYLGELVEYGPTKKIFTAPEHKKTEEYLTGKFS"
     gene            9960..10613
                     /gene="phoU"
                     /locus_tag="PLW07_00380"
     CDS             9960..10613
                     /gene="phoU"
                     /locus_tag="PLW07_00380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOL49013.1"
                     /translation="MIENRINETKQKLIEFSLLVENMIAESIDGLLKKNASILKKIIE
                     GDEAIANKLEIEIEEMCIEIIAQYSPKASYLRTTLMILKINNDLERIGDHAVNIAQSA
                     LFLIERPDVKPLIDTPRMAEEAAGMLKNSLKSFINQDAELAKSVCRRDEIVDRLRDQI
                     ARELITYMISDPSTIERSLQLLRISQNLERIADLSTNISEDVVFMVEGKIIKHHSTE"
     gene            complement(10618..10733)
                     /gene="rrf"
                     /locus_tag="PLW07_00385"
     rRNA            complement(10618..10733)
                     /gene="rrf"
                     /locus_tag="PLW07_00385"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(10756..11790)
                     /gene="mtnA"
                     /locus_tag="PLW07_00390"
     CDS             complement(10756..11790)
                     /gene="mtnA"
                     /locus_tag="PLW07_00390"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOL49014.1"
                     /translation="MEQIIKTLSWKKSFLEIMDQSRLPSQEKYIKLFTVNQVYQAIKT
                     LKIRGAPAIGLAAAFGLLLATKQMKTKNKDKFFYLIQQKAEYLKGCRPTAYNLFYGID
                     RLIDVAKQHLHLSIEEIIMTMEKECLNMFYEDLQNSFKIAEYGEKLIKDGMRILTHCN
                     AGALATSGIGTALAPIYLAAKRNKKVEVFVDETRPVLQGARLTMWELKKAKIKCTLVC
                     DNMAGFLMQQKKIDIVITGADRIARNGDTANKIGTYSVAVLAKFHDIPFYIAAPLSTF
                     DFSLPDGNAIPIEQRNKKEIEYIQGKRIAPLNINVYNPAFDVTPCNLITGVITEKGLF
                     RADRIKNLTR"
     gene            complement(11792..15214)
                     /gene="dnaE"
                     /locus_tag="PLW07_00395"
     CDS             complement(11792..15214)
                     /gene="dnaE"
                     /locus_tag="PLW07_00395"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOL49015.1"
                     /translation="MKDFVHLHLHTEYSLLDGMCRIDEVTKLAHQYGMSGVAITDHGG
                     LFGVIPFYESALNHGIKPIIGCEMYMSSGSRFEKKSTDKRESAYHLTVLAINNKGYEN
                     LIELSTLSYLEGYYHKPRIDRELLSKLSDGLIVLSGCLQGEINQLLLSGNQDRAISAL
                     NWYLDVFGKENFYIELMENGIPEQTKANRMVIDIARKNGIKIVATNDCHYLRKNDVFT
                     HEILLCIQTGTNVEDENRLKFSGSEFYFKSPEEMQLLFKEIPEATKNTVEIFEKCNVT
                     FDFSTHHIPRFPVPEGMSDAEYLEKLVFNGLQRQFGMTIDQDNEITKRVRYELDVIKK
                     MNFSGYFLIIQDIVNEAKKRKIAVGPGRGSGPGSLVAYLLGITEINPLKYDLLFERFL
                     NPDRISMPDIDLDFDDTKRDQVIDYIRQKYGEKNVAQIATFGTMGARAVVRDVGRALR
                     MSYTEVDRIAKLISPEPGALLKEELVKNEEIKRLIGADERVKKLFDISLSLEGLARHA
                     STHAAGVVITDIPTYKYVPLFRGTRGEVSSQFDMTGIEKIGLLKIDILGLKNLSMIEE
                     AVKLVKIHKKVEITEFPENDKKSYDMLCKGESIGLFQMESQGMQELLRRIKPRVFEDI
                     IAILALYRPGPMQSGMVEQYIQYKKDPSKIRYDHPLMEPVLKTTYGVILYQEQVMKLA
                     SVIGSFTMAEADILRKAMGKKDPELLEKQREKFLKGAKQKGIRQDVAEKIFNNMAKFA
                     GYGFNKSHSTCYAMIAYQTVYLKANYPLEYMTALLNSEIGNEEKIRMYIEEAERMNIW
                     VLPPDIQASDEKFSIVGNDILFGLSAIKNVGQGAIQSIIEARKQGKFESLFDFCQRVD
                     LRLINRKVIESLIKAGAFDSFEKPRSQLYALMDEAIEQGSRLQKLNVEGQLSIFSKTE
                     KFIPPSIHDAISELPEWPESKLLAFEREMLGVYRTGHPLENCRETLSHFVNAWSNQVD
                     ELAEGKEYWFGGLIQNLKRKTSRKGEKMAIGELEDLYGKIEVVFYPQIYENISSLLKV
                     SNIVFVKGKVEHRSERTTIVAKDVATINTVAEKCAKSLTITFGLETSRNVLSQLKEIF
                     ESHRGNSIVYLVINDNNGGHIKLKLSKFQVALVPELFDKLKQVFPEQNISLN"
     gene            complement(15211..16374)
                     /locus_tag="PLW07_00400"
     CDS             complement(15211..16374)
                     /locus_tag="PLW07_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HOL49016.1"
                     /translation="MGMWIGRGRKARVCYGFDDISLVPGSLTINPAEIDISVIIAGKR
                     LEVPILAAAMDGVVDPKFAIAFGKTGGLAVFNLMGIYTRYDDPYSVIEEIIACPQEKA
                     TALIQKIYHEPVKEHLIEKRIQEIKDGGVLCAVSSIPQHAERFGAIAQSAGADIFVVQ
                     STVTTAKHISSTYKPLDFFEFCSSMKIPVCAGNCVTYQVALELMETGISGLLVGIGPG
                     AACTTREVLGIGVPQVAATVDCAAARDFFYKRTGKYVPIITDGGMITSGDICKAFACG
                     ADAVMVGSAFVRANEAPAKGYHWGMATSDENLPRGTRIRLGTTGPVKQILFGPSNTDD
                     GSQNLIGALRLSMASLGARNIRELQLVEIAIAPSIKTEGKIYQVAKKQAMIGK"
BASE COUNT         5451 a         3168 c         2882 g         5008 t
ORIGIN      
        1 gatgtgattt catcttttgt aagttgttct ccaccgagat atttttccat cagggtatca
       61 tcaatctcag cgattgaatc aaggagattt tgcacaagat tttgaaactc ctgtttttcc
      121 tggtttgaag ggtcaaggag attcacaaca gattggattt tgttaccagc gaacacagga
      181 gcagtcacgc atactgcctt tttaccaagc cattcgttta tttgtttcaa aactgattga
      241 aaattcgctt gttctgcatc aagtttgttg ataaagataa ttactggcaa tcccctgctt
      301 ttcaacatct cccatgcctt ttctgtacca acttctatgc caccgtctgc agcaatattg
      361 ataacaccaa catccactgc ctctgcagaa gaaatctgct ctccgataaa gtccatataa
      421 cccggacaat caaccatatg acaggttatt tttttccact gaaaatacgc aacagcaaga
      481 ttggtactca tcaatcttcg tttttcttcc tcctcatagt ccatggtggt attgccctga
      541 ttgatatcac ccatcctgtc tattgcacct gccttaaaca gtactgcttc agcaagaatt
      601 gtcttgcctg aacgactgtg tccaaataat ccaacgttat aaacagatgc caccaatcac
      661 ctccatcaaa ttgaaacaga gaatttgctt tgtgattatt gtatcacaaa aattattttg
      721 ataaaacatt ttgtagtaaa gtggcaaaac catcccttga cagggaaaaa tataatgtta
      781 aaattgtcag aaagggtaaa aaatgttaaa ttcaaacgat gaattttaca caacaggaca
      841 acttgcaaaa attttgaatt gttcgcggat aacagttttt tactggatac agtcaggaaa
      901 aattagcgcc atcaaaacag tgggtggtca ttaccgtatt ccaaaggaca aaatagaaaa
      961 aattttgaaa tctacttcaa cggtgcttaa gaaacatgaa agaaaaacaa aaattctggt
     1021 cgttgacgat gaccctcata ttgtaaaaac ccttgtttta tttcttcaaa aaataaactc
     1081 ttcatacgaa gtttatggaa caacagatag ttttgatgca ggacgactga cggttacatt
     1141 caaaccagat attgccatta ttgatattgt aatgccgggg atggatggat ttgaaatagt
     1201 tggaaaaatt aaatcagacc caatcacaaa agatacaaaa attattgcca tcacaggcta
     1261 cggaacaaga gaaaatattg aaaagataaa aaagaaaggt gcaagtttat gtataatgaa
     1321 accctttgat tattatgaga ttgctgaaca tataaaaaaa cttatcaacc ctcaaaaata
     1381 atcaaaacgg ccttcataaa agttaatatg aaccaagaaa aaaatagaaa taaccatctg
     1441 gaaaaaagga ttgagtttct tcaaaaccaa cttgagcaag cctatcaaaa aattgaaatc
     1501 ctgaaaacat tcccgcaatc atcatttttt gaagcagccc aggaatggag aaatatcttt
     1561 gattctcttt ctgagatgat atgtcttgtt aacagccaga tgtcagtcat tcgatgcaat
     1621 attatgctgg cagatttttt gaaaaagcca tttgaccaga ttatcgggaa aaaaattgat
     1681 gaactttttc gggatgaaaa tctcgttttg tgtttaaaaa ctcttttctt aaatttgaaa
     1741 cagaaactca caaggcagaa agatataata aaaaccgagg gaagatggta cgaaatcact
     1801 gctgatcccg taataccagt gcaacaaagt gatgcgactg ccatttttgt tctcagggac
     1861 attacctctc tgaaagaaat cgaagaatat ctcaaagaaa gtcatgatag attgaagaaa
     1921 acattgcatg gtacggttag tgcactttcc acaacagtgg aaaaaagaga cccccttact
     1981 gcaggtcatg aaagaagggt ggcagcactt gctcatgcca ttgcgctgaa cttagggata
     2041 agacatgaag atgccgaagg cattttactt atggggcttc ttcacgatgt aggaaaaatt
     2101 gttgtgccag cggaaatttt aagcaggcca gggaaactga attcatatga atataacatt
     2161 gtaaaaatgc atccattatc aggacatgaa atactgaaaa aaatagaatt cccatggccc
     2221 atagcagaag ttgttcttca gcaccatgaa cgattggatg gttcagggta tcctcatggt
     2281 gtaaaaaaag accagattat tcttgaagca agaattattg ctgtcgctga ttccattgaa
     2341 gcaatgcttt catacagacc ttacagaaac cgaaaatccc ttgatgaagc gatccatgaa
     2401 ctgaaacagt tgaaaggtaa acattttgac gagcaggtcg ttgatgcctg cattgacctt
     2461 ttcgtaaaca aacactttgt gcttgaataa ttctttttat tacctgcctt tcagattata
     2521 ataactgtat gaatggcaaa actgttttta ttgttgaaga cgaaaaagac atatccaatc
     2581 tgattgctct gcatttgaga aaatcaggat ttattgttat tgaatttcaa tcatcccaaa
     2641 actttttcaa tttcctgaaa aataaaattc ctgacctgat tattcttgat ttaatgctac
     2701 ccgatgcaga cggtttagaa atctgcaagt atctcaaagc aagtgttgat taccgctcca
     2761 ttccagttat cattctcact gcaaaatccg atgaatctga caaaattctt ggccttgaat
     2821 taggagcaga tgactatgtt accaaacctt tttctccaag agagctcgtc gcaagggtaa
     2881 gaagtgtgtt aagacgccat aatgttgaac atacaattgt ttctatcgga ggtattcttg
     2941 tagtcgatac tcaaaaatat gaagttcatg taaaaaataa aaaaatacac ctgaccacaa
     3001 ctgaatttaa cattcttaaa atcctgttga gcaaaaaagg gtgggttttc ccgagggaaa
     3061 caattctcga cctgttgtgg cacggagaaa aggcagtaat cgatagaacc attgatgtgc
     3121 acataaaaaa actgagagaa aaacttggag aagcaggtaa atttatcaaa aatatcaggg
     3181 gtattggtta caaaatagaa gaatgaaaag aaaactattt ttcaaaatgt ttttaaaatc
     3241 ctttctgttc actttctgtg ttgcattaat tgttttgttt ttctttatcc gcatactgaa
     3301 aacacaatat cttgaaaata ttaatcacaa acttttgaat atagtaagtg ccgttgaaac
     3361 aattattcag acatcaaaaa tcacgaaaaa ttctaatgat attcaacgcg tcataacaag
     3421 tattgatgaa aaaactggca taagaataac gattatcaat cacaatggag atgttcttgc
     3481 tgactccaga aaaaatgctg aagaaatgga aaatcattca gatagacctg aattcaaaca
     3541 ggcacttgaa ataggatatg gcaatgccat cagatggagt ccaacaattg attcatatat
     3601 gatatattgc gcaaaaaaca tcgctgaaaa tagcattgtt atcagggcga gtattcccgt
     3661 agataaagca aaaatcgcat taataagcca aaaaatcaaa atcctgaatg tggttttgat
     3721 agcattattt tttggaattg ttttctcgct tttacttgca aaatttgcga cagaaccaat
     3781 ttatgaaatt cttgatttca ccgaggaaat aaaatccgga agaacccctg atataattat
     3841 taataataaa atccaagaaa tccataaaat catgcaaaat ctaactaact tcgctggtga
     3901 aatggcaaat ttcgcaaaaa aagaaaaaat tgtttctgaa gaactggtca aatttataga
     3961 acttgttaac tttcctatag gcatcataga cataaaagga gatgtagcac tatcaaattc
     4021 ttacttcaaa gaaattcttg aaataccaga aacaaagggc ctctggtggg aaaaaataaa
     4081 aaactttgat attctcaaaa taataagaga agtaaacgaa acgaaaaaaa caatcgaacg
     4141 ggaaatcaaa ataaaagaca aaaccttttt atgtaaatcc acaccactgg aagaatatga
     4201 acaaattttg gttgttctta ttgatatttc aacgataaaa gacatacagg aaaggaaaaa
     4261 ggactttgta attgctgtct cacatgaatt gaagacccct ttaaccgcaa tcaaaggtta
     4321 cattgaaaca atagaagaaa acattgaaaa cccagagaat aaaaaatttc ttgatatagt
     4381 gaaacatcat tcggaaaggc ttgaaaaaat tgtagaagac ctcatcacac tggctcaact
     4441 tgaaaatgaa aaacctgttg ttgagtttaa aaaagtggac atcattaaca ttgcaaataa
     4501 tgccatatca cttttttcaa aaaaagcaat ggaaaaaggt ataaaaatag aacttatcta
     4561 taacaatatc ccggaaatta atggagatgc attccggctc gaacaaatgt tcatcaatct
     4621 tattgacaat gcaataagat tcacagaaaa aggtcaaatc actatttcca tgaatcatcg
     4681 aaacaatgct gtgatcataa agattgcaga cacaggtatt ggtattccac cacagcatat
     4741 accaaaaatt ttcgaacggt tctatgtcgt tgacaaagcc agaagcagac aaactggtgg
     4801 tactgggctg ggactttcca ttgtaaaaca tatagtactt ttgcataatg gaacaataaa
     4861 tgtagaaagc acgcctggca aaggaaccaa ctttattatt aaactgcctg cctgaaaatc
     4921 tacactttca atcagcgtaa aaaattaaca caattttaac aattccttta ttttttcttt
     4981 aaaaattacg gctactatat ctgtgtaaaa aaatttttca acaaggaggc aatatgaatg
     5041 aaggcagtga aaatgcaaga aaaaatattg aaaattttaa caaagggagg aaaagtatgg
     5101 aagaaaaaga agtaaaaaaa tgcgggactt ttgcaagtag aataagaatg atgtttggtg
     5161 ggttgataat gtttatgcta acgttgttac caatcaacca ggcgcatgca agtaaagaag
     5221 aaatgtatat acttttaaac ttgcttgttc aaaagggtgt tatcactgct gaagaaagta
     5281 aggaaatgct ggctgcagtt gaaaatatca caaaaaaaca aaaagaagat gctgcaaaga
     5341 attcaataaa aacttctgct gctgaaaata taaagattgg cggttatatt cagggaagat
     5401 atgattctta cgatttctca ggcaaaaatg acgaattttc actcaaaaga acaagaatag
     5461 gtctctcagg caaggtaatt gacaatgttt ccttcaagat tgaatttgat gcattaaaag
     5521 ggaaagataa tgacttgctc acagacgcgt ggataaaaat gtcatatctt ccgcagttca
     5581 atattacggt tggacagttt aaacttccat tttcagaaga atataacatc tcatcatccg
     5641 cgcttgacac aatagaaagg tctcttccag tcaataacct ggcaacagaa tatgacaaag
     5701 gtatcatgat tgatggaaat ttcattggtg gaaaaatcta ttatggtgtt tctctgacaa
     5761 atgggactgg aggaaataaa tctgacgaca acgacaataa agatgttact ggaagaattg
     5821 tgtttgctcc atgggaagga agcgaaaatg tagcttcagg acttaaattt gggtacggtt
     5881 atcaatccgg caaacagggt actggagcaa atgaaaaaga cagaacaaga aatgatttta
     5941 tgttgaagta tcaatataaa aatttaagaa tgctcgcaga atatatcggc caggaagaaa
     6001 aaaatacatc aacaaccaaa aagaatggtt attttgtaca atgcgcttac aatgtaccac
     6061 tcaataatgg taaaagtatt gaaccagtcg tcaaatatga agtgtacgac cctaatacaa
     6121 aagtatcaaa aaacacacaa acaattttca ctgctggctt taatctttat ctaaacaaac
     6181 ttaccaaact cagcaccaac tatagatgga gaaatgatga ccagggtgga aatattgctt
     6241 ccaataaaaa tgagtggttc acacaactgc agataaaatt ttaacaacat tataacaaaa
     6301 aagggaggat gaatatgaaa aagtttaaaa aattcatagc aggaatagct ggattaatgc
     6361 tgattttttg cgcgtcaatc aaagcatctg accatgaaat cactggcgca ggtgcaacat
     6421 tcccgtatcc tttatactcg aaaatgtttg atgtctataa caaagaacag ggaataaaaa
     6481 tcaactactc agcaattggc tctggcggtg gaataagaca gttaatggca aaaacagttg
     6541 atttcggcgg ttcagacgca tttcttactg accagcaact taaagaaatg ggtgctgacg
     6601 tagtacatat cccagtttgt atgggtgctg ttgttcttgc atacaatctt ccaggtattt
     6661 ctgaattaaa tcttactcca gaattgattg ctgacatatt tcttggcaaa attaaaaagt
     6721 ggaacgaccc aagattacaa aaagcaaacc ctaaagcaaa gcttcctgat gcaaatttgg
     6781 tcactgctca tagatcagat ggaagtggaa caacttttat tttcactgat tatttgagta
     6841 aaatcagtgc tgaatggaaa acaaaaattg gaacaggaac ttctgtgaac tggccatccg
     6901 gtcttggtgg aaaagggaac cctggcgttg caggacttat taaacagatt ccatattcta
     6961 ttggatatgt ggaacttatt tatgctattt caaacaaaat tccatatgcc acaatccaga
     7021 acaaaagtgg cgtgtttgta aaaccatccc ttacaagcac aagcattgct ggagatatta
     7081 aacttcctta tgatatgcga atatccatca ctgatactga tgcaaaagaa ggttacccaa
     7141 ttagtggatt cacatggatt atcgtttata aagaacagaa ctataataac agatcttatg
     7201 atagggcaaa aaatcttgtt aatattttgt ggtggatgac ccatcaggga cagaaataca
     7261 cgacagaact ccattacgct ccacttccaa aacaggcagt tatgaaagca gaaaatttaa
     7321 taaaatccat cacttacaat ggaaaaagaa taataaattg aaagaataat gacattcagg
     7381 taaaataata taaatggcaa atataaaacc ttcggaacat aaaactcaac aagtgtgtca
     7441 caaacctgac cctggaaagc actccagggt caggtggttt ttcaataatc agcaccccat
     7501 acctgatttt ttgtttaaaa caattctttt attttctgga atactcattt tcgtgctgac
     7561 attatccata ttcttgtcat tggttatcaa ttccattcct tcaattaaaa tgtttggttt
     7621 taaatttttt actggtaaaa tctgggatcc tgtgtttgaa caattcggag cattaccctt
     7681 catccttgga acccttttaa cttcattttt agcaattctc atcaccattc cattttctat
     7741 agcaatatca atatatcttg gagaattttc aaaaacaggt atgtatgcta attttttgaa
     7801 aaacagcata gatttacttg ctggcgtgcc atctgttgta tatgggtttt ttggcatatt
     7861 tgtcattgtc cctgctgtaa gatatcttca gataaaatct ggcattgacc cttatggagt
     7921 cgggatattc actgcttcaa ttgtgctttc tgtaatgatt atcccatttt cggcttcgct
     7981 cggaagagaa gtaattgaaa tgataccctc agaaatcaaa gaagccgcat atgctctcgg
     8041 aagcacaaga tacgaggtgg caaggcatct catattacca tatgccagaa gcggtttaat
     8101 agcaggtatt ttacttgcgc ttggaagagc cataggcgaa acaatggctg taacaatggt
     8161 aataggaaat tcaaatcaga taccaaaaac aatttttgcg cctgcaaata caatggcatc
     8221 catcatagca aacgaatttg cagaagcagt taccaccatt catctttcaa gtctcattga
     8281 aataggcatt gttctgttta ttgtcacaac tattgtcagc attttaggaa aaattattat
     8341 cagaaagttt gctgtgcatg tgtaattatg gaaaaactta aaaaaagaat tttgcaggat
     8401 agagtgttta cgatactgat aattgtgttt gcgtttactg cctgcttgcc attgctggga
     8461 attcttgctt ttattgtaaa aaatggaatt acagttatca attttgaatt cttttttcat
     8521 cttccgaaac ctgttggaga acatggtggt ggtatttcaa atgccattgt tggaactttt
     8581 attgttgtaa tgattgcttt tattattgca agtcctgtat cgttattaat gggtatttat
     8641 ctgtcagaaa acagaaaaag tttgttttct gaaatattac gggtatgcat tgatgtgctt
     8701 gcaagtattc cttcaatagt tattggaatc attgcctatt tgtggattgt aaaaccaatg
     8761 aaatcatttt cggctctatc aggaggcatt gctcttgcaa ttattatgtt acctgttgtt
     8821 gttaaatcta cagaagaaac cttaaacatg atacaatcaa ccttgagaga agcttctttt
     8881 gcgcttgggg taccctattt cagaacaatt ttaaaagtga ttgtgccagc gggtctcagt
     8941 ggaataatca caggtatcct tattgcgctc gctcgaatag caggggaaac tgctccttta
     9001 ttattcactg catttggtaa tccctttatg aacttcaata tcacaaaacc agtaaataca
     9061 ttgcccttac ttgttttcaa ttactcaacc agtccatatc aacaatggca tacaattgcc
     9121 tggggcgcgg ctcttgtact gttaatcatt gtttttacct ttaacatact tgcaaggatc
     9181 atatcaaaaa aatggaaaac aaacttttaa ttgtcgaaaa tttttctgcg ggttttgata
     9241 aatgtaatat actcaacaat atcaactttg ctgttcatca aaacacagtc accgcaatca
     9301 tgggtccatc aggctgtgga aaaacaacaa tgatcagatg catcaataga atgcatgagt
     9361 tctcgcaagg agcaacatat tcaggaaaaa ttctgttgca cggtgatgat atctattcaa
     9421 tgaatccaat acttgtaaga agaaaaattg gaatggtatt tcagagacca aacccatttc
     9481 ctaccatgag tatatacgac aatgtcattt ctggtttcat ccttaatgga ataaaattga
     9541 gcaaacaaaa aaaggatgca attgttattg atactttaaa aaaggttggt ctctgggatg
     9601 aagtaaagga ttctcttcac aggcgtggta gttttctttc aggaggacag caacaaagat
     9661 tatgtattgc aagggcactg gcaatgaatc ctgatattat ccttcttgac gaacccacat
     9721 catcccttga tccaaaatca acccttatga ttgaacaact ggtagaacag ctgaaacaaa
     9781 caacgacaat tatcattgtc acacataata ctgcgcaggc agcaagaatt tctgattatg
     9841 ttgcattttt atatctcgga gaattagttg aatatggtcc gacaaaaaag attttcactg
     9901 cgcctgaaca taaaaaaaca gaagaatatc tgacaggcaa attcagttaa ggagatatca
     9961 tgatagaaaa cagaattaac gaaacaaaac agaaacttat tgaattttca ttgctggttg
    10021 agaatatgat tgcagaaagc atagacggtc ttttaaaaaa gaacgcctct attcttaaaa
    10081 aaatcataga aggtgatgaa gcaatcgcaa ataaattgga aatagaaatc gaagagatgt
    10141 gcattgaaat tattgcccaa tattctccaa aagcatccta tttaagaaca accctgatga
    10201 ttctaaaaat caataatgac cttgagagaa taggagacca tgcagtaaat attgctcaga
    10261 gcgcattgtt tttaattgaa agacctgatg tgaaaccatt aatagatacc ccgagaatgg
    10321 ctgaagaagc agcaggcatg ttgaaaaata gtttaaaatc tttcatcaat caggatgctg
    10381 aactcgctaa aagtgtttgc agaagagacg aaattgttga tagattgaga gaccagatcg
    10441 cgagagaact catcacatat atgatttcag atccttctac aatagaaaga tccttgcaac
    10501 ttcttcgcat atctcaaaat cttgaaagaa ttgcggattt atcaacaaac atcagtgaag
    10561 atgtggtttt tatggttgaa ggtaaaatta taaagcatca ttccacagaa taaaaaatcc
    10621 cggcaacacc tactctcccg cccgctctcg cggacagtac catcggcgcc agagggctta
    10681 acgaccgtgt tcgggatggg aacgggtgtt tcccctcggc catggttgcc gggaatataa
    10741 taataccatt tttttttatc ttgtcaaatt ctttattcta tctgccctga ataatccttt
    10801 ttcagtaata actcctgtga ttaaattgca aggcgtaaca tcaaaagcag gattataaac
    10861 attgatattg agcggcgcaa tccttttccc ctggatatac tcaatttctt ttttattcct
    10921 ctgctctatt ggaatggcgt taccatcagg caaggaaaaa tcaaatgtcg aaagtggcgc
    10981 agcaatataa aatggtatat catggaattt tgcaagcact gcaacagaat aagtgccaat
    11041 tttattggcg gtgtcaccat ttcttgcaat tctatcagcc cctgtaatta caatgtcaat
    11101 ctttttttgc tgcataagaa accccgccat attgtcgcat acaagggtgc attttatttt
    11161 tgcttttttc aactcccaca tggtaagtcg tgctccctgt aatacaggcc ttgtttcatc
    11221 cacaaatact tccactttct tatttctttt tgccgcaaga taaatcggag caagcgctgt
    11281 tcctatgcct gatgttgcca gggcaccagc attacaatga gttaatattc tcattccatc
    11341 cttaataagt ttttcaccat attctgctat tttaaaactg ttttgaaggt cttcgtaaaa
    11401 catgttcaaa cactcttttt ccatcgtcat tatgatttct tcaatagaaa gatgcaggtg
    11461 ctgttttgcg acatcaatca acctgtcaat accgtaaaac aggttgtagg cagtaggcct
    11521 gcaacccttc aaatattccg ccttctgctg gatcaaatag aaaaacttgt ctttgttttt
    11581 tgtcttcatt tgttttgttg caagaagcaa tccaaacgca gcagcaagtc ctatcgcagg
    11641 agcacctcta atttttaaag ttttaattgc ttgatacacc tgattcactg taaaaagttt
    11701 gatgtatttt tcctgagaag ggagccgcga ctgatccata atttcaagaa aacttttctt
    11761 ccaggaaagt gtctttataa tctgttccat attaatttaa agaaatattc tgttcaggga
    11821 aaacctgttt tagtttgtca aataattcag gaacaagtgc aacctgaaat ttgcttaatt
    11881 tcaattttat gtgaccgccg ttattatcgt ttataacgag atacacaatg gagttgcctc
    11941 tgtgagattc aaaaatttct tttaattgag aaagcacgtt tctggaggtc tctaaaccaa
    12001 atgttatcgt caaacttttt gcgcattttt cggcaactgt attaattgtg gcaacatcct
    12061 ttgctacaat ggttgttctt tcagacctat gttccacttt gccttttaca aaaacaatat
    12121 tactcacctt cagaagactt gaaatgtttt catatatttg cggataaaaa acgacttcta
    12181 ttttaccgta aaggtcttca agttctccta tagccatctt ttcccctttt ctactggttt
    12241 tacgtttgag attctgtatc aacccaccaa accagtattc ttttccctca gcaagttcgt
    12301 caacctgatt actccatgca tttacaaagt gcgaaagcgt ctcacgacaa ttctcaaggg
    12361 gatgtcctgt tctataaact cccagcattt ctctttcaaa tgcaagaagt tttgattccg
    12421 gccactcagg aagttcagaa attgcatcat gtattgatgg cggtataaat ttttctgttt
    12481 ttgaaaaaat tgaaagctgt ccctcaacat taagtttctg caatctacta ccctgttcta
    12541 ttgcttcatc cattaatgca tacagctgag atcttggttt ttcaaaagaa tcaaaagcac
    12601 ctgccttaat aaggctctct ataacctttc tattgattaa cctgaggtca accctctggc
    12661 aaaagtcaaa aagggactca aatttaccct gttttctggc ttcaataatt gattgaattg
    12721 ctccctgccc aacatttttt attgcagaca aaccaaacag tatatcgttt ccaactattg
    12781 aaaatttttc atcgcttgcc tgtatatctg gtggaagaac ccatatattc attcgttcag
    12841 cctcttcaat atacattcta atcttctctt cattacctat ttcgctgttc aacagggctg
    12901 tcatatattc aagaggataa tttgccttca gatatactgt ttgatatgca atcattgcat
    12961 aacaggtact atgagattta ttaaaaccat accctgcaaa ctttgccata ttattgaaaa
    13021 ttttctctgc gacatcctga cgaatccctt tctgttttgc acctttaagg aatttctctc
    13081 tctgtttttc aagtagttca gggtctttct tgcccattgc cttgcgcaga atgtctgcct
    13141 cagccatcgt gaaacttcca atcacactgg caagtttcat tacctgttcc tggtagagaa
    13201 tcactccgta agtggtctta agaacaggtt ccatcaaagg atggtcgtat ctaatttttg
    13261 atgggtcttt tttgtattga atatattgtt caaccattcc actttgcatt ggtcctggtc
    13321 tatatagcgc aagtattgca ataatgtcct caaataccct tggtttaatt cttcttaaca
    13381 gttcctgcat accctgactc tccatctgaa ataaaccaat ggattctcct ttacacagca
    13441 tatcataaga ttttttatcg ttctcaggga attctgtaat ttccaccttt ttatgaattt
    13501 taaccagttt gacagcttct tctatcattg aaagattttt taacccgagg atgtctatct
    13561 tcaaaagccc tattttttca atacccgtca tgtcaaactg acttgatacc tcacccctgg
    13621 ttccgcgaaa tagaggaaca tacttatatg ttggaatatc agtaataaca acgcctgccg
    13681 catgggttga ggcgtgcctt gccagaccct caagagaaag tgaaatgtca aacagctttt
    13741 ttactctttc atctgccccg atgagtcgtt tgatttcttc attctttacc aattcttctt
    13801 tcagcaatgc tccgggttct ggcgaaatta actttgctat tctgtcaact tctgtatagc
    13861 tcattcttaa tgccctgcca acatctctca ccactgctct tgcgcccatg gtaccaaacg
    13921 ttgctatctg agcaacattt ttttcgccat atttctgtct gatgtaatct ataacctgat
    13981 cccttttggt atcgtcaaaa tcaaggtcaa tatcaggcat tgaaattcta tccggattca
    14041 aaaatcgttc aaataatagg tcatatttca aaggattgat ttcagtgatt ccgagaagat
    14101 acgcaacaag gctgccagga cctgaacccc gtcctgggcc aaccgctatc tttctttttt
    14161 ttgcctcgtt gacaatatcc tgaatgatca gaaagtaacc tgaaaaattc atttttttta
    14221 tgacatccag ttcgtatctg accctctttg taatctcatt atcctgatca atggtcatgc
    14281 caaattgtct ctgaagacca ttaaaaacaa gtttttcaag atattctgcg tctgacatac
    14341 cctctggaac aggaaaacga ggaatatgat gagttgaaaa atcaaaagtt acattgcact
    14401 tctcaaaaat ctcaacagtg ttttttgttg cttcaggtat ttccttaaaa agcaactgca
    14461 tttcttcagg actcttgaaa tagaattcag aacctgagaa tttcaacctg ttttcgtctt
    14521 ctacatttgt ccctgtttga atacaaagca ggatttcatg tgtaaaaaca tcgtttttcc
    14581 tgagatagtg acaatcatta gtagcaacaa tttttattcc attttttctg gcaatatcaa
    14641 taaccattct attcgctttt gtctgttcag ggataccatt ttccattaac tcaatataaa
    14701 aattttcttt tccgaaaaca tcaaggtacc agttcagtgc ggatatcgct ctgtcttggt
    14761 ttccagaaag aagcaattga ttaatctctc cctgaaggca accgcttaat acaatcaatc
    14821 catctgaaag ttttgaaagt aactctcgat caattctcgg tttatggtaa tatccttcaa
    14881 ggtaagaaag tgttgataac tctataagat tctcataacc cttgttgttg attgcaagaa
    14941 cagtcaggtg ataagcagat tctcgtttgt ccgtgctctt cttttcaaac ctgctaccag
    15001 aactcatata catctcgcat ccgatgatgg gctttatccc gtgattgagt gcactttcat
    15061 aaaagggaat aacgccaaaa agtccgccat ggtcagtaat tgcaacacct gacattccat
    15121 actggtgggc aagttttgta acctcatcaa tacggcacat accatcaagc agactatatt
    15181 cggtatgcag atggagatga acaaaatcct tcattttccg atcatcgcct gtttcttcgc
    15241 aacctggtat attttccctt ctgtcttaat agaaggcgct atggctattt cgacaagttg
    15301 cagttccctt atgttccttg caccaagact tgccattgaa agtcgcaggg caccaataag
    15361 attctgtgaa ccatcatctg tatttgatgg tccaaataat atttgtttta caggtcctgt
    15421 ggtaccaagt ctgattctag tgcctcttgg cagattttcg tcagatgttg ccatacccca
    15481 gtgatatccc tttgcaggtg cttcgtttgc tcttacaaac gcagaaccaa ccatcacagc
    15541 gtctgctccg cacgcaaacg ccttgcatat gtcaccactg gtaatcatac ctccatcagt
    15601 aataatgggc acatattttc ctgtgcgctt gtaaaaaaag tcccttgccg cggcacaatc
    15661 aacagtcgca gcaacctgtg gtacacctat tccaaggact tctcttgttg tacaggcagc
    15721 gcctggtccg ataccaacaa gcagtccgga aataccggtt tccatcaatt caagagcaac
    15781 ctgataggta acacaattac ccgcgcacac aggaattttc attgaagaac aaaactcaaa
    15841 aaagtcaagg ggtttataag tacttgaaat atgttttgcg gttgtcactg ttgactgcac
    15901 aacaaaaatg tcagccccag cactctgggc aatggcacca aatctttctg cgtgctgcgg
    15961 aatggaactg accgcgcata acactccccc atctttaatt tcctgaatcc ttttttcgat
    16021 aagatgttcc ttcaccggtt catgataaat cttctgaatc agtgcagtgg ctttttcctg
    16081 cggacaggca atgatttcct ctataacaga ataagggtcg tcgtaacggg tgtagatacc
    16141 cataagatta aaaacagcca gtcctccggt ttttccaaat gcgattgcaa atttcgggtc
    16201 aaccacacca tccatcgcgg cagccagaat tggaacttca agtcgtttcc cagcaataat
    16261 cacagaaata tcaatctcag cagggttaat cgtcaaactt ccaggtacaa gggaaatgtc
    16321 atcaaatcca taacaaacac gagccttccg accccttcct atccacatcc ccataaaatt
    16381 cctcctttca ccttgatgtt tctcttgcct gttttaaact cattaatagt tcaatattag
    16441 aatgggttga cttcaatctt gaaattagca gttccattgc ttcaacagga ttcaatggcg
    16501 caagaattt
//
LOCUS       DAORYU010000005        15351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_3107_length_15351_cov_4.310931, whole genome shotgun sequence.
ACCESSION   DAORYU010000005 DAORYU010000000
VERSION     DAORYU010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15351
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3107_length_15351_cov_4.310931"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="PLW07_00405"
     CDS             complement(<1..377)
                     /locus_tag="PLW07_00405"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOL49017.1"
                     /translation="MKDKRKTIIEAASAMIGEKGYSLTTIDEITKRAGIGKGTFYIYF
                     KNKDALFYAIIEENFTNFLNEVKTSIEKIDDFSEKIKKVIEMYLSHHEKNYFLFKILT
                     QEKPSLKKGAFIKFWNTFFSRWD"
     gene            complement(704..1783)
                     /locus_tag="PLW07_00410"
     CDS             complement(704..1783)
                     /locus_tag="PLW07_00410"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOL49018.1"
                     /translation="MEVNDFEFKKNFVETIERHKKLQFARKGCLISTFLPEEEHIANS
                     VKIPPLEEIDFRKDVLLLPKLLCDREEKLYRYRSDYPDDSIPVLALRYGSGIFAGMIL
                     GTLRFGANTSWIEPVGKTIEETINFPWKKDNEFIDIALSGFRYATKRMKNKCYVYLSG
                     YHSPLELAFILRGSEFFLDVYSCPDKVHTLIQQCDKALKWIYQLLEDNVRNENYGNIA
                     GFLWMERCLPFLSEDAAGLLSPQHYKEFGVPYTDEVFRRFGGGFLHVHTQSYHQMENI
                     SSMEHLTTYNWRPDPNTPEAISILEKLIDGARKKIVVIVADPQSIQQKVDLLSQGRFF
                     LLCYCKNRNEQNFIVDFVKEKLQIK"
     gene            complement(1770..3485)
                     /locus_tag="PLW07_00415"
     CDS             complement(1770..3485)
                     /locus_tag="PLW07_00415"
                     /inference="COORDINATES: protein motif:HMM:NF015177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HOL49019.1"
                     /translation="MKYPDVWGEGVIFAFSGIEGKTDWSHPFIATTGPDFGQFIFVFD
                     EKTQFGFKNLNRDKKIRASIVSSDIFLTENPATAMIFVEKDKIIGRYSSDFAPYLGNP
                     PQSRYANEPVISLENGNVALSILPDKKIFSCVFDLKSKESAIRKARKILSEVSVEKGI
                     KKKLDFFRTLDYPEFNSEIEEKTYLKAISIMKVNCESKQGKIKCLWTTPDRWPHKHMW
                     FWDSAFHTFGNKFISYQIAENTLLAVLGCQRNDGFISITMNPEKGRVYEHLTQPPLFA
                     WAALNLFRETRNYEFLNTVYNGICKYIRWLYTNRDRDGDGLLEWIVDDNPQSKCGECG
                     MDNSPRFDDISSDELLAAIDLNCFAINEMNCLSQIAKILKKMEESLVWKNIAEEKKKL
                     VDKFLWDDKDNFYYDRKQNGQFVRVKTPASLLPLLAEIPNIDKARKMHNTLIDKNIFW
                     TDFPFPSVAKNEKTFCKDMWRGGTWLNYNFLIYKGLMKYGFKKTATGLLLKTKNAVEK
                     WYGEKGSIFEFYDPDNKIPPRDLPRKDWLGTRGWTRTITDYQWSAAIYVAILHELKKD
                     KHGSE"
     gene            3609..5342
                     /locus_tag="PLW07_00420"
     CDS             3609..5342
                     /locus_tag="PLW07_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49020.1"
                     /translation="MVEIASVAAIVSTLSLGGYQLVKKGKDSVCINNLKQIGQALAMF
                     EADHDGFPEAVFYPKDTNDPRGVHNLLKKYGATSQLFFCPAVSQEFNRYGTNYLWNES
                     VKGKQSSDASNVWLMTEITSLYPELPTPHTGGYGVLYADGHAAIGKNITFPSFQKPSL
                     ASTESIEKPKEQPPVEEKKEEVQETKPLVRVDAYQIINLPPRIQAGKFVPITVKAVDN
                     GGNVVEKDEKVKILDLTQTIEPSEVVLQKGIGNVMIRVKKACENNVVIVRGPEGLWSS
                     SLEFAVESGQPASIEISPPTLVYAGIPATFIVTLKDAYGNTLTKEGIKILTSASNEAE
                     YSPEFFSDASGKIGASFIFHKAGENRVSFSIAGTTIRESCSVKVRSGLLERFEISPVK
                     SVVEAGTPVSITVKALDKYGNRVKGFIFDDNTFKPGYVQEDMSSGIWMETVTFEKAVS
                     ETCIVISDGMGHSGRSNSFSVIPSFPVQLQLLGFHPVAIQQQTFEVKFCVLDRYGNRI
                     SGLESSFVVEGITDYKISRIEDVYIFNAKFDDTGKKNIKISLKQENKNPLNLEFFIDI
                     LPKKTVLKKGE"
     gene            5344..5895
                     /gene="yfcE"
                     /locus_tag="PLW07_00425"
     CDS             5344..5895
                     /gene="yfcE"
                     /locus_tag="PLW07_00425"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006988.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HOL49021.1"
                     /translation="MNIVVISDTHGSLDAWNKIEKYISTETSCVLHAGDIYYSGPRNP
                     VPSGHAPGSLAERVNALNIPIICAKGNCDSDVDQLISKFPLCDPFAFVFHDGRKIVVT
                     HGHKYEKEKLVSLAKDWSVNIVVTGHTHLACLEKTDGIIFLNPGSCALPKNFPGIGLI
                     SNNSIELRNLIDGSVVERVSLIN"
     gene            5916..7190
                     /locus_tag="PLW07_00430"
     CDS             5916..7190
                     /locus_tag="PLW07_00430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HOL49022.1"
                     /translation="MAEIKYIKHVNLVCVGSELFYHVSNTNVHTATRILEMHDFCLNY
                     NVIVPDDLEKIIKSVKFCMDNGDAIIISGGLGPTFDDITREAVSELTGKNLSFSESAW
                     EDIKERFRTRGIENIPEKNRKQAMMLEGATKLTNTIGTAPGMMVEYKDRVIFLLPGVP
                     KEFEEMLQFQVIPELLRRKNDRVSKSFVRMGFGIVGEPEAVVEEKTDKLRKEIESQGG
                     IWTILALPYLIELWLKIPQSKKNVFCDVEEQLKEIFGVSFLGTGSSISIPESLFNLLK
                     EKNLTCCFAESCTGGLAGHLITEIPGSSEIFNGSIVAYSNKMKKKFLKVPKTILRKYG
                     AVSEQTAIAMAKGARKYGKSDVSLAITGIAGPSGGSVEKPVGLVYMAVALKNNHIISK
                     KFQFSGQRSVIKMRTALAGIDLLRRVIQETKL"
     gene            7187..8170
                     /gene="mltG"
                     /locus_tag="PLW07_00435"
     CDS             7187..8170
                     /gene="mltG"
                     /locus_tag="PLW07_00435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOL49023.1"
                     /translation="MRTVIIVGTVVAGFTIAIFAARFHASLYGEFQPPGTLVEIPKNL
                     MASDVAVLLKEKGIIKNTRLFLKVLNRNHTDRKLRSGIYEFSGKPYIYDVIQKLLKGE
                     IAYIKLVFPEGITCHEIGRILEQENICSSSEFDEFVKKESLEGYLFPDTYKFPYGVSK
                     EAVAKKMVERFWEIIQQIHPDATKLNQTELKKIVIIASIVEKEAEINSERPVIAGIFY
                     NRLRKNMPLQSCATIKYVIGNKKILTMQDIKTRSPYNTYLHRGLPPGPICNPGKESLK
                     AAINPAKTPYLFFVSKGDGTHYFSNTYSEHLSAKNYYITGDSFSDTEKKLP"
     gene            complement(8181..9158)
                     /gene="tsaD"
                     /locus_tag="PLW07_00440"
     CDS             complement(8181..9158)
                     /gene="tsaD"
                     /locus_tag="PLW07_00440"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOL49024.1"
                     /translation="MFILGIESSCDETSIGIVKDTQLIVNLIASQEEIHASFGGIVPE
                     LASRAHLEKIAHIFNIAIKRAGISPEQIDAIGVTHAPGLKGSLLIGVAFAEGLGYSLK
                     KPVYNVNHLYAHLAACYLDSSIQFPALGLVISGGHTSLFLLHSHTDLKLIGKTRDDAC
                     GEVFDKVGRMLNLPFPGGAYIEKLAQKGNEKKIKFPKAYIKDSLDFSFSGIKTAVYYY
                     ISKHGIENPSDIAASFQKTMVDSILAKINDALDLYPVKNFLFGGGVIRNKYMRTRIES
                     NLAERSVKSIIPEPSLCADNGGKIAIFTRYLILSGAKPQPYRISVVPTQ"
     gene            complement(9160..10035)
                     /locus_tag="PLW07_00445"
     CDS             complement(9160..10035)
                     /locus_tag="PLW07_00445"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HOL49025.1"
                     /translation="MKKKRITKGSGILILFLVFLILVTGLFLLEEIKKKSLPLQFHKQ
                     LPSEKHTKIKQEKLPVNLPEKTEGAGQIALILDDVGWNPDIINHVQKLQIPITLAVLP
                     DSPFGYKIAKQLANKPDVELLLHIPLEPENSNSETKIAPDFLTTSMGNEELKEKMDDC
                     LKKFEPYIVGVNHHMGSKFTANQEKMEVVLEEIKRKKLVYVDSLTSTQSVGYTLARQM
                     GIKTGKRDVFIDNSNEYDQISRSLEKAVLLAKEKGTAIAIGHARTTTLKVLESKIPEM
                     KDRGYKFVPITKVLK"
     gene            complement(10032..11213)
                     /locus_tag="PLW07_00450"
     CDS             complement(10032..11213)
                     /locus_tag="PLW07_00450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOL49026.1"
                     /translation="MKKKIIVWTAGFFLLSFLLGFNLELSQARKNEEELYRELERFVS
                     VVNIVKKEYVSPVDDKKLIYGALKGMLSSLDPYSAFLEPEASKELQMETSGEFEGVGM
                     EITLKDGIITVVSPIQDSPAWNAGIKPGDKIIEIDGQSTKGMNTWEAAQKLRGKKGTS
                     VTVSILREHATKIMKITLVRGVIKVNSVKSSILQDDIGYVRITNFQERTGSDLETVLK
                     ELQNQNVKALVLDLRNNPGGLLSSAVEVSELFLPEKKIIVSIQGRRPEDKKIYYSQKP
                     PLWNKPVVILINGGSASASEIVTGALRDNFPTFKTVGLKTFGKGSVQNLIKLDEDGTS
                     VKLTIAYYYTPSGTKIDGKGIGPDVKVEAPEDMEIGTPEKDIQLKKALEEVSKILTAN
                     K"
     gene            complement(11210..12658)
                     /gene="gatB"
                     /locus_tag="PLW07_00455"
     CDS             complement(11210..12658)
                     /gene="gatB"
                     /locus_tag="PLW07_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012446948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOL49027.1"
                     /translation="MNEFQTIIGLEVHVELLTESKMFCCCSTRFGKEPNTQVCPVCLG
                     LPGALPVVNEKAIELGIKTAAATNCKINEEFIFVRKSYFYPDLPKNFQISQYREPLAV
                     NGYLYVNGRKIGITRIHLEEDTGKLLHDEKTGNWSCIDFNRSGIPLMEIVTDPEISTP
                     EEAEDFLRQLRQIISYIGVSDCIMEEGSLRCDANISIKKTTDSQLGTKVEVKNMNSFR
                     SVRRALTFEQERMIEILEKGQNIVQETRLWNEQMQVTEGMRTKEEAHDYRYFPEPDLL
                     PFRIDAETIKKISLEIGELPDARKNRFQQNYGLSLYDSEVLTAEKPISDYFEKAVSCY
                     HKPKQIANLIQTELLGLLKENHVRITECRVKPEFLASIVKMIDNKEISSTVGKIVLRE
                     TFVSGTDPEIIVREKNLIQITDTEEIGSIIQEILNEHPQAINDYRAGKVQALGFLVGQ
                     VMKKSKNKANPGVVKQILEKKLTDRSNNTEEK"
     gene            complement(12660..13607)
                     /locus_tag="PLW07_00460"
     CDS             complement(12660..13607)
                     /locus_tag="PLW07_00460"
                     /inference="COORDINATES: protein motif:HMM:NF025839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4432 family protein"
                     /protein_id="HOL49028.1"
                     /translation="MENELLRIGILVDKGTDIVEFLYKPTDIDFMWRSPFPLYRQGFP
                     ETIHGKLGKFLDYYPGGWQECFPNAGRPCEYKGADIGLHGEVATLPWEFEVLENSERS
                     LKIRFWVRTLRTPFYLEKILTLKSGSSELTIEEKIVNEAGEDMDFLWGHHPAFGEPFL
                     DGNCIINIKKGARVEVMPGDGVSLTGLKQGIGSWPEVEGINGKPVDLGRVPGPDAKVS
                     DMIFISELKEGKYEIINPELSLGFRFEFPVDVFKYIWFWRVARGSFGYPWYGRTYSIA
                     LEPFSGRAILSDAVKNGYQLTLTPGKTLGVTLSVTAFRI"
     gene            complement(13674..15194)
                     /locus_tag="PLW07_00465"
     CDS             complement(13674..15194)
                     /locus_tag="PLW07_00465"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOL49029.1"
                     /translation="MRKSILFLPIVVFMAANVICANTIVQRHVIKKGETLSSVAQRYN
                     TTIEQLRKWNNLNSNRIYPGQTIIVNKYEAIQKPVDSHYYIVKKGDTLSSISKTTGIS
                     VEKLKAYNNMKTNAIMPGQKLVLVQQEKTQKSHTEIQKKDNISEANAYFVKKGDTLSS
                     ISKKTGISIDELKKLNNLKSSKLALGQKIILKTEEKLTDSDTQQVIAITESKTQQVAN
                     ENAPATPQATTISINKYHTVKKGETLSKIASKYRTTVASIKRLNGLKTNTIKPGKTLI
                     VARIVKEIPAPVINPTPIAPVSPKVYYRVKLGDTLESIAEKFGISVSALRETNLLTDG
                     KIKIGQTLVIPEMASYKEATEKETSLSSQEKIENTGSNLLASRIIENALNFINTPYRY
                     GGLSKTGIDCSGLAKKSYEAAGLSLPRTSREQAKTGEVIPVSALKPGDLIFFGYKGRI
                     NHVGIYIGDNKFIHASRESGKVVVANLEDSYYQKHLICARTLVNGEQAYWLEQESN"
BASE COUNT         4336 a         2900 c         2909 g         5206 t
ORIGIN      
        1 aagtcccatc ttgaaaaaaa agtgttccag aattttataa acgcgccttt ttttagagat
       61 ggtttttcct gggtcaaaat cttgaaaagg aaatagtttt tttcgtgatg cgaaagatac
      121 atttcgataa ccttttttat tttttcagaa aagtcgtcaa ttttttcaat actggttttc
      181 acttcattta aaaagttagt aaaattttct tcaataattg cataaaaaag ggcatcttta
      241 tttttgaagt aaatatagaa tgtgccttta ccaatccctg ctctctttgt aatttcatca
      301 atcgtcgtca gggaatagcc cttttcacct atcatggctg atgctgcttc aataatggtt
      361 ttccttttat ctttcatcct gcctcttttt ctgactgacc agtcagtcag agatgataac
      421 atgtctgtat ttacctgtca aggattttta cctttcatgg cgcgtagcaa aacattgttg
      481 taaatgtaaa gttgaaatat ggcttttccc ggcgaaggaa acaaattctg gaatttaagt
      541 gaattctcta ctgattaatc cataaaaacg tttttccgag ttgcaacggt tgcgttgaaa
      601 aaaggcacgc atatcatggg aattttctgt cgcctactcc tgattttaaa cggatagttg
      661 gcatatttta cttatataga atacgtattt gcggacgggc attttattta atctgcaatt
      721 tctcttttac aaaatcaacg ataaaattct gctcatttct atttttgcag tagcacaaca
      781 aaaaaaatct tccctgggat agaagatcaa ccttttgttg aatggattgc gggtctgcaa
      841 cgattactac gatttttttt cgtgctccat caatcagttt ttcaagaatg ctaatggctt
      901 ctggtgtatt cggatctggt ctccagttat aggttgtaag atgttccata ctggaaatgt
      961 tttccatctg atgatatgac tgagtgtgta cgtgaaggaa tcctccaccg aatctcctga
     1021 acacttcgtc ggtatatggt acgccaaatt ccttataatg ttgtggcgaa agaagtcctg
     1081 ctgcgtcttc acttaaaaaa ggaaggcatc tttccatcca aagaaagccg gcaatatttc
     1141 cataattttc atttctgacg ttgtcttcta atagttgata tatccatttt agtgctttat
     1201 cgcattgttg tatcaatgta tgaactttat cagggcacga gtacacatca agaaaaaact
     1261 cagaacccct caaaataaat gccagttcca gaggagaatg atagcccgat aaatatacat
     1321 aacacttatt tttcattctt tttgtagcat aacgaaaacc agaaagagca atatcaatga
     1381 attcattgtc tttcttccat gggaaattta tcgtttcttc tattgttttt cctacaggtt
     1441 ctatccatga tgtatttgcg ccaaacctca atgttccgag aatcataccc gcaaatatgc
     1501 cagaaccata tctcaaagca agcacgggta ttgaatcatc aggataatca cttctgtatc
     1561 tgtaaagttt ttcttcccta tcacagagta gttttggaag caggagaaca tcttttctga
     1621 aatcaatttc ttcaagtgga gggattttta ccgaatttgc aatgtgttcc tcttcaggca
     1681 gaaaagtgga gataaggcaa ccctttcttg cgaactggag tttcttgtgc ctttctatag
     1741 tttcaacaaa gttcttttta aattcaaaat cattcacttc catgtttatc ctttttcaat
     1801 tcatgcaaaa tagcaacata aatggctgca ctccattgat aatcagttat tgttcttgtc
     1861 catcctctgg ttccgagcca gtcctttcgc ggaaggtctc tgggtggaat cttgttgtca
     1921 gggtcataaa actcaaaaat tgaacccttt tcaccgtacc atttttcgac ggcgtttttt
     1981 gtttttaaca gtagtcctgt ggctgttttc ttgaacccat atttcatcag gcctttgtaa
     2041 atcagaaaat tgtagttgag ccatgttcca cctctccaca tgtctttaca aaaagttttc
     2101 tcattttttg caactgacgg gaaggggaaa tctgtccaga aaatattttt atcaataagt
     2161 gtgttgtgca tctttctggc tttgtctatg ttgggtattt ctgcaagtaa tggaagcaga
     2221 gaagctggcg ttttaactct tacgaactgt ccattttgtt tcctgtcata ataaaagtta
     2281 tctttatcgt cccatagaaa tttatcaacc agttttttct tttcttctgc aatgtttttc
     2341 catacaagag attcttccat ttttttcaag atttttgcta tttgagaaag acaattcatt
     2401 tcatttatgg caaaacaatt taagtcaata gccgcaagga gttcatctga agaaatatca
     2461 tcaaatcgtg gggagttgtc catgccacat tcgccgcact ttgattgtgg attatcatca
     2521 acaatccatt caagtagtcc atcaccgtct ctatctcgat ttgtgtacag ccacctgatg
     2581 tatttacaga ttccgttata cacagtgttc aaaaattcat agtttcttgt ttctctgaac
     2641 aaattcagag cagcccatgc aaaaagaggg ggctgggtaa ggtgttcata gaccctgccc
     2701 ttttcagggt tcatcgtgat ggagatgaac ccgtcatttc tctggcaccc gagtacagca
     2761 agcaaagtat tttcagcaat ctgataggaa ataaatttat ttccaaacgt atggaatgca
     2821 gaatcccaga accacatatg tttatgaggc cacctatcag gcgttgtcca gagacacttg
     2881 atttttccct gtttactttc gcaattcacc ttcatgatgg aaattgcttt gagataggtt
     2941 ttttcttcaa tctcgctatt gaattctgga taatcgagtg ttctaaaaaa atcaagtttc
     3001 ttttttattc ctttttcaac ggaaacctca cttaaaattt ttcttgcttt tctgatcgca
     3061 ctttccttgc tttttaaatc aaaaacgcat gaaaaaattt ttttatctgg taaaattgag
     3121 agagctacat ttccattttc aagagaaata acgggttcgt ttgcgtatct tgattgtggc
     3181 gggttcccaa gatatggtgc aaaatctgag gaatatcttc caataatttt atctttttct
     3241 acaaatatca tcgcagtggc agggttctca gtcagaaaaa tatcagaact tacaattgat
     3301 gcgcgaattt ttttgtctcg gttcagattt ttgaatccaa attgtgtttt ttcatcgaat
     3361 acaaaaataa attggccaaa atcaggacca gttgtggcaa taaatggatg cgaccagtca
     3421 gtttttcctt caattcctga aaatgcgaaa attactcctt caccccacac atcaggatat
     3481 ttcattgtta atccttgttt ttgaattttg ataaattata acaaattttc tgactcatgc
     3541 gaaaatttca ttttgaccga atgacaaaaa atttttataa tacatgtatg aagataaaag
     3601 gatttacatt ggtagaaata gcatctgttg ctgctatagt ttctaccctt tcactcggcg
     3661 gttatcaact tgtgaagaaa ggaaaggatt ctgtttgcat aaacaatctg aaacaaattg
     3721 gccaggcgct ggcaatgttc gaggcagatc acgatggttt tcctgaagca gtgttttatc
     3781 ctaaagatac taacgacccc cgcggggtac acaatttatt aaagaaatat ggtgccactt
     3841 ctcagttatt tttctgcccg gcagtaagtc aggagtttaa cagatacgga accaattatc
     3901 tctggaatga gtcggtcaaa ggcaaacaga gctcggatgc ttcaaatgta tggttaatga
     3961 ctgagatcac gtcactctat ccagaacttc ctacccctca tactggtggt tatggggttc
     4021 tttatgctga tggacacgct gcaattggaa aaaatattac ttttccttct tttcaaaaac
     4081 catctcttgc ttcaacagaa agcatagaaa aaccaaaaga acaaccgcct gttgaagaaa
     4141 agaaagaaga agttcaagaa acaaaacctc tggttcgtgt ggacgcatat caaattatca
     4201 atctacctcc aagaattcag gcgggtaaat ttgtgccaat tactgtaaaa gccgttgaca
     4261 atggcggaaa tgttgtggaa aaagatgaga aagtgaaaat actggatctc actcagacca
     4321 ttgaaccttc tgaagttgtt cttcaaaaag gtatcggtaa tgttatgatt cgggttaaaa
     4381 aggcctgtga gaataacgtt gttatagtac gtggacctga aggattgtgg tcaagttcat
     4441 tagaatttgc tgttgaatcg ggtcagcccg catcaattga gatttcaccc cccactcttg
     4501 tctatgcagg aattcctgca acctttatag ttacactgaa agatgcctat ggcaatacgt
     4561 taacaaaaga aggaataaaa atattaacct ctgcaagcaa cgaagctgaa tactctcctg
     4621 agtttttttc ggatgccagc ggaaaaattg gagcgtcttt cattttccat aaagccggag
     4681 aaaacagggt tagtttttct atcgctggta caaccatcag agaatcatgt tcggtgaaag
     4741 taagatctgg acttcttgag cggtttgaaa tcagtcctgt taaatcggtt gttgaagctg
     4801 gcacacctgt gtcgattaca gtgaaagccc ttgacaaata tggtaataga gtaaagggtt
     4861 ttatttttga tgataataca tttaaaccag gatatgttca ggaagacatg tcttcaggca
     4921 tatggatgga gacagtcacg ttcgaaaaag cggtttcaga aacatgcatt gttatcagcg
     4981 atggaatggg acattctggc aggtcaaaca gcttttctgt aattccatct tttcctgtgc
     5041 agttgcaatt gctagggttt catccagttg cgattcagca acagacattt gaagtgaaat
     5101 tttgtgttct tgatagatat gggaatcgta tcagtggtct tgaatcatct tttgttgttg
     5161 aagggatcac tgattataaa atctctcgaa ttgaagatgt gtacattttc aatgcaaagt
     5221 ttgatgatac aggcaaaaag aatataaaaa tttctttgaa acaggaaaat aaaaaccctt
     5281 tgaatcttga attttttatt gacattctac cgaaaaaaac ggttttgaaa aaaggggaat
     5341 agaatgaaca ttgttgtaat cagtgatact cacgggtctt tagatgcgtg gaataaaata
     5401 gaaaaatata tcagtacgga aacatcctgc gtgcttcatg ctggagacat atattattct
     5461 ggaccgagga atcctgtccc gtctggacac gcaccaggtt ctcttgctga acgcgttaat
     5521 gcattgaaca taccgatcat atgtgcaaaa gggaattgtg attctgatgt agatcaattg
     5581 ataagtaagt ttccgctatg tgacccattt gcattcgttt ttcatgatgg caggaaaatt
     5641 gtggttacgc acggacataa gtatgagaaa gaaaaacttg tttctctggc gaaagattgg
     5701 tctgtgaata tcgtggtgac cggtcataca catttagcat gtcttgaaaa aacggatggt
     5761 attatatttt tgaatcctgg aagttgtgcc cttccaaaga attttccagg tattggtttg
     5821 atttctaaca attccataga gcttcgtaac ctgattgacg gttcagttgt tgaaagggtc
     5881 agtctgatca actgaatttt ggtctcaata taacaatggc tgaaataaaa tacatcaagc
     5941 acgtaaatct tgtgtgtgtg ggaagcgaac tgttttatca cgtttccaat acgaatgttc
     6001 acacagcgac aaggattctt gaaatgcatg atttctgttt gaactacaat gttattgttc
     6061 ctgatgatct ggaaaagatt attaagtctg tgaaattttg tatggacaac ggagatgcaa
     6121 tcattatttc agggggactg ggaccgacgt ttgatgacat tacaagggaa gcggtctctg
     6181 aattgactgg caaaaatctt tctttttctg agagtgcctg ggaggacata aaagaaagat
     6241 ttcgaacaag gggaatagaa aatattcctg aaaaaaacag aaagcaggct atgatgctgg
     6301 aaggcgcaac aaaacttacc aatacaattg gaacagcacc agggatgatg gttgaatata
     6361 aagacagggt tatctttttg cttcctggcg ttccaaaaga atttgaagaa atgttgcaat
     6421 ttcaggttat tccagaatta ttaagaagaa aaaatgatag ggtttcaaaa tcttttgtta
     6481 gaatggggtt tggtattgtt ggtgaaccag aagcagttgt tgaagaaaaa acagataaat
     6541 tgagaaagga aatagaatca cagggtggta tctggacaat acttgctctt ccttatttaa
     6601 ttgaactctg gttaaaaatt ccccaatcaa aaaagaatgt attctgcgat gttgaagaac
     6661 aattgaaaga gatttttggg gtttcttttc tgggtacagg tagtagtatt tcaattccag
     6721 aaagtttgtt caatctttta aaagaaaaaa atctgacgtg ttgttttgct gaatcatgta
     6781 cgggtggtct tgccggacat ttgattactg agatacctgg aagttcagag attttcaatg
     6841 gttctatcgt tgcatattca aacaaaatga aaaagaaatt tctgaaggta ccaaagacga
     6901 ttttgcgaaa atatggcgct gtgagcgaac aaactgcgat tgctatggca aaaggggcga
     6961 gaaaatatgg aaaatccgat gtttctcttg ccataactgg aattgcagga ccttcaggag
     7021 gcagcgtaga aaaaccggtt ggacttgtat atatggcggt tgcattgaaa aacaatcata
     7081 tcatatccaa aaaatttcag ttttcaggtc agcgctctgt aataaagatg aggacagcgc
     7141 tcgcaggtat tgatttgtta cgccgcgtga tacaggagac gaagttatga gaactgtgat
     7201 tattgttgga actgttgttg ctggtttcac aatcgccatt tttgctgcca ggtttcacgc
     7261 aagtttatat ggggagtttc agcctcctgg aacacttgtt gaaataccaa agaatttgat
     7321 ggcttctgat gttgctgttc tattaaaaga aaaaggtatc ataaaaaaca cacgactttt
     7381 cttaaaggtt ctcaacagaa atcatactga tagaaaactg cgtagcggta tttatgagtt
     7441 tagtgggaaa ccgtatatct acgatgtcat ccaaaaactt ttgaagggag aaattgctta
     7501 cataaaactt gttttcccgg aaggtattac gtgtcatgaa ataggcagaa ttcttgaaca
     7561 ggagaacatc tgttcttctt ctgaattcga tgaatttgta aaaaaggaat ctcttgaagg
     7621 atatttattc cctgatacct ataaatttcc atatggtgtt tcaaaagaag cagttgcaaa
     7681 aaaaatggtt gaaagattct gggagattat tcaacagatt catccagatg ctacaaaact
     7741 taatcaaaca gaacttaaga aaattgtgat tattgcatcc attgtggaaa aagaggctga
     7801 aataaattca gaaaggccag tcattgccgg gattttttac aataggttga ggaaaaatat
     7861 gccactgcaa agttgtgcca cgattaagta tgtgatcggt aacaaaaaaa ttctcaccat
     7921 gcaggatata aaaaccaggt caccctataa tacctatttg cacagaggac ttccgccggg
     7981 tcctatatgt aatccaggaa aggaatcatt gaaagcagca atcaatccgg caaaaacacc
     8041 atatctgttt ttcgtttcaa aaggtgatgg tactcattat ttttcaaaca cttattctga
     8101 acacctgagc gccaaaaatt attatattac aggtgattct ttttctgata ctgagaaaaa
     8161 acttccataa ttcagtattc ttactgagtc ggcacaacag aaattctgta tggctggggt
     8221 tttgctcccg agagaatcaa atacctggta aatatggcaa tttttccccc attatctgcg
     8281 caaagggatg gttctggaat aatggatttg acacttcgtt cagcaagatt ggattcaatt
     8341 cttgtccgca tatatttatt gcgaatgacg ccacctccaa ataagaaatt ttttaccggg
     8401 tagaggtcaa gagcatcgtt gatttttgcc agtattgaat cgaccatggt cttttgaaaa
     8461 cttgcagcaa tatctgaggg gttttcaatg ccgtgttttg aaatgtagta ataaaccgct
     8521 gtttttattc cactgaaact gaaatcgagt gaatctttga tgtatgcttt tggaaattta
     8581 attttcttct catttccttt ttgagcaagt ttttctatgt atgcgccacc aggaaaaggt
     8641 aagtttagca ttcttccaac cttgtcgaat acttcaccac aggcatcatc tcttgtttta
     8701 ccgataagtt ttaaatctgt gtggctatga agtaagaaca aacttgtatg accgcctgag
     8761 atgacaaggc ccagggctgg aaattgaatg gatgaatcaa gatagcatgc tgcaagatgg
     8821 gcatacaggt gattgacatt gtatactggc tttttcaaac tgtacccgag tccttctgca
     8881 aatgctactc caatcagtaa acttcctttc agtccaggtg catgtgtgac accaatagcg
     8941 tctatttgct ctggtgaaat cccagccctt tttattgcaa tgttgaagat atgtgcaatt
     9001 ttttcaagat gcgcgcggct tgcaagttca ggaacaattc ccccaaacga ggcgtgaatt
     9061 tcctcctggc ttgcgatgag atttacaatt aattgggtgt cttttacgat tccaatggat
     9121 gtttcatcgc aggagctttc aataccaaga ataaacatgt tatttgagga cttttgtgat
     9181 gggaacaaac ttgtatcccc ggtctttcat ttcaggaatt ttgctttcaa gaaccttcag
     9241 ggtcgttgtt cttgcgtgtc caatagcaat ggctgtacct ttttctttag caagtagaac
     9301 tgctttttca aggctcctgg atatctggtc gtattcattt gaattgtcga tgaaaacgtc
     9361 tcttttgccg gtttttatac ccatctgtct ggcaagggtg tatccaactg attgtgttga
     9421 agttaaactg tccacataga cgagtttctt tcgtttaatc tcttcaagca cgacctccat
     9481 tttttcctgg tttgctgtaa attttgaacc catatgatgg ttgactccaa caatgtatgg
     9541 ttcaaatttt tttaaacaat catccatttt ttcctttaac tcttcgtttc ccatgcttgt
     9601 tgtcagaaaa tccggagcaa ttttggtttc agagttggaa ttttctggtt cgagaggtat
     9661 gtgtagaagc agttcaacat ctggtttatt tgccagttgt tttgctattt tataaccaaa
     9721 tgggctgtcg ggtaatactg ccagggttat gggtatttga agtttttgca cgtgattgat
     9781 aatgtcagga ttccatccaa catcatcaag tattagggca atctggcctg ctccctcggt
     9841 tttttcaggc agattcacag gaagtttttc ctgtttaatt tttgtgtgtt tttcagatgg
     9901 taactgttta tgaaattgaa gaggtaatga tttctttttt atttcttcca gcaaaaaaag
     9961 tccagtcacc agaatcagaa acacaaggaa aaggattaaa atcccagacc cttttgtaat
    10021 acgttttttc ttcatttatt tgctgtaagt atttttgata cctcttcgag ggcttttttc
    10081 agttgaatat ctttttccgg ggtaccgatt tccatatctt caggcgcttc gacttttacg
    10141 tcaggtccta ttccttttcc gtctattttt gttcccgacg gtgtatagta gtatgcgatt
    10201 gtcaatttta cagaagtacc gtcttcatca agttttataa ggttttgcac agagcccttt
    10261 ccgaatgttt ttaatccgac tgtcttaaac gtagggaaat tgtctctcaa agcaccggtt
    10321 acaatttcag aagcactggc actgccacca ttgataagaa tgacaactgg tttattccac
    10381 aatgggggtt tttgcgaata atagattttt ttgtcttctg gtcgtctccc ctgtatggag
    10441 acaatgattt tcttctccgg taaaaatagt tcacttactt ctactgccga acttaataaa
    10501 ccaccggggt tgtttcttag atcaagtacc aatgctttga cattttgatt ctgtagttct
    10561 ttaagcactg tttccaggtc actaccggtt cgttcctgaa aatttgtaat tcttacataa
    10621 ccgatatcat cctgtaaaat agaacttttt acagagttaa ccttgatgac gcccctgacc
    10681 agggtaattt tcataatttt tgttgcgtgt tcacgtaaaa tacttactgt taccgatgtt
    10741 ccttttttcc ctctcagttt ttgtgctgct tcccacgtat tcattccttt tgttgattga
    10801 ccgtctattt caatgatttt atctcctggt ttgatcccgg cattccaggc aggcgaatcc
    10861 tgaattggac tgaccactgt gataataccg tctttcagag tgatttccat tccaacgcct
    10921 tcaaactcac ctgatgtttc catttgcaat tcttttgatg cttcaggttc aaggaaagcg
    10981 ctgtaagggt cgagagatga gagcattcct ttgagcgcgc catagataag ttttttgtca
    11041 tcaacaggtg atacatattc cttttttaca atattaacaa cactgacgaa ccgttcaagt
    11101 tctctgtaaa gttcttcttc gttttttctt gcctgggaaa gttcaagatt gaatccgagt
    11161 aaaaagctca acagaaaaaa acctgctgtc cagactatta ttttcttttt catttttcct
    11221 ccgtattatt actcctatcg gttaattttt tttcaaggat ttgttttaca actcctggat
    11281 ttgctttatt ttttgatttt ttcatgacct gcccaaccag aaatccaagt gcctgtactt
    11341 ttcctgccct gtagtcattg attgcctgcg gatgttcatt taatatttct tgaatgatac
    11401 tcccaatttc ttctgtatca gttatttgaa ttaagttctt ttctctgaca atgatttcag
    11461 gatctgttcc acttacaaat gtttctctga gaacaatttt cccaacagtg ctgctgattt
    11521 ctttattatc aatcatttta acaatgcttg caagaaattc gggtttgact ctgcactctg
    11581 ttattctaac atgattttct ttcaacaaac caagaagttc tgtctgaatc aggtttgcta
    11641 tttgtttcgg cttatgataa caggaaacgg ctttttcaaa atagtctgat atgggttttt
    11701 cagctgtcag aacttcagaa tcatagagcg acaaaccata attctgttga aatctatttt
    11761 tcctggcatc gggcaactcg cctatttcaa gcgaaatttt ctttattgtt tcagcatcta
    11821 ttctgaaagg taataaatcg ggttcaggaa aatatctgta gtcatgggct tcttcttttg
    11881 ttctcatacc ctctgttacc tgcatctgtt cattccacaa tcgagtttcc tgaactatat
    11941 tttgtccttt ttctaaaatt tcaatcattc tttcctgttc aaaagtcagg gcgcgcctca
    12001 cagacctgaa ggaattcata ttttttacct caacctttgt gccaagttgt gaatctgttg
    12061 tttttttaat tgaaatattt gcgtcgcatc ttaatgagcc ttcttccatg atgcagtcgc
    12121 ttaccccgat atacgagata atctgcctga gctgacgaag aaaatcttct gcttcctccg
    12181 gggttgaaat ttcaggatca gtcactattt ccattagagg aataccggat ctgttaaaat
    12241 ctatgcacga ccagtttccc gttttttcat catgcaacag tttacctgtg tcttcctcaa
    12301 gatgaatgcg agtaatacca atttttctgc cattaacgta cagatatccg tttaccgcga
    12361 gtggttcgcg atactgtgat atctgaaagt tttttggtaa atctggatag aagtaagatt
    12421 ttcttacaaa aatgaattcc tcattgattt tacaatttgt ggcggcagca gtttttatac
    12481 caagttctat tgctttttca tttaccacag gaagcgctcc tggcagtccg agacacacag
    12541 gacacacctg tgtgttcggt tcttttccaa atctggtgga acagcagcaa aacatttttg
    12601 attccgtcaa aagttcaaca tgcacttcca atccaataat tgtttgaaat tcattcattt
    12661 tagattcgga aggctgtaac tgaaagtgtt acgccgagag ttttaccagg agtcagggtt
    12721 aattgataac cattttttac tgcatcagac agaattgccc ttccggaaaa aggttccagt
    12781 gcgatactat aggttcgtcc ataccatgga tatccaaaac tgccacgcgc aaccctccag
    12841 aaccatatgt atttgaaaac atccacggga aattcgaacc tgaaccccag agacaattca
    12901 ggattgatga tttcgtattt cccttctttt aattcagaaa taaatatcat atctgatact
    12961 tttgcatcag gtccaggaac cctgccaagg tcaactggtt ttccattaat gccttcaacc
    13021 tcgggccatg agccaattcc ttgcttaagg cctgtcagag aaaccccatc tcctggcatg
    13081 acttcaaccc ttgcaccctt tttaatgttg atgatacagt ttccatcaag gaacggttct
    13141 ccaaatgcag gatgatgtcc ccatagaaag tccatgtctt ctcctgcttc attaactatt
    13201 ttttcctcga tggtaagttc agatgaaccg gattttaatg tgaggatttt ttcaagatag
    13261 aatggagttc gcagtgttct cacccagaat ctgattttca atgacctttc tgaattttcg
    13321 agtacctcaa attcccatgg aagggttgct acttcaccat gcaggccgat gtcagcgcct
    13381 ttatattcgc aaggtctacc ggcatttgga aagcactcct gccatccacc gggataatag
    13441 tcaaggaatt ttccaagttt tccatgaatt gtttcaggaa atccctgtct gtacaatgga
    13501 aacggacttc tccacatgaa atcaatatca gttggtttat aaagaaattc cacaatatcg
    13561 gttcctttat ccaccagaat acctattcgt aacagttcat tctccatata caaaattttc
    13621 ccgagtttcc attcataaca agcccatcgg cagccttttt tattagaaaa catttaattt
    13681 gactcctgtt ccagccagta agcctgttcg ccattgacta atgttctggc gcatatcaaa
    13741 tgtttttgat agtaagagtc ttcaagattg gcaaccacaa cctttcctga ttctctgctt
    13801 gcatgtataa atttattgtc tcctatgtaa ataccaacgt ggttaattct tcctttgtaa
    13861 ccaaagaaaa tcaaatcacc gggtttcagc gcacttacag ggattacttc acctgttttt
    13921 gcctgttccc ttgatgttct cggaagagaa agtcctgctg cttcatagga ctttttcgcg
    13981 aggccagagc aatctattcc tgttttggat aatccaccat agcgatacgg tgtattgata
    14041 aaattcaggg cattttcaat gattcttgaa gcaagaaggt tagaaccggt attctctatt
    14101 ttctcctggc ttgacagaga ggtttccttt tctgttgctt ctttataact tgccatttca
    14161 ggtattacca gtgtttgacc gatttttatt tttccatcag ttaatagatt tgtttccctg
    14221 agcgctgata cgctgatacc aaatttttca gctattgatt caagggtatc gccaagtttt
    14281 accctataat aaacctttgg agaaaccgga gcaataggag tgggattaat cacaggggca
    14341 gggatttctt tcactattct ggcaacgatg agcgttttac cgggtttgat tgtatttgtt
    14401 ttcaatccat taagacgttt tatactggca acagtagtgc gatatttgct cgctatctta
    14461 ctcagtgttt ctcccttttt cacggtatga tatttattga tggatattgt tgttgcctga
    14521 ggagtagcgg gcgcattttc gtttgcaacc tgctgggttt tgctttctgt tattgcgatt
    14581 acctgttgtg tatcagaatc tgttagtttt tcttctgttt tcaaaatgat tttctgaccc
    14641 aatgcaagtt ttgaactttt taagttgttc aattttttca attcgtcaat tgaaattcct
    14701 gtttttttgc tgatagaaga aagtgtatct ccttttttca caaagtacgc attcgcttca
    14761 gagatgttgt cttttttctg aatttctgta tgagattttt gtgtcttttc ctgttgaaca
    14821 agcaccagtt tctgcccggg cataatggca tttgtcttca tgttattgta cgcctttaat
    14881 ttttcaactg aaattcctgt tgttttgctt atggatgaaa gtgtgtctcc cttttttaca
    14941 atgtaataat gagaatcaac aggcttttgt atcgcttcgt atttgttaac gataatagtt
    15001 tgacctggat atattctgtt tgaatttaga ttattccact ttcttaattg ttcgatggtt
    15061 gtattatatc tttgtgcaac tgatgatagt gtttcgccct tcttaatgac atgacgttga
    15121 acaattgtat tagcacaaat tacgtttgca gccataaata caacgattgg taaaaaaagt
    15181 atggattttc tcacctttcc cccttttcaa gaggttcaaa catttttgca atttcttttt
    15241 ttttctcttc aaaatacgta tcaatttttg attttaatcc ttccattcct tcaagaattg
    15301 aaattctctc tgccaatttt tcctgttgtt caataataat ggaaagtgct t
//
LOCUS       DAORYU010000006        14708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_3254_length_14708_cov_4.947246, whole genome shotgun sequence.
ACCESSION   DAORYU010000006 DAORYU010000000
VERSION     DAORYU010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14708
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3254_length_14708_cov_4.947246"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="PLW07_00470"
     CDS             complement(<1..269)
                     /locus_tag="PLW07_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49030.1"
                     /translation="MSIEICKKILNRQFTERVGRTEYCDHPRLIEHIAGIYQKAHPDD
                     TRNPYKKFLDWVPYDLIWNANDGPVGWSELGRTTDMGHAVYAEGG"
     gene            complement(277..2394)
                     /locus_tag="PLW07_00475"
     CDS             complement(277..2394)
                     /locus_tag="PLW07_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOL49031.1"
                     /translation="MDERIKKILGKMSIEEKVAQLTCIERNELLEKEHFSKEKAIARL
                     KNGAGQVSPILRPFGPEEGTRISNEIQKIALEESSSKISVIIHDECIHGCMAKKSTSF
                     PQSIALASSWDPDMMYQVAKIIGKETRSRGIHQALSPTINIARDARHGRVEETYGEDP
                     YLTSKMAVAFIKGLQSEKVIATPKHFAADFAGDGGRDSSAVFFSERILREVFFPAFKA
                     SIMEAKAWSLMAAYNSINGIPSSSNRWLLTDVLRNQWGFEGFVVSDYGSVWGLYGSHR
                     VASTVAEAGKLALEAGLDIEIPASSCFSHLARLVKEGRVSEATVDRVVERVLKGKIWL
                     GLFENPYADPAQASLICDCDQHRQFALKVAEQCMVLLENNGILPLSKDIGSIAVIGPN
                     ADAIRLGGYSGSGIKVVSVFEGIKNSVSKRTRVYFAEGCRVNDKDKSGFSKAVNAAAK
                     ADVAILVMGNSAETEGENRDRCNLNLPGVQEDLIRAIADTGTPVIVVLINGSAVTMVN
                     WFDSVDAVLEAWYPGEEGGNAVANIIFGNSNPAGKLPITFPRFTGQLPLYYNHLPCLR
                     KYDYVEVRGFQPMFPFGYGLSYTQFEYRNLKVSPEKFSGKGNVNVSVEVENTGNYEGD
                     EIVQLYLRDVVSSIIRPVKELKRFSKVHLKPGEKTKVEFALEESDLSFLDENFKSVLE
                     PGVFEVMVGKNSTDLLITKFELK"
     gene            complement(2640..3188)
                     /locus_tag="PLW07_00480"
     CDS             complement(2640..3188)
                     /locus_tag="PLW07_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49032.1"
                     /translation="MNRDGKEEIIVAYYTSPHDYWIEKDGKKVADERFYDRARVAIFE
                     IKKMWESKGYGNLFDINFLPTTLKKEKIEYPWYVFGAKDIDGDGFLELIFSRTEYTAM
                     RGVIEIWRWNGKEFTQKLITDGELLFQTTEKDLEVVSISYYAGEITEIHYTYDKSLGK
                     YKIKSQFTKVLSDYMRENTKGK"
     gene            complement(3345..4271)
                     /locus_tag="PLW07_00485"
     CDS             complement(3345..4271)
                     /locus_tag="PLW07_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49033.1"
                     /translation="MLKLILFFSSILLMASFNFHFLSLFRENLCWCEDSNITDVEKNI
                     KQILTEEEWNRYLELKEIDAKRKEISIKYWQSVGPSFDVKNPKDKEQAERYKEELDMY
                     DKIWEKKAKEFWEILAPQQEKAYLLGVEIDKIEPESSAWIFASIFSDIKTNSDTLLKS
                     YLYFHKYQNKMRESLKGRMIRGIFSYLENINFHQEKDAINEALKFLEESKKPQEEKVI
                     VMQFLWDVWSKFFPRIEKMENKKEIYGEMNKQVLNIVEKLKNYLEKPELSAKSKEILE
                     KFKDYYLKQDLPEDFKNTEGYTDVIKYVNRIN"
     gene            complement(4265..4960)
                     /locus_tag="PLW07_00490"
     CDS             complement(4265..4960)
                     /locus_tag="PLW07_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49034.1"
                     /translation="MLTFIVLFLTAGFSYVISWWFIERDRRIIKEKGIIIFVISLGLF
                     GLDYSLTIMPMIFESHSHIINLTKVFFWKHFWFFILSVIFLKHVLIQEKNFGYYFFIP
                     VFFSVLLVSFFETIILLDSDELNEYFISDFVGWVSYFGVFGGIVSGLLFGTLKTVQKI
                     SLSLKKILTLLFYILFLGLCSLLPAYAVSFFIPKESFFHLFIVPVFVYVISFIIFGEI
                     KNFMNRRGGGKKC"
     gene            5768..6139
                     /locus_tag="PLW07_00495"
     CDS             5768..6139
                     /locus_tag="PLW07_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49035.1"
                     /translation="MQWKPLGKGLFAGTKTVEIRSICIPDEDGNLKRRRTTSCWNPAN
                     PKFSKTPAIGRLIKDESGKIGILVSGKHGTHIKIGKFASAIPFVFIAINCIAKKPRAM
                     LLKDLPVELYSEGNLIFAREK"
     gene            6185..6577
                     /locus_tag="PLW07_00500"
     CDS             6185..6577
                     /locus_tag="PLW07_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013100294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II SORL domain-containing protein"
                     /protein_id="HOL49036.1"
                     /translation="MEEKKVYKCESDILCGVNRPKDTANMTDFEKKHVPVITAPDKVK
                     RDEEFEVTVEVGKLVPHPNEPGHFIQWVELYSGDTFLARAYFNGGSASPKVTFRVKLS
                     HSHGFLKAWEMCNLHGLWQGEKPIEIVD"
     gene            6588..7211
                     /gene="wrbA"
                     /locus_tag="PLW07_00505"
     CDS             6588..7211
                     /gene="wrbA"
                     /locus_tag="PLW07_00505"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002683745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HOL49037.1"
                     /translation="MATKILVLYYSMYGNVFQMAQEVVKGIQQVQDAHPLLRKVPELL
                     PESIIQGNSDIKKAQELQKDVPVATLDDLKECDGLLIGTPTRFGNMCSQMRNFLDKTG
                     PLWLKGALIGKPAGIFTSSVSLHGGQETTCLATMLTLLHHGMVIVGIPSSNPDLMKTN
                     SGGTPYGASHVVGMEENFRPLSEEEKKLCNFLGKRVAEIAKKLKTNC"
     gene            7226..7729
                     /locus_tag="PLW07_00510"
     CDS             7226..7729
                     /locus_tag="PLW07_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOL49038.1"
                     /translation="MKKMTEANLKAAFAGESQAHMKYLIFAKKAEEEGKKNVARLFEA
                     IALAEQVHATNHLKALGEVGDTVENLQTAISGETYEVEEMYPAFDAVAKLQEEKAAQK
                     SINYAISAEKVHAGLYVKAKQAVESGTDIEIGKISICKMCGWTIEGDAPDRCPLCNAG
                     KEYFKTF"
     gene            7756..7905
                     /locus_tag="PLW07_00515"
     CDS             7756..7905
                     /locus_tag="PLW07_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49039.1"
                     /translation="MCGCCCCGSRKKEKKEYECAVNTDKCPKKTVDEGEPVPVCCGKP
                     MKPAK"
     gene            7994..8722
                     /locus_tag="PLW07_00520"
     CDS             7994..8722
                     /locus_tag="PLW07_00520"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOL49040.1"
                     /translation="MLRSPENLSKRPVLLITIAADCHHTLNHPDYNIISNVFLAAGHR
                     VIGFDLPNHGEHIDNYGEGLGGWANAISDGVDIFEKIRKIGSSIIDFAIKEKLAWENT
                     IVMTGVSRGGLSAMHIMAYDERVYAAVVHAPCTDLGKIREFKSLKDNPVIASSNVINL
                     VNRLANRFLLITIGEKDPRVDEKSCLEFYARLFATSDTIKPSIFILEGETHGNTAFND
                     AAYIAGASFLLEKISLRIKRKVND"
     gene            complement(8712..9701)
                     /gene="rfaD"
                     /locus_tag="PLW07_00525"
     CDS             complement(8712..9701)
                     /gene="rfaD"
                     /locus_tag="PLW07_00525"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HOL49041.1"
                     /translation="MKKYHIVTGGMGFIGSALVWQLNEMGIDDIIVVDIPGQSESWKN
                     LVNLKFADIFDRDEFILKLEKGTFNGCISGILHMGACSDTTEKDFSFLLENNYHYTKK
                     LAIWANARNCRFIYASSAATYGNGPEFSDNEEKLHLLKPLNRYGYSKHLFDLWAYKNQ
                     LLSKIAGLKFFNVFGPNEYHKGEMKSVVVKKYFEILKTGTAQLFKSYRKDSPDGEQKR
                     DFIYVKDAVKMTLFIYFHSAINGIFNIGTGKARSFNDIVKIAFKTLGKRENIQYIEMP
                     ESIKNSYQYFTQADISKLRAAGYQENLFTLEQAIEDYIKNYLQHQDPYLKVNR"
     gene            complement(9707..10105)
                     /locus_tag="PLW07_00530"
     CDS             complement(9707..10105)
                     /locus_tag="PLW07_00530"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOL49042.1"
                     /translation="MQTKKASTRSPKVLSSADKKLAVGIDILERARINIALRRHGQRF
                     LKKIYTQRERKSFPEEKEIYFSIGFSMKETFWKTLPENIQKKTYFNDIEILWDGDNLE
                     LFHSGKKVRNVHYSYAFNEKYVLTTVVRGI"
     gene            complement(10050..10589)
                     /gene="dcd"
                     /locus_tag="PLW07_00535"
     CDS             complement(10050..10589)
                     /gene="dcd"
                     /locus_tag="PLW07_00535"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HOL49043.1"
                     /translation="MVLPDKWIKRNAKKGLIEPFFESSVKKGVMSYGVSSYGYDFMLA
                     DEFKVFTGSNTIDPKNPDPGSFKNFTGKVCEIPPGSFVLGRSVEYFRIPRNVLGICQG
                     KSSYARSGIVVYVTPLEPEWEGHITIMIANLTPVPGRVYAGEGICQVIFLKATGECEK
                     SYADKKGKYQKSKGIIFGR"
     gene            complement(10593..10919)
                     /locus_tag="PLW07_00540"
     CDS             complement(10593..10919)
                     /locus_tag="PLW07_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49044.1"
                     /translation="MKKIISVLFIGFAIVFTGCAIHPGFRAEREGRSMITDYPMNKVA
                     VVRAIDQHRVIYGMTMEEVIASWGEPELQHELIINGKVYQSWAYRKNNRTHIIYFHHG
                     IVVDIR"
     gene            complement(10962..11816)
                     /gene="larB"
                     /locus_tag="PLW07_00545"
     CDS             complement(10962..11816)
                     /gene="larB"
                     /locus_tag="PLW07_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HOL49045.1"
                     /translation="MVNRIYNYFSFGHGIILTKLKKISINLRTQADTVKYNIMEKEEL
                     YQILRKFKNGHYSFDDVFEQLKILPFSDLRHSKIDHHRTLRVGFPEVVYGEGKSVDHL
                     VSIIRELSGKYKNFIITRISREKVDEILKTYPELVYHESARILTGKPINKQRKKEVAV
                     LCAGTSDYFVAEETAVTLEILGKGVERIYDVGVSGIHRIVPHLKKLNQCKILIVIAGM
                     EGALPGVIAGMTGKPVIGVPTSVGYGTNLKGFAPLLTMLNSCAPIVVVNIDNGFGAAF
                     FAWLVLRK"
     gene            11890..12024
                     /locus_tag="PLW07_00550"
     CDS             11890..12024
                     /locus_tag="PLW07_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49046.1"
                     /translation="MTLNLSARLVIISVSDYMVAPAHSIPYGAKPENIAAMIEVLQHQ
                     "
     gene            12035..13090
                     /locus_tag="PLW07_00555"
     CDS             12035..13090
                     /locus_tag="PLW07_00555"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOL49047.1"
                     /translation="MNGKQRSLAVLNHQNFDRYPLFDILTNDAAIEYYAGKKLTVENG
                     KQVVHAALDNMVDATRWIIMYPQKEEKIIAEDGTIIEQKRWTAWYHRRFTLNLDDVVK
                     NIKNEIEYFSNPENIEKEAIALDARIEQLVQIRDEFNNIFLFGNYLIKTGIQLYAGIG
                     LELFSYLITDYPDIFDQWMNLGTEFKVQVINRAKNVSEFPAIFDCEDLASKNGPLFSP
                     EIMKKYFYPHLEKLVDVYHKKNVKFIFHTDGNVMPILDNLVATGIDGLNPLEVIAGMN
                     LKEIRKRHKDLIMLGGIDCSQLLPYGTREQVRQATIQAIQDGGPWYFPGSSTELHDNI
                     PLDNIRVMVDTIRETRL"
     gene            13132..>14708
                     /locus_tag="PLW07_00560"
     CDS             13132..>14708
                     /locus_tag="PLW07_00560"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOL49048.1"
                     /translation="MKKYYFIMVLLVCLCGCSVSYYEKSADREVSSILREKQQPAEKG
                     KIKDSGLVDETRHETIFLNLKDALILAAKSNREYQTAKEDVYLSALSLTYQRYLYGVQ
                     RDASGSVEWNSASGQDIVSGNLNLSLVKWLAQGAQITFDIGQYLTKYLTGSKENIYQT
                     ALSLNIFQPLFQGAGRLVAQENLVQAERNVVYQIRSFLRYQRSFSVQVAQKYFNIILA
                     QNNLDNYWKNYVFLKDTRERVEMLAAAGRIPPLQADQARQNEYRAYQQWIQAGNNYKQ
                     QEDEFKIFLGLSPETVIVLDKKALELFLENGLNPIDINTETAIEYALSNRLDLMTYSD
                     YVGDAERNLAIAKNLLRSRIDFSGTVSSSTEEKSTFDLELKQPSYSAGIDFELPTGKI
                     SDRNRYKQALINFDRKKRDFTLKRENIKLEILNSIRDLEEAYQSYIVQKNSLQLATRR
                     VESTDLMLQAGRATTRDLLEAQESFLSAKNSLSSAIVNYLLSYLEFLKNTEQLQLDSD
                     GVWKGDLYEKIIGENPE"
BASE COUNT         4690 a         2880 c         2559 g         4579 t
ORIGIN      
        1 atgcctccct ctgcataaac agcatgtccc atgtctgttg ttcttccaag ttcagaccaa
       61 cctacaggtc catcatttgc gttccatatc aaatcataag gaacccaatc aagaaatttt
      121 ttatagggat ttcttgtgtc atcgggatgg gctttctggt aaataccagc aatgtgttca
      181 atcaatcgag gatggtcaca gtattctgtt ctaccaaccc tttcagtaaa ctggcggttc
      241 aaaattttct tacagatttc tattgacata gcgagtttat tttaattcaa atttcgttat
      301 aagcaaatca gtactatttt tacctaccat tacctcaaac actcctggtt caagtactga
      361 tttgaaattc tcatcaagga aggataaatc gctttcctcc agagcaaatt ccacttttgt
      421 tttttctcct ggcttcaggt gaacttttga aaatcttttt aattccttta caggcctgat
      481 tattgaactg acgacatccc tgaggtatag ttgcacaatt tcgtcaccct cgtaattacc
      541 tgtgttttcc acctcaaccg acacattgac attccctttg cctgaaaact tttcaggtga
      601 gacctttaaa ttcctgtact caaattgagt ataactgagt ccatagccaa aaggaaacat
      661 gggctgaaat ccccttactt ccacatagtc atattttctc agacagggga ggtggttgta
      721 atataaagga agttgtcctg taaatctggg aaaggtaatg ggcagttttc ccgctggatt
      781 gctatttccg aaaataatgt ttgccactgc atttccaccc tcttcgccag gataccaggc
      841 ctctaaaacc gcatcaacac tgtcaaacca gtttaccatt gtaacagcac ttccattgat
      901 aagcacaaca atcacaggcg tccctgtgtc tgcaattgcc ctgattaaat cttcctggac
      961 tcctggaaga ttgagattgc atcgatccct gttttcacct tctgtttcag cagaatttcc
     1021 catcacaaga atcgcaacat ctgccttcgc agcagcatta actgcctttg aaaaacctga
     1081 tttatcctta tcattaaccc tgcagccctc agcaaaatac acccttgtcc tttttgaaac
     1141 gctatttttt attccttcaa aaacactcac aacctttatt ccagagccgc tgtatccacc
     1201 aagtcgtatc gcatctgcat ttggtcctat aacagcaatg gaaccaatgt cttttgatag
     1261 tggcaatatt ccattatttt ccagtagaac catacattgt tcagccacct taagggcaaa
     1321 ttgcctgtgt tggtcgcaat cacagattaa cgatgcctgt gcagggtctg cataaggatt
     1381 ttcaaaaagt ccaagccaga ttttgccctt caataccctt tccactacac ggtcaacagt
     1441 tgcctcagaa acccttcctt cctttacaag tcgtgcaaga tgtgaaaaac atgaagacgc
     1501 aggaatttca atatcaagcc ctgcctcaag agcaagtttt cctgcctcag ccactgttga
     1561 agcaaccctg tggcttccat acaaacccca cacacttcca tagtcagaca caacaaatcc
     1621 ttcaaaaccc cactggtttc ttaaaacatc tgtaagaagc cacctgtttg aagaagatgg
     1681 gatgccatta attgagttat atgccgccat caatgaccac gcctttgctt ccataatact
     1741 ggctttaaag gcagggaaaa atacctctct taaaattctt tctgaaaaaa atactgcgct
     1801 actatctctt ccgccatctc cagcaaaatc agcagcaaaa tgcttcgggg tcgcaattac
     1861 cttttcgctc tgcaatccct tgatgaatgc aacagccatt ttagaagtaa gatacgggtc
     1921 ttctccatag gtttcttcca cccttccatg tcttgcgtct cttgcaatgt tgattgtggg
     1981 cgatagtgcc tggtgaattc ctcttgatct tgtttccttt ccaataattt ttgctacctg
     2041 atacatcata tcagggtccc aactgcttgc cagagcgatt gactgaggaa aactcgttga
     2101 tttttttgcc atacatccat gaatgcattc atcgtgaata atcactgaaa tttttgaact
     2161 gctttcttct aatgcaattt tttgtatttc attggaaatt cttgttccct cctctggacc
     2221 aaatggtctt aaaatgggac taacctgtcc tgcaccattt tttaaccgag caattgcttt
     2281 ctcctttgaa aaatgctcct tttcaagtaa ttcattcctc tcaatgcagg tcaactgtgc
     2341 caccttttct tcaatactca tttttccaag aattttttta atcctttcat ccatagtccc
     2401 ccctgataaa ttttttggtt tattcaattt ttcactcaca aggtttaaca tattgaaaaa
     2461 atttgtcaag ggtagaattg aaaaatgaaa aacaaagttt tttataacaa aaaataccag
     2521 ggttgctatc taggtctgaa attgcctttg gggttaggtt tgtggttttt ctggaaactt
     2581 gaatgtatct ttattttgct cccaccaggg atgttttttc tgggaattta agtctgtttt
     2641 tacttgcctt ttgtgttttc cctcatataa tcagaaagaa cttttgtaaa ttgagattta
     2701 attttatatt tacccaaaga tttgtcataa gtataatgaa tttctgtaat ctctcctgca
     2761 taataactga tagaaacaac ctctaaatcc ttctcagtag tttgaaaaag aagttcacca
     2821 tctgtgatta gtttttgtgt aaattctttc ccattccatc tccaaatttc tattactcct
     2881 ctcattgcag tatattctgt tcgagaaaat ataagttcta aaaacccatc tccatctata
     2941 tctttagcac caaaaacata ccatggatat tcaatttttt ctttttttaa agttgtaggt
     3001 aaaaaattta tatcaaacaa gttcccgtat cccttacttt cccacatttt tttaatttca
     3061 aaaatagcaa ccctggctct atcataaaat ctttcatcag caaccttttt accatctttt
     3121 tctatccagt aatcatgtgg ggaagtgtaa taggcaacta taatctcttc ttttccatct
     3181 ctgttcatgt cagcagtaat aattcctttt ttagtctcat atgtgatttt aacaggatta
     3241 atattaagtc gttagaacga tatcttatta ctggaatttc tgctattatt gaaccatgag
     3301 gaagaagttt gagaatttct cttccaacat cttcaaaact ataactaatt tatacgattg
     3361 acatatttaa ttacatcagt atatccttct gtattcttga aatcttctgg caaatcttgt
     3421 ttcaaatagt aatccttaaa tttttcaaga atctctttac ttttagcaga taattcaggt
     3481 ttttctaagt aattcttcag tttttctact atatttaaga cctgtttatt catttccccg
     3541 tatatctctt ttttgttttc catcttttct atccttggaa agaatttact ccatacatcc
     3601 cataaaaatt gcataactat tactttttct tcttgtggtt ttttggattc ctctaaaaat
     3661 tttagagctt catttatagc atctttctcc tggtgaaaat ttatgttttc taaataagag
     3721 aaaattcctc ttatcatacg gcctttcaaa gattctcgca ttttgttttg atatttgtga
     3781 aaatataaat aactttttaa aagcgtatcc gaatttgttt taatatctga aaaaatagag
     3841 gcaaaaatcc acgctgatga ttctggttca attttatcta tttctacacc taaaagatat
     3901 gctttctctt gctgtggagc aagaatttcc caaaattcct ttgctttttt ctcccatatc
     3961 ttatcataca tatcaagttc ttctttatat cgttctgctt gttctttatc ctttgggttt
     4021 tttacatcaa aactcggtcc tacagattgc caatatttta tactaatttc tttgcgttta
     4081 gcatctattt cttttaattc cagatatcta ttccattctt cttctgtaag aatttgttta
     4141 atgttttttt ccacatctgt aatatttgaa tcttcacacc aacataagtt ctctctaaac
     4201 aaagataaaa aatgaaaatt aaaagatgcc atgagtaaaa ttgaactaaa aaataaaatt
     4261 aatttcaaca ttttttacct cctcctcttc tattcatgaa attctttatt tctccaaata
     4321 ttataaagga aattacataa acaaaaactg gtacaatgaa aagatgaaaa aaagattctt
     4381 tgggaataaa aaaacttaca gcataagcag gtaataatga acataaacct aaaaataaaa
     4441 tgtaaaacaa taaagtaaga attttcttta atgatagaga aattttttgt acagttttta
     4501 aagtgccaaa caataaacca gaaacaatac ccccaaatac tccgaaataa gaaacccaac
     4561 ctacaaaatc acttataaaa tattcattta gttcatctga gtcaagtaaa ataattgttt
     4621 caaaaaatga tacgagcaaa actgaaaaaa atacaggtat aaagaagtaa tacccaaaat
     4681 ttttttcctg tatcaagaca tgtttaagaa atatcacaga caaaatgaaa aaccaaaaat
     4741 gtttccagaa gaaaactttt gtaagattta tgatatggga gtgcgattca aaaatcatgg
     4801 gcattattgt taaagaatag tctaacccaa ataaacccaa actaattaca aatataataa
     4861 tacctttctc ttttataatt cttctatctc tctctatgaa ccaccaacta ataacatagg
     4921 agaatcctgc agtaagaaat aaaacaataa acgttaacat acgcattttt tccacccatt
     4981 ccaaaaaata acaatttctt tttgttcata tccttttata aaaactatgg agaatacaac
     5041 ttcctttctt gttgagacat tctttggtca cgaggtgtac aatacttatt atgatatttc
     5101 aggtgatctt gatccgacat tatattccaa tatgtgtcat tcccaaaaag tctaacttct
     5161 gcattttcat aagcaacttc aggaactcca tcacatctta tcctttgaat atcagaaatc
     5221 accccgtccc aattatcata aggaataact acgtcaagat aaggaaaatc catatattgg
     5281 atgcctatca tttgagagca attattaata atttgtatac tgctccccat taagggtaga
     5341 tggaaaataa ggtagaatag agtgatctga aatggggcag caggacaaca tcacatcctt
     5401 ttttataaaa tcacctcata ctatttccca tctttataaa attatctcat actacccccc
     5461 tctttataaa agaggggctg gggagatttc ttaaaatccc cctttgtccc ccttttgtaa
     5521 agggggagaa cgagatgagg gcttttataa aggggaaata tgaaaaaaat ttcacaaagg
     5581 ggaaaattaa ctcccctctt tataaaagag gggctggggg agatttctaa atccccctta
     5641 atcccccttt tgtaaagggg gacatggaag aaggaatttt gtaaggggga aaattaaagg
     5701 aggaatttgt aaagggtgag tatactgctc ctcattatgc tataataaag taaatgtgga
     5761 aaagtttatg caatggaaac ccttaggaaa aggtctgttt gcgggcacaa aaacagttga
     5821 aatccgttct atctgtatac ctgatgaaga tggtaatctg aaaaggcgaa ggacaacaag
     5881 ttgctggaat cctgcaaatc ctaaattttc aaaaacccct gcaattggtc gactgattaa
     5941 ggatgaatca ggtaaaatag ggatactggt aagcggaaaa catggaacac acatcaaaat
     6001 tggaaaattt gcaagtgcta ttccgttcgt atttattgca atcaattgca ttgcaaaaaa
     6061 accaagagca atgttattaa aggacttacc tgttgaatta tactctgagg gaaacctaat
     6121 ttttgcaaga gaaaaatgaa acgaaaactt attttatgca tcaaaagaga aacagggagg
     6181 aaaaatggag gagaaaaaag tgtataagtg tgaaagtgat attttgtgcg gggtaaacag
     6241 gccaaaagac actgcaaata tgactgactt tgaaaaaaag catgttcctg tcatcactgc
     6301 gccggataag gtaaaaagag acgaagaatt tgaagtgaca gttgaggttg gaaaacttgt
     6361 tccgcaccca aatgaaccag gccatttcat tcagtgggtt gaactgtaca gtggagatac
     6421 attcctcgca cgggcatatt tcaatggtgg ttctgcatct ccaaaggtca cattcagggt
     6481 gaaactttct cacagccatg gatttctcaa ggcatgggaa atgtgtaatc tccacgggct
     6541 gtggcagggt gaaaaaccaa ttgaaattgt tgactgaaag gtgaaaaatg gcaaccaaaa
     6601 tcctggtgct gtactacagt atgtatggaa atgtgtttca gatggcgcag gaagtggtaa
     6661 aaggaattca acaggtgcag gatgctcacc cactcttaag aaaggtgcct gaacttcttc
     6721 ctgaaagcat aattcaaggc aattcagaca tcaaaaaggc gcaagaactt caaaaggatg
     6781 tacctgttgc aacccttgat gatttgaaag aatgtgacgg gcttttgata gggactccca
     6841 caagatttgg aaatatgtgc agccagatga gaaactttct tgataaaaca ggtccattat
     6901 ggcttaaagg agcactcatt ggaaaacctg ctggtatttt cacatcttct gtttctcttc
     6961 acggtggaca ggaaacaacc tgcctggcaa cgatgcttac tcttttgcac cacggtatgg
     7021 taattgtggg aattccttca agtaacccgg atcttatgaa aacaaactca ggcggtacac
     7081 catatggagc aagccatgtt gttggaatgg aggaaaattt caggccactt tctgaagaag
     7141 aaaaaaaatt gtgcaacttt ttaggtaaaa gggttgctga gatcgcaaaa aaattaaaaa
     7201 ctaactgttg aaaggaggta ttttaatgaa aaaaatgaca gaagcaaatt tgaaggcagc
     7261 gtttgctggt gaaagtcagg cgcacatgaa atatctcatt tttgcgaaga aagcagaaga
     7321 agagggaaag aaaaatgtag caagattgtt tgaagccatt gctcttgctg aacaggttca
     7381 tgcaacaaat cacctgaagg cgctcggtga agtcggagat acagtagaaa atctccagac
     7441 agcgatatca ggagaaactt acgaagtaga agaaatgtat cctgcgtttg atgcagttgc
     7501 aaaattgcag gaagaaaaag cagcgcaaaa aagcatcaat tatgcaattt ctgctgaaaa
     7561 ggttcacgca gggctgtatg taaaagcaaa acaggcagtt gaatcaggca cagatattga
     7621 aattggtaaa atttccatat gcaaaatgtg tggctggacc attgaaggtg atgcgcctga
     7681 tcgatgtccg ctctgtaatg caggcaaaga atatttcaaa acattttaac aataactgat
     7741 taaaaggagg aaaatatgtg tggatgctgc tgttgtggtt ccagaaagaa agagaaaaaa
     7801 gagtatgaat gtgcggttaa tacggacaaa tgcccaaaaa agacagtgga cgaaggtgag
     7861 ccagtaccag tttgctgcgg caaacccatg aaaccagcaa agtaattcac tgaattgatt
     7921 aaaacaggtg aatacacctt cttgtgttga taggaaatac aaatgtcaga ttgacgggca
     7981 ggtatatcat tttgtgctgc ggagtcctga aaatctcagc aagagacctg tactgctgat
     8041 aactattgct gctgactgcc atcatacatt gaatcatcct gactacaaca tcatttcaaa
     8101 tgtatttctt gctgcaggac atcgtgttat aggttttgac ctgccaaacc atggcgagca
     8161 tatagacaat tatggtgaag gtcttggtgg ctgggcaaat gcaatttctg atggtgtgga
     8221 catttttgaa aaaatcagaa aaatcggttc atcaatcatt gattttgcca ttaaagaaaa
     8281 acttgcctgg gaaaatacca ttgttatgac aggtgtttcc cgtggtggac tttcagccat
     8341 gcatatcatg gcttatgatg aacgtgtgta tgcagcagtt gttcatgctc cgtgtactga
     8401 cttaggaaaa atcagagagt ttaaatccct gaaagataat cctgttattg cctcttcaaa
     8461 cgttataaat cttgttaacc ggcttgcaaa caggttttta ctcatcacaa ttggtgaaaa
     8521 agatccaagg gttgatgaaa aatcatgtct tgaattttac gcaaggttat tcgccacatc
     8581 agatacaata aagccctcta tttttattct tgagggtgaa acccacggaa acactgcttt
     8641 caatgacgca gcatacattg ctggcgcatc ctttcttttg gaaaaaatct ccctgagaat
     8701 aaaaagaaag gtcaacgatt gaccttaaga tacgggtcct ggtgctgaag ataatttttg
     8761 atgtaatctt ctattgcctg ctcaagggta aaaagatttt cttggtatcc tgctgcgcgc
     8821 aattttgata tgtctgcctg agtaaaatac tggtatgaat tttttattga ttctggcatt
     8881 tcaatatact gaatgttttc tcttttccca agggttttaa atgctatttt cacaatgtca
     8941 ttaaaacttc ttgctttgcc agtcccaata ttgaaaattc cattgattgc tgaatgaaaa
     9001 tagatgaaca gtgtcatttt cacggcatcc tttacataaa taaaatcacg tttttgctca
     9061 ccgtccgggg aatccttcct gtaagattta aaaagttgcg ctgtacctgt tttcaggatt
     9121 tcaaagtatt ttttcacaac gacgcttttc atttctccct tatggtactc attcggacca
     9181 aaaacattaa aaaatttcag tcctgcaatt ttactgagca gttgattctt gtaagcccac
     9241 aggtcaaaaa ggtgtttgga ataaccataa cgattcaatg gtttcagcaa atgtagtttt
     9301 tcttcattgt ctgaaaactc tggaccattt ccataggtgg ctgctgatga tgcataaata
     9361 aacctgcaat ttcgtgcatt tgcccatatt gcaagttttt ttgtgtagtg gtaattgttt
     9421 tcaagtaaaa aactgaagtc cttttctgtg gtatctgaac atgctcccat atgaaggata
     9481 cctgaaatac aaccattgaa agtacctttt tcaagtttta agatgaactc gtctctgtca
     9541 aaaatatcag caaatttaag atttacaagg tttttccatg attcagattg accgggtata
     9601 tcaactacaa taatgtcatc tattcccatt tcattcaact gccatacaag agcacttcca
     9661 ataaaaccca ttccacctgt aacaatatgg tactttttca tgattttcag atacccctca
     9721 ccacagtggt caacacatat ttctcattga aggcataaga ataatgaaca ttcctcactt
     9781 tttttcctga atgaaacaat tccaggttat caccatccca caaaatttca atatcattaa
     9841 agtacgtttt tttctgaata ttttctggaa gtgtcttcca gaatgtttct ttcattgaaa
     9901 atccaattga aaaatagatt tctttttctt caggaaagct tttcctttca cgctgtgtgt
     9961 aaattttttt caaaaatctc tgcccatgac gtctcagggc aatgttaatt cgagcccttt
    10021 caagaatatc aatccccaca gcaagttttt tatctgccga agataatacc tttggacttc
    10081 tggtacttgc cttttttgtc tgcatatgac ttttcgcact cgcctgttgc ctttaagaat
    10141 atgacctggc atattccttc gccagcataa accctgcctg gcacaggtgt gaggtttgct
    10201 atcatgatag taatatggcc ttcccattca ggttcaagtg gcgtgacata taccacaatt
    10261 cctgaccggg cataacttga ttttccctgg caaatcccaa gcacatttct tggtattctg
    10321 aaatattcta cgcttctccc cagaacaaaa cttcctggcg gtatttcaca aactttcccg
    10381 gtaaaatttt tgaaagaacc aggatcaggg tttttcgggt ctatggtatt gcttcctgta
    10441 aaaactttga attcatcagc aagcataaag tcataaccgt aagaagaaac cccataactc
    10501 atcacacctt tttttacact tgattcaaaa aagggctcaa tcaatccctt ttttgcgttt
    10561 cttttaatcc atttatcagg tagaaccatt ttttatctta tatccacaac aattccgtga
    10621 tgaaaataaa taatgtgagt tctgttattt tttctataag cccatgattg ataaaccttt
    10681 ccattgataa tcaattcatg ctgtaactca ggttctcccc agcttgcaat aacttcttcc
    10741 atcgtcattc cgtagataac cctgtgctga tcgattgccc tgacaacagc aactttattc
    10801 attggataat cagtaatcat actcctgcct tctctttcag ccctgaaacc aggatgaatc
    10861 gcacaaccag taaaaacaat cgcaaatcca atgaatagca cacttattat ctttttcatg
    10921 atttttcctt ttgggttatt ttatcaaatt ttaaaaaaca gtcactttct caaaacgagc
    10981 caggcaaaaa atgctgctcc aaaaccatta tcaatattga caacaacaat cggcgcgcaa
    11041 ctattcagca tcgtgagaag cggtgcaaat cctttcaaat ttgttccata accaacagaa
    11101 gtcggcactc caatcacggg tttcccggtc attcctgcaa tgacaccagg taaagcccct
    11161 tccatcccgg ctatcacaat caaaatctta cactgattaa gtttcttcaa atgtggcaca
    11221 atcctgtgaa tgccgctgac gccaacatca taaattctct caactccttt accgagtatc
    11281 tcaagtgtca cagctgtttc ttctgcaaca aaatagtcag aagttccagc gcacagcacc
    11341 gcaacttctt ttttcctttg tttgtttatt ggtttgccag taagaattct cgcgctttca
    11401 tgataaacaa gttctggata tgttttcagg atttcatcaa ccttttctct agaaatcctg
    11461 gtaatgataa aatttttgta tttgcctgaa agttctctga taatggagac aagatggtca
    11521 acactttttc cttcaccata cacaacttca ggaaagccaa cccttaatgt tctgtgatgg
    11581 tctatttttg agtgtctgag atctgaaaat ggtaaaattt ttaactgttc aaacacatcg
    11641 tcaaaagaat agtgaccatt tttaaatttt cgaagtatct ggtaaagttc ttctttttcc
    11701 ataatgttat attttacagt atctgcctgt gttcgtaaat ttatggaaat ttttttgagt
    11761 ttggttaaaa taataccatg accaaaagag aaatagttat aaatgcgatt aaccatcggc
    11821 agggaccaac ggttccatat tttataggtt tcacccacag ggtcaggctt caaatggcgc
    11881 aatactataa tgaccctgaa tttgagcgca agattggtaa ttatttctgt cagtgattat
    11941 atggttgcac ctgctcattc cataccatat ggagcaaaac ctgaaaatat tgcagcaatg
    12001 atagaggtac ttcaacatca ataggcaggt aactatgaat ggaaaacaga gaagtcttgc
    12061 agtattaaac catcaaaatt ttgatagata tccattgttt gacatcctta ccaatgatgc
    12121 tgcaatcgaa tattatgcag ggaaaaagtt aacagtggaa aacgggaaac aggttgttca
    12181 cgctgcctta gacaatatgg ttgatgcaac aagatggatt attatgtacc cgcagaagga
    12241 agaaaaaatc atcgcagaag atggaacaat tattgaacag aaaagatgga ctgcgtggta
    12301 tcacagaaga tttactctca atcttgatga tgtggtaaaa aatatcaaaa atgagattga
    12361 gtatttttca aatccagaaa atattgaaaa agaagccatt gctcttgacg cgagaattga
    12421 gcaacttgta caaataagag atgaattcaa caatatattt ctttttggaa attacctgat
    12481 taaaacaggc attcagcttt atgcaggtat cggacttgag ttattttcat acctcatcac
    12541 tgattatcct gacatttttg accagtggat gaatcttgga actgaattta aggtacaggt
    12601 tatcaacagg gcaaagaatg tttctgagtt tcctgccatt tttgactgtg aagaccttgc
    12661 ttcaaaaaat ggtccacttt tttctcctga gattatgaag aaatattttt atccccattt
    12721 agaaaaactt gttgatgtct atcataaaaa gaatgtaaaa ttcatttttc atacagatgg
    12781 caatgtcatg ccaatccttg acaacctggt ggcaactgga attgacggac tcaatcctct
    12841 tgaagtaatc gcaggtatga atctgaaaga aataagaaaa agacacaaag atttaatcat
    12901 gcttggaggt atcgattgca gtcaacttct accatacggc acacgcgagc aggttcgtca
    12961 ggcaacaatt caggcgatac aggatggggg tccatggtac tttcctggct caagcacaga
    13021 attacatgat aatatcccgc tggacaatat cagggtaatg gtggatacta taagggaaac
    13081 aagactataa accatatctg tgtttatttc gtcaaacatt gacacatggg aatgaaaaaa
    13141 tattatttca ttatggtgct gcttgtatgc ctctgcggat gcagtgtttc ttattatgaa
    13201 aagagcgctg acagagaggt ttcttccatt ttaagggaaa aacagcaacc tgctgaaaaa
    13261 gggaaaatca aagattctgg tttggtagat gagaccagac atgaaacaat ttttcttaac
    13321 ttaaaggatg cccttattct tgcagcaaaa agcaaccgcg aatatcagac tgcaaaggaa
    13381 gacgtgtatc tttcagccct ttctctgaca tatcagcgtt atctctacgg cgttcagaga
    13441 gacgcatctg gctcagtaga atggaattct gcttctggac aagatatagt ttctggaaat
    13501 ctcaatcttt cccttgtgaa gtggctcgcc cagggagcac aaattacatt tgatatcggt
    13561 cagtatctga caaaatatct cacaggttcc aaggaaaaca tctatcaaac tgcattgagt
    13621 ttaaatatat ttcaaccatt atttcagggt gctggacgcc tggttgccca ggaaaatctc
    13681 gttcaggcag aaagaaatgt tgtctatcaa ataaggtcct tcctcagata tcagagaagt
    13741 ttttcagtcc aggttgccca gaaatatttc aacattattc ttgcgcaaaa caatctggac
    13801 aattactgga agaattatgt ctttctaaaa gataccaggg aaagagtgga aatgctggcg
    13861 gctgctggaa ggattccacc ccttcaagca gaccaggcac gacagaatga atatcgtgca
    13921 taccagcagt ggatacaggc aggtaacaac tacaaacagc aggaagatga attcaaaata
    13981 ttcctcggtc tttcaccaga aactgttatt gtacttgata aaaaagcatt ggaactattt
    14041 ttagaaaatg ggttaaatcc gatcgacatt aacacagaaa ccgccataga gtatgccttg
    14101 agcaacagac ttgacctgat gacttattca gattatgttg gcgatgctga aagaaatctt
    14161 gctatcgcta aaaatttact cagaagtaga attgacttct ccggcacagt ttcttcatca
    14221 acagaagaaa aatcaacatt tgaccttgaa ttgaaacaac cctcctactc agcaggcata
    14281 gattttgaat tgcctaccgg aaaaatttca gacaggaata gatataagca ggcattgatt
    14341 aactttgaca gaaaaaagag ggattttacg ctgaagaggg agaacataaa acttgaaatc
    14401 ttaaattcta taagagacct tgaagaagca taccagagtt acattgtgca gaaaaacagt
    14461 ttacaacttg caacaagaag ggttgaaagt acagatttaa tgcttcaggc aggcagagca
    14521 acaacaagag atttacttga agcacaggaa tcgtttctga gtgcaaaaaa ttctctgtct
    14581 tcagcaattg tcaattacct gttatcctac cttgaatttc tcaaaaatac agaacaactg
    14641 caacttgaca gcgatggtgt ctggaaagga gacctgtatg aaaaaattat tggtgaaaat
    14701 cccgaaat
//
LOCUS       DAORYU010000007        13923 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_3469_length_13923_cov_3.902077, whole genome shotgun sequence.
ACCESSION   DAORYU010000007 DAORYU010000000
VERSION     DAORYU010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13923
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3469_length_13923_cov_3.902077"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="PLW07_00565"
     CDS             complement(<1..293)
                     /locus_tag="PLW07_00565"
                     /inference="COORDINATES: protein motif:HMM:NF013263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyphosphogluconate aldolase"
                     /protein_id="HOL49049.1"
                     /translation="MHKDLSPLSGTGICAIIRIKTQNVDIESLVNSLVHGGINAVEVS
                     FDTIDAISLIKSIRNYADRKVLLGAGTILNTDAAQSAIDAGAQFLVSPHLDINL"
     gene            complement(286..1455)
                     /locus_tag="PLW07_00570"
     CDS             complement(286..1455)
                     /locus_tag="PLW07_00570"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOL49050.1"
                     /translation="MKITEGIFSVGSVDWDLKEFHGYSTPYGTTYNSYLITGEKNILI
                     DTVKPTCTHEFLQKIERIVPVEKIDYVISNHAEMDHSGFINWILSKSNATLICSPKGK
                     ENLEKHFHTKYERILIVENGQTMKIGPYTYQFFLTPMVHWPDSMCTYLIEKKMLFSND
                     AFGQHFASSKYFVDEVGLEIVLREAKKYYANIVNPYGNSVKKVLAALSTLQIDIILPS
                     HGMIWRNSQHIKTILDNYNRWASNQPGKKVLIVYETMWGSTKKMAEVCYVEAVKKTIE
                     AEVFNLQIKDISDVVAEMPDAEMVLFGSSILHGQILPKMAGLITYLTGLKFINRKAWT
                     FGSYGWAKGSFNRFETNIKDAGFDMPYSGFYIQFVPDESSLEFLRNELNSRIFNA"
     gene            complement(1471..1626)
                     /locus_tag="PLW07_00575"
     CDS             complement(1471..1626)
                     /locus_tag="PLW07_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOL49051.1"
                     /translation="MKYVCKVCGYVYDPEKGDDTQDINPGTLFEDLPESWTCPECGAG
                     KEEFEPL"
     gene            complement(1641..2324)
                     /locus_tag="PLW07_00580"
     CDS             complement(1641..2324)
                     /locus_tag="PLW07_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOL49052.1"
                     /translation="MRIQTSVLSNIVRTDSARSVKVIKPESFSFLPGQFFKVYANDGF
                     RYLSASCSTRKNYLEFTKKITQSDFSAWFKSLNKDDVITIDGPYGKFVIEPDDDKILF
                     VAGGIGITPIFSMLEEAAISNDNERDYILFYGNKTINDIIFYNELQEFTKKIQLKIFH
                     FLEEEREDEINCHHGYIEIQKIQKFLPDIADRTIFICGPPTMVNKLDNDTKKFKIAKK
                     VKVERLVGY"
     gene            complement(2339..3748)
                     /locus_tag="PLW07_00585"
     CDS             complement(2339..3748)
                     /locus_tag="PLW07_00585"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOL49053.1"
                     /translation="MGGIKKRIKKYSAKQTLSGVLKILPHLSDQNVLRILSVAEKISV
                     NPAFKERVLVIEEKIKQKHPTLEIVRRLNNQLSSVPKKKLITNLFANAMFEGTARRQE
                     IYEQEQWRPPFFFVLSPTMRCNLNCTGCYAAQYTKKDVITTEQIDRIFTEAKELGIYF
                     VTISGGEPFYRKDLPELWQKHNDMFFLVYTNGTLIDAKMAEKLKELGNVAPAISVEGF
                     KEETDKRRGQGVYEKLLEAWNNLRENGVLFGFSATCTKYNTEVIYSDEFIDFMIGQGA
                     LFGWYFQYIPIGRKPDISLMATPQQRAWAREKLDHIRNTRPIILADFWNDGHLAGGCI
                     AGGRVYFHINSHGDIEPCVFTHFTKDNIKDTTIRQALQSNLFKAIQQRQPYSNNLMCP
                     CMIIDNPHVLREIVAETGARPSYPGAEAIITESAKFLDEYAKEIHQLWDPVWIEKYKA
                     GKDLKGIDAEVAARLRNNK"
     gene            complement(3750..4109)
                     /locus_tag="PLW07_00590"
     CDS             complement(3750..4109)
                     /locus_tag="PLW07_00590"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF014332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOL49054.1"
                     /translation="MIYPVCRTKIKGGKVTGKKLYYDGSITIARDILHAAELQPGDMV
                     DVLNLNNGARITTYVIEGAEKTGEICLNGPAARFFEEDDEVVILSICLVTASERHNMK
                     MKIVSLTGNNLRITVEG"
     gene            complement(4114..4956)
                     /gene="panC"
                     /locus_tag="PLW07_00595"
     CDS             complement(4114..4956)
                     /gene="panC"
                     /locus_tag="PLW07_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOL49055.1"
                     /translation="MEIAETKSLIRNIVAEARKKNKTIGFVPTMGYLHDGHASLIQKS
                     KKDGCFTIVSIFVNPLQFGPAEDFDRYPRDMERDINILKKEKTDAVFVPSVEEMYINQ
                     HLTLVNVSRISEKFEGAIRPGHFTGVCTVVAKLFNIVQPDRAYFGWKDAQQLVIIKKM
                     VKDLDFHIDIIPVPTVREPDGLATSSRNVYLSPEERKRALILSKSLQQIGEMVEYKRI
                     FDVNILLKEGEKIIKTENVTLQYLEAVELENFEPVNKIGKNTAIVGAIKVGKVRLIDN
                     IIWE"
     gene            complement(4892..5410)
                     /gene="folK"
                     /locus_tag="PLW07_00600"
     CDS             complement(4892..5410)
                     /gene="folK"
                     /locus_tag="PLW07_00600"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014452953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOL49056.1"
                     /translation="MAEVLIALGSNLGNRRKNIFNAIEKIKEGIEIMRISTLYRTEPQ
                     EGVSGNWFLNGVLSGKTCLSPEELLEFLQSVEQEMGRPENHKKNTARTIDLDILFYDN
                     CFIKKPHLRIPHPKINTRDFVLKGLVEIAPDFEHPEVKTTIKQMWKEMTNGDSRNKVP
                     DKKYRCRSKKKK"
     gene            complement(5403..6329)
                     /locus_tag="PLW07_00605"
     CDS             complement(5403..6329)
                     /locus_tag="PLW07_00605"
                     /inference="COORDINATES: protein motif:HMM:NF021646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49057.1"
                     /translation="MQSQGCWIKNFINPVPELHYLNFFQYSEKKLPGLSVIRAISKGI
                     ALDAEHTYFLADFMTVDGTRTVGNADISEHETEILAKEIQGTFDYFEFDVVGPGQILT
                     KFAGRYPYTNWKFPFQIIGLDYKTFLPQDGVLRTPVRIIENSCRLLEKHPVNRVRIDL
                     GENPANFLWLHSQSRDFEKSGAISKKIGKRAIFCSQQDSIIDDIARYLGFEVVNQINF
                     DSGDDAIVWINFKPQSFDLIGLIHAWESIDREFLRLFRKNDTRIVIEFNTGFYHGKDL
                     SLNFLWPVKGINPIKRTLMAKKLPGNFLLYDG"
     gene            complement(6431..7720)
                     /gene="purB"
                     /locus_tag="PLW07_00610"
     CDS             complement(6431..7720)
                     /gene="purB"
                     /locus_tag="PLW07_00610"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008195684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOL49058.1"
                     /translation="MIPRYTRKEIGDIWSDETRFSTYLEVELAVIEAYQELGVISSSV
                     AQHIKKHAKIDIKRIEEIEKQTNHDIIAFVEQIGETIGEASRYFHYGMTSSDLVDTAT
                     NLLLIKATRILLRDLEQFSETLRQKALHYKDLVQIGRTHGIHAEPITFGFKIAGWFFE
                     TKRNIERLRAALKEISVGKISGAVGTYSNINGDVEKLACKKLGLEPEIFSTQIISRDR
                     YACYISTMGVIACSLEKIALQIRLLQQTEIDEVAEPFGKGQKGSSAMPHKRNPILCER
                     ICGLARIIKNNVSVAMENVALWHERDISHSSAERIILPESTILLDYIISLTNKIISEI
                     EVFPESMEKNLNLTHGLIFSQRILLLLCDKGLARKQAYEIVQKNALKSKKEKIELMSL
                     LLQDAEVRSHLTEQEIRQCFDLKWYLRNITHLFDKLK"
     gene            7792..8712
                     /locus_tag="PLW07_00615"
     CDS             7792..8712
                     /locus_tag="PLW07_00615"
                     /inference="COORDINATES: protein motif:HMM:NF014610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HOL49059.1"
                     /translation="MKTKAIGLVSGGLDSMIATKLMIDQGIEVIPVKFTSPFFHSEDI
                     KKEPIDFLGLKLRLIQVGEEYLSIIRNPEFGYGAGLNPCVDCKIYMLKEAGKIMKETG
                     ASFVFTGEVLNQRPFSQKKIFFELVNRETLLKGRILRPLSAQLLEPTEPELAGFVDRS
                     KLLAISGRRRMQQVAIAEKMGIKDYPQPAGGCLLTDKIYAEKLKKLLNYWDECTLNDA
                     EIIKHGRVFWAGDVLLVVGRNKEENELLRTIAKKNDIIVQLKSVPCPLTVLRGKNIDN
                     SAIEYAKDLIVYYTKRAGRDKSEFSVEKIQ"
     gene            8752..9783
                     /gene="rlmN"
                     /locus_tag="PLW07_00620"
     CDS             8752..9783
                     /gene="rlmN"
                     /locus_tag="PLW07_00620"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOL49060.1"
                     /translation="MKENIRNFSLDEVERVISSMSEPRYRAAQVFRWLYQKGAESFSE
                     MSDVPEKLRFRLNQKYTIDSLQSDHMFVSEDGTKKFIFKLRDNRYIESVMITDENRNT
                     VCISTQVGCKYNCVFCASGKMGFIRNLDVSEIIGQILYIKLKENVKISNVVFMGMGEP
                     FDNYDALIKAISIINAREGLNIGARKITVSTSGIVPGIKKFADAGWQVRLSVSLHAPD
                     DATRSSLMPVNKKYPVAVLLDACNYYISRTGRRITFEYILIAGVNDKENHLSGLERIA
                     KKLHADINVIPYSRIKNCGFTSPSAEKIRWFVGSLRKKGIKATVRNSRGSDINAACGQ
                     LAGARSFCE"
     gene            9776..10438
                     /locus_tag="PLW07_00625"
     CDS             9776..10438
                     /locus_tag="PLW07_00625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOL49061.1"
                     /translation="MNKAKNYCAIAIDIDGTIVQPVSSWRYIHEKLGKWDVLAYRYQD
                     MFLAGKITYRQFCKLDAAHWKGIPEKKISSLFKNVPYTRNAKRCLLKLKDMGFQLIAL
                     STGLQYIPEKLQDEIGFKIVVSNRLLSRRGILTGGVKINISHGEKGIVLRKILKKLNL
                     SSDQVICVGDSDGDIPMAKMCGFSIAFNSSSQKLNKVVDFVCESQNFIEVLSVIEKLI
                     SQ"
     gene            complement(10424..11782)
                     /locus_tag="PLW07_00630"
     CDS             complement(10424..11782)
                     /locus_tag="PLW07_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HOL49062.1"
                     /translation="MPFSIEEIYETARMILFEKLDIRTTTLGVNIKDCIHSNFSRFQK
                     NVHKKIVRYGKSLVKNAREIEIKYGIPIVNKRISITPISLVMETHATSKKYLDMAKII
                     DLAAKESEIDYIGGFGALVHKGMTEAEKILIDCMPDVIASTERVCCFLNAGTTSSGMN
                     MDVVKIVGEMLNKTARKTHNGIGCARFVIFVNAPEDNPFMAGAFHGPGESEASLNIGI
                     SGPGAVRSVVEKNRECDMTELSEIIKRTVFKITRAGELVGRELAKKIGVSFGIVDISL
                     APTPSPGDSVAEIIEAFGIEKIGAPGSTLAIALLMDAVKKGGAMASGNVGGLSGTFIP
                     VSEDAGMAEAVASKALNIEKLEAFTAVCSVGLDMIAIPGDTPPDTISAIIADELAIGI
                     INNKTTAARIIPVPGKKAGEKVNFGGLLGEMPVMSVNKFSSSKFIKRGGRLPSPVTSL
                     RN"
     gene            complement(11785..12063)
                     /locus_tag="PLW07_00635"
     CDS             complement(11785..12063)
                     /locus_tag="PLW07_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015733234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOL49063.1"
                     /translation="MKKELVFITVIGKDKKGIVARISMTLFKRNINIEDITQKIIEDH
                     FIMAMLVDIKQANCSVETIVKDLEKIGKQMGLIIQVQHENIFKMMHRI"
     gene            complement(12093..>13923)
                     /locus_tag="PLW07_00640"
     CDS             complement(12093..>13923)
                     /locus_tag="PLW07_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HOL49064.1"
                     /translation="PAVVTIGLALGVQRMAKKNVLIRKLPSVETLGCATTICSDKTGT
                     LTKNEMTVKKIYADGTTFSVTGAGYDPQGNFLINDRPVLPEQFPGLNRSLLCAVVCNG
                     ARLTKHNNMWIVTGDPTEGALLTVAAKAGKLKEEIENTFQIVDEIPFDSERKRMTVIV
                     RHNQNLVSFIKGAPDIILNLCSFIEENNSIKHLDETGKKLILDITDSFAKQALRVLAT
                     GYKNIEPDADISEKTVETNIVFTGLFAMIDPPRDEAKLAIEKCKKAGIKVKMITGDHK
                     LSAIAIAKELSLIQNEHAVIAGDRIDSLDDNHLQETVNKVSVFARVSPHHKLRIVRAL
                     RENNEIVAMTGDGVNDAPAIKEADIGIAMGITGTDVTKEVSDMIITDDNFASIVAAVE
                     EGRAIYENIKKFVHYLLSCNAGEIMLMFFASFLKTPVPLLPIHILWINIVTDGLPALA
                     LGVDPADPGNMEKPPRKPDEQIVNRESGKMIILQGFFIAMCALATFLYVIYIEKAGIE
                     RARTMTFFVLTCSQLFHSFNLRSDVHSIFKLKFSTNMKLVYAVILSFLLQLAAIYLPV
                     SKTIFRTVPIMGIDIVIAMTISTLPLWMMEIVKLIIRKKGIKS"
BASE COUNT         3993 a         2682 c         2524 g         4724 t
ORIGIN      
        1 aggttaatgt ccagatgcgg gctgacaaga aactgcgccc ctgcgtctat cgcactttga
       61 gcggcatcgg tgtttaatat ggtgcctgca ccgagcagga ctttcctatc tgcgtaattc
      121 ctgatacttt ttatcaggga tatggcatcg attgtatcga aactgacttc aaccgcgttt
      181 attcctccat gaacaaggga gtttacaagg gattctatat caacattctg agttttgatt
      241 ctgataatgg cacaaattcc agtacctgat aatggtgata ggtctttatg cattgaaaat
      301 tctggaattt aactcattcc tgaggaactc aagggaactt tcatcaggaa caaactggat
      361 gtaaaaacca ctataaggca tatcaaatcc agcatctttg atatttgttt caaatctgtt
      421 gaaagacccc tttgcccagc catatgagcc aaatgtccac gcttttctgt taataaactt
      481 tagtcctgtt aaatatgtaa tgagacctgc cattttcgga agaatctgtc catgtaaaat
      541 tgaagaacca aacagaacca tttctgcatc aggcatttca gcaacaacgt ctgaaatatc
      601 cttgatttga agattaaaaa cttctgcttc aatagttttc tttacggctt ctacataaca
      661 cacttcagcc atcttcttcg ttgaacccca catggtttca taaacaatga gaactttttt
      721 ccctggttga tttgaagccc atctgttata gttatcaaga attgttttta tgtgctgcga
      781 gtttctccat atcatgccgt gtgacggtaa aatgatatca atctgcaacg ttgaaagcgc
      841 tgcgagaacc ttcttaacgg agttcccata aggattaacg atattggcat aatatttttt
      901 tgcttccctc aatactattt ctaaacccac ttcgtcaaca aaatactttg aactggcgaa
      961 atgctgacca aaggcatcgt ttgaaaaaag cattttcttt tcaattaaat aggtacacat
     1021 tgaatcaggc cagtggacca ttggtgtgag aaaaaattga taagtatacg gaccgatttt
     1081 cattgtctgg ccattttcaa caataagaat tcgttcatac tttgtgtgaa aatgtttttc
     1141 caggttttct ttaccttttg gtgaacatat caatgttgcg tttgattttg atagtatcca
     1201 gtttataaag ccagaatggt ccatctcagc atggtttgat atgacataat caattttttc
     1261 aacaggtaca atacgttcaa ttttctggag aaactcatgg gtacaggttg gtttcactgt
     1321 atcgatcaga atattttttt ctccggtaat aagataggaa ttgtaggttg ttccataagg
     1381 agttgaataa ccgtgaaatt cctttaaatc ccaatcaaca gaaccaacgg aaaaaattcc
     1441 ttctgtaatt ttcattgaac tactcctcat ttataggggt tcaaactctt ccttgcctgc
     1501 tccacattca ggacacgtcc atgattcagg cagatcttca aataatgtgc ctggattgat
     1561 atcttgtgtg tcgtcacctt tttccgggtc ataaacataa ccacacacct tgcatacata
     1621 tttcattttt cctcctgttg ttaatatccg accagtcgct caacttttac cttttttgca
     1681 attttaaatt tcttggtatc attatctaat ttgttcacca tagtgggcgg accacatatg
     1741 aagattgttc tatcagcaat gtcgggtaaa aacttttgaa ttttttggat ttcaatgtag
     1801 ccgtgatggc agtttatttc gtcttctctt tcttcttcga ggaaatgaaa aatttttaat
     1861 tgaatctttt tcgtaaactc ctgcaattca ttataaaaaa ttatatcatt tatagttttg
     1921 tttccataga ataaaatgta atccctttca ttatcatttg aaatcgctgc ttcttcaagc
     1981 atgctaaaaa taggagtaat gcctatgccc cctgcaacaa aaagtatttt atcatcatcc
     2041 ggttcaataa caaatttacc ataaggtcca tcaattgtga tcacatcatc tttgttcagg
     2101 gatttaaacc acgctgaaaa atcgctttgt gttatttttt tggtaaattc aaggtaattt
     2161 tttctggttg agcaagatgc tgaaagatat ctaaacccat catttgcata tactttaaaa
     2221 aattgtcctg gcagaaaact aaaactttcg ggttttataa cttttacact tcttgctgaa
     2281 tctgttctga caatattgct caatactgac gtttgaattc gcatttaata tggaaatttt
     2341 acttattgtt gcgcaatctt gcagcaactt ctgcgtctat acctttcaga tcttttcctg
     2401 ctttatactt ttcaatccag acaggatccc ataactggtg aatctctttc gcatattcat
     2461 caagaaactt tgcactttct gtaataattg cttcagcgcc aggataggaa ggtctcgcgc
     2521 ctgtttctgc gacaatttct cttagtacat ggggattgtc aataatcatg catggacaca
     2581 tcaaattatt tgaatatggc tgtcgttgct ggattgcctt gaacagatta ctctgcagtg
     2641 cctggcgtat ggtagtgtct ttgatattgt ctttggtaaa atgggtgaat acacatggtt
     2701 ctatgtctcc atgggaatta atatgaaagt aaacacgccc acctgcaatg caaccacctg
     2761 caagatgacc gtcgttccag aagtcagcaa gaatgatggg ccgcgtattt cttatatggt
     2821 caagtttttc tcttgcccat gctctttgtt gaggggttgc cattaaggat atgtctggtt
     2881 ttcttccgat aggaatgtat tggaagtacc aaccgaagag tgcgccctgc cctatcataa
     2941 aatcaataaa ttcatcacta taaattactt ctgtattgta ttttgtgcag gttgcagaaa
     3001 atccaaataa cactccattt tctcttaaat tattccacgc ttcaagcagc ttttcatata
     3061 ccccttgtcc ccttcttttg tctgtttcct ctttgaatcc ttccacactt atggcaggag
     3121 caacattacc gagttccttt agtttttctg ccatttttgc atcaattaag gttccattgg
     3181 tgtatacaag aaagaacata tcattgtgtt tttgccacaa ttcaggcagg tcttttctat
     3241 aaaacggttc tcctccactt attgttacaa aataaattcc cagttctttt gcttctgtaa
     3301 atattctatc tatttgttct gttgtgatga catccttttt tgtatattgg gcagcataac
     3361 agccagtaca atttaaatta catctcattg tcgggctgag aacaaagaaa aatggcggtc
     3421 tccattgctc ttgttcgtaa atctcctgac gtcttgctgt tccttcgaac attgcatttg
     3481 caaaaaggtt tgttatcagt tttttctttg gaacagatga aagctgatta ttcaaccgtc
     3541 tgactatctc aagggtggga tgcttctgtt ttattttttc ttcaataaca aggacccgtt
     3601 ctttgaatgc gggattcacc gaaattttct cggcgacaga taatattctt aaaacattct
     3661 ggtcagaaag atgaggcaaa atttttaaca caccggagag tgtttgttta gcgctgtact
     3721 ttttaattct ttttttaatt cctcccatat taaccctcca ctgttattct taaattattg
     3781 cccgtcaatg aaactatctt cattttcatg ttgtgccttt cgctggctgt aacgagacag
     3841 atgctcagaa ttactacttc atcatcctct tcgaaaaatc ttgcagcagg tccattaaga
     3901 cagatttcac ctgttttttc agcgccttct atcacatagg tagttattct tgctccgttg
     3961 ttaaggttaa gaacatccac catgtctccg ggttgcaatt ctgcggcatg caaaatgtcc
     4021 cgggcaattg ttatgcttcc gtcatagtaa agttttttac ccgtaacttt acctcccttg
     4081 atttttgttc tgcaaacagg ataaatcatt tgattattcc cagataatgt tatctattaa
     4141 ccttactttt ccaaccttta tcgctccgac gatagcagta ttttttccta ttttattaac
     4201 aggttcgaaa ttttcaagtt ctacagcttc taaatactgc agtgttacat tttctgtttt
     4261 gataattttt tcaccttctt taagcaggat attcacatca aatatccttt tgtattctac
     4321 catttcacca atttgttgta aagattttga gagaataaga gccctttttc tttcctctgg
     4381 tgataaataa acattcctcg aacttgttgc aagtccgtca ggttctctta cagtggggac
     4441 aggaataata tcaatgtgaa aatcaaggtc ttttaccatt ttttttataa taacaagttg
     4501 ttgcgcatct ttccatccaa aatatgccct atccggttga acaatgttaa ataactttgc
     4561 tacaactgtg caaacacctg taaaatgccc tggtcttatt gctccttcaa atttttccga
     4621 gatcctggaa acattaacga gcgtcaggtg ctgattgata tacatttctt ctacagaggg
     4681 aacaaaaact gcatctgttt tctctttttt caatatattg atatctcttt ccatatctcg
     4741 tggatatcta tcaaagtcct cagcgggtcc aaattgtaat gggtttacaa aaattgaaac
     4801 aatggtgaaa cacccatctt ttttagattt ctgtattaag gatgcatgac catcatgaag
     4861 atatcccatt gttggaacaa aaccgattgt tttatttttt tttcttgctt ctgcaacgat
     4921 atttcttatc agggactttg tttctgctat ctccatttgt catctccttc cacatttgct
     4981 tgatagtggt tttaacttca ggatgttcaa aatcaggagc aatttctaca agccctttca
     5041 atacaaaatc tcttgtattt atttttggat gaggaattct cagatgtggt ttttttataa
     5101 agcaattgtc gtaaaaaagt atatccaggt caatcgttct tgcagtattc ttcttatgat
     5161 tttcgggtct tcccatttcc tgttctaccg attgaagaaa ttccagcaat tcttctggtg
     5221 acagacatgt tttaccgctc agcacaccat ttaaaaacca gtttccactt acgccttcct
     5281 ggggttctgt tctataaagt gtagaaattc tcataatttc gataccttct tttatttttt
     5341 caatggcgtt gaaaatattt tttcttcgat tcccgaggtt acttcccagc gcaataagaa
     5401 cttcagccat catagagtaa aaagttacca ggcaattttt ttgccatcag tgttctctta
     5461 atgggattga tacccttgac tggccataga aaattcaatg aaaggtcttt accatggtaa
     5521 aaacctgtat taaattcaat aacaattctg gtatcatttt ttctaaacaa tcttagaaac
     5581 tccctgtcaa tcgattccca tgcgtggatg agtccgatca ggtcaaaact ttgtggtttg
     5641 aaatttatcc acacaatcgc gtcatcgcca gaatcaaaat taatctggtt gacaacttca
     5701 aaacccaggt accgtgcaat gtcatctatt attgaatcct gctgggaaca aaagatggcc
     5761 ctttttccta tttttttaga gatcgcgccg cttttctcaa agtccctgct ctgactgtga
     5821 agccagagaa aattggcagg gttttcccca aggtcaattc tgactctgtt aacaggatgt
     5881 ttttcgagca gacgacagga gttctcgata attcttacag gagtccttaa taccccatct
     5941 tgaggaagaa atgttttata atcaaggcct attatttgaa atggaaactt ccagtttgta
     6001 tatggatatc tgcctgcaaa ttttgtaaga atttgccctg gtccaaccac atcaaattca
     6061 aaataatcaa aggtcccttg aatttccttt gcaagtattt ctgtttcgtg ttccgaaata
     6121 tctgcatttc caacagttct ggtgccatca acggtcataa aatcagcgag gaaataagtg
     6181 tgctcggcat ctaatgcgat gccctttgag attgctctga ttacactcag accaggcaat
     6241 tttttttcag aatactgaaa aaaatttaaa taatgcagtt caggaacagg attgataaaa
     6301 ttttttatcc agcagccctg actttgcatc tgacgaaagt tttcagagct acatttttct
     6361 atcggcagac cgtaaatttt atgtttctga aagagtgagt agcctgaatt gaaagagaga
     6421 aatacaaaca tcatttgagt ttgtcaaaaa gatgagtaat gtttctgaga taccatttaa
     6481 ggtcaaaaca ttgacgaatc tcttgttcag taagatggct tctaacttca gcgtcctgaa
     6541 gaagcaggga catcagttct attttttctt ttttgctttt caacgcattt ttttgaacaa
     6601 tttcgtacgc ctgttttctt gcaaggccct tatcgcacaa aagcaacaaa attctctgtg
     6661 aaaaaatcaa accatgggta agattcagat ttttctccat tgattcggga aatacttcaa
     6721 tctcagagat tatcttgttg gttaaactga taatgtaatc aagaagaatc gtggattcag
     6781 gtaaaatgat gcgttctgct gaagagtgac tgatatctct ttcatgccac aatgcaacat
     6841 tttccattgc gacagacaca ttatttttta tgattcttgc aagaccgcaa attctttcac
     6901 agagaatagg atttctttta tgtggcatcg cagaagaccc cttctgccct tttccaaaag
     6961 gttctgcgac ttcgtctatt tctgtttgct gaagcaacct tatctgcaat gcgatttttt
     7021 caagggagca tgcaattact cccatggttg aaatgtagca agcatatctg tctcttgaaa
     7081 taatctgggt tgaaaaaatt tcaggttcca atccgagttt cttgcaggcg agtttttcga
     7141 cgtctccatt gatattcgaa tatgtaccaa cagccccaga aatttttccg acacttattt
     7201 cttttaatgc cgccctcaat ctttcgatat ttcttttggt ttcgaaaaac catccggcga
     7261 ttttaaaccc gaaggtaatc ggttctgcat gaatgccatg tgttcttcca atttgaacca
     7321 ggtctttata atgaagtgcc ttctgtctta gtgtttcaga aaattgttcc aggtcgcgaa
     7381 gcaagattct tgtcgcttta ataagtaata agttggtagc agtatcaaca aggtcagaag
     7441 atgtcatgcc gtaatgaaaa tatctgcttg cttctcctat tgtttcacct atttgttcaa
     7501 caaatgcaat gatgtcatgg ttggtttgtt tttcaatttc ttcaattctt tttatgtcaa
     7561 ttttcgcatg tttttttata tgctgagcaa cagaagaaga aattacacca agttcttgat
     7621 atgcttcaat gacagcaagt tcaacttcca ggtaggttga aaacctggtt tcatctgacc
     7681 agatatctcc aatttctttt ctcgtatatc ttggaatcat acgtatattt tatgttatgg
     7741 ggcaaaaaca gtcaacaata atcctttgcg tttatggtag aattattatc tatgaaaaca
     7801 aaagcaatag gactggtttc tggcggactg gattcaatga ttgcaacgaa attgatgatt
     7861 gaccagggaa tagaggttat cccggttaaa tttacttctc ctttttttca ttcagaagac
     7921 ataaaaaaag aaccgattga ttttctcgga ttgaaattaa gattgataca ggttggggag
     7981 gaatatttgt ctattataag gaatccagag tttggatatg gtgcagggct taatccctgc
     8041 gtggattgca aaatttatat gttgaaagag gcaggaaaaa taatgaaaga aacaggagcg
     8101 tctttcgttt ttaccggtga ggtgttaaat cagagaccgt tttcgcagaa aaaaatattt
     8161 tttgagttgg tgaacaggga aacgttattg aagggaagaa ttttgagacc tctttctgca
     8221 caattgcttg agccgactga acctgagctt gctggcttcg ttgataggtc aaaacttctt
     8281 gcgatatccg gaagacgtag gatgcaacag gtggcgattg cagaaaaaat gggaattaaa
     8341 gattatcctc agcctgctgg aggatgtttg ttaaccgaca aaatatatgc tgagaaactg
     8401 aagaaacttt taaattactg ggatgaatgc actcttaatg atgcggaaat tatcaaacat
     8461 ggaagggtat tctgggcagg agacgtattg ttggtagttg gtagaaataa ggaagagaac
     8521 gaactactta gaactattgc aaagaaaaat gatattatcg tgcaattgaa atctgttccg
     8581 tgtccgctga cagtgttacg gggaaaaaac attgataaca gtgctattga atatgcaaaa
     8641 gatttaattg tgtattatac aaaacgagca ggtcgtgata aaagtgagtt ttcggttgaa
     8701 aaaattcagt aagattcaaa tcagggcaat tacttaattg tgatgtacat tatgaaagag
     8761 aacataagaa attttagttt ggacgaggtt gaacgggtta tttcgtcaat gagcgaacct
     8821 cgttacagag cggctcaggt ttttcgctgg ctttatcaaa aaggtgctga aagtttttct
     8881 gaaatgagtg atgtgccaga aaaattaaga tttcgcctta atcaaaaata tactatagat
     8941 tctttgcaat ccgaccacat gtttgtgtca gaagatggaa caaaaaaatt tatttttaaa
     9001 cttcgcgaca atcgttatat tgaatcagta atgatcactg atgaaaatag aaatacggta
     9061 tgtatttcaa cgcaggttgg ctgtaaatac aattgtgttt tttgtgcaag cggcaagatg
     9121 ggttttataa gaaaccttga tgtttctgaa attattggac agattcttta tattaaatta
     9181 aaagaaaatg taaaaatttc aaatgtagtg tttatgggaa tgggagaacc atttgacaat
     9241 tacgatgcat tgataaaagc aataagtata ataaacgcca gagagggttt gaatatcggt
     9301 gccagaaaaa ttactgtttc tacctctgga attgttcctg gaataaaaaa atttgctgat
     9361 gctgggtggc aggtccgcct ttctgtatca ttgcatgctc ctgacgatgc cacacgcagt
     9421 tcgcttatgc ctgtgaacaa aaagtatcct gttgctgttc tacttgatgc ctgcaactat
     9481 tatatttcca gaacaggaag gagaatcaca tttgaatata ttttgattgc tggtgtcaat
     9541 gacaaagaaa atcatttgtc tggtttggaa agaatagcaa aaaaacttca tgccgatata
     9601 aatgtgatac cgtacagcag aataaaaaac tgtgggttta cttcaccttc tgctgaaaaa
     9661 ataagatggt ttgtgggttc actaaggaaa aaaggtataa aggcgacagt aagaaattca
     9721 aggggcagtg acatcaatgc agcatgtgga caacttgccg gagcaaggag tttctgtgaa
     9781 taaagcaaaa aattattgcg ccattgcgat tgatatagat ggtacaatcg tgcaacctgt
     9841 gagttcctgg agatacattc acgaaaaact tggcaaatgg gatgttctcg cttacagata
     9901 tcaggatatg tttcttgctg gcaagattac ttatcgtcag ttttgcaaac tggacgcagc
     9961 acactggaaa ggtattcctg aaaaaaaaat ttcaagttta ttcaaaaatg ttccatatac
    10021 aagaaatgca aaaagatgtc ttttgaagtt gaaggatatg ggatttcaac taattgcatt
    10081 atccacagga ttacaatata ttcctgagaa gttgcaggat gagattggct tcaaaattgt
    10141 tgtatcaaac agacttcttt cccgcagggg aatattaaca ggcggagtga aaattaatat
    10201 ttctcacggc gagaaaggca ttgttttaag aaaaatcctt aaaaaactta atctatcttc
    10261 agaccaggtt atttgtgttg gagatagcga tggagacata cctatggcaa aaatgtgtgg
    10321 attttcaatt gcatttaatt cttcttcgca gaagttaaac aaagttgttg actttgtctg
    10381 tgaaagtcaa aattttatag aggttttgag cgttatagag aaattaattt ctcagtgatg
    10441 ttactggcga tggtaacctg ccacctcttt tgatgaattt tgatgacgaa aatttgttta
    10501 ctgacatcac aggcatttct ccaagcagtc cgccaaaatt cactttctcc cccgcctttt
    10561 tccctggtac cgggataatt cttgcagcag tggttttgtt gttaataatc ccaatagcaa
    10621 gttcgtcagc gatgattgcg cttatcgtgt ctggcggcgt atctcctgga attgcgatca
    10681 tgtccagacc gacagaacat actgctgtaa atgcttcaag tttttcaatg ttaagggctt
    10741 ttgatgcaac ggcttcagcc atgccagcgt cctcactgac cggaataaat gtgccgctta
    10801 accctcccac atttccgctt gccatggctc ctcccttttt tacagcatcc atcagcaggg
    10861 caattgcaag cgttgaacct ggcgcaccga ttttttctat tccgaacgct tcgataattt
    10921 ctgcaactga gtcacccggt gaaggagtcg gtgcaagcga aatatcaaca attccaaatg
    10981 atactccaat tttctttgcc agttctcgtc caacaagttc tcctgccctc gtgattttaa
    11041 aaactgttct ttttataatt tctgacaatt ctgtcatatc acactcgcga tttttttcta
    11101 caacacttct cacggcgcca ggaccactga ttccaatatt caaagacgct tctgattctc
    11161 cgggtccatg aaatgcgcct gccatgaaag ggttatcttc tggtgcattg acgaaaatca
    11221 caaaccttgc gcaaccaatg ccattatgtg tttttctggc tgttttatta agcatttcgc
    11281 ccacaatttt tacgacatcc atgttcattc ctgaagatgt cgttccagca ttaagaaagc
    11341 aacatacccg ttctgtggaa gcaatgacat caggcataca atcaataagg attttttctg
    11401 cctctgtcat tcctttgtgg acaagcgcac caaaacctcc aatgtagtca atttctgatt
    11461 cttttgcagc cagatcaata atctttgcca tgtcaaggta tttttttgat gttgcgtggg
    11521 tttccataac aagacttatc ggagtgatgg atattctttt gttcacaatg ggaatgccat
    11581 acttgatttc aatttctctt gcgtttttta caagagattt cccgtatctt actatttttt
    11641 tatgtacgtt tttttgaaat cgtgaaaagt ttgagtggat acaatccttg atattgaccc
    11701 caagcgttgt tgttcttata tcaagttttt caaaaagtat cattctcgca gtttcataaa
    11761 tttcttctat tgaaaatggc atgattatat cctgtgcatc attttgaaaa tattctcgtg
    11821 ttgaacctgt atgatcagtc ccatctgttt tccaattttt tcaaggtctt tcacaatggt
    11881 ttccacggaa caatttgcct gcttgatatc aaccagcatt gccataataa aatgatcctc
    11941 tattattttc tgtgtaatgt cttctatatt gatgtttctt ttgaaaagtg tcattgaaat
    12001 tcgcgcaaca ataccttttt tatccttgcc aataaccgtg ataaaaacaa gttctttttt
    12061 cattatatct ccctgagtca ttataacaga aattaggatt ttattccttt ttttcttatt
    12121 attagtttaa ctatttccat catccacaag ggcaaagttg agattgtcat tgctataact
    12181 atatcaattc ccataatagg cactgtcctg aatattgttt ttgataccgg taaataaatt
    12241 gcagccaatt gtaataaaaa tgacaatata acagcataaa caagtttcat gtttgttgaa
    12301 aacttcagtt taaatattga atgaacatcg cttctcagat taaaggaatg aaacaattgc
    12361 gaacaggtaa gcacaaaaaa tgtcatcgtt cttgctcttt caatacctgc tttttctatg
    12421 taaatcacat acaagaatgt ggccagtgcg cacattgcta taaagaatcc ctgcagtatt
    12481 atcatttttc cactttctct attaacaatt tgttcatcgg gttttcttgg cggtttttcc
    12541 atattaccgg ggtctgcagg gtctacaccc agtgccagag caggtagacc atcagtaacg
    12601 atatttatcc agagtatatg aatgggcaaa agtgggaccg gtgttttcaa aaatgaggca
    12661 aaaaacatca gcataatttc tcctgcatta cacgaaagaa ggtaatgcac aaactttttt
    12721 atgttctcat agattgccct gccctcttcc actgctgcca caatggatgc aaaattgtca
    12781 tcggttatga tcatgtcaga cacttctttt gtcacatctg ttcctgtgat tcccattgca
    12841 attcctatgt ctgcctcttt tatagcagga gcatcattaa caccatctcc tgtcattgcg
    12901 acaatttcat tgttttctct taatgctctg acaattctga gtttatgatg gggggataca
    12961 cgggcaaaaa cagaaacttt gttcactgtt tcctgtaaat gattatcgtc taaactatca
    13021 atccggtctc ctgctatcac tgcatgttca ttttgaatta atgatagttc ctttgctata
    13081 gcaattgctg ataatttgtg gtctcctgta atcattttga ctttgatacc ggctttctta
    13141 catttttcga tagcaagttt tgcttcatcc cttggcgggt caatcatggc aaaaaggcct
    13201 gtgaagacaa tgtttgtttc aaccgttttt tccgaaatat ctgcatcggg ttcaatattt
    13261 ttatatccag tggcaagaac cctcaatgct tgtttagcaa aactgtcagt tatatcaaga
    13321 attaattttt tccctgtttc atcaagatgc tttatggaat tattctcttc aataaatgag
    13381 cacagattca gtataatgtc aggtgctcct tttataaagg atacgagatt ctgattatgc
    13441 ctgacaatta ctgtcattct ttttctttct gaatcaaaag gaatttcatc gacgatctgg
    13501 aatgtatttt ctatttcttc tttcaatttg cctgcttttg ccgcaacagt taaaagggca
    13561 ccttctgtgg ggtccccggt cactatccac atattgttat gttttgtcag ccgcgcacca
    13621 ttgcatacaa cagcacacag gagtgacctg ttaagtcctg gaaattgttc gggtaaaaca
    13681 ggtctatcat tgatcaaaaa attaccctgt gggtcatagc cagcgccagt gacagagaaa
    13741 gttgttccgt ctgcataaat ttttttgacc gtcatttcat ttttagtaag cgtgccggtt
    13801 ttatcggaac atatcgtggt tgcacagcca agcgtttcaa cagaaggaag ttttctgata
    13861 agcacatttt tctttgccat tcgttgaaca ccaagggcaa gaccaattgt taccaccgca
    13921 gga
//
LOCUS       DAORYU010000008        13753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_3521_length_13753_cov_4.354139, whole genome shotgun sequence.
ACCESSION   DAORYU010000008 DAORYU010000000
VERSION     DAORYU010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13753
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3521_length_13753_cov_4.354139"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..712
                     /locus_tag="PLW07_00645"
     CDS             173..712
                     /locus_tag="PLW07_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49065.1"
                     /translation="MGILNEDERARVIEEEEIRVSVRRKYEQKSSGVAAVLSTLCPGL
                     GQVYNGQFGKGALFFITVVVGLVLFVWGLVATIKEVRAFAGGHQMSTVSEGTGISSEK
                     PVPLTEEGIVVEEVEKEEKAQTEGKQEKQEESITKYIPKKYVAFTIIGIILMVGGAGL
                     AVKDAIKIAKRINQGIVKG"
     gene            complement(716..1480)
                     /locus_tag="PLW07_00650"
     CDS             complement(716..1480)
                     /locus_tag="PLW07_00650"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HOL49066.1"
                     /translation="MLENKLAKLIREKKPSLGGWINLTDPGIAIIMARAGYDWLLIDN
                     EHHPFTETQIQAMIYALRTTGVTSIIRVRGNDPSHIKWVLDTGAGGIMVPMLENAQDV
                     KKAISYSKYFPLGNRGYSPLRATDFWAFKQEYEEKANREVLLICQIEQPSAVNEIEEI
                     VKLPVDGVWIGYADLSYSMGYHGNFKHPEVQKAIDKVIDAANKYNKPWGMPAATPEDF
                     ALRVKQGALLMIAGSDVYFLNSGATERVRQCRKAIE"
     gene            1558..2571
                     /locus_tag="PLW07_00655"
     CDS             1558..2571
                     /locus_tag="PLW07_00655"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOL49067.1"
                     /translation="MRPKNDRKWIELIINHREMPVPYNFMFSPVSLKIAEQHYGSPIA
                     DAINLPIRMISPKSIKPLYADPAKFGDTAVDEYGVRWSTSAIDRGAPTGPVLKNPDLS
                     GYRFPDPGLPYRFEDIENWCKKQEDHYRIIWVGDLWERATFMRGMENLLMDIVLHPGF
                     VMELLSGITDYILGTMEILFERFEFEAIALSDDYGTQKGMIISPEMWGKFIRPFLAKI
                     YGFAAKNGKKIFQHSCGYIIPIIPDLIELGLDILHPVQPEAMDIYYLKKEFGTDLCFC
                     GGISTQDLLVRGTPEQVRNEVKRLKETMGKGGGYILEPGITIQADVLAHNLFAMIDEA
                     KNI"
     gene            2574..3233
                     /locus_tag="PLW07_00660"
     CDS             2574..3233
                     /locus_tag="PLW07_00660"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOL49068.1"
                     /translation="MALAEKKMYLANLVNLLKKQWPENRTVNIVCHGHSVPAGYFATP
                     FVDTFNAYPHILHRKLKERFPLAVINVIVTAIGGENSISGAKRFSQDVLCHKPDLVLI
                     DYGLNDRTADASSVQNAWTSMVRQAKENNVAVMLLTPTWDFSVVDPQQDYGKLLKKQA
                     EIIRTIASEEEVAFADSYTAFEKAVSNGVSLETLMSWINHPSRKGHEIVVDEILAWFP
                     F"
     gene            3254..4897
                     /locus_tag="PLW07_00665"
     CDS             3254..4897
                     /locus_tag="PLW07_00665"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HOL49069.1"
                     /translation="MIFKGKKNLPSWRIEFPEPAIKNRFFHIDLKGSKIPFDIFCGLL
                     EQLARWGINGVVVEYEHRIPYLPLPKQFPEKERYTENEITRLLKHAQSLGIQWIPLIQ
                     TFGHVEYLSRLQGTKNLFENPEYPSQLCPSKKIVKDYLEKLIDYVCTLHAESQYIHVG
                     QDETRQLGMCKRCSERMKESGGKIELYLEHVQSVWEQVFKHNKIPMAWADMFIGNGRL
                     DLVQKIDRRVILIPWDYTSAGNRSKFVIYKGYRPTRKQFYNEYIPEPEQPAAFVKKGQ
                     FFEDLEPEEIRRIGIDESTNYPASFAQLKLMTTTGVPLWGACGIYMSADMPIRANYVR
                     GLLNPAHMCDFLIKNQGEGIIGTLWARGHSFAPINAMWTLTFYNIVQFARASWTGKVT
                     PDDFRKSAEEIAFELDMPSTFDEHWTLDDILRMISPPKVPNFDIVYNIVEKGNVTGCF
                     GDGLNLSLKAECLRNKIRSIIEEARWWYSTRKEMPSPLKKDMKNRFFSALKEMRVLKK
                     PMKEYYLKWVGDKDSFNLWWDNLFTLDIILARSVLEKFL"
     gene            complement(4899..5576)
                     /locus_tag="PLW07_00670"
     CDS             complement(4899..5576)
                     /locus_tag="PLW07_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOL49070.1"
                     /translation="MKRGFTLVELLVVIAIISMLAGQIMPSLSTAREKGRQANCINNL
                     HQIGIAMEIYYQDYDDYPTWLSIMVPSYLATDKIFICLTDRYRGVTGHGNPQYPGSND
                     IPPAQIPGYSPPYEPEFAYRNPNVTACSYIYEFSPCACEWFMQGYASGTYTDTDLNQA
                     DTNRDGKVSWKEAKLWQASTQGLSDNLPIVRCFWHNYNYGQKVLNLAYKDYHVFTSRM
                     YWENSAD"
     gene            complement(5573..6277)
                     /locus_tag="PLW07_00675"
     CDS             complement(5573..6277)
                     /locus_tag="PLW07_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOL49071.1"
                     /translation="MKKIKVFYRGLSQKNRIIFWVCFAVILLSLFAIGRTIYRATTSK
                     NPTIENYMKQIRSKDPAQRETGVYTVGLYRVKEMADTLENMIRQDPEIKVKKVAAWSL
                     GRIDINRLVKLLDSQDTDIKDIAMEALIKLDKNNVSYMMERFAKEDVETKKKILGVID
                     TLKKPDFNGSLIEVAENKDENAEIRLQALSILKDTGTMELEGRLNNIYYNDPATEMKE
                     AAKHTLEFIKNREKNK"
     gene            6654..9263
                     /locus_tag="PLW07_00680"
     CDS             6654..9263
                     /locus_tag="PLW07_00680"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HOL49072.1"
                     /translation="MKGRRKVCRKASRMVVLLSGLRMSGFFLAALIAICAGFISTGNA
                     AISFSSSVQYPASGNYGEPFAITSGDFNNDGYPDIAFVDNKDNYLYVLLNNGDGTFNS
                     TPSARIKVDKKPQGIACGDLNNDGKLDIVVSRYDADNVRVYLGNGDGTFTYKNEFATE
                     NQPVGVVLAHLNSDNYLDLIVCNYGNKSFSVLTGKGDGTFNTKVKYSTNNDNPWAAAV
                     GDFDGDEDMDVVVTHVGKDKIASVFKNNGSGDFGSQLDVAVGNKPNGVVTGDFDGDED
                     IDFATGDHDGKSVTVCKNNGTGTSFTPTTYTDSSLEHVRGIVAADFDGDEDLDLAVTN
                     DHDNKVIILENNGSGSFSFVGTGFPVGSEYPRGITAADFDKNDMPDLATANTDGHNIS
                     VLINSGTPPAPTTYSISGTVSLIGGGNVQDVLLTLSGASSQTTNPDASGFYSFTGLAS
                     GNYTVTPSLADYEFTPDSRNYNPLSSDQTYQDFLGVRQCKLTINSDYDTPVPDAGDHI
                     YDYDDVVSCSVDPEVVDGTTKYICTGYTLDGNTVNGQTSVDVTMDGEHVLTWNWKTQY
                     YLTVNSVRGNPQGEDWYDAGTTANWSVTSPVVDGGTRYVADQASGSVKMDEPKTADVN
                     WSTQYYLTTVADPIAGGSVSPTSGWFDAGSNVQIQAFPNQGWKFSNWSGDASGSQNPA
                     SITMNGPKTARAVFAPLTQYTISGKVYRANGTVFPGVTVEYTGVWNGTSVSGSTTTAT
                     DGSYSLLLPGGWTGTVAPKNSSSMFTPANRYYASLSSNQTNQNYNALGGYAISGYVKT
                     STGQPISSVILNFGGYGTAITNSTGYYTKTVTAGWSGTVTPYRSGYKFTPASRTYSNV
                     LKNYANQNYTGSR"
     gene            complement(9330..10358)
                     /locus_tag="PLW07_00685"
     CDS             complement(9330..10358)
                     /locus_tag="PLW07_00685"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOL49073.1"
                     /translation="MGKNFLQIVLFGDSMTWSPGVAIGKRYADFIQEKLQNICGDNYI
                     VDVAACGDGGNTAEEAYARIERDCISYQPHIVVISLGANDCIRAPDRETFKSYYRKIV
                     NEVKHDATRHIILETIPALDEELHSQRNNPKALFYGGLEKYVEFFSHSFIREMAKTGD
                     FLLYDRFTLYHQMLAENPQLREKLILKDGVHLTEQGNEFFAENLVPIIQPLIPDIHQF
                     QTNAEQYLEQALLNPVYIECTQSLKNGNLKEYLTEHPNLKRLMLEKTKSTSRRAIAYT
                     EDEKVKNEATIVQYLTSGFLAAEKIFNSQDSEIIGKSKQWAISHLAKLSNNRVAKTLV
                     ELMNETCL"
     gene            complement(10362..13385)
                     /locus_tag="PLW07_00690"
     CDS             complement(10362..13385)
                     /locus_tag="PLW07_00690"
                     /inference="COORDINATES: protein motif:HMM:NF020121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HOL49074.1"
                     /translation="MRKVFFILFVMSVSLYAQSNNILFKQEVFPLTNPGFEQLSAGWN
                     IKPTEKTQPLPDVVFSFENVACEGNYSFAITLNKRTSTYIPRSFQVEKGKKYYMSAKI
                     KTVGQISGTFRVVYGATGEPYANRVGPDSDWTIAGLTFVGGRHSRGFGEPKNPDPNIS
                     YCELRLEANGIGTVYFDDIKIYEMKEYGEYVRFKLIQPENKSYRVKIHGLYGPPSWFF
                     TITPKETFSSGTTSTWIDLGTYDQLKGRGVVYAGFHFEPISGEKFETIEATIDLAYRP
                     EDSCIIKSFTRTTPGNIIGVIIPKTESNPDDFLKGFALLDQDIIKRNQFVKSLNLPPV
                     NLKKFYLEAHLKGFGSMFSDPVMAETEIDTIRTMGFNALDTQYSGLAGVYRQVAEKYG
                     ITQTHHTFRIGRLPQDPVSKRVIFDLSKIRQTCAEFVEKTIENLKKQDPDQISIIKFI
                     DTGDEIAGEVFGGIEYEQGYKEYVKSQGLKPSDLKAKNWDEVKIYGSWGWRDSWKMRP
                     TDRTAVDSCINYYWTLRYWNYATAKVYTILAEEITKRMPSVKVRVNFGPPWAYGYCSY
                     MRGAEIWEFARQNSVTDFWNEDWLNTSGWRNAGIQMVSYLVDLSKSCARLNNADVSAF
                     VMPVAKPENIQLKLASVIGKGAKKIDVYRYGPAYASPDNWSGSTDMAQGISHFTRILE
                     KAEGILFSGKPRKPELAIIWSAGEPVWATDDASMWNQQLIYLALQHQQIPVDFVDEFM
                     VEQGALKQYKVAVLTSQYLRKSTKKAIVDWVSNGGNLWVDGIPATNDEYGQPCEMLLP
                     VIGIKDITVVDSAIGRSMNPQNGVNLSIIPGTIKMTQSDDTISAVGRKIIFSLRNPEK
                     TKVIGVWEDNKPAVIEHRYGKGRVFYAGTYAGHSYNSGVERIPGKIEIGYREKERKLI
                     TDFILGCGIQRPVWCSVACVQADLLESDQGIGIVLSNYSGIPQNQITVYIDAKKPVSS
                     VVSAQKGLLQFTQDTKTNIITTSVSLDIFDFILLK"
     gene            complement(13411..>13753)
                     /locus_tag="PLW07_00695"
     CDS             complement(13411..>13753)
                     /locus_tag="PLW07_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49075.1"
                     /translation="NGKTWTNPVKTNFPNATSKFFGLITSRKFRVFVSNANRNMNILR
                     RQMHLSVSTDGRVFTGIARIDIPTDGKATLQYPHAMEYKGQLLIAFSRNKKIIELITI
                     PLERIEKLFTL"
BASE COUNT         3960 a         2718 c         2881 g         4194 t
ORIGIN      
        1 aatcgttcat aaagacatcc attttctttc ctggagagaa agtggagaaa aaacctaaaa
       61 caagtggaga gggatatctt tattcacttg ggtgctgcgg cacagacaaa actacggata
      121 aatccgcatc ccctgcaaac ttgccttttc aaaacctgaa aaaggagggt ttatgggtat
      181 attgaatgaa gatgaaagag caagggttat tgaagaggaa gaaataaggg tatctgtgag
      241 aagaaagtat gagcagaaat cttcaggtgt tgcagcagtg ctttcaactc tgtgtcctgg
      301 tctcggtcag gtctacaatg gccagtttgg aaaaggtgca ttgtttttta tcactgttgt
      361 tgtcgggctg gttctttttg tatgggggct tgtggcaaca atcaaagagg taagggcttt
      421 tgcaggtggg caccagatga gcacggtttc tgagggtact ggcatttcgt cagaaaaacc
      481 cgtaccactt acagaagaag gaattgttgt tgaagaagtt gaaaaagagg aaaaagctca
      541 gacagaagga aaacaggaaa agcaagaaga gtcaatcaca aaatatatac caaaaaaata
      601 tgtggcattc acaataattg gaatcattct gatggtgggt ggcgcaggat tggctgtaaa
      661 ggatgccata aaaattgcaa agagaatcaa tcaggggatc gtaaaaggat aattttcatt
      721 caatcgcctt gcgacattgt ctgactcttt cggttgcacc tgaattaagg aaatagacat
      781 cagagccagc tatcatgagt aatgctccct gttttactct cagggcaaaa tcttctgggg
      841 ttgcagctgg catgccccag ggtttgttgt atttattggc tgcatcaata accttatcaa
      901 ttgccttctg gacttcaggg tgtttgaaat tcccatgata gcccattgaa tacgaaaggt
      961 ctgcataacc aatccagacg ccgtcaaccg gtagtttcac aatttcttct atttcattga
     1021 cggcagaggg ctgctctatc tgacagatta aaagtacttc cctgtttgct ttttcttcat
     1081 actcttgctt gaatgcccag aagtcagttg cccgcaaggg actgtatccc cggttcccaa
     1141 gcgggaaata ttttgaataa gagattgcct tttttacatc ctgggcgttt tccaacattg
     1201 gtaccataat tccgcctgca ccagtatcaa gcacccattt gatatgggat ggatcgtttc
     1261 ccctgaccct gataatggaa gtaacaccag tggttcttaa agcgtaaatc attgcctgta
     1321 tctgtgtttc agtgaaagga tggtgttcat tgtctatcaa aagccagtca tatcctgcgc
     1381 gtgccatgat gatggcaatc ccagggtcag tcagattaat ccatccacca agcgatggtt
     1441 ttttctcgcg aataagtttt gcaagtttat tttccagcat ggcaatgtta tattatcaca
     1501 attggctgga tttacaagat ttgaaaaaag aaatttttgt gataatttaa ttggcatatg
     1561 agaccaaaaa atgacagaaa atggatagaa ttaataatca atcacaggga aatgccggta
     1621 ccatacaatt ttatgttttc acctgtttct ttgaaaattg ctgaacaaca ttatggttca
     1681 ccgattgctg atgcaatcaa tcttcccatc agaatgattt ctccaaaatc cattaaaccc
     1741 ctttatgcag accctgcaaa gttcggcgac actgcagttg atgaatatgg cgtgagatgg
     1801 tcaacaagtg caattgacag gggcgcgcca acaggacctg tattaaaaaa tccagacctt
     1861 tctggataca ggtttccaga ccctggtttg ccgtacagat ttgaagatat tgaaaactgg
     1921 tgcaaaaaac aagaagatca ttacagaatc atatgggttg gggatttatg ggaaagagca
     1981 acatttatga gggggatgga aaacttgctt atggatattg tgttacatcc aggttttgtt
     2041 atggagttgt tatcgggtat tactgactac attctcggga caatggaaat cctttttgaa
     2101 agatttgagt tcgaagccat tgcactcagt gatgactatg gaacgcaaaa agggatgatt
     2161 atttctcctg aaatgtgggg gaaatttatc aggccatttc ttgcaaaaat atatggattt
     2221 gctgcaaaaa atggtaaaaa aatttttcaa cattcctgtg gatacattat tccgattatt
     2281 cctgatttga ttgaactcgg gcttgacatt cttcatccag tacagccaga agcaatggat
     2341 atttattatc tgaaaaagga atttggaact gatctgtgtt tttgcggcgg tataagtacg
     2401 caggatttac tcgtgagagg tacgcctgag caggtgcgaa acgaggtaaa gcgactgaaa
     2461 gaaacaatgg gaaaaggcgg tggttatatt cttgaaccag gtattaccat tcaggcagat
     2521 gttcttgctc acaatctgtt tgcgatgata gacgaagcaa aaaacatata aaaatggcat
     2581 tggcagaaaa aaagatgtat cttgcaaatc tggtgaatct gttaaagaaa cagtggcctg
     2641 agaacaggac tgtgaacatt gtctgtcacg gtcacagtgt gcctgctgga tattttgcca
     2701 caccgtttgt tgacacattc aatgcctatc ctcatattct ccacaggaaa ctgaaggaaa
     2761 gatttcctct ggctgttatc aatgtgattg tgactgccat tggtggagaa aattcaattt
     2821 caggcgcaaa aaggttttca caggatgtgt tgtgtcacaa gccagacctc gtactgatag
     2881 attatggact caatgacagg acggctgatg ccagttctgt tcaaaacgca tggacatcta
     2941 tggtaaggca ggcaaaggag aacaatgttg cagtaatgct tttaactccg acgtgggatt
     3001 tttctgttgt tgacccacag caggattatg gaaaacttct taaaaagcag gcagaaatta
     3061 tcagaaccat tgcatccgaa gaagaagttg cctttgctga tagttacact gcttttgaaa
     3121 aggcagtgtc aaatggagtt tctcttgaaa cattgatgag ctggattaat catccttcaa
     3181 gaaaagggca cgaaattgtt gttgatgaaa tacttgcctg gtttccattc taattctaac
     3241 aaaggaaaac gaaatgattt tcaagggaaa aaagaatctt ccttcctgga ggattgaatt
     3301 tccagagccc gcaataaaaa atagattttt tcacattgac ctgaaaggat caaaaatacc
     3361 atttgatatt ttctgcgggc ttcttgagca acttgcaaga tggggaatta atggggttgt
     3421 agttgaatac gaacacagga taccatattt gcctttacca aaacagtttc cagaaaaaga
     3481 aagatacaca gaaaacgaga taacaagatt gttaaagcac gcacaatccc ttggtattca
     3541 gtggataccc ctgattcaga catttggaca tgttgaatat ctttcaagat tacagggaac
     3601 aaaaaaccta tttgaaaacc ctgaatatcc atcacagttg tgtccgtcaa aaaaaatcgt
     3661 gaaagattat cttgagaaac tcattgacta tgtttgtacg ctccatgcag aatcacagta
     3721 tattcatgtt ggacaggacg aaacaagaca attgggtatg tgtaaaaggt gttctgaaag
     3781 aatgaaggaa tccggtggaa agattgaact ctatcttgag catgtgcaat ctgtctggga
     3841 acaggttttc aagcacaaca aaataccgat ggcatgggca gatatgttta ttggtaatgg
     3901 acggcttgat cttgttcaaa aaatagatag acgggtcatt ttaattccat gggactatac
     3961 ctctgcagga aacaggtcaa aatttgtgat ttacaagggg tacaggccca cgagaaaaca
     4021 attttacaat gaatacatac cagaaccaga acaaccagca gcatttgtta aaaagggaca
     4081 gttttttgaa gaccttgagc cagaggaaat aagaagaatt ggaatagatg aaagcacaaa
     4141 ttatccagca agttttgccc agctgaaact gatgacgaca accggtgtac cgttgtgggg
     4201 agcatgcggt atttacatga gtgctgatat gccgattcgt gcaaactatg tcaggggtct
     4261 attaaatcct gcccatatgt gtgattttct tatcaaaaat cagggagagg gaattattgg
     4321 gacactatgg gcaaggggtc attcatttgc tccaatcaat gcgatgtgga cccttacctt
     4381 ttataacatt gtccagtttg caagggcttc gtggacaggt aaggtaacgc ctgatgattt
     4441 cagaaaaagt gcagaagaaa ttgcgtttga acttgacatg ccttcaacat tcgatgaaca
     4501 ctggacactc gacgacatat tgaggatgat ttctccgcca aaggttccga attttgacat
     4561 tgtttataat attgtggaaa aagggaatgt cacaggttgt tttggtgatg gcttgaatct
     4621 ttccctaaag gcagagtgtc tgcgaaacaa aatacggtca atcatagaag aggccaggtg
     4681 gtggtactca acaagaaaag aaatgccatc ccctttgaaa aaagatatga aaaacagatt
     4741 tttttctgcg ctcaaagaaa tgagagtact gaaaaaaccg atgaaggagt attatttgaa
     4801 gtgggtgggc gataaagatt ctttcaatct gtggtgggat aatcttttta cccttgatat
     4861 catacttgcc agaagtgtgt tagaaaaatt cctgtaaatc agtcggcaga attttcccag
     4921 tacatcctgc ttgtaaatac gtgatagtcc ttataggcaa ggttcagaac cttctgtcca
     4981 taattgtaat tatgccagaa acatcgcaca attggcaaat tgtcagaaag tccctgagta
     5041 cttgcctgcc agagttttgc ttctttccat gacacctttc catccctatt ggtatctgcc
     5101 tgattcaagt cagtatcagt atatgttcca cttgcgtatc cctgcataaa ccactcacag
     5161 gcacatgggc tgaattcata aatgtaactg catgccgtca catttggatt tctatatgca
     5221 aattcaggtt catagggtgg actgtagcca ggtatctggg ctggtggaat gtcattgctt
     5281 ccaggatatt gaggattgcc atgacctgtg acacccctgt atctgtctgt caggcatata
     5341 aaaattttgt ctgttgcgag ataggatgga accatgattg aaagccatgt gggataatca
     5401 tcatagtcct gataataaat ttccatggca atcccgatct gatgcaagtt attgatacaa
     5461 ttcgcctgtc ttcctttttc tctggcagtt gacagcgatg gcataatctg accggcaagc
     5521 atactgataa tcgcaatcac cacaagaagt tcaacaagag tgaaacctcg tttcatttgt
     5581 ttttctccct gtttttaatg aattcaaggg tatgtttggc tgcctctttc atttcagttg
     5641 ccgggtcatt gtaatagata ttattcaatc tcccttcaag ttccattgtt cctgtatctt
     5701 taagaatact gagcgcctga agtctgattt ctgcattttc atctttgttt tcagcaactt
     5761 ctatcaggga tccattaaaa tccggttttt tcagggtatc aataacaccc agaatttttt
     5821 tctttgtttc aacatcttct tttgcaaatc gttccatcat atatgagacg ttatttttgt
     5881 ctaatttgat tagtgcttcc attgcaatat ccttaatgtc tgtgtcctgt gaatcaagca
     5941 attttaccaa tctattgata tcaatccttc ccagtgacca ggcagcaacc ttttttacct
     6001 tgatttccgg gtcctgtcgt atcatatttt ccagtgtatc agccatttcc ttaaccctat
     6061 aaagtcccac agtatataca ccagtttctc tttgagcagg gtccttactg cgaatctgtt
     6121 tcatataatt ttctattgtg ggattcttgc tggtggttgc tctataaatc gttctgccta
     6181 tggcgaaaag ggaaagcaaa attactgcaa agcagaccca gaagataatt ctgtttttct
     6241 gggagagccc cctgtaaaaa acctttatct ttttcatagt cctattgtac cgagtgattt
     6301 ttattctgtc aatagatgtt aagttttcaa tttttctgtt aatgggaaac ctgttctttt
     6361 cacgacactt gacaggataa aataatatgg tattctgata atgaaagcag ttgagtagaa
     6421 gttaacaatg gttgcacttc cgaagacaat tatggaagta taaagccatc cttaagagcc
     6481 ttcctgagtg ttacccgccg gagacaatac gcaagcaaga agtttgtcag aaccagatcg
     6541 atcggcaaag aggtatcaaa aaaatttctc agacacaggg acaggcatgt gttctatttt
     6601 gaaatcctga tagattaatc tcatgccatt attctgtgtt aaaggaggca gatatgaaag
     6661 gtagaagaaa ggtgtgtagg aaggcaagca ggatggttgt tttactgtca gggctccgga
     6721 tgtctggttt ctttctggct gcactgattg caatatgtgc tggttttatc tccacaggaa
     6781 atgcggcgat ttcattttcc agtagcgtgc aatatcctgc ttctggtaat tatggagaac
     6841 cttttgccat cacttctggt gatttcaaca atgacggata tcctgacatt gcctttgtgg
     6901 acaataaaga caattatctt tatgtattgc tgaataacgg cgacggtacc ttcaattcca
     6961 cgccatctgc caggatcaag gttgataaga agccacaggg tattgcctgt ggtgacctga
     7021 acaacgatgg caaacttgac attgtggttt ccaggtatga tgctgacaat gtgcgggttt
     7081 atctgggcaa tggagatgga acctttactt ataagaatga atttgccacc gagaaccagc
     7141 cggttggagt ggtacttgcc cacctgaact ctgataacta ccttgacctg attgtttgca
     7201 actatggaaa caagagtttt tctgttctta ccggcaaggg tgatggcacg ttcaacacta
     7261 aggtaaagta ctctacgaat aacgacaatc cctgggcagc cgcagtcggt gattttgacg
     7321 gtgatgagga tatggacgtt gttgtcactc atgtcgggaa agacaagatt gcctctgttt
     7381 tcaagaacaa cggttcgggt gatttcggca gccagcttga tgtagccgta ggaaacaaac
     7441 caaacggtgt ggtgaccggt gattttgacg gtgatgaaga tattgatttt gctactggtg
     7501 accatgatgg aaagagcgtc actgtgtgta agaacaatgg cacagggaca tctttcaccc
     7561 cgaccaccta cactgatagt agtttggaac atgttcgcgg tattgtggca gcggattttg
     7621 acggtgatga agaccttgat ctggcagtca ccaatgacca tgacaataaa gtaattattc
     7681 ttgagaacaa tggaagtggc agtttttcgt ttgtgggtac tggtttccct gtgggcagcg
     7741 agtatcccag gggaatcact gcggctgact ttgacaaaaa tgacatgcct gatttagcca
     7801 ctgccaacac cgatggtcac aacatttctg ttctcatcaa ctctggcaca ccgccagcac
     7861 cgaccaccta ttccatctca gggacagtgt ctttaattgg tggtggaaat gtgcaggatg
     7921 ttcttttaac tctttcaggc gcgtcttcac aaacaacaaa tcctgatgct tctggatttt
     7981 actcgttcac aggtcttgct tctggaaatt atacagtaac cccatctctg gcagactatg
     8041 aatttacgcc agattcacgc aattacaatc cattgagttc agaccagacc taccaggatt
     8101 ttcttggagt aaggcagtgc aaactcacga taaattctga ttatgacacg cctgtacccg
     8161 atgctggaga ccatatttat gattatgacg atgttgtaag ttgttcagta gaccctgagg
     8221 ttgtagatgg tacaacaaaa tacatctgca ccggctacac gctggatggc aacacagtga
     8281 acgggcagac atcagttgat gtcacaatgg acggtgaaca tgttttaacc tggaactgga
     8341 agacccagta ttatctgaca gtgaattcag tgcgcggcaa tccgcagggt gaggactggt
     8401 atgatgcagg aacaacagca aactggtcag tgaccagtcc tgtggtggac ggtgggacaa
     8461 ggtatgttgc agaccaggca tctggctcag tcaagatgga tgaacccaaa actgcggatg
     8521 tgaactggtc aacccagtat tatctgacaa cagttgctga cccaattgct ggcggtagcg
     8581 taagtccgac cagcggctgg tttgatgctg gcagcaatgt ccagatacag gcatttccca
     8641 accagggctg gaagttttct aactggtctg gcgatgcaag tggcagtcaa aatcctgcca
     8701 gtatcaccat gaacgggcca aagacagcaa gggcagtttt tgccccgctt acccagtaca
     8761 cgatttcagg taaggtttat cgcgccaatg gtacggtttt ccctggtgtg actgttgagt
     8821 atactggcgt ctggaacgga acgagtgtca gtggttctac aacgacagca acagatggta
     8881 gttacagttt gttgcttcct ggaggttgga caggtacggt tgctcccaag aattcttctt
     8941 ccatgtttac tccggcaaac aggtattatg cttctctcag cagcaaccag acaaaccaga
     9001 attataatgc tctcggtggt tacgcaattt ctggttatgt gaaaacaagt acaggtcagc
     9061 ccatatccag cgtgatttta aattttggtg gctacggaac agcaatcacc aattccacag
     9121 gatattatac aaaaacagtg actgctgggt ggagtggtac tgttactcct tacagatcag
     9181 gttataaatt cacacctgca tcaagaactt attccaatgt cttgaagaat tatgcgaacc
     9241 agaattatac aggaagcagg taataatcag tagtgttatg cggaaataaa aagccccgca
     9301 aaaaaaagcg gggctttttt attgctactt cacaggcatg tttcattcat aagttcaaca
     9361 agtgtttttg ctactctgtt gttagatagt tttgcaagat gggaaattgc ccattgtttg
     9421 ctttttccta ttatttcact atcctgcgaa ttgaaaattt tttcagcagc cagaaatcct
     9481 gatgtaaggt attgaacgat tgttgcttca ttttttacct tttcatcttc tgtatatgca
     9541 attgccctgc gactggtgga ttttgttttt tcaagcatca atcgtttgag attcggatgt
     9601 tcagtaagat attcctttag attgccattt ttcaatgact gggtacattc aatataaacc
     9661 ggattaagaa gtgcctgttc aaggtattgt tctgcgtttg tttgaaattg atgaatatcg
     9721 ggaatcaatg gttgaatgat tggcacaagg ttttcagcaa aaaactcatt tccctgttct
     9781 gtaagatgaa cgccatcttt taaaatcagt ttttctcgca actgtggatt ttctgcaagc
     9841 atctgatgat acagggtaaa tctgtcatac aagagaaaat ctccagtttt tgccatttcc
     9901 ctgatgaatg agtggctgaa aaattcaaca tatttttcaa gtcctccata aaacagggct
     9961 ttcgggttgt ttctctggct gtgcaattct tcatcaagag cagggattgt ttcaagaatg
    10021 atgtgtctgg tcgcatcgtg tttaacctca ttcacaattt ttctgtaata tgatttaaac
    10081 gtttctctat caggtgccct gatacaatca tttgcgccca aactgattac cacgatatgc
    10141 ggttgataag aaatgcaatc ccgttcaatt cgtgcatatg cttcttctgc tgtgttgcca
    10201 ccatctccac atgcagcaac atcaacaatg taattatcac cgcatatgtt ctgaagtttt
    10261 tcttgaatga aatctgcata tcttttgcca atggctactc ctggtgacca tgtcattgaa
    10321 tcaccaaaca atacaatttg aagaaaattt tttcccatgt tttatttcag caaaatgaaa
    10381 tcaaaaatat caagtgacac actggttgtt atgatgtttg tttttgtatc ctgtgtaaat
    10441 tgcaatagtc ctttttgagc agacacaaca gatgagacag gttttttagc gtcaatatac
    10501 acagtaatct ggttttgagg tattcctgag tagtttgaaa ggacaatgcc aataccctga
    10561 tcgctttcaa gtaagtcagc ctgaacacaa gccactgaac accatacagg tctttgaata
    10621 ccgcaaccaa ggataaaatc agtaatcagt tttctttctt tttctctgta tccgatttct
    10681 atttttccag gtattctttc aacaccgcta ttatatgagt ggccagcata tgtgccagca
    10741 taaaataccc tgccttttcc ataccgatgc tcaatcacag caggtttatt atcttcccat
    10801 acaccaataa cttttgtttt ttctggattt ctcagagaaa aaataatttt tcttccaact
    10861 gctgaaattg tgtcatccga ctgcgtcatt tttattgttc caggaatgat agataaattg
    10921 actccatttt gtggattcat ggaccttccg attgcgctat caacgaccgt gatgtccttg
    10981 attccaataa caggcaaaag catctcacac ggctgaccgt attcgtcgtt tgttgctggg
    11041 ataccgtcaa cccacaggtt accaccattt gatacccagt caacaattgc tttttttgta
    11101 gattttctta agtattgact ggttaacact gcaaccttat attgttttaa tgctccctgt
    11161 tcaaccataa attcatcaac aaaatccaca ggaatttgtt gatgctgtaa agcaagataa
    11221 atcaactgct gattccacat actggcatca tcagttgccc acacaggttc acctgcagac
    11281 catataatgg caagttctgg tttcctcggt tttccagaaa aaaggatacc ttctgctttt
    11341 tcaagaattc ttgtaaaatg agaaatgccc tgtgccatat cagtgctgcc agaccagtta
    11401 tcaggagaag cataggcagg accatacctg tacacatcaa ttttctttgc gccctttcca
    11461 atgacagaag caagttttaa ctgaatattt tcaggttttg caacaggcat gacaaatgcg
    11521 ctgacatctg cattatttaa tctggcacaa ctcttgctca aatcaacaag atatgaaacc
    11581 atctgaattc cggcatttct ccatccagat gtattcaacc agtcctcatt ccagaaatca
    11641 gttacactgt tttgtcttgc aaattcccat atttctgctc cacgcatata agaacaatat
    11701 ccatatgccc agggtggacc aaaattcacc cttactttca cagaaggcat tctttttgtg
    11761 atttcttctg caagaatagt gtaaactttt gctgtagcat aattccagta gcgaagtgtc
    11821 cagtaatagt tgatgcagga gtcaacagca gttctgtcag tgggtctcat tttccaggaa
    11881 tctctccatc cccatgaacc atatattttt acttcatccc agttttttgc ttttaagtcc
    11941 gatggtttca gtccctgtga ttttacatat tccttatatc cctgttcata ttctattcct
    12001 ccaaaaactt cgcctgcaat ttcatctcca gtatcaataa atttgatgat tgatatctgg
    12061 tcaggatcct gttttttcaa attttcgatt gtcttttcca caaactctgc gcatgtctgc
    12121 ctgatttttg ataggtcaaa aatgacacgt ttactgacag ggtcctgggg taatcttcct
    12181 attctgaagg tgtggtgtgt ttgtgtaatg ccgtattttt ctgcaacctg tctgtaaaca
    12241 ccggcaagtc cagaatattg tgtatcaagc gcattaaaac ccattgttcg tattgtgtca
    12301 atttctgttt ccgccatcac agggtcagaa aacattgacc cgaatccttt aaggtgtgcc
    12361 tcaagataaa atttcttcag gttcacaggc ggtaaattca atgattttac aaactgattt
    12421 ctcttaatga tatcctggtc aagtagcgca aatcctttca aaaagtcatc agggttactt
    12481 tcagttttcg gtattattac accaataata ttgcctggtg ttgttctggt aaaacttttt
    12541 ataatacagg aatcttcagg tctgtaggca agatcaatcg ttgcctcaat cgtttcaaat
    12601 ttttctcctg aaatgggttc aaaatgaaat ccagcataaa caacacccct tccttttaac
    12661 tggtcataag tacccagatc aatccaggta ctggtagtgc ctgatgaaaa cgtttctttt
    12721 ggagtaattg tgaaaaacca gcttgggggt ccgtaaagcc catgaatttt caccctgtag
    12781 cttttgtttt caggttgaat taacttgaat ctcacgtatt ccccatattc tttcatttca
    12841 tagatcttaa tgtcgtcaaa atatactgtc ccaatgccat ttgcttcaag acgcaattca
    12901 cagtatgaaa tatttgggtc aggatttttg ggttcaccaa atccgcggct gtgtcttcca
    12961 ccgacaaacg taagtcctgc aattgtccag tcagagtcag gaccaacccg gtttgcatat
    13021 ggctcaccag ttgcaccata aacaactcta aatgttcctg aaatctgtcc gactgttttg
    13081 atttttgcgc tcatgtaata tttcttgccc ttttcaacct ggaacgaacg ggggatgtat
    13141 gttgatgtcc ttttattcag tgtgatagca aaagaataat tgccttcaca ggcgacattt
    13201 tcaaaagaaa atacaacatc aggaagtggt tgtgtttttt cagtcggctt gatgttccat
    13261 cctgctgaca attgttcaaa ccctggattt gtaagtggaa atacttcctg tttgaaaagg
    13321 atattgtttg actgtgcata taacgaaact gacattacaa aaagaataaa aaatactttt
    13381 ctcattttgc ctcccggaag gttttgtcaa tcaaagagta aacaattttt caatcctttc
    13441 aagtggaatt gtaatcaatt caataatttt tttgtttctt gaaaaagcaa tcagtaactg
    13501 acccttgtat tccattgcgt gtggatactg aagtgttgcc tttccgtctg ttggaatatc
    13561 aattcttgca ataccggtga acacccttcc atcagttgaa acagataggt gcatctggcg
    13621 tcttaaaata ttcatatttc tattggcgtt tgaaacaaat accctgaatt ttcggctcgt
    13681 gataagacca aaaaatttac ttgtcgcatt cggaaaattt gtttttacag ggtttgtcca
    13741 tgttttgcca ttg
//
LOCUS       DAORYU010000009        13654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_3546_length_13654_cov_4.102360, whole genome shotgun sequence.
ACCESSION   DAORYU010000009 DAORYU010000000
VERSION     DAORYU010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13654
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3546_length_13654_cov_4.102360"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="PLW07_00700"
     CDS             complement(<1..595)
                     /locus_tag="PLW07_00700"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOL49076.1"
                     /translation="MKKQKFNVLLLLVCLVFMSQGYLFAAEMTENIKKGILKLQMSRL
                     DDAQNYFEREKQSNPNNGLAYYYLGEVYYRKADFSKALEQYQKAIEIEPGNAAYHLGA
                     GVAYLALGQSDKAIEEFQTVVTSAPNTYEATEAQQQLARIKSTNRDSDIVKKWQDAEK
                     NTGVEVAVKKEETIQGPVPAVQTVSVESVVKDMRFGTE"
     gene            complement(576..1622)
                     /gene="prfA"
                     /locus_tag="PLW07_00705"
     CDS             complement(576..1622)
                     /gene="prfA"
                     /locus_tag="PLW07_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOL49077.1"
                     /translation="MVEKIEQLSREYEELIGFVSDPASSNSADYREKIKRIGEISSIV
                     EKGKQYLVLVHKIEQLEELAQDDSLKEIVEEEYTRLTNEIQPVEEELDQYFFSCPDDK
                     KDCIVEIRAGTGGEEASLFAKDLFRMYSKFAESKGLIIEIFDFHASELGGFKEIIFLV
                     RGEYAYGNFKYESGVHRVQRIPDTESSGRIHTSAATVAVFPSVEEDQIKINPEDLKIE
                     TFCSSGHGGQSVNTTYSAVRITHIPTGITVRCQDERSQIQNRARAMKILRARLQDMMQ
                     NQKEQELSRKRKAQIKSGDRSEKIRTYNFPQNRLTDHRIGLTLHNLQEIMEGKLDYLI
                     GELKKEIQNEETKV"
     gene            complement(1624..1839)
                     /gene="rpmE"
                     /locus_tag="PLW07_00710"
     CDS             complement(1624..1839)
                     /gene="rpmE"
                     /locus_tag="PLW07_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOL49078.1"
                     /translation="MKEKIHPEYRESRLVCACGNTVITRSTKPEIKLEICSKCHPFYT
                     GKQKIIDTAGMVEKFRKRYGDKTPGKK"
     gene            complement(1817..2407)
                     /locus_tag="PLW07_00715"
     CDS             complement(1817..2407)
                     /locus_tag="PLW07_00715"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HOL49079.1"
                     /translation="MGTGEMNFQKKIVLGLLILWAILLIVFGLFKAGIFSSKKITVYF
                     FKNQSDGSLTFVEVTRQSKTGLLGSSLEEKIKSAMTELIKGPGSDETTSGYISCVPSE
                     AEILGVKVEDNLIYLDFDENIESGGGISEIRGRLAQIVFTATQFNPDAGVRILIGGKE
                     IKSFSGEGITDVEKPMYRKDFSEFLRGENNEGKNPS"
     gene            complement(2389..3879)
                     /locus_tag="PLW07_00720"
     CDS             complement(2389..3879)
                     /locus_tag="PLW07_00720"
                     /inference="COORDINATES: protein motif:HMM:NF019496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
                     /protein_id="HOL49080.1"
                     /translation="MKNTCVGIVMMNDERQHVWQQNEKENMAVVHQWADFLKKNLKDK
                     TGTCPEIVVGSETIKSIEAARKVGEELVRKGCRQVVMCYNVWDFPYLVWPFIQTIGTD
                     KPVLSLSNNNGAYPGNVGLLATDGALRQAGIRTHRIVGEIGDAVTQQKVLNWVRASQA
                     VTCIKNEVYGCYGGHSMGMETGYFHLVPTVKHFGTTVYQIDQLLLVKKMEQVSLKEIE
                     KGFKWFSEMLGDRIKYDGKMLTPETLKKQIALYLAMKMVNQEYSFDFCGLKGQRELTE
                     HVCLGDVPEMLMNDPYDWNGSKEPFVCATEADSYAALTMQILKYISGGLPVLFMDVRL
                     YHPDLGIWDFCNSGNHASYYASLSDEPKENFSKITFYPALEFYFKAGGASVEFDAAPG
                     TLTFARLGLWDDKPYMVIIKGESLSLDKEIRKKINDETNPTWPHVHARLECNFENFLK
                     VFPCNHILAVSGDCVESLKYFCDICKISAIILDKSAEIGNVWELVK"
     gene            complement(3905..6520)
                     /locus_tag="PLW07_00725"
     CDS             complement(3905..6520)
                     /locus_tag="PLW07_00725"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOL49081.1"
                     /translation="MKNNQETLNFSVRMIHASAGTGKTHRLTREFIALVKKDDFIRNI
                     KRIVAITFSEKAACEMKSRIIHAIFNEILTGIKDEKTRIEYENQLFLLRISTIHSFCK
                     NLLTRFSFLFQIDPNFTVAEPEKIMIFFNQAISKFLENTHFDNPVISRVKPMKMKQFL
                     DRFGYLNKNHPQVFLGKPIDDNETTGPVFECFLIVNNFFQEIKKQQSVMDFNDLEIYT
                     YLLLKEHPESLNVLNDFDEAVDFIFVDEFQDTSLLQWKIIREFSSEWVSGIGAKADTG
                     KSYGLFFVGDRKQSIYHFRGAENTVFDDARAFYGQYVSSEYLTTSYRSFPSVIEFVNR
                     VFEGYPGFPEQEKLRVHSDFANRKEGFVEVKLFEKNTSIFEAKETEFNWIARRILSLI
                     DSQFTVYDKNTKAFRPISFKDIAILMRKRTHLKILEQQLRSYSIPFVNVGGIGFYQEP
                     EIKFLVYLLCALADGSDLLALKNLQQSVFKINLSQIEQWRTLLKSDFAASVLDRIIAE
                     TGMFSVAGTQGCANIEKFLMLVHEMRDIPFFQMVQNFRGILSKEEEPKADVFSEFQDA
                     VRVLTVHGSKGLEFPVVFLSGLEQGIPNKSKITLMHKRNDTPDSDYVFSFRSSGEKGD
                     FYDDYVRRLEEEERRILYVALTRARQSLVITGVKRRSTWFEMLEKFEIDYPATDPMQR
                     EFVVRQQEQQSVSQIPVIRRRPVSPVSFSSQKEQFESQKEKTGTIIHKIICEISNGFV
                     NARHDEMTKRARFLAEKINVNIDYKDLEIHLQNLLKPEILDVISPKPDSYSELPFLVE
                     IDGMYVQGVIDRVVYSNGIWKIYDYKTRHTLAITDDDRKQLKIYREGISHIFGTRNVQ
                     AFLIFTFCGIIKELE"
     gene            complement(6510..9191)
                     /locus_tag="PLW07_00730"
     CDS             complement(6510..9191)
                     /locus_tag="PLW07_00730"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOL49082.1"
                     /translation="MQQKSPMTDTKKYLIVFPFSCKERTGYIIEKFFSDTTDFSHITH
                     LASRWTGIEDFRIKLIMHRRKPVILPSSYSLKSFAAKIVQDHTEYRLISLIEQFLMLL
                     ELCGDMAGKTGINVVSLATRIKSFIKDFKVSHERFDFNDWFSEIQKYPWRYQENKYVV
                     EQAFNVMQKYQNNLENNHLVDEDDLYKIAAEKIKDMNFDTVLLDGMLEFIPSQRLLIK
                     RIAENSNLFISTYQYDESAPYDAKTFVLEPNLNFLKGIADTVINVESKNNDIDAIVYN
                     FPSPDEEVKGIAELILKEMAECGSMNWEDVLVVFPDMLGYRELVQRIFHRMAIPFCMT
                     PGHVLSQDPSIVAIISFLGWLDSLSWEHLMSLFTSPFFSFDRQEAEEFSKQTRKIFKG
                     IGFFPEEKWLRKWKNWKKLEKAAKMMKAKQDSLTNWSENLIKAIQEIGWKEFDIEGRI
                     SFMELMEQLKGKLLLSRTEFVNILKTSLNLTEVEKSKGAGVRVMGVLDSTGIEADIVF
                     MGGATEEALPMACRQEEFFLPDPLKEILCLNTYKLRIARERLDIYRLKMLNKIVFTYP
                     AKIAGRQQNGSIMLHDFQQSAYRKDFYIAMPQQIFNPVPDYRRFEEKFIKNGMIYCTV
                     SQLDALARCPYIFYLTYVEEVESYRAPAIEEVPEFWGVLLHAAAERAAIDFKGKIMDK
                     QSVEAQHKKFCQFVDEFLDNPGAVSHKYFYRIPPVVRSFLKKREQYVFDSFREIIGNH
                     TGHKILDLEKLTTVQIGNIMLTGKFDRMEEREDGTIEIIDFKSGKLPRNVKKYPETGN
                     CFDLDNLELPLYSLMQYKISGKKSRVFAWSLNFEEKENFEIEYSGIATFLERFESDLN
                     KLGEQLLKGQFQFIQKSENCYTCQFSNFCILKGDNEE"
     gene            complement(9219..10541)
                     /locus_tag="PLW07_00735"
     CDS             complement(9219..10541)
                     /locus_tag="PLW07_00735"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOL49083.1"
                     /translation="MLSQQEKSIFFIGQVAGNTYYTVAFNGVLTLFLLKIGVKEGLLG
                     ILSVIPFIVGIASSFLVFWVGKNYTKILRPNACFLTGLAFLLLPLFLITEKIGYSASI
                     IYYAIFVFLFWSGTQISSLAWFPVVQLWVPEEERGRFFGILRFTSTIVGFAFLRLSSY
                     ILGAEPSFREFFIVMLAVSIFAIIWPFSYFHVSMPSPSDEEKTETRMFGIFREIFSDR
                     ERKLYFCFYFLWNFAAYIVAPFVIPFYKTVLHLSSSFCVFLTSMSVLGMGGLAFIWGK
                     VNDFRGSRFVLFFSFLLGLIYCLLMAHIHKFPLQGIEQALIFISLIGGIAGGGQLMGD
                     TTRRFLIAPAKKRVAFFSYLVIFGGQLPALIVPPISGRIIENYKHISISGYGIYQLIF
                     LLIGILHIVFLGMILKMKPIKEKPVGEVFKDVVAENLMKLRDLIASPP"
     gene            complement(10596..11888)
                     /locus_tag="PLW07_00740"
     CDS             complement(10596..11888)
                     /locus_tag="PLW07_00740"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOL49084.1"
                     /translation="MCGLITGTFFFTVCVGPLFTLFLLKLGVREKIIGMLSILPSVVG
                     VFSLWMIRSVAKNPVANFINAAWKLFIIECCFLPVFILAGILPSSIIVVIFSLLLFIF
                     YFPSQQYVYAWFPVIESVIPSHERGHFLGVLRIILTLAGYGLLLICSKILGPDPDYWR
                     FFFALLMIMVISILFPVSLSRTGIPEIKKELSEKIGFLDEFIEILSNKDHAAYFRFLA
                     IWTFITGIAGPFLMPFYKTELGLSTSFCINLASVNTLGYGFSVFGWGRVVDRHGSRYV
                     LFISCCLAVLHLLLLSHVHLFPDQWLKKFLLFISFFGGVVFAGQLMGDTTRRMALCPQ
                     QNKSSYFAYMLVFGAQVPLIVASPLSGFVLQRYRDFQSGFYGIYQSVMLLTGFLYLFL
                     LYQILQMRPVKEKPVMELLKDDITESLLKLRDVIASPP"
     gene            complement(11936..12652)
                     /gene="radC"
                     /locus_tag="PLW07_00745"
     CDS             complement(11936..12652)
                     /gene="radC"
                     /locus_tag="PLW07_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOL49085.1"
                     /translation="MKEKKKVWTVSDLPVDERPRERLLKLGPQSVSTQELLAIIIASG
                     VKGVPVMMAAQSLLSYFGSLSNIADASEEELKSAVKGIGPAKIARLKACFELAKRLMK
                     AENEQPVCRTEAKITNPEVAYSLIKKHIRAYKKEHFFAVSLDSRNRIIGIDEISTGTL
                     EASVVHPRETFESAIKRHAAKIIIAHNHPSEDPAPSEDDIGITTRLKEAGLIMGIELI
                     DHIVICKNSYFSFKEKGLIF"
     gene            complement(12649..12894)
                     /gene="xseB"
                     /locus_tag="PLW07_00750"
     CDS             complement(12649..12894)
                     /gene="xseB"
                     /locus_tag="PLW07_00750"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOL49086.1"
                     /translation="MLKDKEKLKSALQKLERIVEDLGKKDIDVEQGLEKFREGVELIK
                     FCRSQLQKAENEFIQLKQELEQESEGQEPETKEEDNL"
     gene            complement(12888..>13654)
                     /locus_tag="PLW07_00755"
     CDS             complement(12888..>13654)
                     /locus_tag="PLW07_00755"
                     /inference="COORDINATES: protein motif:HMM:NF014640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOL49087.1"
                     /translation="LDVIVLIRGGGSLESLKAFNSESVADAIFDSWLPVITGIGHERD
                     ITIADFVADKSFSTPTAVASFIRTQREQLIQNLDLMVDGFISSMEANFEALQETIQSF
                     QQDILSCFESILERYFFSIRHCAERMHGRLNLVFDSYRMIEKKFLSNLSEWHLHTRQM
                     QGSFFEMRQALLHNFQSLLEKLNNRLKVAEARLTSSNPESILSRGYSIVYDDENKIIK
                     DARKTKQGDRIHIKLHKGKIISTVEQTNNNEGSGTC"
BASE COUNT         4250 a         2948 c         2159 g         4297 t
ORIGIN      
        1 tctctgtgcc aaatctcata tccttgacca cagattcaac agaaacggtt tgaaccgcag
       61 gaacaggacc ctgtatcgtt tcttcctttt tcacagcgac ttcaacccct gtattttttt
      121 ctgcatcctg ccattttttt acgatatctg aatccctgtt agtggactta atcctggcaa
      181 gttgttgctg tgcctcagtt gcctcgtatg tattgggtgc tgaagtaaca actgtttgaa
      241 actcttcaat agctttatct gattgcccga gagcaaggta ggcaacacct gctccaagat
      301 gatatgctgc attacctggc tctatttcta tagctttctg atattgctcc agggcttttg
      361 aaaaatccgc ctttctgtaa taaacctcac ctaaataata ataggcaagt ccattattag
      421 gatttgattg tttttcacgt tcaaaataat tctgggcatc atcaagacgg ctcatctgca
      481 actttagaat tcctttcttt atattttctg tcatttcagc agcaaataaa tatccctgag
      541 acatgaaaac aagacagaca agcagtaaaa gcacattaaa cttttgtttc ttcattttgt
      601 atctcctttt tcaattcacc aatcaagtaa tcaagttttc cttccataat ttcttgtaaa
      661 ttgtgtaaag ttagaccaat tctatggtca gtgagtctat tctgggggaa attgtatgtt
      721 ctgattttct cgcttctatc acctgatttt atctgcgcct ttctttttcg ggaaagttct
      781 tgttctttct gattctgcat catatcctga agccttgccc tcaaaatttt catcgctctt
      841 gcgcgatttt gaatctggct tcgctcatcc tggcacctta cagtgatgcc tgttggaata
      901 tgcgtaattc ttaccgctga ataagtagta ttgacactct ggccaccatg acctgaagaa
      961 cagaatgttt ctattttcaa atcttcagga ttaattttta tctggtcctc ttcaacactg
     1021 ggaaagaccg caacagttgc tgcagacgtg tgaattcttc cagaagattc agtgtcagga
     1081 attctttgaa ctctgtgaac accgctttca tatttaaaat ttccgtatgc gtattcacca
     1141 cgcacaagaa aaatgatttc cttaaatccg cccagttctg acgcatgaaa atcaaaaatt
     1201 tcaataatca atccctttga ttctgcaaat ttactgtaca tcctgaacag gtctttcgca
     1261 aaaagagatg cctcttctcc tcctgtgcca gcgcgaatct caacaatgca atctttttta
     1321 tcatcaggac aggagaagaa gtactggtca agttcttctt caactggttg tatttcgttt
     1381 gtgaggcgtg tgtactcttc ctcaacaatt tccttcagtg aatcgtcctg cgcaagttct
     1441 tcaagttgtt caatcttgtg gacaagaacc agatattgtt tacctttttc aacaatactg
     1501 gagatttcac cgattctctt gattttttct ctgtaatccg cagaattact gctggcagga
     1561 tcagaaacaa accctatcag ttcttcatac tccctgctca attgttcgat tttttcaacc
     1621 agatcatttc tttcctggtg ttttatcacc atatctcttc ctgaatttct ccaccattcc
     1681 ggctgtatca ataattttct gctttccggt ataaaacgga tggcattttg aacaaatttc
     1741 aagctttatc tctggctttg tggaacgggt tattacagta ttgccacagg cacaaacaag
     1801 tcttgactcc ctgtactcag gatggatttt ttccttcatt gttttctcct ctcaaaaatt
     1861 ctgaaaaatc ttttctatac attggttttt caacatccgt aattccttct ccgctgaaac
     1921 tttttatctc ttttcctcca atcagaatcc tgactcctgc gtccggattg aattgtgttg
     1981 cagtgaaaac gatttgagca agacgccccc tgatttctga aatgccacca cctgattcaa
     2041 tattttcatc aaaatccagg taaatcaaat tatcttcaac cttcacacca agaatttctg
     2101 cctctgaagg tacgcaggaa atatatcctg atgttgtctc atcagaacct ggccctttga
     2161 tgagttcagt catagcgctt tttatttttt cttccaggga actgcccaat agaccagttt
     2221 tagattgtct tgtcacttca acaaaagtca aggaaccatc tgactggttt ttgaaaaaat
     2281 atacggtgat tttttttgaa gaaaatatgc cggctttgaa aagcccaaat acaatgagca
     2341 atatcgccca cagaattaaa agacccagca caattttctt ttgaaaattc atttcaccag
     2401 ttcccataca tttccaattt ctgcgctttt atccagaatg atcgctgata ttttgcatat
     2461 atcacagaag tattttagag attcaacaca atctcctgac actgcaagaa tgtgattgca
     2521 gggaaaaacc ttaaggaaat tttcaaagtt gcattcaagt cgtgcatgaa catgcggcca
     2581 ggttggattt gtctcgtcat taattttttt cctgatctct ttatcaagag ataagctttc
     2641 accttttata ataaccatat atggtttgtc gtcccacaat cccaaccttg caaatgttaa
     2701 tgtcccgggc gcagcgtcaa attctacaga agcgccacct gccttaaagt aaaactcaag
     2761 tgctggataa aatgtaattt ttgaaaaatt ctccttcggt tcatcactca aagacgcata
     2821 ataacttgca tggtttccgg aattacagaa atcccagata ccaaggtctg gatgatacaa
     2881 tcttacgtcc ataaaaagca caggcaatcc accgcttata tatttaagga tctgcattgt
     2941 caatgccgca tatgaatcag cttcagtagc acaaacaaat ggttcttttg atccattcca
     3001 gtcgtacggg tcattcatca acatttctgg aacatcacca agacaaacgt gttcagtcaa
     3061 ctccctctga cctttaagtc cgcagaaatc aaaactgtac tcctgattaa ccattttcat
     3121 tgccagatac aatgctatct gtttttttaa ggtctctggt gtcaacattt tcccatcata
     3181 tttaattctg tcgccaagca tttcagaaaa ccatttgaaa cccttctcaa tttcttttaa
     3241 agaaacctgt tccatctttt ttaccagaag caactggtca atttgataca ctgttgtccc
     3301 gaagtgtttg actgtcggca caagatggaa ataaccggtt tccataccca ttgaatgacc
     3361 accataacaa ccgtacactt cattttttat acatgttact gcctgcgatg ccctgaccca
     3421 gttcaacacc ttttgctgtg tcacagcatc accaatttct ccaacaattc tgtgggttct
     3481 gatacctgcc tgcctcaatg ccccatctgt agcaagaagc ccaacatttc caggatacgc
     3541 gccattgtta ttggaaaggg ataatacagg tttatcggtt ccgattgttt gaataaatgg
     3601 ccagacaaga tacggaaaat cccagacgtt ataacacatc actacctgcc tgcatccctt
     3661 tctaacaagc tcttcaccaa cttttcttgc tgcttcaata cttttgattg tttctgaacc
     3721 caccacaatc tcaggacatg ttcctgtttt atcttttaaa ttctttttca aaaagtccgc
     3781 ccactgatgc acaacagcca tattctcttt ttcattctgc tgccatacat gctgtctttc
     3841 atcattcatc atcacaatac ccacacaggt atttttcatt tctaccccct ttaagatatt
     3901 atgattattc tagttctttt attataccgc aaaatgtaaa aataaggaat gcctgaacat
     3961 ttctggtacc aaaaatatgg ctgatacctt ctctgtatat ttttagttgt ttcctatcat
     4021 catctgtaat ggcgagtgta tgccttgttt tatagtcata aatcttccag ataccattgc
     4081 tataaacaac acggtcaata acaccctgaa catacattcc atcaatctca acaagaaatg
     4141 gcagttcact ataagaatca ggttttgggg aaataacatc aagaatttct ggcttcaata
     4201 aattttgaag gtgtatttca aggtctttgt aatctatgtt tacattgatt ttttctgcta
     4261 aaaatcttgc ccgttttgtc atttcatcat gtctggcatt gacgaaaccg tttgatattt
     4321 cacatattat tttatggatg atagtacctg ttttttcttt ctgagattca aactgttcct
     4381 tctgtgatga gaaagaaacc ggtgacacag gtctcctgcg aataacagga atctgagaaa
     4441 cagattgttg ttcttgctgg cggacaacaa attctctctg catcgggtca gtagcaggat
     4501 aatctatttc gaatttctca agcatttcaa accaggtact ccttcttttc actcctgtta
     4561 ttacaagaga ttgccttgcc cttgtcaggg caacatacag aattcttctt tcttcctcct
     4621 caagtcttct cacgtaatca tcataaaaat ctcctttttc tcctgaactg cgaaacgaaa
     4681 aaacatagtc agaatccggg gtatcatttc ttttatgcat cagggtaatt ttgcttttat
     4741 tgggaatacc ctgttccagt ccagataaaa acacaaccgg gaattcaagt ccctttgaac
     4801 catgcactgt aaggactctc acagcgtcct gaaattctga aaaaacatct gcctttggct
     4861 cttcttcctt acttaatata cctctaaaat tctgaaccat ctgaaaaaac gggatatccc
     4921 tcatttcatg aaccagcatc aaaaattttt caatgtttgc acaaccctga gtgccagcaa
     4981 cagaaaacat gccagtttct gcaataatcc tgtcaaggac agaagccgca aaatcacttt
     5041 tcaaaagggt tcgccactgt tcaatctgag atagattaat tttgaatact gactgctgaa
     5101 gatttttcaa agcaagcaaa tccgaaccat cagcaagagc acaaagaaga tagacaagaa
     5161 attttatttc tggctcctga taaaatccta ttccaccgac atttacaaac ggaatagaat
     5221 aactccgcaa ttgttgttca agaatcttca aatgggttct cttgcgcatc aaaatggcta
     5281 tatccttaaa acttattgga cgaaatgctt tcgtgttttt gtcataaaca gtaaattgcg
     5341 aatctatcag ggacaaaatc cttcttgcga tccagttaaa ctcggtttcc tttgcttcaa
     5401 atatagaagt atttttttca aaaagtttca cctcaacaaa accctccttc ctgtttgcaa
     5461 agtcgctatg cacacgaagt ttttcctgtt caggaaatcc cggataacct tcaaacactc
     5521 tgttgacaaa ttcaatgaca gacgggaaac tcctataact ggtcgtcaga tattcagagg
     5581 aaacgtactg gccataaaac gctctggcat catcaaaaac agtattttca gcaccgcgaa
     5641 aatggtatat ggattgtttt ctgtccccga caaagaataa cccataactt tttccagtat
     5701 ctgcttttgc accaattcca gaaacccatt cagaagaaaa ttctctgatg attttccact
     5761 gaagcaaact tgtatcctga aattcatcaa caaaaatgaa atccactgcc tcatcaaaat
     5821 cattcagaac attcaaggac tctggatgct cttttaacag aaggtacgta tagatttcaa
     5881 ggtcattaaa atccatgact gattgctgtt tttttatctc ctggaaaaaa ttgttcacaa
     5941 tcagaaaaca ctcaaatact ggtcctgttg tttcgttatc atcaatcggt tttccaagaa
     6001 acacctgcgg atgattctta tttaaatatc caaacctatc aagaaactgt ttcattttca
     6061 ttggttttac acgcgatatt accgggttat caaaatgtgt gttttcaaga aattttgaaa
     6121 ttgcctgatt gaaaaaaatc attatttttt caggttctgc aactgtaaaa tttgggtcaa
     6181 tttgaaacaa aaaagaaaag cgtgtaagaa ggtttttaca gaatgaatgg atggttgata
     6241 ttcttaaaag aaacagttga ttctcgtatt caatccgtgt tttctcatct tttattccag
     6301 tcaaaatttc attaaaaatg gcatgaataa ttcttgattt catttcacat gcagcctttt
     6361 cagagaaagt aattgcgaca attcttttaa tattacgaat aaaatcgtcc ttttttacca
     6421 gagcaataaa ttctcgtgta agcctgtggg tttttccggt acctgcagac gcgtgaatca
     6481 ttctaacaga aaaatttaat gtttcctgat tattcttcat tatcaccctt taagatacaa
     6541 aaatttgaaa attgacaggt gtaacaattc tctgatttct gtataaattg aaactgtcct
     6601 ttgagcaact gttctccaag tttatttaag tcagattcaa atctttcaag aaatgttgca
     6661 atacctgagt attcaatttc aaaattttct ttttcttcaa aatttaaaga ccaggcaaat
     6721 acacgacttt tttttcccga aattttgtac tgcatcaggg agtaaagcgg taattccaga
     6781 ttatcaagat caaaacaatt ccctgtttct gggtattttt taacatttct gggaagtttg
     6841 ccactcttaa aatcaataat ttcaatggtc ccatcttccc tttcttccat cctgtcgaac
     6901 ttaccggtca gcatgatatt gcctatctga accgtggtca gtttttcaag gtccaaaatt
     6961 ttgtgacctg tgtgattgcc gatgatttct ctaaaagaat caaacacata ctgctctctt
     7021 ttttttagaa aacttctcac cacaggtgga atcctgtaaa agtatttatg agaaactgcc
     7081 cccgggttat cgagaaactc atcaacaaac tgacagaact ttttatgttg cgcttcgaca
     7141 gattgtttat ccattatttt tcctttaaaa tcaatggctg ctctttctgc agcagcgtgc
     7201 aacagaacac cccagaattc tggaacttct tcaatggcag gtgctctgta tgattccact
     7261 tcttccacat aggtaaggta aaaaatatac ggacatctgg caagagcatc aagctgactc
     7321 actgtacaat aaatcattcc attttttatg aatttttctt caaatctacg atagtcagga
     7381 actggattga aaatctgttg tggcatggca atgtaaaaat cttttctgta cgcggattgc
     7441 tggaaatcgt gaagcataat ggaaccattc tgttgccttc cagcaatttt tgcaggatat
     7501 gtaaatacaa ttttatttag cattttcaac ctgtaaatat ccaatctttc ccttgcaatg
     7561 cgcagtttgt aggtattgag acagaggatt tcctttaatg ggtctggcag aaaaaattct
     7621 tcctgacggc atgccattgg aagtgcttcc tcagttgcac cacccataaa aacaatatcc
     7681 gcctcaatac ctgtggaatc aagaacaccc attaccctaa ctcctgcccc cttgcttttt
     7741 tcaacttctg tcagattcaa cgatgttttc aaaatattca caaattctgt tctactcaac
     7801 aataatttgc ctttcaactg ttccatcaac tccatgaatg aaattctgcc ttcaatatca
     7861 aactctttcc agccaatttc ctgtattgct tttatcaaat tttcactcca attggtaaga
     7921 gagtcctgtt ttgctttcat catttttgct gctttttcaa gttttttcca gtttttccat
     7981 tttcttaacc atttttcttc agggaaaaat ccaatacctt taaagatttt ccttgtctgt
     8041 tttgaaaatt cttcagcctc ctgtctatca aaagaaaaaa atggacttgt gaacaaagac
     8101 attaaatgtt cccaggataa tgaatcaagc cacccaagaa atgaaataat ggcaacgatt
     8161 gatgggtcct gacttagaac atgtccgggt gtcatgcaaa agggaatagc catacgatga
     8221 aaaattctct gaacaagttc tcgatatccg agcatgtcag gaaaaacaac aagcacatct
     8281 tcccagttca tacttccaca ctcagccatt tctttcaaga tcaattcggc aataccttta
     8341 acttcctcat caggagaagg gaaattatat acaattgcat ctatatcatt gttctttgac
     8401 tccacattga tcacagtatc agcaatacct ttaagaaagt tcagatttgg ctcaagaaca
     8461 aatgtttttg cgtcataggg cgcactttca tcatattgat atgtagatat aaacaggttt
     8521 gaattttcag caattctttt tatcaataat ctctgagacg gaataaattc aagcattccg
     8581 tcaagcagca ctgtatcaaa attcatatcc tttatctttt ctgctgcaat tttgtaaagg
     8641 tcatcctcgt caacaagatg attgttttca agattatttt gatatttttg catcacatta
     8701 aatgcctgtt caacaacata tttattttcc tgatatctcc atggatattt ttgaatctca
     8761 gaaaaccagt cattaaagtc aaatctttcg tgcgacacct tgaaatcctt gataaatgat
     8821 tttattctgg tcgcaagcga cacaacattt attcctgttt ttcctgccat atcgccacac
     8881 aattccagta gcattaaaaa ctgttcaata agggatatta acctgtattc tgtatgatcc
     8941 tgaacaattt tcgcagcaaa ggacttcaga gaataacttg acgggaggat caccggtttt
     9001 cttctatgca tgataagttt tattctaaaa tcctcaattc ctgtccagcg agaagccagg
     9061 tgagtgatgt gggaaaagtc tgttgtatca ctaaaaaatt tctcaataat gtatcctgtt
     9121 cgctctttgc aggagaaggg gaaaacaatc agatattttt ttgtgtctgt catgggactt
     9181 ttttgttgca ttattttatc acacatcata aaactctgtc atggcggact ggcaatcaaa
     9241 tccctcagtt tcatcaaatt ttcagccaca acatccttga acacttctcc gacaggtttt
     9301 tcctttatcg gtttcatttt caaaatcata ccaaggaaaa caatgtgtaa aataccaatc
     9361 agaagaaaaa tcagttgata aataccatag ccagagatag aaatatgttt ataattttct
     9421 atgattctcc cgctgatagg aggcacaatt aaagcaggta gttgtccacc aaaaattacc
     9481 agatacgaaa aaaacgcaac gcgctttttc gcaggagcaa tcaaaaatct tcttgttgta
     9541 tctcccatta actgaccgcc accagcaata cccccaatca atgaaataaa aattaatgcc
     9601 tgttcaatcc cctgtaaagg aaacttatga atatgcgcca tcaggagaca gtaaattaac
     9661 ccgagaagga atgaaaaaaa taatacaaat ctacttcccc gaaaatcatt cacctttccc
     9721 cagataaatg ccagaccacc cataccaaga acagacatcg aggtcaaaaa cacacaaaag
     9781 gaagaagaaa gatgtagcac ggtcttataa aatggaataa caaaaggggc aacaatataa
     9841 gcagcaaaat tccataggaa ataaaaacaa aaataaagtt ttctttctct gtctgaaaaa
     9901 atttcgcgaa aaataccaaa cattctggtt tcagtttttt cttcatcaga tggagaaggc
     9961 attgaaacat gaaaataact gaatggccag ataattgcaa aaatacttac agcaagcatt
    10021 acaataaaaa attccctgaa tgaaggttca gccccaagaa tataagaaga taatctcaaa
    10081 aaagcaaaac caactatggt ggacgtaaat ctcaaaatcc cgaaaaacct tcccctttct
    10141 tcttctggaa cccacagttg taccacagga aaccaggcaa gagatgaaat ctgagtccca
    10201 gaccagaaaa ggaaaacaaa gatagcataa tatatgatgc ttgctgaata accaattttt
    10261 tcagtaatga gaaaaagagg aagaagcaaa aaagccaggc ctgtgagaaa acatgcattg
    10321 ggacgtaaaa tctttgtgta atttttcccc acccagaaaa cgagaaaaga ggaagcgata
    10381 cccacaataa atggtattac tgaaagaata ccaagaagtc cttccttgac acctatttta
    10441 agaagaaaaa gagtaagaac accattgaat gccactgtgt aatatgtatt cccggcaacc
    10501 tgtcctataa aaaaaatact tttttcctgt tgtgaaagca tatctcatcc ctgattaaac
    10561 ttgattatac acctgaaaaa gaaaatgata aatgtttatg gagggctcgc aattacatcc
    10621 ctgagtttta acaaactttc tgttatatcg tctttcaaaa gctccataac aggtttttct
    10681 ttcactggcc gcatttgaag aatctgataa agcaaaaaaa gataaagaaa cccggtcaaa
    10741 agcatcactg actgataaat cccataaaac ccggattgaa aatccctgta tcgttgaaga
    10801 acaaaacccg aaagaggaga agcaacaata agaggcacct gcgcaccgaa cacaagcata
    10861 tatgcaaaat atgatgattt attctgttgt gggcacagag ccattcttct tgttgtatct
    10921 cccatcaact gacccgcaaa aacaacgcca ccgaaaaatg atataaaaag cagaaatttt
    10981 ttcaaccact ggtctggaaa caaatgaaca tgagaaagta acaacagatg caacactgca
    11041 agacaacatg aaataaacaa cacatatctg ctcccatgcc tgtcaacaac cctaccccat
    11101 ccaaatacag aaaaaccata cccaagtgtg ttgactgatg ccagatttat acaaaatgat
    11161 gttgaaaggc ctaattctgt tttataaaat ggcatcaaaa atggtcctgc gattcctgtg
    11221 atgaatgtcc atatagcaag aaatctgaaa taagcagcat ggtctttatt acttaaaatc
    11281 tctataaatt catccagaaa tcctattttt tcagataatt ctttttttat ttcaggaata
    11341 ccagtgcggg aaagtgaaac aggaaacaaa atagaaatca ccatgatcat aagcaatgcg
    11401 aaaaaaaatc tccagtagtc cgggtctggt ccgagtattt ttgaacaaat aagaagcaac
    11461 ccatatccag caagtgtgag aataattctc aacactccaa ggaaatgtcc tctttcgtga
    11521 gaaggaataa ctgattcgat aactggaaac caggcataca catactgctg gctcggaaaa
    11581 taaaaaataa atagcaacaa actgaatatc acaacaatga ttgatgatgg caaaatcccg
    11641 gcaagaataa aaacaggaag aaagcagcat tcaataataa acaatttcca cgcagcatta
    11701 ataaaatttg ccacaggatt ttttgcgaca ctgcgtatca tccacagaga aaaaacacca
    11761 actactgaag gcaaaattga tagcattcca ataatttttt ctcttacacc caatttcaac
    11821 aaaaataggg taaacagtgg acccacgcat acagtgaaaa agaacgtacc tgtaatcagt
    11881 ccacacatta aaataaatct ctgacgggat gatagcatct gtgaaatatg gaaaattaaa
    11941 aaatcaaacc tttttcctta aaactgaaat atgaattttt gcaaatcaca atgtggtcaa
    12001 tcaattctat tcccataatc agccctgctt ccttcaacct cgttgtaata ccgatgtcgt
    12061 cttcagaagg agcagggtct tcagacggat gattgtgagc aataataatt tttgccgcat
    12121 gacgctttat tgctgattca aatgtctcac gaggatgaac aacacttgct tcaagggtac
    12181 cagtggatat ttcatctatg ccaatgattc tatttcttga atcaagagaa accgcaaaaa
    12241 aatgctcttt tttgtaggcg cgaatgtgtt tttttattaa cgaatacgca acttcaggat
    12301 ttgtaatttt tgcctctgtc cggcatacag gttgttcatt ttctgctttc atcaacctct
    12361 ttgccagctc aaaacatgct ttcaatcttg caattttggc tggacctatt cctttcactg
    12421 cacttttcaa ctcttcttct gaagcatctg caatgtttga aagcgaacca aaatatgaaa
    12481 gcagtgattg agcagccatc atcacaggta cccctttaac tccactggca ataataatag
    12541 ccagaagttc ctgggttgaa accgactgag gtcctaattt caacaacctt tcccttggcc
    12601 tttcatcaac cggtaaatca gatactgtcc agaccttttt cttttctttc acagattgtc
    12661 ttcctctttg gtttcgggtt cttgcccctc agattcctgt tcaagttcct gtttcaattg
    12721 tataaattcg ttttcagcct tttgtagctg gcttctgcaa aacttaatca attccactcc
    12781 ttcccgaaac ttttcaagtc cctgttcaac atcgatatct tttttcccca ggtcttcaac
    12841 tatcctttca agtttctgaa gtgcactttt taacttttct ttatccttta acatgtccca
    12901 ctcccttcat tgttattagt ttgttcaact gttgaaataa tttttccttt gtgaagtttg
    12961 atatgaattc tatcgccctg ctttgtttta cgtgcatcct taataatttt gttttcatca
    13021 tcatacacaa tgctatagcc acggcttaaa attgattcag gatttgatga ggttaacctt
    13081 gcttctgcga cttttaaccg attattcaac ttttcaagca aagattgaaa attgtgaagt
    13141 agtgcctgcc gcatttcgaa aaatgaaccc tgcatctgtc ttgtatgaag atgccattca
    13201 gaaaggttgc tgagaaattt cttctcaatc attctgtacg agtcaaaaac caggttcaac
    13261 cgaccatgca ttcgttcagc gcagtgcctg attgaaaaaa agtaccgttc cagaatgctt
    13321 tcaaaacagg ataaaatatc ctgttgaaaa gattggatag tttcctgaag tgcctcaaaa
    13381 tttgcctcca ttgaagaaat aaatccatct accatcaaat caagattttg aatcaactgt
    13441 tccctctggg ttcttataaa agaagcgaca gcagtcgggg ttgaaaaact tttgtctgcg
    13501 acaaaatctg caattgttat atctctttca tgtccaatac cagtaatcac aggcaaccat
    13561 gaatcaaaaa ttgcatcagc cactgattcg gaattaaatg ccttcaaact ttcaagagac
    13621 ccaccacccc tgattaaaac aatcacatca agat
//
LOCUS       DAORYU010000010        13190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_3684_length_13190_cov_4.925847, whole genome shotgun sequence.
ACCESSION   DAORYU010000010 DAORYU010000000
VERSION     DAORYU010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13190
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3684_length_13190_cov_4.925847"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="PLW07_00760"
     CDS             <1..917
                     /locus_tag="PLW07_00760"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOL49088.1"
                     /translation="LNAAANLSDVYSEEDTTTAQVFLRAMRFIDENRVFKPLFLFIDC
                     FIPHEPWKAPDSYLEMYYNNPDYNGRTFLHTKYGPVDTQMTEEEFKHTVAHYCGLVSM
                     ADTWFGYFIQKVKRLGLWENSVIVVLSDHGTNFAENPERVIGKPHYALYPAVMKVPLM
                     IHVPGMKTSRRCKHLLYNIDATATIYTLAGIEKKILLDGKDLTSLMRKNQEEGRQYLT
                     CRYGDSVWYRDTNHWVIMNIKGQARSVFDIKKDPECMYNIVSDSGKIIEKAWKFILKD
                     AEGQLPDYTDLIEETDALGERVWKKRNS"
     gene            complement(927..2324)
                     /locus_tag="PLW07_00765"
     CDS             complement(927..2324)
                     /locus_tag="PLW07_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49089.1"
                     /translation="MRTNSACHQLFVDDAIIEYSNNLQRKYWSPERIGVILKPDKPWE
                     QSCLILYGSVMKDPVMKIFKMWYSSFCGKRNLGRDILYAESSDGFHWSKPELGLIDFK
                     GSTNNNIVIPAEHPHHPDAASVIYDTVDKKKSRRYKMISCRTDHHGRNHIWANFSADG
                     IKWQGWNGPILFGYGDRNTLMFDSSLEKPYVIFTRPPDMMKKFGKRIIVRLDSKDFVR
                     WTPSEMILKPDLDDPVDLQFYCLVAFRYHSMYLGFLQRYHSVEDRLDIELISSRDSIV
                     WQRNRLRCAFMESNFEKSIDSRWIGLASNPPLKINNHLLFFYEARNLCHGLPDSVSLP
                     HGIIGIATMPEDGFVSLSSGSASGELITKSFVCPGGALYLCVNAANHQHSSTHDITHG
                     KIQVEILTTNNSLIPGYSLKECKTIKGDFRTGVVVEWKKKKNLDVLKGQKIRLRFNLV
                     NSDLYSFWFENCSRA"
     gene            complement(2321..2971)
                     /locus_tag="PLW07_00770"
     CDS             complement(2321..2971)
                     /locus_tag="PLW07_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49090.1"
                     /translation="MKNMVFALGILICFCSIAIAEENMMKTLAIEFEDTLTDTCGHNP
                     VIAKNIKFVEGKTGKGIVLDKDAVLAFETKNLLTSKRGIIDFWFNPYWDGVEKKTRFL
                     MVDDENHFQIMKTEYGTICFRIFTDDWQNGLLLSIPVNNWKQGEWHHIIVDWDIEGET
                     VLKIDQQVSKGWIKSGTSTITLGKTIFFGPSRYANTDSVANGIIDQLMMFGKKNEK"
     gene            complement(2986..6699)
                     /locus_tag="PLW07_00775"
     CDS             complement(2986..6699)
                     /locus_tag="PLW07_00775"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HOL49091.1"
                     /translation="MIRTFSPELQDNGMLVNGRTLKCFVFFTFVIILNNLPVAYSQKT
                     TLDSSIYCLDFGLQTEEPEPGFVRVSPARLWSIENGYGWIKKGILATSRRVPDEFRKD
                     KLEYSFHEGTGENTFRVSLTPGKYRCWLVAGDMTYIPPAFSVIVNGKTVISQKETTNY
                     IPRVQTWLFDIDARENNLDFEFKGNPHFVVCGIILAPQNLWYKAAPIIEKKEKELILA
                     PKEQLVSWSEKTHSSQHARPKNSKEIQNGFIVSFWPSYLPVYPNSVPAQERQNFPIWS
                     FGTPGELEPVCFVLCPLRNDVSIVSASITDLKKDSRTKIPNSFLTLRVATYRAHRTLW
                     ATRKEYEWVPELLEKFKAPLRIDETKFFWLTVNVPEKSEPGLYRGSVIFETSEFRTVK
                     VPIMFVVLPFNLPEESRKIFGFHYLDPLYILSHPDINRLYRERQTLSRLEELLQRDLV
                     DMKKHGISSICNPGFRLRISKKGDQIEYTLPVELIEKMIQSGVIKHSFFGITPMIEDI
                     CAITGDPVPHPRGSQEIPPPFSNEFNRIYVNGIKFIQQTAQERKWPEILFYVVDEPCD
                     PERQEIARRLCNLVHQVPGARTLVTGRLGEGYLNEDLVKLIDVIKFSGPESVPDQPEL
                     QKGNWFYPNDVAAASGSYSSVRFLVGFGFWFSQLVGVAPWRYNNIVGSPDTDLDGNMS
                     DFFLTYPVADENIPTLRWENWREGFDDCRYMTLLENKIQKGKTSNVPVAEKAARQAEQ
                     EIADIKSIVPTLNQFASGMISEAGYIKKSGEPLSCEEWMDMYCQKIRWAVANHIIAID
                     RALEGKTSLSAKKMKPVIEVLKQTDARIQKTYWKEEITGAEKVTQPVVIDGDFTEECW
                     KHAKVLHLVNRTDGSIPRKKTEVMVAADNENLYFAFKNFEPAMNNLVAHVTNRDGEVW
                     NDDCVEVFIGNDTGCQNFYQFVVNANGATADAFYLSGEFKPKWNSENVEAAVKKFPFY
                     WQVELKFPRKDVGLSKASIAGINMCREEKQTGELTCWSTSGRGFAYTADFGRLNISDS
                     DCYFSKVLLPESNIGQNQGSIEISNRCDKDKNVTIVVTLYNQHGIPVQQTTRNVSVSR
                     FSMTCVPYEFNLETDESFELQLKLLDQIQNTLYDSKTLRIDSNQIFQIYPDTYYLIKG
                     IDVLQVFCHISIDKKLSGSSPITLTLADNEEKKIFYRKQIPVDEYSLRDFIIRIDPKN
                     LPSGKILATVEVKPGKISRMKKDFAFCVLNGFQ"
     gene            6810..7997
                     /locus_tag="PLW07_00780"
     CDS             6810..7997
                     /locus_tag="PLW07_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017323733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HOL49092.1"
                     /translation="MNKEIFQKYEQQYMASLLHDSIPFCMKHAPDGKYGGYFQCLDFD
                     GSVVSTDKFMWSQNRVVWIFSMLYNNLDKKPEWLEMAQLGIDFLKKYGRDENGDWFFA
                     LDRKGKPIVRAYNIFSDFFAVMAFAEYARASGDKECAEIAAKTYRRIQLRKDNPKGQY
                     NKIITENRNLKNLAFPMINLNVATILANANHDGAYNKLITECVNEIMNQFLDRKHRVV
                     RENIAVDDTSVDEIYEGRHVNPGHGIEAMWFVMQAARKNNDQATIREACEAVKWLLEF
                     GWDEQYGGIFYFLDLYGKPHIELCWDMKLEWVHVEALVATLMGYYLTGDSELFDWFEK
                     IHDYTWQHFPDREYGEWYKYLNRRGEVNNRCKSNKWQNFFHLPRALYLIAKICRALQD
                     TDL"
     gene            complement(8025..9062)
                     /locus_tag="PLW07_00785"
     CDS             complement(8025..9062)
                     /locus_tag="PLW07_00785"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOL49093.1"
                     /translation="MNASESIFRKRVFTALSFREPDICPYYIWIDPAMLERLTNHYRN
                     PELKKNYIYDHTVMIEITAEQCMLPDGTSRDEFGTIWQKGSILHIVKPALTEPSLSGY
                     RFPDLTTFSHYAHLDKWLTENNDRFKIVQLGLLFFERTWAMRGFQEIMMDFCENPEFV
                     EELLDHLAEISIQIIDKLIEHYGNSIDAIGFSDDYGGEHAMLLSPAMWRRFIKPRIKK
                     MYARIKKAGKKVYLHSCGHISPIIPDLIEAGVDILQPIQPETMDIFALKKHFGRDLCF
                     AGGISTQKTLPFGTPQQIRDEVNRCLDIMGKDGGYIIAPAKPILPDVPVVNAVSLIDH
                     IVSQKPKSNLG"
     gene            9271..10557
                     /locus_tag="PLW07_00790"
     CDS             9271..10557
                     /locus_tag="PLW07_00790"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOL49094.1"
                     /translation="MKQKLSFNTKEYQQLISIFNSLDEMIYVTDPETYEILYINPVLK
                     KTFGDVIGKKCYRVFQNKNKPCSFCTNKFIFGPEAKPVYIWEFQNKVNKRWYRCIDRP
                     IEWIDGRKVRFELAIDITDKKLMEEFLLESQQRYRDLWDNAPIAYHIVDKKGIIRDVN
                     KTELKLLGYSKKEMLGKPIFDFIIPSQREEARERFMLKMKGKKVDKKYDRIYMKKNGT
                     SLYVSIHDVPEKNEKGEIIGMRTAMIDITELKKMEENLKEISLKDELTEVYNRRGFFT
                     LAEQEIRRTQRGKNPFYILFIDFDNLKSINDKKGHLCGDEVLKKLGEILKDVFRQSDI
                     IARIGGDEFVVLASDVKGSKEVKVLIDRMRKTIQTFNKGNNKYQISISIGTARYDGTK
                     PVTIDGLLVEADRKMYQEKIAKYKLAKYQSRTIFDL"
     gene            10700..10771
                     /locus_tag="PLW07_00795"
     tRNA            10700..10771
                     /locus_tag="PLW07_00795"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10732..10734,aa:Thr,seq:tgt)
     gene            10904..12400
                     /locus_tag="PLW07_00800"
     CDS             10904..12400
                     /locus_tag="PLW07_00800"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046353 - aminoglycoside
                     3-N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HOL49095.1"
                     /translation="METIVTKKDIVSGLKKLGLKKGDIVLVHSSLSSLGKVEGGPETV
                     IQALIEVVGRTGTIMMPYPLGNATIAKVFSSMKGVIKSIHPVHSVSAMGARARYLTKD
                     HDKMPTACGKGTPFGKLVDLGGYILLMGVDQDRNTTLHTAEDYADLPYLSEKEFKYVD
                     ENGVEKTIVLKRFPGPHRNFIGMDRIFLEKKIMNIERIGNAMVRLIDAKKMIRVCLDE
                     LKKNPAAFLCENPNCADCVMQRGKIKEARLKKECFTLSATTSHIASEPEEMLKILQGQ
                     GVKTIELDVIWNKPVTTLAPSELQYLKDLFAKHNFSVAGISCKEDCDIEKCLDIAEFF
                     GAKYTVLPNLIFSQDIQKQKRQFIESLFPVIEIAEKKNIVLLIENRPATVCARSSDCL
                     EILGKIKSPHLKLAFNPANFAIAGEKPFLGISPHVRRMAFVLYVNDALFTGKPQFPGY
                     GNAEIKELMSILRCWCFSGYFCLKPGIGTGKEFFNSAADAFWHLLETM"
     gene            12405..13016
                     /locus_tag="PLW07_00805"
     CDS             12405..13016
                     /locus_tag="PLW07_00805"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOL49096.1"
                     /translation="MKIIDLTREISKDFRTDIVNIRLSGTEYHAFVHQFHLSSMAGTY
                     IDFPGHIKEFDNGFDSSNYPVEKLFMVDATVIRLNRSGKQREIHAEELDAANVVLDTP
                     AVIVDAGWNLLKEKNQDEIYFFGKDAISWFISKNIYLFVSDVYENHKDPRGIFVEFFS
                     KGILTVCNPVNLEKIAAPKVKLCVFPLRIPGVTQVPCRVIAVM"
BASE COUNT         4025 a         2485 c         2666 g         4014 t
ORIGIN      
        1 cccttaatgc cgcggcaaat ctttcagatg tttattctga agaggatacc accactgctc
       61 aggtttttct cagggcaatg aggtttattg acgaaaacag agttttcaag ccattgttcc
      121 tttttataga ttgtttcatt cctcatgagc catggaaggc acctgattca tatcttgaga
      181 tgtactataa caaccctgat tacaatggca ggacttttct ccatacaaaa tatggacctg
      241 tggacacaca gatgacagaa gaagaattca aacacactgt tgcacattat tgcgggcttg
      301 tttcaatggc tgacacatgg tttggctact tcattcagaa agtgaaaaga ttaggtctat
      361 gggaaaattc tgttattgtg gtgttaagtg atcatggcac gaattttgct gaaaatcctg
      421 aaagggttat tggtaagccc cattacgctc tttatcctgc tgtaatgaag gtgcctctca
      481 tgatacatgt acctggtatg aagacgtcca gaagatgtaa acatttactt tataatattg
      541 atgccactgc cacaatctac acactggcag gaattgaaaa aaagattttg ctggatggca
      601 aagatttgac atctctgatg aggaaaaatc aagaggaagg caggcagtat ctcacttgtc
      661 gctacggtga ttcagtctgg tacagggata caaaccactg ggtgattatg aatataaagg
      721 gacaggcaag aagtgtgttt gatatcaaaa aagaccctga atgcatgtac aatattgttt
      781 cagatagtgg aaaaatcatt gaaaaagcat ggaaatttat tcttaaagat gcagaaggac
      841 aattgcctga ttacacagat ctgattgaag aaactgatgc gcttggagaa agggtctgga
      901 aaaaaagaaa cagttgaaga accaggtcat gctctgctac agttttcaaa ccagaaagag
      961 tagaggtcgc tgttgacaag attaaacctc aggcgtattt tttgtccttt aagtacatcg
     1021 agattttttt tcttcttcca ctccacaacc acgcctgttc tgaaatctcc ttttatggtt
     1081 ttacattctt tgagagagta tccgggaatc agagagttat ttgttgtaag aatttctacc
     1141 tgtatttttc cgtgagtaat gtcgtgtgta cttgaatgct gatgatttgc agcatttaca
     1201 cacaggtaca gcgctccccc aggacagaca aaactttttg taataagttc tcctgaggca
     1261 ctcccgcttg atagagagac aaaaccatct tctggcattg tggcaatacc tatgattccg
     1321 tgcggcagcg aaacagaatc tggcagccca tgacagaggt ttcgcgcttc atagaaaaat
     1381 agcaaatgat tatttatttt taaaggagga tttgaagcca gcccaatcca tctggagtca
     1441 atggattttt caaaatttga ttccataaaa gcacatcgca atctatttct ttgccatacg
     1501 atactatccc gactgctaat cagttcaata tccaggcggt cttcaacaga atggtatcgc
     1561 tgaagaaacc caagatacat tgaatggtat ctgaaagcaa ccaggcagta aaattgtaaa
     1621 tcaaccggat catcaaggtc gggttttaaa atcatttctg atggtgtcca tctgacaaaa
     1681 tctttgctat caagacgtac aataattctt ttgccaaatt ttttcatcat atccgggggt
     1741 ctggtaaaaa ttacatacgg tttttcaagg gatgaatcaa acatcagagt atttcggtct
     1801 ccatagccga acaaaatcgg gccattccag ccctgccatt tgattccgtc agcagagaaa
     1861 tttgcccaga tgtgatttct accatgatgg tcagttctgc aggaaatcat tttgtaacgg
     1921 cgactctttt ttttgtccac agtatcataa ataacactgg ctgcatcagg atgatgagga
     1981 tgttctgctg gaatgacgat attgttattt gttgaaccct taaaatcaat caatcccagt
     2041 tcaggtttgg accagtgaaa tccatccgaa ctctctgcat aaaggatatc gcgaccaaga
     2101 tttctttttc cacagaacga agaataccac attttaaaaa ttttcatcac agggtctttc
     2161 atcacagaac cataaagaat gaggcaactc tgttcccatg gtttatctgg tttcagaata
     2221 acgccaatcc gttcgggtga ccagtatttt ctctgaagat tgtttgagta ttcaataatc
     2281 gcatcatcca caaacaactg gtgacaggct gaattagtcc tcatttttcg tttttctttc
     2341 caaacatcat caattggtca attattccat ttgccacaga gtcagtattc gcatatcttg
     2401 agggtccaaa gaaaatggtt tttcccaggg ttattgttga tgtgccagat tttatccatc
     2461 ccttcgagac ctgctggtct attttaagca cagtctcgcc ttcaatatcc cagtccacaa
     2521 tgatatgatg ccattctccc tgtttccagt tattaacagg aatgctgagc aacaatccat
     2581 tttgccagtc gtcggtgaat atacgaaagc agatagttcc atattcggtc ttcataatct
     2641 gaaaatggtt ttcatcgtcc accatcaaaa accgtgtctt tttttccacg ccatcccagt
     2701 atgggttgaa ccagaaatca atgatacctc tttttgatgt caggagattt ttagtttcaa
     2761 atgccagaac tgcatctttg tcaagcacaa taccttttcc tgtttttcct tcaacaaact
     2821 taatgttttt tgcaataacc gggttgtgtc cacaagtatc agtgagagta tcttcaaatt
     2881 ctatcgcgag ggttttcatc atattttctt cagcaatagc gatactgcag aaacaaatca
     2941 gtatacccag tgcaaaaacc atgtttttca taacgcctcc tttttttact gaaacccatt
     3001 aagaacacag aatgcaaaat cttttttcat ccgggagatt tttcctggtt taacttctac
     3061 agtagcaagg atttttcctg aaggtaaatt ttttgggtct atccttataa tgaaatcacg
     3121 caaggaatat tcatcaacag gaatctgttt tctgtagaaa atttttttct cttcattgtc
     3181 tgcaagtgtc agagtaattg gagatgaccc tgacagtttt ttgtcaatac tgatatgaca
     3241 gaatacctgt aaaacatcaa taccctttat tagatagtaa gtatcagggt aaatctgaaa
     3301 aatctgatta ctgtctatcc gaagggtttt gctatcatat aaagtattct gtatctgatc
     3361 aagcaatttg agttgaagtt caaaagattc atctgtttcc agattgaatt catacgggac
     3421 acaggtcatt gaaaatcggg atacagacac atttctggtt gtttgttgaa cagggatgcc
     3481 atgttgattg taaagtgtta cgacaatggt aacatttttg tccttatcac aacgattact
     3541 aatttcaata cttccctgat tttgtccgat attcgactcc ggtagtaaaa cttttgaaaa
     3601 ataacaatca ctgtcagaaa tatttaatct gccaaagtca gccgtgtaag caaatcctct
     3661 tcctgatgtg gaccagcagg taagttcacc cgtctgtttt tcttcacggc acatattaat
     3721 tccagcgata gacgcttttg aaagtccaac atctttacga ggaaatttga gttcaacctg
     3781 ccagtaaaaa gggaattttt ttacagccgc ttcaacattt tctgagttcc attttggttt
     3841 gaattctcca gacaaataaa aagcgtcagc ggttgcgccg ttggcattca ctacaaactg
     3901 atagaaattc tgacatccag tatcattacc aataaaaacc tccacacaat catcattcca
     3961 cacttcgcca tcccgatttg taacgtgtgc gacaaggtta ttcatggctg gttcaaaatt
     4021 cttgaaggca aagtaaagat tttcattgtc tgcggcaacc atcacttcag tttttttcct
     4081 gggtattgag ccatcggtac ggttgacaag gtgcagtact tttgcatgtt tccagcattc
     4141 ttcagtaaaa tcaccgtcaa taaccactgg ctgtgttact ttttctgctc cagtaatttc
     4201 ttctttccag tatgtttttt gtatcctcgc atcagtttgt tttaaaactt caattactgg
     4261 tttcattttt tttgcagaca aactggtttt tccttccaga gcccggtcaa ttgcaataat
     4321 atgattggca acagcccacc gaattttctg acagtacata tccatccatt cttcacagga
     4381 aagaggttca ccggattttt tgatgtaacc tgcttcagaa atcataccag aagcaaattg
     4441 attaagggta ggtacaattg atttaatgtc agcaatttcc tgttctgcct gtcttgctgc
     4501 tttttctgca acaggcacat ttgatgtttt tcctttctgt attttgtttt cgagtaatgt
     4561 catgtatctg cagtcatcaa atccttctct ccagttttcc cacctgagtg tggggatatt
     4621 ctcgtctgca acaggatatg taaggaaaaa gtcagacata tttccatcaa ggtctgtatc
     4681 aggactacca acgatgttgt tgtatctcca tggtgccacg ccaacaagtt gagaaaacca
     4741 gaagccaaat cccaccagga aacgaaccga tgaataactt ccagaagctg ctgccacgtc
     4801 gtttggataa aaccagtttc ctttctgtaa ctctggttgg tcaggtacag actcaggacc
     4861 tgaaaacttt attacatcaa taagtttaac aagatcctcg ttgagatatc cctcgcccaa
     4921 tctacctgta acaagcgttc tggcaccagg aacctggtgg acaagattac agagacgtct
     4981 ggcaatttcc tgtctttctg gatcacacgg ttcatcaacc acgtaaaaaa gaatttccgg
     5041 ccattttctc tcctgtgcag tttgctggat gaacttaatg ccgttgacat aaatacggtt
     5101 aaattcgttt gagaacggtg gaggtatttc ttgactgccc cgaggatggg gtacagggtc
     5161 tcctgtgata gcacagatat cctctatcat aggggtgata ccaaaaaaac tatgtttgat
     5221 gaccccggat tgtatcattt tttcgatcaa ttctactgga agagtgtact caatttgatc
     5281 accctttttt gagattcgca ggcgaaaacc agggttgcaa atagatgata taccatgttt
     5341 tttcatatcc acaaggtctc gttgtaacag ttcctctaac cttgataagg tctggcgttc
     5401 tctataaagg cgattgatgt cagggtgtga aagaatataa agggggtcaa gataatgaaa
     5461 accaaatatt ttcctggatt cttcaggcag attgaatggc aataccacaa acataattgg
     5521 cacctttact gttctgaact cagatgtttc gaaaatgact gagcccctgt ataatcctgg
     5581 ttccgatttt tctggaacat tcactgttaa ccagaaaaat tttgtttcat caattcgcag
     5641 tggcgctttg aatttttcaa gtaattcagg tacccattca tattccttac gtgttgccca
     5701 cagagttctg tgtgctctgt atgttgcaac gcgaagggtt aaaaaagagt ttggtatttt
     5761 tgtgcgacta tcttttttta aatctgtaat gctggctgaa acaattgaaa catcattcct
     5821 gagtggacac agaacgaaac atacaggttc aagttcaccg ggtgtcccaa aactccagat
     5881 tggaaaattt tgtcgttcct gggctggtac actgttggga taaactggaa gatatgaagg
     5941 ccagaaagat acaataaagc cattttgtat ctcctttgaa ttttttggac gtgcatgttg
     6001 tgaagagtgt gttttttccg accacgagac aagttgctcc ttaggagcaa gtataagttc
     6061 tttttctttt ttctcaatga tcggggctgc tttataccac agattttgtg gtgccagaat
     6121 aataccgcag acaacaaaat gaggatttcc tttaaattca aaatccagat tattttccct
     6181 tgcgtctata tcaaacagcc aggtttgaac tcgtggaata tagttggttg tttctttttg
     6241 cgagattacg gtctttccat tcacgatgac tgaaaacgct ggtggaatat atgtcatatc
     6301 gcctgccacc agccagcacc tgtatttccc gggggttaaa gaaacacgaa atgtattttc
     6361 acctgtgcct tcgtgaaaac tgtattcaag tttgtctttt ctgaattcat caggtactct
     6421 tctggaagtt gccagaattc cttttttaat ccacccatat ccattttcaa tgctccataa
     6481 acgcgctgga gaaaccctga caaaaccagg ttctggctct tctgtttgta aaccaaagtc
     6541 aagacaatag attgaagaat ccagggtggt tttttgggag taagcaacag gaaggttatt
     6601 aagaataata acgaatgtaa aaaaaacaaa acatttcaaa gtccgaccat tcacaagcat
     6661 gccgttatcc tgtaattctg ggctgaaagt tcttatcatt taaatgtttc ctgaaccctc
     6721 taccattatt ttaccttttt atttccagtt ttcaattttt tctctcccgg cgatatacaa
     6781 tataatatca acaaaaacag gagaaaaata tgaacaaaga aatatttcaa aaatatgagc
     6841 agcaatacat ggcatcgttg cttcatgatt ctattccctt ctgcatgaaa catgctcctg
     6901 atggaaaata tgggggatat tttcagtgcc ttgattttga tggttctgtg gtcagtactg
     6961 ataagtttat gtggtctcag aatcgagttg tctggatttt ttcgatgctt tacaacaatc
     7021 ttgataaaaa accagagtgg ctggagatgg cacaactggg tattgacttt ttgaaaaagt
     7081 atggaaggga tgaaaatggt gattggtttt ttgctcttga cagaaaagga aagccaattg
     7141 tcagggcata caacatcttt tcggattttt ttgcagtgat ggcttttgcc gaatatgcaa
     7201 gggcttctgg cgataaagag tgcgcagaaa ttgcggcgaa gacatatcgc agaatacagt
     7261 tgagaaaaga taatcccaaa ggtcagtaca ataagataat aaccgaaaat agaaatctaa
     7321 aaaaccttgc ttttccgatg attaatctta atgtggcgac tattcttgct aacgccaatc
     7381 acgacggagc atataataaa cttattacag aatgcgtgaa cgaaataatg aaccagttcc
     7441 ttgaccgaaa gcaccgtgtt gtcagagaaa atatcgctgt tgacgatact tctgtggatg
     7501 agatttatga aggaagacat gttaatccag gtcatggaat tgaggcaatg tggtttgtca
     7561 tgcaggccgc aagaaagaac aatgaccagg caacaattcg tgaagcatgc gaggcggtaa
     7621 aatggctact tgaatttggc tgggatgaac aatatggtgg aatattttat tttctggacc
     7681 tgtacgggaa gccacatatt gaactatgct gggacatgaa actggagtgg gttcatgtag
     7741 aagcgctggt agcaaccctg atgggttatt atcttacagg agactccgaa ctgtttgatt
     7801 ggtttgaaaa aattcacgat tatacatggc agcatttccc tgacagagaa tatggtgaat
     7861 ggtataaata tttgaatcgc aggggtgagg ttaataacag gtgtaagagc aacaaatggc
     7921 agaacttttt ccacctgccg cgggcactct atttgattgc aaagatatgc agggctctgc
     7981 aagatacaga cctttaaaaa ccttaacctg ttgcctgttt tcagttaacc taggttgctt
     8041 tttggcttct gggatacaat gtgatctata agcgaaacag cgttgacaac tggaacatct
     8101 ggaagaatcg gttttgccgg tgctatgata tagccaccat ctttacccat gatgtcaagg
     8161 caccggttta cttcgtccct tatctgttgt ggtgtaccaa agggtagtgt tttctgcgta
     8221 ctgataccac cggcgaaaca caaatccctg ccaaaatgtt ttttgagcgc gaaaatatcc
     8281 atagtttctg gctggatggg ctggagtata tccacacctg cttcaatcag gtcgggtatg
     8341 atcgggctga tatgcccgca ggaatgcagg taaacttttt ttcctgcttt tttaattctg
     8401 gcatacattt tctttattcg tggtttgata aacctgcgcc acattgccgg acttaagagc
     8461 atcgcatgtt ctccaccata atcgtcgctg aatcctatgg catcgattga atttccataa
     8521 tgctcaatga gtttgtcaat aatttgaatc gatatttctg caaggtgatc aagtaattct
     8581 tcaacaaatt cgggattttc acaaaaatcc atcataattt cctggaagcc acgcattgcc
     8641 catgttcgtt cgaaaaataa taaacccagc tggactattt tgaatctgtc attattttct
     8701 gtcagccatt tgtcaagatg ggcataatgg gaaaaggttg ttaggtcagg aaacctatac
     8761 cctgataggg acggttcagt taaagcgggt tttacgatat gcaaaatact tcctttctgc
     8821 cagatggttc caaattcatc cctggacgta ccatcaggca gcatacattg ttcggcagta
     8881 atttctatca tcacagtgtg gtcgtaaatg taattctttt tcaattcagg atttctatag
     8941 tgatttgtca accgttcaag catcgcaggg tcaatccaga tataataggg acaaatatct
     9001 ggttccctga aagagagtgc agtgaatact cttttacgga aaattgactc tgacgcattc
     9061 atatacagtg aattcagtta tgctattttc ggcattcaca tcataccact gccgaaatgt
     9121 cctgtcaaag tgttgtgaaa tgtgtaattt ccctgttgtt ttcacagaat accacacgca
     9181 ctatcgaccc tggtgttgtc ttgacaattc tggagaaaat gtattatgct aatgtgtaac
     9241 cgaaagactt aggaaagatt ttccattaca atgaaacaga aactttcttt taatacaaaa
     9301 gaataccagc aattaatttc cattttcaat tctcttgacg aaatgattta tgtcacagac
     9361 cctgaaactt atgaaatcct ttatattaat cctgttttga agaagacctt tggagatgta
     9421 ataggtaaaa aatgctacag ggtatttcaa aataagaaca aaccatgcag tttttgcacc
     9481 aataaattta ttttcggacc agaggcaaaa ccggtttata tctgggaatt tcagaacaaa
     9541 gtaaataagc gctggtatcg ttgtattgac agaccgatag aatggataga tggaaggaaa
     9601 gttcgttttg aacttgcgat agatattacc gataaaaaac tgatggaaga attcttactg
     9661 gaaagccagc agagatacag agacctctgg gataatgcgc cgattgctta tcacattgtt
     9721 gataaaaaag gaataataag ggatgtcaat aaaactgaac tcaaactgct gggatattct
     9781 aaaaaagaga tgctgggcaa accgattttt gattttatta ttccatcgca aagggaagaa
     9841 gcgcgagaaa ggtttatgct gaaaatgaaa gggaaaaaag tcgacaaaaa atacgataga
     9901 atttatatga aaaaaaatgg cacttctttg tatgtttcca tacatgatgt tcctgaaaag
     9961 aatgaaaaag gtgaaattat tggtatgcga acagccatga tagacattac agaattaaag
    10021 aaaatggaag aaaacctgaa agaaatatcc cttaaagacg aacttactga agtttataat
    10081 agacgaggct tttttacact tgcagaacag gaaattcgta gaacccagag gggtaaaaat
    10141 ccattttaca tacttttcat tgactttgac aatctgaaaa gtatcaatga caaaaaagga
    10201 catctgtgtg gtgatgaggt tttgaaaaaa cttggtgaaa ttctgaagga tgtattcaga
    10261 caatccgata ttatagcaag aattggagga gatgagtttg tggtccttgc atcagatgtg
    10321 aaaggtagta aagaggtcaa agtgttgatt gatagaatga gaaagacaat tcagacattt
    10381 aataagggaa acaataaata tcagatttca atcagtattg gcactgccag atatgatggt
    10441 actaagcctg ttacaattga cggattactt gttgaagcag acaggaaaat gtatcaggaa
    10501 aagattgcaa aatacaaact tgcaaaatat caatcgcgaa ccatttttga tctgtaatag
    10561 ggttgagaat ttctttcgtg gcttctgtca ttgttcattt caaatcagcg tttgaaattt
    10621 ttagaaaaaa tgataaacta atttcagcca atcaagcctg tggtatttgt aaaatataat
    10681 atgtttgaaa acgatatcag ccgccttagc tcagtggtag agcagggcac ttgtaatgct
    10741 caggtccgcg gttcaagtcc gcgaggcggc tctgaaaaat tttctgaatc tgttgccatc
    10801 catctacact cctgccctat acggcaacag atttttgcag gtatcaatga aaaatatcct
    10861 gcatccatca gcacagtgat tcaaaaaaaa taatggaggg cagatggaaa caattgtgac
    10921 aaagaaagat attgtttcag gattgaaaaa actgggactc aaaaaaggag atattgtcct
    10981 tgttcacagt tcattgagca gtctgggtaa ggttgaagga ggtccagaaa cagtgataca
    11041 ggcgctcatt gaagtggttg gaaggacagg tacaatcatg atgccatatc cactgggcaa
    11101 tgcgacaatc gctaaagtat tcagttcaat gaaaggcgtg ataaaaagta tacatcctgt
    11161 acactctgtt tctgcaatgg gagcaagggc aagatattta acaaaggacc atgataaaat
    11221 gcctactgcc tgtggaaaag ggacaccatt tggaaaactt gttgacctcg gtggatacat
    11281 tttgctgatg ggagttgacc aggacaggaa tacaaccctt catactgctg aagattatgc
    11341 ggatttacca tatctttcag aaaaggaatt taagtatgtg gatgaaaacg gtgttgaaaa
    11401 gacgattgtt ctaaaaaggt ttccaggacc tcacagaaat tttatcggta tggacaggat
    11461 ttttcttgaa aagaaaatca tgaacataga aagaatcgga aacgcaatgg tgcgtcttat
    11521 tgatgcgaag aaaatgataa gggtgtgtct cgacgaattg aaaaaaaatc ctgccgcatt
    11581 tctgtgtgaa aatccaaact gtgctgactg cgtgatgcag agaggaaaaa tcaaagaggc
    11641 aagattgaaa aaagagtgtt tcactctttc ggcaacaacc agccatattg cttcagaacc
    11701 cgaagagatg ttaaaaatcc ttcagggaca gggagtaaaa acaatagaac ttgatgttat
    11761 atggaataaa ccagtaacaa cacttgctcc atccgaactt cagtatttaa aggatttgtt
    11821 tgcaaagcat aatttttctg ttgcaggcat atcctgtaaa gaagattgtg atattgaaaa
    11881 gtgtcttgat atcgctgaat ttttcggtgc aaagtacact gtgttgccaa atcttatctt
    11941 ctctcaggat attcaaaaac agaaacgaca atttattgag tcactttttc ctgtgattga
    12001 aattgcagag aaaaaaaaca ttgtgctttt gatagaaaac aggccggcta ctgtgtgcgc
    12061 caggtcttct gattgtcttg aaattctggg aaagattaaa tctcctcatt tgaaactggc
    12121 attcaatcct gcaaactttg caattgctgg agaaaaacca tttctgggaa tatctcctca
    12181 tgttagaagg atggcatttg ttctttatgt caatgatgca ttatttacag ggaaaccaca
    12241 gtttcccgga tatggcaatg ctgagataaa agaattgatg tcaattttgc ggtgctggtg
    12301 tttttcagga tacttttgct taaaaccagg aattggaact ggaaaggaat ttttcaattc
    12361 tgcagcggat gcattctggc atttgcttga aacaatgtga aaacatgaaa atcatagacc
    12421 tgacacggga aatatcgaag gatttcagaa cagatattgt gaatattcgt ctatctggga
    12481 cagaatatca cgcatttgtt catcaattcc atctttcttc tatggcaggg acttacattg
    12541 attttccagg ccacatcaaa gagtttgata atgggtttga ttcatcaaat tatccagtgg
    12601 aaaaactttt tatggtggat gccactgtga taagactcaa tcgctcaggc aaacagaggg
    12661 aaatacatgc agaagaactt gatgcagcaa atgttgtact tgatacgccg gctgtgattg
    12721 ttgatgcagg atggaattta ctcaaagaaa aaaatcagga tgaaatatat ttttttggga
    12781 aggatgccat ttcctggttc atctcaaaaa acatatacct ttttgtttca gatgtgtacg
    12841 aaaatcacaa agacccacga ggtatctttg ttgaattttt tagcaaagga attctgactg
    12901 tttgtaatcc tgtaaatctt gaaaagattg cagcgccgaa agtcaaactc tgtgtttttc
    12961 cgctcagaat acccggtgta acccaggttc catgtcgtgt tatcgcagtg atgtgatgta
    13021 ttattgtggc cagcccgatg ccacccacct ttcactgcca agtgcttcag cgtgcccatc
    13081 cacaaacacg acagtgacca ttttgatgta ccagtgtctt ccacttactc cggtataata
    13141 tccatctttt tattacttct ttgacaaatt tcatgggttt ctggtgatac
//
LOCUS       DAORYU010000011        12852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_3824_length_12852_cov_4.988904, whole genome shotgun sequence.
ACCESSION   DAORYU010000011 DAORYU010000000
VERSION     DAORYU010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12852
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3824_length_12852_cov_4.988904"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1146)
                     /gene="dnaB"
                     /locus_tag="PLW07_00810"
     CDS             complement(<1..1146)
                     /gene="dnaB"
                     /locus_tag="PLW07_00810"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOL49097.1"
                     /translation="MANKKNLPVNEQAPPQNIEAEKALLASMLLEEDALMRGLEIIRK
                     EFFYDNRHQLIFSAIQDLFEKNIKCNIITLTNYFKETNKIDLVGGISYLTSLFDFVPT
                     AAHIDDYAKIVREKFILRSLINVSTRIVSEAYACNQDVDSLLDSAEAMIFDVSEHMNA
                     REAYPISMLVKENLDLFEKIHTHQGHVTGLPSGYIDLDNITSGFQRSDFIIIAARPSM
                     GKTAFACNIAQNLAVTQNIPVLIFSLEMSKEQLVQRMLCSEARIDSSSMRKGILSDRE
                     MGKLIQAAGILEKAPIFIDDTPALSIFEIRARARRLKSRENIQLVVVDYLQLMRGRGR
                     YENRQQDITDISASLKAMAKELSIPVIALSQMNRLIERREDKRPILAD"
     gene            complement(1210..1668)
                     /gene="rplI"
                     /locus_tag="PLW07_00815"
     CDS             complement(1210..1668)
                     /gene="rplI"
                     /locus_tag="PLW07_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOL49098.1"
                     /translation="MKIILIKDIKGLGQEGDVCEVKDGYARNYLIPRGLAIEANEKNI
                     KKVEEIKRLVALKREKLLKEAEALQKKLNKISITIEAHAGEEEKLFGSVTAEDIAISL
                     KNQHDVEIDRHQIHLEEPIKKLGIYKIPVHLIGEINAELKVWIVEKQETS"
     gene            complement(1680..1937)
                     /gene="rpsR"
                     /locus_tag="PLW07_00820"
     CDS             complement(1680..1937)
                     /gene="rpsR"
                     /locus_tag="PLW07_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOL49099.1"
                     /translation="MEQKTRDSRKKRTPRKKKRAKGCKFCEDKVHDIDYKDIGRIRRY
                     MSGRGKILSSKLTGVCARHQRALTKAIKRARYMALLPYIQV"
     gene            complement(1867..2241)
                     /gene="ssb"
                     /locus_tag="PLW07_00825"
     CDS             complement(1867..2241)
                     /gene="ssb"
                     /locus_tag="PLW07_00825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOL49100.1"
                     /translation="MRYTPSGSAVTSFGLAVNREYTTKDGDKREEPCFVNLVVWGKRA
                     EICYEYLKKGSLIFVEGRLNYRQWETSDKEKRSTLEVNVENFQFLDKSLPEIKGEMVN
                     GTKNEGFKEETDTQEKETGEGL"
     gene            complement(2282..2608)
                     /gene="rpsF"
                     /locus_tag="PLW07_00830"
     CDS             complement(2282..2608)
                     /gene="rpsF"
                     /locus_tag="PLW07_00830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOL49101.1"
                     /translation="MPRKYEACTLLDPELDQNGLEAETSAILQVISSSGANVVHSELW
                     GRRGLMYPIKKKKEGYYYLFYFECNPQDLRKMEEGLKHREKILRILVIAKKEFPEFVK
                     NGKSES"
     gene            2759..4204
                     /gene="gltX"
                     /locus_tag="PLW07_00835"
     CDS             2759..4204
                     /gene="gltX"
                     /locus_tag="PLW07_00835"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOL49102.1"
                     /translation="MIRVRFAPSPTGFLHIGNARTALFNYLFARKNNGRFILRIEDTD
                     TERSKQEFEESIIEDLKWLGLTWDEGPDCGGSFGPYRQTERLHLYREYANKLLSEGKA
                     YRCWCTKEEIENRVMPGSPDRAAGYDNYCRNLTESQIKEFQTQGRKPVLRFKVPHKEI
                     VVNDIIRGIVKFEPGSLNDFIIMKSNDIPTFHFAVVIDDLHMQITHVVRGEDHLSNTP
                     KHILIFEALGHTIPMFAHMSMTLGPDRTRLSKRHGATSVRQYREKGFLSDAMFNYLAL
                     LGWSSKNNQEIFSRQELIQEFSLEACNLSNAIFDPAKLLWINSQYLRMKPAKEILDLS
                     IPFLEKAGYHESDLQQHRDIIEKALEMEKEKLKTLEDVAERTGYFITEDISWDVELTK
                     KFKTDQTSRILNALKSNLERLEPFEKENIEDMIRKFCQENNLKTGEVFHPLRFALTGT
                     TKGPGLFELIELLGKQRAVKRIGGFLTYITR"
     gene            4201..4737
                     /locus_tag="PLW07_00840"
     CDS             4201..4737
                     /locus_tag="PLW07_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HOL49103.1"
                     /translation="MISKAKTLELKKAATEILKKACIAVRDEEIASMEIVELGLGKPE
                     IIGLQIIVYVNNDRYCAKELILLPGQTCPEHKHGPLGPNNPGKMETFRCRWGEVYLMV
                     PGQQTTPMKEKVPDFRKEYFTVFHEIVLKPGDQYTLLPDTLHWFQAGNQGAIISEFSS
                     PSFDEKDIFTDPLIKRKP"
     gene            4734..6134
                     /gene="cysS"
                     /locus_tag="PLW07_00845"
     CDS             4734..6134
                     /gene="cysS"
                     /locus_tag="PLW07_00845"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012671029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOL49104.1"
                     /translation="MTRILFFNTLSRQKEEFKPIEDGVIKMYTCGPTVYDYAHIGNFR
                     AYVFEDILHRFLEYAGYRVIRVMNITDIDDKTIAGAIKEGVSLDQYTERYIKAFFEDM
                     ECLKLKKATYFPRATQHIPEMVSLIKQLIEKGHAYEKNGSIYYKISTFPNYGKLSHYD
                     FENLLTSSTDADEYEKDEVRDFALWKATKNEPFFWDTEIGRGRPGWHIECSAMSMKYL
                     GETFDIHTGGVDNIFPHHENEIAQSEAATGKKFVNYWLHCAHLLVNGEKMSKSKGNFY
                     TLRDLLNKGYHPSEIRYLLMTTHYRDPLNFTEQSLKQAEKTIKHVQAFYQRISFALEN
                     EEHSRQVHQDIEKYINEASEGFVGALSDDLNISLAMSEVFKFMHGINEICEKNKITGG
                     DAKKIIEFFDRINEVIAIIEPVEKLPEEIVNLIKKREQARKEKNFTLADQIRNTLAES
                     GIVLEDTPYGTRWIRK"
     gene            6119..8950
                     /gene="uvrA"
                     /locus_tag="PLW07_00850"
     CDS             6119..8950
                     /gene="uvrA"
                     /locus_tag="PLW07_00850"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOL49105.1"
                     /translation="MDQEIKNQNMIPEESAHYIIVQGAREHNLKNISVKIPRNKFIVI
                     TGISGSGKSSLAFDTLYAEGQRRYIESLSAYARQFLEQMKKPDVDHIFGLPPAIAIEQ
                     RKSASNPRSTVATTTEIYDYLRLLYARIGQPHCPECRRPISSISSSEIIDKIMTLPEK
                     TKLLMLAPIIRGRKGEYRKFLEEIRKSGFLKVRIDGKIFDLDDEISLKRYKTHSIEVV
                     IDELEKSSDRQRIAESVEMGLKIGKGLLIVNQNGSDQLFNEKFGCPVCGISFDQLEPR
                     NFSFNSPYGACSECKGLGFLMKIDPELVIPDRNKTFREGAIKAWQDTGGRHIFFYYRG
                     LLRSMLRHIGKTLDTRVCDLSDREINFILYGDEEEGYEGVIPNLERLFQQTESEFRRE
                     EIMKYMRELVCPSCNGDRLKPEVLAVTVENKSIMDVCRMSVSSVKRFFENLNLMEKQQ
                     MIARQILKEIISRTSFLEEVGLDYLTLDRMTHTLSGGEAERIRLATQIGSGLVGVLYI
                     LDEPSIGLHPRDNSRLITTLKHLSSLGNTVIVIEHDEEMMRNADYIIDLGPGAGKNGG
                     EIVGCGTIEEIMLSERSITGKYLAGMLSIPVPKTRRKPQDKYISIIGASHNNLKNINV
                     DIPLGLFVCVTGVSGSGKSSLIDDVLTQGLRKILYDAKTLPGKHKAIVGIENTDKVVI
                     IDQSPIGRTPRSNPATYTGIFDLIRKLFSYTQESRIRGYKPGRFSFNLRDGRCQACQG
                     EGIMKIEMHFLPDVFIPCDVCGGKRYNRETLEITYRGKNIAEILAMRIEEALDFFKHI
                     HPISTKLQTLCDVGLGYLELGQPATTLSGGEAQRVKLAAELSRKGNEKTLYILDEPTT
                     GLHLYDIQKLLVVLNRLVDKGSTVVVIEHNIEVIKSADYIIDLGPEGGDRGGQVVCTG
                     TPEEVAKNKKSYTGQFLNRVLYQKITTVA"
     gene            8961..9938
                     /locus_tag="PLW07_00855"
     CDS             8961..9938
                     /locus_tag="PLW07_00855"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOL49106.1"
                     /translation="MVANLPPHYHTKEAELKNAKTPEEKIAILLEMMAIMPKHKGTEK
                     LQKELKSKIAKLRKEASEKKVISRGSPVPTIEREGAGQVIIAGPPNTGKSSLLAAITK
                     AKPEIADYPFTTKIPQPGMLQYQDIQIQVIDSPALSSGISENWLGDMMRKSDVILLLL
                     DLSDDDILEKMDETMQVLEQFNIKTPGTWEFTKKILWAGNKIDIPSWKDIKNVLSELY
                     PIGDHIIPVSVLQKINLDLLAEQIFIKLDIIRVYTKIPGKPPEMKDPYTIAANSTLED
                     LAKSIHKDLVKTFKYARLWKQGTGNPVIAGRDYVLEDRDLVEIHAEEMK"
     gene            9935..11002
                     /locus_tag="PLW07_00860"
     CDS             9935..11002
                     /locus_tag="PLW07_00860"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOL49107.1"
                     /translation="MKVKLAAENIIKKYNETTILDSITIRVDRSEFFALLGPSGSGKT
                     TLLRIIAGFVVPDGGKILIEEKDITYIPASKRNMAMVFQNYSLWPHMTVFENVAFGLR
                     IRKIPVNDIQEKVNRVLDMVDLQQHSTKKPQQLSGGQQQRVALARALVVEPDILLLDE
                     PLSNLDAHLRDQLRQEIKTLQKNLGITTIYVTHDQKEAMYLADTIAVMLDGKIVETGR
                     PETIYQKPSSLQTARFLGQLNEIRGVVKKIKNGSYYIETDIGMLESKSEHEFSVNQQV
                     IAGFRPEFIQFPAHQDTATKIECKLHSHEYAGTFINALLKKNNILFKATLSPVSFNQQ
                     KDRIYVIGIDPENILLFKIEQ"
     gene            10999..12639
                     /locus_tag="PLW07_00865"
     CDS             10999..12639
                     /locus_tag="PLW07_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOL49108.1"
                     /translation="MKNLFFALLLFFIIFTGYPLVFVFWKSADVSIHLFPVVWSNISV
                     RQSFINSIFLAISVTIATSIIGFPLAYVMSFVKIPMKNFWRKAFLLPLVAPPFVSAIA
                     MRQILSRFGPVNLVLMKLGITTTPVDFLGTGLTGIFILQVLHLYPIIYLNILSSLENI
                     EGSCIEAAENLGASVFQNFRKIIFPLSLPGYFAGAFLVLIWSLTDLGTPIVFDYPNLI
                     PVLIFQSLTDIYSNPAGYVLIIFICILAMILFFFTRYLLGKMSFVGIARYKTTMDPLH
                     VPASVSIAISVFLGFLVFVSLLPHISVILNSISQKWFFTILPDRWTIQYYRDVFLHPI
                     ARISLINSTVYCIITGLLIMIISVATGLILSRSQIKGKNILESFVILPMAVPGIVFAF
                     SYVLAFSETIFDPRNFPMFLLIVAYVLRRLPFAVRAVVANFQQIDISCEEAAFNLGAS
                     RWKTFVSITMPMLRNGIIAGFIFSFAFSMMEVSSSLILVNKQQYFPIAKGIYQLAGRI
                     TDGPYIASALGVLGMIISFAALMIIQRISGKRDISFSI"
     gene            12662..12734
                     /locus_tag="PLW07_00870"
     tRNA            12662..12734
                     /locus_tag="PLW07_00870"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12695..12697,aa:Val,seq:cac)
BASE COUNT         4118 a         2556 c         2523 g         3655 t
ORIGIN      
        1 atcagcaaga atcggccgtt tatcttctct tctttctatt aatctattca tctgtgaaag
       61 cgctatcaca ggaattgaaa gttcctttgc cattgctttc aatgacgctg atatatctgt
      121 gatgtcctgc tgcctgtttt cgtatcttcc ccttcccctc attaactgaa gataatcaac
      181 aaccacaagt tgtatgtttt ctcttgattt caatctccgt gctcttgctc ttatttcaaa
      241 tatggaaagc gcaggagtat catcaataaa aattggtgct ttttcaagaa ttcctgctgc
      301 ctgaatcagt ttacccattt cacgatctga aagaattcct tttctcatgg aagaagaatc
      361 aatacgggct tcagaacaca gcattctctg aacaagttgt tcttttgaca tttcaagact
      421 gaaaatcaac accggtatat tttgagtaac cgcaagattc tgggcaatat tgcaggcaaa
      481 agcagttttt cccatggaag gtctggctgc aatgataata aaatcagatc tctgaaaacc
      541 tgatgtaata ttgtcaagat ctatgtatcc gcttggcaat cctgtcacat gaccctggtg
      601 tgtgtgaatt ttctcaaaaa gatcaagatt ttcttttaca agcattgata tcgggtatgc
      661 ttcccttgcg ttcatatgtt ctgaaacatc aaaaatcatg gcttcagcac tatcgagtaa
      721 actgtcgaca tcctggttac aggcatatgc ttctgaaaca attctggtgg aaacattaat
      781 cagggaacga agtatgaact tttctcgaac aatctttgca tagtcatcta tgtgggccgc
      841 agtcggaaca aaatcaaaaa gggatgtcag gtatgatatt ccacctacca ggtcaatctt
      901 gtttgtctcc ttgaaataat tcgtcagtgt gataatgttg catttgatat ttttttcaaa
      961 aaggtcctgg atagcactga aaattaactg atgacggttg tcataaaaaa actcttttct
     1021 aataatttcc agtccccgca tcagagcatc ctcttcaaga agcatcgatg caagaagagc
     1081 tttttctgcc tcaatatttt gaggtggagc ctgttcgttg acgggcaagt tctttttgtt
     1141 agccatgatt cctaccagca ttaccacggg gcaaattcgc atcaaccggt ttgataaccg
     1201 tttattatct tacgaagttt cctgcttctc tacgatccag accttcaatt ccgcgttaat
     1261 ctctccaatg aggtgtaccg gaatcttata aatgccaagt tttttgatgg gttcttcaag
     1321 atgaatttga tgcctgtcta tctcaacatc atgctggttt tttaaagaaa tcgctatatc
     1381 ctcagcagta acagaaccaa acaatttttc ttcttcacca gcgtgggctt caatggtaat
     1441 tgatatcttg ttcaattttt tctgaagcgc ctctgcttcc ttcaataact tttccctttt
     1501 tagcgccact agtcgtttaa tttcttcaac cttcttgata tttttttcat ttgcctcaat
     1561 cgccaaacct ctcggaataa gatagtttct tgcgtatccg tccttcactt cgcatacatc
     1621 cccttcctgt ccgagaccct tgatatcctt tataagaata atcttcattg ttctgctcct
     1681 taaacctgaa tatagggaag aagagccata tacctggcgc gtttaattgc ttttgtaagg
     1741 gctctttgat gccgcgcaca aacaccggtc agttttgaag acagaatctt gcctcgtccg
     1801 ctcatatacc ttctgatccg accgatatcc ttgtaatcaa tgtcgtgtac tttgtcttcg
     1861 cagaacttac aacccttcgc ccgtttcttt ttcctgggtg tccgtttctt ccttgaatcc
     1921 ctcgtttttt gttccattga ccatctctcc ttttatctcg ggtaatgatt tatcaagaaa
     1981 ttgaaagttt tcaacattta cttccagcgt ggacctcttt tctttatcag aagtttccca
     2041 ctgacgataa ttcaaccggc cttccacaaa aatcagtgaa ccctttttta aatactcata
     2101 gcatatttct gcccttttcc cccagaccac aagattcaca aagcatggtt cttctctttt
     2161 atccccgtct tttgttgtat attctctgtt cactgccagg ccaaaactgg tcacagcact
     2221 gcctgagggg gtatatcgca tctcaggatc ccttgtcagc cgtccaatca gaaaaacttt
     2281 attaagactc ggatttgcca tttttcacaa attcaggaaa ttcttttttt gcaatcacaa
     2341 ggattctgag aattttttct ctgtgtttga gcccctcctc catttttctt aaatcctgtg
     2401 gattgcactc aaaataaaaa agataatagt atccttcttt tttcttttta atcggataca
     2461 tcaacccacg tctaccccac aattcactat gaaccacatt cgctccagat gaacttataa
     2521 cctgcaaaat cgctgatgtt tctgcttcaa gaccattttg atcaagttca gggtcaagaa
     2581 gtgtacacgc ttcatatttt ctaggcatct ttcctccgca gtcatttttc ctatttcata
     2641 ctctctattg attatactca tatttcatca attgtcaaaa agcacagacc tttgacataa
     2701 atcgcaaaaa caggtacatt aatatattaa actacccaga tcttttaggg aggcaactat
     2761 gatacgggtc aggtttgcac caagtcccac agggtttctt catataggaa acgcaaggac
     2821 agcgctgttt aactacttgt tcgcaagaaa gaataatgga aggttcattt taagaataga
     2881 agacaccgat acagaaagat caaaacaaga atttgaagaa agcattatag aagatttgaa
     2941 atggcttggt ctaacatggg acgaaggccc tgattgtggt ggatcctttg gtccgtacag
     3001 acaaactgaa cggttacatc tgtacaggga gtatgccaat aaacttctgt cagagggtaa
     3061 ggcttacaga tgctggtgta caaaggaaga aatagaaaac agggtgatgc cgggttcacc
     3121 cgacagggct gcgggatacg ataattactg cagaaaccta acagaatcac aaattaagga
     3181 atttcagacg cagggacgaa aaccagtatt gagattcaag gttccacaca aagaaattgt
     3241 tgtcaacgat atcatcagag gtattgtaaa atttgaacca ggttcactca atgattttat
     3301 aattatgaaa tcaaacgaca tcccaacatt tcattttgcg gtggtcattg acgacctgca
     3361 catgcagatt acacatgtag tcaggggtga agaccatctt tcaaacactc ctaagcatat
     3421 actgattttt gaagccctgg gtcacaccat tcccatgttt gctcacatgt caatgacact
     3481 tggacctgat agaacccgac tcagtaaaag acatggagcc acaagcgtga ggcaatacag
     3541 agaaaaagga ttcctctcgg atgcgatgtt taattatctt gctcttttag ggtggagcag
     3601 caaaaataac caggaaattt tttcacggca ggaattgata caggaatttt cacttgaagc
     3661 atgcaatctc tcaaatgcaa tttttgatcc ggcaaaactt ctatggatta acagtcaata
     3721 tttgcgcatg aaaccagcga aagaaattct tgatctttcc ataccgtttc ttgaaaaagc
     3781 aggttatcat gaatccgact tacagcagca cagagatatt attgaaaaag cgcttgagat
     3841 ggaaaaagag aaattaaaaa ctcttgaaga tgttgcggaa agaacagggt atttcatcac
     3901 agaagatatt tcatgggatg ttgaattgac gaaaaaattc aaaacagacc agacatcgag
     3961 gatactcaat gctttgaaat ctaatctgga acgtcttgaa ccatttgaaa aagaaaacat
     4021 tgaagacatg ataagaaaat tctgtcaaga aaacaatttg aaaacaggcg aggtttttca
     4081 tcctctgaga tttgcgctta caggaacaac aaagggacca ggattgtttg aattgataga
     4141 actgctcggg aaacaacgag ctgtgaagcg aattggtgga tttctaacat atataacaag
     4201 atgatttcga aagcaaaaac acttgaatta aaaaaagcgg caacggagat attgaaaaag
     4261 gcctgcatcg ctgtaaggga cgaggaaatt gcatcaatgg aaatcgttga actcgggctt
     4321 ggaaaaccag aaattattgg attacaaatt atcgtttacg tcaataacga caggtattgc
     4381 gcaaaagaat tgattcttct tccggggcaa acctgcccgg agcataaaca tggccctctg
     4441 ggaccaaaca acccaggaaa gatggaaaca ttcagatgca gatggggaga ggtttattta
     4501 atggttcccg gtcagcaaac tacaccgatg aaagaaaaag tacctgattt cagaaaggaa
     4561 tatttcacag tatttcacga aattgtactg aaacccggag accagtatac actgctccct
     4621 gacacccttc actggttcca ggcagggaac cagggagcaa tcatttcaga attctcttca
     4681 ccaagttttg acgaaaagga tattttcacg gaccccttga taaagagaaa accatgaccc
     4741 gaattttgtt ttttaatacg ctttcaagac agaaggaaga gtttaaacca atagaagacg
     4801 gtgtgataaa aatgtatacc tgtggcccaa cagtgtatga ttatgcacac ataggaaact
     4861 tcagagcata tgtttttgaa gatattcttc atagatttct ggaatatgca ggatacagag
     4921 ttatcagggt catgaacatc actgacattg acgataaaac cattgctggt gcaataaaag
     4981 aaggtgtatc tcttgatcaa tatacagaac ggtatatcaa agcatttttt gaggatatgg
     5041 aatgcctcaa attgaaaaaa gcaacatact ttccaagagc aacccagcac atccctgaaa
     5101 tggtctcttt gataaaacaa ctcattgaaa agggacatgc gtatgaaaaa aatggctcaa
     5161 tatactataa aatcagtaca tttccgaact atggaaaact ttctcattat gattttgaaa
     5221 accttctcac ttcaagtact gatgcggacg aatatgagaa agacgaggtg agagattttg
     5281 ctttgtggaa agccacaaag aacgaaccat ttttctggga cacagaaatt ggcaggggta
     5341 gacctggctg gcatattgaa tgttctgcga tgagcatgaa atatcttggt gaaacatttg
     5401 atattcacac cggtggtgtt gataatatct ttccacatca tgagaatgaa attgcccaga
     5461 gcgaagcagc gacaggcaaa aagtttgtaa actactggtt acactgtgca catcttcttg
     5521 tcaacggcga aaaaatgtca aaatcaaagg gaaattttta tacgctgaga gatctgttga
     5581 acaaaggtta ccacccgtct gaaatcagat atcttcttat gaccacccat tacagggacc
     5641 cattgaattt tacagagcaa tcgcttaaac aggcggaaaa aacaataaag catgtacagg
     5701 cattctatca acgaatttct tttgctctgg aaaatgaaga acattcgcgc caggttcatc
     5761 aggacataga aaaatacatc aatgaagcat cagagggttt tgtcggtgcg ctaagcgatg
     5821 atttaaatat atccctggca atgtcagaag tattcaaatt tatgcacggt atcaacgaaa
     5881 tttgtgaaaa aaacaaaatt acaggcggtg atgcaaaaaa gattattgag ttttttgata
     5941 gaataaatga agtgattgcc attattgaac cggttgaaaa attgcctgaa gaaattgtga
     6001 accttatcaa aaaaagggaa caggcaagaa aagaaaaaaa tttcacactt gccgaccaga
     6061 taagaaacac acttgctgaa tcgggtattg ttcttgaaga caccccgtat ggaacacgat
     6121 ggatcaggaa ataaaaaatc aaaacatgat acctgaagaa agcgcacatt atattatcgt
     6181 tcagggagcg agggaacaca acctcaagaa tatttctgtt aagataccga ggaacaagtt
     6241 tatcgtgatt accggcattt ccgggtcagg aaagtcatcc cttgcttttg acactctcta
     6301 tgctgaagga cagcgaagat atattgaaag tctgtctgct tatgcaaggc aatttctcga
     6361 acagatgaaa aaacctgatg ttgaccatat ttttggtctc cctcctgcaa ttgcaattga
     6421 acaaagaaaa tcggcttcaa atcctcgctc aactgttgcc acgactactg aaatttacga
     6481 ctatctcaga ctgctttacg caagaattgg ccaaccacac tgtccggaat gtcgcagacc
     6541 aatttccagc atatcatcaa gcgagattat tgataaaatt atgacgcttc ctgaaaaaac
     6601 aaaacttctg atgcttgcac cgatcattcg cggaagaaaa ggagaataca gaaaatttct
     6661 tgaagaaatc agaaaatcag gatttttgaa agtccgtata gatggaaaaa tttttgacct
     6721 tgacgacgaa atttctttaa aaagatataa aacccattcc attgaagtcg taattgatga
     6781 acttgagaag tcctcagaca gacagagaat tgctgaaagt gttgaaatgg gattgaaaat
     6841 cggcaaaggt ttacttattg tcaatcagaa cggttcagac cagttgttta atgagaaatt
     6901 tggatgtcct gtctgtggaa tcagttttga ccagcttgaa ccaagaaatt tttcatttaa
     6961 tagcccgtat ggcgcatgtt ctgagtgcaa gggattggga tttttgatga aaatagaccc
     7021 tgaacttgtg atcccagaca gaaataaaac attcagagaa ggagccatca aggcatggca
     7081 ggatactgga ggcaggcaca tatttttcta ttatagggga cttttaagaa gtatgttgcg
     7141 tcacataggg aaaactctgg atacccgtgt atgcgatttg tctgacaggg aaataaattt
     7201 cattctttat ggggatgaag aagagggcta tgaaggggtg attccaaacc ttgaacgact
     7261 ttttcaacaa acagaaagtg aattcagacg tgaagaaatc atgaagtaca tgagggaact
     7321 tgtatgtcct tcctgtaatg gagatagatt aaaaccagaa gtactggcag taacagttga
     7381 aaacaagtcc atcatggatg tgtgtagaat gagtgtttcc tctgtaaagc gattttttga
     7441 gaatcttaat cttatggaaa aacagcaaat gatcgccaga caaattctca aagaaatcat
     7501 aagcagaacg agttttcttg aagaagtggg acttgattat ctgacacttg ataggatgac
     7561 ccataccctt tcaggcggag aagcagaaag aatcagactt gcaacccaga taggttcagg
     7621 attggttggt gtcctgtata ttctggatga accaagcata ggattgcatc caagggacaa
     7681 cagtagattg ataacaacac taaaacatct atcttctctg ggcaatacag tcattgtaat
     7741 agaacacgac gaagaaatga tgagaaatgc tgattatatc atagaccttg gtcctggagc
     7801 agggaaaaat ggaggagaaa ttgttggttg tggcaccata gaagaaatta tgctttctga
     7861 aaggtccatt actggtaaat atctggcagg tatgttatcc attcctgttc caaaaacaag
     7921 acgtaaacca caggacaaat acatttccat tatcggtgca tctcacaaca acctgaaaaa
     7981 catcaatgtt gatatccccc tgggactttt tgtttgtgtc acaggcgtga gtggttctgg
     8041 aaaaagcagt ttgattgacg atgttctcac ccagggcttg cgaaaaatac tttatgatgc
     8101 aaaaacgttg ccaggaaaac acaaagccat tgtgggcata gaaaataccg acaaggttgt
     8161 tatcattgac caatctccaa tcggaagaac cccgaggtca aatccagcca cttacactgg
     8221 catctttgac cttataagaa aattgttcag ttatacacag gaatcaagaa tcaggggcta
     8281 taaacctggc agattcagtt ttaatctgag ggatggaaga tgtcaggcat gtcagggaga
     8341 aggcatcatg aaaattgaaa tgcatttcct tccagatgtt tttattccct gcgatgtttg
     8401 tggtggaaaa agatacaata gagaaaccct tgaaattacg tatcgcggca aaaatattgc
     8461 agaaattctg gcaatgagaa ttgaggaagc gcttgacttt ttcaaacaca ttcatcctat
     8521 atcaacaaaa ctgcagacac tgtgtgacgt cggattgggc tatcttgaac tgggacaacc
     8581 agccacaacc ctttcgggcg gagaagccca gagggtgaaa cttgccgcag aactttccag
     8641 aaagggaaac gaaaaaacac tctacatact tgacgaaccc acaacaggcc ttcatcttta
     8701 tgacatccaa aaattgctgg tagtgttgaa cagattggtg gacaaaggaa gcactgttgt
     8761 ggttattgaa cacaatatag aagtgataaa gtctgccgac tatatcatag acctcggtcc
     8821 tgaaggcgga gacagaggtg gtcaggttgt ttgtactggg actcctgagg aagtcgcaaa
     8881 aaataaaaaa tcgtacacag gacagttttt aaacagggta ctttatcaga aaattacaac
     8941 cgttgcgtga ggaggtttat atggtcgcaa atcttcctcc acattatcat acaaaagaag
     9001 cagaattaaa aaacgcaaaa acaccagaag aaaaaattgc cattctcctt gagatgatgg
     9061 caatcatgcc aaaacataaa gggactgaaa aacttcaaaa agaactcaaa tcaaaaattg
     9121 caaaactcag gaaggaagca tctgaaaaaa aggtaatatc aaggggaagt cctgttccaa
     9181 caattgaaag ggaaggtgca ggccaggtta tcattgcagg tccgccaaat actggaaaat
     9241 caagccttct tgcagcaata acaaaagcaa agccagaaat tgcagattat ccattcacaa
     9301 caaaaattcc tcaaccagga atgcttcaat atcaggatat acagattcag gtaattgaca
     9361 gtcctgccct ttcgtcagga atttctgaaa actggctcgg cgatatgatg cgaaaaagtg
     9421 atgtaattct tctccttctt gatctctctg acgatgatat acttgaaaaa atggatgaaa
     9481 ccatgcaggt attggaacaa ttcaacataa aaacacccgg tacctgggaa ttcacaaaaa
     9541 agatactatg ggctggaaat aaaatagata tcccttcgtg gaaagacata aaaaatgtgc
     9601 tatccgaact ttacccgata ggcgatcaca ttataccagt ttctgtatta caaaaaatta
     9661 atcttgactt gcttgcagaa caaatattta taaaacttga tattatcagg gtttatacaa
     9721 aaattccagg caaaccacct gaaatgaaag acccttatac cattgcggcg aattccacac
     9781 ttgaggacct ggcaaaatcc attcacaagg acctggttaa aactttcaaa tacgcaagat
     9841 tgtggaagca gggaacagga aatccagtca ttgcaggcag ggactatgtt cttgaagaca
     9901 gagacctggt ggaaatacat gcagaggaaa tgaaatgaag gtaaaacttg ctgctgagaa
     9961 tatcataaaa aaatataatg agacaaccat ccttgacagc ataaccatca gggttgatag
    10021 atctgaattt tttgccctgc ttggaccttc aggaagtggt aaaacaacac tacttcgaat
    10081 cattgcagga tttgtcgtcc cggatggtgg aaagatcctt attgaagaaa aagatattac
    10141 atatattcca gccagcaaaa gaaacatggc aatggttttt caaaattata gtttgtggcc
    10201 acatatgaca gtttttgaaa atgtcgcctt cgggctcaga ataagaaaaa tacctgtcaa
    10261 tgatatccag gaaaaggtca acagagtgct tgacatggtt gatttacaac aacattccac
    10321 aaaaaaacca cagcaacttt ctggaggaca acaacaacgc gtggctcttg caagagcact
    10381 ggttgttgaa cctgacattc ttcttcttga tgaaccgtta agcaaccttg acgcacacct
    10441 cagagaccaa ttaagacagg aaattaaaac actacagaaa aatcttggaa tcaccacaat
    10501 atacgtgaca catgaccaga aggaagcaat gtatcttgcc gacacaattg cagtaatgct
    10561 tgatggtaaa attgttgaaa cgggacggcc agaaactatc tatcaaaaac cctcgtcttt
    10621 acagaccgcg agatttcttg gacagttgaa tgaaattcgt ggcgttgtga aaaaaatcaa
    10681 aaatggttca tattacattg aaacagacat tggaatgctt gaatcaaaat cggaacatga
    10741 attttctgtc aatcaacagg tgatcgcagg attcaggcca gaatttatcc aattcccggc
    10801 acaccaggat acagccacca aaattgaatg caaacttcat tcacatgaat acgcaggcac
    10861 tttcatcaat gcattgttaa agaaaaacaa catacttttc aaagcgacac tatcccctgt
    10921 atcattcaat caacagaagg acaggattta tgtaatcggg attgacccgg aaaacatttt
    10981 gctgttcaaa atagaacaat gaaaaattta ttttttgcac tattgttgtt tttcatcatc
    11041 tttacaggtt atccacttgt atttgttttc tggaaatcag ctgatgtttc cattcacctt
    11101 tttcctgttg tatggtcaaa catcagtgta cgccagagtt ttattaatag catttttctc
    11161 gcaatttcag tgaccattgc aaccagtatt attggttttc cactggctta tgtaatgagt
    11221 tttgtaaaaa tacccatgaa aaatttctgg cgaaaagcat ttcttcttcc tcttgtggca
    11281 ccacctttcg tttctgccat agccatgaga cagattctct cacgttttgg ccccgtcaat
    11341 ctggtactta tgaaactcgg cattacaaca acacccgtag attttcttgg aaccggactt
    11401 acaggaattt tcatactcca ggtccttcat ctatatccca ttatttattt gaacattctt
    11461 tcttcccttg aaaatataga aggttcctgc attgaagccg cggaaaatct tggtgcttct
    11521 gtatttcaaa atttcagaaa aatcattttt ccactttctt taccaggata tttcgccggc
    11581 gcattccttg tgctcatatg gtccctcact gaccttggta cccccattgt atttgattat
    11641 ccgaacctga ttccggtttt aatatttcaa tcccttacag acatttattc aaatccagcc
    11701 ggttatgttc tcatcatatt tatctgtatt cttgcaatga tactgttttt ctttaccagg
    11761 tatttgctgg gaaaaatgtc tttcgtgggc atagcaagat ataagacaac aatggacccc
    11821 ctgcacgttc ctgcaagtgt atcaatagca atttcggttt ttctcgggtt tttagttttt
    11881 gtctcactcc ttccacacat atcagtcatt cttaattcaa tttcccagaa atggtttttc
    11941 acaattcttc cagatagatg gacaattcaa tattatagag atgtattcct tcatccaatt
    12001 gcaagaatca gtctgattaa cagcacggtt tattgtatta ttacagggct attgattatg
    12061 atcatcagtg ttgcaacagg cctcattctg tccagatcgc aaataaaggg gaaaaatatt
    12121 cttgaaagtt ttgtcattct tccaatggct gtgcccggca ttgtcttcgc gttctcctat
    12181 gtccttgcgt tctcagaaac catttttgac ccgagaaatt ttccaatgtt tctgcttata
    12241 gtggcttatg ttctcagacg tttacccttt gcagtaagag cggttgtcgc aaactttcaa
    12301 cagattgata tttcctgtga agaagccgca tttaaccttg gtgcatcaag atggaaaacc
    12361 tttgtcagta ttacaatgcc aatgttaaga aacggaatta tagcagggtt catattctct
    12421 tttgcgtttt ctatgatgga ggtttcaagt agtttaatac tggtgaacaa acaacagtat
    12481 tttccgattg caaaaggaat ttaccagctt gccggaagaa taactgatgg cccttatatt
    12541 gcatctgctt taggagtact tggaatgatt ataagctttg cggctttaat gatcatccag
    12601 agaatatctg gaaaacggga tatatccttt tctatttaga atgtgataaa ataaaaagca
    12661 tgggcggttg gctcaggggc aagagcgcct ccctcacacg gaggaggccg caggttcaaa
    12721 tcctgcaccg cccacatcgt tttcttacag aagcaggatt tgaatggagc ggtgctggtg
    12781 cgaggaagaa cgagcaccgt atagcactaa gggcgagaag gtgagcccgt tgaccgaagc
    12841 gcagtgtgca ag
//
LOCUS       DAORYU010000012        12590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_3919_length_12590_cov_4.970323, whole genome shotgun sequence.
ACCESSION   DAORYU010000012 DAORYU010000000
VERSION     DAORYU010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12590
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3919_length_12590_cov_4.970323"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /gene="rpiB"
                     /locus_tag="PLW07_00875"
     CDS             complement(<1..419)
                     /gene="rpiB"
                     /locus_tag="PLW07_00875"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOL49109.1"
                     /translation="MRIGIASDHAGYEMKKYLIAYLKKEGYEIMDFGPDVLKKVDYPD
                     YGARVASELSAGKIDRAILVCGTGIGMSIVANRFPRVRAALCYDPFLAEIARIHNDAN
                     ALALGGRIIANELACEITRVFLETKFGGGRHLRRIKK"
     gene            complement(416..1405)
                     /locus_tag="PLW07_00880"
     CDS             complement(416..1405)
                     /locus_tag="PLW07_00880"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOL49110.1"
                     /translation="MAHIVNLMENSQEVVLEKVMMALKNSNVAIVPTDTVYGIVCDGK
                     NDFAKENLYILKNRPESKPLIGFTKDMEMAKKVAHISENFLPFITKRWPGRNTFIFQS
                     KIESNHIVGKDKTIALRIPDYSFINNLCDSFPMLASTSANVSFSKSACCIDEIDSELL
                     EKVAIVVDGGRISGRESAIWDMTENIPRLVRGRVLFVCSGNSCRSPMAQALLKNIVAD
                     KKIEVASAGTDADFSGRASQSAIDVLKETGINFEDFVTTSLNERIIEDADLIFVMEEK
                     HREKVLQRKPEALDKIFILNVPDPAGGNIFQYREIRDIIRERIKKEVLTRMKI"
     gene            complement(1389..2210)
                     /locus_tag="PLW07_00885"
     CDS             complement(1389..2210)
                     /locus_tag="PLW07_00885"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOL49111.1"
                     /translation="MKIGRFRRNQDVFTGIVEGNKIGSIGDIPVEHIIANNLTGSVRA
                     LISSWFSLEELEYAPLIKPCKIICLGLNYKSHAEEGGWKLPSEPVYFEKSSSCIIASK
                     EAIRIPDNIGRIDPEVELAVIIGKTCKNVSKQEAISCIAGYTIANDVTARAMQKQDQT
                     NKYPWFRSKSMDTFCPVGPWFVTSDEIDWQEPLKIELRINGETKQSSSTDRMIFQIPD
                     IICSITRYLTLYPGDIISTGTPEGISPIKNGDIVECEIEKIGLLSNPVVSYGTHS"
     gene            complement(2233..4239)
                     /gene="uvrB"
                     /locus_tag="PLW07_00890"
     CDS             complement(2233..4239)
                     /gene="uvrB"
                     /locus_tag="PLW07_00890"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOL49112.1"
                     /translation="MEKIFKLKSQFTPAGDQPAAIQKLARNLNSGAKWQVLLGVTGSG
                     KTFTMANVIERVNRPVLVISHNKTLAAQLYSEFRQFFPENKVRYFVSYYDYYQPEAYI
                     PATDTYIEKDASINEDIDRLRLAATSAILSSRDVIIVASVSSIYPLGSPEDHRALMLN
                     LTLNQRTERTAILRRLVEIQYERNDFDFQRGRFRIRGNTIEIIPSYEETGVRIVLSGE
                     EIACLERFDPLTGATISKEEQISIYPAKHFVSPSDKIERALRTIREELQQHLEKFKNQ
                     GKLLEAERLEMRTNNDLEMLAELGYCHGIENYSRHLAGRLPGERPECLLDYFPSDYLM
                     FIDESHMTIPQIRGMHAGDRSRKETLVEYGFRLPSALDNRPLTFDEFESMINQVICVS
                     ATPGPYELEKIGVRKLGEKSELLVEQVIRPTGLIDPPIKVLPTKNQLDDLVVRIRERI
                     NKKQRVLVLTISKQLAEQLSDYLKDLHLKVMYLHYEIDTLQRMEILKKLREATFDVLV
                     GINLLREGLDLPEVSLVAILDADKEGFLRSERSLIQIAGRASRSIDGEVIMYADKITD
                     SMKAAIDETERRRAIQQEYNRIHNITPKQVIKPIYQTISDIIGSKSRKQQEIIADKEE
                     ILYGKNLAQLIRGLEKKMISYAKQLDFETAIKYREKIKRLKNEK"
     gene            complement(4245..5357)
                     /gene="dnaJ"
                     /locus_tag="PLW07_00895"
     CDS             complement(4245..5357)
                     /gene="dnaJ"
                     /locus_tag="PLW07_00895"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOL49113.1"
                     /translation="MAEKRDYYEVLGVSKNATIDEIKKAYRELALKYHPDRNPGNKEA
                     EEKFKELTEAYEVLSDPEKRKTYDQFGHAGFGPQGFDWRQDFGRVRMDFSDLFGDFFS
                     DFFSDIFGREERQPSNRGEDVEMRISIPLRDAVKGTERTINVSVYEHCPTCNGTGSKS
                     KKSDLECLQCHGTGKVMYQQGFITISQVCPRCHGQGTVIPDPCMNCRGTGRVRVMHKI
                     NVKIPAGIEHGTSLRLKGMGNAGTRGGSRGDLYVTVFVEQDDFFTRKGNDLFCEVPIT
                     VTTAVLGGEIEIPTLTGKVKAKVSPGTQDGTILRLKNLGVPRGSTHGHLFVVLRVMMP
                     RSLSREEKRLWEQLKSIEDERNYPEVTKYKQKLFGS"
     gene            complement(5360..6559)
                     /gene="argJ"
                     /locus_tag="PLW07_00900"
     CDS             complement(5360..6559)
                     /gene="argJ"
                     /locus_tag="PLW07_00900"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:TIGR00120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HOL49114.1"
                     /translation="MKTRTGVCFPDGFRASGINCGIKKERKDLAVIISEQDCIAAACS
                     TTNRVKSYSLLWALKNIKNPVRAILINSGNANVLGGDTGWDITCNIMRKFADAMRVDI
                     SQVLFASTGVIGKLLPEDKITRSIEQLVNQASENGGMDAAEAIMTTDRFPKHIEIETQ
                     IQARSKNTRVRIGGMAKGAGMICPNMATMLGFITTDAVISQSALKKALKDAVDKSFNM
                     ITVDNDQSTNDFVVCMANGMAGNRTIHQDTEDYGKFATALTEVCVFLARKIAENGEGS
                     DRLIEVHVCGAWSQKDARRIAKKIAGSNLVKAAVTGTWPNWGRIAAAAGSACSRFDPL
                     KMKISIGKYDVFDGAPCDIDETIVRKELSKKQILITVSLNAGKESATAWGCNLTEEYV
                     RINMEKE"
     gene            complement(6580..7251)
                     /locus_tag="PLW07_00905"
     CDS             complement(6580..7251)
                     /locus_tag="PLW07_00905"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IspD/TarI family cytidylyltransferase"
                     /protein_id="HOL49115.1"
                     /translation="MIVAAGRGVRFKSNLPKILFPLDGIPVIEYSLRTFSMCSLIDEI
                     VLVVNKQMMESDYVSKWNGEFSKIREIVAGGACREESVFNGISRCSKKCDIILIHDGA
                     RPFVTESLISSVIKGTKKYGACIPAYSVNGSVKLVKRKRLVSTIVEKGIEVAQTPQGF
                     KREILEKIFLTCGNQLNKYPDESSMCEQSGISVYVVSGEITNIKITTQDDVKIALSLL
                     NTDSV"
     gene            complement(7260..8231)
                     /locus_tag="PLW07_00910"
     CDS             complement(7260..8231)
                     /locus_tag="PLW07_00910"
                     /inference="COORDINATES: protein motif:HMM:NF014047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HOL49116.1"
                     /translation="MGLYFIRATFVLISAFAGSVFWMENKLNGVIAGLVGSLIVVGIE
                     LLMQKIPFRKFIMGVIGLIVGLVTAILIANFVLLVPFATPRQEDGVRFVLYFIFSYLG
                     IMIGIRGVGELGFLFPYLHQFKAEAMRAVVIDSSVAIDGRVYELAISGFIEGTIVIPT
                     FIIRELQDLADSASEMKRQRGRRGLDTLNKMRNDSNLDVKIYDVDYPDVEGVDAKLVK
                     LASSIGARVLTNDFNLTKVAEVKNVKVLNLHNLATLMRAKLMAGETLQLKVIREGKEH
                     GQGVGYLEDGTMVVIENGAKYIGKTVETVIDSTIQTVSGRIIFAKVK"
     gene            complement(8288..9643)
                     /gene="radA"
                     /locus_tag="PLW07_00915"
     CDS             complement(8288..9643)
                     /gene="radA"
                     /locus_tag="PLW07_00915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOL49117.1"
                     /translation="MTKEKKIFSCQECGYATVRWLGKCPSCGAWNSFHQETISHHGPM
                     TEYEKQKPVSLGEIKVGQNIRIPIGTGELDRVLGGGLVGGSVILLAGEPGIGKSTLVL
                     LSASKLSGQGKKVLYVSAEESLDQLAMRADRLGVKNTDSLFFLTENEIKTAGDALETI
                     KPDVVIVDSIQMMYDSEFDYPYGSIFQVRRTAQFFIEWAKRHGSPVFLIGHITKEGNI
                     AGPKILEHLVDVVLYFEGDKLSNLRVLRAIKNRFGSTDEIGVFRMDEHGLIEVPDSAR
                     LFLSTSSTNQPGLVVFPAQEGKRTILLEIQSLITPSYLGVPRRTFTGLDYNRVNLVLA
                     VIEKKLRMNLASRDIYFNVAGGLKIAEPGVDLAIAIASISSYKDIPAVSNTVFIGEIA
                     LTGEIRPVQHIVSRLKEALRLGFRQAFIPLGSEITSFPGMNIVEVSWLGDLPSLAFEK
                     G"
     gene            complement(9645..10796)
                     /locus_tag="PLW07_00920"
     CDS             complement(9645..10796)
                     /locus_tag="PLW07_00920"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOL49118.1"
                     /translation="MKTKSFNLQFRVGLFIFLGLIIFFVFIFSQGKILRGKGYELKIA
                     FNYIGGLDPGAPVRVSGYRVGEVRSIDLVMEQDQPRIIVTVRIKPEVRLGRHSRFMVR
                     NYGIIGEKYMEIMSTGLKDVPLIEPGETLKGEDPLPLEKFLSAGEDILKNVNNLLVSL
                     NNVTSDENLKKQLLQIVQGTNQVLLKASFAIDNINVLASNWNKTSLELNKTIAGIRPE
                     ISDLITSAKSASQEINKIVLSNSDRINNIISNLDQSTDKLEQTMLEVSTSIKNVSETF
                     VKTGTKINNFVDKVENQGLVADLISDTEIVNDIKITMKNLKQTSLDLDIALTKLGYAS
                     EQLASILSDIRAGKGTVGKLVAKDDLYNQIFEMIQDLRAHPWKILFRGR"
     gene            complement(10777..11484)
                     /locus_tag="PLW07_00925"
     CDS             complement(10777..11484)
                     /locus_tag="PLW07_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOL49119.1"
                     /translation="MKILKEISLNVAENETVVILGPSGSGKTVLLKTLLGLTTPSDGK
                     LIVLGKDMFNISKEELLQLRKKTGVVFQSAALFDSMSIWENVGFHLLEFLRLPEEEVR
                     KKAEQMLLAVGLSGILDKMPDELSGGMRKRVGIARALMSEPEVLFYDEPTAGLDVITS
                     ASITKLMKKIHMEFQTTDLIVTHDLSIARYLADRIAVIKEGKIIETGTWEQLLHSKDE
                     FVKYFLNIGAAYENEKF"
     gene            complement(11528..12307)
                     /locus_tag="PLW07_00930"
     CDS             complement(11528..12307)
                     /locus_tag="PLW07_00930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOL49120.1"
                     /translation="MIGLINSLGKLTLEAICYLGEITFITTSAIKKIFTDAFRRKKLL
                     FEQMAKIGIDSFPLVGIIASFTGMVMVMEIALTLKKFGAESYVGGIVSVSMVRELGPI
                     IVALIVAGRVGASVAAEIGSMKITEQIDALEVFGVDPVSYLVTPRLFASMVMMPILTI
                     MANFLSIIGGYIIAVYLVHITSGVYLYQSFEYITLKDILVGLAKAFVFGILITVASCL
                     EGLKTSGGAEGVGISTTRAVVHAFFLVILANLVLTAIFYFL"
     gene            complement(12304..>12590)
                     /locus_tag="PLW07_00935"
     CDS             complement(12304..>12590)
                     /locus_tag="PLW07_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012771429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HOL49121.1"
                     /translation="SIPVIALSQMNRLIERREDKRPILADLRESGSIEQDADLVIFIH
                     REEQYDESEETKGLTDIIIAKHRNGPIDRVHLTFLKNCLRFENCSKRQPT"
BASE COUNT         3513 a         2882 c         2139 g         4056 t
ORIGIN      
        1 atttttttta tccttctcaa gtgtcttcca ccaccaaact ttgtttcaag aaaaaccctg
       61 gtaatctcgc aggcaagttc attggcaata attcttcctc cgagcgcaag cgcgtttgcg
      121 tcgttatgaa tacgggcaat ttctgcaaga aaagggtcat agcataatgc ggcacgaacc
      181 cggggaaatc tgtttgccac tatactcata cctataccgg ttccacaaac aagaattgcc
      241 ctgtcaattt ttcccgcaga caattctgat gctacccgtg ctccataatc aggataatca
      301 acctttttca gtacatctgg tccaaaatcc attatttcgt atccttcttt cttcaaatac
      361 gcaatgagat attttttcat ttcataaccc gcatggtcgc ttgcaattcc tattctcata
      421 ttttcatcct tgttaggact tcttttttta ttctttctct gattatatcc cgaatttcac
      481 gatactgaaa aatattacct cctgcagggt ctggaacatt aagaatgaaa attttgtcca
      541 gggcttctgg ctttctttgc aatacctttt ccctgtgttt ttcttccata acaaaaatca
      601 ggtctgcatc ttcaataatg cgttcattca atgatgttgt cacaaaatct tcaaaattaa
      661 ttcctgtctc tttcaaaacg tctattgctg actgagatgc ccttccagaa aaatctgcat
      721 ctgtgcctgc ggatgccact tctatctttt tatccgcgac aatatttttc agcaatgcct
      781 gtgccatagg acttctgcat gaattcccag aacatacaaa aagtactctc ccgcgaacaa
      841 gtcgcggaat gttttctgtc atatcccaaa tggctgattc cctacccgaa attcttccac
      901 catcaactac aatggcaacc ttttcaagaa gttctgaatc tatctcgtca atacagcagg
      961 cacttttaga aaacgaaaca ttggcgcttg ttgaagcaag cattgggaaa ctgtcgcaca
     1021 gattgttgat gaaggagtaa tcaggaattc tcaatgcaat ggttttatct tttcccacaa
     1081 tgtgatttga ttcaattttt gattgaaaaa taaatgtatt tcttcctggc cagcgttttg
     1141 taataaaagg aaggaaattt tcagagatat gggcaacttt ttttgccatt tccatatctt
     1201 tggtgaaacc gataaggggt tttgattctg gtctgttctt gagaatatat aggttttctt
     1261 ttgcaaaatc attttttcca tcacatacga tgccatacac ggtatcagtg ggcactattg
     1321 caacatttga atttttcagc gccatcataa ccttctcaag aacaacttcc tgagaatttt
     1381 ccataagatt aactatgtgt gccatatgaa accacaggat ttgaaagaag acctatcttt
     1441 tcaatctcgc attccacaat atcaccattt ttaatgggac tgattccttc tggagttcct
     1501 gttgaaataa tgtcaccagg atacagcgta agatatcttg taattgaaca gataatatcc
     1561 ggtatttgaa aaatcatcct gtctgtactg gaactctgct ttgtttcacc attaattctt
     1621 aattcaattt tcaatggttc ctgccaatca atttcatccg atgtaacaaa ccatggaccc
     1681 acaggacaaa atgtgtccat gctttttgat ctgaaccacg gatatttatt ggtctgatcc
     1741 tgtttttgca ttgcgcgggc tgtgacatca ttagcaatgg tataaccagc aatacatgat
     1801 atcgcctctt gtttggaaac atttttacag gtttttccaa taatcacagc aagttccact
     1861 tcagggtcaa tccttcctat gttatctgga atgcgaattg cttccttgct ggcaataatg
     1921 caggatgagg acttttcaaa atacactggt tctgagggca atttccagcc accttcttca
     1981 gcatgacttt tataatttaa tccaagacat atgattttac acggcttaat aagtggcgca
     2041 tattcaagtt cttccaggga aaaccatgat gaaataaggg ctctgacact gccggtaaga
     2101 ttattagcaa taatgtgttc aacaggaatg tcaccaatgg aaccgatttt atttccttca
     2161 acaattccag taaaaacatc ctgatttctt ctgaatcttc ctattttcat ttttgcctcg
     2221 ctttttcaat tttcattttt catttttcaa tcttttgatt ttctctctgt atttaatggc
     2281 tgtttcaaaa tcaagctgct tcgcatatga tatcatcttt ttttcaagac ccctgatgag
     2341 ttgagccagg tttttcccgt ataaaatttc ttctttatct gcaattattt cctgctgttt
     2401 cctgcttttg cttccgataa tatcacttat tgtctgatat atcggcttga taacctgttt
     2461 tggcgtgata ttgtgaatac ggttatattc ttgctgaatc gctcttcttc tttctgtttc
     2521 atcaattgct gccttcattg aatctgtaat tttatctgca tacataatta cttctccgtc
     2581 aatacttctg gacgccctgc ctgctatttg aatgagggaa cgttctgatc ttaaaaatcc
     2641 ttctttatca gcatcaagaa ttgcaacgag actcacttca ggaaggtcaa gcccttcgcg
     2701 caaaagattg attccgacaa gaacatcaaa tgttgcttct ctcagttttt tgagaatttc
     2761 catgcgctgg agggtatcaa tttcataatg gagatacatc actttcaggt gtaaatcttt
     2821 caaataatcg cttaactgtt ctgcaagttg tttactgatt gtcagaacaa gcaccctctg
     2881 ttttttgtta attctttcgc gaattctcac aacaagatca tcaagttgat tctttgtggg
     2941 aagcacttta atgggtgggt caataagccc tgttggtctg atgacctgtt caacaagcag
     3001 ttcagacttt tcaccaagtt ttctcactcc aattttttca agttcatacg gtccaggggt
     3061 tgcagaaaca cagatgacct ggttaatcat tgattcaaat tcgtcaaatg tcaggggtct
     3121 attgtcaagt gctgaaggaa gtctgaaacc atactcaaca agcgtttcct tgcgggacct
     3181 gtcgcctgcg tgcattccac gaatttgagg aatagtcata tgcgactcat caatgaacat
     3241 taaataatcc gatggaaaat agtcaagcaa acattccggc ctttctcctg gcaatcttcc
     3301 ggcaagatgc cttgaataat tctcaatacc atgacagtat ccaagttctg ccagcatttc
     3361 aaggtcgttg tttgttcgca tttcaagcct ttctgcctca agaagttttc cctggttttt
     3421 gaatttttca agatgttgtt gaagttcttc cctgattgtt cttaaagccc tttcaatctt
     3481 gtcagaaggg ctgacaaagt gttttgcagg ataaattgaa atttgttctt cttttgatat
     3541 tgttgcgccg gtcaaagggt caaatctctc aagacaggca atttcttctc cagaaagaac
     3601 aattcttacg cccgtttctt cgtaagaagg aatgatttct atagtgttac cacgaatcct
     3661 gaatctacct ctctgaaagt caaaatcgtt tctttcatat tgaatttcca ccaatcttct
     3721 caaaattgct gttctttctg tccgttgatt caatgtgaga tttaacatta aggcacgatg
     3781 gtcttcggga ctgcccagcg gatatattga ggaaacactt gcgacaataa taacatccct
     3841 gctggacaaa attgcgcttg tcgcagcaag ccgtaatcta tcaatgtcct cattgattga
     3901 agcatccttt tcaatatagg tatcagtggc aggaatgtac gcttctggct gatagtaatc
     3961 gtaataactg acaaaatacc gaaccttatt ttcagggaaa aattgtctga attcgctgta
     4021 cagctgggca gcaagtgttt tgttatgaga tatgacaaga acaggtctgt ttaccctttc
     4081 aatgacattt gccattgtga atgtttttcc actgcctgtg accccaagta acacctgcca
     4141 tttcgcaccg ctgttaagat tccttgccag tttctggatt gccgcgggtt gatcgccagc
     4201 aggtgtgaac tgtgatttaa gtttgaagat tttttccatt catttcaaga accaaataat
     4261 ttctgtttgt actttgttac ttcaggataa tttctttcat cttcaatcga tttcaattgc
     4321 tcccagagac gtttttcttc gcgtgatagt gatctgggca tcatcacccg aagtacaacg
     4381 aaaagatgcc catgtgtgga accccgtggt acaccgagat tttttaatct cagaattgtt
     4441 ccatcctgag taccaggaga tacttttgct tttactttac cggttaatgt tggaatttct
     4501 atttctccac cgagaactgc tgttgttact gtaatgggaa cttcacagaa aaggtcattt
     4561 ccttttcttg tgaaaaaatc atcctgttca acaaaaacag tgacataaag atctccacga
     4621 ctaccccccc tcgtaccggc atttcccatt cccttcaacc tgagagatgt tccgtgttca
     4681 atgccagcag gaattttcac attgatcttg tgcattactc taacccttcc tgttccacga
     4741 caattcatac acggatctgg gattactgtg ccctggccat gacatcttgg acatacctga
     4801 ctgattgtaa tgaatccctg ctgatacatt accttacctg ttccgtggca ttgtaaacat
     4861 tccagatcag attttttact cttgctacct gttccattac atgttggaca gtgttcgtat
     4921 acagaaacat tgatggttct ctctgttccc ttcactgcgt ctctcagtgg gattgaaatc
     4981 cgcatttcaa catcttcgcc gcgattagaa ggttgtcttt cctctctacc aaaaatatct
     5041 gaaaagaaat cactgaaaaa gtcaccaaaa agatcagaaa aatccattct gacccttcca
     5101 aaatcctgtc tccagtcaaa tccctgggga ccaaaacctg catgaccaaa ttggtcatag
     5161 gtttttcttt tctcagggtc actcaatact tcatacgcct cagtgagttc tttgaatttc
     5221 tcttctgctt ccttgttccc tggatttctg tctggatgat acttgagggc tagttccctg
     5281 tatgcctttt ttatttcatc aatggtcgca ttcttgctga caccaagaac ctcatagtaa
     5341 tctcgttttt ctgccatgtt tattctttct ccatattgat tctgacatat tcctcagtca
     5401 gattacatcc ccatgcggtc gcactttctt tgcctgcatt aagagatacc gtaataagta
     5461 tctgtttttt tgataattct ttccgaacaa ttgtttcatc aatatcgcac ggcgcgccat
     5521 caaacacatc atatttgcca atagaaattt tcattttgag cgggtcaaac ctgctgcaag
     5581 cagaacctgc tgcagcagcg attcttcccc agttaggcca ggttcctgtc actgcggctt
     5641 tcacaagatt tgagcccgca attttttttg caattcttct ggcatctttc tgagaccatg
     5701 ctccacaaac atgcacctca atcaatctgt cagaaccctc tccgttttct gcaatttttc
     5761 tggcaagaaa tacacatacc tctgtcaacg ctgtggcaaa ttttccgtaa tcttcagtat
     5821 cctgatgtat tgttctgttt ccagccatac cgtttgccat acagacaaca aaatcgtttg
     5881 tactctggtc attatccact gtaatcatat tgaaactctt atcaactgcg tctttcaggg
     5941 cttttttcaa cgcactctgt gaaatgacgg catctgttgt gataaaaccg agcattgtag
     6001 ccatattggg gcatatcatt cctgcgccct ttgccatgcc accaattcga acacgggtat
     6061 ttttacttcg tgcctgaatt tgcgtttcaa tctctatgtg tttcggaaat ctgtctgtgg
     6121 tcataatcgc ttctgccgca tccatgccac cgttttctga cgcttgattc acaagttgtt
     6181 caatactacg agtaattttg tcttcgggaa gcaatttccc tatcacccct gttgaagcaa
     6241 aaagaacctg actgatatca actctcatcg cgtctgcaaa ttttctcatg atgttacagg
     6301 ttatatccca gccagtatct ccaccaagaa catttgcgtt tccactattt ataagaattg
     6361 ctcgcacagg atttttaata tttttcagtg cccacagaag agagtaggac tttaccctgt
     6421 ttgttgtgga gcacgccgca gcaatgcagt cctgttcaga aataataact gccaggtctt
     6481 ttctttcttt cttaatgcca caattgatac ctgatgccct gaaaccatct ggaaaacaaa
     6541 cacctgttct tgttttcatg ttcttccttt tcataaaatt caaacagaat ctgtgttgag
     6601 caaagaaagt gctattttca catcatcctg ggttgtaatt tttatgtttg ttatttcacc
     6661 agaaacaacg taaacactaa tacctgattg ctcgcacatt gaagattcat cgggatactt
     6721 atttaattga ttcccgcatg ttagaaaaat tttttcaagt atttctcttt tgaatccctg
     6781 cggtgtttga gcaacttcta ttcctttttc aacaattgta ctgacaagtc tcttgcgctt
     6841 cacaagtttc accgaaccat ttacagaata tgcgggaata cacgctccgt atttttttgt
     6901 accctttatt acactggata taagagattc tgttacgaat ggccttgcgc cgtcgtgaat
     6961 aagaataata tcacacttct ttgaacaccg tgaaatccca ttaaaaactg attcttccct
     7021 gcacgcacca ccggcaacta tttccctgat tttggaaaat tcaccgttcc attttgaaac
     7081 ataatcactt tccatcatct gtttattcac aaccaggaca atttcgtcta tcaaagaaca
     7141 cattgaaaat gttctgagac tgtattcaat aacaggaatt ccgtccagag ggaataatat
     7201 cttgggtaga ttgcttttga atcgaacccc tctgccagca gcaacaatca cacagcccgt
     7261 catttcacct tggcaaaaat gatacggcca ctcactgttt gaatggtact atctatcacg
     7321 gtttcaactg tcttaccgat atattttgcg ccattttcaa tcacaaccat tgtgccgtct
     7381 tcaagataac ccacgccctg cccatgttcc ttgccttccc tgatcacctt caactgcagt
     7441 gtctcgcctg ccatcagttt agccctcatc agggtcgcaa ggttatgcag attcaatact
     7501 ttgacgtttt ttacctctgc gacttttgtg aggttgaaat catttgtcaa aacccttgcg
     7561 ccgattgaag atgcaagttt cacaagtttt gcatccacac cttctacgtc aggatagtca
     7621 acatcgtaaa tttttacatc aagattactg tcatttcgca ttttattcaa agtatcaagc
     7681 ccccgccttc ctcgctgtct tttcatctca cttgctgaat cagccaggtc ctgaagttcg
     7741 cgaataatga acgtgggaat cacaatggta ccttcaataa aaccacttat ggcaagttca
     7801 tataccctgc catctatcgc cacactacta tcaatgacca cggctctcat cgcctcagcc
     7861 ttaaactggt gaagatacgg aaatagaaat ccaagctcac cgacacccct gataccaatc
     7921 atgatgccaa gataactgaa aataaaatac agaacaaacc tgacaccgtc ttcctgtctt
     7981 ggggtagcaa aaggaacaag tagaacaaaa ttggcaatca aaatagctgt gaccagacca
     8041 actataagcc cgataacccc catgataaat tttctaaatg ggatcttctg catcaaaagt
     8101 tcaattccaa caacaattaa acttcctaca agcccggcta taacaccatt caatttgttt
     8161 tccatccaga acacagaacc agcaaaagcg ctgatcaaca caaaggttgc tctgataaaa
     8221 taaagtccca ttgttcacct cctgaaataa tgttctggga tattatacat caaggttgtt
     8281 aaaatgttca acctttttca aatgcaaggg aaggcagatc tccaagccag cttacctcaa
     8341 caatattcat tcccgggaat gaggtaattt cagaacctaa ggggataaat gcctgcctga
     8401 aaccaagacg aagggcctct ttcaaacggg atacaatatg ttgaacaggt ctgatttctc
     8461 ctgtaagggc aatttcacca ataaaaacag tattggatac agcagggata tccttgtatg
     8521 acgaaattga tgctatggca atggcaaggt ccacgccggg ttcagcaatt tttaatcctc
     8581 cggcaacatt gaaatagatg tcacgcgaag caagattcat gcgaagtttt ttttcaatca
     8641 ccgccagcac aagattcacc ctgttgtaat caagacctgt aaatgttctg cgtggaacac
     8701 cgagataact tggagtaatc aaactttgaa tttcaagcag aattgtgcgt ttgccttcct
     8761 gagcaggaaa aacaacgagt cctggctgat ttgtagaact ggtggaaaga aacaatctgg
     8821 ctgaatcagg aacttcaatc aacccgtgtt catccattct gaaaacaccg atttcgtcgg
     8881 ttgacccaaa tctgttttta atagccctta acaccctgag gtttgacagt ttatctcctt
     8941 caaaatacag aacaacatca acaagatgtt caagaatttt tggtccggca atattaccct
     9001 ctttcgtaat atgtccgatt aaaaaaacag gcgacccatg cctttttgcc cattcgataa
     9061 aaaactgagc ggtgcgtctc acctgaaaaa ttgaaccata cggataatca aattctgaat
     9121 catacatcat ctgaatagaa tcaacaatta caacatcagg tttaattgtt tcaagggcat
     9181 ctcctgctgt ttttatttca ttttcagtaa gaaaaaaaag ggaatctgta tttttgacac
     9241 cgagcctatc ggcgcgcatt gccagttggt caagagattc ttcagccgaa acataaagta
     9301 ctttctttcc ctgtcctgaa agtttcgagg cagagagcaa tacaagagta gattttccta
     9361 ttcctggctc tcctgcaaga agaataacgc tgccgccaac gagtccacca cccagaactc
     9421 tatccagctc acctgtccct atcggtattc tgatattttg cccgaccttt atttcaccca
     9481 gagaaactgg tttttgtttt tcatattctg tcatcgggcc gtggtgagaa attgtctcct
     9541 gatgaaaaga gttccatgcc ccacaggatg gacactttcc aagccatctc actgtggcat
     9601 atccacattc ctggcaggag aaaattttct tttcttttgt cactttacct tcctctgaaa
     9661 agaattttcc atggatgagc cctcaaatcc tgaatcattt caaaaatctg attataaaga
     9721 tcatcttttg caaccagttt tccaactgtg ccttttcctg ccctgatatc tgaaagaata
     9781 gatgctaact gctcgctcgc atatcccaat tttgtaagag caatgtcaag atccaaagat
     9841 gtctgcttca aatttttcat ggtaattttg atatcgttca caatctcagt gtcagaaata
     9901 aggtcggcaa caagcccctg attttctact ttgtcaacaa aattattaat tttagttcct
     9961 gttttcacaa atgtttcact aacattctta atgctggtag aaacttctaa catcgtttgt
    10021 tccagtttat cagttgattg atcaagattt gaaataatat tgttaattct gtcactattt
    10081 gaaagaacaa ttttgtttat ttcctgagac gctgattttg cgcttgtaat aaggtcagaa
    10141 atttcgggtc tgatacctgc tattgttttg ttgagttcca gcgacgtttt attccagttt
    10201 gaggcaagca cattgatatt gtcaattgca aatgatgctt tcaaaagaac ctggtttgtt
    10261 ccctgcacaa tctgaagtaa ttgtttcttt aaattttcat ctgaagtaac attgttcaaa
    10321 gaaaccagca aattgtttac gtttttgaga atatcttctc ctgcgctaag gaatttttca
    10381 agaggaagag gatcttcgcc tttcaaagtt tcccctggct ctataagtgg aacatctttc
    10441 aacccggttg acattatttc catatatttt tcaccaataa tgccatagtt tctcaccata
    10501 aatctgctat gtcttccaag tcgtacttct ggttttattc gaacagtaac aataattctc
    10561 ggctggtcct gttccataac aagatctata cttcttactt ctccaaccct gtaaccactc
    10621 acccttacag gcgctccagg gtcaagcccg ccaatatagt taaaagcaat cttcaattcg
    10681 tacccttttc cacgaagaat tttgccctgg gaaaaaatga acacaaaaaa tatgatcagt
    10741 ccgagaaaaa taaataatcc taccctgaat tgcaaattaa aacttttcgt tttcataggc
    10801 ggctccaata tttagaaaat atttgacaaa ttcatccttt gaatgaagca gttgttccca
    10861 tgtccctgtc tcaataattt ttccttcttt aatcacagcg attctgtctg caagatatct
    10921 tgcaattgaa agatcatgcg ttacaatgag gtctgtcgtt tgaaattcca tgtgaatttt
    10981 cttcattaat ttggtaatac tggcagatgt gatcacatca agccctgctg tgggttcgtc
    11041 gtaaaaaaga acttctggtt cactcatcag cgctcttgcg ataccaaccc ttttccgcat
    11101 accaccactc aattcatctg gcattttgtc cagtattcct gaaagcccta ctgcaagcag
    11161 catctgttct gctttcttac gcacctcttc ttcaggcaat ctcagaaact ccagaaggtg
    11221 aaaaccaaca ttttcccaga tactcattga atcaaacagt gctgcggact gaaacacaac
    11281 tcctgtcttt tttcttaatt ggagcagttc ttcttttgaa atattaaaca tgtctttacc
    11341 cagcacaatc agtttcccat cagatggcgt tgtcaaccca agaagggttt tgagcagcac
    11401 agttttgcca gaaccagaag gaccaagaat gacaactgtc tcattctctg caacattcaa
    11461 actgatctcc ttcaggattt tcatatcctt taaacgcaca acgagatttt cgatttctat
    11521 aaccatttta taaaaaataa aaaattgctg tgagaacaag atttgctaga attacaagga
    11581 agaatgcatg aacaacggct cttgtggttg aaatacccac accctctgcg cctcctgatg
    11641 tctttaatcc ttcaagacag gatgctactg tgataagaat gccaaaaacg aacgctttgg
    11701 caagccccac aagtatatcc ttgagtgtaa tgtattcaaa actctgatac aggtatactc
    11761 ctgatgtgat atgaacaaga tacacagcaa tgatataccc cccaataata cttaaaaagt
    11821 tcgccataat cgttagtatc ggcatcatta ccatagaagc aaacaaacgt ggagtaacaa
    11881 gataggacac aggatcaacc ccaaaaacct caagagcatc aatttgttct gtgattttca
    11941 tgctgccaat ttctgctgcg actgaagcgc caacccttcc cgcgacaata agcgccacga
    12001 taatgggtcc gagttctctt accattgaaa cgctgacaat accacccaca taactttcgg
    12061 caccaaattt tttcaatgtt aaagcgattt ccatcaccat caccatcccc gtaaaagagg
    12121 caatgattcc aacaagagga aatgaatcaa tcccaatttt tgccatctgc tcaaacagta
    12181 gtttttttct tctgaaagca tcagtaaaaa ttttttttat tgcgctggta gtaatgaatg
    12241 taatttcacc aagatagcaa atagcttcaa gggtgagttt tcccagagaa tttatcaacc
    12301 ctatcatgtt ggttgccttt ttgaacagtt ttcaaatctt aaacagtttt tcagaaatgt
    12361 caggtgaaca cggtcgatcg gaccgtttct atgtttcgcg ataataatat cggtcaaccc
    12421 ctttgtttct tctgactcgt cgtattgctc ttctctgtga atgaatatta caaggtcggc
    12481 atcctgttca atagaaccgc tttccctgag atcagcaaga atcggccgtt tatcttctct
    12541 tctttctatt aatctattca tctgagaaag cgctatcaca ggaattgaga
//
LOCUS       DAORYU010000013        12423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_3970_length_12423_cov_5.015766, whole genome shotgun sequence.
ACCESSION   DAORYU010000013 DAORYU010000000
VERSION     DAORYU010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12423
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3970_length_12423_cov_5.015766"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /gene="fmt"
                     /locus_tag="PLW07_00940"
     CDS             <1..833
                     /gene="fmt"
                     /locus_tag="PLW07_00940"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOL49122.1"
                     /translation="GRHLKITPGPIKIWAQNNSILVLQPEKLKGNTEFLNILKSFNPD
                     LFIIISYGKIIPEEYLKIPKIAPINVHPSLLPKYRGSAPIEWALINGERETGVSVIFV
                     TRDVDAGNIISQKSIIIEDQDDIFTLREKLSNIAVEVLLEGFNKLKDGFRGKQQEGKV
                     SYAPKLKKTDGKIVWAGSATSIHNRIRALADWPGTYTFLVSKKGCRMIKIISSIVEKD
                     TGSYGLPGRFVDLNKKILVACGEGMLRIERLQEQGKKQQNALDYLNGHFHLLKDGHLE
                     "
     gene            906..2111
                     /locus_tag="PLW07_00945"
     CDS             906..2111
                     /locus_tag="PLW07_00945"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOL49123.1"
                     /translation="MSIIVQKYGGSSVADTEKIKKVAEKVVNTYRKGNKVVVVVSAMG
                     KSTDNLISLAKQIHDSPPTREMDLLLSTGEIVSVALLCMAIEQLGYEAEGLPGFLAGI
                     RTDSTHTKARICTVDTRRILEELKKGKIVVVAGFQGISAEENITTLGRGGSDLTAIAI
                     AGALKANMCEIYTDVPGVFTTDPNVVPEARVIPVISYQEMLEMASTGAKVMQSRAVEF
                     AQKYHIPFVVKSTFTDSGGTMVTEVDFKEGAVVSSVTLDEKQAKITFFNVPDKPGVAA
                     RIFGALGEASINVDVIVQNVSTSGYTDLSFTVSTSDLEKTFEIAKTIGAELGCKSVTS
                     DSDIAKITVIGLGMMNHPGVAARMFKALAKSKINIEMISTSEIKISCIIRKQDGQKAL
                     KVVHQEFIK"
     gene            complement(2115..3125)
                     /locus_tag="PLW07_00950"
     CDS             complement(2115..3125)
                     /locus_tag="PLW07_00950"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOL49124.1"
                     /translation="MEQVTCILPVAGIGKRLQPHTFTIPKVLLPVAGKPILGYLIDYV
                     KTLGINRFVFITGHLGNKIVDYVKIHHSDVEAIFVRQEEFIGLGYAISLAADHVNGPV
                     FINLGDTLIESNAKDLIEDGISWIALKEVKDPRRFGVAVTDGEGVIKRVVEKPSEMIS
                     TQALCGVYFLNNSHLLFDCLKILISSGRKTRNEFQLTDAIQMMIEKGEKILGIPARIW
                     LDCGKLETLLETNRYLLEKNKPFIPSLPSVVINEPVFIEPSAQLRNCVIGPYVSIGKN
                     VNMVNVIVQNSIINDNASIKNVVLTDSIIGYSAYLTGNLQKINLGDASQVSLMGEEET
                     LF"
     gene            complement(3340..7791)
                     /locus_tag="PLW07_00955"
     CDS             complement(3340..7791)
                     /locus_tag="PLW07_00955"
                     /inference="COORDINATES: protein motif:HMM:NF014852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HOL49125.1"
                     /translation="MYPFIWILLFLFSRNVFGLTLTVDLFPKELKENPKEVIATSVQV
                     APVIDGTLNDSAWDSAAKMAGFWVAGKDESAKNQTVAWVCYDDENIYFAFACIDPRVG
                     LEKIPKDSPSAWQNDCVEIFLSPTGEKEQWYHFILTSGGSQWDDYSDPKYRETSRFGE
                     SWNASWTGATQKTDFGWTAEIKIPFSSMFDTSKYRVVPGWIWRMKLTREDYNPGISEP
                     EFSSYSKIGALFQDILAGLDLIFTTRNFITNPEGKILDKTGQITGWHYYVPAGGKGNM
                     RHVSDSDPVEFTFENADNATIFFGPIKYAPFPASRTVILSAEMKMEAKPDSKSSAVIR
                     YKNPEGKFIQGEGPSGKLRIFPDNEYHVYQIAFPVAAGFAPLHIDISAALYGETVRFY
                     IKSVKLEFGSLVFAEGNDWCLTGNAMGPLSLRNKKIEGCYTYFDAGTDTYFFPRDKKH
                     VSEENTRNNWVPFSEGKLTDGNLSTGVGWPNLWTGSNGIDVVFDLGDFYNVSRVEILS
                     GYDSVAGGQLYVKGPQDLIFVLVDSVFDRVDATKGGPQKKTNHLILKANGSTTRFVRL
                     QFLCNSYNGPAEIQIWGNKMDGRIVPPVAYLQQKGKIVIQNPKEIPLEQGMDTPFLPV
                     PQRFKYGKKEIVLDPSFKIIISPDSTERTKETSDVLKEELFLSTGQNFQKDLFRGSYN
                     TDAQVIFLGICSDRKFLTDVRKIFKDFPAVVEKPEGYVLVAQDKKILVLGADEPGLFC
                     GCMSLLELLKMKNGEWVIPEITISDWPECPYRMMQVRPAPEANLLRALARYKINYVEF
                     AYRYNKKIVELSKMAERYFIKTILGADPRIVLNLTTDPEIIEKSPEEKIEDLGFGRRN
                     ICPSNPKTWELYFKELDQWIDHFTGFLNINFDEMYQTPNGSRWNVCQLCRAKNKHSYE
                     ILADAINTLHRYAAKHKLKLLMLDTCFMGRSISNPDDNDPDWRKALDIIPKDIVMAVW
                     HPADVNELFFSKGFTQVYLSLTGGNWKKRNFPGKYYSGVLWYGLDSLFKVPFVLQESQ
                     ACWSPGKALPGAGDPIDVNINEISLHWEEIVNGSIYPSRIASSKDYFFVDISSVANNS
                     FTDEKPFDGKGWIDFGPNFDLKAMVPGEKMFNGVPFKIIDEKKNNGKSCITVHNRGYV
                     DRTFPFRVEIPVPDKKLISLNFLHTLDNRPGHNYLRKNELAGYYFVVYSDGYYIPVEL
                     KYAVNIANWDGLPCWSYYEPREKSMKGGILCWRGETQSGIEANLYRTEWINPRPDVPI
                     TKIIFSPCWKKSCMNPTLLAITGIEYSEKLKNYVKKIPTETTLKNVDKLNPAFTRGTA
                     IELKGGVLHNDLFYETREKISIRASSVFNNSYREAVLGGEDFKSDVSCIISDSNDFLR
                     FAGKGDVTFSFPEKEILSGVLVSGSYRDERKGEDFPPQKLDLTLYISYDGNNFEKHRE
                     ISDYTPEEEGPKWIEFPEKPIKSFKVEVKAMSPYSMPGLNLVQPYRP"
     gene            complement(7808..9526)
                     /locus_tag="PLW07_00960"
     CDS             complement(7808..9526)
                     /locus_tag="PLW07_00960"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HOL49126.1"
                     /translation="MFNVEDILNKVKKPVTYINNEVNSIHPDIENAQITIALGYPDLY
                     EVGMSNLGIRILYHILNNIEGVACERFFAPGIDLEEILREQGEYLFTLESRLPLKKFD
                     IVGFSISSELNYTNIINLLSLSGIPIYSSQRTDRDPIIIAGGNCSFHPESLSDFIDVW
                     IVGEAEEVMVEFVNVYRQLKGLKRNHIIKELAKIKGCYAPLYYQPQLDESVKPVEAGI
                     PECIERRFIKNFENTPFPVKWVVPLCDIVHDRISLEIMRGCPQACLFCQAGFCWKPVR
                     KRSAETVIKLGLEAYQNTGYEEISLLSFSSADHPEIDQIVTGLTDRLKDKNVAISFPS
                     LRIDTFSFRLANRISQVRRTGLTFAPETGETLRKLIGKPITDDTLIKLAMDAKKTQWR
                     QLKLYFMLGLPGENDNTIEEIETLIKKISKIISVKCSFNVFIPKPHTPFQWEKFPKFE
                     IHAEIKNRLLKNFSKNRFVHLKFHPWEMSAIECLLSRGNRKLGDIIVSVWEAGGKMEN
                     WSETFIFNRWNNAMEKHDLGFEDYLGNSPVICNRWKHIRASLSFDGLHKIWENFNQAL
                     NATDVK"
     gene            complement(9533..10612)
                     /gene="rodA"
                     /locus_tag="PLW07_00965"
     CDS             complement(9533..10612)
                     /gene="rodA"
                     /locus_tag="PLW07_00965"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOL49127.1"
                     /translation="MENYSNPGKVLMILTSVLVALGVLLLYSASRSISTIQTGLPVYI
                     KQMIWVIFGIVIFVWLKDFDYRHWNKLAHVLYLLTLILLVVVLVIGTGRAGRWIKIGW
                     FNFQPSEFAKFVLIIVLARYFAIRDVQRFSTLFGGVLIVGIPFLLILVQPNLGTAFLF
                     IIIFFAMAYLAGAKVKHLLILLAVGILMSPFLWMGLKDYQKQRLVIFMNPWKDPLGKG
                     YNILQSKITIGSGGIIGKGFLKGTQTKLAFLPEFHTDFIFCLLAEEFGLIGVFVLLFL
                     YYVFLTQALRIILTTQDPFGRLVGTGIMVMLVVQIFVNIGMASGILPVAGIPLPFLSY
                     GGSAMLVCFICVATLYNLHKNTTLF"
     gene            complement(10614..12377)
                     /gene="mrdA"
                     /locus_tag="PLW07_00970"
     CDS             complement(10614..12377)
                     /gene="mrdA"
                     /locus_tag="PLW07_00970"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOL49128.1"
                     /translation="MEQRFKNFHQFIKFILGILLIQCINLQVLHFSYYRRLSDQNCIR
                     TIDLGIPRGKIFDRNGRVLAEDKTCFNLVYVPYDLKNPEEVASVLSNILGMDRSELLV
                     QFKKKSSNPFERRILKRFLTQSEIAAVSEYAFKLSGIFVQAGLDRQYTLGEGAAHLLG
                     YTGEVNQEELELLKDEGVKPGHYIGKYGLEKQYDGFLRGKSGGYQIEVDARGHQRKIL
                     KEIAMVPGNNIFLTVDQTVQEACINALGDRSGAIIAMDPRNGEILALVSKPSFDPLNV
                     AKYLQSPYSNKKPFLNRVIAGQYAPGSIFKIITEIAALETGEIGEHDRIECTGSMEVG
                     DRIFHCWKEEGHGWVDINLALPFSCNIFFGTVGAKLGVNKILEYAKLFDFGKPTGIDI
                     PGEKPGYLPSPEESGGPINIAIGQGAISTTPIQLCMMISAVANGGNLWKPFITRKIVT
                     SDEKTIKEFQPVLKKTIFVSDETLTILRRGLKNVVKFGTAAGASVAGIEIAGKTGTAQ
                     IAQRDLGLPTRGAFACYAPADSPSIAMVVVLDEGSSSQAARIAGAIIKKIYTAEEVRS
                     ETTISETQEIQTLPSGSQGFE"
BASE COUNT         3803 a         2653 c         2194 g         3773 t
ORIGIN      
        1 aaggaagaca tctgaaaatt acccctggcc caataaaaat ctgggctcaa aacaatagta
       61 ttttggtttt gcagccagaa aaattaaaag gcaatactga atttttgaat attttaaaat
      121 cctttaaccc tgacctgttt attattattt catatggaaa aatcattcct gaagaatatc
      181 tcaaaatacc aaaaattgct cccattaatg tgcatccatc tcttcttcca aaatatcggg
      241 gatcggcacc aattgaatgg gctctcatta atggcgaaag agaaacagga gtttcagtaa
      301 tatttgtcac ccgcgatgta gacgctggaa atatcatcag ccagaaatca ataatcattg
      361 aagaccagga cgacattttt acgctgaggg aaaaattatc aaacatagca gttgaggttc
      421 tccttgaagg ttttaacaaa ctgaaagatg gattcagggg aaaacaacag gaaggaaagg
      481 tttcttacgc tccaaaactc aaaaaaactg atggaaaaat tgtctgggct ggcagcgcaa
      541 cgtcaattca taatagaatc cgcgctctcg ctgactggcc aggaacatat acatttcttg
      601 tttcaaaaaa gggctgcaga atgataaaaa ttatctcgtc tattgtagaa aaagatactg
      661 gcagttatgg actccctggt agatttgttg atctaaacaa aaaaatcctt gttgcctgtg
      721 gcgagggaat gttgagaatt gaacgacttc aggaacaggg taaaaaacag caaaatgcac
      781 ttgattatct caatggtcat tttcatcttc tcaaagatgg gcatcttgaa tagacccatg
      841 tttattgaaa aacccatttt tgtcgtgtaa aatattatgc taaaaatatt tttgaaggag
      901 gatggatgtc gattatcgtc cagaagtatg gcggaagttc ggttgcggat accgaaaaga
      961 taaaaaaggt tgctgaaaaa gttgtaaata catacagaaa gggcaacaaa gtggtcgtcg
     1021 ttgtgtcagc catgggaaaa tcaacagaca accttattag ccttgcaaaa caaattcatg
     1081 attctccgcc aacaagagaa atggatttgt tgctttcaac aggagaaatc gtttcagtag
     1141 cacttttgtg catggcaatt gaacaactgg gatatgaagc agaaggactt cctggatttc
     1201 ttgcaggtat aagaacagat agcacccata caaaagcgag aatctgcact gttgatacgc
     1261 gaaggattct tgaagaacta aaaaagggaa agatcgttgt ggtagcaggc tttcagggaa
     1321 taagcgccga agaaaatatc acaacccttg gcaggggcgg ttcagacctg actgccattg
     1381 cgattgcagg cgcattaaaa gcaaatatgt gcgaaatata tactgatgtt cccggtgttt
     1441 tcacgactga tccaaatgtt gttcctgaag cccgcgtaat tccggtcatt tcctatcagg
     1501 aaatgcttga aatggcttca acaggtgcaa aggtaatgca atcgcgcgcc gttgaatttg
     1561 ctcaaaaata tcatatacca tttgttgtca agtcaacatt tactgacagt ggaggcacta
     1621 tggtcactga ggttgatttc aaagaaggtg ctgtggtatc atccgtaacc cttgacgaaa
     1681 aacaggcgaa aattacgttt tttaatgtgc cggacaaacc cggtgttgct gccagaattt
     1741 tcggtgctct tggagaagca agtatcaatg ttgatgtcat cgtgcaaaat gtcagtacca
     1801 gcggctatac tgacttgtcc tttacagtaa gtacttctga ccttgaaaaa acatttgaaa
     1861 ttgcaaaaac cattggtgct gaattaggat gcaagtcagt tacatctgat agcgatattg
     1921 caaaaatcac tgtgatagga cttggtatga tgaaccatcc gggagtcgcg gcaagaatgt
     1981 ttaaagcact tgcaaaatct aaaattaaca ttgaaatgat cagtacctct gaaataaaaa
     2041 tatcatgtat catacgcaag caggatggtc aaaaagcatt gaaggtggtt catcaggaat
     2101 ttatcaaata aactttagaa aagtgtctct tcttcaccca tcagactgac ctgagaagca
     2161 tctccaagat ttatcttttg aaggtttcct gtgagatatg cagaatagcc aatgattgaa
     2221 tcagtcagga caacattttt gatgctggcg ttgtcattga tgatactgtt ctgaacaata
     2281 acattgacca tgttaacatt tttcccgatt gaaacatagg gtccaatgac acaattcctc
     2341 aattgggcag agggttctat aaaaacgggt tcatttatta ccacactcgg taatgacgga
     2401 ataaaaggtt tgtttttttc aagcaagtat ctatttgttt cgagaagggt ttcaagttta
     2461 ccacaatcca gccagattct ggccgggatt ccaagaattt tttcgccttt ttctatcatc
     2521 atctggatgg cgtcagtgag ctgaaattca tttcttgttt ttctccctga actaatcaat
     2581 atcttaagac aatcaaaaag aagatggctg ttgttcaaaa aataaactcc gcacaatgcc
     2641 tgtgtggaaa tcatttctga tggtttttca accactcttt ttataacacc ttcgccatct
     2701 gtcaccgcaa ccccgaatct acgcgggtct ttcacttctt tcaaggcaat ccagctgata
     2761 ccgtcttcaa ttaaatcttt tgcattggat tcaattaatg tatctccgag attaataaaa
     2821 acaggtccat tcacgtggtc tgctgcaaga gaaattgcgt aacccagccc gataaattct
     2881 tcctgtctaa caaaaattgc ttccacatcc gaatgatgaa tctttacata atctacaatt
     2941 ttatttccaa ggtgtccggt gataaaaacg aatctgttta tgcccagtgt tttcacataa
     3001 tcaattaaat agccgagaat tggcttgccc gcaacaggta aaagcacttt tggtattgta
     3061 aaggtatgcg gctgaagtct ctttcctatc ccggcaaccg gaaggatgca ggttacttgt
     3121 tccatactgt tattatatca tgaaaaatag aaaaaacttg aaaaaaacac ccaaacactg
     3181 gagatttact tgcatatgcc agaataagac tctgggacgc aacaggttgt tatccgatga
     3241 gaacactcag aaatggctgg aagtataata tctggaagca attaattcca gatattcagg
     3301 aagtataatg taaaaagttc tcttttattt caagtcaatt cacggtctgt aaggttgaac
     3361 aagattcaaa cccggcattg aatacggaga cattgctttc acttccactt tgaaagattt
     3421 tatcggtttt tcgggaaatt caatccattt tgggccttct tcttcaggag tgtaatcaga
     3481 aatttctctg tgtttttcga aattgtttcc atcgtaagaa atatagagag tcaaatcaag
     3541 tttttgaggg gggaaatctt caccttttct ttcatctctg taacttccag aaaccagcac
     3601 tcctgacaat atttcctttt ctggaaaact gaaggttaca tctccttttc cagcaaacct
     3661 gaggaaatcg tttgaatcag atatgatgca gctgacatca cttttaaaat cttctccacc
     3721 cagtactgct tctctgtagg aattattaaa aaccgagctc gccctgattg aaatcttttc
     3781 tctggtttca tagaaaaggt cgttgtgcaa caccccaccc tttaattcta tagccgtacc
     3841 ccgagtaaag gcagggttca gtttatccac atttttcaat gtggtttctg tcggaatttt
     3901 tttaacataa tttttcaatt tttcagaata ttcaatacca gtgatggcaa gcagtgttgg
     3961 attcatacag gatttcttcc agcaagggga gaaaataatt tttgttatcg gaacgtcagg
     4021 tctgggattt atccattctg tacgataaag gtttgcttca atccctgatt gtgtttctcc
     4081 cctccagcat aaaatacctc ccttcatgga tttttcgcgc ggttcatagt aactccagca
     4141 aggcaaccca tcccagttcg caatgtttac agcatatttc aattcaacag gaatgtagta
     4201 accgtcactg taaacaacaa aataatatcc tgcaagttca ttcttcctta aatagttatg
     4261 acctggcctg ttatcaagcg tatgaagaaa attcaaagat atcaattttt tatcaggcac
     4321 cggtatttct actctgaatg gaaatgttct atcaacatat cccctgttat gcacagtaat
     4381 acaggatttt ccattatttt tcttttcgtc aataattttg aacgggactc cattgaacat
     4441 cttctcaccc ggcaccattg ctttcaaatc aaaatttggg ccaaaatcta tccacccttt
     4501 tccatcaaat ggcttttcat cagtaaaaga attgttggca acagatgaaa tatcaacaaa
     4561 gaaataatcc ttgcttgaag caattctgga gggatatata gaaccgttta ctatttcttc
     4621 ccagtgcagg gaaatttcat taatattgac atctatcgga tctcctgcac caggcagtgc
     4681 tttccctgga gaccagcatg cctgagattc ttgaagtaca aacggaacct taaaaagact
     4741 atccagacca taccataaaa caccgctata atacttaccc gggaaattgc gtttcttcca
     4801 gtttcctcca gtcaatgata aatacacctg agtaaaaccc ttactgaaaa aaagttcatt
     4861 tacatctgct ggatgccaga cagccatcac aatatctttt ggtataatat ccagggcttt
     4921 cctccaatca ggatcattat cgtctggatt gctgatgctt cttcccatga aacatgtatc
     4981 cagcatcaac agtttcaatt tatgctttgc cgcgtatcga tgcagggtgt taatggcatc
     5041 tgcaaggatt tcataactgt gtttgttttt tgcgcgacac aactgacaaa cattccacct
     5101 tgaaccgttt ggtgtctggt acatttcatc aaaattgatg ttcaaaaatc ctgtaaagtg
     5161 gtcaatccac tgatcaagtt ctttaaaata caattcccac gttttcggat ttgaaggaca
     5221 aatatttctt cttccaaaac ccagatcttc aattttttct tcaggagatt tttcaataat
     5281 ttcggggtcc gtggttaaat taagaactat tctcggatca gctcccagga ttgttttgat
     5341 aaagtatctt tctgccattt tgctcagttc aactattttc ttgttatatc gataggcaaa
     5401 ctcaacatag ttaatcttgt atcttgccag ggctcgcaga agatttgctt ctggcgcagg
     5461 tctgacctgc atcatcctgt aagggcattc tggccaatcg ctgatcgtaa tttcagggat
     5521 tacccattct ccatttttca tcttcaacag ttccagcagg ctcatgcaac cacaaaatag
     5581 tcctggttca tctgcaccca gtaccagaat ttttttgtcc tgcgcaacca gaacataacc
     5641 ctcgggcttt tccaccacag caggaaaatc cttaaaaatt ttcctgacat cagttagaaa
     5701 ttttctgtct gaacaaatac caagaaaaat tacctgcgcg tcggtattgt acgaaccacg
     5761 aaataaatcc ttctgaaaat tctgccctgt cgaaagaaat aattcttctt tcaaaacatc
     5821 agatgtttct ttcgtccttt ctgtgctatc aggtgatata ataattttga atgatgggtc
     5881 aagaacaatt tcttttttcc cgtatttgaa cctctggggc acaggtaaaa aaggtgtatc
     5941 catcccctgt tcaagtggaa tttccttcgg attttgaatc acgattttac ccttctgctg
     6001 aagatatgcc actggaggta caatcctgcc atccatttta tttccccaga tttgaatttc
     6061 agcaggtcca ttgtaactat tacacaaaaa ttgcaacctg acaaaccttg ttgttgaacc
     6121 attcgctttt aaaatcagat ggtttgtctt tttttgggga ccgccttttg tagcgtcgac
     6181 tctgtcaaaa acactgtcaa ccagaacaaa tattaaatcc tgtggtcctt tcacatataa
     6241 ttgtccgcct gcaacactgt cataaccact caatatttca acccgtgaga cattataaaa
     6301 gtcaccaaga tcaaacacta catctattcc atttgaccca gtccataaat ttggccaacc
     6361 cacgcctgtt gatagattgc catcagtaag tttcccttca gaaaaaggta cccagttatt
     6421 tcgggtattc tcttcagaaa catgtttttt gtccctcggg aaaaaatagg tatctgtacc
     6481 cgcatcaaaa tacgtgtagc atccttcaat ttttttgttc cttaaactta atggtcccat
     6541 tgcattacct gttaagcacc aatcatttcc ttcagcaaaa accagactgc caaattccag
     6601 ttttacgctt tttatataaa acctcactgt ttctccgtaa agagccgcac taatatctat
     6661 gtgcagaggt gcaaagccag cagcaacagg aaaagcaatc tgatacacat gatattcatt
     6721 gtcaggaaaa attctcaatt ttcctgatgg accttcaccc tgtatgaatt tgccttcagg
     6781 atttttatat ctgataactg cagaactttt tgaatctggc tttgcctcca ttttcatttc
     6841 agcacttaaa atcacggttc tgctggctgg aaatggagca tatttaatgg gtccgaagaa
     6901 aatcgtcgca ttgtcagcat tttcaaacgt aaactccact ggatctgagt cagaaacatg
     6961 tctcatattc ccttttccac cagcaggaac atagtaatgc cagccagtga tctgccctgt
     7021 tttatcaagg atttttcctt ctggatttgt aatgaaattt cttgtggtaa aaatcaagtc
     7081 aagacccgcc agtatatcct gaaataaagc acctattttt gagtaacttg aaaattcagg
     7141 ttctgaaata ccaggattgt aatcttctct ggtaagcttc atcctccaga tccagccagg
     7201 aacaacacga tattttgaag tatcaaacat ggaagaaaac ggtatcttaa tttcagcggt
     7261 ccagccaaaa tctgttttct gtgtcgctcc tgtccacgat gcgttccagg attcaccaaa
     7321 acgactggtt tccctgtact ttggatctga ataatcatcc cattgactac ccccgctggt
     7381 caaaatgaag tgataccatt gttctttttc ccctgtgggt gaaagaaaaa tctccacgca
     7441 atcattctgc caggcagagg gactatcctt tggaattttt tcaagcccaa ctctgggatc
     7501 aatgcaggca aaagcaaaat aaatattctc atcatcataa caaacccacg caactgtctg
     7561 attctttgcg gactcatcct ttcctgcaac ccagaatcca gccatctttg ctgcactatc
     7621 ccaggcagaa tcatttaatg taccgtcaat aactggcgca acctgaacag atgttgcaat
     7681 tacctctttc ggattttcct tcaattcttt tggaaataaa tcaactgtca gcgtaagtcc
     7741 aaagacattt ctggagaaaa gaaataataa aatccagata aaaggataca taggacctcc
     7801 tttctggcta ttttacatct gtagcattta gtgcctgatt aaaattttcc caaatcttat
     7861 gaagaccgtc aaatgaaaga gatgccctga tatgtttcca tctgttacat atcacaggtg
     7921 agtttccaag ataatcttca aaacccaagt catgtttctc cattgcgtta ttccagcgat
     7981 taaaaataaa tgtttcagac cagttctcca ttttaccgcc tgcctcccac acagaaacaa
     8041 taatatctcc aagttttcta tttcctctag aaagaagaca ttctatcgca ctcatttccc
     8101 acggatgaaa tttcaaatgc acgaatctat tttttgaaaa atttttcagc aacctgtttt
     8161 ttatttccgc atgtatttca aacttaggaa atttctccca ttgaaacgga gtatgtggtt
     8221 ttggaataaa aacattaaaa gaacacttta ctgaaataat ttttgagatt ttttttatca
     8281 acgtctcaat ttcttcaatt gtgttatcat tttcaccagg tagccctaac ataaaataca
     8341 gtttcaactg acgccactgc gttttttttg catccatggc aagttttatc aacgtatcat
     8401 ctgtgattgg tttccctatc aattttctaa gggtttctcc ggtttctggt gcaaatgtca
     8461 gccctgtcct tctcacctgg ctgattctgt ttgcaagacg aaaagaaaat gtgtctattc
     8521 tcaatgaagg aaaagaaatt gcaacatttt tgtctttgag tcggtcagtc aatccagtca
     8581 caatttgatc tatctctgga tggtcagcag atgaaaacga cagaagagaa atttcttcat
     8641 aaccggtatt ctgatatgct tcaagtccaa gtttgataac cgtttccgca cttcttttcc
     8701 tcacaggttt ccagcaaaat ccggcctggc agaaaagaca cgcctgcggg catcctctca
     8761 tgatttcaag tgaaatccta tcgtggacta tgtcacataa aggtacaacc catttcacag
     8821 gaaatggcgt gttttcaaaa ttttttataa aacgtcgttc tatgcattca ggaatacctg
     8881 cttcaacggg cttcacactt tcatcaagtt gcggctgata atataaaggt gcataacatc
     8941 ctttaatttt tgcgagttct ttgatgatat gatttctttt gagccccttc aattgtctgt
     9001 aaacattaac gaattcaacc atgacttctt cggcttcacc cacaatccag acgtctatga
     9061 aatcagaaag agattctgga tgaaacgaac aattaccgcc agcgataata atcgggtccc
     9121 tatcggttct ctgtgacgaa taaattggaa tacccgatag agataaaagg ttaatgatat
     9181 tggtatagtt caattcagaa gaaatactga aaccaacgat atcgaatttt tttagtggta
     9241 accgtgattc aagtgtaaac aaatattcac cttgttcccg caggatttct tccaggtcaa
     9301 ttccaggagc aaagaacctc tcacaggcaa caccttcaat gttattgaga atatgataca
     9361 aaattctaat tccaaggttt gacatgccga cttcgtacaa atcaggatat cctaaagcaa
     9421 ttgttatctg cgcattttca atgtcaggat gaatggaatt tacttcattg ttgatgtatg
     9481 taactggctt ttttacctta tttaaaatgt cttcaacgtt aaacatggaa tatcaaaaaa
     9541 gagtggtatt tttatgcaga ttgtaaagtg tggcaacgca tataaaacat acaagcatcg
     9601 cagaaccgcc gtaacttaaa aatggcaatg gaatcccggc cacaggcagg attcctgaag
     9661 ccatacctat attgacaaaa atctggacaa caagcatcac cataatacca gtgccaacaa
     9721 gtcgtccaaa tggatcctgt gttgtgagta taatcctcaa cgcctgagtc agaaaaacat
     9781 aatagaggaa tagaagaaca aaaactccaa tgagcccgaa ttcctctgca agtaaacaga
     9841 agataaaatc agtatgaaac tctggaagaa aggcaagttt tgtctgggtt cccttgagga
     9901 agccctttcc gataatacca ccagaaccaa tggtaatctt tgattgaagg atattatatc
     9961 ctttaccaag gggatccttc cacggattca taaaaatcac aagcctttgc ttttgataat
    10021 cttttaatcc catccacaaa aatggactca ttaatatccc gacagccaat aaaattaaaa
    10081 gatgttttac ctttgctcca gcgagatatg ccattgcaaa aaatataata ataaaaagaa
    10141 atgctgttcc aagatttggc tgaacaagaa taagtaagaa gggaatacct acaatcaata
    10201 cacccccaaa aagtgttgaa aacctctgaa catctctaat tgcaaaatat cgtgccagca
    10261 ctataatgag tacaaatttt gcaaattctg acggctgaaa attgaaccac cctattttta
    10321 tccatcttcc agcccttcct gtccctatta caaggactac caccagaaga atcagggtga
    10381 gtaagtataa tacatgggca agcttattcc agtgcctgta gtcaaaatcc tttaaccaca
    10441 caaaaattac aataccgaat atcacccata tcatttgttt gatataaaca ggtaaacctg
    10501 tttgaatcgt tgatatactt cttgaagcgc tatagagtaa taacaccccg agtgccacaa
    10561 gtactgaagt cagaatcatc aaaaccttcc cgggattgct ataattttcc attttattca
    10621 aacccctgac ttccagacgg taatgtctgt atttcttgag tttcgctaat tgttgtttcg
    10681 cttctcactt cttcggcagt atagattttc ttgataatgg ctcctgctat tcttgctgcc
    10741 tgagacgagc ttccttcatc aagaaccacc accattgcga tactcggact gtcggcaggc
    10801 gcgtaacagg caaaagcacc ccttgtgggc aatccaagat ccctttgtgc aatctgggct
    10861 gtccctgttt taccggcaat ttcaataccc gcaacactgg cacctgctgc ggttccaaat
    10921 tttacaacat tttttaatcc ccttctcaat atcgtcaggg tttcatctga aacaaaaatt
    10981 gtttttttaa gaacaggctg aaattccttt atcgtcttct catcagatgt gactattttc
    11041 ctggttatga atggtttcca cagattacct ccatttgcaa ccgctgatat catcatacat
    11101 aattgaatgg gtgtcgtcga aatagcaccc tgccctattg caatattgat cggcccaccg
    11161 gattcttctg gagaagggag atatcctggc ttttctcctg ggatatcaat acctgtaggt
    11221 ttgccaaaat caaaaagttt agcatattca agaatcttgt taacgccaag ttttgctcca
    11281 acagttccaa aaaatatatt gcaggaaaat ggaagagcaa ggtttatatc tacccacccg
    11341 tgtccttctt ctttccagca gtgaaaaatt ctgtctccaa cttccatgct gccagtgcac
    11401 tcaatacggt cgtgctcacc aatttcacca gtttcaagag ccgcaatttc ggttataatt
    11461 ttaaagattg aacccggagc atattgcccc gcaattacac gattaagaaa cggtttttta
    11521 ttactgtatg gagactgcag atattttgca acattcaagg ggtcaaaaga aggtttactg
    11581 acgagtgcaa gaatttcgcc atttctcggg tccatcgcaa taatcgcacc ggacctgtca
    11641 ccgagagcat taatacatgc ctcctgcaca gtctggtcca cagtaagaaa aatattgttt
    11701 cctggtacca tggctatctc tttcagtatt tttctttgat gacccctggc atcaacttct
    11761 atctggtatc ctcctgattt ccccctcaaa aacccgtcat actgtttttc caatccgtat
    11821 tttccaatgt aatggcctgg ctttacgcct tcatctttca acaattcaag ttcttcctga
    11881 ttcacttccc cggtataacc aagtaaatgc gcagcgcctt ctccaagagt atattgtcta
    11941 tccaatcctg cctgcacaaa aatacctgat agtttaaaag catattctga aacagccgca
    12001 atttctgatt gagtcaggaa tcttttcaaa atccttcttt caaaaggatt tgatgacttt
    12061 ttcttaaatt gaaccagaag ttctgaacga tccataccaa gaatgttaga aagaacagag
    12121 gcaacttctt cgggattttt aaggtcatat ggcacatata caagattgaa acatgtctta
    12181 tcttcagcaa gcaccctgcc atttctgtca aaaatctttc cacgaggaat tccaagatca
    12241 atggtcctga tgcaattctg gtcagacagg cgtctatagt acgagaaatg aagaacttga
    12301 agatttatac attgaatcaa caaaatcccc agtataaatt ttatgaattg atgaaaattt
    12361 ttgaatcttt gctccataga attaaactca ggttactgaa agataccctt taagaagatt
    12421 ata
//
LOCUS       DAORYU010000014        12116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_4066_length_12116_cov_5.259182, whole genome shotgun sequence.
ACCESSION   DAORYU010000014 DAORYU010000000
VERSION     DAORYU010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12116
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4066_length_12116_cov_5.259182"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..1470
                     /locus_tag="PLW07_00975"
     CDS             193..1470
                     /locus_tag="PLW07_00975"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOL49129.1"
                     /translation="MAILAINGGKKAVTKQPKISCLFQRMLQEQESLIVELVRKREIS
                     FSPVVKELEEKFAAYTGTKYALTTNNGTAALHSAFFSVIKNPGDQVITSVHTWHLGVT
                     PIICTGGVPVFCDIEPLTLNIDPSKIEEKITPKTVAICVTHVYGHPCDMDAINSVAKK
                     HGLAVIEDASHSHGCEYKSKKTGSLGDIGCFSLQGSKLMSGGEAGIIVTDNHDYYQSC
                     IMLGHYERMEKLSAKYDRYKANQEIPFMNLGYKYRIHPFAAAMALVELKYLDERNQLQ
                     IQNCSYIGRNLEKIEGFKGPFVAPYATKLGWLNFLIRLEPEYFKGIPRTKIIDALRAE
                     GIEAATGRPGYIPLVRQPLLNEIEHHPAYVIWYHFLNNHPEYKIDDYPVAEKVLFERI
                     AIPSMRDIEYDQEYLQQICDAFRKISQHKDELK"
     gene            1467..2237
                     /locus_tag="PLW07_00980"
     CDS             1467..2237
                     /locus_tag="PLW07_00980"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOL49130.1"
                     /translation="MMEIIDCHIHPAVDRQTDFCFYGSPGSIKKQFEDLKRAGISKAC
                     GSVIVAKTPSVFDEVKKLNDFALSIRDRYPDFYIPGIQIHPSFPDESCREIERCCTQH
                     QVTWIGELVGYLMGFPDDYASKSMIKIMKFASQYKVVVNFHCGDMKIVEKLCKSVPEM
                     NFVLAHPGEREHFLSRINKVAELKNLYLDISGTGIDRYRMLKKAVDIAGYKKLLFGSD
                     YPINNPAVYIHGVLFEKLSEKATQSMFADNFTRLIKRG"
     gene            2240..3082
                     /locus_tag="PLW07_00985"
     CDS             2240..3082
                     /locus_tag="PLW07_00985"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOL49131.1"
                     /translation="MFRKLCVLILLVVATGYCSEFSHLEPLGIQYSYHYLDTPRPIRI
                     HVLKIDLTKNKVVPLVIVAKDPDGKGPAEAVLTNPFALVGCMDDIVAFVNTNPWDSFP
                     DEKGEKNTHWYEGQHVNIFGLVATGGKIISPAGDGCISVWSDTSGKINISKFPDTDTL
                     VEGVAGWYQIVKDKTIIPKQTNQLNPLTGIGTDATGYIMWLVVADGRQKGFSEGMSHY
                     ELAEFMLKLGCEQVALMDGGGSSIMAIRDKNGRWQVVNSPSDRILFIKKIRPVPVILG
                     IKKK"
     gene            complement(3084..6308)
                     /gene="carB"
                     /locus_tag="PLW07_00990"
     CDS             complement(3084..6308)
                     /gene="carB"
                     /locus_tag="PLW07_00990"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007786109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOL49132.1"
                     /translation="MPPRHDIKSVLIIGSGPIVIGQACEFDYSGTQACKALRHLGYRI
                     ILVNSNPATIMTDPEMADVTYIEPLTPEAIEKIIEKEKPDALLPNLGGQTGLNISSVL
                     HKTGILKKFGVKIIGVEADAIERGEDRIVFKETMKMLGIPMPESDPAYSIEEAEKIAD
                     RLGYPVVIRPAYTLGGTGGGLVYNRDELKVVAARGLAASLVGQILIEESVIGWEELEL
                     EVVRDSKNQMITVCFIENVDPMGVHTGDSFCVAPMLTIDEKLQQRLQEYSFRIVEAIK
                     VIGGTNIQFAHDPKTDRVVVIEINPRTSRSSALASKATGFPIALISAKLAAGLTLDEI
                     PYWKEGTLEKYKPSGDYVVVKFARWAFEKFKGVKDELGTQMRAVGEVMSIGKTYKEAL
                     QKAIRSLETGRYGFGFVKDFNKKSLQELLEMLSYPTSERQFVMYEALRKGATIDQLYE
                     KTYIKPWFIQQMKELVELEEEILKHRGKAIPDKLLTQAKKDGFSDKYLAEILGVSEQD
                     IRNRRTQLKIVQSWDAVPVCGVEDASYFYSTYNGPDKIKVSKRKKVIILGGGPNRIGQ
                     GIEFDYCCVHAAFALKEMGYETIMINCNPETVSTDYDTSDRLYFEPLTVEDVVSIYQK
                     EKPEGVIVQFGGQTPLNIARQLESEGVRILGTTVDTIDLAEDRDRFRKMMETNGIPMP
                     ASGMASSEKEAVKIAEQIGYPLMVRPSYVLGGRGMEVVYDEQMLREYVSAAVGITPER
                     PILIDKFLENAVEAETDAISDGTDCIIPAVMEHIEQAGIHSGDSACVIPPTGIPAKHL
                     ETIYHYTETIAKTLKVVGLINVQYAICDDKVYILEANPRASRTVPLVSKVCNIPMAKI
                     ATKLLVGEQLNSLNVVRRKLSHFGVKEAVFPFNMFPEVDTLLGPEMRSTGEVLGMADT
                     FGTAFYKAEEAAGQRLPLQGAVLITVADREKQFIVPVAKKFHALGFKIYATTGTANIL
                     KNHGIPCDIAWKVHEQRPNIVDMIKNGQIHVIINTPAGKQSQYDDSYIRKNAIKYRIP
                     YMTTIAAAHAAAKGIEAMIKSGSSYSVKSLQEYHKEIADSPIEH"
     gene            complement(6353..6619)
                     /locus_tag="PLW07_00995"
     CDS             complement(6353..6619)
                     /locus_tag="PLW07_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49133.1"
                     /translation="MANVRFMLAVVKGREGVNHPGLCMVVDSEKWFVFNDMLGFCFRR
                     TTETGTEDIPVDEMKRKYAGIYRLIAAKWSQLTALLKGEQTVDI"
     gene            complement(6628..7791)
                     /locus_tag="PLW07_01000"
     CDS             complement(6628..7791)
                     /locus_tag="PLW07_01000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOL49134.1"
                     /translation="MTEIDFRKLVVSIEEDVIAWRRHFHKFPELSNKEVQTSHFIQNL
                     LEKMGIPYRRAAGTGIIAEIAGTGNRLIGLRADMDALPVQEETGLEFSSVYPGVMHAC
                     GHDAHMAIALGVAKIFSQIKSIPFSIRFLFQPSEESMPCGAPGMIKEGALDGMKHLLG
                     FHVWAGLDTGEFSVNKGPVMASADRFTIFIKGKGGHGSSPHKAIDPVVAAAFFITQLQ
                     TIVSRQSDPQNPLVISVGKITAGDAFNIIPEQVELLGTVRTYDKNQNMLAKKSMKRML
                     KGLEDGFGVKTEIHYEEFVPATYNNPSLSEKVITILSSCFGNRAVKIDEKPTMGSEDF
                     SFYSQKIPSCYINIGCGKSPFFHHSNKFNLDERCLAIGVEAMATVIRYTGLCK"
     gene            complement(7810..9081)
                     /gene="serS"
                     /locus_tag="PLW07_01005"
     CDS             complement(7810..9081)
                     /gene="serS"
                     /locus_tag="PLW07_01005"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004888482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOL49135.1"
                     /translation="MFPIKFVRENLDLIKKAAELKNITVDWASFQNLEQQRRQMIQDL
                     EEKKRLHKKLSTDLAQMKKNGQDANHLLLQAKQISSEIKEKEDCLKEIEAQIDTTLAS
                     IPNIPHPSVPAGRTSNDNVVVRTWGEIKQPSFEIIPHYEIGRNLGVLQLERAAKMTGS
                     FFPAFSGQGALLVRALINFMLDLHRKNGFVELWPPALVNRASMFGTGQLPRLEDDMYR
                     LKEEDLFLIPTAEVPVTNYHREETIPEDTLPIRYCAYSPCFRREAGAYGSDTRGLIRL
                     HQFDKVELVIFSHPQHSYDEHEQLVQEAEKVLQMLGLAYRVVLLCTGDTSFAAAKCYD
                     LEVWSPGVKKWLEVSSCSNFEAFQARRANIRYRSASGKTDYVHTLNGSGIALPRTIIA
                     IMENYQTEKGTIIIPEVLRPYMGGMTEISLD"
     gene            complement(9068..9316)
                     /locus_tag="PLW07_01010"
     CDS             complement(9068..9316)
                     /locus_tag="PLW07_01010"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOL49136.1"
                     /translation="MILVSGTIVILYALQQYYVQKSKLLMYQQEIKKLTQENAMLEKT
                     LKQAETPFMTEKIAREQLGMMKPGEYKVQIKEGESVSH"
     gene            complement(9393..10130)
                     /locus_tag="PLW07_01015"
     CDS             complement(9393..10130)
                     /locus_tag="PLW07_01015"
                     /inference="COORDINATES: protein motif:HMM:NF024775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HOL49137.1"
                     /translation="MTFTDGRRRAERFVKQMCGKDVWTWRQLKIQTITAGKEQYVICP
                     DFLTSRSIVYSAGIGDDISFDLDIIKNFGAHVFGFDPSPVSIAWIEKYNLPLEFRFFP
                     YGISDHDGEMLMFPPENPNSTSFPLARKTSVEPFEVCVMRLATIMKELGHVRIDLLKL
                     DIEGGEYAVIADMVESDIRPGQVVVEFHHRFPQIGISKTKTALRQLKQAGYKIVHIES
                     RGYVYSFVHRHLLSLKKEGVLVVKPEI"
     gene            complement(10130..10696)
                     /locus_tag="PLW07_01020"
     CDS             complement(10130..10696)
                     /locus_tag="PLW07_01020"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOL49138.1"
                     /translation="MNILALTGSRNPEGKTGLCVKALFDGAGKDHQCEQIFLTAKKIE
                     RCIQCDFNGWGKCRTEGICTIEDDFHSIVEKIRQADAIVFATPVYFSDISESMKSFLD
                     RLRRITRHQAGKTGIEGKPAIGICVAGGGGGGAVMCCFFLERILTGCGFVVVDMIPCR
                     RQNLQHKSQILGLTGKWFVEHITTEGYK"
     gene            10852..10923
                     /locus_tag="PLW07_01025"
     tRNA            10852..10923
                     /locus_tag="PLW07_01025"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10884..10886,aa:Phe,seq:gaa)
     gene            11041..>12116
                     /locus_tag="PLW07_01030"
     CDS             11041..>12116
                     /locus_tag="PLW07_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOL49139.1"
                     /translation="MNELVEILKKVNALESEIQKYPLDQLRAKTGYFKERLSKGESLD
                     SILPEAFAVVRETARRVINMRHFDCQIVGGVVLHRGNVAEMATGEGKTLVATLPLYLN
                     ALTGRGCHLVTVNDYLAKRDRFWMGPVFEALGLSVGLIQHDMVPHERQKAYHADITYG
                     TNNEFGFDYLRDNMAWRLEDQVQRPLYYAIVDEVDSILIDEARTPLIISGPSEESVSL
                     YYDIDRVVRRLKKGEDYTVDEESQTASLTETGVAKCEKMLNVTNLYDISNTEIVHHII
                     QSLRAHNFYHRDDQYIVKEGKVTIVDEFTGRLMPGRRWSDGLHQAVEAKEGVQIESEN
                     QTLATISFQNYFRLYEKIAGMTGTA"
BASE COUNT         3398 a         2661 c         2373 g         3684 t
ORIGIN      
        1 ggaatttgaa caaaaagaga taaaacaatt acctttaacc aggaccctaa attctacctt
       61 ttttgctctt gacaaatggg gggcagtaca atattacccc ccgaggtttc tgtcaagagg
      121 tgaaatctgc tggaaccaga gcagataagg ttcattgata ctgtgttata atttatggaa
      181 aaaggcggtt ttatggcaat cctggcaatc aatggtggaa aaaaggcagt tacaaaacaa
      241 ccgaaaattt cctgtctttt tcagcggatg cttcaggaac aggaatctct gattgtggaa
      301 ctggtcagaa aaagagaaat ttcgttttcc cctgttgtga aagaacttga agaaaaattt
      361 gcggcataca caggcacaaa atacgctctt actactaata acggaactgc agcacttcac
      421 agtgcatttt tttctgtcat caaaaatcca ggggaccagg tcattacaag tgttcataca
      481 tggcatcttg gagtaacacc catcatatgt actggcggag tacctgtgtt ctgcgatatt
      541 gagccactta cattgaatat tgacccatca aaaattgaag aaaaaatcac tccaaaaact
      601 gttgcaatat gtgtcacgca tgtgtacgga catccctgcg atatggatgc catcaattca
      661 gttgcaaaaa aacacggtct tgcagttata gaagatgcct cacattctca tggatgcgag
      721 tataagagta aaaaaacagg cagtttaggt gacattggtt gcttcagttt gcagggaagc
      781 aaactgatga gtggtggaga agcaggtatc attgtcacag acaatcacga ttactaccaa
      841 tcgtgtatta tgctcggtca ttacgaaaga atggaaaaac tgtctgcaaa atatgacagg
      901 tacaaagcaa accaggaaat tccttttatg aacctcggtt ataaatacag aattcatccg
      961 tttgctgctg caatggcact tgtagaacta aagtatcttg atgaaaggaa ccaattgcag
     1021 atacaaaact gttcgtacat tggtaggaat cttgaaaaaa ttgaaggatt taaaggtcct
     1081 tttgttgcgc cctatgccac aaaactcggc tggctaaatt tcctgatacg acttgaaccc
     1141 gaatatttca aaggtatacc acgaacgaaa attattgacg cattaagggc agaaggcata
     1201 gaagcagcaa caggaagacc tggatatatt cctcttgtga gacagccgct actaaatgaa
     1261 atagaacacc atcctgcata tgtcatctgg taccactttt tgaacaatca tcctgaatac
     1321 aaaattgacg attatcctgt ggctgaaaag gttctttttg aaaggattgc aataccatcc
     1381 atgagggaca ttgaatatga tcaggagtat ctccaacaga tttgtgatgc cttcaggaaa
     1441 atatcgcaac ataaggacga actgaaatga tggaaatcat tgactgccat attcatccag
     1501 cagttgacag gcaaacggat ttctgcttct atggaagtcc tggtagtatc aaaaaacagt
     1561 ttgaagacct gaaaagggca ggtatctcaa aggcatgtgg aagtgtgatt gttgcgaaaa
     1621 ctccatctgt ttttgatgag gtcaaaaaac tcaatgattt tgctctctcc atcagggaca
     1681 gatatcctga tttttatatt cctggtattc aaatacaccc cagttttcca gatgaatcat
     1741 gcagggaaat cgaacgctgt tgcacacagc accaggtaac atggattgga gaacttgtcg
     1801 gttatcttat gggttttcct gatgattatg catcaaaaag tatgataaaa atcatgaaat
     1861 ttgcttcaca atacaaagtg gtcgtgaatt tccattgtgg cgatatgaaa attgtggaaa
     1921 aactgtgtaa aagtgtgcct gaaatgaatt ttgttcttgc gcacccaggc gaaagggaac
     1981 attttctcag ccgtattaac aaggtggcag aattaaaaaa cctgtatctt gatatttcag
     2041 gtacaggcat tgacagatac agaatgctaa aaaaggcagt ggacattgca ggttataaaa
     2101 aacttttgtt tgggtcagat taccctataa ataaccctgc tgtgtatatt catggtgtgc
     2161 tatttgaaaa actttcagaa aaagccacac aatcaatgtt cgcagataat tttacccgtc
     2221 ttataaaaag gggctgaaca tgttcaggaa attgtgtgtt ttgattttgc ttgttgttgc
     2281 cactggttat tgcagtgagt tttcacatct tgaaccactt ggaatccaat acagttatca
     2341 ttatcttgat acgccaaggc ccatcagaat acatgtactg aaaatagacc tgacaaaaaa
     2401 taaggtagtt ccattagtta ttgtggcaaa agaccctgat ggaaaaggtc ctgcagaagc
     2461 cgtattaaca aatccttttg cccttgtcgg ttgcatggat gatattgttg cctttgtcaa
     2521 cacaaatccc tgggatagtt ttccagatga aaaaggagaa aaaaatactc actggtatga
     2581 gggtcaacac gtgaatattt ttgggctggt tgcaactggt ggcaaaatca tcagtcctgc
     2641 tggagacggc tgtatttctg tctggtcaga tacttctggg aaaatcaata tttcaaaatt
     2701 tcctgataca gatacccttg ttgaaggcgt tgcaggatgg tatcagattg taaaagacaa
     2761 aacaattatt ccaaaacaga cgaatcaact caaccctctt acaggcatcg gcacagatgc
     2821 cacagggtat atcatgtggc ttgttgttgc tgatggaagg caaaaagggt tcagcgaagg
     2881 aatgagccac tatgagcttg ctgaatttat gttaaaactt gggtgtgaac aggttgcatt
     2941 gatggatggc ggtggcagca gtatcatggc aatcagggac aaaaatggaa gatggcaggt
     3001 agtaaacagt ccttcagaca gaatcctttt tatcaagaaa atcagaccag tgcctgtgat
     3061 tctgggaata aagaaaaaat aaattagtgt tctatcggac tatctgcaat ttccttatga
     3121 tactcttgaa gagattttac tgaataacta ctgccagatt ttatcattgc ctcaattcct
     3181 tttgctgctg catgcgctgc tgcaatagtt gtcatatatg gaattctgta ttttatggca
     3241 tttttcctga tataggaatc atcatattga ctttgttttc ctgctggggt gttaataatg
     3301 acatgaattt gaccgttttt aatcatgtcc acgatgttgg gccgctgctc gtgaaccttc
     3361 catgcaatgt cacatggtat accatgattt ttcaaaatgt ttgctgttcc tgttgttgca
     3421 tagattttga agccaagtgc atgaaacttt tttgcaacag gcacaatgaa ttgcttttcc
     3481 ctgtctgcaa ctgtaattaa cactgcccct tgtaatggca ggcgctgacc tgctgcttct
     3541 tctgccttat aaaatgcagt gccaaaagtg tcagccatgc ccagtacttc tccagtagaa
     3601 cgcatctctg gacccaggag tgtgtcaacc tcaggaaaca tattaaaagg aaacactgct
     3661 tctttgactc caaaatggct cagttttctc ctgacaacat tcaatgaatt taattgttct
     3721 cctacgagaa gttttgtggc aatttttgcc ataggaatat tacaaacctt tgaaacaagg
     3781 ggtactgttc ttgaagcgcg cgggtttgct tcaagaatat acactttgtc gtcacatatc
     3841 gcgtactgaa cattgatcag gccaacaact ttcaatgttt tcgcaattgt ttcggtgtag
     3901 tgataaattg tttcaaggtg tttcgctgga atgcctgtag gtggaatcac gcacgcagaa
     3961 tcaccagaat gaatccctgc ttgttcaatg tgttccatca cagccgggat gatgcaatcg
     4021 gttccatctg aaatagcatc tgtttctgct tcaaccgcat tttcaaggaa tttatctatc
     4081 agaatgggtc gttcaggggt gatgcctact gcagcagata catattcccg caacatttgt
     4141 tcgtcatata caacctccat gccccttcca ccaagcacat atgatggtcg taccattaaa
     4201 ggatacccaa tttgttctgc aattttcact gcctctttct cactggatgc catacctgaa
     4261 gcaggcattg gaataccatt ggtttccatc attttcctga atctatccct gtcttcagca
     4321 aggtctattg tgtcaacagt tgttccaagg attcttactc cttcagattc aagttgcctt
     4381 gcgatattca ggggtgtttg gccaccaaac tgcactatca cgccttctgg tttttctttc
     4441 tgatagatac tgacaacgtc ttctactgta aggggttcaa agtacaatct atctgatgta
     4501 tcataatcag tggacactgt ttcagggttg cagttaatca taatggtttc atatcccatt
     4561 tctttcaggg caaatgctgc atgaacgcaa cagtaatcaa actctatgcc ctggccaatt
     4621 ctattgggtc caccgccaag aataatcacc ttttttcttt ttgatacttt tattttatct
     4681 ggtccattgt acgttgaata aaagtatgaa gcatcctcca ctccgcagac aggtactgcg
     4741 tcccaggatt gaacaatctt aagttgtgtt cttctgtttc gaatgtcctg ttctgatacg
     4801 cctaaaatct ctgccagata cttatcagaa aatccatctt tctttgcctg ggtcagcagt
     4861 ttatccggaa ttgcttttcc tctgtgtttc aaaatctctt cttcaagttc aacaagttcc
     4921 ttcatctgct ggataaacca tggcttgata tatgtttttt cgtacaactg gtcaatggtg
     4981 gctccttttc ttagcgcttc gtacataaca aactgtcttt cgctggtcgg ataagaaagc
     5041 atttcaagaa gttcctgcaa ggacttctta ttgaaatctt ttacaaaacc aaaaccataa
     5101 cggcctgttt caaggcttct tattgccttt tgaagtgctt ctttatatgt ttttccgatg
     5161 ctcattacct ctccaactgc cctcatctgt gttccaagtt cgtctttgac gcccttaaat
     5221 ttttcaaatg cccatcgtgc aaacttcaca accacatagt cccctgatgg cttatacttt
     5281 tccagggttc cttctttcca gtatggaatt tcatcaaggg taagccctgc tgcaagtttt
     5341 gcagatatca aggcaatagg aaaaccggtt gcctttgaag caagcgcaga tgaccttgat
     5401 gttcgtgggt tgatttctat gacaacaacc ctgtctgttt ttgggtcatg cgcaaattga
     5461 atattggttc ctcctattac tttgattgcc tcaacaattc tgaaagaata ttcctgcaat
     5521 ctctgttgca atttttcatc tatggtaagc atgggcgcaa cacaaaaaga atctcctgta
     5581 tgaacaccca tagggtcaac attttcaata aaacatactg taatcatctg gttttttgaa
     5641 tccctgacaa cctccagttc aagttcttcc catcctatta cgctttcttc aataagaatc
     5701 tgtcccacaa ggcttgcagc aagacccctt gctgcaacaa ctttcaattc atccctgtta
     5761 tacacaagcc caccacctgt accacccagg gtgtaagcag gtctaattac cacaggataa
     5821 ccgagcctat ctgcaatttt ttctgcttct tctatagaat atgcaggatc actttctggc
     5881 atcggtatcc caagcatttt catggtctct ttgaaaacaa ttctgtcctc tcctctttca
     5941 atggcgtctg cttcaactcc aataattttt acgccgaatt ttttaaggat gcctgtttta
     6001 tgaagcacag aactgatatt cagccctgtc tgtccgccaa ggtttggcag aagagcatct
     6061 ggtttttcct tctcaataat cttctctatt gcctcgggtg tcagtggttc aatgtaggtc
     6121 acatctgcca tttcagggtc tgtcatgata gtggctggat ttgaatttac aaggataatt
     6181 ctataaccaa gatgtctgag tgccttacat gcctgtgttc cagaatagtc aaactcgcat
     6241 gcctgtccaa taacaatggg tcctgagcca ataattaaca cacttttgat gtcgtgtcgt
     6301 ggcggcattt ttctcctttc cttgttttta atatactatt gcaattattt gattaaatat
     6361 caacagtttg ttcgccctta agaagggctg tcagctgcga ccattttgcg gcgatcaatc
     6421 tgtatatgcc tgcatacttc cttttcatct catccacagg gatgtcttct gttcctgttt
     6481 ctgtcgttct cctgaaacag aaacccagca tgtcattgaa aacaaaccac ttttcagaat
     6541 ccaccaccat acacaaccct ggatgattga ctccctccct tccttttacg actgcaagca
     6601 taaatcgaac atttgccata gcacctccta tttgcaaaga ccggtatacc ggataactgt
     6661 tgccatcgcc tcaacgccaa tggcaagaca gcgttcatca aggttaaact tgttactatg
     6721 atgaaaaaat ggacttttcc cacaaccaat attgatatag caggatggta ttttctggct
     6781 gtaaaaagaa aaatcttcgc tgcccatcgt gggtttttcg tctattttta ctgctctgtt
     6841 tccaaaacag gaagaaagta ttgtaataac cttttcactg agggacgggt tgttgtatgt
     6901 cgcaggaaca aactcttcat agtgtatctc tgtctttact ccaaacccat cttccagccc
     6961 tttcaacatt ctcttcatgc tttttttcgc cagcatattc tggtttttat cgtatgtcct
     7021 gacagttcca agcagttcaa cctgttcagg gataatgtta aaggcatcac ctgctgtaat
     7081 tttgcccaca gaaatcacaa gcggattctg cggatctgat tgcctgctta caatcgtttg
     7141 taattgcgta ataaagaatg ctgcagcaac aacagggtct atggctttat gcggacttga
     7201 gccatgtcca cccttacctt tgatgaaaat tgtgaatcta tctgcggatg ccatgacagg
     7261 acccttattt actgaaaatt ctcctgtatc aagccccgcc cagacatgaa atcccagtaa
     7321 gtgcttcatc ccatcaagcg caccttcttt aatcatccct ggcgcaccac atggcatgga
     7381 ttcttctgat ggctggaata agaatcttat tgaaaacggt atggacttta tctgtgaaaa
     7441 aatctttgca acaccgagtg caatcgccat gtgagcatcg tgtccgcatg cgtgcattac
     7501 accaggatac acagaagaaa actccaatcc tgtctcttcc tgaaccggta aggcatccat
     7561 gtctgccctc aatccaatta agcgattgcc tgttcctgca atttccgcaa taatccctgt
     7621 accagcagcc ctccgataag gtatccccat cttttccaga agattctgaa taaagtgtga
     7681 tgtttgaact tccttgtttg aaagttctgg aaatttgtga aaatgccttc tccaggcgat
     7741 aacatcctct tctattgata ctaccagttt gcggaaatca atttcagtca tggtcttttc
     7801 aaaaaagctc taatccaggg atatttcggt cataccgccc atatatggtc tcagaacttc
     7861 tggaatgatg atagttccct tttcagtttg gtagttttcc attatggcaa taatggttct
     7921 tggtaacgca atccctgacc catttaacgt gtgcacataa tctgtttttc ctgatgccga
     7981 acgataacgt atgtttgccc tgcgcgcctg gaatgcctca aaatttgagc acgatgaaac
     8041 ctccagccac ttcttcaccc ctggagacca gacctcaagg tcataacatt tggctgctgc
     8101 aaaactggta tctccagtac agagaagtac caccctgtaa gcaagtccaa gcatttgaag
     8161 cactttttct gcttcctgaa caagttgctc atgttcatcg tatgagtgtt gcggatgcga
     8221 aaatatcaca agttcaacct tatcaaactg atgaagtcgt attaaacctc ttgtgtcgct
     8281 accatacgcg cctgcctctc ttctgaagca tggactgtag gcacaatacc tgatgggtaa
     8341 tgtatcctct ggaattgttt cttctctatg ataatttgtt acaggaacct ctgctgttgg
     8401 tatcaaaaac aggtcctctt ccttcaatct atacatgtcg tcttccagac gtggtagctg
     8461 ccctgttcca aacatgctgg ctcggttgac cagggcaggt ggccagagtt caacaaaacc
     8521 attttttcta tgaaggtcca gcataaagtt tatcaacgcc cttacaagga gcgctccttg
     8581 accagaaaac gcaggaaaaa aactacctgt catttttgct gccctttcca gctgcaacac
     8641 tcccaggttt ctacctattt catagtgcgg aatgatttca aacgagggct gtttaatttc
     8701 gccccaggtt cttacaacaa cattatcgtt tgatgttctg ccagcaggga cactcgggtg
     8761 aggtatattt ggaatactgg caagcgtcgt atctatttgc gcctctattt cttttaaaca
     8821 atcttctttc tcttttattt ctgaagaaat ctgttttgcc tggagtagaa gatggtttgc
     8881 atcctgcccg ttttttttca tctgggcaag gtctgtactg agttttttgt gcaaccgttt
     8941 tttttcttca agatcctgaa tcatctgtct tctttgctgc tcaaggttct gaaacgatgc
     9001 ccagtcaaca gtaatattct tcaattctgc tgctttcttg ataagatcca ggttttcgcg
     9061 gacaaactta atgggaaaca ctttcgcctt cctttatctg aactttatac tctcctggtt
     9121 tcatcatgcc aagttgttcc ctggctattt tttctgtcat gaaaggtgtt tctgcctgct
     9181 tgagagtttt ttcaagcatg gcattctcct gggttaattt ttttatctcc tgctggtaca
     9241 ttaacagttt gctcttctgt acatagtact gttgcagggc atacaaaatt acaatggttc
     9301 ctgaaacgag gatcattatt ttctgcaggg aaaagaaccg atattttttt gccatgtttt
     9361 attataaagt ataaaaaaat gaaggcgtca tgtcaaattt caggtttcac caccagcact
     9421 ccttctttct tcaatgatag caaatgcctg tggacaaaag aataaacata acccctggac
     9481 tcaatgtgaa caattttgta tcctgcttgt tttaactgcc tcaatgcggt tttcgtcttt
     9541 gagataccaa tctgtggaaa cctgtgatga aattcaacaa caacttgtcc tggcctgatg
     9601 tcggattcta ccatatcagc aatcacagca tattctccgc cttctatgtc aagttttaac
     9661 aaatcaatac gtacatgtcc aagttctttc ataatggtcg ccagccgcat gacacacact
     9721 tcaaatggtt caacagaagt ttttcgggct aaaggaaacg aggtactatt ggggttctct
     9781 ggcggaaaca tcaacatttc tccatcatgg tcagatattc cataagggaa aaacctgaat
     9841 tccagaggaa ggttgtattt ttcaatccag gcaatactga cgggtgatgg gtcaaaacca
     9901 aaaacatgcg cgccaaaatt ctttattata tcaaggtcaa aactgatgtc atctccgatg
     9961 cccgctgaat aaacaatact gcgggatgta agaaaatcag ggcatatcac atattgttct
    10021 tttcctgctg tgatggtctg gattttaagt tgcctccacg tccacacatc ctttccgcac
    10081 atctgtttca caaacctctc tgcccttctt cttccatcag taaaggtcat tacttataac
    10141 cctctgtggt tatatgttcc acaaaccatt ttccggtaag gcccaatatc tgacttttgt
    10201 gttgtagatt ctgccttctg cagggaatca tgtccaccac aacaaaacca caccctgtaa
    10261 gaattctctc aagaaaaaag cagcacatta ctgcgccacc tccaccgccg ccggccacgc
    10321 atattccgat tgcaggcttt ccttctattc cggtcttgcc tgcctgatgt cttgtgattc
    10381 ttctcaatct atcaagaaaa ctcttcatgc tttctgagat gtcgctaaag tacaccgggg
    10441 tcgcaaaaac aatagcatct gcctgcctga ttttttcaac aattgagtga aaatcatcct
    10501 ctatggtgca tattccttct gtccggcatt ttccccatcc attgaaatca cactgtatgc
    10561 accgttctat cttttttgcc gttaaaaaga tttgctcgca ctgatgatct ttgccagccc
    10621 catcaaacaa tgctttcacg cacagacctg ttttaccttc tggatttctg cttcctgtaa
    10681 gagcaagaat gttcatgttt cacctcccca atagcttttc agagtggatt aagtcaatct
    10741 atttatatta taatacaata ctaacaacat cagcaacctg gctcttgact ttttattgaa
    10801 atcatgagaa actgaatctg gttaaaattt atcaaatctc gtccataata agccggggta
    10861 gctcagtggt agagcgcagc cctgaaaagg ctggcgtggg cagttcgatt ctgccccccg
    10921 gcatacaaga tggttgctac aatggattat tatcagaatt tcctgaaaag gttgactgtt
    10981 ctcaatcccg ggaccaggtt attgatcaac aggattctca acaggaataa ggttcagttt
    11041 atgaacgaac ttgttgaaat cctgaaaaag gtcaatgccc ttgaatcaga aatacagaag
    11101 tatcccctgg accagttgag agcaaaaacc ggctacttca aagaaagact ttcaaaaggc
    11161 gaatcccttg attccattct gccagaggca tttgctgtcg tcagggaaac tgccagacgc
    11221 gtcataaata tgcgacattt tgactgccag attgtcggtg gtgttgtact gcacagggga
    11281 aatgttgcag aaatggcaac aggcgaagga aaaacccttg ttgccacgct tcctctgtac
    11341 ctgaatgctt tgacaggcag gggatgtcat cttgtcacag tcaacgatta tcttgcaaaa
    11401 agggacaggt tctggatggg tcctgtgttt gaagctcttg gattgagtgt tggtctcatc
    11461 cagcatgaca tggttcctca cgaacggcaa aaagcgtatc atgcagacat cacatatggc
    11521 accaataatg aatttggctt tgactatctc agggataaca tggcatggcg gttagaagac
    11581 caggttcaac gaccacttta ttatgccatt gttgatgaag ttgacagcat attgattgat
    11641 gaagcaagaa cacctttgat tatttccggt ccttccgaag aatcagtttc tctatattac
    11701 gatatcgaca gggtcgtaag aagattgaaa aaaggcgagg actatactgt tgacgaggaa
    11761 agtcaaactg caagtttaac agaaaccggc gtagcaaaat gtgaaaaaat gctcaatgtg
    11821 acaaacttat acgatatttc aaacacagag attgtccacc acatcataca gtccctacga
    11881 gcacataact tttatcacag agacgaccag tacattgtca aagaaggcaa ggtaacaatt
    11941 gtggatgagt ttacaggtcg tctgatgcca ggtagacgat ggtcagacgg tctccaccag
    12001 gcagttgagg caaaagaagg cgtgcagatt gaaagcgaaa atcagaccct tgcaacgatt
    12061 tcttttcaaa actatttccg cttgtatgaa aaaattgctg gtatgacagg tacagc
//
LOCUS       DAORYU010000015        12108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_4070_length_12108_cov_5.060815, whole genome shotgun sequence.
ACCESSION   DAORYU010000015 DAORYU010000000
VERSION     DAORYU010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12108
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4070_length_12108_cov_5.060815"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /locus_tag="PLW07_01035"
     CDS             complement(<1..1098)
                     /locus_tag="PLW07_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49140.1"
                     /translation="MRKLLVLIMLFVCIFSFATHAQQKKNPEKLYVRFKVLSADQVYA
                     YLKTIIHHSPWQKNYPTVPRGGENYARLRIKQNNFSPWAEIDGDWGTIVFDFRNPQPL
                     ENVEVEIQLSIKDDESGIFKTMKIQEKGSIVAISLPPDYYENPEGILTVRQTSEKHLK
                     MAQSLNLSQETLPKKFSFCTGASGYDSLYKDAEVWKNELKTLKILGINGTTYPGNAEQ
                     YSVYKELGFDRFITYNPGNPEQAKREKEISEEAFSKIQTVVIADEPGNHGLYQIKNEP
                     VENFHKFLESKGLKPKDFNAKNWSDIQPITDKNEIAKIEYNWGPKYGEAARKAYYWTI
                     RYTEHLTNQYFKNITDLLEKQYLPGTLTFVNY"
     gene            complement(1132..2436)
                     /locus_tag="PLW07_01040"
     CDS             complement(1132..2436)
                     /locus_tag="PLW07_01040"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOL49141.1"
                     /translation="MLKKCLYGCLVFIIFVALSGFAQVVCTPDRNTVSIKSGDIFVFT
                     VSNPNPPQDISIKVYDANGKFVRTLFEKCTTETQFKIPWNLKDTDGKTVSPGKYTVKI
                     QIGLNLVLETSFGKNGILGGDDTFISPRNVRVDKKGCVYVLDYGAGIIFKFNPDSTPA
                     NDIAGKNKITGPSSPYWVNIAVDEDGRIYGSVGHSVLVYDGKTGEKIYHIGEFFGNDV
                     GWETTKGGLGWPNWVGLNADYRLYASCPGYSYWAAFDRRKTGKEGGLWRSKGGIPGES
                     GDTDGKKTIYLAHPYYTNRNGITKWLDKDTSVDITYSITSFTDPVKKTTENMANVYGV
                     AFDKESGIFLVQRDPVRIIKISDNGFGFDFVKQFASPGNDASKLEFMAPKDAAVSPDG
                     KYLYVIEDGEPISKTNTAPGLARLVKYKITYQDEKQITINVQ"
     gene            complement(2480..3928)
                     /locus_tag="PLW07_01045"
     CDS             complement(2480..3928)
                     /locus_tag="PLW07_01045"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOL49142.1"
                     /translation="MADWKSEITKIAPCKWMIPRKNQMRVPGIIYISEAMLHRTFEDN
                     VLQQVANVGCLPGIISHSLAMPDVHWGYGAPIGGVAAFRKDSGVIVPGFVGYDINCGV
                     RLIRSDVMVSEIKDKLEKIVYCLYSNIPSGVGSTGKIVLKTKQVDEVLSQGASYALKQ
                     GYGWNSDIERTEETGCMKTADPECVSPRAKERGADQLGTLGSGNHFLEIQQVAEIYNR
                     EAANVFGLFENQITVMVHTGSRGLGYQVCDDYLATFGRVLSKYGITLPDRQLACAPVD
                     SKEGKDYFAAMSAAANFAWANRQIITHWIRECFEKVFSIPAERLGLHVVYDVAHNIAK
                     YEKHSFEDKNESVYVHRKGATRAFPKGHQAVCEIYRNIGQPVIIPGTMGTSSYVLIGT
                     EKALNETWGSTCHGAGRVMSRAQAIKKSKGRKIEQELLAAGVIAKAKSRGGLDEEIPE
                     AYKDVDEIVRVVEEAGLSTKVAKMKPLAVIKG"
     gene            complement(3921..4496)
                     /locus_tag="PLW07_01050"
     CDS             complement(3921..4496)
                     /locus_tag="PLW07_01050"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOL49143.1"
                     /translation="MKNKNTRLWKIILGGSGGQGIVTLGKILAYAGVMKKLNVSFLPA
                     YGAEMRGGYVYSMLTLSTGELVSPVISGAGYGVFLNENSMQLLGQYLKEDAWIVWNSS
                     LIKSARQSRNNDIGIPASEIAEKLGSSKIANMVMLGGLLKILEKSSFPVTEKEIAKSI
                     KVFLTESKNSTLNFKAVSEGMKLVETVLKHG"
     gene            complement(4493..5458)
                     /locus_tag="PLW07_01055"
     CDS             complement(4493..5458)
                     /locus_tag="PLW07_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020279282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOL49144.1"
                     /translation="MKLGVFLVLFGGKKFEEALDIAKDLGLETVEIGTGNYPGNAHCN
                     PEELLADDSKLKSFSKAVGKRGLDISALSCHGNPLHPDRKFADAHRHAQRQTILLAEK
                     LGIKRIIGFSGCPGDGENAQSPNWVTCPWPEDFSKILAWQWEKCVIPYWKEEVEFAKK
                     HSVTQICLEMHPGFVVYNPETLLKLRNAVGEVIGANFDPSHLFWQGIDPIAAVRKLAG
                     AIYHVHAKDTKIDGINCSVNGVLDTKTYLDEINRSWIFRTVGYGHGEEFWRDFVSNLR
                     LVGYDGALSIEHEDSLMSIDEGLKKAISFLKNILISEPKPKAWWT"
     gene            5541..6722
                     /locus_tag="PLW07_01060"
     CDS             5541..6722
                     /locus_tag="PLW07_01060"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOL49145.1"
                     /translation="MNRCLFFLARRFIGRRHKESFIYFTSFVSVIGIALGVASLMIVL
                     GVMNGFSNDLKRKIIGAQPHIVVEGNPYLTDYKKIAEHLKMMPGITGTGYFAQTQIIY
                     KSSQYLIGGYLRGIEGDSEIACVKKFIKQGNLEGIYSDGIILGSELAKELDINVNDTV
                     VIIGGIPPREYSFKVAGIVEYGVYNVDVSTGIISLENFQKKFFSGRQMASIGLTVNDI
                     YHCEKIAEKVRNIVPADAQVSTWIKKNKILFAALALEKKAMLLILAIIIIVASFNIAS
                     SLMMTVYRKIREIGVLKAIGMTKSQIRAVFLIQGFLLGIRGLVYGLVLGGIIIWVLKR
                     YNIVHLPEYIYSISYLPVEISGIEIFSICAAVFITTLVASIFPAYRAGKLEPAAALRY
                     E"
     gene            6719..7411
                     /locus_tag="PLW07_01065"
     CDS             6719..7411
                     /locus_tag="PLW07_01065"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOL49146.1"
                     /translation="MNIAENYAIICAENIVKSYETNVKALDDITLAIPKGNITVILGP
                     SGSGKTTLLNILALLDKPNSGKVILDGRDVSSLEERDAAMIRNRMFGFVFQFFHLIPE
                     LNVEENIMIPLMIRQPNSSLNEHREKVLKVIKEFHLENKLHKYPNNLSGGEKQRIGIC
                     RSLICDPEVVFADEPTGNLDRESSFELISLIKFLNEEKGKTFVVATHNENFLKIATNV
                     IYLSNGKITNRE"
     gene            7414..8286
                     /gene="ilvE"
                     /locus_tag="PLW07_01070"
     CDS             7414..8286
                     /gene="ilvE"
                     /locus_tag="PLW07_01070"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HOL49147.1"
                     /translation="MGLWIYLDGKFVDEKHAKISVFDHGLLYGDGVFEGLRSYDGKIF
                     RLSQHIKRLYKSAKAILLEIPVSMEEMAEIIKKTIKKNKLKDSYIRVVVTRGVGDLGL
                     DPRKCPEPTIFVIASKIKLYPEEYYEKGLSVVTIATRRNLTESLNPAIKSLNYLNNIL
                     AKIEATNAGAMEGLFLNNEGYVAECTGENIFIVVENKLFTPPIFAGALDGISRDAVIQ
                     IGREIGLDVEEKLLTRYDLYNSDECFLTGTAAEIIPVTLIDGRTIGSGKLGPLTRRIR
                     EEFHRLVQKDGTPI"
     gene            8335..8745
                     /locus_tag="PLW07_01075"
     CDS             8335..8745
                     /locus_tag="PLW07_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49148.1"
                     /translation="MEISAGGKATTGCFCFDCVRNNPEIKTAFETFKIRHKTISYKQK
                     NCPFCGMTLEDWKQSGFAGCATCYQIFRTHIKREIKKCQHGSLHRGKFPHSENVSKNF
                     LVYFENEMRRAVAGRDIEKVKKIKQEIEMFIKNG"
     gene            8738..9775
                     /locus_tag="PLW07_01080"
     CDS             8738..9775
                     /locus_tag="PLW07_01080"
                     /inference="COORDINATES: protein motif:HMM:NF012443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49149.1"
                     /translation="MDKYLRDLVFNEPLLSDEPTFFTARLRLARNIKQYPFPHQMSEK
                     QAEQLTREVIDVSTSITDEAPVIISMSDIDNVTRDVLIERHIISPEMAEYGFGKILIW
                     FPEMKLRIFVNEEDHFRISICGGKEDLCNLWNVLNHVDDILSRCFDYAFDKEFGYLTC
                     CPTNVGPALRVSSLFFLPALKMTGKIKLVFDTISRIGCIIRGFYGEGSRSIANVYQIA
                     TGPALGKQEIELCQDFESVSRTIKKQELMSMNIIDHMAIKGKIVCFLNRIFENNRGIN
                     SSNSIKGLSLLLFGRNLGIIHMNVVTLKTLFYRILPASLQLEAGTEMDAMERDNLRLQ
                     ILRNELRSIYV"
     gene            9768..>12108
                     /locus_tag="PLW07_01085"
     CDS             9768..>12108
                     /locus_tag="PLW07_01085"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOL49150.1"
                     /translation="MFEQYFERFTKRVKTVLELARQHAIRYGHAQIETEDVLLGLIDE
                     GQGMGVAVLHFLKIDPEHIRFELEKSIRVGIPLLSSAEIPLSPGVKRVLALSYRQAQY
                     FGHSYVGTEHLLIGLVAEEEGHAARVLVRFGLTVDSIRKAIEGLLWGEPENVSKGKQH
                     SSQHTSQPQTKSKTPTIDSYGKDLTQLAEGKKLDPVIGREKEIERVTQILCRRKKNNP
                     VLLGEAGVGKTAIVEGFAQRIASGNIPEILKHKRIIMIDLASLVAGTKYRGEFEQRVK
                     LLLDEIKQNKNIIIFIDELHTLVGAGGAEGAIDASNILKPALSRGEIQCIGATTFDEY
                     RKYIEKDAALERRFQPVVINPPTVQETIEILWGLRPKYEEHHKLKIIDEALVLAAKLA
                     DRYITGRYLPDKAIDLVDEAASKQRLLFSNTPVELEELEKRAAQLVNLQEQATIQQDF
                     EKAARIRDRLREIRSRINEVKLSWEKAQPDTHRIVTGKDIAEIVSKWTGIPLSQLCKE
                     EREKLLEMEEHLHRVVVGQDEAISAVSRAIRRSRAGVKDQKRPIGSFLFLGPTGVGKT
                     LLARALAEFLFGDDQALIQIDMSEYMEKFAISRLIGAPPGYVGYEEGGQLTEKVRRRP
                     YSVVLLDEIEKAHPDVFNLLLQVLEEGRLTDSFGRSVSFQNGILILTSNIGTQHLKNR
                     SAIGFQENKEEIPYSAVKARILDEIKKTFRPEFLNRLDEIIVFHPLEQEHLLKIVDLE
                     VGKVAERLKEQGFTISLTSEAREFLKNMGTDPQFGARPLR"
BASE COUNT         3616 a         2292 c         2523 g         3677 t
ORIGIN      
        1 atagtttaca aatgtgaggg ttcctggcag atactgtttt tcaagtaagt cagtaatatt
       61 tttgaaatac tgatttgtca gatgttcagt gtacctaatt gtccagtaat atgcctttct
      121 tgcggcttct ccatattttg gaccccagtt gtattcaatt tttgcaattt catttttatc
      181 tgtgatgggt tgaatatctg accagttctt tgcattaaaa tcctttggtt tcagtccctt
      241 actttcaagg aatttatgaa agttttctac tggttcattt ttaatctggt atagcccgtg
      301 atttcctggt tcatcggcaa tgacaactgt ctgaattttt gagaatgcct cctctgaaat
      361 ttctttttcc ctttttgcct gttcgggatt tcctggattg tacgtgatga atctatcaaa
      421 tccaagttct ttatagacac tgtattgttc ggcattacca ggataagttg ttccattgat
      481 acccaggatt tttaatgttt tcagttcatt tttccagact tcagcatctt tgtacaaact
      541 gtcatatcca ctggcacctg tgcaaaaaga aaattttttt ggcagtgttt cctgagaaag
      601 attgagggat tgagccattt tcagatgttt ttctgatgtc tgtcttactg tgagtattcc
      661 ttctggattt tcgtagtaat caggtggtaa actgattgca acaatacttc ctttttcctg
      721 aattttcatt gttttaaaaa ttcctgactc gtcatccttg attgataatt gaatttcaac
      781 ttcaacattt tccaatggct ggggatttct gaaatcaaac acaattgttc cccaatcgcc
      841 atcaatttct gcccatggtg agaaattatt ttgtttaatg cgcaaccttg cgtaattttc
      901 gccaccgcgt ggaactgtgg gatagttttt ttgccatgga gaatgatgaa tgatagtttt
      961 caaataagcg tatacctgat ctgctgaaag taccttgaat cttacataga gtttttcagg
     1021 atttttcttc tgctgggcgt gtgtagcaaa agaaaaaata cagacaaaca gcataatcaa
     1081 aaccagcaat tttctcatag cgacccttta tttgaagtta aagtcgaaaa tttactgcac
     1141 gtttatcgtg atttgttttt cgtcctgata agtaatctta tactttacaa gtcgtgcaag
     1201 ccctggagca gtgtttgttt tgctgattgg ttctccatct tctattacat aaagatattt
     1261 tccatcagga ctgactgctg cgtcttttgg tgccatgaat tcaagtttag acgcatcatt
     1321 tcctggagag gcaaattgtt taacaaagtc aaacccgaaa ccattatctg aaattttgat
     1381 aattctcaca gggtctctct gaacaaggaa aattcctgat tctttgtcaa atgcaacgcc
     1441 ataaacattt gccatatttt cagtggtctt ttttactgga tcagtgaaag acgtaatgct
     1501 atatgtaatg tcaacagaag tgtctttatc cagccacttt gtaataccat tgcgattggt
     1561 gtaataggga tgcgcaagat aaatggtttt cttgccatct gtatcccctg attctcctgg
     1621 aataccacct tttgaacgcc acagtcctcc ttcttttcct gttttcctcc tatcaaaggc
     1681 agcccagtaa gaatatccag gacacgatgc ataaagacga taatcagcat tgagtcctac
     1741 ccagtttggc cagccaagcc cgccttttgt tgtttcccag cccacatcat ttccaaaaaa
     1801 ttctccaatg tgatagattt tttctcctgt cttaccatca tacacaagca cactgtgacc
     1861 gacactgccg taaattcttc catcttcatc cactgcgata ttgacccagt aaggtgacga
     1921 tggtcccgtt attttatttt ttcctgcaat gtcattggca ggtgtgctgt ctggattaaa
     1981 tttgaatata atgcctgcgc cataatccag cacataaaca caaccttttt tatcaaccct
     2041 gacatttctc ggactgatga atgtatcatc tccaccaaga ataccatttt ttccgaaaga
     2101 tgtttcaagg acaagattca accctatctg aatttttaca gtgtatttcc ctgggctaac
     2161 tgtttttccg tcagtatcct ttaaattcca tggaattttg aactgtgttt cagtggtaca
     2221 tttttcaaaa agtgtcctca caaattttcc atttgcatcg taaaccttga tggaaatatc
     2281 ctgtggtgga ttcgggttgg aaactgtgaa gacaaaaatg tctcctgatt ttattgaaac
     2341 cgtatttcta tcaggagtac aaacaacctg agcaaaacca gacagagcca caaaaattat
     2401 aaaaaccaga catccataca aacatttttt taacattttt tcactcctgt ttttttgtaa
     2461 atgaaattca cttacctttt tatcccttta tcactgcaag gggtttcatt tttgcaactt
     2521 ttgttgatag acctgcttcc tcaacaactc ttacaatttc gtcaacatcc ttatatgcct
     2581 caggaatttc ttcatcaagt ccgcctctgc tttttgcttt tgctataacg ccagcagcca
     2641 gaagttcctg ctctattttt cttcctttgc tttttttgat tgcctgggct cttgacatca
     2701 cccttcctgc tccatggcag gttgaacccc atgtttcatt caatgctttt tcggttccaa
     2761 ttagaacata ggaacttgtt cccatggttc cagggatgat aacaggttga ccgatgtttc
     2821 tatagatttc gcatactgcc tggtgtcctt ttggaaatgc tcttgtggca cccttacgat
     2881 gaacataaac agattcattt ttgtcttcaa atgaatgctt ttcatacttt gcaatattgt
     2941 gagcaacatc ataaacgaca tggagcccaa gtctttcagc aggtattgag aaaacctttt
     3001 caaaacattc ccgtatccag tgtgtaataa tctgtctgtt agcccaggca aaatttgcag
     3061 cagcactcat tgcagcaaaa taatctttcc cttcctttga atcaactggt gcgcaggcga
     3121 gctgtctgtc agggagggtg attccgtatt ttgacaatac cctgccaaat gtagcaaggt
     3181 aatcatcaca cacctgatac ccgagtcccc tgcttccagt atgtaccatc actgtgattt
     3241 gattttcaaa caaaccaaat acatttgctg cttccctatt ataaatttca gcaacctgct
     3301 ggatttccag aaaatggttg cccgagccaa gagtcccgag ctggtcagca cctctttctt
     3361 ttgctcgagg acttacacat tcagggtctg ctgttttcat gcagccggtt tcttctgttc
     3421 tttcgatatc agaattccag ccatagccct gtttcaaggc ataactggct ccctgagata
     3481 atacttcatc aacctgcttt gtttttaaaa caatttttcc tgttgaacca acaccagaag
     3541 gaatgtttga gtacagacaa tatacaattt tttcaagttt atctttgatt tctgatacca
     3601 tcacatcaga gcgaatcagt cggactccgc aattaatgtc atatccaaca aatccaggaa
     3661 caatgacacc actatctttt ctgaatgctg caaccccacc aatgggcgct ccatatcccc
     3721 agtgaacatc aggcattgct aaactatggg agataattcc aggaagacat ccaacattgg
     3781 cgacctgttg aagaacattg tcttcaaatg tgcgatgaag cattgcttca gagatataga
     3841 taataccagg tacccgcatc tggttttttc tgggtatcat ccatttacag ggcgcaatct
     3901 tagtaatttc tgatttccag tcagccatgt ttcagtaccg tttcaaccag tttcattcct
     3961 tctgacactg ccttgaaatt caacgttgaa tttttacttt cagtcagaaa taccttgatg
     4021 ctttttgcaa tctctttttc agtgacagga aatgatgatt tctcaagaat tttaagcaat
     4081 ccaccaagca tgaccatatt tgcaattttg ctacttccaa gtttttcggc aatttcagac
     4141 gcagggatgc ctatatcatt atttctggat tgtcttgcag atttgatgag ggaactgttc
     4201 catacaatcc aagcgtcttc cttaaggtat tgcccgagca actgcatgga attttcattg
     4261 agaaacacac cataaccagc ccctgaaatg acaggagaaa caagttctcc tgttgatagt
     4321 gttagcatgc tataaacata accgcccctc atttcagctc cataggcggg cagaaaactc
     4381 acattgagtt ttttcattac ccctgcatat gccagaatct tgcccagggt aacaatgccc
     4441 tgcccgccag agccgccgag gatgattttc cataagcgag tgtttttatt tttcaagtcc
     4501 accacgcttt tggttttggt tcgctgatca gaatgttttt aagaaaagaa atggcttttt
     4561 tcagaccctc gtcaatgctc attaaactat cctcatgctc aatgcttaat gctccgtcgt
     4621 aacctaccaa tctcaggttg ctgacaaaat ctctccagaa ttcctcacca tgtccgtacc
     4681 cgacggttct gaaaatccag gaacgattga tttcatcaag atatgttttc gtgtcaagga
     4741 ctccgttgac cgaacaattg ataccatcaa tctttgtgtc tttggcatga acatggtata
     4801 tagcaccagc aagttttctc acagcagcga tcgggtctat tccctgccag aacaaatggc
     4861 tggggtcaaa atttgcgcca ataacttcac ctacagcgtt tcgcaatttc aacaaagttt
     4921 caggattgta aacaacaaac cctggatgca tttcaagaca tatctgagta acactatgtt
     4981 tttttgcaaa ttccacttct tctttccagt aaggtatcac gcatttttcc cattgccagg
     5041 caagtatttt agaaaaatct tctggccagg ggcatgtcac ccagtttggt gactgtgcgt
     5101 tttcaccatc tccagggcaa ccagagaatc caataattct tttgatgccg agtttttcag
     5161 caagaaggat tgtttgtctc tgggcatgcc tgtgcgcatc ggcaaacttt ctgtccggat
     5221 gcagaggatt tccatgacag gacaaagcag aaatgtcaag tcctcttttt cccactgctt
     5281 tactgaatga tttcaactta ctgtcgtcag caagcagttc ttccgggtta caatgggcat
     5341 tgcccggata attacctgtg cctatttcta ctgtttcaag gcccaggtct tttgcgatgt
     5401 ccagcgcctc ttcaaacttt ttaccaccaa acagaaccag aaaaactccc agcttcataa
     5461 aggcctcctt ttttgtgata aaatattata ccctgcataa taatttattg ctatacagag
     5521 atttgtcaaa tattaaaact atgaacagat gtcttttttt tcttgctcgc aggtttatcg
     5581 gaagaaggca caaagaaagc tttatttatt tcacaagctt cgtcagtgtc atcggcattg
     5641 ctctgggagt tgcaagtttg atgattgtgc tgggagtgat gaacggcttc agcaatgatt
     5701 tgaaaagaaa aattatcgga gcacaacctc acattgttgt ggaaggcaac ccatatctga
     5761 ctgattacaa aaaaatcgca gaacacttaa aaatgatgcc aggaataaca ggtactggat
     5821 attttgcaca gacacagatt atttataagt cctcccagta tctgattgga ggatacctta
     5881 gaggaattga aggagatagc gaaattgcct gtgtgaagaa gtttatcaaa cagggtaatc
     5941 ttgaaggaat ttactcggat ggaattatac tgggaagtga acttgcaaag gaactggata
     6001 tcaatgttaa cgatacagtt gttattatag gtggaattcc gccgcgggaa tatagtttca
     6061 aggttgcagg tattgtggaa tatggtgtgt ataacgttga tgtgtcaaca ggaatcatat
     6121 ctcttgaaaa ttttcaaaaa aaattttttt caggaaggca gatggcaagt atcggtttga
     6181 ctgttaatga tatctatcac tgcgaaaaaa ttgcagaaaa ggtaagaaac atagtgcctg
     6241 ctgatgcaca ggtaagcaca tggattaaga aaaacaagat tctttttgca gcacttgctc
     6301 ttgaaaaaaa ggcgatgttg ttgattttag ccatcattat tattgtggct tcttttaata
     6361 tcgcaagcag tttaatgatg actgtgtata gaaaaatcag agaaattgga gtgctgaagg
     6421 cgatcgggat gacgaaatct cagattcgtg cggttttttt gattcaggga tttttgctcg
     6481 gaataagagg attggtatat ggactggtgc ttggaggaat tattatatgg gtgttgaaaa
     6541 gatataatat tgtccatctg ccagaatata tttatagcat ttcgtattta cctgtggaaa
     6601 tttcaggaat tgaaattttt tcaatatgtg cggctgtttt cattacaacg cttgttgctt
     6661 caatatttcc tgcttacaga gcaggaaaac ttgaaccagc agcagcattg aggtatgaat
     6721 gaatatagca gaaaattacg caatcatttg cgcagaaaat attgtcaaga gttatgaaac
     6781 caacgtgaaa gcccttgatg acattactct tgccatacca aaaggtaaca tcactgtgat
     6841 tcttggacct tctggttcag gaaaaaccac attactgaat atccttgctc tgctggataa
     6901 accgaattcg ggaaaagtaa ttcttgatgg cagagatgtt tcatcgcttg aagaaagaga
     6961 tgctgctatg atacggaata gaatgtttgg ttttgttttt caattttttc atctgatacc
     7021 ggaattgaat gttgaagaaa atattatgat acctttgatg atcagacaac ctaatagttc
     7081 attgaatgaa cacagggaaa aagtgttgaa agttattaag gaatttcatc tcgagaacaa
     7141 acttcacaaa tatcctaaca atctatcagg tggggaaaag caacgaattg gcatatgcag
     7201 aagtttgata tgcgaccctg aggtcgtttt tgcggatgaa cccacaggta atcttgacag
     7261 ggaatcttct tttgaactga tttctctgat aaaatttttg aatgaggaaa aaggaaaaac
     7321 ttttgttgta gccactcaca acgagaattt tttgaaaatt gcgacaaatg tcatatactt
     7381 atcaaatggg aaaattacaa acagggagta aaaatgggat tatggatata cctggacggg
     7441 aaatttgttg acgaaaaaca tgcaaaaatt tctgtatttg accatgggtt gctttatggt
     7501 gacggtgttt ttgagggatt gcgttcttat gatgggaaaa tttttcgttt gtcccagcac
     7561 atcaaaaggt tatataaatc tgcaaaagcg atactacttg aaattcctgt ctcgatggaa
     7621 gaaatggcag aaattattaa aaagacgata aagaaaaaca aactaaaaga ttcttacata
     7681 agggttgttg taacccgcgg ggttggagac cttgggcttg acccaagaaa atgtccagaa
     7741 ccaacaattt ttgtaattgc aagtaaaata aaactttatc ctgaagagta ttatgaaaaa
     7801 ggattatctg ttgtaaccat tgctaccaga agaaatttga cagaaagttt gaaccctgca
     7861 ataaagtcat taaattatct gaacaatatt cttgcgaaga ttgaagcaac aaacgcaggt
     7921 gcaatggaag gactttttct aaataatgag gggtatgtcg cagaatgtac aggagaaaat
     7981 attttcattg tggttgaaaa taaactgttc actccaccaa tttttgcagg ggctcttgac
     8041 ggaatttcaa gagatgctgt gattcaaatc ggtagagaaa taggacttga tgttgaggaa
     8101 aaattgctga caaggtatga cctttataat tcagatgagt gttttcttac cggtacggca
     8161 gcagaaatta ttcctgtaac gttgattgac ggaagaacaa ttggttcagg gaaattaggg
     8221 cccctgacca gaagaatcag agaagaattt catagattgg ttcagaagga tgggactcca
     8281 atttaagaaa tgatctgcac atcctgcggt aaaaacaccg cgagattttt ttatatggag
     8341 atttctgcgg gtgggaaggc aaccaccggt tgtttctgtt ttgattgcgt gaggaacaac
     8401 cctgagataa aaactgcttt tgaaacattt aagattcgac acaaaacaat ttcttacaaa
     8461 caaaaaaatt gtccattctg cggtatgaca cttgaagact ggaaacaatc ggggtttgcg
     8521 ggatgcgcaa catgttatca gattttcaga actcacataa aaagagaaat taagaagtgt
     8581 cagcatggtt cacttcatag agggaaattc cctcacagcg agaatgttag caagaatttt
     8641 ctggtatact ttgaaaatga aatgaggcgt gcggttgcag gtcgagacat tgaaaaggtg
     8701 aaaaaaatta agcaagaaat cgagatgttt ataaaaaatg gataaatatt tgagagacct
     8761 ggtattcaat gaacccctgt taagtgatga accgacattt tttactgcaa gattgcggct
     8821 tgcgagaaat attaaacaat atccatttcc gcatcaaatg agcgagaaac aggcagaaca
     8881 acttacgaga gaagtgattg acgtttctac cagtattacc gacgaagcgc ctgtgattat
     8941 ttccatgtca gatatagata atgtgaccag ggatgttctc atcgagaggc atattataag
     9001 tccggaaatg gcggaatatg gtttcgggaa gattctgata tggtttcctg aaatgaagtt
     9061 gcgaattttt gtgaatgaag aagaccattt cagaatttca atctgtggcg gaaaagaaga
     9121 cctctgcaat ctgtggaatg tattgaatca tgttgacgat attctatcca ggtgttttga
     9181 ttatgcgttt gataaagagt ttggatacct tacatgctgt ccgacaaatg taggacctgc
     9241 gcttagagta tcctcattgt ttttcttgcc agcgttaaaa atgactggaa aaataaaact
     9301 ggtgtttgat actatttcgc ggattggatg tataattaga ggattttatg gagaagggtc
     9361 aagaagtatt gcgaatgtct atcaaattgc aacaggacct gctcttggca aacaggaaat
     9421 tgaattgtgt caggattttg aatctgtgtc ccgcacaata aaaaaacagg aattgatgtc
     9481 gatgaacatc attgaccata tggcgataaa agggaaaatt gtctgtttcc tgaacaggat
     9541 ttttgaaaac aatagaggaa taaattcatc taattcaata aaaggccttt ctcttttgct
     9601 tttcggaaga aatttaggta ttatacatat gaatgtggta acattgaaaa cactgtttta
     9661 cagaatttta cccgcttctt tgcagttaga ggcaggtaca gagatggacg caatggagcg
     9721 ggacaatttg agattgcaga tattgagaaa cgaactcagg agcatatatg tttgagcagt
     9781 attttgaaag atttacgaaa agggtgaaga ctgtactgga acttgcacgc cagcatgcga
     9841 tacgatacgg gcatgctcag atagagacag aggatgttct tctcgggctt atagacgaag
     9901 gacagggaat gggtgttgct gtattgcatt tcctgaaaat tgaccccgaa catataagat
     9961 ttgagcttga aaaaagtatc agagtgggaa ttccattgct atcgtcggct gagattccat
    10021 tgagtcctgg agtgaaaaga gtgcttgcgc tttcttatcg ccaggcacaa tattttggac
    10081 attcttacgt tgggacagaa catcttctca tcgggcttgt ggcagaagag gaaggtcatg
    10141 ctgcaagggt gcttgtccgt tttggattga cagtggatag tatcagaaaa gccatagaag
    10201 gtcttctctg gggagaacct gaaaatgttt caaaagggaa acaacactca tcacagcaca
    10261 catcgcagcc tcagacaaaa tcaaaaactc cgactataga ttcgtatgga aaggatttaa
    10321 cgcagctggc tgaagggaaa aaacttgacc ctgtgatagg aagagaaaaa gaaattgaaa
    10381 gggttaccca gatactgtgc aggagaaaga aaaacaatcc tgtgttactg ggagaggccg
    10441 gtgttggaaa gacagctatt gttgaaggtt ttgcacagag aatagcatct ggcaatatcc
    10501 ctgagattct caagcataaa agaattatta tgattgacct tgccagtctt gtagcaggaa
    10561 caaagtaccg cggagaattc gaacagaggg taaaacttct gctggatgaa atcaagcaga
    10621 acaaaaacat catcattttt attgacgaac tccatacact tgttggcgca gggggtgctg
    10681 aaggggcgat agacgcgtca aatatattga agccggctct ttctcgtggc gagatacagt
    10741 gtattggagc aacgacattt gatgagtaca gaaagtatat tgaaaaggat gctgccctgg
    10801 aaagaagatt ccagccggtt gttatcaatc caccgacagt ccaggaaacc attgaaattt
    10861 tgtggggttt gcgccctaaa tatgaagaac atcataaact gaaaataatc gatgaagccc
    10921 tggtgcttgc tgcaaaactt gcagatagat acattactgg caggtatttg cccgataagg
    10981 caattgatct tgttgacgag gcggcaagca aacaaaggtt gttattttca aacacaccag
    11041 tggaacttga agaactggag aaaagagcag cgcagctggt gaatttgcag gaacaggcaa
    11101 caattcagca ggattttgaa aaggcagcaa gaataaggga tagattgaga gaaatacgaa
    11161 gtcgtatcaa tgaggtgaaa ctttcgtggg agaaagcaca acctgatacg catagaattg
    11221 tcacaggaaa agatattgcg gagattgttt caaaatggac agggattcct ttgtctcaat
    11281 tgtgcaagga agaaagggaa aaacttcttg aaatggaaga gcaccttcac agggttgtgg
    11341 tcggacagga cgaagcgatt tcagcggtca gcagagccat cagacgttca agggctggtg
    11401 taaaagacca gaaaagacct attggttcat ttttgtttct gggaccaact ggtgttggta
    11461 agaccctgct tgctcgtgcg ctggctgagt ttttgtttgg tgatgaccag gccctgattc
    11521 aaattgatat gtctgaatac atggaaaaat ttgctatcag caggttgata ggtgcgcccc
    11581 ctggttatgt gggatacgaa gaaggagggc aacttactga aaaggttcgc cgcagaccct
    11641 attcagtggt actgcttgat gagattgaaa aggcgcatcc agatgttttc aatttgcttc
    11701 tgcaggttct tgaagagggt agactgactg acagtttcgg caggagtgtc agttttcaga
    11761 acggcatact gatactaaca tcaaacattg gtacgcagca tttaaaaaac cgctccgcaa
    11821 ttggttttca ggaaaataag gaggagatac catatagcgc tgtgaaagca aggattctgg
    11881 acgagattaa aaagacattc agaccagaat ttcttaaccg ccttgatgag attatcgtat
    11941 ttcatcctct tgaacaggaa catctcctta aaattgttga ccttgaggta ggaaaagttg
    12001 ccgagaggtt aaaggaacag ggttttacaa tttccctcac ttctgaagca agggagtttt
    12061 tgaaaaatat gggcactgac cctcaatttg gagcaagacc tcttagaa
//
LOCUS       DAORYU010000016        11767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_4187_length_11767_cov_4.716274, whole genome shotgun sequence.
ACCESSION   DAORYU010000016 DAORYU010000000
VERSION     DAORYU010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11767
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4187_length_11767_cov_4.716274"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="PLW07_01090"
     CDS             <1..415
                     /locus_tag="PLW07_01090"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOL49151.1"
                     /translation="SGLGRVIHFAGLVFGVCLILFSLCKTFWLAVMILIIVGAAMVLQ
                     IVTSNIIIQTLVPDEKRGRVMSFHAIAFMGTQPAGSYLAGLLSKTMGIQNTVMLGGIL
                     CIIGSIVLKKTYTGFLRNLSDQDPQENLVKKIFNP"
     gene            complement(412..2091)
                     /locus_tag="PLW07_01095"
     CDS             complement(412..2091)
                     /locus_tag="PLW07_01095"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HOL49152.1"
                     /translation="MKKTSIRIACGQINTTVGDLTGNAGKIIDFIKQAQEIGVDIIAF
                     PELSITGYPPEDLLLKKSFVEDNIKILKSIASKTDDIVAIVGFVDMVQSNIYNAAAIM
                     NQRKIKAVYHKILLPNYGVFDEKRYFAPGDTGPVFKLQIQNTGCIFGISVCEDIWHNN
                     GPTGKQAKSGASFVININASPYHMGKIKEREKIAINQAKNNHLYMIYTNLVGGQDELV
                     FDGQSFVVSPSGKIISRAQAFEEQLLITDIEIETKKFRNNVQLVIKPKKKEPVPVFVA
                     KHLPVEEEVYRALVLGLKDYTEKNGFTKVVIGLSGGIDSALVATIAVDAMGKEKVKGV
                     FMPSRFTSEQSDIDATHLTENLGIELISIPIDEIFSSYLKTLEQCFKDAEPDITEENI
                     QARIRGNLLMALSNKFGYLVLTTGNKSEISTGYATLYGDMAGGFAVLKDVPKTLVYQL
                     ALYRNSQGKVIPDSIINKEPTAELRPNQKDTDTLPPYEILDKILKMYVEENRSIASII
                     KSGFEETIVRKVIKMVDKSEYKRRQAPPGIKITPRSFGKDRRMPITNKYQG"
     gene            2143..2724
                     /locus_tag="PLW07_01100"
     CDS             2143..2724
                     /locus_tag="PLW07_01100"
                     /inference="COORDINATES: protein motif:HMM:NF013988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMMECR1 family protein"
                     /protein_id="HOL49153.1"
                     /translation="MKSKFYVKKVTLLLFSTNIIPIFFANNAISLCRQSIEKYLTTGE
                     TIELKNLADTFVEKIPVFISLKKGNQTRGCAGTFSSENTFAENLVHFSIIAATQDFRY
                     RPVDIQELDDIRIQITIPQQPIEEIPCIEFYNPDTEGLIVKNQDKVGIVLPKEAKTSE
                     YALKIGLRNAGIKDTGGIKLFKFKAQVFMEERR"
     gene            2721..7847
                     /locus_tag="PLW07_01105"
     CDS             2721..7847
                     /locus_tag="PLW07_01105"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOL49154.1"
                     /translation="MKRMICVVVFAFASMCFCDTITGIVKTTDGKSVAGATIQIFKSR
                     YNEKNVKTSISDRYGKFTIDGLETGYYRLVASKKGFCDGNSGTIEVNQPKFGIASHEM
                     ILKKPGSISGFVYDPDGKPLQSAKLVATRNNKSTITDSRGFYKIDGLEPGTHYIYAEA
                     KGFVKNYVSSVVVEENKETAGINFTLSYAGAIKGVVVDAETGKSVEGVSVECDSIQGY
                     FSGKTDSNGRFFIDNLIPGKYNLSLYRQGYEYTNVSNITIVAQETTDAGQMKIKLRPK
                     YFNLYSSEWLFTPGENVKLHFNAYRISSVTMNIYEIDLLNEINRTKNFPRSIKEMLMS
                     VDISNQKPVLSKKIDISYKTPLTELSGRTIVAGTLPEGVYLCVITPENLPPQRQYFCV
                     TDVGFISKTCDNKSIVNVFSISKGIALADVSVYVFDHWWNLKQEIKTDSYGQFVLDTN
                     QKFLVKSGKSFAFSDIVYQNYYGSDDRRVLYAYTDRPVYRPNHTVYFKAIPRIDAGDK
                     YEMAHITSCQVKISAPDGSTLYETTLVPQKTGSIYGEWTIPDEPPLGTYSLNFTTQGK
                     DILSGNCVFKVLEYRKPEFSIEVKTDKLIYLPGEKINALISAKYYFGTPVKNTDVMWA
                     VYSKPSYNDGFEDYEGEYEYESWWRGALISSGNVKTDENGIAVIEIPTKKSYRQKEGY
                     SIEVKMTDLSRREITSSAKVLVVPGTFEISISTDKYVYSPDEEIPVSISARYYRDNSP
                     VIGNRFSISASQETYDQKKRRWTFKELLNKKFVCEGEKATIKIKPSATGYIKIDVQGI
                     DQFNNIISATRYVWIAGENYYSSWYGRKEIEIVSARKEYEVNDTAKILINSSYKDLNL
                     LFSIEGTRLFETKLIRMQGNSALIEIPIKQEYIPNIYISVCGVKNKNFISSSKPLKIA
                     CKEKVLNVEIIPEKTQLEPGDNAYYRIKITDSEEKPVSAEFSMGVVDESIYAISGELV
                     SKIEDFFYGSKRNRVSTFYSFNRWYYGGAGKDFSQQDIRRNFKDTAFWLPYAFTDSNG
                     ISDIQFNLPDNLTTWRTTIRAITSDTKVGSTINKIITKKPLIANLITPRFVVEDDRIL
                     ISGVIHNQTGKNQKVSVELKAEGLDILDEKEKTVEIENGQSSRVDWKVKVNSVKNAVI
                     TLFARSSTYKDAMELTLPVFSYGADERYVFAGRCDDTRIDTFHMPAGTIPASIDAKSY
                     IYPSLVSGLFTTLDELARYPYGCIEQTLSGFLPAVQVANTISRINKEDLDFLAGDKKR
                     FDEMMKQLPKKVSDGLIKLYNYQNQDGGWGWWSGDSSSPYTSGYVMFGLAHAKKSGYL
                     INEEKFELGKNFLKNAISSSQDYNQKVFMLYSLVYAGEKDASAVVEDIYKNRNKLHPY
                     TLAQLCLILQNLGDWRAKDLLNELCSKLIKPSPNYCYWQGEDGHYSWIHHNIETTAWG
                     LRAIIAIDPKRQEVPGILRYLVMKRQGGLWMSTKDTAMCLFAFTDYLEKSDELTPDYT
                     LLLLINNNEVAKEHITRESIKKFSTTINIPPENIKAGKTNDIKIQKQGTGNLYYTHLI
                     KYAVRDVVIPDFDSGFKVSRRYTALEPRKVQDASGNTVYVYDEITGPLKSGERFRVEI
                     KISGEEKYEYAMIEDPIPAGCEVVQEIPGDNWWYCRKEIRDEKVAFFTTFWYDKERTI
                     SYQLRAETPGFYHVLPTKAQLMYLPEIWGRSGSNTLIIAP"
     gene            7844..8515
                     /locus_tag="PLW07_01110"
     CDS             7844..8515
                     /locus_tag="PLW07_01110"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HOL49155.1"
                     /translation="MMKTFSRLVLLLSVSRLLCYAGEFKYQWASYTTYFRADIDQRQH
                     NINIAAMYLDGFVLCPKTIFSFNQEVTEKIPQEQFGPATILSGEQRTLGYGGGLCQVS
                     STLYAASLYAGLSIHERKPHSKPVNYIGIGLDATVSNDEGTDLKIFNPYNCELVIKAS
                     VTANALTVSIFGTRPKPKEINILVTKPEKKGNFLYTTTIRTVSSQGKKDFSEIVSRDR
                     YLLPE"
     gene            complement(8520..9500)
                     /locus_tag="PLW07_01115"
     CDS             complement(8520..9500)
                     /locus_tag="PLW07_01115"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOL49156.1"
                     /translation="MIYFIAISVKEQNLPVSGNQFIKYNKSMRRKKREHIADIFGYLF
                     FFGVFNIIRLLPSMLYPRIGKTTGKFFYIIAGSLKKKIRNNIIAAYGNQLSPEERTFM
                     TKKIIEKNTFFFIEWALWTKIHPQCALTLIEFKNIEPLMQVCQSKKPVILVSAHLGNF
                     ALMIAALIYAGLPVTWIARDANNQYLARYMDRIRRKKGVFGINKANLSQAIGLSLDWL
                     KQKKALCLLIDQHSGKGTEVCFFGKIVQAPTGAAVFARRFDASVFGVFIKRRKKFKHT
                     IFIEGPYNLAKTTNPEQDYQNNTQFFYNRIEYYVKQSPEEWFTWLHRRFR"
     gene            9583..10803
                     /gene="hisS"
                     /locus_tag="PLW07_01120"
     CDS             9583..10803
                     /gene="hisS"
                     /locus_tag="PLW07_01120"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOL49157.1"
                     /translation="MRDILPDETQRWKMLESLIFRALEGFGFEEIRVPVVEELKLFQR
                     SVGSFTDIVQKEMYVFKDRGDRVLALRPEATASVVRAYLEHKLYEKKRITRLYYWGPM
                     FRYDRPQKDRYRQFYQVGAEIFGGENAHFDAELIIILAKVFEKIGVADYIFEINSVGC
                     NQCKIDYSAQLKRFLQDRASHMCQDCQTRIQNNVLRVLDCKVVSCRSIIKEAPVIRNV
                     LCSRCKTHQETLYRILDSHKIPYRENQHLVRGLDYYTKTVFELKIADNDNAVAGGGRY
                     DNLVGELGGPETGACGFAIGMDRLCNMVSPEMKKVITVFVAFLGEQSLQQGVSIINQF
                     RNDHIKIVADYTDRSLKSHLKNADREGIQHVLILGENELQQEGFLYRNMKTGFQTIVK
                     FSELSQFLKELKDA"
     gene            10796..>11767
                     /gene="aspS"
                     /locus_tag="PLW07_01125"
     CDS             10796..>11767
                     /gene="aspS"
                     /locus_tag="PLW07_01125"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOL49158.1"
                     /translation="MLRTHTCGQLNLDAVGKTVVLAGWVSSIRDHGNIIFFDLRDRYG
                     LTQIVCNLPQMSESLVEKVKKIRQEFVIRISGNVVARAPETVNPKLSTGSIEVIAEDV
                     EILNSCDPLPFEIVESKTINENTRLKYRYLDLRRDDLKENIVFRSRFTGRMREFFSSE
                     RFVEIETPILTKSTPEGARDFLVPSRLNPGNFYALPQSPQLFKQILMVSGFDRYYQIA
                     RCFRDEDLRSDRQPEFTQVDIEMSFIEENDIIDVCERLLKHAIEKTTGIEIKIPFPRL
                     SYEDAINRFGTDKPDLRAGMEIVDLSELFANSGSSILEGILQAGLIIK"
BASE COUNT         3744 a         1990 c         2409 g         3624 t
ORIGIN      
        1 ttctggactt ggtagagtca ttcattttgc aggacttgtt tttggtgtat gtcttattct
       61 attttcgctt tgcaaaactt tctggctggc agtgatgatt ttaattattg ttggcgcagc
      121 catggttctt caaattgtga catcaaatat tattattcaa acgcttgtgc ctgacgaaaa
      181 gaggggaagg gtgatgagtt ttcatgcaat tgcttttatg ggcactcaac ctgctggttc
      241 atatcttgca ggattattga gcaagacaat gggcattcaa aatacagtga tgcttggtgg
      301 gattctgtgt attattggaa gtattgtgtt gaagaaaact tatacgggtt ttttaagaaa
      361 tttaagtgac caggacccac aagaaaattt ggtaaagaaa atttttaacc cttaaccctg
      421 atatttattt gtaatcggca ttcttctatc tttcccaaat gaacgcggcg taattttgat
      481 gcctggcgga gcctggcgtc tcttatattc gcttttatct accattttta tgacttttct
      541 gacaatggtt tcttcaaatc cggatttaat aatgctggcg atacttctgt tttcttcaac
      601 gtacattttt aaaattttat caagaatctc atagggcggg agcgtatcag tgtctttctg
      661 gttgggtctg agttctgctg ttggttcttt attgatgata ctatctggaa tgacttttcc
      721 ctgactgttt cggtacaatg cgagttgata aaccagtgtt ttcggtacat ctttaagaac
      781 agcaaaacct ccagccatat ctccgtatag ggtcgcgtat cctgtgctga tttctgattt
      841 attaccggtt gtgagaacca ggtatccgaa tttgtttgac agtgccatca ggagatttcc
      901 ccttattctt gcctgaatgt tttcttcagt aatatcgggt tctgcatctt tgaaacactg
      961 ctcgagtgtt tttaggtaag atgaaaaaat ttcatcaatg ggaatactta tcagctcaat
     1021 gccaagattt tctgtaaggt gtgttgcatc aatgtcagat tgttctgatg tgaatcgcga
     1081 aggcatgaaa acgcctttta ccttttcttt tcccatggcg tcaacagcaa ttgttgcaac
     1141 aagcgcagaa tctattcctc cactgagtcc gatgacaacc tttgtgaatc cgtttttttc
     1201 tgtataatcc tttaatccaa gaacaagagc gcggtacact tcttcttcaa ctggaaggtg
     1261 ttttgcaaca aacacaggca ctggttcttt tttcttcggt ttaatgacaa gttgaacatt
     1321 atttctaaat ttttttgttt ctatttcaat gtcagtgatc agaagttgtt cctcgaacgc
     1381 ctgtgcccgt gaaatgattt ttcctgaagg tgaaacgaca aaactctgtc catcaaacac
     1441 aagttcatcc tgtccaccaa ccagattggt gtaaatcatg tacagatgat tgttttttgc
     1501 ctgattgatt gcgatttttt ctctttcttt aatttttccc atgtgatatg gggatgcatt
     1561 gatattgatg acaaacgatg ctcctgactt tgcctgtttg ccagttggac cattattgtg
     1621 ccagatgtct tcgcagacac tgattccgaa aatacagcca gtattttgta tttgaagttt
     1681 aaaaacagga cctgtatcac caggtgcaaa atatcttttt tcgtcaaaga ccccgtaatt
     1741 tggtagcaat attttgtgat aaaccgcttt gatttttctc tggttcatga tggcagcagc
     1801 attataaata tttgattgta ccatatcaac aaagcccacg attgcgacaa tgtcatctgt
     1861 tttacttgca atggatttta aaattttgat attatcttca acaaaagatt ttttgagtaa
     1921 aagatcttca ggtggatatc ctgtaatgct aagttcagga aaagcaatga tgtcaacccc
     1981 gatttcctgc gcttgtttta taaaatcaat aattttgcct gcattgccag tcaaatcacc
     2041 aactgtggta ttgatctgac cgcacgcgat acgaattgag gtttttttca tgttatttgc
     2101 ctttttgtaa aacacggttt ttatagtata cttctatcac aaatgaaatc caaattttat
     2161 gtaaaaaagg ttactttgtt actattttcc acaaatataa tccccatttt ttttgcaaat
     2221 aatgcaattt ctttatgcag gcagtcaatc gaaaaatacc tgacaacagg tgaaacaatt
     2281 gaattgaaaa atctcgctga tacctttgtt gaaaaaattc ctgttttcat ttctttgaaa
     2341 aaaggaaatc aaacaagagg ttgtgctggt actttttcat cagaaaatac ttttgctgaa
     2401 aatcttgttc atttttccat tattgcggca acccaggatt ttagatacag accagttgat
     2461 atacaggagt tggatgatat cagaatacaa attacaattc cgcagcagcc aatagaagaa
     2521 ataccctgca tagaatttta taatccagac actgaaggac tgattgttaa aaaccaggat
     2581 aaagtgggaa tagtgttgcc aaaagaagca aaaacctctg agtatgcgtt gaagataggt
     2641 ttaagaaacg cagggattaa ggatacaggc ggaataaaat tgttcaagtt caaggcacaa
     2701 gtttttatgg aggagagaag atgaaaagaa tgatctgtgt tgttgtattt gcttttgctt
     2761 ccatgtgttt ttgcgacacg attactggta ttgtaaagac cacagatggt aagtccgttg
     2821 ctggtgctac aattcaaatt tttaaaagca ggtacaatga aaaaaatgta aagacatcca
     2881 tttctgatag atatggaaaa tttaccattg atggtcttga aaccggttat tacaggttgg
     2941 ttgcgtcaaa aaaaggattt tgtgatggta attctggtac cattgaagta aaccagccaa
     3001 aatttggaat tgccagtcat gagatgatac taaaaaaacc tggttcaatc agtggttttg
     3061 tttatgaccc ggatggaaaa ccattgcaat ctgcaaagct ggttgccaca agaaataaca
     3121 aatccacaat cacagatagc aggggctttt ataaaattga tggactggag ccaggtaccc
     3181 attacattta tgcagaagca aaggggtttg ttaaaaacta cgtgtcttct gtggttgttg
     3241 aggaaaacaa agaaacagcg ggtatcaatt ttactctttc atatgcagga gcaatcaagg
     3301 gagttgttgt tgatgctgaa acaggcaaat ctgtagaagg tgtcagtgtt gagtgtgata
     3361 gcattcaagg gtatttttct ggaaaaacag attcaaatgg cagatttttt attgacaatt
     3421 tgattcctgg aaaatataac ctttcattat acaggcaggg ttatgaatac acgaatgtga
     3481 gtaatattac catagttgct caggaaacca ctgatgctgg acagatgaaa atcaaactca
     3541 gaccaaaata ctttaatctg tactcaagtg aatggctttt tactcctggt gaaaatgtta
     3601 aacttcattt caatgcgtat agaatttcct ctgtaacaat gaatatttat gagattgacc
     3661 ttttgaatga aataaacaga acaaaaaatt tccctcgctc aatcaaagaa atgttgatgt
     3721 ctgttgatat atcaaatcaa aagcctgtat taagcaagaa aattgatatt tcctataaaa
     3781 cccccttaac cgaattatca ggtagaacaa ttgtagcagg gaccttgcct gaaggtgtat
     3841 atttgtgtgt gataacacca gaaaatttgc cgccacagag acagtatttt tgcgtaactg
     3901 atgtgggttt tatttcaaaa acgtgtgata acaaatctat tgttaatgtt ttttcaattt
     3961 caaaaggtat tgctcttgcg gatgtgtctg tttatgtttt tgaccactgg tggaatctga
     4021 agcaggaaat caaaacagat agctatgggc agtttgttct tgatacaaac cagaaatttc
     4081 tggtgaagtc aggaaaatct tttgcgtttt cagatattgt ttatcaaaat tattatggaa
     4141 gtgatgatag aagagtgcta tacgcatata ctgatagacc tgtgtataga ccaaatcata
     4201 cagtttattt caaagcaatt ccaaggattg atgccggtga taaatatgaa atggcgcaca
     4261 ttaccagttg tcaggtaaaa ataagtgcgc cagatggaag cacattgtat gaaaccaccc
     4321 tggtaccgca aaaaactggt tcaatttatg gcgagtggac aatccctgat gagccaccac
     4381 ttggcactta ctcattaaac ttcactacac aggggaaaga tattttaagt gggaattgtg
     4441 tgttcaaagt tcttgaatat agaaagccag agttttccat tgaagtgaag acagacaaat
     4501 tgatatatct ccctggagaa aaaatcaatg cactgatttc agcaaagtac tattttggaa
     4561 cacctgtaaa aaatacagat gttatgtggg ctgtgtattc aaaaccatcg tacaatgatg
     4621 ggtttgagga ttatgaagga gaatatgagt atgaatcatg gtggcgcggc gctcttatta
     4681 gttcaggcaa tgtgaaaacc gatgaaaatg gaatagcagt gattgaaatt ccgacaaaaa
     4741 agtcataccg gcaaaaggaa ggttattcaa ttgaagtgaa aatgactgac ttaagtagaa
     4801 gagaaatcac atcaagtgca aaagttcttg ttgttcctgg tacttttgaa atttcaattt
     4861 caacagataa atatgtttat tcgcctgatg aagaaatacc tgtgagtatt tcagcaaggt
     4921 actaccgaga taacagtcca gttattggaa accgcttcag tatttctgcg agtcaggaaa
     4981 catacgacca gaagaagaga agatggactt tcaaggaact tttgaataaa aagtttgtgt
     5041 gtgaaggaga gaaagcaaca ataaaaataa agccatcagc aacaggttat atcaaaattg
     5101 atgtgcaggg aatagaccag tttaacaata tcatttctgc caccagatat gtatggattg
     5161 caggcgaaaa ttattattcg tcatggtatg gaagaaaaga aatagaaatt gtttcagcca
     5221 ggaaagaata tgaggtgaac gacacagcaa agatccttat caattcttct tacaaagatt
     5281 tgaatctttt gttttcaatt gaaggcacaa gattatttga aacaaaactt ataagaatgc
     5341 agggaaattc agcactgatt gagataccaa taaaacagga gtatatacca aacatctaca
     5401 tttcagtatg tggcgtgaaa aacaaaaatt ttatttcttc aagcaaacca ttaaagattg
     5461 cctgtaaaga aaaagttctt aatgttgaaa tcattccaga aaaaactcaa cttgagcccg
     5521 gtgataatgc ttattacaga ataaagatta ctgattcaga agagaaacct gtttctgctg
     5581 agttttctat gggggtagtg gacgaatcaa tttatgcaat ttctggtgaa cttgtttcaa
     5641 aaattgaaga ttttttctat ggcagcaaaa gaaacagggt ttcaaccttt tattctttta
     5701 atcgctggta ttatggtgga gcaggaaaag atttcagcca gcaggatatc agaagaaatt
     5761 tcaaggatac tgctttctgg ctgccttatg catttactga ttcaaatggc atatcagata
     5821 ttcagtttaa ccttcctgac aatcttacga catggagaac aacaattcgt gccattactt
     5881 ctgatacaaa agtcggttca acaattaata aaatcattac caaaaaaccc ctgattgcaa
     5941 atctcatcac accaagattt gtggttgaag acgacagaat cttgatttca ggagtcattc
     6001 ataatcagac agggaaaaat caaaaagttt ctgttgaact gaaagcagaa ggacttgata
     6061 ttcttgatga aaaggaaaaa actgttgaaa ttgaaaacgg gcaatcatca agggttgact
     6121 ggaaagttaa agttaattca gtgaaaaatg ctgtgattac cctttttgct cgctcatcaa
     6181 cctataagga tgcgatggaa ttaacgcttc ctgtgttttc gtacggtgct gatgaacgat
     6241 atgtttttgc gggcagatgc gatgatacaa ggatagatac atttcatatg ccagcaggga
     6301 caattccagc atccattgac gcaaaatctt acatttatcc gtctttggtt tctggtcttt
     6361 ttacaactct ggacgaactt gccagatatc cttatggatg tattgagcaa acgctcagtg
     6421 gatttttacc tgctgtgcag gtggctaata cgatttcaag aatcaacaaa gaagaccttg
     6481 attttcttgc gggtgataaa aaaagattcg atgagatgat gaaacaactt ccaaaaaagg
     6541 tgagcgacgg actgataaag ctttataact atcaaaatca ggatggtgga tggggatggt
     6601 ggtcaggtga ttcaagtagt ccctatacat caggttatgt aatgtttggg cttgctcacg
     6661 caaaaaaatc cggttatctc atcaatgaag aaaaatttga attgggtaaa aattttctca
     6721 aaaatgcaat cagttcatct caggattaca accagaaggt tttcatgctt tattctcttg
     6781 tgtacgcggg tgaaaaagat gcttccgcag tagtagaaga tatttataaa aatagaaaca
     6841 aacttcatcc atatacattg gcgcagttgt gcctgattct tcaaaatctc ggcgattgga
     6901 gagcaaaaga cctacttaat gaactttgca gtaaactgat aaaaccgtct ccaaattatt
     6961 gttactggca gggagaagat ggtcattaca gctggattca tcataatatt gaaaccacgg
     7021 catgggggct cagggcaatt attgcgattg acccgaaaag gcaggaagta ccgggtattt
     7081 tgagatatct tgtgatgaag agacagggtg gattgtggat gtcaacaaaa gatacagcga
     7141 tgtgcctttt tgccttcaca gattatcttg aaaaaagtga tgaacttaca cctgattaca
     7201 cattactgct tttaatcaac aacaatgaag ttgcaaaaga acatattaca agagaaagta
     7261 taaagaaatt tagcaccact attaatattc ctcctgaaaa cataaaagca ggcaaaacca
     7321 atgatattaa aattcaaaaa cagggcacag ggaatcttta ctatacgcat ctcattaaat
     7381 atgctgtgag agatgtagtg attcctgatt ttgatagcgg gttcaaggtg tcgagaagat
     7441 acactgcgct tgaaccccgt aaggtgcagg atgccagcgg aaatacagta tatgtctatg
     7501 acgaaattac aggaccattg aaaagtggag aaagattcag ggtggaaata aaaatttctg
     7561 gtgaagaaaa atatgaatat gcgatgatag aagacccgat tccagcaggt tgtgaagttg
     7621 ttcaggaaat accaggtgat aactggtggt attgtagaaa agaaatcagg gatgaaaagg
     7681 tggcgttttt caccacgttc tggtatgaca aagaaaggac tatttcatat caattgaggg
     7741 ctgaaacacc tggtttttac catgtattgc cgacaaaggc acagttgatg tatctacctg
     7801 aaatatgggg tcgttcagga agcaacacat taataattgc gccatgatga aaactttctc
     7861 cagacttgtg ttactattat cagtttctag gctgctttgt tacgcaggtg aattcaaata
     7921 tcagtgggct tcatatacca catatttcag agccgatatt gaccagaggc aacacaatat
     7981 caacattgct gcaatgtatc ttgatggatt tgtgctttgt ccaaaaacaa ttttttcgtt
     8041 caatcaagaa gtgactgaaa aaattccaca ggaacaattt ggccctgcta cgattctttc
     8101 aggtgaacaa agaacattgg gatacggcgg tgggctttgc caggtatctt caacccttta
     8161 tgctgcgtct ctttatgcag ggctttccat tcatgaaagg aaaccacatt caaaaccagt
     8221 aaactatatt ggcattggac ttgatgccac agtgtcaaat gatgaaggga cggacctgaa
     8281 gatttttaat ccctacaatt gcgaacttgt gataaaagca tctgttactg ccaatgctct
     8341 cactgtatca atttttggca ccagaccaaa gccaaaagag ataaacattt tggttacaaa
     8401 accagaaaag aaaggaaatt ttctttatac cacaacaata agaactgttt cttcacaggg
     8461 taaaaaagat ttttcagaaa ttgtttcacg agacaggtat cttttgccag agtgattttt
     8521 tatctgaatc ttctgtgtaa ccaggtgaac cattcttcag gactctgttt tacataatac
     8581 tctatacgat tgtagaaaaa ctgagtattg ttttgataat cctgttctgg atttgttgtt
     8641 tttgcaagat tgtagggacc ttcaataaaa atcgtgtgct tgaatttttt tctgcgcttg
     8701 ataaacacac caaaaacact tgcgtcaaac cttcttgcaa aaacagcagc tcctgttggc
     8761 gcctgcacaa tttttccaaa aaaacaaact tctgtacctt ttcctgaatg ctggtcaatc
     8821 aaaaggcata atgctttttt ttgtttcagc cagtcaaggg ataaaccaat tgcctgagaa
     8881 agattggctt tattgatgcc aaatactcct tttttccttc tgatcctatc catatatctg
     8941 gcaagatact gattgtttgc atcccttgct atccatgtga ccggtaatcc cgcataaatc
     9001 agggcagcaa tcatcagcgc aaaattgcca agatgagcgc tgacaagtat cacaggtttt
     9061 tttgattgac atacctgcat taagggttca atatttttga attcaatcaa tgttaatgcg
     9121 cactgtggat ggatctttgt ccataacgcc cattcaatga aaaaaaaggt atttttttca
     9181 atgatttttt ttgtcataaa ggttctttct tctggagaca attgattgcc ataggcagca
     9241 ataatgttat ttcttatttt ctttttcagt gaacctgcga tgatataaaa aaatttgcct
     9301 gtggttttgc ctatccgtgg ataaagcatt gaaggcaaca atcttatgat gttgaaaaca
     9361 ccaaaaaaga ataaatatcc gaatatatct gcaatgtgtt cacgtttttt tcttctcatg
     9421 gacttattgt actttataaa ctgatttcct gaaaccggta gattttgttc tttgaccgat
     9481 atcgcaataa aatatatcat atacagaata agccacaggc gcaaatttac actgttaaca
     9541 aaatttattg atatgaaaga gcaaataaaa tcagtgaggg gaatgaggga tattcttccg
     9601 gatgagacgc agcgctggaa gatgcttgag tcattgattt tcagggctct tgaaggattt
     9661 gggtttgagg aaataagagt tccagtagtt gaagaactga aactgtttca gagaagtgtt
     9721 ggttctttta ccgatattgt tcaaaaagaa atgtatgtgt tcaaagacag gggagacagg
     9781 gttcttgctc tcagaccaga agcaactgct tctgttgttc gtgcatatct cgagcacaaa
     9841 ctctacgaaa aaaagagaat cacgcgactt tactattggg gtcctatgtt caggtatgac
     9901 aggccacaaa aagataggta cagacagttc tatcaggttg gtgcagagat ttttggtgga
     9961 gagaatgccc attttgatgc agaacttatc ataatactgg caaaagtttt tgaaaaaatt
    10021 ggtgtggctg attatatttt tgaaattaat tcagttggtt gtaatcaatg taagatagat
    10081 tacagcgcac aattgaaacg atttcttcag gatagggctt ctcatatgtg tcaggattgt
    10141 cagacaagaa tccagaataa tgtgctacgt gttctggatt gcaaggttgt ttcctgtaga
    10201 agcattataa aagaagcgcc tgttattcgg aatgtacttt gttccaggtg taagacacat
    10261 caggaaaccc tttacaggat tcttgatagt cacaaaattc catacaggga aaaccagcat
    10321 cttgtcaggg ggcttgatta ttacacaaag acagtgtttg agttaaaaat tgcagacaac
    10381 gacaatgcag tggctggtgg aggacgatac gacaatcttg ttggtgaact tggtggtcct
    10441 gagacaggtg cgtgtgggtt tgccataggg atggatagat tgtgcaatat ggtttctcct
    10501 gaaatgaaaa aggtcattac agtatttgtt gcttttcttg gcgaacaatc cctgcaacag
    10561 ggagtgagca tcatcaatca attcagaaat gaccatataa aaatcgttgc ggattatact
    10621 gataggtctc tcaaaagcca tctgaaaaat gctgataggg aaggtattca gcatgttctt
    10681 attcttggcg aaaacgaatt gcagcaagaa gggtttcttt acagaaatat gaaaactgga
    10741 tttcaaacaa ttgtaaaatt ttctgaactt tcacaatttt tgaaggagtt gaaggatgct
    10801 tagaactcat acatgtggac aattgaatct tgatgctgtt ggtaaaacgg ttgttcttgc
    10861 aggctgggtt tcttcaatac gagaccatgg taatatcata ttttttgacc tgcgggatcg
    10921 ttatggactg actcaaattg tatgcaatct tccccagatg tcagaatctc ttgttgaaaa
    10981 agtaaaaaaa ataagacagg aatttgttat tcggatttca ggaaacgttg ttgcccgtgc
    11041 gccagaaaca gtcaatccca aactttccac tggttccatt gaagtgattg ccgaagatgt
    11101 ggaaattttg aacagttgcg acccacttcc ctttgaaatc gtggagagca agaccataaa
    11161 cgagaacaca cggctcaaat ataggtatct tgatttaaga agagatgatt tgaaagaaaa
    11221 catagtgttc aggtccaggt ttacaggtag gatgagggaa tttttttcct ctgaaaggtt
    11281 tgttgaaatt gaaacgccaa ttcttacaaa gagcacgcca gaaggcgcaa gggattttct
    11341 tgtgccatca aggttaaatc cgggtaattt ctatgcgctt ccacagtcgc cacaattatt
    11401 caaacagatt ttgatggtat ctggttttga tagatattat caaattgccc gttgtttcag
    11461 ggatgaggac cttcgttctg acagacagcc agaatttact caggttgata ttgaaatgtc
    11521 atttattgaa gaaaacgaca tcatagatgt ctgcgagcgc ttacttaaac atgctattga
    11581 aaaaacaaca ggtatagaaa taaaaatacc atttccaaga ttatcatatg aggatgccat
    11641 caacagattt ggcacagaca aacctgacct tcgggcagga atggagattg ttgacctttc
    11701 agaacttttc gctaattctg gaagcagcat tcttgaaggt atcctgcagg caggactcat
    11761 tattaaa
//
LOCUS       DAORYU010000017        11677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_4222_length_11677_cov_3.947771, whole genome shotgun sequence.
ACCESSION   DAORYU010000017 DAORYU010000000
VERSION     DAORYU010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11677
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4222_length_11677_cov_3.947771"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..1666
                     /gene="trpE"
                     /locus_tag="PLW07_01130"
     CDS             179..1666
                     /gene="trpE"
                     /locus_tag="PLW07_01130"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HOL49159.1"
                     /translation="MEIYPEFSEFEKLAGNYNLVPVWTEFLADLETPVGVFMKLTSNS
                     ETNFLLESVEQGGKLGRYSFIGIQPRELFKSTGNTVEFVNEEGNVKKITTIDPLSELE
                     KIVSYYRQPEDVRLPAFAGGAVGYISYDYVRFIEKLPEILPSDFLFPELYFQMCFDLV
                     VFDHLKRKIQVISNAKIKKGQSLKNSYKKSCEKIESIISMIRKPIPYFRNNSAVDAGK
                     FISNYKQDEFESAVTKAKKYIRAGDIIQVVLSQKWERNIDVSPLSVYRSLRSVNPSPY
                     MFYLNFPGIYLVGSSPEILVRCNGSDALLRPIAGTRPRGKDDAEDEKLAEELKKDIKE
                     IAEHIMLVDLGRNDLGRVCRFGSVKVTDLMFVEKYSHVMHLVTNVVGKLAKGKNYFDL
                     VRACFPAGTVSGAPKIRAMEIIEELEKSRRSVYAGAVGYFGFHSDMDFCITIRTMFGS
                     ADKIYIQAGAGIVADSVPEKEYIETINKAKALMLAYENAKKMDAI"
     gene            1679..4915
                     /locus_tag="PLW07_01135"
     CDS             1679..4915
                     /locus_tag="PLW07_01135"
                     /inference="COORDINATES: protein motif:HMM:NF020121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HOL49160.1"
                     /translation="MKVILRAGLMILMAVFVVNCQGKNNVRIIVEAEDMTGVNQKNFG
                     PGQTWNIGRWGIDLYQNMTFGGLWASRLKTAITDEKNGDAEISKIIEIPQSDTYKVWV
                     KYECPPFFNYAFRVRIIDRAGNTVFDKIYGLLTSEKHYCFTDKVVRGSLYWQWGIDHD
                     AAEGYDVKLDKGTYKLSISKTHNPEPAGCRSIDVVMITNDLSELSAPKYPRYPLLDEL
                     RRENHVYFRFRNLSEKPIAVKWNHWNHRYPDFYSPMYRDLVRFYDENGKQVAKEGMKF
                     TGDWPEPIQPGKVSVWYDLGPTMNVESTSPYTFKATSVDGSIKNPRFAVEIALEPNEK
                     KIVKSFEISQDEEELTILVQPDLYTKEGVAFTKKIADIFRDITMQLNKEKRIGPIPKK
                     IRLFAYTGRVTDQPNSKWGFEIEQSFRHALGINTIGYVNDGNEAKKVIEWWKDKGGVV
                     EQSYSYHHSQDIPKVVKMIKDKQLEPYFYYLSYGDEIGLPVSKADDPKLLEQFRDFVK
                     KHGEIPESLGCEYWENVKPVNSPSSEIALQIGVISDKKDAVSVSQTMKKLYWYSVLFQ
                     EELGIRDFAEKTKQLKKELGEHVHTSANLGGMHPFYWVHQQSFIEAFKHGAMSLAWSE
                     DYTYCMPEASALVIDFLASYLRKGASYHDTPMQFYCMPHYPGCPPDLLLKNAVLLWAN
                     NVKDLDFFSAGFDGYMTENYIAYRVGLPTFKAIRTISGMAGLIEDYLVPARVEKTPVA
                     MLLSKSSDVWEIEGKSQWDIKPGSHATNVFQEERKNIWYALRKAGYRVDFITENDIKE
                     GILDRYRVLYIACQNIEKQPAERINQWVRNGGIVFATAASCRRDEFDQPLSTLDETFG
                     RGKQISYQQYKGPLRAKLELLFQLPLDYIIFDNQSSKVLCNKEVFEVAPDAEVIARYC
                     SDGMPAWIKKKTGSGLVYYGGTLPGQSYIQYGLKVLPCGKGGVNQSFCHFGAGNLEPV
                     QNDPVSEDLILLPLRENGILPDVITDRKGIVSGRLASENATVIPLVNLGESTSMKNIS
                     INVNSLNYNPKKVWSCFYPKGLKFSVNNSQVSIKIPSMGAADVIVIER"
     gene            4917..5999
                     /locus_tag="PLW07_01140"
     CDS             4917..5999
                     /locus_tag="PLW07_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49161.1"
                     /translation="MSLLYKPDWHETKENYRKWWAHEYFGRCAIAVYAPKKNAPDIPA
                     PPEPESIHQKWYDLDYISQVNYYNLSRTFFGAEALPIWNGGYPGHTAIPTFLGCEVHL
                     DMRTGWWEPILKQEDLTFEHLEIDENHPDYRFSIELLKRAIVEAQGKCLVSIGAFGGC
                     GDTLAALRGTERLLFDCIERPERVREAELYLTEMWCRHYEKLYNIIKKVDEGSTCWFP
                     LWAPGKFYASQNDFAYNISPSMFREVFLPAIKKQVEFLDYTIYHVDGIGNFNHIDALL
                     ELPELQAIQILPGAGKPSPLHFIGILKKVQKEGKNLHIAIPAGEVKTALEHLSARGLF
                     ISTSCETEDEALRLIENTYRWSEDRS"
     gene            6054..6623
                     /locus_tag="PLW07_01145"
     CDS             6054..6623
                     /locus_tag="PLW07_01145"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012962726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOL49162.1"
                     /translation="MQTILMIDNYDSFTYNLVQYLGELGCRIKVFRNDRISIEKIKKM
                     KFDKIVISPGPGRPENAGISVELIKVFEDKKPILGVCLGHQCIGYLYGGKIVGARYLM
                     HGKTSLIYHKDENIFKNISNPFSATRYHSLVIEKNSSPDVLKITAWSDDGEIMGICHR
                     KYGIFGVQFHPESVLTDNGKKILKNFLEL"
     gene            6620..7633
                     /gene="trpD"
                     /locus_tag="PLW07_01150"
     CDS             6620..7633
                     /gene="trpD"
                     /locus_tag="PLW07_01150"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009005354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOL49163.1"
                     /translation="MNIDEILTKAVSGQPITRIESAEIFRQIMSGNVSPVKMASFLTA
                     LRVSGETGEVVAGAVEVMREKCLKVKSTGIVADTCGTGGDHTGTFNISTASAIVGSAC
                     GVKVAKHGNRAVSSKSGSADVLEKLGMNIEISPEKAEMMLEKVNFTFLFAPLYHPAMK
                     NVAAVRKELGFRTIFNILGPLCNPAGASVQILGVGNKNLLDIVPDVLSGFNVKRAWIF
                     YGEDGTDEISITGKTTVIEIGETRKKFSIEPEEFELKKATLKELEGGSPEENAEILRL
                     IISGKMKGAKRDAVILNAGALLYLAGVAGSVIDGMKTAQEVIDSGYCTGHLEKIISMS
                     KEN"
     gene            7578..8240
                     /locus_tag="PLW07_01155"
     CDS             7578..8240
                     /locus_tag="PLW07_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49164.1"
                     /translation="MDIVPDTLKKLFLCQKKTKLFFFFLILPLGVCAQPEQVAIQLLD
                     ITNQREPVINIINLKIDQLWHSGRYEKIFPLFHLVVRLDPLDEEAWATGGWFLIYCVA
                     PMTGFPESEKIKQKGIEFLKEGLTHNFDSYRLYWELAWIYYARGEFGNAIAYLDKAIQ
                     YEHPAYVENTRAHALEKLGRIDDAIKQWQEIKKKFPEMRFVAERFILNLQKEKTDVKQ
                     NP"
     gene            8221..9015
                     /locus_tag="PLW07_01160"
     CDS             8221..9015
                     /locus_tag="PLW07_01160"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOL49165.1"
                     /translation="MLNKILEHKRNVVEQKKLSIPLDVLKQRIQPTVCRSVIERTKQQ
                     PFIIIAETKKASPSGGIFRKKYSPSKIAASYEKGGASAISVLTEDRYFLGSPRHLQSV
                     KNTVSVPVLAKDFFIDPYQIYEAKMYGADCILLIIRILSDEQFISLLSVARTLNLEIL
                     VELHTEKEIERFFNIMPASKGILVGINSRDLDSLRIDLNITIYLISLLKGVKIPIIAE
                     SGVNDIKTVIDLYNAGASGALIGSRFLKEKSPGISVTKFLKEIYDG"
     gene            9008..10366
                     /locus_tag="PLW07_01165"
     CDS             9008..10366
                     /locus_tag="PLW07_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HOL49166.1"
                     /translation="MDERKILLSEKEMPVAWYNVLPDLPEQLPPPLDPRTGKPVDPSV
                     LAAIFPVSLLQQEMSVEPWIEIPEEIRQVYAMWRPTPLHRAVALEKYLKTPARIYFKN
                     ESVSPPGSHKPNTAVAQAYYNKKEGVKRLCTETGAGQWGSALSFACNLFGLECVVYMV
                     RVSYQQKPYRRIMMQTWNAKIYPSPSDQTNYGRKLLKENPDNPGSLGIAISEAIEDTI
                     SGKDSKYSLGSVLNHVLLHQTVVGLETQKQLELIGEKADVLVGCVGGGSNFAGLVLPF
                     VKDKIKGKSEVEIIGVEPTACPTMTKGPYRYDYGDTSGLTPLLKMLTLGHDFMPPGIH
                     AGGLRYHGLAPIVSMLVDKKIVRAEAVAQTKAFEAAVIFARTEGFLPAPETSHAIRVV
                     IDEAKKCAETGEEKCIVFNFSGHGHFDLASYDAYLAGKLQDYEYPEEKIRESLEKLPK
                     IE"
     gene            10363..11001
                     /locus_tag="PLW07_01170"
     CDS             10363..11001
                     /locus_tag="PLW07_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOL49167.1"
                     /translation="MKVKICGITQRKDAEQCDALEVDYLGFNFYRLSKRYILPEKARD
                     IIPFLKRAKPVGVFVEQDLDYIKGIVDMCNLYGIQIHGSENSEFCYRIKKLFPDRMVI
                     QVFRIKETIPSNLNDFDADYYLFDSFDDSSPGGTGKTFDWTVLQKMESIAQRSFIAGG
                     INPENVKALISCVDVYGIDVASGVEKVPGIKDMEKVRQLLVKTKGCVNDKIT"
     gene            10985..>11677
                     /locus_tag="PLW07_01175"
     CDS             10985..>11677
                     /locus_tag="PLW07_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOL49168.1"
                     /translation="MTKLPDRRGRFGIYGGKFAPETLMAPIEELEHVYFTTGTSKQFR
                     KELDCYLKNYAGRPTSLYYAESFSKEIGCRVYLKREDLAFTGAHKINNTIGQVLLAKK
                     MGKTRVIAETGAGQHGVATATAAALFKLECEIYMGELDMQRQSLNVYRMRLLGAKVNC
                     VKSGSRTLKDAINEALRDWISNFKTTHYVIGSVVGFHPFPVMVRDFQSVIGKETRRQI
                     LRAEKRLPDYLVA"
BASE COUNT         3789 a         1929 c         2647 g         3312 t
ORIGIN      
        1 aaccatcaac cccagcaaaa tcaaaagaaa tccagcaaca ataagaaatt ttgccatcag
       61 taaatattct accatagtct tgcgtatccg ttgagttgcg atggtcccag gtggcgtctt
      121 gttgcctgca tctctggaat gtgataaagt tatattcatc ttaaacactg gaataaatat
      181 ggaaatctat ccagaattct cagaatttga aaaactagca ggaaactaca atcttgttcc
      241 tgtatggaca gagtttcttg ctgaccttga aacacctgtt ggtgtgttta tgaaattaac
      301 gtctaattca gagacgaatt ttcttcttga aagcgttgag cagggtggaa aacttggcag
      361 gtactcattt attggcattc aaccaaggga attattcaaa agtactggaa atactgttga
      421 atttgtaaat gaagaaggta atgtcaaaaa aatcaccaca attgacccgc tctcagaact
      481 cgagaaaatc gtcagttatt acaggcaacc tgaagatgtt cggcttcctg catttgcagg
      541 cggagcagtt ggatacatat cttacgatta tgtgcgtttt atagagaaat taccagaaat
      601 tctaccatca gattttttgt ttcctgaact ttattttcag atgtgttttg acctggttgt
      661 ttttgaccat cttaaaagaa aaattcaagt gattagcaat gcgaaaataa aaaaaggtca
      721 atcccttaaa aattcatata aaaagtcatg tgaaaaaatt gaatccatta tttcaatgat
      781 caggaagcca attccctatt tcagaaacaa cagtgctgtt gatgccggaa aatttatttc
      841 gaattataag caggatgaat ttgagtcagc agtgacgaag gcaaaaaaat atatcagggc
      901 aggtgatatt attcaggttg ttctttctca aaaatgggaa agaaacatag atgtcagccc
      961 tttgtcagta tacagaagtt tacgttcggt taatccgtct ccatacatgt tttacctgaa
     1021 ttttcctggt atttaccttg ttggttcttc cccagaaatc cttgtcagat gcaatggttc
     1081 tgatgcatta ctgcggccca tcgcaggcac cagacccagg ggaaaggatg atgcagaaga
     1141 tgaaaaactt gccgaagaat taaaaaagga tatcaaggaa attgcagaac acattatgct
     1201 tgttgacctt ggaagaaatg accttggtcg ggtgtgcagg tttggctctg ttaaagtgac
     1261 agatttaatg tttgttgaga aatattctca cgtcatgcat ctcgtgacaa atgtggtggg
     1321 aaaacttgca aaggggaaaa attattttga cctggtcagg gcatgttttc ctgcagggac
     1381 tgtcagtggg gctccaaaaa tcagggcaat ggaaattatt gaggaacttg aaaaaagtcg
     1441 tcgcagtgtt tatgctggag cagtaggata ttttggattt cattctgata tggacttctg
     1501 tatcacaatt cgtacaatgt ttggctctgc tgataaaatt tatatccagg caggtgcagg
     1561 gattgtggct gattcagtgc cagaaaagga atacattgag acaataaaca aggcaaaggc
     1621 actgatgctt gcctatgaaa atgcaaagaa gatggatgca atttaaatag gaaaaaagat
     1681 gaaggttatt ttacgtgctg gcttaatgat actgatggca gtattcgttg taaattgtca
     1741 gggaaaaaac aatgttcgca tcattgttga agcagaagac atgacgggtg ttaatcagaa
     1801 aaattttggt ccagggcaaa catggaatat tggaagatgg ggaattgacc tgtatcaaaa
     1861 catgactttt ggtggtttat gggcaagtag acttaaaaca gcgataactg atgaaaaaaa
     1921 tggtgatgca gaaatttcaa agattattga gattccgcag tcagatacat acaaggtctg
     1981 ggtgaaatac gaatgtccgc cattttttaa ttatgcgttc agggtaagaa ttattgatag
     2041 ggctggaaat actgtgtttg ataagattta tgggctgtta acttctgaaa aacactattg
     2101 ttttacagat aaggttgtca ggggttcatt gtactggcag tggggaattg accatgacgc
     2161 agcagaaggc tatgatgtaa aactggataa aggtacctat aaacttagca tttcaaaaac
     2221 tcataatcct gagccagcag gttgtcgcag tattgacgtc gtcatgatta ccaatgacct
     2281 ttccgagctt tctgctccta aatatccgag atacccttta cttgatgaat tgagaagaga
     2341 aaatcatgtg tatttcagat tcaggaacct ttcagaaaag cccatagcag tgaaatggaa
     2401 tcactggaat cacaggtatc ctgatttcta cagcccgatg tacagggatc ttgtaagatt
     2461 ttatgatgaa aacggaaagc aggtggcaaa agagggaatg aagtttacag gtgattggcc
     2521 tgagccgata caaccgggaa aggtcagtgt ctggtacgac cttggtccga caatgaatgt
     2581 ggaaagcaca tctccttata cattcaaagc aaccagtgtt gatggttcaa taaagaatcc
     2641 tcgttttgca gttgaaattg ctcttgaacc gaatgaaaaa aagattgtga aatcgtttga
     2701 gatatctcag gatgaggaag aattgaccat acttgttcag ccagatctgt acacaaaaga
     2761 aggcgttgca tttacgaaaa agattgcaga tattttcaga gacattacga tgcaattgaa
     2821 taaggaaaaa agaatagggc cgattcccaa aaaaataagg ttgtttgcat atacaggcag
     2881 ggttactgat caaccaaatt caaaatgggg atttgaaatt gaacaatcgt tcagacatgc
     2941 ccttggtatc aatacaattg gttatgtgaa tgatggtaac gaagcaaaga aagtgattga
     3001 gtggtggaaa gacaaaggtg gtgttgttga acaatcgtat tcataccatc attctcagga
     3061 tattccaaaa gtggtgaaaa tgataaagga caaacaactt gagccatact tttattatct
     3121 ttcctatggc gatgaaattg ggctgcctgt gtcaaaagca gatgacccga aattgttaga
     3181 acaattcagg gattttgtaa aaaagcatgg agagatacct gaaagtttgg gctgcgagta
     3241 ctgggaaaat gtcaaaccag tgaattctcc atcaagtgag atagcacttc aaataggagt
     3301 aatttctgat aaaaaggatg cagtatcagt atcgcaaaca atgaaaaaat tgtactggta
     3361 tagcgtgttg tttcaggaag aacttggaat aagggatttc gctgaaaaaa caaaacaatt
     3421 aaagaaggaa cttggagaac atgtccatac gtctgcaaac ctcggtggaa tgcatccttt
     3481 ttactgggtt catcagcaat cttttattga agcattcaaa catggtgcaa tgagccttgc
     3541 ctggagtgag gattacacat attgtatgcc agaggcatca gcacttgtta ttgattttct
     3601 tgcatcttat ttgagaaaag gagcaagtta tcatgacact cctatgcaat tttattgcat
     3661 gccacattat ccaggttgtc cacctgattt actcttgaaa aatgcagtac tgttgtgggc
     3721 aaataatgta aaagaccttg actttttcag cgcaggtttt gacggatata tgacagaaaa
     3781 ctatattgcc tatcgcgtgg gattacccac attcaaagcg ataaggacga tttctggcat
     3841 ggcgggtctt attgaagatt atctggtacc tgcgcgtgtt gaaaaaacac ctgttgcaat
     3901 gctgttgagc aaatcaagtg atgtatggga aattgagggg aaatctcaat gggacatcaa
     3961 accgggttct cacgcaacaa atgtgtttca ggaagaaaga aaaaatatat ggtatgcatt
     4021 gagaaaagcc ggctacaggg ttgattttat tacagaaaac gacataaaag aaggaattct
     4081 tgatagatac agagtactct acattgcatg ccagaatatt gaaaaacaac cagcagaaag
     4141 aattaatcaa tgggtaagaa atggtgggat tgtgttcgca actgctgcat cctgccgcag
     4201 ggacgagttt gaccagccat taagtactct tgatgaaacc tttggcaggg gaaaacagat
     4261 ttcttaccaa caatataaag gtccattgag agcaaaactt gaactgttat tccaattacc
     4321 ccttgactat ataatttttg acaatcaaag ttcgaaagtc ctttgcaata aagaagtatt
     4381 tgaagttgca cctgacgcag aagttatcgc aagatactgt tctgatggta tgccagcatg
     4441 gattaagaag aaaactggtt caggactcgt ttattatgga ggaacacttc ctggtcagag
     4501 ttatatccaa tatgggttaa aggtattgcc atgtggaaaa ggtggagtaa accagagttt
     4561 ttgccatttt ggagcaggga atttagaacc agtacaaaat gaccctgttt cagaagattt
     4621 aattcttctt cccctcaggg aaaacgggat attgccagat gttattacag acagaaaagg
     4681 aattgtgtca ggaagattgg catcagaaaa tgccacagtg ataccattgg taaatcttgg
     4741 tgaaagtact tccatgaaga acatttcaat caatgtgaat tctttgaatt acaatccaaa
     4801 aaaagtatgg tcgtgttttt atccaaaagg gctgaaattt tcagtgaata actctcaggt
     4861 atcaatcaaa ataccctcaa tgggtgctgc agatgtgatt gttatagagc ggtgatatgt
     4921 ctctattata taaacctgat tggcacgaga caaaggaaaa ttacaggaaa tggtgggcac
     4981 acgagtattt tggaagatgt gcgattgctg tgtatgctcc caaaaaaaat gcaccggata
     5041 tccctgcgcc accagaacca gaaagtattc atcagaagtg gtatgacctt gattacatca
     5101 gtcaggtcaa ttactacaat ctctcccgaa ctttttttgg agcagaagca ttgcctatct
     5161 ggaatggagg ttatcctggt cataccgcaa ttcccacttt tcttggttgt gaggtacatc
     5221 ttgatatgcg aactggctgg tgggaaccga ttttgaaaca ggaagatcta acatttgaac
     5281 atcttgaaat agacgaaaat catcctgatt atagattttc aattgaactt ctgaaaaggg
     5341 caattgttga agcacaaggg aaatgccttg tttcaatagg agcctttggt ggttgtggag
     5401 atacacttgc tgcattgagg ggaactgaaa gacttttgtt tgactgtatt gaaagacctg
     5461 agagagtacg agaggcagaa ttgtacttaa cggagatgtg gtgcaggcac tatgaaaaac
     5521 tttacaacat aataaaaaag gtggatgaag gttctacttg ctggtttccg ttatgggcac
     5581 ctggaaaatt ttatgcttct caaaacgatt ttgcttataa catttctcca tcaatgttca
     5641 gagaagtttt tttgcctgca ataaaaaaac aggtggaatt tcttgattac acaatttacc
     5701 atgttgatgg aataggtaat tttaaccaca ttgatgcttt gcttgaactt ccagagttac
     5761 aggcaatcca gattttgcct ggagcaggaa aaccatctcc ccttcatttt atagggatac
     5821 tcaaaaaggt acagaaggaa gggaaaaatc ttcatatcgc gattcctgca ggcgaagtga
     5881 aaactgccct tgaacatctc agtgcccgcg gactctttat ttcaacgagt tgtgaaacag
     5941 aagatgaggc attgagattg attgaaaata catacaggtg gtcagaagac cgttcttaat
     6001 ctggatttgt cataacagtt gttctcatgc ataataaata tagaggggga aaaatgcaga
     6061 caattttaat gattgataac tacgattcct ttacctataa tcttgtgcaa tatctgggtg
     6121 agctgggatg cagaataaaa gttttcagaa atgaccggat ttcaattgaa aaaataaaaa
     6181 aaatgaaatt tgacaaaatt gtaatatctc caggtcctgg cagaccagag aacgcaggaa
     6241 tatcagtcga attgattaag gtatttgaag ataaaaaacc gattcttgga gtttgtcttg
     6301 gccatcaatg tattggatat ctgtatggcg gaaaaattgt gggagcaaga tatttgatgc
     6361 atggcaaaac atctcttatt tatcacaaag acgaaaacat ttttaaaaat atttccaacc
     6421 ctttttctgc caccaggtat cattctcttg ttattgaaaa gaatagttcg ccggatgttc
     6481 ttaaaatcac agcctggagc gatgatggtg aaattatggg tatctgccat agaaaatacg
     6541 gtatttttgg tgttcagttt catcctgagt cagtcctgac agacaatggt aaaaaaattc
     6601 taaaaaattt tcttgaacta tgaacataga tgaaatattg acaaaagcag tttcgggaca
     6661 gcccattacc agaattgaat ctgctgaaat tttccgtcag attatgagtg gaaatgtttc
     6721 gcctgtgaag atggcaagtt ttcttactgc tctgagagtg tcaggggaaa caggtgaggt
     6781 tgttgcaggt gctgtggaag tgatgaggga gaaatgtttg aaggtcaaaa gtacaggaat
     6841 tgttgcagat acctgcggta ctggcggaga tcacaccggc acatttaata tttcaactgc
     6901 ttctgcaatt gttggttctg cctgtggcgt aaaggtggca aaacatggca atagagccgt
     6961 atcaagtaag agtggaagtg ctgatgttct tgaaaaactt ggtatgaata ttgagatatc
     7021 tccagaaaag gcagagatga tgctggagaa ggtgaacttt acttttcttt ttgcgcctct
     7081 gtatcatcct gccatgaaaa atgttgctgc tgtgagaaaa gaacttggat ttcgaaccat
     7141 tttcaatatc cttggaccat tgtgtaatcc agcaggagcc agtgttcaaa tactcggggt
     7201 tggcaataaa aatcttcttg acatagtacc tgacgttctt tctggtttca atgtaaaaag
     7261 ggcatggatt ttttatggcg aagacggaac agatgagatt tcaataaccg gaaaaacaac
     7321 agttattgaa attggcgaaa caagaaaaaa gttttctatt gaacctgaag agtttgagtt
     7381 aaagaaggca acattaaaag aacttgaagg cggtagtcct gaggaaaatg cggaaatatt
     7441 aaggttgatt atcagcggga agatgaaagg tgcaaaaaga gatgctgtaa tcttgaatgc
     7501 tggtgcttta ctatatcttg ctggagtagc aggcagtgtt attgacggaa tgaaaacagc
     7561 gcaggaggtt attgacagtg gatattgtac cggacacctt gaaaaaatta tttctatgtc
     7621 aaaagaaaac taaacttttc tttttttttc tcattttgcc actgggcgtt tgtgctcaac
     7681 ctgaacaggt agcaattcaa cttcttgata tcacaaatca gcgtgaacct gtgattaaca
     7741 taataaattt gaaaatagac cagttatggc actctggcag gtatgaaaag atatttccat
     7801 tgttccatct tgttgtgcga cttgaccctc tggatgaaga agcatgggca acaggaggat
     7861 ggtttttgat ttactgtgtt gctccaatga caggattccc tgagagcgaa aaaatcaaac
     7921 aaaaagggat tgaatttctt aaagaaggtt taactcataa ttttgattct tacagacttt
     7981 actgggaact tgcgtggatt tactatgcca gaggagaatt tggaaatgca attgcctatc
     8041 ttgataaggc aattcagtat gaacatcctg catatgttga aaacacaaga gcgcatgccc
     8101 ttgaaaaact tggaagaatt gatgacgcaa taaaacaatg gcaggaaata aaaaagaagt
     8161 ttcctgaaat gaggtttgtt gcagaaagat tcatcctcaa tcttcagaaa gaaaagacag
     8221 atgttaaaca aaatccttga acataaaagg aacgtggttg agcagaaaaa attgtccata
     8281 ccactggatg ttcttaaaca aagaattcag cccaccgtat gcaggtctgt gattgagaga
     8341 acaaaacaac agccatttat tattatcgct gaaacaaaaa aagcatcgcc atctggtgga
     8401 atttttagaa agaaatatag tccttctaaa atagcagcat catatgaaaa aggtggcgct
     8461 tcagcaattt ccgtgctgac agaagatcga tattttcttg gttctcctcg tcatttacaa
     8521 tctgtaaaaa atacggtgag cgtgcctgtc ttggcaaagg acttttttat tgacccatat
     8581 cagatatacg aagcaaaaat gtatggtgct gattgtattc ttttaattat caggatactt
     8641 tcggatgagc aatttatttc tttgttaagt gttgcaagaa cattaaatct tgaaattctg
     8701 gttgagttac atactgaaaa agaaatagaa aggtttttta atattatgcc ggccagcaag
     8761 gggatactgg ttggtattaa cagtcgcgac ctggacagtt tgagaataga tctgaacatt
     8821 acaatttatt tgatttcact gcttaaaggc gttaaaatac ctattattgc agaaagtggt
     8881 gttaacgaca tcaaaactgt tatagattta tacaatgctg gtgccagtgg tgcgctgata
     8941 gggagtcgtt ttttgaaaga aaaatcgcca gggataagtg taacaaaatt tttaaaggag
     9001 atttacgatg gatgaaagaa aaattctgtt gagtgagaaa gaaatgcctg tggcatggta
     9061 taatgtgctg cctgaccttc ctgaacaact tccaccacct cttgatccga ggacaggcaa
     9121 acctgttgat ccgtctgttc ttgctgcgat ttttccagtg tctttgctgc agcaggaaat
     9181 gtcagttgaa ccatggattg aaattccaga agagataaga caggtttatg caatgtggag
     9241 accaactcct ttgcacaggg cagttgctct ggaaaagtat ctgaaaacgc ctgccagaat
     9301 ttattttaag aatgaaagtg taagtccccc tggtagtcat aaaccaaata ctgctgttgc
     9361 acaggcatac tataataaga aggaaggcgt gaaaagatta tgcactgaaa caggcgcggg
     9421 acagtgggga agcgctcttt cgtttgcctg caatcttttt ggtcttgaat gtgtggtgta
     9481 tatggtaagg gtcagttatc agcaaaaacc gtatagaaga atcatgatgc aaacatggaa
     9541 tgcaaaaatt tatccgtctc caagcgacca gacaaactat ggtagaaagt tgttgaaaga
     9601 aaacccggat aatccaggaa gccttggtat cgcaatttct gaagcaattg aggatacaat
     9661 cagcggcaaa gatagtaaat acagcttggg cagtgttttg aatcatgtac ttcttcatca
     9721 aacagttgtt ggccttgaaa cacagaaaca acttgaactt atcggtgaaa aggcagatgt
     9781 tcttgtgggt tgtgtgggcg gaggtagcaa ttttgcagga ctcgttcttc catttgtgaa
     9841 ggataaaatc aaaggaaaaa gcgaggttga gattattggg gttgagccaa cagcatgtcc
     9901 gacaatgaca aagggacctt acagatatga ctatggcgac acttcaggtt taacgccgct
     9961 attaaagatg cttaccctcg gtcacgattt tatgccacct ggtattcatg ctggcgggct
    10021 cagatatcac gggcttgctc ctattgtgag tatgcttgtg gataaaaaaa ttgtcagggc
    10081 agaagcagta gctcagacaa aggcatttga agcagccgtc atttttgcca ggacagaggg
    10141 atttcttcct gcgcctgaaa catcgcatgc catcagggtt gtcattgatg aggcaaaaaa
    10201 atgcgctgaa accggtgaag aaaaatgtat tgtgtttaac ttcagcggac atggtcactt
    10261 tgacctggct tcctatgacg catatctcgc tggcaaattg caggactacg aatatcctga
    10321 agaaaaaata cgcgagtccc ttgaaaaact tcccaaaatt gaatgaaggt caaaatctgc
    10381 gggattactc aaaggaagga tgcagagcaa tgcgatgctc ttgaagtgga ttaccttggt
    10441 ttcaattttt acaggttaag taaaagatac atacttcctg aaaaagcaag agacattatc
    10501 cccttcttaa aaagggcaaa acctgtgggc gtatttgtgg aacaggattt agattacatt
    10561 aaaggaattg ttgacatgtg taatttatac gggattcaaa tacatggaag tgagaattca
    10621 gagttctgtt acagaattaa aaaacttttt ccagaccgga tggtcataca ggttttcagg
    10681 ataaaagaga caattccttc aaacttgaac gattttgatg ctgactatta tctttttgat
    10741 tcctttgatg attcaagccc tggcggtaca ggaaaaactt ttgactggac agttttacaa
    10801 aaaatggaat ctattgcgca gaggtcgttt atcgcaggtg gtataaatcc agaaaatgtg
    10861 aaagcactta tttcctgtgt agatgtgtat ggaatagatg ttgcttcagg ggttgagaaa
    10921 gtgccaggca ttaaggatat ggagaaggta agacagttgc ttgtgaaaac caaaggatgc
    10981 gtgaatgaca aaattacctg atagaagagg caggtttggt atttatggcg gaaagtttgc
    11041 gcccgaaaca ttgatggcac cgattgaaga acttgaacac gtatacttta ctactggtac
    11101 aagcaaacaa tttcgtaagg aacttgattg ttacctgaaa aattatgccg ggagaccaac
    11161 atctctttac tatgctgaaa gtttttcaaa ggagatcgga tgcagggtat atttgaaaag
    11221 agaagacctt gctttcacag gtgcccacaa gattaacaat accattgggc aggttcttct
    11281 tgctaagaaa atggggaaga cccgggtaat tgctgaaaca ggggcaggtc agcatggcgt
    11341 ggcaacagca acagcggctg cgcttttcaa acttgaatgc gaaatataca tgggtgaact
    11401 tgatatgcaa aggcaatctc ttaatgttta cagaatgcga cttctgggtg caaaggtcaa
    11461 ttgtgtgaaa tctggcagtc gtactcttaa agatgcaata aatgaagcgc tgagggactg
    11521 gatatcaaat ttcaaaacaa ctcattatgt tattggcagt gtcgtgggtt ttcatccttt
    11581 tccagtaatg gtcagggatt ttcagtcagt gatcggaaaa gaaacaaggc gtcagatttt
    11641 aagggcagaa aaacgacttc ctgattatct ggttgcg
//
LOCUS       DAORYU010000018        11458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_4324_length_11458_cov_4.951942, whole genome shotgun sequence.
ACCESSION   DAORYU010000018 DAORYU010000000
VERSION     DAORYU010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11458
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4324_length_11458_cov_4.951942"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="PLW07_01180"
     CDS             complement(<1..340)
                     /locus_tag="PLW07_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49169.1"
                     /translation="MKNKVEICRYRDIVKRICDCQPEMMVEHFYQNIGKEMFLKREKI
                     RNSIKTISQWEKERHNIRKNFQQAIGSNFPETKVSTIEKGELRKYGLTVKKIVFSLHK
                     NHWVPALIYIP"
     gene            complement(337..3312)
                     /locus_tag="PLW07_01185"
     CDS             complement(337..3312)
                     /locus_tag="PLW07_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49170.1"
                     /translation="MKIKLIMMGVFCLMFSSYLSGAKIFFEAEDMIVSETQKWQIIEH
                     FPGWYYGFPSKGKMLRGSIGGPAEARKIVSIPQQAEYRLWVRYLDVLPYRGPFKISVL
                     QQGNIKAEKTFDTESLRSTPEGKKKWGDGFGQFVWDWMDVNLEKGDAEIVISKVEVRG
                     GASWVTRHLDLLVLCDDKKYEPVMADFTPQLYIKIRMGKNHKYPCVIHTFGRRPQPPW
                     FLPHANIYKTGLVYGCYTGYKPEGANENFLRAGDETPWINTTSFFDVMGDHKLELLAM
                     QEYYNGVESSEFTVYLSNTPSDDGIFKSFSRSGKGAGMLLLIDLSRRENIISDLEGSR
                     EAFEIAKNLPEFSGKRPVKFPVMTGCAVNSSFYQPETVSNEIFILSKLGFNGIGGYDP
                     QYYEAGFTKYKGGAIYFHLAKNGCLADPNIEAITSLVKSAAENFAKQNLFKDMVAWSQ
                     MDEPGSVSMEHIVNCAVCQRSFRDYLRKMGLTPQDFGAKDFGEILPSNNPEDGKRYYY
                     TALYRNQVLADFFKIGSDILKQFVPEPKTTANFGEYLTYTGNMLHSGDDWFLILNTGG
                     LTYGWTEDWLNLGTSYQLCGYRADFLRAASKGKFGMYCIFRNPWDTQAKVASEIGHGA
                     RAIYYYNYGPYYAGIDAHSKNYKLYPATGKINHSIGTIEDYLMDAKVPQNKIALLYSH
                     TTDIWTLKQGTSVFGKERMGLWLILRHLGHPVDIITEQDVLERKINNYKGIFVVGSHV
                     PIEVMRNLVEWARNGGILYAGAGTGIYDRFNNVSDSDSYLGVKRENFYFASEPGNDYY
                     GLPGLKALDSVRFVEDTSSETIEVICGLQKMKNVSGSETMAVFSDGSPACVMKRAGKG
                     RIVISGFFPGLAYMKSAVVANRQKKQDASMSDCPPEYPGETRKMFEKIVAGIDKPVST
                     NNYLVEANLLETKDAFIITLSNWKGAPVEHLEVVLNQSAKGKPVSIENPVISYENKHR
                     QKIINVNMQSAFDFIVVPK"
     gene            complement(3312..4283)
                     /locus_tag="PLW07_01190"
     CDS             complement(3312..4283)
                     /locus_tag="PLW07_01190"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOL49171.1"
                     /translation="MFEKSTATLSASKKAVDEFVNDKLGLSVHWGIYAIIGKGEWVMH
                     VDKIDVKEYEKLMDVFNSVHFDADEWISLLKETGIKSFMITSKHHDGFCMFDTAITDY
                     KITNTPFKRDPIKELADVCHKYNVKLHFYYSLLDWHHPDYQSNWKSYVKYYQGQIKEL
                     CSNYGKIGGFIFDGWWPRHPFDESQEYFIPKGIWRIGETYDLIHSLQPDALIANNHHI
                     LPLPGEDYQVFELDLPGENTAGFNTTEIGDKPLASWINLGGGWSYIKDNRRFPEVDYF
                     LNFKKRCDERNTFLWLNAAPMPDGRLQPEQVKILKEFGEALKHEREK"
     gene            4362..5492
                     /locus_tag="PLW07_01195"
     CDS             4362..5492
                     /locus_tag="PLW07_01195"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HOL49172.1"
                     /translation="MKILICPDSFKGSLSAKEVANTICLAMSKILPDAIFITSPMADG
                     GEGTVDALLSGIGGKKYTVSVLDPLGRKINAEYAILSDGTGVMEMACASGLPLLKTHE
                     KNPLVTSTYGTGQMILEMVKKKVKHILVGVGGSATVDGGMGMAKALGVKFYSKSGKEL
                     KEGGGFLGRLAKIDVSGINQDVLKTKITVLCDVNNPLTGQYGASRIFGPQKGATEQMV
                     ELLDSNLHHYATIIKKQLKKDVEFIPGAGAAGGLAAGLYAFLNAYLVEGAKYIAEKLN
                     LEKHIKESDVIITGEGKIDSQVKFGKTILPVIETAKKYQKPVIALCGQKAKDTEFLRN
                     AGLTAIFPIVPGPVSLEEAMKNAKAYLQDTAREIACLIKCFS"
     gene            5480..8455
                     /gene="mfd"
                     /locus_tag="PLW07_01200"
     CDS             5480..8455
                     /gene="mfd"
                     /locus_tag="PLW07_01200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOL49173.1"
                     /translation="MFLLNQLKALKSVDIWQETFHKIKNPQTLHICGVNPGFLSFLIS
                     GFYVAQPDRIFLLVCQDPEIAETFYANIQSFLPDSVFLFPEKDVHLPPDHPIQEKEIK
                     RVKTLAGIDEKKTGVVVTSIQAISVLLPSVTDFLSHIIEIKVGIEIPRSKLIARLVAY
                     GYEEVSVVENPGEFCRRGGIIDFFPFGENPVRIEFSGDTVQSLRTFDVQTQMSTGKIN
                     VCTLLSLNESFISIENNSTIFDYLNTNTLPFVYGARAFEEKYHKLCELSHRSIKKDIA
                     DWQRLNKHFSNAIHLVMEIPAETDIDKIIMFPVDNPTRFGFIKEQFVWNPVKDEKVFI
                     VEDNKTHRENLKKILAEHKVIIDGHQFLDGYLSHSFTYPGINLTLLNTATLFNVKPGR
                     AMRQFMEAMPVSQREELEKGDYVVHYQHGIAKFDGFEKIGHEDEEFIKLEFADGEKLY
                     LPLSDIDFIHKYIGTKEHPVLSKLGSKTWIRIRDSVKEGIRNIAEQLYTLYIERKRGT
                     GIAYPVDDEFQRMLEESFPYEETPDQIKALQEIKNDLESTKIMDRLLCGDSGYGKTEL
                     AVRAAFKVVSGGKQVAVLCPTTVLSQQHLRTFRQRMQSFPVTIEMLSRLYSDSKQKQI
                     LENLAKGNIDIIIGTHRILQDDVIFKNLGLLVVDEEQRFGVIHKEKLKTKFRNVEVLT
                     LTATPIPRTLYLSLSGLRDISLLETPPSGRLSVATYIGRYNDTIVRMAIFNELARQGQ
                     VFYLHNRIYDITKVKNKIQQMFPATSIAISHGRMKEHELASVMEDFADGKIQILVATT
                     IVENGLDVPNANTLIVDDAHRYGLADLYQLRGRVGRYKVKAYAYFLIPQHLQVAGQVK
                     ERLKALGELVKPGSGMKIALRDLQIRGAGDILGKKQSGYINQVGFQLYCRFWKEISSK
                     FTGETVSEPEAKPVMRGFIDPEWIPSPGLRFELYRKISEIQNTQQAKKILAEMKDRFG
                     EVPERVKKLIFSQVTKNFD"
     gene            8515..9471
                     /locus_tag="PLW07_01205"
     CDS             8515..9471
                     /locus_tag="PLW07_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49174.1"
                     /translation="MPVKFDFSPAEFLPFKDKKVIEKVRKIKKQDICKHPNKNFKIRV
                     IEDQTAFVFAWTLHIVGGIKKALEEGKQHVIILPAPNPQYALIAEMINQLDISCKHVH
                     TFNMDEYADENGNTAPREWKGGFQYWMWHDFFNRIKPELRMPEKQIHFPSTKNVNYYS
                     KMMEDLGGADICYGGIGWCGHIAFFEPHLGIDYVNDIEGYLQLGSRIVDLHPITLCQN
                     SLFADAGCSGDIAACPPKAATIGPRDLKNSKLVSFWDGFSYGDVSWQRFISRLAAHGP
                     VTPLIPASILQILNSELTLSGSVAEDCKTETGERRVPIQFPE"
     gene            9474..10481
                     /locus_tag="PLW07_01210"
     CDS             9474..10481
                     /locus_tag="PLW07_01210"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOL49175.1"
                     /translation="MDINLKGKIIDIHSHAGVSIKAYMGQEYPYAATIEGLAFRQERY
                     GVDVNVVFPLSADLFFDLHYLKKGICKNAKKTLSSVPYEIENALLLKEVYAFCPEYEG
                     RFIPFISVDPGRMVKKQVKNILDLEKKYRIYGIKIVPVVCQSSILRLLDEGKAFLEIA
                     RERNWPFLIHTTTHKEENFSHARLVFRVIEKNPDIRFCLAHCIGFDRNYLNLAHSLEH
                     VWVDTSALTIQVQLARENHPIAAAREDRFDADYNDHKKVLRTLIEAFPETIIWGTDTP
                     AYSYIARRKQGTGKNSYVETWLKATYKDEIDALRSLPEKSMMQIANRNSINFLSGSHE
                     N"
     gene            10471..>11458
                     /gene="larA"
                     /locus_tag="PLW07_01215"
     CDS             10471..>11458
                     /gene="larA"
                     /locus_tag="PLW07_01215"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HOL49176.1"
                     /translation="MRIKLRYGKKGLPLDFDGNITVFEGKNILPVEKPQEKISQCLKN
                     PVGCKGLAEIVRDKKPGSVCIVISDHTRAVPNKIILPAIINELLLAEVPEKNIFILIG
                     NGTHRPTTTQEKIELVGKEIFEKFPVYDHSATDTSMLVHFPGTDFYVNKRYVESDLKI
                     VTGMIEPHFFAGFSGGRKGVCPGIAGMESIKIFHGAKFLEDEHTKPGILKNNLCHRFA
                     TEFAKKAGVDFLVNVTIDKEGSITGIFSGELESAFEKGVEFCRSMSTAYAEKKYPIVI
                     TTNGGYPLDRDFYQSVKGMVTALDIVEEGSIIISASECSDGVGNEGFRKLLSE"
BASE COUNT         3667 a         2250 c         2146 g         3395 t
ORIGIN      
        1 ctgggatgta aatcaaagca ggtacccagt ggtttttatg aagagaaaac acaatctttt
       61 tcactgtaag accatatttt ctaagttctc ctttttcgat agtgctaact ttggtttctg
      121 gaaaatttga accaattgcc tgctgaaaat tttttcttat gttgtgtctt tctttttccc
      181 attgagaaat tgttttaatg gaatttctta tcttttctct tttgagaaac atttcttttc
      241 cgatattctg gtagaaatgt tcaaccatca tctctggctg gcaatcacaa atccttttca
      301 caatgtccct gtatctgcaa atttcaacct tattcttcat tttggtacca ctataaaatc
      361 aaatgctgat tgcatattta cgttgatgat tttttgcctg tgtttgtttt cataagaaat
      421 tactgggttt tctatggaaa ctggttttcc ttttgcgctc tggtttaaca caacctccag
      481 atgttccaca ggtgcacctt tccagtttga tagcgtaata atgaaagcgt ccttagtttc
      541 aagaagattt gcctcaacaa gataattgtt cgttgaaaca ggtttatcaa tccctgccac
      601 aattttttca aacatttttc ttgtctcgcc aggatattct ggtggacaat cagacattga
      661 agcatcctgt ttcttctgcc tgtttgcaac aactgctgac ttcatatacg caagtccagg
      721 gaaaaacccg ctgataacaa tccttccttt tccagccctt ttcattacac aagcaggcga
      781 gccatcagaa aacactgcca ttgtttcaga accacttaca tttttcatct tctgcaatcc
      841 acagatgacc tctattgtct cagaagatgt atcctcaaca aatcttactg aatccagtgc
      901 ctttaatcca ggcaaaccgt agtaatcatt tcctggttct gaagcaaagt aaaagttttc
      961 tcttttcact ccaaggtacg aatcactatc agaaacattg ttaaacctat cataaatacc
     1021 agtccctgca ccagcataaa gaatgccacc atttcttgcc cattcaacca gatttctcat
     1081 cacctcaatc ggtacatgtg agccgacaac aaaaattcct ttataattat tgatttttct
     1141 ttctaacaca tcctgttcag taataatgtc aacagggtgt ccaagatgcc tcaaaatcag
     1201 ccagagtccc attctttctt taccaaaaac tgaagtaccc tgttttaaag tccagatgtc
     1261 tgtggtatgt gaatacaaaa gcgcaatttt gttttgtggc acttttgcat ccataaggta
     1321 atcttctatg gtaccaattg aatgattaat tttccctgtg gcaggataca gtttgtaatt
     1381 ttttgaatga gcatctatac ccgcataata gggaccataa ttatagtagt aaattgccct
     1441 tgcgccatgg ccaatttcag atgccacctt tgcctgagta tcccagggat ttctgaaaat
     1501 acagtacatc ccaaatttcc cttttgaagc agccctgaga aaatccgcac gataaccgca
     1561 taactgataa cttgtaccaa gatttaacca gtcctcagtc catccgtatg tcagtccccc
     1621 tgtattaaga atcaaaaacc agtcgtcgcc tgaatgcaac atgttccctg tataggtcaa
     1681 atattcacca aaattcgcgg tagtttttgg ttcaggaaca aattgtttca gtatgtctga
     1741 gccaattttg aaaaagtcag ccagcacctg atttctgtaa agcgcagtgt agtaatacct
     1801 ttttccatct tcagggttgt ttgaaggaag tatttcacca aagtcctttg ctccaaaatc
     1861 ctgtggcgta aggcccattt tcctgagata atccctgaaa cttctctgac acactgcaca
     1921 attgacgatg tgctccatac tcacagaacc tggttcatcc atctgactcc aggcaaccat
     1981 atccttgaac aaattttgtt ttgcaaaatt ttctgctgca gattttacaa gggatgttat
     2041 tgcctcaata ttggggtctg ccaggcatcc attttttgca agatggaaat aaatcgctcc
     2101 acccttatat tttgtaaatc ctgcttcata atactgtggg tcataaccac caattccatt
     2161 gaacccgagt tttgaaagaa taaaaatttc attgctgact gtttctggtt gataaaatga
     2221 tgagttgact gcacatcctg tcatcacagg aaattttaca ggtcttttcc ctgaaaactc
     2281 tggtaaattt tttgcaattt caaatgcttc tctgctaccc tcaaggtcac tgatgatatt
     2341 ttcacgcctt gataggtcta tcaaaagtag cattcctgcg cctttgcctg aacgactgaa
     2401 tgacttaaaa attccgtcgt cagatggagt atttgaaagg tacactgtaa attctgatga
     2461 ttctacccca ttgtaatact cctgcatagc aagtaattcc agtttgtggt ctcccatcac
     2521 atcaaaaaat gaagtagtat taatccatgg tgtttcatcg cctgctctta aaaaattctc
     2581 gttggcacct tctggtttat atccagtata gcaaccatac acaagcccgg ttttatagat
     2641 attggcgtgt ggaagaaacc atggtggttg aggtcttcta ccaaaggtat ggatcacgca
     2701 cggatattta tgatttttcc ccatacgaat tttgatgtac aactgtggtg tgaaatcagc
     2761 catcactggc tcatattttt tatcatcaca tagcacaagc agatcaagat gtcttgtcac
     2821 ccagcttgct ccaccacgga cttctacctt tgaaataaca atttctgcat cacccttttc
     2881 cagatttaca tccatccagt cccacacaaa ctgtccaaaa ccatcacccc actttttctt
     2941 tccttcaggc gtacttctta aactttctgt gtcaaaggtt ttttctgcct tgatattacc
     3001 ctgctgtaat actgaaatct taaaaggacc cctgtatgga agaacatcaa gatatctcac
     3061 ccataatctg tattcagcct gctgaggaat gcttactatc tttctcgctt cagcaggacc
     3121 cccgattgaa ccacgaagca tctttccttt tgaaggaaac ccataatacc agccaggaaa
     3181 atgttcaata atctgccact tctgtgtttc actcacaatc atgtcttctg cctcaaaaaa
     3241 tatctttgct ccagaaagat aactggaaaa catcaagcag aatacaccca tcattatcaa
     3301 ttttattttc attatttttc cctctcgtgt tttagtgctt ctccaaattc cttaagtatt
     3361 ttcacctgct ctggttgcaa tcttccatca ggcatcggtg cagcattcag ccataaaaat
     3421 gtgtttcttt catcacatct tttcttaaaa ttcaaaaagt aatctacctc tggaaatctc
     3481 ctgttatcct tgatgtatga ccagcctccg ccaagattaa tccaagaagc aagcggttta
     3541 tctccaattt cagtcgtatt aaatccagca gtattttctc caggcaaatc aagttcaaac
     3601 acctgataat cttctccagg aagtggaagt atatgatgat tatttgctat cagggcatct
     3661 ggctgaagag aatgtattaa atcgtatgtt tcgccaattc tccatatccc tttcggaata
     3721 aaatactcct gactttcatc aaacggatgt cttggccacc acccatcaaa aatgaaacca
     3781 ccaattttgc catagtttga gcataactct ttaatctgtc cctgataata cttcacatag
     3841 gacttccagt ttgattgata atcaggatgg tgccagtcaa gtaaagaata ataaaaatgc
     3901 aatttcacat tatatttgtg acagacatct gccagttcct tgatagggtc tcttttaaat
     3961 ggtgtatttg taattttgta atcagttatt gctgtatcaa acatgcagaa accatcatga
     4021 tgttttgatg taatcataaa tgacttaatc cctgtttcct tcaataagga aatccattca
     4081 tcagcatcaa aatgtactga attaaacaca tccatcaatt tctcatattc cttcacatct
     4141 attttatcaa catgcatcac ccattctccc tttccgatga ttgcataaat tccccagtga
     4201 acagaaagcc cgagtttatc attgacaaat tcatcaactg cctttttact tgcgcttaat
     4261 gttgctgtac ttttttcaaa catatcccct cctggcagtt ttgttgttaa taatgttaaa
     4321 tcaaattaaa gaaaaaagaa actcagggta taatatcaat tatgaaaatc ctgatatgtc
     4381 ctgactcatt taaggggtct ctcagtgcaa aagaggttgc aaatacaatc tgcctggcaa
     4441 tgagtaaaat tttgccggat gcaatattta ttacttcacc gatggcagat ggtggtgaag
     4501 gaactgtgga tgctctttta tctggcattg gcgggaaaaa atatacagta tctgtgcttg
     4561 accctcttgg aagaaaaatc aacgccgaat atgcaattct ttctgatggc acaggcgtaa
     4621 tggaaatggc ttgtgccagc ggactgcccc ttttgaaaac acatgaaaaa aatccattgg
     4681 tcacatcaac atatggcaca ggccaaatga ttcttgaaat ggtaaaaaag aaagtaaaac
     4741 atattcttgt gggcgtgggc ggaagtgcca ctgttgatgg tggaatggga atggcaaagg
     4801 cacttggagt aaagttttat tcaaaatcag gaaaagaatt aaaggaagga gggggattcc
     4861 ttggacgact tgcaaaaata gatgtttctg gtatcaacca ggatgttttg aaaacaaaaa
     4921 ttactgtgct ttgtgatgtg aacaatccat taacaggaca atatggtgca agtagaatat
     4981 ttggtcccca aaagggagca acagaacaga tggtggaatt gcttgatagc aaccttcatc
     5041 attatgccac tatcataaaa aagcaactca aaaaggatgt tgaatttatt cctggagcag
     5101 gcgctgctgg tggacttgct gcagggcttt atgccttttt gaatgcatat cttgttgagg
     5161 gtgcaaaata tattgctgaa aaactaaatc ttgaaaaaca cattaaggaa tcagacgtca
     5221 ttatcacagg tgaagggaaa attgatagcc aggtaaaatt cgggaaaacc atcctacctg
     5281 tgattgaaac agcaaaaaaa taccagaaac cagtcattgc gttatgtggt cagaaggcaa
     5341 aggatactga atttctacgc aatgctggat taacagcgat attcccaata gtacctggtc
     5401 ctgtatcact tgaagaagca atgaaaaacg caaaggcata tttacaggat acagcaaggg
     5461 aaatcgcctg tttaataaaa tgtttctcct gaatcaactt aaagcactga agtcagtaga
     5521 tatctggcag gaaacatttc ataaaattaa aaatccgcaa acactccata tttgcggagt
     5581 aaatcctggc tttctttcat ttcttatttc aggtttttat gttgctcaac ctgacagaat
     5641 ttttttactg gtctgtcagg accctgaaat tgcagaaaca ttttatgcaa atatccagtc
     5701 gtttctacct gattctgtat ttttatttcc agaaaaagat gtacatcttc caccagacca
     5761 tcctatccag gaaaaagaga taaaacgagt aaaaacactt gctgggattg atgaaaaaaa
     5821 aactggtgtt gtggttactt caattcaggc aatttcggtg ttactaccca gtgtcactga
     5881 ttttctctct catatcattg aaataaaagt cggcattgaa atacccaggt caaaactcat
     5941 tgcgcgattg gtcgcatatg gatatgagga ggtctctgtt gttgaaaatc caggtgaatt
     6001 ttgcagaagg ggtggtatta ttgacttttt tccgttcgga gagaatcctg tcaggattga
     6061 attttctgga gatactgtac agtccttaag aacgtttgat gtacaaaccc agatgtcaac
     6121 tggaaaaatc aatgtatgca cacttctttc attaaacgaa agttttatca gtattgaaaa
     6181 taattctact atctttgatt atctaaacac aaacactttg ccctttgtat atggtgcaag
     6241 ggcttttgaa gaaaaatatc acaaactttg tgaactatcc catcgttcaa taaaaaaaga
     6301 tatcgctgac tggcaaagat tgaacaaaca tttctctaat gcaatccatc ttgtaatgga
     6361 aatcccggct gaaacagaca ttgataaaat cattatgttt cctgttgata atcccacgag
     6421 gtttggtttc atcaaggaac agtttgtctg gaatccagtg aaggatgaaa aagtattcat
     6481 tgtggaagac aataaaacac acagggaaaa tttgaaaaaa atccttgccg agcacaaggt
     6541 aataatagat ggccatcaat ttctggatgg ttatctttcc cattctttta cgtatcctgg
     6601 tatcaattta acgcttctca acacagccac gcttttcaac gtaaaacctg gcagagccat
     6661 gcgtcagttt atggaagcaa tgcctgtgag tcaaagagaa gaacttgaaa aaggagatta
     6721 tgttgttcac tatcagcatg gcatcgcaaa atttgatgga tttgaaaaaa tcgggcatga
     6781 agacgaggaa tttataaaac ttgaatttgc tgatggagaa aaactgtatc taccactgag
     6841 tgatattgat ttcatccata aatacatagg aacaaaagaa catccagttc tcagcaaact
     6901 gggtagcaaa acctggatac ggatacgaga tagcgttaaa gaaggaataa gaaatatcgc
     6961 agagcaactt tatactcttt atattgaaag aaaacgtggc acaggcattg catatcctgt
     7021 ggacgatgaa tttcaaagga tgcttgaaga aagttttcca tacgaagaaa ccccagacca
     7081 gatcaaggca ctgcaagaaa ttaaaaacga ccttgaaagc acaaaaatta tggatagatt
     7141 attatgcgga gactcaggat atggaaagac agaacttgca gtgcgtgctg ccttcaaggt
     7201 tgtttctggc ggaaaacagg ttgcagtatt atgtcccact actgtgctgt cacagcagca
     7261 tctgcgaaca ttcaggcaga ggatgcaatc ctttccggtt accattgaaa tgctttcacg
     7321 attgtattca gattcaaagc aaaaacaaat tcttgaaaat cttgcaaaag gaaacataga
     7381 cattattatc ggaactcaca gaatcttgca ggacgatgtg atattcaaaa atcttggctt
     7441 acttgtagtc gatgaagaac aaagatttgg tgtgattcat aaagaaaaac tcaaaacaaa
     7501 attcagaaat gttgaggttt taacacttac agcgacgcct attccgagaa ccctgtatct
     7561 ttcactttct ggattaaggg atatcagcct tcttgaaaca ccgcccagtg gaagattatc
     7621 agttgccaca tatattggca gatacaatga taccattgta agaatggcta tcttcaatga
     7681 acttgcaaga cagggacagg ttttttatct tcacaacaga atctatgata ttacaaaggt
     7741 aaaaaataaa attcaacaga tgtttcctgc cacaagtatt gcgatttctc atggaagaat
     7801 gaaggaacat gaacttgcct ctgtaatgga agattttgca gacggaaaaa tccagattct
     7861 tgttgcaaca accattgtcg aaaacggact tgatgtaccc aatgcaaata cattaatcgt
     7921 tgatgatgcc catcgctatg gactggcaga cctctatcaa ctcaggggaa gagttggaag
     7981 atataaagta aaagcatatg catacttttt aattccacag catctgcagg ttgcaggaca
     8041 ggtgaaagaa agattaaagg cactgggaga acttgtcaaa ccaggtagtg gtatgaaaat
     8101 tgcgttaaga gaccttcaaa tcagaggtgc tggcgatata cttggtaaaa aacaaagtgg
     8161 gtacatcaat caggtaggat ttcaactcta ttgcagattc tggaaagaaa tatcttcaaa
     8221 attcactggc gaaacagtgt ctgaaccaga agcaaaacca gtgatgcggg gttttattga
     8281 ccctgaatgg ataccttccc ctggcttaag atttgagtta tacaggaaaa tctcagaaat
     8341 acagaatacc cagcaggcaa aaaaaatcct tgcagaaatg aaagacagat ttggtgaggt
     8401 ccctgaaagg gtaaaaaaac ttatcttttc tcaggtgact aaaaattttg actgaaaaat
     8461 caaaatatgt tttattaaac acagccatgt aatcttaacc atggaggaaa aatcatgcca
     8521 gtaaaattcg atttcagtcc agccgagttc ttaccattca aagacaaaaa ggtgattgaa
     8581 aaggtaagaa aaataaaaaa acaggatatc tgcaagcatc cgaacaagaa cttcaaaatt
     8641 agagtaatag aagaccagac agcctttgta tttgcatgga cacttcatat tgtgggtggt
     8701 ataaaaaaag cccttgagga ggggaaacaa catgttatca ttcttcctgc gccaaatccc
     8761 cagtatgcgc ttatcgctga aatgattaat caattagata tttcctgcaa acatgtacat
     8821 accttcaaca tggatgagta cgcagatgaa aatggaaaca cagcaccaag agaatggaaa
     8881 ggtggttttc aatactggat gtggcatgat ttcttcaatc gcataaaacc tgaattaagg
     8941 atgccagaaa aacagataca tttcccgagc acaaagaacg tcaattatta cagcaagatg
     9001 atggaagacc ttggtggagc tgatatatgc tatggtggaa ttggatggtg cggccacatc
     9061 gcgttttttg aaccacatct tggcattgat tatgttaacg acattgaagg ttacctccag
     9121 cttggctcaa gaatcgttga ccttcatccc atcacattat gtcagaatag cctttttgca
     9181 gatgctggct gttcaggaga tattgcagca tgtccaccaa aggcagccac gattggacca
     9241 agggatctga agaattctaa actggtgagt ttctgggatg gtttttccta tggagatgtt
     9301 tcctggcaga gatttatttc acggcttgca gcgcatgggc ctgttactcc tcttattcca
     9361 gcaagcatcc ttcagatact caatagcgaa ctaacacttt caggcagtgt tgctgaagat
     9421 tgtaaaactg aaacaggcga aagacgcgtt ccaatacaat ttccagaata gccatggata
     9481 tcaatcttaa aggaaaaatt attgacattc attcacatgc tggagtttct attaaggcat
     9541 atatgggtca ggaataccct tatgcagcga ccattgaagg actggcattc agacaggaaa
     9601 gatatggagt tgatgtcaat gtcgtgtttc cattgtctgc tgatttattt tttgacctgc
     9661 attacttaaa aaagggcata tgtaaaaacg caaaaaagac cttgtcatct gttccatacg
     9721 aaattgaaaa cgctctcttg ctgaaggaag tgtatgcttt ctgtcctgag tatgaaggaa
     9781 ggttcattcc atttatttct gttgaccctg gcagaatggt aaaaaaacag gtaaaaaata
     9841 tcctggacct tgaaaagaaa tacagaattt acgggataaa aattgttcct gtagtatgtc
     9901 agtcaagtat tcttcgcctg cttgatgaag gcaaggcatt tcttgaaatt gcaagagaaa
     9961 gaaactggcc atttctcatt catacgacta cccacaaaga agaaaatttt tcacatgccc
    10021 ggcttgtttt cagggtaata gaaaaaaacc ctgatatacg attctgcctc gctcattgta
    10081 ttggctttga tagaaactat ctcaatcttg cacatagttt ggaacatgtc tgggttgaca
    10141 cttctgcatt aacaattcag gttcaacttg caagagaaaa ccatcccatt gcagcagcca
    10201 gagaagacag gtttgatgct gattataatg accataaaaa ggtattaagg actcttatag
    10261 aggcatttcc tgaaactatt atctggggca cagatacccc tgcttattca tacattgcaa
    10321 gaagaaaaca gggaacagga aaaaattcct atgttgaaac atggctgaaa gcaacttaca
    10381 aagacgaaat agacgcctta agaagtttac ctgaaaaatc aatgatgcag attgcaaata
    10441 gaaacagtat taactttctt tctggcagtc atgagaatta aattgaggta tggaaaaaag
    10501 gggctaccac ttgactttga tggaaacatc acagtattcg aaggaaaaaa tattttgcca
    10561 gttgaaaaac cccaggaaaa aatatcacaa tgtttaaaaa atccagttgg ctgcaaaggt
    10621 cttgctgaaa ttgtacgaga taaaaaacca ggtagtgttt gtattgtcat atcagaccac
    10681 acaagggctg tcccgaataa aattatcctg ccagcaatta ttaatgaact acttttagca
    10741 gaagtgcctg aaaaaaatat tttcattctg atcggaaatg gaacacacag accaacaacc
    10801 acccaggaaa aaattgaact tgtgggtaaa gaaatttttg aaaaatttcc tgtgtatgac
    10861 cacagtgcaa ccgacacatc aatgcttgtc cattttcctg gcactgattt ttatgtcaac
    10921 aaacgatatg ttgaatctga cctgaaaatt gtaacaggaa tgattgaacc acattttttt
    10981 gctggttttt caggtggaag aaaaggtgtt tgccctggca ttgctggcat ggaatcaata
    11041 aaaatatttc acggtgcaaa gtttcttgaa gatgaacata caaaaccagg aattcttaaa
    11101 aataatctgt gccatcgttt tgccactgaa tttgcgaaaa aagcaggtgt tgactttctt
    11161 gttaatgtga ccattgataa agagggaagt atcacaggaa ttttttcggg agaacttgaa
    11221 agtgcttttg aaaaaggtgt tgaattctgt agaagcatga gcactgcgta tgctgagaaa
    11281 aaatatccaa ttgtcattac aacaaacggc ggctatcctc ttgacagaga tttttatcag
    11341 tcagtgaaag gaatggtcac tgcgcttgat atcgttgaag aaggtagtat tatcatcagt
    11401 gcatcagaat gcagcgatgg agttggaaat gaaggtttca gaaaattgct ttcagaat
//
LOCUS       DAORYU010000019        11334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_4374_length_11334_cov_4.083961, whole genome shotgun sequence.
ACCESSION   DAORYU010000019 DAORYU010000000
VERSION     DAORYU010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11334
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4374_length_11334_cov_4.083961"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="PLW07_01220"
     CDS             complement(<1..534)
                     /locus_tag="PLW07_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49177.1"
                     /translation="MKIVEVETKSGTNKILQKAIDDVYQSGGGTVRICSGTFHMYDSL
                     HLRSGVNVVGVKGKTILKKCRSQSSKLSADLGYGHYDVSVEKPGLFKPGMGVYIRDVN
                     GCGFYGTVATVTWKDGDRIGINRMLNHDYARIRKAMVSTVFPVISGYYANNLVIEGIT
                     IDGNAEENEFIDGCRGGG"
     gene            complement(531..1241)
                     /locus_tag="PLW07_01225"
     CDS             complement(531..1241)
                     /locus_tag="PLW07_01225"
                     /inference="COORDINATES: protein motif:HMM:NF016269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HOL49178.1"
                     /translation="MKTKVAGLCLMVFLFWSGCVYAAPKSLVKRIAEAKEFLDDIMEA
                     PDTEIPAALFEKCRGIIIMKQYKAGFIFGIKGGYGIAMAKDEKTGEWSPPAFVKSGEG
                     SYGVQIGGQAVDSVLLIMNKDGMEMLMKTKFKLGVDGSVAAGPVGRDASAKLGPGTAV
                     LVYSRARGLYAGATIEGGLIINDDKANEQFYGIKGITVQDILFTNTVEMPDSARPLVD
                     ALKKYGMKYNEIKENKTR"
     gene            complement(1255..1791)
                     /locus_tag="PLW07_01230"
     CDS             complement(1255..1791)
                     /locus_tag="PLW07_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOL49179.1"
                     /translation="MNFLKLVQIRQSVREYRTDSVPQEVLDRCFEAARIAPSACNSQP
                     WSFIAITDKELINQMAERAFSGIYGLNSFAKNAPVIIVVITERSRYSACLGGYFRGTQ
                     YNLIDIGIAVEHFILQATEEGLGTCWIGWFNEREIHKILNIPLRRKIDVLVTVGYPAD
                     SEVREKKRKSLEEIRKYR"
     gene            1898..4639
                     /locus_tag="PLW07_01235"
     CDS             1898..4639
                     /locus_tag="PLW07_01235"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOL49180.1"
                     /translation="MTNYLFDKSYDIVVVGGGLSGIGYAIAAASGKKVLVIERRSSLG
                     WEVSTAFSSEFNGKLSKTGKILKKELESRHGIKKDKVSPALFEISLESILKKMNIDIM
                     FYTYPLDIIKRDDLIAGLIAGNKNGAGIISSRIFIDATENFSLLKKCSDRFMADKNYS
                     HIYYIFFNGVKKEIKDQEIVNRKINDIEILSIKKGFRKGEVFAEIETRNKNITETRIQ
                     IPTLISFLKENIDFLKESLVSHISNEPVCSKPQISCKFSSLMRNLYPVIYSDDDKQNS
                     SLVVQKFEAGTKTADSLKNIDEIFPDDISVFECERYTNCDEKFEFDVVVAGGGTAGAV
                     AAISSAREGIKTAVVEHSNCLGGIGTGGGIHHYYFGMGGGLQKEIEEKSSELTKLFTG
                     KFKIHGWHPEAKKIVLEEMCKNAGVKVFYGNCCIDVEMKVNAISGIYCATADGMKKYK
                     ANVVIDATGDGDIAAKAGAPFTLGRVADKLPQPFTLVPVVLTPDGTFAYINFDSGFVD
                     PTDTLDLTRARRQSLMLFEKEITNELDIPLYICPIIGIRQGRQILGKYVLTLTDQIFG
                     ARFSDAVCSMDSHYDNHSKDMENQSFQSLLWRWLTDNFMTRIECEIPYRCLLPLNVKG
                     LIVTGRAISMDPEAHYALRMEKDMERLGEVAGIAASISVKNGVFPEDIDIKKLQEKLK
                     ASGCWNPDYMIKSAININSSFEDLKQKFLSKDFKEPVSIWTFNFSEKVEKFLNAILKK
                     PDDTQLQFWSTTILSIHKKKSGVKFLIEWLKENKDINSQLCISIITILGRTKDSMALD
                     LLHGILFDKNANADTLIATIRAIGEMRNEKSIAAIDKFLKRKDIPNERILSQGVPGLI
                     KKVKENALWQIELACAETLKKLGNFRNEIVDKYLSDTRAYVRNYAKMIMDKQGVAYG"
     gene            4632..5693
                     /locus_tag="PLW07_01240"
     CDS             4632..5693
                     /locus_tag="PLW07_01240"
                     /inference="COORDINATES: protein motif:HMM:NF024126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOL49181.1"
                     /translation="MVKNHDGCYTVAGYLAELFDSEKRILELKANNRVEFLQWKSKAK
                     KKLKELIGYYCFKRCNPSPKINETVECDGFTRIHMEIYTEPSIRMPFYVLKPKDERQR
                     YPVVMALHGHVSGGKYAVVGRYDIPELVEWIKLCNYDYGVQFVKQGFLVFCPDARGFG
                     ERQEQWAKGNITSPSCFWLNNMAIPLGMTVTGMWTWEVHRLIDYIKTRNDVEKDTIAC
                     VGLSGGGLQTLWASALDERIKCCVISGYMYGYKESLLEMCTNCSCNYVPNLYRYFDMG
                     DIAALIAPRAMLVETGTKDALNGKSGLKNVFSQTAKIKKAYKIFNKEEFFKHHVFDGH
                     HRWNGEEAIPWVKKHLLGQ"
     gene            5663..6970
                     /locus_tag="PLW07_01245"
     CDS             5663..6970
                     /locus_tag="PLW07_01245"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOL49182.1"
                     /translation="MGEKTSTGTMNSEKKILSTGIIGMGGFAAAHHRAINELEKKGMV
                     KLCCTCDIAPENFKQMIENLCFEKRGVEIFDNYLKMLDGCEEKLDFVTIPTPIPLHAE
                     MHQQCVKKQIPVYLEKPPTLDYQQFISMMEVEQYATKQTNVGFNFIIQKLRHRIKQRI
                     LSNEFGNIKKITFMGQWPRPSSYFRRSSWAGKLKLNNDFVLDSCFGNAISHYVHNILF
                     WCGKGDIFSWDPVEEVHAELYRTHDIQGTDTVFAIASTRHVNNIRIALTHACAEKTRD
                     IEKIYCENAEIHFEGNATGNNGYCAQCTIYWNNRQKEMIEEPYENLLRDNIEYYARYL
                     LGEHQRPLILLSDTEPFVLFNGLIYVAAKKITQIPEQSIERINLDNGDYFLSIKNIKE
                     IAQKFIETGKFPSEQNIQWARSGGHAKCDQIKDLHRIIWNIIS"
     gene            complement(6972..7145)
                     /locus_tag="PLW07_01250"
     CDS             complement(6972..7145)
                     /locus_tag="PLW07_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49183.1"
                     /translation="MTKKFGKIEMTVWKFIRLVVFLIVTIVGFVLAHYNPQFSTFGWG
                     YTFGFIGMAILIG"
     gene            7237..8079
                     /locus_tag="PLW07_01255"
     CDS             7237..8079
                     /locus_tag="PLW07_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49184.1"
                     /translation="MNPGIFHYSKSNNFKVRQINKAKQYDVLEVTFQSSQPTGIDVCD
                     KVSGFYYRVPDARRAVIILHGLSMISVTKFLCLHLARNGFSSFLIIMPYAPKRIPRKR
                     PIRHLPQNQDWVEIFRRGLIQAVIDVRKTIDFLEKENEKIGILGISLGASIANLVHSI
                     DSRISSGIYVVGGGDLAGMLWDSRDFMARIYKRKLLGSIDKLTLMTRWKDIDPLTYAR
                     KLENVLMINARYDTSVKPVYTLKLWKSVGKPEIYWLRCAHFFLSHIFFVKRLILKHFR
                     KTLS"
     gene            complement(8074..8847)
                     /locus_tag="PLW07_01260"
     CDS             complement(8074..8847)
                     /locus_tag="PLW07_01260"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOL49185.1"
                     /translation="MKQTMAFNLQGKKALVTGGNSGIGFGIASALVKAGATVVIVGKN
                     DKKNNIALCKLKKICDRCDSVRFDLVNYKKIPVFYKNLQRKYDVFDILVNCAGITVRK
                     RADLLSIKEWEKVIAVNLTSAFVLTAQWAKSLINKNSGGCCVMVLSLMSEVARPTTCA
                     YGASKAALKQLVKSFAVDWAKYGIRVNGISPGYIKTELTEPLYNDSKFSDWVVKRTPL
                     GRWGIPDDIGPAVIFLCSDEASFITGQTIIIDGGFLASL"
     gene            complement(8844..9248)
                     /locus_tag="PLW07_01265"
     CDS             complement(8844..9248)
                     /locus_tag="PLW07_01265"
                     /inference="COORDINATES: protein motif:HMM:NF020950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HOL49186.1"
                     /translation="MKTFSALCIAGILIMSFVAGCIQKEIAFSVSLLEENQMETELVV
                     KSGDTFRIELKSNPTTGYEWFAKYDEMMLNLKRSEYISPQSQKMVGSPGKQVFIFQAL
                     KKGETIVEFTYQRSWEKDKPPVKIMKCKVMSK"
     gene            9323..9395
                     /locus_tag="PLW07_01270"
     tRNA            9323..9395
                     /locus_tag="PLW07_01270"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9356..9358,aa:His,seq:gtg)
     assembly_gap    9588..9687
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10212..10778
                     /locus_tag="PLW07_01275"
     CDS             10212..10778
                     /locus_tag="PLW07_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOL49187.1"
                     /translation="MKKNWIIAIIVIVLCLIFYQVLKFQYNRLIALDQKVKQSWAQVE
                     NVLQRRADLIPNLVATVKGYAKHEKELFIEVTNARSSLLAAKTVEEKSRASGDFEKAI
                     SKLLLVVENYPQLRASENFLRLQDELAGTENRIAVERKKYNDAVNDYNRIAKSFPVLL
                     FVHTFGFDTEKPYFQAESGSSQKPEVKF"
     gene            10778..10999
                     /locus_tag="PLW07_01280"
     CDS             10778..10999
                     /locus_tag="PLW07_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017295149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HOL49188.1"
                     /translation="MKISAKVKTLSREEKISQVEPGKFIVHVKTPPIEGKANEEVIKI
                     ISKFFKVPKSKVRIIAGHKSKNKIIEVLQ"
     gene            10996..>11334
                     /locus_tag="PLW07_01285"
     CDS             10996..>11334
                     /locus_tag="PLW07_01285"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOL49189.1"
                     /translation="MKRVYLYNHIKTGNKILISDEPAHYIKHVLRMNPGSTFCAFDGS
                     GNEYILKIKKISLNAIESEIVEEKYTEEKEPEISIELCLCLCKNKTFENILKKAAEIG
                     VSTIIPIKSAR"
BASE COUNT         3760 a         2147 c         2096 g         3231 t
ORIGIN      
        1 cccacctccg cggcagccat ctataaactc attttcctca gcatttccat caatagtaat
       61 gccctcaata accagattat tggcatagta tccgctgata acaggaaaga ctgttgaaac
      121 cattgctttt ctaattcttg cgtaatcatg attcaacatc cgattgattc caattctatc
      181 gccatctttc catgttacgg ttgccactgt accgtaaaaa ccacagccat ttacatccct
      241 gatgtataca cccattccgg gcttgaacaa gccgggcttt tcaacagata catcgtaatg
      301 gccatatccg agatcagctg aaagttttga tgattgagac ctgcactttt tcaaaatagt
      361 tttacctttc acaccaacaa catttactcc actgcgcaga tgaagagaat catacatatg
      421 gaatgtgcca gagcaaatcc tgacagtgcc accccctgat tgatacacat catcaatcgc
      481 cttttgcaat attttgttcg tccctgattt tgtttcaact tcaacaatct tcatcgcgtt
      541 ttgttctctt ttatttcatt atatttcatc ccatatttct tcaaggcatc taccagcggt
      601 ctggcactat ccggcatttc cacagtattg gtaaatagaa tatcctggac agtaatacct
      661 ttaataccat agaactgttc atttgcctta tcatcgttga ttatcaatcc tccttcaatc
      721 gtggctcctg cgtataatcc tctggcgcga gaataaacca gaaccgcggt tccaggtcca
      781 agcttcgctg acgcatccct gccaacagga cccgctgcaa cagaaccgtc aaccccgagt
      841 ttaaactttg ttttcatcaa catttccatc ccatctttat tcatgatgag cagcacagaa
      901 tcaaccgcct gaccacctat ctgaacacca taactgcctt ctccagattt tacaaacgcc
      961 ggtggactcc actcgcctgt tttttcatct tttgccatgg caataccata accaccctta
     1021 ataccaaaga taaaacctgc tttgtactgc ttcatgataa taattcccct gcacttttca
     1081 aataatgcag cagggatttc tgtgtcaggt gcttccatta tgtcgtcaag aaattctttc
     1141 gcctctgcaa ttcttttaac gagcgatttg ggcgcggcat aaacacatcc agaccaaaaa
     1201 agaaaaacca ttaagcacag tcctgcaacc tttgttttca taaaaccctc ctttttatct
     1261 gtattttctg atctcttcaa gagattttct tttcttttct ctgacttcac tatcagcagg
     1321 atacccgact gtaacaagga cgtcaatttt tcgtcttaaa ggaatgttca ggattttgtg
     1381 aatctccctt tcgttaaacc agccaatcca gcaggtgcca agaccttctt cggttgcctg
     1441 aagaataaag tgttcaacag caattcctat atcaatcaga ttgtattgag tcccgcgaaa
     1501 atatccaccg aggcatgcgg aatatcgcga cctctctgtg atcacaacaa taattaccgg
     1561 agcatttttc gcaaaactgt tgagcccata aatgccagaa aacgcccttt ctgccatttg
     1621 atttatcaat tctttatcag taatagcaat gaagctccac ggttgagaat tgcacgcaga
     1681 aggagcaatt ctcgctgcct caaaacaacg gtcaagaact tcttgaggta ctgaatcagt
     1741 cctgtattcc cttacacttt ggcgtatttg caccagttta agaaaattca tattctaatt
     1801 atagcacatt tttacaccat atttatctgg cgtgttgttg aacgaatatt gacgcaggtg
     1861 gaatttttgt taaaatactg gataaaaatt aaaaaccatg acaaattatc tatttgataa
     1921 aagttatgac attgtggtcg tgggcggtgg tctttctgga attggctatg ctatcgctgc
     1981 ggcaagtgga aaaaaagtac tggtcattga aagaagatct tcactcggat gggaggtctc
     2041 aaccgcattt tccagtgaat tcaatgggaa attatcaaaa actgggaaaa tcctgaaaaa
     2101 agaattggaa tccagacacg gaataaaaaa agataaagtt tcacctgcgc tttttgagat
     2161 ttcgcttgag tcgattctta aaaaaatgaa tattgacata atgttttata catatcccct
     2221 tgatatcata aaaagagatg atctgattgc gggattaatt gcgggtaata agaatggagc
     2281 cggcattata agtagcagga ttttcataga tgccactgaa aatttttccc ttttaaaaaa
     2341 atgctctgat agatttatgg cagacaagaa ttattctcat atttactata tttttttcaa
     2401 tggagtcaaa aaagaaatca aggatcagga aatcgtcaat agaaaaatca atgatattga
     2461 aattttatct ataaaaaaag gatttagaaa gggcgaggta tttgccgaaa ttgaaacaag
     2521 aaataaaaat atcacagaaa cgagaataca aattccaaca ttgatttctt ttctcaagga
     2581 aaacattgat tttttgaaag aatcccttgt ctcccacata agcaacgaac ctgtatgtag
     2641 caagccacaa atatcgtgta aatttagttc tttaatgagg aatctttacc cggtcattta
     2701 ttcggatgac gacaaacaaa actcaagttt ggtagtgcaa aaatttgaag caggtacaaa
     2761 aacagcagat tcactaaaaa acattgatga aatttttcct gatgatatat ctgtttttga
     2821 atgtgaaaga tatacaaact gtgatgaaaa atttgaattc gatgtcgtcg ttgctggagg
     2881 aggtactgca ggcgcagttg cagccatatc gagcgccaga gagggaataa agaccgctgt
     2941 tgttgaacat tcaaattgtc ttggtggaat tggaacaggc ggtggaattc accactacta
     3001 ctttggtatg ggtggagggc ttcaaaaaga aattgaagaa aaatccagtg aattgactaa
     3061 actgttcaca ggcaaattca aaattcacgg ttggcaccca gaggcaaaaa aaatcgtact
     3121 tgaagaaatg tgcaaaaatg caggtgtcaa agtattctat ggaaactgtt gtattgatgt
     3181 tgaaatgaaa gttaacgcaa tttctggcat ttattgtgct acagcagatg gaatgaaaaa
     3241 atataaagcg aatgttgtta ttgatgctac tggcgatggt gatatcgctg caaaggccgg
     3301 ggctcccttt accctgggaa gagttgctga caaattgcca caaccgttta ccctggttcc
     3361 agtagtactc acaccagacg gaacttttgc ctatatcaac tttgattccg gttttgttga
     3421 cccaacagat acattggatt taacaagagc aagaagacag tcattaatgt tgttcgaaaa
     3481 agaaattaca aatgaattag atatccccct ttacatctgt ccgatcattg gcataagaca
     3541 gggtagacaa attctcggga agtacgttct gactttaacg gatcaaatct ttggtgcaag
     3601 attttcagac gcagtgtgca gtatggactc acattatgat aaccattcaa aagatatgga
     3661 aaaccagagt tttcaatcat tgttatggag atggcttaca gataatttta tgacaagaat
     3721 agaatgtgaa ataccgtaca gatgtctctt acccttaaat gtgaaagggc tgattgttac
     3781 tggaagagcc atatcaatgg accctgaagc ccactatgca ttacgaatgg aaaaagatat
     3841 ggaacgactt ggagaggtcg ctggtatcgc tgcaagcatt tcagttaaaa atggagtatt
     3901 tcctgaagat attgacataa aaaaacttca ggaaaagcta aaagcaagtg gctgttggaa
     3961 tccagattat atgataaaat cagcgattaa catcaacagt tcgtttgaag atttgaaaca
     4021 aaagttcctg tcaaaagatt tcaaagaacc ggtgagcata tggactttta atttttctga
     4081 gaaggtcgaa aaattcttga atgcaattct aaaaaaacct gacgatacac aactacaatt
     4141 ctggagcacc acaatccttt caatccataa gaaaaaatca ggggttaaat ttcttattga
     4201 atggctgaag gaaaacaagg atatcaattc tcaattgtgt atttctatta ttacgattct
     4261 gggaagaacg aaagatagca tggcgcttga tcttttgcac ggtatactat ttgacaaaaa
     4321 tgcaaatgca gatacattaa tcgcaacaat acgggctatt ggtgaaatga ggaatgaaaa
     4381 atcaattgca gcgatagaca aatttctaaa aagaaaggat attcccaacg aaaggattct
     4441 gtcacaggga gtaccaggac tcataaaaaa ggtaaaagaa aatgcgttgt ggcagataga
     4501 acttgcctgt gctgaaacct taaaaaaact tggaaatttt agaaatgaaa ttgtggataa
     4561 gtatttaagt gacactcggg cttatgtaag aaactatgca aaaatgatca tggataaaca
     4621 aggagtagcg tatggttaaa aatcatgatg ggtgttatac agttgctgga tatcttgcag
     4681 aattatttga tagtgaaaag agaatacttg aactcaaagc gaacaataga gttgaatttt
     4741 tgcaatggaa aagcaaagcg aaaaaaaagt taaaggaatt gattggttac tactgcttca
     4801 aaagatgcaa tcccagtcca aaaattaatg aaaccgttga atgtgatggt tttactcgaa
     4861 tacatatgga aatttataca gaaccgtcca taagaatgcc gttctatgta ttgaaaccaa
     4921 aagacgaaag acagcgttat cctgttgtaa tggcattgca cggtcatgta agcggcggca
     4981 aatacgcagt agtaggcaga tatgatattc ctgaacttgt tgaatggatt aaactttgta
     5041 attatgatta tggtgtgcaa tttgtcaagc agggattttt ggtattttgt cctgatgcca
     5101 ggggatttgg agaaagacag gaacagtggg caaaaggaaa tattacttct ccttcatgtt
     5161 tctggctaaa taacatggca attccgcttg gaatgactgt aactggaatg tggacatggg
     5221 aggtacacag gttaattgat tacatcaaaa caagaaatga cgttgaaaaa gataccatcg
     5281 cgtgcgtggg actttcagga ggtgggttac aaacattgtg ggcaagcgca cttgatgaga
     5341 gaataaaatg ctgtgtaatc agtggctata tgtatgggta taaagaatcc ctgcttgaaa
     5401 tgtgcacaaa ctgttcctgc aattatgttc caaatcttta tcgttatttt gacatgggcg
     5461 acattgctgc attgattgct ccacgggcaa tgcttgttga aactggaaca aaagacgctc
     5521 tcaacggaaa aagtggtttg aaaaatgtgt tttcccaaac tgcaaagata aaaaaggcct
     5581 ataaaatttt taataaagaa gaatttttta aacatcatgt atttgatggt catcatcgtt
     5641 ggaacggcga agaagccata ccatgggtga aaaaacatct actgggacaa tgaattcaga
     5701 gaaaaaaatc ttaagtacag gcattatagg aatgggtgga tttgccgcag cacatcaccg
     5761 tgcaatcaat gaacttgaaa aaaagggcat ggtaaaacta tgctgcacct gtgatatagc
     5821 acctgaaaat ttcaagcaaa tgattgaaaa tctgtgtttt gaaaaaaggg gcgtggaaat
     5881 attcgataat tatctgaaaa tgcttgacgg ctgtgaagaa aaacttgatt ttgttaccat
     5941 tcctacgcca attccacttc atgccgaaat gcaccagcaa tgtgttaaga aacaaatccc
     6001 ggtttatctt gaaaaaccac ctacccttga ctaccagcaa tttatttcga tgatggaagt
     6061 agagcagtac gcaacgaaac aaaccaatgt tggtttcaat tttatcattc aaaaattgcg
     6121 acatagaata aaacaaagaa ttctttcaaa tgaatttgga aacataaaaa aaatcacctt
     6181 catggggcag tggccaaggc cttcatcata ctttagacga tcatcctggg ctggaaaact
     6241 taaactcaac aatgatttcg tccttgattc ctgttttggg aatgccatca gccattatgt
     6301 tcataacata ctcttctggt gcggcaaagg ggatattttt tcctgggacc ctgtggaaga
     6361 agttcacgct gaattgtacc gtacacatga cattcaggga acagatactg tatttgccat
     6421 cgcttcaacc agacatgtca acaatatcag aatcgcctta acgcatgcgt gtgcagaaaa
     6481 aacaagggat attgaaaaaa tatactgtga aaatgcagaa atccattttg aaggaaacgc
     6541 aaccggtaat aacggatatt gtgcgcaatg cacaatctat tggaataata gacagaagga
     6601 aatgattgaa gagccatacg agaatctttt aagagataat attgagtatt atgccaggta
     6661 cctgctgggt gaacatcaaa gaccgcttat acttctatca gatacagaac ccttcgtatt
     6721 attcaatggc ctcatttatg ttgcagcaaa aaaaattact cagataccag agcaatcaat
     6781 cgaacgaata aatctggata atggcgatta ttttttgtca ataaaaaaca tcaaggaaat
     6841 cgcacagaaa tttattgaaa caggaaaatt tccctcagaa caaaacatcc agtgggcgcg
     6901 atctggcggt cacgcaaaat gcgaccaaat caaggatttg catagaataa tatggaacat
     6961 aatttcgtaa atcatccaat caaaattgcc attccaataa atccaaacgt atatccccaa
     7021 ccaaaagttg aaaattgagg attatagtgc gcaagaacga atccgacgat tgtgactata
     7081 agaaaaacaa caagacgaat aaatttccat accgtcatct caatttttcc gaatttcttt
     7141 gtcataatcc cccctttttt attaatgata acctgacatg gtaattatga caaacaaagc
     7201 attgcaaaat ttttcggact gtataaaata agaaacatga accctggaat ttttcactat
     7261 tcgaaatcca ataatttcaa ggtcaggcag attaataaag caaaacagta tgatgtttta
     7321 gaggttacat ttcaatccag ccaaccaacc ggcatagacg tatgcgataa agtatctggc
     7381 ttctattaca gagtacctga tgccagaagg gctgttataa tcctgcacgg tctttccatg
     7441 atttctgtga caaaatttct atgtttgcat ctcgccagaa acggtttttc atcttttctt
     7501 atcataatgc cctatgctcc taaaagaatt cccagaaaac ggcccattag acacttaccg
     7561 caaaatcagg attgggttga aattttcagg cgcggcttaa ttcaggcagt tattgatgta
     7621 agaaaaacca ttgatttttt agaaaaagaa aatgaaaaaa taggaattct tggcataagt
     7681 cttggagcat ccatagcaaa tcttgtacat tcaattgatt caagaatctc ttcagggatt
     7741 tatgtagtag gaggcggaga cctggcaggg atgttatggg attcaaggga ctttatggca
     7801 aggatttata aaagaaaatt actgggttcc atcgataaac taacccttat gacacgatgg
     7861 aaagatatag acccgctaac ctatgccaga aaattagaaa atgtcttgat gattaacgcc
     7921 agatatgaca cgtctgtcaa acctgtatat accctgaaat tatggaaatc tgttggtaaa
     7981 cccgaaatat actggttgag atgtgcacac ttttttctct ctcacatctt ttttgtaaaa
     8041 cggcttattc tcaaacattt tagaaaaaca ctttcataag gaagcaagaa aaccaccatc
     8101 aatgataatg gtttgtccgg taataaacga tgcttcatca gaacaaagaa aaatcacagc
     8161 aggaccaata tcatcaggaa taccccatct tcccagaggt gttcttttta ccacccaatc
     8221 tgaaaatttt gaatcattat aaagcggctc agttaattca gtttttatgt atccgggtga
     8281 aattccattg acacgaattc catactttgc ccagtcaacc gcaaatgatt tcacaagttg
     8341 ttttaatgct gcctttgaag caccatacgc acaggtagta ggtcttgcta cttcagacat
     8401 taatgacaaa accataacac aacaaccccc ggaattttta ttgattaaag atttcgccca
     8461 ttgtgctgta agtacaaagg cacttgtaag atttacagca ataacctttt cccattcttt
     8521 gatggataat aaatccgctc ttttcctgac agtaattccg gcgcagttta caaggatatc
     8581 aaagacgtcg tatttacgtt gaagattttt ataaaaaaca ggaatttttt tataatttac
     8641 aaggtcaaat cgcaccgaat cacacctatc acaaattttt ttcaacttac aaagtgcgat
     8701 gttatttttt ttatcgttct tcccaacaat tacaactgtt gctcctgctt tcacaagtgc
     8761 actcgcaatt ccaaacccta taccagaatt accacctgtc acaagcgctt ttttcccctg
     8821 aagattaaat gccattgttt gtttcatttc gacatcacct tacatttcat aattttcact
     8881 ggaggcttat ctttttccca gcttctttga taagtaaatt caacaattgt ttcacctttt
     8941 tttaacgcct gaaaaataaa aacctgtttt ccaggacttc caaccatctt ctgtgattgt
     9001 ggtgaaatat attcacttcg ttttagattc aacatcattt cgtcatactt cgcgaaccat
     9061 tcataaccag ttgtgggatt ggacttaagt tcaattctaa atgtatcacc tgattttact
     9121 accaattcag tctccatttg attttcctct aataatgaaa ccgaaaaagc aatctccttt
     9181 tgaatacacc ctgcgacaaa agacatgatt aaaatgcctg caatgcacaa tgcactaaat
     9241 gttttcatga ttcctccagg ttaaaaaacc cattgtttat tttactcaat taaaattttg
     9301 tgtaaaataa tatacctgca tggtgggtgt agctcagcgg atagagcgcc agactgtggc
     9361 tctggatgcc gggggttcaa atcccctcac ccaccctttt tcatttttga caggggattt
     9421 gaatagagcg aagtcaggaa ttgcgagcga gcgcagcaat tccagcgaat ggccgacccg
     9481 agtcgtagtg ggagacgagg gcgccaaatc ccctcaccca ccctttttca tctttgacag
     9541 gggatttgaa tagagcgaag tcaggaattg cgagcgagcg cagcaatnnn nnnnnnnnnn
     9601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9661 nnnnnnnnnn nnnnnnnnnn nnnnnnncct ttttcatctt tgacagggga tttgaataga
     9721 gcgaagtcag gaattgcgag cgcatattct gaaacacatc ggttacacag tatggtaaat
     9781 taacctcagg caatcagggt taatttgaat agaaacattg atagccagtg tatcgatatt
     9841 cgcattttct cctctcgtaa aattacctcc tttttttccc cctttacaaa accacctggc
     9901 aattttcccc ttttgtaaaa ttcctctctc catttccatt ttttcaaaat ttctccactc
     9961 gttttccccc tttatgaaat actttctttt actctccccc ttttgaaaag ggggacaaag
    10021 ggagattaga aatcttcccc aacccctctt ttacaaagat gggggtagaa tggagattga
    10081 caaagaaagg agcctcatta ataagacaaa catatctatg tttttcatat aagcagacaa
    10141 accattttat ttttttcttt cattgatgta taatttgaaa gaaaaaccca aagaaaacac
    10201 gaggaggaaa tatgaaaaaa aactggatta ttgcaataat agtgattgtc ttgtgtttga
    10261 ttttttatca ggtgttgaaa tttcagtaca atagactcat tgcccttgat cagaaagtca
    10321 aacaatcctg ggctcaggtt gaaaatgttc ttcaaaggcg ggctgatttg attccaaatc
    10381 tggtcgcaac agtcaagggc tacgccaaac atgaaaaaga acttttcatt gaagtaacta
    10441 atgcaaggtc ctctttactt gcagcaaaaa cagtagaaga aaaaagccgc gcctcaggag
    10501 attttgaaaa agccatcagt aaacttcttc tggtggttga aaattatccc caattaaggg
    10561 caagtgaaaa ttttttacgc cttcaggacg aacttgcagg aacagaaaac agaattgcag
    10621 ttgaaagaaa aaaatacaat gatgctgtga acgattacaa tagaatagca aagagttttc
    10681 cggtgttatt gtttgttcat acgtttgggt ttgacactga aaaaccatat ttccaggcag
    10741 aaagtggatc ctcgcaaaag cccgaggtta agttttgatg aagataagcg caaaagtgaa
    10801 aacattatcc agagaggaaa aaatctcgca agtagaaccc ggtaaattca ttgttcatgt
    10861 caaaacgccc cccattgaag gtaaagcaaa tgaagaagta ataaaaatca tttcaaaatt
    10921 tttcaaagtc ccgaaatcaa aggtaaggat tattgcgggg cacaaaagta aaaataaaat
    10981 tattgaagtt ttgcaatgaa acgagtatat ctttataatc atataaaaac tgggaacaaa
    11041 atccttattt cagatgaacc agcacattat atcaaacatg tactgaggat gaaccctggt
    11101 tcaacattct gcgcatttga tggaagcggg aatgaataca ttcttaaaat caaaaaaata
    11161 tctttgaatg ccattgagtc agaaatcgtt gaagaaaaat acacagagga aaaagaacca
    11221 gaaatttcaa ttgaactctg tctttgcctg tgcaaaaata aaacatttga aaatatcctg
    11281 aaaaaagcag cagaaatcgg agtttcaaca atcatcccga ttaaaagcgc gagg
//
LOCUS       DAORYU010000020        11032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_4519_length_11032_cov_4.480004, whole genome shotgun sequence.
ACCESSION   DAORYU010000020 DAORYU010000000
VERSION     DAORYU010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11032
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4519_length_11032_cov_4.480004"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="PLW07_01290"
     CDS             <1..419
                     /locus_tag="PLW07_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49190.1"
                     /translation="AFLPGLSYFKPAIPLKPVDRGSTDDAMAHFIPVDFEKNTGNLIK
                     SACSGNMPVFVKDTKGNPLGCIETTIIESKNGMVIPVINWSTNNVSDAIFEITFNLSG
                     KKISLASGKPIKAVRITKEKAEIVFDVELADAIIIR"
     gene            429..1622
                     /locus_tag="PLW07_01295"
     CDS             429..1622
                     /locus_tag="PLW07_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49191.1"
                     /translation="MWYNRDEFKTFAMPDPVKDAMEEAQRINANRYYYLESGIERIIA
                     DAPHIKKQELVQVVENYYKEAMASIPSFVKFPEAKTWADYVITRDKKLVEFANLTGQE
                     IAIFRSTNDYLTFRGYKEFGLKMICCDEKCRVVFIPETDQGPMHIKNVDDPITWWSPS
                     RRLDERAPISQAFWYHRNVVIDGVGSGLHIDDEPDEIFPLPVMKMVDMYAYDTDSAVD
                     FLRKYSVFWGGCNVIIYDKKYRSVAIEKCSRNFFEPFQPEEPSHFTHISGMVCRDKGS
                     PQGQYQTEKRHQYRKLFGLADDGPDALFWKACDNLEQILSKGIENLGKHPVSEDVVKL
                     FITPYPSGLRKDGLKLHPQQGLVDYTLVTNCFFPEKKLYYRWQRTSEKDRGNWPGQPE
                     ICQYE"
     gene            1636..2568
                     /locus_tag="PLW07_01300"
     CDS             1636..2568
                     /locus_tag="PLW07_01300"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOL49192.1"
                     /translation="MFSNEFKKRMESFSSKDEIVAFNLAMVSEGLEIVKNLSDKNIGW
                     EEVEKRLGEYKNWDPDILRVDKDTENTYRKRLEEWGKPIVLLSEEAGRVEINTDKSGS
                     PLYVVCDPFDGSYLFKHGIKDFWYSSLAFYDSNFNPVCCCVGDCVSGKIAYAWKNGAY
                     LADIEHGKIVTEVKLDKKYRQSMGRPDVTEMDGASIESYAMKPKKFLIPLVDKYRELL
                     LPFKFFLPNGGPYGFVDVAEGKIDVYFAMKQPFVDVFSGIFIAQQSEIMVTDFNGNPV
                     RCSDNVKTVLDVVVSTNASLHEKILKKIAGCENR"
     gene            complement(2572..3540)
                     /locus_tag="PLW07_01305"
     CDS             complement(2572..3540)
                     /locus_tag="PLW07_01305"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HOL49193.1"
                     /translation="MIQKDITTALLTLHRRVAKDLKKEHVFNKVAETVRSLIRSDGCA
                     ILLMDNNTISVVASIGFSKKTPEFSASMKPIRDIIRTGKGFINNDVEKSKLKSCLPAG
                     CHMKSILCFPIKQNGQVAGIIHLDSKKKNAFTEDDLNFVKIVSSELTSIMERSFLYSK
                     LEEMSIKDPLTGCFNRKNISLDIKKEIERCKRYKKTFSLLVIDLDNFKKYNDTFGHHR
                     GDNLLKTIAKKIRRNLRKADVVYRYGGDEFVVLLPETGKTGAMVCAKRLEDMIEKLNM
                     KVDKHTRITLSTGMAGYPENGDTPTKIIKFADRQMYRNKFLKKAGR"
     gene            complement(3544..4935)
                     /locus_tag="PLW07_01310"
     CDS             complement(3544..4935)
                     /locus_tag="PLW07_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HOL49194.1"
                     /translation="MKKSIFIVAILTISVSAQVMEKKQISLTVYNQNFALVRDVRTVQ
                     LKEGLNTVRCSDIAAQIEPASVSFKSLTAPDRCLILEQNFEYDLMNADKLLKKYIDKK
                     IKVMTKDNNTYSGLLSSYDEKYICVVQQENGPVYMLTRENIRDIEFPLLPEGLITKPT
                     LVWNLSSAKTTQDTVELGYITGGLNWQADYVAIVSKEDTYISLNGWVTIDNRSGTDYE
                     NAELKLMAGDVRKISEQPATRTLMMEAKASGAAQFEQKEFFEYHLYTLGRKTTLKNNQ
                     TKQISLFSAPKIGIDKTYTYEGALYRWYYYDNWQTQQSNKKVAVKIELKNTEKNGLGI
                     PLPRGKVRVYKTDTDGSMQFIGEDIIDHTPKDEKVILSLGNAFDIAGERKITDHKKIA
                     ANIFRDTYEIRLRNHKKESVNVKIVERQFGEWSIIQTSHPYEKRDSETVEFNVMIPVD
                     GEVIVTYTSEYKF"
     gene            5021..5956
                     /locus_tag="PLW07_01315"
     CDS             5021..5956
                     /locus_tag="PLW07_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49195.1"
                     /translation="MADIIKLNEVSRGDLPERLRVEFYFDFKKYPFRHQDLFTRPDVI
                     SVISALDAIHPYACEWIKKAIDEKKKTSALKEMGPEHFKGRCIGEFIIYVEDGAVFEP
                     GFIRGSLKNNKHTLFVGKQTVLAGTNIFLDEGDIYIGEQNSVEPGAGIKGPTIIGNKN
                     DIRQGAYFRGDVIIGDGGTFRGEIKNGVMMDKANFPHPSYVGDSICGYATHFGNQGTT
                     ANVGIYAGVFGKKNVVIELCDMKYDIGRPKIGIILGDWSQLGCNSVSDPGVFVGPNTI
                     FYSLCRISKGFYGPNEVLKNKPLEHGVIERSSLRT"
     gene            complement(5953..6816)
                     /locus_tag="PLW07_01320"
     CDS             complement(5953..6816)
                     /locus_tag="PLW07_01320"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOL49196.1"
                     /translation="MKKRAIVFDFDGPLVGSGQDKAIHILFSAFVACWDTDFRKFLHP
                     GHLHLDISRMLKALVNYPGAPRFQQLSAIVSAIVRDIPEAFADVRELGIDETLVNEYQ
                     KLQQRYNEFYSGLNEAAAKMFWKPLKGVKTLIKKLSKDYDLYVASGILQDILEKDFEH
                     HGFDRKLFCGIMGSNPEGTVDKSVILKQIKSRPYQDILFVGDSKKDFEYASIAGVKFF
                     RVKSAKDYNRFVEKLKQCLPDEQEMCEFTDQELQRIRLKTLKLMNLYASGKILSYSEI
                     TSFINSKNRKS"
     gene            7465..9432
                     /locus_tag="PLW07_01325"
     CDS             7465..9432
                     /locus_tag="PLW07_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49197.1"
                     /translation="MLKLTKKEKKITVETGSGKLTWDANKGGEIVEFSCKNEKESHKL
                     LSFPHSVCGLKFIIDGSPYLLSNTVSQLTVESVDNDRIVIKNEASIAKGLIKITQTYQ
                     VFEEGAVFCEQVFDVEGNTGFTLNEISMNTSIDVATAKSARWGYFTRQPWYKRDYSTT
                     HVVYGLNLYKQLDEISCHKELWPLVSIDLGWENTKFFSNKIEFVLDEWNSFNDADTKY
                     TMSYGGKQGEQWACQWYIFHGTQMRVSEPVRYRNKWGMLFLTSRTRSGTNVDPAYRNN
                     VLGCRVAHCMYPYAQIGKDWPWALMPIKQVACQPPQLFNGNPDIKRVDEVADTGANVM
                     IIHQFWMRNPGTNCEPPADYIVKDPDWFRAFIDRCHQRGMRVLTYIRGCEQYQMYQTW
                     FEDFMKRDWDGFYPDWNSPHAMGFTKTSLLHWSAYGYFMYLKAMRKRVGNYGAIIGHT
                     GFPFALSQSCIDVALCGELSIRHDELLELPESTAYYSCLDCSGAHLIAGNLKDRAMFS
                     GKKAAAICAAFGMTGHPVLEPGVPFEKAVAYIKPLWDAMTSINGNYVKLHNPAYCPTH
                     AVHCDQYWLFPSLWQSDQGKCLLLVTNLADNEKASGKIIVETEELDVSRKPKMRVLNI
                     AGTVPQSIEIKGKMLKINNLPPLSFCAVIIE"
     gene            9544..>11032
                     /gene="serA"
                     /locus_tag="PLW07_01330"
     CDS             9544..>11032
                     /gene="serA"
                     /locus_tag="PLW07_01330"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOL49198.1"
                     /translation="MKIKVLATDNIEKEGIEILVKEGFIVDEKGKMSEEELLAVIEDY
                     DVVLVRSTTKITSKVIQKGKKLKIIGRAGVGLDNVDVESATLNGIIVMNAPEGNTLSA
                     AEHTFGLILAMARKIPAACSCLKTGKWDRKKFMGTELFGKTLGVIGLGRIGRRVAHYA
                     KAFGMNVLGYDPYVDQDEMQQVGISVVTLEGICKQADIITLHLPLTKETENILSEQCF
                     SMMKDGVMIVNVARGGLFDEAILEKYIKNGKVSAAALDVFATEPPDCTGLLGLENVIA
                     TPHLGASTKEAQINVAKDICRQVVDALVHKIIRNAVNVPSIDKEVISRFGGYLTLAEK
                     MGLFLGQMADKMPDGLTIEYAGEMIDYDTGPLRAYFLKGFASHFQQATYVNAPLILKE
                     KGVGLVEKKTMSAQQYANLITTEAAIGGKTVSLSGTIVASEPKIVSIDGYPVEATPEG
                     TLIVCRNDDVPGIVGHVGTILGKKGVNIASMTMGRRVKGGPAITVL"
BASE COUNT         3300 a         2024 c         2382 g         3326 t
ORIGIN      
        1 gcgcatttct tcctggattg agttatttca agccagcaat acctttgaaa cctgtagaca
       61 ggggttcaac agacgatgca atggctcatt ttataccggt tgattttgaa aaaaatactg
      121 gcaatctgat aaaatccgcc tgttctggaa atatgcctgt ttttgttaaa gacactaagg
      181 gcaatcctct gggatgtatt gaaacaacaa tcattgagtc aaaaaatgga atggtgatac
      241 ctgtgatcaa ctggagtaca aacaatgttt ctgatgccat ttttgaaatc acattcaatc
      301 tgagtggaaa aaaaatatca cttgccagcg gaaagcccat taaagcagtc aggataacta
      361 aggaaaaggc agaaatagtg tttgatgttg aattagcaga tgcaataatc ataaggtaaa
      421 gggggtttat gtggtacaat cgcgatgaat ttaaaacatt tgcaatgcca gatcctgtca
      481 aagatgctat ggaagaggcg caaagaatca atgcaaacag gtactattat cttgaatcag
      541 gtattgaaag gattattgcg gatgccccgc atataaaaaa acaggaattg gtgcaggtag
      601 tggaaaatta ctataaagag gcaatggcaa gcattccctc ttttgtgaaa tttccagaag
      661 caaaaacatg ggcggattat gtgattacca gggataagaa acttgtggaa tttgcaaatc
      721 tcacagggca ggagatagcg atattcagaa gtaccaatga ttatctgact tttcgtggat
      781 acaaggaatt tggactgaaa atgatatgtt gtgatgaaaa atgcagggtt gtttttattc
      841 ctgagacaga ccagggaccc atgcatatta agaatgttga tgaccctatt acctggtgga
      901 gtccttcaag gcgtcttgat gaacgagcac cgatttcaca ggcattctgg tatcatagaa
      961 atgttgtgat cgatggcgtg ggctcaggac tgcacattga tgacgaacca gacgaaattt
     1021 ttccactgcc tgtaatgaaa atggtagata tgtatgcgta tgatacagat tctgcggttg
     1081 attttttaag aaaatattct gtattctggg gtggttgcaa tgttataatc tacgataaaa
     1141 aatacagaag tgttgccatt gaaaaatgca gcaggaattt ttttgaacct tttcaacctg
     1201 aagagccctc tcattttact cacatcagcg gaatggtctg cagggataaa ggctcaccac
     1261 aggggcaata tcaaactgaa aaaagacacc agtatagaaa actttttggt cttgcggatg
     1321 atggtcctga tgctttattc tggaaagcat gcgacaattt ggagcaaata ttaagcaagg
     1381 gtatagaaaa tttgggtaaa catccagtat ctgaagatgt ggttaaacta tttatcacac
     1441 cataccccag tggtctgaga aaagacggat taaaactgca tccacaacag ggcctcgttg
     1501 attataccct tgttaccaat tgtttttttc ctgaaaaaaa attatactat cgctggcaaa
     1561 gaacctcaga aaaagacaga ggaaactggc caggacaacc cgaaatctgt cagtacgaat
     1621 aaccgaggag gtgctatgtt cagtaatgaa tttaagaaaa ggatggaaag tttttcatca
     1681 aaggatgaaa tagtagcgtt caatcttgca atggtgtcag aaggactgga aatagtaaaa
     1741 aacctcagtg acaaaaatat tggctgggaa gaagtagaaa agaggttggg agaatacaag
     1801 aactgggatc ctgatattct cagggttgac aaggatacag aaaataccta tagaaaacgg
     1861 cttgaagaat ggggaaaacc aatagtactg ctttcagaag aagcaggaag ggttgaaatt
     1921 aacacagata aatctggtag tcccctgtat gtggtgtgcg acccttttga tggaagttat
     1981 ttattcaagc acggtatcaa ggacttctgg tatagttccc ttgcttttta tgacagcaat
     2041 tttaatcctg tctgctgctg tgttggagat tgtgtcagtg ggaaaattgc atatgcgtgg
     2101 aaaaatggag cgtatcttgc ggatattgaa catggaaaaa ttgttacaga agtgaaactg
     2161 gataaaaaat acaggcaatc aatgggaaga cccgatgtta ctgaaatgga tggagcaagc
     2221 attgagtcgt acgcaatgaa accaaaaaaa tttttaattc ctcttgttga taaatacaga
     2281 gaacttttgc ttccatttaa gtttttcctt cccaatggtg gcccgtacgg atttgttgac
     2341 gttgcagaag ggaaaataga tgtgtatttt gcaatgaagc aaccatttgt tgatgtgttc
     2401 tcagggattt ttatcgcaca acagtcagaa ataatggtaa cagatttcaa tggtaatcca
     2461 gtcagatgca gcgacaatgt gaaaactgtt cttgatgtgg ttgtctcaac caatgcttca
     2521 ttgcacgaaa aaatactgaa gaaaattgct gggtgtgaaa acagatagat tttaacgacc
     2581 ggcttttttt aaaaatttgt ttctgtacat ctgcctgtca gcaaacttga tgatttttgt
     2641 tggtgtatca ccattttcag gatatccagc cataccagtg ctgagggtaa ttcttgtatg
     2701 tttgtccact ttcatgttaa gtttttcaat catgtcttca agccgttttg cgcaaaccat
     2761 tgcacctgtt tttcctgttt ctggaagaag cacaacaaac tcatctccac catacctgta
     2821 tacaacatca gctttcctca ggtttcttct tatctttttg gcaatggtct tcagaagatt
     2881 atcacccctg tgatgaccaa aggtgtcatt gtattttttg aaattgtcca aatctattac
     2941 gaggagtgaa aaagtttttt tgtatctctt acagcgttct atttcttttt ttatgtcaag
     3001 ggagatgttt tttctgttga aacaaccggt caatgggtct ttgattgaca tttcttcaag
     3061 ttttgagtaa aggaaactcc tttccataat ggatgtgagt tctgatgaaa caatcttcac
     3121 aaaattcaaa tcatcttctg taaatgcgtt tttctttttt gagtcaaggt ggatgatgcc
     3181 tgcgacctgt ccgttttgtt tgatgggaaa acataaaatg gatttcatgt ggcaaccagc
     3241 aggcaagcaa cttttcaatt tggacttttc aacatcattg ttgataaatc ccttaccggt
     3301 tcttattata tctcggattg gtttcattga agcagaaaac tcaggcgttt tctttgaaaa
     3361 gccgatgctt gcaacaacag aaatggtatt gttgtccatc aatagtattg cgcatccatc
     3421 agacctgata agagacctta ctgtttccgc aaccttgtta aacacatgct cttttttcag
     3481 gtcttttgca acccttctat gaagcgttag aagcgcggta gtaatgtcct tctgtatcat
     3541 atcctaaaat ttatactctg acgtatatgt aacaataact tctccatcaa ccggtatcat
     3601 aacattaaat tcaactgttt cagaatctct tttttcataa ggatggcttg tctggatgat
     3661 tgaccattct ccgaactgtc gttcaacaat ttttacattc acggattctt tcttgtggtt
     3721 tctcaatcgt atttcataag tatccctgaa gatgtttgcc gcaatttttt tgtgatccgt
     3781 aatttttctt tcaccagcga tatcaaatgc attgccaagc gagagtatca ctttttcatc
     3841 cttcggggta tggtctatta tgtcctcacc gataaactgc atggagccgt ctgtatcagt
     3901 tttatacacc cttacttttc ctcttggtag aggtataccg agaccatttt tttctgtatt
     3961 tttcaactca atcttcactg ccaccttttt gttgctctgc tgtgtttgcc agttgtcata
     4021 atagtaccag cgataaagtg ctccttcgta tgtatacgtt ttatcaatgc cgattttcgg
     4081 cgccgaaaaa agactgattt gttttgtctg attgtttttc aatgttgttt tccttcccag
     4141 tgtgtagaga tggtattcaa aaaattcttt ttgttcaaac tgagcagcac cagatgcttt
     4201 tgcttccatc ataagagtcc ttgtcgctgg ttgttctgat atttttctga cgtccccggc
     4261 catcagtttc aattcagcgt tttcataatc tgtaccactt ctgttgtcta tggtgaccca
     4321 tccgttgagt gaaatgtatg tatcctcctt tgaaactatt gccacataat ctgcctgcca
     4381 gtttaaccca ccagtgatgt accccagttc aacggtgtcc tgggttgtct ttgcgcttga
     4441 taaattccag acaagggtcg gttttgtaat cagtccttct ggaagcaagg gaaattctat
     4501 gtctctgata ttttcccttg tcagcatata caccggtcca ttttcctgct gtacaacgca
     4561 gatatatttt tcatcataac ttgataataa tccagaataa gtattattat cctttgtcat
     4621 caccttaatt tttttatcaa tgtatttttt cagcagttta tccgcattca tcaggtcata
     4681 ctcaaaattt tgctcaagta ttaaacatct gtcaggtgca gtaagggatt taaaagatac
     4741 actggctggt tcaatctgtg ccgcaatgtc agagcacctt actgtgttga gtccttcttt
     4801 caactgaact gttctgacat cccggacaag ggcaaaattt tgattgtaca ctgtcaggga
     4861 tatctgtttt ttctccatca cctgcgctga aacagatatt gtgaggattg ctactataaa
     4921 aatggacttt ttcatctgcc ctcctggttc tattggtatg ataaatctat tgtcatatcc
     4981 tgtcaattga gatataatta ttccatcaca aggagacaaa atggcagata ttatcaaatt
     5041 aaatgaagta tcccgaggtg accttccaga aagattaagg gttgagttct attttgattt
     5101 caaaaaatat cccttcaggc atcaggacct ttttacacga cctgacgtaa tatctgtgat
     5161 ttcagcgctt gacgccatcc atccatatgc atgtgaatgg ataaaaaaag ccattgatga
     5221 aaagaaaaaa accagtgctt tgaaagaaat gggacccgaa catttcaaag gaagatgcat
     5281 cggagagttt attatttatg ttgaagatgg cgctgttttt gaacctggtt ttatcagagg
     5341 atcattgaaa aataataaac acacattatt tgtcgggaaa caaacagtgc ttgcaggaac
     5401 aaacatattc cttgacgaag gcgatattta tatcggagaa caaaattccg ttgaaccagg
     5461 tgctggtatt aaagggccga caatcattgg taataaaaat gacatcagac agggagcata
     5521 tttcagggga gatgtgatta ttggcgatgg aggaacattc cgcggcgaga taaaaaatgg
     5581 cgtgatgatg gacaaggcaa acttcccgca cccatcctat gtgggagata gtatctgcgg
     5641 ttatgccact cattttggaa atcagggcac cactgcaaat gttggaattt atgcaggagt
     5701 ttttggaaaa aagaatgtgg tgattgaact ctgtgacatg aagtatgata ttggaagacc
     5761 caaaataggt attattcttg gcgactggtc tcaactcggg tgcaattcag tttcagaccc
     5821 tggtgtgttt gtgggaccca atacgatttt ttattctcta tgcagaatca gcaagggttt
     5881 ttatgggccc aatgaagtgt tgaaaaacaa gccacttgaa cacggggtaa ttgaaaggtc
     5941 ttcattgagg acctaactct ttctgttttt tgagttgata aaactggtaa tttcactgta
     6001 agacaggatt tttcctgagg catatagatt catcagtttc agtgttttta acctgatgcg
     6061 ttgcaactcc tggtcagtaa attcacacat ttcctgttcg tctggcagac actgtttcaa
     6121 tttttcaaca aacctgttgt aatcctttgc acttttcacc ctgaagaatt ttactcctgc
     6181 aatacttgca tattcaaaat ccttctttga atcaccaaca aacaaaatat cctgatacgg
     6241 cctgcttttt atctgtttca gtatcacgga tttgtcaaca gttccttctg gattgctgcc
     6301 cataatcccg caaaaaagtt ttctatcaaa accgtggtgt tcaaagtcct tttcaagaat
     6361 gtcctgaaga atgccagaag ccacatacaa atcataatcc tttgaaagtt ttttgatgag
     6421 ggtttttacc cctttcagtg gcttccagaa cattttcgcc gcagcttcat ttagaccaga
     6481 ataaaattcg ttataccttt gctgcaattt ctgatactca ttcaccagtg tttcatcaat
     6541 accaagttcg cgaacatccg caaatgcctc tggtatatcc ctgacaattg cactgacgat
     6601 agccgacaat tgctggaaac ggggtgctcc aggataattg acgagcgcct tcaacattct
     6661 tgaaatatcc agatgaagat gccccggatg gagaaatttt cgaaaatcag tgtcccagca
     6721 tgcaacaaat gcggaaaaaa ggatatgaat ggctttatcc tgcccgcttc caaccagcgg
     6781 tccatcaaaa tcaaatacaa ttgccctttt tttcatgtca acaattatac tataaaaatc
     6841 tgcctttgta atatggaccg tatttctgta ctgcccccca tttatcaaga gcaaaataag
     6901 ggagaactta gggtcctggt taacggtgat tgttttatct ctttttgttc aaattccact
     6961 agtgtcatat atccaatgat gaatggcgat tgtcataact ggcaaatatc tgcttgattt
     7021 ttgatgtcgg ctgtgactca ttattatcac tgaccataga caaaattcat ctttactgaa
     7081 ggggaagcag tataattttc tctcttttca aaatcctcct ttaattctcc ctttataaaa
     7141 gggagatgta agagggattt cataaatctt ccccagcccc tcttttataa agaggggagt
     7201 aattttataa aggtgggaaa tagtatgagt tgattttata aaaaggatgt gatgttatcc
     7261 tgcttacctt ttcggaccac tctattctac cttattttcc acttcccctt aatgggcagc
     7321 agcataggta caggagggta cttatttttg aggaaacaac acactttcat agagattgtt
     7381 tttgaggaaa ggcgtggact ggacatttga aaaattctgt tgtattatca agttttgtta
     7441 agaaacaaaa agggggctac ggatatgttg aaactgacaa agaaagagaa aaaaattact
     7501 gttgaaacag gttctggaaa actgacgtgg gatgcaaaca aaggcggtga aatagtggaa
     7561 ttttcatgta agaatgaaaa agagtcccat aaattacttt cttttcctca ctccgtttgc
     7621 gggctgaagt tcatcataga tggcagccct tatctattgt caaacacagt gtcacagttg
     7681 actgtggaat cagtggacaa tgacagaatt gtgataaaga acgaagcatc tattgcaaaa
     7741 ggactgataa aaataacgca gacataccag gtatttgaag aaggtgctgt tttctgcgaa
     7801 caggtttttg atgtggaagg caatactggt tttaccctga atgaaatttc tatgaacaca
     7861 tccattgatg tcgcaaccgc aaaatctgca cgatggggat attttacaag acaaccgtgg
     7921 tataaaaggg actattccac cactcatgtt gtatatggcc ttaaccttta taaacaactt
     7981 gacgaaatat catgtcataa agaactatgg ccactcgttt ccatagacct tggctgggaa
     8041 aacacaaagt ttttcagcaa taaaattgag tttgttcttg atgaatggaa cagttttaat
     8101 gatgcagaca ctaaatatac aatgagttat ggaggaaaac agggcgaaca atgggcatgt
     8161 cagtggtata tctttcatgg cactcaaatg agggtttcag aacctgttcg ttacaggaat
     8221 aaatggggaa tgttattttt aacatcccgt acacggagtg gtacaaatgt ggaccctgcc
     8281 tatagaaaca atgtccttgg ttgcagggtt gctcattgca tgtatccata cgcccagatt
     8341 ggaaaggact ggccatgggc attgatgccc attaaacagg ttgcctgcca gccgccacag
     8401 ttgttcaatg gaaacccaga tattaaaagg gttgatgagg tagcagatac tggtgctaat
     8461 gtgatgatta ttcatcaatt ctggatgagg aatccaggta caaactgtga gccgcctgcc
     8521 gattatatcg tgaaagatcc tgattggttc agggctttta tagatagatg ccaccagcgt
     8581 ggcatgcgtg ttcttactta catcaggggt tgtgaacagt accagatgta tcagacctgg
     8641 tttgaggatt tcatgaaaag ggactgggat ggtttttatc ctgactggaa ttctccgcat
     8701 gcgatgggtt tcacaaaaac aagtttactt cactggtctg cttatggtta cttcatgtat
     8761 ctgaaagcta tgagaaaaag ggttgggaat tatggcgcga ttatcggcca tacgggtttt
     8821 ccgtttgccc tgagtcagag ctgtattgat gttgcgcttt gtggagaact gtccataagg
     8881 cacgatgaac tgctggaatt acctgaaagt acagcgtatt attcctgcct tgactgttca
     8941 ggagcacacc tgattgccgg taatcttaaa gacagggcca tgttttcagg aaaaaaagct
     9001 gcagcgatat gcgctgcctt tggtatgacc ggacatcctg ttttagaacc aggtgttcct
     9061 tttgaaaagg cagttgcata tatcaaaccg ctctgggatg ctatgaccag tattaatgga
     9121 aattatgtga aactccataa tcccgcttac tgcccgactc atgcggtgca ttgcgaccag
     9181 tattggttgt ttccaagttt atggcagtca gaccagggaa agtgtctact gctggtgacc
     9241 aatcttgctg ataacgaaaa agcaagcggg aaaattattg tggaaacaga agaacttgat
     9301 gtttccagaa aaccaaaaat gcgtgtattg aacatagcag gcacagttcc acaatcaatt
     9361 gaaataaaag gaaagatgct caaaattaat aacttaccac cgttgagttt ctgtgcggtg
     9421 attatagaat aatactgcat ttttgttgag ggtcaggtat ttatctgggt gtttccatcc
     9481 attcatgcat gcattttttg acacatagcc ctgtcgtggt aaaataaaaa tacaggagat
     9541 tttatgaaaa ttaaggttct tgcgactgat aacattgaga aagaaggcat tgaaatcctt
     9601 gtcaaagagg gtttcattgt tgatgaaaag ggaaagatgt ctgaggaaga gctcctcgct
     9661 gtgattgagg actatgatgt tgttcttgtg agaagcacca caaaaatcac atcgaaggtg
     9721 attcagaagg gcaaaaaact caaaattatc ggtcgggcag gtgtcggtct tgacaatgtt
     9781 gatgttgaaa gcgccaccct gaatggcatc attgtgatga acgctccaga gggaaatacc
     9841 ctttctgctg ctgaacatac atttggtttg atccttgcaa tggcacgaaa aattccagca
     9901 gcgtgcagtt gtttaaaaac tggcaaatgg gacagaaaaa agtttatggg aactgaactt
     9961 tttggaaaaa ctcttggtgt tatcgggctc ggaagaatcg gcaggcgggt ggctcattat
    10021 gcaaaggcat ttggaatgaa tgtgctgggg tatgaccctt atgttgatca ggatgaaatg
    10081 caacaggttg gtataagtgt tgtgaccctt gaaggcatat gcaaacaggc agatattatt
    10141 acactccatc tgccacttac caaagaaaca gaaaatattc tgagtgagca atgtttttca
    10201 atgatgaaag atggggtaat gattgtgaat gtggcaaggg gtggactttt cgacgaagcc
    10261 attcttgaaa aatatattaa aaacggaaag gtgtctgctg cggctcttga tgtgtttgct
    10321 actgaaccac ctgactgcac tgggctgctc ggtttggaaa acgtgatagc aacaccacat
    10381 cttggcgcat ccacaaagga agcacagatt aatgtggcaa aggacatatg caggcaggta
    10441 gttgatgccc ttgtccacaa aatcataaga aatgcggtaa atgttccgtc cattgacaag
    10501 gaggtaatat cacgatttgg aggttatctg acccttgctg aaaaaatggg gttatttctc
    10561 ggtcagatgg cagataaaat gcctgatggt ttaacaatag agtatgcagg agaaatgata
    10621 gattacgaca caggtccact gagagcatat tttctaaaag ggtttgccag ccacttccag
    10681 caagcaacct atgtgaatgc acccttgatt cttaaagaaa agggagtggg tcttgttgaa
    10741 aagaaaacaa tgtcagcgca gcagtatgca aatcttatta caaccgaagc agcaattgga
    10801 gggaaaacag tttctctgtc aggtacaatt gtcgcttcag aaccgaaaat tgtcagtata
    10861 gatggatacc cggttgaagc aacgcctgaa gggacactga ttgtatgcag aaatgatgat
    10921 gtgcctggaa ttgtgggaca tgttggcaca atcctgggca aaaaaggggt gaatatcgcg
    10981 tccatgacga tgggacgcag ggtaaaaggt ggcccagcga tcacggtttt ga
//
LOCUS       DAORYU010000021        10824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_4621_length_10824_cov_4.339957, whole genome shotgun sequence.
ACCESSION   DAORYU010000021 DAORYU010000000
VERSION     DAORYU010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10824
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4621_length_10824_cov_4.339957"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="PLW07_01335"
     CDS             <1..258
                     /locus_tag="PLW07_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49199.1"
                     /translation="IVNPIPNGTTYILNSASGKDTEISFSIDGGKYFQKPPVKYLVKK
                     PDGSTEEKIATPEMYTHIMWTIKKPIPAGASGEVSFSVKVK"
     gene            365..2122
                     /locus_tag="PLW07_01340"
     CDS             365..2122
                     /locus_tag="PLW07_01340"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49200.1"
                     /translation="MKKLLLLAVGLLAAGMFIAPSAMALGTPAGTVISNQAYVDYKDA
                     NGNPKTRVYSNTVTTVVSQVAGVSIEPATAAKSGLAGGTVYYGVTVCNTGNGPDTINL
                     ALSGNTWAAVIYFDTNGDGIWDPVNETTTVTNTGVLAADACYKVLVVVTIPSGVANGA
                     TDSVIITGTSQFNNTVSDTGTYTTTAESAVFDINKYVIDTPTNPVPGDIITYKLTGHN
                     AGSAPTQDIRAEDIIPAGTTYVPGSMRFGPATATYDAATPLTDANDGYEVSGIGAYFD
                     GTKVVYVRDQFNAGDTGAFFFKVQVNSGALENTSISNFITAYYKHYGLPTEYSATSNT
                     TSTTIGLKASVDLDPNRTGTGNPGDQIVYSFTATNNGNAADRINITIPSSTMGWTWTI
                     WVDADSNGIPGTDGDYILTDTNGDGIIDTGILTPNGGHIQLLTVTTIPAGSSDQAIDV
                     TTIKGTSVRDGSVYDSITLTTTVTAPVLTITKSVSPTGNQPPGTILTYTITVTNTGTG
                     TATSVQIFDMIPAYTTYVPGSIETGPTVATLAPRTDGAEGDGGRYDSNANMVSAGSGT
                     SLNLGPGGTWVLRFKVTID"
     gene            2149..8265
                     /locus_tag="PLW07_01345"
     CDS             2149..8265
                     /locus_tag="PLW07_01345"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOL49201.1"
                     /translation="MKKILLKYKILAAVLCGIMFAISGNAVAFSPPPDTPIINTAIAK
                     YNDANGNPRSPVSTTVHTLISGAPVLVVDKIASSKVVSMGSTFTYTLNVTNTGNISVS
                     DITLVDYLSKHIVFVSASPEGMYHTGPPEGGFIEWNIPILLPGQTQTFTITARVKTTS
                     EYNAGDPETIGNGTIINNTAIASSASTTASTTLQITVGQNPNLEISQSVSSTVSSPGD
                     TLTYVIEYKNTGNSTATGVFITNPLPALTGVIPGTITNNGTIINNSIVWYIGDLQPQQ
                     QGSVSFSVTISPVAEYGSIITNNVSISSNQQGSIFAIPVSTLIAEGAASISITKNASS
                     STIIAGNNITYTIELENSGACPLTGVVVKDLLPEGTNFVSADSGGINIDGQVIWNTGT
                     LDISESKILTLVVSTNPSLNTQTLNNTAVVECSQLSPVTALSTITVNARTPGILTFLD
                     ENWSPKYSYKQGETLRIQVSDPDRNQDSILVETVVVILTNSITKDSETIVLTETGANT
                     GIFRTSIPTDTGPGIHNNGILSVASDSEVTATYSDPLDTIPVITKVAYIDPYGIVFNS
                     VTGTPLQGVIVTLIDAGTGQPAILPYDPNPAPATSSNGKYAFALVPAGTYYLKLTNLP
                     SGYSFPSVVPDHMLPAGYNVQTGSRGEHFILVSGMEPVNIDIPVDPPAGNLIVEKTVN
                     KNTASIGDIVKYTIKITNNGGAPVINVNICDTLPHGISYVKGSSTIDGSRLNDPEYQA
                     NRTIVWSIPIVAAGTTIELSYVAIIDIDSQKGDGKNTVFATGTSLGNQTVSNTVSATI
                     KISQAVFTSKGTIIGKVFVDRNNNAIQDPDEEGLEGVEIYMEDGTMVITDKEGKYSIP
                     LVEPGIHVLRINESTLPAGVETVPLNNKFSGTGSSQFVDVIKHGLSKANFAVRYKDRD
                     EEIYTIQIASYTRTGFTRAKELIEQLKDTGIEDIRLEKINDIFTVRAGVYKTKQEAEQ
                     TLEKLKKTLPDSFVKKAYYIEERIVQHQTGVSDKTQQASEKPIEWEEKIKELNNTLEF
                     LNPQDGTIIQANQTKVLLKAPMDTKISLLVNGSEIGDKQIGRKIQYEAGNIAIYEFVG
                     VKLARGEQNTLVANIMDDFGNIRQTQTITINVTGHAGKILVLPAKQTAYADGRSEIPV
                     EINILDKNDNIIAYSGAITVEVNGADIKEPDADKLTSGIQIPVSEGRAIFTVKAPYQP
                     VNAEIKVYADDILGTAIISFLPYLQNMFFVGIGELKIGSGNKKGSHYFDDKNFDDGLY
                     SSGKAALFLKGKIFNDMLLTASYDTGKTKNQDFFRESRQNTENDERYTVYGDESKINY
                     QALSTDKLYLRIDKGLSHAMYGDFKTNLKDAELAAYHRTFTGFSGDIQSGKFKIQSFV
                     SHSSRIQFVDTIRGKGISGYYYLSHVPVIEGSQKITIETRDRFRPDRVLKRETLIQGY
                     DYWIDYDTGAVLFKSPIPSMDADFNPVYIIAIYENETSGKKYYIYGGRISVKPVEQFE
                     IGVTDITEEKEVKNYHLTGIDATLNMPLNTTLKLEWAQSQSLFDIDHSLTPKTAEAFL
                     FRIKSSPLKNITLSAYYKKAGDYFDNISATDVMRGTEKYGADIRYTDGKNFSVFGKYF
                     NEKDTLNNGKYEHVSAGIEKKYEKTKFLIEAYYENSDNQYISQSSPDSRYPFDISQDL
                     PDQAAGFRLVFEKKLTPDLSLTGEYKNNLIATAGNMGQLGIEYQIDKNRKAYVREQFA
                     NLEGRSESYTVAGVEKQITQNTVAFNEYRIVNGMDGNAAQQSIGFRNSFMLGQNITGN
                     ISLENLHTVSGAERKDQPDAFATSLGFEYLPKDEVKITTRFEYRNAKHDISHLAEFGV
                     AYKLNPDYTLLFRERLFFNDLETGSKLTHRTIFGIAYRPISHDKFNMLGRIELKNDRD
                     TTSTPGYDTDAYIVSVEGIYQINRFTAITGKYAGKLAKDFGVSNYTDLVSARLTKDIG
                     SRFDMGIEYRVLCNHKTDTLLQGGSIEFGYNWFKNVWLSIGYCFDKFDTDLTGDSYSG
                     SGPFIRLRIKLDETSFKKSRAR"
     gene            complement(8266..9783)
                     /locus_tag="PLW07_01350"
     CDS             complement(8266..9783)
                     /locus_tag="PLW07_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HOL49202.1"
                     /translation="MVRKIGFDNEQYLKEQSEAILKRIDLFKGKLYLEFGGKLLYDYH
                     AARVLPGYDHNIKIKLLQQLKDKINILLCIYSGDIARGRIRGDFGITYDADALKLIDS
                     LRENGLDIHAVIITRFENQSPVIGFKNKLEHRGIKVYLHYATKGYPTDVNTIVSEEGY
                     GKNPYIETDKPVIVVTGPGPGSGKLATCLSQLYHDYNRGMKSGYAKFETFPIWNLPLK
                     HPVNIAYEAATADLGDINMIDPYHLNAYGESVVNYNRDIEIFPVLQRILKKIMGDNLP
                     YKSPTDMGVNRVGFAIVDDDVVKEAAKQEIIRRYFRYNYEYVMGIEPQQTFEKIILLM
                     EELNLKPEDRAVVLPARMAAERAKGKISGNFYECGAAIETPDGNIITGHNSVLMTAPS
                     SVVLNAIKHMAGIPDKIHLLSSSIIEHIKSLKRDCLELKHEIMDLQETLIALSISATN
                     NPAAEMAMEKLRDLQGCEVHLTSIPSPGDEIGLRRLKVNLTADASFPSKDLFLDH"
     gene            9943..10446
                     /locus_tag="PLW07_01355"
     CDS             9943..10446
                     /locus_tag="PLW07_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49203.1"
                     /translation="MRKKVVVNDKMQKNYVYHLTEEMGKNFDPEFKPELTPKEMLELG
                     VFGGKYMTDCKNEFPSDWFEKAKLSPDKRNPKLNYFGVNASQPLSVWRKKGWIHPDDP
                     RGWFQWYCRYYMGRRHEDDKRQIKRWKAIKRHIGQIKKHCRKGDINCRKKQRQAILHW
                     AYDSRKI"
BASE COUNT         3658 a         2190 c         2097 g         2879 t
ORIGIN      
        1 atcgttaacc cgatacccaa tggcacaacc tatatattaa acagcgccag tggcaaggat
       61 actgaaattt cattcagtat tgatggcggg aaatattttc aaaagccacc cgttaagtat
      121 cttgtgaaaa aacctgatgg ctcaacagaa gaaaaaattg caacaccaga aatgtacacg
      181 catatcatgt ggacaattaa aaaacctata cctgcaggag cgtcaggtga agtttctttc
      241 agtgtaaagg taaagtgaaa atcgttggaa aattttacaa attccttaaa agggaaagga
      301 gggaaggaca atggaaggga taaaaagaaa aaccctaaca aaaaaaatga aaggaggtgg
      361 aaaaatgaaa aaattgcttt tattagcagt agggctcctg gcagcaggca tgttcatcgc
      421 gccgtctgca atggcacttg gtacacctgc aggcacagtg atatcaaacc aggcatacgt
      481 tgattacaag gatgcaaatg gaaacccgaa aacacgcgta tattcaaaca cagttacaac
      541 tgtggtcagc caggttgctg gagtatccat tgaacctgca accgctgcaa agtcagggct
      601 tgctggtgga acagtgtact atggagtgac tgtatgcaac acaggcaatg gtcctgatac
      661 catcaatctt gcgctttcag gtaacacatg ggctgctgtc atttattttg acaccaatgg
      721 agatggaata tgggaccctg tcaatgaaac aaccactgta acaaatactg gtgtacttgc
      781 agcagacgcc tgctataagg tgctggtggt tgtaacaatt ccttctggtg tagcaaatgg
      841 agcaactgac agcgtaatta ttacaggaac ttcgcaattc aacaatactg tctcagacac
      901 agggacttat accaccacag cagaatcagc agtgtttgat atcaacaaat atgtcataga
      961 tactcccaca aatccagttc caggcgatat catcacctac aaacttaccg gtcacaatgc
     1021 tggaagtgcg ccaactcagg atatcagggc tgaggatatt attccagcag gcaccacata
     1081 tgtacctgga agtatgaggt ttggtcctgc cacagcaacc tatgatgcgg cgacaccatt
     1141 gacagacgct aatgatggat atgaagtatc aggcattggc gcatattttg atggaacaaa
     1201 ggttgtttat gtcagagacc aattcaatgc aggtgatact ggagcatttt tcttcaaggt
     1261 acaggtcaat agtggagccc ttgaaaacac ttctatctca aactttatta ctgcttatta
     1321 caaacactat ggacttccca cagaatactc agccacaagt aatacaacca gcacaaccat
     1381 tggattaaaa gcatcagttg accttgatcc gaatagaacc ggtacaggaa atccaggaga
     1441 ccagattgtc tattcgttta ctgcaaccaa caacggaaat gctgctgaca gaatcaacat
     1501 cacgatacca tcctcaacaa tgggatggac atggacaata tgggtggatg ctgacagtaa
     1561 tggaattcca ggaactgatg gtgactatat ccttacagat acaaatggag acggcatcat
     1621 tgacactggt atcctgacac caaatggagg acacattcaa ctgcttactg tcaccacaat
     1681 accagcaggt tcatcagacc aggcaatcga tgtcacaacc atcaaaggaa catctgtaag
     1741 agatggctct gtatatgata gcatcacact cacaacaacc gtaacagcac ctgtgttaac
     1801 tatcacaaaa agtgtaagtc caacagggaa ccagcctcct ggaacaatcc ttacctacac
     1861 cattactgtt accaacacgg gtacaggaac agcaacttct gttcagattt ttgatatgat
     1921 tcctgcctac acaacgtatg ttccaggttc cattgaaact ggtccgacag tggcaaccct
     1981 tgcgccaaga acagatggtg ctgagggaga tggaggaaga tacgattcca atgcgaacat
     2041 ggtttccgca gggtcaggaa cttcattaaa ccttggacca ggtggcacgt gggtactcag
     2101 attcaaggta acaattgact gacaggcaag aacaataagg tgataggagt gaaaaaaata
     2161 ctgttaaagt ataaaatact ggcggcggtg ctgtgtggca taatgtttgc cataagtggt
     2221 aatgccgttg catttagccc gccgccagat actcctatta tcaacactgc gattgcaaaa
     2281 tacaatgacg caaatggaaa tccgcgttca cctgtcagca caacagttca cacacttatt
     2341 tcaggcgctc cggtactggt ggtagacaaa attgcaagca gcaaggttgt gtcaatgggt
     2401 agtacattca catacacatt gaatgttaca aatactggca atatcagtgt ttctgatatt
     2461 acccttgtag attatctttc aaaacatatt gtttttgtat ccgcatctcc tgaaggtatg
     2521 tatcacacag gtccgcctga aggcgggttt attgagtgga atattccgat tctcctgcca
     2581 ggacaaacac aaacgttcac aataacggca agggtaaaaa caacatctga atataatgca
     2641 ggagaccctg aaacaatagg aaatggaacc atcattaaca atacagccat tgcatcgtct
     2701 gccagtacta cagcaagtac aaccctgcaa ataactgtag ggcagaatcc gaatcttgaa
     2761 atttctcaaa gcgtttcatc aaccgtatca tctcctggag acacacttac ctatgtcatt
     2821 gaatataaaa atactggaaa ttctactgca acaggcgtat ttatcaccaa tcctttgcca
     2881 gcactcacag gtgttattcc aggaaccatc acaaataatg gcacaattat caataattca
     2941 atcgtctggt atattggaga tcttcagcca caacaacagg gaagtgtttc attcagtgta
     3001 acaatctcac cagtcgcaga atacggaagc attatcacaa acaatgtttc aatttccagc
     3061 aatcaacagg gaagtatttt cgccatacct gtttctaccc ttattgcaga aggcgctgcg
     3121 tctatatcaa taacaaaaaa tgcaagttca tcaacaataa tagcaggaaa caatattaca
     3181 tacaccattg aacttgaaaa cagtggagca tgcccgctta caggtgtggt ggtaaaagat
     3241 ttattgcctg aaggaacaaa ttttgtcagc gctgattcag gtggcatcaa tattgatggt
     3301 caggtaatct ggaatacagg cactcttgat atttctgaat caaaaattct gaccttggtt
     3361 gtatccacaa atccttcatt gaatacacaa acgttaaata atactgccgt tgtggaatgc
     3421 tctcaacttt ctccagtaac tgctttgtca acaatcactg taaatgccag aactcctggc
     3481 atccttacat tccttgatga aaactggagt cctaaatatt cttataaaca gggagaaacg
     3541 ttacgcattc aagtatcaga ccctgacaga aaccaggatt caattcttgt agaaactgtt
     3601 gttgtcatcc ttaccaattc catcaccaaa gattcagaaa ccattgtcct tactgaaaca
     3661 ggcgccaata caggcatatt caggacatca attccaacag atactggtcc tggcatacat
     3721 aacaacggca tactcagtgt cgcttctgat tctgaggtaa cagcaacata ttcagacccc
     3781 cttgatacca ttcctgtgat cacgaaagtt gcttatattg acccgtatgg aattgtattt
     3841 aactcagtca caggaacacc actgcagggc gtgattgtca cattaataga cgcaggcaca
     3901 ggacaacctg ctattctccc atatgacccg aatcctgcgc ctgcaacctc atcaaacggc
     3961 aaatacgcgt tcgcccttgt tcctgctgga acatactatc taaagttaac aaaccttcct
     4021 tctggttaca gtttcccatc tgttgtacca gaccatatgt tacctgcagg atacaatgta
     4081 cagacaggat caagaggcga gcattttatc cttgtttctg gaatggaacc agtaaatatt
     4141 gatatcccgg ttgaccctcc tgctggaaac ctcatcgttg aaaaaactgt gaataaaaat
     4201 actgcgagta ttggcgacat tgtgaaatat acaatcaaaa tcacaaacaa tggcggcgcg
     4261 cctgtaatca atgtaaacat ttgtgatacc ctgccccatg gtatcagtta tgtcaaaggg
     4321 tcttctacca ttgatggcag tcgtttgaat gaccctgaat atcaggcaaa tagaacaata
     4381 gtatggtcaa taccaatagt tgcagcagga acaacaatag aactttcata tgtagcaatc
     4441 attgatatag acagtcagaa aggagatggg aaaaacacag tatttgccac tggaacaagt
     4501 ttgggaaatc aaactgtttc aaatactgtt tcagcaacaa tcaaaatttc acaggccgtg
     4561 ttcacctcaa aaggcacaat tataggaaaa gtatttgttg acagaaataa taacgcaatc
     4621 caggaccctg acgaagaagg attggagggt gtggaaatat acatggaaga tggaacaatg
     4681 gtgattactg acaaagaagg gaaatattcc attccactgg ttgaaccagg aatccatgta
     4741 ttgagaatca atgaatcaac attgcctgct ggcgttgaaa ctgtgccact gaacaacaag
     4801 ttttctggta ctggttcatc ccagtttgtg gatgtcataa aacacggtct ttccaaagca
     4861 aattttgcag tgagatataa ggacagagac gaagaaattt acacaattca aatagcatca
     4921 tacaccagga caggtttcac cagagcaaaa gaattgattg aacaactgaa agatacagga
     4981 attgaagata tcagattgga aaaaatcaat gatatattca ctgtccgcgc aggcgtgtac
     5041 aaaacaaaac aagaggcaga acagacactt gaaaaactga aaaaaacctt gccagatagt
     5101 tttgtgaaaa aggcatatta cattgaagaa agaatcgttc aacaccaaac aggcgtttct
     5161 gacaaaacac aacaggcatc tgaaaaaccc attgaatggg aagaaaaaat aaaagaactt
     5221 aacaatactc tggaattttt gaatccccag gatggcacaa tcattcaggc aaaccagaca
     5281 aaagttttgt taaaggcacc catggataca aaaatttcac ttttagtcaa tggttcagaa
     5341 attggcgata aacaaatagg cagaaaaatc caatatgaag ccggaaacat cgccatttat
     5401 gaattcgttg gcgtaaaact tgcccgtgga gaacaaaaca cacttgttgc caatatcatg
     5461 gatgattttg gaaacatcag acagacacaa acaatcacca tcaatgtcac aggacatgct
     5521 gggaaaatcc tggtactacc tgcaaaacaa acagcatacg cggatggaag gagcgaaata
     5581 cctgttgaaa ttaacatcct tgataagaat gacaatatca ttgcgtattc aggtgccatc
     5641 actgtggaag taaatggagc agacataaaa gaacccgatg cagacaaatt gacttcaggc
     5701 attcaaattc ctgtatctga aggcagggca atcttcactg tcaaagcacc atatcaacca
     5761 gttaatgcag aaataaaggt ctatgctgac gatattctgg gaacagccat tatcagtttc
     5821 ttaccgtatc tgcagaacat gttctttgtt ggaatcggcg aattgaaaat aggaagtgga
     5881 aacaagaaag gttctcatta ttttgacgac aaaaattttg atgatggtct ctactcatcc
     5941 ggaaaagcag cgttgttcct gaaaggaaaa atatttaatg acatgcttct gacagcatca
     6001 tatgatactg ggaaaacaaa aaaccaggat ttcttcagag agagcagaca gaatacagag
     6061 aatgacgaaa gatacacagt atatggagat gaaagcaaaa tcaattatca ggcgttatca
     6121 acagacaaac tttatttgag aattgacaaa ggcctttctc atgcgatgta tggtgatttt
     6181 aaaaccaact taaaagatgc agaacttgca gcatatcaca gaacattcac tggtttcagt
     6241 ggcgacatcc agtcaggcaa attcaaaatc cagtcctttg tgagccattc ttctcgcatt
     6301 cagtttgttg acacaatacg gggaaaggga atttctggat attattatct ttcacatgta
     6361 cctgtcattg aaggaagcca gaaaattaca attgaaacaa gggacaggtt cagacctgat
     6421 agggtgttga aaagagaaac cctgatacag ggatatgact actggattga ctatgacacg
     6481 ggtgctgtgc tgttcaaatc cccgattcct tcaatggatg cagatttcaa cccagtatat
     6541 atcattgcca tttacgaaaa tgagacatca ggtaaaaaat actatattta tggaggaaga
     6601 atatcagtca aaccagttga acaatttgaa attggtgtca ctgacatcac agaggaaaaa
     6661 gaagtgaaaa actatcatct tactggcatt gatgcaacat tgaatatgcc tctgaatacc
     6721 acattaaagt tagaatgggc gcaaagtcag tcattgtttg acattgacca ctctcttact
     6781 ccaaaaacag cagaagcatt tctgttcaga ataaaaagca gtccattgaa aaatattacc
     6841 ctgagtgctt attacaaaaa agcaggggat tactttgaca acatttctgc aacagatgtg
     6901 atgaggggca cagaaaaata tggagcagac attcgttata ctgacggtaa aaacttttca
     6961 gttttcggca aatatttcaa tgaaaaggat accctcaaca atggcaagta tgaacatgtt
     7021 tcagcaggta ttgagaaaaa atatgaaaaa acaaagttct taattgaagc atactacgaa
     7081 aattcagata atcaatacat ttcacaatca tcaccagatt caagatatcc ttttgatatc
     7141 agccaggacc ttccagacca ggcagcagga ttcagattgg tatttgagaa aaaattaacg
     7201 cctgatttgt cccttacagg agaatacaaa aataatctta ttgctacagc aggaaacatg
     7261 ggacaactcg ggatagaata tcagattgat aaaaacagaa aggcgtatgt cagggaacaa
     7321 ttcgctaatc tggaaggcag gtctgaatca tatacagtgg caggcgttga aaaacagatt
     7381 acgcaaaata ctgttgcgtt caatgaatac agaattgtta atggaatgga cggaaatgcg
     7441 gcgcaacaaa gtatcggttt tcgcaatagt ttcatgctcg gtcagaatat cactggaaac
     7501 atttctcttg aaaatctcca tacagtaagc ggcgctgagc gaaaggacca gcccgacgca
     7561 tttgccacca gtttaggttt tgagtacctg ccaaaagatg aggtaaaaat tactaccaga
     7621 tttgaataca gaaacgcaaa acacgatatt tcccatcttg ctgaatttgg tgttgcatat
     7681 aaattgaatc ctgattacac tttgcttttc agagaacgat tattctttaa tgaccttgaa
     7741 acaggatcaa aactcactca cagaactata tttggaattg cttacaggcc catttcacat
     7801 gataaattca atatgcttgg acggattgaa ctgaaaaatg acagggatac cacatcaacc
     7861 ccgggttatg atactgacgc atacatcgta tctgttgagg gcatttacca gattaacaga
     7921 ttcactgcta tcactggaaa atacgcagga aaacttgcaa aggatttcgg agttagcaat
     7981 tatactgacc ttgtatcagc aagattaaca aaagatattg gcagcaggtt tgatatgggt
     8041 attgaataca gagtactttg taatcacaaa accgacactc tcctgcaggg cggttccatt
     8101 gaatttggat ataattggtt taaaaatgtg tggctgtcaa tcggttattg ctttgataaa
     8161 tttgatactg atttaactgg cgacagttac agtggtagtg gtcctttcat tcgattgaga
     8221 ataaaactgg acgaaaccag tttcaaaaaa tccagagcca gataatcaat ggtccagaaa
     8281 caaatctttt gaagggaaac ttgcgtctgc agtcaaattg acttttaacc tgcgcaatcc
     8341 gatttcgtct ccaggtgaag gaatactggt cagatgcacc tcacaaccct gcaaatccct
     8401 aagtttttcc atagccattt cagcagcagg attgtttgtt gcagatatac tgagggcaat
     8461 aagggtttcc tggaggtcca taatttcatg tttcaattca aggcaatctc ttttcaaact
     8521 ttttatgtgt tctataattg aagaagaaag caaatgtatt ttatcaggta ttcctgccat
     8581 atgcttgata gcattcagaa caacacttga aggagcagtc atcaacactg aattatgtcc
     8641 ggtaataata ttgccatcag gcgtttcaat ggcagccccg cattcataaa aatttcctga
     8701 tatcttccct tttgcccttt ctgccgccat gcgggctggc aataccacag ccctatcttc
     8761 tggtttaaga tttaattctt ccatcaagag aataatcttt tcaaatgttt gctgcggctc
     8821 aattcccatc acatattcat aattgtagcg aaaatatctt ctgataatct cctgttttgc
     8881 tgcttctttt accacatcat cgtcaacaat tgcaaatccc actctgttaa cacccatatc
     8941 tgtgggagat ttatatggta gattatcgcc catgattttt ttcaaaatcc tttgtaatac
     9001 cggaaatatc tctatatccc tgttgtaatt gacaacactt tcaccataag cattgagatg
     9061 atagggatca atcatattta tatcaccaag gtcagctgtg gcagcttcat aggcaatatt
     9121 gacaggatgt ttcaaaggaa gattccagat aggaaatgtt tcaaacttgg cgtatcctga
     9181 tttcattccc ctattgtaat catgataaag ttgcgacagg catgtggcta attttccgct
     9241 gccaggacct ggtccagtca ccacaataac gggtttatct gtttcaatgt atgggttttt
     9301 cccatatcct tcttcgctca caatggtgtt gacatctgtt ggatagccct ttgtagcata
     9361 atgaagatat acctttattc ccctgtgttc cagtttattt ttaaaaccaa tcacaggtga
     9421 ttgattttca aaacgggtga taatcactgc gtgaatatca agaccgtttt ctcttaaact
     9481 atcaatcagt tttagggcat ctgcgtcata agtgattcca aaatccccac gaatccttcc
     9541 ccttgcaata tcgccagaat aaatgcataa aagaatatta attttgtcct tcaactgttg
     9601 aagcagtttt atttttatat tgtggtcata accaggaagt actcttgcgg catgataatc
     9661 gtaaagcagt tttccgccaa attcaagata cagtttcccc ttaaaaaggt ctattctctt
     9721 aaggattgcc tcgctttgtt ctttcagata ttgttcgttg tcaaaaccga tttttctgac
     9781 catctataaa ctcctgcgag aaaattccgt ataatgttaa catcagaaca taaaaattca
     9841 aaatcgcatc ttaaggatct tgacaatttt ttaacattta aacaatcttg cattaaaata
     9901 ttctcaaata actgaacaga tcttattaat tattgataaa aaatgagaaa aaaagttgtg
     9961 gttaatgata aaatgcagaa aaactatgtg tatcatttga cagaggaaat gggtaaaaac
    10021 tttgaccctg aatttaaacc agaattaacc cccaaagaaa tgcttgaact tggtgtattt
    10081 ggtggtaagt acatgactga ttgcaaaaac gaattcccat ctgactggtt tgaaaaagca
    10141 aaactttccc ctgataaaag gaacccaaaa ctcaactatt ttggagtcaa tgccagccag
    10201 cccttatctg tatggagaaa aaaaggatgg attcatcctg atgacccgag aggatggttt
    10261 caatggtact gcaggtatta tatgggaaga cgccacgagg atgacaaaag acagattaaa
    10321 agatggaaag caattaaaag acacatcggc cagataaaaa aacactgtcg caaaggcgac
    10381 atcaattgta gaaaaaaaca aagacaagca attttgcatt gggcttatga cagcagaaaa
    10441 atataataaa ttttacacaa attttaatga atataggcca gcatcctgca ttatgagaat
    10501 ttcaattgtg attttaaatt aacccaaatt ttgtttaatc tccagcacaa tctttcttat
    10561 attaaattcc catcacaact tcctgcagga tgggacgcag aagtattata ctactcccta
    10621 ttaagaagga atgggaaata atatactgct ccccattaag gggaaatgga aaataagata
    10681 gaatatagtg gtctaaaagg gaagcaggat aacatcacaa ccttttttca tactatttcc
    10741 catctttata aaattacctc atactacccc ccctctttat aaaagagggg ctgggggaga
    10801 tttctaaatc cccctcaatc cccc
//
LOCUS       DAORYU010000022        10699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_4681_length_10699_cov_5.016347, whole genome shotgun sequence.
ACCESSION   DAORYU010000022 DAORYU010000000
VERSION     DAORYU010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10699
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4681_length_10699_cov_5.016347"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="PLW07_01360"
     CDS             <1..753
                     /locus_tag="PLW07_01360"
                     /inference="COORDINATES: protein motif:HMM:NF018647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOL49204.1"
                     /translation="HLKKMEDDIISLSEKKTSRRIGIALSEWGIWYRRGHKDALDEVY
                     NLKDALVFASAMNLLLGFCNSLDFTHQSMLVNCLGLIRTKQNKIVRQTIYFPFLLFAN
                     ETGDTVLAPLVLCETFSCKDYRYFPWPTFDVGKDNFQMDESRVPQMHNIQFLDVSATF
                     NSKENYLCIIAVNRHPEQSISSSIELRNCQYKNLVTASVITGPDVYAENNFDKEAVGM
                     ETYQETLKNHLVWDFPPHSITMLKIPVEAKNE"
     gene            746..1933
                     /locus_tag="PLW07_01365"
     CDS             746..1933
                     /locus_tag="PLW07_01365"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOL49205.1"
                     /translation="MNKLNVLVIGGGMITQQVILPLLIEQKEKGKISSIAVASRRKST
                     IEQLSALFPQAKIKGYPEKKDQDPDGWKIAIKELEKPGVVIVATPDDLHAEMTLAAIE
                     QHHDVIVQKPLCLTVGDAKNILDQSRKNAVYVFTDYHKRHDLSLRAAQYKYRKGELGE
                     VLCGHAWHEQRREIPLKYFRNWCERSSPLEYLGTHYIDMFYFITNLTPQKVIARGQKK
                     LLVSLGINAYDACQAFITWSNGAIQYLQTSWILPESNPCLSNQGFQITCTEGEFRADN
                     ANRNSNFSTTKNGYELFNPYFFKPYLDWNDFEKTTYLGYGADSIIQAIDDCLHIVEKT
                     LHLDQKQAFEKRKELISQFEKTRPLPNQAIIATSVIEAGKMSMIEDGKTVVIDKDFSL
                     HYE"
     gene            complement(1924..3126)
                     /locus_tag="PLW07_01370"
     CDS             complement(1924..3126)
                     /locus_tag="PLW07_01370"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOL49206.1"
                     /translation="MTPRERVLNAIRHQPVDKIPVDLGGMDSTGITAVAYNKLKKFLG
                     ITSGKTRVYDPYQQLAIVEQEILNIIGADVLPVFPEPGRWKSAKLPDGSLCEIPARWN
                     PLQLEDGSEHVLDETGKVIAKRPENCWYFEVVDFPLAQAQSIRDIESRRDIFENFDWP
                     FFVDETIEDLGKKAKLLYETTDYALMGNFAVHVFMGAQLLRGFEQFMMDLVLQPEIAE
                     CIFDHLVNAYIERFKKYRKHVCDYVQIVNVNDDLGTQQGLQISPVMYRKLVKPYQKKL
                     YQFIKESNVFLFLHSDGSIYDIIPDLIEIGVDIINPVQFTAENMQLEKLKKEFGKYIT
                     FWGGGCDTQHILPYAEMQQVKTHVQQCVKILSQGSGFVFCQIHNIQPDVPPENIMAMY
                     EAVHDFHS"
     gene            complement(3123..4325)
                     /locus_tag="PLW07_01375"
     CDS             complement(3123..4325)
                     /locus_tag="PLW07_01375"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOL49207.1"
                     /translation="MLKVGIIGFGLRVSHMAAAMKIFDVPYKIAAIADPAAEKIEKSV
                     KENIPEIHSHRRIDPDLMKDTRYFTSADEMLEKIELDGVMIGTRCYLHTEMACKVAKK
                     NLPLFLEKPVAITFDQVKMLEKAFKNVTAPTVISFPLRLTPMAQKVKQIIDSGRIGEV
                     EHVVAWNDVPYGSVYFNSWYRNYHEVGGLFLQKATHDFDYIFYLTGRKPKMIAAMNSQ
                     RIYGKRNKPFELLCRDCDEKMECIESPFNLFYKRFKGEKVEWNDWQKCMFSKEIKNED
                     SGNALIELDNGVQVSYSQNFFVRNQAVGRRGARLYGYKGTIDFDWYRNNIIIYDHFTP
                     IVEKIDFTGDMPHFGGDRELVYDFLMAMKEKRPSRSPIEAGITSALTCLWARESAEKR
                     QFFEVKMP"
     gene            4424..5203
                     /locus_tag="PLW07_01380"
     CDS             4424..5203
                     /locus_tag="PLW07_01380"
                     /inference="COORDINATES: protein motif:HMM:NF018290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOL49208.1"
                     /translation="MAAIYSITNQKGGVGKTTTCLCLGSALAEKGYKVLLIDFDPQSG
                     LTVCLGFDPDAFSTTIYNVIINCEQYPLQEIITDTKVPNLKIAPANLDLAGAEGELIG
                     EIGWDRNLRDAINPIKNEFDFIFVDCPPSLGVLTTNALISAQTVIVPVQTEFLALRGL
                     KQLGDIITKVRKKGNPSLKKRILRTMHDVRTLHSKEVVEELERIFPDEIFNTIIKRTV
                     KFADASLAGVPILLYAKSSEAAEAYRQLAEEVIEDAKKTIS"
     gene            5181..5468
                     /locus_tag="PLW07_01385"
     CDS             5181..5468
                     /locus_tag="PLW07_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49209.1"
                     /translation="MPKRPSLKGRGVDIFLGKSDQKEKKPVKEEKKGKATFYFPQKLL
                     SELDEAWFILRRIDRKIKKSDIVQAALEISIQGLKEGKKENPLIEYFKKEK"
     gene            5539..6108
                     /locus_tag="PLW07_01390"
     CDS             5539..6108
                     /locus_tag="PLW07_01390"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOL49210.1"
                     /translation="MNEFDDETLVRRVQEENDMYAFEMIVTKYQNKVYGICFRFMGEH
                     HEAFDCAQDTFVKIYQNIRSFKHLSSFGTWVYRIAVNTCKNRLSSWDFVRKKRTTYLG
                     NGCTDIENQCVSPAQQYKKTTIVEIVQNAINKLPFDQKQIVILRDIQQLSYEEIEKIT
                     GLPLGTVKSKLSRARRKLKELLKDVEHEL"
     gene            6098..7039
                     /locus_tag="PLW07_01395"
     CDS             6098..7039
                     /locus_tag="PLW07_01395"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOL49211.1"
                     /translation="MNCKQVQQILSDYLEGFVSDLQKKEIEQHLQQCEQCQIELDELK
                     KTVELLKNLPAPPVSEKLVESVMEKISTTGIRKRKFTLLLTGIACAAIIAFVVLFKLS
                     QPVKIAYQPETKMKSAQTTVEEKKTEITPKTPVSREKKIAVISSKETEKQEKTEIVIQ
                     LAYLGQPQKVYEKRNSIKEKPTDIAMDTMETAQQKEIIMREPRIEKAESEDKKSQEIA
                     KPLAARPVIKPEPVEQLSTESEIIKHIHAAGGKILSESPVSDTEISHVFVAEIPGSMY
                     KNFLDHLAGTFQIKNYDSIKDVTISETFITIRLQLFR"
     gene            complement(7032..7652)
                     /locus_tag="PLW07_01400"
     CDS             complement(7032..7652)
                     /locus_tag="PLW07_01400"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOL49212.1"
                     /translation="MNLFAIGLSSFIVALSGALSPGPLFAVTITRSLKYGARQGPLLI
                     AGHSILEFLMVVLLVAGFGTYLKQPGVSRFLSVAGGCVLVLMAGLMLKKTKTDLPIEK
                     NVNTTKTLSVINGMVVSVSNPYWTIWWLTIGLTYLSIALPRGFAGLTAFFLGHISADF
                     FWYTLVSYSISKGKTKFTPPVYRIVTVCCGIFLSLFGVFLIIKAFI"
     gene            complement(7652..8449)
                     /locus_tag="PLW07_01405"
     CDS             complement(7652..8449)
                     /locus_tag="PLW07_01405"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOL49213.1"
                     /translation="MKFAFMSFSCPEADLYKMLDYARKYGYDGVEPRAQAQHNHGIEI
                     EASKEKRKEIKKFFDESGIECSCIATSIRYCFTDAQKRKESIGMTRAFIDLACDIGVK
                     RIRVFGGVPDKEISVDEAIKIVGECLGIAGEYAEKNRVFICLETHDFFSRADTVAKAV
                     KVAGSPNVKINWDIMHPYTQGMTIKEAFDIVKEFVEHCHIHDGIYDQDRKVTLALMGQ
                     GEIPYKTALRLLQEINYQGFFSGEYINAWEPDVVLPHDIKVLKSYLL"
     gene            complement(8469..>10699)
                     /locus_tag="PLW07_01410"
     CDS             complement(8469..>10699)
                     /locus_tag="PLW07_01410"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOL49214.1"
                     /translation="AWVEHCWIHSDPVGKCMDFMIKTGGNQEDVDLEVFVKDPSGKQC
                     GQITRTSGHGKIVFSIPVKEGRLWTPETPFLYQVDVELKRNEEIVDCICERVGFLHLS
                     TKDKHFLINNQPYYMMGTGDFSVNPETGCPDTDRQRWRKKLSVLRQYGYIYIRCQSYV
                     PVPEYLDVADEVGLLVQSEIGMMGAWGGHSQYHQYQWPQPDAERRELLRYQWNAAVIR
                     DVNHVSANLYCMSNEYGHPVILYPETAWKCYRETKAIKPSSFVIWTDGTVRKIKGMPL
                     DFYCAEAKEDNRVSMPVIQHEFRWWTSFPDISNKGKFTGAVRPYAIEIALENAKKAQL
                     DHLLPELITNSQKLQFIEAKTKLEQIRRDYPNLAGICHFTAMDIGFSPQGVIDEFYEK
                     KFVTAEVWRQTTGPTAILTDSNFDDRIYVSGMDFEKTIFISDFSHPPLKNPAIEWDFS
                     GETVKEHGEFSFTHKPFCTDKIGNIRIRLPDVQSPEKVRLRIKAKEEGRTFENHWDYW
                     IFPETTNMVEFVVYGKAKKTWIKTLKAQNIKKSYIRGVFPKLVVSEIFDRNLANYVAD
                     GGKLILAVPEGFTRPFFPKLGLVAGRYFFTPPANYPTYEDGNTGTIVRNHPMLGDFPH
                     ENFCDLQFYRMIAEFPPIDLRPFGDLKEEPVIRSFSTCFRMYPLAYLIEFSWGKGHII
                     ITSLNFDQNLPEARYLFTQMLKYLENAKLPENKLNDVVVDRLIEESSEKIDIFGSLKL
                     KE"
BASE COUNT         3417 a         2187 c         1909 g         3186 t
ORIGIN      
        1 catttgaaaa aaatggaaga cgatattatc tcattgtcag agaaaaaaac atcaagaaga
       61 attggcattg ctctttctga gtggggtatc tggtacagac gcgggcataa ggatgccctt
      121 gatgaagtat ataacctcaa ggatgccctt gtttttgcaa gtgcaatgaa ccttttgctc
      181 ggtttttgta attcccttga tttcacacat cagtcaatgc ttgtaaactg ccttggtctt
      241 atcagaacaa aacagaataa aattgtaaga cagacaattt attttccttt tcttctcttt
      301 gcaaatgaaa caggagatac agtacttgct ccactggtac tgtgtgaaac tttttcctgc
      361 aaggactaca ggtatttccc ctggccaact tttgatgtag gaaaagataa tttccagatg
      421 gatgaaagcc gtgttccgca gatgcataac attcagtttc ttgatgtttc tgcaacattc
      481 aactcaaaag agaattacct ctgtatcatt gctgtaaaca ggcatcctga acagtcaatt
      541 tcaagttcaa ttgaacttcg caattgtcaa tataaaaacc ttgtcactgc atcagtgatt
      601 acaggccctg atgtatatgc agaaaataac tttgataaag aggcggttgg aatggaaaca
      661 taccaggaaa cattgaaaaa tcatctggta tgggatttcc cacctcactc aatcacaatg
      721 ctgaaaattc ctgtggaggc aaaaaatgaa taaactgaat gttcttgtca ttggcggtgg
      781 aatgattaca caacaggtga ttttgccttt gctgatagag caaaaagaaa aaggaaaaat
      841 ttcatccatt gctgtggcat caagaagaaa aagcacaatt gaacaattgt cagcactttt
      901 tccacaggca aaaattaaag gatatcctga aaaaaaagac caggaccctg atggatggaa
      961 aattgccata aaagaactgg aaaaacctgg cgttgtaata gtggcaacgc ctgatgacct
     1021 ccatgcagaa atgacacttg ctgcaataga acaacaccat gatgtcattg ttcaaaaacc
     1081 tttatgcctc actgtagggg atgcaaaaaa tatcctggac caatcaagaa aaaatgcggt
     1141 gtatgtattc actgattacc acaaaagaca tgacctgtcc cttcgcgcag cccagtacaa
     1201 atatagaaag ggtgaacttg gagaagtgtt atgtggccat gcctggcatg agcagagaag
     1261 agaaattcca ttaaaatact tcagaaactg gtgtgaaaga agcagtcccc ttgaatatct
     1321 tggtacccat tacatagaca tgttttattt cattacaaat ctaactccac aaaaagtaat
     1381 tgcaaggggg cagaaaaaac ttcttgtttc tcttggaatc aatgcgtatg atgcatgcca
     1441 ggcatttatc acctggagta atggtgctat tcagtatctt cagacctcat ggatattgcc
     1501 agaatccaat ccatgcctgt caaaccaggg atttcagata acctgcacag aaggggaatt
     1561 cagggcagac aatgcaaaca ggaattcaaa tttctcaaca acaaaaaatg gctacgagtt
     1621 attcaacccg tattttttca aaccctatct tgactggaat gattttgaaa agacaacgta
     1681 cttgggatat ggtgctgata gcatcattca ggcaattgat gattgtctgc acattgtgga
     1741 aaaaacattg catcttgacc agaaacaggc atttgaaaaa agaaaagaac tcatcagtca
     1801 atttgaaaaa acaagacccc tgcccaatca ggcaattatt gcgacaagtg tcattgaagc
     1861 aggaaaaatg agtatgattg aggatggaaa aactgttgtc atcgataagg atttttctct
     1921 tcattatgaa tgaaaatcgt gtactgcttc atacattgcc ataatgtttt caggcggtac
     1981 atctggctga atattatgaa tctggcaaaa aacaaaaccg cttccctgag aaagaatctt
     2041 cacacactgt tgaacatgtg ttttcacctg ttgcatttca gcatatggca aaatgtgctg
     2101 ggtatcacaa ccgccacccc agaatgtaat atacttgccg aactcttttt ttaatttttc
     2161 aagttgcatg ttttcagcag taaattgcac aggattgatg atgtcaacgc caatttcaat
     2221 caaatcagga ataatgtcat agatggaacc atctgaatga aggaatagaa aaacattgga
     2281 ttctttgata aactgataaa gttttttctg atagggttta acaagttttc tgtacatcac
     2341 aggtgaaatt tgaagtccct gctgggttcc taaatcatca ttaacattga caatctgcac
     2401 ataatcgcac acatgctttc tgtatttttt gaatcgttca atatacgcgt ttacaagatg
     2461 atcgaaaata cattctgcaa tttctggttg cagaacaagg tccatcataa actgttcaaa
     2521 acccctcaat aattgcgctc ccataaacac atggacagca aaatttccca tcagtgcata
     2581 atctgttgtt tcgtaaagaa gttttgcttt ttttccgagg tcttctattg tttcatccac
     2641 aaaaaaaggc cagtcaaaat tttcaaaaat atcccttctt gattcaatgt ctcttattga
     2701 ttgtgcctgt gccaggggaa aatcaacaac ttcaaaatac cagcagtttt ctggcctttt
     2761 tgcaataacc ttcccggtct catccagtac atgctctgaa ccatcttcaa gttgcaatgg
     2821 gttccatcgg gctgggattt cacacagaga cccgtcagga agtttcgcac ttttccatct
     2881 tcctggttca ggaaacacag gcaatacatc ggctccaata atgtttagta tttcttgttc
     2941 aacaatcgca agttgctgat atgggtcata aacccttgtc tttcctgatg ttattccaag
     3001 aaattttttc agtttgttgt aagcaacagc cgtaatacca gttgaatcca tcccaccaag
     3061 gtcaactgga attttgtcca ctggttgatg tctaattgca ttcagaactc tttctcgtgg
     3121 tgtcatggca tttttacttc aaaaaattgt cttttttctg ctgattctct tgcccacaga
     3181 catgtaaggg cgcttgtaat ccctgcctct attggtgaac gagacggtct tttttctttc
     3241 attgccatca agaaatcata gacaagttct ctatcaccgc caaaatgtgg catgtctcca
     3301 gtaaaatcaa ttttctccac aataggcgta aaatggtcgt atataataat attattcctg
     3361 taccagtcaa aatcaattgt tcctttatag ccgtataatc ttgcgccacg cctgccaact
     3421 gcctggtttc tgacaaaaaa gttttgagaa taactcacct gaacaccatt atcaagttca
     3481 atcagagcat ttccgctgtc ttcatttttt atctcttttg aaaacataca tttttgccag
     3541 tcattccatt caaccttttc acccttgaat cgcttataaa aaagattaaa tggactttca
     3601 atacattcca ttttttcatc acaatccctg cataacagtt caaatggttt attccgtttt
     3661 ccataaatcc tctgagaatt cattgccgca atcatctttg gcttccttcc tgtcaggtaa
     3721 aaaatatagt caaaatcatg ggttgccttc tgcaggaaga gaccgccaac ttcatgatag
     3781 ttacgatacc agctattaaa ataaacagac ccataaggaa catcattcca tgcgacaaca
     3841 tgctcaactt ctcctattct tccagaatca ataatctgct tcactttctg cgccattgga
     3901 gtgagacgaa gcgggaatga aataactgtg ggcgctgtaa catttttgaa cgccttttca
     3961 agcattttga cctgatcaaa agtgatcgca acaggttttt caaggaaaag tggtaaattt
     4021 ttctttgcaa ccttgcatgc catttcagtg tggagataac atcttgttcc aatcatgaca
     4081 ccgtcaagtt caattttctc aagcatttca tcggcagaag taaaatatct ggtatctttc
     4141 atcaaatcag ggtcaatcct tctgtggctg tgaatttcag gaatattttc cttcactgat
     4201 ttttcaatct tttctgctgc aggatcagca atggctgcaa ttttataggg aacatcaaaa
     4261 atcttcattg ctgctgccat atgtgaaaca cgaagtccga aaccaatgat tccaaccttt
     4321 aacatacgtc ctccttttat tgtgatggta aaatactatc acttttttac tttgtgtcaa
     4381 cctgatattt gactttttac cattttacca ttatactgtt tctatggctg caatttattc
     4441 tattaccaat caaaaaggcg gtgttggaaa gaccacaaca tgcctttgcc ttggttctgc
     4501 tcttgctgaa aaaggatata aggtattgct gattgacttt gacccgcaat ccgggttaac
     4561 agtatgtctc gggtttgacc cagacgcttt ttctacaact atttacaatg tcattattaa
     4621 ctgcgaacaa taccccctgc aggaaatcat tacagataca aaggttccta atcttaaaat
     4681 tgctccagcc aatcttgacc ttgctggcgc agaaggagaa ttgatcggtg aaattggatg
     4741 ggacagaaat ctcagagatg ccataaatcc cattaaaaat gaattcgact tcatttttgt
     4801 tgattgtccg ccaagtttgg gtgttctgac aaccaatgca ttaatcagcg cccaaacagt
     4861 gattgtacct gttcaaacag aattccttgc tttaaggggc ttaaaacaac tgggagatat
     4921 cattacaaag gtgaggaaaa aggggaatcc ttctttaaaa aaaagaatcc tgcgaaccat
     4981 gcacgatgtg agaacccttc actcaaagga agttgttgaa gaacttgaaa gaattttccc
     5041 tgatgaaatt tttaacacaa ttatcaaacg cactgtcaag tttgcagatg catctcttgc
     5101 aggagttccc attttgcttt atgcaaaaag ttcagaagcc gcagaagcct acagacaact
     5161 tgcagaggag gtgattgagg atgccaaaaa gaccatctct taaaggcagg ggtgttgata
     5221 ttttccttgg gaaatcagac cagaaagaaa aaaaacctgt aaaagaagaa aaaaaaggca
     5281 aagccacttt ttacttcccg cagaaacttc tctcagaact tgatgaagca tggtttatcc
     5341 taagaagaat tgatagaaaa atcaaaaagt cagacattgt ccaggcagca ttggaaattt
     5401 ccattcaggg attgaaagag gggaaaaaag aaaatccatt gatagaatat ttcaaaaaag
     5461 aaaaatgaaa ccctgtcttt ctcttaccgc tttcgggaac ggttttcacc aatcaattgt
     5521 ctaatgaata taaaccggat gaatgagttt gatgatgaaa ctcttgtgcg aagggtgcag
     5581 gaggaaaacg atatgtatgc gtttgagatg attgtaacaa aataccagaa taaggtttat
     5641 ggaatctgct tcagatttat gggggaacat catgaggcgt ttgactgtgc tcaggatacc
     5701 tttgtaaaaa tttatcaaaa catccgttca ttcaaacacc tttcttcatt cggaacatgg
     5761 gtctatagaa ttgcagtgaa tacctgtaaa aatcgacttt cttcctggga ctttgtgagg
     5821 aaaaaaagaa ccacctatct tggaaatggc tgcactgaca ttgaaaacca gtgtgtttct
     5881 cctgcgcaac aatataaaaa aacaacaatc gttgaaattg ttcaaaacgc aatcaataaa
     5941 ctcccttttg accagaaaca aattgtgatt cttcgtgaca ttcagcaatt atcatatgaa
     6001 gaaattgaaa aaattacagg actaccactc ggaacagtaa aatcaaaatt atcaagggct
     6061 cgcaggaaac tgaaagaact gttaaaggat gtcgaacatg aactgtaaac aggttcaaca
     6121 gatactttct gattatcttg aaggatttgt ttcagatttg caaaaaaaag aaattgaaca
     6181 acatcttcag cagtgcgaac aatgccagat tgaactggat gaactgaaaa aaacagtgga
     6241 acttctgaag aatcttccag caccaccagt ttctgaaaaa ctggtagaaa gcgtaatgga
     6301 aaaaattagt acgacaggca tcagaaaaag aaagtttact cttttattga ctggcatcgc
     6361 gtgtgcagca attattgcat ttgttgtact tttcaaactt agccagcctg taaaaattgc
     6421 gtatcagcca gaaacaaaga tgaaaagtgc tcaaacaact gtggaagaaa aaaagacaga
     6481 aattacacca aagacaccag tttccagaga aaaaaagata gcagttatat ccagtaaaga
     6541 aacagaaaaa caggaaaaaa cagaaattgt gattcaactg gcatatcttg ggcaaccaca
     6601 gaaggtttat gaaaaaagaa attcgattaa ggaaaaacct actgacattg caatggatac
     6661 catggaaaca gcgcaacaaa aggaaatcat catgcgtgaa ccgcgtatag aaaaagcaga
     6721 atctgaagac aaaaaatcac aggaaattgc aaagccgctt gcagcaagac ccgttatcaa
     6781 gcctgaacca gttgaacaat tgagcactga atctgaaata ataaaacaca ttcatgctgc
     6841 tggtggaaaa attctttcag aatcacctgt ttctgataca gaaatcagcc atgtatttgt
     6901 tgcagaaatc cctggctcaa tgtataaaaa ttttcttgac catcttgcag gaacatttca
     6961 gataaaaaac tatgattcca tcaaggatgt cacaatttct gaaactttca tcacaataag
     7021 attacaactt ttcagataaa tgcctttatg ataagaaaaa ccccgaagag agaaagaaat
     7081 atcccgcaac agaccgtaac aattctataa acgggtggtg tgaattttgt ttttcctttg
     7141 ctgattgaat aagaaaccag cgtgtaccag aaaaaatcag cagaaatatg tccgagaaaa
     7201 aatgcagtca gtcccgcaaa ccccctgggc agagcaattg aaagatatgt aagcccgatt
     7261 gtcaaccacc atatggtcca gtatggattt gaaacactta cgaccatacc gttaataaca
     7321 gacaaggttt tagtggtatt aacatttttt tcaataggaa ggtctgtctt tgtttttttc
     7381 agcatcaaac ctgccatcaa cacgagaaca caaccacctg ccacacttaa aaatctggaa
     7441 accccaggtt gtttgagata tgttccaaaa ccagcgacaa gtaatacaac catcaaaaat
     7501 tccagaattg aatggcctgc tatcaaaaga ggaccctgcc tcgctccata cttaagagac
     7561 cttgtaatcg tcactgcaaa aaggggacca gggctcaaag cacctgaaag agccacaata
     7621 aaagaactca gaccaatcgc aaaaagattc atcacagaag ataacttttc aaaactttta
     7681 tgtcatgcgg aagcaccaca tctggttccc atgcattgat gtattcgcca gaaaagaatc
     7741 cctggtaatt gatttcctga agcaatctta atgcagtttt gtaaggaatc tctccttgtc
     7801 ccatcagggc aagagtaact tttctatcct ggtcatagat gccatcgtga atatgacagt
     7861 gctcaacaaa ttcttttaca atgtcaaatg cctcttttat tgtcataccc tgtgtatatg
     7921 gatgcatgat atcccagttg attttaacat ttggacttcc agcaactttc actgcctttg
     7981 ccacagtatc cgcccttgag aaaaaatcgt gtgtctcaag gcagataaat accctgtttt
     8041 tttctgcata ttctccagca attccaagac attccccaac gatttttatt gcctcgtcca
     8101 cgctgatttc tttgtcagga acccctccaa acaccctgat tctttttaca ccaatatcac
     8161 atgcaaggtc aataaaagcc cttgtcatcc caatagattc ttttcttttt tgagcgtctg
     8221 taaaacaata cctgatagaa gtggcgatgc acgaacattc aatgccactt tcatcaaaaa
     8281 atttttttat ttcttttctt ttttctttgc ttgcttcaat ttcaatacca tgattgtgct
     8341 gtgcctgagc gcgcggttca acaccatcat agccatattt tcttgcatag tccagcattt
     8401 tataaaggtc tgcctcaggg catgaaaaac tcataaaagc aaatttcatt tctgctcctt
     8461 tgtaaatttc actcctttaa ttttaatgaa ccaaatatat ctattttttc ggatgattct
     8521 tcaatgagcc ggtccacaac gacatcatta agtttatttt caggaagttt agcattttca
     8581 agatatttta gcatttgggt aaaaagatat ctcgcctcag gtaaattctg atcaaaattc
     8641 aaactggtga ttatgatatg tccttttccc caggaaaatt caataagata tgcaagagga
     8701 tacattctga aacatgtaga aaaggagcga atcactggtt cttctttcaa atctccgaaa
     8761 ggtctcaggt caattggcgg aaattcagca atcattctat aaaactgaag gtcacagaag
     8821 ttttcatgcg ggaagtcgcc aagcatcgga tgatttctaa caattgttcc agtattaccg
     8881 tcttcatagg ttgggtaatt ggccgggggc gtgaaaaaat atcgtccggc tacaagcccg
     8941 agtttcggga aaaatggcct tgtgaatccc tcaggtacag caagaattaa ttttccacca
     9001 tctgccacat aatttgcaag atttctgtca aagatttctg atacaacaag tttcgggaaa
     9061 actccgcgga tataagattt tttgatattc tgtgccttta atgtttttat ccaggttttc
     9121 tttgccttgc catacaccac aaattcaacc atattggtgg tttctggaaa tatccagtaa
     9181 tcccagtggt tttcaaaagt tctaccttcc tcttttgctt ttatacgcaa ccgtactttt
     9241 tcaggagatt gcacgtctgg taacctgatt ctaatattgc caattttatc tgtgcaaaat
     9301 ggtttgtgtg tgaaagaaaa ttcgccatgt tcctttactg tttcacctga aaaatcccat
     9361 tcaattgcag gattttttaa cggtggatgt gaaaaatctg aaataaaaat agttttttca
     9421 aaatccatac ccgaaacata aattctgtca tcaaaattac tatcagttaa aattgctgta
     9481 ggtcctgtgg tttgcctcca cacttcagct gtaacaaatt ttttttcgta aaattcatcg
     9541 ataactccct ggggagaaaa tccaatatcc attgctgtaa aatggcaaat tccggcaaga
     9601 ttaggataat cacggcgaat ctgttcgagt tttgtttttg cttcaataaa ctgaagtttc
     9661 tgactgtttg tgattaattc tggaagcagg tgatcaagtt gtgccttttt tgcattttca
     9721 agagcaattt caattgcata gggtcttact gcacctgtaa atttcccttt attgctgata
     9781 tcaggaaatg atgtccacca cctgaattcg tgttgaatca caggcataga aactctatta
     9841 tcttcttttg cctctgcaca ataaaaatca agtggcattc ccttaatttt tcttactgtt
     9901 ccatctgtcc atattacaaa acttgaaggt tttattgcct ttgtttccct gtaacatttc
     9961 cacgctgtct ctggatacaa aattactgga tgtccatatt cattgctcat gcaataaaga
    10021 tttgctgaaa catgattaac atccctgatg actgctgcgt tccactgata tcttaacaat
    10081 tcccttcttt ctgcatctgg ttgaggccat tgatattgat gatactggga atggccaccc
    10141 catgctccca tcattccgat ttcgctctgg accagtaatc ccacttcatc tgcaacatca
    10201 agatattcag gcactggcac gtaggactgg catcttatgt aaatatagcc atattgacgc
    10261 aaaaccgaaa gtttttttct ccatcgttgc ctgtctgtat cagggcaccc tgtttcagga
    10321 tttactgaaa aatcacctgt gcccatcata tagtatggct gattattgat cagaaaatgc
    10381 ttgtcctttg tcgaaagatg taagaatccc accctttcac atatacaatc aacaatttcc
    10441 tcgtttcttt tcagttcgac atccacctga taaagaaatg gggtctcagg tgtccataat
    10501 cggccttctt tcacaggaat actgaaaaca atttttccat gccctgatgt tcttgtaatt
    10561 tgaccacatt gtttacctga agggtctttt acaaaaacct ctaaatcaac atcttcttga
    10621 ttaccccctg tttttatcat gaaatccata catttaccaa caggatcaga atgtatccag
    10681 cagtgttcga cccatgcat
//
LOCUS       DAORYU010000023        10461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_4801_length_10461_cov_4.793677, whole genome shotgun sequence.
ACCESSION   DAORYU010000023 DAORYU010000000
VERSION     DAORYU010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10461
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4801_length_10461_cov_4.793677"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="PLW07_01415"
     CDS             complement(<1..453)
                     /locus_tag="PLW07_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49215.1"
                     /translation="MTRKQLNLAIFEGKAESIFWQPRLETWILFHRAKGTLPDRFRDL
                     TDLEIYDRLKCSVRYAASSGLHYYYTTTVETFERPVDRNHTEFITKTEKGILKTVYQI
                     VPGGMGYSDNNRIVEFPVKTVEDLKILTHLENHFNAVAIPELFEEAANR"
     gene            complement(782..1804)
                     /locus_tag="PLW07_01420"
     CDS             complement(782..1804)
                     /locus_tag="PLW07_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020561915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HOL49216.1"
                     /translation="MKAMVIEKIVDVLQEDPLKVVEMPEPTLQEGQLLIKINACGVCR
                     TDLDEIEGRTAPSFYPVIPGHQVIGTIVEVKNPSKFKTGDRVGVGWIFKSCGKCSYCI
                     EGHENLCDVFQATGRDAHGGYAEYMAAYEDFIYKIPDIFSDIEAAPLLCAGAIGWRSL
                     RLTGAKQSGAIGLFGFGASGHIVIQIAKCLYRETKIFVFTRSEKEQALALQLGANWAG
                     QITEQPPELLDVAIDTTPAWLPVISALKNLKKGGRLVINAIRKENKDIDIMKEIKYET
                     HLWMEKEIKSVANVGRRDIAEFLEIAANIPIKPQITTYSLDEANQALKDLKTGTKAGA
                     KVLVMD"
     gene            complement(1804..2583)
                     /locus_tag="PLW07_01425"
     CDS             complement(1804..2583)
                     /locus_tag="PLW07_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase cyclase
                     subunit"
                     /protein_id="HOL49217.1"
                     /translation="MQKPVRIMPCLDMQSGRVVKGVHFVDIKDVADPVECAVAYCKQG
                     ADEIALLDITATVEGRPTLIDVVRRVAEVVTIPFTVGGGISDMNIAESVIKAGADKIS
                     VASAGLRNPDLIKQLVKEFGSERITVAIDVDHNPAMPSGYEIYIDGGRTPTGKDAVEW
                     AKQVDDYGVSVILPTSKRGDGAQSGYDLPIIRALKKEVSAEIVASGGAGNLEDFYHAV
                     EAGATILLAASVFHFRIISIPALKAYLKSKGVLIVDRQLEK"
     gene            2831..2962
                     /locus_tag="PLW07_01430"
     CDS             2831..2962
                     /locus_tag="PLW07_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49218.1"
                     /translation="MSLRDVSGIIKNIVPETKGDMNNDGVVDITDVIKVLRKAINLE"
     gene            complement(3313..4611)
                     /locus_tag="PLW07_01435"
     CDS             complement(3313..4611)
                     /locus_tag="PLW07_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012900112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HOL49219.1"
                     /translation="MIRKIERYFKFSERNTNWITELRAGFATYLTMAYILFVNPVILK
                     DAGVPFNACVVGTALAAGLTSIIMGIVANFPLALAAGMGLNSVLTYSIVIGQGVSWQV
                     GMGLIFINGLIVFILVLVGLREAVMNAIPVPLRHAIAVGIGMFLAYIGLMHAGIVIID
                     RNTLLPSPGNFSEISTIISCCGILITAVFVALRIKGAIIGGILITAFLSFLAGLSKLP
                     ATINTLDFSTVGALDVVGALKLSLIPSLFAFIIVDFFDTLGTVTAIGYQAKLVDSQGS
                     IEKLKAILGIDAIGAMLGGFFGSSSNTSYIESAAGIMEGGRTGITAIVVGVLFLLSIV
                     IAPVASMITSQATAPALVIIGFLMMQAITQIDFEQLDTAIPAFVIILAMPFTYSISHG
                     IGYGFIVYTVLKMAKGQFKDVHPLMYIISLIFAVYFLAGS"
     gene            complement(4621..5616)
                     /locus_tag="PLW07_01440"
     CDS             complement(4621..5616)
                     /locus_tag="PLW07_01440"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOL49220.1"
                     /translation="MAEKSFSFKKIDIPAERHLIDKLLEFWVEIFGDEHVVNQGLLSI
                     LTGKDRNFNRDFLFIAQHGTIIVSTVHLTISKFDKRIGGIGEVATLKQYRGKGIARIL
                     CSMAIKTFEDSGGKWLFLGTSNPAAARLYYSFGWHYLTGTKIMLRNSSGNTPEVFFNK
                     YLSSIKTGKISILRGDSRFRLQVIPLVAIPYDEPVLDLNAGLFSTRWYTQRSCMGLYS
                     HYEKLEENGAWFVAVCRKFLVGIVSIVFHDNNIAQIDGFCIPGTGKKVLLNFYKTAID
                     YAKNNNASEIHMVADDLDENKKQMLLKIGCIPTDERVKIESKEGILDISVYRYRQ"
     gene            5847..6242
                     /locus_tag="PLW07_01445"
     CDS             5847..6242
                     /locus_tag="PLW07_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOL49221.1"
                     /translation="MENETNQNPDEAKTIMIVEDDDTIRKILVIECKRKGFDVLEYRD
                     AASAMTGIDRNKPRIDAAVVDLMNLGYGGNLGEYLRKYQEYTETKVIYYSALTEKQFN
                     RNILDVPKTYYVHKIPGSIKKVMEILETI"
     gene            6263..7315
                     /locus_tag="PLW07_01450"
     CDS             6263..7315
                     /locus_tag="PLW07_01450"
                     /inference="COORDINATES: protein motif:HMM:NF024126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HOL49222.1"
                     /translation="MPNYNDAPQVIDNFIYKLYKRTKPSMAVETIKDGKFFQQWRDTL
                     KAKIRELLGEFPEPAPLKPQLCSTEELDTFIREKYLIQTEQDCCMPLYLLTPKTDEKK
                     LPAILCCHGHGPYGKDPVAGVHFRQPERLSNINTHNYNYGEIMARRGYITIVPDWRGF
                     GERCGYYDNVFPGRDKCNVYFIQHLILGRTLLGGNIFDGMRAIDFLLTRKNVDPDRIG
                     CMGLSQGGTMTTYITLLDDRIKAADIICYATTTEHYAINRPNFCGSQLVPGLYRYADV
                     GDVIGAIAPRPLLIESGASDTCFWLHSSLQAHEKAKRAYKLAGKEENLWIEVFPGEHS
                     FAANKAFEFFDTYLKR"
     gene            7312..8970
                     /locus_tag="PLW07_01455"
     CDS             7312..8970
                     /locus_tag="PLW07_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49223.1"
                     /translation="MKKRKKVVILCLCTLIFLWIFIPVITGIWANTASDGKVKLFFFL
                     TPPGAKLVNFHWKILEKPLLSLKLSGSLKVPWKSIIFGSQQLLAFQAPANINLPQGKS
                     RVNFSGEIQGNFKTGQINIKNGNIWLEKFGRIFVSGKLVQWGKEICEIEGNVKDFAID
                     EFRTMLGIQNLPFAGTMTGKLSVVINKDTVKLLKFDIDFNKLLLQEYSHPLSGHAKGT
                     YDFLQKKCLIETGNIFTESGGKLFLKGIISPEDFSVKIESEGVNLEEIISQLPEKWRE
                     KFKVKPDAKISINGEGEWKRTEKLPLFSGVFAIPDKIQYKNFSCSSLLIEGSQETEQI
                     TVSASNIDIGKINCDSISGIITKENGKYRGDFTFTFYGGSGNMVLVTTQDTPIRVYAK
                     AHVQKINLEKLIHSLNPDILITGAVNVICFLELGVKNFSMQAKIDNVPGMPFSQKLNI
                     GAVKALASLGSSSFAGSIGRQFGSNNFYYRKLSGVISLTNGQLTIEGTAKRAGNNDYL
                     ITSEIFGSGINVLVDRKNNSIHVEDLKRRILNAMQQNKAQFKLS"
     gene            8985..10388
                     /locus_tag="PLW07_01460"
     CDS             8985..10388
                     /locus_tag="PLW07_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HOL49224.1"
                     /translation="MEKQTIGLIVGNRSCFPEILCKAGRERMIKILEKTGVEVIALSE
                     NDTRYGKVETWQDAKKCAALFKKNADKISGIIVTLPNFGDEQAVADTIRLSNLDVPVL
                     IHAYPDEPEKMDPANRRDSFCGKISVCGNLKQYGIRYSLTRNHTVSPEEEDFQYDLEE
                     FLATCRIVRGLKNLKIGAIGTRPDPFKTVRYSEKILENNGVSVDVMDLSELFALTNKL
                     NDSDARVKRTFEEFANYHKVQKEKEAVMKMAKLIVSISDWIETKELKGFAFQCWTSIQ
                     ENYGIFPCGVMSYFSHKMIPAACEVDVTGLLSMYILQLASGKPSAIVDWNNNYGNDPD
                     KAVLFHCSNFPVEILDTPIMTHHVILEQTVGKEKTFGAVEGKIKPGPLSLLRLTTDDC
                     DGEITGYVCEGTITKESLKTFGGVGVARIDNLQELLMFICKNGFEHHVAINLSHTAGP
                     IFEALAIYKGWDIYPHI"
BASE COUNT         3425 a         2046 c         1952 g         3038 t
ORIGIN      
        1 cctgtttgct gcttcttcaa aaagttcagg tattgccaca gcattaaaat gattctcaag
       61 atgcgtaaga attttcagat cttcaactgt ttttactgga aattcaacaa ttctattatt
      121 gtcagaatac cccattccac caggaacaat ctgataaacg gttttcaata tccccttttc
      181 tgtcttcgta ataaattctg tatggtttct atcaaccggt ctttcaaacg tttcaacagt
      241 ggtcgtataa taataatgga gccctgaaga agctgcatat ctgacagaac atttcaatcg
      301 gtcatatatt tcaaggtcgg ttaagtcacg aaatctgtcg ggtaatgttc ctttcgctct
      361 gtgaaacaaa atccaggtct caagacgtgg ttgccagaaa attgattctg cttttccttc
      421 aaaaattgca aggttcaatt gctttctggt catactcact cctttctgac aatttgactg
      481 gtttatcttt gaaacttaat ataggatata acaggaggaa aatcaaatat attagcgtat
      541 taattgttgt cctatggttc acttgttcag catagcaaaa agacaactac cagtacagcc
      601 acaaatcttc ccataaaaaa tcctccgggt attatcgtca ctgctggtgc cgaagaatgc
      661 gctggagaaa caaaagaaaa ttttccaaaa aacatcaatc agtggcttgt aaaacacaat
      721 tcggaccagt tttatgaaaa actgtgggta agatgtaaat tgagttttta tcacaaaaat
      781 tttaatccat tacaagaact tttgcccctg cttttgttcc tgtcttcaaa tccttcaatg
      841 cctggtttgc ttcatcaagg gaatatgttg taatctgtgg tttgatcgga atatttgcag
      901 caatctcaag aaattctgcg atatccctgc gtcccacatt ggcaactgac ttaatttctt
      961 tttccatcca cagatgtgtt tcatatttaa tttctttcat gatgtctata tctttgtttt
     1021 cttttctgat agcattgata acaagcctgc cacctttttt aagatttttc aatgctgaaa
     1081 tcacaggaag ccatgcaggg gttgtatcaa tggcaacatc gagcaattct ggtggctgct
     1141 cggtaatctg gcctgcccag tttgcaccaa gttgaagcgc aagtgcctgt tctttttcag
     1201 aacgggtaaa tacaaatatc tttgtttctc tgtacaggca ttttgcaatt tgaatgacaa
     1261 tgtgtcctga agcaccaaac ccaaaaagtc cgatggcacc agattgtttt gcgcctgtta
     1321 accgcaaaga acgccagcca attgctcctg cgcacagtag tggcgctgct tcaatatcag
     1381 aaaaaatgtc aggaatttta taaataaaat cttcgtaggc agccatatac tcagcgtaac
     1441 caccatgcgc atccctgcct gtggcttgaa aaacatcaca gagattttcg tgcccttcaa
     1501 tacaataact acacttacca catgatttaa aaatccagcc aactccaacc ctgtctccgg
     1561 ttttgaactt tgatgggttt tttacttcaa caattgttcc aataacctga tgcccaggaa
     1621 taacaggata aaatgagggt gcagtcctac cctcaatttc atcaaggtct gttctgcaaa
     1681 caccgcaggc atttattttt ataagcagct gcccttcttg taatgttggt tctggcattt
     1741 caacaacttt caatggatct tcctgcaaaa catcaacaat tttttcaatc accattgcct
     1801 tcatcatttc tccagttgtc tgtcaacaat aagtactccc tttgatttca ggtatgcttt
     1861 aagagcaggt atggaaataa ttctaaagtg aaaaacactt gctgcaagaa ggatggttgc
     1921 acctgcttcc acagcatgat aaaaatcctc aagattgcct gcaccacctg aagcaacaat
     1981 ttctgcagaa acttcttttt tcaaagccct gatgataggc aggtcataac cagattgcgc
     2041 tccatcgcct cttttgcttg ttggaagtat tactgataca ccgtaatcat caacctgctt
     2101 tgcccactca acagcatctt tccctgtggg tgttcttccg ccatctatgt aaatttcata
     2161 gccagatggc atggcaggat tatggtcaac atcaatggca actgttattc tttcagaacc
     2221 aaattctttc accagttgtt tgatcaaatc aggattcctt aatcctgctg aagcaactga
     2281 aattttatca gcacccgcct taatcactga ttcagcaatg ttcatatcag atattccacc
     2341 acccacagta aatggaattg tcacaacctc tgccaccctg cgtacaacat caatcagggt
     2401 tggtcttcct tctactgttg cagtaatatc aagaagggct atctcatcag caccctgttt
     2461 acaatatgca accgcacatt ccacagggtc agcaacatct ttgatgtcaa cgaaatgaac
     2521 acctttgact acccttccac tttgcatgtc aagacatggc ataattctga ctggtttttg
     2581 catttttcct cctgttaatt ttaattctac tctatgatga aattaaatac aactttaact
     2641 tgatacttat atttcaagtc aaaatatatt gtaaattaag agaactatgt ataaaaagat
     2701 taaaaagaga tgataaaaaa attaacattt tttcatagtt ggagttttta ttttattttt
     2761 ccgttatttt ttatcaaaga tacatagaga aataagattt ttttaatatt agcaatccga
     2821 atattgaatt ttgtccttgc gagatgtttc tggcatcatt aaaaacattg tgcctgaaac
     2881 aaaaggcgat atgaataatg atggtgtagt tgatatcact gatgtcataa aagtcctgag
     2941 aaaagccatt aatcttgagt aaatttgttt gcaaatttta tcagggtttt ttgcagttta
     3001 atgatttgca ctggaggtga tacttttcag atttttcaca atgacaaacc ccagaacaat
     3061 gattacaatt aacatactga cagttaaaaa attttctgtt tttctcgaat ttctcaaaaa
     3121 aagaaaccct gtagtggggg ttattttctt gatttccagc gtacaccaca gtgattattc
     3181 ccttcaagta tggctgatat tttgcaaaat catacacagg tcagaaaggt ctggttttga
     3241 atttataagt acttctgtgt caagtttcac ttcataaata tctgcttttt catcaccctg
     3301 ataaattttt catcaactac cagcaagaaa atatactgca aaaatcaggg aaataatgta
     3361 catcaaagga tgaacatcct taaattgtcc ctttgccatt ttcaaaacag tataaacaat
     3421 aaatccatat cctataccgt gggaaataga atatgtaaaa ggcattgcaa gaatgatgac
     3481 gaaggcagga atcgctgtgt ccagttgttc aaaatcaatc tgtgtaattg cctgcatcat
     3541 taaaaatccg ataatgacaa gcgcaggagc agtcgcctgg gatgtaatca tggaagcaac
     3601 tggagcaata acaatactca aaagaaataa aactccaaca acaattgcag taatacctgt
     3661 tctaccacct tccatgatac ctgctgctga ctcaatgtag gaagtgtttg aacttgagcc
     3721 aaaaaaaccg ccaagcattg cgccaatggc atcaatgccg agaatcgcct tcagtttctc
     3781 aatactgccc tgtgaatcaa caagttttgc ctgatatcca atggcagtca cagtaccaag
     3841 ggtatcaaaa aaatccacaa taataaaagc aaagagcgaa gggataagcg aaagtttcaa
     3901 agcaccaaca acatcaagtg ctcctacagt tgaaaaatcc agcgtattga tagtggcagg
     3961 taatttagaa agaccggcaa ggaatgaaag aaacgcagta atcagaatgc caccaatgat
     4021 tgcaccctta attcttaatg ccacaaacac agcagtgata agaattccgc agcacgaaat
     4081 aatggttgaa atttctgaaa aattacctgg cgatggtaaa agggtgttcc tatctattat
     4141 tacaatgcca gcatgcatca aaccaatgta agcaagaaac attccgatgc cgactgctat
     4201 ggcatgtctc aatggaactg gaatcgcatt catcactgct tcccgtagtc ccaccaacac
     4261 caggatgaat acaatcaaac cattgatgaa aatcaaaccc attccgacct gccatgaaac
     4321 accctgtcct ataacaatac tgtatgtgag gacactatta agtcccattc ctgcagcaag
     4381 agccagcgga aaattggcaa caattcccat aataattgaa gtcaatccag cagcaagtgc
     4441 tgtgccaaca acacaggcat taaacggcac gcctgcatcc ttcaaaatta caggattaac
     4501 aaagagaata tatgccattg ttagatatgt ggcaaaacca gcgcgtagtt cagtgatcca
     4561 gtttgtattt ctttcagaga atttaaagta tcgctcaatt tttcttatca taacgctcct
     4621 ttattggcga tatctgtaaa cacttatatc aaggattcct tcttttgatt caatctttac
     4681 cctttcgtct gtcggtatgc aacctatttt caacaacatc tgtttcttat tttcgtcaag
     4741 gtcatctgca accatatgaa tttctgaagc gttattgttc tttgcataat caatcgctgt
     4801 tttatagaaa ttcagtaaaa cctttttgcc agttcctgga atgcaaaatc catcaatctg
     4861 ggcaatgttg ttatcgtgaa acacaatact cacaatgccc acgagaaatt tcctgcatac
     4921 tgcaacaaac caggctccat tttcttctaa tttttcataa tgcgagtaaa gacccatgca
     4981 ggaacgttgt gtgtaccatc gagtagaaaa caatcctgca tttaggtcaa gcaccggttc
     5041 gtcataggga atggccacaa gaggaataac ctgaagccta aaacgagaat caccgcgcag
     5101 gatactaatc ttaccggttt ttattgaaga aagatactta ttaaaaaaaa cttctggggt
     5161 atttccgctg gaatttctga gcataatttt tgttcccgtc aggtaatgcc agccaaacga
     5221 ataatacagt cttgctgccg ctggatttga agttccaaga aacaaccatt ttccgccact
     5281 atcttcaaat gttttgattg ccatggaaca aagtattctt gcaattcctt tacccctgta
     5341 ttgttttaat gttgcaacct cacctatacc accaatacgt ttatcaaatt tgctgattgt
     5401 cagatgaact gttgaaacga ttatggttcc gtgttgcgcg ataaacaaaa aatctctgtt
     5461 aaaatttctg tctttgccag taagaatgga taacaaaccc tgattcacaa catgctcatc
     5521 gccaaaaatc tcaacccaga attccagcag tttatcaatc aaatgtcttt ctgctggaat
     5581 atctattttc ttaaatgaaa aacttttttc agccatgtcc tgaaatcatt ttacatcttt
     5641 ttcttttttt aaggaaactg aataaactat tttgcaaact tattcgtcca ttatataact
     5701 gaatgaaatg gttcctccat ttaaaggacc cggcaacagc atttatcctc aagatgcata
     5761 gccgggtccc tgtgtttttt gacatatttg taacacagga cttataatac aaagcaggga
     5821 taattttatg gtcaaaaagg aaaaacatgg aaaatgaaac aaaccaaaat cctgatgaag
     5881 caaaaacgat tatgattgtt gaagatgatg atacgataag aaaaatcctc gtgatagagt
     5941 gtaaaagaaa agggtttgac gtgcttgaat acagggatgc agcatcagca atgactggta
     6001 ttgacagaaa taaaccacgg attgacgcag cggttgttga cctgatgaat ctcggttatg
     6061 gcggtaacct tggcgagtat ctgaggaagt atcaggaata cactgaaaca aaagtcattt
     6121 attattctgc tcttactgaa aaacaattca atcgaaatat tcttgatgtg cccaagactt
     6181 attatgttca caaaatacca ggtagcataa aaaaagtaat ggaaattctt gaaacgattt
     6241 gaaaacaaaa aaggagtata ccatgccaaa ctataatgac gcaccgcagg tgattgataa
     6301 ttttatttat aagctttata aacgaaccaa accttcgatg gctgtggaaa caatcaaaga
     6361 tggaaaattt ttccagcagt ggagagatac attaaaagca aaaattagag agttactggg
     6421 agaatttcct gaacctgctc cgctcaaacc gcaattatgt agcacagaag aacttgatac
     6481 ttttatacgg gagaaatatc tcatacagac cgagcaggac tgctgcatgc cactctatct
     6541 tctgacacca aaaacagatg aaaaaaaatt accagcaatt ctttgctgtc acggacatgg
     6601 accttatggt aaagaccctg ttgctggagt tcatttcaga caaccagaac gactaagcaa
     6661 tatcaacacc cataattata attatggtga aataatggca cgacgcggct atatcaccat
     6721 agttcctgac tggcggggct tcggtgaacg atgtggatat tatgataatg tatttccagg
     6781 cagggacaaa tgcaatgtat atttcattca acacctgata cttggcagaa cactccttgg
     6841 tggtaatatt tttgacggaa tgcgagcaat cgattttctt ttgacaagga aaaatgttga
     6901 ccctgataga atcgggtgta tgggactttc tcagggtgga acaatgacaa catacatcac
     6961 gcttcttgac gatagaataa aagccgcaga tatcatctgt tatgccacaa ccactgaaca
     7021 ttacgcaatc aacaggccta atttctgcgg aagtcagctt gtccctggac tttacagata
     7081 tgctgatgtc ggtgatgtta tcggtgccat tgcaccacga cctttgttga ttgaatcagg
     7141 agccagtgat acctgtttct ggcttcactc ttctctacag gcacatgaaa aagcaaaaag
     7201 agcatataaa cttgcaggaa aggaagaaaa tttgtggatt gaagtatttc caggagaaca
     7261 cagttttgct gcaaacaaag catttgagtt ttttgacact tacttgaaga gatgaaaaaa
     7321 agaaaaaagg ttgtgatatt atgcctctgt acgctcattt ttctatggat ttttatccct
     7381 gttattactg gcatctgggc aaacacagcg tctgatggaa aggtaaaact tttcttcttt
     7441 ctcaccccgc ctggagcaaa acttgtcaat tttcactgga aaatccttga aaaacccctg
     7501 ttatctttga aactatccgg ttcattgaag gtgccatgga aaagcataat atttggatca
     7561 cagcaattac ttgcatttca ggcacctgca aacatcaatc ttccgcaggg taagtcgagg
     7621 gtgaattttt caggtgaaat acagggaaat ttcaaaactg gccagatcaa catcaaaaat
     7681 gggaatatat ggcttgagaa attcggaagg atttttgttt ctggaaaact ggtgcaatgg
     7741 ggcaaggaaa tatgcgagat agaaggtaat gtaaaagatt ttgctataga cgagttcagg
     7801 acaatgcttg gtattcaaaa ccttcctttt gcaggaacaa tgacaggaaa actctcagtc
     7861 gtgataaata aagacacagt gaaattgtta aaatttgata ttgatttcaa caaattatta
     7921 ctacaggaat acagtcatcc cctgtcaggt catgcaaagg gcacgtacga tttcctgcaa
     7981 aaaaagtgcc ttatagaaac tggaaatatt tttactgaat caggaggaaa actttttttg
     8041 aaaggcatta tcagtcctga agatttcagc gttaagattg aatctgaggg ggtaaatctt
     8101 gaagaaatta tttcgcaatt acctgagaaa tggagagaaa aattcaaggt caaacccgat
     8161 gcaaagattt ctatcaatgg agagggtgaa tggaaaagga ctgagaaatt accactgttt
     8221 tcaggagtgt ttgcaatccc ggacaaaata cagtacaaaa atttttcctg ttcatccctt
     8281 ctgatagaag gttctcagga aacagaacag attactgttt ctgcctcaaa cattgacatt
     8341 ggtaaaatca actgcgatag tataagtgga ataatcacaa aggaaaatgg aaaatacagg
     8401 ggtgatttca cattcacatt ttatgggggt tcaggaaata tggtattggt cactacccag
     8461 gataccccga tcagggttta tgcgaaagca catgttcaaa aaatcaatct tgaaaaactc
     8521 attcacagtt tgaaccctga cattcttatc acaggggctg tcaatgtaat atgcttcctt
     8581 gaacttggag tcaaaaattt ttccatgcag gcaaaaattg acaatgtccc tggaatgcct
     8641 ttttcacaaa aacttaatat cggcgcggta aaagcacttg caagtctggg ttcatcaagt
     8701 tttgccggta gcattggtag acaatttggt agcaacaatt tttattacag gaaattgagc
     8761 ggtgttatca gtttaaccaa tggtcagtta acaatagaag gaacagcaaa acgcgcaggc
     8821 aacaacgatt accttatcac atctgaaatt ttcggttctg gcatcaatgt ccttgtagac
     8881 agaaaaaata attcaataca tgttgaagat ttgaaacgaa gaattctcaa tgccatgcaa
     8941 cagaataaag cacaattcaa actttcataa aaaggggggg tactgtggaa aaacaaacta
     9001 ttggattgat tgttggaaat agatcatgtt ttcctgaaat actgtgtaag gcaggaagag
     9061 aaagaatgat aaaaatcctt gaaaaaacag gcgtggaagt cattgctctt tctgaaaatg
     9121 acacaagata tggaaaggtt gaaacatggc aggacgcaaa aaaatgcgct gctcttttca
     9181 aaaaaaacgc agacaaaata agcggtatca ttgttacact tccaaatttt ggagatgaac
     9241 aggcagtcgc agacacaata cggctttcaa accttgatgt acctgttctg attcacgcct
     9301 atccagatga accagaaaaa atggaccctg caaacagaag ggatagtttc tgcggtaaaa
     9361 tttcggtttg cggcaatttg aaacagtatg gtatcaggta ctctctcacg agaaatcaca
     9421 cagtttctcc agaagaagaa gattttcaat atgaccttga agaatttctt gctacctgcc
     9481 ggattgtcag aggtctgaag aatttaaaaa ttggagcaat aggcacaaga cctgacccgt
     9541 tcaagacagt cagatacagt gaaaaaattc ttgaaaataa tggcgtatct gtggatgtta
     9601 tggatctttc agaacttttt gctctaacaa ataaacttaa cgatagtgat gcaagagtta
     9661 aaagaacttt tgaagaattt gccaactatc ataaagtgca aaaggaaaaa gaagccgtaa
     9721 tgaaaatggc aaaactgata gtatccattt ctgactggat agaaacaaaa gaactgaaag
     9781 ggtttgcctt ccagtgctgg acatccatac aggaaaatta tggtatattt ccgtgtgggg
     9841 tcatgagtta tttcagtcat aaaatgatac ctgctgcgtg tgaggttgat gtgacgggac
     9901 tactatcaat gtacattctg caacttgcat caggaaaacc cagcgcaata gttgactgga
     9961 acaataacta tggtaatgac cctgataagg cagtactgtt ccattgcagc aatttcccag
    10021 tagaaattct tgatacaccc attatgacgc atcatgtcat tctcgaacag acagttggaa
    10081 aggaaaaaac atttggagca gttgaaggaa aaataaaacc aggaccttta tctttgttaa
    10141 gattaacaac agatgactgc gatggagaaa taacaggtta tgtttgtgaa ggaacaataa
    10201 caaaggagtc gctgaaaacc tttggtggtg tcggagtcgc aagaattgat aatctgcagg
    10261 aacttttgat gttcatctgt aaaaacggct ttgaacatca tgtggcaata aacctttcgc
    10321 acacagccgg accgatattt gaggcacttg caatatacaa aggatgggac atttatcctc
    10381 acatatgaca ggtgttttga tgcatattga aaattgattg aaacgcaagt atcgcaagtt
    10441 gatggctggt ttttgatgct g
//
LOCUS       DAORYU010000024         9931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_5111_length_9931_cov_5.158465, whole genome shotgun sequence.
ACCESSION   DAORYU010000024 DAORYU010000000
VERSION     DAORYU010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9931
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5111_length_9931_cov_5.158465"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="PLW07_01465"
     CDS             <1..458
                     /locus_tag="PLW07_01465"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOL49225.1"
                     /translation="KNFGFADDLIATLSVEKYKYFVFPFHKRIVEEFSDGSPIYMHLC
                     GNAWHLFKFLKENLNVYSFDTGFPIDFTEVRKELGEDVEIIGGPSIMLLQSGKEEDII
                     KEVKKICESGIMKGKRFIMREGNNLAPLTPVENIKSMYMAARIYGQYSD"
     gene            596..670
                     /locus_tag="PLW07_01470"
     tRNA            596..670
                     /locus_tag="PLW07_01470"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:631..633,aa:Arg,seq:cct)
     gene            708..1043
                     /locus_tag="PLW07_01475"
     CDS             708..1043
                     /locus_tag="PLW07_01475"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOL49226.1"
                     /translation="MKKTSESNEKFRKGDSGPKYLFRGPHCEWGIIVLKPGEKMAAHG
                     HNAVVEDFFFLEGSPHIIVSSQQQRVFPGDAVRVEPGEVHDIINDTDQEVKLVFIKAP
                     YLPDDKFSC"
     gene            1063..3339
                     /locus_tag="PLW07_01480"
     CDS             1063..3339
                     /locus_tag="PLW07_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49227.1"
                     /translation="MEKDIFLKAVNPLKQDVTVALTASIPCSEGLLKSADSLTLYDEN
                     TVIPCQKRVLARWNDRSIKVVFIDFVATLHTGTNIYRIDFKGDRKTEYITQQSVKIRP
                     CEEGENQVIVDDRFYLKLVLVDSRNQKYRAKIKKVKKQNGRLTKVTTIYGSFVTKTNV
                     RGIDFTLTLTGWAGCRLIKIEPEILVNPTEGVFFRVRELKLSLTPISGGITDVFFPDV
                     QTNQTQKVKMFQIDDEHCTIQLDKNTETRNIVSTGTFMLNTDDQVIAGALKDFAVRWP
                     KSMEYDGYSINIGLFPSFKRGTFNHMQPWYKYGYLFHGSTYLLKTGQKRRWEIWLDMN
                     GNWQLCKSICEMPAVAVVDPEYAISTGVYGNICAAGKNTVDYDRVVSNMFDLYFSDIR
                     KNRDTGEMNWGDWFGERFVNWGNNEYDTAEQLFIQFIRTGDLRYFVSGENAARHTDEV
                     DIIHSVNRDLIESFNEEVFKPFDVMPGCVHEHTIGHVGGFCSRKKVKKMLSKVLGVKN
                     PYLCLDPWNMGHLWLEGLRDAYFFTGDEKFKQTVILVADYLSKVVAERLYPFSGHAHS
                     GRTVGWPLTALCAAYDIKKDRKYLKAMKILVNDMLAEQDPHCGGWLYELPDGHCNCVR
                     KKHVGSAGFLISILVNGLSRYYEMTGDKRIPQAVKKAMEFLVVDTWDERTGKWRYTSC
                     PVSPFVEPYGLILLSLANGINIGNVDVRDVFVKNWQHFVEGCDKDINQKMIGRLFSIR
                     LFGTAKALKIYHQIKSNE"
     gene            3332..4099
                     /locus_tag="PLW07_01485"
     CDS             3332..4099
                     /locus_tag="PLW07_01485"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HOL49228.1"
                     /translation="MNRKWLYVITQDVSPQMNMALDSIMWNKVKQYPDTAILRFYTWK
                     PSGVSLGAHQKPENLVNVSFCKQNNIPVVARITGGSAIFHDREITYSFSATNDEKVFS
                     GPITSYEKICGALKTGLEKLGIRVQWRGVSKGKEPSFTDRDCFSLSTRHDLVVDDRKI
                     IGSAQRKDRTSFLQHGSLLIEIQKHLWEKIFLAKPDFSKIISMSEILSNVPDFDTLIK
                     VLKNGFEQFFESDFEEIHFTSEDIKEAEKIQQTFLLV"
     gene            4096..5118
                     /locus_tag="PLW07_01490"
     CDS             4096..5118
                     /locus_tag="PLW07_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49229.1"
                     /translation="MKDEKLFNNPDFFRCYMCLFAAETRMEMSQYRWVKITDHAAFAP
                     RDGAGALVFKRKMWLLGGWNPSDKIHFPKICNSEVWSSKDGINWKLENPCAPWEGRHT
                     AGYVVFNDAMWIVGGDANQHHYQNDVWKSTDGVKWECVCENVPWGLRVLHYTVAFRNK
                     IWVIGGQTLPHFAPADEIFYSDVWCSDDGRHWEKVADNLPWAPRGMIGGSAVFKDKIW
                     ILGGGTYDTPKKPERIFYHDVWCSQDGKNWELVCDSAPWHPRQYHDVAVFDNKLWVME
                     GYHKNGGNRKDVWYSEDGKNWTEVSDTPWQPRHAASVFVYDNGLWMVAGNNMTPDVWK
                     LEKIIK"
     gene            complement(5127..5700)
                     /locus_tag="PLW07_01495"
                     /pseudo
     CDS             complement(5127..5700)
                     /locus_tag="PLW07_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013890337.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
     gene            complement(5705..6549)
                     /locus_tag="PLW07_01500"
                     /pseudo
     CDS             complement(5705..6549)
                     /locus_tag="PLW07_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195064.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            6704..7789
                     /locus_tag="PLW07_01505"
     CDS             6704..7789
                     /locus_tag="PLW07_01505"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOL49230.1"
                     /translation="MTAKERWIASLKLEPVDKLAFWPKISGSYLNYQGGSFKSMTVEQ
                     MHDYIGSDKHQWLVGFIKEKRRTTSFEEIKQGMTRKLVYSTKYGNLTATMRFDEPSQA
                     WHPVEYPVKARQDIEIMTEWFADACFEIDEEKLQEAKQMYKETGSDAITAHSIGTTPL
                     MQWVQHLAGIENCHYFLSDFTEEVERLFETMQKKNRKSAEILAQDSPAEILYLVENTS
                     TTLISPQQYRKYCYKDILDCAGIVNDSGKFLVLHMCGHLKNLLVDLARLPVSGFEAFT
                     TMPVGNTTLVEARKICPDKCLIGGTNAVLWTKSAEEIINEIEEELYKLPHHRGLVITS
                     AGVMPPVCKPETIRQVCQWVQQYRIRV"
     gene            complement(7805..8989)
                     /locus_tag="PLW07_01510"
     CDS             complement(7805..8989)
                     /locus_tag="PLW07_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HOL49231.1"
                     /translation="MEQFKITEYGAVPNTSTVSTKSIQKAIDSCFKKNGGRVICDSGV
                     FITGTLILKSNVELFLEAGSVLKGSENLSDYQNLEEPFIGKNPPEKSNNALICASYAE
                     NISIRGAGTINGAGPAFYDHKKVDANGKFEKPETQRPRIIMFYRCKNVLIEDIGFVDS
                     ACWTIWLMQCENVCIHRIKVSGDRRLRNIDGIDIDACKNVTVSDCIFDTEDDCIVVRN
                     MKHFYKEYCPCENITVTNCVLKTSCNGIRIGCPSDGEIRNCLFSNIVIEDSGNSILFE
                     YPSRYKIPEITDGANVHDIGFNNVIIHSSKRSPVAITVDEGIELERISDIYFSNIRVL
                     NGTMPVMVCGNQHVIIKNIVFNQFHLNTAAQPPIMFKYCQNIQLNQVEVSHLPNENQK
                     DK"
     gene            9083..9577
                     /locus_tag="PLW07_01515"
     CDS             9083..9577
                     /locus_tag="PLW07_01515"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOL49232.1"
                     /translation="MDRMLKVLVVTLFGFLVFAGALWAQAAKKAPDFNLTDLNGKQVT
                     LGSLKGKVVVLNFWASWCPPCKREIPDFIKTYEKFKDKGFVIVGIAVSSKPDDVKALV
                     KQYNITYPVAMASGTVEQDYGPVTAVPTTFIIDKNGNLVPGGKRIGMFSEGELEKVIE
                     PLLK"
     gene            9577..>9931
                     /locus_tag="PLW07_01520"
     CDS             9577..>9931
                     /locus_tag="PLW07_01520"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49233.1"
                     /translation="MDQHPAISKLLESKGISKEQIDLVIREKQRTGAPFGYLLIRFGL
                     MNSDSWYEFVLHGFHIPPIKIEEMQIEKDVLRVLPEFTCRKYQAIPIFRSRDKVVCGM
                     VDPLDSETVEQVKKIS"
BASE COUNT         3088 a         1751 c         2114 g         2978 t
ORIGIN      
        1 caaagaactt tggatttgcg gatgacctca ttgcaactct ttctgttgaa aaatacaaat
       61 attttgtatt tccttttcac aaaagaatcg ttgaggaatt ttctgatgga tctccaatat
      121 acatgcatct ttgcggaaat gcatggcacc tttttaaatt tttaaaagaa aatttgaatg
      181 tttattcgtt tgatactggt tttccgattg attttaccga agtcaggaaa gaactgggcg
      241 aggacgttga aatcattggc ggacccagta ttatgctgct tcaaagcggt aaagaagaag
      301 acattataaa ggaagtgaaa aagatatgtg aaagcggcat catgaaagga aaaagattta
      361 taatgagaga aggtaataac cttgctccct taacgcctgt cgaaaacata aaaagtatgt
      421 acatggcagc cagaatttat ggtcaatatt cggattgaat atactatccc gtttgtcaaa
      481 cgcaggatgg ggggcttttt atcaactaac agggcgatta cattttactt accagttcaa
      541 atatttcctg caatttcttg aaagaagggt ttgattcagt tatcatataa ataaagcccc
      601 tgtagctcag ttaggataga gcagcggttt cctaaaccgc gtgccgcctg ttcgagtcag
      661 gccaggggca cttttttatg ttaaaatagg cacaaccagg agaaaatatg aaaaaaacat
      721 ctgaatccaa tgagaaattt agaaaaggcg attcaggtcc aaaatatctt ttcagaggac
      781 ctcactgtga atggggaatt attgtcttga aaccaggtga aaagatggca gcacatgggc
      841 acaatgcagt ggtcgaagat ttcttttttc ttgaaggtag tccacacatt attgtctcaa
      901 gccagcagca aagggtattt ccaggtgatg cagtcagggt agaaccaggt gaagtccatg
      961 atatcatcaa tgacacagac caggaagtaa aacttgtctt tattaaagcg ccgtatcttc
     1021 ctgatgataa gttttcctgc tgagtacaca atggagaaag aaatggagaa agatattttt
     1081 ttaaaagcgg taaatcccct caagcaagat gttacagttg ctcttactgc aagtattcct
     1141 tgttcagaag gattgctaaa atctgcagat tccctgactc tttatgatga aaatactgta
     1201 ataccctgcc agaaaagggt acttgccaga tggaatgata gaagtattaa ggtggtgttt
     1261 attgattttg ttgcgaccct tcacacaggt acaaatatat acagaattga ttttaagggt
     1321 gatagaaaaa cagagtacat tacacaacaa tcagtaaaaa ttcgtccctg cgaagagggt
     1381 gaaaatcaag taattgttga cgacaggttt tatttgaaac tggtcttggt tgacagtaga
     1441 aatcaaaaat acagagcaaa aatcaaaaag gtcaaaaaac aaaacggcag attaacaaag
     1501 gttaccacta tctatgggtc atttgtaaca aaaacaaatg tgcggggtat tgattttact
     1561 ttgacattaa caggatgggc aggttgcaga ttgataaaaa tagagccaga aattctggtc
     1621 aatccgacag aaggagtgtt cttcagggta agagaactca aactttcatt aacaccgatt
     1681 tcaggtggta tcacagatgt attttttcct gatgttcaaa caaatcaaac acagaaagtg
     1741 aaaatgtttc aaattgacga tgaacattgt actattcaac tggataaaaa tactgaaaca
     1801 aggaacattg tctcaaccgg aaccttcatg ttgaatacag atgatcaggt catagcaggg
     1861 gcgttgaaag attttgcagt gagatggcca aaaagtatgg aatatgatgg ctattccatc
     1921 aacattgggt tgtttccttc attcaaaaga ggtacattca atcacatgca accatggtac
     1981 aagtacggtt acctttttca tggttcaaca tatctgctga aaacaggtca gaaaagaaga
     2041 tgggaaatat ggcttgatat gaatgggaac tggcagttgt gtaagtctat ttgtgagatg
     2101 ccggctgtgg ctgttgtgga cccagaatat gctatttcaa caggcgtcta tggaaacatt
     2161 tgtgcggcag ggaaaaacac tgtggattac gacagggttg tttcaaatat gtttgacctg
     2221 tacttttcag acatcaggaa aaacagggat acaggagaga tgaactgggg tgattggttt
     2281 ggagaaagat ttgtgaactg gggaaacaac gaatatgaca cagcagaaca actttttatt
     2341 cagttcataa ggacaggcga tttgagatat tttgtatctg gtgaaaatgc agcaagacat
     2401 acagatgaag tggatattat tcactctgtg aaccgtgacc ttattgaatc atttaacgaa
     2461 gaagttttca aaccatttga tgtgatgcca ggttgtgtgc acgaacatac aatagggcat
     2521 gttggtggtt tttgttcaag gaaaaaggtg aaaaagatgt tgagcaaggt gctgggagtg
     2581 aaaaatcctt acctctgtct tgatccctgg aacatgggac acctgtggct tgaggggttg
     2641 agagacgcgt attttttcac aggggatgag aaatttaaac agacggtgat ccttgttgct
     2701 gattacctct caaaagttgt tgcagaaaga ctctaccctt tttccgggca tgctcattct
     2761 ggtagaactg ttggatggcc actgactgcg ctctgtgcag catacgacat taaaaaagat
     2821 aggaaatatc tcaaagcaat gaagatactt gtcaatgata tgcttgctga acaggaccct
     2881 cattgcggtg gttggcttta cgaattgcct gatggtcatt gtaattgtgt caggaaaaaa
     2941 catgtgggca gcgcaggttt tttaatcagt atccttgtga atggattgag ccgatattat
     3001 gaaatgacag gcgataaaag gattccacag gcagtgaaaa aagcaatgga attccttgta
     3061 gttgatacat gggatgaaag gacaggtaag tggaggtata catcatgtcc ggtatcccct
     3121 tttgtggaac cttatggatt gattttgctt tcactggcaa acggcataaa cattggaaat
     3181 gttgatgtca gggatgtttt cgtaaaaaac tggcagcatt tcgtggaagg ttgtgataaa
     3241 gatattaatc aaaaaatgat tggtagactt ttttcaattc gtcttttcgg tacagcaaag
     3301 gcattgaaaa tttatcacca gataaaaagc aatgaataga aaatggctgt atgtgattac
     3361 ccaggatgtt tcaccacaaa tgaatatggc acttgatagt atcatgtgga ataaagtgaa
     3421 acaatatcct gacactgcga tattgagatt ttatacatgg aaaccatcag gtgtttctct
     3481 tggagcacat cagaaaccag aaaatcttgt taacgttagt ttttgtaagc agaacaatat
     3541 ccctgtggtt gcaagaatta ctggtggaag tgcaattttt catgacaggg aaatcacata
     3601 cagtttttca gcaacaaatg atgagaaggt tttttcagga cccataacat catacgaaaa
     3661 aatatgcggc gcattgaaaa ctgggcttga aaaactcggt atcagggttc aatggcgcgg
     3721 ggtttctaaa ggaaaagaac cttcttttac tgacagggac tgtttttctc tctcaacaag
     3781 gcatgacctt gtggtagatg acagaaaaat tatcggtagc gcgcaaagga aggacagaac
     3841 atcttttttg cagcatggaa gtttattgat tgaaattcaa aaacacctgt gggaaaaaat
     3901 atttcttgca aaacccgatt tttcaaagat tatatcaatg tctgaaattt taagtaatgt
     3961 gcctgatttt gatacactga taaaagtgct gaaaaatggt ttcgaacaat tttttgaaag
     4021 tgattttgaa gaaatacatt ttaccagcga agatataaaa gaggcagaaa aaattcaaca
     4081 gacattttta cttgtttgaa agatgaaaaa ctttttaata accctgactt ttttcgttgt
     4141 tacatgtgtc ttttcgcagc agaaacaagg atggaaatga gtcaatacag gtgggtaaaa
     4201 atcacagatc atgcggcgtt tgcaccaaga gatggagcag gtgctctggt attcaaaaga
     4261 aaaatgtggc ttctgggagg atggaatcct tctgataaaa ttcactttcc gaaaatctgc
     4321 aatagtgagg tgtggtcttc aaaagatggg ataaactgga aactggaaaa tccctgtgca
     4381 ccatgggaag gtcgacatac tgctggatat gtggtgttta acgatgcaat gtggattgta
     4441 ggaggagatg ccaatcagca tcattatcag aatgatgttt ggaaaagtac agacggcgta
     4501 aaatgggaat gtgtgtgcga aaatgtgccg tggggactca gggttctcca ttacacagtt
     4561 gctttcagga ataaaatctg ggttattggt ggacagaccc ttccacactt tgcgccagca
     4621 gatgaaattt tttattcaga tgtatggtgc agtgacgatg gaagacactg ggaaaaggtt
     4681 gctgataacc ttccatgggc tccaagagga atgataggag gaagcgcagt attcaaggat
     4741 aaaatctgga ttctgggtgg tggaacatat gatactccca aaaaaccaga gagaattttt
     4801 tatcatgatg tctggtgctc acaggacgga aaaaactggg aactggtctg tgatagtgca
     4861 ccctggcatc cacgacagta ccatgatgtt gcagtgtttg ataacaaact ctgggtcatg
     4921 gaagggtatc ataaaaatgg tggtaataga aaggacgtct ggtattcaga ggacggaaaa
     4981 aactggactg aagtttctga tactccatgg caaccaaggc atgcagcaag cgtatttgtt
     5041 tatgataatg ggttgtggat ggttgcaggt aataacatga cacctgatgt atggaaactg
     5101 gaaaagataa ttaagtagaa tgcattctat ctttcccatt ttatatgact gtttataccg
     5161 aatttttcgt atgttttcag tacttcatac tagttctgtg tttttttctg ctttcgctag
     5221 ctcgtacaaa cggataaaca tcttcttacc tgtctctgtt tgcttcaatg ttttcttaat
     5281 gaaattatgc tgtgcacaca atgtctgacc gttttcaatc gcagacgtgc cacccaggta
     5341 tctgggcttg atatggtcga cgtgaagctc aacaccgtct tttattcctc taccacagat
     5401 gacacatcgg tatccgtctc tctttaaaat tgcttctttc tgttcttgag taaactcctc
     5461 taattctatt tttttcactg cagaagggtc atatttatat actccctttc gaactttaat
     5521 cagaaaaccc gcctcatgta aattcctgat agccctccat gtatcacggg gtttcttccc
     5581 ataaagtcgc acatattttc cttcaaccca atcaaccaca ggtccatgcg gcatgtcttc
     5641 attcggatgt gcttcaaaaa attcgatcag caggtctttt attgtttttc gtttcggcat
     5701 agtattaaca aacagtgatt aactgcccct ggtctatatt tgcttccttt ctcagtcttt
     5761 ttatagccag ttcacaatag tttttatcca cttcaacgcc tattccttta cgattattaa
     5821 ggtatgcagc aataagtgtg gtaccgcttc cgaggaatgg gtccagaaca gtatctccaa
     5881 caaaagtgaa cagttttata caacgctttg gtagttcaac aggaaatgga gctggatgac
     5941 ctattttttt cttactttct ccagaaaagg tccaaagacc atttgtccat gacataaatt
     6001 cctttttagt aatgtcggaa actcttgttc cgcctgtttt tttccattta tttttgtaca
     6061 ttactacaat gatttctaca ggtgcaataa cataaggtgc tgaagcagac ataaacgaac
     6121 cccaggcagt acgacgtgag atatttcctt cgttccatat aattgttgag tgatatatga
     6181 aaccgatttt tttgcgatcg tcgtaatatc cgccccaacg ctttgttgac ctcctttgtt
     6241 tttatcaaga ggtatgttta aacagattct accatcatcc ttcagtaccg aataagcttt
     6301 tttcagccat tgttcagtaa aatcaagata attgtcatac gagattgtat catcgtaaaa
     6361 attataatga atatcaacgt tatatggcgg agaagttaca attaagtcga tagaacattc
     6421 cttaattttc ctggttgata aaaaattttc gttaattatt acgatcgatt ctgactgaaa
     6481 gtacgccgta ctatcctcgc ttactttgtt tgctacagcc gttttattgt attttttggg
     6541 ttttttcatt ggactataaa agtttcatgt ttctttgttt cataaatatt acacaaataa
     6601 atatgtgacg tcaataaaga aagtttgatt atgcttcttg tgaaataagt atctgggata
     6661 taatacaatg gaactgataa acaaggaaat ctggagggaa aagatgacag caaaagaaag
     6721 atggattgca tcattgaagc ttgagccagt tgataaactt gccttctggc caaaaattag
     6781 tggttcctac ctcaattacc agggtggcag tttcaaaagt atgactgttg aacagatgca
     6841 tgattacatt ggtagcgaca aacaccagtg gctggtgggt ttcataaaag agaagagaag
     6901 aacgacttcc tttgaagaga taaaacaggg tatgacaaga aaacttgttt attcaacaaa
     6961 gtatggaaac cttactgcta ccatgcgatt tgatgaacca tctcaggcgt ggcatccagt
     7021 tgaatatcct gtaaaggcaa gacaggatat tgaaatcatg acagaatggt ttgcagatgc
     7081 ctgctttgaa atagatgagg aaaaattgca ggaagcaaag caaatgtaca aagaaactgg
     7141 ttctgatgca attacagccc attccatcgg aaccacacct ttaatgcagt gggttcagca
     7201 tcttgctggc attgaaaact gtcattattt cctgagtgat tttacagaag aggttgaacg
     7261 attgtttgaa accatgcaga agaaaaatcg taaatctgct gaaatccttg cgcaagacag
     7321 tcctgctgaa attttgtatc ttgttgaaaa cacttccaca accctgattt ctccacagca
     7381 gtacaggaaa tactgctata aggatatcct tgattgcgca ggtattgtaa atgactcagg
     7441 aaagtttctt gttcttcata tgtgcgggca cctgaaaaat ctgctggttg atcttgcaag
     7501 gttacctgtc tctggctttg aagcattcac cacaatgcct gttggcaata caacacttgt
     7561 tgaggcaaga aaaatatgtc ctgataagtg tttgattggt ggaacaaatg cagtactctg
     7621 gacaaaatca gcagaagaaa ttatcaatga gattgaagaa gaactttata aattgccaca
     7681 tcacagagga cttgtcatta cttctgcggg tgtcatgccg cctgtgtgta agccagaaac
     7741 aataagacag gtctgtcagt gggtgcaaca atacaggata agagtttaaa catcaatctg
     7801 caggttattt atctttttga ttttcattcg gcaaatgcga cacttccacc tgattcagtt
     7861 gtatattctg gcagtactta aacataatag gtggttgtgc tgctgtgttc aagtgaaatt
     7921 gattaaatac aatgtttttg ataattacat gttgattgcc acagaccatt acgggcattg
     7981 tgccattgag aaccctgata tttgaaaaat atatgtctga aattctttcc aactctatgc
     8041 cttcatctac agtaattgca acaggggacc ttttagaaga atgtatgatc acattgttaa
     8101 acccgatatc atgcacgttt gctccatctg taatttcagg gattttatat cttgaaggat
     8161 attcaaataa aatgctattt ccactatctt caatcacaat gtttgaaaaa agacaatttc
     8221 tgatttcacc atcagacgga cacccgatac ggatgccatt acaactggtt ttcaaaacac
     8281 aatttgtcac tgtaatattt tcacacggac agtactcttt ataaaaatgc ttcatattac
     8341 gcactacaat gcaatcgtct tctgtgtcaa aaatacaatc actcacagtg acatttttgc
     8401 aggcatctat gtcaatgcca tcaatatttc tcagccgtct atcaccagaa acttttattc
     8461 tgtggatgca aacattttca cattgcatca gccatattgt ccagcacgcg gaatccacaa
     8521 aaccaatgtc ttcaatcagt acattcttac acctgtaaaa cattatgatc cttggccgct
     8581 gtgtttctgg cttttcaaat ttgccgtttg cgtcaacttt tttatggtca taaaaagcag
     8641 gacctgcccc attgattgtt cctgcgcctc tgatggatat gttttcagca tatgaggcac
     8701 aaattaaagc attgttactt ttctctggag gatttttccc tatgaagggc tcttccaggt
     8761 tttgataatc agaaagattt tcactgcctt tgaggacaga gccggcttca agaaagagtt
     8821 caacattgct tttcaatatc aatgttccgg ttatgaaaac gccgctatcg caaatgaccc
     8881 tgccaccgtt ttttttaaaa caactgtcaa ttgctttctg gatggatttg gtacttactg
     8941 tgctggtatt cggcacagca ccatattctg taattttgaa ttgttccatg tattgctcct
     9001 tttgagcaga tgttaacctt tttctgtttt ttaaccgtca taatattgat ggtataatac
     9061 catatcacaa aaagggggga taatggatag aatgttgaag gtgctggtgg taacattgtt
     9121 cggttttctg gtgtttgctg gtgcgctctg ggctcaggca gcaaaaaagg cgcctgattt
     9181 taatcttact gacctgaacg gcaaacaggt tacactgggt tcgcttaagg gaaaggttgt
     9241 tgttctgaat ttctgggcat catggtgtcc accatgcaaa agggaaatcc cagattttat
     9301 caagacatac gaaaaattca aagataaagg gtttgtgatt gtgggtattg ctgtcagcag
     9361 caaacctgat gatgtgaaag cgcttgtgaa acagtataac atcacctatc ctgttgcaat
     9421 ggcttctggt acggttgaac aggactatgg cccggttacg gcagttccaa caactttcat
     9481 tattgataag aatggtaacc ttgttccagg cggaaaaagg ataggtatgt tttctgaggg
     9541 cgaacttgaa aaagtcattg aacctttgct gaaataatgg accagcatcc agcaataagc
     9601 aaacttcttg aatcaaaggg tatatcaaaa gaacagattg acctggtaat cagggaaaaa
     9661 cagagaacag gcgctccatt tggatacctg ctaatcagat tcggactgat gaattcagat
     9721 tcatggtatg agtttgtcct tcatggtttt catatcccgc ccataaaaat tgaagaaatg
     9781 cagattgaaa aggatgttct gcgggtactg cctgaattca cctgcagaaa atatcaggca
     9841 atccccattt tcaggtccag agataaagtc gtttgcggaa tggttgatcc tcttgattct
     9901 gaaacagttg aacaggtcaa aaaaatttct g
//
LOCUS       DAORYU010000025         9463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_5409_length_9463_cov_4.157207, whole genome shotgun sequence.
ACCESSION   DAORYU010000025 DAORYU010000000
VERSION     DAORYU010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9463
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5409_length_9463_cov_4.157207"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4591)
                     /locus_tag="PLW07_01525"
     CDS             complement(<1..4591)
                     /locus_tag="PLW07_01525"
                     /inference="COORDINATES: protein motif:HMM:NF020121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HOL49234.1"
                     /translation="MDKKKSGVIIYFLYCLFFVAFCSAQKQPAKMNFSILHSSDTGGA
                     NIFCVAADPVKKKLYAGTYSYDPERKGLLVYDLNDNGTVKGQPRKYSDHPDPMPDPLP
                     PGGWSSVTAIFIDKKHNKLFLGVSVAGVKGFENFTKSLVMYQLNEDGEPVGRPVAFES
                     GNPHKHIAAITMHPKFNRLYLVGWGGSGVYAIDLDEKGNPVSQPVAYNLLGHGKYSIH
                     FNNEGTKVYLGTYPSVLQVCDVDSNGAIGNARSYSVKDGPQEYMICAVTERAVFFKGP
                     DNNLSYYLLDSSGNIQADCVVVGNFPVQAVTNSDVPNRVIVAASSNFTDAITGKTISD
                     GFTLKEIELSPDGKINVVRESEKFLREKALCINGFPSITVATTHLGWGFLGNRMKGLE
                     IRTTLKSIDSDSKLPIAFKTITIGPEQKYFKFIYSQKFNKIYSAGDNHIYVYSPKDHT
                     LVSVLCPELQGPIAIDEASNIVYAARKDGTIAIRTLNSSGIPASEGENLQSGVKPITL
                     LLFNSQAKVLYVIGSLQKQEPESPVFCRIPGLTYDIAAVVDATRGKLYIASQYNRNQN
                     LTIWDIDKGGKTVSETPKRFPDGFISETKRSLIYGMIVDTKRSKLYLSGMLENPGKNE
                     AGIMVFELDAKGEPQGNPKFYPTRSRTNSPWDICLSLDRQQLYEVGWGVPQVFVWNLD
                     ENGLLQGEPDVWETTGNGKSQIFPLPDRSAILLGTYPSILEVVPLDKKGNVVGGVQMA
                     LSTDKGRINLGFMGAGFSNDWVNLDTQLKDATGTARIICEMSGAPVKKALVEFEFRKA
                     DGSNIQNLKSFTIETRGNTISIFVPNYGLDQPEKFTDFIQSSIDKFKQYLAYAKKYAL
                     KDEEKPKEIIVANGLISLDGTQQTLEDGLNILSMLGHNTIQIWQWGNIPPEEIRQKAA
                     KYGFNRFRGAVYNPPSYFDYNTSMVTDQYLDNWLNGLKKSFSNMGQKNEEMILFHMAD
                     EPGWYYPHQIDEVKKDPSRLDVFRNYLKSKRFTPQTFGKNSWDDVYPMKLSEPVNLAD
                     RKLFFWTTRFYVESLSNAFAAATRAFQRAYNSNILTTTNLNNWPGLFFRPSPGQKIAN
                     NWDAGPDAGMGLPDWFDLGRKKAVSCIWTEDWFGDSSAQNWSMYGDLLRCAAREGGIE
                     YGGYIVGHTTGGRVPEGGKYKIMALIGHGAKAIDPYIFGPGPAFADGWSEREQVYQSL
                     SEGIRILGKSEKLVAPGRPKNGTVAILLPQASQVWDSDANTKAYIAELHGLHEALTHE
                     NYPVDFVDDFDLEDGILTKNNYAVLYVTAPNLSDKAQKNIAEWVNAGGCLVLSPGACN
                     YDQYNEPLVTISRLTGIKPVSVARIACPHSWELERLKKTTIFVKDARFETEQVPAQLQ
                     VISLPPVTGKTLAVLEDGSSAIVENSYGKGKIFSLGFWPGITYHNSPDRSDYKKLPQG
                     WSESARKIITTFAKIAGAEKFVEISEDLVEGCYLESEKGIAVVLLNWSGQPKKEITVK
                     VNTTKTISKIESVEHGILKFDKAQNSVTVTLPLKTV"
     gene            complement(4658..5233)
                     /locus_tag="PLW07_01530"
     CDS             complement(4658..5233)
                     /locus_tag="PLW07_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015225650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49235.1"
                     /translation="MSTRAVGDAIQHILEENFPTPIGDVSKNYSAGFARRAMADLAFQ
                     DQNGLYYIVDVKTHRLETKFNMPNLTSVERLARFYEDDANYYVVLMVTYSIVREVDIC
                     VKKVNFVPIEFLSWKCLTIGALGWGQIQIANANKIDVIPGYSRKRWMLQLCENLFEFY
                     PLEIEKVKVRMAYFRKIRTQWEQKEDIWKQE"
     gene            complement(5335..6336)
                     /locus_tag="PLW07_01535"
     CDS             complement(5335..6336)
                     /locus_tag="PLW07_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015169235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOL49236.1"
                     /translation="MEKALRNRSIALSNTERERYRLELLRTNKVVTLKEIINKIINQD
                     MYEVLDFLPDNFVDLLFVDPPYNLTKSFNNRSFKQMHVFEYMEWMDSWLKKLIRLLKP
                     TASIYICGDWRSSSAIHLLCEKYFIVRNRITFEREKGRGAMKNWKNNSEDIWFCTVSN
                     QYSFNAEKVKLKRRVIAPYRDNGKPKDWTEEENEKYRLTYPSNIWTDITIPFWSMPEN
                     TPHPTQKPEKLLAKIILASSFENDIVFDPFAGVGTTGVVAKKLKRNFVMIEIDETYCL
                     YGIKRLHMAEQDSTIQGYVDGVFWERNTLKDMKNADGKRKHTTNGNLSLFLNTGQNK"
     gene            complement(6525..8357)
                     /locus_tag="PLW07_01540"
     CDS             complement(6525..8357)
                     /locus_tag="PLW07_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49237.1"
                     /translation="MRRDFILEALVDFPDDALSAPCPLTGVHIDNLMKLLASHGIKRV
                     IWGYYGDGHGGFLMPGIENGPDLLYDMNQYGYYAKTIEILENPLQVATEAAHRCGMEI
                     YAYFKPYETGVSVDFPEGSIQARLWGRLYRIGGYLSWIDPFVVKNPHLRIKRRTDDLW
                     YGIENEVITTIKLTKKDDSPTRIRKENIEIWTSNLNYKYRKKDIDFSFKEVIEPSKKD
                     VYDVYGNLVTRKGQPVRILFLSNFALTNKYILITTNFQSTDADFAHTWDCLFTAYNEK
                     GVEIPGVYATGTAIWFPEWENFRNCGVSFDTGRGPELIYLDLPNNPQDDQIRVEGAKK
                     FHLKGQSKAQGFIAFSRGRNLYLPGGLCETEPAVQEYWLRCIGEMIDAGVDGVEFRVE
                     NHSCHTDTPQDYGFNDIVLEKIDVHNDTLAEITRVRTQAYSAFLKKASHLIRTNGKKV
                     RINLNVDWFRDPSQRPGRRKLAFPANIDFEWENWIDQHIMDQAVLRIFAKDFSGIFGK
                     DDVASKMIEKCYQNKIPLTVSRYVWNNDGLMDEFIKVYENEKFNGFILYETWSFIEFE
                     KNGNCRISFPEMMPVVNMPDQLWNAKSNTGKNVKKILTYFNNTL"
     gene            complement(8367..>9463)
                     /locus_tag="PLW07_01545"
     CDS             complement(8367..>9463)
                     /locus_tag="PLW07_01545"
                     /inference="COORDINATES: protein motif:HMM:NF024757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta galactosidase jelly roll domain-containing
                     protein"
                     /protein_id="HOL49238.1"
                     /translation="HMHDMPLYKKWGVVGFSNEGRTIRIESGIFPTYLYARMMWDADL
                     DAKALMDEFFTNWYGPAAQPAKAFWEELEITLEKTPWLGHEDRILPYVYSDQLIEKLE
                     KYIKQAESLAKEKVHQEHVFADRIVLDNLKAFMEMHKAEWKCDFAEAAKQAQRMLDVR
                     AQASKLSRFYFDPNPARGGDDVGYYYWGAAARAKYYQQLADMTKGVTGEMIVVLPEKA
                     KFSLDPRDDGRYMGWYIPAFDDSKWTTISTTIPFYGQGYCDSQGYPYMGVIWYRLNVN
                     VPSSAKGKKIFLYAPAVETEAWVWVNGKLVGHRPYRDAYERPNEINMDVTDAIIPGKT
                     NLIAVRVHTGFNAAQQSAGLTSRLFLYSPK"
BASE COUNT         2551 a         1986 c         1703 g         3223 t
ORIGIN      
        1 ccactgtctt caatggcaat gtaactgtga cactattttg tgccttatca aacttcaata
       61 taccatgttc aactgattca atttttgaaa tcgtttttgt tgtattgact ttcactgtga
      121 tttctttttt cggttgacct gaccagttta aaagaacaac tgcaatgcct ttttcacttt
      181 caagatagca tccttcaacc aggtcttctg agatttcaac aaatttttct gcaccagcaa
      241 tttttgcaaa tgtagtaatg atttttcttg ctgactctga ccatccctgc ggaagttttt
      301 tgtagtcaga cctgtcagga ctgttgtgat aagtgatacc tggccagaat cccaatgaaa
      361 agatttttcc cttaccataa gaattttcaa cgatagcaga agaaccatct tcaagtacag
      421 caagtgtttt tcctgtcaca ggtggcagag aaattacctg taattgtgca ggaacctgtt
      481 cggtttcaaa tcttgcatcc ttaacaaaga ttgttgtttt tttcaatctc tcaagttccc
      541 aggaatgtgg acatgcaatt cttgcaacag acactggttt gattcctgtc agtcgtgaaa
      601 tggtgaccag tggttcatta tactggtcat aattgcatgc tcctggtgaa agaacaaggc
      661 atccaccagc attgacccat tcagcgatat ttttctgtgc cttatcagag aggtttggtg
      721 ctgtgacata aaggacggca taattgtttt tcgtcagaat accatcttca agatcgaaat
      781 catcaacaaa gtcaacagga taattttcgt gtgtaagtgc ttcgtgcaat ccgtgtaatt
      841 ctgcaatata tgctttggtg tttgcgtcag aatcccagac ctggcttgcc tgtggcagaa
      901 gtattgcgac tgttccattt ttgggtcttc ctggggcaac caatttttca cttttcccga
      961 gaattcttat tccttctgac agcgattgat atacctgttc tctttctgac catccatcag
     1021 caaaggcagg acctggtccg aaaatgtatg ggtctattgc ctttgcgcca tgtccgataa
     1081 gcgccataat cttgtacttt ccaccttctg gaaccctgcc gccagttgta tgtccaacaa
     1141 tgtagccacc gtattcaata ccgccttcgc gcgctgcaca ccttaaaagg tctccgtaca
     1201 ttgaccagtt ctgcgcagaa ctatctccga accagtcctc tgtccagata caggacactg
     1261 cttttttcct gccaaggtca aaccagtcag gaagacccat tccagcatca ggaccagcat
     1321 cccagttgtt tgcaattttt tgtccaggag aaggtctgaa aaataatcct ggccagttgt
     1381 tgagatttgt tgtggtaaga atgtttgaat tgtatgccct ctggaatgcc cttgttgctg
     1441 ctgcaaaggc attgctcaat gattctacat aaaaacgtgt tgtccagaaa aacaatttcc
     1501 tgtcagcaag attaacaggt tcactcagtt tcatgggata tacgtcatcc caggaattct
     1561 ttccaaatgt ctggggtgta aatctttttg atttaaggta gtttctaaat acatcaagtc
     1621 gtgaaggatc tttttttact tcatcaatct ggtgagggta ataccagcca ggttcatcag
     1681 ccatatggaa gagtatcatt tcttcatttt tctgtcccat atttgagaaa ctttttttca
     1741 agccatttaa ccagttatca agatattggt cagtcaccat tgaagtattg tagtcaaaat
     1801 aggaaggtgg attgtacact gccccgcgaa atctattaaa cccgtatttt gctgccttct
     1861 gtcttatttc ttctggtggg atatttcccc attgccatat ttgaatggtg ttgtgtccga
     1921 gcattgaaag aatgtttaat ccatcttcca gtgtttgttg ggttccatca aggctgatta
     1981 aaccatttgc aacaataatt tcttttggtt tttcttcatc cttcaatgca tacttttttg
     2041 cgtatgcaag gtattgtttg aatttgtcaa tactggactg aatgaaatca gtaaattttt
     2101 ctggctggtc aagaccgtaa ttgggtacaa aaatactgat ggtatttccg cgtgtttcaa
     2161 tggtaaaact tttcaaattt tgaatgtttg agccatctgc ttttctgaat tcaaattcaa
     2221 caagcgcctt ttttactggt gcgcctgaca tttcgcagat aatccttgct gttcctgttg
     2281 catcttttaa ctgggtatca agatttaccc agtcgtttga aaatccagca cccataaatc
     2341 ctaaattgat tctgccttta tctgtggaaa gagccatctg aaccccacca acaacatttc
     2401 cttttttatc cagaggaaca acttcaagaa tggatggata tgtaccaaga aggattgcac
     2461 ttctatcagg taaaggaaaa atctgagatt ttccgtttcc agtggtttcc cacacatcag
     2521 gttcaccctg taacagtcca ttttcatcaa gattccacac aaatacctgc ggtactcccc
     2581 agccaacttc gtacaactgc tgcctgtcaa gggataaaca tatgtcccat gggctgtttg
     2641 ttctgcttct tgtgggataa aatttcggat ttccctgtgg ttctcctttt gcatcaagtt
     2701 caaagaccat aatgcctgct tcatttttcc ctggattttc aagcattccg gatagataca
     2761 gttttgacct ttttgtatca acaatcattc cgtaaatcaa acttcttttt gtttcagaga
     2821 taaatccatc aggaaatcgt tttggtgttt ctgaaactgt ttttccaccc ttatcaatgt
     2881 cccatattgt gagattttga tttctgttgt actggcttgc aatgtataat tttcctctgg
     2941 ttgcatcaac tacagcggca atgtcatagg taagtccagg tatacggcag aatactggac
     3001 tttctggttc ctgtttttgt agagagccaa tgacataaag cacttttgcc tggctattga
     3061 aaagaaggag ggtaattggt ttgacacctg attgaaggtt ttcgccttca gaagcaggga
     3121 ttccagaact gttcagtgtt cttatggcaa tcgttccatc ttttcttgca gcataaacga
     3181 tgtttgatgc ttcatcaatg gcaatgggtc cctgcagttc agggcataac acagaaacaa
     3241 gtgtgtggtc ttttggtgaa tacacataga tgtgattgtc accagcagag taaattttgt
     3301 tgaatttttg tgagtaaata aacttgaaat acttctgttc aggaccaatc gtgattgttt
     3361 tgaacgctat tggtagtttt gaatctgagt caatagattt taaagtggtt cttatttcta
     3421 atcctttcat tctgtttcca agaaatcccc atccaaggtg ggttgttgcc acagtgatcg
     3481 atggaaaacc attgatacac agcgcttttt ctctcaaaaa tttttcagat tcccttacaa
     3541 cattgatttt tccatcagga ctcagttcaa tttctttaag agtaaatcca tcagaaattg
     3601 tttttcctgt gatggcatca gtaaaatttg aagacgctgc aacaatgacc ctgtttggta
     3661 catcagagtt tgtgactgcc tgtacaggaa aatttccaac aacaacacaa tcggcctgga
     3721 tgttgccgga actatcaagc agataatatg agagattatt gtctggaccc ttaaaaaata
     3781 ctgccctttc agtcactgca catatcatgt attcctgtgg tccatctttt acagaatatg
     3841 agcgtgcatt tcctattgcg ccatttgagt caacatcaca tacctgaagc acagatggat
     3901 atgtcccaag atatactttt gtcccctcat tattaaagtg aatggagtac ttgccgtgtc
     3961 ctagtaaatt ataagccact ggttgggata caggattgcc tttttcatca aggtctattg
     4021 catagacacc agaaccaccc catcctacaa gatataatcg attgaatttt gggtgcattg
     4081 tgattgctgc gatgtgtttg tggggatttc cactttcaaa agccacaggt cttcctactg
     4141 gttcaccatc ttcattcagt tgatacatca caagggactt tgtgaaattt tcaaatccct
     4201 ttacgcctgc cacacttaca ccaagaaaaa gtttattatg ctttttatca atgaaaattg
     4261 ctgttactga actccatcca ccaggtggaa gtgggtctgg catcggatca gggtggtcag
     4321 aatattttct tggttgacct tttacagtgc cattgtcatt caaatcataa acaagaagcc
     4381 cttttctttc agggtcatag gaatatgttc ctgcatacag tttctttttt acagggtctg
     4441 cagccacaca gaatatattt gctccacctg tgtcagaaga atgcaggata ctaaaattca
     4501 tttttgctgg ctgtttctgt gcagaacaaa aagcaacgaa aaatagacaa tataaaaaat
     4561 aaataatcac gcctgatttc tttttgtcca tatggtctcc aatgttaacg aacttctaat
     4621 gtgattataa tttgagtcgg aggtgcccat taaaaaccta ctcttgtttc cagatgtctt
     4681 ccttttgttc ccattgtgtc ctgatttttc taaaatatgc cattcttact tttacttttt
     4741 ctatttccag gggatagaat tcaaaaagat tttcacacaa ttgaagcatc cacctttttc
     4801 gtgagtaacc ggggattaca tcaattttat tggcattagc aatttgtatc tggccccacc
     4861 ccagggctcc gatggtcaaa cacttccagg atagaaattc aatcggaaca aaattaactt
     4921 tcttgacgca aatatccact tctctaacaa tgctgtaagt taccataagt acaacatagt
     4981 agttggcatc atcttcatag aaccttgcca gtctttcaac agaagttaag ttgggcatgt
     5041 taaatttcgt ttccaatctg tgggttttta cgtcaacaat gtaatagaga ccgttttggt
     5101 cctggaaagc aaggtcagcc attgctcgcc ttgcaaaacc cgctgaataa ttttttgaaa
     5161 catctccaat aggtgttgga aaattttctt ccagaatatg ctgaatcgca tctcctactg
     5221 cccttgtact cattacagtg tctctgctta aaaatcttgg gtatctgtta agaaagtttt
     5281 ttattttatt ctgaattttt ttgtactcag aggtattgta gaatattttg cttatcactt
     5341 gttttgtcct gtgttcagaa aaagggataa atttccatta gtagtatgtt ttcttttacc
     5401 gtcagcattt ttcatatcct tcaaagtgtt tctttcccag aaaacgccat ccacatagcc
     5461 ttgaatagtt gagtcttgtt ctgccatgtg taatctttta attccataaa gacagtatgt
     5521 ttcatctatt tcaatcatca caaaatttct tttcagtttt tttgcaacaa cacctgttgt
     5581 cccgacacca gcaaatgggt cgaatacaat atcgttctca aaagaacttg caaggataat
     5641 tttcgcaaga agtttttcgg gtttctgtgt tggatgaggt gtattttctg gcatggacca
     5701 gaaaggaatc gtaatatctg tccatatatt tgatggatag gtcaatctat atttttcatt
     5761 ttcttcttcg gtccagtctt ttggtttacc attatcgcga tatggtgcaa tcactcttct
     5821 ttttaacttc accttttccg cattgaaaga gtattgattt gaaacagtgc agaaccatat
     5881 atcttcgcta ttgtttttcc aatttttcat tgcgccgcgc cctttttccc tttcaaacgt
     5941 aattctgttt cttacaataa aatatttctc gcaaagcaaa tgaatagctg aagaagacct
     6001 ccagtctcca catatgtaga ttgaagcagt tggtttcaaa agacgtataa gtttttttaa
     6061 ccaggaatcc atccattcca tatattcaaa aacatgcatt tgtttgaaac tacgattatt
     6121 aaaagatttt gttaaattgt atgggggatc tacgaacaaa aggtcgacaa aattatctgg
     6181 aagaaaatca agtacttcat acatgtcctg gttaatgatt ttgttaatta tttctttgag
     6241 agtaacaact ttattagttc gcaatagttc caaacggtat ctttcccttt ctgtatttga
     6301 tagagcaata ctgcgatttc gcagtgcttt ttccatatat gttttccttt aatacaaaac
     6361 accttaaatt ttcagtttca tatgggcact ttcattatat taaattcttt cttatgttaa
     6421 tctcaaagta acatattttc gattgtcttg caatatcgct ttgcattatt gttgatgttg
     6481 cccgaccata aatattttca aatgacaatt tgtgccatcg ttttttataa tgtgttattg
     6541 aaataagtga gaattttttt gacatttttt cctgtgtttg attttgcatt ccatagttga
     6601 tctggcatat tgacaacagg catcatttct ggaaaagaaa ttctgcaatt tccatttttt
     6661 tcaaattcaa tgaaagacca tgtttcgtaa agaatgaaac cattgaattt ctcgttttca
     6721 tacactttta taaattcatc cataagtcca tcattgttcc atacatacct gctcactgtc
     6781 aggggaattt tgttctgata acatttttca atcattttac tggcaacatc atccttgcca
     6841 aaaatccctg aaaaatcttt tgcgaaaatt cttaatactg cctggtccat aatatgctgg
     6901 tcaatccaat tttcccattc aaaatcaatg tttgcaggga atgccagttt tcttctgcca
     6961 ggtctttgag atgggtctct gaaccagtca acattgagat tgattctgac ttttttccca
     7021 tttgttcgga tcagatggct tgctttcttc aaaaatgctg aatatgcctg agttctcacc
     7081 ctggttattt cagcaagggt atcattgtgg acatctattt tttcaagcac aatgtcattg
     7141 aagccataat cctgtggagt atctgtatgg caggagtgat tctcaaccct gaattctacg
     7201 ccatcaacac cagcatcaat catttcaccg atacatcgta accagtattc ttgtacagca
     7261 ggctcagttt cgcataaccc accaggaaga tataagtttc ttccacggct gaatgcaata
     7321 aatccctggg ctttgctctg acctttcaga tggaattttt ttgcaccttc cacacgtatt
     7381 tggtcgtcct gcgggttatt aggcaggtca agatatatca gttcaggacc ccttccagta
     7441 tcaaaactga ctccgcagtt acgaaagttt tcccattcag gaaaccagat tgcagtacct
     7501 gttgcataaa ctccaggaat ttccacacct ttctcattat atgcagtgaa aagacaatcc
     7561 catgtatgtg caaagtctgc gtctgttgat tggaaatttg ttgtgataag aatatatttg
     7621 tttgtgagcg cgaagtttga aagaaacagg attcttactg gttggccttt gcgtgtgaca
     7681 agatttccat atacatcata aacatctttt tttgacggtt cgatgacttc cttgaaactg
     7741 aaatcaatat cttttttcct gtatttgtag ttgagattgc ttgtccagat ttcaatgttt
     7801 tcttttctta ttcttgttgg agaatcatcc ttttttgtca attttatcgt ggtgatgact
     7861 tcgttttcaa taccatacca caagtcgtcg gttcgtcttt ttattctgag atgcggattc
     7921 tttacaacaa atgggtcaat ccatgagaga tacccgccaa ttctatagag acgtccccat
     7981 aatcttgcct ggatagagcc ttctggaaaa tcgacagaca cacctgtttc ataaggtttg
     8041 aaatacgcat agatttccat gccacacctg tgagctgctt ctgttgcgac ctgaagcgga
     8101 ttttcaagaa tttcaatggt ctttgcgtaa tagccgtact gattcatgtc ataaagaagg
     8161 tcaggcccat tttcaatgcc tggcattaaa aagccaccat gaccatctcc ataataaccc
     8221 cagatgactc ttttaatacc atggctggca agcaatttca ttaaattatc aatatgaaca
     8281 cctgttaaag ggcagggtgc actcagtgca tcgtcaggaa aatcaacaag cgcctcaaga
     8341 ataaaatccc tcctcaataa acctccttat tttggtgaat acaggaataa tcttgatgta
     8401 agacctgctg attgttgagc agcattaaac ccagtatgaa cccgcacagc aataaggttt
     8461 gttttacctg gaataatggc gtctgtaaca tccatattga tttcatttgg tctttcatag
     8521 gcatccctgt aaggtctgtg tcccacaagt tttccattca cccataccca tgcttctgtt
     8581 tcaactgctg gcgcataaag gaaaattttt ttaccctttg ctgaagatgg tacattgacg
     8641 ttgagccggt accagattac acccatatag ggatatccct gactgtcaca atatccctga
     8701 ccatagaacg gaattgtggt ggaaatggtt gtccacttag aatcatcaaa tgctgggata
     8761 taccagccca tatatcttcc atcgtccctt ggatcgagag aaaactttgc tttttcagga
     8821 agaacaacaa tcatttctcc tgtcacgcct tttgtcatat cagcaagttg ctgatagtat
     8881 tttgcccttg ctgctgctcc ccagtaatag taaccaacat cgtctccgcc ccttgcagga
     8941 ttcggatcaa aatagaatct actcaatttt gatgcctgtg ctctgacatc aagcatcctc
     9001 tgtgcctgtt ttgctgcttc tgcaaagtca cacttccact ctgccttgtg catttccatg
     9061 aatgccttga gattgtcaag tacaatcctg tcagcaaaca catgttcctg atgcactttt
     9121 tcttttgcca aggattctgc ctgcttgatg tatttttcaa gtttttcaat caactggtct
     9181 gaatacacat acggaagaat tctatcttca tgtccaagcc atggagtttt ttccaacgtg
     9241 atttccagtt cttcccagaa tgctttagct ggttgtgctg caggtccgta ccagtttgtg
     9301 aaaaattcat ccatcaatgc ctttgcatcc aggtcagcgt cccacatcat tcttgcatat
     9361 aaataggtcg gaaagatgcc tgattcaatc ctgatggttc ttccttcgtt tgaaaatcca
     9421 acaactcccc attttttata cagtggcatg tcatgcatgt gtt
//
LOCUS       DAORYU010000026         9405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_5445_length_9405_cov_3.876257, whole genome shotgun sequence.
ACCESSION   DAORYU010000026 DAORYU010000000
VERSION     DAORYU010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9405
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5445_length_9405_cov_3.876257"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            344..1405
                     /locus_tag="PLW07_01550"
     CDS             344..1405
                     /locus_tag="PLW07_01550"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOL49239.1"
                     /translation="MKKIKIGVIGAGGISHAHIEAFKQLPDIEVAAISDPNETKLSFV
                     AEKYGILKRFANWEDLLKEQIDIVSICSPNAFHAQQAISALKAGKHVICEKPVAMSVE
                     EAESILETAKQTGNKFMVAFCHRFAPHSQFIKKMVNAGQMGELYYAKASCLRRRGIPG
                     LGTWFTTKKLAGGGPLIDIGVHILDLAIYLMGCPEPKLIVGVTYNKFKNQAVDGGWPP
                     VWSRVGDKPTGTFDVEDLAAGFIRFKNNTTLFLEASWAGNSEVGLKISLFGTKSGVQY
                     WTEAKPPLKIYGDINGVVTDATTEFPSVNVYHAEIEHFVNCVRNNIQPITTPEEIITV
                     IKIIQNIYKSAETGKPVEL"
     gene            1405..1932
                     /locus_tag="PLW07_01555"
     CDS             1405..1932
                     /locus_tag="PLW07_01555"
                     /inference="COORDINATES: protein motif:HMM:NF014614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOL49240.1"
                     /translation="MKRGIVIVFSGDGKGKTSAATGCAIRMLGWGKKVVYCTFFKNKS
                     GEFKILKNMKNCKLLMFCRKYPIYASASEKREFQTHFIDEWNKFLKTFHSIKKCDLIV
                     MDEILIAVRDKILTEHDIISFIDAARNRIPKINIILTGRGTTRLINKRADIVTNMCCV
                     KHPYPDIVAQKGIEY"
     gene            1995..2498
                     /locus_tag="PLW07_01560"
     CDS             1995..2498
                     /locus_tag="PLW07_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOL49241.1"
                     /translation="MQDNNEFLKIIKNRRSIRKFTQKKIPENIIKDILDCGRLAPTAR
                     NLQPWTFVVIREKQTINKLAEITDYGKFLVDASCCILVFCQDTKYYLEDGCAATENIL
                     LAAQAYGIGSCWIAGDKKPYASKIAEMLGAPGGMKLVSIIAIGYPAEIPNPPKNSLED
                     VVKWGHW"
     gene            2579..3952
                     /locus_tag="PLW07_01565"
     CDS             2579..3952
                     /locus_tag="PLW07_01565"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005960174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOL49242.1"
                     /translation="MLRTNAWKSWKDKEIEKVFQFAEDYRLFISKNKTERKFVDAVVE
                     LAKKSGFNDLSTKKEKLMPSDKIYKINKGKQIILGIIGKQPLDQGARFIVAHVDAPRL
                     DLKPSPVYEYESMAFFKTCYYGGIKKYQWVTIPLALYGIAVLKTGKMKKVEVGDNESD
                     PVFTITDLLPHLAKDQLKKTIEEGFPGENLNILAATIPSKEEEKEPVKKNIFSLLNKK
                     YGITEEDVISSEFEIVPAGPARDLGIDRSMILGYGQDDRVCAYTSFKALLENSKPEKS
                     IFCILVDQEEIGSYGTTSAQSTFFQFFLEEVAEKTECKQPLRNIYENSRALSADVSSL
                     LDPGYREAYDSKNSARIGHGVCVERTTGGRGKAGSTEPAAEYIAWIRKIFDENHVHWQ
                     PSEIGKVEQGGGGTVATFFARLNIPTIDCGTGVLSMHAPFEVTSKADIYSTYLAYKTF
                     YNAEQNA"
     gene            3945..4337
                     /locus_tag="PLW07_01570"
     CDS             3945..4337
                     /locus_tag="PLW07_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004082423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOL49243.1"
                     /translation="MHKIEIETTKRKEMIDITYMISNVLRKNSVDSGVCVVFTPHTTC
                     GITINENADPSVQTDILSTLNKLIPEQTKYTHLEGNSDAHVQSTLVGHSIFLIIEEKR
                     IQLGTWQGIFLCEFDGPRTREVWIQILK"
     gene            4779..5207
                     /locus_tag="PLW07_01575"
     CDS             4779..5207
                     /locus_tag="PLW07_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOL49244.1"
                     /translation="MEPVLVNLASKTFKNPYPAADTIISIEGGVVMVYRKNPPSGWAI
                     PGGFINCGESAEEAAVREAKEETGLDIDHLALFGVFSDTERDPRFHTISIVYTGKGRG
                     TPKSGDDASKVAIFSKNDLPGDIAFDHKKILNQYFKKRRI"
     gene            5212..6213
                     /gene="trpS"
                     /locus_tag="PLW07_01580"
     CDS             5212..6213
                     /gene="trpS"
                     /locus_tag="PLW07_01580"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOL49245.1"
                     /translation="MNLRTSYKGRVLSGMRPTGPLHIGHLFGALSNWEKLQNDGYQCF
                     YMIADWHALSTEYQNPSEIKNNILEIAIDFLSCGLDPEKSTIFIQSKVKEHTELNLIL
                     SMIVPVSWLERNPVYKEQVQELKEKDLTTYGFLGYPVLQAADILIYLAEYVPIGVDQL
                     PHLELTREIARRFNFLYGNYFPEPKEMLAQTPKVPGTDGRKMSKSYGNAIFLKDPDAE
                     ILSKVSKMFTDPKRLYKKDPGHPEECPVFAYRQLVSENFREIKQKCLGAEIGCVECKK
                     QMGQDLIKYLKPVREKRKNLAENPEKVWKILLNGSEKASSVAGKTMKDVKKLIGLEF"
     gene            6213..7349
                     /gene="hisC"
                     /locus_tag="PLW07_01585"
     CDS             6213..7349
                     /gene="hisC"
                     /locus_tag="PLW07_01585"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOL49246.1"
                     /translation="MANKIETLAKKSLAFIEPYIPGKPAEVLFRERKLAKIVKLASNE
                     NPLGPSPAAVKALKKRIYGINKYPEGSAYLLKEKLAGILKVNTNNIIVGSGSSEIISM
                     AIQSFCEPEDEIIFPHPSFIIYKILAHASGVKPVTVNLNSDFSYNLDAFFEKITPRTK
                     LIILCNPNNPTGSHIQRTQLERFLRNIPDRILLLSDEAYIEYAEDPNFGSALEWINKK
                     NVIVARTFSKIYGLAGLRIGYGISSEDIINIMEKIRPPFNTSNCAQEAATAALDDTVF
                     VKKSIENNKKQKRYLIKHLTEIGFRMFPSEANFLFCKSSQNAELICRELEKRGIIIRP
                     MSGFGIKDNFLRITIGKPSENQLLVKHLTEITRGANDEKTRSQY"
     gene            7324..8061
                     /locus_tag="PLW07_01590"
     CDS             7324..8061
                     /locus_tag="PLW07_01590"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010586973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOL49247.1"
                     /translation="MKKLGVNIDHIATLRQARKGRFPEPVFAAGICETAGCDSIVCHL
                     REDRRHIQDRDVLALKNTVRRLNLEMAMSEEIIKIALQVQPAQVTLVPEKREELTTEG
                     GLDVSSNLKKVKEATEIFHKKGITVSLFIEPDFKQIEASKEVGADAIEIHTGRYAEYF
                     EKDQDQEWNEELQRIRKASEFAVSTGLKTHAGHGLDYRNIYPILNISHIEEFNIGYSI
                     ICRAIFTGLHRAVRDMARIIKSSNRDD"
     gene            complement(8394..9119)
                     /gene="tatC"
                     /locus_tag="PLW07_01595"
     CDS             complement(8394..9119)
                     /gene="tatC"
                     /locus_tag="PLW07_01595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOL49248.1"
                     /translation="MDSQDVSFFDHLEELRRRILFILLWFFICCTISYVLHNQILGFV
                     IRPLKIFQQNPVFISPVEPFFSIIKLTLFLGLILSCPFIFFQMYIFVKPALSDEQDKV
                     FFFCLIVGLVLFYTGLSVGYYFIVPMGLKILLGFGQNLMVPLVTIGYYLSFLIWITLS
                     LGLVFQLPVILAFLGISGIIDIDQMRRMRKFVYVAAFIISAIITPTTDAITQTIIAGT
                     LIVLYEITLVFLKFFLKHKVLKI"
     gene            complement(9122..9307)
                     /locus_tag="PLW07_01600"
     CDS             complement(9122..9307)
                     /locus_tag="PLW07_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49249.1"
                     /translation="MDNKKNENTVDAKKKVIVWLAVLVVLIASSYFIYNYIGDANSVV
                     TDMFFKDKEDKENKSEH"
BASE COUNT         3493 a         1633 c         1809 g         2470 t
ORIGIN      
        1 ttacaaaagg gggactaagg gggatttttt gaaatctccc ccagcccctc ttttttaaag
       61 aggggtgata gtatgagtta attttacaaa gaggagtgat agtgcgaggt tatgttataa
      121 agaggggagg tagtatgagg tgattttgca aagaaaagtg tgattttgta ctgccccgta
      181 tttcagacca ctctattcta ccttattttt tatcttctct taatgtgcag cagtataaca
      241 atacactttc atagagatta tttttgagga aaggcgtact ctttacttca aaatttcttt
      301 tgataaaata tagaagaaaa actgaacccc cgggaggaga caaatgaaaa aaataaaaat
      361 aggtgtaata ggagctggag gaatttccca tgctcatatt gaagcattca aacaactacc
      421 tgatattgaa gttgcagcaa taagcgaccc aaatgagaca aaactttcat tcgtggcgga
      481 aaaatacgga attctaaaaa gatttgctaa ctgggaagac ctgctcaaag aacagatcga
      541 tattgtcagt atatgttccc ccaatgcatt tcacgctcaa caggcaattt cagcattgaa
      601 agcaggaaaa catgtcatct gtgaaaaacc agttgcaatg agtgttgaag aagcagaaag
      661 cattctggaa acagcaaaac aaaccggtaa caaatttatg gttgctttct gtcatagatt
      721 cgcccctcac agccagttta tcaaaaaaat ggttaatgcc ggacagatgg gagaattata
      781 ctatgcaaag gcatcatgtt tgagaagaag aggcattcca ggccttggca cctggtttac
      841 tacgaaaaaa cttgctggag gaggcccctt gattgatata ggtgtccata ttcttgatct
      901 cgcgatatat ttgatgggat gccctgaacc aaaattgatt gttggtgtga cgtataataa
      961 gtttaagaat caggcagtgg acggaggttg gccacctgtg tggagtagag taggggacaa
     1021 accaactggc acttttgatg tggaagacct tgctgcagga tttataagat ttaaaaacaa
     1081 tacgaccctg tttcttgaag caagctgggc cggtaattca gaagttggac tcaaaatatc
     1141 tctttttgga acaaaatctg gcgtacagta ctggacagag gcaaaaccac cattaaaaat
     1201 ttatggtgac attaatggtg ttgttacaga tgccacaacc gaattcccgt cagtgaacgt
     1261 ttatcatgct gaaattgaac actttgtcaa ttgcgtgaga aataacatcc agccaatcac
     1321 cacgcctgaa gaaattatta ctgttataaa aattatccag aatatctata aatccgcgga
     1381 aacaggtaaa ccagtggaac tttgatgaaa agagggattg taattgtttt ttctggagat
     1441 ggaaaaggta aaacatctgc tgctacagga tgtgcaataa gaatgttggg atggggaaaa
     1501 aaggtcgtgt actgcacttt ttttaaaaac aaatccgggg aattcaagat actgaaaaac
     1561 atgaaaaatt gtaaattgtt aatgttctgc aggaaatatc ctatttatgc gtcagcgtca
     1621 gagaaaagag aattccaaac tcattttatt gatgaatgga ataaatttct aaaaacattt
     1681 catagtataa agaaatgtga tctgattgtg atggacgaaa tccttatcgc tgtgcgggac
     1741 aaaatattaa cagaacatga tatcatttct tttattgatg cagcgagaaa tcgtattcca
     1801 aaaatcaata tcatccttac aggacggggc acaaccagat tgatcaacaa aagagcagat
     1861 attgttacaa atatgtgttg tgtaaaacat ccgtatccag atatagttgc tcaaaagggg
     1921 attgaatatt aatatgaaac agataggaaa cagacaaaat gtttttttta tgaagttcag
     1981 aggatggaaa aaacatgcag gacaacaacg agtttctaaa aataataaaa aacaggcgaa
     2041 gcatcaggaa atttactcaa aaaaagattc ctgagaatat tatcaaggac attcttgatt
     2101 gcggaagact cgcacccaca gcaagaaact tacaaccctg gacattcgtt gtgataagag
     2161 aaaaacagac aattaataaa ttggcagaaa ttactgatta cggcaaattt cttgtggatg
     2221 cttcgtgttg cattcttgtt ttctgtcagg acacaaaata ttaccttgaa gatggctgtg
     2281 cggcaacaga aaatatcctt cttgccgcac aagcgtatgg aattggcagt tgctggatcg
     2341 ctggcgataa aaaaccatat gcatcaaaaa ttgctgaaat gttaggagca cccggtggga
     2401 tgaaacttgt atcaattatt gctatcgggt atcctgctga aataccaaat ccccctaaaa
     2461 attccctgga ggatgttgtt aagtggggac actggtaaat tgactaatag tgtatttgta
     2521 attacaatac aaattacaaa ttactacgca ttatccgcca cttaatactg gaggagttat
     2581 gttaagaaca aatgcatgga aatcctggaa agacaaagaa atagaaaaag tatttcaatt
     2641 tgcagaagat tacagattgt tcatatcaaa aaacaaaact gaaaggaaat ttgttgatgc
     2701 tgttgtagaa ttggctaaga aatcgggatt taacgacctt tcaacaaaaa aagaaaaact
     2761 gatgccatcg gataaaattt ataaaatcaa taaaggcaaa cagattattt taggtatcat
     2821 tggaaaacaa cccctcgatc aaggagcaag atttattgtg gcacacgtcg atgccccaag
     2881 attggacctt aaaccaagtc ctgtatatga atacgaaagt atggcatttt ttaagacatg
     2941 ttattatggc ggaatcaaaa aatatcaatg ggtaacaata cctcttgccc tctatgggat
     3001 agcagttttg aaaacaggaa agatgaaaaa agttgaagtg ggagataatg aatctgatcc
     3061 ggtattcacc attacagatt tgttgccaca tctggcaaag gatcagttga aaaaaacaat
     3121 tgaagaagga ttcccagggg aaaatcttaa tattcttgct gccacgattc catcaaaaga
     3181 agaggaaaaa gaacctgtga aaaaaaatat tttttctctt ctaaataaaa aatatggaat
     3241 aactgaagaa gatgttataa gcagtgagtt tgaaatagtt ccggctggtc cagcaagaga
     3301 ccttggtatt gatagaagta tgattctggg atatggtcag gatgataggg tctgtgcata
     3361 cacaagtttc aaagccctgc ttgaaaacag taaacctgaa aaaagcatat tttgcatact
     3421 tgttgaccag gaagaaattg gcagttatgg aacaacatcc gcacagtcaa cattctttca
     3481 attttttctt gaagaggttg cagagaaaac agaatgcaaa caaccattga gaaatatcta
     3541 tgaaaattcc agagcactat cagcagatgt ttcttccttg cttgaccctg gttatagaga
     3601 ggcgtatgat tcaaaaaact cagccagaat aggacacgga gtgtgcgtag aaaggaccac
     3661 cggtggcaga ggaaaagcag gaagcaccga acctgctgct gaatatatcg catggataag
     3721 aaaaattttc gatgaaaatc atgtacactg gcaaccgtca gagatcggaa aggttgaaca
     3781 gggtgggggc ggaacagtag caacgttttt tgcacggctc aacatcccca cgattgattg
     3841 cggaacagga gtactatcca tgcacgcacc cttcgaagtt acaagtaaag cagacatata
     3901 ctcaacatac cttgcctaca aaacatttta taacgcagaa caaaatgcat aaaatagaga
     3961 ttgaaacgac aaaacgcaag gaaatgattg acatcacgta catgatttca aatgtgctga
     4021 ggaaaaattc cgtcgatagt ggtgtatgtg tggtatttac cccacataca acctgcggaa
     4081 tcacaatcaa cgaaaatgca gatccctcag tacaaacaga tatactatca actctaaata
     4141 aattaattcc agaacaaacc aaatacactc atctggaagg aaattccgat gctcacgtcc
     4201 aatccacatt agttggacat tctattttcc ttattattga agaaaaacga atacaactcg
     4261 gaacatggca gggtatattt ttatgtgagt ttgatggtcc ccgaacgaga gaagtatgga
     4321 tacaaatctt gaaataatta agttaataag ttatgaattt cataaatgaa attagcaata
     4381 tggaaaaaaa tcgttatttt atatctgaca ggataattga aattttgtat gacaaccacg
     4441 attccccaac tgtgaacaaa atcgtcattg atggtttatc tatctttgcc tatacatcaa
     4501 aaaataagaa aattccgaat gcaacgatat ctctgacagg attaaaaaaa gaaaaaacat
     4561 tcaagatggc attaaaaaaa ttcttccatc aacaaatgaa gcattctcat acaatctcat
     4621 ttttcatatc caatagaaaa acagggaaac ttcatcccgg tgaaatcagc cgcattatta
     4681 tcgaagaaat catcggtcaa tatttcaaag aaaccaacgt aaaagaattc aaactggtat
     4741 ttaccgaaaa aaaagatatg gataaatgcg ttgaaatcat ggaaccggta ctggtcaatc
     4801 ttgcttcgaa aacatttaaa aatccttatc ctgccgccga tacaattatc agtatagaag
     4861 gaggcgtagt aatggtgtat agaaaaaacc ctccttcagg gtgggcaatt ccaggtggat
     4921 ttatcaattg tggagaaagc gccgaagaag ccgcagttag agaagcaaaa gaggaaacag
     4981 gtcttgatat agatcatctc gcattattcg gagttttttc agacactgaa agagatccga
     5041 gattccacac aatatcaatt gtttacacag ggaagggcag gggaacccct aaatcaggcg
     5101 atgacgcatc aaaagttgcg attttctcga aaaatgattt acctggagat atcgcatttg
     5161 accacaaaaa aatcttaaac caatatttca agaaaaggag aatatgaaat tatgaactta
     5221 agaacttcat ataaaggaag ggtattaagt ggaatgagac caaccggacc gcttcatatt
     5281 ggacatttat tcggagcatt atctaactgg gaaaaacttc aaaacgatgg gtatcagtgt
     5341 ttttatatga ttgcggactg gcatgctttg agcacagagt atcaaaatcc ttcagagata
     5401 aaaaataata ttttagaaat tgccatcgat tttctatcat gcggccttga ccctgaaaaa
     5461 tcaacaatat tcatacaatc aaaagtgaag gaacataccg aactaaatct tattttatca
     5521 atgattgtgc cggtttcgtg gcttgaaaga aatcccgtct acaaagaaca ggttcaggaa
     5581 ctgaaagaaa aagacctcac cacatatgga tttcttgggt acccggttct tcaggctgct
     5641 gatattttaa tctatctcgc tgaatacgta cccattggag tggatcaatt accgcatctg
     5701 gaacttacaa gagaaatcgc acgtagattt aactttctct atggaaacta ttttcctgaa
     5761 cccaaagaaa tgcttgctca aacaccgaag gtacccggaa cagacggtag aaagatgagc
     5821 aaatcatatg gaaatgctat ttttttgaaa gaccctgacg ccgaaatttt gtcaaaggtt
     5881 tcaaagatgt tcacagaccc aaaaagattg tacaaaaaag accctggaca cccagaagag
     5941 tgtccggtat ttgcgtacag acaacttgtt tcagagaatt tcagggaaat caagcaaaag
     6001 tgcctcggag cagagatagg atgcgtggaa tgcaagaaac aaatgggaca ggatttaata
     6061 aaatatttga aaccggtaag agaaaagagg aaaaaccttg ctgaaaaccc tgaaaaagtg
     6121 tggaaaatcc tgttaaatgg atcagaaaaa gccagttcag tggcaggaaa aaccatgaaa
     6181 gacgttaaaa aacttatagg actggagttt tgatggcaaa taaaattgaa acacttgcga
     6241 agaaaagtct agcatttatt gaaccctata taccgggaaa acctgctgaa gtccttttca
     6301 gggaaagaaa attagcaaaa attgtaaaac tggcctcaaa tgaaaaccct cttggtcctt
     6361 ctcctgcagc agtcaaggca ttgaaaaaaa ggatttatgg cataaacaaa tatccagaag
     6421 gttctgcgta tctattaaaa gaaaaacttg caggtatatt gaaagtgaac acaaacaata
     6481 tcattgtggg aagcggttca agcgaaatca tcagcatggc aatccaatct ttttgtgaac
     6541 cagaagatga aataattttt ccccatccct cgttcatcat ttataaaatt cttgctcatg
     6601 cttctggagt gaaaccagta acagtgaacc ttaatagtga tttctcatat aatctggacg
     6661 ccttttttga aaaaattact cccagaacca aacttattat actctgtaat ccaaacaatc
     6721 ccacaggttc tcatatacaa agaacgcaac ttgaacgttt tttaagaaat atccctgata
     6781 gaattctttt gctatcagac gaggcatata tcgaatatgc cgaagacccg aacttcggca
     6841 gtgcacttga atggattaac aaaaagaatg ttatagtagc aagaaccttt tccaaaatat
     6901 acggccttgc cggattgaga atcggatatg gaatttcatc agaagacatc atcaatatta
     6961 tggaaaaaat caggccacca ttcaatacaa gtaattgtgc tcaggaagca gcgacggctg
     7021 cattggatga tacagtattt gtaaaaaaat ccattgaaaa taacaaaaaa cagaaacgct
     7081 atctgataaa acatttaaca gaaattgggt tcagaatgtt tccgagtgaa gcaaatttcc
     7141 tgttctgcaa atccagtcaa aatgctgaac tcatatgcag agaacttgaa aaaagaggaa
     7201 ttattattcg tccaatgtca ggatttggaa taaaagacaa tttcttacga ataacgattg
     7261 gaaaaccttc agaaaatcaa ttattagtca aacatctgac agaaataacc aggggggcaa
     7321 atgatgaaaa aactcggagt caatattgat catattgcaa cattaagaca ggcaagaaaa
     7381 ggtcgtttcc ctgaaccagt atttgctgct ggaatctgtg aaacagcagg gtgcgactca
     7441 attgtctgcc atttaagaga agatagaaga cacatccagg acagggacgt actggcatta
     7501 aaaaatacag taaggagact caatcttgaa atggcaatga gcgaagaaat aataaaaatt
     7561 gccctgcagg ttcagccagc ccaggtaact ctggtgcccg aaaaaaggga agaattaaca
     7621 accgaaggcg gccttgacgt ctcgtcgaac ttaaaaaaag ttaaagaagc aacagaaatc
     7681 ttccataaaa aaggaatcac tgttagtttg tttatagaac cagattttaa gcaaatagaa
     7741 gcatcaaaag aagtaggcgc agatgccatc gaaatacata cagggcgata cgcagagtac
     7801 tttgaaaaag accaggacca ggaatggaac gaggaattgc aaagaatccg caaagcctca
     7861 gaatttgcag tttcaactgg attgaaaacg cacgcaggcc acggcctcga ttatagaaac
     7921 atatacccaa tcctcaacat ctcgcacata gaggaattta atataggtta cagtatcata
     7981 tgccgagcca tattcacagg ccttcaccgg gcagtacgag acatggcacg aataataaaa
     8041 tccagcaaca gagacgatta aacaaaaaat aaagccactt ctgggagaaa aacgaaaaga
     8101 aacacataaa caagatcaaa ttcgcccaaa aataccttcc accaaataaa tcctatccag
     8161 gtaaaatagg acaagtagat atcagtacgg ccagagaaaa accaataaat agatataaag
     8221 atttaacatc ttgaaaatat tgaagttata aattataacg tcgcataaga tatattatgt
     8281 taacttttat gttacgtaac gtagccgaga attccttaaa tcctacgcaa actaaaggaa
     8341 atactatttg tcagtcaagt taacatttaa tataaaatta cttattataa gttttagatt
     8401 ttcaatactt tatgtttaag aaaaaattta agaaaaacga gcgtaatctc ataaagaaca
     8461 ataagggttc cagcgataat cgtttgagtt atagcatctg ttgttggagt aataattgca
     8521 gaaattataa aagcggctac atacacaaac tttctcattc ttctcatctg gtcaatatca
     8581 ataatcccgg aaatccccag aaacgccagg ataacaggca gttgaaaaac aagacccagt
     8641 gaaagagtta tccagatgag aaaagaaaga taatatccta tggttaccaa tggaaccatt
     8701 aaattttgcc cgaaacccag aagtattttc agtcccatcg gtacaataaa ataataccct
     8761 actgaaagac cagtgtaaaa taacaccaat ccaacaatta ggcaaaagaa aaacacttta
     8821 tcttgttcat cgctaagtgc tggttttaca aatatataca tttgaaaaaa aataaaagga
     8881 cagcttaaaa taaggccaag aaaaagcgta agttttatga tagaaaagaa tggctctaca
     8941 ggtgagataa acactggatt ttgttgaaat atttttaagg gtcttataac aaacccaagt
     9001 atttgattat gaagaacata agaaatagta cagcatataa aaaaccataa aaggataaaa
     9061 aggattctgc gtctgagttc ttcgaggtgg tcaaaaaagg acacatcctg agaatccatt
     9121 gtcaatgctc gcttttattt tctttatctt ctttgtcttt aaaaaacata tctgtaacaa
     9181 cggaattagc atcaccaatg taattgtaaa taaaatagga agaggctatc agaacaacta
     9241 atacagccag ccatacaatt accttctttt ttgcatccac agtattttca ttctttttgt
     9301 tatccataat atctccctct catataaatt ttaatatatt aacttttata aattataact
     9361 tattaaattt aataattttt aacttattaa tttgtacata aatat
//
LOCUS       DAORYU010000027         9384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_5453_length_9384_cov_4.072677, whole genome shotgun sequence.
ACCESSION   DAORYU010000027 DAORYU010000000
VERSION     DAORYU010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9384
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5453_length_9384_cov_4.072677"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="PLW07_01605"
     CDS             <1..304
                     /locus_tag="PLW07_01605"
                     /inference="COORDINATES: protein motif:HMM:NF014799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOL49250.1"
                     /translation="LNQEGISIGVINCSCPVEIDQDAMESGIKTGLIISYEDHVADSG
                     LGMTIAAHIAEKQVNVKFHRFGIRKYGSSGTPDELYAEHGLDPETMKSFIKNVLKK"
     gene            318..521
                     /locus_tag="PLW07_01610"
     CDS             318..521
                     /locus_tag="PLW07_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6485 family protein"
                     /protein_id="HOL49251.1"
                     /translation="MKCINRDTNLKNCTCTYISCNKRGMCCECVAYHRDNGEIPGCFF
                     PREAEKSYDRSIRNFINAWNKKI"
     gene            complement(528..1343)
                     /locus_tag="PLW07_01615"
     CDS             complement(528..1343)
                     /locus_tag="PLW07_01615"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOL49252.1"
                     /translation="MDRMLQAKKSVYIATYTFGWNEGFETLEKLAKKGIDIKILLQFP
                     QENINFKHTHIKIKQWNKKNCRLHAKFIVIDGKYVFAGSANFTENSLIWDSNNIVFFD
                     NENAGKVFAENFLLLWNGSMDCKTYKDQTVEIYFSPVYDCTQIIRNEIGKAQDNIKFA
                     MFSFTSDSIAEEICRIGLKGVKIYGIFEGSQNPVSNEYDFLKRFHFFNIKKDCFVENI
                     HDKFTVIDEKTVLTGSFNYTESARKNIETLIVIKRPDITALYSRRWKHLWLWY"
     gene            1560..2609
                     /locus_tag="PLW07_01620"
     CDS             1560..2609
                     /locus_tag="PLW07_01620"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOL49253.1"
                     /translation="MKAAIFNGPEKISVGSWESPFCAEDEILMKVNYCAICGTDVRTF
                     FHGHKKVIPPAIIGHEITGEIVEIGKNVNTNLKKGDRITPVTSIGCGHCRACEKGLYN
                     LCPETKAIGYYYSGGFAEFMIIPAPAIRQNAWVTLPENVSLLEGALIEPLSCAINAQN
                     YLNIQSGDTVVIYGGGPIGFMHAVLARASGAEKVIMVDPAFDRLKKFANQFEDIILID
                     PNTENTVARIKDITKNYGAEVIITACPAKQAQVEAFSIAAPCSRISFFGGLPKDDSMI
                     TIDSNLIHYQEISVFGAFASNRKDYQKAVDLISSRKIDPSKFITEVIPLEDIEKGIRL
                     VKNGSVLKVVVKIQE"
     gene            2613..3404
                     /gene="srlD"
                     /locus_tag="PLW07_01625"
     CDS             2613..3404
                     /gene="srlD"
                     /locus_tag="PLW07_01625"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HOL49254.1"
                     /translation="MESQVLKDKITIITGAARGLGEALSYGFAEEGAIICPWDVDESN
                     LDRVAEKLKEMNRYGRKAKIDITDENQVRNEIEAIESEFGRIDILIANAGILRAYEIT
                     DFPEEDWRKIIEVNLTGYFVCAKYVARVMKKQRSGVILQINSKSGKKGSYWNCAYAAS
                     KFGGIGLTQSIALDLAPFNIRVNAICPGNILESPLWQTSLFQQYAKKWGISIEEVKKK
                     YIDQVPLGRPCTYRDVVNVAVFLASDRANYMTGQAINVTGGQEMR"
     gene            3401..4525
                     /gene="ribD"
                     /locus_tag="PLW07_01630"
     CDS             3401..4525
                     /gene="ribD"
                     /locus_tag="PLW07_01630"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOL49255.1"
                     /translation="MKLSRDELFLKKCLSLAENGLGLVSPNPMVGAVIVKGSHIVGQG
                     WHKAYGLPHAEVEALKMAGAKAKDAILYVNLEPCCHYGKTPPCTDAIIKAGIKKVVFC
                     TLDPNPRVNGKTVVILKNHGIEVRYGLLENHAMELNESYFTYMKQKRPFITLKWAMSI
                     DGKIADASGNSKWITSEDARKFNRNLRFQYDAIMVGAGTVIKDDPELDYSVPGGVRKS
                     LLKNKRYTKIIIDGDLKCPPDTKMFLNNQGNVVVFTGKDTKPAWVKYPENVEFIEIDR
                     TPAGFLDIRQVIKFLYDKGIGRLFVEGGTNTLTSFYDCGVFDKIYVFVGGKIIGGDAV
                     YPPFKGGITPPIIDSNFRLGEIIRFSNDVLIVFKNVLRNN"
     gene            4506..5129
                     /locus_tag="PLW07_01635"
     CDS             4506..5129
                     /locus_tag="PLW07_01635"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOL49256.1"
                     /translation="MFSGIIEETGIVVEKKNKGQASEIGIRAYTVLDDLHEGDSISID
                     GVCLTVEKINRGIFYATISRETKKLTTLGYVRTGSKVNLERALKYGERVGGHFLSGHI
                     DFTGTLSDKKNEGDNYIIKVQVPAHFIKYFVRKCSVGIDGISLTVADIIGNQIIIWLI
                     PYTIEHTTIGEKNIGDKVNVETDIIVKSTVEGKTHREKYEISKEAIF"
     gene            5101..6357
                     /locus_tag="PLW07_01640"
     CDS             5101..6357
                     /locus_tag="PLW07_01640"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HOL49257.1"
                     /translation="MKSVRKQFSEISTVEKAIEDLKKGKFVVVVDDAKRENEGDLICS
                     AQKISPGHINFMAKHARGLICVALTQERIRELNLSPMVEDNTALHQTGFTVSVDAKNG
                     VTTGISVYDRAKTIKLLINPSTKPEDLARPGHVFPIQAKHGGVLVRAGHTEAAVDLAR
                     IAGHYPAGVICEIMADDGSMARMPELLKFAKKHNLKIITIADLIKYRRMREKLIERIL
                     SVELPTKFGTFDLFLYRDKIEGNNHLALVKNLPENKKIPLVRVHSQCLTGDIFHSFRC
                     DCGDQLHRSLEMISQHGGALIYLPQEGRGIGLADKLKAYKLQAEKKLDTVEANLYLGY
                     PDDLRDYGIGAQILLDLGLTNIRLLTNNPRKIIGLEGYGIQIVERVPIEVAPNPHSIK
                     YLETKRLKMKHMIKNVCSFQNKGRKK"
     gene            6354..6818
                     /gene="ribH"
                     /locus_tag="PLW07_01645"
     CDS             6354..6818
                     /gene="ribH"
                     /locus_tag="PLW07_01645"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOL49258.1"
                     /translation="MRIFEGNLSAEGKRFGIVISRFNEFLTKNLLDGAMDCLKRHGAS
                     DEGIDIVWVPGSFEIGFAARKMASLKKYNALICLGVIIRGATPHFEYVAGQVTRILGQ
                     INSEGVVPVTYGIIIADSLEQATERVGTKMGNKGWSAALSAIEMANINDLLK"
     gene            6815..7237
                     /gene="nusB"
                     /locus_tag="PLW07_01650"
     CDS             6815..7237
                     /gene="nusB"
                     /locus_tag="PLW07_01650"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOL49259.1"
                     /translation="MKKILRNARISALRACYQLDIHNLMVEEKVVDVLKDEEQNQQVY
                     HLARTLVMGVIKNLQFIDGLLAKYCTNWDISRMSYIDRNILRIAVYEMVFVPEVPDIV
                     AINEAIEISKIYSSQDAGKFINGILDSIRKELISSRTG"
     gene            7257..8201
                     /locus_tag="PLW07_01655"
     CDS             7257..8201
                     /locus_tag="PLW07_01655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOL49260.1"
                     /translation="MKRLMGVLFKISVSAFFIFLITRKIDISQMIGILRNVYMPFFIA
                     IILINYVVTFFMALRWYFVLDEFRKKISFASVWNLSLIGLYFNIILPTGAGGDAIKIF
                     YITRDQEEKLKLGTSVIFDRFIGSSSTIFMAILSLLYYQRHLPVKLKISLVFLFALVL
                     FIWFMIFWDKLAIFTGKVFPHKIRQKLKAFYNHLRDYGINRRVFINAFLASIGVQVLS
                     VYVQYLCMIMISPAGTSPVPFPLFFIFIPIIWLSAMVPSLGGLGIREYGYLFFFKPYI
                     GQEAAIAMALINLVLIFFQALLGGVIFLFLRPGRQDRK"
     gene            complement(8116..8757)
                     /locus_tag="PLW07_01660"
     CDS             complement(8116..8757)
                     /locus_tag="PLW07_01660"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOL49261.1"
                     /translation="MFSIDFTHAKWILISGSIPPGIPENFYAKLINKIRRRGKNTFIG
                     LDTDGKFLEYGIREKPDLIKPNIWELERLMAKKILNFEHLLKAGQKLLNSGIPFIIVT
                     MGNKGAAGFSKNGFFYAKGPIINNPGSVGCGDALLAGFVLSFSKTGNFKQSLRFAVAS
                     GTAKAARPFTDFPCPGEVKKILKKTSICGLDDLDEETKKSLLPEVPEKKSKPD"
     gene            complement(8790..9134)
                     /locus_tag="PLW07_01665"
     CDS             complement(8790..9134)
                     /locus_tag="PLW07_01665"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOL49262.1"
                     /translation="MQQNSFITIGLNPCLDVNCYVKEFKSDDVIRINKKVVSAGGKAI
                     NIAKFLCQFDANIQTIGMVGGYNGIRFKNLLKNSGISTDLMFDIDGETRENYNFFLKM
                     VQFYDSTNPVLP"
     gene            complement(9143..9316)
                     /gene="rpsU"
                     /locus_tag="PLW07_01670"
     CDS             complement(9143..9316)
                     /gene="rpsU"
                     /locus_tag="PLW07_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOL49263.1"
                     /translation="MSVEKQHHEHFEKLFKQFKREVEKEGIIREIKKREFYEKPSQLR
                     RRKKIRKYRTQSV"
BASE COUNT         2950 a         1493 c         2021 g         2920 t
ORIGIN      
        1 gctcaatcag gagggaatca gtattggtgt tattaattgt tcctgtcctg ttgaaatcga
       61 tcaggatgca atggagtccg ggataaaaac tggtttaatc atttcttatg aggaccatgt
      121 ggctgattca ggacttggta tgaccatagc ggcacatatt gcagaaaagc aggttaatgt
      181 aaaatttcac aggtttggta tcagaaaata tgggagttcc ggtacccctg atgaattgta
      241 tgccgaacac gggcttgatc ctgaaacaat gaaaagtttt ataaaaaacg ttcttaaaaa
      301 gtaagcagga ggcaaccatg aaatgtatta atagggatac gaatctgaaa aattgtacct
      361 gcacatatat ttcttgcaac aaacgtggta tgtgttgtga atgtgttgcg tatcacagag
      421 ataatggcga gattcctggt tgttttttcc cgcgtgaggc agaaaaaagt tatgataggt
      481 caataagaaa ttttattaat gcctggaaca aaaaaatctg attattgtca ataccataac
      541 cacaggtgtt tccatcttct gctgtaaagt gctgtgatgt caggtctttt tatgacaatg
      601 agtgtttcaa tattttttct tgctgattct gtgtaattga aagaacctgt taacacagtt
      661 ttttcatcaa tgactgtgaa cttatcatga atgttttcca caaagcaatc ttttttgatg
      721 ttaaagaaat ggaatctctt caaaaaatca tattcatttg atactggatt ctgagaaccc
      781 tcaaaaattc cataaatttt gacgccttta agtccgatcc tgcaaatttc ttctgcaata
      841 gaatcagagg taaatgaaaa cattgcaaat ttgatattat cttgggcttt ccctatttca
      901 tttcttatga tttgtgtgca atcataaaca ggtgaaaaat atatttcaac tgtctgatcc
      961 ttataggtct tgcaatccat tgaaccattc cacagaagca aaaaattttc tgcaaacacc
     1021 ttacctgcat tttcattatc aaaaaataca atattattcg agtcccatat aagagaattt
     1081 tcagtgaaat tggcagaacc tgcaaataca tatttcccat caatgacaat gaattttgca
     1141 tgcaaacgac aatttttttt attccattgt ttgattttta tatgggtatg tttgaaatta
     1201 atattttcct gtggaaattg cagaagtatt ttgatatcaa tgcctttttt tgcaagtttt
     1261 tcaagggttt caaatccttc attccatcca aatgtataag ttgcaatata aactgatttt
     1321 tttgcctgaa gcattctgtc catgattaat ctgtcaacct tttgggaagg agaaaaatac
     1381 accctgatat ctgaacaata cagaatggtt tgtaatagca acaataaaat ggcaaattga
     1441 ttgaattttc tcatgaaaaa attttaatgt ctgtttgaga ttttttcaac ctgtaaattt
     1501 ccataaatga taaaagtgaa gtaaaataat taataaaatt gttcaaaaag gagggaattt
     1561 tgaaagccgc aattttcaat ggtccagaaa aaatcagtgt agggagctgg gaatcgccat
     1621 tttgtgcaga agatgaaatt ttgatgaagg tcaattattg tgcgatatgc gggaccgatg
     1681 taagaacttt tttccacgga cataaaaagg ttattccacc agccatcatc gggcatgaaa
     1741 tcactggaga aattgttgaa atcggaaaaa atgtgaacac gaacttgaaa aaaggtgaca
     1801 gaattacacc ggtgacttcc ataggttgtg gacactgcag agcgtgtgaa aaaggtcttt
     1861 ataatttgtg ccctgaaaca aaagcgatcg gttattatta ttcaggagga tttgctgaat
     1921 ttatgattat tccagcgcct gctattagac agaacgcctg ggtgacttta cctgaaaatg
     1981 tttctttact ggaaggggct cttatagaac ctctttcttg tgcgatcaat gctcagaatt
     2041 acttaaacat acagagtgga gatactgttg ttatctatgg aggtggtcca attggattta
     2101 tgcatgctgt tcttgccaga gcatcaggag cagaaaaggt aatcatggtt gatcctgctt
     2161 ttgatcgttt aaaaaaattt gcaaaccagt ttgaagatat tattttgata gacccaaata
     2221 ctgaaaatac tgttgccaga ataaaagata ttacaaaaaa ttatggagct gaagtaatta
     2281 ttaccgcctg tcctgcaaag caggcccagg ttgaggcatt ttccattgcg gcaccgtgca
     2341 gcagaatcag tttcttcggg gggcttccaa aagatgattc aatgattacg attgacagca
     2401 atctcattca ttaccaggag atttcagttt ttggtgcctt tgcttcaaat agaaaagatt
     2461 accagaaggc agtggattta atatcttcga ggaaaataga tccatcaaaa tttattactg
     2521 aagttatacc actggaagat attgaaaaag gaatccgtct tgtgaaaaat ggtagtgtat
     2581 taaaggttgt cgtgaagata caggagtgaa tcatggaatc acaggttctg aaagataaaa
     2641 tcacaattat cacaggagca gcaagaggtc ttggagaagc gttgagttat ggatttgcag
     2701 aagagggagc aattatatgt ccctgggatg tggacgaaag taatcttgat agagttgcgg
     2761 aaaaactgaa agaaatgaac agatatggca gaaaagcaaa gattgatatt actgatgaga
     2821 atcaggtgag aaatgaaatt gaggcaattg aatcagagtt tggcaggatt gatattctta
     2881 ttgcaaacgc gggtatcttg agggcttatg aaattacaga ttttccagag gaggactgga
     2941 ggaaaattat tgaggtgaat ttgacaggat attttgtctg cgcaaaatat gtagcaaggg
     3001 tgatgaagaa acagcgaagc ggagtcatcc ttcagattaa ctcaaagtcg ggtaaaaagg
     3061 gaagttactg gaattgtgct tatgcagcaa gcaaattcgg tggaattggt ctgacacaaa
     3121 gtattgcact tgaccttgcg ccatttaata taagggttaa tgcaatatgt ccgggtaata
     3181 ttcttgagag tcctctgtgg cagacaagtt tgtttcagca atacgcgaaa aaatggggaa
     3241 tttcaataga ggaggttaag aaaaaatata ttgatcaggt accacttggt aggccatgta
     3301 catacagaga tgttgttaat gtggctgttt ttcttgcaag tgaccgtgct aattatatga
     3361 caggtcaggc gattaatgtc accggtggtc aggaaatgag atgaaattat ccagggatga
     3421 acttttcctg aaaaaatgtc tttctcttgc agaaaatgga cttggactgg tcagccccaa
     3481 tcctatggtc ggtgctgtta tcgtcaaagg aagccacatt gttggacagg gctggcacaa
     3541 agcttatgga ttgcctcacg cagaagtgga agcgttgaaa atggcaggcg caaaggcaaa
     3601 agatgctatt ctttatgtaa atcttgaacc atgctgccat tatggcaaaa ctcctccctg
     3661 tactgacgca attatcaaag caggtattaa aaaggttgtg ttttgtaccc tggatccaaa
     3721 ccctcgcgtg aacgggaaaa ctgtcgtaat cttaaaaaat catggtatag aagtgagata
     3781 tggcctgtta gaaaatcatg ccatggagtt aaatgaatct tattttacat atatgaaaca
     3841 gaaaaggcct tttattactc tcaaatgggc gatgagtatt gatggcaaaa ttgctgatgc
     3901 ttccggcaac tcaaaatgga tcacatctga ggatgcaaga aaattcaaca ggaacttgag
     3961 atttcaatat gatgcaataa tggtcggagc aggaactgtt atcaaagacg atccagaact
     4021 tgattattct gttcctgggg gagtcaggaa atcgcttctg aaaaataaac gttatacaaa
     4081 aattattatc gatggtgatc ttaaatgtcc accagatacg aaaatgtttt taaataacca
     4141 gggaaacgtg gttgttttta caggtaaaga tacaaaacca gcatgggtaa aatacccgga
     4201 aaatgttgaa tttattgaga ttgataggac tcctgcggga tttcttgata ttcgacaggt
     4261 gatcaaattt ttgtatgata agggcatagg caggttgttt gttgaaggag gtacaaacac
     4321 tttgacgtct ttttacgact gtggagtgtt tgataagatt tatgtatttg ttggcggcaa
     4381 aattattggt ggtgatgcgg tgtatccgcc atttaaaggt ggtattacgc cgccgataat
     4441 agattcaaat tttcgtcttg gagaaattat caggttttca aacgatgtgt tgattgtgtt
     4501 taaaaatgtt ctcaggaata attgaagaaa caggaatcgt tgttgaaaag aagaataaag
     4561 ggcaggcatc agaaattggc ataagagcat acacagtcct tgacgatttg catgaagggg
     4621 acagtatcag tatagatggg gtatgtttaa cagttgagaa aatcaatcgt ggtatatttt
     4681 atgcaacaat ttcccgcgaa acaaagaaat tgacaaccct tggttatgtt agaacaggat
     4741 caaaagtcaa tcttgaacgg gcattgaaat atggtgaaag agtgggtggt cattttcttt
     4801 caggacatat tgatttcact ggtacacttt cagacaagaa aaatgaggga gataactata
     4861 ttattaaagt gcaggtccct gctcatttta taaagtactt tgttcgcaaa tgttctgttg
     4921 ggatagacgg aatttctctt actgtggctg atattattgg caatcagatt attatatggt
     4981 taattccata tacgattgag cataccacaa ttggcgagaa aaatatcggt gataaagtga
     5041 atgttgaaac ggatatcatt gtgaaatcta cagtagaagg aaaaacacac agggaaaaat
     5101 atgaaatcag taaggaagca attttctgaa atcagcactg tggaaaaagc gatagaggat
     5161 ttgaaaaaag ggaaatttgt cgttgttgtt gatgatgcaa aaagagaaaa tgaaggagat
     5221 ttgatttgtt cggctcagaa gataagtcca gggcacatca actttatggc taaacatgcc
     5281 aggggattaa tatgtgtagc attaacccag gaaagaataa gagagttaaa tttatctccg
     5341 atggtggaag ataacacagc acttcatcaa accggattta ctgtttctgt tgatgcaaaa
     5401 aatggagtga caacaggtat ttcagtctat gatagagcaa agacgataaa acttttgatc
     5461 aatccttcca caaaaccaga agatcttgca agaccggggc atgtttttcc cattcaggca
     5521 aaacatggcg gtgtgcttgt tcgtgccggg cacactgaag cagcggttga ccttgcaaga
     5581 attgcgggac actatcctgc gggagtgata tgtgaaataa tggcggacga tggttctatg
     5641 gcgcgaatgc ctgaattatt gaaatttgca aaaaaacata acctgaaaat cattacaata
     5701 gcagatttaa taaaatacag aaggatgagg gaaaaactga ttgaaagaat attgtctgtt
     5761 gagttgccga caaaatttgg aactttcgac ctttttcttt acagggataa aatagaaggt
     5821 aacaatcatc tggctttagt aaaaaacctg ccagagaata aaaaaatacc actggtaagg
     5881 gttcattcac agtgcctcac tggagacatc tttcattcct ttagatgcga ttgtggcgat
     5941 caattgcaca ggtctcttga aatgatttca caacacggag gtgcactgat atatttacct
     6001 caggaaggca ggggcatagg tcttgcggat aaactgaagg catataaact tcaggcagag
     6061 aagaaacttg atactgttga agcaaatctg tatcttggtt acccggatga cctgcgggat
     6121 tatggtatcg gcgcccagat tcttcttgat ttagggctga ccaatattcg acttttaacc
     6181 aacaatccca gaaaaattat tggtcttgaa ggttatggga tacagattgt tgaacgcgtt
     6241 cccatagagg tggcaccgaa tccacattcc attaaatatc tcgaaacaaa aagattaaaa
     6301 atgaaacata tgattaaaaa tgtgtgttca tttcagaaca aagggaggaa aaaatgagaa
     6361 tttttgaagg aaatctttct gcggaaggaa agcgtttcgg aatcgtgata agcaggttta
     6421 atgaatttct gacgaaaaat cttcttgatg gggcaatgga ttgtcttaaa agacatggtg
     6481 cgtcagatga aggtattgat attgtctggg taccaggttc atttgagatt ggttttgcag
     6541 caagaaaaat ggcgtctcta aaaaaataca atgctctgat atgcctggga gttattattc
     6601 gtggggcaac accgcatttt gaatatgtgg caggacaggt tacaaggatt ttaggacaga
     6661 ttaacagtga aggggttgtg cctgttacct atgggattat tatcgcagac agcctcgaac
     6721 aggcaacaga aagagtcggg acaaaaatgg gaaacaaggg ctggagtgct gcgctttcag
     6781 ccattgaaat ggcaaatatt aatgatttgt tgaaatgaaa aagatcttac gaaatgcaag
     6841 aatttctgcg ttaagggcgt gttatcaact tgacatacac aacttaatgg ttgaagaaaa
     6901 ggttgttgat gtgctgaaag atgaagagca aaaccagcag gtttatcatc ttgccaggac
     6961 tcttgtaatg ggtgtgataa agaatctaca atttattgac ggattgcttg caaaatattg
     7021 tacaaactgg gatatatcaa ggatgagtta tatcgaccgt aatattttaa gaattgctgt
     7081 gtatgaaatg gtttttgttc ctgaagtgcc tgacattgtg gcaataaatg aggcgattga
     7141 aatttccaaa atttatagtt ctcaggacgc cgggaagttt atcaatggaa ttcttgatag
     7201 tataagaaag gaacttattt ccagcagaac aggatagaat ttttattgga ataaaaatga
     7261 aacgattgat gggtgttttg tttaaaatat ctgtgagtgc gttttttata ttcttgatta
     7321 ccaggaagat tgacatttca cagatgattg gaattttgcg aaatgtatat atgccatttt
     7381 tcatcgcgat cattttgatt aattatgtgg tgactttttt tatggctctt agatggtatt
     7441 ttgttcttga tgaattcaga aaaaaaatat cctttgcaag tgtgtggaac ctttctctta
     7501 tcgggttgta ttttaatatt attttgccga ctggcgcagg tggagacgca atcaaaattt
     7561 tttatatcac cagggatcag gaagagaaat tgaaacttgg tacttctgta atttttgacc
     7621 gctttattgg ttcttcgtca accattttca tggcaatact cagtttatta tattaccaga
     7681 gacatctgcc tgtgaagttg aaaatttccc ttgtgtttct tttcgctctt gtgctattta
     7741 tctggtttat gatattctgg gataaacttg ctattttcac aggcaaagtc tttccgcata
     7801 aaatcagaca gaagttgaag gcattttaca atcatctcag ggattatggt atcaaccgca
     7861 gggtttttat caacgccttt ttggcaagca tcggtgtaca ggtactttct gtttatgtcc
     7921 agtatctctg catgataatg atttctcctg ctgggacttc acctgttcct tttccattgt
     7981 tttttatttt tattccaatc atatggcttt cagcaatggt tccttcgctt ggcggacttg
     8041 gaataaggga atacgggtat ctgtttttct ttaaacctta tatcggacag gaagcggcaa
     8101 ttgcgatggc actgattaat ctggttttga ttttttttca ggcacttctg ggaggagtga
     8161 tttttttgtt tcttcgtcca ggtcgtcaag accgcaaata gatgtttttt taaggatttt
     8221 ttttacttca ccagggcagg ggaaatctgt aaatggtctg gctgcctttg ctgttcctga
     8281 tgcaaccgcg aatctcaatg attgtttaaa gttgcctgtt ttagaaaagg acagaacaaa
     8341 gccagcaaga agagcatcgc cgcacccaac ggaaccggga ttattgataa ttggtccttt
     8401 tgcatagaaa aagccgtttt ttgaaaatcc agcagcccct ttatttccca ttgttacgat
     8461 aataaaagga atacctgagt tcaggagttt ttgacctgct ttaagcaaat gttcaaaatt
     8521 caagattttt tttgccataa gcctttcaag ttcccagata tttggtttta tcagatcggg
     8581 tttttcacga atgccatatt cgagaaattt tccatctgta tcaagtccga tgaacgtatt
     8641 ttttcctctg cggcggattt tatttatcaa ttttgcataa aaattttcgg gtataccggg
     8701 tggtatgcta ccagaaataa gaatccattt tgcatgagta aagtcaatac tgaacacaca
     8761 atcaagtatt tttctcaggg tttgtttttt tatggcagga ccggattcgt tgaatcgtaa
     8821 aactgtacca tcttcaaaaa aaaattgtaa ttttctctgg tttctccatc tatatcaaac
     8881 atgagatcag ttgaaatgcc tgaatttttt agcaggtttt tgaaacggat tccgttataa
     8941 ccgcccacca tgccgattgt ttggatattt gcgtcaaact ggcaaagaaa ttttgcgata
     9001 ttaatggcct taccacccgc agatacaacc tttttgttaa ttcggatcac atcatctgat
     9061 ttgaattcct taacataaca gttaacatca aggcatggat tcaaaccaat tgtgataaaa
     9121 ctattttgtt gcattgaatt tttcacacag attgagtccg atattttctg atttttttac
     9181 gacgcctcaa ctggctgggt ttttcataga attcacgttt cttgatttcc cttatgatgc
     9241 cttccttttc aacctctcgt ttgaattgtt tgaaaagttt ttcaaagtgt tcgtggtgtt
     9301 gtttttccac agagatactg tcaccccctc cctaatatct gtctatttca aacagacggg
     9361 caccgcaatg cctgcatttt tttt
//
LOCUS       DAORYU010000028         9333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_5480_length_9333_cov_4.439427, whole genome shotgun sequence.
ACCESSION   DAORYU010000028 DAORYU010000000
VERSION     DAORYU010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9333
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5480_length_9333_cov_4.439427"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /locus_tag="PLW07_01675"
     CDS             complement(<1..1097)
                     /locus_tag="PLW07_01675"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49264.1"
                     /translation="MIPFVFFFKGFASLCAQSLLLREFYIVLHGNELSFGIMLSIWVI
                     AGAAGSFFAAKNPVRNPTLTFYLLTVFEGFWLFLSLTLIRLCRTVFNLFPGEMLNITQ
                     LIVLTLITAGIFNFSQGMRFVIGGSVFRKNTADEKITGLMYGFEAAGALTGGFFFAFL
                     LQRHTNPFQTSAIIILLSMLSCLPLFSSFIKKTAKIFMTIPYFIILFLFVYPGDFYST
                     LNTITLMKQWNIKNPPVFYKNTHYGNITVIENNKELSFMMDGNLLFTTPYPDIEWIES
                     TAHFPLLYAKKTTQILVIGAGMKGIIPEILKYHPETIDCVEINPELVNVIKHFSSSYF
                     PGYKSGKVNVTQDDAVNVIRKTKKQWDVIIL"
     gene            complement(1138..1956)
                     /locus_tag="PLW07_01680"
     CDS             complement(1138..1956)
                     /locus_tag="PLW07_01680"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOL49265.1"
                     /translation="MKKFIMLLLVCLVSICAGEQEKSKVIAKIGGKTLTLKEFQENLD
                     QMKSRFASLNYDMKKRILDQFVREKLFTTAAKDAGINLTPEQEKYIERAKTMYLINNY
                     VSNILRENPVTETDIKQTYQKNPQAYETPERRKLRHIIVQDQQQAKQILNQLKEGADF
                     GELASQNNIDATKQRGGDLGWAQKGIFVKEFENVAFSLKKGETSDIVKTQFGYHIIRV
                     DDIEEPKQKTFNEVHQEIKRKLEEQKILQIEEQLKKKYKVQVDYSVLEDAEKNK"
     gene            2060..2131
                     /locus_tag="PLW07_01685"
     tRNA            2060..2131
                     /locus_tag="PLW07_01685"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2092..2094,aa:Cys,seq:gca)
     gene            complement(2136..2786)
                     /locus_tag="PLW07_01690"
     CDS             complement(2136..2786)
                     /locus_tag="PLW07_01690"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOL49266.1"
                     /translation="MKKHLFIFDLDGTLMDAYQAIWETLLYTLSSLGYPPVDFETAKR
                     AVGHGDKHYIPVFFKPEDVEKARKIYREYHLKSLNGKVRLMPGAKQLLEKLKNSNRFI
                     AVASNRPSESGMLIIKNLGIESFFNYTVFGDQVANQKPDPEMLIKVLEFFGLEKAKAV
                     FIGDMDIDAVTGKKAGIDTLIVSTGSSTIDQIMVSNPSKICNNLFEIIDMLDKGLF"
     gene            2911..3294
                     /locus_tag="PLW07_01695"
     CDS             2911..3294
                     /locus_tag="PLW07_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49267.1"
                     /translation="MKKVVVILLAVTFAGTLVYGRDMPVIRMNEPGQAPGDNPALAGK
                     VKELLEIQKKMFDIERTVIQQDQELKQLAEQIKVLQTQLRNRIEEKLKDNMDYQTLKQ
                     RREQIRLEYQRTDFKKIDNFKKTQP"
     gene            3346..4212
                     /locus_tag="PLW07_01700"
     CDS             3346..4212
                     /locus_tag="PLW07_01700"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOL49268.1"
                     /translation="MFGGLDLIVIMFFLGGILGGAGRGFLGTVIDICGIIFGIFIGSF
                     VYAAPVKLFAKFGITGTPVELIFFILSSIVFALTFILLLEALRKKVEIKPAIDRIFGG
                     IFGSINGFVAAASILVVMSSSVRMGQEIDQAKFAPRVRDGLLKFYEKIERHNFTLPKI
                     IILPVAYKDEFGNNVKTAKFVKMNFTKFEGYTCINCGGKVKFAGYFTKYGVGLVPKLV
                     CEKCGRTSDGCQTYEGYHKLYNACVVELARQGMKFDCGTWPNHTWVTPTGPCPVDNNS
                     LELFLWREPIKY"
     gene            4218..4973
                     /locus_tag="PLW07_01705"
     CDS             4218..4973
                     /locus_tag="PLW07_01705"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOL49269.1"
                     /translation="MTPVHTIESLLTGLIAGFICGLTGMGGGTIYVPVFYFLFGFSIK
                     KAIGTSLLVIFISSVSAFFAHARGRQIDFKMSIYLVVSGIIGAQIGSAITARLPDIAV
                     KIFFICLTMFLSFNMWRDRKNEVESCPANMNFSPVKLTLIGLTGGVISGMGGVGGAIV
                     LVPLLHLCCGVPMNICIGTTLSVVIFNSLSGVIGYFYHNLVDVQTGTVTGIVAVFTAV
                     AGAKLSMKTPTRKLRKIFSVILLMAGLAVVFGR"
     gene            5012..5632
                     /locus_tag="PLW07_01710"
     CDS             5012..5632
                     /locus_tag="PLW07_01710"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOL49270.1"
                     /translation="MEQKSKALAVLKENELIERAKNKDHAAFEELVKRNQARIYNLGL
                     KLLGNKEDAADLLQETFINAYYALPKFEGKSAFSTWLYRIATNNAFMKMRKKKIPLVR
                     FESDREDSEQRYFTDVPDWSNDPRAHLKNEELKVLLEKSVDSLPGKYKTIFVLHDIEG
                     FSIEEISKMLDLSVPAVKSRIHRSRMFLRDKLSQYFQLHAGQGIRV"
     gene            5629..5877
                     /locus_tag="PLW07_01715"
     CDS             5629..5877
                     /locus_tag="PLW07_01715"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HOL49271.1"
                     /translation="MKCKKYCELISLYLDRELDQMLIDELEEHLSLCRNCLAFFHTME
                     KTVSLSRTYYKYRCCKVPRNVSSQIFYRLRIIYKEEKF"
     gene            5890..6606
                     /locus_tag="PLW07_01720"
     CDS             5890..6606
                     /locus_tag="PLW07_01720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HOL49272.1"
                     /translation="MKYSIKGWVKNSFCDWDSKVSSVIFLPQCNMRCPFCQNQELVER
                     YDDLEDIDWQTIKNYLKENRDFLDGIVITGGEPFVSDFLFDLVKEVKTMGLGIKIDTN
                     GTFPEQLNTLIDTGMIDCVAMDVKNALNTDAYSKACGINITDGMLEKIVQSTKVLMNS
                     GIDYEFRTTIVRSLHSSEDIRGIAKYLNGAKKYVLQQYRPMGVKKGFDGGELFSKEEL
                     DNLCKDISQFFQQCFVRFYG"
     gene            6599..7297
                     /locus_tag="PLW07_01725"
     CDS             6599..7297
                     /locus_tag="PLW07_01725"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HOL49273.1"
                     /translation="MAELIGNINVSWYAWVGVSFLAGIISFFSPCVLPLIPAYFSFIL
                     GSSVYEPEKSKGHLIRSVILMCCGFTIVFVTLGASATGIGRFLASHMSFFKLLAGIIM
                     LVFALETLELTHIFSFHKGFSPEFRKKPVGLNAIIFGVVLGISWTPCVGPILGAILTM
                     AATQETVAKGILLLFVYSLGLSVPLIIFAVFFSQMKKFQKFMIVHARKIRIVAGSVLL
                     LFAFYLLSRGRIWM"
     gene            7288..8148
                     /locus_tag="PLW07_01730"
     CDS             7288..8148
                     /locus_tag="PLW07_01730"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOL49274.1"
                     /translation="MDVIVIGAGPAGISAGIYAARANMSFMVIEKMYPGGKLVWIEKI
                     ENYPGFPDGIPGPDLAERFYRQATALKVNFVFQNVDRIEKVQEHFRVFSGNEYYEARY
                     IIVASGSVPKKLGIKGEDTFVGRGVSYCAICDGAFFRNKRVAVIGEGKHVITDINYLK
                     GLASVTWIRKPTGQSQNVEGVNIINGVPLEIAGEGVVKGIVVQTEKGITTVETDGVFI
                     FAGFKPSFEFLPPEVTLDQSGYIKTDSTLQSSMNGIYVCGDIRSDSLKQIVSAVSDGA
                     VAINEIRKKL"
     gene            complement(8150..8428)
                     /locus_tag="PLW07_01735"
     CDS             complement(8150..8428)
                     /locus_tag="PLW07_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49275.1"
                     /translation="MKKIKLLIWAGVILILSGWVAFIGEYAYSKKKMAELQTRIEQLD
                     SQIQAIGGISKNLDQTIAEVDRMIAEFEKLKTNLQQTRDKIEKVSKTD"
     gene            complement(8425..8709)
                     /gene="porD"
                     /locus_tag="PLW07_01740"
     CDS             complement(8425..8709)
                     /gene="porD"
                     /locus_tag="PLW07_01740"
                     /EC_number="1.2.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate synthase subunit PorD"
                     /protein_id="HOL49276.1"
                     /translation="MEEKKLSWKQLPEGDVLEAGTSKKFKTGNWRSVRPVHIPEKCIH
                     CLICWVYCPDSAIKTKDGKFFEFDYDYCKGCGICAHECPKDAISMEPEKK"
     gene            complement(8722..9303)
                     /locus_tag="PLW07_01745"
     CDS             complement(8722..9303)
                     /locus_tag="PLW07_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014116376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOL49277.1"
                     /translation="MKEMFEIRWHGRGGQGAKTAALLFGDAACSTGKYIQAFPEYGPE
                     RMGAPVVSFNRISSSPIRTHGPILNPDVVVVLDVSLVDQVDVISGLEPEKGIILLNSS
                     KEPSEFAESLKFKGRICTVDASRISIETIGREIPNTPMMGALIKVTGMLDIKEVIEDI
                     KHKLEAKFRNRPEIIEGNIKAIEMAYNQVKISN"
BASE COUNT         2751 a         1667 c         1864 g         3051 t
ORIGIN      
        1 tcaagaataa tgacatccca ttgttttttt gtttttctga tgacgttgac agcatcgtcc
       61 tgcgtaacat taacttttcc acttttgtat ccaggaaaat acgatgaaga aaaatgtttg
      121 atgacattga ccagttccgg gttgatttca acgcaatcaa ttgtttctgg atgatatttg
      181 agaatttcag gaataattcc tttcattccg gctccaataa caaggatctg tgttgttttt
      241 tttgcataaa gtaaagggaa gtgagcagtg ctttcaatcc attcaatatc cgggtaaggc
      301 gttgtgaaca ataagtttcc atccatcata aaggacagct ctttattgtt ttcaatgaca
      361 gtaatgtttc cataatgtgt atttttataa aatacaggag gatttttgat attccactgt
      421 ttcatcaaag ttatggtatt caatgttgaa taaaaatcac caggatatac aaaaagaaaa
      481 agtatgatga agtaaggaat agtcataaag attttagcag ttttcttaat aaaagatgaa
      541 aagaggggga gacatgaaag catgcttaaa agtataatga tggcggatgt ttgaaatgga
      601 tttgtatgtc tttggagtag aaacgcgaaa aaaaatccac ctgttaaggc accggctgct
      661 tcaaaaccgt acatcaagcc agtaattttt tcatcagcag tgtttttacg aaatacactt
      721 ccaccaatga caaatctcat tccctgggaa aaattaaata tgccagcagt aatcaatgtt
      781 aaaacaatga gctgtgtaat attaagcatt tctcccggga aaaggttgaa tacagttctg
      841 cataatctta ttaaagtcaa acttaaaaat aaccagaatc cttcaaaaac ggtaagaaga
      901 taaaaagtaa gggtgggatt tcttacagga ttttttgctg cgaaaaaact gcctgctgcc
      961 ccagcaatca cccatattga gagcataatt ccaaaggaca actcatttcc atgaagaaca
     1021 atgtagaact ctctcagaag taatgactga gcacataaac ttgcaaaacc cttgaaaaag
     1081 aatacaaaag gaatcattta gatgtgatgc tgacaatttt agaaaaaaac agtatatcta
     1141 tttatttttt tcagcatctt ccagtactga gtaatcaacc tgaaccttat atttcttttt
     1201 caactgttct tcaatctgaa gtattttttg ttcttcaagt tttcttttga tttcctgatg
     1261 aacttcatta aatgtttttt gttttggttc ttcaatatca tcaaccctga taatgtgata
     1321 tccaaactgg gtttttacaa tgtcgctggt ttctcccttt ttcaacgaga aagctacatt
     1381 ttcaaattct ttcacaaata tgcctttttg tgcccagccg aggtctccgc ccctttgttt
     1441 tgttgcatct atattgttct gacttgcaag ttctccgaaa tcagcgcctt ccttcaattg
     1501 attcagaatt tgttttgcct gctgttggtc ctgaacaata atatgcctca gttttcttcg
     1561 ttcaggcgtt tcgtatgcct ggggattttt ttgatatgtt tgttttatgt ctgtttctgt
     1621 aactggattt tcacgcaata tgttggaaac gtaattgtta atcagataca ttgtttttgc
     1681 tctctcaatg tatttttcct gttcaggggt gagattaatt cctgcatctt ttgcagcagt
     1741 cgtaaaaagt ttttctctca cgaactggtc aagaatcctt tttttcatgt cataattcaa
     1801 actggcaaat ctgcttttca tctggtcaag attctcctga aattccttca aagtgagagt
     1861 ttttcctcca atttttgcaa taaccttact tttttcttgc tctcccgcac aaatacttac
     1921 aaggcataca agcaagagca tgataaattt tttcatgcgt tcctcctgtt ggtttttgta
     1981 gtatcaattg tatgtattcc tgtagttaat gtcaattttt tattgacctg ctggtgaaaa
     2041 atgagtataa ttctaagacg gccaggtagc caagcggcaa ggcgacggtc tgcaaaaccg
     2101 ttattcaccg gttcaaatcc ggtcctggcc tcttttcaaa ataatccctt atcaagcatg
     2161 tcaatgattt caaaaagatt attgcatatt tttgaaggat tacttaccat aatctggtca
     2221 atcgttgaag aacctgtact tacgatcaat gtatcaatgc ccgccttttt acctgtgaca
     2281 gcatcaatgt ccatgtctcc gataaatact gcttttgctt tctcaagacc gaaaaattcc
     2341 aatactttaa taagcatttc aggatctggt ttctgattgg caacctggtc tccgaacact
     2401 gtgtaattga aaaaactttc tattccaaga tttttgatga tcaacatacc tgattcagag
     2461 ggtctatttg aagccacagc aataaacctg ttagagtttt tcagtttttc aagaagttgt
     2521 tttgctcctg gcatcagacg cacctttcca ttcaaagatt tcagatgata ttctctgtaa
     2581 atttttcttg ctttttccac atcctctggt ttgaaaaaga caggtatgta atgcttatca
     2641 ccatgtccga cagctctctt tgctgtttca aaatccacag gcggataccc aagcgatgag
     2701 agagtataca ataatgtctc ccatattgct tgataagcgt ccattaatgt gccgtcaagg
     2761 tcaaaaataa aaagatgttt cttcatgatg gattgaatta tactggattt tttgagaaaa
     2821 tcaagcccat ggataaacca tttcttgtta ttaccatgtc taaaaattga caatacggtg
     2881 gtatggttat aaattattat tggaggaaaa atgaaaaaag tagtcgttat attacttgca
     2941 gtaacatttg caggaacact ggtttatggc agggacatgc ctgttatccg gatgaacgaa
     3001 cccggtcagg cgcctggaga taaccctgca cttgcaggca aagtaaaaga attgcttgaa
     3061 atacagaaga aaatgtttga cattgaaaga actgtgattc agcaagatca agaactcaaa
     3121 caactggcag aacagataaa agttcttcag acacaattac gaaacaggat agaagaaaaa
     3181 ttgaaagata acatggatta tcaaacgctg aaacagcgtc gtgaacagat ccgactggaa
     3241 taccagagaa cagattttaa aaaaatagat aatttcaaaa aaacacaacc ttaatgcaat
     3301 gtccagatag tttcttcctt ccgacaaaaa tgttatcata attttatgtt tggtggactg
     3361 gatctcatag taataatgtt ttttctgggt ggaatcctcg gaggagcagg caggggattc
     3421 ctcggcacag taattgatat ttgtggtatt attttcggta tttttattgg ttcatttgtt
     3481 tatgcggcgc ccgttaaact ttttgcaaag tttggtatca caggtacacc tgttgaactt
     3541 atttttttca tcctttcaag tattgttttt gcattgacct ttattttatt acttgaggca
     3601 ttaagaaaaa aggttgaaat caagccagcc attgatagaa tttttggagg catattcggt
     3661 agtatcaatg gatttgtagc agctgccagc attcttgtgg ttatgagttc atctgttcgc
     3721 atgggacagg aaatagacca ggcaaaattt gcgccacgtg taagggacgg attgctaaag
     3781 ttttatgaaa agattgagcg acacaacttt acacttccaa agattattat tcttcctgtt
     3841 gcctataagg atgaatttgg aaacaatgtt aaaaccgcaa aattcgttaa gatgaacttt
     3901 acaaaatttg aagggtacac ctgtattaac tgcggcggaa aggtgaaatt tgctggttat
     3961 tttacaaaat acggagttgg acttgtgccg aaactggtat gtgaaaaatg tggtcgcaca
     4021 tcagacggtt gccagacata tgaaggatat cacaaacttt acaatgcctg cgtggtcgaa
     4081 cttgcacgtc aggggatgaa atttgactgt ggtacatggc caaatcatac atgggttacc
     4141 cccacaggac cgtgtcctgt tgacaataat tctcttgaat tgtttttatg gcgcgaacca
     4201 attaaatatt gattctgatg acaccagttc ataccattga atctttgtta accggtttaa
     4261 tcgcaggttt tatctgcggt ttaacaggta tgggtggcgg tactatttat gtaccagtgt
     4321 tctattttct gtttggtttc tcaataaaaa aggcaattgg aacaagtttg ctcgtaatat
     4381 ttatttcgtc tgtttctgct ttttttgccc atgccagggg caggcagata gattttaaga
     4441 tgtcaatcta tttagtcgtt tctggaatta tcggtgcgca aatcggaagc gcaattactg
     4501 cacgacttcc ggatattgca gtgaaaattt tctttatatg tttaacaatg tttctttctt
     4561 ttaacatgtg gcgggataga aagaatgagg tggaaagttg ccccgcgaat atgaactttt
     4621 caccggttaa attgacattg atcggattga ccggtggagt tatctcaggt atgggaggag
     4681 tgggtggtgc cattgttctg gtgccactgt tacatttatg ttgtggtgta cctatgaata
     4741 tttgcattgg cacaacactg tcggttgtga tatttaattc cctctcaggt gtgataggct
     4801 atttttatca caaccttgtg gatgttcaaa cagggacagt aacaggaatt gtggctgttt
     4861 tcactgctgt tgcgggcgca aaactcagta tgaaaacacc cactcgaaaa ttgagaaaaa
     4921 tattttcagt gatacttcta atggcaggac ttgctgttgt tttcggaaga taacattgtt
     4981 ttgatttgtg gtaatctggt ataataaaac catggaacag aaatcaaaag cattggcagt
     5041 actcaaagaa aatgaactga ttgaaagggc aaaaaataaa gaccatgctg cgtttgaaga
     5101 actggtgaaa aggaatcagg cgcggattta taaccttggt ctaaaactcc ttggcaacaa
     5161 agaggatgct gctgaccttt tacaggagac cttcatcaat gcatactatg cactcccaaa
     5221 gtttgaaggc aaatctgcct tttcaacctg gctgtacaga attgccacaa acaatgcttt
     5281 catgaaaatg agaaagaaaa aaatcccact tgtccgattt gaatcagaca gggaagattc
     5341 tgaacaacga tatttcactg atgtgcctga ctggtcaaac gaccccaggg ctcatttgaa
     5401 aaatgaggaa cttaaggtgc tgcttgaaaa atcagttgat tctcttcccg gtaaatacaa
     5461 gacaattttt gttctccacg atattgaagg tttttccata gaagaaatca gcaaaatgct
     5521 tgatttgtct gtgcctgcgg ttaagtcaag aattcataga agcaggatgt ttttaaggga
     5581 taaactctct caatatttcc agttacatgc gggacaggga atacgggtat gaaatgtaaa
     5641 aaatattgtg aattgatttc tttgtatctt gacagggaac tggaccagat gcttattgat
     5701 gagcttgaag aacatctttc attatgcagg aattgtctgg ctttttttca tacgatggaa
     5761 aaaacggttt cgctgtccag aacatattac aaatacaggt gttgtaaggt tcccaggaat
     5821 gtttcctcac agatatttta caggcttcgg attatataca aagaagaaaa attttaggcc
     5881 tcttctcaaa tgaaatactc aatcaagggc tgggttaaga actctttttg tgactgggac
     5941 tcaaaggttt cctcagtgat ttttctacca caatgcaata tgcgatgtcc tttctgtcag
     6001 aatcaggaac ttgtagagcg ttacgatgac cttgaagata ttgactggca aactattaaa
     6061 aattatctta aagaaaacag ggattttctt gatggcattg tcattacggg aggagaacct
     6121 tttgtgtcag attttctttt tgatcttgtc aaagaggtga agacaatggg tttgggtatc
     6181 aagattgata ccaatgggac tttcccggaa caactgaaca cattgataga tacagggatg
     6241 atcgattgtg ttgcaatgga tgtaaaaaat gcattaaaca cagatgccta ttcaaaagcg
     6301 tgtggcataa acattactga tgggatgctt gaaaaaatag ttcaatcaac aaaagttttg
     6361 atgaattcag gtattgatta tgaattcaga acaacaattg ttcgtagttt gcactcatca
     6421 gaagatatca ggggaatcgc aaaatattta aatggagcca aaaagtatgt tcttcaacag
     6481 tatagaccga tgggtgtaaa aaaaggtttt gatggtggag aactgttctc aaaagaagaa
     6541 cttgacaatt tatgtaagga tatttcacaa tttttccagc aatgttttgt gagattctat
     6601 ggctgaactc atcggaaaca taaacgtttc atggtatgcc tgggtcggag taagttttct
     6661 tgccggaatt atttcttttt tttctccctg tgtgttgccg cttattccgg catatttctc
     6721 tttcattctt ggttcttcag tgtacgaacc tgaaaagagt aaaggacacc ttatacggtc
     6781 tgtcatactt atgtgctgtg gttttaccat tgtttttgtt actcttgggg cttctgctac
     6841 aggcattggc aggttccttg cgtctcatat gagttttttt aagttactgg caggaattat
     6901 tatgcttgtt tttgctcttg aaacccttga attgacacat attttttcat ttcacaaagg
     6961 tttttctcct gaattcagga aaaaacctgt gggtttgaac gcgataattt ttggcgttgt
     7021 actcgggatt tcttggactc catgcgtggg accgatactc ggtgcgatac tcacgatggc
     7081 tgccacacag gaaacagtag caaaaggtat actgctttta tttgtatatt ctcttggttt
     7141 atctgtacca ctgattattt ttgctgtatt tttttcacag atgaaaaaat ttcagaaatt
     7201 tatgatagtt catgccagaa aaataagaat tgttgctggt agtgtcttac ttctttttgc
     7261 tttttatctt ctttcccggg ggcgaatatg gatgtgattg ttattggggc tgggccggcg
     7321 ggtatttctg cgggtattta cgctgcaagg gcaaatatgt cttttatggt tattgaaaaa
     7381 atgtatcctg gggggaaact ggtatggatt gaaaaaatcg aaaattatcc aggatttcca
     7441 gatggtatcc caggacctga tcttgcagaa agattttata gacaggcaac agcactgaag
     7501 gtaaacttcg tttttcaaaa tgttgacagg atagaaaaag ttcaagaaca tttcagggtt
     7561 ttttctggta atgagtatta tgaagccaga tatattattg ttgcttctgg gtctgttccc
     7621 aagaaacttg ggattaaagg agaagacaca tttgttggca gaggtgtatc atattgcgct
     7681 atctgcgatg gggctttttt cagaaacaaa agagttgctg ttattggtga agggaaacat
     7741 gttatcacgg acattaacta tcttaaaggt cttgcatctg tgacatggat tagaaaaccc
     7801 acaggtcaat cccagaacgt ggaaggcgtg aatattatta acggggttcc ccttgaaatt
     7861 gcaggcgaag gtgttgtcaa aggtattgta gtacaaactg aaaaaggaat aacaacggtt
     7921 gaaacggatg gggtttttat atttgccgga ttcaagccct cttttgaatt cctccctcca
     7981 gaagtgacac tggaccaatc gggttacata aaaacagata gcactcttca gagttcaatg
     8041 aacgggattt atgtctgtgg agatattcgg tctgattctt tgaaacaaat tgtatctgcg
     8101 gtcagcgatg gagcagtggc tattaatgaa attagaaaaa aattatagat caatccgtct
     8161 tgctaacctt ttcaattttg tcacgggtct gttgaaggtt tgttttaagt ttttcaaatt
     8221 cagcaatcat cctgtcaact tcagcaatgg tctggtcaag attctttgaa ataccaccaa
     8281 tggcttgaat ctgggaatcc aactgttcaa tacgcgtttg aagttctgcc attttctttt
     8341 ttgaatatgc gtattcgcca ataaatgcaa cccatcctga gagaatcaaa atgacacccg
     8401 cccagatcaa taattttatt tttttcattt tttctctggc tccattgata ttgcgtcttt
     8461 tggacattcg tgcgcgcaaa tcccacaacc cttgcaataa tcgtagtcaa attcaaaaaa
     8521 ctttccatcc tttgttttga tagcagaatc cggacaataa acccagcaaa taagacagtg
     8581 aatacacttt tctggaatat gaaccggtct tacgcttctc cagttacccg ttttaaattt
     8641 tttcgatgta cctgcttcca gaacatcacc ttctggtagt tgcttccagg ataatttttt
     8701 ttcttccacc agtcacctcc atcagtttga tattttcacc tggttatatg ccatttcaat
     8761 tgctttgata tttccttcaa taatttctgg tctgttcctg aactttgcct caagtttgtg
     8821 tttaatgtct tcaataactt ctttgatgtc aagcattccg gtgaccttga taagagcgcc
     8881 catcattgga gtattcggaa tttctctgcc aattgtttca atggatattc tggatgcatc
     8941 cactgtacag atacgaccct taaattttaa actttcggca aattctgatg gttcttttga
     9001 agaattaaga agtataatac ctttttcagg ctccagacct gatattacat ctacctggtc
     9061 aacaagtgat acatcaagca caactactac atcagggttc aaaataggac cgtgagttct
     9121 gatagggctt gaactgattc tgttgaagga cacaacagga gcacccattc tttcggggcc
     9181 atattcagga aaggcctgga tgtatttgcc tgtggaacag gcagcatctc cgaaaagaag
     9241 tgctgcagtt tttgcaccct gtccgccacg cccatgccag cgaatttcaa acatttcttt
     9301 catgtggcat ctccttttcg taaagcaaaa gaa
//
LOCUS       DAORYU010000029         9068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_5646_length_9068_cov_3.955842, whole genome shotgun sequence.
ACCESSION   DAORYU010000029 DAORYU010000000
VERSION     DAORYU010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9068
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5646_length_9068_cov_3.955842"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /gene="rpsM"
                     /locus_tag="PLW07_01750"
     CDS             <1..245
                     /gene="rpsM"
                     /locus_tag="PLW07_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOL49278.1"
                     /translation="FVQQNFKIEGDLRREFGGNVSRLIEINCYRGARHRRSLPVRGQR
                     TRTNARTRKGPRKTVGVVRDKAARKAIRSNVGESGS"
     gene            266..667
                     /gene="rpsK"
                     /locus_tag="PLW07_01755"
     CDS             266..667
                     /gene="rpsK"
                     /locus_tag="PLW07_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOL49279.1"
                     /translation="MADKEKKKYTKKKKKHVSIPSGVAHIKATFNNTTITITDPEGRV
                     VAWASAGAVGFKGTRKGTPFAAQLIAENVAKKVQNLVNMRDVEVRVKGPGPGRETAIR
                     ALQTAGLSITAIRDVTPIPHNGCRPPKRRRV"
     gene            680..1297
                     /gene="rpsD"
                     /locus_tag="PLW07_01760"
     CDS             680..1297
                     /gene="rpsD"
                     /locus_tag="PLW07_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOL49280.1"
                     /translation="MAKITKPVCKVCRRNAEKLYLKGGRCESDKCPLDPRKQRKGMMA
                     AKTSEFGLQLREKNKVKSYYGVFEKQFRKYFSMAKKMKGVTGEILLRLLECRLDNVVY
                     KLNWAFSRRMARQMVSHGHVFVNGKRVNRASYQVKIGDEISIGDNSRFLEIAKNARET
                     GRATPVPVWLEASENEMKAKVVRYPTRDEVSIPVDEHLIINFYSK"
     gene            1308..2285
                     /locus_tag="PLW07_01765"
     CDS             1308..2285
                     /locus_tag="PLW07_01765"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003519.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOL49281.1"
                     /translation="MEEKSIEFLYPEKIAWEKGTYKSNYGQLIVEPLEKGFGVTVGNA
                     LRRVLLSSIPGVAITGIKISGVQHEFTAIEGVKEDTTQIVLNLKQVICKPIISEFPHR
                     CSVTISGMSELCAKDLITDGSVEILNPDLHIATLDPSTKLTIDFEITEGRGYLPVEKM
                     KLIRKDIPADMILIDGIYTPVRKVAFHIENTRVRQLVDYEKLILDVWTNGAITPADAL
                     IKTTNILNRHFMTIGTMQMIGDQPVSVPEKKQVDILDTPITELKLSTRVINALQTHNI
                     STLREILEMSRTEFEEMKNLGKKSIQEIEEVLEKKGYKLKIDSSDTEQS"
     gene            2282..2632
                     /gene="rplQ"
                     /locus_tag="PLW07_01770"
     CDS             2282..2632
                     /gene="rplQ"
                     /locus_tag="PLW07_01770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOL49282.1"
                     /translation="MRHKKNIRKFGRNKSHRRSLLRNMTISFFQNKKVMTTEAKAKQL
                     KRVVERLITAGKKQDLVSIRQINSYLNHPATTKIVMDLGKKFEGRKGGYTKIIKVDRR
                     RGDASKMAIIQMVE"
     gene            2634..2849
                     /locus_tag="PLW07_01775"
     CDS             2634..2849
                     /locus_tag="PLW07_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49283.1"
                     /translation="MKDSTKILDRYDKKENIYQLILKFSEKGHKILAGGTASVSDDSL
                     NVLDVVLGDIESEKETDEHREAVEKDG"
     gene            2815..3912
                     /locus_tag="PLW07_01780"
     CDS             2815..3912
                     /locus_tag="PLW07_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOL49284.1"
                     /translation="MNIEKLLKRMADLDASDLHLKVGRKPFFRVKTVLQESAEDDILT
                     PQEIENYAFSIMNEKQKILFLEQMEMDLATESPETGRFRVNIFRQRGYVGIVMRRIKS
                     TIPSFDQLGLPPVLAKISDFPTGLILVAGPTGCGKSTTLAAMIEYINQNRQALHIITI
                     EDPIEFVYTDKNCIINQREVGLDTLSFHDALKRVLREDPDIILIGELRDTETFEAAIR
                     ACETGHLVFSTLHTVDTITTINRILDFFPQQQHDMVRKTLAYNLRATCCQKLLPRKDG
                     TGMVPVCEVMVVTPIISRLIMENKMGKIPSAISSDKETGMQTFNQHLVKLINEGKITR
                     ETGFEASPAPNALEMNLRGIYLDEDTRIIGD"
     gene            3921..4181
                     /locus_tag="PLW07_01785"
     CDS             3921..4181
                     /locus_tag="PLW07_01785"
                     /inference="COORDINATES: protein motif:HMM:NF014857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase family
                     protein"
                     /protein_id="HOL49285.1"
                     /translation="MKILVIGSGGREHAICWKIASEIKTGTLYALPGNGGIGEIANLV
                     DISIDQLQNISSFAKKEKIDLTIVGPELPLASGIVDEFKKRD"
     gene            4178..5179
                     /gene="purD"
                     /locus_tag="PLW07_01790"
     CDS             4178..5179
                     /gene="purD"
                     /locus_tag="PLW07_01790"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOL49286.1"
                     /translation="MKIFGPSQKAAKLESSKVWAKEFMIENDIPTGHFSVADNFNQAK
                     AILERVMFPVVIKFDGLAAGKGVSVATEMSQAISFIEDVFEKRVFSAENNRVVIEEFL
                     SGEELSYLVITDGENYIPLLPARDYKKIYDGDTGPNTGGMGCYSPVSMCGPELESFIE
                     KKIVIPTIKGLQKKGIDYCGVIYCGLMITEKGPCVLEYNVRFGDPETEVVLPRMAGGL
                     LEVLEAATEKLLGKTSIKWNTDAAVDVVIASGGYPGKYETGYSINGLDSVPTNVVIFH
                     AGTRKENDLFITAGGRVLNVVGLGKTIAQARTKAYEKVNAISFKNMYYRRDIASFPK"
     gene            5277..5540
                     /gene="rpsO"
                     /locus_tag="PLW07_01795"
     CDS             5277..5540
                     /gene="rpsO"
                     /locus_tag="PLW07_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOL49287.1"
                     /translation="MEKSVKEKLRQDFGRHPQDTGSPEIQIALLTRRIEALGEHFKKY
                     PKDFNSRRGLLKLIGHRRRLLKYLQTKDLEAYQALIEKLNLRK"
     gene            5545..7680
                     /locus_tag="PLW07_01800"
     CDS             5545..7680
                     /locus_tag="PLW07_01800"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOL49288.1"
                     /translation="MTGKLEIELGGKPIILETGFLAKQAAGSVTVSCGDTVVLVTVTT
                     KKSEEIRDFFPLTCDYREQTSAAGKIPGGFFKREGRPSEREILISRLIDRSIRPLFPE
                     DFADEVQIISFVLSVDQENDPDILSVIGASGAILLSGLPFSGVLGCVRMGRIDDKFVI
                     NPTISELEKSTINLVLSGTESSIVMLEGWAKELSEDLFIDATRFAFDAIKPLIDSLSL
                     WKQKIDYKPEIEFDPDTIDSVTSFIKKKMESIWDYPEKKQRNQYYENMLVELKTSYDE
                     EKHDEIELIFKKCLEKTIRESILLTGKRFDGRKPDEIRPIECKVGVLPRTHGSALFTR
                     GQTQCLASVTLGTKHDQQIIDGLFEETSKRFMLHYNFPPFSVGETGALRGPSRREIGH
                     GALAEKSLQFLISDETEFPYTIRIVANILESNGSSSMATVCASSLALMDGGIPLPKHV
                     AGIALGMIKEKEKYIILTDIAGEEDHVGDLDLKIAGTSSGITAFQMDVKTTEFSWEIL
                     KEAVLQSKQAREKILDIMNGVLISPRPEVSKYAPRIISFKVSPDKIGLVIGGGGKTIR
                     KIIEDTGAEIDIEDDGTISIYSPEIEKCQAALERIKAIVEEPEVGKVYEGIVTRIFPF
                     GAMVKFFNEQEGLVHISELAPYRVNKVEDVVGIGEKIKVKFVGFDEQGRANLSRKQAL
                     TQEEIEQEKKKSTFHPRSFKRDSHREPRK"
     gene            complement(7683..8372)
                     /gene="cmk"
                     /locus_tag="PLW07_01805"
     CDS             complement(7683..8372)
                     /gene="cmk"
                     /locus_tag="PLW07_01805"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOL49289.1"
                     /translation="MSHKHFVIAIDGPAGAGKSTVAMLVAKTLGFLYVDTGAMYRALT
                     LKALQLQLNFNDKNRLIEMSRETRIELLQDGNGYSVFLDGKDVTKTIRTEQVSKNSHF
                     IASIPEIREILWNIQRSYREKYDIVMEGRDIGTVVFPDAQLKIYLDASVEERAKRRYL
                     QLKQQGIEMDIENLKQEIILRDERDKNRKIAPLKKHSDAITIDTTCLTIEQEVSMIVQ
                     LYRQMIEKIQE"
     gene            complement(8362..8919)
                     /gene="frr"
                     /locus_tag="PLW07_01810"
     CDS             complement(8362..8919)
                     /gene="frr"
                     /locus_tag="PLW07_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOL49290.1"
                     /translation="MIKEICQEAELNMKKVEDHFIREINNFRLGRASVTLLEGILVEY
                     YGSRLPINQIATVSIPNPQSLVIQPWDKSTIDKIVKAIQMSNLGLNPVADSSTIKISV
                     PPLSEERRKELVKILRKLEEQAKVEMREIRRKVKEEIKKLEKEKKISEDDSFKATEKL
                     QELTDNFIEEIEKKTSVKEKEILES"
BASE COUNT         2861 a         1548 c         2063 g         2596 t
ORIGIN      
        1 cgtttgttca gcaaaacttt aaaatagaag gtgatttaag aagagaattt ggcggtaatg
       61 ttagcagact tattgaaatc aactgttatc gtggagcccg acacagacgt tcattgcctg
      121 ttcgtggaca gagaacaaga acaaacgcga ggacccgcaa gggaccaagg aaaactgttg
      181 gtgttgtcag ggataaggct gcaaggaaag cgataagatc caatgttggt gaaagcggaa
      241 gttaattctg ttataaggag agaaaatggc tgataaggaa aagaaaaaat acacaaaaaa
      301 gaagaagaaa catgtaagta ttccgtccgg ggttgcacat ataaaagcaa ccttcaataa
      361 taccacgatt accattactg atcctgaagg cagggttgtg gcatgggcaa gcgctggtgc
      421 agtcgggttt aaaggtacga gaaagggtac cccatttgct gcccagttga tcgctgaaaa
      481 tgtggcaaaa aaagtccaga atcttgtgaa tatgagagat gttgaagtaa gggtgaaagg
      541 tcctggccct ggtcgtgaga cagcaataag ggctttacag acggcagggt tgagtattac
      601 tgcaataaga gatgttacac cgataccgca taatggttgt agacctccca agagaagaag
      661 ggtataaaaa ggagttttca tggcaaaaat cactaaaccc gtatgtaaag tttgtagaag
      721 aaacgcagaa aaactgtact tgaaaggtgg aagatgcgaa agcgataagt gcccccttga
      781 tccacgaaaa caacgtaagg gtatgatggc agccaaaaca tctgaatttg gcttgcagtt
      841 gagagagaaa aacaaggtaa aatcttacta tggcgttttt gaaaaacagt ttagaaaata
      901 tttctcaatg gcaaagaaaa tgaagggtgt aaccggtgag atacttttgc gcttgcttga
      961 atgtcggctg gataatgttg tttataaatt gaactgggca ttttctcgta gaatggcaag
     1021 acagatggta tctcatgggc atgtttttgt aaacggcaaa agggtgaaca gggcatcata
     1081 tcaggtgaaa attggcgatg agatcagtat cggagataat tcaaggtttt tggagatcgc
     1141 aaaaaatgca agggaaactg ggagggcaac tcctgtacct gtatggcttg aggcttctga
     1201 gaatgaaatg aaagcaaagg ttgtgagata tccgacgaga gacgaagtca gtatacctgt
     1261 tgatgagcat ctcatcatca acttctattc aaagtgaggt ggatattatg gaagaaaaat
     1321 ctattgagtt tttgtatcca gaaaaaatcg catgggaaaa aggaacatat aaatctaatt
     1381 atggacagtt gattgttgaa cccctcgaaa aaggttttgg tgtgacagtt ggaaacgcac
     1441 tgagaagggt tttgctttct tctattcccg gggttgctat tacaggaatt aagatttcgg
     1501 gtgttcagca tgaatttacc gcaattgaag gtgttaagga ggacaccacg cagatcgttc
     1561 ttaatctgaa gcaggtgata tgtaagccga ttatttctga atttccgcac aggtgttctg
     1621 taacaatttc tggaatgtct gaattgtgtg caaaagatct cattactgat ggaagtgttg
     1681 agattttgaa tccggacctt catattgcga cccttgatcc atcaacgaaa ttgacgattg
     1741 attttgaaat aaccgaggga cgaggctatc ttcctgttga aaaaatgaag ttgatcagaa
     1801 aggatattcc agcagatatg attctgattg atggaatata tacaccggtg aggaaggttg
     1861 cttttcatat tgaaaataca agagtcagac aacttgttga ctatgaaaaa cttattcttg
     1921 atgtgtggac aaatggtgca ataacaccag ccgatgccct tattaagact acaaacattc
     1981 ttaacaggca ttttatgaca atcgggacaa tgcagatgat aggggaccag cctgtatcgg
     2041 ttccagagaa gaaacaggtc gacatactcg acacgcccat tactgaattg aagctttcca
     2101 ccagagtgat taacgcgctt cagacgcaca acatcagcac tctcagagaa attcttgaga
     2161 tgtcccgtac agagtttgaa gagatgaaaa atcttgggaa aaagtccatc caggagatag
     2221 aagaggttct tgaaaaaaag ggttacaaac tgaagataga ttcgtctgac acggagcagt
     2281 catgaggcac aagaaaaata ttcgtaaatt tggacggaac aaatctcatc gtagaagtct
     2341 tctgagaaac atgaccatca gtttttttca gaataaaaag gttatgacca cagaagcgaa
     2401 agcaaaacaa ttaaaaaggg tggttgagag attaattact gcagggaaaa aacaggatct
     2461 ggtaagtatt agacaaatca attcatatct taatcatcct gctacgacaa aaattgtgat
     2521 ggatctgggt aaaaaatttg aaggaagaaa aggcggctat acgaaaatta tcaaggttga
     2581 caggcgtcgc ggggacgcaa gtaaaatggc aataatacaa atggtggaat aatatgaagg
     2641 attcgacaaa aattctcgat agatacgaca aaaaggaaaa tatttatcag ttgatattga
     2701 aattctctga gaaaggacac aaaattctcg cgggtggtac agcgtctgtt tctgacgatt
     2761 ctttgaatgt gctggatgtg gttctggggg atattgagtc cgagaaggaa acggatgaac
     2821 atagagaagc tgttgaaaag gatggctgac cttgatgcgt cggatcttca tttgaaggtt
     2881 ggaaggaagc ccttttttag agtcaagaca gtcctgcaag aatctgcaga agatgacata
     2941 ttaactcctc aggaaattga aaattacgct ttcagtatta tgaatgagaa acagaagatt
     3001 ctgtttcttg aacagatgga aatggacctt gccacagaaa gcccggaaac aggcagattt
     3061 cgtgtaaaca tttttcggca gagaggatat gttggtatcg ttatgcgcag aatcaaatcc
     3121 actataccat catttgacca gcttggttta cctcctgtgc tggcaaaaat ttcagatttt
     3181 ccaacaggcc tcattcttgt agccggtcca acggggtgtg gtaaatcgac cactcttgca
     3241 gcaatgatag aatacattaa ccagaataga caggctttgc acattatcac aattgaagat
     3301 ccaattgaat ttgtctacac tgacaaaaat tgcatcatca atcaacgtga ggtggggctg
     3361 gatacgcttt catttcatga tgcattgaaa agagttctgc gcgaagaccc ggacatcatt
     3421 ctgataggcg aattgcgcga tacagaaacc tttgaagctg caattcgtgc atgcgaaacc
     3481 ggacatcttg ttttcagcac cctccataca gtagatacaa ttaccacaat aaacagaatt
     3541 cttgattttt tcccgcagca acagcatgat atggtaagga aaactcttgc ttacaacctt
     3601 cgtgcaacat gctgtcagaa attgctgccc agaaaggatg ggacgggtat ggttcctgtt
     3661 tgtgaggtta tggttgttac gccgattata tcgagactta ttatggaaaa taaaatgggc
     3721 aaaataccga gtgcgatatc ttcagataag gaaaccggta tgcaaacatt taatcagcat
     3781 cttgtaaaat tgattaatga gggtaagatt acaagggaga ccggttttga agcgtcgcct
     3841 gcgcccaatg cccttgaaat gaatctacga gggatatatc ttgatgaaga caccagaatt
     3901 attggagatt gaggtgtgac atgaaaatac ttgtgattgg aagtggtggc cgcgaacacg
     3961 caatatgctg gaagattgcg tctgaaatta aaacaggtac actttatgcg cttcctggaa
     4021 atggaggcat tggagaaata gcaaatctgg ttgatatctc tattgatcaa ttgcagaaca
     4081 taagttcctt tgccaagaaa gagaaaatag accttactat tgtcggtcct gaattgccgc
     4141 ttgccagtgg tatagttgat gaatttaaaa aaagagattg aagatttttg gcccttctca
     4201 aaaggcagca aaacttgaat cgtcaaaagt gtgggcaaaa gaatttatga tagaaaatga
     4261 tattcccaca ggacattttt ctgtggcaga caattttaac caggcgaagg cgattcttga
     4321 aagggttatg tttcctgttg taattaaatt tgacggtctt gctgcgggca agggcgtgag
     4381 tgttgccact gaaatgagcc aggcgatatc atttattgag gatgtttttg aaaaacgcgt
     4441 tttttcggct gaaaataaca gggttgttat agaggaattt ctttctggag aggaactatc
     4501 atatcttgtg ataacagatg gggaaaacta tattccgcta ttgcctgcaa gggactataa
     4561 gaagatttac gatggggata cgggaccaaa tactggaggt atgggttgtt acagccctgt
     4621 ttcaatgtgt ggtcctgaac ttgaaagttt tatagaaaag aaaatagtga ttccaacgat
     4681 aaagggtttg cagaagaaag gtattgatta ctgtggagta atttattgtg gattaatgat
     4741 aacagaaaag ggtccctgtg ttcttgaata caatgtaaga tttggagatc ccgaaacaga
     4801 ggttgtttta ccaagaatgg caggtggttt actcgaagtg ctggaggctg caacagaaaa
     4861 acttctcggc aaaacatcta taaaatggaa cacagatgct gcggttgatg ttgttatcgc
     4921 ctcaggggga tatccgggaa aatatgagac cggatattcc attaatgggc ttgattctgt
     4981 tccaacgaat gttgtgattt ttcatgcggg aacaaggaaa gaaaatgatc tttttattac
     5041 cgcgggtggc agggtattga atgttgttgg tctgggtaag acgattgctc aggcaaggac
     5101 aaaggcgtat gaaaaggtta atgccatttc ttttaaaaac atgtactaca ggagagatat
     5161 cgcctctttt ccaaagtaga attcattgac aaaatttcaa taacattata tacttagtat
     5221 cagtgaaagg cgattcaaag agtttcagca caacacatca aggagaaatg gaaatgctgg
     5281 aaaaatcagt aaaggaaaaa ttaagacagg attttggacg acatcctcag gatactgggt
     5341 cccctgaaat ccagattgcc ctgttaacaa gaaggattga agcgcttggt gagcatttca
     5401 aaaaataccc aaaggatttt aattcaagac gaggcttact gaagcttata ggccacaggc
     5461 gacgtctgtt gaagtacctt cagacaaaag accttgaggc ttaccaggct cttattgaaa
     5521 agttgaattt gagaaaataa aaaaatgaca ggaaaactgg aaatagagct tggcggtaag
     5581 cccataattc ttgagaccgg gtttctcgca aaacaggctg caggttcggt aacagtaagt
     5641 tgtggtgaca cggttgttct tgttacagtc acaacaaaaa aatcagaaga aattcgtgat
     5701 tttttcccct tgacatgcga ttatcgcgaa cagacgtctg ctgcaggtaa gatccctggt
     5761 ggattcttta aaagagaagg tagaccatca gagagagaga tactgatcag ccggttgatt
     5821 gatcgttcaa tcagaccttt gtttccagaa gattttgcag acgaggtgca gataatttcc
     5881 tttgttcttt ctgttgatca ggaaaatgac ccggatattc tttctgttat tggtgcgtcc
     5941 ggtgctattt tgctttccgg attacctttc agtggagttt tgggttgtgt gcggatggga
     6001 agaatagatg ataaattcgt aataaaccct acgatttctg aactggaaaa aagtaccata
     6061 aatcttgttc tttctggcac agaaagttca attgtcatgc tcgagggctg ggcaaaagag
     6121 ctttctgaag acctgtttat cgatgcgacg cgatttgctt ttgacgcaat aaaacctctt
     6181 attgattctt tatcattgtg gaaacagaag attgactaca aacctgaaat tgagttcgat
     6241 ccggatacaa ttgacagcgt gacatctttt ataaagaaga agatggaatc aatatgggat
     6301 tatccggaaa aaaagcaacg aaatcaatat tacgagaaca tgctggtaga attgaagaca
     6361 tcctatgatg aggaaaaaca tgacgagatt gaattgatat ttaagaagtg tcttgaaaaa
     6421 accatcagag agtccatact gttaactggt aaaagatttg acggtagaaa acccgatgaa
     6481 atcagaccca ttgagtgtaa ggttggggtc ttaccacgaa ctcatggttc tgcactattt
     6541 acccgtgggc aaacccaatg tctcgcttca gtaacacttg gtacaaaaca tgaccagcag
     6601 attattgatg gtctttttga agaaacatca aaaagattta tgcttcatta caattttcca
     6661 ccattttctg ttggagaaac cggtgctctt cgtggaccat ctcgaagaga aattggtcac
     6721 ggagcacttg ctgagaagtc ccttcagttt cttatatccg acgaaacaga atttccttac
     6781 acaataagga ttgtggcgaa tattctggaa tcgaatggtt cttcatctat ggcgactgtt
     6841 tgtgcgtcaa gtctggcatt gatggatgga ggaataccgc ttccaaaaca tgttgcggga
     6901 atagcactgg gtatgatcaa agaaaaagaa aagtacatta ttttgacgga tatagcgggt
     6961 gaggaagacc atgtaggaga tctggatttg aaaatagccg ggacttccag tggaattact
     7021 gcttttcaga tggacgtaaa aacaactgag ttttcatggg agatcctgaa ggaggcagtg
     7081 ctgcagagca aacaggcaag agagaaaatt cttgatataa tgaatggagt tttaatttct
     7141 ccaagacctg aagtttcaaa gtatgcgccg agaattattt ctttcaaggt ttctcctgat
     7201 aaaattggcc tggttatagg cggtggcggt aaaacaatca gaaagattat tgaagacaca
     7261 ggggctgaga ttgatattga agatgatgga accatttcta tttatagccc tgagatagaa
     7321 aaatgtcagg ctgcgctgga acgaattaag gcaatagttg aagaacctga ggttggaaag
     7381 gtatacgaag ggattgttac aagaattttc ccgttcggtg cgatggtgaa atttttcaac
     7441 gaacaggaag gccttgtcca tatttcagaa ttggcaccgt acagggttaa taaagttgaa
     7501 gatgttgttg gcatcggaga aaaaatcaaa gtgaaatttg ttggttttga tgaacagggt
     7561 agggcaaatc tgagtcgcaa gcaggcgcta acacaggaag aaattgaaca ggaaaagaaa
     7621 aaatcaactt tccatccccg ttcattcaaa agggattccc acagggaacc cagaaaatag
     7681 atttattcct gaatcttttc aatcatctgc ctgtaaagtt gaacaatcat tgacacttcc
     7741 tgttcaattg taaggcatgt tgtatctatt gtaattgcat cagaatgttt tttgagtggt
     7801 gcgatttttc tgtttttatc tctttcatct ctgagaatga tttcctgttt caaattctct
     7861 atatccatct caattccctg ttgtttgagt tgtagatacc ttctttttgc cctttcttca
     7921 acagaagcat caagatagat ttttagttga gcatccggga aaacaacagt accaatatcc
     7981 ctgccttcca ttacaatgtc gtatttttcc cgatatgaac gttgtatatt ccacagtatc
     8041 tctctaatct ctggaattga tgcaataaaa tggctgtttt tgctgacctg ttcggtacgg
     8101 atggtttttg tgacatcctt tccgtcaaga aaaacactgt acccgtttcc gtcctggaga
     8161 agttcaattc gggtttccct gctcatttct attaatctat tcttatcatt aaaattcagt
     8221 tgtaactgaa gcgcttttaa tgtgagtgcg cggtacattg caccagtatc aacgtagaga
     8281 aatccaagtg tttttgccac gagcattgca actgtgcttt taccagcacc agcaggtcca
     8341 tcaatggcga tgacaaaatg tttatgactc aagaatttcc ttttctttta cagatgtttt
     8401 cttttcaatt tcttcaatga aattatctgt caactcctgc aatttctcag tcgccttaaa
     8461 tgaatcatct tcagaaattt tcttttcctt ctcaagtttt tttatttctt ccttcacttt
     8521 tcttcttatt tccctcattt caacctttgc ctgttcttca agttttctaa gtattttgac
     8581 cagttccttt cttctttctt cactcaatgg tggtactgaa attttaattg ttgaagaatc
     8641 agctactgga ttaagcccga gattgctcat ctgaatggct tttactattt tgtctatggt
     8701 tgatttatcc catggttgaa ttacaagact ttgcggattc gggatagata cagtcgcaat
     8761 ttgattgatt ggtagtctgg agccataata ctccacgaga attccttcaa gcagtgttac
     8821 agaagcacgt ccaagacgaa aattgttaat ttccctgatg aagtgatctt caaccttttt
     8881 catatttaat tctgcttcct gacagatttc ttttatcatt ctgcctcctt tcaatgcaat
     8941 tgatataatt ttgaattttt atcagcgata atcgttcttg ttcccttacc aaatacaatc
     9001 ttctttaagt ttcctttttt aaaaaggttg aacacaatga ttggtaattt attgttcata
     9061 cacaaggt
//
LOCUS       DAORYU010000030         8822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_5813_length_8822_cov_3.717235, whole genome shotgun sequence.
ACCESSION   DAORYU010000030 DAORYU010000000
VERSION     DAORYU010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8822
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5813_length_8822_cov_3.717235"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..476
                     /locus_tag="PLW07_01815"
     CDS             114..476
                     /locus_tag="PLW07_01815"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOL49291.1"
                     /translation="MKKSSCSGFTIIEFLLAFVIIAIALIGTSAFYFASSRNLHQSDI
                     TRLATWKAIEKMEMIKSTDYSLIQNETENIIVGNVQVERITTVSEDTTLPLKTVEVKI
                     QKTDGTKIVGISTMIVRP"
     gene            473..934
                     /locus_tag="PLW07_01820"
     CDS             473..934
                     /locus_tag="PLW07_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49292.1"
                     /translation="MKILKIKGLGLIELMIVLVLGVLAIFAASSIVIESYREWKRSNE
                     VASLQVDFDLACHMIKSIIEEADTVQVSDNGTSITAGYQSYWIQQFYQDPQNPSVLVW
                     KNMKTNESQKVITTLKNISFDVQDDLVVVNIEVGNGTKSIAGSFSVYMRNS"
     gene            935..2224
                     /locus_tag="PLW07_01825"
     CDS             935..2224
                     /locus_tag="PLW07_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49293.1"
                     /translation="MKKGYILPMAIIFVVISLIMGMSILYLGGTAQISSMKRYHKEKA
                     FYIAEAGANWAFANKRANESWHPGAGTVSFDGGTFQINEIVSGETIIFESTGRYGNQE
                     STVSIVTSRTGGSGGGTGGSFGKGLFGSQSVTLYENAWVDSYDSRLGPYGPSNRGNHG
                     DTGSKNTVTLYNNAHIHGTAMVEDGPQYLYLGHNATVTDSDLYHSFEDPLNNLPAVVV
                     PSDLMNLLYPVQGDPRITGNYTISNGRLTVNNNRTVTISGGDFKFKSITMNNNSIMYI
                     TGNTRLYIESSLTLSNNTQVIIQNNSVVIWYLGNQGTPFTPTLANNSVINNTSQTTGN
                     LRIYVASSTNMTLANNAQAFNGVLYAPTSSITLANNSQFFGGIVSNVLTIVNNAVVHY
                     DVALREINFPEDPGLGTGPGTRMIVRWTKPDWSARLQ"
     gene            2253..3302
                     /locus_tag="PLW07_01830"
     CDS             2253..3302
                     /locus_tag="PLW07_01830"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOL49294.1"
                     /translation="MCELLFFSPTDRKYRRENRKWQGIPGITMTSDGTLWAVWYSGGD
                     GEGPENYVLVVNSKDKGINWSEPIIVIDPPDDIRAFDPCLWTDPYGKLWLFWAMSKNW
                     WDGKAGVWAITGEQVGDGCVWSKPRRIADGIMMNKPAVLSTGEWLFPIAVWNREPFLP
                     EMDNLRFSNVYISKDNGKTITFLGSADVPERMCDEHMIVELKTGVLRMLVRTSYGIGE
                     SFSYDRGKTWIKGCSSNIAGPGSRFHIRRLNSGNLLLINHYGFSEKNRSHLTAMLSED
                     DGKTWPYKLLLDERDKVSYPDAIETKNGEIFIIYDYDRYGKKEILMAVVTEQDIKAGE
                     IITPDSKLKMPVDDG"
     gene            3306..3932
                     /locus_tag="PLW07_01835"
     CDS             3306..3932
                     /locus_tag="PLW07_01835"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOL49295.1"
                     /translation="MENILSFLANFIVTIIEKTGYLGVFILMTFESALIPIPSEITMP
                     FAGYLVSINKMNIHILSFIGALANLVGSIAAYLLGFWLEDRLIRQWIKRRGKFLLISE
                     DDYDRAERWFRRYGDPVVFFSRITPGIRTFISLPAGVAKTNFGKFCVFTFIGSLIWSY
                     FLSYIGFFFGKNWSRIEIYYRKYEYLILFLIILLISWFIFSKVKKKKT"
     gene            complement(3933..4580)
                     /locus_tag="PLW07_01840"
     CDS             complement(3933..4580)
                     /locus_tag="PLW07_01840"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOL49296.1"
                     /translation="MAEKPDYERLRKFMVEHQIRARGIKNERVLSAFLKVPREEFVSN
                     QFKSSAYDDCPLPIDYSQTISQPYMVAIMTELTNPEKDDRVLEIGTGSGYQTAILCEL
                     GCRVFSVEKIPELAERAKKILEKLGYNANIMVGDGTLGWEQYAPYKIIIVTAGAPDIP
                     QSLIEQLDEGGRIIIPVGTLYSQDLIRAIKKKGKLIQENHGGCQFVPLKGKEGWE"
     gene            complement(4558..5253)
                     /locus_tag="PLW07_01845"
     CDS             complement(4558..5253)
                     /locus_tag="PLW07_01845"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOL49297.1"
                     /translation="MDKESIAVLIPAHNEARTIGKLVDNILEIVDYVCVVDDGSNDNT
                     GNIAKQHGAIVITHPVCKGKGAALKTGFQHFQNLPYDVIIAMDGDGQHPVSDLHVFIE
                     AIKKNKKAGIIVGKRKIIGSDMPPIRRLTNLSMSILISILAFQWIPDSQNGFRAIRKK
                     VVENMLIITNHFETETEILLRACWKGVKISSVPVSTIYQSEKSKIKPIRDTIKFFLML
                     LKICVYHGRKTRL"
     gene            complement(5263..6435)
                     /locus_tag="PLW07_01850"
     CDS             complement(5263..6435)
                     /locus_tag="PLW07_01850"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOL49298.1"
                     /translation="MGSRKTVVAIFFLWLFSKDGGQGVFLAPTEILAEQHYLNWSPFF
                     LSCGVESALLTGNLPEKMKKQVREKIEKNQIQVLFGTHALLNEKIVYPELKAIVVDEQ
                     HKFGVEQRNLLQKKSSTVHYLAMSATPIPRSVALAFYGNVDMSTIGQLPKGSRRITSY
                     LFKNEETEKVYEFIKRQVYDEKQGYFVAPTITGSEESSVVYHFNKISQIVEPQYVALL
                     HGKLPGHEKSKIIERFREKKIRIIVSTTVIEVGIDVPDANFIVIDEAEKFGLSQLHQM
                     RGRVGRSGEIGYCILIHHTDDPASVSRLESFLEIEDGLKLAEIDLSLRGPGDLLGVRQ
                     HGVLPLKIGNIVTDIEILNESRNEAERILNNKLYLKEKYVRIKEYLEHCIQSKIID"
     gene            complement(6445..7269)
                     /locus_tag="PLW07_01855"
     CDS             complement(6445..7269)
                     /locus_tag="PLW07_01855"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HOL49299.1"
                     /translation="MIPGLGLRRAEYFYKMGITTIKDLLFHFPRKYLDRRQIKKISEV
                     IPGETVSLKGNVMAVEEKRINRLSIVKIAISDGTGVIFLIFFNQNYILNILKPGTKVF
                     VYGRIDIYKENLQINNPLFEILETKRKLDYILPVYSVVSGLTQNFLRKCIRYALENMQ
                     QFPPDILPLDDRSRLKLSSMKHALINIHFPENQINLERARRHLIFDEFFRLQIGLIYK
                     KIITTGHIENISQNKFESSSVHNFAKMLPFQTTADQTKAMKEILKDLSEGRIINRL"
     gene            complement(7299..7838)
                     /locus_tag="PLW07_01860"
     CDS             complement(7299..7838)
                     /locus_tag="PLW07_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49300.1"
                     /translation="MSEVILELVEEFFQQRKYFTARSGNLVLVKKAKTEDNSDIGFIL
                     DETGVEYIPTAIIKPIAWHTCKITTRILETFPEILEFAKENKEKKFSEWFKGESFAKI
                     LVIPQLPSHIDLKQKVLEKLQSTGINHLITIPVILTGVINKIEARKVYSSPICDLLRV
                     LKFYKLISLSQEQMELPLR"
     gene            complement(7841..8644)
                     /locus_tag="PLW07_01865"
     CDS             complement(7841..8644)
                     /locus_tag="PLW07_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOL49301.1"
                     /translation="MKEFLIFFIGACFGSFANVCIYRLPRNKSIVSPGSFCPKCRRSI
                     PWYCNIPILSYLLLWGRCRYCKKTISPRYPIIELAMGLLTLLLYQKFWVAYLPYVFLF
                     YFIFAFGLVIISGIDFETFLVPDVIVLPLIPLGLIGALLCEGFFLPQNGSWSMVTRAS
                     YSLAGILIGGLLVAFLAVIGKIIWKKEAMGGGDLKLLAAIGAFLGWKGVFLSIFFGSI
                     LGTVISLALIYSKKKKWDDYVPFGPYLAIGAIIAILYKGNFFLSLYFIP"
     gene            complement(8641..>8822)
                     /locus_tag="PLW07_01870"
     CDS             complement(8641..>8822)
                     /locus_tag="PLW07_01870"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOL49302.1"
                     /translation="KIENPPPTKESAEKTYHIVKKGETLSSIAKKYGTTMKKIKEANN
                     MKTDIVTTGQKLLIP"
BASE COUNT         2616 a         1593 c         1721 g         2892 t
ORIGIN      
        1 atgaaaaaaa cagaccttgt gatatttctt accccgcgta tcattacaga acaaatcggt
       61 aaaaaaattg cagaagaaga aaaagttcgt acaggaactg acaaaggtac gcaatgaaaa
      121 aaagttcttg ttctggtttt acgattattg aatttcttct tgcttttgta attattgcaa
      181 ttgccttgat aggaacatcc gctttttatt ttgccagtag tagaaatctt catcaatctg
      241 atattacccg gcttgcaaca tggaaagcga ttgaaaaaat ggaaatgata aaatccactg
      301 attattcatt gattcaaaat gaaactgaaa atatcattgt tggaaacgtg caagtggaaa
      361 gaattacaac tgttagcgaa gatacgacat tgccattaaa gactgtcgaa gtgaaaatcc
      421 agaaaacaga cggcacaaaa attgttggca tttcaacaat gattgtacga ccatgaaaat
      481 tcttaaaata aaaggtcttg ggcttattga attgatgatt gttcttgtgc ttggtgttct
      541 tgcgatattt gcggcaagca gtattgttat agaatcatac agagagtgga aacgaagcaa
      601 tgaagttgcg tccttacagg ttgattttga cctggcatgc catatgataa aaagtattat
      661 tgaagaggcg gatacagttc aggtcagcga caatggaaca agtattactg caggttatca
      721 atcatactgg attcagcaat tttatcaaga cccacaaaat ccgtctgtgc tcgtctggaa
      781 aaatatgaaa acaaatgaat cacaaaaagt aattacaact ttaaaaaaca tcagttttga
      841 tgttcaggat gaccttgtag tggtgaatat tgaggtcggt aatgggacaa agtcaattgc
      901 aggttctttt tctgtttata tgaggaattc ataaatgaaa aaaggatata ttttacctat
      961 ggcaataatt tttgtcgtaa tatcgctgat tatgggaatg agtattttgt atttaggtgg
     1021 cacagcacag atatcttcaa tgaaaagata tcataaagaa aaggcatttt atattgcaga
     1081 agcaggcgca aactgggctt ttgcaaataa aagggcaaac gaatcatggc atccaggagc
     1141 aggtacggtt agttttgacg gtggaacatt tcagataaat gagattgttt caggagaaac
     1201 aattattttt gaatccacag gcaggtacgg taatcaggaa tcaaccgttt cgattgtaac
     1261 ttctcgcact ggtggttcag gcggcggtac tggtggttct tttggaaagg ggttatttgg
     1321 aagccaatca gtgactcttt atgaaaatgc ctgggttgat agttatgatt cgcgtctggg
     1381 tccttatgga ccttcaaata gaggaaatca cggtgatacg ggaagcaaga atacagtaac
     1441 tctctataat aacgcacata ttcatggaac tgcaatggtg gaagatggac cgcagtattt
     1501 ataccttggt cataatgcta ctgtaactga cagcgattta tatcacagtt ttgaagatcc
     1561 tcttaacaat ttacctgctg ttgttgtacc ttcggattta atgaatttgt tatatcctgt
     1621 gcagggtgac ccacgaatta ccggtaatta cacaatttct aatggcagat taactgtgaa
     1681 caataaccga accgttacca tttctggcgg tgatttcaaa ttcaaaagta ttacaatgaa
     1741 taataattcc attatgtata ttacaggcaa cacaagatta tacattgaat cgtccttgac
     1801 cctttcaaat aatacgcagg tgattattca aaacaacagt gttgttatct ggtatttagg
     1861 taaccagggg acgcctttta cccctaccct cgcaaataat tctgttatta ataacacttc
     1921 acagacaaca ggaaatttga ggatttatgt tgcaagttcc acaaatatga cgcttgctaa
     1981 taatgcgcag gcatttaatg gtgtacttta tgctccaact tcaagtatta ctcttgcaaa
     2041 taatagccag ttttttggtg gtattgtttc caatgtcctt actattgtaa ataatgcagt
     2101 tgttcactat gatgtggcat tgcgtgagat aaattttcct gaagaccccg ggttgggtac
     2161 tggtccaggc acacgaatga ttgtacgctg gacaaagcct gactggtcag ccagattgca
     2221 ataatcctta taaataataa cagggacagt ttatgtgtga gttgcttttt ttttcgccta
     2281 ctgatagaaa atacagaaga gaaaacagaa aatggcaggg aattccagga attacaatga
     2341 cttctgatgg cactctgtgg gcagtatggt acagtggtgg agatggtgaa ggacctgaaa
     2401 attacgtttt ggttgtcaat agcaaggata agggcataaa ctggagcgaa cctatcattg
     2461 tgattgatcc acctgatgac atacgcgcat ttgatccctg tttgtggaca gacccttatg
     2521 gtaaattatg gttattctgg gcaatgagta aaaactggtg ggacggaaaa gcaggcgtct
     2581 gggcaattac tggcgagcag gttggtgatg gatgtgtatg gagtaaaccg cgaagaattg
     2641 cagatggtat tatgatgaat aaaccagcgg tactttcaac cggtgaatgg ctttttccaa
     2701 tcgcagtctg gaacagagaa ccatttttac ctgaaatgga caatctacga ttttcaaatg
     2761 tttatatatc aaaggacaat ggaaaaacaa tcacatttct tggaagcgct gatgttcctg
     2821 agcgaatgtg tgatgaacac atgatagtgg aattgaaaac tggtgttctg cggatgcttg
     2881 tcaggacatc ctatgggata ggtgaaagtt tttcttatga caggggaaag acatggataa
     2941 aaggttgttc ttctaatatt gctggacctg gttcgagatt ccatatcaga agattaaatt
     3001 ctggaaattt gcttttgata aatcactatg ggttcagtga gaaaaatcgt tcccatctga
     3061 ctgccatgct ttctgaagat gatggaaaga catggcctta taagcttctt cttgatgaaa
     3121 gggataaggt ttcataccct gatgctattg aaactaaaaa cggggaaatt tttatcatat
     3181 acgattacga ccggtatgga aagaaagaaa tcttgatggc ggttgttaca gagcaggata
     3241 ttaaagcagg ggaaattatc acacctgatt caaaattgaa aatgcctgtt gacgacggat
     3301 aagtcatgga aaacatcctt tcttttcttg caaatttcat tgtgactatt attgaaaaaa
     3361 ctggttatct gggtgttttt attctaatga catttgaatc agccctgatt ccaattccgt
     3421 cagaaattac aatgcctttt gcaggatacc ttgtttcaat caacaagatg aatattcata
     3481 ttctttcttt tattggagca ctggcaaatc ttgttggttc aatcgccgca tatctgcttg
     3541 gtttctggct tgaagaccgt ctgatacggc aatggataaa aagacgaggt aaattccttt
     3601 taatttcaga agatgactat gacagggcag aaagatggtt cagaagatat ggagaccccg
     3661 ttgttttctt ctcacgaata acgcctggta tcaggacatt tatttcacta cctgctggag
     3721 ttgcaaaaac aaattttggt aaattctgcg tatttacctt tatcgggtct cttatctggt
     3781 cttattttct ttcctatatt ggttttttct ttggtaaaaa ctggtcacgc attgaaattt
     3841 attacagaaa atatgaatat ctaattcttt ttctaattat tttgcttatt tcgtggttta
     3901 ttttcagcaa ggtaaaaaag aaaaaaactt gattattccc acccttcttt tccttttaat
     3961 ggtacaaact gacatccgcc gtgattttct tgtatcagtt tccctttttt ctttatggcg
     4021 cgaattaagt cctgtgagta aagtgttcca acaggtatga taattcttcc accttcatcg
     4081 agttgttcaa ttaaagattg cgggatatca ggtgcgcctg ctgtgacaat gatgatttta
     4141 taaggagcgt attgttccca tccaagtgtt ccatcaccga ccatgatgtt tgcattgtag
     4201 ccaagttttt caaggatttt ttttgccctt tctgcaagtt cagggatttt ttcaactgaa
     4261 aataccctgc aaccaagttc acacaatatt gctgtttgat atcctgaacc agtacctatt
     4321 tcaagtaccc tatcatcttt ttcaggattg gtaagttctg tcataattgc aaccatatag
     4381 ggttgactga ttgtttggct ataatcaatc ggcaaagggc aatcgtcata agcagatgat
     4441 ttgaactggt tggaaacaaa ttcttcgcgc ggaactttaa gaaaagcaga aagtacccgt
     4501 tcatttttta ttcctcttgc ccggatttga tgttccacca tgaattttct taatctttca
     4561 taatctggtt tttctgccat gatacacgca tattttcaaa agcatcaaaa aaaactttat
     4621 agtatccctg ataggtttta ttttgctttt ttcgctctgg taaattgtgc ttacgggaac
     4681 tgaactgatt tttactcctt tccagcaggc gcgtaaaagt atttctgttt ctgtttcaaa
     4741 gtggtttgtg ataatcagca tgttttctac cacttttttc ctgattgctc tgaatccatt
     4801 ttgagaatcc ggtatccatt ggaaggcaag aattgaaatc aaaattgaca ttgaaaggtt
     4861 tgttaatctt ctgattgggg gcatgtcgct accaatgatt tttcttttac cgacgataat
     4921 cccggccttt ttattttttt taattgcttc aatgaaaacg tgtaagtcag aaacagggtg
     4981 ttgaccatcg ccatccattg caatgataac gtcgtatggg agattttgaa aatgttgaaa
     5041 acctgttttt aatgccgcac cttttccttt gcatactgga tgggttatta caatagcacc
     5101 gtgctgtttt gcaatatttc cagtattatc gtttgagcca tcatcaacaa cacagacata
     5161 atcaacaatt tcaaggatgt tatccaccag tttgccaatg gttctggctt cgttgtgtgc
     5221 tggaatcaat accgcaatac tctctttatc cattttgaat tcctaatcaa taattttact
     5281 ctggatgcaa tgctcaagat attctttaat tcttacatac ttctctttaa gatacaactt
     5341 gttgtttaat attctttctg cctcatttct ggattcattg agaatttcga tgtctgtgac
     5401 gatgttacct atttttaagg gtagaacccc gtgttgtctt acgccaagca ggtcgcccgg
     5461 gcctcttaga gaaaggtcta tttctgccag cttcagtcca tcttcaattt caaggaatga
     5521 ttcaagtcta ctaacggatg cagggtcatc agtatgatga atcaggatac agtacccaat
     5581 ttcaccgctg cggcctaccc tgccccgcat ctggtgtaac tgagacaatc caaatttttc
     5641 agcctcatct atcactataa aatttgcgtc aggaacatca attccaacct caatgacagt
     5701 cgtagaaaca atgatacgta tctttttttc tctgaaccgt tcaatgattt tagatttttc
     5761 atgtcctggt agttttccat gaagcaatgc gacatactgc ggttcaacaa tttgagatat
     5821 tttattaaaa tgatagacga ccgaagattc ttctgaacct gtaatggttg gtgcaacaaa
     5881 atacccctgt ttttcatcgt atacctgcct ttttataaat tcgtagacct tttcagtttc
     5941 ttcatttttg aaaagataac tggtaattct tctgcttcct tttggtaatt gtccgattgt
     6001 tgacatgtcg acattgccat aaaaagcaag agccacactt cttggaattg gggttgcgct
     6061 cattgcaaga tagtgaaccg tgctgctttt tttctgtagt aaatttctct gttcaacccc
     6121 aaatttgtgt tgttcgtcca caacaatcgc cttaagttct ggatacacaa ttttttcatt
     6181 caacagtgcg tgcgttccga acagtacctg aatctggttt ttttctattt tttctcgtac
     6241 ttgttttttc attttttctg gaagatttcc ggtcaatagt gcactttcaa ccccacatga
     6301 aagaaaaaat gggctccagt ttagatagtg ctgttctgca agaatttctg tgggcgcaag
     6361 aaacactccc tgaccaccat cttttgaaaa aagccagaga aaaaatattg caaccactgt
     6421 tttccttgag cccacttctc cttgctaaag acgattgata attctacctt cactgaggtc
     6481 tttcagtatt tctttcatcg cctttgtctg gtctgccgtg gtttgaaatg gaagcatttt
     6541 tgcgaagtta tgaactgatg aactttcaaa tttgttttgc gaaatatttt cgatatgtcc
     6601 tgtggttatg atttttttat aaatgaggcc gatctgtagc ctgaaaaatt catcaaagat
     6661 caggtgtcgg cgtgcccttt caagatttat ttgattttcc gggaaatgta tgtttatgag
     6721 ggcgtgtttc atacttgaca gtttgaggcg acttctgtca tcaagtggaa ggatatctgg
     6781 tggaaattgc tgcatatttt ccagagcata tcggatacat tttcttaaaa aattttgcgt
     6841 taaacctgaa acgaccgaat agacgggaag gatgtaatca agctttcttt ttgtttcaag
     6901 aatttcaaac agtggattat tgatttgaag gttttctttg tagatgtcta ttcttccata
     6961 cacaaacact tttgttcctg gtttcaggat gtttaagata taattttgat tgaagaaaat
     7021 taaaaaaatc acgccggttc catcggatat cgcaattttc acgatggaaa gtctgttgat
     7081 tcttttttct tccactgcca taacatttcc tttcaacgaa acagtttctc cagggattac
     7141 ttcgctgatt ttcttgatct gtctcctatc aaggtattta cgcgggaaat gaaacagcag
     7201 gtctttgatt gtggtgatgc ccattttgta aaaatattct gctctccgga gacccaaccc
     7261 tggaatcatc ttaatagaac tttctggtag caacatgatc accgaagtgg caattccatc
     7321 tgttcctgtg acagggaaat aagtttataa aattttagta ccctcaaaag atcacagata
     7381 ggagaagaat agacctttct cgcttcgatt ttattaataa ccccggtgag aattaccggg
     7441 atggtgataa gatgattaat tcctgtactc tgaagttttt ccagcacttt ctgttttaaa
     7501 tcaatatgcg agggtaattg cgggatgacc aaaatttttg cgaaactttc ccctttgaac
     7561 cattcggaaa actttttttc tttgttttct tttgcaaatt caaggatttc cgggaatgtt
     7621 tcgagaatcc ttgttgtaat tttgcatgta tgccaggcga tgggtttaat aatggcggtg
     7681 gggatgtatt ccacaccagt ttcatcaaga atgaaaccta tgtctgaatt gtcttcggtt
     7741 tttgcctttt taacaagtac aagatttcca cttctcgcgg taaaatattt tctctgctga
     7801 aagaattctt ctaccagttc aagaatcact tcactcataa tcagggaata aagtaaagtg
     7861 aaaggaaaaa attaccttta tacagaattg caataatggc gcctattgct aaataaggac
     7921 caaatggaac ataatcgtcc cattttttct tttttgaata tataagagca agacttatta
     7981 cagttccaag aattgaacca aagaaaatgg aaagaaacac tcctttccat ccaagaaacg
     8041 ctccgattgc cgccagaagt ttaagatcac cgcctcccat ggcttctttt ttccatatta
     8101 tttttccgat tactgcaaga aatgctacaa gcaatccccc tattaatatt ccagcaagac
     8161 tgtaagatgc ccgtgttacc atagaccaac ttccattttg aggtaaaaag aaaccttcac
     8221 ataataaagc gcctatcaat cccagcggta ttaaaggcag tacaatgaca tctggtacaa
     8281 gaaatgtttc aaaatcaata ccgctgatga taacaagacc aaacgcaaaa ataaaataaa
     8341 acaggaaaac gtatgggaga tatgccaccc agaatttttg ataaagcaac agtgtcaaaa
     8401 gtcccatagc gagttcgatg attggatacc gtggagaaat cgttttttta cagtatcggc
     8461 atctacccca caggaggaga taactcagga ttggaatatt gcaataccat ggtattgaac
     8521 ggcgacattt aggacaaaag gacccaggag aaacgattga tttgtttctt ggtaatctgt
     8581 atatgcaaac atttgcaaaa cttccaaaac atgcaccgat aaaaaaaata agaaattctt
     8641 tcatggtatt aaaagtttct gaccagttgt aacaatatca gttttcatgt tatttgcttc
     8701 ttttattttt ttcatcgttg taccatactt ttttgcgata gaagaaagtg tttcgccctt
     8761 tttgacaata tgataagttt tttcagcaga ttcctttgtt ggtggagggt tttcaatttt
     8821 tc
//
LOCUS       DAORYU010000031         8792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_5834_length_8792_cov_3.906146, whole genome shotgun sequence.
ACCESSION   DAORYU010000031 DAORYU010000000
VERSION     DAORYU010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8792
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5834_length_8792_cov_3.906146"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="PLW07_01875"
     CDS             <1..850
                     /locus_tag="PLW07_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49303.1"
                     /translation="SSTKDKFIIQQIKDILKNFKQYLKEDTMVDSTCKFVVRNLCQRR
                     YFFTKFISITETCPLLFQETEILEIIPFAADRKYVDCEIQCVSSDKISMTDPPTMIFQ
                     KENRYFARLKKGIAFSPDRWRKSEGISLIFPWHDITTNQIKVIRNYSTEGEFAISSII
                     FEKLKPGMKIEVYFSTRAGKFESVSPESSEPGPGGSMVFQPANEKYEVKIKFKPTADL
                     LAYYPKIRIVVEEKTESRMDEDASEISFETDRTEFQLRFDTDLKVHSVFRTSEIVYEI
                     DEKIDI"
     gene            831..3101
                     /locus_tag="PLW07_01880"
     CDS             831..3101
                     /locus_tag="PLW07_01880"
                     /inference="COORDINATES: protein motif:HMM:NF027900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HOL49304.1"
                     /translation="MKKLIFSGIVLVFGGLTLLQAETLFDFENDIQNWNSMYPQQTLK
                     ISKNFVSSGMNSLAVDVSGMKESWIRVDADFDLTHSDVLSLDIYIPSKTKNYVSFICY
                     IKDKNWTWFQTGLYQINLNEKKKIIIDISDTSSEWQPATGLACWDGYTKKNIREFGIK
                     FFFYLPFSGTIYIDNIQALPKQAEQQFFLFNFETNAETIPQYDKFEVLFEVPVVFDNP
                     FDPEEIDIAGTFHSPSGKEISVPAFFDRDYIRCLEEDGEKLYPYGKHRWCIRFAPEEI
                     GDYRYEIVINFAGKTTRFDCGWFKCVSGTNPGFIRWDNQDLQCLSFSNGKFFYPIGHV
                     LRSPDDLRQPYKYEFIPDKGMGTFAYDGYFKKMKDNLENYIRMWMGAWWVGIEWTPAY
                     AQHYEGIGRYSLENSWKLDYVLDIAKENGIYIVLTLINHGQFSIRPDAEWWDNPYNVI
                     NGGFLNSPDEFFYSSEAEKWFLKRLRYIVARWGYSTQIAFWEMWNEVDLTGYYDSRNI
                     RQWHERFISLLRKMDPWKHPVTSHICRPTDDPMVWIVKELETLVSNAYSQTVVDTMRE
                     YYFRRKPFEKPMQIHEFGVGRNSRELENNLHAGIWTSSLSPMLGTALFWWWPFIDKKD
                     LYFHYRALARFLDGLDRRGKNFQISTAKIISASGSISTKAGVTGMQNMTSAYLWVYDN
                     AVFNSRNPSEKEQSIDDVKLVVEIFDPGIYRIEFWDTYKGERFSDFEKRRENGECEIS
                     LPSFTKDIAVKIEKKS"
     gene            3098..5125
                     /locus_tag="PLW07_01885"
     CDS             3098..5125
                     /locus_tag="PLW07_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49305.1"
                     /translation="MKKFIILIILACFVNNCSGYTLRAKLKIKQPQLKQFQQIAEVDL
                     PLPEKGYVYVADTDGLKVPCVRIKNGSQQRDTVYFRTNKNNAYYVYWLSEDKKQDTGI
                     FEVPEGRVILDDFINPSARTSGFWYWGSHVCLSGKFSHSGRSLQKINSHYTAVVPGEK
                     TKSGDIFYQYVFIDKNQPASEIMLEIQTQKRKSVYFSWGSDIIRWKNLNKVRMGDLPE
                     TGRWIQLLIPVDKIDKNVEITGIGFYHADGRVFWDYTTIGNPPLDTEITLWNKENKKT
                     SAFFDKEVSRPFTFSGKEFNLVLLNASASSGADTFEWTIDNEIQSGAGDKILKRVDYK
                     DRIFVKLFATNTTLKQSDIFSETILFQKRSPEQLNFALEILPHKNLVGQKETFFVPVR
                     MGSLMSGPVPVELSIDGRKELFELLPGKENAEIINVFFSPEKPEIHEIELKLDDLTIA
                     QKKLRFVPVDQIGEEYLDGPYLKDENNNRLVAFVPDYRCSDVRTDTQISQLTVIGDIP
                     ENSVNRMKGKLSKNINIEWLRYPEEKHYHAIGDLLWLKSRIEKGDFDTVILFPSIETL
                     LRRTPVDQYIACLDAEMWFLSKRAKRIICVTPFPSAPIPGIFKIYADATVALCAKRNI
                     ICLNLYDLYTSLPGWTELFSPSPGIYRNLPMEKGTEILIGAIVKILGTSQE"
     gene            5129..6295
                     /locus_tag="PLW07_01890"
     CDS             5129..6295
                     /locus_tag="PLW07_01890"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOL49306.1"
                     /translation="MKIQRDVFEGVDEKGRPVFPGFISYVDNEKPILIHRYGRLDYSD
                     AYDDFCDCFSHDNGKSWTQPQLRLKGYQFSGGTYIRYAENACLFDRTRNKLFTFASKR
                     TYTGNKIDVDGPSWVVYDVFDWQEQKWKGEQEISLHIPGGVFISFCFPVALNKDILLI
                     PAITRMIDKNRNIVHYKGCWAPANRSFLIKAKILTNCEIEFSTGQPAEIDLEMSSRGL
                     GENTIAVLKSGTIAMVCRGDNSMFPERPGYKWVCFSSDQGENWSLPQPLICDDGTFFE
                     SGSNGSALFRAEKNGKLYWIGNLCITGEKAKGNWPRYPLVIAEINEEPFCIKKSTITV
                     VDTRQNRETETVQLSNFRFYQDRENGDIVVFLTRFGENDTNWKGANYYRYRITL"
     gene            6307..7380
                     /locus_tag="PLW07_01895"
     CDS             6307..7380
                     /locus_tag="PLW07_01895"
                     /inference="COORDINATES: protein motif:HMM:NF013430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HOL49307.1"
                     /translation="MIVGVLREIKTEEYRVGLAPMGVRELVKQGHKVLVEKNAGIGSS
                     FSDTLYSECGAEIVSRDSIFESAELIVKVKEPQDSEISMMKPGQIVFTFFHFASNHEM
                     TRALANKKVTCIAYELIEENGTRPILKPMSEVAGKLSIQQGMKYLEKEYGGKGVLLMG
                     TETVPPGSVVVLGGGVVGSSAVGVALSLNAKVTIIEKSPEKREILKRRFPDCDVMDSK
                     ENIITERCQKADIIVGAVLLPGHRPPILIKKSDLESFEKGTVLVDVSIDEGGVFETSR
                     PTTHKEPVFIDSGIVHYCVPNMPGIVPKTSTIALNFATMPYLKMLANMGESALEENSA
                     LKSGCAMLRGEIVHPAIKEIVNS"
     gene            complement(7383..8759)
                     /locus_tag="PLW07_01900"
     CDS             complement(7383..8759)
                     /locus_tag="PLW07_01900"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOL49308.1"
                     /translation="MNKILTEEKNLVKSIKAANSFLNKNSEKLLLNNVGRIIKNIPSP
                     PKTTKKWFQKSYVLGPEQNDFLAGKGVFYITEGRKLFLDATAGHYQMTWGYNHPTLTK
                     CLDEAIALEIPWDCHSNIPGFFVKKLSEKLVEICNPERNYASLLNDENCLNTVLLGIA
                     TGSVACSTAMKLTLNYYKSQKPRGKKIVFVSLKGNYHGTDFLMQRLRKMWDWMFAKEI
                     IFEIVEPNDLNGLDRVFKKHKENIAGFFFEPVMMNREAILLTREFIQKARKLTDSVNA
                     LMIVDEIQTCCWYPEIFMYKQFDITPDVLILGKGLTAGFHPLSAVVYKRKLDSLKQYD
                     AISTNGNAPLPSLMAMCCLDLIEKNIKKIEVLGSYYEDCLKNLAREFPHLIVSIQGKG
                     FLSGLKLNNQQTALKFHQKCIEKGIWLRVHAYYEGHSTALTKFALSVSKDVLDYTVNI
                     FRKILKII"
BASE COUNT         2776 a         1456 c         1867 g         2693 t
ORIGIN      
        1 ttcatctacg aaggataaat ttatcataca acagatcaag gatattttga aaaattttaa
       61 gcagtatcta aaagaggata cgatggttga tagcacctgc aaatttgttg tccgcaatct
      121 atgtcagaga agatactttt tcaccaaatt tatttctatt acggaaactt gtcctttact
      181 ttttcaggaa actgaaattc tcgagattat tccttttgct gcggacagaa aatacgttga
      241 ctgtgagata caatgtgttt ccagtgataa aatttcaatg acagatcctc ctacgatgat
      301 atttcaaaaa gaaaacaggt attttgcaag gttaaaaaaa gggattgcat tcagtcctga
      361 cagatggaga aaatcagagg gcatttcact gatttttccc tggcatgaca ttaccacaaa
      421 tcaaatcaaa gttattcgca attattccac tgaaggagaa tttgcaatat cttcaataat
      481 ttttgaaaaa ttgaaaccag gaatgaaaat tgaggtatat ttttccacaa gggcaggaaa
      541 attcgaatct gtttcaccag aatcatctga gccagggcct ggcggttcta tggtttttca
      601 acctgcaaat gaaaaatatg aagtgaaaat aaaattcaag ccgactgctg atttgcttgc
      661 gtattatcca aaaataagaa ttgttgtgga agaaaaaaca gaatcaagaa tggatgaaga
      721 tgcttctgaa atttcttttg aaacagatag aacagaattt caacttagat ttgacactga
      781 tttgaaagta cattccgttt tcaggacaag tgagattgtc tatgaaattg atgaaaaaat
      841 tgatatttag cgggatcgtt ctggtgtttg gcggattaac actgctacaa gcagagacac
      901 tttttgactt tgaaaatgac atacagaact ggaactcgat gtatccgcaa caaacactga
      961 aaatttcaaa gaattttgtt tcaagcggga tgaattcact ggctgttgat gtatcgggta
     1021 tgaaagaatc ctggataagg gttgacgctg attttgacct gacacactct gacgtgctat
     1081 ctctggatat ctacatacca tcaaaaacca aaaactatgt ttcttttatt tgttacatca
     1141 aagacaagaa ctggacatgg tttcaaacag ggctttatca gattaacctg aacgagaaga
     1201 aaaaaatcat aattgatatc tctgatacaa gttccgagtg gcagccagca acaggtcttg
     1261 cctgctggga tggatacaca aagaagaata ttcgcgaatt tggaataaaa tttttcttct
     1321 atttaccgtt ctcgggtacc atttacattg ataatatcca ggcacttcca aaacaggcag
     1381 aacagcaatt tttccttttt aattttgaaa caaatgcaga gacaattccg cagtatgaca
     1441 agtttgaggt attgtttgaa gttcctgttg tttttgataa tccctttgac ccggaagaaa
     1501 ttgatattgc agggaccttt cattcaccct caggtaaaga gatttcagtt cctgcctttt
     1561 ttgacaggga ctatattaga tgtcttgagg aagatggtga aaaattgtat ccatacggga
     1621 aacatcgctg gtgtataaga tttgcgcctg aagaaattgg ggattacaga tatgaaattg
     1681 ttataaattt tgctggaaaa acaacaaggt ttgactgcgg ttggtttaag tgtgtttctg
     1741 gtacgaatcc cggatttatc agatgggata accaggattt gcaatgtctt tctttttcaa
     1801 atggcaagtt tttttacccc attggacatg tccttcgttc tcctgatgat ttacgacagc
     1861 cctataaata tgaattcatc cctgataaag gaatgggtac gtttgcgtat gatggttatt
     1921 tcaaaaaaat gaaggataac cttgaaaatt atattagaat gtggatgggc gcatggtggg
     1981 tgggtattga gtggactcct gcttatgcac agcattatga gggtattggt cgttacagtt
     2041 tagaaaattc atggaaactc gattatgttc tggatatagc gaaggaaaac ggcatataca
     2101 ttgttctaac acttattaac catggacagt tcagtatcag gccagatgct gaatggtggg
     2161 acaatccgta taatgttatc aatggcggct tcttgaattc ccctgatgag tttttttata
     2221 gtagtgaggc ggaaaaatgg tttctgaaga gattaagata tattgtggcg cgctggggat
     2281 attcaaccca aattgctttc tgggaaatgt ggaatgaggt tgaccttact ggatattatg
     2341 attccagaaa tatcagacaa tggcatgagc ggtttatttc tcttttaaga aaaatggacc
     2401 cgtggaaaca tcccgttacc agtcatattt gcagacccac tgatgacccg atggtctgga
     2461 tagtgaaaga acttgaaacg cttgtcagca atgcgtacag tcagacagta gttgatacta
     2521 tgcgggagta ttatttcagg agaaaaccct ttgaaaaacc aatgcaaatt catgaattcg
     2581 gtgtgggaag aaatagtaga gaacttgaaa ataatcttca tgcaggaata tggacatcat
     2641 cgttatcacc aatgctcggg acagcgctat tctggtggtg gccctttatt gataaaaaag
     2701 acctgtactt tcattacagg gcacttgcca ggtttcttga cggtctggat agaagaggca
     2761 aaaatttcca gatttctaca gcaaaaatta tttctgcatc tggaagtatt tctacaaaag
     2821 ccggtgttac tggcatgcaa aatatgactt ctgcatatct gtgggtctat gataacgcag
     2881 ttttcaattc aagaaatccg tcagaaaaag aacaatcaat tgacgacgtg aaacttgtcg
     2941 ttgaaatttt tgatcctgga atctatagaa tagaattctg ggatacttat aaaggtgaaa
     3001 gattttctga ttttgaaaag agaagggaga atggtgaatg cgaaatttca cttccatctt
     3061 ttaccaaaga catagcagta aaaatagaga aaaaatcatg aaaaaattta ttatattgat
     3121 tattctcgcc tgttttgtga ataattgttc tggctataca ttgcgagcaa aacttaaaat
     3181 taagcagcca cagctcaaac agtttcagca aatagctgag gttgatctgc ctttgcctga
     3241 aaaagggtat gtttatgtgg cagatacaga tggtctgaag gtaccgtgcg tcagaataaa
     3301 aaacggttcg caacaaagag atactgtata tttcaggaca aacaaaaata atgcttacta
     3361 tgtatactgg ctttcagagg ataaaaaaca ggacaccggg atttttgaag ttccagaggg
     3421 tagagtgata cttgatgatt ttattaaccc ctctgcaaga acctcaggat tctggtactg
     3481 gggttctcat gtttgtctga gcggaaagtt ttcacactcg ggaagaagtt tacagaagat
     3541 taattcacat tatacagcgg ttgtgcctgg agagaaaaca aagtcagggg atatttttta
     3601 tcagtatgtt tttattgata aaaatcagcc agcatctgaa attatgcttg aaatacagac
     3661 acagaagagg aagtctgttt atttttcatg gggttctgac ataatcaggt ggaagaatct
     3721 gaataaagtg cgcatgggtg atcttcctga aactggcagg tggatacagc ttttgatacc
     3781 ggtggacaaa attgataaaa atgttgagat caccggcatc ggtttttatc atgctgatgg
     3841 cagggtattc tgggattata caaccattgg aaatccacct cttgatacag aaattactct
     3901 atggaacaag gaaaataaga aaacaagtgc cttttttgat aaggaggttt ctagaccttt
     3961 tacgttttca ggaaaagaat tcaatcttgt tttgttgaat gcttcagcgt cttctggcgc
     4021 tgataccttt gaatggacga tagacaatga aattcaatca ggtgcaggag ataaaatatt
     4081 aaaaagggtt gattacaagg atagaatatt tgttaaactt tttgccacca acacaacctt
     4141 gaagcaatca gatatttttt ctgaaacaat cctgttccaa aaaaggtctc ctgagcaatt
     4201 gaattttgct cttgaaattc ttcctcataa aaatctggtt ggtcagaaag aaacattttt
     4261 tgtgcctgtt cgtatggggt ctttaatgtc aggacctgtt cctgttgaac tttccataga
     4321 tgggcgtaaa gagttgtttg aactgttgcc tggaaaggaa aatgctgaaa ttatcaatgt
     4381 atttttttct ccagaaaaac cagaaattca tgaaatagaa ttgaaacttg atgaccttac
     4441 cattgctcag aaaaaattgc gttttgttcc tgttgaccag attggtgagg aatatcttga
     4501 tggaccttat ttgaaggacg aaaacaataa tcggttagtg gcatttgttc ccgattacag
     4561 atgctctgac gtacgaactg atacacagat cagtcagtta acagtcatag gggatatacc
     4621 tgaaaattct gtcaacagga tgaaggggaa gttatcaaag aacattaaca ttgagtggtt
     4681 gaggtatccc gaagaaaaac attatcacgc tattggtgac cttttatggt taaaatcccg
     4741 gatcgagaag ggagattttg ataccgtgat tttatttcct tcaatagaaa cccttttaag
     4801 aagaacgcca gttgatcagt atattgcttg tcttgacgca gagatgtggt ttttgagcaa
     4861 aagagcaaaa aggataatat gtgtaactcc ttttccttct gcgccaattc caggcatatt
     4921 taaaatttac gctgatgcaa cagttgctct ctgtgctaaa agaaatatca tctgtcttaa
     4981 tctttacgac ctttacacca gtttgccagg ctggacagaa ctattcagtc catcgcctgg
     5041 catatacaga aaccttccaa tggaaaaggg cacagaaatc cttattggtg ccattgtgaa
     5101 gatacttggt accagtcagg agtgaattat gaaaattcag agagatgttt ttgagggcgt
     5161 tgatgaaaaa ggtagacctg tatttccagg ttttatcagt tatgttgata atgaaaaacc
     5221 tatactaatt cacaggtatg gaagattgga ttattctgat gcctatgatg acttctgtga
     5281 ttgttttagt catgataacg ggaaaagctg gacacaaccg caattacgat taaaaggata
     5341 tcaatttagt ggtggtacct acattagata tgctgagaat gcttgtttgt ttgatagaac
     5401 aaggaataaa ctttttacat tcgcttcaaa aaggacttat actggcaata aaattgatgt
     5461 tgatgggcca tcttgggttg tctatgatgt ttttgactgg caagagcaga aatggaaagg
     5521 tgaacaagaa ataagtttgc acatacctgg aggagttttc atcagttttt gttttcctgt
     5581 cgcgcttaat aaagatatat tacttattcc tgcaattaca cggatgattg ataaaaaccg
     5641 gaatattgtt cattataagg gttgttgggc tccggcaaat cgttcgtttt tgattaaagc
     5701 gaaaattcta acgaattgtg agattgagtt ttcaactggt caacctgctg aaattgacct
     5761 tgaaatgagt tcacgcggac ttggtgaaaa taccatcgca gttttgaaaa gcggaacaat
     5821 cgcaatggta tgcaggggtg ataatagcat gtttcctgaa aggcccggtt ataaatgggt
     5881 ttgtttttcc agtgaccagg gagaaaactg gtctttgcca cagcccctga tttgcgatga
     5941 tggtacattt tttgagtcag gttcaaatgg ttctgcgctt ttcagagcgg aaaaaaatgg
     6001 taaactatac tggattggaa acctttgtat aacaggtgaa aaagcaaaag ggaactggcc
     6061 aagatacccg cttgtgattg ctgaaatcaa tgaagagccg ttttgtataa aaaaatcaac
     6121 aattactgtc gttgatacaa gacaaaacag agaaacggaa acggttcaat tatcaaattt
     6181 tcgattttat caggatagag aaaacggaga tatagttgtt tttctcacca gatttggtga
     6241 gaatgataca aactggaaag gtgcaaatta ttacagatac cgaataacat tatagaggag
     6301 gttgcaatga tagtaggagt tttgagagag ataaaaaccg aagagtatag ggttggtctg
     6361 gcgccaatgg gtgtaaggga actcgtgaaa cagggacata aggtccttgt tgaaaaaaat
     6421 gcgggaattg gttcatcatt ttctgacact ctttatagtg aatgtggggc tgaaattgtg
     6481 tccagggaca gtatttttga atccgcagaa ctgatagtga aggtgaaaga accccaggat
     6541 tcagaaatat caatgatgaa acccgggcag atcgtattca cattttttca ttttgcttca
     6601 aaccatgaaa tgacacgcgc tcttgcaaat aaaaaagtta cctgtattgc ttatgaactt
     6661 attgaggaaa acggtacaag accgattctc aaacccatga gtgaggttgc tggtaaatta
     6721 agtatacaac aggggatgaa atatcttgaa aaggaatatg gcggcaaagg agtacttctg
     6781 atgggaacag agactgttcc gccgggtagt gttgttgttc tgggtggggg agttgttggt
     6841 agttctgctg taggtgttgc attatctctt aatgcgaagg ttacaattat tgagaaaagt
     6901 ccagaaaaaa gagaaatttt aaagagaaga tttccagatt gtgatgtgat ggattcaaaa
     6961 gaaaacatta ttactgaacg ctgtcagaaa gcagacatca ttgttggcgc tgtgttgctt
     7021 ccagggcaca gaccaccaat tcttattaaa aaatcggacc tcgaaagttt tgagaaagga
     7081 actgtcctgg ttgatgtttc tattgatgag ggtggggttt ttgaaacaag tcgtccaacg
     7141 acccacaaag aaccggtttt tattgattca gggattgtgc attactgcgt accgaatatg
     7201 ccaggtatcg taccaaaaac gtccacgatt gcgctcaatt ttgcaacaat gccatatttg
     7261 aaaatgctgg caaatatggg tgagtctgca ctggaagaaa attcagcatt aaaatctggt
     7321 tgtgcaatgc tacggggtga gattgtacat cctgcaataa aagagattgt aaattcctga
     7381 aactaaatga tttttaaaat tttccggaat atgtttaccg tataatccag tacatctttt
     7441 gacactgaaa gagcaaattt tgttaaggca gttgaatggc cttcgtaata ggcgtgaacc
     7501 ctgagccaga ttcccttttc aatgcacttt tgatggaatt taagggctgt ttgctgattg
     7561 ttcagtttga gtccggataa gaaacctttg ccctgtatgg aaacaatcag gtgcggaaat
     7621 tcccgggcaa gatttttcaa gcaatcttca taataactgc caagcacttc aatctttttt
     7681 atattcttct ctattaaatc caggcaacac atagccataa gggaaggcag tggtgcattt
     7741 ccgtttgtac tgatggcatc atactgcttt agggaatcaa gtttcctttt ataaacaacc
     7801 gcagagagtg gatgaaaacc tgcggtaagt ccttttccga ggattaaaac gtcaggagta
     7861 atatcaaact gtttgtacat aaaaatttca ggataccagc agcatgtttg tatttcgtca
     7921 acaatcatga gcgcgttcac agagtcagta agttttcttg ctttctgaat aaactctctg
     7981 gtcagtaaaa tcgcttctct gttcatcata acgggttcaa agaaaaagcc agcgatattc
     8041 tccttatgtt ttttgaaaac cctatccaaa ccattcaggt catttggttc aactatttcg
     8101 aaaataatct ccttagcaaa catccagtcc cacattttcc tgagtctttg catcaaaaaa
     8161 tctgtaccgt gataattgcc tttcagcgaa acaaatacga tttttttacc gcggggtttt
     8221 tgactcttat agtaattcaa agtaagcttc attgctgttg aacatgcaac agatccggtt
     8281 gctataccaa gaagaacagt gttcaagcaa ttctcatcgt tcaataaact ggcatagttc
     8341 ctttctggat tacatatctc aaccagtttt tctgaaagtt ttttcacgaa aaaaccagga
     8401 atattgctgt gacaatccca tggaatttca agtgctattg cctcatcaag acactttgtc
     8461 aatgttgggt gattgtagcc ccatgtcatt tgatagtgtc ccgctgtagc atcaagaaac
     8521 agttttctcc cctcagtaat atagaaaaca cctttccctg caaggaaatc attttgttct
     8581 ggtccaagaa cataggattt ctggaaccat ttttttgtcg tttttggcgg agatggaata
     8641 tttttgataa tcctgccaac attgttgaga agcaattttt cgctgttttt gttaagaaaa
     8701 ctatttgccg ctttaatact ttttacaaga tttttttctt cggttaatat tttattcata
     8761 aatttccgtt tttatagcaa atatggtttt cc
//
LOCUS       DAORYU010000032         8664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_5935_length_8664_cov_4.562667, whole genome shotgun sequence.
ACCESSION   DAORYU010000032 DAORYU010000000
VERSION     DAORYU010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8664
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5935_length_8664_cov_4.562667"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="PLW07_01905"
     CDS             <1..290
                     /locus_tag="PLW07_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013006939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOL49309.1"
                     /translation="NGYRILEKNYRTKSGEIDIIARKNRSIVFIEVKTRSNDSFGSPV
                     EAVDRRKLAKLEIVANQYIQKKEIEDAAFRFEVISITKINNAWQCEIIPVD"
     gene            290..1807
                     /locus_tag="PLW07_01910"
     CDS             290..1807
                     /locus_tag="PLW07_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOL49310.1"
                     /translation="MLAKIHSVAVWGIEGYPVSVEIDISKGMPSVAIVGLPDQAVKES
                     RDRIKPAIKNSGFEFPQSKITINLAPADLKKEGSCFDLPVALGILAARQIISSDVASR
                     FHFIGELALDGSVKPVSGVLPMAMSVKQSGGKFVVPKANAIEASVVGIEAYGVESLKE
                     CVDFLTGKKEILPYNVDWDNILAVQNKYDIDFKEVKGQAVARRAIEVAVAGAHNLLVI
                     GPPGAGKSMIARRVPTIFPPLTIEEAIEITKIHSVAGMLAGGIVSTRPFRQPHHTISD
                     IAIIGGGTIPKPGEISLAHHGVLFLDELPEFHRDVLESLRQPLEDGKVSVSRARGRID
                     FPSRFFLIAAMNPCPCGWYGDSRRPCRCTVPQIIKYRKKISGPLLDRIDIQIEVSGLG
                     SKQLVSDSVSEDSESIRKRITRCRKIQQERFAGTGIFCNAHMNTKQIKQFCILDDECR
                     KFLENALEKMNLSARGYDKVVKVARTIADLEESEKISLSHIAEALQYRSFDRELW"
     gene            1816..2922
                     /locus_tag="PLW07_01915"
     CDS             1816..2922
                     /locus_tag="PLW07_01915"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOL49311.1"
                     /translation="MNKKVKPGKIVMELPPAGENARNSEGTFITLKSGRIVFAWSKFF
                     GSHSDFGEAVIAAKYSDDDGKTWSKDDRVLIEKEGTTNVMSPSFLRLQDGRIAIVYLR
                     KDGKKSCMPYFRTSDDELKTFSEPVSIAYEPGYYVVNNDRAIQTKCGRIVVPAALHRY
                     RGPSVLLPGEEPKTFLASPAIILFFLSDDNGKHWMESLTNYYKCFPSGHGLQEPGVIE
                     LNDRRLWSWTRPGTEGRQWESFSDDLGQTWTEPRPSQFVSPCSPMQIKRIPKTGHLFA
                     VWNDHSGIFKTEKPHPVSWGRTPLVCAISQDEGNTWQHHKLLEDSPEHGFCYPAIGFT
                     EEAVLISYNAGGITSKIPLDTQRIRRILLVDLYD"
     gene            2915..3121
                     /locus_tag="PLW07_01920"
     CDS             2915..3121
                     /locus_tag="PLW07_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49312.1"
                     /translation="MTEKRFIGTSGWNYKIWQDTFVLMNRYKQTQTGFVFSGKVHRSI
                     THIKRFIEVEEAWKTQRCFCIFQQ"
     gene            3182..7417
                     /locus_tag="PLW07_01925"
     CDS             3182..7417
                     /locus_tag="PLW07_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49313.1"
                     /translation="MKKLCLSFFMLFAVAFCYAGINITYKGLDNIHIQFDHQTSKQCT
                     VKLVCGETILFSQVLPQGGGSVSANIHHTGLSNGTYTYNIFEDEDVVSSVSETINGQV
                     CGTLLFDETINIPADVISINVPEGKTLDINADISEISFSSIDVVGNINLQNGITIQGV
                     TIYFGGQPVAIRNIKLTGSGEFYFKEASSGSSIENCDVSSVSVDPNTSIAVKNSKIGT
                     MLVVDSASLNAEESEISDLLCGGNIVLNRCSINGELFLINNAQNFTATDTCFLNRVNI
                     EGGSPQFSRCEFLNQLRFYGRNQANFDNCVFGNSVSFVDFGVFDNPKWSDNSGMQPIF
                     SHCSFVGQTGPYFEYTVPSLPVDLGGSNYYGDKKPDLDWFSTPIVHNELPNYPQGGRF
                     LLRGVPVNKQHFRIEQWSTSGPNLTCNKILPDIWANDYIVGQNTIPWPNYQNTSRQKV
                     LIKGRETLLSVDVATNYPLVQGVKFKAVFDGQEIAPVNPSIVLKRDISDSSNDVFYGN
                     TTINFILPPTENDIVNFTVIMDTTNISGFDDAAGKAEKVLFGGTLSFAPSYARQLNIL
                     VQPVQLFITGYTRKIPDASSVASALKNLIPAMLPIDRKDLHIWTAPVTTFYGGIFSMF
                     STTALLNRIANELALGQGLVNTTASVGDWLSGRETAMIDFIVAVMPKGSMGEGVSGVS
                     LALRRGIIFVDESAPDAALHEMGHGIGLYTGTEQYDQYPPAGLSLERMTAFLNDDRVS
                     INGFRNRFLHFPGTIHSWYQEKYWYDIMGSSTTLIWPNWTTFSAFAYYFNETLGNENK
                     TVKFLTKAVPSGNKRIFVSATTEKTNFNGGYKIKEGTISAFDITDFSTGKINVPVYWD
                     DDNIAVNDYIIKCFDSADNQIFNQPFTVVSPYPGYFGWVNWPEESSWCGTFDIPENTH
                     LIAIYPRPGWWNNFQDTPILEVNGVDYEPIKITGLKPGQQLGESVEIRWTQDTSKQSS
                     SMRYLVMYSEDGGNSWLPAGIPVEGNKVVLPTDFLPASEDIIFKVIGSNGLGTVEDEV
                     GGLKLENRAPKAVIVTPKEGWVGEVGTKWVFKGYGEDVEDGVIPQGQWSSSIDGEIDA
                     SKEVELSQGIHTITFTVTDSGGLTGSTSITVEVKQMQDIDIGLNEDSLQLFIEGKDPL
                     NTAPLVYLKENGVHRAILKIQNTGTDTIFTGAIYITKPGEAEQLLVEKTFTAGAFEEV
                     VISETFLVVAPGQYEIRGQIKDITPQDTEEGNNEEVWIYSTKPESPVILVAPEDLDFG
                     DAKARKQGQILVSNYGKLSLRITSMLISGADANQFGVDDTMLQQDIPEGNNVFIPVYF
                     NPVSTGTKQALLTITSNDINHSTIQIQLRGNCIGILGDISGDSSVDISDVILCLRQAI
                     GLDEPEIDSADMNEDGMIDISDVILVLRKAIGL"
     gene            7552..8559
                     /locus_tag="PLW07_01930"
     CDS             7552..8559
                     /locus_tag="PLW07_01930"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOL49314.1"
                     /translation="MKKVLCLAVAGLFLCVVGVFAQEGYLRITSNPESASVEIAGKVI
                     GKTPLLTVLKPGKYTYKVSLAGYETVSGTAEVVDYEVTNVNLVLVKQVQKAPAAKKVE
                     PAKGGLTIITDWQDVTVYLNGHKTDKVPPVTIKNVPAGINKVILVSGDYADSFHILVQ
                     PGKTSVLKKNFEDDKKKYEAQMTTVDTTVVETPVEVKKSMLPAKVILRLGTIASTDSK
                     KQDTTILGESDEIEVSFQYKKTGTDEWNTKTLQSGTKVEDSFETEKGTYDIQIIATHY
                     RVPTGLLNVLLTKKEKVREYKETIKKEILPDVQYTYTITYDGKSFGYKLEEAKLNTPV
                     K"
     gene            8586..>8664
                     /locus_tag="PLW07_01935"
     CDS             8586..>8664
                     /locus_tag="PLW07_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MscL family protein"
                     /protein_id="HOL49315.1"
                     /translation="MKMIKEFKEFAMKGNVVDMAIGIIIG"
BASE COUNT         2863 a         1434 c         1995 g         2372 t
ORIGIN      
        1 caaacggata cagaatttta gagaaaaatt acagaacaaa atcaggcgaa atagacatca
       61 tcgcgagaaa aaacagaagt attgtgttca ttgaagtaaa gacacgaagt aatgatagtt
      121 ttggctctcc tgtagaagca gttgatagaa ggaaacttgc gaaacttgaa atcgttgcca
      181 accagtacat tcagaagaaa gaaattgaag acgccgcttt cagatttgaa gttatttcaa
      241 taacaaaaat caataatgcc tggcaatgtg aaataattcc ggtggactga tgcttgcaaa
      301 aattcactca gttgctgtat gggggattga aggatatcct gtgagtgttg aaattgatat
      361 ctcaaaaggt atgccatcag ttgcaatcgt tgggcttcca gaccaggcag tgaaagaaag
      421 cagggatcgt attaaacctg ccatcaaaaa ttcaggattt gaattcccgc aatcaaaaat
      481 cactatcaat cttgctcctg ctgatttgaa aaaagagggt tcctgttttg acctgccagt
      541 tgctcttggt attcttgctg caaggcagat aatatcttct gatgttgctt ccagatttca
      601 ttttattgga gaacttgccc ttgatggttc tgtgaaacct gtaagcggtg ttttgccaat
      661 ggcaatgagc gtgaaacaat caggtggaaa atttgttgtt ccaaaagcca atgccattga
      721 agcgtctgtt gttggaattg aggcatatgg ggtggagagc ttgaaggaat gtgtagattt
      781 tcttaccggg aaaaaagaaa ttttacctta caatgttgat tgggataaca ttcttgcagt
      841 gcagaataag tatgatattg actttaagga agtaaaagga caggcagttg cgagaagggc
      901 aattgaagtg gctgtggcag gtgcgcataa cctgcttgtg attggacctc ctggtgctgg
      961 aaaaagcatg attgcaagaa gggttcccac gatatttcca ccgctgacga ttgaagaagc
     1021 gattgaaatt acaaaaatcc atagtgtcgc tggaatgctt gccggaggta ttgtttccac
     1081 aagacctttt aggcaaccac atcatacaat ttctgatatc gctattattg gtggtggaac
     1141 aattccaaaa ccaggagaaa tatctctagc gcatcatgga gtgctttttc tggatgaact
     1201 tccagaattt cacagggatg ttcttgaatc ccttcgtcag ccacttgaag atggaaaggt
     1261 ttctgtttca cgagcaagag gaagaattga ttttccatca agatttttcc tgattgctgc
     1321 aatgaatcca tgtccctgcg gctggtatgg agacagtcgc aggccatgtc gttgcacagt
     1381 gccacagatt atcaaataca ggaaaaagat atcaggacca ttacttgaca ggatagatat
     1441 tcaaattgaa gtatctggtc ttggctcaaa acaacttgtt tctgatagtg tttcagagga
     1501 ctcagaaagt atcagaaaaa gaatcaccag atgcaggaaa atccagcagg aacggtttgc
     1561 tgggactggt attttttgca atgcccacat gaacacaaag cagattaaac agttttgtat
     1621 tcttgacgat gaatgcagga aatttctgga aaacgcatta gaaaaaatga atctatctgc
     1681 gcgtggttat gacaaagtgg taaaggttgc aagaacaata gcagaccttg aagaatcaga
     1741 aaaaatttca ttaagccata tagcagaagc gcttcaatac aggagttttg acagggaact
     1801 gtggtaggaa gatttatgaa taaaaaagtg aagccaggaa aaattgtaat ggaacttccg
     1861 ccggctgggg aaaatgcaag aaacagcgaa ggaacattca tcacactgaa atcaggaagg
     1921 attgtttttg cctggtcaaa attttttggt tctcattctg attttggcga ggcggttatc
     1981 gcagcaaaat attcagatga tgatggcaaa acatggtcaa aggatgatag ggttttgata
     2041 gaaaaagagg gcacgaccaa tgtaatgagt ccttcctttt tgcggcttca ggatggaaga
     2101 atagccattg tgtatctgcg caaggatggg aaaaaatcct gcatgccata cttccgcaca
     2161 agtgatgatg aactgaagac tttttctgaa cctgtgtcaa ttgcttacga acctggctat
     2221 tatgtcgtaa acaatgaccg tgctattcaa acaaaatgtg gtagaatcgt tgttcctgct
     2281 gcgctgcata gatatcgcgg accatcagtt cttctgccag gtgaagaacc aaaaactttt
     2341 cttgcctcac cggcaattat tctatttttc ttgagtgacg acaacggaaa gcactggatg
     2401 gaatctttga ccaattatta caaatgtttt ccatcaggtc atggacttca ggaaccagga
     2461 gtgattgaac tcaatgacag aaggttgtgg tcatggacaa gacctggaac agaaggaaga
     2521 caatgggaat cattcagtga tgacctgggt caaacatgga cagagcccag accatcacag
     2581 tttgtttcac cgtgcagccc catgcagata aaaagaattc caaaaacagg ccatcttttt
     2641 gctgtgtgga acgaccattc tggaattttc aagacagaaa aaccccaccc tgtctcatgg
     2701 gggagaaccc cgcttgtctg cgcaataagt caggatgaag gaaacacatg gcaacatcac
     2761 aaacttcttg aagattcccc tgaacatggg ttctgttatc ctgccattgg ctttacagaa
     2821 gaagcagtgt taatttccta caacgcaggc ggaataacaa gcaaaattcc tcttgatact
     2881 caaaggataa gaagaatttt gcttgttgat ttgtatgact gaaaaaagat ttatcggaac
     2941 aagcggctgg aattacaaaa tatggcagga tacattcgtt ctgatgaatc ggtataaaca
     3001 aacgcagaca gggtttgttt tttcagggaa agttcacaga agtatcaccc atatcaaaag
     3061 atttattgaa gtggaagaag catggaaaac acaaagatgt ttttgtatat ttcaacaatg
     3121 atttctatgg atatgcggtg aaaaatgcaa aggaacttat tacactgtgc caggagaaaa
     3181 aatgaaaaaa ttgtgtttgt cgttttttat gttgtttgcg gttgcctttt gttatgctgg
     3241 tattaatatc acctacaaag ggcttgataa tatccatatt cagtttgatc accagacaag
     3301 caaacaatgt actgtgaaac ttgtatgcgg tgaaacaata cttttttcac aggttttacc
     3361 acaaggtggt ggttctgtat cagccaatat tcatcacaca ggcctatcaa acggaacata
     3421 cacttataac atttttgaag acgaggatgt tgtatcctca gtatctgaaa caataaatgg
     3481 tcaggtgtgt ggaacattgc tgtttgacga aaccattaat atccctgcgg atgttatatc
     3541 tattaatgtc cccgaaggga aaacgcttga catcaacgct gacatcagtg agattagttt
     3601 ttcttctatt gatgtagttg gcaatatcaa tttacaaaat ggtatcacaa tacaaggtgt
     3661 cacaatttat tttggtggtc agccagtggc tataaggaat atcaagctta cagggtcagg
     3721 cgaattttat ttcaaggaag catcttctgg gtcaagtata gaaaattgtg atgtaagtag
     3781 tgtcagtgtt gacccaaata catcaatcgc ggtgaaaaac tcaaaaatcg ggacaatgct
     3841 tgttgtggac tcggcttcat taaatgcaga agaatcagaa atatctgatc ttttatgcgg
     3901 tggcaacatt gtgttgaata ggtgcagtat taatggagag ttattcctta ttaataatgc
     3961 acaaaatttc actgctacag atacctgttt tttaaacagg gtcaatattg aaggtggaag
     4021 tcctcagttt agcagatgtg aatttttgaa ccagttgcgt ttttatggca gaaaccaggc
     4081 aaattttgac aattgtgtat ttggaaattc tgttagtttt gttgattttg gagtgtttga
     4141 taatccaaag tggtcagata attctggtat gcagccaata ttttctcatt gcagttttgt
     4201 tggacaaact ggtccatatt ttgaatatac tgtgccatct ttgcctgttg accttggtgg
     4261 ctcaaattat tacggagaca aaaaacctga ccttgattgg ttttcaactc ctattgtgca
     4321 taatgagttg ccgaattatc cgcagggtgg aagattctta ctcaggggag tgcctgttaa
     4381 taaacaacat ttcaggattg aacaatggag tacatcagga ccgaatttga cctgcaataa
     4441 aattcttcct gatatatggg caaatgatta tatcgttggt caaaacacga taccatggcc
     4501 aaattatcag aatacatcaa gacagaaagt gttgataaaa ggaagggaaa cactgctttc
     4561 tgtggatgtt gcaaccaact atccactcgt tcagggcgtg aaatttaaag cagtgtttga
     4621 tgggcaggaa atagcacctg ttaatccatc cattgtatta aaaagggata tttcagattc
     4681 ttcaaatgat gttttttatg gaaacaccac cataaatttt attcttcctc caacagagaa
     4741 tgacattgtg aattttacgg ttattatgga tactacaaat atcagtggat ttgacgatgc
     4801 agcaggaaag gccgaaaaag ttctttttgg tggaacactt tcatttgcac cttcttatgc
     4861 aagacaatta aacattcttg ttcaaccggt tcaattgttt atcacaggtt atacaagaaa
     4921 aattcctgat gcttcatcag tcgccagtgc tcttaaaaat ttaataccag caatgctacc
     4981 cattgataga aaagaccttc atatctggac agcaccagtg accactttct atggtggaat
     5041 tttttcaatg ttcagcacaa cagcactgct taatagaata gcaaatgaac ttgcattagg
     5101 acaaggattg gttaatacaa cggcatcagt tggtgactgg ctttcaggaa gagagacagc
     5161 aatgattgat ttcattgtgg cagtgatgcc aaaaggaagt atgggagaag gagtttcagg
     5221 agtcagtctt gcgttaagga gagggatcat atttgttgac gaaagtgcgc cagatgctgc
     5281 attgcacgag atgggtcatg ggattggttt atacacagga acagaacaat atgatcagta
     5341 tccaccagca ggtctttccc ttgaaagaat gacagcattt ctcaatgacg atagggtttc
     5401 aataaacggc ttcaggaaca ggtttttaca ttttccagga acaattcatt cctggtacca
     5461 ggaaaaatac tggtatgaca ttatgggttc ttcaacgaca ttaatctggc caaactggac
     5521 gacttttagc gcatttgcat actatttcaa tgaaaccctt ggaaatgaaa acaaaactgt
     5581 aaaattttta acaaaggcag taccatctgg taataagaga atttttgttt ctgcaacaac
     5641 agagaaaacg aattttaatg gtggatataa aattaaagaa ggcactatca gtgcttttga
     5701 cattactgat ttttctacag gaaaaatcaa tgttcctgtg tactgggatg atgataatat
     5761 tgcagtgaat gattatataa tcaaatgttt tgacagtgca gacaatcaaa tttttaacca
     5821 gccattcaca gtagtttctc cgtatcctgg ttattttggc tgggttaact ggccagaaga
     5881 aagtagttgg tgtggaacat ttgacattcc agaaaacact catttaatag caatctatcc
     5941 aagacctggt tggtggaata acttccagga cacaccaatt ttagaagtga atggagtaga
     6001 ttatgaaccg attaagatta cagggcttaa acccggacag caactcggag agagtgttga
     6061 gatacgatgg acccaggata cgagcaagca atcttcaagc atgaggtatc ttgtgatgta
     6121 cagtgaagat ggagggaaca gctggttacc agcaggaata ccagtagaag gcaataaggt
     6181 ggtcttaccc acagattttc taccagcatc agaggatata attttcaagg tgataggcag
     6241 caatggactt gggacagtag aagatgaggt aggagggttg aagttagaaa acagagcgcc
     6301 aaaggcagtg atagtaacgc cgaaggaagg atgggtggga gaagtaggca caaaatgggt
     6361 attcaaggga tatggagaag atgtggaaga tggggtcata ccacagggac aatggagttc
     6421 ttctatagat ggagagatag atgcatcaaa agaggttgaa ttgagccagg gtattcacac
     6481 gattacattt acagtaaccg atagtggtgg attaacagga agcacaagca ttaccgtaga
     6541 agtgaagcag atgcaggata tagatatcgg gcttaatgaa gacagtttac agttattcat
     6601 tgaagggaaa gacccattga acacagcgcc attggtgtac ttaaaagaaa atggggtcca
     6661 cagggcgata ttaaagatac agaatacagg gacagatacg atattcacag gagccatata
     6721 catcacaaag ccaggagaag cagagcaatt gttggtagaa aagaccttta cagcaggagc
     6781 atttgaagag gtagtaataa gtgagacatt tttagtggta gcgccagggc agtatgagat
     6841 cagagggcag ataaaagaca ttacgccgca ggataccgag gaaggtaata atgaagaagt
     6901 gtggatatac agtacaaagc cagaaagccc tgtgatattg gtagcgccag aggacttaga
     6961 ttttggagat gcaaaggcaa ggaaacaggg acagatactt gtcagcaatt acgggaaatt
     7021 atcgttaaga atcacatcga tgttgatatc aggcgcagat gccaatcaat ttggagtgga
     7081 cgatacgatg ttgcagcagg atatacctga agggaataat gtatttatac ctgtgtattt
     7141 caatccggta tcaacaggga caaagcaggc tctactgaca atcacatcaa atgacattaa
     7201 tcattcgaca atccagatac aattaagagg caattgcatt gggatactgg gagacatttc
     7261 aggagacagt agtgtagata tatcagatgt aattttgtgt ttaaggcagg cgatagggct
     7321 tgatgagcca gagatagatt cagcagatat gaatgaagat gggatgattg atataagtga
     7381 tgtaattctt gtattgagaa aagcaatagg actgtaaaaa catttgacag aacagggata
     7441 gaattgtata acaaaattgt gaacagaaag ttatttagca catcaaaatc tggtcagtcc
     7501 cacaacattt gcctatcagt taatttcatt agaaaagaac agggaggaaa tatgaagaaa
     7561 gtattatgtc tggctgttgc aggattattt ttatgtgttg ttggtgtgtt tgctcaggaa
     7621 ggttatttga gaattacaag caatcctgaa tccgcttctg tggaaattgc tggaaaggtt
     7681 attggcaaga cgcctctcct gacagtattg aaaccaggaa aatatacata caaggtcagt
     7741 ttggctggtt atgaaacagt ttcaggaaca gcagaggttg ttgactatga agtgaccaat
     7801 gtgaatctgg tgcttgtgaa acaggttcag aaagcgcctg ctgcaaaaaa agttgaaccc
     7861 gcaaaaggag gtttgacaat cattactgac tggcaggatg taacggttta tctcaatggt
     7921 cataaaactg ataaggtgcc gcctgttaca atcaaaaatg ttcctgcagg aataaacaaa
     7981 gtaattcttg tcagcggcga ttatgcggat tcgtttcata ttctggtaca gccaggtaag
     8041 acaagtgtat tgaaaaagaa ctttgaagac gataagaaaa aatatgaggc acagatgaca
     8101 actgttgata ccactgttgt tgaaacacct gttgaggtca agaaaagtat gcttccagca
     8161 aaggtgattt tacggctcgg aaccatcgcg agtacagatt caaagaaaca ggatacaaca
     8221 attcttggtg aaagtgatga gatagaagtg agttttcaat acaaaaagac cggtacagat
     8281 gaatggaata caaaaacact tcaatcagga acaaaggtgg aagattcttt tgaaacagaa
     8341 aaaggaacct atgatataca aatcatcgcc actcattaca gggtgccaac aggactgtta
     8401 aatgtcttat tgaccaagaa agaaaaggtc agagaataca aggaaactat taaaaaagag
     8461 attctaccgg atgttcagta cacctatacg attacctatg atggcaagag ttttggctac
     8521 aaacttgaag aggcaaaact caatacacca gttaagtgaa tgttaattta tggatggggg
     8581 ttatgatgaa gatgataaag gaattcaaag aatttgcaat gaagggaaat gttgttgata
     8641 tggcaattgg tattatcata ggtg
//
LOCUS       DAORYU010000033         8658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_5941_length_8658_cov_3.937464, whole genome shotgun sequence.
ACCESSION   DAORYU010000033 DAORYU010000000
VERSION     DAORYU010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8658
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5941_length_8658_cov_3.937464"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PLW07_01940"
     CDS             <1..248
                     /locus_tag="PLW07_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49316.1"
                     /translation="FLRSISAPLSDHSILPYFPSTLNGEQYKISPNPSFIKRGVEGGL
                     IKEGDLTWRGISRNLPQPLFYKEGSSMKWEFLRFDNI"
     gene            complement(262..1392)
                     /gene="ftsW"
                     /locus_tag="PLW07_01945"
     CDS             complement(262..1392)
                     /gene="ftsW"
                     /locus_tag="PLW07_01945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HOL49317.1"
                     /translation="MSSRLRLKIYYRCLIASVILLMLGGAMVLSSSAPYALQTTGDAF
                     FYFKRHCLFFILGLILFFYFRNLHYEKLRKVAKPLIIISLILLVLVLFLGTEVKGAKR
                     AISFGGFRFQPSELAKFALIVYLANFFARYEEQKKNKKLFIIPACVTGLMMALLLKEP
                     DFGSTATIFAYAMAFMIVGGINWMIVVGALLSFVPAIWYLIKSKPYMWKRIVGFLDPD
                     KDPLGIGYHVIQALITIGSGGLWGVGLGRGRQKLEFLPEAHKDYVFAVIGEELGFVGC
                     IAVLALFGTLIFTGFWLSRRIKDTFGKYLAFGISIYFGIQVFLHAGVTMKVLPPKGTT
                     LPFFSYGGSSLVIALSMLGVFFSLIRNAEFESDEPEVISEIL"
     gene            complement(1358..2716)
                     /gene="murD"
                     /locus_tag="PLW07_01950"
     CDS             complement(1358..2716)
                     /gene="murD"
                     /locus_tag="PLW07_01950"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOL49318.1"
                     /translation="MMDIKGKNFLIIGLGETGFDSAVTLARSGANVRVTEIKNNPEIE
                     EKKNELVNLGITVETGAHSIEFVEWADTIIPSPGVGLDAQPIQWAKRINKKILSEIEI
                     AYCLSPSKKIIAITGTNGKTTTVAFTDLLLNKAAMPHVTCGNIGNSFIGEIENIRDDT
                     WIVLEVSSFQLEYIGYFRPYIGVILNIADDHLDYYDNFDQYVRAKSRMFANSQKDDWA
                     ILNYENHWCRKIGEKTNCRTMYFSSKNVIEGVYIENGFVCYDGEKVFSLQQFEQSHLF
                     GIHNLENVMAATGIAEIIGVPPGIIPEVLVEFKPHNHRMETVATINGITFIDDSKATN
                     VDAVCRALESFPGHNNIILILGGKDKHISFEPLKKYLTSRVRMILLIGEAADRIRNEL
                     ESTGIKMEKINNFIEAVDLSMLYGKQGDIVLLSPGCSSFDMFNSYAERGNVFKTAVKN
                     LL"
     gene            complement(2713..3780)
                     /gene="mraY"
                     /locus_tag="PLW07_01955"
     CDS             complement(2713..3780)
                     /gene="mraY"
                     /locus_tag="PLW07_01955"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOL49319.1"
                     /translation="MFHFLVPYITEIFSPYNVFRYITVRTVCASLTSLFICLFLFPSF
                     IRKVKHLSHPKMLEFHQNKEKTPTMGGLLILWSMLISNFLWADLRNIYVLLLILTLCW
                     LGLVGFIDDYLKLTKMNPKGVRPRVKIIWQVSIGFIVGLVLYYHPALNFSSRLYVPFF
                     KNIWFDLGPYCILLITFIIVATSNAVNLTDGLDGLAAGTILMTAVAFGFIAYTVGNAI
                     FAKYLNLPFVQGVGEATVFCGSLVGAILGFLWYNCHPAEIFMGDTGSLPLGGVVGLLA
                     IISKQEVSLIIVGGVFVIEALSVVIQVISFKSRGKRVFLMTPIHHHFELLGWKESQVV
                     VRFWIVAIIFALFTLVTLKIR"
     gene            complement(3782..5134)
                     /gene="murF"
                     /locus_tag="PLW07_01960"
     CDS             complement(3782..5134)
                     /gene="murF"
                     /locus_tag="PLW07_01960"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOL49320.1"
                     /translation="MGPITLDEIVKWTGGSCFSKNDDIVFNSISTDSRTCSCGCLFVA
                     LKGKNFDGHDFIPDAIKAGAKGVIVEKKIPVDIAQILVSDTLKALGDVARNYRRKFLC
                     PVIGITGSDGKTTTKEMCAHLLSSRFNVCKNQGNFNNEIGLPLSIMTMTKNTEAGIFE
                     LGMNGYGQLDYLGRILQPDIVIITSIGYAHLGFFKNRKDLARTKAEILNHIRSDGIAF
                     INGDVNFSDIFNKKLLFRPVYAGLHPANDFQGIITGYQQDQFTLRIKQWEHTDFKIKS
                     WNGAIAYPSLFSILIADYFGIPRDLLPKLVYDFVLIGGRGLVKNAASVRIFDESYNAN
                     PGSMTVALQYFAKQKAKRKLVVVGCMAELGKWSKFYHRRIAELIKKLNFDAVFTIGED
                     ARVISALSNNISKHFESIQDMAEYVCNFVNPGDAIMVKGSRINKLDIIVQHLTGKTGD
                     "
     gene            complement(5141..6619)
                     /locus_tag="PLW07_01965"
     CDS             complement(5141..6619)
                     /locus_tag="PLW07_01965"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOL49321.1"
                     /translation="MKAKELIKNAKLVKPQDILNKEISGIFYDSRKVIPGGIFVAICG
                     STSDGNAFVQDAISRGASIIVTEKKDLLLPSGVGMVVVNNSRRALAEMSAVYYGNPSK
                     KMTIVGITGTKGKTSTSVILKKIFEVASLKTGLIGTIQYEIGQRIVPSTNTTPESIDI
                     QLLLAEMLEHQLTHAVLEVSSHALEQGRVECIDFDAGIFTNISGHEHLDYHKKFSNYL
                     TAKLKFFSHYLPGSQKKNKYAIINIDDAFAKNFISVASENKLNIVTFGLSKKADFYPE
                     QFHFDRSGTSFTVKGKKLFTRLLGISNLYNCLAAIATSSSFGIDLDTISNGLESLHCI
                     PGRMEFIDAGQPFTVVVDYAHTHHALEELLKTIRHLNPRRILLVFGCGGNRDKSKRPL
                     MGKIAAKMADRVYMTSDNPRNESPEAIISDIYRGIPFWRKRKCKLVPDRRAAIETALS
                     EAEENDWVVIAGKGHEQYQIIKSVFHPFDDRKVALEILKRKK"
     gene            complement(6597..8336)
                     /locus_tag="PLW07_01970"
     CDS             complement(6597..8336)
                     /locus_tag="PLW07_01970"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOL49322.1"
                     /translation="MRTQRNINRIDRFIKRQRFVEIFLFLCFCFLAGRLVYLQIFMYE
                     KYSTLADARQYVGKILPAQRGTIYDRNGNILAMDVPVKTLYVDTRHVENKEQTIKVLS
                     TILKMPVRDIEKKFREKYPQIKRKLTVEEYTAIEKERLKGIVFEDDYKRIYPNGVLAC
                     HVLGFVNIDGKGIEGIELQYDDILAGDTGIVTILRDGRGNYLSSLSKTTSRPVNGKDI
                     YLTIDSRIQQIVEEEITNCWETSNPRKISVIVADPHTGEILAMANRPNFDPNTPGKFP
                     AEYRKNYAVTDLFEPGSIFKIVTAAAVLEEKKVNINDVFNCEGGKWFIRGHILHDSHP
                     YGPLDFQHIIIKSSNIGTVKLALKIGEDTLYQYIKKFHFGEPTGIDLPGEIRGILRPV
                     ERWSDYSITAIPIGQEVGVTCLQAIRAISVFANGGYLIQPHILKKIDPPDRLTAQSFE
                     KESVISSTTVSTLNSILARVVSEEGTALKAHIPGYTICGKTGTAQKFENGRYSHSKFV
                     ASFIGFLPLENPKVAILVNVDEPKGAYYGGAVAAPVFKEIAWRVVQYMGIPPQAASIA
                     MRRMDENESKRTD"
     gene            complement(8305..>8658)
                     /locus_tag="PLW07_01975"
     CDS             complement(8305..>8658)
                     /locus_tag="PLW07_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49323.1"
                     /translation="GGVILKKGRMLPHWVSNVALISVFVFCYLIIYCNVIALGYKVSK
                     LEKQYQQLQNWNQYYRSQILKELSLEKVKQRAAKMNLSLEIPAQWRIVDITNIETQLK
                     NGNGNNAYAEEHKQN"
BASE COUNT         2655 a         1907 c         1481 g         2615 t
ORIGIN      
        1 atttccttcg ttccatttct gccccccttt cagaccactc tattctacct tattttccat
       61 ctacccttaa tggggagcag tataaaatct cccccaaccc ctcttttata aagaggggag
      121 tagaaggtgg attaataaag gaaggagacc tgacgtggcg ggggatttca agaaatctcc
      181 cccaacccct cttttataaa gaggggagtt caatgaaatg ggaatttttg cggtttgata
      241 atatttaaaa cgaaatgttc atcacagaat ctcagaaatc acttctggct catcagattc
      301 aaattcagcg ttccggatta aactgaaaaa aacgccgagc attgacaatg caatcacaag
      361 agatgaacct ccgtaactga agaatggaag tgttgtccct ttaggtggga gcaccttcat
      421 ggtcacgcct gcatgaagaa atacctgtat cccgaagtat atgctgattc caaatgcaag
      481 atatttacca aaggtatctt taattcttct gctgagccag aatccggtga aaatcaatgt
      541 tccaaataat gcaaggacag cgatgcaacc tacaaatccc agttcttctc caattactgc
      601 aaacacataa tccttgtgag cctcaggcaa gaattcgagt ttctgccttc ctctgccaag
      661 ccccaccccc cataatcctc ctgaacctat tgtaatcaat gcctggatca catgatatcc
      721 aattccaagt ggatccttgt ccgggtctaa aaatcctact attcttttcc acatgtatgg
      781 cttgctcttg attaaatacc agatagctgg aacaaaagat aatagtgcac caacaacaat
      841 catccagtta atacccccaa ctatcataaa agccatcgca taagcaaaaa tcgtggcagt
      901 gctgccaaaa tctggttcct ttaacaggag tgccatcatc aatccagtta cgcaggcagg
      961 aatgataaat agttttttgt ttttcttctg ctcttcgtat cttgcaaaaa aattggcaag
     1021 atacacgatt aaggcgaatt tggcaagttc ggaaggttga aatctaaaac cgccaaaact
     1081 gattgctctt tttgctccct ttacttctgt cccaagaaaa agcacaagaa caagaagtat
     1141 caaagaaata ataatcagtg gctttgccac tttcctcaat ttctcataat gtaaatttct
     1201 gaaataaaaa aataaaatca acccaagaat aaaaaacaga cagtgtcttt taaaataaaa
     1261 aaaggcatcc cctgttgtct gcaaagcata gggagcgctt gaactcagaa ccattgcgcc
     1321 acccagcatc aaaaggatca cagatgcaat aaggcaccta tagtaaattt ttaaccgcag
     1381 tcttgaagac atttccgcgc tccgcgtaac tgttaaacat gtcaaatgaa gaacatcctg
     1441 gtgaaagcaa aacaatgtca ccttgttttc cgtacaacat tgacaggtcc actgcttcaa
     1501 taaagttgtt aattttctcc atcttaatcc ctgtggactc gagttcattt cttatacggt
     1561 ctgctgcttc tcctatcaga agtatcattc ttacccttga cgtcagatat ttctttagtg
     1621 gctcaaaaga aatatgttta tcctttccac cgagaatcaa aatgatattg ttgtgtcccg
     1681 gaaaactctc aagcgccctg caaacagcat caacattcgt cgccttggaa tcatcaatga
     1741 atgttatgcc attaattgtt gcaactgttt ccattctatg gttgtggggt ttgaattcaa
     1801 caagaacctc gggaattatt ccaggaggaa cgccaattat ttcagcaatt ccagtcgcag
     1861 ccataacatt ttccagattg tgaataccga acaaatgact ttgttcaaac tgttgtagcg
     1921 aaaaaacttt ttcgccatca taacaaacaa aaccattttc aatatatacg ccttcaataa
     1981 cattcttgct tgaaaaatac attgttctgc agtttgtttt ttctccaatt ttccggcacc
     2041 agtgattttc atagttcaaa atagcccaat cgtccttttg tgaattagca aacattctac
     2101 tctttgctct aacgtattgg tcaaagttat cataataatc aagatggtca tcagcaatat
     2161 ttaagattac tccaatataa ggcctgaagt atccaatgta ttctaattga aaactgctga
     2221 cttcaagaac aatccaggtg tcgtcacgta tattttcaat ttcaccaatg aaactattac
     2281 ctatattgcc acaggtaaca tggggcatag ctgccttgtt caataacagg tccgtaaaag
     2341 caacagttgt ggtttttcca ttggtcccag taatagcaat gatttttttt gaaggagaaa
     2401 ggcaataagc aatttctatt tcgctcaaaa tttttttgtt gattctttta gcccattgta
     2461 tgggttgcgc atcaagacca acgcctggac tcgggataat agtatctgcc cattcaacaa
     2521 attcaatgga atgtgcacct gtttcaacag taataccgag atttacaagt tcatttttct
     2581 tttcctcaat ttcagggttg tttttaatct cagtcacacg aacatttgcg cctgatctgg
     2641 caagagtcac ggctgaatca aaaccggttt caccaaggcc gatgataaga aaattctttc
     2701 ctttaatgtc catcatctaa ttttcagtgt cacaagggta aataaagcaa aaataattgc
     2761 cacaatccag aaccgcacca caacctgaga ttccttccat ccaagcaatt caaagtgatg
     2821 gtggattggt gtcatcaaaa aaactctttt acctcttgat ttaaaactta tcacctgaat
     2881 cacaactgaa agtgcttcaa taacaaatac acccccaaca ataataagag aaacttcctg
     2941 tttactgatg attgcaagta atccaacaac tccgcctaaa ggcaaggaac ctgtatcccc
     3001 cataaaaatt tcagcaggat gacagttata ccacaaaaac cccagtatcg ccccaaccag
     3061 agatccgcaa aaaacagttg cttcacccac tccctgaaca aaaggaaggt ttaagtattt
     3121 cgcaaaaatt gcgtttccca ctgtataagc aataaaacca aaagcaaccg ccgtcataag
     3181 aatcgttcca gcagcgagcc catcgagacc gtcagtaaga ttcacagcat tagaagttgc
     3241 cacaataatg aatgttatga gcaagataca gtaaggaccc aggtcaaacc agatgttttt
     3301 gaaaaatggc acatacaaac gacttgaaaa attcaacgca gggtgataat aaagaacaag
     3361 cccgacgata aatccgattg aaacctgcca tataattttt actcgcggcc ttactccttt
     3421 tggattcatc tttgtcagtt tcagataatc gtcaatgaaa ccaacaagtc ccagccagca
     3481 aagagttaga atcaacagta gcacataaat atttcttaaa tccgcccata gaaaatttga
     3541 aataagcatg ctccacagaa tcagcaaacc acccatggtt ggcgtttttt ccttattctg
     3601 gtgaaattcc agcatttttg gatgagatag atgcttcacc ttgcgaatga atgaaggaaa
     3661 caaaaaaagg catataaata gagatgtcag tgaagcgcaa actgtcctta cagtgatata
     3721 acgaaagaca ttgtaaggag aaaaaatttc agttatgtat ggaaccaaaa aatggaacat
     3781 atcagtcccc cgtttttcca gtaagatgtt gaactataat gtccagttta tttatccttg
     3841 aaccttttac cattattgca tcgcccggat taacaaaatt gcacacatat tctgccatat
     3901 cctgaatgct ttcaaaatgc ttgctgatat tgtttgataa agcagagata actcttgcat
     3961 cttcgccaat tgtgaacacg gcatcaaaat tcaatttctt tataagttca gcgattcttc
     4021 tgtgataaaa tttgctccat ttaccaagtt ctgccataca accaaccaca acaagttttc
     4081 ttttcgcttt ttgttttgcg aagtattgaa gggcaactgt catcgaacca gggtttgcgt
     4141 tgtaactttc gtcaaatatt ctcacactgg ctgcattttt caccagtccc cttccaccaa
     4201 tgagaacaaa atcatagacc agttttggca ataagtcacg aggaatacca aaataatcag
     4261 caataagaat tgaaaagagt gatggatatg caattgcgcc attccacgat tttattttga
     4321 aatcagtatg ttcccattgt ttaattctga gtgtaaactg gtcctgttga tacccggtaa
     4381 taattccctg gaaatcattt gcaggatgca aaccagcgta cacaggacga aacaaaagtt
     4441 ttttattgaa aatatcagaa aagtttacgt ccccattaat gaaggcaata ccatcggaac
     4501 gaatatgatt gagtatttct gcttttgttc tggcaagatc ttttctgttt ttgaaaaatc
     4561 ccagatgggc gtaacctatg gatgtaataa taacaatgtc tggctgaaga atcctgccca
     4621 gataatccag ttgaccgtat ccgttcatcc cgagttcaaa aatgcctgct tctgtgtttt
     4681 ttgtcatcgt catgatactc aaaggaagtc ctatttcatt attaaaattc ccctggtttt
     4741 tgcatacatt gaaccgactc gaaagaagat gcgcacacat ttccttggtt gttgtttttc
     4801 catcgctacc tgtaattccg atgacaggac acaagaattt tcttctataa tttctggcaa
     4861 catcacccag ggctttcagg gtatctgaaa ccagaatctg ggcaatatca acaggaattt
     4921 ttttctcaac aatgacacct tttgcgcctg ccttaatagc atcaggaata aaatcatgtc
     4981 cgtcaaaatt ttttcccttc aaagcaacaa aaagacaacc gcaggaacac gttcttgaat
     5041 cggtgctgat gctgttaaaa acaatgtcat catttttaga aaaacaagat ccgcccgtcc
     5101 acttcacaat ctcatcaagt gtaataggtc ccatgtaata ttattttttt ctcttcagta
     5161 tttccagcgc gacctttctg tcgtcaaatg gatgaaaaac acttttgata atctggtatt
     5221 gctcgtgtcc tttaccagca ataacaaccc agtcattttc ctctgcttcg gagagcgctg
     5281 tctctatggc agcccgcctg tccggaacaa gtttgcattt tctttttctc cagaatggaa
     5341 ttccgcgata aatgtctgat attattgctt caggggattc gttgcgcgga ttgtcgctgg
     5401 tcatgtaaac cctgtcagcc attttagcgg caatctttcc catcaatggc cgtttgcttt
     5461 tatctctatt tccaccgcat ccaaaaacaa gaagaatcct ccgtggattc aaatgtcgta
     5521 ttgtcttcaa aagttcttca agagcatgat gagtgtgagc ataatcaacg acaactgtaa
     5581 atggctgtcc tgcatcaata aattccattc ttccaggtat gcaatgcaaa ctctctaacc
     5641 cattcgaaat agtatcaaga tcaatgccaa atgaactgct ggtggcaatc gcggcaagac
     5701 agttgtaaag attactgatg ccaaggagtc gtgtaaacag ttttttccct ttcacagtaa
     5761 aactggtacc agacctgtca aaatgaaatt gttcaggata aaaatcggct ttttttgaca
     5821 aaccaaaggt aacaatattc agtttgtttt ccgacgccac actgataaag tttttcgcaa
     5881 acgcgtcgtc aatattaata attgcatatt tattcttttt ctgactgcct ggcaggtaat
     5941 gcgagaaaaa cttcaatttt gcagtaagat aattggaaaa ttttttgtga taatcaagat
     6001 gttcgtgccc tgaaatattt gtaaaaattc cagcgtcaaa atcaatgcat tcgaccctgc
     6061 cctgttcaag agcatgagat gacacttcca ataccgcgtg agttaattga tgctcaagca
     6121 tctctgcaag caatagttga atgtctattg attcaggcgt agtgtttgtt gaaggaacaa
     6181 ttctctgccc tatctcatac tgaatcgttc caatgagacc agtttttaat gaagccacct
     6241 caaaaatttt cttgagaatc acagaagtac ttgtttttcc tttggtacca gtaattccaa
     6301 caattgtcat tttttttgaa ggattgccgt aataaactgc agacatctca gcaagcgccc
     6361 tgcggctgtt gttaacaact accataccaa caccagaagg aaggagtaag tccttttttt
     6421 ctgtgactat tatgctggct cctcgtgata ttgcatcctg gacaaacgca ttcccatcag
     6481 aagtgcttcc gcaaattgca acaaaaatcc caccaggaat tacttttcgc gagtcataaa
     6541 atatcccgga tatttctttg ttcaaaatat cctggggttt gacaagtttt gcgtttttaa
     6601 tcagttcttt tgctttcatt ttcatccatc cttctcattg caatacttgc tgcctgaggt
     6661 ggaattccca tatactgcac aacacgccat gctatctcct taaacacagg ggctgcaaca
     6721 gcacctccgt aataggcacc tttcggttca tcaacattta caagaatagc aactttcgga
     6781 ttttccagtg gcaagaatcc aataaacgaa gcgacaaatt ttgagtgaga atatcttcca
     6841 ttttcaaatt tctgtgctgt accggttttc ccacaaatgg tataaccagg tatgtgcgcc
     6901 ttcagcgcag taccttcctc gctcacaact cttgcaagaa ttgaattaag cgtggagaca
     6961 gtggtgctgg aaattaccga ttccttttca aaagattgcg cggtcaagcg atcaggaggg
     7021 tcaatttttt tcaggatatg aggttgaata agatatcctc cattggcaaa aacagatatc
     7081 gccctgattg cctgcaaaca ggtgactcca acttcctgac ctattggtat cgctgtaatt
     7141 gaataatcag accatctttc tacaggtcta agaatgccgc gtatttcacc cggcaaatct
     7201 atgccagtcg gttctccgaa atgaaatttc ttaatatact gataaagcgt atcttcacca
     7261 atttttaaag ccagttttac agttccaata ttgcttgact taatgataat gtgctgaaaa
     7321 tcaagtggcc cataaggatg cgagtcgtga agtatatgac cacgtatgaa ccatttgcca
     7381 ccctcgcaat tgaaaacatc gttgatatta accttttttt cttcaagaac agcagcagcc
     7441 gtaacaattt tgaagataga ccctggttca aacagatcag tcactgcata attttttctg
     7501 tattcagcag gaaattttcc tggtgtgttt gggtcgaaat tcggtctgtt tgccatcgcg
     7561 agaatttccc ctgtatgggg atctgcaaca ataacactga tttttctagg attacttgtt
     7621 tcccagcaat ttgtgatttc ttcttcaaca atttgctgaa tacgagaatc tatcgtcaaa
     7681 tagatatcct ttccgtttac tggacggctg gttgttttgc ttaatgaact gagataattg
     7741 cctctaccat cacgcaaaat tgtgacgatt ccggtatctc ctgcaagaat gtcatcgtac
     7801 tgaagttcaa ttccttcaat ccccttccca tcgatgttca caaaaccaag aacatgacat
     7861 gcaagcactc cattagggta aatccgcttg taatcatctt caaaaacaat acccttcaac
     7921 ctttcttttt caatcgcagt gtattcttca actgtaagtt ttcttttaat ttgcgggtat
     7981 ttctctctaa atttcttttc aatgtccctg acaggcattt tcaaaatcgt ggaaagaacc
     8041 tttatcgttt gttccttatt ttctacatgt cgcgtatcaa cgtacagtgt cttcacaggt
     8101 acatccattg caagaatatt accatttctg tcatatatcg tgcccctctg ggcaggtaat
     8161 atcttgccta catactgtct tgcatctgcc agcgtggaat atttttcgta catgaaaatt
     8221 tgaagataca ccagccgtcc cgcaagaaaa caaaaacaaa gaaacagaaa tatttcaaca
     8281 aacctctgtc tttttataaa tctatcaatt ctgtttatgt tcctctgcgt acgcattgtt
     8341 tccgtttcca ttttttaact gcgtttcaat gtttgtaata tccacaattc tccactgggc
     8401 aggaatttca agagaaagat tcattttcgc tgcacgttgc ttcacttttt caagggacag
     8461 ttctttaaga atctgactgc gataatactg attccagttt tgcaactgct gatattgttt
     8521 ttcaagttta gaaactttgt atccaagagc aattacatta cagtaaataa tcagataaca
     8581 aaaaacaaaa acagatataa gggcaacatt tgaaacccag tgaggcaaca ttcttccttt
     8641 tttcaaaata acacctcc
//
LOCUS       DAORYU010000034         8651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_5943_length_8651_cov_6.131340, whole genome shotgun sequence.
ACCESSION   DAORYU010000034 DAORYU010000000
VERSION     DAORYU010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8651
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5943_length_8651_cov_6.131340"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1864
                     /locus_tag="PLW07_01980"
     CDS             <1..1864
                     /locus_tag="PLW07_01980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOL49324.1"
                     /translation="TTIFCRFGSMSSIIMCSIIAYAIARHKILNISVFAEKTFVYGCL
                     IAGLMLIYAFFVWSLSHFVRIFTTETSLVPVIASSFIIAIAFSPLKEIIQRWARKKIF
                     KQQYDIEQILTQMRWIIGSSLNLEEGIAAMMEIIDNEIHIKNMPVFLIKPDQAAKKIF
                     MELKRNSVLRFEFDDTAPILRLLETEPFTHFKDELIRFSSRDIINSVLRQMDFYGAEV
                     IIPIHFQNRIIGAILLGGKDDETSFSNTERRILNALSLYMGMFVETVALATTLRENRS
                     YQQSLLEKLPSGVIAVDDEHDVIVFNQEAERITGMKKENVLRKKYDDVIPYDLQKLLS
                     SLLQNKEEIRHIETEFSKNGCVIPLMASGACFYSSRGSLLGAQLIFSDISQMKTLQNQ
                     VKRNERLASLGILAAGIAHEIRNPLVALKTFSQLLPEKFSDEEFRVNYTKVVIPEIER
                     IDGLVEQLLVFARPRPVRKEKTDLVSIIESTIMLIKAQARFASISFVGQYEKKEVEIV
                     ADSEKLKQALWNILLNSAEAMEENKGTIKIQVMEGSDTVVVTIEDDGCGIPEENANKI
                     FEPLFTTKPNGTGLGLPIVNEIIAQHNGRIFVESKKGVGTKVTIELPLNERVEK"
     gene            1861..3753
                     /locus_tag="PLW07_01985"
     CDS             1861..3753
                     /locus_tag="PLW07_01985"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOL49325.1"
                     /translation="MKYVMIVVEETGIANALNMLLKDKFFPVVIMPDNVRQSIVQRKP
                     DIVIIDSLYSSISGYELAEEIIKMIPDVPVIALVDSYGPVARRLMNLGVYEVIEKPFD
                     PERFMYALNRAAAHVEIRKPETLETVQNDRDRVSLITENTFFQKLSELIAENFSVPER
                     LIYAMINLLRTQFSLSGISLFICKSDEFVFYDGIGVEKSFLKNVRFNPGSAIYRWLMS
                     ERKIIQKNHSYSSDLISEMNLLQADLILPLLNRDGLALGFFAFGTRLTGELFTAETIR
                     FLTVTTTYLSVLIEDAFLFQDIVIQKESQKIILENVPTGIIVLDNACNVKIFNKQAES
                     ILGKRIRDVINLSVEHCGAEFASKVKDVIVNHQPISREELFISTLRKWLGMSCDFIKQ
                     NNEIIWTIIIFQDITSARNLEKERRRVEQNQYWQQIAQQLSHEIKNPLVAIKTFACLL
                     PEKFSDETFRTEFYKIVNGEIQRLTFLVEKIARLADTEQLILNKTNCLEILQKVKQKF
                     PSVEITSRDGVGIVTKADATRLQEALELLLDFCVNDAGEQENITISLTVQDDCIEISI
                     LETGNNIRCQTGQDLFTPFSNNLSALHSLNLAICRKIIEEHSGRIFSEILPESKKFII
                     KLPVPK"
     gene            3750..5117
                     /locus_tag="PLW07_01990"
     CDS             3750..5117
                     /locus_tag="PLW07_01990"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOL49326.1"
                     /translation="MTKKILVIDDEKSVQESLKLVLSDNWRVLVASSGREGLEILSNN
                     EIDIIFLDILMPDRDGLEVLQEIKKIDETIPVIMLTAVSKLSTAIEAMKIGAYDYLTK
                     PFDVEEIRLIVQKIFNEFKMRQRVQFLTDEMEKSTETIIYKSDPMKEVLSLALRAAPH
                     DSAVLITGPTGSGKELIARFIHRESPRKNEPFIAVHCAAIPETLFESEIFGYEKGAFT
                     GAFKSKPGKIEVAGSGTIFFDEIGEMPLSLQIKLLRVLQEHEFSRVGSNEPIKMEARV
                     LSATSRDLKKEVESGRFREDLFYRLSVIPIAIPPLKEREQDILPIAYHFLSFFGKQTG
                     ARAKMFSSEAEQCLLNYDWPGNVREVKNVIERILVLRGNKDIINIEDLPEELRVVRSG
                     NSEDASFEERVIQFEKRLIFEALKKFSWNKSKAAEYLRMSRRVLNYKVEKYGIRERQE
                     KNSGY"
     gene            5077..6480
                     /locus_tag="PLW07_01995"
     CDS             5077..6480
                     /locus_tag="PLW07_01995"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOL49327.1"
                     /translation="MESEKGKKKILVIEDNIGVQNLYRDILKKHSYETEILDSVSGAI
                     DALKKTNFDLVILDLQLSEENGLMFLDNLKSLETSVPPVIVCTGMASIENAVNAMKKG
                     ASDFITKPFTVDVLISTIERNISISELSKKISEIEMIRSILELNRIIISITDIEDLCT
                     GVIDLVYNLVKPDVALLYFSDDDTGRFFLRRTKGNTADFNPPVVLSLSETGDFHRKNN
                     DIVHRIDEKSFEVKLFGRKNIIGILRFYSTERFDDRELNFLSVFGTQMGIALENANLL
                     QQLQESYISAIRSLVNSLEAKDAYTKNHSEQVAYYAVAIGKVLGLDQKSLESLRNAAY
                     LHDIGKLGIKDEIILKKSSLSEKELEIIKKHPLITKEILQPLKMRKEEIEACLYHHER
                     VNGKGYPEGLSGEKIPLFARILAVADSLSAMISERPYRKKMEIEDAIEELKRNAGQQF
                     DENIVKVLIHVVENSKK"
     gene            6502..7527
                     /locus_tag="PLW07_02000"
     CDS             6502..7527
                     /locus_tag="PLW07_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOL49328.1"
                     /translation="MISISRLLSNDLGIDLGTANTAVYVRGKGVVLMEPSIVAINRDT
                     KEVLAVGIEAKKMLGKTPANIIAMKPMKDGVIADFDACESMLRHFINKVKSPRHKFVM
                     PPRMVIAVPSGITSVERRAVRETALKAGARMVKLVEEPMASAIGVGLPVEEPCGSFIL
                     DIGGGTTEMAVISLGGLVLTKSLRVAGNEMDEAISNYLKKKYNLLIGEKTAEDIKINI
                     GSAFSLEEELTMEVHGRDLVEGLPKMIRIRAEEIREALQETLMAIVGAVRDILEETPP
                     ELSSDLVERGLVIAGGGALLRNIDKLISQETKLPVVIAENPLLAVVTGAGKMLEEDRY
                     LKMFPSE"
     gene            7536..8219
                     /gene="mreC"
                     /locus_tag="PLW07_02005"
     CDS             7536..8219
                     /gene="mreC"
                     /locus_tag="PLW07_02005"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOL49329.1"
                     /translation="MLWRFRREIIFIILIMLGAWLIRHSVSFRMFFPKHTVSSGNFQQ
                     DVESIINENEQLKKLLGLKQSGSFTKIIYASVKTISPWVFPSSIVFDKGTEDGVKEGM
                     SVISSSGSLIGRVISVNKNNSTGITLYHPDSKVSVMVLSTGELAVIEGVSVASLYPYL
                     RIKFLPPECKASAGDIVETSGLTRLFPHHIRVGRIVKINNSSSEPITHGVVVPFFINE
                     NIKRVALVE"
     gene            complement(8310..>8651)
                     /locus_tag="PLW07_02010"
     CDS             complement(8310..>8651)
                     /locus_tag="PLW07_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49330.1"
                     /translation="YPLRRLYLIIIKMKHIAICSIINQNEKRIIIEIFHIQPPETRQN
                     FTIRYNKQLIAAETILVISVSILKEENKYQIVPSASISLPGLSPVMIENIMLHKKTIK
                     KNGNIFLVKAT"
BASE COUNT         2819 a         1309 c         1890 g         2633 t
ORIGIN      
        1 aacaaccatt ttctgtcgat ttggatcaat gtcttccata attatgtgct ctattattgc
       61 ttatgcaatc gcaaggcaca aaattttgaa catttctgtt ttcgctgaaa aaacatttgt
      121 ttatggttgt ctgattgcag gattgatgtt gatatacgcg ttttttgtct ggtctttatc
      181 gcattttgtc agaatattta ccactgaaac atcacttgtc cctgtgattg cgagttcttt
      241 tatcatagca attgcttttt cgccattgaa ggaaattatt cagagatggg caagaaaaaa
      301 gatcttcaaa caacaatatg atatagaaca aattttgact caaatgagat ggattatagg
      361 aagttcttta aatcttgaag aaggtattgc tgcaatgatg gaaataattg acaatgaaat
      421 ccatattaaa aatatgcctg tttttcttat aaaacccgat caggcagcaa agaaaatttt
      481 tatggaatta aagagaaatt ctgtgctcag gtttgaattt gatgacactg ccccgatttt
      541 aagattgctg gaaacagaac cttttacgca ttttaaggac gaacttatta gattttcgag
      601 tagagacatt atcaattctg tattgaggca gatggatttc tatggagcag aagttatcat
      661 accgattcac ttccagaaca gaataattgg agctatttta cttggcggca aggatgatga
      721 aacaagtttt tcaaatactg aaagaaggat attgaatgcg ttgtcattgt atatgggaat
      781 gtttgttgaa acagttgctc ttgccaccac ccttagagaa aatagaagtt atcagcagtc
      841 gttacttgaa aaactgccga gtggcgttat tgctgtggat gatgaacacg atgttattgt
      901 ttttaatcaa gaagccgaaa gaattacagg aatgaagaag gaaaatgtgt taaggaaaaa
      961 atatgatgat gtaataccat atgatttaca aaaattgttg tcatctcttt tgcaaaataa
     1021 agaggaaatt agacatattg aaacagaatt ttcaaaaaat ggttgtgtga ttcctttaat
     1081 ggcaagtgga gcatgttttt attcatcaag aggttctctc cttggggccc aattaatttt
     1141 ttctgatatt tctcaaatga aaacgcttca gaaccaggtg aaaagaaatg aaaggctggc
     1201 ttctcttggt attcttgctg caggtattgc acatgaaatt agaaacccgc ttgtggctct
     1261 aaaaactttt tcgcaactat tacccgagaa attttcagat gaggaattta gagtaaatta
     1321 tacaaaagtt gtgattcctg aaattgaaag aattgatgga cttgtggagc aactgcttgt
     1381 ttttgcaaga ccaaggccag taagaaagga gaaaacagac cttgtttcaa taatagaatc
     1441 aacgattatg ctgataaaag cgcaggcgag atttgccagc atttcttttg ttggacaata
     1501 cgagaaaaaa gaagttgaaa tcgttgcaga ttctgaaaaa ttaaagcagg ccttatggaa
     1561 tattttgtta aatagtgcag aggcgatgga agaaaacaaa ggaaccatca aaattcaggt
     1621 gatggagggt agtgacacgg ttgttgtgac tattgaagac gatgggtgcg gaattccaga
     1681 agaaaacgca aataaaattt ttgaaccgct gttcacgact aaacccaatg gaacagggct
     1741 tggtttgccg atagtaaacg aaattattgc tcaacacaat ggtagaatct ttgttgaaag
     1801 taaaaaggga gtcggaacaa aggtaacgat agaactgcct ttgaatgaga gggttgaaaa
     1861 atgaagtatg tgatgattgt tgtggaagag acaggcatag caaatgcttt aaatatgttg
     1921 ttgaaagata agttttttcc tgtggttata atgccagata atgtccgaca atcaatcgtg
     1981 caaagaaaac cagatatagt gataatagat agtttatatt ccagcatatc agggtatgaa
     2041 ctcgctgagg aaattataaa aatgattcct gatgttcctg tgattgccct tgttgattcc
     2101 tatggacctg tggcacggcg tttgatgaac cttggtgttt atgaggtgat tgagaaaccg
     2161 tttgatcctg aaaggtttat gtatgcactg aatcgagccg ctgcccatgt cgaaattaga
     2221 aagccggaaa cactggaaac agttcagaat gatcgcgaca gggtatccct gataactgag
     2281 aacacatttt ttcaaaaact gagtgaactg attgctgaaa atttttcagt ccctgaaaga
     2341 ttgatttatg caatgattaa cctgttacgg actcaatttt cattatccgg aatatctctg
     2401 ttcatttgca aatctgatga gtttgtgttt tatgatggga taggagttga gaaaagtttt
     2461 ttgaaaaatg tcagatttaa ccccggtagt gccatttaca gatggctcat gtcagagaga
     2521 aaaattattc aaaaaaacca ttcttattca tctgatctga tttcagaaat gaatctgtta
     2581 caggcagatc tgatacttcc tcttttgaac agagacgggc ttgctctggg attttttgca
     2641 tttggaacca ggttaaccgg agaacttttt accgctgaaa caatcagatt tttgacagta
     2701 acgacaactt atttgagtgt attgattgaa gatgctttcc ttttccagga tatcgtgatt
     2761 cagaaagaat cacagaagat catacttgaa aatgtcccta ccgggattat tgttcttgat
     2821 aacgcctgta acgtaaaaat tttcaacaaa caggcggaat caatactcgg gaaaagaatc
     2881 agagatgtga tcaatttatc tgtggaacat tgcggggctg aatttgcgtc aaaagtgaaa
     2941 gacgtgattg tcaatcatca gccaataagt agagaagagt tatttatttc aacattgaga
     3001 aagtggcttg gtatgagttg tgattttatt aaacagaaca atgaaattat atggacgata
     3061 ataatttttc aggatattac ctctgcaagg aatctggaaa aagaaagaag aagggttgaa
     3121 cagaatcaat actggcagca aattgcacaa cagctttctc acgaaataaa aaatcctctg
     3181 gtggcaataa aaacctttgc ctgtcttctt cctgaaaaat tttcagatga aacttttcga
     3241 acagaattct ataaaatcgt gaacggagaa attcagagat taacatttct tgtagaaaag
     3301 attgctcggc tggctgacac agaacagttg attttgaata aaacaaattg tctggaaatt
     3361 cttcaaaagg tgaaacagaa atttccatca gtagaaatta cttctcgtga tggagtaggt
     3421 attgtgacaa aagcagatgc aacaagactt caggaagcac ttgaattact gcttgatttt
     3481 tgcgttaatg atgctggaga acaagaaaat atcacgattt ctttaactgt ccaggatgat
     3541 tgtattgaaa tttcaatatt ggaaacaggt aataatatta gatgtcagac cggacaggat
     3601 ttatttacac ctttttcaaa taatctttcg gcattgcatt ctcttaatct ggcaatatgt
     3661 cgaaaaatta tagaggagca ttcaggcaga atattttcag aaattttacc agagagtaaa
     3721 aagttcatta tcaaacttcc agttccaaaa tgacaaagaa aattcttgtt attgatgacg
     3781 aaaaaagcgt gcaggaatct ttgaaacttg tcctttcaga taactggcga gtcctggttg
     3841 cttcttcagg cagggaagga cttgagatat tatcaaataa tgaaattgat attatttttc
     3901 tggatatatt gatgcctgat agagatggtc tggaggtttt acaggagatt aaaaaaattg
     3961 atgaaacaat tcctgtgatt atgcttacgg ctgtttcaaa actgtccaca gccattgaag
     4021 caatgaagat tggcgcatat gattatctga caaaaccgtt tgatgtggag gaaattcgtc
     4081 ttattgtgca gaaaatcttc aacgaattta agatgagaca gagggtacaa tttctgactg
     4141 atgagatgga gaaatcaact gaaaccatta tatataagag tgacccgatg aaggaggttt
     4201 tgtcgcttgc tctgcgggcg gcgccccatg attctgcggt tttaattaca ggaccgacag
     4261 gcagtggaaa agaattaata gcaagattta ttcataggga aagtccaagg aaaaatgaac
     4321 catttattgc tgttcattgt gctgctatac ctgaaactct ttttgaaagt gaaatttttg
     4381 gatatgaaaa aggagcattt actggagcat tcaagagcaa accaggaaaa attgaagtgg
     4441 cgggttctgg aaccattttt tttgatgaaa ttggggaaat gccactttct cttcaaatta
     4501 aactactacg cgtattacag gaacacgaat tttcaagggt aggatcaaac gaacccatca
     4561 aaatggaggc aagggttctt tccgcgacct caagagattt gaaaaaggaa gtggaatccg
     4621 gaagattcag agaagatttg ttttacaggt tgagtgtgat tcccattgcc ataccgcctc
     4681 ttaaagaaag ggagcaggat attcttccga ttgcatacca ttttctttct tttttcggaa
     4741 aacagactgg agcaagggca aagatgtttt catccgaagc ggaacaatgt ttgttgaatt
     4801 atgattggcc cggcaatgtg agagaagtaa aaaatgtgat agaacgtatt ttggttttaa
     4861 gaggaaacaa ggatataata aatatagaag atttaccgga agaattaaga gtagtacgat
     4921 ccggaaattc agaagatgct tcctttgagg aaagagtgat acagtttgaa aaaagattga
     4981 tttttgaggc gcttaaaaaa ttctcatgga ataagtcaaa ggcagcagaa tatttaagaa
     5041 tgagtcgtag agttctgaac tataaggttg aaaaatatgg aatcagagaa aggcaagaaa
     5101 aaaattctgg ttattgaaga caatatcggg gttcaaaacc tgtacaggga tattttaaaa
     5161 aagcattcgt atgaaacaga aattctggat agtgtttctg gagcaatcga cgcattgaag
     5221 aaaacgaatt ttgaccttgt gattcttgat ttacaactgt ctgaagaaaa cgggctgatg
     5281 tttcttgaca atttgaagag tcttgaaacc agtgttcctc cagttattgt gtgcacgggc
     5341 atggcttcaa tagaaaatgc agtgaatgca atgaaaaaag gtgcaagtga cttcattacc
     5401 aaaccattca ctgttgatgt tttaatatct accattgaac gaaatatcag tattagtgaa
     5461 ctgtcaaaga aaatttcaga aatagaaatg attcgttcaa ttcttgaatt gaacaggatt
     5521 atcatttcca ttacagatat tgaagatctc tgtacaggtg tcattgacct tgtttataat
     5581 cttgtgaaac cagatgttgc actattatat ttcagtgatg acgataccgg aagatttttt
     5641 ttaagacgga cgaaaggaaa tacagcagat tttaatcctc cagtagttct ttctctatct
     5701 gaaacaggcg atttccacag aaaaaataat gatattgttc atagaataga tgaaaaatct
     5761 tttgaagtaa aactttttgg gaggaaaaac atcattggta tactgagatt ttattccacc
     5821 gagagatttg acgacaggga actgaatttt ctttcagtgt ttgggaccca gatgggtatt
     5881 gctcttgaaa acgcaaattt gcttcagcaa cttcaggaat cctacataag cgcaataagg
     5941 tctctggtaa attcccttga ggcgaaggat gcatatacaa agaaccattc tgaacaggtt
     6001 gcatattacg ctgttgcaat tggtaaggtt ctgggattgg accagaaatc tcttgaaagt
     6061 ttgcgcaacg ctgcatacct ccatgatatc ggaaaattgg gtataaagga tgaaattatt
     6121 ttgaagaaat cttcactttc agaaaaagaa ttggaaatta taaaaaagca tccgcttatt
     6181 acaaaagaaa ttcttcagcc gctaaaaatg aggaaagaag aaatagaggc atgtctttat
     6241 catcatgaaa gggttaatgg taagggttat cctgaaggac tttcaggtga aaagattcct
     6301 ctatttgcaa gaattcttgc agtggctgac tctctgagcg caatgatttc tgaaagacct
     6361 tatagaaaga agatggaaat agaggatgcc atagaggaat tgaaaagaaa cgctgggcag
     6421 cagtttgatg agaacattgt aaaagttttg attcatgttg ttgagaattc aaaaaaataa
     6481 tttcactgaa aggagtattt catgataagc atttcacgac tactgtcaaa tgatctcggc
     6541 attgacctgg gaactgccaa tactgcagtt tatgtaagag gaaaaggtgt tgtattgatg
     6601 gaaccatcta ttgtggcaat caatcgggat accaaagaag tacttgcggt tggaatagaa
     6661 gcaaagaaaa tgcttggtaa aactccggca aacatcattg cgatgaagcc aatgaaggat
     6721 ggtgttattg ctgattttga cgcttgtgag tcaatgctga gacattttat taataaggta
     6781 aaatcaccaa gacataagtt tgttatgccg ccgagaatgg ttatcgcggt accctctgga
     6841 attacaagcg ttgaaaggag agcagttcgc gaaaccgctc tgaaagcagg cgcgagaatg
     6901 gtaaaacttg tggaagaacc gatggcttct gcgattggag ttggattacc tgtcgaggag
     6961 ccgtgtggca gttttattct tgatattggt gggggaacaa cagaaatggc tgttatttca
     7021 cttggcggac tggtgctcac aaaaagttta cgtgttgctg gcaatgaaat ggatgaggcg
     7081 atttccaatt atctgaagaa gaaatataat cttctcattg gtgaaaagac ggcggaagac
     7141 atcaaaatca atatcggttc cgcattttca cttgaagagg aactgacaat ggaagttcat
     7201 ggaagagatc ttgttgaagg ccttccgaaa atgatcagga tcagggcaga agaaataaga
     7261 gaagcattgc aggaaaccct gatggctatt gttggtgctg taagggacat tcttgaagaa
     7321 acaccaccag aactttcaag tgatcttgtt gaaagaggat tggttattgc gggcggaggt
     7381 gcgcttttac gaaatattga taaacttata tctcaggaaa caaaacttcc cgtagttatc
     7441 gcagagaatc cattgcttgc cgttgtgacc ggtgctggca agatgctgga ggaagatcgt
     7501 tatctgaaga tgtttccttc cgaataggcg gctaaatgtt gtggagattc cgtcgggaaa
     7561 ttatctttat cattctcatc atgctgggcg catggcttat cagacacagt gtgtctttca
     7621 gaatgttttt tccaaaacat acggtgtctt ctggaaattt ccagcaggat gttgagagta
     7681 tcattaatga gaatgaacag ttgaaaaaat tacttggctt gaaacagtca ggaagtttta
     7741 caaaaattat ctatgccagt gtgaaaacaa tttcaccatg ggtttttccc tccagtattg
     7801 tttttgataa gggcactgag gatggtgtga aagagggtat gtctgtaata agttccagcg
     7861 gttctctgat agggagagta atctccgtga ataaaaacaa ttcaacggga attacattgt
     7921 atcatcctga tagcaaggta agtgtcatgg ttttatcaac aggagaactt gcggtcattg
     7981 aaggagtttc tgtggcgtca ttatacccgt atcttagaat aaaatttttg cctccggaat
     8041 gcaaagcttc tgccggtgat attgttgaga caagcggtct cacaagatta tttccgcatc
     8101 atatcagagt tggaagaata gttaaaatca ataattcaag ttcagaacct attacacacg
     8161 gcgttgtggt tccttttttt attaatgaaa atattaaaag ggttgcactg gttgaatagc
     8221 agagaattct ggttgttact aattattcct tctgtgtgtc agtttttact gccacacctg
     8281 tggattgatt gttttcttct tgtattattt tatgtcgctt tcaccaaaaa aatatttcca
     8341 ttttttttaa tggttttttt gtggagcata atattttcaa tcattactgg tgataatcca
     8401 ggtagtgaaa tgctggcgct tgggactatc tggtatttgt tctcttcttt caagattgat
     8461 actgatatca ccaggattgt ttcagcggcg atcagttgtt tgttatatct tattgtgaaa
     8521 ttctgccttg tttcgggtgg ttgtatatgg aatatttcaa tgatgattct tttttcattc
     8581 tggtttataa tgctgcatat ggcaatatgt ttcattttta ttataatgag gtataatctt
     8641 cttaaagggt a
//
LOCUS       DAORYU010000035         8643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_5950_length_8643_cov_5.373777, whole genome shotgun sequence.
ACCESSION   DAORYU010000035 DAORYU010000000
VERSION     DAORYU010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8643
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5950_length_8643_cov_5.373777"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..2667)
                     /gene="carB"
                     /locus_tag="PLW07_02015"
                     /pseudo
     CDS             complement(1..2667)
                     /gene="carB"
                     /locus_tag="PLW07_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018048726.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
     gene            complement(2645..3736)
                     /gene="carA"
                     /locus_tag="PLW07_02020"
     CDS             complement(2645..3736)
                     /gene="carA"
                     /locus_tag="PLW07_02020"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013010847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HOL49331.1"
                     /translation="MKGYLVIEDGTIYAGETFGSQSERFGELIFNTSMTGYQEILYDP
                     SYKGQIVVMTYPLIGNYGVNSQDIESYKIHLEGFVVREKSKIYSNWRAEKSLDQIFNE
                     HGITGIEGVDTRKITELLRDKGAQKAGIFVGEQDKRQMLEKVKKSLPIVGRDMVKYVI
                     KNQPFTWNNQGKYKVVVIDCGVKYNTLRLLAEKNLQVVVYPASAPYQEIDREKPDGIL
                     ISNGPGDPEPLTYVVNTVSNLVEKYPMFGICLGQQILGQVFGGKTYKLKFGHHGANHP
                     VKDLKTGRISITVQNHGFCVDINSIKDPDIKITHINLNDQTLEGIQHKKLPVFSVQFH
                     PEDAPGPQDARYLLEKFYMMIEHGKKNRH"
     gene            complement(3777..5954)
                     /gene="ileS"
                     /locus_tag="PLW07_02025"
     CDS             complement(3777..5954)
                     /gene="ileS"
                     /locus_tag="PLW07_02025"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOL49332.1"
                     /translation="MYWCWHCQTALAEAEVEYWDETSPSVYVKFPVKTFSAGQVPDDS
                     FFLIWTTTPWTLPANVAIAVHPQLDYCLIKTQAGHFVLATTLVDTVCEKLNWTCEKIA
                     TFKGKHLENTVCNHPFVERDSPVVLADYVSDQEGTGCVHTATGHGEEDYQTGLKYNLP
                     VISPVDERGRFTDDVPEWKGLLVFEADPIIVDSLTKKSIILHSENYQHSYPHCWRCKK
                     PVIFRATKQWFLKIDHQNLRDVLQGEIQKTKWIPEEGLSRISSMVALRPDWCLSRQRL
                     WGVALPVFYCERCGTAICNQETINKVASLVEKSGSDVWFEKPVEELLPENFTCPGCKQ
                     NGPFVKEKDILDVWFDSGVSHLAVLKNQPHLKWPCDLYLEGSDQHRGWFQTSLITSCA
                     IEKHAPFKTVLTHGFVVDAEGRKMSKSLGNVITPDQIINKYGADILRLWVVSENYQQD
                     MRISQKIIDHLVIVYRTFRNTMRFMLGNLSDYDSTFQIQDSELLEVDRWAMENFHRLV
                     NVVTQYYQDCLFHKGINEIFDFCNIDMSSFYLDYLKDRLYTHGKNSTSRKAAQKVLYE
                     ILNGLIVMLAPVLSFTCEEAYSFMPEKKQESVFLEQWPETKEPDNLLLERWQKFFEFR
                     KLILKQIEQQRAAGNIGSSLEASIFVKADADYQQFLESFHPYLTNLLMIAEIHIENGD
                     NLAVTAEKTGLPKCARCWIHHKTVGRSNQHPQLCEKCLQVVSS"
     gene            complement(5870..6514)
                     /locus_tag="PLW07_02030"
     CDS             complement(5870..6514)
                     /locus_tag="PLW07_02030"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOL49333.1"
                     /translation="MSENNYSETVLLPKTGFPMKADLAKREPEFLEKWKHLDIYKKLL
                     EKNKDNQSFVLHDGPPYANGHIHIGTALNKILKDTVVKFKSLQGFFSPYVPGWDCHGM
                     PIEHQVFQQMKLKDKNAVDIVSFRKKAHDYAMKFMAIQRDEFKRLGVFGDWDHPYLTL
                     NPLYEKTIVESFGQLFTVDTSFKDLSQYTGAGTVRQPLLKQRLNTGMKQAHQCM"
     gene            complement(6516..6998)
                     /locus_tag="PLW07_02035"
     CDS             complement(6516..6998)
                     /locus_tag="PLW07_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HOL49334.1"
                     /translation="MAKILIVYYSRTGNTEKMAQEIKKGVLEENVEVVVKKVEETEPE
                     ELLNYDGIIAGSPTYFGGPAWEIKKLFDESVKFYGKMVGKVGAAFTSSANIAGGNETT
                     ILSIISMMLIHGMIVQGTTEGGHYGPVAIGGPDEKTLKECRNSGKRVAILVKKLKGEK
                     "
     gene            complement(7065..>8643)
                     /locus_tag="PLW07_02040"
     CDS             complement(7065..>8643)
                     /locus_tag="PLW07_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49335.1"
                     /translation="DPLWKKFAIFRSQSWAEFMKDASNAAKQINENAVMFLNGGHFSA
                     SNIMLGYDAGRMEQFQTFTGAEDFFHCTDLYRSPYTSLNLSRFLSAGKNPSVVFTHHT
                     LSTWHYVPLPEAELSTALAQATAGGSNTWFAIFYPAIEKTKKYATGGLKTTGSLIQMA
                     EKYIAKSKSCAEAGVMISNRTLYFYISSHRGLCYQTGSGREQGLIMDVGKEGQTADIN
                     EMRKISEEILNNENHGCLDLCNFAHIPVRVIWDEYLDEKHLSEIKLLILPNTACLSEY
                     HIRSIMNFVHNGGVLLSDFESGMYDSEGNNVLRKHWLDFLGIKQICGVFRPSRIEEYM
                     MLTENIGNIERDVLIPRPMNALAIHPTEDARVLAKFLNPINLTYRQPQGISNYPALIV
                     TERGKGSVVYFPSAMFESFNRFHIDFHKKLVHSLIKMFISGGLQIETDAPGSLAIELR
                     KNEKFQILHLVNVTSDMKRPMDRIIPLHDIFVSVKTDRKKALSLSSGKSLPATFQKGK
                     LSFTVPLIKEYEMILLS"
BASE COUNT         2359 a         1854 c         1537 g         2893 t
ORIGIN      
        1 taaatggaaa cacagattct ttgacagcaa aataagtaag atgaacttcc tttgtaaatt
       61 gcaatttttt taaactcata cccatcataa tttttgttgc aagctttgca agagggattc
      121 ctgtgacctt gcttacaaac ggtactgttc ttgaagccct gggatttacc tcaagaatat
      181 aaatattatc atccttcaca gcaaattgaa tgttcaatag ccccacaaca ttcagagata
      241 aggcaagttt ttttgttgcc tgttttattt cattgattat tttttccttt aaagcatatg
      301 gtggaagtac cattgcgctg tctccagagt gaatgcctgc ctgttccaca tgctccatca
      361 cgccgcctat cacgcatgtt tttccatcgc atattgcatc aacatccacc tcagcggcat
      421 cctcaagaaa tttgtctatc aacacaggat gttctggtga aacctcaatg gctttttcca
      481 taaacgtttg aagcatgtca tcatcataga caacttgcat cgcccttcca cccagtacat
      541 acgatggcct tacaagcaca gggtatccaa gttcatgagc aatttttaat gcatctcttt
      601 tgtttctggc agtaccactt gcgggttgca taagatccag cgcatcaagg agttttttga
      661 atttttttct atcttctgca atatctatgg cgtccgggct tgtaccaaga atttttacgc
      721 ctgctttttt caaagtaatt gcaagtttca aaggagtttg ccctccgaac tgcagtatga
      781 caccataagg tttttctttc tcaataatat tcaaaacatc ttcaaatatc agcggttcaa
      841 agtagagttt atcagaagta tcataatctg tgctgacagt ttcaggattg cagtttacca
      901 taatgctttc ataaccaatt tctttcaggg cgaacgaagc atgaacacag caatagtcaa
      961 actctattcc ctgaccaatc ctgtttggtc ctgcaccaag tattacaacc tttttcctca
     1021 tatgattttt attatttctg gatgctcgta agttgaataa tagtacggtg tataagcgac
     1081 aaattcgccc gcgcatgtat caacgcattt atattcaacg tctatcccgt atttttttct
     1141 taaggttctt atttttttct cactgcaacg cacaatttta gcaatatgat tgtctgaaaa
     1201 tccaaattct tttgcctttt tcaacatttc ataattaatt gttttacctt tttgtttctc
     1261 aatctttttt tcaaattcaa caatttcatt gatattcgag agaaaccatc tgtcaatttt
     1321 tgttagatga taaatttcat caatggacca gttctgtttc agggcttgtt taatataaaa
     1381 cacccttttt gaatttggaa ttttcaatcc tccctgaatg tcaagcggca acgaaatatt
     1441 ttcaaatcca atgaacttct cttccatgct tctcagcgcc ttctggaacg cttccttaaa
     1501 agtcctgcca atagccatca cttcaccaac tgatttcatt gaagtggtca atacatcaga
     1561 tgctcctgga aatttttcaa aattgaactt tggaattttc acaacacaat agtcaatcgc
     1621 aggttcaaaa caggcaggtg tcttttttgt tatatcattg ggtatctcat caagggtata
     1681 accaatggca agttttgtgg cgatttttgc gatgggaaaa cctgttgctt ttgaagcaag
     1741 ggctgaactt cttgaaacac ggggattcat ttcaataaca accatgtctc cgttttcagg
     1801 attgagcgca aactgaatat ttgaaccacc tgtttcaacg ccaatttctc tgataatgtc
     1861 aatggatgca tttctcattt tttgatattc tttgtcagtc aaggtctgca caggcgcaac
     1921 agtgatactg tctcctgtgt gaactcccat cgggtcaaca ttttctatag aacagataat
     1981 caccacattg tccttcaaat ccctcatgac ttctaattcg tattctttcc agccaaggac
     2041 tgattgttca acaagaattt ctccaatgaa acttgctttt aatccaacat ctgcaaattc
     2101 ttcaaactct tcaatgttat atgccacaga gccaccggtt ccaccaagtg tataggcagg
     2161 tctgatgata gcagggaaac caattttttt taccacttcc attgcttctt tcaaactgcg
     2221 ggcgataccg ctttcaggca ccttcagtcc gattttttcc attgatttct tgaaaagttc
     2281 cctgccttct gcctttttta ttgcctgata atttgctcca atcattttga cattgttttt
     2341 ttcaagcact ccgagttcat aaagttttgt ggcaaggttt aatgcggtct gtccgccaag
     2401 agttggaagt aaggcgtcgg gcttttcttt ttctatgatt ttttcaagta tttcagggat
     2461 aagaggttca agataaattt tatctgccat ttcagggtct gtcataatgg ttgcgggatt
     2521 actgttgaca aggacaacct tatagccctc ttctttcaat gccttgcatg cttgcgaacc
     2581 cgaataatca aattcgcatg cctgtcctat tacaatagga cctgaaccta tgagcaaaat
     2641 tttctcaatg tctgttcttt ttcccatgtt caatcatcat ataaaatttt tcaagcaaat
     2701 atcgagcgtc ctgcgggcca ggtgcatctt ctggatgaaa ttggacgctg aatacaggta
     2761 gtttcttatg ctggatacct tcaagtgtct ggtcattgag attgatatgt gtaattttaa
     2821 tatcagggtc tttgattgaa ttgatgtcaa cacagaatcc atgattctgg actgttattg
     2881 aaatccttcc tgttttcaaa tcttttacag gatggtttgc tccgtgatgg ccgaatttta
     2941 acttgtaggt tttaccgcca aaaacctgcc caagaatctg ctgaccaaga cagattccaa
     3001 acattggata tttttcaaca aggtttgaaa cagtatttac tacatatgtt aatggttcag
     3061 ggtctcctgg tccattggaa attaaaatcc catcaggttt ttctctgtct atttcttgat
     3121 acggagcact ggcaggatac acaacaacct gtaagttttt ttctgcaagc aatctcaatg
     3181 tgttgtactt caccccgcag tcaatcacaa caaccttata ttttccctga ttgttccatg
     3241 taaaaggttg atttttgatg acatacttca ccatatccct tccaacaata ggcagagatt
     3301 tttttacctt ttcaagcatc tgtcgtttgt cctgctctcc aacaaatata cctgctttct
     3361 gggcaccttt atctctgagc agttctgtga tttttctcgt gtcaacacct tctatgccag
     3421 taattccatg ttcattgaaa atctggtcaa gggatttctc agccctccag tttgagtata
     3481 tcttgctttt ttccctgaca acaaaccctt caagatgaat tttgtatgat tctatgtcct
     3541 ggctgtttac tccataattt cctatcaggg gatatgtcat tacgacgatc tgtcctttat
     3601 acgaagggtc atacaggatt tcctggtaac cggtcatact tgtattgaaa atcaattcac
     3661 caaatcgttc actctggctg ccaaaggttt cgccggcata tatcgttcca tcttcaatga
     3721 caaggtatcc tttcattttt tcagttctcg ccaggaatta aatcagtaaa tttctttcaa
     3781 ctactcacaa cctgaaggca tttttcacag agttgaggat gctggtttga acgacctaca
     3841 gttttgtggt gtatccagca ccttgcgcac tttggaagac ctgttttctc tgctgtcact
     3901 gccaggttat ctccattttc aatatggatt tctgctatca tcaacaggtt tgtcagatat
     3961 ggatgaaaac tctcaagaaa ttgctggtag tcagcatctg ctttcacaaa gattgatgcc
     4021 tcaaggctgc ttccaatgtt tcctgctgcc ctctgctgtt caatttgttt gagaatcaat
     4081 ttcctgaatt caaaaaattt ctgccatcgt tcaagcaaaa gattatcagg ttcttttgtt
     4141 tcaggccatt gttcaagaaa cacgctttcc tgtttctttt ccggcataaa agaatatgct
     4201 tcctcacatg taaaagaaag aaccggcgca agcatgacaa tcagcccatt taatatttca
     4261 taaagtacct tctgggctgc tttccttgat gtggaatttt ttccgtgtgt gtataatcta
     4321 tccttgaggt agtccagata aaacgaagac atgtcaatat tacagaaatc aaagatttca
     4381 ttgattccct tgtgaaacag acaatcctgg taatattgtg tcacgacatt gaccagacga
     4441 tgaaagtttt ccattgccca tctgtcaacc tcaagaagtt cactatcctg aatctgaaat
     4501 gttgaatcat aatcagaaag gttaccaagc atgaacctca tcgtattgcg aaatgttcta
     4561 tacacaatga caagatggtc aataattttc tgggaaattc tcatatcctg ctggtaattt
     4621 tcactcacaa cccataacct caaaatgtca gcgccatatt tattgataat ctggtctgga
     4681 gtaatcacat ttccaaggga ttttgacatc tttcttcctt cagcatcaac aacaaatcca
     4741 tgcgtgagca ctgttttgaa aggagcgtgt ttttcaatgg cgcaggaagt gatgagagat
     4801 gtctgaaacc atcccctgtg ctggtcgctt ccttcaagat acaaatcaca tggccatttc
     4861 agatgtggct ggtttttcaa cacagcaaga tgactgaccc ctgaatcaaa ccagacatca
     4921 agaatatctt tttcttttac aaatggccca ttctgtttgc aaccggggca ggtgaaattt
     4981 tcaggcagga gttcctcaac tggtttttca aaccagacat ctgaaccaga tttttcaacc
     5041 agagaagcaa ccttattgat tgtttcctga ttgcaaattg cagtgccaca tcgttcacag
     5101 taaaaaactg gaagagccac gccccacaat ctctgtcttg acaggcacca gtctggtctt
     5161 aaagcaacca tgcttgatat tcttgataaa ccttcttctg gaatccattt tgttttttgg
     5221 atttcaccct gcaatacatc tcgtaaattc tgatggtcaa tttttaaaaa ccattgcttt
     5281 gttgccctga aaatcactgg ttttttacat cgccagcaat gtggatatga atgctggtaa
     5341 ttttcactgt gcagaataat actttttttc gttaaactgt caacgatgat agggtctgct
     5401 tcaaacacaa gcaaaccctt ccattctgga acatcgtctg taaatcttcc tctttcatcc
     5461 acaggagaaa taacaggaag attgtatttc aagccagttt gatagtcctc ttcaccgtgt
     5521 cctgttgcag tatgcacgca acctgtacct tcctggtcag agacataatc agcaagcacc
     5581 acagggctat ccctctccac aaatggatgg ttgcacactg tattttcaag atgcttgcct
     5641 ttgaacgttg caattttttc gcatgtccag ttcaatttct cacagacagt atcaacaagg
     5701 gttgttgcga gaacaaaatg acctgcctgt gttttgatca ggcagtagtc aagttgagga
     5761 tgaacagcaa tcgctacatt tgcgggtagt gtccatggtg ttgttgtcca gatcaggaaa
     5821 aaactatcat caggtacctg ccctgcagaa aatgttttca caggaaattt tacatacact
     5881 gatgggcttg tttcatccca gtattcaacc tctgcttcag caagggctgt ctgacagtgc
     5941 cagcaccagt atattggctt aaatctttga atgatgtatc tactgtaaag agttgtccga
     6001 aactttcaac aatcgttttt tcatacaaag ggtttaacgt aagatatgga tggtcccagt
     6061 ctccaaacac gccaagtcgc ttgaattcat ccctttgaat tgccataaat ttcattgcgt
     6121 aatcatgcgc cttttttctg aaagaaacga tgtcaacagc atttttgtcc ttcaatttca
     6181 tctgctggaa aacctggtgt tcaatcggca ttccatgaca gtcccagcca gggacataag
     6241 gcgagaaaaa tccctgcaat gatttgaatt ttacaacagt atctttgaga atcttgttta
     6301 atgctgtgcc aatgtggatg tgtccattgg catacggggg accatcatga agaacaaaac
     6361 tttgattatc tttatttttt tcaagaagtt ttttgtaaat atcaagatgt ttccattttt
     6421 caagaaattc tggttccctt tttgcaaggt ctgctttcat gggaaaacca gtttttggaa
     6481 gcagaacagt ttcagaataa ttgttttcag acattttatt tttcaccttt cagttttttt
     6541 accagtatcg ctaccctttt tcctgaattt ctgcattctt ttaatgtttt ttcatcaggt
     6601 ccaccgatag ccacaggtcc ataatgtcca ccctcggttg ttccctggac aatcattcca
     6661 tgaatcaaca tcatgctgat gatggaaaga atggttgttt cattaccacc agcaatattg
     6721 gctgatgaag taaatgcagc gcccacttta ccaaccattt ttccataaaa ttttactgat
     6781 tcgtcaaaca gtttttttat ttcccaggca ggtccaccga aatatgttgg tgaaccagcg
     6841 atgatgccgt cgtaattcaa aagttcctct ggttctgttt cttcgacttt tttcacaaca
     6901 acctcaacat tctcttcaag gactcccttt tttatttcct gtgccatttt ttcagtgtta
     6961 cctgttctgg aataatagac aataagaatt tttgccatat gccacctcct gttagcaaaa
     7021 acattgagat atattataca atcaaaacgc cagggaatga aacattatga aagcaggatc
     7081 atttcgtatt cctttatcag tggtactgtg aatgagagtt ttcctttctg aaatgttgct
     7141 ggtagcgatt tccctgatga tagagaaagt gcctttttcc tgtctgtttt tactgaaaca
     7201 aaaatatcat gaagcggaat aattctgtcc ataggtcttt tcatatcaga tgttacattg
     7261 acaagatgaa gaatctgaaa tttttcattt tttcttaatt ctatcgctaa acttccaggt
     7321 gcatcagttt caatctgcag tccccctgat atgaacatct ttatcaatga atgcaccagt
     7381 tttttatgaa aatcaatatg aaaccgattg aaactttcaa acattgctga aggaaaataa
     7441 acaacactgc cttttcctct ttcagttacg atgagcgcag gatagttgct gataccctgc
     7501 ggttgtctgt atgttagatt tatagggttg agaaactttg caagtactct ggcatcctct
     7561 gtggggtgta tcgcaagggc gttcattggc ctcggaatga ggacgtccct ttctatattt
     7621 ccaatgtttt cagttaacat catatactcc tcaattcttg atggtctgaa aacaccgcag
     7681 atttgtttta ttccaagaaa atcaagccag tgctttctga gtacattatt cccttcgctg
     7741 tcatacatgc ctgattcgaa atcagaaagc agtactccgc cattgtgtac aaaattcatt
     7801 attgacctga tgtggtattc agaaagacat gcggtattcg gaagaattaa aagttttatt
     7861 tcagacagat gtttttcatc gagatactca tcccatatga cccgtactgg tatatgcgca
     7921 aaattacaca ggtcaagaca gccgtgattt tcgttattca aaatttcctc agagattttt
     7981 ctcatttcat tgatatctgc tgtctgaccc tcttttccta catccatgat aagaccctgt
     8041 tctctgccag agccagtctg gtagcaaagt cctctgtggg aagaaatata gaaataaagg
     8101 gttctatttg aaatcattac gccggcttct gcacatgact tgcttttagc aatatatttt
     8161 tcagccattt gaattaaaga acctgttgtt ttaaggccgc ctgtggcata ttttttcgtt
     8221 ttttcaatcg ctgggtaaaa tatcgcaaac catgtgtttg aaccgcctgc tgttgcctgt
     8281 gccagcgctg ttgagagttc tgcctcaggc aagggtacat agtgccatgt tgaaagagta
     8341 tgatgcgtaa ataccactga agggtttttc cctgcagaaa gaaatcttga aaggtttaaa
     8401 gatgtatatg gcgaacgata caaatctgta cagtggaaaa agtcctcagc acctgtgaaa
     8461 gtttgaaact gttccatcct tcctgcgtca taaccgagca taatgtttga agcgctgaaa
     8521 tgaccgccat tgagaaacat tacagcattt tcgtttattt gttttgctgc gtttgaggca
     8581 tccttcatga attctgccca gctctgtgag cgaaaaatag caaatttttt ccagagcggg
     8641 tct
//
LOCUS       DAORYU010000036         8243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_6267_length_8243_cov_4.615169, whole genome shotgun sequence.
ACCESSION   DAORYU010000036 DAORYU010000000
VERSION     DAORYU010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8243
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6267_length_8243_cov_4.615169"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1472)
                     /locus_tag="PLW07_02045"
     CDS             complement(<1..1472)
                     /locus_tag="PLW07_02045"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOL49336.1"
                     /translation="MKKSIAIIICIICTLAFCADLKQADTLFKAEKWQEARKEYQSVL
                     QELKGKEMAEVLYKIGYTFEREGGKFDEAIDAYRKATLVEEADDVLKARSYLRMGYIL
                     KLQRKFQQAIEEFTRIEQLRNFPPEILGEALLYKAWSYNNLNQDALAFETFKKIVENE
                     NIPAINRATALLNIAGKLQKQKNYKEAIKEYQKVIDLKGATKEQKDEANNYLIECKNL
                     LEGPKPFYIRPYVTRVSPDSAEIYWISQLTDEKAKFILKSQDQTIELNPEVSPIRTTE
                     CFLFCVKVTNLKPGVLYRYEVICEKEKTEGSFKTPSETNKNVIFCVITDTQLNMDVHM
                     KMAELISKENPDFILHTGDLTDKGSSWSRWKAELFDPGYQYFKMAAFYPAIGNHDGGP
                     FFRLLFGMKQGKNYYDVTYGNTQIIVIDSYWAGGPGSKSRQQQLNWLEQVLSQSKATW
                     KIVGLHVPMVSAKTGDRPFGQQDFLPVLEKYGADIVLAGHV"
     gene            complement(1475..2677)
                     /locus_tag="PLW07_02050"
     CDS             complement(1475..2677)
                     /locus_tag="PLW07_02050"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOL49337.1"
                     /translation="MLRQATEKDRDAIMRIVKEAFNYNAEKMKTVQQMNLSNWHVYQY
                     DNQIIGCLRIARDILRIGKAKIVKGEVGYVSILPEYQGKNHGTLMMQSAVSWMNGNDF
                     DISRLGGRVRFYSRFGYIRFPRRYIEIFVSPDTGIITNQTHHEQLPGQIQIFNAEKDK
                     KELETFISTHNLETTGQPEYFGKYDEGKILLYRENNKIRGVIGYRTSDKEHSNFEAKI
                     EIDMLVYDRDCQEAFKHLVTYIHNEAFRSNINRITCRIPFSPEIVETLSNTEIFFNLV
                     ETYGGRSSNMLQVINFNSLVEKLIPEFQHRLQMAGIETEKTIKLTVSNKGSIYLKIIG
                     NKMKIVENHSYDIHFEFSQLHFLQLILGLISYQQLHYTYRQSQDTETYLLGTVLFPQT
                     VVFSGCWG"
     gene            complement(2658..4121)
                     /locus_tag="PLW07_02055"
     CDS             complement(2658..4121)
                     /locus_tag="PLW07_02055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOL49338.1"
                     /translation="MMKTKIGFAGFLVVLLFTVIIISGLKHHISCIRFSYPIDYREGV
                     VQYWVNSFVNKQPLYPEIQQFPPYVHNPYTPLYIVLTGFLDRLIPEHLVFLAGRLISF
                     LCLILSCFLIFLIARRYGSSIAGFFAAGMFFCNPVAINYGSIETVDMMALCLGLSGIY
                     LAIRKKDSPGTLSGILCAAAILTKPVFVLPAISIFISMLYNKTVRKSYFIMSFIGCLL
                     LSFLILFLRNKSMFNHLVVLNMLPLSVIHFFTLFSVVASKHVILFVLLFVFVFTYKDK
                     KNPFYWYCVLSPIAIVFSAKIGAEANYFLEIIALSSIATGILLTTIDTSLKHIALPVC
                     IVQLFLFLPFKPAPVFTRTYGQEIPASLSTEPDKILKEAGDLITGEILSCSDPILSED
                     IGWLVLCQRNVYIEPYQFSQLAKHRRWDETFILDMIKEKQFNLIVMNAGSFEQDCVAF
                     TKNMLKAIKEHYTIKRVIGNFYVHEPVFWRDNAQTGN"
     gene            complement(4118..5674)
                     /locus_tag="PLW07_02060"
     CDS             complement(4118..5674)
                     /locus_tag="PLW07_02060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOL49339.1"
                     /translation="MKKHLTALLFISLGLLWLYFVLFHGFDDDEFQHCHNAFLIWKGF
                     VPYRDFFEHHIPLYHILFSGLFAFDTGRWTIFLFRIASVIASAGVFVLIGKYLKKTYD
                     SATAIISLTLLACVPMFLIKMTEARPESIAVFVFALTMIFVFSHHFDRNVFFTGILSG
                     LMVCFSQKYFFVYIGILVACFIMHGRKQTVFFIAGSFSMFFVLILYLVSKGILPDFIN
                     STLLMNIKWKYRFSPAGYFYQAFQTAGFLIACGIAGIIGDFFISGNRKKATSLFAIML
                     GCLLAIMIVPVPYRQVFLPFFVVLSIGSGWFFFHFLKIFPSQKLKGIAGVIIVVCAMS
                     GAIPVIVQEAGKKNSTDLATMQKADEISPGGTFFDGRCLLFYRMHTGYFAFIHHEILE
                     MLDLQQYANQTIQAIKANNFPLVIYDYRVKQMPDDIQSFIQTHYCPTGFHDIYMPGVI
                     IDRAQFIDGKADFEINVSGWYRINLKGEHLLIDGKPVSDRDMIFLKSGIHAAEISGFV
                     ESVTVILEKR"
     gene            complement(5671..7968)
                     /locus_tag="PLW07_02065"
     CDS             complement(5671..7968)
                     /locus_tag="PLW07_02065"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOL49340.1"
                     /translation="MSTLFKVALFCLAFGYQLFAVEFPLKDVCYNACMEISGNWLFRF
                     DGSDIGIKERWYDKNFDRSGWEKINVPGIWNKKGGEVSYPCPETVGWYYRNVTIPRTW
                     EEEVLMVFFGSMYTTDVWVNGNYAGIHRGGYTPFMVDITKFIEPGEKNTIVVRVDNRR
                     GKTIPATGMGWHPYGGIYREVHLIHRGFVHLESIASSTQISGNQTVFKFTADVVNNSK
                     TNFSGNVTLQLKDKQKTAGFRTTYVQIPAHSRKNISFSIPVQNAKLWSPENPYLYIVN
                     LLLPYRIPQKVEFQTGLRQFIAKDGKLYLNGKRIWLQGFGNHEEYPGYGSLANEKMMK
                     KDLMLMKNVYNVNTVRTGHYPFHPVFFELCDKLGIIVHTEIPAWQVDKNFMQSDEAWN
                     LWLKCQLEEMVSSFRNHPCIAFWGLSNELYNVPLYHKKAYDFVRSLDTDRFITIVCAS
                     TADLESNKIADIIARNFHYGWYHSQSVYALRDNLPVVLRASEGKPIWVAEIGALANYG
                     KYSGGYGDQSRGSETYQDKVVRYGVQYCATQTDQICGVSVWTLSDFHGRNQFLPHGIL
                     DENRKPKILGYTICNLFNGNIRVYICEQDTMIKQGVYRASLRYFNPEEKRFQNLVAKW
                     CIIKGQNKIASGSFTFSVFGTRQGEIGNIEFQTPEQPGLYSLWVELYDSKGNWLYTNS
                     GFFDVKEVQIPGVLKVNTDKKYQNLKMMYQGIQVPVYDDIGLVLPFEEGKYQIGFKAD
                     GFKDISHEIEIVSGKATELTVRFEK"
     gene            complement(7971..>8243)
                     /locus_tag="PLW07_02070"
     CDS             complement(7971..>8243)
                     /locus_tag="PLW07_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOL49341.1"
                     /translation="GLCNILYGTKISDMETGYKVFSKKVLNQMNIVSDGFEFEPEFTI
                     LAAKHKFRITQVGISYYPRPYAQGKKIKFKDGLLAILTIFKMKFRR"
BASE COUNT         2516 a         1594 c         1428 g         2705 t
ORIGIN      
        1 acatgtcctg caagaacaat atcagcgccg tatttttcaa gaactggcag gaaatcctgc
       61 tggccaaagg gtctatctcc tgtttttgct gataccattg gaacatgaag tccaacaatt
      121 ttccaggttg ccttactctg ggacagtact tgttcaagcc agtttaactg ttgctgcctg
      181 gatttacttc ctggaccacc tgcccagtat gaatcaatga caataatctg cgtgtttcca
      241 tatgtaacgt cgtaataatt ttttccctgt ttcattccaa aaagcaagcg aaaaaaagga
      301 cctccatcat ggtttccaat tgccggataa aatgctgcca ttttgaaata ctgatagccc
      361 ggatcaaaca gttctgcctt ccatctggac caggaactgc ctttatcagt caggtctcct
      421 gtgtgaagaa taaaatcagg attttcttta ctgatcagtt ctgccatttt catgtgaaca
      481 tccatattaa gctgggtgtc tgtaatcacg cagaagatga catttttatt tgtttcagat
      541 ggtgttttga aggagccctc tgttttttcc ttttcgcaaa tcacttcata cctgtagagt
      601 actccaggtt taagattggt gacttttaca cagaaaagaa aacattctgt tgtgcgaatt
      661 ggcgatactt ctgggttaag ttcaattgtc tggtcctgac ttttcaggat aaattttgcc
      721 ttttcatcag ttaactgtga tatccagtat atttctgctg aatcaggaga tactcttgtg
      781 acatatggcc ttatgtaaaa tggttttgga ccttcaagca gattcttgca ttcaataaga
      841 taattgtttg cttcgtcttt ctgttctttt gttgcaccct tcaggtcaat gaccttttga
      901 tactctttta ttgcttcttt gtagtttttc tgcttctgaa gtttgccagc aatgttcaat
      961 aatgcagttg ctctattaat tgcagggata ttttcgtttt ctacaatctt tttgaaagtt
     1021 tcaaaggcaa gggcatcttg attgaggtta ttatatgacc atgctttgta gagaagtgct
     1081 tcaccaagta tttctggcgg aaaatttctc aattgttcaa ttcttgtgaa ttcttcaatt
     1141 gcctgttgaa attttctctg tagtttcaga atatacccca tccttaaata agagcgggct
     1201 tttaatacgt catctgcttc ttccaccagg gttgcttttc tataggcatc tattgcctca
     1261 tcaaattttc caccttctct ttcaaaagtg tatccaattt tgtaaaggac ttctgccatt
     1321 tctttgccct tcaattcttg aagtacagat tggtattctt ttcttgcttc ctgccatttt
     1381 tctgctttga aaagtgtatc agcctgtttt aaatcggcac aaaaagcaag tgtacagata
     1441 atgcaaatta taattgcaat tgattttttc atttttatcc ccagcaacca gaaaagacaa
     1501 ctgtttgtgg aaaaagaact gttccaagaa ggtatgtttc tgtatcctga ctttgacgat
     1561 aagtgtaatg cagttgttga taggaaatta atcccagaat aagttggaga aaatgtaact
     1621 gggaaaactc aaaatggatg tcgtaactat gattttctac tattttcatt ttatttccaa
     1681 tgattttgag atatattgaa cccttattgc ttactgtgag tttaattgtt ttttctgttt
     1741 caatgccagc catttgtaat ctgtgttgaa attcagggat cagtttttca acgagagaat
     1801 taaaattgat aacctgcaac atgtttgaag acctgccgcc atatgtttca accagattga
     1861 aaaagatttc tgtgtttgaa agagtttcca caatttctgg gctgaatggt attctgcacg
     1921 tgatacggtt gatgttactt ctgaaagcct cgttgtggat ataagtcacc aggtgtttga
     1981 atgcttcctg gcaatccctg tcatagacaa gcatatcaat ctcaattttt gcttcaaagt
     2041 tagaatgttc tttgtctgat gttctataac caatgacccc tctaatctta ttattttctc
     2101 tgtataacaa aatttttcct tcatcatatt ttccgaaata ttcaggctga ccggttgttt
     2161 caagattatg agttgagatg aatgtttcaa gttctttttt atccttttct gcattaaaaa
     2221 tttggatttg cccgggaagt tgttcatgat gggtttgatt tgttatgatt ccagtatctg
     2281 gactcacgaa aatttctatg tatcttcttg gaaatctgat atagccaaat cttgaataaa
     2341 accttactct cccaccaagt cgggaaatat cgaaatcatt tccattcatc caggaaacag
     2401 cactttgcat catcagtgta ccgtgatttt tcccctgata ctcaggaaga atactgacat
     2461 aacccacttc tcccttaaca atttttgctt ttccaattct taaaatatct ctagcgattc
     2521 ttaaacaacc gattatctgg ttatcgtatt gatagacatg ccagtttgat aaattcattt
     2581 gttgaacagt tttcattttt tccgcattat aattaaatgc ttctttcact atccgcatga
     2641 tagcatctct atctttttca gttgcctgtc tgagcattgt ccctccagaa tactggttca
     2701 tgtacataaa aattgcctat cacccttttg atggtgtaat gttcttttat tgcttttaac
     2761 atgttcttgg taaatgcaac gcaatcttgt tcaaaacttc cagcattcat tacaattaga
     2821 ttgaattgct tttcttttat catgtcaaga atgaaagttt catcccatct tctgtgtttt
     2881 gcaagttgag aaaactgata tggttcaatg taaacatttc tctgacatag taccagccat
     2941 ccgatgtctt ctgaaagtat ggggtctgaa catgataata tttcaccagt gattaagtcg
     3001 cctgcttcct tcaaaatttt atcaggttct gtgcttaatg acgcaggaat ttcctgtcca
     3061 taggttcttg taaatactgg cgctggtttg aatggcaaaa aaagaaatag ttgtacaata
     3121 catacaggca aagcaatgtg tttcaatgat gtgtcaatgg ttgtaagtaa aatacctgtc
     3181 gcaatagatg aaagtgctat gatttcaagg aaatagttgg cttcagcgcc aattttcgct
     3241 gaaaacacga tggcaatcgg agacagtaca cagtaccagt aaaagggatt ttttttatct
     3301 ttatatgtga aaacaaatac aaacaacaaa acaaacaaaa tgacatgttt tgatgcgacc
     3361 actgagaaaa gggtgaaaaa gtgaatgaca cttaatggta gcatgttcaa tacaacaaga
     3421 tggttgaaca tggatttgtt tctgagaaag agaattaaaa aggacagcag aagacatccg
     3481 ataaatgaca ttatgaaata acttttcctg acagttttgt tataaagcat tgaaatgaaa
     3541 atactgatgg caggcagaac aaacactggt tttgttaaga tggctgctgc acacagaatt
     3601 cctgataaag tacctggaga atcttttttc cttattgcca ggtaaatgcc tgataatcca
     3661 agacacaatg ccatcatatc aacggtttca atactgccat agtttattgc gacagggttg
     3721 cagaaaaaca ttcctgcagc aaaaaaacct gcaatgcttg aaccatatct tctggcaatc
     3781 aaaaaaatca aaaaacagga taatatcaga cataggaaac tgatcaatct tcctgcaaga
     3841 aacaccaaat gttctggtat cagccggtct aaaaacccag taagaacaat gtagagtggg
     3901 gtatagggat tatgcacata tggtggaaat tgttgaattt ctggatacag tggttgttta
     3961 ttaacaaagg aatttaccca gtattgaaca actccttctc tgtaatcaat cggatatgaa
     4021 aatctgatac aactgatgtg atgttttaat ccagatatga tgattacagt aaaaagcaac
     4081 actaccagaa aaccagcaaa tccaattttt gttttcatca tcgtttttca agtatcactg
     4141 ttacgctttc aacaaaacca gatatttctg ctgcatgaat tcctgatttt agaaaaatca
     4201 tatctctgtc agatacaggt tttccatcaa tcaaaagatg ttcacctttt aagtttattc
     4261 tgtaccagcc agacacattg atttcaaaat ctgcctttcc atcaataaac tgagccctgt
     4321 caatgattac ccctggcatg taaatatcat gaaatcctgt gggacaataa tgtgtttgaa
     4381 taaaactttg aatgtcatct ggcatctgtt ttaccctgta atcatagatg accaatggga
     4441 aattattggc ttttattgcc tgaattgtct ggtttgcgta ctgctgaaga tccagcattt
     4501 caagaatttc atgatggata aaagcaaaat atcctgtatg cattctgtaa aataataagc
     4561 atcttccatc aaaaaatgta cctccaggag aaatttcatc tgccttttgc attgtggcaa
     4621 ggtctgtaga attttttttc cctgcttcct gcacaatgac tggtattgca cctgacattg
     4681 cgcatacaac gattattact ccagcaatgc ctttcaattt ctgagatggg aaaattttca
     4741 aaaaatgaaa gaaaaaccag ccactcccaa ttgataagac aacaaaaaaa ggtaaaaata
     4801 cctgcctgta agggacagga acaatcataa ttgcaagtaa acaaccaagc attattgcaa
     4861 acaaagaagt tgcttttttt ctattccctg aaataaaaaa atccccaata atgccagcaa
     4921 taccgcaggc aatcaaaaat cctgctgtct gaaatgcctg ataaaaatat cctgctggtg
     4981 aaaatctgta tttccatttt atattcatca acaatgttga attaatgaaa tcaggcagaa
     5041 tccctttgga tacaagataa agaataagaa caaagaacat tgaaaatgac cctgcgatga
     5101 aaaaaacagt ttgttttctt ccatgcataa tgaagcacgc aacaagaatt ccaatgtaca
     5161 caaaaaagta tttttgagaa aaacacacca tcaaaccaga aaggatacct gtgaaaaata
     5221 cattgcggtc gaaatggtga gaaaagacaa aaatcattgt aagggcaaaa acaaatactg
     5281 cgatgctttc tggtcttgct tctgtcattt taatcaaaaa catcggaaca catgcaagaa
     5341 gggttaaaga aatgattgct gtagcactgt cataagtttt tttcaggtat tttccaatga
     5401 gaacaaaaac gcctgcactt gcaataacac tggcaattct gaataggaaa attgtccacc
     5461 gtccagtatc aaaagcaaaa agccctgaaa ataaaatgtg gtataaagga atatggtgct
     5521 caaaaaagtc cctgtaagga acaaatcctt tccagattaa gaatgcgttg tggcagtgct
     5581 ggaattcatc atcatcaaaa ccatggaaca aaacgaaata aagccataac agccccaaag
     5641 aaataaataa aagtgctgtg agatgttttt tcacttttca aatctgactg ttagttcagt
     5701 tgcttttcct gatacaattt caatctcgtg agaaatatct ttaaagccat ctgctttgaa
     5761 gccaatctgg tatttacctt cttcaaacgg cagtaccagt ccaatgtcat catacactgg
     5821 tacttgaatg ccctgataca tcattttcaa attctgatat tttttgtctg tgttcacctt
     5881 caacacacca ggtatttgta cttctttgac atcaaaaaag ccactgtttg tataaagcca
     5941 gttgcctttt gaatcataaa gttcaaccca taaactgtat agtccaggtt gctctggcgt
     6001 ctgaaattct atgtttccta tttctccctg ccgcgttccg aaaacagaga aggtaaaact
     6061 tcctgaggca attttgtttt gtcctttgat aatgcaccat tttgcaacaa gattttgaaa
     6121 tcttttttct tcaggattga aatatctcaa agaagcacga tagacaccct gtttaatcat
     6181 tgtgtcttgc tcacaaatat atacgcgtat gtttccatta aatagattgc aaattgtata
     6241 tccaaggatt tttggctttc tattttcatc aagaattcca tggggcaaga attgatttct
     6301 tccatgaaaa tctgataaag tccatacact gactccacaa atctggtctg tctgggttgc
     6361 gcaatactga acgccatatc ttaccacttt atcctgatag gtttctgaac cgcgcgactg
     6421 gtctccatat ccacctgaat attttccata attggcaagt gcgccgattt ctgcgaccca
     6481 gattggtttt ccttcagaag cccttaatac aactggtaga ttgtctctaa gggcataaac
     6541 actttgagaa tggtaccagc catagtggaa gtttcttgcg atgatatcag caattttgtt
     6601 tgattcaagg tcagcggttg atgcgcagac gattgtgatg aatctatcag tatcaaggga
     6661 gcggacaaaa tcatatgcct ttttatgata aagagggaca ttgtacagtt catttgagag
     6721 accccagaag gcgatgcatg gatgatttct gaaacttgaa accatttcct caagttgaca
     6781 tttcagccag agattccatg cttcatctga ctgcataaag tttttgtcaa cctgccaggc
     6841 aggtatttca gtatgtacga taatcccaag tttatcgcac aattcaaaaa acactggatg
     6901 gaatggataa tgacctgttc ttacagtgtt gacattgtaa acatttttca tcagcatcag
     6961 gtcttttttc atcatttttt cattcgccag tgaaccatat cctggatatt cctcatgatt
     7021 cccaaaaccc tgaagccata tccgttttcc attgaggtac agttttccat cttttgcaat
     7081 aaattgtcgc aacccagttt gaaactcaac tttttgagga attctatatg gcaggagtag
     7141 atttacaatg taaagatacg gattttcagg agaccagagt ttcgcatttt gaacaggaat
     7201 tgaaaaactg atgttttttc ttgaatgtgc aggaatctga acataagttg ttctgaaacc
     7261 agcagttttt tgtttatctt tgagttgaag tgttacattg cctgaaaaat ttgtttttga
     7321 attgttcaca acatctgccg tgaatttaaa gacagtttga tttccactta tttgggttga
     7381 gcttgcgatt gactcaagat gaacaaatcc tctatgaata agatgaactt ccctgtaaat
     7441 tccaccataa ggatgccatc ccataccagt ggcaggtatt gttttgcccc gtctgttatc
     7501 aacccttact actatggtat ttttttctcc tggctcaatg aattttgtga tgtcaaccat
     7561 aaaaggtgtg tatcctcccc tgtgtattcc agcatagtta ccattaaccc atacatcagt
     7621 ggtatacatt gaaccaaaaa ataccataag cacttcctct tcccatgttc gtgggattgt
     7681 cacattccga tagtaccatc ccacagtttc aggacaggga tatgaaactt caccaccttt
     7741 tttattccat attccaggaa cattgatttt ttcccagcca gacctatcaa aatttttgtc
     7801 ataccatcgt tcttttattc cgatgtcaga accatcaaac ctgaaaagcc agtttcctga
     7861 tatttccatg caagcattat aacaaacatc tttcagtgga aattcaactg cgaaaagttg
     7921 atacccaaaa gcaagacaga ataaggcaac cttgaataat gttgacatgt tcatctcctg
     7981 aatttcattt tgaaaatcgt taatatcgca agaagaccat ccttgaattt tattttcttt
     8041 ccctgcgcgt atggcctggg ataatatgag ataccaacct gtgttattct aaacttatgc
     8101 tttgctgcaa gaatggtaaa ctcaggctca aactcaaatc catctgaaac aatgttcatc
     8161 tggttgagaa ctttttttga aaatacttta tatccggttt ccatatcaga aatttttgtt
     8221 ccataaagta tgttgcaaag acc
//
LOCUS       DAORYU010000037         8111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_6386_length_8111_cov_6.927135, whole genome shotgun sequence.
ACCESSION   DAORYU010000037 DAORYU010000000
VERSION     DAORYU010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8111
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6386_length_8111_cov_6.927135"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3088)
                     /locus_tag="PLW07_02075"
     CDS             complement(<1..3088)
                     /locus_tag="PLW07_02075"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOL49342.1"
                     /translation="MEKKTEKMLNEFKSPGSIYRGAPFWAWNGKLEPEELRRQIRIMY
                     QMGLGGFFMHSRVGLDTSYLSNEWFECINACTDEAEKLKMLAYLYDEDRWPSGAAGGL
                     VTKNPRYRMRHLCMKIFENIKLVKWSNNVIAAFAVNFEGRKIKDYKKIKKNEKLSRNT
                     TIIVFSVEVQKNSDWYNGYAYLDTMNEQAVKEFIKITHQRYKKYSGKYFGKVIPGIFT
                     DEPNYGSHYFHYIWPAQPEANIVISWTEKLPEVFKKRYGYDILNHLPEIYYDVENQKI
                     SKVRYHYFDCITHLFVNAFAKQIYDWCEKNNILFTGHVLSEETLISQTNVVGSALRFY
                     EYMQAPGMDLLTEHRREFNTAKQVSSVANQFGRKWRLTETYGCTGWDFPFSGHKALGD
                     WQTALGINLRCQHLSWYTMLGEAKRDYPASIFYQSPWWQIYSKVENYFARIYAMLTKG
                     KEIRDLLVIHPIESMWAIFRMPELDSEKKLTERDKDIIEYDRMFQQLCDVFLSNHIDF
                     DYGDEEILSRWAKATKEDGSPVFKVNQARYKVIVVPPLYTIRSSTLNLLKKFNQAGGT
                     VLFVEKMPEYVDGLSTEGNIFDGFEKVNSFEEAVKKLEGKAKRVSITDENGKEIKEIL
                     YLLKEDKDSYYLFLCNTSMEWKNLADRHTLSRDRVIEFNNVTVSGIPDWQYPAIELDP
                     DSGKCYAVETIKKENGYAIKTSFPAIGSRLFIVPKKQLHLKVETRENLKEIQRIKIEK
                     DTWDILLSEPNVFVLDKATYKIGETAWQEKKEILKLDRELRKQLGIPLRGGQMVQPWA
                     REKNKKLKTTPVTLKYVFSIEKIPSGRMCLAIEKPSLYNIDINGYSLSADMKSGWWVD
                     KSLELLEFDPCILKKGENEILIETGYNAEHPGFEIIYLLGNFGVKMVDGTPVITEKVE
                     KLSIGDWTQQGLLFYSGNVTYLHDIHVNIEQNQKIFVNIPEYYGAAVRILVDGKQAGI
                     IAWPPNTIDITEFVADGQDHTLGIEILGHRKNSHGPLHFYQKYPLWTGPYQFIAEGN"
     gene            3378..4382
                     /gene="gap"
                     /locus_tag="PLW07_02080"
     CDS             3378..4382
                     /gene="gap"
                     /locus_tag="PLW07_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOL49343.1"
                     /translation="MRVAINGFGRIGRLVLRAAIEKGMEGFEIVAFNDIADVKTNAHL
                     FKYDSNYGIFQGSVEAKEKEIVINGKPYKVLVEKDPRNLPWKDLKVDMVVESTGLFTE
                     AEKAKAHIEAGAKKVIITAPAKGEDVMIVIGVNDSMYDPAKHHIISNASCTTNCLAPM
                     VKVLDDSFGVVRGLMTTIHAYTNDQRILDLPHKDLRRARAAALSMIPTTTGAAKAIGK
                     VIPKLNGKLNGIAIRVPTSTVSIVDFVAQLEKKVDDKAINEAFKKASETSLKGILQYC
                     DEPLVSKDFVRNEHSCIFDALSTYLIEGDMAKVFGWYDNEWGYSCRIVDLLKLVAKKG
                     "
     gene            4401..5585
                     /locus_tag="PLW07_02085"
     CDS             4401..5585
                     /locus_tag="PLW07_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOL49344.1"
                     /translation="MELITVKDKDYSGKRVFLRVDFNVPLDKNLNITDDTRIRASLDT
                     INYLLSQNAVVICASHLGRPKGKPVAEMSLDPVAKRLSELTGKQVKFAKDCVGAEAKK
                     VVDSAKSGDVVLLENLRFHPEEEENDESFSKSLAELADCYVNDAFGSSHRAHASIVGI
                     TKFLQDKASGFLIEKEVNALSKLLEKPERPFLLIIGGAKVADKIGMIKNLLDKVDTII
                     TGGVMAYTFIRANNWETGNSKVEADAIPLAKEIYKELRKRHLEFHTPLDHVVADKIAE
                     DAHYFTTERGTVPIGWTGVDIGPQAVEEYADCIRRSKTIFWNGPMGVFEIEPFAKGTI
                     EIAKAVAASNAFSVVGGGDSIAAINKAGVADKISHICTGGGASLEFLEGKGLPGIDIL
                     KK"
     gene            5593..6345
                     /gene="tpiA"
                     /locus_tag="PLW07_02090"
     CDS             5593..6345
                     /gene="tpiA"
                     /locus_tag="PLW07_02090"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOL49345.1"
                     /translation="MIKPLIAGNWKMYKTPSEAELFALELKKISKQNDRDVLVCVPFP
                     SLMVVSKVLKDSGIFVGAQNVYPAKEGAFTGEVSPFMIKDAGASFVICGHSERRQIFK
                     ETDQFISEKVKSVVECGMTPILCVGETLQQNEKGETFSVVESQLKTGLQHISSALINN
                     VVIAYEPVWAIGTGKNATPQQAQQVHSFIKSVLEKIYGGSSKMLRVLYGGSVKPENID
                     ELMTQPDINGVLVGGASLKIESFSRIISFKKN"
     gene            6361..6708
                     /gene="secG"
                     /locus_tag="PLW07_02095"
     CDS             6361..6708
                     /gene="secG"
                     /locus_tag="PLW07_02095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOL49346.1"
                     /translation="MMTFLIVVHLIVSIMLIVAVLLQSGKGSSMANVFGGGGMETVFG
                     AETPAIMNRITTILAVLFMVNCILLTTVSFKYRGGSVIQREVQKGSVPQSEEPVQSVP
                     TTPSTNVPPAQNN"
     gene            6719..>8111
                     /locus_tag="PLW07_02100"
     CDS             6719..>8111
                     /locus_tag="PLW07_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOL49347.1"
                     /translation="MFFFVFACTVRAIEIKPGGSIVISASSIPKSFNPVVAKETSTTF
                     VTGFIFEGLIATDGVTLKIKPNLAESWDIDATGRIWTFHLRKDVYWNDGVQFTAKDVE
                     FTFNRLIFNPEIPTSSRDIFTINNQSIKVKAIDNFTVRFILPDRFAPFLRLMGQEILP
                     EHKYRKDVDENKFSSAMGLNSKPQDIVGTGPFMLEDFRPGEWVILKKNKMYWKYDSNG
                     NQQPYIDRIIILTIPDQNMALLKFKKGELDMISLRAQDYLLLKRANLPGVKFYNVGPS
                     LSSQFIIFNQNLDSNIPEYKKEWFRNINFRRAVSCAIDRQTIINNIFAGLGYPLYGPV
                     NESVQEFYNPRIKKFPYNLDEARKYLVEGGFKAGKDGLLYDSKGNQVSFSLITNSNSS
                     ERIEQANLIRNDLEKLGMKVNFLPVDFNSLVTRINATKDWESVIIGLTGGIDPHFGKN
                     VWVTTGHLHLWNLG"
BASE COUNT         2442 a         1428 c         1645 g         2596 t
ORIGIN      
        1 catttccttc tgcaataaac tgatatgggc ctgtccacaa aggatatttc tgatagaaat
       61 gcaaaggacc atgagaattt tttctgtgtc ccagaatttc tatcccgaga gtatgatctt
      121 gcccatctgc gacaaattct gtaatatcaa tggtgtttgg tggccaggcg atgattcctg
      181 cctgttttcc atcaacaaga atccgtactg ctgcgccata gtattcagga atgtttacaa
      241 aaattttttg attttgctca atgttcacat gaatgtcgtg aagatatgtt acattgcctg
      301 aataaaagag taatccctgc tgtgtccaat ctccaataga aagtttctcc actttttctg
      361 taattaccgg ggtaccatca accattttta ctccaaaatt tccaagtaaa taaataatct
      421 caaatccagg atgttctgcg ttatacccgg tttcaattaa tatttcgttt tctccttttt
      481 taaggataca tggatcaaat tctaataatt caagagattt atcaacccac caccctgatt
      541 tcatgtcagc agataatgaa taaccgttaa tatcaatgtt gtaaagtgat ggtttttcaa
      601 tggcgaggca cattctacct gatggaattt tttcaattga aaatacatac tttagtgtaa
      661 caggagtagt tttaagtttt ttattttttt ctcttgccca cggttgaacc atttgtccgc
      721 ctcgcagagg aataccaagt tgttttctca attctctgtc gagcttaaga atttcctttt
      781 tctcctgcca ggcggtttct ccaatcttat acgttgcttt atcaagcaca aacacatttg
      841 gttctgacag taagatgtcc caggtatctt tttcaatttt tattctttga atttctttca
      901 ggttttctct tgtttcaact tttaaatgaa gttgtttttt cggtactata aagagtctgc
      961 ttccaatggc aggaaaagat gtttttattg catacccatt ttcctttttt attgtttcta
     1021 ctgcatagca tttccctgaa tcagggtcaa gttcaatggc tggatactgc cagtcaggta
     1081 ttcctgatac tgtgacattg ttgaattcaa tgactctatc tcttgaaaga gtgtgtctat
     1141 cagcaagatt tttccattcc atagaagtat tacacaaaaa cagataatat gaatccttgt
     1201 cttcctttaa tagataaaga atttccttta tttcttttcc attttcatca gtaatggata
     1261 ccctttttgc cttgccttca agttttttta ctgcctcttc gaaagaattt actttttcaa
     1321 aaccgtcaaa aatatttcct tcagtggata atccgtcgac atattcaggc attttttcca
     1381 caaacagtac agttccacct gcttggttga attttttcaa caaattcaat gtagaacttc
     1441 tgattgtata aagaggcgga acaacaatta ctttatatct ggcttgatta actttaaata
     1501 ccggagaacc atcttcttta gttgcttttg cccatcgaga gagaatttct tcgtcgccgt
     1561 aatcaaaatc aatgtggttt gagagaaaca catcacacag ttgctggaac attctgtcat
     1621 attcaataat gtctttatct ctttcagtca attttttttc actgtcaagt tctggcattc
     1681 tgaagattgc ccacatgctt tcaatgggat gaataacaag aaggtctctt atttcttttc
     1741 cctttgtcaa catagcatag attctggcaa aatagttttc taccttgcta taaatttgcc
     1801 accagggaga ttgataaaaa atacttgcag gataatctcg ttttgcctca ccaagcattg
     1861 tataccatga taagtgctgg catcttaagt tgattccaag cgcagtctgc cagtcaccaa
     1921 gggctttatg tcctgaaaat gggaagtccc agccagtgca tccatatgtt tctgttaatc
     1981 tccattttct tccaaactgg tttgccacag aactgacctg ttttgcagtg ttgaattccc
     2041 ttctatgctc ggtaagaagg tccataccag gcgcctgcat atactcataa aatctaaggg
     2101 cacttccaac aacattcgtc tgtgagatga gcgtttcttc agacaaaaca tgtccagtaa
     2161 aaagaatgtt gtttttttca caccagtcgt aaatctgttt tgcaaaggca ttgacaaaaa
     2221 ggtgcgtaat acaatcaaaa taatggtatc tcacttttga tattttttga ttttcaacat
     2281 cgtaatagat ttcaggcaaa tggttgagaa tatcataacc atatcgtttt ttaaaaacct
     2341 ctggcaattt ttctgtccag ctgataacaa tatttgcttc tggttgggct ggccagatgt
     2401 agtgaaagta atggcttcca taatttggtt catcagtaaa aattccagga atgacttttc
     2461 caaaatattt cccggaatat tttttatacc gctgatgagt aatttttatg aattctttta
     2521 ctgcttgttc attcattgta tcaagataag catagccatt gtaccagtca gaattttttt
     2581 gtacttcaac agaaaagaca ataattgtcg tatttcggga taacttttca tttttcttga
     2641 tttttttata atcttttatc tttcgtcctt caaaatttac cgcaaatgca gcaatgacat
     2701 tgttagacca tttgaccagt tttatgtttt caaatatttt catgcaaaga tgtcgcattc
     2761 tgtacctggg attttttgtg acaaggcctc ctgcagcacc tgatggccat ctatcttcat
     2821 cataaagata ggcaagcatc ttaagttttt ctgcttcatc agtacaggca ttgatgcatt
     2881 caaaccattc gtttgaaaga tatgatgtgt caagacccac gcgggaatgc ataaaaaatc
     2941 caccaagtcc catttgatac ataattctta tctgacgtct caattcttct ggctcaagtt
     3001 ttccattcca tgcccagaaa ggagcgcctc tatagatact acctggtgat ttgaattcgt
     3061 ttaacatttt ttctgttttc ttttccataa tatttccttt gtaattttat agttcaggtt
     3121 tgccattgaa taagaatata ataaatttgt tttcagtcaa gttaatctat atgggtcgaa
     3181 gataccgagc ttagcgcaat ggcagttttg ccagacatct taccctgcaa aatcaagacc
     3241 acatactttt gtgaaaacat tggagtttaa ctgcagcaat ttatgaacga actcgtgggt
     3301 aagtcagatt ttgacactat ccagtaaaag gtgtagaata atattaccat cctaacaaat
     3361 acctgaaagg aggaattatg agagttgcga ttaacggttt tggaagaatt ggaagactgg
     3421 tattaagggc agcaattgaa aaaggaatgg agggttttga aatagtagcg ttcaatgata
     3481 ttgcggacgt aaaaacgaat gctcaccttt ttaagtatga ttcaaactac ggtatttttc
     3541 agggtagtgt tgaagcaaag gaaaaagaaa ttgtaattaa cgggaaacca tacaaggttc
     3601 ttgttgagaa agatccgaga aatcttccct ggaaagattt gaaggtggac atggttgttg
     3661 aatcaaccgg gcttttcaca gaagcagaga aagcaaaggc acatatagaa gcaggtgcaa
     3721 aaaaggttat tatcactgct cctgccaagg gtgaagatgt gatgattgtt attggagtga
     3781 atgactcaat gtatgacccc gcaaaacatc atattatttc aaatgcatcc tgtactacaa
     3841 actgtcttgc tccaatggta aaggttcttg atgactcatt cggcgttgta aggggtttaa
     3901 tgacaaccat tcatgcctac acaaatgacc agagaattct tgaccttccc cacaaagacc
     3961 ttcgaagggc aagagcagcg gctttgtcaa tgattccgac cacaacaggc gctgcaaaag
     4021 caattggtaa ggttattcca aaattaaacg ggaaattaaa tggtattgca ataagggttc
     4081 cgacatccac agtgtcaata gttgattttg tcgcccaact tgagaaaaaa gttgacgata
     4141 aagcaatcaa cgaggcattc aaaaaagcgt cagaaacgtc gttgaagggt attctgcaat
     4201 attgtgatga accacttgtt tcaaaggatt ttgtcaggaa tgaacattcc tgtatttttg
     4261 atgccctatc gacctatctc attgaaggag atatggcgaa agtgtttgga tggtatgaca
     4321 atgagtgggg atatagttgc aggattgttg accttttgaa actcgtagca aaaaaaggtt
     4381 aagtttcccg ggaggaaaca atggaattga taacagttaa ggacaaagat tattcaggaa
     4441 agcgtgtttt tcttcgggtt gactttaatg tccctcttga caaaaattta aacatcacag
     4501 atgatacgag aatcagagcg tcactggata caattaatta tttgttgagt cagaatgcgg
     4561 tcgtgatttg tgcttcacat cttggaagac caaaaggaaa accagttgca gaaatgagcc
     4621 ttgaccctgt ggcaaagcgt ctttctgaac ttaccggcaa acaggtgaaa tttgctaagg
     4681 attgtgtggg agcagaagca aaaaaagttg ttgattccgc aaaatcagga gatgttgttc
     4741 tacttgaaaa tttgagattt catcctgaag aagaggaaaa tgatgagtcc ttttcaaaaa
     4801 gtttggcaga acttgcggat tgttatgtaa acgatgcctt tggttcatcc catcgtgctc
     4861 atgcttcaat tgttggtatc acaaaattcc ttcaggataa ggcatctggc tttctgattg
     4921 aaaaagaggt gaacgcgttg agtaaactgc ttgaaaaacc agaaagacct tttttgttga
     4981 ttataggcgg agcaaaggtt gctgataaaa tagggatgat aaaaaatttg cttgataagg
     5041 tggatacgat tattactggc ggtgttatgg cttatacctt tatcagggcg aataattggg
     5101 aaacaggaaa ctcaaaggtt gaagcagacg caattcctct ggcaaaagag atttataagg
     5161 aattgagaaa acggcatctt gaatttcata ctccgctgga tcacgttgtt gctgataaaa
     5221 tcgcagaaga cgctcactat tttacgaccg aaaggggtac tgttcctatt ggatggactg
     5281 gcgtggacat tggaccacag gcggttgaag agtatgctga ttgtatcaga aggtcaaaaa
     5341 caatattctg gaacggcccg atgggtgttt ttgaaataga accgtttgca aaagggacta
     5401 ttgagattgc aaaagcagtt gcagcaagta atgcgttttc tgtagttggc ggcggtgatt
     5461 caatagcagc aatcaataag gcaggcgttg cagataaaat ttctcacatc tgtacaggtg
     5521 gcggtgcttc tcttgaattt cttgaaggaa agggattgcc tggaatagat attcttaaaa
     5581 agtgaggaaa aattgataaa accacttatt gcgggcaact ggaaaatgta taaaacgccc
     5641 tcagaagcag aattatttgc tttggagttg aaaaaaattt caaaacaaaa tgacagggat
     5701 gttcttgttt gtgtaccgtt tccttcatta atggtagtca gtaaagtact taaagattcc
     5761 gggatttttg ttggagcgca aaatgtgtat cctgcgaaag aaggtgcttt tacaggtgag
     5821 gtcagtccgt tcatgattaa ggatgctgga gcatcttttg ttatatgcgg ccactcagaa
     5881 agaaggcaga tttttaaaga gacagaccag tttatatcag aaaaggtaaa aagtgttgtt
     5941 gagtgtggga tgacgccgat tttgtgcgtt ggtgaaacac tccagcagaa tgaaaaaggt
     6001 gaaacttttt ctgtggttga aagtcagttg aaaaccggtt tacagcacat ctccagcgct
     6061 cttatcaaca atgttgtcat agcgtatgaa cccgtctggg caataggtac tgggaaaaat
     6121 gcaacccccc agcaggctca acaggttcac tcctttataa aaagtgtgct tgagaaaatt
     6181 tatgggggat cttctaaaat gcttcgggtt ctttatggcg gtagtgtgaa acccgaaaac
     6241 atagacgaat tgatgactca gccagatatt aacggtgtcc ttgttggtgg agcaagtttg
     6301 aaaatagaat ctttttcgag aataatatct ttcaagaaga attgaaattg cgaggttcat
     6361 atgatgacat ttttaatcgt tgtccatttg attgtatcaa tcatgttgat agtggcggtg
     6421 cttcttcaga gtggcaaggg ttcttctatg gcaaatgttt ttggcggggg aggaatggag
     6481 accgtatttg gtgcagagac acccgcgatt atgaacagaa ttacaacaat acttgctgtt
     6541 ttgttcatgg ttaattgtat tcttcttacc actgtttcat ttaagtacag aggtggttct
     6601 gtgattcagc gtgaagtaca gaagggttct gttcctcaat cagaagaacc agtgcaatcg
     6661 gttccaacaa caccttccac aaatgtgccg ccggcacaga ataattgatt tttgtttttt
     6721 gtttttcttt gttttcgcgt gcactgtcag ggcgatagaa ataaaacctg gcgggtcaat
     6781 tgtaatttca gcgtcatcaa taccaaaatc atttaatcca gtcgttgcaa aagaaaccag
     6841 tactactttt gttacagggt ttatttttga aggtttgatt gcaactgatg gtgttacctt
     6901 aaaaatcaag ccaaatcttg cggaatcctg ggatatagat gcaaccggta gaatatggac
     6961 atttcattta agaaaagatg tttactggaa tgatggtgtc caatttaccg caaaggatgt
     7021 tgagtttaca tttaaccgtt tgattttcaa tcctgaaatc cctactagca gcagggatat
     7081 ttttacaata aacaatcagt caatcaaggt gaaggcgatt gataacttta cagtgaggtt
     7141 tattttgcca gacaggtttg caccatttct caggttaatg ggtcaggaga tacttcctga
     7201 acataaatac aggaaagatg ttgatgaaaa caaattttca tctgccatgg gtctcaattc
     7261 aaaaccgcag gatattgtgg gaacaggtcc tttcatgctt gaagattttc gtcctggaga
     7321 atgggtaata ttgaaaaaga acaagatgta ctggaaatac gattcaaacg gcaaccaaca
     7381 gccctatatt gatagaatta ttattcttac aattcctgac cagaatatgg ctctgttgaa
     7441 gtttaaaaag ggcgaacttg acatgatttc gttaagagca caggattatt tgttacttaa
     7501 acgggcaaat cttcccgggg tgaaatttta caatgtcgga ccatcattga gttctcaatt
     7561 tattattttt aatcaaaatc ttgatagcaa tattcctgaa tacaaaaaag aatggttcag
     7621 aaatataaat tttagaaggg ctgtttcatg tgcgattgac aggcaaacaa ttatcaacaa
     7681 tatttttgct ggtctgggat atccgctcta cggcccggta aatgaatctg tccaggaatt
     7741 ttacaatccc agaataaaaa aatttcctta taatcttgat gaagcaagga aatatcttgt
     7801 tgaaggtgga tttaaagcag gtaaagatgg ccttctttat gattctaaag gtaatcaggt
     7861 ttctttttct ctcattacaa acagtaatag ttctgagagg attgagcagg caaacctgat
     7921 aaggaacgat ctcgaaaaac ttggtatgaa ggtgaatttt cttcctgttg atttcaattc
     7981 tcttgtgacc aggattaatg caacaaaaga ttgggaatct gttattattg gactgactgg
     8041 tggcattgac cctcactttg gaaagaatgt ttgggttaca accgggcatc ttcatttatg
     8101 gaatcttggt t
//
LOCUS       DAORYU010000038         8055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_6440_length_8055_cov_4.691500, whole genome shotgun sequence.
ACCESSION   DAORYU010000038 DAORYU010000000
VERSION     DAORYU010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8055
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6440_length_8055_cov_4.691500"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="PLW07_02105"
     CDS             complement(<1..1136)
                     /locus_tag="PLW07_02105"
                     /inference="COORDINATES: protein motif:HMM:NF020120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase N-terminal
                     domain-containing protein"
                     /protein_id="HOL49348.1"
                     /translation="MFESAKWIWIDENPVDAFQICYFRKKFIVGKKGSYLIHCFADSK
                     YRLWINGKYIGFGPARGKAYRPYYDTYRINLKEGENVIAFSVQYYCQPTKIFESIEPG
                     LICQIESEGNTILVSDSSWKACVAKSYSCVPGIFFPECFDARLEQKNWQQLSFDDSEW
                     KNAIEKEFSDLPKPENFLPRPIPLIKEKVVVPQRILDIYECPTENIQDFLDKEKIAET
                     LWSAKKLETKVSVEPEIKIPCRWKKNLAFTLEGQQAICVIVDFGFETHAFIEICAEGP
                     SGTMIDFAHSECLWNNKVATLWQSPSLKQAQRIILGNGKVCHRTNQQRGFRYMVVRIF
                     NPADTKINVTLHSIVGYEAIYPVKKQGCFQCSDKLFEQIYNLSA"
     gene            complement(1151..2005)
                     /locus_tag="PLW07_02110"
     CDS             complement(1151..2005)
                     /locus_tag="PLW07_02110"
                     /inference="COORDINATES: protein motif:HMM:NF013425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOL49349.1"
                     /translation="MENIFLPERKKDSHKGTYGHLFVIGGSPGLTGAVCLSCLAALRT
                     GCGMVTAGIPESLNDIFEIKLTEVMTKPLPESGRRTISMLAVEKCVDFATKVADGIVI
                     GPGISTDFATEEFFKNFLPQINKPIVVDADGLKLLAKNLKILDKQKKIILTPHPGEMS
                     HLTKMPISEIQKNREKVAKDFAKKYGVVVVLKGYRTVISDGERIYINLTGNPGMATAG
                     SGDVLSGIIGALVVQGFSLWDSAVIGTFLHGLAGDLAARKIGEYSLIAGDIIDFLPEA
                     IRIIQKKS"
     gene            complement(1962..2336)
                     /locus_tag="PLW07_02115"
     CDS             complement(1962..2336)
                     /locus_tag="PLW07_02115"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOL49350.1"
                     /translation="MMSSGIETIDVKKIAVLLKSAEYKKYFTPSEVDYCRRYKDMYIH
                     LAGCLAAKFAFLKAISPFSNFDFSDVEIRHANSGKPFVSVSKNHNFTISLSISHTKTV
                     AVALCVITDGKHFSARKKKRFS"
     gene            complement(2333..2581)
                     /locus_tag="PLW07_02120"
     CDS             complement(2333..2581)
                     /locus_tag="PLW07_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOL49351.1"
                     /translation="MGRKKQGNGRDSNPKMRGIKVFEGQIVKAGNIIVRQKGTHFVPG
                     PGTYIGSDCTIHAAREGMVVYGTSRGKKLVSVISPSTS"
     gene            3275..4408
                     /locus_tag="PLW07_02125"
     CDS             3275..4408
                     /locus_tag="PLW07_02125"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOL49352.1"
                     /translation="MNSRERFLRCHLFKEVDHVPFVEIAVWTETIKRWLKEGLPADVD
                     ATFYINGMIGNEFFGFERWEYIPLRTDMIPRFEYQVIEEDERTITARHGNGIVTKALK
                     VGTVRGTRMSMDQYLSFPVTDRKSFYEMKKRYNPYSPARYPRWWNDLIACYKNRSYPL
                     ALTGIGGFGLYSMLRNWMGTENACTIFYDDPSFAEEMLDFLVEFFVQLTEKALNDVDV
                     DWYNYFEDFAYKTGPLVSPCIFKKFLLPRYKQINGYLIKHGVKIISLDSDGNIEVLLP
                     LIIEAGFNHICPMEQAAGMDAVKIRKTYGSDLAMLGSIDKRELAKGKKEIERELLRQV
                     PYLLETGGYIPTIDHAVPPDVSYGNFMYYLEFKKKLLAGKYGI"
     gene            4421..5506
                     /locus_tag="PLW07_02130"
     CDS             4421..5506
                     /locus_tag="PLW07_02130"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOL49353.1"
                     /translation="MTKRERYLKALRNQQVDCLVWAPNFDYWLHVNTNEGTLPEKYKG
                     MSRNDIVRAIGGYIWNRQQGIKTVYDRSVKIKYDTVNDCSITEISTPVGIVRCVHKKT
                     EGESRSKHLSEHFIKNVDDIKVMKYIAEAMRFEPDYEPTLKALEETGQDGVVLNGCFS
                     VPFIQFAKIDAGYINAFYLWVDYRKEVDELLNVYLKKYLEAIKILADGYADVIATGDN
                     MDGVMITPQIFKEYAIPYYQECKKILSEKGKIFEGHWCGRTENLLPLVPGCGLDVVEA
                     IVTAPMAKITLKEALDMLDNKVVLQGGIPSVLVCKEGGTREDFIRYINEVIVPLKGRR
                     SFILGMSDNVPPNADFERVEMVSKLIS"
     gene            complement(5501..5947)
                     /locus_tag="PLW07_02135"
     CDS             complement(5501..5947)
                     /locus_tag="PLW07_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013514784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOL49354.1"
                     /translation="MKYSQAEIKRVFVVRLEDGDKLPDVIEDFARKHNIQCGVCILIG
                     GIGSGKLVTGPENPGDSPIIPIFQNITGTSEIAGIGTIFPDENNIPRLHMHATTGRRK
                     TTLTGCIRPGITVWKIGELIILELTGTKAKRVKDKRAGFSVLELDL"
     gene            complement(5944..7047)
                     /locus_tag="PLW07_02140"
     CDS             complement(5944..7047)
                     /locus_tag="PLW07_02140"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOL49355.1"
                     /translation="MQYRKFGNTSYNVSILGFGAMRLPMLPDGTVDFEKAAEIIRYAI
                     EHGVNFIDSHHFYHNGQSEEAIGRAIKNFPREKLIIQTKIGMYNNYTDDQCWQLLEAA
                     LKKLNTDYIDFYLTHSLNWETYTRHNKQFMKFTQKALDQKLIRYRGFSVHDSLEKMKK
                     IINTGEFSAMTVQYNLLYRESEEAIALAHEKGIGVVVMGPVGGGTFGYPGETLLSWIE
                     KNEKSTAALALRFVLSNPCVDVAISGMSNLQQVMENIETVSDFSPLTQDEIKKIDEIF
                     ESRKKLLNLYCTGCRYCMPCPNNVNIPSNFHWYNVANVYGVEKRAHNGYFALKVEERA
                     SACTECGKCEEKCPQKIPIREKLKEVAKYFEKK"
     gene            complement(7086..>8055)
                     /locus_tag="PLW07_02145"
     CDS             complement(7086..>8055)
                     /locus_tag="PLW07_02145"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOL49356.1"
                     /translation="KGLCGMQPVSRMDDIVLLRPMLSISKKQILSYLEKKKIRYMIDE
                     SNLDTKFARNFLRHQVLPLLEKINPAYKKNISRTAKILGDEYAFLESFTEKKIKKYAV
                     EKNGCIEFSRGMFVRQQVSIQRFFLRQILIRMSGSTYPPDFATVENLRILILQKKQCF
                     LNKLKIYATCNGNTVSVCKKIDEKEIILKKPVQIIIPGETIVPETGWKIVTEYTDYSE
                     EFLNNQDILVAYVNAEKVEKTLMLKQPDRNERFVPLGMGKQVSFKKYWKTHKKTLKDI
                     TKIPLAIEEGKNRGKIIWIIGGHISQKFGIEKNKPVLKISCKRIYV"
BASE COUNT         2425 a         1529 c         1521 g         2580 t
ORIGIN      
        1 ctggctgaaa gattgtaaat ctgctcaaaa agtttatcag aacactggaa acaaccctgc
       61 ttttttacag gatatattgc ttcatagccg acgattgaat gaagtgtgac atttatcttt
      121 gtatcagcag gattgaaaat tcttacaacc atatatctga atccacgctg ctgatttgta
      181 cgatgacaca cctttccatt tccgaggata attctttgcg cctgcttcaa cgaaggtgat
      241 tgccatagcg ttgcaacttt gttattccag aggcactcgc tgtgagcaaa atcaatcatt
      301 gttcctgaag gaccttcagc acatatttca ataaatgcat gcgtctcaaa tccaaaatcc
      361 acaataacac atattgcctg ttgtccttca agcgtaaaag caagattttt cttccagcga
      421 catggaattt ttatttctgg ctcaacagaa acttttgttt caagtttttt tgcactccag
      481 agtgtctcgg caattttttc tttatcaaga aaatcttgaa tgttttcagt gggacattca
      541 taaatatcaa gtattcgctg tggcacaacg accttttctt tgatcaatgg aatgggtctt
      601 ggaagaaaat tttcgggttt cggtagatca gaaaattctt tttcaatagc atttttccat
      661 tcagaatcat caaaggacag ttgctgccag tttttctgtt caagccgtgc gtcaaaacat
      721 tcaggaaaaa aaatgccagg cacacaacta taggattttg caacacacgc cttccatgaa
      781 gaatcagaca caagtattgt gtttccttct gattcaatct gacagataag tcctggttca
      841 atggattcaa aaatctttgt gggctggcaa taatactgaa cagaaaatgc aatcacattt
      901 tcaccctctt tgaggtttat cctgtaagta tcataatatg gacggtacgc ctttcccctt
      961 gcaggaccaa aaccaatgta ttttccatta atccagagtc tgtattttga atcagcaaag
     1021 cagtgaatca ggtagcttcc cttttttccc actataaatt tttttctaaa ataacatatc
     1081 tgaaatgcat caactgggtt ttcatcaatc catatccatt ttgcagattc aaacattttt
     1141 attcctcctc ttatgatttt ttttgtatta ttctaatcgc ctctggcaga aaatcaatga
     1201 tgtctcctgc aataagcgaa tactcgccaa tttttcgtgc cgcgaggtct cctgcaagac
     1261 cgtgaagaaa tgttccgatg acagcactat cccacaaaga aaatccctga acaacaagtg
     1321 cgccaataat accagaaaga acatctccgc ttcccgcagt tgccatacca ggatttcctg
     1381 tcagattgat gtatattctt tctccatcag aaatgactgt tctgtatcct ttaaggacaa
     1441 caacgacacc atattttttt gcaaagtcct ttgcaacctt ttctctattt ttttgaattt
     1501 cactgattgg catttttgta agatgagaca tttcgcctgg atggggagtg agaataattt
     1561 ttttctgttt atcaaggatt tttaaatttt ttgcaagaag tttcaaacca tcagcgtcaa
     1621 cgacaatcgg tttattaatc tgtggcagga aatttttaaa aaactcttct gtggcaaaat
     1681 ctgtggaaat tccaggtcct atcacaatac catctgcaac tttggtagca aaatcaacac
     1741 atttttcaac tgcaagcata ctgattgttc ttcttccaga ttcaggcagg ggttttgtca
     1801 ttacctctgt aagttttatt tcaaagatgt cattgaggga ttctggtatt ccagcagtta
     1861 ccattccaca acctgttctt aaagcagcaa gacaactgag acacactgcg cctgttagcc
     1921 cgggcgaacc accaataaca aacagatgtc cgtaagttcc cttatgagaa tctttttttc
     1981 tttctggcag aaaaatgttt tccatctgtt attacgcaga gtgccactgc aactgttttt
     2041 gtatgggata ttgatagtga aattgtaaaa ttgtgatttt tagatacact gacaaatggt
     2101 ttgccagaat ttgcatgcct gatctcaaca tcagagaaat caaaattcga aaaaggggaa
     2161 attgctttca gaaaagcaaa ttttgcagca agacagccag caaggtgtat atacatgtct
     2221 ttatacctgc ggcagtagtc aacctcagaa ggggtaaaat attttttata ttctgcgctt
     2281 tttaaaagaa ctgctatttt ttttacatca atggtttcaa taccagacga catcatgaag
     2341 tagatggact gatgacactg acaagttttt tacccctgct tgtgccataa acaaccatgc
     2401 cttctcttgc agcatgaatt gtacagtcag agccgatata tgtccctgga ccaggaacaa
     2461 aatgagtccc cttctggcgg acaatgatat tgcctgcctt cacaatctgg ccctcaaata
     2521 ctttgatacc ccgcattttg ggatttgaat cccttccatt accctgtttt tttcttccca
     2581 tagtccaata atcttaacat aattttttat acaagtcaac ggaatttttt cataggttga
     2641 aaaaattgtt atactgcctc actaagggga gatagaaaat aaggtagaat agtgtgttct
     2701 gaaatacggg gcagtacaac aaaatcacac ttttctttgc aaaatcaact catactgcct
     2761 cccatcttta taaaatcatt tcttattatt tcccttcttt gtaaaattat ctcatatacc
     2821 attcccatct ttataaaatc acctcatact acccccctct ttataaaaga ggggttgggg
     2881 gagatttcaa aaaatccccc ttagtccccc ttttgaaaag gggagacctg gatgaggaat
     2941 ttgtaaaggg ggagaatgag ggtggtagag gggctggggg agatttcaaa aaatccccct
     3001 taatccccct tttgaaaagg gggatatcag gagagaaatt ttgtaaaggg ggttaacgag
     3061 aaggtaaagg aagtctggga ttgatttgat gaaatcccct taatccccct tttgaaaagg
     3121 ggagacctgg atgaggaatt tgtaaagggg gatatcagga gagagattta gtaaaggggg
     3181 atatcaggag agaaattttg acaaatgggg ggcagcacaa tatgatatat catgactgcg
     3241 tcacaaacat cagtacacaa agccaaaaga aaaaatgaat tccagagaaa gatttctcag
     3301 atgtcatctg ttcaaagaag ttgaccacgt tccatttgtt gaaattgctg tatggactga
     3361 aacaatcaaa agatggttga aggaagggct tcctgcagat gttgacgcaa cattttacat
     3421 aaatggcatg attggcaatg aatttttcgg atttgaaaga tgggagtata ttccattacg
     3481 cacagacatg attcctcgtt ttgaatatca agtgattgaa gaagacgaaa ggaccatcac
     3541 agcaagacat ggaaatggta tcgtgacaaa ggcattaaag gtgggaactg tccgtggaac
     3601 aagaatgagt atggaccaat atttaagttt tcctgttacc gacagaaaaa gtttttatga
     3661 aatgaaaaaa agatacaacc cctattcccc cgccagatat ccacgatggt ggaatgacct
     3721 tattgcctgt tataagaaca ggagttatcc tctggctctt acaggcatcg gtggttttgg
     3781 tctctacagc atgctaagaa attggatggg tacagaaaat gcatgtacta tattttacga
     3841 cgacccatct tttgctgaag agatgcttga ttttctggtt gagtttttcg ttcaactgac
     3901 agaaaaagca ttaaacgatg tcgatgttga ctggtacaac tattttgagg atttcgcata
     3961 caaaacagga ccacttgttt ctccatgcat ctttaaaaaa tttttattgc caagatacaa
     4021 acaaattaac ggatatctga taaaacatgg agtgaaaata atcagtcttg atagtgatgg
     4081 gaatattgaa gtacttcttc ctcttattat tgaagcaggc tttaatcata tatgccctat
     4141 ggaacaggca gctggcatgg atgccgtgaa aatcagaaaa acttatggca gtgaccttgc
     4201 aatgctggga agtattgata agagggaact cgcaaaggga aagaaagaaa ttgaacgaga
     4261 attgttgagg caagtgcctt atttactgga aacagggggg tatatcccta caatcgacca
     4321 tgcagttccc cctgatgtgt cgtatggaaa ttttatgtat taccttgaat ttaagaaaaa
     4381 actcctagca ggaaagtatg gaatttaaag tggaggaaaa atgacaaaga gagaaagata
     4441 tttgaaggca ttgagaaatc aacaggttga ctgtctggta tgggcaccaa attttgacta
     4501 ctggttgcac gtcaacacca atgaagggac actgcctgaa aaatataagg ggatgagcag
     4561 aaatgatatt gttcgcgcaa tcggcggata tatctggaac aggcaacagg gcataaaaac
     4621 agtatatgac agaagcgtaa aaatcaagta tgacactgta aatgattgct caattactga
     4681 aataagtaca cctgttggta ttgtgagatg cgtgcataag aaaacagaag gcgagtctcg
     4741 atcaaagcat ctctctgaac attttataaa aaatgtagat gatattaaag taatgaaata
     4801 cattgcagaa gcaatgaggt ttgagccaga ttatgagcca acattgaaag cgcttgagga
     4861 aacaggtcag gatggagttg tattaaatgg ctgtttttct gtcccattta ttcagtttgc
     4921 caaaattgat gccggttata taaatgcctt ttatttatgg gttgactata gaaaagaggt
     4981 tgacgaatta ctgaacgttt atcttaagaa atatcttgag gcaataaaaa tccttgcaga
     5041 tggttatgca gacgtgattg ctactggtga taatatggat ggagtgatga ttacaccaca
     5101 aatattcaaa gaatatgcaa ttccctacta ccaggaatgc aaaaagatac tcagtgaaaa
     5161 aggaaaaatt tttgaaggtc actggtgtgg aagaactgaa aaccttcttc cacttgttcc
     5221 aggatgtggc cttgatgttg tggaagcaat tgtgactgcg ccgatggcaa agattacatt
     5281 aaaagaagcc cttgatatgc ttgacaacaa ggtcgttctt cagggaggca ttccatcggt
     5341 gcttgtttgc aaggaaggtg gaacaagaga ggatttcatc aggtatatca atgaggtcat
     5401 tgtgcctttg aaaggaagaa ggtcttttat tcttggaatg agcgataatg ttccgccaaa
     5461 tgctgatttt gaaagggttg aaatggtgag caaacttatt tcataaatcc agttcaagaa
     5521 cagaaaatcc tgctcttttg tcctttactc tttttgcctt tgtaccagta agttcaagga
     5581 tgatcaattc acctattttc cacacagtaa ttccaggtct gatacaacct gtcagtgtgg
     5641 tttttcttct gcctgtcgtt gcatgcatat gcaatcttgg aatattattt tcatcaggaa
     5701 aaattgtacc gatgccggct atttcacttg ttccagtgat attttgaaaa ataggtataa
     5761 tcggagaatc tcctggattt tcaggaccag taacaagttt tccacttcca attcctccaa
     5821 tcaaaataca tacaccacat tgaatgttat gtttccttgc gaaatcttca atgacgtcag
     5881 gcaatttatc accatcttca agccgcacaa caaaaaccct ttttatctct gcctggctat
     5941 atttcatttt ttttcaaaat attttgcgac ctctttcaat ttttccctta tgggaatttt
     6001 ctgaggacat ttttcttcgc acttgccgca ttctgtacag gcggatgctc tttcttctac
     6061 cttgagagca aaatacccgt tgtgtgcacg tttttccaca ccgtacacat ttgcaacatt
     6121 ataccagtga aagtttgaag gaatgttaac attgtttgga catggcatgc aatacctgca
     6181 tccagtgcag taaagattga gcagtttttt tcttgactca aaaatttcgt caattttttt
     6241 gatttcatcc tgagttaacg gtgaaaaatc actcactgtc tcaatgtttt ccatcacctg
     6301 ctggagatta ctcatccctg aaatagcaac atcaacacag ggatttgaaa gaacaaacct
     6361 taatgcaagc gctgctgtgc ttttttcatt tttttctatc caggaaagaa gtgtttctcc
     6421 aggatatcca aatgtaccgc caccaacagg acccattaca actactccaa tacctttttc
     6481 atgagcaagc gcaattgctt cttcgctttc tctgtataac agattatatt gaaccgtcat
     6541 tgctgaaaat tcgccggtat tgataatttt tttcattttt tcgagtgagt catggacaga
     6601 aaatccacga taccttatca gtttctgatc aagcgctttc tgggtaaatt tcataaattg
     6661 tttattgtgc ctggtgtatg tttcccaatt taaactgtgt gtcaggtaga aatcaatata
     6721 atctgtattg agttttttta atgctgcttc aagcaattgc cagcactggt catcagtgta
     6781 gttattgtac atgccaattt ttgtctggat aatcagtttt tccctgggga aattctttat
     6841 tgctctgcct attgcttctt cactctgacc attatgatag aagtgatggc tgtcaataaa
     6901 attcactccg tgctcaattg cgtatctgat aatctctgcg gctttctcaa aatccacagt
     6961 gccatcaggc aacatcggca acctcatggc accaaatcca aggattgaaa cattgtatga
     7021 ggtgttacca aattttctgt attgcatcct ttatcctttt ggttattaaa tttttgaaaa
     7081 ataggttaaa cgtaaattcg cttgcaggat attttgagaa ctggtttgtt tttttcaatg
     7141 ccaaactttt gtgatatatg ccctccaata atccatataa ttttaccacg attttttccc
     7201 tcttcaatag cgagaggaat ttttgtaatg tcttttaatg tttttttatg tgtcttccag
     7261 tattttttga aactcacctg ttttcccatg ccaagtggaa cgaaacgctc atttctatca
     7321 ggttgcttca acatcagtgt tttttccact ttttctgcgt ttacatacgc aacaaggata
     7381 tcctgattgt tgagaaattc ttctgagtaa tcagtgtatt ctgtgacaat tttccatcct
     7441 gtttctggaa caattgtttc tccaggaatt ataatttgaa ccggtttttt caggatgatt
     7501 tctttttcat caattttttt gcatacactc actgtgtttc cattgcaagt tgcataaatt
     7561 ttaagtttgt taagaaaaca ttgtttcttc tgcaatatca atatacgcag attttccact
     7621 gttgcaaaat ctggcggata ggtacttcca gacattctga ttaaaatctg tcttaaaaaa
     7681 aatctctgga tggatacctg ttgtctaaca aacatccctc ttgaaaattc aatgcaacca
     7741 tttttctcaa cagcatattt ttttattttt ttttctgtga aagattcgag aaaagcatat
     7801 tcatcgccaa ggattttcgc tgttcttgat atatttttct tgtaagcggg attgattttt
     7861 tcaagaagag gcaacacctg gtgcctcaaa aaatttctcg cgaattttgt atccagattg
     7921 ctttcgtcaa tcatatatct gattttcttc ttttcaagat aggaaaggat ctgcttcttt
     7981 gatattgaaa gcattggccg caaaagtaca atatcatcca tccttgaaac aggttgcatc
     8041 ccgcaaagtc ctttc
//
LOCUS       DAORYU010000039         8010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_6471_length_8010_cov_4.570585, whole genome shotgun sequence.
ACCESSION   DAORYU010000039 DAORYU010000000
VERSION     DAORYU010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8010
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6471_length_8010_cov_4.570585"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..641
                     /locus_tag="PLW07_02150"
     CDS             12..641
                     /locus_tag="PLW07_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOL49357.1"
                     /translation="MERKGFTLIELLVVIAIIAILAAMLLPVLGKAREKARGAVCLSN
                     MKQISLAMKMYHEDFDHRIITKAGGNFQYCLYDLGYVKNWQVFRCPSDQRKVDWTRAG
                     GCFSYYLNAGEAGWIGAGADRGYTTGTYDSSKAIYVFEAPNWAWADNYAYGYQYRNAY
                     LNGEIGTHSGIVNIIWHDYHVAPVSVKEMISCAAPPSSHWGIWTLIATD"
     gene            661..1362
                     /locus_tag="PLW07_02155"
     CDS             661..1362
                     /locus_tag="PLW07_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOL49358.1"
                     /translation="MKRGFTLIELLVVIAIIAILAAMLLPVLARARMQAKMSTCLANL
                     KQIGLGVQLYLQDNNDYWYPYRRGGGPPTWTASSSVNGAWEWLGFLETMCDQGYLKGT
                     MIWNSGYYGDTVNHQLKNSTGVVHCPCGDEQLREGYGAGGYWADYGYNAYLGNPAQTP
                     YIRKLSQVRKPAEMSVLFESVGGNHLPTATWFDYLFDGNYNDRHPQIKLINTVFVDGH
                     AKALTKSEYIAAGSP"
     gene            complement(2195..2281)
                     /locus_tag="PLW07_02160"
     tRNA            complement(2195..2281)
                     /locus_tag="PLW07_02160"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2245..2247),aa:Leu,seq:cag)
     gene            complement(2251..3105)
                     /locus_tag="PLW07_02165"
     CDS             complement(2251..3105)
                     /locus_tag="PLW07_02165"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49359.1"
                     /translation="MNTLQIHHFFKNSVEKNRVCHAFIIHGGSSVARMESAYTAAKIL
                     ECEKSPEICGTCYSCKTISSNNYPDVHLLQPEKCNLSIDEVRNIQEQMYTKPYMGRYR
                     IFIIETDWMQAPAANSLLKILEEPPSYGIIIILARNNKNFLPTIVSRAINLPLNPEID
                     MPVNDKNTLKEITEMIEAAADKHWQTLFEAAGEIAKNLSREEIENLFDSLILMGRESL
                     LQNMERNQFRLPDNAYSGIIKINDYRVLAGILDKRQYLKFNVNIRIFLTTVALETSLP
                     KWRNWQTR"
     gene            complement(3062..3712)
                     /gene="tmk"
                     /locus_tag="PLW07_02170"
     CDS             complement(3062..3712)
                     /gene="tmk"
                     /locus_tag="PLW07_02170"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:NF014299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOL49360.1"
                     /translation="MKKRAIIVSFEGIDGCGKSTQAKKFVNHLKSQGFDVLFFKEPGT
                     TKIGKKLRKILLVKTDDIHPLTELFLYLAARNQLVAEQILPLLTQKKVIVLDRFMDST
                     VAYQGYGRGLPVELINLAHNIILKNIKPDITFVIDASASILHREVKKNPDRLEKSVKF
                     QEKVRRGYFNIHRSEPGRVKIIKRQSIEKTFAQIKNEWDKFVNEYPANTSFLQKLG"
     gene            complement(3709..4281)
                     /locus_tag="PLW07_02175"
     CDS             complement(3709..4281)
                     /locus_tag="PLW07_02175"
                     /inference="COORDINATES: protein motif:HMM:NF028439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M99 family metallo-carboxypeptidase C-terminal
                     domain-containing protein"
                     /protein_id="HOL49361.1"
                     /translation="MNGDILARLLVLVCVGIAFLALLIGFLVYQINRKKGAIGILLAI
                     VFTIITGYYSYLFFFPTGAVKVTQQGNVQSPRITGTSGPAPLMVAIETEDGNRLTAAD
                     GDYLDIKTDMKIKIIGVVQNGNPVENVRVNVIGFAPKDNPQTINDTGYLFSYNDMLKK
                     FAVDDEKITYRVEVKQNDAKLAEIFLRFVK"
     gene            complement(4278..5516)
                     /gene="hisD"
                     /locus_tag="PLW07_02180"
     CDS             complement(4278..5516)
                     /gene="hisD"
                     /locus_tag="PLW07_02180"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOL49362.1"
                     /translation="MLQIRPVDVEKVLKERKLFSTSVENIVSKIISDVQKRRDGALRY
                     WTKKFDGVDIDNFQISEQEITSSVSKISKSDRQLIKQAIKRITSYHKKQRIKQFVIKE
                     KEIKISFRVKPVNRVGLYIPGGTAPLVSTVLMTGLPAKIAGVKEIIACSPPSCNGSIH
                     PYIIAALDFIGVNKIFKVGGAQAIAAMAYGTETIPKVNVIAGPGNIYVNAAKKILAGI
                     IGIDLLAGPSELVVLIDSSANPDFVIADLNAQEEHKQSMIFLISEDSAIARKISNNVK
                     AGYWIKIDTMESAIDIVNTIAPEHLQIVCKNANAIAEKVIAGAIFIGSYTPCAIGDYI
                     AGPSHTLPTGGSAIFDSGLNVFDFMRSYAVIEAQSQFFKKHGIIIERLAEIENLLNHK
                     MSVEIRRKKLKKTSILEREK"
     gene            complement(5504..6151)
                     /gene="rpe"
                     /locus_tag="PLW07_02185"
     CDS             complement(5504..6151)
                     /gene="rpe"
                     /locus_tag="PLW07_02185"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOL49363.1"
                     /translation="MMISPSLLSADFANLGKEVSAVTQSGADMIHFDVMDGHFVPNIT
                     FGPMILAAIRKYTHLPFEAHLMISHPEKYWKQFSDAGADIIGFHIEANIDHNHLIKEI
                     HAAGKKACIVINPPTEISSLEKVLSSVDIVLVMTVNPGFGGQKFISSNLKKIEWLDSQ
                     RKKNNYNFLIEVDGGINYETAPLVKKAGTDILVAGSFIFGSSDYKKTIENLKCCK"
     gene            6243..6701
                     /gene="smpB"
                     /locus_tag="PLW07_02190"
     CDS             6243..6701
                     /gene="smpB"
                     /locus_tag="PLW07_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005815658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOL49364.1"
                     /translation="MKKSIKIENRKALHDYAVLETIEAGIELKGPEVKSVRQGSVSLR
                     DSYAGFEKNELFLYKTHISQYPGAFEKIDPLRKRKLLLHRSQLVRLQRKVEEKGLTIV
                     PLSLYTNNKGIIKVNIALVRGKKLFDKRRDIKEKEVKRHIERQKRDTGAK"
     gene            6689..7035
                     /gene="ssrA"
                     /locus_tag="PLW07_02195"
     tmRNA           6689..7035
                     /gene="ssrA"
                     /locus_tag="PLW07_02195"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            7071..>8010
                     /locus_tag="PLW07_02200"
     CDS             7071..>8010
                     /locus_tag="PLW07_02200"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOL49365.1"
                     /translation="MRLIVKILVCVFFFSSLCFSSEFTNQIKNYPTYWIGKQKYVSLQ
                     TIIKILGIQSWGRIEDRIFIEYQGKILKGSIGSSDFYVDDKRMNLEYPVKEIEKEIFI
                     PVEPFDRILTTTYGSQLQTRVQKPDSQPAAASEIKPSGTEKSYGDKRFVILIDPGHGG
                     KDNGAVGNFGLKEKDVNLDISLRLKNFLVSQLKKNPNIVIKMTREDDRFLSLDERVEM
                     AKRENADIFFCVHTNSSRLNRWNADGFETYYPSRKSEISFTAKQETEELVETETSTEV
                     IFQIVSDLNTTSVIEESKILAEMVQEKLAERLICPDR"
BASE COUNT         2211 a         1604 c         1720 g         2475 t
ORIGIN      
        1 aggtggagaa aatggaaaga aaaggattta ctttaatcga gttgctcgtg gtgatagcaa
       61 tcattgcgat tcttgccgca atgttgcttc ctgtattagg caaagccagg gaaaaggcgc
      121 gtggtgcggt ttgtttgagc aacatgaaac agatttcgct tgccatgaaa atgtatcatg
      181 aagattttga ccacagaatt attactaagg caggtggtaa ctttcagtac tgtctgtatg
      241 accttgggta cgtgaaaaac tggcaggttt tcaggtgtcc ttctgaccag agaaaggtgg
      301 actggacgag agctggcggc tgtttttcct actatttgaa tgctggagaa gccgggtgga
      361 ttggcgctgg cgctgatagg ggatatacga caggaaccta tgatagttca aaggcaattt
      421 atgtatttga ggcaccaaac tgggcgtggg cagataatta tgcctatggg tatcagtaca
      481 ggaatgcata tttgaatggt gaaataggga cgcattcagg tatagtgaac atcatctggc
      541 acgactatca tgttgctcct gtttctgtga aggagatgat ttcgtgcgcg gctcccccca
      601 gcagccactg gggtatctgg acacttattg ccacagatta aaaatgaaag gaggtgaaaa
      661 atgaaaagag gatttacact catagaatta cttgtagtca tagcaatcat cgcaatcctt
      721 gctgcgatgt tgcttcctgt gcttgcaagg gcaaggatgc aggcaaagat gtccacttgc
      781 cttgcgaacc tgaagcagat aggcctcgga gtacaacttt atctccagga caataatgat
      841 tactggtatc catacagaag gggaggcgga cctcccacat ggactgcgtc ttcaagtgtc
      901 aatggtgcct gggaatggtt aggatttctt gaaacgatgt gtgatcaagg atacctgaag
      961 ggtactatga tctggaatag cggttattat ggcgatactg taaatcatca gcttaagaat
     1021 tccactggtg ttgttcactg tccgtgcgga gatgaacaat tgcgggaagg ctatggcgct
     1081 ggcggatact gggctgatta tggttacaat gcataccttg gtaatccggc tcaaacaccc
     1141 tatatccgaa aattgagcca ggtaagaaaa cccgctgaga tgagtgtgtt gtttgaaagc
     1201 gtaggcggaa atcatcttcc gactgctacc tggttcgatt acctttttga cggtaattac
     1261 aatgacagac acccgcaaat taagttaatc aatactgtgt ttgttgatgg acatgcaaaa
     1321 gcattgacga aatccgagta tattgctgct ggtagtcctt aatagttggg atagtagccg
     1381 ctcatacacg attgaagtgc gtggtaatag caaggattga aagagggatt gggagagatt
     1441 tcggataacc ccttccttag aaaaggaatg gttgagtgag tttccgaaac ctttctcaat
     1501 ctttcttaat tcttcatacc ataatcccta tcatgggcat caattttatg aagtttgcgt
     1561 tagggatggc tttgtccttg attcgtgaga attttccaac ttcagaactt ttcattcaaa
     1621 attttccatt tactccctct tttcccttcc tttgttctcc ccatttaata aaatccctct
     1681 tcctattctc ccccttttca aactcctctc ccggtcttcc ccctttacaa aagggggatt
     1741 gagggggatt tgaaatctcc cccagcccct cttttataaa gaggggagct attttttccc
     1801 ttgtaaaatt tccactctca ttctcccctt ttcaaatccc tctcgtcctt tccccctttg
     1861 taaaaggggg attaaggggg atttcaaaaa gaaaggagaa atctccccca gcccttcttt
     1921 ttcctcctct tgtttttccc cctttacaaa agggggatta agggggattt tgaaatctcc
     1981 cccgacccct cttttataaa gaggggagga attgggaaat ttataaatga tgaagatttg
     2041 aagttagaga gtagtgtttg gtttgttatt ggtgccgaag cggggatttg gcgccctcgt
     2101 ctcccactac gactcgggtc ggccagccgc acaggttctc gcttgctcga acctatgcgt
     2161 cttcgctcca ttcaaatccc cgtgtgattg attttggtgc cgaagcgggg atttgaaccc
     2221 cgacggcgtt gccaccacta ggtcctgaac ctagcgcgtc tgccagttcc gccacttcgg
     2281 caaggaagtt tccagcgcta ctgtcgtaag aaatattctg atgttcacat tgaatttaag
     2341 atactggcgt ttatcaagga ttcctgccag tactctataa tcgttgattt ttattatacc
     2401 cgaatatgcg ttgtctggca acctgaattg atttctttcc atgttttgca gcaaactttc
     2461 ccttcccatc aaaatcaaag aatcgaacag gttttcaatt tcttcccttg agagattttt
     2521 tgctatttca ccagctgctt caaaaagggt ttgccagtgt ttatcagcgg ctgcttcaat
     2581 catttcagtg atttctttca gtgtgttttt atcattgact ggcatgtcta tttcagggtt
     2641 taatggcaga ttgattgccc gggatactat cgttggaaga aaatttttgt tgttgcgggc
     2701 aagaattatg atgatgccgt aggaaggggg ttcttcaagg atttttaata aactattcgc
     2761 agcaggcgct tgcatccagt cggtttctat gatgaaaatt ctgtacctgc ccatgtaggg
     2821 ttttgtgtac atctgttcct gaatgtttct cacttcatca atggacagat tgcatttttc
     2881 tggttgcaac agatgaacat cagggtaatt gttggatgaa attgttttgc atgaataaca
     2941 tgttccacag atttcaggcg atttttcaca ttcaagaatt tttgcggcgg tataagcgct
     3001 ttccatccgt gcaaccgatg aaccgccatg gatgatgaat gcgtgacata ctctgttttt
     3061 ctcaaccgag tttttgaaga aatgatgtat ttgcagggta ttcattgacg aatttgtccc
     3121 attcattctt gatttgtgca aatgtttttt caatactctg cctcttgatg atttttaccc
     3181 ttccaggttc tgatcgatgg atgttgaagt atccacgtct gactttttcc tgaaatttta
     3241 ctgatttttc gagacggtca ggattttttt tcacttccct gtgcagaata gaagcagacg
     3301 cgtcaataac aaacgtgatg tctggtttta tgtttttcaa aataatgtta tgtgcaagat
     3361 tgatcaattc tactggaagt cctctaccat atccctgata ggcgactgtg gagtccataa
     3421 atctgtcaag cacaataacc tttttttgtg tgagaagagg caatatttgt tcggcaacca
     3481 gttgatttct tgctgcgaga tacagaaaga gttctgtcaa tgggtgaatg tcatctgttt
     3541 taacgagcaa aatttttctg agtttcttcc cgatttttgt tgtgcctggt tctttgaaaa
     3601 acagaacgtc aaatccctgc gattttagat gatttacgaa tttttttgcc tgcgtggact
     3661 ttccgcatcc atctattcct tcaaaactga cgatgatagc ccttttcttc atttaacaaa
     3721 tcttaaaaaa atctctgcca gttttgcgtc gttttgtttg acttccaccc tgtatgtgat
     3781 tttttcgtca tccacggcaa actttttcaa catgtcatta tatgaaaaca ggtatcctgt
     3841 atcattgata gtttgtggat tgtctttcgg cgcaaaacct atcacattga ctcttacgtt
     3901 ttcaacagga tttccatttt gaacaacacc tataatttta atcttcatat cggttttgat
     3961 gtcaaggtaa tcgccatctg ctgcagtcaa acggttgcca tcctctgttt ctatggcaac
     4021 cataagagga gcgggtcctg aagttccggt aatccgtggc gattgaacat ttccctgctg
     4081 tgtaactttt accgcgcctg tgggaaaaaa gaaaagataa ctgtaatacc ctgtgatgat
     4141 tgtgaacaca atggcaagca atatacctat ggcgcccttt tttctgttaa tctgatatac
     4201 aagaaaccca atcaatagcg cgagaaatgc aataccaaca cagaccagca ccagcaatct
     4261 tgcaagaatg tctccattca tttttccctc tccagtattg acgttttttt cagtttcttt
     4321 cttcttattt caacggacat tttatgattt agaaggtttt ctatttcagc aagtcgctca
     4381 ataatgatac catgtttttt gaaaaattgc gactgtgcct caatgactgc gtaacttctc
     4441 ataaaatcaa acacattgag cccggaatca aatatggcac ttccgcctgt tggaagggta
     4501 tgactgggtc ctgcaatgta gtcaccgatg gcacatggag tataacttcc tataaatatc
     4561 gctcctgcaa tcaccttttc tgcaatggcg tttgcatttt tgcaaacaat ctgcaggtgt
     4621 tctggcgcaa tggtattaac gatgtcaatg gctgattcca ttgtgtcaat tttgatccag
     4681 tatcctgcct taacattgtt cgaaattttt cttgcaatgg ctgaatcttc tgatatgaga
     4741 aatatcatgc tttgtttgtg ctcttcctga gcatttaaat ccgctatgac gaaatcagga
     4801 tttgccgaag aatcaatcaa aacgacaagt tcgctcggac cagcgagcaa atcaatacca
     4861 attattcctg caagtatttt ttttgccgca ttcacataga tgtttcctgg acctgctatg
     4921 acatttacct ttggaatggt ttcagttcca taagccattg ctgcaattgc ctgagcgcct
     4981 ccgaccttga atattttatt gacgccaatg aaatctaatg ccgcgattat gtatggatga
     5041 atacttccat tgcacgaagg tggactgcac gcgataattt ccttgactcc tgcaattttt
     5101 gcaggcagcc ccgtcatcag cactgttgaa acaaggggcg ctgttcctcc gggaatgtac
     5161 aggccaaccc tattcacagg tttaaccctg aaactgattt taatttcctt ttctttgatg
     5221 acaaattgct tgattctttg ctttttatga taactggtta ttcttttaat tgcctgtttg
     5281 attaattgtc tgtcggactt agaaatctta ctaaccgatg acgtaatttc ctgttcactg
     5341 atttgaaaat tgtcaatgtc tacgccatca aatttttttg tccagtacct taatgcgcca
     5401 tcgcgacgtt tttgaacatc agagatgatt tttgacacta tattttccac agaggtagaa
     5461 aacagttttc tttcctttaa caccttctca acatcaacag gtcttatttg caacatttca
     5521 ggttctctat tgttttttta tagtcgctgc taccaaagat aaacgagcca gcaacaagga
     5581 tatctgtgcc tgcctttttt acaagaggag ccgtttcata attgataccg ccatcaactt
     5641 ctatcagaaa attataattg ttcttttttc tctgtgagtc aagccactcg atttttttga
     5701 gattggacga gatgaatttc tgaccgccga atccaggatt tacagtcatc acaaggacga
     5761 tgtcaacact ggaaagcact ttttcaagcg atgaaatttc tgtgggtgga ttgattacaa
     5821 tacatgcctt ttttcctgcg gcatgaattt cttttatcag gtggttgtgg tcaatgttgg
     5881 cttcaatatg aaatccaatg atgtcagcgc cggcatctga aaattgtttc cagtattttt
     5941 caggatggga tatcatcagg tgcgcttcaa atggaagatg cgtgtatttt cttatggctg
     6001 caagaatcat gggcccgaaa gtgatatttg ggacaaaatg accatccatg acatcaaagt
     6061 gtatcatgtc agcgcctgat tgggtgacag cgctcacttc tttcccaaga tttgcaaagt
     6121 ccgctgataa tagagaagga gatatcatca tgcatctaat tataaagaac ttgcatcgtt
     6181 cagtcaaatt gcatattgcc ttgagaattg acgtaactgg ctgtatgtgt ttaattatct
     6241 ccatgaaaaa gtcgataaaa attgaaaaca ggaaggcgct tcacgattat gcggttcttg
     6301 aaacaatcga ggcagggatt gaactgaaag gacctgaggt taaatcagta aggcagggta
     6361 gtgtaagttt gcgggattct tacgcaggat ttgagaaaaa tgaacttttt ctttataaaa
     6421 cgcatatctc ccagtatccc ggagcatttg agaaaataga cccgttaagg aaaaggaaat
     6481 tgctccttca ccgcagtcaa cttgtacgat tacagagaaa agtcgaggaa aaaggattga
     6541 caatagtccc gctttcgctg tatactaata ataaaggcat aattaaagta aatattgctc
     6601 ttgttcgtgg gaaaaaactc tttgacaaac gtcgggacat taaggaaaaa gaagtaaaac
     6661 gtcacattga aagacaaaaa agagatacgg gggcgaaata ggtctcgaca ggaggcagga
     6721 gtgagaaaag ctgccgaccg agtcgccggg ggctcgtaaa aaccggcaaa caaaatagct
     6781 gccacaacag ctaactgtct ggctgcataa gcagccacct gacagaatcc ctgccctcgg
     6841 gattctgacc caggtcgcat gagggctcgt ttcagtttcc ctggtcccag gaaactggaa
     6901 tcagaaacaa tggggcaggt attctggaat cctgtttcgg ggagtccaga attacccaat
     6961 aaaaccgaaa ctatgccggt agatgctttt ctacttccct tctggacgcg ggggcagtac
     7021 ccgccgcctc caccttctga tgaaaaatca tttaattaac aggagtgttc gtgagattaa
     7081 tagtaaaaat tcttgtttgt gttttctttt tttcctctct gtgtttctct tcagaattca
     7141 caaatcagat taaaaactat ccgacctact ggattggcaa gcaaaaatat gtgagtttac
     7201 agacaattat caaaattctg ggaattcaga gttggggaag aatagaagac aggattttta
     7261 ttgaatatca gggcaagatt ctcaaaggaa gtattggttc atctgatttc tatgtggacg
     7321 ataaaagaat gaatcttgaa tatccggtca aagaaatcga aaaagaaatt tttattccgg
     7381 ttgaaccgtt tgatagaatt ctcaccacta cctatggttc acaacttcaa acccgggttc
     7441 agaagccaga ttctcaacca gcagccgctt cagagatcaa accatccggt acagaaaaat
     7501 catacgggga taaaagattt gttattctta ttgatccggg gcatggtggt aaagacaatg
     7561 gcgctgttgg taattttgga ttaaaagaaa aggatgtcaa tcttgatata tcgttgagat
     7621 tgaagaattt tcttgtcagc cagttgaaaa agaacccgaa tattgtgata aaaatgacaa
     7681 gggaggatga caggttttta tctcttgatg aacgggttga aatggcaaaa cgtgaaaatg
     7741 ctgacatatt tttctgtgtt catacaaatt cttcacgatt gaacagatgg aatgcagatg
     7801 gatttgaaac atattaccct tcaagaaaaa gcgaaatctc attcacggca aaacaggaaa
     7861 cagaggaatt ggttgaaact gaaaccagta cagaggtgat ttttcaaata gtgtcagacc
     7921 tgaacacaac gtcagtcatt gaggaaagca aaattctggc tgaaatggtg caggagaaac
     7981 ttgccgaacg attgatatgt cctgacaggg
//
LOCUS       DAORYU010000040         7981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_6493_length_7981_cov_4.119228, whole genome shotgun sequence.
ACCESSION   DAORYU010000040 DAORYU010000000
VERSION     DAORYU010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7981
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6493_length_7981_cov_4.119228"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..1323)
                     /locus_tag="PLW07_02205"
     CDS             complement(91..1323)
                     /locus_tag="PLW07_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease Q family protein"
                     /protein_id="HOL49366.1"
                     /translation="MMYIADFHIHSKYSRATSKNMDIEHLTRWAKNKGISLLGTGDIT
                     HPLWFAEIRKKLQPSEKEGIYTFDGIDFILSGEVNNIFTKNKAVRKIHNIVFFHSFEG
                     AEKFNKNLEKFTELLIDGRPILQIDARTLVKMVRDADPEGFVVPAHIWTPHFSLFGAN
                     SGFDRIEDCFEEETSNIFALETGLSSDPPMNWLLSMLDQFMLISNSDAHSPQKIGREA
                     NVFSESFSFLELKKILKTQDKTRFLFTIEYFPEEGKYHYDGHRLCKVRLSPEETLKNR
                     LLCPVCGKKVTVGVMHRVTTLADRKNQNQPANRIGYKHLVPLDQIIASVYQKDTEANF
                     TTQMYAQVTGKLGPELKILLEIPEQELFANLPKELATGIKLVREEKLVVIPGYDGEFG
                     TVKINPLLSESQVQQTLF"
     gene            complement(1323..1823)
                     /locus_tag="PLW07_02210"
     CDS             complement(1323..1823)
                     /locus_tag="PLW07_02210"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOL49367.1"
                     /translation="MEQKPVKILWAPWRINYIKKGNNKRCFLCEISHKKRNKFLLWKG
                     DYSMVVMNIYPYNNGHLMIAPIKHKKDFNSLQNDEIGEMFHILKTTVNILNKTLKPQG
                     FNIGINLGRCAGAGLPGHIHIHIVPRWNGDTNFMPVLASTKIISQSLEELSDILKKEF
                     SKIKKG"
     gene            complement(1804..3201)
                     /locus_tag="PLW07_02215"
     CDS             complement(1804..3201)
                     /locus_tag="PLW07_02215"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOL49368.1"
                     /translation="MTVAPEVIIEQIKSLGNKSGITPFLVGGYLRDKFLRRRSHDIDI
                     MVETDALEFARLFAKTYGYPSPIFYGRFGTAMIEIDNNKLEFATARKESYEENSRKPH
                     VKPATVIEDLARRDFTINAIALNLLKSQILDPFDGKKDIRKKIIRTPVDPDKTFYDDP
                     LRILRGIRFATTLNFGIEEDTKKAMANNAKRLLIVSQERIADEIMKIISARKPSKGFF
                     LMDDIGVLELIIPEVAALKEKRTNHPCKELFEHTLQVLDNTASMTKSPVLRLAALLHD
                     IGKPRTLKVENGKVSFHRHEIVGAKMSIRVCGRLKISQKDTETIGLLIKHHLRPHLLA
                     KENPTDNALARFIREIGVNMKGLFIIARADITSKNEKKVKQGREKILALHERIKQVNK
                     KMRLARFKLALDGFNIMEILGIEPGKQVGVIKKNLEELVLSGKIENKKKALKKYLKEA
                     RKDILAGSGHGTKTS"
     gene            complement(3209..4903)
                     /locus_tag="PLW07_02220"
     CDS             complement(3209..4903)
                     /locus_tag="PLW07_02220"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOL49369.1"
                     /translation="MLTDLEIAQKAKIKDIREIAESAGIDEKFLELYGNHKAKVSLEI
                     MKQIEHNPAGKYIDVTAITPTPLGEGKTVTTIGLSQAIGKLKKKVFTCIRQPSLGPVF
                     GIKGGAAGGGYSQVIPMEDFNLHLTGDVHAVGLAHNLLAAFIDNSLLKGNPFNIDPLS
                     ITWPRVVDVSDRTLRHIIIGLGGKDNGIPRETGFDITVASEVMAILALTSDIFDLKKR
                     LSSIIIGTSRKGTPVTAVDLKASGAMTVLLKDAIKPTLMQTLEHTPVLVHAGPFANIA
                     HGNSSIIADKIAIRLADYVVTESGFGADCGFEKFANIKCRYSGLKPDCVVIVCSIRAL
                     KMHSGQFRVVPGKPLDKGLIEENLDAIEKGAKNLAKHIENVGLFGIPAVVAINRFTTD
                     TDREIELVRQLSLNAGAKAAEVSNVWAEGGNGGLNLAKKVLEACEEKHDFHFLYPLEK
                     PIKEKIEIIATRIYGAARVSYSQAAEEKIKLYTRWGFNNLPICMAKTHLSLSHDPNLK
                     GAPEGFTLPVEDVRLSAGAGFIYPLCGKMRTMPGLPTEPAGAHVDIDEKGRTVGLF"
     gene            complement(4926..5786)
                     /locus_tag="PLW07_02225"
     CDS             complement(4926..5786)
                     /locus_tag="PLW07_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HOL49370.1"
                     /translation="MAAQILAGKEIAEKIKQTVKKDLEQLKADGIIPKLVAVQVGENP
                     SSLVYLNAQKKSCQAMGIEHEVKNLDGNISESDLIQFIKNLNADRTTTGIILQMPLPA
                     GMDVRKVQSTISPEKDVEGVNPANLGWIVYGKPVLAPCTALAVKELIDSTGINLYGKE
                     VVMVGHSDIVGKPVALLLVDKFATVSICHIGTSDSGMLETHIKRAEILVVAVGKAHLV
                     KGSWIKKDAIVVDVGINKLGDKIVGDVEFDTAKENASWITPVPGGVGPVTTAILLRNT
                     VLAAKMQFSN"
     gene            complement(5806..6438)
                     /locus_tag="PLW07_02230"
     CDS             complement(5806..6438)
                     /locus_tag="PLW07_02230"
                     /inference="COORDINATES: protein motif:HMM:NF016825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HOL49371.1"
                     /translation="MEYKYLKEPLEKYIERLSEKSPCPGGGSAAILSLALANALILMV
                     CNYTVDSTKVEENSRKISKDILDNALQIQPLINKYVEQDSIIYKEIQETMKVCRKDPT
                     KATEYENALKKSINLHIQILDYCQSMLRWNEILVEHCNPYLISDVGVSASLIEGVIRA
                     TEVNILINLREIKNVQYVTEGTNKIQVLSSLIQKAIDIIRKVEMKISSGT"
     gene            complement(6462..7148)
                     /locus_tag="PLW07_02235"
     CDS             complement(6462..7148)
                     /locus_tag="PLW07_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49372.1"
                     /translation="MNRKKICKDALAILMVGMLFSGCAREKKPTVNIDEKLLQEAIEY
                     GKVKAGMSNYEFMEPWSVYLGYEIGKGRVVYFTPFLQAAQLAKNAAEKNLKPDINIIK
                     KAIAAKSDTLNFLVLTYGNEPDTSKRMKVFLICDKTRIEPVFAHFPPFGEFNRDYYQQ
                     INGEVRFPRKDIPENATVKLCIEIFPKMEKDDKHHEDEHHHHESEIVVGQQTQDHIIT
                     EFVFNLSKYR"
     gene            7261..>7981
                     /locus_tag="PLW07_02240"
     CDS             7261..>7981
                     /locus_tag="PLW07_02240"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223059.1"
                     /note="catalyzes the formation of 2-isopropylmalate from
                     acetyl-CoA and 2-oxoisovalerate in leucine biosynthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOL49373.1"
                     /translation="MDRIIFFDTTLRDGEQSPGASLTSSAKLTIARHLARLNVDVIEA
                     GFPVASEDDAYAVKLIGKEVNGPAVCALARCVPRDVEVALKSLESARKPRLHLFLATS
                     EIHRKFKLVKAKSEIIRIARDMVKFAKKFIDDIEFSPEDATRTESDFLFEVCQTVVEE
                     GARTLNIPDTVGYAVPEEYGALIGFLREKFPPEIVISVHCHNDLGLAVANSLTAVKNG
                     ARQVECTINGIGERAGNASLEE"
BASE COUNT         2096 a         1614 c         1515 g         2756 t
ORIGIN      
        1 cataaaagcg acaacggata aaagaaaatc gcccagattc tggagacgat ccttccactc
       61 tgaattaatg tcagaagatc gtacagtttc ttaaaaaagg gtctgctgga cctgactctc
      121 agaaagcaga gggttgattt taactgttcc gaattctcca tcatatcctg gtataacgac
      181 aagtttttct tctctgacaa gttttatgcc ggttgcaagt tcctttggca aatttgcaaa
      241 aagttcctgt tctggtattt cgagtaaaat tttcaattca gggcccaatt tccctgtaac
      301 ctgcgcatac atctgcgttg tgaaatttgc ttccgtgtct ttttgataga cagaagcgat
      361 aatctgatca agtggtacaa ggtgtttata tccgattcta tttgcaggtt gattttgatt
      421 tttcctatct gcgagagtcg taaccctgtg catcacacca acggtcactt ttttcccgca
      481 tacaggacat aaaagtctat tttttagtgt ttcttctgga gaaagccgaa ccttgcaaag
      541 tctatgtcca tcatagtggt atttcccttc ttcagggaag tattctattg tgaataagaa
      601 tcttgtttta tcttgagttt tgagaatttt ctttagctcc aggaaagaaa atgattcaga
      661 aaacacattc gcttcccttc caattttttg tggtgaatgt gcatctgaat tggagatcaa
      721 catgaactga tcgagcatcg aaagcaacca gttcattgga gggtcactgc ttagtcctgt
      781 ttccagcgca aaaatattac tcgtttcttc ttcaaagcag tcctcaattc tatcaaaacc
      841 cgaatttgca ccaaaaagtg agaaatgagg agtccagatg tgcgctggca caacgaaacc
      901 ttctggatca gcgtctctaa ccattttaac aagtgttctc gcatcaatct gaaggattgg
      961 tctgccatca atgagcaatt ctgtgaattt ttccagattt ttattgaatt tttctgctcc
     1021 ttcaaatgaa tggaaaaaca cgatattatg aatttttctg actgccttat tctttgtaaa
     1081 tatattgttt acttcgcccg ataaaatgaa gtcaatgcca tcaaatgtgt atattccttc
     1141 tttttccgaa ggctggagtt ttttcctaat ctcagcgaac cacaatggat gtgtgatatc
     1201 accggttccg agtaaagaga tacctttatt ttttgcccag cgtgtaaggt gttctatatc
     1261 catattttta cttgtggcgc ggctatattt tgagtgaata tgaaaatctg caatgtacat
     1321 gattaccctt tcttaatttt tgaaaattct ttttttagta tgtcagatag ttcttcaaga
     1381 gattgagaaa tgattttagt tgatgcaaga accggcataa aattggtgtc accattccat
     1441 ctgggaacaa tatgtatgtg aatatggcct gggagccctg ctcctgcaca ccggccaaga
     1501 ttgatgccaa tgttgaaacc ctggggtttc aatgttttat tcagaatgtt aacagttgtt
     1561 ttaagaatat gaaacatttc accaatttca tcgttctgta aactgttaaa atctttttta
     1621 tgtttaatgg gtgctatcat gagatggcca ttgttataag gataaatatt cattacaacc
     1681 attgagtaat cacctttcca cagaagaaat ttgttccttt tcttatggct gatttcgcaa
     1741 aggaaacatc ttttgttgtt tcccttttta atgtaattta ttctccacgg cgcccaaagg
     1801 atcttaactg gtttttgttc catgacccga acctgccaaa atatccttac gtgcttcttt
     1861 tagatatttt ttcaaagctt ttttcttgtt ttcaatctta ccacttaaca caagttcttc
     1921 caaatttttc ttgattacgc cgacctgttt ccctggttca ataccgagga tttccattat
     1981 attgaatccg tcaagggcga gtttaaatct tgccagacgc atttttttgt tcacctgttt
     2041 gatccgttca tgcagtgcaa gaattttttc tcttccctgt ttcacctttt tttcgttctt
     2101 gcttgtgatg tctgctctgg caataataaa aagtcctttc atattcaccc caatttccct
     2161 gatgaatcgg gcaagagcat tgtcagtagg attttctttt gccagcagat ggggtctgag
     2221 gtgatgtttt atcaataggc caattgtttc tgtgtctttt tgtgagattt tcaatcttcc
     2281 acaaaccctt atgctcattt ttgcaccaac gatttcatgc ctgtgaaatg acaccttacc
     2341 gttttcgact ttcaatgttc ttggtttacc tatatcatga agaagggctg ctaatcgtag
     2401 cacaggactt tttgtcatgg aagcagtatt atcaaggacc tgaagtgtat gttcaaaaag
     2461 ttccttacat ggatggtttg ttcttttttc tttcaatgct gcgacttcag gaataattag
     2521 ttcaagtacg ccgatgtcat ccataagaaa aaatcccttt gatggttttc tagctgaaat
     2581 aattttcata atttcgtccg cgattctttc ctggctgact attaacaaac gttttgcatt
     2641 gtttgccatt gctttttttg tatcttcttc tatgccaaag tttaacgttg tcgcaaatct
     2701 tatgcctctg aggattctga gagggtcatc ataaaatgtt ttatcaggat ctacgggagt
     2761 tctgataatt tttttgcgaa tatctttttt cccatcaaat ggatcaagta tctggctctt
     2821 taataaattc agtgctattg cattaattgt gaaatcccgt cttgccagat cttcaatcac
     2881 agttgcgggt tttacatgag gctttcgaga attttcttca taactttctt ttcttgcggt
     2941 tgcaaattct aatttattgt tgtctatttc aatcattgct gtgccaaatc ttccgtaaaa
     3001 aattggtgat ggatatccgt atgtttttgc aaaaagtcgc gcaaattcaa gggcgtcagt
     3061 ttccaccatg atatcaatgt catgacttct tcgtcttaaa aatttatccc tgagataacc
     3121 gccaacgaga aaaggggtta tgccgctctt gttaccgaga ctttttatct gttctatgat
     3181 aacttcaggt gctactgtca tagatcacct aaaaaagccc aacagttctg cctttttcgt
     3241 ctatgtccac atgcgctcct gcgggttctg tgggtaatcc tggcatggtt ctcatctttc
     3301 cacagagggg atatataaaa ccagcgcctg ctgaaagccg tacatcttct actggtaggg
     3361 taaatccttc tggcgcacct ttgagattgg ggtcatggga caaagaaaga tgagtttttg
     3421 ccatgcagat tggtagattg ttgaatcccc agcgggtgta cagtttaatt ttttcttctg
     3481 ctgcttgtga ataagaaact cttgctgctc cgtaaattct ggttgctatg atttcgattt
     3541 tttcttttat cggtttttcg agcggataga gaaaatggaa atcgtgtttt tcttcacacg
     3601 cttcaagcac cttttttgca agattcaagc caccatttcc gccttctgcc catacattgc
     3661 tgacttccgc tgcttttgcc cctgcattca gagaaagttg tcttaccagt tcaatttccc
     3721 tgtcggtgtc tgttgtgaat ctattaattg cgaccacagc gggaattccg aaaaggccga
     3781 cgttttctat gtgttttgcg agattttttg cgcccttctc tatagcgtcg agattttctt
     3841 caatcagtcc cttatcaagg ggtttgccag gaacaaccct aaactgcccg ctgtgcattt
     3901 tcaacgctct tattgagcaa actatcacca cgcaatctgg tttcagcccg ctatatctac
     3961 acttgatatt cgcgaacttt tcaaatccgc aatctgcgcc gaaaccactt tctgtcacaa
     4021 cataatcagc aagacgaatc gcaattttgt ctgcaatgat agaactgttt ccatgtgcta
     4081 tgtttgcaaa tggacctgca tgaacaagaa caggcgtgtg ttcaagcgtt tgcatcagtg
     4141 ttggtttaat tgcatcttta aggagaactg tcattgcgcc ggatgctttc aaatctaccg
     4201 cagttacagg cgtacctttt ctggatgtcc cgataatgat gcttgataat cttttcttta
     4261 aatcaaaaat atcgctggtt agcgcaagga ttgccattac ctctgatgca actgtaatat
     4321 caaatccagt ttctcttgga atcccattgt cttttccacc aagtcctata atgatgtggc
     4381 gtagtgttct atcactgaca tcaacaactc ttggccaggt aattgagagg ggatcaatat
     4441 tgaaaggatt cccttttaaa agggaattgt caataaatgc ggccagaagg ttatgagcaa
     4501 ggccaacagc atgaacgtct cctgtgagat gcagattgaa atcttccatc gggataacct
     4561 gcgaatatcc accgcctgcc gcaccacctt taattccaaa cacaggtcca agtgacggtt
     4621 gacggatgca tgtaaaaacc ttttttttga gttttccgat ggcttgcgat agccctatcg
     4681 tagtgactgt ttttccttca ccgagggggg ttggtgttat tgcagtaaca tcgatatatt
     4741 ttcctgctgg attgtgttca atttgtttca taatttcaag agaaactttt gccttatgat
     4801 ttccatagag ttcaagaaat ttctcatcta ttccagcact ttcagcgatt tcacgaatat
     4861 cttttatttt tgctttctgc gcgatttcaa gatctgttag catgttttct cctttggaca
     4921 gaatattaat ttgaaaattg catttttgcc gcaagcacag tatttctcaa caaaattgcc
     4981 gtggtaacag gtcccactcc accaggaacc ggtgtaatcc aggaagcatt ttcttttgca
     5041 gtgtcaaact caacgtctcc cacaatttta tcccctaatt tatttattcc aacatccaca
     5101 actatggcat cctttttgat ccaggaacct ttaaccagat gggctttccc aacagctaca
     5161 acaagaattt ctgctctttt gatatgggtt tcaagcatac cactatcact ggttccaatg
     5221 tggcatatac tcacggtggc aaatttatca acaagcaaaa gtgctactgg tttgccaaca
     5281 atatcactgt gacctaccat cacaacttcc ttaccataca gattaatacc ggtggaatca
     5341 attagttctt ttacggcgag tgcggtacag ggagcaagaa caggtttgcc atatacaatc
     5401 caccctaagt ttgctggatt aacaccttcc acatcttttt ccgggcttat ggtggattga
     5461 acctttctca catccatacc cgcaggcagt ggcatttgta aaatgatccc tgtggttgtt
     5521 cgatctgcat tgagattttt tataaactga attagatctg attctgaaat attgccgtca
     5581 agatttttta cttcgtgttc tatgcccatt gcctgacagc tttttttctg tgcattgaga
     5641 taaacgagag atgatggatt ttctccaacc tgcacggcaa caagctttgg aatgatccca
     5701 tctgctttca attgctcaag atctttttta actgtttgct taattttttc tgcaatttct
     5761 tttcctgcaa gaatttgagc agccatcttt cctccgtttt ttggtttatg tgcctgaaga
     5821 aattttcatt tcaacctttc ttattatatc aatggctttt tggattaatg aagataaaac
     5881 ttgaattttg tttgtgccct ctgtgacata ttgaacattt ttaatttctc tcaggttgat
     5941 tagaatattt acttctgttg cccttatcac gccttcgatc agtgatgcag agacaccaac
     6001 atcgcttatc aaatatgggt tacaatgttc cacaagtatt tcgttccacc gcagcatgga
     6061 ttgacagtaa tcaagaatct gaatatgtaa attaatactc tttttcagtg cattttcata
     6121 ttctgtcgct tttgttgggt cttttcgaca aactttcatt gtttcctgaa tttctttata
     6181 aatgatgcta tcctgttcaa catatttgtt tatcaatggt tgtatctgta acgcattgtc
     6241 cagaatgtcc tttgagattt tcctggaatt ttcttccacc tttgtactgt ccactgtgta
     6301 attacatacc atcagaatta acgcatttgc cagggcaagg gataaaattg ctgcgctgcc
     6361 accaccaggg cacggtgatt tttcagaaag acgttcaata tatttttcta aaggttcttt
     6421 taaatattta tattccattt ggccttgttc agtatgaaga actacctgta ttttgagagg
     6481 ttaaatacga attctgtgat aatatgatcc tgtgtttgtt gcccaacgac tatttctgac
     6541 tcatgatggt gatgttcgtc ttcatgatgt ttgtcgtctt tttccatctt ggggaagatt
     6601 tcgatacaca atttgactgt ggcattttca ggaatatctt ttctcggaaa tcttacttct
     6661 ccattaatct gttgataata atcacgatta aattctccaa agggtggaaa atgagcaaat
     6721 actggttcta ttctcgtttt atcacatatg aggaatacct tcattctttt tgatgtgtct
     6781 ggttcgttgc catatgtgag gaccaaaaaa ttaagtgtat cactttttgc agcgattgct
     6841 ttttttatta tgtttatgtc aggtttgagg tttttctctg cagcattttt tgccagttgt
     6901 gcggcctgta gaaatggggt aaagtacacc accctgccct tgccaatttc atacccgaga
     6961 taaacactcc atggttccat aaattcatag ttggacatac ctgcttttac tttgccatac
     7021 tcaatggctt cctgcaataa tttttcatca atatttactg tgggtttctt ttctcttgcg
     7081 cacccggaaa aaagcatacc gaccattaaa attgcgagag cgtctttgca gatttttttt
     7141 ctattcatga gtataaatat attcattttt gctttttctg tcaagtaagg gtaaaataaa
     7201 aatctaataa aactggtagt acagaagtta taaaaactac tgcgtttgaa gggaaaggtt
     7261 atggatagaa taatattttt tgacacgact ttaagagatg gcgaacagtc ccctggagcc
     7321 agtcttacca gtagtgctaa actgacgatt gccaggcacc tcgccagatt aaatgttgat
     7381 gtaattgaag caggttttcc tgtggcttca gaagatgatg cgtatgcggt aaaactgatt
     7441 ggaaaagaag ttaatggccc tgcggtttgt gcccttgcgc gatgtgtccc cagggatgtg
     7501 gaagtcgcat tgaaatctct cgagagtgcc aggaaacccc gattacatct ttttcttgcg
     7561 acatcagaaa ttcacagaaa gtttaaactg gtaaaagcta aaagcgaaat aataagaatt
     7621 gccagggata tggtgaaatt tgcgaaaaag ttcattgacg acattgaatt ttctccagaa
     7681 gatgcaacca gaactgaaag cgattttctt tttgaggttt gtcagaccgt tgttgaagaa
     7741 ggtgcacgaa cactcaatat tcccgacacg gttggatatg ctgtgcctga ggagtatgga
     7801 gcattgatcg gatttctgag agaaaaattt ccaccagaaa tagtgatcag tgtgcactgt
     7861 cataacgacc tcggacttgc ggttgcaaat tcacttactg ctgtgaaaaa tggcgcaaga
     7921 caggttgagt gcacgattaa tggtattggt gaacgggcag ggaatgcatc tcttgaagaa
     7981 a
//
LOCUS       DAORYU010000041         7955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_6522_length_7955_cov_4.460380, whole genome shotgun sequence.
ACCESSION   DAORYU010000041 DAORYU010000000
VERSION     DAORYU010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7955
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6522_length_7955_cov_4.460380"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="PLW07_02245"
     CDS             complement(<1..856)
                     /locus_tag="PLW07_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008120459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HOL49374.1"
                     /translation="MADFTLKEILKLHNDGKLGISMKVPLKTKKDLSIAYTPGVAEVC
                     KIIEKNPELAYQYTIKKNSVAVVTDGSAVLGLGNIGGLAALPVMEGKAILFKEFGGVD
                     AYPVCLNTQNTKKIIQIVCAIAPGFGGINLEDISSPRCFIIERELKKKLNIPVFHDDQ
                     HGTAIVVLAAIINSLKLAGKKPGSVRVVINGAGAAGIAVTKILYGYGIRNIVMCDRHG
                     IVCRERKTGMNSAKKEILRYLMKSRPGTLADALEDADIFIGLSGPNLVSPEMVKKMKA
                     NPIIFAMAN"
     gene            complement(861..1424)
                     /gene="pth"
                     /locus_tag="PLW07_02250"
     CDS             complement(861..1424)
                     /gene="pth"
                     /locus_tag="PLW07_02250"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOL49375.1"
                     /translation="MVILGLGNPGKSYLNNRHNVGVKVISELISRSRLIKSWKRKYFR
                     ACEIEICGRNVIVAKPSTFMNESGKAALALCTGFGLKPDEILLVYDDINLALGKIRIR
                     KKGSSGGHKGVQSVIEAIGTEKFPRIKLGIGIPSENMDLVDYVLSDFHEDEKPIAQQM
                     IKDAAEAIELVLNEGIEKAMAVFNGKG"
     gene            complement(1428..2147)
                     /locus_tag="PLW07_02255"
     CDS             complement(1428..2147)
                     /locus_tag="PLW07_02255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOL49376.1"
                     /translation="MTEKDKKQVKFVAQMRNSAGKSESRALRRNGFIPGILYGAGEEN
                     KLLAVDKKSAEIVLKHVASHSVMADLEIQSNEGKNILKTIVKEVQSNPITGEVLHIDF
                     YHIGKNQPVSLSIPIRLIGECPGVKEGGIMEHDLREIEVEGLPDAIPEAVEIDVSNLK
                     IGDSIYVRDMTFPEGIKPLVDSEHVVVTILAPKAEEVPAPEEAAAVATEEVQQPKVIT
                     QEIAEERRKEKEAKKEAEKGE"
     gene            2244..3116
                     /locus_tag="PLW07_02260"
     CDS             2244..3116
                     /locus_tag="PLW07_02260"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HOL49377.1"
                     /translation="MEIFFRKDLLEYREPEHPECPERLEAIANFLEKKGKNQFHPFQQ
                     IPESLLLEVHSQRLVNMVRNNTFFDPDTPNIQNIYTYACLSAGGAVAAAQSALDGKDA
                     CALIRPPGHHAGKDFLGGFCYFNNVAIAVQYCMKKVKKIAILDIDGHHGNGTEAVFKN
                     VDNVMFISIHQYPAYPGTGLASFSNCYNFPVKPNTAFSDYRIFLVEAMGLIKRFAPDI
                     LGISLGFDTHLDDPLLQLRFVDSDYYHMAKMIASGISCPVFIVFEGGYDTVSIGNSFF
                     CFLSGWEKTKNGAG"
     gene            3177..4958
                     /locus_tag="PLW07_02265"
     CDS             3177..4958
                     /locus_tag="PLW07_02265"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HOL49378.1"
                     /translation="MKNIRVVFEPGMIEVDVPEGSKIISAIERSGIPFELPCGGKGIC
                     GKCGVIVLEGASTPTETEKKLLGGKVKSGWRLACQSRIYHRCKILIEGKSSSYHKILT
                     TKVLCDMPRNPQITKKHIVLPQITLDNPVSLQEQMAMFLGMGIEQVSPLLLRDPSLYE
                     GNDFTLVFCNGNIISIEKGDTTDQIYGIGFDLGTTTMVLTVFDLVNCREIITVVRPNP
                     QIKFGDDVISRIDFSLKESGLNTLCRVVVDEINKMIDEAVKTAGIKRQHIYQFVLSGN
                     TVMEHIFLNLPLKTLSRIPFNPVMKGPVEFPAYTSGLHMNPEGKLFVFPILGGFVGGD
                     TTGLILATGIHKSEKIQVGIDIGTNGEIVIGNKDGIIAASTAAGPAFEGGRITFGMRA
                     QIGALEKCWFSDGKLQWQIIGGNSLKGICGSGLVDLITVLRQQGIIDETGRFDENSSS
                     QLAELLKSRDGNQLFNLENDNQSIYLTQKDVREFQAAKAAIRTGVEILMAIRNVKIDD
                     IEHVYLAGALGNFVNIENIKNLKMLPDFRAETIVPSGNTSLASTLLFLCNADLRKDIE
                     YIAGITKTVELSLYPSFQDIFTEALLF"
     gene            4962..6779
                     /locus_tag="PLW07_02270"
     CDS             4962..6779
                     /locus_tag="PLW07_02270"
                     /inference="COORDINATES: protein motif:HMM:NF036997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49379.1"
                     /translation="MNNQQLVSIAIDIPARTNFTYKTDILLKPGQRVLVPFGKRKMLG
                     WVIGPGVEGPYQYKKIFKVYDEYPLLPQHLLNLAFEIAHMYFCSVGSVLAMMSKNLSL
                     KKIDTEIKSVSGTKTPDFNNEIADRIFEDINKTGRKFFVCQFNRMEEKIKFFQALPTK
                     IQGSCAFVISNYFDAKKHADTLKNIFGDRVIFFTGESSKTEKVFLWHRMLNEKNLIII
                     GTRLALFTPVSDLEILIVDEPSEYGHKENQTPRYTSREVATKISQLLGIPVVFTVFQP
                     DVTDIFLLKTKKAVLIEAQQRNFPRVVISRMQGKKHTDILTDTAKHFLEKSVLEKGKV
                     VIIHNLKGYARLVICKSCGASVLCQECGSPVCAVNENFTFCTKCKKIFPISKKCFVCK
                     KATLSMRRLGIQKIAKMLKTMYPDFDVGVQDDGDTIDMNRQIIIGTQHLVSHVHDISP
                     SLLIFTDADMIAARSAFRSEEKFFLLVEKIKRMMEPQHMIIIQTRNPGLELYTDVVNN
                     SHESFYSRELAIRNSLMFPPYGDFIEIGVSGKHWKKNKDEIFEKLKKSGEIYEVSEKM
                     KKTVFLWKITERNNAFEILKNVLEKYRIHNYSIDINPYF"
     gene            complement(6776..>7955)
                     /locus_tag="PLW07_02275"
     CDS             complement(6776..>7955)
                     /locus_tag="PLW07_02275"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49380.1"
                     /translation="SFFKKTKKHWDVIIHDAGLPFSLKIANFYTKEFYNLVKTHLSQD
                     GIFYTLLPGSPEYMSEPLADVHNITYATLKNVFPSVHVIPGYFTGYCAAVNSNMPEFS
                     SSFIISQSKKKQIQTGLVTDFYVSDRLDTGKRHHFLEMISTDKILINTQLKPIIIIPA
                     IKYWISMSESGKKSCDMNHYVTISIVLLTIFLVCTIIYVVKTPTKTRILELTMFSTGL
                     LSIAWELIYLFLFQINFGSVYFYLSILTGVFMGGMAAGSIIFPTYVKKMKNFSKNLFI
                     WQVIQAVFAILSICLAILLTKIYLPGIVLFIVMATAGFFAGWEFPLINSLYLTDDKTL
                     TDSIGRFYTIDLIGATTGSLLTATVLVPIFGITITCTIFLAIKIIMAVAVMKVLLKIS
                     "
BASE COUNT         2473 a         1414 c         1543 g         2525 t
ORIGIN      
        1 ggtttgccat tgcaaagatg ataggatttg ctttcatctt tttaaccatt tctggactaa
       61 caagattggg acctgatagc ccaatgaaaa tatctgcatc ttcaagggca tcagcaagtg
      121 ttcctggtct ggatttcatc agataccgaa gtatctcttt ttttgcagag ttcatgccag
      181 tttttctttc cctgcatacg attccatgtc tatcgcacat tacgatattt cttatcccat
      241 atccatacag aatttttgta accgcaattc ctgcggctcc tgcaccatta atgacaaccc
      301 ttacagaacc tggctttttg ccagccagtt tcaaactgtt aatgattgca gcgagaacaa
      361 cgattgctgt gccatgctgg tcatcgtgaa aaacaggaat attaagtttt ttcttcaatt
      421 cccgttcaat aataaaacat cgtggactgg aaatgtcttc aagattgatg ccgccaaaac
      481 caggtgcaat tgcgcaaaca atctgaatga ttttttttgt attttgagta tttaggcata
      541 ctggataggc atccacaccg ccaaattctt tgaaaagtat cgccttgcct tccatcacag
      601 gaagagccgc aagtccgccg atatttccaa gtccaagaac agcgcttcca tcagtgacaa
      661 cagccactga attttttttg attgtgtatt gataagcaag ttcaggattt ttttctatga
      721 ttttacatac ctctgctacg ccaggtgtat aagcaattga aaggtctttt tttgttttca
      781 acgggacttt cattgagata ccaagttttc cgtcattatg gagtttcaga atttctttta
      841 aagtgaaatc agccatattt ttatcctttt ccgttaaata ctgccatcgc tttttcaatt
      901 ccttcattca aaaccaattc aattgcttct gctgcatctt ttatcatttg ttgagcaatt
      961 ggtttttcgt cttcatgaaa atcagaaagc acataatcaa caaggtccat gttttcagat
     1021 ggtattccaa tgccaagttt aatccttgga aatttttctg ttccgatggc ttcaatgaca
     1081 gattgaactc ctttgtgtcc accgctacta ccttttttcc ttattcttat ttttcccaac
     1141 gcaagattga tatcatcata tacaagaaga atttcatctg gtttaagacc aaaacctgtg
     1201 cacagggcaa gagcggcttt accgctttca ttcataaagg tggatggttt tgcgacaatc
     1261 acatttctac cgcatatttc tatttcacag gcacgaaaat actttctttt ccaacttttt
     1321 atcaacctgc tacgagaaat taattctgaa atgaccttga ctccgacatt gtgccgattg
     1381 ttgaggtaac tttttccggg gtttccaagg ccgagtataa ccacaattta ttcacccttt
     1441 tcagcttctt tttttgcttc tttttctttt ctgcgttcct cagcaatttc ctgcgtgatt
     1501 acttttggtt gttgaacttc ttctgtcgca actgcagcgg cttcttcagg cgcagggact
     1561 tcttctgctt ttggagcaag aattgtaacg acaacatgtt cagaatcaac aaggggtttt
     1621 ataccttcag gaaatgtcat atcccggaca tatatgctat caccaatttt aagattagag
     1681 acatctattt ccactgcttc tggtatggcg tcaggaagac cctcaacttc tatttctctg
     1741 aggtcatgtt ccatgatacc accctctttt actccagggc attcaccgat taatctgatt
     1801 ggaatactta aactaacggg ttgattctta cctatatgat aaaaatctat gtgcagcacc
     1861 tcgcctgtaa taggattgga ctgaacttcc ttcactattg ttttaagaat gttctttcct
     1921 tcattgctct ggatttcaag gtcagccatt acactgtgac ttgcaacatg tttgagaaca
     1981 atttctgcgg attttttgtc cactgcaaga agcttatttt cctcgcctgc tccataaaga
     2041 attcctggaa taaaaccatt tcttctaaga gcacgactct cactttttcc agcagagttt
     2101 ctcatttgtg caacaaattt aacctgtttt ttgtcttttt ctgtcatttt tttaccccct
     2161 gatgcctgat atcggctaat aataaagttt accattaaaa ggcaaattgt caatagaact
     2221 tttatgtggt aaaaaatatt attatggaaa tattttttag aaaagacctt cttgaataca
     2281 gggaaccaga acatcctgaa tgtcctgaaa gacttgaagc aattgcgaat tttcttgaga
     2341 aaaaagggaa gaaccaattt catccttttc agcagatacc agaatctttg cttctggagg
     2401 ttcactcaca gcgacttgta aatatggtga gaaataacac tttttttgat cctgataccc
     2461 caaacattca aaatatttac acatatgcat gtctatcagc cgggggggca gtggctgctg
     2521 cacagagcgc tcttgatggg aaagatgcct gtgctcttat tcgtccacca ggtcatcatg
     2581 caggtaaaga ttttcttggt ggattctgtt atttcaataa tgtggcaatt gccgtacaat
     2641 actgtatgaa aaaggtgaaa aaaattgcta tcctggacat tgatggacat catggaaacg
     2701 ggacagaggc agtattcaag aatgtcgata atgtaatgtt tatttccata caccagtatc
     2761 ctgcatatcc tggaactgga ctggcgagtt tttcaaattg ctataatttc cctgtgaaac
     2821 caaacactgc gttttctgat tacagaattt ttctggttga ggcgatgggt ttgataaaaa
     2881 gatttgctcc tgacattctg gggatatccc tcgggtttga cacccattta gatgatccgc
     2941 ttcttcagct gcgttttgtt gattccgact attatcatat ggcaaaaatg attgcttcag
     3001 gaatttcatg cccggttttt attgtttttg aaggcgggta tgatactgtt tctatcggaa
     3061 atagtttttt ctgttttctt tctggttggg aaaagaccaa aaatggcgct ggataacttt
     3121 tttgatttct cacaaaacag gatataatct tattcacatt cacaaaaggc agcacaatga
     3181 aaaatatcag agtggttttt gaaccaggaa tgatcgaggt tgatgtccct gaaggaagca
     3241 agattatttc tgcaatagaa cgttcgggaa ttccgtttga acttccatgc ggtggaaagg
     3301 gtatatgcgg aaagtgcggg gtaattgttc ttgaaggcgc atcaactcca acagaaacag
     3361 aaaagaaact tctcggcggc aaggtaaaat ctggatggcg acttgcctgt cagtcaagaa
     3421 tttatcatcg ttgtaaaatc ttgatcgaag gaaaaagttc ctcatatcac aagattctta
     3481 caacaaaggt tctgtgtgat atgccgagga atcctcaaat tacaaaaaag cacatcgtgc
     3541 ttccacaaat tacccttgat aacccagttt cattgcagga gcagatggca atgtttcttg
     3601 ggatgggtat tgagcaggtc tcacccttgt tactcagaga tccgtcattg tatgaaggaa
     3661 atgattttac actggttttt tgtaatggaa acattatatc aattgaaaaa ggcgatacga
     3721 cagatcaaat ttatggcatt ggttttgacc tgggtaccac cacaatggtg ctgactgttt
     3781 ttgaccttgt taattgcagg gagattatta ctgttgtcag accaaatccc cagataaaat
     3841 tcggagatga tgtaattagc aggatagatt ttagccttaa agaatcagga ttgaatactc
     3901 tctgcagggt tgtcgttgat gaaatcaaca aaatgattga tgaggcagta aaaacagcag
     3961 gtattaagag gcaacacata tatcaatttg tattgtcagg caatactgtg atggaacata
     4021 ttttcttgaa cctgcccctg aaaacattga gtagaattcc ttttaatcct gtcatgaaag
     4081 gtcctgttga atttcctgca tacacaagcg ggttgcatat gaatcctgaa ggtaaattgt
     4141 ttgttttccc tatacttgga ggatttgttg gaggcgatac gactggttta attcttgcga
     4201 caggcattca taaatcagaa aaaattcaag tgggtattga tattggaaca aacggagaaa
     4261 ttgttattgg taataaagac ggcattatcg cggcgtcaac tgctgcagga cctgcttttg
     4321 aaggaggcag gattacattc ggtatgcgag cacagatagg tgctcttgaa aaatgctggt
     4381 tttctgatgg aaaattacaa tggcagatta tcggaggaaa cagtttaaaa ggaatatgtg
     4441 gttcaggact tgtggatttg attactgtgt tgagacagca ggggattatt gatgaaacag
     4501 gcagatttga cgaaaattcg tcatcgcaac tggcagaact gctgaaatct cgggatggaa
     4561 atcagttatt caatcttgaa aatgataatc aatctattta tctcacacag aaggatgtca
     4621 gagaatttca ggcagcgaaa gcagcaatca gaactggcgt tgaaattttg atggcgataa
     4681 gaaatgtaaa aattgatgat attgaacacg tatatcttgc gggtgcgctt ggtaattttg
     4741 ttaacattga aaatataaaa aacttgaaga tgcttcctga tttccgtgca gaaacaattg
     4801 tcccttcagg taatacatct cttgcgtcca ctctgctttt tctatgtaat gctgatttaa
     4861 gaaaagacat tgagtacatt gcaggaataa caaaaactgt tgaattatct ctttatccat
     4921 cttttcagga tatttttaca gaagcattat tgttctgaat tatgaacaat cagcaacttg
     4981 tttccattgc gattgatatt ccagccagaa caaattttac ttataaaaca gacattttgt
     5041 tgaaaccagg gcaaagggtt cttgtccctt ttggaaaaag aaaaatgctt ggctgggtca
     5101 ttggtccagg ggtagaaggt ccctatcaat ataaaaaaat tttcaaggtg tatgacgaat
     5161 atccattgct tccacagcat cttttgaatc tcgcttttga aattgcacat atgtattttt
     5221 gttcagttgg ttcggtgctg gcgatgatga gtaagaattt gagtctaaaa aagatagata
     5281 cagaaattaa gtcagtatct ggtacaaaga cacctgattt taacaatgag atagcagaca
     5341 gaatttttga agatattaac aaaacaggga gaaaattttt tgtttgccaa ttcaacagaa
     5401 tggaagaaaa aattaaattt tttcaagcac ttccaacaaa aattcaggga tcatgtgcat
     5461 tcgtgatcag taattacttt gacgcaaaaa agcatgcgga tacattgaaa aatatctttg
     5521 gagacagggt catatttttt acaggggaat ccagtaaaac agaaaaagtg tttctctggc
     5581 acaggatgtt gaatgaaaaa aacctcatca ttatcgggac tcgtcttgca ctttttactc
     5641 ctgtttctga cctcgaaatt cttattgtgg atgaaccatc cgaatatggt cacaaagaaa
     5701 atcagacacc tcgttataca tcaagagaag ttgcaacgaa aatttctcaa ttgcttggta
     5761 ttcctgtggt ttttactgta tttcaaccag atgttacaga tatatttctc ctgaaaacca
     5821 aaaaagcagt tttgattgaa gcacagcaac gaaattttcc aagagttgta atcagtagaa
     5881 tgcagggtaa aaaacataca gatattctta cagatacggc aaaacatttt cttgaaaaaa
     5941 gtgtgcttga aaaaggaaag gttgttatca ttcataatct caaaggatat gcaagattgg
     6001 taatctgtaa aagttgtggc gcaagtgtac tgtgtcagga gtgtggaagt cctgtatgtg
     6061 ctgtgaacga gaattttact ttctgcacaa agtgtaaaaa aattttcccc atttcaaaga
     6121 aatgtttcgt atgtaaaaaa gcaacactca gtatgagacg tttaggaatt caaaaaatag
     6181 caaaaatgtt gaaaacaatg tatccggatt ttgacgtggg tgttcaggat gacggtgata
     6241 caatagatat gaatcggcaa atcattatag gcacacagca tcttgtttcc catgtacatg
     6301 acatttcacc atctcttttg atttttactg atgctgatat gattgcggca agaagcgctt
     6361 tcagaagtga agaaaagttt ttcctgcttg ttgaaaaaat aaaaagaatg atggagccgc
     6421 agcatatgat cattattcaa accagaaatc caggacttga actttacaca gatgttgtta
     6481 acaatagcca tgaaagtttc tacagccgtg agctggcgat aagaaacagt cttatgtttc
     6541 cgccttatgg agatttcatt gagattggcg tttctgggaa acattggaag aaaaacaaag
     6601 atgagatatt tgagaaactg aaaaaatctg gtgaaattta tgaggtgtct gaaaagatga
     6661 aaaaaacagt atttttatgg aaaattacag aaagaaataa tgcttttgaa attctcaaga
     6721 atgtgcttga aaaatacagg atacacaatt attccattga tataaatcca tatttttaag
     6781 aaattttcag caatactttc attacagcaa cagccataat aattttgatt gccagaaata
     6841 ttgtgcaggt tattgtaata ccaaaaattg ggacaaggac tgttgcagta agtaaactgc
     6901 ctgtggttgc accgataagg tctatggtat agaacctgcc tatggaatcc gtaagagttt
     6961 tatcatctgt cagataaaga ctgttaatta aaggaaattc ccatccagca aaaaatcccg
     7021 cagtagccat cacaatgaaa agaacaattc caggtagata tatttttgtg agtaaaatgg
     7081 caaggcaaat acttaaaata gcaaaaactg cctgtatgac ctgccatatg aaaagatttt
     7141 ttgaaaaatt tttcattttt tttacgtaag ttggaaaaat tatacttccc gcagccatac
     7201 cacccataaa aactcctgtg agtatgctta aataaaaata tactgaacca aaattaatct
     7261 ggaacagaaa aaggtaaatg agttcccatg caattgatag caaacctgtt gaaaacattg
     7321 tcaattcaag tatcctggtt tttgttggtg tttttacaac atatataatc gtgcagacaa
     7381 gaaaaatggt tagtaataca atgcttatgg tgacataatg attcatatca caggattttt
     7441 tgccactttc tgacattgaa atccagtact taatagcggg tattataata atgggtttca
     7501 attgagtgtt aatcaatatc ttgtcagtgc ttatcatttc aaggaaatga tgtctttttc
     7561 ctgtatctaa cctatcagaa acataaaaat cagtaaccaa acctgtttga atttgtttct
     7621 tttttgactg ggaaataatg aaggaagacg aaaattcagg catgttagag ttcaccgcag
     7681 cgcaataacc ggtgaaatat ccaggaatta cgtgaaccga tggaaaaaca tttttcaaag
     7741 ttgcgtacgt gatgttgtga acgtctgcaa gtggttcgga catatattca ggagaacccg
     7801 gaagcaaggt ataaaaaatt ccatcctgcg aaagatgggt ttttacaagg ttgtagaatt
     7861 ctttcgtata aaaatttgcg atttttaatg agaaaggaag ccctgcgtca tgaataatga
     7921 cgtcccagtg tttttttgtt tttttgaaaa aactg
//
LOCUS       DAORYU010000042         7926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_6540_length_7926_cov_3.726972, whole genome shotgun sequence.
ACCESSION   DAORYU010000042 DAORYU010000000
VERSION     DAORYU010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7926
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6540_length_7926_cov_3.726972"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..474
                     /gene="rplP"
                     /locus_tag="PLW07_02280"
     CDS             58..474
                     /gene="rplP"
                     /locus_tag="PLW07_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOL49381.1"
                     /translation="MPLMPKRVKYRKVQRGRTKGKATRGATIYFGDFGLQVLSPGRLK
                     SNQIEAARIAITHHLKAKGKLWIRIFPDRPFTKKPAETRMGKGKGDVAGWEAIVLPGR
                     IVFEIAGVSADVAMEALRLASYKLPIETKIVKREIQ"
     gene            471..707
                     /gene="rpmC"
                     /locus_tag="PLW07_02285"
     CDS             471..707
                     /gene="rpmC"
                     /locus_tag="PLW07_02285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOL49382.1"
                     /translation="MKRQDFEELKSLTVQELEQKRTDIARQLFDQRIQVQLGQLKNTR
                     ILRNLRRDIARINTIIQLKKISKQRGANGSETRK"
     gene            685..990
                     /gene="rpsQ"
                     /locus_tag="PLW07_02290"
     CDS             685..990
                     /gene="rpsQ"
                     /locus_tag="PLW07_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006747231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOL49383.1"
                     /translation="MVPKQENKIEKQGQVVSDKMEKTRVVVVETLSRHPVYFKVIRKR
                     KKFYVHDESEISKTGDTVIIRQCRPLSRLKRWRIVEVVKKASSKSEGVTNGAVKNNT"
     gene            965..1333
                     /gene="rplN"
                     /locus_tag="PLW07_02295"
     CDS             965..1333
                     /gene="rplN"
                     /locus_tag="PLW07_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOL49384.1"
                     /translation="MVQLRTILEVADNTGAKKISVIGVLGHSKRRYARIGDIIRANVK
                     DALPDGIVKKKQVVKAVIVRTKAPIRRADGSYLKFDHNCAVIIDDDLNPRGTRVFGPV
                     PRELREAKFMKIVSLAPEVL"
     gene            1335..1658
                     /gene="rplX"
                     /locus_tag="PLW07_02300"
     CDS             1335..1658
                     /gene="rplX"
                     /locus_tag="PLW07_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOL49385.1"
                     /translation="MGFLIRKNDFVVVIKGKDTGKKGRVVRVFPETSRVLVEGVNFVK
                     KATRPRKLDRQGGIITMEKPISIANVQFFCMKCSKKTRLGIKMMDDGSKVRYCKKCGE
                     LVEAK"
     gene            1658..2203
                     /gene="rplE"
                     /locus_tag="PLW07_02305"
     CDS             1658..2203
                     /gene="rplE"
                     /locus_tag="PLW07_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010469306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOL49386.1"
                     /translation="MEARLKILYREKVVPSMMEKFGFKNSHQVPCIQKVVINVGVGKD
                     NKDAKAIESVQKELALISGQKPVITRGKKSISSFNLRKGDICGVMVTLRGQRMYEFLD
                     RMITAALPRVKDFNGLNPDSFDDRGSYTLGIREQVIFPEVDYNTIYKVRGMNITICTN
                     AKKVEHARELLKLMGLPLRED"
     gene            2205..2390
                     /locus_tag="PLW07_02310"
     CDS             2205..2390
                     /locus_tag="PLW07_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOL49387.1"
                     /translation="MARKCFFDKMKKEPKFEVRKRNRCWRCGRPRGYLRKFGICRICF
                     RQLASQGEIPGVTKASW"
     gene            2402..2791
                     /gene="rpsH"
                     /locus_tag="PLW07_02315"
     CDS             2402..2791
                     /gene="rpsH"
                     /locus_tag="PLW07_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOL49388.1"
                     /translation="MTDPIADMMTRLRNANIVFKPYADVSWSKLKEGILGVLKEEHYI
                     KDWEIIEADGKKILRVHLLYSEKKPTISKVRKLSKPGCRVYISADKIAKEKHDTGIAV
                     LTTSKGIISSRKAKELNVGGELLFYIW"
     gene            2801..3373
                     /gene="rplF"
                     /locus_tag="PLW07_02320"
     CDS             2801..3373
                     /gene="rplF"
                     /locus_tag="PLW07_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007391733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOL49389.1"
                     /translation="MGRLGKKPVEIPDGVKITMEGRVLSIEGPKGRITQNVFENLDVT
                     VEDKKIFVVNTVPQTTKFRRLFKKTDALQGLLRTLIKNRILGVTKGFEKTLEIHGTGF
                     KAEVKGKKLMLNLGFTHPVEVDIPEPITVQIEKNTVMKISSYDKDLLGNFAADIRDIY
                     PPEPYKGKEIRYAGEYVRHKVGKAAVGTQK"
     gene            3389..3763
                     /gene="rplR"
                     /locus_tag="PLW07_02325"
     CDS             3389..3763
                     /gene="rplR"
                     /locus_tag="PLW07_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007132555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOL49390.1"
                     /translation="MLSRQDKRKKRHLRVRKQISGNLQRPRLVVCRSSKHIYGSLVID
                     DVQPNKVLLTVSSLGSDFKKVAKPEEKGGNVKGAFLVGKLLAERAKSMNIETVVFDRA
                     GYGYKGRVKSFAEGARQGGLKF"
     gene            3823..4254
                     /gene="rpsE"
                     /locus_tag="PLW07_02330"
     CDS             3823..4254
                     /gene="rpsE"
                     /locus_tag="PLW07_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005868986.1"
                     /note="located at the back of the 30S subunit body where
                     it stabilizes the conformation of the head with respect to
                     the body; contacts S4 and S8; with S4 and S12 plays a role
                     in translational accuracy; mutations in this gene result
                     in spectinomycin resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOL49391.1"
                     /translation="MEIRRVMKVTKGGKRLKFRAVIVCGNQKGEAGFGVGKASEVPEA
                     IRKGREKANRNRIAISLNGSTIGAAIEAKYDASHVILKPASKGHGLVAGKTMRAFLEV
                     LGVRDAVCKVIGSTNTINVLNATYRALKFLELKEKKSVENP"
     gene            4241..4690
                     /gene="rplO"
                     /locus_tag="PLW07_02335"
     CDS             4241..4690
                     /gene="rplO"
                     /locus_tag="PLW07_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012991790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOL49392.1"
                     /translation="MKIHDLRPTKGSKHKKKIVGRGHGSGHGQQSTRGHKGHKSRSGF
                     HRVGGFEGGQMPLIRRIPKRGFNPLSKTIFEEVNIGQVNSKFNAGDVVSPDALAEKGL
                     VKNKKNLVKILGEGKIEKSLEIHAHAFSEKAKNMIEKAGGKIMVLKK"
     gene            4687..5997
                     /gene="secY"
                     /locus_tag="PLW07_02340"
     CDS             4687..5997
                     /gene="secY"
                     /locus_tag="PLW07_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006419459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOL49393.1"
                     /translation="MITAFKDTLKVPDLKRKILIVVALLTVYRFGCYIPVPGINGKAL
                     ASFFDRMSGTLFGIADLFAGGALSNATIFALGIMPYISVSIILELLASIFPFFEHMLR
                     SGVEGRRKLTQWTRYGTVILCLFQGFAVSVWLENPAHFDGVVMVNSPGWLFRITTVLT
                     LTTGTIFLMWLGEQITEKGIGNGISLIITASILSRMPVAVHQTAQLMSAGEINPGLIV
                     LMAGLIFVVVGAAIMLNEGERRIPVQYAKRVVGRRVYGGQTTYLPIKLNPGGVLPLIF
                     AVSFLTFPSTILRFANNPFLQKLAHVLSPVSGPGMVLYALLIVGFCYFYASVIFNPEH
                     VSDDLKKYGGFVAGIRPGKATGEYLERILGRLTLPGSLMLTVIAIFPYIIMHIFRIPY
                     LIASMFGGIGLLIVVSVILETLRQIEAHLIMRHYEGFLKKARAR"
     gene            5994..6641
                     /locus_tag="PLW07_02345"
     CDS             5994..6641
                     /locus_tag="PLW07_02345"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HOL49394.1"
                     /translation="MRKIIILFGPPGIGKGTVGKSLSELLGFPIISIGDLLRENVKKS
                     TSIGVKAEAFMKKGELVPDDIVFEALNDRIQQEDAINGFLLDGFPRNMNQARMFEKTI
                     DENDRVVVLNFVAPDSVLIERLSLRRICRNCGAIYHLKHMPPKKEGICDICGGPLIQR
                     EDDTPQVIARRLDVFRSETEPLLEYYGGKHRVLTVSAADNRENILRRIEGLTLWV"
     gene            6632..7387
                     /gene="map"
                     /locus_tag="PLW07_02350"
     CDS             6632..7387
                     /gene="map"
                     /locus_tag="PLW07_02350"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOL49395.1"
                     /translation="MGVKRPDEIEKIRSAGKICKKILLEISNVLEEGKTTLDIADFAE
                     LLMEKEKVKSAFRGYRGYPGVICISINEEVVHGIPSKSRYLRYGDIVSLDVGVVKDGY
                     IGDCAITCPVGEIDPEKQRLIDVTRKSLELGISKVKAGCRTGDIGSEIQRFVEKEGFN
                     VVRDFAGHGVGKYLHEEPDVPNFGRRGKGFELQENMVIAIEPMVNMGKPDLRILSDGW
                     TVVTRDGLPSAHFEDTVLVKKEGFEILTNFHNG"
     gene            7380..7598
                     /gene="infA"
                     /locus_tag="PLW07_02355"
     CDS             7380..7598
                     /gene="infA"
                     /locus_tag="PLW07_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015164369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOL49396.1"
                     /translation="MDKKIDKITLTGTISKALPGTSFLVDLENGMKINAHLCGKMRLH
                     YIKIIPGDKVIVEITPYDITKGRIIKRL"
     gene            7608..7733
                     /gene="rpmJ"
                     /locus_tag="PLW07_02360"
     CDS             7608..7733
                     /gene="rpmJ"
                     /locus_tag="PLW07_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOL49397.1"
                     /translation="MKVRGSVKKMCPQCKIIKRRGSVMVICKNPQDGPKHKQKQG"
     gene            7746..>7926
                     /gene="rpsM"
                     /locus_tag="PLW07_02365"
     CDS             7746..>7926
                     /gene="rpsM"
                     /locus_tag="PLW07_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004101491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOL49398.1"
                     /translation="MARILGVEIPNEKKVLIGLTYIYGIGKSIAERIIKATGVNPDKR
                     IKDLTEEEIGKISAFV"
BASE COUNT         2632 a         1188 c         1965 g         2141 t
ORIGIN      
        1 cattgaatat tgaaatatct catcctgtag aggaacttct tagtaaaagg agcaaaaatg
       61 ccgttaatgc caaaaagggt aaagtacaga aaagttcaga gaggcagaac aaagggtaag
      121 gcaacccggg gagcaacaat ttactttggt gattttggac tgcaggttct atcgccgggg
      181 cgactcaaaa gtaatcaaat agaagcagcc cgtattgcta ttacccacca tttgaaggca
      241 aagggtaaac tctggatacg gatatttccg gacagaccct ttaccaaaaa acctgctgag
      301 acgagaatgg gtaaaggaaa aggagatgtg gccgggtggg aagcaattgt acttccaggt
      361 cgaattgttt ttgaaatagc cggtgtatca gcggatgttg ccatggaagc gttgagattg
      421 gcttcttata agttaccgat tgaaacaaaa attgtgaaac gggaaattca atgaaaagac
      481 aggattttga agaacttaaa agtttgactg ttcaggaact tgaacaaaaa aggacggaca
      541 ttgcaagaca gctttttgat cagagaatac aggttcaact ggggcagttg aagaatacgc
      601 gaattttgag aaatttgaga agggatatcg cccgcataaa taccataata cagttgaaga
      661 aaatcagcaa gcaacgaggt gccaatggtt ccgaaacaag aaaataaaat tgagaagcag
      721 gggcaggttg taagtgataa gatggaaaaa accagggtgg ttgttgtcga aaccttgagc
      781 aggcatcctg tgtatttcaa agtgattcga aaaagaaaga aattctacgt tcatgatgaa
      841 tctgaaatta gtaagaccgg agatacagtc attatcaggc aatgcaggcc attaagcaga
      901 ttgaaaagat ggcgaatagt agaggttgta aaaaaagcat catccaaaag tgaaggagta
      961 acaaatggtg cagttaagaa caatacttga agttgcggac aacacaggtg ccaagaaaat
     1021 aagtgttatc ggggtgcttg gacactcaaa gcgaagatac gccagaatcg gggacatcat
     1081 aagagcaaat gtgaaagatg ctttgcccga tggaatagtg aaaaaaaagc aggttgtcaa
     1141 ggccgtgatt gtgagaacaa aagctccgat tagaagagca gatggttcat atttgaagtt
     1201 tgatcacaac tgtgcggtta ttatagacga tgatcttaat cccagaggca caagagtttt
     1261 tggtcctgta ccaagagaac tgagagaggc aaaatttatg aaaatcgttt cactagctcc
     1321 ggaggtacta taagatgggg tttcttatca ggaaaaacga ctttgtagtc gttattaagg
     1381 gtaaagatac aggtaaaaag ggcagggttg tcagggtttt tcctgaaacg agtcgcgttc
     1441 tggtggaagg agtgaacttt gtaaaaaagg cgacaagacc caggaaactt gacagacagg
     1501 gtggaatcat aacaatggaa aaaccaattt caatcgccaa tgttcaattt ttttgcatga
     1561 agtgtagcaa aaaaacacgg ctgggaataa agatgatgga tgatggttca aaggtcagat
     1621 actgtaaaaa gtgcggcgaa ctggtggagg caaaataatg gaagccaggt tgaaaatact
     1681 ttacagagaa aaggtggtac cttcaatgat ggaaaagttt ggatttaaga actcgcacca
     1741 ggtcccttgc attcagaagg ttgttatcaa tgttggtgtt ggtaaagata ataaggatgc
     1801 aaaggctatt gaatcagttc agaaggaact ggctttaata tcaggacaaa aacctgtcat
     1861 tacaaggggt aaaaagtcaa tttcttcttt taatctgaga aaaggtgata tttgtggtgt
     1921 gatggtaaca ttgagaggtc agaggatgta tgaattcctt gatcgtatga ttacggctgc
     1981 gcttccaagg gtaaaggatt ttaatggatt aaatcctgat tcctttgatg accgtggcag
     2041 ctacacattg ggcatcaggg agcaggtgat tttccctgag gttgattaca ataccatcta
     2101 caaggtcaga ggtatgaata ttacaatatg tacgaatgct aaaaaagttg aacatgcaag
     2161 agaactatta aaacttatgg gattaccatt aagggaggat tgatttggct cgtaaatgtt
     2221 tttttgataa gatgaaaaaa gaaccgaaat ttgaagtaag aaaaagaaat cgctgctgga
     2281 gatgtggtag accaagagga tatttgagaa aatttggaat ttgccgtatt tgtttcagac
     2341 aactggcatc acagggtgaa atacctggtg tcaccaaagc aagctggtag gaggaataat
     2401 tatgacagac cccattgctg atatgatgac gaggttgagg aatgcaaata ttgttttcaa
     2461 gccatatgcg gatgtatcgt ggtcaaaact taaagaaggc attcttggtg tgctgaaaga
     2521 agaacattat attaaggatt gggaaattat cgaagccgat ggtaaaaaaa ttctcagagt
     2581 acacctgttg tattcagaaa aaaaaccaac gatatcaaag gtgcgtaaat taagcaaacc
     2641 aggatgcagg gtgtacattt cggctgataa aattgcaaaa gaaaaacatg ataccgggat
     2701 cgcggtattg actacgtcta aaggaatcat aagtagccgt aaggcgaaag aactgaacgt
     2761 tggtggagaa cttttatttt acatatggta ggtgaatact atgggaagat tggggaaaaa
     2821 acctgttgaa atacctgatg gtgtaaagat aacgatggaa ggtagggttc tgtctattga
     2881 aggtccaaag ggcaggatta ctcaaaatgt ttttgagaat ctggatgtaa ctgttgaaga
     2941 caaaaagatt tttgtagtta atactgtgcc acagaccaca aaattcagaa ggttgtttaa
     3001 gaagacggat gcccttcagg gtttgttacg aacattgata aaaaatagaa tcctgggcgt
     3061 tacaaaaggt tttgaaaaga cacttgaaat ccatgggact ggattcaaag cagaagtaaa
     3121 agggaagaaa ttgatgttga accttggatt tacacatcca gttgaggtgg atataccaga
     3181 accaattact gtgcagattg agaaaaacac agtgatgaag atcagttcat atgataaaga
     3241 tcttctggga aattttgccg ctgatatcag agatatatat ccgccggagc cgtacaaagg
     3301 caaagaaatc agatacgcgg gtgaatatgt aagacataag gttggtaaag ccgctgttgg
     3361 tactcagaaa taagggagtg aacatatgat gttaagcagg caagataaaa gaaaaaaaag
     3421 gcatttgcgt gtaaggaagc aaatttcagg gaatcttcaa cgaccgaggt tagttgtttg
     3481 cagaagttca aaacatattt atggttctct ggttattgac gatgttcagc caaacaaggt
     3541 acttttaact gtttcttctc ttggcagtga tttcaagaag gttgcaaagc cagaagaaaa
     3601 aggtggaaac gttaagggtg cttttcttgt tggcaaactt ctggcagaac gtgcgaaatc
     3661 tatgaacata gaaactgtcg tttttgacag ggccggttat ggctacaaag gtcgtgtaaa
     3721 atcttttgcc gagggcgcaa gacaaggagg tttaaaattt tagcaagtga aaatcttatt
     3781 ccgcagattg aacaggaaac tgcagaagag aaggaaattg ttattgaaat ccgcagggtt
     3841 atgaaagtga caaagggcgg taaacgattg aaatttcgcg cggttattgt ctgtgggaat
     3901 cagaaaggag aggcaggatt tggagtgggg aaggcatctg aagttcctga ggcgattcgc
     3961 aagggcaggg agaaagccaa caggaacagg attgctattt ctttgaatgg ttctaccata
     4021 ggtgccgcta ttgaagccaa atacgatgcc tcgcatgtta ttcttaagcc ggcatcaaag
     4081 ggacatggac ttgttgccgg gaaaacgatg cgcgcttttc ttgaagttct gggtgtgaga
     4141 gatgccgtat gtaaggtaat tggttcgaca aataccatca atgttctgaa tgcgacttac
     4201 agggcactga aatttcttga actaaaggag aaaaaaagtg ttgaaaatcc atgatttaag
     4261 accgacaaag ggaagcaaac acaagaaaaa aattgttggg agaggtcatg gctccggaca
     4321 tgggcaacag tccacaagag gacacaaggg gcataaatca aggtcaggtt ttcatcgcgt
     4381 cggtggtttt gaaggcggac agatgccttt gatcagacgt attccgaaaa gaggtttcaa
     4441 tcctttgtca aaaacaatct ttgaagaagt gaatatcggg caagttaata gtaaatttaa
     4501 tgcaggcgat gtcgtatcgc ctgatgcact tgccgaaaaa gggcttgtta aaaacaaaaa
     4561 aaacctcgtg aaaatactcg gggaaggcaa gattgaaaaa tctcttgaga ttcatgctca
     4621 tgctttttca gaaaaagcga aaaatatgat agaaaaagct ggcggaaaaa tcatggtgct
     4681 caaaaaatga ttacagcatt taaagacaca ttaaaagtcc ctgatctaaa acgaaaaata
     4741 cttattgtgg tggctctgct gaccgtttac aggttcggtt gttatattcc tgttcctgga
     4801 atcaatggca aagcccttgc ttcttttttc gacagaatgt cagggacgct ttttggtatt
     4861 gctgatttgt ttgcaggtgg tgcattgagc aatgcgacaa tttttgctct ggggataatg
     4921 ccttacatca gtgtttcaat tattcttgaa ctgctggcgt caatatttcc attttttgaa
     4981 catatgttga gaagcggagt ggaaggtaga agaaaactta cacagtggac acgttatggc
     5041 acggttattc tgtgcttatt tcagggattt gcggtaagtg tatggcttga aaatcctgcg
     5101 cattttgatg gtgtggtcat ggtcaattct cctgggtggt tgttcaggat cacaacggtt
     5161 ttgaccctta caaccggtac catttttctt atgtggctcg gtgaacagat tacagagaaa
     5221 ggaattggca atggaatttc tcttatcatt accgcaagta tcctttcaag aatgcctgtt
     5281 gctgttcatc agactgccca gctcatgagc gcaggagaaa taaacccagg acttattgta
     5341 ttgatggcag gactgatttt cgttgttgtt ggtgcagcaa taatgttgaa tgaaggcgaa
     5401 agaaggattc ctgttcagta cgcaaaaagg gttgttggca gacgggttta cggcggacag
     5461 acaacttatt tgccaataaa acttaatcca gggggagtcc ttcctttgat ttttgctgta
     5521 tcatttctca cgtttccttc aacaatactc agatttgcaa acaatccatt tttgcagaaa
     5581 cttgcccatg ttctcagtcc tgtttcaggt cctggaatgg ttttgtacgc tctgttgatt
     5641 gttggatttt gctatttcta tgcaagcgta attttcaatc ctgaacatgt atcggatgat
     5701 ttgaaaaagt atgggggttt tgttgcgggc atcaggcccg ggaaagcaac aggtgaatat
     5761 ctggaaagga tccttggcag attaaccctg ccaggttctc tcatgttgac ggtgattgca
     5821 atatttccat acattattat gcatatattc aggattccat atctgatagc gagtatgttt
     5881 ggtggtatag gacttctcat agtggtttct gtaattcttg aaacactaag acagattgaa
     5941 gctcatctta ttatgcgtca ttatgaagga tttcttaaaa aggcgcgagc aagatgagaa
     6001 aaattatcat tttgtttggt ccccctggta taggaaaggg gacagtgggt aagagtctca
     6061 gtgaattatt gggatttcct attatatcaa ttggagattt acttagagaa aatgtaaaaa
     6121 aaagtaccag tataggcgtg aaggcagaag catttatgaa aaagggagaa cttgttccag
     6181 atgatattgt atttgaagcg ctcaatgaca gaattcagca ggaagatgca ataaatggat
     6241 ttttgctgga cgggtttccg agaaacatga atcaggcccg gatgtttgaa aaaacaattg
     6301 atgaaaatga tagagtagtt gtattaaatt tcgttgcacc tgacagtgtt ttgatagaac
     6361 gattgtcgtt aagaagaata tgcagaaact gtggagctat ttatcatcta aaacatatgc
     6421 ctcccaaaaa agaagggata tgcgatatct gcggaggccc tttgattcag agagaagatg
     6481 atacaccaca ggttatcgca aggcgtcttg atgtttttcg cagtgaaacg gaaccattac
     6541 ttgaatatta cggtggcaaa catagagtac taactgtttc tgctgctgat aaccgcgaaa
     6601 atatattaag aagaattgag gggcttacgc tgtgggtgta aaaagacccg atgagataga
     6661 aaaaattaga agtgctggaa aaatctgcaa gaaaattttg cttgaaattt caaatgttct
     6721 tgaagaaggg aaaacgactt tagatattgc agattttgct gaattgttga tggaaaaaga
     6781 aaaagttaaa tcagcttttc gcggatacag ggggtatcca ggagtgattt gcatatcaat
     6841 aaatgaagaa gtggtacatg gaataccttc aaaatcaaga tacctgaggt atggagatat
     6901 tgtgtctctt gatgtgggtg ttgtaaaaga cggttatatt ggtgattgtg ccataacctg
     6961 tcctgttgga gagattgatc ctgaaaagca gaggttgatt gatgtaacga gaaaatctct
     7021 tgaacttggg atatcaaagg tgaaagcagg atgcaggaca ggagatatag gaagtgaaat
     7081 tcaacgtttt gttgaaaaag aaggattcaa tgttgtgcgt gattttgcag ggcatggtgt
     7141 tggaaaatat ttgcatgaag aaccagatgt tcctaatttc ggacgccgtg gcaaaggttt
     7201 tgaattacag gaaaacatgg tgattgctat agaaccgatg gtcaatatgg gtaaacctga
     7261 tttaagaatt ctgtctgatg gctggaccgt tgtgactcgt gacggactac cctctgctca
     7321 ctttgaggat acagtgcttg tgaaaaaaga gggttttgaa attttaacaa atttccataa
     7381 tggataagaa aatagataaa attacactta ccggtacgat tagtaaagca ctaccgggta
     7441 caagttttct tgtggatctt gaaaatggga tgaagattaa cgcgcatctt tgtggaaaga
     7501 tgcggttaca ctatatcaaa attattccag gtgacaaggt aattgttgaa attacacctt
     7561 acgatataac caaaggtaga attattaaaa gattatagga ggaaaatatg aaggtacgtg
     7621 ggtcagtaaa aaaaatgtgt ccacagtgta aaattatcaa aagacgaggg tctgtcatgg
     7681 tgatatgtaa aaatccccag gatgggccaa aacataaaca aaaacagggt tgaggaggtt
     7741 taacaatggc tcgtattctt ggagttgaaa ttcctaacga aaaaaaggtg ctaattggat
     7801 tgacctatat ctatggtatt ggtaagtcaa ttgcagaaag aattatcaaa gccactggag
     7861 tcaatcctga taaaaggatt aaagatttaa cagaggaaga aattggaaag atttcagcgt
     7921 ttgttc
//
LOCUS       DAORYU010000043         7799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_6675_length_7799_cov_4.104468, whole genome shotgun sequence.
ACCESSION   DAORYU010000043 DAORYU010000000
VERSION     DAORYU010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7799
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6675_length_7799_cov_4.104468"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1312
                     /locus_tag="PLW07_02370"
     CDS             <1..1312
                     /locus_tag="PLW07_02370"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOL49399.1"
                     /translation="IGIMLFFINAKLAFYVLVPVPLVMMGTYFFWSRIYPMNFKLWDS
                     QAKVSRFLNIVLSGIKVVKAFAQEEKETKRFNTLASHLRDSQRRVSISVAKFNPMMAF
                     LFSTGGLIVWYAGGKAVLQNQGFTLGQLMAFLSYTGMFYAPLSQMALMSNWVSSFVTA
                     SHRIFEILDTEPQLKDAPGAIRMPHIRGHIRFEHVTFGYDPYQPIIKDVSFEIEPGQL
                     VGIVGKSGSGKTTLVNLVCRFYDVQEGAIYIDGKDIREISSYDLRKQIGLVLQEPFLF
                     RASIAENILYGRPDATFKDILNASKAANCHDFIMKLPMGYDLYLREHGAGISGGEKQR
                     IGIARALLCDPPILILDEATSSVDTESEKKIQDALAILCKNRTTIAVAHRLSTLRGAD
                     KILVVDNGKIVESGTHQQLMDKKGVYYKLVIMQTKLTSIETEAE"
     gene            1314..1841
                     /locus_tag="PLW07_02375"
     CDS             1314..1841
                     /locus_tag="PLW07_02375"
                     /inference="COORDINATES: protein motif:HMM:NF020483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1854 domain-containing protein"
                     /protein_id="HOL49400.1"
                     /translation="MGEDIKKPEIVFLDPKKIKIFRGTFETIHLMLEDGSLYRGVFAV
                     AAFPVSNPNKYISLFCYDERDREYEIGMIEDIEPLPEEAKKLIVDALKRQYFSFEILA
                     INSIKFAYGLLFFDVETDKGPREFSMKWETRFAFDFGRKGKILVDIFEDRYVIRDISK
                     LNKIEQELFTRYIYW"
     gene            1843..2400
                     /locus_tag="PLW07_02380"
     CDS             1843..2400
                     /locus_tag="PLW07_02380"
                     /inference="COORDINATES: protein motif:HMM:NF022751.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HOL49401.1"
                     /translation="MFFIGKILVSSEIVTCRFSCDINECKGMCCCEGEAGAPLEYSEI
                     EILTKNIPVIKNYLKKSSVDKINRDGCFVKNLWGGFETPIADNGWCIYAVEENGIVLC
                     GIEMAWKNKQIDFRKPVSCHLYPIRIRKFGIFEGLIYERWDVCRCKTLQESPFLIEFA
                     SEALERKYGNTFVEKLREISMKENT"
     gene            2397..3170
                     /locus_tag="PLW07_02385"
     CDS             2397..3170
                     /locus_tag="PLW07_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger Ran-binding domain-containing
                     protein"
                     /protein_id="HOL49402.1"
                     /translation="MTEVAPVLFILTITLVAVEAWWIWFMYPHDWYLNSGGFAFLEIL
                     TTGCLVLSFFSFAGKFPRNFIFFAPVIPAVACFTVRNLYKLFEDRYEGKKMKTEIDML
                     IRNAGKFGNGYTFEQIGDIYFSRRDFENALSWYLKASSAQESTEISHKINITRQEILL
                     KKKKLWICPECSMTNSTNSGSCKSCGALRPSIKTLKHEVQRTTPYLRKYLFVFAWLFL
                     FISFFTWFIKNANFFTSFIVFFVLFVILALYFLYRLFSN"
     gene            3184..4266
                     /locus_tag="PLW07_02390"
     CDS             3184..4266
                     /locus_tag="PLW07_02390"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOL49403.1"
                     /translation="MNSKTRVMNTVLRTNIDKIPLDIWATDETIKALCNHCSVSYEKL
                     LEKLDIDIRYIDLQIENENFKKMYDGSFQRQIDTHYFVDIWGVIRKKISYGKGEYFEL
                     DKSPLAEITDAKDVDLFQLPDPGDFDIATDHLELYDRFAIVFTGDRLTTRTSFFKLAM
                     YLRGFENFLLDLHINKQIAGAIIDKLFEFHYTLNESIFKRYADKIDVLLLGDDFGTQT
                     GPLIGLESFREFFKRPLREMIELCHKYNIRTMLHSCGSVKIFIPDLIEIGLDILNPVQ
                     HTAFEMDLRDLKREFGKDICFHGAIDVQKVLPFGSKKDIENEVKTCIEILGANGGYIC
                     APSHNLQSDVPVENIIYMYEYARSIR"
     gene            complement(4288..4425)
                     /locus_tag="PLW07_02395"
     CDS             complement(4288..4425)
                     /locus_tag="PLW07_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49404.1"
                     /translation="MCAIITLYHHHGNVFKLYWYSPHENNRIDSRTSQLPTPYSMVVP
                     K"
     gene            4542..5450
                     /locus_tag="PLW07_02400"
     CDS             4542..5450
                     /locus_tag="PLW07_02400"
                     /inference="COORDINATES: protein motif:HMM:NF021982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HOL49405.1"
                     /translation="MKISCCILSGLFMILSVVVAGQTIEQRTINVDGIKRIYRIQIPA
                     CYNFDRSFPLVFVLHGGGGSGETISNFTGFKMLAEKNRFIVVYPDGYQKQWNDGRDYS
                     VTGVADVKFLRAVFEIVSQEFNIDKTRVYAAGISNGAMMCYRLGIEMSDIFAAIAAVC
                     GNLPTKLEEKKPEHPVSVLIMNGTSDPLVPYEGGQVKVFGKNRGSVISTERTVEFWIK
                     ANKCFEKLPDKVIDVDKNDGSFISVYSYVNNRTQVVLYKIIGGGHTWPGGVQHLPEKI
                     IGKTNRDIKAEHVIWDFFSRCKKETQ"
     gene            5447..6553
                     /locus_tag="PLW07_02405"
     CDS             5447..6553
                     /locus_tag="PLW07_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOL49406.1"
                     /translation="MTTSARKLRDIYGRKPYAPFFQKEFGFFTLERWKKEGKIDDNTN
                     LDELFGFDPPGNYNLAQLGWTIAPFEPAFETKIIEDKIDYELVQDESGRHVLFFKGRR
                     NGFMPEYVAHPVKDWKTWIENVKWRLDLRTETRRKHFLKVIPELIDAQNKGLMITERV
                     IGGYMYLRCLIGPSELPLFFYDHPDVIHDCMKTWFELANAIIEMHQQYTVIDELFFGE
                     DICYNHGPLISPQMIKEFLFPYYQQLITNIKSRQAKSGRHLYFHLDTDGYAVSVIPLY
                     KELGMDVMSPFEVASGCDVVRDGQTFPDIAMFGGIDKRVLAKTKDEIDRHLDRILPAM
                     YKRGGYIPTCDHGVPEEVSFENYMHYRKRILEYN"
     gene            6559..7128
                     /locus_tag="PLW07_02410"
     CDS             6559..7128
                     /locus_tag="PLW07_02410"
                     /inference="COORDINATES: protein motif:HMM:NF012783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOL49407.1"
                     /translation="MNRKNVVIKFFAPVMDASINALMNAIDQKMREGISKFTILISSP
                     GGSVFHGLSAYNYLKGIPAEIITHNFGSVDSIGVVLFCAGEKRLSVPQARFLLHGVSV
                     AFRQNENLEEKQLEERLKGLRIDIENIAKIISSNSNKGINQVISAMLERTTLNPQQAI
                     EWGLVHEIVPELYESCSDVISIHFQSHQT"
     gene            complement(7132..>7799)
                     /locus_tag="PLW07_02415"
     CDS             complement(7132..>7799)
                     /locus_tag="PLW07_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49408.1"
                     /translation="EWRQYKRKITAREWDYDFRRIFFSFTPDITKEPFRYWSEISNRE
                     KTAGNARNQDLYADNDDIIHLLWMEKSCDERLREQFFPDEILTYSLEYAQLKKQKVLE
                     RKTILLFSEQYPEQYPCGMKNINFFWGRFHITRKGKLLVFASLHGKDENNNTVAENYL
                     ISLDSTGNIVDKKIVNFEKPFTLFHTASIRNGSFPSDIIHIYGHITNPDNEMWYGRIE
                     IKD"
BASE COUNT         2461 a         1257 c         1654 g         2427 t
ORIGIN      
        1 cattggaatt atgttatttt ttatcaatgc taaactggct ttttatgttc ttgtcccagt
       61 tccgcttgtc atgatgggaa catacttttt ctggagccgg atttatccca tgaacttcaa
      121 actgtgggat agccaggcaa aggtttcaag atttttaaac atcgttcttt cgggaataaa
      181 ggtggtcaag gcattcgcac aggaagagaa ggaaaccaaa cgttttaata cgctggcttc
      241 acacctgaga gactcgcaac gcagggtctc tataagcgtc gcaaaattca acccgatgat
      301 ggcgtttttg ttcagcacag ggggtcttat tgtctggtac gccggaggta aggcagtatt
      361 acagaaccag ggttttaccc tcggacaatt gatggcattt ttgagttata caggcatgtt
      421 ctacgctccg ctttcccaga tggcattgat gtcaaactgg gtgagtagtt ttgttacagc
      481 aagccataga attttcgaga ttcttgatac agagccccag ttaaaagatg caccaggagc
      541 gatcaggatg ccccatataa ggggacatat ccggtttgag catgtaacat ttggatatga
      601 tccctatcaa ccaattataa aggatgtttc ttttgaaatt gagccagggc aactggtagg
      661 cattgttggt aaaagtggat caggcaagac gacactggta aatcttgtat gcagatttta
      721 tgatgttcag gagggagcaa tctacatcga tggcaaggat atacgtgaaa tttcttctta
      781 tgatttgcga aaacagattg gactggtgct tcaggaaccg tttcttttca gagcaagtat
      841 cgcagaaaac attttgtacg gaaggcctga tgccacattt aaggacatat tgaatgcttc
      901 aaaggctgca aactgccatg atttcatcat gaaacttccg atgggatatg atttatatct
      961 cagagaacat ggtgcaggga tatcaggtgg tgaaaaacag agaataggaa tagcacgagc
     1021 ccttttatgc gatccaccaa ttttgatact tgatgaagcc acatcatcag tggatacaga
     1081 atctgaaaag aagattcagg atgctcttgc gattctgtgc aaaaatagaa ccacaatagc
     1141 ggttgcacac cgtctttcaa ctctaagggg agcggataaa attcttgttg tggacaacgg
     1201 aaaaattgtg gaaagcggga cacatcagca attgatggat aaaaagggcg tgtactataa
     1261 actcgtcatc atgcagacca agttgacaag catagaaaca gaggcggaat gacatgggcg
     1321 aagacataaa aaaaccggaa atcgtttttc tcgatccaaa gaaaataaaa atatttcgtg
     1381 gtacattcga aacaattcat ttaatgcttg aagatggttc attatacaga ggtgtttttg
     1441 ctgtcgcggc ttttcctgtt tcaaatccaa ataaatatat ttcacttttt tgttacgacg
     1501 aaagggacag agagtacgaa attggaatga ttgaagatat tgagccatta cctgaagagg
     1561 caaaaaaact gattgttgac gcattaaaaa ggcagtattt ctcatttgag attctggcga
     1621 taaattctat caagttcgct tatggacttc ttttctttga tgttgaaact gataaggggc
     1681 ccagagaatt ttcgatgaag tgggagacaa gatttgcgtt tgatttcgga agaaagggaa
     1741 aaattcttgt tgacattttt gaagacaggt atgttatcag agatatttct aaacttaata
     1801 aaatagaaca ggaactattt acacgttaca tttactggtg acatgttttt tattggaaaa
     1861 attcttgtta gttctgaaat cgttacatgc agattttcat gcgatatcaa tgaatgcaaa
     1921 ggtatgtgtt gctgcgaggg tgaagcaggc gcacctcttg aatacagcga gattgaaatt
     1981 ttaacaaaaa acatccctgt cataaaaaat tacctgaaaa agtcctctgt ggataaaata
     2041 aacagggatg gatgttttgt aaagaatttg tggggtgggt ttgagacacc cattgctgac
     2101 aatggatggt gtatatatgc tgtggaagaa aatgggatag tattgtgtgg aatcgagatg
     2161 gcgtggaaaa ataaacagat tgatttcagg aaaccggtct catgtcatct ttacccgata
     2221 aggataagga aatttggtat ttttgaagga cttatttacg aacgatggga tgtatgcagg
     2281 tgtaagacgt tgcaggaatc tccttttttg attgagtttg cttctgaagc attggagaga
     2341 aaatatggaa atacatttgt tgagaaattg agagagattt ctatgaagga aaacacatga
     2401 ccgaagttgc gccagttttg ttcattctta ccatcactct tgttgctgtt gaagcatggt
     2461 ggatatggtt tatgtatcct catgactggt acctgaattc cggaggtttt gccttccttg
     2521 aaattttgac tactggatgt cttgtgttat ccttttttag ttttgccggg aaatttccga
     2581 ggaattttat tttttttgcg cctgttattc ctgcagtggc atgctttact gtcagaaatc
     2641 tttataagtt gtttgaagat agatatgaag ggaaaaagat gaaaacagaa atcgacatgt
     2701 tgataagaaa tgccggtaaa tttggtaatg gctatacttt tgagcaaatt ggggatatct
     2761 acttttcaag acgtgatttc gagaatgcgc tctcctggta tctgaaagcc agttctgcac
     2821 aggaaagcac tgaaatttct cacaaaatca atataacaag gcaggagatt cttctgaaga
     2881 agaaaaaact ctggatttgt cctgaatgca gtatgacaaa ttctacaaac tcagggagtt
     2941 gtaaatcctg tggtgcattg agaccatcaa taaaaacatt aaaacacgag gtgcagcgta
     3001 ccacaccgta tttaagaaag tacctgtttg tctttgcgtg gttgtttctg ttcatttcct
     3061 tctttacctg gtttataaaa aatgccaact tttttacatc atttattgtg ttttttgtgc
     3121 tatttgtgat tctggcttta tattttctgt acagactttt ttcaaattag aaaaggagaa
     3181 agaatgaatt caaaaacaag ggttatgaat acagtcctca gaacaaatat agataaaatt
     3241 cctcttgata tatgggcgac tgatgagaca attaaagcac tttgcaatca ttgttctgta
     3301 tcttatgaaa aactactgga aaaattggat atagatatca gatatattga cttacagata
     3361 gaaaatgaaa attttaaaaa aatgtatgat ggaagttttc aaagacagat tgatacgcat
     3421 tattttgttg atatctgggg cgtgataagg aagaaaataa gttacgggaa aggtgaatat
     3481 tttgaactcg ataaatctcc tctggcagag ataactgacg caaaagacgt tgatttattt
     3541 caattaccgg acccgggaga tttcgacatt gcgacagacc accttgaatt atatgacaga
     3601 tttgcaatcg tttttacagg tgacagattg accacacgca caagtttttt taagcttgca
     3661 atgtacctga ggggatttga gaattttctt ctggatttac acataaacaa gcaaattgcc
     3721 ggcgcaatta tagacaaact ttttgagttc cattataccc tcaatgagtc aatattcaaa
     3781 agatacgccg ataaaatcga cgttcttctg cttggagatg attttggtac tcaaactggt
     3841 ccattaatcg ggctggaaag tttcagagag tttttcaaac gacccttgcg tgaaatgatt
     3901 gaactctgtc ataagtataa catcaggaca atgttgcatt catgcggaag tgtaaagatt
     3961 tttatccctg acctgataga aattggcctg gacattttga accctgtgca acacacggca
     4021 tttgaaatgg acttaaggga cttgaaaagg gaattcggaa aggatatttg ttttcacgga
     4081 gcaatcgatg ttcaaaaagt gctacctttt ggtagcaaaa aagacattga aaatgaagtg
     4141 aaaacctgta ttgaaattct tggggcaaac ggtggttata tctgtgcacc gtcgcacaat
     4201 ctgcagtccg atgtccctgt tgaaaatatt atttatatgt atgagtacgc gcgttctata
     4261 aggtaaaatc tcagatgata ctaaatgtta ctttggtaca accattgaat atggtgttgg
     4321 tagttgcgat gttctcgaat caatccggtt attctcatgt ggtgagtacc agtagagttt
     4381 gaatacattt ccatgatgat ggtagagtgt gatgatggca cacaccagaa cagaatatca
     4441 ggtcttttta tgtaagaaaa aatgtatgct ggtaatattg agaatacttg acaacttata
     4501 ttgagcaatt aaactaaaac cattacacaa agggatcttt aatgaaaata tcatgttgta
     4561 ttttatccgg actttttatg attctgagcg ttgttgtggc tgggcaaaca attgaacaac
     4621 gaaccattaa tgttgacgga atcaaaagaa tctatagaat tcaaatacct gcgtgttaca
     4681 attttgacag aagttttcct cttgtttttg ttttgcatgg tggaggtggt tctggtgaaa
     4741 ccatatctaa ttttacagga tttaaaatgc ttgcagaaaa aaatagattt attgttgttt
     4801 atcctgatgg atatcaaaag caatggaatg atggcagaga ttattctgtt acaggggtag
     4861 ctgatgtaaa atttttgaga gcagtttttg aaatcgtatc acaggaattc aatattgata
     4921 aaacgagagt atatgcagca ggtatttcaa atggtgccat gatgtgttac agattgggaa
     4981 ttgagatgtc tgatattttt gcagcaatcg cagcagtttg cggaaatctc cctacaaaac
     5041 ttgaagaaaa aaaacctgaa catccggttt cagttcttat tatgaatggc acttctgacc
     5101 cgcttgtgcc gtatgaaggc ggacaggtga aggtgtttgg aaaaaacaga ggtagcgtaa
     5161 tttccactga acggactgta gaattctgga ttaaggcaaa caaatgtttt gaaaaacttc
     5221 ctgataaagt cattgatgtt gataaaaacg atggttcatt catatctgtt tattcttatg
     5281 tcaataacag aacacaggtt gttctttaca agattattgg aggcgggcat acctggccag
     5341 gtggcgttca acatcttcct gagaaaatta tcggtaaaac aaatagagat ataaaagcag
     5401 aacacgtgat atgggatttc ttttccagat gtaaaaagga gacgcaatga ctacgagtgc
     5461 aagaaagctg agagatattt acgggagaaa accatatgcg ccgtttttcc aaaaagaatt
     5521 tggttttttt acccttgaga gatggaaaaa ggaaggaaaa attgatgata ataccaatct
     5581 tgatgaactc tttggttttg atccacctgg taattataat ctcgcccagc ttggctggac
     5641 aattgctcca tttgaacccg catttgaaac aaaaataatt gaagataaga ttgattatga
     5701 acttgtccag gacgaatctg gaaggcatgt gcttttcttc aaaggaagaa gaaacggctt
     5761 tatgccagaa tatgttgcgc atccggtaaa ggactggaag acatggatcg aaaatgttaa
     5821 gtggcgcctg gatctacgga cagaaaccag aaggaaacat tttctgaaag taattcctga
     5881 attgattgat gcccagaata aaggtttgat gattacagaa cgagtgattg gcggttatat
     5941 gtatttaagg tgtctgattg gtccatctga acttccccta tttttttatg accatcctga
     6001 tgttattcat gattgcatga aaacatggtt tgaacttgca aatgccatca ttgaaatgca
     6061 ccagcagtat actgtaatcg acgaattatt ttttggggaa gatatatgtt acaaccatgg
     6121 tccactgatt tcacctcaaa tgattaaaga gttccttttt ccttactatc aacaactcat
     6181 tacaaatatt aaatcacgac aggcaaaatc aggccggcat ctttatttcc atcttgatac
     6241 tgatggttac gctgtttctg ttattcccct ctataaagaa cttggtatgg atgtgatgag
     6301 tccatttgaa gttgcatctg gttgtgatgt tgtgagggat ggtcagacat ttcctgatat
     6361 tgcaatgttt ggtggcattg ataaaagggt tcttgcaaaa acaaaagatg agattgacag
     6421 acatcttgac aggattttac cagcaatgta caaaagaggt gggtatattc ccacatgcga
     6481 ccacggagta ccagaagaag tttcttttga aaattacatg cattacagaa aacgaatttt
     6541 agaatataat tagtgattat gaacagaaaa aatgtcgtga tcaaattttt tgctcctgtg
     6601 atggacgcaa gtattaatgc gttgatgaat gcaatcgacc agaaaatgcg ggaaggaata
     6661 tcaaaattca caattttgat ttcttcacca ggtggttctg tatttcatgg tttgagtgca
     6721 tacaattatt taaaagggat tcctgcagaa attataactc acaattttgg aagcgttgat
     6781 tctattggtg tggtattatt ttgcgctggc gaaaaacggc tttcagtacc gcaggcaaga
     6841 tttttattgc atggcgttag cgtggcattc aggcagaatg aaaatcttga agaaaaacaa
     6901 cttgaggaac gattgaaagg cctgcgtatt gatattgaaa atatcgcaaa aattatttct
     6961 tcgaattcaa acaaaggtat aaatcaggta atttctgcaa tgcttgaaag aaccacattg
     7021 aacccacaac aggcaattga atggggtctt gtacacgaaa ttgtacctga actttatgaa
     7081 tcttgttcag atgttatttc tatccatttt caatcacatc aaacctgata gttaatcttt
     7141 tatttcaatg cgaccatacc acatttcatt gtcaggattt gtgatatgcc cataaatatg
     7201 aatgatatca gaaggaaaag aaccattcct tatactggca gtgtgaaaca aagtgaaagg
     7261 tttttcaaaa ttcacaattt ttttatcaac aatattaccc gtggaatcaa gtgaaatcag
     7321 gtaattttca gcaacagtgt tattgttctc gtcttttcca tgtaatgaag caaaaacaag
     7381 caatttccct tttcttgtta tatgaaacct gccccagaaa aagttgatat ttttcatgcc
     7441 acagggatat tgttctggat actgttctga aaacaataga atcgtttttc tttcaagcac
     7501 tttttgcttt ttcaattgag catattcaag agaataagtt agaatctcgt cgggaaaaaa
     7561 ttgttccctt aacctctcgt cgcaactttt ttccatccag agtaaatgaa ttatatcatc
     7621 gttatctgca tacaggtcct gatttctggc attacctgct gttttttctc tgttagatat
     7681 ttcagaccag tatctgaatg gttcttttgt aatgtctggg gtaaaagaaa aaaatatcct
     7741 tctgaaatca taatcccact ctcttgctgt aatttttctt ttatattgcc tccattccg
//
LOCUS       DAORYU010000044         7794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_6680_length_7794_cov_4.865874, whole genome shotgun sequence.
ACCESSION   DAORYU010000044 DAORYU010000000
VERSION     DAORYU010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7794
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6680_length_7794_cov_4.865874"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..1055)
                     /locus_tag="PLW07_02420"
     CDS             complement(126..1055)
                     /locus_tag="PLW07_02420"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOL49409.1"
                     /translation="MNNCLVFTGNANRPLAEKICDFLEKPLGQMEVSRFNDGEISVKI
                     EENARGKDVFIIQPTCAPVNENLMELLIILDAFRRASPRRITAVVPYYGYARQDRKDR
                     PRVPITAKLVANLLVAAGAQRILTMDLHAPQIQGFFDIPVDHLFAAPVIINYMKSKKL
                     TNPVIVAPDVGSVKMARAFAKRINARLAIVDKRRDNPDSVEATHLIGNVSNCEAIIVD
                     DLISTGNTIIEATRVLLENGASKVFVSCTHPVLSGNSITKLTGAGITEIIVTDTIPHQ
                     SLPENFTVLSVAELLGEAIRRIHQETSVSSLFI"
     gene            complement(1052..1813)
                     /locus_tag="PLW07_02425"
     CDS             complement(1052..1813)
                     /locus_tag="PLW07_02425"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOL49410.1"
                     /translation="MKKTMNNHMAVIILAAGYGKRLGGENQKTLTEILGKPLIAYLIS
                     TIRQCTPEKVVVVVGFQKEKVMEQLKGEPVIFVEQKQLLGTGHAVMMTENVFSDFCGN
                     VIILYGDVPFLSLETIKRLKETHEDTQSDCTILTAIVEQPYGYGRIVRDSSKNVIKIV
                     EEINASDEEKQIREINSGVYIFTNKTLFEALKKIQPNPVKNEYYLTDVIEIFISEGKK
                     VATWTTPCPGEIIGINTPEDLEKAQQYILRLRSIQ"
     gene            complement(1819..1889)
                     /locus_tag="PLW07_02430"
     tRNA            complement(1819..1889)
                     /locus_tag="PLW07_02430"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1855..1857),aa:Gln,seq:ttg)
     gene            complement(1907..2299)
                     /locus_tag="PLW07_02435"
     CDS             complement(1907..2299)
                     /locus_tag="PLW07_02435"
                     /inference="COORDINATES: protein motif:HMM:NF015959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVG family protein"
                     /protein_id="HOL49411.1"
                     /translation="MEITETRISPINKPNSRLRAYASVTFDNSFVIREIRIIEGKNGL
                     FIAMPSRKMQKPCPKCNFKNPVTNKFCGQCGAEIKNVEQKNQKTGPQQHKDLAHPINT
                     QFRDYLQKQILEAYSQQKESTTGKENNI"
     gene            complement(2286..3188)
                     /gene="ispE"
                     /locus_tag="PLW07_02440"
     CDS             complement(2286..3188)
                     /gene="ispE"
                     /locus_tag="PLW07_02440"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOL49412.1"
                     /translation="MEKIRILAPAKINLFLEVTGKRTDGYHNLCSLVEKVNLTDEVEI
                     CLDEKHRVEFSGIWQIPKENTVTKTVHELSMLFPDIVKKNPVKIVIKKCIPPGSGLGG
                     ASSDAAAVLKGCNELWKLGLDQDKLIKIGSKIGSDVPLFLKEGPCIIEGRGEIVHTTG
                     MLPSLFFHLFVPEFQVSTKLIYDNLSSDLLTDLTSARSRIKIFVSLWKENNKKQMEKF
                     LFNKLEEVTRRIYPEIDRAIRRLEALSGKRFVLTGSGGGIYALGKMDQLLLDRIPEII
                     RHWRYYKLESFCTCLQKEEQHGNY"
     gene            complement(3151..3756)
                     /locus_tag="PLW07_02445"
     CDS             complement(3151..3756)
                     /locus_tag="PLW07_02445"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOL49413.1"
                     /translation="MRNMDKNNKEKIIHIREKDFRRIAAFRSKARKIENKCTALEEKI
                     AGMEKELSEWKERATRLAAEMENLRKRIEKEKQEFSKFIQASFAEKLLFFDEIFEKVV
                     NDVCSDKDINKNVADGLVMLKKQFTSLLESLGVQKIETQGKVFDPMIHEAVETVETDE
                     KPEGTIIEEVRAGYTINGKLLKPAQVKVVRGKNKDTGSSKN"
     gene            complement(3753..4331)
                     /gene="lepB"
                     /locus_tag="PLW07_02450"
     CDS             complement(3753..4331)
                     /gene="lepB"
                     /locus_tag="PLW07_02450"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOL49414.1"
                     /translation="MRQNKNREKTRPSSKMQKRKKEILSWVWAIIFVLVLRQFFVQAF
                     VIPSESMKPTLKIHDRLLANKFIYKIREPHRWEVIIFKYPEDPSKYFVKRLVGLPGES
                     LLIKNGNVCINGISMKQPDYVSTSLYYYSDIDASFGVREEIKIPASAYYVLGDNSINS
                     RDSRYWGFVPSKNLVGKPFFIYWPPWRMGLIR"
     gene            complement(4338..5453)
                     /locus_tag="PLW07_02455"
     CDS             complement(4338..5453)
                     /locus_tag="PLW07_02455"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOL49415.1"
                     /translation="MKNKILYILPSKDIGGTEKMVVMMAANILDYGFQPVVLTLQEKG
                     SFHTLLDAHHIKHYSLNIKKRPLCALFKIFFVIFKEKPCLVHSFLFAGNVFAGMLRIF
                     LWKPLVCSQRSTDFWKKNIHWKLEKFATSFCSLFISNSNAGKKILIEKADIPEKKIIV
                     IPNGIDLTGVKDKMKSTTINPHIGIIVGSIGSLRKAKGYDVLVDAAVIVCKKRKDVTF
                     VILGKGPLEQVLKEKIQHLGLARNFEFHGFVENVYTYLPTFDIVVIPSLWEGFPVVAL
                     EAMACGKPIVATRVGDLPEILQHRISGLLVETGNSEEMANSILMLAGDENMRIWMGNN
                     AMKQVEKFSATGMLTQYSNVYYQLLSKHETKTAANTS"
     gene            complement(5443..6057)
                     /gene="tsaB"
                     /locus_tag="PLW07_02460"
     CDS             complement(5443..6057)
                     /gene="tsaB"
                     /locus_tag="PLW07_02460"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOL49416.1"
                     /translation="MYLFSIECSTQRVGIAVVQGKNLLVKKTWLSRDTSTEILPEIDR
                     MLKKVGLQPEQFDYFVVSTGPGSWTGVRLGLALAYGLAIADEEKVFGLSGLEAMAYQL
                     KNENNVGVFLPSVKNSVHFGFFKNPCSLEKKYGKLSSCAIKQLPEKLKNAQIIAGPDR
                     KICSLFNPRKPFVEIFPDPVLNAMLAFDRIQNSVKPENQPYYEK"
     gene            complement(6061..7161)
                     /locus_tag="PLW07_02465"
     CDS             complement(6061..7161)
                     /locus_tag="PLW07_02465"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOL49417.1"
                     /translation="MVALLISGCSENRDIVIKKTENIPAENISEETIPMVVMVPSEKY
                     EPAGETKVSVEKSVVKPGVKPQEVEKTSSDTVLSECVSNQTETVEAKTMEQKNAGTKK
                     PLEKNKTPEITISKSPLLIIPGECLVFRIKWNFANVGKLILACKKEKIDNRDVYHFVG
                     LTVPEGIWTKLGNGYNRFDSYIDSKTNLPFYYYQYSASSSTSQVTKSIIDHRTNTLSY
                     EVKKYKNNKQYGAKSGKITFSGILIDGLSALYAIRGIAGDQMISSKMPVGITKITDIY
                     LFFVGKNTDNFVVGKREYWLIQSEASEDEAIFRKGKLFVSISADKEKLPLLMKGKVPL
                     GTGTVELISRKTLDTNFSTDSKSLTEILNSTL"
     gene            7361..7756
                     /locus_tag="PLW07_02470"
     CDS             7361..7756
                     /locus_tag="PLW07_02470"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOL49418.1"
                     /translation="MRTAKWIIDIENLAKNAGHFKVKDLLDLTENSGMNVSRATVYRV
                     INKFLNTGKIVQISSEKERCFEFVGQKLHYHFKCKKCEKIMEFFSDEIEKSIREITKK
                     LGVVMTEQKLIIEGICQRCARGKDVRRKR"
BASE COUNT         2187 a         1556 c         1403 g         2648 t
ORIGIN      
        1 gtataataag caggtcaatt atttaacttt attaaaaaga gggaaaaatg agaaggaaaa
       61 aaagaaaact caggtggaga tcaaaaaggg ctaaccacag aaaaaaacct tccatggggt
      121 aacattcaaa taaacagcga gcttacagaa gtttcctggt gtattcttct tattgcttcg
      181 ccaagcagtt ccgccacaga aagaacagta aaattttcag gcaatgattg atggggaatt
      241 gtatcggtta caataatttc tgtaattccg gcacccgtca gttttgtaat tgaatttcct
      301 gaaagcactg gatgggtaca gctgacaaat acttttgacg ctccattttc aagaagtact
      361 ctggttgctt caatgattgt attacctgtc gagattaaat catcaacgat gatggcttcg
      421 cagttagaaa catttccaat gagatgggtt gcttcaactg agtcgggatt gtcccgcctt
      481 ttatccacta ttgccagccg tgcattgatt cttttcgcaa acgcccttgc catttttacg
      541 cttccaacat cgggtgctac aataacagga tttgtcagtt ttttgctctt catatagtta
      601 atgataacag gtgcagcaaa aagatggtca acaggaatat caaaaaaacc ctgaatctga
      661 ggtgcatgca ggtccatcgt caatatccgc tgggcgcccg cagctacaag aagatttgcc
      721 accagttttg ctgttatggg aaccctgggt ctatcttttc tatcctgccg cgcataacca
      781 taatatggta caactgctgt aattctgcgg ggagatgccc tgcgaaaggc atcaagaata
      841 atcaagagtt ccatgagatt ttcgtttaca ggagcgcagg ttggctgtat aatgaaaaca
      901 tcttttcccc ttgcattttc ttctattttc acagaaattt cgccgtcatt gaatcttgaa
      961 acttccattt gtccaagcgg tttttcaaga aaatcacata ttttttctgc cagaggtcta
     1021 ttagcatttc cagtaaaaac cagacagtta ttcattgtat gctccttaac cttaaaatgt
     1081 attgctgcgc tttttccaga tcttcagggg tattaattcc tattatttct ccaggacacg
     1141 gcgttgtcca tgttgcaact tttttccctt ccgatataaa gatttcaatt acatcagtaa
     1201 gatagtactc attttttaca ggatttggtt gtattttttt tagcgcttca aagagggttt
     1261 tatttgtaaa aatgtagacc cctgagttaa tttctcttat ctgtttttct tcgtctgagg
     1321 cattaatttc ttcaacaatt tttataacat tttttgatga gtcccttaca atcctgccat
     1381 atccatatgg ctgttccaca atagcagtaa gaattgtgca atcagattgt gtgtcttcat
     1441 gggtctcttt caaccttttt attgtttcaa gagacaggaa aggcacatct ccgtacagaa
     1501 taatcacatt tccacagaaa tccgaaaaaa cattttctgt catcattact gcatgacctg
     1561 taccaagcaa ttgtttttgt tctacaaata ttacaggttc tcctttcagt tgttccatta
     1621 ccttttcttt ttgaaatcca acaaccacga caactttttc cggagtgcac tgtcttattg
     1681 tggatattaa gtatgcgata agtggtttgc caagaatttc tgttaatgtt ttttgatttt
     1741 ctccaccgag ccgtttacca taccctgccg caagtattat taccgccatg tgattattca
     1801 tagttttttt cacaatacct gcccggcgag gatttgaacc tcgaaccacg gctccaaagg
     1861 ccggtgtgtt accgttacac caccgggcaa aatcattcaa ttatttttaa atgttattct
     1921 cttttccggt ggtgctttct ttttgctggc tatacgcttc aagaatctgt ttctggagat
     1981 aatcccggaa ttgagtattt atcggatgag caagatcctt atgctgttgt ggcccagtct
     2041 tctgattttt ctgttccaca ttttttattt ctgctccgca ctgtccgcaa aatttgtttg
     2101 tcaccggatt tttaaagtta catttgggac atggcttctg catctttcta ctgggcattg
     2161 cgataaaaag gccatttttc ccttcgataa tccgaatttc acggattaca aaagaattgt
     2221 caaaagtcac cgacgcatat gcgcgcagtc tgctatttgg cttattaatt ggcgaaattc
     2281 tggtttcagt aatttccatg ttgttcctcc ttttgcaggc aagtgcaaaa actttcaagt
     2341 ttatagtaac gccagtgccg gattatctcc ggtattctgt ccagaagaag ctggtccatt
     2401 tttcctaaag catagatgcc tcctcctgag ccggtcagaa caaaacgttt tccgctcaat
     2461 gcttcaagac gtctgattgc cctatctatt tccgggtaaa ttctgcgcgt cacctcctcc
     2521 agtttgttga acagaaattt ttccatttgt tttttattat tttccttcca taatgacaca
     2581 aaaattttaa ttcttgaacg ggctgatgtc aaatcagtca aaaggtcaga agaaagatta
     2641 tcataaatca atttcgttga aacctgaaac tctggaacaa aaagatgaaa aaaaagcgaa
     2701 ggcaacattc ctgtggtgtg tacgatttca ccacgacctt ctattataca gggaccttct
     2761 ttcaaaaaca ggggtacatc agagccaatt ttactgccga tttttattaa cttatcctgg
     2821 tcaagtccga gtttccagag ttcattgcat cctttcagta ccgcagcagc atcactgctg
     2881 gcaccgccaa ggccgcttcc aggcggaata catttcttga taacaatttt tactggattt
     2941 tttttcacta tgtctggaaa taacatagag agttcgtgca cagtttttgt cacagtgttt
     3001 tctttgggta tttgccagat tcctgaaaac tcaacacgat gtttttcatc cagacatatt
     3061 tctacttcat ccgtgaggtt tactttttct acaagggaac agagattatg ataaccatcg
     3121 gttcttttgc ctgtgacttc gagaaataaa ttaatttttg ctggagccag tatccttatt
     3181 ttttccacga accaccttta cctgagcggg tttgagaagc ttgccgttga tggtgtaacc
     3241 ggcgcgaact tcttcaataa tcgttccttc tggtttttcg tctgtttcaa cagtttcgac
     3301 tgcttcatga atcataggat cgaacacttt tccctgagtt tcaatctttt gtactccaag
     3361 ggattcaagc aatgatgtga attgtttttt aagcattaca agaccatctg ccacattttt
     3421 gtttatatct ttatctgaac aaacatcgtt tacaaccttt tcaaaaattt cgtcaaagaa
     3481 aagaagtttt tctgcaaaag acgcctgaat aaatttactg aattcttgct tttctttttc
     3541 aattcgtttc ctgagatttt ccatttcagc cgcaagtctt gtagctcttt ctttccattc
     3601 cgataattcc ttttccattc ctgcaatttt ttcttcaagc gccgtgcact tgttttcaat
     3661 ctttctggct ttgcttctga aagcagcaat tcttctaaaa tccttttccc tgatgtgaat
     3721 aattttttct ttgttatttt tgtccatgtt tctcatctga tcagtcccat tctccatggt
     3781 ggccaataaa taaaaaatgg cttaccaacg agattttttg aaggaacaaa accccagtat
     3841 ctgctatcac ggctattgat actgttatcc cctaatacgt aataagcaga cgcaggaatt
     3901 ttgatttctt cacgcacccc gaatgaagca tctatatcac tgtaataata aagggaagtt
     3961 gacacataat ccggttgttt catgcttata ccgttgatac atacgttacc gttttttatc
     4021 aagagagatt cccctggaag accaacaagt cttttgacaa aatattttga aggatcttca
     4081 ggatacttaa aaatgattac ctcccagcga tggggctccc tgattttata aatgaatttg
     4141 ttagcaagaa gtctgtcatg aatttttaaa gtgggtttca ttgattcact tggaatcaca
     4201 aatgcctgaa caaaaaactg tctgagaaca agcacaaaaa taattgccca tacccaggaa
     4261 agaatttcct tttttctttt ctgcatcttt gaagatggcc gggttttttc tctgtttttg
     4321 ttctgtctca tattatttta agatgtgttt gctgctgttt ttgtttcatg ttttgaaagc
     4381 aactgataat aaacattgct gtattgcgtt agcatacctg tcgctgaaaa cttttccacc
     4441 tgtttcattg cattattccc catccagatt cgcatgtttt cgtctccagc aagcattagt
     4501 atggaatttg ccatttcttc agaattgccc gtttcaacca gtaaaccaga aatcctgtgt
     4561 tgtaaaattt ctggtaaatc cccaaccctt gttgcgacaa ttggttttcc gcacgccatg
     4621 gcttccagtg caacaaccgg aaagccctcc cacaaagagg ggatgacaac aatgtcaaat
     4681 gtgggcaagt acgtatacac attttccaca aaaccatgaa attcgaaatt tcgcgcaagc
     4741 ccgagatgtt gaattttttc cttgagtacc tgctcaagcg gtcctttccc aagaatcaca
     4801 aatgtaacat cttttctttt cttgcacaca atgactgcag catcgacaag gacgtcatat
     4861 cctttggctt ttcttaaact accaatggac cccacaataa tcccgatatg cgggttaata
     4921 gttgtgcttt tcattttatc ttttaccccg gttaagtcaa taccgtttgg aattacaatg
     4981 atcttttttt ctggaatatc tgccttttca atgagtattt ttttgcctgc gtttgaattg
     5041 gaaataaaca atgaacaaaa tgaggttgca aatttttcaa gtttccagtg tatgttcttt
     5101 ttccagaaat cagtacttct ttgagaacaa acaagaggtt tccagagaaa aattcttaac
     5161 atgcctgcaa aaacatttcc ggcaaaaagg aagctatgta ccagacaggg tttttcttta
     5221 aaaatcacga aaaagatttt aaaaagtgca caaagcggtc ttttttttat attgagagag
     5281 taatgtttta tgtgatgagc atcaagcaat gtatgaaacg aacctttttc ctgtaatgtc
     5341 aatacgactg gctgaaaacc ataatctaat atgttggctg ccatcatcac caccattttt
     5401 tcagttccac ctatgtcttt gctcggtaaa atataaagaa ttttattttt catagtatgg
     5461 ctgattttct ggtttcacag aattttgaat tctgtcaaat gctaacatcg cattcagaac
     5521 aggatccgga aatatttcca caaatggctt cctcgggtta aacaaagaac aaatttttct
     5581 gtcaggcccg gcgataattt gcgcattttt aagtttttca ggtaattgct taattgcgca
     5641 tgatgataat tttccatatt ttttttcgag agaacaagga tttttgaaga atccgaaatg
     5701 aacagaattt ttcaccgaag gcagaaaaac accaacatta ttttcgttct tcaattgata
     5761 tgccattgct tcaagaccac tcaatccaaa gactttttct tcatcggcta ttgcaagtcc
     5821 atatgcaagc gcaagaccaa gtcgtacacc tgtccaggaa ccaggtccag ttgaaacaac
     5881 gaaataatca aattgctcgg gttgtaatcc gactttcttg agcattctgt caatttcagg
     5941 gagaatctca gtgcttgtat ccctgctgag ccaggttttc tttacaagga gatttttgcc
     6001 ctgtaccaca gcgataccaa cgcgctgtgt tgagcattca atagagaata gatacatggt
     6061 ctaaagagtg gagttcagga tttcagttag acttttgctg tctgttgaga aatttgtatc
     6121 aagcgttttc cgtgagatga gttccactgt tcctgtcccc agtggaacct ttcctttcat
     6181 aagcaatgga agtttttctt tgtcagcaga aattgaaaca aaaagttttc cctttctgaa
     6241 aattgcttcg tcttctgatg cttctgattg aattaaccag tactcccgtt taccaacgac
     6301 aaaattatca gtattttttc ccacgaaaaa taagtagata tcagtaattt tcgtgatgcc
     6361 gaccggcatt tttgaagata tcatctggtc tccagcaata cccctgattg cgtaaagtgc
     6421 tgatagccca tcaatcagta tgccagaaaa ggttatctta cctgatttgg cgccatactg
     6481 cttgttattt ttgtattttt ttacctcgta tgaaagtgtg tttgttcgat ggtcaataat
     6541 actttttgtg acctgacttg ttgaagatga tgctgaatac tggtaataat aaaaaggaag
     6601 attggttttg ctgtctatgt atgaatcaaa tctgttgtat ccattgccaa gttttgtcca
     6661 tattccttcg ggtactgtaa gacctacgaa atgataaaca tcccggttat ctattttttc
     6721 ttttttgcac gccagaatca attttccaac atttgcaaaa ttccacttta ttctgaagac
     6781 aagacactct cctggaatta tcagtagtgg actttttgat atcgtaattt ccggagtttt
     6841 gttcttctct aaaggttttt tagttcctgc atttttctgt tccatggttt ttgcttcaac
     6901 agtttctgtc tgatttgata cacattcgct caaaacagta tcagaagacg ttttttcaac
     6961 ttcctgtggc ttgacaccag gttttacaac ggatttttca acagatactt tggtttcacc
     7021 tgccggctca tatttctcag acggaaccat cacaaccatg ggaatggttt cttcagaaat
     7081 attttctgcc gggatgtttt ctgttttctt gataacgata tctctgtttt cagagcaacc
     7141 gctaataagt aaagcaacca ccaggattat aaataccgtt ttgtgtttca tttttggaaa
     7201 ttttttggta ttttatttta tcataagacg atttttaatg ccagaggttt atttcctggt
     7261 atgctaattt tacacagttt cctttttgct ggcaagtgga ttgactttgt ttattctgtc
     7321 tgctaaacta agtgagattg aatctcaaaa aggatgatta atgagaactg ctaagtggat
     7381 tattgatata gaaaatcttg caaagaatgc ggggcatttc aaagtgaagg acctgcttga
     7441 cctgacagaa aattcaggta tgaatgtatc acgtgcaact gtgtatcgtg taataaataa
     7501 atttctcaat acagggaaaa ttgttcagat ttcaagtgaa aaagaacggt gttttgagtt
     7561 tgttggtcag aaacttcact atcattttaa gtgcaagaag tgcgaaaaaa taatggaatt
     7621 tttttctgat gaaatagaga aatcaatcag ggaaattaca aaaaaacttg gcgtagtgat
     7681 gacagaacaa aaactcatca ttgaaggtat atgccagaga tgtgccaggg gaaaagatgt
     7741 caggagaaaa agataaagtg aagcaaccgc aggatgaaaa aaaaattatt cttg
//
LOCUS       DAORYU010000045         7764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_6718_length_7764_cov_4.466987, whole genome shotgun sequence.
ACCESSION   DAORYU010000045 DAORYU010000000
VERSION     DAORYU010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7764
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6718_length_7764_cov_4.466987"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1115
                     /locus_tag="PLW07_02475"
     CDS             <1..1115
                     /locus_tag="PLW07_02475"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOL49419.1"
                     /translation="QVIVGGITHEFLHMLEKFGGAWMRDEKTHSMVFDPESMIMVSQE
                     MVLESGCTILFHTWICEPIVENGVVKGVITENKSGRQAFLGKVIIETTGDGDIAARAG
                     AQFEFGHGSKDILQPVTLEVIVGNVDVTKISLDAFKKIKTWIAEAKQNNEWIIPTERF
                     FSWGRVIKRGEPANPKSSFFFFNATNVLNINGCDVEDLTRAEIESRRQVDCLIRFLRK
                     YVPGFENCYPDRTAAQVGIRETRRIIGDYILKREDVLSGRHFDDGVVPSCNSIDVHDT
                     EGKDFKHEFLNPDAHYEIPYRCFLPKGLDGILTGGRCISADYRALGSTRVMVVCMPIG
                     EAIGIAASIAVKENCTPREIPVQKLRSALRNAGMSL"
     gene            1625..2668
                     /locus_tag="PLW07_02480"
     CDS             1625..2668
                     /locus_tag="PLW07_02480"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOL49420.1"
                     /translation="MSKIKLGLIGVGWIGSEHGRNILANKKAELVGVADPDSKKIAIF
                     FKNQNYECQHYSDYRDLLDAEVDAVVIASPNNMHATMCIDAAKKGKHIYCEKPMAITL
                     DDCRRVRDAVTKYRVKYLIGYHRRLNPLYQFVRNLLNDGKLGNPFLIESDYIHHIPGD
                     WDIWNWAGKAELAGSLFHAGSGHNVDLIRYFCGEIKSVTCFKDIFLPRTNQLETEDTA
                     IAVFLFRNGAIGKVHFCVGPIVPFTFSFKLYGTKGTVINNKVWLDNIPLFYEPGHEND
                     CLTLPASWIPDNVQGSISEPWNKLMDHFIDMLTDDVPCVNDVESAFRTSAACFAAMKA
                     AEQKTVVDVTSLE"
     gene            2738..4015
                     /locus_tag="PLW07_02485"
     CDS             2738..4015
                     /locus_tag="PLW07_02485"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOL49421.1"
                     /translation="MLRQVFLSFFDRTNQNKGRNDMKHDFHKIDLKFGARELRQLGER
                     ELYYLRQVMEGETLSNYISPDSMTRRFEEAFAKKIGARKAMAKNSAMSGLCEAVSVSG
                     AGPGYEVICDPIVHFGGLAAIYFNAVPRFADVKRDTYNMDPDSLLANITPLTKAVIVT
                     HLWGQSAEIDTIAQICREKGIFLIEDCAHAIGATWNGQHVGTFGDLGVFSFQEFKQLS
                     TGDGGMTVTNNEELFDRMRNAWWFSGESPKFMYLNFRMNEVTAAVGLAQLEKVDRIIE
                     GTYNKTLAILNKAIEGCSWLKPRAVPKQAHMGGYWWAFTWEGDKQGLSYDKFKQLNDE
                     LEIGLRFGFNQYPAYEFPFFRQSTAYRSHPDCPVRCPFYTARSNYRYTWDLTPVCADL
                     MPRLITVNLIFLSVPAAEEMAERIRTAIKKMNG"
     gene            4042..4740
                     /locus_tag="PLW07_02490"
     CDS             4042..4740
                     /locus_tag="PLW07_02490"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOL49422.1"
                     /translation="MNILIFGAHPDDIEILCGGTSAKFKRLGHKVTHCVLTDGQVSSR
                     RIPNQELVQIRKNEAKAAADVIGVDLVMCGIKDEMLFDDEDTRLKVLDVILTVRPDVI
                     ITMSDKDYASDHKATYNLVIAVIPMSVVKNCYSQKPCLDKHPVVYMMDTIYGIDFLPT
                     EYVDISEDFDTKLKALRCHESQIEIGKELGCDYEEQVKVVSRFRGLQAGVRYAEGFQK
                     LNNWYSGVTTRYLP"
     gene            4868..6769
                     /locus_tag="PLW07_02495"
     CDS             4868..6769
                     /locus_tag="PLW07_02495"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046353 - aminoglycoside
                     3-N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HOL49423.1"
                     /translation="MIVMEEPFITKEKLVSNLKNIGVEEGDVLFIHSSFKSIGKVEGG
                     AQTVIDALKESVGEKGTILMPVFNLVKDRPGTWNVTTTPSTTGYLTEYFRTLPGTVRS
                     DHYSHSVAAWGAHAYEFVSGHRGAKGMISPWDHEQFGCTYGYESPFMKLYCHTKSKIL
                     MLGVDYHSSTFCHLVEVIFWNERLLTDQKASYVSINREKLGQYFDSLGKLTRSFIGQA
                     DSRLFKIRDFVDTLCREVRKHTDKYVSMLINMKQSISTGEAMKIKIIKKRIISKDNSY
                     HGWPSLCKRKNGQLLVVASGGRQAHVCPFGKIYLYRSDDNGNTWSGPEVLYNSPLDDR
                     DPGILETDNNTLLVSWFTSLTWMNYLYRAEIGIIKWLPEDTCKKWREIRDKLLEDEIN
                     TGRELDTWMIRSEDGGKTWSERIKIPVHCPHGPVQLKDGRLLFAGRQSLPPEKRSLYG
                     ASFIGIDRENSEIAVAESNDDGKNWKITGVLPVLPPVPPDNFCEPHLVETTSGTLIMH
                     IRNQCPSVFPGEILQSESEDGGKTWSVPHPIGLKGLPAHLLCLHNGWILTTYGYRKNP
                     FGIRASVSKDEGKTWSEPIVISDDGYNSDLGYPSSVQIDDGSIITVWYEVLKDNPFAV
                     LKMAHWVFI"
     gene            6788..>7764
                     /locus_tag="PLW07_02500"
     CDS             6788..>7764
                     /locus_tag="PLW07_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49424.1"
                     /translation="MLNPGKNALLALDTIYKKPAKGIPSWVIHPMEHSVIDYLAGEEP
                     GTYVKNPRQTYLKMQKNVGTCMIDQYIPENPLSIGSRGYEKKTETPTTGAKETIYDGI
                     KIDSPEAVVEHMEKVVSPKVKHNIETWSEDLLVQVAEKIINNEQEIQKEFGPDILKVP
                     YGVATFPGFAYGYYGYENYFMAYALYPEVMEKWFSLRADYCQLYNKAVALAYEKANLP
                     PLLRLDHDMAGEKGTLVNIRSIDKIWFPHFYHSVKELADKITLIWHCDGNLMEMVPRL
                     IECGIKGFQGFQYECGMDYEKICKMKTRDGEDLFIIAGVSVTRTLPSGTT"
BASE COUNT         2450 a         1463 c         1751 g         2100 t
ORIGIN      
        1 accaggtcat tgtcggcggc attacccatg aatttttaca catgcttgaa aaatttggtg
       61 gtgcctggat gagggatgaa aaaacgcaca gtatggtttt tgaccctgaa agcatgataa
      121 tggtttcgca ggaaatggtc cttgaatcag gttgtacaat tctgtttcat acctggatat
      181 gtgaacccat agttgaaaac ggcgtggtaa aaggcgtgat tactgaaaac aaatcaggaa
      241 ggcaggcatt tctgggcaag gtaattatag aaacaacagg ggatggagat attgccgcga
      301 gggctggtgc tcaatttgaa ttcgggcatg gaagtaaaga cattcttcaa cctgtaacac
      361 ttgaggttat tgttggaaat gttgatgtaa caaaaatttc tctggatgct tttaagaaga
      421 taaaaacatg gattgctgag gcaaaacaaa acaatgagtg gataattccc actgaaaggt
      481 ttttttcctg gggaagggta ataaagcgag gcgagccagc aaacccaaag agttcattct
      541 tcttttttaa tgcaacaaac gtcctgaata ttaatggttg tgatgttgaa gaccttacaa
      601 gggctgaaat agaatcaaga aggcaggttg actgtttgat acgttttctt cgcaaatatg
      661 ttcctggatt tgaaaattgt tatcctgaca gaactgctgc acaggttgga attcgggaga
      721 cgcgtcgtat catcggggac tatattttaa aaagggaaga tgtactgtca ggaaggcatt
      781 ttgacgatgg agtggtgcca agttgtaatt caatagatgt tcatgacacg gagggaaaag
      841 atttcaagca tgaatttctg aatccagatg ctcattatga aattccatac agatgttttc
      901 ttccaaaagg acttgatggg atccttactg gtggcaggtg catttctgct gattatcggg
      961 cactgggttc aacaagggta atggtagttt gtatgccaat tggagaagca attggaattg
     1021 ctgcatctat cgcagtaaaa gaaaactgta caccaagaga aattcctgtc cagaaattac
     1081 gctctgccct tcgaaatgca ggaatgagtt tataaaatgt tgcaattgtg ccagatgtgt
     1141 tgaagaatgg aatgtgatac acaatagtat cattgaaaac accgcgatta aaaattctgg
     1201 cgtaacctgt ctttatagcc ctggttgcag gaaaaagggg ttaaatcggg attgaagaat
     1261 aaaagttttc actgtattac aatgttttta aattgcactc tatcaattta tagggtttga
     1321 ttttaaaagg atacatagca ttcattatcg ataaaaaggc gtctcaccct acaaccattg
     1381 acgttcttga ttttcccggg attgcaccgt atcaatccgt cagcgctggc atgcaacaat
     1441 aatgtagatg cccttcaata atcattcaac atcacaaaaa ctattcaatt attctcaggt
     1501 attgcacaca aatcagtcac tttaaaaaat ttgcattttg cttcccatcc tgttagaata
     1561 gaaactgaaa aatacacgga ttctatcgtg ttcatttgta cattcaccct gaagcgagat
     1621 atatatgagc aaaattaaac ttggccttat aggtgtgggc tggattggaa gcgaacacgg
     1681 acgcaatatc ctggcaaata aaaaagcaga actggtagga gtggcagacc ctgatagcaa
     1741 aaaaattgca atctttttca aaaaccagaa ttatgaatgc cagcattaca gcgattatcg
     1801 cgatttgctg gacgcagagg ttgatgcggt ggtgattgcc tccccgaaca atatgcatgc
     1861 cacaatgtgc attgatgctg caaaaaaagg caaacacatc tattgtgaaa aacccatggc
     1921 tattacgctg gatgattgcc gtcgtgtccg cgacgctgtc accaaataca gagttaaata
     1981 tcttatcggc tatcaccgcc gtctgaatcc gctctatcaa tttgtcagga accttttgaa
     2041 cgacggaaaa ctgggaaacc cctttctgat tgaatccgac tatattcacc atattcctgg
     2101 tgattgggat atatggaatt gggcaggcaa agcagaactc gctggaagtt tattccatgc
     2161 cggcagtggt cacaatgttg accttatccg ttatttctgc ggtgaaatca aaagcgttac
     2221 ctgttttaag gatatttttc tgccacgcac caatcaactg gaaacagaag atacagcaat
     2281 cgccgtcttt cttttcagaa atggcgcgat aggcaaggtt catttctgtg tgggtcctat
     2341 tgttcccttc accttcagtt tcaaacttta tggaacaaaa ggtacagtga taaacaacaa
     2401 agtctggctc gacaacatcc cgttgttcta tgaaccaggc catgagaacg attgcctgac
     2461 actgccagcc agctggattc ctgacaatgt gcagggcagt atcagtgaac cgtggaataa
     2521 actcatggac catttcattg acatgctaac tgacgatgtt ccctgtgtga acgatgtcga
     2581 aagtgctttc agaaccagtg cggcctgttt tgctgcaatg aaagccgcag aacaaaaaac
     2641 agtggtggat gtgacatcat tagaataaat gaaaaggtgg tgtcaaaaag gaataaaaga
     2701 aggatgaacg gaaagggaac aaactggctc gctcaaagtg cttcggcagg tgttcctttc
     2761 cttttttgac agaaccaatc aaaataaagg gagaaacgat atgaaacatg actttcataa
     2821 aatcgactta aaattcggcg ccagagagtt gcgtcaactt ggagaacgtg aactctatta
     2881 tctccgtcag gttatggagg gagaaacttt aagcaactac atcagccctg atagtatgac
     2941 acgtcgtttt gaggaagcat tcgcaaaaaa gattggcgcc agaaaagcca tggcgaaaaa
     3001 cagtgccatg tctggtttat gtgaagcagt cagcgtatct ggcgcaggtc ctggttatga
     3061 agttatctgc gatccaattg tgcattttgg cggcctggca gcaatttatt ttaatgcggt
     3121 accacgtttt gctgatgtga aaagagatac ttataatatg gaccctgatt cattactggc
     3181 aaacattaca cctctcacaa aagcggtcat tgttacacat ttgtggggac aaagtgcaga
     3241 aattgatacc atcgctcaaa tatgccgtga gaaaggtata tttttaatag aggattgcgc
     3301 ccacgcaatc ggcgccacgt ggaacggaca acatgttggc acttttgggg acctgggcgt
     3361 attcagtttt caggaattca aacagctctc aacaggcgac ggcggtatga cagtcacaaa
     3421 taatgaagag ttgtttgacc gtatgcgcaa tgcatggtgg ttctccggcg aatctccaaa
     3481 attcatgtat cttaacttca gaatgaacga ggttaccgca gctgttggtc ttgcccagct
     3541 tgaaaaagtg gacaggatta ttgaaggtac ctataacaaa acactcgcaa tactgaataa
     3601 agccattgaa ggttgttcat ggctaaaacc ccgggccgta ccgaaacaag ctcatatggg
     3661 tggctactgg tgggcattta catgggaagg cgataaacag ggactatctt atgataaatt
     3721 caaacaatta aacgacgagt tagaaattgg attacgcttc ggcttcaatc agtatcccgc
     3781 ttatgagttt ccatttttcc gtcaatcaac tgcctacaga agccatccgg attgtcctgt
     3841 acgctgcccg ttttacacag ccagaagtaa ctatcgctac acatgggatt taacaccggt
     3901 atgcgccgat ttgatgcccc gtctcattac cgttaatcta attttcctat cagtgcctgc
     3961 ggctgaagaa atggcggaac ggattagaac ggctattaaa aaaatgaatg gttgaactat
     4021 tcaaaatttc aaggagaaaa catgaacatc ctgatttttg gtgcacatcc agacgatatt
     4081 gaaatactgt gcggtggtac gtcggctaaa tttaaacgtc tgggacacaa agtgacacac
     4141 tgtgttctaa ctgacggaca ggtctcatca cgacgaattc caaatcagga gctggtgcag
     4201 atacgaaaaa atgaagcaaa ggcagcagca gacgttattg gtgttgacct ggtgatgtgc
     4261 ggtataaaag acgaaatgct gtttgatgat gaagacaccc gattgaaggt gcttgatgtg
     4321 attttaactg tgcgcccaga tgttattatc accatgagtg ataaagatta cgcctcagat
     4381 cataaagcaa cgtacaatct tgtcattgct gtaattccta tgtcagttgt taaaaattgc
     4441 tattctcaaa aaccttgtct ggataagcat cccgttgtct acatgatgga tacaatatac
     4501 gggattgatt tccttcccac agagtatgtt gatatatcag aggattttga caccaaatta
     4561 aaggcgttgc gttgccatga gagtcaaatt gaaattggaa aagaattggg ttgcgattac
     4621 gaagaacagg ttaaggttgt ttcccgtttc cgcggtctgc aggcaggcgt gcgttatgct
     4681 gagggatttc agaaattgaa caactggtat tctggtgtta caacccgcta tttgccctga
     4741 ccaagcgttg cagaacgata ctgcgtttgc tggtaattac cttggactgc cgggtcaggt
     4801 cctgcaaaat cacactaccc ttcaaaatac aggaattggt ttgtgaaatt tcaggaagca
     4861 ttctataatg attgtcatgg aagaaccatt cattacaaaa gaaaaacttg tctctaacct
     4921 gaaaaacatc ggcgttgaag aaggagatgt cctttttatt cattcttcgt tcaaaagtat
     4981 tggaaaggta gaaggtggtg cccagacagt gattgatgct ctgaaagaat ctgttggaga
     5041 aaaaggcaca atattgatgc cagtattcaa tctcgtgaag gacagacctg gtacatggaa
     5101 tgtaacaaca accccatcta caacagggta tcttacagaa tatttcagaa ctctgccagg
     5161 cactgttcgt tcagaccact atagtcattc agtcgctgca tggggtgcgc atgcatatga
     5221 gtttgtttca ggccatcgag gcgcaaaagg tatgatttct ccatgggacc atgaacaatt
     5281 tggatgcacc tatggatacg aaagtccttt tatgaagctc tactgccaca ctaaaagcaa
     5341 aatcctgatg ctcggtgttg actaccattc atcaaccttc tgtcatcttg ttgaagtaat
     5401 tttctggaat gaaagattat tgacagacca aaaagcttcc tatgtctcga ttaacaggga
     5461 aaaactcgga caatattttg attcgctggg taaacttact cgttcattta tcgggcaggc
     5521 tgactcaaga cttttcaaaa taagagattt tgttgataca ctgtgtcgtg aagtaagaaa
     5581 gcatactgac aaatacgttt caatgcttat caacatgaag caatctatca gcacaggaga
     5641 agccatgaaa ataaaaatca taaaaaagag aatcatcagc aaagacaaca gttaccacgg
     5701 atggccaagt ttatgcaaaa gaaaaaatgg acaactgctt gttgtagcca gtggtggaag
     5761 gcaagcccat gtctgtccat tcggaaaaat atacctctac agaagtgatg acaatggaaa
     5821 tacatggtca gggccagaag tcctttataa tagtccgctt gatgatagag accccggtat
     5881 tcttgagacg gataacaaca ccctgcttgt aagctggttc acatcattga cctggatgaa
     5941 ttatttatac agggctgaga ttggcataat caaatggtta ccagaagaca cgtgtaaaaa
     6001 atggcgcgaa attcgggata aacttttgga agatgagata aatactggca gagaactgga
     6061 tacatggatg atcaggtcag aagatggcgg gaaaacgtgg tcagaaagaa taaaaatacc
     6121 tgtccattgt ccccatggtc cagtgcaatt gaaagatggt cgacttctgt ttgctggaag
     6181 acaatcgctt ccaccagaaa aacgttctct ttatggggca tcatttattg gtattgacag
     6241 ggaaaactca gaaattgcag ttgcagaatc aaacgatgat ggaaaaaact ggaaaataac
     6301 aggagttctg ccagtcttac cacctgtacc acctgataat ttttgtgagc ctcacctggt
     6361 tgaaacgaca tcaggaacac tgattatgca tatcagaaat caatgtccat ctgttttccc
     6421 cggagagata cttcaatctg aatctgaaga tggtgggaaa acatggagcg ttccgcatcc
     6481 tataggatta aagggattgc ctgctcactt actttgtctt cacaatggct ggattttaac
     6541 aacatatgga tacaggaaaa acccctttgg aatacgggca agtgtcagca aggatgaagg
     6601 aaaaacatgg tcagaaccaa ttgtgatcag cgatgatggg tataacagcg accttggtta
     6661 tccttcttca gtgcaaatag atgatgggag tatcatcact gtgtggtacg aggtgttaaa
     6721 ggacaatcca tttgcagtat tgaaaatggc tcactgggta tttatttaaa atcaaaagga
     6781 ggaaatcgtg cttaatcctg gcaaaaatgc tttgcttgcg cttgatacga tttataaaaa
     6841 accagcaaag ggcattccga gctgggtcat tcatcctatg gaacactctg tcatagatta
     6901 tcttgctggc gaagaaccag gtacttatgt taaaaatccc cgtcaaactt atctgaaaat
     6961 gcaaaaaaac gtaggaacat gtatgattga ccagtatatc cctgaaaacc ctttatcaat
     7021 tggatcaaga ggttacgaaa aaaagacaga aactccgaca acaggagcaa aggaaactat
     7081 ttatgatgga ataaaaattg attcgccaga ggcagtggtt gaacacatgg aaaaagttgt
     7141 gtcgccaaaa gtcaaacaca atattgaaac atggtcagaa gatttacttg ttcaagttgc
     7201 tgaaaaaata ataaataatg aacaggaaat tcaaaaagag tttggtcctg atatattaaa
     7261 agttccgtat ggagttgcga catttccagg ttttgcatac gggtactatg gttacgaaaa
     7321 ttatttcatg gcgtatgctt tatatcctga agtaatggaa aaatggttca gtttgcgtgc
     7381 tgattactgc cagttataca acaaggcagt tgcccttgcc tatgaaaagg caaacttacc
     7441 tcccctgttg cgacttgacc atgatatggc aggagaaaag ggtacactgg ttaatatcag
     7501 gtccattgat aaaatctggt ttccacattt ttaccactca gtcaaagaac tggctgacaa
     7561 aatcactttg atctggcatt gtgatggaaa tctgatggaa atggtcccaa ggttaataga
     7621 atgtggcatc aaaggatttc agggtttcca gtatgaatgc ggaatggatt atgaaaaaat
     7681 atgcaaaatg aaaacaagag atggagaaga cctttttatt attgctggtg tctctgtcac
     7741 aaggacatta ccatctggca ccac
//
LOCUS       DAORYU010000046         7724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_6759_length_7724_cov_4.264572, whole genome shotgun sequence.
ACCESSION   DAORYU010000046 DAORYU010000000
VERSION     DAORYU010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7724
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6759_length_7724_cov_4.264572"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="PLW07_02505"
     CDS             <1..341
                     /locus_tag="PLW07_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49425.1"
                     /translation="MFFIMFLIEEFRMRFAKNSGNIPALILFIWFFVVPLISSVYAYD
                     RHIKNFYLAWLSPTKFLIPVFLRDLSENTSLPCIITGLLLLVIATYLSFLFWKKSKRI
                     FEQKLFEIKN"
     gene            359..1705
                     /locus_tag="PLW07_02510"
     CDS             359..1705
                     /locus_tag="PLW07_02510"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOL49426.1"
                     /translation="MKADIIVIGAGPAGMAAAWAAAKNGADTLLIERYGFCGGMATTG
                     IIGSILGHYLNEKEPAVAGFLKALVEKTAELDGCEKWETAFKKYGISFDSEILKYAAE
                     QLLLDAGVRFLYHSYFVSSCVNEGNIKSIEIANKKGKMTVEGKYFIDATGDADIAYFS
                     GFECTKGRPQDGNTESIGCIFKIAGIDEEKLTDDVVKKAREQLAELRNSGELKIFNEG
                     IGGKGSTIRKGERSFNMTRFAGDGTNPVDLTEAEIFLRKETFKIFEFFKKNVKGFENS
                     YISAFHINAGIRETRQIKGLYTLTEQNVVEGIKTDDSIARCTYWIDIHCPFGRTIKTH
                     LCCKECQTPEYCVMLDKYKNYLPSRNKLYPPENDWFSIPVGCLIPEKSKNLAVAGRCI
                     SADHKAMGAIRVMATCMATGHAAGVLASIAGKKNCAISEVSATEVQEIITRQEGLY"
     gene            complement(1719..>3731)
                     /locus_tag="PLW07_02515"
     CDS             complement(1719..>3731)
                     /locus_tag="PLW07_02515"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOL49427.1"
                     /translation="AYAGGLVIRGHGLMKVTGTGYETAIGKIGKSLQSIEPARTLVQI
                     EIENLVKRFSISGLILCTIVVVSYRLLNYNWLNGILAGLTLAMAILPEEFPVVLTIFF
                     ALGAWRMSRHNVLTRKIPVIETLGAMTVLCVDKTGTITTNRMEVKGLYSEGEFLILTE
                     NSVSHLSENFHRLIEFGILASLERPFDPMEKAFRNFANRYLSGTEHIHENWTLVQEYY
                     LSDELFAMSNVWKSPDGNDYVIAVKGSPEAIVDLCHLTQTVQKEIESVVKKMAEQGLR
                     VLGVASARFVQGKVLPKNQHDFKFDFIGLVGLMDPPRENAENSIKLCKKAGIKVIMIT
                     GDYPATAVNIAKRIGLEPIHRIVTGQELLEMDDMTLMKNIENINVFARIMPEQKLRIV
                     DILKKKGEIVGMTGDGVNDAPALKSAHIGIAMGERGTDVARESADIVLLDDDISSIVK
                     GIETGRTIFDNLKKTVSYIISVHVPIIGMSLFPVLMGLPLVLGPVHIVFLEMIIDPAC
                     STVFEAEPSERGIMEKPPRKLNESLLDGRTFLFSILQGVVVFGVVFTIFLLALARGHD
                     EARSRAITFITLIISNICLILTNRSRVDTAFSKTLFSNRPLIYVVSGALIFLFFVIIN
                     PFLQNLFKFSYVDLHDVMFCVIAGFASILWFEGIKYFRRNSKKPML"
     assembly_gap    3732..3831
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3832..4043)
                     /locus_tag="PLW07_02520"
     CDS             complement(<3832..4043)
                     /locus_tag="PLW07_02520"
                     /inference="COORDINATES: protein motif:HMM:NF012893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HOL49428.1"
                     /translation="MSQLSGLTESEVQKLLKIHGYNEIPSSEHRTIVNILKDIMKEPM
                     FLLLVACGVIYLLLGNRLEAQMLLAFV"
     gene            complement(4068..5126)
                     /locus_tag="PLW07_02525"
     CDS             complement(4068..5126)
                     /locus_tag="PLW07_02525"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOL49429.1"
                     /translation="MAKQLDEKLLKKIRKGIVIPAHPLALTEKRSLDEKRQKALTRYY
                     LSAGAGGIAIGVHTTQFEIHDKKIGLYKPVLELASETITEFEEKHKPDTSVVRIAGVI
                     GKTQQAIEEATFAKDSGYHAVLLSLSAFRDSTNKDMIEHVHTISEIIPIFGFYLQPAV
                     GGRVLNYDFWREFAEIETVIAIKIAPFNRYQTIDVVRAIADSGRDIALYTGNDDNIVF
                     DLLTPFHFGNSTVRIVGGLLGHWACWTSKAVEILNRIHSVVEKGLPVSQDLLELGVEV
                     TDCNAAIFDAANNFSGCIPGIHEILRRQGLLKANWTLKEDENLSPGQKEEIDRIYTSY
                     PHLNDDKFVSDHLSEWLE"
     gene            complement(5129..6175)
                     /locus_tag="PLW07_02530"
     CDS             complement(5129..6175)
                     /locus_tag="PLW07_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOL49430.1"
                     /translation="MRDIKDIPDFIETEQHLDEFLSKPYYGVIEMCRALHGDIIILGV
                     AGKMGPSLALQIKRADEIAGIKRNIIGVARFSEPQIKKDLESSGIKTISCDLLDRNAV
                     DGLPDVKNVIFMAGMKFGSSVNQTMTWAMNACVPAFVAIKYKHSNIVALSTGNVYPLV
                     PVASGGSKETDTVGPIGEYAQSCLARERVFEYFSTKFSIPSVLIRLNYAVELRYGVIY
                     DIGVKVYKGEKINLENGHVNVVWQGWANSVVFRSFNICSIPPKILNLTGPEILSVRYI
                     AEEFGRLFSKKPLFEGKEADTAYLSDASECFRLFGRPDITPEKIIKWVARWIESGGRS
                     LNKPTHFEERKGRY"
     gene            6278..7183
                     /locus_tag="PLW07_02535"
     CDS             6278..7183
                     /locus_tag="PLW07_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOL49431.1"
                     /translation="MKFSHPEDEGIKTSSLIKRFLFIFISTLAILVGITTIIYISSAR
                     NELSLIENQELKNTSAISKLIVKSFDSSVSDILFLGAISSRYICEHGNEAKFLEEISS
                     FLVNKDYYTKITLIGNDGRILSQIVMEQKEPVRVQLNGKNYRDNPDYIKTSGIGRGRV
                     YVSDFQLDDGVPVLVFGTPVVDRQKQKKAVLLVNYNCKPVYDIFRITFEHQTEKNPAY
                     YIINDEGYWIKGPKHEDEFGFLFGNRNEKRFGYVYPEAWRKIAIDQTGQIFNREGLFT
                     FTTFYPIARAEKTSEQGNAELDNTS"
     gene            7331..>7724
                     /locus_tag="PLW07_02540"
     CDS             7331..>7724
                     /locus_tag="PLW07_02540"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOL49432.1"
                     /translation="MARITLKKEYAEQILEQLSVTDVLTDLYNRRGFFLLAEQQLRIA
                     ERIQEHMALFFMDVDDFKNINDIYGHQEGDRALQIIADTLEKTFRESDILGRIGGDEF
                     VALVIQGSPVDTDTILGRLQTNLDIVNQN"
BASE COUNT         2434 a         1553 c         1366 g         2271 t
ORIGIN      
        1 caatgttttt catcatgttt ctcatagaag aattcaggat gagatttgca aaaaactccg
       61 gtaatattcc agcactgata ttatttatat ggttttttgt cgtccctctg ataagctcgg
      121 tctatgcgta tgataggcac ataaagaatt tttatcttgc gtggctttcg ccaacaaaat
      181 ttttgatacc agtgtttctc agagaccttt cagaaaacac ttctctccct tgtatcatca
      241 ccgggttact tttgcttgtc attgccacat acctttcttt tctgttctgg aaaaaatcca
      301 agagaatctt tgaacaaaaa ctatttgaaa ttaagaattg aatgaaaaag ggggaattat
      361 gaaagcagat atcatagtta ttggggcagg accagcaggt atggctgctg cctgggcagc
      421 agcaaaaaat ggtgcagata cattacttat tgaaagatat ggattctgtg gtggaatggc
      481 aacaactgga attatcggtt caattctcgg tcattatctc aatgaaaaag aaccagcagt
      541 tgcagggttt ttgaaagcac ttgttgagaa gacggctgaa ttagatggat gtgaaaaatg
      601 ggaaaccgca ttcaaaaagt atgggatttc ttttgattca gaaattttga agtatgcagc
      661 cgaacaactt ctgctggatg ctggcgtgag atttctttat cactcgtatt ttgtctcgtc
      721 ctgcgtgaat gaaggaaaca tcaaaagcat tgaaattgcc aacaagaaag gcaaaatgac
      781 agtcgaagga aaatatttca ttgacgcaac cggagatgca gatattgcat atttttctgg
      841 ttttgagtgt accaaaggaa gaccacagga cggaaacact gaatcaattg gttgtatctt
      901 caaaattgct ggcattgacg aagaaaagtt gactgatgat gttgtaaaaa aagccagaga
      961 acaacttgct gaattaagaa acagtggcga attgaaaatt ttcaatgaag gcattggcgg
     1021 aaaaggaagc accatacgaa aaggagaaag aagtttcaat atgacgagat ttgcaggaga
     1081 tggaacaaat cccgttgact taacagaggc agagattttc ctcaggaaag aaacattcaa
     1141 aatttttgag tttttcaaga agaatgttaa gggatttgaa aactcataca tttcagcatt
     1201 tcacatcaat gctggcatta gagaaacaag acagataaag ggtttatata ccctgacaga
     1261 acaaaatgtt gttgaaggga taaaaactga tgatagcatt gcccgctgca cttactggat
     1321 tgacattcat tgtccctttg gaagaacaat aaaaacgcat ctgtgctgca aagaatgcca
     1381 aacaccagaa tattgtgtta tgcttgataa atataaaaat tatctccctt ccaggaataa
     1441 actgtatccg cctgagaacg actggtttag catacctgtt ggttgtttga ttcctgaaaa
     1501 atcgaaaaat cttgctgttg ctggcagatg tatttctgct gaccacaaag caatgggagc
     1561 aatcagggtc atggcaacgt gcatggcaac aggacatgca gcaggtgttc ttgcatcaat
     1621 agcaggcaag aaaaattgcg ctatctctga ggtcagtgca acagaggttc aggaaatcat
     1681 aaccagacag gaaggcctgt attaagacat ggatgatatt aaagcatggg ctttttggaa
     1741 tttctcctga agtattttat tccttcaaac cataaaatac tggcaaatcc tgcaatcaca
     1801 caaaacataa catcatgcaa atcaacataa ctgaatttga aaagattttg caggaatgga
     1861 ttgatgatta cgaaaaaaag aaaaattaac gctcctgaca ccacatagat aagaggtctg
     1921 tttgaaaaaa gggtttttga aaaagccgta tccactcttg aacgatttgt aagaattaaa
     1981 cagatgtttg aaatgattaa tgtaataaag gtaattgctc gcgaccttgc ctcatcatgt
     2041 ccacgagcca gcgccaataa aaaaatggtg aaaacaacac cgaaaaccac aacaccctga
     2101 agaatactga acaaaaaagt tctgccatca agaagtgatt cattaagttt tctgggcggt
     2161 ttttccataa tacctctttc tgaaggttct gcctcaaaaa ctgtggaaca tgcgggatct
     2221 ataatcattt cgagaaaaac aatatgcaca ggacccagaa caagaggaag tcccataagc
     2281 acagggaaaa gtgacatgcc tataatcgga acatgcacag aaataatata ggacacagtt
     2341 ttcttaagat tatcaaaaat cgttctccct gtttcaatac ctttaacaat ggaagaaata
     2401 tcatcatcaa ggagcacaat atccgctgat tctctcgcaa catctgtacc tctttctccc
     2461 attgcaatac caatatgcgc agatttaagg gctggagcat cattaacacc atcccctgtc
     2521 attccaacta tttcgccttt tttcttcaaa atatcaacaa tcctcaattt ttgctcgggc
     2581 ataatgcggg cgaaaacatt aatgttttct atgtttttca tcaaggtcat gtcgtccatt
     2641 tccagtagtt cctgcccggt tactattcta tgtattggtt caagtccaat cctctttgca
     2701 atattcactg ctgtagcagg ataatctcct gttatcataa ttactttgat gccagctttt
     2761 ttacagagtt ttatagaatt ttctgcattt tcccgtggcg gatccattaa acccaccagt
     2821 ccaataaagt caaatttaaa gtcgtgctga ttctttggca gtacttttcc ctgtacaaat
     2881 cttgcactgg caacaccgag caccctcaat ccctgttccg ccattttttt tacaacactt
     2941 tcaatttcct tctgtacagt ttgagtcagg tggcataaat ccacaatcgc ctcaggtgaa
     3001 cccttgacgg caatgacata gtcattccca tcaggagatt tccacacgtt tgacatcgca
     3061 aatagttcat cggaaaggta atactcctgc acaagtgtcc aattttcatg aatatgttca
     3121 gtaccagaaa gatatctgtt cgcaaaattt ctaaatgcct tctccatcgg atcaaaaggc
     3181 ctctcaagac ttgcaagtat tccaaattcg atcaaccgat ggaaattttc agatagatga
     3241 gaaacactgt tttctgtaag tatcaaaaat tctccttctg aatagagacc ttttacttcc
     3301 atacggtttg ttgtgatggt tcctgtttta tcaacacaca aaactgtcat tgcacccagg
     3361 gtttcaataa caggaatttt tctcgtaaga acgttatgac gagacattcg ccatgcacca
     3421 agcgcaaaaa atattgtaag aacaaccgga aactcttcag gaagaatcgc catcgcaagg
     3481 gtgagccctg caagaattcc atttaaccag ttataattaa gtaacctgta agaaacaaca
     3541 acaattgtac aaagaatcaa tcctgagatt gaaaatctct taaccagatt ttcaatctca
     3601 atctgaacaa gtgttcttgc aggttctatg ctctgcaagg acttaccaat tttcccgata
     3661 gctgtttcat atccggtacc tgtcactttc attaaaccgt gtcctcttat taccaatcca
     3721 cccgcatagg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nacaaaagcc
     3841 agaagcatct gggcttcaag tctattcccg agcaaaagat aaatgacccc acatgcaacc
     3901 aacagaagaa acataggttc tttcataata tccttaagga tattaacaat ggttcggtgc
     3961 tcagaagaag gtatttcgtt ataaccgtga atcttcaata atttttgaac ttcagattct
     4021 gttaaccctg aaagttgact cattgtcttc tctggtcata aataaaatta ttccaaccac
     4081 tcgctcagat ggtcagatac aaatttgtca tcgtttaaat gaggatagga cgtgtaaatt
     4141 ctgtctattt cttctttttg cccgggtgaa agattttcgt cttcttttaa tgtccagttt
     4201 gctttcaaca acccctgcct tctcaaaatt tcatggatgc ctggaataca ccctgaaaaa
     4261 ttgtttgcag catcaaaaat tgctgcatta caatccgtca cttctactcc aagttccaat
     4321 aaatcctgtg acaccggtaa tcctttctca acaacagagt gaattctgtt taatatctca
     4381 actgcttttg atgtccagca tgcccagtga cccaatagcc cacctactat cctcactgtg
     4441 ctatttccaa aatgaaatgg agtcaaaaga tcaaatacaa tgttatcatc attcccagta
     4501 tatagagcaa tatcccgacc actatcagca attgccctta ccacatcaat tgtttgatat
     4561 ctgttaaacg gtgcaatctt aatagcaata acagtttcaa tttcagcaaa ttccctccag
     4621 aaatcatagt tcagaaccct gcctccaaca gctggctgaa gataaaatcc aaaaattggt
     4681 ataatttcag aaattgtgtg cacatgttca atcatatcct tgtttgttga atccctgaat
     4741 gcagataaac tcagaagcac agcatgatat cctgaatcct ttgcaaaagt tgcttcttca
     4801 atcgcttgct gcgttttacc tatgacaccc gcaatcctga caactgaagt atctggcttg
     4861 tgtttttctt caaattcagt aattgtctct gaagcaagtt caagaaccgg tttgtaaagt
     4921 ccaattttct tatcgtgaat ttcaaactgt gtggtatgga caccaatcgc aattccacct
     4981 gcaccagccg aaagatagta tctcgtaagg gctttctgtc ttttttcatc aagagatctt
     5041 ttttctgtaa gtgccagtgg atgtgctgga ataacaatgc cttttctaat ttttttcaaa
     5101 agtttttcat caagttgctt cgccattttc aatacctccc ttttcgttcc tcaaaatgag
     5161 ttggtttgtt gagagaccta cccccgcttt ctatccagcg ggcaacccat ttaataattt
     5221 tttctggagt gatatctggt ctgccaaaaa gcctgaaaca ttcagaagca tctgaaagat
     5281 acgcagtatc tgcttctttt ccttcaaata acggtttttt tgaaaacaac ctaccaaatt
     5341 cctctgcaat gtacctcaca gaaagaattt caggacctgt tagatttaga atttttggcg
     5401 gaatagaaca aatattgaaa gacctgaaaa cgacactatt tgcccagccc tgccagacca
     5461 cattgacatg cccattttca agattgattt tttccccttt ataaaccttt acaccgatat
     5521 cataaatcac accatacctc aattccactg cataattgag ccgtatcaaa acagaaggta
     5581 ttgaaaactt tgtggaaaaa tattcaaaca ctctttccct ggcaaggcat gattgcgcat
     5641 actcgccaat cggacccacc gtatctgttt cctttgaacc ccctgacgcg accggcacca
     5701 atggatatac atttcccgtg gaaagcgcaa caatattaga gtgtttatac ttgatcgcaa
     5761 caaacgctgg aacacaggca ttcattgccc atgtcatggt ttgattaaca cttgaaccaa
     5821 atttcatccc tgccataaaa attacattct tcacatccgg aagaccgtcc actgcgttcc
     5881 tatcaagaag gtcgcatgaa atggttttaa taccagaact ttcaagatct tttttgatct
     5941 gtggttctga aaatcttgca actccaataa tattccgttt aatacctgct atttcgtctg
     6001 ctctttttat ctgtaaggca agagatggtc ccatcttgcc tgccactcca agtataataa
     6061 tatctccatg cagggctcta cacatttcta ttactccgta atatggctta gacaaaaatt
     6121 catcaagatg ctgctctgtt tctataaagt ccggtatatc tttaatatct ctcatcaatc
     6181 cccctttttc agcaattatt ttacaccaga acacgcctgt ttaaaatttg acaggatttc
     6241 aaatattcag taaattatta ttaacaagga tatgaaattg aaattctctc accccgaaga
     6301 cgaaggtatt aagacatcca gtttaataaa aagattcctc ttcatcttca tttcaacact
     6361 tgcaatactg gtcggcatca ccactatcat atacatctca agcgcgagaa atgaactctc
     6421 tcttattgaa aaccaggagc tgaaaaatac ttctgcaatc tctaaactga ttgtaaagag
     6481 ctttgatagt tcggtttcag acatcctttt tttgggagcg atttcttcca ggtatatttg
     6541 tgaacatgga aatgaagcca aatttcttga agaaatctct tcatttttag tcaataagga
     6601 ctattataca aaaataacac ttattggaaa cgatggcagg attctgtcac agatagtaat
     6661 ggagcaaaaa gaaccagtca gggtgcagtt gaatgggaaa aattataggg ataatcctga
     6721 ttatatcaaa acctctggaa ttggacgggg gcgtgtatat gtgtcagatt ttcaacttga
     6781 tgatggagtt cctgtccttg tgttcggtac gcctgttgtt gacagacaga agcaaaaaaa
     6841 agcagtactg cttgtaaatt ataattgcaa gccggtgtat gatattttca gaataacatt
     6901 tgaacatcaa acagaaaaaa atccagcata ttatataatc aatgatgaag gatattggat
     6961 aaaaggacca aaacacgaag atgaattcgg ttttcttttt ggtaatcgaa atgagaaaag
     7021 attcggctat gtttatccag aagcgtggag aaaaattgcg attgaccaga ctggccaaat
     7081 atttaacaga gagggccttt ttacttttac aacattttat ccgattgcac gagcagaaaa
     7141 aacaagcgaa caaggtaatg ccgaacttga taatacgtca taacatcaga taagaagttt
     7201 tgattacttc tggaaaatca tatcgtttgt accaaaacat ctatgtacaa ttcaatccac
     7261 tgcacgactt aaggaatttc ttctttatga cactgtactt gttcttttct ttatagttat
     7321 ttcgtggata atggcgcgta ttacgttgaa aaaggaatat gcggaacaaa tcctcgaaca
     7381 attgtcggtt acggatgtac taacagacct gtacaataga agaggtttct tccttcttgc
     7441 agaacaacaa ctgagaatag ctgaaagaat acaagagcat atggctcttt tcttcatgga
     7501 tgttgatgat tttaagaata tcaatgatat ttatggccat caagaaggag atagagcact
     7561 ccagataatt gctgatacac tggaaaaaac cttccgcgaa tctgatatcc tcggcagaat
     7621 tggtggggat gaatttgttg cccttgtcat tcagggttct cctgttgata cagataccat
     7681 actgggtcgg ttacaaacaa atcttgatat tgtcaatcaa aaca
//
LOCUS       DAORYU010000047         7717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_6768_length_7717_cov_4.652049, whole genome shotgun sequence.
ACCESSION   DAORYU010000047 DAORYU010000000
VERSION     DAORYU010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7717
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6768_length_7717_cov_4.652049"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PLW07_02545"
     CDS             <1..262
                     /locus_tag="PLW07_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49433.1"
                     /translation="KEGGNLVSLYKREMQEGFIEIHPDPPFSSFCFPPLRIPSVVSPF
                     IKGGLRGIFEISPSPSFIKRGVEWGLVKEGDLKLEGNFQNLP"
     gene            complement(367..798)
                     /locus_tag="PLW07_02550"
     CDS             complement(367..798)
                     /locus_tag="PLW07_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49434.1"
                     /translation="MISCTEFIPAYNELFKFLEKKGGKKAVIDFWNYISDNYLTNLNE
                     LMKKYGLKGCWLYWTKTLNEEAADFKMILDEKNNVFRIIMYKCPSKGRLMKIKHVKPY
                     RDYCEHCDVLYRRIIESFGYDYYLDLSKCDKAKCKFVIKKI"
     gene            complement(795..1259)
                     /locus_tag="PLW07_02555"
     CDS             complement(795..1259)
                     /locus_tag="PLW07_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49435.1"
                     /translation="MEKLVIRKKASDNVYFHPDFHIAMNTELKYLEGKYGYQAVKEYL
                     KQFARSYYKPLTEKIKKNGLKPLKEYIEQTYKKEGGKIKISFDKDSLKIKIAKCPAVA
                     YMRKRNVSIAKSFVETTKTVYETIVEKTPYSFEMCDYEAQTGKTTMIFRRKK"
     gene            complement(1266..2201)
                     /locus_tag="PLW07_02560"
     CDS             complement(1266..2201)
                     /locus_tag="PLW07_02560"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOL49436.1"
                     /translation="MKISKELHEKLCGVWSAAPTPFNKNMEIDREAVEKMVEHHIKIG
                     INGLFLLGTNGEGPCLTEKQKLEIVKQVVKCNKGKMVIAVQITDNSSQQMLENARKFS
                     DTGIDIFIISSPYFFVRPEPKRILNLYESVLKNIHLPVGIYDRGKHSAVEIPLSVLKK
                     LYTNKKIIIIKDSSSDETRMQLALMARRKNRNLRLFNGNEFQCVKYLQAGYDGLLLGG
                     GIFNGHIASQIINAVKTGNIGKAEKLQKLMNKIMYAAYGGKKVQSWLAGEKYLLMKMG
                     IFNTWNNLYQYTVDAQCEKTIDKIYKQYKNLLIPY"
     gene            complement(2203..3381)
                     /locus_tag="PLW07_02565"
     CDS             complement(2203..3381)
                     /locus_tag="PLW07_02565"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOL49437.1"
                     /translation="MSISVHAITPSFYKNACVSYNFTMINTIETGILYSNPKPHLKSV
                     HAYFPSVAQLPDGKMIAVYALGEAFEAINLHTHVSFSSDNGKTWQYAGRLLNEIKNKI
                     TSDASRITALPEGNIVILTCVYDRTGHTEEGLTNPDTVGFVPTSFYLIFSFDGGKTFS
                     APQQINPPVTGPCFELCCPVTILSDGTWLIPTSTWKAWDGSCPSGMKAIAFISTDKGK
                     NWGEYSVVMDAYSKGIIFWESKIIEMLDGNLVAVAWAYDGKNKIDLPNHFAISKDRGR
                     TWTEPISTDLYGQTLTSIVLKNGKLLSVYRRMDEPGLWAAISRIEGKRWITEQQIPLW
                     AAKVTGLTGNTKNPATDFHVLRFGAPSMIRLLDGSIFLAFWCYEQCISIIRWFLIREK
                     "
     gene            3364..4128
                     /locus_tag="PLW07_02570"
     CDS             3364..4128
                     /locus_tag="PLW07_02570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOL49438.1"
                     /translation="MYADTHCHLDFPDFDADREQVIKQAFDEKISLVINPGTDLPSSK
                     MAYEIAQKFNSVYAATGIHPHSANSVNDDIFQQCMELIEHDKVIAIGETGLDFYYQHS
                     KKSAQVELFKKHIKFAARFDIPLIVHQRDAEEEIIEIFKNETYPSKVVFHCFSGDEKM
                     LEWVMASGFYISFTGIITFRNATSVKKIAEKVNLDRVFFETDAPYLTPHPYRGKRNEP
                     RFVRFIVEEFARLRKLCAHEIAEITTANAIKFFGIR"
     gene            4134..5363
                     /locus_tag="PLW07_02575"
     CDS             4134..5363
                     /locus_tag="PLW07_02575"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HOL49439.1"
                     /translation="MKKIKIFTFGCKANQYDSQIFREVLLRQGYETVNTSCDIVLINT
                     CCVTKKAENEARKLVNKLLKNGKRVWVTGCWVEKDNLVSMFPEIKVVHREQLYQQAKI
                     YGIKGIGNFHKHTRAFVKVVDGCENFCTYCIVPYVRGNIKSRQLSEIVEEIKTLVHNG
                     FREIVLTGIDLGSYGRDNGSHLPELIQRISVIEGLTRFRLSSIEMFYIDGKLLNILSC
                     CKNFCPSFHIPLQSGSSKILKEMGRSYTYQQYKNRIDEIRSVFPFAGFSTDIIVGFPG
                     ETEQDFEMSINAIVECGFLRVHVFPFSPHPQTPASHLLEILPEKTKKERVLKTIEKAN
                     VISREVKGSFVDKKCIVLIEQKIHKFWTGYTESYVPVLVHSEENLCGSLIEMYPQSIM
                     KLNDTDYLYSDRYSILQ"
     gene            complement(5350..5922)
                     /gene="folE"
                     /locus_tag="PLW07_02580"
     CDS             complement(5350..5922)
                     /gene="folE"
                     /locus_tag="PLW07_02580"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017152519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOL49440.1"
                     /translation="MSRNLKEMEIEKSIKNIIKAIGDDPSRKGLKDTPKRVARMYCDI
                     FSGIHTNPEEILGKVFHEQYNELVLLKEIPLYSMCEHHLLPFFGHAHIAYLPDGKRIV
                     GISKLARLVDVYAKRPQVQERLTNQIVDTIMKVLQPQGAMVVIEAEHMCMIMRGVKKP
                     GTKIVTSAMRGIFLRDIRTRSEALNLIIAK"
     gene            complement(5891..7675)
                     /gene="ftsH"
                     /locus_tag="PLW07_02585"
     CDS             complement(5891..7675)
                     /gene="ftsH"
                     /locus_tag="PLW07_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOL49441.1"
                     /translation="MWLLLGVLLFLVVRTATMISENRKNLNYSDFIEEVKNGNVQGTL
                     LVKRGYVYGNLKDGTKFQTYAGEDPDLIKILRENNTSFKVEPASDFWAGLFINLIPVI
                     LIVGFFLFLINRQATNESNKVFSFSRSRPTMPDSKNRITFADVAGSEEAKEELKEIIE
                     FLKDPKKFQRLGGKIPKGVLLIGPPGTGKTLLAKAVAGEAGVPFLGMSGSDFVEMFVG
                     VGAARVRDLFRQAKRLAPSIVFIDEIDAVGRQRFAGLGGGHDEREQTLNQLLVEMDGF
                     VTDQGIIVMAATNRPDVLDPALTRPGRFDRTVVVGLPDIREREAILKVHTRNKPLAPS
                     VDLSVIARSTPGLSGADLANIANEAALLAARRNKNYIDMEEFEAAKDKILMGVERKSL
                     VISEEEKKVIAYHEAGHTIVQLSLPELHPVHKVTIIPHGQALGITHILPEKDKFIESD
                     TYFRNTLASLLAGRITEKIVFGKMFTGSENDLRIATDIARKMVCEWGMSEKLGPVSFK
                     EHEAVFLGRDLVQQRTISEDTNKEIDNEIKLILENASHTAYEIITKNRDKLDLLVKEL
                     LEHETLNSEQIRKILGGEHEQEPERDGN"
BASE COUNT         2210 a         1461 c         1427 g         2619 t
ORIGIN      
        1 taaagagggg ggtaacttgg tctcccttta taaaagggag atgcaagagg gatttattga
       61 aatccatccc gaccctcctt tttcctcctt ctgtttcccc cctttacgaa ttccttccgt
      121 cgtttccccc tttataaaag ggggattgag ggggattttt gaaatctccc ccagcccctc
      181 ttttataaag aggggggttg aatggggatt ggtaaaggaa ggagacctga agttagaggg
      241 aaattttcaa aatctcccct aacccctttt ttataaagag gggagaaaat tggaattaat
      301 ggagtaaaag tttaaagtgg agagaaactc ttcaacaaaa acgaactgtg aataaattcg
      361 tgaatttcat attttcttta ttacaaattt gcattttgct ttatcacatt ttgaaaggtc
      421 taaataataa tcataaccga acgattcaat gattcttcta tacagaacat cgcagtgctc
      481 gcagtaatcg cggtatggtt ttacatgttt tattttcatt aatcttccct ttgatggaca
      541 tttgtacatg ataattctaa acacattatt tttttcatca agtatcatct tgaaatctgc
      601 tgcttcttca tttaatgttt ttgtccagta cagccagcaa cccttaagcc cgtatttttt
      661 cataagttcg tttagatttg tgagataatt atcagaaata taattccaga aatcaatcac
      721 tgctttttta ccgccttttt tttcaagaaa cttaaatagt tcattgtacg cagggataaa
      781 ttcagtacat gatatcactt ttttctcctg aaaatcatgg ttgtttttcc tgtttgtgct
      841 tcataatcac acatttcgaa tgagtatgga gttttctcaa caattgtttc gtatactgtt
      901 tttgttgttt ctacaaatga ttttgcaata gaaacgtttc tttttctcat atatgcaacc
      961 gcaggacatt ttgctatttt tattttcaat gaatctttgt caaaagaaat tttaattttt
     1021 ccaccttctt ttttatatgt ttgctcgatg tattccttca atggtttaag cccattcttt
     1081 tttatttttt ctgttaaggg tttatagtac gaacgggcaa attgtttcaa atattctttg
     1141 actgcctgat aaccatattt tccttcaaga tatttcagtt ctgtgttcat cgcaatatga
     1201 aaatcaggat gaaaatagac attgtctgat gctttttttc ttatgacaag tttttccatg
     1261 tatttttaat acggaatcaa taaatttttg tactgcttgt aaattttatc aatggttttc
     1321 tcacattgtg cgtctactgt atattgataa aggttgttcc atgtgttaaa tatgcccatt
     1381 ttcattaaga ggtatttttc tccagcaagc caggattgaa cttttttccc gccatacgca
     1441 gcatacatga ttttattcat caatttctgt agtttttctg ccttccctat atttccagtt
     1501 ttcactgcat tgatgatttg agaagcaatg tgaccattaa aaatcccgcc gccaagaagg
     1561 agtccatcat atcctgcctg cagatactta acgcactgaa attcattgcc attgaaaagt
     1621 cgtagatttc tgttctttct tcttgccatt agagcaagct gcattctggt ttcatcgctt
     1681 gaactgtctt taatgataat aatttttttg tttgtataaa gttttttcag gacactgaga
     1741 ggaatttcaa cagccgagtg ttttcctcta tcatagattc caaccggtaa atgtatattt
     1801 ttcaaaacac tctcataaag attgagaatt ctttttggtt ctggcctgac gaaaaagtaa
     1861 ggtgaactga tgataaagat atcaatacct gtgtctgaaa attttcttgc attttccagc
     1921 atttgctgac ttgagttatc tgttatctga accgcaatta ccatcttccc tttattgcat
     1981 tttacaacct gttttacaat ttcaagtttt tgtttttcag taagacaggg tccttcaccg
     2041 tttgtaccaa gcaagaaaag accattgatt ccgattttga tgtgatgctc gaccattttt
     2101 tcaactgctt cgcgatcaat ttccatattt ttattgaacg gtgttggagc agcagaccac
     2161 acaccgcata atttctcatg aagttctttt gaaattttca tatcatttct ccctaatcaa
     2221 aaaccaccga ataattgaga tgcactgttc ataacaccag aaagcaagga aaattgaacc
     2281 atcaagtaat cgtatcattg atggagcgcc aaacctgagt acatgaaaat ctgttgcagg
     2341 attttttgta ttgcccgtta atcctgtaac ttttgctgcc cataacggga tttgctgttc
     2401 tgtaatccag cgctttcctt caatgcgtga aatggctgcc cacagtcctg gttcatccat
     2461 tcttctataa acgctcagca gtttcccgtt tttgagcaca atagaggtaa gcgtttggcc
     2521 gtaaaggtca gtggagatgg gttctgtcca tgttctgcct ctgtctttac tgattgcaaa
     2581 atgattggga agatcaattt tgttttttcc atcgtacgcc catgcgacag cgaccaggtt
     2641 tccatccagc atctcaatga tttttgattc ccagaaaata atacccttgc tgtaagcgtc
     2701 catgacaact gaatattcgc cccagttttt acctttatcc gttgaaatga aagcaattgc
     2761 cttcattcct gatggacaac taccatccca tgctttccag gttgatgtgg gtatgagcca
     2821 tgtgccatca gaaagaatcg tgacaggaca gcataattca aaacatggtc cagtaacagg
     2881 tgggtttatt tgttggggag cagaaaatgt ctttccacca tcaaaagaaa aaattagata
     2941 gaaacttgtt ggtacaaacc cgaccgtatc aggattcgtc aatccttctt ctgtatgtcc
     3001 tgttcgatcg tatacacacg ttaaaatgac aatgttgcct tctggtagag ctgttattct
     3061 tgaagcatct gatgtaattt tattttttat ttcattgagt aatcttcctg catattgcca
     3121 ggttttgccg ttgtcggatg agaaagaaac atgggtatgc agattaattg cttcaaatgc
     3181 ttcgccgagc gcataaactg caatcatttt accgtcaggc aattgtgcta cagatgggaa
     3241 ataagcatgc acacttttca ggtgtggctt tggatttgaa tataatatac ctgtttcaat
     3301 agtgtttatc atggtaaaat tataagatac acaagcattt ttgtaaaaag aaggagttat
     3361 agcatgtacg ctgatactca ttgtcatctt gattttccag attttgatgc agaccgtgag
     3421 caggtaataa aacaggcatt tgatgaaaaa atatctctgg ttatcaatcc agggactgat
     3481 ttaccatcaa gtaagatggc ttatgagatt gctcagaaat ttaattctgt atatgcagca
     3541 acagggattc atccgcacag cgctaattct gtcaacgatg atatttttca acagtgtatg
     3601 gaacttattg aacatgataa ggttatcgcc attggtgaaa caggtcttga tttttattat
     3661 cagcattcaa agaaaagtgc acaggttgag ttattcaaga aacatattaa atttgctgca
     3721 aggtttgaca ttccattgat tgttcatcaa cgagacgcag aagaagaaat tatagaaatt
     3781 tttaaaaatg aaacctatcc ttcaaaggtt gtgtttcact gtttcagtgg ggacgaaaaa
     3841 atgcttgaat gggttatggc gtctggattt tatatttcct ttactggcat tattacgttt
     3901 cgcaatgcca ccagtgtaaa gaaaatagca gaaaaagtta atcttgacag ggtatttttt
     3961 gaaacagatg ctccgtatct tacaccgcat ccgtacaggg ggaaaagaaa tgaaccgcgt
     4021 ttcgtcagat ttattgttga agaattcgca agattgagaa aactttgtgc tcatgaaatc
     4081 gcagaaatta caacagcaaa tgccataaaa ttttttggta tcagatagtt gatatgaaaa
     4141 aaataaaaat cttcacattt ggttgtaaag cgaaccagta cgacagtcag attttcaggg
     4201 aagtgttatt aaggcagggt tatgaaactg ttaacacatc ctgtgatatt gttttaatca
     4261 atacctgctg cgttacaaaa aaggcagaga atgaggcaag aaaattggtc aataaactat
     4321 tgaaaaatgg gaaaagggtc tgggtcacag gatgctgggt tgaaaaggat aatctggttt
     4381 caatgtttcc tgaaattaaa gtagttcaca gggaacaatt ataccagcag gcaaaaatct
     4441 atgggataaa aggcattgga aatttccata aacatacccg tgcttttgta aaggttgttg
     4501 acggatgtga aaacttttgt acctactgca ttgttcctta tgtcagagga aacattaaaa
     4561 gcaggcaatt atctgaaatt gttgaagaaa ttaaaactct ggtgcataat gggtttcgtg
     4621 aaattgttct tacaggaata gaccttggaa gttatgggag agacaatggt tcgcatctgc
     4681 cagaattgat acagagaatc agtgttattg aaggactgac aagattcagg ttgagttcaa
     4741 ttgaaatgtt ttatattgat ggtaaacttt tgaacattct gtcgtgttgt aaaaacttct
     4801 gcccctcttt tcatattccg cttcaaagcg gttcttcaaa aattcttaag gaaatggggc
     4861 gatcatatac atatcagcag tataaaaacc gcattgatga aattcggtcg gtttttccgt
     4921 ttgctggctt cagtactgat atcatagtcg ggttccctgg agaaacagaa caggattttg
     4981 aaatgagtat taatgccatt gttgaatgtg gatttttgag ggttcatgtg tttcctttca
     5041 gtccgcatcc ccaaacacct gcatctcatt tactggaaat acttcctgaa aaaacgaaaa
     5101 aggaaagggt tttaaaaact attgagaaag caaacgtgat ttcacgtgaa gtgaaaggtt
     5161 catttgtaga taagaagtgt attgtgctta ttgagcaaaa gatacacaaa ttctggactg
     5221 gttatactga atcttatgtt cctgtgcttg ttcattcaga agaaaatctt tgtggttcgc
     5281 ttattgaaat gtacccgcaa tcaatcatga aactgaacga cacagattat ctttattctg
     5341 acagatattc tattttgcaa taataagatt cagggcttca gagcgtgttc ttatatctct
     5401 taggaaaatt ccgcgcattg cggatgtaac aatttttgtg ccaggttttt ttactcccct
     5461 cataatcatg cacatatgct ctgcttcaat cacgaccatt gcgccctgtg gttgcagtac
     5521 cttcataatt gtatcaacaa tctgatttgt caatctttcc tgaacctgtg gcctttttgc
     5581 atagacatca accaatcgtg cgagtttgct gataccaacg attcttttgc catcaggaag
     5641 atatgcaata tgggcatgtc caaaaaatgg aagtaaatga tgttcgcaca ttgaatatag
     5701 gggtatttcc ttgagcagga ccaattcatt atattgttca tgaaaaacct ttcccagaat
     5761 ttcttcagga ttggtatgaa taccagaaaa aatgtcacaa tacattcttg cgactctttt
     5821 gggggtatct ttcaaaccct ttcttgaagg atcgtcacca atggctttaa tgatgttttt
     5881 aatagatttt tcaatttcca tctctttcag gttcctgctc atgttcgccc ccaaggattt
     5941 ttcgtatctg ttcagaattc aatgtttcat gttcaagaag ttcttttaca agcaaatcaa
     6001 gtttgtcgcg atttttcgta ataatttcat acgcggtgtg cgacgcgttt tcaagtatta
     6061 gttttatttc attgtctatt tctttatttg tatcttcgct tattgttctt tgttgaacaa
     6121 gatctctgcc caaaaaaaca gcctcatgtt ctttgaaact gactggtccg agtttttcac
     6181 tcattcccca ttcacatacc atttttcttg ctatatctgt tgctatgcgc aggtcatttt
     6241 cacttcctgt aaacatcttt ccaaacacaa ttttttctgt aatacgacct gcgagcaggg
     6301 atgcaagggt atttctaaaa tatgtgtcac tttcaatgaa tttatctttt tcaggaagaa
     6361 tatgagtaat tccaagcgcc tgtccatgag gaataatggt taccttgtga actggatgta
     6421 gttctggtaa tgagagttga acgattgtat gacctgcttc atggtatgca atgaccttct
     6481 tttcttcttc gctgataacc agactttttc tttcaacacc cataagaatt ttgtcttttg
     6541 ctgcttcaaa ttcttccatg tcaatgtaat ttttatttct tctggctgca agtaatgccg
     6601 cttcatttgc gatgtttgct aaatctgcgc cagaaagccc tggagtgctt cgcgcaatta
     6661 ctgaaaggtc aactgatgga gcaaggggct tatttcttgt gtggactttc aatattgctt
     6721 ccctttctct gatatccgga agtcctacta caacggttct gtcaaacctt cccggtcgtg
     6781 tgagagcagg gtccagaaca tcaggtctgt tggtcgctgc catcacaata attccctggt
     6841 ctgtaacaaa tccgtccatt tcaacaagaa gttgattgag tgtttgttct ctttcgtcgt
     6901 gaccaccacc aagtccagca aatctctgcc tgccaacagc atcaatttca tcaataaaaa
     6961 cgatggaagg agcaagtcgt tttgcctgcc tgaacaggtc tctaaccctt gccgctccaa
     7021 caccgacaaa catttctaca aaatcagaac cactcattcc aagaaaaggt acaccggctt
     7081 ctcctgcaac tgcctttgca agaagagttt tgcctgtgcc agggggtcct atcaggagta
     7141 caccttttgg aattttgcca ccgagtcgct gaaatttctt cggatcctta agaaattcaa
     7201 taatttcttt tagttcttcc tttgcttctt cagaaccagc aacatctgca aatgtaattc
     7261 tatttttgct gtcaggcatc gtcggtctgc tgcggctgaa agaaaacacc ttattgcttt
     7321 catttgttgc ctgcctattt attaagaaaa ggaaaaaacc aacaatcaaa attacaggaa
     7381 tcaaattgat aaaaaggcca gcccagaaat cagaggcggg ctcaacctta aaggatgtgt
     7441 tgttttcgcg caaaattttg attaggtcag ggtcttcacc tgcatatgtc tggaattttg
     7501 ttccatcttt taaatttcca tatacatagc cgcgtttaac cagaagtgta ccctgaacat
     7561 tgccattttt aacttcttca atgaagtcgc tatagtttaa attttttcta ttttctgaaa
     7621 tcatagtggc tgttctgacc acgagaaaca aaagcacacc taatagtagc cacattacaa
     7681 tgccttttac aaacgattct ttcggtaatt caggttt
//
LOCUS       DAORYU010000048         7605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_6875_length_7605_cov_4.467815, whole genome shotgun sequence.
ACCESSION   DAORYU010000048 DAORYU010000000
VERSION     DAORYU010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7605
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6875_length_7605_cov_4.467815"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..86
                     /locus_tag="PLW07_02590"
     tRNA            14..86
                     /locus_tag="PLW07_02590"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:47..49,aa:Trp,seq:cca)
     gene            105..287
                     /gene="secE"
                     /locus_tag="PLW07_02595"
     CDS             105..287
                     /gene="secE"
                     /locus_tag="PLW07_02595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOL49442.1"
                     /translation="MQKIKMFLKESQVEFKKVTWPNFQVLKRSTFAVIVLMILMAIYF
                     GIIDVIFSRMLHVFAR"
     gene            290..820
                     /gene="nusG"
                     /locus_tag="PLW07_02600"
     CDS             290..820
                     /gene="nusG"
                     /locus_tag="PLW07_02600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOL49443.1"
                     /translation="MSKWFIVHVRTGHEEKIKKLIESRLVNTGNANIKQIIIPTEGVI
                     DSTKGEKTVKPRKFFPGYLLIEAEDDVDEMTWHLVKTTNGVFNVLGAGTRPAPLQNKE
                     ITNLLQEIEERKTKPVPKVEFDKGDKVVITDGPFLDFTGVVEEVNNERERLKVSVSIF
                     GRSTILELNFWQVEKV"
     gene            823..1260
                     /gene="rplK"
                     /locus_tag="PLW07_02605"
     CDS             823..1260
                     /gene="rplK"
                     /locus_tag="PLW07_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOL49444.1"
                     /translation="MAEKSSKPIKAVVKLQIPAGQATPAPPVGPALGQHGVNIMDFCK
                     SFNAATKGKEGDIIPVVLTIYEDRSFKYQLKTPPTAALLKKVANIVKGSGVPNKEKVG
                     KVTRKQVEEIAKIKLPDLNTEDLAQAVKIVEGCARSMGIDIID"
     gene            1270..1986
                     /gene="rplA"
                     /locus_tag="PLW07_02610"
     CDS             1270..1986
                     /gene="rplA"
                     /locus_tag="PLW07_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOL49445.1"
                     /translation="MKRRSKKYKAIQERLEKKAVYTPEEAIAFIKANPSSRFDETVDL
                     AVHLGLDLKKLQQPVRGSVNLPHGSGKQVKVLVFAQGENIEKARNAGADYFGGNELVE
                     KIKSGWLDFEAVVATPDMMRNIAALGKILGPRGLMPNPKSGTVTFEIEKIVEELKKGR
                     IEFKMDKDGNLHIPIGKVSFSQEQLLDNLYAALKTIVAAKPQGAKGIYLKSAHLSSTM
                     GPSFPINISKISQEIKSTIS"
     gene            2004..2531
                     /gene="rplJ"
                     /locus_tag="PLW07_02615"
     CDS             2004..2531
                     /gene="rplJ"
                     /locus_tag="PLW07_02615"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOL49446.1"
                     /translation="MNKNDKAKLLKFLSDEIETSKVSILAEFSKLTVKEMEELRKEMK
                     KYSTKVMVVKNTLMRKAFQSFALDEAMNFMEGPNVLVWSKTADESDVVRELLKYSKIS
                     GKIKVKFGILNKSFIGVDTIERIGTLPSKKTLQAMVIGGIRAPLNGLVYNIKYPVTRL
                     ILVLKTFSEKKEKEK"
     gene            2528..2920
                     /gene="rplL"
                     /locus_tag="PLW07_02620"
     CDS             2528..2920
                     /gene="rplL"
                     /locus_tag="PLW07_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOL49447.1"
                     /translation="MSEKNLSVDALVEAIGSLTAMELADLAKKLEDKFGVQPVMATAA
                     VAPVASAEQAAGPVEEKTSFDVILTGFGANKIQVIKEVRAITNLGLKEAKDLVESLPK
                     PLKEKVSKEEADEIKKKLEAAGASVEIK"
     gene            2969..>7605
                     /gene="rpoB"
                     /locus_tag="PLW07_02625"
     CDS             2969..>7605
                     /gene="rpoB"
                     /locus_tag="PLW07_02625"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF001616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOL49448.1"
                     /translation="METKHLQIPNLLKVQTEPYNKFLQKDIPPEKRERIGLEEILRKV
                     FVETDLFPGEKHHVIASDDGTLFLEYISYAIEDPIESELECVEKGITYGGKFKVKFRL
                     YKKDKIHNRIESVREAQDVYLGLMPLMTERGTFIINGIERTVVNQIQRSPGVYFKEEE
                     EIGQRPTFSARIFPARGLWMEFHPELHHNITCLYAYFGKRKVYATTLLKALGYSPFEI
                     PSLFYEAPDRVPNDSIIKTTLEKDSDIKTPEDAIRKIYLELRPGFPMEEREAKSFFKR
                     LFFTSEQYDLSPAGRMQINKKLGLNIKEQHLTVDDIIKTIKYLLNLMEKNQGETDDID
                     HLGNKRVRTSAELVKEHVYEGLVRLAHFVKERMAIIDKAQDISPQDILNSRIFSTVVE
                     EFFARNQLSQFLDQVNPLSELTNKRRVTAVGEGGVKKRKRAGFEVRDVHYTHFGRVCP
                     IETPEGANVGLINSLTVYAEVDGMGFIRTPYYRVKNGRILFNEIVRVSADEEDNYVVA
                     PPDVPFDPKTGAIIPETIRVRSKGGEFTEASKDQIELIGVSPKQVVSLSASLVPFLEH
                     NDSNRALMGSNMQRQAVPLLRTESPLVRTGMEKKVVMDSGVVVKAINSGVVSYVDARK
                     IIIERDEKGIFPWQHHDVYYLQTFRRTNQDTCFHQKPVVSPGQKVKKGQMISDGPAVS
                     LEGDLALGRNLLVAFMPWRGYNFEDAIVLSERLVKEDTFTSIHIERFEVTAQETERGP
                     EEITADLPNVSEEELRNLDKNGIVRIGAEVKQGDILVGKITPKGEAELTPEEKLLRVI
                     FGEKARDVANTSLTVPPGIHGVVVNVKTYYPSDRIPEEDIKKRIDEVEKELKRKKNIV
                     RKIITDQIKNILSSHRISLARLGTSSESWDKIVNSIDRDNVKDEVRRIVEIGKKEIEK
                     LEHDAKNEEIKIRRGSELEPNVICKVSVEIAIKKKVAVGDKMSGRHGNKGVISVVLPE
                     EDMPFLEDGTPVDIVLNPLGVPSRMNIGQILETHLGWALKTLGYSMETPVFESLKEME
                     ITQKLREAGLPEDGKTMLYDGQNGEPFLQPVTVGYIYMMKLIHLADEKFHARSVGSYS
                     LITQQPLGGRAQFGGQRFGEMEVWALEGYGAGHMLREILTIKSDDIKGRKKAYESIVK
                     GLDYQEENLPESFHVLTKELNGLGFDLRIERKRKSDEEKDVVTIRIAPPMVIRSWSYG
                     EIKKAETLNYRTNKPERDGLFCEKIFGPVKDWECACGKFKKQRYRGVICDRCGVEVTT
                     SDVRRERMGHIELATPVSYVWLFKSSANWLGVLLDLTQMTLEKVLYYDRYIVVDPGDT
                     GLKEKELLTEDEYRENLGKYGNKFRAEIGASAIYELLKRIDLEKEMSKVKEQLKAKKT
                     KKDHTGRRNLVKKLRMIEGLIKTEVKPEYLVTSIIPVIPPDLRPLLPLDGGKFVASDL
                     NDLYQRVINRNNRLKKLIKLQAPDIIIRNEKRMLQEAVDALLDNGRYGTPVLGKGKRP
                     LKSLSESLRGKQGRFRQNLLGKRVDYSGRAVIVVDPKLKLHECGIPKQMAL"
BASE COUNT         2537 a         1227 c         1822 g         2019 t
ORIGIN      
        1 agagaaatca gataggcctg tagctctaac ggcagagcgg cggtctccaa aaccgaaggt
       61 tggaggttca aatcctccca ggcctgtaac aatggtgaaa atttatgcaa aagataaaga
      121 tgtttttgaa agaaagtcag gttgaattca aaaaagtgac ctggccaaat tttcaggtgc
      181 ttaaaaggtc aaccttcgcg gtgattgttc tgatgatttt aatggcaatt tatttcggta
      241 tcattgatgt aattttttca agaatgcttc atgtgtttgc gaggtaataa tgagtaaatg
      301 gtttattgtt cacgttagaa ccggacatga ggaaaagatt aaaaaactga ttgaatcacg
      361 acttgtgaat actggcaatg ccaatataaa acaaattatt attccgacag agggcgtgat
      421 agatagtaca aagggtgaaa aaaccgtgaa accaagaaag ttttttccgg gttatttgct
      481 catagaggca gaagacgatg ttgatgaaat gacatggcat cttgtgaaaa caaccaatgg
      541 agtatttaat gtgctcggcg cagggacaag acccgcacct ctacagaata aggaaattac
      601 caatcttcta caggaaattg aagagagaaa gaccaaaccg gttccaaaag tggaatttga
      661 taaaggggat aaggttgtga taaccgatgg gccatttctt gattttacag gcgtggttga
      721 agaagttaat aacgaaagag aaagattgaa ggttagtgtt tcaatttttg gaaggtcaac
      781 aattttagaa cttaatttct ggcaggttga aaaggtgtga atatggcaga aaaatcatca
      841 aaaccgatca aggcagttgt gaaattgcag attccagcag gtcaggctac tcctgctcca
      901 cctgtaggac cagcactagg gcaacatggg gtcaacatca tggatttctg taaatccttt
      961 aatgctgcaa caaaggggaa agaaggcgat attattcctg tggttcttac catttatgaa
     1021 gacaggtctt ttaaatatca attaaaaacc ccgcccaccg cagcgctgtt aaaaaaggta
     1081 gccaacattg tgaagggttc tggagtgccc aacaaggaaa aggtgggcaa ggttacaaga
     1141 aaacaggttg aggagatagc gaaaataaaa ttaccagacc ttaatacaga agatcttgcg
     1201 caggcagtga aaatcgttga gggttgtgca agaagtatgg gaatagatat tatcgattga
     1261 ggggtaaaga tgaaaagacg atccaagaaa tacaaagcaa ttcaggaaag gttggaaaag
     1321 aaagctgttt atacaccgga agaagccatt gctttcataa aagccaatcc ttcatctcgt
     1381 tttgatgaaa cggttgacct tgctgttcac cttgggcttg acctgaagaa gcttcagcaa
     1441 cctgtaaggg gttctgtgaa tcttcctcat gggagtggaa aacaggtaaa ggttctggtt
     1501 ttcgctcagg gtgaaaatat tgaaaaggcg cgcaatgcgg gtgcagatta ttttggcgga
     1561 aatgaacttg tggaaaaaat taagagtgga tggctggact ttgaggcagt cgttgctacg
     1621 cctgatatga tgagaaatat agctgcttta ggaaaaatcc ttggtccaag aggtctgatg
     1681 ccaaacccga aatccggtac tgtgacattt gagatagaga agattgtgga ggaattgaaa
     1741 aaaggaagga ttgagttcaa aatggacaag gatggaaacc tgcatattcc tattggaaag
     1801 gtttcatttt ctcaggaaca gcttcttgat aatttgtacg ctgccctcaa aacgattgtt
     1861 gcggctaagc cacagggcgc aaagggtatt tatttaaaaa gtgcccacct ttcttccaca
     1921 atggggccat cttttccaat aaatatcagt aaaatttcac aggaaatcaa gtcgactatt
     1981 tcctgaaata acaaggtgaa cgtatgaaca aaaacgacaa ggccaaactt ttgaaatttt
     2041 tgtcagacga aatagagaca agcaaggtct caatccttgc agagttttca aaacttactg
     2101 ttaaagaaat ggaagaacta cgcaaagaga tgaagaagta ttcaacaaag gtgatggttg
     2161 tgaaaaatac cttgatgagg aaagcatttc agtcttttgc acttgatgaa gccatgaatt
     2221 ttatggaagg gccgaatgtc cttgtctgga gtaaaacagc agatgaatcg gatgttgttc
     2281 gagaactttt aaaatattca aaaatttcag gaaaaattaa ggtaaaattc gggatcttaa
     2341 ataaatcatt tataggggtt gatactattg aaagaattgg cacgcttcct tcaaagaaaa
     2401 ccctgcaggc aatggtcatt ggaggtatcc gtgctccttt aaatggtctt gtatataaca
     2461 ttaaatatcc agtaacacgg ttaattcttg tgttaaaaac gttttctgaa aaaaaggaga
     2521 aggaaaaatg agtgagaaaa atctttctgt ggatgcgttg gttgaagcaa ttggaagtct
     2581 gactgccatg gaacttgctg acctggcaaa aaaacttgaa gacaagtttg gagttcaacc
     2641 tgttatggcg acagcagccg ttgcaccggt tgccagtgct gaacaggcag ctggtccggt
     2701 tgaagaaaag acatcttttg atgtcattct gaccggtttt ggagcaaaca agatacaggt
     2761 tattaaagag gtcagggcta tcacaaatct tggtttgaaa gaagccaaag atcttgtgga
     2821 atctcttcct aaaccattaa aggaaaaggt gagtaaagaa gaagcagatg aaattaagaa
     2881 gaagcttgaa gctgccggtg cgtctgttga aataaaataa aaaaaggttg ttaatgttag
     2941 agaaccctta acaggggtaa tggagaatat ggaaacgaaa catttgcaaa ttccaaattt
     3001 gctaaaagtt caaacagaac cctataataa gtttcttcag aaagatattc ctccagaaaa
     3061 gagggaaaga attgggcttg aagaaatact tagaaaagtt tttgttgaga cagacctgtt
     3121 tcctggtgaa aagcatcacg tgatagccag tgacgatggt actctgtttc tcgagtacat
     3181 tagctacgca atagaagacc cgattgaatc tgaacttgaa tgcgttgaaa aggggattac
     3241 ctacggcggt aagttcaaag taaaattcag attatataag aaagacaaga ttcataacag
     3301 gatagaaagc gttagagaag cccaggatgt atatctcggg cttatgccct tgatgacaga
     3361 acgcggtaca tttatcatta atggaattga aaggactgtt gttaatcaaa tacagaggtc
     3421 tccgggggtt tattttaagg aagaagaaga aattggacag cgacccacat tttctgcgcg
     3481 tatttttccg gctcgtggtc tatggatgga atttcatcca gagttacatc ataatataac
     3541 atgtctttat gcatattttg gtaagaggaa ggtctatgca acaacgcttc ttaaggctct
     3601 cggatattcc ccttttgaga ttccatctct gttctatgag gcacctgaca gggttcccaa
     3661 tgacagtatt ataaagacaa ctcttgaaaa ggattctgac attaaaaccc ccgaagacgc
     3721 catcaggaaa atctatcttg agttaagacc tggttttccg atggaagaaa gggaagcaaa
     3781 aagttttttc aaaagattat tctttacatc tgaacagtac gacctttcac ccgcaggaag
     3841 gatgcagata aacaagaaac ttggtttgaa tataaaagag cagcatctta cagtagatga
     3901 tattataaaa accataaaat atttactcaa tcttatggaa aaaaatcagg gtgaaaccga
     3961 tgatatagac caccttggta ataaaagggt aagaacatcg gctgaacttg tgaaagagca
     4021 tgtatatgaa ggattggtga ggcttgctca ttttgtaaag gaaagaatgg caattattga
     4081 taaagcacag gatatttcac ctcaggatat tttgaatagc aggatttttt ctacagtagt
     4141 tgaagaattt tttgcccgca atcagctttc tcagtttctt gaccaggtga atccgctttc
     4201 tgagcttaca aacaaaagaa gagtaactgc tgttggtgag ggcggtgtga agaaaagaaa
     4261 acgggcaggt tttgaggtaa gggatgttca ctatacacac tttggaaggg tctgtccgat
     4321 agaaacgcca gaaggcgcaa atgtcggttt gattaattcc ttgacagtat atgctgaagt
     4381 tgatggtatg ggttttatca gaacacctta ctacagggtc aaaaacggta gaattctgtt
     4441 taatgaaatc gttcgcgtat ccgcggacga agaagataat tatgttgtcg caccaccgga
     4501 tgttcctttt gatccgaaga caggagcaat tattcctgaa acaatacgag tgaggtccaa
     4561 gggtggagag tttactgaag ccagcaaaga tcaaattgaa cttataggtg tttctccaaa
     4621 acaggttgtt agtttgagtg cctctctagt tccattcctt gaacacaacg actcaaaccg
     4681 cgcattgatg ggttcaaaca tgcagagaca ggcagtacca ctgttgagaa ctgagtctcc
     4741 gcttgtacgg acaggcatgg aaaaaaaagt tgtcatggat tccggtgttg tggttaaggc
     4801 gatcaattca ggagttgttt cctatgttga cgcaagaaaa ataattattg aacgtgatga
     4861 aaaaggcata tttccatggc agcatcacga tgtttattac cttcagacat ttcgcaggac
     4921 gaaccaggat acatgttttc atcaaaaacc tgttgtttct cctggtcaga aagtgaagaa
     4981 gggccagatg atttctgatg ggcctgctgt gtcattggaa ggagacctgg ctcttggacg
     5041 caatctactt gttgccttca tgccctggcg tggttataac tttgaagatg ctatcgtgtt
     5101 gagtgaaaga cttgtgaaag aagacacgtt tacttcaatt cacatcgaaa gatttgaggt
     5161 gaccgcacag gaaacagaac gcgggcctga agaaataaca gcggatcttc cgaatgtttc
     5221 agaagaagaa cttagaaacc ttgataaaaa tggtattgtc aggataggcg cagaggtaaa
     5281 gcagggagac atacttgttg gtaagatcac acctaaaggt gaagcagaac tgacgccaga
     5341 agagaaactg ttaagggtga tatttggtga aaaggcgaga gatgtagcaa acacttctct
     5401 tactgtgcca ccagggattc atggtgtagt tgtaaatgtc aaaacttact atccttctga
     5461 caggatacca gaagaagata taaaaaaacg tattgatgaa gtggaaaagg aactgaaaag
     5521 aaagaaaaat attgttcgta aaattattac cgatcagata aaaaatattc tttccagtca
     5581 caggatttct ttggcaagat tgggcacaag ttctgaaagc tgggataaaa ttgttaattc
     5641 aatagatagg gataacgtaa aggatgaagt cagaagaatt gttgagatag gtaaaaagga
     5701 aattgaaaaa cttgagcacg atgcaaaaaa cgaagaaata aaaatccgta gaggttctga
     5761 acttgagcca aatgtgatat gcaaggtttc tgttgaaata gcaataaaaa agaaagtggc
     5821 tgttggtgac aagatgagtg gtagacatgg gaataagggt gttatttctg tagtgcttcc
     5881 agaagaggat atgccatttc ttgaagacgg tactccggtt gacattgttt taaatcctct
     5941 tggtgttcct tcccgtatga atatcgggca aattctcgag acacaccttg ggtgggcatt
     6001 gaaaacactg ggctattcaa tggaaacgcc tgtttttgaa agtttgaaag agatggaaat
     6061 aacacagaag ctcagggaag caggattgcc tgaagatggc aaaaccatgt tatatgacgg
     6121 gcaaaatggt gaaccatttc ttcagccagt gactgttggt tacatatata tgatgaaact
     6181 tattcacctg gctgacgaga aatttcatgc tcgctcggtg ggttcatatt cattgataac
     6241 ccagcaaccg cttggcggaa gagctcagtt tggtggtcag agatttggtg aaatggaagt
     6301 ctgggcgctg gaaggatatg gtgctggcca tatgcttcgc gaaatactga caataaaaag
     6361 cgatgatata aaaggacgca agaaagcgta tgagagtatt gtgaaaggat tggattatca
     6421 ggaggaaaat cttcctgaat ctttccatgt attgaccaag gaattgaatg ggctgggttt
     6481 tgatttgaga attgaaagga aaagaaaatc tgatgaagaa aaagatgtcg tgacgataag
     6541 aatcgcaccg ccaatggtca ttcggtcgtg gtcctatgga gagattaaga aagcggagac
     6601 attgaactat agaaccaata agccagaaag agatggtctt ttctgtgaga aaatctttgg
     6661 tcccgtaaaa gattgggaat gtgcctgtgg caaattcaag aaacagaggt atcgcggtgt
     6721 tatttgcgac cgatgtggtg ttgaagtaac gacatctgat gtgagacgtg aaagaatggg
     6781 gcacattgaa cttgccacac ctgtttcata tgtatggttg tttaaaagtt ctgcaaactg
     6841 gttaggagtg cttcttgatc tgacccagat gacgcttgaa aaggttcttt actacgatcg
     6901 ttatattgta gtggatcccg gtgatactgg tttgaaagaa aaggaacttc ttacagagga
     6961 tgaatacaga gaaaatcttg gtaaatacgg caataaattt cgtgctgaaa ttggtgcttc
     7021 agcaatctat gaacttctga aacgaattga tctcgaaaaa gagatgtcga aggtgaaaga
     7081 acaattgaag gctaagaaaa ctaaaaagga tcacacaggt agacgtaatc ttgtgaagaa
     7141 actgcgtatg atagagggtt taataaaaac agaggtgaaa ccagaatatc ttgtaacttc
     7201 tattattcct gtaataccac ctgatttaag accacttctt ccacttgatg gtggtaagtt
     7261 tgttgcttct gaccttaacg acctttatca gagagtgatt aacagaaata accgtcttaa
     7321 gaaattaatt aaacttcagg cccctgacat tatcatcaga aacgagaaaa gaatgcttca
     7381 ggaggcagtg gatgcccttc tcgacaatgg tagatacggc acacctgtgc tgggtaaggg
     7441 caagagacct cttaagtcac tcagtgaatc cttgaggggg aaacagggaa gattcagaca
     7501 gaatcttctt ggtaaaagag tagattattc tggaagggca gttattgttg ttgacccaaa
     7561 actgaaatta catgagtgtg gtattcccaa gcaaatggct ctgga
//
LOCUS       DAORYU010000049         7519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_6959_length_7519_cov_4.168944, whole genome shotgun sequence.
ACCESSION   DAORYU010000049 DAORYU010000000
VERSION     DAORYU010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7519
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6959_length_7519_cov_4.168944"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="PLW07_02630"
     CDS             <1..589
                     /locus_tag="PLW07_02630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOL49449.1"
                     /translation="EILDSVNKAVTVWGSARVSSDDQWYREAENLGKILVKNGYAVIT
                     GGGPGIMEAANKGAFEAGGKSIGLNIELPHEQKPNQYISHLITFRYFFVRKVMFVKYA
                     TAFVVFPGGFGTIDEFTEAITLVQTGRVKKFPVILVNKNHWDPLIAWMKDYLIKKSYI
                     DKNDLGIFSVVDTSEQVLKIIKNFYHKKRHGDQSD"
     gene            complement(543..1895)
                     /gene="glmM"
                     /locus_tag="PLW07_02635"
     CDS             complement(543..1895)
                     /gene="glmM"
                     /locus_tag="PLW07_02635"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR03990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOL49450.1"
                     /translation="MDNLIISISGIRGIYGKSLTEDIAVRAGRAFGLWTNTENIVVGR
                     DTRVSGNSLMQSFMAGVSSAGKRVFDCGIVPTPALTWFIEKHHNFSGTVITASHNPVE
                     YNGIKLISPSGTFLNPVEFKDFLDIYNKTDILDIKGRAEIMGEKKDLMDNFFSCLFNY
                     IDVSAIRKKRFKVVVDPVQGVGALYSKKFLEMLGCEIKIINENPLGKFSHQPEPVPSH
                     LRKLSYVMRIENADIGFAQDPDCDRLVLVEKSGKFVSEEQGLAISVYWMLHRKKGPVV
                     VNIATTRLVDDICKEFKTQLFRTKVGEVFVVEEMKRVGAVAGGEGNGGIILPEFHYGR
                     DSYIGMGLILEMMAKTSLSLSEIIEKFPRYKFVKRKISFPRERIECLYKSLEKEFSSG
                     KINYLDGFRIDFDDTWLGIRSSGTEPIIRIFVEGKKQSRIYRIINLIDRHVASCGRNF
                     "
     gene            complement(1897..2595)
                     /gene="truB"
                     /locus_tag="PLW07_02640"
     CDS             complement(1897..2595)
                     /gene="truB"
                     /locus_tag="PLW07_02640"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOL49451.1"
                     /translation="MDKQLDGFLNINKPSGITSYDVIRFIKKIPGFNARIGHTGTLDP
                     LASGVLVVCIGTATRKAGKFLAMEKEYLATLLLGITTDTDDIQGEILESSQVNPESID
                     EQQVYKTICSFAGEIEQVPPSVSALRRHGVRLYKLHRQGKPVIPEPRKVFVHEITVEK
                     IEIPRIVFRVVCSRGTYIRSLCRDIGKKLGYGAAQESLIRLRIGEFTIDNAVELKQLN
                     EENLKKYIIPSSVI"
     gene            complement(2598..3596)
                     /locus_tag="PLW07_02645"
     CDS             complement(2598..3596)
                     /locus_tag="PLW07_02645"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOL49452.1"
                     /translation="MLVSEHDRRIVRKICDEIVKHDSFFLTTHQVPDGDGLSCELAFS
                     LILRKLGKRASIVNEMLPSSIYSFLPGYSDILRIEKYAEMKSVPGVAIVFDCSNKDRI
                     GRPLSFIPESVVMINIDHHQGNTQFGDINWVCENCSSVGEMCFSILEELNCIDKEVAE
                     CLYVAILTDTGSFRHHFDTRTIRITGKLLSTGINPERIADYIYHSYPVSTLRLLGHAL
                     VSFQYDMRLRTAWAIIDQQILVKTGAFEQDAEIIIDALRSIGNVDFVFLVKERKNEIK
                     FSLRSKKGFNVRLIAEKFGGGGHDGAAGFSMTGIPLTDAVEKFLRYLRKHHSKRAG"
     gene            complement(3574..4707)
                     /gene="hflX"
                     /locus_tag="PLW07_02650"
     CDS             complement(3574..4707)
                     /gene="hflX"
                     /locus_tag="PLW07_02650"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOL49453.1"
                     /translation="MQHPNILTGTDIAGKRAIVIAFKEKFFDQEKENLKELESLACTL
                     GLNVVKSLLIRHPDPIYPSTYIGKGKIQEICKIVQEMNCDVVIFEKELTPVQQRNLEE
                     SFSIAVIDRTVLILLIFGKHAHTLEGKLQVELAQMTYLLPRLAGSSGRLSRLGGTIGT
                     RGPGEQKLEVYRRRARERIRLLNVRIKEIEKHRNIIRETRQKKNLPVAAIVGYTNAGK
                     STLLNALTHKQDAIVDNKLFATLDPLTRLVFLGDGTFCLISDTVGLLSSLPHHLIAAF
                     RATLEELKYAYLLICLVDAASFSIEKQISTIDNVFEIMSIQQKPRINVFNKIDLIDET
                     ELGFLKRNYPDSLFISAKNSTGFHELKSAIKEIVREHACFRTR"
     gene            complement(4694..5020)
                     /gene="rplU"
                     /locus_tag="PLW07_02655"
     CDS             complement(4694..5020)
                     /gene="rplU"
                     /locus_tag="PLW07_02655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOL49454.1"
                     /translation="MKAIIGLSGKQYCVKEGDTFEVFKLSHQKGNQFTIQDILAIEKQ
                     GSLITDKEFLKNCAVIAEVIDEKKGEKLYVFKKKKKTGYSKKTGHRDILSVVKIVRIQ
                     TSDAAS"
     gene            complement(5017..>7519)
                     /locus_tag="PLW07_02660"
     CDS             complement(5017..>7519)
                     /locus_tag="PLW07_02660"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOL49455.1"
                     /translation="LNNTIQDILIRWKRMAGFNALWVPGTDHAGIATQNVVEKELMKK
                     GLTREALGREKFLEEVWKWRDAYGNRIIEQLKRLGASCDWHRTCFTMDKNFSQAVQTV
                     FIKLYEKGLIYRGTRIINWCPRCTTALSDEEVEYREENGFLYYLKYPVEENGYLVVAT
                     TRPETMLGDTAVAVNPSDERYKDFIGTKVHLPFVNRLIPVIADTQVDMEFGTGAVKVT
                     PSHDPADYSIAKRHNLEFLTVMDETAKMNENAGIFKGLDRFECRRKIIEHLQEQKLIE
                     KIEPYSHRIGHCYRCDTLIEPYISRQWFVDMKKLAEPAIKVVLDGGLKFYPERWTKIY
                     LQWLYNIQDWCISRQIWWGHRIPVWYCSNSDCPPVVSISTPRECPCCNGRNFVQDDDV
                     LDTWFSSWLWPFAVFGWPEKTKDLEYFYPTNTLVTAQEILFFWVARMVMAGFEFMEEK
                     PFESVYIHGTVRDETGRKMSKSLGNAVDPIEIIDEVGADALRFSLISLTSFGQDVFLP
                     EKFHYKGRNFINKIWNSFRYLEMLVEKNNCEMISTEISGQKFLKDLGLAEKWILTLLN
                     TTTGHVNKCLEEFKFNEAADSLYEFFWHQYCDWYVELSKMYSEKDTYLVCRIIPVMVF
                     VMMRTLKLLHPFVPFITEELWNRLKKITYIDDAFITKSTWPIPDASFEFPEAVAEMEE
                     IKKLVIEIRDIKTTFKIPVTKNMEAIYRGISLLNFDEARQKVEFLSKTVFLYDNEAVG
                     YSEDMLVRVWNDGWFAIKVKGLLDINAEKQKLLKEKDKVEKILTSTEKKLSNPDFLKH
                     APADVVEKTKKLCDDLAAELTKIEKNFQLLTREKK"
BASE COUNT         2266 a         1618 c         1175 g         2460 t
ORIGIN      
        1 tgaaatcctt gacagtgtga acaaagcagt tactgtctgg ggctctgcca gggtttcctc
       61 agatgaccaa tggtacagag aagcagaaaa cctcggaaag attcttgtaa aaaatggata
      121 tgcagtcatt acaggtggtg gaccaggcat catggaagct gcaaataaag gcgcctttga
      181 agcaggtggc aaatccattg gtctgaacat agaattacca catgaacaaa aaccaaatca
      241 atatatttcc catctcatta cgtttcggta tttttttgta aggaaggtaa tgtttgttaa
      301 atatgcaacc gcatttgttg tattccccgg tggctttgga acaattgatg aatttacaga
      361 agccattaca ctggtgcaga caggaagagt caaaaaattc cctgtaatac ttgttaataa
      421 aaaccactgg gacccattaa ttgcctggat gaaagactat cttataaaaa aatcttacat
      481 tgataaaaac gatttgggaa tttttagcgt cgtggatact tctgaacagg ttttgaaaat
      541 tatcaaaaat ttctaccaca agaagcgaca tggcgatcaa tcagattaat aattcggtaa
      601 atcctgctct gttttttccc ttcaacaaag attcttataa ttggttcagt acctgaagat
      661 cttatgccta accatgtatc gtcaaaatca attctgaaac cgtctaaata attgatctta
      721 ccacttgaaa attctttctc aagggatttg tacaaacatt cgattcgctc tctggggaaa
      781 gaaatttttc tctttacgaa tttatatctg ggaaattttt caataatttc tgataaagat
      841 agtgaagttt ttgccatcat ttccagaatc aaacccattc caatataact atcacgacca
      901 taatgaaatt caggtaatat tatcccacca ttgccttccc caccagccac agcaccaacc
      961 cttttcatct cctcaacaac aaaaacttct cccacctttg ttctaaacag ttgtgttttg
     1021 aattctttac atatatcgtc aacaagcctt gttgtggcaa tattcacaac aacaggacct
     1081 ttttttctat gaagcatcca gtaaactgag attgcaagac cctgttcttc gctaacaaac
     1141 tttccacttt tttcaaccag taccagacga tcgcaatccg gatcctgagc aaatccaata
     1201 tcagcatttt caatcctcat cacatacgaa agttttctga gatgggatgg aactggttct
     1261 ggttgatgag aaaatttgcc cagagggttt tcattaataa tttttatttc acaacccagc
     1321 atttccagaa atttttttga atacagggcg cccacaccct gaaccgggtc aacaacaacc
     1381 ttgaatcttt tttttcttat tgctgataca tctatgtagt tgaacagaca ggagaaaaaa
     1441 ttatccatca aatctttttt ttcacccata atttcagccc tgccctttat atctaaaata
     1501 tcggttttat tgtatatatc aagaaaatct ttgaattcaa caggattaag aaaagtaccc
     1561 gatggactaa ttaattttat gccattatat tcaacagggt tatgactcgc tgtaatcacg
     1621 gtaccggaaa aattatgatg tttttcaata aaccaggtta atgctggggt tggcacaatc
     1681 ccgcagtcaa aaacacgttt tccagcagat gaaacccctg ccataaatga ctgcataagg
     1741 gaattcccgc taacccttgt atctcttccg acaacaatgt tttctgtatt tgtccacaac
     1801 ccaaatgccc tgccggcacg aaccgcgata tcttctgtca gagatttccc gtaaattccc
     1861 ctgatacccg aaatactgat gatcaaatta tccatcttaa atcactgaag aaggtataat
     1921 atattttttt aagttttctt catttaattg tttcaattca acagcattgt caattgtaaa
     1981 ttccccaatc cgcaatctga ttaaagattc ctgggcggct ccatatccaa gttttttgcc
     2041 aatatctctg catagagacc tgatatatgt acctcttgaa cacaccaccc tgaaaacaat
     2101 cctcggtatt tctatttttt ccaccgttat ttcatgaaca aacacctttc gtggctcagg
     2161 aatcactggt ttgccctgcc tgtgaagttt ataaagtcgt acaccatgcc tgcgtaatgc
     2221 tgaaacactg ggaggaacct gttcaatctc gccggcaaaa gaacaaattg ttttataaac
     2281 ctgttgttca tctattgact ccggattaac ctgtgatgat tcaagaattt ctccctgtat
     2341 gtcgtcagta tcagttgtga taccaagaag caatgttgct aaatattctt tctccatggc
     2401 taaaaattta cctgccttcc ttgtcgcagt gccaatacag acaacaagaa caccagaagc
     2461 aagaggatca agtgttcctg tatgcccaat tctggcatta aatcccggaa ttttcttaat
     2521 aaatcttata acatcatatg acgttattcc tgatggttta ttaatattca aaaatccatc
     2581 gagttgttta tccattttca acctgccctt ttagaatgat gttttcttaa gtatcgtaaa
     2641 aacttctcaa cagcatctgt tagcgggata ccagtcattg aaaatccagc tgcaccatca
     2701 tgtcctccac caccaaattt ctctgcaatc aaacgaacat taaaaccttt tttgctcctc
     2761 agactgaatt taatttcatt ttttctttct ttcacaagaa aaacaaaatc aacattaccg
     2821 atagaacgta aagcatctat aataatttct gcatcctgct caaaggcacc tgtttttacc
     2881 agtatctgct ggtcaataat tgcccaggcg gtacgcaaac gcatatcgta ttgaaatgat
     2941 acaagggcat gccccagaag cctcaacgtt gaaaccgggt aactgtgata aatgtaatct
     3001 gcgatacgtt cgggatttat tccggtactc aacaattttc cagtaatcct gattgttctg
     3061 gtatcaaaat ggtgtctgaa tgaacccgta tcagtcagga ttgcaacata taaacactct
     3121 gcgacctctt tatcaatgca gttgagttct tccagtatag agaaacacat ttcaccaaca
     3181 gatgaacaat tttcacaaac ccagttaata tctccaaatt gagtatttcc ctgatgatgg
     3241 tctatattaa tcatcacaac agattccggt ataaaagata aaggtctacc tattctatct
     3301 ttgttgctgc aatcaaaaac aatggcaaca cccgggactg atttcatttc cgcatatttt
     3361 tcaatcctca aaatgtctga gtatcctgga aggaaactat aaattgatga gggcaacatt
     3421 tcattcacaa tactggctct ctttccaagt ttacgcaaaa tgagagaaaa agcaagttca
     3481 caacttaatc catcaccatc aggaacctgg tgggtggtaa gaaagaaact atcatgtttg
     3541 actatttcat cacagatttt acgaacgatt cttctatcgt gttctgaaac aagcatgttc
     3601 cctcactatt tccttaatcg cactttttaa ttcatgaaat ccagtactgt tttttgcaga
     3661 aatgaaaagc gaatcggggt aatttctctt aagaaaccct aattctgttt cgtctattaa
     3721 atctattttg ttgaacacat taattcttgg tttctgttgt atcgacataa tttcaaaaac
     3781 attatcaatt gtactgattt gtttttctat actgaaactt gctgcgtcaa caagacagat
     3841 caaaagataa gcatatttta attcctcaag ggtcgccctg aaagcagcaa tcagatggtg
     3901 tggtaaagaa gaaagcaatc ccacggtatc agatatcaaa caaaaagtcc catctccaag
     3961 aaaaacaagt cttgtaagag gatcaagtgt tgcaaacaac ttattgtcaa ctattgcgtc
     4021 ttgcttatga gttaacgcat taagaagtgt acttttgccg gcgtttgtgt agcccacaat
     4081 agcagccaca ggcaagtttt ttttctgtct tgtttcacgg atgatgtttc tgtgtttttc
     4141 tatttctttt attctcacat ttaacaatct tattcgttct cttgctcttc tcctgtaaac
     4201 ttcgagtttt tgttctcccg gacctcttgt accaattgtt ccaccaagtc gtgaaagcct
     4261 tcctgaagaa ccagcaagtc gtggtaaaag atacgtcatc tgggcaagtt caacctgtaa
     4321 ttttccttca agcgtatgag catgtttccc aaaaattaag agtattagta ctgttctatc
     4381 aataacagca atgctgaatg attcttcaag atttctctgt tgaacgggcg taagttcttt
     4441 ttcgaaaatt acaacgtcgc aattcatttc ctgaacaatt ttgcaaatct cctgaatttt
     4501 tcccttccca atataagtcg aaggatagat agggtctgga tgtcttatca ataaagattt
     4561 tacaacattc aatccaaggg tgcaggcaag cgattcaagc tcctttaaat tttccttttc
     4621 ctgatcaaaa aacttttcct taaaggcaat gacaatcgct cttttaccag caatatccgt
     4681 acctgtcaga atattaggat gctgcatcgg aggtctgtat cctgacgatt ttcacaacag
     4741 aaagaatatc tctatgtcct gttttctttg aataacctgt ctttttcttt tttttgaata
     4801 catatagttt ctcacccttt ttttcatcta tcacttcagc aataacagca caattcttaa
     4861 gaaactcttt gtctgtaatc aaagaacctt gcttttcaat cgccagaatg tcctgtatcg
     4921 taaactgatt tcctttttgg tgtgaaagtt taaatacttc aaacgtatct ccttccttca
     4981 cacagtattg ttttccactc aatccaataa ttgctttcat tttttctccc tggttaaaag
     5041 ttgaaagttt ttctcgattt ttgtcagttc agcggcaagg tcatcacaaa gtttttttgt
     5101 tttttcaaca acatctgctg gcgcatgttt aagaaaatca ggatttgaaa gtttcttttc
     5161 tgtggaggtt agaatttttt ccactttatc tttttctttt aacaactttt gcttttctgc
     5221 atttatatcc agtagacctt tgacttttat cgcaaaccac ccatcattcc agacgcgaac
     5281 aagcatatct tctgaataac caactgcttc gttgtcatac aaaaagactg ttttagaaag
     5341 aaactcaact ttctgtcttg cttcatcaaa attaagtaac gaaatcccgc gataaatagc
     5401 ttccatattt tttgtaaccg gtattttaaa agttgtcttt atatctctga tttctatgac
     5461 aagtttctta atttcttcca tttctgctac ggcttcagga aattcaaaac tggcatcagg
     5521 aatcggccag gtacttttcg tgataaatgc atcatctatg tacgtaattt ttttcaatct
     5581 attccagagt tcttcggtaa taaatgggac aaaaggatga agtaatttta atgtcctcat
     5641 cattacaaaa accattacag ggattattct gcaaacaaga tatgtatcct tttcagaata
     5701 cattttggaa agttctacat accaatcaca atactgatgc cagaaaaact catatagaga
     5761 atcggcagct tcgttgaatt taaactcttc caaacacttg ttaacatgcc ctgttgttgt
     5821 gttgagtaaa gttagaatcc atttttctgc aagcccaaga tctttcaaga atttttgtcc
     5881 tgaaatttct gtagaaatca tttcgcagtt gttcttttcc accagcattt caagatatct
     5941 gaatgaattc catattttgt taataaaatt tcttccctta taatgaaatt tctcaggaag
     6001 gaaaacatcc tgcccgaacg aagttaatga aatcaaactg aaacgtaatg cgtcagcccc
     6061 aacttcatca ataatttcaa tgggatctac tgcattgcca agagatttac tcatctttct
     6121 tcctgtttca tctctcactg taccgtggat gtatactgat tcaaaaggtt tttcttccat
     6181 aaattcaaaa ccagccatta ccatccttgc aacccagaaa aacagaatct cctgggcagt
     6241 aacaagggta tttgtgggat aaaaatattc aaggtccttt gttttttctg gccatccaaa
     6301 aacagcaaat ggccacagcc aggaagaaaa ccatgtatcc agaacatcat catcctgtac
     6361 aaaattccta ccattacaac acggacattc tctgggtgtt gatatgctta caacaggcgg
     6421 acaatcactg tttgaacaat accatacagg tattctatgt ccccaccata tctgacggct
     6481 aatgcaccaa tcctggatat tatagagcca ttgaagatat atctttgtcc atcgttctgg
     6541 ataaaattta agtccaccat ccaataccac ttttatagcg ggttctgcaa gtttcttcat
     6601 gtcaacaaac cattgtctgg aaatataggg ctctataagt gtatcgcaac gataacaatg
     6661 acctatgcga tgactatatg gttcgatttt ctcaataagt ttttgctcct gaagatgttc
     6721 gataattttt cgacgacatt caaacctatc cagtccttta aaaattcctg cattttcatt
     6781 catttttgcc gtttcatcca tcacagtcaa aaattccaaa ttgtgtcttt ttgcaatcga
     6841 ataatctgca ggatcgtgag aaggtgtcac ctttactgct cctgttccaa attccatatc
     6901 aacctgcgta tctgcaatta caggaatcaa tcggttcaca aaaggaagat gtacctttgt
     6961 accaataaaa tctttgtatc tttcatctga aggattgact gcgactgcag tatcacccag
     7021 cattgtttca ggtctcgttg tagccaccac aagatagccg ttttcctcaa ctggatattt
     7081 caggtaataa agaaatccat tttcttccct gtattcaact tcttcgtctg ataaagctgt
     7141 tgtgcatcgc ggacaccagt ttataattct tgttcccctg taaataagtc ccttttcata
     7201 caattttatg aacaccgttt gaacagcctg agaaaaattt ttatccatcg taaaacaggt
     7261 tctatgccaa tcacaggaag caccaagccg cttcaattgt tcaataattc tattcccata
     7321 tgcatcgcgc catttccaca cttcttcaag aaatttttct ctaccaaggg cttctcttgt
     7381 caatcctttt ttcatcagtt ctttttcaac aacattctgt gttgcaatac ctgcgtgatc
     7441 tgtacctggc acccataaag cattgaaacc cgccattctt ttccatcgta tcaaaatatc
     7501 ctgaattgtg ttgtttagc
//
LOCUS       DAORYU010000050         7478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_7012_length_7478_cov_4.167453, whole genome shotgun sequence.
ACCESSION   DAORYU010000050 DAORYU010000000
VERSION     DAORYU010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7478
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7012_length_7478_cov_4.167453"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /gene="murI"
                     /locus_tag="PLW07_02665"
     CDS             <1..799
                     /gene="murI"
                     /locus_tag="PLW07_02665"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOL49456.1"
                     /translation="AIFDSGIGGLTVAKKIHCLLPGEKIIYFGDTARVPYGTKSPETI
                     RQYAIQIAGFLTRFDPKLIVIACHSVSSLGRKFLENRFSKYHFVDVIHPSIEEAINIT
                     KNKKIGVIGTPATIASGRYPEIIRKIDRKMCVYQASCPLLVPLVEEGWFKGDVTEAVL
                     EYYISDLLKKKIDTLILGCTHYPMLKKTIQKIVGNSVNLVDASDATARKVKFFIIKNA
                     LSNSYKRKFPTLYFSDLPEYRKKIMNRFWKTDNFMIKKINIERSFNV"
     gene            792..1157
                     /gene="queD"
                     /locus_tag="PLW07_02670"
     CDS             792..1157
                     /gene="queD"
                     /locus_tag="PLW07_02670"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HOL49457.1"
                     /translation="MFELTVSSYFSGAHRLRNYKGKCENLHGHNWKVDVTVGGEIDGS
                     GMVIDFGILKEILEQVLSKLDHKYLNEIDYFKKVNPSSENAALYIFKQLEKKLKGYNV
                     KVKKVVVWENEKQCASYFE"
     gene            1210..1713
                     /locus_tag="PLW07_02675"
     CDS             1210..1713
                     /locus_tag="PLW07_02675"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOL49458.1"
                     /translation="MTNQTLRDFAVDFETKGVNLYMSLASKTSNLLGKELFYSLAEQE
                     VQHARLIERVYIASNVKQWVSNTSDIEAELKSFWDNAQQLNLKQDPGDLSGYELAMEM
                     ELKSISSYQQFLKKTDNEQEKKFLTWVIEEEKKHLDALRNVYYFLTSTGDWFQNEESK
                     IWNWMNQ"
     gene            1726..2604
                     /locus_tag="PLW07_02680"
     CDS             1726..2604
                     /locus_tag="PLW07_02680"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019999734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOL49459.1"
                     /translation="MKAISDVVLPVPKIYSGKVRELFEIDSKRILIVTTDRISAFDFV
                     LPNPIPEKGIILNQLSLFWFEKLKNVCENHLDIIDFEQFPQNLNGFRNVLEKRSVIAK
                     KTKKIPIECVVRGYISGSGWKEYSESGTICGIKLPAGLRESEKLPEPMFTPATKEEKG
                     AHDRNINFDEMIEIVGKEVASHLKDISIALYKQASEYAERKGIIIADTKFEFGLYDGK
                     IVLIDEILTPDSSRFWEKAAYEAGRSQDSLDKQFVRNYLESICWNKQPPVPELPVDVI
                     EKTREKYWQIYRILTN"
     gene            2616..3101
                     /locus_tag="PLW07_02685"
     CDS             2616..3101
                     /locus_tag="PLW07_02685"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF014726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HOL49460.1"
                     /translation="MKIKSENVYVVEIGLRKGLFDAFGNEVAHSIRELGINGIDRVDV
                     YDVYRVYGDVNFSLVRRIAKDLLLDPVAQTMKIYRCGEKQKSSCISVEVWYKQGVTDP
                     VAMTAIKGIKDLGIKNEVKINCGKKYEFHGSKLNRGLIEMICTKILANTLIQDYIIRG
                     I"
     gene            3103..5478
                     /gene="purL"
                     /locus_tag="PLW07_02690"
     CDS             3103..5478
                     /gene="purL"
                     /locus_tag="PLW07_02690"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HOL49461.1"
                     /translation="MAQATIIEICHATDTRLIEISKKGLLSLTLDEMKRIQRYFLSAG
                     RNPTDLELEMIAQTWSEHCFHKTFKSRIQLTEILDSDSPVTEEIPSLFALIRQATDNI
                     KKNWCISVFKDNAGIIRFDRDNCVCFKVETHNHPSALEPYGGAGTGIGGVIRDILGAG
                     LGAKPILNTDIFCFAPLDTRHEELEEDIFHPRRLFKGVVSGVRDYGNRMGIPTANGAI
                     LFDPGYLHNILVYCGTVGMMPRSAVNKKVRSGDLVVVIGGRTGRDGIHGATFSSASLE
                     KDIPTSVVQIGNPIVEKKMLDALMMARDENLYSAITDCGAGGLSSAVGELAQHCGAKV
                     HLDKVPLKYKDLKPWEIWVSESQERMVLSVPPSKFNRLKEILESEDVEYACIGTFNST
                     GKITIYHEKDLVGELDLDWLFQKKDLPVLKALYEKRETTNIIPENVNFEDTLLQLLAD
                     PNIASKEIVVRQYDHEVQGHTIIKPFGGVDGCSASDGVVLAPVYGSNRGIVVSCGINP
                     WYGMLDPYYMAGNCIDEALRNIVACGGNPDRTAILDNFCWGKIDDPVELGKLTRCVKG
                     CRDYAIAYNVPFISGKDSLNNYFTKSDGSIVSIPGTLLISAISIIDDVQKTTGSDFKE
                     AGNLIYVLGETFDELGGSRFLSIFNLEGGRCPVPNPEISLAIMRNLHQAIKNNLIMSC
                     HDCSEGGFAIAIAEMMIGSGYGAEVYLDRMPATTEETVSLLFGESQGRFVVEVDEACV
                     EKFEKLFKDLPCKQVGRVIPDFRLKVNHRDNMLLDVDGEMMRQRWSGAISW"
     gene            5472..6236
                     /gene="purQ"
                     /locus_tag="PLW07_02695"
     CDS             5472..6236
                     /gene="purQ"
                     /locus_tag="PLW07_02695"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF024897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HOL49462.1"
                     /translation="MVKVIILRTGGTNCDLETEWACKTAGASVSRVHISQITSGRVKI
                     LDFDVLIVPGGFSYGDDLGAGKVLANEIMLKCQEQIEKFREKQRPIIGICNGFQVLVK
                     AKMLPCWGKHSASLVWNDSGRFEDRWVHLKVEKSISPFFENLPEIIRFPVAHAEGKFV
                     VKTDSILKKIEKNQQVVLRYVDPSGEIKGYPYNPNGSIAGIAGVCDEKGLVVGMMPHP
                     ERALLTLHLPDWLRLRQRQEFSYGYQFFKNVIEYCL"
     gene            6263..7153
                     /gene="argB"
                     /locus_tag="PLW07_02700"
     CDS             6263..7153
                     /gene="argB"
                     /locus_tag="PLW07_02700"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOL49463.1"
                     /translation="MVENSIHAKAETLIEALPYIQDYKGKTFVIKIGGAILRLPESKK
                     NILQDIAFLSAVGIKTVVVCGAGPTITEEIEKRGKKAKFIEGLRVTDRETLDIVVEFL
                     GAARDEVVETLKNEFRTEAFSLKPEDRHLIAKKIHWQKGDEIIDLGFVGQVENVQADV
                     LASFLEKGVAVFAPIGISLDGQKYNINGDSVSSAVAQSLGAEKLIFVTGVRGVMRNPD
                     NPDTLLSVITASQGENLIKDEVISGGMIPKIRGAIGSLKGGVKKVHIISGNVAHSIIL
                     EVFTEQGIGTEIILDGENNG"
     gene            7146..>7478
                     /locus_tag="PLW07_02705"
     CDS             7146..>7478
                     /locus_tag="PLW07_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOL49464.1"
                     /translation="MDEITVKQYYNDYIIGCYGKENFVFVKGKGPWLWDINGKKYLDF
                     FPGWAVSGLGHCPTRVVKALTKQLKTLMHVANNYYIDVQALCAKKLIEISFDGKVFFC
                     NSGAEANEA"
BASE COUNT         2360 a         1184 c         1759 g         2175 t
ORIGIN      
        1 agcgattttt gattctggca taggcggatt gactgttgca aaaaaaatac attgtttgtt
       61 gccaggagaa aaaattattt attttggcga taccgccaga gttccatatg gcacaaaatc
      121 acctgaaaca ataaggcagt atgcaatcca gattgccgga tttcttacca gattcgaccc
      181 caaacttatt gttattgcct gtcattctgt ttcttcgctt ggcaggaaat ttcttgaaaa
      241 ccgttttagc aaatatcatt ttgtagatgt gatacatcct tcaattgaag aagcaattaa
      301 cataacgaag aataaaaaaa ttggtgttat tggtactccg gcgacaattg caagcggaag
      361 atatcctgag attattagaa agattgacag gaaaatgtgt gtgtatcagg catcctgtcc
      421 tttactggta ccgcttgttg aagagggctg gttcaaagga gatgtaacag aagcagtgct
      481 tgaatattac atttcagacc ttttgaaaaa aaagatagac accctgattc ttggttgcac
      541 tcattatcca atgctgaaga aaacgataca aaaaatagtc ggtaattctg tgaatcttgt
      601 tgatgcgtct gatgctactg caagaaaggt gaagtttttt ataattaaaa acgctctttc
      661 gaattcgtat aaaagaaaat ttccaacgct ttattttagc gatttacccg aatatcgcaa
      721 aaaaatcatg aatcgtttct ggaaaactga taattttatg atcaagaaga ttaatattga
      781 aaggagtttc aatgtttgaa ctcacagtca gcagctattt ttcaggtgcc catcggttaa
      841 ggaattataa gggtaagtgt gagaatcttc acggtcataa ctggaaggtt gatgttactg
      901 ttggcggaga gattgatggt tcaggtatgg tgattgactt cgggattttg aaagagattc
      961 ttgaacaggt gctttcgaaa ctggaccaca aatatcttaa tgaaattgat tattttaaga
     1021 aagttaatcc cagttcagag aatgcagcat tgtatatttt taagcaactt gagaaaaaat
     1081 tgaaagggta taatgtgaaa gtgaaaaaag tggttgtatg ggaaaatgaa aaacagtgtg
     1141 cctcttattt tgaataattt tctcaatgat tgttttatgg tataataaaa cacctcacag
     1201 gagcgaaaaa tgaccaatca gacattaaga gattttgccg ttgattttga aacaaaaggc
     1261 gtaaatttat acatgtctct tgcatcaaaa acatccaatc ttcttggtaa agaactcttt
     1321 tattctcttg ccgaacagga ggtccagcac gccaggttaa tagagagagt atatattgct
     1381 tcaaatgtta aacaatgggt aagtaataca tctgacattg aagcagaact aaagagtttc
     1441 tgggataacg ctcaacaact gaatttaaag caggaccctg gcgacctctc aggatatgag
     1501 ttagcaatgg aaatggaact aaaatcaatt tcttcctatc agcaatttct taagaaaacc
     1561 gataatgaac aggagaaaaa atttttgacc tgggttatag aggaagaaaa aaaacatctt
     1621 gacgcattga gaaatgttta ctactttttg accagtacag gcgactggtt ccagaatgaa
     1681 gagagcaaaa tctggaattg gatgaatcaa taaccaggag tgattatgaa agccatttct
     1741 gacgtggtac ttccagtgcc aaaaatttat tctgggaagg tcagggaact atttgagatt
     1801 gatagtaaaa gaattctgat tgtgacaaca gacaggattt ctgcttttga ttttgttctt
     1861 ccaaatccaa ttccagaaaa gggtattatt ctcaatcaac tgtcactgtt ctggtttgaa
     1921 aaattgaaaa atgtctgcga gaaccatctt gatataattg attttgagca atttccgcag
     1981 aatctgaatg gttttcgtaa tgttctggaa aaaagaagcg tgattgcaaa aaagacaaaa
     2041 aaaatcccaa ttgagtgtgt cgtgcgcggg tatataagtg gaagcggctg gaaggagtat
     2101 agtgaatcag gtactatttg cggaataaaa cttcctgcgg gtttaagaga gagtgaaaaa
     2161 ctgcctgaac cgatgtttac tcctgcaaca aaagaagaaa aaggtgcgca cgatagaaac
     2221 attaactttg atgaaatgat agaaattgtt ggcaaagaag tcgcttctca tttaaaagat
     2281 atttcaatcg ctctttacaa acaggcgtct gaatatgctg aacgtaaggg gattattatt
     2341 gcggacacaa agtttgaatt cggtctctac gatggtaaga ttgttttgat agacgaaatt
     2401 ttaacgcctg attcttcaag gttctgggaa aaagccgctt atgaggcggg tagaagtcag
     2461 gatagccttg acaagcaatt cgtacgtaat tatcttgaaa gtatttgctg gaataaacag
     2521 ccacctgtac cggaattacc ggttgatgtg atagaaaaaa caagagaaaa atactggcag
     2581 atttacagga ttctcactaa ctaactatcc gttttatgaa aattaaatct gaaaatgttt
     2641 atgttgtgga aattggattg agaaaaggat tgtttgatgc ctttggcaat gaggttgctc
     2701 attctatcag agaacttggt atcaatggta tagacagggt tgatgtgtac gatgtttaca
     2761 gggtttacgg ggatgtcaat ttttctctcg tgcgcagaat tgcgaaagac cttttgctgg
     2821 atcctgtcgc gcaaacaatg aaaatatacc ggtgtggtga aaaacaaaaa tccagttgta
     2881 tctctgttga ggtctggtac aaacagggag tgacagaccc agttgcaatg actgcgatta
     2941 aaggtataaa ggaccttggt atcaaaaatg aggtaaaaat taattgtggg aaaaaatatg
     3001 aattccatgg ttctaaactg aacagaggac tgattgaaat gatatgcacg aagattctcg
     3061 cgaatactct tattcaggac tacattatca gagggatttg aaatggcgca agcaacaatc
     3121 atagaaatat gtcatgcaac agatacccgg ttgattgaaa tcagtaaaaa aggacttctg
     3181 tcattgactc ttgatgagat gaaaaggatt caaagatatt ttctttctgc tggcagaaat
     3241 ccaactgacc ttgaacttga aatgatagcc cagacgtggt ctgagcattg ttttcacaag
     3301 acattcaaga gcagaattca gttaactgaa attctggatt cagattcgcc ggtcacagaa
     3361 gaaatcccct ctctgtttgc tcttatcagg caggcaacag ataacattaa aaaaaactgg
     3421 tgtatttctg tttttaagga caatgcaggg atcatcaggt tcgatcgaga caactgcgtc
     3481 tgttttaaag ttgaaaccca caatcatcct tcagcccttg aaccctatgg tggcgcaggc
     3541 acgggtattg gtggagtcat tcgggatatt ctgggcgcag gacttggcgc aaaaccaatt
     3601 ctaaacactg acatattttg ttttgctcca cttgataccc gtcacgaaga actggaagaa
     3661 gatatttttc atcctcgacg gttgtttaag ggtgtggtat caggcgtaag ggattatggt
     3721 aacagaatgg gtattccgac tgcaaatgga gcaattctgt ttgaccctgg atatcttcac
     3781 aacattctgg tttattgtgg tactgtggga atgatgcctc gcagtgctgt gaataaaaaa
     3841 gtcaggtcag gagacctggt ggttgtgatt ggtggaagaa ccggaagaga tggtatccat
     3901 ggagcgacat tctcatcagc aagtttggag aaagatatac caaccagcgt tgtccagata
     3961 ggcaatccca ttgttgaaaa aaagatgctt gatgcgttga tgatggcaag agatgaaaat
     4021 ctttattcag caattactga ttgtggagca ggcgggcttt catctgcagt gggcgaactt
     4081 gctcaacact gcggagcaaa ggttcacctt gataaagtac ctctgaaata taaggatttg
     4141 aaaccctggg aaatatgggt gtctgaatcc caggaaagaa tggtgctttc tgttccgccg
     4201 tcaaaattta atcgtctaaa ggagatactt gaatcagaag atgttgagta tgcctgcatt
     4261 ggcacattta attcaactgg aaagattaca atttatcatg aaaaagattt ggtgggagaa
     4321 cttgaccttg actggctttt tcaaaaaaag gatttgcctg ttttgaaagc attatacgaa
     4381 aaaagggaaa ccacaaacat tattcctgaa aatgttaatt ttgaagatac tttgctacaa
     4441 ttacttgctg acccgaatat cgcttcaaag gaaatcgttg tcagacaata cgaccacgag
     4501 gttcagggtc ataccattat caaacctttt ggcggtgtcg atggttgcag cgcatctgat
     4561 ggcgtggtgc ttgctccagt ttatggcagc aaccgtggca ttgttgtttc gtgtggtatc
     4621 aatccatggt atggcatgct tgatccttat tatatggcag gcaattgcat tgacgaggca
     4681 ttgcgcaata tcgttgcctg tggtggaaat cctgacagaa cagcaattct tgataatttc
     4741 tgctggggaa agattgatga cccggtggaa cttggtaagt taacaagatg cgtgaaaggt
     4801 tgtcgggact atgcaatagc gtacaatgtt ccttttattt ctggtaagga cagtttgaac
     4861 aattatttta caaaaagcga tggttctatt gtatcaattc cagggactct tttaatttct
     4921 gccatcagca tcatagatga cgttcagaaa acaacaggtt ctgattttaa ggaagccggc
     4981 aatttgatat acgtgctcgg agaaaccttt gatgaacttg gtggttcaag atttctgagt
     5041 atttttaatt tagaaggcgg tagatgtcct gtgccgaatc ctgaaattag tttggcgatt
     5101 atgaggaatc ttcatcaggc aataaagaac aatctgataa tgtcctgtca tgattgctca
     5161 gaaggtggat ttgcgattgc cattgctgaa atgatgatag gtagcggcta tggcgcagaa
     5221 gtatatcttg accggatgcc tgcaacaacc gaagaaactg tatcactttt attcggcgaa
     5281 agtcagggaa ggtttgttgt tgaggttgat gaggcatgcg ttgagaaatt tgaaaaactg
     5341 tttaaagacc ttccttgcaa acaggtcggg agagtgattc ctgattttcg cttgaaggta
     5401 aatcacaggg ataatatgct gcttgatgtt gatggcgaaa tgatgagaca aaggtggtca
     5461 ggagcaattt catggtaaaa gtcattattc ttcgaactgg tggaacaaat tgtgaccttg
     5521 aaacagaatg ggcttgcaaa actgctggag caagtgtttc aagagtccat atttcacaga
     5581 ttacatctgg aagggtcaaa attcttgatt ttgacgtttt gattgttcct ggcggttttt
     5641 cttatgggga tgaccttggc gcaggtaagg ttctggcaaa cgaaataatg ctgaaatgtc
     5701 aggaacagat agaaaaattc agagagaagc agagacctat tattggcata tgcaatggat
     5761 ttcaggtgct ggtgaaggcg aaaatgttac catgctgggg aaaacatagt gccagccttg
     5821 tctggaacga ttctggcaga tttgaggata ggtgggtgca tctcaaagtt gaaaaaagca
     5881 tttctccttt ttttgaaaat ttgccagaaa ttattagatt tcctgtggcg catgctgaag
     5941 ggaaatttgt tgtgaaaact gatagcatat taaaaaagat tgaaaaaaat caacaggttg
     6001 tcctgaggta tgtcgacccg tctggagaaa taaaagggta tccttacaat cccaatggtt
     6061 caattgcagg tattgctggt gtgtgcgatg agaaagggct tgttgttgga atgatgcctc
     6121 atcctgaaag ggcgttactc acactacatt tgcctgattg gttgagattg aggcagcggc
     6181 aggaattttc ttatggatat cagtttttca aaaatgtgat agaatattgc ttgtaaattt
     6241 ttttcagtga aaggagttga aattggtaga aaatagtatt catgctaaag cagaaacact
     6301 tattgaggcg cttccgtata tccaggatta taagggcaag acatttgtta taaaaattgg
     6361 tggcgcgata ttgaggttgc ctgaatcaaa aaagaatatt cttcaggata tcgcttttct
     6421 cagtgctgtg ggaatcaaaa ctgttgttgt gtgtggagca ggtcccacca tcacagaaga
     6481 gattgaaaaa aggggcaaga aggcaaaatt tattgagggt ttgagggtta ctgacagaga
     6541 aacccttgat attgttgtcg aatttctcgg cgccgcaaga gatgaagttg ttgagacatt
     6601 gaaaaatgaa ttcaggacag aagcattttc cttaaaacca gaagacagac accttattgc
     6661 aaaaaaaatt cactggcaga aaggcgacga aatcattgac ctgggatttg taggacaggt
     6721 ggaaaatgtt caggcagatg ttcttgcttc ttttcttgaa aagggtgttg ctgtgttcgc
     6781 tccgattggc atttcactgg atggacagaa gtataacatc aatggggatt cagtttcttc
     6841 tgctgttgcg caatctcttg gcgctgagaa actaattttt gtcaccggtg tcaggggagt
     6901 gatgagaaat ccagataatc ctgatacttt gctatctgtc atcactgcgt cgcagggaga
     6961 aaatctgatt aaagatgaag taatttcggg cgggatgata ccaaaaatca gaggcgcaat
     7021 cgggagtttg aaaggtggtg tgaaaaaggt gcacattatc agtggaaatg tggcgcattc
     7081 aattattctt gaagtgttca cagaacaggg tattggaaca gaaatcatac ttgacgggga
     7141 aaacaatgga tgaaataact gtcaagcaat actacaatga ttatattatt ggttgctatg
     7201 gtaaagaaaa ttttgtgttt gtgaagggta aaggtccatg gttgtgggat atcaatggaa
     7261 aaaagtacct tgattttttc cctggatggg cagtgagtgg acttgggcat tgtccaacaa
     7321 gggttgtgaa agcactgaca aaacaactaa aaaccctgat gcacgtggca aacaattatt
     7381 acattgatgt tcaggcattg tgcgcaaaga aactgattga aatttctttt gatgggaagg
     7441 tatttttttg taatagtggg gctgaagcca atgaggca
//
LOCUS       DAORYU010000051         7454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_7034_length_7454_cov_3.977294, whole genome shotgun sequence.
ACCESSION   DAORYU010000051 DAORYU010000000
VERSION     DAORYU010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7454
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7034_length_7454_cov_3.977294"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PLW07_02710"
     CDS             complement(<1..283)
                     /locus_tag="PLW07_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49465.1"
                     /translation="MRYTIKALAEYIRGNWTEKDIEQWLSMLGLNPVFTEEGNDVLIE
                     IEIPANRGDLLSAIGLIRALSPCGEIEPVYPDTDVKEESSRVFPIETYDV"
     gene            complement(288..1325)
                     /gene="pheS"
                     /locus_tag="PLW07_02715"
     CDS             complement(288..1325)
                     /gene="pheS"
                     /locus_tag="PLW07_02715"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOL49466.1"
                     /translation="MDEKELMLSIRKIVDEAMEQLGKIQDGEGLEKWRIQYFGRKGTF
                     ASMTGRIPQMAVEIRPRAGALINDAKGQLTEAFEQKKTLLTQQGHKRTEHAVNFSLPG
                     LRPETGTLHPVTLVSREIVRIFQGLGFGVHVGPEIETDFYNFEALNMPADHPARDMWD
                     TFYLDPERKFLLRTHTSPVQIRIMEKISVPLRIIAIGKTFRRDDFDASHSPVFHQVEG
                     LMVGHDITFSNLKAVLIFFLKELFGTRVKVRFLPSYFPFTEPSAEISISCVICDGRGC
                     QTCGSTGWLEILGAGMVHPEVFRKSGIRQKNITGFAFGAGLERITMIKYGIDDIRQFY
                     TGDIRFLKQFG"
     gene            complement(1318..1905)
                     /gene="nadD"
                     /locus_tag="PLW07_02720"
     CDS             complement(1318..1905)
                     /gene="nadD"
                     /locus_tag="PLW07_02720"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HOL49467.1"
                     /translation="MAKLEHKIGVFGGSFDPIHMGHLIIAESARQQFRLEKVIFIPSN
                     IPPHRGALVASATRRFEMVKLAIMDNPVFDVSDIEINRAGISYTYETLMLLQQQLRSK
                     IVLIIGWDAFLILPSWYNAEKLAEKFSFIVAPRITENSEMPRFPFPAKYEILEAPRIE
                     ISSTLIRKKIKANQSIRYLVPDKVFHYIMKENIYG"
     assembly_gap    1942..1951
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1952..3141)
                     /locus_tag="PLW07_02725"
     CDS             complement(<1952..3141)
                     /locus_tag="PLW07_02725"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOL49468.1"
                     /translation="MDWKNEINTMAIDARNCSFELACLSSERKNEFLSLLAEILIQKS
                     DKILMFNQKDVKKCQKHNYSQAFIDRLLLTPERIKKMAASLRNIVNLEDPVGKIIWET
                     IRPNGLVIKKVRVPIGVIAIIYESRPDVTIEATSLCIKSGNCVLLRGGKESVFSNSIL
                     VECVKEALEKTGINQNAVNLVKVGGRAGVKYLLSLNESIDLVIPRGGESLIRAVVSHS
                     KIPVIKHYKGVCHIYVDSEADETMALKVCLNAKVQRPGTCNAMETLLVHRDIAHSFLP
                     EMAELFKQHNVEIRGCNETLKIIPWAKPATEQDWAEEYLDLIISIKIVNNVSEAIAHI
                     NRYGTRHSDAIITRNNDTASRFIEEIDSACVYHNASTRFTDGGEFGLGAEIGISTDKI
                     HARGP"
     gene            complement(3141..3971)
                     /gene="proB"
                     /locus_tag="PLW07_02730"
     CDS             complement(3141..3971)
                     /gene="proB"
                     /locus_tag="PLW07_02730"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOL49469.1"
                     /translation="MKKISSEEIEERKKVVKKIKKVVIKIGTNVLTSVGHRLDTSLME
                     HLGQQICWLVESGRQVIVVTSGAIGAGMQILGWEKRPSKIEKLQAAASVGQSRLMRFY
                     ERIFREEGYNVGQVLLTRDIFESEERKNNAFATLETLLYYKIIPVINENDAVASEEIK
                     FGDNDQLSAMVVELIKADALILLSDVDGFYDADPKNCGRVKLISEVSYITESHLSIAK
                     NGNPSELGRGGMVSKLLAIKRVLTIGCTGFIANGKRPWVLRDIFLGKKVGTIFLPEKK
                     "
     gene            complement(3981..4832)
                     /locus_tag="PLW07_02735"
     CDS             complement(3981..4832)
                     /locus_tag="PLW07_02735"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HOL49470.1"
                     /translation="MKMFKSIGIAGLGLLGGSVALAAKSNGLVSEVFGYTRSSDTLQR
                     ACDLGIVDRPFSTFSEMVKEAECVFLSGPISVNLELTKSILEVKPTLLFTDVGSTKKA
                     ITDVVEKLFPYRNCFAGSHPMAGSEKRGIDQARCDLFSGRTVIITPVKNADTQAVETI
                     RNFWERVGAKTVIMSAEKHDTICSYTSHLPHLVAFLLVEMLEGKLDDSDVMSCIGPGF
                     CDTTRIAASDQEIWAEIFASNKENMLLAMKKFQLHLEQIERIIEKNEVEHLKKWIEKV
                     KQMRMSI"
     gene            complement(4841..5605)
                     /locus_tag="PLW07_02740"
     CDS             complement(4841..5605)
                     /locus_tag="PLW07_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HOL49471.1"
                     /translation="MFVWAIIGIFVAITIITNTIKIVPEYERGVVFRLGRLVGPRGPG
                     LFFLIPGIERMIKVSLRTVTFDVTPQEVMTRDNVPIKVNAVVWFRVMDPSKAIVEVEN
                     YHLSTMQIAQTTLRGVLGQMELDEILSERDKINHKLQQIIDEQTDPWGIKVSIVEIKE
                     VELPDTMKRAMARQAEVERERRAKVINAEGEYQASEKLVAAARKLSEVPIAIQLRYLQ
                     TASEIATEKNSTLVFPIPLEMFSVFKQMAEKKADQS"
     gene            complement(5675..7057)
                     /gene="gltA"
                     /locus_tag="PLW07_02745"
     CDS             complement(5675..7057)
                     /gene="gltA"
                     /locus_tag="PLW07_02745"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HOL49472.1"
                     /translation="MRQNRHHPQKQKIEDRTKNFNEVSHGLTEQDAIEEAKRCLQCKK
                     PLCVAGCPVEVDIPGFIKKIAEKDYDAAIKIIKEKNVLPGICGRVCPQETQCEGVCIH
                     GKKSEPVAIGLLERFAADMEKEKNIPIIKEKKTTPVAVIGSGPAGLTCASELARHGYP
                     VTIFESLHLPGGVLTYGIPEFRLPKKIVKDEIEFVKKLGVEILTDAIIGKTLTIDDLR
                     NMGYKAIFIGVGAGLPSFMGIPGENLLGVYSANEFLTRINLMKAYKFPESDTPVQVGS
                     RLAVIGGGNVALDAARCALRLGAKVSVLYRRTENEMPARREEYENAKEEGIEFHFLTL
                     PIRFVGDENGYLKGIEVQKMQLGEPDESGRRRPVPIPGSETVMDFETAVVAIGQRPNP
                     LLTSTTPELKLGKSGIIVADPETGQTNIKDIFAGGDITTGAATVISAMGAGRKAAQSI
                     MKYLETIEKT"
     gene            complement(7068..>7454)
                     /locus_tag="PLW07_02750"
     CDS             complement(7068..>7454)
                     /locus_tag="PLW07_02750"
                     /inference="COORDINATES: protein motif:HMM:NF014907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49473.1"
                     /translation="LGSFHHGKTAALPEYKISKQNIPFIDIYDLQRSIKSLMWKNVGI
                     ERHQDSLTEAINRLDTWGKYIFLKEFSQQTGWEVQNMFILSSVMAHAALKRQESRGAH
                     FRKDFPETDDKNWKKRQTFSKNDFSL"
BASE COUNT         1993 a         1768 c         1264 g         2419 t
ORIGIN      
        1 caacatcgta tgtttcaatc ggaaaaactc ttgaggattc ttcttttaca tctgtatcag
       61 gatatacagg ttctatctca ccacagggag aaagtgccct gattaaacca atagcactta
      121 aaaggtcgcc cctgtttgca ggaatttcaa tttcaataag cacatcattg ccttcttcag
      181 taaatactgg atttaacccg agcatactga gccattgctc aatatctttc tctgtccagt
      241 tccccctgat gtattcagcg agtgctttga tagtgtatct cattgtatca tccgaattgc
      301 ttgaggaaac gtatgtcgcc tgtgtaaaac tgtcttatat catctatccc atacttaatc
      361 atggttattc gttcaagtcc tgcgccaaaa gcaaaacctg tgatattttt ctgtcgaatt
      421 ccggatttgc ggaacacctc aggatgaacc atacctgcgc caagaatctc aagccatcca
      481 gtagaaccac aggtctggca tcccctgcca tcgcagatta cacaggaaat tgaaatctct
      541 gcgcttggct cagtgaaagg gaagtaagaa gggagaaacc tcaccttcac cctggttcca
      601 aacagttctt tcaaaaagaa tatcaacacc gctttcaggt tgctgaaagt gatgtcatgt
      661 cccaccatca atccttccac ctgatgaaaa accggtgaat gggatgcatc gaaatcatcc
      721 ctcctgaatg tttttccaat ggcaataatt cttaacggca cagaaatttt ttccattatc
      781 ctgatctgta cagggcttgt atgcgttctc aaaagaaatt tcctttcagg gtcaagataa
      841 aaagtatccc acatatccct ggcaggatgg tccgcaggca tattcaatgc ttcaaagttg
      901 taaaaatcag tctctatctc aggtccaacg tgaacaccaa aaccgagtcc ctgaaaaatc
      961 ctgacaatct cccttgaaac aagtgtaaca ggatgaagtg tacctgtttc tggtctcaat
     1021 cctggaaggc tgaagtttac ggcatgttca gtccttttgt gtccctgttg agtcaaaagt
     1081 gttttctttt gttcaaatgc ctctgttaat tgcccttttg catcattaat caatgctcca
     1141 gcgcgtggtc ttatttcaac tgccatctgt ggaatcctgc cagtcatgga ggcaaaagtc
     1201 cctttccttc caaaatattg aatcctccat ttctcaagac cttcaccatc ctggatttta
     1261 cccagttgct ccatcgcctc gtccacaatc ttcctgatgc tcaacatcaa ttctttttca
     1321 tccataaatg ttttccttca ttatgtagtg aaaaacttta tcaggaacaa gatatctgat
     1381 tgattgattt gccttaattt tttttcttat cagcgttgaa gaaatttcaa ttcttggcgc
     1441 ttcaaggatt tcatacttcg caggaaacgg aaaacgtggc atctcgctat tctcagtaat
     1501 tcgcggagcc acaatgaaag aaaacttttc tgcaagtttt tctgcgttat accatgacgg
     1561 taaaatcaaa aacgcatccc atcctataat cagcacaatt ttactgcgca actgttgctg
     1621 taaaagcatc aatgtttcat aggtgtagga aataccagcc ctgttaatct cgatatccga
     1681 cacatcaaaa actggattat ccataatagc aagctttacc atctcaaacc gccgggttgc
     1741 agaagcgaca agggctcctc tgtgaggagg gatatttgaa ggaataaaaa taaccttctc
     1801 aagacgaaac tgctgtcgcg ctgactcggc aataataaga tgtcccatat gaattggatc
     1861 aaaacttcca ccaaatacac ctattttatg ttctaatttg gccatttccg taaatcaggt
     1921 atttgtaggt tgttaattct tnnnnnnnnn nggacctctt gcgtgaattt tgtcagtact
     1981 gattccaatt tctgcgccaa gcccaaattc tccgccatcg gtaaatcttg ttgatgcgtt
     2041 gtggtagacg cacgcagaat caatttcttc tataaacctg ctcgctgtat cgttatttct
     2101 ggtgataatt gcgtctgaat gtcgtgttcc atatctgttg atatgagcaa tcgcttctga
     2161 tacattgttg actattttaa tggaaataat caggtcaagg tattcctctg cccaatcctg
     2221 ttctgtcgct ggttttgccc aggggataat tttcagtgtt tcattacaac cgcgaatttc
     2281 aacattatgt tgtttgaaca attctgccat ttcaggaaga aatgaatgtg caatatctct
     2341 atgaacaaga agcgtttcca tagcgttgca tgttcctggt ctctgcactt ttgcattcag
     2401 gcataccttc aatgccattg tttcatctgc ctcgctgtca acataaatgt ggcatacacc
     2461 tttgtaatgt ttaatgacgg ggattttgct gtgtgaaaca actgccctga tcaaagattc
     2521 cccgccccgc ggtatcacca ggtcaatgct ttcattcaaa gacagaaggt attttactcc
     2581 tgcccttcct cccactttta caagatttac agcattttga ttaatacctg ttttctccag
     2641 cgcttccttt acgcattcca caagaattga atttgaaaaa accgattctt tgccaccgcg
     2701 caacagaaca caattaccgg attttataca cagagaggtt gcctctatgg ttacatcagg
     2761 ccgtgattcg taaataattg ctatcacacc aatgggcact ctcacttttt taatgacaag
     2821 tccattggga cgaatcgttt cccatatgat tttaccaaca gggtcttcaa gatttacaat
     2881 attgcgcagc gatgctgcca tcttttttat tctctctgga gtcagaagca atctgtcaat
     2941 gaatgcctgc gaataattgt gtttctgaca ttttttcaca tccttttgat tgaacatcaa
     3001 aattttatca ctcttctgta tcagtatctc tgccagaagt gaaagaaatt cattttttct
     3061 ttctgaagaa agacatgcaa gttcaaatga gcaattgcga gcatcaatcg ccatcgtatt
     3121 gatttcattt ttccagtcca ttattttttc tccggcaaaa aaatcgttcc aaccttttta
     3181 ccaaggaaaa tatctctgag cacccatggt ctcttaccat ttgcaataaa acctgtacac
     3241 ccgattgtca acactctctt gattgccagc agttttgaaa ccattccacc tcttccaagt
     3301 tctgatgggt taccgttttt tgcgatagac agatgagatt ctgtaatgta actgacttct
     3361 gaaatcaatt tcacccttcc gcaatttttt gggtcagcgt cataaaaacc atccacatca
     3421 gacaaaagaa tcaatgcatc ggctttaatc aattccacca ccattgcaga aagctggtca
     3481 ttgtctccaa attttatttc ctcagaagca accgcatcgt tttcattgat aaccggaata
     3541 attttatagt aaagcagtgt ctcaagcgtt gcaaaggcat tgttttttct ctcttcggac
     3601 tcaaaaatat cccgcgtgag tagcacctgc ccgacattgt atccttcctc gcgaaaaatc
     3661 ctttcgtaaa atctcatcaa tctgctctgt ccaacgcttg ccgcagcctg aagtttttca
     3721 atttttgaag gtcttttttc ccatccaagt atctgcattc ctgcgccaat ggctccagat
     3781 gttactacaa taacctgtct tccgctttca acaagccagc atatctgttg ccccagatgt
     3841 tccatcagag aagtgtccag tctgtgtccc acggatgtaa gaacatttgt tccaattttt
     3901 atcacaacct tcttgatttt cttgacaacc ttttttcttt cttctatttc ttcgctggat
     3961 atttttttca tttttctgac tcaaatactc attctcattt gcttgacttt ttcaatccat
     4021 tttttcaaat gttcaacctc gtttttttca ataatccgtt caatctgttc aagatgcaat
     4081 tgaaattttt tcatagccag aagcatgttt tctttattgc tggcgaaaat ttcagcccat
     4141 atttcctggt cgcttgcagc aatcctcgtt gtatcgcaaa atcctgggcc tatgcatgac
     4201 atcacatcag aatcatccag ttttccttca agcatttcca caagcaaaaa agccacaagg
     4261 tgaggaagat gacttgtata agaacatatt gtatcgtgtt tttcggcgct catgatgact
     4321 gtttttgcac caactctctc ccagaaattc ctgatggttt caactgcctg agtgtcagca
     4381 ttcttcaccg gcgtgatgat aacggttctg ccagaaaaca ggtcacatct cgcctggtca
     4441 attccccttt tctcagaacc tgccatagga tgggaaccag cgaaacaatt tctgtacgga
     4501 aataatttct caacaacatc tgtgattgct ttttttgtgc tgccaacatc tgtaaataac
     4561 agtgttggtt taacttccag aatacttttt gtaagctcca gattcactga aatcgggcca
     4621 gaaagaaaaa cacattctgc ttccttcacc atttccgaaa aagttgaaaa cggcctgtca
     4681 acgattccaa ggtcgcatgc tcgttgaagt gtatcagaac ttcgtgtgta gccaaacact
     4741 tcagacacca gtccattgct ttttgctgca agagccacag aaccaccaag caaaccaagt
     4801 ccggcaatac caatactctt gaacattttc attactggta tcagctctga tctgctttct
     4861 tctcagccat ctgtttgaaa acagaaaaca tttcgagagg aataggaaac accagtgttg
     4921 aattcttctc tgtcgctatc tctgacgctg tttgcagata tctgagctga attgctattg
     4981 gaacttcaga aagttttctc gcagctgcta caagtttttc acttgcctga tattctccct
     5041 cagcattgat aactttggct cttctttccc tttcaacttc tgcctgtcga gccattgccc
     5101 gtttcattgt atcaggcaat tcaacttcct taatttccac aatactcacc ttgatacccc
     5161 atgggtctgt ttgttcatca ataatctgct ggagtttgtg attgattttg tccctttctg
     5221 aaagtatctc atccagttcc atctgtccca gaacaccgcg aagtgttgtt tgcgcaattt
     5281 gcattgtgga aagatggtaa ttttcaactt caacgattgc ttttgatggg tccataaccc
     5341 tgaaccacac cactgcatta accttgatag gtacattgtc tcttgtcatc acttcctggg
     5401 gcgtaacatc aaaggtcact gttctcaaac tcaccttaat cattctttca atccctggaa
     5461 tcagaaaaaa taatcctggt ccccttggtc ccactaatcg accaagccga aataccacac
     5521 ccctttcgta ttcaggaaca attttgatgg tattggtaat aattgttatt gccacgaata
     5581 tcccgattat cgcccataca aacattttac ctccctgtta aggaaaatat tttgattaaa
     5641 tgttaacttt tttcaccagc gatgtcaaag aatatcaggt cttttcaatt gtttcaaggt
     5701 atttcataat cgattgcgct gcttttctac ctgcgcccat tgcactaatc acggttgctg
     5761 ccccggttgt gatatcgccg cctgcaaaaa tatccttgat gtttgtctga cctgtttcag
     5821 ggtcagcaac aataatacct gattttccta atttcagttc tggagttgtg gatgttaaaa
     5881 gaggatttgg tctttgccct atggcaacaa ccgctgtttc aaaatccata acagtttcag
     5941 aaccagggat tggaaccggt cgtctccttc cactttcatc tggttccccc agttgcatct
     6001 tttgaacttc tattccttta agataaccgt tttcatcgcc aacaaatcgt atgggaagag
     6061 taagaaaatg aaactcaatg ccctcttctt ttgcattttc gtattcttcc cttcttgcag
     6121 gcatttcatt ttcagttctt ctgtaaagca cactcacctt tgcaccaagc cttaatgcgc
     6181 atctggcggc atcaagagcc acattgccac cacctatcac agcaagtctg cttccaacct
     6241 gaacaggcgt atctgattcc gggaatttat acgccttcat taaattgatt cttgtgagga
     6301 actcattggc agaataaacc ccgagaagat tttcaccagg aatgcccata aatgatggca
     6361 atccagcacc aacaccaatg aaaatcgctt tatatcccat atttctcaaa tcatcaatcg
     6421 tcaatgtttt accgataata gcatcagtaa gaatttcaac accgagtttt tttacaaatt
     6481 caatctcgtc tttcacgatt tttttgggca atctaaattc aggaataccg tatgtcagaa
     6541 cacctcctgg tagatgcaaa gattcaaaaa ttgtcacagg atatccgtgt cttgcgagtt
     6601 ctgaagcaca cgttaaacct gctggaccac taccaataac agcaacaggc gttgtttttt
     6661 tctcttttat tatgggaata tttttttctt tttccatatc agcagcaaat ctttcaagaa
     6721 gtccgatggc gactggttct gattttttcc cgtggatgca cactccctca cactgggttt
     6781 cctgcggaca tacccttcca catataccag gaagtacatt cttctcttta atgattttta
     6841 ttgctgcatc ataatctttc tctgcaattt ttttgataaa tccagggata tcaacttcca
     6901 caggacaacc agccacacat aatggttttt tacattgaag acatcttttg gcttcttcaa
     6961 tggcatcctg ctccgttaat ccatggctga cttcattaaa atttttggtt ctatcttcaa
     7021 tcttttgctt ctgcggatgg tgtctattct gcctcatctt gttcctttca tagagaaaaa
     7081 tcgttctttg aaaatgtctg tcgcttcttc caatttttgt cgtctgtctc cggaaaatcc
     7141 tttctgaaat gtgcgcccct gctttcctgt cttttcaatg ccgcatgcgc catgacagaa
     7201 gaaagaataa acatattttg aacctcccat cctgtctgtt gcgaaaattc tttcaaaaat
     7261 atgtactttc cccatgtatc aagtcggttt atggcttcag ttaaagaatc ctggtgtctt
     7321 tctattccca catttttcca catcaaactc ttaatggacc tctgtaagtc ataaatgtca
     7381 ataaatggaa tattttgctt actgattttg tactccggca gggcagcggt tttgccatga
     7441 tgaaatgaac ctaa
//
LOCUS       DAORYU010000052         7310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_7198_length_7310_cov_4.718815, whole genome shotgun sequence.
ACCESSION   DAORYU010000052 DAORYU010000000
VERSION     DAORYU010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7310
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7198_length_7310_cov_4.718815"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="PLW07_02755"
     CDS             <1..775
                     /locus_tag="PLW07_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49474.1"
                     /translation="FLLLDPTSEVIPYPELPPGDQNKTVLIPTENETILIKTYTARPE
                     ENKKEREIFVSLDPNGDIIANVTVLPTGIFAAGLRNSFRYLSNEERKRELSRSINSLV
                     PNSTLKEFKITGIESLDTPLSQTYTFISKKYAIPIKEKLLLKPALLERLENTELVSLE
                     TRIFPIRFPYKSMNKDEILFSLPPGYDIDVYPEDIKFENKFGSYSIKFEIKDHKLFYQ
                     RILCLNVLEIGAEQYLEFKNFYQQISYYDSLPVILKKNQ"
     gene            875..1990
                     /locus_tag="PLW07_02760"
     CDS             875..1990
                     /locus_tag="PLW07_02760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOL49475.1"
                     /translation="MDQTNTTEINLKKLLSVMESINASDIHLQAGCHIAYRIKGEIEK
                     QTDWPPLSEEQLRKIVYEVLTERDKKIFEEKGTVDTAISFPGIGRFRMSVFLQRGSIA
                     LAARRISNKIPDFEFLHLPEAAKNITNFPNGLVLVTGPTGCGKSSTLAAIINGINKIR
                     KCHIMCIEDPIEYLYKNELAIINQREIGIDVPSFKEALKYVVRQDPDVILVGEIRDRD
                     TVDFALQSAETGHLVFGTLHSSNVSQTINRILNFYPHSEHPSVRRALAGHLKAVMSQM
                     LLPSCKEGILMVPAVELMFMNSTIARLIVEEQESKIVKAIKAARTEGMQDFEQSLYEL
                     ITNGFITKEIGLEHAENPHSLDMKLRGISLTEEGGIV"
     gene            1992..2783
                     /locus_tag="PLW07_02765"
     CDS             1992..2783
                     /locus_tag="PLW07_02765"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOL49476.1"
                     /translation="MKRFLLLVLISLSYFIACSGSERVLTFVIFSDCHLKDEKTISEQ
                     LLQNIVQKINSDEQDAEFAVCLGDIVGLPGDAKFQRYLTAVEKYLAITSGLRVPIYTV
                     PGNHDLEQGNEARKTFEKKIGPLFFSVEKNGYQLLFLNSETLNETQLEWLNTQFKKPY
                     QSRIIFMHRPLFAVFTGHAFDTKTTVFLKQLFESHNVIAIFSGHEHLFYQKKYGNILQ
                     VISGGAGGTLVPAPEDGKSIFHYCRVKVSKSDIIVEPVQVDIKIF"
     gene            2794..4056
                     /locus_tag="PLW07_02770"
     CDS             2794..4056
                     /locus_tag="PLW07_02770"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOL49477.1"
                     /translation="MDWQTINLGNGASVIYKQRHDCFSVSMGIIVGSGVRYESLEHSG
                     ISHFVEHLLFKGTKKRSANQLKEAIEGKGGSFNAFTGEEIVCYYIKILERHLETGLDI
                     LSDMITNPLFDPEEINKERNVILEEINMYMDMPARYVFDLLDAAMFENHPLGTSGLGT
                     PETLKKIDRQSLIDYVRRFHTGKNLTISVAGQFEEKNLINLVERFFGHIHDGEKGQFV
                     PFENNQGHARYKIKTRETEQCHFCIGFYSYPREDEKRFPLAVANVILGGNMSSRLFNE
                     VREKRALVYDIRSHIKSYYDTGAAIISAGLTPANLLECVKIIKDQLNCIVEVKVSDEE
                     LMRAKEYLVSQFLMGLEDTMEYMLWIGEQKATRKRMLSIEEIENKIKDVTAKDVQNVC
                     RDIFSKNACFAMIGPQDRTEEILKILEG"
     gene            4058..4375
                     /locus_tag="PLW07_02775"
     CDS             4058..4375
                     /locus_tag="PLW07_02775"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOL49478.1"
                     /translation="MIKKDIIEEISKQTGLSKPVVRMVVEKFILQVRNSLLKKERIEL
                     RNLGVFNVKRMKAKKGRNLKTGEEIPVPERWKVTFKPSKIFMKLNTEKPEQTLPFDTS
                     NNH"
     gene            4467..6008
                     /locus_tag="PLW07_02780"
     CDS             4467..6008
                     /locus_tag="PLW07_02780"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOL49479.1"
                     /translation="MKKLIVITFFSVVFFTTNHVLLAADTIQIAKNEKSLVPVVISEN
                     ASENMKKLANELAEYLNKITGASFEIKTGDGSTGIIVGTVAQFPDADIAKKLEIRNIF
                     DGKEAYAIKSEKKRLVLIGATEQGTSHAVFRFLELVGCRWFFPAKEWEIVPRVKNLAL
                     RVEEYSRPNFLSRRIWYGFGFFDRHTGKVAEDYNAWMRHNRMGGSLKVSFGHAWQTII
                     ADNKAIFEQHPEYLALSNGKRQGNKFCVSNPEVRKIVINWALNYFEKHPDADMVSVEP
                     SDGLGHCECEKCLEMGSVSDRVFYLANEVAKAIREKYPEKLVGLYAYADHSEPPSFTL
                     EPNVWIQRTAGFTYGKYSSEELMQIWPKYCQNMGYYEYLSVYLWDWDMVPGGRAANIS
                     YLKKQIPLYYKNAGRTLDCESGNNWGPHGLGYYITSKLMWDVNTNVDALCEDFYQKAF
                     GPAAGVMKRYYERLDPGNNPLRCENLIGEALRDLDEASSLTKNRPDIQARLDHLKQYM
                     HYVRL"
     gene            6054..7013
                     /locus_tag="PLW07_02785"
     CDS             6054..7013
                     /locus_tag="PLW07_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49480.1"
                     /translation="MLEVLTWTYRTRYTYMNHWEAIHQYLAPKAAKDFNEPAWTSFYQ
                     DKSSSKPWADNTPVTHEETEASFQKDLQRFPVVKVNEKTFSENLVPANFPFQSSAGVS
                     QAYQGKVSYAMYSRNGEDLEFTITTGIIAAYRNRKHAEYILTDASGKTIVSEKLPLDG
                     NQHQIKVSVPTLGLYFLAIDDSSAGFRFTTNPQMPCVIIQKKGVSLHHMGHMQQLYFY
                     VPKGTKQLQYFIDNPTKHVFYGPDGKQIKEIEADDVFVALEVPEGLDGKVWSVKYLAL
                     GQLWFFNIPSYLAPSPDNLLVPKEIAIQDKLLSQEAPPTGVQK"
     gene            7010..>7310
                     /locus_tag="PLW07_02790"
     CDS             7010..>7310
                     /locus_tag="PLW07_02790"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl-hydrolase"
                     /protein_id="HOL49481.1"
                     /translation="MRYIKKDKTIYTGKNLNYISFPTGGIGAGMICLDGNGSFSHISL
                     RHKPNLQKYASYMFAAFAVKKKVPRILEGHVPEWKIFSLPEAGNGLGETHFGLPRC"
BASE COUNT         2568 a         1284 c         1478 g         1980 t
ORIGIN      
        1 atttcttttg cttgatccaa cctcagaagt tattccatat ccagagttgc ccccaggtga
       61 ccagaataaa acagttctaa taccaacgga aaatgagaca attcttatca aaacctatac
      121 cgcaaggcct gaagagaata aaaaagagcg tgaaatcttt gtttcgctgg atcccaatgg
      181 ggacattatc gccaatgtta ctgttctgcc aacaggaatt tttgcggcag gattaaggaa
      241 tagcttcaga tacctctcca atgaagaaag aaaacgagaa ctttcaagaa gtataaattc
      301 attagtacca aattcaacct tgaaagaatt caaaattacg ggcattgaat ctctcgacac
      361 accactgtcc caaacttata cattcatttc aaaaaaatat gcgataccaa tcaaggaaaa
      421 attgttgtta aaaccagctc tgcttgaaag actcgaaaat actgaactgg tgagtctgga
      481 gacgagaatc ttcccaataa gatttcctta caaaagtatg aataaggacg aaatcttatt
      541 ttcacttcca ccgggatatg atattgatgt gtatccagaa gatataaagt ttgaaaataa
      601 attcggatcg tattccatca agtttgaaat caaggatcat aagttatttt atcaaaggat
      661 actatgcctg aatgtccttg aaattggtgc tgaacaatat ctcgaattca agaatttcta
      721 tcagcaaatt tcgtactatg atagtctgcc agtaatatta aagaaaaacc aataataagt
      781 aggaaatttc gaaaaaatca cttgtgaaaa aaaacttttg tgatattatc atttaatgtt
      841 aataagataa caaacttttc catggaggga tggaatggac cagacaaata caacagaaat
      901 caatttaaag aagctgctat cagtgatgga gtcgataaat gcatctgata ttcacctgca
      961 agcgggttgc catatcgctt acaggataaa aggtgaaata gaaaaacaaa cggattggcc
     1021 gccactttct gaggaacaat taagaaaaat tgtttacgaa gtccttacag aaagagacaa
     1081 aaaaattttt gaagaaaagg gtacagttga cacagcaatc agttttcccg gcataggaag
     1141 atttagaatg agtgtatttc ttcaaagggg ctcgatcgca cttgctgcaa gaagaatttc
     1201 caacaaaatt cctgattttg aatttttgca cttaccagag gctgcaaaaa acataacaaa
     1261 ttttcctaat ggtctggtcc ttgtcacagg tccaaccgga tgtggaaaat caagtactct
     1321 tgccgcaatt attaatggta tcaacaaaat tagaaaatgt catattatgt gtatcgaaga
     1381 tcccatagaa tatctctaca aaaatgagct cgctattatc aatcaaagag aaattggcat
     1441 cgatgttcca agctttaaag aagctttgaa gtatgttgtg agacaggatc cggatgtgat
     1501 actggttggt gaaataagag atagggatac agtggacttc gcacttcaat cagctgaaac
     1561 aggtcatctt gttttcggca ctctccacag ttcaaacgtc agccagacaa ttaatagaat
     1621 cttgaacttt tatcctcatt cagaacaccc ctcagttcgc agagcactcg cagggcatct
     1681 taaggcagtt atgtcacaga tgcttttacc atcctgcaaa gaaggaatac tgatggtacc
     1741 agctgttgaa ttaatgttca tgaactcaac aattgcaagg ctcatcgtag aagaacagga
     1801 atcaaaaatt gtcaaggcaa tcaaagcggc aagaactgaa ggaatgcagg attttgaaca
     1861 atcattatat gagttgataa caaacggatt tataacaaaa gaaattggac ttgagcatgc
     1921 tgaaaatcct cacagccttg atatgaaatt aagaggaatt tcattgaccg aagaaggtgg
     1981 catcgtataa catgaaaagg tttctactgc tggttttgat atctttatca tatttcattg
     2041 cgtgttctgg aagtgagaga gtactgactt ttgtaatatt cagtgactgc catctgaaag
     2101 acgagaaaac tataagtgaa cagttacttc aaaatattgt gcaaaaaatt aactcagatg
     2161 aacaggacgc tgaatttgct gtctgtcttg gtgacattgt ggggctccca ggagatgcga
     2221 aatttcaacg atacttaacc gcagtagaaa aatatctggc aattacatcg ggtttaagag
     2281 tacctatcta tacagtccca ggaaatcatg accttgaaca gggtaatgaa gcgaggaaaa
     2341 cctttgagaa aaaaatcggt ccattgtttt tcagtgttga aaaaaatgga tatcagctgc
     2401 tctttttaaa tagcgaaaca ttaaatgaaa ctcaattgga atggttgaat acacagttta
     2461 aaaaaccata tcaatccaga attattttca tgcacagacc actgtttgct gtctttacag
     2521 gacatgcgtt tgatacaaaa acaacggttt tcttaaaaca attgtttgaa agtcataatg
     2581 tcatagcaat tttttcaggc catgaacatc ttttttatca aaaaaaatat ggtaatattt
     2641 tacaagttat ttcaggtgga gcaggtggca ctcttgtccc tgcaccagaa gatggcaaaa
     2701 gcatttttca ctattgcagg gtgaaagttt caaaaagcga catcattgtg gaacccgtgc
     2761 aagtggatat caagatattc taacggagac gatatggact ggcaaaccat caatcttggt
     2821 aatggtgcat ctgtaattta caaacaacga cacgattgtt tttcggtctc aatgggaatc
     2881 attgttggtt ctggtgtgag atacgaatct ttggaacatt caggcatatc ccactttgtt
     2941 gagcatcttt tgtttaaggg aaccaaaaaa agatcggcaa accaattgaa agaagccata
     3001 gagggaaaag gtggaagttt taatgcattt actggagaag aaatcgtatg ttattacata
     3061 aaaatccttg agcgtcatct tgaaacaggt cttgacattc tttcggatat gatcaccaac
     3121 ccattatttg atcccgaaga aatcaataaa gaaagaaatg ttattcttga ggaaatcaat
     3181 atgtacatgg atatgccagc gcggtatgtt tttgaccttc tcgatgcagc gatgtttgaa
     3241 aatcatcctc ttggtacttc tgggcttggc acgcctgaaa ccctgaaaaa aatcgacaga
     3301 cagtccttga ttgactatgt aagaagattt catacaggaa aaaatctgac gattagcgtc
     3361 gcaggacaat ttgaggaaaa aaatctgatt aatcttgtag aaagattttt tgggcacata
     3421 catgatggcg aaaagggtca atttgtccca tttgaaaaca atcagggaca tgcaagatac
     3481 aaaataaaaa ccagagaaac agaacaatgc cacttctgca taggttttta ctcctatccg
     3541 cgagaggatg aaaaacgctt tcctcttgct gtggcaaatg tgatccttgg tggtaatatg
     3601 agtagcagac tcttcaatga agttagagaa aaaagagctc tggtatatga catcagaagt
     3661 catataaaga gctattatga tacaggcgct gccattatat cagcaggact gactccggca
     3721 aacctgcttg aatgtgttaa aatcataaaa gaccaactga actgcattgt ggaggtaaag
     3781 gtttcagatg aggaactgat gagagcaaaa gaatatcttg tttcacaatt cttaatgggg
     3841 ctcgaagata caatggaata tatgctgtgg ataggtgaac aaaaagcaac cagaaagaga
     3901 atgctttcca tagaagaaat tgaaaacaaa atcaaggatg tcactgcaaa agacgttcaa
     3961 aacgtgtgca gggatatatt tagtaaaaat gcgtgctttg ctatgattgg tccccaagat
     4021 agaacagaag aaatcctaaa aattctggag ggataatatg atcaaaaaag atatcataga
     4081 agagatatca aaacaaaccg gcctgagcaa accagttgtt agaatggttg tagaaaaatt
     4141 tatattgcag gtcaggaatt cacttctcaa aaaagaaaga attgaactga gaaacctggg
     4201 tgtgttcaac gtgaaaagaa tgaaagcaaa aaagggtcgt aatctgaaaa caggcgaaga
     4261 gattcctgta cctgaaagat ggaaagtcac attcaaacct tcaaaaattt ttatgaaact
     4321 taacacagaa aaacctgaac agaccctgcc ttttgataca tctaataacc actgaaacat
     4381 taatttcaac tctccaaacc attttcatat cttaagtgtg ataaaatatt aaaaatccgt
     4441 caacacttaa ccaaaagttg catatgatga aaaaacttat cgtaataact ttcttttctg
     4501 ttgtattttt tacgacaaat catgtattgc tcgcagctga caccatccaa attgcaaaaa
     4561 atgaaaagtc gcttgtaccg gtggtgattt cagagaatgc gagcgaaaat atgaaaaaac
     4621 ttgccaatga actggctgaa tacctaaata aaatcactgg agcaagcttt gaaataaaaa
     4681 caggagatgg aagcactggc atcatagtag gcacagtcgc acaatttcca gatgctgata
     4741 ttgcaaagaa acttgagata cgaaatatct ttgatgggaa agaagcatac gcaatcaaaa
     4801 gtgagaaaaa acggcttgtt ctaataggtg ccactgaaca gggaacttct cacgctgtat
     4861 ttcgtttcct ggaattggtt ggttgcagat ggtttttccc tgctaaagaa tgggaaattg
     4921 ttcccagagt aaaaaattta gccctgaggg ttgaagaata tagcagaccc aatttccttt
     4981 caagacggat atggtacgga ttcgggtttt ttgatagaca cactggcaaa gtcgcagaag
     5041 attacaatgc atggatgaga cataatagaa tgggagggtc attaaaagtg agtttcggcc
     5101 atgcatggca gacaattatt gctgacaata aagcaatttt cgagcaacat cctgaatatc
     5161 ttgccctatc aaatgggaaa aggcagggaa acaagttttg tgttagcaat cctgaagtta
     5221 gaaaaattgt gattaactgg gctttaaatt atttcgagaa gcatccagat gctgacatgg
     5281 tatcagttga gccttcagac ggactgggac actgtgaatg tgaaaaatgc ctcgaaatgg
     5341 gctcggtttc agacagggtt ttttatctgg caaatgaggt cgcaaaagca attagagaaa
     5401 aatatcctga aaaactggtg ggactctatg cctatgcaga tcattctgaa ccaccatctt
     5461 ttacacttga accaaatgtg tggattcaaa gaacagcagg ttttacctac ggcaagtatt
     5521 catcagaaga actgatgcaa atatggccga aatactgtca gaacatgggt tattatgaat
     5581 atctttctgt ttatctgtgg gactgggaca tggttcctgg aggtagagct gcaaacatat
     5641 catatctaaa gaaacagatc ccactttatt ataaaaatgc cggcaggact cttgattgcg
     5701 agagtggtaa taactgggga ccccatggtc tgggatatta tattacaagc aaattaatgt
     5761 gggatgtcaa cacaaatgtg gatgcattat gcgaagattt ttatcaaaaa gcttttggac
     5821 cagcagctgg tgtcatgaag agatattatg aaagattaga ccctggaaat aatcctttaa
     5881 gatgcgaaaa cttaattgga gaagcattgc gtgacttgga tgaagcaagt tctcttacaa
     5941 aaaacagacc agatattcag gcaaggcttg atcacctgaa acaatacatg cactatgtga
     6001 gattatagtg ggactacaca catacaaagg acaaagataa aaagaaagac ctaatgcttg
     6061 aagttcttac atggacatat agaacccgtt atacgtacat gaatcactgg gaagcaatac
     6121 atcagtatct tgctccaaaa gccgccaagg atttcaatga accagcatgg acttccttct
     6181 accaggataa aagttcatca aaaccctggg ctgacaatac tccagtgact cacgaagaaa
     6241 ccgaagcatc atttcaaaag gatttgcaac gatttccagt ggtaaaggta aatgagaaaa
     6301 ctttttctga aaatctcgtg ccggctaact ttccttttca aagttcagct ggagtgagtc
     6361 aggcgtatca gggtaaggtt tcgtacgcaa tgtacagcag aaatggagaa gaccttgaat
     6421 ttacgataac gacaggtatt atcgcagcat atcgaaatcg aaaacatgct gaatacattc
     6481 ttacagacgc ttcaggaaaa acaattgtta gtgaaaaact tcccctcgat ggaaatcaac
     6541 accagataaa agtttctgta cccactttag gtctttattt ccttgcaatt gatgattcca
     6601 gtgcaggatt tagatttaca acaaatccgc aaatgccatg tgtcataatt caaaaaaagg
     6661 gcgtatcatt gcaccacatg ggacatatgc aacagctata tttttacgtt ccaaagggaa
     6721 caaagcaact tcagtatttt attgacaatc caacaaaaca tgtattctat ggaccagatg
     6781 gaaagcaaat taaagaaata gaggcagacg atgtatttgt ggctctcgaa gttcctgagg
     6841 gtcttgatgg gaaggtctgg agtgtaaaat atctcgccct tggacaatta tggtttttca
     6901 atataccttc ttatcttgct ccttcgcctg acaacctttt ggtacccaaa gaaattgcaa
     6961 tacaggataa actgctatca caggaagcac caccaacagg agttcagaaa tgagatacat
     7021 taaaaaagat aaaacaattt atacagggaa aaatcttaac tacatttcgt ttccaacggg
     7081 aggaattggt gccggaatga tttgcctcga tggaaatggc agtttttctc atatttcatt
     7141 aagacataag ccgaatctac aaaagtatgc atcatatatg tttgcggcat tcgctgtaaa
     7201 aaagaaagtc ccaagaatac ttgaaggtca tgttcctgag tggaaaattt tttcacttcc
     7261 agaggcaggg aatggtcttg gagaaacaca tttcggactt cccaggtgta
//
LOCUS       DAORYU010000053         7213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_7319_length_7213_cov_7.512154, whole genome shotgun sequence.
ACCESSION   DAORYU010000053 DAORYU010000000
VERSION     DAORYU010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7213
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7319_length_7213_cov_7.512154"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1894)
                     /locus_tag="PLW07_02795"
     CDS             complement(<1..1894)
                     /locus_tag="PLW07_02795"
                     /inference="COORDINATES: protein motif:HMM:NF016944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HOL49482.1"
                     /translation="MKNTNQTEISSIIEKAKKCGLDASLLYECVVELAEEGLLTASCI
                     NAAAGILLSDLGLPEYFFKGLSKDSLKTVLKTIASNLEKEGNFYVLRSEVSEVPFTVE
                     GGVQVRIATAENRDRMEKILNPVMSGNRIEYYYGKKHNYYTYIIRPEKCKELKDIKPG
                     QSPFAFNEIVSGLPVPEVTKKRYEEFLKKVEKSFVPIIFVSKSKNTGEIRIMFKEDFS
                     SSVLPTVRKMLEEIGITLNRAYWETYRGKTGRIESICSLYVEGGISSEKMQTTINRLH
                     LLLAIQPSGLDELYTGGQLSFFEYLFAVVASVFVHTFIHKTDPVDRNIMESFSNKEMK
                     DAFARRVFESNRAEYSRSVITSAIKQNPDFVKRIFAIFDKRFNPAQPEKNFEKILHQI
                     EDYERQVKIAFADDNTTADIFLFMSKIITETLKTNFYQIIKRSYAFKFTGNILDPVVF
                     QKKVESVFFVIGFYAIGTMMRAEEIARGGVRLIRVTKNNYETELDYMPLLNYELGPVA
                     QRLKHKDIAESGAKSVIVPYPEYAVEGLNSVLDFASGILDLVIVSKEIVDYHGKPEMV
                     FFGPDEGTGPMMDVVAYFAKIRKYKYWRTITTGKSTGIPHDAYGQTQDKKVFGLFPKD
                     TQTELQI"
     gene            2039..4717
                     /locus_tag="PLW07_02800"
     CDS             2039..4717
                     /locus_tag="PLW07_02800"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOL49483.1"
                     /translation="MHKFSISWIGIFLYLLAVVTQPLAAAHIDSTTRNHSISAETVLI
                     AENGTAKLPIIISEKASKDTKARADELAMYLERITGAKFEVKTGAGPGIILGTIEQFP
                     TPSASDGLKIYDVYDGRDAYAIRTEGGSIKLLGATDLGVSHAMSRFLTLLGCRWFFPG
                     PVWEVIPKISKLSFNINETDRPEVLGMHFGYDLGQQYEKTDPDAGNATKSWNRKNLLA
                     TGFKSAAGHCAHTIVNYFRKEFEQHPEYRALIEDKNGKRFRTGTQYSPTDPRWQWGQL
                     CVSNPEVVKLACQYADEFFDKHPDADMIGMGPDDGGGWCLCPECAKLGNCGNQAFYLA
                     NQVAKHIQKTHPGKLVGLLAYNWHCDPPDFPLEPNVYVELTTTLLINTKLGFDRLVEV
                     WPKKVKYCGLYDYWAVYDWTRDNMPAGRTGNLKYVAEKIPFYIKHGLTCMCAESGNSW
                     GGQGLGYYLAIHLMWNTSTDVEALKNDFYEKAFGPAAKEMKAYYQHTDLGNNPLVGPT
                     FYRICCDYLESAEKAAKGHPDVLARIEQLKQYNVYVYLLNKERDTTLPPGERKKWAFE
                     TLRWNYRMRNTYMTFWTFFAGMTSAQWAKEFKEDSWDWYKNYWVLKKPEQIPYRDSNP
                     ITPEETARWFQQMKADYGEVPKITEISFSKKLVAPGWVSDTPNPQKNDRVIFHGPFTM
                     ALASMKGEPLRFSMNHGTMYPNSPAGDYVLTDMSGKEITKGTVPICPKNPTEIELKVP
                     GAGVYYFKFDDHGGGTAFMPSSGLRAAFVPEGPRGYTLLYRVWFYVPKGTTEIQFAPS
                     CSGIRFGIQTPDGKWVECKNRVNLPARSDGLNFKADGYYYVIPVPKDMDGKMWAMSMA
                     QCGFHMRFFNIPTILSLSSDGPIIPEEVAKKDGLLK"
     gene            4729..7101
                     /locus_tag="PLW07_02805"
     CDS             4729..7101
                     /locus_tag="PLW07_02805"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOL49484.1"
                     /translation="MKPVYLIVSIFVGISLLKAAPITIVENGKPQAVIVVASDEPKAD
                     KAATEIQKYIEKMSGAKLPIVKEGEPVNTDVSIFVGHTKAAAKNGVKIPSGFKEVVGD
                     PKVFEEEGFVIKTKGKNIFIGGNSDGPYQGTIYAAYEFLERLGCRFYFPGEWGEVIPE
                     KKTITFPEIDLQSKPDFAIREIGLGGWIPSSKQEWAIYADWQNKVKMTNTFFYPPVGD
                     GFLAYLIPPNEFYPKEPELFAMNQNGSREPMKWYNGRYYENVTMLALGNPRTLEVAVQ
                     NLKDAYAGKRPRLGNMSPSGNGFGISPPDGAVFDYDPKYKDINQNFNYPTYIHHPMTS
                     EEFFTFAAKLAKEFPDKWVSTMAYAGREMPPQGVKIPQNIAVMYAPISSCVLHPGNDS
                     SCWRRSETISIMRQWCKLTPHVWLYDYNPGFLLGCFVPERDVANFAVNVKLYKDMKLK
                     GFQTEGRKAFMATWISWYIRAKLMWDAHADVEALKKDFYTTFFGPEAGLFVQQWWDEC
                     EKALGSATIHCHEDWLVNHIYTVPFTKKIHQYVEKAAKCAMTPEQKEHFNAFALIADH
                     LEAYAAKEEAEKNLDYREAARQAQRMEDDKKKLYSIYPFFIGPKYHPEFTNGQEIKYR
                     QLYAMTNGETGILIAPVPLEAKFKRDPFNEGVVGQWYLPEFDDTQWETKNTFFTWDAQ
                     DKPEDAKGHDYDGYGWYRFTLKVPSDAISKQLKLHLGGVINEGWVWINGNYVGHRQWQ
                     LWWAGREALEMDVDVTGKVKPGDNIVAVRVWNNAEIGGLFRRGFLWSPAK"
BASE COUNT         2162 a         1448 c         1594 g         2009 t
ORIGIN      
        1 caatttgaag ttctgtttga gtatccttcg ggaaaagtcc aaaaaccttt ttatcctgtg
       61 tctgaccata tgcatcgtgt ggaatgccag ttgattttcc tgttgttatt gttctccagt
      121 atttatactt ccgtattttt gcaaaatatg caacaacatc catcattgga ccggtcccct
      181 catcaggacc aaagaaaacc atttctggct ttccgtggta atccacgatt tctttagaaa
      241 cgataacaag gtcaagtatg ccactggcaa aatcaagcac agaattcaac ccttccacag
      301 catattctgg atatggaaca atcactgact ttgctccgct ttcagcaatg tctttgtgtt
      361 ttaatctctg cgcgactggt cccagctcat agttcaatag tggcatgtaa tccagctctg
      421 tttcgtaatt gttttttgtt accctgatta atctaacgcc accgcgagcg atttcttcag
      481 ccctcatcat tgtgccgatg gcgtaaaatc ctatcacgaa aaacacactt tccacctttt
      541 tctgaaagac cacagggtca aggatattgc cagtaaattt aaaagcatac gaacgtttga
      601 taatctgata gaaatttgtt ttcagtgttt cagtgataat cttgctcata aacagaaata
      661 tatctgcagt ggtgttgtcg tcagcaaaag caattttcac ctgtctttca tagtcctcaa
      721 tctggtgcaa aattttctca aaattctttt caggttgtgc tggattaaac cttttgtcaa
      781 agatggcaaa aatccttttt acaaaatcag gattctgttt gattgccgat gtaatgactg
      841 acctgctgta ttcagccctg tttgactcaa aaacccttct tgcaaacgca tctttcattt
      901 ctttatttga aaaagattcc atgatatttc tgtcaacagg gtctgtcttg tgaataaagg
      961 tgtgaacaaa aacagaagca acaaccgcaa aaagatactc aaaaaaactt aattgcccgc
     1021 cagtgtaaag ttcatcaagt ccagatggtt ggattgccag taagagatgc aacctgttta
     1081 ttgttgtctg catcttttct gaagaaatac caccttcaac ataaagagaa cagatggatt
     1141 caatccttcc tgtttttcct ctatatgttt cccagtatgc gcggttcaac gtaataccga
     1201 tttcttcaag catttttcta acagtgggca gtacagaact tgagaagtcc tctttgaaca
     1261 ttatgcggat ttcacctgtg ttttttgatt ttgaaacaaa gatgatggga acaaaacttt
     1321 tctcaacctt tttcaaaaat tcttcatatc ttttttttgt cacttctggc acaggcaatc
     1381 cagaaacgat ttcattgaac gcaaaagggg attgtcctgg ttttatatct ttcaattctt
     1441 tgcatttttc gggtctgatg atgtatgtgt agtaattgtg cttcttgcca taataatact
     1501 caatcctgtt tcctgacatt actggattta atattttttc catcctgtcg cggttttctg
     1561 ctgttgcaat tcttacctgc actccgcctt ccactgtaaa tggaacttct gagacctcgc
     1621 tccgcaatac atagaaattg ccttcttttt ctaaattgct tgcaatggtt tttaacactg
     1681 tttttagaga gtcctttgaa agtcccttga aaaaatattc tggaagtccg aggtcactga
     1741 gcaaaatacc cgcagcagca ttgatgcagg aagcagttaa tagtccttct tctgcaagtt
     1801 ctaccacaca ctcgtaaagc aaacttgcgt caagcccgca ctttttcgcc ttttcaataa
     1861 ttgaactgat ttcagtttga tttgtgtttt tcatagtact tgtttatctt actctgtttt
     1921 aaaagaactt tcaagaaaat ctttcatttc ttttcttaaa taaaaaaagt gtctttgaaa
     1981 ataccagaaa aatggtaaga tatgattgaa aatacgcgaa agtagcaaga caatgtcaat
     2041 gcataaattt agcatttctt ggataggtat ttttttatat ttgcttgccg tggttacaca
     2101 gccacttgcg gctgcacaca ttgattccac aacccgcaat cattctatca gcgcagaaac
     2161 tgtcttgatt gcagaaaacg gcactgcgaa attacccatc attatttctg agaaagccag
     2221 taaagataca aaggcgcgcg ctgatgaact tgctatgtac cttgaaagga ttacaggcgc
     2281 gaaatttgag gtgaaaaccg gggcaggtcc aggaattatt ctgggaacaa ttgaacagtt
     2341 tcccacgcct tcagcatctg atgggctaaa aatctatgat gtctatgatg gccgcgacgc
     2401 ctatgcaatt cggacagaag gtggcagtat caaactgctc ggcgcaactg atttgggtgt
     2461 ttcccatgca atgagtaggt ttttaactct gctcggttgt cgctggttct tccctggtcc
     2521 agtgtgggaa gtaattccga agatttcaaa attgagtttc aacatcaatg aaactgacag
     2581 gccagaggtg ctggggatgc atttcggata tgacctcgga cagcagtacg aaaagacaga
     2641 ccctgatgca ggcaacgcca caaagtcctg gaacagaaag aatcttctgg caacaggatt
     2701 taaaagtgct gctggccatt gcgcacatac aattgtcaat tatttccgca aggagtttga
     2761 acagcatcca gaatatcggg cactgataga agataagaat gggaaacgtt tcagaacagg
     2821 gacacaatat tcacccacag acccaagatg gcagtggggg caactgtgtg tttcaaatcc
     2881 tgaagttgtg aaattggcat gccagtatgc agatgagttt tttgataaac accctgatgc
     2941 agatatgata ggaatgggcc ctgatgatgg tggcggctgg tgtttatgtc ctgaatgcgc
     3001 caaactcgga aattgcggca atcaagcatt ttatcttgcc aaccaggtcg caaaacacat
     3061 tcagaaaacc catcctggta aacttgtggg acttcttgcc tataactggc actgtgaccc
     3121 accagatttt ccgcttgagc ccaatgtata tgttgaactg accactactt tacttatcaa
     3181 tacgaaactc gggtttgacc gtcttgtgga agtatggccg aaaaaggtca aatactgcgg
     3241 actttacgat tactgggctg tctatgactg gacgcgggac aatatgccag caggcagaac
     3301 cggaaatctg aaatatgtgg cagagaagat tccattttac atcaagcatg gtcttacgtg
     3361 tatgtgtgca gaaagcggaa acagctgggg tggtcagggg cttggctatt accttgccat
     3421 tcacctaatg tggaacacat ccacagatgt tgaagcactg aaaaacgatt tttatgaaaa
     3481 agcatttggt cctgcggcaa aggagatgaa ggcatattac cagcacactg acctgggcaa
     3541 caacccgctt gttggtccta cattttaccg catttgttgt gattaccttg aatctgctga
     3601 gaaagccgca aaaggtcatc ctgatgtgct ggcacgcatt gaacaactga agcagtataa
     3661 tgtttatgtc tacctgctga acaaggagcg agacacgaca cttccgcctg gagaacgcaa
     3721 gaaatgggct tttgaaacac ttagatggaa ctatcgtatg cgcaacacat acatgacctt
     3781 ctggactttt tttgccggca tgacatctgc tcaatgggca aaggaattca aggaggactc
     3841 ctgggactgg tataaaaact actgggtatt gaaaaaacca gaacagattc catatcgtga
     3901 ttccaatcca attacgcctg aagaaactgc ccgctggttt cagcagatga aagcagatta
     3961 cggtgaagtt ccaaaaatta ctgaaataag tttcagcaag aaactggttg ctcctggctg
     4021 ggtttcagat actccaaatc ctcagaagaa cgatagggtg atttttcacg gtccattcac
     4081 gatggctctg gcaagtatga aaggagaacc gctgcgtttt tcaatgaacc atggaacaat
     4141 gtatccaaac agccctgctg gcgattatgt tctgacagat atgtcaggca aagaaattac
     4201 aaaaggcaca gtaccgatat gtccgaaaaa tccgacagag attgaactga aagtgcctgg
     4261 tgcaggtgtc tattatttca aattcgatga ccatggtggt gggactgcct ttatgccatc
     4321 ttctggtctg cgcgcggctt ttgtaccaga aggaccaaga ggttatactt tgctgtaccg
     4381 cgtatggttc tatgttccaa aaggcaccac agagattcag tttgcgcctt catgcagtgg
     4441 aataagattt gggatacaga ctcctgacgg aaaatgggtt gaatgcaaga atagggtaaa
     4501 ccttcctgcc agaagcgatg gactcaactt caaggcagat ggctactatt atgtgatacc
     4561 agtgccaaaa gatatggatg gcaaaatgtg ggcaatgtct atggctcagt gtggtttcca
     4621 tatgcgcttt ttcaatatcc cgacaattct gtctctgtct tcagacgggc caatcattcc
     4681 agaagaagta gcgaaaaaag atggacttct aaaatgatag gaggagcaat gaagccagtg
     4741 tatttgatag tcagtatctt tgtaggaatt tctttgttga aagccgcacc aatcactatt
     4801 gtagaaaatg gcaagccgca ggcagtaatt gtggttgctt ctgatgagcc aaaggcagac
     4861 aaggctgcta cagaaattca aaaatacatt gaaaaaatga gtggtgcgaa attgcccatt
     4921 gtaaaagaag gagaaccagt caatacagat gtttctattt ttgttggtca tacaaaggca
     4981 gcagcaaaaa atggggtgaa gattccttct ggcttcaagg aagttgtggg agacccaaaa
     5041 gtttttgagg aagaaggatt tgtgataaaa acaaaaggaa aaaatatttt cattggcggc
     5101 aattcagacg gaccgtacca gggcacaatt tatgctgcgt atgaattcct ggaacggctg
     5161 ggttgccgat tttattttcc aggagaatgg ggtgaggtca ttcctgaaaa aaagacaatc
     5221 acatttccag aaatagattt acagtcaaaa cctgattttg cgattcgcga gattggactt
     5281 ggtggatgga ttccttcaag caagcaggaa tgggctatat atgctgactg gcaaaacaag
     5341 gtcaagatga ctaatacttt tttctatcca cctgtcggag atgggttcct tgcatacctg
     5401 attcctccga atgagtttta tccaaaagaa ccagaactat ttgcgatgaa ccagaatgga
     5461 agtcgtgaac ctatgaaatg gtacaatggc agatactatg aaaatgttac aatgctcgcc
     5521 cttggaaatc cgcgcacact tgaagtcgca gttcaaaacc tcaaagacgc ctatgcaggt
     5581 aagcgaccca gattaggaaa tatgtctcca agtggcaatg gttttggtat cagtccgcct
     5641 gatggcgcgg tttttgacta tgacccaaag tacaaggata tcaaccagaa tttcaattat
     5701 cctacatata ttcaccaccc gatgaccagt gaggaatttt ttacatttgc tgcaaaactt
     5761 gcaaaagagt ttcctgacaa atgggtatca acaatggcat acgctggtcg agagatgcct
     5821 ccgcagggcg taaaaattcc acaaaacatc gcagtaatgt atgctccgat ttcctcctgc
     5881 gtacttcatc ctggaaatga ttcttcttgc tggcgacgaa gtgaaacaat cagcattatg
     5941 cggcaatggt gcaaacttac cccgcatgtg tggctatatg attataatcc aggatttttg
     6001 cttggatgtt ttgtgcctga gagagatgtt gcgaactttg cagtcaatgt caaactttac
     6061 aaggatatga aactgaaagg gtttcagaca gaaggacgca aggcattcat ggcgacatgg
     6121 attagctggt acatacgggc aaagttaatg tgggatgcac atgcagatgt agaagcactc
     6181 aagaaggact tttatacaac atttttcgga ccagaggcag gcttatttgt tcaacaatgg
     6241 tgggatgaat gtgaaaaagc attgggctct gcgacaatcc attgtcatga ggactggctg
     6301 gtcaatcata tttacactgt cccattcaca aaaaagattc atcagtatgt agaaaaggca
     6361 gcgaaatgcg caatgacgcc agagcaaaaa gaacatttca atgcatttgc acttattgct
     6421 gaccatcttg aagcatacgc agccaaagaa gaagcagaaa aaaaccttga ttatagagaa
     6481 gcagccaggc aggcccagcg tatggaagac gacaagaaaa aattatattc catttatcca
     6541 ttctttatcg gtccaaagta tcatccagaa ttcacaaatg gccaggaaat caaatacaga
     6601 caactctatg caatgaccaa tggtgaaaca ggaattttga tagcacctgt tcctcttgag
     6661 gcaaaattca agagagaccc attcaatgaa ggcgttgttg gccagtggta tcttcctgaa
     6721 tttgatgata cacaatggga aaccaaaaat acctttttca catgggatgc ccaggataag
     6781 ccagaggatg caaaaggaca cgattatgat ggctatggct ggtatcgttt tactcttaaa
     6841 gtgccatcag atgcaatcag taaacaactg aaacttcatc ttgggggtgt gatcaatgaa
     6901 ggatgggtct ggataaacgg gaattatgtg gggcacaggc agtggcagct gtggtgggca
     6961 ggccgcgagg cactggagat ggatgtagat gtaacaggca aagtaaaacc aggtgacaat
     7021 atcgttgctg ttcgggtgtg gaacaatgca gaaatcggcg gcttattccg caggggattt
     7081 ttatggtcac cagcaaaata aaacctggac ttgacaaaag ttatgagcgg gggtagaatt
     7141 gcatatggga aaaacttgaa aaggaggtga gaaaaatgaa aaagggattc acgcttattg
     7201 aactgctggt ggt
//
LOCUS       DAORYU010000054         7205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_7329_length_7205_cov_3.893566, whole genome shotgun sequence.
ACCESSION   DAORYU010000054 DAORYU010000000
VERSION     DAORYU010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7205
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7329_length_7205_cov_3.893566"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /gene="amrB"
                     /locus_tag="PLW07_02810"
     CDS             <1..693
                     /gene="amrB"
                     /locus_tag="PLW07_02810"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOL49485.1"
                     /translation="CPHAGYIYSGKTAGRVYSRIVPPDTAIIIGPNHHGYGEPYAIDD
                     NDCWETPLGNVEIDDEIRSLLLGKSRYLQTDSTAHMAEHSIEVQVPFLQFINPDIKIV
                     PVVLSSYHDANPWYEIGYTIGLVLSEYKKKVLVIASSDFTHYEPAEDAEKKDTLAIGA
                     ILRLDADEFLNIVSKHDISICGFAPIVTAITAAKTLGSKKGVLVDYSHSGQVSGDFQQ
                     VVGYAGIILDKN"
     regulatory      710..905
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1004..1714
                     /locus_tag="PLW07_02815"
     CDS             1004..1714
                     /locus_tag="PLW07_02815"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOL49486.1"
                     /translation="MKVERSKGFTLIELLVVIAIIAILAAMLLPALSKARERARAAVC
                     MGNLKQIFLAFSMYVQDNDEFFPPAYYYTFSGEVDWDFATDDWVNWKPGIIGIYLNEK
                     VFQCPSRFKLKSFDKPFTGYAYNATYIGGGFSAWDGQADSPAKLSRIQNPSQTVLVAD
                     SAIWSTWTNETVANNYLRAPGDIYYFGPNVHFRHNGRANVLFCDGHVESIGTKYNTDA
                     NDNTIGDLSTDASLYDLE"
     gene            1751..2638
                     /locus_tag="PLW07_02820"
     CDS             1751..2638
                     /locus_tag="PLW07_02820"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOL49487.1"
                     /translation="MKNKFWTLLEEKKILIADGAWGTEIAKNQQIKFTYPEILNLTHP
                     EIVKKVAASYVEAGADIILTNTFGANIFKLKKYGADDKINEINQKGVEISRKAAGDNI
                     VFASMGPTGELLEPYGNITKKEMEDCFKQQAEILIKSGADGIVIETMSDINEALCALK
                     AVKSITDRTVIVSMTFSRTLNGYRTLMGTSAGECAILLKRSGADVIGSNCGSGIEDFV
                     SIVREMNAENIPIWVKPNAGIPQLVKGKTVYPDTPEHMAGYVPELIKAGSSIIGGCCG
                     TTPLHIREIKKAVSMYLKQ"
     gene            2635..3468
                     /locus_tag="PLW07_02825"
     CDS             2635..3468
                     /locus_tag="PLW07_02825"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HOL49488.1"
                     /translation="MTKKLFPIILLTLPLMLVQLLQGASAQYPSRVISLGPYVTENLL
                     LLGVDSEIIGVTIHEKDNIKKGREIIGTLLDPNLEAILKLRPDIVIASKEGNRKETVE
                     KIEKLGIRVEVLDEVTTYSDLKKNFLTLARIFGKQTIARNIVSDIEKELKRYTSQQQK
                     SQKKIFWQLGTKPLVTAGSKTYFNELSVYSGSKNIFDDIKTKYITVNIEEVIKRNPEV
                     IIAIGMGENEYVINFWKQFKDIDAVKKNKIFRVDDYMFCSPTPASFLQSIKLLTDLLN
                     N"
     gene            3483..4445
                     /locus_tag="PLW07_02830"
     CDS             3483..4445
                     /locus_tag="PLW07_02830"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOL49489.1"
                     /translation="MVIFIFAVISFIVCLSLGTENYSFTRTIRILMDLKQYPFEKTIL
                     MQVRLPRIWCGFLTGSGLAVSGLILQAILKNPLAESYTLGISGGASLGIAFGIVSGQM
                     MAVPMFAFVGSVISIMIILATASLRKMSSGTLLLLGIMLNFIFSSFSLLVITFLQKER
                     FMEAVVWFMGNLSSYHPETLKSAPLFLLPAYFITWIYWKHLNIFSLGEEKAITLGLDP
                     EKKRYLWLILTGLITGYCVSLAGLVGFVGLVIPHTVRILVGTDHKRTIPAAILVGGSF
                     LIIADTIARTIVKPIEIPVGVISGFFGGIFFVLILLKTSKKQKW"
     gene            4439..5242
                     /locus_tag="PLW07_02835"
     CDS             4439..5242
                     /locus_tag="PLW07_02835"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOL49490.1"
                     /translation="MVILELKNVSARYGKKEILHDISFQMKKGHFLGIIGPNGSGKST
                     LLKTATKIIRQYTGTVFFNGTDIKTIPVHQFAKNISFLPSDIEIAYPYTVKELLIMAR
                     YPFTSGFRNPTSRDIEIVNAVVKQMGLTQFINRTIVEMSEGEKQRVLLAQCLVQQPQL
                     VIFDEPTAHLDIGYQFSFLDLLKQYQENSSLSILAVLHDLNLASQYCDELVMLNEGSV
                     VSAGNPHEVIQYKILEEVYRTNVLVYPHPISGKPYVFGIPVSWKNLTKK"
     gene            5296..6063
                     /locus_tag="PLW07_02840"
     CDS             5296..6063
                     /locus_tag="PLW07_02840"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOL49491.1"
                     /translation="MKIAIQLYTIREYFGSQEELVKAVKKLADIGYKYVETAGTANLP
                     PKQFKQIMDDAGMTICSSHVGVDAILKEPEIVVEQQLILNAHCCNASWPGAETTNIDG
                     IKKTAEKIAKAAEVLKKNGLILGYHNHGMEFSKCSGKTWLEIIMETAKPEILTAQIDT
                     YWVQYGGGDPAYWIDRYSQRLNSIHFKDMGMSTDNKQSMVPVGTGNLNWTRILAAAKN
                     SSAKYAIMEMDFSPLVPLWDSIEMSFKNMKEWGLETD"
     gene            complement(6067..7065)
                     /locus_tag="PLW07_02845"
     CDS             complement(6067..7065)
                     /locus_tag="PLW07_02845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOL49492.1"
                     /translation="MFQRKLKNDTINSVLIVGLSSIGDNLLITPAIKLMRDALWSAEF
                     FIVIGPRAIEFAKGNPIFSDFYVWDKKTGIMTLIKMLRNQKYDLVVDFRNSFIPFLLR
                     SKYRLTFFIKELFSDKFTLHEAERTLRFFEPYFGKCDKVELYFPLTDSEIKQHFEFFK
                     NLGLMDTGKKIVVMNPGAAFEKKRWDKRNFIEVGKHIVRNYHATVAIVGNNKESDLAG
                     AIKKGINDLKVFNLAGKTTFRQLAYLLSCSELLITNDTGTMHLASAMKCPVIAIFGPG
                     NPQRYGPIGNRNIVLHTQRDCFPCVVESKCKREFICMNDVTIEDVCGAVLQMLKPF"
BASE COUNT         2401 a         1328 c         1408 g         2068 t
ORIGIN      
        1 tgtcctcatg caggatacat atattcaggt aaaacagcag gcagggttta tagtagaatt
       61 gtgccacctg atacagcaat catcatagga ccgaatcatc atggatacgg agaaccttac
      121 gcaattgatg acaatgactg ctgggaaacc ccgcttggta atgtagaaat agacgatgaa
      181 ataagaagtt tgctccttgg aaaaagcaga tatctgcaaa cagattcaac tgctcacatg
      241 gctgagcatt caatagaagt tcaggttccc tttttacaat tcattaatcc tgatataaaa
      301 attgttccag tggtactttc cagttatcat gacgcaaacc catggtatga aattggatac
      361 acaattggtc ttgtactcag tgaatacaag aaaaaggtgt tggtgattgc cagttctgat
      421 tttacccatt atgaaccagc agaagatgca gaaaaaaaag atacacttgc cattggtgcg
      481 attttacgac ttgatgcaga tgaatttttg aacatagtgt ctaaacatga tattagtatc
      541 tgtggattcg cgcccatagt aactgcaatt acagcagcaa aaactctggg ttcaaaaaaa
      601 ggcgtactcg ttgattactc acatagcggt caggtttcag gagatttcca gcaggttgta
      661 ggatatgcag gtattattct tgacaaaaat taatatagca ttataatttt atccagtaaa
      721 ttttgcaggc gcccgaagtg ggcttaatag ggaagtctgt gaaaatcaga cacgctcccg
      781 gcgctgtaac ctggctcatc aaaaggtgag caagtttgag tctgaaatac cactgtcggt
      841 aacgatggga aggtagatcc agatatcagg aagtcagaag acctgcctgc gaaacggtgt
      901 aacgtattcc tgcggagttc aggaatcgtg aaagggtgaa gaaatcaggg tgcagcccgc
      961 cgtttggcgg gctttatttt tgcctgcata acagggagac gtcatgaagg tagaaagaag
     1021 taaaggattt acgttaatag aattgcttgt ggtaattgcg attattgcaa tactggcagc
     1081 aatgttgttg ccagcattgt caaaagcacg ggaaagagca agggctgctg tctgtatggg
     1141 aaatttaaaa caaatatttc ttgcattctc catgtacgtt caggataatg atgagttttt
     1201 ccctcctgca tattactata ctttttctgg cgaagttgac tgggattttg ctactgatga
     1261 ttgggtcaac tggaaaccag gtattattgg aatttatctt aatgaaaaag tgttccagtg
     1321 tccttcaaga tttaaactca aatcctttga taaacctttc accggctacg cctataatgc
     1381 cacatacata ggcggtggtt tttctgcctg ggatgggcag gcagattctc cagcaaaact
     1441 cagcagaatt caaaatccct cccaaactgt gcttgtagca gatagcgcaa tatggtcaac
     1501 gtggacgaat gaaacagttg caaataacta tctgagggca cctggagata tttattattt
     1561 tggtccaaat gtgcatttca gacataatgg aagagcaaat gtattgtttt gcgacggcca
     1621 tgttgagtcc attggcacaa aatacaatac agacgcaaat gataatacaa ttggagacct
     1681 ttcgacagat gcatcattat atgaccttga ataattttca aaaccgtttg ccactggtat
     1741 aataacaatt atgaaaaaca aattctggac attgttagaa gaaaaaaaaa ttttgattgc
     1801 agatggcgca tggggaacag aaatagcaaa aaaccagcag ataaaattta catatcctga
     1861 aatattgaat ctgacgcatc cggaaatagt gaaaaaagtg gcagcatcct atgttgaagc
     1921 aggagccgat attatactca ccaacacatt tggagcaaac atttttaaat taaaaaaata
     1981 cggcgcagac gacaaaatca acgaaattaa ccagaaaggt gttgaaatat cacgcaaagc
     2041 cgcaggcgat aacattgttt ttgcctcaat gggcccaaca ggtgaacttc tggaacccta
     2101 tggaaatatc actaaaaaag aaatggaaga ttgctttaag caacaggcag aaatactcat
     2161 caaatctgga gcagatggca tagtgataga aacaatgagt gatatcaacg aagccctgtg
     2221 cgccttgaaa gccgtaaaaa gtattacaga tagaaccgta attgtcagta tgacattttc
     2281 cagaaccctt aacgggtata gaacattgat gggaacaagt gctggtgaat gcgctatatt
     2341 gcttaagagg tctggagcag atgtcattgg ttcaaattgc ggttcaggta ttgaagattt
     2401 tgtatcaatt gttagagaaa tgaacgcaga aaacatccca atatgggtga aaccaaatgc
     2461 aggaattccg cagctagtaa aaggaaaaac tgtatatcct gacactcctg aacacatggc
     2521 aggttatgtt cctgaattga taaaagcagg ttcttcaatt attggcgggt gttgcggtac
     2581 tacgccacta cacattcgcg aaataaaaaa agccgtgtca atgtatctaa aacaatgaca
     2641 aaaaaattat ttcctattat tttactgact ttgcccttaa tgttagtgca gttgctacag
     2701 ggagcaagcg cacaatatcc ttcacgcgtc atttctctcg gtccctatgt tacagagaat
     2761 cttcttctcc ttggtgttga cagtgaaatt attggtgtca ccatacatga aaaagataac
     2821 ataaaaaaag gcagagaaat tattggtact ctgcttgatc caaatcttga agcaatcctg
     2881 aaattaagac cagacattgt aatagcaagc aaagagggta atcgtaaaga aactgttgaa
     2941 aaaattgaaa aacttggtat cagagtagaa gtccttgatg aggttactac atattcagac
     3001 cttaaaaaaa attttttgac cctcgccagg atatttggta agcaaactat tgccagaaat
     3061 atcgtttctg atatagaaaa ggaacttaaa cgttatacca gtcaacaaca aaaatcacag
     3121 aaaaaaattt tctggcaact tggaacaaaa cctttagtaa ccgcaggaag taaaacatac
     3181 ttcaatgaac tatcagttta ttcaggatca aaaaatattt ttgacgatat aaaaaccaaa
     3241 tatatcacag tcaatataga agaagtgatc aaaagaaatc cagaagtaat tatagcaatt
     3301 ggaatgggtg aaaacgaata tgtcattaat ttttggaaac aatttaagga tattgatgct
     3361 gtcaaaaaaa ataaaatttt tcgcgtcgat gactatatgt tttgcagtcc aacaccagca
     3421 agttttttgc aatcaataaa attgctaaca gatcttttaa acaattgaaa aagatatctt
     3481 ttttggtaat ttttattttt gccgtaatat ctttcattgt ctgcctctct ctgggcacag
     3541 aaaattattc attcaccaga accatcagaa tactgatgga ccttaaacaa tatccatttg
     3601 aaaaaacgat actaatgcag gtcaggttgc caagaatatg gtgcggattt ctcactggtt
     3661 ctggtctggc agtatccggt cttattttac aggcgatttt aaaaaatcca cttgcagaaa
     3721 gttatacact gggcatatcc ggaggcgcaa gtttaggaat cgcgttcgga atagtatcag
     3781 gacagatgat ggctgtacca atgtttgctt ttgtcgggag cgttatttct attatgatta
     3841 ttcttgccac tgcgtcttta agaaagatgt cttcagggac actactgttg cttggtatta
     3901 tgctcaattt catcttttct tcattctcac tcctggtaat cacatttcta caaaaagaaa
     3961 ggtttatgga agcagttgta tggtttatgg gaaacctgag cagttatcat ccagaaacat
     4021 tgaaaagcgc tcctctgttt cttttaccag catattttat tacatggata tactggaaac
     4081 atttgaacat attttctctt ggtgaagaaa aagcgatcac acttggtctg gaccctgaga
     4141 aaaaaagata tctgtggctt atccttacag gacttattac tggatattgt gtatctcttg
     4201 caggccttgt gggatttgtg ggactggtaa taccacacac agtaagaatt ctggttggta
     4261 cagaccataa aagaactatc cctgcagcaa tactggttgg cggaagtttt cttatcattg
     4321 cggacaccat tgcacgcaca attgtcaaac ccattgaaat tcctgttggt gtaatcagtg
     4381 gattttttgg aggaatattt tttgttttga tactcttaaa aacttcaaaa aaacaaaaat
     4441 ggtaattctt gaacttaaaa atgtcagtgc acggtatggt aaaaaagaaa tccttcatga
     4501 tataagtttt caaatgaaga aaggtcattt tcttggaatt attggtccaa atggaagtgg
     4561 caaatcaacg cttctgaaga ccgcgacaaa aatcataagg cagtacacag gaaccgtctt
     4621 ttttaacgga acagacataa aaaccatacc agtccatcag tttgctaaaa atatttcgtt
     4681 cttaccatca gacattgaaa ttgcttatcc atacacggtt aaagaattac tcatcatggc
     4741 aagatatcct ttcacatcgg gttttcgtaa tcccacatca agagatatag aaatagtaaa
     4801 tgcagtagtc aaacagatgg gactgactca attcataaac agaacgattg ttgaaatgtc
     4861 agaaggagag aaacaaagag tacttcttgc gcagtgtctt gttcaacaac cacaactggt
     4921 tatatttgat gaaccgactg ctcaccttga tattggttat caattttcat ttcttgacct
     4981 cttgaaacaa taccaggaaa attcatctct ttctattctt gctgttttac atgatttaaa
     5041 tctggcttct cagtattgcg atgaactcgt aatgctcaat gaaggttcag ttgtgagtgc
     5101 agggaatcca catgaagtga tccaatacaa aatcctggaa gaagtctatc gcacaaatgt
     5161 actggtatat cctcacccca tttcaggaaa accctatgtt tttggaattc cagtatcatg
     5221 gaaaaacttg acaaaaaaat aatcatcgta tttaatatgc cagtttaaca ataattattt
     5281 caacaggaga aaaatatgaa aatcgcaatc cagctttaca caataagaga atattttggt
     5341 tctcaggaag agcttgtgaa ggcagtaaaa aaattagcag atatcgggta caaatatgta
     5401 gaaaccgcag gtaccgcaaa tctaccacca aaacagttca agcaaatcat ggacgacgct
     5461 ggcatgacca tttgctcaag ccacgttggg gttgatgcaa ttttgaaaga gccagaaata
     5521 gtagttgaac agcaattaat tctcaatgca cactgttgca atgccagctg gccaggggct
     5581 gaaacaacga atatagatgg tatcaagaaa acagcagaga aaatcgcaaa agcggcagaa
     5641 gttttgaaaa aaaatggatt gatacttgga tatcataatc atgggatgga attttcgaaa
     5701 tgcagtggta aaacgtggct tgaaataatt atggaaactg caaagccaga aatacttact
     5761 gcacagattg atacgtactg ggtgcaatac ggaggagggg acccggctta ctggattgat
     5821 agatattctc aacgattgaa ctctatccat tttaaggata tgggaatgtc cacagataac
     5881 aaacaatcca tggttcctgt tggcactggt aatctcaact ggacaagaat tcttgctgct
     5941 gcaaaaaatt caagtgcaaa atacgcaatt atggaaatgg atttttcacc ccttgtacct
     6001 ctgtgggatt ccatagagat gagtttcaaa aacatgaaag aatggggtct tgaaactgac
     6061 tgaatgttag aacggtttca acatttgcaa aacggcacca caaacatctt ctattgtgac
     6121 atcattcatg cagatgaatt cccttttgca ttttgattcc acaacacagg gaaaacaatc
     6181 tctttgagta tgaagaacaa tatttctgtt acctattggg ccatatctct gcggattacc
     6241 aggaccgaaa attgcaatca cagggcattt cattgcagaa gccaggtgca tcgttccagt
     6301 atcgttggta attaacagtt ctgaacagga aagaagatac gccagttgtc ggaatgtcgt
     6361 ttttcctgca agattaaaaa cttttaaatc attgattcct tttttgatag ccccggcaag
     6421 atcagattct ttattattcc caacaatagc gacggtagca tgataatttc gaacaatatg
     6481 tttccctacc tctataaaat ttctcttatc ccatcttttc ttttcaaacg ctgcgcccgg
     6541 attcattaca acaatttttt tgccagtatc catcaaccca agatttttaa aaaactcaaa
     6601 atgctgcttg atttcactgt cagtcaatgg aaagtacagt tccaccttat cacactttcc
     6661 aaaatatggt tcaaaaaatc tcaaagtcct ttcagcctcg tgtaatgtaa atttgtctga
     6721 gaataattct tttataaaaa atgtcagtcg gtatttactt cttaacaaaa acggaataaa
     6781 agaatttctg aaatcaacca caagatcata tttctgattt ctcagcattt tgatgagcgt
     6841 cataataccg gttttcttat cccagacata aaaatcggaa aaaattggat taccctttgc
     6901 aaactcaatg gccctcggtc caataactat gaagaattca gcactccaaa gcgcgtcacg
     6961 catcaactta attgcgggtg taattaaaag attgtctcca atagaactca ggcccacaat
     7021 taacacactg ttgattgtat cattcttcaa ttttctctga aacattggat atttcctctc
     7081 ttcaggtaaa ctatgataac acaatttttg acattttgtt tctttttcag taaaattatt
     7141 ccctgcacat atgggccgct agctcacccg gcagagcacc gcccttttaa ggcgggggca
     7201 gcagg
//
LOCUS       DAORYU010000055         7133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_7429_length_7133_cov_4.601582, whole genome shotgun sequence.
ACCESSION   DAORYU010000055 DAORYU010000000
VERSION     DAORYU010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7133
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7429_length_7133_cov_4.601582"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..887)
                     /locus_tag="PLW07_02850"
     CDS             complement(156..887)
                     /locus_tag="PLW07_02850"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOL49493.1"
                     /translation="MDGSSVDGRLENIQIVAHRGFSGIYPENTLIAFKKAIELGVYFL
                     EIDIRQTSDGEPVVMHDEKIDRTTDGTGYVRELTFSQLRKFDAGKWKGFSGEKIPHLE
                     EVLELANDATGILIEIKECDVEKLGKFLNKRKNRKFYVGSFHVEYVKKIREIIPEIPT
                     SFICGHIPTNLPELVSYGIRKLDIQFGVLNADIVKTLVSSGFLVNSWTPDSKEDLLLN
                     INMGVQFITTNRPDILKEILYIRKK"
     gene            complement(857..2272)
                     /locus_tag="PLW07_02855"
     CDS             complement(857..2272)
                     /locus_tag="PLW07_02855"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HOL49494.1"
                     /translation="MTGIYAIAPSVVATGERFNLRFKILTTPYDVGWGCYSLPPGLRS
                     PFNFSPRGIRYLDNVYDGDIGEIEIEIENRKITFSDYHGVFFNDKRKFGTVNDLWFEK
                     PGIYSIKVKYPEKNIEVMTNPVLVMDDPQFRIFWGDPHSQTFFSDGLRCPEELYMFAR
                     DEAFLDFFSVSDHSEWITDRQWEYFCAVTNDFNQNGRFVTLVAQEWTNHNFGHRNIYF
                     PGNAGPILRSGKDSIEKVYEVARQYNALVIPHHSANMSMGVNWHLAHDRDVEKLVEIY
                     SVWGNSEMSAQQGNTRPIRVLGGEKSSQHVVDALNLGYKFGFVGGGDIHDGRPGDELH
                     TDQNKPDIYKLLYRQGITAIFAKKLTRTEIFNALFHRQCYATSNIRMIVQFSINDLVS
                     GNVLRNPGKLEFSIKGASEVPIQTIYVISNGKTYRTFEIGKRECNIEFQDQYCGEKYF
                     YVRAQRIDGEMAWMGPVWMED"
     gene            complement(2274..2972)
                     /locus_tag="PLW07_02860"
     CDS             complement(2274..2972)
                     /locus_tag="PLW07_02860"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HOL49495.1"
                     /translation="MEILDRFKDEVNCGELLRKLRINEPADEEEFKRIFERSKSIARP
                     KAIYMEGYVESRNDDGVVINGVKFESATLSKNLEKSGRVFPYIATCGAELDSVKFPDD
                     FLKEYWWDAIKAAYLNIARKNLMEYIKKRFLLDKTATMVPGGSEQGLWPIQQQKQLFS
                     LFGDVEKLIGVRLTESCLMIPNKSVSGILFSTEIEYSGCKVCRRKNCPGRTAEFDQEM
                     WEKLGIEAISNLRK"
     gene            3090..3458
                     /locus_tag="PLW07_02865"
     CDS             3090..3458
                     /locus_tag="PLW07_02865"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOL49496.1"
                     /translation="MTKLQDFLHAISDEKRIRILKMLEKRPVCICQITAVLGIRQPTV
                     SKHMSKLKKANIVIEQRTGQFILYMLNKSNPFINIWWVTSNLIDSNIIMKDEKCLKEF
                     TRKVPLKIDSQSSINQENQW"
     gene            3452..4642
                     /locus_tag="PLW07_02870"
     CDS             3452..4642
                     /locus_tag="PLW07_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOL49497.1"
                     /translation="MVGRIKLKEWQKFFLMGFIFILLYFLPVEILKFRNPFYEALALV
                     KWYAREHVLLCLVPAFFIAGGISVFISQASILKYFGPKANKILAYCVASVSGTILAVC
                     SCTVLPLFSGIYKRGAGLGPAIAFLYSGPAINVLAIIMTARILGWQLGLARAICAIIM
                     SVVVGLLMHLFFIKEEIEKHNNSNSFVSEENDKHPLWQIVLQFAFMIAFLVFANWAEP
                     KAGVAGLWANVFQIKWLLSAFSLLGLFFVLKFWYDGDEIQNWTGATWVFAKQITPLLL
                     IGVFIAGFLLGRPGYEGIIPSKFVSGLVGGNSLSANFFASIVGAFMYFATLTEVPILQ
                     GLIGSGMGKGPALALLLAGPALSLPSMLVLKNVMGTKKTVVYVFIVVIISTFCGIVFG
                     NIVK"
     gene            4660..4890
                     /locus_tag="PLW07_02875"
     CDS             4660..4890
                     /locus_tag="PLW07_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOL49498.1"
                     /translation="MKKIQILGIGCPKCKKLTENAEMAAKELGIEYEIEKITDINEIM
                     NFGVMMTPALAVDGEIKVSGRVPSVEEIKKLL"
     gene            4909..5361
                     /locus_tag="PLW07_02880"
     CDS             4909..5361
                     /locus_tag="PLW07_02880"
                     /inference="COORDINATES: protein motif:HMM:NF020434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HOL49499.1"
                     /translation="MAENCCCKGPARLLFPCAGGSDVGELTDRVARKLTKDGWGKIYC
                     LAGVAAHIENFLENTKAAEEVVAIDGCPASCASKALAHTGYQPISISLKELGFMKGKS
                     PASEENINKICELIKKQDTVQKCDSDQQGPQQINNSCCSCDTENKNKE"
     gene            5365..5889
                     /locus_tag="PLW07_02885"
     CDS             5365..5889
                     /locus_tag="PLW07_02885"
                     /inference="COORDINATES: protein motif:HMM:NF025946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOL49500.1"
                     /translation="MKNKRLNIPKIILITFICIAAVTMVIANRKTEARYHQEVAVSKQ
                     NNIQPIETSKIQPEETKNTQSKTKIKQPEKNTNEKKGGLPKLIDLGADKCIPCKMMAP
                     VLEELKNQYEGKLEVKFIDVWKNPEEGDRYRIRVIPTQIFTDEKGNELYRHEGFMSKQ
                     DILDKWNELGYRFE"
     gene            5895..6596
                     /locus_tag="PLW07_02890"
     CDS             5895..6596
                     /locus_tag="PLW07_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOL49501.1"
                     /translation="MYQLFETLSHAIQGSPTVAIAASFVWGILSILLSPCHLASIPLI
                     IGFISSQGKISTKTAFYLSFIFAFGILLTITLIGIITAALGKMAGNIGPAGNYIVSAI
                     FIIIGLYLLEIINIPFLSFSSKPGTKKKGMFAAFILGFVFGITLGPCTFAYMAPMLAV
                     AFKVASTNFLYAAGLLLSYAIGHCSVIVFAGTFIESVQKYLNWGEKSKGISIIRKICG
                     VLLVICAFYLIITTQ"
     gene            6593..7012
                     /locus_tag="PLW07_02895"
     CDS             6593..7012
                     /locus_tag="PLW07_02895"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOL49502.1"
                     /translation="MKTILFLCTGNSCRSQMAEGMINHYGQGKIQAFSAGLNPSTVNP
                     YAIKVMAEIGIDISKQQSKSIEQFMGQQFDFIITLCENAKQNCPVFPGKAARLHWDIK
                     DPADATGSEEEILTEFRKCRDIIKEKITNLLKGIHSA"
BASE COUNT         2290 a         1418 c         1258 g         2167 t
ORIGIN      
        1 agaattctta atctcttccc ttatggctat tgttgttctg gctttcttgt gaatccttat
       61 ttccatacgc cctattatat catagaacgt atcgttagtc aacagttaca ttcgctattg
      121 ccgaatgcta aaatctgaca taatgtcatc agaaattatt ttttcctgat ataaagaatt
      181 tccttaagga tatcgggtct gtttgttgtg ataaactgga ctcccatgtt gatattcaaa
      241 agcaggtctt ccttactatc aggggtccat gaattgacaa gaaaaccaga cgacacaagt
      301 gttttcacta tatctgcgtt caaaacgcca aactgaatgt caagttttct gataccataa
      361 gacaccagtt caggtaaatt ggttggaata tgtccacaga taaaggaagt cggtatttct
      421 ggaataattt ctcttatctt ctttacatat tcaacatgaa acgagccaac atagaatttt
      481 ctattttttc tcttattcaa aaatttaccc agtttttcta catcacattc tttaatctcg
      541 atcaaaattc ccgttgcatc atttgccagt tctaagacct cttcaagatg cggaattttt
      601 tcgccagaaa atcctttcca ctttccagca tcaaattttc gtaattgact aaaagtcagt
      661 tccctgacat aaccagttcc atcagtggtt ctatcaatct tctcatcgtg cattacaaca
      721 ggttcaccat ccgatgtctg gcgtatgtca atttctaaaa aatacactcc cagttcgatt
      781 gctttcttga acgcaatgag cgtattttcc gggtaaatac cgctgaaacc tctgtgggca
      841 acaatttgaa tgttttctaa tcttccatcc acactggacc catccacgcc atttctccat
      901 caatccgttg cgctcttaca taaaagtatt tttctccaca atattggtcc tgaaattcaa
      961 tattgcattc cctcttacca atctcaaacg tcctgtacgt tttaccattt gaaattacgt
     1021 aaattgtttg aatcggaact tcacttgctc ctttaataga aaattcaagt ttaccaggat
     1081 ttctcaaaac atttcctgac actaagtcat tgattgaaaa ttgtacaatc attctgatat
     1141 tgcttgtagc atagcactgc ctgtgaaaaa gagcattgaa aatctcagtc ctggtaagtt
     1201 tctttgcgaa aatcgctgta attccctgcc tataaagtaa tttataaata tctggtttgt
     1261 tctgatcagt atgtaattca tctcctggtc tgccatcatg aatatcacca ccaccaacaa
     1321 agccaaactt atacccgaga ttcaaagcat ccacaacatg ctgggaactt ttttcaccgc
     1381 ctagcaccct gattggtctg gtgttaccct gctgggcact catctcacta ttaccccaga
     1441 cagaataaat ttcaacaagt ttttcaacat ccctatcatg agcaagatgc cagttaactc
     1501 ccatcgacat attggcactg tgatgaggta ttacaagggc attgtattga cgcgccacct
     1561 cataaacttt ttctatactg tctttgccag accgtaaaat cggacccgca tttccaggaa
     1621 aataaatatt cctgtggcca aaattatggt ttgtccattc ctgggcaact aaagtgacaa
     1681 atcgaccgtt ttgattaaaa tcgtttgtaa cagcacaaaa atattcccat tgtctatcag
     1741 taatccattc gctgtggtca gaaacagaaa aaaagtccag aaacgcctca tcccttgcaa
     1801 acatatacaa ttcttcagga catctcagtc catcggaaaa aaaggtttga gaatgagggt
     1861 ctccccagaa gattctgaac tgtgggtcat ccatcacaag aactggattt gtcatcactt
     1921 caatattttt ttcaggatat ttcactttta tagaatagat accgggtttt tcaaaccaca
     1981 aatcattaac agtcccgaac tttcttttgt cattaaagaa aaccccatga taatcggaaa
     2041 atgtaatctt tctgttttct atctcaattt ctatttcacc gatatctcca tcatacacat
     2101 tgtctaaata cctgatgccc cttggagaaa aattaaatgg actgcgtaat ccaggaggca
     2161 gtgaataaca tccccaacca acatcataag gagtggttaa aattttaaat ctcaggttaa
     2221 acctttctcc tgttgctaca acagatggag caatagcata tattcccgtc atttcatttc
     2281 ctcaggttgg aaattgcttc tattccaagt ttctcccaca tctcctggtc aaattcagct
     2341 gttcttcccg ggcaattttt tcttctgcaa accttacatc cactatattc aatctctgtg
     2401 gaaaacaaaa ttccagaaac acttttattt ggtatcataa ggcaactttc agtcagtctc
     2461 accccgataa gtttctcaac atctccaaaa agagaaaaca actgcttctg ctgctgaatt
     2521 ggccagagcc cctgttcact accaccagga accatcgtcg cagttttatc aagtaagaac
     2581 ctttttttta tgtattccat aagatttttt ctggcaatgt tcaggtaagc tgcttttatt
     2641 gcatcccacc agtattcttt cagaaaatca tctggaaatt tgacagaatc aagttcagct
     2701 ccgcatgtcg ctatataagg aaacaccctg ccggatttct caagattctt gctcagggtt
     2761 gcactttcaa attttacgcc attgattacg acaccatcat catttcttga ttcaacataa
     2821 ccttccatgt aaattgcctt aggtcttgca atactttttg aacgttcaaa aatccttttg
     2881 aattcttctt catcagcagg ctcgtttatc ctgagttttc tcaacaattc accgcaattt
     2941 acttcatcct tgaatctatc aagtatctcc attaccccct ccaagaaaag ctttaagtta
     3001 aagtataata caacaaaaaa aaatattttc aaaaatactt gacagaaaaa ttattttttg
     3061 tattatatag ccatatggct ataaatcaaa tgacaaaact tcaggatttt ttgcatgcga
     3121 tttctgatga aaaacgaatc aggatactca agatgcttga aaaaagacct gtatgtatct
     3181 gtcagatcac tgctgtttta gggatcaggc aaccaacagt ttcaaagcat atgtcaaaat
     3241 tgaaaaaagc aaacattgtt attgaacaaa gaaccgggca atttattcta tacatgctca
     3301 ataaaagtaa tccttttatc aacatatggt gggtaacaag taatctgatt gatagcaata
     3361 tcataatgaa agacgaaaag tgtttgaaag aattcacgag aaaagttcca ttgaagattg
     3421 atagccaatc gtctataaat caggagaatc aatggtaggg agaattaaat tgaaagagtg
     3481 gcagaagttt ttcttaatgg gttttatttt catcctgttg tattttttgc cagtagagat
     3541 attaaagttc agaaatcctt tttatgaagc acttgccctt gtaaagtggt atgcaaggga
     3601 acatgttctt ttatgtctgg tccctgcttt ttttattgct ggtggcatat ctgtttttat
     3661 cagtcaggcc tccattctaa aatacttcgg accaaaagca aataaaattc tggcttactg
     3721 tgttgcatct gtttcaggga caatactggc tgtctgctcc tgcacagtac tgcccctttt
     3781 ttcaggaatc tataaaaggg gcgcaggtct tggacctgca attgctttcc tttactcagg
     3841 accagccatc aatgtgctgg caatcattat gacagcgcgt attcttggat ggcaacttgg
     3901 tcttgcaagg gctatctgcg ccatcataat gagtgttgtc gtgggactgt taatgcattt
     3961 atttttcatc aaggaagaaa tagaaaaaca caataattca aactccttcg tcagcgaaga
     4021 aaacgataaa catcctttat ggcagatagt tctccagttt gctttcatga tagcattcct
     4081 ggtatttgca aattgggcag aaccaaaagc aggtgttgct ggactgtggg caaatgtatt
     4141 tcagataaaa tggctcctga gcgctttttc tcttctgggt ttattttttg tattaaaatt
     4201 ctggtatgat ggagacgaga ttcaaaactg gacaggagca acatgggtat ttgcaaaaca
     4261 aattactcca ttgctactga tcggggtctt tattgcagga tttttgcttg gcagaccagg
     4321 ttatgaagga attattcctt caaaatttgt gtcaggactt gttggcggaa attctttgtc
     4381 agccaatttc tttgcctcga ttgttggagc attcatgtat tttgcaacct taacagaggt
     4441 tccaatttta cagggactta ttggttctgg aatgggaaaa ggtcctgccc ttgctttgct
     4501 tcttgcagga cctgcattga gtttaccaag catgctggta ctgaaaaatg tcatgggtac
     4561 taaaaaaact gttgtttatg tcttcatagt tgtcataatt tcaacatttt gcggcattgt
     4621 tttcggaaat atcgtcaaat aacaaaaaag ggggaagcaa tgaaaaaaat tcagattctg
     4681 ggcataggtt gcccgaaatg caaaaaactt accgaaaatg ctgaaatggc tgcaaaggaa
     4741 ctcggaattg aatacgaaat agaaaaaatt acagatatca atgagattat gaattttggt
     4801 gtaatgatga ctcctgccct tgcagtcgat ggagaaatca aggtttcagg tcgagtacca
     4861 tcagtagagg aaataaaaaa attgctttaa taaaataagg aggaaaaaat ggcagaaaac
     4921 tgttgttgca aaggtcctgc aagattgctt tttccatgtg ctggcggttc tgatgtcgga
     4981 gaacttactg atagggttgc aagaaaatta acaaaagatg gctggggtaa aatttattgt
     5041 ctggcaggcg tcgctgccca tatagaaaac tttcttgaga atacaaaagc agcagaagaa
     5101 gtagttgcaa ttgatggttg ccctgcttca tgcgcatcaa aagcattagc ccacactggt
     5161 tatcaaccca tcagtatctc acttaaagaa ctgggattta tgaaaggcaa atctccggca
     5221 agtgaagaaa atatcaataa gatatgtgaa ttaatcaaga aacaggatac tgtacaaaag
     5281 tgcgattctg accaacaggg ccctcaacaa ataaacaatt cgtgctgtag ttgtgataca
     5341 gaaaataaaa acaaggagta accgttgaaa aataaaagat taaacattcc aaaaattatc
     5401 ctcattacat ttatatgtat agcagcagta acaatggtta ttgctaacag aaaaacagaa
     5461 gcaaggtacc atcaagaagt tgctgttagt aaacagaata acatacaacc catagaaaca
     5521 agtaaaattc aaccagaaga aacaaaaaac acccaatcaa aaacaaaaat caaacaacca
     5581 gaaaaaaata cgaacgaaaa aaaaggtggt ctgcccaagc ttattgacct gggtgccgac
     5641 aaatgtatcc catgtaaaat gatggctccg gtcttagaag aactaaaaaa tcagtatgaa
     5701 ggaaaactcg aggttaaatt cattgacgta tggaaaaatc cagaagaagg agatagatac
     5761 agaatcaggg taataccaac acagatattc actgatgaaa aaggaaatga attgtacaga
     5821 cacgaaggtt tcatgtctaa acaggatatt ctggataaat ggaatgaact gggatacagg
     5881 ttcgaataat aaaaatgtat caattatttg aaacactttc gcatgcaatc cagggttccc
     5941 caacagttgc aattgccgct tcttttgtat ggggaattct cagtatcctt ttgagcccct
     6001 gccatcttgc aagcattcct cttatcatag gatttatcag cagtcaggga aaaatatcaa
     6061 caaaaaccgc attttattta tcattcattt ttgcattcgg gatactgcta acaattacac
     6121 ttatcggcat tatcactgca gcactgggaa aaatggctgg aaatatcgga cctgctggaa
     6181 attatattgt ctcagcaatt tttataatca ttggacttta tctgcttgaa atcatcaata
     6241 ttccttttct ttcattttct tcgaaaccag gtacgaaaaa aaagggtatg tttgctgcct
     6301 ttattcttgg ctttgtcttt ggcattacac tcggaccctg cacatttgcc tacatggcac
     6361 cgatgcttgc agtggcattc aaagttgcct caacaaattt tttatacgct gcaggattac
     6421 ttctttcata cgcaatagga cattgttcgg tcattgtgtt tgcaggaaca ttcattgaaa
     6481 gcgtccagaa atatctgaac tggggagaaa aatctaaagg aatcagtatc atcaggaaaa
     6541 tatgtggtgt gcttttggtt atttgcgctt tttatcttat cattacaaca caatgaaaac
     6601 aattttattt ctttgcactg gaaactcttg taggagccag atggcagagg gaatgataaa
     6661 tcattatggt cagggaaaaa ttcaagcatt cagcgcagga ctgaatccat caaccgtaaa
     6721 tccatatgcc atcaaagtaa tggctgaaat tggtatagac atatcaaaac aacagtcaaa
     6781 aagcattgaa caattcatgg gtcagcaatt tgactttatc ataaccttat gtgaaaacgc
     6841 aaaacaaaat tgtcctgttt ttccaggcaa agcagcaaga ttacactggg atattaagga
     6901 ccctgccgat gccactggca gcgaagaaga aattttgact gaattcagaa aatgcaggga
     6961 catcattaaa gaaaaaatta caaacctttt gaagggcatt cattccgcct gaaatttttt
     7021 aataatctgc cttcgataaa aatacaaaca ataagaacca cgcatggaaa aattaaaaaa
     7081 tgctttattc cagcgttcag cataaggcct gaaatgaacg gaccgcacac aga
//
LOCUS       DAORYU010000056         7011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_7575_length_7011_cov_4.088413, whole genome shotgun sequence.
ACCESSION   DAORYU010000056 DAORYU010000000
VERSION     DAORYU010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7011
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7575_length_7011_cov_4.088413"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1110)
                     /locus_tag="PLW07_02900"
     CDS             complement(<1..1110)
                     /locus_tag="PLW07_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49503.1"
                     /translation="MITVEQAYIRGRQSGEAASKDPMWRLMAESVMDWARKSYPASVE
                     LDYKHEILTLEKERLNALLDLSKFPECKGWMDVVRAEHKGFFDGCRDEWMTAHHFNWY
                     WFVSRRLNTRYVRKIAPKGKCTGVWFGHGKEGPMAGQNLDDLVRPFQTFNPPEKGPSG
                     KPFTKITLVGSASSAVLCDEEPENIFPVDVFEIMPQDIKKLTDFVDFLYRYREFFGPG
                     NMVFADPEMNSVAIEKSNCRMGVRWQKNGASAVTACAYLTPEMNAFKKERDRISFELR
                     GFDPVDNPDYVFWDGCERRYRRLLQLVENEASDTPSIEGLANIMLDHAVPFPDRICLA
                     GEKGHRLDWEANWTLVSSSSVLFGPARRTLWWRKEG"
     gene            complement(1119..4715)
                     /locus_tag="PLW07_02905"
     CDS             complement(1119..4715)
                     /locus_tag="PLW07_02905"
                     /inference="COORDINATES: protein motif:HMM:NF018757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HOL49504.1"
                     /translation="MDPHGIREALVIEQANQAGYYFDEIISDLPFPMRVRKSYYIRKN
                     LPLTYPLPKRIDETFQGRTGLLAYDLIVISGVDVHEFTPEEQLNLISYVEHGGALIVT
                     GGSYAFEKSDGTYHLLGSILPVEIAFPEKSNRVSNPSGIPDGFTQIQGVKLNKHHPVC
                     KGLSSEMGTITFLHPLKEKKTAQVVARAGKYPIIITWNYGNGAVAVIAARPDYDNLKG
                     IIDQTSFFEHSQYDDLMRNLIMCLVKKEKDIIIKEFSVKQKKSGLEIKAELESIKEKN
                     KKAILEIYRNNLLYQANGRTSDFSELVVQKSFEIKDKKIKTNVQIPVVLNLQPQHFFK
                     IVLTVQYGKDIDRETKNMRIDPYEPVLHRRDTVIEINQTPSIKIKNLNEEYVFMPGQT
                     LRFEINPVPARLEKLVIEIKDPHGRVVMSREQINPGSHYIFQYDIPNWRTGKYLLRAT
                     GITRSQSYYAEYEFAVIHVEPVEDLLFVAPVHVNGLTEARIREDVQDRVSKGFNCLIS
                     GKVMGKFPERFYRNLLYYRYLTQFEHKVYLWGEYEGLTCFVDHGPWYIEEGKNPNVPC
                     ILTDEFTEKTFNVVKPIIERAEKIPALLSMEILDEPHALPANICRCEVCLGSFRKKYG
                     YEMPRWSETAETGGKKRYDFFDFLGDYYEKAFTDTYNAVLKFRSRVQANHVFCLIGAG
                     QYSTHFSYTDDLKWFPYCDTFEFDCYNYMYAHFAGAEQAKFHQFHFAFARYRTLASHY
                     KKQIGFFIQLDDRDYPHDIEPIKAPCEILYTAIGQGAKTFHLMYKPTFSNVFGLRQER
                     WELFGKELKRIRNASPLLKKLNKPGSSVAMVFPYTNWVVNPQPKKLPPGYAGIGFYHK
                     EERPYNKWYPTGYTPFNSYEFIFRYFGECDVVEERLVAKGEIGDYKMLVLLATDFIHQ
                     QAAEKIEQFVKDGGVLLVDKFPSFNQNGERIDFMKNCEFSSETVFDEVELRTAIYGKG
                     RIYFLTADLDELYTDILVSEDRRRERLLVEKFQQIMFEREKIFPGCRSTNPMVEASIL
                     KNDEFTCITVVNHSQKQEKTIVIVYQSDYEIHYAVDLITFEKLPVRKNGSIIEFDCEL
                     EDLSGRFIGLYQNFADSIECEGKSRYRTGENFYLKILPISDEKPVKGEFLARLRCINP
                     EGDIDCDKIVSINSSGYIIGKTIPENEITGTWVIEFELLHSGATKKFVWDIT"
     gene            4844..6685
                     /locus_tag="PLW07_02910"
     CDS             4844..6685
                     /locus_tag="PLW07_02910"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HOL49505.1"
                     /translation="METLLFLPLPRTIKPGNHYCKLPSHAGFFISGIDEKQYCFFSEY
                     IKKIAQKYAKSRWKPDGKNPFFTLVCDRSSGIPQQGYRLRISPNSIKAESSDAAGAYY
                     ALCTLRQILIQKKNRLPALTIDDYPDFKHRGVMIDISRSKVPTMETLYSLVDLLSGWK
                     INQLQLYTEHTFAYKGHEIVWQGKSPITAEEVRKLDDYCKQRFIELVPNQNSFGHFHR
                     WLEYPEYRHLAEDPEHPYTLCPIDPGSIELLEDLYQQLLPNFSSNQFNVGCDETSIGR
                     RSIDAVKEKGEGRVYLEFLLKIYQLVKKHRRTMQFWGDIIQRHPELIPELPEDVIVLE
                     WGYEAEHPFEQRCRNIAQTKIPFYVCPGTSSWNSITGRTENCLGNLVNAAKNGIRFGA
                     TGMLITDWGDNGHWQYLPVSYIGFGFGAGVSWCLKSNLDMPVEKTIGLFAFDDRSFNT
                     GYFAYQIGNTGRATGIHTTNASSLFISLRESLVEKDEMYRSPNISLWIRTHGIEMAEK
                     HIENALGYLKRAKIENNERILILDECQNATKLLKHACKKARMIIEKYKGKGYQEKTLK
                     KMIRDAEEIIRCHKKLWLKRNRPGGLEESVELLGKFTVNQYKEILKL"
     gene            complement(6680..6937)
                     /locus_tag="PLW07_02915"
     CDS             complement(6680..6937)
                     /locus_tag="PLW07_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018194896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49506.1"
                     /translation="MAEEKEVGKVTHYYGKIGVGIVELSDGLKVGDTIHIKGHTSDFT
                     QTVESIQVEHESVSEAKPGDVVGMKVIDKVHPGDIVYKVVS"
BASE COUNT         2093 a         1492 c         1232 g         2194 t
ORIGIN      
        1 tccttctttt ctccaccaca atgtccttct tgctggacca aacaaaacag aactgctgct
       61 gacaagtgtc cagttggctt cccagtcaag ccggtgtccc ttttctcctg caagacagat
      121 tctatcagga aacggtactg catggtcaag cataatgttt gccagacctt caatagatgg
      181 tgtatctgat gcttcgtttt ccaccagttg caagagccgt ctgtatcttc tttcgcaacc
      241 atcccagaac acatagtcag gattgtcaac agggtcaaaa ccccttaatt caaaggaaat
      301 tctgtctctc tcttttttga aggcattcat ttctggagta agatacgcac acgcagtcac
      361 agcagaagca ccatttttct gccatcttac ccccatcctg caatttgact tttctattgc
      421 aactgaattc atttcagggt ctgcaaacac catgttacct ggtccaaaaa actctctgta
      481 tctgtaaaga aaatcaacaa aatccgtaag tttcttaata tcctgcggca taatttcaaa
      541 aacatccaca gggaaaatat tttctggttc ttcatcgcat aatacagcag acgacgcgct
      601 acccacaagc gtgatttttg taaatggctt gcctgaaggt cctttttctg gcggattaaa
      661 cgtttgaaat ggcctgacga ggtcatcaag attttgacca gccattggac cttccttgcc
      721 atgtccaaac caaacacctg tgcactttcc tttaggcgca atttttctca catatcttgt
      781 attcaaccgt ctgctgacaa accagtacca gttaaaatgg tgggctgtca tccattcatc
      841 cctgcatcca tcaaaaaatc ctttgtgctc tgccctcaca acatccatcc agcccttgca
      901 ttctggaaat tttgacaggt ccagtaaagc attcagtcgt tctttttcaa gtgttaaaat
      961 ctcgtgtttg taatctaatt ctacagacgc tggataactc tttcttgccc agtccatcac
     1021 gctttcagcc attaaacgcc acatggggtc ctttgatgct gcttcaccag attgtcttcc
     1081 tcttatgtac gcctgctcaa cagtgatcat aggtcctcct atgtgatatc ccagacaaat
     1141 ttttttgttg cgcctgaatg gagcaattcg aattcaatca cccaggttcc tgtaatttca
     1201 ttttcaggaa ttgttttgcc aatgatataa ccagaactat tgatgcttac tattttatca
     1261 cagtcaatat ctccttcagg atttatgcat cgcaaacgag caaggaattc accctttaca
     1321 ggtttttcgt ctgaaatcgg tagaattttg agataaaaat tttcacctgt gcgatatctt
     1381 gactttcctt cacattctat gctgtctgcg aaattttgat ataagccaat aaatcgtccc
     1441 gaaaggtctt caagttcaca atcaaactct atgatagaac catttttcct tactggcaat
     1501 ttttcaaatg taatcaggtc aactgcataa tgaatctcgt aatcggactg atacacaatt
     1561 acaatagttt tctcctgttt ttgagaatgg ttcaccactg tgatacatgt aaattcatca
     1621 tttttcaata tgcttgcttc aaccattgga tttgtactgc ggcaaccggg aaatattttt
     1681 tcacgctcaa acatgatttg ttgaaatttt tctactagca atcgctctct tcttctgtct
     1741 tctgaaacca aaatatcagt atacagttca tcaaggtctg ctgtcagaaa ataaattctg
     1801 ccttttccat aaatggcagt ccttaattcc acttcatcaa acactgtttc agaagaaaac
     1861 tcacaatttt tcataaaatc aattctttct ccattttgat tgaacgatgg aaatttgtcc
     1921 accagcagaa cacctccatc ttttacaaac tgctctattt tttctgctgc ctgctggtgt
     1981 ataaaatcag tcgcaagaag caccagcatt ttgtaatcac ctatctcacc ctttgcaaca
     2041 agtcgttctt caaccacatc acattctcca aaatatctga aaataaattc ataagaatta
     2101 aaaggagtat aaccggttgg ataccactta ttgtaaggtc tttcttcctt atgatagaaa
     2161 ccaattcctg cataacctgg tggaagtttc tttggctggg gattaacgac ccagtttgta
     2221 tatggaaaca ccattgcaac agaagaacct ggcttattca gtttttttaa tagtggcgat
     2281 gcattcctga ttcttttcaa ttcttttccg aaaagctccc atctttcctg tctaaggcca
     2341 aacacattgg aaaaggttgg cttatacatc agatgaaatg tctttgcacc ctgtccaatg
     2401 gctgtgtaca aaatttcaca tggcgcctta atcggctcaa tgtcatgggg ataatccctg
     2461 tcatcaagct ggataaaaaa tccaatctgt tttttgtaat gtgaagcaag tgttctgtat
     2521 cttgcgaaag caaaatgaaa ttgatgaaat tttgcctgtt cagcgccagc aaaatgcgcg
     2581 tacatatagt tataacaatc aaattcaaat gtatcgcaat aaggaaacca ttttaaatca
     2641 tcagtataac tgaaatgtgt tgaatattgc ccggcaccaa taaggcagaa cacatgattt
     2701 gcctggacgc ggctgcggaa tttcaacact gcgttatagg tatccgtgaa tgccttttcg
     2761 taataatcgc caagaaagtc aaaaaaatca taacgttttt tgccaccagt ttctgcggtt
     2821 tctgaccagc gtggcatttc gtatccatat ttttttctga aactaccaag acacacctcg
     2881 cacctgcata tatttgcagg aagcgcatgc ggttcatcaa gtatttccat gcttaacagt
     2941 gctggaattt tctctgcccg ttctatgatt ggttttacaa cattgaaggt tttttcagta
     3001 aattcatcag tgagaatgca cggcacattt ggatttttcc cttcttcaat gtaccaggga
     3061 ccatggtcaa caaaacatgt aagcccttcg tattcacccc acaaataaac cttgtgttca
     3121 aactgtgtca gatacctgta atataaaagg ttcctgtaga atctctctgg gaattttccc
     3181 attaccttgc ctgaaatcaa gcaattgaat cccttactta ctctatcctg tacatcttcc
     3241 ctgattcttg cttcggtcag tccattcaca tgcacaggtg ccacaaaaag taaatcttcc
     3301 acaggttcta cgtgaatgac ggcaaattcg tactctgcat aataactctg agaacgagtg
     3361 atacctgttg cccttaaaag atatttccct gttcgccagt ttggaatatc atactggaaa
     3421 atataatgac tgcctggatt aatctgttcc ctgctcataa caacgcgacc atgcgggtct
     3481 ttgatttcaa tgacaagttt ctcgagccgg gcaggaactg gattgatttc aaatctgagt
     3541 gtttgtcctg gcataaatac atactcttcg ttgagatttt taatttttat actaggggtc
     3601 tgattgattt caataactgt atctcgtcta tgcaagacag gttcataagg gtcaattctc
     3661 atattttttg tctctctatc aatatccttt ccatattgca cagtaagcac aatcttaaaa
     3721 aaatgttggg gttgaagatt cagaacaaca ggtatctgta cattggtttt tattttcttg
     3781 tcttttattt caaaactttt ttgtacaacc agttctgaaa agtcagatgt tctgccattt
     3841 gcctgataca ataaattatt tctgtaaatt tcaagtattg cctttttgtt cttttctttt
     3901 attgactcaa gttctgcttt aatttccaac cctgattttt tttgtttgac agaaaattcc
     3961 ttgatgatga tgtctttttc ttttttcaca agacacatga tgaggtttcg cattaaatca
     4021 tcgtactgac tgtgttcaaa gaaacttgtc tggtcaataa ttcccttcaa attatcgtaa
     4081 tcaggtcttg ctgcaatcac tgccactgct ccatttccat agttccacgt gataataatc
     4141 gggtattttc ctgctcgtgc cactacctgc gctgttttct tttctttcag tggatgaaga
     4201 aaggtaattg ttcccatttc actggacaat cctttgcata caggatggtg tttgttaagt
     4261 ttcacacctt gaatctgagt aaatccgtct ggaattccag atggatttga taccctgttt
     4321 gatttttcag gaaacgcaat ttcaacaggc aaaatactcc caagtaaatg atatgttcca
     4381 tcactttttt caaaagcata actgccacca gtaacaatta atgcgccgcc atgctcaaca
     4441 tatgaaatca aattcaattg ttcttcagga gtaaattcgt gaacatcaac gcctgaaatg
     4501 acaatcaagt cataggcaag cagcccggtt ctaccctgaa atgtttcatc aatccttttt
     4561 ggcaatggat aggtcaatgg caaatttttt ctgatgtaat aactttttct cactctcatt
     4621 ggaaacggaa gatcactgat aatctcatca aagtaataac ctgcctggtt tgcctgttca
     4681 atcacaagcg cttctctgat tccatgggga tccatcctga ctccttttta ctttgttaaa
     4741 atttaagcga tgataattta ttgtatagca gattgcttgt caaatatatc tgatttgaac
     4801 agaaagacaa ctatgatatc atctattgaa aagggagatg ccaatggaaa cactactttt
     4861 tttaccattg ccacgaacaa ttaaacctgg aaatcactat tgcaagctac cttcacatgc
     4921 agggtttttc atatcaggga ttgatgaaaa acaatattgt tttttctctg aatatattaa
     4981 aaaaattgca caaaagtatg caaagagtcg atggaaacca gatggaaaaa atccattttt
     5041 tactcttgtt tgcgaccgtt cgtcaggtat tccccagcag ggatacagat taagaatttc
     5101 acccaattca ataaaagcag aaagttcaga tgctgctggc gcatactatg ccttatgcac
     5161 attgagacaa attcttatcc agaaaaaaaa tcggctacct gcacttacca ttgatgatta
     5221 tcctgatttt aaacacaggg gtgtgatgat tgacatcagc agaagcaagg ttccaacaat
     5281 ggaaaccctt tacagtcttg ttgaccttct ttcaggatgg aaaattaatc aattacaact
     5341 ttatacagaa catacatttg cctataaggg acatgaaatt gtctggcagg gaaaatcacc
     5401 gatcacagca gaagaagtaa gaaaacttga tgactattgc aagcaaaggt tcattgaact
     5461 ggtaccgaac cagaatagtt tcgggcattt ccacagatgg ctcgagtacc ctgagtacag
     5521 acatcttgct gaagaccctg agcatccgta tacactgtgt ccgatagatc caggttctat
     5581 tgaattgctt gaagaccttt accagcaact acttccaaat ttctcaagca atcaattcaa
     5641 tgttggttgc gatgaaacct ccataggaag aagaagcata gatgctgtga aagaaaaggg
     5701 cgaaggcagg gtatatcttg agtttttact aaaaatttat caactggtaa aaaaacacag
     5761 aagaacaatg cagttctggg gagatattat ccagcgtcat cctgaactca tacctgaact
     5821 accagaagat gtcatcgtac ttgaatgggg gtatgaagca gaacatcctt ttgaacaaag
     5881 atgcaggaat atcgcacaga caaaaattcc tttttatgtg tgtcctggaa catcatcatg
     5941 gaattcaatt actggaagaa cagaaaactg tcttggaaac cttgtgaatg cagcaaaaaa
     6001 cggcatcagg tttggtgcaa ctggaatgct gattactgac tggggagaca acggccactg
     6061 gcagtatctg cctgtcagtt atattggttt tggttttggt gctggtgttt catggtgttt
     6121 gaaaagcaat cttgatatgc cagttgaaaa aacgattgga ctgtttgcct ttgatgacag
     6181 aagttttaat accggttatt ttgcctatca gattgggaat actggaagag ccacaggaat
     6241 tcatacaaca aatgcatcat ctttgtttat ctcactccgc gaatcccttg ttgaaaaaga
     6301 cgaaatgtac agaagtccaa atatcagttt atggataaga acacacggta ttgaaatggc
     6361 ggaaaagcac atagaaaatg cccttggata tcttaaaaga gcaaagattg aaaacaacga
     6421 aagaatcctt atccttgatg aatgtcaaaa tgccacaaaa ttgttgaagc atgcctgtaa
     6481 aaaagcacgg atgattatag aaaaatataa aggcaaaggg tatcaggaaa aaacattaaa
     6541 aaaaatgata cgggatgcag aagaaatcat tcgctgccat aaaaaactgt ggttgaaaag
     6601 aaatagaccg ggtgggcttg aagaaagcgt ggaactactt ggaaaattta cagtcaacca
     6661 gtataaagaa attttaaaat tatgaaacta ccttgtaaac gatatctcca ggatgaactt
     6721 tgtcaatcac cttcatacca acaacatctc ctggctttgc ttcagaaaca ctttcgtgtt
     6781 caacctgaat tgattccact gtctgggtga agtcagaagt atgtccctta atatggattg
     6841 tgtccccgac ttttaaacca tcagacagct ccacaattcc tacgcctatt ttgccgtaat
     6901 aatgcgtaac cttgcccact tccttttctt ctgccatgct gcctcctttg tgaatatggt
     6961 ctcacccttc atataccaag atatccctgt tttgaaatca agtcaaattt t
//
LOCUS       DAORYU010000057         6972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_7635_length_6972_cov_5.407691, whole genome shotgun sequence.
ACCESSION   DAORYU010000057 DAORYU010000000
VERSION     DAORYU010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6972
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7635_length_6972_cov_5.407691"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..1321)
                     /locus_tag="PLW07_02920"
     CDS             complement(29..1321)
                     /locus_tag="PLW07_02920"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOL49507.1"
                     /translation="MNRTDYLIIGNSVSGVNAIEGIREYDSKGEILVVSEEPFENYSR
                     PLISYFLGRKISQQQTRFRDESFYSDMKAHLLINNPAISLDCSNHAIELPAGKKIRYQ
                     KLLIATGAKPWFPPIDGLSNQLEGIFTFYSLNDAISLAQYIESVNPDNSVILGAGLIG
                     LKAAEGLLMRNIPVTIIEMADRVLPNTMDVKGSEIIQSYLARHNSEFIKNDTIIRIES
                     ENNKIKRVILKSGKQIETECLIIAVGVRPATTIVKNSPIVVNRGIVVDNNLKTNVSDV
                     FAAGDVAEAPDFFENKNALIPIWPVAAKTGKVAGSNMTGQNISYAGLYPMNSIQIFEL
                     PAISFGITNPQSKNFEILEKHQPDSHFYRKFVLYDNRIVGCILIGNINRAGIYNLLIR
                     EKIVVTAFKKDLLEESFGFLMLPREVRKHIVVGESMEV"
     gene            complement(1308..1778)
                     /locus_tag="PLW07_02925"
     CDS             complement(1308..1778)
                     /locus_tag="PLW07_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOL49508.1"
                     /translation="MVKNLQKKINTIVAKEEFCIGCRLCEIACITAHSKTKNIIKAFK
                     GEYPLPESSIVFEEKGYVSFALQCRHCDDALCVECCITGAMTKKDGRVIYRKELCVGC
                     WMCVMVCGFGVIAPDINTHKIKSKCDFCLETGKPACVEMCPNEALVLKETHEQD"
     gene            complement(1772..3274)
                     /locus_tag="PLW07_02930"
     CDS             complement(1772..3274)
                     /locus_tag="PLW07_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004083229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HOL49509.1"
                     /translation="MAIPKIVPDFKVIRDENLCINCQACCRVCANDTHSFDQNLMKII
                     SDHRKCVGCHFCEEICPTGALTIEKCYSQIRENFNWNEVDFKNLMKQAHSGGILLTGM
                     GNDRPWINYFDHIVLNASQVTNPSIDPLREPMEIKTFIGRKPDSVDQVKNKKHKFVLL
                     ETEIPVLFSAMSYGSISLNACKSIAAAAWEAKTYWNTGEGGLPESLKKFVSRTIVQVA
                     SGRFGVDAKYLKDAAAIEIKIGQGAKPGIGGHLPGEKVSAEISAARKIPEGTDAISPA
                     PHHDIYSIEDLKQLIYALKETVNYEKPVGVKVAAVHNIAPIVSGIVRAGADFVTIDGF
                     RGGTGAAPVVIRNNVGIPIEIAIAAVDQRLREEGIRNHASIIAAGGIRSSADVVKAIA
                     LGADAVYIGTATLVALGCHICQKCYTGKCNWGIATQDPYLTRRLNPEIGKRRLVNLLR
                     AWALEIKEMMGGMGINAIESLRGNRDQLRGIGLNDKELELLGIKHAGEAW"
     gene            complement(3249..4376)
                     /locus_tag="PLW07_02935"
     CDS             complement(3249..4376)
                     /locus_tag="PLW07_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012310605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase family protein"
                     /protein_id="HOL49510.1"
                     /translation="MNCKNIYQCSDDRKFHIPSGCGIAGLFNKDGSPVSGEKIFRAIN
                     SMKERGNGLGSGYAGYGIYPDYRNDWCFHLLYSQATARDQVEQYFKTFFHVKLSEKIP
                     TRKIKSIKNPPLLWRYFLQTKNSTPAEKEENLIVSVCMWINHNIQDAFVISSGKDMGI
                     FKGVGEPAEIADFYRLEDYKGYLWTGHNRFPTNSVGWWGGAHPFGLLDWSVVHNGEIS
                     SYGVNRRYLENFGYYCCLFTDTEVITYLFDLLVRKHNLSLDTIGNIISAPFWIQIDNY
                     SEQERYLLKWLRIIYGPALVNGPFSIIVARGNFMLGINDRIKLRPLVAAEKGNSFFLA
                     SEESAIVSMEKNPDNIWTPEAGAMTVGFFEKGNHGNSKNRT"
     gene            complement(4345..5667)
                     /locus_tag="PLW07_02940"
     CDS             complement(4345..5667)
                     /locus_tag="PLW07_02940"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HOL49511.1"
                     /translation="MTAKDVLKIVKENDVKFVRLWFTDLLGQVKSFAITNRELEGALE
                     NGMGFDGSSITGYQRIEESDMIAMPDPSTFAILPWRPKEHAVARMICDVMQPGGKPYE
                     GDPRFVLKKIIRKMKESGFSHYYVGPELEFFYLKNDRATEALDSGGYFDLTTLDIASD
                     LRRDTVLALENMGIKVEYSHHEVGPSQHEIDMRYEDALKMADNVITYRVVVKEIASMH
                     GVYATFMPKPIFGVNGSGMHTHQSLFTDDKNSFFDPKDQYHLSDVAKKFIAGQLTHAK
                     EICAIFAQWVNSYKRLVPGYEAPVYIAWSQRNRSALIRVPYYHPGKEQATRAEFRACD
                     PSCNPYLTFACLLGAGLAGIKNNYQLKDPIDKNLYHLSEVERKQMGIESLPGNLGESI
                     SLLEQSHLVKEILGEHIFSRFLKIKKQEWEDYRIQVTEYELQKYLPVL"
     gene            complement(5806..6501)
                     /locus_tag="PLW07_02945"
     CDS             complement(5806..6501)
                     /locus_tag="PLW07_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOL49512.1"
                     /translation="MERKNLQEQLEALTKISKAITSDLYLEDILKLIVLVTAELMGSN
                     ICSILLLDEKTKKLHIRATQSVSEEYNKKPPLSIGEGIAGKVVKENRSIVVKDVTKEE
                     EYKYRDIAVKEGLCSLLCVPLAIKNRVIGVINCYTSQPHDFTEAEIKILTTIANQAAV
                     AIENAELLVKSKVIQEELETRKLVERAKGILMKKENLTEEEAYRKLRKYSMDRRKTMK
                     EIAEAIILASDLI"
     gene            complement(6551..>6972)
                     /gene="carB"
                     /locus_tag="PLW07_02950"
     CDS             complement(6551..>6972)
                     /gene="carB"
                     /locus_tag="PLW07_02950"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF014225.2"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HOL49513.1"
                     /translation="LPVNGNIFISVQDRHKDLIYPVAKGFVELGFHVIATAGTGKFLS
                     EKGLSVTIIGKIYESVRPNVIDLIKNNQIQMIINTPSGTRPKMDIISIRTIAIHRNIP
                     LITTVRAAHATLLAIKKLREHQMQVKDIKQYHQMLET"
BASE COUNT         1871 a         1525 c         1259 g         2317 t
ORIGIN      
        1 ttttgcagca tcaatccttt ttttcatttc aaacctccat actttctcca acaacaatat
       61 gttttctcac ttccctgggg agcattaaaa atccaaagga ctcttccagc aaatcctttt
      121 tgaatgctgt caccacaatt ttttcccgta tcaacagatt ataaatccca gccctgttga
      181 tattgccaat taagatgcaa ccgacaattc tattatcata taaaacaaat ttgcggtaaa
      241 agtgactatc tggttgatgt ttttcaagta tttcaaaatt ttttgattga ggatttgtaa
      301 tcccaaaaga aatagcaggt agttcaaata tctgaattga gttcatagga tacagtcctg
      361 catacgaaat gttctgtcct gtcatattgg agccagcaac ttttcctgtt tttgcagcga
      421 ctggccagat tggaatgaga gcatttttat tttcaaaaaa atcgggtgcc tctgcaacat
      481 ctcctgctgc aaacacgtca gaaacatttg ttttcaaatt gttatccacc actattccac
      541 gatttactac gatggggctg tttttaacaa tagttgtcgc aggtcttacc ccaacagcaa
      601 tgataagaca ctctgtttca atttgtttgc cgcttttcag tatcactcgt ttgattttgt
      661 tgttttcact ttcaatccgg atgatagtat catttttgat aaattcagaa ttgtgtctgg
      721 caagatatga ttgaatgatt tcagagcctt tgacatccat tgtatttgga agaaccctgt
      781 cagccatctc aatgattgtt acaggaatgt tcctcatcaa cagtccttcg gctgccttca
      841 gacctatgag acctgctcca aggattacac tattgtctgg attgacagat tcaatgtatt
      901 gtgcaagaga tatggcatca ttgagtgaat aaaaagtgaa aataccttct aattggtttg
      961 ataacccgtc aatcggcgga aaccatggtt ttgcgcctgt cgcaatcaaa agtttttgat
     1021 acctgatttt cttccctgca ggaagttcaa ttgcatgatt ggaacagtca agagaaatgg
     1081 caggattatt aataagcaga tgcgccttca tgtctgaata aaaagattca tccctgaacc
     1141 tggtttgctg ttgtgagatt tttcgcccaa gaaagtatga aatcaatggt cttgagtaat
     1201 tttcaaacgg ttcctctgaa accacaagaa tttcgccttt tgagtcatat tcacggatac
     1261 cttctatggc attgacacca ctgacagaat tgccaataat gagataatca gtcctgttca
     1321 tgggtctcct ttaacacaag tgcttcattt ggacacattt caacgcatgc cggttttcct
     1381 gtttcaaggc aaaaatcgca ttttgatttt attttgtgag tattgatatc aggagcaata
     1441 actccaaacc cacacaccat tacgcacatc cagcaaccta cgcacagctc ttttctgtat
     1501 atgaccctgc catctttttt tgtcattgct ccagtgatac aacattcaac acacaacgca
     1561 tcatcacaat gcctgcactg aagagcaaaa gatacatacc ctttttcttc aaaaacaatg
     1621 gaagattctg gaagaggata ctctcctttg aatgctttga taatattttt tgtttttgag
     1681 tgtgcggtaa tacaggcaat ttcacacaac cgacaaccga tacagaattc ttcctttgca
     1741 acgattgtat taattttctt ttgcaaattt tttaccatgc ctctcccgcg tgtttgatac
     1801 caagaagttc cagttcttta tcatttaatc caatgccacg aagctgatct ctgttgcccc
     1861 tgagagattc aatcgcattg attcccattc ctcccatcat ttctttaatt tccagtgccc
     1921 atgctctaag taaattgaca agccttcttt tgcctatttc aggattcaac cttcgggtaa
     1981 gatatgggtc ctgtgttgct attccccagt tgcacttgcc tgtatagcat ttctggcaaa
     2041 tatggcaacc aagcgcaaca agtgtggcag ttcctatgta tacagcatca gctcccaggg
     2101 caatggcttt taccacatca gcgcttgatc tgataccacc agcagcaata atggatgcat
     2161 ggtttcttat cccttcttct ctcaatctct ggtcaactgc agcgatggca atttcaatcg
     2221 ggattccgac attgtttctg atgacaaccg gcgctgctcc tgtaccgcct ctgaatccat
     2281 cgattgttac aaagtcagca cctgctctta caatgccact tacaatgggc gcaatattat
     2341 gcacggcggc aaccttgaca ccaacgggtt tttcatagtt gacggtttct tttaatgcat
     2401 aaatcagttg ttttaaatct tcaatggaat aaatgtcatg atgtggagca ggcgagatgg
     2461 cgtcagtgcc ttctggtatt ttgcgcgcag ctgaaatttc agcgcttacc ttttccccag
     2521 gaaggtggcc tcctatgcct ggctttgccc cctggcctat ctttatttct attgctgcag
     2581 catcttttag gtacttagca tccacgccga atctaccaga tgcaacctgt acaatcgttc
     2641 ttgaaacaaa ttttttcaaa ctttcaggca atccaccttc acctgtattc cagtatgttt
     2701 ttgcttccca tgcagcggca gcaattgatt tacacgcatt gagtgaaatt gatccgtaac
     2761 tcattgcaga aaataacacg ggtatttcag tttcaagcag aacaaattta tgtttcttgt
     2821 tcttcacctg gtcaacgcta tctggttttc tgcctataaa agtttttatt tccattggtt
     2881 cacgcaatgg gtctatggaa ggattggtaa cctgtgatgc gttaagaaca atatggtcaa
     2941 aataattaat ccagggtcta tcattgccca tacccgttag cagtattccg ccagaatgtg
     3001 cctgtttcat taaatttttg aaatccacct cgttccagtt gaaattctca cgaatctgcg
     3061 aataacattt ttcaatggtc agagcacctg tgggacatat ctcctcgcag aaatgacatc
     3121 ccacacactt cctgtggtct gaaatgattt tcatgaggtt ttgatcaaaa gaatgggtat
     3181 cattggcaca taccctgcag catgcctggc agttgataca gagattttca tccctgataa
     3241 ctttaaaatc aggtacgatt tttggaattg ccatgattcc ccttttcaaa aaaaccgacg
     3301 gtcatagcgc ctgcttctgg tgtccagatg ttgtcaggat ttttttccat gctgacaatt
     3361 gcggattctt ctgaagcaag aaaaaaacta ttaccttttt ccgcggcaac aagaggtctt
     3421 aatttgattc tgtcattgat gcccagcata aaattacccc ttgccacaat gatactgaaa
     3481 ggtccattga caagagctgg gccatatatg attctgagcc atttcaaaag gtatcgctcc
     3541 tgttcagaat aattgtctat ctgtatccag aatggcgctg atataatatt tccaatggtg
     3601 tctaaggata ggttgtgttt tctaacaaga aggtcgaata gatacgtgat gacttcagta
     3661 tcagtgaaca ggcaacagta atatccaaaa ttttcaagat atcttctgtt tacaccatag
     3721 gaagatattt ctccattatg aaccactgac caatccagta gaccaaatgg atgcgctcct
     3781 ccccaccatc caacagaatt ggtaggaaac ctgttgtgcc ctgtccatag atatcctttg
     3841 taatcttcca atctgtaaaa atctgctatt tcggctggtt ctcccactcc tttaaaaatt
     3901 cccatatcct tgccagagga aattacaaaa gcatcctgta tattgtgatt aatccacata
     3961 cacacagaaa caatcaggtt ttcttctttt tcagcaggcg tactgttttt tgtttgcaag
     4021 aaatatcgcc agagcaaagg aggattcttg attgatttga tttttcttgt gggtattttt
     4081 tctgaaagtt tcacatggaa aaaagtttta aaatattgtt ccacctggtc tcttgcagtg
     4141 gcctggctat aaagcaaatg aaaacaccaa tcatttctgt aatcaggata tatgccataa
     4201 ccagcgtagc cactgccgag accattgcct ctttctttca tcgagttgat tgccctgaaa
     4261 atcttttcac ctgaaacagg gctgccgtct ttattaaata atccggctat tccacaccct
     4321 gatgggatgt gaaattttct gtcatcagag cactggtaga tatttttgca gttcatactc
     4381 tgtcacctga atcctgtaat cttcccattc ttgtttttta attttcaaaa acctgctaaa
     4441 aatgtgttca ccaagaattt cctttaccag atggctttgt tcaagaaggg aaattgattc
     4501 accaaggttt ccgggtaaac tttctattcc catctgtttt cgttcaactt cagaaagatg
     4561 ataaaggttt ttgtcaattg gatctttcaa ttgataatta ttcttaatac ctgcaagccc
     4621 tgcaccaaga agacaggcaa aggttaaata tggattgcag gaagggtcgc aggctctgaa
     4681 ttctgccctg gttgcctgtt ctttacctgg atggtaatat gggaccctga ttaaagcaga
     4741 cctgtttctc tgtgaccatg cgatataaac aggtgcttca tatcctggca ccagtcgttt
     4801 gtaagagttc acccattgtg caaagatcgc acatatttct tttgcatggg tcagttgccc
     4861 tgcaatgaat ttttttgcga catccgaaag atgatactgg tcttttggat cgaaaaatga
     4921 atttttgtca tcggtaaaaa gagactgatg ggtatgcatg ccacttccat taacaccaaa
     4981 aattggtttt ggcataaagg ttgcgtaaac gccgtgcata cttgcgattt ccttaacaac
     5041 taccctgtag gtaataacat tgtcagccat tttcaaagcg tcttcatatc tcatatctat
     5101 ttcgtgttga gatggaccaa cctcatgatg cgagtattca acttttatgc ccatgttttc
     5161 aagagcaagc actgtatctc ttcgcaggtc tgatgcaatg tcaagagtgg taagatcaaa
     5221 atatccgcca gagtccagcg cttcagtggc tctgtcgttt ttcaggtaga agaattcaag
     5281 ttctggacca acatagtaat gggagaatcc ggattccttc attttcctga ttatcttttt
     5341 aaggacaaac cgtgggtctc cctcgtatgg ttttccgccg ggttgcatga catcacatat
     5401 cattcttgcg acagcatgtt cttttggcct ccatggaagt atcgcaaacg ttgaagggtc
     5461 aggcatcgca atcatatctg attcctcaat tctttgatag ccggtgattg acgagccatc
     5521 aaaccccatc ccattttcca gggctccttc aagctctctg tttgtgattg caaaactctt
     5581 tacctgtcca agaaggtcag tgaaccacag cctgacaaac tttacatcat tttccttcac
     5641 aatcttcaat acatcttttg ccgtcattta accccccttt ctgttaaaat aaaaaagtcc
     5701 tttccggcac tcgacgccga aaaggacttc agtgtccaac cttcgcacag cttcgtacga
     5761 atcaaatttt attatgataa taccacaact ttttttctgt caaatctata tcagatcaga
     5821 agcaagaata atggcttcgg ctatttcctt cattgttttg cgcctgtcca tgctgtactt
     5881 tctgagtttc ctgtatgcct cttcttcagt aaggttttcc tttttcatca gaatcccttt
     5941 tgctctttca acaagttttc ttgtttcaag ttcctcctga atgaccttgc ttttcaccag
     6001 taattctgca ttttcaattg ccactgctgc ctgatttgcg attgtggtca agattttgat
     6061 ttctgcctct gtaaaatcat gcggttgtga ggtgtagcag ttgatgactc cgattacacg
     6121 atttttgatt gcaagtggca cacacagaag agagcagaga ccctctttta cagcaatgtc
     6181 cctgtattta tattcttctt cctttgtaac atctttcaca acaatagatc tgttttcttt
     6241 cacgactttt ccagcaatgc cttcgcctat acttaatggt ggttttttat tatattcttc
     6301 actgacactc tgtgtcgctc tgatatgaag tttttttgtt ttttcatcaa gcaaaagtat
     6361 ggaacagatg tttgaaccca tcagttccgc agtgacaagc actatgagtt taagaatatc
     6421 ttcaaggtac aaatcagagg taatcgcttt gctgattttt gttaatgctt ccagttgttc
     6481 ctgaaggttt tttctttcca taggatgtgc gcctttttgt gataatttat aattttttta
     6541 gaaagaactg tcaagtttcc agcatctgat gatattgttt aatatctttc acctgcatct
     6601 gatgttccct taattttttt atcgcaagca acgttgcatg tgctgccctt actgttgtga
     6661 tgagaggaat gtttctatgt attgctattg tcctgataga aataatgtcc atttttggcc
     6721 ttgtacctga tggagtattg ataatcattt gaatctgatt gttttttatc aagtcaatga
     6781 cattgggtct tactgattca taaatcttgc caatgattgt tacactaaga cctttttcag
     6841 acaaaaattt tcctgttcca gcagttgcaa taacgtgaaa cccgagttca acaaagccct
     6901 ttgcaacagg ataaatcaaa tctttgtgtc tgtcctgaac actgatgaaa atatttccat
     6961 tgactggtag at
//
LOCUS       DAORYU010000058         6869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_7761_length_6869_cov_5.171118, whole genome shotgun sequence.
ACCESSION   DAORYU010000058 DAORYU010000000
VERSION     DAORYU010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6869
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7761_length_6869_cov_5.171118"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="PLW07_02955"
     CDS             complement(<1..783)
                     /locus_tag="PLW07_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49514.1"
                     /translation="MLVENPLYIALKGIQTRPLRILSLIAAEIILIGIVYFFISIGHP
                     YTKLSSTQISKLIGEEFAIAMLVIQYIVLVIYGTFSIETRIVRDRMSDIINFHRLTPL
                     SPQNIISGYIFGQSSDAIIIWGISFLAGIIGVLSGGISFYQYFSSSILICMCAAFFYL
                     LATWSGLTKSLTLKFGARQTWGFATVLTFLAVNPILINLSPIPAIRTIINDQSNFYPN
                     TYTAFVYFYGSPIPTIFYTMMLWITFGTIAFFSSKRKIFSEDS"
     gene            complement(773..1711)
                     /locus_tag="PLW07_02960"
     CDS             complement(773..1711)
                     /locus_tag="PLW07_02960"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOL49515.1"
                     /translation="MEAIVNVENLIVDFENVTACKNISFHVFTGDIFGLVGPNGAGKT
                     TTLKVLATLLKPTHGKITINGFDLEKQTLQIRKTIGFMPDFSPVYEEMKVWEFLELYA
                     SAYGIETYKRKERIESELHMVNLMTKKDAFIGELSRGMKQRLVLAKTLLPQPQILLLD
                     EPASGLDPLARKELSEILQNLGNEGKTIIISSHILTELADFCTAAGIMEKGKMIKWGK
                     LENLSDIPADTVCVELNTFKNLHDLTKILTNYPSLLKFEKKSNEKWHIYIKGNDSTIP
                     DFVKFLVESNVPILEIKKIKEDIQTIFFKTSAKNVS"
     gene            1779..4376
                     /locus_tag="PLW07_02965"
     CDS             1779..4376
                     /locus_tag="PLW07_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49516.1"
                     /translation="MKSKIILYLIFCVTCACFAGVNMGGFIIDIQTQLPVSDATVSIV
                     GTNLSATTSQTGFFLISNIPVQSTGFQFKASKAGYVDTYTMLGNAGTTNVSEVSFPVF
                     SNTMYSNMHSVAFGGIAHTSGKADIIGSVDSSDKAGVSEVVISAKYMDTNANAGTIRY
                     VNTNSMPDSSLTSTSSSGAFIIYNVDPYRPIKIIGTKAGSVFSSSIVIGYPDKITVGG
                     IEQVSNLLQLSAKVLWDENPVSGATVSIPGTTTTTTSAADGSFTLDVNPTSYGVLKIS
                     KTNYIDSYFTGRADEEGRKQNGDDEEEFFAIPVSDYNSILGSLGKSHVSGKGDIIGEV
                     LTINYFSVAGATISVYDRNGTKLNPDIIYFNEEGEPDISLTSTTSDSNFLVLNLDPGY
                     YFITAEKSGFEFSRNMAVVFANGITSPPEIISYPPLPGIAKAKAEDIPSANVSKSAEN
                     VKMLAFMLNLMRSGTDESVIFDSAIVTAKGTGNISTSLSSARLYLDSNNDGSYETLVS
                     TGIISGNKITFNNINKEVDMGLNQRYLVVFNFNGSASIGQTFGVDILKNSDVSAHAKD
                     SGLHVTCEGDEPVVGNLMTIANVYPPVQPVNISPENGATGIMPFNYVLQSSAFNAGQG
                     SNTHKASQWRIWKDGETQELFTWYGFEDQINLTTIHTPVPLEGLTKYWWQVRHENGDN
                     AWSDWSNPTNFTTDQGGITRPTKPSNQSPSDGEEDVSQPVTLTASAFVSGTHPQHVAS
                     QWQVFLATKNGGPVIDFDTGRDTNNLTSITLPLLLGNSPYMWRVRYQDGYGAWSQWSD
                     YTLFTTRQGMKGDLNNDNNVDISDVILCLRMAIGLDPINLYPADMNNDQQVDIADVIL
                     ILRKAIGLD"
     gene            complement(4394..5314)
                     /gene="cysK"
                     /locus_tag="PLW07_02970"
     CDS             complement(4394..5314)
                     /gene="cysK"
                     /locus_tag="PLW07_02970"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013195275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOL49517.1"
                     /translation="MKIFNDITETIGNTPLVKLNRITEGINATVLAKLESFNPLSSVK
                     DRIGVAMIDDAEKKGILNKNSVIIEPTSGNTGIALAFVAAAKGYKLILTMPETMSIER
                     RKLLAIFGAELVLTPGSEGMTGAVKKAEQLAKEIPNSVILQQFKNPVNPEIHRKTTAE
                     EIWNDTEGKVDILVSSVGTGGTITGIAEVIKKRKKTFKAIAVEPDASHVLSGGNPGPH
                     KIQGIGAGFIPDVLNLKIIDEIIRVKEQDAGETARKLAKLEGILAGISSGAACWAAIE
                     VAKKKESEDKVIVVILPDTGERYLSTWLFE"
     gene            complement(5629..6297)
                     /locus_tag="PLW07_02975"
     CDS             complement(5629..6297)
                     /locus_tag="PLW07_02975"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOL49518.1"
                     /translation="MNEINERKGMPLLGDKFPEMTVQTTHGVMVLPKEFSGKWFVLFS
                     HPADFTPVCTTEFVAFQKRYEKFKALNCELIGLSVDQVFSHIKWEEWIAENLKVEIQF
                     PIIADTGAVAEKLGLIHPGKGTNTVRAVFVVDAEGIIRIILYYPQELGRNMDEILRAV
                     EAMQISDKNKVAMPANWPNNEIIGDHVIIPPAKDVKTKKERLEMAKKGECKCFDWWLC
                     HKKL"
     gene            complement(6522..6592)
                     /locus_tag="PLW07_02980"
     tRNA            complement(6522..6592)
                     /locus_tag="PLW07_02980"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6558..6560),aa:Gln,seq:ctg)
     gene            complement(6604..>6869)
                     /locus_tag="PLW07_02985"
     CDS             complement(6604..>6869)
                     /locus_tag="PLW07_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HOL49519.1"
                     /translation="FSYMAGGLKDFFDRTFYPVQGKVTDKPCVIFVTHGGGGKAVDSL
                     ESMCKWFKFKKVAEPVLIKNKPSSSDESRLMELGAILVSAIKK"
BASE COUNT         2042 a         1298 c         1300 g         2229 t
ORIGIN      
        1 ggaatcttca gaaaatattt ttctttttga tgagaaaaat gcaatggtgc caaaggtaat
       61 ccacagcatc atagtgtaaa aaatagtggg tattggggaa ccataaaaat atacaaatgc
      121 tgtgtatgtg tttggataga agttgctctg gtcattgatg atcgttcgaa tagcaggaat
      181 cggcgaaaga ttaattaaaa ttggatttac agccaagaag gttaacactg ttgcaaatcc
      241 ccaggtttgt cttgcaccaa attttaatgt caatgatttg gtaagtccgc tccatgttgc
      301 cagcaaataa aagaatgctg cacacataca aatcagaata ctgctggaaa aatactgata
      361 aaaacttatc ccacctgaca aaacgccaat gatacccgca agaaatgaaa tgccccagat
      421 aatgattgcg tctgatgatt gaccaaatat gtatccagag ataatattct ggggggaaag
      481 aggagtcaga cgatggaaat taataatgtc agacattctg tccctgacaa ttcttgtttc
      541 tattgagaat gttccataaa tcacaagaac aatgtattga atgacaagca tcgcaatagc
      601 gaactcttcg ccaatgagtt tagaaatttg agtactactt aattttgtat atggatggcc
      661 gatcgagata aaaaaataca caattccaat aagaataatt tctgctgcaa tcagtgacag
      721 tattcttaat ggtcttgttt gaattccttt taatgctatg tacaaaggat tttcaactaa
      781 cattttttgc acttgttttg aaaaaaattg tttgaatatc ttctttgatc ttttttattt
      841 ccagaatcgg gacattgctc tctaccagaa attttacgaa gtcaggtatt gtggagtcat
      901 ttcctttgat ataaatgtgc catttctcat tactcttttt ttcgaatttc agcagagaag
      961 ggtagtttgt caaaattttt gttaaatcat gtaaattttt aaatgtgttt agttccacac
     1021 agacagtgtc agccggtatg tcagaaagat tttcaagttt gccccattta atcattttcc
     1081 ctttttccat tattccagca gcagtacaga aatcagccag ttcagtcaaa atatgagacg
     1141 aaataataat tgtttttcct tcgttaccaa ggttttgcaa aatttctgaa agttcttttc
     1201 ttgcaagggg atcaagtccg cttgctggtt catcaagcaa aaggatctgt ggttggggaa
     1261 gaagggtttt tgcaagaaca agccgttgtt tcatacccct tgacagttct ccaataaaag
     1321 catccttttt tgtcataagg ttgaccatgt gaagttctga ttcaattctt tcctttcttt
     1381 tgtatgtttc aataccataa gcagaagcat aaagttcaag gaattcccag accttcatct
     1441 cttcataaac aggtgaaaaa tcaggcataa agccgatggt ttttcttatc tgaagagttt
     1501 gtttttcaag gtcaaatcca ttgatagtaa tttttccatg tgttggcttc agaagggttg
     1561 ccagaacttt taatgttgtg gtttttcctg ctccatttgg gccaacaagg ccaaaaatgt
     1621 ctcctgtaaa tacatgaaaa ctgatatttt tgcaggcagt gacattttca aaatccacaa
     1681 tcaaattttc tacattgaca attgcttcca tttgtataga ataatataat aattctggaa
     1741 attgttcaat attgtgtaca gagaggaggg aaggggaaat gaaaagtaaa ataatacttt
     1801 atctgatatt ctgtgtaacc tgtgcttgtt ttgctggtgt gaatatgggt ggattcatca
     1861 ttgatataca aacacaacta ccagtatcag atgccactgt ttcaattgtg ggaacaaatc
     1921 tttctgcaac aacttctcaa actggatttt tcctgatttc taatataccg gtgcagtcaa
     1981 caggttttca attcaaagca agtaaagcag gttatgtgga tacttataca atgcttggca
     2041 atgctggaac tactaatgta tcagaagttt cattccctgt tttttcaaat accatgtaca
     2101 gcaacatgca tagtgtggct tttggcggaa ttgctcatac atcaggcaaa gcagacataa
     2161 ttggtagtgt agacagtagt gataaggcgg gtgtaagtga agtggtgatt tctgcaaaat
     2221 atatggatac caatgcaaat gctggaacta ttagatatgt aaatacaaat agcatgcctg
     2281 acagttcttt gacatccacc tcatcaagtg gtgcattcat tatttataat gttgaccctt
     2341 atagacccat taaaattatt ggaacaaaag caggaagtgt tttttcttcc agtattgtga
     2401 ttggttatcc tgacaaaatt acagttggtg gtattgaaca ggttagtaat cttcttcaat
     2461 tatctgcaaa ggtattatgg gatgaaaatc cagtgtcagg agcaacggtt tcaattcctg
     2521 gtactacaac cactaccaca tcagcagcag atggttcttt cacactggat gttaatccaa
     2581 catcctacgg ggttctcaaa atttcaaaaa cgaactatat tgattcatat tttacaggca
     2641 gggctgacga agaaggtcga aagcaaaatg gagatgatga agaagaattt tttgcaattc
     2701 ctgtatctga ttataattct attctgggtt ctcttggtaa aagtcatgta tctggaaagg
     2761 gagatattat aggagaagtt ctcaccatta attacttttc tgtggcagga gcaacaatca
     2821 gtgtttatga tagaaatgga accaaattga atcctgacat tatttatttt aatgaagaag
     2881 gagaaccaga tatttccctt acttcaacaa catctgatag taattttctg gtattaaacc
     2941 ttgatcctgg atattacttt atcacagcag aaaaatcagg ttttgagttt tcgagaaaca
     3001 tggcagtcgt gtttgccaat ggcattacca gtcctcctga gattatttca tatccaccgc
     3061 ttccagggat agcaaaagca aaagcagaag atattccatc agccaatgtt tcaaaaagtg
     3121 ccgaaaatgt taaaatgctt gcttttatgc ttaatttgat gaggtcgggc actgatgaaa
     3181 gtgtaatttt tgatagtgcc attgtcacag caaaaggaac aggtaatatt tccacatccc
     3241 tttcaagcgc aagactttat cttgactcaa acaatgatgg aagttatgaa acactggttt
     3301 caacaggaat tatttctggc aataaaatta ctttcaacaa tatcaataaa gaagttgata
     3361 tgggattaaa tcagagatac cttgttgtgt ttaatttcaa tggaagtgct tcaattggtc
     3421 agacatttgg tgttgatata ctgaaaaatt ctgatgtatc tgcgcacgca aaagattctg
     3481 gattacatgt aacctgtgag ggggatgaac ctgttgttgg taatttaatg accattgcga
     3541 atgtgtatcc ccctgtacaa cctgtgaata tatcgcctga aaatggtgcc acaggaatta
     3601 tgccatttaa ctatgtgctt caatcaagtg ctttcaatgc cggtcagggc agcaataccc
     3661 acaaggcgtc tcagtggaga atctggaaag atggagaaac acaggaatta tttacctggt
     3721 atggttttga ggaccagatt aatctaacta caattcatac acctgtgcca cttgaaggat
     3781 tgacaaaata ctggtggcag gtgagacatg aaaatgggga taatgcctgg agcgattggt
     3841 ctaacccaac aaatttcaca acagaccagg gtggtatcac ccgaccaacc aagccatcaa
     3901 atcaatcgcc atctgatgga gaagaagatg tttcacaacc tgtgacattg actgcaagcg
     3961 catttgtttc aggtactcac cctcagcatg tggcatctca gtggcaggtt tttcttgcga
     4021 caaaaaacgg tggacctgtg attgattttg ataccggaag agatacaaac aatcttacat
     4081 caatcacatt accattactt ctggggaact caccgtatat gtggagggtg agatatcaag
     4141 atggttatgg tgcatggagc cagtggagtg attacacact ctttacaaca aggcagggaa
     4201 tgaaaggcga cttgaataat gataacaatg ttgatatcag tgatgtaata ctctgtttga
     4261 gaatggcaat aggccttgac cctatcaatc tatatcctgc ggacatgaat aatgaccagc
     4321 aggttgatat tgcagatgtg attctaattt tgagaaaagc aattggcctt gattaatttt
     4381 tttatccagt cacttattca aaaagccatg tagaaagata tcgttctcct gtgtcaggaa
     4441 gaatcacaac gattactttg tcttctgatt ctttcttttt tgcgacttca attgcagccc
     4501 agcaggcagc cccacttgaa attcctgcaa gtataccttc aagttttgca agttttcggg
     4561 cagtttcacc agcatcctgt tctttcacac gtatgatttc atcaatgatt tttaaattaa
     4621 gtacatcagg aataaatcct gcgccaatac cctgaatttt atgaggacca ggattacccc
     4681 ctgataatac atgagaagca tctggttcaa ctgcaatggc tttgaacgtt tttttccttt
     4741 tttttatcac ttctgctatg cctgtaatgg ttccgcctgt tcctacacta ctaacaagaa
     4801 tatcaacctt tccttctgta tcattccaga tttcttcagc ggttgttttc ctgtgtattt
     4861 ctggatttac aggattttta aattgttgca aaatcacaga attaggaatt tcttttgcca
     4921 gttgttctgc tttttttaca gcgcctgtca tcccttcgct tccaggggta agtaccaact
     4981 ctgcaccgaa aatagcaagt aattttcttc tttcaatgct cattgtttct ggcattgtaa
     5041 ggatgagttt atatcctttt gctgcagcaa caaaagccag tgcaatacct gtgttaccac
     5101 ttgtgggttc tatgataaca gaatttttat tgagaatacc ttttttttca gcatcatcaa
     5161 tcattgcaac accgattcta tcctttacac tggacagtgg attgaatgat tcaagttttg
     5221 ctagtactgt agcattgata ccttcagtaa tgcggtttaa ctttacaagc ggcgtattgc
     5281 ctattgtttc tgtaatgtcg ttaaaaattt tcatattttc ttctccatgt tcgaatattc
     5341 aagcggtttc aggatgtttt cttttattct acaccatgaa tcaagaacaa tacaatttcg
     5401 ggcttctggg acattttgta tggttaattg ctagcaaata gtaatagcag caactattgg
     5461 ttgaacaggt ttttctccat agtaagtgtg aatatcgtgg tatcaaaact tggttaaatt
     5521 atgttttgcc ttgaagaaat ttttcaaaag ccaaaacaca ttacagaaaa tgataccatt
     5581 cggcattttg tgtgttaatt atagacctga aatccagata ttcaggtatt acaatttttt
     5641 atggcatagc caccagtcaa aacacttaca ttcgcctttc ttagccattt caagtctttc
     5701 tttttttgtt ttaacatctt ttgctggcgg gataataaca tgatcaccga taatttcatt
     5761 gtttggccag ttggctggca ttgccacttt gttcttgtca gagatttgca ttgcctcaac
     5821 agcgcgcaga atttcatcca tgtttctgcc gagttcctgc ggataataca gtataattct
     5881 gataatccct tcggcatcaa ccacaaatac tgctcttact gtatttgtac ctttgcctgg
     5941 atgaataagt ccaagttttt cagcaacggc tccagtatct gcgatgatgg gaaactgaat
     6001 ttcgactttc aagttttcag caatccattc ttcccatttt atatgtgaaa aaacctgatc
     6061 aacactaagt ccgatgagtt cgcagtttaa tgctttgaac ttttcgtatc ttttttgaaa
     6121 agccacaaat tcagtcgtgc aaacaggagt gaaatctgct ggatggctga aaagcacaaa
     6181 ccatttacca gaaaattctt ttggaagcac cataacacca tgcgttgttt gaacagtcat
     6241 ttcaggaaat ttgtctccaa gcaacggcat accttttctt tcatttattt cgttcatttt
     6301 cctcctttgt aaggggttgt gtatatcatt tatctggggg actaggattt ggcgccctcg
     6361 tctcccatta cgactcgggt cgatccggcc tgcctcaccg actcggcagg cttgccgtac
     6421 aatgctcttt attcatcgca ttggcaacca accgcgctgg tcttcgcttg ctcagaccag
     6481 tacgccttcg ctccattcaa atcctttttg aaaaaaatta tctgggggac taggatttga
     6541 acctagacac tcagatccag agtctgatgt cctaccgtta gacgatcccc catgttgaat
     6601 tttctatttt tttattgctg aaacaagaat tgcgccgagt tccatcagcc tgctttcatc
     6661 agaagatgat ggcttatttt tgatcagtac tggttcagca actttcttga atttaaacca
     6721 tttacacatt gactcaagac tgtctactgc cttgcctcca ccgccatgcg ttacaaatat
     6781 aacacagggt ttgtctgtta ctttaccctg tacaggataa aaagttctat caaaaaaatc
     6841 cttcaatcca cctgccatat agctgaaat
//
LOCUS       DAORYU010000059         6805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_7825_length_6805_cov_4.615407, whole genome shotgun sequence.
ACCESSION   DAORYU010000059 DAORYU010000000
VERSION     DAORYU010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6805
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7825_length_6805_cov_4.615407"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="PLW07_02990"
     CDS             complement(<1..473)
                     /locus_tag="PLW07_02990"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOL49520.1"
                     /translation="MKISEFISEKEILIGLKATQKQEALDELLQVLENNGLLQERKQV
                     LEALMEREKFGSTGIGQGIAVPHAKTDKVNNLVCALGISDKGVDFDSLDGESVYIIFL
                     VLAPSGATGIHLKALAKIARLLKDKVFRNYLRMSKTSQEAYQIIKDDEERLNSTG"
     gene            complement(470..829)
                     /gene="raiA"
                     /locus_tag="PLW07_02995"
     CDS             complement(470..829)
                     /gene="raiA"
                     /locus_tag="PLW07_02995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOL49521.1"
                     /translation="MIFHLQPVHVHLDSGFEQLAEEKFSRLERFVSGEPEVHLIVKKE
                     KFEFIFEAKIRYRSRVIFMKTSSNNLNAGLDDLINKLKNHLSKLHDKKHNKKNRKSGE
                     IEFFSSTVNNETEEEQT"
     gene            complement(844..1872)
                     /locus_tag="PLW07_03000"
     CDS             complement(844..1872)
                     /locus_tag="PLW07_03000"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOL49522.1"
                     /translation="MSRFVSKKKLLAAISIFLLNLTLCCAETVRICIWEGSQRPVLSS
                     FRGKYRGQLSLVKWNKKYLVINTMDVEEYLFGVVGKEMEKSWPFEALKAQAVCSRTLI
                     YHFKDLAMKKNIPYDVSDTIHHQVYGGVISENEAIVRAVEETSSQVLVGQGEEKGYNI
                     IPTFFHACCGGHTTSAEDIWGGKYLLLNGVEDPYCEGTPFYAWSKTYTRDDVSSIVGT
                     DVNTINLAGFDSSGRIKRIEFSGKFGKKVISAENFRMLTINSNTTFKSARTLPSTMFT
                     VSKNGNNFVFSGKGYGHGVGMCQWGARRMAEDGKNYVEILRHYFPGLVLVSLNQTNVS
                     SSVVIEKP"
     gene            complement(1829..2401)
                     /locus_tag="PLW07_03005"
     CDS             complement(1829..2401)
                     /locus_tag="PLW07_03005"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HOL49523.1"
                     /translation="MAKSAGKIVGILAVVLLMISGCTRKKPTLETQETGQILKNFKLE
                     FFDSSFKITLEGLAAEKKQDSHTAVSQPAIEIKSKNFILEIRTGSKGTGELFLDPSTQ
                     AVMKIVIQNNVMITQKNPENSQTNFSATCEKLTYIEKDDTVVMEGSPQVIQGSNLYSA
                     DRITYTFKENKLRFEGNVQVRFKKETAGSN"
     gene            complement(2365..2877)
                     /locus_tag="PLW07_03010"
     CDS             complement(2365..2877)
                     /locus_tag="PLW07_03010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HOL49524.1"
                     /translation="MDVCRIKLVATDVDGVLTSGGIFYSGVANQGKFFSVRDGFAFKL
                     LKLAGLRSVIISGKKTGILKNRFSDVPVDSIYENVDNKMAVMRKICEKECLAMEEVCY
                     IGDDILDIDVLRNVGFSVAPRDADEEVKNVVMYVTSVNAGKGVFRECVEVILKGQGKW
                     QKVLEKSLEF"
     gene            complement(2879..3874)
                     /locus_tag="PLW07_03015"
     CDS             complement(2879..3874)
                     /locus_tag="PLW07_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HOL49525.1"
                     /translation="MKTNFSERKILTAAKRVIDIEVSNLLRAKKHLGSEFVKAIRMLA
                     SCKGKVIVTGIGKSGIIGRKISATLASTGTPSTFLHPGEAIHGDLGMVCGSDVVVAIS
                     NSGETDEILKIVPSLKKIGTPIISITSDARSSLGLQSTIVLNTGTIQEADPFSLIPSS
                     SATTALVLGDAMAITLLSVKGFQRQDYAFYHPGGNLGRRLMLKVKDVMQTGKKIPVVD
                     SERTMEDAIIEINRKNLGFTLVTDRKSRVIGIITDGDLRRLLAKKKNVFSCKVLECMT
                     PNPLGIEEEKLAVQALAIMEEKEITCLVIRDKIGKAKGIVHLHDLLGKRHFSQEF"
     gene            complement(3855..4700)
                     /gene="kdsA"
                     /locus_tag="PLW07_03020"
     CDS             complement(3855..4700)
                     /gene="kdsA"
                     /locus_tag="PLW07_03020"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017951875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HOL49526.1"
                     /translation="MNIVRIRDCVVGGSKLCIIAGPCVLENETLVLKVAESMHSLTQK
                     FNMSYIFKGSFDKANRSSIHSYRGPGLEQGLRILESIKNTIGVPVLTDVHCVTQVKPV
                     SEVVDVIQIPAFLCRQTDLLVSAAKSGKPVNVKKGQFMSPWETGNIVEKLTSSGCKKI
                     LITERGTSFGYNNLVVDFRSLPVMRNYGWPVVFDGTHSVQQPGAMGKSSGGQKEFVPY
                     LCRAAVAVGCDAIFLEVHPEPAKALSDGPNMVALSSMKYLLDELGKIWNTVIKIREKY
                     EDKLQ"
     gene            complement(4713..5060)
                     /locus_tag="PLW07_03025"
     CDS             complement(4713..5060)
                     /locus_tag="PLW07_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HOL49527.1"
                     /translation="MNQDCVFCRISEGKNPARLVYESEDIIAFHDINPQAPVHLLIIP
                     RRHFTNLLNVNEQDSTILAKMLFVATEIAKQTKIAETGFRIVINTNRNAGQSVDHLHI
                     HVLGGRIMEWPPG"
     gene            complement(5057..>6805)
                     /gene="infB"
                     /locus_tag="PLW07_03030"
     CDS             complement(5057..>6805)
                     /gene="infB"
                     /locus_tag="PLW07_03030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOL49528.1"
                     /translation="STGTETVGEIASKLGISAAEIIKNLSDIGISAAINDRLGPENIQ
                     KLCDIIGVSVQFKKTRESYILGTAVETDNDLVPRAPVVTVMGHVDHGKTTILDYIRKS
                     NVAAKEFGQITQRIGAYKVNLKEGSIVFIDTPGHEIFTAMRSRGAQITDIVVLVVAAD
                     EGVKLQTVEAINHCKAAGVPVIVAINKIDRPNANPDIVKKKLSEYGLVPEEWGGQTVF
                     LNVSGVTGANIKELLEIILLMGEMMELKANPERPGEGVVLEAYLHKQKGPVIDLLVQN
                     GSVCTGEFFVCGESWGKVRAMFDENGKRIEKAGPSTPVELLGASSTPVPGDLFKVVDS
                     EADARRIAEETKQANKSTGEKVQKVLTLEDLQKQIIQGTLKELKIILKTDSIGSQEAI
                     KSSLEKLSQSSVEKQGPSVNLLHCGLGSISESDILLAVCSKAIIFGYNIGIDSKADNL
                     SKQHGIEIKIYRLVYDLIEDVKNTLEGMMKPQEVEVMVGQALIKQVFKIGKNQAAAGC
                     LVVDGKVVRDAKVKIVRNGKFVFEGYLSSLKRFKNSVKEVATNTECGIGINGFSEFQV
                     GDIIQVYQKMPEGAKV"
BASE COUNT         1903 a         1636 c         1092 g         2174 t
ORIGIN      
        1 catccagtac tgttcagtcg ttcttcatcg tcttttatga tctgatacgc ttcctgagaa
       61 gttttgctca tccttaaata attccggaat actttgtcct tcaaaagtct ggctatcttg
      121 gccaatgcct taagatgtat tcctgttgcc cctgatggag cgagaacaag aaatatgata
      181 taaactgatt cgccatcaag ggaatcaaaa tcaacacctt tatctgaaat acccagagcg
      241 caaacaagat tatttacctt gtctgttttt gcgtgaggta ccgcgatccc ctgtccgata
      301 ccagttgaac caaacttttc ccgttccata agagcttcca gaacctgctt cctttcctgc
      361 aatagcccat tgttttccag aacctgcaac aattcatcga gtgcttcctg cttctgggtc
      421 gccttcaatc caatcaaaat ttctttttca ctgataaatt cactaatttt catgtttgtt
      481 cctcctctgt ttcattatta actgtagaac tgaaaaactc aatttctcca gattttctgt
      541 ttttcttatt atgtttctta tcgtgcaatt tgctcaggtg atttttcagt ttgtttataa
      601 ggtcatccag tccagcattc aaattgttcg aactggtttt cataaatatc acacgactgc
      661 gataccgtat cttcgcctca aaaataaatt caaatttttc ctttttaaca atgagatgta
      721 cctctggttc tccagagaca aatcgttcaa gacgggaaaa cttttcttct gccagttgtt
      781 caaaaccaga atccagatgt acgtgtactg gctgcagatg aaaaatcata tcaacctcct
      841 gtattatggt ttttcaataa ccacactact gctcacattt gtctggttta aagaaacaag
      901 tacaagccca gggaaatagt gtctgagaat ttctacgtaa ttcttaccat cttctgccat
      961 tcttctcgca ccccactgac acattccaac gccgtgccca tatcctttac cactaaaaac
     1021 aaaattatta ccattttttg aaacagtaaa cattgtactt ggcaaagttc ttgcggattt
     1081 aaatgttgta ttactgttta ttgttaacat tctaaaattt tctgcgctaa ttactttttt
     1141 cccgaatttt cctgaaaatt caattcgttt aatacgcccg ctgctatcga atccagcaag
     1201 attgattgtg ttaacatctg ttccaacaat agaactgaca tcatccctgg tataggtttt
     1261 gctccacgca taaaatggag taccctcaca atatgggtct tctacaccat taagcaaaag
     1321 gtacttccct ccccagatat cctcagcgga agttgtgtgc cccccacagc acgcatgaaa
     1381 aaaagtcgga ataatattat atcctttttc ttctccctgt ccaacaagca cctgactgga
     1441 agtttcttca acggctctga caatagcttc gttttcactt attacaccac catatacctg
     1501 gtgatgaatt gtatcactta catcataagg aatgtttttt ttcatcgcaa ggtctttaaa
     1561 gtgatatatc agggttcttg aacaaactgc ctgggctttt aatgcctcaa atggccatga
     1621 tttttccatc tcttttccga caacaccaaa caaatattct tcaacatcca tcgtattgat
     1681 cacaaggtac tttttgttcc atttgaccag ggacaactgg cccctgtatt ttcccctgaa
     1741 agatgacaga actggtctct gactaccttc ccagatacaa attctgacag tttcagcgca
     1801 gcataatgtc agatttaaca aaaagatgct aattgctgcc agcagtttct tttttgaaac
     1861 gaacctggac atttccttca aacctgagtt tgttttcttt gaaagtgtat gtgatgcggt
     1921 cggcactgta aaggtttgaa ccttgtatta cctgtggaga tccttccata actacggtat
     1981 catccttttc aatatatgtc agtttttcac acgttgctga aaaattggtt tgagagtttt
     2041 ctggattttt ctgggttatc ataacattgt tctgtatcac gattttcata acagcctggg
     2101 ttgatggatc aagaaacaat tctcctgttc ctttggaacc agtcctaatc tcaagaataa
     2161 aatttttact cttgatttca atcgcaggtt gtgaaactgc tgtatgcgag tcctgctttt
     2221 tttccgctgc aagtccttcc agggtaattt tgaacgaact gtcgaaaaat tccagtttga
     2281 aattcttcaa aatttgtccc gtttcctgag tttccagagt tggtttcttg cgagtgcacc
     2341 ctgaaatcat cagtaaaacg acggctaaaa ttccaacgat ttttccagca ctttttgcca
     2401 ttttccctgt cctttcaata tcacttcaac gcattctcta aaaacccctt taccggcatt
     2461 tacggatgtc acatacatta caacattttt cacttcctcg tctgcatcac gtggagcaac
     2521 ggaaaaacca acgtttctca acacatcaat atcaagtata tcatcgccaa tataacagac
     2581 ctcttccatc gccaaacatt ctttctcaca tatttttctc atcaccgcca ttttattgtc
     2641 aacattttca tagatagaat ctaccggcac atcagaaaat ctgtttttta gaatacccgt
     2701 tttctttcct gaaataatta cacttctgag ccctgccagt ttcaatagtt taaaagcaaa
     2761 cccatctctg actgaaaaaa atttcccctg attcgcaaca ccgctataaa atatcccccc
     2821 acttgttaac actccgtcaa catccgtagc aacaagttta attctgcaaa catccatatt
     2881 aaaactcctg actgaaatgc ctcttcccca aaagatcatg aagatgtaca atccccttcg
     2941 ctttaccaat tttatcccgt atcacaagac aggtgatttc tttctcttcc ataatggcaa
     3001 gcgcctgaac cgcaagtttt tcctcttcaa tacccagggg atttggagtc atgcattcca
     3061 ataccttgca actgaaaaca ttcttctttt ttgccagcaa tcttctgaga tctccatcgg
     3121 taataatgcc aataaccctt gattttctat cagtgacaag tgtgaaacca aggttttttc
     3181 tgttaatttc tattattgca tcttccattg tcctttctga atctacaaca ggaattttct
     3241 ttcctgtttg catcacatcc ttcaccttca acatcaacct ccgaccaaga ttgcctccgg
     3301 ggtgataaaa ggcatagtcc tgtctctgaa atcctttgac tgacagcaga gttattgcca
     3361 tggcatctcc aagaacaagt gcagtggtcg cgctggaact cggtataaga gaaaacggat
     3421 ctgcctcctg aatggttcca gtattcaaaa caatggtact ctgcaaacca agagaacttc
     3481 tggcatcaga agtaatactg ataatgggag tgccaatttt ttttaacgaa ggcactattt
     3541 tcaaaatttc atccgtctca ccactatttg aaatggcaac aactacatcg gacccacaaa
     3601 ccattccaag gtccccatga atcgcctctc caggatgcag aaacgttgat ggtgtgcctg
     3661 tacttgccag cgttgcggaa attttcctgc caatgatccc gcttttccca atgcctgtca
     3721 caataacctt tcctttgcac gaagcaagca tcctgatggc tttcacaaat tctgaaccga
     3781 gatgcttctt tgctctgaga agattggaaa cctcaatatc tattaccctt tttgccgctg
     3841 tcaaaatttt tctttcactg aagtttgtct tcatattttt ccctgatttt tatcacagta
     3901 ttccagattt ttcccagttc atccagcaga tatttcattg aagaaagagc caccatatta
     3961 ggaccatctg acagcgcttt ggctggttca ggatgaacct caagaaaaat agcatcacat
     4021 ccaaccgcaa cagcagccct gcacagatat ggaacaaatt ccttctgacc acctgatgat
     4081 ttgcccatgg ctcctggctg ttgaacactg tgtgtaccgt caaaaacaac aggccatcca
     4141 taatttctca ttacaggcaa cgaacgaaag tccaccacca gattattata cccaaaactg
     4201 gtgcctcgtt ccgtaataag aattttttta caaccggaag atgtaagttt ttctacgatg
     4261 ttaccagttt cccagggcga cataaactga ccctttttca cattaacagg tttccctgat
     4321 tttgccgcac taacaagaag atctgtctgc ctgcataaaa aagcaggaat ttgaataaca
     4381 tcaacaactt ctgaaaccgg ttttacctgg gtaacgcagt gaacatcagt aagaacagga
     4441 acaccaattg tgttttttat agattcaagt atccgaagtc cttgttcaag accaggtccc
     4501 ctgtaagaat ggattgaaga ccgattcgct ttatcaaagg accctttgaa aatataagac
     4561 atgttgaatt tctgtgtcag agagtgcatg gattctgcaa ccttcaatac aagagtttca
     4621 ttttccagca cacatggccc ggcgatgata catagtttag aacccccgac aacgcaatct
     4681 ctgatacgta caatattcat aactggctgg tatcaacctg gtggccattc cattattcga
     4741 ccaccaagga catgaatatg aagatggtca acactctgac cggcatttct gtttgtattt
     4801 atcacaattc taaaacctgt ctctgctatt ttcgtttgtt ttgcaatttc tgtcgcaaca
     4861 aagagcatct ttgccagaat tgtggaatct tgttcgttga cattcagcaa atttgtaaaa
     4921 tgcctgcgag gaataatcag taaatgcacg ggtgcctgag gattaatatc atgaaacgca
     4981 ataatatctt cactttcata tactaatctt gcaggatttt taccttcgga tattctgcag
     5041 aatacacaat cctgattcac acttttgctc cttcaggcat tttttgataa acctgaatta
     5101 tgtcgcccac ctgaaattca ctgaaaccat taattcctat accacactca gtattggtcg
     5161 caacttcttt tacagaattc ttgaatcttt tcaaactgga aagatatcct tcaaaaacaa
     5221 attttccgtt cctgacgatt tttaccttcg cgtccctgac aaccttacca tcgaccacaa
     5281 gacaaccagc agctgcctga tttttaccga ttttgaaaac ctgttttata agcgcctgtc
     5341 ccaccattac ttcaacctcc tgcggtttca tcataccttc aagcgtattt ttgacatcct
     5401 ctatcaggtc atatacaagt ctataaattt taatttctat tccatgctgt ttggaaaggt
     5461 tgtcagcttt tgaatcaata ccaatgttat aaccaaatat aattgccttt gaacatactg
     5521 caagaagaat gtccgattca ctgattgaac caagaccaca atgcaaaagg ttaacagatg
     5581 gaccctgttt ttccacggat gactgactga gtttttcaag agatgatttt atcgcttcct
     5641 gagaaccaat tgaatctgtt ttaagaataa tctttaattc cttaagtgtc ccctgaataa
     5701 tctgcttctg aaggtcttca agtgtaagaa ctttttgcac cttttcccct gtggatttgt
     5761 ttgcctgttt tgtttcttca gcaattctgc gtgcatctgc ctcagaatca acaaccttga
     5821 aaagatctcc tggaactgga gtggaacttg cgccaagaag ttccacagga gttgatggtc
     5881 ctgctttttc aattcttttt ccattttcgt caaacattgc tctaaccttg ccccaggatt
     5941 caccgcagac aaaaaactcc cctgtacaca ctgacccatt ttgaacaagc aggtcaataa
     6001 caggtccttt ttgtttgtga agatacgctt caagaacaac accctcacca ggtctttcag
     6061 gatttgcctt caattccatc atttcaccca taagaagaat aatttcaagc aattccttaa
     6121 tgttggctcc tgtcacgcca gaaacattaa gaaaaaccgt ctgtcctccc cattcctcag
     6181 gcacaagccc atactcactc agttttttct tcactatatc aggatttgca tttggcctgt
     6241 caatcttatt gatagcaaca atcacaggta ctcctgctgc cttacaatga tttatggctt
     6301 ccactgtctg caatttcaca ccctcatcag cagcaacaac aagcaccaca atatctgtaa
     6361 tctgtgctcc acgggatctc attgccgtga aaatttcatg tccgggagta tctataaaaa
     6421 caatggaacc ttctttcaga ttcaccttgt aagcaccaat cctctgagta atttgaccga
     6481 attctttcgc agcaacattt gatttacgaa tgtaatcaag tatcgttgtt tttccatgat
     6541 caacatgtcc cattactgtc accacagggg cgcgtggaac aaggtcgtta tcggtttcta
     6601 cggcagtacc cagaatgtag ctttctcgtg ttttcttaaa ctgcacggag acaccgataa
     6661 tatcgcacag tttttgtatg ttttccggtc ccaatctatc gttaattgcc gcgctgatac
     6721 caatgtccga aagatttttg ataatttctg ctgctgaaat acctaatttc gaagcaattt
     6781 ctccaacagt ttctgtgccg gttga
//
LOCUS       DAORYU010000060         6797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_7840_length_6797_cov_4.898843, whole genome shotgun sequence.
ACCESSION   DAORYU010000060 DAORYU010000000
VERSION     DAORYU010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6797
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7840_length_6797_cov_4.898843"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="PLW07_03035"
     CDS             <1..654
                     /locus_tag="PLW07_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOL49529.1"
                     /translation="VTFHCDGVQVAGKIPVSVKKWNVDFFALSAHKFYGPKGIGALYV
                     KKGVKFRPITYGGHQEKGLRPGTENVPGIIGLGKACEIAENLMAQDASKEKELRDLLE
                     KKLVENIPEILVNGHPEKRLYNTLNICVKYVEGESMLLHLDMEGICASSGSACTSGSL
                     EPSHVLLALGIPHEIAHGSLRFSLGRHNTLSDIEKTAEVLPKIVEKLRKMSPFWDKK"
     gene            651..1562
                     /gene="nadA"
                     /locus_tag="PLW07_03040"
     CDS             651..1562
                     /gene="nadA"
                     /locus_tag="PLW07_03040"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014042142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOL49530.1"
                     /translation="MNEILIDEIKKLQKEKNAVILAHNYQLPEVQDIADFVGDSLELA
                     KKAKEVKTASMIVFCGVQFMAETAKILNPEKKVLLPAQDAGCPLADMAKAEDVIEMKK
                     KYPDAFVVSYVNTSAQVKAVSDVCCTSSNAPSVVKNVPSDLIIFLPDKNLGYYVQKQV
                     PEKKLIVWDGYCFVHRQFTLDDLKKAREKYPDAEIIVHPECNPEIQENADFVLSTSGM
                     LKRVGISSAKTFIIGTEEGILHRMKKQNPEKQFFSLGSARICFNMKKTTLEKVKDCLV
                     NERGEIILDQDIMIKARNALEGMIKYV"
     gene            1937..2329
                     /locus_tag="PLW07_03045"
     CDS             1937..2329
                     /locus_tag="PLW07_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HOL49531.1"
                     /translation="MYSEKLMDHFKNPRNMGRIENPDGIGRVGNPVCGDVMWIYIKVG
                     NNENGEEFIEDIKFETFGCGAAIATSSVITELAKGKTLKEAEKITNQNVIDTLEGLPP
                     LKKHCSLLAEEGLQAAINDYRERKKAQI"
     gene            2362..2586
                     /locus_tag="PLW07_03050"
     CDS             2362..2586
                     /locus_tag="PLW07_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOL49532.1"
                     /translation="MKAKVEEALKEIRTFLQTEGGDCELVDVSDGIAKVKLTGSCAGC
                     PFAQITLTMRVEKIIKERVPEIKEVVNVAE"
     gene            2573..3394
                     /gene="larE"
                     /locus_tag="PLW07_03055"
     CDS             2573..3394
                     /gene="larE"
                     /locus_tag="PLW07_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HOL49533.1"
                     /translation="MLRNEIEKKKRKLEKILQKAGKILIAYSGGVDSTFLLKVAVDCL
                     GKDNVAAFIEKSEFDAPSEINFAKKFATSLGVKVFCLKLNKLTDKQLSNNPPDRCFYC
                     KKELFSQMADIARKYDYNAIADGSNVDDKFDYRPGNKAKLLFQVISPLQMANLTKADI
                     RMLSKEMGLPTWNKPQMACLASRIPYGEKITEERLKRILKAEELLGKLGFHIVRVRDY
                     GDLCRIEVSDDDFEKIIQFRREITKQLKEFGYIYITVDLEGYHTGSMNRTLPSKK"
     gene            3396..4616
                     /locus_tag="PLW07_03060"
     CDS             3396..4616
                     /locus_tag="PLW07_03060"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOL49534.1"
                     /translation="METLTGIIKEIWNLFMAMSVYLVFGFLVAGIVSVFLKPEKIFRH
                     FGNSGFSSILKAVLFGVPLPLCSCGVLPPAAGLYKSGASKGSTLAFLISTPTTGIDSI
                     IATYGLMGGIFMILRILASVVIGIVAGIVSDIFSGEKDTAFVKQVPEISCSSSGAWIK
                     EALRFGFVEVYQSTARWIFLGVIIGGLISYLVPENILSMYAGNRWMTYLIMLVVGLPL
                     YVCATGSIPIAASLAAKGISPGACLVFLIAGPATNTVTMLFVGKTLGKKHLIIYLVSI
                     VLGALIFGLLLDNILINYNLSIQPFHHSHNKLFEVLSLVCSFVLIGISIVSVFTHMLS
                     TRKKGSFCFVYNVPDISCQNCVRNIKNVLLTESGIKSVKVDVKSKKVTICADFNDDKK
                     LKQLLDQAGYPASE"
     gene            complement(4618..5685)
                     /locus_tag="PLW07_03065"
     CDS             complement(4618..5685)
                     /locus_tag="PLW07_03065"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOL49535.1"
                     /translation="MNIEHPSKKEYEKIMQFLEESYDHSRGFFYVNYPQVMSKDTMDC
                     DNIFLIREKNKIASLVSIFPQKVYLHGRQVNIGGIGSVSTHHDCRNRGYMKILMDFCI
                     DEMKKRNYAFSILGGDRQRYNFWGYEICGTNAVITFSQRSFEKSGTTQTIPTFRYDGS
                     KEILQKIKDVHEQMPCYVERSMKWIEKLFNERIHTQLYYTANKNGFAYVVAIGERIER
                     KIVEIGGDKTLWCPLIYSLLKRWNNNAVDVLFPGNIPEFSVLLKNAAWFRIEPFSMIK
                     ILSFTDTMKFLFGKDFHYGCMLKMSETKEIIGDTGNKKIIEFDEKSWSRILFGPSFIE
                     TPDCLRKITPAQFYWWYLDHI"
     gene            complement(5701..6753)
                     /locus_tag="PLW07_03070"
     CDS             complement(5701..6753)
                     /locus_tag="PLW07_03070"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOL49536.1"
                     /translation="MNDWGFEPDFENLKNTLLWKKNYRVPNMEFVIDREIKEAYLKKE
                     VTMLEDEIEFRHRAGYDYIWISKGMIDPAGTVNKEFISDDPSKHFKGKDTRVWAEEHT
                     GIIKTKEDIENYPWPDIDNMTYSEFEKAGKLLPEGMKIFGVCGKIFVATWMLFGFENF
                     VIATIEQPKLVDELINIIGEIQVKSCRRMLEFDCVGGIWVPDDIAYRTGTMMPPEWLK
                     EKIFVFYKKMAGMIKEAGKLFLYHSDGNLLKMLDIIIETGFNALHPIEPESMDISHVR
                     KLVDKKLALIGNVCVHTLATGTKQDVINLVMERIEKYGKEGGYALGSSNSIPNYVPIE
                     NYLTMLETNAKYGVIS"
BASE COUNT         2126 a         1184 c         1354 g         2133 t
ORIGIN      
        1 gtgactttcc attgcgatgg cgtacaggtt gctggaaaaa ttccggtatc agtaaaaaaa
       61 tggaatgttg acttttttgc gctttctgca cataagtttt atgggcccaa agggattggt
      121 gctctttatg taaagaaagg agtaaaattc agacccatta catacggggg acaccaggaa
      181 aagggtttga gacccggtac agaaaatgtt cctggaatta tcgggctcgg gaaggcgtgt
      241 gaaattgctg aaaatttgat ggcacaggac gcatcaaaag aaaaagaact cagggacctt
      301 cttgagaaaa aacttgttga aaatattcct gaaattctcg tgaatggaca ccctgaaaag
      361 cgtctctaca atacattgaa tatttgtgtg aagtatgtgg aaggagaaag tatgctatta
      421 catcttgata tggaagggat ttgcgcatct tctggttctg catgtacttc tggttctctt
      481 gaaccatcgc atgtattgct tgcactcggt attcctcacg agattgccca tggttcgttg
      541 agattcagcc ttggaaggca taatacactt tctgatattg aaaagaccgc agaagtactt
      601 ccgaaaattg ttgaaaaatt aagaaaaatg tcgcctttct gggataaaaa atgaatgaaa
      661 tattgataga tgaaatcaaa aaattgcaga aagaaaaaaa tgctgtaatt ctggctcata
      721 attatcagtt accagaagta caggatattg ctgattttgt tggagatagt ttagaactgg
      781 caaaaaaggc aaaagaagta aaaacagcca gtatgattgt tttttgtggg gttcagttta
      841 tggcagagac tgcaaaaatt ttaaatcctg aaaaaaaggt attgcttcct gcgcaggatg
      901 cagggtgtcc ccttgctgat atggcgaaag cagaagatgt gattgagatg aaaaaaaaat
      961 atcctgatgc atttgttgtt tcttatgtga atacatctgc gcaggtaaag gcagtgtctg
     1021 atgtttgctg cacatcttca aatgcgcctt cggttgtaaa aaatgtgcca tcggatttga
     1081 taatattttt gcctgataaa aacctgggat actatgttca aaaacaggtt cctgaaaaaa
     1141 agttgattgt gtgggatggt tattgctttg ttcacaggca gtttaccctt gatgatttaa
     1201 aaaaagcaag ggagaaatat cctgatgctg aaatcattgt acatcctgaa tgcaatcctg
     1261 aaatacagga aaacgctgat tttgtattat caacatcagg aatgctgaaa agagtaggca
     1321 tatcttcagc aaagaccttt attataggaa cagaagaagg aattcttcat aggatgaaaa
     1381 aacaaaatcc agaaaaacaa ttcttttccc ttggatccgc aaggatatgt ttcaatatga
     1441 aaaaaacaac actggaaaaa gtgaaagatt gtcttgtaaa tgaaaggggt gaaattattc
     1501 ttgaccagga catcatgatt aaagcgcgaa atgcgctgga ggggatgatt aaatatgtgt
     1561 agggtgggaa tgggaaagaa aatgggatgt ctttattcac ttgggtgctg cggcacagac
     1621 aaaactacgg ataaatccgc gtcccctgca aacttgcctt ctccgtatac attctcatac
     1681 tgcgaaggct tcggacaagc aggggtaagg atattgcgca gcgcaggagc ttttatcctt
     1741 gttttgttac tgtgccttcg caaatcgttc ataaagacat ccattttctt tcctggagag
     1801 aaagtggaga aaaaaccgaa gaaacgtgat acgggatatc tttattcacc tgggtgctac
     1861 ggcacagaca aaactacgga taaatccgca tcccctgcaa tgtgtcattg tataacctga
     1921 cgtaaggaga aaacaaatgt acagtgaaaa attgatggac cactttaaaa atccaagaaa
     1981 tatggggaga attgaaaatc ctgatggtat tggtagggtg ggtaatcctg tgtgtggaga
     2041 tgtgatgtgg atatacatca aagtgggtaa caatgaaaat ggtgaagagt ttattgagga
     2101 tataaagttc gagacatttg gctgtggcgc tgcaattgcc accagttctg tcattacaga
     2161 actggcaaaa gggaaaactt taaaagaggc agaaaaaatt acaaatcaaa atgttattga
     2221 tacgcttgaa gggcttccac cactgaaaaa acactgctcc cttcttgctg aagagggttt
     2281 gcaggcagcg attaacgatt atagagaaag aaaaaaagca caaatttaac cggggaggca
     2341 ataatggctt caaatgagca gttgaaggcg aaagtggaag aagcattgaa agaaataagg
     2401 acctttcttc agacagaggg aggggattgc gaacttgttg atgtttcaga tggcattgcg
     2461 aaggtgaaat taactggaag ttgtgcggga tgtccttttg cccagattac tttaacgatg
     2521 agggttgaaa aaataatcaa ggagagggtt cctgagatta aggaagtcgt aaatgttgcg
     2581 gaatgagatt gagaaaaaaa agagaaaact tgaaaaaatt ttgcagaaag cagggaaaat
     2641 cctgattgct tattcaggtg gcgttgatag cacatttctt ttaaaagttg ctgttgattg
     2701 cctcgggaaa gacaatgtgg ctgcgttcat tgaaaagagt gaatttgatg ctccttctga
     2761 aatcaatttt gcaaagaaat ttgcaacatc ccttggagta aaggtatttt gcttaaaatt
     2821 aaataaactc actgataaac aattatcaaa taaccctcca gatagatgtt tttactgcaa
     2881 aaaggaactg ttttctcaaa tggcggatat tgccagaaaa tacgattata atgcaatcgc
     2941 tgatggttcc aatgttgatg ataaatttga ttacagacca ggtaataaag caaaattgct
     3001 atttcaggta atttcacctt tgcagatggc aaacctgaca aaggcagaca tcagaatgct
     3061 ttcaaaggaa atgggtttac caacatggaa taagccacag atggcatgcc ttgcttcaag
     3121 aataccgtat ggtgaaaaaa ttactgaaga aagattaaaa agaatactga aagcagaaga
     3181 actgcttggc aaacttggtt ttcacattgt cagggtcagg gattatggag atttatgtag
     3241 aattgaagtg tctgatgatg actttgaaaa aattattcaa ttcaggagag aaattacaaa
     3301 acagttgaaa gaatttggtt acatttacat tactgttgac cttgaaggat atcacaccgg
     3361 cagtatgaat agaaccctgc catcaaaaaa ataaagtgga aacactcaca ggaattatta
     3421 aagaaatctg gaatcttttt atggcgatga gtgtgtacct tgtttttggg tttctggtgg
     3481 ctggcattgt tagtgttttt ctcaagccag aaaaaatttt caggcatttt ggcaacagcg
     3541 gtttttcttc cattctgaag gcagtgttat tcggagtacc actgcctttg tgctcttgtg
     3601 gagtacttcc acctgcagca ggtctatata aatcaggagc aagcaagggt tcaacacttg
     3661 cttttttaat ttccactcca acaacaggta ttgattcaat aattgcaaca tatggtttga
     3721 tgggtggaat atttatgatt cttagaattc tggcatcagt tgttataggg attgtggctg
     3781 gtattgtgtc tgatattttt tcaggggaaa aagatactgc ttttgtaaaa caagtaccag
     3841 aaatcagttg ttcttcatct ggagcatgga tcaaagaagc attaagattt ggattcgtgg
     3901 aagtttatca atctactgcc agatggattt ttcttggagt tatcataggt ggattaattt
     3961 cctaccttgt tccagaaaac attttatcaa tgtatgctgg taatcgctgg atgacatact
     4021 taatcatgct cgttgtcggg cttccgctat atgtgtgtgc gactggttcc ataccgattg
     4081 cagcgtctct tgcagcaaaa ggaatcagtc ctggtgcatg tcttgttttt cttatagcag
     4141 gaccagcgac caatactgtg acaatgctgt ttgtgggaaa aaccctcggg aaaaaacacc
     4201 ttataatata ccttgtttcc atagttctcg gggcattgat attcgggtta ctgcttgata
     4261 acattttgat taattataat ctttcaattc agcccttcca tcacagccac aacaaacttt
     4321 ttgaggtcct ttctcttgtt tgttcatttg ttcttattgg catatctatt gtgtctgttt
     4381 ttactcatat gttgtcaacc agaaaaaagg gtagtttctg ctttgtttat aatgtgcctg
     4441 acatttcctg tcaaaattgc gtgagaaata ttaaaaatgt gcttctaaca gaaagcggta
     4501 tcaaatcagt aaaggtggat gtcaaaagta aaaaagttac aatatgcgca gatttcaatg
     4561 atgataagaa actcaagcaa cttcttgatc aggcaggtta tcctgcttct gaataaatta
     4621 gatgtggtca agataccacc agtaaaattg cgctggcgtt attttcctca aacaatcagg
     4681 ggtttctata aacgatggtc cgaatagaat tcttgaccaa cttttttcat caaattcaat
     4741 aattttttta tttcctgtat cgccaatgat ttcctttgtt tcagacattt ttaacataca
     4801 accataatga aaatccttcc cgaaaagaaa tttcatggta tctgtaaatg acagaatttt
     4861 tatcatagaa aatggttcaa tcctgaacca tgctgcattt ttgagcagta cgctgaattc
     4921 tggaatattt ccaggaaata atacatccac agcattattg ttccaccttt taagcaggga
     4981 ataaatcagt ggacaccaca gtgttttatc tccgccaatt tcaacaattt tcctttctat
     5041 tctttctcca atcgcaacaa catacgcaaa tccattttta tttgctgtgt aataaagttg
     5101 agtgtgtatc cgttcattaa ataatttctc aatccatttc atgcttcttt ctacatagca
     5161 gggcatttgt tcatgtacat ctttgatttt ttgaagaatt tcctttgaac catcgtatct
     5221 gaaagtggga atagtttgtg tggttcctga tttttcgaat gacctctggg aaaaagtaat
     5281 cacggcattt gtaccgcaga tctcatatcc ccaaaagttg tatcgctgtc tgtctccacc
     5341 aagaattgag aatgcataat ttcttttttt catctcatca atacagaaat ccatcaaaat
     5401 tttcatgtag cccctgttcc tacaatcatg atgagtggaa acactgccga tgcctccaat
     5461 atttacctgc cttccatgta aataaacctt ctgggggaaa atgctaacaa gcgatgcaat
     5521 cttatttttt tctctgatga ggaaaatatt atcacagtcc atggtatctt ttgacattac
     5581 ctgaggataa ttaacataga aaaatcctct tgaatggtca taggattctt caagaaattg
     5641 cataattttt tcgtattctt tttttgatgg atgttctatg ttcatgtttt ctccctgtgt
     5701 ttatgaaata acaccatatt ttgcatttgt ttcaagcatt gtcagataat tttcaatcgg
     5761 cacatagttt ggtattgaat tgcttgaacc aagggcatat ccaccttctt ttccatattt
     5821 ttcaattctt tccatgacaa gattgataac gtcctgtttt gtgcctgtgg caagagtatg
     5881 aacgcacaca tttccaatta aagcaagttt tttgtctaca agttttctta catgtgaaat
     5941 atccattgat tctggttcaa taggatgaag cgcattaaat ccagtttcaa tgatgatatc
     6001 cagcatcttc aaaaggtttc catcagaatg atagaggaac agttttcctg cttcttttat
     6061 cattcctgcc atctttttgt aaaagacaaa aattttttct ttcaaccact ctggaggcat
     6121 cattgtaccg gttctataag caatatcatc tggaacccat attccaccaa cacaatcaaa
     6181 ctcaagcatc cttctacacg attttacctg tatttctcca ataatattaa tcaattcatc
     6241 aacaagtttc ggttgttcaa tggtagcaat aacaaagttt tcaaagccaa aaagcatcca
     6301 tgtggcaaca aaaatttttc cacatacacc gaatattttc attccttctg gtaataattt
     6361 tcctgccttt tcaaattcag aataggtcat gttatcaatg tcaggccagg ggtaattttc
     6421 aatgtcttcc ttcgttttaa taattcctgt atgttcttct gcccagacac gagtatcctt
     6481 gcccttaaaa tgttttgaag ggtcatcaga aatgaactct ttattgactg ttccagcagg
     6541 gtcaatcatt cctttactga tccagatgta gtcataacca gccctgtgcc tgaattcaat
     6601 ttcgtcttca agcattgtta cttccttttt taagtatgct tccttaattt ctctatcaat
     6661 gacaaattcc atattcggca cacgataatt tttcttccac aaaagagtat tttttaaatt
     6721 ttcaaaatcc ggctcaaatc cccagtcatt catttcttcc ccgtttttaa ttcaacaatt
     6781 gtatatggac catctgg
//
LOCUS       DAORYU010000061         6780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_7862_length_6780_cov_4.286097, whole genome shotgun sequence.
ACCESSION   DAORYU010000061 DAORYU010000000
VERSION     DAORYU010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6780
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7862_length_6780_cov_4.286097"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="PLW07_03075"
     CDS             <1..422
                     /locus_tag="PLW07_03075"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HOL49537.1"
                     /translation="WPARFQVLSENPFVILDGAHNPAGILSLKQALKQYFPESKPIFL
                     VGILKDKNWDTMVKILSKIGKKFIFTQVQTTRSLPPSTLFSKMKEIMPEADVEIIENP
                     SQALEKLLAMKGNEPRVICGSLYLAGDVLRTVKTKLL"
     gene            complement(417..863)
                     /locus_tag="PLW07_03080"
     CDS             complement(417..863)
                     /locus_tag="PLW07_03080"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOL49538.1"
                     /translation="MKDELVVPPILENLSIVAEFLQEKIKETHLTSRKAWELLLVTDE
                     ICSHIIHFRKYDSTMGKMRILWEGLPDKVVVTIETSGIPFNPLELNSMDNVKEEEEES
                     LGGMGIHLVKQMIDEIDYKRTNNSNILKITKLKRSRRNRINNKNNL"
     gene            complement(853..2046)
                     /locus_tag="PLW07_03085"
     CDS             complement(853..2046)
                     /locus_tag="PLW07_03085"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOL49539.1"
                     /translation="MKIAHVITRMIIGGAQENTLATVSGLKQRGHEVILISGPSRGPE
                     GSMQKDIEKTDVYLIIIPELVRDISIWHDVIAFFKLGQIFRKHRFDIIHTHSAKAGFI
                     GRIAAKFFSKKSKVVHTLHGSSFHPYQNALVRYFYILCEKIASKFTDYFISVSSVILD
                     NHIKRGIVRKQGHSVIRSGFQVEEYKQAYQFREEVRKQLGLINKNILIGMIGRLFPLK
                     GQEYLLRVFAKISDDYPDTHLMLVGDGILRKDFENFVKANHLQQRVHFTGLVEPEKIP
                     LFASSIDIGVHTSLREGLPRAVVQILAAGSPVVAFNIDGAKEVIQNGINGFLVPARNE
                     AMLEEKLRLLIEHPEERKRMGIAGQHMVFEEFSTEKMVAKIEKVYFNIIEKNSSHYRK
                     EPDEG"
     gene            2246..2449
                     /locus_tag="PLW07_03090"
     CDS             2246..2449
                     /locus_tag="PLW07_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49540.1"
                     /translation="MTEEGIFCSDECAQTAKIHSERMKQIEQQRDSYRGARWTGIAKL
                     IKIIVFLVILFVIYKFVIVRFIK"
     gene            complement(2442..3233)
                     /locus_tag="PLW07_03095"
     CDS             complement(2442..3233)
                     /locus_tag="PLW07_03095"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOL49541.1"
                     /translation="MIEKPITFQSGRSQIVGILHLPDRKTTYEKFPAVIFCHGFSGNK
                     SESHFIFTKTARQIASCGLACLRFDFMGSGDSAGSFEEMTLETEIFDAFQAYEYLAGL
                     PFINKENIGILGLSMGAIPAVIVASKIPLKSLCLWAPVAYPEEISKKILTAAVRKKLQ
                     QQGTAYLNGAGLRIGKHFINSLKKINPVEAASRFCGHTLIIHSKDDPTINVSHALAYY
                     KAFHNAAITKRMIILEKGGHTFVTEQSENIVIQETASSFTQTLIT"
     gene            3323..4513
                     /locus_tag="PLW07_03100"
     CDS             3323..4513
                     /locus_tag="PLW07_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49542.1"
                     /translation="MKIFWQSEACDLKHDPVSGALITRLTCSVMNNINIYCEQPYTCP
                     DGKRIAYTRSFGPDPRIPPYQLCVADIEKLKVALIEPEVSGLLVGTSAWSGWIYYLRP
                     NRELMRVNIVTLEKEIIMTRCDLPEGMCFDSVSPDHRYLVGVSRNRGGDSEIIRLDMK
                     EKTWKVIFRHVDILSHVQFDFVGGRDILVQHNRTQRLGSDGLRIESRESTLEATTHFI
                     IDIDGLNLRQLKVGPPYTAGSTGHSGWIAHTGKIGLTVRWPAMGEEEPSARFKQVHDS
                     RHRQGNFVIVGPDDEKPFIFSAPEHLFNHVNISKCGTYFVCDSYKNAIPGPIEIVVGN
                     IKTGKYRTLVSNCGAQGGGAAASHPHPYMTADNKNVIFNADPFGICHVFAARIPEDFL
                     KSLE"
     gene            complement(4546..4980)
                     /locus_tag="PLW07_03105"
     CDS             complement(4546..4980)
                     /locus_tag="PLW07_03105"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOL49543.1"
                     /translation="MAKLVKWEPFRELDEIRTGFDRLLDRFKTDFYGDHIWSPAVDIT
                     DTGDSIVVKAEVPGFDKKNISISLAGDTITISGKTQEEKEEKKAHYLYKERVSGSFTR
                     SFTLPVPVDRNKVKATCKDGVLEIVLPKAEEAKAKEIKIDVE"
     gene            5116..5463
                     /locus_tag="PLW07_03110"
     CDS             5116..5463
                     /locus_tag="PLW07_03110"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOL49544.1"
                     /translation="MADEKIQYLEKIYKAVANRNRLRIIKLLENGSLCVCDIKREIGT
                     SQPSVSRHLSILKKAGLVCGWKKGEWVYYRIQKSSDDLITQILQQIFNTLKHEKITGI
                     RIKKNRCVNKHKK"
     gene            5473..6525
                     /gene="arsB"
                     /locus_tag="PLW07_03115"
     CDS             5473..6525
                     /gene="arsB"
                     /locus_tag="PLW07_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HOL49545.1"
                     /translation="MEQKKLSLLDRLLTLWIFLAMAVGVLSGYLFPGIVDFWNQFQVG
                     TTNIPIAIGLILMMYPPLAKVKYEEMGKIFSNKRLLAISLIQNWIVGPLVMFILAVLF
                     LRHYPEYMIGVVLVGLARCIAMVIVWNELADGDRELAVGLVAFNAIFQVLLYAAYIYI
                     FIVFFLGKLNLVKNLEINISMADAAKTVFIYLGIPFLAGLITRFSLLKIKGIQWYEKV
                     FVPKISPVTLVALLFTIIVMFSIKGEYIVKLPLDVIRVAIPLCLYFLIMWFATFFLLR
                     KLGVEYSKTVAVSFTAASNDFELAIAVAVAIFGIGSNQAFATVIGPLLEVPVLIMLVN
                     VALKFKKKRGNCESQL"
BASE COUNT         2004 a         1232 c         1332 g         2212 t
ORIGIN      
        1 tatggccagc aagatttcag gtgctttcag aaaacccttt tgtgatactt gatggtgcgc
       61 ataatcctgc tgggattttg tctttgaaac aggcgctcaa acagtatttc cctgaaagta
      121 aaccaatttt tcttgttggc attctcaaag acaagaattg ggacactatg gtgaaaatac
      181 tttcaaagat tggtaaaaaa tttattttta ctcaggttca aactacaaga agtttaccac
      241 cttccacgct tttttcaaaa atgaaggaaa taatgcctga agcagatgtt gaaataattg
      301 aaaacccatc acaggcatta gaaaaacttc tggcgatgaa aggtaatgaa ccaagggtca
      361 tttgcggttc tctgtatctt gcaggagatg ttttaaggac agtcaaaaca aaattactat
      421 aaattattct tgttatttat cctgttccta cgcgaccttt tcaattttgt gattttcagg
      481 atattgctat tatttgttct tttgtagtca atctcgtcaa tcatctgttt gacaagatgt
      541 attcccattc caccaaggga ttcttcttct tcctctttta cattatccat agagtttaac
      601 tcaaggggat tgaaaggtat gccagaagtt tctatggtaa caacgacctt atccggcaat
      661 ccttcccaga gaattctcat tttgcccatg gtactgtcat atttcctgaa atgaataata
      721 tgagagcata tttcatctgt cacaagcaac aattcccaag cttttcttga tgtaaggtgg
      781 gtttctttga ttttttcctg caggaattct gcaacgatgc tgagattttc aagtataggc
      841 ggtactacaa gttcatcctt catcaggttc ctttctgtaa tgcgaactat ttttttcaat
      901 tatattaaaa tacactttct caatttttgc aaccattttt tcagtggaaa attcttcaaa
      961 caccatatgc tgtccagcaa tacccattct tttcctctct tcaggatgct ctataagaag
     1021 tctcaatttt tcttcaagca tggcttcatt gcgggcaggg actaaaaatc cattgattcc
     1081 attttgaatg acctcttttg caccatcaat gttaaatgca acaacaggac ttcctgcagc
     1141 aagaatttga accactgccc ttggaagtcc ttccctcaaa cttgtatgaa caccaatgtc
     1201 aatggacgaa gcaaacaagg gaattttttc tggttctaca agacctgtaa agtgaaccct
     1261 ttgttgcaga tgatttgctt ttacaaagtt ttcaaaatct tttctcaaaa tgccatcacc
     1321 cacaagcata agatgtgtat caggatagtc atcagatatt tttgcaaaaa cccttaatag
     1381 atattcctgc ccttttaatg ggaacagtct tcctatcata ccaatgagaa tgtttttatt
     1441 gataagacca agttgttttc ttacttcttc gcggaattga taggcctgtt tgtattcttc
     1501 aacctgaaaa ccactcctta ttactgaatg accctgtttt ctcactatac cgcgcttaat
     1561 gtgattgtca agaattacac tgctgactga aataaagtaa tctgtgaatt tgcttgcgat
     1621 tttttcacac aggatataaa aatacctcac taaagcattt tgatagggat gaaaacttga
     1681 gccatgaagg gtatggacaa cttttgattt ttttgagaaa aattttgctg cgattcttcc
     1741 aataaaacct gcttttgcac tgtgggtgtg aataatgtca aacctgtgtt ttctgaatat
     1801 ctgacccagt ttaaaaaaag caatcacatc atgccagata cttatgtctc tgacaagttc
     1861 agggatgatg atgagataca cgtctgtttt ttcaatatct ttctgcatac ttccttctgg
     1921 acctcttgag ggacctgata tgaggataac ttcgtgtcct cgttgtttca gaccagatac
     1981 tgtggcaagt gtattttcct gggcgccacc aataatcatg cgtgtaataa catgtgcgat
     2041 tttcatttaa aatatattgt aactgtatta aatacaaact caaaattgtt tgaaaaagtg
     2101 tttttgacct gaactgaata aaattttata attgttaata aagacatagg agggaaaaaa
     2161 ggctgattgt tttttccaca caggtaggcc tgcagtcaca agatgtaagc aatgcggaag
     2221 gcctttgtgt tcttcctgta gaaaagtgac agaagaagga attttttgca gtgatgaatg
     2281 cgctcaaact gcgaaaattc attcagaaag aatgaaacag attgagcagc agagggattc
     2341 ttacagaggt gcacgctgga ctggtattgc aaaactcatt aagattatag tttttcttgt
     2401 aattttgttt gttatctata aatttgtgat agtaagattt attaagtaat caaggtttga
     2461 gtaaaggaac ttgctgtttc ctgaattaca atattttctg attgttctgt gacaaaagta
     2521 tgaccgcctt tttccaaaat aatcatacgt tttgtaatgg cagcattgtg aaatgctttg
     2581 taatacgcta atgcatgtga aacgttgata gtagggtcat cctttgaatg aataataagg
     2641 gtgtgtccac aaaaccttga agcggcttca acagggttta tttttttgag agaattgata
     2701 aagtgtttgc ctattctgag accagcgcca ttaagatacg ctgtgccttg ttgttgaagt
     2761 ttttttctta ctgctgcagt caggattttt tttgagattt cttcagggta tgcaacaggc
     2821 gcccacaaac ataaagattt taaagggatt tttgaagcca caatgactgc tggaattgct
     2881 cccatggaaa gaccaagaat cccaatgttt tctttattaa taaatggtaa accagcaaga
     2941 tactcatacg cctgaaatgc atcaaaaatt tctgtttcaa gagtcatctc ttcaaaacta
     3001 ccggcactgt cgcctgagcc cataaaatca aatctcaagc atgccagtcc acaggaagca
     3061 atctgtcttg cagtttttgt aaaaataaaa tgactttcag atttgttgcc tgaaaaacca
     3121 tggcaaaaaa ttacagcagg aaatttttca taagtagttt ttctatctgg cagatgtaag
     3181 atgccaacaa tttgacttct accactttga aaagtaatag gtttttcaat catttctttt
     3241 ccttgaagtt gactgcatgg tcagagttat tataatgtgt ttttagtcca tacaaaaatt
     3301 ttgctttaaa aaagggaaaa aaatgaaaat cttctggcag tcagaagcgt gtgacctgaa
     3361 acatgaccct gtaagcggag cactgattac aagattgaca tgttctgtga tgaataatat
     3421 caatatttat tgtgaacaac catacacttg cccagatggt aagagaattg cctatacccg
     3481 cagttttggg ccagacccca gaattccgcc atatcaatta tgtgtggctg acatcgaaaa
     3541 actgaaggtt gctttaatag agccagaggt atcaggtttg cttgttggaa catctgcctg
     3601 gtctggatgg atttattatt taaggcctaa cagggaactt atgcgggtca atattgtaac
     3661 gcttgagaaa gaaattatta tgacacgatg cgatttacct gaaggaatgt gttttgactc
     3721 tgtgtctcct gaccacagat atcttgttgg tgtttcaaga aatcgcgggg gtgattctga
     3781 gatcattcgg ctggatatga aagaaaaaac atggaaggtg atattcagac atgtagatat
     3841 tttgagccat gttcagtttg attttgttgg tggcagagat attcttgttc agcacaacag
     3901 aacacaacga ttgggttctg atggtctcag aattgaatcc agagagtcaa cattagaagc
     3961 cacaacacac tttattatag acattgacgg cctgaatctc aggcagttaa aggtaggtcc
     4021 cccttatact gcgggttcaa caggacattc tggctggatt gcccacacag gtaaaattgg
     4081 attaacagtg agatggcctg cgatgggtga agaagaacca tcagcgcgat tcaaacaggt
     4141 tcatgacagc cgccacaggc agggaaattt tgttattgtt ggtcctgatg atgaaaaacc
     4201 atttattttc tctgcacctg aacatctgtt caaccatgtc aatatatcaa aatgtggtac
     4261 gtattttgtt tgtgattcgt ataagaatgc tattccagga ccaattgaaa ttgtcgtcgg
     4321 gaacataaaa acaggtaagt acaggaccct tgtttcaaat tgtggtgcac agggtggcgg
     4381 cgctgctgca tctcatcctc acccctatat gactgctgac aataaaaatg tgattttcaa
     4441 tgctgatcca tttggaatct gtcatgtatt tgctgctaga ataccagagg attttttgaa
     4501 atctcttgaa tgaaaggcgg ccggattgac cggccacctt tcatattact caacgtcgat
     4561 cttgatttct tttgcctttg cttcttctgc cttcgggagc acaatctcaa ggacaccatc
     4621 tttacaggtt gcttttactt tattgcggtc cacaggtact ggcagagtga aggacctggt
     4681 gaaactgcca gacactcttt ctttataaag gtaatgtgcc tttttttctt ccttctcttc
     4741 ctgtgtcttg ccagagatgg taattgtgtc tccagcaaga gagatactga tgtttttctt
     4801 atcaaaacca gggacctctg ccttgacgac aatgctatct ccagtgtctg tgatgtcaac
     4861 cgcaggagac catatatgat caccgtaaaa atctgttttg aatctgtcaa gcaacctgtc
     4921 aaaacctgtt cttatttcat ccagctccct gaagggttcc catttaacca gttttgccat
     4981 aattaccacc tcctttttct tttttttcaa tattatagat gcattcaaac cgcacagggt
     5041 ttcaagtttt ttagattttt gacagcaaat attttcatgc taccatataa cagttttgtt
     5101 atatcctaag gacacatggc cgacgaaaaa attcagtacc ttgaaaagat ttataaggca
     5161 gttgcaaata gaaacaggtt gagaattatt aaattgcttg aaaatggttc tttgtgtgtg
     5221 tgtgatatca aaagagaaat agggacatct caaccatcag tttcacggca tttaagtatc
     5281 ctgaagaaag caggacttgt ttgtggatgg aaaaaaggag agtgggttta ttatagaata
     5341 caaaaatcat ctgatgactt gataacacaa attctgcaac aaatttttaa cacattgaaa
     5401 catgaaaaaa taacaggcat aaggattaag aagaaccgct gtgttaacaa gcataaaaaa
     5461 taggggggca atatggaaca gaaaaaactt tctctactgg accgtctttt gacgctgtgg
     5521 atttttcttg caatggctgt aggtgttttg tcagggtatt tgtttccagg tattgttgat
     5581 ttctggaatc aatttcaagt tggaacaaca aatattccta ttgcaatagg tctgatactg
     5641 atgatgtatc caccgcttgc aaaggtcaaa tacgaagaaa tgggcaaaat cttcagtaac
     5701 aaaagacttc ttgcgatttc tctgattcag aactggattg ttggtcctct ggtaatgttt
     5761 attctggcag tgttattttt aagacattat ccagagtaca tgataggcgt ggtgcttgtt
     5821 ggacttgccc gttgcattgc gatggtaatc gtatggaacg aactggcaga tggagacagg
     5881 gaactggcag tggggctggt tgccttcaat gccatttttc aggtacttct ttatgcagca
     5941 tacatttata ttttcattgt gttttttctc ggtaaactta atcttgtcaa aaaccttgaa
     6001 atcaatatct caatggcaga tgcagcaaaa actgtgttta tataccttgg tattccattc
     6061 cttgcgggtc tgatcacaag gttttctctc ttgaaaatta aggggataca gtggtatgag
     6121 aaagtttttg ttccaaaaat cagtcctgtg acgcttgttg cgcttttgtt taccattatt
     6181 gtaatgtttt caataaaggg tgaatacatt gtaaaactgc ctctggatgt tattagggtt
     6241 gcgatacctt tgtgtcttta ttttttgatt atgtggtttg ccacattttt tcttttgcga
     6301 aaacttggtg tcgaatattc aaaaactgtt gcagtgtctt ttactgctgc cagtaatgac
     6361 tttgaactgg ctattgcggt tgccgttgcg atatttggaa taggttcaaa ccaggcattt
     6421 gcaactgtta ttgggcctct tcttgaagtt cctgtgctga ttatgctggt caatgttgca
     6481 ctgaaattta agaaaaaaag ggggaattgt gaatcacaac tttaaagata aaaccccgaa
     6541 taagtaagga agtattgatg caatccatgc ccataaacca ggtattatct ggaacaatgg
     6601 gaaactctat cacttctatt gcgctgtttc gccaaatcct gatagatata ttggtgaaat
     6661 tgaaaacgaa gaaatcaggg gtattacttt tgcattttcc tgaaaaaaga atctcccacc
     6721 agaaatttcc ttgacatatt gggtcgttgc agggataata aataaaacca taacttttca
//
LOCUS       DAORYU010000062         6713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_7951_length_6713_cov_4.340493, whole genome shotgun sequence.
ACCESSION   DAORYU010000062 DAORYU010000000
VERSION     DAORYU010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6713
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7951_length_6713_cov_4.340493"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4869)
                     /locus_tag="PLW07_03120"
     CDS             complement(<1..4869)
                     /locus_tag="PLW07_03120"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HOL49546.1"
                     /translation="METKLKKISWAFFSILLLIFSSNAFCGTPGTVKWSQKYENANIP
                     SIAIGKDGTVYATGGTRLKAINPANGVIIWNFAPGTTFPSSPVILIDGSICVGGSNGT
                     LYVVNKNGTLRWSKGFGSSINYEVATNTDGTIFLAHGNKLYCISMDGEIIWTYTATDN
                     FNSPPVIGIDGSLYLSAAVPRTLYAINPDGTLKWKKQFPWAAGNDYDDPALLGIPAVD
                     LNGLIFVGDSRGYLRGIAPETGTLKYEYGGMDRASMTYQPITTTNGYVFIGCSNTLIY
                     KFPGGITFLPQGAVMSTPAIGADGKLYFGVAYQVNYHQYYFYAVSSSNMGEVWKCYIG
                     HQMLSSPAIGKDGIAYVGTQDGYIYAIYIENTEYQKNSPYPKYRHDNYNSGNSMYNTY
                     IKCQPAVYNYGNVNLGAKSYKTFALTNISNTSYTVGNISISGENHLEGEFFITDDCSG
                     ESILAGQTLYFTVCFRPLLNGKRSAAVRIPFGDKVTVIGLFGNGGGDGQSALVAIVYD
                     GSNGNLVEEAEVKVAGQTGMTDAEGKCVFSPLDAGGYEIEVNKDGYSPQTKSVEILAS
                     SNVYKNIVIYPSDFTEPKITDVSSKYEGTVFYIDGVNFNVEFTVNIDWAGHTPGRVKF
                     ITPKQTIEEPTNASVVKRTFNMGADFGVGGTLKVQAITESGAISEEKEANFVVIKPLP
                     FFPLAVLDMGSYFSYESKFGINWNLFNEGVNGSSIPSDIPLFGGNPVWLKFIPTVSAE
                     VTSDGAAEIGLEFEGMQKEQKIESGNLAGFSFSLYPMVDIEGQYYPSLSDWDWGGYAG
                     IHGKAEFKKSWPFVVMAGPVPVPMYAKASFSLEAEALLGVTSIDPLGLNGKISIEPYV
                     RGSLGAGVDEMLAVEGWIGGGLTMGLQFPEEPTLEELSIFLNGGFTIYAFLFTWENEL
                     LHWEWSLIEKKLVSSKVPVLDIDHPSVAPRDYLQKNNYGMFAGGKILTKSSDARTQYY
                     TLQENVFPHSEPAISSSGQYLYAVWLYDDPQRSSLNRTKLVFSSFDGTTWSEPVAVSD
                     DGTADFHPDIIVFDDGYAAVVWENAKTQLPDDTTFEQMTQNLEICVSFYNPETKTWTP
                     QYSLTDNNYLDRSPKIKGPDKNTLMVIWVSNPDNHITGNTQNPNVIYSTVWDGTNWAV
                     PVTVVDASNYPDFKKPLLGYDFGFRKVRDDWTAGLVYFCFDMDEDLSTINDREIFANE
                     WDSLNLKGWRTNPWIRTNDNIPDGNVRVFTIGNQFEIYFLKDNNICKTSYFLSDNAYE
                     PVVFYTFEGDYSSNISDYKIARHPDGRVAIVWTEPEQFSSDLFAVFYDPVAKLCGKPQ
                     RLTDDSETEHSPVVAFYDHDTLISIYDRVYLDVFEEKMCLSSGKNVRLPIVKSISTDL
                     AFFIHTITEDVSFETGSFVITPLNPQIGSTAEIYITIENTGNTVLSNIPVYFYAGDPD
                     NGGTLIGNTIITSNLVPGETTIVSVEWEVPAVSQPVTIYAVIDPEDTLSGEISSNNTT
                     SIDCIFPDIGSANLTGCFITEDLLFVTGTLQNIGSLPSGQFVVEVHRDSPDGELIYQD
                     NINQLLPNEIRQINFLFNVTDPKNLYLYLIADPQNAVREYDETNNMYVYKLNPSLLMG
                     DINDDGEVDISDV"
     gene            5152..6543
                     /locus_tag="PLW07_03125"
     CDS             5152..6543
                     /locus_tag="PLW07_03125"
                     /inference="COORDINATES: protein motif:HMM:NF013400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49547.1"
                     /translation="MRKYSGIEKSVFNIATILSLLFMFSSLASAADFTFAIGEPTGII
                     KPLLGVNAGPLIETKDGKVFDATKWYRSIGVKAVRIHDFYGYLDMSVLYPDQNADPSK
                     ASSYNFESSDRFFQAILDGGFEPYLRIGDSWSIGSKFSPIKKRAPVNRQNWVHAAVEV
                     VRHYRKMAGSRLRYVEIWNEPDHRKFWDSSPHEFYVLFDETARALKSEFPDLRIGGPG
                     FTAACVLLPKGTLVVESFLDYLEKHHTPLDFLSWHLYSNNPDVFASVARFYRNKLDMH
                     GFRTTESHLTEYSPDERQIPSGITVPELRMGGEGASILTAAWINLQDEHVEAAFFYRG
                     TDLAMDQPFFYGLFRSDGTPKRPAYAFSFWSRMADCSNRLKVLNIDVNNSPLRMLAGR
                     NHAGNTLLLISNPSNEQVSWTISSAGNQQVTGLSIEEISNASESVKKRDVSGNKAIIP
                     AYSVQLVTVKCKN"
BASE COUNT         2042 a         1425 c         1222 g         2024 t
ORIGIN      
        1 cacatctgaa atatccactt ctccatcatc attaatatca cccataagaa gcgaaggatt
       61 taatttataa acatacatgt tattggtttc gtcatattcc ctgactgcgt tctgtgggtc
      121 tgcgataaga tataagtaaa gatttttggg gtctgttaca ttaaagagaa aattgatctg
      181 ccttatttca tttggcagaa gctgattgat attgtcctga tagattaact caccgtcagg
      241 agaatccctg tgcacttcaa ccacaaactg accagaaggc aatgaaccaa tattttgtaa
      301 tgtccctgta acaaaaagta aatcttcggt aataaaacaa cctgtcaaat tcgctgatcc
      361 tatatcaggg aaaatacaat caatggatgt ggtattgttt gaagaaattt ctcctgagag
      421 agtgtcttca ggatcaataa ctgcataaat ggttactggc tgcgatacag caggaacttc
      481 ccattcaaca gatactatag tcgtttcacc aggaacaaga tttgaagtaa taatggtatt
      541 tcctataagc gtgccgccat tgtcagggtc gcctgcataa aaatagacag gaatgttcga
      601 aagcacagta tttccagtgt tttctattgt aatgtaaatt tctgccgtac ttccaatctg
      661 aggattgagt ggagtaatga caaaactacc tgtttcaaaa gacacatctt ctgtaatggt
      721 gtgtataaaa aatgccaggt cagttgaaat tgatttcaca atgggcaatc tcacattttt
      781 ccctgaggat aaacacattt tttcttcaaa aacatcaaga tacaccctgt cataaatact
      841 gatgagggtg tcatgatcat aaaacgcaac aacaggcgaa tgttctgttt cactgtcatc
      901 agtcaatctc tgtggtttgc cacataattt cgctacaggg tcataaaata cagcgaacag
      961 gtcagaactg aactgttctg gttcagtcca tacaattgct actctaccat cagggtgtct
     1021 tgctatttta taatctgaaa tattgcttga atagtcacct tcaaaggtgt aaaaaacaac
     1081 aggttcgtat gcattatcgc tgagaaaata cgatgtttta caaatattat tatctttgag
     1141 gaagtaaatc tcaaattgat ttccgatagt gaaaactctt acgttcccat caggaatatt
     1201 atcgtttgtc ctgatccatg gattggtcct ccatcctttt agattcaatg aatcccattc
     1261 atttgcaaaa atttctctat cattgattgt tgataaatcc tcatccatgt caaaacagaa
     1321 ataaaccaat ccagcagtcc aatcatctct cacttttcta aagccaaaat cataaccaag
     1381 aagtggtttt ttaaaatcag ggtaatttga agcatcaacc acagtcacag gtacagccca
     1441 atttgtgcca tcccacactg ttgaataaat aacatttgga ttttgagtgt tacctgtaat
     1501 atgattgtcg ggatttgaaa cccatatcac cataagagta tttttatcag gccctttaat
     1561 tttggggctt ctatcaaggt aattattgtc tgtcagggaa tactggggcg tccatgtttt
     1621 cgtttcagga ttatagaacg acacgcagat ttcaagattt tgtgtcatct gttcaaaggt
     1681 tgtatcatct ggcaattgtg ttttcgcatt ttcccagaca actgctgcat atccatcatc
     1741 aaaaacaatg atatctggat ggaagtcagc agttccatcg tcagaaactg ccactggttc
     1801 agaccacgtt gttccatcaa aagaagaaaa aacaagtttt gtgcgattta acgaacttct
     1861 ctgcgggtca tcataaagcc agacggcata aagatattgt ccggaagaag aaattgctgg
     1921 ttctgaatga ggaaacacat tttcctgcaa tgtgtaatat tgcgtcctgg catcagaaga
     1981 tttagtaagt atttttccac cagcaaacat tccataatta tttttctgca aatagtcacg
     2041 aggcgcaaca gaaggatgat caatgtcaag aaccggtacc ttacttgaaa ccagtttttt
     2101 ctcaataaga gaccattccc agtgcaatag ttcgttttcc catgtaaaca aaaacgcata
     2161 tatcgtaaaa ccaccattta aaaaaattga aagttcttca agtgtgggtt cttctggaaa
     2221 ctgcaaaccc attgtcaacc caccgccgat ccatccttca acagccagca tctcatcaac
     2281 accagctcca agggaaccgc gcacatatgg ttcaatagaa atctttccat tcaaccccag
     2341 agggtcaatt gaggtaacac cgagcaatgc ttctgcttcc aaagaaaaac ttgcttttgc
     2401 atacatcggt actggtacag gccctgccat cacaacgaat ggccagcttt ttttgaattc
     2461 tgcctttccg tgaatacccg cataaccacc ccagtcccaa tcactgagtg aaggatagta
     2521 ttgcccctca atatcaacca ttggatacag actgaaagaa aaaccagcaa ggttacctga
     2581 ttctattttt tgttcttttt gcataccctc aaattcaagc ccaatttctg cggctccatc
     2641 gctggtgact tctgctgaca ctgttggaat aaacttcaac cagacaggat ttccgccaaa
     2701 aagtggaata tcagaaggaa tgcttgaacc atttacaccc tcattgaata aattccagtt
     2761 tatcccgaac tttgattcat aggaaaaata tgaacccata tccagaaccg caagcgggaa
     2821 aaatggtaac ggcttgatta caacgaaatt ggcttctttt tcttcagaaa ttgctccact
     2881 ttctgtgatt gcttgaacct ttaatgtacc accaacccca aagtcagcac ccatattgaa
     2941 tgttcttttc acaacagaag cattggtcgg ctcttctatg gtttgctttg gcgtaataaa
     3001 cttcactcta ccgggagtat gaccagccca gtcaatattg actgtaaact caacattgaa
     3061 attaacacca tcaatataaa aaacggttcc ctcgtattta ctggaaacat ctgttatttt
     3121 tggttctgta aagtcagaag gatagataac aatatttttg taaacattgc ttgaagcaag
     3181 tatttcaact gactttgttt gaggtgaata accatcttta tttacttcta tttcataccc
     3241 gccagcatca agaggcgaaa atacgcattt cccttcagcg tcagtcatac ctgtttgacc
     3301 tgcaactttt acttctgctt cttcaacaag atttccattt gaaccatcat aaacaatagc
     3361 aacaagggct gattgcccat caccaccacc attgccaaac agaccaatca ctgtaacctt
     3421 atctccaaat ggtatcctga cagcagcgct cctttttcca ttcaacagtg gtctgaaaca
     3481 cactgtaaaa taaagggtct gacccgcaag tatactttca cctgaacaat cgtcagtaat
     3541 aaaaaattca ccttcaagat ggttttctcc tgaaattgaa atatttccca cagtataaga
     3601 agtatttgaa atattggtaa gtgcaaatgt tttataggat tttgctccaa gattaacatt
     3661 tccataattg tacaccgctg gttgacattt aatatatgtg ttgtacatac tattgccaga
     3721 attgtaatta tcgtgcctgt atttaggata tggactgttt ttttgatatt cagtgttttc
     3781 aatatagatt gcataaatat atccatcctg agtacccaca taagcaattc catcctttcc
     3841 aattgcagga gaagataaca tttgatgccc gatatagcat ttccatactt ctcccatatt
     3901 acttgaagaa accgcataaa aataatactg atggtagttt acctgatagg caactccaaa
     3961 atacaatttt ccatcagcac ctatagcagg agttgacatc actgcaccct gcggtaaaaa
     4021 cgttattcct cctggaaatt tgtagatgag tgtattgctg caaccaatga atacataacc
     4081 gtttgtcgtg gtgataggct gatatgtcat tgaagctcgg tccattccac cgtattcgta
     4141 cttcaaagtt cctgtctcag gtgcaattcc tctcaggtaa ccacgagaat caccaacaaa
     4201 aattaaacca tttaagtcaa cagcaggtat accaagtaaa gcaggatcat cataatcatt
     4261 tcctgctgcc cagggaaact gttttttcca cttcaatgtt ccgtcaggat ttattgcata
     4321 cagggtcctt ggtactgctg ctgaaagata taaactacca tctataccaa tcacaggtgg
     4381 actgttgaaa ttatcggttg cagtgtatgt ccagatgatt tccccatcca tagagataca
     4441 gtacagttta tttccatgag caagaaaaat tgttccatcg gtatttgtgg cgacttcata
     4501 attaatagaa gaaccaaaac cctttgacca tctcaaagtg ccatttttat tgacaacata
     4561 cagtgttcca tttgaacccc caacacagat acttccatct atcagaatca caggcgatga
     4621 aggaaatgtt gttcctggag caaaattcca gattattaca ccatttgcag gattgattgc
     4681 cttgagccgt gttccacctg ttgcataaac tgttccgtct ttccctatgg caatagaagg
     4741 tatattggca ttttcatatt tctgactcca tttgacagtt ccaggagtac cacagaaagc
     4801 attagaggaa aaaatcaaaa gcaatataga aaaaaaagcc catgaaattt tttttaattt
     4861 tgtctccact ttatctccct ggtaaaccca agcaaggata ggcgggtaat ttttatcaat
     4921 ctgtatcttc tgtgttttct tcattcaaat cagatttttt atggcactgt tagttcctat
     4981 atttcattat aaccaaaaaa attggcttat caaaattttg aataatacaa gggcaggcgt
     5041 tttcgccatt taatttatat ggtatgattg aaacgataaa cactttgaca gaaaaatatg
     5101 aaatgataaa ctcaaattga aaatttcagc ggctaacgca ggagatatcc tatgagaaaa
     5161 tattctggca tagaaaaatc tgtattcaat atagccacta ttctatccct tttgtttatg
     5221 ttttcatccc ttgccagtgc agcggacttt acatttgcga ttggggaacc gacaggtatc
     5281 atcaagccgc ttcttggtgt aaacgcggga ccccttatag aaacaaagga cggtaaagtt
     5341 tttgatgcta caaagtggta caggagcatt ggtgtaaaag cggtcagaat ccatgatttt
     5401 tatggatatc ttgacatgtc tgttctttac cctgaccaga atgctgaccc gtcaaaagcg
     5461 tcttcgtata atttcgagtc cagtgacagg ttctttcagg caatcctgga tggcggattt
     5521 gaaccctatt tgcgtattgg agattcctgg agcatcggtt cgaaattttc gcctataaag
     5581 aaacgcgctc ctgtaaacag acagaattgg gttcatgctg ctgtggaagt ggtgcgccat
     5641 tatcggaaaa tggctggttc aaggctgaga tatgttgaaa tctggaacga accagaccac
     5701 agaaaattct gggactcgtc tcctcatgaa ttttatgttc tattcgatga aaccgcgcgg
     5761 gcgctgaaat cagaatttcc tgatttacgc atcggtggac cgggtttcac tgcagcatgc
     5821 gtgctattgc caaaaggaac attggtggtg gaatcttttc ttgactatct tgaaaaacac
     5881 catacccctc ttgattttct ttcctggcat ctctattcaa acaaccccga tgtttttgcc
     5941 agtgttgcgc gtttttaccg aaacaaactc gacatgcacg gatttagaac aaccgaaagt
     6001 catctgactg aatacagtcc agacgaacgc cagataccct ctggtattac ggttccagaa
     6061 ttgaggatgg gtggtgaagg tgcttcaatc cttactgctg catggattaa cctgcaggat
     6121 gaacatgtgg aagcagcttt cttctatcgt ggcacagacc ttgcaatgga ccagccattt
     6181 ttctatggcc tgttccgctc cgatggcact ccaaaaagac ctgcatatgc cttttccttc
     6241 tggtcgcgca tggctgactg ctcaaaccgg ctgaaagtgc tgaacataga tgtcaataat
     6301 tctcctcttc gaatgcttgc aggtagaaat catgctggta atactttgct gctgatttcc
     6361 aatccttcca atgaacaggt atcatggaca atatcctctg ctggaaatca acaggttaca
     6421 ggattatcca ttgaggaaat aagcaatgct tctgaatccg taaaaaaacg ggatgtttcc
     6481 ggaaacaaag caattattcc tgcatattct gttcaacttg taacagtgaa atgcaaaaac
     6541 tgattctgca aatcccttaa cctgcaacaa tgtcaagttg ctgtttttgc aatccttcag
     6601 attcagcaat aattttaatt atgccgcttt tttccataga acgaatacag acaatgcatc
     6661 ttccgtgaaa cgcctttctg agatgggagg gaaacggttg tgttgatgtt ggg
//
LOCUS       DAORYU010000063         6699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_7967_length_6699_cov_6.515051, whole genome shotgun sequence.
ACCESSION   DAORYU010000063 DAORYU010000000
VERSION     DAORYU010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6699
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7967_length_6699_cov_6.515051"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="PLW07_03130"
     CDS             complement(<1..736)
                     /locus_tag="PLW07_03130"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOL49548.1"
                     /translation="MNNLSLFLSLPAAVAFFIPIVSRRAKWFPDIVANLTTFVLLLLS
                     LLTLNREIVYKVGNWPAPYGIVLVLDGFSCMMLVIINLIGFLVTLFSVEYIERFYTSK
                     LRYYSLLLLCITGMNGTILSGDIFNLFIFLELALVSSYALVGFGCTARNLEGSFKYLV
                     INLLASYLILLGVALIYGKFATLNMAHISCMLHGGDSNDILVLFIFVLFFVGFGTKSA
                     LVPFHGWVPDAYMSAPAPVSALLAGGI"
     gene            complement(746..1093)
                     /locus_tag="PLW07_03135"
     CDS             complement(746..1093)
                     /locus_tag="PLW07_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HOL49549.1"
                     /translation="MIYTLCFVLFLVGLYGVTCKKNIVKIILGFLIMEHSINLFLILL
                     GWRKNGIAPVLEKGMSADILVKSAVDPLPQAMVIISIVTGLAILVMMVAIAIRLYQKY
                     GTFDITEMNKLKH"
     gene            complement(1090..1608)
                     /locus_tag="PLW07_03140"
     CDS             complement(1090..1608)
                     /locus_tag="PLW07_03140"
                     /inference="COORDINATES: protein motif:HMM:NF015970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HOL49550.1"
                     /translation="MKTDKKSPGMSLIVKNTARTVMGLIFLFGIYLTLYGHISPAGGF
                     SGGVVFACTFILMTLAFGKDVVLRFLGKTTAYILNCSAAVLFLLIACSGVFFGGNFFE
                     NVIEKMYPSANFRLFSAGFIPFYNLLIAINIGAGLFMVFIIFSVRRVVEMDGKRKLIT
                     TEFFDIKEGKIQ"
     gene            complement(1595..2167)
                     /locus_tag="PLW07_03145"
     CDS             complement(1595..2167)
                     /locus_tag="PLW07_03145"
                     /inference="COORDINATES: protein motif:HMM:NF024641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HOL49551.1"
                     /translation="MVELLVSMLIFFMIIGAIIALETTDLLSAVISIGAIGSVASICY
                     LFVGAPDIASTQLVVEVLTIIILIRATIRHDLITIDGDKEFFGLTVTLGLIFVLFLFG
                     FRALSNLFEFGNPAISQFAEAPSNRYLFSGFQETGANNILSAILFDYRGLDTFAQLGV
                     LFAGIWGAFAIMRVQSRRKKTGKGESNEDR"
     gene            complement(2213..4003)
                     /locus_tag="PLW07_03150"
     CDS             complement(2213..4003)
                     /locus_tag="PLW07_03150"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOL49552.1"
                     /translation="MDTVIYYLIGIPFIAGLVCLILPCKWKNLIWAVTFDVCLVLFGY
                     FLYCFDGRPVLTGNLFVWDGLTNVTLFGISFFGLMISLYSFNLTGDFLNRYYGLLLLT
                     VAAGIGVAVSNNLLLMAVFWGILAITLYLMINLGGPEAAYPAKKTLIMAGGSDSFLIL
                     GIAIVWMLTGVLNITDMHIEIDRTLAAFGFLCFVVAAFTKAGAMPFHSWIPEIAEKAP
                     VSVMAFLPASIDKLLGIYLLARICLNIFSYHETMWFLLRLAGSLTIVCAVFMALIQHN
                     MKKLLAYHAVSQVGYMVLGIVSANPVGIAGGLFHMMNHAIYKCCLFLGAGSVEDRART
                     TDLDNLGGLAGVMPITFFSFLFASFAISGIPPFNGFFSKWMIYQGLVEAGKSGEPSWI
                     LWIVMALIGSGLTLASFMKIIHAVFLGQKSSGIKEKNIKEVHWTMFVPTLVLSLLCLC
                     FGLFYFQVPVVKFLMPIMRIDGIEHLLNLELPLFLIIVGIILGLIVYPLLVPKKKRIA
                     PAFIGGETQEENMRVSGTEFYNAIKDMRFLKTMYIAAEKKFTDLYEVMKSIVSYFTEG
                     FRILHTGILGTYLTWILFGMLVFLYIFIKW"
     gene            complement(4004..5206)
                     /locus_tag="PLW07_03155"
     CDS             complement(4004..5206)
                     /locus_tag="PLW07_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HOL49553.1"
                     /translation="MKKVIVPIGPYHPLFEEPEYFALYCDGERVVDIEWIAGYNHRGI
                     EKLSESKHWDQVTFLIERICGICSTSHPVAYCNAVEDLARIEIPERAKYIRSIVGELE
                     RLHSHLLWVGLAGHFLGYNTVFMWAWKYREPILDIMEMVTGNRNHYAMMKIGGVRRNI
                     ENEDIDIIKKILDELKGKIEMLTAAVLDDPVLAARLKGVGVLTKQNIKDYGAVGPIAR
                     ASGVDIDIRRDSPHAAYNLVEWKVITCQDGDVFSKAVVRLLECFESIKIIEQCLGKLK
                     KIKGEIEVEVREIPPGEGIGRYEAPRGECFHYVRSDGTSRPVRHKIRAPSYVNIPTFK
                     ASCIGETIADVTIILAGVDPCYCCTERAITVYDVDTRKKLFDGKDLIKLSQEKTRKIR
                     KQLGFKEK"
     gene            complement(5203..5643)
                     /locus_tag="PLW07_03160"
     CDS             complement(5203..5643)
                     /locus_tag="PLW07_03160"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOL49554.1"
                     /translation="MDIIEIIKNEFGSKIKSVLPHNEKRIYIEIDKNDLIDVARFVFQ
                     DMDARFITVSAVDNLENMEILYHFSCDPDGIVISLRVFIDRNKLLVESITSIVKGAAN
                     IEREIYELLGIKFLNHPGLKRFLTDEFPEGFYPLRKDSPRKAEQ"
     gene            complement(5650..6072)
                     /locus_tag="PLW07_03165"
     CDS             complement(5650..6072)
                     /locus_tag="PLW07_03165"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HOL49555.1"
                     /translation="MALDLKTKALKKSLFVFHLSTGSCNNCDIEILDCLTPRFDIERF
                     GMVLVGSVKHADVLLVTGIGTIKNAKRIRKLYEQMPKPGFVVAIGACACTGGIFKEGY
                     QMAGPIDKIIPVDVYIPGCPPKPEAMIAGIVKLLEKIK"
     gene            complement(6085..>6699)
                     /locus_tag="PLW07_03170"
     CDS             complement(6085..>6699)
                     /locus_tag="PLW07_03170"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOL49556.1"
                     /translation="DLFIFIYLMMIPSITIFLAGAASGNVLASVGAGRELRLLLSYEL
                     PFILALLVPVVKYNSAQISEIMNHQMMHKMNIGDISGFIAFIVGLLAFQGKIGYVPFD
                     VSEAETEIAGGTFVEYSGLPLALFRLMKWILLSIGVTFFVIYFLGGFHNPVTGILKYL
                     VVLLLIVLVKNTNPRLRTDQILRFSWGWLTMFGFFALILSLGGF"
BASE COUNT         2305 a         1534 c          994 g         1866 t
ORIGIN      
        1 taattcctcc tgcaagaagc gcagacacag gcgctggtgc tgacatatat gcatcgggaa
       61 cccacccatg gaatggaaca agcgccgatt ttgtgccgaa gccaacaaaa aaaagaacaa
      121 agataaacag cacaagaata tcattgctat cgccgccatg caacatacag gatatatgag
      181 ccatattcaa cgttgcaaat tttccatata tcagagcaac accgagcaga atcaaataag
      241 aagcaagaag attgataaca agatatttga acgacccttc aagatttctg gcagtgcaac
      301 caaatcctac gagagcataa gacgaaacaa gagcaagttc aagaaagata aaaagattaa
      361 aaatatcccc tgacaaaatg gtaccattca ttcctgttat acacagaagt agcaaggaat
      421 aatagcgaag tttggaagtg taaaatctct caatgtattc tacagaaaat aatgtaacaa
      481 gaaatcctat aagatttatt atgaccagca tcatacagct gaaaccatca agaactaaca
      541 caatcccgta cggagcaggc cagttaccaa ccttatacac aatttcgcgg tttagtgtca
      601 gtaaagaaag cagtagcaaa acaaatgtgg tcagattagc aacgatatca ggaaaccact
      661 ttgcccttct actcactatt ggaataaaaa acgccactgc tgccggcaga gacaaaaata
      721 atgaaagatt attcataata cattcctaat gtttcagttt gttcatttct gttatatcaa
      781 aagttccata cttctgatag agtcgtattg caattgcaac catcattacc agaattgcaa
      841 gtccagtaac aatagaaata atcaccatcg cctgcggcaa tggatcaaca gcacttttca
      901 ccagtatatc agcactcata cctttttcca gaacaggcgc tattccattt tttctccagc
      961 ccagaagaat taaaaataaa ttgatgctgt gttccataat tagaaaaccc aatataattt
     1021 ttacgatatt tttcttacat gttaccccat acaaacccac aaggaacaaa acaaaacaca
     1081 gggtataaat cattggattt ttccttcttt tatatcaaaa aattctgttg taataagttt
     1141 tcttttacca tccatttcaa caacccttcg tactgaaaaa attatgaaca ccataaacaa
     1201 accagcacca atattaattg ctatcaaaag attataaaaa ggtatgaatc ctgcgctgaa
     1261 aagtcgaaag tttgccgacg gatacatttt ctcaataacg ttttcaaaaa agttcccccc
     1321 aaagaagaca cctgaacagg caatcaacag aaacaacact gccgcagaac aattgagaat
     1381 ataggcggtg gttttaccaa gaaaccttaa cacaacgtcc tttccaaatg caagtgtcat
     1441 cagtatgaat gtacatgcaa aaacaacccc acccgaaaaa ccacctgcag gacttatgtg
     1501 gccgtaaagc gtaagataaa taccaaaaag aaaaataagt cccattactg ttctggctgt
     1561 atttttcact attaaactca tacctggaga ctttttatct gtcttcattg ctctcacctt
     1621 tacctgtttt ctttcttcta ctctgcactc tcatgattgc gaaagcgccc catatgccgg
     1681 caaataacac gcctaattgg gcaaaagtat caagccccct ataatcaaaa agaattgctg
     1741 atagtatatt gttcgctcct gtttcctgaa atccagaaaa aagatatcta ttagatggtg
     1801 cttcagcaaa ttgggatatt gcaggattcc caaattcaaa aagattgcta agtgccctga
     1861 agccaaacaa aaataagaca aaaatcaaac caagcgtaac agtcagacca aaaaattctt
     1921 tatcgccatc aattgtgata agatcgtgcc tgattgttgc cctgatcagg ataataattg
     1981 ttagaacttc aacaacaagt tgcgtgctgg cgatatcagg agcaccaaca aacaggtagc
     2041 atatactggc aacactgcca atcgctccaa tagatatcac tgctgacagc aagtcagttg
     2101 tttcaagtgc aataatcgct cctataatca tgaagaaaat caacatgctg acaagcaatt
     2161 ctaccatttc tcatctctcc agttttctaa aatgttgcat cacttgcatc tatcaccatt
     2221 taataaaaat gtataaaaat accagcatac caaacaatat ccaggttaaa tatgtaccaa
     2281 gtatgcctgt atgaagtatc ctgaaaccct cagtaaaata cgaaacaatt gatttcatta
     2341 cttcgtaaag gtccgtaaat tttttttcgg ctgcgatata cattgttttc aaaaacctca
     2401 tatccttgat agcattataa aactctgttc ctgacactct catattttct tcctgtgtct
     2461 cgccaccgat aaaagcaggt gcaattcttt ttttcttcgg taccaataat ggataaacaa
     2521 taagtccgag tattatgcca acaataatta aaaacaaagg tagttccaga ttaagtagat
     2581 gttcaatacc gtcaattctc ataatcggca taagaaactt taccacgggt acctgaaaat
     2641 aaaataagcc aaaacacaaa cataatagag ataaaacgag agttggaaca aacatcgtcc
     2701 agtgtacttc cttgatattt ttttctttaa tacctgacga cttttgaccg aggaaaacag
     2761 catggataat tttcataaaa cttgccagtg tcagaccact tccaatcagt gccattacaa
     2821 tccacaaaat ccaagatggt tcaccacttt tccctgcttc aaccagcccc tgataaatca
     2881 tccactttga aaaaaatcca ttgaatggcg gtattccact gattgcaaaa gaagcgaaca
     2941 gaaaactgaa aaaagtaatt ggcatcacac ctgccagacc gccaagatta tcaaggtctg
     3001 tagttcttgc cctatcctcc acagaccctg cgccaaggaa aagacagcac ttataaatgg
     3061 catgattcat catatgaaaa aggcctccgg caattccaac aggatttgca ctcacaatac
     3121 ccagcaccat atatccaacc tgacttacag catgatacgc aagaagtttt ttcatattgt
     3181 gttgaatcag cgccataaaa accgcacaca ctattgttaa ggatcctgcc aatctaagta
     3241 aaaaccacat ggtttcatga tacgaaaata tattcagaca aatccttgcc agtagatata
     3301 ttccaagcag tttatcaatt gaagcaggaa gaaaagccat cacagaaact ggcgcctttt
     3361 cagcaatttc aggtatccag ctatgaaacg gcattgcccc tgcttttgta aaagcagcaa
     3421 caacaaaaca taaaaacccg aatgcggcga gcgtcctatc aatctcaata tgcatgtcag
     3481 tgatattcag aaccccggtt aacatccaga cgattgcgat accaagaatc aaaaaggaat
     3541 cacttccacc agccatgata agagtttttt ttgctggata cgcagcttct ggtccgccaa
     3601 gatttatcat tagataaagt gtgattgcaa gaattcccca gaacactgcc atcaaaagta
     3661 aattattgga aactgcaact ccaattcctg cagcaactgt caataataac aatccataat
     3721 acctgttaag aaaatcaccg gtcagattaa aactgtacaa actgatcatc aatccaaaaa
     3781 aacttatccc gaaaagtgta acatttgtca aaccatccca cacaaataaa tttcctgtta
     3841 acaccggtct tccatcaaaa cagtaaagaa aataaccaaa caagacaaga catacatcaa
     3901 atgtgacagc ccatattaaa tttttccact tacagggcaa aatcaaacaa accaatcctg
     3961 caataaacgg tattccgata agataataaa tcaccgtatc catctatttt tccttaaacc
     4021 ccagttgttt cctgattttt cttgtttttt cctgagacaa ttttatcagg tctttcccat
     4081 caaaaagttt ctttcgagta tcaacatcat acacagttat agcacgttca gtacaacaat
     4141 aacagggatc aactcctgca agaataattg tcacatcagc aatagtttct ccaatacaac
     4201 ttgccttaaa tgttggtata ttcacataac tgggcgctct gattttgtgc ctaacaggac
     4261 ggcttgttcc atcactcctc acataatgaa aacattcacc ccttggcgct tcataacgac
     4321 caattccttc tccaggtgga atttctcgaa cttcaacttc aatttcaccc tttatttttt
     4381 tcaatttgcc aaggcattgt tcaataattt tgatggattc aaagcattcc agcagcctga
     4441 caactgcttt tgaaaaaaca tcaccatcct ggcaggttat aactttccat tcaacaagat
     4501 tgtacgcagc gtgagggcta tcccttctta tgtcaatatc aacacctgaa gcccgcgcta
     4561 tgggacctac tgcaccataa tcttttatat tctgttttgt caatacgccc acacccttaa
     4621 gccgtgccgc aagaacagga tcgtcaagga cagcagcagt aagcatttca atttttcctt
     4681 tcagttcgtc aagaatcttt tttataatgt cgatatcctc attttcaata tttcttctca
     4741 ctccaccaat tttcatcatt gcataatgat ttctatttcc agtaaccatc tccattatgt
     4801 caagaattgg ctctctgtac ttccacgccc acatgaagac agtgttataa ccaagaaaat
     4861 gtcctgccaa tcccacccac aataaatgac tgtgcaatct ttcaagttct ccgacaatac
     4921 tgcgaatgta ttttgccctt tctggaattt ctattctcgc aaggtcttca acagcattac
     4981 aataagcaac cggatgagat gttgaacaga tgccacatat cctttcaatt aaaaaggtca
     5041 cctggtccca gtgcttactc tcagaaagct tctcaattcc tctgtgattg taaccggcta
     5101 tccattcaat atctacaact ctttctccgt cgcaataaag agcaaaatac tctggctcct
     5161 caaaaagagg atgatatgga ccaattggta caataacctt tttcattgct ctgccttcct
     5221 tggactgtcc tttcttaaag gataaaaccc ttctggaaat tcgtctgtta aaaatctttt
     5281 taatcccgga tgattcaaaa actttattcc aagtagttcg tagatttctc gttcaatatt
     5341 cgctgcacct tttacaatag acgttattga ttcaacaaga agcttatttc tatcaataaa
     5401 aacacgcaat gaaataacaa ttccatcagg atcacaggaa aaatgatata aaatttccat
     5461 attttcaaga ttatcgactg ctgaaacagt gataaacctt gcgtccatat cctgaaaaac
     5521 aaatcttgca acatcaatca ggtcgttttt atctatttca atataaatcc gtttttcatt
     5581 atgaggaaga acgcttttaa ttttacttcc aaattcattt tttattattt caatgatatc
     5641 catgttttct cacttaattt tttcaagcaa tttcacaatc ccagcaatca tcgcctctgg
     5701 ttttggtgga catcctggaa tataaacatc cactggaata attttgtcaa ttggtcctgc
     5761 catttgatat ccttccttaa aaataccacc tgtacacgca catgcaccaa ttgcgacaac
     5821 aaaaccaggt tttggcatct gctcatagag ttttcgtatc ctttttgcat ttttaatcgt
     5881 tccaatccct gttacaagca agacatccgc atgcttcaca ctgcctacaa gcaccatacc
     5941 aaatctttca atgtcaaatc ttggagtaag gcaatcaaga atttcaatgt cgcagttatt
     6001 acaggaacct gtcgaaagat ggaacacaaa taacgatttc tttaatgcct ttgtttttaa
     6061 gtcaagagcc accttttttc tcccttaaaa cccacccaat gacagaatca gggcaaaaaa
     6121 tccaaacatt gtcaaccatc cccatgaaaa tctcaaaatc tgatctgtcc tcaatcgtgg
     6181 gtttgtattt ttaaccagaa caatgagcaa aagaaccact aaatatttta aaattcctgt
     6241 caccgggttg tgaaagccac ccagaaaata aatgacaaaa aatgtcacac caatggaaag
     6301 cagaatccat ttcatcagtc tgaacaatgc cagaggcagt ccagagtact caacgaatgt
     6361 accgcccgca atttctgttt cagcttcaga cacatcaaaa ggaacatatc cgatttttcc
     6421 ctgaaaagca agcagtccca caataaacgc aataaatcct gaaatgtccc ctatattcat
     6481 cttatgcatc atttgatgat tcataatttc agatatttgt gcagaattat attttacaac
     6541 aggcacaagt aatgccagga taaacggaag ttcataactt aacaataacc taagttccct
     6601 tccagcacca acagaagcaa gaacattacc acttgctgca ccagccagaa aaattgtgat
     6661 ggaaggaatc atcatcaaat atataaatat gaacaggtc
//
LOCUS       DAORYU010000064         6593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_8133_length_6593_cov_4.058428, whole genome shotgun sequence.
ACCESSION   DAORYU010000064 DAORYU010000000
VERSION     DAORYU010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6593
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8133_length_6593_cov_4.058428"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /locus_tag="PLW07_03175"
     CDS             complement(<1..1126)
                     /locus_tag="PLW07_03175"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOL49557.1"
                     /translation="MKIILFLTATLLLAGCASVPLETARDTAKKFYIQGLLYETDGNL
                     EDAFIMYKKALQQDSDSSFLFGKIGTILLKEKKFKEAEKAFLKAIALNKNEPENFLAL
                     GLTYYYMKSYGDAIKYFEKGLKIKDFPSYRMVLCDLYVLTNQYDKALTSYKILIEKFP
                     SNFLLHFNCGLLLVKMNKPEETKKYFLEAIKLQPAFEKSYVELASIYLQQNNTEDALK
                     YYKMATEISPEDPVPYEKMVEIYMKQGNWNQVQSILTKVIKENVNSAMINQIAGFISF
                     QNKQYPEAEIYYKRALMIKETSEIWFNLGVVYDKMNKKNEMEQCIRKAIQIDPKNHLA
                     LNYLGYSLLLENKNIDEAFQLIQQAVRLDPDNGAYLDSLGW"
     gene            complement(1236..2111)
                     /gene="hisG"
                     /locus_tag="PLW07_03180"
     CDS             complement(1236..2111)
                     /gene="hisG"
                     /locus_tag="PLW07_03180"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOL49558.1"
                     /translation="MNQKLKLAIPKGSLQESTINLFVNAGFEVSSISRSYYLSFDDQE
                     IEAILIRAQEIPRYVELGVFDAGISGKDWILENRADIIEICELCYAKRGLKPVKIVLA
                     VPENSNIKSVKDLEGKIIATELVNLTRDYLKRHKVNCNVEFSWGATEIKAGYLVDAIV
                     EITETGTTIASHGLRIIDIIMESTPRFFANKNSWKNSWKKNKMENISLLLEGALNASR
                     KVGLKMNTQKKDIPSILKKLPAIKKPTIAPLAAKGWYAIETIIDKKDAKDIIPELKKL
                     GASGIVEYNLNKLIY"
     gene            complement(2108..3079)
                     /locus_tag="PLW07_03185"
     CDS             complement(2108..3079)
                     /locus_tag="PLW07_03185"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012574202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HOL49559.1"
                     /translation="MERKYLDFEKPLEEIEKQILNLQEIKKRKNIDNQLQITQLQQLF
                     REKTKEIYSNLSSWQKVQVARHPQRPHTDDYIAGLMENFYELHGDRICGDDPAIITGI
                     GKFRGISIAVIGHQKGKDTEENIKRNFGMAHPEGYRKALRIMKIAEKFSFPIVTLVDT
                     PGAHPHIEAEERNQALAIANNLYEMSGINTPIIVVIIGEGGSGGALGIGVGDKIMMLE
                     FAIYSVISPEGCASILWKNQKAVMEASESLKLTAPDLIALGLIDEIVPEPQGGAHANP
                     QEMIKTLGDSIEKNLSELRSIDTNILMEKRYQKYRNIGFYKEKQEII"
     gene            complement(3082..3369)
                     /gene="gatC"
                     /locus_tag="PLW07_03190"
     CDS             complement(3082..3369)
                     /gene="gatC"
                     /locus_tag="PLW07_03190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOL49560.1"
                     /translation="MSNIDRKTVDYLAKLARIKIEDGEMEKIILDLKNILDYISQLNQ
                     LNTENIQPANHVLSFTNVCRKDETKSSLSVDQVMKNAPEKIDNLFRVPKII"
     gene            3477..4526
                     /locus_tag="PLW07_03195"
     CDS             3477..4526
                     /locus_tag="PLW07_03195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOL49561.1"
                     /translation="MKINVLLNLGKKPSGAARPIVEFFNNLTMRHDIVFYKAYNPNRK
                     GCEYFLREILGFLMKGSTFTPGWIECKFPVIIVPSYGKKWVRDADITFFRSAHLARDV
                     TTWGKEKGKKVMRVSNIHMLHNPLGIPENIIFIPSSTMVYEKLKTLYPGHKIYKVGNG
                     VNCNFFSCKERVYEKPGSIGMVVYGGKDSGHKGADIGFEVMKRTKEKFPYLKFFAVGL
                     KKQKNIPDFVEFVYGTTSNEMLQFYRKTDIFIYPSLEDAWPNPPMEAMACGCAVVTTD
                     VGGVRDFAKDKETALICNPGDVDEMTVLVEQLINNPEYWAKLATTGRVAIQEFDYALQ
                     AKTLERVFYEILSTD"
     gene            4504..5619
                     /locus_tag="PLW07_03200"
     CDS             4504..5619
                     /locus_tag="PLW07_03200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOL49562.1"
                     /translation="MKFYQPIDYPAERFERFYKNPPYLRLQKRVRGILVFIGQSHYGD
                     FMLSLPFFQALKEMFPHSKIIFVGRRLPGLEKLSEILPFVDIYYEFSARRKREVLFKW
                     FSLFRLCLKEKVDLLIDTQRYSIVNLLLACLPVRYRLGYGTGCFFSNWKFNEKGRKKN
                     HDIFQLLSLCRVLGKSDIKLYVDINFPEYYENKVRPIISKSEKWISVFPGASEKVKRW
                     PVDRFAAVADALDEMGYSILLLGSSKEKNLLAQVKEKMRYQPVIPVLMDDEFGKNPVY
                     AALFCSKSKLCISNESGGVHFAGLVNTPIVGIYGLKNPVKWGPLSHKSIALYKNFPCS
                     PCKMRKAKLACSYNRKCLLDISVEEVIEACKKCLSET"
     gene            5623..>6593
                     /locus_tag="PLW07_03205"
     CDS             5623..>6593
                     /locus_tag="PLW07_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49563.1"
                     /translation="MKNLLLISYSFPPARNVGALRPRGIAKYLPYYGWKVFVLTPLLG
                     IPRDSQYNMVETYPPFRERMGYLRKKLYFDFSQIISHPPLYKIFLSNFSEIVPFPDTK
                     KGWVVHGVYAGKYVIEKESISAIISTCSPISCHTIAHKLKKIFPQLVWVADFRDLWAH
                     NPFHKHTRPTQFLKETVEKKLLENADFITTVSEPLADHLRRFHNKETFSIPNGFDPDE
                     YNFDAQVDQKFSITYTGSIYPEERDPELLFIALRKLISGDSRIEEDIEVNFFCRYNAA
                     LDELIRKYNLDKVVHQNGIVARENVIKIQKSSHILLLLLPEDKGVYTG"
BASE COUNT         1918 a         1128 c         1243 g         2304 t
ORIGIN      
        1 cccagccaag gctgtcaaga tatgcgccat tatcagggtc aaggcgaact gcctgttgaa
       61 ttaactgaaa tgcttcatca atatttttat tctcaagcag taacgaatat ccaagataat
      121 taagtgcaag atggttttta ggatcaatct gtatcgcctt tctaatacac tgttccattt
      181 catttttttt gttcattttg tcgtaaacga ccccgaggtt gaaccatatt tcggatgttt
      241 cttttatcat cagtgcgcgt ttataataaa tttcagcttc tggatattgt ttgttttgaa
      301 aacttatgaa acccgctatt tgattgatca ttgcagaatt cacattttcc ttgattactt
      361 ttgtcaaaat tgactgtact tgattccagt tgccctgctt catataaatt tcaaccattt
      421 tctcataagg aacaggatct tccggagata tctcggttgc cattttataa tatttcaggg
      481 catcttcagt attgttttgt tgcaggtaga tggaagctaa ttcaacatag ctcttttcaa
      541 aagcaggttg taattttatc gcttcaagaa aatatttctt ggtttcttct ggtttattca
      601 ttttcaccag aagtaatccg caattgaaat gaagcaggaa atttgagggg aatttttcaa
      661 tcagaatttt gtaagatgta agtgctttat catactggtt ggtgagaaca tacaaatcac
      721 acaggaccat tctgtatgat ggaaaatctt ttattttcaa ccctttttca aaatacttga
      781 tggcatcacc ataggatttc atatagtaat atgtgagtcc taatgccagg aaattttcag
      841 gttcgttttt gttgagagcg attgccttca agaacgcttt ttctgcttct ttgaattttt
      901 tttctttcaa caaaatcgta ccgattttcc cgaacaaaaa actactatct gaatcttgct
      961 ggagtgcctt tttatacatg ataaacgcat cttcaaggtt tccatcagtt tcatagagaa
     1021 gaccctggat gtaaaacttt tttgccgtat ccctggctgt ctccaatgga accgatgcac
     1081 atcctgcaag aagaagtgta gcagttagaa aaagaattat tttcatttat ctttttcttt
     1141 tcttgtgcct gagggcgcgt agcctctttt ttcttttgtg tgttttaatt tttctttttt
     1201 ttctttttct tccacacggc atgtgacctc tcctgttaat agatgagttt atttagatta
     1261 tattccacaa ttccggacgc accaagtttt ttcagttcag gaatgatatc ttttgcatct
     1321 tttttatcga tgatggtttc aatggcatac cacccttttg ctgcaagagg ggcgatagtg
     1381 ggctttttaa ttgctggcaa tttttttaga atcgacggaa tgtctttttt ctgtgtattc
     1441 atcttcaatc caacttttct gcttgcgttc agggctcctt caaggagtaa tgaaatattt
     1501 tccattttgt ttttcttcca tgaatttttc caggaattct tattagcaaa gaaacgcggg
     1561 gtggattcca tgatgatatc aataattctc aatccgtgag aagcaatagt tgttccagtt
     1621 tcggtgattt caacaatggc atcaacaaga tagccggctt ttatttctgt tgctccccag
     1681 gaaaattcca cattacaatt taccttgtgc cttttgaggt aatctctggt tagattgacg
     1741 agttctgttg cgataatttt cccttcaaga tcctttacag atttaatatt tgaattttcc
     1801 ggtacagcca gaacgatttt gacaggtttt aatccacgct ttgcataaca cagttcgcat
     1861 atttctatta tatctgcccg gttttcaagg atccagtcct tccctgatat tcctgcgtca
     1921 aaaaccccta attctacata ccttggaatt tcctgagccc ttatcaggat tgcttctatt
     1981 tcctgatcgt caaacgaaag ataataactt ctgcttatgc ttgaaacttc aaatcctgca
     2041 ttgacaaaaa gattgattgt ggattcttga agactgcctt ttggtattgc aagtttcaat
     2101 ttttgattca tatgatttcc tgtttttctt tgtaaaaacc gatatttcta tatttttgat
     2161 atcgtttttc cattagaata ttggtatcga tggaacgtaa ttcactcaga tttttttcta
     2221 tgctatcgcc gagagttttt atcatttctt gaggatttgc atgcgctcca ccctgaggct
     2281 ctggaactat ttcatctatt aaaccgagag caattaaatc aggtgcagta agtttcaaag
     2341 attcagatgc ttccatcact gctttttggt ttttccagag tattgaagcg cagccctccg
     2401 gagatattac agagtatatt gcaaattcaa gcatcatgat tttgtcaccg acccctatgc
     2461 cgagggcgcc cccgcttccc ccctctccaa tgattacgac gataattgga gtatttattc
     2521 cagacatttc ataaagattg tttgcaatcg caagtgcctg atttctttct tcagcctcta
     2581 tgtgaggatg tgcaccaggg gtatctacca gtgttacaat gggaaatgag aatttttcgg
     2641 caattttcat tattctgagt gcttttctat atccttccgg gtgagccata ccaaagtttc
     2701 ttttgatatt ttcttcagtg tctttacctt tttgatgtcc aataacagca attgaaatcc
     2761 ctctaaattt tccaatacca gtgataatcg ccgggtcatc tccacaaatt cgatcaccat
     2821 ggagttcata gaaattctcc atcaagccag caatataatc atctgtatga ggtctctgag
     2881 gatgtcttgc gacctgaact ttctgccatg atgacagatt tgaataaatt tcttttgttt
     2941 tttcacgaaa cagttgttga agctgtgtta tctgaagttg attgtcgata ttttttcttt
     3001 tttttatttc ctgaagatta agtatctgtt tttctatttc ttcaagtggt ttttcaaaat
     3061 caagatattt tctttccatt tttaaattat ttttgggacc ctgaaaagat tatcgatttt
     3121 ttcaggagcg tttttcatga cctggtcgac tgaaagggat gattttgttt cgtcttttct
     3181 gcaaacattg gtaaacgaca atacgtgatt tgccggttga atgttttcag tattcaactg
     3241 gttgagctga gaaatataat ccagtatgtt tttcaggtca agtatgatct tctccatttc
     3301 gccatcttca atttttatcc ttgcaagttt tgcaagatag tccactgttt ttctgtcaat
     3361 gtttgacata ttgcaccttt tgtaacatta tacagggaaa aaatcttttg gtcaaagaat
     3421 tttttttgtt caagttaatc tgtgatatta ttttgtaaaa cattttgttg acacaaatga
     3481 aaatcaatgt actgttaaat ctgggcaaaa aacccagtgg tgcagcaaga ccgattgttg
     3541 aattctttaa taatttgact atgcgccatg atattgtttt ttataaggca tataatccaa
     3601 acaggaaagg ttgtgagtat tttttaagag aaattcttgg ttttttgatg aaaggttcaa
     3661 cgtttacccc tggatggata gaatgtaaat ttccagtaat tattgttcca tcgtatggga
     3721 aaaaatgggt aagagatgca gatattacgt tttttcgttc agcacatctt gccagagatg
     3781 ttactacctg gggcaaagaa aaaggaaaaa aagtaatgag ggtttctaat attcacatgt
     3841 tgcacaatcc tttaggtatt ccagagaaca tcatctttat tccttcttcc acaatggtgt
     3901 acgagaaatt aaaaacgttg tacccggggc ataaaatcta taaggtgggt aatggtgtta
     3961 attgcaattt tttttcctgt aaagaaaggg tttatgaaaa gccaggttct attggaatgg
     4021 ttgtatatgg aggcaaagat tcaggacata aaggtgcgga cataggtttt gaagtaatga
     4081 agaggacaaa ggaaaaattt ccttatctaa aattttttgc tgtcgggtta aaaaaacaga
     4141 aaaacattcc tgactttgtt gagtttgttt atggaacaac gagcaatgaa atgttgcagt
     4201 tttatagaaa aacagatatt tttatttatc cgtctttaga ggatgcatgg ccaaatccgc
     4261 caatggaagc aatggcgtgc ggttgtgcag ttgtaacaac agatgttggc ggagtaaggg
     4321 attttgcgaa agataaagaa acagcattga tttgtaaccc aggagatgtg gatgaaatga
     4381 ccgtactggt tgaacaactt attaataacc ctgaatactg ggcaaaactg gctacgactg
     4441 gtagggttgc tattcaagag tttgattatg ctttacaggc aaaaacactg gaaagggttt
     4501 tctatgaaat tttatcaacc gattgattat cctgcagaac gatttgaaag gttttacaaa
     4561 aatccgccat atcttaggtt acaaaaacgt gtaagaggta tacttgtttt cataggacaa
     4621 tcccattatg gagattttat gctttcctta ccattttttc aggcgctgaa agagatgttt
     4681 ccacattcaa aaattatttt tgttggacgt aggttaccgg gtctcgaaaa actttctgaa
     4741 attttgccat ttgttgacat atattatgaa ttcagtgctc gaagaaaaag agaggtactg
     4801 ttcaaatggt tttcattgtt tcgactttgt ttgaaagaaa aggttgatct tttaattgat
     4861 actcagagat attctattgt taatctatta cttgcctgtt tgcctgttcg atacagactt
     4921 ggttatggaa caggttgttt tttctcaaac tggaaattta atgaaaaagg tagaaaaaaa
     4981 aatcatgata tatttcaact tctgtctctt tgtagggttc tggggaaatc tgacataaaa
     5041 ttgtacgttg atattaattt tcctgaatat tatgaaaata aagtcagacc aattatttct
     5101 aaatccgaaa agtggatttc tgtttttcct ggcgcatcag aaaaagtaaa acgctggccc
     5161 gttgataggt ttgctgcagt ggcagatgct ctggacgaaa tgggctattc tattttactt
     5221 ctgggtagtt cgaaggaaaa gaatctgttg gctcaggtca aagaaaaaat gagatatcaa
     5281 ccggttattc ctgttctgat ggatgatgaa tttgggaaga atcctgttta tgcggcttta
     5341 ttttgttcaa aaagcaaatt gtgcatcagt aatgaaagtg gaggagtaca ttttgcaggg
     5401 cttgttaaca ctcctattgt tggtatctac gggctaaaaa atcctgtaaa atggggacct
     5461 ttaagtcata agagtattgc actttacaaa aattttccat gttctccctg caaaatgaga
     5521 aaggcaaaac ttgcctgttc atacaacagg aaatgtctgc ttgatatatc agtagaagaa
     5581 gtgattgaag catgtaaaaa atgtttaagt gagacatgaa agatgaaaaa tctacttctt
     5641 atttcctact cttttccacc ggccagaaat gttggcgcat taagaccaag aggtattgca
     5701 aagtatctgc catattatgg atggaaagtt tttgttctaa ctccattact gggcataccg
     5761 cgtgattctc aatataacat ggttgaaaca tatccacctt tcagggaaag aatggggtat
     5821 ttgagaaaaa aattatattt tgattttagt cagattatat cccatccccc actatataaa
     5881 atttttttat ctaatttttc agagattgtt ccattcccag atactaaaaa gggatgggtt
     5941 gtacatggtg tttatgccgg gaaatatgtg attgagaaag aatcaatttc tgctattatc
     6001 agtacatgca gtccgataag ctgtcataca attgctcata aattaaaaaa aatttttccg
     6061 caactggtct gggttgctga tttcagggat ttatgggctc ataacccatt tcataaacat
     6121 acaagaccga cgcagtttct gaaagaaaca gttgaaaaaa aactacttga gaatgcagat
     6181 tttataacca cagtctcaga acctcttgca gatcatctga gacgatttca taataaagag
     6241 accttttcga ttcctaatgg tttcgaccct gacgaatata actttgatgc acaagttgac
     6301 cagaagtttt ctattacata tacgggaagc atttatccgg aagaaagaga tccagaattg
     6361 cttttcattg cacttagaaa acttatttct ggggattcgc ggatagaaga ggacatagaa
     6421 gtgaattttt tctgcagata taatgccgca ttagatgaac tgataagaaa gtacaatctg
     6481 gataaagtag tgcatcaaaa cggaattgtt gcaagagaaa atgttattaa aatacaaaaa
     6541 tcatcacata ttctgctttt gttgctaccc gaagacaagg gagtgtacac agg
//
LOCUS       DAORYU010000065         6576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_8159_length_6576_cov_5.566784, whole genome shotgun sequence.
ACCESSION   DAORYU010000065 DAORYU010000000
VERSION     DAORYU010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6576
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8159_length_6576_cov_5.566784"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="PLW07_03210"
     CDS             complement(<1..335)
                     /locus_tag="PLW07_03210"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOL49564.1"
                     /translation="MSEKLAINGGKRLIPEGFIKPWPPIDDVDIKYVVDSLKMPFHAF
                     GPNCSAFEKEFAAWNGNKFAITTNSGTAALHMAVAACDCGAGDQIIVTAYSWSSSATC
                     ILHHNCIPV"
     gene            complement(346..1653)
                     /locus_tag="PLW07_03215"
     CDS             complement(346..1653)
                     /locus_tag="PLW07_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49565.1"
                     /translation="MLFAGAGTRKINCKIGDSIMGQLHIRKCQYIMDDLEANSVFLSD
                     GTTKILFISCDLAVLLPDFIARAKQSIEKKTSIPQANIFIFCTHTHTGPNTANLLPDD
                     PINSSYLDELETNLVNLAEEAVSSAIEAKLGYGKGTALIGYNRRVCWMDGSHTMYGDT
                     KKTEFAGIEGPEDPTHCVISIIDKQNNPIAIIHNNACHSTCVENGNFASSDFPGESRR
                     LLRKIFDKELLPMLYLQGASGDLSPWNLMKPENRVSGIQRMKEIGAICASETAKIIAS
                     MDYEQNPVIKIAREKLKIKVRLPEKDEIERATKIVERGEEKESRGSYVLNWSILKLYE
                     EFKNISCEEIPVAAIRIGNCTIATNPYEYYCQFGIDIRRRSPAQITLIAQLADGWYGY
                     CPTIYGVLGGGYSGMTIYWTRHQPEAGYIVADACCRLLYQLFK"
     gene            complement(1668..2429)
                     /locus_tag="PLW07_03220"
     CDS             complement(1668..2429)
                     /locus_tag="PLW07_03220"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOL49566.1"
                     /translation="MNFIKFLGTAGARFVMIKQLRASGGIWFEINDTRMIVDPGPGAL
                     VRVNKSRPSLKVSHLDAIFVSHKHIDHTNDVNVMIEAMTDGGFNKKGIVLCPSEAIEN
                     PPVIFPYAAALVEQVITLNTGSQYQIKNLKLKIPVRLHHQTETYGFILKSDNVPSIGY
                     IPDTEFFPELISAYENTDIIIINVVFTQPRPGIMHLGLPEALDIISGIKPKKAILTHF
                     GMTMLKEKIWEKTRSLSEKFSFDIITASDGMTVEL"
     gene            complement(2443..3702)
                     /locus_tag="PLW07_03225"
     CDS             complement(2443..3702)
                     /locus_tag="PLW07_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOL49567.1"
                     /translation="MSKMAKKNNKKSEKSISSGEKKKKTAPNRNVKSVEVSFSEERFK
                     KAKDQILALGRENKTVFIEDINKFLPIDTPGDKIDELFDELEEMHIEIGDDLFAEKES
                     EEDTRMVLEDLRNPLRAYLKDAGSFNLLTSEEEVEIAKIMEKYKKALQQMCKKRRNGK
                     NLDRIHRYQQEYVKARDRLIQANLRLVISIAKKYSNPKLSLRDLIQEGNIGLMKAVEK
                     FRYKEGFKFSTYATWWIRQAITRAIADHSATIRIPVHMIEKINKLSKAYRWLSQSLDR
                     EPTDEELAKVMNTDSEKIKKIKKSMRPEPVSLDMPVGDEERATVGDFIEDNEESSPLT
                     HTRRTLLREELEKAFEILDEREEKILRLRFGLDRDGYARTLEEVGKYFNLTRERIRQI
                     EGKAIQKLKNAPRASKLKPFLDQINFT"
     gene            complement(3677..5485)
                     /gene="dnaG"
                     /locus_tag="PLW07_03230"
     CDS             complement(3677..5485)
                     /gene="dnaG"
                     /locus_tag="PLW07_03230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOL49568.1"
                     /translation="MPVPSEKIDEIIEKIDILEVVSKYVQLKKTGRNYKGLCPFHPEK
                     TPSFFVSSDKQIFHCFGCGTGGNVLSFLMKIENTSFSEAVALAAEIAGVQIQVSGYSD
                     EADVRRKPILEANKIAMNFFKKSLETSQGNVAIDFLEKRGISQLYREKFCVGYAPPGN
                     GLLDYLNTSKLSIVNFEQAGLVIKKETGYTDLFRHRVIIPIFDHRNNICGFGARSIED
                     QQQPKYLNTSENYVFKKGNLFYGLNWAKEKIKGSGFAIIVEGYFDLIKMHMAGFENTI
                     APLGTALTENHIRTLKRWTDKILLVFDSDSAGTSAAFRSLETILAGGFEAKIGVMPSG
                     FDPEDFLDNYGADALKKLLNQSKDFVDFAFHIGSQKHSIDNPRGRAALIEEILGLIKH
                     IPDSIERSIRIGQLAKIADVDESILAQQLDNIPEKNKSRAFAQVPVQKTQSCAGNHAE
                     KTLIRILFSQPSWANEIKPYLGFLPETISMVVEHCASNEISENNISKILNQINNPDLS
                     GFLTQAIFKENGTENIDVTRKEFYDCVKMLCKKSLENRRRKLQEIIKQKTETGQSCDK
                     ELEELQFCINQANVKNLESILSGQERSVDVQNGEKE"
     gene            5638..6129
                     /gene="lepB"
                     /locus_tag="PLW07_03235"
     CDS             5638..6129
                     /gene="lepB"
                     /locus_tag="PLW07_03235"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOL49569.1"
                     /translation="MIFRIIKTVIGAAILFLVGYYFGLRGVRFYQVISESMLPTLEVG
                     DRLIGAKFEKLDYGDIIACDDPLEKNGIIIKRVVGLEGDLIEVKNGYLYRNREKVEEP
                     YIMEKPDYKVDPVRLKTEEIFVLGDNRNNSSDSSVWGPVQISSVHARLTIRYWPFKKF
                     KVL"
     gene            complement(6185..6454)
                     /locus_tag="PLW07_03240"
     CDS             complement(6185..6454)
                     /locus_tag="PLW07_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOL49570.1"
                     /translation="MNKAELIEAVAKVVTTKKEAAAAVDTVIDSIKASLKKGKPVTLV
                     GFGTFKVVKRKARTGRNPRTGATIKIPAKNIPVFRAGSELKAAVK"
BASE COUNT         1743 a         1350 c         1228 g         2255 t
ORIGIN      
        1 aaaacaggaa tgcagttgtg atgaagaata catgttgcgc ttgaagacca ggaatacgct
       61 gtcacaatga tctgatctcc tgcaccacaa tcacacgcag ccacagccat gtgcagagca
      121 gcagtgccgc tatttgttgt gattgcaaat ttgttgccat tccaggcagc aaattctttt
      181 tcaaatgccg aacagtttgg accgaaagca tgaaagggca tcttgagaga atcaacaaca
      241 tacttgatat caacatcgtc aatcggtggc catggtttta taaaaccttc aggaatcagt
      301 cgtttcccgc catttattgc aagtttttct gacatgtttt gcctcctatt tgaataattg
      361 ataaagtaac ctgcagcaag catcagccac aatataacct gcttctggct gatgtcgtgt
      421 ccagtaaatt gtcattccag aataaccacc acccagtact ccataaattg tcgggcaata
      481 tccataccaa ccatcagcaa gctgcgcaat cagggtaatc tgtgcagggc ttctgcgtcg
      541 tatatcaatt ccgaactggc agtaatactc gtagggattt gtcgcaatgg tacaatttcc
      601 aattcgtata gcagcaacag gaatttcttc gcaggaaatg tttttgaatt cctcatacaa
      661 ttttagaata ctccagttca agacatagct ccctctgctt tctttttctt ctcccctttc
      721 aacgattttc gttgcccttt caatttcatc tttttcaggg agcctgactt taatctttag
      781 tttttcacga gcaattttta tgacagggtt ttgttcgtaa tccatgctgg caatgatttt
      841 tgctgtctct gaagcacata ttgctccaat ttctttcatc ctttgaatcc ctgagacgcg
      901 gttttctggt ttcataagat tccagggaga cagatccccg cttgcaccct gaagatacag
      961 cataggtagt aactctttat caaaaatttt tctgagaagt cgtcttgatt cccctgggaa
     1021 atccgatgat gcaaaattcc cgttctcaac gcaggttgaa tggcaggcat tgttatgtat
     1081 gatggcaatg ggattgttct gtttatcaat gattgaaatc acgcagtggg tcgggtcttc
     1141 tggtccttca atgcctgcaa attctgtttt ttttgtatct ccatacatgg tatggctgcc
     1201 atccatccag cacacccttc tgttgtatcc tatcagtgcg gttccttttc cgtatccaag
     1261 ttttgcctct atcgcagatg agactgcttc ttcagcgagg ttaacaaggt ttgtttcgag
     1321 ttcgtcaaga taactcgaat tgatcgggtc atctggaagc agatttgctg tgttaggtcc
     1381 tgtgtgggta tgggtgcaga agataaaaat gtttgcttgt ggaatagagg ttttcttttc
     1441 aatgctctgt ttcgctcttg caataaaatc aggcaaaagc actgccaggt cgcaactgat
     1501 gaaaagaatt tttgttgttc catcagaaag aaacacagaa tttgcttcaa ggtcatccat
     1561 aatatactgg cactttctga tatgcagttg tcccatgatg ctatcgccga ttttacagtt
     1621 tatttttctg gttccagcgc ctgcaaaaag catttctccc ctctgtgcta aagttcaact
     1681 gtcattccgt cagatgcagt aattatatca aaagaaaatt tttctgacaa tgaccgggtt
     1741 ttttcccata ttttttcctt cagcattgtc attccgaaat gcgtgaggat tgcctttttt
     1801 ggcttaatac ctgaaatgat atcaagcgct tcaggaagac ccagatgcat tattcctggt
     1861 cttggctgag tgaaaacaac attgataatg atgatatcag tattttcata agcagaaata
     1921 agttcaggaa aaaattcagt atcagggata tacccgatag agggaacatt gtctgattta
     1981 agaataaaac catatgtttc tgtttgatga tgaagtcgaa cagggatttt taattttaag
     2041 tttttaatct gatattgcga gccagtatta agggtgatca cctgttcaac gagtgccgca
     2101 gcatatggaa aaatcaccgg tggattttca atggcttctg aaggacatag cacgatgccc
     2161 tttttattga aaccgccgtc agtcatcgct tcaatcatca cattgacatc attggtatgg
     2221 tctatgtgtt tatgtgaaac aaaaattgcg tccagatgag aaacttttaa tgacgggcgt
     2281 gatttattca ccctgacaag ggctcctggt ccagggtcaa caatcattcg tgtatcattg
     2341 atttcaaacc atattccacc tgatgcccgc aactgtttta tcatcacaaa acgggcacca
     2401 gctgttccaa ggaattttat gaagttcaaa cgatagtcca ggttatgtga aatttatctg
     2461 gtcaagaaat ggtttgagtt tggaagcgcg cggtgcattt ttcaatttct ggatggcttt
     2521 cccttcaatc tgtcttattc tttcccttgt cagattgaaa tacttaccga cctcctcgag
     2581 tgttcttgca taaccatccc tgtcaagacc aaacctgagt ctgagaattt tttcctctct
     2641 ttcatcaaga atctcaaatg ctttttcaag ttcttccctc aacagcgttc ttcttgtatg
     2701 agtaagaggg ctggattctt cattatcttc aataaaatct ccaacggttg ccctttcttc
     2761 gtctcctaca ggcatgtcca gtgaaacagg ttcaggtctc attgatttct tgattttctt
     2821 aatcttttca gagtcagtgt tcattacttt tgcaagctct tcgtctgttg gttctctgtc
     2881 taaactctgt gaaagccatc tgtacgcctt gctgagtttg ttaatttttt ctatcatgtg
     2941 tacgggtatt cttattgttg cagagtggtc tgcaatagcc cttgtaattg cctgtcttat
     3001 ccaccatgtt gcataggtgc tgaatttaaa tccctctttg tatctgaatt tttctactgc
     3061 tttcatcaga ccgatattac cttcctgaat taaatctctc agagaaagtt ttgggtttga
     3121 atattttttt gcaatgctga taacaagcct gaggtttgcc tgaatcaatc tatctcttgc
     3181 tttaacatat tcctgctggt acctgtgaat tctgtcaaga tttttaccgt ttcttctttt
     3241 cttgcacatc tgttgaagcg cttttttgta tttctccatt atttttgcta tttcgacctc
     3301 ttcttctgag gtgagaagat taaaagaacc agcgtcttta agataagccc tcagaggatt
     3361 tcgaaggtct tcaaggacca ttcttgtgtc ttcttcgctt tccttttctg caaaaaggtc
     3421 atcgccaatt tcaatatgca tttcctcaag ttcatcaaaa agctcatcaa ttttatctcc
     3481 aggggtatct atgggaagaa atttgttgat atcttcaatg aaaactgtct tattttctct
     3541 gccaagggca agaatctggt cttttgcctt tttgaatcgt tcttcagaaa aagaaacttc
     3601 cacagatttg acattcctgt tcggggcggt tttcttcttt ttctctccag atgaaattga
     3661 tttttcactc tttttattat tctttttcgc cattttggac atcaacgctc ctttcttgtc
     3721 cggacaaaat tgattcaaga tttttcacgt ttgcctggtt aatgcaaaat tgcaattctt
     3781 caagttcttt atcgcaggat tgtccggttt ctgttttttg tttgataatt tcctgcaatt
     3841 tcctgcgacg attttcaagg gatttcttac acaacatttt gacacagtca taaaattcct
     3901 ttcttgttac atcaatattt tcagtgccat tttctttaaa aatcgcctgg gtcaaaaaac
     3961 cactaaggtc tggattattg atttgattca aaatttttga aatattattt tcggatattt
     4021 cgttgctggc gcagtgttcc acgaccatgc tgattgtttc tggcaggaaa ccaaggtatg
     4081 gtttaatttc attagcccag gagggttgac tgaaaagtat tctgatcagg gttttttctg
     4141 cgtgatttcc tgcacaggat tgagttttct gaacaggaac ctgagcaaaa gcccgggact
     4201 tatttttttc aggaatgttg tccagttgct gcgcaaggat actttcgtca acgtctgcaa
     4261 tttttgcaag ttgtccaatc cggatagacc tttcaatgct atccgggata tgttttatta
     4321 gaccaagtat ctcttctatc agggcggcac gtcctctggg attatcaata gaatgtttct
     4381 ggcttccgat atgaaaagca aaatcaacaa aatcttttga ctgattgagt aattttttca
     4441 acgcatcagc gccataatta tcaagaaaat cttcagggtc aaaaccagaa ggcatcactc
     4501 cgatttttgc ctcaaagccg cctgcaagta tggtttcaag gcttctgaaa gcagcagatg
     4561 tgccagcgga atcagagtca aaaaccagaa gtattttgtc tgtccatcgt ttcaaagtcc
     4621 tgatatggtt ttctgtaagg gctgttccca gaggagcgat agtattttca aatccagcca
     4681 tatgcatttt tataagatca aaatatcctt ccacaatgat tgcaaatcca gaacctttta
     4741 ttttttcttt tgcccaattc aatccataaa agagattacc ctttttaaaa acatagtttt
     4801 cagacgtatt caaatacttt ggttgctgct gatcctcaat agatcttgct ccaaaaccac
     4861 agatattatt gcgatgatcg aaaattggta tgatcactct gtgcctgaaa aggtctgtat
     4921 atccggtttc tttttttatc acaagacccg cctgctcaaa attgacaatt gacaatttgc
     4981 tggtattcaa ataatccagt aaaccattac caggcggtgc ataccctaca caaaattttt
     5041 cacgatacaa ttggcttatt ccgcgttttt caagaaaatc aattgccaca ttgccctggc
     5101 ttgtttcgag ggacttcttg aaaaagttca ttgctatttt atttgcttca agaattggtt
     5161 ttcttcttac atccgcttca tctgagtacc cagaaacctg tatctgtacg cctgctattt
     5221 ctgctgcaag cgcaactgct tctgaaaaac tggtgttttc aattttcatc agaaaactca
     5281 aaacattacc acctgtgcca catccgaaac aatggaaaat ttgcttgtca gaacttacga
     5341 agaaagaagg cgtcttttct ggatgaaaag ggcagagtcc cttataattt cttcccgttt
     5401 tttttaattg tacatacttt gataccactt caagtatgtc aattttttca atgatttcgt
     5461 caattttttc agatggaaca ggcatagtga caaaaaaatt tatccttttg gatacagttg
     5521 ttttaaaaaa tttttttatt cattgttgat tagttatacc tgtaagagta tatcggtgtc
     5581 aagacattgc agaaacaggt tattttatta actgttttga tggtgtataa ttattttatg
     5641 attttcagaa ttattaagac agttattggt gcagccattc tttttcttgt tggatactat
     5701 tttgggttgc ggggcgtcag attttatcag gtaatctctg aatcaatgct tccaacactg
     5761 gaagttggag atcgtttgat tggagcaaaa tttgaaaagc ttgattacgg agatatcatt
     5821 gcctgtgacg atcctctgga aaaaaatggc atcattatca agcgagttgt tgggcttgag
     5881 ggtgatctta tcgaagtaaa aaacggttat ttatatagaa atcgtgaaaa agttgaagaa
     5941 ccgtatatta tggaaaaacc cgattataaa gttgaccccg tgcgactgaa aacagaagaa
     6001 atctttgttc ttggagacaa tagaaacaac agttcagaca gcagtgtctg gggacctgtt
     6061 caaatatcct ctgtccacgc cagattaacc atccgttact ggccattcaa aaagtttaaa
     6121 gttctgtaaa aaaagatacg gacctcacag gagatccgta ccttggtttc tttcaataac
     6181 cctattactt aacagcagcc ttcaactcag aacccgctct gaatacagga atgttctttg
     6241 cgggaatctt aattgtggca ccggttcttg gattgcgccc tgttcttgct tttctcttga
     6301 caaccttgaa tgtgccgaac cccaccagcg ttaccggttt cccctttttc aaagatgcct
     6361 taatactgtc aataactgtg tctactgctg cagcggcttc cttttttgta gttaccacct
     6421 ttgctactgc ttcgatcaat tctgctttat tcattcgtct tcacctcctt ccatttattt
     6481 tttttcaata tatcactggc tttcaaactt gtcaagtgct tcagagtatt gactgataag
     6541 cattttggaa ttattgtaaa tgtatgagca aggatt
//
LOCUS       DAORYU010000066         6542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_8206_length_6542_cov_3.968090, whole genome shotgun sequence.
ACCESSION   DAORYU010000066 DAORYU010000000
VERSION     DAORYU010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6542
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8206_length_6542_cov_3.968090"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="PLW07_03245"
     CDS             <1..388
                     /locus_tag="PLW07_03245"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOL49571.1"
                     /translation="EEFQKNSNKSLKNIMKNLLPQNIIEEFLLIGTLSGEIKGNQVTA
                     EQRTILISLLKNFCFNFDSLRGFEQAIVTRGGVSVKEVNPKTMESKIVPGLFFAGEIL
                     DIDGKTGGFNLQAAFSTGFVAGKHAG"
     gene            420..1298
                     /locus_tag="PLW07_03250"
     CDS             420..1298
                     /locus_tag="PLW07_03250"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOL49572.1"
                     /translation="MTIKHITTNQSHAEKNIKFPDIGLSPTFSDTGIGVWIYCDDCIH
                     FMDKMLEKYPDGIFDMIFADPPYFLSNGGITCHAGKMTKVDKGNWDKSRGVEQNHAFN
                     ISWLSRCQKLLKQNGTIWVSGTHHTIYSVGFAMQQLGMKILNDISWEKPNPPPNLSCR
                     YFTHSTETIIWAGKNTRCRHTFNYELVKQLAGGTQMKTVWRIPAPAKEEKLFGKHPTQ
                     KPLKLLERIIIASTNEGDLIFDPFLGSGTTAVAALKLKRKFVGCELEESFVELAIKRV
                     THALHLTTNKLPLDGS"
     gene            1298..1849
                     /locus_tag="PLW07_03255"
     CDS             1298..1849
                     /locus_tag="PLW07_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49573.1"
                     /translation="MKNSGTGGEKTITGLNFEKKVDLQILLSKMPGYELKKIPGKAGT
                     CVFFEGKIVARCFRKNEFYKFLEENDIKWQAVISKKLLPDDALFVIVRETLFIIEVKY
                     QQVPGSVDEKLQTCDFKRKQYLKLVNPLGLKVEYVYVLNDWFKKPQYKDVLDYITSVN
                     CHYKFHQLPLAWLGLPVKQSTDV"
     gene            1959..5270
                     /locus_tag="PLW07_03260"
     CDS             1959..5270
                     /locus_tag="PLW07_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOL49574.1"
                     /translation="MIMIESDNVLKGPFWPEKIRVISVKPIGSNQIKIEAVGLETNHF
                     YNPVLSENDIKDIEIQEGRRFLFSTDGESLFLYLESHRIRNAFQFDPLYAINVSQIDP
                     LPHQIEAVYHYIMQNPRIRFLLADDPGAGKTIMAGLLLKELKYRGLVERTLIIVPGHL
                     KDQWLREMKEKFQENFLIVDRSIMNSEWGTNIFKNRNQVLISMDFAKQDDVIFALKDS
                     RWDLCIVDEAHKMSAYKYGQKISKTQRYNLGEILSPLTNYLIFLTATPHRGDPENFRL
                     LLDLLEPGFFVDTSMLSESIKNKDNPLFLRRLKEDLKDFESVQIFPPRNVETTKYSLS
                     DDEKKLYNAVTEYVEQHFNKALEKEKRNVAFALTILQRRLASSVRAIRKSLERRHRRL
                     TELFQKGQLLSDAEYDENYIEDLEEKERWKKEEELLEKLTSAETLEELNDEIGKLREL
                     VLLAKEVEKKEIETKLNQLKKVMDDEKLQESKTKLLIFTESKDTLEYLVEKLNLWGYS
                     VVYIHGGMNLDERIRAETDFRNRAQIMVSTEAGGEGINLQFCWLMVNYDIPWNPTRLE
                     QRMGRIHRYGQRHEVHIYNMVAADTREGRILEKLFEKLYQIRRHLGSDRVFDVIGEVL
                     VGKNLKDLIVDAISNRRTMEDILKDFERIPDEESIRKLREISLEALATRHIDLQRILG
                     EQRKAKENRLVPEYVEEFFKRAALLLNIEMEKRSDGLWRIISVPFEIVNQPYDFKTKF
                     GQVQRTYARITFDKEKAFNTQSEFVAMGHPLLEAVVNTILTRYAQSAHDGATFIDPEG
                     KKDGIIWFLDAEIKDGKNEVAGKKLFAIYQNKKNSFSFVNPAVLWDLKPRNVRVSDHS
                     GILLNKDAVVSFVIGEGLENYKRELLDRRLKDAEIKRKYGIRSLEFMILESDSKISDY
                     ETRKMKGENIPEATIQNEVRKKEDLERKKQRLLQEIEAEIHLYPTEPKILAAVRVIPE
                     KTEDEVVSDQEIEQIGMNVAMAYERKNRRMPEDVSFQNLGYDIRSCDENANYRYIEVK
                     TRAKEGALTLTPNEWLMAQRLKHEYWLYIVVNASSNPELYLIQNPAAKLEPDEVIDIA
                     RYIVKDWKKKAQEVR"
     gene            5267..>6542
                     /locus_tag="PLW07_03265"
     CDS             5267..>6542
                     /locus_tag="PLW07_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1156 domain-containing protein"
                     /protein_id="HOL49575.1"
                     /translation="MNVKSFIESDFPVKEVSKESAKEKNIRHGHISTLHIWWARRPLA
                     SSRASIYAALTPEPKDEEERLKRAQFISNLSKWENSLNKDHIDRARKEILEANNNRVP
                     KVLDPFAGGGAIPLEALRLGCQTYAGDLNPVSVLILKCTLEYPQKYGRKKVKDTLLGE
                     KEINPLLEDVKKYGEWVLEEARREISKFYPSEHDGSIPVGYIWARTIKCTNPSCGAEI
                     PLMRQTWLAKKEKKKVAYKLIPGRNRVEFEILEGEKIDFDPEKGTVSRAKVICPCCGS
                     GLSDKEVRKQFQEGKAGQRMVAVVLHHPKRKGKTYRIATEKDLEIFKEAEKYLERKRQ
                     ELMKKWGFDPVPDEGMNTEDPTTIAGRGYGFKKWGDLFNSRQKLALITFVEKVRQAHK
                     KMLEQGYDNEYAKAVVSYLGIAIDRISIYATTL"
BASE COUNT         2282 a         1137 c         1429 g         1694 t
ORIGIN      
        1 tgaagaattc cagaaaaaca gcaacaaaag tttgaaaaac attatgaaaa accttttacc
       61 gcagaacatt attgaagagt ttttgttgat aggaactctt tcaggagaaa tcaaaggaaa
      121 ccaggtgaca gcagaacaaa gaacaattct catttctctt ttgaaaaatt tctgtttcaa
      181 ttttgattcg ttgagaggtt ttgaacaggc aattgtaaca aggggcggag tgagtgtgaa
      241 agaagtaaat ccaaaaacaa tggaatcaaa aatcgtacca ggattatttt tcgcaggcga
      301 aatccttgac atcgacggaa aaacaggcgg gtttaacctg caagccgctt tttcaactgg
      361 ctttgtcgca ggaaaacacg caggttgaaa ccattgagaa gcagttttat aataatatca
      421 tgaccataaa acacattaca accaatcaat cgcatgccga aaaaaatata aaatttcctg
      481 acatcggttt gtctcctact ttttctgaca caggtattgg cgtgtggatt tattgtgatg
      541 attgtatcca ttttatggac aaaatgcttg aaaaatatcc tgatggcatc tttgatatga
      601 tttttgcaga cccgccatat tttctatcaa atggaggcat cacatgtcat gctggcaaaa
      661 tgacaaaggt tgacaaaggc aactgggata aatccagagg ggtcgagcaa aatcatgcat
      721 tcaacatttc ctggctttca agatgccaga aacttttaaa acaaaacgga acgatctggg
      781 tttcagggac tcatcataca atatattcag taggttttgc tatgcaacag ttaggcatga
      841 agatactcaa tgacattagc tgggaaaaac ccaatccacc gccaaatctt tcctgcagat
      901 attttaccca ctcaactgaa acgattatat gggctggaaa gaataccaga tgcagacata
      961 catttaacta tgaacttgta aaacagctgg ctggaggaac acagatgaaa actgtctgga
     1021 gaataccagc gccagcaaag gaagaaaaat tatttggcaa acaccccaca caaaaaccat
     1081 tgaaacttct tgaaagaatt atcattgcga gtaccaacga aggtgacttg atctttgacc
     1141 cgtttcttgg cagcggaact acagcagttg cagcattgaa attaaaaaga aaatttgttg
     1201 gttgtgaact tgaagaatca tttgttgaac ttgctataaa acgggttaca cacgcacttc
     1261 atttaactac caataaactc ccactggatg gttcttaatg aaaaacagtg gtactggcgg
     1321 agaaaaaacc attaccgggc tgaattttga aaagaaggtg gatttacaaa ttttgctttc
     1381 gaaaatgccg ggctatgagt tgaaaaaaat accaggcaaa gccggtactt gtgtattctt
     1441 tgaaggaaaa attgtcgctc gctgtttcag aaaaaacgaa ttttataaat ttttagaaga
     1501 aaacgatatt aaatggcagg cagtgatttc gaagaaattg cttcctgacg atgccctttt
     1561 tgtcatcgta cgggaaaccc tttttatcat tgaggtaaag tatcaacagg tgccagggtc
     1621 agtggacgaa aaacttcaaa catgcgattt taagagaaaa caatacctta aactggttaa
     1681 cccgttagga ctcaaggttg aatatgtgta tgttttgaat gactggttca aaaaacctca
     1741 gtataaggat gtgctggatt acattacgag tgtgaactgt cattacaaat ttcaccaact
     1801 tcctctggca tggctcggac tgccagtgaa acaaagcact gatgtttgaa aatagccaaa
     1861 caaaaatgct aaaatgtgaa tacaggaaat caaggaattc aggttgatga aggttaacag
     1921 ggaaagtacc actgcgatct accgggatag agagaataat gataatgatt gagtccgaca
     1981 atgttcttaa agggccattc tggccagaaa aaattagagt aatttctgta aaacccatcg
     2041 gtagcaacca gataaaaatc gaagccgtag ggcttgagac caatcacttc tataatccag
     2101 tactttctga aaatgatata aaggatatcg aaatccagga aggacgacgt tttctttttt
     2161 caaccgatgg agaatctctt tttctttatc tggaatctca tagaataaga aacgcctttc
     2221 aattcgatcc cctgtacgca atcaatgttt ctcaaattga tccactacct catcagattg
     2281 aagcagtata tcactacata atgcagaacc cccgtatcag atttttgctc gcagatgacc
     2341 ctggtgccgg aaaaaccata atggcagggc ttcttctgaa ggagcttaaa tatcgcgggc
     2401 ttgttgagag aacacttatc attgttccag ggcatctcaa agaccagtgg ctcagggaaa
     2461 tgaaggaaaa gtttcaggaa aacttcttaa tagttgacag gagtattatg aactcagaat
     2521 ggggtacaaa cattttcaaa aatagaaacc aggtcttaat atcaatggat ttcgcaaaac
     2581 aggatgatgt aatttttgct cttaaagatt cgcggtggga cctgtgtatt gttgacgaag
     2641 cccataagat gtctgcctat aaatacggtc agaaaatcag taaaacgcaa agatacaacc
     2701 ttggtgagat tctttcacct cttaccaatt atttaatctt tcttactgca acacctcata
     2761 gaggagaccc tgaaaacttt cgtcttcttc ttgacctttt agaacccggt ttctttgttg
     2821 acacatccat gctttctgaa tccattaaaa ataaagacaa tcccttattt ttgagacgcc
     2881 tgaaagaaga cctgaaggat tttgaaagtg ttcaaatttt tcctcctcgc aatgtggaga
     2941 caacaaagta ctctctctca gacgatgaaa agaagcttta caatgcagtt actgaatatg
     3001 ttgaacagca ctttaacaaa gcccttgaaa aagaaaaacg caatgtcgcc tttgccctta
     3061 caatattgca acgtcgactt gcttcaagtg tccgcgcaat aagaaaatct ttagaaagaa
     3121 gacacaggcg tcttactgaa ttatttcaga agggacagtt gcttagtgac gcagaatacg
     3181 acgagaacta cattgaagat ttagaagaaa aagagcggtg gaagaaagaa gaggaattgc
     3241 tggaaaaact tacttctgct gagacgcttg aggagcttaa tgatgagata ggaaaactcc
     3301 gggagcttgt tcttcttgct aaagaagttg aaaaaaagga gattgagaca aaactgaacc
     3361 agcttaaaaa agtaatggac gacgaaaaac ttcaggaatc aaagacaaaa cttctaattt
     3421 tcacagagtc aaaagatact cttgaatatc tcgttgaaaa acttaatctc tggggctatt
     3481 ctgtggttta cattcatggc gggatgaacc tggacgaaag aatcagggct gaaactgatt
     3541 ttagaaacag ggcgcagata atggtatcaa cagaagccgg tggagagggt ataaaccttc
     3601 agttctgctg gctgatggtg aattatgaca ttccgtggaa tccgacccgt ctggaacaga
     3661 ggatgggaag gatacacagg tatggccagc gacatgaagt tcatatctat aacatggtag
     3721 cagccgatac aagagaaggt cgtattttag aaaaactatt tgaaaaactg tatcaaattc
     3781 gaaggcatct cggttcagac agagtatttg atgttattgg tgaggtattg gttggaaaaa
     3841 atttgaaaga cctgattgtg gatgccattt ctaatcgtag aacaatggag gacattctca
     3901 aagattttga aagaattcct gatgaggaat ctataaggaa attgagggaa atctctcttg
     3961 aagcccttgc gacgagacac attgatttac agcgaattct tggcgagcag cgaaaggcaa
     4021 aagaaaatcg gctcgttcca gagtatgttg aagaattttt caaaagagcc gcgctactgc
     4081 ttaatatcga gatggaaaaa agaagtgacg gactgtggcg tatcatttca gttccttttg
     4141 agattgtcaa ccagccctat gattttaaga caaaatttgg acaggtacaa agaacatatg
     4201 caaggataac ctttgacaag gaaaaggctt tcaatacaca atctgaattt gtagcgatgg
     4261 gccatccact tttagaagcg gtggttaata caattttaac tcgttatgca cagagtgcac
     4321 acgatggtgc cacatttatt gaccctgagg gaaaaaagga tggtattatc tggtttttag
     4381 acgcagagat aaaagacgga aaaaacgaag tagcaggcaa aaaactcttt gcgatttatc
     4441 agaacaaaaa aaattcattc tctttcgtca atcctgctgt tttgtgggac ttaaaaccca
     4501 ggaatgtcag agtatcagac cattcaggaa tcttactgaa taaggacgcc gtcgtgtctt
     4561 ttgtcatagg tgaggggctt gaaaactata agagagaact tttagacagg cggttgaaag
     4621 atgctgagat aaaaagaaag tatggaatcc gttcccttga atttatgatt cttgaatcag
     4681 atagcaagat ttcggattat gaaacaagaa agatgaaagg cgaaaatatc cctgaggcaa
     4741 caattcaaaa tgaagtgcga aaaaaggaag atttagagcg caaaaagcag cgccttttac
     4801 aagaaattga agcagagata cacctatatc ccacagagcc aaaaattctc gcagcagtaa
     4861 gggtaattcc tgagaagaca gaagatgaag tggtcagcga tcaggagatt gagcaaattg
     4921 gaatgaatgt tgcgatggca tatgagagaa aaaacagaag gatgcctgag gatgtttcgt
     4981 ttcagaatct gggctatgat attcgttcat gcgatgaaaa cgcaaactat cgttacatag
     5041 aagtgaaaac gagagcaaag gagggtgcgc tcactctcac gccgaacgaa tggcttatgg
     5101 cacagcgact caaacacgaa tactggctct atattgttgt taatgcctca agcaatcctg
     5161 aactttattt gattcagaat cctgctgcaa aacttgagcc cgatgaggta atagatattg
     5221 caagatacat tgttaaagat tggaagaaaa aagcacagga ggtaagatga atgttaaatc
     5281 tttcatagaa tctgactttc ctgtgaaaga agtgagcaaa gaatctgcaa aagagaaaaa
     5341 cattcgccat ggacacattt ccacgcttca catctggtgg gcaagaagac ctcttgcttc
     5401 ttcaagagca agtatttatg ctgcactcac gcctgaacca aaggacgaag aagagcgatt
     5461 aaaacgggca caattcataa gcaacctttc aaagtgggaa aactccctga acaaagacca
     5521 cattgatagg gcaagaaagg aaattctgga ggcaaacaac aatagggtgc caaaagtcct
     5581 tgacccattt gctggtggcg gtgcaatacc cctggaagct ttgcgattag gatgccagac
     5641 atacgcaggc gatttaaatc ctgttagtgt gcttattttg aaatgtactc ttgagtatcc
     5701 tcagaagtat ggcagaaaga aagtaaagga cactctttta ggtgagaaag aaatcaatcc
     5761 attacttgaa gatgtaaaaa agtacggaga gtgggtttta gaagaagcaa gaagagaaat
     5821 cagtaaattt tatccttctg aacatgatgg ctcaatacct gttggttata tctgggcaag
     5881 aacgataaag tgcacaaacc cctcatgtgg tgctgaaatt ccactaatga gacagacatg
     5941 gcttgcaaag aaggaaaaaa agaaggttgc atataaatta attccaggaa gaaacagagt
     6001 tgagtttgaa attttagaag gagaaaagat tgattttgac cctgagaaag gtacagtttc
     6061 aagggcaaaa gtgatctgtc catgctgtgg ttcgggtttg agcgataagg aagtaagaaa
     6121 acagtttcag gaaggaaaag caggccagag aatggttgca gtagttctgc atcatccaaa
     6181 aaggaaaggt aagacatatc gaattgctac agaaaaagac ctggagattt ttaaggaggc
     6241 agagaaatat cttgaaagga aaaggcagga attaatgaaa aagtggggat ttgaccctgt
     6301 gcctgacgaa ggtatgaata ctgaagaccc aactactatt gccggtcgtg gttatggttt
     6361 taaaaaatgg ggagaccttt ttaattcaag acaaaaactt gccttaatca catttgtaga
     6421 aaaagttaga caggcacata aaaaaatgtt agaacaagga tatgataacg aatatgcaaa
     6481 ggcagtagtg agttatttag gcattgctat tgatagaata tctatttatg ccacaactct
     6541 tg
//
LOCUS       DAORYU010000067         6489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_8294_length_6489_cov_4.331520, whole genome shotgun sequence.
ACCESSION   DAORYU010000067 DAORYU010000000
VERSION     DAORYU010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6489
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8294_length_6489_cov_4.331520"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="PLW07_03270"
     CDS             <1..617
                     /locus_tag="PLW07_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49576.1"
                     /translation="LPNQQEIGGRSGEGRTGRSYGEMVEKTAQGKGGRQTPARLTPDN
                     IEPGQVQDTSGENQLGPTGGGKTSGWGPAGLKGPVQDLSFRYTELAEKQAKLIERAEK
                     IERELKILNIYNAQLEQSISAMKQFTIHLKEGRYNNLLTTNQMIVNNLKQAQQVLTNQ
                     AIIRVENSERIEKKRKELGSIWDEKIPSGYEPVVRKYYENIAGR"
     gene            595..1536
                     /locus_tag="PLW07_03275"
     CDS             595..1536
                     /locus_tag="PLW07_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49577.1"
                     /translation="MKILLAGNVFLLFICSVWGMQLVLESSKDTYQEEETVLLNLTII
                     NDSTQIESHIVEFLQPREKNWMYIGNIHKLVIKNIPAMEHFTNHMPPEPKPHSPQTNK
                     NYKPVVLNPGESYRILIPFFYEYYPVKLPATFNVQLHWLDTVSNPVSFTVLATKGTKK
                     QENIIINGDFSEGEGKIPYGWKILDDRTLWDSTEKFLMYNVDRPTAESEGYWAYSIFH
                     EIPSPSDYVLSVRTKTNGPIIIVFVEGWGMVEGRRRRIERNECFYHQAPDQTWKTQIR
                     NVTFKNPAVKWVRLKLYVYGNPGKVWFSDLLMIPVKK"
     gene            1533..3554
                     /locus_tag="PLW07_03280"
     CDS             1533..3554
                     /locus_tag="PLW07_03280"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOL49578.1"
                     /translation="MKKNILFVSLWFLFWFSQCGFGDIWLDGYPYRAEVLVPEARDNI
                     PVEQAAIVRIFCPATDDGSDITITDENGKLIDFFVSQTGPGNFYEICFPFSGKKYYLF
                     YGKKDTGFHGLNYKPKRGILLELYAMRGDNVDTWESCKKIIDDSVQKKYFVNAAFRKT
                     IYDATNPITKTGHFVRVYTGYFYLEKKEKISFGTTSTGASFILVDDKPVASWPGRHWI
                     EGFIRPEHAGDIELESGIHKLVYYHFEFPGRGYAIAAMKKPSDKQFVPIDEKFFLPIL
                     EAKITGIEKKNSELCASFFWQNTNYLYRENWQLVTFRFTDASRGKNDIVSWQWEFGDG
                     QTSNEQHPIHTYFLQKIYTVTLTITDKKGNSDTVSMNVRAEQDYSISTLYPRSFSEYM
                     NEFNSYNLSKFPVEELFALAEICNSYDNKELAFNCYNELLNRNLEINQHINVALTAAE
                     IATQLKKYSDAEKIYKHLIESNNIPEAKVKLGFVYLASNKIDAAEQQFNLVLSDKNTG
                     EKIKRAATIGLGDTARYRADTKTALKCYESALSDTSIERKSGIYSQQVLFYLKKKDVS
                     TALEKLSQWADEIPTCKLKGDWSILSARTYLLKKDYDSAFRELETYQRICDNPESPYS
                     GWATYLKGEIYLEKGEKEKAKEHFLKVVENFPQSQISDMAKEKLKEMEK"
     gene            3551..5308
                     /locus_tag="PLW07_03285"
     CDS             3551..5308
                     /locus_tag="PLW07_03285"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOL49579.1"
                     /translation="MKEKIKIETHNADICVVGGGMAGICAAISAARNGSRVVLMHDRP
                     VLGGNASSECRVHICGADRHSRIKNLRETGILEELRLENLTRNPNRSYSIWDLVLFEK
                     IKAEQNITLVLNCSCFDTEMDGNFIVSISGWQTTTQVIHKVRAKIFIDCSGDAIVAPL
                     TGALYRMGREAKNEFNESYAPDIADSKTMGMSCMFMAKEYDTPQKFQPPSYAYQFDTC
                     DQLPYGKQGHSWFEMGYWWIELGGEYHSIYDTEKLRDELLKIVLGVWDHIKNRCPDKH
                     KAKNWALEWLQFLPAKRESRRYIGEHVLTQNDIESEGKFDDIVAYGGWPMDDHHPAGF
                     FSVRVNAPSTIFHPSPSPYGIPYRCLYSKNITNLMFAGRNASCTHIAMSSTRVMGTGC
                     SMGQAAGTAAAIAIKKNIMPADVLKYVDLVQQTLLYDDAYIPYIKQQMPELTHLANID
                     ASKGNPEPVRDGINRPVGKQSNGWKCQENDWISYTFTEETFINKITLIVDSGLDKLIA
                     MSHLQKDNQLTSLPDTMPAQFRIDVKQNGRWKTLLKIKNNNQRLFRCEIKQKVTGIKF
                     VLEKTYGVTTSCVYAFYCE"
     gene            complement(5314..>6489)
                     /locus_tag="PLW07_03290"
     CDS             complement(5314..>6489)
                     /locus_tag="PLW07_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOL49580.1"
                     /translation="IGTSVQGRKILAVRITGLSDSLFKPKVFLTSTIHGNELTGYAVL
                     LYLIDYLVSEYGRNPVVTEILDRCEIWIHPLANPDGTYWAGDDNVFGARRYNANSIDL
                     NRNFPDPAEGNHPDGNEYQSETISMMNVASTEKFVLSANFHSGAELVNYPWDTWEKLH
                     PDNNWFVFISRQYADTVHLYSPDTYFDDLDNGITNGYAWYRITGGRQDYMNYFKHCRE
                     VTIELSEEFLPFNPSSYWEYHKDALIGLIAQSLTGICGTILCETGIPLPAKIEIPGHD
                     ADNSFVLSETQTGRFFRLLLPGNYNLRFSADYHYPQEIENIQVSKDNLTQINVVLKHA
                     EKADPSADHMIDIVDVILCLKMAIGLQQIDLASCDMNDDGFIDISDVILMLRASIGL"
BASE COUNT         2229 a         1232 c         1276 g         1752 t
ORIGIN      
        1 tattaccaaa tcaacaggaa attggtggaa gaagcggcga aggaagaaca ggtcgctcct
       61 atggtgagat ggttgaaaaa accgcccagg gaaaaggcgg caggcaaacg ccagcaaggt
      121 tgacccctga taatattgaa ccaggacagg ttcaggacac cagtggcgaa aatcaattag
      181 gaccaacagg tggaggaaaa acaagtggct ggggtccagc aggacttaag ggacctgttc
      241 aggatttatc gttcaggtac actgaactgg cagaaaaaca ggcaaaactg atagaaagag
      301 cagaaaaaat tgaaagagaa ctgaaaatac tgaacattta caatgcgcaa cttgaacaat
      361 caatttctgc aatgaaacag tttaccatac acttgaaaga aggcagatat aataaccttc
      421 tcacaacaaa tcagatgatt gtgaacaatc tcaaacaggc gcaacaggta ctcacaaacc
      481 aggcaattat aagggtagaa aattctgaaa gaattgagaa aaaaagaaaa gaactcggaa
      541 gtatctggga cgagaaaatt ccgtcaggat acgaacctgt cgtgaggaaa tattatgaaa
      601 atattgctgg caggtaatgt gtttttatta ttcatttgtt ctgtgtgggg aatgcaactt
      661 gtccttgaaa gctcaaaaga tacctatcag gaagaagaaa ctgtacttct gaatcttacc
      721 atcataaatg actcaacaca gattgaaagt catatcgtcg aatttctcca gccaagagaa
      781 aaaaactgga tgtacatcgg caatattcat aaacttgtca ttaagaacat tcctgcaatg
      841 gaacacttta ccaatcacat gccgccagaa ccaaaacccc acagtccaca gacaaataaa
      901 aattataaac ctgttgtcct caaccctgga gaatcttata gaattttgat tccatttttt
      961 tatgaatatt atccggtgaa attgccagcc acattcaatg ttcaactgca ctggcttgat
     1021 acggtttcaa atccagtcag tttcacagtt cttgcaacaa agggaacgaa aaaacaggaa
     1081 aacattatta tcaatggaga tttttcagag ggcgaaggaa aaatccctta tggatggaaa
     1141 attcttgatg atagaacact ctgggattca acagaaaaat ttttgatgta caatgttgat
     1201 agacccactg ctgaaagtga aggatactgg gcatattcaa tttttcatga aattccttcg
     1261 ccttctgatt atgttttaag cgtcagaaca aaaacaaacg gacccataat cattgttttt
     1321 gttgaaggat ggggaatggt tgaaggaaga agacgtagaa ttgaaagaaa cgagtgtttt
     1381 tatcatcaag cgccagacca gacctggaaa actcagataa gaaatgttac gttcaaaaat
     1441 cctgcagtca aatgggtacg gttaaaactt tatgtttatg gaaatcctgg aaaggtgtgg
     1501 ttttctgatt tattaatgat tcctgtgaaa aaatgaaaaa aaacatttta tttgtgtctt
     1561 tgtggttttt attctggttt tcccaatgcg gtttcggaga catatggctt gatggatatc
     1621 catatcgtgc tgaagtactt gtccctgaag caagagacaa tatccctgtg gaacaggcag
     1681 ccattgttag aatattctgt ccggcaacgg atgatggctc tgacatcaca atcacagacg
     1741 aaaatggaaa actgatagat ttttttgttt cgcaaacagg acccggtaat ttttatgaaa
     1801 tctgttttcc tttttcagga aaaaagtact atcttttcta cgggaaaaaa gatactggtt
     1861 ttcatgggtt aaactataaa ccaaaacggg gtattttact tgaattatac gcaatgcgtg
     1921 gtgacaatgt tgatacatgg gaaagttgta agaaaatcat tgatgacagt gttcaaaaaa
     1981 agtatttcgt caatgctgca ttcagaaaaa caatttatga tgcaaccaat cccattacaa
     2041 agacaggtca ttttgtcaga gtgtacactg gatatttcta tcttgaaaaa aaggaaaaaa
     2101 tttcatttgg aacaacatcc actggcgcat catttatcct tgttgacgat aaacctgttg
     2161 catcctggcc tggcagacac tggatagaag gatttatcag accagaacac gctggagata
     2221 ttgaacttga gtcaggtatt cacaaacttg tgtactatca ttttgagttt cctggccggg
     2281 gttatgcaat tgctgcaatg aaaaagccct cagataaaca atttgtccca atagatgaaa
     2341 aattttttct cccgattctt gaagcaaaaa tcacaggcat tgagaagaaa aattctgaat
     2401 tatgcgcatc atttttctgg cagaatacca attatcttta cagggaaaac tggcaacttg
     2461 tcacattcag gttcactgac gcttccagag gaaaaaacga cattgtatcc tggcaatggg
     2521 agtttggaga tggacagacc agcaatgaac aacatccgat acacacatat tttttgcaaa
     2581 aaatttacac tgtaactctc acaatcactg ataagaaagg taattctgac actgtatcaa
     2641 tgaatgtaag agcagaacag gactacagta tttcaaccct gtatccacgt tctttcagtg
     2701 aatacatgaa tgaattcaat tcatataacc tgtcaaaatt tcctgttgaa gaactctttg
     2761 cccttgctga aatctgtaat agttacgata acaaagaact tgcatttaac tgttacaatg
     2821 aactgctaaa tagaaatctt gaaataaacc agcatatcaa tgttgctctt actgccgctg
     2881 agattgccac acaattaaaa aagtattctg acgctgaaaa aatttacaag catctcatag
     2941 aatcaaacaa tattcctgaa gcaaaggtca aacttggctt tgtgtatctt gcatcaaata
     3001 agatagatgc tgctgaacag cagttcaacc ttgttctttc agataaaaac acaggcgaaa
     3061 aaataaaaag ggctgccaca atcggactgg gtgataccgc aagatacaga gcagacacaa
     3121 aaaccgcact gaagtgttat gaaagcgctc tttcagatac atccattgaa agaaagtcag
     3181 gtatttattc acagcaggta cttttttacc tgaaaaagaa ggatgtttcc actgctttag
     3241 aaaaactttc acagtgggca gacgaaatac caacctgcaa acttaaagga gattggtcta
     3301 tcctatcagc aagaacatat ttattgaaaa aggattacga tagtgccttc agagaacttg
     3361 aaacatatca gagaatatgt gataaccccg aaagtcccta ttcaggatgg gcaacctatc
     3421 ttaaaggtga aatatactta gaaaaagggg aaaaggaaaa ggcaaaagaa cattttctca
     3481 aggtggtcga aaacttccct caatctcaaa tatcagacat ggcaaaggaa aaactcaagg
     3541 agatggaaaa atgaaagaaa aaataaagat tgaaacacat aatgctgaca tatgtgttgt
     3601 tggcggtggg atggctggaa tatgcgctgc gatatctgct gcaagaaacg gaagtcgcgt
     3661 agttctgatg catgacagac ctgtgcttgg tggcaacgcg tccagtgaat gccgcgttca
     3721 catctgtgga gcagacagac actcacgaat aaaaaatttg agggaaaccg gtatccttga
     3781 agaactgcgg cttgaaaatc tcactcgcaa tccaaacaga agttattcaa tctgggacct
     3841 tgttctcttt gaaaaaatca aagcagaaca aaatatcacg cttgttctca attgctcgtg
     3901 ttttgataca gaaatggatg gaaattttat tgtttctatt tctggatggc aaacaacaac
     3961 tcaggttatt cataaagtgc gcgcaaaaat ctttatagat tgttcaggtg acgctattgt
     4021 tgctccactt actggcgcac tttacagaat gggaagggaa gcaaaaaatg aattcaatga
     4081 atcatatgcg cctgacatag cagatagcaa gaccatggga atgagttgta tgtttatggc
     4141 aaaagaatac gatacacctc aaaaatttca acctccttca tacgcgtatc aatttgacac
     4201 atgtgaccag ttaccttacg ggaagcaagg gcactcctgg tttgaaatgg ggtactggtg
     4261 gattgaactt ggcggcgaat atcattccat ctacgatact gagaaactgc gcgatgaact
     4321 attgaaaatt gttctcggag tgtgggacca cataaaaaac cgatgtcctg ataagcataa
     4381 ggcaaaaaac tgggcgcttg aatggttgca gtttttacct gcaaaaagag aaagcagacg
     4441 atatattggt gagcatgtct taacacaaaa tgacattgaa tcagaaggaa agtttgatga
     4501 cattgttgcc tacggcggct ggcccatgga tgaccatcat cctgcaggat ttttttcagt
     4561 cagggtcaac gcaccatcca cgatttttca tccttcacct tcaccatatg gcataccata
     4621 tcgttgcctt tattcaaaaa acataacaaa tttaatgttt gctggaagaa atgcaagttg
     4681 tacccacatt gcaatgagtt ccacccgtgt aatgggaact ggttgcagta tgggccaggc
     4741 agcaggaaca gcagcagcca tcgcaataaa aaagaacata atgcctgcag atgttctgaa
     4801 atatgttgac cttgttcaac aaacacttct gtatgatgac gcgtatatcc cttacattaa
     4861 gcaacagatg cctgaactta cacatttagc aaacattgac gcatcaaaag gaaatccaga
     4921 accagtaagg gatggcatca acaggccagt tggtaagcag tcaaatggat ggaaatgcca
     4981 ggaaaatgat tggatatcgt acacgttcac agaagaaaca ttcataaaca aaataacatt
     5041 aattgttgat agcggactgg acaaacttat tgcaatgagc catctacaaa aggataatca
     5101 actcacatcg ttacctgata caatgccagc acagttcaga attgatgtca aacaaaatgg
     5161 gagatggaaa acattgctaa aaatcaaaaa caataaccaa cgacttttca gatgcgaaat
     5221 aaaacagaaa gtcacaggta taaaatttgt tcttgaaaaa acttacggag tgacaacatc
     5281 ctgtgtgtat gccttttact gcgaatgatt tctttataaa ccaatgctgg ctcttaacat
     5341 caatatcaca tcgctgatgt caatgaaacc atcatcattc atatcgcaac ttgcaaggtc
     5401 aatctgctga agacctattg ccatttttaa acacaaaatc acatcaacaa tgtcaatcat
     5461 atggtctgct gatgggtctg ccttttccgc gtgtttcagt acaacattta tttgtgtcag
     5521 attatccttt gatacctgaa tattttcaat ctcctgcggg tagtgataat ctgcagaaaa
     5581 acgaagattg taatttcctg gtagaagcaa cctgaaaaat ctacctgttt gtgtttcaga
     5641 caaaacaaat gagttgtctg catcatgtcc tggaatctca atttttgcag gtaaaggtat
     5701 cccagtttca cataaaatag ttccacatat gcctgtcaaa ctctgtgcaa tcaatccaat
     5761 gagggcatct ttatgatact cccagtatga agaaggatta aaaggcaaaa attcctcact
     5821 caattcaatc gttacttccc tgcaatgctt aaaataattc atataatcct gtcttccacc
     5881 agtaattctg taccaggcat agccattggt aataccattg tcaaggtcat caaaatatgt
     5941 gtcaggtgaa tatagatgaa cggtgtcagc atactgtctg ctgataaaaa caaaccagtt
     6001 attatcagga tgcaatttct cccatgtatc ccagggataa ttaacaagtt cagcaccaga
     6061 gtgaaaattc gcagatagca caaatttttc agttgatgcg acattcatca ttgagattgt
     6121 ttctgattga tactcattac catcaggatg attgccttct gcagggtctg gaaagttgcg
     6181 gttcagatca atgctatttg cattgtacct ccttgccccg aaaacattat cgtctccagc
     6241 ccagtaagta ccatcaggat ttgccagtgg atgaatccat atttcgcatc tatcaagaat
     6301 ttctgtaaca acaggatttc tcccatattc tgacacaaga taatcaatga gatataaaag
     6361 taccgcgtaa ccagttaatt cattcccatg gattgttgat gtcaggaaaa cttttggctt
     6421 gaacaatgaa tcagataaac ctgtgattct cacagcaagg atttttcttc cctgaacact
     6481 tgttcctat
//
LOCUS       DAORYU010000068         6331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_8522_length_6331_cov_4.273263, whole genome shotgun sequence.
ACCESSION   DAORYU010000068 DAORYU010000000
VERSION     DAORYU010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6331
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8522_length_6331_cov_4.273263"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            351..2801
                     /gene="leuS"
                     /locus_tag="PLW07_03295"
     CDS             351..2801
                     /gene="leuS"
                     /locus_tag="PLW07_03295"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOL49581.1"
                     /translation="MKKYEPSSIEEKWRKIWEETSVNYTDVSNVTVKKYYCLDMFPYP
                     SGSGLHVGHWRGYVLSDVWARYKRLQGYHVLHPMGWDSFGLPAENDAIKKGIHPRIST
                     KNNIDNIRRQLKEIGAMYDWTKEINTSDPEYYRWTQWIFLQMYHRGLAYRAEMPINWC
                     PNCKTGLANEEVISGNCERCGSTVTRKRLMQWMLKITAYAERLLADLDRLDWPEKVKT
                     MQKNWIGRSEGASIIFMINGNKNIKLEVFTTRPDTLFGATYVVLAPEHPLTLEIASSE
                     KISEVRKYVKEATVKSDLERTQLTREKTGVFTGCYAINPVNGKKVPVWVADYVLAHYG
                     SGAIMSVPAHDQRDFEFAKKFGLPVIEVIRGDMAERNVDGSLSVAYEGPGVLINSGQF
                     TGMNSEEAKHAITRWLEEKGTGSFKVTYKLRDWVFSRQRYWGEPIPIIYCKRCGTVPV
                     DEKDLPVLLPDVESYKPTGTGKSPLAAIEKFVHTTCPKCGGPADRETDTMPQWAGSSW
                     YFLRYPEPALKTAPFDRKVVDRWLPVDCYIGGVEHAILHLLYARFFTKFLYDIGTIGF
                     DEPFLKLFNQGMVTKFSEKTGKLEKMSKSKGNVVNPDPLVKKYGTDTVRLYELFIGPP
                     ELDSEWNDAGIEGCYRFLKRAWDFVISQVEKNCITDDVSKKQLHVLVKKVTERIEDFK
                     LNTAISAFMEFMKDVPENACYSKEDIAKFIVLLSPFVPHFSEELWREILGYPDTVFNA
                     TWPEFDSSILSEETITIPVQVDGRVRGTISIDKRATAEKVFEMVLKNQSISRFISGRN
                     VIKKIYIPEKIVNLVTEE"
     gene            complement(2852..3088)
                     /locus_tag="PLW07_03300"
     CDS             complement(2852..3088)
                     /locus_tag="PLW07_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49582.1"
                     /translation="MGALIGGIIAVIVGLIGIVKWWTLFVKGLQAAVPFFILLVGVIA
                     IAAGISDIKDRIEEKKEKEKTKQEAEKPQQSEQK"
     gene            complement(3159..4010)
                     /locus_tag="PLW07_03305"
     CDS             complement(3159..4010)
                     /locus_tag="PLW07_03305"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOL49583.1"
                     /translation="MAEKFLIFLSVEKNLSQNTLQAYRQDMKNFFRFLTINHISYRYI
                     KAKNLLDFMIFLKRKNFASATIARNISSIRGLYRYLVSKGDISSSILNLFESPKIERK
                     IPSIIGKQDVDRIISTKISKRDKVNKRNLAILGLLVTTGLRISELAGLKKTDINLREN
                     WIRVTGKGKKERIVFFPQELVPFVNHCMTGPGEFLFGTKTGKPISRQNIWKIVHKTGK
                     TAGLQINLKPHMLRHTFATQLLEAGMDIRVIQELLGHKSISTTKIYTLVSRNQLKTIH
                     KKFHPRA"
     gene            complement(4039..4686)
                     /locus_tag="PLW07_03310"
     CDS             complement(4039..4686)
                     /locus_tag="PLW07_03310"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOL49584.1"
                     /translation="MNLINSEVMIEKLTVGQMATNCYIVSCDDKSATIIDPGSDADLI
                     STHIKKRKLNVRYIINTHGHYDHIGANHIKHMIGNNVLLGIHKSDEEYLKTPELNLST
                     LVGIDYVSDSPDFFLQENQVIHLTKKIFFSVIHTPGHTPGSVCLKLENYLFSGDLLFC
                     GGVGRTDLPGGNENLLSKSLHTILELDKNILVLPGHGPETTIGNEIETNPFLVFI"
     gene            complement(4690..>6331)
                     /gene="ligA"
                     /locus_tag="PLW07_03315"
     CDS             complement(4690..>6331)
                     /gene="ligA"
                     /locus_tag="PLW07_03315"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOL49585.1"
                     /translation="DGVAVSLTYENKTLVRGATRGDGFLGDDITQNIKTIRDIPLTVV
                     NDEKFEVRGEVYMPKFDFEKLNREKLETGEEPFANPRNAAAGSLKLLDPKEVAKRHLR
                     FFVYAGFFQKTNPLTQEETLNILKNLGFPVNPHRKLARTIDEVIEFCREWESKRNQID
                     YAIDGIVIKVNSIRDQEILGSTSKSPRWMVAYKFPAEQATTTIKDVIVQVGRTGTLTP
                     VAMLEPVRLAGTTVSRASLHNFDEIKRLDVKIGDRVFVEKAGEIIPKVVKVIKEARSG
                     NEHIINPPEKCPVCHNPVVKDENEVAYRCPNVSCPAQVKERILHFASRRAMNIQGLGE
                     RIVNELVDTGLVKDYADLYFLQPQQIEQIERMGEISSKKLADNIQASKNAPFARFIYG
                     LGIRHIGERASEILAENFTDIDEIAHASEDKIAQIPDIGQVAAHSIKEFFNNPANITL
                     IEKLKQIGVSMKQSQILKRPSKISGKKFVITGTLKSFSREEMKTELKKHGARVSDNIS
                     KETDFLIVGENPGSKLEKAKKLGITIVTEEEALQMMKE"
BASE COUNT         1849 a         1144 c         1221 g         2117 t
ORIGIN      
        1 tcatctttag gatatatgac accagtggaa tttgaacaaa aagagataaa acaattattt
       61 ttaaccagga cgccttttgc ccttgacaaa tgggagcagt gcagaaattc tatagatatt
      121 gttaaactca caaaaacaat attcgcactt aaagaaaaat tattctagaa ttaccaaaaa
      181 aaaggagaaa agaaaaatgc cttatagaaa tataatttca aatagatcta tttttgagga
      241 aatacggagt atgaatttta acatgaattg aaaaatatca aatcttgatg tatagttaat
      301 tattatgcaa cttttgtttg ccagaccaag cgcattttat cgggagtaac atgaaaaaat
      361 atgaaccgtc ttcaatagaa gaaaaatggc gaaaaatatg ggaagagaca agtgttaatt
      421 atacagatgt cagtaatgta acagtaaaaa aatattattg tctcgatatg ttcccatatc
      481 cttcagggtc agggttacat gttgggcact ggagggggta cgttctttcc gatgtgtggg
      541 ctcgatataa aagacttcag ggatatcatg ttctgcatcc aatgggctgg gattcttttg
      601 gtctgcctgc agaaaatgat gcaataaaaa agggtataca cccaaggata agtacgaaaa
      661 acaatataga taatatacgc agacaattaa aagaaatagg tgcaatgtat gattggacca
      721 aggaaatcaa taccagtgat cctgagtatt atcgctggac acagtggatt tttctccaga
      781 tgtatcatag aggacttgca tatcgagcag aaatgccaat caactggtgc cctaattgca
      841 agacaggtct ggcaaatgaa gaagttatca gtggcaattg cgaaagatgt ggaagtacag
      901 taacaagaaa aaggttaatg cagtggatgc ttaaaatcac tgcatatgca gaaagattac
      961 ttgctgacct tgatcgtctg gattggcccg agaaagtaaa aacaatgcaa aagaactgga
     1021 taggtagaag tgaaggagca agtatcattt ttatgataaa tggtaataaa aacattaaac
     1081 ttgaagtttt caccacaaga cccgacactc tttttggcgc tacttatgtc gtgcttgcac
     1141 cagaacatcc gttgacactt gagattgctt catcagaaaa aatatcagaa gttagaaaat
     1201 atgtcaaaga agccactgta aaatcagacc ttgaaagaac ccagttgact cgtgaaaaaa
     1261 caggagtttt taccggttgt tatgccatta atcctgtcaa tgggaaaaaa gtcccagtgt
     1321 gggtggctga ctatgtgctt gctcattacg gttctggtgc aattatgtca gtaccggctc
     1381 atgatcagag agattttgaa tttgcgaaaa aatttggatt gccggtaatt gaagtgattc
     1441 gtggtgacat ggccgaaaga aatgttgatg gttcactttc tgttgcttat gagggtcctg
     1501 gagtattgat aaactccggg caatttacag ggatgaattc agaggaagcg aaacacgcaa
     1561 ttactagatg gcttgaagaa aaagggacag gttctttcaa ggttacctat aaattgagag
     1621 actgggtatt ttcccgacag agatactggg gagagccaat accaatcata tattgtaaga
     1681 gatgcggtac agtgcctgtg gatgaaaagg atttgcctgt tcttcttcct gatgttgagt
     1741 cctataaacc aacgggaacc gggaagtccc ctcttgcagc gattgagaag tttgttcata
     1801 ctacctgtcc aaaatgtggt ggacctgctg atcgagaaac agatacaatg cctcaatggg
     1861 ctggttcttc atggtatttt ttgagatatc ctgaaccggc tttgaaaact gcaccttttg
     1921 accgtaaggt tgtggacagg tggttgcctg tagattgtta tattggtggc gttgaacatg
     1981 ctattcttca tttgctttat gccagatttt ttacgaagtt tctttacgat attggtacga
     2041 tcggatttga tgagccattt ttgaaacttt tcaatcaagg aatggtgaca aaattcagcg
     2101 agaaaacagg gaaacttgag aagatgtcaa aatccaaggg gaatgttgta aatcctgacc
     2161 ctcttgtaaa gaaatatggt acggacacag tgaggcttta cgaattgttt attggtccgc
     2221 ctgaattgga ttctgaatgg aatgacgctg gaatagaggg ttgttacaga tttttgaaaa
     2281 gggcctggga ttttgtgata tcacaggttg agaaaaactg tataactgac gatgtttcaa
     2341 aaaaacagtt acatgttctt gttaaaaagg tcacagaaag aatagaggac ttcaaactca
     2401 atacggctat tagtgccttt atggaattta tgaaagatgt acccgaaaat gcctgttatt
     2461 caaaagaaga cattgcaaaa tttattgttc ttctttctcc gtttgttcct catttttctg
     2521 aagaattgtg gcgggaaatt ctgggatatc ctgatactgt tttcaacgcg acctggcctg
     2581 aatttgatag ttctattctt tcagaagaga caataacaat tcctgtgcag gttgatggca
     2641 gagtccgggg gacaatttca attgataaac gtgcgactgc tgaaaaagtt tttgaaatgg
     2701 tattaaagaa ccagagtatt tcaaggttta taagtggcag aaatgtcatc aagaaaatct
     2761 acatacctga aaagattgtt aaccttgtaa ctgaggagta aaaaatagga gccggctttt
     2821 gaaccggctc cttatctggt ttaaaattgt tttacttctg ttcactctgc tgcggttttt
     2881 ctgcttcctg ctttgttttt tctttctcct ttttttcttc tattctatct ttaatatcgc
     2941 tgatacctgc tgcgatcgct ataaccccga caagcagaat aaaaaatgga acagctgcct
     3001 gaagtccttt aacaaacaat gtccaccatt ttacaatacc tattaaccca acgattaccg
     3061 ctatgatacc gcctattaat gcgcccattc atcctccttt tttgcgtcgt gttcacctgt
     3121 ctcatttatt attacatgat attttatgca tgtcaattct acgctcttgg atgaaatttt
     3181 ttatgtatgg tttttaactg atttcttgat accagggtat aaatttttgt ggtactgata
     3241 cttttatgac caagtaactc ttgaatcact ctgatatcca ttcctgcctc aaggagttgt
     3301 gtggcaaatg tatgtctgag catatgaggt ttaagattaa tctgaagtcc tgcggtttta
     3361 cctgttttat gcacaatttt ccagatattc tggcggctta ttggttttcc agtttttgta
     3421 ccaaacaaaa attctcctgg tccggtcata caatgattaa caaaaggaac aagttcctga
     3481 ggaaagaaaa caattctttc ttttttccct tttccagtga cccgtatcca gttttctcta
     3541 agattaatgt ctgttttctt taacccagca agttctgaga tacgcaaacc agtggtaaca
     3601 agcaagccaa gaattgctaa atttctttta tttaccttat ctcttttaga aattttcgta
     3661 gaaatgattc tatccacgtc ctgttttcct attatggaag gtatctttct ttcgattttc
     3721 gggctttcaa acagattaag gatagaagaa ctgatgtcac ctttacttac caggtatcga
     3781 taaagtcctc tgatggaaga aatgtttctt gctatggttg ctgatgcaaa attttttctt
     3841 ttgagaaaga tcataaaatc aagaagattt tttgccttga tgtatctata agaaatatga
     3901 ttaattgtca aaaatctaaa aaaatttttc atgtcctgtc tgtatgcctg taaggtattt
     3961 tgcgatagat tcttttctac agacagaaaa attaaaaatt tttcggccat ttcatgaagt
     4021 tgtgtgtgtt ccatattgtt atatgaaaac cagaaacgga tttgtttcta tttcgttgcc
     4081 aattgttgtt tcaggtccat gaccgggtag taccaagatg tttttatcaa gttccaaaat
     4141 tgtatgtaat gatttcgata acaaattttc atttcctcct ggtaggtcgg ttcttcccac
     4201 gcctccacaa aatagcaggt ctcctgaaaa aaggtagttt tcaagtttta agcacacact
     4261 tccaggtgtg tgtccggggg tatgaatcac agagaaaaaa attttcttcg ttaaatgaat
     4321 tacttgattc tcctgaagaa aaaaatcagg agaatctgaa acataatcaa tgcccaccaa
     4381 tgttgaaagg ttgagttcag gggttttcaa atattcttca tcgcttttat gtatcccgag
     4441 caaaacatta tttcctatca tatgttttat gtgatttgcc cctatgtggt catagtgtcc
     4501 gtgtgtattg atgatgtatc tgacgttaag ttttcttttt ttgatatggg ttgagatcag
     4561 atctgcgtcg gaacctggat caatgatcgt tgcgctttta tcatcgcagg atacaatgta
     4621 gcagtttgtt gccatctgtc caacagtgag tttttcaatc ataacttcag aatttattaa
     4681 gttcataatt tactccttca tcatttgaag ggcttcttct tctgtaacga ttgtgattcc
     4741 gagttttttt gctttttcaa gttttgaccc cggattttcc cctacaatga gaaaatctgt
     4801 ttcttttgaa atgttgtctg aaactcttgc tccatgtttt tttaattctg ttttcatttc
     4861 ttctcttgaa aagcttttaa gagttccggt aattacaaat tttttcccag aaattttact
     4921 tggtcttttt aaaatttgag actgcttcat agaaacacca atctgtttaa gtttttcaat
     4981 aagtgtaata ttggcaggat tattaaaaaa ttctttaata ctatgcgcgg caacctgtcc
     5041 aatatctgga atttgtgcaa tcttgtcctc agaggcgtgc gcaatttcat caatatctgt
     5101 gaaattttca gcaaggatct cgcttgccct ttctccaata tgtctgattc caagaccgta
     5161 gataaatctg gcaaaaggtg catttttgct tgcctggata ttatccgcaa gtttcttgct
     5221 tgatatttct cccattcttt ctatttgctc aatttgctgg ggttggagaa aataaaggtc
     5281 tgcataatct ttaacaagac cggtgtcaac aagttcattg acgattcttt ctccaagacc
     5341 ctgaatattc atagccctgc gacttgcaaa gtgaagtatc ctttccttaa cttgagcggg
     5401 acagctaaca ttcggacatc tatatgcaac ttcgttttca tctttgacca caggattatg
     5461 acatacagga catttttcag gtggattgat gatgtgttca tttcctgacc gtgcttcttt
     5521 gatgacttta acgacttttg gaataatttc acctgccttt tccacgaaaa cacggtcccc
     5581 aatttttacg tcaagtcttt ttatttcatc aaaattatgt aaactggctc tgcttaccgt
     5641 tgttcctgct aaacgaactg gttcaagcat tgccacaggt gttaaagttc ctgttcttcc
     5701 aacctgaact ataacatcct taattgttgt tgttgcctgt tcagctggaa atttgtaagc
     5761 caccatccat ctcggacttt ttgatgtgct tccaaggatt tcctgatctc tgatactgtt
     5821 gacttttatc acgataccgt caatagcata atctatctga tttctttttg attcccactc
     5881 gcgacagaat tctattactt catcaatcgt ccttgctaat tttctgtgag gattaaccgg
     5941 aaatccaaga tttttcaaaa tattcagtgt ttcttcctga gtcaaagggt ttgttttctg
     6001 aaaaaaccca gcatacacaa agaatctcag atgtcttttt gccacttctt ttgggtccag
     6061 cagctttaat gaacctgcgg ctgcatttct tggatttgca aatggttctt caccagtttc
     6121 caacttctct ctgttcagtt tttcaaaatc aaattttggc atgtagacct cgcctcttac
     6181 ttcaaatttt tcatcattga cgacggttag aggtatatct cttatggttt tgatattttg
     6241 tgttatatcg tctccaagaa atccgtcgcc ccttgtcgca cccctgacaa gcgttttatt
     6301 ttcatatgtt aaagatactg ctaccccatc a
//
LOCUS       DAORYU010000069         6289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_8585_length_6289_cov_4.239654, whole genome shotgun sequence.
ACCESSION   DAORYU010000069 DAORYU010000000
VERSION     DAORYU010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6289
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8585_length_6289_cov_4.239654"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..130
                     /locus_tag="PLW07_03320"
     CDS             <1..130
                     /locus_tag="PLW07_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOL49586.1"
                     /translation="GPIIKALKEISYQGYISVEVFDFSPGPENIAEKSIQYLKKFL"
     gene            complement(135..524)
                     /locus_tag="PLW07_03325"
     CDS             complement(135..524)
                     /locus_tag="PLW07_03325"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOL49587.1"
                     /translation="MAAITVKLANRLKFEAQIRQHCIAIDLPAELQGTDTGPMPPEFF
                     VLSLASCMGYYALFYCTKNRIPAEGLKIDADFEKTKNPDRISTIEVKISIPGLPEEHK
                     KGVMEAVNNCIVKQSILIKPEINVSIG"
     gene            614..1396
                     /locus_tag="PLW07_03330"
     CDS             614..1396
                     /locus_tag="PLW07_03330"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOL49588.1"
                     /translation="MIKIDVCIDTVFPELKDEEKIEKVKEIGYQCVEFWFHDNKNVEA
                     IAKALKNSQVKLNNLVVNSPDGSRGGSLVKKEDQQTYLERLKYVISIAKSLDCNMGIT
                     CTGNSIPDLSKSQMMDNIIETLSMACDILEKECFTLVLEPLNTLVNHKGYFLDSATDA
                     AEIIRKVNNQRVKLLYDIYHMQIMQGNLCSFIEKNIDIIGHFHSAGVPGRHELFDGEI
                     HYPFIMKLLEKLGYKGCFGLEYMPAMNDHALSLKKTLEYLTT"
     gene            1440..2234
                     /locus_tag="PLW07_03335"
     CDS             1440..2234
                     /locus_tag="PLW07_03335"
                     /inference="COORDINATES: protein motif:HMM:NF013586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOL49589.1"
                     /translation="MELGYQFMQNILSYPVHENSEKLLLLPEIAKKSGVKIKFSSARR
                     VKVSKPIFRLREGLIENFLRAAEEMNRRGWIMKVEDAFRTRDMQNPLNRPRKIFDAIM
                     KIILWECEGKIPEPEFIFRRLTVLIATIPKIATHMSGSAIDISAVSMKNGNEIDRGAD
                     YLEMNEKTFMDSIFVSEKAKENRRIITEIMQEHGFYAYPFEFWHYSSGDAFAQSLSKS
                     GKVAVYGPVELIDDSGTVRPIENATEVLVKPEQLLEQLEKSLKRFH"
     gene            complement(2237..3427)
                     /locus_tag="PLW07_03340"
     CDS             complement(2237..3427)
                     /locus_tag="PLW07_03340"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOL49590.1"
                     /translation="MKFVAERPVTIHSVRYDTRWFPFVFKHTDGSLLLYIECGYDAHF
                     SPFFRLRSTDNGKTWEHPVANVPRVSTVHSFKDNTLFEMDTYAVQDAKEKDAYITYGA
                     WSNPGKPNGIVKKDFVKIYAPSFKPVSLEIFQKHGAYPTYPWWELFNLVTGKQEAGAS
                     DVFLGGVYFTSIIELEDETLIGLGYWYPKKAPELSKYALGCFESKDRGKTWMEKSIVA
                     YARNFSEGYCEATLVQLKDKRLYTVIRTGDYLYHTYSHDNGLTWKKPTQLTLVDSDIQ
                     PQKVWPVCKVLDDGTLVLVYGRPGKHIIFDPSGTGTQWQGHFDLQEWELDTQRLMGVP
                     EHSRLRGDTGNKCIRYWDSGDYLGLVVIGTNEMLVFYDVQNFVEHWNAYPVSGIRMVK
                     LRLE"
     gene            complement(3436..3984)
                     /locus_tag="PLW07_03345"
     CDS             complement(3436..3984)
                     /locus_tag="PLW07_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49591.1"
                     /translation="MKKNGTGGEKTTTGLNFEKKVDLQHLLSEIPGYQLKQIPGKAGT
                     YVFFEGEIVARCFRKNNFYKFLSENNINWQKLISKKLLPDDALLVIVRETLFIIEVKY
                     QQVPGSVDEKLQTCDFKRKQYLKLVTPLGLKVEYVYVLNDWFKKPQYKDVLEYINSVN
                     CHYKFNKLPLSWLGLPVKHVET"
     gene            complement(3981..4772)
                     /locus_tag="PLW07_03350"
     CDS             complement(3981..4772)
                     /locus_tag="PLW07_03350"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018029403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOL49592.1"
                     /translation="MCTGIWIYQDDCIHFMDTMLEKFQDGIFDMIFADPPYFLSNGGI
                     TCHAGKMTKVDKGEWDRSRGVEQNHDFNIAWLSRCQKLLKPNGTIWVSGTHHVIYSVG
                     FAMQQLGMKILNDITWEKPNPPPNLSCRYFTHSTETIIWSGKNNKCKHTFNYEIVKQM
                     SGGRQMKTVWRIPSPGKEEKLFGKHPTQKPLKLLERIILASTNEYDLIFDPFLGSGTT
                     AVAALKLKRKFVGCELEKSFVELAIKRIKQILQSAANEIPLDRCK"
     gene            complement(4894..6120)
                     /locus_tag="PLW07_03355"
     CDS             complement(4894..6120)
                     /locus_tag="PLW07_03355"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOL49593.1"
                     /translation="MVNQLVIIGAGPAGIMAAISAATEGKKVLLLDRNKSIGQKLLIA
                     GAGRCNVTSDISIEEFSRYYHNGDFLRNAFSQFSNVDLMNFFTENGMALDTERGHRVF
                     PATKSSADIVAFFRNMLEKHKVQLWFRNKVLKLEKQQDGIFKIISKKGEILAKSVILA
                     TGGKSYPQTGSKGDGYNLVRNLGHTIIKPVPVLCGINLKETWIKQWQGITLKNVIVKA
                     MSEGKIIGEEFGEAIFTHFGISGPAILNLSRNLADTRNKQGVKIIIDFKPALEEKILE
                     KRLIEEFQKNSNKSLKNIMKNLLPQNIIEEFLLMGTLSGDIKGNQVTAEQRKILISLL
                     KNFCLNVDSLRGFDEAIVTRGGVNVKEVNPKTMESKIVPGLFFAGEILDIDGKTGGFN
                     LQAAFSTGFVAGKHVG"
BASE COUNT         1906 a         1215 c         1113 g         2055 t
ORIGIN      
        1 tggtccaatt ataaaagcat taaaagagat ttcataccag ggatatattt ctgttgaagt
       61 gtttgacttt tcgccgggtc cagaaaatat cgcagaaaaa agtattcaat acctgaaaaa
      121 atttctctga ttatttaacc aatagaaaca ttgatttctg gcttaatcag tattgattgt
      181 ttgacaatac agttatttac tgcttccatc acgccttttt tatgttcttc tggtaaacca
      241 ggaatggaaa ttttcacctc aattgttgaa attctgtccg gatttttggt tttctcaaaa
      301 tcagcatcaa ttttcagacc ttctgcagga attctatttt ttgtacaata aaaaagcgca
      361 taatatccca tgcaggaagc aagacttaac acaaaaaatt ctggaggcat gggaccagta
      421 tctgttccct gcaattctgc aggtaaatct attgcaatac agtgctgtct gatttgtgcc
      481 tcgaatttta acctgtttgc cagttttact gttattgctg ccataattct cctttgtttg
      541 ctggaaatta ggttgctatc atgataaaat tgtacatcat aaaacaaaaa ttgaaaattt
      601 caaggagaga cacatgataa aaattgatgt ctgtatagat actgttttcc ctgaattaaa
      661 agatgaggaa aaaattgaga aagtaaaaga aattggttat caatgcgtgg aattctggtt
      721 tcacgacaat aaaaatgttg aagcgattgc aaaagcactg aaaaattctc aggtcaaact
      781 caacaatctt gttgtaaact cccctgatgg ctcacgaggt ggttctcttg tgaaaaagga
      841 agaccaacag acatatcttg aaaggctaaa atacgtaata tctattgcaa aatcacttga
      901 ttgtaacatg ggaattacat gtacaggtaa ttctataccg gatttatcaa aaagccagat
      961 gatggataat attattgaaa cattgagtat ggcgtgtgat attcttgaaa aagaatgttt
     1021 tactcttgtg cttgaaccat taaatacact tgtaaaccac aaaggatatt ttcttgactc
     1081 tgcaacagac gcagcagaaa ttataagaaa agttaataat cagcgtgtaa aacttcttta
     1141 cgatatctat cacatgcaga ttatgcaagg aaatctctgc agttttattg agaaaaacat
     1201 cgatattatt ggacattttc actctgcagg agttcctgga agacacgaac tttttgatgg
     1261 tgaaatccat tatcccttca tcatgaaact gcttgaaaaa cttggttaca agggatgttt
     1321 cggtcttgaa tatatgcctg caatgaacga ccacgcatta tctctgaaga aaacgcttga
     1381 gtatcttaca acctgatatg agacgcaaca tagagcggat tacttattgg aagcaacaga
     1441 tggaacttgg ttatcaattc atgcaaaata ttctttcata ccctgtgcat gaaaacagtg
     1501 aaaaactttt gttattgcct gagatagcaa aaaaatctgg tgtaaaaata aaattttcat
     1561 ctgcccggag agtaaaagta tcaaaaccta tcttcagatt acgagaaggt cttatagaga
     1621 attttttgcg cgctgcagaa gaaatgaata gacgcggctg gatcatgaaa gttgaggatg
     1681 cgttcagaac aagagatatg caaaatccct taaacagacc aagaaaaata tttgatgcga
     1741 ttatgaaaat tattttgtgg gaatgtgaag gaaaaatccc tgaaccagaa tttatcttca
     1801 ggcggttgac tgtgttaatt gcaaccatac caaagattgc cacacatatg tctggtagcg
     1861 ccattgatat ctctgcggtt tccatgaaaa atggtaatga aattgataga ggagcagatt
     1921 accttgaaat gaacgaaaaa acatttatgg actcaatctt tgtttcagaa aaggcaaagg
     1981 aaaacagacg aatcataact gaaattatgc aggagcatgg tttttatgca tatccttttg
     2041 aattctggca ttacagcagc ggtgacgctt tcgcacaatc tttgtcaaaa tccggtaagg
     2101 ttgctgtgta tggaccagtg gagctaattg atgattcagg tacagtaaga ccaattgaga
     2161 atgctactga agttcttgta aagcccgaac aattactgga acaactggaa aaatccctta
     2221 aaagatttca ttaaaattat tcaagccgta gtttcaccat tcttatccct gaaacagggt
     2281 atgcattcca gtgttcaaca aaattctgga catcataaaa aacaagcatt tcgtttgttc
     2341 caatgaccac caaaccaaga tagtcgccag aatcccagta tctgatacac ttgttgcctg
     2401 tatctcctct caacctcgaa tgctcaggca cgcccatcaa cctttgcgta tcaagttccc
     2461 attcttgaag gtcaaaatgt ccctgccact gggttcctgt tccagaaggg tcaaaaataa
     2521 tgtgtttgcc tggtcttcca taaacaagta caagggtccc atcatcaagc actttacata
     2581 ctggccagac cttttgcggc tgaatatctg agtcaacaag tgttaattga gttggctttt
     2641 tccaggtaag tccgttatca tgtgaataag tgtgataaag ataatctcct gtgcgtataa
     2701 cagtgtaaag ccgtttatcc ttcaattgca caagggttgc ttcgcaatat ccttctgaaa
     2761 aatttcgggc atatgcaaca atggattttt ccatccatgt ttttcctcta tcctttgatt
     2821 caaagcatcc taatgcatat tttgataatt caggggcttt ttttggatac cagtatccca
     2881 gtccaatcag ggtttcatct tcaagttcaa taatactggt aaaatatact ccgccaagaa
     2941 aaacatcaga tgcacctgct tcttgtttcc ctgtaacaag attaaaaagt tcccaccagg
     3001 gatatgtggg atacgcaccg tgtttttgaa aaatttccaa acttactggt ttgaaggatg
     3061 gggcataaat ttttacaaaa tcttttttta caatgccatt gggttttcct gggttactcc
     3121 aggcaccata ggtaatgtat gcatcttttt cttttgcatc ctgaaccgca taggtatcca
     3181 tttcaaataa cgtgttgtcc ttaaaactat gaactgtact aacccttgga acatttgcaa
     3241 ctggatgttc ccatgttttg ccattatcag tggacctcaa tctaaaaaaa ggagaaaaat
     3301 gcgcatcata gccacattca atataaagca gcaaactgcc atcagtatgc ttgaaaacaa
     3361 aaggaaacca tcgtgtgtca tacctgacgc tgtgaattgt tacaggcctt tctgcaacaa
     3421 atttcatata ctctcctaag tttctacatg tttaaccggc agaccgagcc acgatagagg
     3481 caatttatta aatttgtagt ggcaatttac actgttaata tattccagta cgtccttgta
     3541 ttgcggtttt ttaaaccaat cattcaaaac atacacatat tcgactttta gacctagtgg
     3601 agtgacaagc ttaagatatt gttttctttt aaaatcacag gtttgaagtt tttcgtccac
     3661 tgaacctggt acctgttgat atttcacctc aatgatgaaa agagtttctc ttacgatgac
     3721 aagcaatgca tcgtcaggca gcaatttttt tgagatcaat ttctgccaat ttatattatt
     3781 ttcacttaga aatttgtaaa aattattttt tctgaaacaa cgagcgacaa tttctccttc
     3841 aaaaaatacg taagtaccag ctttgcctgg tatctgtttt aattggtaac cagggatttc
     3901 tgagagcaaa tgttgtaggt ccaccttttt ttcaaaattc agtcctgtag tagttttttc
     3961 tccacctgta ccgttctttt tcatttgcac ctatccagag gaatctcgtt ggcagctgat
     4021 tgaagtattt gtttaatcct ttttatggca agttcaacaa aagatttttc aagttcacaa
     4081 cctacaaatt ttcttttcag ttttaatgct gcaactgccg ttgttccact acccaaaaac
     4141 ggatcaaaaa tcaagtcata ttcattggta cttgcaagaa taattctttc gagaagcttc
     4201 aatggttttt gtgttgggtg tttgccaaat aatttttctt cctttcccgg ggaaggtatc
     4261 ctccatacgg ttttcatttg tctccctccg ctcatctgtt ttacgatttc atagttgaag
     4321 gtatgcttgc acttattatt ttttccagac catataatag tttcagttga atgagtaaaa
     4381 tacctgcagg aaagatttgg aggaggattg ggtttttccc aggtgatatc attaagtatt
     4441 ttcataccta gttgctgcat ggcaaaacca actgaataga ttacgtgatg agtcccggaa
     4501 acccagattg taccgtttgg tttcaaaagt ttctggcatc ttgaaagcca tgcgatgtta
     4561 aaatcgtgat tttgctcaac tcctctggac ctgtcccact cacctttgtc aacctttgtc
     4621 attttaccgg catgacacgt aatacctcca tttgataaaa aatatggtgg gtccgcaaaa
     4681 atcatatcaa agataccgtc ttggaacttt tcaagcattg tatccataaa atgaatacaa
     4741 tcatcttgat atatccagat gccggtacac atatcagaga aaacaggtgg taaggaaatc
     4801 ccggaaaatt ttatgttatc aaccatattc gtttgttttg caaaacattg ttttatagtc
     4861 gtatcattat tataaaactg ggttttaata gtttcaacct acgtgttttc ctgcaacgaa
     4921 accggttgag aaagccgcct gcaggttgaa accacctgtt tttccatcaa tatcaagtat
     4981 ttcacctgca aaaaacagtc ctggtacgat ttttgattcc attgtttttg gattcacttc
     5041 tttgacattc actccgcccc gggtgacaat tgcttcatca aatcccctta aagagtcaac
     5101 gttcaaacaa aaatttttca aaagggaaat aagaattttc ctctgttctg ctgtgacctg
     5161 attgcctttg atatcacctg ataaagtgcc cattaacaga aattcttcaa taatattctg
     5221 aggcaaaagg tttttcatga tgtttttcaa acttttgttg ctgtttttct gaaattcttc
     5281 aatcaatctt ttttcaagta ttttttcttc aagtgctggc ttgaagtcaa tgatgatttt
     5341 taccccctgt ttgtttctgg tatctgccag atttctggat aaattaagta tcgcaggacc
     5401 agagattcca aagtgagtaa agattgcttc tccaaattct tccccaatga tttttccttc
     5461 agacattgcc tttacaataa catttttcag tgtgattccc tgccactgtt tgatccatgt
     5521 ttctttcaga tttattccgc acaacacagg tactggtttt atgatggtat gacccagatt
     5581 acgtaccagg ttgtatccat ctccttttga gccagtttgc ggataacttt ttccgcctgt
     5641 ggcaaggatc acactttttg caagtatttc tccttttttt gaaatgattt tgaagatacc
     5701 atcttgctgc ttttccagtt tcagcacctt attccgaaac cataactgga ctttatgttt
     5761 ttcaagcata ttcctgaaaa atgccacaat atctgctgat gattttgttg cagggaatac
     5821 tctgtgtccg cgctctgtgt ccagagccat accattttca gtaaaaaagt tcatcaaatc
     5881 aacattggaa aattgtgaaa atgcatttcg taaaaaatca ccattgtggt aataccggga
     5941 aaattcttct attgagatat cgctggtaac attacacctt ccagcacctg caataagaag
     6001 tttctgacca atgcttttat ttctatcaag aagtagaacc ttttttcctt ccgtggcagc
     6061 agaaatagca gccatgatac ctgcaggtcc tgctccaata ataacaagtt ggtttaccat
     6121 tatgatggtt tgatgttgta ctgctgttta atgtctgaaa tgcctcattc ctgaaaatac
     6181 cattgaaatg cccagtttat cacaggtttc tattacatct ttatctttca gagagccacc
     6241 tggttgaata atggcgctga ctcctgtttt tgctgcgact tcaatactg
//
LOCUS       DAORYU010000070         6170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_8790_length_6170_cov_5.017498, whole genome shotgun sequence.
ACCESSION   DAORYU010000070 DAORYU010000000
VERSION     DAORYU010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6170
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8790_length_6170_cov_5.017498"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="PLW07_03360"
     CDS             complement(<1..430)
                     /locus_tag="PLW07_03360"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOL49594.1"
                     /translation="MDVGKILEEKLLSFKPGSIIKGTVIRVDKEVIVDFGYKTEGYVL
                     REEFKSEQEKIKPGTQVDLIVESLDPDPNGLILLSKEKADVMLNWDKIEKNYEEDKPV
                     TGTILQRVKGGFKVDIGILAFLPGSQTDIKPVTNPSQFINV"
     gene            complement(446..1315)
                     /gene="ispH"
                     /locus_tag="PLW07_03365"
     CDS             complement(446..1315)
                     /gene="ispH"
                     /locus_tag="PLW07_03365"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOL49595.1"
                     /translation="MTRQIIVGKSIGFCFGVTKAVNTANRVLDQYGCLTTLGDLVHNP
                     IVMSELREKGLRVVEQVSETRNCPFIIRSHGLPPDVLQELKQYAARIYDATCPFVKKV
                     QGLVRKLSAQQYFIFLIGDPEHPEILAMKKIAGDNSLVMKPYQKFDTCSTSLKKWAVI
                     AQTTISLDLYRTAIKKIINRLPAEKLTIFNTICPVSVKRQSEALELAGKVDALIVVGG
                     RKSSNTRKLVYIGRKVNKNTFLIESSSEVRNLNLANFEKIGIISGASTDVRCIREMMD
                     ILRDHPAQKKYRR"
     gene            complement(1312..1938)
                     /locus_tag="PLW07_03370"
     CDS             complement(1312..1938)
                     /locus_tag="PLW07_03370"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOL49596.1"
                     /translation="MKNLSTEYPAGESNKILWMAAKILGFFIFKLFFHLDVYGKENIP
                     CSGKFVIAANHASFLDPPLLGYICPKPIAYFTKQEQLFGLFGMLITRLGAIPLSKSSM
                     GTSDIRMALNVIMKGKGLLIFPEGTRSRSGKFLPAKPGIGFIVVRTGVPVVPVCIKGS
                     FRAMPPGRNFIRPGKIIIRVGKPLSYNHQQTYEEVAQDVMRKIVELSQ"
     gene            complement(1895..2308)
                     /locus_tag="PLW07_03375"
     CDS             complement(1895..2308)
                     /locus_tag="PLW07_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49597.1"
                     /translation="MKNKKSHTTRKKLLCGKTLNNQRYYFLNQILEKNPDYIEALEIM
                     GDLCTKSGDFETVFAIDMRLAFLQPLEAIHYYNLACDCALLGKKDEALMYLNIAIILG
                     YRDFKHLNEDPDLASIREDERYEKLINGISCRRIK"
     gene            complement(2298..2552)
                     /locus_tag="PLW07_03380"
     CDS             complement(2298..2552)
                     /locus_tag="PLW07_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49598.1"
                     /translation="MFISKRIVLDTSTNWIYIGILEGEDHDYYYLSDADAFDVSEVNM
                     TKHEYLVRIKKDGLVINRRKTIVAKEKVIAITLLEDIIEK"
     gene            complement(2558..3004)
                     /gene="dtd"
                     /locus_tag="PLW07_03385"
     CDS             complement(2558..3004)
                     /gene="dtd"
                     /locus_tag="PLW07_03385"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010323597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOL49599.1"
                     /translation="MKLVIQRVIHARLTVDGVLKGAIDRGMVVFVGFGKNDHESLIEP
                     AVRKVLKLRIFADVHDKMNLSLLDISGGLMVVSQFTLYARTEGGNRPDFLEAMNPSEA
                     ETLFNRFVETCMKFYPGKIVGGDFGSQMLVEIHNDGPVTIVINWEK"
     gene            3108..3893
                     /gene="dapF"
                     /locus_tag="PLW07_03390"
     CDS             3108..3893
                     /gene="dapF"
                     /locus_tag="PLW07_03390"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOL49600.1"
                     /translation="MKITGSGNDFVMFNNLDGKIENRRKLAIEVCRFKYGIGADGAIF
                     LEKSKKADFKMRIFNSDGSEAEMCGNGLRCLIRFIHEQKISRKRKLSIETLAGIYNTS
                     FHNPGVSIRMFLVEKPALHKVVEIDGEKLVVHSLNTGVPHAVIPVENVKNVDVKKYGP
                     LVRYHKIFQPAGTNVDWLEVRDSHHGKVRTYERGVEDETLACGTGIVASVLCGAMLGK
                     FTSPVEIEARSGEKLKVAFSKDFNEIFFEGGTKLVFEGNWINR"
     gene            3935..6046
                     /locus_tag="PLW07_03395"
     CDS             3935..6046
                     /locus_tag="PLW07_03395"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOL49601.1"
                     /translation="MASGILIQKLFSIPFEYLLLLFIFSGIISLCLTLIFKKISWIFL
                     VFFTVITGIFLAGNAGTGYEKTLPIPPEKIEAITGIVKDVREKGPLLLITLEDTMVWD
                     NHQWIEANVTIHTALTGNSQIYNYDRIIFHQIKKIKQEDGRVTISSKSWYRVKNDECI
                     SGYPQFSQKITKVTENIIKKWFKYHGQEAAILKMMVLGNKQQSLEIKHIFVKTGTYHL
                     LIVSGLHLGYVLLFLRILFFLFRHIEQAHYKIFNFLYLIAIGMYAAITGFSTPVLRAA
                     LMLAVYLVADIIERPINGIESIGWAGFIILFVKPEELFNMGFQLSFAATTGIMLTIRN
                     TPRIKRIPEWLDTSIRAIAGAQIFTIPILAGHTGSVYPAGFFTNFFLVPAGGLVVCLG
                     LGFFVFGFLRSILIFPLIKALDLFWITTKLFSNFSPEIPWSPDIISVLIAYSIVLMLL
                     FRNKWKFFAGILLLLIAMSMMSMQKKEVPQQILIESRRHLDERITIFPAKKLFCCVEK
                     EHCMIFIVSEKEDETTLNPVLDNLPETNKNIVYFFTAPAHDIIGNLECLMSRAKPVVI
                     IDNPDIRKHPAFGYRKYFIVGDSHIKQQFWKFLTPYQGIKIVYDDGKNMVIEYHSKKG
                     TILIATYLNAQIFEVLPFSPRYHAVYATELALSKKLAEYLQEYEISRLIYQKTRYNTL
                     DKMDALPDFKIIEMKGIFTID"
     gene            complement(6033..6167)
                     /locus_tag="PLW07_03400"
     CDS             complement(6033..6167)
                     /locus_tag="PLW07_03400"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOL49602.1"
                     /translation="MSFGIACFPSDAQDSEGLIKMADDALYEAKKLGRNRVVVSVNQS
                     "
BASE COUNT         1884 a         1276 c         1098 g         1912 t
ORIGIN      
        1 ggacgttgat aaattgagat ggatttgtca ctggcttgat gtctgtttgc gaacccggaa
       61 gaaacgcaag aattcctata tccaccttga aaccgccctt gaccctctga agaattgtcc
      121 ctgtaaccgg cttatcttct tcataatttt tttcaatttt atcccagttc agcataacat
      181 ccgctttttc ctttgaaagc agtatgaggc catttgggtc aggatcaagg gattcaacaa
      241 tcaggtctac ctgtgttcct ggttttattt tttcctgctc gcttttgaac tcttcgcgaa
      301 gaacatatcc ttcggtcttg taaccaaaat caacgattac ctccttatca accctgatta
      361 cagtaccctt aatgatagaa ccaggcttaa acgatagcag tttctcttcc agaatttttc
      421 caacatccat accctccata ttgtgttacc tcctgtattt tttttgcgct ggatggtctc
      481 ttaaaatatc catcatctcc ctgatgcacc tgacatccgt agacgcacca gaaattatac
      541 caattttttc aaaatttgca agattcaagt ttcttacctc tgaagaagat tcaatcaaga
      601 atgtattttt attgactttt ctaccgatat aaacaagttt tcttgtgttt gaactctttc
      661 tacctcccac aacaatcaga gcatccactt tacctgccag ttcaagcgct tcagactgtc
      721 tttttacact cacagggcag attgtattga aaattgtgag tttctcagca ggtagtctgt
      781 taataatttt tttgatggca gttctgtaaa gatcaaggga tatggtcgtc tgcgcgataa
      841 ctgcccattt tttcaaactg gttgaacagg tgtcaaattt ttgatatggc ttcattacca
      901 gagaattgtc acctgcaatt tttttcatcg ccagtatttc aggatgttcg ggatccccga
      961 ttaaaaaaat aaaatactgc tgcgcagaca attttcttac cagaccctga actttcttta
     1021 cgaatgggca ggtggcatca taaattcttg ctgcgtattg tttcaattct tgaagaacgt
     1081 ctggcggaag tccatgggac cttattataa atggacagtt tctggtttca gatacttgct
     1141 ccacaactct caaacccttc tccctcagtt ctgacatgac gataggatta tgaacaagat
     1201 ctccaagggt tgtaagacat ccgtattgat ccagaactct gtttgcagtg ttcactgcct
     1261 ttgtgacacc aaaacaaaaa ccaattgatt tacccactat gatctgtctg gtcattgtga
     1321 aagctccaca attttcctca tcacatcctg agccacctct tcatatgtct gttggtggtt
     1381 ataagacaaa ggttttccca ccctgataat aatttttccc ggtctgatga aattccttcc
     1441 aggcggcatc gccctgaaag aaccttttat acaaacagga accacaggca ctccagtacg
     1501 aactacaata aaacctatac ctggcttggc aggtaaaaac ttacctgacc ttgaacgagt
     1561 cccttcagga aaaatcagca acccttttcc tttcattatc acattcaacg ccattcttat
     1621 gtcagatgtc cccatgctgg actttgaaag aggaattgct ccaagtctgg taatcaacat
     1681 accaaaaagg ccgaaaagtt gttcctgctt ggtaaaatac gcaatgggct tcgggcaaat
     1741 gtatcccaga aggggtgggt caagaaaact tgcatggttg gcagcaatga caaactttcc
     1801 tgaacaggga atattttcct tcccatagac atcaagatga aaaaaaagtt tgaaaataaa
     1861 aaacccgagg atttttgcag ccatccacaa aatcttattt gattctcctg caggatattc
     1921 cgttgataag tttttcatat ctttcgtcct ctctaatact ggcaaggtcc ggatcttcat
     1981 tcaaatgttt gaaatctcta taaccaagta tgatggcaat gttcaaatac atcaatgcct
     2041 cgtccttttt ccccagtaaa gcacaatcac aggcgagatt gtaatagtga atggcttcaa
     2101 gtggttgcaa aaaagcgaga cgcatatcaa tggcaaacac tgtttcaaaa tcccctgatt
     2161 tcgtacacaa atcgcccatt atttcaagcg cctcaatata atcaggattt ttttcaagaa
     2221 tctgattgag aaagtaatat ctttgattgt tcaaggtttt tccacatagc aatttttttc
     2281 tggtggtatg cgatttttta tttttcaatg atatcctcca gcagtgttat tgctattact
     2341 ttttcttttg ctacaattgt tttcctgcga ttgataacaa gcccatcctt ttttatcctc
     2401 acaagatatt catgttttgt catattgact tcagaaacat caaaagcatc ggcatctgaa
     2461 agataataat aatcatgatc ttcgccttca agtattccta tgtaaatcca gttagttgaa
     2521 gtatccagga caatcctctt tgaaataaac atatccttca tttttcccag ttaatgacaa
     2581 ttgtaacagg tccatcgttg tgtatttcaa caagcatctg gctgccaaaa tctcctccaa
     2641 caatttttcc aggataaaac ttcatacacg tttcaacaaa tctattgaac aaggtttctg
     2701 cttccgaagg attcattgct tcaagaaaat caggtctatt acctccttct gttctcgcat
     2761 aaagagtaaa ttgtgaaact accatcaaac cgcctgagat atcgagcaaa ctcaaattca
     2821 ttttatcatg tacatctgca aaaattctca atttcaaaac ctttcggact gctggctcta
     2881 tcaaagattc atgatcgttc tttccaaacc caacaaaaac aaccataccc ctgtcaatag
     2941 cacctttcag tacgccgtcc actgtcagac gagcatgaat aaccctctgg ataacaagtt
     3001 tcatatatta tcttccccat gataggaata actgaaatag tttattccag acagacctat
     3061 ttgacaaacg ctcgcaataa aattaaatta tttgcatgct taaatttatg aaaattactg
     3121 gttcgggtaa tgactttgtc atgttcaata acctggatgg gaaaatagaa aaccgcagga
     3181 agcttgccat tgaggtctgc agatttaaat atggtatagg cgcagatggt gcaatatttc
     3241 tagaaaaaag caaaaaggca gatttcaaaa tgcggatttt taattcagat ggttctgaag
     3301 cagaaatgtg tggcaatggg ttaaggtgtt tgattcgctt tatccatgaa caaaaaattt
     3361 caagaaaacg aaagttgtca atagaaacac ttgcaggtat ttacaacacc agttttcaca
     3421 atccaggtgt ttcaataaga atgtttctgg tggaaaagcc ggcattacat aaagtggtgg
     3481 aaattgatgg agaaaaactc gttgttcact cactcaatac cggggtacct cacgcagtaa
     3541 ttcctgttga aaacgtgaaa aacgtggatg taaaaaaata tggtccgctt gtaagatacc
     3601 ataaaatctt tcaaccagca ggcaccaatg ttgactggct tgaagtgaga gatagtcatc
     3661 atggaaaagt tcgcacatac gaacgtgggg tcgaagatga aacactcgcc tgcggaacgg
     3721 gaattgttgc aagcgttctc tgcggtgcaa tgcttggaaa attcaccagc cctgttgaga
     3781 ttgaagcccg ttcaggagaa aaactgaagg tcgcattttc aaaagatttc aacgaaatat
     3841 tttttgaggg tggaacaaaa ttggtttttg agggaaactg gattaacagg tgattttcgg
     3901 taatattccc agatcttgca ttttcatatt ccctttggct tcaggcatcc tgatacagaa
     3961 attgttttca attccattcg aatatctatt actcttattc attttttcag gcatcatttc
     4021 cttatgtctt acgctcatat tcaaaaaaat ttcctggatt ttccttgttt ttttcaccgt
     4081 tatcacagga atatttcttg caggcaatgc tggtactggt tatgaaaaaa ccctgccgat
     4141 tcctcctgag aaaattgaag ccataacagg tatcgtaaag gatgtaaggg aaaaaggacc
     4201 tctccttttg atcacccttg aagacacaat ggtatgggat aaccatcagt ggattgaggc
     4261 aaatgttaca attcacacag cgttaacggg aaatagccaa atctacaatt atgatcggat
     4321 tattttccat caaataaaaa aaatcaaaca ggaagatggc cgggttacaa tctcatcaaa
     4381 atcctggtat agggtgaaaa acgatgagtg catctcagga tatccgcagt tcagtcaaaa
     4441 aattactaaa gtgacagaga atataataaa aaaatggttc aaatatcacg gtcaggaagc
     4501 cgcaatcctc aaaatgatgg tgttgggaaa taaacagcag tcacttgaaa ttaaacacat
     4561 ctttgtgaaa actggcacat atcatcttct gattgtttca ggacttcatc tagggtatgt
     4621 cctgcttttc ctcagaatcc ttttttttct attcaggcac attgaacaag cacattataa
     4681 aattttcaat ttcctgtatc ttatcgcaat tggaatgtat gcagcaatca ctggatttag
     4741 cacccctgtt ctcagagcag ccctgatgct tgctgtgtat cttgttgcag atattatcga
     4801 aagacccatc aatggcatag aatcaatcgg gtgggctggt tttattatac tgtttgtgaa
     4861 acccgaagaa ctttttaaca tgggttttca actttctttt gcagcgacaa caggcatcat
     4921 gctcaccata cgtaatacac cgcggataaa aagaataccg gaatggcttg atacgtccat
     4981 cagagccatt gctggtgccc agatatttac aatcccgata cttgctggac atacaggaag
     5041 tgtttaccct gctggttttt tcacaaactt ttttctcgta cctgctggtg gacttgttgt
     5101 ctgtctgggg cttggttttt tcgttttcgg atttttaaga agcatattaa ttttcccact
     5161 gataaaagca ctggatttat tctggatcac taccaaactt ttttcaaatt tctcaccaga
     5221 aataccatgg tctccagata tcatttccgt gcttattgct tattccatcg tactgatgct
     5281 tcttttcaga aacaaatgga agtttttcgc agggatatta ctgttgctaa ttgcaatgtc
     5341 catgatgagc atgcaaaaga aagaagtgcc gcaacaaata ctgatagaat ctcgtcggca
     5401 tttagacgaa cgcattacaa tttttccagc aaaaaagctt ttttgctgcg tggaaaaaga
     5461 acattgtatg atttttattg tctcggaaaa agaagatgaa acgacattga atcctgttct
     5521 tgataatcta ccagaaacaa acaaaaacat cgtgtatttc ttcactgcgc cagcacatga
     5581 cattattggt aatttagaat gtcttatgtc acgcgcaaaa cctgttgtca ttattgacaa
     5641 tcctgatatt agaaaacacc ctgcgtttgg gtacagaaaa tattttattg tgggagacag
     5701 ccacataaaa caacaattct ggaaatttct tactccatac cagggaatta aaattgttta
     5761 tgatgacggg aaaaacatgg tgatagaata tcattcaaaa aaaggaacca ttctcattgc
     5821 aacatatctg aatgcacaaa tttttgaagt gctgccattt tcaccgcgat atcatgctgt
     5881 ttacgcaact gaactggcac tttcaaaaaa acttgcagaa tatcttcagg aatacgaaat
     5941 atcccggttg atataccaga aaacaaggta caatactctg gataaaatgg acgcattacc
     6001 ggattttaaa attatcgaaa tgaagggaat ttttacgatt gattaacact tacaacaacc
     6061 ctgtttctgc caagtttttt tgcttcatac aatgcgtcat cagccatttt gataagtcct
     6121 tcagaatcct gcgcgtcaga tggaaaacac gcaataccaa aacttattgt
//
LOCUS       DAORYU010000071         6131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_8857_length_6131_cov_5.225313, whole genome shotgun sequence.
ACCESSION   DAORYU010000071 DAORYU010000000
VERSION     DAORYU010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6131
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8857_length_6131_cov_5.225313"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..2327)
                     /locus_tag="PLW07_03405"
     CDS             complement(12..2327)
                     /locus_tag="PLW07_03405"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HOL49603.1"
                     /translation="MKKIRFAFGLVFVMCGIAFAKSAVICNATGWWDFPSFPLEEYKI
                     TEFRYYIPPERVNFPTNLTDKFIQDIKNSKLLYFGQSRNSIGPKKDMIFGNQSYRDAV
                     KNFLENGGTIIFDMGAPMEKDTLSFLTEIGVTIPKPGGIQGIPGEQIYPVISDVEKEK
                     NHPLVNFPYKLEPAEKLKWCGTYVSWAENQLAPLRASADPKYAVMIIQENVCGKGRVI
                     FNAVNNIFAHATRGEVYASNIFSYVYGEEIKRIPVANSRYKTDAQYTVWYKTPYSKFP
                     VEENAPEKNKTDRVEIKACINEVVGTNILITNSDREQLKFKVIAPELKDRIKGTVIPQ
                     EKLKIMELEFDTGRMPDKMPEKQEFIIGQGMTGIVWISLDTFELKDGEYEGDIVLQFE
                     KGQTKKIKMVVKIYPIELAKTNPLKLTVWDLVPGGPVREKMIGTPENWVNYHRDMAEH
                     GVNVFHLTSYEKPQLIFDADGRIVKEDFTRFDAGIPYRTKEYQYLVNMGSHTEEFRID
                     QQKETVKYGSENWEKFYREWIKSIIKHLKEIGLDYSQFAFYPYDEIGTKTVPDALKIY
                     SLIKQVDKKARIFVTVVPGGFFEAKEGLPVKEIAPYIDIWCPAVSYENYWSSGWASKE
                     QFDKNFEFMKGTGKEIWSYNVLTRGNQEIMAYKRYRLQPIGAYRLGITGYGFYGYNLW
                     KNDPYMVVYPGEKPITSYRWEAMREGMNDVKYIEDLKQEIKKSKNEKKKKEAEQLIEE
                     CLKNITQDNENAQLVYEYRDKIAEKILELRK"
     gene            complement(2314..4095)
                     /locus_tag="PLW07_03410"
     CDS             complement(2314..4095)
                     /locus_tag="PLW07_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49604.1"
                     /translation="MAERRDTLKENIFVNLPQEEKQKIFNQIENWQHSIRTTREIFAF
                     CLVLAEFGIYDGRIEKYLLVAEAQQDRNPGSATYGNFTWYIDSGKIVDLNSVEFCLRE
                     GLLLWLRHRNKLNEKTRDVLEGLLRYGINSVLNRKVRVRYTNIHLIKTWNMIAYGENF
                     KNKEIEKQGYEMLDEWLLYTRKNGIREYSSPNYYHNDITALGLIYNYTGNMEVKRKSE
                     MGLKLFWTDIALNFFKPAARIGGSRSRDYDYIYGRNGINRVLAHYGWIQKTDVSVFDY
                     LIALSEWYPPEEIKDLNSLQPRFIERKIGKDKGEWACHYAGKGFSIGCCGFCYSPLDK
                     PLVFLFTSDTLPNGYVVFDARNDPYAEKPEKQPWAGFEHEVCLHLVPFITANQNREEI
                     VMYSTIKADDPSIPRLAPDVKGFFTHFVFPACEIFLDGEKIELKNRESITLTINQVLI
                     VRYQSAAIGIRIFYATDLYGKNPEIYLIDDGNKYNVLRLTVVHSDRKMETGTGEIGFY
                     IVGKDDIDSSKIRSFCNEIRCIRFDVKKREDVMEISKDSTMILKFNPEKWERMIDRDV
                     QCILSVNGNDIGGKILEGMDRRKDEKN"
     gene            complement(4113..4952)
                     /locus_tag="PLW07_03415"
     CDS             complement(4113..4952)
                     /locus_tag="PLW07_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOL49605.1"
                     /translation="MEKKGFTLIELLVVIAIIAILAAMLLPVLSNAREKARRAVCINN
                     MKQMILALKMYSEDFNDLLPYPVTWCDQYQLANTDPGQGYVNMGLLLIGITGRAPLKL
                     NYLAGAEGFFCPSVGGPNGAYWKRTYFCRNAFYNKFETRTPAPSRTNICYNATEVVYR
                     SSANTITIPDWLGMGVKHIPTCSGKMTRVERFGYALVWDRWGSPDSWNHPGTRDTLPE
                     GLNVGFGDGSVVWVPDNNHRLFITFGGANTGRDTGYWYVYGSWVRERVEKDPLGPPPP
                     PRL"
     gene            complement(5146..>6131)
                     /locus_tag="PLW07_03420"
     CDS             complement(5146..>6131)
                     /locus_tag="PLW07_03420"
                     /inference="COORDINATES: protein motif:HMM:NF020121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HOL49606.1"
                     /translation="RNGGTVIADYQPGARDEHGKIYGKKCPLDEVFGIDRTKAEYVSR
                     KSKLSFLKDSLFGEKTIEMSMAETGLKTVSGKCMAVFEDGTPALVINQYGKGKGIYLN
                     LDISDYAGMKGRGVAGEVIEEEKGKIDYVLTVQEIFEKFLASAGLKKRVEILENGKPV
                     NAGERFYYTEGKNMYFAYIPEAVKETKVTVKTDGKLNVYDIRARKYIGNTDNFEDTIK
                     PGNVKVYSLLAYRVIDITGHLRSSYKQGETIELDAVLKTTSGKPDKHIFRIELYRDGK
                     NIQPYNKNIVAENGRAKIVIPLAYNQEKGNYSLLVRDINSGLDRVFNFRVI"
BASE COUNT         1593 a         1372 c          988 g         2178 t
ORIGIN      
        1 agcgaaaatt tttactttcg caattcgagg attttttcag cgattttatc cctgtactca
       61 tagacaagtt gagcgttttc gttgtcctga gtgatatttt ttaaacattc ttcaattaat
      121 tgctctgctt cctttttctt tttctcgttt tttgactttt ttatttcctg tttcaaatct
      181 tctatgtact tcacatcatt catcccttct ctcattgctt cccatctgta tgaagtgatt
      241 ggtttttctc ctggataaac taccatgtat ggatcgttct tccataaatt gtacccataa
      301 aatccatacc cggttatacc caatctatat gctccgattg gctgcaatct gtaccttttg
      361 tacgccatga tttcctggtt tcctcgtgtg agaacattgt aagaccatat ttctttgcct
      421 gtacccttca taaattcaaa atttttatca aactgttcct ttgaagccca tccgcttgac
      481 cagtaatttt cataagaaac agcaggacac catatatcaa tgtatggagc aatttccttt
      541 acaggcaatc cttcctttgc ttcaaaaaaa ccacctggca cgacagtcac aaatatccgt
      601 gcttttttgt caacctgctt gatgagtgaa taaattttca aagcatcagg cacagttttt
      661 gttcctattt catcgtaggg atagaacgca aactggctgt aatcaagccc aatttccttg
      721 agatgcttta tgatgctttt tatccattcc ctataaaatt tttcccaatt ttctgaacca
      781 tattttactg tttccttttg ctggtctatc ctgaattctt cagtgtggga acccatattg
      841 acaagatact gatactcttt tgtcctgtaa ggaatacctg cgtcaaatct tgtaaaatcc
      901 tctttgacaa tcctaccatc tgcatcaaaa atcaactgtg gtttttcata cgatgtcagg
      961 tgaaaaacat ttactccgtg ttcagccata tctctgtggt aatttaccca gttttctggc
     1021 gtgccgatca ttttttctct gacaggacct cctggtacaa ggtcccatac ggttagtttc
     1081 aaaggatttg tctttgccag ttcaattgga tagattttca caaccatttt tatttttttt
     1141 gtttggcctt tctcaaactg tagaacaata tctccttcat actcgccgtc ttttaattca
     1201 aaagtatcca gacttatcca gacaatgcct gtcatgccct ggccaatgat aaactcctgt
     1261 ttttctggca ttttgtctgg cattcttcct gtgtcgaact ccagttccat gattttcagt
     1321 ttttcctgcg gtattacagt accttttatc ctatctttta actctggtgc tatgaccttg
     1381 aacttcaatt gttctctatc agaattcgta atcaggatat tagttccgac aacctcgttg
     1441 atgcatgctt ttatttctac tctatctgtt ttattttttt caggagcatt ttcttcaacg
     1501 ggaaatttgc tgtacggtgt tttataccag acggtatatt gtgcatctgt cttatacctt
     1561 gaatttgcca cagggattct cttgatttct tctccataaa cgtatgaaaa aatgttcgag
     1621 gcgtaaactt cgcctcttgt tgcgtgtgca aagatattat taacagcatt aaatatcacc
     1681 cttccttttc cacatacatt ttcctgaatt atcattaccg catatttagg gtctgctgat
     1741 gcacgaagtg gcgctaactg attttctgcc caggatacat aggtgccgca ccatttcagt
     1801 ttttctgctg gttcaagttt ataaggaaaa ttgaccaggg gatgattttt ttccttttca
     1861 acatcagaaa tcactggata aatttgttct cctgggattc cctgaatgcc acctggtttt
     1921 ggtattgtta ctccaatttc tgtcagaaaa ctcaatgtat ccttttccat tggtgcgccc
     1981 atatcaaaaa ttattgttcc accattttca aggaaatttt tcacagcgtc cctgtaggat
     2041 tgattgccaa aaatcatatc ttttttagga ccgatgctgt ttcttgattg tccgaaataa
     2101 agaagtttcg agtttttaat gtcctgaata aatttgtctg tcaggttggt tgggaaattc
     2161 actctttctg gcggaatata atacctgaat tcagtgattt tgtactcctc aagtggaaac
     2221 gaaggaaaat cccaccatcc tgtcgcattg catatcactg ctgattttgc aaatgctatg
     2281 ccgcacatca caaataccag cccaaaagca aatctaattt ttttcatctt tcctcctgtc
     2341 catgccttcc agaattttgc ctccaatatc gtttccattc acgctgagaa tacattggac
     2401 atctcggtca atcattcttt cccatttttc aggattaaat ttcaaaatca ttgttgagtc
     2461 ctttgaaatt tccataacat cttccctttt tttgacatca aaacgtatgc accggatttc
     2521 gttgcagaaa gacctgattt tgcttgaatc gatatcatct tttcctacaa tgtaaaaacc
     2581 gatttcacct gtccctgttt ccatttttct gtcagaatga acaactgtca atcttaatac
     2641 attatattta ttaccatcat ctatcaggta aatttcaggg ttttttccgt acaggtcagt
     2701 agcataaaaa atcctgatgc ctattgcggc agattgatat cgtacaatta aaacctggtt
     2761 aattgtcaat gtgattgatt cgcggttttt taattcaatt ttttccccat ccagaaaaat
     2821 ttcgcaggca ggaaaaacaa aatgagtaaa aaaacctttc acatcaggag ccaatcttgg
     2881 tatagaaggg tcgtctgcct ttatagttga atacatcaca atttcttctc tgttttgatt
     2941 tgcggtaatg aacggtacaa ggtgaagaca tacttcgtgt tcaaatccag cccatggctg
     3001 tttttctggt ttttctgcgt aggggtcgtt tcttgcgtca aaaacaacat atccatttgg
     3061 caatgtgtcc gaagtaaaaa gaaaaacaag gggtttatcc agcggactat aacagaaacc
     3121 gcaacaacct atgctgaatc cttttcccgc ataatggcat gcccactcac ctttatcctt
     3181 tcctattttt ctttcaataa atcttggttg caaactgttt aaatctttta tttcttcagg
     3241 cggataccac tcagaaagtg caataagata atcaaaaaca gacacatctg ttttttgaat
     3301 ccatccataa tgggcaagaa ccctgttgat tccatttctt ccgtaaatgt aatcatagtc
     3361 cctgcttctt gaaccaccaa ttcttgccgc tggcttgaaa aagtttagtg caatgtctgt
     3421 ccagaataat ttcagaccca tttcactttt tcttttcact tccatgtttc ctgtataatt
     3481 gtaaatcaaa ccaagggcag tgatatcgtt gtgatagtag tttggagaag aatattccct
     3541 tatgccattt tttctggtgt atagcaacca ttcatcaagc atttcataac cctgtttttc
     3601 aatttcttta tttttgaaat tttctccgta tgcaatcata ttccatgttt ttatcagatg
     3661 gatattggtg tatctgacac ggactttacg attcaataca ctgtttatgc cgtatcttaa
     3721 caacccttca aggacatctc tggttttttc gttcaatttg tttctgtgtc tgagccagag
     3781 caaaagccct tctcttagac agaattcaac tgaattaagg tcaacaattt ttccagaatc
     3841 aatgtaccag gtgaaattgc catacgttgc gcttccggga tttctatcct gttgggcttc
     3901 agcaaccagt aaatactttt caattcttcc atcgtaaatg ccaaattcag ccagcacaag
     3961 acagaaggca aaaatttccc ttgttgtgcg tattgaatgc tgccagtttt caatttgatt
     4021 gaaaatcttt tgtttttctt cctgcggcag attgacaaaa atattctctt tcaatgtatc
     4081 tcttctttca gccatgttgt gctgaggatt atttatagcc ttggtggtgg cggtggacca
     4141 agggggtctt tttcaaccct ttctctcacc catgagccgt acacatacca gtaacctgta
     4201 tctctgccag tattcgctcc accaaaggtt ataaataagc ggtgattgtt gtcagggacc
     4261 cataccacag aaccatctcc aaaacccaca ttcaaaccct caggaagtgt atcccttgtg
     4321 cctggatggt tccatgaatc cggacttccc catctgtccc agaccagggc atatccaaat
     4381 cgttcaaccc ttgtcatttt tcctgaacag gtcggaatat gcttgacccc cataccaagc
     4441 cagtcgggta ttgtgattgt attggcgctt gaccgataca caacttctgt ggcattgtaa
     4501 catatatttg tccttgaagg agcaggtgtc ctggtttcaa atttgttgta aaacgcattg
     4561 cggcagaaat atgtcctctt ccagtatgca ccatttggac cgccaacaga tggacagaaa
     4621 aatccttctg ctccagcaag ataattcaac ttcaatggtg ctcttcctgt tattccaata
     4681 agcaacagtc ccatgttcac atagccctga cctggatctg tattggcaag ttgatactgg
     4741 tcgcaccatg tcacaggata tggaagcaaa tcattgaaat cttcagaata cattttcagc
     4801 gcaagaatca tctgcttcat gttattgata cacaccgctc ttcttgcttt ctctcttgca
     4861 tttgaaagta ctggcaaaag cattgctgca aggatggcaa tgatggcaat caccacaagc
     4921 aattcaataa gcgtaaaacc tttcttttcc atggctgctc cttttttcgt gcctgaaatc
     4981 aggcattaaa atatgtcatc ggtaacgcga gaggattgag tccatgaaac tggaatgttt
     5041 ttttattgca ggaaaatgaa aataatccca aagcgtatag aatttttttc tggcatgaca
     5101 cggtaaacct ttcatatttt ctctcttgtt accgtatttt cattgttata ttaccctgaa
     5161 attgaaaacc ctgtcaagcc ctgaattgat atcccttacc agcagggaat aattgccctt
     5221 ttcctgatta tatgccagcg gtatgacaat tttcgccctg ccattttcag ccactatgtt
     5281 tttgttgtat ggctgaatat ttttgccatc cctgtacagt tctattctga aaatatgttt
     5341 gtctggtttt ccagaggttg ttttcaacac tgcatccagt tcaatggttt ctccctgttt
     5401 atagcttgac ctcaaatgcc cggttatatc tataaccctg taggcaagca gtgaataaac
     5461 ctttacattt cctggtttaa tcgtgtcttc aaaattgtct gtatttccaa tgtattttct
     5521 tgctctgatg tcatacacat ttaattttcc atctgttttc acagtcactt ttgtttcttt
     5581 tactgcttca ggaatgtaag caaaatacat gtttttccct tctgtataat aaaatctttc
     5641 acctgcattg actggcttac cattttccag tatctcaact ctttttttca atccagcgct
     5701 ggcaagaaat ttttcaaaaa tttcctgtac agtcaaaaca tagtcaattt tccctttttc
     5761 ttcctctatc acctctcctg caactcctct tcctttcatt ccagcataat ctgatatatc
     5821 gaggttgaga taaatacctt ttccctttcc atattgattg atgaccagcg caggagtgcc
     5881 gtcttcaaat actgccatac actttcctga aactgttttc agtccggttt cagccatact
     5941 catttcaatg gttttttctc cgaacaaact atctttcaaa aatgataatt ttgatttcct
     6001 gctgacatat tctgcttttg ttctatctat tccaaaaact tcgtcaagag gacatttttt
     6061 accataaatc tttccgtgtt catcccttgc tcctggctga taatcagcaa tcacagttcc
     6121 tccatttctc a
//
LOCUS       DAORYU010000072         6091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_8926_length_6091_cov_5.395792, whole genome shotgun sequence.
ACCESSION   DAORYU010000072 DAORYU010000000
VERSION     DAORYU010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6091
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8926_length_6091_cov_5.395792"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            203..1834
                     /locus_tag="PLW07_03425"
     CDS             203..1834
                     /locus_tag="PLW07_03425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOL49607.1"
                     /translation="MYIKGIIFDFDGTIVSQEIDFKKIFLEIRQLASQYKLKEPEEML
                     PILEYLKKIQKLNKTKSREFLKGAHAILLARELEYSKNAKPIKGATEFLSQLKKSGIA
                     IGVVTRNSRSVVEKILKEKNIPFDVLLAREDVPEVKPHPSHIRLMIKKLGLKKTEVLV
                     AGDHMLDVLAAKKLGILSCGVLSGGKTHQDFIDVGADFVYNDITHLAVLLGLEKLPEG
                     KLEHQLLKYLIKKYCVLDTSVQTGPGIGMDAAIIKTSKDILLLKSDPITLVSKNIGEY
                     AVTINANDVACLGGIPRWLLTTIIFPAGTRFPVVEETFADISQECHKHNIAWVGGHTE
                     ISASVSQVVLCCAIAGEKLKHIKQIKKIKSGDALIMVKQAGIEGASILAREKPEIEKI
                     FPGAFKKAMHATKNPGLSIVKEAMIAWNTADVIRMHDPTEGGIACGIAEIAESLGCGF
                     ILNEKNIDFYKPARMFCDYLKMDVYGLISSGCLLVAVPARIAEKLINRYRKCGIRASV
                     IGYATKKKGVLMKTKTGQFKTIYCSVHDEILKKLP"
     gene            1888..2274
                     /locus_tag="PLW07_03430"
     CDS             1888..2274
                     /locus_tag="PLW07_03430"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOL49608.1"
                     /translation="MTDGHIKTILVAEDDPETRLILQQGLLRAGYRVVIAEDGEQALE
                     RFRESRPDLVLLDIDMPRLNGWEVLERLKSGWRSRRVPVVMVTGKTSDEDKIRGYELG
                     VDYYVTKPFNTQRLLPIIRNLVSGIK"
     gene            2294..3052
                     /locus_tag="PLW07_03435"
     CDS             2294..3052
                     /locus_tag="PLW07_03435"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOL49609.1"
                     /translation="MKICLCQFDIIWENKQGNKQKIQTLVSGLEKTQVDLLIFPETTL
                     TGFSFNEITVITREDIAFFADIAKTYNTHVCFGGIYDNKNMCFSINPEGTLISQTGKI
                     HLFSPSGEQAYHLPERTITSFTIGNTRLTPFICYDLRFAPLFWTPAEKTDIFIVIANW
                     PEKRTKHWKILLQARAIENQAFVVGVNRTGESPHEKYCGDSCVIDPWGNVVLDAENEE
                     GIFICYIQVEQLEEIRKSFPVLKDRLAFSEYEKL"
     gene            3039..3254
                     /locus_tag="PLW07_03440"
     CDS             3039..3254
                     /locus_tag="PLW07_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49610.1"
                     /translation="MKSYRRWHATVDNFPAERKTAPCRVSVPFTQEECRHLYFSVERP
                     DGSEVPAQSRVIRQHNRAGYKTFFEIC"
     gene            3414..4085
                     /locus_tag="PLW07_03445"
     CDS             3414..4085
                     /locus_tag="PLW07_03445"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOL49611.1"
                     /translation="MKLIDISGTIEQGMWNYGDALNNPIFSGPAITELATVEKNGFSA
                     HKIELSILTSTYLETPAHLLKGKKTIDRIPLEELFLPASVIKLKTKPPLSHITKKEII
                     GAGVNIKKGDCLVIYTGWYKRWNKKGFVTECPHFDSECMDWIVSKKIKVLASDIPCYD
                     DIQDPVDSKTLPQLRKLYRSGAMALAPVVNGDKIKQGRAEIIILPMNVKSFSASPARA
                     VVVYR"
     gene            4096..>6091
                     /locus_tag="PLW07_03450"
     CDS             4096..>6091
                     /locus_tag="PLW07_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOL49612.1"
                     /translation="MKDKLKWGIISTGRIAQTFADGVLHSDTGKLVAVASREKQKAQQ
                     FAEKFNIPSAYESYEELLADSNVEAVYIATPHPSHALWAIRTAEAKKHILCEKPIGIN
                     HAQAMAIVDAAIENDVFLMEAFMYRCHPQTKKLVELLRENIIGDVRVIQATFSFQATF
                     SPESRLFNNDSGGGGILDVGCYCTSMARLVAGVATGKEIAEPIELKGCGHLGATGVDE
                     WAVAIAKFPGDIVAQLSTGVAIAQENVVRIFGTKGYIFIPSPWVISRDGGESKIFVHI
                     YGQSSPVEVTVKTDKKLYSIEADTVAENIENRQARFPAMTWDDTLGNMKTLDMWRQSI
                     GLVYEMEKPERMTVPVWGRPLQPGKKYTMKYGEIKGLDKKVSKVLMGADNQLTFPHAA
                     VMFDDFVERGGNAFDTAFIYAGGLCEKLLGEWIKSRNIRKDIVLLDKGAHTPYCNPED
                     LTKQLLISLERLQTDYIDIYMLHRDNPDIPVDEFVDVLNQHKKAGRIKIFGGSNWTPE
                     RIDQANEYARKKGLEGFSVVSNNFSLARMLAPIWQGVVSSSDEKSRKWFEERQIPLIA
                     WSSQARGFFTGRYSPDDKSDAQMVRCWYSEDNFNRLERAKQLAEKLGVPTTAIALAYV
                     LNQKFPVFALIGPRSVIETRSSLKTLEIELTEDQLKWLNLE"
BASE COUNT         1982 a         1122 c         1349 g         1638 t
ORIGIN      
        1 catggattaa ccggtataac caggattacc cgcattcatc attaggatat atgacaccag
       61 tggaatttga acaaaaagag ataaaacaat cacctttaac caggtcgcct aattctccct
      121 tattttgctc ttgacaaatg gggggcagta cagaaatacg tccatatttc tttcacaggt
      181 gcaagcaggt taaaataata tcatgtacat taaaggaatt atctttgatt ttgacggaac
      241 aattgtaagc caggagattg acttcaagaa aatttttctt gaaatacggc aacttgccag
      301 ccagtataaa ctcaaagaac cagaagaaat gctgccaatt cttgaatacc tgaagaaaat
      361 acaaaaattg aacaaaacaa aatccagaga atttttgaaa ggcgctcatg ctattttgct
      421 tgctagggaa ctggagtatt caaaaaatgc aaaacccatc aaaggcgcga ctgaatttct
      481 ttctcaattg aaaaaatcag gtatcgcaat aggtgtcgta acaagaaaca gcagaagcgt
      541 ggttgagaaa atcttgaaag aaaaaaacat tccattcgat gtgttgcttg caagagaaga
      601 tgttcctgaa gtgaagccgc acccatcaca catcaggttg atgataaaaa aacttggttt
      661 gaagaaaact gaggttctgg ttgcaggaga ccacatgctt gatgtgcttg cagcaaaaaa
      721 acttggaatt ttgtcatgcg gggtgttgtc tggcggaaaa acacaccagg atttcatcga
      781 tgtcggcgct gactttgttt ataacgacat cacacatctt gctgtgctcc ttggcctgga
      841 gaaacttcct gaaggaaaac ttgaacatca attgctgaaa tacctgatca aaaaatactg
      901 tgtactggat acaagtgttc agacaggccc tggcattggt atggacgctg ccattataaa
      961 aacatcaaaa gacattttac ttctgaagtc agaccccatc acccttgtgt caaagaacat
     1021 tggcgaatat gctgtaacaa tcaatgccaa tgatgttgcc tgtcttggtg gcattccacg
     1081 atggctgctg acaaccatta ttttccctgc tggaacaaga tttcctgtag ttgaagaaac
     1141 attcgcggat atttcacagg aatgtcacaa acataacatt gcctgggttg gaggacatac
     1201 tgaaatcagt gcttctgtct cacaggttgt tctttgctgc gctattgcag gagaaaaact
     1261 aaaacatatc aagcaaatta aaaaaattaa atcgggcgat gcgctgataa tggtgaaaca
     1321 ggcaggaatt gaaggtgctt ctattcttgc aagggaaaag ccagagattg aaaaaatttt
     1381 tcctggtgct ttcaagaagg cgatgcatgc aacaaaaaat ccaggtctat ccattgtaaa
     1441 agaagcaatg attgcatgga acactgctga tgtcatcaga atgcacgacc cgacagaagg
     1501 gggtatcgca tgcggcattg cagaaattgc agaatctctt ggatgcggtt ttattcttaa
     1561 tgagaaaaac atcgattttt ataaacctgc cagaatgttc tgcgattatc tcaaaatgga
     1621 cgtgtatgga ttgatttctt ccggatgctt gctggtagcg gttcctgctc ggattgctga
     1681 aaaacttatt aatagataca gaaaatgcgg tatcagggca tctgttattg gatatgcaac
     1741 aaagaaaaaa ggagtactga tgaagacaaa aaccgggcaa ttcaagacca tttattgttc
     1801 tgtccatgac gaaattctca agaaattgcc ctgatgtatt ttctgcctga ttttgtccag
     1861 gggcctgttc tgttatataa tttaattatg actgatggac atattaaaac aattcttgtg
     1921 gctgaagacg acccagaaac acgcttgata ctccagcagg gtcttcttag ggctggttat
     1981 agggttgtga ttgcagaaga tggagaacag gcacttgaaa gattccgtga atccagacca
     2041 gaccttgttc ttcttgatat tgacatgcct cgacttaatg ggtgggaggt tctcgaacgg
     2101 cttaaatctg gatggagaag caggagggtc cctgttgtca tggtgaccgg aaaaacctca
     2161 gatgaggaca aaatcagagg gtatgaactc ggtgttgact attatgtaac aaaaccattc
     2221 aatacccaga gattgcttcc cattatcaga aaccttgtgt cagggattaa gtgatagtaa
     2281 ccgcttatct ccaatgaaaa tatgcctgtg tcagtttgat ataatatggg aaaataaaca
     2341 gggaaataaa caaaagatac aaacacttgt ttctggcctc gaaaaaacac aggttgacct
     2401 tttgatattt cctgaaacaa cgcttactgg tttttccttc aatgaaatca cagtcattac
     2461 gcgagaggat atagcatttt tcgcagatat tgcgaaaacg tataacactc atgtttgttt
     2521 tggtggtatt tatgataata agaacatgtg tttcagcatc aacccagaag gtactctcat
     2581 ttcacagaca ggtaaaattc atcttttttc accttcaggc gaacaggcat atcatcttcc
     2641 cgaaagaaca attacttcct tcacaattgg caacacacgc cttacgccat ttatttgtta
     2701 tgatttacgg tttgctcctt tattctggac acctgctgaa aaaacagata tatttattgt
     2761 tattgcaaac tggcctgaaa aaaggacaaa acactggaag atattgcttc aggcaagggc
     2821 gattgaaaat caggcatttg tggtaggtgt aaacagaaca ggtgaaagtc cgcatgaaaa
     2881 gtattgcggc gattcatgcg tgatagaccc atggggaaat gttgtgcttg atgctgaaaa
     2941 tgaagaaggc atatttatat gctatattca ggttgaacaa ctggaggaaa tacgtaaatc
     3001 tttccctgtt ttgaaagata gattggcttt ctctgaatat gaaaagttat agaagatggc
     3061 atgcaactgt tgacaatttt cctgcagaaa ggaaaactgc gccatgcagg gtgagtgtgc
     3121 catttactca ggaagagtgc agacatcttt atttttctgt tgaaagacct gatggttcag
     3181 aagttcctgc tcaatcaagg gtcatcaggc aacacaatag agccggatac aagacctttt
     3241 ttgaaatatg ttgaatatgg aatgatttag gatggatttc aggcatgggc aacctcagta
     3301 caaaaaaata cgcaagggta tacagaggac tcattcattt tgtctctggc tgtgctgatg
     3361 caggattgct tgaggatttt acgaaaactc tgtattctta aaaaggagga aacatgaaac
     3421 tgattgatat cagtggcacg attgaacagg gaatgtggaa ttatggagat gcgttaaata
     3481 atcccatctt ttcaggtcca gcaatcactg aacttgcaac tgttgaaaaa aacggattca
     3541 gcgctcacaa aatagaactt tccattctta catcaacata ccttgaaact cctgcacatc
     3601 tattgaaggg gaaaaaaacc attgaccgga ttccccttga agaacttttt cttcctgcaa
     3661 gtgtgatcaa actgaaaaca aagccacctc tcagccacat cacaaaaaaa gaaattattg
     3721 gcgcaggagt aaacataaaa aagggcgatt gtcttgttat ctacactggc tggtataaac
     3781 gatggaataa aaaggggttt gttacagaat gtcctcattt tgattctgaa tgcatggact
     3841 ggattgtttc aaaaaaaatt aaagtacttg cttcagatat accctgttat gatgatatcc
     3901 aggatcctgt tgattcaaaa acacttccac agttaagaaa actttatcgt tcaggagcaa
     3961 tggctcttgc gcctgtggta aatggagata aaataaaaca gggaagagca gagattatca
     4021 ttcttccaat gaatgtaaaa tctttcagtg caagtccagc aagagcagta gttgtttaca
     4081 gatgatggag gaaacatgaa agacaaatta aagtggggaa ttatcagtac aggaaggatt
     4141 gcacaaactt ttgcagacgg cgtgttgcat tcagatacag gaaaacttgt tgcggttgcc
     4201 agcagggaga aacaaaaggc gcagcagttt gcagaaaaat ttaatatccc ttcagcatat
     4261 gaaagttatg aggaattact tgctgatagc aacgttgaag cagtgtacat tgcaacaccc
     4321 catccatccc atgccctgtg ggcaattcgt acagcagagg caaaaaaaca cattctttgt
     4381 gaaaagccca tcggcatcaa tcatgcgcag gcaatggcta ttgttgatgc ggcaattgag
     4441 aatgacgttt tcctgatgga agcatttatg tacaggtgcc atccgcaaac aaaaaaactc
     4501 gttgaacttt tgcgagaaaa catcattggc gatgtcaggg ttatacaggc aaccttcagt
     4561 tttcaggcaa cttttagtcc tgaaagccgc cttttcaaca atgattctgg cggaggcgga
     4621 attcttgatg ttggttgtta ctgcacatcc atggcgcggc ttgtagcagg agttgctact
     4681 ggcaaagaaa ttgcagaacc gattgaatta aagggctgtg ggcatcttgg agccacaggc
     4741 gttgatgagt gggctgttgc gattgcaaaa tttcctggcg atattgtggc gcaactatca
     4801 acaggcgttg ccattgcaca ggaaaatgtt gtcagaatct tcggaacaaa aggatatatt
     4861 ttcattcctt ctccatgggt tatctccaga gacggcggag aatcaaaaat ttttgtacat
     4921 atttatggac agtcgtctcc agtggaagtg acagtgaaaa cagataaaaa actgtattcc
     4981 attgaagcag atacagtggc agaaaacatt gaaaatcgcc aggcaaggtt tcctgccatg
     5041 acatgggatg atacacttgg aaatatgaaa acactggaca tgtggcgtca gagcataggg
     5101 cttgtttatg agatggaaaa accagaaaga atgacagtgc cggtctgggg tcgtcccttg
     5161 caaccaggga aaaaatacac aatgaaatat ggagaaatca aaggacttga taaaaaggtt
     5221 tcaaaagtat tgatgggagc cgataatcaa ctgacatttc cccatgctgc agtcatgttt
     5281 gacgactttg tcgaaagggg tggcaatgca tttgatacag cgttcatcta tgcaggcggc
     5341 ttatgtgaaa aactgcttgg cgaatggata aaatcaagaa atatcagaaa ggacattgtt
     5401 cttcttgata aaggtgctca tactccttac tgtaatcctg aagacctgac caaacaattg
     5461 cttatcagcc ttgaaagatt gcagacagat tacatcgata tttacatgct tcacagggat
     5521 aaccctgata tacctgtgga tgaatttgtt gatgttctta accaacacaa gaaagcagga
     5581 agaattaaaa tttttggtgg ttcaaactgg acgcctgaaa gaattgatca ggcaaatgaa
     5641 tacgcaagaa agaagggact tgaaggattc tctgtcgtga gcaacaattt ttcccttgca
     5701 agaatgctcg cgccaatatg gcaaggtgtc gtttcaagtt ctgatgaaaa atcaagaaaa
     5761 tggtttgaag aaagacagat tcctctgatt gcatggtcaa gtcaggcaag aggatttttt
     5821 actggaagat attccccgga tgataaaagc gatgcgcaga tggtcagatg ctggtacagt
     5881 gaagataact tcaaccgtct cgaaagggca aaacaacttg cagaaaaact tggcgtccca
     5941 acaacagcca ttgcccttgc ctatgtgctc aatcaaaagt ttccagtatt tgccctcata
     6001 ggaccaagaa gcgttattga gacaaggagc tctttgaaaa cacttgagat agaattgaca
     6061 gaagaccagt tgaaatggtt gaaccttgaa a
//
LOCUS       DAORYU010000073         5935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_9219_length_5935_cov_5.015476, whole genome shotgun sequence.
ACCESSION   DAORYU010000073 DAORYU010000000
VERSION     DAORYU010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5935
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9219_length_5935_cov_5.015476"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1442
                     /gene="rny"
                     /locus_tag="PLW07_03455"
     CDS             <1..1442
                     /gene="rny"
                     /locus_tag="PLW07_03455"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOL49613.1"
                     /translation="EIIRDAENIALTRKKELDIQTKETLYRLRSEFEKETRNKRKELQ
                     FLERRLNQREILLEKKAELIEKREAEIATKEAGLAAKEHEIQQKISELENRIEQEIKK
                     LEEISGMTREQAKDTLLKAMEQEARNSSVEIIRKIEEEAREIGNKKAREILLASMQRL
                     ASEAATESTVSVVSLPNDEIKGRIIGREGRNIRAFEAATGVDLIVDDTPQAITISSFN
                     IYRREIARIALEKLISDGRIHPGRIEEVIQKTIQEMEEQLIEDGKNVAFDLGIDNLNP
                     EIVKLLGRLRYRTSFGQNVLQHAKESAYLAGMIAAEIGLNPKIARRAALLHDIGKALD
                     QEVEGSHPDIGADILKRAGESEEIVDAAMSHHKDLDIASPYTVIVQISDALSATRPGA
                     RRDTLESYLRRVEELEKIGSSFKGVEQAYAISAGREIRVIVKPEEISDQQAMLLARDI
                     ARKIEEDLKYIGQVKVTVIREKRETEFAR"
     gene            1494..2558
                     /locus_tag="PLW07_03460"
     CDS             1494..2558
                     /locus_tag="PLW07_03460"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOL49614.1"
                     /translation="MWNIIYVGVFLVSLCFSLMLVPVVRKFAIAFDVIDQPSLRKIHL
                     FPTPLSGGIAVYLAILATVIAGLMLKGIFPEKFFSHAAGVMHVLPKLTVFLVTSAVVV
                     IFGFLDDMYHLRPMVKLGFQIICGIITFLAGIKISFFAPYEWLQAIFTIGWIVVCMNS
                     FNLLDHANGLASGVACIAGGIFFFSAVSNGQLFISTLLACFIGAVSGFIPYNFPGAKI
                     FLGECGSSFLGYFLACLAMMGTYYRYGPDQSVLPALSPVLIFALPFFDTLSVMYLRIK
                     NRKPIFQADKNHIYHRLVKLGMNQVQAVIYCYLLTFATGLGALLLRRLNTIGGMIVFF
                     QVVLILVCIAMLESTGRRNG"
     gene            2551..3252
                     /gene="pyrF"
                     /locus_tag="PLW07_03465"
     CDS             2551..3252
                     /gene="pyrF"
                     /locus_tag="PLW07_03465"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOL49615.1"
                     /translation="MAELIVSLDIREKSVLENLVKKIGDAVAYYKIGAIPFTAFGPDV
                     IHWLKRSGKKVFFDLKFFDIPNTVELAVECACSMGIDLLTVHIHGGRKMLESTKRARD
                     NTNPETKIIGVTVLTSFTGEDLINVGIRETMEHQLLHLADTGYQCGLDGIVCSPQDLK
                     LLRKKFPDNFLMVCPGIRPASSNDDQKRIATATEAVKSGADYLVVGRPIIQSPDPLAV
                     VEQIRKEIKENEQNS"
     gene            3236..4315
                     /gene="alr"
                     /locus_tag="PLW07_03470"
     CDS             3236..4315
                     /gene="alr"
                     /locus_tag="PLW07_03470"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOL49616.1"
                     /translation="MSRILEIDLETVSNNVKVLKRFTGKKILACVKSDGYGMGAIPVS
                     KKIEKTVDWFGVATIDEGISLRKAGIIKPILVLGASLQPDVRKAIDYRVSLTAADASF
                     IRNIINAKGLSIHLKVDTGMGRIGIMPEEVSNVMNVIKNSKGIKLEGIFSHLATSESM
                     DRRYAIKQIEIFKKVIAGLPAKLRGITHIANSGGIVNLPESIKGFSMVRTGLLLYGVY
                     PSLFLKIFKSIPNLKYALKGTAQILSIRKVPAGTRISYGGTFVCKHDTTIAIAGIGYA
                     DGLNRALSNRFYMKHNEQLIPVRGNICMDQTVVELREEARKGDQLIFLDQQISVEDMA
                     EICSTVPHAIMTGFGVSRLRKVYHG"
     gene            4308..5102
                     /locus_tag="PLW07_03475"
     CDS             4308..5102
                     /locus_tag="PLW07_03475"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOL49617.1"
                     /translation="MDEKDINKMIKMVDAYLEKCLAFTSSRARGLERAMYYAVFPGGK
                     RIRPLLVLAVAKILNIPSKKILPVACGIELIHSFSLVHDDLPCMDDDYFRRGKPSCHK
                     KFGEAEALLAGDALLCLGIREIAKCGEPEAVIITCDALGSDGMAGGQSLDMIYKNKKI
                     PDRTKIWIDEKKTGELFRLCFEIPAIVGKSDRVILKKLRQTGALFGEAFQILDDIHDR
                     EGNEKHLAMRLNNTYSRLLHQSENFGSRADTLTALIKMIFGGFVLG"
     gene            5152..5922
                     /locus_tag="PLW07_03480"
     CDS             5152..5922
                     /locus_tag="PLW07_03480"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOL49618.1"
                     /translation="MAIIETKQLTKHYFLGKKKVIVGLDKVNLQVEQGEIFGILGPNG
                     SGKTTCLKLLLGILFPTSGEARIMDKFPDDIEMKQHVGFLPENPYYYDYLTGPELLQF
                     YASLFSMDADTTKKRVDELLELVELKDAQRLSLRHYSKGMLERIGLAASLINDPEILI
                     LDEPTTGLDPIGCRDTRNLLLKLKEQGKTILLSSHFLSEVERVCDRIAIFHRGYLLAT
                     GTLDGLNKQYQAANLEDLFVKTISTFDNQASVKTGETT"
BASE COUNT         1853 a         1046 c         1412 g         1624 t
ORIGIN      
        1 gggagattat cagggacgct gaaaacatcg cgttaaccag gaagaaagaa cttgatatcc
       61 agacaaagga aaccctgtac aggctccgct ctgaatttga gaaagaaacc agaaacaaga
      121 gaaaggaatt gcagtttctt gaaaggagat tgaatcagag agagatactg cttgagaaaa
      181 aggcagaact tattgaaaag agggaagccg aaatagcaac caaggaagca ggtcttgccg
      241 caaaagagca cgagatacag cagaaaatca gtgaactgga aaacagaatt gaacaggaaa
      301 tcaagaaact ggaagaaata tcaggtatga caagggaaca ggcaaaagat actcttctta
      361 aggcgatgga acaggaggca agaaacagct ccgtggagat tatccgcaag atagaagagg
      421 aggcaaggga aataggaaat aagaaagcaa gggaaatttt acttgcgtcc atgcaaagac
      481 ttgcttcaga agcagcaacc gaaagcactg tttctgttgt aagtttgccc aatgacgaaa
      541 tcaagggaag gatcattggt agggagggga gaaacattcg cgcctttgaa gcagcgacag
      601 gtgttgattt gattgtggac gatacgcccc aggcaattac gatttcttct tttaacattt
      661 acagaagaga aatagcaaga atcgcccttg agaaattgat ttcagacggt agaatacatc
      721 ctggaagaat tgaggaagtc attcaaaaga ccattcagga gatggaagaa caacttattg
      781 aggatggcaa gaatgttgcc tttgaccttg gaattgacaa tcttaatcca gagatagtga
      841 aattgcttgg cagattgaga tacagaacaa gttttggaca gaatgttctc cagcatgcaa
      901 aggagtcggc atatcttgca ggaatgatag ccgctgaaat tggactcaat cctaaaatag
      961 caagacgggc tgctcttctg catgatatcg gtaaagccct tgatcaggaa gttgaaggca
     1021 gtcatcccga cattggcgca gacattttga aaagagctgg agagtcagaa gaaattgttg
     1081 atgcggcgat gagtcatcat aaagaccttg atatagcgtc cccatacact gtcatcgttc
     1141 agatttcaga cgcattgtca gccaccagac ccggagcaag aagggatacc cttgaaagtt
     1201 atctccgtcg cgttgaggaa cttgaaaaaa ttggttcttc tttcaaaggg gttgagcaag
     1261 catacgcaat atctgctgga agggaaataa gagtcattgt gaaaccagag gagatttctg
     1321 atcagcaggc aatgcttctg gcaagggaca ttgcgagaaa aatagaagag gacctcaaat
     1381 atattggtca ggtcaaggtt actgtcataa gggaaaaaag ggaaacagaa ttcgcaagat
     1441 agtataatgc tggtttaaca ggatcggggc gtagacagcc gccccgctga aatatgtgga
     1501 acatcattta cgtgggtgtg tttctggttt ctttgtgttt ttccctgatg cttgttcctg
     1561 tggtaagaaa gtttgccatt gcatttgatg ttattgatca gccgtctttg agaaaaatcc
     1621 acctgtttcc aaccccactt tcaggcggga tagccgtata tcttgctatc cttgccactg
     1681 ttatagcggg gctgatgtta aaggggattt ttccagaaaa atttttcagc catgccgcag
     1741 gggttatgca tgttttacca aaactgactg tgtttcttgt tacatcagca gtggttgtga
     1801 tttttgggtt tcttgatgac atgtaccacc tgaggccaat ggtgaaatta gggtttcaga
     1861 tcatctgcgg aattataaca tttcttgcag gaataaaaat ttccttcttt gcgccctatg
     1921 agtggttgca ggcaattttc actattggct ggattgttgt ctgtatgaac agtttcaatc
     1981 ttcttgacca cgccaatggc cttgcttcag gggttgcgtg cattgccggg ggtatatttt
     2041 tcttttccgc ggtctccaat ggacagttat ttatttccac attgcttgca tgttttattg
     2101 gagccgtgtc agggtttata ccatataatt ttccaggggc aaaaattttc cttggtgaat
     2161 gcggaagtag tttccttgga tattttcttg catgccttgc aatgatgggg acatactatc
     2221 gttatggacc agaccagagt gtgcttcctg cattgagccc tgtactcatt tttgctcttc
     2281 ctttttttga taccctgagt gtgatgtatc tgaggataaa aaatagaaaa cctattttcc
     2341 aggcagacaa aaaccacatt tatcacaggc ttgtgaaact tggcatgaat caggttcagg
     2401 cagtcattta ctgctattta ctcacgtttg ccacagggct tggagcgctt ttgctgagac
     2461 gtctcaacac gataggcggc atgattgttt tctttcaggt ggttttgatt cttgtctgca
     2521 ttgcaatgct tgaaagcaca ggaagaagaa atggctgaat tgattgtaag tctcgatatc
     2581 agggaaaaaa gtgtccttga gaaccttgtg aaaaaaattg gggatgctgt tgcttattac
     2641 aaaatcggag cgattccttt tactgctttt ggccctgatg ttattcactg gttgaaacgt
     2701 tcaggaaaaa aggtattttt tgacctgaag ttttttgata ttccaaatac ggttgaactt
     2761 gccgttgagt gtgcctgcag tatgggtatt gaccttttaa ccgttcatat acatggcgga
     2821 agaaaaatgc ttgaatctac aaaaagagcc agggataaca ctaatccaga aacaaaaatc
     2881 ataggagtga cagttctcac ctcatttacc ggtgaagacc ttatcaatgt gggtatcagg
     2941 gaaaccatgg aacatcaact cttacatctt gctgatacag gatatcaatg cgggcttgat
     3001 ggtattgtat gttctccaca ggacctgaaa ttactgagaa aaaaatttcc tgataatttt
     3061 ctcatggtat gtccgggcat aagaccagca agttcaaatg atgaccagaa aagaattgcg
     3121 acagcaactg aagcagtaaa atcaggcgca gattatcttg ttgttggaag gccgattatt
     3181 caaagccctg acccactggc tgttgtggag cagataagaa aggaaataaa ggaaaatgag
     3241 cagaattctt gaaatagacc ttgaaacagt ttcgaacaat gtaaaggtgc tgaaaaggtt
     3301 taccggaaaa aagattcttg cctgtgtcaa aagcgatggt tatggaatgg gagccatccc
     3361 tgtaagcaag aaaattgaaa aaactgttga ctggtttggt gttgcaacga ttgatgaagg
     3421 aatatcgttg agaaaagcag gcattataaa accgatactt gttcttggtg cttcacttca
     3481 gccagatgtg agaaaagcaa ttgattaccg tgtatctctt actgcggcag atgccagttt
     3541 catcaggaat attattaatg caaaaggtct ttcaattcac ctgaaggtgg ataccggcat
     3601 gggaagaatt ggcattatgc ctgaggaagt cagtaatgta atgaacgtaa taaaaaattc
     3661 aaagggaata aaattagaag gaattttttc tcatctggca acttcagaaa gcatggacag
     3721 gagatatgcc atcaaacaga ttgagatttt taaaaaagta attgccggat tgccagcaaa
     3781 attgcgtggt atcacacaca ttgcaaactc tggcggaatt gtcaatcttc ctgaaagtat
     3841 caaaggtttt tcaatggtca ggacaggact tttactctat ggcgtgtatc cttcactgtt
     3901 tttaaaaatt ttcaaaagca ttcccaactt aaaatatgcc ttaaagggta ctgcacaaat
     3961 tctgtccata agaaaagtgc ctgctggtac aagaatcagt tatggcggca catttgtttg
     4021 caaacatgat actaccattg cgatagcagg cattggctat gctgatggtt tgaacagggc
     4081 gctttccaac agattttaca tgaaacataa cgaacaactt attcctgtca ggggtaacat
     4141 ctgcatggac cagacagtcg ttgaattgag agaagaagcc agaaaaggag atcaattgat
     4201 ttttcttgat cagcagattt ctgtggaaga tatggctgag atttgcagta cagtgccaca
     4261 tgcaatcatg actggatttg gtgtatcgag attgcgaaag gtatatcatg gatgagaaag
     4321 acatcaataa gatgataaaa atggtagatg cctaccttga gaaatgtctg gcatttactt
     4381 ccagccgggc aaggggactt gaacgagcca tgtattacgc tgtgtttcct ggtggcaaaa
     4441 gaatcagacc gcttcttgtc ctggctgtgg caaaaattct gaatatacct tcaaagaaaa
     4501 ttttgcctgt ggcatgcggg attgaattga ttcacagttt ttctcttgta cacgatgacc
     4561 ttccctgtat ggatgacgac tatttcagaa gaggaaaacc gagttgtcat aaaaagtttg
     4621 gcgaggcaga ggcacttctt gcaggggatg cgctgctgtg tctcggtata agggagattg
     4681 caaaatgtgg agagccagag gcagtgatca taacatgtga tgccctcgga tcagatggaa
     4741 tggcaggggg tcaatccctt gacatgattt ataaaaataa aaagattccg gacagaacga
     4801 aaatatggat agacgaaaaa aagacagggg aacttttcag gttgtgtttt gaaattccgg
     4861 caattgtagg aaaatcagat agggttatac taaaaaaact cagacagacc ggagcattgt
     4921 ttggagaagc gtttcagatt cttgacgaca ttcacgacag agagggaaat gaaaaacacc
     4981 tggcaatgcg gttgaacaat acttattccc gacttttaca tcaatcagaa aattttggaa
     5041 gcagggcgga tactctgacg gctcttatca aaatgatttt tggcggtttt gtattgggtt
     5101 gaaattgtgc tacaataaat ttactcagat aacagaaggc agggagggat tatggcaatt
     5161 atagagacaa agcaactcac caaacactat tttcttggga agaagaaagt tattgtgggg
     5221 cttgataagg ttaatcttca ggttgaacag ggtgaaattt ttggaattct tggtccaaac
     5281 ggttctggaa aaacaacatg tttgaaactg ttgctcggga ttctttttcc aacatctggt
     5341 gaagcccgca tcatggataa atttccggat gatattgaaa tgaaacagca tgttggattt
     5401 ctgcctgaaa atccttatta ttatgattat cttactggtc cagaattgct tcagttctat
     5461 gcaagtttgt tttctatgga tgcagatacc acgaaaaaaa gggttgatga attgctggaa
     5521 cttgttgaat tgaaagacgc acaacgactt tccctgaggc attattcaaa agggatgctg
     5581 gaaagaatag ggcttgcagc cagtttaatc aatgaccctg aaattctgat tcttgatgaa
     5641 cccacaacag gtcttgatcc tataggatgc agggatacga gaaacctctt attgaaattg
     5701 aaggaacagg gcaaaaccat acttctttca agtcatttcc tttctgaggt tgaaagggtc
     5761 tgcgacagaa tcgcaatatt ccacagagga tacctgcttg cgacaggaac ccttgatggt
     5821 ttgaacaaac agtatcaggc agcaaatctt gaagacctct ttgttaaaac aataagtacc
     5881 tttgacaacc aggcctctgt taaaacagga gaaacaacat gaaaaaaatg tggct
//
LOCUS       DAORYU010000074         5838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_9405_length_5838_cov_4.744077, whole genome shotgun sequence.
ACCESSION   DAORYU010000074 DAORYU010000000
VERSION     DAORYU010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5838
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9405_length_5838_cov_4.744077"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(364..507)
                     /locus_tag="PLW07_03485"
     CDS             complement(364..507)
                     /locus_tag="PLW07_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49619.1"
                     /translation="MKNVIAGTILYFVCLVLPAMIDLPCKKMGGYQVDENLTVPYWLL
                     DET"
     gene            complement(521..2239)
                     /locus_tag="PLW07_03490"
     CDS             complement(521..2239)
                     /locus_tag="PLW07_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019535370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOL49620.1"
                     /translation="MNNIPRSEHPQPAFYRANWQNLNGEWDFEIDTSNTGIDKKFFLP
                     EKKFREKIIVPFPPESKLSGIQNTDFMVSVWYRRKLKIPETWRKKRIFLHFGAVDFLA
                     NVWLNSYHIGSHIGGYTPFSFDITDFLNNENMLVVNAYDDNRTYLQPCGKQSQLYHSY
                     GCYYTRVTGIWQTVYLEARPSNYIKHFRIYPDYDTGQVMFLVDIEGTGQLNIEIFAEN
                     EKICETKTNGTGTLFSFCTIKNFIPWSTENPFIYKVKFTLNAGSDHQDVVFSYFGFRK
                     IHIKGKKIFLNGKPIFMRMVLDQGYYPDGIYTAPSEQSLINDIKIAKQLGFNGARLHQ
                     KIFEPLFLYHADRLGYLVWDEYPNWGIKQRASHEASSVLINEWTDVINRDFNHPSVIG
                     WCPLNETHKPQDNHLIRQIYYMTKKFDPTRVVIDTSGYIHVETDIYDCHNYDQDPIKF
                     AADFEPFKAKDIVWQNNPDVDAPYKGQPYWVSEYGGIWWDEKGVKQGWGYGTRPASKK
                     EFLSRYKKLTEVLLNHPKICGFCYTQLYDVEQEVNGLYTYERRPKFDPDIIRKINSKK
                     AEIEKE"
     gene            complement(2254..3897)
                     /locus_tag="PLW07_03495"
     CDS             complement(2254..3897)
                     /locus_tag="PLW07_03495"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HOL49621.1"
                     /translation="MRNRADWVTGKPAIENRIVHIDLKGPKIPFDSFKNFIQLLARWG
                     INGVLVEYEHRFPYLPLKHQFPPNDRYTTFQLKELINIAQDRGIEWIPLVQTFGHLEY
                     LSRLKGTEDMFENPAYIQQLCPLNEKAREYLQKLIEIVCEFHPDSQYIHVGQDETFQL
                     GFCSRCKKEVEKNGKMSLYLKHVQWVWELVRRYGKKPMFWNDMFFTENCPELLSNLPG
                     NGIPVTWQYEDTSEISHDVIIGGIKVSKIDAKHPYKMPVYGNVVARFAKPGDFFEDLP
                     EHTRNIIGVDETTGYPNSFSQLKITAHYNKNFWATCAVYNCSDMLFSPSFIRGLFNPV
                     AMVNEVMKLGGKGIIASNWARAHSYAPISPPWTLTFYNTAHFAYAAYTGNTSSQDIKE
                     TGKLIAEELKMAWKYGEISLDDFLWIISADAPGPGNIRRIHQLTEVLNLLKTKHSRGV
                     FGQGLLISVEAQLLYTKLLFLQEEARWWNSTKKEIPVVIKQEIKKRFFQIRREIASLE
                     KKARNYYIQCVGDRKSFRTWWKGLFELDMYLTEKAISFL"
     gene            complement(3922..4509)
                     /gene="gmk"
                     /locus_tag="PLW07_03500"
     CDS             complement(3922..4509)
                     /gene="gmk"
                     /locus_tag="PLW07_03500"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF012832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOL49622.1"
                     /translation="MTSRQREKQLHKANLFVISAPSGTGKTTLVNKLKNQFEHLAVSI
                     SYTTRNPRKGEKHGIDYFFIAEEEFKQKISGGFFAEWAQVYDHYYGTSKHFIDENLAE
                     NKIVLLTIDTQGALQIKKKYPQAILIGILPPSMEEQKKRLEKRGETMENIKRRLAESS
                     KERKILMEQYQYRFINRNLENTAKKIAAVIKRFAG"
     gene            complement(4475..5164)
                     /locus_tag="PLW07_03505"
     CDS             complement(4475..5164)
                     /locus_tag="PLW07_03505"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOL49623.1"
                     /translation="MKRKKGYKYFLAIVVCFICIHLVQVQIDYRKKLENLESALVLMP
                     GQMAGSLILGGFKGLAADLLWLQMETYWHSGQHYKILPLLQAISWLQPSYILVWTIGG
                     WHMSYNIYAEVKTPEEKQFWYEQGIQFLKKGLAYPFNANKYDIYFELGWTYYHKGKDY
                     ANAVKYFEKAIKYPHPDYVDDVLAHAYEKNGQIDKAIKQWEYVRDNYLAFYDIAVRMI
                     NDLKTKGKATP"
     gene            complement(5161..>5838)
                     /locus_tag="PLW07_03510"
     CDS             complement(5161..>5838)
                     /locus_tag="PLW07_03510"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOL49624.1"
                     /translation="IVIGASKLFSFLTAEEEMKMIKDVSFSAIEFFGALIAIFGALYA
                     ISGEIERRTIYTLVTKPIRRSDVVIGKFFGVSMIIFVNFILATVFFLGLLILKKSPPD
                     IEVFKTLLLIFFELILIGSITLALGTFTSDAFNIICSFFLYIVGHLTSYGKELIERTE
                     NIIVKGLADMLYTLVPNYENFNIRDKIVVGLNVSWGYVGYTILYGILYTCIALFIALY
                     FFEKREI"
BASE COUNT         1647 a         1214 c         1062 g         1915 t
ORIGIN      
        1 gatttcaaga aatctccccc agcccatttt cgtccccgcc cattttcccc ctttgtaaaa
       61 acccttctct tattttcccc ctttacaaaa tctctctcct gatatccccc tttgcaaaag
      121 ggggattgag ggggatttca agaaatctcc cccagcccat tttcgtcccc gcccattttc
      181 ccccttttca aaagggggat tgagggggat tttttaaatc catcccagcc ttccttttac
      241 aaaggaagga gacctgaagt tggcgggatt ttttgaaatc tcccccagcc cctcttttat
      301 aaagaggggg gtagaaaagg ggggggttgc aaagggagga gacctgaagt tagaaggaaa
      361 aaattatgtt tcatccagta accagtaagg aactgttaaa ttttcatcaa cctgatatcc
      421 ccccattttt ttacatggaa gatctatcat agcaggcaat acaaggcaaa caaaataaag
      481 aatagtacct gcaataacat ttttcattat tatatatgct tcattctttt tctatttctg
      541 ccttctttga gttgattttc ctgataatat cagggtcaaa ctttggtctt ctttcgtatg
      601 tataaagacc attgacttcc tgctcaacat catatagttg cgtgtagcag aagccacaaa
      661 ttttgggatg attcaaaagc acttcagtca attttttgta ccgtgataaa aattcttttt
      721 ttgatgctgg tcttgttcca tagccccagc cctgttttac tcccttttca tcccaccata
      781 taccgccata ctcactgacc cagtatggct gtcctttgta cggagcatca acatcaggat
      841 tattctgcca gacaatatct tttgccttaa atggttcaaa gtcagctgca aactttatcg
      901 gatcctgatc ataattgtgg caatcataaa tatctgtttc aacatgaata taaccgctgg
      961 tatcaatcac aacacgggtc gggtcaaatt tttttgtcat gtaataaatc tgtcttatta
     1021 aatgattatc ctggggctta tgtgtttcgt tcaatggaca ccagccaatg actgatggat
     1081 ggttaaagtc cctgttgatg acatcggtcc attcgttgat gagaacagac gatgcttcat
     1141 ggcttgcgcg ttgttttatt ccccagttgg gatattcatc ccagacaaga taaccaagcc
     1201 ggtctgcatg gtaaaggaat aatggctcaa agatcttctg gtgcaatctt gctccattga
     1261 aaccaagttg ttttgcaatc ttgatgtcgt taatcaggga ttgttctgat ggtgctgtat
     1321 aaattccatc aggataataa ccctggtcaa gcaccattct cataaagatt ggtttgccgt
     1381 taagaaaaat tttctttcct ttgatgtgaa ttttcctgaa accaaagtat gaaaacacta
     1441 catcctggtg gtcgcttcca gcattcaggg taaattttac tttataaata aatggatttt
     1501 ctgtactcca gggaataaaa tttttgattg tgcaaaaaga aaaaagagtg cctgtgccat
     1561 tggtttttgt ttcacagatt ttttcgtttt cagcaaatat ttcaatgttc agttgtcctg
     1621 tgccttcaat atcaaccaga aacattacct gtcctgtgtc ataatcagga tagatacgaa
     1681 aatgcttaat gtaatttgaa ggccttgctt caagatacac tgtctgccag atacctgtca
     1741 cccttgtgta ataacatcca taggaatgat ataactggga ttgttttcca cacggttgaa
     1801 gatatgttct gttatcgtca taggcattga caaccagcat gttttcattg ttcaaaaaat
     1861 ctgtaatgtc aaaactaaat ggagtataac cacctatatg agaaccaatg tgataactgt
     1921 taagccagac atttgcaaga aagtccactg ctccgaaatg aagaaaaatt cttttttttc
     1981 gccatgtctc tgggatctta agttttctcc tgtaccagac agataccata aaatccgtat
     2041 tctgtatgcc tgaaagttta ctttctggcg gaaatggcac aatgattttc tccctgaact
     2101 ttttttcagg aaggaaaaat tttttatcaa tgccagtgtt tgacgtgtca atctcaaaat
     2161 cccattcgcc attgagattt tgccagtttg ctcgataaaa agcaggttgt ggatgttcac
     2221 ttcttggaat attgttcatt ttccatatcc ctttcaaaga aaacttattg ctttttctgt
     2281 caggtacata tcaagctcaa ataacccctt ccaccatgtt ctgaaagatt ttctgtctcc
     2341 tacacattga atgtaataat tgcgtgcttt tttctcaagt gaagcaatct cgcggcgaat
     2401 ttgaaaaaac cgttttttaa tttcctgttt aataacaaca ggtatttcct ttttcgtgga
     2461 attccaccac cttgcttctt cctgaagaaa aagcagtttt gtatacagta gttgtgcttc
     2521 aacactgatc agtaaaccct gtccaaaaac acctctggaa tgttttgtct tcaaaagatt
     2581 caacacttca gtcagttgat gaattcttct tatattgcct ggtcctggtg catcagcaga
     2641 aattatccag aggaagtcat caagggaaat ttctccatac ttccatgcca tctttagttc
     2701 ttcggctatc agttttccag tttccttgat gtcttgtgaa gatgtgtttc ctgtataggc
     2761 agcatatgca aaatgcgcag tattataaaa agtcagtgtc cacggcgggc tgattggagc
     2821 ataagagtgg gcacgagccc agttacttgc gataatgcct ttgccgccaa gtttcattac
     2881 ctcgttgacc attgccacag gattaaacag tcccctgatg aaacttgggg aaaaaagcat
     2941 gtcagaacaa ttgtacactg cacaggtagc ccagaagttt ttgttgtaat gggctgtgat
     3001 ttttagttga gaaaaactgt ttggataacc agtggtctcg tctacaccga taatattcct
     3061 ggtgtgttct ggaaggtctt caaaaaaatc tccaggtttt gcaaatcttg caacaacatt
     3121 tccatacaca ggcattttat agggatgttt tgcatcaatt tttgaaacct tgataccacc
     3181 aataatcaca tcatgggata tttctgatgt atcttcatac tgccaggtca cgggtatgcc
     3241 attacccgga agatttgata ataattcagg acaattttct gtgaaaaaca tatcgttcca
     3301 gaacatcggc ttttttccat accttcttac aagttcccag acccattgca catgtttgag
     3361 atacaaggac attttcccgt ttttttcaac ttctttctta catcgagagc aaaaacccaa
     3421 ttgaaatgtt tcatcctgac ctacatggat gtattgactg tcaggatgaa actcacagac
     3481 aatttcaata agtttttgaa gatactctcg tgccttttca ttcaaaggac acagttgctg
     3541 gatatatgcc ggattttcaa acatatcttc tgtacctttc aaccttgaaa gatattcaag
     3601 atgaccaaag gtctgaacaa gtggaatcca ttcaatacca cggtcctgtg caatgtttat
     3661 cagttctttc agttgaaatg ttgtgtatct gtcattgggt ggaaactgat gtttcaaagg
     3721 aaggtatgga aagcgatgtt catactcaac aaggactcca ttgatacccc atcttgcaag
     3781 cagttgaata aagtttttga aggaatcaaa gggaattttt ggtcctttca ggtcaatatg
     3841 aacaattctg ttttcaattg ctggctttcc agttacccaa tctgccctgt ttctcatttt
     3901 attttataca cctgccatcg cttaccctgc gaatcttttt atcactgctg caattttttt
     3961 tgctgtgttt tctaaattgc gattgataaa gcgatactgg tattgttcca taagaatttt
     4021 tctttccttt gatgattcag ccaatcttcg tttgatattt tccattgttt caccgcgctt
     4081 ttcaagccgt tttttttgtt cttccatgga gggaggaaga attccaatga ggattgcctg
     4141 tggatacttt tttttgattt gcagtgctcc ctgggtatca atggtcagaa gcactatttt
     4201 gttttcagca aggttttcat caataaaatg ctttgaagtg ccgtagtaat ggtcataaac
     4261 ttgcgcccat tcagcaaaga agccccctga aattttctgc ttgaattctt cttcagcgat
     4321 gaagaagtaa tcaataccat gcttttcacc ttttctcgga tttcttgtcg tataggatat
     4381 ggaaacagca agatgctcaa actggttttt taacttatta acaagcgtcg tttttccggt
     4441 tcctgatggt gcagagatca caaacaggtt tgccttatgg agttgctttt ccctttgtct
     4501 tgaggtcatt gatcatgcgg accgcgatat cgtaaaaagc caggtaattg tctctaacat
     4561 attcccactg tttgattgct ttgtctatct ggccattctt ctcataggcg tgcgctaaca
     4621 catcgtcaac atagtcagga tgaggatatt ttattgcctt ttcaaagtat ttcactgcat
     4681 ttgcataatc cttgcctttg tggtaatagg tccatcccag ttcaaaatag atatcgtatt
     4741 tgttggcgtt gaatggatag gcaagtccct ttttcagaaa ttgtattccc tgttcatacc
     4801 agaattgttt ttcttcagga gttttgactt cagcataaat attgtaagac atatgccatc
     4861 cgcctattgt ccagacaaga atgtaacttg gctgcagcca cgatatcgcc tgaagcaggg
     4921 gaaggatttt ataatgttgt ccgctgtgcc agtaagtttc catctgtaac cataataaat
     4981 ctgctgccaa ccccttaaat ccaccaagaa ttaaactacc tgccatctga cctggcatca
     5041 gcaccagcgc gctttcaaga ttttccagtt tcttcctgta atcaatctga acctggacaa
     5101 gatggatgca gataaaacaa acgacaattg cgaggaaata tttgtatcct ttttttcttt
     5161 tcatatttcc cttttttcaa aaaagtaaag agcaataaaa agcgcaatac aggtgtaaag
     5221 aatcccgtac agaattgtgt atccaacata tccccagctc acattgagcc ccacgacgat
     5281 tttatccctg atattgaagt tttcataatt tggtacaaga gtatacagca tatctgccag
     5341 ccctttgacg ataatgtttt ctgtcctctc tatcaattcc ttgccatacg aagtaagatg
     5401 cccgacgata taaaggaaga atgaacaaat aatattgaag gcgtcagaag taaatgttcc
     5461 gagagccagt gtgatactgc cgattaaaat cagttcaaaa aatatcaaaa gcagtgtttt
     5521 gaatacttct atgtctggcg ggcttttctt taatatcaaa agcccaagga agaacactgt
     5581 tgcaaggatg aaattaacaa atataatcat tgaaacgcca aaaaactttc caatgacaac
     5641 atcagacctt cttattggtt ttgtcacaag cgtataaatg gtacgacgtt caatctctcc
     5701 tgaaatggcg tagagagcac caaagattgc tatcagggct ccaaaaaatt caatcgctga
     5761 aaaactcaca tccttgatca ttttcatttc ttcttctgca gtgagaaacg aaaacagttt
     5821 ggatgctcct atcacaat
//
LOCUS       DAORYU010000075         5823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_9433_length_5823_cov_4.457698, whole genome shotgun sequence.
ACCESSION   DAORYU010000075 DAORYU010000000
VERSION     DAORYU010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5823
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9433_length_5823_cov_4.457698"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1762
                     /gene="rsxC"
                     /locus_tag="PLW07_03515"
     CDS             <1..1762
                     /gene="rsxC"
                     /locus_tag="PLW07_03515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOL49625.1"
                     /translation="NLPENSPIGIISCGLGIQTVAKLGENKRIRVIALADSVPQSDNA
                     TSIIGYHGIALGSEKCAGCAQCYLGMTGGLCPIVDCAKSLLNGPCGGAKDSKCEVNAE
                     KDCVWIEIFKRMEKQKRTISYPVKVRDYNKFGSSEEKKISTISFARRTESFYGGVHPS
                     EKKELTENLPVQEFPSSKYLFVFLSQHAGYPATALVKQGNIVKYGQKIAESSGLISAS
                     VHSPVSGKVVSIEEKTHPALLRKMPAVVIENDFSDEKDEMISGCADWQSAPKEKLTEL
                     VKEKGIVGLGGAMFPTHVKFFPPKKPIDVLIVNGCECEPYLNADNRLMIEHPEEILHG
                     IMIARKVLGVSSVVVAIEENKPDAIESMKKAIGEKQGFVLKEMKTKYPQGAEKMLIKS
                     TLGRRVPDGGLPLDVGVVVLNVGTIFSIYRAIVQGLPLVKRMITVSGEFEKQGNLEIR
                     IGTPFTDIFEFCKSKISENKDEYCLKMGGPMMGIMQTDFDSAVIKGTTGMILTKKPLV
                     DCSQQNTCIKCGRCVDVCPMELYPLYYAYYGSRQMWEKCVEYGVKNCIECGCCQYVCS
                     SKIALVDLIKQAKKNANNKT"
     gene            1743..2357
                     /locus_tag="PLW07_03520"
     CDS             1743..2357
                     /locus_tag="PLW07_03520"
                     /inference="COORDINATES: protein motif:HMM:NF023646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HOL49626.1"
                     /translation="MLITKLKPLNEIENYFEKKVFVFKCFGCKEVYFPEQEINNFIEE
                     RKTGILSIGEVDYLCNVDFSKVYIEKNADVIQNSDAVVVFSCGVGVQVVSKLLENEIV
                     VPGCDTCYLNGFQGLTVLECDCNQCGECYLNYTGGICPITSCAKNLLNGPCGGAKNGK
                     CEVNPEVDCGWIAIYERLRTIDKEQILVENKMFVRNFEKIIKGK"
     gene            2360..3241
                     /locus_tag="PLW07_03525"
     CDS             2360..3241
                     /locus_tag="PLW07_03525"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008868914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HOL49627.1"
                     /translation="MEKQTFSEKLKAGKFVITSEIGPPKGTNIEPHLKEAEHIRDKID
                     AFNVTDLQSSVMRLGSLATCRLLIERGMEPVFQITCRDRNRLALQSDLLSAYVLGVRN
                     VLCLTGDHTTLGDHPDAKPVFDLDAVSLLKVATDLMNGKDMKGNDLDGKPEFFPGAVV
                     SPGYEPVELQIIKMEKKIEAGAKFFQTQAVYDLNSFEKFMKMVEKYNVPILGGIVLLK
                     SAGMAKYMNEKVAGVSVPDRYINMMAQAKKEERVKVSVQIAAELIKGMKNLCQGIHIM
                     PLGWEKYVPLVLEASAL"
     gene            3248..4075
                     /locus_tag="PLW07_03530"
     CDS             3248..4075
                     /locus_tag="PLW07_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HOL49628.1"
                     /translation="MGYLSPPEIAVAIVESGKKKVALRFLPMAILGFLAGVYIGFGAQ
                     LYTMVTHDLTKYLGCGFSKFLGGSVFTVGLMLVVIAGAELFTGNCLILTGVLTRDVKI
                     KEMFGNWFVVYFANFIGSVFLAGVIYLSGQWKFNDMGVGTAIVSTAIAKINLTFPEAF
                     FRGIACNWLVCLAVVLAMAGKDTVSKIFGIYFPIMAFVASGFEHSVANMYFIPVALFV
                     KGNIALANTNFVGAINTLTWGNFIVKNLVPVTLGNIVGGAIFVGAAYYFAYLKKYSS"
     gene            4124..4196
                     /locus_tag="PLW07_03535"
     tRNA            4124..4196
                     /locus_tag="PLW07_03535"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4158..4160,aa:Glu,seq:ctc)
     gene            4212..4592
                     /locus_tag="PLW07_03540"
     CDS             4212..4592
                     /locus_tag="PLW07_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49629.1"
                     /translation="MTDSEIFRFLGIFYLAVSFGIIIDPGYYRKFLRSYCCELTAIFL
                     SGMIAMFAGYLIITRHNIWQNTPAVIITILGWLAFLKGIFIIVVPQAFIRIVEIFLKT
                     EKTVYLQAIGGLIIGMIFLLISCF"
     gene            4605..5648
                     /locus_tag="PLW07_03545"
     CDS             4605..5648
                     /locus_tag="PLW07_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49630.1"
                     /translation="MNFREYTGCLHIHLNPVVRDEYLDDMAADAGRAGLNFLILTPHT
                     PKNKKHKDYFLGEGYRNNVMVLAGEEADEKSVFNHLIVYGDKNWVGRQPIHEIIKQVE
                     NYSLLAFAAHPYGKHRIFGIESNHQWTKKDFLNSITGVEAWSLLFDFAGKTNPSNMIL
                     RYFQFPENLTGPCYFTLKLWDKILNEKRFVGVAGLDIHPLKYAFRYLDIKERFSYGFV
                     FKTLRNHILVDEEFTGDISKDRNIVLEALKKGRLFFANDLLADSRGFFFGSEDQTRTM
                     GDCISTGERLIIEVPEEVDIRIKNRVETICFEKTKKAVIKAAIEGFYRVEVYYRERPW
                     IFSNPVYVKGVRP"
BASE COUNT         1830 a          846 c         1360 g         1787 t
ORIGIN      
        1 aaaccttcct gaaaatagtc caattggtat tatttcttgt ggtctgggta ttcagacagt
       61 ggcaaaattg ggtgaaaaca aacgcataag agtcattgct ctggctgata gtgtgccgca
      121 gagtgataat gcgacaagca ttattggtta tcatggtatt gcgcttggaa gtgaaaagtg
      181 cgctggatgt gcccagtgtt atcttggaat gactggtggt ttatgtccaa ttgttgattg
      241 cgcaaaaagt ttgctcaatg gtccgtgtgg tggcgcaaaa gatagtaaat gcgaggtgaa
      301 tgcggaaaaa gattgtgtgt ggatagaaat attcaaaaga atggagaaac agaaaagaac
      361 aatttcttat cctgtcaaag tcagggacta taataaattt ggttccagtg aagagaaaaa
      421 aatctccact atttcatttg cgagaagaac tgaaagtttt tatggaggag tgcatccttc
      481 agaaaaaaaa gaactaacag aaaacttacc agtacaggaa tttccttcat caaaatatct
      541 gttcgtcttt ctttctcaac atgcgggtta tcctgctaca gcactggtaa agcaaggtaa
      601 tatagtgaaa tatggacaaa aaattgctga gagttctgga ttgatttcag catctgtgca
      661 ttcgcctgtt tctggcaagg ttgtttcaat agaagaaaaa acccatccgg cgctcttaag
      721 aaaaatgcca gcagttgtta ttgaaaacga tttttctgac gagaaagatg agatgattag
      781 tggttgtgct gactggcaga gtgcgcctaa agaaaaactc actgaactgg taaaagagaa
      841 ggggattgtt gggcttggcg gagcaatgtt tccaacccat gtaaagtttt tcccacccaa
      901 aaaaccaatt gatgtactta ttgtcaatgg atgtgaatgt gaaccatatt tgaatgccga
      961 caacagattg atgattgaac atcctgagga aatcttgcat ggaataatga ttgcaagaaa
     1021 agtgctgggt gttagtagtg tggtggtggc aattgaagaa aataagccag atgccattga
     1081 aagtatgaaa aaggcaatag gagaaaaaca gggatttgtt ttgaaagaga tgaaaacaaa
     1141 gtatccgcag ggtgctgaaa aaatgctcat caaaagcaca cttggtagac gggtgccaga
     1201 cggtgggctt cctcttgatg ttggtgttgt tgttctcaac gtgggaacta tattttcaat
     1261 ttatcgagct attgttcaag gattaccact ggtgaaaaga atgattactg tatctggaga
     1321 atttgaaaaa cagggaaacc ttgagataag aattggcact ccttttacag atatttttga
     1381 gttttgcaaa agcaagatat cagaaaataa ggatgaatat tgtctgaaaa tgggcggacc
     1441 catgatggga attatgcaga cagattttga tagtgctgtg attaaaggaa ccacaggaat
     1501 gattcttaca aagaagcccc tggttgattg ttcacagcag aatacctgta tcaagtgtgg
     1561 aagatgcgtg gatgtttgtc cgatggaatt atatcctctg tattatgctt attatggaag
     1621 caggcagatg tgggaaaaat gtgttgagta tggagtgaaa aattgtattg aatgtggttg
     1681 ttgccagtat gtctgttctt cgaaaattgc tcttgttgat ttgatcaaac aggcgaaaaa
     1741 aaatgctaat aacaaaactt aaacctctga atgaaattga gaattatttt gagaaaaaag
     1801 tgtttgtttt taagtgtttt ggatgcaaag aggtctattt ccctgaacag gagattaaca
     1861 attttattga agaaaggaaa acaggtatct taagcattgg tgaagtagat tatctttgca
     1921 atgtggattt ttcaaaggtt tatattgaaa aaaatgcaga tgttattcag aattcagatg
     1981 ctgttgttgt attttcttgc ggtgtagggg tacaggtggt atcaaaattg cttgagaatg
     2041 agattgttgt gccaggttgt gatacatgtt atttgaatgg ttttcaggga ctgacagttc
     2101 ttgaatgtga ttgtaatcaa tgtggtgaat gttatttgaa ttatactggc ggaatatgcc
     2161 ctattacatc atgcgcaaaa aatcttctca atggtccttg cggtggagca aagaacggaa
     2221 aatgcgaggt aaatcctgaa gttgattgtg gctggattgc catttacgaa aggttgagaa
     2281 caatcgataa agaacagata ctggttgaaa ataagatgtt tgtgagaaat tttgaaaaaa
     2341 ttattaaagg aaaataacca tggaaaaaca gaccttttca gaaaaattaa aagcaggcaa
     2401 atttgtcatt accagtgaaa taggacctcc gaaaggaaca aatattgaac ctcatcttaa
     2461 ggaagctgaa cacatcagag acaaaattga tgcgtttaat gtgacagatt tacagagttc
     2521 agtgatgcgg ttgggttctc ttgcgacatg cagattattg attgaaaggg gaatggagcc
     2581 agtgtttcaa attacctgca gggatagaaa caggctggct cttcaatcag atttattgag
     2641 tgcctatgtt cttggagtca ggaatgtatt gtgcttgact ggagaccata caacacttgg
     2701 cgaccatcct gatgcaaaac cagtgtttga tctggacgca gtttctttat tgaaggtcgc
     2761 tacggattta atgaatggaa aagatatgaa aggcaatgat cttgatggga aaccagagtt
     2821 ttttcctgga gcagttgtga gccctggtta tgagccagtt gaattgcaga tcataaaaat
     2881 ggagaaaaaa attgaagcag gtgctaaatt ttttcagaca caggcagtgt atgacctgaa
     2941 cagttttgaa aaatttatga aaatggttga aaaatataat gttcccatcc ttggtggcat
     3001 tgtactgctg aaatcagcag gaatggcaaa gtacatgaat gagaaagttg ctggtgtttc
     3061 agttccggat agatacatca atatgatggc tcaggcaaaa aaagaagaaa gagtgaaggt
     3121 aagtgttcaa attgcagcag aattaataaa gggaatgaaa aatttgtgtc agggcataca
     3181 cattatgccc cttggttggg aaaaatatgt tcctcttgta ctggaagcat cagcactata
     3241 aataattatg ggatatcttt caccaccaga aattgcagtt gcaattgttg aaagtggcaa
     3301 gaaaaaagtc gcactgcgat ttctgccaat ggctattctt ggatttctgg caggagtata
     3361 catcggattt ggcgcacaat tatatacgat ggttacacac gaccttacaa aatatctggg
     3421 ttgcgggttt tcaaaatttc ttggtggaag cgtttttacg gttggcctga tgcttgttgt
     3481 gattgcaggt gcagaacttt tcactggcaa ttgtttgatt cttactggtg tattaacccg
     3541 cgatgtaaaa ataaaagaaa tgtttggtaa ctggtttgtt gtatattttg caaattttat
     3601 tggttcagta ttccttgctg gagtgatata tctttccgga caatggaaat ttaatgatat
     3661 gggtgttgga acagccatcg tttcaactgc aatagcaaag ataaacctca cttttcctga
     3721 agcatttttc aggggaattg cctgcaactg gctggtgtgt cttgcagtgg ttttagccat
     3781 ggcaggtaaa gatactgtga gcaaaatttt tggtatttat tttcctatca tggcgtttgt
     3841 tgcaagtggt tttgaacaca gtgttgccaa tatgtatttt atccctgtgg ctctttttgt
     3901 taagggaaat atcgctctgg caaatacaaa ttttgttggt gcaatcaata ctctcacgtg
     3961 gggcaatttt attgtgaaga atcttgtccc tgtgacactg ggaaacattg ttggcggagc
     4021 gatttttgtt ggtgccgcct attattttgc gtatctgaaa aaatactctt cctgaaaaat
     4081 ttgtcgtttt ccattctgta ttataaaatt atggtctgta aaagccccca tcgtctagcg
     4141 gcccaggaca ggtggttctc agccatcaga cacgggttca aatcccgttg ggggtactaa
     4201 taggaaatgt tatgaccgac agcgagattt ttagatttct cgggattttt tatctggctg
     4261 tatcctttgg aataattatt gatcccgggt actatagaaa atttcttcgt tcgtactgtt
     4321 gtgaactgac agcaattttt ttaagcggaa tgattgcaat gtttgctgga tatttaatca
     4381 ttacccggca taatatatgg cagaacactc cggctgtgat tataacaatt ctggggtggc
     4441 ttgcgttttt aaaaggaata tttatcattg ttgttccaca ggcgtttata agaattgttg
     4501 aaattttttt gaaaaccgag aaaacggttt atctgcaggc aataggtgga ttgattatcg
     4561 ggatgatttt tcttttgatt tcctgttttt gaatatagat ttcaatgaat ttcagagagt
     4621 acactggttg tttgcatatt catctgaatc cagttgtgcg ggatgaatat cttgatgata
     4681 tggctgctga tgctggcagg gctggattaa attttctcat tctcacaccc catacaccaa
     4741 aaaataaaaa acataaagat tattttcttg gtgaaggata tagaaataac gttatggtgc
     4801 ttgcaggaga ggaggcagat gaaaaaagcg tttttaacca tcttattgtg tatggcgata
     4861 aaaactgggt gggacgacag ccaattcatg aaataatcaa gcaggtggaa aattattctt
     4921 tgcttgcatt tgctgcccat ccttatggaa aacaccgtat ttttggaatt gaaagcaatc
     4981 atcaatggac aaaaaaagat tttctcaaca gtattacagg agtggaagcg tggtctcttt
     5041 tgtttgattt cgcaggaaaa accaatcctt caaacatgat attacgatat tttcaattcc
     5101 cagaaaatct tacaggaccc tgttatttta cattaaaact ctgggataaa attttgaatg
     5161 aaaaaagatt tgttggtgtt gcaggacttg atatccatcc tttgaaatac gctttcaggt
     5221 accttgatat caaagaaaga tttagctatg gttttgtttt caaaacattg agaaatcata
     5281 tccttgttga tgaggaattt actggcgaca tttcaaagga cagaaatatt gtgttagagg
     5341 cattgaaaaa aggacggctt ttttttgcaa atgatttact cgcagacagc aggggttttt
     5401 tctttggttc agaagatcaa acaaggacga tgggtgattg catttcgaca ggtgaaaggt
     5461 tgattattga ggtgccagaa gaagtagaca tcagaattaa aaatagggtt gagacaatct
     5521 gttttgaaaa gacgaaaaaa gcagtgatta aagcagccat tgaagggttt tacagggtgg
     5581 aggtatacta tagagaaaga ccatggattt tttcaaatcc agtgtatgta aaaggagtta
     5641 gaccatgaaa gattttccaa ggacaaatat aggtggaatt tcagtttcac ggttggttat
     5701 tgggacgaac tggttgttag ggttcagcca cacatcaaaa gcaaaggata atttcatcag
     5761 ggaaaacata ggtataagaa aaaaaatggc agacattttc gaagtgtttc tgaatcatgg
     5821 gat
//
LOCUS       DAORYU010000076         5758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_9563_length_5758_cov_6.528318, whole genome shotgun sequence.
ACCESSION   DAORYU010000076 DAORYU010000000
VERSION     DAORYU010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5758
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9563_length_5758_cov_6.528318"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1475)
                     /locus_tag="PLW07_03550"
     CDS             complement(<1..1475)
                     /locus_tag="PLW07_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012992322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOL49631.1"
                     /translation="MTKEEKFYKALQDVFIGAKIEGTGGFVNLMKIKSNYYRKIEDIL
                     KEDIESALKSYPSFRDELFDKLYSFFSRYFTESGSIYFNSTPFHNNIYEKVYTDEKDV
                     ILFWKTQMLYYVKTDRIFRSLEIGSDGFKFFFDVAKLEHKKANEKRSLVYKLDKVRED
                     ETIVFTVLYSEKGTKTKTDEILKDIKRKGIAITEEQLERAFRVFEKQSEVDFFINKNA
                     KAFLQEQFKLWSYQYFWEETPRQTSFATPLQEGNRWTADRVNQLQILKDIAFKIIDFI
                     SQFEDELVKIWNKPKFVKNSNYVITLDRIDEIELIKKILHHKNIKHQIKEWQELGIVD
                     ENFKVEDVFENSASVEGCQEFPSLRGEFKHLPIDTKYFKDLELEILGLFDDLDKSLDG
                     WLIKSENYQALNTILPKFKEKVQTIYIDPPFNLDSSDQFLYRTNYKDANWATLLENRL
                     KLAKDWLNEKGSIFVRCDHNGNWIVRPLMNDIFGKENFRNE"
     gene            complement(1864..3060)
                     /locus_tag="PLW07_03555"
     CDS             complement(1864..3060)
                     /locus_tag="PLW07_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015043044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HOL49632.1"
                     /translation="MEIKVNKEKCTGCKICVDVCPVGAIEIDSDGKAFITEKCTLCRL
                     CVKECPVGAIEIIGEEKKKADIDLSQYKGIWFFAEQKNGKLSHVAYELLGISLKLAET
                     LKEEVCGVIFTSNGKEMAQELFNRGADKAYLVEEGNLDEFRQDIYVCLMTELIKKYKP
                     NIVLASATMIGRSFIPQTAARIKTGLTADCTDLAIDPETKLLQQIRPTYGGNLMAKII
                     CESHRPQMATIRPKAVEEAKVIGNREGTIIKEDFASFNAVCRVDLISKEAVEQTIDLQ
                     EVEIIISGGRGLGDGKNFEMIRKFASLIGGAVGASRAAVDSGWIPYPHQVGQTGKTVK
                     PKIYIACGISGAIQHLAGMSTSDYIIAINKDPDAPIFKVANLGIVGDIFEVIPKLIQK
                     LESSKQ"
     gene            complement(3060..3845)
                     /locus_tag="PLW07_03560"
     CDS             complement(3060..3845)
                     /locus_tag="PLW07_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOL49633.1"
                     /translation="MKYIVCIKQVPDTMDVKVDPETKRIVREGVPSIMNPYDYYAVEE
                     ALRLKERFGGTTVAISMGPLQAEAVLREAISLGIDEAYLLSDRAFAGSDTLATSYTLS
                     QAIKKIGDFSIILCGREAMDGSTGQVGPELAEHLEIPIITYVSKIVNVSENQLECVRL
                     LEDHYESLKSPLPAVITVVKEINEPRLPSLKGMLKAKKATVPVWTKSDIGGNDAQYGQ
                     DGSPTKVVEIWRPEIKKEGKVISGEPDELAEAIYQELKKLGAA"
     gene            complement(3858..4130)
                     /locus_tag="PLW07_03565"
     CDS             complement(3858..4130)
                     /locus_tag="PLW07_03565"
                     /inference="COORDINATES: protein motif:HMM:NF013346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HOL49634.1"
                     /translation="MNWQIFWIAFSTIFLAEIGDKTQLVCFSLSTKSTSIVPVFLGAM
                     IAFVLSTFIACMLGNYLQKVVPVNVIHSIAGVILIVSGILVLINKL"
     gene            complement(4127..5479)
                     /locus_tag="PLW07_03570"
     CDS             complement(4127..5479)
                     /locus_tag="PLW07_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOL49635.1"
                     /translation="MKPDRVVFREYDIRGIVKTQINNEFAFTLGRAFGTKIKNEGLKN
                     CVVACDNRETSPDLKNHLIDGMMKAGIEVFDAGMIPTPVAYFAQKHYGLDACAMVTAS
                     HNPPQFNGFKLVLGETGLHGEQIQQIADIIEKQDFISGSGSTKTIQVEQDYIDFMKER
                     FSFSSKMKIGVDTGNGTTGPFVKKLFSSLGIDFVGLYLESDSSFPHHLPDPVVPENLV
                     DLIKLVKEQKLDAGFGFDGDGDRIGVIGSDGSILWGDQLLIIYGLDILKTKPGSTIVF
                     DVKCTLALEEEIKKAGGIPLMWKTGHSLIKAKLMEEKAVLGGELSGHIYFADQYFGFD
                     DAFYACLRLLRIMDQTGKKPEQLLENKKRYYSSPEIRIEVGEEKKYAIVEELKNFYKQ
                     KYNVSEIDGVKVYFPDGWALARVSNTQPAIVLRVEGKDEKSLEEIKKQFLSKVEETIK
                     "
BASE COUNT         1602 a         1204 c          985 g         1967 t
ORIGIN      
        1 atttcatttc taaaattttc ctttccgaaa atgtcgttca ttagtggtct tacaatccaa
       61 tttccattat gatcacacct tacaaaaata cttccttttt cattcagcca atcttttgcg
      121 agttttagtc ggttttccaa tagtgttgcc caattagcgt ctttgtaatt tgtgcggtaa
      181 aggaactggt ctgaagaatc taaattaaac ggtggatcaa tataaattgt ctgtactttt
      241 tctttgaatt ttggcaggat tgtgtttaat gcctgatagt tctcgctctt tatcagccag
      301 ccgtctaaag acttatctaa gtcatcaaac aatcctaaaa tctcaagttc taaatcctta
      361 aagtactttg tatcaatcgg aaggtgttta aattcccctc ttagagaggg gaattcctga
      421 cacccctcca cagaagcgga attttcaaaa acatcttcta ctttaaagtt ttcatctaca
      481 atgcctaatt cctgccattc tttgatttgg tgcttgatgt ttttatggtg aagtatcttt
      541 ttaattaatt caatctcgtc aattctgtcc agcgtaatca cataatttga atttttcaca
      601 aactttggct tgttccatat ctttaccagt tcgtcttcaa actgcgagat aaaatcaata
      661 attttgaaag caatgtcttt gagaatttga agttgattaa ctctgtctgc tgtccatcta
      721 ttcccctctt gtagaggggt ggcaaacgag gtttgacggg gtgtttcttc ccagaaatat
      781 tgataactcc agagtttaaa ctgctcctgc aaaaatgctt ttgcattctt gttgataaag
      841 aaatctactt cactttgttt ttcaaaaact ctaaaagctc gttctaattg ttcttctgta
      901 atcgcaatcc cttttctttt aatgtcttta agtatctcat cagtttttgt ctttgtgcct
      961 ttttcggaat atagaacagt aaaaacaatt gtttcgtctt ctcgaacttt atctaactta
     1021 taaacgagtg aacgcttttc attggctttt ttatgttcaa gtttcgcaac atcaaagaaa
     1081 aacttaaaac cgtcagatcc tatttccaaa ctcctgaaaa ttctgtcggt tttaacataa
     1141 taaagcatct gcgtcttcca gaatagaatg acatcttttt catcagtata aactttttca
     1201 tagatattat tgtgaaatgg agttgaatta aagtaaatgg agccactttc tgtaaagtat
     1261 cggctgaaaa aagaatagag tttatcaaaa agttcgtccc tgaatgaagg gtagcttttt
     1321 agtgcagatt caatgtcttc ttttagaata tcttcaatct tgcggtagta atttgatttg
     1381 atcttcatca agttaacaaa accaccagta ccttcaatct ttgcgccaat aaatacatcc
     1441 tgcagtgcct tataaaattt ttcttctttg gtcatattta ccacctcgtt attgttttta
     1501 ttataaatct ttttgtcttt aagtccataa aaaagccact tatcaaaaga aacaattttg
     1561 ctatgatttt gcttgatgaa ggttgggttt ctaattattt tttctctttt tttaagacag
     1621 caatatcata ataattgacg gtcttattta cataaaggat tggggttcaa ctttcgcgtg
     1681 tcacggcttt tatctgattt aacaaattaa aatcatcctt ttatttccag agaaggttat
     1741 ctgcctcgct ttgatattca aaggtcgttg gtgaaatata gatgttgtgc tctggatatc
     1801 caatcttcct tcacacctga aaacctattg cctgggtttt tctatcacgc agaaaatgat
     1861 tgattattgt tttgatgact caagtttttg aatgagtttt ggaattactt caaagatgtc
     1921 gccgactatt cccaggtttg ctactttgaa aataggagca tcagggtctt tgttgatcgc
     1981 aataatgtag tcagatgttg acattccagc gagatgctga attgctcctg aaattccgca
     2041 ggcaatataa atttttggtt tgactgtttt tcctgtctgt ccgacctgat gggggtatgg
     2101 aatccagcca gaatcaacag cagcacgaga agcaccaact gctccaccaa tcagtgaggc
     2161 aaattttctt atcatttcaa aattcttgcc atctccaaga cctctacctc ctgaaatgat
     2221 aatttctact tcctgaaggt caatcgtctg ctcgactgcc tcttttgaaa taaggtcaac
     2281 ccggcatact gcattgaatg aagcaaaatc ttctttaatg attgttcctt ctctgttgcc
     2341 aatcaccttt gcttcttcaa ctgcctttgg ccttattgtt gccatctgag gtctgtggct
     2401 ttcgcagatg atttttgcca tcagatttcc accataggtt ggtcgtatct gctggaggag
     2461 ttttgtttca gggtctattg caaggtctgt gcaatcagca gtaagccctg tttttattct
     2521 tgctgctgtt tgtggaataa aacttcttcc aatcattgtg gcagaagcaa gaacgatgtt
     2581 tggcttgtac ttttttatca attctgtcat caaacacaca taaatatcct gcctgaattc
     2641 atcaagattt ccttcttcaa caagatacgc cttatcagcg ccacgattga aaagttcctg
     2701 cgccatctct ttgccatttg aagtgaatat gacgccacac acttcttctt ttaatgtttc
     2761 tgcaagtttc agtgaaatac ccagcagttc atacgcaaca tgagagagtt ttccgttttt
     2821 ctgttcagca aaaaaccata ttcctttata ctgacttaaa tcaatatctg cttttttctt
     2881 ttcttcacca ataatttcaa tggctccaac cgggcattcc tttacacaca acctgcagag
     2941 agtacatttt tcagtaataa atgccttgcc atcgctgtca atttcaatgg ctccaacagg
     3001 acacacatca acacaaattt tacaaccagt acacttttct ttgttaactt tgatttccat
     3061 tatgctgctc ccagtttttt cagttcctga taaatggctt ctgctagttc atctggctca
     3121 cctgaaatga cttttccttc tttttttatt tcaggcctcc atatctcaac aacctttgtc
     3181 ggagaaccat cctgaccgta ttgagcatca ttgccgccaa tatcactttt cgtccagaca
     3241 ggaactgtgg ctttttttgc ctttaacatc cctttcaaag aaggcaatct tggctcattg
     3301 atttctttaa ccactgtaat aacagcagga agtggagatt ttaaagattc ataatggtct
     3361 tcaagcaacc gcacacactc caattgattt tcagagacat tcacaatttt actgacatag
     3421 gtaataattg ggatttcaag atgttcagca agttctggtc caacctgtcc tgtgcttccg
     3481 tccattgctt ctcttccaca taaaattatt gaaaaatctc caatcttttt gattgcctga
     3541 ctcaaagtat aagatgtggc aagtgtgtcg ctacctgcaa atgctctgtc gcttaataga
     3601 taggcctcgt caatgccaag tgatattgct tcccttagca ctgcctctgc ctgcagaggt
     3661 cccattgaga ttgcgacagt tgtgccgcca aatcgttctt tcaatcttaa tgcttcttca
     3721 acagcatagt aatcgtatgg attcataata ctgggcacgc cttcccgcac aattcttttt
     3781 gtttcagggt caacctttac atccattgtg tcaggaacct gtttgatgca cacgatgtat
     3841 ttcatgtttc ctccggatta aagtttattg atgagcacca ggataccgga tacaatcaga
     3901 ataactccgg caatagagtg aatcacgttg actggaacaa ccttttgcag gtagtttccc
     3961 agcatacagg caatgaatgt gcttaacacg aatgcaatca ttgctccaag aaaaacagga
     4021 acaattgagg tacttttggt actcaaggaa aaacagacca gttgagtttt gtctccgatt
     4081 tcagcaagga aaattgttga aaaagcaatc cagaatattt gccagttcat tttattgtct
     4141 cctcaacttt cgaaagaaat tgttttttga tttcttccag ggatttttca tctttgcctt
     4201 caacgcgaag aacaatggct ggctgcgtat ttgaaaccct tgcaagagcc cagccatcag
     4261 ggaaatagac ctttactcca tcaatctctg aaacattgta tttctgctta taaaaatttt
     4321 tcagttcttc aacgatggca tacttttttt cttctccaac ttcaattcgt atttcaggac
     4381 ttgaataata tcttttcttg ttttcaagaa gttgctctgg cttttttcct gtttggtcca
     4441 taattcttag cagccgcaag caggcataaa aggcgtcgtc aaatccaaaa tactggtcag
     4501 caaaataaat atgacctgaa agttctccac caagtactgc tttttcttcc atcaactttg
     4561 ctttgataag ggaatgacct gttttccaca tcagggggat tccacctgct tttttgattt
     4621 cttcttcaag ggcaagcgtg catttcacat caaatacaat tgttgaaccc ggttttgttt
     4681 tcaaaatatc aagcccgtaa ataataagaa gctggtctcc ccagagaatt gagccatcgc
     4741 ttcctattac gccgattctg tctccatctc catcaaaacc aaatcctgca tcaagttttt
     4801 gttctttcac aagttttatc aaatcaacaa ggttttcagg taccacaggg tctggaaggt
     4861 ggtggggaaa actgctatcg ctttcgagat atagcccgac aaaatcaata ccgagagatg
     4921 aaaaaagttt ttttacaaat ggacctgtag tgccattacc agtatcaacg cctattttca
     4981 tttttgagga aaaactgaac cgttccttca tgaaatcaat gtagtcctgt tcaacctgta
     5041 ttgtttttgt gcttccagaa ccagatataa aatcctgttt ttcaataata tctgctatct
     5101 gctgaatctg ttctccatga agacctgttt ctccaagaac aagtttgaaa ccattaaatt
     5161 ggggagggtt gtggctggct gttaccatgg cgcaggcatc cagaccataa tgtttctgag
     5221 caaaataggc aactggcgtt ggaatcatac cagcatcaaa cacttcaatg cctgctttca
     5281 tcatgccatc gatgagatgg tttttcaaat caggtgatgt ttccctgttg tcgcaggcga
     5341 caacgcagtt tttcagccct tcatttttta tttttgtgcc aaaagccctg cccagagtaa
     5401 aagcaaattc attattaatc tgggttttta caattcccct aatatcgtac tcacgaaaaa
     5461 caaccctatc cggtttcata aaacttcccc ttttaggaat gattttatca ataaagtata
     5521 ccagaaaatg ccttacattt ccagaaaacc atgatttgaa agtttcataa aaaatgaaat
     5581 ccctcttgcg tctccctttt acaaagggag aatgagatac cccctcttta taaaagaggg
     5641 ggtgagggag atttcaaaat cccccttaat cccccttttg taaaggggga aaataggaga
     5701 agggattttg aaaaggggga gaacgagagg gtaaaggaag tctgggattg attttatg
//
LOCUS       DAORYU010000077         5742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_9599_length_5742_cov_3.729383, whole genome shotgun sequence.
ACCESSION   DAORYU010000077 DAORYU010000000
VERSION     DAORYU010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5742
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9599_length_5742_cov_3.729383"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1291)
                     /locus_tag="PLW07_03575"
     CDS             complement(<1..1291)
                     /locus_tag="PLW07_03575"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOL49636.1"
                     /translation="MRFTLMVLCIMLCAGMSFAKSVTFVENGNPKAVILISSQPDPDE
                     NLAAKELVEHIEKISGCRIDIVQQGADKRWQINPALGSKGICYIFLGSVAMTPEMEKS
                     IRSKGTDPDSFMIQVQSNPAGISSIRIAGLSSEGTLFGVYDLLEQIGVRWYMPGDIGA
                     VIPEKKTITIEEQNNIQVPSFPARHLQMAADKTWYRRMRLGGPYFPSAHGINIGKPDI
                     NKEPELFALVNGKRQASQLCISNPEVLARAIKETKNYFRKNPSAEWIGMGPNDGGGFC
                     ECENCRALDAGDFDIFEHRVSMTDRYIWFFNEVLKGISDEFPDKKIGFYCYSCYMKPP
                     VKVKPDPRIVPAFAPISLCRIHGMSNPVCPERSYYRKIMEAWGKILPETYERGYFFNL
                     ADPGFPFSKVHAIRDEIPVAHQLGVKGWRVETIPHWAS"
     gene            complement(1288..2181)
                     /locus_tag="PLW07_03580"
     CDS             complement(1288..2181)
                     /locus_tag="PLW07_03580"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOL49637.1"
                     /translation="MYFTGFADEAAPDIDGQIRATKELGWKYIESRSIDGVNLTMVDD
                     RKFDEVVEKLQQAGIKINCFGSGVANWAQKITEPPDKSYEEMKRAIPRMHRLGTKMIR
                     IMSFAIPPEELDKDWSKEAINRVKTIVKMAEDGGVLCVHENCSGWGSLSWEHTLRLLE
                     GVNSPALKLVYDTGNPLFHQDVRGPKPYKMQDPWEFYTHVKQHIVYVHIKDGYMTEDG
                     KHVCTFPGEGNGMVREILTDLFDSGYDGGISIEPHMAVVYHDPSVTSEAEVRYRNYIE
                     YGRRIEKLVSEILNQLNEAKK"
     gene            complement(2195..3478)
                     /gene="rmuC"
                     /locus_tag="PLW07_03585"
     CDS             complement(2195..3478)
                     /gene="rmuC"
                     /locus_tag="PLW07_03585"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOL49638.1"
                     /translation="MIFIFSLIVVFAIYLLHVRESSKSLREKDARIAELEANIQALSD
                     QNSKLMIEIAELKKEKETYIEKISWTEKSQEQLKDAFNSIATQALRGNAEDFLKVARQ
                     SLESILQQVRGDWGTHRVELQKLVEPLSETLKKMDTEIRELEKKREGAYRGLQEQLQH
                     LFTTQDQLKMLTVQLNQALRTTEVRGQWGQIQLRRVVELAGMTKHVDYEEQVSSQEGR
                     PDMIIHLPNGGILPVDAKSPMVAYLDAVNSEPAQKQEKLRSFARKVRETIQNLSSKKY
                     WAQFNPSPEVVIMFVPNEASITAAYESDPGLLEYAAENHVLIATPVTLLGLLKAVAFG
                     WQQYSIVENARFIAEIGSQLYERMTVLVEHLRSTGKALDTTVQNYNATIGSLETRLIP
                     SMKKLQDLGVSAKEIEPPSSVEKSLHLPKIEQDNP"
     gene            complement(3542..4906)
                     /locus_tag="PLW07_03590"
     CDS             complement(3542..4906)
                     /locus_tag="PLW07_03590"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOL49639.1"
                     /translation="MISLRSFRARIFFVFVLPVLIVIITGNAFLCWYMLKHQRKLLKT
                     ELLSIAVTCAATLDTGQITAIFDHSYVSSVSLERTLKKIINDIPSVSSICILTRQGNG
                     NFLVVAGANSDAGTAFLKGNSYPVDKFPGILMGFAVPFVSEKNNRSGFLTGYVPLRDA
                     SGTPVAVLKVDYNVRNMEGIKHTIYLQTIGTSIILIALVVLFGFFISHQISVPVNALV
                     EGIKNIQRGNFEYRISLRTNDEFGQLARIFNQMTTGLVASRKMLQNYLYRTIRSFVTI
                     LEARDSYTKGHSERVASISEKIAIKMGLPEKKVKILRETALLHDIGKLGIGEHILQKT
                     EPLTEEEWKIIKTHPVVGEEILRPVFFEKDAMEIVKQHHERFDGKGYPDGLDKNDINL
                     LARILSVADAFDAMTSARAYRKPLSIPEAMKELKANSGTQFDPDIVNTFLKILEEEMH
                     ELVG"
     gene            complement(4953..5549)
                     /locus_tag="PLW07_03595"
     CDS             complement(4953..5549)
                     /locus_tag="PLW07_03595"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOL49640.1"
                     /translation="MSALDLRSVRDFVVRVENREHTAKLIGTGKQGEIFVELTDQSAG
                     ESGMGKNGTAFFLWEGKWHYFTCKIFCPSLRRMAIVRTSPIAIDSRKERRYETGLLSG
                     RAVEKGLMHGAIDVNILNVSRTGIRIETRQPLKTGKTYLVEMKLVIKHFSEIVRAECV
                     VMNETRVGSMYHHGCQIVDISPSDAILLEKYLGIVAMP"
BASE COUNT         1560 a         1332 c          995 g         1855 t
ORIGIN      
        1 tgcttgccca atgcggaatt gtctcaaccc tccatccctt tactcctaac tggtgggcaa
       61 caggaatttc atctctgatt gcatgaactt tactgaatgg aaaaccaggg tcagcaagat
      121 tgaaaaagta acccctttcg tatgtttcag gaagtatttt cccccatgct tccataattt
      181 ttctgtagta acttctttcc ggacatacag gatttgacat accgtgaatc ctgcacaaac
      241 tgattggcgc aaaagcaggt acaatccgcg ggtcaggttt aaccttcacc ggtggtttca
      301 tgtagcagga gtaacagtaa aacccgattt tcttatcagg aaattcatct gaaatacctt
      361 taagcacttc attgaaaaac cagatgtatc tgtcagtcat tgaaaccctg tgttcaaaaa
      421 tgtcaaaatc ccctgcgtca agggctcgac aattttcaca ctcacaaaat ccacccccat
      481 cattgggtcc cataccaatc cactcagcag atggattctt cctgaagtaa ttctttgttt
      541 ctttaattgc cctcgcaagt acctcaggat ttgatataca caactggctc gcctgccgtt
      601 tcccattcac aagagcaaaa agttctggct ctttattgat gtctggcttt ccaatgttaa
      661 tgccgtgggc tgatggaaaa tatggtccgc caagtcgcat ccttctatac caggttttat
      721 cagctgccat ctgcagatgt cttgcaggaa aagatggaac ctgaatatta ttctgctcct
      781 caatggttat ggttttcttt tctggaatca ctgcgcctat gtcacctggc atataccatc
      841 tcactccgat ttgttcaaga aggtcatata caccaaatag tgtcccttca ctggataacc
      901 ctgcaatcct gatactgctg ataccagcag gatttgattg aacctgaatc ataaatgagt
      961 cggggtcagt gcctttagaa cgaatggact tttccatctc aggagtcatg gcaacactac
     1021 caagaaaaat atagcatatc cctttgctgc caagtgcagg atttatctgc catctcttat
     1081 ctgcaccctg ctgaacaatg tcaattctac aaccactgat tttctcaatg tgctcgacaa
     1141 gttcctttgc tgcaagattt tcgtctggat caggttgaga agaaatcaga atcactgctt
     1201 taggatttcc gttttcaaca aatgtgactg attttgcaaa agacatgcca gcacacaaca
     1261 taatacacag caccattaat gtaaatctca ttttttcgcc tcattcagtt gattcaaaat
     1321 ttcagaaaca agtttttcta tccttcttcc atactcaata taatttctgt accttacttc
     1381 tgcttctgat gttacgctcg ggtcatggta cacgacagcc atatgcggtt caattgatat
     1441 tcctccatca tagccactgt cgaaaaggtc agtaagaatt tctcttacca tgccatttcc
     1501 ttcccctgga aatgtgcaaa catgtttccc atcttctgtc atatatccat ccttaatatg
     1561 gacgtacact atatgctgtt ttacatgggt ataaaattcc catgggtcct gcattttata
     1621 aggttttgga cccctgacat cttgatggaa aaggggatta cctgtatcat aaacaagttt
     1681 taatgcaggg gagttgactc cttcaaggag acgaagggta tgttcccagg aaagagaacc
     1741 ccatcctgaa cagttttcat gcacgcataa aacacctcca tcttcagcca tcttcacaat
     1801 ggtcttcacc ctatttattg cttcttttga ccaatcctta tcaagttctt ctggcggaat
     1861 ggcaaaactc atgatacgaa tcatttttgt gccgagtcga tgcattcgcg gaattgctct
     1921 tttcatctcc tcatagctct tgtctggtgg ttctgtaatc ttttgagccc agtttgcaac
     1981 accactgcca aaacaattaa tttttattcc tgcctgttga agtttttcaa ccacctcatc
     2041 aaattttctg tcgtcaacca tcgtgagatt gactccgtca atgcttcttg attcaatata
     2101 cttccagcca agttcttttg tcgcgcgaat ctgtccatca atatcaggcg ctgcttcatc
     2161 agcaaatcca gtaaaataca ttcttcctcc ctgtttatgg attatcctgt tcaatttttg
     2221 gtaagtgcaa cgatttttct acactactgg gtggttcaat ttcttttgca gacacaccaa
     2281 ggtcctgaag ttttttcatt gacggtatca gcctggtttc gagagaacca atagtcgcat
     2341 tgtaattttg cacagttgta tcaagtgctt tacctgtgct cctgaggtgt tctaccagca
     2401 ctgtcattct ttcgtacagt tgactgccta tttcagcgat aaaccgggca ttttctacaa
     2461 ttgagtattg ctgccatcca aaagcaactg cctttaacaa tcccagaagt gttacaggcg
     2521 ttgcaataag cacatgattc tctgctgcat attccagcaa tcccgggtcg ctctcatatg
     2581 cggcagtaat cgacgcttca tttggtacaa acataatcac cacctctgga gaaggattaa
     2641 attgagccca gtatttcttg cttgaaagat tttggattgt ctcacgcacc ttccttgcaa
     2701 aagaacgtag tttttcctgc ttctgggctg gctctgaatt tactgcatca agatatgcta
     2761 ccattggact tttggcatca acaggcaaga ttccaccatt tggaagatgg ataatcatat
     2821 cgggtctacc ttcctgactg ctcacctgtt cttcgtaatc aacatgtttt gtcataccag
     2881 caagttcaac cactcttctc aattgaatct gtccccactg gccccgcacc tctgtggttc
     2941 tcaatgcctg gtttaattgt actgtgagca tttttaactg atcctgtgtt gtgaacagat
     3001 gttgaagttg ttcctgtaat cccctgtaag ccccttccct tttcttctca agttctctta
     3061 tttcggtatc catctttttc aatgtttctg aaagtggttc aacaagtttc tgcaattcca
     3121 ccctgtgagt gccccaatca ccccttacct gttgaagaat ggactcaaga gattgtctgg
     3181 ctacttttag aaaatcttct gcattacccc tgagtgcctg ggtcgcaatg gaattaaatg
     3241 cgtctttcaa ttgttcctgg gatttttctg tccatgaaat tttttcaatg tatgtctctt
     3301 tttctttttt caattccgct atttcgatca tcagttttga attctggtct gatagcgcct
     3361 gtatattggc ttcaagttca gcaatccgtg catccttttc tctcagtgat tttgaactct
     3421 cccggacatg tagcagatat attgcgaaaa ccacaattaa cgaaaaaata aatatcatcg
     3481 tgttcacgtt taaccccaaa atttattata caactttttt cgcttgcttt cacacatatg
     3541 gtcaaccaac gagttcgtgc atttcttctt caagtatttt cagaaatgta ttgacaatgt
     3601 ccggatcaaa ttgtgttcca ctattggctt ttaattcctt cattgcttcc ggtattgata
     3661 gtggctttct ataagccctg gctgaagtca ttgcgtcaaa tgcatctgca acagacaata
     3721 tccgggcaag cagattgata tcatttttgt ccagtccgtc agggtaccct ttaccatcaa
     3781 acctttcatg gtgttgttta acaatttcca tagcatcttt ttcaaaaaat acaggtctca
     3841 gaatttcttc accgacgaca ggatgcgttt taataatctt ccactcttct tcagtaaggg
     3901 gttctgtctt ctgcaatata tgttccccta taccaagttt gccgatatca tgcaacaaag
     3961 cagtttcccg aagtattttt accttctttt caggaagccc cattttaatt gcgatctttt
     4021 cagaaataga agcaaccctt tctgaatgac ctttcgtata agaatccctg gcttcaagaa
     4081 ttgtgacaaa cgaacgtatt gttctgtaaa ggtagttttg aagcattttc cgtgatgcca
     4141 ccagtcctgt ggtcatctgg ttgaatatac gggcaagttg cccgaactca tcattagttc
     4201 tcaatgaaat gcgatattca aaattccctc tctggatatt ttttattccc tccacaagcg
     4261 cattcacagg cacagaaatc tggtgtgaaa taaaaaaacc aaatagcacc acaagagcaa
     4321 ttaatattat tgacgttcca attgtctgta agtaaattgt atgcttaatt ccttccatat
     4381 ttctgacatt ataatccacc tttaatacag cgacaggtgt tccgctggcg tccctcaaag
     4441 gtacatatcc tgtcaaaaaa ccggacctgt tatttttttc tgaaacaaaa ggaacagcaa
     4501 aacccatcaa aatcccggga aatttatcaa ccgggtaaga attccctttt aaaaacgcgg
     4561 tacctgcgtc tgaatttgct cccgcaacaa caagaaaatt accattgccc tgtctggtga
     4621 gaatgcaaat ggaagaaacc gaaggaatgt cgttgatgat ttttttcagt gtgcgttcaa
     4681 gggaaacaga agaaacataa gaatgatcaa aaattgcggt tatctgtcca gtatccagtg
     4741 ttgcagcaca ggtcacagcg atagacaaaa gttctgtttt cagtaatttc ctttgatgct
     4801 tcaacatgta ccagcacagg aaagcatttc ctgttataat cacaattaaa acaggcagta
     4861 caaatacaaa aaaaatcctt gccctgaaac ttcttaacga tatcattttg cgaattgaat
     4921 aataaaaatc ccctgctttt gattttgaat actcacggca tggctactat accaagatat
     4981 ttttcaagca gtatcgcatc agacggtgaa atatcaacaa tctggcaacc atgatgatac
     5041 attgaaccca ccctggtttc attcattaca acacattcag ccctgacaat ttcagaaaaa
     5101 tgttttatca cgagtttcat ttctaccaga taggtttttc ctgtcttcaa tggttgtctg
     5161 gtttcgatgc gaatacctgt tctgctgaca ttgagaatgt tcacatcaat ggcaccatgc
     5221 atcaatcctt tttcaactgc tctcccactt aaaagtcctg tttcatagcg cctttcctta
     5281 cggctatcaa tggcaattgg agaagttctg actattgcca ttcttcgcaa acttggacag
     5341 aaaattttgc aggtaaaata gtgccacttt ccttcccata agaaaaatgc agtaccattt
     5401 ttgcccatac cactttcacc ggcggactgg tcggttaatt caacaaaaat ctcgccctgt
     5461 tttcctgtgc caatcagttt tgccgtatgt tcccggttct ccacccttac gacaaaatca
     5521 cgcacacttc tcaggtcaag tgctgacatg gtccccacaa ttactttacc tttttaataa
     5581 aatttagtca acataaaaga aaagactgat ttgtgttata attttttgat tgccaggaga
     5641 tatctatgtt tttgcagctt ttcgtaaggg tattgtccat tttttttatc atatttgttg
     5701 gtgcttttgc aagaaaaaag aagtttctaa atgatgaaac aa
//
LOCUS       DAORYU010000078         5705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_9694_length_5705_cov_3.625133, whole genome shotgun sequence.
ACCESSION   DAORYU010000078 DAORYU010000000
VERSION     DAORYU010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5705
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9694_length_5705_cov_3.625133"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..314
                     /gene="rpmH"
                     /locus_tag="PLW07_03600"
     CDS             180..314
                     /gene="rpmH"
                     /locus_tag="PLW07_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOL49641.1"
                     /translation="MSITLRPNKRKRKKVHGFRKRMSTHSGKKVLSRRRRKGRKELTA
                     "
     gene            323..595
                     /gene="rnpA"
                     /locus_tag="PLW07_03605"
     CDS             323..595
                     /gene="rnpA"
                     /locus_tag="PLW07_03605"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOL49642.1"
                     /translation="MLDVIKTGKKYETEHFIFYVKHTENLRIGFRFSSTFAKAVTRNR
                     IKRIFRKLIRENVQSGDLVIRAKGKSKILTQKDVEQEWKSIAGKIL"
     gene            592..816
                     /gene="yidD"
                     /locus_tag="PLW07_03610"
     CDS             592..816
                     /gene="yidD"
                     /locus_tag="PLW07_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003106504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOL49643.1"
                     /translation="MTEVLVFIVKIYQVIISPVFGHNCRFYPSCSDYLIEALRHTGIR
                     RGLFLFAKRIIRCHPFNPGGYDPIPGRNSQ"
     gene            813..2339
                     /locus_tag="PLW07_03615"
     CDS             813..2339
                     /locus_tag="PLW07_03615"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family insertase periplasmic-domain
                     containing protein"
                     /protein_id="HOL49644.1"
                     /translation="MKEETRIMIAFALALLVIILFSITPKKQPVQVKKESVPAPVSAP
                     AVEKQNQSGIPAEFEVSNNQIKFENQLYKGAIDLLGGKIVSFSLKNYIRPGEKTFDFF
                     QKGNTLLDTVEGETQQLVYRLSPESKDGFYVLQARRNDIEFEKSIKIAPDRYLWNVEI
                     QQKNLANKTAISPETNIILGSVTLNRKHPEDASIDFLVMNGGKPYKFNVARQMKEKIS
                     GNVCVVRTRYQMYYFKVDTPVEFNVENVASKVVWGFKVPSSQFSTGQTKKISGDFYIG
                     PSDYFVARSEISDMRIFGTGFFVSMGRLIFHVLNSIHRVIPNWGLAIIILTILIKIIF
                     FPLTRSSLKSMKQLQKLRPYLQDVQKKYKDNPQMLQREMMNLYKEYKINPFGGCLPML
                     VQIPIFVGFFLALRNSIFLRGSKFIFWIKDLSLPDTIATIGSIPINILPVLMFASSFL
                     QQKMTPSVEQSQKFMNVMLPLMMLVLFYNFSSGLLLYWVTMNLAGLVEQYYIYKIAKK
                     "
     gene            complement(2342..2998)
                     /gene="rnc"
                     /locus_tag="PLW07_03620"
     CDS             complement(2342..2998)
                     /gene="rnc"
                     /locus_tag="PLW07_03620"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOL49645.1"
                     /translation="MPMQDLKEIEHKIGYVFKNSELLKTALTHSSYAHQTDEESYERF
                     EFLGDAIMSYIVALEIFKKNPGKNEQFLTELKSAYVNKNFLQKLGKELGLQHCIRKIG
                     LSEPRLDQAVEALVAAIYLDGGYYKAKRFIKKFILGKPVKPLQDYKNLLKSFVSEKFN
                     KDIRYKLEKESGPPHDRIFQISVSIPGEKYFARATGKSKKEAELKASGLLLKKIQKEG
                     "
     gene            complement(2980..4395)
                     /gene="lnt"
                     /locus_tag="PLW07_03625"
     CDS             complement(2980..4395)
                     /gene="lnt"
                     /locus_tag="PLW07_03625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOL49646.1"
                     /translation="MFSFFFAIISGFILCFAFPPFKYWFLAWFGFVPILFLMDEKSPG
                     RNFLKGFLSGFVFYVFLLNWIYSVAGFFYLLIAVYLALFWGVFFSLVFTFPERTRILI
                     GACLWYFFEIVVRHLISGFPWIFFSLSQWFFPKTGCISAIIGSNGLSGLIIAANFSLF
                     WMIKYKKFYPVSVTAVIVIILYFAATFNISQEKRDTFIKIAAIQGNSGYFGQNPEESF
                     ENYKELTESLKSKYDLVVWPESSYPAILQDNAEAFNYLVNKSRSFPLLVGTMSEEKTN
                     LYNSAYYFNDGKAVKYSKRHLVPFGEYVPGKSFQIIKKIYSQFSDIFPEVTPGDTSSI
                     FHLSTCKFSVLICFENIFPEIALQDVKNHPDFTVVITNDSWYGNSFGPYQHFAHNIFR
                     SYETGRSIVQVSTTGITGLVSPSGDVKIYQKNEKNLFVDGIMTILVPCTRKMKTFYST
                     VGDVGIFVVFLLITGVCLCRI"
     gene            complement(4426..5376)
                     /locus_tag="PLW07_03630"
     CDS             complement(4426..5376)
                     /locus_tag="PLW07_03630"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HOL49647.1"
                     /translation="MTDVLTIGETMIRLSTVNYQTVEQSGNLAFSIAGSESSVAISLS
                     RLGIKTIWISKLVNNFLGRKIVQEIQKHGVDTSKVIWTDIGRVGVYFIEYGSVPRPIK
                     ILYDRKNSAINSLRPDEIDWNCLNNIKLIHLSGITPALSKNCAKLTRTAINEAKGRKK
                     IISFDLNYRTKLWSPYQARKFFEEVLPDVNILIAARKDIEVIFAMKEEPDRMIEKIKR
                     RFKNDVVVLTAGSEGAFADDGKLYFHKAPEVKVIDRIGAGDAFTAGFIYGYLKKDVEL
                     ALTYATTLAALKCTIPGDIPYITKQDVDGFIKSEHKDIER"
     gene            complement(5369..>5705)
                     /locus_tag="PLW07_03635"
     CDS             complement(5369..>5705)
                     /locus_tag="PLW07_03635"
                     /inference="COORDINATES: protein motif:HMM:NF013263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-dehydro-3-deoxyphosphogluconate aldolase"
                     /protein_id="HOL49648.1"
                     /translation="IPFIPGVFTPNEIVNAWQNGAKIVKLYPAVACGPEYIKVLKNGP
                     FSQIIFMAVGGITLSNAKDYIKSGADILGIGGALVNEKLVSDQNWNIVQKNARQFIEI
                     VKSARSKND"
BASE COUNT         1849 a         1012 c         1034 g         1810 t
ORIGIN      
        1 aaaagtttat tactggctta ccggtgaatt tatggaagaa actgatattg gggaatctga
       61 tacccgggct ctcaaagatg gatttatttc cattacgcca ttgactgttg atttgacaaa
      121 ttacgaatac gttaaacata tttcaaactg gaattttaac gacataatca gggggtaata
      181 tgtcaattac attaagaccg aataagcgca aaagaaaaaa ggttcatgga tttagaaaaa
      241 gaatgagtac gcattccgga aaaaaagtac tatccagaag aagaaggaaa ggacgaaaag
      301 aacttactgc atgaagaaaa gtatgcttga tgtgataaaa acaggcaaaa aatatgaaac
      361 agaacatttt attttctacg tgaaacacac agagaattta agaatcggtt ttcgttttag
      421 cagtactttt gcaaaggcag ttacgcgaaa cagaataaaa cgaattttta gaaaattaat
      481 cagggaaaat gttcaatcag gagaccttgt catcagggca aagggtaaga gtaaaatcct
      541 gacacaaaag gatgtcgaac aggaatggaa aagcattgca ggaaaaatct tataactgaa
      601 gtgttagttt ttattgtcaa aatatatcag gtaattattt cacctgtgtt tggacacaat
      661 tgcagatttt atccaagttg ttcagattac ctgattgagg cgttaagaca cacaggaata
      721 agaaggggtt tatttttgtt tgcaaaaaga atcatcagat gtcatccttt taatccgggc
      781 ggatatgacc caataccagg gagaaattct caatgaaaga agaaacaaga attatgattg
      841 cctttgctct tgcgctgctg gttatcatct tgttcagtat cacgccaaag aaacagccag
      901 ttcaggtaaa aaaagaatct gtgcctgcgc cagtttctgc acctgctgtc gagaaacaga
      961 atcaatctgg tataccagca gagtttgagg tttcaaataa ccagattaaa tttgaaaatc
     1021 agttgtataa aggcgctatt gatttactcg gcggaaaaat cgtgtctttt tctctgaaaa
     1081 attatattcg tcctggtgaa aaaacttttg atttttttca aaaagggaat accttacttg
     1141 atactgtgga aggggaaacc caacaacttg tttatagatt atctccagaa tcaaaggacg
     1201 ggttttacgt tttacaagca aggcgaaatg acattgaatt tgaaaaaagt atcaaaattg
     1261 cgcctgatag atatctctgg aacgtggaaa tacagcagaa gaaccttgca aataaaacag
     1321 caatttctcc ggagacaaac attattctcg ggtctgttac tctaaaccgt aagcatcctg
     1381 aagatgcatc cattgatttt cttgttatga atggaggcaa gccatataag tttaatgtcg
     1441 caagacagat gaaagaaaaa atttcaggaa atgtgtgcgt ggtaagaaca aggtaccaga
     1501 tgtattattt taaggtggat acaccggttg agtttaatgt tgagaatgtt gcttcaaagg
     1561 tggtctgggg atttaaagtt ccatcctcac agttttcaac cggccagaca aaaaaaatct
     1621 ccggtgactt ttatattgga ccttctgact actttgttgc aaggagtgaa atatctgaca
     1681 tgagaatttt tggaactggt ttttttgttt ccatgggtag attgattttc catgttttga
     1741 actccataca cagggttatt ccaaattggg gattggcaat cataattctt actattttga
     1801 taaaaataat ctttttcccg cttacaagaa gtagtttgaa gtccatgaaa caactgcaaa
     1861 aactgagacc ttatcttcag gacgtacaga aaaaatacaa agataatcca cagatgctgc
     1921 aaagagaaat gatgaatctt tacaaggaat acaaaatcaa tccttttggg ggatgtcttc
     1981 caatgcttgt tcaaataccg atctttgtcg ggttttttct tgcgttaaga aactcaatat
     2041 ttttgagagg aagcaaattc attttctgga taaaagacct ttcgcttcct gatactattg
     2101 caacgatcgg gagtattcca attaatatcc tgccggtttt gatgtttgcg agttcttttt
     2161 tgcagcaaaa aatgacacct tctgttgaac aatctcagaa atttatgaac gttatgttgc
     2221 ccctgatgat gctggttctt ttttataatt tttcttctgg attgcttcta tactgggtta
     2281 ctatgaatct tgcagggctt gttgagcaat attatattta taaaattgcg aaaaagtagc
     2341 cctatccttc tttttgaatt tttttcaaca gcaaccctga cgcttttaat tcagcttctt
     2401 ttttactttt gcctgtggct ctggcaaagt atttctcccc gggtattgaa actgaaattt
     2461 gaaatattct gtcatgaggt ggccctgatt ccttctccag tttatacctg atgtctttat
     2521 tgaatttttc actgacgaaa cttttgagaa gatttttgta atcctgaaga ggttttacag
     2581 gtttaccgag tataaatttt ttgataaatc tctttgcctt gtaatatcct ccgtcaagat
     2641 aaatggctgc gacaagagct tcaacagcct ggtcaagtcg tggttcggaa agcccaattt
     2701 ttcttatgca atgttgaagt cccaattctt ttccaagttt ttgtaaaaaa ttcttattga
     2761 cataggcgga tttgagttct gtgagaaatt gttcgttttt acctggattt tttttgaata
     2821 tttcaagggc aacaatataa ctcatgattg catcaccaag aaactcgaat ctttcatatg
     2881 actcctcatc tgtttgatgc gcgtatgatg agtgggtcaa agcagtttta agaagttcag
     2941 aatttttgaa aacgtaacca attttatgtt caatttcttt caaatcctgc ataggcatac
     3001 ccctgttatt aacaaaaata ccacaaaaat tcctacatca ccaacagttg aatagaaagt
     3061 tttcatcttt cttgtacatg gtactaaaat tgtcataata ccatcaacaa aaagattttt
     3121 ctcatttttc tggtagattt taacatcacc tgacggagaa acaagtcctg taataccggt
     3181 ggtagaaacc tgaacaatgc ttcttcctgt ttcatacgac ctgaaaatat tatgagcaaa
     3241 gtgttgatat ggtccgaacg aatttccgta ccatgagtca ttggtaatga caaccgtaaa
     3301 atcaggatga ttttttacat cctgcagagc aatttcagga aatatgtttt caaagcagat
     3361 aagtactgaa aatttacatg ttgaaaggtg aaaaatgctt gaagtgtcgc cgggtgtgac
     3421 ttcaggaaaa atatctgaga attgagaata gattttttta atgatttgaa aactttttcc
     3481 tggaacatat tctccaaaag ggactaaatg tcttttgcta tattttacgg cctttccgtc
     3541 attgaaataa taagcgctgt tgtataaatt tgttttttct tcgctcattg ttcccactaa
     3601 gagtgggaaa gagcgtgatt tatttaccag ataattaaat gcttcggcgt tatcctgtag
     3661 aattgcagga taactgctct ctggccatac aacaaggtca tacttagatt tcaatgattc
     3721 ggttaattct ttataatttt caaatgactc ttcagggttc tgtccaaaat aaccactatt
     3781 accctgtatg gcggcaattt tgataaaagt atctcttttt tcctggctta tattgaatgt
     3841 ggcggcgaaa tataatatga taacaatgac agcggtgaca gaaactggat aaaacttttt
     3901 atactttatc atccagaaca acgaaaaatt tgcagcaatt attagacctg aaagtccatt
     3961 tgaaccaatg atagcagaaa tgcatcctgt ttttggaaaa aaccactgtg acagagagaa
     4021 aaaaatccag gggaaaccac tgatcaaatg acgtacaaca atttcaaaaa aataccatag
     4081 acaagcaccg ataagaattc gtgtcctttc aggaaatgtg aaaacaagag aaaaaaagac
     4141 accccagaaa agtgcaagat agaccgcaat cagtagataa aaaaaaccag cgacagaata
     4201 tatccagttc aaaagaaaca cataaaaaac aaaacctgat aagaatcctt ttaaaaaatt
     4261 cctgccaggt gacttttcat ccatcaaaaa cagaatagga acaaaaccaa accatgcaag
     4321 aaaccaatat ttgaaaggtg gaaaagcaaa acaaagaata aaccctgata ttatcgcaaa
     4381 aaagaaggaa aacatctttt tcaaccgcat gcctccatta tgacctcatc tttctatatc
     4441 cttatgttcg ctttttatga aaccatcaac atcttgcttg gtgatatatg gaatatctcc
     4501 agggatcgta cattttaatg cagccagtgt cgttgcgtac gttagagcca attccacatc
     4561 cttttttaga tacccataaa taaaacccgc agtaaatgcg tcacctgcac caatgcggtc
     4621 aatgacttta acttctggtg ctttatgaaa atacaattta ccatcatctg caaatgcacc
     4681 ttctgaaccc gcagttaata caacgacatc atttttgaat cttctcttta ttttttctat
     4741 cattctatcc ggttcttctt tcattgcgaa aattacttca atatcctttc tggcggcaat
     4801 taggatatta acatcaggca aaacctcttc aaaaaacttt ctcgcctgat acggactcca
     4861 gagttttgtt ctgtagttaa ggtcaaaaga gataattttc ttgcgccctt ttgcttcatt
     4921 gatggctgtt ctggtcaatt tcgcacaatt tttacttaat gccggggtaa tgcctgatag
     4981 atgaatcaac tttatattat taagacaatt ccagtctatt tcatcgggtc tcaatgaatt
     5041 tattgcagaa ttttttctat cataaagaat ttttattggt cttggaacac taccatattc
     5101 aataaaatac acgccaactc tgcctatgtc ggtccagata acttttgagg tatcaactcc
     5161 atgtttctgg atttcctgga caatttttcg tccgaggaag ttgtttacaa gttttgatat
     5221 ccatatggtt ttaataccga gtcgggagag agaaatagca acactggatt cagaacctgc
     5281 aattgagaag gcaaggttac ctgattgttc aacggtttga taattaactg ttgataatct
     5341 tatcatcgtt tcgccaatgg taagtacatc agtcattttt actccttgcg gatttaacta
     5401 tttcaatgaa ttgtctggcg tttttttgga caatattcca gttttggtcg cttaccaatt
     5461 tttcattgac aagtgcgcct ccgattccca gaatatcagc gccagatttg atgtaatcct
     5521 ttgcgtttga cagtgttatt ccgccgacag ccataaaaat aatctgtgaa aaaggtccat
     5581 tttttaaaac ctttatatat tcaggtccac aggccacagc gggatatagt tttacaattt
     5641 ttgcaccatt ttgccatgca ttcactattt catttggagt aaaaactcct ggaataaaag
     5701 gaatt
//
LOCUS       DAORYU010000079         5638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_9846_length_5638_cov_4.535913, whole genome shotgun sequence.
ACCESSION   DAORYU010000079 DAORYU010000000
VERSION     DAORYU010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5638
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9846_length_5638_cov_4.535913"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="PLW07_03640"
     CDS             <1..353
                     /locus_tag="PLW07_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49649.1"
                     /translation="GRGGEDLSVYNYPDYNEKVRKFAGVLLGEMLPKPEVWTDNAIVE
                     ANLLKDKKTGSYYVSLVNYNGKPVKNLKVMIDAGQIDVREVTSVFQKSKTEKNRDIVT
                     VTLDINDFDFLILK"
     gene            396..2492
                     /locus_tag="PLW07_03645"
     CDS             396..2492
                     /locus_tag="PLW07_03645"
                     /inference="COORDINATES: protein motif:HMM:NF017263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOL49650.1"
                     /translation="MTVINSFRCIGSKTLFLLTFFPYFYLFAQTIEFPRHPIFLYTDP
                     EIEEVKKDLSKNSEIQTKIKQADKIISQPLIVPEKEGDWIFYYFCPQDDAMLKYVETG
                     IHICPVCKKQFTDERTNAAYNTVINYDLDKKSVIVATAYAFTKEEKYAAWVKEYLLTL
                     AKLYPSFQRHDRWGRKGLLAVVGGRRYAQNLDEAVSAIDLASAYDLIANASCISRDDR
                     QKMEQLLRNIVREILKYQIFHGGRNNHQTWFNAAYAVVGLVTGDETLLKESIYGKYGL
                     LWQIENSITSDGLWYEGTIAYHFYALQAIQHTLDAARRAGLDFSKNEKLKSMWLGPIL
                     MAYPDGKLPVFHDSDPVELKNYRRFYLWAYRYFKDGIFAKYAGIESIDGKDIELKSTN
                     LSGIGVVSLRKTFNDNQVCVMLDYGIHGDSHGHPDKLNIVLFANGKEFLLDSGRISYS
                     VPEYKSWCRTTIAHNTVVVDEKDQQPATGTLIYFEDNDRYSACLANVDSAYPGYGLKR
                     FLILAENLLVDVFSVEGEKQSQMDWIIHCRGKVVDDFLHKKVQNLGTGNGYQHLKDIK
                     QLVSISEPVVYQCEQNDKMLKVFLVNNSGCSIFTGYGIGYHLKDNVPFLMKRVKRNCA
                     VFIAVYDFSGSVKTIKPVVVLDEKNRRMPDYQALGLSIELENKTIEIGLDLREKIAQP
                     VLFGNNKFKRIFFNVR"
     gene            complement(2479..3582)
                     /gene="mnmA"
                     /locus_tag="PLW07_03650"
     CDS             complement(2479..3582)
                     /gene="mnmA"
                     /locus_tag="PLW07_03650"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOL49651.1"
                     /translation="MKKFIMKWEIFMTRQKRVIVGMSGGIDSTVSAILLKKQGYDVTG
                     VFMMVWDKNNHVNCKMKSSCYGPEDKEIENLEKISKLTGITVHFIDLKKEFHRYVLDY
                     FKKEYLSGKTPNPCVVCNRFIKFQVLLEKVQQSGIEYDCFATGHYARVEFDGNRYILK
                     KGIDENKDQSYFLFLLTQQQLSKLIFPLGYYTKQQVREFAKQRGIEIAEQEESQDFIA
                     GDRFFLFDEGLKPGDIVDKNSRVLGKHRGIIHYTIGQRRGLGISNKKPLYVIEIKPEK
                     NVIVVGEEIDLYRDKLIAENVNFMAIESLEKPLTVQTKIRYKHRAAHSIISPIGNNRV
                     GVVFDKPQRAITPGQAAVFYDEDRIIGGGFIAH"
     gene            3639..>5638
                     /locus_tag="PLW07_03655"
     CDS             3639..>5638
                     /locus_tag="PLW07_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49652.1"
                     /translation="MKSRIWLAGLFVLIFCTMSLASDNKDIEKILGSSSENWTITGAK
                     YNVISENNIPILATARDGHISITGKQVYKAPAEYNFYFYLQPEKPYGSSITVSAGCAD
                     LPDGSKQEFTASVSAAANANYINYSFGIKPETAKAKSGHLYFQAFSDMNLSWPEEMRK
                     NIEWQIASAPKINETLHNLRLVITKTYFRVYVDGRFVGQHKVKEGIDGSGTVKLTMYY
                     GTKLVNMKIGGTDEIDERFFPLELTGFLNSSSIIKGLALAKGEIENLKERLKKEEIPF
                     YVPASQKGNDHIDLGTSWTKFGAIKGYIAANFGTFGGRWISADRIDRTRFCMYVPYSK
                     YKALHIIGTFDGEKFNVPVITAQFYRPNAGHPANFAGKVPLYSAKSGAEKIYPVKLSN
                     GQTINLFHVVIPVDPGAMDWFSDLDVVGLELTKEVKPYRAYPDPLEYSWHGAGLPSGV
                     HIYAATLEAVQTDMDIQPDAVGHVWTAPLKPSYTINIVNKTDTAKNVQLVISTSDYDG
                     LDKSSQTQKILLPPDRKPVVVKIPLNPERYGLHYLNVKCTSENETYVCSRNFAYLHPD
                     TRERGNWSEGKGAIFGYWNWSGSHNTPDLKTELIVMAQAGAETNLTTYRRASPEIREL
                     AEKLGYVSHAAFDGGVIYVNSFAYTNAKYYDPKNPEATQKWL"
BASE COUNT         1787 a          990 c         1153 g         1708 t
ORIGIN      
        1 ttggcagggg aggagaagat ttaagtgtgt acaattatcc tgattataat gaaaaagtgc
       61 ggaagtttgc aggggtattg ttaggcgaga tgttaccgaa gccagaagta tggactgaca
      121 atgcaattgt tgaggcaaat ttattgaaag acaaaaaaac agggagttat tatgtgtccc
      181 ttgtaaatta taatgggaaa cctgtaaaaa atttgaaggt tatgatagat gcaggacaga
      241 tagatgtcag ggaagtaaca agtgtattcc agaagtcgaa aacagaaaag aacagggata
      301 ttgttacagt gacgcttgat atcaatgatt ttgacttctt gattctaaag tagtgctttt
      361 cttgatgtgg attcaggaac atgatacagt gaaatatgac tgtgataaat tcttttcgct
      421 gcattggaag caaaacactt tttttactca cattctttcc atacttttat ttatttgcgc
      481 agaccattga atttccacgt caccctatat ttttatacac tgaccctgaa attgaagaag
      541 taaaaaagga cctaagcaaa aattctgaaa tacagacgaa aatcaagcag gcagataaaa
      601 ttattagcca accactcatt gttcctgaaa aagaaggcga ctggattttt tattattttt
      661 gtcctcagga tgacgcaatg ttgaagtatg tggaaaccgg cattcatata tgccctgtgt
      721 gtaaaaaaca atttactgat gaacgaacta atgccgctta caatacagta atcaattacg
      781 accttgacaa aaaatctgtt attgttgcca ctgcctatgc atttacgaaa gaagaaaagt
      841 atgctgcgtg ggtgaaagaa tatctgttaa cacttgcaaa gctttatcca tcttttcaga
      901 ggcacgacag atggggaaga aagggattgc ttgctgttgt tggcggaaga agatatgctc
      961 aaaatcttga tgaggcagtg tcagcaatag accttgcatc agcatatgac cttatagcaa
     1021 atgcatcctg catttccaga gacgacagac agaagatgga acaactgttg agaaatattg
     1081 tcagggaaat tctgaaatac cagatttttc atggtggcag gaacaatcac cagacctggt
     1141 ttaatgctgc gtatgcggtt gttggtcttg tgacagggga tgagacatta ttgaaagaaa
     1201 gtatttacgg aaaatacgga cttttgtggc agattgaaaa cagtattaca tctgatggtt
     1261 tatggtacga aggaacaatt gcctatcatt tttatgcttt gcaggcaatt caacacacac
     1321 ttgatgcagc cagaagggca gggcttgatt tttcaaaaaa tgaaaaactg aaatccatgt
     1381 ggcttggacc aattttgatg gcatatcctg atggtaaact tcctgttttt catgatagcg
     1441 accctgttga actgaaaaat tacagacggt tttatctatg ggcatacagg tattttaagg
     1501 atggcatctt tgcaaaatat gcgggtattg aaagcattga tggaaaagat attgaactga
     1561 aaagtacaaa tctatcaggc attggtgttg tttcactgag gaaaacattt aacgataatc
     1621 aggtgtgtgt gatgcttgat tatggaattc acggtgatag ccatggacat cctgataaat
     1681 tgaatattgt gctatttgca aatggtaaag agtttcttct tgattcagga cgaatcagtt
     1741 acagtgttcc tgaatataaa agctggtgca gaacaacaat tgcccacaat acagtcgttg
     1801 ttgacgagaa agaccagcaa ccagccacag gaacactgat ttattttgaa gacaatgacc
     1861 gatacagcgc ctgtcttgca aatgttgatt ctgcgtatcc cggatatggg ttaaaaagat
     1921 ttctgattct tgcagagaat ttacttgtgg atgttttttc tgttgaaggc gaaaaacagt
     1981 cgcagatgga ctggattatt cattgccggg gaaaggttgt tgatgatttt ttgcataaga
     2041 aagtacaaaa tctcggaaca ggaaatggtt atcagcattt aaaagatatt aagcaacttg
     2101 tttcaatctc tgaaccagtt gtgtatcaat gtgaacaaaa cgataaaatg ttaaaagttt
     2161 tcctggttaa caattctggt tgttcaatct ttactggtta cggcatcggt tatcacctta
     2221 aggacaatgt gccatttttg atgaaaaggg taaaaagaaa ttgcgcagtt tttattgctg
     2281 tgtatgattt ttcaggtagt gtgaaaacaa taaagccagt tgttgtgctg gatgaaaaaa
     2341 atcgaagaat gccagattat caggcactgg gcttgagcat tgaacttgaa aataaaacca
     2401 ttgaaatcgg gcttgattta cgagaaaaaa ttgcacagcc agtattgttt ggaaataata
     2461 agtttaagag aatttttttc aatgtgcgat aaatccgcca ccaatgatcc tgtcttcatc
     2521 atagaatact gctgcctgtc ctggtgtaat tgctctttga ggtttatcaa atactacacc
     2581 aacgcggttg tttccaattg gtgagatgat tgaatgtgct gccctgtgtt tgtatctgat
     2641 ttttgtctgt actgtcaggg gtttttcaag tgattcaatt gccataaaat taacattttc
     2701 tgcgataagt ttatccctat acaaatctat ttcttcacct accacaatga cattcttttc
     2761 tggttttatt tcaatcacat acagcggttt cttgtttgaa attccaagtc cccttctctg
     2821 tccaatagta tagtgaataa ttcctctgtg tttgcccagc accctgctat ttttatcaac
     2881 aatgtcacct ggcttcaatc cttcatcaaa aagaaaaaat ctatctccag caataaagtc
     2941 ctggctttct tcctgttcgg caatttcaat cccgcgctgc tttgcaaatt ctcttacctg
     3001 ctgttttgta taataaccta aaggaaatat cagttttgaa agttgttgtt gtgtcagaag
     3061 aaaaaggaaa taactctggt ccttgttttc gtcaatgccc ttcttcagga tgtacctgtt
     3121 gccatcaaat tccacccttg cgtaatgacc tgttgcaaaa caatcatatt caatccctga
     3181 ttgctgaact ttttccagta gaacctgaaa ttttatgaac cgattgcaca caacgcatgg
     3241 atttggcgtc tttcctgaaa gatattcttt tttgaaataa tcaagcacat atctatggaa
     3301 ttcttttttc aaatcaataa aatgtactgt tatccctgtt aatttgctga ttttctcaag
     3361 attttctatt tccttatcct ctggtccata gcacgatgat ttcatcttgc agtttacatg
     3421 attgtttttg tcccatacca tcatgaatac gccagtgaca tcatatccct gttttttcaa
     3481 caaaatcgca ctgacagttg aatcaattcc accactcatg ccaacgatta cccttttttg
     3541 cctggtcata aaaatctccc acttcattat aaattttttc aggcgcttga caaaatttta
     3601 tgaaatggtt aacttactga aaacacaaag ggggaattat gaagagcagg atctggctgg
     3661 caggtttatt tgttttgatt ttttgcacaa tgtctttagc ttctgataac aaagatattg
     3721 aaaaaattct tggctcaagt agtgaaaact ggacaattac aggtgcaaag tataacgtaa
     3781 tttcagaaaa caatattcca atccttgcca ctgccaggga cggtcatatt tccattacag
     3841 ggaaacaggt gtacaaagct cctgctgaat acaattttta tttctatctt cagccagaaa
     3901 aaccttatgg ttcaagcatc actgtttctg ctggatgcgc agatttgcct gatggttcaa
     3961 aacaggaatt tacggcttct gtcagtgctg ctgcgaatgc caattatatc aattatagtt
     4021 ttggtatcaa gccagaaact gctaaagcaa aaagtggaca tttatacttt caggcatttt
     4081 cggatatgaa tctgtcctgg ccagaagaga tgagaaagaa cattgaatgg cagattgcct
     4141 cagcgccgaa gataaatgag acattgcaca atcttcggct cgttattaca aaaacatact
     4201 tcagggttta tgttgatgga aggtttgtgg gtcagcataa agtgaaggaa ggtattgatg
     4261 gaagcggtac agtaaaactg actatgtact acggaacaaa acttgtaaat atgaaaattg
     4321 gtggaactga tgaaattgac gaaaggtttt tccctcttga attaacagga tttctcaata
     4381 gttcttcaat catcaaaggg ctcgctctcg caaaaggtga gattgaaaat ttgaaagagc
     4441 gattgaaaaa agaagaaata cctttttatg tacctgcgtc tcaaaaaggt aatgaccaca
     4501 tagaccttgg cacaagctgg acgaaatttg gagccattaa aggatacatt gcagcaaact
     4561 tcggtacctt tggcggaaga tggatttcag cggatagaat tgatagaaca cgattctgca
     4621 tgtatgttcc ttattcaaaa tacaaggcgc ttcatattat tggcaccttt gatggagaga
     4681 aattcaatgt tccagtaata acagcgcagt tttatagacc aaacgcaggt catccagcaa
     4741 attttgccgg taaggttcca ttatattcag caaaatcagg tgctgagaag atataccctg
     4801 tgaaactttc aaatggtcag acaataaatc tttttcatgt tgtaattcct gttgaccctg
     4861 gagcaatgga ttggttttca gaccttgacg tagtgggtct ggaacttaca aaggaagtaa
     4921 aaccttacag ggcatatcct gatccgcttg aatatagctg gcacggtgca ggacttccaa
     4981 gtggcgttca tatctatgct gcaacccttg aagcagtaca aacagatatg gacattcagc
     5041 cagatgctgt tggtcatgtc tggacagcac cactgaaacc atcgtacaca ataaatatcg
     5101 ttaataaaac agatacagca aaaaatgtgc agcttgtcat ttctacatca gattatgatg
     5161 gcttagataa atcaagtcag acccagaaga ttttgcttcc acctgataga aaacccgttg
     5221 ttgtaaaaat tccattaaat ccagagagat acggtcttca ttatctgaat gtgaaatgta
     5281 cttctgaaaa tgaaacctat gtttgttcaa gaaattttgc ctaccttcat cctgatacaa
     5341 gagaaagggg aaactggagt gaaggaaagg gagcgatttt tggatactgg aactggtcag
     5401 gatctcacaa cacacctgat ttgaaaacag aattgattgt gatggctcag gcaggtgcag
     5461 agacaaacct tacaacatac aggagagcaa gtcctgaaat cagagaactg gcggaaaaat
     5521 tgggatatgt atctcatgcg gcatttgacg gaggcgttat ttatgtcaat agttttgcct
     5581 atacaaatgc aaaatattat gaccccaaaa atccagaagc aacgcagaaa tggttgat
//
LOCUS       DAORYU010000080         5634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_9856_length_5634_cov_5.086754, whole genome shotgun sequence.
ACCESSION   DAORYU010000080 DAORYU010000000
VERSION     DAORYU010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5634
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9856_length_5634_cov_5.086754"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..1740
                     /locus_tag="PLW07_03660"
     CDS             64..1740
                     /locus_tag="PLW07_03660"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOL49653.1"
                     /translation="MHPPFPYIVKIKSWVCLILVFYLSAAAIPQNIADNDDPFTCAVI
                     AFNESLYEISIEFLDQYFSNPESKKNDYALFLYGINLLKLKKYGESLTKFEQFTKNFP
                     DSPYMKDAWKYTITLQMLLNRSSEAWKTYSQGIANYGRQIDIEKNLGYMLLDEVARLI
                     QSNNDASAKNILQQMEQVLPETGIVSEIQYYQALIYYKENNFEKALQKFLSALPYFKN
                     HRIEPEILLKIGDCFFNLKNYSESQKYYDQIISKFSKSLQAEWAKFHSALIYKKNMKY
                     KEAKKILTALVKATTNDEILIRSCWELGKISMLEDKKDDAVMWYNKIIVTAKDQEIIM
                     TTKLELGHLYFNQGDYSKAITLFSEYLNFKNDEDVIYALGSAFYNNNQTEQAVEIWES
                     LMKQNPGYPFSLSVLKILYNFYKEKNNRDMMKYIFNKIWEKYPEDNFILTEGIIFLNE
                     ILNTGATEEAARYLKKLDFQKNSETLFLNAKVLYLTGNYQQSEQMLKQIDKKSVFAAE
                     ALYLLIEINLAKSNVKDAQMYYVKLLASFPKSIWAQKARDSISKYNQLSK"
     gene            1758..3152
                     /locus_tag="PLW07_03665"
     CDS             1758..3152
                     /locus_tag="PLW07_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOL49654.1"
                     /translation="MFFIFFSTISALAQNIEIPETIVTGKDRNIYKTPIFIYHHDIQI
                     KFPEIPKVPQQYLKIRKVPDHKKEKIVLEHALNEFSTFGGKFGFLFSSLQFSYPEWNM
                     RLSMMNDDSFRSHDKKNVIESSFQVFPVEKFSVDFAYWNCFKEIPDTDRKKHTVLTDI
                     NFFFKDENIQWMLNGFCNTLSKVQESHGTFEIKKFVGDFTLGGAIGYDNFAGKGNGIY
                     EIAGGYANKNFQSELVLKTIGDQTKILPSMNMNFEKQNTKLQTGISSNFDFPNLWKQI
                     GQESYLDMKKIYLAPEENYAVYCEVSHKIKDVIMKVKGEITYEKISYNWKDIDNDNLY
                     EPCVLKNNTITTIDIGMAKRSKTFYIETCYTLFNREKKKSGFPENMGYIKTGFVAGKF
                     IPEIMVSYTGRKKFDTVELGGYALFSALFSYEISKNTKFFCEFNNIFNNKYREAPGYN
                     GRPFDVIAGFQTRW"
     gene            3184..3819
                     /locus_tag="PLW07_03670"
     CDS             3184..3819
                     /locus_tag="PLW07_03670"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOL49655.1"
                     /translation="MNLWQFTVRGGYLMIPIIICGALTLAVIIERLIALKRSETDAEK
                     FIGKIEEKLRKRKIREAIEICESTPGPVSQLVHTALIKSDRPPEIIRENIQDEANHII
                     PYLEKYLGVLATIATIAPLLGLLGTVTGLIKAFMVIEAKAGLVNPGDLARGIWEALIT
                     TVAGLVVSIPAYLGYNYLVSRVNGLINDMERATSRMMDILIFLHSEEEEIK"
     gene            3816..4223
                     /locus_tag="PLW07_03675"
     CDS             3816..4223
                     /locus_tag="PLW07_03675"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOL49656.1"
                     /translation="MKFPRRYEIFKGQINMAPLIDVVLLQLIYFMLTSSFIMQPGIKI
                     NLPSAMSTDKIEEKEIFINISAEGIIFLEEKQVSPEQLAGLLNSYKGKEHLVVVIKGD
                     IRTPHGKIVNVMDIVRTSGISKIAIATIPVSEQ"
     gene            4436..4933
                     /locus_tag="PLW07_03680"
     CDS             4436..4933
                     /locus_tag="PLW07_03680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOL49657.1"
                     /translation="MQKITILKEEVGRLEKNVTIGAPKTPLITIEKQENIQWELPLPK
                     TTIAGTQPPVTLNSEPQSRIYQGTITGENFEITGPGGNRAIVSKVLPEYPAWAEQQNI
                     EANIKIKIWVNKNGYVFSTEIIETTGYRKLDAIAEEALKKWKFSEIDKDVNVWAIVTI
                     KFRLQ"
     gene            4930..5208
                     /locus_tag="PLW07_03685"
     CDS             4930..5208
                     /locus_tag="PLW07_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49658.1"
                     /translation="MIQIEITLAITGYLFLSLLILFLWLFGETRKKPVLPVKKENYVW
                     QCPLCFHIYVDSSGSDISKCPLCGSLHKKHESGYNVNKENLSENQGAR"
     gene            5205..5411
                     /locus_tag="PLW07_03690"
     CDS             5205..5411
                     /locus_tag="PLW07_03690"
                     /inference="COORDINATES: protein motif:HMM:NF022229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOL49659.1"
                     /translation="MITEIGFTAGDIWNYLDKYGEVSFLKMVKAIDKPRDLALMSLGW
                     LAREGHVEVVRSGRNDFKVKLRNR"
     gene            5416..>5634
                     /locus_tag="PLW07_03695"
     CDS             5416..>5634
                     /locus_tag="PLW07_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HOL49660.1"
                     /translation="MVKVLVVYHSLGGNTKIAAEIVAEGCRRKGADVTLKTGTEATEK
                     DLIECDGIAIGSPDYFSYMAGGLKDFFDR"
BASE COUNT         2055 a          955 c         1047 g         1577 t
ORIGIN      
        1 agaaagggct ccaggccaca aacgtggtaa aactgtaagc cgaatatcac ccaggggggt
       61 gcattgcacc cccctttccc ttacatcgtg aaaataaaaa gctgggtttg tctgattctg
      121 gttttttatt tatcagccgc tgctattcct cagaatattg cagataatga tgatcctttt
      181 acctgtgctg taatcgcttt taacgaatcc ctgtatgaaa tatcaataga atttcttgac
      241 caatatttta gcaatcctga atcaaagaaa aatgattatg ccttatttct atatggaatc
      301 aatcttctaa aactgaaaaa gtatggggaa tcactaacaa agttcgaaca gtttacaaaa
      361 aactttcctg attcaccata tatgaaagat gcatggaaat atacaataac acttcaaatg
      421 ctcttaaaca ggtcttctga agcatggaaa acatattctc agggaatagc aaattatggt
      481 agacagattg acattgagaa aaatctcgga tatatgttac tggacgaagt ggcacgcctg
      541 atacaatcaa acaatgatgc ctcagcaaaa aacattctac agcagatgga acaggtactt
      601 ccggaaaccg gaatcgtttc agaaattcaa tattaccagg ctttgattta ctacaaggaa
      661 aataattttg aaaaagccct gcaaaaattt ctgtctgctc taccctactt taaaaaccac
      721 agaattgaac cagaaattct tttaaaaatt ggtgactgtt tcttcaattt gaaaaattat
      781 tctgaaagcc agaaatacta tgatcagata atatcaaaat tttcaaaatc ccttcaggca
      841 gaatgggcaa aatttcacag tgctcttatt tacaaaaaaa acatgaaata taaagaagca
      901 aaaaaaatcc ttactgctct tgtaaaagca acgacaaacg atgaaatcct gattcgttcc
      961 tgctgggaac tgggtaaaat ttctatgctg gaagataaaa aagatgacgc tgtcatgtgg
     1021 tataacaaaa tcattgtcac tgcaaaggac caggaaataa ttatgaccac aaaactggaa
     1081 ctcgggcatc tgtattttaa ccagggcgac tattcaaaag cgataacgct tttttctgag
     1141 tacctgaatt tcaaaaacga tgaggatgtc atatatgctc ttggttcagc cttctataat
     1201 aataatcaaa ctgaacaggc cgtggaaata tgggaatcct tgatgaaaca aaatcctggt
     1261 tatccgtttt cgctttcggt tcttaaaatt ctttataatt tttacaagga gaaaaacaac
     1321 agagatatga tgaaatatat ttttaacaaa atctgggaaa aatatccaga agacaatttc
     1381 attctcacag aaggtattat atttcttaat gaaatactga atacaggagc aacagaagaa
     1441 gctgccagat atttgaaaaa attggatttc caaaagaatt ctgaaacatt atttttaaat
     1501 gcgaaggttt tatacctgac aggaaattat caacagagtg aacagatgct taaacaaatt
     1561 gacaaaaaaa gcgtgtttgc tgcagaagca ttgtatctac ttattgaaat taatctggca
     1621 aaatcaaatg taaaagatgc acagatgtac tacgttaaac ttcttgcctc ttttcctaaa
     1681 agtatatggg cacaaaaggc acgggattct atatcaaaat ataaccagtt aagcaaatga
     1741 aatacctgtg tacaggcttg ttcttcattt ttttttcaac aatttcagca ttagcgcaga
     1801 atattgaaat tccagaaacc attgttactg gaaaagatag aaacatttac aaaacaccga
     1861 tttttatata tcatcatgat attcaaataa aatttccgga aatcccgaaa gttcctcaac
     1921 aatatctgaa aatcaggaaa gttcctgacc ataaaaaaga aaagattgtt ctcgaacatg
     1981 ctttaaatga gttttctaca tttggaggaa aatttggatt tttgttttca agtttgcagt
     2041 tttcctatcc agaatggaat atgcgattat ctatgatgaa cgacgattct tttagaagtc
     2101 atgataaaaa aaatgtaata gaaagttctt ttcaggtatt tcctgttgaa aaattttcag
     2161 tggattttgc ttactggaac tgtttcaaag aaatacctga taccgataga aaaaagcata
     2221 cggtgcttac tgacataaat ttctttttca aggacgaaaa cattcaatgg atgcttaacg
     2281 gattttgtaa cacactatcg aaagttcaag aatcacatgg aacttttgaa atcaaaaagt
     2341 ttgttggcga ctttacactt ggcggagcca ttggatatga taattttgct ggtaaaggaa
     2401 atgggattta tgaaattgct ggtggctatg caaacaaaaa tttccagagc gaacttgtgc
     2461 tgaaaacaat aggagaccaa acaaaaatat taccttcaat gaatatgaat tttgaaaaac
     2521 agaacacaaa acttcaaact ggtatttcaa gtaattttga cttcccgaat ttatggaaac
     2581 agataggcca ggaatcctat cttgacatga aaaaaatcta tctggcacct gaagaaaatt
     2641 atgctgtcta ctgtgaggtt tctcacaaaa taaaagatgt gataatgaaa gttaaaggag
     2701 aaattactta tgaaaaaatc tcttataact ggaaggatat tgacaatgac aatctgtatg
     2761 aaccatgtgt cttgaaaaac aatactatca ccacgattga tattggaatg gcaaaaagat
     2821 caaaaacgtt ttatattgaa acatgttata cccttttcaa tcgagaaaaa aagaaatccg
     2881 gttttccaga aaacatgggt tatatcaaaa caggttttgt tgctggcaaa ttcataccag
     2941 aaattatggt aagctataca gggagaaaaa aatttgatac agttgaactt ggcggatacg
     3001 cactgttttc agcacttttt tcttatgaaa ttagcaagaa cacgaaattt ttctgcgagt
     3061 tcaataatat attcaacaat aaatacagag aagcaccggg atataatgga agaccatttg
     3121 atgtcattgc gggttttcaa accagatggt aaatttataa tataatttat taaggagcgt
     3181 attatgaatt tgtggcaatt tacggtacgt ggcggttatt tgatgatacc aataatcatt
     3241 tgcggagcac tgactctggc tgttattatt gaacgtctca tcgcgttaaa aaggtctgaa
     3301 acagacgctg aaaaattcat aggcaaaata gaagaaaaat tgagaaaaag aaaaatcagg
     3361 gaagccattg aaatatgtga atcaacacct ggaccggttt cacaacttgt tcatacagcc
     3421 cttataaaat ctgataggcc gccagagatt ataagggaga atattcagga cgaagcaaat
     3481 cacatcattc cgtatcttga aaaatacctc ggggttctcg ccacgattgc aacaatagcg
     3541 ccacttctcg gattgctcgg aactgtgaca ggtttgataa aagcatttat ggtaattgaa
     3601 gccaaagcag gactggtaaa tccaggagac ctcgcaagag gtatatggga agcactgatc
     3661 acaactgttg caggtcttgt tgtaagcata ccggcttatc ttgggtataa ctatcttgtt
     3721 tctcgtgtaa acggattgat caatgatatg gaacgtgcca catcaagaat gatggatatt
     3781 ttgatttttt tacattctga agaagaggaa ataaagtgaa attcccaaga cgctatgaaa
     3841 tcttcaaggg tcagattaat atggcaccat tgattgatgt tgtgctactg caactgattt
     3901 attttatgtt gacttccagt ttcatcatgc aaccaggcat aaaaataaac ctgccttcag
     3961 ccatgtcaac agataaaatt gaggaaaaag aaatttttat caatatttca gcagagggta
     4021 tcatttttct ggaagaaaaa caggtctcgc cagaacaact tgcaggttta ctgaattctt
     4081 ataaagggaa agagcatctt gttgttgtga tcaagggaga tataagaact cctcatggaa
     4141 aaattgtcaa tgtgatggac attgtaagaa catccggcat atcaaaaatt gccatagcca
     4201 caatacctgt ttcagaacaa tgattcaaac agatgagaaa aatcttttaa gaattttttt
     4261 attgtgcctt gccgcacaca ttatgatact gccaatcttc agttttgtac tcccgccaaa
     4321 aagcaagaaa ccgaagtatc aaatagtttt tctttatcag gaaccacact caacaacaac
     4381 aaatatacca aaagtgagaa ttaatccaca attacctgtt accaggaaaa acccagtgca
     4441 aaaaataact atactgaaag aagaagttgg acgccttgaa aaaaatgtca caataggcgc
     4501 gccaaaaaca cctctaatca ccattgaaaa acaggaaaat atccagtggg aactcccact
     4561 tccaaaaacc acaatagcag gaactcagcc accagttact ctgaattcag aaccacaatc
     4621 gcgaatatat cagggaacaa ttacgggaga aaactttgaa ataacaggtc ctggcggaaa
     4681 cagagccatt gtttcgaaag tgttaccaga atatcctgca tgggcagaac aacagaatat
     4741 agaagcaaac ataaaaataa aaatatgggt aaacaaaaat ggttatgttt tttcaactga
     4801 aataattgaa acaacaggat acagaaaact cgacgccatt gctgaagaag cattaaaaaa
     4861 atggaagttt tcagaaatcg ataaagatgt taatgtctgg gcaatagtga caatcaaatt
     4921 ccgattacaa tgattcaaat agaaataact cttgcaatca caggatatct atttttaagc
     4981 cttttgattc ttttcctgtg gctattcggt gaaacaagaa aaaaaccggt tttgccagtc
     5041 aaaaaagaaa attatgtatg gcaatgccct ctgtgctttc acatctacgt ggacagttct
     5101 ggttctgaca tttcaaagtg cccattatgc ggaagtcttc acaaaaaaca cgaatcaggg
     5161 tataatgtta ataaggaaaa tctttcagaa aaccaggggg caagatgata acagaaattg
     5221 gattcactgc cggagatata tggaattatc ttgacaaata tggagaagtt tcatttttaa
     5281 agatggtcaa agccattgat aaaccaaggg atcttgctct tatgagtttg gggtggctcg
     5341 ccagagaagg acatgttgaa gtcgtgagat ctggaaggaa cgattttaaa gtgaaactac
     5401 ggaatcgtta acgaaatggt caaggttctt gttgtgtacc actcgctcgg cggaaacaca
     5461 aaaatcgcag cagaaatagt ggcagaagga tgccgtagaa aaggggccga tgtaactttg
     5521 aaaaccggca ctgaagccac agaaaaagac cttattgaat gtgacggcat tgccatagga
     5581 agccctgatt atttcagcta tatggcaggt ggattgaagg atttttttga taga
//
LOCUS       DAORYU010000081         5591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_9948_length_5591_cov_4.162211, whole genome shotgun sequence.
ACCESSION   DAORYU010000081 DAORYU010000000
VERSION     DAORYU010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5591
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9948_length_5591_cov_4.162211"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3020
                     /locus_tag="PLW07_03700"
     CDS             <1..3020
                     /locus_tag="PLW07_03700"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOL49661.1"
                     /translation="RVSQGSTGAIVCLDKRKSVFTSEVIKFLENSAKAILVTLDRCES
                     TKLLQDTIANQEAFLNATDDMTFVKDVNFRYINLNKAYARFFKKPVEEILGKTDFELM
                     EYSAAKRCRETDHQALKEGKLVINEEIVGERVYETRKFPVKLQSGETGVGAFIRDITE
                     MKQSKQKIEKLMWMYSMLYQVNQAIVRAKNTQQLFSDACEIACKEGNFVLAWIGIADY
                     EKNIVAPVAGSRQKREYYKDIKISLDPLVPEGKGPTARSIRTGKICICNNILEDEKMK
                     SWWQRAKKYGFCSSAAVPLRVHGKVIGALNFYSDQPYFFNRDIEKLLFEVSTDIGFCI
                     EKLNAEDIRKKAEKKLRENELHLRSIFDYSPYPVAELDCSQIKNAFKKINPQDLEKYF
                     VENPATIDTLKKSIMILNSNKQMFEFFNTTQIEKLSEFLSKVVVEPEIIRCLISEKKC
                     EQFQTKIDCDENIKKEVLINVTLLPGHEKDWSRLLVSMIDITERIYMEQNLRATLSRF
                     QGFFDTAATGMGILDLEGRPIALNKRICEMLGYEHQELMKMKLTDVVPPEDRETVEKI
                     YEKLAKGESIHYETVERKYVRKDGSIMYAYVSAGLIYDHILKKHCVTAVVVDITKQTQ
                     YLQQIERIQNLLKAYAECNEIIAKAKEEQEMLVSICNELTGANLGFLFIVLRVDSNFD
                     VVACSEGNTDFLSELKNLFIAERKACPTVDSLFENKFLVINDIMSADYSDKWKQLVLQ
                     HGFHSAAVFPIVFEGNSIGSLTIYSSDKNASREENEISILRAIVEDVGYGITMIRARK
                     EKESATMGLQQSYERLQKTIEGISLAIAKIVEARDPYTAGHQKRVAQLSLAIAKEMGL
                     AENIVQGIGISAILHDIGKISVPVEILVKPGKLAEDEFNIIKLHSGIGYEILRQIPFP
                     WDIARIVLQHHERLNGSGYPDGIMENDILQEAKIIAVADVVEAMASNRPYRPALGIDV
                     ALNEIKNKSGILFDSEVVDICIRLFKEKGFSFD"
     gene            3035..3247
                     /locus_tag="PLW07_03705"
     CDS             3035..3247
                     /locus_tag="PLW07_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49662.1"
                     /translation="MFFRKKTKFKPGDIVYHRNTFEKGKILRQDALDQKKWVVEWGTS
                     VKTHLEEELMSHDEFVSSEIDKKTKI"
     gene            3268..3426
                     /locus_tag="PLW07_03710"
     CDS             3268..3426
                     /locus_tag="PLW07_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007502288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49663.1"
                     /translation="MSKLVRFLYQLARTANDIEKFSSGDPKKITRRIKNKIIGRKLGS
                     KIYRWPKI"
     gene            complement(3431..3907)
                     /locus_tag="PLW07_03715"
     CDS             complement(3431..3907)
                     /locus_tag="PLW07_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HOL49664.1"
                     /translation="MLYKKFLKLAASIVLCLLAGAIGSIFTSSSVGNWYRTLNKPIFN
                     PPDWIFAPVWTLLYIMMGISLFMVWTAEMEKSLKRMAFLIFAIQLILNILWSVLFFGL
                     QNPVLSLIEIFVLWIAILIAILVFKKISILSAFLLMPYLAWVTFAAFLNYSIVTLN"
     gene            complement(3913..4920)
                     /locus_tag="PLW07_03720"
     CDS             complement(3913..4920)
                     /locus_tag="PLW07_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HOL49665.1"
                     /translation="MAKQQKGNPVRITKQGDWVTYRPDIKVLDCTIRDGGLMNDHKFD
                     DEFVKAVYTACVEAGIDYMELGYKSSKKIFAPTEFGPWKFCDEDDVRKIVGDNNTDLK
                     LSVMADAERTDYHQDILPAKDSVFDLVRVATYIHQIPAALDMIKDAHDKGYETTLNLM
                     AISTVQDRELNEALEVIAKSEVRVIYLVDSFGALYSEQIRDLTKKYLKYAKATGKEVG
                     IHTHNNQQLAYANTIEALILGASWLDASMAGLGRGAGNCQIELLLGFLHNPKFHLRPV
                     LQCIRDWIEPLRSQLRWGFDIPYMLTGQLNQHPRAAMKFMSGENWKDIVEFFDSLAEK
                     E"
     gene            complement(4972..5265)
                     /locus_tag="PLW07_03725"
     CDS             complement(4972..5265)
                     /locus_tag="PLW07_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49666.1"
                     /translation="MEGGFYKGENERRNFEKREIEKIFVKREDKRRNFAKGKRKLRVY
                     MEVNKRWRFIKGIKKNAVLKRKNYRKKFVNSSNGIRKNMELILYFCIHDEFIV"
BASE COUNT         1821 a         1007 c         1066 g         1697 t
ORIGIN      
        1 taagggtttc acaaggttct acaggtgcca ttgtttgtct ggacaaaaga aaatctgtgt
       61 ttacttctga agtgataaaa ttccttgaga attctgcaaa ggcgatttta gtaacccttg
      121 acagatgcga atcaactaaa ttactgcagg ataccatcgc aaatcaggag gcgtttctca
      181 atgcaacaga cgatatgact tttgttaaag atgtaaattt cagatatatc aacttgaaca
      241 aagcatatgc gcgatttttc aaaaaaccag tcgaagaaat tcttggtaaa acagattttg
      301 aactgatgga atacagtgca gcaaaacgat gtcgcgaaac agaccaccag gcattgaaag
      361 aagggaaact ggtcattaat gaagaaattg ttggtgaaag ggtttatgaa acaagaaaat
      421 tccctgtaaa actgcagtca ggagaaacag gtgtgggcgc ttttatcagg gatattactg
      481 aaatgaagca gtcgaaacag aaaattgaaa aacttatgtg gatgtattca atgctttatc
      541 aggttaatca ggcaattgta agggcaaaaa atacgcaaca attgttttcc gatgcctgtg
      601 aaattgcctg caaagaagga aattttgttc ttgcctggat aggtattgcg gactacgaaa
      661 agaacattgt tgcgcctgtt gctggttcaa gacagaaaag agagtattat aaagatatta
      721 aaatttctct tgacccactt gttccagaag gtaagggacc tactgcacgt tcaattagaa
      781 caggcaaaat ttgtatctgt aataatatcc ttgaagatga aaaaatgaaa tcatggtggc
      841 aaagggcaaa aaaatatgga ttttgttctt ctgctgctgt gccattaaga gtacatggga
      901 aagtaattgg tgcattgaat ttttattcag accagccata tttctttaac agagatattg
      961 aaaaactttt gtttgaggtt tctactgata tcggattttg tattgaaaaa ttgaatgccg
     1021 aagatataag aaagaaagca gaaaaaaaac tgcgtgaaaa cgaattgcac cttcgttcaa
     1081 tttttgatta ctcgccgtat cctgtggcag aactggattg ttcgcagata aaaaacgcat
     1141 ttaaaaagat taatccgcag gaccttgaaa aatattttgt tgagaatccc gcaactattg
     1201 atacattgaa aaaatccatc atgattctaa acagcaacaa acagatgttt gaatttttta
     1261 ataccacaca aattgaaaaa ctgtctgaat ttctgtcaaa agtcgtcgtt gaaccagaaa
     1321 ttatcagatg cctgatatct gaaaaaaagt gcgagcaatt tcagacaaag attgattgtg
     1381 atgaaaacat caaaaaagaa gttcttatca atgttacttt gctgccagga catgaaaaag
     1441 attggtcaag gttacttgtt tcaatgattg atattactga acgcatttat atggaacaaa
     1501 accttagagc aacactcagc agatttcagg gattttttga tacagcagca actggtatgg
     1561 gtattctcga tcttgaaggc agacccattg cattgaacaa acgcatatgt gaaatgctcg
     1621 gatacgagca tcaagaactg atgaaaatga aacttacaga tgtcgtgcca cctgaagata
     1681 gagaaactgt agaaaaaatt tatgaaaaac tggcaaaagg tgaaagcata cactatgaaa
     1741 cagtggaaag aaagtatgta agaaaagatg gttcaattat gtatgcctat gtttctgcag
     1801 gattgattta tgaccatatc ttgaaaaaac attgtgttac agcagttgta gttgacataa
     1861 caaagcaaac acaatacctg caacagattg aaagaattca aaatcttttg aaggcgtatg
     1921 cagagtgtaa tgaaattatt gcaaaagcaa aagaagaaca agagatgctt gtttcaatct
     1981 gcaatgaact aacaggagca aatttaggtt ttcttttcat tgttttaagg gtggattcta
     2041 attttgatgt tgttgcgtgt tcagaaggta atacggattt tctatcagaa cttaaaaatt
     2101 tatttattgc agaacgaaaa gcatgtccaa cagttgattc attgtttgaa aacaaatttc
     2161 ttgttatcaa cgacataatg agcgctgatt attcagataa atggaaacaa cttgtacttc
     2221 agcacggttt tcattcggct gcagtatttc ctattgtttt tgaaggcaat agcatcggta
     2281 gtttaaccat ctattcatct gacaaaaacg catccagaga ggaaaatgag atttctattc
     2341 tccgtgctat tgttgaagat gtaggatacg gaataacaat gatacgagca agaaaagaaa
     2401 aagagtcagc aacaatgggt cttcaacaaa gttatgagcg acttcaaaaa acaatagaag
     2461 gtatttcact ggcgatagca aaaatcgttg aagcaagaga cccttacaca gcaggtcacc
     2521 agaaacgtgt tgcgcaatta tctttggcga tagcaaaaga aatgggactg gcagaaaaca
     2581 ttgttcaggg aataggaatt tctgcaatcc ttcacgatat cgggaaaatt agtgttcctg
     2641 tagaaattct tgttaagcca gggaaacttg cagaagatga atttaatatt attaaacttc
     2701 attctggcat aggttatgaa attttgaggc agattccttt tccatgggat attgcgagaa
     2761 ttgtgttaca gcaccatgaa agattgaatg ggtctggtta tccagatgga atcatggaaa
     2821 atgacatttt gcaggaagca aaaattattg ctgttgctga tgtggttgaa gcaatggctt
     2881 ctaacagacc ttacagaccc gctttaggta ttgatgtagc ccttaatgaa atcaaaaata
     2941 aaagtggcat tctgtttgac tctgaagttg ttgatatctg tatcaggtta ttcaaggaaa
     3001 aaggtttttc ttttgactga agggaggtgg atttatgttt tttagaaaaa aaacaaagtt
     3061 caagccaggt gatattgttt atcatcgcaa tacttttgaa aaaggaaaaa ttttaaggca
     3121 ggacgctctg gaccagaaaa aatgggtggt tgagtggggt acgagtgtga aaacgcatct
     3181 ggaagaagaa ttgatgtccc atgacgaatt tgtatcttct gaaattgata aaaaaacgaa
     3241 aatttagttt tttcacgggg gctggtaatg agcaaacttg tcaggtttct atatcaactt
     3301 gcaaggacag caaacgatat tgaaaagttt tcaagcggag acccgaagaa gataacaaga
     3361 agaataaaga ataagattat cggcagaaaa ttgggaagca agatatatcg ttggcccaaa
     3421 atatgatgtg ttaatttaac gtcactattg aataattcaa aaaagccgca aaggtaaccc
     3481 atgcaagata cggcataagc aaaaaagcag aaagtatgga gatttttttg aataccaaaa
     3541 tagcaatgag tatagcaatc cacaaaacga atatttcaat aagagataaa acagggttct
     3601 gcagtccaaa aaataagaca gaccataata tgttcaatat caattgaatg gcaaaaatca
     3661 aaaaagccat tctttttaat gatttttcca tttcagcagt ccacaccata aagagtgaaa
     3721 tgcccatcat tatgtagaga agtgtccaga caggagcaaa tatccagtca ggcggattaa
     3781 aaataggttt attcagggtt ctgtaccagt ttccaacaga acttgaagta aagatagatc
     3841 cgatggcacc tgccagcaaa cagagaacaa ttgaagccgc aagtttcaga aattttttgt
     3901 atagcattat ttctattctt tttctgccag agaatcaaaa aactccacaa tgtctttcca
     3961 gttttctcct gacataaatt tcattgctgc gcgtggatgc tggtttaact ggcctgtgag
     4021 catataaggg atatcaaatc cccagcgtag ttgagacctc aatggttcaa tccagtccct
     4081 gatgcattgc agtactggcc gcaggtgaaa tttcggattg tgcaggaacc caagaagtaa
     4141 ttcaatctgg cagttccctg caccccgacc aaggcctgcc atacttgcat caagccagct
     4201 tgcgccaaga ataagcgctt caatggtatt ggcgtaagca agttgctggt tgttatgtgt
     4261 gtgtataccc acttcctttc ctgttgcctt tgcgtatttt aagtattttt ttgtcagatc
     4321 tctgatctgt tcactgtata gagcgccgaa actatcaaca agataaatga cccttacctc
     4381 tgattttgca atgacttcca gtgcttcgtt caattctcta tcctggactg tggaaattgc
     4441 catcaaattt agtgttgttt catatccttt atcgtgggca tcttttatca tatcaagtgc
     4501 tgctggtatc tgatggatat aagttgcgac acgcaccagg tcaaatacac tatcttttgc
     4561 aggtagaata tcctggtgat aatctgttct ttctgcatca gccattacag acagtttcaa
     4621 atctgtatta ttgtcaccga caatttttct cacatcatcc tcatcacaga atttccatgg
     4681 accgaattca gtgggcgcaa aaattttttt tgatgatttg tagccaagtt ccatatagtc
     4741 aatgccagct tccacgcatg ctgtgtacac tgcttttaca aattcatcgt caaacttgtg
     4801 atcattcatc aatccgccat cacgaatagt gcaatcaaga accttgatat caggtctgta
     4861 tgtaacccag tcaccctgtt ttgttatcct tacaggattt cctttctgtt gttttgccat
     4921 atttgctcct ctattggttg cgaaattgcg aagaaattat accacacatt tttaaacaat
     4981 aaattcatca tggatgcaga agtataaaat taactccatg ttttttctta tcccgttgct
     5041 gctatttaca aactttttcc tgtaattttt cctcttcaaa actgcatttt tctttatccc
     5101 ctttataaaa cgccacctct tattcacttc catataaacc ctaagctttc tttttccctt
     5161 tgcaaaattc cttcttttat cttccctctt tacaaatatt ttttctattt ccctcttttc
     5221 aaaattcctc ctttcgtttt cccctttata aaatcctcct tccatttccc cccttttcaa
     5281 attcctcctc ccattctccc cctttacaaa gtccttcttt tgtttccccc ctttataatt
     5341 cctctctttt ttcccccttt ataaaagggg gattaagggg gatttcagaa atccatcccg
     5401 gccttcctct accctctcgt tttccccctt tacaaattcc tcctccaggt ttcccccttt
     5461 ataaaagggg gattaagggg gatttaaaat tgtactgccc cccatttgtc aagagcaaaa
     5521 taagggagaa ttcagagtcc tggttaaagg taattgtttt atctcttttt gttcaaattc
     5581 cactggcgtc a
//
LOCUS       DAORYU010000082         5540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_10043_length_5540_cov_4.326345, whole genome shotgun sequence.
ACCESSION   DAORYU010000082 DAORYU010000000
VERSION     DAORYU010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5540
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10043_length_5540_cov_4.326345"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PLW07_03730"
     CDS             complement(<1..322)
                     /locus_tag="PLW07_03730"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOL49667.1"
                     /translation="MVLSIVIPVFNEGQTIRDVVFQVKNADTDSFEKQIIIVDDGSTD
                     NTPYILQEIQKNCSEIVILRHTHNRGKAAALKTGFQAATGDIIIIQDADLEYDPSDYN
                     KLIEP"
     gene            complement(347..619)
                     /locus_tag="PLW07_03735"
     CDS             complement(347..619)
                     /locus_tag="PLW07_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49668.1"
                     /translation="MITWVILILIMGLMLLFFPEKIVENDWVKQIGLAITLICIGIGV
                     RMTALRRKGKREKLETKIKELEKEIETLRSQISQAQAAEEKKEENQ"
     gene            complement(632..1870)
                     /locus_tag="PLW07_03740"
     CDS             complement(632..1870)
                     /locus_tag="PLW07_03740"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOL49669.1"
                     /translation="MRTIKVGVIGVGRGMGFALGAEHVGMKLVAICDKWEEKLKEAEK
                     KLKVKTYTDYDSFLSHDMDAVVLANYFHQHAPFAIKALKAGKHVLSETSCNSTLAEGV
                     ELCRTVEKTNRIYMLAENYPFTVFNMEMRKLYQSGEIGEVTYAEGEYNHPMSPETICS
                     ISPGINHWRNWLPPTYYCTHALAPLVCITEKMPVKVNGLSIARKQINKHTARRSDPGF
                     VILCRMDNGAVFRLFGLVLPGHSNWYRIHGETGAMEITKGPGYFGPGLIRVWHEPWTR
                     KKNQVLEKTYVPEWPEKGELANRAGHGGGDFWVNWYFAQAIRTGKQPFLDVYRGVAMS
                     SVGILAWKSALEDGKPYEVPDFKKESERKKYENDDWSPFPGAKNPIPPSILGFIEPTP
                     DDLVFARKIWKKIGYTGDDH"
     gene            complement(1892..2998)
                     /locus_tag="PLW07_03745"
     CDS             complement(1892..2998)
                     /locus_tag="PLW07_03745"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOL49670.1"
                     /translation="MGKINVGIAGSCGRGRSFKIGIEASGVLKIHAVCDIDKEKLEES
                     KIALGAQEKYLDYDEMLEKSDIQAVIIGTPMNFHAPQAIAALNKGIHVLSEVPAAVSI
                     QECKELVHAARKSKAVYMMAENYTYLKTNVIVREIVKRGLFGTTYYAEGEYIHELKQL
                     NEITKWRRKWQTGINGITYGTHSLGPVLQWMSSDRVKSVCCAGSGHHYKDPRGNFYEN
                     EDSCVMLCKMKSGGLVKIRVDMLSDRPHAMTNYQLQGTDGCYESARASGERNRIWLRS
                     KCRDMTWIDIEQLADEFLPEEWKKQEEIAMKTGHGGGDYFEIIDFVNAITGERKCPIG
                     IDEAMDMTLPGLVSQQSIAQNGMWLEVPDSRKWK"
     gene            complement(2985..3263)
                     /locus_tag="PLW07_03750"
     CDS             complement(2985..3263)
                     /locus_tag="PLW07_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49671.1"
                     /translation="MKDWEWLFCAFSTVLPEQAKSTGDSRNGVWQVVDYQTDDFSGTL
                     ILAQPTSNAPDVTVKNGLFAGFLLPFRAQKLINTIMDYQCKRDNYGKN"
     gene            complement(3386..4516)
                     /locus_tag="PLW07_03755"
     CDS             complement(3386..4516)
                     /locus_tag="PLW07_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HOL49672.1"
                     /translation="MKKRLFTPGPISVPEDVLLEMAKPIIHHRTPEFLEIAKQVIEDL
                     KYFFQTKNDVFIIAGSGTAAMEAAVVNVLSPGEKALVISGGKFGERFVELVKTYGAEA
                     IIVEPGWGNPVELKNIETILKQNPDIKAVFATLVETSTGTSYDIEGLGKLISNFPETM
                     FIVDGISGLGAVECRTDLWNIDILVSGSQKALSLPPGLSFISVSEKAWKTINNCTTPK
                     YYLDLKKYKKAMEKFDFPFTMAVSLVCGLKKSLEKIKQQGLENLWEMHKKRAIAVRNA
                     AIAMGLEVFSKSPSDAVTAILLPQSIDGEKFCSDMRKEFGMSVAGGQDKLKGKVIRIS
                     HFGWQDEFDTIAVIAGIEIMLKKYKHNVELGAGVREAMKVLF"
     gene            complement(4516..>5540)
                     /locus_tag="PLW07_03760"
     CDS             complement(4516..>5540)
                     /locus_tag="PLW07_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HOL49673.1"
                     /translation="DHYLEVEFDLSKVLFITTANTEFTIPPPLLDRMEVIHLPGYTSW
                     EKLQIAKHFLIPRLLPQHGLKNEEIQISDNAINKIIKEYTREAGVRNLERQLAGVCRK
                     VARKIVETKNIKLPCRVGVNNLAEYLGPEKYTSLTKAKKPAVGVATGMAWTEYGGDIL
                     FTEVLLMKGKGKLIITGQLGSVMKESAQAALSFVRANAETLGISPDFAEKYDIHIHVP
                     EGAIPKDGPSAGVTIITALVSALTGKKARCDVAMTGEITLQGKVLKIGGLKSKILAAH
                     RAEISTIIIPKENEKDLEEIPEIVRKELKIVFVDNLMDVLNIALIESEKANKNEIFPI
                     QQGAKI"
BASE COUNT         1435 a         1246 c          941 g         1918 t
ORIGIN      
        1 ctggttcaat gagtttatta taatcagaag ggtcgtactc taaatcagcg tcctgaataa
       61 tgatgatgtc tccagttgct gcctgaaagc cagtttttaa ggcagcggct tttcctctgt
      121 tgtgagtgtg ccgcaatatt acgatttcac tgcagttttt ttggatttct tgcagaatat
      181 acggggtatt gtcagtagaa ccatcatcaa caataataat ttgtttttca aaactatcgg
      241 tgtctgcgtt ttttacctga aaaacaacat ccctgattgt ttgtccctca ttaaaaacag
      301 ggattacaat ggataaaacc atgttgattg aataagaaca caattcttac tggttttctt
      361 cttttttttc ttcggctgcc tgcgcctgag aaatttgact tctcaaggtt tctatttctt
      421 tttccagttc cttaattttt gtttcaagtt tttctctttt tccttttctt ctcagtgctg
      481 tcatccttac gcctatacca atacatatca atgtgattgc aagaccgatt tgtttaaccc
      541 agtcgttctc aacaattttt tcgggaaaga aaagcaacat caatcccata atcaatatta
      601 agattaccca cgtaatcata gtgcctcctt gttaatgatc gtctcctgta taaccaattt
      661 ttttccagat ttttcttgca aaaacaagat catcaggggt tggttcaatg aaaccaagga
      721 tgcttggtgg tattggattt tttgctccag gaaatggaga ccagtcatca ttttcgtact
      781 ttttcctttc actttctttt ttgaagtcag ggacctcgta tggtttccca tcttcaagcg
      841 cacttttcca tgcaagaatg ccaacagaac tcattgccac gccccgataa acatccagaa
      901 atggttgttt ccctgttctt attgcctgag cgaaatacca gtttacccag aaatctccac
      961 caccatggcc tgcacgattt gctaattcgc ctttttctgg ccactcagga acatatgttt
     1021 tttcaagaac ctgattcttt tttcttgtcc atggttcgtg ccagaccctg ataagtccag
     1081 gaccaaaata tcctggacct tttgtaattt ccattgcacc tgtttctcca tgaatcctgt
     1141 accagttgct gtgacctggt agtaccagtc caaaaagtcg aaaaactgct ccattgtcca
     1201 tcctgcacaa tattacaaaa ccagggtctg atcttctggc agtatgttta tttatctgtt
     1261 ttcttgcgat tgaaagtcca ttgactttaa ccggcatttt ttctgtaatg cacacaagtg
     1321 gtgcaagggc atgcgtgcaa taatatgttg ggggcagcca gtttcgccag tggttgatac
     1381 caggagagat tgaacatatt gtttctggag acattggatg attatattcc ccttcagcat
     1441 aggtcacttc ccctatttcg ccactttgat acaattttct catttccata ttgaaaacag
     1501 tgaaaggata attttctgca agcatataaa tacgattggt tttctcaacg gttctgcaca
     1561 gttcaacacc ttctgcaagc gtgctgttgc atgaggtttc actcaggacg tgtttgcctg
     1621 ctttcaatgc cttgattgca aaaggcgcat gctgatggaa gtaatttgca agtacaacag
     1681 catccatatc gtgagagagg aaactgtcat aatctgtata tgtctttacc ttaagttttt
     1741 tctctgcttc tttcaacttt tcttcccact tatcacatat tgctacaagt ttcatcccga
     1801 catgttctgc accaagagca aaacccattc ctctgcccac accgatgacc ccgaccttta
     1861 ttgttctcat aatacctcca tcaagaacat tttatttcca tttccgtgaa tcaggaactt
     1921 caagccacat tccattttgt gctattgatt gctggctgac aagtcctgga agcgtcatat
     1981 ccatcgcttc atctatgcca atgggacact tcctttctcc tgtaattgca ttgacaaagt
     2041 caatgatttc aaagtaatct ccaccaccat gcccggtttt cattgctatt tcttcctgtt
     2101 ttttccattc ttcgggaaga aattcgtctg caagttgttc aatatcaatc caggtcatat
     2161 ccctgcattt tgaacgcagc catattctgt ttctttctcc tgatgccctt gcagattcat
     2221 agcagccatc tgtaccctgt aactggtagt ttgtcattgc atggggtcta tcagaaagca
     2281 tatccaccct gatttttaca agtcctccac ttttcatttt gcatagcata acacaggaat
     2341 cttcattttc ataaaaatta cctcgcgggt ctttgtaatg atgaccactt ccagcacagc
     2401 agacagattt taccctgtca gaagacatcc actgcaatac tggacctaaa ctgtgggtac
     2461 catatgtgat tccattgatg cctgtttgcc actttcttct ccattttgta atttcattga
     2521 gttgtttgag ttcatggatg tattcacctt ctgcataata ggttgttcca aacagtcccc
     2581 tttttacgat ttcccttaca atcacatttg tttttaagta ggtatagttt tctgccatca
     2641 tatatactgc ttttgatttc cttgccgcat ggacaagttc tttacattcc tgtattgaga
     2701 cagcagcagg gacttcactg agaacatgaa tccctttatt taacgcagcg attgcctgtg
     2761 gtgcatggaa attcataggc gttccaataa tgactgcctg aatatctgat ttttcaagca
     2821 tttcatcata gtcaagatat ttttcttgtg ctccaagagc aatttttgat tcttcaagtt
     2881 tttctttatc tatatcacat acagcatgga ttttcaatac tccgcttgct tcaatcccaa
     2941 ttttgaaaga acgacctctt ccacaagaac ctgcaatacc aacattaatt tttcccatag
     3001 ttatctcttt tacattgata atccataatt gtgtttatta atttttgtgc cctgaagggt
     3061 aacaagaatc ctgcaaaaag gccatttttc actgtcacat caggcgcatt gcttgttggt
     3121 tgagcaagaa tcagtgttcc tgaaaaatca tctgtctggt aatcaacaac ctgccatact
     3181 ccatttcttg aatcacctgt gctttttgcc tgttctggta gtactgttga aaaagcacaa
     3241 aacaaccatt cccaatcttt catgagcact cctggtgaga aaggacaacg ggtaaacatg
     3301 aggaagcaga gatatataca acatatctac tgtatattgc gtcaattttc ggttgcgtat
     3361 cagaatccaa cattttttgc agggattaaa aaagtacctt cattgcttcc ctgacacctg
     3421 cacctaattc cacattatgt ttgtattttt tgagcattat ttcaatgcct gcaatcacag
     3481 caattgtatc aaattcatcc tgccagccga agtgtgaaat tctgatgact ttccctttga
     3541 gtttatcctg accaccagca acagacatac caaattcttt tctcatgtca gagcaaaatt
     3601 tttcaccatc aatgctttgt ggcaaaagaa ttgctgtaac tgcatcagat ggcgattttg
     3661 agaatacttc cagtcccatc gcaattgcgg catttcttac tgctatcgcc cttttcttat
     3721 gcatttccca taaattttca agaccctgtt gtttgatctt ttcgagtgac tttttcaatc
     3781 cgcaaacaag acttacagcc attgtgaatg gaaaatcaaa tttttccatt gcctttttat
     3841 acttttttaa gtccaggtaa tattttggag tggtacagtt attaattgtt ttccatgctt
     3901 tttcactgac agaaataaac gaaagtcctg gtggtaaact taatgctttc tgagaaccag
     3961 acacaagtat atctatattc cacaaatctg ttctgcattc aacggcccca agtccactaa
     4021 taccgtcaac aatgaacatt gtttcaggaa aattagaaat cagttttccc agaccttcaa
     4081 tatcgtaaga agttcctgtg gacgtttcta ccagggttgc gaaaaccgct tttatgtcag
     4141 ggttttgttt aagaatggtc tcaatatttt ttaactcaac gggatttccc catcctggtt
     4201 caacaataat ggcttcggct ccgtatgttt tcaccagttc cacgaatctt tctccgaatt
     4261 taccgccaga aatcacaagt gccttttctc ccggtgaaag aacattcaca accgctgctt
     4321 ccatcgcagc agttccagaa ccagcgatga taaatacatc attttttgtc tgaaaaaagt
     4381 atttcaggtc ttcaattacc tgtttcgcta tttcaaggaa ttctggtgtt ctgtgatgaa
     4441 tgatgggttt tgccatttca agaagaacat cctcaggtac agatatgggt cctggagtaa
     4501 aaagtctttt tttcatcata tctttgctcc ttgctgaatg ggaaaaattt cattcttgtt
     4561 tgctttttct gattcaatca atgcaatgtt gagaacatcc atgaggttat caacaaaaac
     4621 aattttcaat tcttttctta ctatttccgg gatttcttca aggtcctttt cgttttcttt
     4681 cggtatgatg attgttgaaa tctctgccct gtgagcagca aggattttgc ttttaagtcc
     4741 accgattttt aataccttgc cctgcaacgt gatttctcct gtcattgcaa catcgcacct
     4801 tgctttttta cctgttaacg ctgaaacaag tgcagtaata attgtcacgc ctgctgaagg
     4861 tccatctttt ggaatagctc cttctggaac atgaatgtga atatcatact tctctgcaaa
     4921 atccggagat attccaagtg tttcagcgtt agccctgaca aaactcaatg ctgcttgtgc
     4981 agattctttc atcactgacc caagttgtcc tgtgataata agttttcctt ttcctttcat
     5041 cagcaacact tcggtaaaaa gtatatctcc accatattct gtccatgcca tccctgtcgc
     5101 aactcccaca gcaggttttt ttgccttcgt cagggatgta tatttttctg gtccaagata
     5161 ttcagcaaga ttattcactc caaccctgca gggaagtttg atgttttttg tttcaacaat
     5221 ttttctcgcc acctttctac atacaccggc aagctgcctt tcaagatttc tcactcctgc
     5281 ttctcttgtg tattctttga taattttgtt tattgcatta tctgagattt gtatctcttc
     5341 atttttcaat ccatgctggg gtaaaagtcg tggaataaga aagtgttttg cgatttgaag
     5401 tttctcccag ctggtataac ccggtagatg tatcacctcc atcctgtcca gcagtggcgg
     5461 agggattgta aattctgtat ttgccgttgt gataaaaagc acctttgaaa ggtcaaactc
     5521 cacctcaaga taatggtctg
//
LOCUS       DAORYU010000083         5497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_10125_length_5497_cov_4.746784, whole genome shotgun sequence.
ACCESSION   DAORYU010000083 DAORYU010000000
VERSION     DAORYU010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5497
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10125_length_5497_cov_4.746784"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="PLW07_03765"
     CDS             <1..806
                     /locus_tag="PLW07_03765"
                     /inference="COORDINATES: protein motif:HMM:NF012524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOL49674.1"
                     /translation="IAEVWEKSVMVLWEKGAQIKTEYDQPDDPFSRDATMVMVVNEPF
                     SEPRIHLGFPGGMEDLEKYRQEVVLGVHDHWIKPEEGKWTYTYHQRLFNYPVSEGNTV
                     NQVEYIIDKLSRVFYTRRAQAITWVPSYDPETDDPPCLQRIWCRIVTEKDNHYLRMNT
                     HWRSRDAYRAAYMNLFGLTELQKYIADEISKRTGKEILVGPYVDITDAYHIYGSNFCD
                     FKDRFLKMMDTRDFYNQDRLKSRTMRSDDPAVIAGFEYGRQLLENEKKP"
     gene            790..1749
                     /gene="miaA"
                     /locus_tag="PLW07_03770"
     CDS             790..1749
                     /gene="miaA"
                     /locus_tag="PLW07_03770"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOL49675.1"
                     /translation="MKKNPENLVVIIAGPTGTGKSQVALELSNIIPSEIINADSRQLY
                     REIDVGTAKPPVSALKQTPHHLYSFLSLKDNFSVFDFRKYLEKLVPEIWSREKCAIIT
                     GGSGLYIRAILKGIFDFPHEKKSLQNVVRNQLISQETDQLYEQLKTVDPSIAEKVHPN
                     DRVRITRAIEVWLITGIPMSQWQLQAKPADFIEKAHVFYWILNMKREILYSRLDLRTE
                     AMLESGWLEEVKNLIDTGLKDFLKEKAPIGYLELCDFVEGKMTWDDTVELVKRKTRNY
                     ARRQLTWFRKEKDAQWLDVTDLHPSETTRIIAEKIRETHGKGN"
     gene            1733..3907
                     /locus_tag="PLW07_03775"
     CDS             1733..3907
                     /locus_tag="PLW07_03775"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HOL49676.1"
                     /translation="MEKEIRVDRIFPLKDASFYPGKEFCDIVYFCPPITGKKRLWLLT
                     EAKKPIVYIDHYKFEPRNISKKDKYRWIDYGITEVELAMSRGFGQIKVYGVNPEQAIN
                     SYFVITVQTNKKLSGSIESVEKKLFGITREDTGRSFISPSTVEVGRPVTFKLIYQAGK
                     NGLPEKSYIRFSISRVFTQPQTENSRHDGFLRVVQSDAELIFKGIKGPSDESHRDADI
                     IYFLPDGIRANGKIEIEYHTDTTFICPVTSHTMERRYWYSNMPSLSPAVALDKKKIFV
                     PVSENNGQCVRFVAGKPERLFLFFPGRIREKETIDLVGIITDRYRNIVEDKNWKVKIN
                     ILLEGSQKKEIKNISEHLTHRHRFCIDFHNLVPGIYRAKAFDQDTGKLLAISNPVEIM
                     DASDNRDQIFWGEIHGHTEMSDGIGAFENLYENAKNTGCLDFAAAADHACYFTDNQWE
                     WMQDVTNSFNKPGRFCTIVGYEWAGNQGHRNIYTSENRLKLFRGMYKPAQDIEVVYKT
                     FEGNPSVVAGPHTGHTGDFWKFHNPDVERFLEIYSMWGHFDHLANKLLNEGAIIGFTG
                     GGDCHSGNVFFSPEDPDGQGKTPHFTSYAIKYKCGIMAAFMKTLDRKNLIEALRNRRT
                     YATTSDRILVDFSISGYKMGEMGTDGNIVITADIHGCDIIKKIEVVRNGTVVHVEKPE
                     SMDTMFQWKDSNIERKKYWYYLKITQKNREVAYTGPIWIEVI"
     gene            3923..4456
                     /locus_tag="PLW07_03780"
     CDS             3923..4456
                     /locus_tag="PLW07_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOL49677.1"
                     /translation="MEFCQVIKTRRSIRKYTGKEIDEEVLRRVLDAARIAPSGSNRQP
                     WKFIVVKDSERKNKLAQLCHNQFFIAEAPVVIVACGRNIHYNRGGWMGDCSVLVDVAI
                     AVDHLTLAARAEGLGTCWIGLFDNTGTKKFLEIPDDVNVVALTPLGYPAHRDQFMETS
                     DRLPLEEIVFFEKWGKK"
     gene            4453..>5497
                     /locus_tag="PLW07_03785"
     CDS             4453..>5497
                     /locus_tag="PLW07_03785"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOL49678.1"
                     /translation="MKPNILHIITHDTGRYLECYGAAVKTPNINKLTQNSTTFTNYFC
                     CAPQCSPSRAGMFSSLVPHRNGMMGLAHRGFRLKKGIPYLPEILRNNGYRTILLGIQH
                     EAEWGKHAELGYQDFFTSEITSCIHLGEVLCNILENKPQEPFFISMGVSETHQRYPIV
                     EHVDEQLKLPEFLPDSIEVKKDIAGLNILVQRVDDAIGKIISTLEKTKLMDNTLIIFT
                     TDHGIALPGAKATLFDPGIEIFLIMKGPGIPEGKTINCLSWNIDLLPTVLDYLGMEIP
                     DNIDGVSLMPVLKGQKTSVRECIYPELTYHAGYDPMRSIRTEKYKYIRCFETRSYYFP
                     VNVDESFSKTLFKK"
BASE COUNT         1846 a          936 c         1204 g         1511 t
ORIGIN      
        1 caattgctga agtatgggaa aaaagtgtga tggtgctatg ggaaaaaggc gctcaaatta
       61 agacagaata tgatcaacca gatgaccctt ttagcaggga cgcaacaatg gtgatggttg
      121 tcaatgaacc tttttcagaa ccaagaattc atcttggttt tccaggcggc atggaagacc
      181 ttgaaaaata cagacaggaa gtggttcttg gtgtacacga ccactggata aaacccgaag
      241 aaggcaagtg gacctataca tatcatcaga gattgtttaa ttatcctgtt tcagaaggca
      301 acactgttaa tcaggttgaa tacattattg acaaactctc tcgtgttttt tacacccgca
      361 gggctcaggc aattacatgg gttccttcgt atgaccctga aacagacgac ccaccatgcc
      421 tgcaaagaat ctggtgcaga attgttacgg aaaaagataa tcattatctc agaatgaata
      481 cccactggcg ttcaagggat gcttacaggg cagcatacat gaatttattc ggattaacag
      541 aattacagaa atatatcgca gatgaaattt caaaaagaac agggaaagaa attcttgttg
      601 gtccttatgt tgacattact gatgcatatc atatttatgg ttctaacttc tgtgatttca
      661 aagatagatt tcttaaaatg atggatacca gggattttta caatcaggac agactcaaaa
      721 gcagaaccat gagaagcgat gaccctgcag tcattgccgg ttttgaatac gggaggcaac
      781 ttctggagaa tgaaaaaaaa ccctgaaaat cttgttgtaa ttattgcagg tcctactggt
      841 actgggaaat ctcaggttgc tctggaattg agcaatatta ttccatctga aattattaat
      901 gctgattcca gacagttgta cagggaaatt gatgttggaa cagcaaagcc accagtgtct
      961 gccctgaaac agacaccgca ccatctttac agttttctat cactgaaaga taatttcagc
     1021 gtatttgatt tcagaaagta ccttgaaaaa cttgtgcctg agatatggtc aagggaaaaa
     1081 tgcgcaatca ttactggtgg cagtggtctg tatatcaggg cgatacttaa aggaattttt
     1141 gattttcccc atgaaaagaa atcccttcag aatgttgtca gaaaccagtt gatttcacag
     1201 gaaacagacc agttatacga gcaattgaaa actgttgacc cttctatcgc tgaaaaggta
     1261 catccaaatg acagggtaag aattaccagg gcgattgaag tgtggttgat aacagggatt
     1321 ccaatgagtc agtggcaatt gcaggcaaaa cccgcagatt ttatagaaaa agcacatgtg
     1381 ttttactgga tactgaatat gaaaagagaa attctttata gccggcttga tttgagaaca
     1441 gaagccatgc ttgaatctgg ttggctggaa gaagtgaaaa atcttattga tacagggcta
     1501 aaagattttt tgaaggaaaa ggcgccgatt ggatatttgg agttgtgcga ttttgttgaa
     1561 ggtaaaatga cctgggacga cacggttgaa cttgttaaaa gaaaaacgag aaactacgca
     1621 agaagacaac tgacatggtt cagaaaagaa aaagacgcgc aatggctgga tgttacggat
     1681 cttcacccat ctgaaacaac aaggattatt gcagaaaaaa taagggagac gcatggaaaa
     1741 ggaaattaga gttgacagga tttttccact gaaagatgca agtttttatc cagggaaaga
     1801 attttgtgac atagtgtatt tttgtccacc cattactgga aagaaaagat tgtggctttt
     1861 gactgaagcg aaaaagccaa ttgtttatat tgaccattat aaatttgaac ccaggaatat
     1921 ttcaaaaaaa gacaagtaca gatggattga ttatggtatc actgaggtgg aacttgctat
     1981 gtcacgcggt tttggacaga taaaagttta tggagtcaat cctgagcagg caatcaattc
     2041 ctattttgtg atcacagtcc agacaaataa gaaattgtct ggcagcattg aaagtgttga
     2101 aaaaaaactt ttcggtatta caagggaaga tactggtaga agttttataa gtccttcaac
     2161 cgttgaggtg ggaagacctg tcacattcaa acttatttac caggcaggaa aaaacggact
     2221 gccagaaaaa agttatatca ggttttctat ttcaagggta tttacccagc cacaaacaga
     2281 aaacagccgt cacgacggat ttctcagagt tgttcagtct gacgcagaat tgattttcaa
     2341 aggcattaag ggtccttctg atgaatccca cagggatgca gacattatct actttctgcc
     2401 cgatggaata agagcaaacg gaaaaattga aattgaatac cacactgata ctacatttat
     2461 atgtcctgta acaagccata caatggaaag aagatactgg tattcaaata tgccttcatt
     2521 atctcctgct gttgcccttg ataagaagaa aatctttgtg cctgtgtcag aaaacaatgg
     2581 acagtgtgtt cgatttgttg caggaaaacc tgaacggttg ttcttatttt ttcctggcag
     2641 gataagagag aaagaaacaa tagaccttgt cgggattatt accgatagat acaggaacat
     2701 tgtagaagat aagaactgga aggtaaaaat aaacatactt cttgaaggat cacagaaaaa
     2761 agagataaaa aacatttcag aacatcttac acacagacac agattttgca ttgattttca
     2821 caatctcgta ccaggtattt acagggcaaa ggcatttgac caggatacag gaaagcttct
     2881 tgctatcagc aatcctgttg aaattatgga cgcaagcgat aatagagacc agattttctg
     2941 gggtgagata cacggtcata ctgaaatgag tgacggtata ggcgcgtttg agaacctgta
     3001 tgaaaatgca aaaaatacgg gctgtcttga ttttgctgca gcagcagatc atgcctgcta
     3061 tttcactgat aaccagtggg aatggatgca ggatgttacc aacagtttca acaaacccgg
     3121 ccgattttgc acaattgttg gatatgaatg ggcaggaaat cagggacaca gaaacatcta
     3181 tacttcggaa aacaggctta aactttttcg gggaatgtat aaaccagcac aggatattga
     3241 agttgtctat aagacatttg aaggtaaccc atcagttgtt gctggaccac atacaggaca
     3301 taccggagat ttctggaaat ttcataatcc tgatgttgag agatttcttg aaatttattc
     3361 aatgtgggga cattttgacc atcttgccaa caaacttttg aacgaaggtg ctatcattgg
     3421 ttttacaggc ggtggtgatt gtcatagtgg aaacgtgttt ttctcacctg aggacccaga
     3481 tggccagggt aaaacaccgc attttacatc atatgcaata aaatacaagt gtgggattat
     3541 ggctgctttt atgaaaaccc ttgataggaa aaaccttatt gaggcattga gaaacaggcg
     3601 aacatatgca acaacaagcg ataggatcct tgttgatttt tcaatttccg ggtataaaat
     3661 gggagaaatg gggacagatg gtaacattgt gattacggct gatattcatg gctgtgacat
     3721 cataaaaaaa attgaagttg taagaaatgg aacagttgtt catgtggaaa aacctgaaag
     3781 catggacaca atgtttcaat ggaaggatag caatattgaa agaaaaaaat actggtatta
     3841 tctcaaaatt actcagaaga atagagaagt tgcatataca ggcccaatct ggatagaagt
     3901 aatttgaaaa caggagggaa acatggagtt ttgtcaggta ataaaaacaa gaagaagtat
     3961 tagaaaatac acaggcaaag aaattgatga ggaggtatta agacgtgttc ttgatgcagc
     4021 aagaattgct ccaagtggaa gtaatagaca accatggaag tttattgttg ttaaagacag
     4081 tgaaagaaaa aacaaactgg cacaattatg ccacaatcag ttttttattg cagaggcacc
     4141 tgttgtgatt gttgcatgcg gaagaaatat tcattataac aggggtggct ggatgggtga
     4201 ttgtagcgtc cttgttgatg tggcaattgc tgttgaccat ttgacccttg ctgcgagagc
     4261 agaaggtctt ggcacctgct ggattgggtt atttgataat acaggaacaa aaaaattcct
     4321 tgaaattcca gatgatgtca atgttgtggc attgactccg cttggttatc ctgcacatcg
     4381 agatcaattt atggaaactt cagacagatt accccttgaa gaaatcgtat tttttgaaaa
     4441 atggggcaaa aaatgaaacc caatattctt catatcatta cccatgatac tggaaggtat
     4501 ttagaatgtt atggtgctgc tgtaaaaaca ccgaacatca ataaacttac acaaaactca
     4561 acaactttta ccaattactt ctgttgtgct ccacaatgca gtcccagcag ggcaggaatg
     4621 ttttcatctc ttgtgccaca cagaaatggt atgatgggtc ttgcccacag gggttttaga
     4681 ttaaaaaaag gtattccata tcttcctgaa attcttcgca acaatggtta tcgtacaatc
     4741 ctgcttggta tccagcatga agcagaatgg ggaaagcacg ctgaactggg gtatcaggat
     4801 tttttcacat cagaaatcac atcatgtatt caccttggag aagtgttgtg taatattctc
     4861 gagaataagc cacaggaacc attttttatc tcaatgggcg tttcagaaac ccatcagaga
     4921 tacccgatag ttgaacatgt agatgaacaa ctgaaacttc ctgagtttct tccagatagc
     4981 attgaagtga aaaaggacat agcaggtctt aatatacttg ttcaaagggt tgatgatgcc
     5041 attggaaaaa tcatttccac ccttgagaaa acaaaactga tggataatac acttattatt
     5101 ttcaccacag accatggaat tgcactgcct ggcgcaaagg caacactttt tgacccaggg
     5161 attgaaattt tcctgattat gaaaggacca ggaataccag aagggaaaac cattaattgc
     5221 ctttcctgga acatagattt actgccaaca gttcttgatt atctcggaat ggagattcct
     5281 gataatatcg atggggtcag tttgatgcct gttttgaaag ggcagaaaac cagtgtaagg
     5341 gaatgtattt atccagaact tacatatcat gctggatatg acccgatgag gagcatcagg
     5401 acagaaaaat acaagtatat tcgttgtttt gaaacacggt cttattattt ccctgtcaat
     5461 gttgatgaat ctttttccaa aactcttttt aaaaaat
//
LOCUS       DAORYU010000084         5440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_10256_length_5440_cov_5.197957, whole genome shotgun sequence.
ACCESSION   DAORYU010000084 DAORYU010000000
VERSION     DAORYU010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5440
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10256_length_5440_cov_5.197957"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="PLW07_03790"
     CDS             complement(<1..474)
                     /locus_tag="PLW07_03790"
                     /inference="COORDINATES: protein motif:HMM:NF014034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HOL49679.1"
                     /translation="MKHINKFGDADIAKALLSRIYMEAKSIKKAVNLMEVCGTHTMAI
                     SRYGLRGLLPENIKLISGPGCPVCVSPNSFIDKAIAISKIPGMRIATFGDMYRVPSAM
                     SSLEKEHGCGASIDIVYSPLDALEIARSHSNEKVVFLGVGFETTAPLVANVIKYAK"
     gene            complement(461..682)
                     /locus_tag="PLW07_03795"
     CDS             complement(461..682)
                     /locus_tag="PLW07_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HOL49680.1"
                     /translation="MCLAVPMKVISVKGKKAFADLGGVKREIDISLVKNIKVGDYIIV
                     HAGFAIQKLNKKEALNTLELFKQIKNETY"
     gene            complement(696..2993)
                     /gene="hypF"
                     /locus_tag="PLW07_03800"
     CDS             complement(696..2993)
                     /gene="hypF"
                     /locus_tag="PLW07_03800"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HOL49681.1"
                     /translation="MKRIEIIITGVVQGVGFRPFVYRVARKHNIRGFVKNTIAGLIIE
                     AEGKIENVENFLKEIKEGPPVSARIKSVYVRNISVKNSKNFLITKSNGDLIEKPEIPP
                     DIAICPQCRNEIIDSKNRRFWYPFTNCVDCGPRFTIIDKLPYDRENTTMRSFKMCKEC
                     LEEYKNPENRRFHAQTNCCEKCGPQVFLTDRHGRRICESRNAIEQAGYFLLNAKIVAV
                     KGIGGFHLACDAVSITAVKNLRTRKGREEKPFALMARDIETIRKYCELSDTEIDYLVS
                     PAAPIILLKKKGQKRWFDYVAPGNKYLGVMLPYSGIHQLIFESNPDIELLVMTSGNIS
                     EEPICIDNEEAIKRLSGIADYFLFHDRTISSGCDDSVMRIFPDRNVLITRRSRGYAPE
                     SMMLPFTAKKQILACGADNKNVFGLVSKNNLYLSHHIGDLEHVLNFESYRRAIEKYVS
                     ILKIKPDLVVYDAHPEYFSSSLAMSDEFFKEAKKIPIFHHHAHVCSSMIENHLPDKPV
                     LGLAFDGTGYGADGTLWGSEFLLSDYKHFQRLAHLKYVRLPGGERAIKQIWRIAVSYL
                     VDAFDGEIPAKFFKNIDANKVSFISDMIKKGINSPYACGLGRIFDAVSCLCGIRNEAN
                     YEGQAAIELEAMLTEGRNDTPVSYDFDITRESGQIVIDHKKIIRGVVDDLLGGKDAGS
                     VSYKFHLTVVKFTVDVCHLLREQTHISDVVFSGGSFQNVFLYTNIKRMLEKDGFKVYS
                     HKIIPTNDGGIAAGQCAIANFTNKP"
     gene            complement(2980..3651)
                     /gene="hypB"
                     /locus_tag="PLW07_03805"
     CDS             complement(2980..3651)
                     /gene="hypB"
                     /locus_tag="PLW07_03805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HOL49682.1"
                     /translation="MSLKKIDVGENLFAKNDEVAAENKKTLKEHNIFSINLMGGPGSG
                     KTAILEKLVPEMKKRKIFCGVIEGDIAGTFDGERISRLGVPVVQINTGGACHLEAMMI
                     KKGMENLPFSEINVLFIENVGNLVCPAEVKLGVDCNVTVSSITEGEEKPAKYPLMYSI
                     SDVVLINKIDLSEVIEFNMDRFTGYINKIKSEIVIISVSGKTGENIDVFADYIEKRVL
                     NNEKD"
     gene            complement(3666..3926)
                     /locus_tag="PLW07_03810"
     CDS             complement(3666..3926)
                     /locus_tag="PLW07_03810"
                     /inference="COORDINATES: protein motif:HMM:NF013334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase/urease maturation nickel
                     metallochaperone HypA"
                     /protein_id="HOL49683.1"
                     /translation="MHEMSVIENLVLLLRDVCAKEKAIRVVLVHMTIHPFSCLDEGNL
                     NFMFSCIVGDDPLLKGAKIKVQRNKNLQEPEYIVDNVELELE"
     gene            complement(3958..4461)
                     /locus_tag="PLW07_03815"
     CDS             complement(3958..4461)
                     /locus_tag="PLW07_03815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation endopeptidase HyCI"
                     /protein_id="HOL49684.1"
                     /translation="MELWKKQFIQKLNNFHKLVIMGIGNPESSDDGLGPEIVNIIAER
                     ITKNYSSVAVINCYGSPENFTSKIKKENPTHILIIDSCIAGKEPGSIFLVKPSQIKNM
                     DLSTHRLPLYLLHDYLKSETGAKIIMLGIEPQSVCPGTSFSTPVRQAIGETVEFLLDF
                     LKIYHKE"
     gene            complement(4480..4884)
                     /locus_tag="PLW07_03820"
     CDS             complement(4480..4884)
                     /locus_tag="PLW07_03820"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOL49685.1"
                     /translation="MKFKPAAFVPELLRHLFRRPATIEYPFKKMKVPDDFRGTPILKP
                     ELCIVCRACVRDCPAFAIEIETLSDEEKRYKMTIHNDRCIHCAQCVESCPTKALFMND
                     EFEIATDSRLKLKAEYTYTRPAKKSTQQSQNG"
     gene            complement(4881..>5440)
                     /locus_tag="PLW07_03825"
     CDS             complement(4881..>5440)
                     /locus_tag="PLW07_03825"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOL49686.1"
                     /translation="FSTLGGVRVLTMLFGYEVPLFLAILSPAIVADSWRLAEISYFYQ
                     KFPFLLLFQVTGFFVSVIALQAKLERTPFDIPDAETEIVGGTFTEYSGKKLAFFRLMT
                     DIEMVVGSGLIATVFMAGFSGGIVLGLFKFVLKTLLVIAILSLIRAACSRIRVDQMVV
                     FSWKYLTPLAVLQLLISIVVKGIIQ"
BASE COUNT         1674 a         1197 c          831 g         1738 t
ORIGIN      
        1 ttttgcatat ttaataacgt ttgcaacaag aggcgctgtt gtttcaaaac caactcccag
       61 aaacactact ttctcatttg aatgacttct ggcaatttcc agtgcatcga gaggtgagta
      121 aacaatatca atgctggcgc cacaaccatg ttctttttca agcgaagaca tggctgaagg
      181 aactctgtac atgtcgccaa atgttgcaat cctcatacct ggaatttttg aaatagcaat
      241 ggctttatca ataaatgaat ttggacttac acacacagga cagcctggac ctgaaatgag
      301 tttgatattt tctggcaata atccacgcaa gccatagcgc gatatcgcca ttgtatgagt
      361 accacaaacc tccatcaggt ttactgcttt tttaatactt tttgcctcca tatatattct
      421 ggaaagcaat gccttagcaa tatctgcatc accgaattta ttaatatgtt tcatttttta
      481 tttgcttaaa aagttccaag gtgtttaacg cttccttttt attaagtttc tgaatcgcaa
      541 atcctgcgtg tacaatgatg tagtcaccaa ctttaatgtt tttaaccaga gatatatcaa
      601 tctccctttt tacccctcca aggtctgcaa acgctttttt ccctttcacg cttatcactt
      661 tcatcggcac agcaagacac atacttcccc cttcattagg gtttgttcgt aaaattcgca
      721 atagcacact gtcccgcagc aattcctccg tcattggttg gaattatttt atgggaatac
      781 actttgaaac catctttttc aagcattctc ttgatgttag tataaagaaa aacattctga
      841 aaactcccgc cagagaacac aacatccgaa atatgggtct gctcgcgcag caaatgacag
      901 acgtcaacag taaattttac cacagtcagg tgaaacttat aagaaacaga acccgcatct
      961 tttcccccca ataaatcatc aacaacacca cgaataattt tcttatgatc aattacaatt
     1021 tgccctgact ccctggtgat atcaaagtca taagaaacag gcgtgtcatt tcttccttct
     1081 gttaacattg cttcaagctc aatcgctgcc tgaccttcgt aattcgcttc atttcttatc
     1141 ccacacagac aactgactgc gtcaaaaatc cttccaagtc cgcaggcata cggactgtta
     1201 attccttttt ttatcatatc tgaaataaaa gatactttat tagcatcaat attcttgaaa
     1261 aactttgcag gaatttctcc atcaaaagca tccaccagat atgatactgc aatcctccag
     1321 atttgtttga ttgctctttc tccaccagga agtcgaacat attttagatg tgctaaacgt
     1381 tgaaaatgtt tgtaatcgga taataaaaat tcacttcccc acaaagtacc atctgcacca
     1441 taccctgttc catcaaaagc aagacccagc actggtttgt ctggcagatg attttctatc
     1501 atagacgaac atacatgcgc atgatgatga aaaattggta ttttcttcgc ttctttaaaa
     1561 aactcatctg acatcgccag agaagaagaa aaatactcag gatgagcatc atatactact
     1621 aaatctggtt ttattttcag tatgcttaca tatttttcaa ttgctcttct gtacgattca
     1681 aagttcaaaa catgttcaag atctccgata tgatgactca gatacaaatt gtttttcgat
     1741 accaaaccaa atacattttt attatctgcc ccgcatgcca gaatctgttt ctttgcagta
     1801 aaaggcagca tcattgattc aggagcataa cctctcgacc ttcttgttat cagcacatta
     1861 cgatcaggaa aaattctcat cacagaatca tcacacccgg atgaaattgt tctgtcatga
     1921 aaaagaaaat agtcagcaat accagaaagt ctttttattg cttcctcatt atcaatgcaa
     1981 ataggttcct cggaaatatt tccacttgtc attaccaata attcaatatc tggatttgac
     2041 tcaaaaatca actgatgtat cccggaataa ggcaacatta caccaagata tttatttccg
     2101 ggagcgacat aatcaaacca gcgcttctga ccttttttct tcaaaagaat aatgggtgct
     2161 gcaggagaaa caagataatc aatttctgtg tcactaagtt cgcagtactt tctgattgtt
     2221 tcaatatccc gcgccattaa agcaaagggt ttttcttctc taccttttcg agtgcgaagg
     2281 ttctttacag cagttattga cacagcatcg caggcaagat gaaaccctcc aattccctta
     2341 acagcaacaa ttttagcatt gagcaaaaaa taccctgctt gctctattgc attccttgac
     2401 tcacagattc gcctgccatg acgatccgtc aaaaacacct gaggtccaca cttctcacag
     2461 cagttggtct gtgcatgaaa ccttctgttt tcaggatttt tgtattcttc caggcattcc
     2521 ttacacattt taaaacttct cattgtagta ttttctctat cgtatggcag tttatcaatt
     2581 attgtaaaac gtggaccaca gtcaacacaa tttgtaaaag gataccagaa cctgcgattt
     2641 tttgaatcaa tgatttcatt acgacattga ggacatatcg cgatatcagg tggtatttcc
     2701 ggcttttcga taagatcccc gttacttttt gttatcagaa aatttttcga atttttaact
     2761 gaaatatttc tcacatatac tgatttgatt cttgcactca caggaggtcc ttctttgatt
     2821 tccttcaaaa aattttcaac attttcaatc ttgccttctg cctcaataat aagtccggcg
     2881 attgtatttt ttacaaatcc tctgatatta tgttttctgg caacacgata aacaaaaggt
     2941 ctgaaaccaa ctccctgaac cacacccgta ataatgattt caatcctttt cattgtttaa
     3001 tacgcgtttt tcaatgtagt cagcaaaaac atcaatattt tctcctgttt tcccggatac
     3061 agaaatgatc acaatctccg attttatttt gttgatataa ccggtaaatc tatccatatt
     3121 gaattcaatt acttcagaaa ggtcaatttt attaataaga acaacatcag aaatggaata
     3181 cattaaggga tatttggctg gtttttcttc tccctctgta atgcttgaaa ctgtcacatt
     3241 gcagtccaca ccgagtttga cttcagccgg acatacaagg ttcccaacgt tttcgataaa
     3301 gagcacattt atttcagaaa agggtaaatt ttccatccct tttttgatca tcattgcttc
     3361 aagatgacat gcaccaccag tgttaatctg cacaacaggg acacccaatc gggaaatccg
     3421 ctcaccatca aatgttcctg caatatctcc ctcaataaca ccacaaaaaa tttttctttt
     3481 tttcatttct ggaaccagtt tttcaagaat cgctgttttt ccagacccgg gaccgcccat
     3541 aagattaata gaaaaaatat tatgttcctt cagggttttt ttattttctg cagccacctc
     3601 atcgtttttt gcaaacaaat tttcgcctac atcaattttt tttaaactca tgacatctcc
     3661 ttgatttatt caagttcaag ttccacatta tccacaatat attctggttc ctgtaaattc
     3721 ttatttctct gtactttaat ttttgcaccc tttaacaaag ggtcatcccc gacaatacag
     3781 gaaaacataa aattgagatt cccttcgtca agacagctga acggatgtat ggtcatgtga
     3841 accaatacaa cccttatggc tttttccttt gcacaaacat cccttaataa taatacaaga
     3901 ttctcaataa cactcatctc atgcattgtt tccaccggtc ggaacttgca ttatatttta
     3961 ctctttatgg tagattttta gaaaatccag cagaaattca acagtttctc caatcgcctg
     4021 ccttactgga gtactgaaag acgttccagg acacacggat tggggttcga tacccagcat
     4081 aataattttt gcgccggttt cacttttaag ataatcatgt aagagataaa gtggtagcct
     4141 gtgggttgac aagtccatat ttttaatctg agatggcttt acaagaaaaa tcgaacctgg
     4201 ttctttacct gctatacagg aatctataat aagaatatgc gttggatttt ccttttttat
     4261 tttagaggta aaattttcag gagacccata gcaattgata acagcaacac tcgaataatt
     4321 ttttgtaata cgttctgcaa taatatttac aatttcaggt ccaagtccat catcagaaga
     4381 ttcagggtta ccaataccca ttatgaccag tttatggaaa ttattcaatt tctgaataaa
     4441 ttgtttcttc cacaattcca taggtataat agacgattat tagccgtttt gcgattgctg
     4501 ggttgatttt tttgctggtc gggtgtatgt gtattcggct ttcagtttca atcggctgtc
     4561 tgttgcaatt tcaaactcat cgttcataaa taatgccttc gtcggacacg actccacaca
     4621 ttgtgcacaa tgaatacatc tatcattgtg aatcgtcatt ttatatcttt tctcttcatc
     4681 tgaaagtgtt tcaatttcaa tcgcaaatgc gggacaatcc cttacgcacg ctctgcagac
     4741 aatacatagt tcaggtttta aaatcggtgt acccctgaaa tcatcaggaa ccttcatttt
     4801 cttgaatgga tactctattg tggctggcct tctaaaaaga tgccttaaca attcaggtac
     4861 aaatgctgct ggtttgaatt tcattgtatt atccccttta caacaatact tattaacaat
     4921 tgcagaacag caagtggagt taaatacttc caggaaaaaa ccaccatctg atctaccctg
     4981 attcttgaac acgctgccct tatcaaagaa aggattgcaa tcacaagcaa tgtcttcaag
     5041 acaaatttaa ataatcccaa aacaatacca ccagaaaaac ctgccataaa aacagtcgca
     5101 atcaaaccgc ttccaacaac catttcaatg tctgtcatca atctaaaaaa tgcaagtttc
     5161 tttcctgaat attctgtaaa agttcctcca acaatttctg tttcagcatc aggaatatca
     5221 aaaggagtcc tttcaagttt tgcctgaagt gcaatcaccg agacaaaaaa accagtgacc
     5281 tggaacaaaa gaagaaaagg aaatttctga taaaaataag atatctcagc aagtcgccag
     5341 gaatctgcaa cgattgctgg acttaaaatt gcaagaaaca aaggtacctc atacccgaaa
     5401 agcatcgtta agacccttac ccctccgagt gttgagaaaa
//
LOCUS       DAORYU010000085         5424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_10298_length_5424_cov_4.427454, whole genome shotgun sequence.
ACCESSION   DAORYU010000085 DAORYU010000000
VERSION     DAORYU010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5424
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10298_length_5424_cov_4.427454"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..445
                     /locus_tag="PLW07_03830"
     CDS             59..445
                     /locus_tag="PLW07_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOL49687.1"
                     /translation="MRFAISTDGDFVSPHFGRCPSFTVVDIENNKVIKIEVLENPGHH
                     PGYLPEFMHKKGVSSIVCGGMGQRAKDLFQQLGIQTIVAVKGKINDVIEKLLKGELKS
                     GESLCKPGAGKNYGLEKTECDHQGGD"
     gene            489..662
                     /locus_tag="PLW07_03835"
     CDS             489..662
                     /locus_tag="PLW07_03835"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49688.1"
                     /translation="MTGRGQGRGAGRRMGGRGAGPGGFCVCPSCGEKVVHQAGVPCFE
                     INCPKCGTRMVRG"
     gene            667..1539
                     /locus_tag="PLW07_03840"
     CDS             667..1539
                     /locus_tag="PLW07_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOL49689.1"
                     /translation="MIITVASGKGGTGKTLVSTSLALSLGKDAVFLDCDVEEPNGYIF
                     LKPDITENIKIYLPQPGVDIEKCNFCGKCASACLFNAIAVIKPETGKRNGNVLFFHEM
                     CQSCGACVLVCPQKAIYEKRSEKGEVEKGVTKNGVSFVQGRLKPSEAKSVPLVKAVKK
                     HVNVNKINIIDSSPGTSCPVVQTVKDSDFCLLVTEPTPFGLNDLRLAVEMTRVLAVPV
                     GVVINRFDIGDNSVQEYCLKENLPVLLSIPFKKDIAVAYSKGIPLIESFPEYRKIFKK
                     LFKDISDIIVQGKK"
     gene            1544..2425
                     /locus_tag="PLW07_03845"
     CDS             1544..2425
                     /locus_tag="PLW07_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013756982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOL49690.1"
                     /translation="MKKILVISGKGGTGKTFLTASLSYLASDKKIIVDCDVDAANLYL
                     LLHPDIKRSEVFEWGYEAVINQDKCIRCGKCLAICRFEAIKTVHKDDNRGIDRITVDP
                     FSCEGCGVCKYGCPVGAVDLRKKESGKWFISDTKYGPFVFAKLGIAAENSGKLVSKIK
                     QEAYTLAEKELAEYIIIDGPPGIGCPVIASMSGVDLALVVTEPTLSGISDMKRVMEVA
                     EHFQVETKVVVNKYDINLETTEEIRRICYAKGIEVLGQLPFSEKVVQSIVKQVPFTEF
                     SNGSIAKDIAKIWEKIK"
     gene            complement(2855..2939)
                     /locus_tag="PLW07_03850"
     tRNA            complement(2855..2939)
                     /locus_tag="PLW07_03850"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2904..2906),aa:Leu,seq:taa)
     gene            complement(3082..3795)
                     /locus_tag="PLW07_03855"
     CDS             complement(3082..3795)
                     /locus_tag="PLW07_03855"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOL49691.1"
                     /translation="MEIKILFDSNAIDSKFQTGWGFSCLINGRILFDTGEKGEWLIHN
                     IKQMNIDINNIQSIVISHDHWDHTGGLWDVLQLKKNLPVYSCPDFSKNLKENVRKLKG
                     IFIESNHFQQIEENISVTGEIEATYNGKTISEQAVVIKTKNGISIITGCAHPGILNIV
                     KIAKQHFPDEQVFLVMGGFHLLDADTRIIEYIATELKTMNVNTVSPCHCSGKNAEEIF
                     QQIFQKNFLAVKAGMTVKV"
     gene            complement(3796..3990)
                     /locus_tag="PLW07_03860"
     CDS             complement(3796..3990)
                     /locus_tag="PLW07_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49692.1"
                     /translation="MPGRNGTGPDGRGPGMRRGGRRQQFAGPVENCICPICKTKVPHQ
                     PGVPCSSITCPECGTPMTRE"
     gene            complement(4090..4464)
                     /locus_tag="PLW07_03865"
     CDS             complement(4090..4464)
                     /locus_tag="PLW07_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HOL49693.1"
                     /translation="MPWGDGTGPIGAGPMTGRGAGFCAGYNVPGYMNPYGGIWMRGAF
                     GRGRGFRHWYRATGLPGWVRANMGLPAWGWGTPYAPFTGEITPQQEADILKKQAEAIK
                     KELESIEKRIATLEKIAAEEKK"
     gene            complement(4570..5160)
                     /locus_tag="PLW07_03870"
     CDS             complement(4570..5160)
                     /locus_tag="PLW07_03870"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOL49694.1"
                     /translation="MKCGKRSFFEKHAQHWDNHPEPKNLKRVVEEVEIKKGSMICDVG
                     TGTGVLVPYLLEKTGKKGKVIAIDYAPAMIEQAIRKLRHLPVEFIIADIHHTCFPDNY
                     FDYVICNACYPHFDRKKTAIKEIYRILKKDGSIVISHPTGKDFVNNLHKSTGGCIEKD
                     RVPEAKILAKTLASHGFSVLKIIDEPDFYFVSGKKI"
     gene            complement(5141..>5424)
                     /locus_tag="PLW07_03875"
     CDS             complement(5141..>5424)
                     /locus_tag="PLW07_03875"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOL49695.1"
                     /translation="ATITSSIKAVGGLLVFALIVNPAAAAYQLTYDLKKMFLISSIFG
                     AASGCLGLLVAFWLNIPAGAPIALCSVIIFIIATIFSPKKRMVYEMRKA"
BASE COUNT         1572 a         1055 c         1183 g         1614 t
ORIGIN      
        1 aacggtatca tcccattttg ggatgggcgg taaaaaacaa taaatctgga ggtgcaatat
       61 gcgttttgct atttctaccg acggagattt tgtgtcgccc catttcggaa gatgcccgtc
      121 tttcacagtt gtggacattg aaaacaataa ggtgataaaa atagaggtac tggaaaaccc
      181 gggacatcat ccaggttacc tgccggaatt tatgcataaa aaaggtgtga gttctatcgt
      241 atgcggcggg atgggtcagc gtgcaaaaga cctctttcag cagttaggga tacagacaat
      301 tgtggctgtt aaagggaaaa tcaacgatgt aatagaaaaa ctgttgaaag gtgaactaaa
      361 atcaggtgag agtttgtgta aacccggtgc aggcaagaat tacggtttgg aaaaaacaga
      421 atgtgatcat caagggggtg attaaagtgc cacgaggaga tggaacagga ccaatgggtt
      481 tgggtccaat gacaggcaga gggcaaggaa gaggagctgg aagaagaatg ggaggcagag
      541 gcgcaggtcc cggaggtttt tgcgtatgtc catcatgcgg tgagaaggtt gtacatcagg
      601 caggtgtccc ctgttttgag ataaattgcc ccaaatgtgg aacaagaatg gtaaggggat
      661 aatagaatga taataacggt agccagcgga aaaggcggta ccggcaaaac tcttgtttcc
      721 acatcacttg ctttatcctt gggaaaagac gctgtgtttc ttgactgtga tgtcgaagaa
      781 cccaacggct atattttttt gaaaccagat ataacagaaa acataaagat ttatctgccc
      841 cagccagggg tagatattga gaaatgtaat ttttgtggca aatgcgcttc tgcatgctta
      901 ttcaatgcaa tagccgttat caaaccagaa acaggcaaga gaaatggtaa tgttctcttc
      961 ttccatgaga tgtgccagag ctgcggtgct tgcgtgctgg tttgtccaca aaaagcaata
     1021 tacgaaaagc ggagtgaaaa aggagaggtg gaaaagggtg ttacaaaaaa cggagtttct
     1081 tttgttcaag gacggctaaa accatctgag gcaaaatctg ttcctcttgt aaaggcggta
     1141 aaaaaacatg taaatgtcaa taaaataaat atcattgact cttcgccggg caccagctgc
     1201 cctgtggtcc agacagtcaa ggattcggat ttttgtcttc tggttacaga acccacgccg
     1261 ttcggattaa atgaccttcg tctggcagtg gagatgacca gagtgctcgc cgtgcctgta
     1321 ggtgttgtaa ttaaccgttt tgacatcgga gataattcag ttcaagaata ttgcttgaaa
     1381 gagaatcttc cggttctcct gagtattcca ttcaaaaaag atattgccgt tgcatactca
     1441 aaagggatac ctctgattga atctttccct gagtatagaa aaatattcaa gaaacttttt
     1501 aaagacatat cagatattat cgttcaaggg aaaaaataga aagatgaaga aaatacttgt
     1561 aatcagtgga aaaggcggaa caggaaaaac attcctcaca gcctctttat cttatttagc
     1621 gagcgacaaa aagattatag tagattgtga tgttgatgcc gctaaccttt atctgctatt
     1681 gcatccggat ataaaacgaa gtgaagtatt tgagtggggc tatgaggcgg taattaacca
     1741 ggataagtgc atacggtgtg gtaagtgtct ggcaatatgc agatttgaag caatcaaaac
     1801 cgtacataag gatgacaaca gagggattga tagaattact gtagacccgt tttcctgcga
     1861 aggttgtggc gtatgcaagt acggatgtcc tgtgggagcc gtagacctca ggaaaaaaga
     1921 gagcggcaag tggttcatat ccgatacaaa atatgggccg ttcgtatttg caaaactggg
     1981 tattgcggcg gaaaattcgg gcaaactcgt atcaaaaatc aagcaagaag catatacgct
     2041 tgcggaaaaa gaactggctg aatacattat catagacgga ccgccgggga tagggtgccc
     2101 tgtcattgct tccatgtcag gtgttgacct tgcacttgtg gtgacagagc cgacactctc
     2161 tggcatcagc gacatgaaaa gggtaatgga agtagcggaa cattttcagg ttgaaacaaa
     2221 ggttgttgta aacaagtatg atattaattt ggaaactacc gaggaaataa gaaggatttg
     2281 ctacgctaaa ggcatagaag ttttaggaca actgcctttt tcagaaaaag tagttcaatc
     2341 tatagttaaa caggttcctt ttactgaatt cagcaatggc tccatagcca aggatattgc
     2401 caagatttgg gaaaagatta aataaaaaaa gcaagcggat atgagaaaat tttctatact
     2461 gctccccatt aagggtagat ggaaaataaa gtagaataga gtgatctgaa aggggaagca
     2521 ggacaacatc acatccattt ttgtaaaatc atctcatact atttcccctc tgtataaaat
     2581 tcctgcatac cacccccctc tttataaaag aggggttggg ggagatttca aaatccccct
     2641 taatccccct tttgtaaagg gggagaatta aaggaggaat tttgaaaaga gagaaaattt
     2701 tactgttacc ccttcaggaa gagtgctatg ccgggagagg gattcgagcg gcctcgtctc
     2761 ccactacgac tcgggtcggc cattcgcaca agtcctcgct tgctcggact tatacgtctt
     2821 cgctcaagtt cgaatccctg caaggagacc gcaatgccgg gagagggatt cgaaccctca
     2881 cggaaattac tttccaacgg attttaagtc cgtagcgtct gccattccgc catcccggct
     2941 tattaaaact attataacct gtctggctta aaatttgaaa tttttatcaa gaaaataggt
     3001 attcactgca tttcttagtg ttctgatatc agcagatttg tacggttttt gcggaaaatt
     3061 tgatattttc aattactatt tttaaacctt cacagtcatg cctgctttta cagcaagaaa
     3121 gtttttctgg aagatttgtt gaaaaatttc ttctgcattt tttcctgaac aatggcaggg
     3181 acttactgtg tttacattca ttgtttttaa ttcagtggcg atgtattcaa tgattcttgt
     3241 atcagcgtca agcaggtgga aaccgcccat aacgagaaag acctgttcat cagggaaatg
     3301 ttgcttcgca attttaacaa tattcagaat tcctggatgc gcacatcctg taatgatgga
     3361 aattccattt tttgttttta ttacaactgc ttgttctgat attgtctttc cattgtatgt
     3421 tgcttctatt tctcctgtaa cagaaatatt ttcttcaatt tgctgaaaat gattggattc
     3481 aatgaaaatt cctttgagtt ttcttacatt ttcttttaaa ttcttgctaa aatccgggca
     3541 actgtaaaca ggcaggttct tttttaattg taaaacgtcc cacagtccac ctgtgtggtc
     3601 ccagtggtcg tgagagatga caatagattg aatattatta atatcaatat tcatttgttt
     3661 aatgttgtga atcagccatt cgcctttttc tcctgtgtca aataaaatcc tgccatttat
     3721 caggcaggaa aaaccccagc cggtttggaa tttgctatct attgcattgc tatcaaataa
     3781 aattttgatt tccatttatt ctcttgtcat aggtgtcccg cattctggac aggttattga
     3841 agagcagggg actcctggtt gatgtgggac ttttgtttta cagataggac aaatacagtt
     3901 ttctactggt cctgcaaact gttgtctcct tccacctctt ctcattccag gaccccttcc
     3961 atcaggtcct gttccatttc ttcctggcat cttttatcac ctcttttttt gttgcaattt
     4021 atttatatta ctttttccct gcagagcgtg agccctcctg cagggtttac tgcaggagga
     4081 ctcattcaat tatttttttt cttcagcagc aatcttttcc agtgttgcaa tgcgcttttc
     4141 aatggattct aattctttct tgattgcctc tgcctgcttt ttcaaaatat ctgcttcctg
     4201 ctggggtgta atttctcctg tgaatggtgc gtatggtgta ccccagcccc atgcaggaag
     4261 acccatattt gccctcaccc agccaggcag acctgttgcc ctgtaccagt gtctgaatcc
     4321 tctacctcta ccaaaggcgc ctctcatcca gattccacca tacggattca tataaccagg
     4381 tacgttgtaa ccagcgcaaa atcctgcccc tctgccagtc atgggccctg ctccaatagg
     4441 tcctgttcca tctccccacg gcattgtata tcacctcctt tcgttaatga aaatcatttt
     4501 caataagatt atacccatag agtgttcaac tgtcaagagg tgttgccgta aaaagatgtc
     4561 ttttacaggt taaatttttt tgccagagac aaagtaaaaa tctggttcgt caattatctt
     4621 caagacacta aatccatgag atgctaatgt ttttgcaagg atttttgcct caggtactct
     4681 atctttctca atgcagccac cagttgattt gtgaagatta ttgacaaaat cctttcctgt
     4741 gggatgggaa atgacaatac ttccatcttt tttcagaatt ctataaattt ctttgatggc
     4801 ggtttttttt cggtcaaaat ggggatagca cgcattacat atcacgtagt caaagtagtt
     4861 gtcaggaaaa catgtatgat gaatgtctgc aatgataaat tctacgggta aatgtctcaa
     4921 ttttcttatc gcctgttcta tcattgcagg agcataatca atggcaatca ctttaccttt
     4981 tttccctgtt ttttctagta gatatggaac aagaactccg gtgcctgtgc caacatcgca
     5041 tatcatagag ccctttttga tttctacttc ttcaacaact cttttaaggt tttttggttc
     5101 aggatgattg tcccagtgtt gtgcgtgctt ttcaaaaaaa ctacgctttc cgcatttcat
     5161 acaccatcct tttctttgga gaaaaaattg ttgcaatgat aaagattatc acagaacaga
     5221 gtgcgatggg agcgcctgca ggaatattga gccagaaggc aaccagtagt ccaagacatc
     5281 ctgatgctgc accgaatatt gaggatatga gaaacatttt ttttaaatca taggtgagtt
     5341 gataggcggc agcagcagga ttgacaataa gagcaaacac aagcagtccg ccgactgcct
     5401 ttattgaaga ggtaatggtt gctc
//
LOCUS       DAORYU010000086         5408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_10338_length_5408_cov_4.546609, whole genome shotgun sequence.
ACCESSION   DAORYU010000086 DAORYU010000000
VERSION     DAORYU010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5408
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10338_length_5408_cov_4.546609"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..596)
                     /gene="ruvX"
                     /locus_tag="PLW07_03880"
     CDS             complement(159..596)
                     /gene="ruvX"
                     /locus_tag="PLW07_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOL49696.1"
                     /translation="MENTRVLALDLGEKRIGVAISDQFRMVARGLGTIDVNGKEHEKI
                     KEIVKKYNVARIVYGVPLRDDGSLSVRAEKMLSFVTKLKNMIKAEFIPWDERMTTIEA
                     ENILLMADLSRKKRKKIRDKLAAQIILQSYLDSLYLKNTPLEE"
     gene            complement(568..>1287)
                     /gene="folP"
                     /locus_tag="PLW07_03885"
     CDS             complement(568..>1287)
                     /gene="folP"
                     /locus_tag="PLW07_03885"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOL49697.1"
                     /translation="MESEGASIIDIGGESSRPGAESLSEEEEIRRVIPVIKKLRAKTK
                     SLISCDTSRYSVAKAALEEGADIINDIYALRRDEKLIKLIADTGCGLVLMHMKGSPEN
                     MQKNPFYHDVIGEISEFFRERIDVAVENGVNIQSIIIDPGIGFGKRVIDNLEIISRLR
                     EFTCYALPILLGTSRKSFIGKISNETNPEKRLPGTIASCVAGYYGGAKIFRVHDVKDV
                     FQALKVASSIEHYGKYEGSCS"
     assembly_gap    1288..1297
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1298..1711)
                     /locus_tag="PLW07_03890"
     CDS             complement(<1298..1711)
                     /locus_tag="PLW07_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOL49698.1"
                     /translation="MEPKTRHYCVKISGVNVKQANVIKQETLAVGADAAIPYSVLNLE
                     DKIVDIVIMATVKQYRKLAEKLENQFFNLKKIGHEILKIIENYEHPSAEFLVRGRKIE
                     IKDFMIMGILNVTPDSFSDGGKFYGDVAIAVERALE"
     gene            complement(1792..2445)
                     /locus_tag="PLW07_03895"
     CDS             complement(1792..2445)
                     /locus_tag="PLW07_03895"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOL49699.1"
                     /translation="MESVRKQLCKYGKLVLEKNLAAGPGGNISARDRDFVYLSPSGFA
                     LDEIKENQWVKINLKTGKQYGSLRPTCEISMHLGCYLARPDINAVIHTHPPVTLGLIS
                     AGVELKPFYPDFVAILGREIPVIDYVIPAGEEIRQAVVEQIKKYNVVLLKNHGAVCVG
                     TTLKEAFARSWMTEETSKMQSIGMMVGKIRYFTEEEIKGIENLEAEDYRKALLKKLK"
     gene            complement(2470..3672)
                     /gene="tyrS"
                     /locus_tag="PLW07_03900"
     CDS             complement(2470..3672)
                     /gene="tyrS"
                     /locus_tag="PLW07_03900"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012991192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOL49700.1"
                     /translation="MKDIERQFEIIKKNTVDVIEEAELKKKLERSISTGKPLRVKYGI
                     DPTSPEIHIGHMVVIRKLREFQDLGHKVFFLIGDFTARIGDPTGRNETRPILSIEEIK
                     RNLTTYVEQVSKILDVNKAEFVYNGNWLRILSIEEFIKIASCFTVAQILERDDFSARY
                     KSGKAIYLHEFMYPLFQGYDSVVLASDIEIGATEQKFNLLAGRALQEFFGQEKQIVIT
                     MPILVGTDGKLKMSKTYKNHIPVSTTPDDMFGKVMSIQDNLMESYAGLLTDIDTKSFN
                     NLMKENPRQAKAYLAKNIVKQFFGEKEAEKAEENFDRIFKEKQLPEEIPVVEIEAKKF
                     GGHKANIVEILFVAGLVPSKSEAKRMIQQSAVKIDGKTISDINAEIEINGKIVKVGKR
                     KFAKIKIV"
     gene            complement(3723..4331)
                     /locus_tag="PLW07_03905"
     CDS             complement(3723..4331)
                     /locus_tag="PLW07_03905"
                     /inference="COORDINATES: protein motif:HMM:NF015423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HOL49701.1"
                     /translation="MTETYFVFLNAVGLIAFSFSGAIKGIIKELDLFGIILFGIVTAL
                     GGGAIRDIMVQKIPVMLTGPGYVFLSLVGVGAAIVVKGKRNIAVYTKIFLISDAIGLA
                     SFTATGSIIAYQSGLGMIGMIVLGMSTAIGGGIIRDILANEVPMVLNKEIYATCSFAG
                     CLVFWACYRTCDINLTSYLSMGTVFVLRILAIRYRWNLPKFS"
     gene            complement(4328..4984)
                     /locus_tag="PLW07_03910"
     CDS             complement(4328..4984)
                     /locus_tag="PLW07_03910"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOL49702.1"
                     /translation="MAIKAVIFDLDGVIFDSEILHQIAWEQIFAQYGIKLEQKDYASG
                     IGISDKDFLKKLKTEGKIPDNINIESLIEEKREKLLELSKNGAKLMPGASEFIHAMRK
                     YYVLAVASNSDIRFIRNLLEKSKLMPCFALVLGYQDIEKPKPAPDIYLKCAEKLKLLP
                     VECVVIEDSPAGIKAAKNAGMTCIAISSLLPESMLEEADFIVKTFDIERIRTSLNSIQ
                     "
BASE COUNT         1439 a         1228 c          839 g         1892 t
ORIGIN      
        1 ttcttctttt ttcaatcttt ctaccaatgt caccaggtct ttttctttat caataaaatc
       61 tgccgtgcca gcgccagtga tttgagaccc attgaaaaac gaaatgccat taagaagttt
      121 tgacattatg ctggtataat gttttattgt tttttttatc attcttccag tggtgtattc
      181 ttaaggtaca gggaatcaag gtaactctga agtataatct gtgctgcaag tttatctctg
      241 atttttttcc ttttttttct actgaggtct gccatcagca aaatattttc tgcttcaatg
      301 gttgtcattc tctcgtccca ggggataaat tctgccttaa tcatgttttt aagttttgtg
      361 acaaaggaaa gcattttttc agcgcgcaca cttaaagaac catcatctct taaaggcact
      421 ccatacacaa ttcttgccac attgtatttt tttacaatct ctttaatttt ctcatgttct
      481 tttccattaa catcaatggt tccaagtccg cgcgcaacca ttctaaactg gtcgctgatt
      541 gcaactccta tccttttttc accaaggtca agagcaagaa ccctcgtatt ttccatagtg
      601 ttcaattgat gaagccactt tcaatgcctg aaacacatct ttcacatcat gaaccctgaa
      661 aattttagcc cctccataat atcctgcaac gcaacttgca attgtcccgg gtaaccgttt
      721 ttcaggattt gtttcatttg aaattttccc gataaatgac tttcttgaag tgcccagaag
      781 tattggcaaa gcataacagg taaattccct caacctgctt atgatttcaa ggttatcgat
      841 aacccttttc ccgaacccaa ttccagggtc aatgatgatg gattgaatgt ttactccgtt
      901 ttccacagca acatctattc tctccctgaa aaattcgctt atttcgccga tgacatcatg
      961 ataaaaagga tttttctgca tattttcagg acttcccttc atatgcatca gcacaagtcc
     1021 gcaaccagtg tctgcaataa gtttaatcag tttttcatcc cgtctgagag cgtaaatatc
     1081 attgataatg tcagcgcctt cttctaatgc cgcttttgca acagaatatc ttgacgtatc
     1141 gcatgaaatc agggactttg tttttgctct cagttttttt ataacaggga tcacccgtct
     1201 tatttcttct tcttcgctca aactttctgc gcctggtctg ctggattctc cgccaatatc
     1261 aatgattgaa gcaccctcgc tttccatnnn nnnnnnnttc aagcgccctt tctacagcaa
     1321 tcgccacatc tccgtagaat ttgcctccgt ctgaaaatga atcaggagta acattcaaaa
     1381 tccccataat cataaaatct tttatttcaa tttttctacc tcttacaagg aattctgcag
     1441 atggatgttc gtaattttca attattttca aaatttcatg tccaattttc ttcaggttaa
     1501 aaaactgatt ttcgagtttt tcagcaagtt ttctgtactg ttttacagtt gccatgatca
     1561 caatgtcaac aattttgtct tcaaggttca atacagaata aggaatcgct gcgtcagcgc
     1621 caacagcaag ggtttcctgc ttgataacat ttgcctgttt tacattaaca cctgaaattt
     1681 tcacacaata atggcgggtc tttggctcca tcaagcggat gccttcaggg tgaacatcaa
     1741 tattttttaa ttctttttgt gctgtacaag gactgagaac acggatatcc attatttcag
     1801 ttttttcaga agtgctttcc tgtaatcttc tgcctcaagg ttttcaatgc ctttgatttc
     1861 ttcttctgta aagtatctga tttttccaac catcattcca atggactgca tttttgatgt
     1921 ttcttctgtc atccatgagc gggcaaacgc ttctttcaat gttgtaccca cacacactgc
     1981 accgtggttt ttcagtaaca ccacattgta tttttttatc tgttcaacaa ccgcttgtct
     2041 gatttcttca cctgctggaa ttacataatc aattactggt atttcccttc caagaattgc
     2101 gacaaaatca gggtaaaatg gtttcagttc aacacctgct gatatcaggc caagtgttac
     2161 tggtggatgg gtatgaatca cagcgttgat atcgggtcgt gcaagataac atccaagatg
     2221 catggaaatt tcacaggtgg gtctcaatga cccgtattgt tttcctgttt tcaaattgat
     2281 ttttacccac tgattttctt ttatttcatc aagggcaaag ccactcggtg aaagataaac
     2341 aaaatcgcgg tcccttgcgc tgatattccc accaggccct gctgcaagat ttttttcaag
     2401 gacaagtttt ccgtatttac acaattgctt tctcacgctt tccatttagt tgctcctttt
     2461 tctacaacct cacacaattt ttatctttgc aaacttcctt tttccaactt tcacaatttt
     2521 tccatttatt tctatttcag cgttgatatc tgaaatggtt ttgccatcaa ttttcacagc
     2581 actttgctgt atcattcttt ttgcttctga ttttgacgga acaagtcctg caacaaataa
     2641 aatctccaca atatttgctt tgtgtccacc aaattttttt gcttctatct caacaactgg
     2701 gatttcttca ggtaattgtt tttccttgaa aattctatca aagttttctt ctgctttttc
     2761 tgcctctttt tctccaaaaa actgtttcac tatgtttttc gcaagatatg cctttgcctg
     2821 ccttgggttt tctttcatca ggttattaaa actcttcgtg tcaatatctg tcaacagacc
     2881 agcgtatgac tccatcaaat tgtcctgaat ggacataact ttaccaaaca tatcatcagg
     2941 cgttgttgaa acaggaatat ggtttttata tgttttgctc atcttcaatt tgccatcagt
     3001 tccaacaaga atcggcattg tgataacaat ttgtttttcc tgtccaaaaa actcctgaag
     3061 tgctcttcct gcaagcaggt tgaacttttg ttcagtggct cctatttcaa tatctgatgc
     3121 aagtaccact gaatcatatc cctgaaaaag cggatacata aattcatgaa ggtaaatggc
     3181 ttttcctgat ttataccttg ctgaaaaatc atccctttca agaatttgag ccactgtgaa
     3241 gcaggatgct attttgatga attcttctat ggagagaatt ctaagccagt ttccattgta
     3301 aacaaattcc gctttattaa catcaagaat cttacttacc tgttcaacat aggtggttaa
     3361 atttctcttt atctcttcta ttgaaagaat cggtctggtt tcatttcttc cagtcgggtc
     3421 tccaattctt gcagtaaagt cccctatgag aaaaaacacc ttgtgcccca ggtcctgaaa
     3481 ttccctcagt ttcctgatga cgaccatatg accaatgtgt atttcagggc ttgttgggtc
     3541 tattccatac ttcaccctta atggtttacc tgttgagata cttctttcaa gttttttctt
     3601 aagttctgct tcctcaatca catcaactgt gtttttcttg ataatttcaa attgcctttc
     3661 aatgtctttc atatcatttt ctccctgatt aagcattatt attgtactcc agaatggatt
     3721 ttttaagaaa atttaggaag gttccatctg tacctgatgg caagaattct cagtacaaaa
     3781 actgttccca ttgaaagata ggaagtaaga ttaatatcgc atgttctgta gcatgcccag
     3841 aatacaagac atccagcaaa ggagcatgtc gcgtaaattt ctttattcag caccattggc
     3901 acctcattgg caagaatatc cctgataata ccaccaccga ttgctgtact cattccaaga
     3961 acaatcatac caatcattcc caggccactc tgatatgcaa tgatagaccc tgttgctgtg
     4021 aaggaagcaa gtccgatggc atctgaaata agaaagattt ttgtgtagac agcaatgttt
     4081 ctttttccct tcacaacaat tgccgcccca actccaacaa gggataaaaa cacataacct
     4141 ggaccagtca acatcacagg tattttctga accataatat ccctgatagc gccgcctcca
     4201 agcgctgtga caatcccaaa taaaataatt ccaaacaggt caagttcttt tatgatgccc
     4261 ttgattgcgc ctgaaaaaga aaacgcaata agtcccacag cattcaaaaa tacaaaatat
     4321 gtttctgtca ttggattgag ttgagagaag ttcttatcct ttcaatgtca aatgttttca
     4381 cgataaaatc tgcttcttca agcattgatt ctggaagcag ggatgaaatg gcaatacagg
     4441 tcatgccagc attttttgct gcctttattc cagcaggaga atcctctatt accacgcatt
     4501 ctactggaag cagtttcagt ttttcagcgc atttcaagta aatatccggc gctggctttg
     4561 gtttttcaat atcctggtaa ccaaggacaa gagcaaaaca tggcattaac tttgattttt
     4621 caagcaggtt tctgataaat cttatgtcag agtttgaagc cacggcaagt acataatatt
     4681 tacgcatcgc atgaatgaat tcagaagcac ctggcatcaa ttttgcgccg ttttttgata
     4741 actccagaag tttctcccgc ttttcttcaa tcaatgactc aatgttgatg ttatcaggaa
     4801 tttttccttc tgttttcaac tttttaagaa aatccttatc tgaaattcca atgccactgg
     4861 cataatcttt ttgttcaagt tttatgccat attgagcaaa aatttgttcc caggcaatct
     4921 gatgcaaaat ttcagaatca aaaatcactc catcaaggtc aaaaatcact gcctttatag
     4981 ccatcttttc ctcagtaata ggtttgtgaa aaatagagat gatgtcattt aacgatgtaa
     5041 tgatacatat tttaccacac ctgatttttt ccctgtgttt tcagggaaat tcccccgcct
     5101 ttttttctac ctcttattgt tctttgtaaa atctcttttt ttattctccc ccttttcaaa
     5161 atctccccca gcccctcttt ttcctcttct tgttttcccc ctttacaaat tcctcttctc
     5221 attctccccc ttttcaaaag ggggattaag ggggattttg aaatctcccc cagcccctct
     5281 ttttcctctt cttattctcc ccctttacaa attcctcttc tcattctccc cctttacaaa
     5341 ttcctcctcc aggtctcccc cttttcaaaa gggggattaa gggggatttt gaaatctccc
     5401 ccagcccc
//
LOCUS       DAORYU010000087         5406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_10344_length_5406_cov_4.567931, whole genome shotgun sequence.
ACCESSION   DAORYU010000087 DAORYU010000000
VERSION     DAORYU010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5406
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10344_length_5406_cov_4.567931"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..749)
                     /locus_tag="PLW07_03915"
     CDS             complement(75..749)
                     /locus_tag="PLW07_03915"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOL49703.1"
                     /translation="MSNNTIKKEVETFVDQIKKFFEEELISVVIYGSYARGDYLKDLS
                     DINILVLVKEFTSEDFVNFSEMFHKSVASTSISPVIFTPQILENSWDLFPLQWAEIKD
                     QGLVVYGRDFRHEIVVSKQSIRMQLRIDARRFYFTLQSLIVEKNYRMIFETIYRQAKI
                     IQRGLELLDIGRIEFDYLSELENLVGKKYGLFRAGKQVIKFINNHIKMLEKTISILDS
                     KDDMEY"
     gene            complement(779..1669)
                     /locus_tag="PLW07_03920"
     CDS             complement(779..1669)
                     /locus_tag="PLW07_03920"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOL49704.1"
                     /translation="MKRFSSAILSFLFLLLLSSPSYCSKQIPSPSGLLNDFAGILTSE
                     EQKNIEDRLLNIEKEKKIEMAVVILNSLEGRNIEEYANELFNQWGIGKKNQDNGVLLL
                     ISINERKIRIEVGYGLEASLTDGRCGSIIRNQIAPAFRNGKYAGGIIAGINAIEDTIA
                     GKVPADDSGKDNLAGKNSIGFLIIWQLFCLIYASMVAGFIGFIVILLEIVTFNFLFKS
                     SNNHQFFYMLALLIPFFTNFVLFFILSIFNISYTLRGKKIPKRRWLGGLPSSSGSGRT
                     SSGGGFGGGSSGGGGATGGW"
     gene            1754..2788
                     /locus_tag="PLW07_03925"
     CDS             1754..2788
                     /locus_tag="PLW07_03925"
                     /inference="COORDINATES: protein motif:HMM:NF036774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgO family outer membrane protein"
                     /protein_id="HOL49705.1"
                     /translation="MIRTRLFKSSILTCIMFHLLITASQAGIINELNSVIATISDSLS
                     INNKKIVAVTDFTDLEGNVNPLGRFIAEEISIGLTSTGKQLTIIDRNQLKKILAEKNV
                     SVSGIIDPSLAKQIGQISGAEVILTGSVYPVSGSVYLSAKIIDVSTARIFGGTSLMLA
                     KNQILDDLVQISFKTSEITVTPQQTPAFLPQVKEAANFIFILKEIQMDNNAMVLNLEI
                     KNNIDQGRYLTIKSFRAIDDSGREYFPSSFTISGAKAYIGESGKIQSIVRGTISDNFD
                     IWLRDYQQVKAQIVIRNISPKAKSIGISEMVIAPVNVTDSHGTFMRLEDDTSTIQFTN
                     LQVIRNNASK"
     gene            2775..3356
                     /locus_tag="PLW07_03930"
     CDS             2775..3356
                     /locus_tag="PLW07_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009439091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOL49706.1"
                     /translation="MPLNSDIENRYRELLEKVVIRKPELKEFVNFLENETSWLISPAS
                     TKYHLNIEHGLLIHSVNVTETMLMLKDVLMKDIPDESCVIVGLFHDVGKIGMPHAPRY
                     VKKNSGYAYNENQIEMSVAARSLYLVSKFIPLTDEEAQAILYHDGQYIMENKPVAHRE
                     CALTLLVTFADTWAAAKEDGRIKTFDQKFFKKA"
     gene            3371..3787
                     /locus_tag="PLW07_03935"
     CDS             3371..3787
                     /locus_tag="PLW07_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49707.1"
                     /translation="METTLRRYRIRTRPFLCLDPGEAADTGVTVGEETIEELTLIPNP
                     GLGFYHLYAVVINKPVSEIKDTPVMKRGEISFENRSEASHYAKKRGDPYILCGVKQTR
                     PVSKDEVEEFRNLHQQEDSIEKKLKELKMFDTRNKP"
     gene            complement(3792..4826)
                     /gene="hypE"
                     /locus_tag="PLW07_03940"
     CDS             complement(3792..4826)
                     /gene="hypE"
                     /locus_tag="PLW07_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007219965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HOL49708.1"
                     /translation="MPHTTPMKDDKIFLSHGAGGKQSRTLVEKIILKYFNDPLLVALP
                     DSATFDILDSRKTFCFTTDSYVVNPVFFPGGDIGKLAVCGTVNDLAVCGAKPLFISCA
                     MIIEEGFSINDFERILRSMATTARKVGVRIVTGDTKVVDRGKCDGIFITTSGIGIKKN
                     NVCSGLSSIRPGDAIILSGVPGLHELSIICAREHFNFTVSLKSDCAPIYFLVEKMLGS
                     SARIKFMRDPTRGGVASVLNEIVAGNNFGIRIYEEKIPLTEQVRSLCDIFGFEILHLA
                     SEGRVVMVVDKNDAQKLLDVMKKHPLGKRAEIIGNVIREFPGRVILKTKFGSERFVEM
                     PSGKQLPRIC"
     gene            complement(4795..>5406)
                     /gene="hypD"
                     /locus_tag="PLW07_03945"
     CDS             complement(4795..>5406)
                     /gene="hypD"
                     /locus_tag="PLW07_03945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HOL49709.1"
                     /translation="NNFMVLAGNKLIPPAMEMLVRGTRHSIDGFICPGHVSIVIGAKP
                     YEIFPKNYGIPCVISGFEPCDILQSILLLLEMISGKQRNEVKIQYSRCVAKKGNLTAR
                     KIMNEAFDVVDSEWRGIGIIPSSGLSIKEQFKEYDAECVLNLDVKISRDKKGCLCGDV
                     LKGLKTPQDCVFFAKKCTPQHPVGPCMVSSEGTCAAYYAYERR"
BASE COUNT         1772 a         1131 c          905 g         1598 t
ORIGIN      
        1 aaagaaaaat gccttataga aatataattt caaatagatt tatttttgag gaaatgtgct
       61 tcttcacatt attttcagta ttccatatcg tcttttgaat caagaatcga aattgtcttt
      121 tccagcattt tgatatgatt gttgataaat ttgataacct gttttcctgc tctaaaaagt
      181 ccgtattttt ttcctaccag attctccagt tcactgagat aatcaaattc aattctgccg
      241 atatcaagaa gttccaatcc cctttgaata atctttgcct gtctgtatat tgtttcaaag
      301 atcatcctgt aatttttttc cactatcaaa ctttgcaatg taaaataaaa gcgtcttgca
      361 tcaattctca gttgcatccg tattgattgt ttcgaaacca caatttcgtg gcgaaaatcc
      421 cttccataaa caacaagtcc ctggtccttt atttctgccc attgcaaagg aaaaaggtcc
      481 catgagtttt ccaaaatttg aggagtaaaa atcacagggc tgattgaagt tgaagcaaca
      541 gatttatgaa acatctctga aaaattgaca aaatcttctg atgtaaattc cttcacaagc
      601 acaaggatat tgatgtcaga caaatccttc aaataatcac cccgagcata acttccatag
      661 attacaacac tgatcaattc ttcttcaaaa aatttcttta tctgatcaac aaatgtttct
      721 acttcctttt ttattgtgtt gttagacaca attacctcct gtgattttaa taaaacaact
      781 accatccgcc tgttgcccca ccgccaccac ttgaaccacc tccaaatccg cctcctgaag
      841 aagtccttcc actaccagaa gacgatggca atcctcccag ccagcgcctt tttggaattt
      901 tcttgcctct caatgtataa gaaatattaa aaattgataa aataaaaaac agaacaaagt
      961 tggtgaaaaa cggaatgaga agcgcaagca tataaaaaaa ttggtgattg ttgctggact
     1021 taaacagaaa gttaaaagtt acaatttcaa gaagaataac aataaatcca ataaaacctg
     1081 cgaccattga agcataaatc aagcagaata gttgccagat aatcaaaaaa ccaatagaat
     1141 tcttacccgc aagattgtcc ttgcctgaat catcagcagg cactttaccc gcgatagtgt
     1201 cctcaatggc atttattcct gctattattc cacctgcata tttaccattt ctgaatgccg
     1261 gagcaatttg atttctgatt attgaaccac atcttccatc tgtcagcgat gcttcaagac
     1321 catatccaac ttcaatcctt atttttcgtt cgttgatgga aataaggaga agaacaccat
     1381 tgtcttgatt ttttttgcct ataccccact ggttaaaaag ttcattcgcg tattcttcaa
     1441 tgtttcttcc ttcaagtgaa ttgagaataa caacagccat ttcaatcttt ttttcttttt
     1501 caatgttcag caatctatct tctatatttt tttgttcttc tgaggtcaaa atccctgcaa
     1561 aatcgttcag taaacctgaa ggagatggga tttgttttga gcaatatgat ggagaagata
     1621 agagcaaaag aaataggaat gatagaattg cagatgagaa tcttttcatc gtatttcctt
     1681 ttcaaataaa tattgacttc ttcaaactcc ttaaatatga taccatatat acgaattaat
     1741 aacaggatac attatgatca gaacacgcct ttttaaatct tccatcttga cctgtattat
     1801 gtttcactta ttaatcactg cttcacaagc cgggatcatc aatgaactaa actctgttat
     1861 tgccacaatt tctgactccc tttcaataaa taacaaaaaa attgtcgcgg tcactgattt
     1921 tacagacctc gaaggtaatg taaatccact cggaagattt atcgctgaag aaatttcgat
     1981 tggtcttaca tcaacaggta aacaattaac aatcattgat cgtaatcaac taaaaaaaat
     2041 ccttgctgaa aaaaacgttt ctgtttctgg catcattgac ccttcccttg ctaaacaaat
     2101 cggacagata tcaggcgctg aagtaatcct gaccggcagt gtgtatccag tttcaggatc
     2161 agtgtatctt tcagctaaaa ttattgatgt aagcactgcg agaatttttg gcggtacatc
     2221 tctaatgctc gcaaaaaatc agattcttga cgacctggta caaatcagtt ttaaaacgtc
     2281 tgaaattaca gtaacccctc agcaaacccc tgctttttta cctcaggtaa aagaagccgc
     2341 taattttata tttatactca aagaaattca aatggataac aatgcgatgg ttctgaattt
     2401 ggaaataaag aataatattg accagggccg ttacctgact atcaaatcat ttagagcaat
     2461 agacgactct ggcagagaat attttccgtc atctttcaca atttcagggg caaaagcata
     2521 cataggtgaa tccggaaaaa ttcaatcaat agtcagagga acaatatctg ataattttga
     2581 tatctggcta cgcgactatc aacaggtaaa agcgcaaatc gtcataagaa acatttcgcc
     2641 aaaagccaaa tccataggca tttcggaaat ggtgattgct cctgtgaatg ttacggacag
     2701 tcatggaacc ttcatgcgac ttgaagatga tacttcaaca attcagttca caaatctcca
     2761 ggtcataaga aacaatgcct ctaaatagtg atatcgaaaa taggtataga gagttgctgg
     2821 aaaaggtcgt aattagaaaa ccagaactca aggagtttgt aaattttctt gaaaacgaaa
     2881 catcctggct catatcacct gcgagtacaa aatatcatct caacattgaa cacggacttc
     2941 ttatccattc tgtgaatgta acagaaacaa tgctgatgtt gaaggacgtt ctgatgaaag
     3001 acattccgga tgaaagttgt gtgattgtgg gactttttca tgatgtggga aaaattggca
     3061 tgccccatgc tccaagatat gtgaaaaaaa attcaggtta tgcgtacaat gaaaaccaga
     3121 tagaaatgtc tgttgccgca agaagtcttt atcttgtttc aaagtttatc ccgcttactg
     3181 atgaagaagc acaagcgatt ctttatcatg acggtcaata cattatggaa aacaaacctg
     3241 ttgctcaccg cgaatgcgcc ctgacactgc ttgtcacttt tgctgataca tgggctgcgg
     3301 cgaaagaaga cggcagaata aaaacgtttg atcaaaagtt tttcaaaaaa gcataaacag
     3361 ggggtaaaaa atggaaacta ctctcaggag atacagaata agaacgaggc cattcttgtg
     3421 tcttgacccg ggagaggctg ctgacacagg cgtaacggtt ggagaagaaa ccattgaaga
     3481 actgacactt attccaaatc caggactggg attctatcac ctgtacgctg tagtaattaa
     3541 caaacctgtc agtgaaatca aagatacccc ggtaatgaaa aggggagaaa tctcttttga
     3601 aaacagatca gaagcatcac attatgcaaa aaagaggggc gatccttaca tcctatgcgg
     3661 tgtgaagcag acaagaccgg taagcaaaga tgaggtggaa gaatttcgca accttcatca
     3721 gcaggaagat tccatagaaa agaagttgaa ggaactgaaa atgtttgaca ccaggaacaa
     3781 gccataaaat tttaacaaat ccttggtaat tgctttcctg aaggcatttc aacaaatctt
     3841 tcggaaccaa attttgtttt caaaatcacc ctgcctggaa attctctgat aacatttcct
     3901 atgatttctg cccttttacc aaggggatgt tttttcatca catcaagcaa tttttgtgca
     3961 tcatttttat caaccaccat aaccaccctg ccctcgcttg caagatgcaa tatctcaaac
     4021 ccaaagatat cacaaaggga acgtacctgc tctgtcaaag gaattttctc ctcatagatt
     4081 ctaataccaa aattatttcc cgccacaatt tcatttaata cagaagcaac gcctcccctt
     4141 gttggatctc tcatgaactt gattcttgct gaacttccca acattttttc aacaagaaaa
     4201 taaatgggag cacaatcact tttcaatgat actgtaaaat taaaatgttc tcttgcgcat
     4261 ataattgata actcatgtaa accaggcaca ccgctgagaa taatcgcatc gcctggtcta
     4321 atgctgctca agcccgaaca aacattattt ttcttgattc caatacctga tgttgtaata
     4381 aaaattccat cgcatttacc tctatcaaca acctttgtat cacctgtcac aattcttact
     4441 ccaactttcc tggcagtcgt tgccatggac ctgagtatgc gttcaaaatc attgatactg
     4501 aaaccctctt caataatcat tgcgcatgaa ataaatagcg gtttagcgcc acaaacagca
     4561 aggtcattaa cggttccaca cacagcaagt tttccaatgt ctccaccagg gaagaatact
     4621 ggattcacaa cgtaggagtc tgttgtaaaa caaaaagttt ttcgtgaatc caaaatgtca
     4681 aaggtcgctg aatcaggcag tgccaccagc aaagggtcat taaaatactt caggataatt
     4741 ttttcaacca gtgtccttga ttgctttcca cctgctccgt gcgaaagaaa tattttatcg
     4801 tctttcatag gcgtagtatg cggcacacgt tccctcagaa gacaccatac acggtcccac
     4861 aggatgctgg ggtgtacact tttttgcaaa aaaaacacag tcctgaggtg ttttcagtcc
     4921 tttcagtaca tctccgcaga gacaaccctt tttatcacga gaaattttaa catctaaatt
     4981 cagaacgcac tcagcatcat attctttgaa ttgctctttt attgaaagtc cgcttgatgg
     5041 aattattcca attcctctcc attcagaatc aaccacatca aaagcttcgt tcataatctt
     5101 ccgggcagtc agatttcctt ttttcgcaac acagcgagaa tactgtattt tcacttcatt
     5161 gcgctgtttg cccgatatca tctcaagtag taacagtatt gattgaagaa tatcacatgg
     5221 ttcgaaacct gaaataacac atggaatacc ataatttttg ggaaaaattt cataaggttt
     5281 tgcgcctatg acaatactga catgccccgg gcagataaat ccatcaatag aatgtcgagt
     5341 acccctgaca agcatttcca tcgcaggagg aattaattta ttgcctgcca gtaccataaa
     5401 gttatt
//
LOCUS       DAORYU010000088         5391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_10378_length_5391_cov_4.414168, whole genome shotgun sequence.
ACCESSION   DAORYU010000088 DAORYU010000000
VERSION     DAORYU010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5391
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10378_length_5391_cov_4.414168"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..363
                     /gene="secA"
                     /locus_tag="PLW07_03950"
                     /pseudo
     CDS             1..363
                     /gene="secA"
                     /locus_tag="PLW07_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005775809.1"
                     /note="functions in protein export; can interact with
                     acidic membrane phospholipids and the SecYEG protein
                     complex; binds to preproteins; binds to ATP and undergoes
                     a conformational change to promote membrane insertion of
                     SecA/bound preprotein; ATP hydrolysis appears to drive
                     release of the preprotein from SecA and deinsertion of
                     SecA from the membrane; additional proteins SecD/F/YajC
                     aid SecA recycling; exists in an equilibrium between
                     monomers and dimers; may possibly form higher order
                     oligomers; in some organisms, there are paralogous
                     proteins that have been found to be nonessential but do
                     function in secretion of a subset of exported proteins;
                     frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
     gene            667..1599
                     /locus_tag="PLW07_03955"
     CDS             667..1599
                     /locus_tag="PLW07_03955"
                     /inference="COORDINATES: protein motif:HMM:NF019155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HOL49710.1"
                     /translation="MGQLPDGEKIKHPLITRMINNAQRKVEARNFEIRKQLLEFDDVM
                     NEQRKVIYGIRQDILSGRNIDKYLEDFINETIDLAIDEHLNLQVKPDNWNVEALSTHL
                     KNIFNISVNLVIPETSRDIMAWRDDIGEEIKNSVKHALEQQVQAMGDIFEEVLRLVFL
                     QAIDHRWKSHLRSIDELREGIGLRAYGQRDIIVAYKQEAFLLFEEMLGSIRQEILTHI
                     FRVKVSEGAVAKAPSRVFVPAQYAHQEMNQFDAVKLAEPAQIGQQLSSSPGDIPNQPA
                     QKPAPVRLGKKVGRNDPCPCGSGKKYKKCCGANQ"
     gene            1596..2930
                     /gene="dnaA"
                     /locus_tag="PLW07_03960"
     CDS             1596..2930
                     /gene="dnaA"
                     /locus_tag="PLW07_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOL49711.1"
                     /translation="MNEYSVDKLWKIWESVLNRMKEAVQNDRVIETWFKPLKINKIDN
                     NIIYMEIPNQLFYKGLAPFLDRFKEILAETLGFAPELHWILPEGSSSYRLEGTETREQ
                     MIGENLNPDYIFETFVVGPCNRLAHAGSLAVAQSPGIAYNPLFIYGDVGLGKTHLMQA
                     IGNFVGRANQQTNIAYMPCEIFVNQFIYSIQTKTAHMFRNKFRKVGVLLIDDIHFLAG
                     KEGTQEEFFHTFNALYDQRKQIVLSSDRPPKEIADLEKRLVSRFEWGLVVDLQPPDFE
                     TRVAILKKKCETKNLVLSDDVIFYLAENISGNIRLLEGALNRLVALSSLFDQKLNIDV
                     AKEYLKEMLHSQKNLITIQRIQEVVAEYFRISIHDLKSQRRLKNLVIPRQIAMHLARE
                     MTASSLTAIAEEFGGKDHTTVIHACKRIKSAIEEDTNVRSIVNRIINILNAK"
     gene            3023..4123
                     /gene="dnaN"
                     /locus_tag="PLW07_03965"
     CDS             3023..4123
                     /gene="dnaN"
                     /locus_tag="PLW07_03965"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOL49712.1"
                     /translation="MEIVIEKGKLLDGLDKISKILPTKPVIATTGGILVDGKKDGITL
                     TATDLQLTIKIFIEAAVKDKGLILIPGKNFISLVKKIPEESIKITTRENSILVENKSF
                     QYKFLLMDAEEYPKLPFDKEQTSESGDLVISSSILLDMIKKTSYCINPDEPRMYFRGV
                     LMEKKGNLLNMVATDTRRLSLIRKEIQGEGKVKIILPMRLIDVFPVIFKEADVSMSFS
                     KNQITLKSDRTTLISQLLEGEFPDYEKVIPAANKTGTAIIKTEQLLSSLERLSLMSSE
                     SVSSVKLNFRKNLLVLNIESPESGSGEEKLNIEYAGAENTIVFNPEYIIQFLKTVTQE
                     EIEFFFQDPVKPARLCGTGDQEYIYVAMPMRQ"
     gene            4120..4419
                     /locus_tag="PLW07_03970"
     CDS             4120..4419
                     /locus_tag="PLW07_03970"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HOL49713.1"
                     /translation="MKETKLADIVGDVLKNILKNTGKPEQVRHEGIIDWDKIWAEVCE
                     KARPYSYVVTMFEKTLVVRVKNSAWVLELRKNEKEIINKLQARTGRKLEKIMFVR"
     gene            complement(4421..>5391)
                     /locus_tag="PLW07_03975"
     CDS             complement(4421..>5391)
                     /locus_tag="PLW07_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49714.1"
                     /translation="NYAFGFERMHTWLALAHSQIPVDFLSEKMIEEGWLSKYKVAYFS
                     GTAITEKAAEQMKLWVLKGGVLVLTAGAGMKDQFNRPLKSLDSILPAERSSIIEIARY
                     LNSGRYLDSLSVADTVIVKETGAVFDVLAVKQTLKPKPGSVISGTFSDSSPSIVSGKA
                     GRGTILCCGFLPAIAYMRKALIARNQLMKDAGDLEKVQEPLPSEVATDIQLIKRAFEP
                     WQYPEEYRNLISMPMYQTECALPVKCSTALVDAIVMDTEKGSVVVLSNYTFQPITELT
                     LDVKPLRRVTKVESVRCGSIRFTQHSDHIRFSLPLRETDFVKIYYR"
BASE COUNT         1831 a          989 c         1147 g         1424 t
ORIGIN      
        1 cctgacagaa gcagttgaac tcaaaacgat ttataaggtg gatgtgcttg tgataccgac
       61 caacaaacca ctcaatagaa gagaattcga tgatgagatt tacaaaacag aaaaagaaaa
      121 atttgaatct gtcgctgcag aaatagaaca gctgtacaaa actggcaggc ctgttcttgt
      181 tggaaccact tccattaaaa atcagaaagg ataagtagaa tactgctgag aaaaaatatc
      241 ccacatcagg tattgaacgc aaaatatcac gaaaaagaag cagaaatcat tgccaaagca
      301 ggtcagaaat ttgctgtcac cattgcaaca aatatggcag gaaggggaac agacatcgtc
      361 ctgggcgaag gcgttgtaca taaagaatgt ataaaccctg aaacaggcca gagtaagtgt
      421 tgtattgcgt gtgaagatga ctgtgcaacc tgtttcaaaa aaaggaaatt ttcagaatgc
      481 agaaccagac aggatattga ttgcggcttg catgtggtgg ggacagaacg acatgaagca
      541 agacgcattg acaaccagct caaaggtagg tctggcagac agggagaccc tggttcttca
      601 cgattttacc tttcccttga agatgacctg ttgagaattt ttggttcaga tagattgaaa
      661 ggaatgatgg gacaacttcc tgatggagaa aaaataaaac atcctctgat tacaagaatg
      721 attaacaatg cccagagaaa ggtagaagcg agaaactttg agatacgcaa gcaacttctt
      781 gagtttgacg atgtgatgaa cgaacagaga aaggtcatct atggaatacg gcaggacatt
      841 ctttctggca gaaatattga taaatatctt gaggatttta tcaacgaaac aatagacctg
      901 gctatagacg agcacttgaa tttacaggta aaacccgaca actggaatgt tgaagccctc
      961 tcaacccatc tcaagaacat ttttaacatt tctgttaacc tggtcatccc tgaaacatca
     1021 agagatatca tggcatggag agacgacatc ggcgaagaaa ttaaaaatag tgtaaagcat
     1081 gccctggagc aacaggtaca ggcaatgggc gacatatttg aagaagtgct ccggcttgtc
     1141 ttccttcagg caatagacca cagatggaaa tctcaccttc gttccataga tgaactgaga
     1201 gagggtatag gtttaagagc atatggccag cgtgatatta ttgttgccta taaacaggaa
     1261 gcatttttat tatttgagga aatgctgggt tctataaggc aggaaattct cacccatata
     1321 ttcagggtga aagtgtcaga aggtgcggtt gcaaaagcac cttcacgggt atttgttcca
     1381 gcccagtacg cccaccagga aatgaaccaa tttgatgctg tgaaactggc agaacctgca
     1441 cagataggtc agcaattatc ttcatcccct ggtgacattc ccaatcaacc agcacaaaaa
     1501 cctgcacctg tcaggctggg taagaaagta ggaagaaacg atccctgtcc atgtggaagc
     1561 ggaaaaaaat acaaaaaatg ctgtggagca aaccaatgaa cgaatattct gtggataagt
     1621 tgtggaaaat ctgggaaagc gtgttaaacc gaatgaagga ggctgttcag aatgaccggg
     1681 tcatagaaac atggtttaag ccattgaaaa tcaataagat agataacaat ataatataca
     1741 tggagattcc caatcaactt ttctacaaag gtcttgcacc gtttctggac agatttaagg
     1801 aaattcttgc tgaaaccctt ggttttgccc ctgaattgca ctggattttg cccgaagggt
     1861 cttcgtcata tagattagaa ggcactgaaa ccagagaaca gatgatcggc gagaacctaa
     1921 accctgacta tatctttgaa acttttgttg ttggtccatg caacagatta gcacatgctg
     1981 gcagtcttgc tgttgcgcag tctcctggga tagcatacaa tccgcttttc atttatggag
     2041 atgttgggct tggaaaaacc catctcatgc aggcaattgg aaattttgtt ggcagggcta
     2101 atcagcaaac aaatattgct tatatgccct gtgaaatatt tgtgaatcag tttatttata
     2161 gtattcagac caaaaccgcg catatgttca gaaataaatt cagaaaggta ggtgttctgt
     2221 tgatagacga tattcacttt cttgcaggaa aagaaggtac gcaggaagag tttttccaca
     2281 ccttcaatgc cctttacgac cagagaaaac agattgtgct ttccagcgat agacctccca
     2341 aagaaattgc agaccttgaa aagcggctgg tttccagatt tgagtggggt ctggttgtgg
     2401 acctgcaacc gcctgacttt gaaacaaggg ttgccatact gaagaaaaaa tgcgagacaa
     2461 agaatcttgt tttaagtgat gatgttattt tttatcttgc agaaaatatc agtggcaata
     2521 taagattgct tgaaggtgct ctgaataggt tggtagccct ttcgtcttta tttgaccaga
     2581 aacttaatat tgatgttgca aaagagtacc tgaaggaaat gttacattcc cagaaaaacc
     2641 ttattacgat tcaaagaatt caggaggttg tcgcagaata tttcagaatc agtattcatg
     2701 accttaaaag tcagcggcgg ttgaaaaatc ttgttattcc aagacagatt gcgatgcatc
     2761 ttgcgcgtga aatgacagca agctcgctta ctgccattgc agaggaattt ggaggcaaag
     2821 accatacaac agtaatacac gcgtgcaaaa gaattaagtc agctatagaa gaagatacaa
     2881 acgtcaggtc tattgtcaat agaattatta acatattaaa cgcaaagtaa ttcacatgtt
     2941 attaacactg caaataacgt tttttgtaga tacagattga aattaacata gattactggt
     3001 tttattactg aaaggagata atatggaaat agtaatagaa aaaggaaaac tgcttgacgg
     3061 ccttgataaa atttcaaaaa ttcttccaac aaaaccggtt attgcaacaa ctggtggaat
     3121 tttggtagat ggaaaaaaag acggtattac tcttacagca accgatttac aattaacaat
     3181 aaaaattttc attgaagcag ccgtaaaaga caagggactc atattaatac caggaaaaaa
     3241 ttttatttcc cttgtaaaaa aaatccctga agaaagcatt aagattacaa ccagagaaaa
     3301 cagtatattg gtagaaaaca aatcgtttca gtataaattt ttactcatgg acgctgaaga
     3361 gtatccaaaa cttccgtttg ataaagaaca aacatctgaa tcaggagatc ttgtaatatc
     3421 ttcatcaatt cttcttgata tgatcaaaaa aacaagttac tgtatcaatc ctgatgaacc
     3481 gagaatgtac ttcaggggcg tgttgatgga gaaaaaaggc aaccttctta atatggttgc
     3541 aaccgacaca cgaagactgt cccttataag aaaagaaata cagggcgaag gaaaggtgaa
     3601 aattatactt ccaatgagat taattgatgt atttcctgta attttcaaag aagcagacgt
     3661 ttcaatgagt ttttcaaaaa accagattac attgaaatct gatagaacga cattgatatc
     3721 ccagcttctg gaaggagagt ttcctgatta cgaaaaagtt attcctgcgg ctaacaaaac
     3781 aggtaccgct attatcaaaa ccgaacagtt gctcagtagt ttggaacgac tttctctgat
     3841 gtcgtcagaa agtgtgagtt ctgtgaagtt gaatttcagg aaaaatctcc ttgtattgaa
     3901 catcgaaagt cctgaaagcg ggtcaggtga ggaaaaactg aatatagagt acgcgggagc
     3961 ggaaaataca atcgtattta acccggaata catcatacag tttctcaaga cagtaacaca
     4021 ggaagaaata gagtttttct ttcaggatcc ggtgaaaccg gcaaggttat gcggaacagg
     4081 agaccaggaa tatatttatg tcgcaatgcc catgagacaa tgaaagaaac aaaactggcg
     4141 gacattgtgg gtgatgttct gaaaaatatc ctgaaaaaca caggtaaacc tgaacaagtc
     4201 cgccatgaag gaattataga ctgggacaaa atctgggcag aggtttgcga aaaagcaaga
     4261 ccttatagtt atgtggtgac gatgtttgaa aaaacactgg ttgtcagggt aaaaaactct
     4321 gcatgggttc tggaactaag gaaaaacgaa aaagagatta tcaataaact tcaagccaga
     4381 acaggcagga aattagaaaa gatcatgttt gtcagatagg ttatctgtaa tagattttca
     4441 caaaatcagt ttcccgaagg ggcaacgaaa atcgtatatg atccgaatgc tgagtaaatc
     4501 taatagagcc acagcgaaca ctttcaactt tcgttacacg tcgtaatggt ttcacatcaa
     4561 gcgttagttc tgttattggt tgaaaagtgt aatttgaaag gacaaccacg cttccctttt
     4621 ctgtatccat cacaattgca tcaacaagcg cagtagaaca tttaacaggt agagcacatt
     4681 ctgtctgata cattggcatg ctgattaagt ttctgtattc ttcaggatac tgccatggtt
     4741 caaaagctct tttaatcaac tggatgtctg ttgcaacctc tgaaggcaga ggttcctgca
     4801 ccttttcaag atcaccagcg tccttcatta actgatttct tgctattagt gcctttctca
     4861 tgtaagcaat agcaggaaga aacccacagc ataagatcgt acctctgcct gcctttcctg
     4921 atactatgga ggggcttgag tcagaaaatg tacccgaaat cactgaacca ggttttggtt
     4981 tcaatgtttg tttgacagca agaacgtcaa aaaccgcgcc agtttctttt acgatcacag
     5041 tatcagccac agaaagagag tcaaggtatc taccactatt aagatatcgt gcaatttcaa
     5101 ttatggaaga tcgttcagcg ggcagtattg aatcaagact cttaaggggt ctattaaatt
     5161 gatccttcat accagcgcca gcagtaagca caagcacgcc accttttaat acccataatt
     5221 tcatttgttc tgcagctttt tcagttattg ctgtgccaga aaaatatgcc accttgtatt
     5281 tagaaagcca gccctcttct atcatttttt ctgatagaaa atctactggt atctgactgt
     5341 gggcaagggc aagccatgta tgcatcctct caaagccgaa tgcgtagtta t
//
LOCUS       DAORYU010000089         5370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_10435_length_5370_cov_3.627658, whole genome shotgun sequence.
ACCESSION   DAORYU010000089 DAORYU010000000
VERSION     DAORYU010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5370
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10435_length_5370_cov_3.627658"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..1159)
                     /locus_tag="PLW07_03980"
     CDS             complement(17..1159)
                     /locus_tag="PLW07_03980"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018400558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOL49715.1"
                     /translation="MNVSILDLKREYQYMKNDILNAINKCLEHQSWILGPEVKELEQK
                     IAEFLDIKYCVGCSSGTDALVLGLRAIAIEKGMEFFSPHDEIITTPFTFTATGDAILR
                     SGATPVFVDIEPDTFNINPEFITTYLQSEHGKNVKGIIPVHLYGTPCNMDAIMNIAHQ
                     YEIFVLEDVAQAFGGSYDNSKLGTIGDIGAFSFFPSKNLGGYGDGGMVACHNASTENI
                     IRTLIKHGGKDKYNVDHIGYNARLDTIQAAILLVKLIYLDEFNERRKKIAQRYNQELS
                     EIKEVKVPISIQESVFHQYTIRVKNGKRDQLQAFLKEKCIGTMVYYPFCLHQMKLFQG
                     RAKVQGKMEQAEKATQEVLSLPIEPLMTEEEQTYVIDTIKEFFTKS"
     gene            complement(1168..1746)
                     /locus_tag="PLW07_03985"
     CDS             complement(1168..1746)
                     /locus_tag="PLW07_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HOL49716.1"
                     /translation="MKDYFIHPSSFIDDNVVIGKGTKIWHFCHILSNTKIGDNCILGQ
                     NVMAGPDVIIGNNCKIQNNVSLYKGIYLEDDVFCGPSCVFTNVINPRAFVERKHEFKN
                     TLVKKGATIGANATIVCGNTIGKYALIGAGAVVTHDVCDYALVVGVPAKQIGWVCKCG
                     VTLSRGNEELKCKACGNIYIVVNNQLEPVLEK"
     gene            complement(1743..2747)
                     /locus_tag="PLW07_03990"
     CDS             complement(1743..2747)
                     /locus_tag="PLW07_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOL49717.1"
                     /translation="MDKNCQDKSITNKAQIGVIGGGYWGKNLIRVFYQLGVLKFICDS
                     NEELMANYRNNYPDVFFTTQPEELIKKNDIDAIVIATPASTHYSIAKISLEHNKHVFC
                     EKPLALTVKEGEDLSRIAEEKNLILMVGHILHYHPAIKKLKQIIRNKELGDIEYMYSN
                     RTNIGRIRHEENILWSFAPHDISLILSLAGCLPARVIAHGGIFLPHDVEDVTLTFLSF
                     PNNVKAHIFVSWLNPFKEQKFVVIGTKKMAVFDDTERDNKLVLYQHKISWKENDIPLI
                     EKAEAEIVETEKTEPLKEEAEHFIHCIETKEKPLTDAQEALNVLKVLEMAEHSLKESR
                     "
     gene            complement(2717..3457)
                     /locus_tag="PLW07_03995"
     CDS             complement(2717..3457)
                     /locus_tag="PLW07_03995"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOL49718.1"
                     /translation="MKNISGIILAGGKSSRYGQAQYKFLVEYQGKPLITKIIGAIQNV
                     VGKIVIVISPHACQTKQILSNFNNIEYVIQDRPLGTGHALLSTHSIFENTDRTLLVTY
                     ADKPLVTTATFKKLVDQHIQNNADITIATTILPNPGSKGRVIRIDGRFAGVIEAKDAD
                     ETTLQIKEVNAGFIVCESRSIYNELRKIKNNNAAGEYYLTDVYAEYIEDGLKVQTVEI
                     PAEESCDINTIEEFKRIEEWIKTVRTKV"
     gene            complement(3460..4449)
                     /locus_tag="PLW07_04000"
     CDS             complement(3460..4449)
                     /locus_tag="PLW07_04000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOL49719.1"
                     /translation="MKTGAFGDMALFSISINAVSMAFPDAQLYLLTSSSYAEMYKDCP
                     MIKKVFTLPASRNPLPFINIVRTLRKKKFDVIFDLQGNLKTNFLAFLLGSKQRVGIYK
                     KPIGKLFLTEGIKKLHNVNAIESQVQFWKKFTNKPVGKKLQIWISDEKRKNLKNYLDV
                     HKLVINRYIVFHTSASHKWKTKLWPINHWINLGMSLAKKGFDIVLVGDKNAMRINTVI
                     ANKIGTNVIDLSGKTDFFTLAFVIENAALLITTDSGPMHVGAATGTRTVAIFGPTDPR
                     YHCPSGITFIQANNVCPPCYKKQCEHHTCMKSISPRYILNVIKVENEQIHCII"
     gene            complement(4475..>5370)
                     /locus_tag="PLW07_04005"
     CDS             complement(4475..>5370)
                     /locus_tag="PLW07_04005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOL49720.1"
                     /translation="KTPVIFWKSLKVLQKLIADEKPDILHCRSRFPAWIGYYALSSKQ
                     QKTKFVTSIHGFYGSSFYSKIMGKGERVIVISDTLGEFAIKKLGADPHRVRVVHNGIR
                     FEPYINLSYKKHSGFIIGCIGRLTKLKGFQYLIQAVAIAKQNVPNIKIHIIGDGPYKK
                     NLEYLGKKLGVTTLFLKGRASDFLPEIDLLVAPHIETETLKKNIIPWLGRSVYEAQLA
                     GIPVLTTLNGIEKGTFIKTKTELLTAPCDIEAMAKAIIFVIQNPEESKHIATNGKEFV
                     MNHFSIQQMVEKTVQVYQELL"
BASE COUNT         1465 a         1017 c          928 g         1960 t
ORIGIN      
        1 tctctttttt agaattttaa gattttgtaa agaattcttt aatggtgtct attacatacg
       61 tttgttcttc ttctgtcatc aatggttcaa tgggtaaact cagtacttct tgggttgctt
      121 tttctgcttg ctccatttta ccctgcacct tcgccctacc ctgaaaaagt ttcatctgat
      181 gcaaacaaaa tggatagtag accattgttc caatacattt ttctttgaga aatgcctgaa
      241 gttggtcccg ttttccattt tttacccgga ttgtatactg atgaaaaacg ctttcttgaa
      301 tagagatagg aacttttact tctttaattt cagaaagttc ttgattatat cgttgtgcga
      361 ttttttttcg cctttcattg aattcatcta agtatatgag tttaacaaga agaattgctg
      421 cttgaatggt atcaagtctc gcattataac caatatggtc aacattatat ttgtctttcc
      481 ctccatgttt tattaaagtt ctaataatat tttctgtgga tgcattatga catgcgacca
      541 tacctccatc tccataacca ccgagatttt tgctcgggaa aaaactgaaa gctccgatat
      601 cgccaattgt gccaagtttg ctgttatcgt aacttccacc gaatgcctgg gctacatctt
      661 ctaaaacgaa aatttcatat tgatgtgcta tattcataat tgcatccata ttacatggtg
      721 tgccgtaaag gtgcacagga ataataccct tgacgttttt cccgtgttct gactgcaaat
      781 atgttgtaat aaactctggg ttgatattaa aagtatccgg ctcaatatct acaaatactg
      841 gcgtggcgcc acttcgtaaa atggcatcac cagttgcagt aaatgtgaaa ggtgtagtaa
      901 tgatttcgtc atgtggagaa aaaaattcca tacccttttc aatcgcaatt gctctcagtc
      961 caagcacaag ggcatctgtt ccagatgagc accccacaca atattttata tcaaggaatt
     1021 cagcaatttt ttgctcaagt tctttaactt ctggaccaag aatccatgac tggtgttcaa
     1081 gacatttgtt gatggcattg agaatatcat ttttcatata ctggtattct cttttaagat
     1141 caagtataga aacattcata tttgctccta tttttctaag acaggttcta attgattatt
     1201 gacaacgata taaatatttc cgcacgcctt gcatttgagt tcttcatttc cacgggaaag
     1261 tgttactcca catttgcata cccaccctat ctgttttgcg ggtacgccaa cgaccagtgc
     1321 ataatcacat acatcgtgcg tcacaaccgc acctgctcca ataagtgcgt atttgccaat
     1381 tgtatttcca catacaattg tcgcgtttgc ccctatagtg gctccttttt ttaccagggt
     1441 gtttttgaat tcatgttttc tttccacaaa tgcccttgga ttgataacat ttgtgaaaac
     1501 acatgatgga ccgcagaaaa catcgtcttc aagatatatt cccttgtaaa gcgaaacatt
     1561 gttttgaatt ttgcaattat tcccgataat aacatcgggt cctgccatta cgttttgtcc
     1621 caggatacaa ttatcgccta tttttgtatt ggaaagtata tgacaaaaat gccatatttt
     1681 agtacccttg cctatcacaa cgttatcatc aataaatgaa gatggatgta tgaagtaatc
     1741 tttcatcttg actcctttaa tgaatgttca gccatttcga ggactttcag tacattcaat
     1801 gcttcctgag catctgtaag tggtttttcc tttgtttcga tacaatggat gaaatgttct
     1861 gcctcctctt tcaagggttc tgttttttct gtttcaacaa tttctgcttc agccttttct
     1921 attaatggaa tatcgttttc tttccacgag attttatgtt gataaagtac aagtttattg
     1981 tccctttctg tatcatcgaa aacagccatt ttttttgtgc ctatcacaac gaatttttgt
     2041 tctttgaatg ggtttaacca ggaaacaaaa atgtgtgctt tcacattatt gggaaaagaa
     2101 aggaaggtga gtgtgacatc ttccacatca tggggaagaa atatacctcc gtgggcgatt
     2161 acccttgctg gcaagcaacc agccagggaa agtatcaagg aaatatcgtg tggggcgaag
     2221 ctccacaaaa tgttttcttc gtgtcttatt cttccgatgt ttgttctatt tgaatacata
     2281 tattcaatgt cgccaagttc tttatttctt ataatctgct ttaatttttt tatcgcagga
     2341 tgataatgga gaatatggcc gaccattaat ataagatttt tttcttcagc aattcttgaa
     2401 aggtcttctc cctcctttac ggttaaggca agtggttttt cgcagaaaac atgtttatta
     2461 tgctccagcg atatttttgc tatactgtaa tgggtagatg caggtgttgc aatcactatt
     2521 gcatctatgt catttttttt gatgagttct tcaggctgag tggtaaaaaa aacatccgga
     2581 taattatttc tataatttgc catcaactct tcatttgaat cacatataaa tttaagcaca
     2641 ccaagctgat aaaaaactcg tatcaaattt ttgccccagt atcctccccc aataacccct
     2701 atttgtgctt tgtttgttat acttttgtcc tgacagtttt tatccattct tctatccttt
     2761 tgaattcttc aatcgtatta atatcacaac tttcttcagc cggtatttcc actgtctgaa
     2821 ctttcaaacc gtcttcaata tattctgcgt agacatctgt tagataatac tcacccgcag
     2881 cgttgttgtt ttttattttt cgcaactcat tataaattga tcttgattca caaacaatga
     2941 atcccgcgtt gacctcttta atttgaagcg tcgtttcatc tgcatccttt gcttctataa
     3001 cacctgcgaa tcttccgtct attcttatta ctcttccttt gcttcctgga ttaggtaaaa
     3061 ttgttgtagc tatagtaatg tctgcattgt tttgtatgtg ctggtcaaca agttttttga
     3121 acgttgctgt tgtaactaag ggtttatccg catatgttac gaggagtgtt cgatcagtgt
     3181 tttcaaaaat actgtgcgtg gacaaaagtg cgtgtcctgt tcccaggggc ctatcctgaa
     3241 ttacatattc aatattattg aaattgctaa ggatttgttt tgtctgacag gcatggggac
     3301 tgatcacaat aacaatttta ccaaccacat tttgaattgc tccaataatt ttagtgatta
     3361 atggtttacc ctgatactca acaagaaatt tatattgtgc ctgtccgtag cgagaacttt
     3421 ttccaccagc aagtatgatt ccagatatat ttttcattgc taaattatac aatgtatttg
     3481 ttcattttca actttgatta cattcagtat atatcgcggt gaaattgatt tcatgcaggt
     3541 atgatgctca cattgttttt tgtaacaggg agggcagaca ttattcgcct ggataaatgt
     3601 tattcctgaa gggcaatgat atcgtgggtc tgttggccca aagatagcaa ctgttctggt
     3661 accggtggca gccccgacat gcatgggacc actatctgtt gtgataagca atgctgcatt
     3721 ttcgataaca aaagccagtg taaaaaaatc tgtttttcct gataggtcaa taacatttgt
     3781 gcctatttta tttgctatca cggtgtttat tctcatggca tttttatctc ccacaagaac
     3841 gatatcaaac cctttttttg caagggacat tccaaggttt atccagtgat ttattggcca
     3901 gagttttgtt ttccacttgt gtgatgccga tgtatgaaat acaatgtatc gattaataac
     3961 cagtttatgt acatcaagat aattttttag attttttcgt ttttcatcag aaatccatat
     4021 ttgtaatttt ttcccaacag gtttgtttgt aaacttcttc cagaattgta cctgagattc
     4081 tattgcattg acattgtgaa gtttttttat gccctcagtg agaaaaagtt tgcctattgg
     4141 ttttttataa atacccactc tttgtttgct tcccagtaga aaggccagga aatttgtttt
     4201 caaatttccc tgtaagtcaa atataacatc aaactttttt ttacgaaggg ttcttacaat
     4261 gtttatgaat ggtaaagggt ttctggatgc cggcagagta aacacttttt tgatcatggg
     4321 acaatctttg tacatttcgg catatgatga actggtaaga agataaagtt gtgcatcagg
     4381 aaaagccatt gaaactgcat tgattgaaat agaaaaaagc gccatgtctc caaatgcgcc
     4441 tgttttcatc aagagtatct gtttcatgtt taacttagag taattcctga taaacctgta
     4501 cagttttttc caccatttgt tgaatggaga aatggttcat cacaaattct ttgccgtttg
     4561 ttgcaatgtg ctttgattcc tcaggatttt gaatgacgaa tataatcgct tttgccatcg
     4621 cttcaatatc acatggtgct gtgagtagtt ctgtttttgt tttgatgaat gttccttttt
     4681 ctataccatt cagggtagtt aaaactggaa tgcctgccag ctgtgcttca taaacactcc
     4741 taccaagcca tgggataatg ttttttttta atgtttcagt ttctatatgg ggcgccacaa
     4801 gcagatcaat ttcaggtaaa aagtcagatg cccttccctt caaaaataat gttgtgacac
     4861 caagtttttt accaagatat tcaagatttt ttttgtacgg gccatctcct atgatatgaa
     4921 tttttatgtt gggtacattt tgtttcgcaa tcgcgactgc ttgaataaga tactgaaatc
     4981 cttttaattt cgtcagtctt ccgatgcatc ctatgataaa tccagaatgt tttttataag
     5041 aaaggttaat atagggttca aatcttattc cattatgtac aacacgaaca cgatgtgggt
     5101 ctgctcccaa ttttttaatg gcaaattcac caagtgtatc gcttattaca attactcttt
     5161 cgcccttacc catgatttta ctatagaatg aggagccata aaatccatgt atacttgtta
     5221 cgaattttgt tttttgttgt tttgatgata gtgcataata accaatccat gcagggaatc
     5281 tgcttctaca gtgtaaaata tccggttttt catcggcaat aagtttttgt aaaactttca
     5341 gagatttcca aaaaataact ggtgtttttt
//
LOCUS       DAORYU010000090         5335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_10514_length_5335_cov_5.171402, whole genome shotgun sequence.
ACCESSION   DAORYU010000090 DAORYU010000000
VERSION     DAORYU010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5335
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10514_length_5335_cov_5.171402"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(296..1354)
                     /locus_tag="PLW07_04010"
     CDS             complement(296..1354)
                     /locus_tag="PLW07_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1646 family protein"
                     /protein_id="HOL49721.1"
                     /translation="MFITGQVIIILLVLLLPLLVHRVEKNLETFLFVMGVLSATISHF
                     SGPEHVWSLHLVEEALVEPIKITLAVCIAGFLFKRFRQSITKSIAHSEALLGPHVFAF
                     FFIVGLGLLSSIITAIIAALVLVEIINALPLDRKFETRLTIIACFAIGLGAALTPVGE
                     PLSTIATAKLKGPPYHADFFFLFKHLWFLIIPGVVVFGIIGAFLRAQQQNVNTQSKVL
                     QEETTKDILLRTGKVYLFVMALIFLGAGFRPVIDAYIVPLGDIALYWINTVSAVLDNA
                     TLASAEISPKMKLDQIQFALMGLLISGGMLIPGNIPNIIAASKLDIKSKQWAKFGLPV
                     GFAVLVIYFIAMLIFILA"
     gene            complement(1366..2511)
                     /locus_tag="PLW07_04015"
     CDS             complement(1366..2511)
                     /locus_tag="PLW07_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49722.1"
                     /translation="MWTPEHWYNDAKNWAQSHKDYMLKMYKLNLNLLKNHLPGTGERD
                     IYSLLKEHNQSRLNAPVDFKKYPELIGVKQLISAEWKGLQDGSGLDEYQTAVYVGAMN
                     FYHRMICSGKMGKMDGSLSAHCSYIFFPHSDHGVLLANNLDSSPDEPFGPPDWPALNE
                     HIIIGTVSSGIYLDEQPEEMFPAPVFKIVARYCRNAKEAVEALTRYNQFWGPCNAIVV
                     DRNHNCAMIEKSACRIGVRWSKDGFGFITAMTAEEPGMQAYLNERRQASLKARNLGPD
                     SPDAIYWKKQDQRRQLMNELLDQARKNPTLETMRTFIQYRDPVKGNVCGNGEIYGPGC
                     PPSEYTIRTIIWLLSECKALWWARQDNKPSFENPMPEVKFDSIIPWE"
     gene            complement(2547..5057)
                     /locus_tag="PLW07_04020"
     CDS             complement(2547..5057)
                     /locus_tag="PLW07_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49723.1"
                     /translation="MKKFFLSVIVSCLISVFAATGQTQNPPFKYVYATAYHVLPGTHN
                     NESGYFSLCEGLDGSIYIGTAKYNENSYLVEFDPYTETQKIVIDTHKVCGLNAKGYAA
                     QAKIHTRNFVGPSGKIYVGSKQGYRSSGDTSEYPGGYVMVYAPRTQQAESLDMPYPGE
                     GVIDVVADEQRHLIYVVTCENQHWMLYDQATKKYRELGPILLPYATTIVDKTGKAHAI
                     TNDFKIATYDPVKDTVLIRPVTVGRKVLTKPSGKGYAICFWVLAGDGKTAYMTMLSYP
                     DLYAIDLGSTGKTVKAQYLGKMIEGKNPDSRGSLCLHPDGNIYCLWRIDNDTGFGSGY
                     LHHLVRYNVKKKKMEDCGVIAVKNPSFYQFQNPDGTPKPFSHGFHKLPDGSLTILHVH
                     MAMIACRDGTLYATTLYPFTLLKIEDFKLPQKALTPKDKGYAATQYCKTVLDACDRVE
                     SNLSNIVEIAEIVAERHMKGGLIGFYPIVYQGLQDELWGRSGSMVNMGFDRPFKKDRT
                     EEEKKMDVSIIGWQTKPITKDEAKRIESFKAKGGFVIGFGPKDLVELEPQVKACNRWF
                     DTGTGTDDRCVVLGDGSKAGRTNHLLNALNAWALVAEIFSAITRKGHTPAMWKSYAYK
                     DGPEWGNQYLFKKQFMDEYPVTPIPEGKLAKRFLDGIRYHVRKFQNTQLSSIEKAVDM
                     IEKELRKTDRITIASMGHMPWTYVAKYEDARWAVNADLHSNVPHQVENYLKKTPDGAL
                     VVRLGYTGLDPDSKEIFKKKKQRLIVISAENDPFEFPDWNIPDDVCVYIDMGYAFGDA
                     CVAVEGLPIRILPPSGIMQIVAYECLNVEVLSRLVNRK"
BASE COUNT         1473 a         1106 c         1082 g         1674 t
ORIGIN      
        1 ggctgggatg gatttcaata aatccctctt gcatctccct tttataaagg gagaaaatgc
       61 ctgagaaatc ccccttagtc ccccttttgt aaagggggat atggaggaag gaattttgaa
      121 aaagggaaaa tgaaaagtag aaaaagaaga tctgagaaat ttcaaaaatc cacccatttt
      181 acaaaggggg aaggtgtgaa ggcgtattct gggaaatgtt tcttttgtat aatggagaga
      241 acgcaggaaa ttttcattgg aataatgaat gtaatgttta atcaaaaaac acatttcatg
      301 caagaataaa gataagcatt gcaataaaat atattacaag cactgcaaag ccaactggta
      361 atccaaattt tgcccactgt ttgcttttga tgtcaagttt gcttgctgcg atgatattgg
      421 gaatattgcc aggtatcagc attccacctg aaatcaatag tcccatcagg gcaaactgta
      481 tctggtcaag tttcatcttc ggactgattt cagcagatgc aagagtggca ttatcaagaa
      541 ctgcagagac agtgttaatc cagtaaaggg caatatctcc aaggggtaca atatacgcgt
      601 ctatcactgg tctgaaacca gcgcccagaa atattaaagc cataacaaaa agatatacct
      661 taccggttcg caacagtata tcttttgtgg tttcttcctg aagaactttg gactgtgtat
      721 ttacattttg ctgctgtgcc ctgagaaatg cgccaataat tccaaaaaca actaccccag
      781 gaataatgag aaaccagagg tgtttaaata gaaagaaaaa atcagcatga tatggtggtc
      841 ctttgagttt tgctgttgca atggttgaaa ggggctctcc cacaggcgtt aatgctgcgc
      901 caagtccaat ggcaaagcag gcaatgattg tcagacgggt ttcaaatttt ctatccaggg
      961 gcaacgcatt gatgatttca acaagaacaa gcgctgcaat aattgctgtg ataatgcttg
     1021 atagaagacc aagaccaacg ataaagaaga aagcaaaaac atgaggaccg agcaatgctt
     1081 cgctgtgtgc aatgcttttt gtaattgatt gcctgaatct tttgaacaga aagccagcaa
     1141 tacatacagc cagggtaatt tttattggtt caacgagtgc ttcttcaaca aggtgaagcg
     1201 accagacatg ctctggtcca gaaaaatggg agattgttgc agacagtact cccattacaa
     1261 aaagaaatgt ttcaagattt ttttcaaccc tgtgcacaag aagcggtaaa agtaatacca
     1321 gcaaaatgat gataacctga cctgtgataa acataattgt tccttttatt cccagggaat
     1381 aatactatca aactttactt caggcatcgg attttcaaat gatggtttgt tgtcctgcct
     1441 tgcccaccag agcgctttac attcgcttaa cagccatatt attgttctga ttgtgtattc
     1501 tgatggcggg caaccaggtc catagatttc tccattgcca cagacatttc cctttacagg
     1561 gtccctgtac tgaatgaatg ttctcatggt ttcaagggtt ggattttttc ttgcctggtc
     1621 aagaagttcg ttcatcagtt ggcgtctctg gtcctgcttt ttccagtaaa ttgcgtctgg
     1681 actgtcgggt cccagatttc tcgctttcaa tgatgcctgc cttctttcat tcagatatgc
     1741 ctgcatgcct ggttcttctg ctgtcatggc tgtgataaat ccaaacccat cttttgacca
     1801 tcttactcca atcctgcatg cgcttttttc tatcattgcg cagttatgat ttctgtcaac
     1861 aacaatagca ttgcacggac cccagaattg attgtacctt gttaacgctt ctactgcttc
     1921 ttttgcgttt ctacagtatc gcgcgacgat tttgaatact ggcgctggaa acatttcttc
     1981 tggctgttca tcaaggtaga tgcctgatga tactgtgcca ataataatgt gctcgttgag
     2041 tgcaggccag tcaggtggtc caaatggttc atcaggactt gagtcaaggt tgtttgcaag
     2101 aagtactcca tggtcactat gaggaaaaaa gatgtaactg caatgtgctg aaagagaccc
     2161 atccattttt cccattttgc cgctacaaat catcctgtga tagaaattca tggcgcctac
     2221 atacacagcg gtttgatatt catcaagtcc tgagccatcc tgcaatccct tccattctgc
     2281 actgattaac tgttttactc caattagttc agggtacttt ttgaaatcaa ccggcgcatt
     2341 gagccgtgat tgattatgtt ctttcagcaa actgtaaatg tcgcgttcac ctgtgcctgg
     2401 caggtgattt ttaagcaaat tcaagttaag tttatacatc ttcaacatat aatctttgtg
     2461 cgattgcgcc cagttttttg cgtcattata ccagtgttct ggtgtccaca catttactcc
     2521 tttttggatt taattatacc acaggattat tttctattta ctaaccttga aagtacttca
     2581 acattcaaac attcatatgc aactatctgc attatgccac ttggtggaag tatccttatt
     2641 ggaagtcctt ctactgccac gcaggcatct ccaaaggcat agcccatgtc tatgtaaaca
     2701 cagacatcgt ctggtatgtt ccagtcagga aattcaaatg ggtcattttc tgcgctgatc
     2761 acaatcaatc tctgtttttt ctttttgaag atttcttttg agtcagggtc aagtcctgta
     2821 taaccaagcc gaacaaccaa tgcaccatca ggtgtttttt tcaggtaatt ttcaacctga
     2881 tgcgggacat tgctgtgaag gtcagcattc actgcccatc gtgcgtcttc atattttgcc
     2941 acatatgtcc agggcatatg tcccattgag gcaatggtaa ttctatcagt ttttcgtaat
     3001 tctttttcga tcatatcaac tgccttttcg atgctggaca actgtgtatt ttgaaatttt
     3061 cttacatggt acctgatgcc atcaagaaat ctttttgcca gtttgccttc tggtattgga
     3121 gtcacaggat attcatccat aaattgtttt ttgaaaaggt attgatttcc ccattctgga
     3181 ccatccttgt aggcgtatga cttccacatt gctggcgtat gcccttttct tgtgatggca
     3241 gagaaaattt ctgctacgag tgcccaggca tttaaagcat tgagcagatg atttgttctt
     3301 cctgcctttg aaccatcacc aagtaccacg catctatcat cagtgcctgt gcctgtgtca
     3361 aaccatctgt tacacgcttt cacctgtggc tcaagttcaa caaggtcttt tggtccaaat
     3421 ccaatgacaa atccaccttt tgccttgaat gattcaattc tttttgcttc gtcttttgtg
     3481 attggttttg tctgccagcc aatgatagat acatccatct ttttttcttc ttctgttcta
     3541 tcttttttga atggtctgtc aaatcccata ttgaccatac tgccactacg accccacagt
     3601 tcatcctgaa gcccctgata cacaatggga taaaagccaa taagaccgcc tttcatgtgt
     3661 ctttctgcaa caatttctgc aatctcaaca atattggaaa gatttgattc aaccctatca
     3721 caggcatcaa ggactgtttt gcagtactgg gttgctgcat atcctttatc tttcggtgtg
     3781 agcgcttttt gtggaagttt gaaatcttca attttcaata aagtgaatgg ataaagggtt
     3841 gttgcgtata atgttccgtc acgacatgca atcatcgcca tgtgaacatg aagtattgtt
     3901 aacgagccat caggaagttt gtgaaaccca tgtgaaaatg gttttggcgt gccatcaggg
     3961 ttttggaact ggtaaaacga tggatttttc actgcaatca ctccgcaatc ttccattttt
     4021 ttctttttta cattgtatct gacaagatga tgcaggtatc cagagccaaa accagtgtca
     4081 ttgtcaatcc tccacaggca ataaatgttt ccatcaggat gaagacacaa agagcccctt
     4141 gaatcaggat ttttgccttc aatcattttg ccgaggtatt gtgctttgac tgtttttcca
     4201 gtacttccaa ggtctattgc ataaaggtca ggataactga gcattgtcat ataggcggtt
     4261 ttaccatcac cagcaagcac ccagaaacaa atggcgtatc ctttgccaga tggtttggtc
     4321 agaacttttc ttcctacagt aacgggtctg attaatacag tatccttcac agggtcatat
     4381 gttgcgattt tgaagtcgtt tgtgattgcg tgcgctttac ctgttttatc aacaattgtt
     4441 gtcgcatagg gcaaaagaat tggtccaagt tctctgtatt tttttgttgc ctggtcgtaa
     4501 agcatccagt gctgattctc gcatgtgaca acatatatga gatgtctttg ttcgtctgca
     4561 accacatcaa tgacaccttc gccaggatac ggcatgtcaa gggattctgc ctgttgagtg
     4621 cgcggggcat acaccatcac ataaccgcca ggatattcag atgtatctcc tgaactgcga
     4681 taaccctgtt tgcttcccac ataaattttt ccgcttggac ccacgaaatt tcttgtgtgg
     4741 atttttgcct gtgctgcata gccctttgca ttcagtccac aaaccttgtg ggtatcaatt
     4801 acaatctttt gtgtctctgt atacgggtca aactctacaa ggtaggaatt ttcattgtat
     4861 tttgctgtgc caatataaat actcccatca agaccttcac agagcgaaaa gtagcctgat
     4921 tcattgttat gagtaccagg aaggacatgg tatgctgttg cataaacata cttaaaaggc
     4981 gggttctggg tttgtcctgt tgcagcaaat acagaaatca aacatgatac aattacagag
     5041 agaaagaatt ttttcatttt gctccttatt ggtgaaataa tataatgcac aggaataaaa
     5101 ttgtaaagca ggtaaactcg cagtttcaaa ggaggagttt tcaacccttc aaataatcct
     5161 tggtttaggg attcgcactg ttgtaatgcc cccatttgtc aagagcaaaa taagggagaa
     5221 tttatactgc tccccattaa gtggagatgg aaaataaggt agaatagagt ggtctgaaag
     5281 ggggggcaga aatggaacgg aggaaatggg aaagcaagca gaagtttgag atagt
//
LOCUS       DAORYU010000091         5312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_10570_length_5312_cov_4.887769, whole genome shotgun sequence.
ACCESSION   DAORYU010000091 DAORYU010000000
VERSION     DAORYU010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10570_length_5312_cov_4.887769"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(230..556)
                     /locus_tag="PLW07_04025"
     CDS             complement(230..556)
                     /locus_tag="PLW07_04025"
                     /inference="COORDINATES: protein motif:HMM:NF021390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HOL49724.1"
                     /translation="MKKLTIWKCLVMMIPAMFIAGCAGLQTKGNIKVWYVREDGKKMH
                     VTFVKNPAQAILNLGKGNVLHLKQVVSGSGSRYSDGKTEFWIKGDQATLTISGKVIKC
                     WVENNH"
     gene            647..1681
                     /locus_tag="PLW07_04030"
     CDS             647..1681
                     /locus_tag="PLW07_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49725.1"
                     /translation="MRKIYAAIIFSFSVFIFPSYGHLCDNVFRQADKLIVKPETYNIV
                     VKDKTTFKIFLQNNMDRGIAEIRLLAESPAFDFQIYPEKMMIPKDQRVYFEVTMIPKP
                     STSSGNYPVTFRLVGGGRQFKSFSLDIAGDKKDSGEIKKEKQETRQAPATKTQSILPV
                     RRANQSPKIDGRIVEECWKNAGVAGNFCLFNQGKPVYQTVGLLAYDTEAMYLAFYCRE
                     GTPEKISDKDKIEIQISPDQTGKSYYSITLTGDEKIFCKRTGFNGESQTVSINGLRYA
                     ISRGVTAWMVEIAIPFSSIGAKAPEIGNIWALRIIRTKATQSPEESFWAVDASSYHQE
                     KGFGQVVFAQ"
     gene            1693..2232
                     /locus_tag="PLW07_04035"
     CDS             1693..2232
                     /locus_tag="PLW07_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49726.1"
                     /translation="MNKFILIVLLACVFTGRAEQIGVNFEYPDVEGDFYLAGTVFFPP
                     SSILSINNICVLDTNTKKEIASKIVVVEKWPDDSIMSVEIIFPANSQKKTEYAISYGS
                     DVKRRKTFSEPAVLPTVSFFPAGTGRSAENVDVSVGQINVRVDKSASVRYWWYIVPMV
                     ILLFLTVYRSVRTIRTQKL"
     assembly_gap    2367..2376
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2377..3284
                     /locus_tag="PLW07_04040"
     CDS             <2377..3284
                     /locus_tag="PLW07_04040"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOL49727.1"
                     /translation="TTQFAKGSILWPSGATPVIENYPPSLVCRFNPKGGMFKACILHF
                     FSENLTWQTSFKYLLPYITLFIALCFLLGRVWCGWVCPIGTVGDFLTYLRRKMNIPVR
                     RFPERFRFNLSFTGYSLMVIALVISTVIGMPNYARFQCYWFLPYCQICPARLICPMFG
                     LIKPNWKDFSTGITTSFTILAWIVLGLFVAAFYFGRRVWCHMCPVGLINSWFNKGAGI
                     ELRKKAIKCIKCGYCADACPMGLTAMYELNRDAIYNQTGCIMCMRCVEVCPRKGCLRV
                     SFFGKQIGNTKRRKLDAKKVSEPVQ"
     gene            3253..4494
                     /locus_tag="PLW07_04045"
     CDS             3253..4494
                     /locus_tag="PLW07_04045"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOL49728.1"
                     /translation="MQKKFLSQYSEMLLRNLKCNHPDSDNIAAVILKYMKKSNISSME
                     GFYQAIVDFERNGMDTREKPIGFFCSVVPEEIMLACGLRPVRLCCEEYGCWSTGEEVV
                     PGDVCPVIKAVCGKFYSGACDKLETVVIPGTCDPKAKLAEILSPIKQVYFLDTGRDSE
                     YLNNVDIWEKKYAQFFQFLKEKFYRNAGRSQLLSACKLTNSRTEIFRNIYQLRAERPD
                     AINVFDYYIMIYAGFFMDVKKWSTLAEKVYQQAKQIYRLDNTPRILLAGTPVIFPNFK
                     ILDVMEDSGLKMAADLQCTSFGRFYNPVMIDEETESGILRALTLKNIAGSMCPCMLNL
                     EKLVNLMIDTVNQYKLDGVIYYNLRLCQVFELQTALIRQILKEKNIPFLSIKTDFGKE
                     DTGQLKTRMEAFREMLESRNG"
     gene            4506..5264
                     /locus_tag="PLW07_04050"
     CDS             4506..5264
                     /locus_tag="PLW07_04050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOL49729.1"
                     /translation="MLKAGIDMGSVMVKCVIVNGETIITKSAARSGPMPEIIAETVLK
                     QALENAGIDVSLIERIICTGYGRHIFSKAHATVTEISAAARASFLLSGRQRCLVIDVG
                     GQDTKIIEVDNNGEVIDFLMNDKCAAGTGRFLEMMAGVLNTDLTGLGTLSLKAEKPVI
                     INATCSVFAESEVISLLAHNEKKENIAAGIFNAIAARISTMLNHFQGNIPVFFCGGPA
                     EIPGMKMAIEKISGRNIKIFDNPEFVVAYGAALG"
BASE COUNT         1558 a          977 c         1195 g         1572 t
ORIGIN      
        1 gtatccggtt ccaggggcgc atccaggtgt tgccataatc cactcacagt ttgccctcca
       61 cagggttttt gtttctcttg ccagtttctc catgtctgca tcaagaaatt tgctgaaatc
      121 tccaagataa tcacgtctca tcatcctctg cgggctttcc caccattgtt ttcgcatggt
      181 tcctccatgg tgattttgta aaaaatgaga tatacaggag gcaggttgtt caatgattgt
      241 tttcaaccca gcatttgatt acttttcccg agattgttag tgttgcctgg tctcccttaa
      301 tccagaattc agtttttcca tcgctatatc gtgagcccga acctgaaaca acctgtttca
      361 gatgcaacac attccctttg ccaaggttca aaatcgcctg agccggattt tttacaaatg
      421 tcacgtgcat cttttttcca tcctcacgca cataccagac cttgatattg cccttagttt
      481 gtaatccagc acagccagca atgaacatgg caggtatcat catcaccagg catttccaga
      541 tggtcagttt tttcataggc ctcccttttc catatcataa acctgatagt attatgagtc
      601 aatgatgtta ctggacggaa agtatttttt gggttaaact cactgcatga gaaaaatata
      661 tgcggcaata attttttctt tcagcgtttt catttttccc tcctatggac atctctgtga
      721 taacgtgttc cgtcaggcag acaaactcat tgttaagcca gaaacatata acattgttgt
      781 gaaagataag acaacattca aaatttttct acagaataat atggacaggg gcattgcaga
      841 aataaggttg cttgctgaga gccctgcatt tgactttcag atttatcctg aaaaaatgat
      901 gattccaaaa gaccagaggg tatattttga agtaacgatg attccaaaac cttctacctc
      961 atcagggaat taccctgtta ctttcagact ggtcggcggt ggaaggcagt tcaaaagttt
     1021 cagccttgac atagcaggag ataaaaagga ttctggtgag ataaaaaaag aaaaacagga
     1081 aacccgtcag gcgcctgcaa cgaaaacaca atctatcctt cctgttcgca gggcaaacca
     1141 atctccgaaa atagatggaa ggattgttga agaatgctgg aaaaatgctg gtgttgcagg
     1201 aaatttctgt ttgttcaatc agggcaaacc tgtttatcag accgtcgggt tacttgcgta
     1261 tgatacagaa gcaatgtatc ttgcatttta ctgcagggaa gggaccccgg agaaaatttc
     1321 ggataaggat aagattgaaa tacagatatc gcctgaccag acaggaaaaa gttattattc
     1381 aattaccctg acaggcgatg aaaaaatttt ctgcaaaagg actggtttca acggagagtc
     1441 ccagacagta tccatcaatg gattgagata tgccatatca cggggtgtga ctgcatggat
     1501 ggttgaaatt gcaattccct tttcttctat cggagcaaaa gcccctgaga taggaaatat
     1561 ctgggcgttg agaatcatac gaacaaaggc aacgcaatct cctgaagaaa gtttctgggc
     1621 tgttgatgca tcgtcctatc atcaggaaaa agggtttggt caggttgttt ttgcgcaatg
     1681 atgcatattc cgatgaacaa attcattctt attgtactgc tggcatgtgt tttcactggc
     1741 agggctgaac agattggcgt taattttgaa tatcctgatg tggaagggga cttttatctt
     1801 gccgggactg tttttttccc gccgtcttct attctgtcta taaacaatat ctgtgtgctg
     1861 gataccaaca cgaaaaaaga gattgcttca aaaattgtgg ttgtggaaaa atggcctgat
     1921 gattcaatca tgagtgtgga gattattttt cctgcaaaca gtcagaaaaa aacagaatac
     1981 gcaatatcgt atggaagtga tgtcaaaaga agaaagacct tttctgaacc tgcggttctt
     2041 ccaacagttt ccttttttcc tgcaggaacc ggaaggtctg cggaaaatgt tgacgtatca
     2101 gtcggtcaga ttaatgtgag ggttgataaa agtgccagtg tcagatactg gtggtacatt
     2161 gtacccatgg tcattctttt atttttaact gtttacagaa gcgtcagaac aataaggacc
     2221 cagaaattat gatacgcatt tcagttgcaa gaagaattgt tcaacttatc tgttttgtgc
     2281 ttcttgttta tggtggttat atttttaagg actggaaaga atctgctgaa accagtcagc
     2341 agtcgatttt cccgtcgctc aaagcannnn nnnnnnctac aacacagttt gcaaagggaa
     2401 gtattttatg gccatcaggg gcaacgcctg tgattgaaaa ttatccgccc tcacttgtgt
     2461 gcagatttaa tccaaaagga ggcatgttca aggcatgcat cttgcacttt ttttcagaaa
     2521 acctcacctg gcagacatct ttcaaatatc tgttgccgta tatcacgctt tttattgctc
     2581 tgtgcttcct gctgggaagg gtgtggtgcg gttgggtatg tccaatcgga acagttggag
     2641 atttcctcac atatctgcgc aggaaaatga atattcctgt tagacggttt ccagaacgat
     2701 tcaggttcaa cctttctttc acaggatatt ccctgatggt gattgcgctt gttatttcaa
     2761 ctgtcatagg tatgccaaat tatgctagat ttcaatgcta ctggttcctt ccttactgtc
     2821 agatatgtcc tgcaagattg atatgcccga tgtttggtct gataaagccg aactggaagg
     2881 acttttcaac cggtatcacc acttctttca caatccttgc ctggattgta ctggggcttt
     2941 ttgttgctgc gttttatttt ggaagaaggg tctggtgtca tatgtgtcct gtaggattga
     3001 taaattcatg gttcaataaa ggagcaggaa tagaactgcg taagaaagcc atcaagtgca
     3061 tcaaatgtgg atattgcgcg gatgcctgtc caatggggct gacagcaatg tacgagttga
     3121 acagggatgc catttataat caaacaggtt gtattatgtg tatgagatgc gtggaggtat
     3181 gtccgagaaa gggctgtctg agagtcagtt ttttcgggaa acagattggc aacacaaaaa
     3241 ggagaaagtt ggatgcaaaa aaagtttctg agccagtaca gtgaaatgtt gcttagaaat
     3301 ctgaaatgca atcatcctga ttctgacaat attgcagccg ttatccttaa gtatatgaaa
     3361 aaatccaata tttcttcaat ggagggattc tatcaggcaa ttgtggactt tgaaagaaat
     3421 ggtatggata ccagggaaaa acccattggt tttttttgca gtgttgttcc tgaagaaatc
     3481 atgcttgcct gcggcctgcg acctgtaagg ctgtgctgcg aagagtatgg atgctggtca
     3541 acaggggaag aagttgtacc tggagatgtt tgccctgtca tcaaggcggt ttgcggtaaa
     3601 ttttatagcg gtgcctgcga taaactggaa actgttgtta ttccaggcac atgtgatccg
     3661 aaggcaaaac ttgcagagat tctctcacct ataaaacagg tatattttct tgataccgga
     3721 agggactctg aatacctaaa taatgttgac atatgggaaa aaaaatacgc acagtttttc
     3781 cagtttttga aggaaaaatt ttatcgaaat gcaggaagga gtcagttact ttctgcctgc
     3841 aaactcacaa actcacgtac tgaaattttc agaaacattt atcagttaag agcagaacgg
     3901 ccggacgcga ttaatgtgtt tgattattac atcatgattt atgcgggttt tttcatggat
     3961 gttaaaaaat ggagcaccct tgcagaaaaa gtttatcaac aggcaaaaca aatttacagg
     4021 ttggataata cacccagaat tcttcttgct ggaacacctg tgatatttcc taattttaag
     4081 attcttgatg taatggaaga ttctggattg aaaatggcag cagaccttca gtgtacatca
     4141 tttggaagat tttataatcc tgtgatgata gatgaagaaa cagaatcagg tatcctgcgg
     4201 gctttaacat tgaaaaatat cgctggcagc atgtgtccgt gtatgctgaa tcttgagaaa
     4261 ctggtcaatc ttatgattga tactgtgaac cagtacaaac ttgatggtgt catctattac
     4321 aatttaagac tatgccaggt ttttgaactg cagacagcat taatcaggca aatccttaaa
     4381 gaaaaaaaca taccgttcct ttcaataaaa accgactttg gaaaagaaga cacaggtcaa
     4441 ttgaaaacaa gaatggaagc attcagagaa atgcttgaat cccgcaatgg atgaagggag
     4501 gatatgtgtt gaaggcaggt attgatatgg gttctgtgat ggtcaaatgc gtaattgtta
     4561 atggtgagac aatcatcacg aaatccgcag caaggtcagg tccaatgccc gaaattatcg
     4621 ccgaaacagt tctgaagcag gcgttagaaa acgcagggat tgatgtttct ctcattgaaa
     4681 gaattatatg tacaggttat ggcaggcaca tcttctcaaa ggcgcatgca accgtaacag
     4741 agatttctgc tgcagcaagg gcatcctttt tgttgtctgg aagacagaga tgtcttgtga
     4801 ttgatgttgg tggtcaggac acaaaaataa tagaggtgga taacaatggt gaggtcatag
     4861 attttttaat gaacgacaag tgtgcggctg gcacaggaag gtttcttgaa atgatggcag
     4921 gtgttttaaa cacagacctt accgggcttg gaacactttc cctgaaggca gaaaagccag
     4981 tgatcatcaa tgctacatgc agcgtttttg cagaaagtga ggtgatttcg ttacttgccc
     5041 ataatgaaaa aaaggaaaac attgctgccg ggatttttaa tgcgattgcc gcgagaattt
     5101 caaccatgtt gaaccatttt cagggtaaca ttccagtttt tttctgtggt ggtccagcag
     5161 agataccagg catgaaaatg gcaattgaaa agataagcgg aaggaacatt aaaatttttg
     5221 ataatcctga gtttgtggtt gcttatggtg ctgcgctcgg ttgacaggat gaaaaagttt
     5281 taataaaatt atttggtcta tttttttacc aa
//
LOCUS       DAORYU010000092         5262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_10702_length_5262_cov_4.185327, whole genome shotgun sequence.
ACCESSION   DAORYU010000092 DAORYU010000000
VERSION     DAORYU010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5262
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10702_length_5262_cov_4.185327"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1415
                     /locus_tag="PLW07_04055"
     CDS             <1..1415
                     /locus_tag="PLW07_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOL49730.1"
                     /translation="LKGSCFYLTSGATKLLEERTIKKLERKLHLSITDINRIIIDIAV
                     KNGKNLELLTDSDFHQDKKGRFLYNKEKDILSLTRNVSALFKERIEKGTKTIENCVFL
                     HTGPHHDDIMLGYLPYIIHLVRTPLNKHYFATMTSGFTSVTNDYIVSQLKNLENFIDT
                     KQFKQLFESEYFNPDDGVARIRDVYHYLDGVALNSTDIQNEAMARRMLRNIVSIVNSY
                     DNQVIKRKISWIFRYFESSYPGKKDIPDVQLLKGMIREWEEELLWGHLGFNCDHIFHL
                     RLPFYTGDIFTKQPQLNEDIVPILSLIEKLNPDVITVAMDPEASGPDTHYKVLQAIAE
                     ALALYLKKNPNKSMRVWGYRNIWYRFHPAEADIMVPVSMNSFAIMNSAFDICFGSQRS
                     ASFPSYEYDGPFCALAQKIMAQQYTVLKTCLGRDFFNSNEIARLRATRGLVFLRDMSP
                     DEFFSEARYLKQLTESSQDD"
     gene            1429..2820
                     /locus_tag="PLW07_04060"
     CDS             1429..2820
                     /locus_tag="PLW07_04060"
                     /inference="COORDINATES: protein motif:HMM:NF014649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49731.1"
                     /translation="MARKPRIGMMGVTAQLYKKKIPEFVDNLSEQFTCFIQKCSHFAD
                     IIPSEISYTEELVEKSYTKMSQSDVDGIIIIFLSYSPSLIIEPVIKSRKLPVLIWNTQ
                     QLYEITGDFSSQDMMNNHGMHGVQDIASVLLRNDVPFTVITGHHSQKDTVQKIEYWCK
                     CAAIRHDLKKLNIGRIGGRFKDMGDFAISDKQITDVIGPKIIEIKMSELNKAANSISP
                     EEIVKVINQENKKFLVEKNLDNETREKSVRLELALRKLIDLYHLEALAINFMGFEGKN
                     CTMPFAAISKFISEGVGYGGEGDVISATAVWVLQQIAPPATFTEMFTTDYKNNRIFMS
                     HMGESNFKMAKNPLNIKLVKKDMGILEKGTATSMFLFQMKPGYVTLFNLAPCRKGNFR
                     MIATTGYIEDIELFQDITSPHFLLKIANDVREFLSQYSNNGGTHHLAMVYGDVIEQIR
                     ILASIMGLEFSKI"
     gene            2826..4208
                     /locus_tag="PLW07_04065"
     CDS             2826..4208
                     /locus_tag="PLW07_04065"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOL49732.1"
                     /translation="MDRILSFDSGTTNTKVFVYSQNGVLVNSSSRQIDITFPYPLFAE
                     QNCEDWVSALTRAITKIADKKNISGISGSFQGGTFVLLDKHLLPLRPAITWLDNRGRD
                     ISKNFVARFGRKFFYNKTGFVPGGWSCVALIRWLKENEPDVFNKVARISFVADYINYF
                     LTGNFVLDHTNAAITTLYNIKKRQWDDEIIGLAGINKNMLPEIVDAPNIIGKLKKSFA
                     EDTGLKEGISVLAGGHDQYCASLGAGAAEKGKILVSCGTAWVLLAATRDIVFDRLMQF
                     APGPHVVPGIHGLMAALSNGGIVYAWGKNILRKHPVSFNKPSSVIVVPDFNTSNGAIC
                     NLSLSTKPREVLQAIVESLCFQVRHSLDRIGKILPEGEEISQIVMIGGAAKDIHVANL
                     LSTITKKQVFLPEIKEAAGFGAMSLFNLRHKCKINLSGVVIEGDKNLHHTYDEMYRNY
                     IGLRNLLLKE"
     gene            complement(4182..>5262)
                     /locus_tag="PLW07_04070"
     CDS             complement(4182..>5262)
                     /locus_tag="PLW07_04070"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOL49733.1"
                     /translation="LIAIKYGATSQQLGILGSVTALFYIPFSILFGVLAGKQLKVNFP
                     LISATIFSLFIAMTMLSNSVRVLYFIAAAAGISLAGFWPPLMVQMNKETEGKNTGEIV
                     GKFSAAWSIGTIFGPIVAGFLYQQYILAPLMLSAFLFLCIVFILSTVKSSPSIVKKIT
                     QDNKKDTPVSDIKTLVAAYIGTFAVYFSFGAVRNLFPKLAVDIGLQPYVIGLLFSIMD
                     TFRTITFFVFSQREHFYQKPFSFVAFGFFAYFSLSWLIFTNSVLLFAMNFILLGGILC
                     GIYYSYALHTAFTIKKGRAIAVGIFEAVIGIGVGSGSLVGGMLTFWLGERAPYIVSMI
                     IGIVFSVIQITLVRNKNSLLQKKVS"
BASE COUNT         1744 a          861 c         1104 g         1553 t
ORIGIN      
        1 aactgaaagg ttcttgtttt tatcttacat ctggcgcaac aaaacttctt gaggaaagaa
       61 ccataaaaaa acttgaaaga aagctgcatc tttcaatcac tgatatcaat cgtatcatta
      121 ttgatattgc ggtaaaaaat ggtaaaaacc tcgaattgtt gactgattca gattttcatc
      181 aagataaaaa gggcagattt ctttataata aagaaaaaga tattttatct ttgaccagaa
      241 atgtgagtgc actttttaag gaaagaatag aaaaaggtac caaaactatt gaaaactgtg
      301 ttttcttgca tactggacca catcatgatg acattatgct cggatatttg ccctatatta
      361 ttcatcttgt aaggacgcct ttgaacaaac attattttgc aactatgaca agcggtttta
      421 catcagttac caatgattac atagtttccc agttaaaaaa tctggagaat tttattgaca
      481 caaaacagtt taagcaatta tttgaatcag aatattttaa tcccgatgat ggtgttgcga
      541 gaatcaggga cgtttatcat tatcttgacg gtgttgcgtt aaatagtact gatattcaaa
      601 atgaagcgat ggcaagaaga atgttgagaa atattgtttc aattgttaat tcatatgata
      661 atcaggtgat aaaaagaaaa atttcctgga ttttccgata ttttgaaagt tcatacccag
      721 gtaagaaaga cattccagat gttcaactac ttaagggtat gataagggaa tgggaagaag
      781 aacttttatg ggggcatctt ggatttaatt gcgaccatat ttttcatctg agattgcctt
      841 tttatacagg agatattttt acgaagcagc cccagttgaa tgaagatatt gtacccatac
      901 tttctttgat tgaaaaactc aatcctgatg ttattacagt ggcgatggat ccagaggcaa
      961 gtggtccgga tacgcattat aaagttcttc aggcaatagc agaagcactg gcactgtatt
     1021 tgaaaaaaaa tcccaacaaa tcaatgcgag tatggggata tagaaatatc tggtacagat
     1081 ttcatccagc agaagcagat attatggttc ctgtatcaat gaattcgttt gcaatcatga
     1141 atagcgcttt tgatatctgt tttggctcgc agagaagcgc atcattccca agctatgagt
     1201 atgatggacc attttgtgcg cttgctcaga aaattatggc acaacaatat acagtgttaa
     1261 aaacatgtct gggtcgtgat ttctttaatt caaatgaaat tgcaagatta agggcaacaa
     1321 gaggtcttgt gtttttaagg gatatgagtc ctgatgaatt tttcagtgaa gcacgatatc
     1381 tgaaacaact gactgaaagt tctcaggatg actgaacgga ggtaccctat ggcgagaaag
     1441 cccagaattg gtatgatggg tgtcactgct caactttata aaaagaaaat accagaattt
     1501 gtggataatc tttctgaaca attcacatgt tttattcaga agtgttcaca ttttgcagac
     1561 atcattcctt cagaaatatc ttacacagag gaactggtag aaaaatcata tacgaaaatg
     1621 agtcaatccg atgtggacgg tattatcatt atatttcttt catacagtcc cagtcttatt
     1681 attgaacctg taatcaagtc cagaaaactt ccggttctta tatggaatac ccagcaactg
     1741 tatgaaataa cgggagattt ttcatcgcag gatatgatga ataatcacgg tatgcatggt
     1801 gttcaggata tcgccagtgt tttgttgaga aatgacgtgc catttactgt tattacggga
     1861 catcattccc agaaggatac tgttcagaaa attgagtact ggtgtaaatg tgcagcaatt
     1921 cgtcacgatt taaagaaatt aaacataggc cgtattgggg gtagattcaa ggacatggga
     1981 gattttgcaa tttctgataa gcagattaca gatgttatcg ggccaaaaat cattgaaatc
     2041 aaaatgtctg agttgaataa ggcagcaaac agtatctcgc cagaagaaat cgtaaaagta
     2101 attaaccagg aaaataagaa atttcttgtg gaaaaaaatc ttgataatga aacaagagaa
     2161 aaatcagttc gtctggaact tgcactaaga aaattaattg acctgtatca ccttgaagca
     2221 ctggcaatca attttatggg gtttgaggga aagaattgta ctatgccttt tgcagcaatt
     2281 tcaaaattta tttctgaagg cgtgggatat ggaggagaag gagatgttat cagtgcaaca
     2341 gcagtatggg ttcttcaaca gattgcgcca cctgccacat ttacagaaat gttcacaact
     2401 gattataaaa acaacagaat ttttatgtca cacatggggg aatcaaactt taagatggcg
     2461 aaaaatccat tgaatataaa acttgtaaaa aaagatatgg gtattctgga aaaaggaact
     2521 gctacaagca tgtttttatt tcagatgaag ccgggttatg tgacgttatt caatcttgct
     2581 ccatgcagga aagggaactt tcgtatgata gcaactaccg gatatattga agatatagaa
     2641 ctttttcagg atattacatc tccgcatttt ctgctgaaga ttgcaaatga tgtaagagaa
     2701 tttttgagcc agtatagcaa taatggaggt acccatcatc ttgcaatggt ttatggagat
     2761 gttatagagc agattagaat ccttgccagt atcatggggc ttgaattttc aaaaatataa
     2821 cgaacatgga tagaatcctt tctttcgata gtggcacaac aaatacaaag gtttttgttt
     2881 attcacagaa tggtgttctt gttaatagtt cttcaagaca gattgatatc acatttccct
     2941 atcccttatt tgctgaacag aattgtgagg attgggtcag cgcattaacc cgggcgataa
     3001 cgaaaatagc agataaaaaa aatatcagtg gtatcagtgg aagtttccag ggcggtactt
     3061 tcgtgttgct tgataagcat cttttgccat tgagaccggc aataacatgg cttgataaca
     3121 ggggcaggga tatctcaaaa aattttgtgg ccagattcgg tagaaaattt ttttataata
     3181 aaacaggatt tgtaccaggt ggctggtcct gtgttgccct tatcagatgg ttgaaagaaa
     3241 atgagccaga tgtatttaat aaagttgctc gcatctcatt tgttgcggac tacatcaatt
     3301 attttctgac cggaaatttt gtgcttgacc atacgaatgc cgcaattacg acgttgtaca
     3361 atatcaaaaa aagacagtgg gatgatgaaa ttattggact ggcgggaatt aacaagaata
     3421 tgcttcctga aattgttgat gctcccaaca ttataggaaa attgaaaaaa agttttgctg
     3481 aagatactgg attgaaagaa ggcatttctg tgcttgctgg tgggcacgac cagtattgtg
     3541 caagtttggg agcaggggct gcagaaaagg gaaagattct tgtttcatgt ggaactgcct
     3601 gggttctgct tgccgccaca cgtgacattg tttttgaccg tttgatgcag tttgcccctg
     3661 gtcctcatgt ggtaccaggt atacacgggc ttatggcggc tttgagtaat ggaggcattg
     3721 tgtatgcatg gggaaaaaat attttgcgaa agcatcctgt ttcttttaat aagccatcat
     3781 ccgttattgt ggtacctgat tttaatacta gcaatggtgc tatttgtaat ctcagcctgt
     3841 ccacaaaacc ccgtgaggta ctgcaggcaa ttgttgagtc cctttgtttt caggtgagac
     3901 acagtcttga tagaataggt aaaattttgc ccgaaggaga ggaaataagt cagattgtga
     3961 tgataggcgg cgcagcaaag gatattcatg tggcaaatct tttatccacc atcacaaaaa
     4021 agcaggtttt tttgccagaa attaaagagg ctgcaggttt tggagcaatg agtcttttta
     4081 acctgcgtca caaatgcaaa ataaaccttt ctggtgttgt catagaaggg gataaaaatt
     4141 tgcatcatac ttacgacgaa atgtacagaa attatattgg attaagaaac cttcttttga
     4201 aggagtgaat ttttgtttct taccagtgta atctgaatga cagaaaatac tattccaata
     4261 atcattgata ctatgtatgg ggctctttct ccaagccaga aagtaagcat accaccaacc
     4321 agagaaccac tgccaacgcc tattccgatg actgcttcaa aaattccaac agcaattgca
     4381 cggcctttct ttatcgtgaa tgccgtgtga agggcatagg aataatagat tccgcataaa
     4441 attccgccaa ggaggataaa attcatcgca aatagaagga ccgaattggt aaaaattaac
     4501 catgaaaggg aaaaataggc aaaaaagcca aaagcaacga atgaaaaagg tttttgatag
     4561 aaatgttccc tctgactgaa aacaaaaaat gttatggttc tgaaggtatc catgattgaa
     4621 aacaataaac caattacata aggctgtagc cctatgtcaa cagcaagttt tggaaaaaga
     4681 tttcttacag cgccaaagga aaagtaaaca gcaaatgttc caatatatgc tgcgacgagt
     4741 gtttttatgt ctgaaacagg tgtatctttt ttattatcct gtgttatctt ttttacgata
     4801 gagggactgg attttacagt actcaggata aaaacgatac agaggaaaag aaaggcggat
     4861 aacattaatg gagcaagaat atattgctga tatagaaatc cagcgactat cggtccaaag
     4921 attgttccaa tactccaggc agcactgaat tttccaacaa tttcacctgt attttttcct
     4981 tcggtttctt tgttcatctg caccatcagc ggtggccaga atcctgcaag agaaatgcct
     5041 gcagcagcgg ctataaaata caggacacgt actgaattac tcagcatagt cattgcgatg
     5101 aatagagaaa atattgtagc cgatatcagt gggaaattaa ctttcaattg tttaccggca
     5161 agaacgccga ataaaatcga aaatgggata tagaacaaag cggtgacact accaagaatg
     5221 ccaagttgtt gagaggttgc accatatttt attgcaatga ga
//
LOCUS       DAORYU010000093         5199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_10874_length_5199_cov_5.078344, whole genome shotgun sequence.
ACCESSION   DAORYU010000093 DAORYU010000000
VERSION     DAORYU010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10874_length_5199_cov_5.078344"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="PLW07_04075"
     CDS             complement(<1..695)
                     /locus_tag="PLW07_04075"
                     /inference="COORDINATES: protein motif:HMM:NF021882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOL49734.1"
                     /translation="MDKDTICALSTPYGISGIGIIRLSGPETFQIVKKIFKPARRRKL
                     PSVWETHTVRYGHIVDGEKIVDEVLVTIMKSPSSYTREDMAEIGCHGGIAAIKSVIAL
                     CLRNGARFAEPGEFTKRAFLNGRIDLAQAESILEVINAKTEKSLEIAVNQLTGSLSVR
                     ISEFRKKLLNILTMLDYDIDFSEDYGEIKKDNLYNQLNSIVVEMQQVLQTGQAGRMFT
                     QGLKIAITGKPNVG"
     gene            780..1634
                     /locus_tag="PLW07_04080"
     CDS             780..1634
                     /locus_tag="PLW07_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HOL49735.1"
                     /translation="MAAKMKSYFLLGILSVLFVYIGSLFGNEWMVFAFFMALVMNFVS
                     YFLGDKIVLAMTRAVMVPENQMPRLHRIVEEVAVSAGIPKPTIYIIPSASPNAFATGR
                     DPAHSSVAFTQGILELLDENELKGVIAHEMSHIKNRDILVATIAATIASAIMMIARMI
                     QFAAIFGGGGRDSDRGGNIFSVFAMIAFAILVPIVASLINLAISRSREYLADETGAKL
                     IHNPFALANALRKLARGVSGAPMNSVNPSISHLFVVNPFSAKSFFNLFSTHPPMEERI
                     RRLENMSF"
     gene            complement(1631..2710)
                     /locus_tag="PLW07_04085"
     CDS             complement(1631..2710)
                     /locus_tag="PLW07_04085"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOL49736.1"
                     /translation="MTSEDKLLSILNKKAPGSLSWTALVDDNTLNLLPDHLRANYGID
                     FYKHIGCDIILLNGWNTPFTFKSPQLKWSDVRQENYQENQKTVKKWFSRYGNLTGIYG
                     KGGHPVKYPVDSIEAVKIYTKMWEQACFIPHDDSQTYKILKKLVGNSGVITRFWGPST
                     IPRLLEMDMGTTNFYYLLHDYPDEMKNLINMIHNVEKQAFKILAQHPCQCIILVENTS
                     TYYISPKIYEEFNMLHQYEFVKEVKKTGKTAILHMCGHVRNLLPLIKKTECDGIHALT
                     PPPTGDTPWELALDVLGEDTIIISALDPSVFILSNIEEISVALDKIVTPRLRKANFVL
                     GVFADGISVEPERFYAVAKWVEKNS"
     gene            2819..2892
                     /locus_tag="PLW07_04090"
     tRNA            2819..2892
                     /locus_tag="PLW07_04090"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2853..2855,aa:Asp,seq:gtc)
     gene            3110..4855
                     /gene="thrS"
                     /locus_tag="PLW07_04095"
     CDS             3110..4855
                     /gene="thrS"
                     /locus_tag="PLW07_04095"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOL49737.1"
                     /translation="MSENPDNNLEALRHTASHILAQAVKRLHPDTKLCIGPAIENGFY
                     YDFFRNEPFTPQDLENITSEMKKIVKENLPVERVEYDKKKAEEILKDEPFKLEILSEI
                     QNEKVSFYKQGEFIDLCAGPHVSSTGKVKHFKLTNVSSAYWRGDEKRESLQRIYGVCF
                     PEKEQLDQYLMTVEEAKKRDHRILGPALGLFSLQPDFGAGLVYWHPAGAAVRKVIEDF
                     WRETHLERGYQLLYTPHIASISLWEKSGHLSFYRENMYPPMDMEQGISYQLKPMNCPF
                     HILVYQTTLHSYKEFPIRWAELGTVYRYEKDGVLHGVLRVRGFTQDDAHVFCRPDQIE
                     NEVVEVLDLVMFFLSKFGFTEYEIFLSTRPDKFVGSIENWEKATKSLENALKKKNLEY
                     QIDPGEGVFYGPKIDLKIRDCLKRTWQCSTIQVDFNIPERFGLKYVNQENKFERPIMI
                     HRAVMGSLERFFGILIEHYSGNFPLWLAPVQVKILTVADRFTPYGEEVTQLCRENQIR
                     AGFDGSQESLNKKIHMAENEKVPVIAIVGEKEQSTRTVAVRLHKQGMKGVFPLQDFVS
                     KLKEIDRNKFIQLDL"
     gene            4920..>5199
                     /gene="infC"
                     /locus_tag="PLW07_04100"
     CDS             4920..>5199
                     /gene="infC"
                     /locus_tag="PLW07_04100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOL49738.1"
                     /translation="MDPRRYNVNYRIRASQVRLIGEDGQQIGVVPRDQAIAMAKERLM
                     DVVELVPNANPPVCRILDFKRFLYEQKKKEKETKKKQKAVQMKEIRLRP"
BASE COUNT         1545 a          920 c         1140 g         1594 t
ORIGIN      
        1 ccaacgtttg gttttcctgt aattgcaatt ttcaatccct gggtaaacat tcttcctgcc
       61 tgtcctgttt gtaaaacctg ttgcatctcc acaacaatac tgtttaactg gttgtataaa
      121 ttgtcttttt ttatttctcc gtaatcttca gaaaaatcaa tgtcataatc caacatagta
      181 agaatattga gcaacttttt tcggaattca gagattctta cagaaagaga accagtgagt
      241 tgattgactg ctatttcaag agatttttct gtttttgcat taatgacttc aagaatgctt
      301 tctgcctgag cgagatctat tcttccattg agaaacgctc tttttgtaaa ttccccgggt
      361 tctgcaaacc gtgccccatt tcgaaggcac aatgcaataa cagacttaat agcggcaatg
      421 cctccatggc atccaatttc tgccatgtcc tctcttgtat aacttgaagg agatttcatg
      481 attgttacca gcacttcatc cacaattttt tcgccatcaa caatatgtcc gtatctaaca
      541 gtgtgtgtct cccatacaga cggaagtttt cttcttctgg caggtttgaa aatttttttc
      601 acaatttgaa atgtttcagg cccgctgagt ctgataatgc caattccgct tattccgtat
      661 ggcgttgaaa gtgcgcagat ggtatcctta tccatagtaa tttatcgtgt tatgctatac
      721 tttattatac cgcaacttct ttatgctttt gaacatctaa taaaaaacgg aggttcaata
      781 tggcggcaaa aatgaaaagt tattttctac tgggtatcct gagtgtactg tttgtttata
      841 taggaagttt gtttgggaat gagtggatgg tttttgcatt ttttatggct cttgtcatga
      901 atttcgtttc ttactttctt ggcgataaaa ttgtccttgc gatgacgagg gctgttatgg
      961 tacctgaaaa ccagatgccg cgtcttcata gaattgttga ggaagttgca gtatctgcag
     1021 gcataccaaa accgacgatt tacataatac cttctgcaag ccccaatgct tttgccactg
     1081 gaagagaccc ggctcattct tcggttgctt ttacccaggg aatacttgaa ctgcttgatg
     1141 aaaatgaatt gaaaggggtc attgcgcatg agatgtcgca tattaaaaac agggatatac
     1201 ttgttgcaac aattgcagca acaattgcaa gtgccatcat gatgattgca agaatgattc
     1261 agtttgctgc gatttttggt ggaggtggca gagattcaga tagaggtgga aatatctttt
     1321 ctgtttttgc catgattgct tttgcgattc ttgttcccat cgtggcaagt ttgattaatc
     1381 ttgcaatttc aagaagtaga gaatatcttg ccgatgaaac aggagcaaaa cttattcaca
     1441 atccttttgc acttgcgaac gcattgagaa aacttgcaag aggggtgtct ggtgcgccta
     1501 tgaacagtgt caatccatcc ataagccatc tttttgttgt gaatccgttt tctgcgaaat
     1561 ctttttttaa tttgttctcg acgcatccgc ctatggaaga aagaataaga agacttgaaa
     1621 acatgtcttt ctaactgttt ttttcaaccc attttgcaac tgcgtaaaat ctttcaggtt
     1681 ctacgctgat accatctgcg aaaacaccaa gaacaaagtt tgcctttctc agccgcggag
     1741 taacaatttt atcaagggcg acagaaattt cttcaatatt gctcaggata aaaacagaag
     1801 ggtcaagggc actgataata attgtgtctt cgccgaggac atcgagggca agttcccatg
     1861 gagtgtctcc tgttggcggg ggcgtgagag catgaatacc atcacattca gtttttttaa
     1921 tgaggggaag aagatttctt acatgcccgc acatatgcag gattgccgtt ttcccggtct
     1981 ttttcacttc ttttacaaac tcatactggt gaagcatgtt aaattcttcg tagattttcg
     2041 gactgatata ataggtgctt gtgttttcaa caagtattat gcactgacag gggtgttgag
     2101 caaggatttt gaatgcctgt ttttcaacgt tgtgtatcat attgatcagg tttttcattt
     2161 catctggata gtcatgaaga agataataaa agttggttgt acccatatcc atttcaagca
     2221 atctcgggat tgttgaaggt ccccagaacc ttgttattac tcctgagtta ccaaccagtt
     2281 ttttcaatat tttgtatgtt tgagaatcgt catgtggaat gaagcatgcc tgttcccaca
     2341 ttttcgtata gatttttact gcttcaattg aatcaacagg atacttaaca ggatgtcctc
     2401 ctttgccgta aatgcctgtt agatttccat agcggctgaa ccattttttt actgttttct
     2461 ggttctcctg ataattttcc tgtctgacgt cagaccattt cagttgtggc gatttgaatg
     2521 tgaagggtgt gttccatcca ttgagaagaa tgatgtcgca tccaatgtgt ttataaaaat
     2581 caatcccgta attagcgcga aggtggtctg gcaaaagatt taaagtgttg tcgtcaacaa
     2641 gggctgtcca ggaaagagaa ccaggcgcct ttttattaag aattgacaga agtttgtctt
     2701 ctgatgtcat ttgatttcct tgacgcttat tataatacaa aacgcaatca ggggaaatag
     2761 ttattaatag ttatttttga cattttcata ttgaaaagat tataatataa cgataagagg
     2821 ggccgtagtt cagcggatta gaatgccgga ctgtcgatcc ggaggtcgcg ggttcgaacc
     2881 ccgtcggccc cgcttaaatt tttgacttac aggggtgaga attaagcgaa gtaaggaatt
     2941 gcgagcgagc gcagcgattc cagcgagtgg ccgatcctga gtgtagtggg aacgaagggc
     3001 gccgaacccc gtcggccccg cttaaatttt taacttacag gggttgggag taataaatcg
     3061 cgaacagaaa aatcagtata actaattttt tgtgggaacg attatccata tgagtgaaaa
     3121 tcctgacaat aatcttgaag cattaaggca tacagcatca catattcttg cccaggctgt
     3181 aaaaaggctg catccggata caaaactttg tataggccct gcgattgaaa atggttttta
     3241 ttatgatttt ttcagaaatg aaccgtttac ccctcaggat ttagaaaaca tcacatctga
     3301 aatgaaaaaa attgtgaaag aaaatctacc tgttgaaagg gttgaatatg ataaaaaaaa
     3361 ggcagaagaa atccttaaag atgaaccgtt caagcttgaa attctttcag agatacagaa
     3421 cgagaaggtt tctttttata aacagggaga atttattgac ctgtgtgcgg gtcctcatgt
     3481 ttcttcaaca ggcaaggtga aacattttaa gttaaccaat gtttcttctg cttactggag
     3541 aggtgatgaa aaaagggaaa gccttcagag aatttacggt gtttgttttc ctgaaaaaga
     3601 gcaacttgac caatatttaa tgactgttga agaggcaaaa aaacgagacc acagaattct
     3661 gggtcctgcc ctgggtttgt tttctctaca acctgatttt ggggcagggc tggtttactg
     3721 gcatcctgca ggtgctgctg taaggaaagt tattgaggat ttctggagag agacccatct
     3781 cgaaagggga tatcaactat tatacacacc tcatattgca agtatttcat tgtgggaaaa
     3841 gtccggacat ctttcttttt acagagaaaa catgtatcca ccaatggata tggagcaagg
     3901 gatttcttat caattgaaac caatgaactg tccttttcat attcttgttt atcagacaac
     3961 tttgcacagt tacaaagaat ttccaatacg atgggcagaa cttgggacag tgtacaggta
     4021 tgaaaaggat ggtgttcttc acggagtact cagggtgagg ggatttaccc aggatgacgc
     4081 tcacgttttt tgcaggccag accagataga aaacgaggtt gttgaggtac ttgaccttgt
     4141 gatgtttttc ctttcaaaat ttggtttcac tgaatacgaa atttttcttt ccacacggcc
     4201 cgataaattt gttggttcta ttgagaactg ggaaaaagca acaaagtcgc ttgaaaatgc
     4261 gcttaagaaa aagaatcttg aatatcaaat agaccctgga gagggagttt tctatggtcc
     4321 gaagatagat ttgaagatac gggattgtct taaaagaacc tggcagtgtt caacaatcca
     4381 ggttgatttc aatattccag agagatttgg tttgaaatac gtgaatcagg aaaataaatt
     4441 tgaaagacct attatgatac acagagcagt gatgggttct ctggaaagat tttttggtat
     4501 actgattgaa cattacagtg gaaatttccc gttgtggctt gcaccagtgc aggtgaaaat
     4561 tttgacagtt gcagataggt ttacacccta tggagaagaa gtaacgcaat tgtgcaggga
     4621 aaatcaaata cgggcaggat ttgatgggtc tcaggaatca ttgaacaaga aaattcacat
     4681 ggcagagaat gaaaaagtgc ctgtcattgc tattgttggt gaaaaagaac aatcaacacg
     4741 cacagttgct gtaagattgc acaaacaggg gatgaaaggc gttttcccgt tacaggattt
     4801 tgtcagcaaa ttgaaggaga ttgatagaaa taaattcatc caattagacc tttgatttgt
     4861 cagcaggaga attttaagta taataatata tgatgaaaaa gtgagaaaaa ggagaaaata
     4921 tcgacccacg caggtataat gttaattata gaatacgggc atctcaggtt cgactgattg
     4981 gagaagatgg tcagcagata ggagttgtac cgagagatca ggcaatagcg atggcgaagg
     5041 aacggttgat ggatgtggta gaactggtgc caaacgcaaa tcctcctgtg tgcagaatac
     5101 tggatttcaa acgtttctta tacgaacaaa agaaaaaaga aaaggaaaca aagaagaaac
     5161 aaaaagcagt tcagatgaaa gaaatccggt tgaggcctg
//
LOCUS       DAORYU010000094         5097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_11135_length_5097_cov_5.452003, whole genome shotgun sequence.
ACCESSION   DAORYU010000094 DAORYU010000000
VERSION     DAORYU010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5097
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11135_length_5097_cov_5.452003"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..1244)
                     /locus_tag="PLW07_04105"
     CDS             complement(105..1244)
                     /locus_tag="PLW07_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49739.1"
                     /translation="MINTRQDILALLRGQSTGRIPVSLYKIDPYDEKSFWAGHTSFHR
                     LLEKMRNDCYNFFFFRPATGFFFSAPGSIEVKEDRKQDTRISEVVTLTVETEKGILTR
                     SARTTKLSAEQWVLKPWITSTHDIEKFLSLPYTPYVPDLSQFQEKQALYGNKSMPVIS
                     LPDPAGVAGTLFAPGDLGKFLLDDEKQIAHLFELFTERLANIYRTISMQVHNVIIRIR
                     GAEYLTPPSLPVEYFRNYRDVFVNYVIKPDTKLINILRSGNLNYICFHWHEMREDLLP
                     HVLKMGVDILEPAINTVEFPMTVAKVRKIIGPDITLIGGPALEEIEFRSSYEISQISK
                     DSIVQNGRSTKFILIPSGVPSSIPLSSQTEENLFAMIDAAKSFTF"
     gene            complement(1241..2083)
                     /locus_tag="PLW07_04110"
     CDS             complement(1241..2083)
                     /locus_tag="PLW07_04110"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOL49740.1"
                     /translation="MIKKSINLWVAPPDSDIYDVLSVIKDAGFDAVEPNLDEQGYMNP
                     ESTEKEIEAFRNRVEKIGLEIASVSSGLLWKYTLSSPDAQVRKKAESLIKKQIECAKI
                     LGTDGILVIPGVVQADWSGQQDIVSYDVALERVRESLNNVIDFAEKNKVCLCLEPVWN
                     KFLISPVEMKNFVESFGSDYVGVYFDTGNVMLYGYPEMWIRILGKHIRKIHFKDFKVQ
                     VGNLNGFCMLLEGDVNWQQVMKALKEINFNGYATAEFWAYKYCPETVIYHASTSLNRI
                     LKLS"
     gene            complement(2150..2734)
                     /locus_tag="PLW07_04115"
     CDS             complement(2150..2734)
                     /locus_tag="PLW07_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010479171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1614 domain-containing protein"
                     /protein_id="HOL49741.1"
                     /translation="MVVLPIQAVKIALEKIGLSPVVAFFVMLASLAGSFINIPLAVQT
                     PGGFVPAFSYFGGFLIDVPAGIKVIAINVGGAIVPLCLCLYLIPKTKFLPLFFATLIS
                     ALICHKLARVVPGIGVQLPALIPPVVAVFLAFLFSPGNKIPVAYISGVLGVLVGADLM
                     NLGNLGDFAGMMSIGGAGVYDGIFLVGIISALLA"
     gene            complement(2800..3546)
                     /locus_tag="PLW07_04120"
     CDS             complement(2800..3546)
                     /locus_tag="PLW07_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOL49742.1"
                     /translation="MKAMYQKPESLTSDVTTYCPGCGHGIIQKLIAEVIDELGVREKT
                     IGIAPVGCAVLSYNFFNFDVTEAAHGRAPAVATAIKRLFPTRIVFTYQGDGDLAAIGT
                     AEIVHAAARGENFSVVFVNNAVYGMTKGQMAPTTLIGQKTTTTTDGRTPQSHGFPIKV
                     CELLLSLDGVTYLERTTISDIPGILATKRAIKKAFLWQMKNKGMGFVEVLSTCPVNWK
                     KSPSESMKWMKETMEKYYPVKIFKDPGISI"
     gene            complement(3543..4586)
                     /gene="vorB"
                     /locus_tag="PLW07_04125"
     CDS             complement(3543..4586)
                     /gene="vorB"
                     /locus_tag="PLW07_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOL49743.1"
                     /translation="MKLELLNGCEASAEGAISAGCKFYAGYPITPQNRYPEYMSERME
                     EAGGVFIQAESETSSINMVFGAAAAGFRAMTSSSSPGISLMQEGISYLAGCELPAVII
                     NMNRGGPGLGDVTPSQSDYFQSTRGGGHGDYRTIVLAPWSCQEIHDFTYLAFDLADKY
                     RNPVVVLGDGLIAQMYETVRLKPRKKRFPKKPWALDGCSGRKPNSLKSLFLTPGVLEA
                     HNWKLAAKFAKMEKREVRYESYYCSDAEYLVISFGTCARITKEAVERARSEGMSIGFF
                     RPVTLWPFPYSQLADCMKNTKKILVVEMSMGQMIDDVKIAARKSDARIFFYGRPGGGV
                     PNPDEIYKEIKKK"
     gene            complement(4583..>5097)
                     /locus_tag="PLW07_04130"
     CDS             complement(4583..>5097)
                     /locus_tag="PLW07_04130"
                     /inference="COORDINATES: protein motif:HMM:NF018701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1318 domain-containing protein"
                     /protein_id="HOL49744.1"
                     /translation="MVTIYVTFPEEKIKKAAEDIEKMIESNMGSKAMFCKLVHFVSTS
                     AFAQESSVSSEIKTDSPAIKEAINQIKSWTDELASYKKAGYIGETKDYQVAIIKLPDD
                     EQLARKVKEIVQKENKQRNIILEEIFRLNNVAPEQKQTFRELFAKTKLKNVKPGEWYQ
                     NPDGSWAQKK"
BASE COUNT         1515 a         1128 c          920 g         1534 t
ORIGIN      
        1 cgcaaagtga aaaagaaatc aacaccatcg caacattaca aaatggaaaa actgttagaa
       61 tgaaaagcaa agaaacaaaa cttgtggcgc ttgattaatg tatgttaaaa agtgaaactt
      121 tttgctgcat ctatcatggc aaaaagattt tcttctgtct gactgcttaa tggaattgaa
      181 gaaggcaccc ctgagggaat gagaataaat tttgtgcttc ttccattttg aacaattgag
      241 tccttgctga tttgagatat ttcataagac gaacgaaatt caatttcttc aagagcaggt
      301 ccacctatca gagtaatgtc aggcccaata attttcctca ccttcgcaac agtcattgga
      361 aattcaacag tgttgatggc aggttcaaga atgtcgacac ccattttcaa cacatggggt
      421 aaaaggtctt cacgcatctc atgccagtga aaacagatat aattcaaatt cccagaacga
      481 agaatattga taagctttgt atcgggcttt atcacataat taacaaacac atcgcgatag
      541 ttccgaaaat attcaacggg tagtgatgga ggcgtcaggt actctgctcc acgtatcctt
      601 ataattacat tgtgaacctg cattgatatc gttctgtaaa tatttgccag tcgttcggta
      661 aaaagttcaa aaagatgcgc gatctgtttt tcatcgtcaa gcagaaattt tccaagatct
      721 cctggcgcaa atagtgtccc tgcaacacct gcagggtctg gaagggaaat gacaggcatt
      781 gatttgtttc cataaagtgc ctgcttctcc tgaaactggc ttaagtcagg aacataggga
      841 gtatagggta atgacaggaa tttttctatg tcatgcgtgc ttgttatcca tggtttcaac
      901 acccattgtt ctgcgctcaa ttttgtcgtt cttgcagaac gggtgagaat ccccttctct
      961 gtctcaacgg tgagcgtaac cacttctgaa atccttgtgt cctgttttct gtcttctttc
     1021 acttcaatgg agccaggagc agaaaagaaa aagccagtgg caggcctaaa aaagaaaaaa
     1081 ttgtagcaat catttcgcat cttttcaaga agccggtgaa aactggtgtg tcctgcccag
     1141 aaactctttt cgtcataagg gtcaatttta tagagagaaa ctggaattct acctgtgctt
     1201 tgacctcgca gtaaggcaag aatgtcctgc cttgtgttga tcatgacagt ttcagtatcc
     1261 tgttaagact ggttgaagcg tgataaatca ctgtttcagg acagtatttg tatgcccaga
     1321 attctgctgt tgcgtatcca ttaaagttga tttctttgag tgccttcatt acctgttgcc
     1381 agttcacatc tccttcaagc aacatacaga acccattgag atttccaacc tgaactttaa
     1441 agtccttgaa atgaattttc ctgatatgtt ttccaagaat ccttatccac atttcaggat
     1501 atccgtaaag cattacattg cctgtgtcaa aataaacccc aacataatca gacccaaaag
     1561 attcaacaaa atttttcatc tccacagggc taattaaaaa tttgttccag acaggttcca
     1621 gacacaggca tactttattt ttttcagcaa aatcaatcac attgttcaat gattctctga
     1681 ccctctccaa tgcaacatca tatgacacta tgtcttgctg accagaccag tcagcctgaa
     1741 caacaccagg tatcacaagg ataccatctg ttccaagaat ttttgcgcat tcaatctgtt
     1801 tttttatcaa agattcagcc tttttgcgga cctgtgcatc aggactgctt aacgtgtact
     1861 tccacagaag tccagaactg acactggcta tttcaagccc aattttttca actcgattcc
     1921 tgaaggcttc tatctctttt tctgtacttt ctggattcat atacccctgt tcatcaagat
     1981 tcggttctac tgcatcaaaa ccagcatcct tgataacaga aaggacatca taaatatctg
     2041 aatctggtgg tgcaacccat aagttaatac tttttttaat catcattcct ccgtggtttt
     2101 ctcacatatc attttatgga attccataaa tctgtcaata ccaggaactt tatgcaagga
     2161 gtgctgaaat aattccaaca agaaaaatac catcataaac acctgctcca ccgatactca
     2221 tcatcccggc aaaatctcca agatttccaa gattcatcaa atcagcacca acaagaacac
     2281 caagaacccc actgatgtat gccacaggga ttttattgcc tggagaaaat aaaaacgcaa
     2341 gaaatactgc aacaacagga ggaatcagtg caggtaattg tactccgatg ccaggaacca
     2401 ctcttgcaag tttatgacat atcaatgcag agataagtgt ggcaaaaaaa agtggaagaa
     2461 attttgtctt cggaataaga tacagacaga gacacaatgg cacaattgcg ccacccacat
     2521 tgattgcgat taccttgata cctgcgggaa catctattaa aaatccacca aaataagaaa
     2581 acgcaggaac gaaaccacct ggtgtttgca cagcaagagg aatattaatg aaactgccag
     2641 caagggacgc cagcattaca aaaaatgcaa caacagggga caaccctatt ttctcaaggg
     2701 caatttttac tgcctgtatt ggaagaacaa ccaaaaaaag gaacaacaga atcaaaaaga
     2761 aaaaaaacaa aaacattatt gatagtggga caaaaaatat catatactta ttccaggatc
     2821 tttaaaaatt tttacagggt agtatttttc cattgtctcc ttcatccact tcatggattc
     2881 tgaaggactt ttcttccagt ttacggggca ggttgaaaga acttcaacaa aacccatacc
     2941 cttatttttc atctgccata agaacgcttt ttttatcgct ctttttgtag caagaattcc
     3001 gggtatatca gaaattgttg ttctttcaag gtatgtaaca ccatcaaggg aaagtaaaag
     3061 ttcacatact ttaataggaa aaccatggga ttggggggtt ctaccatcag ttgttgttgt
     3121 ggtcttttga ccaatcaggg ttgttggagc catctgtcct ttcgtcattc catagacagc
     3181 attattgacg aaaaccacag aaaaattttc gcctctggca gcagcatgaa caatctctgc
     3241 agttccaata gcagcaagat ctccatcccc ctgataggta aacacgattc ttgtcggaaa
     3301 taatcttttg attgcagttg caacagcagg tgctcttcca tgcgctgcct ctgtgacatc
     3361 aaaattaaaa aaattataag aaaggacagc acagcccaca ggagcaatac caatggtttt
     3421 ttctctaaca ccaagttcat caatcacttc tgcaatcagt ttttgaataa tgccgtgtcc
     3481 gcaaccagga caataggttg taacatcaga tgtcaaactt tctggtttct ggtacatggc
     3541 tttcattttt tctttatctc cttataaatt tcatcaggat ttggaactcc gccccctggc
     3601 cttccataga aaaagattct cgcatctgat tttctggctg cgatttttac atcatctatc
     3661 atctgaccca tactcatttc aacaacaaga attttttttg tatttttcat gcaatcagca
     3721 agttgtgagt atggaaatgg ccagagtgtc acgggtctga aaaaaccaat gctcattcct
     3781 tcacttcttg ctctttcaac tgcctctttt gtaattctcg cgcatgtgcc aaaacttatg
     3841 acgagatatt ctgcatcgct gcaataatag gactcatacc gtacttctcg tttttccatt
     3901 ttcgcaaact ttgctgcaag tttccagtta tgggcttcaa gaacacccgg agtgagaaaa
     3961 agcgatttca gtgagtttgg ctttcttcca gagcatccat caagagccca cggctttttt
     4021 ggaaatcttt tctttcttgg ttttaatctc actgtttcat acatctgggc aatcagcccg
     4081 tctccgagaa caacaacagg atttctgtat ttatcagcaa ggtcaaaagc aagataggta
     4141 aaatcgtgta tttcctgaca tgaccatgga gcaagaacaa tagtacgata atctccgtgt
     4201 ccaccaccac gggttgattg aaagtaatct gactgacttg gtgtcacatc tccaagacct
     4261 ggacctcctc tgttcatatt gataatgact gctggaagtt cacatcctgc aagataggaa
     4321 atcccttcct gcatcaacga tattcctgga cttgaagacg atgtcattgc cctgaatcct
     4381 gctgcagccg caccaaatac catattgatt gaggatgttt cactctctgc ctgaataaat
     4441 accccgcctg cctcctccat cctctcgctc atgtattccg ggtatctatt ttgaggagta
     4501 atgggataac ctgcgtaaaa tttgcaacca gcagatatcg cgccctcagc cgaagcttcg
     4561 caaccattga gaagttctaa tttcattttt tctgtgccca ggaaccatca gggttttgat
     4621 accactctcc gggtttcaca tttttcaatt ttgttttcgc aaaaagttct ctgaatgtct
     4681 gtttctgttc aggtgcgaca ttattgagcc ggaaaatttc ctcaagaata atgtttctct
     4741 gtttgttttc tttttgaaca atttccttta cttttctcgc aagttgctca tcatcaggca
     4801 acttaataat tgccacctga tagtcctttg tctcaccgat atatcctgcc tttttgtacg
     4861 atgcaagttc atctgtccac gatttgatct gattgatggc ttccttgatc gcaggagaat
     4921 ctgttttgat ttctgaactt acggatgact cctgggcaaa agcagacgtg gaaacaaaat
     4981 gaactaattt gcaaaacatt gccttgctgc ccatgtttga ttcaatcatt ttttctatgt
     5041 cctcagcggc tttcttaatc ttttcttcag gaaaggtcac atagatcgtc accattg
//
LOCUS       DAORYU010000095         5058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_11248_length_5058_cov_4.620028, whole genome shotgun sequence.
ACCESSION   DAORYU010000095 DAORYU010000000
VERSION     DAORYU010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5058
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11248_length_5058_cov_4.620028"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1325)
                     /locus_tag="PLW07_04135"
     CDS             complement(<1..1325)
                     /locus_tag="PLW07_04135"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HOL49745.1"
                     /translation="MMSRKWIAAVILLTFGIIGFLYSINAFNRFTHLAYDLLIRSVFP
                     ESAKNVDVVLVTATENFVSEIGHEPNRSDYAKLLYLLGESELVVSDIFFPSLQSEEMD
                     NNLRKALVACSDKIILPVFTPYKLDKNRAFETGYVVSTLNENHPYFQKAVKYIGHINV
                     FPDSDTIVRKCPAFIFFKGQSYPHVVIKALSIHKKNGHINVSRFSGFRKLQDTIPVDR
                     DGCFAIRYVSPSNLVNAIYTMEDVLKGKVSPETFKGKIVIIGHTIMGSKNADLIPTPL
                     GVQFGALVQMQVLYTVFSGNYIWTIDQKIVLLFTFFIIFIMMFAFFSSFWRGTRSFVF
                     CVLALLYSVFILFKNNVFFDPVPGLFASVFAYASFVVMNFFEARKEISKGHQLLSVLE
                     TTQREIASIVKPQEMPGMQKRVYLPTIHDTFFYRTPAITLKTINSLLGI"
     gene            complement(1337..2260)
                     /locus_tag="PLW07_04140"
     CDS             complement(1337..2260)
                     /locus_tag="PLW07_04140"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOL49746.1"
                     /translation="MKKGFSLIEGIIVSFIVVSLAAMVLGLISRSRTLSRSMVCLNNL
                     RQISMAIENYQADWKGSPNQLYNLFPNYIKDKYSFKCPEDKDVSTGDLPNKNSYGNFY
                     VNRSFNDEDTGKLYLFCPRHFNGTKGVGAFLSYAANIMTNNPVTWNGTRMMPGQINQG
                     GTYKFADGTTVTADPSLKVGLCGSFVSPDDKHYSIIFVPEDSSGSLTVDHQGDSRFEV
                     VTPALIAGVSGTKFTVNSAYDSATNQATTTVAVSQGNVQCEDRTDGTKNIVKTTERIG
                     ITVVCQKPDSSSTQKTRSVPVRPLKKIKVVR"
     gene            complement(2257..2832)
                     /locus_tag="PLW07_04145"
     CDS             complement(2257..2832)
                     /locus_tag="PLW07_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49747.1"
                     /translation="MGRRRILVDREIQFRSVLYVLLFMVLTSIFVSLATFNSVWSLLV
                     NYLAAANEKNLTHIFDVTFRTFAVRITLLTIFLAVLATLGMLLVSHRIAGPVFRIRQI
                     LKELQEGKSPVFTLRKGDSLSALMDEIRNFGLTYIHLVQASKRVLEIWNKTQVQDMSL
                     NLALKDLEECFQEKIKEENLTVEKKLKEEGT"
     gene            complement(2889..3206)
                     /gene="trxA"
                     /locus_tag="PLW07_04150"
     CDS             complement(2889..3206)
                     /gene="trxA"
                     /locus_tag="PLW07_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOL49748.1"
                     /translation="MKVLNDENFDAVLKENSVVLVDFYADWCGPCRMMAPVVEEIATE
                     LEGKMVVAKLNVDENPDIAARFQIFSIPTFILFKNGKAEEKIIGAVGKAGLLEKIKSY
                     LGS"
     gene            complement(3259..4254)
                     /locus_tag="PLW07_04155"
     CDS             complement(3259..4254)
                     /locus_tag="PLW07_04155"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOL49749.1"
                     /translation="MKTFHVQWHITNTCNLRCKHCYHDSYRVSDDLRFPQIQVLFDGL
                     VKFLKKNKYHLTVDITGGEPFFHPDFWQIIQMLENCNHVMQCGIITNGTVLTEKILNQ
                     LAGFSKIKVVKISCEGSDKQSFEKIRPFPYEKFLKILELVSMFKFEKLLMFTLLETNS
                     DQISGLFNLVTRFNLDGFIMERFFPMGAGKKLNNFFVTNETWQSTVKQLLTSCGMPDD
                     LMLVAEYRGFKVLRKKNRWELFGAPCIVGKYGCAIMHDGNVFPCRRFVLSIGNALIEP
                     FEKIWMKNPCRTMRRKNLKGNCGVCSIKKCYGCRALAHCIYGDCFAEDPLCFLNV"
     gene            4348..>5058
                     /locus_tag="PLW07_04160"
     CDS             4348..>5058
                     /locus_tag="PLW07_04160"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOL49750.1"
                     /translation="MKRYVLGLDYGTNSMRAVVVNLLTAREVIELVENYPSGKDGVIL
                     DHKNAHLARQNPADYLYCLEKIGKNLTKLMKRKGIKSEDVVGIGIDTTGSTPIPVDEN
                     LVPLVMKREFRNNKNAMAWLWKDHTSADEAEEITNLAKKIRPEYLSKIGGVYSSEWFF
                     SKILHLARTDKKVFEASASFVELCDFIPAILTGCTSPSQVLRSICAAGHKAMYNPQWG
                     GLPDEKFLEMLDPSFKNLR"
BASE COUNT         1612 a         1049 c          886 g         1511 t
ORIGIN      
        1 aaaatcccga gcaaagaatt gattgttttc aatgtaatgg caggcgttct gtagaaaaat
       61 gtatcgtgta ttgtgggcaa ataaacccgt ttctgcattc ctggcatttc ctgaggtttg
      121 acaattgatg caatctccct ctgggttgtt tcaagaacag aaagaagctg gtgccctttt
      181 gaaatttcct ttcttgcttc aaagaaattc atcacgacaa aactggcata agcaaaaacc
      241 gatgcgaaaa gccctggcac tggatcaaaa aacacattgt tcttaaacag aataaataca
      301 ctgtacaata acgccagaac acagaaaaca aaactccttg taccgcgcca gaaggaagaa
      361 aaaaatgcaa acatcataat aaaaataatg aagaatgtga acagaagaac tattttctgg
      421 tctatggtcc agatgtaatt tccactgaaa acagtataca gtacctgcat ttgaacaagg
      481 gcgccaaact gaaccccaag tggtgttgga atcaaatcag cattcttgga gcccataatg
      541 gtatggccaa taatcacaat tttgcccttg aatgtttctg gagaaacctt gcctttcaga
      601 acatcttcca ttgtataaat cgcattgaca agatttgaag gcgaaacata cctgattgca
      661 aaacaaccat ccctatcaac aggtattgta tcctgtaact tcctgaaccc ggaaaatctt
      721 gacacattaa tgtgcccatt ttttttatga atgctcaacg cttttataac aacatgtgga
      781 taagattgcc ctttgaaaaa aataaatgca ggacacttac gcacaatcgt atcagaatct
      841 ggaaaaacat tgatgtgacc gatgtatttt actgctttct gaaagtaggg atgattttcg
      901 ttcaatgtac ttacaacata tcctgtctca aatgccctgt tcttatcaag tttgtaagga
      961 gtaaaaacag gcaaaataat tttgtcagag caagcaacaa gcgcttttct gagattgtta
     1021 tccatttctt ctgattgcag ggatggaaag aaaatatcag ataccacaag ttctgattcg
     1081 ccaagcaagt atagcaattt cgcataatca gaccggttgg gttcatgacc gatctcagaa
     1141 acaaagtttt ctgtggcagt aacaagaacg acatcaacat tttttgctga ttctggaaaa
     1201 actgatctga ttaacaaatc ataggcaagg tgtgtaaaac gattgaaggc attgattgag
     1261 tataaaaaac cgataattcc aaacgtcaga agtattactg cagcaatcca ttttctggac
     1321 atcattcagc cacttattat cttacaacct ttattttctt aagtggcctg acaggcacgc
     1381 ttcttgtttt ttgagtactg gaactgtctg gtttctggca aacaacagta ataccaattc
     1441 tctcagtagt tttcacaatg ttttttgtac catctgtcct gtcttcgcac tgcacatttc
     1501 cctgagacac tgcaacagtg gttgtcgcct gatttgttgc gctatcgtaa gcactgttga
     1561 cagtaaattt tgttccactg acaccagcaa tgagtgcagg tgtaacaacc tcaaaccttg
     1621 aatccccctg atggtcaact gttaatgaac cactggaatc ttcaggcaca aaaatgatac
     1681 tgtaatgttt atcgtcagga ctgacaaaag aaccacaaag accaactttt aatgatgggt
     1741 ctgctgttac tgttgttcca tctgcaaact tatatgtacc gccctgattg atctgtcctg
     1801 gcatcatcct ggtaccattc caggtcacag ggttatttgt cataatgttt gcagcatatg
     1861 atagaaaggc accaactccc tttgtcccgt tgaaatgcct cggacagaaa agataaagtt
     1921 tgcctgtgtc ttcgtcatta aacgaacggt ttacataaaa attcccataa ctatttttgt
     1981 ttggaaggtc tccagtacta acatctttat cctcaggaca tttgaaagaa tacttatctt
     2041 ttatataatt gggaaataga ttataaagct gattgggact gcctttccag tctgcctgat
     2101 aattctcaat cgccatggaa atctgacgaa ggttattaag gcaaaccatt gaccttgaaa
     2161 gagtccttga ccttgaaata agtcccaata ccatcgcagc gagagacaca acgatgaatg
     2221 aaacaataat accctcaata agactgaatc cttttttcat gttccctcct cttttaattt
     2281 tttttcaact gtaaggtttt cttcctttat tttttcctgg aaacactctt ctaaatcttt
     2341 gagtgccaga ttcaaactca tgtcctgaac ctgggtctta ttccatattt caagaaccct
     2401 tttacttgcc tgaacaagat ggatgtatgt aagaccaaaa tttcttattt catccatcaa
     2461 cgcgctgagg ctatctcctt ttctaagtgt aaacactgga ctttttcctt cctgaagttc
     2521 ttttaaaatc tgccgtatgc gaaatactgg ccccgctata cggtgagaaa caagaagcat
     2581 gccaagcgtg gcaagaaccg caaggaatat ggtgagaagt gtaatacgaa cggcaaatgt
     2641 tctaaatgta acatcaaaga tatgagtgag atttttttca ttggcagcag ccagataatt
     2701 gacaagcaaa gaccagacgc tgttaaatgt tgcaagactg acaaaaatac tggtcaatac
     2761 catgaacaat agaacataaa gcacagatct gaattgtatt tcacggtcta ccagtattct
     2821 tcttcttccc atatgtctcc aggaatttta tatcttcaag gtcattatac ctgacatatt
     2881 tttacctgtc aactaccaag ataggacttg attttttcca gcaatcctgc cttgccgaca
     2941 gcaccaataa tcttttcttc cgccttgcca tttttgaaaa gaataaaggt cgggattgaa
     3001 aaaatttgaa atcttgcagc aatgtcaggg ttttcatcta cattgagttt tgcaacaacc
     3061 attttgcctt ccagttctgt cgcaatttct tcaaccactg gtgccatcat cctgcatgga
     3121 ccacaccagt cagcataaaa atccacaaga accacagaat tttctttcag cacagcatca
     3181 aaattttcat catttaatac tttcattttt cctccatgta aggttttatg ttttagatgc
     3241 catcatcgat aaaagggttc aaacattcaa aaagcataaa gggtcttcgg caaaacaatc
     3301 accgtaaata caatgcgcaa gtgccctgca accataacat tttttgatgc tacaaacacc
     3361 gcagttgcct ttcaaatttt ttcttctcat ggttctgcat ggatttttca tccagatttt
     3421 ttcaaacggc tcaattaagg cattcccgat acttaaaaca aatcttctac agggaaacac
     3481 attcccgtca tgcataatgg cgcaaccata cttaccaaca atacacggtg caccaaacag
     3541 ttcccatctg ttcttttttc tcaatacctt gaaaccacga tactctgcga caagcattaa
     3601 gtcatccggc attccgcatg aagtaagaag ttgtttaaca gttgattgcc atgtctcatt
     3661 tgtgacaaaa aaattattca gttttttgcc tgctcccatc gggaaaaatc tctccatgat
     3721 aaatccatca agattgaaac gcgtaacaag attgaataac cctgaaatct ggtctgaatt
     3781 tgtttcaagt agcgtaaaca tcagaagttt ttcaaattta aacatagaaa ccagttcaag
     3841 aatcttcaaa aatttttcat aaggaaacgg tctaattttt tcaaaacttt gtttatcgct
     3901 cccttcacat gaaattttca ctactttgat ttttgaaaaa ccagccagtt gattgagtat
     3961 tttctctgtc agaactgttc cattggtaat gatgccacac tgcatcacat gattgcagtt
     4021 ttcaagcatc tgaataattt gccagaaatc aggatggaaa aatggctcac caccagtgat
     4081 atcaaccgtc agatgatatt tatttttctt caaaaacttc acaagcccat caaaaagaac
     4141 ctgtatttga ggaaatctca ggtcatctga taccctgtaa gaatcatgat aacagtgttt
     4201 gcatcttaaa ttacaggtgt ttgtaatgtg ccactggaca tgaaatgttt tcatcaataa
     4261 attctacatc ataatagaaa ttttgcaatc tttttgattt tactttttgc gctgcctttg
     4321 ttaaaataac ggcaaaaagg agtaaccatg aagagatatg tactcggtct tgattatgga
     4381 acaaattcca tgcgagcggt cgttgtcaat ctgcttacag ccagggaggt cattgaactg
     4441 gttgaaaatt atccctcagg taaagacgga gtgattcttg accataaaaa tgcccatctt
     4501 gcccgacaga atcctgcgga ttatttgtat tgtcttgaaa aaataggaaa aaacctcacg
     4561 aaactgatga aaagaaaggg tatcaaaagt gaagatgtgg ttggtattgg tattgatacc
     4621 actggaagca ctcccatacc ggttgacgaa aatcttgttc ctcttgtaat gaaaagagaa
     4681 ttcagaaaca acaaaaacgc aatggcatgg ctatggaaag accatacttc tgcagatgaa
     4741 gcagaggaaa tcacaaatct tgcaaaaaaa ataaggccag aatacctttc aaaaattggc
     4801 ggcgtatatt cctcagaatg gtttttctca aaaattctac accttgcaag aacagataaa
     4861 aaggtttttg aggcaagcgc aagtttcgta gaactatgcg attttatacc cgctatactt
     4921 actggatgca ccagcccttc ccaggtttta agaagcattt gcgctgctgg acataaagcc
     4981 atgtataacc cgcagtgggg tggacttcct gatgaaaaat tccttgaaat gcttgaccct
     5041 tcattcaaaa atttaaga
//
LOCUS       DAORYU010000096         5011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_11397_length_5011_cov_5.767353, whole genome shotgun sequence.
ACCESSION   DAORYU010000096 DAORYU010000000
VERSION     DAORYU010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5011
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11397_length_5011_cov_5.767353"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..1964
                     /locus_tag="PLW07_04165"
     CDS             195..1964
                     /locus_tag="PLW07_04165"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013411266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HOL49751.1"
                     /translation="MSTPLEKWVQTQAELTKPDRIHWCDGSEEEARWLMEMGMEEEKI
                     NGNPVFYLLNHEKWPNAYLHRSHPTDVARTEHLTYVCHPDKQLAGPNNNWMHPDEAKQ
                     MLNRLSDGCMKGKTMYVLPYTMGHPDSPFAKACVQLTDISYVAVSMRIMTRMSKHVID
                     KIGNSENFVKGLHSVGDFDPNKRFIMHFPDEHLVWSIGSGYGGNALLGKKCFSLRIAS
                     WLGYNEGWLAEHMVIMGIEDPDGNITYVTAAMPSACGKTNLAMMQSALPGYKIWTLGD
                     DIAWFHIGDDGRLYAINPEIGFFGVAPGTSNKTNPNMMLTLKGNKFYPTLFTNTGLNL
                     DTKEPWWEGMDALPGKNMQDWQGNKWDASSNTPAAHPNSRFTVSIYNTPTLSKEFDNP
                     KGVPISAIIFGGRRTHLIPLVTEAFSWDHGVFIGARSGSETTAAAIHKVGMLRRDPMA
                     MLPFCGYNMADYFSHWMNMGKKMKHPPKIFSVNWFRKDDDGNFIWPGFGENIRVLKWI
                     IDRVNNKISAKETPIGLVPYAQDIDTTGIDLSREKLDKILDVNIKEWKEELEDVKKFF
                     EGFGSKLPGYLWEEFEKIYKQAT"
     gene            complement(1971..2426)
                     /locus_tag="PLW07_04170"
     CDS             complement(1971..2426)
                     /locus_tag="PLW07_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49752.1"
                     /translation="MKFRILASFMLLPFVSLVFAGIHPTTKEEVVSSFKTIDQLIDEG
                     KENVLHVYAAALEIQKRAVTQYQADSIAKKIISTSRISEKDFEQLREHYNFAEISIVW
                     AVSQISKVPLKQLFSEVENASIEKIMDKYGAECEYIISEIGKLNTAINK"
     gene            complement(2458..2937)
                     /locus_tag="PLW07_04175"
     CDS             complement(2458..2937)
                     /locus_tag="PLW07_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOL49753.1"
                     /translation="MERKGIKIIKADINGVVKELNKALSDEWLAYYQYWLGEKVVKGK
                     FREEVAAELGQHAADELKHAGMIADRIIQLGGTPVLKPEDWYKLTNCGYDAPENPDVI
                     PVLKQNIKGEQCAIEVYQKLAEMLKDKDPITYLMVLSILKDEIEHEDDLMNILEDIQ"
     gene            complement(3331..3510)
                     /locus_tag="PLW07_04180"
     CDS             complement(3331..3510)
                     /locus_tag="PLW07_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49754.1"
                     /translation="MKRASTVGASHSAGFGQSTAIDMADRYCFPVCFVAAPSLIVHNA
                     SVFPAGSGKTRKQKN"
     gene            complement(3584..4072)
                     /locus_tag="PLW07_04185"
     CDS             complement(3584..4072)
                     /locus_tag="PLW07_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOL49755.1"
                     /translation="MEKNLQKAMNEQIRNELYSAYLYLSMAAYCESKNLSGIAHWLKV
                     QAKEEVSHAMKFFEFMVDVGAEITLLAIDQPPTDFASVTEIFEKTLEHEKKVTAMINN
                     LVSLAQKAGDNPAMIMLQWFVNEQVEEEKNASQILETLKMIKEQGPAIVMLDRELGKR
                     EG"
     gene            complement(4085..>5011)
                     /locus_tag="PLW07_04190"
     CDS             complement(4085..>5011)
                     /locus_tag="PLW07_04190"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="catalyzes the interconversion of 3-phosphoglycerate
                     and 2-phosphoglycerate; this enzyme does not require the
                     cofactor 2,3-bisphosphoglycerate as a phosphate donor;
                     BPG-independent PGAM; aPGAM; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HOL49756.1"
                     /translation="IGMEMEKNDLAIRGNFATIDDNHTVTDRRAGRIPTEENRNLVAR
                     IQESIKSINNVQIIIQTVKEHRLAILLRGENLSPFVSENDPQKEGLPIKKITPLKEEA
                     RFTAEVLNQFLEKVEHLLAGVDTKAKSILLRGFSGIPEIEPFQQRYLLKPACIASYPM
                     YKGLAKLVGMDVVKGLESIDDEIKTLNDLYSEYDFFYLHYKKTDSSGEDGNFEKKVAA
                     IEEFDEKISTIKHLKFEVVCVTGDHSMPCVLKGHSWHPVPLAIVSKNAMPDDVDTFTE
                     RACAKGILGTIPATNIMYMLLANALKFDKFGA"
BASE COUNT         1454 a         1037 c          932 g         1588 t
ORIGIN      
        1 tgacgccagt ggaatttgaa caaaaagaga taaaacaatc acctttaacc aggaccctaa
       61 attctccctt attttgctct tgacaaatgg ggggcagtac aaaaaccctc ctgattttgc
      121 gttgtgtatt ttgtaagtta taatatattt tcaataattt aaaccccctt aacaattttg
      181 caagggagta tcttatgtca acgccgcttg aaaaatgggt acaaacgcag gcagaactga
      241 caaagcccga tagaattcac tggtgcgatg gttcagagga agaagcaaga tggttgatgg
      301 aaatgggtat ggaagaagaa aaaatcaatg gaaatccagt attttactta ttaaatcatg
      361 aaaaatggcc caatgcgtat cttcacagaa gccacccaac tgatgttgca agaacagaac
      421 atctaaccta tgtatgccat cctgacaaac aacttgcagg tccaaacaat aactggatgc
      481 acccggatga agcaaaacaa atgctaaatc gtctttcaga tggttgtatg aaaggaaaaa
      541 caatgtatgt gttaccttac acgatgggtc atcctgacag tcctttcgca aaagcatgtg
      601 tgcaacttac agatatttct tatgttgctg tcagtatgcg cattatgaca agaatgagta
      661 aacatgtgat agacaaaata ggcaacagtg aaaattttgt gaaaggactt cattctgtcg
      721 gagattttga cccgaacaaa agattcatca tgcattttcc tgacgaacat cttgtgtgga
      781 gcattggttc tggttatgga ggaaatgccc tgttaggtaa aaaatgtttt tctttaagaa
      841 tcgcatcatg gttaggatat aatgaagggt ggctcgcaga gcatatggtg attatgggca
      901 ttgaagaccc tgacggcaat attacctatg tcactgctgc aatgccttct gcatgcggaa
      961 aaacaaacct tgcgatgatg cagtcggcac tgcctggtta taaaatatgg actttaggcg
     1021 atgatattgc ctggtttcac ataggcgatg acggaaggtt atacgctatc aatcctgaga
     1081 ttgggttttt tggagtcgct ccaggaacat caaataaaac caatcctaac atgatgctaa
     1141 cattgaaagg taataaattt tatcccactc tttttactaa tactgggttg aatctggata
     1201 caaaagaacc atggtgggaa ggaatggatg cccttccagg gaaaaatatg caggactggc
     1261 aggggaataa gtgggatgct tcctcaaata caccagcagc acatcctaac tcaagattta
     1321 ctgtttccat ttacaatact cccacacttt caaaagagtt tgataatcca aagggcgtac
     1381 cgatttcagc catcatcttt ggtgggagaa gaacacactt aattccactt gtcacagaag
     1441 cattttcatg ggaccatggt gtcttcattg gagcaagaag tggttctgaa accactgctg
     1501 cagcaataca taaagtcggc atgttgagaa gagaccctat ggcaatgctt cctttctgtg
     1561 gatataatat ggcggattat ttcagccatt ggatgaatat gggcaaaaaa atgaaacatc
     1621 cgccaaaaat tttttcggtt aactggttta gaaaagatga tgatggaaac tttatctggc
     1681 caggttttgg tgaaaatatc agggtgctca agtggattat tgacagggtg aacaataaga
     1741 tttctgcaaa agaaacacct attggtcttg tgccatatgc acaggacata gataccactg
     1801 gtattgatct ttccagagaa aaacttgata aaatccttga tgttaatatc aaggaatgga
     1861 aagaagaact tgaagatgta aaaaagtttt ttgaaggttt tggaagtaaa cttccagggt
     1921 atctatggga agaattcgaa aaaatttata aacaggcaac ctgattagat ttacttattt
     1981 attgcggtat tgagtttacc aatttctgaa attatatatt cgcattctgc gccatatttg
     2041 tccattattt tttcaatact cgcattttct acttcagaaa aaagttgttt tagaggaact
     2101 ttactgattt gcgaaacagc ccatacaatt gaaatttcag caaaattata gtgttctctc
     2161 aactgctcga aatctttttc agatattctg gatgtagaga tgattttttt cgcaatagaa
     2221 tctgcctgat attgggttac tgctcgtttt tgaatttcaa gcgcagcagc atacacatgc
     2281 aacacatttt ctttgccttc gtctatcagt tggtcaatag ttttgaaact tgaaacaacc
     2341 tcctcttttg tggtgggatg tatcccggca aataccagag aaacaaaagg caataacata
     2401 aaacttgcaa gaatcctgaa tttcattgac cctctctatg aaatataaaa aacccgttta
     2461 ttgaatatcc tcaaggatgt tcatcaaatc atcctcatgt tctatttcat ccttcaaaat
     2521 tgaaagtacc attaagtaag taatcgggtc tttgtccttc agcatctcag caagtttctg
     2581 atacacttct atggcacact gttcaccctt aatattctgt ttcaacacag gtatcacatc
     2641 agggttttct ggcgcatcat aaccgcaatt tgtaagttta taccagtcct ctggcttcag
     2701 gacaggcgta ccacccagtt gtataattct gtcagcaatc attccagcat gctttaattc
     2761 atcggctgcg tgctgaccaa gttcagcagc aacttcttcc ctgaattttc cttttaccac
     2821 tttttctcca agccagtact gataataggc aagccattca tcagaaagcg ccttattcaa
     2881 ttccttcaca actccattaa tatctgcttt gataattttt attcctttcc tttccactgt
     2941 atcctccctg tcggttaatt tatttatatt tagattacca tttatcctgg caagtttcaa
     3001 ctacggcaga ttcctgcgta gttttgtctg caccgtagcg tatgtgaata atattttgct
     3061 gtcatccatt gggtattttc ctattttttt ggttttcttc tttccccact tcccgcagga
     3121 aagacagaag cattatgaac aattagcgaa ggcgcagcaa caaaacaagg ataaaagcat
     3181 ctgcgttccg cgataacctt acacctgctt gttcgaagcc ttcgcagtgc aaggcgtata
     3241 cggagaaggc aagtttgcag gtgcagcgga tttatccgta gttttgtctg caccgcagcg
     3301 tatgtgaaat aatgctttgc tgtcatccat tcagtttttt tgttttcttg ttttcccact
     3361 tccagcagga aagacagaag cattatgaac aattagcgaa ggtgcagcaa caaaacaaac
     3421 aggaaagcaa taacggtctg ccatatcaat agcagttgac tgtccgaagc cagcagagtg
     3481 cgaagcgccc acggtgctgg cacgtttcaa ctgcggcaga ttcctgcgta gttttgtctg
     3541 caccgcagcg tatgtgaaat aatgcttctg tctttcctac ccctcatcct tctctttttc
     3601 ccagctccct atccagcatc acaatcgcag gtccctgctc ttttatcatt ttcaatgtct
     3661 caagaatctg tgatgcattt ttctcttctt caacctgctc attcacaaac cattgaagca
     3721 taatcattgc agggttatct cctgcttttt gagcaagaga aacaaggtta ttaatcattg
     3781 ctgtcacctt tttctcatgc tcaagggttt tttcaaatat ctcagtgaca gaagcaaaat
     3841 ctgttggtgg ctggtcaatg gcaagaagtg ttatctctgc tcccacatca accataaact
     3901 caaaaaattt catcgcatgg cttacttctt cttttgcctg aactttcaac cagtgagcaa
     3961 ttcctgaaag gtttttgctt tcacaatacg ctgccatgct taaataaaga taggcagaat
     4021 ataattcatt ccttatctgc tcattcattg ccttctgcaa attcttttcc atcttcctcc
     4081 cttgttatgc tccaaatttg tcaaatttta aggcgttcgc aagcaacata tacatgatat
     4141 ttgttgcggg tattgttcca agtattcctt ttgcacatgc cctttctgta aatgtatcaa
     4201 catcatctgg cattgcattt tttgaaacaa ttgcaagtgg tacaggatgc caggagtgtc
     4261 cttttaaaac acatggcata gaatggtctc ctgttacaca taccacttca aattttagat
     4321 gcttgattgt tgaaattttt tcatcaaact cttctatggc tgcaaccttt ttctcaaaat
     4381 ttccgtcttc tcctgaactg tcggtcttct tgtaatggag atagaaaaag tcatattcac
     4441 tgtagaggtc gttgagtgtc tttatttcgt catcaatact ttcaagtccc ttaaccacat
     4501 ccatcccgac aagttttgca agtcctttgt acataggata acttgcaata cacgctggtt
     4561 ttaaaagata tctctgttga aatggctcta tctcaggaat acctgaaaat ccccttaaca
     4621 gaatgctttt tgcttttgta tctacacccg ccagaaggtg ttcaaccttt tcaagaaact
     4681 gattcaaaac ttctgctgta aatctggctt cctctttcaa cggtgtaatc tttttaatag
     4741 gaagcccttc tttttgcgga tcgttttcag aaacaaaagg agacaggttt tctcctctca
     4801 ataaaattgc aagtcggtgt tcttttactg tttgaataat aatctgaaca ttattaatgc
     4861 ttttgatact ttcctgaatt ctagccacaa gatttctgtt ttcttctgtg ggaatccttc
     4921 cagcccttct atctgtgact gtatgattat catctatggt tgcaaaattg cccctgatgg
     4981 caaggtcgtt cttctccatt tccatcccta t
//
LOCUS       DAORYU010000097         4902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_11701_length_4902_cov_4.439653, whole genome shotgun sequence.
ACCESSION   DAORYU010000097 DAORYU010000000
VERSION     DAORYU010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4902
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11701_length_4902_cov_4.439653"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="PLW07_04195"
     CDS             complement(<1..701)
                     /locus_tag="PLW07_04195"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOL49757.1"
                     /translation="MKKITFFLKLFSLAGLIFLYILVACFVAIFSKRSSRRKNLCVNC
                     SYFTRVGLRYLGIKVTRYGHVSPFQNTSYLIVSNHVSYIDILIISSFFPAAFITSVEI
                     AKDIFLGTLARMGGSLFVERRKKSRLMKDLETISNVLEENLNVVLFPEGTSTNGEKIL
                     PFKKTLFRAAIKHGIPVLPLYIRYVSVDGIPVAINNRDLIYWYGNMKFFPHFTYLLKL
                     NSIRAELFILPEISSA"
     gene            complement(701..1609)
                     /locus_tag="PLW07_04200"
     CDS             complement(701..1609)
                     /locus_tag="PLW07_04200"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HOL49758.1"
                     /translation="MRKKMRQSIRRKITGGKTAIGKIKRVGKIVKNIHRTKIFHVSRL
                     KFGRFRPKIRIEIERGNFIIKTVENARELEQVLKLRFEVFYQELLDKKAVITKDIDEF
                     DFICDHLVIFDKEKQKYAGTYRLNLSIFSKRFYSAQEFNIKNFLNLPGIKLELGRACV
                     DKQYRTGVIISMLWRGLVEYIKLSGTRYLFGCSSVKTMDKKEIALLYLYLMEHFKAPD
                     ELMVEPTRNFRIPSFTEYKRQLILDDQSASVAQSLIPPLLNFYLKSGAFVCGEPALDK
                     SFKCVDFFTVLDMNNLKKSIEKKYDV"
     gene            1730..3169
                     /locus_tag="PLW07_04205"
     CDS             1730..3169
                     /locus_tag="PLW07_04205"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOL49759.1"
                     /translation="MNKIFIGIDVGTQGTRVVGIDENGNIQGIYEHAFILDPSDIHEG
                     WHQQNPEQWWNSTMLCLRKITSHIQIKKISAISVSSTSGTLCFLDKNYQPLIPAIMYN
                     DRRSEKEAMYLNENLKCLTDKMGYQFNSSYALAKILWVTKHLPDIFEKTVRIVHATDY
                     VIGKLSGNYCVSDYTNVLKTGYDLIEGKWPDDIENIFGVPLAKLPEVVPPGAVIGVTT
                     TECCNQTGVPSGIPVIAGMTDGCASQISTGAVSPGQWNSTLGTTLVIKGVTEQILKDP
                     AGRIYNHKHPDGYWLPGGASNTGGDCVTKRFKQEKWDYLNEKTADTIPTGKICWPLTG
                     KGERFPFVNPEAKGFQPDNCDEDILYAACLEGVGYIEKLAYQTLEGLGANISDTIRIA
                     GGATKSKIWNRIRASILNRTLVQPENPYAAFGAAILAASRTHFDNLQQACNAMVKIVC
                     EYQPEKTLVQKYQELYQKFLETLKEKQYI"
     gene            3179..4501
                     /locus_tag="PLW07_04210"
     CDS             3179..4501
                     /locus_tag="PLW07_04210"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOL49760.1"
                     /translation="MSIFKACDIRGTYGKELTDQISYKIGRAVGTLLKEKHIIVGGDL
                     RPSTQILKPAVIKGLIHSGCDVIDIGIVPTPVFYFAIKQLSADGGVQVTGSHNPPPDN
                     GMKIVLGKNPITPEEIKKIQSLVESENFATGNGKIELRNVVNDYKNYIKGLFEPENLT
                     IAVDAGNGCWWEIAPDVLQKMGYKVIKLFCQPDGTFPNRSPNPAVYKNLKAIQNLVKT
                     NNIDMAVAYDGDGDRAIFIDEKGSIVTTDIAIVLYIKLLLQQHKNIPVVYDIKCSQIV
                     RDEIINNGGTPIMEKSGHAFIKSTFLKTNAIFAGEISGHYFFKNIGGDDGLFATLMMA
                     KIIQEKGPLSQQIAKIPVYATSPDIRIPVNNPERILTKIENYYPAEMVNKLDGIRIQF
                     EHGWALIRKSVTEPIITMRFEGKTQTDLKNIKSEIFSLIPDIPTIDNE"
     gene            4494..>4902
                     /locus_tag="PLW07_04215"
     CDS             4494..>4902
                     /locus_tag="PLW07_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49761.1"
                     /translation="MNKYLFLCVITILFGFGYCEEHQPEVIVEKARTLIFNALQNNNQ
                     WQMYQSYVNYVSNILDKTKGTNWWNDKNGYFRLNTIDRWLRNPVDCIIEGEHISHSIH
                     QQCQSGIINTHQVIQTCTELLDINIESSPVSIKK"
BASE COUNT         1658 a          892 c          908 g         1444 t
ORIGIN      
        1 gctgatgaaa tttcaggtaa tataaaaagt tctgccctta ttgaattaag tttcaaaaga
       61 tatgtaaagt gagggaaaaa tttcatattc ccataccagt aaattaagtc gcgattgttt
      121 atagcaacag gtattccgtc tacagacaca tacctgatgt acagaggaag cactggaata
      181 ccatgtttta tcgcagcccg aaaaagagtt tttttgaatg gaagaatttt ttctccattt
      241 gtgcttgtac cttctggaaa cagaacaaca ttgagatttt cttccagtac attagaaatt
      301 gtttcaaggt ccttcatgag tcggcttttc ttccttcttt caacaaacaa acttcctccc
      361 atccttgcga gagtgccgag aaaaatatct tttgcaattt ccactgaagt aataaatgcc
      421 gcagggaaaa aagaagaaat aatcagaata tcaatgtaag aaacatgatt actgacaatg
      481 agataagatg tattttgaaa gggtgaaaca tgaccatacc ttgttacttt tataccaaga
      541 tacctgagac caactcttgt aaaataagaa cagttcacgc agagattttt tcgtctgctg
      601 gaacgctttg aaaaaatcgc aacgaagcat gccactaaaa tataaagaaa gataagacct
      661 gcaagagaaa aaagttttag aaaaaatgta attttcttca tcagacatcg tatttttttt
      721 cgatggactt tttcaaatta ttcatatcaa gcaccgtgaa aaaatccaca catttgaagg
      781 atttatcaag cgctggttct ccacatacaa aagcacctga tttaagataa aaatttagaa
      841 gtgggggaat caatgattgt gcaacagaag cagattggtc atcaagtatc aactgtcttt
      901 tgtattctgt aaatgaaggt atcctgaaat ttcttgtggg ttccaccatt aactcgtctg
      961 gtgctttaaa gtgttccatt aaataaagat ataaaagggc gatttccttt ttatccattg
     1021 ttttcacgct tgaacaacca aaaagatacc ttgtaccact gagttttatg tattcaacga
     1081 gcccgcgcca taacatactg ataatgacac ctgttctgta ctgtttgtca acacatgctc
     1141 ttccaagttc aagtttgata ccagggagat tgagaaaatt tttgatattg aattcctgcg
     1201 ccgaataaaa acgttttgag aagattgata gattgagcct gtatgtaccc gcatattttt
     1261 gtttttcttt atcaaaaatt accagatggt cacagataaa gtcaaattcg tctatatcct
     1321 ttgtaatcac tgctttttta tcaagaagtt cctgatagaa aacttcaaac ctgagtttca
     1381 acacctgttc aagttccctc gcgttttcaa ccgttttgat aatgaaatta ccccgctcta
     1441 tctctattct gattttgggt ctgaacctgc cgaatttcaa tcttgaaaca tggaaaattt
     1501 ttgttctgtg aatgtttttt acaatctttc ccaccctctt gatttttcca atagcggttt
     1561 tgccgcctgt tattttcctt cttattgact gcctcatttt tttgcgcact gcatttccct
     1621 ttttcatgtt tctggttttc ggttgtactg atgatatttt atcataaaga atgccagttt
     1681 ttatgtgata taatcaagaa ggtgtaaatt tgaacaatcc gccaaagcca tgaacaaaat
     1741 ttttattgga attgatgtcg gcactcaggg aacaagagtt gtgggcattg atgaaaatgg
     1801 gaatatccag ggtatttatg aacatgcttt tatactggac ccgtcggata ttcatgaagg
     1861 atggcaccag cagaatcctg aacaatggtg gaattcaaca atgttatgtt taagaaaaat
     1921 cacctcccat attcagatta aaaaaatatc tgccatttca gtatcatcaa catccggcac
     1981 attgtgtttt cttgataaaa actatcagcc attgattcct gccatcatgt ataatgacag
     2041 acgcagtgag aaagaagcaa tgtatcttaa tgaaaatctc aaatgtctta cagataaaat
     2101 gggttatcaa ttcaactcat catacgcttt agccaaaatt ttatgggtta caaaacattt
     2161 accggatatt tttgaaaaaa cagtaagaat tgtccatgca actgactatg tgattggaaa
     2221 actttcaggc aactattgtg tgagcgacta tacaaatgtt ctgaaaactg gctatgacct
     2281 tattgaagga aaatggcctg atgacattga aaatattttt ggtgtacctc tggcaaaatt
     2341 gccagaggtt gttccacctg gtgcagtaat tggcgtaacc actacagaat gctgtaatca
     2401 aacgggtgtt ccatctggca ttcctgttat tgcaggaatg actgatggat gcgcttctca
     2461 aatttccaca ggcgctgtct cgcccggaca atggaattcg actctgggaa caacccttgt
     2521 cataaaggga gtgacagagc aaatcctaaa agaccctgct ggcagaattt ataaccacaa
     2581 acatcctgac ggatactggt tgccaggcgg tgcaagcaat actggtggcg attgtgttac
     2641 aaaaagattt aagcaggaaa aatgggatta cctcaatgaa aaaactgcag atactattcc
     2701 aactggcaaa atttgctggc cgttgacagg aaaaggagaa agatttccct tcgtaaatcc
     2761 tgaagcaaaa ggcttccaac ctgacaattg tgacgaggac attttatatg ctgcctgtct
     2821 tgaaggagtt ggatatattg aaaaacttgc ctatcaaacc cttgaagggc ttggagcaaa
     2881 catttctgat accattagaa ttgcaggagg cgctacaaaa agcaaaatct ggaacagaat
     2941 aagagcgtca attcttaata gaacacttgt gcaaccagaa aatccttatg cagcatttgg
     3001 cgcagcaatt cttgcagcat cccgaaccca ttttgacaat cttcagcagg catgtaatgc
     3061 aatggtaaaa attgtctgcg aatatcaacc tgaaaaaaca ctggttcaaa agtaccaaga
     3121 attgtatcaa aagtttttag aaacattaaa agaaaaacaa tatatttgaa ggggacaaat
     3181 gtctatattt aaggcatgcg atatcagggg cacctatgga aaagaactga cagaccagat
     3241 ttcctataaa ataggaaggg ctgttggtac tttacttaaa gaaaagcaca ttatagtcgg
     3301 tggtgactta agacccagta cacagatttt aaaaccagca gttattaaag gacttataca
     3361 ttcgggttgc gatgttattg acattggcat tgttccaacc cctgtttttt atttcgcaat
     3421 taaacaattg tcagcagatg gtggcgtgca agtcacaggt tctcataatc caccaccaga
     3481 caatggcatg aaaattgttc tcgggaaaaa tccgattacg cctgaagaaa taaaaaaaat
     3541 tcagtcactt gtggagtcag aaaattttgc taccgggaat ggaaaaattg aattgagaaa
     3601 tgttgtaaat gattacaaaa attacattaa gggattattt gaaccagaaa atcttaccat
     3661 tgctgttgat gcgggaaatg gatgctggtg ggaaatcgca cctgatgtgt tacaaaaaat
     3721 gggatataag gtgataaaac ttttttgcca acccgatgga acatttccca acagatcccc
     3781 gaatccagcg gtttacaaaa acttaaaagc cattcaaaat cttgtaaaaa caaacaacat
     3841 tgacatggcg gttgcctatg atggagatgg agacagggca atctttattg atgagaaagg
     3901 cagcattgta acaacagaca ttgccattgt actgtatatc aaattgttgc tccagcaaca
     3961 taaaaatata ccagttgtat atgatattaa atgttctcaa attgtcaggg atgaaatcat
     4021 aaacaatggc ggcactccaa ttatggaaaa atcaggacac gcctttataa agtcaacgtt
     4081 tctaaagaca aatgccattt ttgcaggcga aatttcaggt cattactttt tcaaaaacat
     4141 tggtggagat gatgggcttt ttgcgacact gatgatggca aaaattattc aggaaaaagg
     4201 acctttatca caacaaatag caaaaatacc tgtgtacgca acaagccctg acattcgaat
     4261 cccggttaat aatcccgaaa gaatccttac gaaaattgaa aattattatc ctgcagaaat
     4321 ggtgaataaa cttgatggaa tacgcattca atttgaacac ggctgggctc tcataagaaa
     4381 atcagttaca gaacccatta tcaccatgcg atttgaagga aaaactcaaa ctgaccttaa
     4441 aaacataaaa agtgaaattt tttcgcttat ccctgatatc cctacaatag acaatgaata
     4501 aatatttatt tctgtgcgtt attacgatat tgtttggctt tggatactgt gaagaacatc
     4561 agccagaagt cattgtggaa aaagcaagga ctctgatatt taatgcattg caaaataata
     4621 atcaatggca gatgtatcaa tcctatgtca attatgtctc aaacattctt gataaaacta
     4681 agggaacaaa ctggtggaat gataaaaatg gttatttcag attgaatact attgatagat
     4741 ggcttcgaaa tccagttgat tgcattattg aaggagaaca catttcacat tcaatacacc
     4801 agcaatgcca atcaggcatt attaatactc atcaggtcat tcaaacctgc accgaacttc
     4861 tcgatataaa cattgaatcc agtcctgtat caataaaaaa ag
//
LOCUS       DAORYU010000098         4891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_11755_length_4891_cov_4.651158, whole genome shotgun sequence.
ACCESSION   DAORYU010000098 DAORYU010000000
VERSION     DAORYU010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4891
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11755_length_4891_cov_4.651158"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1498
                     /gene="cimA"
                     /locus_tag="PLW07_04220"
     CDS             <1..1498
                     /gene="cimA"
                     /locus_tag="PLW07_04220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HOL49762.1"
                     /translation="EKLDQFGFDFIEGGWPGSNPKAEEFFKKMASKKLKYSKIVAFGS
                     TRRKNLSADKDPFLKALVEANTDIIIIFGKSWDLHVRDVFRISLEDNLNLIGDSISYL
                     KSRGKTVFFDAEHFFDGYKENPEYAIKTLLVAESSGAERIVLCDTNGGALPFEVEEIV
                     RKVDRVLKTPIGIHAHNDSGTAVANTIAAVRAGAVHVHGTINGFGERCGNADLTAIIP
                     ILQIKMGFHCLDEEKLHHLTGLSRYIYETANIVPPGNQPFVGFSAFAHKGGVHVDAVG
                     KNPRTYEHIPPERVGNQRRVLVSELSGKSTILQKLGKYKLEKQPELIKKILDTVGELE
                     KKGYQFESAEASFELMVRKMLGLYKKPFHVEGFRVIVEERNAKVVAEATVKLKVGKSV
                     EYTVAEGNGPVNALDNALRKALIPFYPFLSSLKLTDYKVRILHPEKATGAVTRVLIES
                     MDEKESWGTIGLSENIIEASWMALVDGVEYKILKEIENFNQSSRQANS"
     gene            complement(1467..1976)
                     /locus_tag="PLW07_04225"
     CDS             complement(1467..1976)
                     /locus_tag="PLW07_04225"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOL49763.1"
                     /translation="MALCAPAEVEPITNEYYLPKVMDLIEKSETSIYVMMYNMTWYEK
                     YPDSPSNQLIDRLCKAARRKINVTVILNRDKPGSDRDADRNIQTAKILKKAEVKVLLD
                     PIEQITHAKIIIIDNRFVVVGSFNWSYYSLEKNNETAVVIDSKEIASYFLKYFNNVAV
                     RSSPVSTTD"
     gene            complement(2036..2893)
                     /locus_tag="PLW07_04230"
     CDS             complement(2036..2893)
                     /locus_tag="PLW07_04230"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HOL49764.1"
                     /translation="MIIIPAIDLKKSKVVRLYKGKFEKVTSYGLNPEEIVKDFILKGA
                     QRIHVVSLLGAKEGKTLDQDIRVIENMVKMRNLMAIRKCAIQIGGGIRRKDEVKQFFS
                     IGIDYVIIGTAIVLPQILEANFTISDISRAYAFADRKFFVNREIPEPDIMDKISNEIK
                     KRVIVSVDVSRDSVALSGWQVTIPIEPSWVISKLAEKNFSSFMITDTSRDGTLYGIDI
                     DVFARIINKVRKLTAKNLEFIVGGGIGSEQDIEIISQSNLPITGVVIGKALYEQKIDL
                     HAIIKQYQP"
     gene            complement(2871..4754)
                     /gene="topA"
                     /locus_tag="PLW07_04235"
     CDS             complement(2871..4754)
                     /gene="topA"
                     /locus_tag="PLW07_04235"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOL49765.1"
                     /translation="MSKKLVIVESPAKARTIGYMLGSDYIVRATLGHIMDLPENNFGV
                     DLESFTPEYIILPGKKKVVSYLKKLAANSEAIYIATDEDREGEAIAWHTASVLDKSIE
                     DVERVAFHEITKPAVLESFQKPRKIDMNLVSAQQTRRILDRIVGYTLSPLLGRNLSAG
                     RVQSVALQLIVTREKEIQNFIPQPYWVIKVVVDCRGKLFKMQLVSVDNEKIQSPGITD
                     KEKVAQYITDIKNSQQIIVSDVSRITKNTGPYPPFITSSLQQEASTKLGFASSKTMFV
                     AQQLYEGVPIEKKTPVGLITYMRTDSPSVSKIAQHQAAKFIQETFGNEYVPDRPPYYA
                     ARVSNAQEAHEAIRPTHINFTPDHLKKHLTRDQFLLYKMIWERFIASQMKPAVVETLT
                     IKANTGKYEFEAFKSKVVFDGFTKLWNIKIDAGEENIPDDISEATVLELKNTEAEEKK
                     TNPPPRYTEASLIRTLEKFGIGRPSTYAPTVSTLVKRKYVRKEKKVFVPEKLGVVVSE
                     TLSKYFPDVINVQFTAEMEKQLDEIAEGKKDGVVILKEFYGNFKPSLDKATAEMDTEI
                     KTIREEKNSPGPVEKIEKNCPKCGKPLIIRISRYGKFIGCTGFPKCRHTEKIKNNDHH
                     PSY"
BASE COUNT         1384 a          891 c          945 g         1671 t
ORIGIN      
        1 ggaaaaactt gaccagtttg gttttgattt tatcgaagga ggctggccgg gttcaaatcc
       61 caaggcagaa gaatttttta aaaaaatggc ttcgaaaaaa ttgaagtata gcaaaattgt
      121 tgcctttgga agcacaagga gaaagaacct ttctgcagat aaagatcctt ttttgaaagc
      181 acttgttgaa gccaacactg acattattat catttttgga aagtcctggg atttacatgt
      241 tagagacgta ttcaggattt ctcttgaaga caatcttaat cttataggtg actccatcag
      301 ttatttaaag tcaagaggaa agacagtttt ttttgacgct gagcatttct ttgatggtta
      361 taaagaaaat ccagagtatg caataaaaac tttgctagtg gccgaatcca gcggagcaga
      421 gagaattgtt ttatgtgata ccaatggtgg tgcgcttcct tttgaagtgg aagaaatagt
      481 cagaaaggtt gacagggttt tgaaaacacc aatagggatt catgcccaca atgattctgg
      541 tacagcagtt gcaaatacca ttgctgcagt tcgtgcagga gcagtacatg tgcatggaac
      601 catcaatggt tttggagaaa ggtgtggtaa cgcggatttg actgctatta ttccgatact
      661 gcagataaaa atgggtttcc actgccttga cgaagaaaaa ttacatcatc tgacaggtct
      721 ttcaaggtat atttatgaaa cagccaatat cgtgccgcca gggaatcagc catttgttgg
      781 gttcagtgct tttgcccata agggtggagt gcatgttgat gctgttggta aaaatcctcg
      841 tacctatgag catataccgc ctgaaagagt tggtaatcaa agaagggttc ttgtttcaga
      901 actttcgggt aagagtacaa ttctacaaaa actcgggaaa tataaactgg agaaacaacc
      961 ggaattgata aagaaaattc ttgacactgt gggagaactg gaaaaaaaag gttatcagtt
     1021 tgaatccgca gaagcttctt ttgaactcat ggtgagaaaa atgctggggt tatataaaaa
     1081 accttttcat gttgaaggat tcagggtaat agtggaagaa agaaatgcaa aggtagtggc
     1141 tgaagccaca gtgaaactta aagtaggtaa atcagtggaa tacacagttg cagaaggaaa
     1201 tggtcctgtt aatgctcttg ataacgcgct cagaaaagcg ctaattcctt tttatccatt
     1261 tctttcaagt ttaaaactga ccgattataa agtaagaatt cttcatccgg agaaagctac
     1321 cggagcagtc acaagggtac tgattgaatc catggatgaa aaagagagct ggggaaccat
     1381 agggctatct gaaaatatca ttgaagcatc ctggatggca ctggtggatg gggttgaata
     1441 caagattttg aaagaaatag aaaatttcaa tcagtcgtcg agacaggcga actcctgacc
     1501 gcaacattgt taaaatactt cagaaaatac gaagcaattt cttttgaatc aatcacaacg
     1561 gcagtttcat tgtttttttc aaggctgtaa taagaccagt taaagcttcc cacaacgacg
     1621 aatctattat ctatgatgat aatttttgca tgggtgattt gttctatcgg gtctaacaag
     1681 acttttactt ctgctttttt cagtatttta gctgtctgta tgtttctatc agcatctctg
     1741 tcggatccag gtttatccct gtttaaaatt actgtaacgt ttatttttct tctggcagct
     1801 ttgcataatc tgtcaatcaa ttggttggaa ggactgtcag ggtatttttc gtaccatgtc
     1861 atattgtaca tcatgacgta tattgaagtt tcagattttt ctattaaatc cattaccttt
     1921 ggcaaataat attcgttggt tattggttcc acttctgcag gcgcgcataa agccaatgat
     1981 tttgacacac aacatatgat tatcaatgtg aaaataaata tctttaaggt gtttttcatg
     2041 gttgatattg ttttatgatt gcatgcagat cgattttctg ttcataaagg gctttgccaa
     2101 tcacgacgcc agttattgga agatttgatt gtgaaattat ttctatgtcc tgttcagacc
     2161 cgatgccacc accaacaata aattcaaggt tttttgccgt aagtttcctg actttgttaa
     2221 taattctggc gaaaacatca atatcaattc cgtacagggt gccatctctg ctggtatcag
     2281 tgatcataaa ggatgaaaaa tttttttcag caagcttgct gataacccat gaaggttcta
     2341 ttggaatagt aacctgccag ccgctgagtg ctactgaatc tctggataca tcaacgctaa
     2401 caataactct ttttttaatt tcattactga ttttgtccat aatgtctggt tcaggaattt
     2461 ctctgttaac aaaaaacttt ctgtccgcaa acgcatatgc tctggaaatg tcactgatcg
     2521 tgaaatttgc ttcgagaatc tgaggtaaca cgatggcggt tccgatgatc acatagtcga
     2581 ttccaattga aaaaaactgt ttgacttcat cttttcttct gatacctcca ccgatttgta
     2641 tggcacattt tctgattgcc atcaaattcc tcatttttac catattttca attaccctga
     2701 tgtcctggtc aagtgttttc ccttcttttg cgccaagaag agagacaaca tgtattcgtt
     2761 gtgcgccctt taaaataaaa tccttgacaa tttcttcagg gtttaatcca taggacgtta
     2821 ccttttcaaa ttttccttta tatagccgaa caactttact ttttttgaga tcaatagctg
     2881 ggatgatgat cattgttttt aattttttct gtatgcctgc attttggaaa accagtacaa
     2941 ccgataaact ttccatacct gcttattcta attattaaag gtttgccaca tttcggacaa
     3001 tttttttcga ttttttcaac aggacccgga ctattctttt cttcccttat tgtcttaatt
     3061 tctgtatcca tttctgccgt tgctttatca agagaaggtt tgaaatttcc ataaaattct
     3121 tttaatataa caaccccatc ttttttccct tctgcgatct catcaagttg tttttccatt
     3181 tcagctgtaa attgtacatt tattacatca gggaagtatt ttgaaagtgt ttctgaaacg
     3241 acaacaccaa gtttttctgg aacaaacacc tttttttctt ttctgacata ttttcttttt
     3301 accaatgtgc tgactgtggg tgcatacgtt gagggtctgc caataccgaa tttttcaagt
     3361 gttctgatca gggatgcttc tgtatatctt gggggtggat ttgttttctt ttcttctgct
     3421 tcagtgttct tcagttcaag tactgttgct tcactgatgt catctgggat attttcttcg
     3481 cctgcatcaa tttttatgtt ccagagtttc gtgaatccgt caaacaccac ttttgatttg
     3541 aatgcttcaa attcgtattt ccctgtattt gcctttatcg tcagggtttc aacgacagca
     3601 ggtttcatct gacttgcgat aaatctttcc cagatcattt tatacagtaa aaactggtct
     3661 ctggtaagat gttttttcaa atggtccggt gtaaaattta tatgtgttgg tcttattgct
     3721 tcgtgcgctt cctgtgcatt tgatactctt gcggcataat aaggtggtct atcgggcaca
     3781 tattcattgc caaatgtttc ttgaataaat tttgcggcct ggtgctgggc gatttttgat
     3841 acagatggag aatctgttct catataagtg attaatccga caggtgtttt cttttcaatt
     3901 ggaacgcctt cgtataattg ttgcgctaca aacattgttt ttgaagatgc aaatcctaat
     3961 ttcgtagatg cttcctgctg aagcgaactt gtgataaagg ggggataagg acctgtattt
     4021 ttagttattc ttgaaacatc agaaacaatg atttgttgtg aatttttaat gtctgttatg
     4081 tattgcgcta ccttttcctt atccgtaata ccaggggact gaattttttc gttgtcaaca
     4141 gacaccaact gcattttaaa taatttaccg cgacaatcca caacgacttt tatgacccag
     4201 tagggttgag gaataaaatt ttgaatttct ttttcccttg ttacaatcag ttgtagtgca
     4261 actgactgga ctcttcccgc cgaaagattt cttccaagca aagggctcag ggtgtatccc
     4321 acaattctgt caagaattct tctcgtttgt tgcgccgaaa caagattcat gtcaattttt
     4381 cttggttttt gaaaagattc aagaaccgct ggttttgtaa tttcatggaa tgcgaccctt
     4441 tctacatcct caatactctt atcaagaaca cttgcagtat gccatgcaat tgcctcaccc
     4501 tctctatcct catcggtcgc tatatatatt gcttcagagt ttgccgcaag tttttttaaa
     4561 tacgatacaa cctttttttt accaggaagt attatgtatt caggagtaaa actttcaagg
     4621 tcaaccccga aattattttc tggaaggtcc ataatatgcc cgagcgttgc tcttacaata
     4681 tagtcagaac cgagcatgta tcctattgtc cgcgcttttg ccggagattc cacaattaca
     4741 agttttttgg acatgtgttt atcctttcaa ttgcattatt ataatacttt tggtaaaatt
     4801 attaaatacg tgtattttga caacaggtag aggatatatg aaatacatat ggagtggacg
     4861 attaaaaaaa gatcttcacc atcttgtcag g
//
LOCUS       DAORYU010000099         4826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_11949_length_4826_cov_4.700063, whole genome shotgun sequence.
ACCESSION   DAORYU010000099 DAORYU010000000
VERSION     DAORYU010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11949_length_4826_cov_4.700063"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3423
                     /locus_tag="PLW07_04240"
     CDS             <1..3423
                     /locus_tag="PLW07_04240"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF020121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOL49766.1"
                     /translation="EKWAKPYAGGTTRMLFVYRNHVLVHLREIMQRGDFVVSLLATTY
                     EKPYEQMREIPSKIKDFKPDVMFFAAFDWKSGFPPGVIDIIKNWVYRGGGLFVDADLS
                     NDAYLPLLELVKEGKEIKPDGFLSGSPFSLPEMKFYELGSGRIVILKGGVFTKGDMLS
                     IGEWRPNPWMPGWEYNYPHLISAILWAAHKEMQVRFSPIKSEKDVFFVTCAGEKMKGK
                     VNAIVEIRNQYYSLYSSSQYTFSLSDSEAVIPVAKMAELQEGISVFKIVLSDAKGRVI
                     GWTSGKLERSATIKADIVCDHPNNTYFRNEPVKITVNMSVLGSEKKDIEGILTISDAW
                     NRTIWKSDIRGAEILTLCPDMSLVLDTWHQIILRVCRNEKTVAEQRQLLFIFPDKNIP
                     DDDFSVGCWGHPAGNPSGAVVTTRSAVENGIDYFYSYGGEMARDHVYRNHGHIYGPPR
                     INDNLFTSLRIKSRDPVTLVMTPSLYPSVFEWEKVKKDVMTKTDDFVRNLGASVMMLD
                     DERDLKGDYDWSETTLAHFPEWLKKEYGTISNLNRVWNRSYRSFEEVRPERKENLSQD
                     NLAPYIDFRKYIGWIVEEFYTRQPLLWVKEVNQKATVGMHGIYTTSSSRPWDMSKVIP
                     LLSITGRYDGILEEWFRGMGKNCIHGQYTGYEMLGKLSYDNRITPWKNLFHGSRWVLY
                     YQMRNLVAPGGIFQSIINYDGTVREIYRNLYGDELSEIKQGIGKLVLNAKPVTDGIIF
                     TYSYTSCLFNRHTTSYFSAKTLVQDIGYQHDLISYAQLENGQIPASTRILFLADCISM
                     SDAEIESVKKFVSSGGIVIADAQTAIYDQHGVKYSPSPIDGIFGIDRSNIRYEPEKKT
                     VIFGSQQLSVWVAESGIKLLPSAISKGRTQDGQPVVIINSYGKGQAVYLNMHLVPYAS
                     LAASGAAGEIVVEQPGAKEIEISYQKIFRFIVEDLCGIKPVVNVSPTTSCKEVFLFTG
                     KQGKTALVGILPFPRLQNKTKISISLPSFPGITTMYDVREKKQYKSGKKFEYIINPDR
                     TGLLAFMPYSVTKIVIDGPKRCWQGGIFKFDGKIVTSSGIADGHVVRIQVFDSRNREI
                     PFWWRTICADGNIFNVSLPVDLSMTYGQYKMVATDIISGVKGTTVFMISNKK"
     gene            3420..>4826
                     /locus_tag="PLW07_04245"
     CDS             3420..>4826
                     /locus_tag="PLW07_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49767.1"
                     /translation="MTKLFPTDKQLFARQDIFLKPGLAVIPENAELLPCARYLIERLE
                     NKGFPDARLSSEGAGDRIILSLAPSISETFQIQLKKDSDAITFEISGNSLASCYYGVY
                     AFLQHAKVNDKVLVTRLKSYMGSTSNPHRNLIVSLSWMENLIEFPFYGFEQWKKFIEL
                     VSELRFHRIDFMQWGCTIPDPPAAGTKGEDEWEMWQDGSIQKNDWPIPESYRGLQYQE
                     GGWRNRCVFEPWLFPIAGKTIKNSALFSVCYPPEKTIPLCIWDTGKQKLVHRLWRPPF
                     IKDEKLFRKITDFIHNHGMKTGLFTTPRVPCARDEKNFEAYWKQVIDFFTSQGIDDFL
                     FETEEGPLSFEHHSRCEFCQKTFGDIFAGYTRKIARQTYILGELIRGKSKHSNVGWIL
                     HVPLHAGYGNPVERRKWLESPDNYIENLNIFKANAPEDFTLDYVPFPGEKNITHEFLP
                     QIYFDVFGKNRIRPTGYTH"
BASE COUNT         1494 a          842 c         1089 g         1401 t
ORIGIN      
        1 gagaaatggg caaaaccata tgctggtggc actacaagga tgctctttgt ttaccgtaac
       61 catgttcttg ttcaccttcg tgagattatg caacgcggtg actttgttgt gtccctgctt
      121 gccacaactt atgaaaaacc ctatgagcag atgagggaaa ttccttcaaa gataaaggat
      181 tttaagcctg atgtaatgtt ttttgcggcg tttgactgga aatcagggtt tccaccagga
      241 gtgattgaca tcatcaaaaa ctgggtatat cgtgggggcg gactttttgt ggacgctgat
      301 ttatcaaatg atgcctacct gcctttgtta gaacttgtaa aagaaggaaa agagataaaa
      361 ccagatggat ttttgtctgg ttcccctttt tcattgccag agatgaaatt ttatgaactc
      421 ggtagtggta gaattgtaat tcttaaagga ggtgttttca caaaaggtga tatgctgtcc
      481 atcggagaat ggagaccgaa tccctggatg cctggctggg aatacaatta tccacatctg
      541 atttctgcga ttttatgggc tgctcataaa gaaatgcagg taagattcag tccaataaaa
      601 tcagaaaagg atgtgttttt cgttacatgt gccggtgaaa aaatgaaggg taaggttaat
      661 gccattgtgg agatacgaaa ccagtattat tctctttatt cctcatcaca gtacacattt
      721 tcgctttcgg attctgaagc agtcattcct gttgcaaaaa tggcagaact tcaggaaggt
      781 atttcggttt ttaagattgt attatcagat gcaaagggca gagttattgg ctggacatca
      841 ggaaaactgg aaagatcagc cacaataaaa gccgacattg tgtgcgacca tccgaataac
      901 acatatttca ggaatgaacc agtaaaaatt actgtgaata tgagtgtgct ggggtctgaa
      961 aaaaaggata tagaaggaat attaactatt agtgatgcct ggaatagaac tatctggaaa
     1021 agtgatatcc gtggggcaga aatacttacg ttgtgtcctg acatgtctct tgtacttgat
     1081 acatggcacc agataatatt gcgagtgtgc agaaatgaaa aaactgtagc tgaacagagg
     1141 caacttcttt ttattttccc ggataagaac attcctgatg atgacttttc agttggttgc
     1201 tggggacacc ctgctggcaa tccatcaggt gctgtggtta caacccgttc agcagtggaa
     1261 aatggtattg attattttta cagttatggt ggagaaatgg caagagatca tgtgtatcgg
     1321 aaccacggac atatttatgg gcctccaaga attaacgaca atctttttac aagtttacgg
     1381 attaaatcac gagaccctgt aacacttgtt atgacgcctt ccctgtatcc ctctgtattt
     1441 gaatgggaaa aggtaaaaaa agatgtgatg acaaaaactg atgattttgt caggaattta
     1501 ggtgccagtg tgatgatgct tgatgatgag agagacctta aaggtgacta cgactggtca
     1561 gaaaccacgc ttgctcattt tcctgaatgg ttgaaaaaag aatacggaac aatatcaaat
     1621 ctgaacaggg tatggaacag gagttatcgc tcatttgaag aggttcggcc tgaaagaaaa
     1681 gaaaacttaa gccaggacaa tcttgctcct tacatcgact ttcggaaata catcggatgg
     1741 attgttgagg aattttatac aaggcaaccc ctgctatggg tgaaagaggt aaatcaaaag
     1801 gcaacagttg gaatgcacgg catctatacg acatcatctt cgcgaccatg ggatatgtca
     1861 aaggttattc cattgttatc aatcactgga agatatgatg gaattcttga agagtggttc
     1921 agaggcatgg ggaaaaattg catacatggg cagtatacag ggtacgaaat gcttgggaaa
     1981 ttgtcatatg ataacaggat tacaccatgg aaaaacctgt ttcatggttc caggtgggtt
     2041 ctgtattatc agatgcgaaa tctggttgct ccaggtggaa tttttcagtc aataataaac
     2101 tatgatggaa ctgtaaggga aatttacaga aacctctacg gggatgaact cagtgaaatt
     2161 aaacagggta tcggaaaatt ggttttaaac gcaaagcctg ttacggatgg tattattttt
     2221 acgtattcat atacgagctg tctttttaac aggcatacga cttcttattt ttctgccaaa
     2281 acacttgttc aggacatcgg ttatcagcac gacctgattt cttatgctca acttgaaaat
     2341 ggtcaaattc ctgcttctac cagaatactg tttcttgcag attgcattag tatgtctgat
     2401 gctgaaattg aatctgtgaa gaagtttgtt tcttctggag gaatagtaat agcagatgct
     2461 caaacagcga tttacgacca gcatggagta aagtacagtc cttcaccgat agatgggatt
     2521 tttggaatcg accgttcaaa catcaggtat gaacctgaga aaaaaactgt gatatttggt
     2581 tcgcagcagt tatctgtgtg ggttgctgaa tcaggtataa aacttcttcc ctctgcaatt
     2641 tccaaaggca gaactcagga cggacaacct gttgtcataa taaattctta tggtaaagga
     2701 caggcagtgt atcttaatat gcaccttgtt ccttatgcga gtcttgctgc ctcaggtgca
     2761 gcgggagaga ttgttgttga acagccagga gcaaaggaaa tagaaatcag ttatcagaaa
     2821 attttcaggt tcattgttga agatttgtgc ggaatcaaac cagtggtcaa tgtatcacca
     2881 acaacatctt gcaaagaagt tttcctgttc acagggaaac agggtaagac ggctcttgtt
     2941 ggaattttac catttccaag attacagaac aaaacgaaaa tatctatttc tttaccatct
     3001 tttcctggca taacgaccat gtatgatgtg cgcgagaaaa aacaatataa gagtggaaaa
     3061 aagtttgagt atattataaa tcctgacaga actggcttgc tggcttttat gccatattca
     3121 gtaacgaaaa ttgttataga tggtcccaaa cgctgctggc agggtggcat atttaagttt
     3181 gatggaaaaa tcgtaacatc atcaggcatt gctgacggac atgtggtcag gattcaggtt
     3241 tttgacagcc gtaacaggga aattccattc tggtggcgca caatatgtgc tgacggaaat
     3301 atcttcaatg tttcattgcc tgtagacctt tcaatgacat acggtcagta taaaatggtt
     3361 gccacagata ttatatcagg ggtgaaaggt acaactgtat tcatgatttc aaataaaaaa
     3421 tgacaaaact tttcccaact gataaacagt tgtttgcaag acaggacatc tttttgaaac
     3481 ctggtcttgc tgtgatacca gaaaatgctg aattgttgcc atgcgcaaga tatctgattg
     3541 aacgcttgga aaacaagggt tttccggatg caagattatc cagcgaaggt gccggtgaca
     3601 gaataatttt atccctggca ccatccatca gtgagacatt tcaaattcaa ttaaaaaaag
     3661 attctgacgc tattacgttt gaaatttcag gaaatagtct ggcttcatgt tattatggag
     3721 tatatgcctt cttacagcat gcgaaggtca atgataaggt gctggtcaca cggcttaagt
     3781 cttacatggg tagcacaagt aacccgcatc gcaatctgat tgtttccctg tcgtggatgg
     3841 aaaacctgat cgagtttccg ttttatggtt ttgaacagtg gaaaaaattt attgaacttg
     3901 tttcagaact cagatttcat cgcatagatt ttatgcaatg gggctgcacc attcctgacc
     3961 cgccagcagc gggaacaaaa ggtgaagatg aatgggaaat gtggcaggat ggttcaatac
     4021 aaaagaatga ctggccaatt cctgaatctt accgtgggct gcaatatcag gaaggcggat
     4081 ggagaaaccg ttgtgttttt gaaccgtggt tatttccaat tgctgggaaa acaatcaaaa
     4141 attctgcatt gttcagtgtt tgttatccac ctgaaaaaac aatacctttg tgcatatggg
     4201 atactggtaa acaaaaactt gtccacagat tatggcggcc accgtttatc aaagatgaaa
     4261 aattattcag gaaaatcaca gattttattc ataatcatgg aatgaaaaca ggccttttca
     4321 caaccccgcg tgttccgtgt gcgagagatg agaaaaattt tgaagcatac tggaaacagg
     4381 tgattgactt tttcaccagc cagggaattg atgattttct ttttgaaaca gaagaaggcc
     4441 cgctttcttt tgaacatcat agtaggtgtg aattttgtca gaaaacgttc ggcgacattt
     4501 ttgcaggata cacaaggaaa attgccaggc agacatatat tcttggtgaa cttatcaggg
     4561 gaaaaagcaa acattcaaat gtgggatgga ttcttcatgt tccattacat gctggctatg
     4621 gaaatcctgt ggaacggaga aaatggcttg aaagccctga caattacatt gaaaatctga
     4681 atatctttaa ggccaatgct ccagaagatt ttacgcttga ttatgttccg tttcctggag
     4741 aaaaaaatat cacgcacgag tttcttcccc aaatttattt tgatgtgttt gggaaaaatc
     4801 gcatcagacc tacaggatat actcat
//
LOCUS       DAORYU010000100         4826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_11950_length_4826_cov_4.338713, whole genome shotgun sequence.
ACCESSION   DAORYU010000100 DAORYU010000000
VERSION     DAORYU010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11950_length_4826_cov_4.338713"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1406)
                     /locus_tag="PLW07_04250"
     CDS             complement(<1..1406)
                     /locus_tag="PLW07_04250"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOL49768.1"
                     /translation="MYKKLTEIIVSEIEGIAGKENVLREKEKLIDYSHDEFALPEVFN
                     FPEIVVKVINTQQVAEIVKLATCEKIPVVARGGGTGLCGGCIPAYGGIVISTEKMNKI
                     CKIDQNNLMAHLEAGVTLKEFYQALKTKKLFFPPHPGEESATIGGLVSTNAGGARAVK
                     YGTIRNFVRGLTAVCSDGKIIQAGGKIIKDSTGYNLVQLFTGSEGTLGIITEAILSIM
                     PEPEAYLTLIVPFNNTKDTLNCVPSILKSSVRPLAIEFISKQIISIAEDYCGKHWPSM
                     PGEVFLMVILEGKNSEVERDCETMAQICMENKAVDVFVAQAEEKQQDILHVRSIVYQA
                     LKSSTIEILDVTVPTTEMAYHIDFVEQLSKTYNLWLPTYGHTGDGNLHTHITKKTPDG
                     KEIKNWKEIYPKIRQSIIADGIKRGGKISGEHGIGLVKKQYLDMCFDADYIEMLKKIK
                     RAFDPFFIFNPGKIFDPE"
     gene            complement(1399..2070)
                     /locus_tag="PLW07_04255"
     CDS             complement(1399..2070)
                     /locus_tag="PLW07_04255"
                     /inference="COORDINATES: protein motif:HMM:NF021759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HOL49769.1"
                     /translation="MIHLKKFLEEKTDKKIHIEVSEDRKNLVQIITKQDEKIVKIHQN
                     LVNAPHPIIRAIADFITGKEKRKNKKTIVEYARKNPHLFSKQRKMNIDVTGKFYNLKQ
                     VFDKINEQFFNGSLSSVNISFGKRYYSKRHRSIVFGNYCPETNLIRINRSLDSDSVPE
                     FFLEYIVFHEMLHAYLYLLDSGSTGHTKKFKMMEKELPNLEKAEKWKRENLSLFIGSK
                     KKKHV"
     gene            complement(2077..2628)
                     /locus_tag="PLW07_04260"
     CDS             complement(2077..2628)
                     /locus_tag="PLW07_04260"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOL49770.1"
                     /translation="MAKKYIETSLTNLVRARPIIIIVTLHPDGGVNAGTFGAYTNVSG
                     SEIGIAIGKPSHTYKNIKRTGELTINVVTRPIAIAAEICGQNIPPTKSEIEESGLTTE
                     PSKKISTPIIKECVANLECKYEKEIEINYHSFVIVKCIAGHIDEEYIADDGGLDVVKA
                     QAVFNIGYPEPVYATLGNVFFVK"
     gene            complement(2647..3729)
                     /locus_tag="PLW07_04265"
     CDS             complement(2647..3729)
                     /locus_tag="PLW07_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOL49771.1"
                     /translation="MTDRECFYASMNYKERNRHPYWDLFGPWQETLERWKKEGYDGID
                     DFGADKLIQVRHIFDPYPAFEKKVLSEDADHRIIINEDGVVMKEFKKYEKSSMPQFIK
                     FPVETPSEFKEFWKQRMKSDVSLRLGPEWKQQLLTAEKSDCPFFVTADRWVGFFGPLR
                     SLVGVEKLCTLFYDEPAFVEEMMECFLKHTITTMKQVLEIVHVDILIIWEDMAYHTGP
                     LISPSMVKSFMFPRYRKLVDFVKNYGKKVSFIALDSDGNVDSLIPIWIESGIDIIYPF
                     EVQAGMDVVALRKKYGKSLRMWGGFDKRIVAKGKRTIDQETKRLAGLVEEGGYICGMD
                     HSAPPDISFENFRYFMTKMKEISGKR"
     gene            complement(3744..4436)
                     /locus_tag="PLW07_04270"
     CDS             complement(3744..4436)
                     /locus_tag="PLW07_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013748248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOL49772.1"
                     /translation="MSFKKILPAFFIFTIACVFTSAQEKKQDVLEKTTVIVTLPAPEI
                     KGKLSVEEALAKRRSVRQYKNNPLSITELGQILWAGQGITSNWGGRTAPSAGATYPLE
                     IYVVVGNVALLEPAIYHYDVLAHGLKKVSSSRDVREKLASAALGQQSIKQAPATIVIA
                     ADFSRTERRYGQRAQQYVFIEVGHVAENIYLQAESLGLGTVAIGAFNDTMVREILEIP
                     QDVVYLMPVGKK"
     gene            complement(4452..>4826)
                     /locus_tag="PLW07_04275"
     CDS             complement(4452..>4826)
                     /locus_tag="PLW07_04275"
                     /inference="COORDINATES: protein motif:HMM:NF016382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HOL49773.1"
                     /translation="NADVFIVDGHGIAHPERMGLASYVGVCIKKPVIGCAKSLLIGKY
                     KMPGNEKGNYTYITDEGEIIGAAVRTKPDTKVVFVSPGNKIDLETSMKIILACTGNYR
                     LPQPLRIAHALARKKAKTLQIS"
BASE COUNT         1319 a         1006 c          818 g         1683 t
ORIGIN      
        1 cattcagggt caaagatttt tcctggatta aaaataaaga aagggtcaaa tgcccttttg
       61 atttttttca acatttcaat gtaatcagca tcaaagcaca tgtcaagata ctgctttttt
      121 accagaccta tgccatgttc gcctgatatc tttcctccac gttttattcc atcagcgata
      181 atggattgtc tgatttttgg ataaatttct ttccagtttt taatctcttt tccatcaggt
      241 gttttcttcg taatatgtgt atggagattg ccgtctcccg tatgtccata agttggaagc
      301 cagaggttat aagtttttga aagttgttct acaaaatcta tgtgatacgc catttcagtc
      361 gtcggtactg tgacatccag aatttcaatg gtactggatt ttaatgcctg atatacaatg
      421 cttctaacat gaagaatgtc ctgttgtttt tcttctgcct gcgcgacaaa tacatcaact
      481 gctttatttt ccatacaaat ctgcgccatt gtttcacagt cgcgttctac ctcactgttt
      541 tttccttcaa gaataaccat aagaaaaact tctccaggca ttgatggcca gtgtttgcca
      601 cagtaatcct cggcaatgga aataatttgc ttgctgataa attcaatagc aagtggcctt
      661 acagaagatt tcaggataga aggaacgcaa ttgagggtat cttttgtatt gttaaatgga
      721 acaatgagtg taagatatgc ttctggttct ggcatgatgc tgaggattgc ttctgtaata
      781 atacccagtg ttccttcaga accagtgaaa agttgaacaa ggttgtaacc agtactgtct
      841 ttaataattt tgcctcctgc ctgaatgatt tttccatcag aacagaccgc ggttaatccc
      901 ctgacaaagt ttcttattgt tccatatttt actgctcttg caccaccagc atttgtactg
      961 accaggccac caatggttgc gctttcttct ccaggatgtg gcgggaaaaa caattttttt
     1021 gtttttagtg cctgatagaa ttctttcaat gttacacctg cttcaagatg agccataaga
     1081 ttattctggt cgattttaca aattttattc attttttctg tgcttattac aatacctcca
     1141 taagcaggaa tgcatccacc acataatccg gttccaccac cccttgcaac cacagggatt
     1201 ttttcgcatg ttgcaagttt tacaatttct gcgacctgtt gtgtgtttat aactttcaca
     1261 acaatttctg ggaaattgaa tacttctggg agggcaaatt catcgtgact ataatctata
     1321 agtttttctt tttctctcaa gacattttcc ttgcccgcga ttccttcaat ttctgaaact
     1381 atgatctctg tcaatttttt atacatgttt tttctttttt gaaccaatga acaaagaaag
     1441 attttccctt ttccattttt ctgctttttc aaggtttgga agttctttct ccatcatttt
     1501 gaattttttt gtgtgacctg tggagcctga atctaaaagg tataaatatg cgtgtaacat
     1561 ttcgtggaaa acaatgtact caagaaaaaa ttcaggcact gaatcactat caaggcttct
     1621 gttaattctg ataagattgg tttcagggca gtaattacca aacacgattg aacggtgcct
     1681 tttgctgtaa tatcgttttc cgaaacttat attcacagaa gataaggaac cattgaaaaa
     1741 ctgttcgttg attttgtcga aaacctgttt cagattgtaa aattttcctg taacatcaat
     1801 gttcattttg cgctgttttg aaaaaagatg ggggtttttt cttgcatatt caacgatcgt
     1861 ttttttgttt tttctttttt cttttcctgt aatgaaatcc gcgattgctc tgataatcgg
     1921 atgcggtgcg ttaacaagat tttgatgaat ttttacgatt ttttcatcct gttttgtgat
     1981 gatctgaaca agattttttc tatcttctga aacctcaata tgaatttttt tgtctgtttt
     2041 ttcctcaaga aactttttca gatgtatcat ggattcctac ttgacgaaaa agacgttgcc
     2101 gagcgtcgca tagacaggtt caggataacc gatattgaat actgcctgcg cttttacaac
     2161 atcaagacca ccatcatcag cgatatattc ttcatcaata tggcccgcaa tacacttaac
     2221 aatgacaaac gaatgataat tgatttcaat ttctttttca tatttacatt ctaagtttgc
     2281 cacgcactct ttgataatgg gagtagaaat tttttttgac ggctctgtgg taaggcctga
     2341 ctcttcaatt tcactttttg tcggtggaat attctgacca caaatttctg ctgcgatggc
     2401 aataggtctt gtcacaacat tgattgtgag ttcacctgtc cttttgatat ttttgtaagt
     2461 atgagatggc ttgccaatcg caataccgat ttctgaacca gaaacattcg tataagcgcc
     2521 aaatgttcca gcattgacac ctccatctgg atgaagggtt acaataatga tgattggtct
     2581 tgccctcaca agatttgtta gagatgtttc aatatatttt tttgccatat cctacctcct
     2641 gaatgattac ctttttccac tgatttcttt catcttcgtc ataaaatatc tgaagttttc
     2701 aaatgatatg tcaggtggtg cgctatggtc catgccgcag atataaccgc cttcttcaac
     2761 aagccctgct aatctttttg tttcctggtc tattgtccgt ttgccttttg cgacaattct
     2821 tttatcaaat cctccccaca ttctcaatga ttttccatac ttttttctta aggcaaccac
     2881 atccattcct gcctgtacct caaaaggata aataatgtct atgcccgatt caatccagat
     2941 gggaataagc gaatccacat taccgtcgct atcaagcgca ataaaagaaa cctttttacc
     3001 atagtttttt acaaaatcca caagtttcct gtatctggga aacatgaagg acttcaccat
     3061 tgagggtgaa ataagaggtc ctgtgtgata tgccatgtcc tcccatataa tcagaatatc
     3121 aacatggaca atttcaagaa cctgtttcat tgtggtaata gtgtgtttca gaaaacattc
     3181 catcatttct tcaacaaacg caggttcatc ataaaatagt gtacagagtt tttcaactcc
     3241 cacaagactc cttaatggtc caaaaaatcc cacccatcta tcggcagtaa caaaaaacgg
     3301 gcaatcactt ttttcagccg tgagtaattg ttgtttccat tcaggaccca gtcttaaaga
     3361 aacatcagat ttcatccttt gtttccagaa ctctttgaat tcgctgggcg tttcaacagg
     3421 aaattttata aactgtggca ttgaactttt ttcgtatttt ttgaattcct tcatcacgac
     3481 cccatcttca ttgataataa ttctgtggtc agcatcttct gacagaacct ttttttcaaa
     3541 ggcaggatac gggtcaaaaa tatggcgaac ctgaatgagt ttatctgcac caaaatcatc
     3601 tataccgtca tacccttctt ttttccatct ttccagtgtc tcctgccacg gtccaaaaag
     3661 gtcccagtat ggatgacgat ttctttcttt gtaattcatt gaagcataga aacattccct
     3721 gtctgtcata cattcctcca aaattatttt ttcccgacag gcattaaata cacaacatcc
     3781 tgagggattt ccaaaatttc tcttaccatt gtatcattaa aagcaccaat cgcaacagtt
     3841 cccagtccaa gagattctgc ctgcagataa atgttttctg ctacatgtcc aacttcaata
     3901 aatacatatt gttgtgctcg ttggccatat cgtcgttcag ttctggaaaa gtcagcggca
     3961 atgacaattg ttgcaggtgc ctgttttatt gattgttgtc cgagtgccgc agatgcaagt
     4021 ttttctcgaa catcccggga tgaagaaacc tttttcaaac catgtgcaag cacatcgtaa
     4081 tggtatattg ctggttcaag taacgcaaca tttcccacaa caacataaat ttctaatgga
     4141 tatgttgcac ctgcagatgg agctgttctt ccgccccagt tcgaagttat gccctgaccc
     4201 gcccacaaaa tctgaccaag ttctgtaatg cttaacgggt tatttttata ctgtctgaca
     4261 ctgcgtcttt tcgcaagcgc ttcttcaacc gataatttcc cttttatttc aggagcaggt
     4321 aatgttacaa tgacagttgt tttttctaaa acatcctgct ttttttcctg cgcagaggtg
     4381 aaaacacagg caatggtaaa aatgaaaaat gcaggaagaa tttttttgaa cgacatctgc
     4441 tacctccttt tttacgatat ttgaagtgtt tttgctttct ttcttgcaag tgcatgcgca
     4501 attctcaacg gttgtggtaa tctataatta ccagtgcatg caagaataat tttcatagaa
     4561 gtttcaaggt caattttatt tccaggagaa acaaaaacga cttttgtatc aggtttagtt
     4621 cttactgctg ctccaataat ttctccttca tctgtgatat aggtataatt acccttttca
     4681 tttcctggca ttttatattt tccaattagc agggattttg cacatcctat cacgggtttt
     4741 ttgatacaaa ctccgacata actggcaagc cccattcttt ctggatgcgc aatgccatga
     4801 ccatcaacaa tgaagacatc agcatt
//
LOCUS       DAORYU010000101         4810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_12001_length_4810_cov_4.105783, whole genome shotgun sequence.
ACCESSION   DAORYU010000101 DAORYU010000000
VERSION     DAORYU010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12001_length_4810_cov_4.105783"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="PLW07_04280"
     CDS             complement(<1..341)
                     /locus_tag="PLW07_04280"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOL49774.1"
                     /translation="MRRIAIINQKGGSGKTTTVVNLGAAVTEKRFRVLLIDLDPQAHT
                     TIHLGFEPFKLEKSIYDVLVNEVNPDEVILETHVKNLFLLPAKIELASAEIEMVNTIG
                     REIVLRESLKKS"
     gene            658..1536
                     /gene="aroE"
                     /locus_tag="PLW07_04285"
     CDS             658..1536
                     /gene="aroE"
                     /locus_tag="PLW07_04285"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOL49775.1"
                     /translation="MIITGATIITGVFGYPVRHSLSPVFQNAAFQYRHLNWVYVPFEI
                     SPEYLKDAVRAIKIFSVKGINVTIPHKKEVIKYLDVLDEEVKLLGVANTIVNEDGCLK
                     GYSTDGEGFLRSLKEDGGFTPENKLVFLFGSGGSSFAIAGALVRAGISGLIICNRTIE
                     KAAVLKNHLKDCFSFQNIEVIPFEKRDNAGIWKNIDLVVNTTSVGMKDDDIVLVDEKN
                     LNDNMFIYDIVYNRETPLILSAKKKGVRCLDGLSMLVFQGAVSFTLWTKIEAPIEVMK
                     KSLIAYKKTMQKGNQN"
     gene            1542..2036
                     /locus_tag="PLW07_04290"
     CDS             1542..2036
                     /locus_tag="PLW07_04290"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOL49776.1"
                     /translation="MLAGFNGTWRKEAGIILASRLGRKFFDIEEMIEKRENDRIAHIS
                     QIKGAAYVRDLENSIIEEISVFDNCVISIGPDVIANQKNRLFFKSNGIVIWFTAEPSV
                     ILLRLHPGKESKNLLKRQNAISHIRQILKEHDYSCFADKIIDTSSLTSEESADRVQEM
                     LALF"
     gene            complement(2022..3176)
                     /gene="aroC"
                     /locus_tag="PLW07_04295"
     CDS             complement(2022..3176)
                     /gene="aroC"
                     /locus_tag="PLW07_04295"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOL49777.1"
                     /translation="MLGNTFGRIFRITTCGESYGKGKGSALAMIVDGVPPGLKITREY
                     IQKELDKRKPGIGKLNSPRKETDQCEIFAGIGQDGVTTGAPLGIIIYNVDTKKIHVEQ
                     YRSYKDIFRPGHATYPFFLKYGEYQDWCGAGRASGRETVARVAGGAVAKFILEREGIE
                     ILAYTKECMGIRAREMPFEEIKKNYRKNEINCPDLEAAKKMEEKVLEIKNQGDTAGGI
                     IEVIAHNVPPGLGEPVFDKINALIAHAICSIGAVKGIEFGAGFRVGNMKGSENNDQPY
                     IDSHGKIRFRTNNAGGILGGITNGEDIVVRIAVKPTPTISIEQTSINMKKLKEEKLKP
                     ITRRDPTLLGRIYAVAEAMMSIAILDALYMAKAYDALARLDKKWLTLKER"
     gene            complement(3188..4468)
                     /gene="aroA"
                     /locus_tag="PLW07_04300"
     CDS             complement(3188..4468)
                     /gene="aroA"
                     /locus_tag="PLW07_04300"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOL49778.1"
                     /translation="MNLKINKTQCLNGIAVIPASKSHTIRAVVIASLAAGNSRIINPL
                     DSLDTMAAVNACRNLGANIHTEKNCWTVTGFGKHPRNPEKILDLQNSGTSFNLICGVS
                     CLGNFEVILDGDNSLRSRPVEPLLAALRNLGASALSINNNGKPPVKIKGPFSGGKTSV
                     SGINSQFVSSLLISTPLAPEDTEIAVNNLCEEPYVRMTLRWLDEQKIRYNKTEDLTHF
                     YVFGNQQYHPFEKVIPGDWSSATFPLVAGTITYSKISIKGLDINDTQGDKKVVEYLKK
                     FGACISVEKEQILVKNGLLKGIEIDLNSTPDALPAFAVLGCAAEGTTKLYNVPQARIK
                     ETDRIKVMAQELTKMGANIQEIEDGLIIHKSTLKGTVVNGHKDHRVVMALALAGLIAD
                     GETIITDAECVSITFPEFFSMMKNLGANVETENK"
     gene            4622..>4810
                     /locus_tag="PLW07_04305"
     CDS             4622..>4810
                     /locus_tag="PLW07_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49779.1"
                     /translation="MNTMTVSFSHAICPIYLDYSVEKLGNLFSRHKCGKKVLVITDEN
                     VAKLYADIVVNSISSSGKK"
BASE COUNT         1351 a          897 c          913 g         1649 t
ORIGIN      
        1 gattttttca aagattctct caaaacaatt tcccttccta tggtgttgac catttctatt
       61 tcagcgcttg cgagttcaat ttttgctggt aacaaaaaaa gatttttcac atgtgtttca
      121 agaattactt catctggatt aacttcgttg acaagaacat cataaatgga tttttcaagt
      181 ttgaagggtt caaaaccaag atggattgtt gtgtgtgcct gtggatcaag atcaatgagg
      241 agaaccctga atctcttttc cgtcactgcg gcaccaagat tgacaacagt tgttgttttt
      301 ccggaaccac ccttttgatt gattattgca attcttctca cattatctcc tatgaagttc
      361 ataacttttg atttttataa agtataaaat tttatagaat tttgtcaagt cctggatgtt
      421 atttctgata atttgattgt ttatttaagt tgtgcttttt ctctgtaaag gtgtagtcaa
      481 tatccgtttg ttaacacaat ttgcctgtat aaaataacac aactgagata tcaaacctga
      541 tttacatcac tataaatcct gctacaattg gtgtttcaat cacaatcact atatttattg
      601 atattagctt atgtttttga aattgcttaa cctggaaata aaatgtaaaa tttatacatg
      661 atcattacag gtgcgactat cattacgggt gtttttggat atccagtacg gcattccctt
      721 tcgcctgttt ttcaaaatgc tgccttccag tacagacatt taaactgggt atacgttcca
      781 ttcgagattt ctcctgaata tttgaaagat gctgtcaggg ctatcaagat tttttcagtc
      841 aaaggcataa acgttactat tcctcacaaa aaagaagtga ttaaatatct cgatgttctt
      901 gatgaagagg tgaaacttct tggtgttgca aataccattg taaacgaaga tggttgtttg
      961 aagggctatt ccactgatgg cgagggattt ctacgttcat taaaagaaga cggtggattt
     1021 actcctgaaa acaagttggt tttcctgttt ggttcagggg gttcatcttt tgctattgcg
     1081 ggtgctcttg tcagggcagg gatatcaggt ttgataatct gtaatcgcac aattgagaag
     1141 gcagcagttt tgaagaatca cctcaaagat tgttttagtt ttcagaatat tgaagtgata
     1201 ccctttgaga aaagagataa tgctggaatc tggaagaata tagaccttgt tgttaataca
     1261 acttctgttg gaatgaagga tgacgatata gtgctggtgg atgaaaagaa tttaaatgac
     1321 aatatgttca tttatgacat cgtctataac agagagacac ctttaattct gagtgcaaag
     1381 aaaaagggtg ttagatgtct tgacggtttg tccatgcttg tttttcaggg agcagtttct
     1441 ttcaccctct ggacaaaaat cgaagcaccc attgaagtga tgaaaaaatc tttgattgcg
     1501 tataaaaaaa ccatgcaaaa aggaaatcaa aattgaagat agtgcttgcc ggatttaatg
     1561 ggacatggag aaaagaagca ggaattatcc ttgcctcaag gctgggcagg aaattttttg
     1621 atatagaaga aatgatagag aaaagagaaa atgatagaat tgcacatatc tctcaaatta
     1681 aaggggctgc ttatgtgcga gatcttgaaa attcaattat tgaagaaatt tcagtttttg
     1741 ataattgcgt gatttctata ggacctgatg tcatagctaa tcagaaaaac cgtctatttt
     1801 ttaaatccaa tggtattgtg atatggttta cggcagaacc ttctgtgata ttgcttcgac
     1861 ttcatccggg aaaggaaagc aaaaacttat tgaaaagaca gaatgccatt tctcacatcc
     1921 gacagatatt aaaagagcat gattattcat gttttgctga taagataatt gacacttcat
     1981 cccttacttc tgaagaatcc gcagatagag ttcaggagat gttagcgctc ttttagtgtt
     2041 aaccatttct tatcaagtct ggcaagcgca tcataagctt ttgccatata aagggcgtca
     2101 agaatggcta ttgacatcat ggcttcggct acggcgtaga ttctgccgag tagagtaggg
     2161 tctcttcttg taatgggttt gagtttttct tctttcaatt ttttcatatt gatggaagtt
     2221 tgttctatag aaatagtcgg ggttggtttg actgcgattc ttactacgat atcttcgccg
     2281 tttgttattc caccaaggat accaccggca ttattggttc tgaatcttat ttttccgtgc
     2341 gaatctatat atggctggtc attattttct gatcctttca tattgccaac tctgaaaccc
     2401 gcgccaaatt caatcccttt aacagcacca atgctgcaga ttgcatgagc aataagtgca
     2461 ttgattttgt caaaaaccgg ctctccaagt cccggaggga cattatgggc tatcacctca
     2521 ataatgccac cagcagtgtc tccctggttt tttatttcca gcaccttttc ttccattttt
     2581 ttcgctgctt caaggtcagg acagtttatt tcattttttc tgtagttttt cttaatttcc
     2641 tcaaagggca tttcccttgc tcttattccc atgcactctt ttgtgtatgc cagaatttca
     2701 attccctctc gttcaagaat gaattttgcc acagctccac ctgcaaccct tgcaactgtt
     2761 tctctaccac ttgcccttcc tgcaccacac cagtcctgat attccccgta cttgaggaaa
     2821 aacggatatg ttgcatgccc gggtctaaaa atgtctttgt aagaacggta ctgttctaca
     2881 tgaatttttt ttgtgtcaac attatatatt atgataccga gcggagcacc tgttgttact
     2941 ccgtcctgac cgataccagc aaaaatttca cactgatctg tttcttttct tggactatta
     3001 agctttccga ttcctggttt gcgtttatca agttctttct ggatatattc tctcgttatt
     3061 ttgagccctg gtggcactcc gtcaactatc atggcgagag cggaaccttt ccctttacca
     3121 taagattctc cgcatgttgt gattctgaaa attcttccaa aagtattacc aagcataatg
     3181 cctcccttta tttgttttct gtttctacat ttgctccaag atttttcatc atagagaaaa
     3241 attcaggaaa agtgattgaa acacattctg catccgttat tattgtttca ccatctgcaa
     3301 tgaggcccgc cagggctaga gccataacaa ctctgtggtc cttgtggccg ttgacaactg
     3361 ttcctttaag tgtgcttttg tgaataataa gaccatcttc aatttcctga atgtttgcac
     3421 ccatttttgt cagttcctgt gccatgactt ttattctatc tgtttctttg attcttgcct
     3481 gtggaacgtt gtaaagtttt gttgttcctt cggcagcaca gccgagtaca gcgaatgctg
     3541 gcagggcatc aggcgtggaa tttaagtcaa tttctattcc tttcagcagg ccatttttta
     3601 ccagaatttg ttctttttct actgagatac atgctccaaa tttttttaaa tattcaacca
     3661 cttttttgtc accctgagta tcattgatat caaggccttt gattgaaatt tttgaatatg
     3721 taattgtgcc tgcaacaagg ggaaatgttg ctgaactcca atcgcctggt attacttttt
     3781 caaaaggatg atattgctgg tttccaaaaa cataaaaatg tgtcaaatct tctgttttgt
     3841 tatagcgtat tttctgttca tcaagccatc taagagtcat tctcacataa ggttcctcac
     3901 agagattgtt aactgcgatt tcggtgtctt caggagccaa tggagtagat ataagcaggg
     3961 atgatacaaa ctgggagttg attcctgaaa cacttgtttt tccacctgaa aaagggcctt
     4021 ttatcttcac gggaggtttt ccattgttat taattgataa tgcagatgcg cccagattcc
     4081 tcaacgcagc aagtaatggt tcaacaggtc tacttcttaa tgaattatca ccatccagga
     4141 tcacttcaaa attaccgagg caggatactc cgcaaataag gttgaatgaa gttcctgaat
     4201 tctggagatc taaaattttc tctggatttc tgggatgctt accaaatcct gttactgtcc
     4261 agcaattttt ttctgtgtgg atatttgctc caagatttct gcatgcatta acagctgcca
     4321 ttgtatccag agaatcaaga ggattgatga ttcttgaatt accagcggca agagatgcta
     4381 taaccactgc cctaatcgta tgagatttac tggcagggat taccgcgata ccattcaaac
     4441 attgtgtttt attaattttc agattcattt tgaacacctt tccttttatt atatcaattt
     4501 tttgtaatca tgaaaacccg ctaattgtgg tttgaggata gtcgtggact ttgaaacaca
     4561 caggtgacac cattaaaatc aaatgttata ataaattgaa aatgtacgaa ggaaggcata
     4621 aatgaacaca atgaccgtat ctttcagcca cgcaatatgt ccgatttatc ttgattattc
     4681 tgtggaaaaa cttggtaatt tgtttagcag acacaagtgt gggaaaaaag tgctggtcat
     4741 tactgatgaa aatgttgcaa aattgtatgc tgatattgtt gtcaacagca tttcttcttc
     4801 tggtaaaaaa
//
LOCUS       DAORYU010000102         4803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_12022_length_4803_cov_5.590143, whole genome shotgun sequence.
ACCESSION   DAORYU010000102 DAORYU010000000
VERSION     DAORYU010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12022_length_4803_cov_5.590143"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="PLW07_04310"
     CDS             <1..824
                     /locus_tag="PLW07_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOL49780.1"
                     /translation="TAPFGKQNLEAVLNFAKDNGFQKVEILSVPGAGHIAPDQVLADS
                     GKKLNELFHQTGVNPSSYACYTNVLDPDPKRRQLIKKHLENLILVAELTDVDVVCTIA
                     GMPVSGKTKMQTIEQDAAPYFQDLCSFAAKHRVKIALENWFETNIQHLDHWQKLFQLV
                     PSPNFGLNFDPSHLVHQGIDYLSAVDEFANRIFHTHAKDCEIKKHVLRRVGNREKGWW
                     RYVVPGFGVIRWGEYISRLRKAGYNGVLSIEHEDSAVGLEEGFIIGEKYLSQFIA"
     gene            772..2145
                     /locus_tag="PLW07_04315"
     CDS             772..2145
                     /locus_tag="PLW07_04315"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011929250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOL49781.1"
                     /translation="MKKVLSSGKSIYLNLLHKIDLPVQAYWVKIFAMDILIKNIRVID
                     PYTGTDGRRDVFISGHSYSAIGKNIHKRTSKIIDGTNLWLFPGLIDMHCHLREPGNEE
                     KETILSGSISAAAGGFTTVCCQANTEPAVDNKVVVGYIFEKAKKSPITVLPYGAVTVG
                     RKGAVLSPMGELSEAGVIGFSDDGNCVMDSLIFRRALEYSKTWGKIVVSHSEDQILAK
                     NGVMNEGVLSTKLGLRGIPAAAEHIMVYRDIELAKLTGARLHLAHLTTKKSVELVKEA
                     KKQSKNITCEVTAHHLTLTEEALSNYDANAKVSPPLRTQEDIRALIKGIKENIIDAIV
                     TDHAPHSEEEKGSGIENAPFGMIGFETALPLVMSLIKYGIKPIDILSKMTVGPAKILQ
                     LKENPIAEGSDATCVIFDPQKEWILTKENILSLSKNTPLLNKKLSGKVLCTLYRGNIV
                     YSEKTLL"
     gene            2648..>3357
                     /locus_tag="PLW07_04320"
     CDS             2648..>3357
                     /locus_tag="PLW07_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOL49782.1"
                     /translation="MKKGFTLIELLVVIAILGILAAMLLPALSKAREKARQSVCMSNF
                     KQIGIAMELYANDYDEFYPVNPYWKTRLWVYVSPEIRNKISQCPSRHGKTISPDNWFI
                     GQGYNIGSGIFPGFMGQKRSRIVNPSEKIMVLDWGREKDGKGGCNAGAPYSDNGVLSV
                     DSASAFNNAGTSHWAVCRIHSGGANILFGDGHVGWMKPEQFHSDVKDVDDNGNPIPAS
                     PNIAGEWRKYWDTGYSGS"
     assembly_gap    3358..3367
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3368..4178
                     /locus_tag="PLW07_04325"
     CDS             <3368..4178
                     /locus_tag="PLW07_04325"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HOL49783.1"
                     /translation="KKLFFSLALCVLSLFCYAVQIEIVATSSNIATTVKQIGQSKVNV
                     TVLIPPGICPGHYEIKPQDIKKLYKNGVLLYHGWEGFIDDISKATNGSGARIIQVDVN
                     GNWLIPEVQIHAAEKITEILSSTDPENKKFYEKNLVSYKNRMKTLDRKVKTMISTNKM
                     KDVPVICSAMQKDLIEYFGLKVIGCFGRDEEISPGVITNLINDANKYHVKIIVSNLQS
                     GTSVGEMLAKRMHLPHAVLSNFPGGLKGTDNVENTLLKNLQVIIDSLKTIQ"
     gene            4175..>4803
                     /locus_tag="PLW07_04330"
     CDS             4175..>4803
                     /locus_tag="PLW07_04330"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HOL49784.1"
                     /translation="MNNINFDCVSVARASRIVLHNISFTVQKGECVALYGPNGAGKTT
                     LLNLINGLLFAYKGKVSIGDIILTPKTALSVRLITGYVPQNFEVDTKIPILAKTVVLS
                     GCYGKIGLFNYPTQQHLNKTKKIMGLLEIEHIFDRPFGQISGGEKQKTLIARAMMQEP
                     EILLLDEPFSSISESSKWKILEIIKNWKKEKNITLFLVSHEKQIIEKLC"
BASE COUNT         1592 a          877 c          984 g         1340 t
ORIGIN      
        1 tgacagcgcc ttttggaaaa caaaatcttg aagcagtact caattttgca aaagacaatg
       61 gttttcaaaa ggttgaaatt ctctctgtgc ctggagcagg tcatattgct ccggaccagg
      121 ttcttgcaga ttcagggaaa aaactgaatg aacttttcca tcaaactggt gtcaaccctt
      181 caagttatgc atgttatacc aatgtgctgg accctgatcc gaaacgcagg caactgatta
      241 aaaaacatct tgaaaatctt atccttgtgg cagaattgac tgatgttgat gttgtctgca
      301 ccatagcagg catgcctgtt agtggaaaaa ctaagatgca aaccattgaa caggatgcag
      361 ccccttattt tcaagatttg tgcagttttg cagccaaaca cagggtaaaa attgctcttg
      421 aaaactggtt tgaaacaaat atacagcatc ttgaccactg gcaaaaactt tttcaactgg
      481 ttccctcccc aaattttggc cttaattttg acccatcaca tcttgtacat cagggaattg
      541 attatctttc tgcagtagat gaatttgcaa accgaatctt tcatacccac gcaaaggatt
      601 gtgagataaa aaaacatgtt ttgcgaaggg tcggaaacag agaaaaagga tggtggagat
      661 atgtagtacc aggttttggg gttattcgct ggggagagta tatttcacgc ttgagaaaag
      721 ccggttataa tggagttctc agtatagagc acgaagattc tgcagtgggt cttgaagaag
      781 gttttatcat cggggaaaag tatctatctc aatttattgc ataagataga tttgccagtt
      841 caggcatact gggttaaaat ttttgcgatg gacatactga taaaaaatat ccgtgtcatt
      901 gacccttata caggaacaga tggaagaagg gatgttttta tttcaggcca ctcttactcc
      961 gccattggaa aaaatattca caaaagaacc tcaaaaatca tagatggcac aaatttatgg
     1021 ctatttcccg gacttattga tatgcactgc catctcaggg agccaggaaa cgaagaaaaa
     1081 gaaaccattc tttcaggtag catctctgct gctgctggcg gttttaccac tgtgtgctgt
     1141 caggcaaata ccgaacccgc tgttgataat aaagtcgttg ttgggtatat ctttgaaaag
     1201 gcaaaaaaat cgccaataac ggtccttcct tatggagcag ttactgttgg cagaaaaggt
     1261 gcagtgcttt ctcctatggg tgaactgtct gaagcaggag tcattggatt ttctgacgat
     1321 ggaaactgcg tgatggacag tttgatattc agacgggcgc ttgaatacag caaaacatgg
     1381 gggaaaatag ttgtttctca cagcgaagac cagattcttg caaaaaacgg tgtgatgaat
     1441 gaaggtgttc tctccacaaa acttggttta agaggcatac ctgcagcagc agaacacatc
     1501 atggtttaca gagacattga acttgcaaaa ttgacaggcg cacgccttca tcttgcgcat
     1561 ctcacaacaa aaaaatccgt agaacttgta aaagaagcaa aaaagcaatc aaaaaatatt
     1621 acatgtgagg tcacagccca tcatttaacc ttaactgagg aagcactgag caattacgat
     1681 gccaatgcaa aagtcagtcc gccacttcgt acacaggaag acataagagc ccttatcaaa
     1741 ggaataaaag aaaacatcat cgatgccatt gtaacagacc atgctccgca ttctgaagaa
     1801 gaaaaaggtt caggaattga aaatgctcct tttggaatga taggttttga aacagcgcta
     1861 ccacttgtga tgtcgcttat aaaatatgga atcaagccca ttgacatcct ttcaaaaatg
     1921 acagttggac cagcaaagat attacaactg aaagaaaatc ctattgctga aggcagtgat
     1981 gcaacctgtg taatttttga cccacaaaaa gaatggatac tgacaaaaga aaatatttta
     2041 tccttgagca aaaatacgcc gcttcttaac aaaaaattat ctggcaaggt tctttgcacc
     2101 ctttaccgcg gtaatatcgt atattctgaa aaaaccctgc tatagttttt cacattagca
     2161 caagggggca gtacaatttt ctctcctgat ttcccccttt acaaaatttc cactctcatt
     2221 ctcccccttt gtaaaagggg gattaagggg gattttgttc tccctctata aaagggagat
     2281 gcaagaggga tttcagtaaa tcaatcccgg ccttcctttt gtaaaggaag gagacctgaa
     2341 gttggcggtg ggatttcaat aaatctctcc catcccctct tttataaaga ggggggtagt
     2401 atgaagtgat tttataaaga tgggaaacag tatgaggtga ttttgcaaag aggagtgtga
     2461 ttttgtactg ccccgtattt cagaccactt tattctacct tacttccaat tttttttaat
     2521 gggagcagta taatggtcat tatttttgtt gattttattt ttgagaaaaa ccgatctctt
     2581 gacatttcgt agcacatatt ttaaaattgt aacactaaaa atttttcctt atcacaggga
     2641 ggattgaatg aagaaaggtt ttacgctaat agaattgctg gtagtcattg cgattctcgg
     2701 cattctcgca gcaatgttac ttcctgctct gagcaaagca agggaaaaag caagacaatc
     2761 agtgtgtatg agcaacttca agcaaatcgg aatagcgatg gaactgtatg ccaatgacta
     2821 tgatgaattt tatcctgtca atccatactg gaaaacacgg ttatgggtat atgtgtctcc
     2881 tgaaataaga aataaaattt ctcaatgtcc atcaagacat ggaaaaacaa tcagtcctga
     2941 caactggttc ataggccagg gatacaacat tggtagtgga atttttcctg gtttcatggg
     3001 acaaaaaaga agcagaatag ttaatccatc tgaaaaaatc atggtgcttg actggggaag
     3061 agaaaaagac ggcaaaggtg gatgcaatgc aggcgcacca tacagtgata acggagtgtt
     3121 aagcgttgat agtgcttctg catttaataa tgcagggaca agccactggg ctgtatgtag
     3181 gattcattca ggtggagcaa atatcctttt tggagatggc catgtcggtt ggatgaaacc
     3241 agaacagttc cacagcgatg tgaaggatgt tgatgataat ggaaacccga ttccagcaag
     3301 tccgaatatt gctggtgaat ggagaaagta ctgggataca ggttattcag gctcctgnnn
     3361 nnnnnnngaa aaaattattt ttttctctcg cgctttgtgt tttatctcta ttctgctatg
     3421 ccgtgcagat agaaatcgtt gccaccagtt ccaatatcgc aacaactgtc aagcaaattg
     3481 gtcagtcaaa agttaatgtc actgtgttaa ttccacctgg tatttgtcct ggtcattatg
     3541 aaataaaacc acaggacatt aaaaaattgt ataaaaatgg agttttgctg tatcacggat
     3601 gggagggatt tattgatgac atttcaaaag ccaccaatgg ttctggcgca cgaataattc
     3661 aggtagatgt gaatggaaac tggctgattc ctgaagttca aattcatgct gcagagaaaa
     3721 taacagaaat actttcatcc actgaccctg aaaacaaaaa attttatgaa aaaaaccttg
     3781 tctcatataa aaaccgcatg aaaacacttg atagaaaagt aaaaacaatg atttccacaa
     3841 acaaaatgaa agatgttcct gtcatttgct ctgcgatgca aaaagacctt attgaatact
     3901 ttggattaaa ggtgatcggt tgttttggca gagacgaaga aatcagtcca ggtgtgatta
     3961 ccaatctcat caatgatgca aataaatatc atgtaaagat tattgtcagc aatctacaaa
     4021 gtggaacttc tgttggagaa atgctggcaa aaagaatgca tttacctcat gcggtattga
     4081 gcaattttcc tggtgggttg aaaggaactg acaatgttga aaacacgctt ttgaaaaacc
     4141 tccaggtcat cattgattca ttaaaaacca ttcaatgaac aatatcaatt ttgattgcgt
     4201 gtcagtggca agagcaagcc gcattgttct tcataatatc tcttttactg tacaaaaagg
     4261 agaatgtgtt gcactatacg gaccaaatgg cgcaggaaaa acaaccctat taaatcttat
     4321 caatggttta ttatttgctt ataaaggcaa ggtttcaatt ggcgatatca tactaacacc
     4381 caaaacagca ttatcagttc gacttattac tggatatgtc cctcaaaatt ttgaagtgga
     4441 tacaaaaatt cccattcttg caaaaacagt ggtattaagc ggctgttacg gcaaaatcgg
     4501 cctattcaat tatccaacac aacagcatct gaacaaaaca aaaaaaatta tgggactgct
     4561 tgagattgaa cacatttttg atagaccatt tggccagata tcagggggag aaaagcaaaa
     4621 aactttgatt gcaagggcaa tgatgcagga accagaaata ttgcttcttg atgaaccttt
     4681 ttcatctatc tctgaatcat caaaatggaa aattcttgaa atcataaaaa actggaaaaa
     4741 agaaaaaaac attacgctct ttcttgtttc tcacgaaaag caaataatag aaaaactatg
     4801 caa
//
LOCUS       DAORYU010000103         4766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_12134_length_4766_cov_3.982806, whole genome shotgun sequence.
ACCESSION   DAORYU010000103 DAORYU010000000
VERSION     DAORYU010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4766
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12134_length_4766_cov_3.982806"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="PLW07_04335"
     CDS             <1..399
                     /locus_tag="PLW07_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49785.1"
                     /translation="NKNVAYEPVNLARQGMRVSVRKRLLSRIEKMTGETGIRFEPDGR
                     VEFLIRNVDDGSVILYLANHEYKPANPILGLDLPKGKYTITMCGGDSFVLKQGLMNGI
                     YEPDSELFKNFTLQLAPREVLLIHLVRKQT"
     gene            468..>2591
                     /locus_tag="PLW07_04340"
     CDS             468..>2591
                     /locus_tag="PLW07_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49786.1"
                     /translation="MRKFFYICALCILFSGINNIYGESNLLKNPDFELIGKDGMPAFW
                     SREYNARLSGPFFVSNQAFEGKNAVCMLTEEWTLDRPQFIVQDVKLPPGVKRVRLSVF
                     AKGQGLFRLSIQFLDLKPKKVQEYFGEYSVAEETDRVFGLGPEYLKYTMEVSIPEGVN
                     NIRVRMGNTIGPLNLTNIWGKAWIDHADLTVFNDREQEQLSSLTITPASLQEKNAISK
                     KISVPSGFRDIAPYTLIVTDPPSYNTGNLVDGIEGIPGRQVSWKFEFLGGTERYPIIT
                     FFFPQPIPLKSVCLHLLGNVERFLVLANTDGTNKFNEVIADVKNVTGIDQWISLDLGE
                     KNISGLRIQAIEGRMQSYRSTMPFFDEAKILVREDYVKPILALLQKNSLYGKTYSRPN
                     VPSMFLEKTSIPVSKQQSVKRFRKMLTVDLWMFGIDLKKDTKVIDYGKTESFQKVIQT
                     CRQAGFDSLMLFLESYWKNLVPWPSKTASGTEENILKALAEAVHAEGLKLYVMPSSCL
                     NPPFSGKSLFVYPKEETSRYPQMEQFPDLIFGTYYRDKWLALLDEAMTCGCDGVSLCP
                     DETYYKGHFMETFPPDSPATLIYKQRYGKDLPEHEQDSLAYRQWITLRHESIADLYGY
                     WAKYLTSKYPDIYLSSRFMQPYLACSNITETGMPFDLLGSKGGIHELSSDYMGPYGIQ
                     MLAAANGWRKAGMCYDACMWGPLQGA"
     assembly_gap    2592..2601
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2602..3535
                     /locus_tag="PLW07_04345"
     CDS             <2602..3535
                     /locus_tag="PLW07_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49787.1"
                     /translation="VFWSMMYGLGMIDVYRQNYLVEQGTLPWFARAFNLLKDMEELGI
                     WEAHPPEKIAMFSSRASMDWWQVRAWWGNHNENWDRGIEGNRGWYADRVACNILQKKG
                     LPFDWFFLDNPGHLAKLKNYRVVVIPFAWSISRETAEQMKTAAKNGTRFILLDGKIGQ
                     TDQQGEPYEKPILQELVESGAATVIDDDIFTVGGTYAFEEKVSKIIEQILGNQIPLKF
                     QAYGKHVDATILEKNKNECFIFLINWEKAGLTPVDLGTSVSEGTYAVFVRDSDFWYQA
                     SINNKKQFTSADLAGFRFWMIPETACVLYLKKSE"
     gene            3556..4617
                     /locus_tag="PLW07_04350"
     CDS             3556..4617
                     /locus_tag="PLW07_04350"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOL49788.1"
                     /translation="MEEKAFEFLKSFLNIPSPSGFEQPAQKFFAEYVRDCVDKIEMDV
                     HSNVIATKNTEGKPKLMLAAHCDEIGLMVNYVTEDGFIYFVPIGGVSPYRLEGMRVFI
                     YGPKGRVPGVIARKPIGLRAENHQEKPARFQDLWIDIGARDKKDVLSVVNIGDPITIA
                     AEFQQLRNNCITARGLDDRLGVFVLAETMRRLKNEKIEPAVFAVSTVQEEVGSRGVWG
                     VAYRLKPDVAIVVEVEHASDHPDTEKQVMGEVFLGKGIGFNRGSNINHHLNTMLIETA
                     EKENVPYHITAVPGPTPTDASVIHIIREGVPCSVLRIPLRYMHTPSEIVSLDDVESAI
                     KLLASFIRRLDVSRKFIMM"
BASE COUNT         1441 a          937 c         1064 g         1314 t
ORIGIN      
        1 aacaagaatg tggcttatga acctgtcaat ctggcaagac agggaatgag agtttctgtt
       61 agaaaacgac ttctctcccg gattgaaaaa atgacagggg aaacaggtat caggtttgaa
      121 cccgacggca gggttgaatt cttaatcagg aatgttgacg atggaagcgt gatactttat
      181 cttgccaatc atgaatacaa acccgcaaat ccaattcttg gacttgacct gccgaagggt
      241 aaatatacaa ttaccatgtg tggtggggat agtttcgttc tgaagcaggg actgatgaac
      301 ggcatatatg aaccagattc tgaattgttc aagaatttta ctctgcaact tgctccaagg
      361 gaggtcttac tgatacacct tgtcaggaag cagacataaa atcagcaaaa aaataatcgg
      421 tgccatttca accgaactga aacagaacat tataacctgg aggaaaggtg agaaaatttt
      481 tttatatctg cgcactgtgc attttatttt caggtatcaa caatatctat ggcgagagca
      541 acctgttaaa aaaccctgat tttgaactga tcgggaaaga tggtatgcct gcgttctgga
      601 gcagggaata taatgctcgt ctttcaggac cattttttgt ttcaaaccag gcatttgaag
      661 gcaaaaacgc agtgtgcatg ctaactgaag aatggacgct tgacagacca cagttcattg
      721 tccaggatgt aaaacttcct ccaggtgtga aaagggtgcg attatctgtt tttgcaaaag
      781 gacagggact ttttcgcctg tccattcagt ttctggattt gaagccaaag aaagtacagg
      841 aatattttgg cgaatactct gttgctgaag aaacagacag ggtttttgga cttggacctg
      901 aatatctgaa atatacaatg gaagtgagca ttcccgaagg agttaacaat atcagggtca
      961 ggatgggaaa taccattgga cccctgaatc ttaccaatat ctggggcaag gcatggatag
     1021 accatgctga tttaactgtg ttcaacgata gagaacagga acaattgtct tctttaacaa
     1081 tcacacctgc ctcgcttcag gagaaaaacg ccatttcaaa aaaaatcagt gtaccatcag
     1141 gtttcaggga tattgcccct tatactctca ttgttactga ccctccctca tacaacacag
     1201 gaaaccttgt tgatggaatt gagggtattc ctggaaggca ggtttcatgg aaatttgaat
     1261 tccttggcgg aacagaacgt tatccaatca ttacattttt cttcccgcag ccaattccac
     1321 tgaaaagtgt ctgtcttcac ctgctgggca atgttgaaag atttcttgtc cttgcgaata
     1381 ctgatggaac aaacaaattc aatgaagtca ttgctgatgt aaagaatgta actggcatag
     1441 accagtggat atcccttgat ctcggcgaga aaaacatatc cggtttgaga atacaggcga
     1501 tagaaggcag aatgcagtct tatcgttcaa caatgccatt ttttgacgaa gcaaaaatcc
     1561 tggtcaggga agattatgta aaacccatcc ttgcgcttct tcagaaaaat tcattgtatg
     1621 gtaaaacata ttccagaccc aacgtgcctt caatgtttct ggaaaaaact tctatacctg
     1681 tttcaaaaca acaatcagta aaaagattca gaaaaatgtt gactgttgac ctctggatgt
     1741 ttggtattga ccttaaaaaa gatacaaaag ttattgatta tggaaaaact gaaagttttc
     1801 aaaaggttat tcagacatgc cgtcaggccg gttttgattc attaatgctg tttctggaat
     1861 catactggaa aaatcttgta ccctggccaa gcaaaacagc cagtggtaca gaagaaaata
     1921 ttttgaaggc gctcgctgaa gcagttcacg cagaaggtct gaaattatat gtgatgcctt
     1981 caagttgcct caatcctcct ttttccggaa aatccctttt tgtttatcca aaagaagaaa
     2041 caagcaggta tcctcaaatg gaacaatttc cagatttgat tttcggaaca tattaccgcg
     2101 ataaatggct tgctttgctg gacgaagcca tgacctgcgg atgtgatgga gtatctctgt
     2161 gccctgatga aacatactac aagggacact tcatggaaac gtttcctcct gatagcccgg
     2221 caacattgat ttacaaacaa aggtacggga aagatttacc cgagcatgaa caggactctc
     2281 tggcatacag acagtggatt actttgcggc acgaaagcat tgccgacctt tatggttact
     2341 gggcaaaata tctcacatca aaataccctg atatttattt aagttcccgt ttcatgcaac
     2401 catatctggc gtgctccaat attactgaaa caggcatgcc ttttgatttg ctcggtagta
     2461 agggtggcat tcacgaactc tccagtgatt acatgggacc atatggcatc cagatgctcg
     2521 ctgctgccaa tggatggaga aaagcaggta tgtgctatga tgcatgtatg tggggtccat
     2581 tacagggcgc gnnnnnnnnn nagttttctg gtcaatgatg tacggacttg gaatgattga
     2641 tgtctatcgt cagaattatc tggtcgaaca gggcactctt ccttggtttg ccagagcttt
     2701 taacctgttg aaggacatgg aagaacttgg catctgggaa gcgcatccgc cagaaaaaat
     2761 tgccatgttt tcttcaagag caagcatgga ctggtggcag gtcagggcat ggtggggaaa
     2821 ccataacgaa aactgggatc gtggcataga aggaaatcgt ggctggtatg ctgacagggt
     2881 cgcctgcaac attcttcaaa agaagggtct tccttttgac tggtttttcc ttgacaatcc
     2941 cggtcatctt gcaaaattaa agaattatcg cgttgttgtt atcccctttg cctggtcaat
     3001 tagcagggaa acagcagaac agatgaaaac tgctgcaaaa aacggtacaa ggtttattct
     3061 tcttgatgga aaaatcgggc agacagacca gcaaggagaa ccatatgaaa aacccattct
     3121 tcaggaactt gtggaatcag gagcagccac tgtcattgat gacgatattt ttacagtcgg
     3181 tggtacttat gcctttgaag aaaaagttag taaaatcatt gaacagatac tcgggaatca
     3241 aatccctttg aaatttcagg cctatggcaa gcatgtagat gcaacaattc ttgagaaaaa
     3301 taaaaacgaa tgttttattt ttctgattaa ctgggaaaaa gccgggctga ctcctgttga
     3361 cctgggtacc tctgtttctg aaggaacgta cgctgtcttt gtcagggact cagatttttg
     3421 gtaccaggct tccatcaaca acaaaaaaca gtttaccagt gcagaccttg caggttttcg
     3481 tttctggatg attccagaaa ctgcctgtgt tttgtatctg aaaaagtcag aataacattt
     3541 tttcgaggag gaagtatgga agaaaaagcg tttgaatttc taaaatcttt tctcaatatt
     3601 ccaagcccct ctggatttga acaaccagca cagaagtttt tcgctgagta tgtccgtgat
     3661 tgcgtggata aaattgaaat ggacgtccac agtaatgtca ttgccacaaa aaatacagaa
     3721 ggaaaaccaa aactgatgct cgcagcacat tgtgatgaaa ttgggctgat ggtcaattat
     3781 gttacagaag acggttttat ttactttgta cccattggtg gtgtttctcc atatcgactt
     3841 gaagggatgc gggtgtttat atatggtcca aaaggacgtg ttccaggagt gattgcaaga
     3901 aaaccgatcg gtttgcgggc tgaaaatcat caggaaaaac ccgcaagatt tcaggatttg
     3961 tggattgaca ttggagcaag agataaaaaa gatgttctct ctgttgtaaa cataggagac
     4021 ccaatcacaa ttgcggcgga atttcagcaa cttcgtaata attgcatcac tgctcgcgga
     4081 cttgatgaca gattaggtgt ctttgtactt gctgagacga tgaggcgact gaaaaatgaa
     4141 aaaattgaac ccgcagtttt cgcagtttcc actgttcagg aagaggtggg ctctcgcggg
     4201 gtatggggtg ttgcatatcg tctgaagcca gatgttgcca tcgttgttga agtggaacac
     4261 gccagcgatc accctgacac tgaaaaacag gtgatgggtg aagtgtttct gggaaaaggc
     4321 attggcttca atcggggttc aaacatcaat catcatctca acacaatgct gattgaaact
     4381 gccgaaaaag aaaatgtccc gtatcatatc acagcagttc ctggacccac tcccacagat
     4441 gccagtgtca tacacatcat aagagagggt gtaccctgca gcgtgttacg aataccatta
     4501 agatacatgc atacaccatc agaaattgtg tcccttgatg atgtggaatc agcgataaaa
     4561 ctacttgctt cttttattcg gcgactggat gtatccagaa aatttattat gatgtaggta
     4621 atccagaaaa catttactgt tcactggacg ttttcttccc gattctatcg tagaagagag
     4681 gccggggaaa tttttctgaa atccccttct aacttcaggt caccttccct tacaaaaccc
     4741 cattctactc ccctctttat aaagtt
//
LOCUS       DAORYU010000104         4753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_12174_length_4753_cov_4.170924, whole genome shotgun sequence.
ACCESSION   DAORYU010000104 DAORYU010000000
VERSION     DAORYU010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4753
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12174_length_4753_cov_4.170924"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1241
                     /locus_tag="PLW07_04355"
     CDS             <1..1241
                     /locus_tag="PLW07_04355"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOL49789.1"
                     /translation="IPLVKITKLADNIAMSLKASPIRMVAPIPGKSTIGIEVPNREIS
                     IVCLREILESKEFQNSPSKLTMAIGKNILGKPVISDLKIMPHLLIAGATGSGKTVCLN
                     SLITGILYKASPDEVKFLMIDPKRVELVIYNDIPHLILPVITDVKEAVTSLKWLIKEM
                     DRRYHLFAETKVRNIETYNQESEEKLPYIVVVVDELADLMLIARNEIENSIIRLTALS
                     RAAGIHLILATQRPSVNVITGIIKANLPCRIAFQVSSKFDSQTILDSKGAEKLLGRGD
                     LLFIPPGRSHPVRAQGGFVSDEELESMTDFLKAQGMPEYKMEILEHQPETQEGTVVTG
                     SSAGSDDDELYTRAVRIVLETRIASISMLQRRLEIGFNRAARLIERMEAEGIVGPYRE
                     GKPRVILKGVVPKDNEQTNP"
     gene            1222..1884
                     /locus_tag="PLW07_04360"
     CDS             1222..1884
                     /locus_tag="PLW07_04360"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HOL49790.1"
                     /translation="MSRPIHEILKEARLAAGIDLETVFEETFIPLKFLKSMENGEWEN
                     FPSRVHRDGFLKKYLAYLKLPEDIITECTEFRKEPEKEEEKEVTGREFSLVPFVIVLM
                     VVIFFVSAGIFLMRLSGRKSRNVQLPEKTSVQSVANEPVSITLKATEDVWLRVVSDGD
                     RIMERVLKPGENFSVTGKEIAIKLGNAGGLLIERNGKSYGPFGRSGQIMELNINASTR
                     FP"
     gene            1881..3176
                     /gene="rimO"
                     /locus_tag="PLW07_04365"
     CDS             1881..3176
                     /gene="rimO"
                     /locus_tag="PLW07_04365"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOL49791.1"
                     /translation="MKKLCLISLGCPKNTVDSEKIIGVLGDAGYEISLIPEESDFVVV
                     NTCAFIKPAINEARRVISTLKSRKKTSEFKLVVTGCLPARNYDELLGDKDIDAVIGPY
                     CIDRLPEIFSNIDSGERVYTHTDVHIHQYCRLPRAVSTLPYAYIKITEGCSNRCSYCT
                     IPFIRGPLYSFSEKDILKEAESLFVSGVKELIIVGHDITAYGKDRGKGNLCGLLRNIV
                     KIGFPWVRLMYLHPSGINQELLELMDQHSQICKYLDIPLQHVNPAILKKMNRPVKDYR
                     KMIEDIRRAIGDITLRTTLIVGFPGETEAIFQQLLDFIREMKFEKLGAFIYYPEKDTP
                     AFLMPDQIPVRVKEERYKRLMDVQKTISKDIAASFSGKTVEVLIEKQGKKYLSGRTRM
                     DAPDIDWTVKVYGQAKQGDIVNVRITKSSFYSLEGIVSE"
     gene            3353..4516
                     /gene="nusA"
                     /locus_tag="PLW07_04370"
     CDS             3353..4516
                     /gene="nusA"
                     /locus_tag="PLW07_04370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOL49792.1"
                     /translation="MKKELVALLDYWEKNKGVSKEFLISSLEEGLSAVYRKKALLEEN
                     ISVKIDPETGDINFIDEQGNQVPPPSFPWERIAAQTAKQILIQKIREAEKAAIYNEFK
                     KLENTIVTGRVERFETGNIVVAIGRTEGLLPQKHLLPHDRFHVGDPVRGLILEVRKPN
                     KGVYPIIMSRTATDVVRMLLMDEIPEIRDGLVVIKAIARFPGDLTKVAVDSSDSRIDP
                     VGTCIGDKASRIKNITKELGGEKIEVVRWYPDPEKFTASALLPAVCERVVFDRAKNEA
                     MVWVNREQLFVAIGKKGQNVRLACKLTGWNIIINRSEGEGPATLLLGISEELAERLSK
                     AGYETIKSLSEADNKNLSKKLNISEDEAKELIERAIRHMQDTQPDGKSNTSEN"
     gene            4516..>4753
                     /locus_tag="PLW07_04375"
     CDS             4516..>4753
                     /locus_tag="PLW07_04375"
                     /inference="COORDINATES: protein motif:HMM:NF016635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HOL49793.1"
                     /translation="MRVYQFAKQLKITAKELIEICENAGIKGKTYVSGLKDQEMAVIK
                     EYMKKSKLAGKPIVSTGTETVGEIASKLGISASEI"
BASE COUNT         1549 a          757 c         1118 g         1329 t
ORIGIN      
        1 gtatcccgct tgtgaaaatc acaaaactgg ctgacaacat tgctatgagt ttgaaagcaa
       61 gtcctataag aatggtagct ccaataccgg gcaagtcaac cataggtatt gaggtgccta
      121 acagagaaat ctccattgtg tgtttgagag aaattcttga aagcaaagag tttcagaatt
      181 ccccgtcaaa acttacgatg gcaataggaa aaaatattct tggcaaaccc gtgatatctg
      241 atttgaaaat catgccacat cttttgattg caggggcaac aggttcaggg aaaacagtat
      301 gcctcaattc acttattaca ggtattcttt ataaggcatc gcctgatgaa gtgaagtttt
      361 taatgattga cccgaaaagg gtggaacttg tcatttataa tgatatacca catctcattt
      421 tgccggtcat cactgatgtc aaggaggcag tcacttcgct taaatggtta attaaagaaa
      481 tggaccgacg ctatcatctt tttgcagaaa caaaggtgag aaacattgaa acatacaatc
      541 aggaatccga agaaaaactt ccgtatattg tggttgtggt tgacgaactt gcggatctga
      601 tgctgattgc aagaaacgag attgaaaaca gcatcataag attgacggct ctttcacgtg
      661 cagcaggaat acatttgatt cttgccacac agaggccatc tgttaatgtt attacaggaa
      721 tcatcaaggc gaatctgccg tgtagaatag catttcaggt atcttcaaaa tttgattctc
      781 agacaatact tgattcaaag ggagctgaaa aactacttgg aaggggagat cttcttttta
      841 ttccgcctgg aaggtctcat cctgtcaggg ctcagggcgg gtttgtcagc gatgaagaac
      901 tggaatcaat gacagatttt cttaaagcac agggtatgcc agagtataaa atggaaattc
      961 ttgaacatca gccggaaacc caggaaggta ccgttgttac tggttcttca gcgggtagtg
     1021 atgatgatga actttataca agggctgtga ggattgttct tgagacaaga attgcttcaa
     1081 tttccatgtt acagagaagg cttgaaatag ggtttaacag agcagcaaga ttgattgaac
     1141 gaatggaagc agagggtatc gtgggtccat acagggaagg taagccacgg gtaattctca
     1201 aaggtgtggt tccaaaagat aatgagcaga ccaatccatg agatattgaa agaagcccga
     1261 ttggcagcag gcatagatct tgagaccgtt tttgaagaga cctttattcc gctaaaattc
     1321 ttgaaaagca tggaaaacgg agaatgggaa aattttccgt caagggttca tagagatggt
     1381 tttttgaaaa aatatcttgc ataccttaaa ttacccgaag atattattac tgaatgcact
     1441 gagttcagaa aagaacccga aaaggaagaa gagaaagaag ttactgggcg tgaattttct
     1501 ttagttcctt ttgttatcgt tttgatggtg gtgatatttt ttgtttctgc gggaattttt
     1561 ttgatgagat tgtctggcag gaaatcaaga aatgttcaat taccagaaaa aaccagcgtt
     1621 caatctgttg ccaatgaacc tgtcagcatt accttaaagg caacagagga tgtctggtta
     1681 agggttgttt ctgatggcga caggataatg gaaagggtgt tgaaaccagg tgaaaatttt
     1741 tctgtgactg gcaaagaaat tgccataaaa cttggcaatg caggcggtct tttgattgaa
     1801 agaaacggga aaagttatgg gccttttgga agatcaggcc agataatgga gttgaatatc
     1861 aatgcgagca ccagatttcc atgaagaaac tttgcctgat aagtcttgga tgtcctaaaa
     1921 atacagtaga ttctgaaaaa atcatcgggg ttctcgggga tgctggttat gaaatttcat
     1981 taattcctga agaatctgat ttcgttgttg taaatacctg cgcatttatc aaaccagcaa
     2041 ttaatgaggc aaggcgggtg atttccacgc tgaaaagtag aaagaaaaca tctgaattca
     2101 aacttgttgt gactggttgt ttacctgcca gaaactatga tgagttgctt ggagacaaag
     2161 atattgatgc agttattggt ccgtattgta ttgacagatt acctgagatt ttttctaaca
     2221 ttgattctgg ggaaagggtt tatacgcata ctgatgttca tatacatcaa tattgtcgtc
     2281 tgccgagggc tgtttcaaca ttgccatacg catatatcaa aataacggaa ggatgcagca
     2341 accgatgttc gtattgcaca attccgttca tcagaggacc tctttatagt ttttcagaaa
     2401 aagatatttt gaaagaagca gaatctcttt ttgtttcagg agtgaaggaa ctgatcatag
     2461 ttggacatga tattactgcc tatggaaaag accgtggtaa aggtaacctg tgcggactgc
     2521 tcagaaatat agtaaaaata gggtttccgt gggtcaggtt aatgtatctg catccttcag
     2581 ggatcaatca ggaactattg gaattgatgg accagcattc gcaaatatgt aaatatcttg
     2641 atattccgct tcaacatgtt aatcctgcga tattgaaaaa gatgaatagg ccggtgaagg
     2701 attatagaaa gatgattgaa gatatcagac gggctattgg ggatattaca cttcgtacaa
     2761 cattgattgt gggttttccg ggtgaaacag aagcaatttt tcaacaactt cttgatttta
     2821 tcagagagat gaagtttgaa aaactcgggg ctttcattta ttacccggaa aaagataccc
     2881 cggcattttt gatgcctgac cagatacctg tcagagtaaa ggaagaaaga tataaaaggt
     2941 tgatggacgt tcagaaaaca attagcaaag atattgcagc atctttttca ggcaaaacgg
     3001 ttgaggtttt gattgaaaaa cagggtaaaa aatatctatc aggaagaaca aggatggatg
     3061 ctcctgatat tgactggact gtgaaagttt atggtcaggc gaaacaagga gatattgtta
     3121 atgtcaggat aacaaaaagt tctttttatt ctcttgaagg gatagtttcc gaatagattt
     3181 tcacatgaga tgatgttttt tgcattgcat tcagattcaa cttctttcat gattgtgatt
     3241 tgacactcca tcttattaca cttataatgt aatatcatga aaacatcctt cgatttctgc
     3301 aaaaatcttt gtatttttcg cgtaacaatg attgataaaa ggtgatgaaa aaatgaagaa
     3361 ggaacttgtg gcattacttg attactggga aaagaacaag ggtgtatcca aggaatttct
     3421 tatttcatcc cttgaagagg ggttgtctgc tgtttataga aaaaaagcac tgcttgaaga
     3481 aaacatcagt gtgaaaattg accccgaaac cggcgatatt aattttattg atgaacaggg
     3541 taatcaggtt cctccgcctt cttttccatg ggaaagaatt gcagcccaga cagcaaagca
     3601 gattcttatt cagaagattc gtgaggcaga aaaggctgcg atatacaatg agtttaagaa
     3661 acttgagaat accattgtta ccggtcgtgt tgaaaggttt gaaacaggaa atatcgttgt
     3721 tgctattgga aggactgaag gattgcttcc tcagaaacat ttacttccgc atgatagatt
     3781 tcacgtggga gaccctgtaa gggggttaat cctggaagtt cgcaaaccaa acaaaggtgt
     3841 gtatccaatt atcatgtcca gaactgcaac agatgttgtt agaatgttac tcatggacga
     3901 aattccagaa atcagagacg gcctggttgt tataaaggcg atagcaagat ttccaggaga
     3961 tctgacaaaa gttgctgtgg attcgtctga ttcgagaata gaccctgtcg ggacatgtat
     4021 aggagataaa gcaagcagaa taaaaaatat cacgaaggaa cttggtggag aaaaaataga
     4081 agttgttaga tggtatcctg acccggaaaa attcactgca agtgcccttc ttcccgctgt
     4141 gtgtgaacgt gtggtttttg acagggcaaa gaatgaagcg atggtctggg tgaacaggga
     4201 gcaattattt gttgctattg gaaagaaggg gcaaaatgtc agacttgcgt gcaaattgac
     4261 aggatggaat atcatcatta ataggtctga aggcgagggc cctgcaacgt tattgctggg
     4321 tatcagtgag gaactggcag aaagattgag taaagcagga tatgaaacaa ttaagtccct
     4381 gagtgaggcg gacaataaaa atctttccaa aaaattgaac atttcagaag atgaggcgaa
     4441 ggaacttatt gaaagggcta tcaggcatat gcaggataca cagcctgatg gcaaatcaaa
     4501 tacttcggag aactgatgag ggtttatcaa tttgcaaagc agttgaaaat aacggcaaaa
     4561 gaattgattg aaatttgtga aaatgccggg atcaagggca aaacctatgt gagtggcttg
     4621 aaagatcagg aaatggcagt tatcaaagaa tatatgaaga agagtaaact ggctggaaaa
     4681 cccattgttt caaccggcac agaaactgtt ggagaaattg cttcgaaatt aggtatttca
     4741 gcatcagaaa tta
//
LOCUS       DAORYU010000105         4686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_12404_length_4686_cov_4.332542, whole genome shotgun sequence.
ACCESSION   DAORYU010000105 DAORYU010000000
VERSION     DAORYU010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4686
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12404_length_4686_cov_4.332542"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PLW07_04380"
     CDS             <1..248
                     /locus_tag="PLW07_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HOL49794.1"
                     /translation="TGRSDYPNQINNRLAFPGIFRGALDARAKQITELMKKEAARAIA
                     KVIPENKLNPDYVIPSPFNKEVAYAVGEAVKKVARSR"
     gene            251..664
                     /locus_tag="PLW07_04385"
     CDS             251..664
                     /locus_tag="PLW07_04385"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOL49795.1"
                     /translation="MVFLIISIVLFSGAALLYRHANHLHFNRLSIIVSERISAVILMT
                     FYMVFFDTLNITAATTLLAFAGGVTIFISRLALLTSLKYGKISSSWTAVNLSVIIPLL
                     VSMIVWKETPNLRQTIGLLMVPLAIILLQEEPGEK"
     gene            668..1075
                     /locus_tag="PLW07_04390"
     CDS             668..1075
                     /locus_tag="PLW07_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49796.1"
                     /translation="MWMKYIMISFFLTGISDTTWKIAGEICGASANSYLLLFHIFALL
                     SAIVVLMKTRTKIKKNEFFLGSIIGFALIAGGIASMQAILRIPGIIFFPVSSCGNLLI
                     VTIMANILWKEKPTNRQIVGLVIACIAIVLITL"
     gene            complement(1065..1835)
                     /locus_tag="PLW07_04395"
     CDS             complement(1065..1835)
                     /locus_tag="PLW07_04395"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOL49797.1"
                     /translation="MFENIEQITFVSGFDGTEQTYLIRKPASFQYQGIVIFLHGATNH
                     QEQGLNRQIFNGTFGKLQDYFMGKNYVYVCPEYRGDSWMNQEAEYDLRFLIEQLTKDF
                     RTSRVLLMGGSMGGTSSLIFATRNPHLVSSCLALCPATDMKKLYYFWHGTERDFLAKT
                     IEIAYGGTPEKRSEQYKERSGIYFVEKLRSMPVAIIHGDMDMVIPVEHSREFVEKAKQ
                     TGVRIFYQEIKGGDHDSPIMDFTIVKKAIDWLEFVITE"
     gene            complement(1811..2557)
                     /locus_tag="PLW07_04400"
     CDS             complement(1811..2557)
                     /locus_tag="PLW07_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49798.1"
                     /translation="MLKFGLWDIVLIAVVAIQAGILAYVSDPRKKSLILVFPFPFTFA
                     TISLGGRIDATNVLGLINLAIFTYGVWFAYLKLKLNIVFSIVLFALLYCVLAMTVARI
                     LPDREILFWISVLGVVIFAILLALLTECPAENPYRTDLHPLIKIPIIVGVVSMLVILK
                     KQLAGFMTVFPMVGVIAAYEARFMLASVCRQMPVVMICLVSLMMTLKVVSSMTNFWVG
                     LAAGWVVFLLVLKTTGNFLWRQLCLKTLNR"
     gene            complement(2558..3364)
                     /locus_tag="PLW07_04405"
     CDS             complement(2558..3364)
                     /locus_tag="PLW07_04405"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOL49799.1"
                     /translation="MSTLCKKFLKNGKVEECPVYDMHAHMGSYCRIYFPYGDFDPVIT
                     KNRLDRAGIKLLIFCSHASLFSPDIGNKSNIDVVRKLPDRLRAYCGLNPNYPEIIEKD
                     VSEFEKYKDVFVGFKLLASYHRLPITHPNYAPAWQYADKHGLLVLLHTWGGDQFNGPE
                     QIRIVAEKYPGAKILMGHSCHGEWDKAVQLMKDFPNVYAELCAVVDDRGGLEKFIEGY
                     GSAKIVFGTDWPWFNHHYYIGAVLDSGISDQDAHNIFHLNAEKLLAPFIS"
     gene            complement(3361..4119)
                     /locus_tag="PLW07_04410"
     CDS             complement(3361..4119)
                     /locus_tag="PLW07_04410"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOL49800.1"
                     /translation="MNLEFFDSNVFIGRPMNGLFGAVSSADELLQEMEKQNIGKAIVW
                     HIFQYEGSPVEGNRILAEQIATSNKLYGCWTILPPQTEEVISKGFFERMKANHIVAIN
                     AFPEWHRFILDGVGFGKFLNEVSERKIPLFLTMGRTCISWESIHRLMRDFPNLTCVLC
                     DIGIWGVDRYTWPLLEKFQRFYIESSLLSLEEGGVEATVRRFGSEKIVFGTGFPVRYP
                     KAAILQLVHADISEKDKINIASKNLEKILQEEQL"
     gene            complement(4133..>4686)
                     /locus_tag="PLW07_04415"
     CDS             complement(4133..>4686)
                     /locus_tag="PLW07_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49801.1"
                     /translation="FRPYAGEFTIPIFLGLQFRASEIMGAIMNVQLSRLEGILKDLRR
                     IKKMFMEELSGVPGIGFARSNDIEGDCGVVVAFRFENEKQALDFAQSEGVVGWRPLDS
                     RKHVWFDWEPIINKRISGHPAMNPYHFPQNTYLRKDYTKEMYPKSIDNLGRTVFISVN
                     PDWSDEEIKVRISACKKAAEKLK"
BASE COUNT         1443 a         1030 c          793 g         1420 t
ORIGIN      
        1 gaacaggaag aagtgattac ccaaatcaaa tcaataatcg cctggcattt ccaggaattt
       61 tcaggggcgc tcttgatgca agggcaaaac aaatcactga attaatgaaa aaggaagctg
      121 caagagcaat tgctaaagta atcccggaaa ataaacttaa tcctgattat gtgataccat
      181 ctccattcaa taaagaagtg gcatatgctg ttggagaagc agtaaaaaaa gttgccagat
      241 ctcgataaag atggttttcc tgatcatcag tattgtctta ttttcaggcg ctgcgctttt
      301 atacaggcac gcaaatcacc tccattttaa caggttgtct attattgttt ctgaaagaat
      361 ctcagcagtg attcttatga cgttttatat ggtgtttttt gacactctca acattactgc
      421 tgccacaaca ttacttgctt ttgctggcgg agtcacgatt tttatttctc ggcttgcact
      481 gcttacttca ttaaagtatg gaaaaatatc aagttcctgg acagctgtaa atctgtctgt
      541 cattataccg cttcttgtgt caatgattgt ctggaaagaa acaccaaatc ttcgccagac
      601 aataggactt ttaatggtgc ctctggcaat tatacttttg caggaagaac caggagaaaa
      661 ataaaacatg tggatgaaat atattatgat cagttttttc cttacaggta tttcagacac
      721 aacatggaaa atcgcaggag aaatctgcgg tgcttctgcc aactcctatt tattactttt
      781 ccatattttt gccttactca gtgctatcgt tgtattaatg aaaaccagaa caaaaataaa
      841 aaaaaatgaa tttttccttg gctcaataat aggatttgct ctgattgcag gtggtattgc
      901 ttcaatgcag gcaatattga gaatcccggg tattattttt ttccctgttt ccagttgcgg
      961 caatctactt attgttacaa tcatggcaaa tatcctctgg aaggaaaaac caacaaatcg
     1021 gcagatagtg ggtcttgtga ttgcatgtat tgctattgta cttattactc tgtaataaca
     1081 aactccagcc agtcaattgc ctttttcaca atcgtaaaat ccattattgg tgaatcatgg
     1141 tcaccacctt ttatttcctg gtagaaaatt cttacacctg tctgcttggc tttttcaaca
     1201 aattccctgg aatgttcaac aggaatcacc atatccatat ctccatgaat aattgcgact
     1261 ggcattgacc gaagtttttc aacaaaataa atacctgacc tttctttata ttgttctgac
     1321 cttttttcag gagttccgcc ataagcaatt tctattgttt ttgccagaaa atccctttca
     1381 gtaccatgcc agaaataata aagttttttc atatctgtgg ctggacacag tgccagacac
     1441 gaggacacta gatgcggatt tcttgttgca aaaattaaac ttgatgtgcc gcccatagaa
     1501 ccacccatta ataaaaccct tgatgttctg aaatcttttg taagttgttc aatcaggaac
     1561 ctaagatcat attctgcttc ctggttcatc caggaatctc ccctgtattc aggacacaca
     1621 taaacgtagt tttttcccat gaaataatcc tgcaatttcc cgaatgtccc gttaaaaatt
     1681 tgcctgttca atccctgctc ctggtgattt gttgctccat gaaggaagat gacaattccc
     1741 tgatactgga aactggcagg ttttcgaatc agatatgttt gttctgtacc gtcaaaacct
     1801 gatacaaaag ttatctgttc aatgttttca aacataattg cctccacaaa aaatttcctg
     1861 tagttttcaa aactagcaaa aaaacaaccc agcccgccgc aagaccaacc cagaaattcg
     1921 tcatacttga aacaactttt aatgtcatca tcaatgacac aaggcatatc attaccactg
     1981 gcatttgtct gcatacgctg gcaagcataa atcttgcttc ataagcagca atcacaccta
     2041 ccattggaaa cactgtcata aaaccagcca gttgtttttt taatatcaca agcattgaga
     2101 caacaccaac aataatggga atttttatca aaggatgaag gtctgttcta tacggatttt
     2161 ctgcaggaca ctcagtcaat aaagccagca gtatcgcaaa tatcaccacg cccaacacag
     2221 atatccagaa caaaatttcc ctgtcaggaa gtatccttgc aactgtcatt gccagaacac
     2281 aataaagcag tgcaaacaac acaatggaaa aaacaatgtt cagtttcagt tttaaatagg
     2341 caaaccatac gccatatgta aaaatcgcca gattaatcaa ccctaaaaca tttgttgcat
     2401 ctatccttcc accaagagaa attgtagcaa acgtaaaagg gaatggaaaa acaagtatca
     2461 gggatttttt tcgagggtca gacacatatg ccagaatccc tgcctgaatt gcaacaactg
     2521 caataagaac aatatcccac agtccaaatt tcaacattca ggaaataaat ggcgcaagaa
     2581 gtttttctgc gttaagatga aaaatgttgt gagcatcctg gtctgaaatg ccagaatcaa
     2641 gcacagcacc aatgtaatag tgatggttaa accatggcca gtcagtgcca aaaacaatct
     2701 ttgcagagcc atatccttca ataaatttct caagacctcc cctgtcatca accacagcac
     2761 agagttcagc ataaacattt ggaaaatctt tcattaactg aactgcctta tcccattctc
     2821 catgacagga atgacccatc agtatctttg ctccaggata tttctcagca acaattctta
     2881 tttgttctgg accattaaat tgatcaccac cccaggtatg tagaagcacg agcaaaccat
     2941 gtttgtctgc gtattgccag gcaggagcat agttcggatg ggtaatcggt aatctgtgat
     3001 aactggcaag aagtttaaat ccaacaaaca catctttgta tttttcaaat tcactgacat
     3061 ctttttcaat gatttcagga taattcggat tcaatccgca atatgcacga aggcggtctg
     3121 gcagtttcct tactacatca atatttgatt tatttccaat atcaggagaa aaaagtgatg
     3181 cgtgagaaca aaaaatcaaa agttttatcc ctgccctgtc cagtcggttt tttgttatta
     3241 cagggtcaaa gtcgccatat ggaaaataaa ttctgcaata tgaacccata tgagcatgca
     3301 tatcataaac agggcattct tcaacctttc cgtttttaag aaatttttta cacaaggtac
     3361 tcatagttgt tcctcttgta aaattttttc aagatttttt gatgcaatat tgattttatc
     3421 cttttctgaa atatccgcat gcacaagttg taatattgct gcctttggat aacgaacagg
     3481 aaaaccagtc ccaaacacaa ttttttcaga accaaatctc ctgactgtcg cttcaacgcc
     3541 accttcttca agagaaagca aacttgattc aatataaaaa cgctgaaatt tttcaagaag
     3601 tggccaggtg tatctatcaa cgccccagat gccgatgtcg caaaggacac aggttaaatt
     3661 cggaaaatcc ctcatcaaac ggtgaattga ttcccatgaa atacaggttc tccccatcgt
     3721 caaaaaaagc gggatttttc tctcagacac ctcattaaga aattttccaa agccgacacc
     3781 atccagtata aacctgtgcc attctggaaa ggcattgata gcaacgatgt gatttgcttt
     3841 catcctttca aaaaaacctt tgcttattac ttcttctgtc tgaggtggaa ggattgtcca
     3901 gcatccatac agtttgttac ttgttgctat ctgttctgct aaaatacggt ttccttcaac
     3961 aggagaccct tcatactgga aaatgtgcca cacaattgcc tttccgatat tctgtttttc
     4021 catctcttgc aggagttcat ctgcactgct tacagcccca aacagtccat tcattggtct
     4081 tccaatgaag acatttgaat caaaaaactc aaggttcatt gaactctcca ttttatttaa
     4141 gtttttctgc tgccttttta cacgctgaaa ttcttacctt tatctcttcg tcagaccagt
     4201 cagggttgac gctaataaac acagttcttc caagattgtc gattgacttc ggatacattt
     4261 cctttgtgta atcctttctc aagtatgtgt tctgcgggaa atgatatgga ttcattgctg
     4321 ggtgtccact gatgcgctta ttaataattg gttcccagtc aaaccagaca tgctttctgc
     4381 tatcaagagg cctccatccg acaacgccct cactctgagc aaaatccagt gcctgctttt
     4441 cattttcaaa tcgaaaagcc acaacaaccc cgcagtcgcc ttctatatcg tttgacctgg
     4501 cgaaaccaat acccggaact cctgaaagtt cctccataaa cattttcttt attcttctca
     4561 aatccttgag tattccttca agtcggctca actgcacatt cataattgct cccataattt
     4621 cactggctcg gaattgcagt ccaaggaaaa tgggaattgt gaattcgcct gcatagggtc
     4681 tgaaag
//
LOCUS       DAORYU010000106         4678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_12426_length_4678_cov_5.223448, whole genome shotgun sequence.
ACCESSION   DAORYU010000106 DAORYU010000000
VERSION     DAORYU010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4678
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12426_length_4678_cov_5.223448"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /gene="hcp"
                     /locus_tag="PLW07_04420"
                     /gene_synonym="priS"
     CDS             <1..696
                     /gene="hcp"
                     /locus_tag="PLW07_04420"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015752802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOL49802.1"
                     /translation="QTYSERIFTTGLVGWPGIKHIPAKNGKKDFTPIIEAAKKAGGFE
                     KDVEGKDITVGFAHNTVLGIADKVLDAVKKGYIRRFFLVGGCDGAKPGRNYYTELAQS
                     IPQDCVILTLACGKFRFNTLDFGQINGIPRLLDCGQCNDAYSAIVIAKALADASGCSV
                     NELPLSLILSWYEQKAVCILLTLFSLGVKNTRLGPTLPAFVSENVLKVLIENFQVKPI
                     TTVENDLKEILGN"
     gene            751..1722
                     /locus_tag="PLW07_04425"
     CDS             751..1722
                     /locus_tag="PLW07_04425"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOL49803.1"
                     /translation="MGQQYHCGIGEHLIAQNAGVAMHQLHFDADAIVFAYKKAEKIAE
                     RIGIKSPVPHLAGFAYPHVASLGCEIFFPEDAEEPRPIPIIKTPSDINHLKEPDDYLS
                     APVIQKKLQTLKKLLEKVPSAPKSIGHLYEGPVTTAMLLMGESFLILPYDDPKRAHKL
                     LEFCAKSAVNYANTIRNKLGIPVEPCPVSIPDDFAGMFPPSLFEEFVLPYWEILYSGM
                     QASERYLHSELLRVEHLPLLEKANINLFDPSSDQYLTPEILKQHCPCEFTLLIKEWEI
                     AHLSIEELEKLYERYAESKPQSISFWLCFFEHEEKIIALLKKARSMS"
     gene            1738..3828
                     /locus_tag="PLW07_04430"
     CDS             1738..3828
                     /locus_tag="PLW07_04430"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HOL49804.1"
                     /translation="MNWWEKEPLRIIEICDAFDLQRLPVEVLAKTVKKLGGNVQHFHC
                     MELSTKEYGSGLDDRSLYFRTSAAKKQNPDRLGEYLPFAKKYGIRVVVYFNVHWYTIE
                     FGNRNPYWVQIKEDGNPVQEVYGTGTSFCINSPYREWVFQVLKDLCSYDIDGIFYDGP
                     IFFSNTCYCQYCQKLFEQQTGCCPVPKSDRNHHLWKEFVEFQAESMERFLEESNSIIK
                     TINPEILFYMNGNSSWPYWPTGRDNHRIIKHTDILGAEGGFLYGNLNQTPIFKPGLTG
                     KLLSSQAKEKVAVVFDCAGHKNWSWYLLPETEISILLYETSFSGAQWWVAVFPDDINQ
                     PEMKAISEFGNFIKKHPEPFVQTTSAAKIALLWPSKSAEHYSGSSVPLTDFTEEIKGH
                     EIGNLLMEFYGFYEALVKPKAIFDVIDEYNLDDIHNYELVILPNAACLSRHDCQQIRK
                     YVKNGGNIVASFETSLYDEHGKPGNNFELSDVFGVNFEGTIFGPMRHDYVSPANTENA
                     ILKNIIKKFFPAPEYGTNVKNISGKILLFYCEKLKGRYDHTPEISLKPMMVEHKYGKG
                     NSIYLAGTFGSTIANCRFREYFYLIENFCHLLSKPLLSFENEKNIEAFLRENKQDMFL
                     YIINLTGEQKRPLTYIEPIEKLRIKMTTKAKNVIALNSNVRIKPKKTPYGSEFALSHL
                     KNFEILKISKQEKP"
     gene            3825..4616
                     /locus_tag="PLW07_04435"
     CDS             3825..4616
                     /locus_tag="PLW07_04435"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HOL49805.1"
                     /translation="MKIYILTDLEGTAGVVDFESQAYQTGKYYEIAKELATEEINSAC
                     EGAFEAGANEIFVLDGHGPGGVIPEKIHPEAKLFHGRPLSKTFCLEKGWDAVFLIGYH
                     AMNGTENGNLNHTYNSRDIVKMTMNGKDIGEIGMEMYLAGHFNIPVILISGDEAACNE
                     ASTYNPDIEKAIVKWGITRTSAISLSPLKARNEIKEKSKTAVKRISEMKPVRMQGKCE
                     LIIEFISSTNAFVWQNKQGIETLNARTIKISGDNFIDVWKRFYLS"
BASE COUNT         1574 a          882 c          919 g         1303 t
ORIGIN      
        1 caaacctatt cagaaagaat ttttaccact ggtcttgttg gctggccagg catcaaacat
       61 attccagcaa aaaacggcaa aaaggatttt accccgatta ttgaggcagc caaaaaagca
      121 ggtggttttg aaaaggatgt ggagggtaaa gatatcacgg tgggatttgc acacaatact
      181 gtactcggga ttgcagacaa agttctggat gctgtaaaaa aaggatacat caggagattt
      241 ttcctggtcg gtggatgtga tggagcaaag ccaggaagaa actattatac agaacttgca
      301 caatccattc ctcaggattg cgtaatacta acactcgcct gcgggaaatt tcggtttaat
      361 acactggatt tcggtcagat taatggaatt ccacgacttc ttgattgtgg ccagtgcaat
      421 gacgcctatt cagccattgt tattgcaaaa gcccttgcag atgcttctgg atgttctgta
      481 aatgaacttc cactttccct tatcctttca tggtacgaac aaaaggcagt atgtattctt
      541 ctgacacttt tttctcttgg agtaaaaaat accaggctcg gaccaaccct acctgccttt
      601 gtatcagaaa atgtgctgaa agtactgata gaaaattttc aggtaaaacc aattactact
      661 gttgaaaatg acctgaaaga aattcttgga aactgaataa acttgacatt tcaatagcca
      721 cagtttatta ttccttgaca aaggaatcgt atgggacagc aatatcactg tggcattggg
      781 gaacacctga tagcacaaaa tgctggtgtt gccatgcatc aacttcattt cgacgctgat
      841 gctattgttt ttgcttataa aaaagcagaa aaaattgctg aaagaatcgg cataaaatca
      901 ccagtacctc atcttgcagg atttgcatac ccgcatgtag catcattggg ttgcgagata
      961 tttttccctg aagatgcaga agaaccaaga cccattccca tcattaaaac cccatcagac
     1021 attaaccacc tgaaagaacc agatgattat ctttcagcac ctgtgattca aaaaaaattg
     1081 caaacactta aaaaattgct tgaaaaagtt ccttctgcac caaaaagcat tggacacctt
     1141 tatgaaggac cagtaacaac agcaatgctt ctgatgggag agagttttct gatattacca
     1201 tatgatgacc caaaaagagc gcacaaactt cttgagtttt gcgcaaaatc tgctgttaac
     1261 tatgcaaata ccataagaaa taaacttggt attcctgttg aaccatgtcc tgtttctata
     1321 cctgatgatt ttgcaggtat gtttccgcct tctctttttg aagagtttgt gcttccatac
     1381 tgggaaattc tgtattctgg aatgcaggca agtgaaagat atcttcacag cgaactgctc
     1441 agggttgagc acctaccact tcttgaaaaa gcaaacatta atctttttga cccgagttca
     1501 gaccagtatc taactcctga gatattaaaa cagcattgtc cctgcgaatt tactctgctc
     1561 atcaaagaat gggaaatcgc acatctttct attgaagaac ttgaaaaact atatgaaaga
     1621 tatgctgaga gtaaaccaca aagtatcagt ttctggctct gtttttttga acatgaagaa
     1681 aaaattatcg cactgctgaa aaaagcaagg tcaatgtcct gaacactgtt atcatttatg
     1741 aactggtggg aaaaagaacc attaagaatc attgaaatat gcgatgcctt tgacctgcaa
     1801 agattacctg ttgaagttct tgcaaaaaca gtaaaaaaac ttggtggtaa tgtacagcat
     1861 ttccattgta tggaactttc tacaaaagaa tatggctcgg gccttgacga caggtctctt
     1921 tattttcgta cttctgcggc aaaaaaacaa aatccagata gacttggtga atatctaccc
     1981 tttgcaaaaa aatatggtat cagggtggtc gtttatttca atgttcactg gtatacgatt
     2041 gagttcggaa accgcaatcc gtactgggtt cagataaaag aagatggaaa cccagttcag
     2101 gaagtttatg gaacaggcac atcattctgt atcaatagtc cttacaggga atgggtgttt
     2161 caggttttaa aagacctgtg cagttatgat attgacggta tcttttacga tggtccaatc
     2221 tttttttcaa atacctgcta ttgtcaatat tgccagaaac tttttgagca gcagacagga
     2281 tgttgtcctg taccaaaatc tgacagaaat caccacctgt ggaaagaatt tgttgagttt
     2341 caggcagaaa gtatggaaag atttcttgag gaatcaaaca gcatcatcaa gacaatcaat
     2401 cctgaaatac tgttttacat gaatggaaac agtagctggc cttactggcc tacaggcaga
     2461 gataatcatc gcattatcaa gcacactgac attctgggtg cagaaggtgg atttctatac
     2521 ggaaatttaa atcaaacgcc aatttttaag ccaggactga caggaaaact tctgtcatca
     2581 caggcaaaag aaaaagtggc tgttgtattt gattgtgcag gacacaaaaa ctggagctgg
     2641 tatctacttc cagaaacaga aatatctatt ctgctttatg aaacaagttt ttctggtgct
     2701 cagtggtggg ttgcagtgtt tcctgatgac atcaatcaac cagaaatgaa agccatttca
     2761 gaattcggga attttatcaa aaaacatcct gaaccttttg ttcaaacaac atccgcagca
     2821 aaaattgctc tgttatggcc gtcaaagtca gccgagcatt atagtgggtc ttctgtgcct
     2881 ctcactgatt tcacagaaga aataaaagga catgaaattg gcaatctcct gatggaattc
     2941 tatggcttct atgaagcgct cgtaaaacca aaagcaatct ttgatgttat tgatgaatat
     3001 aatcttgatg acatacacaa ttatgaactt gtgatactgc ccaatgccgc gtgcctttcc
     3061 aggcacgatt gccagcaaat aaggaagtat gtaaaaaatg gcggaaatat cgtagcaagt
     3121 tttgaaactt ctctgtatga tgaacacgga aaaccaggaa ataattttga attatctgat
     3181 gttttcgggg ttaattttga aggtaccatt tttggaccca tgcggcacga ctatgtttcc
     3241 cctgcaaaca cggaaaatgc cattcttaaa aatatcataa aaaaattttt ccctgcccct
     3301 gaatacggaa caaatgtaaa aaatatcagt ggcaaaattt tactttttta ctgcgaaaaa
     3361 ttaaaaggca ggtatgacca taccccggaa atttccctga aacccatgat ggtggaacat
     3421 aaatatggaa agggaaattc aatttatctt gcgggtactt ttggttctac catagcaaac
     3481 tgcaggtttc gtgaatattt ttacctcata gaaaatttct gtcatcttct ttcaaaaccc
     3541 ctgttatcgt tcgaaaatga aaaaaatata gaggcctttc taagagaaaa taaacaagat
     3601 atgttcttat acattatcaa tctcactgga gaacagaaaa gaccccttac ctatattgaa
     3661 ccgatagaaa aattacgaat aaagatgacg acgaaagcaa aaaatgttat tgccctgaac
     3721 tcaaatgtca gaataaaacc gaaaaagacg ccctatggtt ctgaatttgc tctttcacat
     3781 ttaaaaaatt ttgaaatact aaaaatctca aaacaggaga aaccatgaaa atctacatac
     3841 tgactgacct tgaaggaact gcaggggttg ttgattttga atcccaggca taccaaacag
     3901 gcaagtatta tgaaattgca aaagaactcg caacagaaga aataaattct gcctgtgagg
     3961 gagcatttga agcaggagca aacgaaatct ttgttcttga tggacacggt ccaggcggcg
     4021 taatacctga aaaaattcat ccagaagcga aactgtttca cggtagaccc ctttcaaaaa
     4081 ccttttgtct tgaaaaaggg tgggatgctg tttttttgat tgggtatcat gcgatgaatg
     4141 gaactgagaa tggaaacttg aaccatacct ataacagcag agatatcgta aagatgacaa
     4201 tgaatggaaa ggatattgga gaaatcggga tggaaatgta ccttgcagga catttcaata
     4261 tccctgttat ccttatttca ggcgatgaag ccgcatgtaa tgaagccagt acttataatc
     4321 ctgatattga aaaagccatt gtaaaatggg gtatcacaag aacatctgca atcagtttat
     4381 caccactgaa agcaagaaat gaaatcaaag aaaaatcaaa aacagcagta aaaagaatca
     4441 gtgaaatgaa gccagtaagg atgcagggta aatgtgaact catcattgaa tttatttcat
     4501 ccacaaacgc ctttgtatgg cagaataaac agggtattga aacattaaat gccaggacaa
     4561 tcaaaatcag tggcgataat ttcattgatg tctggaagcg attctacctc agttgacatt
     4621 ttgattgggc gcacgtggat tcgaaccacg aacccctgcg ttatcagcac agtgctct
//
LOCUS       DAORYU010000107         4669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_12456_length_4669_cov_3.616385, whole genome shotgun sequence.
ACCESSION   DAORYU010000107 DAORYU010000000
VERSION     DAORYU010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4669
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12456_length_4669_cov_3.616385"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1129)
                     /locus_tag="PLW07_04440"
     CDS             complement(<1..1129)
                     /locus_tag="PLW07_04440"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOL49806.1"
                     /translation="MGEIFGFFLAAFITICFARPVAIILKIPTLIVYIIAGILFGPSG
                     FNLIRDISSLSYFYELGIILLMFSAGLELKIGHSKNHGGSLKVLYIFNMVIPGILGFL
                     YGLFTGKYLNTPGYIFIAFYMITLFSSPAVEVTTHIFREFSKKMNYKKTVFSRQLIFS
                     SVFADITSLFIFTGITAYYVLKDPYDFIKFIIFACSFFFVVLKGLPLLQEKILSKIKG
                     MNTSEAETTTLIMLVIMVVAIGAFLKIPAIACSFFAGISLANININRRVKNNIDFITL
                     SIFIPIVFIIVGARANLGIFNTTESFLLSFSTIMIFCLVRILCIYIPTRIQGFSVRDS
                     LGFGFSVIPQLTGTLAIAVISHDIGILPDILFNSVIILSIMT"
     gene            1229..1669
                     /locus_tag="PLW07_04445"
     CDS             1229..1669
                     /locus_tag="PLW07_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49807.1"
                     /translation="MKKLFLVVAGLVLVGTCMALAADQAAGKGATDRGRPGQEMKRGP
                     GEMGLGLYSQLNLTEEQKSKIQEIQKIRREQFAALKEDATLTQEQKREKMKTIMESTQ
                     KQMDEVLTPEQKQKLDQLREQMKQRWQERQKEGKGNKPSDKPAK"
     gene            complement(1707..2657)
                     /locus_tag="PLW07_04450"
     CDS             complement(1707..2657)
                     /locus_tag="PLW07_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002649017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="HOL49808.1"
                     /translation="MNLIDTIKGSLLENFFPAGWDLKKIDRCCSNPPEKILERQKWWN
                     KNFELVPCDSLEEFDIKMGHEIASEIADAHKQGRNLILILPVGPMGMYKWAVYFLKEW
                     NISCQHVYGFNMDEWSDADGNTLPPDNPGAFQNAMEHAFYGPLGKLTIPKNQRNFATK
                     KLLPSYPEKIEQLRKKGGKLVVIFGIGRVFHIAFWEPHFAAEFKTTDEWKRQPYRLGA
                     KLHPLTIEQNAITSFKSRTTLVPCFANTIGPGIFLKADRIIGGCDGSLGRGMMWQGMS
                     LWVTLRYGPDMWVPSSFMPTLPGRLFFLKELAGPLVAECN"
     gene            complement(2678..3655)
                     /locus_tag="PLW07_04455"
     CDS             complement(2678..3655)
                     /locus_tag="PLW07_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HOL49809.1"
                     /translation="MSVSFKELGLVNTREMFKKAMEGGYAVGAFNFNNMEQLQAIITA
                     CVEVKAPVILQVSKGARQYANQTMLRYMAQGAVQMAKEMGSNIPIALHLDHGDSYELC
                     VSCVENGFSSVMIDGSHLSYEENVKLTKKVVEYAHRFDVTVEGELGVLAGIEEHVSSE
                     KSHYTKPEEVQDFVSRTGVDSLAISIGTSHGAYKFKLKPGETVPPLRFDILEECEKRL
                     PGFPIVLHGASSVLPEYVDMINKYGGKIENAAGVPEEQLRRAAKSAVCKINIDSDGRL
                     VLTAMIRKYLAEHPSEFDPRKYLGPAREALKNMYADKCKNVLGCAGKAE"
     gene            complement(3724..>4669)
                     /gene="apgM"
                     /locus_tag="PLW07_04460"
     CDS             complement(3724..>4669)
                     /gene="apgM"
                     /locus_tag="PLW07_04460"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOL49810.1"
                     /translation="LDKNDVVFRCNTIFVDNGIIISHSAGHISSDESRHLIDVLNDKL
                     SDELIQFYHGFDYRHILVLKKIFSPEIECFPPHDFVGKKVNNLLPRSENRDAQKTVET
                     LKKLIEQAEQILNAHPVNLKRIKENKYPANFIWPWSPGKKPGMKPITKKFNMSGAVIS
                     AVDLIKGIGIYAGLDVINVKGATGLYNTNYEGKAEAAIEALKKYDLVYLHVEAPDEAS
                     HEGNLNLKIRCIEDFDKRIVGKIWKAVDLNNTVIAVIPDHFTPVSVRGHTNEKVPFLI
                     YSPDNHPDSIETFNEQTCNHGSLGVIKGEEFIRIVLQK"
BASE COUNT         1444 a          923 c          898 g         1404 t
ORIGIN      
        1 acgtcataat tgagagaata atcacagaat taaaaagaat gtcaggaaga attccaatat
       61 catgagatat tactgctatg gcaagtgtcc cggttaattg tggtattaca gaaaaaccaa
      121 aaccaagaga gtcccgcaca gaaaaaccct gaattcttgt tgggatataa atacaaagaa
      181 ttctcacaag acaaaaaatc attattgttg aaaaagatag caaaaaactt tccgtcgtat
      241 tgaagatacc aagatttgcc cttgctccga cgatgataaa tacaatggga atgaaaatac
      301 tcaaggtgat gaaatcaata ttgtttttta cccgtctgtt aatgttgatg ttagcgaggg
      361 aaattcctgc aaaaaatgaa catgcaatcg caggtatttt aaggaatgct ccaattgcca
      421 caaccataat cacaagcatt ataagtgttg tggtttctgc ttcagatgta ttcattccct
      481 tgattttgct gagtattttt tcctgtaaaa gaggtaatcc cttcagaaca acgaagaaaa
      541 aagaacaggc aaaaatgata aattttataa aatcgtatgg atccttaaga acatagtaag
      601 cagtgattcc tgtaaaaata aacaaacttg ttatgtctgc aaacacagaa ctgaagatta
      661 attgtcttga aaatactgtt ttcttataat tcattttttt tgagaactca cgaaatatat
      721 gtgtagtaac ctctactgca ggagatgaaa aaagagtgat catataaaat gctataaaaa
      781 tatagccagg tgtatttaag tattttccgg taaataaacc gtaaagaaat ccgagaatcc
      841 ctggtataac catattgaaa atatataaaa cctttaatga acctccatga ttttttgaat
      901 gaccgatttt caattcgagc cctgctgaaa acatcaggag gataattcca agctcataaa
      961 aataactgag agacgaaata tctcttatga gattgaatcc ggatgggcca aataatattc
     1021 cagcaataat gtagacaatc agtgttggaa tttttaaaat gattgcgaca ggtcttgcaa
     1081 aacatatcgt gataaaggca gccagaaaaa agccaaatat ttctcccata aataaattgt
     1141 aaattaaaaa catagtaatg gtcaacaatt taactaaacc aaatatgtgt tactgcgtca
     1201 aaaagttaaa cgaaaggagg tgaattcaat gaaaaaattg tttctcgtcg tcgcagggtt
     1261 ggttcttgtt ggaacctgta tggctcttgc tgctgaccag gccgctggaa aaggcgcaac
     1321 ggacagagga agacccgggc aggaaatgaa aaggggtccg ggagaaatgg gacttggctt
     1381 gtattctcaa ctgaatctta ctgaggagca aaaatcaaaa atccaggaaa ttcagaaaat
     1441 cagacgtgaa cagtttgctg ctttgaaaga ggatgccaca ttgactcagg agcagaaaag
     1501 agagaaaatg aaaaccatca tggagagcac acagaaacag atggacgagg ttttaactcc
     1561 tgagcagaag caaaaacttg atcagttgag agaacagatg aaacagagat ggcaggagcg
     1621 tcagaaagaa ggaaagggta ataaaccatc agacaagcct gcaaagtaaa gtcataagcg
     1681 gggaggattt ttcctccccg cctttttcag ttacattcag ccacaagtgg tcctgcgagt
     1741 tcctttaaaa agaacaatct gcctggtagt gttggcataa aactgcttgg aacccacata
     1801 tcaggtccat aacgaagggt aacccataga ctcattccct gccacatcat ccctcttccg
     1861 agtgaaccat cgcatccacc aattatccta tcagccttca gaaaaatgcc aggtccaatg
     1921 gtgttagcaa aacaggggac gagcgtggtt ctgcttttaa aactcgttat ggcgttctgt
     1981 tcgattgtca ggggatggag ttttgcacca agtcggtatg gttgcctttt ccattcatca
     2041 gtggttttga actctgctgc aaaatgtggt tcccagaaag caatatgaaa tacccttccg
     2101 atgccgaaaa ttaccacgag ttttccgccc ttttttctca gttgttcaat tttttcagga
     2161 tacgatggaa gtaatttttt tgtggcaaaa tttctttgat ttttcggtat tgttagtttt
     2221 ccgaggggtc cgtaaaatgc atgctccatg gcattttgaa acgctcctgg attgtctgga
     2281 ggaagagtgt taccatcagc atcgctccat tcgtccatat taaaaccata gacatgctga
     2341 catgaaatat tccattcttt cagaaaatat acggcccatt tatacatacc catcggacca
     2401 actggaagaa ttaaaatgag atttcttccc tgtttatggg catcagcaat ttcagacgct
     2461 atttcgtgtc ccatttttat atcaaattct tcaagggaat cgcagggaac aagttcaaaa
     2521 tttttgttcc accatttctg cctttcaaga attttctcag gcgggttcga acagcacctg
     2581 tcaatttttt taaggtccca tcccgcagga aaaaagtttt caagcaatga acctttgata
     2641 gtatcaatta ggttcatatt gcctccttgt tttagtttta ttctgcctta ccggcgcatc
     2701 ctaaaacatt tttgcattta tctgcataca tgtttttcaa cgcttctctt gcaggtccaa
     2761 gatattttct tgggtcaaat tcagatggat gctctgcaag gtacttgcga atcattgctg
     2821 tgagaaccaa ccttccatca gaatcaatgt tgattttgca aaccgctgat tttgccgcgc
     2881 gtcttaattg ttcttctgga acacctgcgg cattttctat tttgccacca tatttgttaa
     2941 tcatatctac atattctggt aaaactgatg atgccccgtg cagaacaatt ggaaaaccag
     3001 ggagtctttt ttcacattct tcgagaatgt caaacctcaa gggtggaaca gtttccccgg
     3061 gcttcaattt gaatttatag gcgccgtgtg aggtacctat tgagattgct aaactatcaa
     3121 caccagttct tgaaacaaaa tcctgaactt cttcaggttt tgtgtagtgg gatttttcag
     3181 aagaaacatg ctcttcgata cctgcaagaa ccccaagttc accctcgaca gtcacgtcaa
     3241 acctgtgcgc gtactcaaca actttttttg tgagtttaac attttcttcg taagaaaggt
     3301 gagaaccatc gatcattaca gaggaaaaac cgttttcaac gcaggataca cataactcgt
     3361 agctgtctcc atgatcaagg tgtagcgcta tggggatatt tgagcccatt tcttttgcca
     3421 tttgcacagc tccctgtgcc atgtatctga gcattgtctg atttgcatac tgacgtgctc
     3481 cttttgaaac ctgaagaatg acaggagctt ttacctcgac gcatgcagta ataatcgcct
     3541 gcaattgttc catattgttg aaattaaatg cgccgacggc ataaccgcct tccatcgctt
     3601 ttttgaacat ttcccttgta tttacaaggc ctaactcttt gaaactgaca gacatttttt
     3661 cctcctgttt gtggggttaa agagaaatta attttaacac aaattgaaaa agtgacaaaa
     3721 aatttatttc tggagaacaa ttcttataaa ctcttcgcct tttatgactc ccagactacc
     3781 atggttgcat gtttgttcgt taaatgtttc aatactatca ggatgattgt caggagaata
     3841 aataagaaat ggaacctttt cgttcgtatg acccctcaca gaaaccggag tgaaatggtc
     3901 tggaattacg gcaattacag tattgttaag gtcgaccgct ttccagattt ttcctacaat
     3961 tcttttgtca aaatcttcaa tacatctgat tttgagattc aggtttcctt catgagacgc
     4021 ttcatcaggt gcttccacat gcaggtatac aaggtcgtat ttcttaagag cctcaattgc
     4081 tgcttctgct ttaccctcat agttagtatt ataaaggcct gtggcaccct tcacattaat
     4141 aacatcaaga ccagcataaa ttccaatacc cttgataaga tcgacggctg aaatcactgc
     4201 tcctgacatg ttaaattttt ttgttatcgg tttcatgccg ggttttttgc caggagacca
     4261 tggccagata aaattcgcag gatatttgtt ttcttttatt cttttaaggt tgaccggatg
     4321 cgcgttcaat atctgttctg cctgttcaat aagttttttg agtgtttcta cggttttctg
     4381 cgcatcgcga ttctcactgc gcggcaaaag gttattcact ttcttcccca caaaatcatg
     4441 cggtggaaaa cattcaatct ctggtgaaaa tattttctta agaacgagga tatgtctgta
     4501 atcaaaacca tgataaaatt gaatcaactc gtcagaaagt ttgtcgttaa gcacatctat
     4561 cagatgcctg ctttcatctg atgaaatgtg cccggcagaa tggctaatga taattccgtt
     4621 gtccacaaag attgtgttgc aacggaacac aacatcattt ttatccagt
//
LOCUS       DAORYU010000108         4667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_12467_length_4667_cov_3.706418, whole genome shotgun sequence.
ACCESSION   DAORYU010000108 DAORYU010000000
VERSION     DAORYU010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12467_length_4667_cov_3.706418"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="PLW07_04465"
     CDS             complement(<1..384)
                     /locus_tag="PLW07_04465"
                     /inference="COORDINATES: protein motif:HMM:NF018621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1284 domain-containing protein"
                     /protein_id="HOL49811.1"
                     /translation="MTVVRPHHLFCILGFHGKGYNKKFVENMENICCKIKNDPNITLI
                     LVEGADDICSCCPHMRDSICVKKPDSEIFLKNAEKEIMKILRIDFNDSVEAKRIYNRI
                     KDFDFNDAIFKLCGECEWYTFGYCKS"
     gene            complement(387..989)
                     /locus_tag="PLW07_04470"
     CDS             complement(387..989)
                     /locus_tag="PLW07_04470"
                     /inference="COORDINATES: protein motif:HMM:NF014669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HOL49812.1"
                     /translation="MTYLTKKWNFVWQRYFDWICSATIIEKLVISLVFAFLTGVSAQI
                     TIKLPFTPVPITGQVLMVLLTGVFLGRSWAGISQGIYLIGGMTRIPWFAGGTGGLFNI
                     LSAPSFGYIVGFIPASLFVGYMVSRRNRVDKISSVICIMLGGVSIIYLFGALWFSLMM
                     KTGFKQTLLLSVIPFVPFDILKAYIAALFTMALVSRPEIK"
     gene            complement(1001..2077)
                     /locus_tag="PLW07_04475"
     CDS             complement(1001..2077)
                     /locus_tag="PLW07_04475"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOL49813.1"
                     /translation="MGKGKIYKVGVIPGDGIGPEIINAGMEVLKSAAGRFNFKLDTVY
                     YDFGGDRYLKTGETLPDSAIEEFKKLDVIFLGAIGHPDVQPGILEKGILLKIRFELDQ
                     YINLRPVKLYPGVWTPLKDKGPEHIDYVVVRENTEGAYCGIGGSFKKGTKDEIATQLD
                     ISTRKGVERCVRYAFELARKRNKNKTLTCVDKANVLTFSGDLWRRTCQEVQKEYTDIK
                     LDFAFVDATCMWMVKNPEWFDVIVTNNMFGDIITDLAAMTQGGLGVAAGGNINPEGVS
                     MFEPIHGSAPKYTGKNVICPIATIAAGAMLLDTLGETEAAETIEKAVCKALSEGHVKS
                     LEAGKMGMGTAEVGALIAGFVSEI"
     gene            complement(2050..2541)
                     /locus_tag="PLW07_04480"
     CDS             complement(2050..2541)
                     /locus_tag="PLW07_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013748807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOL49814.1"
                     /translation="MIIKGKVWKFGDHINTDDIIAARYLNTTDVRELAAHCMETIAPD
                     FSKKIAKDDIIVAGENFGCGSSREHAPLAIKGCGISVVVAKSFARIFFRNAINIGLPI
                     IEIPFADKISQGDILEVNFDSGIIVNLTNGEKGTFMKYPEFLQQIIKSGGLMSWVKEK
                     YIR"
     gene            complement(2538..3800)
                     /gene="leuC"
                     /locus_tag="PLW07_04485"
     CDS             complement(2538..3800)
                     /gene="leuC"
                     /locus_tag="PLW07_04485"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOL49815.1"
                     /translation="MSMTITEKILSVHAGLKEVIPGQFIEANVDLVLANDITGPLAIE
                     EFTKLGMNKVFDPTKIVLVPDHFTPCKDIRSAEMVKVLRNFAKKYSVRFYEIGKVGIE
                     HALLPEQGLISAGDLIIGADSHTCTYGALGAFSTGVGSTDVAFAMITGKIWLKVPPTI
                     KFVYKGRCGKYVGGKDIILYTIGKIGVDGARYRAMEFTGEAIDGLSMDDRLTICNMAI
                     EAGGKNGIIAPDSITEEFLLTSGANTGTFYRSDEDAEYENIIEIDAGAIEPQVSAPHL
                     PSNAKNVREFSSVSIDQVVIGSCTNGRISDLRKAAMIAKGRSVHPEVRTIIIPATRKV
                     YQQALREGLIDIFVESGCVISPPTCGPCLGGHMGVLGKGEVAIATTNRNFIGRMGHPE
                     SMVYLSNPEVAMASAILGKISHPDEVQK"
     gene            complement(3832..>4667)
                     /locus_tag="PLW07_04490"
     CDS             complement(3832..>4667)
                     /locus_tag="PLW07_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HOL49816.1"
                     /translation="GNASLEEIAMNLIVRKKFYGVDVDLDTKHIYRISKLVSSLTGIP
                     VQPNKAIVGDNAFKHESGIHQDGVIKCRETYEIMHPKMVGIPESTLVLGKHSGRHALK
                     LRLESLGVSVDEKTLNKIFEQFKTLADKKKEVNDLDLIVLAEQETLSAVEPVYTLEYF
                     HIVSGTSTLPSATVRIRKKDEILEDASLGDGPIDALYRAIDRIVKISPKLHEYKIAAV
                     TGGKDAQGEVIVSLIIDGVRVAGKGVSTDIIEASARAYLNAINQYLTKKTIIKKKYRG
                     T"
BASE COUNT         1409 a         1081 c          710 g         1467 t
ORIGIN      
        1 agatttacaa taaccaaatg tataccattc acactctcca cataatttga aaatagcatc
       61 attaaaatca aaatctttta tcctgttata aattcttttt gcttcaacag aatcattaaa
      121 atcaattctt aatattttca taatttcttt ttctgcattt tttaaaaata tttctgaatc
      181 tggtttcttt acacaaattg aatcacgcat atggggacaa caactacaaa tatcgtctgc
      241 accttctacc aggattagtg taatattcgg atcattttta atcttgcaac agatattttc
      301 catgttctca acaaattttt tgttataacc cttgccgtga aatccaagaa tacagaaaag
      361 atggtgaggt ctgacaacag tcatagctat tttatttcag gtctggaaac aagtgccatc
      421 gtaaataaag cggcaatgta ggctttcaaa atatcaaatg gtacaaaagg aattacagaa
      481 agtaataacg tctgtttaaa tcctgttttc atcatcagcg aaaaccataa agctccaaat
      541 aaataaataa tagaaacacc accaagcatt atgcaaatca cacttgagat tttatcaacc
      601 ctgttccttc tgctgaccat ataaccaaca aacaaagaag caggaataaa accaacaata
      661 taaccaaatg acggagcaga caaaatatta aacaatccac cagttccgcc ggcaaaccag
      721 ggtattcgtg tcattccacc aattagataa attccctgac tgatacctgc ccagcttctg
      781 cctaaaaata cacctgttaa aagcaccatc aatacctgcc cggttatagg gacaggagta
      841 aaaggtagtt ttattgtaat ttgagcagaa acacctgtga ggaaagcaaa aactaaagaa
      901 atcaccaatt tttctatgat tgttgctgaa catatccagt caaaatatcg ctgccagaca
      961 aaattccatt tttttgttaa atatgtcata gccatatcct ttaaatttca gaaacaaatc
     1021 cagcaatcaa tgcgccaacc tcagcagtac ccattcccat tttgcctgct tcaagactct
     1081 tcacatgacc ttctgaaagc gccttacaaa ctgctttctc tatggtttcc gcagcctctg
     1141 tttctccaag agtatcaaga agcatagcac cagctgctat cgtagctatc ggacagatta
     1201 catttttccc tgtatatttt ggtgcagaac catgtattgg ttcaaacata ctcactcctt
     1261 caggattgat attacctcct gctgcaactc caagacctcc ctgcgtcatg gcagcaaggt
     1321 ctgtgatgat gtcaccaaac atgttgtttg taacaatgac atcaaaccac tcagggtttt
     1381 ttaccatcca catgcatgtt gcgtcaacaa atgcaaaatc cagttttata tccgtatatt
     1441 ctttttgcac ttcctgacag gtacgcctcc ataaatcccc agaaaaggtt aacacattgg
     1501 ctttgtcaac acaggtcaat gttttgtttt tatttctttt tctggcaagt tcaaaggcat
     1561 atctgacgca cctttccaca ccctttcttg tgctgatgtc aagctgtgta gcaatttcat
     1621 ccttggtccc tttcttaaac gaacctccta ttccacagta cgctccctct gtattttccc
     1681 gtacaacaac atagtcaata tgttcgggcc ctttatcttt caagggtgtc cacacacctg
     1741 gatataactt cactgggcgt aagtttatat actggtcaag ttcaaacctg atttttaaca
     1801 atattccttt ttcaagtata cctggttgca catcaggatg accgatggct ccaagaaata
     1861 tcacatcgag ttttttaaat tcttctatcg ctgaatcagg cagggtttca cctgttttaa
     1921 gatacctatc tccaccaaaa tcatagtaca ctgtatccag tttaaaatta aatcgaccgg
     1981 cggctgactt caaaacttcc attccggcat taatgatttc tggcccaata ccatcacctg
     2041 gaataactcc taccttatat atttttcctt tacccatgac attaatcctc cacttttgat
     2101 gatttgttga agaaattcag gatacttcat aaaagttcct ttttctccat ttgttaaatt
     2161 tacaatgatc ccactatcaa aattaacttc taaaatatca ccttgcgaaa ttttatcagc
     2221 gaatggtatc tcaataatcg gcaggccgat atttatagca tttctaaaga atatcctggc
     2281 aaaacttttt gcgacaacaa cagaaatacc gcatcctttt attgcaagcg gtgcatgttc
     2341 acgacttgaa ccgcagccaa aattttctcc agcaacgata atatcatctt ttgctatttt
     2401 ttttgaaaaa tcaggagcaa tggtttccat acaatgagca gccaattctc taacgtcagt
     2461 agtattaaga taccgggctg ctataatatc atctgtgtta atgtggtcac caaatttcca
     2521 tacttttccc ttaataatca tttttgcacc tcatcaggat gtgaaatttt acccagaatt
     2581 gcagacgcca tagccacctc tggatttgaa agatatacca tgctttccgg atgtcccatt
     2641 cttccaataa aatttctgtt cgtggttgca attgctactt cacctttccc gaggacaccc
     2701 atatgtccgc ctaaacacgg accacaggtt ggaggactta tcacgcagcc agattcaaca
     2761 aaaatatcaa ttaaaccttc tcgaagcgcc tgctggtaaa cctttcgtgt tgctggaata
     2821 ataattgttc tcacttctgg atgaactgac ctgccttttg caatcatcgc ggcttttcta
     2881 agatcagaaa ttcgtccgtt tgtacatgaa ccaatgacaa cctgatctat gctcacactt
     2941 gaaaattctc tcacattttt tgcatttgaa ggtaaatgag gagctgaaac ctgtggttca
     3001 atagcaccgg catctatttc aataatgttt tcatattcag catcttcatc acttctataa
     3061 aaagtcccgg tattagcgcc cgaagtcaat agaaattctt cagtaatact atccggtgca
     3121 ataatcccat ttttcccacc tgcttcaatc gccatattac aaatggtcaa tctatcatcc
     3181 attgaaagac catcaatcgc ttctccggta aactccatgg ctctgtatct ggcaccatcc
     3241 acacctattt tcccaatggt ataaagaata atatcttttc ctcctacata tttaccgcat
     3301 ctgcctttat aaacaaattt gattgttggc ggaactttta accagatttt gccggtaatc
     3361 atagcaaacg caacatctgt acttcctaca ccagtagaaa aagctccaag agcgccataa
     3421 gtacaggtat gactatctgc gccaatgatg agatctcctg cagaaatgag tccctgttct
     3481 ggaagaagcg catgttcaat acctaccttg ccaatttcat aaaatcttac actatacttc
     3541 tttgcaaaat ttctaagaac cttcaccatt tcagcgcttc ttatgtcctt gcatggagta
     3601 aagtgatcag gaacaagaac aatcttcgtc ggatcaaaaa ctttgttcat ccccaactta
     3661 gtaaattcct caattgcaag tggaccagta atgtcatttg cgagaacaag atcaacattt
     3721 gcttcaataa attgacctgg aataacttcc tttaatcctg catgaaccga taatattttt
     3781 tcagtaattg tcattgaaat cctttcggta tcaatatttt aagaaattaa tttacgtacc
     3841 cctgtatttt ttcttaataa tggtcttttt ggttaaatac tggtttatcg cattgagata
     3901 agccctggca ctcgcctcaa taatatcagt gctcacacct tttccggcga ccctcacacc
     3961 atcaataata agggaaacaa tcacctcacc ctgagcatcc tttccacctg ttactgctgc
     4021 aatcttatac tcatgcaact tcggcgatat tttaactatc ctgtcaatcg ccctgtaaag
     4081 ggcatcaata ggaccatcgc caagagaagc atcttccaga atctcatcct ttttacgaat
     4141 tcttactgtg gcgcttggta atgtggaagt ccctgagacg atgtgaaaat actccagggt
     4201 ataaaccggt tcaacagcag atagcgtctc ctgttcagca agaacaatca agtcgagatc
     4261 atttacttct tttttcttat ctgcaagagt tttgaactgt tcaaaaattt tattcagtgt
     4321 tttttcgtca actgaaacac caagagattc aagtctcagt ttcaaagcat gcctgccaga
     4381 atgctttcca agaacaaggg tactttcagg aattcctacc attttgggat gcataatttc
     4441 ataggtttcc ctgcatttta ttacaccatc ctgatgaatt cctgattcgt gtttgaatgc
     4501 attatcccca actattgcct tgtttggctg cacaggaatg ccggttagag aagacacaag
     4561 cttgctgatc ctataaatgt gttttgtgtc aaggtcaaca tctaccccgt aaaatttttt
     4621 ccttacgata agattcatag ctatttcttc aagcgatgca ttccctg
//
LOCUS       DAORYU010000109         4661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_12489_length_4661_cov_4.481329, whole genome shotgun sequence.
ACCESSION   DAORYU010000109 DAORYU010000000
VERSION     DAORYU010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4661
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12489_length_4661_cov_4.481329"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="PLW07_04495"
     CDS             <1..553
                     /locus_tag="PLW07_04495"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOL49817.1"
                     /translation="KIQHACVHWQTGRLGNIGTHIFDALRMLTGQQPVAVSGSLDPTL
                     YPDCRGLQYKDYGGWGIIIFSDGMRAFIDAPQTGLFPLVVKITGDRGEIIIQNKEAGI
                     HFWQGGSQEILPEPGNKTALDNAMEDIVSCLVESKVPACTGKDGLIALEVIIGFHVSH
                     RLGGKWIDLPIKGNDRNFEVMMA"
     gene            580..1662
                     /locus_tag="PLW07_04500"
     CDS             580..1662
                     /locus_tag="PLW07_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49818.1"
                     /translation="MKLKIFSVFVVFFLCGLLFGSPENTFSIVNPYDGIAWEKVVQYK
                     ANLHTHTTVSDGKLHPQHIVDIYASLGYKILSITDHSEMNKNPGNITYPWENFRNLVT
                     SGGKSGLYENRDSKLMGMLAVPGREISQEHDIGSYFNVSIDINSAKKIEDVIESIEKN
                     QGLAVLLHPGRYYFTDDWYVNLLKKYPVIVGMEVYNQGDRYPGCKIIWDNVLTKLMPD
                     RPVWGFSNDDAHRFEHIGKNWNVFLLSNLTIEELKHAMEKGQFYFAYCPDGINSTPAI
                     IRKIEVDNKNMLIHIEAINCSKIEWISNGKIVGTGPEYQIKTSDGKVANYVRAKLYGS
                     DNKSVTELQPFGIQKKRLIEMQAKLD"
     gene            1721..3001
                     /gene="eno"
                     /locus_tag="PLW07_04505"
     CDS             1721..3001
                     /gene="eno"
                     /locus_tag="PLW07_04505"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOL49819.1"
                     /translation="MKVVISSLSAIEILDSRGNPTIRVFCCLSNGIKVSSSVPSGAST
                     GENEAVELRDGDKKRYGGKGVLKAVENVNNIIAPKIIGIEPHRQAEIDQIMIELDGTP
                     NKGHLGANAILGVSMAVAKAQAKLSGLPLYQYLGGTGATRLPVPMMNILNGGKHADNS
                     VDFQEFMVMPVGAPNFKEALRYGAETFHALKKILSEKGYSTGVGDEGGFAPNLSSNDE
                     ACEVIVKAIEKAGYKPGIDIAIALDPAASSFYEDGIYNLSKSKQGKKTSEEMIELYGN
                     WIKKYPIVSIEDGLAENDWDGFKKMTEKLGNKIQIVGDDLYVTNTKFVEKGIKEKASN
                     AVLIKLNQIGTVTETIQTINLCRKAGWGYVISHRSGETEDTFMADFSVAMAGGQIKTG
                     SACRSERIAKYNRLLEIEWELGKSAVFSNPFQRD"
     gene            3014..3442
                     /locus_tag="PLW07_04510"
     CDS             3014..3442
                     /locus_tag="PLW07_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49820.1"
                     /translation="MIFKLSANHKRALETALTMLDELLVSIEKWVDGNQYQSVLYEEK
                     NDLSVSQRKALKKQILVLKASLEAAKKDLGLTKTTQSALNDIWSRVASFRENVMELGA
                     RHLKRYGSLHHETAEYMDKLSEKLLLQLDGILEIIKAGRH"
     gene            complement(3439..>4661)
                     /locus_tag="PLW07_04515"
     CDS             complement(3439..>4661)
                     /locus_tag="PLW07_04515"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HOL49821.1"
                     /translation="SKRKNILTNFNLSVAVTDKFMESVFKNQKFELVNPRTKTAVKSI
                     PARELFDIISQCAWETGDPGIIFIDEINRKNPTPEIGSIEATNPCGEQPLLNYESCNL
                     GSINLTKIVENGTINWEKLKRIVELTVRFLDNVIDVNQYPLKEIERMTLANRKIGLGI
                     MGWADLLAELEIPYGSKKSLTLAETLMGFINTTALEYSVYLGKIKGSFPNFKKSIYRD
                     TFEYLRNCTRTTIAPTGTISIIAGCSSSIEPFFAIAYGRKLANSSIFEINPVWEKKAA
                     TLKLLKPDITAEILKTGSLKNIRQIPSHIRNIFKTAFDIDPRLHLLMQATFQHHTDNA
                     VSKTINLPENATIQMVKNIFISAYKMKCKGITVFRYGSKSFQVLYLGEDFSQIDYLQI
                     GEHFSGCTKGYCNY"
BASE COUNT         1502 a          751 c         1025 g         1383 t
ORIGIN      
        1 gaagattcaa cacgcctgtg ttcactggca aacaggtaga ctgggtaata ttggtacaca
       61 catttttgat gcattgagga tgttaacagg acaacaacca gtggcagtca gtggaagttt
      121 agacccaacg ttataccctg attgcagggg acttcagtat aaggactatg gtggctgggg
      181 gatcattata ttttcagatg gaatgagggc atttattgat gcaccacaga caggcttatt
      241 tccacttgtc gtaaaaatta ctggagacag aggagaaatt attatccaga acaaggaggc
      301 agggatacat ttctggcagg gtggttcaca agaaattctg cctgagccag ggaataaaac
      361 agcccttgac aatgcgatgg aagacattgt cagttgtctt gtggaatcaa aagtgcctgc
      421 ctgtactggg aaagatggtc ttattgccct tgaagtaatt ataggatttc atgtttcaca
      481 ccgcttgggt ggaaaatgga ttgaccttcc aatcaaggga aatgacagaa attttgaagt
      541 aatgatggcg taattattca aagtcaaaaa aaatttgaaa tgaaattaaa gattttttct
      601 gtgtttgtgg tattttttct gtgcggttta ttatttggtt ctcctgagaa cactttttct
      661 attgtcaatc cttatgatgg tattgcttgg gaaaaagtgg ttcaatataa agccaattta
      721 catacccata ctactgtaag cgacgggaaa cttcatcccc agcacattgt tgatatttac
      781 gcttcgctcg gttataaaat tttatccatc acagaccaca gtgagatgaa caaaaatcct
      841 ggaaatatta cctatccctg ggaaaatttt cgtaatctgg tcacaagcgg tggaaaatca
      901 ggtttgtatg aaaacagaga cagcaaatta atgggtatgc ttgctgtgcc tggaagagaa
      961 atttcacagg aacatgatat tggcagttat ttcaatgtga gtatcgatat aaatagcgca
     1021 aaaaagatag aagatgttat agaaagtata gaaaaaaatc agggtcttgc tgtactatta
     1081 catcctggca gatattattt cactgatgat tggtatgtga atctgttaaa aaaatatcct
     1141 gtgattgtgg gaatggaggt atataaccag ggtgatagat accctggatg caaaataatc
     1201 tgggataatg tgttgacaaa attgatgcct gatagacctg tatggggttt ttcaaatgat
     1261 gatgctcata gatttgaaca tattggaaaa aactggaatg tttttttatt atcaaatcta
     1321 acaatagaag aattaaaaca tgcaatggaa aaagggcaat tttattttgc ctattgtcct
     1381 gatggaatta atagtactcc agcaataatc agaaagatag aagtggataa taagaatatg
     1441 ttgatacaca tagaagcaat aaattgcagt aagattgaat ggatttcaaa tggaaaaatc
     1501 gttggcactg gtccagaata tcagattaag acatcagatg gaaaagttgc taattatgtc
     1561 agggcaaaac tttatggaag tgataataaa tcagtgactg aactccagcc atttggcatt
     1621 caaaaaaagc gcctcataga gatgcaggcg aaacttgatt aaaagcaaaa aatggggata
     1681 atatatgtat cccagattta accctaaccg gaggaaaaat atgaaagttg tgatttcttc
     1741 attgagtgcg attgaaattc ttgattcaag gggtaatccc acaataaggg ttttctgctg
     1801 tttgagcaat ggaataaagg tttcaagttc agtgccatca ggcgcttcaa caggtgaaaa
     1861 cgaggcagtt gaattgaggg atggagacaa aaaacggtat ggtggaaaag gggtattgaa
     1921 ggcagttgaa aatgtcaata atatcattgc gccgaaaatt attggaattg aacctcacag
     1981 gcaggcagaa atagaccaga ttatgattga actggatggt acaccaaata aaggtcatct
     2041 tggcgcaaat gcaatacttg gtgtttcaat ggcagtggca aaggcgcagg caaaactttc
     2101 tggtcttcct ctgtatcaat atcttggtgg aactggcgca acgcggcttc ctgttccgat
     2161 gatgaatatt ctcaatggtg gaaaacacgc tgataatagt gttgattttc aggagtttat
     2221 ggtgatgcct gttggagcac cgaatttcaa agaagcattg agatatggtg ccgaaacatt
     2281 tcacgcgctt aagaaaattc tttcagaaaa aggttattca acaggggttg gagacgaagg
     2341 tggttttgct cccaaccttt caagcaatga tgaggcgtgc gaggtgattg ttaaggcaat
     2401 agaaaaagca ggatacaaac caggcattga tattgcaatt gcactggatc ctgctgcgag
     2461 ttctttttat gaagatggca tatacaatct gtcaaagtca aaacagggca aaaaaacttc
     2521 tgaggaaatg atagaactgt atggaaactg gataaaaaaa tatcccattg tttctattga
     2581 agacggactt gctgaaaatg actgggatgg gtttaagaag atgacagaaa aacttggaaa
     2641 taaaattcag attgttggtg atgacctgta tgtcaccaac acaaaatttg tggaaaaggg
     2701 aataaaggaa aaagccagca atgcagtgtt gataaagctc aatcagatag gtacagtgac
     2761 agaaacaatc cagacaatca atctttgcag aaaggcagga tggggttatg tcatatctca
     2821 tagaagcggt gagacagaag atacatttat ggcagatttt tctgttgcaa tggctggcgg
     2881 acagattaag acaggttctg catgcagaag cgaaagaatc gcaaaataca atagattgct
     2941 tgaaattgaa tgggaacttg gcaaatctgc tgtttttagt aatccctttc aacgggatta
     3001 gtttgggtaa aaattgattt tcaaactcag tgccaaccat aaaagggctc tggaaacagc
     3061 ccttacaatg cttgatgaac tcctggtttc tattgaaaaa tgggttgatg gaaaccagta
     3121 tcagtcagtg ctatatgaag agaaaaatga tttaagtgtt tcgcaaagaa aggcattgaa
     3181 aaaacaaatc cttgttttga aagcatctct tgaagccgcg aaaaaagacc ttggtttaac
     3241 aaaaaccacc caatctgcat tgaacgacat atggagcagg gttgcttcat tcagagaaaa
     3301 tgtcatggaa cttggagcaa gacacctgaa aagatatgga agtttgcacc atgaaacagc
     3361 agaatatatg gataaactat ctgaaaaact tctcttacaa cttgacggta ttcttgaaat
     3421 aatcaaagca ggcaggcact aataattgca gtatccttta gtacaaccag aaaagtgttc
     3481 accaatctga agataatcaa tctggctgaa atcttctcca aggtaaagca cctgaaatga
     3541 cttacttcca taacgaaaga cagtaattcc tttgcatttc attttgtatg cagaaatgaa
     3601 gatatttttt accatctgga ttgtggcatt ttcaggcaga ttgattgttt tagaaacagc
     3661 attatcagta tgatgctgga atgttgcctg catgagaaga tgaagacggg ggtcaatatc
     3721 aaacgctgtt ttgaatatat ttctgatatg gctgggtatc tgtctgatat ttttgagaga
     3781 acctgttttt aaaatttctg ctgtgatgtc tggtttcagt aatttgagtg ttgctgcttt
     3841 cttttcccag acaggattga tttcaaaaat acttgaatta gccagttttc ttccatatgc
     3901 aatcgcaaag aagggctcaa tactgctgga gcaaccagca ataatgctga tggtgcctgt
     3961 cggtgctatg gtggttcgtg tgcaatttct caagtattca aatgtatctc tgtagatact
     4021 ttttttgaaa tttggaaaac ttcctttaat ttttccgaga tacactgagt attctaatgc
     4081 agtggtgtta ataaaaccca taagtgtttc tgcaagcgtc aagctttttt tacttccata
     4141 aggaatttca agttcagcca gtaaatcagc ccatcccata atgccaaggc caatttttct
     4201 attggcaagc gtcatgcgtt ctatttcttt gagggggtac tgatttacgt caatcacgtt
     4261 atcaaggaac ctcacagtca gttccacaat tcgttttaat ttttcccagt tgatagttcc
     4321 attttcaaca atttttgtca gattgattga accaagattg caggattcat aattaagcag
     4381 gggctgttca ccgcagggat ttgtggcttc aatgcttcct atttctggtg tcgggttttt
     4441 tctgttaatc tcgtctataa aaatgattcc cgggtctcct gtttcccatg cacactggga
     4501 tataatgtca aacagttctc ttgccggtat gctttttaca gctgtttttg ttctcgggtt
     4561 taccagttca aatttttgat ttttaaaaac gctttccatg aatttatctg tgacagcaac
     4621 agaaagatta aagtttgtca gaatgttttt ccgttttgat g
//
LOCUS       DAORYU010000110         4638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_12565_length_4638_cov_6.787912, whole genome shotgun sequence.
ACCESSION   DAORYU010000110 DAORYU010000000
VERSION     DAORYU010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4638
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12565_length_4638_cov_6.787912"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="PLW07_04520"
     CDS             <1..575
                     /locus_tag="PLW07_04520"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HOL49822.1"
                     /translation="MNVDVKTPAGKDSKFGLGDIFIDPCIISWHSKNVHTSAGIDIIV
                     PTGAYDKNDIANIGRNYWTIEPVFAFTYLSDNGWDISGKLMYDINFENDATDYRSGQE
                     FHCDYTVAKRINNKWTLGLGGYYYAQTTDDKQNGVKVGDGNKGKVFAIGPQVKYDAGK
                     MSFILKYQKEMSVENKPEGNKIWLKVVAPL"
     gene            complement(580..1734)
                     /locus_tag="PLW07_04525"
     CDS             complement(580..1734)
                     /locus_tag="PLW07_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF763 domain-containing protein"
                     /protein_id="HOL49823.1"
                     /translation="MIKRGIATFTLDTGECPRWLFERMVKLGGQILEIIVQEYGPDEF
                     VARISDPVWFQSLGTVVAFDWNASGLTTVLTAALKETMKGREKQLGLFICGGKGKTSL
                     KTPDEIVQWGERIALPEKCVSDLVYSSRITAKVDSSLVQDGYQIYHHAFFFSRNGAWG
                     VIQQGMNQTNSTARRYHWFSSKINDFVCEPHSGIISQLIVPCLNLTAKESDKTRNIST
                     ELIQTGFSGLMKDIQILRKHSSRTTKMVAVKYGEQQMVFSEFKNTEFYYHPVVEEDFS
                     KSRYLEKILAKLCDYKPENYEKLIAVEGVGPKTVRALSLVAELIYNAPPSYIDPARYS
                     FAHGGKDGTPYPVDRGTYDKTIDFFRKVINKMKLSPSEKNTMLKKLQQSR"
     gene            complement(1700..2371)
                     /locus_tag="PLW07_04530"
     CDS             complement(1700..2371)
                     /locus_tag="PLW07_04530"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOL49824.1"
                     /translation="MAADFLDKAEKEIRTLYRDLLKKHGAPSESGQWKFWCKRPKTLQ
                     EKEIIIIESILTQRTKWNNVEKAVKNLMTHKLMSLQTILHADNENIESQIKSCGFSKQ
                     KTQRLKNIATFIIQSGGIENFEQLPTELLRDKLLSIHGIGKETADDVLLYAFERPVFV
                     IDEYTKRFVKTHNIATRFSYDFLQQLFEQSLKKDYKLYQDYHALIVIEMKEQNDKKRN
                     CNIYT"
     gene            complement(2349..3116)
                     /locus_tag="PLW07_04535"
     CDS             complement(2349..3116)
                     /locus_tag="PLW07_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010586441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOL49825.1"
                     /translation="MKNDTFNLAGKVALITGSGTGIGKAIAIKLAEHGAYIGVHYHSS
                     EKEALQTLETIKKMGVKTILLQADLTDEQQANNTVDKVVSKFGKLDILVNNAGTPVKR
                     SKIEQCPLELWNQVFSVNMTSAFMVTKRAIPHLRKTGKGSIVNIISLSVQTGGANGAG
                     PYAASKGALLVFTRTLARELAPEIRTNAVCPGVIETQHHEIFSTPERMEQYRAETPMK
                     RNGSPEEVANAVLYLVSDAASFTNGALLDVNGGRFLR"
     gene            complement(3106..4218)
                     /locus_tag="PLW07_04540"
     CDS             complement(3106..4218)
                     /locus_tag="PLW07_04540"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOL49826.1"
                     /translation="MTSRQRCKIAMKGGIPDCVPVIPQICHPHAIKVLGLDFRKTMIE
                     AIRNPYLVNELQIECARFYGVDGVRVWIHHEVIDNLYDNGENVWQIDPLTGKTIARLD
                     FKGGGKMIPLDEKVIIKNEQDIENIPVIDAETLLKDEKYEKTGKLIENAKKDLFVITS
                     PGPITVQFATTVRGKSQTFIDLIENPQLIKKILHRATKVAIEKSVAMLKLGVDAIMLG
                     DTFGGVIGPEFFKEFCVPYFKMFVDEIKKYDVCIYIHVCGNSTQLFELMAETGVDCIE
                     PLDPLGGVQVKDAKTRLLGKTAIMGGVNTLKLAHGTLDEVKQDIKRCLSEGAWQGGYI
                     LACGDMLPTEASPEKVFAMIESAHSYKYQQEANHEK"
     gene            4307..>4638
                     /locus_tag="PLW07_04545"
     CDS             4307..>4638
                     /locus_tag="PLW07_04545"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOL49827.1"
                     /translation="MADVFFASAKVRKLERNATLPAKFLRLLDKLEINNQVEEKTVVI
                     KMHMGTGYGFTTIHPLFVRMLVDYIRQHKGSPFITDIVMPDEKRGYTEQVMGCKFIPA
                     TGLRDTDY"
BASE COUNT         1272 a          925 c          875 g         1566 t
ORIGIN      
        1 taatgaatgt tgatgtgaaa actcctgctg ggaaagattc taaattcggt ttaggagata
       61 tatttattga cccgtgtatt atttcatggc attcgaaaaa cgttcataca tccgcaggaa
      121 ttgatattat tgttcccaca ggggcttatg ataaaaatga tattgcgaat atcggaagga
      181 attactggac aatagagcct gtttttgctt ttacatatct ttctgataat ggctgggata
      241 tttctggaaa acttatgtat gacattaatt ttgaaaacga tgcaaccgac tacaggtcag
      301 gccaggaatt tcactgtgat tatacagttg caaaaagaat caacaacaaa tggacactcg
      361 gacttggtgg atattattat gctcaaacca cagatgacaa acaaaatggt gtaaaagtgg
      421 gagatggaaa caaaggaaag gtatttgcca taggaccgca ggtgaaatac gatgctggaa
      481 aaatgagttt catcctgaaa taccagaaag aaatgtctgt tgaaaacaaa cctgaaggca
      541 ataaaatctg gttaaaggtt gtggctcctc tgtaaaatgt tagcgggatt gttgaagttt
      601 ttttagcatc gtgttttttt cagatgggga aagtttcatt ttattgatca cttttctgaa
      661 aaagtcaatg gttttatcgt aggtaccacg atcaacaggg tagggtgttc catcttttcc
      721 accatgagcg aatgaatacc ttgccgggtc aatgtacgaa ggcggagcat tgtaaatcaa
      781 ttcagcaaca agtgaaagtg ctcgtactgt tttgggtccg accccttcaa ctgcaatgag
      841 tttttcgtaa ttttctggtt tgtaatcaca tagttttgcg aggatttttt caagatacct
      901 gcttttcgaa aaatcttctt caaccacagg atgatagtaa aactctgtgt ttttgaattc
      961 tgaaaaaacc atttgttgtt ctccgtattt cacagcaacc atttttgttg ttctgctgct
     1021 atgttttctt aatatctgaa tgtctttcat taatcctgaa aaacccgtct ggataagttc
     1081 tgtactgata ttccttgttt tatcactttc ttttgcggtc aaattcagac atggtacaat
     1141 caactggctt ataattccag aatggggttc gcagacaaaa tcgttgattt tactggagaa
     1201 ccagtggtat cgtctcgcag ttgaattggt ttgattcatt ccctgttgaa ttactcccca
     1261 tgcaccgttt cttgagaaaa agaaagcatg atgataaatc tgataaccat cctgaacaag
     1321 actgctatca acctttgcgg taattctgga actatataca agatctgaaa cgcatttttc
     1381 aggcagtgct attctttctc cccactggac tatttcatcg ggtgttttga gcgaggtttt
     1441 gccctttccg ccgcatatga acaatcctaa ttgtttttcc cttcctttca tggtttcttt
     1501 caaggcggct gtcagaactg ttgttaatcc tgaggcgttc cagtcaaatg ccaccacagt
     1561 accgagtgat tgaaaccata cagggtcaga gattcttgca acaaattcat caggcccata
     1621 ttcctgtaca atgatttcca gaatctgacc cccgagtttt accattcttt caaacagcca
     1681 gcgcggacac tcacctgtgt caagtgtaaa tgttgcaatt cctcttttta tcattttgtt
     1741 ccttcatctc aataacaata agagcgtgat aatcctgata taatttataa tcctttttca
     1801 atgattgttc aaataactgc tgcagaaaat cgtatgaaaa tcttgttgca atgttgtgcg
     1861 ttttcacaaa cctttttgta tattcatcaa tgacaaatac tggtctttca aatgcataca
     1921 ataagacatc atctgcagtt tctttgccga ttccatggat ggaaagaagt ttatctctca
     1981 ataactcggt aggcaattgt tcaaaatttt caataccacc ggattggata atgaatgttg
     2041 cgatattttt caacctctgt gttttctgct tgctgaatcc gcaacttttt atctggcttt
     2101 caatgttttc attgtccgcg tgtaatattg tttgaagtga cataagttta tgagtcatca
     2161 aattctttac tgccttttca acattattcc attttgtcct ctgggtaaga atggattcaa
     2221 tgataatgat ttctttttcc tgtaacgttt tgggtctttt gcaccagaat ttccattgcc
     2281 ctgattctga tggggcacca tgttttttca aaaggtctct gtatagagtc ctgatttctt
     2341 tctctgcttt atctaagaaa tctgccgcca ttgacgtcta aaagtgctcc atttgtaaat
     2401 gatgcagcat cactgacaag gtaaaggaca gcatttgcca cttcttcggg gctaccattt
     2461 cttttcatgg gagtttctgc cctgtattgt tccattcttt caggggtgct gaaaatttca
     2521 tgatgctgtg tttcaatgac accagggcaa actgcatttg ttcttatttc aggagcaagt
     2581 tctcttgcca gtgttcttgt gaaaacaagt agtgcccctt ttgatgcagc atacggtcca
     2641 gcgccatttg caccgcctgt ctggactgaa agagaaatga tattaacgat agaacctttc
     2701 ccggtttttc taagatgggg aattgctctt tttgttacca taaacgcaga tgtcatattc
     2761 acactgaaga cctgattcca cagttccagc ggacattgtt caattttgct cctttttaca
     2821 ggagtacccg cgttgtttac aaggatatca agttttccaa atttactaac aaccttatca
     2881 accgtattgt ttgcctgttg ttcatctgta agatctgcct gcagcagaat tgtttttaca
     2941 cccattttct tgattgtttc gagtgtctgt aatgcttcct tttcactgga atgataatga
     3001 acacctatat atgctccatg ttctgcaagt ttgatggcta ttgctttacc gatgcctgtg
     3061 cctgagccag tgatcaatgc caccttaccc gccagattaa acgtgtcatt tttcatggtt
     3121 tgcctcctgc tgatatttgt aggagtgggc agattcgatc attgcaaaaa ctttttctgg
     3181 tgatgcttct gttggaagca tgtcaccaca agcaagaatg tatccaccct gccaggcgcc
     3241 ttcagagaga catcttttga tatcctgttt tacctcgtcc agtgttccat gagcaagttt
     3301 caaagtattt accccaccca taattgctgt tttacccagc aaccttgttt ttgcgtcttt
     3361 tacctgtact cctcccaatg ggtcaagggg ttcaatacaa tctacacctg tttcagccat
     3421 gagttcaaaa agttgagtac tatttccaca tacatgaata taaatgcaaa catcgtattt
     3481 ttttatttcg tccacgaaca ttttaaaata tggtacacaa aattccttga aaaattcagg
     3541 accgatcact ccgccaaatg tatctcccag catgattgca tcgactccaa gtttaagcat
     3601 cgcgaccgat ttttcaattg caacttttgt agcacgatga agaatttttt ttataagttg
     3661 tgggttttcg atgaggtcaa tgaatgtttg agatttacct cttacagttg ttgcaaattg
     3721 gacagttatt ggtccaggag atgttatcac aaacaggtct tttttagcat tttctattag
     3781 ttttcctgtt ttctcatatt tttcgtcttt caacaatgtt tcagcatcaa ttacaggaat
     3841 attttcaatg tcctgttcat tttttatgat gactttttca tccagtggta tcatcttgcc
     3901 accacctttg aaatcaagtc ttgcaatggt ttttccagtc aaagggtcaa tctgccagac
     3961 attttcgccg ttgtcataga gattgtcaat aacttcatgg tgtatccaga cccttacacc
     4021 atcaacaccg taaaatcttg cacattcaat ctgaagttca ttaacaagat acgggtttcg
     4081 aattgcttct atcattgtct tcctgaaatc aaggccaaga actttgatgg catgggggtg
     4141 acaaatctgg ggaattactg gtacacaatc cggtatacca cccttcattg caattttgca
     4201 tcgctgtctt gatgtcataa tgcctccttt gagggaaact aattgtcatt tataatatac
     4261 ctataattca aggtggtcaa aattttcgtc acaacaggaa agagcaatgg cagacgtttt
     4321 tttcgcctcg gcaaaagtac gaaaacttga gagaaacgct acacttcctg cgaaatttct
     4381 caggcttctc gataaacttg aaataaacaa tcaagtagag gagaaaactg ttgttattaa
     4441 aatgcatatg ggaactggtt acgggtttac cacaattcat cccctttttg taagaatgct
     4501 ggtggattat atcaggcagc ataaagggtc accttttatc acagatattg tcatgccaga
     4561 tgagaaaagg ggttatactg aacaggtaat gggatgcaaa tttattccag caaccgggct
     4621 tcgtgatact gattattt
//
LOCUS       DAORYU010000111         4559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_12828_length_4559_cov_4.517318, whole genome shotgun sequence.
ACCESSION   DAORYU010000111 DAORYU010000000
VERSION     DAORYU010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4559
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12828_length_4559_cov_4.517318"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3165
                     /locus_tag="PLW07_04550"
     CDS             <1..3165
                     /locus_tag="PLW07_04550"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF020121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOL49828.1"
                     /translation="IADDIKAADLKKATENKIALDRKFLYPFKGLPQFKNYKDFEQFI
                     NATLEVYSYGNGKILLLKGYMVPHFQNLTPANVRNPLETKYIEYDYLLAYPIHLMISS
                     VKQPEIEISGTDYVSADINSLSDITFILKSNEVKKVTCEFIVRNDDNEIISKDMKNLN
                     LAKGENEVKFAIRNKIPKGRYFADIWVKENNKIINFGSSFIELTSNEYIGDVVINKSY
                     KKEESISGEVSIFSKDGKDNLKLKISLIDNFGRLQQTEMMDLRGEKINEKKISFKIAP
                     PNIYFTVLQFLKFELFSDNNLVDVNSKFFSVSNLRQKENDFRAILWFPYYNAAYGEKW
                     YCKNIYDSGFDSRYYTGMSLFPFSENLWYVSNPTRFVDMKTDPWGTMDRKPEDHVRQP
                     CLNDPDYLSKVEKELKSKLENELHFSTYEFSCGDECHFASWRPPYDRYELCWCDRCVS
                     KFHAYLQNKYSTLQNLNRNWKTDYSSFDDIKPITYKEAIENPKLRPLWINYRIFMEDT
                     WAGIFEYAKQIITNMVPDAKVGYEGSQRGYTFVNSFTAEDYYKLAQAMNLNNPYSHAF
                     IRYAFKDFSKEGTLLGLGWQGSYGAGIKNRNIDPWRVLFFGANSLWIWHGPPREQSVV
                     SPDLSFYEYFRPSVNQIPELKNGIGKMFIHSKREDDGIGILYSNTSVHASALTPQLPP
                     ICENLNFFIRYFQGSGYQFRLVSYQQLEEGILKKEKFKVLILPFVQSISQKQAKEITS
                     FVNDGGVVIADIRPGICDENGVFYQKGGILDNIFGVTQNTENFEAKKFENLELKYGEL
                     TCNMPSIHVDASISLQNNGRTKENLENIPVFIVNNYGKGKGILLNVSLTNYPDENMAG
                     IRKILNNIIEQTAGIQKKINYEPDNLECGFTPYRYTLGSHTYLGILSPWREGEEKPCK
                     LTLSKKFHIYNIRKGEYLGFSDSLNINVQPETVKVIGFLPYRIMDVKINSKGKIKKGE
                     MLNYDIELVTEPQVPETHIFHISVISPEGKEIKHYAENLKGDKGKAKGSVHFALNEKQ
                     GKYVLRVKEIVSGITRDFPFEIAE"
     gene            3242..4387
                     /locus_tag="PLW07_04555"
     CDS             3242..4387
                     /locus_tag="PLW07_04555"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOL49829.1"
                     /translation="MNEKNLCSSCEVIRPSVKQDIIVYRDDEYCSNWPMSGGMWRFAD
                     REIIVGFTRLKVNYNQIGSTNHLFLDTWGELCLIRSKNDGETWNMDQVEVVVKKRDVA
                     IDFFQEKKQNLNLNPVDFTSPDTILLCNYVGEHMWAKSYSEVRFWSMILASPDRGRSW
                     PLGPIIKKPGHLFSSWGLPNYIVRKNGHVLIFNDCVMCGNEKEAQMHIYTDILENNGM
                     HWNFCGILPLERRDAKLVIHGSPVAISESSILLAARAQTACNIVYVMLYRSDDHGRNW
                     YTVGRVTDIGGTPHLLRMKDGRIVLTYERRFPPCGIRARVSEDNEGYQWGPEIVLRDD
                     GESDIGYSRSVQRHDGSILTVYYFVNASERTKDREPVRHIAGTIWQP"
BASE COUNT         1549 a          780 c          948 g         1282 t
ORIGIN      
        1 atcgcagacg atattaaagc agccgacttg aaaaaagcta cagaaaataa aattgctctt
       61 gacagaaaat ttttatatcc cttcaaagga cttccacagt ttaaaaatta caaagatttt
      121 gaacagttta tcaatgccac ccttgaggtt tattcttatg gaaacggaaa aattttgctt
      181 ctcaagggat acatggttcc acatttccag aacctgacac cagcaaatgt aagaaatcct
      241 cttgaaacaa agtacattga gtatgactat cttctggcat atcctatcca tctcatgatt
      301 tcctcagtca aacaacctga aattgaaatc tctggcacag attatgtgag tgcagacatc
      361 aattcactct cagatatcac atttattttg aaatcaaacg aagtgaaaaa agtaacctgt
      421 gaattcattg tgaggaatga tgataatgaa atcatttcaa aagacatgaa aaatttgaat
      481 ctggcaaagg gagaaaatga ggtgaagttt gctataagaa ataaaattcc gaaaggccgt
      541 tattttgctg atatatgggt taaggaaaac aataaaatta taaactttgg ctcttctttc
      601 attgaattga cctctaatga atacataggt gatgttgtaa taaataagag ttacaagaag
      661 gaagaaagta tttccggaga agtgagcata ttcagtaagg atggaaagga caacctgaaa
      721 ctgaagataa gtttgattga taattttgga agactgcaac agacagaaat gatggatttg
      781 aggggagaaa aaatcaatga aaagaagata agttttaaga tagccccacc gaacatatat
      841 tttacagtac tacaattttt gaaatttgaa ctgttcagtg ataacaatct ggttgatgta
      901 aattcaaagt ttttctctgt ttcaaacctg cggcaaaaag aaaatgattt cagggcaatt
      961 ttatggtttc cgtactacaa tgctgcctat ggagaaaaat ggtactgcaa aaatatctat
     1021 gattctggat ttgactcaag atattacaca ggcatgtctc tttttccatt ttcagaaaat
     1081 ctgtggtacg tttcaaatcc aacaagattt gttgatatga aaacagatcc gtggggaaca
     1141 atggatagaa aaccagagga ccatgtgaga caaccgtgct tgaatgaccc tgattacctg
     1201 tcaaaagtgg aaaaagaatt gaaaagcaaa ctggaaaatg aactgcattt ttcaacatat
     1261 gaattttcct gcggagacga atgccatttt gcaagctgga gaccacctta tgacaggtat
     1321 gaattatgct ggtgcgatag atgcgtttca aaatttcacg catatctgca gaataaatac
     1381 agcactttac agaatttgaa caggaactgg aaaacagatt atagttcgtt tgatgatatt
     1441 aaacccatca catataagga agccattgaa aatccaaagt taagaccatt gtggataaat
     1501 tatagaattt ttatggaaga tacctgggca ggtatttttg aatacgcaaa acagattatt
     1561 acaaatatgg ttcctgatgc aaaggttgga tacgaaggaa gccagagagg ttataccttt
     1621 gtcaatagtt ttactgctga ggattattac aaacttgctc aggctatgaa tctgaacaat
     1681 ccatatagcc acgctttcat cagatacgca tttaaggatt tttcaaaaga agggacactt
     1741 cttggacttg gatggcaggg ttcttatgga gccggaataa aaaacagaaa cattgaccca
     1801 tggagagtac ttttctttgg tgctaacagt ttatggatat ggcatggtcc tccaagagaa
     1861 caatcagttg tttctcctga cctgtcattt tatgagtatt ttagaccctc tgtaaatcaa
     1921 atacctgaat taaaaaatgg catcggcaag atgtttattc attcaaaaag agaggatgat
     1981 ggaataggaa tactttattc aaatacaagt gttcatgctt ctgctttaac cccgcaactt
     2041 ccgcccatat gcgagaacct gaacttcttt ataaggtatt ttcaaggaag tggttaccaa
     2101 tttcgtctgg tatcctacca gcaacttgaa gaaggtatcc tgaaaaaaga gaaatttaag
     2161 gttttaatcc ttccatttgt ccaatccatc tcccaaaaac aggcaaaaga aataacttct
     2221 tttgttaatg atggtggagt ggtcattgct gatataagac ctggtatatg tgatgaaaac
     2281 ggtgttttct atcaaaaagg cgggatcctt gacaatattt ttggagtaac gcagaacaca
     2341 gaaaattttg aagcaaaaaa gtttgagaat ttagaactta aatatggtga attaacctgc
     2401 aatatgccct caatacatgt ggatgcctca atcagtttgc agaataatgg tagaacaaaa
     2461 gaaaaccttg aaaatatccc tgtttttatc gtcaacaatt atggcaaagg aaagggaatt
     2521 ctgttgaatg taagtttgac aaattatcct gatgaaaata tggcagggat aaggaaaatc
     2581 ctcaacaaca tcatagaaca gaccgcaggg attcagaaaa aaatcaatta tgaacctgat
     2641 aatcttgaat gtggctttac tccttacaga tacacacttg gttctcatac atatctcggg
     2701 attctttctc catggagaga aggagaagaa aaaccctgta aactaaccct gtcgaaaaaa
     2761 tttcacattt ataacataag aaagggagaa tatctgggtt tcagtgattc attaaacata
     2821 aacgttcaac ctgaaacagt aaaggtgatt ggctttttgc cttacagaat catggatgta
     2881 aaaattaact caaaagggaa aataaaaaag ggtgaaatgt tgaattatga cattgaactg
     2941 gtgacagaac cacaggtacc tgaaacgcat attttccata tcagtgttat ttcccctgaa
     3001 ggaaaagaaa taaaacatta tgcggaaaac ctgaaaggag acaaaggaaa ggcaaaaggc
     3061 agtgtacatt ttgccttgaa tgaaaaacag ggaaaatatg tattaagagt caaggaaatc
     3121 gtttctggca ttaccaggga tttcccattt gaaatagcag aataaaaaca aaaaattggt
     3181 ctaatgatgt tttgattgtg ttgtgctata ataaacatcg atttctgcca cgcaacagga
     3241 tatgaatgaa aaaaaccttt gttccagctg tgaagtaatt agaccgtcag taaaacagga
     3301 tattattgtt tacagggatg acgagtactg ctcaaactgg cccatgagcg gcggtatgtg
     3361 gcgttttgct gacagggaaa tcattgtcgg gttcacacgt ctcaaagtaa attataacca
     3421 gattggttcc acaaaccacc tgtttcttga tacctggggt gaattatgtc tcatacgttc
     3481 aaaaaatgat ggcgaaacat ggaatatgga ccaggttgaa gttgtggtaa agaaacgcga
     3541 tgtggcaatt gacttctttc aggaaaagaa gcagaacctg aacctgaatc ctgttgattt
     3601 cacatcccct gatactattt tgctgtgcaa ttatgttggc gaacatatgt gggcgaaaag
     3661 ttacagtgaa gtgcgattct ggtctatgat tctggcatct cctgacaggg gcagatcctg
     3721 gccgctcggt cctatcatta aaaaaccagg acaccttttc agtagctggg gactcccgaa
     3781 ttatatcgtc cgtaaaaatg gacatgtttt aattttcaat gactgtgtga tgtgcggaaa
     3841 tgaaaaggaa gcccagatgc acatctacac tgacattctt gaaaacaatg gtatgcactg
     3901 gaatttctgt ggtatacttc ccctggaacg ccgcgatgcg aaattagtca ttcatggatc
     3961 ccctgtcgcc atcagtgagt cctcgattct tcttgcagca cgtgcgcaga cagcgtgtaa
     4021 cattgtttat gttatgctct atcgcagtga tgatcacggt agaaactggt acactgttgg
     4081 ccgtgtcaca gacataggtg gcacgcctca tctcctaaga atgaaagatg gcaggattgt
     4141 cctgacatat gaacgccgtt ttcctccatg cgggattcgt gcacgggtat ctgaagacaa
     4201 tgaaggatat cagtggggac ctgaaattgt attgcgagat gatggcgaaa gcgatattgg
     4261 atacagccgg agtgttcagc ggcacgatgg ttcaatactc acggtttatt actttgtcaa
     4321 tgcatctgaa cgcacaaagg accgtgaacc agttcgtcat atcgcaggaa ccatatggca
     4381 accataaatt tttaatcacg agagatgaac ggaaaacaga aatttttgaa aattgctcgt
     4441 tttgaattat ctggcgcgat tttcctacct tcctgctgga actggttctg ggaacgaacc
     4501 atagaaaggt ggaagaaaga aggtttgcct ggagatgttc atactgaaga atattttgg
//
LOCUS       DAORYU010000112         4469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_13123_length_4469_cov_4.504304, whole genome shotgun sequence.
ACCESSION   DAORYU010000112 DAORYU010000000
VERSION     DAORYU010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4469
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13123_length_4469_cov_4.504304"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..1169)
                     /locus_tag="PLW07_04560"
     CDS             complement(156..1169)
                     /locus_tag="PLW07_04560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOL49830.1"
                     /translation="MRKENYRTGESGRILIIDGTDIENCLLLTPVIRQIKEQDSCLKI
                     DVVATTSSEKFLKEYKWFDRIIVHKKTGFFKTYKSLYSERYRIIISFHPSIIPYILRA
                     KTKLNSLKTRIFTDRFFTHESINFLRLIEPLFGKPVRVELFFPISETDREKVKEFLKS
                     KNISNSSTLIALHTGDKDAPDKWNISNYIEVCDTLIEEYNAKILIFGNAGDSVIGKII
                     SSTKYPHDIIDLSQIENFREMAAFLSRANLAITRNGFFLYLACAVKTPVVTIFGAGNP
                     YRFGPVGTRYINIHSQLDCFPCSKKRKCNKNYQCIKSITSDAVIEAARLILDENKQLY
                     LFE"
     gene            complement(1124..2197)
                     /locus_tag="PLW07_04565"
     CDS             complement(1124..2197)
                     /locus_tag="PLW07_04565"
                     /inference="COORDINATES: protein motif:HMM:NF018017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ELM1/GtrOC1 family putative glycosyltransferase"
                     /protein_id="HOL49831.1"
                     /translation="MKILILDDGIPGNTNQSVGVAEATGYEFHILPVNLKGLSYKLPG
                     RKGSTKIASKLIGSLLKVNAYRWADFVLKISSKTDIPAEKFDIVISAGSFLAPINLVI
                     SRKVGAKAISIMTPEMTPLKQFDLLIVPVHDTLRYPFLAKLQNLFVTIGAPNRINADL
                     LKSAMSKMLNKIKIPETPKIGVIIGGNDQNYFIDIEWARKLLSLLKMLAERKFVFFLT
                     TSRRTPANVVHFLKEKTNIDNFVYREFPGISPDSHYFGILGICDILFVTEDSITMMSE
                     VCSTGKPVIILGTGRKKRNIVFDTTIETLVKNKYCLHVPVDKFEQIPDILLKISNENP
                     FPILEDSKQCAKRIIELVNQEGS"
     gene            complement(2204..2965)
                     /gene="hisF"
                     /locus_tag="PLW07_04570"
     CDS             complement(2204..2965)
                     /gene="hisF"
                     /locus_tag="PLW07_04570"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOL49832.1"
                     /translation="MLAKRIIPCLDVKDGNVVKGIHFENLKHAGEVVDLARYYSNEGA
                     DELVFLDISATSEGRKTLKNLVKKVASNISIPFTVGGGIKTIDDILVLLENGADKVSI
                     NTNAVKNPNIICEAAKKFGSQCIVIAIDAKRIAANRWNVYIESGKIATEINVQEWAKK
                     VEDLGAGEILLTSIDTDGTLTGYDFQLTHTITKTVKIPVIASGGAGSLEHLYRVLAES
                     NASAVLLASLLHFKTFTIKQIKKFLAEKNIPVRLN"
     gene            complement(2967..3572)
                     /gene="hisH"
                     /locus_tag="PLW07_04575"
     CDS             complement(2967..3572)
                     /gene="hisH"
                     /locus_tag="PLW07_04575"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOL49833.1"
                     /translation="MTGIINYGAGNIFSVIVSIKNLHNKVTVVSKPETLKNVDRIILP
                     GVGAFDHAMKYLEQTGLSQAIREETRKGKFLLGICLGLQMFFENSEEGKSKGFGFIRG
                     KVKKLSGKKNLPVPHMGWNSIDITRNSKFLKDIPDNSFFYFAHSFYCFPKEKNTALAT
                     TSYGTEFASVVQKDNIIGVQFHPEKSGKYGLLLIKNFLEMK"
     gene            complement(3627..>4469)
                     /locus_tag="PLW07_04580"
     CDS             complement(3627..>4469)
                     /locus_tag="PLW07_04580"
                     /inference="COORDINATES: protein motif:HMM:NF013151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExsB family transcriptional regulator"
                     /protein_id="HOL49834.1"
                     /translation="MVCFEIARNATIKQEGQTIPFIIDTGLMRQNEVKQTEEYFRKKY
                     NIELQRWDKQNEFISALKGIVSPEEKRKTFRDIFYRIISQAMRSYAADILVQGTILSD
                     IIETQKGIKTHFNVLHEAGINPANYGLSLFEPLKELTKSRVRMVARSFAIPPKFLRIQ
                     PFPGPGFALRITGEVTFERVAKIRKATEIVESELMSPKIFQGFPVLLTDKATGIQNES
                     AFVGDIIAIRIVESKDSLSARSYRIPWIKMDKIAKRILTEIPGIARVLYDVTSKPPGT
                     IEFV"
BASE COUNT         1275 a          842 c          776 g         1576 t
ORIGIN      
        1 tgattttttc tttttccatt aaagggatat aactacctgg tcctgaaata atggtgactt
       61 caatacccat ccttttcata ccgcatgcaa ggtcaaatgt gccttcttct acaccaccaa
      121 gatgcatttc aggaagcatc agaaagatct tcatgctatt caaacaggta tagttgcttg
      181 ttttcatcaa gaataagacg ggcggcttca ataacagcat cagaagttat acttttgatg
      241 cattgatagt ttttattaca tttacgcttt ttactgcaag ggaaacaatc cagttgactg
      301 tgtatattta tatatcgtgt tcctacagga ccaaacctat atgggtttcc tgccccaaaa
      361 atggtaacaa caggtgtttt gacagcacag gcaaggtaca ggaaaaagcc gtttcgcgtg
      421 attgccagat tggccctgga caaaaacgca gccatctcac gaaaattttc tatttgtgac
      481 aggtcaataa tatcatgcgg atactttgtt gaactaatga ttttaccaat gacggaatcg
      541 ccagcatttc cgaaaataag aattttcgca ttatactctt caataagagt gtcacaaact
      601 tcgatatagt tggaaatatt ccacttatcc ggagcatctt tatcacctgt atgaagggca
      661 atcaatgttg atgaattgct tatgtttttt gactttaaaa attctttcac tttttctcta
      721 tcagtttcgg aaataggaaa aaatagttca accctgacgg gtttcccgaa cagtggttca
      781 atcaatcgta aaaagttaat actttcgtgc gtaaaaaacc tgtcagtaaa aatccttgtt
      841 tttaaagaat ttaacttcgt ttttgcacgc aaaatataag gaattattga tggatgaaac
      901 gatatgatga ttctatatct ttctgaatac agggacttat aggttttaaa aaaccctgtt
      961 ttcttatgca caatgattct gtcaaaccat ttatactctt tcaggaattt ttctgagctg
     1021 gttgtcgcaa caacatcaat tttgaggcaa ctatcctgtt cttttatttg ccttatgaca
     1081 ggtgtcaata atagacagtt ttcaatgtca gttccgtcaa taatcaggat ccttcctgat
     1141 tcaccagttc tataattctc tttgcgcatt gttttgaatc ttccagaatg ggaaaagggt
     1201 tctcattaga aatttttaat aaaatgtctg gaatttgttc aaatttatca accggtacgt
     1261 gcaaacaata tttgttttta acaagagttt ctatggttgt atcaaaaaca atgtttcttt
     1321 tctttctgcc agtacccaga atgataacag gttttccggt cgaacacacc tcagacatca
     1381 ttgtaatact atcttcggta acaaaaagaa tatcacagat tcccagaatt ccaaaatagt
     1441 gtgaatcagg acttatgcct ggaaattccc gataaacaaa attgtcaata ttagtttttt
     1501 ctttcaaaaa atgaacgacg tttgcgggtg ttcttcttga agttgtgaga aaaaaaacaa
     1561 actttctttc tgccagcatt ttcaataagg atagaagttt gcgtgcccat tcgatatcta
     1621 taaaataatt ctgatcattg ccccctatga taacgcctat tttaggtgtt tcaggtattt
     1681 ttattttgtt aagcattttt gacattgcgg atttcagtaa atcggcattg attctatttg
     1741 gtgctccaat tgtcacaaat aagttttgca gtttagcaag aaaaggataa cgaagggtat
     1801 catgtaccgg cacaatcaag aggtcgaatt gtttcagtgg ggtcatttca ggtgtcatga
     1861 tgctgattgc ctttgcacca acttttctgg aaataacaag gttgatgggc gcaaggaaag
     1921 aacctgcaga aattacaata tcaaattttt ctgccggtat atcagttttt gaagatattt
     1981 tgagaacaaa atcagcccat ctgtaggcat taacttttaa taaagagccg atgagttttg
     2041 atgcaatttt tgttgaacct tttcttcctg gcagtttata tgataatccc tttaagttta
     2101 ctggcaaaat atggaattcg taacctgttg cttcagcgac tccaacagat tgattggtat
     2161 tgccaggtat accatcatcc agaataagaa ttttcatggt attttagttc agtcgcacag
     2221 gaatattttt ttccgccagg aattttttta tttgttttat tgtaaatgtt ttgaagtgaa
     2281 gcaaagatgc aagcaatacc gctgaagcat tactctcagc aagtaccctg taaaggtgtt
     2341 cgagcgaacc cgctcctcct gaagcgatta ccgggatttt tactgttttt gtaattgtat
     2401 gggtcaattg aaaatcatat ccggtcagtg taccatcagt gtcaatactt gtaagaagaa
     2461 tttctcctgc accgaggtcc tcaacttttt ttgcccattc ttgcacattg atttctgtgg
     2521 caatttttcc ggattcgata tagacattcc atcgattagc tgctatcctt ttcgcatcaa
     2581 ttgcgatgac aatacattga cttccaaatt tttttgcggc ttcacaaatg atatttggat
     2641 tttttaccgc attggtgtta atggaaacct tatcggctcc attttctaat agaacgagaa
     2701 tatcatcgat tgttttgata ccgcctccca ctgtaaatgg tatagaaata tttgatgcaa
     2761 cttttttaac cagatttttt aatgttttac gtccttcaga tgttgcactg atgtcaagaa
     2821 atacaagttc atctgctcct tcattgctat aataccttgc aaggtctacc acttctcctg
     2881 catgtttaag attttcaaaa tggattcctt ttaccacgtt tccatctttg acatccaggc
     2941 agggaattat tctttttgca agcatattat ttcatttcca agaaattttt tattaaaagc
     3001 aatccgtatt ttccactttt ttcggggtga aactgtactc caataatatt gtctttttgt
     3061 acgacagatg caaattctgt tccataagat gttgtcgcca gtgcggtatt tttttctttc
     3121 gggaagcagt aaaacgaatg agcaaaataa aaaaaagaat tgtctggtat atctttgaga
     3181 aattttgagt tgcgtgtgat atcaatagaa ttccatccca tatgcggaac aggtaaattt
     3241 tttttacctg atagtttttt gactttacct ctaataaacc caaatccttt tgattttccc
     3301 tcttcactgt tttcaaaaaa catctgtaat ccaagacata ttccaagaag aaatttgcct
     3361 ttccttgtct cttctcttat tgcttgtgat aaccccgttt gttctaaata tttcattgca
     3421 tggtcaaagg caccgactcc tggtagtata attctatcaa catttttcaa tgtttctggc
     3481 tttgacacga ccgtcacctt attgtgcaaa tttttgatgc tgactataac actgaaaatg
     3541 ttacccgcac cgtagtttat tataccagtc atattctcat attcgaatta gaatttataa
     3601 gttacaggat gaagcatata cagcagttac acgaattcta tagtaccagg tggttttgat
     3661 gttacatcgt aaagaaccct tgcgataccc ggaatttccg tgaggattct ttttgcaatt
     3721 ttatccattt ttatccaggg gattctataa gacctggcgg acaaagaatc ttttgattcc
     3781 acaattctga ttgctatgat atcgcccacg aatgccgatt cattttgaat accggtcgct
     3841 ttatcagtta gtaacacagg gaaaccctgg aaaatttttg ggctcatcag ttcggattcc
     3901 acaatttcag tggcttttct gatttttgca acccgttcaa aggtgacctc ccctgtgatt
     3961 ctcaatgcaa aaccaggacc gggaaatggt tgaattctca agaattttgg aggtatggca
     4021 aaacttctgg caaccatcct tactcgagat tttgttagtt ctttcagtgg ttcgaataga
     4081 cttaacccgt agttagcggg attgattcct gcttcgtgca atacattgaa atgtgttttt
     4141 attcctttct gagtttcaat gatatccgag agtattgtgc cctgtaccag tatatccgct
     4201 gcataacttc gcattgcctg acttataatt ctataaaaaa tgtctctgaa tgtttttctt
     4261 ttttcttcag gagagactat tccttttaat gctgaaatga actcattttg tttatcccat
     4321 cgttgaagtt caatattgta tttttttctg aaatattctt cggtttgttt gacttcattc
     4381 tgtctcataa gacctgtatc gataataaat ggtatcgttt gtccttcttg ctttattgtg
     4441 gcgttcctgg caatttcaaa acagaccat
//
LOCUS       DAORYU010000113         4394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_13389_length_4394_cov_6.787739, whole genome shotgun sequence.
ACCESSION   DAORYU010000113 DAORYU010000000
VERSION     DAORYU010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13389_length_4394_cov_6.787739"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..1110)
                     /locus_tag="PLW07_04585"
     CDS             complement(94..1110)
                     /locus_tag="PLW07_04585"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HOL49835.1"
                     /translation="MHICIDLHYDVPLDFFYRHQNGEKNIFSKYHLEKIQQGGVNCFI
                     APIYVEPWFKPDKALRRGMETLEIILEEIENTRQLVKIEKFDDFKQLQDAEKIGIIIG
                     VEGGEIIEDSFYLLDVYYRLGVRCFGFVHGEPNLVADAGSFQNSKGGIFEFGRKLIKQ
                     LENRGWIIDGAHLPSYGLDQLIEIATKPFLISHTLLENPGRNVGLSRSQVEKLAEKGC
                     LIGLAVLNIHQENEEMGAVPDIHEYVKCLNYIKNITGSVDCIGLGFDFYDFLDAGFIK
                     RRYPGLVFDRVEGLEGIQDTKNLFPLLENSGYSQTDIEQIKGKNFINLVRKLWTTGVQ
                     NEKN"
     gene            complement(1130..2788)
                     /locus_tag="PLW07_04590"
     CDS             complement(1130..2788)
                     /locus_tag="PLW07_04590"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOL49836.1"
                     /translation="MKPINPVKKDDGLYFADMSKCYPSSAISEKRGRKNWRKFHYETE
                     KMAGVLLVSCYGVNPPVLSLPVKGRGRYKIFVGLYGEFGFYAGIRIKLKKDYCFHHLV
                     SWNSLSKGAVINEVFWKEAEMIDEVIEIAAYSKKDEDRITSGIAYIRLVPAEKKIASD
                     RKPDIYLAAFNDAYSFIYYHRPVTKKEIQEQIEVFRDTPFKKIYWGMGAGDVATYPTN
                     VGSRANIDLDVYPRIGDRNYAESCRILRNKNLDILKIAIEHAKKIGLEFHVYHRLGFV
                     AGQPPWEEMMYSKFFYENQRFLCYDRDGTKISRLSYAYLEVQQHCINLYKEVASYGID
                     GINFAFHRGVPLVLFEKPVVEGFKSRYGEDPRKVPDTDQRLFEFRSSIITGYMERVRK
                     EFPGLELTATVLINEANNRFYNLDIKNWIDSRTIDVLIPYGQDREIAHKGVIDMEYFS
                     KLTKGTSCQLCPELRQVRSWMTSEQHYIDIAEYFYKYNIDGICFWDIETRFIFPREWR
                     IINELCRKKGLKNRVNRVKNDFRDIPLKTLNGYAANRYPFWWSY"
     gene            complement(2785..>4394)
                     /locus_tag="PLW07_04595"
     CDS             complement(2785..>4394)
                     /locus_tag="PLW07_04595"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOL49837.1"
                     /translation="AWLVTSEDPQTKKPQDNACVRIIAPEPDTVVTIKGIEDKRAFGK
                     LAEKEETRIELKDKKFIEINYGNAWGFREITSTKPIFVYVTPSSLNNFTMLHPTYESG
                     KEKYTGKEFYGGINPYPNNNFYPCIVITCIDDSADVYLEEMDDDGVVVKKTQASKTLK
                     QGEEYIFYLASGVPWYHIVSTGDICAYTSRAMLNGNAYTRDTLFLPDVNGVFCGTDFI
                     ANIGMETLVYSSKPDCELTVYSFDGQFAPFKMQLEKGYSLLGCPRVYGEKQYLISFSS
                     PGLVQFNSAYSMSYAMPVDGPVASVYLGSEKVKRVQAPAQNRPVVVKKVDNEYTGLSV
                     EQKISRFKNEYLMKKKPVKIVGFGDSVTQSARVSGAETFYQIFTKQLKEKYGYDSITA
                     VNRGIGGNNVFHALSRIREDVVAEHPQIVFVMFGVNDGYRGMNMDVYEKNMRKIIEIL
                     ENETDALIILLGPTPLTTSKDALRDYDKTVEKLGKEKKIPYLSMHEVLMKDPEWDKKL
                     YTDFCHPGPQGHKMMAEGIWKFFEGGK"
BASE COUNT         1235 a         1013 c          691 g         1455 t
ORIGIN      
        1 gtggcaacat atgtattgat tgttttgtcc tgcatatttt gttgctggca taacaattga
       61 gcaacagaaa caaggaaaca tacgccaatc aagctaattt ttttcatttt gtactcctgt
      121 tgtccacaat tttctgacaa gattgatgaa atttttcccc tttatctgtt caatgtctgt
      181 ctgtgaataa cccgagtttt ccagcaaggg aaacaggttc tttgtatcct gaattccttc
      241 aagaccttca actctgtcaa aaacgagacc agggtatctc ctctttataa aacccgcatc
      301 cagaaaatca tagaagtcaa aaccaagtcc aatacagtca actgaaccag taatattttt
      361 tatgtaattc agacatttca catattcatg aatatcaggc acagcaccca tttcctcatt
      421 ttcctgatga atattaagca cagccagacc aatgagacat cctttttcag caagcttttc
      481 tacctgactt ctggacaggc ccacatttct cccaggattt tcaagcaggg tatgagatat
      541 caaaaatggt tttgtcgcaa tttcaataag ttggtcaagt ccatatgatg gcagatgcgc
      601 tccgtctata atccatcccc tattttccag ttgtttaatt aattttctcc cgaactcaaa
      661 gataccacct tttgaattct gaaacgaacc tgcatccgca acaagatttg gttctccatg
      721 tacaaaacca aagcaacgca cgccaagcct gtagtaaacg tcaagaagat aaaaactatc
      781 ctcaataatt tctccacctt ccactccaat gataatacca attttttctg cgtcctgtaa
      841 ctgcttaaaa tcatcaaact tctcgatttt caccagttgc cgtgtatttt caatttcttc
      901 aagaattatt tcaagggttt ccattcccct tctcagggct ttatccggtt tgaaccaggg
      961 ctccacataa ataggtgcaa taaaacagtt caccccaccc tgttgaattt tttccaagtg
     1021 atattttgaa aaaatatttt tttctccgtt ctggtgtctg taaaaaaaat caagcgggac
     1081 atcgtaatga aggtctatgc aaatatgcat tactcctcac tttaaaccac taatatgacc
     1141 accagaacgg atatctattt gctgcgtatc cattcagggt ttttaagggg atatccctga
     1201 aatcattttt caccctgttg accctgtttt tcaatccctt tttccggcac aactcattaa
     1261 taattctcca ttccctcgga aatataaatc ttgtttcgat gtcccagaaa cagataccat
     1321 caatattgta tttataaaaa tactctgcaa tatcaatgta atgttgttct gatgtcatcc
     1381 aggaccttac ttgccgcaat tcaggacaca actggcagga agtccccttt gtaagttttg
     1441 agaaatactc catgtcaatc acgcctttat gggcaatctc cctgtcctgg ccataaggaa
     1501 taagaacatc tatagtcctg ctgtcaatcc agttctttat gtcaagatta taaaatctgt
     1561 tattagcttc gtttatcaaa acagtcgcag taagttcgag tccgggaaat tcctttctca
     1621 ccctttccat atagcctgta ataatactgc ttctgaactc aaaaagacgc tggtctgtat
     1681 caggtacttt tctcgggtct tctccatatc tgctcttaaa cccttccaca accggtttct
     1741 caaaaagaac aagtggtaca cctctgtgaa aggcaaagtt tatcccatca attccgtaag
     1801 aagcgacttc cttataaagg tttatacagt gttgctgcac ttcaagatac gcataactta
     1861 accgtgaaat ttttgttcca tctctgtcat agcataaaaa tctctgattc tcataaaaaa
     1921 actttgaata catcatttct tcccagggtg gctgtcctgc tacaaatccc agcctgtgat
     1981 agacatgaaa ttcaagccct atcttctttg catgttctat ggctatcttc aaaatgtcaa
     2041 ggtttttgtt tctcaaaatc ctacaacttt ctgcataatt tctgtcccca atccttggat
     2101 atacatcaag atcaatattc gctcttgaac caacattggt tggatatgtg gcaacatctc
     2161 cagcacccat tccccagtag atttttttaa aaggtgtatc gcgaaaaacc tctatctgtt
     2221 cctggatttc cttttttgtt acgggtctgt gataatagat gaaagaataa gcatcattga
     2281 atgccgcaag atatatgtct ggttttctgt ccgatgcaat ctttttttca gcaggcacaa
     2341 gcctgatgta tgcaatacct gaggtaattc tatcttcatc ttttttgctg taagccgcaa
     2401 tttcaatcac ttcgtcaatc atttctgctt ctttccagaa cacctcatta ataacagctc
     2461 cctttgaaag ggaattccac gaaacaagat gatgaaagca ataatctttc ttcagtttaa
     2521 tccgtatacc agcataaaat ccgaattctc cgtaaagtcc cacgaaaatt ttgtatctac
     2581 ctctgccttt tacaggtaaa ctcaatactg gcgggttcac accataacag gaaacaagta
     2641 acacacctgc cattttttct gtttcataat gaaattttct ccagtttttt ctgccccttt
     2701 tttcagaaat cgctgaagaa ggatagcatt tgctcatgtc agcaaaataa agaccatcgt
     2761 ctttctttac tggatttatc ggtttcattt tcctccttcg aaaaatttcc agattccttc
     2821 tgccatcatt ttatgtccct gtggtcctgg atggcagaaa tcagtgtaaa gtttcttatc
     2881 ccattctggg tctttcatca aaacttcgtg catactcaga tatggaattt ttttctcttt
     2941 gcccaatttt tccacagttt tatcatagtc ccttaaggca tctttgctgg tggtcaatgg
     3001 agttggaccc agaagtataa tcagggcatc tgtctcattt tccagaatct caataatttt
     3061 cctcatattc ttttcataaa catccatgtt cattcccctg taaccgtcat ttactccaaa
     3121 catgacaaag acaatctggg ggtgttcagc aaccacatct tccctgattc tgcttaacgc
     3181 atggaaaaca ttgttaccgc cgatacctct gttcactgca gtaatactgt catatccgta
     3241 tttttctttc aattgttttg taaatatctg ataaaatgtt tcagcgccac ttacccttgc
     3301 gctctgtgtc acagaatctc caaatccaac aatttttact ggttttttct tcatcaggta
     3361 ttcattttta aaccttgaaa ttttttgctc aactgaaaga cctgtatatt cgttatccac
     3421 ctttttcaca accacaggtc tattctgtgc aggcgcctga acccttttta ctttttcaga
     3481 accaagatac actgatgcca caggaccatc aacaggcatc gcataactca tggagtatgc
     3541 agaattgaac tggacaagac ccggacttga aaatgaaata agatactgtt tttcgccata
     3601 aaccctcgga catccaagca gtgaatatcc cttttcaagt tgcattttga aaggcgcgaa
     3661 ttgtccatca aaactgtata cagttaactc acaatctggt tttgaagaat atacaagagt
     3721 ttccattcca atgtttgcaa tgaagtcagt tccgcagaat acaccattga catcaggcaa
     3781 aaacagagta tcccttgtgt acgcattccc attcagcatt gctcgtgaag tgtaggcgca
     3841 tatatcgcct gttgaaacaa tgtggtacca tggaacccct gacgcaagat agaaaatgta
     3901 ctcttctccc tgcttcaggg tttttgatgc ctgtgttttt ttcacgacca caccatcgtc
     3961 gtccatttct tccagataaa catctgcgct gtcgtcaatg catgttatga cgatgcaggg
     4021 ataaaaatta ttgtttggat atggatttat tcccccataa aattcttttc cagtgtattt
     4081 ttcctttccg gattcatagg ttggatgcaa catcgtaaaa ttgttaagag aagatggtgt
     4141 aacataaaca aaaattggtt ttgtggatgt tatttctcta aatccccatg cattcccata
     4201 attgatttca ataaatttct tatccttcaa ctcaatcctt gtttcttctt tctcagccag
     4261 ttttccaaac gctcttttgt cttctattcc cttaattgtt acgacagtgt ctggttcagg
     4321 agcaataatc ctcacacatg cattatcctg gggctttttt gtttgtgggt cctcagaagt
     4381 gacgagccag gcat
//
LOCUS       DAORYU010000114         4350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_13557_length_4350_cov_4.250524, whole genome shotgun sequence.
ACCESSION   DAORYU010000114 DAORYU010000000
VERSION     DAORYU010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13557_length_4350_cov_4.250524"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2285
                     /locus_tag="PLW07_04600"
     CDS             <1..2285
                     /locus_tag="PLW07_04600"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lectin like domain-containing protein"
                     /protein_id="HOL49838.1"
                     /translation="SSSYAEGFDRKASDGGNHLMSTAYLVRWSGPIPELEDPYNAGST
                     SSPKNLQPVKHVQEVIFVPDRTSATGNDAIKQAVMKYGAIYTTMYYNNAYLAKGKNYY
                     YNGSATSNHAVSIVGWVDNYSRSNFYGSASGMPPGDGAFIVKNSWGTLWGESGYFYIS
                     YYDSNIGKNCVVFNNAEPVTNYNSIYQYDPLGWVTSAGYNNDTAHFANIFAAESSQLI
                     QAVGFYTPVKNSQYEIKIYKNVSSTPVDGIFATVKTGTIEDPGYHTIKLSTSVVMEKD
                     EKFSAIVRLKTPGYNYPIPVEIPISGYSSKANALPGQSFVSKDGINWSDITMSMPGTN
                     VCIKVYGTGFGLELTSPNGGEMLEQGSTHTIRWNYSGTFSGNLKLELMKNEKLVFVIA
                     SNISKGTGGAGYYNWRIPTTISAGDDYKIRITGTSNSAITDTSDATFSIIAGKITVNH
                     PGSGIKFLKATSQIIQWKTSGNAGGYVKIELLKSNKLVSTIASSVYAVTNGNDSYVWY
                     IPSTIAAGDDYSIRVSSKTNSSIFGISEPFTIIGPSVTLTSPNGGETWYTGTTQKITW
                     TYSGSSPTYLKIECIKGATATTIGQVYCNGSSGYFLWYIPSSMQASQDYKIRLTSISD
                     TSITDTSDDYFTVSAGSIKLVSPYGGEIWNKNETKNIIWTYQGNPGSSVRIELYKGAS
                     FTGLIATKSIGSSGTGYLSWKIPSALKDGSDYKIKVVSASNNSISGESENFSIITGSP
                     GNSFFLNSSQFDETEYNHYR"
     gene            2297..3433
                     /locus_tag="PLW07_04605"
     CDS             2297..3433
                     /locus_tag="PLW07_04605"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HOL49839.1"
                     /translation="MFDNISLIKEGKTGRFSSWDTSGRNGDYWIIPKKSSRVLADIKG
                     PGKITHIWMTQRNHYRECLLKITWDDAEYPSVLVPLGDFFCLGHGIVNSFQSMFFTAS
                     TRNQSTFSGGCALNCYLPMPFKKRALVELINESDEEHFQYFYIDYETFDHDEENAGYL
                     HAEFRRANPFGGWGHEITVNTPEANAVNKEKIAWQNNYVILETKGTGHYIGCNISVTN
                     FQGTWWGEGDDMIWVDGYKWPPDLHGTGSEDYFNQAWGMQKNAFLRNGSSIHEEDTGG
                     YQTSYIFHVENPVRFKKEIKVTIEHGHANHLANEYSSVAYWYAATPTKVQNVPPVEKR
                     LPVMKDHLQRWIHDPLKQITPFKTKMTDEMKKLKKQWKEKDKKK"
     gene            3447..4085
                     /locus_tag="PLW07_04610"
     CDS             3447..4085
                     /locus_tag="PLW07_04610"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOL49840.1"
                     /translation="MISASPRKNKSQTFVLATEVLAGCEPEKTEIIHLCDYKIEFCRH
                     CERCHKKVLDCPIKDDVSRILQKMLGADGIILASPNYINHVTGTMKTLFDRSSHFIHC
                     KRLLNKYVAGVVSSGSGRDASVIDYLKHYALTCGAQFVGGVSSSVPLSEEQKKQAFKL
                     GVKLASAIKNKKIFPSQMKIIEQGKEHFKSIIIARKTDWQEEYDYCKEKKWL"
     gene            4054..>4350
                     /locus_tag="PLW07_04615"
     CDS             4054..>4350
                     /locus_tag="PLW07_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49841.1"
                     /translation="MITVKKKSGSEKLSVKYQRTMLVLGGKYVKKLDYEKLPVLQPSR
                     HIVLSNEHPEWKFQLHNYLIFHENKFWCMWSFGPEEEARIGQSVLYSTSNDGISW"
BASE COUNT         1464 a          798 c          893 g         1195 t
ORIGIN      
        1 tgagttcttc ctatgcagaa ggttttgata gaaaagcatc tgatggtgga aatcatctta
       61 tgtcaacagc atatctggta agatggtcgg gtccaattcc agaactggaa gacccgtata
      121 acgcaggttc cacttcttcg ccaaaaaatc ttcaaccggt gaaacatgtt caggaagtta
      181 tttttgttcc tgacagaaca agcgccacag gtaatgatgc catcaagcaa gcagtcatga
      241 aatatggagc aatttatacc acgatgtatt acaataatgc ataccttgcg aaaggcaaaa
      301 actactatta caatggctct gctacttcaa accatgctgt ttcaattgtc gggtgggttg
      361 acaattatag caggtccaat ttttatggca gtgcttcagg aatgccacct ggtgatggtg
      421 cttttattgt aaaaaatagc tggggaactt tgtggggaga atcaggatat ttttatattt
      481 catattatga ttcaaacatt ggtaaaaact gcgtggtgtt caataacgct gaacctgtta
      541 ctaattataa ctcaatatat cagtatgacc ctcttggatg ggtcacatct gctggataca
      601 acaatgatac tgctcacttt gccaacatat tcgctgctga atcatcacaa ctgatacagg
      661 cagttggatt ttacactcct gtaaaaaata gccagtacga gataaaaatt tataaaaatg
      721 tttcatccac acctgttgat ggaatttttg ccacagtgaa aactggaaca attgaagacc
      781 ctggatacca cacgataaaa ctttcaacca gcgtggttat ggaaaaggat gaaaaattct
      841 ctgccattgt aagattgaaa acacctggct acaactatcc catacctgtg gaaataccta
      901 tttcaggtta tagcagtaaa gcaaatgccc tgccaggaca gagttttgtc agcaaagacg
      961 gtatcaactg gtcagatatt acaatgtcaa tgcctggaac caatgtgtgt atcaaggtat
     1021 atggtacagg ttttggcctg gaattaacaa gtcccaatgg tggtgaaatg ctggaacagg
     1081 gaagcacgca caccatcaga tggaattata gtggaacttt cagtggaaat ttaaaactgg
     1141 aattgatgaa aaacgaaaaa cttgtctttg ttattgcttc taatatatca aaaggaactg
     1201 gcggcgccgg ctattacaat tggagaattc ccacaacgat aagtgctgga gatgactaca
     1261 aaattcgcat cacaggcacg tcaaattccg ctattacaga cacttctgat gcgacatttt
     1321 caattattgc tggaaaaatc acagtgaatc atcccggttc aggaataaaa ttcctaaaag
     1381 caaccagcca gattattcag tggaaaactt ctggaaatgc aggtggttat gtaaaaattg
     1441 aattattgaa gtcaaataaa cttgtcagta ccattgcgtc ttcagtatat gcagttacaa
     1501 atggtaatga ctcgtatgta tggtatattc catcaaccat tgcagctgga gacgattatt
     1561 caataagggt atcaagcaaa acaaactcca gcatttttgg aattagcgaa ccttttacta
     1621 ttattggtcc ttcagttaca ctcacaagtc ccaatggtgg tgaaacgtgg tacactggta
     1681 caacccagaa gattacatgg acatattcgg ggtcgtctcc aacttatctc aagattgagt
     1741 gtataaaggg tgctacagca acaacaatcg gtcaggtata ttgcaatgga agttcaggtt
     1801 attttctctg gtacattcct tcttcaatgc aagcatcgca agattacaaa atcagattaa
     1861 catcaatatc tgacacatct ataacagata caagcgatga ttatttcaca gtttctgctg
     1921 gttcaatcaa actggtctcg ccatatggcg gagaaatctg gaataaaaat gaaacaaaaa
     1981 acatcatctg gacatatcag ggaaatcccg gaagttctgt aagaattgaa ctctataaag
     2041 gagcatcatt tactggctta atagcaacaa aatctattgg ttcttcaggt actggatatc
     2101 tatcatggaa aattccttct gcattaaaag atggctctga ctacaaaata aaagtggtgt
     2161 ctgcttcaaa taacagcatt tcaggtgaaa gcgaaaactt ttcaatcatc actggaagcc
     2221 ctggaaactc atttttcttg aatagttcac agtttgatga aaccgaatac aaccattaca
     2281 gataaggagg gatgttatgt ttgataatat ttctttgata aaagagggaa aaactggaag
     2341 attttcttct tgggatacat caggaagaaa cggtgactac tggattattc ccaaaaaaag
     2401 ttcaagagtt cttgcagata ttaaaggacc aggaaaaatt acccacatat ggatgacaca
     2461 gcgcaatcat tatcgcgaat gtttgttgaa aattacctgg gatgatgcag agtatccttc
     2521 agtgcttgtt ccactcggtg attttttctg tctcggtcat ggaatcgtga attcttttca
     2581 atccatgttt tttaccgctt caacacgaaa ccagagtaca ttcagcggtg gctgtgcctt
     2641 aaactgttat ctgccaatgc ccttcaaaaa acgcgcactt gtggaactta tcaatgaaag
     2701 cgacgaggag cacttccaat acttctacat agattatgaa acctttgatc acgatgaaga
     2761 gaatgcaggc tatctccatg ctgaattcag acgggcaaat ccctttggtg gctggggaca
     2821 cgaaattact gttaatacgc ctgaggcaaa tgctgttaac aaagaaaaaa ttgcctggca
     2881 aaacaattat gtgatacttg aaacaaaagg aaccggccat tatattggtt gtaatatcag
     2941 tgtgacaaac tttcagggta catggtgggg tgaaggtgat gacatgatat gggtggacgg
     3001 atacaaatgg ccacctgatt tgcacggaac aggcagtgaa gattatttca atcaggcgtg
     3061 gggaatgcag aaaaacgcct ttttaagaaa tggctcgtca attcatgaag aagacactgg
     3121 cggataccag acatcatata tttttcatgt tgaaaatcct gtgagattta aaaaagaaat
     3181 caaggtaaca attgaacatg ggcatgccaa tcaccttgcc aatgaatatt cttcagttgc
     3241 ttattggtat gcagcgacac ccacaaaggt tcaaaatgtg ccaccagtgg aaaaaagatt
     3301 gcctgtaatg aaagaccatc tgcagaggtg gatccacgac cctttaaaac aaattactcc
     3361 attcaaaaca aagatgacag atgaaatgaa aaaactgaaa aaacaatgga aggagaaaga
     3421 taagaaaaag tgatttcaat atccttttga tatcagcaag cccaagaaag aacaaaagcc
     3481 agacgtttgt tctggcaaca gaagtacttg ctggttgcga accagaaaaa acagaaatca
     3541 ttcatctgtg tgattataaa attgagtttt gcaggcattg tgaaagatgt cataaaaaag
     3601 tactggattg tccgataaaa gatgatgtaa gcaggatttt gcaaaaaatg ctgggtgccg
     3661 acggaattat tctggctagc cctaactaca tcaatcatgt aacaggaaca atgaaaacat
     3721 tgtttgaccg ctcaagtcat tttatccact gcaaaagact gcttaataag tacgtggcag
     3781 gtgtcgtctc gtcgggtagt ggtagagacg catcagttat tgattatttg aaacattatg
     3841 cccttacctg tggcgctcag tttgttggtg gagtttcttc cagtgttcct ctcagtgaag
     3901 aacaaaagaa acaggcattc aaactcgggg taaaacttgc ctctgcaatc aaaaataaga
     3961 aaatttttcc gtctcaaatg aaaattatcg agcaggggaa agaacatttt aagagcataa
     4021 tcattgcaag aaaaactgac tggcaggaag aatatgatta ctgtaaagaa aaaaagtggc
     4081 tctgaaaaat taagcgtgaa gtatcaaaga accatgcttg tccttggtgg caagtatgta
     4141 aaaaaacttg attatgaaaa attacctgtt ttgcaacctt caagacacat tgttttaagt
     4201 aacgagcatc ctgaatggaa attccaactt cacaattacc ttatttttca tgagaataaa
     4261 ttctggtgta tgtggagttt tggaccagaa gaagaagcac gcataggtca atcagtgctt
     4321 tattccacaa gcaacgatgg catctcctgg
//
LOCUS       DAORYU010000115         4296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_13784_length_4296_cov_5.639236, whole genome shotgun sequence.
ACCESSION   DAORYU010000115 DAORYU010000000
VERSION     DAORYU010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13784_length_4296_cov_5.639236"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="PLW07_04620"
     CDS             <1..606
                     /locus_tag="PLW07_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HOL49842.1"
                     /translation="HQSLFKDGKNAFFDGKDPNYLSSVAKSYTAGLLKHIREITSICN
                     QWVNSYKRLVPGFEAPVYISWGRKNRSALVRVPIYKPGKESASRIELRSPDPACNMYL
                     AFTVILAAGYTGIEEKLKLPEPVEIDIYGMTAEEKTKNSIETLPGSLIEAIHETEKSK
                     LVRDALGEHIFTKFIENKKIEWDKYRICVNQYELENYFPVL"
     gene            612..1133
                     /locus_tag="PLW07_04625"
     CDS             612..1133
                     /locus_tag="PLW07_04625"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOL49843.1"
                     /translation="MQYILDGNNIIKHRLWNQGKKIFDDRLSLIIFLKEYCNKHPSVK
                     FTVVFDGWADISSSHSGIKIIWSHDESADHVIIRKLASARKDSIIVSNDNQVRNGAKF
                     AGFRVFKVEEFLAIFKKRQNGKQSETIAGKEKKIPYSRVPQIQKELEEYYEKHPQETE
                     YVRKLRKRHKRFL"
     gene            1093..1701
                     /locus_tag="PLW07_04630"
     CDS             1093..1701
                     /locus_tag="PLW07_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HOL49844.1"
                     /translation="MLENLEKDIRDFFETEDKIREQTLKLSRDVTRCSARAIKKLHQG
                     DFEESQKLLEQAMSSLVNAKKILAGFPEILYAGFVHNAEKELIEGLVFYRIVKENSLF
                     VPDFDVFDRISYLHGIAEAMGELRRHILDQIRQDNTKNMEQFLSLMDEVYYFSSSFDY
                     PDVITRGLRRTVDVLRAIVEKTRADITLVLQQKKFEKKLENR"
     gene            1698..2486
                     /gene="pgeF"
                     /locus_tag="PLW07_04635"
     CDS             1698..2486
                     /gene="pgeF"
                     /locus_tag="PLW07_04635"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HOL49845.1"
                     /translation="MNYSYFPYLLSFDEIPVFNFCTTRYGGISLSGFETSNLGFNTDD
                     SIERIRENRMLTAARLQIPTDEFILLKQVHGDSVHYISRQHCGKFLDREMMEGDGMFT
                     DIEGLTLAVTLADCAGIVLYEPKKHVAGVCHSGWKGCLLNICGKLVDKMAEKCGCNSG
                     EIVAGISPSICGRCYTVGYEIIQKFPEKYSGFISKYHDGWHLDLEGIVMSQLIEKGVK
                     KEHIFTSGICTYENTPMFYSHRAEKNTGRFIFGIRLLPGRQHEQ"
     gene            2476..3837
                     /locus_tag="PLW07_04640"
     CDS             2476..3837
                     /locus_tag="PLW07_04640"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HOL49846.1"
                     /translation="MNNDFFVRSTPAITIRGIHLDLKGLPPTPSRLVELLELCAEARI
                     NCVLVEWEDMYPWKTYPELQNDTAYSENTVKKFFSKAESLNIEVIPLVQSFGHMENAL
                     SKARFKHLREIPDNVSDLCPLKTGSKEVVLQMVKDVLLTHIGIKHFHLGGDEVWTFGH
                     CPVCKKFIEKHGKASLYLYHLEPLLQFLNEKGIRPIIWDDMIRTWDISDVKNIGKQTD
                     LMCWSYGIDPFKFVTRDTIEKFVKAGCNLWAASAFKGADGAYVDIPDIHARSANMLAW
                     VKYATQIKLKGVVATGWSRYNTFLSPCEGLEASLDTLVLAGKLAWDSKMPDNASEWAD
                     ALLKLCHKKGIKTAHFSECRKSSEQLQSLRNSAFACARNFLQQAHLAGEPERINPFRE
                     KDLKKHFLGSLKNVQKVARQWEKAHRTLVPSVWLKKYTLSRTEPVRKLFDIVNRKSSS
                     GTL"
     gene            3905..4102
                     /locus_tag="PLW07_04645"
     CDS             3905..4102
                     /locus_tag="PLW07_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49847.1"
                     /translation="MKKQQRLLKKYYHRRMQAAKKQLQEFEQGNRPFEKLNRRAKKLL
                     QKRIKAGYELPARLFIRHSTQ"
     gene            4114..4203
                     /locus_tag="PLW07_04650"
     tRNA            4114..4203
                     /locus_tag="PLW07_04650"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4148..4150,aa:Ser,seq:gga)
BASE COUNT         1413 a          816 c          916 g         1151 t
ORIGIN      
        1 caccagtccc ttttcaaaga tggcaaaaac gcattttttg atggaaaaga cccaaattac
       61 ctctcttcag ttgcaaagtc atacactgct ggtcttttga aacacatcag ggaaattaca
      121 tccatatgca atcagtgggt taattcttat aaacggcttg ttcctggttt tgaagcccct
      181 gtgtatattt cctggggaag aaaaaaccgt tcagcccttg ttcgcgttcc catctataaa
      241 ccaggcaagg aaagcgcaag ccgcattgaa ttgagaagcc ctgaccctgc gtgcaatatg
      301 taccttgcct ttacagtcat tcttgccgca ggatatactg gaatagaaga aaaactcaaa
      361 ttgccagaac ctgttgaaat cgacatttat ggaatgactg ctgaagaaaa gacaaaaaac
      421 agtatagaaa ccctgcctgg aagtttgata gaagcgattc atgaaacaga aaagagcaaa
      481 cttgtcagag acgcgcttgg tgaacacata tttacaaaat ttatcgaaaa caagaagatc
      541 gaatgggata aatacaggat atgcgtcaac cagtatgaac ttgaaaacta ctttcctgta
      601 ctctgattta tatgcaatat attctggatg ggaacaacat tatcaagcac agattatgga
      661 atcagggtaa aaaaattttt gatgatagat tgtctctgat tatttttcta aaagaatact
      721 gcaacaagca cccttctgta aaatttacag ttgtttttga cggctgggct gacatctctt
      781 catctcattc gggtatcaaa atcatctggt ctcatgatga atctgctgat catgtcatta
      841 tcagaaaact tgcttccgca agaaaagata gtattatcgt atcaaatgat aatcaggtta
      901 gaaatggcgc aaaattcgct gggttcaggg tttttaaggt tgaggaattc ctcgccattt
      961 tcaaaaaaag acaaaacgga aaacaatccg aaaccatagc cggcaaagag aaaaaaatcc
     1021 cgtacagtcg ggtgccgcag atacagaaag aactggagga atattatgaa aaacatcccc
     1081 aggaaacaga atatgttaga aaacttagaa aaagacataa gagatttctt tgagacagag
     1141 gacaaaataa gggaacagac attgaaactt tccagagatg ttaccaggtg ctctgcccgt
     1201 gcaataaaaa aattgcatca gggagatttt gaagaaagcc aaaaactgct tgaacaagca
     1261 atgtcatccc ttgtcaacgc aaaaaaaatt cttgcagggt ttcctgaaat tttgtatgct
     1321 gggttcgtgc ataatgctga aaaagaattg attgagggac ttgtgttcta cagaatcgtt
     1381 aaggaaaaca gtttatttgt tcctgacttt gatgtgtttg acagaatcag ttatttacac
     1441 ggaattgcag aggcaatggg tgagttgaga agacacatcc ttgatcagat cagacaggat
     1501 aacaccaaaa atatggaaca gttcctttct ttgatggatg aggtgtatta cttttcaagt
     1561 tcctttgact atcctgacgt aattacccga ggattgagaa gaactgttga tgtgctcagg
     1621 gcaattgttg aaaaaacacg ggctgatatt acacttgttt tacagcaaaa aaagtttgaa
     1681 aagaaactgg aaaatcgatg aattattcgt atttcccata ccttctttct tttgacgaaa
     1741 tcccggtttt caacttctgc acaaccagat atgggggaat atccctctcg ggctttgaaa
     1801 caagcaacct gggttttaat actgatgaca gcattgaaag aataagagaa aatagaatgt
     1861 taactgccgc acgattacaa atacccacag acgaatttat attgttgaaa caggtccatg
     1921 gcgatagcgt tcactacata agcaggcaac actgtggaaa atttcttgac agggaaatga
     1981 tggaaggtga cggcatgttc actgatattg aaggactgac ccttgctgtg acccttgcag
     2041 attgcgctgg cattgtactt tatgaaccca aaaaacacgt tgcaggagta tgccatagcg
     2101 gatggaaagg atgccttctg aatatctgtg gaaaacttgt ggataagatg gctgaaaaat
     2161 gtggatgtaa tagtggagaa atcgttgcag gcatcagtcc ttcaatatgt ggacgttgtt
     2221 acacagtggg atatgaaatt attcagaagt ttccagaaaa atacagtggt tttatttcaa
     2281 aatatcatga tgggtggcat cttgatttag aaggtattgt gatgtcgcag ttgattgaaa
     2341 aaggtgtcaa aaaagaacat atatttacct ctggcatctg cacatacgag aataccccca
     2401 tgttttattc gcacagggca gaaaaaaaca caggaagatt tatttttgga attcgtcttt
     2461 tacccgggag gcaacatgaa caatgatttt tttgtccgca gtacaccagc cattaccatc
     2521 agggggatac accttgactt aaaaggactt ccacctaccc cttcaagact tgttgaactc
     2581 cttgaacttt gtgctgaagc aaggataaac tgtgtgcttg ttgaatggga agacatgtat
     2641 ccatggaaaa cgtatccaga actacaaaat gacaccgcct attctgaaaa cactgtaaaa
     2701 aaattttttt caaaagcaga atccctgaac atagaagtaa ttcctcttgt gcagagtttt
     2761 gggcatatgg aaaatgcgct ttcaaaggca cgtttcaaac acctgcgaga aattcctgat
     2821 aatgtcagtg acctttgtcc tctgaaaaca ggttcaaaag aagtagtgtt gcagatggta
     2881 aaagacgtcc ttttaaccca tatcggcatc aaacactttc atcttggcgg agatgaggtc
     2941 tggacctttg gccattgccc cgtctgcaaa aaatttattg aaaaacatgg taaggcatct
     3001 ctttatctgt atcatcttga acccctttta caatttctca atgaaaaagg catcagacca
     3061 ataatctggg acgatatgat aagaacatgg gacatctctg acgtgaaaaa catcggtaaa
     3121 cagacagatt taatgtgctg gagttatgga atagacccgt tcaagtttgt caccagagat
     3181 accattgaaa aatttgtgaa agccggttgc aacctctggg ctgcttctgc attcaagggc
     3241 gcagacggcg catacgttga tattcctgat attcatgcaa gaagtgccaa tatgcttgcc
     3301 tgggtgaaat acgcaacaca gataaaattg aagggagttg ttgcaactgg ctggagcagg
     3361 tacaatacat ttttaagccc ctgtgaaggg cttgaagcaa gccttgatac ccttgtgctt
     3421 gctggaaaac ttgcatggga tagcaagatg ccggacaatg cctctgaatg ggcagatgct
     3481 ttgctgaagt tgtgccacaa aaaaggcata aaaactgctc atttttcaga atgcaggaaa
     3541 tcatcagaac aacttcaatc cttacgcaac tcagcatttg cttgcgcaag aaatttcctt
     3601 cagcaggcgc atctcgctgg agaaccagaa agaatcaacc ctttcagaga aaaggattta
     3661 aaaaaacatt ttcttgggtc attgaaaaat gtgcaaaaag ttgccagaca gtgggaaaaa
     3721 gcccacagaa cccttgttcc ttccgtatgg ctgaaaaaat atacactttc aagaacagaa
     3781 ccagtgagaa aattgtttga cattgttaac agaaagtcgt cttcaggtac attgtgattt
     3841 tgacaccaga cctgctattc aagtaaaata tgttttacaa aaatgcgttt tacaggaggg
     3901 atagatgaag aaacagcagc gacttttgaa aaagtattat cacagaagaa tgcaggcagc
     3961 aaaaaaacag ttacaggaat ttgagcaggg aaatcgtccc tttgaaaaac tcaatcgcag
     4021 ggcaaaaaaa cttcttcaaa aacgaattaa agccggctat gaattaccgg caagactctt
     4081 cataagacat tccacccagt aattctttgt tctggagggg tcgcatagtg gccgagtgcg
     4141 cacgcttgga aagcgtgttc tcgagtaatc gggacgtggg ttcgaatccc accccctccg
     4201 ccagttggga aatgtctttg ttggctcgcc ctctgcgagg actcgccagc cgattttagg
     4261 cgcaaaattg aattttttgt ctttcaaaat aaagtt
//
LOCUS       DAORYU010000116         4293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_13799_length_4293_cov_5.071260, whole genome shotgun sequence.
ACCESSION   DAORYU010000116 DAORYU010000000
VERSION     DAORYU010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13799_length_4293_cov_5.071260"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1112)
                     /locus_tag="PLW07_04655"
     CDS             complement(<1..1112)
                     /locus_tag="PLW07_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOL49848.1"
                     /translation="MGQTIAEKILSSHAGKQVFAGEICLAKVDFLMAQDGTAPLVIKA
                     FEELEGNKIFDPERVCFVFDHSAPSPNMGVSSLHKLMRDFIQKYGIKKMEIGEGVCHV
                     LMPEKGFLRPGQLVLGADSHTPTYGALNLVSCGVGSTDLAIAMLTGKQWFRVPYSTKL
                     VYHGKLKKGVFAKDMGLLMVKTLTAQGAVYQSVEIAGPAIKNMSMDGRFTIANLVTEV
                     GGKNCVMEYDNKTEKFLKGFVGENETINPVFADHDAIYSNIVELDVSKLVPLVAKPHT
                     VDNVSPVEELRGTTINQAFIGTCTNGRLEDLAIAAKILEGRKVHKDVRTIVTPGSRKI
                     YLEAMKKGYIKIFVESGCCVTNPGCGPCVGTHQGVLS"
     gene            complement(1121..2353)
                     /locus_tag="PLW07_04660"
     CDS             complement(1121..2353)
                     /locus_tag="PLW07_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HOL49849.1"
                     /translation="MPKIYIIDVTNRDGVQTARLGLAKLEKTIINMYLNQMGIFQSEA
                     GFPVTKHEINYLNANIALVKKGVLTPIKISGWMRAIVDDVAEAFKNVPGLEHLNLSIS
                     TSQQMILGKFQGKRDFSGVIKDMTSAVDEAKAHGAKSIGVNAEDASRTDMNKLIEFTL
                     AAKEHGASRIRYCDTLGYDTPFTIYERIYQLTSKTKMPIELHCHNDLGMAVACSIAGA
                     KAAIDAGVDAYINTTINGMGERAGNADLISVLLACKYGAGMAGKYQMDERINLAMSWK
                     IAHYASYAFKVPIPINQVGVGANAFAHSSGIHVDGALKDKRNYELYDFEELGRGEPEI
                     IETGRMIILGEYSGIKGLRNIYGKLEVEFKDEEDARKILELARYANVHTQKPLTDDEL
                     LFVARHPEEARLLMTVTP"
     gene            complement(2362..2805)
                     /gene="rplM"
                     /locus_tag="PLW07_04665"
     CDS             complement(2362..2805)
                     /gene="rplM"
                     /locus_tag="PLW07_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019116410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOL49850.1"
                     /translation="MKTHKTPVPDGETKIEKKFYLVDANGKTLGRLASNIATILLGKH
                     KSCWVPYQDTGDYVVVINAEKVKLTGKKMFEKVYYRHSGYLGGLKKETFMSLMQKSPE
                     TVIEHAVKGMLPHNRLGRQLLKKLKVYRGSQHPHQAQIPVVLDIK"
     gene            3039..3917
                     /locus_tag="PLW07_04670"
     CDS             3039..3917
                     /locus_tag="PLW07_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49851.1"
                     /translation="MLSKLATVLTGISIALAELPVNQLWAQQNKTDAIPWGAMYVPVD
                     VMKSYGMTGTFGYYRGTAGARKLLVDLETARRFKIRMIVTMGDVKPSVYADSQGRINM
                     EIVKMELAPFVAIAEQLKPFIEDGTIWGIRFMDEPHDPSGLPRGTVINAQDLGNVMAY
                     LKKVFGKVRVGSTAPASYMVNVPDADWCFGQYCHSTMGRHGIKPVAYIEQDASLAKTK
                     GMCYVASLNASTNPVDNKTLFEVYLAFAGLPDVDFLTSWQWPRGKYHGSFEQRISDPS
                     VKTLVEEIPRKCERNR"
     gene            3948..>4293
                     /locus_tag="PLW07_04675"
     CDS             3948..>4293
                     /locus_tag="PLW07_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49852.1"
                     /translation="MQTGKNIYIPDFRRPIRIPGRVKITHASPSFIFAGQKTTLRLRF
                     FISKDISPCLSVKLQISGGRNNKGDFANIQITSEKQDGFVCVRLKKKNLKISECSGYL
                     GTFIIHIPDTGIK"
BASE COUNT         1194 a          914 c          835 g         1350 t
ORIGIN      
        1 gatagaactc cctgatgtgt tccaacacac ggaccacaac cagggtttgt gacgcaacag
       61 ccactttcaa caaaaatttt gatataaccc ttcttcatcg cctcaaggta tatttttctt
      121 gaaccaggcg ttacaattgt ccgcacatct ttatgaacct ttcttccttc aagaattttt
      181 gcagcaatcg ctaaatcttc cagtcttcca tttgtacagg tacctataaa tgcctgattt
      241 attgttgttc ccctaagttc ttccacaggg ctcacattgt caactgtatg gggtttcgcc
      301 acaagtggaa caagtttact gacatcgagt tcaacgatat tgctgtaaat tgcatcatga
      361 tcggcaaata caggatttat cgtttcgttt tcaccaacaa aacccttcag gaatttttcg
      421 gttttattgt catattccat cacgcagttt tttccgccaa cttctgtaac aagatttgca
      481 attgtaaatc taccatccat tgacatgttt tttattgcag gtcctgcgat ttctacgctt
      541 tgatacactg ctccctgcgc tgtcagtgtt tttaccatca gtagccccat atccttggcg
      601 aacactcctt tttttaattt tccgtgatat acaagttttg tggaataagg aaccctgaac
      661 cattgttttc ctgttagcat cgcgatagca aggtcagtgg aacccactcc acaggacaca
      721 agatttaatg caccataggt cggggtatga ctgtcagcgc caagaaccag ttgtcctggc
      781 cttaaaaatc ctttttcagg catcagaaca tggcatacgc cctctcctat ttccatcttt
      841 tttatgccat atttctggat gaaatctctc atcagtttgt ggagagaaga aacccccata
      901 tttggacttg gtgcgctgtg gtcaaaaaca aaacaaaccc gttcaggatc gaaaatttta
      961 tttccttcaa gttcttcaaa tgctttgatc acgagtggtg ctgttccatc ctgcgccatc
     1021 aggaaatcaa cctttgcaag gcatatttcg cctgcaaaaa cctgtttccc tgcatgagat
     1081 gaaagaattt tttctgctat tgtttgtccc atttttcaga ttatggtgtt acagtcatta
     1141 aaagacgcgc ttcttctgga tgtcttgcaa caaaaagtaa ttcatcgtca gtcaatggtt
     1201 tctgtgtatg gacatttgcg tatctggcaa gttcaagaat ttttcttgca tcttcttcat
     1261 ccttgaattc aacctcaagt ttgccgtaaa tgtttctcag tcctttgatg ccgctatatt
     1321 caccaagaat aatcattcgt ccggtttcaa taatttcagg ttcacccctc ccgagttctt
     1381 caaaatcgta caattcataa ttccttttgt cttttagagc gccatcaaca tgtattcctg
     1441 acgaatgggc aaaagcattg gctccaacgc ccacctggtt aatcggaatg ggaaccttaa
     1501 aagcataaga agcataatgt gcaattttcc atgacattgc aagattgatt ctttcatcca
     1561 tctgatactt acctgccatt ccagcgccat atttgcatgc aagtaacacc gaaatcaggt
     1621 ctgcatttcc tgctctttct cccataccgt tgattgtggt gttaatgtat gcgtcaactc
     1681 ctgcgtctat ggctgctttt gctcctgcga tggaacatgc cacagccatt ccaaggtcgt
     1741 tgtggcaatg taattcaatg ggcatctttg tttttgaagt taactggtaa atacgttcat
     1801 agattgtaaa gggtgtatca tatccgagtg tgtcgcaata tctgattctt gaagcaccgt
     1861 gttccttcgc agcaagggta aactcaataa gtttgttcat gtctgttctt gaagcatctt
     1921 cagcattcac tccaattgat ttcgccccgt gtgcttttgc ttcatcaaca gcagaagtca
     1981 tatccttgat aacccctgaa aaatctcttt ttccctgaaa tttgcccagt atcatttgct
     2041 gggatgttga aattgaaaga ttaagatgtt caagtcctgg aacatttttg aacgcttcag
     2101 caacatcgtc cacaatggct ctcatccagc cagaaatttt tatcggagta agaacacctt
     2161 tttttaccag cgcgatattt gcattgagat aattgatttc gtgttttgtg acaggaaacc
     2221 ctgcctcgct ctgaaatata cccatctgat tcaggtacat attaataatt gtcttttcaa
     2281 gttttgccag gccaagccgt gctgtctgca ctccatccct gtttgttaca tcgataatgt
     2341 aaatttttgg cataaaacct cctattttat gtcaagcact actggtatct gcgcctggtg
     2401 cggatgctgc gaaccacgat aaaccttgag ttttttcagc agttgccttc caaggcggtt
     2461 gtgaggcaac attcctttaa cggcatgttc tattactgtt tcagggcttt tctgcatcag
     2521 tgacataaag gtttcttttt ttaatccacc aagataccct gagtgtctgt aatatacctt
     2581 ttcaaacatt tttttcccgg tcaatttcac cttttcagca ttgatgacaa ccacatagtc
     2641 ccctgtatcc tgatatggga cccagcaact tttatgcttg ccaagcaaaa ttgtggctat
     2701 gtttgatgcg agccgtccaa gggttttccc gtttgcatcc acaagataaa actttttttc
     2761 aatctttgtt tctccatctg gaacaggtgt cttatgagtt ttcatgtttc ctccgtttca
     2821 ctcctgaagt ttggataaaa tagtttgaaa aatgaaccag accaggatgt tattttgcca
     2881 agattataca catttcaggt ctttctgtca aaaaactgtt tccagcggta atttcacttg
     2941 attgaaaata acagcagaaa cagcagaacc tgcttttgac accggaaaaa gaaaatgttt
     3001 ttatacatat ggaaaacatc cggatgtgga taatgagaat gctgagtaaa ttagcaactg
     3061 tattaacagg aatttctatt gcgcttgcgg aattgcctgt aaatcaattg tgggcgcagc
     3121 aaaataagac agatgcaatt ccctggggag caatgtatgt gccagttgat gttatgaaat
     3181 cttatggcat gacaggaacc tttggctatt atcgtggaac agccggagca agaaaattgc
     3241 tcgttgatct ggaaacagcc cggcgtttca aaatcaggat gattgtgaca atgggagatg
     3301 tgaaaccttc agtttacgcc gacagccagg gaaggatcaa tatggagata gtaaaaatgg
     3361 aattagcacc atttgtcgcg attgctgaac aattgaaacc cttcattgag gatggtacaa
     3421 tctggggcat tcgttttatg gacgaaccac atgacccatc tggattgccg cgtggcacag
     3481 tcataaatgc gcaggacctg ggaaatgtca tggcatatct gaaaaaggta tttggaaagg
     3541 ttcgtgtcgg gtctactgca cctgcttcct acatggttaa tgtgccagac gctgattggt
     3601 gttttggtca gtactgccac agcacaatgg gtcgtcatgg gatcaaacct gttgcgtaca
     3661 ttgaacagga tgcatctttg gcaaaaacaa aaggaatgtg ttatgttgca agtttaaatg
     3721 cctccacaaa tcctgttgac aataaaacat tatttgaggt ttaccttgct tttgctggtc
     3781 taccagatgt tgactttctg acatcctggc aatggcccag gggaaaatat cacggttctt
     3841 ttgaacagag aatatccgac ccatctgtga agacccttgt ggaagaaatc ccgcgaaagt
     3901 gtgaaagaaa caggtgaatc agtaaataac aaacaggagt aagaagaatg caaacaggga
     3961 aaaatattta tattcctgat tttagaagac ccataagaat acctggcaga gttaaaatta
     4021 ctcatgccag tccatcgttt atttttgcag gacagaaaac aacattaaga ttacgatttt
     4081 ttatatcaaa ggatatcagt ccctgtttat cagtgaaact tcagatatct ggtggaagaa
     4141 acaacaaagg ggattttgca aacattcaga ttacaagtga gaaacaggac ggatttgtct
     4201 gcgtcagatt gaaaaagaaa aatttaaaga tttcagaatg ttcaggttat cttggaactt
     4261 ttatcataca tattcctgat actggcataa aaa
//
LOCUS       DAORYU010000117         4290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_13814_length_4290_cov_6.413459, whole genome shotgun sequence.
ACCESSION   DAORYU010000117 DAORYU010000000
VERSION     DAORYU010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4290
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13814_length_4290_cov_6.413459"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="PLW07_04680"
     CDS             <1..219
                     /locus_tag="PLW07_04680"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HOL49853.1"
                     /translation="LGLGGYYYAQTTDDKQNGAKVGDGNKGKVFAIGPQVKYDAGKIS
                     FILKYQKEMSVENKPDGSKVWLKIVAPL"
     gene            complement(226..1380)
                     /locus_tag="PLW07_04685"
     CDS             complement(226..1380)
                     /locus_tag="PLW07_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF763 domain-containing protein"
                     /protein_id="HOL49854.1"
                     /translation="MIKRGIATLTLDTGECPRWLFEKMVKLGGQILEIIVQEYGPDEF
                     VARISDPVWFQSLGTVVAFDWNASGLTTILTAALKEAIRGKEKQLGLFVCGGKGKTSL
                     KTPDEIVHWGEKIALPNKNISNLVYSSRITAKVDSSLVQDGYQIYHHAFFFSKNGAWG
                     VIQQGMNPQNSTARRYHWFSDKITDFVCEPHTAIISPLIVPCINLTAKESEKTRKIST
                     ELVQSSFSGLIKDIQILRKYSSKTTKIVAIKSGQQELVFAEFKNTEFYYHPVIEEDFS
                     KSRYLERILAKLCDYKPENYEKLIAIEGVGPKTVRALSLVAELIYNAPPSYIDPARYS
                     FAHGGKDGTPYPVDRITYDNTIEFFRKVINKMKLSAGEKNTILKKLQRFR"
     gene            complement(1377..2018)
                     /locus_tag="PLW07_04690"
     CDS             complement(1377..2018)
                     /locus_tag="PLW07_04690"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOL49855.1"
                     /translation="MAVDFSDRTKNKIRSLYRKLLKKHGSPSDSNQWKFWCKRPKTYY
                     EKEIIIIESILTQRTNWKNVERAVQNLKMSKLASLQSVLKTNNKIIEKLIKSCGFSKQ
                     KAQRLRNVAKFVIESGGIKNLEKMPTNVLREKLLSIDGIGKETADDILLYAFERPVFV
                     IDGYTKRFASNHKLSDRNSYDFLQQLFEQSLKKDYKIYQDYHALIVIELKGTK"
     gene            complement(1996..2763)
                     /locus_tag="PLW07_04695"
     CDS             complement(1996..2763)
                     /locus_tag="PLW07_04695"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019867146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HOL49856.1"
                     /translation="MKSNSFNLEGKVALVTGSGTGIGRAIAIKLAEHGACVGIHYNTS
                     YQEALQTLEAIKSMNRKAILLQADLTCEQQANKIVDKIVAEFGKLDILVNNAGSPVQR
                     SKIEECPLTLWNQVFNVNMTSAFMVTKRAIPHLRKTGKGSIVNIISLSVQTGGANGAG
                     PYAAAKGALLVFTRTLARELAPEIRTNAVCPGVIETRHHEIFSTPERMQQYKEETPMK
                     RNGIPEEVANAVVYLVSDAASFTNGALIDVNGGRFLR"
     gene            complement(2760..3866)
                     /locus_tag="PLW07_04700"
     CDS             complement(2760..3866)
                     /locus_tag="PLW07_04700"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOL49857.1"
                     /translation="MTSRQRCRIAMKGGIPDCVPVIPQICHPHAIRALGFDFRKTMIQ
                     AVQNPYLVNELQIECARFYGVDGVRVWIHHQGINKLYDNGENVWQIDRLTKRAIARLD
                     FKGGGWMIPLEEQVIVKNEQDIEKIPVIPAETLLKDEAYKKTGKLIENAKKDLFVITS
                     PGGISVEYATTVRGKSQTMIDLIENPQFIKKILDRATRVAIEKAVAMLKLGVDAFMLG
                     ETFGGVIGPELFKEFCVPYFKMFVDRIKKYDVCIYLHVCGNSTQLFELMAETGVDCIE
                     PLDPLGGVQVKDAKTRLLGKTAIMGGVNTLKLAHGTLDEVKQDIKRCLSEGAWQGGYI
                     LACGDMLPTETSPEKVFAMVQAAHFYQYQQGGKQ"
     gene            3959..>4290
                     /locus_tag="PLW07_04705"
     CDS             3959..>4290
                     /locus_tag="PLW07_04705"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOL49858.1"
                     /translation="MADVFFASAKVRKLERNATLPAKFLRMLDKLEIDSRVRDKTVII
                     KMHMGAGYGFTTIHPLFVRMLVDYVKEHRGTPYITDIVMPDAKRGYTEEVMGCKFIPA
                     TGLRDTDY"
BASE COUNT         1160 a          878 c          799 g         1453 t
ORIGIN      
        1 ctcggacttg gtggatatta ttatgctcaa accacagatg acaaacaaaa tggtgcaaaa
       61 gtgggagacg gaaacaaagg aaaggtattt gccattggac cacaggtgaa atacgatgca
      121 ggaaaaatta gttttatcct gaaatatcag aaggaaatgt ctgttgaaaa taaacctgat
      181 ggtagtaagg tttggctaaa aattgtggca cccttgtgag gtttttcaac ggaatcgttg
      241 aagttttttt agtattgtgt ttttttcacc tgctgaaagt ttcattttat tgataacttt
      301 tctgaagaac tcaatggtgt tgtcgtatgt aatacggtca acagggtagg gtgttccatc
      361 ttttcctcca tgggcaaatg aatatcttgc aggatcaata tacgagggtg gggcattata
      421 aatcaattct gcaacaagag aaagggctct aactgttttc ggcccgactc cttcaattgc
      481 tatgagtttc tcataatttt ctggtttata atcgcataat tttgcaagaa ttctttcaag
      541 atacctgctc tttgaaaagt cctcttcaat tactggatga taataaaact cagtgttttt
      601 gaattctgca aaaaccagct cctgttgtcc ggatttaatg gcaactattt tcgttgtctt
      661 gctggaatat tttctcagta tctgtatatc cttgattaat ccagaaaaac ttgactgaac
      721 aagttctgtg ctgatttttc tcgttttttc actttctttt gctgtaagat taatgcaggg
      781 gacaattaaa ggactgataa tcgcagtatg tggctcacaa acgaaatcag tgattttatc
      841 agaaaaccaa tgatatcttc tcgcagttga attttgcggg ttcattccct gttgaatcac
      901 tccccatgcg ccattttttg agaaaaagaa ggcatggtga taaatctgat atccatcctg
      961 aacaagactg ctatcaactt ttgctgtaat tctggaacta taaacaagat ttgaaatgtt
     1021 tttgttgggc aatgcaattt tttctcccca gtgaacgatt tcgtcgggtg ttttgagtga
     1081 cgttttaccc ttaccgccac agacaaaaag ccccaactgc ttttcttttc ctctgatggc
     1141 ttcttttaat gcagccgtga gaatcgttgt tagtcctgat gcattccaat caaaggcgac
     1201 aacagtcccc agcgattgaa accaaacagg gtcagagatt cttgcaacaa attcgtcggg
     1261 tccatattcc tgcacaatga tttccagaat ctggcctcca agttttacca ttttttcaaa
     1321 aagccatcgt gggcactcgc ctgtatcaag tgtcaatgtt gcaattcccc tttttatcat
     1381 tttgttcctt tcagttcaat cacaatgagc gcgtgataat cctgatatat tttataatcc
     1441 tttttcaatg attgttcaaa tagttgctga agaaaatcat atgaatttct gtcagacaat
     1501 ttgtgattgc ttgcaaacct ttttgtataa ccgtcaataa caaacacggg tctttcaaat
     1561 gcataaagca aaatatcgtc tgcggtttcc ttaccaatac catcaatgga aagtagtttt
     1621 tctctcaaaa catttgtcgg cattttttca agattcttta tgccgccaga ttcaataaca
     1681 aattttgcga catttctcaa tcgctgtgct ttttgtttgc taaaaccaca gctttttatc
     1741 agtttttcaa tgattttatt gtttgttttc aacacagatt gaagtgatgc aagtttgctc
     1801 atcttcagat tttgtactgc cctttctaca tttttccagt ttgttctctg ggtgagaatt
     1861 gactcaataa taatgatttc tttttcatag tatgtttttg gccttttgca ccagaatttc
     1921 cactgattgg aatcagacgg agaaccgtgc tttttcaaca gttttctgta cagagacctg
     1981 attttattct ttgttctatc tgagaaatct accgccattg acatctatca gtgcaccatt
     2041 ggtaaacgac gctgcatcac tgacaaggta aaccacagca tttgccactt cttctggaat
     2101 cccatttctt ttcatggggg tttcctcttt gtattgttgc attctttctg gagtactaaa
     2161 aatttcatga tgacgggttt caatcactcc tggacagact gcgtttgtcc ttatttcagg
     2221 tgcaagttct cttgctaatg ttcttgtgaa aaccagcagg gcaccttttg ctgctgcata
     2281 cggtccagcg ccatttgcgc cgcctgtttg cacagaaaga gaaatgatgt tgacaatgga
     2341 acccttaccg gtttttctga ggtgcggaat tgctcttttt gtgaccataa atgccgatgt
     2401 catatttacg ttaaagacct gattccatag tgtcagtggg cattcctcaa ttttgcttct
     2461 ttgtacagga gaaccggcat tattgacaag aatatcaagt ttaccaaatt cagcaacaat
     2521 tttgtcaact attttgtttg cctgttgttc acaagtaagg tctgcctgta gtaaaattgc
     2581 cttcctgttc atacttttga tggcttccaa tgtctgaagt gcttcctggt aactggtgtt
     2641 gtagtgaatg ccaacacatg ctccgtgttc tgcaagtttt attgctattg ctctgccaat
     2701 gccagtacct gagccggtta cgagagccac ctttccttca agattgaatg aattgctttt
     2761 cactgtttac ctccctgctg atattgataa aagtgtgcgg cctgtaccat tgcaaaaacc
     2821 ttttccggag atgtctctgt tggaagcatg tcgccacaag caagaatgta tccaccctgc
     2881 caggcacctt cagagaggca tcttttgata tcctgtttta cctcgtccag tgttccatga
     2941 gcaagtttca aagtatttac cccacccata attgctgttt tacccagcaa ccttgttttt
     3001 gcgtctttta cctgtactcc tcccaatggg tcaaggggtt ctatacaatc cacacctgtt
     3061 tctgccataa gttcgaaaag ctgggtactg tttccacaaa catgcagata aatgcaaaca
     3121 tcgtattttt taattctatc cacaaacatt ttgaagtatg gtacacagaa ttctttgaaa
     3181 agttcaggac ctatcactcc accaaatgtt tctccaagca tgaatgcatc gactccaagt
     3241 ttaagcattg caactgcttt ttcaattgca actctggtcg cgcggtcaag tattttcttt
     3301 ataaattgtg gattttcaat aagatcaatc atagtttgag atttgcctct tactgttgtg
     3361 gcatattcta cgcttatccc gccaggcgaa gtgatcacaa acaaatcttt tttagcgttt
     3421 tcaataagtt ttcctgtttt tttatatgct tcatctttca gaagtgtttc tgccgggatt
     3481 accggaattt tttcaatatc ctgctcattt ttaacaataa cttgttcctc cagtggtatc
     3541 atccagccgc ctcccttaaa gtcaagtctt gcaatggctc ttttagttaa ccggtcgatt
     3601 tgccagacat tttcaccatt atcgtaaagc ttgttaatgc cctgatgatg tatccagacc
     3661 cttacaccat caacaccgta aaatcgtgca cattcaattt gaagttcatt aacaagatac
     3721 gggttctgga ctgcctgtat catagttttt ctaaaatcaa aaccaagagc tctaatggcg
     3781 tgtggatgac atatctgtgg tattacaggt acgcaatcag gtatgccacc tttcattgca
     3841 atcctgcacc gctgtcttga tgtcatagga ttctctttgt ttgaaagaaa tttactttca
     3901 tttataatat acctataatt caaggtggtc aaaattttcg tcacaacagg aaagagcaat
     3961 ggcagacgtt tttttcgcct cggcaaaagt acgaaaactg gagagaaatg ctacgcttcc
     4021 tgcaaaattt ctcaggatgc ttgataaact tgaaattgat tctcgagtca gggataagac
     4081 tgttattatt aaaatgcaca tgggagcagg ttatggattt acaacaattc atccactttt
     4141 tgtaagaatg cttgttgact acgtgaaaga acacagagga acaccttata tcacagatat
     4201 tgttatgcca gatgcaaaaa ggggctacac tgaagaggta atgggatgca aatttattcc
     4261 agcaaccggg cttcgtgata ctgattattt
//
LOCUS       DAORYU010000118         4274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_13869_length_4274_cov_4.085802, whole genome shotgun sequence.
ACCESSION   DAORYU010000118 DAORYU010000000
VERSION     DAORYU010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13869_length_4274_cov_4.085802"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="PLW07_04710"
     CDS             <1..473
                     /locus_tag="PLW07_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49859.1"
                     /translation="VFTILLVMVAVCQGQSISALVSNPDLFDGKVVIVDGEFVGDIIE
                     GKDGFWANLLESDVAIGIWCPLSEREKIKFLGKYGVEGDTVRIRGIFHKICVEHTGDM
                     DIHASSIEVLQQGRIREEEIPMEKVIFAFSLGIVSLAAIGLLHILTRRESPHGD"
     gene            complement(382..1731)
                     /gene="miaB"
                     /locus_tag="PLW07_04715"
     CDS             complement(382..1731)
                     /gene="miaB"
                     /locus_tag="PLW07_04715"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOL49860.1"
                     /translation="MKKSFLIKTYGCQMNFAESDRLRKLLVKSGWNESASIKDAGLVI
                     AVGCSVRQKAENRVLSFLKSNKHLKKNGALLGLVGCTANLHGEKILKQLPFLDIVCGP
                     NHLASIIDNLSGFTGQQIIVRTGESDDPFLEYIPSNNEITMDVPITKGCNNFCSYCIV
                     PFSRGKLKSRKPESILQEIKCAVEKGIKSVTLLGQNVNEYGKDFTNGYDFADLVREIA
                     NIDGLLRIGFLTSHPEDTGEKILKTMAECSKVLKHLHIPVQSGSDKILKKMNRKYTVE
                     KFLEVVEMARDMMPEISITSDIIVGFPEETEHDFEQTYELVKKTRFSELFIFKYSPRP
                     MTKAYNMKDDVSKQEKERRHKIILDMQKKISNEILSSFAGKTCQVLAEKISVKNSSQI
                     IGKNIQGISVAFQGDKNILGTIARIKIVNHREGILCGSICEAALSPQDLQCPGKTQK"
     gene            complement(1742..2005)
                     /locus_tag="PLW07_04720"
     CDS             complement(1742..2005)
                     /locus_tag="PLW07_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49861.1"
                     /translation="MRYFLILFLSFLIGTTVGEILTLLLPESFTNLPFFAPGVVSGFD
                     TVSLNLKVINVNVGIKILTNNFSWIGLLISTTVLLIKELAGSQ"
     gene            2117..3241
                     /locus_tag="PLW07_04725"
     CDS             2117..3241
                     /locus_tag="PLW07_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49862.1"
                     /translation="MKIKSLTPENAICFFGYYDKNQISPSGKHFLFLRVDFQNREPDE
                     NDKAEIFVGEIEKGKYEKIDETYAWNFQQGCMLGWLSEEEIIYNVRIKGEFFSKIHNI
                     RKGIYKILGRPVSCFYPEKKIALSLNFSRLAKWRPGYGYQGVKDRFENEKWPEKDGIY
                     FIDLETGQNRLIVPLSKMLEFRKEKGIPDSYGWFNHTLFSRDGKRFCFVNRWKERPEQ
                     THRTRFMTSDIEEREIYDLVESYLISHFDWKNGKEILAWAEIQGHRAFYLIEDKTGRY
                     RKISDRMQTGDGHCSFSADRNRILYDTYPIEGYRYLKIFDTQKDQETVLGKFCSIPEI
                     TGPIRCDLHPRWESENAITIDSVHEGYRRCYLIKFGSLFT"
     gene            3330..>4274
                     /locus_tag="PLW07_04730"
     CDS             3330..>4274
                     /locus_tag="PLW07_04730"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOL49863.1"
                     /translation="MSFLTTLRALQNRNYRLFFFGNGLSLIGTWVQAVTVGWLVYRLS
                     HSSYMLGIAGALGTLPILLFSIFGGYVADRVNRFKILLTTQTFAMIQAFTLAILTLTG
                     HIKVGHVFFLSFVLGAINAFDIPARQAFVVNIVGKKEDLPNAIALNSFLVNGARLIGP
                     SIAGVLIAAVGEGICFLINAVSYIAVIIALLMMHTPEIVVGNTITDSENMMKKLQEGF
                     KYVKTFAPVRTIILYVAFFSIFGMFYGVLMPVFAKEVFRGNARTLGFLVGSGGIGAIV
                     GAIFLASRRVHSGLGRVIHFAGLVFGVCLILFSHCKTFW"
BASE COUNT         1255 a          719 c          888 g         1412 t
ORIGIN      
        1 ttgtttttac cattttgctg gttatggttg ctgtttgcca gggtcagagt atttctgccc
       61 tggtcagcaa tccggatttg tttgatggaa aagttgtgat tgttgatggt gaatttgttg
      121 gagatattat tgagggtaaa gatggtttct gggcaaattt gcttgaatca gatgtggcga
      181 ttggtatatg gtgtccactg agtgagagag aaaaaattaa atttcttggc aaatatggtg
      241 ttgaaggaga tactgttaga attagaggca tatttcataa aatatgtgtg gaacatacag
      301 gagacatgga tattcatgcc agttcaattg aggttttgca acagggcagg ataagggaag
      361 aagagatacc gatggaaaaa gttatttttg cgttttccct gggcattgta agtcttgcgg
      421 cgatagggct gcttcacata ttgacccgca gagaatcccc tcacggtgat tgacaatttt
      481 gattcttgca attgttccga gaatattttt atcaccttga aacgcaacgg agattccctg
      541 aatatttttc ccgattattt gtgatgaatt tttgacactg attttttcag ccagtacctg
      601 gcaagttttt ccagcaaagg aagaaagaat ttcattgcta atttttttct gcatgtcaag
      661 aataatttta tgcctgcgtt ccttttcctg ttttgataca tcatctttca tattgtatgc
      721 ctttgtcatt ggtcttggag agtatttgaa aataaatagt tcactaaacc gtgttttttt
      781 aacaagttca tatgtttgtt caaaatcatg ttctgtttct tcgggaaaac ctactatgat
      841 gtcagacgtg atagaaattt ctggcatcat gtctcttgcc atttcgacga cctcaaggaa
      901 tttttccact gtatattttc tgttcatctt tttcaagatt ttgtcagaac cactttgtac
      961 tggtatgtgc agatgtttga gaactttgga acattcagcc attgtcttca aaattttttc
     1021 ccctgtatct tctgggtggg atgttagaaa cccgattctc aaaagtccat ctatatttgc
     1081 aatctccctg acaagatcag cgaaatcata gccattagta aaatcctttc catattcatt
     1141 gacattctgc cccagaagag ttactgattt aatccctttt tcaactgcgc atttgatttc
     1201 ctgtaaaatt gattctggtt ttctactttt aagttttcct cttgaaaaag gaacgatgca
     1261 gtatgagcaa aagttgttac atccctttgt gatgggaaca tccattgtaa tttcgttgtt
     1321 agaaggaata tactcaagga atgggtcgtc gctttcgcct gttctgacaa taatttgctg
     1381 tcctgtaaaa ccgctaaggt tatcaatgat acttgcaagg tgatttggtc cgcacacaat
     1441 atccagaaac ggaagttgtt tcaatatttt ttcaccatga agatttgcag tacagccgac
     1501 cagtcccagc agggctccat tttttttcag gtgtttatta cttttgagaa aagagagtac
     1561 tctattttcg gctttttgcc tgacgctgca accaacagca attaccagcc ctgcatcttt
     1621 tatgcttgct gattcattcc agccagattt tacaagcaat ttcctcaatc tgtcagattc
     1681 agcaaaattc atctggcatc cataggtttt gatgagaaaa ctttttttca ttacagaata
     1741 gttattgtga accagccagt tcttttataa gcaatacagt ggttgaaatc agaagcccta
     1801 tccaggaaaa attatttgtg agaattttta tacctacgtt aacattgata accttcagat
     1861 ttaaacttac cgtgtcaaag cctgatacaa cgcccggcgc aaaaaatggg agatttgtaa
     1921 aactctctgg cagtaataat gttagtatct cgccaactgt cgtaccgata aggaaactca
     1981 agaacaaaat cagaaaatat ctcatcaatt tcatcaaaat attctacttt tttattttca
     2041 ctgcgtcaaa accaattgtt gtgtttctgg acttgttgcg tgtatgtggt aaaatccaac
     2101 aataaccgga agatctatga aaataaaatc gttaacacct gagaatgcaa tatgtttttt
     2161 tggttattac gataagaacc agataagccc atctggtaaa cattttctat ttttgagggt
     2221 tgattttcag aatagggaac cagacgagaa tgataaggca gaaatatttg ttggtgagat
     2281 tgaaaaagga aaatatgaaa aaatagacga gacatatgcc tggaattttc agcagggatg
     2341 tatgcttgga tggttgtcag aagaagagat tatttataat gttaggataa aaggggaatt
     2401 tttttcgaaa atccacaaca taagaaaagg catttacaaa atattgggaa gacctgtaag
     2461 ttgtttttat cctgaaaaaa aaatcgcctt gagtttaaat ttttcaagac ttgcaaaatg
     2521 gagacccgga tacggatatc aaggagtaaa agacagattt gaaaatgaaa aatggcctga
     2581 aaaagatggc atctatttca ttgatttaga aaccggtcaa aacaggttga tagtgccact
     2641 ttcaaaaatg cttgagttca gaaaagaaaa gggaattcca gattcgtatg gatggttcaa
     2701 tcataccctg ttcagcagag atggaaaaag attttgtttt gtgaacaggt ggaaagaaag
     2761 acctgaacaa actcatagaa caagatttat gacatccgac atagaagaac gagaaatata
     2821 cgatctggtt gaatcgtatc ttatttcgca ctttgactgg aaaaatggaa aagaaatact
     2881 ggcatgggct gaaatacagg gacatagagc attctatctt attgaggata aaacagggag
     2941 atacagaaaa ataagtgata gaatgcaaac aggagatgga cactgttcat tttcagcaga
     3001 caggaatcgt attctttacg atacatatcc catcgaagga taccgctatc ttaaaatttt
     3061 tgacacacag aaagaccagg aaactgttct gggaaaattc tgttcaatac ctgaaataac
     3121 cggaccaatc agatgtgatt tacacccaag gtgggaatct gagaatgcaa ttaccataga
     3181 ttcagtacac gaaggttatc gaaggtgtta tctcataaaa ttcggttcac tgtttacttg
     3241 agagattttt tgatcgtttg atagcagtat gattatgtga tatatattac cgaaaattca
     3301 tgtgtaatcc agtataatcg gaggtaatgg tgtctttttt aacaactctc agggctctgc
     3361 aaaatcggaa ttatcggctc tttttctttg gcaatgggct ttctttaata ggtacctggg
     3421 ttcaagccgt tactgtgggc tggcttgtgt acagattgag ccattcttca tacatgcttg
     3481 gaatagcagg tgcacttggt acattgccca ttctgttgtt ttccattttt ggtggctatg
     3541 ttgctgacag ggtgaacaga ttcaaaattc ttcttacaac tcaaactttt gcaatgatac
     3601 aggcattcac acttgcgata ctcaccctta ctgggcatat caaagttggt catgttttct
     3661 tcctttcatt tgtccttggc gcaataaatg cttttgacat tcctgcaaga caggcgtttg
     3721 tcgtgaacat tgtgggaaag aaagaagacc ttccaaatgc tatcgcattg aattcttttc
     3781 ttgtcaacgg tgcgcggttg ataggccctt caatagcagg tgtgcttatt gctgctgttg
     3841 gtgaaggtat ttgtttcctt attaatgcgg tcagttacat tgctgttatt attgctcttt
     3901 tgatgatgca tacgccggag attgtagttg gaaatactat tacggattcg gaaaacatga
     3961 tgaagaagtt acaggaaggt tttaaatacg tgaaaacctt tgcaccggtg cgcacaataa
     4021 ttctgtatgt cgcatttttc agcatatttg gaatgtttta tggagtgttg atgcctgtat
     4081 ttgctaaaga agtatttcga ggcaatgcca gaacgctggg ttttctggtt ggaagcggtg
     4141 gaattggagc aatcgttgga gcaatttttc ttgcttcacg tcgcgttcat tctggacttg
     4201 gtagagtcat tcattttgca ggacttgttt ttggtgtatg tctgattcta ttttctcatt
     4261 gtaaaacttt ctgg
//
LOCUS       DAORYU010000119         4236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_14029_length_4236_cov_7.394642, whole genome shotgun sequence.
ACCESSION   DAORYU010000119 DAORYU010000000
VERSION     DAORYU010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14029_length_4236_cov_7.394642"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="PLW07_04735"
     CDS             complement(<1..298)
                     /locus_tag="PLW07_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018707521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1646 family protein"
                     /protein_id="HOL49864.1"
                     /translation="MFITGQIIIILLVLVLPLIVHKVEKNLEAFLFIMGIISATISHF
                     FGPEHVWSLHLVKEALIEPIKITLAVFIAGFLFKKFRQSITQGIAKSEQLLGPHI"
     gene            complement(316..1485)
                     /locus_tag="PLW07_04740"
     CDS             complement(316..1485)
                     /locus_tag="PLW07_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49865.1"
                     /translation="MSSKKGADMWTPECWYHDAKNWAQSHKDYMLKMYRINLKLLENH
                     LPGTDEREIHNLLKEYNQSRINAPVDFKKYPELIGMKQLICAEWKGLQDGSGLDEYQT
                     AVYVGAMNFYHRMICSGKMGKMDGSLSSNCSYIYFPYSDHGVLLANNLDSSPDEPFDS
                     PQWPALNEHVIIGSVSSGIYLDEQPEEMFPAPVFKIVARYCRNVKEAVEVLTRYNQFW
                     GPCNAIVVDRNHNCAMIEKSACRIGVRWSKDGFGFITAMTAEEPGMQAYLNDRRQASL
                     KARHLGPDSPDAIYWKKQDQRRQLMNELLDQVRKNPTLETMRKFIQFRDPVRGNVCGN
                     GEIYGPGCPPSEYTIRTVIWLLSEARALWWAKQGDKPSFENPMSEVKFNNVIVWE"
     gene            complement(1498..4014)
                     /locus_tag="PLW07_04745"
     CDS             complement(1498..4014)
                     /locus_tag="PLW07_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49866.1"
                     /translation="MKKKIFLFGILALLSVFAATGQQQSHTAPFKYVYAKAYHVLPGT
                     HNNESGYFSLCEGLDGKIYIGTAKYNENSYLVEFDPYTESQKIVIDTHRVCGLNATGY
                     AAQAKIHTKNFVGSSGKIYVGSKQGYRSPGDTSEYPGGYVMAYDPRTQKAESLGMPYP
                     GEGVIDVVADEQRHLIYVVTCENQHWMLYDEATKKYRDLGVLLLPYATTIIDKNGRAH
                     AITNDFKIATYDPAKDTISIRPVIAGRKILTKPQGKGYAICFWVPGPDGKTAYMTMLS
                     WPDLYEIDLGSSGNTVRAKHLGKMIEGKNPDSRGSLCLHPDGNIYCLWRIDNDTGFGS
                     GYLHHLVRYNVKKKKMEDLGVIAVKNPSFYQFQNPDGTPKVFSHGFHKLPDGTLTILG
                     VHMAMIACRDGTLYATTLYPFTLLKIDDFKLPQKTLTPEDRGYAATQYCKAVLDVCDM
                     VESNLSRISEVAEIVAERHMKGGLIGFYPIVYQGLQDELWGRSGSMVNMGFDRPFKRD
                     RTEEEKKMDVSIIGWQTKPITRDEAKRIEAFKAKGGFVIGFGPKDLPELAEQVRACDV
                     WFDTGTGTDDRCVVLVDGSKAGRTNHLINALNAWALVAEIFSAITRKGHTPAMWKSYA
                     YKDGPGWGNKYMYKKQFMDEYPVNPIPPGTLAKAFLDGIRYHVRKFQNTQLSNIEKAD
                     DLIEKELKKTGKITIASMGHMPWTYVGKYEDAKWAVNADLHSNIPHQVENYLKNTPDG
                     GFVVRLGYTGLDPDSKEIFKKKKQRLIVISAENDPFDFPDWNIPEDVAVYIDMGYAFG
                     DACVSVEGLPIRILPPSGIMQIVAYECLNVEVLSKLANKK"
     gene            4146..>4236
                     /locus_tag="PLW07_04750"
     CDS             4146..>4236
                     /locus_tag="PLW07_04750"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOL49867.1"
                     /translation="MKRKGFTLIELLVVIAIIAVLAAMLLPALS"
BASE COUNT         1163 a          945 c          760 g         1368 t
ORIGIN      
        1 aaatatgggg accaagcaat tgttcacttt tcgcaatacc ctgcgtgatt gattgcctga
       61 attttttgaa caaaaagcca gcaataaata ctgccagagt aatttttatt ggttcaatca
      121 gggcttcttt aacaaggtga agcgaccaga catgctctgg accaaaaaaa tgagaaatgg
      181 ttgctgaaat aattcccatt ataaaaagaa atgcctcaag atttttctca accttgtgaa
      241 caataagagg cagcaccaat accagcaaaa taatgatgat ttgccctgtg ataaacattg
      301 attttttccc ttctgttatt cccagacaat gacattatta aattttactt cagacatggg
      361 attttcaaat gagggtttgt ctccctgttt tgcccaccag agcgctcttg cttcgctcaa
      421 aagccatatc actgttctta ttgtgtattc agatggcggg catccaggtc cataaatttc
      481 tccatttccg cagacatttc ctcttacagg atccctgaac tggataaatt ttctcatggt
      541 ttcaagcgtt ggattttttc tcacctgatc aagaagttcg ttcattagtt gtcttctctg
      601 gtcctgtttc ttccagtaaa ttgcgtcagg actatctggc cctaagtgtc gcgctttcaa
      661 tgatgcctgg cgtctgtcat tcaggtatgc ctgcatgcct ggttcttctg ctgtcatggc
      721 agtaataaat ccaaatccat cctttgacca tctgactcca attctgcacg cacttttttc
      781 tatcatcgcg cagttgtggt ttctgtcaac aacaattgca ttgcatggac cccagaactg
      841 attatatctt gttaaaacct ccactgcttc tttcacgttt ctgcaatatc tggcaacgat
      901 tttgaatact ggcgctggaa acatttcctc tggttgttcg tcaagataaa tccctgaaga
      961 cacagaacca ataatcacat gttcattaag tgctggccac tggggagaat caaatggttc
     1021 atcaggactt gagtcaaggt tgtttgcaag aagtactcca tggtcactat aaggaaaata
     1081 gatataactg cagtttgatg aaagagagcc atccattttt cccatttttc cactgcaaat
     1141 cattctgtga tagaaattca ttgctccaac ataaacagca gtttgatatt catcaagacc
     1201 tgaaccgtcc tgcaaacctt tccattctgc gcaaattaac tgtttcattc cgataagttc
     1261 aggatatttt ttgaaatcaa ccggcgcatt aattcttgat tggttgtatt ctttcaataa
     1321 attgtgaatt tcacgctcgt ctgtacctgg aagatgattt tccagcagtt tcaagttgat
     1381 tctgtacatt ttcagcatat aatccttgtg agattgcgcc cagtttttcg catcatgata
     1441 ccagcattca ggtgtccaca tgtctgcccc ttttttggat gacattatac cacatgacta
     1501 ctttttgttt gccaattttg aaagaacttc aacattcagg cattcatacg caacaatttg
     1561 cataattcca cttggcggaa gtatccttat tggaagccct tcaactgaaa cgcaggcatc
     1621 cccaaaggca tagcccatgt ctatgtaaac ggccacgtct tctggaatat tccagtcagg
     1681 aaaatcaaat gggtcgtttt ctgcgctgat aacaatcaat ctctgttttt tctttttaaa
     1741 aatttcttta gaatcagggt caagtcctgt ataaccaagc cgcactacaa atccaccatc
     1801 aggcgtattt ttcaaataat tttcgacctg atgagggata ttgctgtgaa ggtcagcatt
     1861 gactgcccat tttgcgtctt catatttacc tacatatgtc cagggcatat gtcccattga
     1921 tgcgattgta attttgcctg ttttctttaa ctctttttca ataaggtcat ctgccttttc
     1981 gatatttgac agttgagtat tttgaaattt cctcacatga tacctgattc catcaagaaa
     2041 tgcttttgca agcgtgcctg gtggtattgg attgacagga tattcatcca tgaattgttt
     2101 tttgtacata tatttatttc cccatcctgg accatctttg taagcatatg acttccacat
     2161 cgctggggta tgaccttttc ttgtaattgc agagaaaatt tcagccacaa gcgcccaggc
     2221 gtttaaagca tttatcaggt gatttgttct tcctgccttt gaaccatcaa caagtaccac
     2281 acacctatca tcagtgcctg tgcctgtgtc aaaccacaca tcacatgctc taacctgttc
     2341 agcaagttca ggcaaatcct ttggcccaaa tccaatgaca aatccgcctt ttgctttgaa
     2401 tgcttcaatt ctttttgctt catccctggt gatgggtttt gtctgccagc caataataga
     2461 aacatccatc tttttttctt cttcagttct atccctttta aatggcctat caaaacccat
     2521 gttcaccata ctgccactgc gaccccacag ttcatcctga agtccctgat aaacaatggg
     2581 ataaaaacca ataagacccc ccttcatatg cctttcagca acgatttcag caacttcact
     2641 aattcgagag agatttgatt caaccatatc acagacatca agcactgcct tacagtactg
     2701 agttgctgca tatcctctgt cttctggtgt aagtgttttc tgtggaagtt taaagtcatc
     2761 gattttcagt aaagtaaatg ggtaaagggt tgttgcatac aatgtaccat cccgacatgc
     2821 aatcattgcc atgtgaacac caagtattgt cagggtacca tcaggaagtt tgtgaaatcc
     2881 atgtgaaaaa acctttgggg taccgtcggg attttgaaac tggtaaaacg aaggattctt
     2941 tactgcaatc actccaaggt cttccatttt tttctttttc acattgtatc tgacaagatg
     3001 atggaggtat ccagaaccaa aaccagtgtc attgtcaatt ctccacagac aataaatatt
     3061 tccatcagga tgaagacata aagaaccccg cgaatcagga ttttttcctt caatcatttt
     3121 gccaagatgt ttcgctctca ctgtatttcc agaacttcca aggtcaattt cataaaggtc
     3181 cggccagctc agcattgtca tataggcagt ttttccatca ggcccaggaa cccagaagca
     3241 aatcgcgtaa cctttgccct gaggtttggt aagaattttc cttcccgcaa tcaccggtct
     3301 gattgaaatg gtgtcttttg cagggtcata ggttgcgatt ttaaaatcat tggtaattgc
     3361 gtgcgccctt ccatttttat caataattgt cgttgcatag ggtaacagaa gcactccaag
     3421 gtctctgtac ttttttgttg cttcatcata taacatccag tgctggtttt cacatgtgac
     3481 aacatatatc aaatgccttt gttcgtctgc aacaacatct attactcctt cgccaggata
     3541 tggcattcca agagattctg ccttttgagt tctggggtca tatgccatca catagccacc
     3601 aggatactca gaagtatccc caggactgcg gtatccctgt ttgcttccca cgtaaatttt
     3661 tccactcgag cccacaaaat ttttggtatg aatttttgcc tgcgcagcat aaccagttgc
     3721 attaagtccg catactctgt gtgtgtcgat gacaatcttc tgactttctg tgtacgggtc
     3781 aaattccaca agataggaat tttcattgta ctttgcagta ccaatgtata tctttccatc
     3841 aagaccttcg cagagagaaa aataacctga ttcattgttg tgagtaccag gaagaacatg
     3901 atatgccttt gcatacacat acttaaatgg tgcagtgtga gattgctgct gacctgttgc
     3961 ggcaaataca gaaagtagtg ccaatatccc aaaaagaaag attttctttt tcactgtatc
     4021 tccctgtatc agtcagataa tataattcac aatttctgga ttgtaaaggg acaccgccac
     4081 aaaatttgac atacattttt atatgtgtaa aaacaagtac aaattttcaa aagggggtga
     4141 aaaaaatgaa aagaaagggc tttacactta ttgaacttct tgtagtaata gccattattg
     4201 cagttcttgc tgcgatgttg cttcctgcac tcagta
//
LOCUS       DAORYU010000120         4188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_14234_length_4188_cov_5.099685, whole genome shotgun sequence.
ACCESSION   DAORYU010000120 DAORYU010000000
VERSION     DAORYU010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14234_length_4188_cov_5.099685"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="PLW07_04755"
     CDS             complement(<1..718)
                     /locus_tag="PLW07_04755"
                     /inference="COORDINATES: protein motif:HMM:NF024912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49868.1"
                     /translation="MEIQENCGIYGIYSNQDCIHHLFHGIFKLQHRGQRYCGIATFNK
                     ELKLITHRGFVRHSFTPEELQSLSGNIGIGHVSLSERQPVLLESKLGLFVTAFSGNIM
                     NAEQLFEKMKKKGHSFSSHTQVELIAKIIGQGKNFVDGIEKLAQQVKGSYTIVILTKD
                     GIYATRDKYGFKPLILGKSENEYVVSSESRPFSMFSIKIVRDVKPGEIVFIGKNGIKT
                     VGQVKSNRIAYCAFEWGYIAS"
     gene            complement(834..2570)
                     /locus_tag="PLW07_04760"
     CDS             complement(834..2570)
                     /locus_tag="PLW07_04760"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOL49869.1"
                     /translation="MKSSDFIIPEKEVILKKGELRKQLKNKLNKEARKIIDTDIPVVT
                     YSMFKLFHLTGDRVTYEKPFREKRRNLVILAMAYYLTGNKKYLMKLQDYIWETCNEPS
                     WSLPAHLPETLNYEKRYIDLVVSTTAATLAEILALTGDHLDENIKKRIMYELEKRVFI
                     PFYQHPGDYGWFERYDSNWCAVCCGAIGTAVILAGRNQVYFEKIVNNVAQAISRYFDN
                     FDTQGAWVEGISYWNYGLTHAVRFADTLYRATDGKLNLFNHLKIQLTGLFPVHCFLPP
                     DGFVNFGDCHRHVALTRETMLLLAIYTKAGKYISWLARHVTMSDLQEIRSLRDIKIPA
                     GSVPDETFVHYPDIGWVITRKNWKDKNGPVFAIKAGNNNEPHNQIDVGQFIFHVFGED
                     YLCDHGAGKYTKDYFSAKRYQNPFCSAEGHSVIFIDGKGQGIGPEFCGKILKAQHSKD
                     VDEVVVNLIGAYPVGLASNITRTATFSKKQKYGLLLIDDFIETSEERTIESRLQYNGQ
                     LKKLDRSHFILKGKNGKVAINILKPEKFSVSIGKFGNLEEIAGEKTPIQFLKITTKSR
                     KTRFLIEFQPVL"
     gene            complement(2557..3660)
                     /locus_tag="PLW07_04765"
     CDS             complement(2557..3660)
                     /locus_tag="PLW07_04765"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOL49870.1"
                     /translation="MATMTKRERCERTMNFQETDRIPIYDLLRCDAAFEYFSGEKLPP
                     LSKSPDIEEKLAKIVGKAVNKLLDMTRSVGFGPIVEEDIEDYYGFIRHSSPSEKTTWI
                     KKKPFLDEKGAIEFLKRWVSDIKKDRKAIEHNPSEYREKYHDGFLKTQALIGDTVNLF
                     AQQGVGLDDIRVQMGFELFSYVYADDPGIISECLEECTKRNITICHAIADVKLSPCVL
                     TYGDIACKQRLLHSPEFLRKEFFPRLKRINDAWHEHGFKCLFHSDGYLMDVMDDLIAT
                     GIDGLNPIETVAGMSIKEIREKYGNKIFLAGGIDMSQLLAFGNTEGVKEYCRKTIKEA
                     YPGYFIGSTTEIDNSTKLENIIAIYEVVHNEKF"
     gene            3929..4120
                     /locus_tag="PLW07_04770"
     CDS             3929..4120
                     /locus_tag="PLW07_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49871.1"
                     /translation="MGRFQKIPPTSGLLPLKKEAGMDLMKSLLHLPFVKGENDKIPLC
                     PPFEKGEILRWNFTKGDEE"
BASE COUNT         1163 a          861 c          740 g         1424 t
ORIGIN      
        1 ttgaagcgat gtatccccat tcaaatgcgc agtaagcaat acgattactt ttcacctgtc
       61 ccacagtttt aattccattt ttcccaataa atacaatctc acctggtttt acatccctga
      121 caatttttat actgaacatg ctgaaaggcc ttgattctga agaaacaacg tattcatttt
      181 ctgattttcc aagaatgagt ggcttgaatc cgtatttatc ccttgtagcg taaataccat
      241 cttttgtaag aatcacaatt gtgtaagaac cctttacctg ttgcgcaagt ttttcaatgc
      301 catcaacgaa gtttttgccc tgaccaatga tttttgcaat gagttccacc tgggtatggc
      361 ttgaaaacga gtgtcctttt ttcttcatct tctcaaaaag ttgttctgcg ttcataatat
      421 ttccgctgaa cgcagtaaca aacagaccca gttttgattc aagaagaaca ggttgtcttt
      481 cactcaaact tacatgtccg attccgatat ttcctgaaag ggactgcaat tcttcaggag
      541 taaaagaatg cctgacaaac cccctgtggg tgatgagttt taattctttg ttgaaggtgg
      601 caatgccgca gtacctctgc cctctgtgct gaagtttgaa aatgccgtga aaaagatgat
      661 ggatacaatc ttgatttgag taaattccat agattccgca attttcctgt atttccattt
      721 tacattctcc tgtgttctgg atggctaaca cggatggtat tataccacaa aaaccatata
      781 aaatttccca gacccttgct aatgttttct ctgttttcta tcaatcacag accttataaa
      841 actggctgga attctattaa aaagcgggtt ttgcgtgatt ttgtcgtgat ttttagaaat
      901 tgaatgggtg ttttttcacc agcaatttct tcaaggtttc caaatttgcc tattgacaca
      961 gaaaatttct ctggtttcaa aatattgatg gcaactttcc catttttccc tttcagaata
     1021 aagtgtgacc tatcaagttt tttcagttga ccattgtatt gaaggcggct ttctattgtt
     1081 ctttcttctg aagtttcaat gaaatcgtca atgagaagca agccgtactt ttgttttttt
     1141 gaaaatgtgg ctgttcttgt gatgtttgag gcaaggccga ccgggtaagc gcctattaaa
     1201 ttcacaacaa cttcatcaac atctttgcta tgttgtgcct ttaaaatttt tccacaaaat
     1261 tcaggaccta ttccctgccc tttgccgtca ataaaaatca cactgtgtcc ttcagcgctg
     1321 cagaatggat tctgatatct ttttgcactg aaatagtcct ttgtatattt gccagcacca
     1381 tggtcacaca gataatcttc tccaaagaca tgaaatataa actggcccac atcaatctga
     1441 ttatgtggtt cattgttgtt gcctgccttt atggcaaaaa caggtccgtt tttgtctttc
     1501 cagtttttcc ttgtgatgac ccagccaata tcaggataat ggacaaaggt ttcatcagga
     1561 acagaaccag caggaatctt aatgtctctt aaagacctga tttcctgtaa gtcagacatt
     1621 gttacatgtc tggcaagcca ggaaatgtat tttcctgctt ttgtgtatat ggcaagaagc
     1681 agcatggttt ctcttgttaa agcaacatgc ctgtggcaat ctccaaaatt cacaaatcca
     1741 tcaggtggca aaaagcaatg cacagggaaa agccctgtca gttgtatttt tagatggttg
     1801 aaaagattaa gttttccatc tgttgccctg taaagtgtgt cagcaaatct tactgcatgg
     1861 gtaagaccat aattccagta agagatacct tcaacccatg ctccctgagt atcaaaattg
     1921 tcaaaatatc tgctgatggc ttgcgcaaca ttgtttacga ttttttcaaa atatacctga
     1981 tttcttcctg cgagaattac agcagttcct atggctccac agcacacagc acaccagttt
     2041 gagtcatatc tttcaaacca accatagtca ccagggtgct gatagaaggg aatgaaaacc
     2101 cttttttcaa gttcatacat aattcttttt ttgatgtttt catcaagatg gtctccagta
     2161 agcgccagta tttcggcaag ggttgcagcg gttgttgata caacaaggtc aatgtatctt
     2221 ttttcataat tgagtgtttc aggtaaatgt gctggtaaag accaacttgg ttcattgcat
     2281 gtttcccaga tgtagtcctg aagtttcata agatattttt tgtttcctgt cagataatat
     2341 gccattgcaa gaatgaccag gtttcttctt ttttccctga atggtttttc gtaggttact
     2401 ctgtcgcctg tgagatgaaa taatttgaac atggaataag tgacaactgg aatatcagtg
     2461 tcaatgattt ttctggcttc tttgttgagt ttgtttttca gttgtttcct gagttcacct
     2521 tttttcagta tgacttcttt ttctggaatg ataaaatcag aacttttcat tgtgcaccac
     2581 ttcataaatg gcaatgatgt tttcaagttt tgtgctgtta tcaatttcag tggttgaacc
     2641 aatgaaataa cccggatacg cttcctttat ggttttcctg caatattctt tcaccccttc
     2701 agtatttccg aatgcaagta actggctcat gtcaataccg ccagcaagaa aaattttgtt
     2761 tccatatttt tccctgattt cctttatact cattcctgca actgtttcta tgggattgag
     2821 cccatcaata cctgttgcaa ttaaatcatc catcacatcc atgagatacc catcagaatg
     2881 aaagaggcac ttaaatccgt gttcgtgcca ggcatcattg attcttttca atctgggaaa
     2941 aaattctttt cgcagaaatt caggcgaatg aagaagccgt tgtttgcagg caatgtctcc
     3001 atatgtaagc acgcatggag aaagttttac atcagcaatt gcatgacaga ttgtgatgtt
     3061 tcttttggtg cactcttcaa ggcattcaga tataatacca gggtcatctg catatacata
     3121 tgaaaaaagt tcaaatccca tttgtaccct tatgtcatca agtccgactc cctgctgggc
     3181 aaaaagattc acagtatctc cgatcaatgc ctgtgttttc aaaaagccat catgatattt
     3241 ttccctgtat tcagatgggt tgtgttctat tgcttttctg tcttttttta tatctgaaac
     3301 ccatcttttc aaaaattcaa tggctccttt ttcatctaaa aatggttttt tcttaatcca
     3361 ggttgttttt tctgaaggac ttgaatgacg gataaaccca taataatctt caatatcttc
     3421 ttctacaatg ggtccaaatc caacgcttct tgtcatatca aggagtttat taactgcctt
     3481 tcctacgatt tttgctaatt tttcttcaat gtcagggctt tttgaaagtg gtggaagttt
     3541 ttctccactg aaatattcga atgcagcatc acaacgcaat aaatcataaa tgggaattct
     3601 gtcagtttcc tgaaaattca tcgtcctttc acatcgttct ctttttgtca ttgttgccat
     3661 agtcaacaat tttatcacaa aaaaaattga ttacaatgca cattctaatt ccttctttat
     3721 aaaattacct catactacct cccgctttgt aaaatcacct catactaccc cccctctttg
     3781 taaaatcacc tcaaactacc tcccctcttt gtaaaatcac ctcgtactat cacccctctt
     3841 tataaaagag gggctggggg agatttcttg aaatccccct tagtccccct tttgtaaagg
     3901 gggaaaacag ggggagggga aagaggggtt gggaagattt caaaaaatcc cgccaacttc
     3961 aggtctcctt cctttgaaaa aggaggctgg gatggatttg atgaaatccc tcttgcatct
     4021 cccttttgta aagggagaaa atgataaaat ccccctttgt cccccttttg aaaaggggga
     4081 aattttaagg tggaatttta caaaggggga tgaagagtaa gtaattatta aaaatatagc
     4141 aacagcacat ccactgtttt caatggcaat gtaactttgg cactattt
//
LOCUS       DAORYU010000121         4180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_14264_length_4180_cov_5.146424, whole genome shotgun sequence.
ACCESSION   DAORYU010000121 DAORYU010000000
VERSION     DAORYU010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14264_length_4180_cov_5.146424"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..3065
                     /locus_tag="PLW07_04775"
     CDS             81..3065
                     /locus_tag="PLW07_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49872.1"
                     /translation="MKRIMAGMVCVFLMGSFCGAQVVKVQPWKKDVTLISKYLTVPID
                     ELVNHKGFLNVSENRLIVGNIPFQLSSRSDANCIFMKDVGWKDWQKDPSSYYSAYDAV
                     PTEPDPTRMMFHIPVDYYSAVYVLASCENDKNLSNVLTLRIGKFGGGHGMTKYTDFVA
                     QIPRADSPRGGIGLIKTETGNVFLVKIPLDNAIWQEFKNVSTIDVDITKEIRLAMRRP
                     DPCRYQTRPLGLPSGIHIYGITFERAPMKMELYTDEAGHVFNEPQIPQFNVVLTKMTP
                     IRQCDIEAKAVDYYGNVTAVTISDIKIPNFGSKVIPLKLQVQKRGYYDLSVTLKVNKQ
                     PIFTRYTSFALLPPDDRKYRHESPFGTWDFCGGHFTSDDPDVVGPLYVKAGLRYGMFG
                     FSDEARKKYGVIKGNDMKLDPKGIEALAEKIKTDTSLKPPERLMIFHENAISGEHITR
                     VPDVFTGRKYVLSEAEQKKFKEMWDIAEQTAKAIRKYFPKTEIYFGNGAPQLLEEFCR
                     NKFPAELLGSRGNEAGNFMRMPETQPPDFIANNAGLWMDRMILDHYGYKNTPLRQCYE
                     MCYPNTNPGNLSPQTQAKYLIRHMMHSLAWRIPIIRAGIITDVGNSYYFSNWGASGLC
                     YGLPDIRPKPAYVAIATMTRALDGASFKRAIPTNSSVVYGLEFGKKDGRYVNCLWTIR
                     GTRDIEIVYKNKPRAILTDMMGNEVGLEPEGKSVFVSISSEPVFITVNQIVEKIVAGN
                     AYMEKRPVSSFIISGLNNLNDWIIEKERNRELEIYNFNCPRRKGNFEFKIVDAFEGET
                     NVLQIKPKLPVEGSEYLPMYEVLAHKTGIEIPGVPTKIGLVVNGNGGWGRVIFELEDA
                     SGQRWISIGAEQKGEPTRWMADWFKPEEFAQLKSANLCDWNTDDAWQRSYINFEGWKY
                     LEFPLPGNYPGEGYHWPYSSQWRYTGDGIVKYPLKFKKLIITIPEKILYLTEYKPVQR
                     QEIYIKDLMVTYQPPEMAFKAE"
     gene            3068..>4180
                     /locus_tag="PLW07_04780"
     CDS             3068..>4180
                     /locus_tag="PLW07_04780"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOL49873.1"
                     /translation="MKHAIIFTMLCFCLLLTGSLNAEKVKIGLEVLQEKNFDILRGKK
                     VGIITNQTGVDSNFNSVVDILKNAKDVTVVCVFSPEHGFFGGVQGNVNDITDPKTGVP
                     VFSLYGSTRTIPEDKLKNIDVIVYDIQDIGSRSYTYISTMKLAMEQAAKCNKEFVVLD
                     RPNPINGLIVDGPVLDTKFRSFIGIAEIPYVHGMTTGELALFFNSELGINCNLTVVPM
                     KNWKRDMSWQDTLLPWIPTSPHIPEPDTPYFYPITGVIGELSLVSVGVGYTLPFKIIG
                     APWIDAEQFTTELNSKKIEGVYFHPFYFKPYYVIFKDQFCQGARIVITDKNKVKPFLT
                     AIMIMEVLKTLYPDKFNFSRPDIKNRIPTFDNACGTD"
BASE COUNT         1336 a          663 c          943 g         1238 t
ORIGIN      
        1 gaccattgtt tacttattgc ttgacttgta ttcctgttca atgtaaatta tcaggacacg
       61 atttcttaca gggagacaaa atgaaaagaa ttatggcagg gatggtatgt gtttttctaa
      121 tgggttcttt ttgtggtgcc caggttgtaa aagtccagcc atggaagaaa gatgttacac
      181 ttatttcaaa atatcttact gtaccgattg atgaattagt aaaccacaaa ggatttttga
      241 atgtttctga aaacaggtta attgttggaa atatcccttt tcaattgtca agcagaagtg
      301 atgccaattg catatttatg aaagatgtgg gatggaagga ctggcaaaaa gacccaagtt
      361 catattattc tgcgtatgat gctgtaccca ctgaacctga tccgacaaga atgatgtttc
      421 atattcctgt tgattattat agcgccgtgt atgttcttgc aagttgcgaa aacgataaaa
      481 acctcagtaa tgttcttact ttaagaattg gcaaatttgg tggcggtcat ggtatgacaa
      541 aatatactga ttttgttgcg cagattccgc gcgctgatag tcctcgtggc ggaattgggt
      601 tgattaaaac agaaacagga aatgtatttc ttgttaaaat acctcttgat aatgcaatat
      661 ggcaggagtt caagaatgtc tccacgatag atgttgatat taccaaagaa attcgccttg
      721 caatgagaag acctgatcca tgcaggtatc agacaagacc actgggactg ccgagcggaa
      781 ttcatattta tggcattacg tttgagcgag cgccaatgaa aatggaactt tacactgatg
      841 aagcaggtca tgtttttaat gaaccacaga taccacagtt taacgttgta ttgaccaaaa
      901 tgacacctat caggcaatgt gacattgaag caaaagccgt agattactac gggaatgtga
      961 ctgctgttac aatttctgat atcaagattc caaatttcgg gtcaaaagta ataccattga
     1021 aattacaggt tcaaaaaaga ggttattatg acctttctgt tacgctgaaa gtgaacaaac
     1081 agccgatttt tacaagatat acgagttttg ctttattacc acctgacgat agaaaatacc
     1141 gtcatgaatc tccgttcgga acctgggatt tttgtggagg gcattttaca agcgatgatc
     1201 ctgatgttgt aggacctctc tatgtgaaag ctggtttgag atacggaatg tttgggtttt
     1261 ctgacgaagc aaggaaaaaa tatggtgtta ttaaaggaaa tgatatgaaa ctggatccaa
     1321 aagggataga ggcgctggct gagaaaataa aaactgatac atccttaaaa ccacccgaga
     1381 gattgatgat tttccatgaa aatgcaatat ctggtgagca tatcacgaga gttccagatg
     1441 tttttactgg aaggaagtac gtcttgagcg aggcagaaca gaagaaattc aaggaaatgt
     1501 gggatattgc agaacaaaca gcaaaggcaa tcaggaaata ttttccgaag acagaaattt
     1561 attttggaaa tggcgctccg cagttgcttg aagagttttg tagaaataaa tttcctgctg
     1621 aacttctggg gtcaaggggc aatgaagcag gtaattttat gaggatgcca gagacacagc
     1681 cgcctgattt tattgccaac aatgctggat tgtggatgga tagaatgatt cttgatcatt
     1741 atggctacaa aaatactcca ttgaggcaat gctatgaaat gtgctatcca aatacaaatc
     1801 ctggtaatct ttcaccacaa acccaggcaa aatatctaat caggcacatg atgcattcac
     1861 ttgcatggcg tattccgata atcagagccg gaattattac agatgttggc aattcttatt
     1921 atttcagcaa ttggggggca tccgggcttt gttatggact tcctgacatc agaccaaaac
     1981 ctgcctatgt tgctattgcg accatgacac gggctcttga tggtgcatct tttaagcggg
     2041 caatcccgac gaattccagt gttgtatatg gacttgagtt tggtaaaaaa gacggcaggt
     2101 atgtcaattg tctgtggact atcagaggaa caagagatat tgaaattgtg tataaaaaca
     2161 aacccagggc gattttgact gacatgatgg gtaacgaagt ggggcttgaa ccagaaggaa
     2221 aatctgtttt tgtgagtatc tcatcagaac ctgtttttat tactgttaat cagatagttg
     2281 agaaaattgt tgcaggcaac gcgtatatgg agaaaagacc ggtatcttct tttattatct
     2341 ctggtttgaa taacctcaat gactggatta ttgaaaagga aagaaacagg gaacttgaga
     2401 tttataactt taactgtcca agaagaaagg gcaattttga attcaaaatt gtggatgctt
     2461 ttgagggtga aaccaatgta ttacaaataa agccaaaatt gccggttgag gggtcagaat
     2521 atctaccgat gtatgaagtt cttgctcaca aaacgggtat tgaaataccc ggagttccca
     2581 caaaaattgg tttggtggtc aatggaaatg gaggatgggg aagagtgatt tttgaacttg
     2641 aggatgcgtc tggtcagagg tggatatcta ttggagcaga gcagaaaggc gaaccaacga
     2701 gatggatggc tgactggttc aagccagagg aatttgcgca attgaaaagt gcaaatcttt
     2761 gcgattggaa tacagacgat gcctggcaga gaagttatat caattttgaa gggtggaaat
     2821 atcttgaatt tccactgcct ggtaattacc ctggagaagg ttatcactgg ccatatagca
     2881 gtcaatggag atatactggg gatggtattg tgaaatatcc tttgaaattt aaaaaactta
     2941 ttatcacgat tccagaaaag attctatacc tgacagaata taagccagtg cagagacagg
     3001 aaatttatat caaggatttg atggtgacat atcaaccgcc agaaatggct ttcaaagcag
     3061 agtgactatg aagcacgcaa ttatttttac gatgctgtgt ttttgtttgt tgttgaccgg
     3121 ttcattgaac gcagaaaaag tgaagatagg attggaagtt ttacaggaaa aaaactttga
     3181 tatattgaga ggtaaaaaag tgggtattat tacaaatcaa acaggtgttg attctaattt
     3241 taattctgtt gtagacatat tgaagaatgc aaaagatgtg acagttgttt gtgtattttc
     3301 tcctgaacac ggattcttcg gcggtgttca gggaaatgtt aatgatatta ctgacccgaa
     3361 gaccggtgta cctgtgttca gtttatatgg ctcaacgaga acaattcctg aagataaatt
     3421 aaaaaatatt gatgtgattg tgtatgacat tcaggatatt ggttcacggt cttataccta
     3481 tatcagcaca atgaaattgg cgatggagca ggcagcaaaa tgtaataagg aatttgttgt
     3541 gctggataga ccaaatccga taaacggact cattgtggat ggacctgtgc ttgacacaaa
     3601 attcaggtct tttatcggaa tagcagaaat tccttacgtt catggaatga caacaggaga
     3661 actggcatta ttttttaatt cagaacttgg aattaactgt aatttgacag tggttcctat
     3721 gaaaaactgg aaaagggata tgtcatggca ggatactttg ctcccctgga ttccaacttc
     3781 tccacatatt ccagaaccag atactccata cttttatccc attactggtg taattggaga
     3841 attatccctg gtaagtgtgg gggtaggata tacgcttcct ttcaagatta ttggtgcgcc
     3901 ctggattgat gcagaacaat ttacaactga attaaacagt aaaaagatag aaggtgtcta
     3961 ttttcatcca ttttatttta aaccatatta tgttatattt aaggaccagt tctgtcaggg
     4021 tgcaaggatt gttatcactg ataagaacaa agtgaaacct tttttgactg caattatgat
     4081 tatggaagta ttgaaaacgc tgtatcctga taaatttaat ttttctagac cggatataaa
     4141 aaatcgtatt cctacatttg ataatgcctg tggtacagat
//
LOCUS       DAORYU010000122         4161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_14356_length_4161_cov_3.504627, whole genome shotgun sequence.
ACCESSION   DAORYU010000122 DAORYU010000000
VERSION     DAORYU010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14356_length_4161_cov_3.504627"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="PLW07_04785"
     CDS             complement(<1..1136)
                     /locus_tag="PLW07_04785"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOL49874.1"
                     /translation="MISKKNFIFLSIFAIFIWVRSSCCEGVSTAPYSEIEFAPGENIV
                     VFAPHPDDEIIGCGGIIQEYLNKDCTVHIVYLTNGDHNQFAFKAYEKRLILSPSSYLK
                     MGEVRRKESTKATQICGIPGKNLIFLGYPDFGTLHIWRDYWNVKKPFTNFLTRKNYVP
                     YADGFSFGNAYTPENIQSDIVKILKNIKPSKIFVSHPADLNPDHRALFNFVRLVLLDC
                     KDLFEPKVYCYLVHARNWPQPEGLFPDKLLKPPDVLEKSGSWSIFYLTEQQKSNKILA
                     LDCFKSQLIGRKNWAFSFVRDNEIFHNIPTINFKRNNGTEISFDAHAQEAVSDEESLK
                     HSKVYNLKLTYDQDNIVFNLSFDRKVLEKEFGVKLYVYGWKQKVP"
     gene            1160..1399
                     /locus_tag="PLW07_04790"
     CDS             1160..1399
                     /locus_tag="PLW07_04790"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl domain-containing protein"
                     /protein_id="HOL49875.1"
                     /translation="MEKQKIIAKFSEEIEKASVSFWYVGEGDSIEKEQPLIEFVTEKT
                     SFTYASPVSGKIIRTFVNEGDEVISGQDIAEVEIQ"
     gene            1396..2571
                     /locus_tag="PLW07_04795"
     CDS             1396..2571
                     /locus_tag="PLW07_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006890210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOL49876.1"
                     /translation="MKEIESSRIKNLPGYLFQTINERKLKARQEGRDVIDLGMGNPDM
                     PPPEPVVEKLKETVTNPKVHRYSASKGIPHLRKEICLWYERRFGVKLNPDEEAIATIG
                     SKEGISHLALAIFDRGDVVLTPNPTYPSHFYSVIIAGATVYDVPLLPENNFLPDLNQP
                     IYDRFPVPKAMIVSYPNNPTTQVVDLDFFKELVRFAKRNNIIIIHDLAYSEICFDGYR
                     APSFLQVEGARDVGVEFYSLSKTYNMAGWRVGFMVGNKFIVRALAKLKSYYDYGIFTP
                     VQVASICALRLEEKYINKTVEEYRKRRDTLVDGLNRIGWTVEKPVATMYVWAKIPEQF
                     RHMNSLDFALYLLDECNLVVSPGLGFGKYGEGYVRIALVENEHRIRQAIRSLKKIFK"
     gene            complement(2791..2864)
                     /locus_tag="PLW07_04800"
     tRNA            complement(2791..2864)
                     /locus_tag="PLW07_04800"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2828..2830),aa:Val,seq:gac)
     gene            2941..3306
                     /locus_tag="PLW07_04805"
     CDS             2941..3306
                     /locus_tag="PLW07_04805"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOL49877.1"
                     /translation="MPKLKFNADFMGPEYSFEKKDIFIGRSPENDVTIPDYRIFAGLP
                     ITVQNARYRTLRHVSRRHAVIQFIDGKYYVMDIGNDGKGSSHGTFINNERITPGIKCE
                     LKNGDKIRFAEVESLFSLE"
     gene            3403..>4161
                     /locus_tag="PLW07_04810"
     CDS             3403..>4161
                     /locus_tag="PLW07_04810"
                     /inference="COORDINATES: protein motif:HMM:NF014862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HOL49878.1"
                     /translation="MNWEKFTTKSQETLMRAQAIAQQKRQQQIDVVHLALAMVESDFI
                     QDLFKQMQVNNSEVKNKLGKELEKLPEVSGTPGQVYISGLLNTVLIQAEQEARNLGDE
                     YTSLEHILLALCEIDSNVKEIFKKTGIFKDRVMKFVEQLRGGEQASDASAEDRYQALK
                     KYTRDLTDLAAKGKIDPVIGRDEEIRRVTQVLCRRTKNNPVLIGEPGVGKTAIVEGLA
                     RRIDSGDVPESLKNKRILMLDLGALVAGTKFRGEF"
BASE COUNT         1375 a          750 c          840 g         1196 t
ORIGIN      
        1 ggaacttttt gcttccagcc ataaacgtat aatttaacgc caaattcttt ttcaagaact
       61 tttctgtcaa aggacaaatt gaatacaata ttatcctggt cgtaagtcag tttcagattg
      121 taaaccttgc tatgcttaag actttcctca tcagatactg cttcctgagc atgtgcatca
      181 aaagagattt cagtaccatt attccttttg aagttaatcg tgggtatgtt gtgaaatatc
      241 tcgttatctc ttacaaagga gaacgcccag tttttacgtc ctattaactg ggatttgaaa
      301 caatcaagag caagtatctt gtttgatttc tgttgttcgg ttaaataaaa aatactccag
      361 gatcctgatt tttccaaaac gtctggcggt tttaataatt tatcgggaaa caaaccctct
      421 ggttgtggcc aatttctagc atgtacaaga taacagtaaa ctttgggctc aaacaaatct
      481 ttgcaatcaa gcaatacaag acgaacaaaa ttaaacagtg ctctatggtc aggattcagg
      541 tctgctggat gagatacaaa gatcttggaa ggttttatgt tcttaagtat ttttacgata
      601 tctgactgta tgttctcagg agtatatgcg tttccaaaag aaaaaccatc ggcatatgga
      661 acataattct tgcgggttaa gaaatttgtg aaaggtttct tgacattcca gtaatccctc
      721 catatatgaa gtgtgccaaa atcaggataa ccaagaaaaa tgaggttctt tccagggata
      781 ccacaaattt gtgttgcttt cgtggattct tttctgcgta cctcgcccat ttttaagtaa
      841 ctggaagggc ttaaaatcaa ccttttttca tatgccttga aagcaaactg attatgatct
      901 ccgtttgtca aataaacaat gtgtacagtg caatctttgt taaggtattc ctgaataatt
      961 ccaccacagc cgatgatttc gtcgtcagga tgtggggcaa acacaacaat attttcacct
     1021 ggcgcaaatt caatttcgga atatggtgca gtggatacgc cttcacaaca ggaacttcgt
     1081 acccatataa aaatggcaaa aatactcaaa aaaataaaat tctttttact aatcatggaa
     1141 aatattatac aataattata tggaaaagca aaaaatcatt gcaaaatttt cagaagagat
     1201 tgagaaagcc tctgtgtcat tctggtatgt aggagaaggc gatagcatag aaaaggaaca
     1261 gcccctcatt gagtttgtga cagagaaaac ttcctttact tacgcaagcc cagtttcagg
     1321 aaagattatc agaacatttg tcaatgaagg agatgaagtt atctccggtc aagatattgc
     1381 ggaggttgaa atacaatgaa agaaatcgaa tcatcccgaa tcaaaaatct gccgggatat
     1441 ttattccaga caatcaatga aagaaaattg aaagcgcgac aggaaggcag agatgttatt
     1501 gaccttggca tgggaaatcc tgatatgccg cctccagaac ctgtggtcga aaaattgaag
     1561 gaaactgtta ctaatccgaa agtccatcgt tatagtgcct caaaagggat tcctcacctt
     1621 cgaaaagaaa tttgcctctg gtacgaaaga agatttggcg taaaacttaa ccctgacgaa
     1681 gaagctattg caactattgg ctcaaaagaa ggaatcagtc atctggcact tgccatattt
     1741 gacaggggtg atgtagtgct aacaccaaat ccaacttatc ccagtcattt ctatagtgtg
     1801 attatagcag gcgcgactgt atatgatgtt cctttattac cagaaaataa ttttttgcct
     1861 gatttaaatc aaccaatcta tgatagattt cctgttccaa aagcaatgat tgttagttat
     1921 ccgaataatc ccaccaccca ggttgttgac ttagattttt tcaaggaact tgtcagattt
     1981 gcaaaaagga ataatatcat tattattcat gacctggcat attctgaaat atgttttgat
     2041 ggatacaggg ctccgagttt cttgcaggtt gaaggcgcca gggatgttgg agttgagttt
     2101 tattcccttt caaaaacata caacatggct ggctggcggg ttggattcat ggttggcaac
     2161 aaattcattg ttagagccct cgcaaaactg aaaagttact atgattacgg aattttcacc
     2221 cctgttcagg ttgcctcaat atgtgcattg aggttagagg aaaaatacat caataaaact
     2281 gttgaggaat atagaaagag acgcgatact cttgtagatg gtctaaatag aatcggatgg
     2341 acagttgaaa agccagtcgc cacgatgtat gtctgggcta aaataccaga acagttcagg
     2401 catatgaatt ctcttgattt tgccctttat cttcttgacg aatgtaatct tgtggtatca
     2461 ccaggacttg gatttggaaa atacggagaa ggatatgtca gaattgcact ggttgaaaac
     2521 gaacacagga taagacaggc aatcagatct ttaaaaaaaa tttttaaata aatttctatc
     2581 ttgaacaagg cttttcttca tcccttagaa tcaaacgttc aaatcaatta aatcgcctgc
     2641 tatacgatgc tcattgttct tcgcatcagc agggacttgg cgccctcgtc tcccactacg
     2701 actcgggtcg gccagccgct ggaattgctg cgctcgctcg caattcctgc ttcgctccat
     2761 tcaagtcctg cactaattaa aaaaaaaaaa tgggcggtgc aggacttgaa cctgcggcct
     2821 ttacgatgtc aacgtaacgc tctggccacc tgagctaacc gccctgacaa atattataac
     2881 agaaaacttg aaaaatcaaa aaaaatatgg gaaaatttta gtaaggaatg ggggtttatt
     2941 atgccaaaac tcaaatttaa tgctgatttt atgggacctg aatattcgtt tgaaaagaaa
     3001 gatattttta ttggaaggtc tcctgaaaat gatgttacaa tacctgatta ccggattttt
     3061 gcgggattac caataactgt tcaaaacgca agatacagaa ctttgcgcca tgtctcaaga
     3121 agacacgctg ttattcaatt tatagacgga aaatactacg ttatggatat tggtaatgat
     3181 ggaaaaggaa gcagtcatgg aacttttatt aataatgagc gtattacccc tggcatcaaa
     3241 tgtgaactga aaaatggcga caaaatcaga ttcgctgaag ttgaatcctt gttttctctt
     3301 gaataatacc ctttttcaat aatcaaccga ttacgcatcc tttttttgat ttaactcatc
     3361 caaatattag caggcaaaaa tttaagcata aggagaaaca ttatgaactg ggaaaaattt
     3421 acaacaaaaa gccaggaaac attgatgagg gctcaggcaa tagcccagca aaaacgccaa
     3481 caacagattg atgttgtgca tttagccctt gcaatggttg agtcagattt tattcaggat
     3541 ttattcaaac agatgcaggt gaataactct gaagtgaaaa acaaacttgg caaggaatta
     3601 gaaaaattac cagaagtatc aggtacgcca ggtcaggttt atatatcagg ccttctcaat
     3661 actgtactca ttcaggcaga acaggaagcc agaaatcttg gtgatgaata tacaagcctt
     3721 gaacacattc ttctggcgct ttgtgaaatc gatagtaacg tgaaagaaat ttttaaaaaa
     3781 actggaattt tcaaagatag ggttatgaaa tttgttgagc aacttcgtgg aggagaacaa
     3841 gcaagtgatg ccagcgcaga agataggtat caagcattaa aaaagtacac ccgagacctt
     3901 actgatcttg cagccaaagg aaaaatcgac cctgtgattg gaagggatga ggaaataagg
     3961 cgtgtaacgc aggttttatg cagaagaaca aaaaataatc ctgttttgat cggtgaacct
     4021 ggagttggta aaaccgctat tgttgaaggt cttgcaagaa gaattgattc aggcgatgtg
     4081 ccggaaagtt tgaaaaataa aagaattttg atgcttgacc tcggagccct tgttgcagga
     4141 accaaattca gaggtgaatt t
//
LOCUS       DAORYU010000123         4148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_14402_length_4148_cov_4.423161, whole genome shotgun sequence.
ACCESSION   DAORYU010000123 DAORYU010000000
VERSION     DAORYU010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14402_length_4148_cov_4.423161"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="PLW07_04815"
     CDS             complement(<1..980)
                     /locus_tag="PLW07_04815"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HOL49879.1"
                     /translation="MKSLLLQGDTIKGLILLGGFGTRLRPFTLTTPKSLLPVACVPLI
                     YYQFELLSKHGINSVVLAVNLHSRRRRELFSMAKSLGIKLYLSCEKKPMGTAGAIKNA
                     KKFLQDGPFFVFNGDILTDCNLTEMLHFHKNCGADVSIAGVKVENPNHFGVMIFDHNN
                     RIHNFIEKPQQPVSNIINAGIYIIQPDVLDEIPAGRETSIEKETFPQLIKKGRHVSVY
                     LHSGYWMDVGTIENYRKANFDVVDGKMKIEYKKDHCTVSSAKNVQCEGKLKAADSVMF
                     GENVIIRGTVIIGDNCYIGNGSVLQDVILFKDVFIRQNCVIENSIVGNSV"
     gene            complement(1052..1792)
                     /locus_tag="PLW07_04820"
     CDS             complement(1052..1792)
                     /locus_tag="PLW07_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018557765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOL49880.1"
                     /translation="MFDYNLSIWGWCLLSLAGIFIGVSKTGIPGLGMLSVAMAALVIP
                     ARLSTGVILPMLILGDVFAVAYYRRHARWRYLIKLIPYAAAGIILGYLALGKMTDQQL
                     RPMIGFIVLLLLWLNEADILNRFNIPERWWFAAAIGLSAGFTTMIANAAGSIMIIYLL
                     AMRLPKQEFIGTAAWYFFLLNWFKVPFSASLGLINPDSLLLNLKVSPAIIVGAISGIY
                     LVKIIPQKMFENIIKILTVIAAAKLIIG"
     gene            1910..>4148
                     /locus_tag="PLW07_04825"
     CDS             1910..>4148
                     /locus_tag="PLW07_04825"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49881.1"
                     /translation="MNNKSFSEIEKEIDSVYQSVRQYLWLRTWEFQQVNEPGNTARFD
                     ESAWKTIQDPSWATAEGTAFLRYKIVVPSEVEGIPCGGSDIKIEFIFPSGITLFVDDK
                     EIYSQQYWADMRPDPLKIIENAVPGTTHTIMFKAPKGDGHGGLYAVLYIDALEETLFE
                     LMSIRYQLGFAIAISESRRDKKLKNAVESALGCINISDIRERKWKVVLEQIKQAETIL
                     EPFRPIAKKFTVHLIGHSHIDMNWLWDYENTKDVCIRDFASVVSLMEQFPELTFSQSQ
                     SHVYQIVEQTNPQLFSLALQKIKEKRWEVTANAWVENDLNLVHGESVVRHIIYAKKYA
                     QEKLGNLSPVMWCPDTFGHPATMPSICADAGIKYYFHMRCGKDYPLYRWKGPDGKELL
                     SYKAVYNNWISPDRIVPVLMKFIGLVPKVPHMMFPYGVGDHGGGPTKRDYRMKVRMHA
                     KPVMPSLVFSTVERYFKAVEKYKAKLPVVKGELNTIFEGCYTTHSDIKDINRKCEDIL
                     LVLESAMAVFLSQGGRIPDQDMKKLEELWQKTLFNQFHDILCGSAIKSSYTYSVALGN
                     SAIADARNMIEKYSKQIPEKGGKSLMIFNPCPWERAALVKVDSEKGYYLVEKVPGSGY
                     ITTNLNNMKQSSKRSVKQVSQYEWETDFYHITIDSSTGTIKSLYDKINKKPVLSVAKS
                     AIGEDPSSWWAETSSNLLSVHYEQPHRMSAWIIGNIMSTENLYNINASEVISEPFRII
                     FSVKRK"
BASE COUNT         1347 a          847 c          798 g         1156 t
ORIGIN      
        1 atcacagaat ttccgacaat gctattttca atcacacaat tttgcctgat aaaaacatcc
       61 ttaaaaagaa tcacgtcctg aagcactgag ccatttccga tataacaatt gtctccgata
      121 atgactgttc ctctgataat gacattttca ccaaacatca cactgtctgc ggctttcaac
      181 tttccttcac attgaacatt tttcgctgaa gacactgtac aatggtcttt tttgtactca
      241 attttcattt tgccatccac tacatcaaag ttcgccttcc tgtaattttc aattgttccg
      301 acatccatcc agtacccaga atgaagatac actgaaacat gccttccttt ttttattaac
      361 tgaggaaatg tctctttttc tatggaagtt tccctgcctg ctggaatctc atcaagaaca
      421 tcaggttgaa tgatatatat gccagcattg ataatgtttg aaactggttg ctgtggtttt
      481 tcaataaaat tgtgtatcct gttattatgg tcaaaaatca tcactccaaa atgattgggg
      541 ttttcaacct taacccctgc aatgcttaca tcagcgccac aatttttgtg aaaatgaagc
      601 atttccgtga ggttgcaatc agtaagaata tctccattga atacaaaaaa tggtccatcc
      661 tgcagaaatt tctttgcgtt ttttattgcg cctgctgtgc ccatcggttt cttctcacag
      721 gacaggtaca gttttattcc aagagatttt gccattgaaa aaagttccct gcgtcttctg
      781 gaatgtaaat taacagcaag caccacactg ttaatgccat gttttgaaag cagttcaaac
      841 tgataataaa tcaaaggtac acaggcaaca ggaagaagag attttggagt tgtcaatgta
      901 aatggtctta atctcgttcc aaatccaccc agcagtatca aacctttaat ggtatctccc
      961 tgtaatagta aacttttcaa gtttaattat acctgctatt tttccccatg caaattaagg
     1021 tgaaagtgta aatgatggat attttttcat cctaaccaat aataagtttt gctgctgcaa
     1081 tgactgtaag aattttaatg atgttctcaa acattttttg tggaataatc ttcaccagat
     1141 aaatcccgga aatagcgcca acgataattg caggtgatac ctttagattc agaagcaaag
     1201 aatcagggtt aataagcccc agacttgcac tgaaaggcac cttgaaccag tttaatagaa
     1261 aaaaatacca ggcagctgta ccaatgaatt cctgctttgg aagccgcatg gcaaggaggt
     1321 aaataatcat aatggaacct gctgcgtttg ctatcattgt cgtaaaaccc gcgctcaatc
     1381 caatcgcagc agcgaaccac catctctcag gaatattaaa tctgttgaga atatctgcct
     1441 cattcaacca taaaagtaat agaacaataa accctatcat gggtcgcaat tgctggtcag
     1501 tcattttacc aagagcaaga tagcccagaa tgattccagc cgctgcatat ggtatgagtt
     1561 ttatcaaata cctccacctt gcgtgtcttc tgtaatatgc cactgcaaaa acatctccga
     1621 gaatgagcat cggaagaatc acacctgttg ataatcttgc aggaatcaca agcgcagcca
     1681 ttgctacaga tagcattcca agaccaggaa tacctgtttt tgatacgccg ataaaaatcc
     1741 ctgccagcga aagaagacac cagccccata ttgatagatt gtagtcaaac atcctgctcc
     1801 tttttgaagt tgtcagtatt atacgacatt taaaaaaaat ataaattttc gtctgctgac
     1861 actttttgat aaaataagga aaacttcgtc taaacttcgg gaggaatata tgaacaataa
     1921 aagtttttct gaaattgaaa aagaaataga cagcgtttac cagtcagtcc gtcagtatct
     1981 ctggctaaga acatgggaat ttcagcaggt aaatgaacca ggaaacacag ccaggtttga
     2041 tgagtcagcg tggaaaacaa tacaggatcc gagctgggca actgctgagg gaactgcctt
     2101 cttacggtac aaaattgttg ttccgtctga agttgaaggc ataccctgtg gcggctctga
     2161 catcaaaatt gaattcatat ttccttctgg aatcacactc tttgttgatg ataaagaaat
     2221 ttactctcag caatactggg cagacatgcg gcctgacccc ttgaaaatta tcgaaaacgc
     2281 agtgccaggc actacccata cgattatgtt caaagcacca aaaggagatg gacatggagg
     2341 cctctatgcg gtgttgtaca tagatgctct tgaagaaaca cttttcgaac tgatgtccat
     2401 cagatatcaa cttggttttg ccattgccat ttcagaatca agacgggata agaaactgaa
     2461 aaatgctgtt gaatctgctc tgggctgcat taacatctca gacattagag aaagaaaatg
     2521 gaaagttgtt ctggagcaaa taaaacaggc agaaacaatt cttgaacctt tcaggcctat
     2581 cgcaaaaaaa tttacagtcc atctcatcgg gcattcacat atagacatga actggttatg
     2641 ggattatgaa aacacaaagg atgtttgtat cagggacttt gcgtcggttg tatcactgat
     2701 ggaacagttt cctgaactga ccttttctca aagccaatca catgtttatc aaatcgtgga
     2761 gcagacaaat ccccaattat tttcacttgc gctgcagaag ataaaagaaa aacgatggga
     2821 agtaacagca aatgcctggg ttgaaaacga tttaaacctt gtccacggag aaagcgttgt
     2881 ccgtcacatt atttacgcaa aaaaatatgc acaggaaaaa cttggcaatc tctcgcctgt
     2941 catgtggtgt ccagatactt tcggacatcc tgctacaatg ccctcaattt gtgctgatgc
     3001 aggaattaag tattactttc acatgagatg cggcaaggac tatccactat acagatggaa
     3061 gggtcctgat ggaaaagaac tattgtctta caaagccgtc tacaataact ggatttcgcc
     3121 tgacaggatt gtgccggtgt tgatgaagtt tatcggtctg gtgccaaaag taccgcacat
     3181 gatgtttccc tatggagttg gagaccatgg tggtggtcca acaaaacgag attacagaat
     3241 gaaagtgaga atgcatgcaa aacccgtcat gccgtcattg gtgttttcca cagtggaacg
     3301 atatttcaaa gcagtggaaa aatataaagc aaaactacct gttgtaaagg gagaattgaa
     3361 tacaattttt gagggttgct acacaacgca ttctgacata aaagatatca acagaaaatg
     3421 tgaagatatt ttactggttc ttgaaagcgc aatggctgtg tttctgtcac agggcggtag
     3481 aattccagac caggatatga aaaaacttga agaactctgg cagaaaactt tgttcaacca
     3541 gtttcacgat atcctgtgcg gttcagcaat caaatcatcg tatacatact cagtagcact
     3601 tggtaatagt gccattgctg acgccagaaa tatgatagaa aagtatagca agcagatacc
     3661 agaaaaaggt ggaaaatccc tgatgatatt caatccctgc ccatgggaaa gggctgcgct
     3721 ggtaaaagtt gactcagaaa aaggatacta ccttgttgaa aaagtgcctg gctctggcta
     3781 tattacaaca aatctcaata atatgaagca atcttcaaaa aggtctgtaa aacaagtttc
     3841 tcagtacgaa tgggaaacag atttttacca cattaccatt gacagcagca ctggtacaat
     3901 aaaatctctc tatgacaaga taaataaaaa acctgtcctt tctgttgcaa aaagcgccat
     3961 tggagaagac ccatcgtcct ggtgggcaga aacttccagt aatctgcttt ctgtccatta
     4021 cgagcaaccc catcgtatga gcgcctggat tattggaaac attatgagca cagaaaacct
     4081 ttataacata aacgcaagcg aagtaatcag tgaaccattc aggataattt tttctgtgaa
     4141 aagaaaat
//
LOCUS       DAORYU010000124         4137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_14451_length_4137_cov_3.436306, whole genome shotgun sequence.
ACCESSION   DAORYU010000124 DAORYU010000000
VERSION     DAORYU010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14451_length_4137_cov_3.436306"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="PLW07_04830"
     CDS             complement(<1..365)
                     /locus_tag="PLW07_04830"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HOL49882.1"
                     /translation="MKRILMTLVTVLCLFAGFSFATEGGGGAYPNGAEDFMAGAVPPP
                     GFYYIHYSVGYFSDTFRDSHGKSSVPDFKLTVFGNVSRLIYVSDKKILGGNWGMHILV
                     PLMNVDVKTPAGKDSKFGLG"
     gene            complement(362..991)
                     /gene="thiE"
                     /locus_tag="PLW07_04835"
     CDS             complement(362..991)
                     /gene="thiE"
                     /locus_tag="PLW07_04835"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOL49883.1"
                     /translation="MKYQIEGYYFITDANLTRNGIIKDIKSAIRAGVKIFQYREKNKS
                     ALFMYQEAMEIRTLIKDGIFVINDRVDIALAVNADGVHIGQDDLPLSITRRLLGDKKI
                     IGVTVHNCEQAIRAEKEGADYVAVSPIFKTMTKPDAQEPVGLKILAEVKDAVSIPVVA
                     IGGINLENVPMVVKAGADAICAISSVITKENPEVEIEKFQNIFNNMEEK"
     gene            complement(988..1791)
                     /gene="thiM"
                     /locus_tag="PLW07_04840"
     CDS             complement(988..1791)
                     /gene="thiM"
                     /locus_tag="PLW07_04840"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HOL49884.1"
                     /translation="MENFNIPEILDRVREIKPVVHHLTNWVTIYDCANIVKTLGGSPI
                     MAHAPEEIDNIVSIASSVVLNIGTLTVDFVESMKLAARAANTRKIPVVLDVCGAGATD
                     LRDKKVFELIEYTHIDIIKGNASEIANVAGIKVFTKGVDASDITFDLVEVAESLAQRK
                     SCVVVITGKHDIVSNGNTTFFIKNGHELLTHVVGTGCMVTSVIGTFAAVEKNLVVAAT
                     GGLVCFGIASEIAAEKSKGPGSFKTALFDVLYYLDKETIEDKMNMEKIK"
     gene            1973..3076
                     /locus_tag="PLW07_04845"
     CDS             1973..3076
                     /locus_tag="PLW07_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49885.1"
                     /translation="MVLTYKPDFEQTKIRWRHFWNKEVYKRPIILAGCYKEGINKQFP
                     DVFSLRYYRGYNELWQEQIDLFNEWAECFDFVGDGIPSFSPDFGPDQFSAFYGAKLRF
                     SEHSKHTSLTEPIIGNWNKFLPLKFDPSNETFQKLVAYVRFIAKDAKGKYLVSDIDRH
                     SNIDTLLALRGAEKLCMDLYDNPELIEQAMLSLRKEFPLISDMLYKAGIDNDGLGTTV
                     SWTGGFYSEKNFGVVQADFICMMSPEMFRKFVLPALEEEIEYYDYSYFHLDGPGALKH
                     LDDILSIKKLSVLQWQPGDGQKPNFQWLEILKKAQKAGKAVHVFGNSMQPLTAEIVKK
                     LHKELDPTLVVYDGIPIKDRKEFENLSSWLEKN"
     gene            3131..>4137
                     /locus_tag="PLW07_04850"
     CDS             3131..>4137
                     /locus_tag="PLW07_04850"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOL49886.1"
                     /translation="MEANLHLPWSKEISPPVRSSITFYSKMNDDMIFRKCKTISIYCR
                     IGYCSISLEWTLSRNLFITPFLSGTGRALPGNVFIIDIPAEKLRPGFYDLKVFIDVGE
                     DRELEGLCTFGFEIEKIKYKSILPSDFTKFWESAKNKINTIPLEPEEHFFKKMSEKEI
                     EQYNVKSACLPPKYDGRVRHNKIEVYKISFSSINGLRVYGWLAEPVGKGPFPGMLVLP
                     GAGYRSEPIPAEHARHGYVALDIQVHNQDVNQDKYTKPEWENKPVDYSNPETSEFYTM
                     YLNCVQAVNYLSSRKDVAKDKIVSVGGSQGGRLSVIVAAMDTRIRAIVPAILWGANFL
                     Y"
BASE COUNT         1314 a          823 c          740 g         1260 t
ORIGIN      
        1 cctaaaccga atttagaatc tttcccagca ggagttttca catcaacatt cattaatgga
       61 acaagtatgt gcattcccca gttaccgcca agaattttct tatcactcac atagatcaac
      121 cttgatacat taccaaaaac tgtaagtttg aaatcaggaa cagagctttt accatgagaa
      181 tccctgaatg tatcagaaaa atacccaact gagtagtgaa tgtagtaaaa tcctggtggt
      241 ggaacagcac cagccataaa atcctctgcc ccattgggat atgcgcctcc tccaccttct
      301 gtggcaaaac tgaaacctgc aaaaagacat aacacagtaa ccagcgtcat tagaattctt
      361 ttcatttttc ctccatgttg ttgaatatat tttgaaattt ttcaatttcc acttctggat
      421 tttcttttgt aattacagat gaaatcgcac atatggcgtc tgcgcctgct tttaccacca
      481 taggaacatt ttcaaggttt attcctccta tcgcgacaac aggaattgac acagcatctt
      541 taacctcagc cagaatcttc aaaccaaccg gttcctgagc atccggcttt gtcatcgttt
      601 taaaaatagg cgagaccgct acataatcag cgccttcttt ttcagccctg atggcttgct
      661 cacaattatg aaccgtcaca ccgataattt ttttgtctcc cagtaacctc ctcgtaatgg
      721 aaaggggtag gtcatcctga cctatatgaa ctccgtcggc gttaactgca agcgctatgt
      781 ctactctatc attaatcaca aaaataccat ctttaatcaa tgttcttatt tccattgctt
      841 cctgatacat aaacaaggct gatttatttt tttctctgta ctgaaaaatt ttaacgcctg
      901 ccctgatagc agattttata tctttaatta ttccattgcg ggtgagattt gcgtcagtaa
      961 tgaagtaata tccttctatc tggtatttca ttttatcttt tccatattca ttttgtcttc
     1021 tattgtttct ttatcaagat aatacagaac atcaaataat gcagtcttga aagaacctgg
     1081 gcctttcgac ttttctgctg caatttcaga agcaatgcca aaacagacaa gaccaccagt
     1141 tgcggcaaca actaaatttt tttcaactgc cgcgaaggtt ccaattacag aagttaccat
     1201 gcatcctgtt ccaacgacat gtgttagcaa ttcatgacca tttttgataa aaaacgtggt
     1261 gtttccgttt gaaactatgt catgtttacc agtaataact accacgcatg acttcctttg
     1321 cgcaagtgat tcagcgactt ccaccaaatc aaatgtgata tctgatgcat caaccccctt
     1381 tgtaaaaacc tttattcctg caacatttgc aatttcagag gcatttccct tgatgatgtc
     1441 aatatgtgta tattctatta attcaaacac tttcttatct cgtaaatcag tagcccctgc
     1501 cccacataca tcaaggacaa caggaatttt tctggtattt gcagcccttg cagccagttt
     1561 cattgattca acaaaatcta cggtaagagt cccaatattc aataccacag atgatgcaat
     1621 actgacaata ttgtcaattt cctcaggtgc gtgtgccata atcggggaac cgccaagtgt
     1681 ttttacgatg tttgcacagt cataaatcgt cacccagttt gttaaatgat gcacaaccgg
     1741 ttttatctct ctaactctat ccagtatttc tggaatatta aaattttcca tcacttcacc
     1801 ctttaataag tcctgtttag acctgaccaa ctttaaacaa aaacataaaa atattataac
     1861 acctatccag gcccactgaa atcttttgcg aatgcaagat aattattaaa gcattgtatt
     1921 gacttaaaca tataagatga tacattatac ctctataaaa caagaggaaa acatggtatt
     1981 aacatacaaa ccagattttg aacagacaaa aattcggtgg agacatttct ggaacaaaga
     2041 agtgtataaa aggccaataa tactggctgg ttgttataaa gaaggcataa acaaacagtt
     2101 tcctgatgtt ttttcccttc ggtattacag aggatataac gaattgtggc aggagcaaat
     2161 tgatttattt aacgaatggg cagaatgctt tgattttgtt ggtgacggaa tcccttcgtt
     2221 ttcgcctgat tttggtcctg accaattttc agcattttat ggtgcaaaac taaggttttc
     2281 tgaacactca aaacatacca gcttgactga gccaattatt ggtaactgga ataaattttt
     2341 acctttgaaa tttgatccat caaatgaaac atttcaaaaa cttgttgctt atgtccgttt
     2401 tatcgcaaag gatgccaagg ggaaatatct ggtttcagac atcgacagac attcaaacat
     2461 agatacattg cttgccctca ggggcgcaga aaaactctgt atggacctgt atgataaccc
     2521 tgaactcatt gaacaagcaa tgctttccct aagaaaagaa ttccctctaa tttctgacat
     2581 gctttacaaa gcaggcatag ataatgatgg tctgggcaca acagtgtcat ggacaggtgg
     2641 tttttatagc gaaaaaaatt tcggtgtcgt acaggctgac tttatttgta tgatgagtcc
     2701 tgaaatgttc aggaaattcg tacttcctgc tcttgaagaa gagattgaat attatgatta
     2761 cagttatttc cacctcgatg gacccggggc attaaaacat cttgatgata tactttcaat
     2821 aaaaaaacta tcggtgttac aatggcaacc aggtgacggt caaaaaccga atttccagtg
     2881 gctcgagatt ttaaaaaagg cacagaaagc aggcaaggcg gttcatgttt ttggaaattc
     2941 aatgcaacca ttgactgctg aaatagtaaa aaaacttcat aaggaacttg acccgacgct
     3001 tgttgtgtat gatggaatac ctataaagga tagaaaagag tttgaaaatc tttcatcatg
     3061 gcttgaaaaa aactaaaaat ctgttgattg ctgcgttaaa aggttatggt tacagataaa
     3121 aacaattaaa atggaagcaa accttcattt accatggagt aaggaaattt caccacctgt
     3181 ccggtcatct atcacatttt attcaaaaat gaatgatgac atgattttta gaaaatgtaa
     3241 aaccatttca atttattgca gaattggata ttgttcgatt tcccttgagt ggacactttc
     3301 ccgcaattta ttcataaccc cgtttctttc aggaacaggc agggctctac caggtaatgt
     3361 tttcatcatt gatattcctg cggaaaaatt acggccgggt ttttatgatt tgaaagtatt
     3421 catagatgtc ggtgaagaca gggaactgga aggtttgtgc acctttggct ttgaaataga
     3481 aaaaataaaa tataaaagta ttttaccttc ggattttaca aaattctggg aatcagccaa
     3541 aaataaaata aacactattc ctcttgaacc agaagaacat ttttttaaga aaatgtcaga
     3601 aaaagaaata gaacaatata atgtgaaaag tgcatgttta ccaccaaagt atgatggcag
     3661 ggtcaggcat aataaaatag aggtctacaa aattagtttt tcttcaatca atggtttgag
     3721 ggtatatgga tggctggcag aacctgtcgg aaaaggccca tttcctggaa tgctcgttct
     3781 accaggagca ggatacaggt cagaacccat tcctgctgaa catgcaagac acggctatgt
     3841 tgcacttgat atacaggttc acaaccagga cgtaaaccag gacaaataca ccaagccaga
     3901 atgggaaaat aaacccgttg attatagtaa tccggaaacg tctgaattct atacaatgta
     3961 tcttaactgt gttcaggcag tcaactattt gagttcaaga aaggacgttg caaaggataa
     4021 aattgtctct gttggcggta gtcagggcgg tcgtctgtct gttattgttg ctgcaatgga
     4081 tacaaggata cgggcaattg ttcctgctat tctctggggc gcaaattttt tgtacca
//
LOCUS       DAORYU010000125         4094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_14640_length_4094_cov_4.728151, whole genome shotgun sequence.
ACCESSION   DAORYU010000125 DAORYU010000000
VERSION     DAORYU010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14640_length_4094_cov_4.728151"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..1102)
                     /locus_tag="PLW07_04855"
     CDS             complement(68..1102)
                     /locus_tag="PLW07_04855"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOL49887.1"
                     /translation="MHQDWHSDFRPEKIKRSIIQFWTVHQKKATFYRQMAVMLKAGIP
                     VNSALNQMKQHSAIANISDRLLKGILTGDTLSQAMAKEQQFFSQLEIKTMSAGEISGN
                     LAELMEKLAVYFESLQDVKYRLVAGMIYPAILLHAAIIIPAVPLLFTKSVFAFFARII
                     PMFILIYGAGFGIFFGYKVLSRPEMTEIRDTLAFKLPLGFGRLFKNIAVIRFLQAFNC
                     LYSAGVSITETIKLAADASGNKIFEKEVSRALQRVENGSGLTEAFADNVFLPPIVLDM
                     FATGQMSGRLDETLDKAVWHLQNQVNLAVEAILKIVPVVVYLLVALYVASIIISFYAR
                     YIGEINSLLE"
     gene            complement(1060..1704)
                     /locus_tag="PLW07_04860"
     CDS             complement(1060..1704)
                     /locus_tag="PLW07_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOL49888.1"
                     /translation="MIQENIEKIMKEVPAHVTVIAATKTRSVEEIKQAIACGITHIGE
                     NYVQEAEKKFKDIGKIVTWHLIGHLQKNKAKKALQIFDIIETVDSFELAREISKYATT
                     KIPVLIEVNSAEEQQKSGVFPEEVESLLKEISTLPNISVQGLMTMGPIVENPENVRPF
                     FTLTKNLFDRLKLLSLPNVEMKILSMGMSDSWRIAIEEGATCIRIGTAIFGPRK"
     gene            complement(1701..2477)
                     /locus_tag="PLW07_04865"
     CDS             complement(1701..2477)
                     /locus_tag="PLW07_04865"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOL49889.1"
                     /translation="MEKNFKYIYGPVSSWRLGSSLGIDQLSQEKKICSFDCVYCQVGR
                     TGIFTCERSIYVPAESIIEELKQLPDLEIDYYTFSGRGEPTLAKDFKEVANWIKQVKK
                     GKCAILTNSTTISDESVKQDLCVMDLVSFKIDAANQKTFEIVNRPCCGIKLENILNHI
                     ADFKKMYKGCFTIQVMAIQENVKEMVQIAEICKKLSPDIVYLNTPLRDSPVAPISETS
                     FKEVEKLFEGMRCISVYSAHKKAVKPISMKDTMRRRGKKI"
     gene            complement(2479..3882)
                     /locus_tag="PLW07_04870"
     CDS             complement(2479..3882)
                     /locus_tag="PLW07_04870"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOL49890.1"
                     /translation="MNIVLVVFDSLRKDCIGFYGKPFWGRVFTPHLDAFAQESVVFTN
                     AFPESLPTLPARRALYTGRRTYPFVNGDFRLKGDFVGAAGWGPIPEDQPTVAEILRET
                     GYRTTLISDVYHMFKPSKNFWRGFDQWTFLRGQETDPARSGPRLTKEEINKWLPEDLQ
                     TEGSIRFIQQCIMNMHDRWTNEEEFFAPRVFREAAKWLQQNQDAEKFFLVIESFDPHE
                     PWIVPQYYRKLYLKEDGPEQIKSGYSEQQLGSYLLKRTQANYSGEATMCDRWFGFFIE
                     SMKLMGLLDNTIVIVTSDHGHSIGDRSYMGKRGYPSMPEVYEIPLMIRFPEAKYKRKI
                     CDVFVQHHDIAAQILHTAKVKNLPVLDGIAFLEKALKNQPIRDHVTVGWGPNPTVITA
                     EWWLNFKMDMTGILLYRRKGKDTFKKNYAAQYPNVVKNLMDIAVKDAGGSFPQWLVEM
                     AKKQMDAPGCSDLAARK"
BASE COUNT         1160 a          884 c          768 g         1282 t
ORIGIN      
        1 aaccctatca atgaaattgt ttcagcaacc cagatgatat acaggtgttt tttccagtca
       61 tccattgtta ttccagaagc gagttgattt ctccgatgta tcttgcgtaa aaagaaatga
      121 tgattgatgc aacatacaga gccacaagaa gatacaccac aacaggcaca atttttaaaa
      181 ttgcctctac tgcaaggttg acctggtttt gaagatgcca gacggcttta tcaagtgtct
      241 catctaacct gccactcatc tgccctgttg caaacatatc aagaacaatc ggtggtaaaa
      301 atacattgtc tgcaaatgcc tctgttaaac ctgaaccatt ttcaaccctt tgaagggctc
      361 tgctgacttc tttttcaaaa attttgttgc cagaagcgtc tgcggcaagt ttaatggttt
      421 cagtaattga aacccctgca gaataaaggc agttgaatgc ctgcaaaaat ctaatgacag
      481 cgatattctt gaaaagtcga ccaaacccaa gtggtaattt gaaagcaagc gtgtctctga
      541 tttctgtcat ctctggtcgt gaaagaactt tgtatccaaa aaaaatccca aaaccagcgc
      601 cataaatcag aataaacatt ggaataattc ttgcaaaaaa agcaaacaca gattttgtaa
      661 aaagaagcgg gacagcagga ataatgatgg ctgcgtgaag aagaattgca gggtatatca
      721 ttcctgcgac cagccgatat tttacatcct gcaatgattc aaaatacaca gcaagttttt
      781 ccatcagttc tgcaaggttt ccagaaattt ctcctgctga cattgttttg atttcaagtt
      841 gactgaaaaa ttgttgttct tttgccattg cctgagaaag cgtatcgcct gtcaatatac
      901 cctttagcag tctatctgag atatttgcaa ttgcagaatg ctgtttcatc tggttcaaag
      961 cagaattgac aggaatgccg gctttcagca tcacagccat ctgcctgtaa aaggtggctt
     1021 ttttttgatg cacagtccag aattgaatga tggaacgctt tattttctcg ggccgaaaat
     1081 cgctgtgcca atcctgatgc atgttgctcc ttcttcaatg gcaatgcgcc aggaatcgct
     1141 cattcccatt gaaaggattt tcatttcaac attaggtaga gacaaaagtt tcaaacgatc
     1201 aaaaaggttt ttcgtgagtg taaaaaatgg tctgacattt tcaggatttt caacaatggg
     1261 tcccattgtc atcagccctt gcacagaaat attgggcaat gtggagattt ctttaagcaa
     1321 tgactcaact tcttcaggaa aaacacctga tttctgttgt tcttctgcgc tgttcacttc
     1381 aatgagcaca ggaatctttg ttgttgcgta ctttgaaatt tcccttgcaa gttcaaagga
     1441 atccacggtt tcaatgatat caaaaatctg aagcgccttt ttcgccttgt ttttttgcag
     1501 gtgtccaata agatgccatg tcacaatttt tccaatgtcc ttgaattttt tctctgcttc
     1561 ctgaacataa ttttcaccga tatgcgtaat cccgcacgca atcgcctgtt ttatttcttc
     1621 cactgaacgg gtttttgttg cagcaattac tgtcacatgt gctggaactt ccttcataat
     1681 tttttcaatg ttttcctgta tcatatcttc ttacccctcc gtctcatcgt gtctttcatg
     1741 ctgatgggct tcactgcctt tttatgcgca gaataaacag agatacagcg cattccttca
     1801 aagagttttt ccacttcctt aaacgatgtc tcagaaattg gtgcaacagg gctatccctg
     1861 agcggcgtat tgagatacac aatgtctgga gaaagttttt tgcaaatttc tgcaatttgg
     1921 accatttctt ttacattctc ttgaattgcc atcacctgaa ttgtaaaaca acccttgtac
     1981 atttttttga aatctgctat gtgattaagg atgttttcca gtttaatacc acaacatggc
     2041 cgattaacaa tttcaaaagt tttctggttg gctgcatcta ttttgaacga gacaaggtcc
     2101 atcacacaca ggtcctgttt cacagattca tctgagattg tggtactatt ggtaaggatt
     2161 gcgcacttac cttttttgac ctgttttatc cagtttgcta cttccttgaa atcctttgca
     2221 agcgttggtt cgcccctacc tgaaaacgtg taataatcaa tctcaaggtc tggcaattgt
     2281 ttcaattctt caatgattga ttctgctggc acataaatgc ttctttcgca ggtaaaaatc
     2341 cctgttcttc cgacctgaca gtagacgcag tcaaacgaac agattttttt ctcctgggat
     2401 aactggtcaa tgccaagaga acttccaagc cgccaggaac tcacaggccc ataaatgtac
     2461 ttgaaatttt tttccatgtt atttccttgc agcaaggtcg ctgcagccag gtgcatccat
     2521 ttgttttttt gccatttcaa caagccattg tggaaaactt ccacctgcat cttttacggc
     2581 aatgtccatc aaatttttta caacattcgg gtattgtgca gcataatttt ttttgaaggt
     2641 atcctttcct tttctcctgt aaagaaggat accagtcata tccattttga aattcaacca
     2701 ccactcggca gtgatgactg ttggatttgg gccccagcca actgtgacat ggtctcttat
     2761 tggctgattt tttaatgcct tttcaagaaa cgcaatgcca tcaagaactg gtaaattttt
     2821 tacctttgct gtatgaagta tttgcgcagc aatgtcgtga tgctggacaa aaacatcaca
     2881 gatttttctt ttgtattttg cttcaggaaa ccgtatcatc aagggtattt cgtaaacctc
     2941 aggcatagaa ggatatcccc gttttcccat gtaagacctg tcgccgatag aatggccgtg
     3001 gtcagacgtc acaatcacga tggtattatc aagcaatccc atcaatttca tactttcaat
     3061 gaaaaaccca aaccacctgt cgcacattgt tgcttcacca ctgtagtttg cctgagtcct
     3121 tttaagaaga taactgccaa gttgctgttc agaataacct gattttatct gttcaggtcc
     3181 atcttctttg agatacaact ttctgtaata ctgcggcaca atccatggtt cgtgcgggtc
     3241 aaatgattca atcacaagga aaaacttttc agcatcctga ttttgctgaa gccattttgc
     3301 tgcctcacga aagacccgtg gagcaaaaaa ttcttcttca ttcgtccatc tatcgtgcat
     3361 gttcataatg cattgctgga taaacctgat actgccttct gtctgcaagt cctctggaag
     3421 ccatttatta atctcttctt ttgttaatcg tggtccagaa cgggcagggt ctgtttcctg
     3481 tccacgcaaa aatgtccact ggtcaaatcc gcgccagaaa ttttttgacg gtttgaacat
     3541 atgataaaca tctgatatca atgttgtgcg atacccagtt tcccttaaaa tttctgcaac
     3601 tgttggctgg tcttctggaa taggacccca tccagcagcg ccaacaaaat ccccttttaa
     3661 tctgaaatct ccattgacaa acgggtaagt tcttctacca gtatacaggg ctcttcttgc
     3721 tggaagcgtt ggcagggatt caggaaaggc atttgtaaaa acaacagatt cttgagcaaa
     3781 cgcatcaaga tgcggagtaa aaaccctgcc ccagaatggt ttaccataaa aaccaataca
     3841 atcctttctc agagagtcaa aaacaacaag cactatgttc attgtttaac tcctgtttga
     3901 taaagacatc tggaccagtg atgagaattt taacccgaaa aacaattgat gacaaattga
     3961 agtaaagtat ttcacctata ctgctgccca ttaaggggaa atggaaaata aggtagaata
     4021 gagtggtctg aaaggggaat atactgctcc ccattaagtg gagatggaaa ataaggtaga
     4081 atagagtggt ctga
//
LOCUS       DAORYU010000126         4093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_14642_length_4093_cov_5.706786, whole genome shotgun sequence.
ACCESSION   DAORYU010000126 DAORYU010000000
VERSION     DAORYU010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14642_length_4093_cov_5.706786"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /gene="recR"
                     /locus_tag="PLW07_04875"
     CDS             <1..603
                     /gene="recR"
                     /locus_tag="PLW07_04875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOL49891.1"
                     /translation="TILMETYPPVIRAIVEQLKKLPGIGTKSAERIVECLMKMEEPEV
                     LAFADNLKKLKHNIRLCKQCFSITENEVCDICSDTSREKKLAVVEEYKDAVALEKAGF
                     RGKYHILGGRINPLEKIGPENLSINYLLERLKKEQFDEIIIATNPTAEGESTAMYLVD
                     LLKKQSYPVTRLATGIPVGAEIEYIDPETIRKSLEHRFKP"
     gene            624..1505
                     /locus_tag="PLW07_04880"
     CDS             624..1505
                     /locus_tag="PLW07_04880"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOL49892.1"
                     /translation="MEYVVYRFFCFLGMHLPDWLAYFIGDVIAELRYLFTPGLRKTVR
                     SNIKQVFTYKSQVEHFQFDEQDICRTVKQIYKNFGMYMVEFFQIPKWNRQWVQERVEL
                     VNIHYLDRALRQSKGAIVLTGHLGNWELAGIVTSLLGYNLSAVALPFKNEKMATIFVR
                     RRKLKGVNVILTGANPKEYLRAFRKNSVIAILGDRLFTEKGMRVTFMGKETFLPRGPA
                     TLAARWNAGYLAGFLIRKKKGKYMLVFDKPIEIEGTDENQKVQYLVEKGTEYLEKYIL
                     KYYDQWLNFSPMWNDNG"
     gene            1519..>4093
                     /locus_tag="PLW07_04885"
     CDS             1519..>4093
                     /locus_tag="PLW07_04885"
                     /inference="COORDINATES: protein motif:HMM:NF020121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HOL49893.1"
                     /translation="MIKIFLFCLVFLSFSISVADIHPATQFRSADESVIACPDGTIFC
                     EAEEFKVIKGAWQAKHWGENYFAATFANTFLSRKAFLGAPEQCDESIATINAKIDRPG
                     EYLVLVRYEAPYRFETQFRIRIEQNGKTLMDRLYGARDNLKIWAFGEKLKKEVAWSWG
                     AVENIVWEGHDAAVQLQPGLIKISLMAGNQPQPAAKRNIDIVMITPDRQQVNMRIEKE
                     GYLPLDGWLTQSGDVWMKVKNQSADNITVKSLSFPGGPFQQHSPYWVHLRTWKPISVS
                     VDAGQQTDWIEVGSSMDTLNDGQWGFGVSGACVLEFGVKNASGNIGKIREFTLFGASQ
                     LNLVGLADTRYSKKIQSEQEALGDLVENLKKIKSHGKMPEQTLIFAGTKIKEFAQLFG
                     LATDPDIHVDWRGKNPAQLEAMCTSLTSQQREKIRVVSLGDEIGLPAPDARAAKEGFV
                     EYLKKQSVNPEDIDPQGNRDNLVYNPSQKMKTENPALYYWSKKFQHYYGIQSIKQLTD
                     TLRKYLPDAGIGANFSPHHGGYQHSYLGEVFQWVHCFREDGLTMPWAEDYIWQVPVGS
                     PQMNEINLDLFRAGLRGKTDRKIHYYVMPHWPGNTPNMWKRQFYAALAHGAKIFNLFE
                     FHPVYMAYTENHVSHFDTYAMILKSFREYGLFEDIVQQGNVRQADAGLWFSETADIWS
                     DNEGSFAAAKRALYIAIRNNQIPLDFIVEQDALDGTIDKYKTLFITDRHISLDASQKL
                     EQWVLNGGSLFMTAGAGMFEQYNRKNTVFNRLLGFEQIEILEPENSRVEFIKQDLPFA
                     KPASEIVEINGSKVSIPVFGVITKIQPGKTTTISGKFSDGSPAVVSIKKGKGQIIYCA
                     FL"
BASE COUNT         1361 a          711 c          927 g         1094 t
ORIGIN      
        1 accattctga tggaaacata cccacctgta atcagggcaa ttgttgaaca gttaaaaaaa
       61 ttgcccggga taggcacaaa aagcgcagaa agaattgttg aatgcttgat gaaaatggaa
      121 gaacctgaag tgctggcatt tgctgacaat ctaaaaaaac ttaaacacaa catcagattg
      181 tgtaagcaat gtttttcaat tacagaaaat gaagtgtgcg atatttgcag tgatacttcg
      241 agagagaaaa aactggcggt tgttgaagaa tataaggacg cagtggctct tgaaaaggcg
      301 ggatttcgcg gcaaatacca tattcttggt ggaagaatca atcctcttga aaaaatcggg
      361 ccagaaaatc tttccatcaa ttatttgctg gaacgtctca agaaagagca gttcgacgaa
      421 attatcatag caacaaaccc cactgctgaa ggcgaaagca cagcaatgta ccttgttgat
      481 cttcttaaaa aacaatcata tccagtcacg cgtcttgcca caggtattcc tgttggtgca
      541 gaaattgaat acatagaccc tgaaacaata agaaaatcac ttgaacacag gttcaagcca
      601 taacatttca gtggatacag acgatggaat atgttgttta tagatttttc tgtttcctcg
      661 gtatgcacct tcctgattgg cttgcatatt tcattggtga tgtgattgct gaattaagat
      721 atttgtttac tcctggtttg agaaaaactg tcaggtcaaa cataaaacag gtatttactt
      781 ataaatcaca ggttgaacac tttcagtttg atgaacaaga catttgtaga acagtgaaac
      841 aaatatacaa aaattttgga atgtacatgg tggaattttt ccagattcca aaatggaatc
      901 gccaatgggt acaggaaaga gttgaacttg tcaatatcca ttaccttgac cgggcgctac
      961 gccagtcaaa aggcgctatt gttctgacag gacatcttgg gaattgggaa cttgctggta
     1021 ttgttacaag tttgctcggg tataacctgt ctgctgtcgc gctgccattc aagaatgaaa
     1081 aaatggcaac aatttttgtc agacgaagaa agttaaaagg tgtcaatgta atactgacag
     1141 gcgcaaatcc aaaggaatac ctcagggcat tcagaaaaaa tagtgtgatt gcaattcttg
     1201 gagaccgtct cttcacagaa aaagggatgc gtgtaacatt catgggaaaa gaaacgtttc
     1261 ttccaagggg accagcaaca cttgctgcac gatggaatgc gggttatctt gcagggtttt
     1321 tgataagaaa gaaaaaagga aaatatatgc tggtttttga taaacccata gaaattgaag
     1381 gtacagatga aaatcaaaag gttcagtatc ttgttgaaaa gggtacagaa tatcttgaaa
     1441 aatacattct gaaatactat gaccagtggt tgaatttttc accaatgtgg aatgataacg
     1501 ggtaaaaaag ggggaatgat gataaaaata tttctttttt gtttggtctt tctttcattc
     1561 agtatcagtg ttgctgatat tcatccagca acacaattca ggtctgctga tgaatctgtt
     1621 attgcctgcc ctgacggtac gattttttgt gaggcagagg aatttaaggt tatcaagggc
     1681 gcatggcagg caaaacactg gggagaaaac tattttgcag caacatttgc caataccttt
     1741 ctttcaagga aagcatttct tggagcacct gagcaatgcg atgaatctat cgcaaccatc
     1801 aatgcaaaaa ttgaccggcc aggcgaatat cttgttcttg tcaggtatga agcaccatac
     1861 agatttgaaa cgcagttccg gataagaatt gaacaaaatg gaaaaacgtt gatggaccgg
     1921 ttatatggtg ccagagataa tctgaaaata tgggcatttg gtgaaaaact caagaaagaa
     1981 gttgcatgga gctggggagc agtagaaaac attgtctggg aaggtcatga tgctgctgtg
     2041 caactgcaac cagggctgat aaaaatttct ttgatggcag gaaatcaacc tcagccagca
     2101 gcaaaaagaa atatcgatat tgtgatgatt acacctgaca ggcagcaggt aaatatgaga
     2161 atagaaaaag aaggatatct gccgcttgat ggatggctaa cacagtcagg cgatgtgtgg
     2221 atgaaagtga aaaaccaatc tgctgataac attacagtga aaagtttatc gtttcctgga
     2281 ggaccatttc aacagcattc tccatactgg gttcatcttc gcacctggaa gccaatcagt
     2341 gtgtctgtgg atgcagggca acaaactgac tggattgagg ttggttcgag catggataca
     2401 cttaatgatg gacagtgggg atttggtgta tctggagcat gcgttctgga attcggggta
     2461 aaaaacgctt ccggtaatat tggaaaaatc agagaattta ctttgtttgg agccagccag
     2521 cttaatcttg ttggtcttgc tgacacaaga tattcaaaaa aaatacagtc agagcaagaa
     2581 gcccttggag accttgttga aaatcttaaa aagataaaaa gccatggaaa gatgcctgaa
     2641 caaacactga tatttgcagg aacaaaaatc aaggaatttg ctcaattatt cggacttgcg
     2701 acagaccctg atattcatgt tgactggaga ggtaaaaatc cagcgcaact tgaagcaatg
     2761 tgtacgtccc ttacaagcca gcagagagaa aaaatcaggg tggtcagttt aggcgatgaa
     2821 atagggctac cggcacctga tgccagggca gcaaaggaag gatttgttga atatctgaaa
     2881 aaacaatcag taaaccccga agatatcgac ccgcagggta accgggataa tctcgtgtat
     2941 aatccttccc agaaaatgaa aactgaaaat cctgcattgt attactggtc gaaaaaattt
     3001 cagcattatt atggcataca gtcaatcaaa cagttgaccg ataccttgcg aaaatatctt
     3061 cctgatgcag gtattggagc aaatttctcg ccccatcatg gaggatatca acattcatat
     3121 cttggagagg tgtttcaatg ggttcattgt ttcagagaag atggattaac aatgccatgg
     3181 gcagaagatt atatctggca ggtacctgtg ggtagcccac agatgaatga aatcaatctt
     3241 gaccttttca gggcaggact gagaggaaaa actgacagaa aaatacatta ctatgtaatg
     3301 ccacactggc caggcaatac gccaaatatg tggaaacggc aattttacgc tgcgcttgct
     3361 catggagcaa aaattttcaa tctttttgaa tttcatcctg tgtatatggc atacactgaa
     3421 aatcatgttt cacattttga tacatatgca atgatactga aaagtttcag agaatacgga
     3481 ttgtttgagg acattgtcca gcagggaaat gtgcggcagg cagatgcagg attatggttc
     3541 agtgagaccg cagatatctg gtcagataat gagggttcat ttgcagcggc aaagcgtgcc
     3601 ctgtacattg caataagaaa taaccagatt ccattggatt tcattgttga acaggatgcc
     3661 ttagacggaa caattgataa atataaaaca ctcttcatta ctgacagaca tatttcactg
     3721 gatgcatcac aaaaacttga acagtgggtg ttaaatggtg ggtcattgtt tatgacagca
     3781 ggcgcaggga tgtttgagca gtataacagg aaaaatacag tattcaacag gttgcttgga
     3841 tttgaacaga tagaaatatt agagccagaa aatagcaggg ttgagtttat caaacaggac
     3901 ctgccctttg ccaaacctgc gtctgaaatt gttgaaataa atggatcaaa agtttccata
     3961 ccagtttttg gcgtaattac aaaaattcag ccaggaaaga ccactactat ttcaggtaaa
     4021 ttttcagatg gttctcctgc ggttgtttca ataaagaaag ggaaaggaca gattatttat
     4081 tgcgcatttc ttc
//
LOCUS       DAORYU010000127         4041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_14877_length_4041_cov_4.550176, whole genome shotgun sequence.
ACCESSION   DAORYU010000127 DAORYU010000000
VERSION     DAORYU010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14877_length_4041_cov_4.550176"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..367)
                     /locus_tag="PLW07_04890"
     CDS             complement(89..367)
                     /locus_tag="PLW07_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOL49894.1"
                     /translation="MPSYDYECKKCGHSYRKFQKMTDEPDKICPECKGEVRRLIGLGG
                     GVIYKGSGFYTTDYRSPEYTRRAKEESGAGSGSSCCSCSSKSCSTCGK"
     gene            complement(474..1400)
                     /locus_tag="PLW07_04895"
     CDS             complement(474..1400)
                     /locus_tag="PLW07_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOL49895.1"
                     /translation="MPSIKELAKRKEKFVSGHRLCPGCGAGVVARLVMNAIDKPVVVG
                     CATGCLEVSSTIFPYSSWDTPFIHSAFENAAATVSGVETAYRSLKKQGKIDKDVVFVA
                     FGGDGGTYDIGLQSLSGAAERGHKMLYVCYNNQAYMNTGIQRSSATPKGASTTTSPAG
                     KVIPGKIQFSKDIMGIMIAHNIPYAAQTIPGRWNDITNKVEKAINTDGFSFINVLSPC
                     RLGWPHKPEIAMEITRLAVETCFWPLYEVIDGKYKLNYKPKEKKPIIEWLKIQGRFAH
                     LLKPGNEKIIEGIQQEVDRRWENLLKKCESSV"
     gene            complement(1420..2601)
                     /gene="porA"
                     /locus_tag="PLW07_04900"
     CDS             complement(1420..2601)
                     /gene="porA"
                     /locus_tag="PLW07_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HOL49896.1"
                     /translation="MPSLKKVTKTGNEVFAEAMRQINPDVVAAYPITPATEIVQIFSQ
                     FVADGLVKTEYIAVESEHSAMSACIGSSAAGARTMTGTSAQGLALMWEMLYIAAGLKL
                     PIIMAVVNRALSAPINIHCDHSDTMGARDSGWIQIYSENVQEAYDNLIQAVRIAEHPD
                     VRLPVMATTDGFILSHCLEVAEILNDSDVQSFVGIPPERPTVLDFDKPITLGALDLQD
                     YYFEHRRQIAEAMTRSIKVINDIADKYAKLSGRKYEFFEKYQLDDAEYAIVLIGSTAG
                     TAKDVVDKLRSQGKKVGLLKIRVFRPFDYMKVRQTLENIKVIGVMDRADGLNAYCGPL
                     YTEICTSLYESEKKPALANYIYGLGGREVKEQDIETIFDDLFKIGKEGKTVTTKYIGV
                     R"
     gene            2677..3966
                     /locus_tag="PLW07_04905"
     CDS             2677..3966
                     /locus_tag="PLW07_04905"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HOL49897.1"
                     /translation="MNTVIQIALDFVDIDRALRVAREAVKGGANWLEAGTPLIKSCGL
                     DAVRMLKKEFPQHSVVADMKVLDAGRTEVEIAAKSGADIVVVMAHASDSTIQEAIEAS
                     RNYGAKILVDFMEDACNIERIKTIAGFHPDFIGVHIPIDQQMTGNISFDFLKKISKIV
                     SVPLAIAGGLNSENVTDAIKAGASILIIGGAVTKSENAEKAVRIIKKAIETDTPIKSE
                     LFKRYTADNLDEVFSKVSTANLSDAMHRSGWIPDIKPVSCTGKFFGTAITVKTYPGDW
                     AKPVEAVDIARAGQVVVIDACGLPPAVWGELATHSASVKKLAGVVIHGAIRDVEEIKN
                     LKFPAFATLCCPQAGEPKGLGEINTPIQIGPIKILPGDWIVGDEDGVVCIPSQKAAEI
                     ANRAMDVLEKENRLRAEIKNGGTLSEITHLLRWEKQK"
BASE COUNT         1169 a          849 c          816 g         1207 t
ORIGIN      
        1 acctacaacc ttgcggttaa cagccgcttg ctccaccgtt gagctacgcc ggaatacctt
       61 tttcatcttc tgccattagt ggcagagttt atttaccaca tgttgaacaa ctttttgaag
      121 aacaactaca acatgatgaa cctgaacctg ctccactttc ttcttttgct cttcttgtat
      181 attcagggct tcgatagtca gttgtataaa aacccgaacc cttgtatatt acgccgccac
      241 caagtccaat gagacgcctt acctctccct tacactcagg gcagatttta tccggctcat
      301 cagtcatttt ctgaaacttc cgataggaat gcccgcactt tttgcattca taatcataac
      361 ttggcatttc tatccctcct cctgtttata aaaaatttta actttttttg ctaaattggt
      421 caaactttaa aacttaaaaa tttagattat ttttatttct tgctggattc agtttatacg
      481 gaggactcgc attttttcag taaattctcc cagcgtctat caacttcctg ctggattccc
      541 tcaataattt tttcgttgcc gggctttaat aaatgcgcaa atctcccctg aatttttaac
      601 cactcaataa tgggtttctt ttcttttggt ttataattga gtttatattt gccatcaata
      661 acttcgtaca gtggccagaa acatgtttca accgcaagtc gagtgatttc cattgcaatt
      721 tctggtttat gaggccagcc gagacgacac ggagaaagca cattgataaa tgaaaaacca
      781 tctgtgttaa tggctttctc cactttattt gtaatatcat tccatcttcc aggaatggtt
      841 tgtgcagcat aagggatatt gtgggcaatc atgattccca taatatcctt tgaaaactgg
      901 atctttcccg gaatgacttt tcctgcaggc gatgtggttg ttgaagcgcc ttttggagtt
      961 gctgaagaac gttgaatacc agtattcatg tatgcctgat tgttatagca aacataaagc
     1021 atcttatgtc cgcgttctgc tgcgccagaa agggattgaa gacctatatc atatgttccg
     1081 ccatcaccac caaaagcaac aaaaacaacg tctttgtcaa ttttaccctg tttctttaat
     1141 gacctgtagg ctgtttcaac cccacttact gtggctgcag cgttctcaaa ggccgagtgg
     1201 ataaaaggag tatcccaact tgagtaagga aaaatcgttg atgaaacttc aagacaccct
     1261 gttgcgcagc cgacaaccac aggtttatct atggcgttca ttacaagccg agccacaacc
     1321 cctgccccac aacccgggca gagcctgtga ccactgacaa acttttcttt tctttttgca
     1381 agttctttaa ttgatggcat tttctcaccc ctgattgaat tatcttactc cgatatattt
     1441 tgttgttact gtttttcctt cttttccgat tttgaataag tcgtcaaata ttgtttctat
     1501 atcctgttct tttacttctc tgccacccag accataaata taatttgcaa gtgctggttt
     1561 cttttcagat tcataaagag atgtgcagat ttcagtgtaa agtggaccac aatatgcatt
     1621 aagtccatct gccctgtcca ttacaccgat gacttttata ttttccagtg tctgtctaac
     1681 cttcatataa tcaaatggtc tgaaaaccct gattttcaac aaaccaactt ttttaccctg
     1741 ggaacgtagt ttatctacaa catcttttgc tgttccagcg gttgaaccaa taagaacaat
     1801 ggcatattca gcatcatcaa gctggtattt ttcaaaaaat tcgtattttc taccagaaag
     1861 tttagcgtat ttatcggcaa tgtcattgat taccttgata cttctggtca ttgcttctgc
     1921 aatctgtctc ctgtgctcaa agtagtaatc ctgaagatca agggcaccaa gcgtgatggg
     1981 cttatcaaaa tcaagtactg tgggtctttc gggaggaata ccaacaaacg attggacatc
     2041 gctgtcatta agaatttcag caacttcaag acaatggctt aaaataaaac catcggttgt
     2101 tgccataaca ggaagtctga catctggatg ttctgcaatt ctaactgcct gaataagatt
     2161 atcatatgcc tcctgaacat tctcagaata aatctgtatc cagccactat ctcttgcgcc
     2221 catggtatct gagtggtcgc agtgtatatt gattggcgca gataatgccc tgttcacgac
     2281 tgccattata atcggcaatt tcaaaccagc cgcgatataa agcatttccc acatcaatgc
     2341 aagtccctga gcagatgttc ctgtcattgt tcttgcgcca gcagcagatg atccaataca
     2401 ggcgctcatt gcactgtgtt cactttctac tgcaatgtat tcggttttta ccagcccgtc
     2461 tgcgacaaac tgagaaaaaa tctgaacgat ttctgttgct ggtgtgatag gatatgcagc
     2521 gacaacatca gggtttatct gccgcattgc ttcagcaaaa acttcgtttc ctgtttttgt
     2581 aacttttttt aaagagggca tcttggctcc tttcaaaaaa tcaaaaaaaa tggtaagatt
     2641 taataaataa taacgcaaaa taaaaaaatg tcaaaaatga acactgttat tcaaatagcc
     2701 cttgattttg ttgatattga ccgtgcatta agggtcgcca gagaagccgt gaagggtggt
     2761 gctaactggc ttgaagcagg tactcctctg attaaatcct gtgggcttga tgctgtcaga
     2821 atgctaaaaa aagaatttcc acaacattct gttgttgcgg atatgaaggt gcttgatgca
     2881 ggaagaaccg aagttgaaat tgcggcaaaa agcggcgctg atatcgttgt tgtgatggcg
     2941 catgcttctg acagcaccat tcaagaagcc attgaagcct caagaaatta tggagcaaag
     3001 attctggtgg attttatgga agatgcctgc aatattgaaa ggataaaaac aattgcaggg
     3061 ttccatccgg atttcattgg tgttcatatt cctatagacc agcaaatgac aggcaatatt
     3121 tcttttgatt tcttaaagaa gatttccaaa attgttagtg tgccacttgc aattgctggt
     3181 ggactgaact ctgaaaacgt cactgatgcc ataaaagcag gcgcaagcat tctcattatt
     3241 ggtggcgctg taaccaagtc agaaaatgct gaaaaggcag taagaataat taaaaaagcc
     3301 attgaaacag acaccccaat taaatcagaa ctgttcaaaa ggtacactgc agacaatctt
     3361 gatgaggtgt tttcaaaggt ttcgactgca aatttgtcag atgccatgca tcgaagtggc
     3421 tggattccag atataaaacc agtaagttgt acaggaaagt ttttcggtac ggcaattact
     3481 gtgaagacct atccagggga ctgggcaaag cccgtagagg ctgtggacat tgcccgggca
     3541 ggacaggttg tggtcatcga tgcctgcggt ctgccaccag ccgtatgggg agaactggca
     3601 acccacagtg catcagtaaa gaaacttgca ggtgtggtta ttcatggcgc aataagggat
     3661 gtggaggaaa ttaagaatct taaattccct gcatttgcaa cgctatgctg tcctcaggca
     3721 ggagaaccaa aaggacttgg cgaaatcaat accccgattc aaataggacc cataaaaatt
     3781 ttacctggtg actggattgt tggagatgaa gatggcgtgg tgtgcatacc ttctcaaaag
     3841 gcagcagaga tagccaatag agcaatggat gtgcttgaaa aggaaaacag gttgcgagca
     3901 gaaatcaaga atggtggtac attgagtgag ataacacatt tgttgaggtg ggaaaagcaa
     3961 aaatagggga atggatggaa agacagaagc attatttcac atacgctgcg gtgcagacaa
     4021 aactacggat aaatccgctg c
//
LOCUS       DAORYU010000128         4021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_14978_length_4021_cov_3.803328, whole genome shotgun sequence.
ACCESSION   DAORYU010000128 DAORYU010000000
VERSION     DAORYU010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14978_length_4021_cov_3.803328"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="PLW07_04910"
     CDS             <1..675
                     /locus_tag="PLW07_04910"
                     /inference="COORDINATES: protein motif:HMM:NF024045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOL49898.1"
                     /translation="KSSLLNLLVEEDKAIVSEIPGTTRDSIEAIINIQGVPFTIVDTA
                     GIRHHGSGIEKIGVERAKKWMKKADMILVVFDSARPLDNLDLQILKEAKNKPHIIVLN
                     KCDLQSRLDKKILHENVIDSPVVKISCLTGQGLNQLHSAIMENFYSGFCQMEDIQFAL
                     NLRQQNSLEKAIKTLEELRNVSNKSTDIVAEMVRYSILCLDEISGNNISDQVIDEIFS
                     RFCIGK"
     gene            690..1445
                     /locus_tag="PLW07_04915"
     CDS             690..1445
                     /locus_tag="PLW07_04915"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOL49899.1"
                     /translation="MKRMVISLGLIAVMFFSGCATYYNPVTGKQETTAYTEQDEIDMG
                     IALDKKICSEFKIVENPEKAFPGFTSICSRVARNNDRNHLNYTFRVIENKEVNAFSIP
                     GGFVYVYTGLLQQVNSPDELACIVGHEIGHICNRDGVHRLEKQILYSIPASILFGSGR
                     QKAIQQAADAVFTLSMLKYSRSQEIEADTLGMTYAYRAGFNPEGMISFFKKMEELEKN
                     QPSIPLTFLRDHPETKERIKNAQSVINNLKQNK"
     gene            complement(1450..2097)
                     /locus_tag="PLW07_04920"
     CDS             complement(1450..2097)
                     /locus_tag="PLW07_04920"
                     /inference="COORDINATES: protein motif:HMM:NF014762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOL49900.1"
                     /translation="MDRELEDILEEIWKKRELKESFVTGFDKEKMEHLRKMGLVEVSE
                     RGAVLTKQGEEIAKCLVRRHRLWEVFLHDFLGMEEFNEMAGIIEHDAKGNIDEALCDF
                     LGHPQQCPDGKPIPRGLCCGRRLKRRAGRGFFHQMGVLRLCDVPRGTRVKVVCLRGKK
                     FPRLSGYGIVPGCMIYVQETFPGFIIRIDQTEIALDKESCADVLVQLIDQQDEHV"
     gene            complement(2107..>4021)
                     /gene="feoB"
                     /locus_tag="PLW07_04925"
     CDS             complement(2107..>4021)
                     /gene="feoB"
                     /locus_tag="PLW07_04925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOL49901.1"
                     /translation="KKIILVGNANVGKSVIFNLLTGSYAIVSNYPGTTVEISQGKAKI
                     GNENYVVIDTPGIIDFLGKTEDQQVTREILLENPDAIVVLVIDAKNLKRGLVLSFTLC
                     ELGVRHVVVLNMMDEANDRGIFIDWKKLSEILGVPVVPMIATEYVGLHQLKESITRAG
                     KGKYKIVYHDDVENMIANIKKNLPDTSFHSRGIASLLAGATDSIHDICKKFPHVNEKL
                     IESIWERFQSSMSQPLNYLIVTSQIKESEKIIDMVATKSKPRGGKFQQFMSRVTIHPI
                     SGVLIALLVLFFMYLIVGKFGAVVLVDLIEGKLFGRIINPFFQFIFERTPQLVKDFFV
                     GEFGIITMALTYGIGIVLPITGTFFLFFGFLEDCGYLPRLAFLMDRIFKSIGLTGKAV
                     LPLVLGFGCGTMATLTTRILDTRKEKIITTVLLAVAIPCSAQLGVVLGVLGSISPFAL
                     LAWMVTIVIVMIIVGSALNLLVPGNAIFSMEIPPLRVPGIRNIVLKTGRRILWYLKEA
                     LPFFVLGTMILFLLDRAGVLSYFVSWISYPVIAMGLPLETSKVFVMGFLRRDYGAAGM
                     FDLFSKGILNPQQAVVSAVTLTLFVPCIAQFFVMIKERGLFAAILISIFAMVIAFVVG
                     FLLSAVLSASGLF"
BASE COUNT         1328 a          878 c          692 g         1123 t
ORIGIN      
        1 aagtccagtt tactcaacct tcttgttgaa gaagataaag caatcgtatc tgaaatacca
       61 ggtaccacaa gagattcaat agaagcaatc ataaacattc agggagttcc attcacgatt
      121 gttgatacag caggaattag acatcacggt tcaggcattg aaaaaattgg tgtggaacgc
      181 gcaaagaaat ggatgaaaaa ggctgacatg atactggttg tgtttgattc agcaagacca
      241 cttgataatc ttgacttaca aattctaaaa gaggcaaaaa ataaaccaca tatcattgtt
      301 cttaataaat gcgatcttca gagccggctt gataaaaaaa ttctacatga aaatgttatt
      361 gattcacctg ttgtaaaaat atcctgcctc accggacagg gactgaatca gcttcattca
      421 gcaattatgg aaaattttta ttcaggtttc tgtcagatgg aagacattca gtttgcgctt
      481 aatctcagac aacaaaactc gcttgaaaaa gccataaaaa cacttgaaga attaagaaat
      541 gtcagtaata aaagtactga cattgttgca gaaatggtaa gatatagtat cctttgtctt
      601 gacgaaataa gtggaaacaa tatttctgac caggttattg atgaaatttt cagcagattc
      661 tgtattggta aataacagga ggaacacata tgaaaagaat ggttatttct ctgggattaa
      721 ttgcagtgat gtttttctct ggatgtgcaa cttattacaa tcctgttacc ggaaaacagg
      781 aaaccacagc ttacactgaa caagatgaaa tagatatggg gattgctctt gataaaaaaa
      841 tttgttctga attcaaaatt gttgaaaacc ctgaaaaggc attcccggga ttcaccagta
      901 tttgttctcg tgttgccaga aacaatgaca gaaatcatct taactataca ttcagggtaa
      961 tagaaaataa ggaagtcaat gcgttttcca tccctggagg gtttgtttat gtttataccg
     1021 ggttgttgca gcaggtaaat tcgcctgatg aacttgcctg tatcgtaggg catgaaatcg
     1081 gtcacatctg taacagagac ggtgtccacc ggcttgaaaa acaaattctt tactcaattc
     1141 ctgcaagcat attgtttggt tcaggcagac agaaagccat acaacaggcg gcagatgctg
     1201 tattcaccct ttcaatgctg aaatacagtc gttcccagga aatagaagca gatactcttg
     1261 gaatgacata tgcctatcgt gcgggattca accctgaagg aatgatttcc tttttcaaaa
     1321 aaatggaaga acttgaaaag aatcagccat caattccact gactttttta cgagatcatc
     1381 cagaaacaaa agaaagaata aaaaatgcac aatcagttat caataatttg aaacagaata
     1441 aataagcagc taaacatgtt cgtcctgctg gtcgatcagt tgaacaagaa catctgcaca
     1501 tgactcctta tcaagagcaa tctctgtctg gtcaattctg atgataaaac ctggaaaggt
     1561 ttcctggaca taaatcatac aacctggaac aattccataa cccgatagtc tcggaaactt
     1621 ctttccgcgc aaacaaacaa ctttcactct ggtgcccctg ggaacatcgc ataaccttag
     1681 gacccccatc tgatgaaaaa aaccccggcc agcccttctc ttcaatctcc tgccgcaaca
     1741 caaacccctt ggaataggct tgccatcagg acactgctgg ggatgcccaa gaaaatcgca
     1801 taatgcttca tcaatattac cctttgcgtc atgttcaata ataccagcca tttcattaaa
     1861 ttcttccata cccagaaaat catgcaaaaa aacctcccat aacctgtgtc tcctcaccag
     1921 acactttgca atttcttcac cctgttttgt aagaaccgca ccacgctctg aaacttcaac
     1981 gagacccatt ttcctgagat gttccatctt ctctttgtca aatcccgtaa caaaagattc
     2041 tttcaattct cgctttttcc agatttcttc cagaatatct tcaagttctc tatccataca
     2101 ttctccttaa aataacccgg aagcgcttaa cacagcgcta agaaggaaac caacgacaaa
     2161 ggcgataacc atcgcaaaaa tggaaataag aattgccgca aacaatcccc gttcctttat
     2221 cattacaaaa aactgcgcaa tacagggcac aaaaagtgtt aatgttactg cacttaccac
     2281 agcctgctgc ggatttaaaa tccctttgct aaaaaggtca aacatgccgg cagcgccgta
     2341 gtcccttctt aaaaaaccca ttacgaatac cttgctggtt tccaaaggaa gacccattgc
     2401 tattacagga tacgaaatcc atgaaacgaa atatgaaaga acccctgctc tgtcaagaag
     2461 aaacagaatc attgttccaa gcacaaaaaa cggtaacgct tcttttaagt accatagaat
     2521 tcttcttcct gtttttagaa caatgtttct tatccctgga acccttaatg gaggtatttc
     2581 catggaaaag attgcatttc ctggaacaag aagattcaat gcgctaccaa caataatcat
     2641 cacaatcaca attgtaacca tccatgccaa aagagcaaaa ggcgaaatag atcccagcac
     2701 gccaagaaca actcccagtt gcgctgaaca gggtattgcc acagcaagta aaactgttgt
     2761 tatgattttt tcttttcgcg tatcaaggat tcttgttgtc agtgtagcca ttgttccaca
     2821 accaaaaccc agcacaagag gtaatactgc tttaccagta agccctatgg atttgaaaat
     2881 cctgtccata agaaaagcca ggcgtggcag atagccacaa tcctcaagaa aaccgaaaaa
     2941 caggaagaac gttccagtaa tgggcaaaac tattcctatg ccatatgtga gcgccatcgt
     3001 gataattcca aattctccga cgaaaaaatc ttttaccagt tggggtgttc tttcaaatat
     3061 gaattgaaaa aatggattaa taattctgcc aaacagtttt ccctcaatca aatcaacaag
     3121 taccactgcg ccaaattttc ccacaataag atacataaaa aataaaacca gtaatgcaat
     3181 caacactcca gatatcgggt gtatcgtaac ccgactcatg aactgctgaa acttcccgcc
     3241 tctgggcttg ctctttgttg caaccatgtc aataattttt tcagattctt taatctgcga
     3301 agtaacaata agataattga gcggctgtga cattgaagat tgaaaacgtt cccagatact
     3361 ttctataagt ttttcgttca catgtggaaa tttcttacag atatcgtgaa tactatcagt
     3421 ggcgccggca agcaaacttg caatgcccct gctatgaaag gaagtgtcag gtaaattttt
     3481 tttaatgttt gctatcatgt tctccacatc atcatgatac acaattttgt atttaccttt
     3541 ccctgctctg gttattgatt ctttcaactg atgtaatcct acatattcag ttgctatcat
     3601 cggaacaaca ggcactccaa gaatttcgga aagttttttc cagtcaataa aaattcccct
     3661 gtcatttgct tcgtccatca tattgagaac aacaacatgt ctcactccaa gttcacacaa
     3721 tgtaaaactc aaaaccaggc cccttttgag gttttttgcg tcaatgacaa gaacaacaat
     3781 tgcatcagga ttttcaagca atatttccct ggttacctgc tgatcctctg ttttaccaag
     3841 gaagtcgata attcctggcg tatcaatgac cacatagttt tcatttccaa ttttcgcttt
     3901 gccctggctg atttcaaccg ttgtgcccgg atagtttgaa actatagcat aactaccggt
     3961 aagaaggtta aaaatcacac ttttgcctac attggcatta ccaacaagaa taattttttt
     4021 t
//
LOCUS       DAORYU010000129         4019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_14989_length_4019_cov_4.256811, whole genome shotgun sequence.
ACCESSION   DAORYU010000129 DAORYU010000000
VERSION     DAORYU010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14989_length_4019_cov_4.256811"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..1526)
                     /locus_tag="PLW07_04930"
     CDS             complement(30..1526)
                     /locus_tag="PLW07_04930"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOL49902.1"
                     /translation="MIPDFSRDDISVVSHYLGKIFIAFAIAQLFPVFVGLICREFNPV
                     IDFFISIIACLAFGSILLYFSPSPQEINWRQGMTIVASSWFLAMCFSALPLYLSGHYE
                     CFLDACFESMSAYATTGLVLVKDLDHMSHAHNIWRHLMCFIGGQGIILVALVFFFRGG
                     DALRIYVGEARDDKILPNSITTARFIWMVSFTYLVICSSMLAIVLLFHGVPFMRSVFH
                     GLCIFMAGWDTAGFAPQSLNMLYYHSPVFELVTIIIILLGATSFGVHYAVWTGKFREL
                     REDFEVKILMFTIFFTYLIVLVGFSGYTYQFVDSFSFFRTTFYQIISAHTGAGYTNTY
                     SSQLAQWPELALFGVILAMGLGGCMNSTAGGIKVFRIGLFYKSVKREIRRLAYPESSY
                     IKEFFHHQREISLDDTMVKTAMILTICYILLYLSGAIIASFFGYSFIHGLFESVSAAA
                     NVGLSVGITGPSMPVVLKFWYILEMWAGRLEFFAIFILVRFLVKSVKR"
     gene            complement(1523..1669)
                     /locus_tag="PLW07_04935"
     CDS             complement(1523..1669)
                     /locus_tag="PLW07_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49903.1"
                     /translation="MKIRLMRYLNMLKKGIIGISIELVYPLIVTISAFVLSFIMFISE
                     IFNR"
     gene            complement(1671..2330)
                     /locus_tag="PLW07_04940"
     CDS             complement(1671..2330)
                     /locus_tag="PLW07_04940"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HOL49904.1"
                     /translation="MRIIIVGGGKVGYFLAERLARTHYVSLIEKDKQITNELAGNRNI
                     LIINGDGCQTDILVQAGVKDADVVAAVTGKDEDNLVICQIAKDIFKVKRTVARVNDPK
                     NEGIFFHLGVDIAIDSTAIIAKIIEEEVSLEDMINLCAFKKGQLSLVRIDLPEDAPIV
                     KKSLKEIELPAEAVIVAVLRKEHLIVPDRDFVFEQGDEVVALTKVEDEGEILNALIGR
                     V"
     gene            complement(2327..2971)
                     /locus_tag="PLW07_04945"
     CDS             complement(2327..2971)
                     /locus_tag="PLW07_04945"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOL49905.1"
                     /translation="MYFIVIGCGKVGSQLARFLSESHNVVVIDRDPASFQVLQPVFNG
                     ITIVGNCINLDVLKDAGIEKCDGIAAVTSDDNVNIVVGQIARKMFHINKCIIRIVDPV
                     KNEIYRKLGYDVVSGQRLIAAIIRDKLIEKGTTTYIVESGEITFLEILINGKKSGTRV
                     SDLNIPEEFKVVVVVKQDGPVIPDDGYVVKEGDLIIGVARMKSMQRIKKSLEIT"
     gene            complement(3018..3947)
                     /gene="thrB"
                     /locus_tag="PLW07_04950"
     CDS             complement(3018..3947)
                     /gene="thrB"
                     /locus_tag="PLW07_04950"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HOL49906.1"
                     /translation="MKTDFFLTIPMKKIKKISISVPGCIGNVGSGFDCMGISIKLFNK
                     FIFERSEKFSISVSSSDVDSTRNNLCYKSFEKVCRAFRKKVPVVKLNIDNKIPAGKGL
                     GSSGTAVLAGIIAGLIFSGEKVTLRKVLKAGKEIEKHLDDIAASFLGGLVVVAEHNGE
                     IVCKQFEIPSDISAVFFITEKAFPTDTARKILPEKVPLSDAIFNVSRSALVPVAFSEK
                     NSELLGIALQDRLHQIYRKKVYPHFKILYDSAMRSGAAGCCLSGAGPSVVAFCFGDTE
                     KLVKDWTEVVRKNKIAGQIRILEPGGQTTWGIY"
BASE COUNT         1302 a          931 c          624 g         1162 t
ORIGIN      
        1 caaaatggta aaaacaatcc ctgcagtttt catcttttca cactttttac gagaaatctt
       61 acaagaatga atatcgcaaa aaattcaagt cttccagccc acatctcaag tatataccag
      121 aatttcaaca ctacaggcat cgaaggacct gtaataccca cagaaagacc aacatttgct
      181 gcagcagaaa cagattcaaa aagtccatga ataaagctat atccaaaaaa actcgcaatg
      241 attgctcctg aaagataaag cagaatataa catattgtaa gaatcattgc tgtcttcacc
      301 attgtgtcat ccagagaaat ctctctttga tgatgaaaaa attcttttat atatgaactt
      361 tcaggatagg caagccggcg gatttctctt ttcactgatt tataaaacaa ccctattctg
      421 aaaaccttta ttccgccagc agtggaattc atacaaccac ccagccccat tgccagaata
      481 actccaaaaa gtgccagttc tggccattgc gcaagctggg atgaataagt attggtatat
      541 cccgcacctg tatgggcaga aataatctga tagaatgtcg ttctaaaaaa tgaaaatgaa
      601 tcaacaaatt gatatgtata tcctgaaaat ccgacaagaa caatcaggta ggtaaaaaag
      661 attgtaaaca tcagtatttt tacttcaaaa tcctctctca attccctgaa ctttcctgtc
      721 cagacagcgt aatgtacacc aaaacttgtt gctccaagaa gaatgataat aattgtcaca
      781 agttcaaata ccggactgtg gtagtaaagc atgttaagtg attgcggagc aaaaccagca
      841 gtatcccagc cagccataaa aatgcacagt ccgtgaaaaa cagacctcat aaaaggaact
      901 ccatgaaaca atagcacaat agcaagcata cttgaacaga taaccagata tgtaaaactc
      961 accatccaga taaatcttgc tgtcgtgata ctattgggaa gaattttatc gtccctcgct
     1021 tctccgacat agattctgag agcatcccct cccctgaaaa agaaaacaag tgcaacaaga
     1081 ataatgccct gcccaccaat gaaacacatc agatgtctcc agatattgtg agcatgagac
     1141 atatgatcaa gatccttcac aagtaccagt cctgttgttg cataagcgct cattgattca
     1201 aagcaggcat caagaaaaca ttcgtaatga ccactcaggt agagaggaag ggcagaaaag
     1261 cacatcgcca gaaaccagct ggaagccaca attgtcattc cctgtcgcca gttgatttcc
     1321 tgcggagaag gcgaaaaata aagaagaata gaaccaaatg caagacatgc aatgattgat
     1381 ataaaaaaat ctattacagg attaaactct ctgcatatca gtccaacaaa tacagggaaa
     1441 agttgcgcaa tagcaaaagc aataaaaatt ttccctaaat aatgactgac aacagaaata
     1501 tcatccctgc taaaatcagg aatcatctgt tgaaaatctc cgaaataaac atgatgaaac
     1561 tgagaacaaa agcagaaatg gtaacaatca acggatatac aagttcaatg gatataccaa
     1621 taatcccttt tttgagcata ttgagatacc gcattaatct tattttcatg tcacaccctg
     1681 cctattaaag cgttcagaat ctcaccctca tcttcaacct ttgtcaaagc gacgacttca
     1741 tctccctgtt caaaaacaaa gtccctgtca ggcactatga gatgttcctt tcttaacaca
     1801 gccacaatga ctgcctctgc tggaagttca atttccttca gacttttttt cactattggc
     1861 gcatcttccg gtaaatcaat tctgacaaga gacaactgcc cttttttgaa agcgcagaga
     1921 tttatcatgt cctcaagaga aacctcttct tctataattt ttgcgatgat tgctgtactg
     1981 tcaatagcga tatccacgcc cagatgaaaa aatatccctt catttttagg gtcattgacc
     2041 ctcgcaactg tccttttaac cttaaaaatg tcttttgcaa tctggcatat cacaagatta
     2101 tcctcatcct tgccagtgac agcagcaaca acatctgcat cctttacccc tgcctgtaca
     2161 agaatatctg tctggcaccc atccccattg atgataagaa tatttctgtt tccagccagt
     2221 tcattggtaa tttgtttatc cttctcaatc aaggaaacat aatgcgtcct tgccagtctt
     2281 tcagcaagaa aataaccgac ttttcctcca ccgacaataa tgattctcat gtgatctcca
     2341 gactcttttt gattctttgc atggacttca ttcgcgcaac tcctataata agatctcctt
     2401 ccttcacaac gtatccatca tcaggaatta caggtccatc ctgtttgacc acgacaacaa
     2461 ccttaaattc ttcaggaata ttcaaatcag aaacccgtgt gccagatttt ttcccattga
     2521 ttagaatttc aagaaaagtg atttctccgc tttcaacaat atatgttgtt gtaccctttt
     2581 caataagttt atcccttatg attgcggcaa tcaatctctg tccgctaacc acatcatagc
     2641 caagttttct gtatatttcg ttcttcacag gatccacaat tctaatgata cacttgttaa
     2701 tatgaaacat cttccttgct atttgaccaa caacaatgtt gacattatca tcactcgtaa
     2761 cagccgcgat gccatcacac ttttcaattc cagcatcctt aagaacatca aggttaatac
     2821 agtttccaac gatggtaata ccattaaaaa ccggttgcaa cacctgaaaa cttgcagggt
     2881 ccctgtcaat caccacaaca ttgtgactct ccgatagaaa cctcgcaagc tgacttccaa
     2941 ccttcccgca tccaatcaca ataaaataca tagcacttta ctcctctctg tctcgtattt
     3001 taatacaaaa atgcctgtca ataaataccc catgttgttt gtccaccagg ttcaagtatt
     3061 cttatctgcc ccgcaatttt attttttcga acaacttctg tccaatcttt taccagtttt
     3121 tcagtatcgc caaaacagaa agcaaccact gatggtcctg caccacttaa acaacaaccg
     3181 gcagctccgg atctcattgc agaatcatat aaaattttga aatggggata aacctttttt
     3241 ctgtatatct gatgcagtct atcctgcaat gcaataccaa gcagttcaga gtttttctca
     3301 ctgaaagcaa cgggtacaag agcactcctt gatacattga agattgcgtc tgaaagtggg
     3361 actttttctg gaagaatttt tcttgccgtg tctgttggga atgccttttc tgtaatgaaa
     3421 aatacagccg aaatgtccga tgggatctcg aattgcttgc agacaatttc tccattgtgt
     3481 tctgcaacaa ccaccagtcc tccaagaaaa cttgcggcaa tatcgtcaag gtgtttttca
     3541 atttctttgc ctgcttttaa aacctttctg agagtcactt tttcaccgga aaaaattaaa
     3601 cccgcgataa ttcctgcaag aaccgcagtg ccactgctac ccaatccttt gccagcagga
     3661 attttgttat ctatgttcaa tttcacaaca gggacttttt ttctaaatgc cctgcagact
     3721 ttttcaaaag atttataaca taaattgttt ctggtactat ccacgtctga agacgaaaca
     3781 gagatggaaa atttttctga cctttcaaag ataaatttgt taaacagttt tatgctgata
     3841 cccatacagt caaaaccact accaacattt cctatgcaac ccggcactga aatagaaatt
     3901 ttttttattt ttttcatcgg tattgtgaga aaaaaatcag tcttcaacac gaatgacctg
     3961 cgttttttcc tttatgcagg tcattctgtt aattgcagat actgcctttt tgatatttt
//
LOCUS       DAORYU010000130         3945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_15377_length_3945_cov_3.506170, whole genome shotgun sequence.
ACCESSION   DAORYU010000130 DAORYU010000000
VERSION     DAORYU010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15377_length_3945_cov_3.506170"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..609)
                     /gene="ispF"
                     /locus_tag="PLW07_04955"
     CDS             complement(136..609)
                     /gene="ispF"
                     /locus_tag="PLW07_04955"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOL49907.1"
                     /translation="MRTGLGFDVHRLEPDRKLILGGIEIPYHLGLKGHSDGDVILHAI
                     SDAIAGALAEDDIGTTFPDTDESISGIKSDRILDHYYKILVLRKARILNIDIVIVAEN
                     PKLKKWYELMKKNIATLLDIDISQIGIKAKTMEGLGEIGQGKAIACLASILIDMR"
     gene            688..1677
                     /locus_tag="PLW07_04960"
     CDS             688..1677
                     /locus_tag="PLW07_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49908.1"
                     /translation="MEKKAIFQSVSLKWKAFDRLDENNLINLTRLGYKKHHEFDVPEG
                     VWQVFCLEKKIEEDILSQKKEEIVLFFAIFISKLHERKFPVSGICDNPDEVITGLKYL
                     SSFVFTDNDRKFLSSKLTIENAQDYGYIRGLIAGIILMLIDIIPWHIGNFRPQGVLST
                     FLEYTRIVYYGTPGFAIVVGMAAIGIYFTVLFILLPILAGNFFLFKARKKQQKQISIM
                     PECLADFEYGKDAESSLNEQHLSRVNNLKRDEIYRRVSTLWQNFKREDFYELYDIFRD
                     GLLAPEVIYNLLEKITRNCPDFDFKKFLKIMIDIMKRDTEVVIKVSDKSQEKT"
     gene            1674..2909
                     /locus_tag="PLW07_04965"
     CDS             1674..2909
                     /locus_tag="PLW07_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49909.1"
                     /translation="MNRSIQKNLIVFFMVFILTAAGLTREQKQQYVECEMFPIPESTH
                     NGSGYTAIEVGKDGNIYVGTAYYGSSAHLVRFNPRKLKWDDIIDAHKITRETGTGLDS
                     QSKFHAKILVDADGTIWAATKQGNENFIERPEFGENPSGYPGGHVFSFNPKTNQVIDH
                     GILRKQEGIMGGSIDKKRKKLYYWSDPKQHFLIYDIETGSLKDLGTTGGSPRYTAIDW
                     KGRVFGTGRPGIIWMYDPETDRLYDLIVKIEGPGQYKDPYAMVASSDGKKIFACAIGG
                     EYVMEFDLSTICFTSASEDADGFIICRHVGKSYPENEKKQGDQHTGVLGKDGCFYFPN
                     VIDKTPYLMRYNPEKVRVECLGIIRIKGNPDLKPLYAQGACTTTDGTIYMKFISAGAG
                     KLMPYSIVRFNKLSTKPGI"
     gene            2919..3884
                     /locus_tag="PLW07_04970"
     CDS             2919..3884
                     /locus_tag="PLW07_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49910.1"
                     /translation="MRKISFIVFSILLITFSSIFCEEIINFALDILNLPFPPYQRNVV
                     KLIEAPNGLIYGITSAPGNSSGRIFVIDKNYKTHEINRLPDDVLQVDSVIFNSDITWT
                     WDKTRRKIIALDKSGTLKVYDKNGPGKEILKIAGTRPYEPQGFQISRAFVQDTTGNLY
                     TAGKDGIMFKFNPETLEIKKLQAKLPAIKGREPWASLDAATVGNDGTIYLGTFDGYIA
                     SFNPETGTLINLGKPLRQQRIQSLVFFNGIVFGIGGESDGLARWFIYDPLTRGFNLGG
                     TLKSKENKLIYEPVNAMIATNEGVIIGSFSGRLGSLFIIKTGKEK"
BASE COUNT         1267 a          721 c          790 g         1167 t
ORIGIN      
        1 ctttctgaat atcttttgat tcccgaccag tttaaagaga ttctaaaaag agcgacacac
       61 gatgatcctg ccatgcgata ccagacagcc gaggaatttg cttttgcact ttcaagattt
      121 atcgaacata ctgattcatc tcatatcaat gagaattgat gccagacatg ctatggcttt
      181 tccctgacca atttctccaa gaccttccat cgtttttgct ttgatgccta tctgagaaat
      241 atcaatatca agaagggtcg caatattctt cttcatcagt tcataccatt tcttcaactt
      301 aggattttct gcaactatta caatgtcaat gttcaaaatc cttgcttttc tcaaaacaag
      361 aattttgtaa tagtggtcaa gtatcctgtc gctttttatt ccacttatag attcgtcagt
      421 atcaggaaag gttgttccga tatcatcctc tgcaagggct cctgctattg cgtcgctgat
      481 ggcatgcaaa atcacgtctc cgtcactgtg acctttcaac ccaaggtgat atggaatttc
      541 tattccccca aggattaatt tcctatccgg ttccaacctg tgtacatcaa aacccaatcc
      601 tgtgcgcata tatgcaaata taccagaaaa ttttcattgg tcaagtatcc aatttccgta
      661 atttcaattt cgttataaaa tagttaagtg gaaaaaaaag cgatttttca atcagtgagt
      721 ttaaaatgga aggcattcga taggcttgat gaaaacaacc tgatcaatct tacaagatta
      781 ggatacaaaa agcaccacga atttgatgtg ccagagggcg tatggcaggt attttgtctt
      841 gagaagaaaa tagaagaaga tattctgtca cagaaaaaag aagaaatagt cttgtttttt
      901 gcgattttta tttcaaaatt gcatgaaaga aaatttcctg tttccggaat atgcgacaat
      961 ccggacgaag tcataactgg attaaaatac ctttcatctt tcgtattcac tgataatgat
     1021 agaaaatttc tgtccagtaa attaacaatt gaaaatgccc aggattacgg ttacatcagg
     1081 gggctcattg ctggaataat cctgatgttg attgacatta taccctggca catcggtaat
     1141 ttcagaccgc aaggtgtact ttccactttt ctcgagtata caagaattgt gtactacgga
     1201 acgcccggtt ttgccattgt tgttggaatg gctgccatcg gaatttactt tactgttctt
     1261 tttattctgt tgcctatact ggctggcaat tttttccttt ttaaggcaag gaagaaacaa
     1321 caaaaacaaa tttccataat gcccgaatgt cttgctgact ttgaatatgg taaggatgca
     1381 gaatcatcct taaatgaaca gcatttgtca cgtgtcaaca atctgaagag agatgaaatt
     1441 tacagaaggg tatccaccct gtggcagaat tttaagagag aggactttta tgaactgtac
     1501 gatatcttta gagacgggct gttagcgccc gaagtaattt acaatctgct tgaaaaaatt
     1561 accagaaatt gcccggattt tgattttaaa aaatttctta agattatgat agatatcatg
     1621 aagagggaca cagaagttgt aatcaaggtt tcagataaaa gtcaggagaa aacatgaaca
     1681 ggtccattca aaaaaacctg atcgtatttt tcatggtttt tatccttacc gccgcaggtt
     1741 taacccggga acaaaaacaa caatatgttg aatgcgagat gttcccgatt ccggaatcaa
     1801 cacataatgg aagtggttat acagcaattg aagtgggaaa ggatggcaat atttatgttg
     1861 gcactgcata ttatggttca agcgcccatc tggtcagatt caatccccga aaattaaaat
     1921 gggacgatat cattgatgct cacaaaatta caagggaaac tggaacaggt cttgattcac
     1981 aaagtaaatt ccatgcaaaa attcttgttg atgcagatgg gactatatgg gcagcaacaa
     2041 aacagggaaa tgaaaatttc atagaaaggc ctgaattcgg agaaaatccc tcagggtacc
     2101 cgggtggaca cgttttttca tttaatccaa aaacaaacca ggtgatcgac cacggaattc
     2161 tacgtaaaca ggaaggcatt atgggaggtt ctattgataa aaagaggaag aaattgtatt
     2221 actggagcga tccaaaacag cacttcctta tttacgatat tgaaaccggt tcattgaaag
     2281 accttggaac caccggagga tctccaaggt acactgcaat agattggaag ggcagggtgt
     2341 ttggtacagg caggcccggt attatatgga tgtatgatcc tgaaactgat agattatatg
     2401 acctcattgt aaaaattgag ggccccggtc aatacaagga cccttacgca atggtggctt
     2461 cttctgatgg caaaaaaatt ttcgcgtgtg cgataggtgg cgagtatgtg atggagtttg
     2521 acctgtcaac aatttgtttt acatctgcat cagaagatgc tgatggtttt attatttgca
     2581 ggcatgtcgg gaaatcgtat cctgaaaacg aaaagaaaca aggagatcag catactggtg
     2641 ttctgggcaa ggatggttgt ttttactttc cgaatgtaat tgacaaaaca ccgtatctga
     2701 tgagatacaa tcctgaaaaa gtaagggtag aatgtcttgg aattatcaga ataaaaggaa
     2761 atccagacct taaaccactt tatgcgcagg gcgcctgtac cactaccgat ggcacaattt
     2821 atatgaaatt tatctctgca ggcgcaggaa aactgatgcc ctattcaatt gtgaggttca
     2881 ataaattgag tacaaaacca ggtatttgag tggttaatat gagaaaaata tcttttatcg
     2941 ttttttccat cctcctgatt acgttttctt ctattttctg tgaagaaata ataaatttcg
     3001 cacttgatat actcaatctt ccctttcctc cttaccagag gaatgttgtc aaactgattg
     3061 aagcaccaaa cggtttgatt tatggaatta catccgcacc aggaaattca tctggtagaa
     3121 tcttcgttat tgataaaaat tataaaacac atgaaattaa tcggcttcct gacgatgttc
     3181 ttcaggtaga cagtgtcatt tttaactctg atattacctg gacatgggat aaaacccgca
     3241 ggaaaattat cgccctggat aaatctggaa ctttgaaggt ttatgacaaa aatggacctg
     3301 gtaaagaaat tttgaagatt gcaggtacca gaccgtatga accccagggt tttcaaattt
     3361 cacgagcatt tgtacaggac acaacgggta atttatatac tgctggaaaa gacggcatca
     3421 tgttcaaatt taatcccgaa acactggaaa ttaagaaatt acaggcaaaa cttccagcga
     3481 taaagggaag agaaccatgg gcaagtcttg atgctgctac agtcggaaac gatggtacaa
     3541 tctatcttgg aacttttgat ggttacattg cttcctttaa tcctgaaaca ggtacactga
     3601 ttaaccttgg aaaaccactg agacagcagc gcattcagtc gcttgttttt ttcaatggca
     3661 tcgtttttgg aataggtggg gaatctgatg gtcttgcccg ctggttcatc tatgaccctt
     3721 taacaagagg ttttaatctt ggaggaacat tgaaaagcaa agaaaataaa ttaatatacg
     3781 aaccagttaa cgcaatgatt gctacaaacg aaggagttat tattggaagt ttttcaggaa
     3841 gattgggctc tctcttcatt atcaaaacag gaaaggaaaa atgaaaaaat atcttttaat
     3901 tttcatgtta accataatta tcacgggtac atgcttccaa acagg
//
LOCUS       DAORYU010000131         3938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_15407_length_3938_cov_3.838269, whole genome shotgun sequence.
ACCESSION   DAORYU010000131 DAORYU010000000
VERSION     DAORYU010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15407_length_3938_cov_3.838269"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="PLW07_04975"
     CDS             <1..270
                     /locus_tag="PLW07_04975"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOL49911.1"
                     /translation="GSYTALKEMGVTVPYEVAIMGYGNLEMGRFLEVPLSTVEQSAEE
                     MGKTGATLLVDRMRWKRKTDDIQEIVVPTKLVIRDSCGINKKIKI"
     gene            306..1082
                     /locus_tag="PLW07_04980"
     CDS             306..1082
                     /locus_tag="PLW07_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOL49912.1"
                     /translation="MTKRGNIFGQDAFTLIELLVVIAIIAILGAMLLPALSKARAKAR
                     QAVCAANMKQMMLCWRLYIDDYSGYLPACDPNFVWGYPQRTTWVHLIIPYSSTKYRLY
                     IPDANNVYMIIQSKGSVFICPSVAKYYTPGVSYVTSVSYGMNMYGVGGRDTVGLGYAL
                     PSDKYRPGMKKEAQIKKPDATLVFVDSFDKINLRNFDVDPSKLNDPSLSKVDLRHSGF
                     ANVAFADGHVESMNYGTLCAPYRTPAYPSIITSYLWGWGY"
     gene            1120..>3938
                     /locus_tag="PLW07_04985"
     CDS             1120..>3938
                     /locus_tag="PLW07_04985"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOL49913.1"
                     /translation="MNNRGTSISIGCLLLFLIKATIPLYGGQDLVKVNFKNLFAKKSW
                     PMVSNGFEDASVVVDTEIFFTSPPAMKIDSGKSNQKVVSLTFYLIKEQFQLFKGKKVK
                     FTAKVKRITGQEILYIQVRFNKLDDEGKYQFLFGKSQPITLSDSKWTDVEFNIDVPNQ
                     QDVNAMNFQIYLTKSTVPTVIVVDECEVSERNPESTLNDGKKQNSIPLLFSLPQQDEQ
                     PLEIVKEGKPAATIIIAKIPTQVVSYAAKELNEHIRLSTGTELPVSTDEQPVSGPAIH
                     IGTTTLSVQLGISPDLLPPDTWVIKRFKNNIIISGGDNNRNINPVGKDLVPFGTLYAT
                     YEFLERTIGVRWFWPGDDGRLVPKHRSIVIDKINWQGSPSYATRYVFYGIPEGITPEE
                     AWTWWRRMRLGGIDGNPFGMHSFTDWNKKYGKEHPEWFALQFNGQRLNLEDQDEHKNG
                     HLCYSNPDVLNKVVAEIKSNLKKQPHLKYFSVMPGDSNERYYCQCKDCQSLLKVDQPS
                     KKYSFAVWSFVNKVADEVRKSFPAVFIKCCAYDGYRNPPEDVYFQPNVTVTYCVPEEL
                     RNPWTDDFKKKYVSEITAWNKKVSDLYVWDYWLYRWKPGLYGAPAIFPHLLQEIYLLE
                     QGRVRGHVIELCNKDVSGVSHGFWQDWMMDQINVYVGFKLLWNINQDVDEILDDYYKF
                     FGPAEPLIRSFYESMEKAFLDTSTKNKDNTWDWETCWYKTYTPEFINRTMKYLKDAEQ
                     MTRDKEPYHKRVKKVLQEFAAFEMAGKNFSPSRKESKNKQIIVPYSKQIPIIDGKLNE
                     NLWNQTLRLNDFVDLFNNPASVQTEFFIAHDGKNFYIGIRSYLKNKEIRKLSASCSID
                     NPLWEAESCEFFLSPDSVQCYQFLIGPDNFFTDIYTKDIENFKIDDINWNAKDIEYAT
                     YVSDEFWSAEIKIPFSSIQFNPESE"
BASE COUNT         1319 a          697 c          812 g         1110 t
ORIGIN      
        1 ggttcgtaca ctgcattaaa agaaatgggt gtaactgtgc cttatgaagt cgccattatg
       61 gggtatggaa atcttgaaat gggcaggttt cttgaagttc cattgagcac agttgagcag
      121 tcagcagagg aaatgggtaa aacaggggca acacttcttg ttgacaggat gagatggaaa
      181 agaaaaacag atgatattca ggagatagta gtaccgacaa aactcgtgat acgggattcc
      241 tgcggtataa acaaaaaaat aaaaatatga gaaccctttt tatcaggaga agggaggtga
      301 aaaagatgac taagcgaggt aatattttcg gtcaggatgc tttcacgttg attgaacttc
      361 ttgtggtaat agcaataatc gctattcttg gtgcgatgct attaccagca ctttcaaagg
      421 caagggcaaa agcaaggcag gccgtttgtg ctgcaaatat gaagcaaatg atgttatgct
      481 ggcgattgta tatagatgat tattcaggtt atttaccagc gtgtgatcca aattttgttt
      541 ggggatatcc tcaaagaact acatgggttc atctgataat tccttattcc agcacaaaat
      601 accgattgta cattcctgat gccaataatg tttatatgat tatacagagc aagggaagtg
      661 tttttatctg cccatcagta gcaaaatatt atacacccgg tgtctcctat gttacctccg
      721 tttcatacgg gatgaacatg tatggggtcg gaggtcgtga cacggttgga cttggttatg
      781 ctttgccatc agacaaatac agaccgggaa tgaaaaaaga agcacagata aaaaaacctg
      841 atgcaacgct ggtttttgtt gatagttttg ataaaataaa tctaaggaat tttgatgtag
      901 acccatcaaa acttaatgat cccagtttat caaaagtcga tttaagacat tccggttttg
      961 cgaatgtggc ttttgctgat ggacatgtag aatccatgaa ctatggaact ctttgcgcac
     1021 cgtacagaac acctgcttat ccatcaatca tcaccagtta tttgtggggg tggggatact
     1081 aatattaatt tttactccat agtaatgaag ggattgaata tgaataacag aggaacgtct
     1141 atatccatag gttgtttatt attattttta ataaaagcca caatacctct ttacggagga
     1201 caggatcttg tcaaggtcaa ttttaaaaac ctttttgcaa agaaatcgtg gcctatggtt
     1261 agcaacggat ttgaagacgc ttctgttgtt gtggatacag aaattttttt tacgagtcca
     1321 ccggcgatga aaatagattc tggaaaaagc aatcaaaagg ttgtttccct gacattttat
     1381 cttataaaag agcagtttca gttatttaaa ggtaaaaagg tgaaattcac ggcaaaggtt
     1441 aaaagaatta cagggcaaga aatactatac atccaggtga gatttaataa actggatgac
     1501 gaaggaaagt atcagttctt attcggaaaa tcacaaccca ttacgctttc tgactcgaaa
     1561 tggacagatg tggaatttaa tattgacgta cccaaccagc aagacgttaa tgccatgaat
     1621 ttccagatat atctgacaaa aagtacagtt ccaactgtga tcgttgtgga cgagtgcgag
     1681 gtgagtgaaa gaaaccctga atccacatta aatgatggga aaaaacagaa tagtatacct
     1741 cttctatttt ccttaccgca gcaggatgaa caaccacttg aaatagtaaa agagggaaaa
     1801 cctgccgcaa ccatcattat cgcgaaaatt ccaacgcaag ttgttagtta tgcggccaaa
     1861 gaattaaatg aacatataag attgagcaca ggcacagaac ttcctgtctc cacagacgaa
     1921 caacccgttt caggaccagc aatccatatt ggaaccacga ccctttcagt gcagcttggg
     1981 atttctcctg acttactacc acctgatacc tgggtcatca aaagattcaa aaacaacatc
     2041 attatttctg gtggtgacaa taaccgtaat attaatcctg ttggaaaaga ccttgtacca
     2101 tttggtacgc tttacgcgac atatgaattt ctcgaaagaa ctataggtgt tcgctggttc
     2161 tggccaggcg atgacgggcg cctggttcca aaacacagaa gtattgttat tgataagata
     2221 aactggcagg gttcaccttc ttacgctacc cgttatgttt tttatggaat accagaaggt
     2281 attacacccg aggaagcatg gacctggtgg agaagaatgc gacttggtgg tattgacggg
     2341 aatccatttg gaatgcactc atttactgac tggaataaaa aatatggcaa agaacatcca
     2401 gaatggtttg cccttcagtt taacggacag agattaaacc tggaagacca ggacgaacac
     2461 aagaacggcc atctatgtta cagtaaccct gatgttctca acaaggttgt tgctgaaata
     2521 aaatctaact tgaaaaaaca acctcacctt aaatattttt ccgtgatgcc aggcgatagt
     2581 aatgagcgtt actactgtca atgtaaagat tgtcaaagtt tgttgaaagt tgaccagcca
     2641 agtaaaaaat acagttttgc agtatggtcc tttgtaaaca aggtagcaga tgaagtgcga
     2701 aaatcatttc cggctgtatt cattaaatgc tgtgcctatg atggatatag aaatccacct
     2761 gaagatgtat actttcaacc aaatgtcact gttacttact gcgtacccga agaattgaga
     2821 aatccctgga cggatgattt taaaaaaaaa tatgtgtcag aaattacagc atggaataag
     2881 aaagtgtcag atctctatgt atgggactac tggttatacc gctggaaacc aggtctttac
     2941 ggagcgcctg caatctttcc acatcttctt caggaaatat acctacttga acagggacgt
     3001 gtacgcggac atgtcattga actatgcaat aaagatgtat caggagtttc acatggattc
     3061 tggcaggact ggatgatgga ccagatcaat gtttatgtag gattcaaact gttgtggaac
     3121 attaatcaag atgttgacga aattcttgat gattattaca agttttttgg acccgcggag
     3181 cctttgattc gcagttttta cgaaagtatg gaaaaagcct tccttgatac ttccacaaaa
     3241 aacaaagata atacgtggga ctgggaaaca tgctggtaca aaacatatac tcccgaattc
     3301 attaaccgga caatgaaata tctcaaagat gccgaacaga tgacacgtga caaagaacca
     3361 tatcataaaa gagtgaaaaa agttctacag gaatttgctg cctttgaaat ggcaggtaaa
     3421 aatttttcac catccagaaa agaatctaaa aacaaacaga ttattgttcc atattcaaaa
     3481 cagataccga ttattgatgg aaaactcaac gaaaatctgt ggaatcaaac actcagatta
     3541 aatgattttg tagacctttt taataatcct gcatcagtac aaaccgaatt tttcatcgct
     3601 catgacggga aaaattttta catcggcata cgttcttatt taaaaaacaa agaaattagg
     3661 aaactttctg cttcatgcag cattgataat cctttgtggg aagcagaaag ttgtgaattc
     3721 ttcctatctc ctgattcggt tcaatgttat cagtttttga ttggtcctga taattttttt
     3781 accgacatat atacaaaaga cattgaaaat tttaaaattg acgatatcaa ctggaacgca
     3841 aaagatatag agtatgctac ctatgtaagt gatgaattct ggtcagcaga aataaaaata
     3901 ccattttcat cgatacaatt taatccggaa tctgaatg
//
LOCUS       DAORYU010000132         3911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_15523_length_3911_cov_4.086618, whole genome shotgun sequence.
ACCESSION   DAORYU010000132 DAORYU010000000
VERSION     DAORYU010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15523_length_3911_cov_4.086618"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="PLW07_04990"
     CDS             complement(<1..821)
                     /locus_tag="PLW07_04990"
                     /inference="COORDINATES: protein motif:HMM:NF024882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HOL49914.1"
                     /translation="MERNFSRFVGIILLGVTIFLFLCLVTYSPDDPCFQFCQLGYPVK
                     DVPANFGGNFGANMSALMFFLFGWVAYLCVIAVFLYAWSKSWKRENFTVLEGASCIIF
                     IFVITGLVESIKRGFGTSGSSVFPAAGITGLLIGHGVVSYFGDKGGFFLLAITSAGLL
                     VASSRIFYAPFENIVSVFSNMWRPESVFVMKTTVKQKTKKVQPAKIPDSMEKATDETQ
                     TQRTIIPEPKEPPVTEIITIQPQEKSVLKKQATVKSGTYQLPSLSLLKVAKYPEK"
     gene            870..1235
                     /gene="rbfA"
                     /locus_tag="PLW07_04995"
     CDS             870..1235
                     /gene="rbfA"
                     /locus_tag="PLW07_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOL49915.1"
                     /translation="MSFENQNRRNLKFASTLKHQINEILQKEISDPRIGFVTITNIRV
                     SADRRHVTVYVSTLGNEEKQTESLKGLANASGYIRHLLSKKLETRFTPEVEFVKDENP
                     GNRIEEILKEIEQDKGKEQ"
     gene            1232..2851
                     /gene="argS"
                     /locus_tag="PLW07_05000"
     CDS             1232..2851
                     /gene="argS"
                     /locus_tag="PLW07_05000"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOL49916.1"
                     /translation="MTSSDIKEKLIKILTDAGLHFEQFSVFPAAKKEFGDFSTNLFFK
                     LKETANRVSIKDLISLVMQDTWMTKHFEKIEEKNGFLNFFLKDTLIYGVIDEILTKKQ
                     TFGKSGCGNNTKVLVEFVSANPTGPLTIAHGRQAAFGESLARILAFSGWKVTREYYLN
                     DCGRQMELLGISLKCRYLQMKGISAEIPEEGYLGEYLKDIAEKLPDGCENKDASFFQE
                     IAKQEILNMIRQDLDQFNVRFDNYFSEKQLRDSGKVEKIIQQLKENSLVYENEGALWF
                     KSTIYGDDKDRVLIKSDGSYTYLAPDIAYHEDKIKRGYHLLINLWGPDHAGYIPRIKA
                     AVAALGFFPEKLQIIIVQLTTLYRGKVKISMSTRKGQFITLRQLMDEVGPDATKFFFL
                     FRKADSHLDFDLELAKKKTEENPVYYIQYAYVRAMHIIAFGIEKGFKENDILRADFSL
                     LKESQEIEILKKLKAFPVVVEYAARTLEISRIAHYILDLAGLFHSYYQKFRVVDENKK
                     LSCARLGLVQCVHTVMQNGLDLLGISKPEKM"
     gene            2903..>3911
                     /locus_tag="PLW07_05005"
     CDS             2903..>3911
                     /locus_tag="PLW07_05005"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOL49917.1"
                     /translation="MPTKKEITTIIQNIGLWNKQAIEKALQEQETTDRPLKEIFQNMG
                     LLPFGDVPATLFFQLGIVQQSLPLREIPPDVISLISPSFAKKHRVIPWEIREDQKLVM
                     VTDDPINIIASDYFKKNLNVPYEIEIIVTFPNEIDQLIAKYFEQQDIEDLSGMLQEVG
                     SADTIETLEDEEITTDEDEEALALQAPVVKIVNLIFVEALRRRASDIHLEPLEKKFRV
                     RFRIDGVLYEVVSPPKRVERAVIARVKLMSKMDLAEKRLPQDGRIMLDIGGRPIDFRV
                     STLPGIYGESVVLRILDKTKALKDLEQLGFMEQDRKTWENLLKYSGGILLVTGPTGSG
                     KT"
BASE COUNT         1349 a          736 c          780 g         1046 t
ORIGIN      
        1 tccttctcag gatattttgc tactttaagc aacgaaagag atggtaattg atatgtcccg
       61 gattttacag ttgcctgctt ttttaaaaca gatttttcct gtggttgaat tgtaataatt
      121 tcagttacag gtggttcttt tggctctgga atgatggttc tttgagtttg tgtctcatcc
      181 gttgcctttt ccattgagtc aggaattttc gcgggctgta ctttcttcgt cttttgtttc
      241 acggttgttt tcattacaaa aactgactca ggtctccaca tgtttgaaaa aacagaaact
      301 atgttttcaa aaggagcgta gaaaattctt gatgaggcaa caagtaatcc tgcactggtt
      361 atcgcaagaa ggaaaaatcc tcccttatca ccaaaataag acaccactcc atgtcctatc
      421 aataaaccgg ttatacctgc cgctggaaaa actgaagaac cagatgtgcc gaaacccctt
      481 tttatggatt caacaagccc tgtgataaca aaaataaaaa ttatacaact tgctccttca
      541 agaacagtga agttctccct tttccaggat ttagaccagg cgtaaagaaa aacagcaatg
      601 acacataagt atgcaaccca tccgaaaaga aaaaacatta aggcagacat atttgcgcca
      661 aagttgccac caaaatttgc tggcacatct ttcacaggat atcccagctg gcagaactga
      721 aaacagggat catcaggact gtatgtaacc aggcacagaa agagaaatat cgtcactccc
      781 agaagaataa tacccacgaa tcttgagaaa tttctttcca tcttttcaat tttaacataa
      841 aataaataaa tcgttaacag ggagattcaa tgtcatttga aaatcaaaac agaagaaatc
      901 tgaaatttgc cagtactctg aaacatcaga tcaatgaaat tttgcaaaaa gaaatttctg
      961 atccaagaat aggatttgta acaatcacaa atatcagggt aagcgcagat agaagacatg
     1021 tgacagttta cgtaagcaca ctcgggaatg aagaaaaaca gacagaatcc ctgaaaggac
     1081 tcgccaatgc gtcaggatat atcagacatc ttttaagcaa gaaacttgaa accagattta
     1141 ctcctgaggt tgaatttgta aaggatgaaa atccaggcaa tagaattgaa gagatactga
     1201 aagaaattga acaagacaaa ggaaaagaac aatgacctct tctgacataa aagaaaaact
     1261 tataaagatt ttaacagatg caggattaca ctttgaacaa ttcagtgtat ttcctgcagc
     1321 aaaaaaagaa tttggcgact tttccacaaa cttatttttc aagttaaagg aaactgcaaa
     1381 tcgcgtttca atcaaagatt taatttccct ggtgatgcag gacacctgga tgacgaagca
     1441 ctttgaaaaa atcgaagaaa aaaatggttt cttgaatttt ttccttaaag atactctgat
     1501 ttacggagtc atcgatgaga tactcacaaa gaaacaaaca tttggaaaat ctggctgtgg
     1561 aaacaacaca aaagtacttg tagaatttgt cagcgccaat ccaacaggac cacttaccat
     1621 agcacatgga agacaggcag ccttcggaga gtcccttgca agaattcttg ctttttctgg
     1681 atggaaggtg accagagaat actatctgaa tgattgtgga agacagatgg agcttcttgg
     1741 aatttcttta aaatgtcgtt atcttcaaat gaaaggcata tctgctgaaa tccctgagga
     1801 gggatacctt ggagaatacc tgaaggacat cgcagaaaaa ttacctgacg gctgtgagaa
     1861 taaagatgcc agtttttttc aagaaattgc caaacaagaa atactcaata tgattcgaca
     1921 ggaccttgac caattcaatg tcagatttga caactacttt tcagaaaaac aattgcgcga
     1981 tagcggcaaa gtcgaaaaaa ttattcagca acttaaggaa aattctcttg tgtatgaaaa
     2041 cgaaggtgcg ttgtggttca aatcaacaat ttatggggat gacaaagaca gggtgttgat
     2101 aaaaagtgat ggttcgtata catatcttgc gcctgacatc gcctatcatg aagataaaat
     2161 aaaaagggga taccatcttc tgattaattt atggggacca gaccatgcgg gatatattcc
     2221 aagaattaaa gcggctgttg ctgcgcttgg atttttccct gaaaaactcc agattattat
     2281 cgtgcaactc acaacgctgt accgtggtaa agtaaaaatc tcaatgtcca caagaaaagg
     2341 ccagtttatc accctcaggc aattaatgga tgaggttggc ccggatgcaa caaaattctt
     2401 tttcctcttc agaaaggcag atagtcatct tgactttgat cttgaacttg caaagaaaaa
     2461 aacagaagaa aatcctgtgt actatattca gtatgcttat gtccgagcca tgcatatcat
     2521 tgcgtttgga atagaaaagg gattcaaaga aaacgatatt ctgcgtgcgg acttttcatt
     2581 gttaaaagaa agtcaggaga ttgaaatttt gaaaaaactc aaggcatttc ctgttgtcgt
     2641 cgaatatgcc gccagaactc ttgagatttc gagaatcgcc cattatatcc ttgaccttgc
     2701 aggactcttt cattcgtatt atcaaaagtt cagggtagtt gacgaaaaca aaaaattgtc
     2761 ctgtgcccga cttggactgg tgcaatgtgt gcacacagta atgcaaaacg gtctcgacct
     2821 tcttggcata tccaaaccag aaaaaatgta acatagaatt tgtccttgac acagaaacct
     2881 ttttgaggta atgtattttt gtatgccaac aaagaaagaa attacaacaa tcatccagaa
     2941 cataggactc tggaacaaac aggcaattga gaaagccctg caggaacagg aaactaccga
     3001 tagaccttta aaagaaatat ttcaaaatat ggggcttttg ccttttggag atgtacccgc
     3061 aacactgttt ttccagctcg gtattgttca gcaaagtctc cctttgcgcg aaatccctcc
     3121 tgatgtgata agtctcatat ccccttcatt tgcaaaaaag cacagagtca taccatggga
     3181 gattcgtgaa gaccagaaac ttgttatggt aaccgatgac cccattaaca tcatagccag
     3241 tgactatttc aaaaaaaacc tcaatgttcc ttacgaaatt gaaattatcg ttacttttcc
     3301 aaatgaaata gaccagttga tagcaaaata ctttgaacag caggatattg aagacctctc
     3361 aggtatgctt caagaagttg gaagcgcaga cacgattgaa acgcttgaag atgaagaaat
     3421 tacaactgac gaagacgaag aagcccttgc gcttcaggcc cctgtcgtaa agattgtgaa
     3481 tttaattttt gttgaagcgc tgagaagacg ggcgagcgat attcacctgg aaccgcttga
     3541 aaaaaagttc agggtgagat ttagaattga tggtgtcctt tatgaagttg taagcccccc
     3601 aaaaagagtt gaaagagcag tgattgcacg tgtaaaactg atgtcaaaga tggaccttgc
     3661 agaaaaacgg cttccccagg atggcagaat aatgcttgac ataggtggaa ggcccatcga
     3721 ttttcgtgta tcaacactac caggaattta tggagaaagc gttgttctca gaattcttga
     3781 caaaacaaaa gcactgaaag atcttgagca acttggattt atggaacagg accgtaaaac
     3841 atgggaaaat ctcctgaaat attctggtgg aattttactt gttacaggtc ctacagggag
     3901 cggaaaaaca a
//
LOCUS       DAORYU010000133         3906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_15546_length_3906_cov_4.527655, whole genome shotgun sequence.
ACCESSION   DAORYU010000133 DAORYU010000000
VERSION     DAORYU010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15546_length_3906_cov_4.527655"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PLW07_05010"
     CDS             <1..185
                     /locus_tag="PLW07_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49918.1"
                     /translation="FIGIGIKNLSMAPAAIPQIKQLIRRISYERAKELSEKVFIFSTH
                     KEIFEFLKKNIEKDLL"
     gene            182..1228
                     /locus_tag="PLW07_05015"
     CDS             182..1228
                     /locus_tag="PLW07_05015"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF006426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HOL49919.1"
                     /translation="MILDNRSMIKKIDKSGMLNLLEKFAVQCMDADSIKISPVVAQEY
                     DCIVWAGMGGSAIAGDILKQVVERNCSIPMFVHRNYGLPKFVSNKSLVLAISYSGDTE
                     ETLDACTEAVKRKATVWTLSSGGKLEKFAKNNKIPHAKIPAGQPPRCSLGYLFFPAAN
                     LFFMLGYIKKIVVEKIAEIADACVKKYGVDAVDNNLAKEFARKIHNKNAVIYSGDFLL
                     PVAIRWKTQFAENSKHMVSVNVFPEMNHNEIMAWNFPIQYVENSIVLFLYDRNDHQRT
                     KLRMDLTADILKKKSILVEKLESDESEPVQRMFSLLLLGDWVSFYSALLNGVDPTEIK
                     EISLLKKELTFTNQ"
     gene            1245..2381
                     /gene="metK"
                     /locus_tag="PLW07_05020"
     CDS             1245..2381
                     /gene="metK"
                     /locus_tag="PLW07_05020"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOL49920.1"
                     /translation="MKEFYFTSESVTEGHPDKICDQVSDAILDDVLRQDVNGRVACET
                     LATRGLLFIAGEITTDGYVEIPSLVRNILKEIGYVDKEYGFNYDASAIITAIQEQSPD
                     IAQGVDIGGAGDQGMMFGFATRETPQLMPLPIMLAHALVKRLAEVRKNKILGYLRPDG
                     KSQVTVRYFDEKPVEVTAVVLSSQHDEGISQEKIREDLRQTVVEQVIPAELLAKDYHL
                     FVNPTGRFVIGGPQADTGVTGRKIIVDTYGGFGKHGGGCFSGKDPTKVDRTASYFARY
                     LAKNIVASGVAEKCEIQLSYVIGQKQPISIMLNMFNTEKVPAEKIIQIIRKNLDLSPS
                     GMIKKLNLLRPIFRKTACYGHFGREEEEFTWEKTDIADLFAGAM"
     gene            2406..3650
                     /gene="ahcY"
                     /locus_tag="PLW07_05025"
     CDS             2406..3650
                     /gene="ahcY"
                     /locus_tag="PLW07_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOL49921.1"
                     /translation="MCCDIKDMKLADQGRERIEWAVREMPVIRKLRAEESSSLFKNRI
                     IGACLHITTETANLVITLKKCGAEVYLTASNPLSTQDDVAACLVKDYGISVFGIKGEN
                     SRTYLQHIKEVAAKNVHLVIDDGADLIAFLHNNPSLAKNVIGATEETTTGVTRIKNIE
                     REGKLLFPVIGVNEALTKYMFDNRYGTGQSTIDGILRATNTLIAGKTVVVCGYGWCGR
                     GIAKNLKGLGANVIITEVSPLRALEAVMDGFRVLPIEEAACYGDIFITATGNAKIITG
                     NVFNRMKDGAILANAGHFNVEIDIETLQKISRKISEVKPFVKEFILKTGKKIYVLAEG
                     RLVNLGCAEGHPSSVMDMSFAGQFLSLKYFVNNPGLPVKYHTVPGEIDRKIALLKLNS
                     MGVRIDTLTSYQKKYLNSWNLE"
     gene            3650..>3906
                     /gene="coaE"
                     /locus_tag="PLW07_05030"
     CDS             3650..>3906
                     /gene="coaE"
                     /locus_tag="PLW07_05030"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008288369.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOL49922.1"
                     /translation="MISIGLTGVFGSGKSTVSQIFRKMGIPVISCDVIVNRLLKSEKI
                     KEKIAETFGYEYFLKNGEIDKKKLAGLVFSSSIKREHLNKI"
BASE COUNT         1284 a          615 c          912 g         1095 t
ORIGIN      
        1 tttttatagg aattggaata aaaaatctca gtatggcgcc tgctgcgatt cctcagataa
       61 aacagttaat acgcaggatt tcgtacgaaa gggcaaaaga attgtctgaa aaagttttca
      121 ttttttccac gcataaagag atatttgagt ttttgaagaa aaacatagaa aaggacctgc
      181 tatgatactg gacaatagaa gtatgataaa aaaaattgat aaaagtggaa tgctaaatct
      241 tcttgagaaa tttgctgttc aatgcatgga tgccgacagt attaagatat cacctgttgt
      301 cgcgcaggaa tatgattgta ttgtctgggc tggtatggga ggttctgcca tagcagggga
      361 catactgaaa caggttgttg aacgaaattg ttctatacca atgtttgttc acaggaatta
      421 tgggctgccg aagtttgttt caaacaaatc tctggttctt gcaattagtt attccggaga
      481 cacagaagag acccttgatg cctgtaccga agctgttaag cgcaaagcaa cggtctggac
      541 cctttcgagt ggcggaaaac ttgaaaaatt tgcaaagaac aacaaaatac ctcatgcaaa
      601 aataccggct ggacaacctc ccagatgcag tttagggtat ctatttttcc ctgcggcaaa
      661 ccttttcttt atgcttgggt atatcaagaa aattgtggtg gaaaaaattg cagaaattgc
      721 tgatgcatgc gtgaaaaaat atggtgttga tgctgtggat aacaatcttg cgaaagaatt
      781 tgcaagaaaa atacataata aaaatgctgt gatatattcg ggggattttc ttttaccagt
      841 ggcaatcagg tggaagacac aatttgcaga aaatagcaaa catatggttt cagtcaatgt
      901 gtttcctgaa atgaatcata acgagatcat ggcgtggaat tttcctatcc agtatgtaga
      961 aaacagcatt gtgctcttcc tgtacgatag aaatgatcac cagaggacaa aactgagaat
     1021 ggatttgacg gctgatatac tcaaaaaaaa gtctatcctg gttgagaaac ttgaaagcga
     1081 tgaatcagaa ccagtacaga ggatgttttc acttttgctt ctcggggact gggtcagttt
     1141 ttattcagca ttattgaatg gagtagaccc gactgaaatc aaagaaatat cgttgttaaa
     1201 aaaagaactg acattcacaa atcaataata caaggggtgg agaaatgaaa gaattttatt
     1261 tcacatcaga atctgtaaca gaaggacatc ctgacaaaat atgcgaccag gtgtcagatg
     1321 caattctgga cgatgtgctg agacaggatg ttaatggtcg ggttgcatgt gaaacacttg
     1381 ccacgagggg acttttgttt attgcaggag aaattaccac agatgggtat gtggaaatac
     1441 cgtctcttgt tagaaacatt ttgaaagaaa taggctatgt ggataaagaa tatgggttta
     1501 attatgacgc gagcgctatc attacggcaa ttcaggagca atctccagac atcgctcagg
     1561 gtgttgatat cggaggggca ggtgaccagg gaatgatgtt tggatttgcc acaagggaaa
     1621 cccctcagtt gatgccgctt cctattatgc tggcgcatgc tctggtaaaa agacttgcag
     1681 aggtgagaaa aaacaagatt ctgggttatc tcagacctga tgggaaatct caggttacag
     1741 tgagatattt tgatgagaaa cctgtggagg tgacagcagt tgttctttct tcgcagcatg
     1801 acgaaggtat ttcgcaggaa aaaatcagag aggatttgag acaaacagtt gttgaacagg
     1861 tgatacctgc agaattactt gcaaaagatt atcatttgtt tgtaaatccc actggcagat
     1921 ttgttatcgg tgggcctcaa gctgacacag gagttactgg aaggaaaatt atcgtggaca
     1981 cctatggcgg ttttgggaag cacggcggtg gatgtttttc aggaaaagac ccgacaaagg
     2041 ttgatagaac tgcttcttat tttgcaagat atcttgcaaa aaacatagtg gcttcagggg
     2101 tggcagaaaa atgtgaaatt caactttcgt atgtaatcgg tcagaagcag ccgatttcca
     2161 ttatgctgaa tatgtttaat actgagaagg taccggcaga aaaaatcatt caaattatac
     2221 ggaaaaatct tgatttatct ccgtctggca tgataaaaaa actgaatctt ctcaggccaa
     2281 tttttagaaa aacggcctgt tatggacact ttggtagaga ggaagaagaa tttacctggg
     2341 agaaaacaga tattgccgat ctttttgcag gcgcaatgta atagggggtt catttgaaaa
     2401 aaagaatatg ttgtgatatt aaggatatga aacttgccga tcagggcagg gaaagaattg
     2461 agtgggcagt tcgtgagatg ccggtaataa gaaaactgcg tgcagaagaa tcatcatcgt
     2521 tattcaagaa tcgtatcatc ggtgcatgcc ttcatattac gactgaaact gcaaatcttg
     2581 ttatcacatt gaagaaatgt ggagcagagg tttatcttac cgcatcaaac cctttaagca
     2641 cccaggatga tgtagcagca tgtcttgtaa aggattacgg aatttctgtt tttggaatca
     2701 agggagaaaa cagcaggacg tatttgcagc atatcaaaga agttgcagcg aaaaatgttc
     2761 accttgtcat tgatgatgga gcggatttaa tagcgtttct acacaataat ccatctctgg
     2821 cgaaaaatgt aattggcgcg acagaagaaa ctacaacagg cgttaccaga attaagaaca
     2881 ttgaaagaga aggaaaactt cttttccctg tcattggagt aaatgaagca ctcacaaaat
     2941 atatgtttga taatcgatac gggacaggtc agtcaaccat agatggtatt ttgagagcaa
     3001 ccaatacatt gattgcagga aaaaccgttg ttgtgtgcgg atatggttgg tgcggcaggg
     3061 ggattgcgaa aaacctcaaa ggacttggtg cgaatgtcat tataacagaa gtttctcctt
     3121 tgagagcact tgaagcagtg atggatggtt tccgggtttt accaatagaa gaagctgctt
     3181 gttatggcga tatatttatc accgctacag gaaatgcgaa aattattact ggcaatgttt
     3241 tcaatagaat gaaggatggt gctattcttg ctaacgcagg acattttaat gttgagattg
     3301 atattgaaac actgcagaaa atatcaagaa aaatttctga ggtgaaacct tttgtgaagg
     3361 agttcatttt gaaaacaggg aagaaaattt atgttcttgc tgaggggcgc cttgtgaatt
     3421 taggttgcgc cgaaggacac ccttcatctg ttatggacat gagttttgca ggacaatttc
     3481 tgagtttaaa gtattttgtg aacaatccag gattaccagt aaaatatcat acagtacccg
     3541 gtgaaattga cagaaaaatt gcactgttga aattaaacag tatgggtgta aggatagaca
     3601 ccctgacatc gtatcagaaa aaatatctca attcctggaa tctggagtaa tgattagtat
     3661 tggtttgaca ggagtttttg gatcaggaaa atcaacggtt tcccaaattt tcaggaaaat
     3721 gggtatcccg gttatttcat gtgatgttat tgtgaatcgt cttttaaaat cggaaaaaat
     3781 aaaagaaaaa attgctgaga ctttcggtta cgaatatttt ctcaaaaatg gagaaattga
     3841 taaaaaaaaa ctggcgggac ttgtattttc ttcgtcaatc aaacgtgagc atcttaacaa
     3901 aattat
//
LOCUS       DAORYU010000134         3866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_15741_length_3866_cov_4.822356, whole genome shotgun sequence.
ACCESSION   DAORYU010000134 DAORYU010000000
VERSION     DAORYU010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15741_length_3866_cov_4.822356"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1683)
                     /locus_tag="PLW07_05035"
     CDS             complement(<1..1683)
                     /locus_tag="PLW07_05035"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOL49923.1"
                     /translation="MKINENLDSSVRLEYDKLLNPEQTQAVREAEGPCLVLAGAGSGK
                     TRVLTYRAIYLIEQGIPPSRICLLTFTNKAAREMVRRIENHFGLLPEGILAGTFHHVA
                     NVFLRRYTRYASLPSNYVIIDREDALSIVREVSKTVFTEKEISPELAQEFFSLSANTS
                     ETIKDLVMTQYPHFSHIISRLESLHDAYIRRKRELGVVDYDDLLSFWLKILSMQEPGE
                     KISRKLLYILVDEYQDTNRLQALILYQLARINKNIMVVGDDAQSIYSFRGATIQNILE
                     FPKIYPDTKFFYLQTNYRSTPEILNLANSVISHNRNQFPKILKSVKNSGVKPVVVECQ
                     DKHSECLFVIRRIQQLLKNVKPSDIGILFRSRYQSAEIEIELNKLNIPYVVRGGLRFF
                     EMAHIKDVIAFFRVYHNPKDELSWVRILNLIRGIGKSSRSKIVDMMKEKKTIEEVISS
                     DGIKLIAPAQSGWKIVKNIFKNMKDTEQISQQITVIMDSFYRAYLEPNYLDSQQRIAD
                     VEGLKEIASTWNNVEEFISQTSLQEHYRGETTQTSPLILSTIHQAKGLEWKIV"
     gene            complement(1680..2603)
                     /locus_tag="PLW07_05040"
     CDS             complement(1680..2603)
                     /locus_tag="PLW07_05040"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOL49924.1"
                     /translation="MNWKRKDLLGLEELSRQEIEFILDTAGSFKEVSTRAVKKVPALR
                     GKTIVNLFFEPSTRTKTSFELAAKRLSADILSFEVSTSSVSKGETIVDTARNIEAMKV
                     DCFIVRHSVSGAPLIISKAVKAAVINAGDGCHEHPTQALLDLFTVKEVFGKISGLNIA
                     IIGDIYHSRVARSNIWGFTKLGAKVTVCGPPTLIPHGVEQTGVSICYSVAEAVKNADI
                     VYLLRIQKERQQENFFPSQAEYRNFFGFDYKKFQFQKNKFFLMHPGPMNRGLEISTES
                     AEKENSLILQQVTNGIAVRMAVLYLTVRQVH"
     gene            complement(2600..3130)
                     /gene="pyrR"
                     /locus_tag="PLW07_05045"
     CDS             complement(2600..3130)
                     /gene="pyrR"
                     /locus_tag="PLW07_05045"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOL49925.1"
                     /translation="MENKILSTNEIKKILEKLSDQVIKEMGPDIAVVGIKRRGAILAE
                     RIKKLVERKVSCNIDYGSLDITLYRDDFSTIGSHPVVSGSELMFDPDDRKILLVDDVL
                     YTGRTIRAAIDQIIDYGRPNVIRLFVLVDRTNCRELPIQADYHGIKVIVGKNQIVEVK
                     LKEVDKTEEVVIAEIK"
     gene            3235..>3866
                     /locus_tag="PLW07_05050"
     CDS             3235..>3866
                     /locus_tag="PLW07_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49926.1"
                     /translation="MTKREKLLRCLELNGDGEIFSSVSIAWTVWEKHLEFFQEKVKKC
                     PHVSISGGKDETRRAGKKVRDKWGCLWIYPMDYLDGQVIEHPLDDWSKLQYYVAPDPE
                     EYTDWEKAKKDVQDAKSKGILASGGIDHGFFFLLLTYLRGYENAMIDFAEDNKNLHQL
                     IEIIENYWMKVIEKWINIGVDFIGFGDDLGLQHSLPISPNAWRKYIKPSY"
BASE COUNT         1091 a          763 c          722 g         1290 t
ORIGIN      
        1 aacaattttc cactcaagtc cttttgcctg atggattgtt gacagtatca ggggagatgt
       61 ttgagtggtt tctcccctgt aatgttcctg caaacttgtc tggcttataa actcttcaac
      121 attattccat gtgctggcaa tttctttcag accttcgaca tctgcgatgc gctgttgtga
      181 atcaagataa ttgggttcaa ggtaagcacg ataaaaactg tccataataa cggtaatttg
      241 ttgagagatt tgttctgtgt ctttcatatt cttaaaaatg ttcttgacaa ttttccatcc
      301 tgattgagca ggcgcgatca attttattcc gtcagaggaa ataacttcct caatcgtttt
      361 cttttccttc atcatatcaa cgatcttgct tcttgatgat tttccaatac ccctgatgag
      421 attaaggatt ctgacccagg acagctcgtc tttcggattg tgataaactc tgaaaaaagc
      481 aatcacatct tttatgtgag ccatctcaaa aaatcttaat ccgcccctga ccacatatgg
      541 aatgttcaat ttgtttaatt caatttcaat ttcagcggat tgatatcttg atctgaaaag
      601 aataccgata tctgatggct ttacattttt caggagttgt tgaattcttc ttataacaaa
      661 caaacattca ctgtgtttat cctgacattc cacaacaaca ggttttactc ctgaattttt
      721 cacacttttc agtattttcg gaaactgatt tctgttatgg gagatgacac tgtttgcgag
      781 atttaatatc tcaggggtgc ttctataatt ggtctgaagg tagaaaaatt ttgtgtcagg
      841 atatatcttt ggaaattcaa ggatgttttg aatggtggct cctcggaaac tatatatact
      901 ttgggcatcg tcacccacaa ccatgatatt tttgtttatg cgtgccagtt ggtaaagtat
      961 taatgcttgc agtctgtttg tatcctgata ttcatcaaca agtatgtaaa gtaattttct
     1021 ggatattttt tctcctggct cctgcattga aaggattttc aaccagaatg aaagaaggtc
     1081 atcatagtca actaccccaa gttctctttt tcttcttatg tatgcgtcgt gaagtgattc
     1141 taatctggaa ataatatgag aaaaatgggg gtattgtgtc attaccaggt cttttattgt
     1201 ttcactcgta tttgcagaca agctgaaaaa ttcctgagca agttctggtg aaatttcctt
     1261 ttccgtaaat actgttttcg atacttctct tacaattgaa agggcgtctt ctctgtcaat
     1321 gataacgtaa ttggacggta atgaagcata tctggtgtat cttcgcagaa atacatttgc
     1381 gacatgatga aatgtgcctg cgagaattcc ttcgggcaat aaaccaaaat gattttcaat
     1441 ccttcttacc atttcccttg ccgccttatt ggtaaatgtc aaaaggcata ttcttgatgg
     1501 tggaattccc tgttcaataa ggtatatcgc tctataggta agaacccttg ttttacctga
     1561 acctgctcct gcaagaacaa ggcacggacc ttctgcttct cttactgcct gtgtctgttc
     1621 aggattcaac aacttatcgt actcaagacg tacgctggaa tcgagatttt cattgatttt
     1681 caatgtacct gcctcactgt aaggtataga acagccattc tcactgcaat accattggta
     1741 acctgctgca aaatgagaga gttttctttt tctgcacttt ctgttgatat ttccagtccc
     1801 ctgttcatcg gtccagggtg catcaagaag aatttgtttt tttgaaactg gaactttttg
     1861 taatcaaaac caaaaaaatt tctgtattct gcttgagaag ggaaaaagtt ttcctgctgt
     1921 ctttcctttt gaattctcaa aagatataca atatccgcat ttttcactgc ttctgccact
     1981 gaataacaga tggagacacc tgtttgttca accccatggg gaatcaatgt gggcgggcca
     2041 caaacagtga cttttgcgcc taattttgta aatccccata tatttgatct tgccaccctg
     2101 ctatgataga tatcgccaat aatggcaatg ttcaatcctg agattttgcc aaagacctct
     2161 ttcactgtaa acaggtcaag aagtgcctgc gttgggtgtt catgacaacc atcgccagcg
     2221 ttaataacag ccgccttaac ggctttactg ataatcaatg gtgctccgga tacagaatgc
     2281 ctgacaataa aacaatcaac cttcattgct tcgatatttc ttgctgtatc cactattgtt
     2341 tctcctttgg atacacttga tgtactgact tcaaaactta aaatatctgc tgacaatctt
     2401 ttcgccgcca gttcaaacga tgtttttgtt ctcgtgctcg gttcaaaaaa aagattcacg
     2461 atggttttgc ctctaagagc agggactttt ttcactgcgc gcgtgctgac ttccttaaaa
     2521 gagccagcag tgtcaagaat aaactctatt tcctgtcgtg aaagttcttc aagtccaagc
     2581 aaatcttttc ttttccagtt cattttattt ctgcgatcac aacctcttca gttttatcaa
     2641 cttctttcag tttgacctca acaatttgat tttttccaac aatgacttta atgccatgat
     2701 agtctgcctg tatcggtagt tctctgcaat ttgttctatc tacaagaaca aaaagtcgaa
     2761 tgacgtttgg tcttccataa tctataatct gatcaattgc tgctctgatt gttcttcctg
     2821 tgtacagaac atcatctacc agaagtatct tgcgatcgtc agggtcaaac atcagttcag
     2881 aaccagatac cacaggatgg cttcctatgg tactgaaatc atccctgtaa agggtaatat
     2941 caagagatcc ataatcaatg ttacaggaaa cctttcgttc aaccagtttc ttgattcgtt
     3001 cagcaagtat ggcaccgcgg cgttttattc cgacaaccgc gatgtcgggt cccatttcct
     3061 tgattacctg gtctgacagt ttttcaagga tttttttgat ttcatttgtt gaaaggattt
     3121 tattttccat atacttattt taccccaaaa cccgcaatta aaaaaccagt agcagtttaa
     3181 aatatcaatt tgtgataaaa ttcatataac tgcttcacaa attgaggagg aattatgaca
     3241 aaacgggaaa aattactcag gtgtttagaa cttaacggag atggtgagat tttttccagt
     3301 gtttcaattg catggactgt gtgggaaaaa catctggaat tttttcagga aaaggtgaaa
     3361 aaatgtccgc atgtctcaat ttcagggggt aaagatgaaa cccgtcgtgc aggcaaaaaa
     3421 gtcagggata aatggggttg tctatggatt tatcctatgg attatcttga tggacaggtc
     3481 attgaacatc ctctggacga ttggtctaaa ctccaatatt atgttgcgcc tgaccctgag
     3541 gaatacactg actgggaaaa ggcaaaaaaa gatgttcagg acgcaaaaag caaaggtatt
     3601 ctcgcatcag gaggaattga ccatggtttc ttttttctcc ttcttacata cctgagggga
     3661 tacgaaaatg caatgattga ttttgctgaa gataataaaa atcttcacca gttgattgaa
     3721 ataattgaaa attactggat gaaggtgatt gagaaatgga taaatattgg tgtagatttc
     3781 attggattcg gagatgacct tgggcttcaa cattcacttc ctatcagtcc gaatgcctgg
     3841 agaaaatata tcaaaccatc gtataa
//
LOCUS       DAORYU010000135         3866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_15746_length_3866_cov_3.358699, whole genome shotgun sequence.
ACCESSION   DAORYU010000135 DAORYU010000000
VERSION     DAORYU010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15746_length_3866_cov_3.358699"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..1482)
                     /locus_tag="PLW07_05055"
     CDS             complement(112..1482)
                     /locus_tag="PLW07_05055"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOL49927.1"
                     /translation="MDKYRISELQDEFKNILRVLETTESIDKNFFIPEEEFLGRQKKI
                     YEETKKHGIDAGIVFSDEHYNGDVPYLGGNTNVTVEQVAGVIGKTGFHIIAGLEGGYV
                     SEQLAPRACAKVHKVEMLKLADEEYPINAEKLECVIEQAAGCKARKIGLLTPREVIPY
                     RLVKYLEDIYGTENVVDIQEVYYKIKYEKSDNEMKLIRDACRIADAMMRGMLAILKPG
                     LLETQVAAWGYFIARELGAEELGWDIIVGANEANRTLIGKALNREIKKGDYVHLGVAP
                     KRDGLNSCIRRSVIAVENPSEVTEEQKYWFNLVEEAYRIGFEKYCEVAEKNLPAYLQE
                     KALVDYFASRSEEVSRRIGKKINLEKLKPYTGTHNAGYTECQEFYGAITLSSSQPLGH
                     QIVTMLDVALRGIGDKWDDVIIPGFDYVVVENTLGKFGKWVECLNKLPINVQSLVGNP
                     EPPNQD"
     gene            complement(2049..>3866)
                     /gene="asnB"
                     /locus_tag="PLW07_05060"
     CDS             complement(2049..>3866)
                     /gene="asnB"
                     /locus_tag="PLW07_05060"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOL49928.1"
                     /translation="HRGPDEEGLQLFDNVGLGHRRLRIIDVNTGKQPMSNENNTIFLI
                     CNGEIYNFRDLRNLLEARGHIFKTKSDNEVILHLYEQHGTGCLKFLRGMFAFAIWDDR
                     NKTLFLARDRLGKKPLVYSIKDQSIYFASEIKALLEVEEISREIDLKAIDLFLTYQAI
                     PAPWTIFKEIKKIPPAHYLVWKEGQVKIERYWQIDFTRKLTLKDEQEYIDAMWEKLVE
                     STRLRMIADVSLGAFLSGGIDSSTIVGIMSENSKEPVKTFSIGFEETDFSELHYARIV
                     AQKFATNHNEFIVKPDVISILPELVWYYNEPFADSSMVPTYYVARETRKHVIVALNGD
                     GGDENFAGYTRYWQTKLLEKIYHAYKRTPFMRKETLRILTKLHEKIPSNTFFRIWKWL
                     EETEKYGFDYAYARRLISFTRDFRLGIYSEEMKKEIADFDSLVLVEKIWDNSGDIDIL
                     EKMLATDFQVYLPDVLMVKMDIATMANSLEGRSPFLDHEFVELVASFPPQLKMKNLKS
                     KYLLKKRLRNFLPDEIVSRKKMGFGIPVGKWFRCELKNFLQDILLDKHALSRGYFKSE
                     LVRNMVEQHVARSMDHTSRLWSLVMLEMWHKIFIDKKSF"
BASE COUNT         1138 a          826 c          569 g         1333 t
ORIGIN      
        1 ctgttagtgt cggcgtggcg tactaccagc cggggcataa acaaaccatt gacaacctta
       61 tttcagttgc agataagttg atgtatgcga ataaacgtct taagaaaaaa actaatcctg
      121 attaggcggc tcagggtttc caacaagcga ttgaacatta ataggaagtt tatttaaaca
      181 ttccacccat ttaccgaact tgcccagggt attttcaact acaacatagt cgaaacctgg
      241 aataatcaca tcatcccact tatcccctat tccacgaagt gcaacatcaa gcatagtaac
      301 aatctgatgc ccgagtggct gagaagatga aagtgtgatt gctccataaa attcctggca
      361 ttcggtataa ccagcgttgt gtgtgccagt ataaggttta agtttttcaa ggttaatctt
      421 ttttccaatt cttctggata cctcttctga tctggaagca aagtaatcaa caagtgcttt
      481 ttcctgtaag tatgccggca gatttttttc agcaacctca caatattttt caaatccaat
      541 tctgtatgcc tcctcaacaa gattgaacca gtatttctgt tcttcggtca cttcagacgg
      601 attctctact gctattacag accttcttat acaggaattg agcccatctc tttttggcgc
      661 aacaccaaga tggacataat ctcctttttt tatctctctg ttgagcgcct ttccaataag
      721 ggtacggttt gcttcattgg ctccaacaat aatatcccat ccaagctctt cagccccaag
      781 ttcccttgct ataaaatatc cccacgcagc aacctgtgtt tcaagtaaac ctggttttaa
      841 tattgcaagc atacccctca tcatggcgtc agcaatacga caggcatccc ttatcagttt
      901 catctcatta tcagattttt catacttgat tttgtagtag acctcctgaa tgtcaacaac
      961 attctcagtg ccataaatat cttcaagata tttaacgagt ctgtatggta tgacttctct
     1021 tggagttaaa aggccaattt ttcttgcctt acatcctgca gcctgttcaa ttacacattc
     1081 aagtttttca gcattgatag gatattcttc atctgccagt ttcagcatct ccactttatg
     1141 aacttttgca catgctcttg gcgccagttg ttctgacaca tatccacctt caagacctgc
     1201 aataatatga aagcctgttt tgccaataac tccagcaacc tgttcaacgg taacattcgt
     1261 attacctcca agatatggca catccccatt ataatgctca tctgaaaaaa cgattcctgc
     1321 atcaatcccg tgtttcttgg tttcctcgta tatctttttc tgtcttccaa gaaattcctc
     1381 ttcaggaata aaaaaatttt tatcaattga ttcagtggtc tcaagtaccc ttagtatgtt
     1441 tttgaattcg tcctgcaact ccgatatcct gtatttgtcc atcgtactct ccttttcatt
     1501 taagtatctt atagcatttg attgactttt aataataatt ttattcttta accaataaat
     1561 aagcaaaaca aaagttcaaa aacagaagcg cccgggaaaa actcaaaaaa ccgggcgctc
     1621 aattcaaact actttttctt tttcttcgct gtctttttag ctgttttctt tgcggtcttt
     1681 tttaccgttt ttttcttggc tgtttttttt gcacacgcca ttcaaatcac ctccttcagg
     1741 gtaaaataaa aacaaataaa tttaccatta aaacaatcat cggacttctt aagaatacag
     1801 gtcaatcctt ttgcactcaa tggaaacaca aaatcagtga ttctaatttt tattttattt
     1861 ttgatcaatc tctgtcctgt aaaaacaaaa atcaggtaac agattttttt gatttaagga
     1921 accgttaagg taggcatttt cagatgaggg acaccgctga agatttttac ctattccaaa
     1981 tttttttatc tttttatttt cctccattta taaaaaatat aacacaaatt ttttttaatg
     2041 caaatttttt agaaggattt tttgtcaata aaaattttat gccacatttc aagcattacg
     2101 agagaccata gtctgctggt gtgatccatg cttcttgcaa catgttgttc aaccatattt
     2161 ctgaccaatt ctgacttgaa atatcctctc gatagggcat gtttgtctaa aagaatatcc
     2221 tgaagaaaat tcttcaattc gcatctaaac catttaccaa ccgggattcc aaaacccatt
     2281 ttttttcttg aaacaatttc atcgggtaga aaatttctca atcttttttt cagtaaatac
     2341 tttgatttta gatttttcat tttaagttga ggaggaaaag acgctaccag ttccacaaat
     2401 tcatgatcaa gaaaagggct tcttccttca agtgaatttg ccatagttgc aatgtccatt
     2461 ttcaccatca atacatcagg aagataaact tgaaaatcag tggcaagcat tttctcaaga
     2521 atatcaatat ctccagaatt atcccatatt ttttctacaa gaaccagtga atcaaaatca
     2581 gcaatttctt ttttcatttc ttcagaataa atgcccaacc tgaaatctcg cgtaaaagaa
     2641 atcaatcgtc ttgcataggc gtaatcaaaa ccatattttt cagtttcctc cagccatttc
     2701 catattctga aaaaagtgtt tgaaggaatt ttttcatgta atttcgtaag aattcttagt
     2761 gtctcttttc tcataaatgg cgtgcgttta taagcatgat aaatcttttc cagaagtttt
     2821 gtctgccagt atcttgtata accagcgaaa ttttcatccc caccatctcc atttaaagca
     2881 acgatgacat gtttccttgt ttcccttgcc acgtaatatg ttggcaccat tgaagaatct
     2941 gcaaatggtt cattatagta ccagaccagt tcaggtagaa tagaaatcac atcaggtttc
     3001 acaataaact cattgtgatt ggttgcaaat ttttgagcaa caattcttgc ataatgcaat
     3061 tcactgaaat cggtctcttc aaatccaatt gaaaatgttt tcactggttc ttttgaattt
     3121 tcactcatga tgccaacaat cgttgaactg tcaatgcctc cacttaaaaa agctcccaat
     3181 gaaacatcgg caatcattct taatcttgtt gactccacaa gtttttccca cattgcgtca
     3241 atatattcct gttcatcctt caatgtgagt tttcttgtaa aatcaatctg ccagtatctc
     3301 tcaattttca cctgaccttc tttccagacc agataatgag caggcggtat tttttttatc
     3361 tctttgaata ttgtccaggg tgctggaatt gcctgatatg tgagaaaaag gtcaattgct
     3421 ttaagatcaa tttctcttga gatttcttct acttcaagaa gtgccttgat ttcagaagca
     3481 aaataaatac tttgatcttt tatagaataa acaagtggct ttttgcccaa tctatcacgc
     3541 gcaagaaata atgttttatt tctatcatcc catatggcaa aagcaaacat cccccttaaa
     3601 aatttcaagc atcctgttcc atgctgctca tacaaatgca aaatgacttc attatctgat
     3661 tttgtcttga atatatgccc tctggcttca agaaggtttc ttaaatccct gaaattataa
     3721 atttcaccgt tgcatataag aaaaatggta ttgttctcat ttgacatcgg ttgtttgcct
     3781 gtgttgacgt ctataattct taatcttctg tgacccaatc cgacattatc aaacaattgt
     3841 aaaccttctt catctggtcc gcgatg
//
LOCUS       DAORYU010000136         3861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_15763_length_3861_cov_4.517341, whole genome shotgun sequence.
ACCESSION   DAORYU010000136 DAORYU010000000
VERSION     DAORYU010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15763_length_3861_cov_4.517341"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..957)
                     /locus_tag="PLW07_05065"
     CDS             complement(1..957)
                     /locus_tag="PLW07_05065"
                     /inference="COORDINATES: protein motif:HMM:NF036812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOL49929.1"
                     /translation="MKTSNPLRIIDANFNRVREALRVVEDILRFSNAGKNTISDIRKI
                     KHDLCTQYFKTFGYRALATRDITADPGRKHAPYRADTLTQILTRNFMRAEEGLRCIEE
                     CSRIVNPESTDTWQAIRFKIYAIEQQIVTNTPEQTIPHNFSGVYTNTEVALKIMTDIS
                     PDILILNVEENMKTDLKLLKKIKRSSGKTMIFVANRPDIALIANIDGIHLDPDSFAPE
                     DARKIVPGKIIGLAMHDMVLQKFYDAGISYIAFYSCVDISRLLTKHKKNIKLLLAAIV
                     NSRQEAKKAIEKGADGVIVKNDIPQEIIETTGLIKELYGKET"
     gene            complement(944..1423)
                     /gene="rfaE2"
                     /locus_tag="PLW07_05070"
     CDS             complement(944..1423)
                     /gene="rfaE2"
                     /locus_tag="PLW07_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HOL49930.1"
                     /translation="MKSKKIKSLKELQKIVKNLKIRKKKIVFTNGCFDILHAGHLYVL
                     EKAKKTGDVLIVGLNSDESVRKLKGNNRPIMSEKDRAYLLSGLSCVDYCVIFNEETPA
                     QIIQAIKPDVLVKGADYKHGEIVGEDVVKANRGKVLRIPLLKGKSTTNIITKIHENQ"
     gene            complement(1404..2003)
                     /locus_tag="PLW07_05075"
     CDS             complement(1404..2003)
                     /locus_tag="PLW07_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOL49931.1"
                     /translation="MKSTKKLVENIVLEHTELIGFLKTQKFVVQIDKVVCLLVSTVRN
                     GNKILLCGNGGSAADCQHFAGELVGRFLMERKPIACIALTTNSSIITAIGNDYSFDRV
                     FSRQIEATGNKEDTLILISTSGKSASIIDAAKTAKKTGIRTIGLTGSAPNPLSNIVDI
                     CVAVPSTSTPRIQEIHSIIIHILCHAIEQELFSHEVKKN"
     gene            complement(1978..2544)
                     /gene="amaP"
                     /locus_tag="PLW07_05080"
     CDS             complement(1978..2544)
                     /gene="amaP"
                     /locus_tag="PLW07_05080"
                     /inference="COORDINATES: protein motif:HMM:NF033218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HOL49932.1"
                     /translation="MRIFTRIVQTLFILAGLVIVFFLALYWIKPEILIRAQSYLEKNL
                     IKAGLAGFSMILIGLIWLVYWFDHVIRTRSISLDNPHGKVRISLKAIEEFIAKKISTQ
                     MTNITSMNVKASLGSHGLETLMQLRISGGYNIPELTSQIQELIKNYLEDVVGVERVSH
                     IEIVVSSITPPDEEIQKGPTDEIDKETG"
     gene            complement(2541..3659)
                     /gene="dprA"
                     /locus_tag="PLW07_05085"
     CDS             complement(2541..3659)
                     /gene="dprA"
                     /locus_tag="PLW07_05085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOL49933.1"
                     /translation="MMDRKTAWIILNMANGIGPASIRKMLELCKTPQQILDTEPSVFE
                     KLIPAAAIEQLKKWQQLPWEQEIEKAKAAGITVISIDESQYPENLKQIPDPPPVLYVK
                     GKIPQECIFIAIVGTRNPSFYGSTMAEKFASALASYGICIVSGMARGIDSIAHKAALK
                     MKTPTVAVLGSGLANIYPPENEKLAEQISQNGAVISEFPLDTSPEKFNFPRRNRIISG
                     LSKAVIVIEAGLRSGALITAHLAADQNRDVFVLPSDANRITGRGNNQLIKEGACLVES
                     PEEILQQMNLQVRDEIQNSDSSVEHAELSEKEKLVYNVLRRKNLRFEQIQMQVNSPVA
                     EIMQILTNLEIKGFIKSGPGLTYSDNRTVDIKKNEEKI"
     gene            complement(3656..>3861)
                     /locus_tag="PLW07_05090"
     CDS             complement(3656..>3861)
                     /locus_tag="PLW07_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560002.1"
                     /note="dGTPase family type 2 subfamily; presumably
                     hydrolyzes dGTP to deoxyguanosine and triphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HOL49934.1"
                     /translation="RMAEKAARIIKDLFDAYYEEPRQMPPHAYKKIDKESKEQVICDY
                     LAGMTDRFAQEEHKKLFDPHSIV"
BASE COUNT          991 a          777 c          700 g         1393 t
ORIGIN      
        1 tcaggtttct tttccatata gttcctttat caatccagtt gtttcaataa tttcttgcgg
       61 gatgtcgttt tttacaatca cgccgtcagc acctttttct attgcttttt ttgcttcctg
      121 gcgggaattg acaatcgctg caagcaataa tttaatgttt tttttgtgtt tcgtcaagag
      181 ccgggaaata tcaacacaac tgtaaaaagc aatataagaa ataccggcat catagaattt
      241 ttgtaatacc atatcatgca ttgccaatcc tattatcttg ccaggaacaa ttttccttgc
      301 atcctctggc gcgaatgaat caggatcaag atgaattcca tcaatgtttg caatcagtgc
      361 aatgtcaggt ctatttgcaa caaagatcat tgttttccct gaactccttt ttattttttt
      421 caacagtttc agatctgttt tcatattttc ttcgacgttc aatatcagaa tgtctggtga
      481 gatgtctgtc ataattttaa gtgccacttc agtatttgtg taaacacctg aaaaattatg
      541 gggaattgtt tgttccggag tgttagtaac aatttgctgt tctatcgcat aaattttgaa
      601 tctaattgcc tgccaggtgt ctgtactttc gggattgaca attcttgagc attcttcaat
      661 gcatcttaag ccttcttctg ccctcataaa attacgagtc aaaatctgtg tcaatgtatc
      721 tgccctgtaa ggagcatgtt tcctgcccgg atcagcagtg atatctcttg ttgcgagtgc
      781 tcgataacca aaggttttga aatattgagt gcaaaggtca tgttttatct ttctgatgtc
      841 tgaaattgta ttttttcctg cattagaaaa tcttaaaata tcctctacaa cccttaatgc
      901 ctctcttact ctattaaaat ttgcatctat gattctgagt gggttactgg ttttcatgaa
      961 tctttgttat tatgtttgtg gttgatttgc ctttcagaag gggaattctc agtactttgc
     1021 cccggtttgc tttgacgaca tcttctccca caatttcacc atgtttataa tctgcgccct
     1081 ttaccagaac gtctggcttg attgcctgga taatttgcgc aggggtttct tcattaaaaa
     1141 tcacgcaata gtccacacat gataaacctg aaagaaggta tgccctgtct ttttccgaca
     1201 taatgggccg attattgcct tttaattttc tgacagattc atcgctgttt agacctacta
     1261 tcaggacatc tcctgttttt tttgcttttt caaggacata taagtgccct gcatgaagaa
     1321 tatcaaaaca accatttgta aacacaattt tcttttttct aatttttaga ttttttacaa
     1381 ttttttgtaa ttctttcaac gacttaattt tttttgactt catggctgaa taattcttgc
     1441 tcaatggcat ggcagagtat atgaatgatg attgagtgaa tttcctgaat tcttggagta
     1501 gatgttgaag gaacagcgac gcagatgtct actatgtttg ataatggatt tggtgcagaa
     1561 ccagtgagtc cgattgttct tattcctgtt ttctttgctg tttttgcggc gtcaataata
     1621 ctggctgatt ttcctgatgt tgaaattaaa atcagggtat cttctttgtt gcctgttgct
     1681 tcaatctgtc ttgaaaaaac cctgtcaaac gaatagtcat ttcctatcgc tgtgattatg
     1741 gatgaatttg ttgtgagggc aatgcacgct atcggttttc tttccatcaa aaatcttcca
     1801 acaagttctc ctgcaaagtg ctggcagtca gcagcgcttc cgccatttcc gcacaagaga
     1861 attttgtttc cgttcctgac tgtacttacc agcaaacaga caaccttatc aatctgaaca
     1921 acaaacttct gtgtttttaa aaaaccaata agttctgtat gttccagcac aatattttca
     1981 accagtttct ttgtcgattt catctgtggg acctttctga atttcttcgt ctggcggtgt
     2041 tatgcttgac acaacgattt caatatggga aaccctttca acaccgacga catcttcgag
     2101 ataatttttt attagttcct gaatttgaga cgtgagttcc gggatattat aaccgccaga
     2161 aatcctcagt tgcatcaatg tttcaagtcc gtgagaacca agagaggcct ttacgttcat
     2221 tgatgtaata ttggtcattt gagtgctgat tttttttgcg atgaactcct caattgcttt
     2281 caaggaaatt cttacttttc cgtgcgggtt gtcaagagaa atacttctgg tacgaatgac
     2341 atgatcaaac cagtatacaa gccagatgag cccgatcagt atcatagaaa atcctgccag
     2401 acctgctttg atcaaattct tctccagata gctttgtgcc ctgatcaaaa tttcaggttt
     2461 tatccagtac agtgcgagga agaatactat aacaagccct gctaaaataa aaagcgtctg
     2521 aacgatcctg gtaaatattc tcatattttc tcctcatttt ttttaatatc gactgtcctg
     2581 ttatccgaat aggtcagtcc tggacctgac ttgataaacc cttttatttc tagattcgta
     2641 agtatttgca taatctctgc gaccgggcta ttcacctgca tctgaatttg ttcaaaacgt
     2701 aaattttttc ttcttaatac attataaacc aatttttctt tttctgataa ctccgcgtgt
     2761 tcgacggaac tgtctgaatt ttgaatttca tcgcgcacct gtaaattcat ttgctgcaga
     2821 atttcttcag ggctttcaac aagacacgct ccttctttta tgagttgatt gttacccctg
     2881 cctgtaatgc gatttgcatc agatggtaaa acaaaaacat ctctgttctg gtctgctgca
     2941 agatgcgctg tgatgagggc accgcttctt aatcccgctt ctatgacaat gacagctttg
     3001 ctcaatcctg aaataattct atttcttctt ggaaaattga atttttcagg agatgtgtca
     3061 agaggaaatt ctgatattac tgccccgttt tgagatattt gttcggcaag tttttcattt
     3121 tcaggaggat atatgttggc aagaccgctg ccaagtacag ccactgttgg tgttttcatt
     3181 ttcaatgctg ctttatgtgc aattgaatca atcccgcgtg ccatgccact gacgatacag
     3241 ataccgtatg acgccagtgc agaagcaaat ttttctgcca ttgttgagcc ataaaatgat
     3301 ggatttcttg ttcccacgat ggcaataaaa atacattcct gagggatttt cccctttaca
     3361 taaagaaccg gtggtggatc tggtatctgt tttaaatttt caggatattg tgattcatca
     3421 atacttataa cggttatacc agcggctttt gctttttcaa tttcctgttc ccatggtagt
     3481 tgttgccatt tcttcaattg ttcaatagcc gcagcaggaa taagtttttc aaatactgag
     3541 ggttctgtat ccagaatttg ctgtggggtt ttgcacagtt ccagcatttt tctgatactt
     3601 gcaggcccga ttccattagc catgttaagt attatccagg cagtttttct atccatcata
     3661 cgatactgtg tggatcaaaa agttttttat gttcttcctg cgcaaacctg tctgtcatgc
     3721 cagcaagata atcgcatatt acctgttctt ttgactcttt atctattttt ttgtaagcgt
     3781 ggggaggcat ctgtcttggt tcttcataat aagcatcaaa aaggtctttt ataattcttg
     3841 ctgccttttc agccatcctg a
//
LOCUS       DAORYU010000137         3822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_15977_length_3822_cov_3.876029, whole genome shotgun sequence.
ACCESSION   DAORYU010000137 DAORYU010000000
VERSION     DAORYU010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15977_length_3822_cov_3.876029"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1704)
                     /locus_tag="PLW07_05095"
     CDS             complement(<1..1704)
                     /locus_tag="PLW07_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49935.1"
                     /translation="MRKVLLFLMFLSILNVGFVNAQNITFEKNGSAIIENSTISIRVS
                     ARSAGIEQWKYKITGFEMVDVLYGQTDYLKGHLLSEIWDPVTIDLIPAGRPDYGNFYV
                     SQGVSVNKEKNVSVIRQISEASYRLTRDIIVRNDLSVIEARITLVNLSGIPVGSSLRL
                     HNILSPGARGMYQNKNDILFLKTDKGILELDQSLNLENFYQLYGNDKFFNKNRENEPP
                     REWVNPRVLKTPILEENWAVWVNKGTGDGMVFVVDGDAFLGYYNCPGITIEPISKAFS
                     LKPGDIFQISVYVGSFTGIKNRKVSGANPLFIELEKLKVENGFLTGTVLPLWKGSLEI
                     ISDGKIIQKISVESQNSIEINSKLNQNEWLIKAIDSENHLIGEFENNGKISLSKPLFE
                     FPEKRKPKVMTKVFLAHNQKDSITSFLKKRDFTVYCSNDASEIEKNIAKEISKVLGAG
                     LTMTNPGGKILLVGNPQTNSMCKNIGIWKNSVDATWPGREKGAILFYSNYEETQQPVL
                     LITGSDEKGVVNAYEKFRNEFLKNIVPPSGFDFWVAGQDTKVFRYTRKFGNEPAETLK
                     LK"
     gene            complement(1704..>3822)
                     /locus_tag="PLW07_05100"
     CDS             complement(1704..>3822)
                     /locus_tag="PLW07_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49936.1"
                     /translation="GDKVEDSSLGILKELSAKWHIPLLTAADISDFLNNQYYKNDCKD
                     TAIILVGSPEENLVVRAHNKKYGFVDAYFPGNDKGIISVLENFLETGRPLIYVGGESP
                     CAAKKALEYLRNHFKPPMIRDLQVRIMPPEMRPRPWMKREKINNVELMVCKGETQPLH
                     LLLYSPGDIKNLEVMCDLKNSQKENIPYQVSYIPWSFAGVDKDGNIYPEGKQSTHFEG
                     HPLPRATFLPGSVAKEGTDIPFITYIANHDAMWPGVPKMIPSGRMVSLWITLAIEKNV
                     LPGFYEGAISIRWDRKEKKIPVKVEVVDITLPSHWKMDFFPMYSLDNYNENVFKLYLG
                     IDKKDEETYLNAIKKLGHLLYSHGATVANVSPYDMKISFYPDGTIKVDPAELNQVINA
                     YREGGFNGWFEMSVVPHYWEPMVEAISKFKNCSIEDARQIFFEKLKEWMRAMNIEGKM
                     ISRVSDEPGDADRWVEMASKLKNSGLLITVAHNQTKEEFVKKMIGTIDIWCPLWNRAI
                     TGWMGELLPDDEPSRFNRKFFHERKQKGEMIWNYTCATPYFSMTRLPTEIYFYFWDSY
                     NKKFDGVVYYGGGYWCHMWGGDDLPGVKGHIAHRDYYVYNVYGRNKKFDWVAGTTMFY
                     PDARIKEVISSQRFELVKQAQEDIKLFSLVRSNFGDEALDNLLSGCVSPRNHLDIPPE
                     DFCRVREKVIKFLLDHKNKQEAQ"
BASE COUNT         1084 a          827 c          585 g         1326 t
ORIGIN      
        1 ttttaactta agtgtctctg ctggttcatt cccgaatttt ctcgtgtaac gaaaaacttt
       61 tgtatcctgg cctgcaaccc agaagtcaaa accgcttggt ggaacaatgt ttttcaaaaa
      121 ttcatttcga aatttttcat atgcattaac cacccctttt tcatcgcttc ctgtaataag
      181 tagaaccggt tgctgtgttt cttcatagtt actatagaac aatattgctc ctttttctct
      241 tcctggccag gtggcatcta ctgaattttt ccatatacca atgtttttgc acattgaatt
      301 ggtctgagga tttccaacaa gcagaatctt tccaccagga ttagtcattg ttagtccagc
      361 accgagcact tttgatattt cctttgcaat atttttttca atctctgatg catcattaga
      421 acaataaaca gtaaagtccc tttttttaag aaaacttgtg atggagtcct tctgattgtg
      481 tgcaagaaaa acctttgtca taacttttgg ttttcgtttt tcaggaaact caaaaagagg
      541 tttgcttaaa ctgatttttc cattattttc aaattcgcct attagatgat tctcgctatc
      601 aattgctttt ataagccact cattctggtt taactttgaa tttatttcta tggagttttg
      661 tgattcaaca gagatttttt ggataatttt tccatcactg attatctcca aactgccttt
      721 ccacagaggt agtactgttc cggttagaaa accgttttca accttcaact tttctaattc
      781 aataaataaa ggatttgctc ctgacacttt tctattttta atacctgtaa atgatccaac
      841 atacacacta atttgaaata tgtctcctgg tttcaaagaa aacgctttcg atatgggctc
      901 tattgtgata cctgggcaat tataatagcc aagaaaagca tcaccatcca ccacaaaaac
      961 cattccgtct ccagttccct tattcaccca gaccgcccag ttttcttcaa ggataggcgt
     1021 tttaaggact ctggggttga cccattctct tggtggctca ttttctctat tcttgttaaa
     1081 aaatttgtca tttccataca attgataaaa attttcaaga tttagagatt ggtcaagttc
     1141 caatattcct ttatctgttt tcagaaaaag aatgtcattt ttattctggt acattcccct
     1201 tgcaccaggg ctcagtatgt tgtgcagacg taaagatgac ccaaccggaa tacctgaaag
     1261 attgactaag gtgatccttg cttctatcac agatagatcg tttcttacga taatatccct
     1321 tgttaatcta tagctggctt cgctaatctg tcttatcact gaaacatttt tctctttatt
     1381 tacactgaca ccctgactga cataaaaatt cccataatcc ggtcttcctg caggaataag
     1441 gtctattgta accgggtccc agatctcact taataaatga cctttcaaat aatctgtttg
     1501 tccataaaga acatccacca tttcaaatcc agtaatttta tatttccatt gttctattcc
     1561 tgcagaacga gcactgactc taatcgaaat agtagaattt tcaataattg ctgacccatt
     1621 tttttcaaat gtgatgtttt gagcattaac aaacccaaca ttaagaatcg atagaaacat
     1681 tagaaacaaa agcactttcc tcattattgc gcctcctgtt tgttcttgtg atctaagagg
     1741 aattttatta ccttttccct tactctacaa aaatcttctg gtggaatatc aagatgattt
     1801 ctgggtgaca cacaacctga tagtaggtta tctaatgctt cgtcaccaaa attactgcgt
     1861 accaggctaa ataatttaat atcttcctgt gcttgcttga ccagttcaaa tcgttgagag
     1921 gaaattacct ccttaatcct tgcatctgga taaaacatcg tggttccagc aacccagtca
     1981 aactttttgt tccttccata aacattgtat acatagtagt ccctgtgtgc gatatgacct
     2041 tttacacccg gcaggtcatc tccaccccac atatggcacc aatagccacc accatagtaa
     2101 acgaccccat caaatttctt attgtaactg tcccagaagt aaaagtatat ttcagttggt
     2161 aatctggtca tgctaaaata gggagttgcg caggtataat tccagatcat ttctcctttt
     2221 tgttttctct catgaaaaaa tttcctgtta aaccttgatg gctcatcatc cggtaacagt
     2281 tcgcccatcc atcctgtgat tgcacgattc cataaagggc accagatgtc aatagtgcca
     2341 atcattttct ttacaaattc ttctttcgtt tgattatgtg caacagtaat caacagtcct
     2401 gaatttttca gttttgaagc catttcaacc catcgatcgg catcacctgg ctcatcactg
     2461 actcttgata tcatttttcc ttctatgttc atagccctca tccattcttt taatttttca
     2521 aaaaatatct gcctggcatc ctcaatagaa caattcttaa actttgaaat tgcttcaacc
     2581 attggctccc agtaatgagg caccacactc atttcaaacc atccattaaa tccaccctct
     2641 ctgtaagcat ttattacctg attgagttca gcgggatcaa cctttattgt tccatcggga
     2701 taaaaactaa ttttcatatc ataaggagaa acatttgcaa ctgttgcgcc atgagaataa
     2761 agaagatgac cgagtttctt aatggcattt aaatatgttt cttcatcttt tttatcaatt
     2821 ccaagataaa gtttgaagac attttcatta taattatcca gactgtacat cgggaaaaag
     2881 tccatcttcc agtgagatgg cagcgtgata tcaacgactt caacctttac tggtattttc
     2941 ttttctttac gatcccatct aatgctgata gcaccttcgt aaaaacccgg caatacattt
     3001 ttttcaattg ccagagtaat ccataaagaa accatccttc ctgatggaat catttttggt
     3061 actcctggcc acatcgcatc atgatttgct atgtatgtaa taaagggtat gtcggtccct
     3121 tcctttgcca cagaaccagg caaaaatgtg gcccggggta aaggatgccc ttcaaaatgt
     3181 gtggattgtt ttccttcagg gtatatattt ccatctttat cgacccctgc aaatgaccat
     3241 gggatatatg atacttgata cgggatattt tctttttgtg agttcttcag gtcacacatc
     3301 acctctaaat tttttatgtc tcctggtgaa tataataata aatgcaatgg ttgggtttct
     3361 cctttacaaa ccattaactc gacattgtta attttttctc ttttcatcca gggcctgggt
     3421 ctcatttcag gtggcattat tcttacctgt aaatcgcgaa tcattggagg tttgaaatga
     3481 tttcttaaat attcaagagc ttttttcgca gcacagggac tttctccccc gacatatatc
     3541 aaaggtcttc ctgtttcaag aaagttttct aaaacagaaa ttattccttt gtcattaccg
     3601 ggaaaatagg cgtcaacaaa accatatttc ttattatgtg ccctgaccac cagattttct
     3661 tctggacttc caacaagaat tattgcggta tccttacagt catttttata atattgatta
     3721 ttcaaaaaat cgctaatatc tgcagcggtc aaaaggggta tatgccactt tgcagataat
     3781 tctttcaaaa taccaagtga tgaatcttcc actttatctc ca
//
LOCUS       DAORYU010000138         3789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_16191_length_3789_cov_3.725495, whole genome shotgun sequence.
ACCESSION   DAORYU010000138 DAORYU010000000
VERSION     DAORYU010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16191_length_3789_cov_3.725495"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="PLW07_05105"
     CDS             complement(<1..759)
                     /locus_tag="PLW07_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HOL49937.1"
                     /translation="MKAHMIIDFTRIIGKKNPYFFGSFIEHVHRCVYPGIFDPTSDFA
                     DKDGMRKDVLEEIRKLKPGTIRWPGGNFSSWYQWKDGIGPKEKRLTKISYADDIIREE
                     NHQFGTDEYIKFCRKTLVEPYITVNAGCGTAKEAVEWVEYCNYNGNSFYANLRRENGS
                     NTPFGVKFWEIGNEIYGDWQVGTKNAMQYCQVVNEFSKAMKRIDPTIKVVAVGMGKHD
                     PDWDRTILSHCWEYIDGISVHIYIGRHGYFDSFGQ"
     gene            1365..1592
                     /locus_tag="PLW07_05110"
     CDS             1365..1592
                     /locus_tag="PLW07_05110"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HOL49938.1"
                     /translation="MQEEKWKFLIESTEGESIVIESVLRANGIVVLKKSSGAQGYLRI
                     IGWMPGLNVKLYVPEDSYEQAVEIIKTIDEK"
     gene            complement(1609..2442)
                     /locus_tag="PLW07_05115"
     CDS             complement(1609..2442)
                     /locus_tag="PLW07_05115"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOL49939.1"
                     /translation="MKFSIQTIILPYNSLEEIGEIARTYGYDGIEWRVLPDSCLKDFG
                     PEAKNNLAVCSLKQSIPIIKKINAAFNLVVAGFSSYVEMDQLEDIKIIRDTGCELGCP
                     FFRIRGLYYRRDRNYHEIIEDGWRKFEKVEKLLEGYPMKAAIEIHHGFVVSSCAAAYH
                     FCSKFSPEHLGVIHDPGNQVVEGREDAKMGIEILGPYLIHVHFKNLIWEKKCVREDGT
                     IEWKPKSVPLNEGIIDWKDYIEQLKICGYNGFLSNEDMDTASGSIEQRLSQIKYLKQF
                     V"
     gene            complement(2455..>3789)
                     /locus_tag="PLW07_05120"
     CDS             complement(2455..>3789)
                     /locus_tag="PLW07_05120"
                     /inference="COORDINATES: protein motif:HMM:NF020121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HOL49940.1"
                     /translation="TDHPLILGGMMLPGSPDWFEMGLQRATTLMWTEDWMYGGIRSWG
                     NGLYQRLGFLCDILRNAASKHNQPLGFYNTMDGEDGIRMKGFIVIGHGVKIIDYFYYG
                     PTYSATENYWSDSISQYKGVAKVIRDIGKAEELVYPGQVPERNVAIIYSTTEEIWDQN
                     GTKGHEKQYLHIALAQQMYHADVLNEDLILERDLSQYKVIYFMDSHFPIAGLKKLKTY
                     VENGGTLVMMPGAGEKDEYDNTVNVITSLLASNIKTVSIEKPENTSINLNVSTGIESD
                     IPLTLSFKKAEISGAVKDQVLGKFLDGSPAILSTRVKKGSIIYYMFMPGHNFLSQVQQ
                     ANPEGYVNKFPEHARKLISVPCVMAGVKKQIEVSEGTFDASLLVSEKGTALVLVNLGR
                     DPLANVTFKVQAQGIKSVTSIENGNLQFKEENGYVHFSMPVNGLTDIVMLNK"
BASE COUNT         1043 a          813 c          679 g         1254 t
ORIGIN      
        1 ctgtccaaat gaatcaaagt atccatgcct tccaatgtat atgtgtacag aaatgccatc
       61 aatatattcc cagcagtgac tgagaatggt tctatcccag tcagggtcat gttttcccat
      121 tcccactgca acgaccttaa ttgtcgggtc aatccttttc attgcctttg aaaactcatt
      181 gacaacctgg caatactgca tggcattttt ggttccaacc tgccagtcgc cataaatttc
      241 atttccaatt tcccagaatt tcacaccaaa tggtgtgttg cttccatttt ctctgcgaag
      301 attggcatag aatgaattac cattgtaatt acagtattca acccattcta ctgcctcttt
      361 tgcagtacca cagccagcat tgactgtgat gtaaggttct acaagtgttt ttctgcaaaa
      421 cttgatatat tcgtctgttc caaactgatg gttttcttcc ctgataatgt catcagcata
      481 agaaattttt gttaatcttt tctcttttgg tcctatacca tccttccact gataccatga
      541 ggaaaaattc cctccaggcc atctaattgt gcctggcttc aattttctta tttcttccag
      601 gacatccttt ctcataccgt ctttgtcagc aaaatcagat gtcgggtcaa aaattcctgg
      661 atacacgcag cgatgaacat gttcaataaa agaaccaaaa aaatacggat ttttctttcc
      721 gataattctt gtaaaatcaa tgatcatgtg tgctttcatg cttcctccta tcccatctat
      781 gctacaacaa aatttattca aattcaacct gtgttcgtat taaattatga gacctgcgtg
      841 ggataggcaa agttctataa tttttcctca ttttcaaaag ggggattaag ggggatttca
      901 ataaatctcc cccgaccctt cttttataaa gaggggtgtg atttttctcc ctttacaaaa
      961 gggagacgca agagggattt catcaaatct ccatcccaac ctttgttttc ctctctcatt
     1021 tttttgtaaa atcccttttt ccgttctccc ccttttcaaa agggggattc agggggattt
     1081 caaaaatctc cctcgaccct tctaccactc ctgttctccc ctttgtaaaa ctcctcctac
     1141 tgttctcccc cttttcaaaa gggggattca gggggatttt gaaatctccc ccaacccctc
     1201 ttttataaag aggggtgtga taaggaaatt tataaagatg ggagtaaaaa agaacttaca
     1261 aaagcaggtg gatgggggga ttttgcaaat ttcatttctt gatatgagta tgtcattaaa
     1321 ttatgtagat tgattggatg gggtaattcg tgtaaaattc cttaatgcag gaagaaaaat
     1381 ggaagtttct tatcgaaagt actgaaggtg agagtattgt gattgagtca gtgctcaggg
     1441 caaacgggat tgttgtattg aagaaaagca gtggcgccca gggttatctt agaattattg
     1501 gatggatgcc aggactaaac gtgaaattgt atgtcccaga agattcctat gagcaggcag
     1561 ttgaaatcat aaaaacaata gacgaaaaat agttcccttt ttcaaatttc acacaaattg
     1621 tttcaggtac ttgatttgtg aaagccgttg ttcaatagaa cctgatgctg tatccatatc
     1681 ttcgtttgaa agaaacccgt tgtatccaca gattttcaac tgttcaatat agtccttcca
     1741 gtcaatgatt ccttcattca gtggtacact tttgggtttc cattcaatgg ttccatcttc
     1801 tcttacacac tttttctccc atatcaaatt tttgaaatga acatggatca gatatggtcc
     1861 gaggatttca attcccattt ttgcgtcttc cctgccttca acaacctggt tgccagggtc
     1921 atggatgaca ccaagatgtt cagggctgaa ttttgagcaa aaatgatatg ctgccgcaca
     1981 ggaacttacg acaaaaccat gatgtatttc aatggctgct ttcattggat aaccttcaag
     2041 aagtttctca accttttcaa attttctcca tccatcttca ataatttcat ggtagttacg
     2101 gtctcttctg taataaagtc ccctgattct gaaaaatgga cagccaagtt cacatcctgt
     2161 gtccctgatg attttgatat cttccaactg gtccatttca acataggaag aaaaaccagc
     2221 aacaaccaga ttgaatgctg cattaatttt tttgataatg gggatacttt gtttcaaact
     2281 gcacactgca agattgtttt ttgcttcagg tccaaagtcc ttgagacagg aatcaggtaa
     2341 aaccctccat tcaattccat cataaccata tgtgcgcgca atttctccaa tttcttccaa
     2401 actgttgtat ggaagaatga ttgtttgaat actgaatttc attgttgcct ccagttattt
     2461 gttcagcatg acaatatctg ttaatccatt gacaggcatt gaaaaatgta cataaccatt
     2521 ttcttctttg aattgaagat taccattttc aattgatgtt actgatttga taccctgtgc
     2581 ctggacttta aaagtgacat ttgccaatgg gtctcttcca agattgacca gtaccagcgc
     2641 tgtgcctttt tctgacacaa gtaaactggc atcaaaagtg ccttctgata cttcaatctg
     2701 ctttttgaca ccagccatta cacatggaac actgataagt tttcttgcat gttcagggaa
     2761 tttgttgaca tatccctcgg gatttgcctg ttgaacctgc gataaaaagt tgtgtccagg
     2821 cataaacatg tagtaaataa tgctaccctt ttttaccctg gtactgagaa ttgctggaga
     2881 gccatctaaa aacttaccca gaacctggtc ttttacagcg cctgaaattt ctgccttttt
     2941 gaatgaaagg gtgagaggga tatcgctctc aatgcctgta cttacgttca gattgatgct
     3001 ggtgttttcg ggtttttcaa tggatactgt ctttatgttt gaggcaagaa gtgatgtaat
     3061 cacattgaca gtattgtcat attcatcctt ttcacctgca cctggcatca ttaccagtgt
     3121 tccaccattt tcaacatagg tttttaactt tttcaatcca gcaatcggaa aatgtgaatc
     3181 cataaaatag atgaccttat attgagacag atctctttcc aggattaaat cttcattcaa
     3241 tacatctgcg tgatacattt gctgggcaag agcaatatgg agatattgtt tttcatgccc
     3301 ttttgttcca ttttggtccc agatttcttc tgtggtgctg taaatgattg ccacatttct
     3361 ttctggcacc tgtccaggat atacaagttc ttctgctttg ccaatgtccc tgatgacctt
     3421 tgcaacgccc ttatactgcg aaatactatc tgaccagtag ttttctgtgg ctgaatatgt
     3481 gggtccataa taaaaatagt caatgatttt tactccatgg ccaattacga taaatccttt
     3541 cattctgatg ccatcttctc catccattgt attgtaaaaa cccagtggtt gattgtgttt
     3601 gcttgcggca tttcgtaaaa tgtcgcaaag gaaaccaagt cgctggtaaa gaccatttcc
     3661 ccatgacctg attccaccat acatccaatc ctctgtccac atcagggtag ttgccctctg
     3721 taaacccatt tcaaaccagt cagggctacc tggaagcatc ataccaccaa gaatcagggg
     3781 atggtctgt
//
LOCUS       DAORYU010000139         3777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_16246_length_3777_cov_5.532241, whole genome shotgun sequence.
ACCESSION   DAORYU010000139 DAORYU010000000
VERSION     DAORYU010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16246_length_3777_cov_5.532241"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PLW07_05125"
     CDS             complement(<1..214)
                     /locus_tag="PLW07_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49941.1"
                     /translation="MNNNSCTSNFFRIVFVISSLGCGGAERVVTNIANYLSLLGHYVS
                     IITFDSPFNKPFFPLEKSVELIQCGLL"
     gene            complement(204..1184)
                     /locus_tag="PLW07_05130"
     CDS             complement(204..1184)
                     /locus_tag="PLW07_05130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOL49942.1"
                     /translation="MTELNTFILKMAKVSVIIPVYNASRFIGETIKSVLSQTYKDFEI
                     IVVDDGSTDNSADIVKSFGDKKIILIKQKNKGVSAARNTGIKASTGKFIALLDHDDLW
                     LPEKLEKQIPLLEKNPDVALVYSDVCYIDARGNPVSWMLKQFEPHRGYVLKELFITDF
                     VPCLTAVFRKDVIEKIGFFNERYRYAEEYELFLRMARFFQFDFVDEVLACYRVHQNNL
                     SRNIIQSYLENIEILEHFYHSVPEVKKFEYVARKRIANMYFITGRRFQVEERNRTQAI
                     KMFRKSLNYNSTSMKSILGLLTAFFNIPVPRRHRILKFFTPKNIFDAHEQ"
     gene            complement(1156..2259)
                     /locus_tag="PLW07_05135"
     CDS             complement(1156..2259)
                     /locus_tag="PLW07_05135"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOL49943.1"
                     /translation="MIPVAKPFVGEEELEEVRKVFQSGWLGMGAWVKQFEDQLKNFIG
                     TKYVIATNTGTSAIHLALVSCGIKSGDEVIVPSLTFAATIQAIVATGADVVFCDVQPD
                     TLNIDPKDIKKRITSKTKAIVPVHYRGYPCDMEEIEKIAEENGLRIIEDAAHAFGSKY
                     RGKKIGSFGDITCFSFDPIKIITCGEGGAIITVDENVAKLVEKGRILGISRDTWSRYK
                     HERSWFYDVETAGFRYHMSNINAAIGIVQLKKFDEFLKRRLAIVRKYDENLKDIKCIT
                     LLKTDYESMAPFCYIIRAEKRDDLMAYLKENGVDTGIHYIPNHLQSYFSKYKVSLSVT
                     EKIWNEILTLPLYYGLEDEQVLMICDRIKHFYS"
     gene            complement(2268..3104)
                     /locus_tag="PLW07_05140"
     CDS             complement(2268..3104)
                     /locus_tag="PLW07_05140"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOL49944.1"
                     /translation="MFPRYVFAGYSKSKHLKDIKQVMGNYDMMKAPSEDYFKERYLEM
                     INEIINQNFRHGISLRILDAGCGQGRIAIELAKMGHTVDAMDYVEETIEKGKKYAEEM
                     LPNASINWKTGKIPDDLSFYQDEAYNMVLCLEVLYMMSHDECCKSLQELAKKVKKGGM
                     LMISVRPRYYYLGYYLINRDFLRLQHVAKNLDFSELGQYLSWTDKSLMEEIFRNLGFE
                     NINAKGLGILTGIQGDPTALFCRPELLSENEKRIVAEVENTYSSSYPEAGRYIVFWAT
                     KR"
     gene            complement(3140..>3777)
                     /locus_tag="PLW07_05145"
     CDS             complement(3140..>3777)
                     /locus_tag="PLW07_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49945.1"
                     /translation="TRDILIKNGFDPFRVISGPSLRYQYLFDFKNHCSGPAENKNILV
                     ICPLAIDSAVELLLKSIEAIKNLQNLYDNVYVKIHPMMNSLELLKAAEIKKLPEKWKF
                     VSGTMEQWLKNSSCAVCMDSSAVIEAVIAELPVIIVGRETNFTMNPLPWLQEPEFQPV
                     FLVEQLQKRILECLEITDERKKYLKARSEEMLNRLSQINEENLMVFITSKQ"
BASE COUNT         1158 a          790 c          537 g         1292 t
ORIGIN      
        1 caagtaaacc acattgaatc agttcaaccg acttttccag cgggaaaaac ggtttattaa
       61 aaggactgtc aaaagtaata atactcacat aatgccccag taaggataaa taatttgcaa
      121 tatttgtaac aaccctttct gcacccccac atccaagact ggatattacg aaaactatcc
      181 tgaagaagtt acttgtgcat gaattattgt tcatgagcat caaaaatgtt tttaggggta
      241 aaaaacttca gaatcctgtg tcttctcggg actggaatat taaaaaacgc agttaaaaga
      301 ccgagtatgg atttcattga ggtgctgtta taattcaacg attttctaaa catttttatt
      361 gcctgggttc tgtttctttc ttcaacctga aatcgccttc cagtaataaa atacatgttt
      421 gcaattcttt tccttgcaac atattcaaat tttttcactt ctggaacaga gtgataaaaa
      481 tgctcaagta tttcaatatt ttcgagataa ctttgaatta tgtttctact gagattattc
      541 tgatggaccc tgtagcaggc aagaacctca tcaacaaaat caaattggaa aaatcttgcc
      601 attctcaaaa ataactcata ctcttcagca tatctgtatc tttcattaaa aaaacctatt
      661 ttctcaatga catcctttct aaaaacagca gtaagacaag gtacaaaatc agtgatgaaa
      721 agttccttca aaacatagcc gcgatgcggt tcaaattgtt ttaacatcca tgatactgga
      781 ttacctctgg catctatata acatacatct gaatacacaa gagccacatc aggatttttt
      841 tctaataaag gtatctgctt ttcaagtttt tctggaagcc acaaatcatc atggtcaagc
      901 aaagcgataa attttcctgt tgatgctttt attccagtat tacgggcagc tgaaaccccc
      961 ttattttttt gtttaattaa aataattttc ttatctccaa aacttttcac aatatctgcc
     1021 gaattgtcag ttgaaccatc atccacaaca ataatttcaa aatccttata tgtttgagac
     1081 aatacgcttt ttatcgtctc accaataaat ctggatgcat tataaaccgg gatgatgacg
     1141 cttaccttag ccattttaag aataaaagtg tttaattctg tcacatatca tcaatacctg
     1201 ctcgtcttct aaaccgtaat aaagcggcaa tgtaagtatt tcattccaga ttttttctgt
     1261 gacagaaagt gataccttgt attttgaaaa atacgattga agatggttag gaatatagtg
     1321 aatacctgta tcaaccccat tttctttaag gtatgccatc aaatcgtctc ttttttctgc
     1381 tcttatgata tagcagaatg gagccattga ctcatagtca gttttaagaa gtgttataca
     1441 tttgatgtcc ttcaaatttt catcgtactt tctgacaata gccagccttc ttttcaaaaa
     1501 ttcatcaaac ttctttaact gaacaatacc aattgctgcg ttgatattgc tcatatgata
     1561 tctgaaccca gctgtctcaa catcgtaaaa ccagcttctt tcgtgtttat accttgacca
     1621 tgtatccctg cttataccaa gaattcttcc cttttcaacc agtttagcca cattttcgtc
     1681 aactgttatt attgcaccac cttcgccaca ggtaatgatt tttattgggt caaaactgaa
     1741 gcaggtaata tcaccgaaag aaccaatttt ttttcctctg tactttgaac cgaatgcatg
     1801 cgcagcgtcc tcaataattc ttaaaccatt ttcttctgca attttttcaa tttcttccat
     1861 atcacaggga tatcccctgt aatgaacagg cactattgct tttgtttttg aagtgattct
     1921 tttttttata tcctttgggt caatattcaa tgtatcgggc tgcacatcac agaaaacaac
     1981 gtctgcacct gttgccacaa ttgcctgtat ggtcgcagca aacgttaaag aaggaacaat
     2041 tacttcatct ccgctcttta taccacacga tacaagtgca agatgaattg cagaggttcc
     2101 agtatttgtg gcaataacgt atttggttcc aatgaaattt ttcaactggt cttcaaactg
     2161 tttcacccat gctcccattc ctaaccatcc agattgaaaa acctttctca cttcttcaag
     2221 ttcttcttca ccaacaaaag gttttgcaac aggtatcatg tgttctccta tcgttttgtt
     2281 gcccaaaata caatgtacct acctgcttca ggataactgc tgctgtaagt attttctact
     2341 tcagcaacaa tccttttttc attttctgaa agtagttcag gcctacaaaa aagagctgtt
     2401 ggatctccct gtatccctgt aagtatcccc agtccttttg cgtttatatt ttcaaaaccc
     2461 aaatttctga aaatctcttc catcagagat ttatcagtcc aggaaagata ttgtccgagt
     2521 tctgaaaaat ccagattctt tgcaacatgt tgcagtcgca aaaaatccct gtttatgaga
     2581 tagtagccca gataataata cctcggtctc acagaaatca ttaacattcc acctttttta
     2641 accttctttg caagttcctg taaactcttg caacattcat catgggacat catgtaaaga
     2701 acctccagac acaaaaccat gttatacgct tcatcttgat aaaaagaaag gtcatctggg
     2761 atttttcctg ttttccagtt aatagatgca ttgggcaaca tctcttctgc gtattttttc
     2821 cctttttcaa ttgtttcttc aacatagtcc attgcatcaa cagtatgccc cattttagca
     2881 agttcaattg ctattcttcc ctgcccgcaa ccggcatcaa gtattctcaa tgaaattcca
     2941 tgtctgaaat tctgattgat gatttcattg atcatttcaa gatatctttc cttaaaataa
     3001 tcctcagaag gtgctttcat catatcataa ttacccatta cttgtttaat atccttcaaa
     3061 tgttttgact tgctatatcc agcaaatacg taacgcggaa acattttcaa ccactttaaa
     3121 aatccagtaa taaattgttt cattgttttg aagtgataaa aaccattagg ttttcttcat
     3181 taatctgaga taacctgttt aacatttcct cacttcttgc tttcaaatat ttttttcttt
     3241 catctgtgat ttcaaggcat tcaagaattc ttttttgcaa ttgttccaca agaaatactg
     3301 gttgaaattc tggttcctgt agccagggca aaggattcat tgtaaaattc gtttcacgtc
     3361 ccacaatgat taccggtaat tctgcaataa ctgcctcaat gactgcagaa ctatccatgc
     3421 acacagcaca tgaactattt ttaagccatt gttccatagt tcccgacaca aatttccatt
     3481 tttcgggtaa ctttttaatt tctgctgcct ttaataattc taaagaattc atcattggat
     3541 gaattttgac ataaacattg tcatacaaat tctgtaaatt ctttattgct tcaatacttt
     3601 tcaacagtaa ttccacagca gaatcaattg cgagcgggca gataactaaa atatttttat
     3661 tttctgctgg accagaacag tgatttttaa aatcaaataa atattgatac cttaatgaag
     3721 gacctgaaat aacacgaaat gggtcaaaac catttttaat caaaatatca cgcgtcc
//
LOCUS       DAORYU010000140         3767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_16305_length_3767_cov_3.632543, whole genome shotgun sequence.
ACCESSION   DAORYU010000140 DAORYU010000000
VERSION     DAORYU010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16305_length_3767_cov_3.632543"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..1227
                     /locus_tag="PLW07_05150"
     CDS             97..1227
                     /locus_tag="PLW07_05150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HOL49946.1"
                     /translation="MKQFSLLIKPSSSTCNSDCEYCFYKNLSVYKNRQSTFMTHQVLE
                     TLIAKYLSTQQNQYIFSWQGGEPLLLGLDFYKTVVQLQKTLAKAGSIIGNGIQTNGYL
                     IDDAYAEFFSVSNFLVGISCDGPEQLHNTFRKIPDNSNAFNLVIKSIKTLQKHDVAFN
                     TLTVVTSQHAGKGKLVYQFLKDTGSDYHQYIPCVEYDKNGKKLPWTIEGIEWGKFLIE
                     IFQQWLLDSQKVSIRLFDSIILVLLGQKPGICQMEKNCCQYFVIEHNGDVFPCDFFVK
                     NHTLLGNITNDTWENLLNSEKYRGFGARKSLWHSDCDRCYYLKFCAGDCQKHRIGKNK
                     SERTKSVLCDGWKLFYKETLTEFKKLAQLYKQHYLTRMSIEF"
     gene            complement(1197..2090)
                     /locus_tag="PLW07_05155"
     CDS             complement(1197..2090)
                     /locus_tag="PLW07_05155"
                     /inference="COORDINATES: protein motif:HMM:NF013296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOL49947.1"
                     /translation="MNIGKIMRAAYDNHVIIPAFNVAYLPMVKPISDALVESRTIGII
                     EVSRPDIENFGAKSIRHVFQQYTTDADQKFTFLHLDHVPVIDEHFLPVNWLAMIDEAI
                     ELGYDSVMIDGSRLPFADNVSITKKVVEMAHEKNICVEAELGSVMGHEPFPLPPYEEI
                     FEKKIGFTDPEAAQKFVKMTGVDWLSVSAGSIHGAISGAAKHEKKIRARLDIEHLEKI
                     KNATGIPLVLHGGSGIDEECFKKAIKTGIAKINIGTEIRQAYEEGIKIDEKTACENVK
                     AVVKKLIETYQVQNSIDILVK"
     gene            complement(2107..3297)
                     /locus_tag="PLW07_05160"
     CDS             complement(2107..3297)
                     /locus_tag="PLW07_05160"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOL49948.1"
                     /translation="MLNIGIVGAGNRGIRCYGIHIMKEKKDIARIVAISDPDEKRLAV
                     AKQVLHLDNDDIFTDTRKMLDKKDLNAVIITSPDYTHRDIAIAAFERGLDVLCEKPLA
                     LTVDDCDEILKAQEKSGKILCVGFVLRYNNFYRKMYEIVNVEKKIGKLIVAGGFDSVS
                     TGSQYFFHGWWRLRKNSGGLLVQKATHSVDILNWIIGSKATRVYGEGGLAVFGGKEPD
                     DKVCSSCSKRFNCPEALLEKNIAWDYGEASITMEVEDKCVFAKEIDVYDHEILTINYE
                     NNVKAFFVESQFTHDYKREFWFIGDKGRLYGIDPYIVASQDNKKPYMEIVYRHTREKE
                     IIPVTLAPGGHGGGDPGLIDDFLNGCITRKNVLADGIAGRESIAICAGGEKSIETGKI
                     IWLR"
     gene            complement(3320..>3767)
                     /locus_tag="PLW07_05165"
     CDS             complement(3320..>3767)
                     /locus_tag="PLW07_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010586472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49949.1"
                     /translation="KEAEKARKEYERLKKEIENNSEIMKQSRWYRDITYHFRKYRWFQ
                     RCVERAKNKKNSSVQIEVHVVRVGDIVFVTNPFELFLDYGIRIKAQSKAIQSFIVQLA
                     GGGTYLPTERAVKGKSYGAIGPSCLVGPEGGKQLVEKTLEIIHNLF"
BASE COUNT         1106 a          812 c          612 g         1237 t
ORIGIN      
        1 agggtattat agtgctgctg gtcacttgta ataaatagta tatttggcat ctttttcatt
       61 ttttctccat aagcggctga gttataatac attattatga aacaattttc attgcttatc
      121 aagccatcgt catcaacatg taattcagat tgcgaatact gtttttataa gaatctttct
      181 gtctacaaga ataggcagtc cacattcatg acccaccagg tacttgaaac actgattgca
      241 aagtacctat caacacagca aaatcaatac attttctcct ggcagggagg agaaccccta
      301 cttctgggac ttgattttta taaaaccgtt gtgcaacttc aaaaaacact tgcaaaagcg
      361 ggctctatca ttggcaatgg cattcaaaca aatggttatc tcattgatga cgcttatgct
      421 gagttctttt cagtttcaaa tttccttgtc ggcatcagtt gtgatggtcc agaacagctg
      481 cacaacacat tcagaaaaat ccctgacaac agcaatgctt tcaatcttgt tatcaaaagt
      541 attaaaactt tgcaaaagca cgatgttgcc tttaacaccc tgacagttgt cacttctcaa
      601 catgcgggaa aaggaaaact cgtatatcaa tttttgaaag acactggttc tgactatcac
      661 cagtatattc cctgcgttga atatgataaa aatggtaaaa aactgccatg gacaatagaa
      721 gggatagaat ggggaaaatt tttgattgaa atcttccagc agtggttgct tgacagtcaa
      781 aaagtttcaa tacggctttt tgattcaatc attcttgttt tactgggaca gaaacccggt
      841 atctgtcaga tggaaaaaaa ctgctgtcag tattttgtca ttgaacacaa tggagatgtt
      901 tttccatgtg atttctttgt aaaaaatcac acactgcttg gaaacattac aaatgatacg
      961 tgggaaaacc tgttgaattc tgaaaaatac aggggcttcg gagcaagaaa atcgctgtgg
     1021 cattcagatt gtgatagatg ctattacctg aagttttgcg cgggtgattg ccagaagcac
     1081 agaatcggga aaaacaaatc agaaagaacg aaaagtgtcc tttgtgatgg atggaaattg
     1141 ttttataaag aaacattaac tgaattcaaa aaacttgctc aattgtacaa gcagcattat
     1201 ttgacaagaa tgtcaataga attttgaacc tgatatgtct caatcagttt ttttacaact
     1261 gccttcacat tttcacaggc tgtcttttca tcaattttta ttccttcctc atatgcctgt
     1321 cttatttctg tccctatatt aattttggca atgcctgttt ttattgcctt tttgaagcat
     1381 tcttcatcta tacctgatcc tccatgaaga accagcggaa ttccagtagc attttttatt
     1441 ttctcaagat gctcaatgtc aagacgtgcc ctgattttct tttcatgttt tgcagcgcca
     1501 gaaattgctc catgaattga accagcagaa actgaaagcc aatcaacccc tgtcatcttt
     1561 acaaatttct gcgctgcttc agggtcagtg aaaccgattt ttttttcaaa gatttcttcg
     1621 tatggtggga gcggaaatgg ttcatgtccc atgacagaac caagttctgc ctcaacacat
     1681 atattttttt catgcgccat ttcaacaact ttttttgtaa tactgacatt gtctgcaaat
     1741 ggaagccgtg aaccatctat cattacagaa tcgtatccaa gttcaatggc ttcatcaatc
     1801 attgcaagcc agttcacagg taaaaaatgt tcatcaatta caggtacatg gtcaagatgc
     1861 aaaaatgtaa atttctgatc agcgtcagtt gtgtactgct ggaaaacatg tctgatgctt
     1921 ttcgcaccaa aattttctat atcaggcctt gaaacttcaa taataccaat tgttctggat
     1981 tcaaccagtg catcagaaat tggctttacc attggaagat acgcaacatt gaatgcagga
     2041 ataataacat gattgtcgta tgctgccctc attatttttc caatgttcat atacgggttc
     2101 atttttttac ctcaaccaga taatcttgcc tgtttcaatt gatttttcgc cacctgcgca
     2161 gatggcaata ctttcccttc cagcaatccc atcagcaaga acattttttc gagtaataca
     2221 gccattcaaa aaatcatcaa tcaatccagg gtctccacca ccatgaccac ctggagcaag
     2281 tgtaacagga ataatttctt tttctcgtgt atgcctgtaa acgatttcca tgtatggttt
     2341 tttattatcc tgtgaagcaa cgatatacgg atcaattcca tacaatcttc ctttatcccc
     2401 gataaaccag aattcacgtt tgtaatcatg cgtgaactgg ctttcaacaa aaaatgcttt
     2461 tacattattc tcatagttga ttgttaaaat ttcatggtca tacacatcaa tttcttttgc
     2521 aaaaacacat ttgtcttcaa cttccatggt aatactggct tcaccataat cccaggcaat
     2581 gtttttttca agcaatgcct cagggcaatt gaatcttttt gaacaactgg aacatacctt
     2641 atcatctggc tcttttccac caaacactgc cagaccacct tctccataaa cccttgtcgc
     2701 cttactgcca ataatccagt tgagaatatc aacagaatgg gttgccttct gaacaagaag
     2761 gccaccgcta ttttttctca accgccacca gccatggaag aagtactggg aacctgttga
     2821 tactgaatca aatccacctg ccacaatgag ttttcctatt tttttctcaa cattaacaat
     2881 ctcatacatt ttcctgtaaa agttgttata tctaaggaca aagccaacac acagaatttt
     2941 tcctgatttc tcctgggctt tcaaaatttc atcacagtca tccactgtta atgccaatgg
     3001 tttttcacac agaacatcaa gccctctttc aaaagcagca attgcaatat ccctgtgcgt
     3061 gtagtcaggg ctggtaataa ttactgcatt caaatccttt ttatcaagca tttttctggt
     3121 atcagtaaat atatcatcat tatccagatg aagaacctgc tttgcgacag caagccgttt
     3181 ttcatcagga tctgagatcg caacaatcct tgcgatatcc tttttttctt tcataatatg
     3241 tatgccatag catctgattc cgcgattgcc agcaccaaca attcctatgt tcaacattgt
     3301 tcctccagag tcaaatgttc taaaagagat tgtggattat ttcaagggtt ttttcaacaa
     3361 gttgttttcc accctcagga ccaacaaggc aggatggtcc tattgcacca taactttttc
     3421 ctttcactgc tctttctgtg ggaagatagg ttccgcctcc tgccagttgc acaatgaacg
     3481 attgaatcgc tttactctgt gcctttatcc tgattccata atcaagaaat aattcaaatg
     3541 gattggtgac aaacacaatg tcgcccaccc tcacaacgtg tacttcaatt tgaacactgc
     3601 tgtttttttt gtttttagct ctctccacgc atctttgaaa ccacctgtat tttctaaaat
     3661 gatatgtaat atccctgtac catctcgact gcttcattat ttcagaatta ttttcaattt
     3721 cttttttcaa cctttcgtat tctttacgag ctttttctgc ttctttt
//
LOCUS       DAORYU010000141         3761 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_16346_length_3761_cov_4.096330, whole genome shotgun sequence.
ACCESSION   DAORYU010000141 DAORYU010000000
VERSION     DAORYU010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16346_length_3761_cov_4.096330"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="PLW07_05170"
     CDS             <1..472
                     /locus_tag="PLW07_05170"
                     /inference="COORDINATES: protein motif:HMM:NF024757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta galactosidase jelly roll domain-containing
                     protein"
                     /protein_id="HOL49950.1"
                     /translation="ESGIMIAPVPIEAKFSRDPFNEGIVGEWYLPEFNDKNWEMKNTF
                     YLWDQQDKPLDAIGHDYDGYGWYRFWANIPSNMKEKSVHFYCGGVINEGWVWVNGEYA
                     GHKSHELWWNGKHDFDLDITKFVKYGTKNLITIRVWNSQEVGGLYRRGFVWSPK"
     gene            472..1506
                     /locus_tag="PLW07_05175"
     CDS             472..1506
                     /locus_tag="PLW07_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015615307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOL49951.1"
                     /translation="MEYTTLGRTGLRVSRVGFGALPIQRTELSRAIKILQSAFEYGIN
                     IFDTARGYTDSEKKLGIAFKGKRDKIILATKVHGQNKQEVISLLRESLKQLKTDSVDI
                     LQLHNPGKLPNPDDESSAYAGLKEAQKKKMTRFIGITAHKLTNAKSAIESGLYDVVQY
                     PFSMLSSKEELELIKLAKKHNIGLLAMKPLCGGILVDIKVSFAFLWQFENLVPIWGIQ
                     RMKELKQMVNLSKNPPEIDAELGRKISEEKRTLGSYFCRGCGYCLPCPADIPIPMAAR
                     MKYLLRRAPYRKFLTVQWQKNMLKIENCKNCGVCKSRCPYELNPSELMKEMLADYIDF
                     AKTRGVTIKI"
     gene            1516..1992
                     /locus_tag="PLW07_05180"
     CDS             1516..1992
                     /locus_tag="PLW07_05180"
                     /inference="COORDINATES: protein motif:HMM:NF024126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HOL49952.1"
                     /translation="MKHDYSHILLDHFIKRMRKITKERDEILKQIKTVKDAKLYQQQV
                     KKIIKQAFSPLPEKTPLNPRITGIEKRKKYRIEKIIFESRPECFVTANLYIPEHFSAP
                     FPAVIGTCGHSATGKAEPRYQEFCQRLVQNGFLVFIYDPFNQGERDQYIHYQRTPF"
     gene            2019..3407
                     /locus_tag="PLW07_05185"
     CDS             2019..3407
                     /locus_tag="PLW07_05185"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOL49953.1"
                     /translation="MMGKQLQLIGEFFGSWRLWDGIRALDYVLTRHETEKNFIGITGN
                     SGGGTMTTWLWGNEPRFTAAAPSCFITPFRYNIENEMPQDIEQCPPGILGHNIDIADF
                     FISRAPEPLILLGQKYDYFDIRGFKEICDQVKKIYKIFDAEDNFSFFIGSNPHGYYPD
                     AQKNMVSFFCKIAGKKDINSEPEINIEKSETLFATEKGNVVHAGSKPVYVIIGKKAED
                     IERTRPQLPEDKLKEKIRKLLKIPEIKETPHYRVLRPQVIKKSVIARYAVESEQEIWV
                     ILKKKADRPDFIYNLQIEDEINLCLPHISSEDEILKKRKFLPGMVEYFVDVRGLGESM
                     PEEKKNFFRPYGYDFMMDMCYAMFGETYFGKRIFDVLSVIKLLEEKGCKTINLYGNHQ
                     GALIGLFVAFLSVPVKKVYLKNLPESFCSLCKKLSTELPSANFPKGIMKITDIPEILN
                     YLKKTKEAVVIR"
     gene            complement(3445..>3761)
                     /locus_tag="PLW07_05190"
     CDS             complement(3445..>3761)
                     /locus_tag="PLW07_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49954.1"
                     /translation="KYVAQFEPWFSDFNVIPKSMHDEFADAVVRNNKIVEINLHAIFL
                     HPSYPENFKMQYLEYLSFLKSKGVKFSIGTDCHQSYNLDLEKAASMIEKAGITEQDIW
                     SI"
BASE COUNT         1326 a          650 c          744 g         1041 t
ORIGIN      
        1 agaatcaggc atcatgattg cacctgtacc tattgaggcg aaattttcac gcgatccatt
       61 taatgaaggt attgttggtg agtggtattt gccagaattt aatgacaaaa actgggaaat
      121 gaaaaacaca ttttatttat gggaccagca ggacaaaccc cttgacgcta taggacatga
      181 ttatgatggc tatgggtggt atagattctg ggcaaatatt ccatcaaaca tgaaggaaaa
      241 atcagtacat ttttactgtg gtggcgtcat caatgaagga tgggtatggg taaacggaga
      301 atatgctggc cataaatcac atgaattatg gtggaatggt aaacatgatt ttgaccttga
      361 tattacaaag tttgtaaaat acggtacaaa aaatctcatt acgatcaggg tctggaatag
      421 tcaggaagtc ggtggcttat accgcagggg ttttgtctgg tctccaaaat aatggaatat
      481 acaactcttg gtagaacagg tctaagggta agccgggttg gatttggtgc attacctatt
      541 cagagaacag aattaagcag agccattaaa attctacaat ctgcctttga gtacggtatt
      601 aatattttcg acacagcaag gggttatact gatagtgaaa aaaaacttgg tatcgcattc
      661 aaaggcaaaa gggataaaat cattcttgca acaaaggtgc atggacaaaa taaacaggaa
      721 gtaatctcac ttcttcggga aagcctaaaa caacttaaaa ctgactctgt agatatactt
      781 caacttcata atccgggaaa attgccaaac cctgatgatg aaagttctgc atacgctggc
      841 ttgaaggaag cacagaagaa aaaaatgaca agatttatcg gaattactgc acacaaacta
      901 acaaatgcaa aatctgcaat agaatcaggc ctttatgacg ttgtacaata tccattctca
      961 atgctttctt caaaagagga actggagttg attaaacttg caaagaaaca caatatcggg
     1021 ctacttgcaa tgaagccact gtgtggtggg atacttgtgg atattaaagt atcatttgcc
     1081 ttcctgtggc agtttgaaaa ccttgttcca atctggggta tccagagaat gaaagaattg
     1141 aagcaaatgg tcaatctttc aaaaaaccca cctgaaatag atgctgaatt aggacgaaag
     1201 atatcagaag aaaaaagaac gcttggctca tatttttgca ggggctgcgg ctattgtctg
     1261 ccctgccctg ctgatattcc aattccgatg gcagcaagga tgaagtatct gttaagaagg
     1321 gctccttaca gaaaattttt aacagtccag tggcaaaaaa acatgttgaa aatagaaaac
     1381 tgcaaaaatt gtggggtatg taaatccaga tgtccttatg aacttaatcc gtccgaatta
     1441 atgaaagaaa tgcttgctga ttacattgac tttgcgaaaa ccagaggagt gactattaaa
     1501 atataggaga aacatatgaa acatgactat tcccatatcc tgcttgacca ttttattaag
     1561 agaatgagaa aaatcacaaa agaaagagat gaaatcttaa aacagataaa aacagtcaaa
     1621 gacgcaaaat tatatcaaca acaagtaaaa aagataatca aacaagcatt ttctcctttg
     1681 ccagaaaaaa cacctttgaa tcccagaatc actggaattg aaaaaagaaa aaaatatcgc
     1741 attgagaaaa tcatttttga aagcagacca gaatgttttg tcactgccaa tctctatatt
     1801 cctgaacatt tcagcgctcc atttccagca gtaatcggaa cctgtggtca ttcagcaact
     1861 ggtaaggcag aaccaagata tcaggaattc tgccagcgtc ttgttcaaaa tggttttctt
     1921 gtgtttattt atgacccatt caatcagggt gaaagagacc aatacataca ctaccaaaga
     1981 actccatttt aagacgcagt tgctgcgcgg cacataacat gatgggcaag caactgcaac
     2041 tgatcggtga gtttttcggt tcgtggcgat tgtgggatgg aataagagcg cttgattacg
     2101 ttttaactag acatgaaaca gagaaaaatt tcataggaat tactggcaat tcaggtggcg
     2161 gaaccatgac cacgtggctg tggggaaatg aaccacgctt tactgctgct gccccaagtt
     2221 gttttattac cccattcaga tacaacatcg aaaacgaaat gcctcaggat atagaacaat
     2281 gtccgccagg gatacttgga cacaacattg acattgctga cttttttata tcccgtgcac
     2341 ctgaaccact aatacttctg gggcaaaaat atgattactt tgatatcagg gggtttaagg
     2401 aaatatgtga ccaggttaaa aaaatttata aaatcttcga tgccgaggat aatttttcat
     2461 ttttcattgg tagtaatccc cacggctatt atccggatgc acaaaaaaac atggtttctt
     2521 tcttctgtaa aatcgcaggg aaaaaggata ttaattcaga gccagaaata aacattgaaa
     2581 aatcagaaac actatttgcg acagaaaaag gcaatgttgt tcacgctggt tcaaaaccag
     2641 tgtatgtgat aataggaaaa aaggcagaag atattgaacg aacaagacca caattaccag
     2701 aagataaatt aaaagaaaaa attaggaaac ttttgaaaat tcctgaaata aaagaaaccc
     2761 cgcattacag agttctcaga cctcaagtga taaagaagtc tgttattgca agatatgcag
     2821 tagaatcaga acaggaaata tgggtaattc ttaaaaagaa agcagacaga cctgatttta
     2881 tctacaatct ccagattgaa gatgaaatca acttatgcct accccatatt tcttcagaag
     2941 acgaaatcct taaaaaaagg aagtttctac caggcatggt tgaatacttt gttgatgtaa
     3001 ggggactggg tgaatcaatg ccagaagaaa agaaaaactt cttcagaccc tatggctatg
     3061 atttcatgat ggatatgtgt tacgcaatgt ttggagaaac ttattttggc aaaagaattt
     3121 ttgatgtgct ttcagtcatc aaattattgg aagaaaaagg atgtaaaacg attaacttat
     3181 atggaaatca tcaaggagca ctgattggat tgtttgttgc ttttctttct gtaccagtga
     3241 aaaaggtcta tcttaaaaat ctgccagagt ccttttgttc tctttgtaag aaattgtcca
     3301 ctgaacttcc atcggcaaat ttcccgaagg gaataatgaa aattacagat atccctgaaa
     3361 ttttaaatta tctgaagaaa accaaagaag cagttgttat aagataagcc cgatcttatt
     3421 ccgcaacaac actgacagaa gatttcaaat tgaccagata tcctgctcgg ttatacccgc
     3481 tttttcaatc attgatgctg ctttttccag atccagatta tatgattgat ggcagtcagt
     3541 gccaatggaa aatttcactc ctttggattt caaaaaagaa agatattcaa gatactgcat
     3601 tttaaagttt tccgggtatg atggatgaag aaaaattgcg tgcagattga tttcaacaat
     3661 tttgttattt ctaacgaccg catcagcgaa ttcatcatgc atactttttg gtatcacatt
     3721 aaaatcacta aaccatggtt caaattgtgc tacatatttt t
//
LOCUS       DAORYU010000142         3743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_16451_length_3743_cov_4.772505, whole genome shotgun sequence.
ACCESSION   DAORYU010000142 DAORYU010000000
VERSION     DAORYU010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3743
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16451_length_3743_cov_4.772505"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="PLW07_05195"
     CDS             complement(<1..622)
                     /locus_tag="PLW07_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013289435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOL49955.1"
                     /translation="MKGVILAGGLGSRLYPLTKVTNKHLLPVYDRPMIYYPLETLVKT
                     GIKDIMVITGRTSSGDFLRLLENGREFGLEHLTYAYQEGEKGIAHALALAEHFAAGEK
                     IVVILGDNIIEKDISKEVDIFSKQKEGARIILKEVDDPKRFGVAEIREDIIVKIIEKP
                     SVPPSKFAVIGIYMYDHQVFDFVKTLKPSARGELEITDVNNKYIEKG"
     gene            complement(622..1077)
                     /locus_tag="PLW07_05200"
     CDS             complement(622..1077)
                     /locus_tag="PLW07_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HOL49956.1"
                     /translation="MIQGVKVKKLSFNVDDRGRLLEILRNDDEIFESFGQVYITTCYP
                     EVIKAWHLHHLQNDNMVVISGMAKIVLCDQREGSKTFGEINEFIIGDFNPMLVHIPAN
                     VYHGFMCISEKEAVILNVPTQPYNHKSPDEYRLPFDTDKILYRWERKNR"
     gene            complement(1128..1691)
                     /locus_tag="PLW07_05205"
     CDS             complement(1128..1691)
                     /locus_tag="PLW07_05205"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOL49957.1"
                     /translation="MIEAANLKAGTFIKKEEEIFKVLEIEIKAGPARFSSHVHIKLLN
                     VKTGKIVEMKTPPEEKLEDLAIEIALLEYLYQDEDNFCFMNPQTYEQFEIPGHMFEHF
                     KEFLKEGTALKFEIYQGTPISVIIPETVELKVVSAGGGVKGETDATYKNAILENNMEI
                     LVPHFIKEGDVIKVSTSTKRYVERVHK"
     gene            complement(1725..3566)
                     /locus_tag="PLW07_05210"
     CDS             complement(1725..3566)
                     /locus_tag="PLW07_05210"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOL49958.1"
                     /translation="MFHGPGFHRTGRFRYDGDDEVKGTVRITRLFGYLKPYKYQISFA
                     LLISICVTVLNLVPPRIIGIIIDKALGKENLPILIKMCFSLLSIYIVVNLLNGVKIFL
                     MGKLGQKVTYDLWQEVYRNIQRLSFRFFDENQTGNIMARITSDITAVERVVVDGLDTT
                     IIASLTLIGITFVLFWMNWKLALITMIPVPLLVFMAYLITIKAHAIYREVRKKMGEIS
                     ALLQDSISGVRETRSFAREEYEINRLNTKSSEYVQTNIQAIKLWATFSPSIVITTTIG
                     TFLVLLFGGKMAIINQSITTGEIISFLFYLNLFYQPIHQLNMVNHMLQHARASSERIF
                     EIVDAEPDVKEAKGAMSFKRPVKGEVIFKNVHFSYKPGLEVLHGITFKANSGETIALV
                     GPTGAGKTTIVSLVPRFYDVDSGQICIDGVDIRKYKLKELRESIGIVMQEPFLFNGTI
                     LENIAYGRLDAKIDEIVEAAKLANAHTFIVKLADGYYHQIGERGVKLSVGEKQRIAIA
                     RAILKNPPILIFDEATSSVDNETEALIQEAISRLLKNRTSFVIAHRLSTVMNAHKILV
                     INEGNIVETGTHSELLVKGGLYKKLYEIQFKKTMTDPEYLNISNTNQ"
BASE COUNT         1019 a          763 c          681 g         1280 t
ORIGIN      
        1 ggcctttttc aatatattta ttgtttacat cagtgatttc aagttctcct cgtgcagaag
       61 gtttcaatgt ttttacaaaa tcaaagacct gatgatcata catataaata ccgatgacag
      121 caaattttga tggtgggaca gatggttttt caataatttt cacaattatg tcttcacgaa
      181 tttctgcaac gccgaacctt tttgggtcgt caacttcttt aaggataatt cgcgcgcctt
      241 ctttttgttt gctgaatatg tccacttctt ttgaaatatc tttttctata atgttgtcgc
      301 cgagtatgac gacgattttt tctcctgctg caaaatgttc tgcaagtgca agggcgtggg
      361 caattccctt ttctccttcc tgatatgcgt atgtaagatg ttcaagccca aactctcttc
      421 cattttcaag taacctgaga aaatctcctg aagaagtgcg tcctgtgatg accatgatat
      481 ctttgatgcc tgtttttaca agggtctcaa gcggatagta aatcattggt ctgtcgtata
      541 cgggcagaag atgcttgttc gttacttttg tcaggggata gagacggctt ccaagtccac
      601 cggcgagtat cacacctttc atcagcgatt tttcctttcc catctgtata aaattttatc
      661 ggtatcaaag gggagtcgat actcatctgg tgatttgtga ttatatggtt gtgtcggcac
      721 attaagtatt actgcttctt tttccgatat acacataaaa ccatgatata catttgcagg
      781 tatgtgcacc agcattggat tgaaatctcc gattataaat tcattaattt ccccgaacgt
      841 cttactgcct tcacgttggt cacagagaac aattttcgcc attcccgaga taacaaccat
      901 gttgtcgttt tgcaagtgat gcagatgcca cgcttttata acttctggat aacaggttgt
      961 tatatatacc tggccaaaac tttcgaaaat ttcgtcgtcg tttctcagaa tttcgagaag
     1021 tctacccctg tcgtctacat tgaatgatag tttttttacc ttaacaccct gaatcattta
     1081 tcccccctaa tatattatgg cacatttgta agtttgacaa atcatcctta tttatgaacc
     1141 cgttccacat accgttttgt tgaagtggat acttttatga catctccttc cttaatgaaa
     1201 tgaggaacaa ggatttccat attgttttca agtattgcgt ttttgtaggt cgcatcggtt
     1261 tcgcctttca caccacctcc tgcacttacc actttcaatt caacggtttc cggtataatg
     1321 acactgatcg gggttccctg gtaaatttcg aatttcagtg cagtgccttc ttttaaaaat
     1381 tctttaaagt gttcaaacat atgtccggga atttcaaatt gttcatatgt ttgaggattc
     1441 atgaaacaaa aattgtcttc gtcttgatat agatattcaa gcagggctat ttcaatggcg
     1501 aggtcttcca atttttcttc cgggggtgtt ttcatttcaa caattttgcc tgtttttaca
     1561 ttcaatagtt ttatgtgaac atgacttgag aatctcgcag ggcctgcctt aatttctatc
     1621 tcaagaactt taaaaatttc ctcttctttc tttataaatg ttcctgcttt gagatttgcg
     1681 gcttcaatca tgacctcctc cctgtttcaa ggaaatcatt tgtattactg atttgtgtta
     1741 gagatattca gatattccgg gtctgtcatt gttttcttaa actggatttc ataaagtttt
     1801 ttgtacaatc cccctttaac aagaagttca ctgtgggtac cggtttcaac aatgttgcct
     1861 tcattaataa ccagtatttt gtgtgcgttc atcactgtgg acaatctgtg cgcgatgaca
     1921 aacgatgttc tattttttaa caatctggaa atggcttcct gtatcagcgc ctcagtttcg
     1981 ttatcaacag aagaagtggc ttcgtcaaaa attaaaatag gaggattttt tagaatcgcg
     2041 cgggcaattg cgattctttg tttttctccc acagaaagct ttactcccct ttcgccaatt
     2101 tgatgataat agccatcagc aagtttgaca ataaaggtgt gggcattcgc aagttttgct
     2161 gcctcaacga tttcgtctat ttttgcatca agtcgtccat aagcaatatt ttccaggatg
     2221 gtgccattga ataaaaaggg ttcctgcatt acaataccga ttgattctct gagttctttt
     2281 aatttgtatt tacgaatatc caccccgtca atgcaaatct gtccagaatc aacatcataa
     2341 aaacgaggta ccagactgac aatggtggtt ttccctgcgc ctgttggtcc tacaagtgca
     2401 atagtttctc cagaattcgc cttaaaagtt attccatgaa gaacctccag gcctggtttg
     2461 tatgaaaaat gaacgttttt gaaaataact tcgcccttta cgggtcgttt gaaagacata
     2521 gcgccttttg cctctttgac gtctggttca gcatcaacga tttcaaaaat cctttcgctt
     2581 gatgcccttg catgttgaag catgtgattc accatattta actgatgaat tggttgatag
     2641 aaaagattga ggtaaaaaag aaacgagata atttctcctg tggtaatact ctggttaata
     2701 atagccattt tcccaccaaa aagaagtacc agaaaagtgc cgatggttgt cgtaattaca
     2761 atcgatggag aaaacgttgc ccataatttt attgcctgga tattagtttg gacatattcg
     2821 ctactttttg tgttcaatct gttgatttca tactcttctc ttgcaaaact tctcgtctcc
     2881 ctgacaccag aaatactatc ttgaagtaaa gcgcttattt cacccatttt ttttctaact
     2941 tctctgtata ttgcgtgtgc ctttattgta attaaataag ccataaaaac cagcaaaggt
     3001 actggtatca ttgttattaa cgcgagtttc cagttcatcc agaataatac aaaggtgatg
     3061 cctataagtg ttagagacgc aataatggtt gtatccaggc catctacgac aaccctttca
     3121 acagccgtaa tatcgcttgt aattcttgcc ataatgttac cggtttgatt ttcgtcaaag
     3181 aatctgaaag aaagccgctg gatatttctg taaacttcct gccagaggtc gtatgtgact
     3241 ttttgcccga gttttcccat caaaaaaatt tttactccat tcaataaatt aacgacaatg
     3301 taaatggaga gtaaagaaaa acacattttg ataaggattg gcagattttc tttgccgagt
     3361 gccttatcaa tgataattcc aatgattctt ggtggaacaa gattcaaaac agtgacgcat
     3421 atggaaatga gtaaagcaaa ggaaatttga tatttgtaag gtttgagata tccaaacaat
     3481 cttgtaatgc gaactgtgcc cttaacttcg tcatcaccat catacctgaa tcttcctgtt
     3541 ctgtgaaagc caggcccatg aaacatcaaa atctcccttg atttaattca aaacctattt
     3601 taatagcttc aggaaatttt tcaacttttt taccgggatg tgataggttt gaatttagta
     3661 cgtcaccgtt attacggatg aagtaggtcg cgcaggactc ggcgccctcg cctcccacta
     3721 cggctcgggt cgatccgccc tgc
//
LOCUS       DAORYU010000143         3734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_16495_length_3734_cov_4.742865, whole genome shotgun sequence.
ACCESSION   DAORYU010000143 DAORYU010000000
VERSION     DAORYU010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16495_length_3734_cov_4.742865"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /locus_tag="PLW07_05215"
     CDS             <1..1018
                     /locus_tag="PLW07_05215"
                     /inference="COORDINATES: protein motif:HMM:NF015177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HOL49959.1"
                     /translation="KNRTRSSGLYWFEDTGSSGMDNSPRSGYASQHLNGSDVCWIDLS
                     CQQALNAFYIAKIAQLLNKTRDEEFFLSEFQNLKNLINQYHWCEKHQFYYDVFSRNNP
                     SLRHNFLNVKTVASFWPIISFVANDNQINGLIQHLLNPDEFWTLHPVPSLAKSDPNYD
                     PFGSYWLGGVWAPTNYMIVSGLKKNMRNSIARKIALNHLIAMTEVFNDNTYSGIWEAY
                     SPEFFMPATVKETGNLVRNAFVGWSGLGPIAMLIENILGFTLDAHSNTIYWDISTACT
                     HGIKNLRFNKKSVSLVCHEKNGHQKRKIEIETSSALNVILSVEGTNICLKKTFGKGQH
                     FLWI"
     gene            1141..1213
                     /locus_tag="PLW07_05220"
     tRNA            1141..1213
                     /locus_tag="PLW07_05220"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1175..1177,aa:Glu,seq:ttc)
     gene            1242..1841
                     /locus_tag="PLW07_05225"
     CDS             1242..1841
                     /locus_tag="PLW07_05225"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOL49960.1"
                     /translation="MENIWIASWKHCDWVGKADLVILFLLSIYSWAIMIDKFVLLKRV
                     RRANERFISSFYNGKMVNVPGSPWSRILAKLLQEKARQNISEGNISNLIKRFADEEMS
                     HLNNKIDFLLSISAVAPFMGLLGTVWGILMSFHNIGQTGTSSVSIIASGISEALIVTF
                     VGLLVAIPAALGYNYLNGQVRNIQIQGNHVLEAFHIAIK"
     gene            1838..2263
                     /locus_tag="PLW07_05230"
     CDS             1838..2263
                     /locus_tag="PLW07_05230"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOL49961.1"
                     /translation="MNKNTFFDIEQSKENALNRINIINFIDVALVLVITLMIVSPMIE
                     QGIEVKLPVSGPGKMAVQRSVMLTVAKNGVVFWGNQQVSIENLEHIVNNAISQNPELG
                     IVIKGDKNITYQELISVLDILKRADVKKIGLATQAKTEK"
     gene            2260..3054
                     /locus_tag="PLW07_05235"
     CDS             2260..3054
                     /locus_tag="PLW07_05235"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOL49962.1"
                     /translation="MKKALLASILIHALFVILIVQNSTSKKQIIPVYVVDLMSLPPAI
                     EISNNVISTFKPEHAEASIVSTSHRNLPIQTGTHAVKTGGERIVGSTSSVEKFSPNDF
                     IAGINKKLSRGVSSPESEDTSSAGKSAPAQDSPAGKTSLASKIFPLTGTESETGSSIG
                     IQTSSLPAGNIIPLEYLENIKLMLQRKWKPPKGKNYSSTSVVSFKIKKNGTVEEIMIE
                     KSSGFKEFDESAIKAVKETTGIPPLPGTYKPEYLEIAVKFNVRGIE"
     gene            3051..>3734
                     /locus_tag="PLW07_05240"
     CDS             3051..>3734
                     /locus_tag="PLW07_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49963.1"
                     /translation="MNKWGVILLMCFAWTFSSLYGQNRITIEITSTTEMKTNAVILIG
                     ERQTNFLEEFCTQLAKDLNNSDWLETKNISFKENFNIQTELDDGLSKNNANILVLCKI
                     NKKLETLIYDTVEKSIVSQFTIAIGSSPIELAHVVSDEIVYRLTGKPGIARSKILYMT
                     KQNSEYCLMLAAYDGSNATKLLSTDYIINYPRWFPDMKNILFLSYRKTFPALEIFNLT
                     KNQIETFLAE"
BASE COUNT         1247 a          723 c          724 g         1040 t
ORIGIN      
        1 aaaaaataga acccgcagca gtggtcttta ctggtttgaa gatactggtt ccagcggtat
       61 ggataattct ccccgcagcg gttatgcatc acaacacctc aatggaagcg atgtttgctg
      121 gatagatctg tcctgtcagc aggcattgaa cgcgttttac atcgcgaaaa ttgcacagtt
      181 actcaataaa accagagatg aagaattttt tctttcagag tttcaaaatc tcaaaaatct
      241 tattaatcaa tatcactggt gcgaaaaaca tcagttttat tatgatgttt tctcaagaaa
      301 taatccttcc ctcagacaca attttctcaa tgtaaaaact gtggcttcat tctggccgat
      361 tataagcttt gttgcaaacg acaaccagat aaacggtttg atacagcatc ttttaaatcc
      421 agatgaattc tggacactgc accctgtacc atcgctcgca aagtctgacc caaactatga
      481 cccattcggt tcctactggc tgggaggcgt ctgggctccc acaaattata tgatcgtgtc
      541 cggattgaaa aaaaatatga gaaacagtat cgcaagaaaa attgccttga atcacctaat
      601 tgccatgaca gaggttttca acgataacac atactctggt atctgggagg catacagtcc
      661 ggaattcttc atgccggcaa cagtaaaaga aaccggaaac cttgtaagaa acgcgtttgt
      721 tgggtggagc ggacttggac ctattgcaat gctgatagaa aacattctgg ggttcactct
      781 cgatgcccat tccaatacca tctactggga tatctctact gcctgtaccc atggaatcaa
      841 aaatctgcga ttcaataaaa aatctgtttc tcttgtatgt cacgaaaaaa atggtcatca
      901 gaaaagaaaa attgaaattg aaacaagcag tgcattaaat gttatcctgt ctgttgaagg
      961 aacaaacatc tgtcttaaaa aaacctttgg aaagggacaa cattttttat ggatttgaac
     1021 ccgttattct gatgattaat gccctatttg taggaaacaa ataaacacct ttacccatgg
     1081 taatatacag atttcttgac tcaaaacagg tttatcctta taataaatag gcgtaaaaaa
     1141 gcccccatcg actagcggcc aaggtcacca ctctttcaag gtggtagcgc cggttcgatt
     1201 ccggctgggg gcgcttaaaa tcatatgggg atacagttat tgtggaaaat atatggatag
     1261 caagctggaa acactgtgac tgggttggta aagcagacct tgtgatactg ttcctgcttt
     1321 caatttatag ctgggcaatt atgattgaca aatttgtact gctgaaacgg gtcagacgcg
     1381 cgaatgaaag atttatttca agtttttaca atggaaaaat ggttaatgtg cctggctcgc
     1441 catggtcaag gatactggca aaacttttgc aggaaaaagc cagacagaat atttctgaag
     1501 gaaatatctc gaaccttatt aaaagatttg ctgatgaaga aatgtcccat ctgaacaata
     1561 aaattgactt tttgctatct atctctgctg ttgccccatt tatgggactt ctcggaacag
     1621 tctggggaat actcatgtct tttcataata ttgggcagac aggaacgtcc tcagtgtcga
     1681 ttatagcgtc aggtatttca gaagcattaa tagtgacatt cgtcggactc cttgttgcca
     1741 tccctgcggc acttggatat aattacttaa acgggcaggt gcgaaacata cagatacagg
     1801 gaaatcatgt acttgaagcg tttcacatag ccatcaaatg aacaaaaaca ctttttttga
     1861 tattgaacaa agtaaagaaa acgctctcaa cagaattaat ataatcaatt tcattgatgt
     1921 ggctcttgtc cttgtcataa ccctgatgat tgtatcgcca atgatagaac aggggataga
     1981 agtcaaactt ccagtttctg gtccaggtaa aatggctgtt caaagaagtg tcatgctgac
     2041 tgtggcaaaa aatggcgtgg tattctgggg aaatcagcaa gtatcaattg aaaaccttga
     2101 gcatatcgtc aataacgcaa tttcgcaaaa ccctgaactt ggcattgtca taaaaggaga
     2161 taaaaacatc acatatcagg aattgataag tgttcttgat attttgaaac gtgctgacgt
     2221 taaaaaaatt ggtcttgcca cacaggcaaa aactgaaaaa tgaaaaaggc tttgctggct
     2281 tcaatattaa tccatgcact ctttgttatt ctcattgtcc agaactccac gagtaaaaag
     2341 caaataatac ctgtatatgt ggttgattta atgagtttgc cacctgccat agaaatttcg
     2401 aataatgtaa tatctacctt caaacctgaa cacgcagaag catcaattgt tagtacatcc
     2461 catagaaact taccgattca aacaggcaca catgcagtaa agacaggtgg tgaaagaatt
     2521 gttggaagta catcctctgt tgaaaaattc tcacccaatg attttattgc gggaataaac
     2581 aaaaaactct cacggggcgt atcgtctccc gaatcagaag atacctcatc tgctggaaaa
     2641 tcagcacctg ctcaagattc cccagcaggg aaaacttctc tcgcgtcaaa gattttcccg
     2701 ctgacgggca ctgaatctga aacaggttca tccataggta ttcaaacatc aagtttgccc
     2761 gcgggtaata tcatacccct ggaatatctt gaaaacatta aactgatgct acagagaaag
     2821 tggaaaccac caaaagggaa gaattattct tcaacttctg ttgtgtcatt caaaatcaag
     2881 aaaaacggaa cggttgaaga aatcatgatt gagaagagtt cagggtttaa ggaatttgat
     2941 gaaagtgcta ttaaggcagt gaaagaaaca acaggcatac caccgcttcc aggaacttat
     3001 aaaccagagt atctcgaaat cgctgtgaag tttaacgtca gggggataga atgaataaat
     3061 ggggagtaat cttattaatg tgctttgcat ggactttttc ctctctttac gggcagaata
     3121 gaatcaccat tgaaatcaca tcaacaacag aaatgaaaac caatgccgta atactgatag
     3181 gagagaggca gacaaacttt ctcgaagaat tttgcactca acttgcaaaa gacctgaata
     3241 attctgattg gttagaaaca aaaaacatca gtttcaaaga aaattttaac attcagacag
     3301 aactggatga cggattatca aaaaacaatg cgaatatcct ggttctttgc aagatcaata
     3361 aaaaacttga aaccctgatt tatgatactg ttgaaaaatc tattgtttct caatttacaa
     3421 ttgccattgg cagttctccg attgaacttg cacacgttgt aagtgatgaa attgtctatc
     3481 ggttaacagg aaaaccagga attgccagaa gtaaaattct gtatatgact aaacagaata
     3541 gtgaatattg tttgatgctt gctgcctatg atggttcaaa tgcaacaaaa cttttgtcaa
     3601 ccgactacat tatcaattat ccaagatggt tccctgatat gaaaaacatc ctctttcttt
     3661 catacagaaa gacctttcct gctcttgaaa ttttcaatct gacaaaaaat cagattgaaa
     3721 cattcctcgc agaa
//
LOCUS       DAORYU010000144         3674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_16816_length_3674_cov_5.642166, whole genome shotgun sequence.
ACCESSION   DAORYU010000144 DAORYU010000000
VERSION     DAORYU010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16816_length_3674_cov_5.642166"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="PLW07_05245"
     CDS             <1..587
                     /locus_tag="PLW07_05245"
                     /inference="COORDINATES: protein motif:HMM:NF013355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49964.1"
                     /translation="TESLYSIGYRIFEIDFCPFLKEHDEMMNQKTILRWYREIPLMIK
                     KVFNDIQVFPKIMNLDYGLDFQIKMVESAISGSADGVVIANRIYKSDYSCAHGGIELR
                     ERNLAQIQTVHRLYPAFSISGTGGIYTGKHMIEYLDAGCENVQILSYLMGKVNKPFLK
                     KGNRFQQVFYQMMFDRDNGYLFYLIKRKKKHENR"
     gene            574..2574
                     /locus_tag="PLW07_05250"
     CDS             574..2574
                     /locus_tag="PLW07_05250"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOL49965.1"
                     /translation="MKIVENGIALCNIHIPETPYPVEIYAAKQLQTYLKKISGASLNI
                     VKGFPEHGPAICIAEKSHTEFDETKTMVPESVMKLVNKQRLFFVGGDRRGTLCCIYDF
                     LNRELGCVFAQPDIEYIPERTTIDVSEEFFYHQPFMKSRYIIGNGDEKMRQWCAKICL
                     SHSVPPQVNKTPEFQGERELFQEEFEKIWAYKAVEIQGESGISIGGHACESIMPPSKY
                     FQEHPEYFAYNPSQKPDALHKMKDGRDSFGICWTHPEVKKIFVEYFLNFFRKHPYIKR
                     FTFFPNDGQPPCFCEHCRKQEEPWQGITTRQLQYTKNYVLFAAEIAKAVAKEFPDVRI
                     EVGSYNGHTELPDDFDAELPPNLDVLFCIFERKWDRALDDPPTDEELRKTLAETTVST
                     YEKDAKKYTIYPEIFKKWRKHIKGMFRYYDYLTSTLGSMGMLFPVSKGTVRTIRFLKQ
                     QGFDGYGSQWFDSPVIWASYGLSLYVASRASWDESKTWEQLAKEYCLALYQEAATPMF
                     EYYETLENSARNVRFGMGIPEILQVFDDTTYKRCKEKLNNAMNLAKSGKIKKRIKEQK
                     ILLEFGHLFWQTREIEKKIESALKQDNIDACFTLVAQHAKIDDRIQKLFNAPLLSTYK
                     QWRGILYRHILGSTDDRRGLVHVIKLLKDATNARFHHLWYKD"
     gene            complement(2575..>3674)
                     /locus_tag="PLW07_05255"
     CDS             complement(2575..>3674)
                     /locus_tag="PLW07_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49966.1"
                     /translation="WEVPMMPGWPPQNPVEICCDIEHVRKCRMFAIDMLPEYDMKNRP
                     LFEKKVLDSWIHVSYGTLGWTEEVVPMLPIGFWDYGDNGANNEEMFGHVYFQNHLRTG
                     NFTCLENGIAIARHILEVDFVDFSIDSLQNGGMVAHCLNHNDGAVYPSHMWFTELLFA
                     YALTGDIEFKKAAIRICENLLQWIEDDRYFKIVSGDHRESGQPMINLTWCYHFNRDTR
                     YLKACEKIIKKNLMEQVKKYGKMFAPKPHSMPLKLVRYGDYATWEGMFWYWNITKDDE
                     VKNFMLSQLEWRLSNEYMTPFVYHRVSDINPAAYAYYMIGDRSYMDRVAKFIKASFRC
                     AKWPIGWIHSMYALKIAFDLGIISDDDIIPQ"
BASE COUNT         1190 a          701 c          725 g         1058 t
ORIGIN      
        1 cgactgaatc tctgtacagt attggctata gaatatttga aattgacttc tgcccttttt
       61 tgaaagaaca cgatgagatg atgaatcaga agacgatatt aaggtggtac agagaaatac
      121 cgctgatgat aaaaaaagtt ttcaatgaca ttcaggtgtt tccaaaaatt atgaacctgg
      181 attatggact tgattttcag ataaaaatgg ttgaatctgc aatcagtgga agcgcggacg
      241 gagtagttat tgcaaataga atttataaaa gcgactatag ttgcgctcat ggtggtattg
      301 aacttagaga gaggaatctt gctcaaatac agacggtaca tcgtctttat cctgccttca
      361 gcatttcagg aacaggcggt atttacacag ggaaacatat gattgaatac cttgatgcag
      421 gctgtgaaaa tgttcagatt ctcagttatc ttatgggaaa ggtgaataaa ccatttctca
      481 aaaaaggcaa tagattccag caggtcttct atcagatgat gtttgacaga gacaatggtt
      541 accttttcta tcttatcaaa cgaaagaaaa aacatgaaaa tcgttgaaaa tggtattgcg
      601 ctatgtaata tccatatccc ggaaacgcca taccctgttg aaatatatgc cgcaaaacaa
      661 cttcaaacat acctgaaaaa gatttctggc gcatcactca acattgttaa agggtttcct
      721 gaacatggac ctgcgatttg cattgctgaa aaaagccata ccgagtttga tgaaacaaaa
      781 acaatggttc ctgaatctgt aatgaaactg gttaataaac aacgattgtt tttcgttggt
      841 ggagacaggc gcggtacgct ctgttgcatc tatgattttc tcaacagaga acttggctgc
      901 gtttttgctc aaccagacat tgagtatatc ccggaaagaa ccacgattga tgtctcagaa
      961 gaattttttt atcaccagcc atttatgaaa agtaggtata ttattggtaa tggcgatgaa
     1021 aagatgcgtc aatggtgcgc aaaaatttgt ttgagtcatt ctgtgccacc ccaggtaaat
     1081 aaaactcctg aatttcaggg tgaaagagaa ctttttcagg aagaatttga aaaaatctgg
     1141 gcgtacaaag cagttgaaat acagggtgaa tcaggcatat ccataggcgg acatgcatgt
     1201 gaatccatta tgccaccgag caagtatttt caggagcatc ctgaatactt tgcatataac
     1261 ccttcacaaa aaccagatgc attacataag atgaaagatg gcagggattc ttttggtata
     1321 tgctggacac atcctgaagt aaaaaaaatt tttgttgagt attttttgaa ttttttcaga
     1381 aaacacccct atatcaaacg gttcacattt ttcccgaatg atggacaacc gccatgtttc
     1441 tgcgaacatt gcagaaaaca ggaagaacca tggcagggaa tcacaacacg ccaattgcag
     1501 tacacaaaaa actatgttct gtttgcagca gaaattgcaa aagcagtggc aaaagaattt
     1561 cctgatgtca gaattgaagt gggaagttac aatggtcata cagaacttcc tgatgacttt
     1621 gatgctgaac ttccgccaaa cctggatgta cttttctgta tctttgaaag aaaatgggat
     1681 agagcccttg atgacccgcc aacagacgaa gaattgagga aaacccttgc agaaacaact
     1741 gtttcaacat atgaaaaaga tgcaaaaaag tacacaatat atcctgaaat tttcaaaaaa
     1801 tggagaaaac acatcaaagg tatgttcaga tattacgatt atctgacaag tacattgggc
     1861 tcaatgggga tgctgtttcc tgtgagcaag ggtactgtga gaaccataag attcttgaag
     1921 caacagggat ttgatggtta tggctctcaa tggttcgatt cgcctgttat ctgggcatcg
     1981 tatggactgt ctctctatgt tgcaagccgt gcttcgtggg atgaaagcaa aacatgggaa
     2041 caacttgcaa aagaatactg tcttgcactt tatcaggaag cagcaacacc aatgtttgaa
     2101 tactatgaaa cacttgaaaa ttctgcaaga aacgtaaggt ttggaatggg aatcccagaa
     2161 atattgcagg tttttgatga tactacttac aaaagatgca aagaaaagct caacaacgcg
     2221 atgaaccttg caaaatcagg gaaaataaag aaaagaatca aagagcaaaa gattcttctt
     2281 gaattcggac atctattctg gcaaacacgg gaaatagaga aaaaaattga atctgccttg
     2341 aagcaggaca atattgatgc ctgttttact cttgttgctc agcatgcgaa aatcgatgat
     2401 agaattcaga aactgtttaa tgctccactg ctttcgacat ataaacaatg gcggggcatt
     2461 ttatacagac atatccttgg atcaactgat gacaggcgcg gacttgttca tgtaataaaa
     2521 ctgctgaagg atgccacaaa tgccagattt catcacctct ggtataagga ttaattattg
     2581 tggaataata tcatcatcgc tgataatgcc aaggtcaaat gcaattttca atgcatacat
     2641 tgaatgtatc cagccaatag gccattttgc acatctgaat gatgccttga taaattttgc
     2701 gaccctgtcc atatacgacc tgtcgcctat catatagtat gcataagcag caggattgat
     2761 gtcgctaaca cgatggtata caaatggtgt catatattca tttgacagcc gccattctaa
     2821 ctgacttagc ataaaatttt tcacctcgtc gtcttttgta atgttccagt accagaacat
     2881 tccttcccag gttgcataat caccatatct gacaagtttt aatggcattg aatgtggttt
     2941 tggagcaaac attttcccgt atttttttac ctgctccatc agattttttt tgataatttt
     3001 ttcacatgct ttaagatatc ttgtgtccct gttaaaatgg tagcaccacg taagattaat
     3061 catgggctga cctgattccc tgtggtctcc agaaacaatt ttaaagtatc tatcatcctc
     3121 aatccattga agaagatttt cacagattct tatggctgcc tttttaaatt caatatctcc
     3181 tgttaatgcg taggcaaaaa gaagttctgt aaaccacata tgagaagggt acacagcgcc
     3241 atcattgtga ttcaaacaat gagccaccat tccaccattt tgaagactgt caatactgaa
     3301 atccacaaaa tcaacctcaa gaatgtgtct tgcaattgcg atgccgtttt ccaggcaggt
     3361 aaaatttcct gttctcaggt ggttctggaa atagacatga ccaaacatct cttcattgtt
     3421 tgctccattg tctccataat cccagaaacc tatgggtaac attgggacta cttcttctgt
     3481 ccatccaagc gtgccataag acacgtgtat ccagctatcg aggacctttt tttcaaaaag
     3541 tggacgattt ttcatatcat attctggcag catatcaata gcaaacatcc tgcactttct
     3601 cacatgctct atgtcgcaac aaatttccac tggattttga ggaggccatc ctggcatcat
     3661 gggtacttcc catg
//
LOCUS       DAORYU010000145         3652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_16960_length_3652_cov_6.405616, whole genome shotgun sequence.
ACCESSION   DAORYU010000145 DAORYU010000000
VERSION     DAORYU010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16960_length_3652_cov_6.405616"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="PLW07_05260"
     CDS             <1..638
                     /locus_tag="PLW07_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49967.1"
                     /translation="TRDILIKNGFDPFRVISGPSLRYQYLFDFKNHWSDSSENRNILI
                     ICPLAIDAAVELLLKSIEAIKNLVNLYDNVYVKNHPMMNTSQLLEAAEIEKLPEKWEF
                     VSGGMEQWLKEVSCAVCMDSSAVLEVVVAELPVIIVGRETNLTMNPLSWLNDPEFQPV
                     FDAEQLQKRVVECLRITEEERKYLKVKSEEMLNRLSKANEDNLRVFTMAKS"
     gene            635..1510
                     /locus_tag="PLW07_05265"
     CDS             635..1510
                     /locus_tag="PLW07_05265"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOL49968.1"
                     /translation="MKRFVKGFYRWLTLFPHYVFAGYSKTRSLKEIKRIMDDYDMVKA
                     PSEDYFKQRYLAIINEVLRRNFTHNISLKILDAGCGQGRITIELAKMGHKIDAIDVVK
                     SALEKGKKYAEQQNVHNLINWQQGKLPECLSQYMDNGYDLVLCLEVLYMMSWSESKKT
                     LEELARKVKNNGVIMISVRPRFYYLAYSLMRNDFKKFRWCATHNDFLGLGEHLCWADV
                     NQMTKLLEQLGFRDLGMKGIRIFSGIEGDPTSFLAIPHLLSTEERRILSDVEDSYSNF
                     YTDHCRYTVFWGTRL"
     gene            1520..2623
                     /locus_tag="PLW07_05270"
     CDS             1520..2623
                     /locus_tag="PLW07_05270"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOL49969.1"
                     /translation="MIPVARPCLGEEELEEVRKVFESGWLGMGAWVKKFEDELKDFFN
                     VKHVIAVNTGTSAIHLALVSCGIKQGDEVIVPSLTFAATIQAIVATGASVVFCDVQPD
                     TLNLDPEDVKKRVSSKTKAIVPVHYRGYPCEMDQLKNIAKQHRLRIIEDAAHAFGSKY
                     RGKKIGSFGDVTCFSFDPIKIITCGEGGAIVTNDDDVAIAAEKGRILGINKDTWSRYK
                     HERSWFYDVETIGFRYHMSNINAAIGIVQMKKFPRFLERRLEIVKLYDENLKNINGIK
                     LLKTDYRSMAPFCYIIRAEQRDNLMAYLKKNDIDSGIHYIPNHLQSFFSKYKTSLPIT
                     EKIWNEILTLPLYYGLQNEQVLMICDRIKNFYS"
     gene            2626..3576
                     /locus_tag="PLW07_05275"
     CDS             2626..3576
                     /locus_tag="PLW07_05275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOL49970.1"
                     /translation="MAKVSVIVPVYNAERFIGETLRSVLCQTYKDIEVIVVDDGSTDH
                     SVEVIKKYNDPRIMLIRQQNRGVSAARNTGIKSSNGKFIALLDHDDLWLAEKIEKQMI
                     LFEKNPDVALVYSDISYIDGEGKPVSWMLKQFEPHRGYVLRELFVSDFIPCLTAVFRK
                     DVLEKIGLFNERYRYAEEYELFLRIARFFQFDFVDEVLACYRVHQSNLSRNIINSYLE
                     NIEILEHFYHTVPEVKNFEYTARKRIANMYFITGRRFQVEEKNRTEAIRMFRKSLIYN
                     KISIKAMVGLLTACLNVPVPRRHRILKCFTPKNIFDAHEK"
BASE COUNT         1240 a          535 c          785 g         1092 t
ORIGIN      
        1 ggacgcgtga tattttgatt aaaaatggtt ttgacccatt tcgtgttatt tcaggccctt
       61 cattaagata tcaatactta tttgatttta agaaccactg gtctgattcg tcagaaaaca
      121 gaaatatttt aatcatatgt ccacttgcaa ttgatgctgc tgtcgaatta cttttgaaaa
      181 gcattgaagc aataaagaat cttgtaaatc tttatgacaa tgtgtatgta aagaatcatc
      241 caatgatgaa tacttcacaa ttgttggaag ctgcagaaat agaaaagtta cctgaaaaat
      301 gggagtttgt ttcaggagga atggaacaat ggctaaaaga agtttcttgc gctgtgtgca
      361 tggatagttc agcagtgctt gaggtagttg tggcagagct gccggtgata attgtgggac
      421 gtgaaacaaa tcttacgatg aacccccttt catggctaaa tgacccagaa tttcaaccag
      481 tatttgatgc agaacaatta cagaaaagag ttgttgagtg tctgagaatt acagaggaag
      541 aaagaaaata tttgaaagtg aaaagtgaag aaatgttaaa cagattatca aaagcaaacg
      601 aagacaattt aagagttttc acaatggcaa aatcatgaaa agatttgtaa aaggatttta
      661 caggtggcta acgctatttc ctcactatgt ttttgctgga tatagcaaaa caagaagttt
      721 gaaggaaatt aaaagaataa tggatgatta cgatatggtt aaagcgcctt cagaggatta
      781 tttcaaacag agataccttg cgattattaa tgaagtcctc agacgaaatt ttactcacaa
      841 tatttcatta aaaatccttg atgctggttg tggtcagggt agaataacaa ttgaacttgc
      901 gaaaatgggt cataaaattg acgctattga tgttgtgaaa tcagcgctgg aaaaagggaa
      961 aaaatatgcg gaacaacaaa atgtacacaa ccttattaac tggcaacaag ggaaacttcc
     1021 agaatgtctt tctcaataca tggataatgg atacgatttg gttttatgtt tggaagttct
     1081 ttatatgatg tcctggagtg aaagcaaaaa aacactggaa gagcttgcca gaaaagtgaa
     1141 aaataacggg gttattatga tttcggtacg tcccagattt tattatcttg cctattcact
     1201 catgagaaac gatttcaaaa aatttcgatg gtgtgcgact cataatgatt ttttgggact
     1261 tggagaacat ttgtgctggg ctgatgtaaa tcaaatgaca aaacttttgg aacaattggg
     1321 atttagagat ctggggatga aaggaataag aattttttct gggatagagg gagacccaac
     1381 ctcatttctc gcaatacccc atcttctttc aacagaagaa aggagaattt tgtctgatgt
     1441 tgaggattct tacagtaatt tttatactga ccattgcaga tacactgttt tctggggaac
     1501 cagattatag gagaataaca tgataccggt tgcaagacca tgtcttggtg aagaagaact
     1561 tgaagaagtg agaaaggttt ttgaatcagg atggcttgga atgggtgctt gggtgaaaaa
     1621 atttgaggat gaattgaaag attttttcaa tgtaaagcat gtcattgcag tcaataccgg
     1681 aacatctgcc attcaccttg ctctggtctc gtgtggcata aagcagggtg atgaagtgat
     1741 tgtcccttct cttacttttg cagcaacaat tcaggcaatt gtggcaacag gtgcttctgt
     1801 agttttttgt gatgtccagc cagatacact gaaccttgac cctgaagatg tcaaaaaaag
     1861 agtaagctca aaaacaaagg caattgttcc tgtacattat cgcggttatc cctgtgaaat
     1921 ggaccaactg aaaaacatcg caaaacaaca tcggctaaga attattgaag atgcagcaca
     1981 cgcattcggt tcaaaataca gaggaaaaaa gattggttct tttggtgatg tcacatgttt
     2041 cagtttcgat cccattaaaa tcattacctg tggcgaggga ggtgcaattg ttacgaatga
     2101 tgacgatgtt gcaatagctg ctgaaaaggg aagaattctt ggaatcaata aagacacatg
     2161 gtcacgctat aaacacgaaa gaagctggtt ttacgatgtt gaaacaattg gcttcagata
     2221 tcatatgagc aacattaatg cagcaatcgg tattgttcaa atgaagaaat ttccgcgatt
     2281 tctggaaaga agacttgaaa ttgtaaaatt gtatgatgaa aatttgaaaa atatcaatgg
     2341 cataaaactt cttaaaactg attataggtc aatggcaccg ttttgctata ttatcagggc
     2401 agaacaaagg gacaacctaa tggcatatct gaaaaaaaat gatatagata gtggtattca
     2461 ctatattccc aatcatcttc aatcgttttt ttcaaaatat aaaacatcac tgcctattac
     2521 tgaaaaaatc tggaatgaaa ttcttacgct gcctctgtat tacgggttgc agaatgaaca
     2581 ggtattgatg atatgtgaca gaattaaaaa tttttattct tgaaaatggc gaaagtaagc
     2641 gtaatagtcc cggtttataa tgctgagcgt tttataggag aaacactgag aagtgttttg
     2701 tgtcagacat ataaagacat agaagtgatt gtggttgatg acggttcaac ggatcattct
     2761 gttgaggtga taaaaaaata caatgatcca agaataatgt taataagaca gcaaaacaga
     2821 ggagtttctg cggctcgtaa tacaggaatt aaatcttcaa atggcaaatt tattgcatta
     2881 cttgaccacg atgacctgtg gcttgctgaa aaaattgaga aacaaatgat cctgtttgaa
     2941 aaaaatcctg atgtggctct cgtgtattca gatatcagtt atattgatgg cgaaggtaaa
     3001 ccagtatcat ggatgctaaa acagttcgag ccacatcggg gttatgtgtt aagagaactt
     3061 tttgtcagcg attttatacc ctgtcttact gctgttttta gaaaggatgt tcttgaaaaa
     3121 atagggcttt ttaatgaaag atataggtat gctgaagaat atgagttgtt tttgagaata
     3181 gcaagatttt ttcaatttga ttttgttgat gaagttcttg cctgttacag ggttcatcaa
     3241 agcaatctca gtagaaatat cattaacagt taccttgaaa atattgaaat acttgaacat
     3301 ttttatcaca cggttcctga ggtgaaaaac tttgaatata ctgcgagaaa aagaattgcg
     3361 aatatgtatt ttattacagg aagaagattt caggttgaag aaaaaaacag gactgaagcg
     3421 ataaggatgt ttagaaaatc gttgatttat aataaaattt caatcaaagc catggtcggt
     3481 cttcttacag cgtgtctcaa tgttccggtt ccgagaaggc acaggatttt gaaatgtttt
     3541 actcctaaaa acatttttga tgctcatgag aaataactcc tgcggggaaa ggccctgcgt
     3601 aatagttttc gtgatatcca gccttggatg tggtggggca gaaaaggttg tt
//
LOCUS       DAORYU010000146         3633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_17076_length_3633_cov_3.231135, whole genome shotgun sequence.
ACCESSION   DAORYU010000146 DAORYU010000000
VERSION     DAORYU010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17076_length_3633_cov_3.231135"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1320
                     /locus_tag="PLW07_05280"
     CDS             <1..1320
                     /locus_tag="PLW07_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49971.1"
                     /translation="IHGKYIFADNIQIKCDASMPAGTSIELKYRTGTTRIYSPEIWSA
                     WKPLRKKTSNLWTINRSLKERFFQIQAILSTKNSARAPSIEKLQIAADVQYQPADESI
                     KAVLFNNPQIITTSIPFAYQEINYRTRALRSMYNLDQVIKDGKTEIEKFVMLRNWARH
                     TAPKGWDWGTSMWCPPWDALVILATNKQPAALCMCTHYSTLFTQCAIALGYTARQVIL
                     DHHCVAEIWSNELGKWILMDTGNSQNPEMNCHLEHNGIPLNAVEIRSLWRQNRTNEIK
                     FVYANETGITEKEKKDFETTYFKNFRRFAIPLRNNFLGNPFPGEPEQGQSEYYCDLYL
                     WWEDTPEPLESPEYGKTSNRISDFYWTLNQTFIDLVYSEKDTITVNLCNNTPQFSHYL
                     VSIDGGLWKKMPAQFLWKIHDGKNEMAVKSVNLFGLECQVSRAVIQK"
     gene            complement(1322..3250)
                     /gene="rlmD"
                     /locus_tag="PLW07_05285"
     CDS             complement(1322..3250)
                     /gene="rlmD"
                     /locus_tag="PLW07_05285"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOL49972.1"
                     /translation="MDIVLASQSERRKQLLKKLFPVFEVAIPDVDEIIDAALEPELVA
                     ILNAEKKAKNVGRKLNNKNCTIIAADTIVVIEDKILGKPAHYGTAFEYLHLLSDSFHR
                     VITGVCLFNPCENKILSDYDLTWVKFKKLDEQQIKSYLAKQTFYDKAGGYAVQEVNDE
                     FIEEIKGDFDNVVGLPVEKLKNMLAQFSGLREIEIHDIALPNRWGVGKQNEKIVFVDN
                     AVPGDNVWIQIVKDKRDFFYARNCGIKKHSEFRVNTKCPYFGTCGGCSFQNLDYNRQL
                     ELKKKYIVETLTRVGKIDYEIDVSPVIPSPDIYFYRNKMEFAFGQRNKKLVLGLRERQ
                     NPLKRYRAMVNSIDECAIFSELVKKVFPSVIEYVRQNNFEPYNPITKKGFIRHLVMRH
                     SKSTDQLMIILVTRPGMVNNLEKFVEELVNTVPNVTSFWHVENDQISDVVSFQKKRLI
                     FGKPYIEEHIENFKFIIYPETFFQPNTGGASRLYKTISLLAHEIKPESVLGLFCGSAP
                     IEIFLSRVCNEITGVDNNPANIETARENCRINNITNCKLYDMSAEEFLKQMQSSNSGY
                     NLLVVDPPRGGLSNKAIHQILKMRTKNIIYVSCNPATLARDLVLFGRAGYVLQKVIPV
                     DLFPHTSHIENCCVLRLT"
     gene            3315..>3633
                     /locus_tag="PLW07_05290"
     CDS             3315..>3633
                     /locus_tag="PLW07_05290"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOL49973.1"
                     /translation="MVLKYLNEDELFKVAMNIEKQGIDFYQNAFTKAIDLKTKEIFEF
                     LSNEEKRHYETFRTMDTEIQRIKFRPEAIDEEISLYLRSLVDSGIFENLMPRNQWKNI
                     TDRE"
BASE COUNT         1167 a          690 c          672 g         1104 t
ORIGIN      
        1 atccatggaa agtatatttt tgcagataac attcaaataa aatgtgacgc ttctatgccc
       61 gctggtacat caatagaatt gaaatataga acagggacga ccagaattta ttcccctgaa
      121 atctggtccg cgtggaagcc attacgaaaa aaaacaagca atttgtggac cataaaccgt
      181 tcattgaagg aaagattttt tcaaatacag gcaatattga gtaccaaaaa ttctgccaga
      241 gcgccgtcca ttgagaaatt acagattgct gctgatgtac aatatcagcc agccgatgaa
      301 agcatcaagg ctgtcctatt taataatcca cagatcatca cgaccagtat tccttttgcc
      361 taccaggaaa tcaattatag aacgcgtgca cttcgttcaa tgtacaatct tgaccaggta
      421 ataaaggacg gaaaaacaga gattgaaaag tttgtaatgt tgcgaaactg ggcaagacac
      481 acggctccaa aaggatggga ctggggaacc agtatgtggt gtccgccgtg ggatgcctta
      541 gtaatccttg ccaccaacaa acaaccggct gcgctatgta tgtgtaccca ttattcaaca
      601 ctgtttacgc agtgtgcaat tgcccttggt tatacggcac gacaggttat tcttgatcat
      661 cattgcgttg ctgagatctg gagtaatgaa ctgggtaaat ggatactgat ggatacaggt
      721 aattcgcaga accctgaaat gaactgccac cttgaacaca atggtattcc tctcaatgct
      781 gttgaaatca ggagcctgtg gagacaaaac agaaccaacg agattaaatt cgtgtacgca
      841 aatgagacag gaatcacaga gaaagaaaaa aaagattttg aaacaacgta ttttaaaaat
      901 ttcagaagat ttgcaatccc attaaggaac aattttcttg gtaatccctt tcccggtgaa
      961 ccagagcagg gtcagagtga atactattgt gatctgtacc tctggtggga agatacaccc
     1021 gaaccactgg agtcaccaga atatggaaaa acgagtaata gaatcagcga tttttactgg
     1081 actttgaatc agacctttat tgatttggtt tattctgaga aagataccat cactgtcaac
     1141 ctttgtaaca atactccaca attttctcat taccttgtca gtattgatgg tggtctctgg
     1201 aagaaaatgc cagcgcaatt cttatggaaa atccacgacg gtaaaaatga aatggcagta
     1261 aaatctgtca atctgtttgg tcttgagtgc caggtgtcca gagccgtgat tcaaaaataa
     1321 atcaggtgag ccgcaacaca caacagtttt caatatgcga agtgtgtgga aacaaatcca
     1381 cgggtataac cttttgtaaa acatatccag cccgaccaaa caaaacaagg tctcttgcaa
     1441 gcgttgcagg attgcacgaa acataaatga tattttttgt tctcattttt aaaatttgat
     1501 gaatggcttt attgcttaat ccaccgcgtg gagggtcaac gacaagaagg ttataccccg
     1561 aattactgga ttgcatttgt tttaaaaatt cttctgctga catgtcgtaa agtttacaat
     1621 ttgttatatt attaatcctg caattttctc ttgcggtttc gatatttgca ggattattat
     1681 caacgcctgt aatctcattg caaacacggg aaaggaaaat ttctataggc gcactaccac
     1741 agaagagacc cagcacagat tctggcttga tttcatgtgc cagaagggat attgttttat
     1801 acaatctgga agcgccgcct gtattcggct gaaaaaatgt ttcaggataa atgataaatt
     1861 tgaaattttc aatgtgttct tctatatatg gtttaccaaa aatcagacgc tttttttgaa
     1921 aacttacaac atccgaaatc tggtcatttt caacgtgcca gaacgacgta acattcggta
     1981 cagtatttac caattcttct acaaattttt caagattgtt caccatacca ggtctggtaa
     2041 caagaataat cattagttga tctgttgact ttgaatgtcg cattacaaga tgtcggatga
     2101 accctttttt tgtgatcggg ttatatggtt caaagttgtt ttggcgcaca tattcaataa
     2161 cagaaggaaa aacctttttt accagttccg aaaagatagc gcattcatca atgctgttca
     2221 ccattgctct atatcttttt agtggatttt gcctttctct caatccaagt acaagttttt
     2281 tgtttcgctg accaaaagca aactccattt tatttctgta aaaataaata tcaggcgatg
     2341 gaataacagg cgaaacatca atttcataat ctattttccc aacccttgta agggtttcaa
     2401 cgatatattt ttttttcagt tcaagttgac gattgtaatc aaggttctgg aaagaacatc
     2461 cgccgcatgt tccaaaataa ggacactttg tgttgaccct gaattccgaa tgttttttga
     2521 ttccacaatt tctggcatag aaaaaatctc gtttatcttt tactatttga atccagacgt
     2581 tatcaccagg cacggcattg tctacaaaca caatcttttc attttgcttt cctacgcccc
     2641 atctattggg caatgcgata tcgtgtattt ctatttctct cagaccgctg aactgagcca
     2701 gcatattttt caatttttcc acgggaagcc caacaacatt gtcaaagtcg cccttgattt
     2761 cttcaatgaa ttcatcattg acttcctgta ctgcatatcc accggcttta tcatagaatg
     2821 tttgctttgc aagatatgat tttatctgct gttcatccaa ttttttaaat tttacccatg
     2881 taagatcata atctgacaaa attttattct cacaggggtt aaaaagacag acaccggtta
     2941 ttactctgtg aaatgaatca gaaagaagat gtagatattc aaaagcggtg ccataatggg
     3001 ccggtttacc aagaattttg tcctcgataa caacgattgt gtctgctgca ataattgtgc
     3061 aatttttatt atttaatttt ctgccaacgt tttttgcttt tttttctgca ttcaaaatcg
     3121 caaccaattc cggttcaaga gcagcatcaa taatttcatc aacatcagga atcgcaacct
     3181 caaaaaccgg aaaaagtttt tttagcaatt gttttcttct ttcagattgt gacgcaagta
     3241 cgatatccat tttccactct gtttgactgt taaatttaaa atattagaat atattataaa
     3301 ccaaaggagg cgaaatggtg ttgaaatact tgaatgaaga tgaacttttt aaggtggcaa
     3361 tgaatattga aaagcaggga attgatttct atcagaatgc gttcaccaaa gcaatcgacc
     3421 tgaaaacaaa agaaatattt gaatttcttt ccaatgaaga aaaacgtcat tatgaaactt
     3481 tcagaacaat ggacactgaa atacaaagaa taaaattcag accagaagcg atagacgaag
     3541 aaatttcgct ttatttaaga agccttgttg attcaggaat atttgaaaac cttatgccaa
     3601 ggaatcagtg gaaaaatatt actgataggg aag
//
LOCUS       DAORYU010000147         3628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_17112_length_3628_cov_3.750350, whole genome shotgun sequence.
ACCESSION   DAORYU010000147 DAORYU010000000
VERSION     DAORYU010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17112_length_3628_cov_3.750350"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1610)
                     /locus_tag="PLW07_05295"
     CDS             complement(<1..1610)
                     /locus_tag="PLW07_05295"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF004556.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOL49974.1"
                     /translation="MNTKRFPYSQLSESEIKKLQEFSNLCRRDVLTMTTISKSGHPGG
                     SMSAMEIYNIVFHYAAISPEAPDDPDRDKILVSFGHTSPGVYAVLGRLGFFDLDTAIA
                     SFRQTGSIFEGHVVRKVPGVEWGTGNLGQGLSAGCGYALVDKMKNRDAFTFVLMSDGE
                     QTKGQVGEARRFAMKYKLDNIRVIIDYNRIQISGCIFDIMPNNIKENYLADGWEVMET
                     DGHDLGQLYSTIKNSLEVKKPVCIIASTTIGKGVSFMEGKCAYHGKTLGEEDYYKAVK
                     ELGLEGDLEKYKEIRKRKNYSFPKRNFDIKVSINQGKPRIYEPGTKIDNRSAFGNALL
                     DLGMVNKDNKENPIIVFDCDLASSVKTDLFAKQFPEKFFQCGVQEHHAATVSGAASVE
                     GAISFFADFGVFGVDETYNQHRLNDQNYTNLKIVCTHLGLDVGEDGKTHQCIDYIGLF
                     RNVFGMKLVIPADANQTDRVIRYVAAHPGNFFVGMGRSNTPVITTPDGKPLFGENYNF
                     EYGKADILRYGKQGYIYTMGALVPRAIA"
     gene            complement(1621..2991)
                     /locus_tag="PLW07_05300"
     CDS             complement(1621..2991)
                     /locus_tag="PLW07_05300"
                     /inference="COORDINATES: protein motif:HMM:NF027582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HOL49975.1"
                     /translation="MNRLFKEKYPDFRFLESSLRKHGDDYYFIAEKNGEMYLVIGNKS
                     FQFIEREKSIEILRNTFSFLAPACAGMKNSFGFGDRTGFATPGHIRAVKGSEFFPIFA
                     QQSARELERTGRTFKQVLDDAIFWCFVCGWSDGFGADADHAKSLDVLQQAISAGYTFF
                     TIDPSDKIKSHSEVSSCEKYNIERYCKEYAGKNFACEGFSFVFTEEMISDLATTYGSA
                     IDFIEECYTFISDQKNDFDFEVSVDETKVPTTPYAHVFIVMELKKRKIEFDSLALRFP
                     GRFEKGIDYIGDIGEFVRDLEIHNNIRKHFGLYKISLHSGSDKFSIYPVFKETLGNGF
                     HVKTSGTSWIQSLKTIAVVDREFFARCMEVALKDFEKNSKSYEISADISKVNSERMRN
                     ENLEEMFLDNNVRQLIHISYGSIIGNTPEGQKMKEHYYTLIKKNYELYFRFVEGHLKK
                     HVQLLS"
     gene            complement(2988..>3628)
                     /locus_tag="PLW07_05305"
     CDS             complement(2988..>3628)
                     /locus_tag="PLW07_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49976.1"
                     /translation="IKKPITQICYVVKEKKVYGIFAGDNEAWSAAADLSAQLNIEYVE
                     KSYKQVLSMAPVMYDDIWTAGKCMYKLEPIVSDGGSLIIYAPHITEVSYTHGKYIKQV
                     GYHTRDYFLKQWEKFKNFPGGILAHSTHVKGIGTFVDGVETPRINVHLATGIDQHVCQ
                     QINLGYISPDEINIDDFRKKQDTLVVENAGEILFRLKNGCVPDIDKLYERRK"
BASE COUNT         1072 a          829 c          544 g         1183 t
ORIGIN      
        1 atagcaattg cccttggcac cagtgcaccc atagtataaa tatatccctg tttcccgtat
       61 cttaaaatat ctgccttacc atattcaaaa ttgtagtttt ctccaaacaa tggttttcca
      121 tcaggagttg ttataaccgg ggtgttggaa cgtcccattc caacaaagaa atttcctgga
      181 tgtgctgcca catatcttat gaccctgtcg gtttgattgg catctgcagg tataacaagt
      241 ttcatcccga aaacatttct aaaaaggcca atgtaatcaa tacactgatg agttttccca
      301 tcctcaccta catccagacc aaggtgcgta catacaattt tcaaatttgt gtaattctga
      361 tcgttcaacc tgtgctgatt gtaggtttca tccaccccaa aaacaccaaa atctgcgaaa
      421 aaactgatag caccctcaac agaagcagcg cctgatactg ttgcggcatg atgctcctga
      481 accccacact gaaagaattt ttcaggaaat tgtttcgcaa acaaatctgt tttgacagaa
      541 ctcgcgaggt cgcagtcaaa gacaataata ggattttctt tgttatcttt gtttaccatg
      601 cccagatcca gaagtgcatt tccaaatgca cttctgttat caatttttgt acctggctca
      661 tatatgcggg gttttccctg attaatcgaa acttttatat caaaattcct tttcggaaat
      721 gaataatttt tcctcttccg tatctcttta tatttttcca ggtcaccttc taaccccagt
      781 tctttgactg ccttataata atcttcttct ccaagggttt ttccatgata ggcgcactta
      841 ccttccataa aagaaacgcc ttttccaatg gttgtacttg caataatgca aactggtttc
      901 ttgacttcaa gactgttttt tatcgtgctg taaagttgac cgagatcgtg cccatcagtt
      961 tccattactt cccatccatc agcaagataa ttttccttaa tgttattggg catgatatca
     1021 aaaatacaac cactgatttg aattctgtta tagtcaataa tgaccctgat attgtcaagt
     1081 ttatatttca ttgcaaaacg tcttgcttct cccacctgcc cttttgtctg ttcaccatca
     1141 ctcatcaata caaaagtaaa agcatcccta tttttcatct tatcaacaag tgcataacca
     1201 cacccagcag acaatccctg cccaagattt cctgttcccc actcaacccc tggtactttt
     1261 cttacaacgt gtccttcaaa aatactgccg gtctgcctga atgaagcaat tgccgtgtca
     1321 aggtcaaaaa aaccaagtct tcctaaaaca gcatacaccc cgggagaagt atgcccgaaa
     1381 ctgacaagga ttttatctcg atcaggatca tcaggcgctt caggacttat tgccgcataa
     1441 tggaaaacaa tattataaat ttccatcgca gacattgaac caccgggatg ccctgatttt
     1501 gaaatagtcg tcatcgtaag cacatctctt ctacacagat tgctaaattc ctgaagtttc
     1561 tttatttcac tttcagacaa ctggctataa ggaaatcttt ttgtgttcat ttttctcctt
     1621 tcatgaaagc agttgaacat gttttttcag gtgcccttca acaaaacgga aataaagttc
     1681 ataatttttt ttaatcagag tgtaataatg ttccttcatt ttctgccctt caggagtatt
     1741 gccaataata cttccgtaac tgatatgaat caattgcctg acattattat ccaaaaacat
     1801 ttcttcaaga ttttcatttc tcattctttc tgaattaacc tttgaaatat ctgcagaaat
     1861 ttcatacgat tttgagttct tttcaaagtc ctttagtgca acttccatac accgggcaaa
     1921 aaattccctg tcaacgactg caattgtttt caatgactgg atccatgaag tcccggatgt
     1981 cttcacatga aacccattgc caagtgtttc cttaaatacg ggataaatac tgaacttgtc
     2041 gcttcccgag tgaagcgaaa ttttgtacaa accaaaatgt tttcttatgt tattatgaat
     2101 ctcaagatct cgaacaaatt cacctatatc cccaatataa tctattcctt tttcaaatct
     2161 tcccggaaat cgtaatgcaa gactatcaaa ttcgatcttt ctttttttaa gttccatcac
     2221 aataaacacg tgtgcgtatg gagtcgttgg tacctttgtt tcatcaactg aaacctcaaa
     2281 atcaaaatca tttttttggt cagaaataaa tgtgtaacac tcttcaatga aatcgatagc
     2341 gcttccgtaa gtagtagcaa gatctgaaat catttcttca gtgaaaacaa aagaaaatcc
     2401 ctcacaggca aaatttttgc cagcatattc tttgcaatac ctctcaatat tatacttttc
     2461 gcatgatgat acctctgaat ggctttttat tttatcagat gggtctatag tgaaaaatgt
     2521 atatccagcg gaaattgcct gctgaagcac atctaaactt ttagcatggt cagcatctgc
     2581 gccaaatcca tctgaccaac cacagacaaa acaccagaaa atcgcgtcgt caagaacctg
     2641 cttgaatgtt cttcctgttc tttcaagttc tcttgctgac tgctgtgcaa aaatcggaaa
     2701 aaattcacta cccttcactg cccggatatg gccaggggtt gcaaaacctg ttctgtcacc
     2761 aaaaccaaaa gaatttttca ttcctgcaca ggccggtgcg agaaaagaaa aagtgttcct
     2821 caatatctca attgacttct cgcgttcaat aaactgaaaa gatttattac caatcaccag
     2881 atacatttct ccattttttt cagcaatgaa gtaatagtca tccccatgtt ttcttaatga
     2941 actttcaaga aatctaaaat cagggtattt ctccttaaat aaacgattca ttttctcctt
     3001 tcatagagtt tatctatatc aggtacgcat ccgttcttca acctgaaaag tatctctcct
     3061 gcattttcaa caacaagagt atcctgcttc ttgcgaaaat catcgatatt tatttcgtct
     3121 ggacttatgt accccaggtt aatctgctga caaacatgtt gatctatgcc tgttgcaaga
     3181 tgaacattga ttcttggggt ttcaactcca tcaacaaacg ttccaattcc tttcacatgt
     3241 gttgaatgag caagaattcc acccgggaaa tttttaaatt tttcccattg tttgagaaaa
     3301 tagtcacgcg tatgatatcc aacctgcttt atatatttcc catgagtata agacacctct
     3361 gttatatgtg gtgcgtaaat aatcaaactt cctccatcag aaacaatcgg ttcaagtttg
     3421 tacatacatt taccagcagt ccatatatca tcatacatta ctggcgccat cgaaagcacc
     3481 tgtttataac ttttctcaac atattcaatg ttcaattgtg ccgataaatc tgctgccgca
     3541 gaccatgctt cattgtcgcc tgcaaagatt ccatagacct ttttttcctt tacaacatag
     3601 catatctgag ttatcggttt ttttataa
//
LOCUS       DAORYU010000148         3605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_17251_length_3605_cov_3.557465, whole genome shotgun sequence.
ACCESSION   DAORYU010000148 DAORYU010000000
VERSION     DAORYU010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17251_length_3605_cov_3.557465"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..1062)
                     /gene="era"
                     /locus_tag="PLW07_05310"
     CDS             complement(172..1062)
                     /gene="era"
                     /locus_tag="PLW07_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOL49977.1"
                     /translation="MKSGTVAVIGKPNVGKSTLVNALVGHKVSIVSRHPATTRFRILG
                     VKTTDVHQIVFIDTPGYERPRHKLGELMKDNVIAAIEEADLILAVIDVTRYDEEDREI
                     LERIKKSEKNVIVVINKIDRASPREKILTLIEHLNREFDFHEIIPCSALNVENVDDVE
                     KTIVKHLPEGEKLFPPEFTDSLPQTYKISEIIREKIFEQVYQEIPHSIAVEIEEMKPG
                     DKNPDMLVIMANIIVEKDNQKRIIIGKNGEKLKIVGSSARKEIELLLGKKVYLQLRVK
                     TVEKWRDRPDISGRFGYGSI"
     gene            complement(1059..2174)
                     /locus_tag="PLW07_05315"
     CDS             complement(1059..2174)
                     /locus_tag="PLW07_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013782479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOL49978.1"
                     /translation="MTPRERWQNIFHFRPVDRIFNMEFGWWTDTLIRWHNEGLPKEIN
                     TIEKGDKFFGFDKTLVVPVKLRLDPVFDTETIEKTEKYVIIRDSSGVIAKHFNDGTST
                     IPHYIKFPVQNRIDWIEFRKRLNPDSNRYPTEHQWQEFKQKAEHSEFPVIISGGSLFG
                     WIRNWMGFEHCLMAFYDQPDLMEEIVEYICEFVLKVDEKALNEVRIDAVSMWEDMAFK
                     TQPMISPALFKKYLVPRYRSLSERFKKAGIDLIFIDCDGNINELVELWLDAGINIMFP
                     LEINSGSDPVQLRKKFGKRVLLMGGVDKKALIAGPSAIARELEKIKPVVEEGGYIPHV
                     DHRVPPDVSLKNYLYYLEKKREIFKIYDWIIPLVDTQ"
     gene            2240..2548
                     /locus_tag="PLW07_05320"
     CDS             2240..2548
                     /locus_tag="PLW07_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49979.1"
                     /translation="MKSTQTSNLYKILFSFRSLSFQKSPLIPLLQRVKTEEGTRGAGG
                     DFLKFPCNFRSPSFTKGRLGWISEIPLCPPFTKGENVDPLFIKEGLGEISKSPSIPLL
                     "
     gene            complement(2612..3313)
                     /locus_tag="PLW07_05325"
     CDS             complement(2612..3313)
                     /locus_tag="PLW07_05325"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOL49980.1"
                     /translation="MKVLAISAHPDDIELFCAGTLAKMKKQGHSIAMAILCDGNKGHF
                     EIGPEQLAEIRKKEAENSAKILGAELFIGLFPDLELHVNQQAVEKVMDVIRQVDPDVI
                     ITHSPDDYMMDHVNAGKLAIDASFCATLPNYRTKVNKITKLIPIFFMDTASGMNFQPT
                     EYVDITDEMPVKEKMLLCHQSQYKWLKDHDNIDYVEFMKALNQYRGTQCGVKYAEGFR
                     QYLVWGRVKPYRILP"
BASE COUNT         1059 a          774 c          603 g         1169 t
ORIGIN      
        1 aaagggaaaa tatgaaaaaa ttttgcaaag ggggaaatga agaagaaatt ttgaaaagat
       61 ggagacctgg aggagggatt tgaaaagagg aaaaacaggt gaaggaattt tatcatgggg
      121 aaaataaaaa aagagggaga ataataaatg aatcttatta gaaagaattt ctcaaattga
      181 gccgtaacca aatctgccag aaatatcagg tctgtcccgc cacttttcaa ctgttttcac
      241 ccttaattgt aagtaaacct ttttcccgag gagaagttct atttcctttc ttgcgctgga
      301 accaacaatt ttcagttttt ctccattttt accgatgatt attcttttct gattgtcctt
      361 ttcaacaatg atatttgcca tgataacaag catatcagga tttttatctc ctggtttcat
      421 ttcttcaatt tctactgcga tagaatgtgg aatttcctga taaacctgtt cgaaaatttt
      481 ttcgcggatg atttcgctga ttttatatgt ctgtggaaga gaatcagtga attctggtgg
      541 aaaaagtttt tcgccttctg gcagatgttt cacaatggtt ttctcaacat catcaacatt
      601 ttcgacattc aaggcagaac atggaataat ttcatggaaa tcaaattccc tgttcaggtg
      661 ttcaatcagc gtgagaattt tttccctggg acttgcccta tcaattttgt taatcaccac
      721 aataacattt ttttctgatt tttttatcct ttcaagaatt tccctgtctt cttcatcgta
      781 tcttgttaca tcaatcactg ccaaaattag gtcggcttct tcaattgcgg caataacatt
      841 atccttcatc agttcgccaa gtttatgacg gggtctttcg tatccagggg tatcaataaa
      901 aacaatctga tggacatctg tcgtttttac tccaagaata cgaaatcggg ttgttgcagg
      961 gtgtcttgaa acaattgaaa ccttatgacc cacaagcgca tttacaagcg tggattttcc
     1021 aacatttggt ttgccaataa ccgccactgt accagatttc attgtgtgtc cacaagagga
     1081 atgatccaat cataaatttt gaaaatttcc ctctttttct caaggtagta aagataattt
     1141 ttcaaactga catcaggagg aaccctgtga tcaacatggg gaatataacc gccttcttct
     1201 acaactggct taattttttc aagttccctg gcaatcgccg atggtcctgc aattaatgcc
     1261 tttttgtcaa ccccgcccat tagaagaact cgcttgccaa atttttttct caattgcaca
     1321 gggtctgaac cgctattgat ttcaagtgga aacataatat ttatccctgc gtccagccac
     1381 aattcaacca gttcattaat atttccatcg caatctataa aaatcaaatc tatccctgct
     1441 tttttaaatc gttctgataa gcttctgtat cgtggaacaa gatatttttt gaataatgca
     1501 ggagatatca tcggctgtgt cttaaaagcc atgtcctccc acatgcttac agcatcaatt
     1561 cttacttcat ttaacgcttt ctcgtccacc tttaagacaa attcacagat atattcaaca
     1621 atctcttcca tcaggtctgg ctggtcgtaa aatgccataa gacaatgctc aaatcccatc
     1681 cagtttctta tccagccgaa caaactacca ccgcttataa tcacaggaaa ttctgaatgt
     1741 tctgctttct gtttgaattc ctgccactgg tgctctgtcg ggtatctgtt tgaatctgga
     1801 tttaaccttt ttctaaattc aatccagtca attctattct gtaccggaaa ctttatgtaa
     1861 tgaggaattg tgcttgtgcc atcattgaaa tgttttgcaa tcacaccact tgaatctctg
     1921 ataatcacat atttttcggt tttttcgatg gtttctgtat caaaaacagg atcaagcctc
     1981 aattttacag gaactacaag cgttttatca aaaccaaaaa atttatctcc cttctcaata
     2041 gtatttatct ctttcggcag tccttcattg tgccatctta ttaatgtatc ggtccaccag
     2101 ccaaattcca tattaaaaat tctatcaaca ggcctgaaat gaaaaatatt ctgccatcgt
     2161 tcccgcggtg tcatttacac tccctgtaac tgaaacatca caaataaaat ttaaagtccc
     2221 attcgtatta gaatggtata tgaaatccac ccaaacatca aatctttaca aaatcctctt
     2281 cagcttcaga tctctttcct ttcaaaaatc ccccttaatc ccccttttac aaagggtgaa
     2341 gactgaagaa ggaacaagag gggctggggg agatttctta aaatttccct gcaactttag
     2401 gtctccttcc tttacaaaag gaaggctggg atggatttct gaaatccccc tttgtccccc
     2461 ttttacaaag ggggaaaatg tagatcccct ctttataaaa gaggggctgg gggagatttc
     2521 gaaatccccc tcaatccccc ttttataaag ggggatacat aaaagaaaca aaaaacgaaa
     2581 taaaaatttt caaagaaatt taaaaaaata attacggcaa aatcctgtat ggtttaactc
     2641 ttccccagac aagatactgg cgaaatcctt cagcatattt cacaccacac tgagtacccc
     2701 tgtactggtt taaagccttc ataaattcca cataatcaat gttatcatgg tctttcagcc
     2761 acttgtattg actctgatga cagagaagca ttttttcttt tactggcatt tcatctgtaa
     2821 tatcaacata ttctgttggc tgaaagttca tgccagaagc agtatccata aaaaatatcg
     2881 gtataagttt tgtgattttg ttaacttttg tgcggtaatt tggcagggtt gcacaaaaag
     2941 aggcatcaat ggcgagcttt ccagcattca cgtggtccat catgtaatca tcaggcgaat
     3001 gcgtgataat cacatcaggg tcaacctgcc tgatcacatc cattaccttt tcaactgcct
     3061 gctgattcac atgaagttca aggtcaggaa aaagtccaat aaataactcg gcaccaagta
     3121 tctttgcaga attttctgct tccttttttc gaatctctgc aagttgttca ggtccaattt
     3181 caaagtgtcc cttgttaccg tcgcagagaa ttgccatcgc tatggaatgt ccctgctttt
     3241 tcatttttgc aagcgttccc gcgcagaaaa gttcaatatc gtcaggatga gctgaaatag
     3301 caagtacttt catattcctc ccttgttggt cttaacagga tgaatgtaac attcaaattc
     3361 tctgtaagat tcagcatgtg ccttgacatc tgtaagtttt cattcaactc cctttccttc
     3421 ttataaattt ccctatcact ctcccctctt tacaaaattc ccctctaact tcaggtctcc
     3481 ttcctttgta aaatccccct taatccccct tttgtaaagg gggagaatag taggaggaaa
     3541 aagaggggtc gggggagatt tcttaaaatt tccctgcaac tttaggtctc cttcctttac
     3601 aaaag
//
LOCUS       DAORYU010000149         3580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_17388_length_3580_cov_4.407092, whole genome shotgun sequence.
ACCESSION   DAORYU010000149 DAORYU010000000
VERSION     DAORYU010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17388_length_3580_cov_4.407092"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="PLW07_05330"
     CDS             complement(<1..1064)
                     /locus_tag="PLW07_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49981.1"
                     /translation="MNKYRVIYNQDCTNLFHITQPIQPEHVKLMVDEVADGGVDLMLI
                     NPNAQKTNYPGDVWETYWEEGDFNDCVKQMRIFAEQGHDYLKLALQRCREKSIAPGVS
                     IRMNDMHGAQQPDTHPQNSRFYKSHPEYRLLGPVNNGWGRIGLNYEHEPVRLHYLALI
                     REIVERYDFDVLELDFMRFSDYFPFRDYEKHCAVMTEFIKQVRKITNQKQDIALYIRV
                     ASTPAAAYELGFDLETLVAKKFIDGIIFTEFLNTGWEMPVDEFRAIVGQNVALYAGAD
                     VSADRRPGLPIRYMPHNELLMRGFAYAYLSSGADGVYFFNFFTLRESGKDSGQLRFDI
                     FKQIKSIETMRNCPKAYLITS"
     gene            complement(1076..2362)
                     /locus_tag="PLW07_05335"
     CDS             complement(1076..2362)
                     /locus_tag="PLW07_05335"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOL49982.1"
                     /translation="MQITKLEKDVVVIGGGSAGSAAAIAVSRRGHRVLLVEETNCLGG
                     TATAGNVAQWYASMNGMGDIFNGIVERMKLFGGAIEHPYNPYNPEYLKIVWQLFAEEA
                     GVDILFHATLQDVICENGTVKSVRLVSCSKTIDVAGKFFIDASGEGDLGFLAGAEFMQ
                     GDPLKKRTLHMSLVFFLSDTGKSQTPYLPPGMKPINTDQELPGLHVHAKWFDGRIYCN
                     MTKIIDCDPTDPFSLSRAEMEARRQMIRIVHYLQRKYHPTYTICSSGARIGIREGRRI
                     IGDYILKKEDILGERPFDSDDGICVATSQIDLHSLTKPGHGGWRKKVLPYAIPFRCII
                     ARGFKNLLMAGKCISVDQVVHSSCRMIPTCCGMGQAAGTAVALGLESNVTDIRSLSVE
                     QLRNQLRLDGMELNPARHRAYCGEESDVPDEEKGLK"
     gene            complement(2374..3090)
                     /locus_tag="PLW07_05340"
     CDS             complement(2374..3090)
                     /locus_tag="PLW07_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49983.1"
                     /translation="MFTRDARRTVPLTMLLFLVPVLLSSGCLANSKEKDDVWGETKRP
                     PRENFQPVSSQISFDLSKSLLENPGLQPAESPEKLPKSWYGSLRVWKDVYKAPDIYSK
                     MVRIEQDEKISFQGKPSVKLCIAPSEEDVYLELYQEIRMKDKNLKGKKIRLSFYAYRE
                     KPPTGKELFLTGIMFNKEGQVVGTTVDIKPIIAPGKWIYFSEEGMVKPETELFKFSLR
                     FVSGGNDTVWFSGFLLEETQ"
     gene            complement(3111..>3580)
                     /locus_tag="PLW07_05345"
     CDS             complement(3111..>3580)
                     /locus_tag="PLW07_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49984.1"
                     /translation="IGLLPDISFQDGSASKKVRMIIPEGYQVFDMRNTKPVNLEQIVL
                     EPGIAQFYSLLPYRVENIEIKAQSEIKAGENMMMSFSINASKGVPENHVLRVEVYDPS
                     DREISWYSSNLVSTKGRAEHSINLPLNAEKGKWKIIAKDLPSGLTKTKEFEVK"
BASE COUNT          994 a          836 c          629 g         1121 t
ORIGIN      
        1 gaagtaataa gatatgcttt tggacaattc ctcattgttt caatgctttt tatttgtttg
       61 aatatatcaa atctgagttg cccggaatct ttgccggatt ctctcaatgt aaaaaaatta
      121 aaaaaataga caccatcagc gcctgaactt aaataggcat atgcgaatcc cctcatgaga
      181 agctcattgt ggggcatgta tcttatagga agcccaggtc ttctgtccgc agaaacatct
      241 gcgccagcat aaagagcaac gttttgacca acaatagccc tgaattcgtc cacaggcatt
      301 tcccatccag tgttcaaaaa ttcagtaaag attataccat caataaattt ttttgctacc
      361 aatgtttcaa ggtcaaaacc cagttcatat gcggctgcag gtgttgaagc aacccgaata
      421 taaagggcaa tatcctgttt ctgatttgtt atctttctta cctgtttgat gaattctgtc
      481 ataacggcac agtgcttttc ataatctctg aaaggaaaat agtcagaaaa acgcataaaa
      541 tccaactcaa gcacatcaaa gtcatatcgt tccactattt ctctgatgag tgccaggtag
      601 tgtaatctca ccggttcatg ctcataattt aatcctattc ttccccatcc attgttcaca
      661 ggacccaaaa gtcgatattc aggatgactt ttgtaaaatc gactgttttg cgggtgtgtg
      721 tcgggttgct gcgccccatg catgtcattc attctgatac tgactccagg agcaatactt
      781 ttttctctgc atcgctgtag ggcaagtttc aagtaatcgt gcccctgttc tgcaaaaatt
      841 ctcatctgct ttacacaatc attaaaatct ccttcttccc agtaagtttc ccagacatca
      901 ccgggataat ttgttttttg agcatttgga ttgataagca tcaaatcaac gccaccatca
      961 gcaacttcat ctaccattaa ttttacatgt tcgggttgaa tgggttgcgt aatatgaaaa
     1021 aggtttgtgc aatcctgatt gtaaattacc ctgtatttat tcattctcct gaacactatt
     1081 ttaagccctt ttcttcatca ggtacgtcac tttcttctcc acaatatgcc ctgtgccttg
     1141 ccgggttaag ttccatacca tcaagacgaa gttgatttct taattgttca actgaaagtg
     1201 acctgatgtc ggtaacattt gattcaagac caagggcaac cgctgttcca gccgcctgtc
     1261 ccataccgca acaggttgga atcatacggc aactactgtg cacaacctgg tcaacactga
     1321 tacatttacc tgccatcaaa aggtttttga aacccctggc gattatgcag cgaaaaggaa
     1381 ttgcataagg tagaaccttt tttctccagc caccatgccc gggcttcgtg agactgtgaa
     1441 ggtcaatctg ggatgttgca acacatatac catcatcact gtcaaagggt ctctcgccta
     1501 aaatatcctc tttcttcaaa atataatctc caataatccg tctgccttct ctgataccaa
     1561 tcctggcgcc tgaagaacaa atcgtatagg ttggatggta ttttctctgt agataatgaa
     1621 cgattctaat catctggcgt cttgcttcca tctctgctct ggaaagagaa aaaggatctg
     1681 ttggatcgca gtcaataatc ttggtcatat tgcagtaaat cctgccatca aaccactttg
     1741 catgaacatg caatccaggc aattcttggt ctgtgttgat tggtttcatt cctggaggaa
     1801 gatatggtgt ttgagatttc cctgtatctg acaggaagaa aaccagactc atatgaagtg
     1861 tgcgtttctt caacgggtct ccctgcataa attcggcacc agccagaaaa ccaaggtctc
     1921 cttcgcctga tgcatcaatg aaaaatttcc ctgcaacatc aatggtcttt gaacaggaaa
     1981 ccagtcgcac agatttaaca gtaccatttt cacatatcac atcctgtaac gttgcatgga
     2041 acaaaatatc aacgcctgct tcttcagcaa aaagttgcca tacaattttc agatattcag
     2101 gattgtatgg attatatgga tgctcaattg ccccgccaaa aagcttcatc ctttcaacaa
     2161 ttccattgaa tatgtcgccc attccattca ttgacgcgta ccattgcgcc acatttcctg
     2221 ctgttgccgt gccaccaaga caattggttt cctctacaag aagcaccctg tgacctcttc
     2281 ttgaaactgc aattgctgca gcagaacctg cagaaccgcc gccaataaca acaacatctt
     2341 tctcaagttt tgtgatttgc attggaaatc cttttattga gtttcttcta acaaaaatcc
     2401 tgaaaaccag acagtatcat ttccacctga aacaaatctc agagaaaatt tgaaaagttc
     2461 agtttctggt ttcaccatgc cttcctcaga aaaataaatc catttaccgg gagcaataat
     2521 cggtttgata tcaactgtgg taccaaccac ctgtccctct ttattaaaca tgatacctgt
     2581 caaaaaaagt tctttccctg ttggtggttt ctcgcgataa gcataaaaac tgagccggat
     2641 tttcttccct ttcagatttt tgtccttcat tctgatttcc tgataaagtt caagatacac
     2701 atcttcttct gatggagcaa tacagagctt cacagaaggt ttaccctgaa aagatatttt
     2761 ctcatcctgt tctattcgaa ccattttgct ataaatgtca ggcgccttat aaacatcctt
     2821 ccacacacgc aaactgccgt accacgactt tggcagtttc tcaggacttt ctgctggttg
     2881 taatccagga ttctccagta aacttttgct taaatcaaag gaaatctgtg aagatacagg
     2941 ttgaaagttt tcccttggag gacgctttgt ttctccccag acatcgtctt tttcttttga
     3001 gttggcaagg cacccactgg ataataacac tggtacgaga aataacagca tggttaatgg
     3061 cactgttctt cttgcatccc tggtgaacat ttttcactcc cagaagttta ttacttaact
     3121 tcaaattctt ttgttttcgt caaaccggat ggcaggtcct ttgcaataat tttccacttt
     3181 cccttttcgg cgttcaatgg cagattgatt gaatgttctg ctctgccctt tgtagaaaca
     3241 aggtttgatg aataccatga aatctctctg tctgacgggt cataaacctc cactcttaac
     3301 acatggtttt caggtactcc ttttgacgca ttgatagaaa acgacatcat catgttctca
     3361 cctgctttta tttctgactg cgccttgatt tcaatgtttt ccaccctgta cggaagcaaa
     3421 ctgtaaaatt gtgcgatacc cggctcaagc acgatttgtt ccagattaac gggctttgtg
     3481 tttctcatat caaatacttg atatccttca ggtattatca ttcttacttt tttgctcgca
     3541 gaaccatcct gaaaactgat gtcaggaagc aatccaatga
//
LOCUS       DAORYU010000150         3566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_17479_length_3566_cov_5.868414, whole genome shotgun sequence.
ACCESSION   DAORYU010000150 DAORYU010000000
VERSION     DAORYU010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17479_length_3566_cov_5.868414"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1141)
                     /locus_tag="PLW07_05350"
     CDS             complement(<1..1141)
                     /locus_tag="PLW07_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1926 domain-containing protein"
                     /protein_id="HOL49985.1"
                     /translation="MKPRFPGVLLCCLFSFFLQIQCAAKVLTQIDIDQDQEQEIVVEN
                     TFYRFVIKPSLGKIDSFVIKPSDIELLRDGEGKKERGALLADYIVQQGSFGDFMNQPY
                     IAEILENSDNKAVVRLSRRGNTDLLKWITITRTITAFADQPEIKVEYEIKNEQASMED
                     YYLGLGIHNEFDSSQELIFSMPLSTGVLHKKMTALTKSGTGEIFENIARGWVAISNEN
                     GGGIAFNFDYSVLKDFYEWHKMSLRSLAFSFLTKKIEPGKSFKTEFTILPYTSFIQVD
                     GVVKKYAGSIRIDAMPEKGKKVPVQVLLSGKSAPVDLRMYVMLPDGKEQPINNVSLTR
                     STDGLMVFKAEFTPQEEGLFVIKAIVLDGKEILGEFEKPIKLKDTG"
     gene            complement(1172..1849)
                     /locus_tag="PLW07_05355"
     CDS             complement(1172..1849)
                     /locus_tag="PLW07_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007276711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOL49986.1"
                     /translation="MKKGFTLIELLVVIAIIAILAAMLLPALSKAREKARQGVCMGNL
                     KQIGLAIFMYASDHNGYLVPFYSGTGVSPDFGIPWSKILLTYMSNANPNDEIGVTILK
                     CPTYKYGKPYLGTTETASYGVNYYNNMRFGYCPYMKKIDRLPKTLWLVADAKANYTLS
                     PTNYPSYMNTALDKDGVIDSGSAFLPVWATMGLCFRHNGFANFLFIDGGVRPLKASTV
                     FTDMSIW"
     gene            complement(1877..2563)
                     /locus_tag="PLW07_05360"
     CDS             complement(1877..2563)
                     /locus_tag="PLW07_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOL49987.1"
                     /translation="MKKGFTLIELLVVIAIIAILAAMLLPALSKAREKARQAVCMGNL
                     KQIGLAIFMYANDHDGILVPFNAGSTTNVHPTNYWYKILADEYGINLVVPVTGIIGLT
                     FLRCPSFKMYRKYFTAQEMASYGANYRDGARFSYAGTKLMKLDRLPKTCYLVVDCYGT
                     YTISPLQYTRDIDVDGDGLNDSWSVVYPSWPYFGICLRHNGFANFLIVDGSVKPYTGK
                     DWATNRDNIW"
     gene            complement(2581..3261)
                     /locus_tag="PLW07_05365"
     CDS             complement(2581..3261)
                     /locus_tag="PLW07_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007276749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOL49988.1"
                     /translation="MKKGFTLIELLVVIAIIAILAAMLLPALSKAREKARQAVCMGNL
                     KQIGLAIFMYASDNNGILVPFNTGTSTTPSSVYWYDIIASYIGKSGIIGQTFLKCPTY
                     KYGDKYYGAMVYYSYGVNYCNNAAPNTRFCYSGNYTLKLDRLPKNLWLVADATNRYTN
                     TPLQFPTIDTDIDGDGVKDSYSASLPTWAHYGLCFRHGGFANFLFADGSVKPLKSRDW
                     VTRTGIDW"
     gene            complement(3268..>3566)
                     /locus_tag="PLW07_05370"
     CDS             complement(3268..>3566)
                     /locus_tag="PLW07_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49989.1"
                     /translation="DHSILPYFPSTLNGEQYTDDIEVVTYDEIPGIIEEKEAIHEIIQ
                     PFDEMGKIAIEKMESIIKKQETISQIVIQPDFIIKEKLEQRINAGLIKETVQRR"
BASE COUNT         1005 a          833 c          614 g         1114 t
ORIGIN      
        1 tgcctgtgtc tttcaattta atgggctttt caaactcgcc aagtatttct tttccatcca
       61 gaacaatcgc tttgattaca aaaagacctt cttcctgtgg agtaaattct gctttgaata
      121 ccattaaacc atcggtactt ctggttaaag aaacattgtt gattggttgt tctttaccat
      181 ccggcaacat cacatacatt ctcaaatcaa ccggtgcact tttgccactc aaaagaacct
      241 gtactggaac ttttttccct ttttcaggca ttgcatctat tcttattgaa cccgcatatt
      301 ttttcaccac cccgtcaacc tgaataaaag aagtatacgg taatattgtg aattcagttt
      361 tgaaggattt ccctggttca atttttttcg tgaggaatga aaacgcaagt gaacgcagac
      421 tcattttgtg ccattcataa aaatctttga gaaccgaata atcaaaattg aaagcgattc
      481 caccgccatt ttcatttgaa attgccaccc atcctcttgc aatattctca aaaatttcgc
      541 cggttccact tttcgtaaga gcagtcattt tcttatgcaa aacgcctgtg gacagtggca
      601 tggaaaaaat aagttcctga ctgctgtcaa actcattatg tattccaaga ccgaggtaat
      661 aatcttccat ggatgcctgc tcatttttta tctcgtattc aacctttatt tctggctggt
      721 cagcaaaagc agttatggtc ctggtgatgg taatccactt caaaaggtct gtattaccgc
      781 gacgactgag acgcaccact gctttgttat ctgaattctc aagtatttct gcaatatagg
      841 gctgattcat aaagtcgcca aaacttccct gctgaacgat gtaatcagcc agtaatgctc
      901 ccctttcttt ttttccctca ccatccctca gcagttcaat atcagacggt ttaatgacaa
      961 aagaatctat tttgccgagg gatggtttga tgacaaacct gtaaaaggta ttttcaacaa
     1021 caatctcctg ttcctgatcc tggtcaatat caatctgagt aagaaccttt gccgcgcatt
     1081 gaatttgcaa aaagaatgaa aacagacaac aaagcaatac acccggaaat cgtggtttca
     1141 cctgctcctc ctctatttca ataattgttt cttaccagat gctcatgtca gtaaaaacag
     1201 tgcttgcctt caatggtctc acaccgccat caatgaaaag aaaatttgca aatccattgt
     1261 gtctgaaaca aagacccatt gttgcccaga caggaaggaa cgcagaccct gaatcaataa
     1321 caccatcttt atcaagggcc gtattcatat aacttggata gtttgtaggc gaaagggtgt
     1381 agtttgcttt tgcatctgcc acaagccaca gggtctttgg caacctatca atttttttca
     1441 tatacggaca gtagccaaat ctcatattgt tgtaataatt gactccataa gaagctgttt
     1501 cagtcgtgcc aaggtatggc ttgccatact tatatgtcgg gcattttaaa atagttactc
     1561 caatttcatc gtttggattt gcattgctca tataggttaa caaaatcttg ctccagggaa
     1621 ttccaaaatc agggctcacc ccggttccgc tataaaacgg aacaaggtat ccattgtggt
     1681 cactggcata catgaaaatt gcaagaccta tctgcttgag attgcccatg cacactccct
     1741 gcctcgcctt ctcccttgcc ttgctcaatg caggtagcag catcgctgca agtatcgcga
     1801 tgatagcaat taccacaagc aattcaatca atgtgaatcc ttttttcatt ttcaatcacc
     1861 ccctttttag ttagttttac caaatattat ctctatttgt tgcccagtcc tttcctgtgt
     1921 atggcttcac agaaccatca acaataagga aatttgcaaa tccattgtgt ctgagacaaa
     1981 tgccaaaata aggccaggat ggataaacaa cgctccagga atcattcaat ccatcaccat
     2041 caacatcaat gtcacgagtg tactgtaaag gggaaattgt gtatgtgcca tagcaatcca
     2101 caacaaggta acatgtcttt ggaagacggt caagtttcat aagttttgtc cctgcatagg
     2161 aaaacctcgc tccatccctg tagtttgctc cataagaagc catttcctgt gccgtaaaat
     2221 atttcctgta catcttgaaa ctcggacatc gcaaaaatgt gagacctata atcccggtga
     2281 ccggtaccac aagatttatt ccatattcat cagcaagaat cttgtaccag taattggtag
     2341 gatgcacatt tgtagtggaa cctgcgttaa acggcacaag tattccatcg tggtcattag
     2401 catacatgaa aattgcaaga cctatctgct tgagattgcc catgcacact gcttgcctcg
     2461 ccttttccct tgccttgctc aatgcaggta gcagcatcgc tgcaagtatc gctatgattg
     2521 caattaccac aagcaattca atcaatgtga atcctttttt cattttcaat cacccccttt
     2581 ttaccagtca atccctgttc tcgttaccca atcccttgat ttcaatggct tcacagaacc
     2641 atctgcaaaa agaaagtttg caaaccctcc atgcctgaaa cacaacccat agtgagccca
     2701 ggtaggcagg cttgcactgt acgaatcttt cacaccatcg ccgtcaatat cagtgtctat
     2761 agtcggaaat tgcaaaggcg tgttcgtata acgatttgtt gcatcagcaa ccaaccagag
     2821 atttttcggc aacctgtcca gtttcaatgt atagtttcca ctgtaacaaa atctcgtatt
     2881 tggcgctgcg ttgttacagt aattcacacc ataagaatag tacaccatgg ctccataata
     2941 cttgtcacca tatttataag ttggacattt caggaatgtt tgtccgatta tacctgattt
     3001 tccaatataa cttgcaataa tgtcgtacca gtacacagaa gacggagtag tggatgtacc
     3061 tgtgttgaat ggcacaagta ttccattgtt atcactggca tacatgaaaa ttgcaagacc
     3121 tatctgcttg agattgccca tgcacactgc ttgcctcgcc ttttcccttg ccttgctcaa
     3181 tgcaggtagc agcatcgctg caagtatcgc tatgattgca attaccacaa gtaattcaat
     3241 caatgtgaat ccttttttca ttttcaatca cctcctttgc actgtttctt ttattagacc
     3301 tgcgtttatt ctctgttcta atttttcttt tattatgaaa tccggttgaa tcacaatctg
     3361 tgatatggtc tcttgctttt taataatact ttccattttt tcaatcgcaa tctttcccat
     3421 ctcatcaaat ggctgaatta tttcgtggat tgcttccttt tcttcaataa tcccgggtat
     3481 ctcatcatag gtcaccacct ctatatcatc cgtatactgc tccccattaa gggtagatgg
     3541 aaaataaggt agaatagagt ggtctg
//
LOCUS       DAORYU010000151         3566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_17480_length_3566_cov_4.587297, whole genome shotgun sequence.
ACCESSION   DAORYU010000151 DAORYU010000000
VERSION     DAORYU010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17480_length_3566_cov_4.587297"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2236)
                     /locus_tag="PLW07_05375"
     CDS             complement(<1..2236)
                     /locus_tag="PLW07_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49990.1"
                     /translation="MKKLLMFLLFAGSCFVFADTNAYYRDVDNDGKNDVVMMENEFFR
                     ITFSPSAGAKATEVFYKPENKLMSTTSGWFQDVVVELGEMVGANLYNCTHSYESEIVK
                     TGPDFCCVTFRSNLPGTGKFENYRNITIEKTYTLSKNSPVINVGIQVKNNSKEALPFT
                     LMPAHWAWVDGEDSWYFVPDELGILNDFDSQVRTFSAPVGSQDPTSNFVGFLSTQSKL
                     GLIFVMEWKYLDAIECWLSKGKGACVQWPYRKQIVKAGQTWTTQYIIYPVSGMESMDA
                     ASEHYAIGMTVGEKSGIGNFISKEEMQIGRQVPVKIYVSSIAEGKVTIEYGYRILPDE
                     KVTILGSTQTQVEKARSTGLQFLLPIEKKDCTYVITVRLKDEKNNILQAERPVKIGDS
                     AYTYFMKPKAEQQLGEKFYGYNIINPPLPSWYEKVDLTIETPHVKWAKPWSNGKLNVL
                     FVNRSDNSVGYWREIWERCDIDFDACSLAWDTAKKFPYTQNTLKKLLRQLAEKNYDVI
                     FFSALHWDQGFPDYIKDSIFSFVKQGKGAVILADLRNKQIYGDLGKYLKENGRELDSS
                     WIIAGIPYRMPKVWLYELGKGRIVVIEGTPASGYETVSLSLGDWQADGRWMWIPGWEY
                     GFGLFSRGIIWAGKKESGIVIREMKGNEEKVEMSIDNSTGKKIDAVCSLKIYNGFYEC
                     EENSEVKVSLVPGENRIELKIAKPLTDKVHIADLIVKDGKGKNLGWASCKFTVTRDIN
                     LTAKM"
     gene            complement(2268..3458)
                     /locus_tag="PLW07_05380"
     CDS             complement(2268..3458)
                     /locus_tag="PLW07_05380"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOL49991.1"
                     /translation="MIKIGIVGTGDRGTSFVKAAEHIPEMKIEAVVDTNPIRMEAVIK
                     HHHYPPVKTFNNLDDLLDARIVDVIFITTPDWTHSDLIIKCLEKGYHVFCDKPLAINP
                     EQIQKILVAANKSGKMQFMGFNMRYNPLFTKLKEVTLNKDIGDILTGWCYSGYQGDGY
                     FRRWHKFREKSGGLIVHKGCHILDILNWMVDSYPVEVYAVGGQKVFGGNKKWPGCHEC
                     KETETCAYARKLTDDQMKLLEDIYIKAAAVDGYTRNYCVFGPSDVPDHYLLIINYANG
                     ARVIFEEIFFGTGLFYMGFAGEKGEIVTGSFIPDSIVVRRKFDQEKEKRYQVQKETKE
                     FHGGADIYMLKDLVKSIDTGNQILPGFKEGARSAIIGVAAMISIEQRKVVKIDELLPL
                     ELLQ"
BASE COUNT          952 a          851 c          584 g         1179 t
ORIGIN      
        1 gcatctttgc agtcaggttt atgtccctgg tgactgtaaa cttgcaactt gcccatccaa
       61 gattttttcc tttcccatcc tttactatca ggtcagcgat gtggacttta tcagtcaggg
      121 gctttgctat cttgagttct attctgtttt cacctggaac cagagatacc tttacttcac
      181 tgttttcctc gcactcataa aatccgttat aaatcttcag agaacacact gcgtctattt
      241 tcttgcctgt gctgttatca atgctcatct ctactttttc ttcattaccc ttcatttctc
      301 ttatgacaat gccagattcc ttttttcctg cccagatgat accacgtgaa aaaaggccaa
      361 aaccgtattc ccagccaggt atccacatcc atctgccatc tgcctgccag tcgcctaaac
      421 tgagactgac ggtttcatat ccgctggctg gagtcccttc aataacaaca atccttcctt
      481 taccaagttc atacagccag acctttggca ttctgtacgg gatgccagca ataatccagg
      541 atgaatcaag ttccctgcca ttttctttaa gatattttcc caggtccccg tagatctgtt
      601 tattcctcaa atcagcaagt attacagcac ctttcccctg tttgacaaaa gaaaaaatac
      661 tatcctttat ataatctgga aaaccctggt cccagtgcag ggctgagaaa aaaatcacat
      721 cataattttt ttcagcaagt tgccgtaaaa gttttttcaa tgtgttctgg gtgtacggga
      781 atttttttgc tgtatcccag gcaagggaac aggcatcaaa atcaatatca cacctttccc
      841 atatctcccg ccagtatccc actgaattat cacttctatt tacaaacagt acattcagtt
      901 tgccattgct ccacggcttt gcccatttaa catgcggggt ttcaatggtc aggtccacct
      961 tttcatacca tgaaggcaac ggcggattga taatgttata accatagaat ttttctccaa
     1021 gttgttgctc tgcttttggt ttcataaagt aagtatatgc tgaatcgcca attttcaccg
     1081 gcctttctgc ttgaagaatg ttgtttttct catccttcaa tctcactgta attacgtagg
     1141 tgcaatcctt tttttctatc ggcagtaaaa attgcaaacc agtacttctg gctttttcaa
     1201 cctgcgtctg tgtgcttcca agtatcgtta ctttttcatc aggcaaaatc ctgtaaccat
     1261 attcaatcgt tactttccct tcagcaatgg atgaaacata tatttttacg ggtacctgtc
     1321 ttccaatctg catttcttct tttgaaataa agttaccgat acctgatttt tctcctactg
     1381 tcatccctat tgcatagtgt tcactggcag catccatact ttccatccct gaaaccggat
     1441 aaatgatgta ctgagttgtc catgtctgac cggctttgac tatctgtttt ctatatggcc
     1501 actgaacgca tgctcctttt ccttttgata accagcattc tattgcgtca agatacttcc
     1561 actccattac aaatatcaat ccgagtttac tctgagtact caaaaagccc acaaagtttg
     1621 aagtgggatc ctgagaacca acaggagcac tgaatgtcct gacctgactg tcaaagtcgt
     1681 tcaatatccc gagttcatca ggaacaaagt accagctatc ttcaccatct acccatgccc
     1741 agtgggcagg catcagtgta aatggcaggg cttcttttga attgtttttc acctgtatcc
     1801 caacattgat cacaggagaa ttttttgaaa gggtataggt tttttctatt gtaatatttc
     1861 tgtagttttc aaattttcct gtccctggca gatttgaacg aaatgttaca caacaaaaat
     1921 ctggtcctgt tttcactatt tcactttcgt aagaatgggt gcagttgtaa agatttgcac
     1981 ccaccatttc tccaagttca acaacaacat cctggaacca gccacttgtc gtgctcatca
     2041 atttgttttc tggtttgtaa aaaacctctg tggcttttgc tcctgcagaa ggagaaaatg
     2101 taatcctgaa aaactcattc tccatcatta cgacatcgtt ttttccgtca ttgtcaacat
     2161 ccctgtagta ggcatttgtg tcagcaaaaa caaaacaact accagcaaac aaaagaaaca
     2221 ttaacaattt tttcatcatc cctccgggtt atgcgggaaa atgttgttca ttgcaatagt
     2281 tcaagcggta acagttcatc tatttttaca acttttcgtt gttcaatgga aatcattgca
     2341 gcaacaccta tgattgctga ccttgcgcct tccttaaacc caggcagtat ctgatttcca
     2401 gtgtcaattg atttcaccag gtctttcagc atataaatgt ctgctccacc gtgaaattcc
     2461 tttgtctctt tttgaacctg atatcttttc tctttttcct ggtcaaattt cctgcgaaca
     2521 acaatagaat caggaataaa agaacctgta acaatttctc ctttttcacc agcaaacccc
     2581 atataaaata gacctgtgcc gaaaaatatc tcttcaaaaa tcacccttgc gccgtttgca
     2641 tagttgataa tgagaagata atggtcaggt acatctgaag gtccaaaaac acagtaattt
     2701 cttgtatagc cgtcaacagc agcggctttt atgtaaatat cttcaagaag tttcatctga
     2761 tcatcagtta attttcttgc ataggcgcat gtttctgttt ctttgcactc atgacagccg
     2821 ggccatttct tattgccgcc aaaaactttc tgcccgccta cagcatagac ctcaacagga
     2881 tatgagtcaa ccatccagtt taaaatatca aggatgtgac agcccttgtg aacaataaga
     2941 ccaccgcttt tttctctgaa cttatgccat cttctgaaat aaccatctcc ctggtatccg
     3001 ctgtaacacc agccagtcaa aatatctcca atgtctttgt ttagagtgac ttccttcaat
     3061 ttagtaaaaa gagggttgta tctcatgtta aatcccataa actgcatttt gccacttttg
     3121 tttgcagcaa ccagaatttt ttgaatctgc tctggattta tcgcaagggg cttgtcgcaa
     3181 aaaacatggt accctttttc aagacatttg ataatcaggt ctgaatgagt ccagtcaggg
     3241 gttgtgataa aaataacatc aacaatcctt gcatcaagca aatcgtccag attattaaat
     3301 gtttttactg gcgggtaatg atggtgtttt atcactgcct ccatacgaat cgggtttgta
     3361 tcaaccactg cttcaatttt catttccggt atgtgttcag ccgccttcac gaaacttgtg
     3421 cccctgtctc cagtcccaac aatgcctatt ttaatcattt tttcctttcg tttgttgttc
     3481 actctctatt ttttcgcatt tgttatttct gcacacagtg ttctgaccga caataacaat
     3541 atctgccacc ttatgacctg aaagaa
//
LOCUS       DAORYU010000152         3516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_17792_length_3516_cov_4.387460, whole genome shotgun sequence.
ACCESSION   DAORYU010000152 DAORYU010000000
VERSION     DAORYU010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17792_length_3516_cov_4.387460"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            206..1021
                     /locus_tag="PLW07_05385"
     CDS             206..1021
                     /locus_tag="PLW07_05385"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOL49992.1"
                     /translation="MNKSKIGVIVGAGENFDQIFQRVKMLDFSLCQIGFVAENMGMFN
                     PKTIRQKADEYGIEIFSVFLLFKGQIFNLKDGPATMGFVAPDFRSQRLKLALEFSDFA
                     REIGTKHIVCHVGFIPDDELDPLYVSFIPVMRKVVEKCRDNGQNFCFETGQELPSTLK
                     RTIIDIGLDNTGINLDPANLILYGKANPVDAVEIFGQYVRSIHGKDGLWSNRDEYLGK
                     EVPVGEGMVNFPIIIRRLKKMGFQGPVIIEREISGERQIQDIKKAVEFFSMLL"
     gene            complement(1018..2298)
                     /locus_tag="PLW07_05390"
     CDS             complement(1018..2298)
                     /locus_tag="PLW07_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49993.1"
                     /translation="MAAQKTIQTNIPVLTSCPSDPDETWNVFLRNHKENPAGIWKKYG
                     RGKICIISVSQGIWHKNCLGHTAGFDSIFWRSLILTAKKPFVFKAVPPFVTCRINDAT
                     GSGHFEQSPANQFRYVSILNESGFTPHVGLCINQVNQESTSHIREFYHQSKAEFSAHA
                     FTQDFHEKDPSIYMGSDGKEFSFEQLNTHFEQIDSFFNHLNIKPAKIINAHRSQIGYN
                     AISFFKQRQQTFSASFLKPGKIFSDPRGLLWEPKPFGIINFCIDYLDENQDIFNVVSN
                     PGRITEKGADIDFLDTATSILSAVENGVFQIKRGIENLSFGCLMFHEKNCEKLAFSDF
                     EMTIGMITNDIKKMPHIFKPYDYIVAYLKNRLDSKIVSMTHTNSHLSITLSGKSTIPQ
                     FFYCFLEDGTNIVQNFLEIPPFEKAIELTYKIAI"
     gene            complement(2302..2667)
                     /locus_tag="PLW07_05395"
     CDS             complement(2302..2667)
                     /locus_tag="PLW07_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49994.1"
                     /translation="MKTVVLCDSSDFVSYQRFENSILNALDFYQIWYQSIDISYTLVD
                     SVELDDVNLLIIAQEGTARKLGPSDWRLIFKQVSAGMGLVIFDGTLNTYQEALSPYTG
                     ITRFNIEKTGSFSIEDGWI"
     gene            complement(2670..3179)
                     /locus_tag="PLW07_05400"
     CDS             complement(2670..3179)
                     /locus_tag="PLW07_05400"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOL49995.1"
                     /translation="MNSKIIKEIAQATKNIPEHYIDEIIKLLDTSDRIFVWGLGRSGM
                     MARAFAMRLRHLGRESYFIGGLCPTMQNKDLLIVISKTGRSKMLWVPVEAAKKSKTTT
                     IYLTATKNPLTKLCDRSFYFNLPESSQFGGSLFEQTMLIFLDELVERYRQTHGISFTK
                     MEKNHASWE"
     gene            complement(3269..3391)
                     /locus_tag="PLW07_05405"
     CDS             complement(3269..3391)
                     /locus_tag="PLW07_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL49996.1"
                     /translation="MRRNLKGGDLEKVFCKGGERKKEFEKGEDKRRRFCISREN"
BASE COUNT         1004 a          671 c          690 g         1151 t
ORIGIN      
        1 tacaaattcc tcctccaggt ctcccccttt tcaaaagggg gattaagggg gatttcataa
       61 aggaagtcga cctgaagttg gcggggactc ataaaggaag aatccctgaa acaggcaggg
      121 tacggttctt gcgaggaaat tttttatcgt ttatcattgt tctgtcgcag aacaaatttt
      181 cacaattttt actggagaca ggtttatgaa taaatcaaaa attggtgtaa tcgttggagc
      241 aggcgaaaat tttgaccaga tttttcaaag agtgaaaatg cttgatttct cattgtgcca
      301 gataggtttt gtggctgaaa acatgggcat gtttaacccg aaaacgatca gacagaaggc
      361 agatgaatac ggaattgaaa ttttttctgt ttttcttctt ttcaagggac agatttttaa
      421 cctgaaggat ggccctgcga caatgggttt tgtggcgcct gacttcaggt cacagagatt
      481 gaaacttgct cttgaatttt ctgattttgc aagggaaata ggaacaaaac acattgtctg
      541 tcatgttggt tttattcctg atgatgaact tgaccccctt tatgtttctt tcattcctgt
      601 gatgagaaag gttgttgaaa agtgcagaga caacggtcaa aatttctgtt ttgaaacagg
      661 ccaggaactt ccatcaacgc tgaaaagaac gattattgac attggtcttg acaacactgg
      721 tattaacctt gaccctgcca atttaattct ttacggaaag gcaaatccag ttgatgctgt
      781 tgagatattc ggtcaatatg ttagaagcat acatggcaag gatggactct ggtccaatag
      841 agatgagtat ttgggtaaag aagttcctgt tggcgaaggt atggtgaatt ttcccatcat
      901 tatccgaaga ttgaaaaaga tgggatttca gggacctgtg attattgaaa gagaaatatc
      961 aggtgaacgc cagattcagg atatcaaaaa ggcagttgag tttttttcaa tgctccttta
     1021 aattgcaatc ttataggtca attcaatggc tttttcaaac ggcggtattt caagaaaatt
     1081 ttgaacaatg tttgttccat cttcaagaaa gcagtaaaaa aactgtggaa tcgtgctttt
     1141 ccctgatagt gtaatagata gatgggaatt tgtgtgcgtc atgctgacaa tttttgagtc
     1201 aagacgattt ttaagatatg ccacaatata gtcatatggt ttgaaaatgt gcggcatttt
     1261 ttttatgtca tttgtaatca ttccaatcgt catttcaaaa tcagaaaaag caagcttttc
     1321 acagtttttt tcgtgaaaca tcaaacagcc aaaactcaag ttttcaatgc ctcttttaat
     1381 ctgaaatact ccattttcaa ctgctgaaag aattgaggtt gctgtatcca gaaaatcaat
     1441 atcagcgccc ttttcagtga ttctaccagg atttgacaca acattgaaaa tgtcctggtt
     1501 ttcatcaaga tagtcaatgc agaaattgat aatgccaaat ggttttggtt cccacaaaag
     1561 tccacgtggg tcagaaaaaa ttttacctgg tttcaaaaaa ctggctgaaa aagtttgctg
     1621 gcgttgtttg aaaaaactga tggcattgta tccaatctga gacctgtgag cattgataat
     1681 ttttgctggt ttgatgttga gatgattgaa aaaactgtct atctgttcaa agtgggtatt
     1741 gagttgttca aaactgaatt cctttccatc gcttcccata taaattgaag ggtctttttc
     1801 atgaaagtcc tgcgtaaatg catgggctga aaactctgct tttgactggt gatagaattc
     1861 cctgatgtga gacgttgatt cctgattgac ctggttaatg cataacccta catgtggagt
     1921 aaaacctgat tcattcagta ttgaaacata cctgaattga ttggcagggc tctgttcaaa
     1981 atgacccgaa ccagttgcat cattgattct gcaggtaaca aatggtggaa ctgccttgaa
     2041 aacgaatggt ttttttgctg taagtatcag agacctccag aagatactgt caaagccagc
     2101 agtatgaccc agacaatttt tatgccagat tccctgagaa acactgataa tgcaaatttt
     2161 ccccctgcca tattttttcc agattccagc agggttttct ttatgatttc tcaaaaaaac
     2221 attccaggtt tcgtcagggt ctgaagggca actggtgagc acaggaatat ttgtctggat
     2281 ggttttttgc gctgccatgt cttaaatcca gccatcttcg atggaaaaag aaccggtttt
     2341 ttcaatgttg aaccgtgtaa tacctgtgta cggggatagg gcttcttgat aggtgttaag
     2401 tgttccgtca aaaatcacca gccccattcc ggctgagacc tgtttaaaaa taagtcgcca
     2461 gtcagatggc ccgagttttc ttgctgtgcc ttcctgggca atgatgagta aatttacatc
     2521 gtcaagttct actgaatcaa caagggtgta agatatgtct attgattgat accagatttg
     2581 atagaaatcc agagcattca ggattgaatt ttcaaaccgc tgataactga caaaatcaga
     2641 actgtcacac aggacaactg ttttcatact tattcccagc ttgcgtggtt tttttccatc
     2701 tttgtaaacg aaataccatg agtttgcctg tatctttcaa caagttcgtc aagaaaaatc
     2761 agcatggttt gttcaaatag ggacccacca aactggctgc tttcaggaag attgaaataa
     2821 aatgacctat cacacagttt tgtcaaagga ttttttgtcg cagttaaata gatggttgtt
     2881 gtttttgatt tttttgcagc ttcaacagga acccagagca tttttgacct tccagttttt
     2941 gaaattacaa tcaacaggtc cttgttttgc attgtcgggc acaacccgcc gataaaataa
     3001 ctttccctgc caaggtgtct caatctcatc gcaaaagcgc gtgccatcat tccgcttcgt
     3061 ccaagccccc atacaaaaat cctgtcactg gtatcaagca gtttgatgat ttcatcaata
     3121 taatgttcag ggatattttt tgttgcctgc gcaatttcct ttatgatttt gctattcata
     3181 atgtttattt taattccatg tggtattttc cgcaattttt tgatgtcaac aattttttgt
     3241 tgcatttctg gtctttacaa aatttccttc agttttctct acttatacaa aatctccttc
     3301 ttttatcttc ccccttttca aattcttttt ttcgttctcc ccctttacaa aataccttct
     3361 ccaggtctcc ccctttcaaa tttctcctca taatttcccc ctttacaaaa ttccttcctc
     3421 catatccccc ttttcaaaag ggggacaaag ggggatttca agaaatctcc cccagcccct
     3481 cttttataaa gaggggagtg atttttctcc ctttat
//
LOCUS       DAORYU010000153         3514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_17809_length_3514_cov_3.712634, whole genome shotgun sequence.
ACCESSION   DAORYU010000153 DAORYU010000000
VERSION     DAORYU010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17809_length_3514_cov_3.712634"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="PLW07_05410"
     CDS             <1..598
                     /locus_tag="PLW07_05410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOL49997.1"
                     /translation="MFVAEIACLPEYGNIIVPVSATSKKHAEKFAEKHNLKGKKVLGA
                     VIGTGNRWITKRWPEKHFLDLFSSLDDFEILIFGGSEEKDLLHQIAKKSDKRVINTGY
                     DNSIDLFFGLLDLCDVVLCCDTFALHVAVGLGKKTVAMFGPTSSNEIEMYDNGVKIVS
                     PATCICCYKKRCSRNPTCMELITPQQVEQSIRRYCFER"
     gene            588..1298
                     /locus_tag="PLW07_05415"
     CDS             588..1298
                     /locus_tag="PLW07_05415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOL49998.1"
                     /translation="MKDSIALIIPTYNERENIIPLVQEILGLNMNIKVIIVDDNSPDG
                     TGIIVREFFKDNQHIKVFIRTEKRGRGYAGRFGYKMALQENCDVIGEMDGDFSHSPEF
                     IPGMVECLKFADAVSGSRFLSGAMSLRKNFLREMVTRCARIYLKLILGIKLTDPTSGF
                     RFFKSSLLSEIFPLLKSRDAFIVTEVFYYTFKKKLRVKEYPIVFYERKYGRSKLKIST
                     LVAYLYRVLLLRTGYFNG"
     gene            1291..3393
                     /locus_tag="PLW07_05420"
     CDS             1291..3393
                     /locus_tag="PLW07_05420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOL49999.1"
                     /translation="MDEKKLWQRAYLLIGISFIIRLLIAAFYGLSGDEAHYYQYAMHP
                     SMSYFDHPPLVGYAILPFLKIFGNTPLAVRLPALISSTLCMWLLLKSGKDLYSTVTGF
                     WAILIFCFIPLFSVLGGIMTVPDTILSVFWLLSVMTIWKIRTTGKDNLWYLVGIFTGL
                     ALLTKYSAFLIYPGILLFLLINKEMRNSFKKKQLYLGFILSILIFLPVLVWNYNNYWV
                     SFAFQFKHGLGEKSFFKPGIFIHNIVSQMLLISPLVWFMLVISFFKIAVQSFFHKHDP
                     AQLFFSFSLPVFIVFGYASFSNEILPHWPAVGYLMLIVPCAFFLQTIFIGTSRIKKSI
                     AKFSLFTGLLFTMVIPVQVVFHCFPLSPETDPTGDLYGWDIGARVAECLVSRNQNNFL
                     FTHKFYLASQMAFSLPVDVANNRLFCLSKRVDQYDFWQRDRNLKTELSGENGIFFTDE
                     HFKDDPGKMYGFETVDRPITVEIFYKGKKVKTFRFYQCYNFKTSETDPLYFNSLNFKQ
                     RSFFKKVIEIDNRAFLTVNKFAFRNKWLANILFAIGYLGSTEVASLIVVVLLYFTRRS
                     ELVRYFWIFLSMLIFGAIIVHIIKELFPSARPVSYFKDHVICVTGPVLKSDSFPSGHA
                     QTIFTAAFFLSWIFRKLWPVFFAFAFMVAFSRVFARVHFFRDILCGSIIGLISFWVVL
                     KIYLTIFKKTEAEKFDNK"
BASE COUNT         1007 a          578 c          686 g         1243 t
ORIGIN      
        1 aatgtttgtt gcagaaattg cgtgcttgcc agagtatggg aatattattg ttccggtgtc
       61 tgccacatca aaaaaacatg cagaaaaatt tgcagaaaag cacaacctga agggtaaaaa
      121 agtgctggga gcagtaattg gaacaggaaa cagatggatt acaaagagat ggcctgaaaa
      181 gcattttctg gatttgtttt catcccttga tgattttgag attcttattt ttggtggttc
      241 tgaagaaaag gatttacttc accagattgc taaaaaaagt gataagaggg ttattaacac
      301 aggatacgac aattccattg atttgttttt tggtttgctt gatttgtgtg atgtcgtttt
      361 atgttgtgat acgtttgctc ttcatgtagc agtcgggctt ggtaagaaaa ctgtcgcaat
      421 gtttggacca acatcctcaa atgagattga gatgtacgat aatggggtga aaattgtcag
      481 tcctgctacc tgcatatgtt gttacaaaaa aagatgttca agaaacccaa cgtgtatgga
      541 attaataaca ccgcaacagg ttgaacaatc catcaggaga tattgctttg aaagatagca
      601 tcgctttgat tattcccacc tacaatgaaa gggaaaatat cattccactg gtacaggaaa
      661 tacttggcct taacatgaat atcaaggtga tcattgttga tgataattct ccagatggta
      721 cgggtattat tgtcagggaa ttttttaagg ataatcaaca tataaaagtt tttataagaa
      781 cagaaaaaag aggaagaggg tatgctggaa ggtttggtta taaaatggca ctacaagaaa
      841 attgtgatgt tattggagaa atggacggcg atttttctca ttctcctgaa tttattcccg
      901 gaatggttga atgtcttaaa ttcgcagatg ctgtgtcagg gtcacgattt ttatcaggag
      961 caatgagttt acgcaaaaat ttcttaagag agatggtaac acgatgtgca agaatttacc
     1021 tgaaacttat tcttggtatt aaactgacgg atccgacttc cggcttcaga ttttttaaat
     1081 catctttgct atcagaaata ttcccactcc ttaagtcaag ggatgctttt attgtcacag
     1141 aagtattcta ctatacgttt aaaaaaaaat tacgagtcaa agaatatccc attgtctttt
     1201 atgaaagaaa atacggcagg tctaaactaa aaatctccac acttgttgcg tatctctaca
     1261 gggttttact tcttcgtaca gggtatttca atggatgaaa aaaaattatg gcagagggcg
     1321 tacctgttaa tagggatatc atttattatt agattattga tagctgcttt ttatggtttg
     1381 agcggggacg aagcccatta ttatcaatat gctatgcatc cttcaatgtc atattttgat
     1441 catccaccac tcgtgggtta tgcaatctta ccatttttaa aaattttcgg aaatactcca
     1501 cttgcagtga gattgcctgc tttgatttca tctacactat gtatgtggtt acttttgaaa
     1561 tcaggaaaag atttatattc aactgtaaca gggttctggg ctatcctcat cttttgtttc
     1621 attcccctgt tttctgttct cggtggtatc atgacagtac cggacacaat tttgtctgtt
     1681 ttctggttgt tatctgtcat gacaatatgg aagattcgta ccacaggtaa agataacctc
     1741 tggtatcttg ttggaatttt cacaggactg gctttgctta caaaatactc ggcatttcta
     1801 atatatcccg ggattttgct ttttttgtta atcaacaaag aaatgagaaa ttccttcaaa
     1861 aaaaaacaat tatatctggg atttattctc agtattttaa tatttcttcc tgttctggta
     1921 tggaattaca ataattactg ggtgtcattt gcatttcagt tcaaacacgg tcttggtgaa
     1981 aaatctttct ttaagccagg tatttttatc cacaatattg tttcacagat gttgctgata
     2041 tcgccacttg tgtggttcat gctggtaatc tcatttttta aaattgcagt tcaatctttt
     2101 tttcataagc acgatccagc acaattattt ttcagttttt cgttacctgt ttttattgtt
     2161 tttggttatg catccttttc aaacgagatt ttgccacact ggcctgcagt tggttatctc
     2221 atgcttattg taccgtgcgc tttctttctg cagacaattt ttatcggaac aagccgaata
     2281 aaaaaatcta ttgcaaaatt ttctttgttc acaggacttt tatttacaat ggttattcct
     2341 gttcaggttg tttttcattg tttcccttta tctcctgaaa cagatcccac aggtgatctg
     2401 tatggatggg atattggtgc acgggtggcg gaatgccttg tttcacgtaa ccagaataat
     2461 ttcttgttta ctcacaagtt ttatcttgca agccagatgg ctttttctct cccggtggat
     2521 gttgcaaata atagactttt ctgtttgagt aaaagggtgg accagtatga tttctggcag
     2581 agagatagaa accttaaaac agaactttcc ggtgaaaacg gtattttttt tactgatgaa
     2641 cattttaagg atgatccggg aaaaatgtac ggatttgaaa cggttgacag acctattact
     2701 gttgaaattt tttataaagg gaaaaaggtt aagacatttc gtttttacca gtgttacaat
     2761 ttcaaaacca gtgaaacaga tcctttatac tttaattcct tgaatttcaa acaaagaagt
     2821 ttttttaaaa aggtcattga gattgataac cgtgcttttc tcacagtcaa caaatttgct
     2881 ttcagaaata aatggcttgc aaatattcta tttgctattg gttatcttgg gtcaacggaa
     2941 gttgcttcac tgattgtcgt cgtgcttctt tatttcacaa gacgcagtga actggtgcga
     3001 tatttctgga tttttctttc tatgttgata tttggcgcca tcattgtcca tattatcaag
     3061 gaactgtttc cttctgcgag gcctgtatca tatttcaagg atcatgttat ttgtgttacc
     3121 ggtcctgttt taaagtcgga ttcatttcct tctggccatg cgcaaacaat ttttactgct
     3181 gcattttttt tgtcctggat atttcgtaaa ttgtggccgg tatttttcgc ctttgctttc
     3241 atggttgcct tttcaagagt ttttgcaagg gttcattttt ttagagatat tttgtgtggg
     3301 tctatcatag gattgatatc tttctgggtt gtgttgaaaa tttacttaac tatttttaaa
     3361 aaaactgaag cagaaaaatt tgacaataaa tagaactgat gtatcatatt tcactatcaa
     3421 aaaattgaaa cttcccgtaa ataactcgcg ctaaaattac gcgggattaa ctcagcggta
     3481 gagtgcgacc ttgccaaggt cgaagtcacg ggtt
//
LOCUS       DAORYU010000154         3499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_17920_length_3499_cov_3.258711, whole genome shotgun sequence.
ACCESSION   DAORYU010000154 DAORYU010000000
VERSION     DAORYU010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17920_length_3499_cov_3.258711"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..522
                     /locus_tag="PLW07_05425"
     CDS             145..522
                     /locus_tag="PLW07_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HOL50000.1"
                     /translation="MYCKNCGKEVSEQAAVCVVCGISPKKGNKFCFNCGTPVNPQAEI
                     CTKCGVKLMTSNNEGKDWLVVVLLSALVGQFGIDRFYLGYTGLGILKLLTLGGCGIWW
                     LIDLILVATNNIKDANGNPLVKK"
     gene            509..826
                     /locus_tag="PLW07_05430"
     CDS             509..826
                     /locus_tag="PLW07_05430"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HOL50001.1"
                     /translation="MSKNRIAEFLALGCPAARVIVMSAGMIFLACVNLESLRIPDLCI
                     WEKIFGYCPAHGTTRALSAFFHGRFCEATRHNLNVLFIVPTLAGIILTDVVRLIKRNL
                     EKI"
     gene            complement(925..2859)
                     /locus_tag="PLW07_05435"
     CDS             complement(925..2859)
                     /locus_tag="PLW07_05435"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOL50002.1"
                     /translation="MNKTLWQYYVDKASEITDNALNHIKTLKQWENEIEQRKKEFFIS
                     MGLNPLPEKCDLSISIIGTLRKKGFIAKKISYQILPDCYSSGILFLPDPLPSKKVPAV
                     LYCCGHSGLGVHWYQKHAITWAKRGYACFIFETIRQSENPGYHHGLFTGKRLDWISLG
                     YTAAGGELLNSMCALDVLCEMPEIDKNRIGATGISGGGAHSHFLAIADERIKAVATSC
                     GISLLKFAIGYRTMFQHCDCMYANGNFQRDGSEFSALIAPRPLLYCFASEDNLFVHDE
                     YRTMYQKVKHIYRLYGCEEKCELFEYPGPHAYSSESVKKINEWFDRYVAGEQHPDIGL
                     DSETIAESEISVLNGKTPEPDRLDILPELLTIKKTYPLPVDKNEWNTLRNEIVENLKK
                     NIFHWLDRSNEKMEIKKIGHQGQWIWENNYFSRYVAKSGGMESYIEVFSKKNPAQNTI
                     IVLGDYKQNTQTLLNKTGLLTEKFNLILVEPRACGRNAADMEQGNHCLYRAGVMTGIT
                     PIMMWMNDMKYVVEFFKNTDAFAKTKFVLFGTGEAAGACLYYSIFDESISAVILDSVV
                     DTHAGGCYIPGILKDIDITDASGLMAPRVFAVCVVNMDRWFLKMHWGVRVYDRLGIKE
                     KYIMVQNTNTAIEKVLSEIS"
     gene            3058..>3499
                     /locus_tag="PLW07_05440"
     CDS             3058..>3499
                     /locus_tag="PLW07_05440"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50003.1"
                     /translation="MEKFYKRVPARVNPLLRHKDEKSFDLGGQWLFTLDPQDKGIKEK
                     WYVNKEVFSAKISVPGSWQGQGCGDESLDKIWDFRLKARVYRATYHGTGWYGKIFRIP
                     QGWENKKIWIYFGGVHPSCEIWVNGKYIGSHSGPFVPFAFDITDV"
BASE COUNT          984 a          689 c          713 g         1113 t
ORIGIN      
        1 gggaagcaaa agatgtgatt ttgatgaaaa atgttccttc tgaaatatta ccttacatta
       61 tcctggcgcg actggttctg tttgctctac tggttggaat tgcatggctt gttcacattg
      121 cctggaagaa aaaggaggca taacatgtac tgcaaaaatt gtggtaagga ggtatccgag
      181 caagcggcag tatgcgttgt ttgcggtatt tcaccaaaga aaggaaacaa attctgcttt
      241 aactgcggta cacctgtaaa tcctcaggca gaaatctgta caaaatgcgg ggtgaaactt
      301 atgacttcca ataacgaagg caaggactgg ctggttgttg tgttactttc ggctcttgta
      361 gggcagtttg gcatagacag attctatctc ggttatacag ggctgggtat tttgaaatta
      421 ttgacacttg gtgggtgtgg tatatggtgg ttgattgatc tgatactggt ggcaacaaac
      481 aatataaagg atgccaatgg gaatccgctt gtcaaaaaat agaatcgcag aatttctcgc
      541 tcttggttgt cctgcggcaa gagtcattgt tatgagcgca ggaatgattt ttcttgcgtg
      601 cgtaaatctg gaatcactgc gcattcctga tttgtgcata tgggaaaaga tttttggata
      661 ttgtcctgcg catggaacta cgagggcatt aagtgctttc tttcatggaa gattttgtga
      721 agctacgagg cacaacctga atgttctttt tattgttccg actctggcag gaataatcct
      781 tacagatgta gtgaggttaa taaaaagaaa cctggagaaa atatgacacc tgcgggctgg
      841 attctgatga tatgcacctg gacttttgtt acagcacttc ttgttttctg cttatggaag
      901 atcttaaaaa ggcaatagcc attatcagga aatttctgat aatacctttt caattgcagt
      961 atttgtgttc tgaaccataa tgtacttttc tttgataccc aatctgtcgt agacccttac
     1021 cccccagtgc attttcaaaa accatctatc catattcaca acacacactg caaaaaccct
     1081 cggagccatt aatcctgatg cgtcagtaat atcaatgtct ttcagtattc cagggatata
     1141 acatcctcca gcatgggtat caacaacact atccagaatc actgctgaaa tgctctcatc
     1201 aaaaattgaa taataaagac aggcacctgc cgcctcacct gttccaaata gcacaaattt
     1261 tgtttttgca aaagcatctg tatttttgaa aaactcaacg acatacttca tgtcattcat
     1321 ccacatcatt ataggagtaa tgcctgtcat cacgcctgcc ctgtaaagac agtgatttcc
     1381 ctgttccatg tcagcagcat ttctaccaca tgcccttggc tcgacaagaa taaggttgaa
     1441 tttttctgta aggagacctg ttttattcag aagtgtttga gtgttttgtt tataatctcc
     1501 aagcacaatg atggtgtttt gtgcaggatt cttttttgag aatacttcaa tataagattc
     1561 catcccaccg gactttgcaa catatcttga aaagtaattg ttttcccata tccactgacc
     1621 ctgatgaccg attttcttta tttccatttt ctcattgctt ctatcaagcc agtgaaaaat
     1681 atttttcttg agattttcaa caatttcatt tcggagagtg ttccattcgt ttttatccac
     1741 aggaagtggg tatgtctttt ttatggtcag cagttcagga agaatgtcta atctatctgg
     1801 ttcaggcgtt tttccattta acactgatat ttctgattca gcgatggttt cactgtcaag
     1861 accgatgtca gggtgttgtt cccctgcgac atatctatca aaccattcgt tgattttttt
     1921 cacggattca ctggaatatg catgcggtcc gggatactca aagagttcgc atttttcttc
     1981 acatccataa aggcggtaga tatgtttcac tttttgatac attgttctgt attcatcgtg
     2041 aacaaaaaga ttgtcttctg acgcaaaaca atacagtaaa ggtcttggtg caatcaaagc
     2101 agaaaattcg ctaccatccc tctgaaagtt tccatttgca tacatacaat cgcaatgctg
     2161 aaacattgtt ctatatccga tggcaaattt tagtaaactg attccacatg atgtcgccac
     2221 tgccttgatt ctttcgtctg cgattgcaag aaaatggctg tgagcgccgc ctcctgaaat
     2281 tcccgtcgct ccaatgcgat ttttatcgat ttccggcatt tcacagagaa catcaagggc
     2341 acacattgaa ttaagcagtt ctccacctgc tgctgtataa ccgagtgaaa tccagtcaag
     2401 ccgttttcct gtgaataacc cgtgatgata accagggttt tcactctgcc tgattgtttc
     2461 aaatatgaaa caggcatagc ccctttttgc ccacgtaatt gcgtgttttt gataccagtg
     2521 tacgcctaat cctgaatgac cgcagcaata aagcacagca ggaacttttt ttgaaggtaa
     2581 cgggtcagga agaaaaagaa taccactgct gtagcagtca ggaagaattt gatatgaaat
     2641 tttctttgct ataaaacctt tttttcgcag cgtcccgata atactaattg aaaggtcaca
     2701 cttttcaggt agtgggttaa gccccataga aatgaaaaac tcttttttcc tttgttctat
     2761 ttcgttttcc cattgtttga gcgtcttgat atgattgaga gcattatctg taatttcact
     2821 tgctttatcc acataatact gccacagagt tttgttcata ttttcctcca cagattttga
     2881 aattctgtgt gattttatta cttttcatag acagtgtcaa tcaaacgcct tatactgctc
     2941 cccataatgg gcagcagtat cgcaacgacc cattttcgta tagaaaatta atgaggtaat
     3001 tcgaggtatt gacatgacca gaatttatca taataataaa gccaacagga caaaacgatg
     3061 gaaaaatttt ataaacgcgt tccggctcgt gtcaatccac tgctcaggca taaggatgaa
     3121 aaatcttttg accttggtgg gcaatggctt tttacacttg acccgcagga taagggaatc
     3181 aaagaaaaat ggtatgtaaa caaagaggtt ttttcagcaa agatatctgt gcccgggtcc
     3241 tggcagggac agggttgtgg agatgagtct cttgataaaa tctgggattt cagattaaag
     3301 gcacgtgttt acagggcgac gtatcatggt actggttggt acggaaaaat attcaggatt
     3361 ccccagggat gggaaaataa gaaaatatgg atttactttg gcggtgttca tccttcctgt
     3421 gaaatatggg tcaatggtaa gtatattggt tctcattcag ggccatttgt tccatttgca
     3481 tttgatatca cagatgttg
//
LOCUS       DAORYU010000155         3425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_18412_length_3425_cov_3.622255, whole genome shotgun sequence.
ACCESSION   DAORYU010000155 DAORYU010000000
VERSION     DAORYU010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18412_length_3425_cov_3.622255"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..1172)
                     /locus_tag="PLW07_05445"
     CDS             complement(39..1172)
                     /locus_tag="PLW07_05445"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HOL50004.1"
                     /translation="MEKNIKLNERFIKQLVKKYGTPLFLIQASRLKERVRLFREFLPR
                     VEPFYAMKANPQLSILKFFLKNGFGFDVASKNEIETVLKLGANPEKLIFANTVKSPES
                     LKFAQKNKVMLMTFDSEYELAKIARFCPDARVVVRIKVPNVGSIVELSLKFGAEPADA
                     IPLLIKAYKMGLKPAGVSFHVGSQCLELSNFVEALEISSIIFHDALLKKIPLEILDIG
                     GGFPIVHSDSESDIFPSMAERISTELDRLFDKNIKIIAEPGRFLVGPACVLITRVIGK
                     SIRNNKHWYYLDDGVYGCLSGIVYDHCKYQYRVFRKGPTQISTLAGPTCDSFDVISIS
                     EELPELEFGDIVYIENIGAYSIASATHFNSIPPAKTVFLNTGG"
     gene            complement(1179..2936)
                     /locus_tag="PLW07_05450"
     CDS             complement(1179..2936)
                     /locus_tag="PLW07_05450"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOL50005.1"
                     /translation="MQISGFFSRYLENNRKVSMPLLYRLFEKYGTIDNFRIAAGLKEG
                     EITRRLATDSDLYKWMEAISWDRQNYPDKKNQQLLDRLISLISKAQEKDGYIDTFYTG
                     AFKKLRFTDLENGHELYCGGHLIQSAIAHYRNTGKENFLNIAVKWADLICEKFEKKEI
                     TRNDGHPEVEMALVELYRTTGNEKYLLLAGKLMDVPYVYLGNKRFLDMKEINGHAVRM
                     LYLLCGATDYYAESGDTKYLKNAKLMYDDLCVGKYYITGGVGSRYERESFGMQYELPN
                     LRAYCETCASIALMMWLYRMFLVEPQSCYFDLFETVLYNAFLSAVSLTGKEYFYVNPL
                     ASCGTHQRRAWYKTTCCPPNFQRFMASLPGYFYAVSKNRVWVNLYDENQTDIELPSGQ
                     KIFFDIKTRYPWNGDVMINAKTIGDPVHLYLRIPSWSHRTSITCGKTVTFAKPGSYHR
                     LKISGSTNIHLKFEISCEFYMCKPAVESNRNCVSIRRGPIVYCFEDVDNKFDIFNFSL
                     PRQRFTGKFEEILGGIIALYGHGFAGRDNMSLYQTYQGLNHDNKFFSRKISVKLIPYF
                     AWANRKKSKMITWVPFYNK"
     gene            complement(3050..>3425)
                     /locus_tag="PLW07_05455"
     CDS             complement(3050..>3425)
                     /locus_tag="PLW07_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOL50006.1"
                     /translation="DVTIGYELRCAKPIPFDVEYTHQLGCGAVKYLLNKTKKGYQSGA
                     LISIQSGKIVPIVFDDILDPNTGKTAVRKVDVSADYYISARDLMIRLEKSDFEDRKKL
                     TKIASAAKCNVKEFEKKYSGVM"
BASE COUNT         1045 a          706 c          573 g         1101 t
ORIGIN      
        1 cattcaaatt tattctcacc ctatgttcac gcttcatatc aacctcctgt gttaagaaaa
       61 actgtctttg ctggcggaat actattaaaa tgcgttgctg aagcaattga gtatgcgcca
      121 atattttcta tgtacacaat atctccaaac tcaagttctg ggagttcctc acttattgaa
      181 atcacatcaa aagagtcaca tgtcggacca gcgagtgttg aaatctgggt gggccctttc
      241 ctgaagaccc tgtactgata tttacagtgg tcatacacaa tacctgatag acacccataa
      301 acaccatcat ctaaataata ccaatgttta ttatttctta tggatttgcc tataacacgg
      361 gtaatgagaa cacaggcagg accaacaagg aatctaccag gttccgcaat aatttttatg
      421 tttttatcaa ataatctatc aagttctgtg gaaattcgtt cagccatcga agggaaaata
      481 tccgattcag aatcagaatg aactatcgga aaaccaccac ctatgtcaag aatttcaaga
      541 ggaatctttt ttaaaagagc atcatgaaaa atgatagagg aaatttcaag tgcctccaca
      601 aaatttgaaa gctcaagaca ttgagagccg acgtggaaac ttactcctgc tggtttcaaa
      661 cccattttat aggcctttat caatagcgga attgcgtctg cgggttctgc tccgaatttc
      721 aaggacaatt caacgataga accgacattt ggaactttaa ttctcacaac cacccgtgca
      781 tcaggacaga acctcgcaat ttttgcaagt tcgtattcag aatcaaaagt catcaacatc
      841 actttgtttt tttgtgcgaa tttcaaagat tctggtgact tgactgtatt tgcaaaaatt
      901 aatttttcgg gatttgcgcc aagtttcaac accgtttcaa tttcattctt cgaagcaacg
      961 tcaaaaccaa aaccattctt caaaaaaaat ttcagtatag agagctgcgg atttgctttc
     1021 atggcataaa atggctcaac ccttggaagg aattcacgaa aaagtcttac ccgttctttt
     1081 aatctggagg cttgaatcag aaacagcggt gtgccatatt tcttgaccag ttgcttaatg
     1141 aacctctcat tcaattttat gttcttttcc attgtttctt atttgttata aaaaggaacc
     1201 caggttatca tttttgattt ttttctgttt gcccaggcga aatatggaat taatttaacc
     1261 gatatttttc ttgaaaaaaa tttgttgtca tgattgaggc cctggtatgt ttgatacagt
     1321 gacatattgt ccctgccggc aaatccgtgt ccgtagagtg cgatgattcc acctagaatt
     1381 tcttcaaatt ttccagtaaa acgttgccgc ggtaaactaa aattgaaaat atcaaactta
     1441 ttatcaacgt cttcaaagca gtatacaatt ggccctctcc tgatagaaac acaatttctg
     1501 tttgattcaa ctgccggctt acacatataa aattcgcagg agatttcgaa tttcaaatga
     1561 atgtttgtgc tgccagatat tttcaaacga tggtaggaac caggcttagc aaaggtaacc
     1621 gttttcccac aagtaattga tgttctatga ctccaggatg gaattctgag gtataaatga
     1681 accggatctc caatcgtttt tgcatttatc ataacatcac cattccaggg atatctggtc
     1741 tttatatcaa aaaatatctt ttgccctgaa ggtagttcta tgtcggtctg attttcatca
     1801 taaagattaa cccaaaccct atttttacta acggcataga aataacccgg caaacttgcc
     1861 ataaacctct gaaaattagg aggacaacag gtcgttttgt accatgctct cctctggtgc
     1921 gttccacagg aagcaagggg atttacataa aaatattctt tacctgtgag agaaacagcg
     1981 gaaagaaaag cattataaag aacagtttca aacaggtcaa aataacagga ctgcggttca
     2041 accagaaaca ttctgtacaa ccacatcatc aatgcaattg aagcgcatgt ttcacagtag
     2101 gcgcgtaaat ttggaagctc gtattgcata ccaaaacttt cacgttcata tcttgaaccg
     2161 acccctcctg ttatataata tttcccgacg cacaaatcat catacattag ttttgcattt
     2221 tttaaatatt ttgtgtctcc actttctgcg taatagtcgg ttgcaccaca aagaagatat
     2281 agcattcgta cagcatgtcc atttatttcc ttcatgtcca gaaacctttt gttaccgaga
     2341 tacacgtatg gcacatccat caatttaccc gctaaaagca ggtatttttc atttcctgtg
     2401 gtacgataca gttcaaccag agccatctca acttctggat gtccatcgtt tctggttatc
     2461 tctttttttt cgaatttttc acagataaga tctgcccatt tgacagcaat gttaagaaaa
     2521 ttttcctttc cagtatttct gtaatgagca atagcagact gaatcaaatg accgccacaa
     2581 taaagttcgt gcccattttc caggtcggta aaccttaact ttttaaatgc gccagtataa
     2641 aacgtgtcaa tatacccatc tttttcttga gcttttgata ttaaagaaat tagacgatca
     2701 agaagttgtt gatttttttt atcaggataa ttttgccggt cccaggatat cgcctccatc
     2761 catttataaa gatcagaatc agtggcaaga cgtctggtaa tttctccttc tttaagacca
     2821 gcagcaattc taaagttatc aattgttcca tacttttcaa acaatctata caaaagtggc
     2881 atgctgactt ttctgttatt ctcaaggtaa cggctgaaaa atcctgatat ttgcacgctt
     2941 ttaattgaaa caggaaaaac cttcagtttt gtatacctgc aaggtgtatt atcatctttt
     3001 gtcaggtaac gctaagttca tgtgtaatgc tcaggtaaat gaaatccaat tacataaccc
     3061 cagaatattt tttctcaaat tccttgacat tacattttgc cgcagatgca atttttgtca
     3121 gtttttttct atcttcaaaa tcggattttt caagccttat catcaggtcc cttgcagata
     3181 tgtaataatc tgcagaaaca tcaaccttcc ttacagcagt ttttcctgtg ttcggatcaa
     3241 gaatgtcatc aaaaacaatt ggaacaatct tacctgattg aattgatatc aaggcaccag
     3301 attgatagcc tttttttgtt ttattaagca ggtattttac tgctccacac ccgagttggt
     3361 gcgtatattc aacgtcaaat ggtattggtt ttgcacatct cagttcatac cctattgtaa
     3421 catca
//
LOCUS       DAORYU010000156         3384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_18692_length_3384_cov_4.212977, whole genome shotgun sequence.
ACCESSION   DAORYU010000156 DAORYU010000000
VERSION     DAORYU010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18692_length_3384_cov_4.212977"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="PLW07_05460"
     CDS             complement(<1..421)
                     /locus_tag="PLW07_05460"
                     /inference="COORDINATES: protein motif:HMM:NF016827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50007.1"
                     /translation="MDEQRLQQRLSQVQVNIIYQRILQSTALDLVQMCEDIVGENPML
                     EFEKIYSESSAYTQYRYQSIAAKETLADNLKKQIAYLNTEPIISKTAIFIVDLIDRDG
                     YLRFTVEEICNITGASLSTVEKALTVVQSLEPPGIGAR"
     gene            500..1174
                     /locus_tag="PLW07_05465"
     CDS             500..1174
                     /locus_tag="PLW07_05465"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HOL50008.1"
                     /translation="MTEWDWLLRIFITGILSGIIGWERERHARAAGFRTLILVGMGCC
                     LAMVVSLRISELFLSAESPFLRVDPARIAYGVLTGIGFIGAGTIIRDMGKVRGITTAS
                     CLWVVSAMGLAVGCGYYFLGIGTTILVLITLLTLKGVESRIPHDAYNRIKIRFSGETS
                     NIIDQILSILTKKGYQILEYSILFQKQENISAVEVQCRSRGKRNIISVLEDLKSITEV
                     LEVECK"
     gene            complement(1176..2555)
                     /locus_tag="PLW07_05470"
     CDS             complement(1176..2555)
                     /locus_tag="PLW07_05470"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HOL50009.1"
                     /translation="MVKMGFGCRKVTPPVGSGLLGYFQTRISKGVNDDLYARSICFDG
                     GEKPVFVVSADFCWVEASVVEKVKMLISERLGIEEPDVAIHGTHTHTGPITGWNKQLI
                     FGENIPVDEKYIEQLPVMICNAIEDSYKFRKDVLIGFDCTEVEGISFIRRYRMKNGTV
                     VTNPVDRSKIVEPSGDIDQTLNVMKVTDVNNKLIGIVLNFALHPDTVGGDFISGDWPG
                     LLVERCSKHFGCDTLFFNGAAGDINHINPYDSKTRSPEITEKIVQTLFTNIKDIVQKI
                     ECLPAEKLACSRDVFKMPKIKINEQGIEQAREWIQKYPSTNLRAIVGRAILKRAEISS
                     DFVNNDVLLLSVDRKMCAFFMAGELFSSIGLKIKDMMDFDFKWVVENCNGVVGYIPDE
                     RAFKAAEENKKIKGEEFPDVDVCESIGMEASYETSPISCRLAPDVEKAILSQFQQLLE
                     RHNKLIARQ"
     gene            complement(2548..>3384)
                     /locus_tag="PLW07_05475"
     CDS             complement(2548..>3384)
                     /locus_tag="PLW07_05475"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: protein motif:HMM:NF016438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOL50010.1"
                     /translation="LPLVADIHYDWKLAVDAMESGADKIRINPSNTQKNGVVKVIKEA
                     KKKRIPIRLGLNSGSIKITGRGLVKDIVCAASDAVKLCQDHDFFDIVISLKTPDVVAT
                     IESYRAIDEMFDYPLHLGVTEAGWGDAGLVKNALGIGVLLSEGIGNTIRVSLTDSPDK
                     EVKAGQAILQALKIRKFFPEIISCPTCGRCQVPLKKILLTFSNRIIKMVKMYPELRNF
                     TIAIMGCSVNGPGEAKQADIGIAGGKDRFAMFARGSIIGTYFTEKIEEEFFNKLKEML
                     HG"
BASE COUNT          990 a          744 c          574 g         1076 t
ORIGIN      
        1 cgcgtgctcc aatacctggt ggttcaagtg attgaacaac agtaagcgct ttttccacag
       61 tagacaaact ggcacctgta atattacaga tttcttccac agtgaaacgg agataaccat
      121 ccctatcaat gagatcaaca atgaatattg cggtttttga aattattggt tcagtattaa
      181 gatatgcaat ctgttttttt agattgtccg caagagtttc ctttgctgca atggattgat
      241 acctgtattg tgtatatgcc gatgattccg agtaaatttt ttcaaattca agcatgggat
      301 tttctcctac aatatcctcg cacatctgaa caagatcaag cgccgtggat tggagaatcc
      361 tctgataaat aatatttacc tgaacctggg acaatctttg ttgcaacctt tgttcatcca
      421 tgcttatatt ttagaaaaaa aatataattt tacaaactat tctttttgac atttcagaaa
      481 aaaataatag aatacagata tgaccgaatg ggattggtta ctcagaatat ttattactgg
      541 aatccttagc gggatcatag gatgggaaag ggaaagacat gcccgtgctg caggatttcg
      601 tactctcata ctggttggca tgggttgttg tcttgcaatg gtagtatcat tacgaatatc
      661 cgaacttttt ctttctgccg aatctccatt tttgagagtt gaccctgcaa gaattgcata
      721 tggtgtcctt acaggcattg gatttatcgg tgccgggact attatcagag acatgggaaa
      781 ggtcagaggt attaccactg cgtcgtgtct gtgggttgta agtgccatgg gacttgctgt
      841 gggatgcggt tattatttcc tcggcatcgg aacgacaatt ctcgtcttga taaccctgct
      901 caccctcaaa ggcgtggaat ccagaattcc ccatgatgct tataacagaa ttaaaataag
      961 attttctggg gaaacttcaa acatcattga tcagatatta tcaatactca caaaaaaggg
     1021 ctatcaaatt cttgaatatt ccattttgtt tcaaaaacaa gaaaacattt cagctgtgga
     1081 agttcagtgc agatcaagag gtaaaagaaa tatcatcagc gtacttgaag acctgaaatc
     1141 aattactgaa gtccttgagg ttgaatgcaa gtaaattact gccgtgcaat cagtttgtta
     1201 tgtctttcca gtaattgttg aaattgcgaa agaattgcct tttctacatc aggagccaac
     1261 ctgcatgaaa tcgggctggt ttcgtaactt gcttccatac caatggactc acacacatca
     1321 acatccggaa attcttcacc ttttattttt ttattttctt ctgctgcctt aaaagctctt
     1381 tcatcaggaa tatatcccac aaccccattg cagttttcaa caacccactt aaaatcaaaa
     1441 tccatcatat ccttaatctt caaaccaatg cttgaaaaaa gctctcctgc cataaaaaac
     1501 gcacacattt ttctatcaac agataaaagt aaaacatcgt tattcacgaa atcagaactt
     1561 atttctgctc ttttgagaat agccctgcca acaattgctc taagattggt tgagggatac
     1621 ttctgaatcc attctcttgc ctgttcaatt ccctgctcgt ttatttttat tttcggcatc
     1681 ttgaaaacat cgcgggaaca tgcaagtttt tcagcaggca ggcactcaat cttctggaca
     1741 atatctttta tgtttgtaaa aagcgtctgg acaatctttt ctgtaatctc tggactgcgg
     1801 gttttactat catagggatt gatgtggtta atgtcccctg ctgctccatt gaaaaataat
     1861 gtatcacaac caaagtgttt tgaacatctt tcaacaagaa ggcctggcca gtcccctgaa
     1921 ataaaatctc cgccgactgt atcagggtgc aatgcgaaat tgagaacaat ccctatcagt
     1981 ttgttattaa catctgtcac cttcataacg ttcaatgttt gatcaatatc cccggaaggt
     2041 tcaacaattt tggacctatc aacaggattt gtaacaacag taccattttt cattctgtat
     2101 cttctaataa aggaaatccc ttccacctcc gtgcaatcaa aaccaataag aacatctttc
     2161 cgaaatttgt acgaatcttc aatcgcattg catatcatta ctggcaattg ttcaatatat
     2221 ttttcatcaa caggtatgtt ctctccgaaa ataagttgct tgttccaacc tgtaataggc
     2281 cctgtgtgcg tatgtgtccc atgaattgca acatctggtt cttctattcc aagacgttct
     2341 gaaattaaca ttttaacttt ttcaacaaca gatgcttcca cccagcaaaa atctgcagat
     2401 actacaaaaa ctggtttttc tcctccatca aaacagatac tccttgcata aaggtcatca
     2461 ttaacacctt tcgaaatcct tgtttgaaaa tatccaagca gaccgcttcc gaccggtgga
     2521 gtcacctttc tacacccaaa tcccatttta accatggagc atctccttta atttattgaa
     2581 aaattcttct tcaatttttt ctgtaaaata tgtaccaatt atactacctc tggcaaacat
     2641 tgcaaatcta tccttgccgc cagcaatacc aatgtctgcc tgctttgcct ctccaggacc
     2701 attaacactg catcccataa tcgcgattgt gaaatttctc aattccggat acatttttac
     2761 catttttata atcctgttgc taaatgttaa aagaattttt ttaagaggca cctgacacct
     2821 gccacatgta ggacatgata tgatttccgg gaaaaatttt cgtatcttga gtgcctgtaa
     2881 aatcgcctga cctgccttca cttctttgtc tggcgagtcc gtcaaggaaa ctctgattgt
     2941 attgccaatg ccctctgaca gaagaacacc tatgccgagt gcgtttttca ccagtcctgc
     3001 atcaccccag cccgcctcag taactccaag atgtaaaggg taatcaaaca tctcgtcaat
     3061 agcgcgatag gattctatcg tcgcaacaac atcaggggtt tttaaggaaa tcacaatatc
     3121 aaaaaaatca tgatcctgac acagtttaac ggcatcggat gccgcacaaa caatatcctt
     3181 caccagtccc cttcctgtaa ttttaataga tcctgagttc aatccaagtc taatgggaat
     3241 tctttttttc tttgcttctt ttataacttt aaccactcca ttcttctgtg tatttgacgg
     3301 attgattctt attttgtctg cacctgactc catggcatca acggcaagtt tccagtcgta
     3361 gtgaatatct gctactaatg gcaa
//
LOCUS       DAORYU010000157         3337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_19026_length_3337_cov_7.165753, whole genome shotgun sequence.
ACCESSION   DAORYU010000157 DAORYU010000000
VERSION     DAORYU010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19026_length_3337_cov_7.165753"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..1781)
                     /locus_tag="PLW07_05480"
     CDS             complement(123..1781)
                     /locus_tag="PLW07_05480"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOL50011.1"
                     /translation="MKRVNPVKKDDGLYFADMSKCYPSSAISEKRSRKTWKKFHYETE
                     KMSGILLVACYGVDPPVLSLPVKVKGRYKVFVGLYGEHGFYAGIRIKLKKDYCFHHLV
                     SWNSLSKGAVINEVFWKEAEMIDEVIEIAPYSKKDDDRITSGIAYIRLVPAEEKTEPD
                     RKPDIYLAAFNDAYSFIYYHRPVTKKEIQEQIEVFRDTPFKKIYWGMAAGDVATYPTN
                     IGSRTNIDLDVYPRIGDRNYAESCRILRNKKFDILKIAVEHAKKIGLEFHVYHRLGFV
                     TGQPPWEEMMYSKFFYKNQKFLCYDRDGTKISRLSYAYPEVQQHCINLYKEVASYGID
                     GINFAFHRGVPLVLFEKPIVEGFKSRFGEDPRKLPDTDQRLFQFRSSIITGYLERVRK
                     EFPWLELAATVLINEANNRFYNLDIKNWIDKKIIDVLIPYGQDRGLAHKGVIDMEYFS
                     KLTKGTSCQLCPELRAVRSWLTPEQHYVDMASYFYKYDIDGICFWDIESRFVFPKEWR
                     IINELCWKKGLKSRINRIKDDFRDIPLKTLNGYAANRYPFWWSY"
     gene            complement(1778..>3337)
                     /locus_tag="PLW07_05485"
     CDS             complement(1778..>3337)
                     /locus_tag="PLW07_05485"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOL50012.1"
                     /translation="NACVRIIAPEPDTVVTIKGIEDKRAFGKLAEKEETSIELKDKKF
                     IEINYGNAWGFREIKSNKPIMVYVTPYSLNNFTMLHPTYESGKEKYTGKEFYGGINPY
                     PNNNFYPCIVVTCIDGSADVYLEEMKDDGTVVKKTQASKTLKQGEEYVFYLESGVPWY
                     HIVSTGDIYVYTSRAMLKGYAYTRDTLFLPDVNGVFCGTDFIANIGMETLVYSSKPDC
                     ELTVYSFDGKFAPFKMQLEKGYSLLGCPRVYGEKQYLISFSSPGLVQFNSGYSMSYAM
                     PVDGPVASAYFGSEMVKKVMPPAQSNPVVARKVANEYAGLSVEQKIARFKNEYLMKKK
                     PVKIVAFGDSVTQSARVSGAETFYQIFAKQLREKYGYDNITSVNRGIGGNNVFHALSR
                     IREDVVAENPQLVFVMFGVNDGYRGMNMDVYEKNMRKIVEILENETDALIILLGPTPL
                     TTSKDAFRDYDKTVEKLSKEKKIPCLSMHEVLMKEPEWDKNLYTDFCHPGPQGHKTMA
                     EGIWRFFEGEK"
BASE COUNT          929 a          764 c          531 g         1113 t
ORIGIN      
        1 gcaacatatg tattgattgt tttgtcctgc atattttgtt gctggcataa cagctgaata
       61 gcagcaatga aaaggcacat accagcaaag ttaattttcc tcattttgca ctccttctcg
      121 aattaatatg accaccagaa tggatatcta tttgctgcat acccattaag ggttttcaag
      181 ggaatatctc tgaaatcgtc ttttatcctg ttgattctgc ttttcaatcc ttttttccaa
      241 cacaactcat taataattct ccattccttc ggaaatacaa atcttgattc tatgtcccaa
      301 aaacagattc catcaatgtc atacttatag aagtaactcg ccatgtcaac gtaatgttgc
      361 tctggtgtca gccaggacct tactgcgcgc aattcaggac acaactggca ggaagtgcct
      421 tttgtaagtt tagaaaaata ctccatgtca atcacgcctt tgtgggcaag ccccctgtcc
      481 tgtccataag gaatcagaac atctattatt tttttgtcaa tccaattttt tatgtcaaga
      541 ttataaaatc tgttgtttgc ttcgtttatt aaaacagtcg cagcaagttc aagccaggga
      601 aattcctttc tcactcgttc caaataccct gtaatgatac tgcttctgaa ttgaaaaaga
      661 cgctggtctg tatcaggtag ttttctcggg tcttctccaa atctgctctt gaaaccttcc
      721 acaatgggtt tttcaaacag aacaagcggc acacctctgt gaaaggcaaa atttatccca
      781 tcaattccat aagaagcaac ttccttgtac aggttgatac agtgttgctg cacttcagga
      841 tacgcataac tcaaccttga aatttttgtt ccatctctgt catagcataa aaacttctga
      901 ttcttgtaaa aaaactttga atacatcatt tcttcccatg gcggctgtcc tgttacaaac
      961 cccaatctat gatagacatg aaattcaaga cctatcttct ttgcatgttc cacagcaatc
     1021 ttcaaaatgt caaacttctt atttctcaaa attctgcaac tttccgcata attcctgtcc
     1081 ccaatccttg gatatacatc aaggtcaatg tttgttcttg agccaatatt ggtgggatat
     1141 gtggcaacat cccctgcagc cattccccag tagatttttt taaaaggtgt atcgcgaaaa
     1201 acttctatct gttcctgaat ttcttttttt gttactggtc tgtgataata gatgaaagaa
     1261 taagcatcat tgaatgcagc caggtatata tcgggttttc tgtctggttc agttttttct
     1321 tcagcaggca caagcctgat gtatgcaata ccagaagtaa tgcggtcatc atcttttttg
     1381 ctataaggtg caatttcaat cacttcgtca atcatttctg cttctttcca gaacacctca
     1441 ttaataacag ctccctttga aagggaattc cacgaaacaa gatgatgaaa gcaataatct
     1501 ttcttcaatt tgatccgtat accggcataa aatccatgtt ctccgtaaag tcccacgaaa
     1561 accttatatc tgcctttgac tttcacaggc aaactcaata ctggtgggtc cacaccataa
     1621 caggcaacaa gtaaaatgcc tgacattttt tctgtttcat agtgaaattt tttccaggtt
     1681 tttctgctcc ttttttcgga aatcgctgaa gaaggatagc atttgctcat gtccgcaaaa
     1741 taaagaccat cgtctttctt tactggattg acccgcttca tttttcacct tcaaaaaatc
     1801 tccagattcc ttctgccatc gttttatgtc cctgtggtcc tggatggcag aagtcagtat
     1861 aaagattctt atcccattct ggttctttca tcaaaacctc atgcatgctc aggcagggga
     1921 tttttttctc tttgctcaat ttttccacag ttttatcata atccctgaag gcatccttgc
     1981 tggtggtcaa aggagttggc cctaatagta taatcagggc atctgtctca ttttccagaa
     2041 tttcaacaat ttttctcata ttcttttcat aaacatccat gttcattccc ctgtatccgt
     2101 catttactcc aaacatcaca aaaacaagct gagggttttc agcaaccaca tcttccctaa
     2161 ttctgctcaa tgcatggaaa acattgttac caccaatgcc cctgtttact gaagtgatgt
     2221 tgtcataacc gtatttttct ctcaactgtt ttgcaaatat ctgataaaaa gtttcagcac
     2281 cacttaccct tgcactttgg gtcacagagt caccaaacgc aacaattttt accggctttt
     2341 tcttcatcag atactcattt ttaaatcttg ctattttttg ctcaactgaa agtcctgcgt
     2401 attcgttagc cacttttctc gcaaccacag gattgctctg cgcaggaggc atcacctttt
     2461 tcaccatctc agaaccaaaa tatgctgatg ccacagggcc atcaacaggc atcgcataac
     2521 tcatggaata ccctgaatta aactggacaa gacccggact ggaaaatgaa ataagatatt
     2581 gtttttcgcc gtaaaccctc ggacatccaa gaagtgaata acctttttcc agttgcattt
     2641 tgaaaggagc gaatttcccg tcaaaactat aaacggtcag ttcacaatct ggctttgaag
     2701 aatatacgag agtttccata ccgatgtttg caataaaatc tgttccgcag aacactccat
     2761 tgacatcagg caaaaacaga gtatcccttg tatatgcata cccttttagc atcgctcttg
     2821 atgtatagac atatatatca cctgttgaaa caatgtgata ccacggaacc cctgactcaa
     2881 gataaaaaac atactcttcg ccctgtttca gggtttttga cgcctgcgtt tttttaacaa
     2941 ccgtaccatc gtctttcatt tcttccagat aaacatctgc gctcccatca atgcatgtga
     3001 ccacaatgca gggatagaaa ttattgtttg gatatgggtt tattcctcca tagaattctt
     3061 ttccagtgta tttttctttt ccagattcat aggttggatg taacattgta aaattgttaa
     3121 gagaatatgg cgtaacataa accataattg gcttgttgga ttttatttct ctgaatcccc
     3181 acgcgtttcc ataattgatt tctataaatt tcttatcttt caattcaatg cttgtttcct
     3241 ctttttctgc cagttttcca aatgctcttt tgtcttctat tcccttgatt gttacaacag
     3301 tgtctggttc aggagcaata atcctcacac acgcatt
//
LOCUS       DAORYU010000158         3320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_19142_length_3320_cov_3.998162, whole genome shotgun sequence.
ACCESSION   DAORYU010000158 DAORYU010000000
VERSION     DAORYU010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19142_length_3320_cov_3.998162"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PLW07_05490"
     CDS             <1..234
                     /locus_tag="PLW07_05490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOL50013.1"
                     /translation="ISNRDADEISQMRRQTLQRALEHLTVRQRTVFNLRIYEELSYEE
                     IAKIMKIKVGTAKATFFQSVEKIKTIMKEMEKK"
     gene            231..722
                     /locus_tag="PLW07_05495"
     CDS             231..722
                     /locus_tag="PLW07_05495"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOL50014.1"
                     /translation="MNCEQIKKKLVDYADGSVSKPEQNQIQSHLAECPACRKEFETLT
                     EIVVTARSIKVPEHDENFWNVKYQQVIDRAEHRQHRTVFIKKLRLGFGVVGILLVLFG
                     SRVFIGKNAPVRFPEVSEVAYNVPEDVLSENKLPFSIDELNKVVDLLGPEDHMMILSE
                     YLR"
     gene            750..1343
                     /locus_tag="PLW07_05500"
     CDS             750..1343
                     /locus_tag="PLW07_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010479675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOL50015.1"
                     /translation="MEKKIINLPSPLLKTSVSVEETFYRRHSTRSFRKIPLTLNEVSQ
                     LLWAAYGKNAYGKKTVPSAGAAYPFDIYLVAGQVEKIEAGLYLYEAERHVLIPVKQGD
                     LRERLAIASHNQEFIAHAPATIVLVAQFTRTTALYGTRGERYVLLDAGHIGQNVSLQV
                     ESIGLGTCMIGAFDDEQVLEVLGVTGNIVYMMPVGKP"
     gene            1340..3055
                     /gene="polX"
                     /locus_tag="PLW07_05505"
     CDS             1340..3055
                     /gene="polX"
                     /locus_tag="PLW07_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HOL50016.1"
                     /translation="MRNNEIADVLDSIADVLEFEGENVFKINAYRKVARKIREIPEDI
                     EQLYQAGNLSKIPGIGKGISEHIQEYLKTGSIAKYKEILSKVPSTFVEMMKIPNLGPK
                     TLKLLYEHFKIQTVEDLKNVLETQEVQNLPGMGEKKIENIKKGIQLYLTKRIHQRISL
                     GIALPVVDEIVRYMQPVCENISPCGSLRRMKETIGDIDILCTADNKEKVVKKFVSYPG
                     VQSIIAEGETKSSIIVKDHMLQIDLRVVEPDSYGAALQYFTGSKEHNIKLRTIAKEKG
                     LKISEYGVFRDDEKIVGRSEEEIYKMFGMPLIPPELREDRGEIEAAIAGKLPDLVEHE
                     NIKGDFHIHTNYSDGVSTIEEIVKKAINMGYQFIGLCDHSQTSKFAGGLTVERLRERN
                     REIDNIASKYPQIKILKGAEVDILGDGSMDYPDEILAELDFVIASIHQGFKKNVTERM
                     IKAMENRYVDIIGHPTGRLISSREGYDVDIDAVIEYAGKTGTALEINAYYDRLDLNDI
                     NVMKAKEHNVMVAIGTDAHNIKMMEYMKLGIAVSRRAWLENNNILNCQKLDNMFIKRK
                     KTKTE"
BASE COUNT         1144 a          534 c          755 g          887 t
ORIGIN      
        1 atcagtaaca gggatgctga tgaaataagc cagatgagaa ggcaaactct tcaacgggcg
       61 cttgagcatc tcactgtgag acagcgtaca gtgtttaatt tgaggatata tgaagaactt
      121 tcctatgagg aaattgcgaa aattatgaaa ataaaggtgg gaacagccaa agcgaccttc
      181 tttcaatcag ttgaaaaaat aaagacaatt atgaaggaga tggagaaaaa atgaattgtg
      241 aacaaataaa aaagaaactt gttgattatg ctgatggcag tgtcagtaag ccagaacaga
      301 atcagattca gtcccatctt gcagaatgtc cggcgtgcag gaaagaattt gaaactttga
      361 cagagattgt ggttactgcc agaagtataa aagttcctga gcacgatgaa aatttctgga
      421 atgtgaagta tcaacaggtt attgataggg cagaacatcg acaacacagg actgttttca
      481 tcaaaaagct caggttgggt tttggggtgg tagggatact gctggtattg ttcggatcaa
      541 gggttttcat tggtaaaaat gctcctgttc ggtttcctga agtgtctgag gtggcttaca
      601 acgtgcctga ggatgtgctt tcagaaaaca aactgccttt ttcaattgac gaattgaata
      661 aggtggttga cttgctcggt ccagaagacc acatgatgat tctttctgaa tacttgcgtt
      721 gacatcacaa tcaagtatac aatcattata tggaaaagaa aattatcaat cttccatctc
      781 ccttgttgaa aaccagtgtc tctgttgaag agacatttta cagaagacat tcaaccaggt
      841 ccttcagaaa aattccgttg actctcaacg aggtctctca acttctctgg gctgcatacg
      901 gaaaaaacgc atatggtaag aaaactgttc cctcagcagg cgcagcatat ccttttgata
      961 tctatcttgt ggctggacag gtggaaaaga ttgaagccgg gttgtatctt tatgaggcag
     1021 aaaggcacgt gttgataccc gtaaaacagg gtgatttgag agaacgactt gctattgcgt
     1081 cacataacca ggaatttata gcgcatgcgc ctgctacgat tgttcttgtg gctcaattta
     1141 cccgtaccac tgcactttac ggaacaaggg gcgaaagata tgtgttgctt gatgcaggac
     1201 acattggaca gaatgtttcg cttcaggttg aaagtatcgg gctggggaca tgtatgattg
     1261 gtgcatttga tgatgagcag gtcctggaag tactgggcgt tactggcaat attgtgtata
     1321 tgatgccagt gggcaaacca tgagaaataa tgaaattgct gatgtcctcg acagtattgc
     1381 tgatgtcctt gaatttgaag gagaaaatgt ttttaaaatc aatgcctacc gaaaagtagc
     1441 aagaaaaatc agagaaattc cagaagacat tgaacaattg tatcaagccg gcaatttatc
     1501 aaaaattcct ggcataggaa aaggtatttc agagcatata caggaatacc ttaaaaccgg
     1561 ctcaattgca aaatataaag aaatcctctc caaggtgcca tcaacatttg ttgaaatgat
     1621 gaaaattcca aatctcggtc caaaaaccct taaacttctt tatgaacact ttaagattca
     1681 aacggttgaa gatttgaaga atgttctgga aacacaggaa gttcaaaatt tgccgggtat
     1741 gggcgaaaaa aagatagaaa acataaaaaa gggaatacaa ttatatctaa caaaaaggat
     1801 ccatcaaaga atttctcttg gtattgcact accggttgtt gatgagattg tcagatatat
     1861 gcaacctgtt tgcgaaaata tttctccatg cggttcattg agaagaatga aagaaaccat
     1921 tggtgatatt gatatcctgt gtacagcaga caacaaagaa aaagtggtga aaaaatttgt
     1981 cagttaccct ggggttcaat caatcattgc tgaaggagag acaaaatcat caattattgt
     2041 aaaagaccat atgcttcaaa tagaccttcg ggttgttgaa ccagattctt atggcgctgc
     2101 gttgcagtat tttacaggtt caaaggaaca taatattaaa ttgcgcacga ttgcaaagga
     2161 aaagggattg aaaatcagtg aatatggggt ttttagagat gacgagaaga ttgtgggcag
     2221 gtcagaagaa gaaatatata aaatgttcgg gatgcccctt attccaccag aattaaggga
     2281 agacagggga gagatagaag cagccattgc cggcaaactg ccagaccttg tcgaacacga
     2341 aaatattaaa ggagattttc atatccacac aaattattct gatggagtca gtaccattga
     2401 agaaatcgtg aaaaaagcaa tcaacatggg gtatcaattc attggactgt gtgatcattc
     2461 tcaaacaagc aagtttgctg gcggccttac tgttgaaaga ttaagggaaa gaaatagaga
     2521 aattgacaat attgccagca aatacccgca gataaaaatt ttgaaaggcg ctgaagttga
     2581 tatacttggt gatggaagta tggattatcc agacgaaatc cttgctgaac tggactttgt
     2641 tattgcttca attcatcagg gatttaagaa aaatgtgact gaacgaatga taaaagcaat
     2701 ggagaataga tatgttgaca ttatcggaca tcccacaggt agattgatat caagcaggga
     2761 aggttatgat gttgacatag atgcagtgat tgaatacgca ggtaaaacag gcactgcact
     2821 tgaaatcaat gcctactatg atcggcttga cctcaatgac atcaatgtga tgaaggcaaa
     2881 agagcataat gtgatggtcg caattggaac agatgctcat aatataaaaa tgatggaata
     2941 tatgaaactc gggattgctg tttcaagaag ggcatggctt gaaaataaca atatcttgaa
     3001 ctgccagaaa cttgataata tgtttatcaa aaggaaaaag acaaaaactg agtaaatatc
     3061 cgttttatga tttcttgcag tatggtaaaa taacctgtta cttaattctt caatcaggtg
     3121 aatctatgtc taaagaaaca ttaacgatac tgatacaccc tggacgactc gtatttgtca
     3181 tagaaaaaat tgccgaaaaa aataataatt caggaacttc tggaggttat catggttgca
     3241 aaggtgctta cggttgaagg gaaatcaatt gctgaagtat gggaaaaaag tgtgatggtg
     3301 ctatgggaaa aaggcgctca
//
LOCUS       DAORYU010000159         3307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_19230_length_3307_cov_4.018758, whole genome shotgun sequence.
ACCESSION   DAORYU010000159 DAORYU010000000
VERSION     DAORYU010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19230_length_3307_cov_4.018758"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="PLW07_05510"
     CDS             complement(<1..373)
                     /locus_tag="PLW07_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOL50017.1"
                     /translation="MPPYLFSLDAPYGKIILAVYNYYTTKPGEIMPLQPISPEQARAI
                     CNLESLEFETTKDIPPIEGIIGQERAARALNFGLQMEQDGYNIYVSGIPGSGRTTSVE
                     SAVKEIAKTKPTPDDWCYVYNF"
     gene            352..1392
                     /locus_tag="PLW07_05515"
     CDS             352..1392
                     /locus_tag="PLW07_05515"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOL50018.1"
                     /translation="MKKGMAAYLLKKKRFVIDEFEIPDCKDDEVLIRIRAIGVCGSDA
                     HYFLDGRIGDQIVPEKFIIGHEASGEIVATGKHVKSVKEGDRVVVEPGISCGKCEFCI
                     TGRPNLCPYVKFLGTPPVYGAFRQYIVMPEKNVIKLPDSLSFAEGTLAEPLAIALYGV
                     RLANFLAGDSIAILGAGPIGLSVLFCCRTGGARQIFVTEIIEQRAEMAKKLGADEIYL
                     ANRQDIVKAINEKTNNRGVDIAFECAGEQQTIKQMIDIAAIGGKAVIFGIPAEDEVCF
                     DPHLSRRKQLPIFAVRRSAFTTEAALALMCKSHIKFSSIITHKFPLDEIQNALEIVST
                     RSDGVIKAIIEP"
     gene            1405..2049
                     /locus_tag="PLW07_05520"
     CDS             1405..2049
                     /locus_tag="PLW07_05520"
                     /inference="COORDINATES: protein motif:HMM:NF003994.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOL50019.1"
                     /translation="MAKKKQVRIPQETIRRIALYLRSLRKLKELKFNIISSGQITAYL
                     NVSPEQFRKDLSYFGPVGKPGVGYRVDNLIHHLERILGIDRECRIILVGVGKLGSALL
                     AYPGFLNFNFRIVGAFDSDKEKIGKTLGKVRVYDVSEMKKFIQHDRVQLAILAVPVES
                     AQHVAEKLVACGIKGILNFAPVNLNLTQPVRVLNVDMATELMTLTYFVNRILAG"
     gene            2233..>3307
                     /locus_tag="PLW07_05525"
     CDS             2233..>3307
                     /locus_tag="PLW07_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50020.1"
                     /translation="MVIVVNCVFAQTAGVIYSQNSSNNSSNIIFYDTSSGETTLLTAF
                     AETDNGRALTISAYGAGGLIYFTRSSPANGRPNPTIWRINIDGSGLTDFLSPDTEISY
                     TGVAVSPDGSTIAYTANDKDNPSVYHLYVCNSDGSNRRRLTFDPTWNCSYPSFIDNST
                     ILVKMQNGSLENYYSVTTTGEITRLTNNETLSPYFPRLGRPMLNNSRNAIVYAKQVQD
                     VSGYRKWAIYQLQPLDGTGTENLITDFLYFTESDPLLQEEPYPAYGETDSTMFLCGSL
                     SGNVYSLYTVNIPVINPYVEQLTSGALHTTLPVFIMISAKLQRYVYSQDGYVYVRNEN
                     GNSTRITAGSGNRHPTINRRGTII"
BASE COUNT          994 a          607 c          715 g          991 t
ORIGIN      
        1 aaaaattata cacataacac cagtcatcag gtgttggttt tgtttttgca atttctttca
       61 ctgctgattc cactgaagtt gttcttccgc ttccaggtat tcctgataca tagatattat
      121 agccatcctg ttccatttgt agtccaaaat taagcgctct tgcggcacgc tcctgtccga
      181 tgatgccttc aattggtggt atatcttttg tcgtctcaaa ttcaagcgat tccaaattac
      241 agattgctct cgcctgttct ggtgaaatcg gttgcagtgg cattatttct cctggttttg
      301 tggtataata attataaacg gctaaaatta ttttaccata cggagcgtcc aatgaaaaaa
      361 ggtatggcgg catatcttct caaaaagaaa agatttgtga ttgatgaatt tgaaatacct
      421 gattgcaagg acgatgaggt cttaattcgt atcagggcaa tcggtgtgtg cggttcagac
      481 gcgcattatt ttcttgatgg aaggattggg gatcaaattg tgccagagaa attcattatt
      541 ggtcatgagg cgtctggcga gattgttgcc acagggaaac atgtgaaatc cgttaaagaa
      601 ggagacaggg tagtggtaga acccggtatt tcctgtggaa aatgtgaatt ttgcattact
      661 ggcagaccta atttatgtcc gtatgttaaa ttccttggaa caccacctgt ttatggcgca
      721 ttcaggcagt acatcgtgat gcctgaaaaa aatgtgataa aactaccgga ttctctttcc
      781 tttgcggaag gcactcttgc tgaaccactg gcgatagcgc tctacggagt acgtcttgca
      841 aattttttag caggagatag tattgccatt cttggcgcag ggcccatagg actatcagtt
      901 cttttttgtt gcagaaccgg tggcgcaagg cagatttttg tgacagagat tatagaacaa
      961 cgcgcagaaa tggctaaaaa acttggcgca gacgagatat atcttgcaaa taggcaagat
     1021 attgtcaaag caatcaacga aaaaacaaat aacaggggtg tggacattgc gtttgagtgc
     1081 gcaggagagc aacaaacaat aaaacagatg attgatattg ctgcgattgg tggaaaagct
     1141 gtcatatttg gaatccccgc agaagacgaa gtttgttttg acccgcacct gtccagaaga
     1201 aaacaacttc caatttttgc tgtgagaagg tctgctttta caacagaggc agcacttgcc
     1261 cttatgtgta aaagccacat caagttttct tctattatca ctcataaatt tccacttgat
     1321 gaaattcaaa atgcattaga aattgtttct acaagaagcg acggtgtaat caaagcaatt
     1381 attgaaccat aaccgtcatg taatatggcg aaaaaaaaac aggtaagaat accgcaggaa
     1441 actatcagaa gaatagcgtt atatttaaga agtttgagaa agctcaaaga gttgaaattc
     1501 aatatcatat cgtcggggca gattactgcg tatctcaatg ttagtcctga acaatttcgc
     1561 aaggaccttt cttatttcgg tccagtaggc aaacccggtg tcggttacag ggttgacaac
     1621 ctgatccatc atcttgaaag aatactgggt attgacaggg aatgcaggat tattcttgtt
     1681 ggtgttggta aacttggttc tgcgctgctt gcgtatcctg gatttttaaa ttttaatttc
     1741 aggattgttg gagcatttga cagtgataaa gaaaaaattg gtaaaaccct tggaaaggtt
     1801 agagtatatg atgtttctga aatgaaaaaa tttattcagc atgaccgcgt gcaactcgca
     1861 attcttgctg ttcctgttga atcggctcaa catgttgcag aaaaacttgt tgcctgcgga
     1921 ataaagggca ttctgaattt tgctcctgtg aatttgaatc ttactcagcc agtacgggtt
     1981 ctcaatgttg atatggcgac tgaactgatg accctgacat atttcgtaaa tagaattctt
     2041 gcgggatagg gattattgaa attatggtct gctgcgaaaa aaatatttgt gatgttcgtt
     2101 ttctgaagat agaaattgtt tttgacaggt acgactatta cgatgtaaaa tgattcaaag
     2161 atgcgtaatg tataacaggg ccaataaatg aataagaacc gattgttcgg gtggttttgt
     2221 gttgcaggcc tgatggtgat tgttgtaaat tgtgtttttg ctcaaacggc tggggtgata
     2281 tactcacaga attcttccaa taatagttcc aacattattt tttatgatac ttcctctggt
     2341 gaaacgactc tccttacagc atttgcagaa acagacaatg gcagggcatt gacaatttcc
     2401 gcttatggtg cagggggatt aatatatttt accagaagtt ctccagcaaa tgggagacca
     2461 aatcccacga tatggagaat caacattgat ggtagcggat taactgattt tctttcacct
     2521 gatacagaaa tatcatatac tggtgttgct gtttctccag acggaagcac cattgcatat
     2581 actgccaatg acaaagataa tccatctgtc tatcaccttt atgtctgcaa tagtgacggt
     2641 tcaaacagaa gaagattgac ctttgaccca acctggaatt gttcctatcc ttctttcata
     2701 gacaacagta ctattcttgt taaaatgcaa aatggcagtc ttgaaaacta ttattcagta
     2761 acgaccacag gtgaaataac ccgtcttacc aacaatgaaa ccctgtctcc atactttcca
     2821 cgacttggca gacccatgtt gaataatagc agaaatgcga tcgtttacgc aaaacaggtg
     2881 caggatgttt ctggatacag aaaatgggca atttatcagt tgcaaccact tgatggtacc
     2941 ggtacagaaa atctaatcac tgattttctt tattttactg aatctgaccc attgcttcag
     3001 gaagaaccat atcccgccta tggagagaca gacagtacaa tgtttttgtg cggatctctg
     3061 tcgggcaatg tgtacagttt atacactgta aacattcctg ttatcaatcc atatgtggaa
     3121 caattaactt ctggtgcttt gcatacgaca ctaccagttt ttattatgat atcagcgaaa
     3181 ttgcaacgat atgtgtattc gcaggacgga tacgtgtatg tgagaaatga aaacggaaat
     3241 tcgacccgta tcactgcagg aagtggaaac cggcatccaa caatcaatag aaggggtaca
     3301 ataattg
//
LOCUS       DAORYU010000160         3300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_19288_length_3300_cov_5.750077, whole genome shotgun sequence.
ACCESSION   DAORYU010000160 DAORYU010000000
VERSION     DAORYU010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19288_length_3300_cov_5.750077"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="PLW07_05530"
     CDS             complement(<1..561)
                     /locus_tag="PLW07_05530"
                     /inference="COORDINATES: protein motif:HMM:NF024739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOL50021.1"
                     /translation="MLLVASVCIAEENLVVISPHWEGVKIEIEKAFKKYYYNIKQKDI
                     RIEWLDQGGTSDDLKYVESLFKKNPQTTGIDVFFGGGLDPYLRLKEKGYLASCKIPVK
                     ILKEIPKECNGIPNYDNKDYTWYGVVLASFGILCNKKALQFLGVSEPKKWSDLAKPEY
                     YSWVGTSDPRHSGSMHMMFEIILQANG"
     gene            complement(601..1446)
                     /gene="lipA"
                     /locus_tag="PLW07_05535"
     CDS             complement(601..1446)
                     /gene="lipA"
                     /locus_tag="PLW07_05535"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOL50022.1"
                     /translation="MNKFPDWVKKKIVVDEHYEETKAIIKQYSLHTVCTDARCPNIYE
                     CFSKKYATFMILGNVCTRACKFCSVNHNQKPQPVDPQEPEHIARAVQQLQLKYVVITS
                     VTRDDLDDGGAKHFADCIRAIRKYNPQTKIEPLIPDFKGNTAFLEVVLEAKPDILAHN
                     VETVPSLYSIVRPQADYSVSLKILEHAKKQGFLTKSAMMLGLGETEHQILQVMKDLRS
                     IDCDFFVIGQYLKPSPESLDVKEFVRPEVFEQYRVIGEEMGFKKVFAGVFCRSSYMAE
                     LALTE"
     gene            complement(1443..2762)
                     /gene="lpdA"
                     /locus_tag="PLW07_05540"
     CDS             complement(1443..2762)
                     /gene="lpdA"
                     /locus_tag="PLW07_05540"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOL50023.1"
                     /translation="MNEFDVIIIGGGPAGYPCAIRCAQYGLKTALIEKEKVGGCCLNT
                     GCIPTKTLFKIARTIQKPRIDGINAEISFDWQKILNHVKTNVVSRLNTGVGMLLRANG
                     VQYIQAEAKLCDNESVVANGNSLKAKNIVIATGARPNVPAIFCNDERIITTDTIWNLE
                     KLPGSLAIIGAGPIGCEFASILSCFGVKITIYEMMEQILPGKDKDIISVIEKQFAQKG
                     IQIKTGIKINSTDAISEEKILWATGRKPDATGAGEIGITLAAGGIVTDERMKTNIDNI
                     FAAGDVTGRWQLAYVATKEGEIAASNCAGKHEAISYENIPESIFTIPEIGCIGLSEKQ
                     ALEKQLNIKTGIFPYQALGKAHASGETVGLAKVIVDTDSHKLVGVHIAGEAATEIIHV
                     ASIAMSAGMTIEQMLRAYWCHPVFSEILMEALLVCEGHPIHIPRPKK"
BASE COUNT          876 a          745 c          607 g         1072 t
ORIGIN      
        1 gccatttgcc tgaaggataa tttcaaacat catatgcatg cttccactgt gacgcgggtc
       61 tgatgttcct acccagctgt agtactctgg ctttgcaagg tcgctccatt tttttggttc
      121 ggatacgccc aaaaactgaa gcgccttttt gttacacagt attccaaaac ttgctaacac
      181 cactccatac caggtgtaat ccttgttgtc ataatttgga atgccattac actctttcgg
      241 aatttctttc aaaattttta ctggaatttt gcaggatgca agataccctt tttcttttaa
      301 tcgtaagtaa gggtcaagcc cgccaccaaa aaaaacatca atgcctgtgg tctgtgggtt
      361 tttcttgaaa agggattcaa catattttaa gtcatcagaa gttccaccct ggtcaagcca
      421 ttctatcctg atatcctttt gcttaatgtt gtaatagtat tttttgaatg ctttttcaat
      481 ctctattttc acaccttccc agtgcgggct gatgacaaca aggttttctt cagcaatgca
      541 aactgatgca acaagcaaca taaaaaatgc aaaaaggtgt aaatgttttc tcattgtaat
      601 ttattcagtc agggcaagtt ctgccatata tgaacttctg caaaataccc ctgcaaaaac
      661 ttttttaaat cccatttctt cgccaatgac cctgtattgc tcaaaaacct ctggtcttac
      721 aaactctttc acatcaaggc tctccggtga tggtttcaaa tactggccaa taacaaaaaa
      781 atcgcaatca atactgcgca ggtctttcat cacctggagt atctggtgct ctgtttctcc
      841 aagacccagc atcatggctg attttgttaa gaaaccctgt ttttttgcat gttccagaat
      901 ttttaaggac acagaataat ctgcctgtgg tctcacgatg gaatacagag atgggactgt
      961 ttcaacatta tgagcaagaa tatctggctt tgcctcaaga acgacttcaa gaaaggcagt
     1021 gtttcctttg aagtctggaa ttaatggttc aatttttgtc tgtgggttgt actttctgat
     1081 tgccctgata caatcagcaa agtgttttgc tccaccatca tcaaggtcat cccttgtgac
     1141 agaagtaatc acaacatatt tcaattgcag ttgttgtact gcacgcgcaa tatgctctgg
     1201 ttcctgtggg tcaactggtt gcggcttttg attgtgattg acgctgcaga atttgcaagc
     1261 acgggtgcag acatttccga gaatcataaa tgttgcgtat tttttactga aacattcgta
     1321 aatattggga cacctggcat ctgtgcaaac agtatggagt gagtattgtt tgatgatggc
     1381 ttttgtctcc tcgtaatgtt catcaaccac aatctttttc tttacccaat caggaaactt
     1441 attcattttt ttggtctggg aatgtgaatg ggatgacctt cgcaaacaag aagtgcttcc
     1501 atcaagattt ctgaaaatac tggatgacac cagtatgccc tcagcatttg ttcaattgtc
     1561 atgccagcac tcatcgcaat ggatgcaaca tgaataatct ctgtggctgc ctcaccagcg
     1621 atatgaacgc cgacaagttt atgggaatca gtgtcaacaa tcacctttgc cagtcccaca
     1681 gtttctcctg atgcatgcgc cttgccaagc gcctgatatg gaaatatacc agtttttatg
     1741 ttcaattgtt tctccagtgc ctgtttttca cttaaaccaa tgcatccaat ttcaggaatt
     1801 gtaaaaattg attctggaat attttcataa gaaatcgctt catgttttcc agcgcagttt
     1861 gatgcagcaa tttctccttc ttttgtcgca acataagcaa gttgccatct acctgtcaca
     1921 tcgccagcag caaaaatgtt gtcaatgttt gtcttcatcc gttcatcagt aacaattcct
     1981 cctgctgcca gagtaattcc aatttcgcct gctccagtag catctggttt tcttccagtt
     2041 gcccacagta ttttttcttc gctgattgca tcagtggaat ttattttaat ccctgttttt
     2101 atctggatac ccttttgagc aaattgtttc tctatgactg aaataatatc tttatctttc
     2161 cctggtagaa tttgctccat catttcgtaa attgtaattt ttactccaaa gcaggaaaga
     2221 attgatgcaa actcgcatcc aataggacct gctccaataa ttgcaaggga ccctggaagt
     2281 ttttcaaggt tccagattgt atcagtggta ataattcttt cgtcgttaca aaatattgca
     2341 ggaacatttg gtcgcgcgcc tgtagcaatc acaatgtttt ttgctttaag tgaattgcca
     2401 ttcgctacaa ctgactcatt gtcgcacagt tttgcttctg cctggatgta ctgaactcca
     2461 ttagccctca atagcatccc aacacctgta ttcaatcgtg aaacaacatt tgtctttaca
     2521 tgattgagaa ttttctgcca gtcaaaagag atttcggcat tgataccatc aattcttggt
     2581 ttttgaatag ttcttgcaat cttaaataat gtctttgttg gaatgcaacc tgtattcaaa
     2641 caacacccac ccactttttc cttttcaatt agggctgttt tcaatccata ctgagcacat
     2701 cttattgcgc atggataacc tgcaggtcct ccaccaataa taataacatc aaactcattc
     2761 attgtgcctc ctgttcctta tattttaaca tcaaaaccca gatttttgtt tgaaatcgtt
     2821 tttattacag gtccaattcc tttacaatcc cttattctat cctccccttt gtaaaaatcc
     2881 cctcttactt cagggctcct tcctttataa cccctttttc tatccccctc tttataaaag
     2941 aggggctggg ggagatttca caaaatcccc ctttctcccc cttttgaaaa gggggagaat
     3001 gtgagtggaa attttataaa gggggatatc aggagagaga ttttgtaaag ggggagaaca
     3061 agagaaggga ttttacaaag ggggagaaca gaaagaagaa ttttgaaaag cgggaaatgt
     3121 ggaaagagca tcgcgcttgg tgggagtcgg cgccctcgcc tcccactacg gctcgggtcg
     3181 atccgccctg cctcaccgac tcggcaggct tgccatacaa tgctctttat tcatcgcatt
     3241 ggcaaccagc cgcacaggtt ctcgcttgct cgaacctata cgtcttcgct ccattcgact
//
LOCUS       DAORYU010000161         3293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_19348_length_3293_cov_4.051575, whole genome shotgun sequence.
ACCESSION   DAORYU010000161 DAORYU010000000
VERSION     DAORYU010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19348_length_3293_cov_4.051575"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PLW07_05545"
     CDS             complement(<1..290)
                     /locus_tag="PLW07_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOL50024.1"
                     /translation="MKNVNALLEILANEYPDTKTALNFQTPFQLLVATILSAQTTDIQ
                     VNKITPQLFKKFPTPEDFCNADLKEIQNSISSINFYKNKAKNIKLAAQKIVK"
     gene            337..1401
                     /locus_tag="PLW07_05550"
     CDS             337..1401
                     /locus_tag="PLW07_05550"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOL50025.1"
                     /translation="MAFVINLNIMKNTVKWILISGLFVFCFSGCRHAIEKRIHTISIV
                     AVGDINFGSDYPINKTPDKNQRKLLFRHCKDIIKNADIAFCNLETVLCRNGVPAKNIN
                     GKNTFVFRVDPDFCDVLVDAGFDVVSITNNHIRDFGTYGESQTKKILTQSGIQFVSID
                     GDIAEFVVSGVKICFVGFTSGLRNRSIVNPSVIFHEIKKLSEKYDILVVSFHAGAEGD
                     NAIHTKNTTEIFLGENRGNPVLLAHGAIDNGADLVLMHGPHVPRAVEMYKDRFIAYSL
                     GNFVNYGWNLSKFSKIAPLLWIELFSDGRACKVHIVSFIQRKSGFPEFDTGNSTYTII
                     KKLTESDFESGNIKFTNREE"
     gene            1402..>3293
                     /locus_tag="PLW07_05555"
     CDS             1402..>3293
                     /locus_tag="PLW07_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50026.1"
                     /translation="MKKFCLILAMFLILSGGIAMGFELAIEAEHFKLSGGWKVVPGGY
                     FPSQPNIWSLNLIMADETDGRAVATKVVEIPETKKYRLWVRYESCYGFGSVFKISVIQ
                     NGKIKAEQVFDRKNDQKYFPFNKGYTQQLAWFWHSTDFCYQGMDAELEKGSATIVISK
                     DKNEKPAAKRVIDLVYITDDLSIVPGNDWNWRGANEPPIISKFKKPIYVRVTCLEGSA
                     SVSVQTSLYLIGYYKGPRDIYYALKDKLSKDKPKKGELLKSGESTGWQQIMVSTVMSP
                     EISISKKGEGKVSVEIASEKPEDVVKKIELNQPEQNCSVIVAIGKQRYEEGLLGKHKA
                     LTVEEILQKQKKILDDYKVSGSPAKKLLIAFGLGKQYLKEQFDLAIACGANAAAYSSQ
                     PEIFGVNPTLKGFDTTVGALSVQNAHMTKECYQGNFASLEEMYKKIAQDMESTLGRKI
                     PYRIKLIEESGPPSFETLMTYDGLKQQYIDYLKSEGLNLDDVEKDSDIAFYHQHRFRA
                     LIFAKLNAQATKLIEKYFPEGTRTNSGSFYPSTGSMPTLARGDDPFLLFKERGVTEFS
                     SEISWGWGGTPDYIGPQTQSYEAALARALSKYHNCPMGSYLIADGNRGYTGDYVELAS
                     YPMYTQ"
BASE COUNT         1086 a          487 c          699 g         1021 t
ORIGIN      
        1 tctttcacaa ttttctgtgc cgcaagtttt atattttttg ccttattttt gtaaaaattg
       61 atggatgaaa tcgaattttg aatctctttc aggtctgcat tgcaaaaatc ttctggtgtc
      121 gggaattttt taaatagttg tggcgtgatt ttattgacct ggatatctgt ggtttgggca
      181 gaaagtatcg ttgccaccag aagttgaaat ggtgtttgaa aatttaaagc ggtttttgtg
      241 tcagggtact cattagcaag aatttcaagc aaggcattaa cgtttttcat aatgttattc
      301 tatcagattt tttgtttgtt ttcaacagga aattgtatgg cttttgtgat aaacttaaac
      361 attatgaaaa ataccgtgaa atggattttg atatctggat tgtttgtttt ttgtttttct
      421 ggatgcaggc atgctattga aaaaagaata cacacaattt ctattgtagc agtcggagat
      481 ataaattttg gttctgacta tcctattaac aaaactcctg acaaaaatca aagaaaattg
      541 cttttcagac attgtaagga cattataaaa aatgctgata ttgctttttg caatcttgaa
      601 acagtgctgt gcagaaatgg ggtgcctgca aaaaatatca atggaaaaaa tacattcgtg
      661 ttcagggtag accctgattt ttgtgatgtt cttgttgatg ctggttttga tgttgtttct
      721 attacaaaca atcatatcag ggatttcggt acatacggag aatctcaaac aaaaaaaatt
      781 cttactcaaa gcgggattca atttgtttca atagatggag atattgcaga atttgttgta
      841 tctggcgtga aaatttgttt tgtgggattc acatctggtc taagaaaccg ttcaattgtt
      901 aacccatcag ttatttttca tgaaataaaa aaactttctg aaaagtatga tatcctggtt
      961 gtttcttttc atgcaggagc agaaggggat aatgcaattc atacaaaaaa tacaactgaa
     1021 attttccttg gtgaaaatag aggtaatcca gtattgcttg cgcatggagc aattgataat
     1081 ggggcagatc ttgttttaat gcatgggcca catgttccaa gggctgtaga aatgtataaa
     1141 gatagattta ttgcttacag tttgggaaac tttgtcaact atggctggaa tctgagtaaa
     1201 ttttcaaaaa ttgcgccatt attatggatt gaactttttt ctgatggtag agcctgcaag
     1261 gtgcatattg tttctttcat tcaacgaaaa agcggatttc cagaatttga cacaggcaat
     1321 tcaacatata caataatcaa gaaattgaca gaatctgatt ttgaatctgg aaacattaaa
     1381 tttacaaaca gggaggaata agtgaaaaaa ttttgtttaa ttttagcaat gtttttaatt
     1441 ctttcagggg gcattgcaat gggatttgaa ttggcgattg aagcagaaca ttttaaactt
     1501 tcaggtggct ggaaggttgt accaggtggt tattttccat cacaaccaaa tatatggagt
     1561 ttgaacttaa tcatggcaga tgaaaccgat ggacgagcag ttgctacaaa ggtcgttgag
     1621 attccagaaa caaaaaaata caggttgtgg gtaagatacg aaagttgtta tggttttggt
     1681 tctgtattca aaatatcagt cattcaaaat ggtaagataa aggcagaaca agtatttgat
     1741 agaaaaaatg atcaaaagta cttcccgttt aataagggtt atacacagca acttgcctgg
     1801 ttctggcata gtactgattt ctgctatcag gggatggatg cagaacttga aaagggttct
     1861 gccaccattg ttatttcaaa agataaaaat gaaaaacctg ctgcgaaaag ggttattgac
     1921 cttgtgtata taacagacga cctttcaatt gttccaggaa atgactggaa ctggaggggc
     1981 gctaatgaac caccaattat ttcaaaattc aaaaagccca tatatgtaag ggtaacgtgc
     2041 cttgaagggt ctgccagtgt gagcgttcaa acatcattgt acttgattgg ttattacaaa
     2101 ggaccacgag atatttatta tgccttaaag gataaattat caaaggataa acccaaaaaa
     2161 ggtgaactct taaaatcagg cgaaagtact ggctggcaac agataatggt atcaaccgta
     2221 atgtcgcctg aaatcagtat ttctaaaaaa ggtgaaggaa aagttagtgt tgaaattgcc
     2281 agtgaaaagc cagaagatgt tgtgaaaaaa attgaactca atcaaccaga acagaattgt
     2341 tctgtgatag ttgctattgg gaaacaaagg tatgaagagg gattgcttgg taaacacaaa
     2401 gccctaactg tagaagaaat tttacagaaa caaaagaaaa tacttgatga ttataaggtt
     2461 tcaggaagtc ctgcaaaaaa acttctcata gcgtttggac ttggaaaaca gtatttgaag
     2521 gagcagtttg accttgcaat tgcctgtggt gcaaatgctg ctgcatatag cagccaacca
     2581 gaaatttttg gagtaaaccc tactttgaag ggatttgata cgacggttgg tgctttaagt
     2641 gtacagaatg ctcatatgac aaaagaatgt tatcagggta atttcgcctc tcttgaagaa
     2701 atgtacaaga aaattgcaca agatatggaa agtacgcttg gaagaaaaat cccgtacaga
     2761 attaaattga ttgaagaatc aggtccaccc tcttttgaaa cactgatgac atatgatgga
     2821 ttaaagcagc agtatattga ttatttgaaa tctgaaggat tgaatctcga tgatgttgag
     2881 aaggattcag atattgcttt ttatcaccaa catcgcttca gagcactgat atttgcgaaa
     2941 ttgaatgctc aggcaacaaa gttaatagaa aaatactttc ctgaaggaac aagaaccaat
     3001 tcgggtagtt tctatccttc aactggttct atgccgacgc tcgctagggg agacgatcca
     3061 tttttacttt ttaaggagag aggcgtaaca gagttttcaa gtgaaatttc ctggggatgg
     3121 ggtggaacac cagattacat aggaccgcaa actcaatcat atgaagcagc tcttgcaagg
     3181 gcactaagta aataccacaa ttgtccgatg ggttcttatt tgattgcaga tggtaatcgt
     3241 ggttatacag gggactatgt tgaacttgcc agttatccaa tgtataccca gga
//
LOCUS       DAORYU010000162         3277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_19464_length_3277_cov_4.637803, whole genome shotgun sequence.
ACCESSION   DAORYU010000162 DAORYU010000000
VERSION     DAORYU010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19464_length_3277_cov_4.637803"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="PLW07_05560"
     CDS             complement(<1..709)
                     /locus_tag="PLW07_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50027.1"
                     /translation="MKKSVSITWKQGKQTGTIEVKHGIVSEINIDGRSIEGKSFSFDA
                     DGKTRLNVTVEWQNKTGHAGPLIHVFSKNNPFSFLVSDVDEHFPVFVPEYNVIVTTCK
                     DTRTYHEIEYDVRKKNGLSKLKQMELEDDQDYASAEEKARKLRGYVWLGISRDIRIFE
                     IIFPDPDRTTLNVVPKYHGYGVKLPEFETLDESTRRWTGDEISYSVNFGRGSGYSQEL
                     ERYLEDRRIPILCGNLKD"
     gene            complement(734..2089)
                     /locus_tag="PLW07_05565"
     CDS             complement(734..2089)
                     /locus_tag="PLW07_05565"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOL50028.1"
                     /translation="MEKLAIEGGKKARTKETKEGAKYGIEELMELVKLWGLKPDTEEV
                     VRSALEKAWTGRSPHLFRYYHPDGDSMTRRFEIECSKFFGVPYVLATNSCTSSLIAAL
                     VAAGVGPGTEVIVPAHTFFASASAIVVAKAIPVIAEIDDSHTIDPIDVERKITPQTRA
                     IIVVHIKGLPCNMDAIMDIARKYNLVVVEDVAQAAGGWYKGKRLGTIGDIGCFSLDHY
                     KVIASGEGGIMTFKEEWFYTRAQSYHDTAACWRPNRYERERKEGELFAGENYRMSELQ
                     AAVAYAQIKKLGNIVENLRKSYKKIVSKLDLPEGVRTLKDNDPDGGCRYVLGLLWQDK
                     EMANAARDALKAEGIWGSTHDTDVRDWHVYRYWEHILEKKTATKEGCPYTCPYYKGKL
                     PDYSPDMCPNTIDYLSRSLFVGISPHFSDEDCQQIAHGINKVAKILAERKKKNQALLH
                     V"
     gene            complement(2143..>3277)
                     /locus_tag="PLW07_05570"
     CDS             complement(2143..>3277)
                     /locus_tag="PLW07_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50029.1"
                     /translation="QFIPDIKNQDWLRPILWSLICQCLQMFVKDEHNIARPRQGGRWA
                     GVWPVEAMEFLIAFDLLGLFHWSEKGYRFFVKHQEKDGEHKGRFNSYLSAQWENITGG
                     VLYGLSQHLLLHKDRNCFDEFRQAMVDGFNWIRERRKKSPNGLFPAGRPHDWGLTVQS
                     WCFTDAINVMGVSEMAKAFSLFGDPDAEKIKKEAEEYKNCMEKMLNEIIEQQKERDEI
                     FIPNYLGIPEKYPPVGPYFADGPTMLIRAGLIEPESEIFEKVERYFRNRGWMKNGLTG
                     LMTDGLLGSYASDPWAGHTWYVSFSDLCWFEGWLKRGEKEKAFETMWAQVRYGMSEHY
                     YMLERFADNDPSFCPWQPNASANGRLIQMLFKFFGTKTFTREM"
BASE COUNT          869 a          805 c          513 g         1090 t
ORIGIN      
        1 ggtccttcaa attgccgcac aaaattggaa ttcttctgtc ttcaagatat ctttcaagtt
       61 cctggctata accgcttccc cttccaaaat tgactgagta tgagatttca tcgcctgtcc
      121 atcttcttgt gctttcgtcc agtgtttcaa attcaggaag tttcacacca tatccatgat
      181 actttggtac aacattcaat gttgttctat cagggtcagg aaagataatc tcaaaaatcc
      241 tgatatccct gcttattcca agccagacat accccctgag tttccttgcc ttttcttctg
      301 cagatgcata atcctgatca tcttcaagtt ccatctgctt taatttactc aatccatttt
      361 tctttctaac atcgtattca atctcatgat atgttcttgt gtctttgcag gttgtcacaa
      421 tcacattgta ttctggaaca aaaacaggga aatgttcatc aacatcagaa acaagaaaac
      481 tgaatggatt gttttttgaa aaaacatgga taaggggacc tgcatggcct gttttgttct
      541 gccattcaac agtcacattg agccgcgttt ttccgtcagc atcaaaggaa aaactttttc
      601 cttcaatgct tctaccatca atgtttattt ctgatacaat accatgtttt acttcaattg
      661 taccagtttg ttttccctgt ttccaggtaa ttgaaacgga ttttttcatg tgcgcccttt
      721 ttattttcag catttacaca tgaagaagcg cttgattttt ctttttcctt tctgcaagaa
      781 tttttgcaac cttgttaata ccatgtgcaa tctgctggca atcttcatca gaaaaatgcg
      841 gactgatgcc aacaaacaaa ctcctgctca aataatcaat ggtattggga cacatatcag
      901 gcgaataatc aggaagtttt cctttgtagt atggacatgt gtaggggcaa ccttcttttg
      961 ttgctgtctt tttctcaaga atgtgctccc agtatctgta aacatgccag tccctgacat
     1021 cagtatcatg ggtactaccc catattcctt ctgccttcaa agcatccctt gcagcatttg
     1081 ccatttcttt gtcctgccac aaaagtccca gcacatatct acatccacca tcagggtcat
     1141 tgtcctttaa cgttcttact ccttcaggca aatcaagttt tgacacaatt tttttgtaac
     1201 tttttcttaa attttcaaca atgttaccga gttttttaat ctgcgcgtat gcgactgctg
     1261 cctgaagttc actcatcctg taattttcac ctgcaaacag ttcgccttct tttctttctc
     1321 tttcgtatct atttggtctc cagcatgcag cagtatcatg ataactctga gcccttgtat
     1381 agaaccattc ctccttgaat gtcataattc cgccttcacc gcttgcaatc accttgtaat
     1441 ggtctaaact gaaacatcca atatctccaa ttgtgcccaa tctttttcct ttgtaccagc
     1501 cacctgctgc ctgggcaaca tcttctacca caacaagatt gtactttctg gcaatatcca
     1561 taatagcgtc catattacag ggaagccctt taatatgaac aacaatgatt gccctggttt
     1621 gcggagtaat ttttctttcc acatctatcg ggtctatcgt gtgtgaatca tcaatttctg
     1681 caatcacagg tattgccttt gccacaacaa tggctgatgc tgatgcaaaa aatgtatgcg
     1741 caggaacaat cacttctgtg ccaggaccga cacctgctgc aacaagcgct gcgatcagtg
     1801 atgatgtgca ggaattagtc gccagcacat acggcacacc aaaaaacttt gaacactcaa
     1861 tttcaaacct tcttgtcatg gaatcgccat caggatgata atacctgaat aaatgcgggg
     1921 acctgcctgt ccatgctttt tcaagtgcac ttcttacaac ttcttctgta tctggcttta
     1981 atccccatag tttcacaagt tccatcaact cttcaatccc gtattttgcg ccttcttttg
     2041 tctcttttgt tcttgctttc ttgccacctt caattgcaag tttttccatg cttagcctcc
     2101 attttatgaa aaaataaggg ttaaaggtaa aaaactttaa tgttacattt ctcttgtgaa
     2161 tgtttttgta ccaaaaaatt taaataacat ctgaattaat cttccgtttg cgctggcgtt
     2221 tggctgccac ggacagaaac tcgggtcatt atcagcaaac ctttcaagca tgtaataatg
     2281 ctcactcatc ccgtatctta cctgtgccca cattgtttca aatgcctttt ctttttcacc
     2341 ccttttcaac cagccctcaa accagcataa atcagaaaaa gaaacatacc aggtatgtcc
     2401 tgcccacggg tcagaagcat aagaacccaa aagtccatct gtcatcaatc ctgtcagtcc
     2461 atttttcatc cagcccctat ttctgaaata cctttcaact ttttcaaaaa tttcactttc
     2521 tggctcaata agtcctgctc tgattaacat tgtgggtcca tcagcgaaat atggaccaac
     2581 aggcggatat ttttctggaa taccaaggta gttcggaata aaaatctcgt ctctctcttt
     2641 ttgctgctct ataatttcat tcagcatctt ttccatacag tttttgtatt cttctgcttc
     2701 tttctttatc ttttcagcat caggatcacc aaacaatgaa aatgcctttg ccatctcact
     2761 gacgcccatc acattgatgg catcagtgaa acaccaggat tgaacagtca atccccagtc
     2821 atggggtctt cctgcaggaa acaaaccgtt tgggcttttc tttcgcctct ctcgtatcca
     2881 gttaaaacca tcaaccattg cctgcctgaa ttcatcaaaa caatttctat ctttgtgcaa
     2941 aagaagatgc tgtgaaagtc cgtataaaac tcctcctgtg atgttttccc attgcgcaga
     3001 cagatacgaa ttaaatcttc ctttatgttc tccgtctttc tcctggtgtt ttacaaaaaa
     3061 cctgtatcct ttttctgacc aatgaaataa accgagtaaa tcaaacgcga taagaaattc
     3121 cattgcttca acaggccaga cacctgccca tcttcctccc tgacgcggtc ttgctatgtt
     3181 atgttcatct tttacaaaca tttgcaaaca ctggcatatc aatgaccaca ggattggcct
     3241 caaccagtcc tgatttttga tatcaggaat gaattgc
//
LOCUS       DAORYU010000163         3271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_19519_length_3271_cov_4.984764, whole genome shotgun sequence.
ACCESSION   DAORYU010000163 DAORYU010000000
VERSION     DAORYU010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19519_length_3271_cov_4.984764"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1663)
                     /locus_tag="PLW07_05575"
     CDS             complement(<1..1663)
                     /locus_tag="PLW07_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50030.1"
                     /translation="MERVFTFRSFLTGIILSIFIAFYDHVSVDINSGSYLAIDHMPVA
                     AITLFFFLVFFVNTLLKRIKQKLAFGAGELLLVYCMMLVACSVTEMGIGSQILPLISA
                     PSYYANPQNEWHNLIVPHLRKSLIVTDPDAVTYFFEGLPKGMSIPWGAWIKPLSLWIP
                     FLLVFYFCTICIAVMIRKQWMDREKLVYPLTILPTEMVKEQPVPQGKRSYLPGFFKNS
                     LMWLGFGIAFIVGTMVALHAYNNMIPAPKLQQFITTFRGTQNIVFRVSFPVIGFVYLA
                     NLEVSFSLWFFSLIFQVIKGVFNITGISSTENIGIYGCSGYAIFAHLGTGAMMAMVIY
                     SLYIARSHFRDIWRSAIGKANIDDSGEILSYKTAFWGFIIGSIVVLGWLKYSGLSITM
                     GLVFYLFALIIFLVLTRIVCEAGVPTLVATIISSSVVISMWGSKHMPVTALVALGLTY
                     VYSADLRTFPMAASSMSLKIMDKFSRGKRYFFWAIMAAILVNLIATLFFILKISYKYG
                     GINLNSWFFRGGPQAPFDYIADLIKNPTDSNKIGWLCRGIGLAVMS"
     gene            complement(1666..3108)
                     /gene="gatA"
                     /locus_tag="PLW07_05580"
     CDS             complement(1666..3108)
                     /gene="gatA"
                     /locus_tag="PLW07_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HOL50031.1"
                     /translation="MDINCLSLHQIKELLKKREISPVELVRKQLEIIHDKNKILNAFL
                     YINDSCLEQAKQSEQRYISGEFRELDGIPVAIKDNICTIDQPTTCASRILEDYMSPFD
                     AHVIEKIRNSGAILVGKTNMDEFAFGSSTENSAYGPALNPYDTTRVPGGSSGGSAVSV
                     AANMAFGALGSDTGGSIRQPASFCGVVGLKPTYGRVSRYGLVAFGSSLDQIGPFARNV
                     RDASIIMRMIAGHDAHDSTSCTIPVPDYEKELEKPLDKKNIRIGIPDEYFGQGLSKEI
                     KDKIDGIIKRLASDGFNVERIRLPHTNYGIPTYYIIGTAEASTNLARFDGVKFGYRSK
                     HAKTVFDVYTNSRGEGFGNETKKRIILGTFVLSAGYYDAYYLKAQKVRSLIIKDFQQA
                     FQSVDFIITPTTPETAFKIGEKAKNPLAMYLSDVFTVNANLAGIPGLNLPVGFSSDKL
                     PFGMQILAPAFKESELLGFAYYIEQNYTKE"
     gene            complement(3110..>3271)
                     /locus_tag="PLW07_05585"
     CDS             complement(3110..>3271)
                     /locus_tag="PLW07_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HOL50032.1"
                     /translation="QGVPSDGEIVISTANRNFKGRMGNPNAFIYLASPATVAASAIKG
                     EISDPREFL"
BASE COUNT          972 a          705 c          658 g          936 t
ORIGIN      
        1 cagacatcac tgccagtcct atcccgcgac acagccatcc gattttgttt gaatcagtag
       61 ggttttttat caggtccgca atgtagtcaa atggtgcctg tggaccacct ctgaaaaacc
      121 agctgttcag gttgattccg ccatacttgt acgaaatttt gagtataaaa aacaatgtag
      181 caatgagatt gaccagaatc gctgccatga ttgcccagaa gaaatatctt tttcctctac
      241 tgaacttatc cataattttt aatgacatgc tgctggctgc cattggaaat gttcttaaat
      301 cagcactgta cacatatgtc aaccccagag ccacaagtgc tgtgacaggc atatgcttgc
      361 ttccccacat ggaaatcacg acggatgacg atatgattgt tgcaacaagt gttggaacac
      421 ctgcctcaca cacgattctt gtcaaaacaa ggaaaatgat cagcgcaaac agataaaaga
      481 caagacccat cgtaatactg agtcctgaat acttcagcca gcccagcaca acgattgaac
      541 cgataataaa tccccagaaa gcagttttgt aagaaaggat ttcaccagaa tcatcgatat
      601 tggcttttcc aattgcagag cgccatatgt ctctaaaatg gctgcgggca atatacaaac
      661 tgtatattac catcgccatc attgcgccgg ttccaagatg ggcaaatata gcatatccag
      721 agcatccata aattcctatg ttttctgtgc ttgagatacc tgtgatattg aaaacgccct
      781 tgataacctg aaaaatcaaa ctgaaaaacc ataaggaaaa actcacctca aggttcgcta
      841 aatacacaaa accaatgact ggaaaactta ccctgaatac gatgttttga gttcctctga
      901 atgtagtaat aaattgctga agttttggtg caggtatcat attattgtac gcatgcagtg
      961 ccaccatggt tcctacaatg aatgcaatgc caaaaccgag ccacatcagg gaatttttga
     1021 aaaagccagg caggtatgac cttttaccct gtggaacagg ttgttccttc accatttctg
     1081 tgggaagtat tgtcagtgga tataccagtt tctccctgtc catccactgt tttcgtatca
     1141 tcacggcgat gcatatcgtg cagaaataga aaacaagtaa aaaaggtatc cataatgaaa
     1201 gaggtttaat ccaggcaccc catggaatag acattccttt tggaagacct tcaaaaaaat
     1261 atgtgactgc gtcaggatct gttactatca aactttttcg caaatgtgga acaatgagat
     1321 tgtgccattc attctgggga tttgcatagt atgatggagc agaaatcagt ggaagaatct
     1381 gactgccaat gcccatttca gtcacagagc aggcgaccag catcatacaa tacacaagaa
     1441 gtaattctcc tgctccgaaa gcgagttttt gctttattct tttgagcaac gtattaacaa
     1501 aaaacacaag gaagaaaaac agggtaatgg ctgccactgg catgtggtca atggcaagat
     1561 aagaaccgct gttgatatct actgaaacat gatcataaaa cgcaataaat atggaaagta
     1621 tgatacctgt caggaaagag cgaaaagtaa atacccgttc cacgttcact cctttgtgta
     1681 gttctgttca atgtaatatg caaaaccaag tagttctgat tccttaaatg caggcgcaag
     1741 tatctgcata ccaaaaggaa gtttatcaga agaaaagcca actggcaaat ttaatcctgg
     1801 aataccagcg aggtttgcgt tcacagtgaa aacgtctgac agatacattg caagtggatt
     1861 tttggctttt tcgccaatct tgaatgctgt ctctggcgtg gtgggagtga tgatgaaatc
     1921 tactgactga aatgcctgtt gaaagtcctt gatgatcagt gaacgaacct tctgcgcttt
     1981 tagatagtat gcatcgtagt aacccgcaga aagaacaaag gtgccaagta tgatgcgttt
     2041 tttggtttca tttccaaaac cctctcccct ggaatttgtg tacacatcga atactgtttt
     2101 agcatgtttg cttcgatatc caaacttgac gccatcaaac cttgcgaggt ttgtactcgc
     2161 ttcagcagtt cctatgatgt agtatgtagg tatcccgtag ttggtatggg gtaatcttat
     2221 tctttccaca ttgaagccat cgctggcaag acgcttgata attccatcaa ttttatcttt
     2281 tatctctttt gaaagtccct gcccgaaata ttcatcaggg attcctatgc ggatgttctt
     2341 cttatcaaga ggcttttcaa gttccttttc ataatcaggg acagggattg tgcatgaagt
     2401 tgaatcgtgt gcatcgtgtc cggcaatcat tctcatgatg atacttgcat ccctgacatt
     2461 tctggcaaaa ggacctatct ggtcaaggga tgaaccaaat gcaaccagcc cataccttga
     2521 aaccctacca taagtgggct ttaaacccac aacaccgcag aaacttgcgg gttgcctgat
     2581 tgagccaccc gtatcagagc caagcgcgcc aaatgccata tttgctgcaa cagatactgc
     2641 agaaccaccg cttgaaccac caggaaccct tgttgtgtca tacggattca aagcagggcc
     2701 ataagcagag ttttctgtgg atgagccaaa tgcaaattca tccatattgg ttttacccac
     2761 aagaattgcg cctgagtttc tgattttttc aatgacatga gcgtcaaagg gactcatgta
     2821 atcttcgaga atccttgaag cgcaggttgt tggctggtct attgtgcaga tattgtcctt
     2881 gattgctaca ggtatgccgt caagttccct gaattcacca ctgatatatc tttgttcact
     2941 ttgctttgcc tgttcaagac aactatcatt gatatagaga aaggcattca gaattttatt
     3001 tttatcgtga ataatttcta attgttttct gacaagttca acaggcgaaa tctctcgttt
     3061 ttttaagagt tcctttatct gatgtaaact caaacaatta atgtccatat cataaaaatt
     3121 ctctcgggtc tgaaatttcg ccctttattg cagaggcggc tactgttgcc ggagaggcaa
     3181 gatagataaa cgcatttgga ttacccatgc gtcccttaaa atttctgttt gctgttgata
     3241 tgacaatttc gccgtctgat ggaactccct g
//
LOCUS       DAORYU010000164         3255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_19636_length_3255_cov_7.368437, whole genome shotgun sequence.
ACCESSION   DAORYU010000164 DAORYU010000000
VERSION     DAORYU010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19636_length_3255_cov_7.368437"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="PLW07_05590"
     CDS             complement(<1..747)
                     /locus_tag="PLW07_05590"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF014067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HOL50033.1"
                     /translation="MILEKQQAKKGIITDAVKNVASIEQISPEKLAVSIAEGKTVILK
                     HKNTCVGIGENLKVKVNTNIGTSSDVCDIDFELEKLKTACRLKSDAVMDLSTGGDLDS
                     IRKMIINASSIPVGSVPIYQAMIQAIEKYGTIEKLKEEEIFQVIEKHAASGISFVTVH
                     CGVTLSSLELLRKKSRRTGIVSRGGAFIASWMVSAGKENPLYANFDRLIEIARKYDLV
                     LSLGDGLRPGCLADASDNLQMSELLTLGKLT"
     gene            complement(744..2411)
                     /gene="ilvD"
                     /locus_tag="PLW07_05595"
     CDS             complement(744..2411)
                     /gene="ilvD"
                     /locus_tag="PLW07_05595"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOL50034.1"
                     /translation="MISDQVKKGYVRAPHRSLLKATGLTDQEIERPLIGICNSGNEII
                     PGHIHLDSVCQATKSGVRMAGGTPIEFRTIGVCDGIAMGHEGMKYSLVSRELIADSIE
                     IMAKSHGFDGLVLVASCDKIIPGMIMAALRLNLPAILISGGPMLAGVCPGAGTVDLIS
                     VFEAVGKVSSGEITEQQLKILEDYACPTCGSCAGMFTANSMNCLAEVLGIALPYNGTI
                     PAVHSARIRLAKQAGMKIMEMVKNNVSIKDITTMKSFENAIAVDMAIGASTNTVLHLP
                     AIAHEAGIDLTLDLFDSISKKIPNLCKISPAGSHHMEDLDRAGGIPAVMKELCQADLL
                     HKNEKNVSGKTIGEIAEAAKNFDTSVIRKVSDPYSKEGGIAILKGNIAPDGSVIKQSA
                     VVSGMMVHKGPAKVFNGEEDCLSAVLEGKINKGDVIVIRYEGPRGGPGMREMLAVTSA
                     ISGKGLGEYVALLTDGRFSGGTRGACIGHISPEAAEGGPIGCIQDGDIIEINIPDRKL
                     NVLVSDQEISDRLKKIRHPEKKLSGILARYRAQVKSASTGAILKDTI"
     gene            complement(2427..>3255)
                     /locus_tag="PLW07_05600"
     CDS             complement(2427..>3255)
                     /locus_tag="PLW07_05600"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOL50035.1"
                     /translation="NDIIDLNARSVSGIINRGGTILRTIRFPELSKSDVQATCIENLR
                     KGNIDALVVIGGDGSSRGAFALHQHYGFPVCVIPASIDNDVYGTDYTIGFDTAVNTAL
                     EAIDRIRDTATSHERTFVIEVMGREHGFLALDVAIAAGAEIVMIPERPITINKVMEIL
                     EKDRVMGKISSLVIVAEGAGRAHEVAKEINQLMPGVEARYSVLGYIQRGGTPTYQTRC
                     LATMFGNAAVKGLIDGLTHFMVGIRGTEIVRVDLKEVVSKTKQISEEKLKLVETMAI"
BASE COUNT          890 a          816 c          532 g         1017 t
ORIGIN      
        1 agtaagtttt cccagcgtca gaagttcgga catctgtaaa ttgtcagacg catcagcaag
       61 acaccctggt ctcaatccat cacctaaact caacacaagg tcgtattttc ttgcaatttc
      121 aatcagtcta tcaaaatttg cataaagagg attttccttt ccagcactga ccatccagct
      181 tgcaataaat gcgccacccc gactaactat tccggttctt cttgattttt ttctcaacag
      241 ttcaagactg gacaaagtta ctccacaatg aaccgttaca aaactgatgc cactggccgc
      301 gtgtttttca ataacctgga aaatttcttc ctctttgagt ttttcaattg ttccatattt
      361 ttcaattgcc tgaatcattg cctgataaat cggaacagaa ccaacaggaa tggaactggc
      421 attgataatc atcttacgaa tgctatcaag gtctccgcca gtacttaaat ccattactgc
      481 gtctgatttt aatctgcagg cagtttttaa tttttcaagt tcaaaatcaa tatcgcatac
      541 atctgatgat gtaccaatat tggtattaac cttcactttc aaattctcgc caatcccaac
      601 acatgtgttt ttatgcttca atattactgt ttttccttca gcaatagaaa cagcaagttt
      661 ttcaggagaa atctgttcaa tagatgccac atttttcaca gcatctgtga taataccttt
      721 ttttgcctgc tgtttttcaa gtatcatatc gtatccttta gaatcgcgcc tgtggaagcg
      781 cttttcacct gcgccctgta cctcgcaaga attcctgata atttcttttc agggtgcctg
      841 atttttttca acctatcaga aatttcctgg tcagaaacaa gtacgttcaa tttcctatca
      901 gggatattaa tctcaataat gtctccatcc tgaatacatc caataggtcc gccttcagct
      961 gcttcaggac tgatatgtcc gatgcaggca cctcttgtac ctcctgaaaa tcgtccatct
     1021 gtaagaagtg ccacatattc tccgagtcct ttgcctgaaa tcgctgatgt tacagccagc
     1081 atttccctca taccaggacc gccgcgtgga ccttcataac gaatcacaat cacatctcct
     1141 ttatttattt ttccttcaag aaccgcagaa agacaatctt cctctccatt aaacactttt
     1201 gcaggtcctt tgtgaaccat cattccactt accactgctg attgcttaat gacactacca
     1261 tcaggagcaa tgtttccttt caatattgct ataccacctt cttttgaata agggtctgaa
     1321 acttttctga taaccgaagt atcaaaattt ttggctgctt ctgcaatctc tccgatggtt
     1381 ttgccactaa catttttctc atttttgtgc aacaaatctg cctgacacaa ctctttcatc
     1441 acagcaggaa ttccacctgc tctatcaagg tcttccatgt gatgactgcc tgctggtgaa
     1501 attttgcaaa gattcggtat ctttttcgaa atgctgtcaa ataaatccag tgttaaatca
     1561 atgcctgctt catgcgcaat cgctggaaga tgtaacacag tattggtaga agcaccgatt
     1621 gccatatcaa cagcaattgc attttcaaag gacttcattg tagtgatgtc tttaatactt
     1681 acattatttt ttaccatctc catgattttc atacccgcct gttttgcaag ccgtattctt
     1741 gcggaatgta cagcaggaat tgttccattg tatggtagtg cgattccgag aacctcagca
     1801 agacagttca ttgaatttgc tgtaaacatt ccagcacatg aaccgcatgt tggacaggca
     1861 tagtcctcaa gaattttcaa ttgttgctct gtgatttctc ctgatgatac tttaccaact
     1921 gcttcaaaaa ctgaaatcag gtcaactgtt cctgcaccag ggcacactcc tgcaagcatg
     1981 ggtccgccac tgataagaat tgcaggaaga ttcaatctga gcgctgccat aatcatacca
     2041 ggaataattt tgtcacagct ggcaacaaga acaagtccat caaagccatg ggattttgcc
     2101 ataatttcaa ttgagtccgc tattagttct cgagaaacaa gggagtattt cattccctcg
     2161 tgtcccattg caataccatc acaaacacca atggttctaa attcaatcgg agttccgcca
     2221 gccattctta caccactttt tgttgcctga caaactgaat caagatgaat gtggccgggg
     2281 ataatttcat tgccggaatt acagatacca atcaagggcc tttcaatttc ctggtctgtt
     2341 aaacccgttg ccttcagcaa acttctgtgc ggagctcgca cataaccctt tttcacctga
     2401 tcgctaatca tacttcctcc agtgtcttaa atagccatcg tttcaacaag tttcaatttt
     2461 tcctctgaaa tctgtttggt ttttgaaaca acctctttca aatcaaccct cacaatctct
     2521 gttccacgaa ttcccaccat aaaatgtgtc aatccatcaa taaggccctt taccgctgca
     2581 ttgccaaaca tcgtggcaag gcatcgtgtt tgatatgtgg gagtccctcc tctttgaatg
     2641 taacccagca cagaataccg cgcctcaaca ccaggcatca actgattaat ttcttttgca
     2701 acttcatggg ctctgcctgc gccttctgca acaataacca gagaactgat ttttcccatc
     2761 accctatctt tttcaagaat ttccataacc ttgtttattg taattggtct ttcaggtatc
     2821 atcacaatct ctgcacctgc tgcaatcgca acatccaggg ctaaaaaccc gtgctccctg
     2881 cccattacct caataacaaa tgttctttca tgacttgtcg cagtatcccg tatcctgtct
     2941 atggcttcaa gggcagtatt aactgcggtg tcaaatccaa ttgtataatc tgtgccataa
     3001 acatcgttgt caatagatgc tggaatcaca cacactggaa atccataatg ctgatgcaga
     3061 gcaaaagcac ccctgcttga accatctcca ccaatcacga ccagagcatc aatgttacct
     3121 tttcttaaat tttcaataca cgttgcctga acatcggatt tactcaactc cggaaatctt
     3181 atggttctta gaatcgtgcc gccgcggttg ataatgccac tgacactgcg cgcattcaaa
     3241 tcaatgatat cgttt
//
LOCUS       DAORYU010000165         3237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_19783_length_3237_cov_3.796669, whole genome shotgun sequence.
ACCESSION   DAORYU010000165 DAORYU010000000
VERSION     DAORYU010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19783_length_3237_cov_3.796669"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1262
                     /locus_tag="PLW07_05605"
     CDS             <1..1262
                     /locus_tag="PLW07_05605"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HOL50036.1"
                     /translation="KQVAGEKGIKIKNNTRICIAPEIIEQLLGPFPKNGDNNLGDKLS
                     FHVSGYALRCYDLRTGEIKKPTTTDMIEFTKIVHTLGVQGSSIVMPQDLPQKLAEVAT
                     YKLCLDVSDKVWGAGIFSDAEVFDIVQEILVAIGLPYTIGMHMISPLAFDPFLLEMAI
                     RYIPKKATFSVGNMPMRGATCPIDLASSLSQSCAEVLGGAAILKILAPDSRVSFAPFV
                     YPFDMRYATIVYGGPDFIIGNLVLNQIAKFYGTSVMAKACNTMAKKPDDAQAGINAAG
                     FIFMMLAGMKEFGWSGTCCIDEIGSVEKMIIDYEIFRFAKHIVEGFEFKDRGNLVEII
                     DECVKEGTFLAHQDTLNNYRKILFESDLFSNDNFMAWDREGRKSLLDRARTKAMKLLG
                     SHSFTRDKEQQKELDKIWEKAKQMFTA"
     gene            1276..2115
                     /locus_tag="PLW07_05610"
     CDS             1276..2115
                     /locus_tag="PLW07_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOL50037.1"
                     /translation="MRLGGPVLQKFETPEEWVKILKRNNYSAAYCPTGLDAPDELVKE
                     FKKMAEKESIVIAEVGAWSNPISGDENERRQAIEKCKRALYLAEKINARCCVNISGSR
                     GSKWDGPDEKNFTDETFDMIVMTTREIIDEIKPERTFYTLETMPWMYPDSAESYLRLI
                     KAIDRESFGVHFDPVNLVCSPQRYFSNEKLIKEFVKILGIYICSCHAKDIILTDKLTV
                     HLEEVRPGLGKLDYRVFLNEINKIGKDTPPVMLEHLRTEEDYVNAAQYIRTVASQEGI
                     KLL"
     gene            complement(2110..2964)
                     /locus_tag="PLW07_05615"
     CDS             complement(2110..2964)
                     /locus_tag="PLW07_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HOL50038.1"
                     /translation="MNKKVLLDTDIGSDIDDAICLAYLLSHPECDLIGITTVTGQPEK
                     RAMIASYLCEKAGRNIAIFPGMSEPLIGQQKQKFAPQYEFIEKITYKKSFQGEWFNFL
                     RNTIRQNPGEINLITIGPLTNIGILFKIDPELPRMLKSMYIMGGMFFGENKKVEWNIS
                     CDPFAAHTVFSSDVKQIFVCGLDVTLKVKMEKTEVIQTFNKNEILKEVLNFAEIWFRE
                     RDTVVFHDILAASALFKNTCSFKKGKVFVEQLGQTGFIEDENGNTFVASEVDEELFFS
                     HFFDTVMS"
     gene            complement(2957..>3237)
                     /locus_tag="PLW07_05620"
     CDS             complement(2957..>3237)
                     /locus_tag="PLW07_05620"
                     /inference="COORDINATES: protein motif:HMM:NF013798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HOL50039.1"
                     /translation="GPVYHHEKNPAEKLMMCFKNCLMLADSKGLKSIAFPAISTGIFG
                     YPLEEAAEVSIRAVIKTIKELKSLKKVRFVLFDEKSFSVFEQALKKYE"
BASE COUNT         1045 a          573 c          646 g          973 t
ORIGIN      
        1 taaaacaagt tgcaggagaa aaaggcatta aaataaaaaa caatacccga atttgcattg
       61 ctcccgaaat aattgaacaa ttacttggac cttttccaaa aaatggtgac aataatttgg
      121 gcgataaact atcatttcat gtttcgggtt atgccctgcg ctgttatgat ttgaggactg
      181 gcgaaataaa aaaacccact acaactgata tgattgaatt tacaaaaatt gtccatactc
      241 ttggagttca agggtcaagt atcgtaatgc cacaggattt accgcaaaaa cttgctgaag
      301 ttgcaacata taaactgtgc cttgatgtgt cagacaaagt atggggtgct gggatcttca
      361 gtgatgctga agtttttgat attgtacagg aaattctggt tgctatcggt ttaccataca
      421 ctatcggaat gcacatgatc agtccacttg cctttgatcc atttctactt gaaatggcaa
      481 tcagatatat tcccaaaaaa gcgacatttt cagttggcaa tatgcccatg agaggagcaa
      541 catgtccgat tgaccttgct tcttcattat cacagtcgtg tgctgaagtc cttggaggag
      601 ctgcgatatt aaaaatactt gcgccagatt caagagttag ttttgcgccg tttgtttatc
      661 cttttgatat gcgatatgca acaatagttt acggtggccc tgactttatc attgggaatc
      721 ttgttttgaa ccagatcgcg aaattttatg gaacatctgt gatggcaaag gcgtgtaata
      781 ccatggcaaa aaaaccggat gatgcacagg caggcatcaa tgcagcaggt tttattttta
      841 tgatgcttgc tggaatgaaa gagtttggat ggtccggcac ctgctgtatt gatgaaattg
      901 gttctgtgga aaaaatgatt atagattatg aaatttttag atttgctaaa catatcgtag
      961 agggctttga gttcaaagac aggggtaacc tggttgaaat cattgacgag tgcgtaaaag
     1021 agggtacgtt tttggcgcac caggatactc tgaataatta ccgaaaaatt ctttttgaat
     1081 cagatttatt ttcaaacgac aattttatgg catgggatag agaaggcaga aaaagtttgc
     1141 ttgacagggc aaggactaaa gcgatgaaat tacttggctc tcattcgttt acaagagaca
     1201 aagaacaaca gaaagaactt gacaaaatct gggaaaaagc aaaacagatg tttacagcgt
     1261 aacaggagaa ataaaatgag acttggcgga cctgtattac agaagttcga aacccctgaa
     1321 gaatgggtga agattttgaa aagaaacaat tattctgctg catattgtcc tactggactg
     1381 gatgctcctg atgaattagt taaggaattt aagaagatgg cagagaaaga aagtatcgta
     1441 atcgcagagg ttggagcgtg gagtaaccca atatcgggtg atgagaacga aagaaggcag
     1501 gcaatagaga aatgcaaaag ggctttgtat cttgctgaaa aaattaatgc tcgttgttgt
     1561 gtcaacatct ctggttcaag gggaagtaag tgggacggtc ctgacgaaaa gaatttcaca
     1621 gatgagacat ttgacatgat tgtgatgaca acgagagaaa tcattgatga aataaaacca
     1681 gaaagaacat tttacacgct tgaaacaatg ccatggatgt atcctgattc tgctgaaagt
     1741 tatctgcgac tcatcaaagc aattgataga gaatcctttg gggttcattt tgaccctgta
     1801 aatcttgtat gcagtccgca aagatatttt tcaaacgaaa aattgataaa ggaatttgtt
     1861 aaaatactcg gtatctatat ctgttcgtgt catgccaaag atattatact cactgataaa
     1921 ctaacggttc accttgaaga ggtacgaccg ggcctcggaa aacttgacta cagggtattt
     1981 ctaaatgaaa taaataaaat tggtaaagat actcctccag taatgcttga gcatttgcgg
     2041 accgaagagg actacgtaaa tgcagcacaa tacataagaa cagttgccag tcaggaagga
     2101 ataaaattgc tatgacatca ctgtgtcaaa aaaatgtgaa aaaaacagtt cctcatctac
     2161 ttctgaagca acgaatgtat tgccgttttc atcttctata aaaccagtct gacccagttg
     2221 ttccacaaaa acctttcctt ttttgaaact gcaggtattc ttaaataaag cactcgccgc
     2281 aaggatatca tgaaaaacaa cagtatccct ttccctgaac catatctcag caaaatttag
     2341 tacttctttc aaaatctcat ttttattaaa tgtttgaatt acctctgtct tttccatttt
     2401 tactttcaat gttacatcaa gcccgcagac gaaaatttgc tttacatcag aagaaaaaac
     2461 tgtgtgtgcc gcaaaagggt cgcaggaaat attccattca acctttttat tctccccaaa
     2521 aaacatgccg cccataatgt acattgattt cagcatgcgt ggtaattcag ggtctatttt
     2581 gaaaagaatt ccgatattgg ttaatggtcc tattgtgatt aaattgattt ctccaggatt
     2641 ttgcctgatt gtatttctta aaaagttgaa ccattctcct tgaaaggatt tcttatatgt
     2701 aattttctca ataaattcat actgcggagc aaatttttgt ttttgttgac ctattagtgg
     2761 ttcagacata ccaggaaaaa tagcaatatt tcttcctgct ttctcacata agtaacttgc
     2821 aatcatcgcc cgtttttcag gctggcctgt tactgttgta atgcctataa ggtcacattc
     2881 aggatgggat aacaaatacg caagacagat tgcatcgtct atgtcagaac caatgtctgt
     2941 atcaaggagt acctttttat tcatattttt tcaatgcctg ctcaaaaaca gaaaacgact
     3001 tctcatcaaa tagaacaaac ctgacttttt ttagggattt caattcctta attgttttaa
     3061 ttactgctct gattgaaact tcagccgctt cttctaatgg ataaccaaaa atgcctgttg
     3121 aaattgcagg aaatgcaata gattttagtc ctttactgtc tgccagcatc aggcagtttt
     3181 tgaaacacat cataagtttt tctgctggat ttttctcatg atggtacaca gggccaa
//
LOCUS       DAORYU010000166         3230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_19845_length_3230_cov_3.267402, whole genome shotgun sequence.
ACCESSION   DAORYU010000166 DAORYU010000000
VERSION     DAORYU010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19845_length_3230_cov_3.267402"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PLW07_05625"
     CDS             complement(<1..322)
                     /locus_tag="PLW07_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HOL50040.1"
                     /translation="MNQKEMKSFRDILIREKVRILKSLSQLEENTQQSARGNVSEGAG
                     VPTHIADLGTDAFEKDLDLNLTSSEIELLRMIEESLKKIDGKKYGICESCNKPISKAR
                     LKAIP"
     gene            complement(333..770)
                     /locus_tag="PLW07_05630"
     CDS             complement(333..770)
                     /locus_tag="PLW07_05630"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HOL50041.1"
                     /translation="MSQSSGKQKKFEYIEHPADIAIRVYGRNLQELFENAAMGLYDIL
                     KPQKKIKEPQYQREKTFYSDSKEGLLVCFLNELLFIALKEHLIFYKFLFNIDCSEKEN
                     KLVCVTTGHRSEGVEREVKAVTYHRMEIKKVRNFLQTEIIFDI"
     gene            complement(736..3129)
                     /gene="gyrA"
                     /locus_tag="PLW07_05635"
     CDS             complement(736..3129)
                     /gene="gyrA"
                     /locus_tag="PLW07_05635"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOL50042.1"
                     /translation="MRNSYLDYAMSVIIGRALPDVRDGLKPVHRRILYAMMELGLEPG
                     RPYKKSARIVGEVMGKYHPHGDSPIYESLARMAQDFTLRYVLVDGQGNFGSIDGDPPA
                     AMRYTEARLRPLAMEILADIEKDTVDFMPNFDESLVEPVVLPSKVPNLLINGSSGIAV
                     GMATNIPPHNLGEVCDALIMMIKNPDCTVEDIMKFVTGPDFPTGGTICGTQGIVEAYT
                     TGKGIITIRGKVEVEEAKNHDNVVITEIPYEVNKSTLVENIARLVEEKKIGGVSNVRD
                     ESSKEGIRIVLETRKGENTDIIINQLYSHTQLQTTYGIINLALVNRQPKLLTLKEILE
                     LFLEHRRNVVIRRTRFELNKAEERHHILLGLKIAVDNLDKVISLIRNSNSVEEARENL
                     MKKFSLSQKQADAILALPLGKLTKLERNKIEEELDQISKKIAELKAILASKEKVNAII
                     EGELKVLKNKYSDRRRTEIGSPIEELSDEDLVRPEDIVVTVSAHGYIKRLPLETYRRQ
                     RRGGRGILGAGTKEDDFVRHVFVCSTIDQLLIFTETGRVHWLKGYLIPEASRVARGKP
                     LVNLLKIQTEEKIAAVIPVPDFSSGQFILMLTKKGIVKKTPLIAYSKPRQGGIIGITL
                     KNDDKLIDVKLTDGDRDIMLFTKHGFCIRFSEQEVKPIGRTGMGVIGIRFKNPKEEVV
                     CGQVFVSEKPTEEILTVSSNGYGKRTYVKYYRRQKRGGKGVIDMKVNSKTGYVVKTKM
                     VQEDDEIIVMTMGGMIIRVPVNEIRKVGRSTLGVKLINLNQGDRIADVAIVRETEEI"
BASE COUNT          864 a          775 c          495 g         1096 t
ORIGIN      
        1 atggaattgc tttcagtctc gccttagaaa ttggcttatt gcaggattcg catatcccat
       61 attttttccc atcaattttc ttcaatgatt cttctatcat tcgaagcagt tcaatctctg
      121 aactggtaag atttaaatca aggtcttttt caaatgcatc agtaccaaga tccgcaatat
      181 gtgttggtac gccagctcct tcagatacat ttccgcgcgc actctgctgg gtattctctt
      241 caagttgaga aagacttttc aatatcctga ccttttccct gatcaggata tccctaaaac
      301 ttttcatttc tttttgattc acattacctc cttcaaatat caaatatgat ctctgtctgc
      361 aaaaaattcc taactttctt aatctccatg cggtgatagg taacggcttt aacttctcgt
      421 tccactcctt cacttctgtg tccagttgtc acgcacacaa gtttgttttc tttttctgaa
      481 caatctatat taaacaaaaa tttataaaaa atcaaatgtt ccttcagtgc aatgaaaagc
      541 aattcattca aaaaacaaac aagaagtccc tctttagagt cagaataaaa ggttttctct
      601 cgctgatatt ggggttcttt tatcttcttt tgaggcttca gtatatcata aagtcccatt
      661 gcggcatttt caaacagttc ctgcagattt ctcccatata ctcttattgc aatatccgct
      721 ggatgttcaa tatattcaaa tttcttctgt ttcccggacg attgcgacat ccgcaatacg
      781 atctccctga ttaagattta tcagttttac cccgagagtg cttcgtccaa cttttcttat
      841 ttcgttaaca ggtaccctga taatcattcc gcccattgtc atcactataa tttcatcatc
      901 ttcctgaacc atctttgttt ttaccacata accggtttta ctattaacct tcatatcaat
      961 aacaccctta ccacctcttt tctgtctcct gtaatactta acataggtcc tctttccata
     1021 tccatttgaa gaaaccgtaa gtatttcctc agtcggtttt tcagaaacaa aaacctggcc
     1081 gcaaaccact tcttcctttg gatttttgaa ccgtatccct attactccca tacctgtcct
     1141 accaattggt ttaacctcct gctcactgaa tcgtatgcaa aagccgtgct ttgtaaaaag
     1201 catgatgtcc ctgtcgccat cagtaagctt aacatcaatc agtttatcat cgttcttcag
     1261 tgtaattccg ataataccgc cctgtcttgg tttactgtat gcaataagag gcgttttctt
     1321 tactatacct ttttttgtca gcatcaaaat aaactgacct gaagaaaaat caggaaccgg
     1381 tatcactgcc gcgatttttt cctcagtttg aatcttcaaa agattcacaa ggggtttgcc
     1441 tcttgcaaca cgacttgctt caggaataag ataccccttc aaccagtgga ccctgcctgt
     1501 ttctgtaaat atcaacaatt gatcaatggt tgaacataca aatacatgtc tcacaaaatc
     1561 atcctctttt gtcccggcgc cgagaattcc acgcccaccg cgtcgctgtc ttctatacgt
     1621 ttcaaggggc aatctcttga tataaccgtg tgcagacaca gtgacaacaa tatcctcagg
     1681 acgtacaagg tcctcatcac tcaattcttc tattggagaa ccaatttctg tacgtctcct
     1741 gtcagaatat ttatttttca aaacctttaa ttctccttca ataatagcat taactttttc
     1801 tttgcttgca agaatagcct tcaattcagc aatttttttg ctaatctggt caagttcttc
     1861 ttctatcttg tttctctcaa gttttgttaa cttgcctaac ggcagagcga gaatcgcatc
     1921 agcctgtttc tgactcaaag aaaacttttt catcaggttt tctcttgctt cttcaacgct
     1981 gtttgaattt cttataagag aaatcacctt atcaagattg tcaacggcta ttttaagacc
     2041 caacaaaata tgatgtcttt cttctgcttt atttaattca aaacgagtcc gccggataac
     2101 aacatttctt ctgtgctcaa gaaataactc aagaatttct tttagagtaa gaagtttcgg
     2161 ctgtctgttt accagcgcaa ggttaataat tccgtacgtc gtctgtaatt gtgtatgtga
     2221 gtacaactga ttaataataa tatctgtgtt ttcacccttc cttgtctcaa gaacgatgcg
     2281 tataccttcc ttgcttgatt catccctgac attgctgacg ccacctattt tcttttcctc
     2341 aaccagtctg gcaatatttt caacaagtgt ggatttattc acctcataag gaatctcagt
     2401 aatcacgaca ttatcatgat tttttgcttc ttccacttcc acctttccgc gaattgttat
     2461 gatacctttt ccggtggtgt acgcttcaac aattccctga gttccacaga ttgttccacc
     2521 tgttgggaaa tcaggccctg taacaaattt catgatatcc tcaactgtac aatccgggtt
     2581 ctttatcatc ataatcagcg cgtcgcaaac ctcaccaagg ttgtgcggcg gaatattggt
     2641 tgccatacca actgcaattc cagaagagcc gttgattaat aaattcggga ctttgcttgg
     2701 aaggacaaca ggttcaacaa gggactcgtc aaaattaggc ataaaatcca ctgtgtcttt
     2761 ttcaatatcc gcaagtattt ccatagccag cggacgaagt cttgcttcag tatatctcat
     2821 tgccgctgga gggtcaccat caattgagcc aaagttcccc tgaccatcaa ccaggacata
     2881 tcttaatgtg aaatcctgag ccatccttgc aagggattcg taaataggcg aatcaccatg
     2941 aggatgatat ttacccatca cttctccaac gatacgagca cttttcttat aaggccttcc
     3001 tggttcaaga ccaagttcca tcatggcgta aagaattctt ctatgcacag gtttaagtcc
     3061 atccctaaca tcaggcaatg cgcgacctat gataacactc attgcgtaat caaggtagga
     3121 atttctcatt tcaatctcta tccctaccgg cgtaattttt tcctttggct gttgttgttt
     3181 ttctttatcg tccacaattc ctccttaaat atcaatgttt tttgcttcaa
//
LOCUS       DAORYU010000167         3227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_19862_length_3227_cov_7.214691, whole genome shotgun sequence.
ACCESSION   DAORYU010000167 DAORYU010000000
VERSION     DAORYU010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19862_length_3227_cov_7.214691"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="PLW07_05640"
     CDS             complement(<1..386)
                     /locus_tag="PLW07_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50043.1"
                     /translation="MNWKIELISGMILMCSVLSAVELKAQGLAAGKHFIFQTNGGETY
                     VMPPYKGATFKAIPIEDGITKIQVKYLSDNPKENRGPFSFTIDRKNLYWESGLDGGLF
                     FPAFLNHTIEVKSDKNIMIFLNFGGH"
     gene            complement(437..979)
                     /locus_tag="PLW07_05645"
     CDS             complement(437..979)
                     /locus_tag="PLW07_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50044.1"
                     /translation="MMVPGILDSHVHFNSIDKIKETKDFFTSCNIDRFCIVSPASPSG
                     GNSNPQALCFKATYPSETYIMGGLDYSNVMCYSTKERRKVLKPQLMRMLAIGFDGVKI
                     LETDDEFYMPEGDANVLSEKDRIIIGIKLPVSVLEKIYKKNFQHIVGKKPKPIDIEKA
                     IKESDRISGILTRQFNVRKN"
     gene            complement(991..1893)
                     /locus_tag="PLW07_05650"
     CDS             complement(991..1893)
                     /locus_tag="PLW07_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002646800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOL50045.1"
                     /translation="MERLVYINGQYYPEKEAKISIFDSSIMLGDTATESTRTFRHQPF
                     KLDKHIERLYQSLKLMHIHLELNPREMEKITLEVFEKNLPLLPEDEEYWIVHNISRGT
                     FLVGVAYKALCPTVMIFCLPLNQKEYAMYYKTGVHAIVPEIRQIPYSCIDPRIKHRSR
                     LHFVIADMEVRKIDPQGFCLLLDIDGNVTENKGANFFMVKKGVLITPSTKNCLAGISR
                     ATVLELSEELGIHSEERDITPEEICDADEAFFTSTPYCILPITKFNGRPVGEGTPGKI
                     TQLFLDAWSKKVGINIVEAALKKI"
     gene            complement(1929..2639)
                     /locus_tag="PLW07_05655"
     CDS             complement(1929..2639)
                     /locus_tag="PLW07_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOL50046.1"
                     /translation="MRKKGFTLIELLVVIAIIAILAAMLLPVLTRAREKARQAVCMNN
                     MKQVGLAMMLYAGDYDGWLASSIAGYTGWYGILFEKGYLKGKELVICPTDRIKTYDPY
                     AAGRTLAIDVYIGYYRDPAIYGWQFPCPTSTNKNVRRDAPGGPWYLRITHLPKPALFP
                     LIADASGCLPSNMGWPNAAYYIYDSNGAIVHLRHMGVANMWFADGHVEACNEDRVHYL
                     LPVYDILRYNLTVANYPP"
     gene            complement(2681..>3227)
                     /locus_tag="PLW07_05660"
     CDS             complement(2681..>3227)
                     /locus_tag="PLW07_05660"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOL50047.1"
                     /translation="KTPFINFVSTDHYKAGLTGAEYLVSRGHRSIVFVGLIKGNTCSY
                     HMYEGYRAALEKAGMRTSSPLIISACLNSKMKIGKGFHKKLNRVIEKHCPDGLFVAGE
                     LFLPDVLESLEKKNLIPGRDVDIVLSDEVPDGAPYKENIIGLIQPLSEIGDIAARCMK
                     QILNGEKKRIRVQIPPVLRKV"
BASE COUNT          889 a          732 c          556 g         1050 t
ORIGIN      
        1 taatgcccac caaaattcag aaaaatcata atgtttttgt cacttttcac ctcaattgtg
       61 tgattaagaa atgcagggaa aaaaagacct ccatccaggc cgctttccca gtaaagattt
      121 ttcctgtcaa ttgtaaaaga aaatggacct ctgttttctt ttggattgtc gctgagatac
      181 ttcacctgaa tttttgtaat accatcctca ataggtatcg ccttgaatgt tgctcccttg
      241 taaggaggca tcacataagt ttcgccacca tttgtttgaa aaataaagtg tttgcccgca
      301 gccagtccct gcgctttcag ttcaactgct gataatacac tgcacatcaa aatcatgcct
      361 gaaatcagtt ctattttcca gttcattctt ttcccccgat ttcttcaaga tattttttcg
      421 ccaggtaacc agaattttaa tttttcctga cattaaattg tctggtaagt attccgctga
      481 ttctatcgct ctcttttatc gccttttcaa tatcaatggg ttttggcttt ttccccacga
      541 tatgctgaaa attcttttta taaatttttt caagaacaga cactggcaat tttattccaa
      601 ttatgattct gtccttctct gaaagaacat tggcatcacc ttctggcatg taaaattcat
      661 cgtctgtttc aagaattttc acgccgtcaa atccaatcgc cagcattctc atcaattgcg
      721 gtttaagaac ttttctcctt tccttcgttg aataacacat aacatttgaa taatcaagcc
      781 ctcccatgat gtatgtttca gaaggatatg ttgccttgaa acagagcgcc tgcggatttg
      841 aattgccgcc tgatggactg gcaggactga ctatgcagaa tctgtcaata ttacaggatg
      901 tgaaaaaatc ctttgtttct ttgattttat caatgctgtt aaaatggaca tgagaatcca
      961 gaattccagg aaccatcata atgatccttt ttagattttt ttcaaagcag cttccacaat
     1021 gttaattcca acttttttgc tccatgcatc cagaaacaac tgtgtgattt ttcctggtgt
     1081 cccttcgccc actggtctgc cattgaattt tgttatcgga agaatgcaat aaggagtgct
     1141 tgtaaaaaac gcttcgtcag catcgcaaat ttcttcaggt gtgatatccc tttcttctga
     1201 atgtattccc agttcttcag aaagttcaag tactgttgcc ctgcttattc cagcaagaca
     1261 gtttttcgtg gaaggtgtta ttaacacccc cttttttacc ataaaaaaat ttgctccctt
     1321 gttttcagta acattgccat ctatatccag caggaggcaa aatccttgcg ggtcaatttt
     1381 tcttacctcc atatcagcga taacaaaatg aagtctgctt ctatgtttaa ttctcgggtc
     1441 aatgcaggaa tatggaatct gcctgatttc aggaacaatt gcatgaacac cagttttata
     1501 atacattgcg tattcttttt gattcaaagg aagacagaaa atcattactg tcggacacaa
     1561 tgccttatag gcaactccaa caagaaatgt cccacgggaa atgttatgaa caatccagta
     1621 ttcctcatct tcaggaagca aaggcaggtt tttctcaaac acctcaagag taattttttc
     1681 catctcccgc gggttcaatt caagatggat gtgcatcagt ttcaatgact gatacaatcg
     1741 ttcaatgtgt ttatccaatt tgaacggctg gtgtctgaaa gttcttgttg attctgtggc
     1801 agtatctccc agcatgatac tggagtcaaa gattgaaatc tttgcctctt tttccggata
     1861 atattgcccg ttaatgtaca ccagccgttc cacaggtctc ttcccttttt tctatgccac
     1921 ttcatcaatc atggaggata attagctact gtcagattgt atctcagaat atcgtaaaca
     1981 ggcagcagat aatgaaccct gtcctcatta catgcttcaa catgtccatc tgcaaaccac
     2041 atattggcaa cccccatgtg tctgagatga acgattgcac catttgaatc ataaatataa
     2101 tacgctgcat ttggccagcc catgtttgaa ggcaaacaac ctgatgcatc agcaatcaat
     2161 gggaaaagcg ctggttttgg aaggtgtgtg attctgagat accatggacc cccgggcgca
     2221 tcacgtctta cattcttatt tgttgatgtt ggacacggaa attgccaacc ataaatggct
     2281 gggtctctgt aataaccaat gtaaacatca attgcaaggg ttctacctgc tgcatatggg
     2341 tcgtatgttt ttattctatc tgtcggacat atcacaagtt ccttaccttt caggtatccc
     2401 ttttcaaaca gaattccata ccagcccgta taaccagcaa ttgatgaagc cagccatcca
     2461 tcatagtccc ctgcatatag catcatggca agtccaacct gtttcatatt gttcatacat
     2521 actgcctgcc tcgccttttc ccttgctctt gtcagcactg gcagtaacat cgctgcaagg
     2581 atggcgatga ttgctatcac aacaagaagt tcaatcaggg taaatccttt tttcctcatt
     2641 tttctcacct cctctccttt tttgaaaaat gttttgtacc ttatactttc cttaagacag
     2701 gtggtatctg aactctgatt cttttctttt ccccgttcaa aatctgtttc atgcaacgag
     2761 ctgcaatatc cccgatttca ctgagaggtt gaatgagacc aattatattt tctttataag
     2821 gagccccatc aggaacctcg tcagacagta caatatcaac atcccttcca ggaatcagat
     2881 ttttcttttc cagagattcc aatacatcag gcaaaaataa ttcaccggcg acaaacagcc
     2941 catccggaca gtgcttttca attaccctat tcaatttttt gtgaaaacct tttccgattt
     3001 tcattttact attgagacag gcagaaataa taaggggtga ggatgttctc atgcctgctt
     3061 tttcgagcgc ggcgcgataa ccttcataca tatgatatga acaggtattg cctttgataa
     3121 gtcccacaaa aacaattgac ctgtgccccc tggaaacaag gtattctgcg cctgtcaacc
     3181 ctgctttata atggtcagta cttacaaagt ttataaaagg agttttc
//
LOCUS       DAORYU010000168         3201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_20066_length_3201_cov_4.242530, whole genome shotgun sequence.
ACCESSION   DAORYU010000168 DAORYU010000000
VERSION     DAORYU010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20066_length_3201_cov_4.242530"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="PLW07_05665"
     CDS             <1..691
                     /locus_tag="PLW07_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50048.1"
                     /translation="HIINSGPGPAGQPIPSPFNAAGSSRKQFLVFRIDDKNLEMLCKD
                     ADGNILDRMVLKKQNGSYQKEVMDKAVDFELFKKVSNIYYELLGLGNYNLVVTTVSIP
                     QPGKKLILSLDLNKLPRGSLKKELLPEGIKLVVGQVKGSPWKLEKQEIPLTGDKVQFE
                     VIVPENFTMDQGIFKPDFEISINISLDGRLFEPFTTKVVFNPEIYQKISQSLDSNQQV
                     SSKFFILQHPS"
     gene            763..1281
                     /locus_tag="PLW07_05670"
     CDS             763..1281
                     /locus_tag="PLW07_05670"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOL50049.1"
                     /translation="MQKETDIEKALLRKYPEQVVLVTTRNKEGKNNVMAVGWTAIVSS
                     DPVMFVLGIDEEACTYQNILGTKQFVVSFPSEKMAKETLFVGSVHGYKRDKIQESGLE
                     IQEAKIVNAPVLVDAVANFECKLVEITKPGDCPLVVGQVVYAWENTDEHLKRLYTVGT
                     DHKLAGVRPIEP"
     gene            1285..>3201
                     /locus_tag="PLW07_05675"
     CDS             1285..>3201
                     /locus_tag="PLW07_05675"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOL50050.1"
                     /translation="MSRKILFIVFLVSLSALIFEISLMRVFSVLLRYHFVFLIVSMSM
                     CGLGCGGILWAFVQKKLKSHAKPGNTIFTLSILTGILMPVCIILLFKSPAKDFLTGYT
                     VISFIPFLPFLLAGCFFSYVFQHYGSSSGNIYFADLVGAGTGCLLVLWILNLGGINSC
                     FIASAICIVAAIIAGAGSMSRRKNLVSIFTLCLILFLIVANHRGKFFDIPYVSAQNNQ
                     IIKPLFTAIEQSNGSIVYTEWNAFARTDVVEFHQLQDIKYIYTDGDVPTTMHFFDGNV
                     SSIISALKNTVFYLPFADLKNPRVLSIGPGGGIDILVSILAGSKNITGVEVNPSMFSI
                     MERFAQFNGNLYQYPGVRVYLDDGRSFVKRTEEKYNVIYLALTQSATGQHTGGVLTEG
                     YIHTKEAFTDYLEKLEEDGMIVFVTQNELLLLRGFATFLQIFNKVHDPSALKRLMVFM
                     CPEEMFEATPYRYVLIAGKQNFSSQRLIKIKNLAENIGLIPVFVPGMVAKSPFDKFGD
                     FVDVETFVSTINSMLDQPVNLKPVSDDSPFFLDLSVGVSQQFKILLLIIVAVSIVCML
                     NVVLRNIEEIRNKNFTTGVFVLYFAIIGLAYMLVEIALIQKFILFLGHPTFAVSIVLF
                     GLLAASGTGSYVSQF"
BASE COUNT          960 a          552 c          642 g         1047 t
ORIGIN      
        1 tcacatcata aatagtggcc cgggtcctgc aggacaacca ataccaagtc catttaatgc
       61 tgccggttca tcccgaaaac aatttcttgt tttcagaata gatgacaaga atcttgaaat
      121 gctctgcaag gatgcagatg gaaatatcct tgacagaatg gttttgaaaa agcaaaatgg
      181 ttcatatcaa aaggaagtaa tggataaggc agttgatttt gaacttttca agaaagtttc
      241 taacatttac tatgaattac ttggtcttgg aaattacaat cttgtagtaa ctacagtttc
      301 aataccacaa ccagggaaaa aacttattct ttcgcttgac cttaataaac ttccacgtgg
      361 gtcactaaaa aaggaactgt tgcctgaagg gataaagctt gttgttgggc aagtcaaagg
      421 tagcccatgg aaacttgaaa aacaggaaat ccctctcact ggagataagg ttcaatttga
      481 ggtaattgta cctgaaaatt tcactatgga ccagggaata ttcaagccag attttgaaat
      541 ttcaataaat atttctcttg atggaagatt gtttgaacct tttaccacaa aggttgtttt
      601 caatcctgaa atatatcaaa aaatctctca atccctggat tcaaaccagc aggtatcatc
      661 gaaattcttt atccttcagc atccgtcctg agatagtaaa aacaataaaa tctgtgttaa
      721 gataataaag gtgatattat ctttaaaaat tcgggaggga atatgcagaa agaaacagat
      781 attgaaaagg cgctactgag aaaatatcca gaacaggtgg tacttgtcac aaccagaaat
      841 aaagaaggga aaaacaatgt gatggctgtg ggttggacag caattgtatc cagtgaccct
      901 gttatgtttg tgttaggtat agatgaagag gcatgcacct atcaaaacat tcttgggaca
      961 aaacagtttg tggtaagttt tccttcagaa aaaatggcaa aagaaaccct ttttgtcgga
     1021 agtgtacatg gatataaaag agacaagatc caggaatctg ggttggaaat tcaggaagca
     1081 aaaatcgtca atgcgccggt acttgtggat gcagtcgcta actttgagtg taaacttgtt
     1141 gaaattacaa aaccagggga ttgcccgctt gttgtgggac aggttgtgta tgcatgggaa
     1201 aatactgacg aacacttgaa acggctttat actgtgggca cagatcataa acttgctggc
     1261 gtaagaccga tagaaccata accaatgtca agaaaaatcc ttttcattgt ttttctcgtg
     1321 tccctttctg ctttgatatt tgaaatatcg ttgatgcgcg tgttttctgt ccttttaaga
     1381 tatcactttg tttttcttat cgtatctatg agcatgtgcg gacttggttg cggtggaata
     1441 ctgtgggcat ttgttcaaaa aaaattgaag tcacatgcca aaccaggaaa cacaattttt
     1501 actctctcca tacttactgg aattttgatg ccagtatgta tcatccttct tttcaaaagc
     1561 cctgccaaag attttcttac tggttatact gtcatttctt ttatcccgtt tcttccgttt
     1621 ttacttgcag gttgtttttt ttcatacgtt tttcaacact atggttcttc aagtggcaac
     1681 atttattttg ctgaccttgt tggtgctgga acaggatgtt tgctggtatt gtggatactg
     1741 aatcttggtg ggattaatag ttgttttata gcaagcgcaa tatgtattgt tgcagcaatc
     1801 attgcaggtg ctggctcaat gtcaagaaga aaaaaccttg tgtcaatttt tactctttgt
     1861 ttaattctat tccttatcgt tgcaaatcac agaggaaaat tttttgacat tccctatgta
     1921 tctgcacaaa acaatcaaat tataaaaccc ctttttactg ccattgaaca atcaaatggc
     1981 tcaattgttt atactgaatg gaatgccttt gcgagaacag atgttgtgga atttcatcaa
     2041 ttacaggata tcaaatatat ttacactgat ggtgatgtac caacaacaat gcattttttt
     2101 gatggcaatg tatcttctat tatcagtgct ttgaaaaata ctgtatttta tcttccgttt
     2161 gcggatttga aaaaccccag ggttttatcc attggtcctg gcggtggtat tgacattctt
     2221 gtttcaatac ttgcaggtag taagaatatt actggagttg aggttaatcc ttcgatgttt
     2281 tcaatcatgg aaaggtttgc gcaattcaat ggaaatcttt atcaatatcc tggtgtcagg
     2341 gtgtatcttg atgatggaag aagttttgtg aaaagaacag aagaaaaata caatgtgatt
     2401 tacctggctc tgacccagag cgcaacagga caacatacag gcggagttct cacagaggga
     2461 tacattcaca caaaagaggc attcacagat tatcttgaaa aacttgaaga agacggaatg
     2521 attgtatttg ttacccagaa tgaactgtta ctacttcgtg gctttgccac atttctccag
     2581 atatttaata aagtccacga tccttctgct cttaaacgcc tgatggtttt tatgtgccct
     2641 gaagaaatgt ttgaggcaac gccgtatcgg tatgtcctta ttgctggcaa gcaaaatttt
     2701 tccagccagc gactgataaa aatcaaaaat cttgctgaaa acatcgggct gattcctgtc
     2761 tttgttcctg gtatggttgc aaaaagtcct tttgataaat ttggagattt tgttgatgtg
     2821 gaaacatttg tcagtactat taattcaatg cttgaccagc cagtaaatct gaaacctgtt
     2881 tctgatgata gcccattttt tcttgactta agtgttggag tgtcacagca gtttaaaatc
     2941 cttttgctta ttattgtcgc tgtttccatt gtatgcatgc tgaatgttgt tttgagaaat
     3001 attgaagaga ttcgtaacaa gaatttcaca acaggtgtat ttgtactcta ttttgctatt
     3061 attggtcttg catatatgct tgttgaaata gcattgattc agaagtttat cctttttctg
     3121 ggacatccca catttgcagt gagtattgtt ctctttggac ttcttgctgc aagcggaaca
     3181 ggtagttatg tcagccagtt c
//
LOCUS       DAORYU010000169         3199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_20089_length_3199_cov_5.350509, whole genome shotgun sequence.
ACCESSION   DAORYU010000169 DAORYU010000000
VERSION     DAORYU010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20089_length_3199_cov_5.350509"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1358)
                     /locus_tag="PLW07_05680"
     CDS             complement(<1..1358)
                     /locus_tag="PLW07_05680"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOL50051.1"
                     /translation="MKVKVLFSVLVMAVAGLSFADMMIVEKGVPKTTIVVSKSAIGVP
                     TEPTEQTFWYPQPAPNKIAAAAKDLQQYIEKMTGAKLPVVSDDQQVTGNIICVGRSNY
                     TKQFDKKIPSGLTPTRDEEGFVIICQGNTLVLAGNDEPYYHGTEYAVANFLHRIGVRW
                     YMPGEYGEFVPKKDTITVSNMNILERPDFKMRNWWGPLASDLRIQEYRWKIRNMMNPI
                     LDFVTLPRDSSVRSVLPPASEVNNPEYAEIFGKDEKGNPNPGMPNVTSEKSVKYAAEK
                     IKEYFRKNPGITSYGIGADDGYPRDYSPGTLKRNLGLPDTGGRAGVPSDMSTTEEWME
                     WIQAVAKEVYKEFPDHIITTNGYANRNVPPQGITPDPKIWIMFAAIWCDTIHAYDNPL
                     SWQTLRQAEMIRQWAFMYKNVYMYNYMYYMLAGCGAPIPLAHKHMHDMPLYKKWGVVG
                     FS"
     gene            complement(1355..2839)
                     /locus_tag="PLW07_05685"
     CDS             complement(1355..2839)
                     /locus_tag="PLW07_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50052.1"
                     /translation="MEKKNFALIDERLVEDTLYLKRALEIPEFPHKKPVVSPGEGIGS
                     VLRDKNGLWKMWYTVFITRDPQKDMVGCETPMVLAYSHDDIAKDPVYKDHPNIVVHAR
                     MKDASGRYISGWGGPAGMCILDAEMTPHPAARKRFTGLYVDFPIDVIGGICIADSDDG
                     IHWTIWPESPVIIGSTDTQNILLYDKNIKKYVCYLRPVIHCGMDRHANRKIARCESED
                     LIHWTIPEVVLDTDELDAPGLEVFDEPGMRGARGRNKQFQGISPFIRNNCYIAFTWFY
                     DAVKGIFTNELIHSSDGINWKREPLRESFIADNKPEGFRGKLPVPFGTAPIDVGDTMY
                     FYSSNIPYGHHEIAVADINGSVKNRQQLLESNEIYLITLKRDRWIYYQAGEIEGELLT
                     SPAEWEGGGSLCLNAKIEKDGYIRVEVDDMYGRKINDAHLDEINLITGPVNSVDIPVT
                     FGPGPKTVMKFPLLGPIRFRFWMKKAKLYGWSFIHPVYPQKEER"
     gene            complement(2841..3011)
                     /locus_tag="PLW07_05690"
     CDS             complement(2841..3011)
                     /locus_tag="PLW07_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50053.1"
                     /translation="MLANFLPEPVRARNVEFKQTGSNKCLIYKVFEQPVETKFPVSLS
                     VNGERVVFIVEG"
BASE COUNT          861 a          707 c          548 g         1083 t
ORIGIN      
        1 tttgaaaatc caacaactcc ccatttttta tacagtggca tgtcatgcat gtgtttgtgg
       61 gcaagcggaa ttggggcacc acaaccagca agcatatagt acatgtaatt gtacatatac
      121 acatttttgt acatgaatgc ccactgtcgt atcatttctg cctgtctcag tgtttgccat
      181 gacagagggt tgtcgtatgc atgaattgtg tcgcaccata ttgctgcaaa cataatccag
      241 atttttgggt caggtgtgat accctgtggt gggacatttc ggtttgcata accgtttgta
      301 gtaatgatat ggtcaggaaa ctctttataa acttctttcg caactgcctg aatccattcc
      361 atccattctt ctgtggtgct catgtctgat ggaacaccag cccttccgcc agtatccggc
      421 aatcccaaat ttcttttcaa cgttccaggg ctataatccc tgggatatcc atcatcagcg
      481 ccaattccat aggaagtaat accaggattt ttcctgaagt attccttgat tttttcagca
      541 gcgtatttta ctgatttttc gcttgttacg tttggcattc caggatttgg atttcctttt
      601 tcatcctttc caaaaatttc tgcgtattct ggattgttca cttctgatgc aggtggaagc
      661 acgcttctta ctgaactatc tcgtggtagt gtcacaaaat ctaaaattgg attcatcata
      721 tttcgtatct tccatctgta ttcctgaatt cttaaatcag aagcaagtgg tccccaccag
      781 tttctcatct tgaagtcagg tctttcgaga atattcatat ttgatacagt gattgtgtcc
      841 ttttttggaa caaattcacc gtattcacca ggcatgtacc agcgtactcc aatcctgtga
      901 agaaagtttg ccacagcata ttcagttccg tgataatacg gttcgtcatt accagcaaga
      961 acaagggtat ttccttgaca tataatgaca aatccttctt catctcttgt tggagttaga
     1021 cccgaaggaa tttttttgtc aaattgtttt gtgtaattac ttcttcctac gcaaatgata
     1081 ttgcctgtga cctgctggtc atcagaaaca actggaagtt ttgctcctgt cattttttca
     1141 atatattgtt gcaggtcctt tgctgctgca gcaattttgt ttggcgcagg ttgaggatac
     1201 cagaatgttt gttcagttgg ttctgttggt acgccaattg cactttttga aaccacaatc
     1261 gttgttttcg gcacaccttt ttctacaatc atcatatcag caaaacttaa acctgccact
     1321 gccattacca gcactgaaaa taatactttt actttcatct ttcctccttc tgtgggtaaa
     1381 caggatggat aaaactccat ccataaagtt ttgccttctt catccagaac ctgaaccgga
     1441 ttggtccgag aagtggaaat ttcatcactg tttttggacc aggtccaaaa gttacaggta
     1501 tatctacact gttaacagga cctgtaataa gattgatttc atcaagatgc gcatcattaa
     1561 ttttccttcc atacatatcg tcaacttcaa ccctgatgta accatctttt tcaatctttg
     1621 cattgagaca caaactacca ccaccttccc attctgctgg acttgttagt aattctcctt
     1681 caatttctcc tgcctgataa taaatccatc gatccctttt caatgttatc aaataaatct
     1741 catttgattc aagaagttgt tgtctgttct tcacacttcc attgatgtcc gcaacagcaa
     1801 tttcatgatg tccataggga atgttgcttg agtaaaaata catggtatct ccaacatcaa
     1861 tcggtgcagt tccaaagggt acaggaagtt ttcccctgaa tccttctggt ttattgtctg
     1921 caatgaatga ttctcttaat ggttcccttt tccagttaat tccgtcactg ctgtgaatca
     1981 gttcatttgt aaaaattcct tttactgcat cataaaacca tgtaaacgca atgtaacagt
     2041 tgtttctgat aaatggagaa attccctgaa actgtttgtt tcttcctctt gcgccacgca
     2101 ttcctggctc atcaaaaact tcaagtcctg gtgcatcaag ttcatcagtg tcaagaacca
     2161 cttctggaat tgtccagtga attaaatctt cactttcgca tcttgcaatt tttctgtttg
     2221 catgcctgtc cataccacag tgaatgacag gtctcaaata acagacatat ttttttatgt
     2281 ttttatcata aagcaggatg ttctgtgtgt cagttgaacc aataataaca ggactttcag
     2341 gccatatcgt ccagtgtata ccgtcatcgc tgtcagctat gcagatacca ccaatgacat
     2401 caatgggaaa atcaacatac agtcctgtaa atctttttct tgctgccgga tgtggagtca
     2461 tttcagcatc caggatgcac attccagccg ggccacccca acctgaaata tatcttccac
     2521 tggcgtcctt cattcttgca tgaacaacaa tattgggatg gtctttataa accgggtctt
     2581 ttgcaatgtc atcgtgtgaa taagcaagta ccatgggtgt ttcgcatccc accatatctt
     2641 tctgagggtc tcttgtgata aaaactgtgt accacatttt ccagagtcca tttttatccc
     2701 ttaatacaga accaattcct tctccgggcg aaacaactgg ttttttatga ggaaattctg
     2761 gtatttcaag cgctcttttg agatacaggg tatcttcaac gagtcgttca tcaatcaatg
     2821 caaaattttt cttttccata tcatccctcc acaataaaaa ccacccgttc accattcaca
     2881 ctcaatgaca ctggaaactt tgtttcaaca ggttgttcaa atactttgta gataagacat
     2941 ttgtttgaac cggtttgttt aaattctaca tttctggctc tgaccggttc aggaagaaaa
     3001 tttgccaaca tcaggaaatt ttctttttat ccctatgaaa ttggaccgat gttcaatgac
     3061 acaaaaatct aaatcgattg atatagatag tcaaaaattg tcaaaaacaa attgttatta
     3121 aaaattatac ctgtttcagg tattctatcc cacttcattt cattgtcaag catgcgcaga
     3181 tgaaaatttg acttgattt
//
LOCUS       DAORYU010000170         3197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_20109_length_3197_cov_4.905474, whole genome shotgun sequence.
ACCESSION   DAORYU010000170 DAORYU010000000
VERSION     DAORYU010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20109_length_3197_cov_4.905474"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            34..1167
                     /locus_tag="PLW07_05695"
     CDS             34..1167
                     /locus_tag="PLW07_05695"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOL50054.1"
                     /translation="MIKVSSDIPYGNVCDVSIETNRDSATVYFASSPKEGSEALWFCF
                     RITTTKKLKSIQLVLKHPYNMLMGSCDLSNILPVVKKDDSTWKRIEKTYQFHTKQDGR
                     YQVFWILNNPGKINDIAFCYPYGIQEVENLVKGTTGYLKKDTIGVSSENKPIVRLSND
                     YSEPGSKKAGIFIVARQHSGETPGSWVLEGILRYLAANRIKDIVLWAIPLSNIDGIEK
                     GCYGKNYFPHDLNRSWGKLPLRHEILVIQRDVARWQERCSPILGIDLHAPGGAEGNGV
                     YFPFYKEQSPVKKMEKYWIDVIGRTIGDEYMFPEYKKHSKPEGDLKNLTGLMFSTYFS
                     ICLKIPGLCLEIPYGINKGKILTIKDYMDIGKRMIRGILEAIK"
     gene            1164..2012
                     /locus_tag="PLW07_05700"
     CDS             1164..2012
                     /locus_tag="PLW07_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50055.1"
                     /translation="MNQEKIHLPKIDFCGHKISKIILGSNPFSGFSHQTKELDEEMLD
                     FYTSEKIKKAWDQAQIYGINGFCARGDRHIVRILREYYNEGRAKNFYWFAQTAPEFSS
                     FQANLSLITGSKLKPAFIYHHGGQLDLLLMEGKKQQVKDHLKMIRDTGYPVGLASHQP
                     EFIIMAEEENWDIDFYLCCFFNLTGRGKNELTAIKGSEGEIFDMEDPPKMCQVIKQVK
                     KPCIAYKIYGAGRLCNDRNDLCSALKFANENIKQSDLILIGVFQKYKNQIKENVEMFY
                     KIITTS"
     gene            2058..>3197
                     /locus_tag="PLW07_05705"
     CDS             2058..>3197
                     /locus_tag="PLW07_05705"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOL50056.1"
                     /translation="MNRKILSLISTTCIFASVAFRKNVSDEVQNIVNSNNKFAVNFYN
                     ELAKTGNNNLFFSPFSIIDAFAMAYEGATGKTANEIKSTLFLPAKDNQRLSGFQLLHN
                     QINQKKSDCILSIANAYWVQHNFVLKKKYLETIEKFYQSEAYNLDFKMNSQQAVDRIN
                     NWAEEKTKGKIKKFLNYGDVDSGTRLVLTNAIYFKALWQIQFNKNLTEPADFRVKENK
                     TIKVQMMHLQNAEFPYAETEDFQLLKIPYGCNNLSILIILPRTLNEEMVRKIDYEKIM
                     EIQKNLSKQKIDISIPKVKIEQTYDLINPLRNLGIKTAFDPFAADFSKITGSRDLYIS
                     KAVQKSFIEINEEGTEAAAATGIVMNITAARPGVKKVFVADHPFIF"
BASE COUNT         1201 a          542 c          588 g          866 t
ORIGIN      
        1 aagaaaaaac ttataagaat tacaatttaa gaaatgataa aggtctcatc tgatatcccg
       61 tatggcaatg tctgtgatgt ttcaattgaa acgaacaggg acagtgcgac tgtttatttt
      121 gcatcatcac caaaagaagg ttctgaggca ctctggtttt gtttcagaat tactacaaca
      181 aaaaaattaa aatcaataca acttgtttta aaacatcctt ataacatgct tatgggaagc
      241 tgtgaccttt caaatatctt accggttgta aaaaaagatg attcaacatg gaaaagaata
      301 gaaaaaacat accagtttca tacaaaacag gatggaagat atcaggtatt ctggatatta
      361 aataatccag gaaaaattaa tgacattgcg ttctgttatc catatggaat acaggaagta
      421 gaaaatcttg taaaaggaac aacgggctac ttaaaaaaag acaccatcgg tgtcagttct
      481 gaaaacaaac ccattgtaag gttgtccaat gattactctg aacctggttc aaaaaaggca
      541 ggaattttca ttgtagcaag acagcattca ggtgaaacac caggaagctg ggtccttgaa
      601 ggcattttaa gatacctcgc agcaaataga ataaaagata ttgttttatg ggcaatcccg
      661 ctttcaaaca ttgacggaat tgaaaaaggt tgctacggca agaactattt tccacatgat
      721 ttgaaccgct catggggaaa acttccactc agacatgaaa ttttggtcat ccaaagagac
      781 gtagcaagat ggcaggaaag atgttctcca atattaggaa tagaccttca tgcgcctggt
      841 ggggctgaag gaaacggagt atatttccca ttttataaag aacagtcacc tgtgaaaaaa
      901 atggaaaaat actggattga tgttattggc agaaccatcg gtgatgaata tatgttccct
      961 gaatacaaaa agcattcaaa gccagaggga gatttgaaaa atctcactgg tctgatgttc
     1021 agtacttatt tcagcatctg tctgaaaatt cctggactct gccttgaaat tccttatggc
     1081 attaacaaag gaaagatact aacaataaag gactacatgg acattgggaa aagaatgatt
     1141 cgcggcattc tggaagcaat taaatgaatc aggaaaaaat tcatttacca aagatagatt
     1201 tttgtggcca taaaatctca aaaattatcc ttggctcaaa tccattcagt gggttttctc
     1261 accaaacaaa agaattagat gaagaaatgc ttgattttta tacgtcagaa aaaataaaaa
     1321 aagcgtggga ccaagcacaa atatatggaa tcaatgggtt ctgtgcccgc ggagatagac
     1381 atattgtgag aatcctcagg gaatattata atgaaggcag ggcgaaaaat ttttactggt
     1441 ttgctcagac agcgcctgag ttttcaagtt ttcaggcaaa cctatctctt attactgggt
     1501 caaaattgaa acccgccttt atatatcatc atggtggtca actcgacctt cttttaatgg
     1561 aagggaaaaa acagcaagta aaggaccact taaaaatgat aagagataca ggatatccag
     1621 taggacttgc ttcacaccaa cctgaattta tcattatggc tgaagaagaa aactgggaca
     1681 ttgatttcta cctgtgctgc ttttttaatc ttacaggacg gggaaaaaac gaactcacag
     1741 caataaaagg ttctgaagga gaaatatttg acatggagga cccaccaaaa atgtgtcagg
     1801 taataaaaca ggtgaaaaaa ccctgtatcg catacaaaat ttatggtgct ggaaggttat
     1861 gtaatgacag aaatgacctc tgctctgcat tgaaatttgc aaatgaaaac ataaaacaaa
     1921 gcgatttaat tctcattggc gtttttcaaa aatacaagaa tcagataaaa gaaaacgtgg
     1981 aaatgtttta taaaatcatt acaacttcct gaacttttcg tccttttgtc ctgtctaatt
     2041 tttaacagga gatgcccatg aataggaaaa ttcttagtct aatttcaacc acgtgtatat
     2101 ttgcctcagt cgcttttcgt aaaaatgtct cagatgaagt tcaaaatatt gtcaattcaa
     2161 acaataaatt tgcggtaaat ttctataacg aacttgcaaa gacaggaaat aacaatcttt
     2221 tcttttcacc tttcagtatt attgatgcat tcgcaatggc atatgagggt gccacaggaa
     2281 aaactgcaaa tgaaatcaaa tccactttat ttctaccagc aaaggataat caacgcctgt
     2341 ctggatttca gttactgcat aaccaaatta accagaaaaa atcagattgc atattgtcta
     2401 tcgcgaacgc atactgggta cagcataact ttgttctgaa gaaaaagtat ttagaaacaa
     2461 ttgaaaaatt ttatcaaagt gaagcctaca atcttgattt caaaatgaac tcacaacagg
     2521 ctgttgacag aattaacaac tgggcagaag aaaaaaccaa aggaaaaatc aaaaaatttt
     2581 taaactatgg cgatgttgac tcaggaacgc ggcttgtatt gacaaatgcc atttatttta
     2641 aggcactgtg gcaaattcag tttaataaaa atctaacaga accagctgac tttagggtca
     2701 aggaaaacaa gacaataaaa gtgcagatga tgcatctgca gaacgcagaa tttccctatg
     2761 cagaaacaga ggattttcaa cttctcaaaa ttccatatgg atgtaataat ctttcaattt
     2821 tgataattct gccgagaaca ttaaacgaag aaatggtcag aaaaatagat tatgaaaaga
     2881 ttatggaaat ccaaaaaaat ctttcaaaac agaagataga catttccatt ccgaaagtca
     2941 aaattgaaca aacatatgat ttaataaacc cattaagaaa tcttggaata aaaactgcct
     3001 ttgatccgtt tgcagctgat ttttcaaaaa taacaggaag cagagaccta tatatttcaa
     3061 aagcagtcca aaaatccttt attgaaatca acgaagaagg aacagaagca gcggctgcta
     3121 caggaattgt aatgaacatc acagcagcaa gacccggagt aaaaaaagta tttgtagcag
     3181 accatccatt catattt
//
LOCUS       DAORYU010000171         3179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_20255_length_3179_cov_4.759603, whole genome shotgun sequence.
ACCESSION   DAORYU010000171 DAORYU010000000
VERSION     DAORYU010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20255_length_3179_cov_4.759603"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1085
                     /locus_tag="PLW07_05710"
     CDS             <1..1085
                     /locus_tag="PLW07_05710"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HOL50057.1"
                     /translation="KDLVTLVERLKKEEEIAFDIEGNSLYSYHSKICLIQVSTRSENF
                     IIDPLKIKNTYVLNEVFTNPHIVKVLHGSVYDVQMLYQHSGIIINNLFDTQIAAHFLG
                     EKKTGLANLLEKHFHVHLDKRHQKSNWGIRPLTPEMLDYATSDTKYLLPLADRLREDL
                     NKMHRTEWVIEECEALARSVLNGKPAKTKIIRGAGKIPKKKMAIVEAIVEFRDIIAKE
                     KDIPPFRVLDKDTIFKIATTEHIDLKKLQSITSNHRHIQKHLEELLERIKKCPARQYK
                     ATYHKKVHHPFFSQKAEKLYEWRQSKAENLGIQCHLVLSHHQIVTLASSNIRSLEDME
                     KTECLKRWQKQEFGNEIFSLLRRKGI"
     gene            complement(1104..1682)
                     /locus_tag="PLW07_05715"
     CDS             complement(1104..1682)
                     /locus_tag="PLW07_05715"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOL50058.1"
                     /translation="MILWKRKLYRIILGGLVPFVYLLTKDPLAPALIASFFLFWLLLM
                     EFERYLHPGFWNWLLKHSPGIFKTRPGVLTGDTNFMIASFISVLYFPLPVAVANLLFL
                     TFGDAASAMIGTKYGKLHIFPGKTVEGMLGGIIINCIIGVLVSFYFAIPFEILFAGVL
                     TGCILEVLPLKIDDNLTVGIIPGILMTILLNG"
     gene            complement(1679..2254)
                     /locus_tag="PLW07_05720"
     CDS             complement(1679..2254)
                     /locus_tag="PLW07_05720"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HOL50059.1"
                     /translation="MELILLVIAGYITGSISFAYLFTFFLTGKDIRTIGTKNPGAANV
                     AREIGKKWGIIVWLADTIKGAGIMAVASFSGITNEIFLTIIGISAVIGHCYPVFLKFK
                     GGKGIATMGGVMLYLAPLVFPFIIGFWFIAQAINPRSLKIILPEVILFFVILWQIYPH
                     AFTNLAISTLLLIITNAFINREALKEMRRAH"
     gene            2254..2934
                     /locus_tag="PLW07_05725"
     CDS             2254..2934
                     /locus_tag="PLW07_05725"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOL50060.1"
                     /translation="MKKLYLRLFILTRLSLCYNKGVEYRTAINSDLPECARLYIDGFK
                     HQLHTIFGRHIPEKMFIDFLSYVRKLEKNGFIVACENGKIAGFTIMSVNPLNIYLNVF
                     FLSFFPSLFNFISGKYSGICFHKVLTSLFDFVKFFRKSSIADRKYHDAGQVITMVVCE
                     SMRGRGIGGNLLKNGLDYLKKHTDTVKLEVRQDNLPAIQLYKKNQFVEIGEVHSKVGT
                     SLVMVRHL"
     gene            complement(2989..>3179)
                     /locus_tag="PLW07_05730"
     CDS             complement(2989..>3179)
                     /locus_tag="PLW07_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50061.1"
                     /translation="ENERRKKRGWGRFQKIPLSPPFVKGEIMRRNLKGGRMRGGKRGA
                     GGDFKKSPSIPLLKRGKL"
BASE COUNT         1090 a          651 c          565 g          873 t
ORIGIN      
        1 aaaaagacct ggtgacattg gtagaaagat tgaaaaaaga agaagaaatt gcttttgaca
       61 ttgagggtaa ttctctttac agttatcact caaaaatttg cctgatacag gtttcaacaa
      121 gaagcgaaaa tttcatcatt gaccctctga aaataaaaaa tacttatgta ttgaacgaag
      181 tattcaccaa cccccatatt gtaaaggtgc ttcatggttc tgtttatgac gtacagatgc
      241 tctaccagca ctcaggcatc ataataaata atctttttga cacccagatt gcagcccact
      301 ttctcggaga aaaaaagaca ggccttgcca atctgcttga gaaacacttt catgtccatc
      361 ttgacaaaag acaccagaaa agcaactggg gaataagacc actgactcct gaaatgcttg
      421 attacgcaac cagtgacaca aagtatcttt tgcctcttgc tgacaggtta agagaagatt
      481 taaacaaaat gcacagaaca gaatgggtca ttgaagaatg cgaagcactt gcccggtcag
      541 tactcaatgg aaaaccagca aagacaaaaa ttatcagggg agcagggaaa attccaaaaa
      601 aaaagatggc aatcgtggaa gcaatcgttg aattcagaga tattattgca aaggaaaaag
      661 acattccacc cttcagggtt cttgataaag acaccatatt caaaattgcc acaaccgaac
      721 acattgacct taaaaaactt caatccatca catcaaatca cagacatatt cagaaacatc
      781 ttgaagaatt acttgagaga ataaaaaaat gccctgccag acaatacaaa gctacctacc
      841 ataaaaaagt acatcatcct ttcttttccc agaaagcgga aaaactatac gagtggaggc
      901 agtcaaaagc agaaaatctg ggaatacaat gccatcttgt gttatcccat catcagattg
      961 ttactcttgc ttcgtcaaat atacgttcat tggaggacat ggaaaaaaca gaatgtctga
     1021 aaagatggca gaaacaagaa tttggaaacg aaatattttc cttgttgcga agaaaaggca
     1081 tataaacatc tcatcagtat tttttaacca ttaagcaaga ttgtcatcaa aatgcctgga
     1141 attattccta ctgtgaggtt gtcatcaatt tttaatggta atacttcaag gatgcaacca
     1201 gtcagcactc cagcaaataa aatttcaaat ggtatcgcga agtaaaaaga aacaagcact
     1261 ccaataatac aattgataat aattccacca agcatgcctt caacagtttt tccaggaaaa
     1321 atgtggagtt ttccatattt tgtgccaatc attgcacttg cggcatcacc aaaagtgaga
     1381 aaaagtaaat tcgcaacagc aacaggtaat ggaaaatata aaacagaaat aaacgacgct
     1441 atcatgaaat ttgtatcacc tgtaagaacc cctggccttg ttttgaatat acctggagag
     1501 tgtttcaaaa gccagttcca gaagcctgga tgaagatacc tttcaaattc catcaacagt
     1561 aaccagaata aaaaaaacga agcaatcaaa gcaggcgcaa gagggtcttt tgtcaaaaga
     1621 tacacaaagg gaacaagtcc tcccagaata attctgtata attttctttt ccagagaatc
     1681 aatgggctct cctcatttcc tttaatgctt cccgattgat aaaggcattg gtaataatca
     1741 aaagcaatgt tgaaattgca aggtttgtga aagcgtgcgg gtaaatctgc cagagtatta
     1801 caaagaacaa tattacctca ggaagaatga ttttcagtga acgcgggtta attgcctggg
     1861 caatgaacca gaatccaatg atgaaaggaa acacaagtgg cgcaaggtat agcatcaccc
     1921 cacccattgt agcaatgccc tttccacctt tgaatttcag aaaaactgga tagcaatgac
     1981 cgatgacagc agaaatgcca ataattgtca gaaagatttc atttgtaatg ccagaaaaac
     2041 tggcaacagc cataatccct gcgcccttga ttgtatcagc aagccagaca ataatccccc
     2101 actttttgcc aatttccctt gcaacatttg cagccccagg gttttttgtg ccaattgtgc
     2161 ggatgtcctt acctgtcaga aaaaaagtga ataggtatgc aaaagaaata cttcctgtga
     2221 tatagccagc aatgacaaga agaattaatt ccattgaaaa aattatacct gcgacttttc
     2281 attctgacaa ggttgtctct gtgttacaat aaaggtgtgg aatacagaac agcgataaat
     2341 tctgatttgc ctgaatgcgc acgattatac attgatgggt tcaaacatca attacacaca
     2401 atctttggca ggcatatccc tgaaaaaatg tttattgatt ttctttcata cgtacgaaaa
     2461 cttgaaaaaa atggatttat cgttgcatgt gaaaatggga aaatcgctgg ttttaccatc
     2521 atgagcgtaa atcccttaaa catctatttg aatgtttttt ttctgagttt ttttccttca
     2581 ctgttcaatt ttatttcagg taaatacagt gggatttgct tccacaaagt actcacatca
     2641 ttgtttgatt ttgtgaagtt tttccggaaa agtagtattg cagacagaaa ataccatgat
     2701 gcaggacagg tcatcacaat ggtcgtatgt gaaagtatgc gcggtcgtgg tattggagga
     2761 aatctgttaa aaaacggtct tgattatcta aaaaaacata cagacactgt aaagctggaa
     2821 gtcaggcagg acaatcttcc agcaatccaa ctttacaaaa aaaaccagtt tgtggaaata
     2881 ggtgaagttc actcaaaggt gggtacttcc cttgtcatgg tcagacattt ataatacttt
     2941 cccccttttc aaatcccttc ttctattctc cccctttcaa atttctcctc ataatttccc
     3001 ccttttcaaa agggggattg agggggattt tttgaaatct cccccagccc ctctttttcc
     3061 tcctctcatt ctcccccctt tcaaatttct cctcataatt tcccccttta caaaaggggg
     3121 actaaggggg attttttgaa atctccccca gcccctcttt ttcctcctct cattctccc
//
LOCUS       DAORYU010000172         3171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_20325_length_3171_cov_4.575738, whole genome shotgun sequence.
ACCESSION   DAORYU010000172 DAORYU010000000
VERSION     DAORYU010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20325_length_3171_cov_4.575738"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /gene="fusA"
                     /locus_tag="PLW07_05735"
     CDS             <1..815
                     /gene="fusA"
                     /locus_tag="PLW07_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872259.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOL50062.1"
                     /translation="ISKIMDEDPTIKVFRDTETNETIISGMGDVHLDVVIGKFKEKFG
                     VEVVRGTPKIAYKETITTTASAEGKHKKQTGGRGQYGHCFLRVEPLPRGTGFEFVDEI
                     FGGAIPRQYVPSVEKGVREAMSRGVLAGYPIVDVKVTVYDGSYHVVDSSDIAFQLAGI
                     LALQKAVQDARPVLLEPIMNVEVSVPQEFVGDVMGTINAKRGRVLEMTTHGNSQVIRA
                     QVPLAEMSNYATELRSLTSGRGTFAMSFSHYEEVPPHLAEKIIEAKKSKSEA"
     gene            817..1359
                     /locus_tag="PLW07_05740"
     CDS             817..1359
                     /locus_tag="PLW07_05740"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOL50063.1"
                     /translation="MVRVVVENIVLNIATSTPIVILKDTETGGILPIVIGLFEAQAIL
                     LILEKEEFSRPLTHDLLKSVIEHMNGKIMKLEIHSLRENTYYANLIIESDKGQIKVDC
                     RPSDGIAIALRASAPIFASENLIESPDVIKYYTGKESLKAAPSDRPINQKEAEEFRKY
                     LEHLNAQDFWKKLKEETGEP"
     gene            1375..2124
                     /locus_tag="PLW07_05745"
     CDS             1375..2124
                     /locus_tag="PLW07_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOL50064.1"
                     /translation="MSGHNKWASIKHKKMATDARRGKMFTKIIREIMMAAKLGGGNPD
                     SNPHLRMAIERAKNINMPNDNIQRAIKKGTGEEGGVMLESVGYEGYGPGGVAILVETL
                     TDNKRRTAAEIRSIFSRHSGNLGESGCVSWMFERKGVVTLKNKEIDEEKLLSLVLDAG
                     AEDLKLDNDVFEIVCAPDKLESVKKILEENKISTESASVNLIPKNTVRVEGKEAEQLL
                     KLLDELEEHDDVQNVHSNFDIPDALIEAMSR"
     gene            2127..2630
                     /locus_tag="PLW07_05750"
     CDS             2127..2630
                     /locus_tag="PLW07_05750"
                     /inference="COORDINATES: protein motif:HMM:NF014166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOL50065.1"
                     /translation="MNNSITVMGVDPGLNSFGYGIISEDTRALHPLKYGQHSVPKGVS
                     FNDKLVYIGDTFSSIIDEIRPDFIAIEKIYVAKNTQIALSIGIVSGIIAHTGISKGVQ
                     VAFITPGEIKKIITGSGQAIKQQVCFMVQKLLGLKEDPGEHAIDALATAICFISINRF
                     NNLVGKL"
     gene            2627..>3171
                     /gene="ruvA"
                     /locus_tag="PLW07_05755"
     CDS             2627..>3171
                     /gene="ruvA"
                     /locus_tag="PLW07_05755"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOL50066.1"
                     /translation="MICSVEGKLVNKTPMEIVVDVSGIGYVIKVPLSTSSVLPDQGSV
                     VRLFTTLMIREENIELYGFATEKEWKIFQKMILLPGVGPKMALRILSRLSPDEILNAV
                     ISQDIDFLKRTPGIGKKTAERLVMELKHHIEGIEFVSEELSGDFIPEAIEALTVLGYK
                     RNDAQQIVRKTAKEIEKKPESL"
BASE COUNT         1024 a          512 c          758 g          877 t
ORIGIN      
        1 cgatttcaaa aattatggat gaagacccaa ccatcaaggt tttcagagat acagaaacta
       61 atgaaaccat tatttctgga atgggtgatg tacatcttga tgttgttatt ggtaagttca
      121 aggaaaagtt cggcgttgag gtggtaagag ggacaccaaa aattgcctac aaggagacaa
      181 ttacaacgac ggcttcagca gagggcaagc acaaaaaaca gacaggtggc agagggcaat
      241 atgggcattg tttcttgagg gttgaacctt tgcccagggg tacgggtttt gagtttgtcg
      301 atgagatttt tggtggagcg attccacggc agtatgtacc gtctgttgaa aaaggtgtca
      361 gggaggcaat gtcaaggggt gtgcttgctg gctatccgat tgttgatgtt aaggttacag
      421 tgtatgatgg ttcttatcat gttgtggatt catctgatat tgcgtttcaa ctcgccggga
      481 tactggctct tcaaaaagcg gttcaggacg caagaccagt tcttcttgaa cccatcatga
      541 atgtggaggt ttctgttccc caggaatttg ttggggatgt tatgggaaca atcaatgcca
      601 aaagaggtag ggtacttgag atgacgacac atggaaatag tcaggtaatc agagcccagg
      661 ttcctcttgc tgaaatgtcc aattatgcaa ctgagttaag gtctttgaca agcggaagag
      721 gaacttttgc aatgagtttt tctcactatg aagaggttcc gcctcattta gcagagaaaa
      781 ttattgaggc aaaaaaatca aaatcagagg cctgatatgg tgagggtagt tgtggaaaac
      841 attgttctta atatcgcaac aagcacgccg attgtgattc ttaaggacac agagacagga
      901 ggtattcttc ctattgttat cggtttattt gaagcacagg caattctttt gatacttgaa
      961 aaagaggaat tttcaaggcc tctgactcac gatcttctga aatcagtgat agaacacatg
     1021 aatggaaaaa ttatgaaact tgaaatacat tctttgagag aaaacacata ttatgccaac
     1081 cttatcattg aatcagacaa agggcagata aaggtggatt gcaggccgag cgatggtatt
     1141 gctattgctt taagggcatc tgcgccgata tttgcttcag agaatttgat tgaatcgcct
     1201 gatgttataa aatattatac agggaaagaa tctttaaaag cagcaccttc tgatagacca
     1261 ataaaccaga aggaagcaga agaatttaga aaatatcttg agcatttgaa tgcccaggat
     1321 ttctggaaga aattaaaaga agaaacagga gaaccttgaa aggaggtttg tactatgtca
     1381 ggacataata aatgggcgag tatcaaacat aaaaaaatgg ctacggatgc aagaagggga
     1441 aagatgttta ccaaaattat ccgtgaaatc atgatggctg ctaaactggg tggaggaaat
     1501 cctgatagca atccacatct ccggatggcg attgaacggg ctaaaaatat caatatgccg
     1561 aatgataaca ttcagagggc aattaaaaag ggtaccggtg aagaaggtgg tgtgatgctg
     1621 gaatcagttg gttacgaagg atatggtcct ggtggggttg ctattcttgt ggaaacatta
     1681 actgataata aacgcaggac ggctgctgaa attcgttcaa tattctcccg tcacagtgga
     1741 aatcttggag aaagtggttg cgtatcgtgg atgtttgaga gaaaaggtgt tgtgacatta
     1801 aagaataaag aaatagatga agaaaaactc ctttctctcg ttcttgatgc aggcgcggaa
     1861 gacctgaaac ttgacaacga tgtatttgaa attgtgtgtg ctcctgataa acttgaatct
     1921 gtgaagaaaa tccttgaaga aaacaaaatt tctacagaaa gtgcttctgt caatctgatt
     1981 ccgaaaaata ccgtcagggt agaaggaaaa gaagcagagc aactgttgaa actgcttgat
     2041 gaacttgaag aacatgacga tgttcagaat gttcactcaa attttgatat tcctgacgca
     2101 ttgatcgagg cgatgtcccg ttgaaagtga acaatagtat cactgtgatg ggcgtggatc
     2161 ccggtctcaa cagttttgga tacgggataa tttctgaaga tacaagagca cttcatcctt
     2221 tgaaatatgg gcaacactct gttccaaagg gagtttcttt caatgataag cttgtttata
     2281 tcggagatac cttttcttca attattgatg aaattagacc tgattttata gcgatagaaa
     2341 agatttatgt ggctaaaaat actcaaattg ccctgtcaat aggaattgtt tcaggaatta
     2401 tcgcacacac tggtatttca aaaggggtac aggttgcatt tattacacca ggcgaaatta
     2461 aaaaaattat tacaggttct ggtcaggcaa tcaaacaaca ggtttgtttc atggttcaaa
     2521 aattacttgg tttgaaagaa gatccgggtg agcatgcaat tgatgccctt gcaacagcca
     2581 tctgttttat ttccataaac aggttcaata atctggtggg taaactatga tctgttcagt
     2641 ggaaggcaag cttgttaata aaacaccgat ggagattgtt gtagatgttt caggtattgg
     2701 ttatgttata aaagtaccac tatcaacttc cagcgttctt ccagaccagg gttctgtggt
     2761 caggcttttt accaccctca tgatcaggga agaaaatata gaactctatg gatttgcaac
     2821 agaaaaagaa tggaaaattt ttcagaaaat gattctgctc ccgggtgttg gtccaaagat
     2881 ggcattgagg atactttcaa gattgagtcc tgatgaaatt ctcaacgcag taatatctca
     2941 ggatattgat tttctcaaaa ggacaccagg tattggcaaa aaaacagcgg agcgacttgt
     3001 catggaattg aagcatcaca tagaaggtat tgaatttgtt tctgaagaac tttcaggaga
     3061 tttcattcct gaagcgattg aagcgttgac agttcttggc tacaagagaa acgatgcgca
     3121 acagattgtg agaaagaccg caaaagaaat tgagaagaaa cctgaaagcc t
//
LOCUS       DAORYU010000173         3162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_20409_length_3162_cov_3.979723, whole genome shotgun sequence.
ACCESSION   DAORYU010000173 DAORYU010000000
VERSION     DAORYU010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20409_length_3162_cov_3.979723"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..142)
                     /locus_tag="PLW07_05760"
     CDS             complement(<1..142)
                     /locus_tag="PLW07_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014097602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOL50067.1"
                     /translation="MNQMKKRVYQANILLPKYNLVVGTQGNVSGIDRKRNVVYIKPSG
                     VPY"
     gene            complement(139..1008)
                     /locus_tag="PLW07_05765"
     CDS             complement(139..1008)
                     /locus_tag="PLW07_05765"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOL50068.1"
                     /translation="MRQGKFFVNNWIFGDCEIEQVFERVSKIGYDGIELVGEPGIYHG
                     RSIVKLASVCGLKICSICGMFPGPEKDDLRALAHPEKKERKKAIEYIKRCIDLAKSVD
                     ARSVLVVPGLVGRPELFVSKDIDFKTTVDSIAEAAPYAESNKMYLTIEPINRYEVGLI
                     NSISEAIKMAKEINSPYVKIMGDTFHMQIEEPDGIPDAIRRAGKHWLYHMHAADNTRQ
                     APGRGTMDWRKILRSLYDIDYTGVISLEPLPKGASPYDARTGKIPAEILDRELSFALE
                     YLKLQESIVMEHL"
     gene            complement(1020..2729)
                     /locus_tag="PLW07_05770"
     CDS             complement(1020..2729)
                     /locus_tag="PLW07_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50069.1"
                     /translation="MNRYALFLILLSLFSVLNAQQFVEPLFDVKAILEQDLDARILNT
                     SVRDGIVLQEVEFTSEIYQGKPVRIYGIVGFPEGAKKLPAIFWSQAGMAPANKGMPEM
                     FAKRGYVCMCITLPHKIWNPWGAFNTKNPEDGNMTHFAIAQMRAITYLCSMPEVYPEK
                     IGIGGSSYGGFFSTFIAGADPRIKCGMSFFSGGNTSLGTHLPQFTNLETIEDIEVWNR
                     TIDPVLRLKFRKVPFLWAAASNDNWFYLPSVVKTYQDAIGEKRLAIVPLWEHGFPEEV
                     DEQLFSWFDIYLKQAKKPYNSVSHLTIKKSRDKLQASWSFSGHYEVKKAQLIVSYGKI
                     ELWKWWVHRNYVSFPAKIQDNVITVEIHVVEPDLEMLVFGNIIDENGAITSTETVQIK
                     AKDYGIKKANCTTTFNLFQWKVFDDETKKNFARMGLNRFIFDPEVKKGSPYSLRIEPY
                     GARKSTEISFKLHHVPLHSHRLKIWLKTEKPVEMVVTVKGIELVDSKSQIVKLLRKQE
                     YESKIPVFSISSDIDQNWKLVELDCPYQNEDIEGYNLHINAKGPVVYWIGSIVFEPVW
                     KKN"
     gene            complement(2739..>3162)
                     /locus_tag="PLW07_05775"
     CDS             complement(2739..>3162)
                     /locus_tag="PLW07_05775"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOL50070.1"
                     /translation="SPNAWRKYIKPSYKKFFSFCRNNGVHAGLHSDGWIVAIMEDLIE
                     CGLSLLNPQDLVNGIDNIERILKGRICIDLDIDRQNITYFGTPQDVEQHIFNCVKRLG
                     SKKGGLIFKFGAYPGTPIENVAAVIESFEKYHDWWIKQ"
BASE COUNT          902 a          722 c          488 g         1050 t
ORIGIN      
        1 catatggaac tccactgggt ttgatgtaaa caacatttct ttttctatca ataccactaa
       61 cattaccctg ggttcctacc acaagattat attttggcaa aagaatattt gcctggtaca
      121 ctcgtttttt catctgattc ataaatgctc catcacaatg ctctcctgaa gtttaagata
      181 ttcaagagca aaagatagtt ctctatccag aatttcagca gggatttttc ctgttcttgc
      241 atcataagga gaagcacctt ttggaagggg ttcaagggat atcacacccg tataatcaat
      301 atcatacaaa cttctcagta tttttctcca gtccattgta cccctgcctg gcgcctgcct
      361 tgtattgtct gctgcatgca tgtggtacag ccagtgtttt cctgcacgtc tgattgcatc
      421 aggaatacca tctggttctt caatctgcat atgaaatgta tcacccatga ttttgacata
      481 tggactgtta atttcttttg ccatctttat tgcttcactg atgctgttaa ttaatcccac
      541 ttcatatctg ttaattggtt caatggtaag atacatttta ttgctttctg cgtatggcgc
      601 tgcttctgct attgaatcca ctgttgtttt aaaatcaata tcttttgaaa caaataattc
      661 tggtcttcct accagcccgg gtacaaccag tacacttctt gcatccacac ttttagccag
      721 gtcaatacag cgtttaatat attcaattgc tttttttctc tccttttttt ctggatgcgc
      781 aagtgccctt aaatcatcct tttctggtcc tggaaacatt ccgcagattg aacatatttt
      841 taatccgcac acagaagcaa gtttcacaat acttctgccg tgataaatgc ctggctctcc
      901 aacaagttca ataccatcat aaccaatttt tgaaaccctt tcaaaaacct gctcaatttc
      961 acaatcaccg aaaatccagt tattgacaaa aaacttgccc tgtctcatat ttactccttc
     1021 taatttttct tccacaccgg ctcaaataca atgctaccaa tccagtaaac tacaggacct
     1081 tttgcattaa tatgaaggtt atacccttca atatcttcgt tctgatatgg acaatcaagt
     1141 tcaaccaatt tccagttctg gtctatgtct gacgatatcg aaaacactgg tatcttgctt
     1201 tcgtattcct gttttctcaa taatttcaca atctgcgatt ttgaatcaac cagttcaatt
     1261 cccttcaccg taacaaccat ttcaactggc ttttctgtct tcaaccatat tttcaatcta
     1321 tgactgtgta acgggacatg atgaagttta aaactaatct ctgtgctttt tctggcacca
     1381 tatggttcaa ttctgagtga ataaggactg ccctttttaa cttcagggtc aaaaatgaac
     1441 cgattaagtc ccattctggc aaaattcttt ttagtttcat catcaaaaac tttccactgg
     1501 aacagattaa atgttgtcgt acaatttgct ttctttattc cgtaatcttt tgcctttatc
     1561 tgtacagttt ctgttgaagt aattgctcca ttttcatcga taatgttacc aaatacgagc
     1621 atttcaaggt caggttcaac aacatgaatt tcgaccgtta taacattatc ctgtattttt
     1681 gcaggaaaac ttacataatt cctgtgtacc caccacttcc ataactctat ttttccataa
     1741 cttacaataa gttgtgcttt tttgacttca taatgccctg aaaatgacca gcttgcctgc
     1801 agtttatctc tacttttttt aatggtaaga tgggacacac tgttgtaagg tttctttgcc
     1861 tgtttcaggt aaatatcaaa ccaggaaaag agttgttcgt ccacttcttc tggaaaacca
     1921 tgttcccaca aaggaacaat cgcaagacgt ttttcaccaa tggcatcctg gtaggttttc
     1981 acaactgatg gaaggtaaaa ccaattgtca tttgaagcag ctgcccacag aaatggtact
     2041 tttctgaatt ttaatctcaa tacagggtca attgttctgt tccatacctc aatatcttca
     2101 atggtttcaa ggtttgtaaa ctgcggaaga tgtgtaccga gcgacgtatt cccacctgaa
     2161 aaaaaggaca taccgcattt aattctggga tctgctcctg caataaacgt ggaaaaaaat
     2221 ccaccatatg aagaaccacc aatacctatt ttttcaggat aaacctcagg cattgaacac
     2281 agatatgtaa ttgccctcat ctgtgcaatc gcaaaatgcg tcatgtttcc atcttcagga
     2341 ttttttgtgt taaaggcacc ccatgggttc cagattttat ggggaagagt aatacacata
     2401 caaacataac ctcttttcgc aaacatttca ggcataccct tgtttgctgg cgccatacct
     2461 gcctgactcc agaatattgc aggtaatttt tttgcgcctt caggaaaccc cacaatcccg
     2521 taaattctca caggttttcc ctgataaatt tctgatgtaa attccacttc ctgcaataca
     2581 ataccatccc tgacagatgt gtttaatatc cttgcgtcaa ggtcctgttc caggattgcc
     2641 ttcacatcaa ataatggttc gacaaattgc tgcgcattca acactgaaaa caaactcaac
     2701 agtattagaa aaagtgcata acgattcatt ctggctcttt attgctttat ccaccagtca
     2761 tgatattttt caaaactttc aatgacagca gcgacatttt caattggtgt accaggatac
     2821 gcaccaaact taaatataag ccctcctttt ttggaaccaa gtcgcttcac gcaattgaat
     2881 atatgctgct caacatcctg gggtgtaccg aaatatgtaa tgttctgtct atcgatgtca
     2941 aggtcaatac atattcttcc cttcaatatt cgttcaatat tgtcaatacc attaaccaga
     3001 tcctgaggat tcagcaaact gaggccacat tcaatcagat cttccataat tgcaacaatc
     3061 catccatcag aatgaagtcc tgcatgaaca ccattgttac ggcagaaact aaaaaacttt
     3121 ttatacgacg gtttgatata ttttctccag gcattcggac tg
//
LOCUS       DAORYU010000174         3157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_20452_length_3157_cov_4.328175, whole genome shotgun sequence.
ACCESSION   DAORYU010000174 DAORYU010000000
VERSION     DAORYU010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20452_length_3157_cov_4.328175"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="PLW07_05780"
     CDS             <1..329
                     /locus_tag="PLW07_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50071.1"
                     /translation="KDCPIPVVNPYQKKLDLAFDFTLASKRWAIVNDLAGVFAIDCAS
                     YLRKAYKKVIDGRADRKIFFEIPVKESQQNYLIENWKDPVFRNTYLNKYLKFSIEKYK
                     SCIKHK"
     gene            386..472
                     /locus_tag="PLW07_05785"
     tRNA            386..472
                     /locus_tag="PLW07_05785"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:420..422,aa:Ser,seq:cga)
     gene            complement(1171..1620)
                     /locus_tag="PLW07_05790"
     CDS             complement(1171..1620)
                     /locus_tag="PLW07_05790"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOL50072.1"
                     /translation="MKQKKTRVIFISIQNDARSQIAEGFLKHLAGDEFEVFSAGTVPA
                     TELNPLAVTVMTEKGIDISRNTPKSMLHFIPHTGIFDYIITVCDENEDVKCPAFTDKT
                     QKLHWNIKNPSSRFIGYDEQLDLMRKTRDQIYEKVKEFVKSVKYLKK"
     gene            1669..1968
                     /locus_tag="PLW07_05795"
     CDS             1669..1968
                     /locus_tag="PLW07_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50073.1"
                     /translation="MIFRKKALEKIKQIHRLSLLVNKQNHRKKLLHLANKHIIEIEQL
                     YSKKDPHADIETGDLAVLCFELILESNRNLDEVLEKCFSRYEKKLNMLAEQSKVQ"
     gene            complement(1955..>3157)
                     /locus_tag="PLW07_05800"
     CDS             complement(1955..>3157)
                     /locus_tag="PLW07_05800"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOL50074.1"
                     /translation="DWKKHLYIIWIAETISLIGFHFCLPFLPYYVQELGAKDLGEAAL
                     WTGLLQTVGPLMLVISSPFWGILSDRFGRKIMVERAMFSGFAILIAMSFVKNVQQLLV
                     LRILQGFFTGTFTAAIVLAASKLPKEKSGFGLGLMQTSVYVAATVGPFIGGVLSDIWG
                     YRNTFYFSGILVGIAGCMIMFGVQEPEKHQEAEELEQKNSNAKNNILFLLIVLFLFQC
                     AATFLSPVLAIFIQKISPASKKIATIAGSIIAASGIASVFAAIGAGHLAGKKDHRILL
                     AIMLGITGILTMLTSLCKTTGQLMALRVAAGLSAGGIITTINTHIHLGTRRASLGRNF
                     GVAGGISSLGAGIGPMIGGAIASMYSIESTFIFSGLMILLTGLICPVILEKTTIERKE
                     RLPEITEP"
BASE COUNT          934 a          663 c          641 g          919 t
ORIGIN      
        1 acaaagattg cccgattcct gttgtcaatc cttatcagaa aaaacttgac cttgcctttg
       61 atttcacgct tgcttcaaaa agatgggcta ttgtcaatga ccttgctgga gtgtttgcca
      121 ttgattgcgc ctcttatttg aggaaggcat acaaaaaagt gatagacggc agggcagaca
      181 gaaaaatttt ctttgaaatt cctgtgaagg aatcacaaca gaactattta atagaaaact
      241 ggaaagaccc tgttttcaga aacacatatt tgaacaagta cctgaaattt tctatagaga
      301 agtataaatc ttgcattaaa cataagtgag tccgtggacc tggtgcttgt ggaaaaaatg
      361 tgaaaaatcg ggtaaaaaat aatcaggaga ggtggctgag tggacgaaag cggccgactc
      421 gaaatcggct tcccgattta tcgggacgca ggttcgaatc ctgccctctc cgttctttct
      481 tcctcgaagg attcgaattg agcgaagtca ggcattgcga gcaagcgcag caatgccagc
      541 gaatggttgc cgatgcgatg aataaagagc attgtatggc aagcctgccg aggcggtgag
      601 gcagggtgga tcgacccgag acgtagtggg agacgagggc gccgagtcct gccctctccg
      661 ctttttttct tccttatcgt tttacccttt acaaaaccac ctttccaaat ttcccccttt
      721 gtaaaatctc tcctcttgtt ttcccccttt acgaaatatc tctttctgtt ctcccccttt
      781 acaaaagggg gactaagggg gatttcagga aatctccccc aacccctctt ttccctccgc
      841 cagttttccc ccttttcaaa agggggattg agggggattt caataaatct cccccaaccc
      901 ctcttttccc tccgccagtt ctcccccttt tcaaaagggg gattaagggg gatttcaata
      961 aatctccccc aacccctctt ttataaagag gggggtatta tgggatgctt ttacaaagag
     1021 gggggtagta tgaggtaatt ttacaaagag gggaggtagt atgaggtgat tttatataga
     1081 gggctggtgt gaagggtttt ataaagaggg cttaattaac gagaaatttt ggaaaggtag
     1141 taaacctgaa aagaagccct gaagcaaaaa ttattttttt aaatatttca cactttttac
     1201 aaattctttc actttctcgt aaatctgatc cctggttttt ctcattaggt caagttgttc
     1261 atcgtaacca ataaatcgcg aagaggggtt tttgatgttc cagtgcaact tctgtgtttt
     1321 atcagtaaaa gcagggcatt tcacgtcctc attttcatcg cacacagtga tgatgtaatc
     1381 aaatatccct gtatggggta tgaagtgaag catacttttt ggagtattgc gagaaatatc
     1441 tatacctttt tcagtcatga ctgtgactgc caaaggattg agttctgttg ctggtactgt
     1501 tcctgcactg aacacttcaa actcgtctcc tgccagatgt tttagaaaac cttcagcaat
     1561 ctggcttctt gcatcatttt gaatactgat gaatatgaca cgggtttttt tctgcttcac
     1621 tggattttcc ctctttttca atatagaata ccataaaaag ggagaaaaat gattttcaga
     1681 aaaaaagcac ttgaaaaaat aaaacaaatt catcgccttt cactattggt caacaaacag
     1741 aatcacagga aaaaacttct tcatcttgcg aacaaacata tcattgaaat tgaacaactg
     1801 tactcaaaaa aagacccgca tgctgacatt gaaacaggag atcttgctgt gttatgtttt
     1861 gaactcatcc ttgaaagcaa cagaaattta gatgaagtgc ttgagaagtg tttttcccgg
     1921 tacgaaaaaa aattgaatat gcttgccgaa caatctaagg ttcagtaatt tcaggcaatc
     1981 tttcctttct ttcaattgta gttttttcaa gaatcacagg acagatgagt cctgtcagga
     2041 gtatcatcaa tccactgaaa ataaaagtgg actctataga atacattgaa gcaatcgctc
     2101 caccaatcat tggtcctatt cctgctccga gtgaagagat gccgcctgca accccgaaat
     2161 ttcttccaag agatgcacgg cgggtaccaa ggtgaatatg ggtattaatg gtggtaatga
     2221 tgccgcctgc tgaaagcccg gcagcaactc ttaatgccat cagttgtcct gttgttttgc
     2281 aaagagacgt gagcattgtc aatattcctg taataccgag catgattgca agaaggattc
     2341 tgtggtcttt ttttcctgca agatgccctg caccaatagc cgcaaaaaca ctcgcaatac
     2401 ctgatgctgc tatgattgat ccagcgattg ttgcaatttt ttttgatgca ggcgaaattt
     2461 tctgaataaa aatcgcaagc actggcgaaa gaaacgtggc agcgcattga aaaagaaaaa
     2521 gtactatcaa aagaaacagg atattgtttt ttgcgtttga atttttttgt tcaagttctt
     2581 ctgcttcctg gtgtttttct ggttcctgta ctccaaacat aatcatacaa cctgcaattc
     2641 caacaagaat tcctgaaaaa taaaatgtgt ttctgtagcc ccagatgtct gataacaccc
     2701 cgccaataaa cggtccaaca gttgctgcaa catatacaga tgtttgcatc aatccaaggc
     2761 caaaaccact tttttctttt ggcagtttgc ttgctgcaag aacaatggca gcagtaaatg
     2821 tgccagtgaa aaaaccctgc aggattctta aaaccagaag ttgctgcaca tttttcacaa
     2881 aactcattgc tatcaggatt gcaaaaccag aaaacatcgc cctttcaacc atgatttttc
     2941 ttccaaacct atcagaaagt attccccaga aaggtgatga aatcaccagc atcagtggtc
     3001 ctactgtttg aagaaggcct gtccatagcg ctgcttctcc aaggtctttt gcgccaagtt
     3061 cctgcacata ataaggaaga aacggcaggc agaagtgaaa tcctatcaat gaaattgttt
     3121 cagcaatcca gatgatatac aggtgttttt tccagtc
//
LOCUS       DAORYU010000175         3148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_20538_length_3148_cov_3.210799, whole genome shotgun sequence.
ACCESSION   DAORYU010000175 DAORYU010000000
VERSION     DAORYU010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20538_length_3148_cov_3.210799"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="PLW07_05805"
     CDS             <1..467
                     /locus_tag="PLW07_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50075.1"
                     /translation="LGYPDAGAGSNDQGLLMYCEGNDKTGMIKHDDQYRGVAYCYLEN
                     IGPGGSAYNYVSPDFFRFVPWEGEGYKPVGYGPDSVSAIIGSIKHIEDNAGDNLQKRQ
                     GMIEEIDTKGIIATPANSFINELVVEAGRLSILNDGRPVKILYEPEPHVEFR"
     gene            complement(470..1660)
                     /locus_tag="PLW07_05810"
     CDS             complement(470..1660)
                     /locus_tag="PLW07_05810"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOL50076.1"
                     /translation="MIEKKLKWGIIGCGHRARGLVHIYHHLVQDSTVVAICDRIEALA
                     HKTKEILKDPDISIYTDHRKMLKESSVDAVSIFVAPEDNPDVVCECLEAGKHVLCEVP
                     LSLTLEGCWKIVVAVEKTGLKFQMAEQLRYAPFVKAWKKMIGEGLFGKILYCEGQYLH
                     GMGPDRYWMDVDTGERISIQQAEKYPGKLMKTRFWNLRHPIFYLPHELSPLLHILDDR
                     VVKVVGMATKSPGYRYDWFPQSDIEVALMHTEKDTVLRLMAGFVIETLKGSEHCNRLI
                     GTKGWVEQPRTKPEKGNMWFAEHLMSDAAQVEWNYANYWEYPSVAAGTGHGGLDWYPV
                     ANMVEAIIYDKQVEMDAYKAAETAAPAILAGISIEKGSIPVNVPDFRPCEKRKPGQFP
                     EKLY"
     gene            complement(1657..2682)
                     /locus_tag="PLW07_05815"
     CDS             complement(1657..2682)
                     /locus_tag="PLW07_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOL50077.1"
                     /translation="MKQGEFFEVKLGFINLVNKIGWKKALQKYKKDFKISENDAKKWW
                     KWYEKEICIPEVCYESDAGPQVVKGEKGTGEKLEKLPFIVTGPIKVEFMGNVYTLVNE
                     GLYRFSVITKSVRNLVMIKHKNSVIPILKAISLIQIHGGRDQSTTLEQRKKLLFSRYW
                     ISLTCGDISALTKQVLSDAGFTSRLVSALTLAQWNTYNNGHVLLEVFFPDISRWVLVD
                     VDMGYIFMHDGELLNAYSFWKCLKENNHILFVPLSQKEIDPLWLENEYYSYSHMWRRI
                     VKDVNTKLSWYKRIFQTFGFHTENKFIYIGDGKDARRIIEYRGHYSTTLPERAFVKKF
                     YCEKELK"
     gene            complement(2685..>3148)
                     /locus_tag="PLW07_05820"
     CDS             complement(2685..>3148)
                     /locus_tag="PLW07_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50078.1"
                     /translation="QIFVSDDVCLFNKSGLLPMTPDVYLKKIIAWLDINKDVYYQPFN
                     PIFHPYYLIKRPSTQEIFKQSVIHAKSSGIPVMNHRTFFSWWRKRSNIKLKYQLTDAC
                     IKFSGEKNFVDIGIVLPEKWNDKKVTGGIILKGTGEMLFSLNCRKIVYQKG"
BASE COUNT          898 a          668 c          544 g         1038 t
ORIGIN      
        1 gtcttggata ccctgatgct ggtgctggaa gtaatgacca gggacttttg atgtactgcg
       61 aaggtaatga taaaacaggc atgataaaac atgatgacca gtacaggggt gttgcgtatt
      121 gctatcttga aaatataggt cctggtggtt ctgcttacaa ttatgtgagt cctgactttt
      181 tcagatttgt accatgggaa ggcgaaggat ataaaccagt tggctatggt cctgactcgg
      241 tttcagccat tatcggttca atcaaacata ttgaagataa tgctggtgat aatctccaga
      301 agcgccaggg catgatagaa gaaattgata caaagggtat cattgcaaca cctgcaaaca
      361 gtttcatcaa tgaactggtc gttgaggcag gaagattgtc aatcttgaat gatggaagac
      421 cggtaaaaat cctgtatgaa ccagagccac acgttgagtt cagataacat caataaagtt
      481 tttcaggaaa ctgacctggt tttctctttt cacatggtct gaaatcagga acattaacag
      541 gaatactgcc tttttctatt gatattcctg caagaattgc tggtgctgca gtctcggctg
      601 ctttatacgc atccatttca acctgtttat cataaataat tgcctcaacc atatttgcaa
      661 caggatacca gtcgagaccg ccatgacctg ttcctgcagc cacagaagga tattcccagt
      721 aatttgcata gttccattct acttgtgctg catctgacat caaatgttct gcaaaccaca
      781 tatttccttt ttcaggtttt gtgcgcggtt gttccaccca gccctttgta ccaatcaaac
      841 gattgcagtg ttcactgcct ttaagggttt ctattacaaa acccgccatc agccgtaaaa
      901 ctgtgtcttt ttctgtgtgc attaaagcca cttctatgtc tgattgagga aaccagtcat
      961 acctgtatcc tggagatttt gtcgccatac ccacaacctt taccacccta tcatcaagaa
     1021 tatgcaagag tggacttaac tcatgtggaa gataaaatat cggatgtctc agattccaga
     1081 accttgtttt cataagtttg cccggatatt tttctgcttg ttgaatagaa atcctttctc
     1141 ctgtatcgac atccatccag tatctgtctg gtcccattcc atgaagatat tgaccttcgc
     1201 aatacagaat ctttccaaac aatccttctc caatcatctt tttccatgct ttcacaaaag
     1261 gtgcatacct taactgttct gccatctgaa atttcaaccc ggttttctcg actgctacta
     1321 caattttcca gcaaccttcc agtgtaagag acagaggaac ttcacataga acatgttttc
     1381 cagcttcaag gcattcacac acaacatccg gattgtcttc tggtgcgaca aaaatgctca
     1441 ctgcatcaac tgaagattct ttcaacattt tcctgtggtc tgtgtagatg gaaatgtcag
     1501 gatctttcaa tatttctttt gttttgtgtg caagcgcttc tatcctatcg caaattgcga
     1561 ccacggtact atcttgaaca agatgatgat aaatatggac aagccccctt gccctgtgtc
     1621 cacatcctat gattccccat tttagttttt tctcaatcat ttcaactcct tttcacagta
     1681 aaatttcttc acgaatgctc tttctggaag tgtcgttgaa taatgtcctc tgtattcaat
     1741 gattcttctt gcgtctttac catcgccaat atagataaat ttgttttctg tatggaaacc
     1801 gaatgtttga aaaatcctct tataccacga aagtttggta ttgacatctt ttactattcg
     1861 ccgccacata tgggagtagg agtaatactc gttttcaagc cataatgggt ctatttcctt
     1921 ctgggaaaga ggaacaaaaa gaatatggtt gttttctttt aaacatttcc agaatgagta
     1981 tgcgttaaga agttccccgt catgcatgaa gatatatccc atatcaacat caacaagaac
     2041 ccatcttgat atatcaggga agaaaacttc taaaagaaca tgtccattgt tataggtatt
     2101 ccattgagca agagtcaaag cactgacaag ccgtgaagta aaccctgcat ctgaaagaac
     2161 ctgttttgta agggcagaaa tatctccaca ggtaagtgaa atccagtacc ttgaaaaaag
     2221 caattttttt cgctgctcca gtgtggttga ctggtccctt cctccgtgaa tctgtatcag
     2281 ggaaattgct tttaatatcg gtatcactga atttttatgt tttatcataa caagatttct
     2341 cacgcttttt gtaatcactg aaaatctgta aagcccttcg tttactaagg tgtaaacatt
     2401 tcccataaac tcaactttaa tcggccctgt tacaataaaa ggcagttttt caagtttttc
     2461 gcctgtaccc ttttctcctt tgacgacctg tggtccagca tctgactcat aacaaacttc
     2521 aggaatgcat atttctttct cataccattt ccaccatttt tttgcgtcat tttctgaaat
     2581 tttgaaatcc tttttatact tttgtaatgc tttcttccag ccaatcttat tgacaagatt
     2641 aataaatcca agtttgactt caaagaattc tccttgtttc atttttaccc cttttgataa
     2701 acaattttcc tgcaatttaa cgaaaaaagc atctctcctg ttccttttaa tatgatacca
     2761 cctgtcactt tcttatcatt ccatttttcg ggtaagacaa tcccaatatc tacaaaattt
     2821 ttttcacctg aaaattttat gcaagcatca gttaattgat atttcagttt gatgttgctt
     2881 ctttttctcc accacgagaa aaatgtcctg tgattcatta ctggaatgcc gcttgatttg
     2941 gcatgaataa ccgattgttt gaaaatttcc tgcgttgaag gtcgcttgat gagataataa
     3001 ggatgaaaaa tcggattgaa tggctgataa taaacatctt tgttgatatc aagccaggca
     3061 atgatttttt ttaaatatac atcgggtgtc atcggcaata gcccactttt attaaataga
     3121 cacacatcat ctgaaacaaa aatttgaa
//
LOCUS       DAORYU010000176         3120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_20775_length_3120_cov_9.209135, whole genome shotgun sequence.
ACCESSION   DAORYU010000176 DAORYU010000000
VERSION     DAORYU010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20775_length_3120_cov_9.209135"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2332)
                     /locus_tag="PLW07_05825"
     CDS             complement(<1..2332)
                     /locus_tag="PLW07_05825"
                     /inference="COORDINATES: protein motif:HMM:NF020121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HOL50079.1"
                     /translation="MKTFLSFAIFFLLANICFSFTIEIKVNNTSDIPVSCSISLVRSY
                     TDEYGNWWPHFGYVTKDGLVKQKQDIKNFIQPGTQSDWIKLPADGRVLVCTKISEKPV
                     PVGIDVKLIDNENTISKSFVIPETQETYYIYLYTVPSSPGPIKIEPLSAQQIVEKNLE
                     IVKQATENKKSKLPSKIAIYTDCHLLPTDPSHYRKSQYKLLRELGINGIMCAASDLLP
                     EIIDNGFLYLRSGGASISYNDYYKTAEEIKPFLKNGALNQSGIFERYNLIEKVRNLKL
                     GDEILSGLLQEYLACGEKTRVEIIDYLKEKHIPLSDLNVSSYQEISIKPAAIMKMENP
                     GLYYWTNRIRMERINSLWKCAMEVNKKYFPSAWGSPNWPVGGYLDGTYEGQGWDLWHL
                     YKNKFLDGIWGEDWPGYEVWLRGGNAFLVDMMRCQAKGLPMGIYNVVETNYSPVYARY
                     KFYEQLINGITEIFWYSYGCLRGNESNPWEIKTDMVGEIALLNRDAGEAEEYILNTGL
                     EPASVAILWTPAQEIWDPQYHVEIMALYYVLLHSNYSVDFISSYDIESGDLEKYKVLY
                     MPFHYVENNVWEKIKKWVANGGFVVIEGGYLKDECNRNIDLGLWIKGFSSQIETKTQS
                     IGRLPLELPQQKLLDTTQSVSFPVVCLKSTLEIPDNGTCLLTYKDGSPAAIQVKKGKG
                     NVRITGFLQGIAYVYDQGKRDMAKWGDVLLYHSFSPEIRKFVLDPVYQSNIQKVCNID
                     KDLIIARKRTGKNHQCIAIFDYGFGSKKPVMPVWSEI"
     gene            complement(2386..>3120)
                     /locus_tag="PLW07_05830"
     CDS             complement(2386..>3120)
                     /locus_tag="PLW07_05830"
                     /inference="COORDINATES: protein motif:HMM:NF014571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046353 - aminoglycoside
                     3-N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HOL50080.1"
                     /translation="LMELIGNQGILMMPSFNQGVIFEKNPKAYFSPPETPTIDGAIPD
                     TFWRMKGVYRSLNPTHSFAVWGKDAIEFVKDHHKVLTMGTNSPLHLLEKQGGKIVLID
                     TPSANTFHHVVEMTNNVPCLGKRTEEYPVKLPSGKFVKVRTWGWRNGTCEITDKAVYF
                     DYMLKHNLLKKGKLGNASVYVLDMKVCRKVIENFLKGKIKRYPGCKKCKIRPRKTPHT
                     VESDWDQIHQKVKPDTPAFTGDYEIA"
BASE COUNT          881 a          642 c          527 g         1070 t
ORIGIN      
        1 caatttctga ccagacaggc attactggtt ttttactacc aaacccatag tcaaaaattg
       61 caatacactg gtgattttta ccagttcttt ttcttgcaat gattaaatcc ttatcaatat
      121 tgcatacctt ttgaatgttt gattgatata ctgggtcaag tacaaatttc ctgatttcag
      181 gtgaaaaact gtgatataaa agaacatcac cccattttgc catgtccctt tttccctggt
      241 catatacata ggcgattccc tgcaaaaatc cagtaattct tacattgcct tttccttttt
      301 taacctgtat ggctgcagga cttccatctt tgtatgtaag aaggcaggtg ccattatcag
      361 gtatttcaag agtacttttc agacatacca cagggaaact tactgattgg gttgtatcaa
      421 gtaatttctg ctggggaagt tccaatggaa gccgacctat tgactgtgtc tttgtttcta
      481 tttgagacga aaatcctttg atccataaac ctaaatcaat gtttctattg cattcatcct
      541 taaggtaacc gccttcaatg acaacaaatc caccatttgc cacccatttt ttaatttttt
      601 cccatacatt gttttcaaca taatgaaatg gcatatacag taccttatat ttctcaaggt
      661 cccctgattc aatatcataa cttgatatga aatcaacact gtaatttgag tgtaagagca
      721 cataataaag agccataatc tcaacatggt actgtgggtc ccatatttcc tgcgctggtg
      781 tccagagtat tgcaacagat gctggttcaa gtcctgtgtt taatatgtat tcttctgctt
      841 ctcctgcatc tctatttaac aaagcaattt caccaaccat gtctgttttt atttcccagg
      901 ggtttgactc atttccacgc aggcatccat aactgtacca gaaaatttcg gtaatgccat
      961 tgattaactg ttcgtaaaat ttatatcttg cgtacacagg agaatagttt gtctcaacga
     1021 cattataaat tcccattggc agtccttttg cctgacatct catcatgtca acaagaaatg
     1081 catttccacc tcttagccac acttcataac caggccagtc ctcaccccag ataccatcaa
     1141 gaaatttgtt tttatacaga tgccacaggt cccatccctg accttcataa gtaccatcaa
     1201 gatacccccc aacaggccag tttggacttc cccaggcaga tgggaagtat ttcttgttca
     1261 cttccatggc acacttccat aagctgttta ttctttccat tctgatacgg tttgtccaat
     1321 agtaaaggcc agggttttcc attttcataa ttgctgctgg ttttatagaa atctcttggt
     1381 acgacgaaac attcaaatcc gaaagaggta tatgtttttc tttcaaataa tcaatgattt
     1441 cgacccgtgt tttttcacca cacgcaagat attcctgtaa taatccagat aaaatctcat
     1501 ctccaagttt taagtttctg actttttcaa taagattata cctttcaaat attcctgatt
     1561 gattaagcgc accatttttt agaaacggtt ttatttcttc agctgtttta tagtaatcat
     1621 tataactaat ggatgcgcca cctgacctta aatacagaaa tccattgtca ataatttcag
     1681 gcaacaaatc gctggcagca cacataattc cattgatacc aagttccctt aaaagtttgt
     1741 actggctttt tctgtagtga gaggggtctg tcggcagaag atgacaatct gtatagatag
     1801 cgatttttga tggaagtttg ctctttttgt tttctgttgc ctgtttcaca atttcaagat
     1861 ttttttcaac gatttgttgg gctgaaagtg gttcaatctt tataggaccc ggagatgatg
     1921 gaactgtgta aagatagata taatatgttt cctgggtttc aggaataaca aaggattttg
     1981 agatcgtgtt ttcgttatct ataagtttta cgtcaatacc aactggtaca ggtttttcgc
     2041 taatttttgt gcatacaaga actcttccat ctgcggggag ttttatccag tcagattgag
     2101 tacccggctg gatgaagttt ttaatgtcct gtttctgttt taccaaacca tcttttgtca
     2161 catatccaaa atgaggccac caattgccgt attcatcagt ataggacctc acaagagaaa
     2221 tagaacaact tacagggata tcagaggtat tgttaacttt gatttctatt gtgaatgaaa
     2281 aacaaatgtt agccagtaaa aagaaaatcg caaaactcag aaatgttttc atagaaatta
     2341 ttctatacca ggtgcctgat attgacaaac caaaagaaaa aaaacttatg ctatttcata
     2401 atcaccagta aaagcaggtg tatctggttt cactttctga tgaatctggt cccagtcgct
     2461 ttcaacagtg tgcggtgttt ttctgggtct gattttgcat tttttgcatc caggatatct
     2521 ctttattttt ccttttaaaa aattttcaat gacttttctg catactttca tgtctaacac
     2581 ataaacactg gcatttccaa gtttcccctt tttcaacagg ttatgtttca acatgtaatc
     2641 aaaatatacg gccttgtctg taatttcaca tgttccattt ctccatcccc atgttctgac
     2701 ttttacaaat tttcctgatg gaagtttcac tggatattct tctgttcttt ttccaaggca
     2761 cggcacattg tttgtcattt caaccacatg gtgaaaggta tttgcactcg gcgtatcaat
     2821 gagaacgatt tttccgccct gtttttcaag gaggtgaagg ggactattgg ttcccattgt
     2881 aagaactttg tggtggtctt tcacgaattc tatggcatct tttccccaga cagcaaagga
     2941 atgagtggga tttaaacttc ggtaaacacc cttcattctc cagaatgtat cagggattgc
     3001 gccatcaata gttggtgttt cagggggcga aaaatatgcc ttgggatttt tttcaaaaat
     3061 cactccttga ttaaatgagg gcatcatcaa aatcccctga ttaccaatca attccatgag
//
LOCUS       DAORYU010000177         3116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_20804_length_3116_cov_5.159425, whole genome shotgun sequence.
ACCESSION   DAORYU010000177 DAORYU010000000
VERSION     DAORYU010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20804_length_3116_cov_5.159425"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..957
                     /locus_tag="PLW07_05835"
     CDS             154..957
                     /locus_tag="PLW07_05835"
                     /inference="COORDINATES: protein motif:HMM:NF039204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5715 family protein"
                     /protein_id="HOL50081.1"
                     /translation="MTKQRIIVLGVVVILVAMVVFLCIFTYYRISLPVAKLKKEAAKY
                     QEYRKNFAENRFFLNASEVKDLRIMVNDYHVEVAQKLGVPGLIDDEAVSQAAKEGKLV
                     SLKENRYWRIKELKDSLAYVTPDTIKLLNLIGERFQKNLKKQNLPLYRFTISSVLRTQ
                     QAQEKLVRKNRNATRNISSHQFGTTVDILFTEFEYTADHQFTFACLSKYKERPEFRKK
                     EFDELASIYGQCLKTILAKTLLDLQKEGICYVIYERRQPVFHVTIATKF"
     gene            complement(1015..>3116)
                     /locus_tag="PLW07_05840"
     CDS             complement(1015..>3116)
                     /locus_tag="PLW07_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50082.1"
                     /translation="YYDPKNPEATQKWLIEELKKYMVEKSPINKPEYILFFPEPGIGP
                     ITYGIWPTHYNEPDYQLSESEQKSFQAQLEKFLLGAKAVRKEWPDVKILIPHGDPHYT
                     ALFLRFSPEARELIDGVGLDLPGFERTPEQPVHQVVLNRLYPILQDIKRYKPNPYLVV
                     VEGTCISSADYDTSFEDQANIATRNFLILIGYGINNHASSNAPFDCANYWGENHYGGG
                     YCSRLPLAMPKLAYVSYATLTRHINRCNFIRYVPTGSTSTYCQEYRHYKTGKLVYVLW
                     TIRGKRPVTIKLDPGQSVEVYDINDNPVILKENNGIATFTIGQSPVYLEGLTKPIELV
                     LGEPDHSDSLPSRISVKLGNLGDGSWKISKVRDTEYENTNKLQIERFSGEMSIAQVKT
                     DKKFGAKALSVHLDKQPIDRKVMPYYTTFVPAKPIVIPGKASYIGMWVNAASDWGRVV
                     YVLRDAKGEKWISVGTKDDWNSDDIRTASFFCFDGWRYMKFQLPSNAPYDCYRELGTS
                     WWGSYGGDGIVDLPLKLEKIIVERRSSVIYGNQLIPANNSDVLLADLFAEYENKSDAT
                     AEAIKLSKLRMPVPQGIPGLENPISKMEKEGVLPSAKILKVEDPEQMPDGTRCHIYFA
                     EIENAKSYNVWASVYPDGTGAVKIASNIVKSGALIQGLKPDTDFYLFITYSDSEGKLS
                     KPSQPFKVNLKNKFLYQ"
BASE COUNT          957 a          610 c          553 g          996 t
ORIGIN      
        1 tttgaatttt tttcattatg ctttttcatt gttcccccat ttagaaaaat aatacccttt
       61 taatggaaaa aaatcaatag aagttttgcg aattttttgt tttttgtgta gaattcatac
      121 ggacttaatg aaatttttat ataaaggtga aatatgacga aacaaaggat tattgttctt
      181 ggcgtagtag tcatacttgt cgcaatggta gtttttcttt gtatattcac atattacagg
      241 atttcattac ctgtcgctaa gttaaaaaaa gaggcagcaa aataccagga atatagaaag
      301 aattttgctg aaaatcgttt cttccttaat gccagtgaag taaaagattt gaggataatg
      361 gtcaatgact atcatgttga agttgctcaa aaacttggtg taccaggact gattgacgat
      421 gaagcagttt ctcaggcagc aaaggaagga aaacttgtat cattaaaaga aaacaggtac
      481 tggaggatca aagagttaaa agattctctt gcctatgtta cgcctgatac cataaaattg
      541 ctcaatctca tcggagaaag attccagaag aacctgaaaa aacaaaatct tcctttatat
      601 cgttttacaa tcagttcagt gttaagaacc caacaggcac aggaaaaact tgtaaggaaa
      661 aacagaaatg cgacaagaaa catatcaagt catcaattcg ggaccactgt tgatattctt
      721 tttacagaat ttgaatatac agcagaccat caatttacat ttgcctgcct tagtaaatat
      781 aaagagagac cagaattcag aaagaaggag tttgacgaac ttgcgtcgat ttatggacaa
      841 tgtttgaaaa caatactggc aaaaacactg ctggatttac aaaaggaagg catttgctat
      901 gtgatttatg aaagacgcca gccagttttt catgtcacaa ttgccacaaa attttgacta
      961 tcataaacta tttttctaca tgatataaag acaaccgaaa aaacccattg ttgctcactg
     1021 atataaaaat ttattcttca gatttacctt gaatggctga gatggtttcg ataatttacc
     1081 ttcgctatca ctgtaggtta taaacagata aaaatcagtg tcaggtttca aaccctgaat
     1141 aagagcacct gactttacaa tatttgatgc aattttcact gctcctgtgc catcaggata
     1201 cacagatgcc cacacattat aagattttgc attttctatc tctgcaaaat agatgtggca
     1261 ccttgttcca tcaggcattt gctctggatc ttctaccttc aaaattttcg ctgatggaag
     1321 tacgccttct ttttccattt tactgatagg attttcaaga cctggaattc cctggggcac
     1381 tggcattctc agtttggaca gtttgattgc ttctgctgtt gcgtcagact tattttcata
     1441 ttcagcaaaa aggtctgcca gaaggacatc tgaattgtta gcaggaatca gctggtttcc
     1501 ataaatcact gatgagcgcc tttcaacaat aatcttttca agtttcaaag gaaggtccac
     1561 aatcccatca ccaccgtaag acccccacca acttgttccc agttctctgt aacaatcgta
     1621 tggtgcattt gatggtaact ggaatttcat atatctccat ccatcaaagc agaagaaact
     1681 tgcagtcctt atatcatcac tgttccaatc gtcttttgtt cctacactaa tccacttttc
     1741 accttttgca tctcttaaaa cataaaccac ccttccccag tcgcttgctg cattaaccca
     1801 cattccaata taacttgctt ttcctggaat tacaatgggt tttgcgggca caaaagttgt
     1861 gtaatagggc atcacctttc tgtcaatcgg ttgtttatca agatgaaccg acagggcttt
     1921 tgcaccaaat ttcttatcag ttttcacctg cgcaatagac atctcgccag aaaatctttc
     1981 tatttgaagt ttatttgtat tttcatattc agtatcccga acttttgaaa tcttccatga
     2041 accatctcct aaattaccaa gtttcactga aattcttgac ggtaaactgt cgctgtgatc
     2101 tggttctcca agaacaagtt caatgggttt tgtcaatcct tcaagataaa caggagattg
     2161 tccaattgta aaggttgcga ttccattgtt ttccttcaaa atcacagggt tatcgttaat
     2221 atcatacact tcaacagatt gtccagggtc aagttttatt gtcacaggcc ttttacccct
     2281 tattgtccac aacacataaa caagtttccc tgttttataa tgacggtatt cctgacagta
     2341 tgttgaagta ctgcctgtgg gtacatatct gataaagtta catctgttaa tatgccgcgt
     2401 aagtgttgcg taagaaacat aggcaagttt cggcattgca agtgggagac ggctgcaata
     2461 tccaccacca taatgatttt caccccagta attggcacaa tcaaaaggag catttgaact
     2521 tgcatgattg tttatgccat aaccgataag aataagaaag tttctggttg cgatattcgc
     2581 ctgatcttca aaacttgtat cataatcagc agaagaaata caggttcctt ccaccaccac
     2641 aagatatgga tttggtttat atctcttgat gtcctgaaga ataggataga gacggttcaa
     2701 aacaacctga tgaactggtt gctcaggtgt tctttcaaaa ccaggtaaat caagtccaac
     2761 tccatctatc aattctcttg cttcagggct gaatcttaaa aacaatgcgg tataatgcgg
     2821 gtcaccgtga ggtatcagaa tttttacatc aggccactct tttctaacgg cttttgcgcc
     2881 aagcaagaat ttttcaagtt gtgcctgaaa agatttttgt tcggattcag aaagttgata
     2941 atctggctca ttgtaatggg tgggccagat accataagta ataggaccga ttcctggttc
     3001 agggaaaaac agaatgtatt ctggcttatt aatggggctt ttttccacca tatacttttt
     3061 caattcttca atcaaccatt tctgcgttgc ttctggattt ttggggtcat aatatt
//
LOCUS       DAORYU010000178         3106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_20888_length_3106_cov_6.657817, whole genome shotgun sequence.
ACCESSION   DAORYU010000178 DAORYU010000000
VERSION     DAORYU010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20888_length_3106_cov_6.657817"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="PLW07_05845"
     CDS             <1..311
                     /locus_tag="PLW07_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50083.1"
                     /translation="ITAINKPKWKIFGTKGAIEVNWDTIDRINLVSFVSGVKQEGIVK
                     ITPSYGHTEYYRNIADHLLMEEELIVKPEQSRRVIGVVEAAEKASKLNKILPPFENCE
                     "
     gene            308..1681
                     /locus_tag="PLW07_05850"
     CDS             308..1681
                     /locus_tag="PLW07_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50084.1"
                     /translation="MKGERIININGVFPHLAVKAEHLPARTETGIGALIPWTNKLWFI
                     TYVAHKKGTGAGTGLFTIDDDFKIEKHPASRVGTYANRLIHAETNQLIIGPHIIDTEG
                     HVRTFEELVDVRIAATARHLKNPAEKVYFLGMEGEFIEADVKTLKVKFLYDLNRELGI
                     KRPHFKDAFTGHNRVVVANNSYYPEDFEKAESDGRLAEWDGREWKIIDRTQFNAVTGR
                     YTKLGDAIYATGQDRCSALFYVFLPETGWKCYRLPKGTHTQDHAFTTEWPRIREIESE
                     RWLMDASGIFYELPAMSYANCVWGIVPVCRHLRIIPDFCCWNGLLVLAGDQTTPIGDT
                     NPFVGQPQANLWFGKTDDLWKFGKPSGWGGPWWETPVKPKIPSDPFLMTGFSISCLHI
                     SHNGNASNRFTVETDFQGTGIWHPFTEIKTKKGYGFLNFPAGFSAHWVRIVPHARCVA
                     TAQFFYT"
     gene            1693..2994
                     /locus_tag="PLW07_05855"
     CDS             1693..2994
                     /locus_tag="PLW07_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50085.1"
                     /translation="MPDTDTLLAGVAEIDITPPVGTFLAGSLKPRHSTGIEDPLYVKA
                     ISIQSGKNKLVYVIFDLIALDRNTAEQGMKLTYEKTGILPETIVWAASHTHTGPYTGG
                     LFFDESLLDRKWLNTIPEKMAQCIAMADKNKKVVRFSKLRSFHYGLSHNRRILFKNGR
                     VINTWLLHNAPEDVQSIGSAGPIDPEIGIFCFEDMEGMPVAIIFHFTLHTNTNFGSKF
                     SADYPGVVAARIRERFGPDVITLSMPGACGDINSTGLRYRQVGDALAEKIISALETRK
                     PETRPVFLGVKKCEVTVPIRDFSVDQTDRIASSGWDEESQKVFFNELEIMRKTGRKED
                     TTVLQAWCIDDVGFASLPGELFVEWGLKIKKESPFPFTYPVELGGDYLGYLITHKAWQ
                     EIGYEALIARSARPAPEGVEKMVQRVIEMLFELYREKGERK"
BASE COUNT          986 a          570 c          721 g          829 t
ORIGIN      
        1 ctataacagc cattaacaaa ccaaagtgga aaatttttgg aacaaaagga gccatagaag
       61 tcaactggga taccattgac agaatcaacc tggtaagctt tgtttcagga gtaaaacagg
      121 aaggaattgt aaaaatcaca ccatcctacg gacatactga atattacaga aatatcgctg
      181 accatctatt gatggaagaa gaattgattg taaaaccaga acagtccaga agggtaattg
      241 gggtagttga ggcggcagaa aaggcatcga aacttaacaa aattctgcca ccatttgaaa
      301 actgcgaatg aaaggtgaaa gaataatcaa tatcaatggt gtttttccac atctggccgt
      361 gaaggcagag catcttcctg caagaactga aacaggcatt ggtgctttaa taccctggac
      421 aaacaaactg tggtttatta cctatgttgc gcataagaaa ggcacagggg caggaacagg
      481 actttttaca atagatgatg atttcaaaat tgaaaaacat ccagccagca gggttggcac
      541 atacgccaat cgtttgatac atgctgaaac caaccaactc attattggtc cccacattat
      601 tgatacagaa ggtcatgtcc gtacttttga agaattggtt gatgtccgta ttgccgccac
      661 tgcgagacac ttgaaaaatc cggctgaaaa agtatatttc ctcgggatgg aaggcgaatt
      721 tattgaggca gatgtaaaaa ccttaaaggt gaaatttctc tatgatttga acagagaact
      781 tggcattaaa agaccgcatt tcaaggatgc ttttacaggg cacaacaggg tagttgtggc
      841 caacaactct tattatccag aagattttga aaaggcagaa agcgatggaa gattggcaga
      901 atgggacggc agggaatgga aaataattga cagaactcaa tttaacgctg ttactggaag
      961 gtatactaaa cttggcgatg caatttacgc aaccggtcag gacagatgct ctgctttgtt
     1021 ctatgtgttt ctgcctgaaa ctggatggaa atgttacaga ttaccaaaag gcactcatac
     1081 ccaggaccat gcctttacaa cagaatggcc aagaatccgg gaaattgaga gtgaacgctg
     1141 gttaatggat gccagtggta ttttctacga gctgccagca atgagttatg caaactgtgt
     1201 gtggggtatt gtcccggttt gcagacacct gaggattatt cctgatttct gctgctggaa
     1261 tgggctgctt gttctggcag gagatcagac cacgccaatt ggtgacacaa atccattcgt
     1321 cgggcaaccc caggcgaatc tatggtttgg aaaaacagat gacctgtgga aattcggcaa
     1381 accatctggt tggggtggtc catggtggga aacacctgta aaaccaaaaa ttccatcaga
     1441 cccatttttg atgacaggtt tttcaatctc ctgcctgcat atctctcaca atggcaatgc
     1501 ttctaatcgt tttacagtag aaactgattt ccagggtaca ggaatctggc atccttttac
     1561 agaaataaaa acaaaaaagg gctacggatt tttgaatttt ccggcaggct tttccgcaca
     1621 ctgggtccgg attgtacctc atgccaggtg tgtggcaact gcccagtttt tctatactta
     1681 aaaggatatt gaatgcctga tactgataca ttgctggcag gcgtggctga aatagatatc
     1741 actccccctg tcggaacgtt tcttgctggt tcattgaaac caaggcattc cacaggtatt
     1801 gaagaccctc tttatgtgaa ggcaatttcc atccagtcag gaaaaaacaa acttgtatat
     1861 gtaatatttg accttattgc cctggataga aacaccgctg aacagggcat gaaactaacc
     1921 tatgaaaaaa caggtattct gccagaaacc attgtatggg cagcaagcca cacccacaca
     1981 ggtccatata caggtggatt attttttgat gaaagtttac ttgacaggaa atggttgaat
     2041 actattccgg aaaaaatggc gcaatgcata gcaatggctg ataaaaataa aaaggttgtt
     2101 aggttttcaa agttgagaag ttttcactac gggctatctc ataatagaag gattttgttc
     2161 aaaaatggac gtgtgatcaa tacatggctt ttacacaatg ctccagaaga tgttcaatcc
     2221 ataggaagcg ctggtcccat tgaccctgag attggaattt tttgttttga ggatatggaa
     2281 ggaatgccag ttgccattat ttttcatttt acccttcaca caaatacaaa tttcggttcc
     2341 aaattcagtg ctgattatcc tggtgttgtg gcagcaagaa taagagaaag gtttggtcca
     2401 gatgtgatta cactttctat gcctggagca tgcggagaca ttaactcaac aggtctacga
     2461 tacaggcagg tgggagacgc gcttgctgag aaaattattt ctgctcttga aacaagaaaa
     2521 ccagaaacac gtcctgtttt tctcggcgta aagaaatgtg aagtgactgt gccgatacgg
     2581 gatttttcgg ttgaccagac agatagaatt gccagttctg gctgggatga agaatctcag
     2641 aaggtctttt tcaatgaact tgaaatcatg cgaaagacag gaaggaaaga agatacaaca
     2701 gtacttcaag catggtgcat agatgatgtg ggttttgctt cattgccagg agaacttttt
     2761 gttgaatggg gattgaaaat aaaaaaagaa agtccttttc catttacata tccagttgaa
     2821 cttggtggag attatctggg gtatcttatt acacacaagg cctggcagga aatcgggtat
     2881 gaagcgttaa ttgcaagaag cgcaagacct gctccagaag gagtggaaaa aatggttcaa
     2941 agagtgattg aaatgctatt tgaactttat agggaaaagg gggaaaggaa atgaaaaaat
     3001 tgaaaattgg aattgctgga ctatcacggg gtagaggttt tgtcagtgtt ttttcgtctc
     3061 atcctgatgt cactatttca gcaatatgcg acatagacca gaaaaa
//
LOCUS       DAORYU010000179         3052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_21394_length_3052_cov_3.583250, whole genome shotgun sequence.
ACCESSION   DAORYU010000179 DAORYU010000000
VERSION     DAORYU010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21394_length_3052_cov_3.583250"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="PLW07_05860"
     CDS             complement(<1..682)
                     /locus_tag="PLW07_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOL50086.1"
                     /translation="MIEKKISQKAKNIKPSSTLAVNKKAMELVATGEKIINLSVGEPD
                     FDTPDFIKKSAINALNEGFTKYTDASGVKPLRQAICEKFKRDNNLAYEPDQIVVSCGA
                     KHSLFNVLFALCDEGDQVIIPAPYWVSYPEMVKLAGGTPVICRYKDNFKMDIAHLQEI
                     ISPKIKALILNSPSNPTGIVYTKDELERIAEIALKHQIVVISDEVYEHLVYGGLKHTS
                     IAFLNDEMK"
     gene            814..1617
                     /locus_tag="PLW07_05865"
     CDS             814..1617
                     /locus_tag="PLW07_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOL50087.1"
                     /translation="MTCIAVINQKGGVGKTTSVVNLGAGLSLLGKKVLMIDLDPQSHL
                     TYSVGIKAHELSKTVYHVFRSDTSVSDAIVERQISVHLAFPEIKETTGVFFILPSSLD
                     LSGAEFEFGGIAGREFLLKECLSEAGDFDYVFIDCPPSLGLLTLNALTTADEIYIPLQ
                     TEFLALQGISKLLETVDVVQKRLNQRIKLTGIIGTRFDGRKRLNREVVEKIKDYFGGR
                     VFRTLIRENISLAEAPGFGKTIYEYKPDSYGSEDYFHLCLEIIERGKKH"
     gene            1633..1929
                     /locus_tag="PLW07_05870"
     CDS             1633..1929
                     /locus_tag="PLW07_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50088.1"
                     /translation="MAEKKLTRLGSDPLRWIQDSRKQETADSSGKKKIQQKQGVPKTK
                     EGLKEGWIRATFIVREEHLEKIKAVSYWDRKDIKQVVDEALGEYLKNKRVKPRP"
     gene            1946..>3052
                     /locus_tag="PLW07_05875"
     CDS             1946..>3052
                     /locus_tag="PLW07_05875"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HOL50089.1"
                     /translation="MSDTRKLPVTIKNFFSKINESVTKARNSEHLLTLISKIAVDEFD
                     ADRASIFLRDEKTDELRLIAGTGISEEIVRSHPVAQQQNISYWVAENKKPIILKGSVK
                     KDKRFQSQSTTVIASSIIVPLIFENSVVGVFNLSRLAEGRPDFTEEDMTLFRFLGDLV
                     VISLQLLITQEKRIHSEQLAAIGLATAELVHSLKNLLVGIFGAAELVDVLMQQNDWDS
                     VKENWSLLADNIKNISTVVNEVLSYSKSSSITKQELDIREIVESLCNFIKPRCNISNI
                     QLFFQCGDEKILVWGNRESLYNALMNILDNAIRAMPDGGKLNISCMIEDDMVRIEISD
                     TGVGIPEENLEKIFEPFFTTDKKKGTGIGLALTKK"
BASE COUNT          956 a          533 c          645 g          918 t
ORIGIN      
        1 ttttcatttc gtcgttcaga aaagcaatgc ttgtgtgctt taacccgcca taaacgagat
       61 gttcatacac ttcatcagaa attacaacga tttgatgttt gagtgcaatt tctgcgatgc
      121 gttcaagttc gtctttcgta taaacaattc ctgtaggatt tgatggactg ttaagaatga
      181 gtgcctttat ttttggggaa ataatttcct gtaagtgggc gatgtccatt ttgaaattat
      241 ctttataacg gcatatcaca ggagttccac cagcaagttt taccatttca ggataactta
      301 cccagtaagg tgcaggaata attacctggt ccccttcatc acaaagggca aagagcacat
      361 tgaacaaact atgttttgcc ccgcatgaga caacaatctg gtctggttca taagcaaggt
      421 tattatctcg tttgaatttt tcacagattg cctgtctcag tggtttaact cccgatgcat
      481 ctgtatactt tgtaaaccct tcattcaatg cgttgatggc agatttttta ataaaatcag
      541 gtgtatcaaa atcaggttct ccaacagaaa gattgataat tttttctccg gttgccacaa
      601 gctccattgc cttcttattt accgcaagtg tgcttgatgg cttgatgttt tttgcctttt
      661 gcgaaatttt tttctcaatc atgtttttcc tttttggcaa taattctacc ataaaaagtt
      721 tgcaaattca atggtttgaa attaatatat tttcttgaca tttttcgttt ttgcgtttta
      781 atatgtaaac agggtttaca aaaaggggat tcaatgacgt gcatcgcagt catcaatcag
      841 aagggcgggg tcgggaaaac cacatctgtt gtcaatttag gcgctggatt aagtttactc
      901 gggaaaaaag tgctgatgat agaccttgac ccgcagtcac atcttaccta cagcgttggc
      961 atcaaggcgc acgaactttc aaagacggtg taccatgttt ttaggtcaga cacttctgtt
     1021 tctgatgcaa ttgttgaaag gcaaataagt gtgcatcttg catttcccga aataaaagaa
     1081 acaacgggtg tattttttat tcttccttct tctcttgatc tttctggcgc tgaatttgaa
     1141 tttggcggta ttgcaggaag ggaatttcta ttgaaggagt gtttatctga agcaggcgat
     1201 tttgactatg ttttcattga ttgtccgccg agtctgggac ttttgacttt gaacgctctt
     1261 acgaccgctg atgaaatcta tattcctctt caaactgaat ttcttgctct tcagggaatc
     1321 agcaagctgc ttgaaactgt tgatgttgtt caaaagcggc tcaatcagcg aataaaactt
     1381 acgggtatta taggaacaag atttgatggc aggaaaaggt taaacagaga ggtggttgaa
     1441 aaaatcaagg attattttgg aggtcgtgtt ttcaggacac tgataagaga aaacatttct
     1501 cttgcagaag cgcctggttt tgggaaaacg atttatgagt ataagcccga tagttacggc
     1561 tcagaagatt actttcatct gtgtcttgaa atcatcgaaa gaggtaagaa acattaatat
     1621 ggaggaaatt gtatggcgga aaaaaaactt actcggctcg gttctgatcc tttgagatgg
     1681 atacaggata gtagaaaaca ggaaacagca gattcatccg ggaaaaagaa aatccagcag
     1741 aaacagggag tcccaaaaac taaggaaggt ttgaaagagg gctggattcg agcaactttt
     1801 attgtcagag aagaacatct tgaaaaaatc aaagcggttt cttactggga tagaaaagat
     1861 attaaacagg ttgttgacga agcacttggg gagtatctta aaaacaaacg ggtaaaaccc
     1921 agaccttaaa aactgaaagg aaaagatgtc tgacacaaga aaacttcctg taacaatcaa
     1981 gaactttttc tcaaaaatca atgaaagtgt cacaaaagca agaaattcag aacatctgct
     2041 taccctgatt tcaaaaattg ctgtggacga atttgacgct gacagggcat caatttttct
     2101 gcgagatgaa aaaacagatg agctccgtct tattgctggc acaggaattt ctgaagaaat
     2161 tgttcggagt catcctgttg cccagcagca gaatatcagt tactgggttg cagaaaacaa
     2221 aaagcccatc attcttaagg gttctgtgaa aaaagacaaa cgttttcaat ctcagagtac
     2281 gaccgttatt gcgtcttcaa taatagtacc attgattttt gaaaattctg ttgtgggtgt
     2341 ttttaatctg tcccgtttgg ctgagggcag acctgatttt actgaagaag acatgacact
     2401 tttcaggttt ctcggagacc ttgtggtaat atcactgcag ttgcttatca ctcaggaaaa
     2461 aaggattcac agcgaacaac ttgccgcaat tggacttgca acagcagaac tggtgcattc
     2521 attaaaaaat cttcttgtcg gcattttcgg agcagcagaa ctggttgatg ttctgatgca
     2581 gcaaaatgac tgggattcag taaaggaaaa ctggtcactt cttgctgaca acattaaaaa
     2641 tatctcaacg gttgtaaacg aggttctttc atacagtaaa tcaagttcaa tcacaaaaca
     2701 ggaacttgat atcagggaaa ttgttgaatc cttatgtaat tttatcaagc caaggtgcaa
     2761 catatcgaat attcaactgt tttttcagtg tggcgatgaa aaaattttag tgtggggaaa
     2821 cagggaatct ctctataacg ccttgatgaa cattttagac aatgccatta gagcaatgcc
     2881 agacggagga aaactgaata tatcttgcat gattgaagat gatatggtaa gaatagaaat
     2941 atctgacaca ggagttggta taccagaaga aaatctcgaa aaaatttttg agccattttt
     3001 tacaaccgac aaaaagaaag gtactggaat tgggcttgcg ctgacaaaaa aa
//
LOCUS       DAORYU010000180         3007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_21803_length_3007_cov_3.403455, whole genome shotgun sequence.
ACCESSION   DAORYU010000180 DAORYU010000000
VERSION     DAORYU010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21803_length_3007_cov_3.403455"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1989)
                     /locus_tag="PLW07_05880"
     CDS             complement(<1..1989)
                     /locus_tag="PLW07_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50090.1"
                     /translation="MDRKLQCFLMLCAGFFLLSSTLKAAPPAAPGDLEHTQNTTSAIF
                     WRWDDNSNNEQGFKCYEWYYQQVGSEWHFLLSKDPVWSVNANISSYIETGLAPNTQYR
                     RVIQAWNTDGISPSYWESHRVAYYTSIEPPAGLTFGPKGFDFWSKMARLTVKLTPPLP
                     SNLTTTDSVSPPRYGYFTPNDSGFYIEEITTGINIANTTFNTEGVIKFDPAPPTSDGW
                     WKYNNDYDPLPSMSGTREDVRFEHYLKNGLLTKSGTTYTCNHKWDYWCVENLEPNTPY
                     RFRAKARNGDKDETAWCNTTEEIWTLPYNPEISYDREIGVCYNMGTKFTFSSMIPFGI
                     GWVDHYHIKWTTNIGDMPTEEDTRWETGNWVITENRGGDWYLIVISHNKLNEISGDYN
                     YSGQFEPFCLTLGPFKVGYDVKGKVTISGGTGSYTDVVVHCGGNTTNCDSYGRFEFLH
                     LPPGTYDVYTELPGYRIAYPIENSGHYSIKIPDNADAPTFIKKGVDFTLAYKNTYTIA
                     GKVTITGGPGVAPSDVTITCGSYTPVHPDSNWNYFVPGVFAGTYKVKATITSFDYEIT
                     FPSGFEYTVSVGPDATGKDFVFTYIPGTDVASISGTVKLIGGTGDPAKAIVYCKNLNS
                     GITGQANPDHTGKYSFSNLPKGKDYQLWVKMDGYKLSRV"
     gene            complement(1989..2678)
                     /locus_tag="PLW07_05885"
     CDS             complement(1989..2678)
                     /locus_tag="PLW07_05885"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOL50091.1"
                     /translation="MTKREVEIVCGSCYHVFNSAINGTKVFQQQAHYERFVRLIRYYR
                     KNMFWKYARFECLKTYSPEIVLSQTNAEKLVDVIAYCIMPDHFHIILKQVLDGGVSKF
                     MAKITNAYAKFFNRIKKKNGPVWSGRYRCSKIETEKEFLCISRHLHMEPVFAGLVKKP
                     EQYEYSSFREYINRVKNDDTKICSFDGYVNIEPKEYATFVKDTDNYIKTFDTISRLIF
                     ENCLIPQTRRI"
     gene            complement(2694..>3007)
                     /locus_tag="PLW07_05890"
     CDS             complement(2694..>3007)
                     /locus_tag="PLW07_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50092.1"
                     /translation="CNAGDTIRVRIDIVPANQTSGIIITEKIPDGWKILNSEPAFTKV
                     LPDNSYKWLQWAKSLTSFTIIYQLKVPESATGTYTITGILTTYREGDIEIIGKKEIVV
                     K"
BASE COUNT          839 a          629 c          567 g          972 t
ORIGIN      
        1 tactcgactt aacttgtagc catccatctt cacccagagt tgataatctt tgccctttgg
       61 aagatttgaa aacgagtatt ttcctgtatg atcaggattt gcctgaccag taatgcctga
      121 attaagattt ttgcaataaa caattgcctt tgccgggtca cctgtgccac caattaattt
      181 cactgttcct gaaatgcttg caacatcagt accaggaata taggtaaaaa caaaatcttt
      241 tcctgtagca tcaggtccca cagaaacagt atattcgaaa ccagatggaa aagtaatttc
      301 ataatcaaaa ctggtgatgg tcgcctttac tttatatgtt ccggcaaaaa cacctggaac
      361 aaagtaattc cagtttgaat caggatgaac tggagtataa cttccacagg taatcgtaac
      421 atctgaaggg gcaactccag gaccacctgt aattgttact tttcctgcaa tggtataagt
      481 atttttgtaa gcaagggtga aatcaacacc tttttttatg aatgttggtg cgtcggcatt
      541 atctggaatt ttgattgaat aatgtccact gttctcaata ggatacgcaa ttctgtaacc
      601 aggtagttca gtatacacat catatgtgcc tggaggcagg tgtaaaaatt caaaccttcc
      661 atatgaatca caatttgtcg tatttccgcc gcaatggaca accacatccg tatatgaacc
      721 tgttccacca ctgattgtta ccttaccttt gacatcatag ccaaccttaa aaggtcccaa
      781 ggtcaagcaa aagggttcaa attgacccga ataattatag tcgccactga tttcattgag
      841 tttattgtgc gagataacaa taagatacca atctcccccc ctgttttcag taataaccca
      901 gttccctgtc tcccagcgtg tatcctcctc tgtgggcatg tcacctatat ttgttgtcca
      961 cttaatatga taatggtcaa cccagccaat accaaaagga atcatgcttg aaaaggtaaa
     1021 ttttgttccc atgttgtaac aaacacctat ctctctgtcg taactgattt ccgggttgta
     1081 tggaagagtc cagatttcct ctgttgtatt gcaccatgcg gtctcgtctt tatccccatt
     1141 tcttgctttt gccctgaatc tgtaaggtgt gttcggctcg aggttttcaa cacaccagta
     1201 gtcccatttg tgattgcatg tgtatgtggt cccgcttttt gttagtaaac cgtttttcag
     1261 gtagtgttca aagcgtacgt cctcacgagt gccagacatg gatggaagag ggtcataatc
     1321 attgttatat ttccaccaac catcactggt gggtggtgca ggatcaaatt taataacacc
     1381 ctctgtattg aatgttgtat tcgctatgtt tattcctgtg gtaatctctt caatataaaa
     1441 tcctgaatcg tttggcgtaa aataaccata ccggggaggt gaaaccgaat cagtagttgt
     1501 aaggtttgaa ggaagcggtg gcgttaattt gacggtaaga cgggccattt tagaccagaa
     1561 atcaaatccc tttggaccga aagtgagacc tgctggaggt tcaattgatg tataatatgc
     1621 taccctgtga gattcccagt aacttggtga gataccatca gtattccatg cctgaataac
     1681 ccttctgtac tgggtattcg gcgcaagacc tgtttcaatg taagacgata tatttgcgtt
     1741 cactgaccag actggatctt tgctcaaaag aaagtgccac tcactcccaa cttgctgata
     1801 ataccactca tagcatttga atccctgttc attatttgaa ttgtcgtccc atcgccagaa
     1861 aatggcgctg gtagtgttct gggtgtgctc aaggtctcct ggcgctgctg gtggtgctgc
     1921 tttcaaagtg ctggacagca ggaaaaatcc agcacacagc atcaaaaaac attgtagttt
     1981 tctatccatc atatcctccg tgtttgtggt attaaacaat tttcaaaaat tagacggctg
     2041 attgtgtcaa aggttttaat gtaattgtct gtgtctttaa caaatgtcgc atattctttt
     2101 ggttctatgt taacatagcc atcaaaagaa cagatcttcg tatcgtcatt ttttactctg
     2161 ttgatgtact ccctgaatga actgtactcg tactgctcag gtttttttac gagccctgca
     2221 aaaactggtt ccatgtgaag atgccgagaa atgcaaagaa attctttttc tgtctcaatt
     2281 ttagaacacc tgtatcttcc tgaccagaca ggaccatttt tcttttttat tctgttaaag
     2341 aactttgcat aggcattcgt aatttttgcc ataaattttg atactccacc atcaagaacc
     2401 tgtttcaata taatatgaaa atggtcaggc ataatacaat atgcaatgac atcaacaagt
     2461 ttttctgcat ttgtctgaga cagcacaatc tcaggcgaat aagttttcaa acactcaaat
     2521 cttgcatatt tccagaacat attttttctg taataccgta ttaacctgac aaatctctca
     2581 taatgcgcct gttgttgaaa aacttttgtg ccattgatag cactgttaaa tacatggtaa
     2641 cagcttccac atacaatctc aacttccctt ttcgtcatta ccagtttctg ttgttatttc
     2701 accactattt cttttttgcc gattatctca atatctcctt cacgataggt ggtaagaatg
     2761 ccagtgatgg tgtatgtgcc tgttgcagat tcaggaactt tcagttgata tatgattgta
     2821 aaagaagtaa gagatttcgc ccattgaagc catttgtaag aattgtcagg taacaccttt
     2881 gtaaatgctg gctcagagtt caatattttc cagccatcag gaattttttc agtgataata
     2941 ataccgctgg tttgatttgc aggaacaatg tcaattctca ctctgattgt atctcctgcg
     3001 ttgcagg
//
LOCUS       DAORYU010000181         2954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_22274_length_2954_cov_5.767506, whole genome shotgun sequence.
ACCESSION   DAORYU010000181 DAORYU010000000
VERSION     DAORYU010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22274_length_2954_cov_5.767506"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2050)
                     /gene="mgtA"
                     /locus_tag="PLW07_05895"
     CDS             complement(<1..2050)
                     /gene="mgtA"
                     /locus_tag="PLW07_05895"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HOL50093.1"
                     /translation="MPRLPESGIVEFVWLTQEQILSRLNTLIKGLSDEEAELRLQEYG
                     FNELFRPKKHSIIFQIASKFLNPLVIVLLIIAIFSIIFGEIASAVLVILMAIGSVSLS
                     FFQEHRAGKEAEKLSEMVRPTATVYRNGVPKEIPIREIVPGDIVYLSAGDMVPADLRI
                     ISCKDLFVNQASLTGESFPVEKTAAPVITTNHQPSELKNIAFMGSSIVSGTGLGVVIK
                     TGLSTQFGMVSYKMATLSAETNFDRGVRSFTWLMIRFMLVLVVIIFFINAMTKGDFLQ
                     SFLFALAVSVGLTPEMLPMLVAVNLSKGAISMSRKDVIVKQLSSIQNFGAMDVLCTDK
                     TGTLTLDEIILEIYCDVTRQENEDVLQFAYINSYYQTGLKNILDRAVLKYRELSIQQY
                     RKIDEIPFDFSRKMMSVVVEIDGKHRLITKGAPEEVFKKCVKYELDGEIFDIDPLVLT
                     DLKLEYEHLSAQGFRVLAIAYKDYEIPKEAYTKNDEVEMILKGYVAFLDPPKPTAEET
                     IQALRKRGIELKVLTGDNELVTRHICNEVGLDVRNMLTGEQIEMMSDKDLIEVVKNVT
                     VFSRLSPLQKERIIRALHKNGHTVGYLGDGINDALALKAADVGISVDNAVDIAKESAD
                     IILLQKSLTVLEDGVIEGRKTFGNILKYIKMGSSSNFGNMFSMTGASIFLPFLPMLPI
                     Q"
     gene            2276..>2954
                     /locus_tag="PLW07_05900"
     CDS             2276..>2954
                     /locus_tag="PLW07_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HOL50094.1"
                     /translation="MLVSIGSIIIAFLFALEILRKDAGNEKMQEIANAIKEGAGAFLK
                     RQYTTITVLSITLAIIIFLIYALSGKITYGWHTSLAFILGALCSSISGYVGMYISVRS
                     NSRAASAAGRSMNEALVTALKGGAVSGILVVALSLLGVAGIFLLYGGSANPLEVPLLI
                     VGFGFGASFVALFAQLGGGIYTKAADVGADLVGKVEAGIPEDDPRNPAVIADLVGDNV
                     GDCAGRGA"
BASE COUNT          851 a          629 c          551 g          923 t
ORIGIN      
        1 tctgaattgg aagcattggc aggaatggca gaaatatgct tgcgcctgtc atactgaaca
       61 tattaccaaa gttcgaactc gaacccattt tgatatattt caaaatattt ccgaatgttt
      121 ttcttccttc gatcacgccg tcttcaagaa ccgttaagct tttctgcaga agaataatgt
      181 ctgctgattc ttttgcgata tccacagcat tatcaactga aattccaaca tcagctgcct
      241 ttaaagccaa agcgtcattg atgccatctc caagatagcc cactgtatga ccatttttat
      301 gtaatgctct gataattctt tctttttgca atggcgacaa tcttgaaaat acagtcacat
      361 tttttacaac ctcaatcagg tctttatcac tcatcatttc tatctgctca ccagtcaaca
      421 tgtttcttac atcaagccca acttcattac atatgtgcct ggtaaccagt tcattgtcac
      481 cagttaacac ctttaattca attccccttt ttcttagtgc ctgaattgtt tcctctgctg
      541 ttggcttggg tgggtcaaga aatgcaacat aacctttgag aatcatttca acttcatcgt
      601 tttttgtgta tgcttctttt ggtatttcat aatctttgta agcaatcgca agaaccctga
      661 acccctgagc actcaaatgt tcatattcaa gcttcaggtc agtaagaaca aggggatcga
      721 tgtcaaatat ttctccgtcc aattcatact ttacacattt tttgaaaact tcttctggag
      781 cacctttcgt aatcagtcgg tgtttgccat cgatttccac gacaaccgac atcatcttcc
      841 ttgaaaaatc aaatggaatt tcatcaattt ttctgtactg ctgaatcgac aattctctat
      901 atttcaacac cgctctatca aggatgtttt ttaatccggt ctggtaataa ctgttaatat
      961 aggcaaattg taatacatct tcgttctcct gtcttgtcac atcacaataa atttcaagaa
     1021 tgatttcatc aagggttaat gtgccggttt tatctgtaca cagtacatcc attgcaccga
     1081 aattttgaat agagctcaac tgttttacaa tgacatcctt tctagacatg gagatagcac
     1141 cctttgaaag gttaacagcc acaagcatcg gaagcatttc aggggtaagc ccaacagaaa
     1201 cagcaagagc aaaaagaaag gattgcagaa aatcgccctt tgtcatcgca ttgataaaaa
     1261 atataatcac aaccaggaca agcataaacc tgatcatcaa ccaggtaaaa gaacgcactc
     1321 ctctatcaaa atttgtttca gctgacagtg ttgccatttt gtaggaaacc attccgaatt
     1381 gagtggacaa tcctgtttta attacaactc caagacctgt accgctaaca atacttgaac
     1441 ccataaacgc gatatttttc agctctgacg gttgatgatt tgtagtaata acaggtgctg
     1501 cggttttttc aacaggaaag gactcacctg tcagtgacgc ctgattaaca aacaaatctt
     1561 tacaggaaat aattcttaaa tcagcaggaa ccatgtcccc agcagaaaga tatacaatat
     1621 caccaggaac gatttctctt attggtattt ctttcgggac gccatttcta taaacagtag
     1681 cagttggcct gaccatttca ctgagttttt ctgcttcttt tccagcgcga tgttcctgaa
     1741 aaaatgatag agaaacactc cctatggcca tcaagattac cagtaccgca ctggctattt
     1801 ccccaaaaat aatggagaaa attgcaataa tcagtaaaac gatgaccaaa gggttcaaga
     1861 actttgaagc aatttgaaat ataattgagt gcttcttggg tctgaaaagc tcattgaagc
     1921 catactcctg aagcctcagc tctgcttctt catcgctcag accctttatt aatgtgttga
     1981 gtcgtgataa aatctgttcc tgcgtgagcc aaacaaattc aactattcca gattctggta
     2041 atcgcggcat tattcaatcc tctgcaaaat gtttcgtgtt tagattgata attattataa
     2101 cctgttaata gcatactaaa aagtttctgc tcgtttgtgc aattctatgt tagtatccat
     2161 cacaggggct tacaaatggt agacacgtca aaaattcatt tgaaataatt ttttttgggt
     2221 gttaaaataa tgcgattttt tcaacatgga ggcgaatatg tatttcaatc tggtaatgct
     2281 ggtcagtatt ggaagtatca tcattgcttt cctgttcgct cttgaaattt taaggaagga
     2341 cgcaggaaac gaaaagatgc aggaaattgc aaatgcaata aaagagggtg caggcgcctt
     2401 tctcaaaagg cagtacacaa caataacggt tctttcaatc acactggcaa tcatcatatt
     2461 tctcatttac gcactcagtg gaaaaatcac atatggatgg catacgtctc ttgcctttat
     2521 tctcggcgca ctttgctcat caatttcagg ttatgtggga atgtacatat ctgtcaggtc
     2581 aaactctcgc gctgcgtctg cagcagggcg aagtatgaac gaagcacttg tcactgcctt
     2641 aaagggcggc gctgtttcag gcattcttgt tgtggctctt tcacttcttg gcgtggctgg
     2701 catatttctt ctttatggag gaagtgccaa tccacttgaa gtgccacttc tgatcgttgg
     2761 ttttggtttt ggtgcatcat ttgtggcact tttcgcgcaa cttggtggcg gtatctatac
     2821 aaaagctgct gatgtgggtg ctgaccttgt tggaaaagtt gaggcaggaa tacctgaaga
     2881 tgacccgaga aatccagcag tcattgctga ccttgttgga gacaatgttg gcgattgcgc
     2941 cggccgtgga gcag
//
LOCUS       DAORYU010000182         2940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_22418_length_2940_cov_4.344541, whole genome shotgun sequence.
ACCESSION   DAORYU010000182 DAORYU010000000
VERSION     DAORYU010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22418_length_2940_cov_4.344541"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1668
                     /locus_tag="PLW07_05905"
     CDS             <1..1668
                     /locus_tag="PLW07_05905"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOL50095.1"
                     /translation="TYNLIFFIMLPVSIVVLRKKLSFSYSQFCFLIFSILCLLFIPLW
                     PVWTTTIKQFSYLFGLFATWFLFKMVFDEKEIPYVTSVFLCSMILIIVYGIHQYFWGL
                     EATRQTISQHPELINALSETYLDRIASNRIFSTFVYPNTLAGYLLMLYPVVFFYLFSQ
                     TGIQARILAGIVLIILLPVLAATESMGGWFCFIIVSFLMLMYLVLRGKYYIYFCFLFL
                     IVFIFLAYTGFTGGLIPKFGSLADRINYWISGMQIFKKYPLIGIGPGNFSQFYLQFKI
                     PGAMEAKFAHNLIVEILVCTGLIGFLTFLTALVFFIRGNIQNFLFSKNQLVVGYFFGI
                     TGIFLHCLVDFDYANAAIICILFSFAGLVELSSNIKNIKSKTLTKLLAGIIIILMPVA
                     AVVEYKTWQVERIIENIKSGKSGNPIQVLEQASEMFPEPEIFFIEGEIFRFAYQETRN
                     SEFAERSVASYKKAIGMNPFVPRYHRMLARLFVKLGENKEAEKEFLKVIELYPTKALY
                     NIEIGEFYKKTGNEELAKIYIEKGIKLPPSSKDEILLIEEYKNGKGL"
     gene            1652..2314
                     /gene="ricT"
                     /locus_tag="PLW07_05910"
     CDS             1652..2314
                     /gene="ricT"
                     /locus_tag="PLW07_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOL50096.1"
                     /translation="MEKVFEIQVRKVEKPCYVSGDIPDLMAGDICIVEFAKGTQDYAK
                     VCTIFNLGEKDEKQTISGKILRKANEQDMLVIRENEILKEEAFKLCDGKIKEINLPMK
                     LIDVTYSFDRTVITFYYWAAGRVDFRKLVKELASIFNCRIEMRQIGLRDEAKIKGGFG
                     ICGQEICCHRFLKDFEPVTMKLVKTQSLPLDMSKISGLCGRLMCCLIFESKMYENGED
                     EQ"
     gene            2311..>2940
                     /locus_tag="PLW07_05915"
     CDS             2311..>2940
                     /locus_tag="PLW07_05915"
                     /inference="COORDINATES: protein motif:HMM:NF020892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOL50097.1"
                     /translation="MKFYVTTPIYYINAQPHIGHCYTTIAADCLARYHRMKGKDVFFL
                     TGTDEHGDKIAQAAEKKGVTIEQFTDEMSDMFKKIWNTLNISYTRFIRTTEKEHQHNV
                     SEVFKKLMENGDIYQGEYSGYYCVPCECFIPKTKIDQSNPICPDCNRSVKILTEKSYF
                     FRLSKYQQALINYFEKNPDMIQPQFRLEEVKSFISQGLNDLSVTRQGSIW"
BASE COUNT          967 a          460 c          563 g          950 t
ORIGIN      
        1 acatataatc tcatattttt cattatgtta cctgtttcaa ttgttgtgct cagaaaaaaa
       61 ctgagttttt catattctca attttgtttc ttgatttttt ccatattatg tctcttattc
      121 attcctttat ggcctgtatg gacaacaaca ataaaacagt tttcttatct ttttggactt
      181 tttgcaacat ggtttctttt caaaatggtc tttgacgaaa aggaaatccc ttatgtgacc
      241 agtgtatttc tctgttcgat gatattgatt atcgtttatg gcattcatca gtacttctgg
      301 ggactggaag ccacacgaca gacgatatct cagcatccag aattgataaa cgctttgtct
      361 gaaacatacc tggatagaat tgcaagcaac aggatttttt caacctttgt ttatcccaat
      421 acactggccg gatatctgtt aatgctttat cctgttgtat ttttttatct tttttctcaa
      481 acaggaatcc aggcacgaat actggcagga atcgttctta tcatattatt accggtactg
      541 gcagctacag aatccatggg tggatggttc tgctttatta ttgtttcatt tctgatgcta
      601 atgtatctgg tgcttcgggg gaaatattat atctattttt gtttcctttt tttgatagta
      661 tttatttttc ttgcgtatac tggatttaca ggtggtttga taccaaaatt tggttctctt
      721 gcagacagaa taaattactg gatctcaggt atgcaaatat tcaaaaaata tcctttaata
      781 ggtattggac ctggaaattt ttctcaattt tatcttcagt tcaaaattcc tggtgcaatg
      841 gaagcaaaat ttgcacataa tttaattgtt gaaattcttg tgtgcacagg attgattgga
      901 tttttgactt ttttaacggc gttggttttt ttcataagag gaaatattca gaattttctt
      961 ttttcaaaaa atcaattagt ggtcggttat ttctttggaa taactggaat ttttctccac
     1021 tgccttgttg attttgatta tgctaatgca gcaattatct gtatattgtt ttcctttgca
     1081 ggacttgtag aattatcttc gaacataaaa aatataaaat caaaaacatt gacaaaattg
     1141 cttgctggca ttatcattat tctgatgcct gtggcggcag ttgtggaata taaaacgtgg
     1201 caggtggaaa ggattataga aaacatcaaa agcggtaaaa gcggcaatcc cattcaggtt
     1261 ctggaacagg catcagagat gttccctgaa cctgaaatat tttttattga gggagaaatt
     1321 ttcagattcg cttatcagga aaccagaaat tcagaatttg ctgaacgatc tgttgcatct
     1381 tataaaaaag ccatagggat gaatcctttt gtaccgcgct atcacagaat gcttgccagg
     1441 ttatttgtga aacttgggga aaataaagaa gcagaaaaag agtttttaaa agtaatagaa
     1501 ctttatccga caaaagcact ttataatatt gagattggtg agttttataa aaaaacaggg
     1561 aacgaggaac ttgcaaaaat ttatattgaa aaaggaataa aattaccccc tagcagcaaa
     1621 gatgaaatac tcttaattga ggagtacaaa aatggaaaag gtctttgaaa ttcaggtcag
     1681 aaaagtggaa aaaccatgct atgtttctgg agatatccct gacctgatgg caggagatat
     1741 ttgtattgtt gaatttgcca aaggaacaca ggattatgca aaagtctgta ctatttttaa
     1801 cctcggagag aaggatgaaa aacagaccat ttctggaaaa attctcagaa aggcaaacga
     1861 acaggacatg ctggtaattc gggaaaatga aattcttaaa gaggaagcat tcaaactctg
     1921 tgatggaaaa ataaaggaaa ttaatctgcc aatgaaactt attgatgtta cctattcatt
     1981 tgatagaaca gtaataacat tttattactg ggctgctgga agggttgact tcagaaaact
     2041 tgtaaaggaa cttgcatcta ttttcaactg cagaattgag atgcgtcaga tagggctgag
     2101 agacgaagca aaaataaaag gtgggtttgg catatgtggg caggaaatat gctgtcatag
     2161 attccttaag gactttgaac ctgtcacaat gaaactggta aaaacacaaa gtcttccgct
     2221 tgatatgagc aaaatttccg gtctgtgcgg aaggttaatg tgttgtttga tatttgaaag
     2281 caaaatgtat gaaaatggag aagatgaaca atgaaattct acgtcaccac gcccatttat
     2341 tatattaacg ctcaaccaca tattggccat tgctatacaa caattgctgc tgattgtctt
     2401 gcaagatatc accgaatgaa aggaaaggat gtgttttttc tcacagggac agatgaacac
     2461 ggagataaaa tagcccaggc ggctgaaaaa aagggtgtta caattgaaca gttcacagat
     2521 gaaatgtctg atatgtttaa aaaaatatgg aacacactta acatttctta tacaagattt
     2581 atacgtacga cagaaaaaga acatcagcat aatgtaagcg aagtatttaa gaaactgatg
     2641 gaaaatggtg acatatatca gggagaatac tccgggtatt attgcgttcc atgtgaatgt
     2701 ttcataccga aaacaaaaat tgaccaatca aatccaatct gtcctgattg taataggtct
     2761 gtaaaaatac ttactgaaaa atcatatttt tttagattgt ccaaatatca acaggcactc
     2821 atcaattact ttgaaaaaaa tcctgacatg attcaaccac agttccgtct tgaagaagtc
     2881 aaaagtttta tatctcaggg actcaatgac ctttctgtca caaggcaggg ttctatatgg
//
LOCUS       DAORYU010000183         2935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_22478_length_2935_cov_3.400000, whole genome shotgun sequence.
ACCESSION   DAORYU010000183 DAORYU010000000
VERSION     DAORYU010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22478_length_2935_cov_3.400000"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="PLW07_05920"
     CDS             complement(<1..769)
                     /locus_tag="PLW07_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50098.1"
                     /translation="MNIAFEGTTWRKQLAIDIINRLGIIFNQTTSENADIIIVCDYHH
                     ISSEEMLHLVKNRKNVILLQPEKNIVNIFGCELMYTYHFPLMQIDEHLPGRFVQVFPP
                     TLLIKQKHGNISGYFAFDFSCSGENYTTKYPAVSWREIGNGSICMFFYDFISTILLLL
                     QGWEFFTEEGKLSRRFRTGISRATHLADHLINKELCEIPQAYCHEILLLNLIRTMLWK
                     KNPVPRIWYYPYPATTVFLLSGDSDNLEKHNLEKAWEL"
     gene            883..2178
                     /locus_tag="PLW07_05925"
     CDS             883..2178
                     /locus_tag="PLW07_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50099.1"
                     /translation="MRLTNGILCFENGLKKNIDIAIKNEKIYLEKGNKKNDEKVIDLK
                     GKYVFPGFIELHTHGAGLFSFYAGRYNIKKQRFEHSSEIYQEGLTQWAHLRAKTGATR
                     LYASTAAIPIEKIKFALRELETFIRNNKNNWEGKFYAGAMIEGTFINRANQGAMNPEY
                     AFEPEKSVFDEINETGLARLVNVPPDHGKKSVKLTEYLVSKGISVGAGHTSATYQQFK
                     EAVGAGLKYFVHFLNGPTGNLYKSFFGGGALEAALTLDIMLELIVDGYHVAPWYIRET
                     FARKEPSDIMAITDAVFASQAKGIKQFECDGIGGTVDSKRQYVYVTKVGPTTLYGSMV
                     TIDRVFSNLLSYLTKQMPGIWHKNHPALNFEKALCITSSCCSTNIVRMIKKNNGDDLN
                     TGELKNGKLADLVIGEIKGKPGDYSLKIDSVFIRGQKVV"
     gene            2203..>2935
                     /locus_tag="PLW07_05930"
     CDS             2203..>2935
                     /locus_tag="PLW07_05930"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOL50100.1"
                     /translation="MEDIKLAAISMISNPANPEKNIERHVKWIEKAVKHNVDWCLFPE
                     MSITGFCFDRNFFQASEPLNGPSTQKMIEIAKSFNITIGFGIAEKDQSDLIRNTYVFV
                     SPEGLLGRYRKTHIPPLEYTIETSGDEFTVIKTRKAKVGVNICFDNWFCESGRMSYLN
                     GAEIIVAPFYMCWETDIKTNPEKSYMAWRELAMINFRAVAWQNGLYHITINSCGGVNE
                     KGVEYYGPPLVIIINPFGEIEAETPA"
BASE COUNT          985 a          542 c          576 g          832 t
ORIGIN      
        1 taagttccca tgccttctca agattatgtt tttcaaggtt atcactgtct ccgcttaaaa
       61 gaaaaactgt tgtggcagga tagggataat accatatcct tggtactgga ttttttttcc
      121 acagcatcgt tctgataagg ttcaaaagca gtatttcatg acaatatgcc tgcggaattt
      181 cacagagttc tttgtttatt aaatgatcag ccagatgtgt agcccgtgaa attccggttc
      241 tgaaacgtct ggaaagtttc ccttcttctg taaaaaattc ccatccctga agaagaagca
      301 atatggtgct gataaaatca taaaaaaaca tacagatgct tccatttcca atttcccgcc
      361 atgatactgc agggtatttt gtggtgtaat tttctccaga gcaggaaaaa tcaaatgcga
      421 aataacctga gatgttccca tgtttctgtt taattaataa agttggagga aaaacttgaa
      481 caaatcttcc aggtaaatgt tcatcaatct gcatcaatgg aaaatgatat gtgtacatca
      541 attcacaccc aaaaatgtta acaatatttt tttctggctg aagcaaaatg acatttttcc
      601 tgttttttac gagatgaagc atttcttcag atgaaatatg atgataatca caaacaataa
      661 taatgtctgc attttcactg gtggtctgat tgaaaataat tccaagtcga ttgatgatat
      721 caatcgcaag ctgtttcctc catgttgttc cttcaaacgc aatattcata tttcctccac
      781 taaaaattcc cttcttattt ttaacccatt tgcctgaaat gttatactat taaaatagct
      841 ggcaatcaag aattttcaat atcagataaa gggagacaga acatgagact gacaaatggt
      901 attttatgtt ttgaaaacgg actgaaaaaa aacattgaca ttgccatcaa aaatgaaaaa
      961 atttatcttg aaaagggtaa caaaaaaaat gatgaaaaag ttattgattt gaagggaaaa
     1021 tatgtgtttc cgggtttcat tgaactccat acgcatggcg caggactttt ttctttttat
     1081 gctggtagat acaatatcaa aaaacaacgg tttgaacact ccagcgaaat atatcaggaa
     1141 ggattgacac agtgggcaca ccttcgtgca aaaacaggcg caacacgact ctatgcttca
     1201 actgccgcta tcccgattga aaaaattaag tttgccctca gggaacttga aacattcatc
     1261 agaaacaaca aaaacaactg ggaaggaaaa ttttatgctg gagccatgat tgagggaact
     1321 tttatcaatc gcgcaaatca gggcgcaatg aatccagaat atgcgtttga acccgaaaaa
     1381 tctgtttttg atgagatcaa tgaaaccggt cttgcgcgac tggtcaatgt gccacctgac
     1441 catggaaaaa aatcagtcaa actcactgaa taccttgttt caaaaggtat ttcagttggc
     1501 gcgggtcata catctgcgac atatcagcaa tttaaggaag cagttggcgc tggcttaaag
     1561 tatttcgtgc actttctcaa tggcccaacc ggtaacctgt acaagagttt ctttggtgga
     1621 ggcgcacttg aagcggcatt gacacttgac attatgcttg aactcatcgt ggatggatac
     1681 catgtcgctc cctggtacat cagagaaaca tttgcaagaa aagaaccatc agatattatg
     1741 gcaatcactg atgctgtttt tgcaagtcag gcaaagggaa tcaaacagtt tgaatgcgat
     1801 gggattggtg ggactgtgga cagcaaacgc cagtatgttt atgttacaaa ggtaggtccc
     1861 acaactctct atggaagtat ggtaacaatt gacagggtct tttcaaatct gttgtcctat
     1921 cttacaaaac aaatgccagg tatctggcac aaaaatcatc cggccttgaa ttttgaaaaa
     1981 gccctgtgta ttacttcatc ctgctgttca acaaatatcg tccgtatgat taagaaaaac
     2041 aatggagatg accttaacac tggtgaattg aaaaacggaa aattggctga tttagtcatc
     2101 ggagaaataa aaggaaaacc aggagattac tcattaaaaa ttgattctgt atttatccgg
     2161 ggacaaaagg ttgtttaaat taaaatatat gggagacatt atatggaaga tattaaactt
     2221 gctgccatct caatgatcag taatcccgca aatccagaaa agaacataga acgacatgtg
     2281 aaatggatag aaaaagcagt aaaacacaat gttgactggt gtctttttcc tgaaatgagt
     2341 atcactggct tttgttttga caggaatttt ttccaggcaa gcgaaccatt gaatggtccg
     2401 tcaactcaaa aaatgataga aattgcaaaa tccttcaata tcacaattgg atttggaatc
     2461 gctgaaaaag accagtcaga ccttattaga aacacgtatg tctttgtctc tcctgaagga
     2521 cttctgggga gatatcgaaa aacacacatt ccgccacttg aatataccat tgaaacatcc
     2581 ggagatgaat tcacagtaat caaaaccaga aaggcaaagg tcggggtaaa tatctgtttt
     2641 gacaactggt tctgtgaatc aggaagaatg tcctatctca atggtgcaga aattattgtt
     2701 gcaccgtttt acatgtgctg ggaaaccgac attaaaacaa atcctgaaaa atcatatatg
     2761 gcatggcgag aactcgcaat gatcaatttc agggcagtcg catggcaaaa tgggctgtac
     2821 cacattacaa ttaattcctg cggtggcgtc aatgaaaaag gggttgaata ctatggacca
     2881 ccactggtca tcattattaa tccgtttgga gaaatagaag cagaaacccc tgcag
//
LOCUS       DAORYU010000184         2905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_22790_length_2905_cov_3.426316, whole genome shotgun sequence.
ACCESSION   DAORYU010000184 DAORYU010000000
VERSION     DAORYU010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22790_length_2905_cov_3.426316"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1517
                     /gene="xylB"
                     /locus_tag="PLW07_05935"
     CDS             <1..1517
                     /gene="xylB"
                     /locus_tag="PLW07_05935"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HOL50101.1"
                     /translation="VIGIDLGTTGVKSVLVDDNGKVIASTVGQYPLITPKPTWAQQDP
                     QLWWQATVDSLKTLLKTIGAGAIIGIGLTGQMHGSVFLDEHGEVLYPAMLWCDQRTAR
                     ECDEINEIVGKDRVFEITCNPVLTGFQAPKILWLKNNHPDIFKKIKRVLLPKDYIRFK
                     LTGEFATDVSDASGTSLFDVKKRAWSNEILDALKLPLHWFPACYESVEITAKTSQETE
                     KLTGIPQGTPVVAGAGDQAAGAIGTGITKEKLISISLGTSGVVFAFSEKPFVDPCGRL
                     HTFCHAVPGKWHLMGVMLSAGGSFRWFRDTLGKQEIEQAKKENRDPYEILTSMAEQSA
                     PGSDGVIFLPYLSGERCPYPDPNAKGVFFGLSLKTTKADMVRAVLEGVSFGLRDSVEI
                     MKEIGLPFGGHCRVSGGGARSKFWAQLLADIIGEKMVRLVSEEGPAYGAALLAGSGAK
                     LFPSIEKTCDMFISTRDEFMSEPEKCRIYERIYRIYKNLYPALKPSFDLMSEIS"
     gene            1558..1950
                     /gene="rpsI"
                     /locus_tag="PLW07_05940"
     CDS             1558..1950
                     /gene="rpsI"
                     /locus_tag="PLW07_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009058059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOL50102.1"
                     /translation="MEGNLLIAVGRRKTARAVVKMSEGNGKIVINGKAIEQYFPLVTQ
                     QAEVVKPLKVTDLDGRFNIEVKSLGGGISGQVDAIKLGIARALVKYSPDLTSKLREFG
                     LLTRDPRMKERKKYGQKGARKRFQWTKR"
     gene            1990..>2905
                     /gene="argC"
                     /locus_tag="PLW07_05945"
     CDS             1990..>2905
                     /gene="argC"
                     /locus_tag="PLW07_05945"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOL50103.1"
                     /translation="MVRAGIFGISGYAGRKLVEILLLHPEVKITCGFVAPDEPEMPLS
                     QMHPRISKTIDIFCYNKVDWKLVEKNCDIVFLALPHVVSMSFVPQILSLGKKVIDLSA
                     DYRFSDALIYEKWYQKHSSPELLSQAVYGLPEINRKEIAKASLIANPGCYPTSALLGM
                     MPIAKKNLISGRVIVNSLSGFTGAGRKPDVTLLFAEGNENSRAYKIGQHRHQPEIQHI
                     LEKSGCRVDVTFVPHLIPVNCGILTTMYVNLAEDIDEKQLQQMYTGFYSNEPFVRVMD
                     YGCSPEIKNIVGTNFCDIGIRKIDSRFLV"
BASE COUNT          889 a          506 c          684 g          826 t
ORIGIN      
        1 atgtcattgg tattgacctt ggcacaacag gggtaaaaag tgttcttgtt gatgacaatg
       61 gaaaggtgat tgcttcaact gttgggcagt atcctttgat tactccaaag ccaacatggg
      121 ctcagcagga cccgcaactg tggtggcagg caacagtgga ttctttgaaa accctattga
      181 aaacgattgg tgccggcgct attattggaa ttgggctcac aggtcagatg catggttctg
      241 tgtttcttga cgaacatggt gaagtgttat atccagcgat gttgtggtgt gaccaacgta
      301 cagccagaga atgtgacgag atcaatgaaa ttgttggcaa agatagagta tttgaaatta
      361 cgtgtaatcc agtattaacg ggttttcagg caccaaaaat cctctggcta aaaaacaacc
      421 atccggatat tttcaaaaaa attaaaaggg ttcttctacc aaaggactat atcagattca
      481 aactgacagg cgagtttgcc acagatgtgt cagatgcatc aggaacaagt ttgtttgatg
      541 tgaaaaaaag agcatggtca aatgaaattc ttgatgcact taaattacca cttcactggt
      601 ttcctgcctg ttatgaatca gttgagataa cagcaaaaac ttctcaggaa acagaaaaac
      661 ttacaggaat tccacaaggt actccagtgg ttgctggtgc tggagaccag gcagcaggag
      721 ccattggtac tggcattaca aaagaaaaat tgatttctat atctctggga acaagtggag
      781 ttgtgtttgc attttctgaa aagccatttg ttgacccttg tggcagatta cacacatttt
      841 gccatgctgt gcctggaaaa tggcatttaa tgggggtaat gctttctgct ggtggttctt
      901 ttaggtggtt cagggatacg cttggaaaac aggagataga gcaggcgaaa aaagaaaaca
      961 gagatccata tgaaattctt acatcaatgg ctgagcaatc tgctcctggt tctgatggtg
     1021 tgatatttct tccgtatctg agtggagaaa gatgtccata tcctgatccc aatgcgaagg
     1081 gtgtcttttt cggactttct ttgaaaacga caaaagcaga tatggtgagg gcagtgcttg
     1141 agggggtgag ttttggactc agagacagtg ttgaaatcat gaaggaaatc gggttgcctt
     1201 ttggtgggca ttgcagagtg tctggcggtg gtgccagaag taaattctgg gctcaacttc
     1261 ttgctgatat cattggagaa aaaatggttc ggcttgtttc tgaagaagga ccggcatatg
     1321 gtgcagcact tcttgctggc tcaggggcaa aattgtttcc ttctattgag aaaacttgcg
     1381 acatgtttat ttcaaccaga gatgaattta tgtcagaacc agaaaagtgc aggatttacg
     1441 aaagaattta caggatttat aaaaatcttt accccgcgtt aaaaccgtca tttgacttaa
     1501 tgagtgagat atcttaaaat acatacctaa aaattatttt gacggaggca aaattcaatg
     1561 gaaggaaatt tactgattgc ggttggtaga agaaaaacag cccgtgctgt cgtgaaaatg
     1621 tctgaaggaa acgggaaaat cgtgattaat ggaaaagcaa tcgagcagta ttttccactt
     1681 gttacacagc aggcagaggt tgtaaaaccg ttaaaggtta ctgaccttga tggaaggttt
     1741 aatatcgaag tgaaaagcct gggcggcggc atttcgggtc aggtggacgc aataaaactg
     1801 ggaatcgcca gagcacttgt taaatacagt ccagacctca cttcaaaatt gagggaattt
     1861 gggcttctga ccagagaccc acggatgaaa gaaagaaaaa agtacggtca gaaaggtgcc
     1921 agaaaacggt tccagtggac aaaacgttaa gccagtatct atttcctgca ttttatggac
     1981 aacaaaaaaa tggtacgcgc tggaattttt ggtatatcgg gttatgctgg caggaaactt
     2041 gttgaaattc tgctattaca tcctgaagtg aaaattacct gcggatttgt tgctcctgat
     2101 gaaccagaaa tgcctctttc tcaaatgcat cccaggattt cgaaaacaat tgatattttc
     2161 tgttacaaca aggttgactg gaaacttgtt gaaaaaaatt gcgacattgt ttttctggcg
     2221 ttgccacatg ttgtttcaat gtcgtttgtt cctcaaattc tttctctcgg gaaaaaggtt
     2281 attgatttaa gtgctgatta cagattttct gacgcattga tttatgaaaa atggtatcaa
     2341 aaacattcgt ctccagaact attgagtcag gcagtgtatg gacttcctga aatcaacagg
     2401 aaggaaattg caaaagcatc actaattgca aacccaggtt gttatccaac gtctgctctt
     2461 ttagggatga tgccaattgc gaaaaaaaat ctcatcagcg gcagggtgat tgtaaactct
     2521 cttagtggtt ttacaggtgc tggaagaaaa cccgatgtta cacttctttt tgcagaaggt
     2581 aacgaaaata gtcgcgccta caaaatcggt cagcaccgcc atcaacctga aattcagcat
     2641 attcttgaaa aaagcggttg cagggtggat gtaacatttg ttcctcattt aatccctgtt
     2701 aattgtggaa ttttgacaac aatgtatgtg aatctggctg aagatattga tgaaaaacaa
     2761 ttacaacaga tgtacactgg attttattca aatgaaccct ttgtcagggt tatggattat
     2821 ggttgttcgc ctgaaattaa aaatatcgtc ggaaccaatt tctgcgatat cggcatcaga
     2881 aaaatagaca gtcgcttcct tgtga
//
LOCUS       DAORYU010000185         2894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_22909_length_2894_cov_3.232476, whole genome shotgun sequence.
ACCESSION   DAORYU010000185 DAORYU010000000
VERSION     DAORYU010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22909_length_2894_cov_3.232476"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="PLW07_05950"
     CDS             complement(<1..752)
                     /locus_tag="PLW07_05950"
                     /inference="COORDINATES: protein motif:HMM:NF024924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOL50104.1"
                     /translation="MKTYFIENNRIEELFTGWLGDYDVFAPFSRSQHLLYESVSKENF
                     DEAVIGVVRPIQPLKFFLYPFQERVVPEIATSRKRIIIGAANCDLQGLKILDRVFLEG
                     DYKDPNFIQRRQNTFIVSIDCSQPLSVCFCKSLGLNPYPEDGFDLNLTKLEGGIVVDV
                     GSEKGEVLLQDKSLLKDVSESFLEQRKKLREGVSKKIVKYNEQFHIPEKISEQFKSMF
                     GSKKWGMVWEHCVQCGSCTSVCPSCVCFLLEDV"
     gene            complement(953..1996)
                     /locus_tag="PLW07_05955"
     CDS             complement(953..1996)
                     /locus_tag="PLW07_05955"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOL50105.1"
                     /translation="MKNHPVKIGIIGLGRAGWGMHCCELDNFKDKFQIVAGCDIVQDF
                     RKRFVEKYGCKAYKRVEDLINDPDVELVDIASRSKDHVEHAIMALKANKYVFLEKPLA
                     TSYSEALKIDRLPASQKKKLFIRHNRRFDPAFQHIREIIASGILGQVFEIKLRRNNYQ
                     RRNDWQTLKKYGGGLLLNWGPHIVDHALRFLDSPVKEMWSDLKKIAAAGDAEDHIHII
                     LKGKNQMVVDLEISGGAAISESEYLVLGSKGALECTGNEIKLRYLDPSVPLRELKANP
                     GTPGVGFISPEKLVWIEKTIPVNPELKVEMHFIWDYLYEAIRNKKPFPVKWKQALEVM
                     RIISAAKKGTKFS"
     gene            complement(2016..2759)
                     /locus_tag="PLW07_05960"
     CDS             complement(2016..2759)
                     /locus_tag="PLW07_05960"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOL50106.1"
                     /translation="MKFGVADYGMNVWYGGLFDIEQRLTDLKKIGYDGTERLVASSPS
                     EALLRSGTYRKLGMDFATCLGPTPQASIQWTSGLGKHYVWTAVTGNTFDVFCRQVNHQ
                     VEICKKWGIRVGLHNHLGSLVETQEQLEEFLRRCPECGLILDTAHLAAAGGDPVEIVK
                     KYGNRLVSVHLKDWFVLNPEIGLEQWTKRGRFCELGAGNIGLDNIKVMKEVVKCGYDG
                     WIFIEQDTHLQDPLIDLAKSRNYLKDAGF"
BASE COUNT          879 a          675 c          438 g          902 t
ORIGIN      
        1 acatcttcaa ggagaaagca tacacatgat gggcacacac tggtacaact gccgcactgg
       61 acacagtgtt cccagaccat tccccatttt tttgaaccga acatggattt aaattgttct
      121 gaaatttttt ccggtatatg aaattgttcg ttatacttca caatcttttt tgaaacacct
      181 tctcttaatt tctttctttg ttcaaggaaa gattctgaga catctttcag gagagattta
      241 tcctgcaata atacttcacc cttttcagag ccaacatcaa caacaatacc cccttcaagt
      301 ttcgtcagat tcaggtcaaa tccatcttca ggatatggat ttaatccaag ggatttacaa
      361 aaacaaacag ataggggctg agaacaatct attgaaacaa taaacgtatt ctgtcttctt
      421 tgaatgaaat ttgggtcttt ataatcccct tcaagaaaaa ccctgtccag aattttcaac
      481 ccctgcaaat cgcaattggc agcgccaata ataattcttt ttctggaagt ggcaatttca
      541 ggaacaaccc tttcctgaaa gggataaagg aaaaatttca gtggttgaat tggtcttact
      601 acgcctataa ccgcctcatc gaaattttcc tttgacactg actcatacaa taaatgctgg
      661 cttctggaaa acggtgcaaa aacatcgtaa tcgccaagcc atccggtaaa aagctcttct
      721 attctattat tctcaataaa atatgttttc ataaaacttt gtgaaattaa tcactaactt
      781 tgtcaaaaaa ttaaaagtta tataaattta tcacataaat ttcttttgtc aaatcgttca
      841 aggatagcga taactttaac ctgacataac cttcttacac attaaatact ttatgaataa
      901 aagtagtttc cactactgtg aaataataaa tagcaattat taattcacca tatcaactga
      961 atttcgttcc cttcttcgca gcagaaataa ttctcataac ttcaagcgcc tgcttccatt
     1021 ttacagggaa aggttttttg tttcttattg cctcataaag ataatcccag ataaaatgca
     1081 tctcaacttt taattctgga ttaacaggaa tcgtcttttc aatccagaca agtttctcag
     1141 gtgaaataaa acctacccca ggagttccag gattagcttt taactctcgt aaaggcactg
     1201 aaggatcaag ataacgtaat ttaatctcat ttcctgtgca ttcaagagcg cccttacttc
     1261 ctaaaacaag atattctgac tctgatattg ccgcccctcc actgatttcc aggtcaacga
     1321 ccatttgatt tttaccctta agaatgatgt gaatatggtc ctctgcatca cccgcagcag
     1381 caatcttttt caaatcgctc cacatttctt tcaccggtga gtcaagaaaa cgcaacgcat
     1441 ggtcaacaat gtgaggaccc cagttcagca atagtcctcc accatatttt tttaaggttt
     1501 gccagtcatt cctgcgttga taattattcc gacggagttt tatttcaaaa acctgcccaa
     1561 gaatccctga agcaataatt tctctgatat gctggaacgc tggatcaaaa cgacggttat
     1621 ggcggataaa tagtttcttc ttttggctgg caggcaatct atcaatcttt agcgcctcgc
     1681 tgtaacttgt agccagcggt ttttcaagaa atacatattt atttgccttc agagccatga
     1741 ttgcatgttc cacatggtct ttagaacggc ttgcgatatc aaccagttca acatccggat
     1801 cattgatcag gtcctcaact ctcttgtagg ccttgcatcc atacttttcc acaaaacgtt
     1861 ttctgaaatc ttgtacaata tcacaacctg caacgatttg aaatttatcc ttaaagttat
     1921 caagttcaca acagtgcata ccccatcctg ccctgcccag tcctattatt ccaatcttaa
     1981 ctggatgatt tttcatttca gtttcctcca ttgaatcaaa accctgcgtc tttaagataa
     2041 tttctgcttt ttgcaaggtc tatcagtgga tcctgaagat gcgtatcctg ttcaataaaa
     2101 atccatccat catatccgca tttgacaact tccttcatga ccttaatgtt atcaagtcca
     2161 atattacctg ctccaagttc gcaaaacctt cctcgttttg tccattgttc cagtccaatc
     2221 tcaggattta gcacaaacca gtcctttaag tgtacagaga caagacgatt tccatacttt
     2281 ttcactatct caacaggatc tcctcctgca gccgcaaggt gtgcagtgtc aagaatcaag
     2341 ccacattcag gacaccttct taaaaactct tccagttgtt cctgggtttc tacaagagac
     2401 ccaaggtgat tatggagacc aacacgaata ccccattttt tgcaaatctc aacctgatga
     2461 ttcacctgcc tgcaaaaaac atcaaatgta ttaccagtaa ctgctgtcca cacataatgc
     2521 ttaccgagtc ctgaagtcca ctggattgat gcttgtggag tgggtccgag acatgtggca
     2581 aaatccatac ccagtttcct gtaagttcct gaacgcaaaa gtgcctctga aggactgctg
     2641 gcaacaagtc gttcagtacc gtcataacca atttttttca aatcagtcag acgctgttca
     2701 atatcaaata agccaccata ccatacattc atcccataat cagcaacccc aaatttcatt
     2761 tttctctcct cccctgctaa atttatataa taaatcaact ttgctattaa cagttagatc
     2821 ccatttcaag acaccattta tgatacacta tgttgataaa atttcaaacg attggaaaaa
     2881 ttgaactgtc tgat
//
LOCUS       DAORYU010000186         2865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_23222_length_2865_cov_8.496797, whole genome shotgun sequence.
ACCESSION   DAORYU010000186 DAORYU010000000
VERSION     DAORYU010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23222_length_2865_cov_8.496797"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1438)
                     /locus_tag="PLW07_05965"
     CDS             complement(<1..1438)
                     /locus_tag="PLW07_05965"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HOL50107.1"
                     /translation="MNRREQWSSKIGVILAVAGSAIGLGNFLRFPVQAAKNGGGAFII
                     PYLVSFLLLGIPLMWIEWTLGRFGGGFGHGTAPGMFHNIANKNRFIKYFGVIGIFGPI
                     AIFTYYTYIESWLFGYSIFAITGKLSLCQDQSSMVAFLKGYQGLEQNQFFSSVVPAYI
                     FFLITFAVNIATVAGGIQKGIERVCKYGLPVLFMLGIILMIRVLTLGSMYPEHPDWTP
                     VRGLGFLWNPDFSSLLDAKVWLAAAGQIFFTLSVGIGVILTYASYLKKNDDVILSGLT
                     AAATNEWAEIVLGGSIAITSAFLFFGPEGIKTVAESGAFNLGFVAMPLALNHIPFGNI
                     FGFFWFALLFIAGVTSSISLAQPAIAFLEDEFNIDKKKAVIIFAIITFILCNMCVFLL
                     GKGFLDEFDFWGGTFALVLFGTIEAILFSWVFGIDRAWDEMHAGSNMKLPRFYRFVIK
                     YITPVFLLVILISWFIREAKDVILMKNVP"
     gene            complement(1445..2542)
                     /locus_tag="PLW07_05970"
     CDS             complement(1445..2542)
                     /locus_tag="PLW07_05970"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOL50108.1"
                     /translation="MENVAIVQCSDYKKQTIQGAIKRIFDLLECNFSSGILLKPNMLS
                     SLSPEQAVTTHPVILEVLSEILPHPVYIGDSPPNSQKTIELYWEKCGYASIASNTGAT
                     LVKLEGKSTIFQLNIAGKTVSFPVSDFGLNHPIFNLPKFKTHNITILTLCMKNLYGLI
                     PGYPKGLLHTEFPQPESFNSFIVEFYRMISEKVIFNLVDAVEIMDGNGPASGRKRYYG
                     YLIAGKNAVCVDFVCATLAGLKIHEIPFLRIYETKYGIPGTKIVGDEPEIIDNFQLPL
                     SSVLFRIQRVSALSKFVNTVANHLNVIPVINHRKCVRCMECYNVCPANAISKDLKIDR
                     KKCINCLCCFEVCQHKAIEVKKSFLARIILP"
     gene            complement(2555..>2865)
                     /locus_tag="PLW07_05975"
     CDS             complement(2555..>2865)
                     /locus_tag="PLW07_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50109.1"
                     /translation="VKQGVLESAVYPAEPASYRISWESATPPGTSVKFQIRTATNRES
                     LNDVEFIGPDGTSQSFFEKSGEIFKVAHKGFIQYRVVLTTDDPVKTPYLRRVTISKVE
                     "
BASE COUNT          946 a          612 c          500 g          807 t
ORIGIN      
        1 aaggaacatt tttcatcaaa atcacatctt ttgcttccct gataaaccag gaaatcaata
       61 taacaaggag aaagaccggc gtaatatatt tgataacgaa tctataaaat ctcggcagtt
      121 tcatattgct tccagcgtgc atttcgtccc aagccctgtc aattccaaag acccatgaaa
      181 acaatatcgc ttcaatggtc ccaaaaagaa ccagcgcaaa agtaccaccc cagaagtcaa
      241 actcgtcaag gaatcctttg ccaagaagaa acacacacat gttgcagaga ataaatgtaa
      301 tgatggcaaa tattatcact gcctttttct tatcaatgtt aaattcgtct tcaagaaatg
      361 ctattgctgg ctgggcaaga gatattgaag acgtcacgcc tgcaataaaa agaagcgcaa
      421 accaaaaaaa accaaaaata tttccaaatg gaatatgatt taaagccagt ggcattgcaa
      481 caaaacccaa attaaatgct ccactttcag cgacagtttt tatgccctca ggtccaaaaa
      541 acaaaaatgc tgaggtaatg gcaatactgc caccaagcac aatttctgcc cattcattgg
      601 ttgctgctgc agtcagtcca gaaagtatca catcatcatt tttcttaaga taactggcat
      661 aggtaagaat cactccgatt ccgacactca acgtaaagaa aatctggcct gctgctgcaa
      721 gccatacctt tgcatcaagg agactggaaa aatcaggatt ccacaggaaa ccaagtcccc
      781 ttacaggtgt ccagtcagga tgctctggat acattgaacc aagagtaaga accctgatca
      841 tcaaaataat tccaagcata aaaagtacag gtaatccgta tttacaaacc cgttctattc
      901 ctttttgtat tccacctgct actgtggcaa tattaactgc aaatgtaatg agaaaaaaga
      961 tgtacgcagg aacaactgaa gaaaaaaatt gattttgttc aagcccctga taacctttca
     1021 aaaacgcaac cattgacgat tggtcctgac acaaagaaag tttgcctgta atagcaaaaa
     1081 tactgtatcc aaaaagccag gattcaatat atgtgtaata ggtaaaaatt gcaattggtc
     1141 caaaaattcc tatcacgcca aaatacttga taaacctgtt tttgtttgcg atgttgtgaa
     1201 acattcctgg agcagtacca tgaccgaagc caccgccaaa tcttccaagc gtccattcaa
     1261 tccacatcaa agggattcca agaaggagaa aagaaacaag atatggaata atgaacgcac
     1321 caccgccatt ttttgctgcc tggactggaa aacgtaaaaa gttaccgaga ccaatggcgc
     1381 tgcctgcaac cgcaaggatc accccaattt ttgaactcca ctgttctctt ctgttcatat
     1441 ccggctatgg taagataatc cgtgcaagaa aacttttttt tacctctatc gctttatgct
     1501 ggcagacctc aaaacagcac aaacaattta tacacttttt tctatcaatt ttcaagtcct
     1561 ttgatatggc gtttgcagga caaacattat agcattccat gcacctgacg cattttctgt
     1621 gattgataac tggtatgaca ttaagatggt ttgccactgt gtttacaaat tttgacagtg
     1681 ctgaaacccg ctgaattcta aaaagcactg atgacaaagg caattgaaaa ttatcaataa
     1741 tttccggttc atcacccaca attttagtcc ctggtatgcc atatttcgtt tcataaatac
     1801 gcaaaaatgg aatctcatgt attttcaaac ctgccaaagt tgcgcaaaca aaatccacac
     1861 acacagcgtt tttccctgca atgagataac cataatacct ttttcttcct gacgctggac
     1921 catttccatc cataatttca acagcatcca caagattaaa aattactttt tctgatatca
     1981 ttctgtaaaa ttcaacaata aaactgttaa aactttctgg ttgcggaaac tcagtatgaa
     2041 gaagtccctt gggatagcca ggaatgagtc cataaagatt tttcatacaa agtgtaagaa
     2101 ttgtaatatt atgggttttg aattttggaa gattgaaaat tggatgattc agtccaaaat
     2161 cagaaacagg aaaagaaaca gttttccctg caatattcaa ttgaaaaatg gtacttttgc
     2221 cttccagttt cacaagtgtt gcgcctgtat ttgaagcaat agaagcatac ccacattttt
     2281 cccagtacaa ctcaattgtt ttctggctgt ttggtggact atctccaata taaacagggt
     2341 gtggaagtat ttcagacaac acctcaagaa taacaggatg ggtggtaact gcctgttctg
     2401 gactgagaga tgaaagcatg tttggtttca gaagtatgcc agatgaaaaa ttgcattcaa
     2461 gcaaatcaaa aatccttttt atcgctcctt gaattgtttg cttcttataa tcagagcatt
     2521 gtacaatcgc aacattttcc attgataggt tttttcattc cacctttgaa attgttacgc
     2581 gcctcagata cggtgttttt accgggtcgt ctgttgtcag tactaccctg tattgaataa
     2641 atcccttgtg ggcaacttta aaaatctcgc cggacttttc aaaaaaactt tgagaggtcc
     2701 catcaggacc gataaattcg acatcattca aagattctct gtttgtggcg gtgcgaattt
     2761 gaaattttac tgatgtccct ggaggtgttg ctgattccca ggaaattcta taagacgctg
     2821 gctcagcagg atatactgcg gattccagaa caccctgctt cacaa
//
LOCUS       DAORYU010000187         2857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_23307_length_2857_cov_4.940757, whole genome shotgun sequence.
ACCESSION   DAORYU010000187 DAORYU010000000
VERSION     DAORYU010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23307_length_2857_cov_4.940757"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="PLW07_05980"
     CDS             complement(<1..766)
                     /locus_tag="PLW07_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOL50110.1"
                     /translation="MKVYNFSSSVEEIAIKQSGFEPQYLPEEKIRILEVPDFPSLGKI
                     TALRFIEWCQKNPGGVISLPTGKTPEYFIQWTSFILNNWNSKGVKELLEKYAIDVLSK
                     PDMFSMVFVQIDEFFPMNPEQENSFVWYIKKFYVKKFGLDENKTILINAWTTGCKQGE
                     NLGELFPNGKIDLSLRYRQPSNRFEELQQKAIFSADQYAMEYEQKIRELGGIGFFLGG
                     IGPDGHIGFNIKGSDHFSTTRLAFINYETAAAAATDL"
     gene            complement(785..1411)
                     /locus_tag="PLW07_05985"
     CDS             complement(785..1411)
                     /locus_tag="PLW07_05985"
                     /inference="COORDINATES: protein motif:HMM:NF016051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HOL50111.1"
                     /translation="MNILVTAGPTREYIDPVRFISNPATGTLGYLIAKKAKEKAHSVV
                     LVAGPCEFPEIKGVKKIQVESALEMKHEVEKIFPEMDALIMTAAVSDWRPVKKSGEKL
                     KLKKSWTLKLVPNPDILKCLSKKKKKNQIIVGFALESTNLVENARKKLMDKKLDMIVA
                     NTIKNFGKFTGSGEIFMLYSDGRIKNCTGFSKQVLASFLLKEIEKLQR"
     gene            complement(1408..2520)
                     /gene="prfB"
                     /locus_tag="PLW07_05990"
     CDS             complement(1408..2520)
                     /gene="prfB"
                     /locus_tag="PLW07_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004102881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOL50112.1"
                     /translation="MMEQTLKNKSDELLAIFDDIKKRIRWEQQEQRFTSLENEKNTKG
                     YWDPKLDGEKMKEWNQLAEKRMKVDKITRLFENFSASVELLEENAAPELEREASGILE
                     EITKEIEDFKTELYFSDPADRNNAILTLHAGAGGTEACDWVSMLFRMYSKWAAKKNIK
                     VEVVDFLPGEEAGLKKITAVFSGDYAYGYLKGETGVHRLVRISPFDANKRRHTSFAAV
                     NVIPEVDRDIKIEIKEEDIEFETFRSGGKGGQNVNKVETAVRIKHIPTGIIVQCQNER
                     SQFKNREMAMKILRSRLYQIQMEKERKKEAEQWDAQKDIAWGSQIRSYVFCPYTMVKD
                     HRTQVETGNVDAVMDGEIDQFIQAEIEYLALEKNEK"
     gene            complement(2520..>2857)
                     /gene="obgE"
                     /locus_tag="PLW07_05995"
                     /gene_synonym="cgtA"
                     /gene_synonym="obg"
                     /gene_synonym="yhbZ"
     CDS             complement(2520..>2857)
                     /gene="obgE"
                     /locus_tag="PLW07_05995"
                     /gene_synonym="cgtA"
                     /gene_synonym="obg"
                     /gene_synonym="yhbZ"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="ObgE; essential GTPase; exhibits high exchange rate
                     for GTP/GDP; associates with 50S ribosomal subunit;
                     involved in regulation of chromosomal replication; Derived
                     by automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOL50113.1"
                     /translation="LIEGASLGAGLGIKFLRHIERTKVLVHLIDLSLPEPAKRYYNIR
                     NEIGCYDATLLEKPEIIVGTKIDLSESRNNIQKLGKIADRYFLISCVTDEGIKPLLEE
                     IWRILTEGY"
BASE COUNT          760 a          658 c          430 g         1009 t
ORIGIN      
        1 ccaagtcagt cgctgctgct gccgcagttt cataattaat aaaggcaagt cgtgttgtac
       61 tgaaatggtc tgaccctttg atattaaaac caatgtgtcc atcaggacct attccaccaa
      121 gaaaaaatcc aataccgcca agttctctta tcttttgctc atactccatt gcatactgat
      181 cggccgaaaa aattgctttc tgttgaagtt cttcgaaccg gtttgatggc tgcctgtatc
      241 ttaacgataa atctattttt ccattcggga aaagttcacc aagattttct ccctgtttgc
      301 aaccggttgt ccacgcatta atcaaaattg ttttattttc gtccagccca aattttttca
      361 cataaaattt ttttatgtac cacacaaaac tattttcctg ttctggattc atgggaaaaa
      421 attcgtctat ctgcacaaaa accattgaaa acatatcagg ttttgacagg acatcaattg
      481 catatttttc aagaagttct tttaccccct tactgttcca gttattcaga ataaaggacg
      541 tccattggat aaaatattcg ggggtcttgc ctgttggcaa tgatatcacc ccacctggat
      601 ttttctgaca ccattcaata aatctcaatg ctgttatctt accaagactt ggaaaatcag
      661 gaacctcaag aatcctgatt ttttcctcag gtaagtattg aggttcaaac ccagattgtt
      721 ttattgcaat ctcttcaact gatgaagaaa aattataaac tttcattttt tcccctttca
      781 aatttcatct ctgaagtttt tcaatttctt tcagtaaaaa agaggcaaga acctgcttgg
      841 agaaacccgt acaattcttg attctgccat cagaatataa cataaaaatt tcgccactac
      901 cagtaaattt tccaaaattt tttattgtgt ttgcaacaat catatcaagt ttcttatcca
      961 ttaatttctt tctcgcattt tccaccaggt tggtactttc cagagcaaaa ccaacaataa
     1021 tttgattttt tttctttttc tttgaaagac atttcagaat atccggattc ggcacaagtt
     1081 ttaatgtcca ggatttttta agtttcaatt tttcaccaga tttctttaca ggtctccagt
     1141 cagaaactgc cgcagtcata atcagcgcgt ccatctctgg aaatattttt tctacttcgt
     1201 gtttcatttc cagcgccgat tctacctgaa tttttttcac ccctttaatt tcaggaaatt
     1261 cacacggacc tgcaacaagc accactgaat gcgccttctc ttttgctttt tttgcaataa
     1321 gatagccaag agttcctgta gcaggattgc ttataaatct gacagggtcg atatattccc
     1381 ttgttggtcc cgctgtgaca agtatgttca tttttcgttt ttctcaagcg caagatattc
     1441 aatctctgcc tgaatgaact ggtctatttc tccatccatt actgcgtcaa cattacctgt
     1501 ttcaacctgc gtccgatggt ctttcaccat tgtatatgga cagaacacat aggatctaat
     1561 ctgacttccc cacgcaatgt ctttttgagc gtcccattgt tctgcttctt ttttcctttc
     1621 tttttccatc tgtatttgat acaggcggct gcgcaaaatt ttcatcgcca tttcgcgatt
     1681 tttaaactgg cttctttcat tctgacattg cacaataatt cccgttggta tgtgttttat
     1741 tcttactgcg gtttcaacct tattgacatt ctgcccgcct ttaccaccag aacgaaatgt
     1801 ctcaaattca atatcttctt cttttatttc tatcttgatg tccctgtcca cttctggaat
     1861 cacgttaaca gcagcaaaac tggtatgacg acgtttatta gcatcaaaag gagatatcct
     1921 cacgagtcga tgaacccctg tttcaccttt taaatatcca tacgcataat cgccagaaaa
     1981 aaccgctgta atcttcttca agcccgcttc ttctcctggc agaaaatcaa ccacttcaac
     2041 ttttatattt tttttcgctg cccactttga atacattcta aaaagcatac tcacccaatc
     2101 acatgcttct gtaccgccag cgcccgcatg aagcgtaaga atcgcattat ttctgtctgc
     2161 aggatcagaa aaataaagtt ccgtcttaaa atcctctatc tcctttgtga tttcttcaag
     2221 aatcccggat gcctcccttt caagttcagg ggcagcattt tcttcaagaa gttcaacgct
     2281 cgcagaaaaa ttttcaaata atcttgttat tttatcaact ttcatgcgtt tctcagcaag
     2341 ttgattccat tccttcattt tttcaccgtc aagttttgga tcccagtatc ccttcgtgtt
     2401 tttttcgttc tcaagggaag taaatctctg ttcctgttgt tcccatctaa tccttttttt
     2461 gatatcatca aaaatggcaa gcagctcgtc gcttttattt tttaatgttt gttccatcat
     2521 caatatccct ctgtaagaat cctccagatt tcctcgagta atggcttgat accttcatct
     2581 gtcacgcagg aaataagaaa gtatctatct gcaatttttc ccagtttttg aatattgttt
     2641 cgactttctg ataaatctat ttttgtccca actataattt ctggtttttc cagcagcgtt
     2701 gcatcataac agccaatctc gtttctgata ttataatatc tttttgcagg ttctggtaga
     2761 gaaaggtcaa taagatgaac cagaactttt gtacgttcaa tatggcgtag gaatttaata
     2821 ccgagtcctg cccccagaga ggccccttca ataagcc
//
LOCUS       DAORYU010000188         2814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_23796_length_2814_cov_3.245016, whole genome shotgun sequence.
ACCESSION   DAORYU010000188 DAORYU010000000
VERSION     DAORYU010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23796_length_2814_cov_3.245016"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PLW07_06000"
     CDS             complement(<1..223)
                     /locus_tag="PLW07_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOL50114.1"
                     /translation="MKGRGFTLIELLVVIAIIAILAALLLPVLSKAREKARQSVCIGN
                     LKQIGLAFLMYVQDNNEFMPATFYSVSPVG"
     gene            complement(282..1043)
                     /locus_tag="PLW07_06005"
     CDS             complement(282..1043)
                     /locus_tag="PLW07_06005"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOL50115.1"
                     /translation="MRKNKNCYDGIPLQTGRLKKTKGFTLIELLVVVAIIAILAATLL
                     PVVQRAMENARRAACLSNVKQMGFAMNLYLLDNDDYFPMVVWNKSIPFFEYWAHWMKQ
                     IAPYIDPKIDLTTKTINDSNLATSIDRFIQVYQCPTTWQSRAISWLGHSYGYNFTLQF
                     GVSWWGTPFIKKAGKIPEPARFYVLSDASSYIVQLPRTFATFTTGSPYTQWGARHHFY
                     GINILFADGHAEYVPPPNPNPTDRDEPWSTKLRMW"
     gene            complement(1054..1770)
                     /locus_tag="PLW07_06010"
     CDS             complement(1054..1770)
                     /locus_tag="PLW07_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOL50116.1"
                     /translation="MKKALTKKHQVSKICGFTLIELLVVIAIISILAAMLLPVLSKAR
                     ENTRRSVCASNMKQLYTIFTIYAQDFDGYYPHWTSQNILRALGILQTEGYVKSPEIFI
                     CPSAKRRDHMGRNTRISQTCPGTCTGVQYHCSYACIFGGHNMMFATDPAYKNWALITD
                     ETDKWYYTIQIQSSIWLAKQDTTASPYQPRITGANLNHGWNGLNVLFNDGRVEWISGP
                     NLQSNIQNPDQLMNPFTFVK"
     gene            complement(2006..>2814)
                     /locus_tag="PLW07_06015"
     CDS             complement(2006..>2814)
                     /locus_tag="PLW07_06015"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOL50117.1"
                     /translation="FSPFFVELTGRIHQLLRKKNYVCVSIDVGAEREFDEAMEIFLSR
                     RVDGLICLISFLGAEDILRIKNENLRVVFYHENFEYPADIVAVNAYKAGSMTVEHLIG
                     LGHKKIGYIGKGIDESTDRRYAGFKDTIIRNNLHFNNKWIITGYGSFQTGFHGMRKLL
                     SLKDRPTAIIAHNDVVAIGAVSAVYQAGLKVPQDIAIVGFDNIENSRYLNVPLTTVEF
                     PIEKIAKELVEILFQKIEYNKDNEKYRKFIFEPRLIIRESCGYHLVKGSA"
BASE COUNT          808 a          583 c          484 g          939 t
ORIGIN      
        1 gaccaacagg tgaaactgaa taaaatgtgg ctggcataaa ttcgttgtta tcctgtacat
       61 acattagaaa ggccagtcca atttgtttca aattgcctat gcatactgat tgtcgtgcct
      121 tttctcttgc tttacttagc acaggtagaa gcagggctgc aagaatcgcg ataatcgcga
      181 ttaccacaag gagctcgatg agtgtaaatc ccctgccttt caaagataat ctctttgtat
      241 tgattctgca tcacctcctt ttcttctatt ttttttcaaa tttaccacat tctcaacttt
      301 gtactccagg gttcatccct atcagttgga ttgggatttg gtggcggaac atattctgca
      361 tgcccatctg caaaaaggat attaattccg taaaaatgat gccttgcccc ccactgagta
      421 taaggagaac cagtagtaaa tgtagcaaaa gttcttggca gttgtacaat ataactactg
      481 gcatcactca gaacataaaa tcttgctggt tcaggaattt ttcctgcctt ttttataaaa
      541 ggcgtgcccc accaacttac accaaattgt aaagtaaagt tgtatccgta actatgtcca
      601 agccatgaaa ttgccctgct ttgccatgtg gttggacact ggtaaacctg aataaatcgg
      661 tctatggaag ttgctaaatt tgaatcattg atggtttttg ttgtcaggtc aatttttgga
      721 tcaatgtatg gagcaatttg tttcatccag tgagcccagt attcaaaaaa aggaatactc
      781 ttattccaca caaccatggg gaaataatca tcattgtcca gtaaatacaa attcatagcg
      841 aaccccatct gtttgacatt actcaggcaa gctgctcttc ttgcgttttc catcgctctt
      901 tgaactactg gaagcaatgt tgctgcaaga atagcaataa ttgctacaac aaccagcaat
      961 tctatcaagg tgaacccctt tgtttttttc aaccttcctg tctgtaatgg tattccatca
     1021 taacaatttt tattttttct catagcatcc cccttactta acaaatgtaa atggattcat
     1081 aagttgatcc gggttttgta tattgctttg aagattgggt cctgatatcc actcaactct
     1141 tccatcgtta aacaaaacat tcagtccatt ccaaccatgg ttcaggtttg cgccagtaat
     1201 ccttggttga tatggactgg ctgttgtgtc ctgttttgcc agccagattg aactttgtat
     1261 ctgaattgtg taataccatt tgtctgtttc atcagtaatc agagcccagt ttttgtatgc
     1321 agggtcagtg gcaaacatca tattatggcc tccaaaaata caagcatacg aacaatgata
     1381 ttggacccca gtacatgtgc caggacacgt ctgggaaata cgggtgtttc ttcccatatg
     1441 gtctcttctc ttggctgaag gacatatgaa aatttcagga ctttttacat atccctcggt
     1501 ctgtagtatc ccgagagcgc gaaggatatt ctgcgatgtc cagtgaggat agtatccatc
     1561 aaaatcttga gcatatattg taaaaatcgt ataaagttgc ttcatattac ttgcacaaac
     1621 acttcttctt gtattttctc ttgctttact cagtacagga agcaacattg cagcaaggat
     1681 tgaaataatc gcaattacaa ccagtagctc tatcaaggta aaaccgcata tcttacttac
     1741 ctgatgcttt tttgtaaggg cctttttcat aaatgcctcc atttaaaata tttcattttc
     1801 acttttatgt aatcatctgg tttttgccag atgtgaaaca gggaatctat atcatcatcc
     1861 accatgatag atatggcaga tggaaattga aaaatggcca ttgtatctgt aaaaagttgt
     1921 gatatgtgat caaaattggt ttgtacaaga ggaatgaatc ctgtggattt aagcgctttt
     1981 tttgaatatt ctgttttcaa cctcattatg ctgatccttt cactaaatga taaccgcacg
     2041 attctctgat aattaaacgt ggctcaaaga taaactttct gtatttttca ttatctttgt
     2101 tatattcaat tttctggaaa agtatttcta ccagttcctt tgcgattttt tctatcggaa
     2161 actcaactgt tgtcagaggc acattgagat accttgaatt ttcgatatta tcaaaaccaa
     2221 caatggcaat atcctgtggg actttaagac ctgcctgata aacagcactc actgcaccaa
     2281 tcgcaacaac atcgttatga gcaataattg ctgtgggtct gtctttcaaa ctcagcaatt
     2341 tcctcattcc atgaaaacct gtttgaaaac taccatatcc agtgatgatc catttattgt
     2401 taaagtgtaa attgtttctt atgatggtat ccttaaatcc tgcatacctt ctgtctgtgg
     2461 attcatctat tcctttgccg atataaccaa tttttttgtg ccccagtcca attaagtgtt
     2521 ccacagtcat tgaaccggct ttgtatgcat tcactgctac aatgtcagca ggatattcaa
     2581 aattttcgtg ataaaaaact accctcagat tttcattctt gatacggagt atgtcttcgg
     2641 ccccgaggaa tgaaattaaa catattagtc catccactct ccttgaaagg aatatttcca
     2701 tcgcttcatc aaattctctt tcagccccaa catctataga tacgcagacg taatttttct
     2761 ttcttaacaa ttgatgaatt cttccggtta actccacaaa aaatggactg aaag
//
LOCUS       DAORYU010000189         2809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_23849_length_2809_cov_4.513435, whole genome shotgun sequence.
ACCESSION   DAORYU010000189 DAORYU010000000
VERSION     DAORYU010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23849_length_2809_cov_4.513435"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            398..1279
                     /locus_tag="PLW07_06020"
     CDS             398..1279
                     /locus_tag="PLW07_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOL50118.1"
                     /translation="MTLKSEEFNKIIEKRSLSAEVKWMKIFHPFIARKAQPGQFIIVR
                     PTEESERIPLTLASASPEIGTIEIIFQEVGTTTKKLGTLEAGTKINNVAGPLGKPIHI
                     EKFGRVLFVTGGVGSAFVYWMAKAFKEKGNSVWTVMGARNANLLILENEMRNICDKVL
                     IATDDGSKGDKGFVTDVLLNALKSGEHADLAICAGPVMMMKNVAKITKEHNIKTIASL
                     NPIMIDGTGMCGGCRVTVGGKVMFACVDGPDFDAHQVDFDELQKRTGFYKKHEQCSLE
                     KYMNNATKDDFISTSGQ"
     gene            complement(1288..>2809)
                     /locus_tag="PLW07_06025"
     CDS             complement(1288..>2809)
                     /locus_tag="PLW07_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOL50119.1"
                     /translation="ERYEVHHGVKINDNAIVAAAILSHRYISDRFLPDKAIDLIDEAA
                     SRLRMEMESKPQELDEIDRKIMQLEIEREALKKETSESSKERLKKIEHELEQLKKEKA
                     ELDTQWEHEKKKIEKIKEIKQQVELAKKIIEDAEKKGDLGTAAEYKYGKLIELEKQLQ
                     AETQNLRRDQSSRKLIKDEVDEEEIATIVSRWTGIPVNRMLEGEIEKLIHLEERLSKR
                     VVGQKEAIIAVSDAVRRNRAGLSDPKRPIGSFIFLGPTGVGKTELAKALAEFLFNDEN
                     ALVRIDMSEYMEKFSVSRLIGAPPGYVGYEEGGQLTERVRRRPYTVVLLDEIEKAHSE
                     VFNILLQLLDEGRLTDGQGRTVDFRNTVIIMTSNLGSQYFEMFKDQEEIEKKVLELLK
                     SKFRPEFLNRVDEIILFHKLSKSDLLKIVDIQISYLASRLADKKITIQLTTAARKWLA
                     EHGYDANYGARPLKRLIQKEIENPLAIKLLSGEFTEGSDIEIDESDNKLIFRRLSK"
BASE COUNT          813 a          564 c          544 g          888 t
ORIGIN      
        1 aaaccaaaaa aataggaaaa tacccaatgg atgacagcaa aatattattc acatatgctg
       61 cggtgcagac aaaactacgg ataaatccgc tgcacctgca aacttgcctt ctccgtatac
      121 gccttgcact gcgaaggctt cgaacaagca ggtgtaaggt tatcgcggaa cgcagatgct
      181 tttatccttg ttttgttgct gcgccttcgc taattgttca taatgcttct gtctttcctg
      241 cgggaagtgg ggaaagaaga aaaccaaaaa aacaggaaaa atacccaatg gatgacagca
      301 aaatattatt cacatacgct gcggtgcaga caaaactacg caggaatctg ccgcagttga
      361 aacttgtcag caccgtgggc gcttaaaagg gagaaaaatg acattaaaaa gcgaagaatt
      421 caacaagatt attgaaaaaa ggtctctttc tgctgaagta aaatggatga aaattttcca
      481 tccattcatt gccagaaagg cgcagccagg tcagtttatc atcgtaagac ccactgaaga
      541 aagtgaaagg attccactta cacttgcaag tgcatctcct gaaataggta ccattgaaat
      601 aattttccag gaagtcggca cgacaacaaa aaaacttggt actctggaag caggcacaaa
      661 aatcaataat gttgcaggtc ctcttggaaa acctattcac attgaaaagt ttggtagagt
      721 tctttttgtc actggaggcg tcggttcagc atttgtgtac tggatggcta aggcatttaa
      781 ggaaaaaggc aattctgtat ggacagtaat gggcgcaaga aacgcaaatt tattgatact
      841 tgaaaatgaa atgcgcaata tttgcgataa ggttctgata gccacagatg atggaagcaa
      901 aggagacaag ggttttgtga ctgatgtttt actgaacgct ctgaaatcag gagaacatgc
      961 agatttggca atatgcgcgg gtcctgttat gatgatgaaa aatgttgcaa aaattacaaa
     1021 ggaacacaat ataaaaacca ttgccagttt aaatcctatt atgattgatg gaacaggaat
     1081 gtgtggtggg tgtcgtgtaa ctgttggtgg aaaggtaatg tttgcgtgtg ttgatggtcc
     1141 ggattttgac gcacatcagg ttgattttga tgaactgcaa aaaagaactg gcttttataa
     1201 aaaacacgaa caatgctcac ttgaaaaata catgaacaat gccacaaagg atgattttat
     1261 ttccacctct ggtcaatgat gctgatatta tttagacagc cgtctgaaaa tcagtttgtt
     1321 gtctgactcg tctatctcaa tgtctgagcc ctcagtaaat tcgccagaaa gtaacttaat
     1381 agcaagagga ttttcaattt ccttctggat aagccgtttg agtggcctgg caccataatt
     1441 agcatcgtaa ccatgttcag ccaaccattt tcttgctgca gtagtaagtt gaatggtaat
     1501 ttttttatca gccagacggg aagcaagata tgaaatctgt atatccacaa ttttcaaaag
     1561 gtctgatttt gaaagtttat ggaaaagtat aatttcatca actctgttaa gaaattcagg
     1621 tctgaattta gattttaata attcaagcac cttcttttca atttcttcct ggtccttgaa
     1681 catttcaaag tattggctgc caagatttga tgtcattata attacggtat ttctaaaatc
     1741 aacggttctg ccttgtccgt ctgttaacct tccctcatca agtaattgaa gaagaatatt
     1801 gaacacctcg ctgtgtgctt tttcaatctc gtccagcaaa actactgtat atggtcttcg
     1861 cctgaccctt tcagtaagtt ggcctccctc ttcatatcca acatatccgg ggggagcccc
     1921 aatcaaacga ctgactgaaa atttttccat atactcagac atatctatgc gaacaagagc
     1981 attttcatca ttaaaaagaa actcagcaag cgccttggca agttcagttt taccaacgcc
     2041 agttggacca agaaaaataa acgaaccaat cggccttttg gggtctgaaa gtccggcacg
     2101 atttcttcta acggcatcgg atacagcaat gattgcttct ttttgtccga ccaccctttt
     2161 cgacagtcgt tcttcaagat gaataagttt ttctatctcg ccttcaagca ttctgttgac
     2221 tggtatacca gtccacctgg aaacaatggt cgctatttct tcttcatcca cctcatcctt
     2281 aatgagcttt ctgctgcttt gatcccttct gagattttgt gtttctgctt gaagttgttt
     2341 ttcaagctct attaatttcc cgtatttata ttctgctgct gttccaaggt cacccttctt
     2401 ttcggcgtct tcaattatct tttttgccag ttcgacttgc tgtttaattt ctttgatttt
     2461 ctcaattttc tttttctcat gttcccactg ggtgtcaagt tctgcttttt ctttttttag
     2521 ttgctcaagt tcatgctcga tttttttcaa acgttccttt gatgattctg atgtttcttt
     2581 ttttaatgct tctctttcta tctccagttg cataattttc ctgtcaatct catcaagttc
     2641 ttgaggtttg ctttccattt ccatacgcaa tcggcttgcg gcctcgtcaa tcaaatcaat
     2701 ggctttgtca ggaaggaatc tatcagaaat atatctatga gacagaatag cagctgctac
     2761 aatagcattg tcattgattt ttactccgtg atgcacttcg tatctttct
//
LOCUS       DAORYU010000190         2803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_23936_length_2803_cov_3.834425, whole genome shotgun sequence.
ACCESSION   DAORYU010000190 DAORYU010000000
VERSION     DAORYU010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23936_length_2803_cov_3.834425"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1205
                     /locus_tag="PLW07_06030"
     CDS             <1..1205
                     /locus_tag="PLW07_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50120.1"
                     /translation="IVTEDYTKKIRKYAESLGFAPESVMVHAVQNHSAPSLGHFMLDP
                     DFPPLPEKFEYVRGGETKTYKFIFERVCHAIKMANETLVPVVPVAGRACFDGLAFNRR
                     AVTKSGKVRMPWFYAKAQQPLGPVDIKYIEGPIDPEIGVLCFQRENMKFHSGILHFTC
                     HPVNVFATDYSAISSDWPGAWIQELEKFFEAGCMVANGCCGNINPWPAFEPDFYPDHK
                     KMGGRLAEISEKIITRLIKSNSGTVAFKSKILEIPLRTEQDITEKAKKFLEENAEPIV
                     MKDNPERVDRQWFEAASIMSVEYARKRGFTYEIQAFRVGDFAILGLPGEPFVEGQLEI
                     KTMSPFDFTFIAHATNQYVGYIPIPQALKRGGHEAKFCYWSKLVPEALEIIVQHSISL
                     LNELYQEG"
     gene            1273..2721
                     /locus_tag="PLW07_06035"
     CDS             1273..2721
                     /locus_tag="PLW07_06035"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HOL50121.1"
                     /translation="MTNTFFSDGAVLQRDTKIPVWGTAEPGENIGVKIDNISISTTAD
                     QSGNWMVILPEHKAGGPYEMIVTGKNNITIKDIYFGDVWFCSGQSNMAMTVAGCLNAE
                     TEIKQATNSLIREFKVSSSFSANQMKNLRGSWKVCSSTNVGRFSATAYFFAKNLQPEI
                     GVAVGIINSSVGGTRIEAWMSRQALDKIPDLKQTLDEYDRNTKEYQVALEAYNRKLKE
                     WEELVEEAKTKGIQVPEKPQPPKSVLNANSYSALFNGMVFPVIPFPVKGILWYQGEAN
                     AGKSAYLYRDLLGALIDDWRNAWKQELPFIFVQLPNFGKVVDTMENSSWAVLRESQLM
                     VLKKPKTAMAVAIDIGDAGNIHPKNKQEVGRRLALAALGTVYGKKIVYSCPIYAGMSA
                     EGNKIRIKFNHTGSGLVAKDSDTGEVKGFVIAGEDKKFVKAKAQIEHDTVVVWSDEIT
                     KPVVVRYAWAGNPVCNLYNKEGLPASPFRTDQ"
BASE COUNT          901 a          462 c          658 g          782 t
ORIGIN      
        1 caatcgtaac agaagattac acaaaaaaaa tcaggaaata tgcagaatct ctcgggtttg
       61 cgcctgaatc tgtgatggtg catgctgtac agaatcattc tgcaccgtct ctcggtcatt
      121 tcatgcttga tcctgatttc ccaccactgc cagaaaaatt tgaatatgta agaggtggtg
      181 aaacaaaaac ctacaagttt atttttgaaa gggtatgtca tgcgataaag atggctaatg
      241 aaacccttgt gcctgttgta cctgttgctg gtagagcatg ttttgatggg cttgctttta
      301 acaggcgggc tgtgacaaag agcgggaaag tgagaatgcc atggttttat gcgaaggcgc
      361 aacagccact tggtcctgtg gatatcaaat atattgaagg accaattgac cctgaaattg
      421 gagtactgtg ttttcagaga gagaatatga aatttcatag cgggatactt cattttacct
      481 gccatcctgt gaatgttttt gcgacagatt actctgcgat ttcttctgac tggccaggag
      541 catggattca ggaacttgaa aaattttttg aagcagggtg catggttgcc aatggttgtt
      601 gtggaaatat aaatccctgg ccagcatttg aaccagattt ctatccagac cataaaaaaa
      661 tgggtggacg gcttgcagaa atttcagaaa aaattattac ccgtctgatt aaatcaaatt
      721 caggcacagt tgcgtttaag tcaaagatac ttgaaatccc tttgcgaacc gaacaagaca
      781 ttacagaaaa agcgaaaaaa ttccttgaag aaaatgccga acctattgtt atgaaggata
      841 atcctgaaag agttgaccgc cagtggtttg aagcagcatc aattatgagt gttgaatatg
      901 cgcgaaaaag aggatttacc tatgagatac aggcatttcg ggttggtgat ttcgcaatac
      961 ttggactacc aggagaacca tttgtggaag gtcaactgga aataaaaaca atgtctcctt
     1021 ttgattttac atttatcgcg cacgcaacaa atcaatatgt tggttatatc ccaattccac
     1081 aggcgctgaa aaggggagga catgaagcaa aattttgcta ctggtcaaaa cttgtgccag
     1141 aagcacttga aatcatcgtc caacattcaa tatcgttatt gaatgaactt tatcaggaag
     1201 gttgatatgc tgaagaaaat tttaatgttc ttgtttttaa tggttttagt aggtatctgt
     1261 agggcagatg tagtgactaa tactttcttt agtgatggtg cagtgttaca gagagataca
     1321 aaaattcctg tatggggaac tgcagagcca ggagaaaaca ttggtgtaaa aattgataat
     1381 atttctattt caacaactgc tgaccaatca ggcaactgga tggttatcct accggagcat
     1441 aaagcaggcg gtccctatga aatgattgtg accggaaaaa ataacattac gatcaaagat
     1501 atctattttg gtgatgtgtg gttttgttca ggccaatcca atatggcaat gactgttgca
     1561 ggttgtctga atgcagaaac agaaatcaaa caggcaacaa acagtttaat cagggaattt
     1621 aaagtgagtt cttcctttag tgctaatcaa atgaaaaatt tgcgtggttc ctggaaggtt
     1681 tgcagtagta ccaatgtggg aagattttca gcaaccgcat acttttttgc aaaaaacctt
     1741 caacctgaaa ttggagttgc tgtgggtatc atcaatagtt ctgttggagg tacaagaata
     1801 gaagcatgga tgagcaggca ggcgcttgat aaaattcctg atttgaaaca aacccttgat
     1861 gaatacgaca gaaataccaa ggaataccag gttgctttgg aagcatataa caggaaactg
     1921 aaggaatggg aagaattggt tgaagaagca aaaacaaagg ggattcaggt acctgaaaaa
     1981 ccacaaccac caaagtcagt tctgaatgca aattcataca gtgcgttatt caatggtatg
     2041 gtgtttcctg tgataccatt tccagtaaaa ggaattttgt ggtatcaggg cgaagcgaat
     2101 gcagggaaaa gtgcgtatct ttacagagat ttgctcggtg ctttgattga tgactggaga
     2161 aacgcgtgga aacaggaatt accttttatt tttgtgcaac tgccaaattt cggaaaggtt
     2221 gttgatacaa tggaaaacag ttcctgggca gtactgagag aatcgcaact gatggttttg
     2281 aaaaaaccaa aaacagcgat ggcagtggcc attgatattg gtgacgcagg gaatatccat
     2341 ccaaaaaata agcaagaggt cggaagacga cttgctcttg cagcgcttgg cactgtttat
     2401 ggaaagaaaa ttgtttattc atgtcccatt tacgcaggaa tgagtgctga aggaaataaa
     2461 atcaggataa aatttaatca tacaggcagc gggctggtgg caaaggattc agataccgga
     2521 gaggttaaag gttttgttat tgctggagaa gataaaaaat ttgtgaaggc aaaggcacag
     2581 attgaacatg ataccgtcgt tgtctggagt gatgagatta caaaaccagt ggtagtaaga
     2641 tatgcctggg caggtaatcc tgtgtgcaat ctttacaata aagaaggtct tcctgcatct
     2701 ccttttagaa cagaccaata gtggtggaag aatgttgtaa tagaactttt tcaatgagag
     2761 cgattttcat taatccgcct gtgtacgatt tttctgcatt tga
//
LOCUS       DAORYU010000191         2788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_24099_length_2788_cov_5.271862, whole genome shotgun sequence.
ACCESSION   DAORYU010000191 DAORYU010000000
VERSION     DAORYU010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24099_length_2788_cov_5.271862"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="PLW07_06040"
     CDS             <1..926
                     /locus_tag="PLW07_06040"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOL50122.1"
                     /translation="MNIDTKKIESAITEKTKAIIVVHLHGLAVNMEEVLSIAKKYNLK
                     VIEDCCQAHGAEFKGKKVGLWGDCAAFSFNQNKCLCSGEGGMFVTDNEETYKKALMLW
                     SFGETRAPSEKRDYHAYALGWMYRNNDLTAAFGRAQLTKLDRHLAIQKENASALTEEL
                     KGTRGVILPYEPPNHTHNWYNYTIRLIPEEFGWKGKPHIFRNAVMKAIQAEGAQVGGW
                     QSFILPRMTVFQAKNAYGKGCPWSCPYSKSVEYDPDAYPVAQRHVDTHFGMTEPLRAP
                     NNVEVAKMVGKAIKKVIENIDSLDIEKLGTI"
     gene            complement(927..1928)
                     /locus_tag="PLW07_06045"
     CDS             complement(927..1928)
                     /locus_tag="PLW07_06045"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOL50123.1"
                     /translation="MSGRELVIKAVLFQKPERIPYTLPEEYGSDMTGDGVLPDPSWKP
                     SFPPSETKWEDEWHCVWERLSGDITKGQVTVHPLKDYQVFDSFQWPDFKIPERYKAIK
                     ECGINNKEDKFVLASIDFSLMHRLEYLRGHENAWTDPYLEPDNLNKLLDKLAELAIDQ
                     VDRVAEAGAHGIVSADDLGFQDRPMVSPEIFHNFFAPYYKKVYQHARKKGILCLLHSC
                     GYIIDLLDDLIDAGLQVIQMDQQENMGLENLDRKFGGRICFWCPVDIQKTMNSGTVED
                     VKNYARKLIDTFGKYDGGFIAKWYPSPDAVGHTKEKIKAMCETFISYGSQFYKNKKG"
     gene            complement(1928..2770)
                     /locus_tag="PLW07_06050"
     CDS             complement(1928..2770)
                     /locus_tag="PLW07_06050"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HOL50124.1"
                     /translation="MEFQKIILALEDYWIKKGCFLWHPYATEVGAGTMNPATFFGILG
                     KKKWNVVYLEPSQRPADARYGENPIRLYQHHQMQVILKPPPSNIQDVYIQSLKKLGID
                     ISTHDLRFIEDNWEAPTLGAWGVGWEVWLDGLEITQFTYMQQAGGIDLDPVSVEITYG
                     LDRIACFLQKKQSVFDIDWGSNTNYGFLHQERERQYSIFGFKEADANLLWTCFDGFEK
                     EAKSLLEKKLFYPAYDYILKLSHIFNLLEARGSISVSERTRIIGRIRTLANICASQYI
                     ERGN"
BASE COUNT          838 a          582 c          554 g          814 t
ORIGIN      
        1 caatgaatat agatacaaaa aaaattgaat ctgccattac agaaaaaaca aaggcgatta
       61 ttgtcgttca tcttcatggg ctcgctgtca atatggaaga ggttctttca atagcaaaaa
      121 aatacaattt gaaagtgatt gaagattgtt gccaggcgca tggagcagaa ttcaaaggta
      181 aaaaggttgg tctgtggggc gattgcgctg cgttcagttt taatcagaac aaatgccttt
      241 gctcaggtga aggcgggatg tttgttactg acaacgaaga aacttacaaa aaggcattga
      301 tgttatggtc ttttggtgaa acaagggcac cgtctgaaaa aagggattat cacgcctatg
      361 ctcttggatg gatgtacaga aataatgacc tgactgctgc ctttggcagg gctcaactga
      421 caaaactgga cagacacctt gcaattcaaa aagaaaatgc gtctgcattg acagaggaac
      481 tgaaaggaac cagaggtgtc atcctgccct atgaaccacc aaatcacaca cataactggt
      541 ataattatac aatacggctt atccctgaag agtttggatg gaaggggaaa ccacatatat
      601 tcagaaacgc tgtgatgaaa gccatacagg cagaaggcgc gcaagttggt ggctggcaga
      661 gctttatcct tccaagaatg acagtattcc aggcaaaaaa tgcgtatgga aaaggttgtc
      721 cctggtcatg cccatacagt aaatcagttg aatatgaccc tgatgcgtat cctgttgcgc
      781 agaggcatgt tgacactcac tttggaatga cagagcccct tcgtgcgcca aataatgtgg
      841 aagttgcaaa aatggtaggg aaggcaatta aaaaggtgat tgaaaatatt gactcccttg
      901 atattgaaaa acttggcact atttaactaa ccttttttat ttttgtaaaa ttgtgagcca
      961 taagaaataa atgtctcaca cattgctttg attttttctt tggtatgccc aacagcatca
     1021 ggcgacggat accatttggc aatgaatccg ccatcatatt tgccaaaggt gtcaatgagt
     1081 tttcttgcat aatttttcac atcctccact gtgccagaat tcattgtttt ttgaatatca
     1141 acaggacacc agaaacaaat tcttccacca aattttctgt caagattttc aagccccata
     1201 ttttcctgct ggtccatctg aatcacctga agacctgcgt caattaggtc atcaagaaga
     1261 tcaataatgt aaccgcagga atgaagaaga caaagaattc cttttttcct tgcatgttga
     1321 tacacttttt tgtaatatgg cgcgaaaaaa ttgtggaaaa tttcaggaga taccattggc
     1381 ctatcctgaa atccaaggtc atctgctgaa acaatcccgt gagcacctgc ttcagcaacc
     1441 ctatcgacct ggtcaatggc aagttctgcc agtttatcaa gaagtttgtt tagattgtct
     1501 ggttcaagat acgggtctgt ccaggcattt tcgtgtcccc tgagatattc aaggcgatgc
     1561 atgagagaaa aatcaatgct tgcgagaaca aatttatctt ccttattgtt tatgccacat
     1621 tcctttattg ccttatatct ttcaggtatt ttaaaatcag gccattgaaa tgaatcaaac
     1681 acctgataat ctttcaatgg atgcactgtc acctggccct ttgtaatatc ccctgaaagc
     1741 cgttcccaga cacaatgcca ttcgtcttcc cattttgtct cagatggagg aaacgatggt
     1801 ttccatgacg gatcaggaag aactccatca cctgtcatat cgcttccata ttcctctgga
     1861 agcgtgtaag gaatcctttc tggcttttga aaaagtactg cttttataac aagttctctt
     1921 ccactcatca attccccctt tcaatatact ggctggcgca gatatttgca agtgttctga
     1981 ttctcccaat gattcttgtc ctttcagaca cgctgattga accgcgcgct tcaagaagat
     2041 tgaagatatg agaaagtttc aaaatgtaat cgtatgctgg atagaaaagt tttttttcaa
     2101 gaagggattt tgcctctttt tcaaacccat caaaacatgt ccataaaaga tttgcgtctg
     2161 cctccttgaa accaaaaatt gaatactgtc tttccctttc ctgatgcaaa aacccatagt
     2221 tggtgtttga accccaatca atatcaaaaa cagattgttt cttttgaaga aaacatgcaa
     2281 tcctgtcaag cccataggta atttccacgc tgacaggatc aaggtcaatg ccacctgcct
     2341 gttgcatata ggtaaattgc gttatttcaa gtccatcaag ccagacctcc cagccaacac
     2401 cccatgctcc aagtgttggt gcttcccagt tgtcctcaat gaaacggagg tcgtgtgttg
     2461 aaatatctat gccgagtttt ttcaaacttt gaatataaac atcctgtatg tttgaaggtg
     2521 gcggcttgag aattacctgc atctgatggt gctggtaaag acgaattggg ttttcaccgt
     2581 atcttgcatc agcaggtctt tgagatggtt ccagatacac aacattccat ttcttttttc
     2641 caagaattcc aaaaaaagtt gctggattca tggttcctgc gccaacttca gtcgcatagg
     2701 gatgccacag gaaacagccc ttttttatcc agtaatcctc aagtgcaaga atgatttttt
     2761 gaaattccat gcaaataatt ataacata
//
LOCUS       DAORYU010000192         2786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_24127_length_2786_cov_3.157451, whole genome shotgun sequence.
ACCESSION   DAORYU010000192 DAORYU010000000
VERSION     DAORYU010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24127_length_2786_cov_3.157451"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1489
                     /locus_tag="PLW07_06055"
     CDS             <1..1489
                     /locus_tag="PLW07_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50125.1"
                     /translation="ELHNYSGNIKIRIEILDEDGSIKKSEKMLSIERSRTIFINNWEK
                     FVNSNEWQVTDNCLEFIPGKSISTIFIKPKVSGRFKVYFGLKYGIIHMMVAVSDEGIR
                     YPLVAERTRPEFQNKYHKEIFWKTVDFSTDTAIELSPTPMTVRQPDQYPFGSIAYIKL
                     VPTPERNYQITSLLWNDRVLALYFEPYSWAFCYGLDTKEKVKEALLLFKEMGANQVHT
                     QVIRFGSKALHHSRVAERYEHGAIMGDDGTFSPGPSVMVRALDVLKETSEICHNLGIT
                     HFANAGLTNCYPGTDFEERISRQHPEWRTGNILRYNIPETRKYVAEIIKEFVEWGNDG
                     VSIDCMRYPYYHTEEDLLKLFYEIRRAMDEVLPKRIPLTVRIPAGDIVYYTVFEQLAK
                     ENIIQCVIPSNLLTRQPLFSLKPYLKWKDYGCNVFGIIDGWLIHMASYFNFQLSLYRF
                     PSDIKRDIRRFFNEGADGIFVYQADLYCADPFTREVFNWRKWRKK"
     gene            1474..2310
                     /locus_tag="PLW07_06060"
     CDS             1474..2310
                     /locus_tag="PLW07_06060"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HOL50126.1"
                     /translation="MEKEIRIGTSGFSFSDWKGVVYSEKISASRMLEFYAKQLGFNAV
                     EINSTYYQMPSAKNFETMVKKTPQDFLFVVKAFKGMTHDPFDNRLQEKPDRYQVKQYF
                     LQFKDAIKPILENNKLGAVLMQFPVFFYPSKDSMEYILQAKQLLENVKLVVEFRNISW
                     ADEKYFDFLEKNNVALCAVDEPKIPRLMPLLDRVTSSVVYLRCHGRNPNWFNTPVSER
                     YNYNYSELELKEIERIARLMIQKAQVSFIFFNNCHAGYAAKNAVKFAELLDIPLRKEE
                     LF"
     gene            complement(2307..>2786)
                     /locus_tag="PLW07_06065"
     CDS             complement(2307..>2786)
                     /locus_tag="PLW07_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013387555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOL50127.1"
                     /translation="EGIKTSLSSTSLLVLAITLHNIPEGLAVGVAIGATSFGLADITM
                     TGALTLALGIGIQNLPEGFAVSISLRKEGVSRFKSFIYGQLSAIVEPIFAVIGTVGVL
                     WAKSFLPYAMGFAAGAMIFVVVEEIIPESQRHGNTDVSTAGTILGFLVMMILDVAFG"
BASE COUNT          921 a          494 c          557 g          814 t
ORIGIN      
        1 tgaactgcac aattattccg gtaatataaa aattcgtatt gaaattcttg atgaagacgg
       61 ttcaataaaa aaatccgaaa aaatgctgtc tatcgaacgt tcccgtacaa tttttatcaa
      121 taactgggaa aaatttgtaa atagtaatga atggcaggtt acagataatt gccttgaatt
      181 tattccagga aaaagtattt caacaatttt tatcaagcct aaagtttcag gaagatttaa
      241 ggtgtatttt ggattgaaat acgggattat ccacatgatg gttgccgtgt cagatgaagg
      301 aataagatat cccttggttg ccgaaagaac taggcctgaa tttcagaata agtatcacaa
      361 ggaaattttc tggaaaactg ttgatttcag tactgataca gccatagaac tgagtccaac
      421 tccgatgact gtcaggcaac cagaccaata tcctttcggt tcaattgctt acatcaaact
      481 tgtgcctaca ccagaaagaa attatcaaat aacgtctctt ctgtggaatg acagggttct
      541 cgcactctat ttcgaaccat attcctgggc gttttgttat ggacttgata ccaaagaaaa
      601 agtgaaagaa gcactgcttt tgtttaagga gatgggagca aaccaggttc atactcaggt
      661 tataaggttt ggctcaaaag cgcttcatca cagtagagtg gcagaaaggt atgaacatgg
      721 cgcaataatg ggagatgacg gaacattcag tcctggacca tccgtaatgg ttagagccct
      781 tgatgtcctg aaagaaacaa gcgaaatatg ccataatctt ggaattaccc attttgcaaa
      841 cgccgggctt acaaactgtt accctggcac tgattttgaa gaaagaattt cgaggcaaca
      901 ccctgaatgg agaactggaa atatccttag atataacatt ccagaaacaa ggaaatacgt
      961 agctgaaatt attaaggaat ttgttgaatg gggaaatgac ggtgtttcca ttgattgtat
     1021 gaggtatcct tattatcata ctgaagagga tcttttgaaa cttttttatg aaataagacg
     1081 cgcgatggat gaagttttac ctaaaagaat tcctctaaca gtgagaattc ctgctggaga
     1141 tatcgtatac tacacagtgt ttgaacaact tgcgaaggaa aatataattc agtgtgttat
     1201 cccatcaaat cttttaacgc gccagccgct tttttctttg aaaccatatt tgaaatggaa
     1261 ggattatggt tgcaatgttt tcggaattat tgatggctgg ctgattcaca tggcgtcata
     1321 tttcaatttc caactttctc tttacaggtt cccttcagac ataaaaagag atatcagaag
     1381 attttttaat gagggtgcag atggaatatt tgtttaccag gcagatttat actgcgctga
     1441 tccttttacg agagaagtat ttaactggcg aaaatggaga aagaaataag aattgggaca
     1501 agcggatttt cgttttctga ctggaaaggt gttgtgtatt ctgaaaaaat ttctgccagc
     1561 agaatgcttg aattttatgc aaagcaatta ggtttcaatg ctgtcgaaat aaactccacg
     1621 tattatcaga tgccttctgc aaaaaatttt gaaacaatgg tgaagaaaac cccgcaggat
     1681 tttttatttg ttgtcaaagc atttaaaggt atgacccatg atcctttcga taatcgtctg
     1741 caggaaaaac cagacagata tcaggtgaaa caatattttc ttcaattcaa ggatgcaata
     1801 aaaccgatat tagaaaacaa caaacttggc gcagtactca tgcagtttcc tgtatttttt
     1861 tatccgtcga aagattcaat ggaatatatt ttacaggcaa agcaactttt agaaaatgta
     1921 aaactggtcg tagaatttag gaatatctca tgggctgatg agaaatattt cgattttctt
     1981 gagaaaaata atgttgcact gtgcgcggtc gatgaaccaa aaattccccg ccttatgcca
     2041 ctattagata gagtaacttc ttctgttgta tatttgcgat gccatggaag aaatcccaac
     2101 tggtttaata cgcctgtttc tgaacggtat aattacaatt attctgaact ggaattaaag
     2161 gaaattgaaa ggattgcccg actgatgatt caaaaagctc aggtgagttt tatttttttc
     2221 aataactgcc atgcaggata tgctgcaaaa aacgctgtga agtttgcaga attgcttgat
     2281 attcctttaa gaaaagagga attgttttaa ccaaaggcaa cgtcaagaat catcatgacc
     2341 agaaaaccca gaattgttcc agcggttgat acgtcagtat taccgtgcct ctgcgattca
     2401 ggaatgattt cctcaacaac aacaaaaatc attgctcctg ccgcaaagcc cattgcataa
     2461 ggcaaaaaag attttgccca caacacgcca actgttccaa tgactgcaaa aataggttca
     2521 acaattgcgg aaagctgacc atagataaaa cttttgaatc tcgaaacccc ttcttttctt
     2581 aaagaaattg aaacagcaaa accttctggc aggttttgaa taccgatacc cagtgcgagt
     2641 gttaatgcgc ctgtcattgt aatatcagca agtccgaagg atgttgcacc gattgcaaca
     2701 cccacagcca gaccttcagg gatattatga agagtaatag caagcaccag aagtgatgtg
     2761 gaacttaaag atgttttgat tccttc
//
LOCUS       DAORYU010000193         2753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_24538_length_2753_cov_4.383617, whole genome shotgun sequence.
ACCESSION   DAORYU010000193 DAORYU010000000
VERSION     DAORYU010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24538_length_2753_cov_4.383617"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1807)
                     /locus_tag="PLW07_06070"
     CDS             complement(<1..1807)
                     /locus_tag="PLW07_06070"
                     /inference="COORDINATES: protein motif:HMM:NF027900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HOL50128.1"
                     /translation="MKKIIFLLPVLFSGILSYGLPQRILSNFEENTGSWSVIEGSTAV
                     CLMEQSSQNATEGYGCLKCTVNFPGETGIKMLLNENWTGYQSLMFDMIVTETPIEQIK
                     FFVYIKDKEWLWYQTKPQPVKYGTTKVAINISGSSLDLIPAGHKKPWNQYSTDQVREI
                     GIKFLSDAKITQSFYIDNVRLSPVLFSSLKVNRTEVPVYEKFEISFKTPVYFSNPFDP
                     DCIAIDGYFVSPSGKEIVVPGFFYQDFYFAGPGTKGQDNLQPQGLPEWRIRFSPLEKG
                     TYKYKIVASINKGEETFSTHTMTFRAIDSKSSGFVRVSKKDNRYFEFDNGEFFYPIGH
                     NIRSLNDNRYSQLFHRPLAAQDGTINFDTWLNDMEKNKENFFETWMAAWWIAIEWKKG
                     YGFYEGLGRYDLRNAWKLDWILDLASKKGIYIQLLIVNHGSVSTYCDQEWQDSPYNIK
                     NGGFLNSPEEFFTSQQAKDLFKKRLRYIVARWSYSPNIFSWELVNEMNLIGASGNFYK
                     TPVLAAWYKEIGEYLSQIDPNDHMITGHYTILYDSDVFKLPQVDYVLTNAYYDVRGGN
                     IVDYLKVVANFNARFNKPHFVSEYGGNWNAGPEALI"
     gene            complement(1824..2228)
                     /locus_tag="PLW07_06075"
     CDS             complement(1824..2228)
                     /locus_tag="PLW07_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOL50129.1"
                     /translation="MDAKGMGSLISGTKGFTLIELLMVVIIVGLLAAIAIPIYRVNIK
                     KVASSEGAALLGTILTAQKVHYSEHNIYTSDENVLMIDLAGNKYFRDFEITHADADGF
                     TAKTSGTSICDGIEVTMVYTNIAGATISYSGF"
     gene            complement(2201..>2753)
                     /locus_tag="PLW07_06080"
     CDS             complement(2201..>2753)
                     /locus_tag="PLW07_06080"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOL50130.1"
                     /translation="DNLGLSEDTMLVFTTDHGHLIGQHNLAAKGAFHYEDLLRVPFIV
                     RWPGRVPENKISDALISFVDFAPTILSLCGLKIPRTMSGIDQSSVFCDKVESLRNWVL
                     VENRHEPTTIFLKTYIEKRYKITVYFRKDYGELFDLENDPHEYNNLWNEPEYRERKNQ
                     LIKKLLFAEWEKEPLWMPRVWGA"
BASE COUNT          835 a          579 c          471 g          868 t
ORIGIN      
        1 caatcagtgc ttctggtcct gcgttccagt ttccaccata ttcagaaaca aaatgtggtt
       61 tgttaaacct tgcattaaag tttgccacca ccttgagata atcaacgata ttacctcccc
      121 tcacatcata ataagcattt gtaagaacat aatcaacctg cggaagtttg aaaacatcgc
      181 tatcatacaa aatcgtataa tgccctgtaa tcatgtggtc attcgggtca atctgtgaaa
      241 gatattctcc aatttcttta taccatgcag caagcacagg agttttataa aaatttccac
      301 ttgcgcctat caaattcatt tcattgacaa gttcccatga aaaaatattt ggactgtatg
      361 accatcgtgc aacaatgtat ctgagccgtt ttttgaataa gtcctttgcc tgttggctgg
      421 taaaaaattc ttcaggtgaa ttcaaaaatc cgccgttttt gatattgtag gggctgtcct
      481 gccattcctg atcacaataa gtactcactg aaccatgatt aacaatcaac aattgaatat
      541 aaatcccttt ttttgaggca aggtcaagaa tccagtcaag tttccaggca tttcttaaat
      601 catacctgcc aagaccttca taaaaaccgt atcccttttt ccattcaatt gctatccacc
      661 atgctgccat ccatgtttcg aaaaagtttt ctttgttttt ttccatatca ttaagccagg
      721 tgtcaaaatt gattgtccca tcctgggcag caagtggacg atgaaatagt tgagagtatc
      781 tattgtcatt gagagaccgt atgttatgcc cgattgggta aaaaaactca ccattatcaa
      841 attcaaagta tctgttgtct tttttgctta cacgaacaaa tcctgaactt tttgaatcaa
      901 tcgccctgaa ggtcatagtg tgagtagaaa acgtttcttc acccttgttt atggatgcca
      961 ctattttata cttataggtc cctttttcca gaggagaaaa ccgtattctc cattcaggca
     1021 atccctgtgg ctgaaggtta tcctgccctt tggtgccagg acccgcgaaa taaaaatcct
     1081 gataaaaaaa accaggtacc actatttcct ttcctgatgg cgagacaaaa tatccgtcaa
     1141 tggcaataca atcagggtca aaaggatttg aaaaataaac tggtgttttg aacgatattt
     1201 caaacttttc ataaacaggc acttctgtcc tgttaacctt caaagaagaa aacaatactg
     1261 gagaaagtct gacattgtca atataaaatg actgggtaat ctttgcgtca gataaaaact
     1321 ttataccgat ttccctgacc tggtcagtac tgtactggtt ccagggtttt ttatgcccgg
     1381 caggaattaa atcaagactt gaaccagaaa tattgattgc aacttttgtc gtaccatatt
     1441 taacaggttg tggttttgtc tggtaccata accactcttt atctttgata taaacaaaaa
     1501 attttatctg ctcaattggt gtttcagtga caatcatatc aaacatcagg gattgataac
     1561 cggtccagtt ttcgttcaga agcattttta ttcctgtttc accaggaaaa tttaccgtac
     1621 atttaaggca tccgtaacct tctgttgcat tctgactgga ttgttccata agacaaacgg
     1681 ctgtagaacc ttcaataaca ctccaggagc cagtgttttc ttcaaaattg gaaagaattc
     1741 tctgcggcag gccataagaa agtatgcctg aaaaaagcac tggcaataaa aaaattattt
     1801 ttttcactgt gaattaccct gaatcaaaat cctgaataag aaatggttgc acctgcgata
     1861 ttggtataaa ccattgttac ttcaattcca tcgcagatac tggtaccaga tgtttttgct
     1921 gtaaacccat ctgcgtctgc atgtgttatt tcaaaatccc tgaaatattt gtttcctgct
     1981 aaatcaatca taagcacatt ttcatctgaa gtataaatat tgtgctctga ataatgaacc
     2041 ttctgggctg tgagtattgt gccaagaagt gctgcacctt cgcttgaagc aacctttttg
     2101 atatttaccc tgtatatcgg tattgcaatt gctgcaagca gtcccacaat aatcaccacc
     2161 attaaaagtt caatcagggt aaatcccttt gtgcctgaaa tcaggctccc catacccttg
     2221 gcatccatag tggctccttt tcccattcag caaaaagaag tttttttatc aactggttct
     2281 ttcgttctct gtattcaggt tcgttccaca aattattata ctcatgcggg tcattttcaa
     2341 ggtcaaaaag ttctccgtag tcctttctga aatatactgt aatcttgtat cgtttctcaa
     2401 tatatgtttt cagaaaaatc gttgttggtt catgcctgtt ttcaacaaga acccagtttc
     2461 ttaaagattc cactttgtcg caaaaaacag aactctggtc aataccactc atggtgcgcg
     2521 gaattttgag tccgcataaa gaaagaattg tcggcgcaaa atcaacaaat gaaatcaaag
     2581 catcagaaat tttgttttca ggaacacggc ctggccatct tacaataaat ggaacacgca
     2641 acaaatcttc ataatgaaac gctcctttcg cagcaagatt gtgctgaccg atgagatgac
     2701 cgtggtcagt agtaaaaact aacatggtat cttctgaaag tccaaggtta tca
//
LOCUS       DAORYU010000194         2750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_24568_length_2750_cov_4.469388, whole genome shotgun sequence.
ACCESSION   DAORYU010000194 DAORYU010000000
VERSION     DAORYU010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24568_length_2750_cov_4.469388"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="PLW07_06085"
     CDS             complement(<1..968)
                     /locus_tag="PLW07_06085"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOL50131.1"
                     /translation="MEAKETLQYEIYLLDAICRGLQATLNVDKIVHIILTGLTAGASL
                     GFSRAAIFFINETGILHGGRGIGPYDQNEAGQIWKDLTNSDVALTEMFDNSHRRTLES
                     QRFPVEIKSIRYEIAKLPASSPLKKVIDANETVLLKEDERFLVPDEFHWFVRYATQVV
                     IAPITISGKISAVVFADNAFHYKEITPATISFLSIVLNQAGLALSNAFAYEATRHNLQ
                     QVQHLNEKLRQVQEELLACERFAAAGRISTYLAHEIRNPLATIGGFARQILEISREKS
                     SDPRIARNARIIANEVRRLELVLNNLLRFSFKQPAKKQNIHLLSFL"
     gene            complement(971..1672)
                     /locus_tag="PLW07_06090"
     CDS             complement(971..1672)
                     /locus_tag="PLW07_06090"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOL50132.1"
                     /translation="MKWFRKKRNFRMVMIVTAALVIPGFIIWGVSISGTDKRYLVATV
                     NKEPIYQHEFYKTLEEIRQQYRDILKDNYAKLINETELEKMVLNRMIQEKLLEQLYRR
                     HHIRVRDSEILEVIKSDPSFKNEKGQFDEAKFRAYFSRISPEKTKEIEDQIRNSIKYQ
                     KLKQEALSETTIVVSDQEVKSLMGDKMDENKFQDIKRYVQSQKEDQYFQQWLGKQKDR
                     AKIEIFVNLDRKTNS"
     gene            complement(1672..2340)
                     /gene="rsmI"
                     /locus_tag="PLW07_06095"
     CDS             complement(1672..2340)
                     /gene="rsmI"
                     /locus_tag="PLW07_06095"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOL50133.1"
                     /translation="MLFIVATPIGNLEDITFRALNVLKTVDFVVSEDTRETRKLLNYF
                     QIQKPLLSYYRGCEKSRSHQIMKLLNEGKNVALVCDRGTPGISDPAHYIVRRCLEEGI
                     QVSPVPGPSALMAGLSVSGEILDAFSFYGFLPRKKNKKLEFFKNMGEREETIVFFESV
                     HRFLETLEILNQLFPERRLVVCRELTKKFEDIVAGSVNQMYNKFKQTTVKGEFVLILG
                     GKQC"
     gene            2474..>2750
                     /locus_tag="PLW07_06100"
     CDS             2474..>2750
                     /locus_tag="PLW07_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244994.1"
                     /note="catalyzes the interconversion of 3-phosphoglycerate
                     and 2-phosphoglycerate; this enzyme does not require the
                     cofactor 2,3-bisphosphoglycerate as a phosphate donor;
                     BPG-independent PGAM; aPGAM; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HOL50134.1"
                     /translation="MEFEKILKNVIKKNENKILLLVMDGLGGLPHPETGLTELETAKK
                     PNLDSIAKSGMCGLSIPVLPGITPGSGPAHLSLFGYDPIAHQIGRGVL"
BASE COUNT          731 a          580 c          516 g          923 t
ORIGIN      
        1 ttgaggaaac tgagaagatg aatattttgc ttctttgccg gctgtttgaa tgaaaacctc
       61 aggaggttgt tcaatacaag ttcaagcctt ctcacctcat tggcaataat tcttgcattt
      121 ctggcaatcc ttggatcact gcttttttct ctggatattt caagaatctg tctggcgaac
      181 cctcctatgg ttgcgagtgg attccgaatt tcgtgcgcaa gatatgtgga aattcttcct
      241 gcagcagcaa atctttcgca tgccagtagt tcttcctgga cctgtctgag tttttcgttt
      301 aagtgttgta cctgctgcag gttgtgcctt gttgcttcgt aagcaaaggc attactcagg
      361 gcaaggcctg cctgattgag cacaattgaa agaaatgata ttgttgcagg agtgatttct
      421 ttataatgaa aggcattgtc agcaaacacc acagcagaaa ttttccctga aatggtaata
      481 ggggcaatca ccacctgcgt tgcatacctt acaaaccagt ggaattcatc aggaacaaga
      541 aacctctcat cctctttcag taatacagtt tcgttagcat caatcacctt tttcaaagga
      601 ctgctggctg gaagttttgc aatttcatat cggatggatt tgatttccac agggaatctc
      661 tggctttcaa gggttcttct atggctattg tcaaacattt ctgtcagcgc cacatcagag
      721 tttgttagat ctttccatat ctggcctgct tcattctggt cataaggacc tatacccctt
      781 ccaccatgca ggataccagt ttcattgata aaaaatatcg ctgctctgct gaaaccaagg
      841 gaagcacctg ctgtgagtcc tgtcaggata atatggacta ttttgtcaac atttagtgtt
      901 gcctgcaggc ctctgcatat cgcgtccaga agatatattt cgtattgaag ggtctctttt
      961 gcttccattt ttatgagttt gttttcctgt caaggttaac aaaaatctca atttttgccc
     1021 tatctttttg ttttccaagc cactgctgaa aatactggtc ttctttctgt gactgaacat
     1081 atcgtttgat atcctggaat ttattttcat ccatcttatc acccatcagg gactttactt
     1141 cctggtctga taccacgatc gttgtttctg aaagggcttc ctgtttaagt ttctggtatt
     1201 taatgctatt tcttatctga tcttcaatct cttttgtttt ttcaggagaa atacgtgaaa
     1261 aatacgccct gaattttgct tcatcaaact ggcctttttc atttttgaat gatggatcag
     1321 attttatcac ttcaagaatt tctgaatctc ttaccctgat gtgatgcctg cgataaagct
     1381 gctcaagcaa tttttcctgt atcatcctgt taaggaccat tttttcaagt tctgtttcat
     1441 tgatgagttt tgcataattg tccttcaaaa tatctctgta ttgctgtcgt atttcttcaa
     1501 gagttttata aaattcgtgc tgataaatcg gttctttgtt cactgttgcc acaagatatc
     1561 gtttatcagt gcctgaaatg cttacacccc atatgataaa gcccggaatt acaagtgctg
     1621 cggtcacgat catcaccatt ctaaaatttc tttttttcct gaaccatttc atcaacattg
     1681 ttttcctccg agaatgagaa caaattcacc ttttactgtg gtttgtttaa atttattgta
     1741 catctggttc acactgccag caacaatgtc ctcaaatttt tttgtcagtt ccctgcacac
     1801 aacaagccgt ctttcaggaa acagttgatt gagaatctca agtgtttcca gaaacctgtg
     1861 aacagattca aaaaaaacaa tggtttcttc tctttctccc atgtttttga aaaattcaag
     1921 ttttttattt ttctttcttg gcaagaaccc ataaaaagag aaagcatcca ggatctctcc
     1981 ggaaactgac agaccagcca ttaaagcaga aggtcctggt acaggggaca cctgtatgcc
     2041 ttcttcaagg catcttctta cgatatagtg agcagggtct gaaattcctg gtgttcccct
     2101 gtcgcataca agcgcaacgt ttttgccttc gttcaataat ttcataatct gatgagacct
     2161 gcttttttca caccccctgt aataactgag tagaggtttt tgaatctgaa agtaattgag
     2221 caatttcctt gtttctcttg tatcttctga aacaacaaaa tcaacagttt tcaacacatt
     2281 cagtgccctg aaggtaatgt cttcaagatt gcctatggga gttgccacta taaaaagcat
     2341 aataattatc cttttttcgg aattaagtaa aaaagagagt caagtccgca agttttgaca
     2401 tttccgcata tttatagtac cctaaaatga agaaattatc aatctttctg aggtccaaat
     2461 ccggggttca accatggaat ttgagaaaat attaaaaaac gtcatcaaga aaaacgagaa
     2521 taagattctc ttgcttgtaa tggatggtct gggaggcctg ccgcatcctg aaaccggtct
     2581 tacagaactt gaaacggcaa aaaagcccaa tcttgattca attgcgaaat caggtatgtg
     2641 tggattgtca attcctgtgc tgcctggtat tacgccaggc agtggtcctg cgcatctgtc
     2701 cctgttcgga tatgacccca ttgcacatca gataggcagg ggtgtgcttg
//
LOCUS       DAORYU010000195         2742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_24690_length_2742_cov_4.953108, whole genome shotgun sequence.
ACCESSION   DAORYU010000195 DAORYU010000000
VERSION     DAORYU010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24690_length_2742_cov_4.953108"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="PLW07_06105"
     CDS             complement(<1..999)
                     /locus_tag="PLW07_06105"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOL50135.1"
                     /translation="MIIQYSNLDDSKKIFLNRELADILYLCRKRKISVPEFDINVVSL
                     SEYNQIPEHLQSKISIKIPSKNKHFSSFNQMCEYENDIQTSQVIRDENIIWIIGAGSE
                     GALYGFYETIRKITGIRWYGTSEFDMAFAKPNSAIEGIYRPRVPLRGFEFSPKNENHE
                     FSEKFLKWMVRNRWNLLDINVSHWEKYANKQEFIRLCNTFGIRLAIGCHAVDFFVPEF
                     IYNKTPHMFGVRDGNRCITSTVGSPDIKKRWQTKIQPCYTNSETRELYVNAVVDFINN
                     HPETYIFSLWPHDGINNWCQCQNCKEQSPYEMMYRIALEILEKLGTRILILDDVATY"
     gene            1259..>2742
                     /locus_tag="PLW07_06110"
     CDS             1259..>2742
                     /locus_tag="PLW07_06110"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDC27 family protein"
                     /protein_id="HOL50136.1"
                     /translation="MSKKILLFMLAFLLPGMMFCQEKKVELKDIIKAFQKNEFSLTVE
                     LANRAIKEGLEHPDLYFYLGMAYSKIGKNQEAIQIFRIFLNRADYSKNPWMLRQAFQN
                     LINIHKATKNFESIIEDGNFFLDKIRAVQNTEQLEVFCKNIIVEALKEIGNQKGNNND
                     FAGAIEAYKKALEYKPDDSSSCERIATYYKNLGRNELATEYFLKSATLGTAWSSKVWP
                     LISAAELIWESGKFEEFAGKVENDVLSYNVMLAMGELKNHRYQDAFLRMKKAEEIVGS
                     KGNVSERILKSWYRWFREDASFFYSFIVVFPEHTSVSWAIDMITSIARTNTESAKLIR
                     EKITPSIVELYEKNRESSDFKKLFSRLVDLKFAGIPETPETAREKIQMYEEFKKGITD
                     EALIRDIIKREASLYTDVLANYSKGTELYELLVNKYKDKGAIQPLARCLINLEQFERA
                     YQILKEYVSDKGIGEYARFQAAQLLIQANFFDEGIRVLNEIVSST"
BASE COUNT          886 a          444 c          552 g          860 t
ORIGIN      
        1 gtaggtggct acatcgtcca aaattaaaat acgggttcca agtttttcta agatttcaag
       61 ggcaatgcga tacatcatct cataaggcga ttgttcttta caattctggc attgacacca
      121 gttgttgatt ccgtcgtgtg gccagagaga aaaaatataa gtttcaggat gattgttgat
      181 aaagtcaaca acagcgttaa cataaagttc acgcgtttca gaatttgtat aacatggctg
      241 tatctttgtt tgccatcttt ttttgatatc aggagagccc actgtggatg tgatacagcg
      301 atttccatct cgcacaccga acatgtgcgg cgttttgtta taaataaatt caggaacaaa
      361 aaaatcaaca gcatgacatc cgatggcaag tcttatcccg aatgtattgc acaatcttat
      421 aaattcctgt ttgtttgcat atttttccca atgagaaaca ttgatgtcaa gaagattcca
      481 tctatttctg accatccatt tcaagaattt ctctgaaaac tcatgatttt catttttggg
      541 cgagaactca aaacccctta gaggaactct tggcctgtat attccttcga ttgctgagtt
      601 tggttttgca aatgccatat caaattcact ggtaccatac cacctgatac cagtgatttt
      661 tcttattgtt tcatagaaac cgtatagggc tccttctgaa ccagcgccga taatccagat
      721 aatattttca tccctgatta cctgacttgt ttgaatatcg ttttcgtatt cacacatctg
      781 gttgaaactg ctgaaatgct tattttttga aggaatcttg atggagattt tgctttgtaa
      841 atgctctggt atctggttat actctgataa agagacaaca ttgatatcga attccggaac
      901 agagattttt cgctttctgc acaggtacaa aatatctgca agttccctgt taagaaatat
      961 tttcttgctg tcatcaagat ttgaatattg tatgatcatt tcaggtcctt tttaatatca
     1021 taccagagta acagacgtgt ttcaacaata gacaatttac ttctttttac gtcaaattga
     1081 cagaagcatt ccatatagca aacgttaata tggtaagata ctatagtgca aaaacctgga
     1141 ttaaaaaggt tgataattgg gtgaacctat aaaaagatgt tctctgaatt tgcatgttaa
     1201 acccttacgt tgcggtttga gttgaataaa gccgaaattt tcggctatga gtgggagaat
     1261 gagtaaaaaa atcttactgt tcatgttagc tttcttgctt cccggcatga tgttttgcca
     1321 ggagaagaag gttgaattaa aggacattat caaggcgttt cagaaaaacg aatttagttt
     1381 gacggttgaa cttgcaaata gggcaattaa ggaaggttta gagcatcctg atttatattt
     1441 ttatcttggt atggcgtatt caaaaattgg caaaaatcag gaggcaattc aaatattcag
     1501 gatatttctc aacagggctg attattcaaa aaatccctgg atgttaagac aggcatttca
     1561 gaatcttatt aacattcaca aagccaccaa gaattttgag tccattattg aggatggtaa
     1621 cttttttctt gataaaataa gggctgtgca aaacacagaa caacttgaag ttttctgtaa
     1681 aaatattatt gtagaagccc tgaaagaaat aggaaatcaa aaagggaata acaatgattt
     1741 tgctggagca attgaggcgt ataaaaaggc gctggaatat aaaccggatg actcttcatc
     1801 ctgtgaaagg attgccacat attataaaaa tcttggcagg aatgaacttg caaccgaata
     1861 ttttttgaag tcagcaactc tcgggactgc ctggtcgagc aaggtctggc cacttatttc
     1921 tgctgctgaa ttgatatggg aatcaggaaa atttgaagag tttgcaggaa aagtggaaaa
     1981 cgatgtgctt tcgtacaatg ttatgcttgc gatgggtgaa ttgaaaaatc acaggtatca
     2041 ggatgcgttt ttgagaatga aaaaagcaga agagatcgtc ggttctaagg gaaatgtatc
     2101 agaaagaatt ttgaagagtt ggtacaggtg gtttagagaa gatgcaagct ttttctattc
     2161 ttttattgtt gtttttcccg agcacacatc agtatcatgg gctattgata tgataacaag
     2221 tattgcaagg acaaatactg aatctgcgaa gttgataaga gaaaaaatta ctccatcgat
     2281 tgttgaactt tacgaaaaaa acagggaatc atcggatttc aaaaaacttt tttccagact
     2341 tgtggacttg aaatttgcag gtattcctga gactccggaa acagccagag aaaaaattca
     2401 aatgtatgaa gaattcaaaa aaggaatcac tgatgaggca ctaattcggg acattataaa
     2461 aagagaagca tcgctttata ctgatgttct tgctaattat tcaaagggaa cggaacttta
     2521 tgaattgctt gttaacaaat acaaagacaa aggcgcaatt caaccgcttg ctagatgttt
     2581 gataaatctt gagcaatttg aacgggcata tcaaattctt aaggagtatg tttctgataa
     2641 gggcattgga gaatacgcca gatttcaggc agcacaactt ttgattcagg cgaatttttt
     2701 tgatgaaggt attagagttt tgaatgaaat agtttcatct ac
//
LOCUS       DAORYU010000196         2739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_24724_length_2739_cov_4.283905, whole genome shotgun sequence.
ACCESSION   DAORYU010000196 DAORYU010000000
VERSION     DAORYU010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24724_length_2739_cov_4.283905"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="PLW07_06115"
     CDS             complement(<1..873)
                     /locus_tag="PLW07_06115"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOL50137.1"
                     /translation="MQENPEEHKKLFKKTKDFLVAIDTDGCIVDNMNAKQMLIFHPLY
                     MEFYQLWKIESYFREVAEFYNLFSVHRGCNRFIAIRLILMALNSRKDVQSQVEKMNVV
                     VPNIEPVDQYIEFCRNNNSGMGNISLSQFISTRPLDFELYKLSGWSHAVNKALDVIVD
                     KIQPFENVRECLEMIYKHADIAVVSQTPYQDLFNYWDTHDLLKYVKIICGQEMGSKTY
                     HIEILKKIGKYSDEQVLMIGDAPGDLDAIKKNNGFFYPVTPGIENDSWKQFSEAFGLF
                     LQGKYHVEMEQHLID"
     gene            complement(948..2435)
                     /gene="gndA"
                     /locus_tag="PLW07_06120"
     CDS             complement(948..2435)
                     /gene="gndA"
                     /locus_tag="PLW07_06120"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004889642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HOL50138.1"
                     /translation="MKKANIAIIGMEVMGRSLALNMESKGYTVAVYNRTAEKTKAFAE
                     GPARGKNIIPTYELKDLVDSLESPKKIMLMVKEGQPVDNFIETLLPYLKTGDLIIDGG
                     NSFFKDTIRRNKMLSEKGILFIGTGVSGGEEGALKGPAIMPGGQKEAYNLVEKIFRDI
                     SAKAYDGEPCVDYIGPDGAGHFVKTIHNGIEYGDMQLIGEVAWFFKNIGMRADEIAEI
                     MAQWNGKDDILRSYLIEITADSMKQKHQGSDEFLVDRVADITRMKGTGTWTVQSALEL
                     LVPIPTIAAAVFSREMSQDKQLRLSMAKKITIASGKFNGDKKSIVEIAHDALYIAKIS
                     SYAQGIALLQAASKEYNFNLNIGRIAKIWRAGCIIRAQFLDEITKAYEAEPDLPNLLA
                     APKFASFVETNAGKLAKFIDIAHHGGVPVLAMSGALDYIMQITSPVMPSAQVSALQRD
                     YFGAHGYFKIGGPDDPELVTNEEGKVREFHTEWLLPGRPEREITK"
     gene            complement(2461..>2739)
                     /locus_tag="PLW07_06125"
     CDS             complement(2461..>2739)
                     /locus_tag="PLW07_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOL50139.1"
                     /translation="AVHFAMEYNKLLRVNAIAPGFFLTQQNRYLLTDEKNNLTPRGQT
                     IINHTPMGKFGEPDDLIGTCIWLASKASKFVTGVVVPVDGGFNAYSGV"
BASE COUNT          776 a          614 c          451 g          898 t
ORIGIN      
        1 atcaatcaag tgttgctcca tttccacatg gtacttaccc tgtaaaaaca atccaaacgc
       61 ctcagaaaat tgtttccagg aatcattttc aattccaggt gtaacgggat aaaaaaaacc
      121 attatttttt ttgatggcat caaggtctcc aggggcatca ccaatcataa ggacctgttc
      181 atcagaatat ttgccaattt tcttcaaaat ctctatatgg tatgttttgc ttcccatttc
      241 ctgaccgcag ataattttca catatttcaa aagatcatgg gtatcccagt aattgaataa
      301 gtcctggtac ggtgtttgag aaactacagc aatatccgca tgtttgtata tcatttcaag
      361 acattctctc acgttttcaa atggctgaat tttatcaacg atcacatcaa gcgctttatt
      421 aactgcatga ctccatcctg aaagtttata aagttcaaaa tcaagtggtc ttgttgaaat
      481 aaactggctc aacgaaatat ttcccatacc agaattgttg tttctgcaaa attcaatata
      541 ctggtcaaca ggttcaatgt tcggcacaac cacattcatt ttttctactt gggattgaac
      601 atccttccga ctgttcaaag ccatcaatat caatctgata gcgataaacc tgttgcaacc
      661 tcggtgaaca gaaaaaagat tataaaattc agcaacctct ctaaagtagg attcaatctt
      721 ccacagttga taaaattcca tgtataacgg atgaaatatc agcatctgtt tggcattcat
      781 gttgtcaaca atacatccat ccgtatcaat tgccacaaga aaatcttttg ttttcttgaa
      841 caacttctta tgctcttctg gattttcttg caattttcgg cctgcaggtt tttcaaacat
      901 atgcaatagt aaaaccagtg atttgcaccc ggaatattaa atctcattta ctttgtaatt
      961 tccctttcag gtcttccggg aagcaaccat tcagtatgga actcgcgcac ctttccttct
     1021 tcatttgtaa caagttctgg atcatcaggc ccaccaattt taaaataacc gtgggctccg
     1081 aaataatctc gctgaagagc agaaacctgg gcagacggca tcacaggtga cgtaatctgc
     1141 attatgtaat caagtgcacc agacatagcc agaacaggaa ctccgccatg gtgagcgata
     1201 tcaataaatt ttgcaagttt tcctgcattg gtctcaacaa aactggcaaa ttttggtgct
     1261 gcaagaagat tgggaagatc cggttcagct tcatatgcct tggtaatttc gtcgagaaat
     1321 tgagccctga tgatacatcc cgcacgccat atttttgcga ttctgcctat attgaggttg
     1381 aaattgtatt cttttgatgc agcctgaagt aaagcaattc cctgagcata ggatgaaatt
     1441 ttggctatgt acagagcgtc gtgggctatt tcaacaattg actttttatc tccattgaat
     1501 ttgcctgacg caattgtgat tttttttgcc atggatagac gtaattgttt atcctgagac
     1561 atctctcttg aaaacactgc tgcagcaatt gttggaatcg gcaccagtag ttcaagagcg
     1621 ctttgaacag tccatgttcc agtgcctttc attctcgtaa tgtcagcgac cctgtcaaca
     1681 agaaattcgt cagaaccctg atgtttctgt ttcatgctat cagcagtgat ttctatcaaa
     1741 tatgatctca aaatatcatc cttaccattc cattgtgcca taatttcagc aatttcgtct
     1801 gctctcattc caatgttttt aaaaaaccac gcaacctcac caatcaactg catatccccg
     1861 tattcaattc cattatgaat ggttttcaca aagtgacctg ctccatcagg ccctatgtaa
     1921 tcaacacatg gttcaccatc atatgccttt gctgaaatat ctctgaagat tttctccaca
     1981 agattgtatg cttctttctg cccaccaggc ataatggcag gccctttcaa cgcaccttct
     2041 tctcctcctg aaacgcctgt cccgataaaa agaattcctt tttctgacaa cattttattt
     2101 cttctgattg tatccttaaa aaatgaattg cccccatcaa taataaggtc ccctgttttc
     2161 agataaggga gaagcgtttc gatgaaatta tctactggtt gtccttcttt taccatcagc
     2221 ataatctttt ttggactttc cagagaatca accagatcct ttaattcata ggtaggaata
     2281 atattttttc ctctggcagg accttctgca aacgcttttg ttttctctgc tgttctatta
     2341 tagactgcaa cagtataacc tttgctttcc atattgaggg caaggcttct tcccatgact
     2401 tccattccaa ttatcgctat atttgctttt ttcatttttt gctctccttt tttgcaaaat
     2461 ttaaacccct gaatacgcgt tgaatcctcc gtccacagga accacaacgc ctgtaacaaa
     2521 ttttgacgct tttgacgcaa gccaaataca ggttcctatt aaatcatccg gttcaccaaa
     2581 tttccccatt ggcgtgtgat taataattgt ttgacctctt ggagtaagat tatttttttc
     2641 gtctgtcaga agataacgat tttgctgcgt gaggaaaaaa ccaggtgcta ttgcattaac
     2701 ccttaaaagt ttattgtatt ccattgcaaa gtgcactgc
//
LOCUS       DAORYU010000197         2735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_24775_length_2735_cov_7.181716, whole genome shotgun sequence.
ACCESSION   DAORYU010000197 DAORYU010000000
VERSION     DAORYU010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24775_length_2735_cov_7.181716"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="PLW07_06130"
     CDS             <1..277
                     /locus_tag="PLW07_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HOL50140.1"
                     /translation="KKAGALIVGTGRSDYPNQINNLLAFPGIFRGALDARAKQITERM
                     KTEAARAIAGVIPENKLNPDYVIPSPFNKQVALAVAEAVKRVAKKSG"
     gene            280..693
                     /locus_tag="PLW07_06135"
     CDS             280..693
                     /locus_tag="PLW07_06135"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOL50141.1"
                     /translation="MVFLILCIVFYSGAALLYRHANHLHCNRLAIIVSERISAVILMS
                     LYMLFFDTFNITSGATLLAFAGGITIFISRLALLTSLKYGKISSSWTAVNLSLVIPIL
                     ASMIFWKEIPNLRQTIGLLMVPLAIILLQEEPGEK"
     gene            697..1104
                     /locus_tag="PLW07_06140"
     CDS             697..1104
                     /locus_tag="PLW07_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50142.1"
                     /translation="MWMKFILIGFFLAGISDTTWKMAGETCGASVNSYLLLFHVFALL
                     SATFAVIKTRTRIKKTEFLLGSIIGFALISGGIASMQAILRIPGIIYFPVASCGNLLI
                     VTIMANILWKEKPTNRQIAGLVIACISIVLIVL"
     gene            complement(1091..1864)
                     /locus_tag="PLW07_06145"
     CDS             complement(1091..1864)
                     /locus_tag="PLW07_06145"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOL50143.1"
                     /translation="MPEKFEQIVFVSDFDGTEQAYLIQKPASSQYRGIVIFLHGATNH
                     QQQGFNREIFNGTIGKLIDDAMEKHYIYVCPEYRGDSWMNQAAEHDLVFIIEQLEKNF
                     RTSKILLMGGSMGGTSSLIFSTRNPQLVSSCLALCPATDMKKLYYYWHGTERDFLAKT
                     IETAYGGTPEKSAAQYKERSSIYLVEKLRSTPVAIIHGDSDLVIPVEHSRRFIEKSRK
                     AGVRIYYHEIKGGNHDSPIMDFAIVKKALDWLEFAIKEQ"
     gene            complement(1840..2586)
                     /locus_tag="PLW07_06150"
     CDS             complement(1840..2586)
                     /locus_tag="PLW07_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50144.1"
                     /translation="MVKSGLWDIVLIAVVAIQAGILTYVADPRKKSLILVFPFPFTFA
                     TISLGGKIDATNVLGLVNLTLFTYGVRFFYSKLKLNIVFSIVFFALLYCFLAIVVARI
                     LPDKEIVFWISAFFVTVFALLLALLTDCPVENPYRTDLHPVIKIPVIVAVVSMLVMLK
                     KQLAGFMTVFPMVGVIAAYESRFMLASVCRQLPVLMVCLVSLMMTLKIVANITNFWVG
                     LICGWIVFLIVLKITGKFLWEELCPKNLNK"
BASE COUNT          860 a          562 c          503 g          810 t
ORIGIN      
        1 aaaaaaggca ggcgcattga ttgtgggaac aggaagaagc gattatccca atcagattaa
       61 taatcttctg gcatttcctg gaattttcag gggtgctctt gatgcaagag caaaacaaat
      121 cactgaaagg atgaaaacag aggcagcaag ggcaattgct ggagttattc ctgaaaataa
      181 actgaatcct gattatgtca taccatctcc atttaataaa caagtcgcgc ttgctgtggc
      241 agaagcagta aaaagagttg ccaaaaaatc cggataaaga tggtctttct gatactctgc
      301 attgtctttt attcaggcgc tgcgctgtta tacaggcatg caaatcatct tcattgcaat
      361 agattggcta ttatcgtttc tgaaagaatt tcagcagtca ttcttatgtc gctttatatg
      421 ttgttttttg acactttcaa catcacatct ggtgcaacat tacttgcgtt tgctggcgga
      481 attacgattt tcatttcgcg gctggcatta ctgacatcat taaaatatgg aaaaatttcc
      541 agttcctgga cagctgtaaa tctatctctc gtgataccaa tacttgcttc aatgattttc
      601 tggaaagaaa tacccaatct tcgccagaca ataggactgt tgatggtgcc tctggcaatt
      661 atactattgc aggaagaacc aggagaaaaa taagttatgt ggatgaagtt tattctgata
      721 ggtttttttc ttgctggtat ttcagatacg acatggaaaa tggcaggaga aacctgtggc
      781 gcttctgtca attcatattt actactgttt catgttttcg ccctgctcag cgccactttc
      841 gcagtcatta aaaccagaac aagaataaaa aaaactgagt ttttacttgg ctcaatcata
      901 ggatttgctc tgatttctgg tggaattgcc tcaatgcagg caatattgag aattccgggt
      961 attatttatt ttcctgttgc cagctgtgga aatttattga ttgttacgat aatggcaaac
     1021 atcctctgga aagaaaagcc aacaaatcgg cagattgcag ggcttgtaat tgcctgtatt
     1081 tctattgtgc ttattgttct ttgatggcaa actctaacca gtcaagtgcc ttttttacaa
     1141 tcgcaaagtc cattattggc gaatcgtggt tgccaccctt tatctcatga tagtaaattc
     1201 ttacacccgc ctttctggat ttttcgataa atcttcttga atgctcgaca ggaattacaa
     1261 gatcactgtc tccatgaatg attgccactg gcgttgaacg aagtttttct accagataaa
     1321 tacttgacct ttctttatac tgcgcagcgc ttttttcagg agtcccgcca taggcagttt
     1381 ctattgtttt tgcaagaaaa tccctttcag taccatgcca gtagtaataa agttttttca
     1441 tatctgtggc aggacatagc gccagacacg aggaaactag ttgcggattt cttgttgaaa
     1501 aaattaaact tgatgtacct cccatagaac cacccattaa caaaattttc gatgttctga
     1561 aatttttttc aagttgttca ataataaaca ccaaatcatg ttctgctgcc tggttcatcc
     1621 aggaatctcc cctgtattca ggacacacat agatatagtg tttttccata gcatcatcta
     1681 tcagttttcc aattgttcca ttgaaaattt ccctgttaaa tccctgttgc tgatggtttg
     1741 ttgctccatg taaaaaaatt acaatgcccc tgtattgaga actggcgggt ttttgaataa
     1801 gatatgcttg ctctgtcccg tcgaagtcag acacaaacac tatttgttca aatttttcgg
     1861 gcataattcc tcccataaaa actttcctgt gattttcaag acaatcagaa aaacaatcca
     1921 accacatatc agcccaaccc agaaatttgt tatgttggca acaatcttca atgtcatcat
     1981 caatgagaca aggcatacca tcaacacagg tagctgtctg caaacactgg caagcataaa
     2041 tcttgattca tacgcggcaa tcacacccac cattggaaaa acagtcataa aaccagccag
     2101 ttgttttttc aacatcacaa gcatcgaaac aacagcaaca atcacaggaa tttttatcac
     2161 aggatgaagg tctgttctat aaggattttc taccgggcaa tcagtcaaca aagccagcaa
     2221 gagagcaaaa actgttacaa aaaacgcaga tatccagaac acaatttcct tatcaggcaa
     2281 tatccttgca accacaatgg ctaaaaaaca ataaagcaaa gcaaaaaaca ctattgaaaa
     2341 cacaatgttc agtttcaatt ttgaataaaa aaaccgcacg ccataagtga aaagagtaag
     2401 attgacaagc cctaacacat ttgttgcatc aatttttccg ccaagagaaa ttgtggcaaa
     2461 agtaaatgga aatggaaaaa caagtatcag ggattttttc ctcgggtcag caacatatgt
     2521 caatatgcct gcctgaattg caacaaccgc aataagaaca atatcccaca gtccagattt
     2581 caccattcaa aaaataagtg gcgcaagaag tttttctgcg ttcaggtgga aaatgttgtg
     2641 agcatcttgg tctgaaacac ctgcatcaag cacagcaccg atgtaatagt gatggttaaa
     2701 ccatggccag tcagtgccaa aaacgatctg tgtcg
//
LOCUS       DAORYU010000198         2734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_24790_length_2734_cov_5.261292, whole genome shotgun sequence.
ACCESSION   DAORYU010000198 DAORYU010000000
VERSION     DAORYU010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24790_length_2734_cov_5.261292"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PLW07_06155"
     CDS             complement(<1..194)
                     /locus_tag="PLW07_06155"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOL50145.1"
                     /translation="MKAGIIGLGMGRQHLLGYLQNNIEVAGICDIDQSLLKNVQYEFH
                     IRMATADYQELLAEKDIDIIS"
     gene            complement(349..1401)
                     /locus_tag="PLW07_06160"
     CDS             complement(349..1401)
                     /locus_tag="PLW07_06160"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOL50146.1"
                     /translation="MDKIRIGIIGCGGMARGHVERFSGIKNKIVVTAVADVIRDRAEA
                     VASVLESKPIVESDYRKILKYVDAVLLVLPHHLHCEVTLECLDAGKHVLVEKPMANNE
                     EECIKMIKKQRETGLILMVGYCMRYHPLIREMKRLVDEKKYGAVFQMSIWTEQLTCYP
                     EGHWARSAKLLGGGQLFSHGCHYIDLLLWFLGKPLWGCHIGTNRGTPWMEREGTSNVT
                     IEFENGIIGYHFGTWGARGSRLKYSFHVHCEGGMLEMNREQGKLILHHRAAIHNAGIM
                     KSKQVEEVLMETPDAKPTAEEMAHFVDCILTGSTPLTDAVGSIEGLRVIWKLYEAEQK
                     HILADLRGLGLGTFKI"
     gene            complement(1423..2400)
                     /locus_tag="PLW07_06165"
     CDS             complement(1423..2400)
                     /locus_tag="PLW07_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat pathway signal protein"
                     /protein_id="HOL50147.1"
                     /translation="MNEIAGNKRQIRWGILLHLGYNMWGDRGPDYALPENVKSKYQPR
                     SYLRCEKKLWDEILKKFTRAGGNMVVIDLGEGVKYESHPELAVRGSWTPSYLKKQLAK
                     MRQMGLEPIPKLNFSTAHDNWLGKYSRMVSTDIYYKVCEDLISEVIDIFDKPRLFHLG
                     MDEETFGHQRNFLYAVVRQHELWWNDLYFLVNQVEKKDVRAWIWSDYIWEHTEDFLKK
                     MPKSVVQSNWYYGNVFNKKIMPVRAYHLLEDHGYDQIPTGSTWSYIENFTMTIEYCSK
                     HISQERLLGFLQTPWRATLRYWRKTHLAAVNEVEKGIKIFEKLRGINNS"
     gene            complement(2422..>2734)
                     /locus_tag="PLW07_06170"
     CDS             complement(2422..>2734)
                     /locus_tag="PLW07_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50148.1"
                     /translation="QGIDEYLTGLQNVFQMIKNSNHVKRFSTWLEYVKMVYESRQAEL
                     KGDFKSGIKIEERIQKFCEENQSYLKNYVTIKNVLEVSKISEQRFEEWLEKRKSSVYT
                     M"
BASE COUNT          748 a          656 c          404 g          926 t
ORIGIN      
        1 gaaataatat caatgtcttt ttctgccaga agttcttgat aatcagccgt tgccatccgt
       61 atatgaaatt cgtattgaac attttttaat aacgattggt cgatatcaca gataccagca
      121 acttcaatat tattctgaag atacccaaga aggtgctgcc tgcccattcc caatccaata
      181 atccctgcct tcatgtttca ccccgtatta acaaagttct gaataaaaat gtactcaaca
      241 tcgctttttt gaaagttcta agaagtggaa ccattctgta caaaggatac cattgattct
      301 aaaatcttat gagatggctt tattctcttt tctgtttttc atcacctttc aaattttaaa
      361 agttcctaaa ccaagccctc gtaagtcagc aagaatatgc ttctgttcgg cttcgtaaag
      421 cttccatatc actcttaatc cttctatact cccaactgca tctgtaagtg gtgtactgcc
      481 cgttaaaatg caatcaacaa agtgcgccat ttcttcagct gtgggtttgg catctggtgt
      541 ctccatcaat acttcttcaa cctgtttgct tttcattatt ccggcattat gaatagctgc
      601 cctgtggtga agtatcagct tgccctgttc tctattcatc tcaagcatgc caccttcaca
      661 gtgaacatga aaggaatact tcaatcggct cccgcgtgca ccccatgtac caaaatgata
      721 accgattatt ccattttcaa actcaatcgt gacattactt gttccttccc tttccatcca
      781 gggtgtacct cgatttgttc caatatgaca tccccagagg ggctttccaa gaaaccataa
      841 tagcaaatca atataatgac atccatgact aaagagttgt cctccaccaa gtaatttcgc
      901 agaccttgcc cagtgccctt caggataaca tgtaagttgc tctgtccata ttgacatttg
      961 aaacacggct ccatattttt tttcatcaac caatcttttc atttcacgaa ttaatggatg
     1021 gtaccgcata cagtaaccca ccattaaaat cagtcctgtt tcacgttgtt tcttgatcat
     1081 tttgatacat tcttcctcat tattagccat tggtttttca accagcacat gttttcctgc
     1141 atcaagacat tccagtgtca cttcacaatg taaatggtgt ggaagaacca gaagaactgc
     1201 atccacatat ttcaaaatct tgcgataatc actttctaca attggtttgc tttctaaaac
     1261 actggcaact gcttcagccc gatctctgat aacatcagcc acagcagtaa ctacaatctt
     1321 gttcttaata ccgctgaatc tttccacgtg tccccttgcc ataccaccgc atccaataat
     1381 acctatacgg attttatcca tattgcaaaa cctcctctta cttcaagaat tgtttatccc
     1441 acgcaatttc tcgaaaattt taattccttt ttctacttcg tttacagccg caagatgggt
     1501 ttttcgccag tatcgtaatg tggcacgcca tggtgtttgt aagaaaccaa gtaatctttc
     1561 ctgggaaata tgttttgagc aatattcgat tgtcattgta aaattttcta tgtacgacca
     1621 tgtgctacca gtgggaatct gatcataacc gtgatcttcg agtagatgat atgctctcac
     1681 cggcataatc tttttgttaa agacattgcc atagtaccag ttgctctgca ccacactttt
     1741 tggcattttc tttaagaaat cttctgtatg ttcccatata taatccgacc atatccaggc
     1801 acgaacatcc tttttttcca cttgattaac aagaaagtac aggtcattcc accatagttc
     1861 atgctgccgc accactgcat acagaaaatt tcgctgatgt ccgaatgttt cttcatccat
     1921 tccaaggtga aataatcgtg gcttatcaaa aatatctatc acttctgaaa tcaaatcttc
     1981 gcaaacctta taataaatat ccgtggaaac cattcttgag tatttcccga gccaattgtc
     2041 atgcgcagta gaaaaattga gttttggaat tggttccaat cccatttgcc tcatttttgc
     2101 cagttgtttc ttcaaatacg aaggtgtcca ggacccgcgt actgctaatt caggatgact
     2161 ctcgtatttc acaccttcac ccaggtcaat cacaaccatg ttaccacctg ctcttgtaaa
     2221 tttcttaagt atttcatccc agagcttctt ttcacacctc aaatatgaac ggggctgata
     2281 tttacttttt acattctccg gcagggcata atcagggcct ctgtctcccc acatattgta
     2341 tccgagatgt aaaagaatgc cccatcgtat ctgtcttttg ttacctgcaa tttcattcat
     2401 ccttccccct tgttaagcca gttacatcgt atacacgctg gatttccttt tttctaacca
     2461 ttcttcaaat ctttgttctg aaatttttga tacttcaaga acatttttta tggtaacata
     2521 gttttttaga taactttgat tttcctcgca aaacttctgt atcctttctt caattttgat
     2581 gccagattta aaatcacctt tcagttctgc ctgtcgactt tcataaacca tcttcacata
     2641 ttcaagccag gttgagaatc ttttaacatg attgctattt tttatcattt gaaatacatt
     2701 ttgtaaaccc gtcagatatt catcgatacc ctgt
//
LOCUS       DAORYU010000199         2729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_24853_length_2729_cov_6.459237, whole genome shotgun sequence.
ACCESSION   DAORYU010000199 DAORYU010000000
VERSION     DAORYU010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24853_length_2729_cov_6.459237"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            243..1901
                     /locus_tag="PLW07_06175"
     CDS             243..1901
                     /locus_tag="PLW07_06175"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HOL50149.1"
                     /translation="MANASTEKFQYDILEYGENKVIIIKKLIAALYSNFLKNCPLQFL
                     IIGLCCVHTGFAGIKPYEDETWNVPQNIIDTHVITCLNRYGATLRNPCSDYVFIRRVY
                     LDTIGTLPTPEEIDAFIKDTRTDKRKILIDNLLERNEFALYWTMKWCDILRVKSEYPI
                     NLWPNAVQAYHHWIYEKIRENIPYDQFVRELLTSSGSNFRVPQVNFYRAIQGRSPLNI
                     AKAVAMTFMGINPDKLPEDKQQSMASFFSRVAYKKTLEWKEEIVCLNPEGKTIRTSFL
                     DGKTVEIPPDKDPRIVFADWLISSRNSYFNKNIVNRIWAWLMGNAIISPVDDIYGKTT
                     PGQELMSCLEQELSKSNYDIKHLMRLILNSRTYQQSSIPTPQTRNPEIQFAFYRIRQL
                     EAEVLLDALCWIGGSGEEYVSQIPEPFTFIPRENRNIGLADGNITSSFLITFGRPSRD
                     TGLESERNSKPTDKQRLYMLNSSDIQKKLRTSTLLRKALSQAWKNRKECINLVYKIIL
                     SRPPTEQEMNMIEEYVANSGQSFDQAATDIAWALVNSKEFLFKH"
     gene            1958..>2729
                     /locus_tag="PLW07_06180"
     CDS             1958..>2729
                     /locus_tag="PLW07_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HOL50150.1"
                     /translation="MSILFAQGNAKSVIQIWMWGGPSQNDTFDPKVAAGYDYCGTLDK
                     TIPTNVDGIEINASLPMLAKIADKYSIIRSMTHGINGHESAAYYVQTGHKPGTGLVYP
                     SLGAIVSLFKKNEYKGKIPPYIVLTQSQGRFSEEGFLGPLYKPFVTGGDPNQKEFAVE
                     GIVAPGISDEQQKKRRELLQKMDTLGKAIPDDPDFIQFNKSEDEAYEMILGQARKVFD
                     LSQEPDEVRNKYGRNSFGQSCLVARRLVEIGVPYITINY"
BASE COUNT          925 a          514 c          544 g          746 t
ORIGIN      
        1 gtccaatgct acgagtaatc ttttttgtat ccctccagtc attatcatca acgataactg
       61 aactgctcgt aattgtatca gcgtttccat cacaaacctg acctgcaatc cagaaatacg
      121 atattaatag cgagattgcc agaaaaattt tcttatgaag catttgtacc tccctgtggt
      181 cgtaataatt ataacatccc ttgaacataa accattgttt ggtatcattg tctaactacc
      241 agatggcaaa tgcaagtaca gaaaaattcc agtatgatat acttgaatat ggtgaaaata
      301 aggtgataat aataaagaaa cttatcgcgg cattgtacag caattttctc aaaaattgtc
      361 cattacaatt tttgataatt ggattgtgtt gtgtacacac tgggtttgct ggcattaaac
      421 cgtatgaaga cgaaacctgg aatgtaccac aaaatatcat tgatacccat gttataacat
      481 gcctgaaccg atatggagca acactgcgta atccttgttc tgattatgtt tttatcagac
      541 gcgtttatct tgatacaatc ggaacgcttc ccacacccga agaaatagac gcctttataa
      601 aagatacacg aacagacaaa cgaaaaatat tgatagacaa tcttcttgaa agaaatgagt
      661 ttgccctgta ctggacaatg aaatggtgcg atatcctacg cgtcaagtca gaatatccta
      721 tcaatttatg gcccaatgct gttcaggcat atcaccactg gatttatgaa aaaataaggg
      781 aaaacattcc ttatgaccag tttgtccgcg aactgctcac atcaagcgga agcaatttca
      841 gggtaccaca ggtcaatttt tacagagcaa tacagggtcg tagcccgctc aatatagcaa
      901 aggctgtggc aatgactttc atgggaataa atcctgacaa actaccagaa gataagcagc
      961 aaagcatggc atcgtttttt tcccgggttg catacaagaa aacacttgaa tggaaggaag
     1021 aaattgtttg tcttaatcca gaaggaaaaa ccatcagaac aagttttctt gacgggaaaa
     1081 cagtggaaat tccgcctgat aaggacccga gaattgtatt tgctgactgg ctcatttctt
     1141 cacgaaattc atattttaat aaaaacatcg tcaatagaat atgggcatgg ttaatgggaa
     1201 atgccattat ttctcctgtt gacgacatct atggaaaaac cacacctggc caggagctta
     1261 tgtcctgtct ggaacaagaa ctgagtaaat ctaactatga catcaaacat ttgatgaggt
     1321 tgattcttaa ttctcggaca tatcaacaat cttcaatacc taccccgcaa acaagaaatc
     1381 ctgaaataca gtttgcattt tacagaatca ggcagcttga agcagaggtc cttctggatg
     1441 cattgtgctg gatcggtggt agtggagaag aatatgtgag tcagattcca gaaccattta
     1501 cctttattcc aagggaaaat agaaatatcg ggcttgcaga tggcaatatc accagttctt
     1561 ttcttatcac atttggcaga ccatcaagag acacaggtct tgaatcagaa cgtaacagta
     1621 aaccaacgga taaacaacgt ctgtatatgt tgaattcaag cgacatacag aaaaaattga
     1681 gaacaagtac tcttctaaga aaagcgttat ctcaggcatg gaaaaacagg aaagagtgca
     1741 ttaaccttgt ttataaaatc atattgtcaa gaccgccaac agagcaggaa atgaacatga
     1801 ttgaagaata tgtagcaaat tcaggacaat cgtttgatca ggcagcgaca gatattgcct
     1861 gggcattggt aaacagtaag gaatttttgt ttaaacatta aaggaggcaa taaacgtgaa
     1921 tcgttttctt ttgtcttgta caattataat cctatcagtg agcattcttt ttgcacaggg
     1981 taacgcaaaa tctgtcattc aaatatggat gtggggaggt ccatctcaga atgatacctt
     2041 tgacccgaaa gttgctgctg gctatgatta ttgcggcaca cttgataaaa caataccaac
     2101 aaatgttgat ggcattgaaa tcaatgcgtc tttaccaatg cttgcaaaaa ttgcagataa
     2161 atattcaatt atcaggagca tgacgcatgg catcaatgga catgaatcag cagcatatta
     2221 cgttcaaaca ggccacaaac caggaacagg gcttgtatat ccttctcttg gtgcgatagt
     2281 cagtttattc aagaaaaatg aatacaaagg taaaattcct ccatatattg tactgacaca
     2341 atctcagggc aggttctcag aagaagggtt tcttggacca ttgtataagc catttgttac
     2401 aggcggagac ccgaatcaaa aggaatttgc tgtggaagga attgtggctc caggtatatc
     2461 tgacgagcaa cagaaaaaac gaagggaatt gcttcaaaaa atggataccc tgggcaaagc
     2521 aattccagat gaccctgatt ttattcaatt taacaaaagt gaagacgaag catacgaaat
     2581 gattcttgga caggcaagaa aggtgtttga cctttctcag gaacccgatg aagtaaggaa
     2641 taaatatggc agaaactcgt ttggtcagag ttgtcttgtt gcaagacgac ttgttgaaat
     2701 tggtgtgcca tatatcacaa tcaattacc
//
LOCUS       DAORYU010000200         2727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_24875_length_2727_cov_6.423653, whole genome shotgun sequence.
ACCESSION   DAORYU010000200 DAORYU010000000
VERSION     DAORYU010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24875_length_2727_cov_6.423653"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="PLW07_06185"
     CDS             <1..456
                     /locus_tag="PLW07_06185"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50151.1"
                     /translation="KNVKFAIDEYGFGKHFRWYMPDFDRSMWRLIDTTKPFYIQGYLS
                     PDGMPYMGKMWYIFDLDIPKGVAGKQIKLYAPFVTCEAWVWVNGQYAGHRKYLEAYIS
                     PAEIDIDITPFTRPGQKNTIAVWVSTGLSPAQAADGFLGRLFLYSPISR"
     gene            507..1553
                     /locus_tag="PLW07_06190"
     CDS             507..1553
                     /locus_tag="PLW07_06190"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOL50152.1"
                     /translation="MPIRLWGVDPMFPVRDDWKPLIELTEKHQIEIIRTWAPSGEEIP
                     EPVCKQYDTEKTIPGKEIKEIITTIETPAGLLTQIFYQPLSGAPGYIKKHFIGDTTDA
                     KKWLSIPDSRLPKIDSYFELEKKTGSRAMLMIGIDEAMYMVQRLMGSEVFGYWLYDER
                     ELLKEMIDRAYRKIEKLVKHYLSSKLGDAYGWVGPELCIPPLASVKDFYEFVVEYDRK
                     IIDLIHESGKLSWIHCHGDMKPVLEGFIDMDLDCLNPIEPPPVGKITLAEAKHICKGK
                     MCLDGGIEDGIFYTCKPEQIRQITIETIQQGKPGGGFILCPSSSPNTAAVLPEYVIEN
                     YKIFVETAVELRNY"
     gene            1703..2188
                     /locus_tag="PLW07_06195"
     CDS             1703..2188
                     /locus_tag="PLW07_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019691058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HOL50153.1"
                     /translation="MQIITVKIEKPESINVILGQTHFIKSVEDIHEGLVSAVPGIKFG
                     MAFCEASGKCLVRWSGTDSAMIELAKSNAKNIAAGHSFIIFLGENFYPVNVLNTIKMV
                     PEVCRIFCATANPVEVVIAETEQGRGILGVIDGFSTKGIEDEEEIKWRKDLLRKIGYK
                     F"
     gene            2250..>2727
                     /locus_tag="PLW07_06200"
     CDS             2250..>2727
                     /locus_tag="PLW07_06200"
                     /inference="COORDINATES: protein motif:HMM:NF012868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HOL50154.1"
                     /translation="MEPSFFALGTIFVPVLGCITVPLADLVSARFRNLWALILGIATF
                     VLPLFLIPAVLQSPVGEIIYSYPSILNLNFILLIDGLSVFVALVSSFIGALIILYSFD
                     YVSHNDFQTEYYTMVVLFIGSMMGLVFSGNLVFMYLFWEIAAICSWRLIGFYRGPSH"
BASE COUNT          824 a          428 c          610 g          865 t
ORIGIN      
        1 aaaaatgtta aatttgcaat tgacgagtac ggttttggta agcatttcag gtggtacatg
       61 cctgattttg atagaagcat gtggcgattg attgacacga caaaaccttt ttatatacaa
      121 gggtatcttt ctccagatgg tatgccgtat atgggaaaaa tgtggtatat ttttgacctt
      181 gatattccaa aaggtgttgc tgggaaacaa ataaaattgt atgcaccctt tgtaacatgt
      241 gaagcatggg tgtgggtcaa tggtcagtat gcaggacata gaaagtacct tgaggcatac
      301 atcagtcctg ctgaaattga tatcgacatt acgccattta ccagaccagg gcagaaaaat
      361 actattgctg tgtgggtcag tacaggattg agtcctgcgc aggcagcaga tggttttctt
      421 ggaagattgt tcctttattc acctataagc cgatgacacc aagggaaagg ctcattagag
      481 tttttcaatg tcttgatgtt gacaggatgc ctatcagatt gtggggtgtt gaccctatgt
      541 ttcctgtaag ggatgactgg aaaccattaa ttgaacttac agaaaaacac cagattgaaa
      601 ttattagaac ctgggcccct tcaggtgaag aaattccaga acctgtttgt aaacaatatg
      661 atactgaaaa aacaattcct ggaaaagaaa taaaggaaat aatcacgacc attgaaacac
      721 ctgctggttt gctgacccag attttttatc agccactttc aggtgctcct ggatatatta
      781 aaaaacactt cattggggat acaactgatg caaaaaagtg gctttcaatt ccagatagca
      841 gactgccgaa aattgattct tattttgaac tggagaaaaa aactggctca agagcaatgc
      901 tgatgattgg tatagatgag gcgatgtata tggttcaaag gttgatgggt tcagaggttt
      961 tcggttactg gctttatgat gaaagggaac ttttgaaaga aatgatagat agggcataca
     1021 ggaaaataga aaaacttgta aaacattatc tatcatcaaa acttggtgat gcatatggat
     1081 gggttggacc tgaactttgt attcctcctc ttgcatcagt gaaagatttt tatgaatttg
     1141 ttgtagaata tgatagaaaa atcattgacc tgattcacga gtcaggaaag ctaagctgga
     1201 ttcattgcca tggagatatg aaacctgtat tagagggatt tattgatatg gatcttgatt
     1261 gtttgaatcc aattgagcca ccaccagttg gtaaaattac tcttgccgaa gcaaaacata
     1321 tttgcaaagg gaaaatgtgt cttgatggtg gcatagaaga tggaattttt tatacctgca
     1381 aaccagaaca aataaggcag attacaatag aaacgattca acaaggaaag ccaggaggtg
     1441 gctttatact ctgtccatca tcttctccga atactgcagc agtgttgcct gaatatgtga
     1501 tagaaaatta caaaattttt gtagaaactg ctgttgaatt gcggaattat tgagttacag
     1561 ttatgccagg ttactaatga atgttgtttc attgattttt tctctcgtga tggaagaagg
     1621 tgggactgag agtagaaaga ggaaaaattg tccatttgtg caaagtttct gacagaacca
     1681 gattttttaa aaggggcttg atatgcaaat catcactgta aagattgaaa agccagaatc
     1741 catcaatgta attcttggac agacacattt tattaagtca gttgaagata ttcatgaagg
     1801 gcttgtttcg gcagtgcctg ggatcaaatt tggtatggcg ttttgtgaag cttcaggcaa
     1861 atgtcttgtg cgatggtctg gaacagattc tgcaatgatt gaacttgcaa agtcaaatgc
     1921 aaaaaacatt gctgcaggcc acagttttat catttttctt ggtgagaatt tttacccagt
     1981 caatgtgttg aatacaataa aaatggtgcc agaggtatgc agaatttttt gtgcgacagc
     2041 aaatccagtg gaagtggtaa ttgctgaaac agaacaggga agaggcattc ttggtgtaat
     2101 cgacggattt tcaacaaaag gcatagagga tgaggaagag ataaaatggc ggaaggattt
     2161 actaagaaaa attggatata aattttaatt ttaagatttt tgatgatttt ttgacagcat
     2221 cataattttt gattaaacta gtaggccgta tggaaccatc tttttttgct ctcgggacga
     2281 tttttgtgcc agtgcttggc tgtatcacgg taccactggc tgacctggtt tctgccagat
     2341 tcagaaattt gtgggctctg attcttggta ttgccacgtt tgtcttgccc ctttttttga
     2401 ttccagcagt actccagagt cctgttggtg aaataattta tagttatccg tccattttga
     2461 atctcaactt tatacttctc atagatgggc tgtcggtttt tgtcgctctt gtttcttctt
     2521 ttatcggcgc cttaataatt ttatactcat ttgactatgt gtctcacaac gattttcaaa
     2581 cagaatatta cacaatggtg gtactgttta ttggctcaat gatgggactt gttttttccg
     2641 gaaatttggt ttttatgtac cttttctggg aaattgcagc aatatgttca tggagattga
     2701 tcggtttcta tcgtggacca tctcata
//
LOCUS       DAORYU010000201         2722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_24938_length_2722_cov_5.105737, whole genome shotgun sequence.
ACCESSION   DAORYU010000201 DAORYU010000000
VERSION     DAORYU010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24938_length_2722_cov_5.105737"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..1044
                     /locus_tag="PLW07_06205"
     CDS             76..1044
                     /locus_tag="PLW07_06205"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOL50155.1"
                     /translation="MLKRKLGNSGMEITIVGLGTWAIGGTGYNYSWGSQDDKDSIETI
                     KAAINLGINWVDTAPAYGLGHSEKIVGIATKDHRDNVFIFTKLGKSWDEKGLLGDGLS
                     GKTVRQETLQSMERLGVDQIDLMQIHWPRPDTEIESAWEQMCKLVDEGKIRAIGVSNF
                     NVEQLERIKKIRVPGSLQPPYCIFRREIEKEILPWCKEHNVGVICYSPMYYGLLAEKF
                     DEQRISKMSPDDWRRKLPELTTNHKTYCEFYDRFKEIADECKMTTGQLAIAWVLAHPA
                     VTGAIVGARKPQQIKEIVSNCTFEISASIKEKIDSLSEQYSPLIRR"
     gene            1048..>2722
                     /locus_tag="PLW07_06210"
     CDS             1048..>2722
                     /locus_tag="PLW07_06210"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOL50156.1"
                     /translation="MKHEFSFDVFRKSPVEYRPAPFLFLNHKMEDDELIWQVQQIKDK
                     GLRGFFMHPRPGLLTPYMSVEFREKITLMVEKAAELGIEAWLYDEDPYPSGAAGGKVF
                     YQYPEFMSRTLVPVVATTAGKREFKLDMPIGYVVRVFAIRLDENARADTEKVVDITEY
                     VGPIRDQWGYVRRYNTYYGSMRADTFPHWRSDATGIKNRLLWNVPDGKWKIIGFIETF
                     DKSYWGPWGGYVDLLNKDAVDDFIKKTHEVYKNDVGKYFGNTIPGIFTDEPKWIGWLP
                     WSSKLPEFFRKAKGYDILPHLYLLLGGDTTQARTIRFDYWDVLTRMLKHAFFDNISRW
                     CENNNISFTGHVSPEEEPDLGVVYLGDLMQHAKAFQIPGTDIITSRLGTNEFPVVSVG
                     PKLISSVARQMGRERVLSECFALEEWDFTLEKMKKIADFMMALGVNFINPHGFYYSID
                     GHRKKEANPSQFYQTTYWQYYEQFSRYIGRVCYMLTRFDYPVEFALLYPTSSLWQLLP
                     ADREKAKELSDTFVFINNVFVHACRQFDFVDDIDFANAKIENSAFKIGKM"
BASE COUNT          869 a          446 c          651 g          756 t
ORIGIN      
        1 ccttattcca ggggaaggta ttgaaatcaa gccagagtgg ataaaaggaa cacggcttga
       61 agatggaggt ggaacatgct gaaaagaaaa cttggtaatt caggtatgga aattacaatc
      121 gttggacttg gtacatgggc gattggtgga actggctata attattcttg gggctctcag
      181 gatgataagg attccataga aacgataaag gccgcaatta atcttggtat taactgggtt
      241 gatacagcgc ctgcctatgg actggggcac tctgaaaaaa ttgttgggat tgcaacaaag
      301 gatcatagag acaatgtatt catttttaca aaacttggaa aaagctggga tgaaaaaggc
      361 ttgcttggag atggactgtc aggaaaaaca gtgagacagg aaacactgca aagtatggaa
      421 cgacttggag tggaccaaat tgatttgatg caaatacact ggccaagacc tgatacagaa
      481 attgagtctg cctgggaaca gatgtgtaaa cttgtggatg aaggaaaaat tagagccatt
      541 ggagtatcga attttaatgt tgagcagctt gaaagaataa aaaaaataag agttccagga
      601 tcgttgcagc cgccatattg tatttttaga agagaaattg aaaaggaaat actgccttgg
      661 tgtaaggaac ataatgtggg agtcatctgt tatagtccaa tgtattatgg gcttcttgca
      721 gaaaagtttg atgagcaaag aatttcaaaa atgtctccag acgattggag aagaaaattg
      781 ccagaattaa ccacgaacca caagacatac tgtgaatttt acgacaggtt taaggaaatt
      841 gcagatgagt gcaaaatgac aacaggacaa ctggcaattg cgtgggttct tgcgcatcca
      901 gcagtgactg gtgctattgt tggcgcaaga aagccacaac agattaaaga aattgtcagc
      961 aattgcacgt ttgagatttc tgccagcatt aaagaaaaaa ttgactcatt atcagaacaa
     1021 tactcacccc ttataagaag gtgaaacatg aaacatgagt tttcttttga tgttttcaga
     1081 aagtcgcctg ttgaatacag gccagcgcca tttttattct tgaaccacaa aatggaagat
     1141 gatgaactga tatggcaggt gcaacaaatc aaggataaag ggctaagggg attttttatg
     1201 catcccagac caggactgct cactccttat atgtcagttg agttcaggga aaaaatcaca
     1261 ttgatggtgg aaaaggcagc agaattgggt atagaggcat ggttgtatga cgaagacccg
     1321 tatcccagtg gtgctgctgg tggaaaggtt ttttatcaat atcctgaatt tatgtcaagg
     1381 acattagtac ctgttgttgc aaccactgct ggaaaacgag aattcaaact ggatatgcct
     1441 atcggatatg ttgtcagggt gtttgcaatt cggcttgatg aaaatgcgcg cgctgatact
     1501 gagaaggttg ttgacattac agagtatgta ggtccaatac gggatcaatg ggggtatgtg
     1561 agaagataca atacctatta tggttcaatg cgggcagata cctttcctca ctggaggtca
     1621 gatgccactg gaataaaaaa ccgtcttttg tggaatgtcc ctgacggaaa atggaagatt
     1681 atcgggttta ttgaaacatt tgataaaagt tactggggtc catggggtgg ttatgttgac
     1741 ctgcttaata aggacgctgt tgatgatttt atcaaaaaga cccacgaagt gtataaaaat
     1801 gatgtgggca aatattttgg aaatacaatt ccaggcattt ttacagatga accaaagtgg
     1861 ataggatggt tgccctggtc cagcaaactt cctgaatttt tcagaaaggc aaaaggatat
     1921 gatattttgc cacatcttta tttgcttctt ggtggtgata ccacacaggc aagaacaatc
     1981 aggtttgact attgggatgt tcttacaaga atgttgaagc atgcattttt tgataacatt
     2041 tcacgctggt gcgaaaacaa taacatttcc ttcacaggcc atgtcagccc tgaagaagag
     2101 ccagaccttg gagttgtgta tctcggcgat ttgatgcagc atgcaaaggc attccagata
     2161 ccaggcacag acattattac ttcaagattg ggaacaaatg aatttcctgt tgtaagtgtt
     2221 ggtcccaagc tcatcagttc tgttgcaagg caaatgggaa gagagcgtgt gctttcagaa
     2281 tgctttgcgc ttgaagagtg ggattttacg cttgagaaga tgaaaaaaat tgctgacttt
     2341 atgatggcgc ttggagttaa tttcatcaac ccgcatggtt tttattactc catagatggt
     2401 cacagaaaaa aggaagcaaa tccttctcaa ttctaccaaa caacatactg gcagtattat
     2461 gaacagttca gtagatacat tggaagggtc tgctatatgc tgacaaggtt tgattaccct
     2521 gtcgagtttg ctcttttata tccaacttct tcattgtggc aactgcttcc tgctgataga
     2581 gaaaaagcaa aagaattaag tgatacattt gtctttatca acaatgtttt cgttcacgct
     2641 tgcagacagt ttgattttgt tgatgacatt gactttgcaa atgcaaagat tgaaaacagt
     2701 gccttcaaga tcggtaaaat ga
//
LOCUS       DAORYU010000202         2682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_25470_length_2682_cov_4.542063, whole genome shotgun sequence.
ACCESSION   DAORYU010000202 DAORYU010000000
VERSION     DAORYU010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25470_length_2682_cov_4.542063"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /gene="guaA"
                     /locus_tag="PLW07_06215"
     CDS             complement(<1..743)
                     /gene="guaA"
                     /locus_tag="PLW07_06215"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOL50157.1"
                     /translation="MVVILDFGSQYTQLIARRIRESGVYCEIYPYHVSASTLKGKNID
                     GLILSGGPGHLTESEISFLPDPEIFKANYKILGICFGMQVIAKFSGAKVSKGAVREYG
                     KTIFYPRKDVIFENVPEKTIVWMSHYDQVENLPEGFHLIGSTANCKIAAFRNKEGSIY
                     GLQFHPEVVHTQHGKTILKNFLYKIAGCRSDWTPSSMVETAKREITSKIGGSKVVCAL
                     SGGVDSSTLAVLLHQVAGERSLAVFVNHGL"
     gene            complement(727..2133)
                     /gene="purF"
                     /locus_tag="PLW07_06220"
     CDS             complement(727..2133)
                     /gene="purF"
                     /locus_tag="PLW07_06220"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006420443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOL50158.1"
                     /translation="MEFIKENCGIAGVFRHKDASEILYLSLFSLQHRGQESAGILLTD
                     GSSVFFHRAQGLVSEIFTPEIIHSLKGNCGIGHVRYSTTGASNEKNIQPFSVEYKGKT
                     LAVAHNGNLTNSRILRTKLEKQGSIFQTSMDSEIILHLLVRSKGSTIKEKLAHALRQL
                     KGAFSILIFTENEIIAARDANGFRPLCIGKLDSSYVVASETCAFDLIGAQYFRDVEPG
                     EMVIFSDNGIDSYQWAEKTKKAHCIFEFIYFARPDSYIFTKSVYLTRKNLGVNLAREC
                     DFDGDLVMPVPDSGSIAALGFAQEKKIPIEFGVIRNHYVGRTFIQPAQKIRDAGVRIK
                     LNPVSAVVKGKDVIVVEDSIVRGTTCRNRINSLRTAGAKRIMLVISCPPIISPCYFGI
                     DFPSTQELIASTKSIKQIKDFLGLDGLHYLSYKGMLDAMLIPGENFCTGCFTGKYPVK
                     PDTMFNKYQWEKSHGCDS"
     gene            complement(2354..>2682)
                     /locus_tag="PLW07_06225"
     CDS             complement(2354..>2682)
                     /locus_tag="PLW07_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50159.1"
                     /translation="MVLAICWLCAISFSKEKFSASEEPAKNIGEVTKVDTSLFCFTIR
                     DQEGTETKFFTSPMKIRMINPGENIQVGYKKTTDGQLKALNIKQVKEKKKGKKTEKQG
                     KKHSSP"
BASE COUNT          787 a          579 c          457 g          859 t
ORIGIN      
        1 agaccatgat taacaaagac agcaagcgac ctttctcctg ccacctggtg aagaagcact
       61 gcgagtgttg aagaatcaac tccaccgctt aatgcgcaaa cgaccttact gcctccaatt
      121 tttgatgtga tttctctttt tgctgtctca accattgaag aaggagtcca gtcagacctg
      181 catccagcaa ttttatatag aaagtttttc aaaatggttt tgccatgctg ggtatgaacg
      241 acttctgggt ggaattgaag cccgtatatt gaaccttctt tatttctgaa tgctgcgatt
      301 ttacaatttg ctgtgctgcc aattagatga aatccctccg gtaaattttc aacctgatca
      361 taatgactca tccacacaat cgttttttct gggacatttt caaaaattac atccttcctt
      421 ggataaaaaa ttgtttttcc gtattctctc acagcacctt ttgaaacctt tgcgccagaa
      481 aactttgcaa taacctgcat accaaagcag ataccaagaa ttttatagtt cgctttaaaa
      541 atttcagggt caggcagaaa agaaatctct gactcagtca ggtgtcctgg tccaccagaa
      601 agaatcaaac catcaatgtt ttttcctttc agagtagaag cagacacatg atatggatat
      661 atttcacaat aaacccctga ttcccttatc cttcttgcaa tcaactgcgt atattgagaa
      721 ccaaaatcaa gaatcacaac catgtgattt ctcccactgg tatttattaa acattgtatc
      781 aggttttaca ggatactttc cagtaaaaca accggtgcaa aaattttcgc caggaataag
      841 catcgcatca agcattcctt tataactcaa ataatgaagc ccatcaagcc cgaggaaatc
      901 tttgatttgc tttatactct ttgttgaagc aatcaattcc tgtgttgaag gaaaatcaat
      961 gccaaaataa cacggagata taattggcgg gcaggaaatc acaagcataa ttctttttgc
     1021 gccagcggtt cttaatgagt ttattctgtt tctgcatgtg gtaccccgga caattgagtc
     1081 ctctacaaca atcacatctt ttcctttcac aactgcgctg acaggattta attttattct
     1141 gacgcctgca tctctgattt tctgtgctgg ctgaataaat gtccttccga cataatgatt
     1201 cctgattaca ccgaactcaa tcggaatctt tttttcctga gcaaatccga gcgcagcaat
     1261 agaaccagaa tcaggcacag gcatcaccag gtctccgtca aaatcacatt cccttgcaag
     1321 atttacgcca agatttttcc ttgtaagata cacgcttttt gtaaagatat aactgtctgg
     1381 tcttgcaaaa taaataaatt caaatatgca gtgcgccttt tttgtctttt cagcccactg
     1441 ataactgtca atcccattat ctgaaaaaat caccatttca cctggttcaa catctctgaa
     1501 atattgcgcg ccaattaagt caaatgcgca tgtttctgaa gcaacaacat aactactatc
     1561 aagtttacca atgcaaagtg gcctgaaacc atttgcatcc cttgctgcaa tgatttcgtt
     1621 ttcagtaaaa atcaggatgg aaaaagcgcc tttgagttgc ctcaaagcgt gtgccagttt
     1681 ttccttaatt gttgaacctt tgcttctcac aagaagatgc aaaataatct cactatccat
     1741 gcttgtttga aaaatagaac cctgtttttc aagttttgtt ctcaaaattc tgctgtttgt
     1801 gagattgcca ttgtgggcaa ctgctaatgt ttttccttta tattcaacag aaaatggctg
     1861 gatgtttttt tcgttgcttg cgcctgtggt tgagtatctc acatgaccta tgccgcaatt
     1921 acctttgagt gaatggatga tttcaggagt aaaaatttca gaaacaagac cctgcgccct
     1981 gtggaaaaaa accgatgacc catcagtcaa caatattcct gcgctttctt ggcctctgtg
     2041 ctgaagagaa aacaaagaaa gataaagaat ctctgaggca tctttatgcc tgaaaacacc
     2101 agcaatacca cagttttctt ttataaattc cattgtatgt tcctttaaga aagtattcat
     2161 ccggctggac aaattatatt accacttttt ccagaatttc tcaaactgaa gccgacatat
     2221 tttattgaaa tcataggaga aagaatcctg ctttgaattt aaggctttga gtttgaaggc
     2281 ctttttttca ccctcatccc ctaccctgtc caatcaagaa agagggattc agaagtgggc
     2341 ttgcagaaga ggattaaggt gaggagtgct tttttccctg tttttctgtt ttctttccct
     2401 tctttttctc tttaacttgc ttaatgttca atgcctttaa ttgaccgtca gttgtttttt
     2461 tatagccaac ttgaatattt tcaccaggat taatcatcct gattttcatt ggagaagtaa
     2521 aaaactttgt ttctgtacct tcttggtctc ttatcgtaaa acaaaaaagg gacgtatcga
     2581 cctttgttac ctcaccaata ttttttgcag gttcttctga cgcagagaat ttttcttttg
     2641 aaaaagaaat tgcacaaagc cagcagatcg caagcaccat ag
//
LOCUS       DAORYU010000203         2676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_25547_length_2676_cov_3.669592, whole genome shotgun sequence.
ACCESSION   DAORYU010000203 DAORYU010000000
VERSION     DAORYU010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25547_length_2676_cov_3.669592"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="PLW07_06230"
     CDS             <1..745
                     /locus_tag="PLW07_06230"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOL50160.1"
                     /translation="ATLAKGTTVIEFAACEPEIVDLANFLVKMGAKITGIGSPCLTVY
                     GVKKLKPVEHKIIPDRIETGTFLLAGAITKGCVTVEKCQPLHLGAFLERLGETGVSIK
                     NGKNFITVSGISGWKPVDIVTLAYPGFPTDLQAQMMAFLSLADGVSVITEKVFPERFI
                     HAGELNRLGANIQLDGSRAIVRGVKKLLGARVMASDLRASAAMVLAGLSAEGTTEVSR
                     IYHLFRGYHRFEEKLMCLGARITRQKDAS"
     gene            742..1545
                     /gene="dapB"
                     /locus_tag="PLW07_06235"
     CDS             742..1545
                     /gene="dapB"
                     /locus_tag="PLW07_06235"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013100126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOL50161.1"
                     /translation="MTEQKLKIAVAGVCGRMGSTIVSLLKNRADMKLAWAFESKHHPS
                     VGTEVLPGVNIREDFQQCQQADVLIDFTIPEATITNVKIAQQKNVKVVIGTTGFSSEQ
                     LDIIKSASEKTAIVMSPNMSIGVNIMMKLVQIASEMLGPDYEVEIIETHHHNKKDAPS
                     GTALKLGEIINKTRNKDARSDFVCGRNGIVGPRKSEEIGIHAVRISEIVGEHTVMFGG
                     KGEILEVSHRCFSRESFASGALIAAKFVAGKERGLYEMKDVIEWLRLNP"
     gene            2046..>2676
                     /locus_tag="PLW07_06240"
     CDS             2046..>2676
                     /locus_tag="PLW07_06240"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOL50162.1"
                     /translation="MFEVSEKMKKLPPYLFAEIDKKRKQLIAEGHQVISLGVGDPDLP
                     TPERIVNAMKKAVEDPGVHRYPFGKGRADFRRAIADYYKKHSDVDLDPDNEICVLIGS
                     KEGIAHFPFTCINPGDITLVPEPGYPVYQIGTILAGGTCYFMPLKEENNFLPDLGKIP
                     SEIAEKAKILYLNYPNNPTAACADIDFLKDVIAFCKKHQIIILYDAAYHE"
BASE COUNT          834 a          463 c          604 g          775 t
ORIGIN      
        1 ggcgacatta gcaaaaggaa caacagtgat tgagtttgca gcgtgtgagc cagaaatagt
       61 ggaccttgca aactttctgg tgaaaatggg tgcaaaaatt acaggtattg gttcaccctg
      121 tttgactgta tatggagtca aaaaactgaa acctgtggag cacaaaatta tccctgacag
      181 gattgaaaca gggacctttc ttcttgcagg tgcaattaca aaaggatgtg tgacggttga
      241 aaaatgtcag ccattgcatc tcggcgcgtt tcttgaacga ctgggtgaaa caggagtttc
      301 aattaaaaac ggtaaaaatt ttattactgt cagtggtatt tcaggatgga aacctgtgga
      361 cattgttacc cttgcgtatc cagggtttcc aactgacctg caggcgcaga tgatggcatt
      421 tttatctctt gcagacggag tgagcgtaat tactgaaaag gttttccctg aaagatttat
      481 acatgcgggt gaactcaacc ggcttggagc aaatattcaa ctggatggtt caagggccat
      541 tgtcaggggt gtgaaaaaac tccttggagc aagggttatg gcttcagatc tcagagccag
      601 tgctgccatg gtacttgcag ggttatctgc tgaagggact acagaggttt caaggattta
      661 tcaccttttc aggggatacc acagatttga agaaaaactt atgtgtctgg gcgcaagaat
      721 aacacgacaa aaggatgcat catgactgaa caaaaactca aaattgctgt ggcaggtgtt
      781 tgtggaagaa tgggctcaac aattgtatcg ctgttgaaaa atcgtgctga tatgaaactg
      841 gcatgggcat ttgaatcaaa acatcatcca tctgttggaa ctgaggtact accgggagtc
      901 aatatcaggg aagattttca gcaatgtcaa caagcagatg ttttgattga ctttacaatt
      961 ccagaagcaa caattaccaa tgtgaaaatt gcgcagcaaa aaaatgtgaa agttgtcatt
     1021 ggaactactg gtttttcttc agagcaactg gatattataa aatctgcgtc agaaaagaca
     1081 gcgattgtga tgtcaccgaa catgagtatt ggagttaata ttatgatgaa actggttcag
     1141 attgctagtg aaatgctggg accagattat gaggttgaga taatagaaac ccatcatcac
     1201 aataaaaaag atgctccaag cggtactgca ttgaaacttg gtgagataat aaataaaacg
     1261 cgcaataaag atgcaaggtc agattttgtt tgtggaagaa atggtattgt gggtccaaga
     1321 aaatcagaag aaattggaat tcatgctgtg agaatttcag aaattgtagg agaacatact
     1381 gtaatgtttg ggggtaaagg agaaattctt gaagtgagcc acaggtgttt cagcagggag
     1441 tcattcgcat caggcgcgct gattgcagca aagtttgttg ctggcaaaga acgcggtttg
     1501 tatgaaatga aagatgtcat agaatggctc aggttaaatc cctgaaatag attcaaaata
     1561 aattttatat tttctcaaca tccattgttt gtaaaatgtt tttttctttt tcccttttac
     1621 agaaaaattt cattctccct ttcacaaaat ttcttttccc atccgtcccc ttataaaatt
     1681 gctcttccat tcttctcctt tgcaaaattt ctcttcccat cctccccctt tacaaaaggg
     1741 ggattaaggg ggatttttga aatttccccc aacccctttt ttataaagag gggtgaggaa
     1801 tgaggattga taaaggaagg tgtcctgaag tcagagcaaa gtttataatg aaacgtaaga
     1861 cgattcattt gtagaatcag tttcgtactt acctgtttag catttcataa gaaattgtgc
     1921 taatatattt ccagccgggt tttttgcatg atactacatc taagtaattt cgccctttca
     1981 gggaccggct ccagaatagg attattccaa aaattttatt gaacaataat gattcggagg
     2041 agaatatgtt tgaagtaagt gaaaaaatga aaaaactgcc gccgtatttg tttgctgaaa
     2101 ttgataaaaa acgaaaacag ttaattgcag aagggcacca ggttatcagt ttaggagttg
     2161 gagaccctga ccttccaacc cctgaaagga ttgtcaatgc gatgaaaaag gcagttgaag
     2221 atcccggtgt tcacagatat ccttttggaa aaggcagggc tgattttcgc agggcaatag
     2281 cggattatta caaaaaacac tcagatgttg accttgaccc tgataatgag atttgtgtgt
     2341 tgattggttc aaaggaaggc attgcacatt ttccgtttac ctgtatcaac cctggtgata
     2401 ttacccttgt tcctgagcca ggatatccag tttaccagat agggacaatc cttgctggtg
     2461 gcacatgtta ttttatgcca ttgaaagagg aaaacaattt tttgccggat ctcggtaaaa
     2521 ttccgtcaga aattgcagaa aaggcaaaga ttctttacct gaattatcca aacaatccga
     2581 ctgctgcctg tgctgatatt gactttttga aagatgttat tgcgttttgc aagaaacatc
     2641 agattatcat tctttatgac gctgcgtacc acgaga
//
LOCUS       DAORYU010000204         2668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_25636_length_2668_cov_4.611558, whole genome shotgun sequence.
ACCESSION   DAORYU010000204 DAORYU010000000
VERSION     DAORYU010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25636_length_2668_cov_4.611558"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="PLW07_06245"
     CDS             <1..955
                     /locus_tag="PLW07_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50163.1"
                     /translation="VSSICSGFALSSVLSYGYVFFTVILSIFFVANGVRGILKNVAIA
                     FICFIFSYFLLNPYVLLSFNEFVNELSYIQSYWRNSVTLENLKYFLTMSLRYGLGTGV
                     WLVSVIFLIFSLYFSRDKQFLVILLSLLPAVLYFGIITGRWVHYSFVIYPYIFLIILF
                     GATKIGVKGSMLILLPVLIFTFIFSFAHVTLFCGKNIRSVAGDWVNKNIPENSSMGLL
                     EAPSPWRTPPFQFLKYDIRIGTPEQIQKPEYFIVSQYQWLRGGSFKDIKNLLADYEII
                     KEFRKEPVFLGLKFRQSDNIPYDWCHPNPIILIWKRKIQKF"
     gene            928..2142
                     /locus_tag="PLW07_06250"
     CDS             928..2142
                     /locus_tag="PLW07_06250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOL50164.1"
                     /translation="MEKKNSKILILIFFVAFVARICFILTLDNSVDVWGDWWDELGWK
                     LASGQGYWVNNPYFVDGEPFYSWRAPGFPLFLAGIYKIFGHSFLAAKIGLAFISSLTA
                     VFLFPLAAFFVGNRAAMACAAFYAVYPVAIFWTGYLAPETLTAFFVVVLTFLSVLAVR
                     TDRLLICFVSGIIFGCLFLTRPLTIVLFPVILVYFLIHKQKIVKKSLLFCTGCLIVFP
                     WIVRNYLIHHTLVLGSTEGGVVFYIANNEKVLGEPSGFYHAENITEFRGLSEVEIDRK
                     FYRMGFDFVKKNPSLYFKLVMDRFVRFWRFFPHTISGPGEPYSKKHVIISVFVETPLI
                     ILGWIGFFLSFRQWRNFFVLHGFIVTFSTFTILIRTAIRYRFPVMPLMLVFAIYTLWL
                     FKKFRENHARKF"
     gene            2126..>2668
                     /locus_tag="PLW07_06255"
     CDS             2126..>2668
                     /locus_tag="PLW07_06255"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOL50165.1"
                     /translation="MPGNFDSVADIYDSVFKKYVRDHYLEKRLKYIKRNFGNRKKILD
                     VGCGTGLLAMHLLQAGFDVYGVDVSPEMVKIAQKRLPGRIVCASVENIPFPDNSFDGV
                     ICIATLHHLSVTGFALAIKEMVRVLRIGGRLLIWDHNRINPYWLWLMKKVPQDTGRER
                     IFSAKNIMRQMKNTGLTMIKY"
BASE COUNT          713 a          417 c          534 g         1004 t
ORIGIN      
        1 tgtttcttca atttgttcag gttttgctct gagcagcgtc ctttcttatg gttatgtatt
       61 ttttactgtt attctgtcta tattttttgt tgcaaacgga gtacgcggta ttttaaaaaa
      121 tgttgcaatt gcctttatat gttttatctt ttcttatttt ctattgaatc cctatgtatt
      181 attgtctttt aatgagtttg ttaatgaact ttcgtacata caatcctact ggaggaactc
      241 tgtaacgctt gaaaatttaa agtatttttt gacaatgtcc ttacgatatg gccttggaac
      301 aggagtgtgg ttagtttctg tcattttttt gattttttct ttatattttt ccagggacaa
      361 gcaatttctt gtgatattgt taagtttatt acccgctgtg ttatattttg gaatcattac
      421 tggccgatgg gttcactatt cttttgttat ctatccatat atctttctta ttattctatt
      481 cggtgcaaca aaaattggag tgaaaggttc aatgctgata ctgttgcctg ttttaatatt
      541 tacatttata ttttctttcg cccatgtaac acttttttgc ggtaagaata ttcgtagcgt
      601 tgctggtgac tgggttaata aaaatattcc tgaaaattct tcaatgggtc ttctggaagc
      661 accatctcct tggaggactc cgccttttca atttttaaaa tatgatatca gaataggtac
      721 accagaacag atacaaaaac ctgaatactt tatcgtgagt caatatcaat ggttgagagg
      781 aggttcgttc aaggatatca agaaccttct tgctgattat gaaattataa aagaatttag
      841 aaaagagccg gtgtttttag gactgaaatt cagacaatct gacaatattc cttatgattg
      901 gtgtcatccg aatcccatta tcttgatatg gaaaagaaaa attcaaaaat tctgattttg
      961 attttttttg tcgcttttgt tgcacggatt tgttttattt tgacacttga caattctgtg
     1021 gatgtatggg gggattggtg ggatgaactt ggctggaaac tcgcttctgg acagggttac
     1081 tgggtaaata atccatattt tgttgatgga gaaccttttt attcatggag agcacctggt
     1141 tttcctttgt ttcttgctgg tatttacaaa atttttgggc acagttttct tgccgcaaag
     1201 attggtcttg cctttataag ttcgttgact gcggtttttt tatttccact ggcggctttt
     1261 tttgttggca acagagcagc gatggcatgc gcggcattct atgctgtgta tcctgtagca
     1321 attttctgga caggttacct cgctcctgaa acccttacag catttttcgt tgttgtatta
     1381 acatttttat ctgttctggc agtgagaaca gacagattat tgatatgttt tgtttctggc
     1441 ataatatttg gctgtctctt tttaaccaga ccgctcacaa ttgttttatt tcctgttatt
     1501 cttgtttatt ttttgatcca taaacaaaag atcgttaaga aaagtttgtt attttgtact
     1561 ggttgtttga ttgtttttcc ctggattgtg cgcaattatc ttatacatca cacacttgta
     1621 cttggttcga cagaaggcgg ggttgttttt tacatagcca ataacgaaaa agtattaggg
     1681 gagccaagtg gtttttatca tgctgaaaat atcacagaat tcagagggct ttctgaggtt
     1741 gaaatagata gaaaattcta tcgtatggga tttgattttg ttaagaaaaa tccatctcta
     1801 tattttaaac ttgtaatgga tagatttgtt agattctggc gtttcttccc acatacgatt
     1861 tcgggacctg gagaacccta tagcaaaaag catgttatta taagtgtttt tgttgaaact
     1921 ccattgatta ttctgggctg gattggtttt ttcctatctt ttcgacaatg gaggaatttt
     1981 tttgtattgc atggattcat tgtcacattt tcaactttta caatcctgat tcgtactgct
     2041 attcgatata gatttcctgt aatgccgctg atgcttgttt ttgcgattta tacattgtgg
     2101 cttttcaaga aattcaggga gaaccatgcc aggaaatttt gattcggtcg ctgacattta
     2161 tgacagtgtg tttaagaaat acgtccgtga tcactatctt gaaaaaagac ttaagtacat
     2221 taaaaggaat ttcggaaaca gaaaaaaaat ccttgacgtt ggttgtggaa cagggctgct
     2281 tgccatgcat cttttacaag cagggtttga tgtatatggt gttgacgttt ctccagagat
     2341 ggtgaaaatt gcacaaaaaa gactgcctgg tagaattgtg tgtgcatccg tggagaatat
     2401 cccatttcct gacaacagtt ttgatggtgt aatatgtatt gcaacattac accaccttag
     2461 tgtaacagga tttgctcttg ccataaagga aatggttcgt gttcttagaa ttggaggaag
     2521 attactgata tgggatcata acagaattaa cccttactgg ttatggttga tgaaaaaagt
     2581 tccccaggat accggacgcg aaagaatatt ttcagcaaag aatattatga ggcagatgaa
     2641 aaatacaggt ttaaccatga tcaaatac
//
LOCUS       DAORYU010000205         2655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_25810_length_2655_cov_3.328846, whole genome shotgun sequence.
ACCESSION   DAORYU010000205 DAORYU010000000
VERSION     DAORYU010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25810_length_2655_cov_3.328846"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PLW07_06260"
     CDS             <1..275
                     /locus_tag="PLW07_06260"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOL50166.1"
                     /translation="AGIPFDKGVELAEKIKSMFNPTDDITMAQLALRWILDHDAVSTV
                     IPGATKIEQAISNARTSSIPPLDESVHQKLYQFYKTEIEPYIRGRY"
     gene            complement(278..1561)
                     /locus_tag="PLW07_06265"
     CDS             complement(278..1561)
                     /locus_tag="PLW07_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50167.1"
                     /translation="MSLQKNNSKFEDIKSQDIVILRNLAAEISEIAHLPVHSTIISQW
                     ERTNDLKPGRPVVWINEVPWHEIQSCGLLNIQCENEVARRIEFELKTLLYMWHNARVD
                     MIVEPVYRLPVVIYDTGFGISPEAEYAGSQSSGIVSRHFVPQIKDENDIKKIKLPEIS
                     VDWDETKKRKKILEEIFSKYLTVELYGIGAIWFAPWDWLVMLVGVEETLLSLATRPSF
                     AHALTERFLEASLYRLEQFEKLKLLSPNNGNFRIGSGGFGYTSDLPKLPFSALGISST
                     EQWGFATSQIFSEVSPAMHKEFALNYEKKWLEKFGLSYYGCCEPLHNKIHILEEIGNL
                     RKISISPRADVKIAAEKIKNRYVISYKPNPAILACEQWDNEFVRNELQKNLTVMKENG
                     CIVEVIMKDISTIRHQPSRLTEWAKIASEVVHQFV"
     gene            complement(1530..1805)
                     /locus_tag="PLW07_06270"
     CDS             complement(1530..1805)
                     /locus_tag="PLW07_06270"
                     /inference="COORDINATES: protein motif:HMM:NF013067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50168.1"
                     /translation="MGAYLAVLGNVDGIVFTAGIGENQPIVREKSLEGLEQYGIVVDK
                     QKNHNIRGKESEISTPDSKIKVFVIPTNEELAIAIDTYEFAKKQFKV"
     gene            complement(1763..>2655)
                     /locus_tag="PLW07_06275"
     CDS             complement(1763..>2655)
                     /locus_tag="PLW07_06275"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HOL50169.1"
                     /translation="GLIEKIGAPDAIVKYSKDGFSINNTQEIPDHNHGLEIIAKLLVD
                     PSCGAVKNLNEIKGVGHRVVHGGEEFTGSMLIDEKVTNKIKEFIPLAPLHNPPNFQGI
                     VAAEKYFPESVQVACFDTAFHTTLPPVAYLYALPYDFYEKYRIRRYGFHGTSHRYVAR
                     RFAQILGKHKYDVNLITCHLGNGCSITAVKNGRSIDTSMGFTPLEGLVMGTRSGDIDP
                     GVILYFIENLKMNPSEVNKILNKESGLIGISGVSNDFRSIVESASKGNKMAQLAIEIF
                     SYRGKNTLALILRFLVMLME"
BASE COUNT          788 a          560 c          452 g          855 t
ORIGIN      
        1 ttgctggcat tccatttgat aaaggagtgg aactggctga aaaaattaag tcaatgttta
       61 atccgactga tgacatcaca atggctcaac ttgctttacg atggattctt gaccatgatg
      121 ctgtttcgac agtaattcct ggagcaacaa aaattgagca ggcaataagc aatgccagga
      181 cgtcatctat tccacctctt gacgaaagtg tgcatcagaa actataccag ttttacaaaa
      241 ctgaaattga accttatatc agaggaaggt actgaagtta cacaaattga tggacaactt
      301 ctgaagcaat ttttgcccat tcagtcaatc ttgatggctg gtgcctgatg gtgctgatat
      361 ccttcattat tacctcaacg atacaaccat tttctttcat aactgttaga tttttctgca
      421 attcatttct tacaaactcg ttatcccact gttcacacgc aagaattgct ggatttggtt
      481 tgtaggaaat cacatatcta ttttttattt tttctgctgc aattttcaca tccgcacgag
      541 gactgatcga aatttttcgc aaatttccaa tttcctcaag gatatggatt ttgttatgaa
      601 gtggttcaca acatccgtaa taagaaagcc caaatttttc aagccatttt ttctcataat
      661 tcaatgcaaa ctccttatgc atggcaggtg aaacttcaga gaaaatctgg cttgtcgcga
      721 aaccccactg ctctgttgat gaaatcccaa gagcggaaaa cggcaatttt ggaagatcag
      781 atgtataacc aaatccgccg gaaccaatcc tgaaatttcc attatttggc gaaagaagtt
      841 tcagtttttc aaactgctcc aacctgtaaa ggcttgcttc aagaaatctt tcagtaagag
      901 catgcgcaaa ggaaggtctt gtcgccaaag acagcagtgt ttcctcaaca ccaaccagca
      961 tcaccagcca atcccatgga gcaaaccata tcgctcctat gccatataat tcaactgtta
     1021 aatatttgct aaaaatttct tcgagtatct tcttcctttt ttttgtttca tcccaatcaa
     1081 cagaaatttc aggcaatttt attttcttaa tatcattttc atcttttatc tgtggaacaa
     1141 agtgccttga aacaattcct gatgattgag aaccagcata ttctgcttca ggtgatattc
     1201 cgaaacctgt atcataaatt accactggaa gcctgtaaac aggttcaaca atcatatcta
     1261 ccctggcatt gtgccacatg taaagaagtg tcttcagttc aaactcaatt cttctggcaa
     1321 cctcattttc gcattgaatg ttaagtaaac cacaggactg aatttcatgc catggaactt
     1381 cattaatcca gaccacgggt ctacctggtt tcaagtcatt ggttctttcc cattgtgaaa
     1441 taattgtaga atgaacaggc agatgcgcga tttctgaaat ttctgcggca agatttctaa
     1501 gaattactat atcctggctt tttatgtcct caaactttga attgtttttt tgcaaactca
     1561 tatgtatcaa tggctatcgc aagttcttca tttgtgggta ttacaaaaac ttttattttt
     1621 gaatcagggg tactaatctc agattctttt cccctgatat tgtgattttt ttgtttatcg
     1681 acgactatac catattgctc aagaccttca agagattttt ccctgacaat cggctggttt
     1741 tctccaatcc ctgcagtaaa aactattcca tcaacattac caagaaccgc aagataagcg
     1801 ccaatgtatt ttttcccctg tatgagaaaa tttctatggc aagttgtgcc attttattac
     1861 cttttgaagc gctttcaaca atacttctga aatcgtttga aacacctgaa ataccgataa
     1921 gtccactttc tttgtttaaa attttgttaa cctctgatgg gttcattttg agattttcaa
     1981 taaagtacag aattacaccc ggatcaatat ctccgcttct tgttcccatc acaagtcctt
     2041 caagtggagt aaatcccatg cttgtatcaa tagaccttcc attctttaca gcagtaatac
     2101 tgcaaccatt accaaggtgg catgtaataa gatttacatc gtatttatgt ttacccagaa
     2161 tctgagcaaa tcttctcgca acatacctgt gagatgtgcc atgaaaacca tatcttctaa
     2221 tccggtattt ttcataaaaa tcgtaaggca atgcataaag ataagcaaca ggaggcagtg
     2281 tcgtatgaaa agcagtatca aaacaggcaa cctgaacgct ttcagggaaa tacttttctg
     2341 cagcaacaat gccctgaaaa tttgggggat tgtgaagtgg agcaagagga ataaattctt
     2401 taatcttgtt ggtaaccttt tcatctatga gcatgctgcc agtaaattcc tcgcccccat
     2461 gcactaccct gtgaccaact cctttgattt cattgagatt ttttactgca ccgcaggaag
     2521 ggtctacaag aagtttcgca ataatttcca ggccatgatt gtggtcagga atttcttgag
     2581 tgttgttaat tgaaaaaccg tcttttgaat atttcacaat ggcatcagga gcgccaattt
     2641 tttcaatcag tccta
//
LOCUS       DAORYU010000206         2650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_25870_length_2650_cov_6.997303, whole genome shotgun sequence.
ACCESSION   DAORYU010000206 DAORYU010000000
VERSION     DAORYU010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25870_length_2650_cov_6.997303"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="PLW07_06280"
     CDS             complement(<1..791)
                     /locus_tag="PLW07_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HOL50170.1"
                     /translation="MNRFISFLIALVFSATTVLFAQNQAKSVIQIWMWGGPSQNDTFD
                     PKVAAGYDYCGPLDKTIPTNAAGIEINASLPMLAKIADKYSIIRSMTHGINSHESAAY
                     YVQTGHKPGPGLVYPSLGAIVSLFKGKNYTGKIPPYIVLTQSQGRFSEEGFLGPLYKP
                     FVTGGDPNQKEFAVEGIVAPGISEQQQKKRRELLQKLDTLGNAIGDEPDFVQFKKSED
                     EAYEMILGQARKVFDLSQESDEVRNRYGRNWFGQCCLVARRLVEIG"
     gene            complement(806..2434)
                     /locus_tag="PLW07_06285"
     CDS             complement(806..2434)
                     /locus_tag="PLW07_06285"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HOL50171.1"
                     /translation="MIYLYCESMMNPLKKIIATGLRNFLKNCLFEFLIIELCCVYIGF
                     AGIKPYEDDSWTTPENIIDLQVKSCLARYDATFRNPCSDYVFIRRVYLDTIGTLPTPE
                     EIDAFIKDTRQDKRKILIDNLLDRDEFVLYWTMKWCDILRVKSEYPINLWPNAVQAYH
                     RWIYEQIKKNLPYDQFVRGLLTSSGSNFRVPQVNFYRAIQGRSPLSIAKAVAMTFMGI
                     NLDNLPKDTQENMALFFSRVAYKKTLEWKEEIVYFNPEPGKTIRTSFLDGRSVEILPQ
                     QDGRIVFADWLISPKNPYFNKNIVNRVWAWLMGNAIISPVDDTYGKKTPGQEVLTVLE
                     QELVKSGYDIKHLIRLILNSRTYQQSSIPTLQTKNPDIQFAFYRIRQLEAEVLLDALC
                     WIGGMGEEYVSQIPEPYTFIPKENRNIGLADGSITSSFLITFGRPSRDTGLASERNSN
                     PTDKQRLYMLNSSDIQKKLRSSPLLRKALSQMWKNRKDCITLVYKIILSRPPTDQEMN
                     IIEEYLTNSGVSINQAATDIAWALINSKEFLFKH"
BASE COUNT          740 a          535 c          506 g          869 t
ORIGIN      
        1 ccaatttcaa caagtcgtct tgcaacaagg cagcactgac caaaccagtt tctgccatac
       61 ctatttctca cttcgtcgga ttcctgagaa aggtcgaaaa cctttcgcgc ttgccccaga
      121 atcatttcgt atgcctcatc ttcactcttt ttgaattgaa caaaatcagg ttcatcgcca
      181 attgcgtttc ccagagtatc taatttttga agcaattctc tccgtttttt ttgctgttgt
      241 tcagatatgc caggagcaac aattccttca accgcaaatt ctttttgatt cgggtctccg
      301 cctgtgacaa atggcttgta cagtggacca agaaaccctt cttctgaaaa tctgccctga
      361 gattgcgtaa gaacaatgta cggcggaatt ttgccagtgt aatttttccc tttaaacaaa
      421 ctgactattg caccaagaga agggtaaaca agtcctggac ctggtttatg tcctgtttga
      481 acataatatg ctgctgattc atgactattg ataccatgtg tcatgcttct gataatagaa
      541 tatttatcag caattttcgc cagcatgggc aaagacgcat tgatttctat tccagcagca
      601 tttgttggta ttgttttatc aagaggaccg caataatcat agccagcggc aactttcggg
      661 tcaaatgtat cgttctgaga aggtcctccc cacatccata tttgaatgac agattttgcc
      721 tgattctgcg caaacagaac agtggttgca gaaaaaacca gcgcgattaa gaatgaaata
      781 aatcgattca cgttcacatc tccttttaat gtttgaataa aaattccttg ctatttatca
      841 atgcccatgc aatgtctgtg gctgcttggt taattgacac tcctgaattt gtcaaatatt
      901 cttcaatgat gttcatttcc tggtctgttg gcggtctgga tagtataatt ttataaacaa
      961 gggtaataca atcttttctg tttttccaca tctgggacaa tgcttttctt aacagaggac
     1021 ttgaccttaa ttttttctgt atgtcgcttg aattcaacat atacagtcgt tgcttatcag
     1081 ttgggttgct gtttctttct gatgcaagac ctgtatccct tgatggtctt ccaaatgtga
     1141 tgagaaaaga actggtgata ctgccatctg ccagtccaat atttctattt tcttttggaa
     1201 taaaggtata tggttctggt atctgactaa catattcttc gcccatgccg ccaatccagc
     1261 acaaggcatc cagaagaacc tctgcttcaa gttgtctaat tctgtaaaat gcaaattgaa
     1321 tgtcagggtt ttttgtttgc agagttggta tcgaagattg ctggtatgta cgagaattga
     1381 gaattaatct tatcaaatgc ttgatgtcat agccagactt gaccagttct tgctccagaa
     1441 cagtaaggac ttcctggccg ggtgtttttt tcccatatgt gtcgtcaaca ggcgaaatga
     1501 tggcatttcc cattaaccat gcccataccc tgttaacaat atttttgttg aagtatggat
     1561 tcttaggaga gataagccaa tcagcaaaga cgatacgacc atcctgttgg ggcaaaattt
     1621 caacacttct gccatccagg aaacttgttc taatagtttt ccctggttct ggattaaaat
     1681 aaacaatttc ttctttccat tccagtgttt ttttgtacgc gacacgagaa aaaaacagtg
     1741 ccatgttttc ctgagtgtcc ttaggcagat tatcaagatt tattcccatg aaagtcattg
     1801 cgacggcttt tgctatggac agcggactac gaccctgtat tgctctataa aaattaacct
     1861 gaggcactct gaaattgctt ccgcttgatg tgagaagtcc gcggacaaac tggtcataag
     1921 gaaggttttt cttgatttgc tcataaatcc agcgatggta cgcctggaca gcattaggcc
     1981 atagatttat gggatattct gacttgacgc gaagtatatc gcaccacttc attgtccaat
     2041 acagaacaaa ctcatccctg tcaagaagat tatctatcaa tatctttcgt ttgtcctgcc
     2101 gtgtgtcttt tatgaaagcg tctatttcct caggggtagg aagtgttccg atcgtgtcaa
     2161 gataaacacg tctgataaaa acatagtcag aacatgggtt tcgaaaagtt gcgtcatatc
     2221 tggcaaggca ggacttcact tgtaaatcaa tgatattttc tggtgtggtc caggagtcat
     2281 cttcatacgg tttaatacca gcaaaaccaa tgtatacgca acacaattca attattaaaa
     2341 actcaaacaa acagtttttg agaaaatttc tgagccctgt cgcgataatt ttttttaatg
     2401 gattcatcat actttcacaa tacaagtata tcatacgtcg tttctttgtc ctggttcttg
     2461 caaaaattag acaatgttat ccgttgaagg tttatgtttg tttgaaaggt gttataatta
     2521 ttacaatcca cagggggtat aaatgctttg ccaaaaaatt tttcttatag tagcaatatt
     2581 gatgatgttt tttgatgtcc atattccgat ttgtaatgga aatactgata agatagtaag
     2641 cggaccgatc
//
LOCUS       DAORYU010000207         2640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_26013_length_2640_cov_3.472727, whole genome shotgun sequence.
ACCESSION   DAORYU010000207 DAORYU010000000
VERSION     DAORYU010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26013_length_2640_cov_3.472727"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="PLW07_06290"
     CDS             complement(<1..422)
                     /locus_tag="PLW07_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50172.1"
                     /translation="MKKIILFLLLSWLFVFSAIADSAIDYFNVMGEVYFRFENARVFN
                     MNEISTVVSISHVKDQWIYAYANQKEFARFLSLNIPFEMLQHPGYAANVDSALKFSLT
                     FTLSQYYPTYPEYLAMMQQFASDYPDICSVYDIGTSVQG"
     gene            complement(546..698)
                     /locus_tag="PLW07_06295"
     CDS             complement(546..698)
                     /locus_tag="PLW07_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50173.1"
                     /translation="MAFFKTGIHWPGSGHEKTAEAELKRIKNSKRKFLDALWQIIVLS
                     PSFMKI"
     gene            685..2364
                     /locus_tag="PLW07_06300"
     CDS             685..2364
                     /locus_tag="PLW07_06300"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50174.1"
                     /translation="MKNAINLQDPTGDASGSRDVVGNTTYPAAFIYNDGSYIYFRLRL
                     NSSPEGRGGQGFLQPFGWGLLLDTNLNNANYEWMIMVDGISQTEVINLWQNTVQGNVG
                     SPSDAPEILAYSISLNGNYQISLADSSFSGNPDYFLDWRFPYNIFKQYTGLTDYSPLR
                     LFFGSSSSTNTLTADLVGASDLYAGFTDYITPFGTKPTTGTVRFVADLTGNGDITQQC
                     VVNTIYIRVDDADQNKNPVAIDTVQITLSVPGGDSENLVLLETGNNTGIFTGSIFLTY
                     DNIVSGDQILQVQIGSTITATYIDNIDANLQLNQVRTDTFVVCGPILQVTKTVAPSNV
                     TAGETVVYTITIQNTGTGAAFINQIQDVLPSGFSFVNGTTTGITSNNPSINGQILTWN
                     GSWSIPAGSSNSLIFSAHAASVPGTYFNNVSVSGTNFPAFHTGDTAPVSIGAPVMTLT
                     KTADKTSAAPGEEITYTIYYKNTGYGQASFVIITDTIPPNTNYVGGSLRKGNASSTYG
                     SASPLTDAADSDEGYISGNMVIFNIGNVGPNDGVPDTGNDEGKVYFKVKID"
     gene            2404..>2640
                     /locus_tag="PLW07_06305"
     CDS             2404..>2640
                     /locus_tag="PLW07_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50175.1"
                     /translation="MVNLKKLFFATICVVVCATVLVAQEVSPKLVLVSKVEKEIKAEK
                     NGKQIIERVPADNVKTGDVLIYTITYKNEGNDVAQ"
BASE COUNT          836 a          523 c          502 g          779 t
ORIGIN      
        1 ccctgaacac ttgttcctat gtcatacaca ctgcaaatgt ccggataatc cgatgcaaac
       61 tgttgcatca ttgcaagata ttctggatac gtggggtaat actgcgacaa tgtgaaagtc
      121 aatgaaaatt tcaaagcaga atcaacatta gcggcatagc caggatgctg aagcatttca
      181 aatggaatat tcaacgataa aaatcttgca aattccttct ggtttgcata ggcataaatc
      241 cattgatctt tcacatgtga aatggaaacc actgttgaaa tttcattcat attgaaaacg
      301 cgggcatttt caaacctgaa atacacttca cccatcacat taaaatagtc aatagcagaa
      361 tctgctatgg cagaaaaaac gaaaagccag gatagcaaaa gaaataatat gatctttttc
      421 atattcgcaa acatccttat cataatattc actattacat ctaatcatat attggcattt
      481 gtttccagtt acatgaaaat ctggacccgg gaacataaac ttgccatcta aataccaaaa
      541 ggttattata ttttcataaa agaaggagaa agtacaatta tttgccatag tgcatcaaga
      601 aattttcgtt tgctgttttt tattcttttc agttctgctt ctgctgtctt ttcatggcca
      661 ctaccaggcc agtggatgcc tgttttgaaa aatgccatca atttacagga tcctactggc
      721 gatgcctctg gttcaagaga tgttgttggt aatactacct atcctgctgc atttatctat
      781 aatgacggct catatatcta tttcaggtta aggttaaatt cttctcctga aggcagagga
      841 ggacagggtt tccttcaacc attcggctgg gggcttcttc tggacacaaa cttaaataat
      901 gctaactatg aatggatgat tatggttgat ggaatcagcc agacagaagt cattaatttg
      961 tggcaaaata ctgtccaggg gaatgtaggg tctccgtcag atgctcctga aatacttgcc
     1021 tattccattt ctttgaatgg aaattatcaa atttctcttg ctgattcgtc tttcagtgga
     1081 aatccagatt attttcttga ctggagattt ccttacaata tcttcaaaca atacacagga
     1141 cttactgatt attcgccttt acgactattt tttggcagtt ccagcagcac caataccctg
     1201 acagcagacc ttgttggggc atcagattta tatgcaggat ttaccgatta tattacacca
     1261 tttggaacaa aacccacaac tggaacagtt cggtttgtcg cagaccttac tggtaatggc
     1321 gatattactc aacaatgcgt tgtcaatact atttatataa gggttgatga cgcagaccag
     1381 aacaaaaacc ctgtagccat tgacacagtt cagattaccc tttctgttcc tggtggagac
     1441 agtgaaaatt tagtcctttt agaaacaggt aataatactg gcatatttac tggctcaata
     1501 tttttgacat atgacaacat tgtttctggt gatcaaatct tgcaggtaca gattggttca
     1561 actattacag caacgtacat tgacaatata gacgcaaatc ttcaattaaa tcaggtcaga
     1621 actgatactt ttgttgtgtg cggaccaatc ttgcaggtga caaagaccgt tgcaccttca
     1681 aacgttacag caggtgaaac agttgtttac actattacca ttcaaaacac aggcactggc
     1741 gcggctttca tcaatcagat tcaggatgta ctaccatctg gattttcctt tgtcaatggc
     1801 acaacaacag gtatcacgtc aaacaatcct tctatcaatg gccagattct cacatggaat
     1861 ggttcttggt caatacctgc tggatcaagc aattccctga tattcagtgc ccatgcagca
     1921 tctgtccctg gcacatattt taataatgtt agtgttagtg gcaccaactt tcctgcattc
     1981 cacacaggcg atacagcacc ggtgtccatt ggagcgcctg taatgaccct gacaaaaaca
     2041 gcagataaaa cctctgcagc ccctggcgag gaaattactt acaccatcta ttacaaaaac
     2101 actggatacg ggcaagcaag ttttgtcatc atcactgata caataccgcc aaacacaaat
     2161 tatgttggtg gcagtttgag aaaaggaaat gcttcttcaa cttatggtag cgccagtcca
     2221 cttactgatg ctgcagattc agatgaagga tatatcagcg ggaatatggt tattttcaat
     2281 attggaaatg taggaccaaa tgatggagta cctgatactg gcaatgatga aggaaaagtt
     2341 tatttcaagg taaagataga ttaacaattt tgtttctgga ttacagagaa aaggaggtga
     2401 aacatggtca atctgaaaaa actgtttttt gccacaatat gtgtggttgt ttgtgccaca
     2461 gtacttgttg ctcaggaagt atctccaaaa cttgtgctcg tttcaaaggt tgaaaaagaa
     2521 ataaaggcag aaaaaaatgg caaacaaatc attgaaagag ttcctgcaga caacgtaaaa
     2581 actggagatg tccttattta cacaatcaca tacaaaaatg aaggtaatga tgtggcacag
//
LOCUS       DAORYU010000208         2631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_26123_length_2631_cov_4.024845, whole genome shotgun sequence.
ACCESSION   DAORYU010000208 DAORYU010000000
VERSION     DAORYU010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26123_length_2631_cov_4.024845"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="PLW07_06310"
     CDS             complement(<1..478)
                     /locus_tag="PLW07_06310"
                     /inference="COORDINATES: protein motif:HMM:NF012868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HOL50176.1"
                     /translation="MKPSFFALGTIFVPVLGCLAIPLASLVSTRLRNLVALLLGFATF
                     ILPIFLIPAVLQSPEGQIVFRYPSILNLNFILLIDGLSVFVALVSSFIGALIILYSID
                     YVAHNEFQTEYYTMVVMFIGSMMGLVFSGNLIFMYLFWEIAAICSWRLIGFYRGPSH"
     gene            complement(529..1014)
                     /locus_tag="PLW07_06315"
     CDS             complement(529..1014)
                     /locus_tag="PLW07_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019691058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HOL50177.1"
                     /translation="MQVITVKIEKPESINFILGQTHFIKSVEDIHEALVSTVPGIKFG
                     LAFCEASGKCLVRWSGTDSEMIKLAKTNAERIGAGHSFIIFLGQGFYPINVLNTIKMI
                     PEVCCIFCATANPVEVVIVETQQGRGILGVIDGFSIKGVEDEEEIKWRKDLLRKIGYK
                     F"
     gene            complement(1038..2138)
                     /locus_tag="PLW07_06320"
     CDS             complement(1038..2138)
                     /locus_tag="PLW07_06320"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOL50178.1"
                     /translation="MTPRERLIRVFKCLDVDRMPIRLWGVDPMFPVRDDWQPLIELTE
                     KYQLEIIRTWSPSKEEVPESVCKQCDTERIIEERSIREITTTIETPAGLLTRIFHQPI
                     SGAPGYVRKHCVENIEDAKKWLSIPDDELPVVDSYFELEKKTGSRAMLMVGIDEAMYM
                     VQRLMGSEVFGYWLYDERDLLREMIDRAYKKIERLVKHYLSKGLGDAYGWVGPELCIP
                     PLASVKDFYEFVVEYDRKIIDLVHESGKLTWIHCHGDMKPVLEGFINMGLDCLNPIEP
                     PPVGKITLAEAKKICKGRMCLEGGVEDGAFYTKTPQEIKEMTINTINQGKPDGGFILC
                     PSSSPNTAAILPPYVIENYRIFVETAVRLRNY"
     gene            complement(2141..>2631)
                     /locus_tag="PLW07_06325"
     CDS             complement(2141..>2631)
                     /locus_tag="PLW07_06325"
                     /inference="COORDINATES: protein motif:HMM:NF024757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta galactosidase jelly roll domain-containing
                     protein"
                     /protein_id="HOL50179.1"
                     /translation="NGETGLLVSLAPKNVKFAIDEYGLGKHFRWYLPVFDKSKWKLID
                     TTKPFYIQGYLSSDGMPYMGKMWYVFDLDVPKNFAGKQMKLYAPFVTCEAWVWVNGQY
                     AGHRKYLEAYISPAEIDMDITSFVKPGEKNTIAVWVSTGLSPAQAADGFLGRLFLYSP
                     VK"
BASE COUNT          838 a          598 c          419 g          776 t
ORIGIN      
        1 tatgagatgg tccacgatag aaaccgatca atctccatga acatattgct gcaatctccc
       61 agaaaagata cataaaaatc agattgccag aaaacacaag ccccatcatt gaaccaataa
      121 acattactac cattgtatag tactctgttt gaaactcatt gtgcgcgaca taatcaatgg
      181 aatacaaaat aattaatgcg ccgataaaag atgaaacaag cgccacaaaa actgacaatc
      241 catcaatgag caaaataaaa ttgaggttca ggatagatgg gtatctgaaa actatttgac
      301 cttcagggct ctgaaggact gccgggatca aaaaaatggg taaaataaac gtggcaaacc
      361 caagaagcaa agccacaaga ttcctcaacc tggttgatac caaagaagcc agtgggattg
      421 cgagacaacc aagtaccggc acgaaaatcg tcccgagagc aaagaaagat ggtttcatac
      481 gggctattag tttaatcaaa aattatgata ctgtcaaaaa attcaaaact aaaacttata
      541 tccaattttc cggagcaagt ccttccgcca ttttatttct tcttcatctt ctacaccttt
      601 tattgaaaat ccatcaatta caccaaggat accccttccc tgctgggttt caacaattac
      661 cacttcaaca ggatttgcag ttgcgcaaaa aatacaacac acttcaggga tcatttttat
      721 cgtattcaaa acatttattg gataaaaacc ctgtccaaga aaaatgataa aactatgccc
      781 ggcaccaatc ctttcagcat ttgtttttgc aagtttaatc atttcagaat ctgttccgga
      841 ccatcgaaca agacatttac ctgaggcctc acaaaacgcc aggccgaatt tgatacccgg
      901 caccgttgaa acaagtgctt cgtgaatgtc ttcaactgat tttatgaaat gtgtttgccc
      961 cagaataaaa ttgattgatt ctggtttttc aatttttaca gtgataactt gcatagtaac
     1021 cccctgttaa acatatttca ataatttcgt aatctaacag cagtttctac aaaaatcctg
     1081 tagttttcta ttacatacgg cggcaatatc gctgcagtat ttggactgga tgacggacag
     1141 agtatgaacc caccatctgg ctttccctga ttgattgtat taatcgtcat ttcttttatt
     1201 tcctggggtg ttttcgtata aaatgctcca tcttcaacac caccttcaag acacattctc
     1261 cccttgcaga ttttttttgc ttcagcaagc gtaatctttc caactggtgg tggttcaatt
     1321 ggattcaaac aatcaagtcc catattgata aatccttcaa gcacaggttt catatctccg
     1381 tggcaatgaa tccaggttaa ttttcctgac tcgtgtacaa ggtcaataat tttcctgtca
     1441 tattcaacaa caaattcata aaaatccttc actgaggcaa gaggcggaat acaaagttca
     1501 ggaccaaccc atccgtatgc gtctccgagt ccttttgaaa ggtaatgctt cacaagtcgt
     1561 tctatttttt tatatgccct gtcaatcatt tctctcagaa ggtctctttc atcataaagc
     1621 cagtaaccaa aaacctctga acccattaac ctttgaacca tgtacattgc ttcatctata
     1681 ccaaccatca acattgccct tgaaccagtt tttttctcaa gttcaaaata agaatcaact
     1741 actggaagtt catcatcagg aattgaaagc cattttttcg catcttctat attttcgaca
     1801 caatgctttc ttacatatcc aggagcacct gaaatcggtt ggtgaaaaat ccgggtaagc
     1861 aatcctgctg gggtttcaat ggttgtagtg atttccctta tacttctttc ttcaataatc
     1921 cgttctgtat cacattgttt acatacagat tctggaacct cttctttcga aggagaccat
     1981 gtcctgataa tttcaagttg atatttctca gtaagttcaa tcaatggttg ccagtcatcc
     2041 ctgacaggaa acattgggtc aacaccccac aacctgatgg gcatcctgtc aacatcaaga
     2101 catttgaaaa ccctgattaa tcgttctctc ggtgtcatct tcatttcaca ggagaatata
     2161 aaaacaacct tccaagaaat ccgtctgctg cctgagcagg gctcaatccg gtactgaccc
     2221 acacagcaat agtatttttt tctccgggtt taacaaaaga tgtaatatcc atatcaattt
     2281 cagcaggact gatgtatgct tcaagatatt ttctatgtcc ggcatactga ccattgaccc
     2341 acacccacgc ctcacacgta acaaaaggtg catacaactt catctgtttt ccagcaaaat
     2401 tttttggaac atcaaggtca aaaacatacc acatttttcc catataaggc ataccatctg
     2461 aagaaagata tccctgaata taaaagggtt ttgtcgtatc aattaatttc cacttgcttt
     2521 tatcaaaaac aggcagatac cacctgaagt gtttaccaag cccatattca tcaatagcaa
     2581 atttaacatt tttaggtgcc agagacacaa gtaatcctgt ttcaccattt g
//
LOCUS       DAORYU010000209         2624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_26209_length_2624_cov_3.892565, whole genome shotgun sequence.
ACCESSION   DAORYU010000209 DAORYU010000000
VERSION     DAORYU010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26209_length_2624_cov_3.892565"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="PLW07_06330"
     CDS             complement(<1..191)
                     /locus_tag="PLW07_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOL50180.1"
                     /translation="MGKIRLLNEETISRIAAGEVIERPASVVKELIENSIDAGATKII
                     VDIKQAGKTSIIVKDNGCG"
     gene            complement(184..>2624)
                     /gene="mutS"
                     /locus_tag="PLW07_06335"
     CDS             complement(184..>2624)
                     /gene="mutS"
                     /locus_tag="PLW07_06335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOL50181.1"
                     /translation="KAAGILDLVLTSRDAGKSGKIPMCGLPFHAADSYIARLIKHGYK
                     VAICEQTEDPSKAKGIVKREVIRVITSGTYLDDTAETRCIVSIVIDKNTIGIAIIDNT
                     KGNIRANQYQGIENFLNILTRMPVYEIIYPEANEKEIVELINKTFGRLKNFTLTGLED
                     WKFHYDTVMRNLCQHFKIHSLAGTGIGDKILAVRAAGALLEYITEMARGPLQHIDKIS
                     LYDDSEYVYLSPSAIRGLEIESLVKTIDRTQTASGKRLLREWLLHPLKNVEKINERLS
                     AVTILKENPSIQQNLIVIFKQAYDIEKSLSRLSSGYSNPRDLLAIKNTLTIVPQIKQV
                     LDPIASLSRFFSISDIENLRDFLDKSINDDIPLSNPEGQIIKKGFNDEIDSYREIKET
                     SNQWLKNYQSEEIKKTGIASLKIGYTQVFGYYIEISKPNLHLVPHDYIRKQTLVNAER
                     FITPQLKEFEEKMLSAQQRILEIENMLIKQIIEKILEHSNEIHQLANSIAELDVLNSF
                     SILANSSGYTKPEITDEDEIMIKDGRHPLVETFIGNNFIPNDTLVDCNTNRLLIITGP
                     NMAGKSTYIRQVALLVIMAQSGSYIPVSVAKIGVADKIFARIGAHDEIAKGQSTFMVE
                     MSETASILNNLSERSLVILDEIGRGTSTYDGLALAWAVAEYLHQHKIRTLFATHFHEL
                     VELARTHPCVRNYNVAVKQWQDEIIFLHKIVPGGTDQSYGIYVAKLAGVPDSIIQRSG
                     EILRGLESVEKFSISDNETEQLSLFINQPDPIGEQLKSEILNINPDNITPSEALNKIY
                     EWKKMLEKKGLKNG"
BASE COUNT          677 a          507 c          496 g          944 t
ORIGIN      
        1 ataccacatc cattgtcttt tacaataata ctggtctttc ctgcctgttt tatatcaaca
       61 attatttttg tggcaccagc atctatcgaa ttttcaataa gttcctttac cacagatgcc
      121 ggtctttcga ttacttctcc tgctgcaatt ctgctgatgg tttcttcatt cagtaggcga
      181 atcttaccca tttttcagtc cttttttttc aagcattttt ttccattcat agattttgtt
      241 taatgcttca gacggagtga tattgtcagg attgatattg agtatttctg attttaattg
      301 ttcgccaatc gggtcgggtt gattgatgaa aagagaaagc tgttcagttt cattatctga
      361 aatggagaat ttttcaaccg attcaagtcc ccttaaaatt tctcctgacc tctggattat
      421 actgtctggt actcccgcaa gttttgccac ataaatgcca taactctggt ctgtcccacc
      481 tggtacgatt ttatgaagaa atataatttc atcctgccat tgtttcactg ccacattata
      541 atttcttacg caagggtgag ttcttgccag ttctaccagt tcatgaaagt gtgtggcaaa
      601 aagagttctg attttatgtt gatgaagata ttcagccaca gcccacgcca gagcaaggcc
      661 atcataggta ctggttcctc tgccgatttc gtcaagaatt actaaacttc gttcagaaag
      721 attattgaga atagatgctg tttcagacat ttcaaccata aaggtgctct gcccttttgc
      781 gatttcgtca tgtgctccaa ttcttgcaaa aattttatcc gcaacaccaa tttttgcgac
      841 tgacacaggt atgtatgaac cgctctgtgc cataataaca agaagcgcaa cctgtctgat
      901 atatgtagat ttgccagcca tattagggcc ggtgataatg agcagccgat tggtattgca
      961 atctaccagt gtatcgtttg gtataaaatt gttcccaata aatgtctcaa ccagggggtg
     1021 tcttccatcc tttatcatga tttcgtcttc atcggtaatt tctggcttcg tgtaacccga
     1081 agagtttgca agaatactga atgaatttaa aacatccagt tctgcaattg aattggctaa
     1141 ttggtgaatt tcgtttgaat gctcaagtat tttttcaatg atctgtttta ttaacatatt
     1201 ttctatttca agaattcttt gctgtgctga cagcattttt tcttcaaatt ctttgagttg
     1261 tggtgtgata aatctttctg cattgacaag cgtttgcttt ctgatatagt catgcgggac
     1321 aaggtgaaga tttggtttac tgatttcaat gtaatagcca aacacctggg tatagccaat
     1381 tttcaaggat gcgataccgg ttttttttat ttcttcagat tgatagtttt ttaaccactg
     1441 gtttgaggtt tctttaatct cgcgatatga atcaatctca tcattaaatc ctttttttat
     1501 aatctggcct tctggatttg acagggggat gtcgtcattg atggatttgt caagaaaatc
     1561 ccttagattt tcaatgtcac tgattgagaa aaacctactc aatgatgcga tgggatcaag
     1621 aacctgtttg atttgaggta cgatggtaag agtgttttta atggcaagaa ggtctctggg
     1681 atttgaataa ccactgctga gtcgtgacag agatttttca atatcataag cctgtttgaa
     1741 aataacaatg agattttgct gtatggatgg attttctttg agtattgtta ctgctgacaa
     1801 tcgttcgttg attttctcca cattttttaa tggatgcaac aaccattctc tcagtaatct
     1861 ttttccgctg gctgtttgcg tcctgtcaat agttttgaca agtgattcaa tttcaagccc
     1921 tcttattgca gatggcgaga gatacacata ctctgaatca tcgtaaagag agattttatc
     1981 aatgtgttgc aagggacccc ttgccatttc tgtgatgtat tcaagcagcg cgccagcggc
     2041 ccgaacagcc agtattttgt ctccgattcc tgtgccggca agtgagtgaa tcttgaagtg
     2101 ctgacagaga tttctcatca cagtgtcata atgaaatttc cagtcctcca ggcctgtgag
     2161 cgtaaaattt ttcaatctac caaaggtttt gtttatcagt tctacaatct ctttttcatt
     2221 tgcttctgga tagatgattt cgtaaactgg cattcttgta agaatattta agaaattttc
     2281 aataccctga tactggtttg ctcttatatt tcctttcgta ttatcaataa tggcgatacc
     2341 gatggtgttt ttatcaatta cgattgaaac tatacatctt gtttctgctg tatcatcaag
     2401 atatgttcct gatgtaatga ccctgataac ctctcgttta acgattcctt ttgcttttga
     2461 tgggtcttct gtttgttcgc atatcgcaac tttgtatcca tgctttatca accttgctat
     2521 gtaagagtca gcagcatgaa atggtaatcc acacatggga atcttacctg attttcccgc
     2581 gtccctggag gtaagaacaa ggtcaagaat acctgctgct tttt
//
LOCUS       DAORYU010000210         2624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_26213_length_2624_cov_3.552744, whole genome shotgun sequence.
ACCESSION   DAORYU010000210 DAORYU010000000
VERSION     DAORYU010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26213_length_2624_cov_3.552744"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..2048
                     /locus_tag="PLW07_06340"
     CDS             168..2048
                     /locus_tag="PLW07_06340"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOL50182.1"
                     /translation="MAQNISVIKKKKHPTIDFAASQIKKYLKKAGLTQCIQIILDIEN
                     QSNEDTISIQPHNSGYFITGSNPRSVLFGVYRFLSELGFRWIRPGNRGEIIPDIKSIR
                     KNIRINEKASYKYRTLCIEGATSLQHILDLIDWAAKNGMNGYFIQFDYGTHFFKRWYS
                     HQDNPYMKPEKFDEDDARKAVEIITEEVKKRGMRLEKMGHGWTCRALGIEGEGWDPAK
                     NSISEIPEEKRNWIAMIDGKREFFKNVPLNTNLCYSNPSVRSAIADAIVEYAEKHPEA
                     DAIHLWLADGSNNNCECENCQKARVSDFYVQILNELDEKLTSRNISTKIVFLIYVDLL
                     WQPEIEKIKNPDRFIIMFAPITRSYIQSFANTKPEQGIKPYIKNKLEFPKNAADNMLY
                     LQKWQEMFKGEGVDFDYHVIWACYYDLNHFTIGNVLYRDIVYLKNMNLHGFISCQNQR
                     VSFPTNFLMDILAKTLWNRKISFEKIVQESFSDAFGTNDSKKVIDFLRKMSLLWKPMF
                     EPVFIPQPDEKRIAAGIKNIKTMEKLCAQFKPFVARKAKSTTGATGWSWKYLNYYLEL
                     LEVLLPAMLNYLQRTSECRQKFEQVFEFLRKNEKILHPTLDVSTYIKVLQWRVNEAEG
                     NT"
     gene            2045..>2624
                     /locus_tag="PLW07_06345"
     CDS             2045..>2624
                     /locus_tag="PLW07_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50183.1"
                     /translation="MTHRERVVAAIKGEPVDRVPADYYGTPEATQKYLKELNLKDEFE
                     LIQFLDTDVIRAVSGPGKVKFSREKEFMKNVTSTAEIRKLSKDIPPIDDLIDNTPIIE
                     ARKKHPEYAILTYGPGSLFLSNNSFFGYETSLLHHAARPDLIKEVIKCGVEYAIEVID
                     KFHRETGNAVDIVSLEDDFGTQTSLYISYEMFV"
BASE COUNT          951 a          493 c          521 g          659 t
ORIGIN      
        1 gaggaagaat tttgaaaagg gggagaacgg gggaggaatt ttgaaaagga gaaatataaa
       61 gagggaaaac cgcgaagtaa attgaaaaag caaaaaaata cttgacagaa aatgcgcccg
      121 ttatacaatt tattcaaaac aatttcagtg gctaagaaag gagaatgatg gctcaaaaca
      181 tttctgtcat caagaaaaag aaacacccga cgattgattt tgcagccagt cagattaaaa
      241 aatacctgaa aaaagcagga ttaacacagt gcattcagat tattcttgac attgaaaacc
      301 agagtaatga agacacaata tcaatccagc cacacaacag tggctatttc atcacgggct
      361 caaacccaag aagcgtgttg tttggtgtat atcgattcct ctctgaactc ggattcagat
      421 ggatacgacc aggaaaccgt ggagaaatca ttcctgatat caaaagcatc agaaaaaaca
      481 tcagaatcaa cgagaaggcg tcgtataaat acagaacctt gtgcattgaa ggtgccacat
      541 cattacagca catacttgac ctgattgact gggcagcaaa aaacggaatg aacggatact
      601 ttattcaatt tgactatgga acgcattttt tcaaacgttg gtacagccac caagataatc
      661 cttacatgaa gccagaaaaa ttcgacgagg atgacgcaag aaaagccgtt gaaatcatta
      721 cagaagaagt caagaaaagg ggtatgagat tagaaaaaat gggacatggc tggacatgcc
      781 gcgcgcttgg tatagaaggt gaaggatggg acccagcaaa aaactcaata tccgaaatac
      841 ctgaagaaaa aagaaactgg atcgcaatga tagacggaaa acgagaattt ttcaaaaatg
      901 ttccactcaa cacaaatctg tgttattcaa atccatctgt tcgttcagcc attgcagacg
      961 caatcgttga atatgcagaa aaacaccctg aagctgatgc aatccatctc tggcttgctg
     1021 atggctcaaa caacaattgc gaatgtgaaa actgccagaa agcaagggtc tctgatttct
     1081 atgtacaaat cctcaatgaa ctggatgaaa aacttaccag ccgaaatatt tcaacaaaaa
     1141 ttgttttcct tatctatgtt gaccttctct ggcaacctga aattgaaaaa ataaaaaacc
     1201 ctgacaggtt catcattatg tttgcgccca tcacgagaag ttatattcag tcatttgcaa
     1261 acacaaaacc cgaacaaggt ataaaacctt acataaaaaa caaacttgaa tttcctaaaa
     1321 acgcagcaga caacatgctc tatcttcaaa aatggcagga aatgtttaag ggagaaggcg
     1381 ttgattttga ttatcatgtt atctgggctt gctattatga cttaaaccac ttcacgattg
     1441 gcaacgtttt atacagggac attgtatacc ttaaaaatat gaatctgcac ggatttattt
     1501 catgtcagaa tcaacgcgta tcctttccga ccaattttct tatggatatt cttgcaaaga
     1561 cactatggaa caggaaaata tcttttgaaa aaattgttca ggaaagtttt tcagacgcat
     1621 tcggaacaaa tgactcaaaa aaagtgattg attttctcag gaagatgtca ttgctgtgga
     1681 aaccaatgtt tgaaccagtg tttattccgc agcccgatga aaaacggata gccgctggca
     1741 tcaaaaacat caaaacaatg gaaaaactgt gtgcacaatt caaaccattt gttgccagaa
     1801 aagcaaaatc cacaaccggc gcaactggct ggtcgtggaa atatttgaat tattatctgg
     1861 aacttcttga ggttttgttg cccgcaatgt taaattacct tcaaaggact tctgaatgca
     1921 ggcaaaagtt tgagcaggta tttgaatttc tcagaaaaaa tgaaaaaatc cttcacccta
     1981 cccttgatgt ttccacatat atcaaggtgc ttcagtggag ggtcaacgaa gcagaaggca
     2041 atacatgacg catagagaaa gggttgttgc agccatcaaa ggcgagccag tcgacagagt
     2101 ccctgctgat tattacggaa cacctgaggc aacccaaaaa tacctgaaag aacttaatct
     2161 aaaagatgaa tttgaactca ttcaatttct tgatacagat gtcatcaggg cagtatcagg
     2221 tccaggaaag gtaaaatttt caagggaaaa agaatttatg aaaaacgtaa caagcactgc
     2281 tgaaataaga aaactttcaa aggatattcc gccgattgac gaccttattg acaatacgcc
     2341 aatcattgaa gcaagaaaaa agcatcctga atatgcaata ctcacttatg gacccggttc
     2401 actctttctc agcaataaca gtttttttgg atatgaaaca tcccttttgc atcatgctgc
     2461 aagacctgac ctgattaagg aagtgataaa atgtggggtt gagtatgcca ttgaagtgat
     2521 tgataaattc cacagagaaa ctggaaatgc tgttgacatc gtttccctcg aagatgactt
     2581 tggaacacag acatcgctat acataagtta tgaaatgttt gttg
//
LOCUS       DAORYU010000211         2582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_26847_length_2582_cov_5.131381, whole genome shotgun sequence.
ACCESSION   DAORYU010000211 DAORYU010000000
VERSION     DAORYU010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26847_length_2582_cov_5.131381"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="PLW07_06350"
     CDS             <1..615
                     /locus_tag="PLW07_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50184.1"
                     /translation="VATDSTWKWYYGSNEQEKNAYEKFWGRIIRFLCKPEDYLKIGDI
                     VPEIFVDRIFAKGEKVDIKFVFKNQDKPFKAYVTGPDNLREDLHISQNTCSFVPDREG
                     VYVVCAETERKINKKEFVVTKQGSETENNGKDDIYLKKLAEISDGEYFPVENPEGLIK
                     SLKLRKTAIKTRLAVTDDSEKYLVFMIFLVLSLCWFLRRRNNIL"
     gene            650..>2582
                     /locus_tag="PLW07_06355"
     CDS             650..>2582
                     /locus_tag="PLW07_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50185.1"
                     /translation="MNETLDHIYGYLVAYRKIERKTRISEVVCIFLSAIALVFTILIL
                     LDRFLPFFSFYTNYCRIFVFLVIAGFVTWIFYTNWKCVYSPFDTVALKIEKKFPFLNN
                     LVINAVQISRKTGKYPEIFIEVLRKKAVQSLANCNIEQAIDRYKLRKIFIVAVVSLFF
                     LGMSSVLLPQSARNVLLKILMPGKFADSISVEPGNCAIERGSSLVIRAKVKEPGNPII
                     QIKDRTIKNEPMIQDGQIFVYTINEVITPFSYRVVSNNKSTLWYKVNVVEKTLIKKMK
                     LTLEYPAYTGLKPKTIEKEFSEISGLKGTNITAEFYFNNSVGDTLLVFGDGRSISNRG
                     TSRVKSFRFTINDTTFYQIHYQDLATKNTLSSPKERITLIFDQIPFCEFMSPGRDIVA
                     SPGNAIPVKLKVSDDFGINVIRFRIHTGEGEISSNDRIFYQSTGNGRKDLTVDALLKI
                     PAGYPKIAYYAECTDYSPAANTGRSSIYFIYSPQAAPKNLSTRRPTEEEKTQQQEMEQ
                     AKKLLEKFIEEQKKVIEAAKKLGKVKNAADPSELQKLAEQEKKWADMLQKIVNDLNKI
                     GQQTQGKFTLSDEFVEMISHLQAASDAFKRNKPITIPIQESQMGLEMAEELVANLERW
                     LAEAPDSVKWDHQEPSKPTL"
BASE COUNT          912 a          467 c          497 g          706 t
ORIGIN      
        1 gttgccactg attcaacatg gaaatggtac tatggttcaa atgaacagga aaaaaatgct
       61 tatgagaaat tctggggaag aatcataaga tttctctgta aaccagagga ttatttgaaa
      121 attggtgaca tcgtgcctga aatttttgtg gacagaatct tcgcaaaagg cgaaaaggtt
      181 gacatcaaat tcgttttcaa aaaccaggac aaacctttta aggcatatgt cacaggaccg
      241 gataatctac gcgaagatct ccatatatcc cagaatacgt gttcctttgt tcctgacaga
      301 gaaggtgttt atgtcgtctg cgctgaaacc gaaagaaaaa ttaacaaaaa agaatttgtt
      361 gtaacaaagc agggcagtga aacagaaaat aacggtaagg atgacatcta tctcaaaaaa
      421 cttgctgaaa tttctgatgg tgaatatttc ccggttgaaa accccgaggg cttgataaaa
      481 tcgttaaaac ttcgtaaaac tgcaattaaa acacgccttg cagtgacaga tgattcagaa
      541 aaatatctcg tttttatgat ttttctggtt ctttctttgt gctggttttt gaggaggaga
      601 aataatatac tatgagtgag atttcgtcta aaaaaagagg aaaagtatta tgaacgaaac
      661 actggatcat atttatggtt atcttgttgc ctacaggaag attgaaagga aaacaagaat
      721 cagcgaagtc gtatgtattt ttctttctgc tatcgcgtta gtatttacca tactgatact
      781 tctggacaga tttcttccgt ttttttcatt ttacacaaat tattgtagaa tttttgtttt
      841 tctggtcatt gctggatttg ttacctggat tttttacaca aactggaagt gcgtatattc
      901 tccttttgat acagttgccc tgaaaataga aaaaaaattc ccgttcctga ataatctcgt
      961 tattaatgct gttcagatca gcagaaaaac aggaaaatat cctgaaattt ttatcgaggt
     1021 gctgagaaaa aaagcagttc aatcccttgc aaattgcaat attgaacagg caattgacag
     1081 atataaactg agaaagattt tcattgtggc agttgtgtcc ctgttctttc tcggcatgtc
     1141 ttcagttcta ttaccacaat cagcccgaaa tgtgttgttg aaaattctca tgccaggaaa
     1201 atttgctgac agcatcagtg ttgaacctgg aaactgtgca atagaacgtg gttccagcct
     1261 tgtcatcagg gcaaaagtaa aggaacctgg caatccgata atacagataa aagatagaac
     1321 aataaaaaat gaaccgatga ttcaggatgg acagattttt gtttacacaa taaacgaagt
     1381 aatcactccc ttttcataca gggtagtttc aaataataaa agcactctct ggtataaggt
     1441 gaatgtggtt gaaaaaaccc ttattaaaaa aatgaaactt acccttgaat atccagcata
     1501 tactggattg aaaccaaaaa caattgaaaa ggagttttct gaaatcagtg gtctgaaagg
     1561 aactaacatt acagcagagt tttactttaa caattcagtt ggagatacac tactggtttt
     1621 tggtgatggt agaagcattt caaacagagg tacatcaaga gtaaaatctt tcagattcac
     1681 aattaatgac acgacctttt atcaaattca ctaccaggac ctggcaacaa aaaacaccct
     1741 gtcaagcccg aaagaacgaa tcacactcat ttttgaccag attcctttct gtgaatttat
     1801 gtcaccaggc agagacattg ttgccagtcc tggcaacgca attcctgtga aactgaaagt
     1861 atcagatgat tttggtatca atgttatccg attcagaata catacaggcg aaggtgagat
     1921 ttcaagtaat gacaggattt tttatcagag tactggcaat ggcaggaaag atttgactgt
     1981 tgatgcgctt cttaagattc cagcaggtta tccaaagatt gcatattacg cagaatgcac
     2041 tgattattca ccagcagcca ataccggacg ctcatcaatc tatttcatct attcgcctca
     2101 agcagcgcca aaaaatcttt ctacccgcag acccacagaa gaagaaaaaa cacagcagca
     2161 ggaaatggaa caggcaaaaa aactccttga aaaattcatt gaggaacaaa aaaaagtcat
     2221 tgaagccgca aaaaaactgg gaaaagtaaa aaatgccgca gacccttcag aattacaaaa
     2281 actggctgaa caggaaaaaa aatgggcaga catgcttcag aaaatagtaa acgacctcaa
     2341 taaaatcgga cagcaaacac agggaaaatt tactctttca gatgaatttg ttgaaatgat
     2401 atcacatctt caggcggctt cagatgcatt taaaagaaac aaaccaatta caataccaat
     2461 acaggaatct caaatgggtc ttgaaatggc agaagaactg gtggcaaacc ttgaacgatg
     2521 gctcgctgaa gcgcctgact cagtaaaatg ggaccatcag gaaccatcaa aaccaaccct
     2581 tg
//
LOCUS       DAORYU010000212         2569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_27056_length_2569_cov_3.806285, whole genome shotgun sequence.
ACCESSION   DAORYU010000212 DAORYU010000000
VERSION     DAORYU010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27056_length_2569_cov_3.806285"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /gene="obgE"
                     /locus_tag="PLW07_06360"
     CDS             complement(<1..684)
                     /gene="obgE"
                     /locus_tag="PLW07_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOL50186.1"
                     /translation="MIDFVDEARIWIKAGDGGPGCIAFRREKFVPKGGPSGGDGGKGG
                     SVYFRACSNVKTLREYYYHRHFKAESGKPGEGNNRTGADGKDIILKVPPGTVIIEIRE
                     GEKEVMLADLDRDGNQVLAARGGKGGKGNASFKSSTNQTPRIATPGASGEEKFIKLEL
                     KLIAEVGIIGSPNAGKSTLLSKISNARPKIADYPFTTLNPNLGVVVLDNERQFVAADI
                     PGLIEGASLG"
     gene            complement(677..2317)
                     /locus_tag="PLW07_06365"
     CDS             complement(677..2317)
                     /locus_tag="PLW07_06365"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HOL50187.1"
                     /translation="MNILGVRKKYRLASRVSFVLRTIVRHGLGYFIDRIFREPHFRIL
                     RFHRASASRKKESLSTAERLRCVIEELGPTYIKIGQFFSTRADILPENFLKELSKLQD
                     NVSPFPFEAVKKTIVEDFGKQVEEIFAEFSEQPQFSASIGQVHFARLLSGKECVVKVR
                     RPGVEEVVAADIDVLKEILRLASKHIPELKQWNISDLLEEVGELLRRQMDFIYEAGMM
                     EKLKVFYEKTDIRVPHVLWEYTSGRVLTMEQIHGVKILDVSLPEQDIPQRLVKGLFQT
                     LFETGYFHGDLHPGNIMLTDEGNIAFVDFGIVGYLSFEKRKQMAGIISGVLQGKLPDT
                     LPHLKKFFGVSYHVPENFEKNVSFIVDKYGSLPLQKIHLADIVYDLMTLARKMSIRLD
                     SEIGLLARNLMSLEAICARINPAESLLNLSKSYWQPVLETGVFQRFWVEDIKNIFISY
                     RNLFAEFPETWEEFLELNRERVETEQKIVSRLDRYARSLEHAGTKIGFSIILLPFLTV
                     FLLIGIRSMHTGLLVLSVAIFILFAIFVFRFTAGRYDD"
     gene            complement(2314..>2569)
                     /locus_tag="PLW07_06370"
     CDS             complement(2314..>2569)
                     /locus_tag="PLW07_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50188.1"
                     /translation="LALGIASLSLAGKKGKEILESLEKEVKEKNLEEKIEKRFSQMLE
                     KMDRIKEIKKEKIAEVFGVATKEEIENLKKEIENLKHGNK"
BASE COUNT          727 a          609 c          404 g          829 t
ORIGIN      
        1 ccccagagag gccccttcaa taagccccgg aatatcagcg gcaacaaatt gcctttcatt
       61 gtccagaaca acaaccccga gatttggatt caatgtcgta aatggataat ctgcaatttt
      121 tggtctggca tttgaaattt tcgaaagaag cgtggatttg ccggcgttcg gagatccaat
      181 aatacccacc tctgcaatca gttttaattc aagtttgatg aatttttcct caccagatgc
      241 tccaggggtg gctattcttg gagtttgatt tgttgaactc ttgaaactcg cattgccctt
      301 tccccctttt ccacctctgg ctgcaagtac ctggttgccg tccctatcaa ggtctgccag
      361 cattacctct ttttctcctt ctctgatttc aataataacc gtaccaggcg gaactttaag
      421 aataatatct tttccatccg cgccggtacg attattacct tcacctggtt tgccactttc
      481 ggctttaaaa tgcctgtgat aataatattc tctcagtgtt ttcacattgc tacacgctct
      541 aaaatatacg gacccaccct tccctccatc ccctccagaa gggccacctt ttggcacaaa
      601 tttttctctt ctgaaagcaa tacaaccagg acctccatcc ccggctttta tccatattct
      661 cgcttcatca acaaaatcaa tcatcgtatc tcccagcagt gaatctaaaa acaaatatcg
      721 caaacagtat aaatattgca accgacagaa ccaatagtcc tgtgtgcata ctccgaatcc
      781 cgatcaacaa aaaaacagta agaaaaggta atagaataat agagaatcca atttttgtac
      841 ctgcatgttc cagactcctg gcatatctgt caagacgact gacaattttc tgttctgttt
      901 ccactctttc cctgttaagt tctaaaaatt cctcccaggt ttccggaaat tccgcaaaaa
      961 ggtttctata agagatgaat atatttttta tgtcctcaac ccagaaacgt tgaaaaactc
     1021 ctgtttcaag tactggttgc cagtacgact tgctgagatt taacaaactt tctgcaggat
     1081 tgatcctggc acagattgct tcaagactca tgagatttct tgcaagcagt cctatttcag
     1141 aatcaagccg tatactcatc ttcctcgcaa gagtcattaa atcataaaca atgtctgcca
     1201 gatgtatctt ttgcaacgga agagacccat atttgtccac aataaaactc acattttttt
     1261 caaagttttc tggtacatgg tatgacacac caaaaaactt ttttagatgt ggaagagtgt
     1321 ccggaagttt accttgaagc acaccagaaa tgatacctgc catttgtttc cttttttcaa
     1381 atgacagata tcctacaata ccgaagtcaa caaatgcaat gttcccttca tctgtcagca
     1441 tgatatttcc tggatgaagg tccccatgaa aataacctgt ttcaaaaagt gtttgaaaca
     1501 atcctttcac aagacgttgc ggaatatcct gttctggaag agaaacatca agaattttca
     1561 ctccgtgtat ctgttccatc gtaaggactc ttccagatgt gtattcccat aatacgtgag
     1621 ggaccctgat atccgttttc tcataaaaca cctttaattt ttccatcatt cctgcttcat
     1681 aaataaaatc catctgtctt cgcagtaatt ccccgacttc ttccagcaga tcggatatat
     1741 tccactgttt cagttcagga atatgctttg aagcaagccg tagtatttcc tttaacacat
     1801 caatgtcagc tgcaacaact tcttcaacac cgggtcttcg caccttaaca acacactcct
     1861 tgccggaaag aagtctggca aaatgcacct gtcctattga agcagaaaac tgtggttgtt
     1921 ctgaaaattc agcaaaaatt tcttccacct gtttcccgaa atcttcaaca atcgtttttt
     1981 tcactgcttc aaaaggaaaa ggagatacat tatcctgcag tttggataac tctttgagaa
     2041 aattttcagg tagaatatca gccctggtac tgaaaaattg tccaattttt atgtaggtag
     2101 gaccaagttc ttcaataaca caacgtaatc tttctgctgt acttaatgac tctttttttc
     2161 tggatgcaga tgctctatga aagcgaagaa ttctgaaatg tggttctcta aaaattctat
     2221 caataaaata accaaggccg tgtctgacta ttgttcgcaa tacgaaactt acacggcttg
     2281 caagccgata ttttttcctg actccaagta tgttcattta ttgccatgct tgagattttc
     2341 tatttccttc ttcaaatttt ctatttcttc ttttgtcgca acgccaaaaa cttcagcgat
     2401 tttctctttt tttatttcct ttatcctatc catcttttct aacatctgtg aaaatctctt
     2461 ctcgattttt tcttcaagat ttttttcttt cacttctttt tcaagactct ccagaatttc
     2521 ttttcccttt tttccggcaa gagaaaggga agcaatcccg agagcaagt
//
LOCUS       DAORYU010000213         2564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_27138_length_2564_cov_3.858908, whole genome shotgun sequence.
ACCESSION   DAORYU010000213 DAORYU010000000
VERSION     DAORYU010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27138_length_2564_cov_3.858908"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="PLW07_06375"
     CDS             <1..620
                     /locus_tag="PLW07_06375"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOL50189.1"
                     /translation="KRCSVAHLQKKQQLWGIIQGGTDINLRKICLENLENLSFPGYGI
                     GGLSIGEPWERALEILNDLCRIIPEEKVVYLMGVGMPDQIVESVDAGIDFFDCAMPTR
                     IARNGTTLSWSGKFNIKAAWCKNQQSPLDPECDCCVCRTYSRAYIRHLFNAKEMLGMR
                     LNSYHNLYFIGKLMNRIRQNIKQGTFQKFKKEFLGKYKRKEQYAQ"
     gene            610..903
                     /gene="yajC"
                     /locus_tag="PLW07_06380"
     CDS             610..903
                     /gene="yajC"
                     /locus_tag="PLW07_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOL50190.1"
                     /translation="MPNSAQGASLFNALLPLLLIFFIFYFLLILPQQRKEKQRREMLD
                     NLKKGDRVITIGGMIATVDEIKGKIVSLKIAQDIKVDFVKTAIAQIFQEEKKS"
     gene            900..2042
                     /locus_tag="PLW07_06385"
     CDS             900..2042
                     /locus_tag="PLW07_06385"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LarC family nickel insertion protein"
                     /protein_id="HOL50191.1"
                     /translation="MIGYFDIKNGCAGDMVCAALSGCVDVKEIERHLKKVKFPSRYEI
                     EIRQVMRAAGQFHGIKANQFIVNVSGKEHERSYREIVSIFSESDLLSSTKKKILKIFS
                     VLAEAESKVHGRDLEQLHFHAVGQTDALVEISFSVLAMEYIGITKLVVSSVGISNAAP
                     ATMEMISKMPVIFRNIPFEISTPTGIAIIKGLADFYGDSPTLMPLGYAYGTGTITTDL
                     PNTVEFVYGTEMSEKTETIGIIETSVDDMNPLIFEHLIDKLHQAGALEVCFFTGTTKK
                     SRPMFLIRVLCKPEQRATMSKIIFHETTSLGIRYREENRIVLGRAQKKIKTSYGEIRV
                     KFGYFAGETVNIMPEYEDCKKIAIEKRIPIKIVMQEAVKSAIESDR"
     gene            2098..2391
                     /locus_tag="PLW07_06390"
     CDS             2098..2391
                     /locus_tag="PLW07_06390"
                     /inference="COORDINATES: protein motif:HMM:NF016351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HOL50192.1"
                     /translation="MVVGVWMVELHIPYAQNLKDKRSVIQSILTKSRNRFNVSIAELG
                     YKDKWQRSIMGVACVDSESCRAENIFSAIRKIFEENGMVIVVKEDVNFYPLGE"
     gene            2397..>2564
                     /locus_tag="PLW07_06395"
     CDS             2397..>2564
                     /locus_tag="PLW07_06395"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOL50193.1"
                     /translation="MKDETRYNAEKIEHYWYSKWEESGCFNVTQQTGTPYVIVIPPPN
                     VTGYLHIGHALN"
BASE COUNT          851 a          379 c          569 g          765 t
ORIGIN      
        1 caaaaaggtg ttctgttgca caccttcaaa agaagcaaca actgtgggga attattcagg
       61 gcggtacaga tatcaatttg agaaaaattt gcctggagaa tctcgaaaat ttatctttcc
      121 ctggttatgg cataggcgga ttaagcattg gagaaccgtg ggaaagagca cttgaaattt
      181 tgaatgattt atgcagaata attccagaag agaaagttgt ttatctcatg ggcgttggca
      241 tgccagatca gattgtggaa tctgtggatg ctggtattga tttttttgat tgtgccatgc
      301 caacaagaat agcaagaaat ggaacgactc taagctggtc aggcaagttt aatatcaagg
      361 cagcatggtg taaaaaccag cagtcgccac ttgatcctga gtgtgattgt tgtgtatgta
      421 gaacatatag cagagcgtac atacggcatc tttttaatgc aaaggaaatg ctcgggatgc
      481 gacttaattc ttatcataat ttatatttta ttggaaaatt gatgaacaga attcgtcaga
      541 atatcaaaca gggaactttt cagaaattta agaaagagtt tttaggaaaa tacaaacgaa
      601 aggagcaata tgcccaatag tgcacaggga gcttcgttgt ttaatgcgct tttgcctttg
      661 ctgttaattt tctttatttt ttatttcctt ctgattctgc cgcagcaacg aaaggaaaag
      721 cagcgaagag aaatgctgga taatttaaaa aaaggtgatc gtgttatcac aattggcgga
      781 atgattgcta ctgttgatga aataaaggga aagattgtaa gtttgaaaat tgctcaggat
      841 ataaaggtgg attttgttaa aaccgctatt gcacaaatct ttcaggaaga aaaaaaatca
      901 tgataggtta ttttgacatc aagaatggtt gcgccggaga tatggtttgt gctgctcttt
      961 caggttgtgt ggatgtaaaa gaaattgaaa gacatctgaa gaaagtaaaa tttccctcaa
     1021 gatatgaaat cgaaatccga caggttatgc gggctgccgg gcaatttcat ggaatcaaag
     1081 ccaatcaatt cattgttaat gttagcggta aagaacacga aaggtcatac cgtgaaattg
     1141 tttcaatttt ttcagaaagt gacctgcttt cttcaacaaa gaagaaaatt ttgaagattt
     1201 tcagtgtcct tgcagaagca gaaagcaaag ttcacggcag agatcttgaa caattgcact
     1261 ttcatgcagt tggtcaaact gatgcgcttg ttgaaatatc tttttctgtc ctcgcgatgg
     1321 aatacatcgg aattacaaaa ttagttgttt cttctgttgg tatttcaaat gcagctcctg
     1381 caacgatgga aatgatttca aaaatgcctg taatatttcg taatattcct tttgaaatat
     1441 caacgcccac tggtatagca attattaaag gactggcaga tttttacggg gattctccaa
     1501 cgttaatgcc tcttggttac gcgtatggaa cagggacaat aacaactgat ttaccgaata
     1561 ccgtagagtt tgtgtatgga acagaaatgt cagaaaaaac agagaccata ggtattattg
     1621 aaacatctgt ggatgacatg aatccactga tttttgagca tttgatagat aaacttcatc
     1681 aagcaggtgc tcttgaggtc tgtttcttta ctggtaccac gaagaaatca aggcccatgt
     1741 ttttgataag ggttttgtgc aaacctgagc aaagagcaac aatgtcaaaa atcattttcc
     1801 atgaaacaac atctcttggt atcaggtaca gagaagagaa caggattgtt cttggtaggg
     1861 ctcaaaaaaa aataaagact tcttacggtg aaattagagt aaaatttggt tattttgctg
     1921 gtgaaacggt taacattatg ccagagtatg aagattgtaa aaaaattgct atcgaaaaga
     1981 gaatccccat aaaaattgta atgcaggaag ccgtgaaaag tgctattgaa tctgataggt
     2041 aaattaagat ataatatttc attaaaaatc ttcacttttt aataatcgga ggggaaaatg
     2101 gtggttggcg tatggatggt tgaattgcat ataccttatg cgcagaacct caaggataaa
     2161 agaagtgtaa ttcaatcgat tcttacgaaa tccagaaatc gttttaatgt atcaatagca
     2221 gaacttggtt ataaagataa atggcagaga agtattatgg gtgttgcatg tgttgattct
     2281 gaatcatgtc gggctgagaa tattttttct gctatccgta agatttttga agaaaatgga
     2341 atggttattg tggtgaagga agatgtaaat ttttatccac ttggggaata agtaatatga
     2401 aagatgaaac cagatataac gcggaaaaga ttgaacatta ctggtatagt aaatgggaag
     2461 aatcgggttg ctttaatgtg acacagcaga cgggtacgcc atatgtgatt gttattcccc
     2521 cacctaatgt cacgggatat cttcatatcg ggcatgcgct aaac
//
LOCUS       DAORYU010000214         2558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_27227_length_2558_cov_3.340392, whole genome shotgun sequence.
ACCESSION   DAORYU010000214 DAORYU010000000
VERSION     DAORYU010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27227_length_2558_cov_3.340392"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2195
                     /locus_tag="PLW07_06400"
     CDS             <1..2195
                     /locus_tag="PLW07_06400"
                     /inference="COORDINATES: protein motif:HMM:NF025038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOL50194.1"
                     /translation="IAKTKPTPDDWCYVYNFSAPDNPRCMRFPPGMAETFRKDMESLI
                     EELKIDLPKAFESKIYEEQKNTVIRKFQKRKEELITQLEEKARNASFALKHTPSGFVF
                     VPLIEGKPLTEEDVEKLTDEAKNEIKTKEEILVSELAEVLREIRQADRATREELQKIE
                     HETTMFTVSPRINELKEKYAEFPEVLKYLDEVQQDIIENPGNFEEKREIEFLPGLKMP
                     AKENQLFKYQVNVLIDNSKTEGAPVVKETQPTCYNLTGRIEYRPTLGAMFTDFTMIKP
                     GAIHKANGGFLILNILDVLRNYFAWEALKRTIKNREISIEDLNEQFRIINTPTLKPQP
                     IPANIKIILIGSPLLYHLLYAYDEDFEKLFKVRADFSSLMDRNIEGVRQYAKFISKRV
                     KEDKLKDFDRQAVAKVVEYGSRIVEDQKKLSTRFMEICDLLKEANHWASMDGSDIVKG
                     VHVQKAIEEKIYRSNLIEQRIEELIKEGVIIVDTDGEKIGQVNGLSVLSLGDYQFGKP
                     SRITVSVSMGKGGPVHIDREVKMTGSIHNKGFLILKGLFAEKFGKSYPLSFLATICFE
                     QVYEEIEGDSASSTEYYALISGISGIPLKQGIAVTGSINQKGEIQPIGGVNEKIEGFY
                     SVCKIKGLTGNQGVIIPKKNLTHLMLKEEVVEAIRHGRFHVWAVKDVDEGIEILTGIP
                     AGKPDGNGKYPEGTVNAAVAKKLMELAKKSKEIEKQLSPKTQKKNKNP"
BASE COUNT          929 a          455 c          532 g          642 t
ORIGIN      
        1 aaattgcaaa aacaaaacca acacctgatg actggtgtta tgtgtataat ttttctgccc
       61 ctgataatcc cagatgcatg agattcccac caggaatggc tgaaacattc agaaaggata
      121 tggaaagcct gattgaagaa ctcaaaatag atctgccaaa agcatttgaa agcaagatat
      181 atgaagaaca gaaaaataca gtgataagaa aatttcaaaa aagaaaagag gaactcatca
      241 cacaacttga agaaaaagca agaaatgctt cctttgctct caaacacaca ccttcgggtt
      301 ttgtttttgt tcccctgatt gaaggaaaac ccctcacaga agaagatgtg gaaaaactta
      361 ctgatgaagc aaagaatgaa atcaagacaa aagaagaaat ccttgtctca gaactggctg
      421 aagtactgcg agaaataaga caggcagata gggcaacgcg ggaagaactg cagaaaatag
      481 aacatgaaac taccatgttc acagtgagcc caagaatcaa tgaattaaaa gaaaagtacg
      541 cagaatttcc agaagtattg aaatatcttg acgaggtcca gcaagatatt attgaaaatc
      601 caggcaattt tgaagaaaaa agagagattg aatttcttcc cgggttaaag atgccggcga
      661 aagaaaatca acttttcaaa taccaggtga atgtgctcat cgataacagc aaaactgagg
      721 gagcacccgt tgtaaaggaa acacagccca catgctacaa cttaacagga aggattgagt
      781 acagacccac gcttggtgca atgttcactg actttaccat gataaaacca ggtgccattc
      841 ataaagccaa tgggggtttt ctaattttga acatccttga tgtactgcgt aattactttg
      901 cgtgggaagc attgaaaaga acaataaaaa acagagaaat cagcatagaa gatttgaacg
      961 aacagttcag aatcatcaac actccaacat taaaaccaca gccaattcca gcaaatatca
     1021 aaattattct tattggaagt ccacttcttt atcatcttct gtatgcatat gacgaggact
     1081 ttgaaaaact ttttaaggtg agggctgatt tcagttcttt aatggacagg aacattgagg
     1141 gcgtcaggca gtacgcaaaa tttatcagta aacgggtcaa agaagacaaa ctcaaggact
     1201 ttgacagaca ggcagttgca aaggttgttg aatatggctc aagaattgtt gaagaccaga
     1261 aaaaactttc gacaagattt atggaaatat gtgatttatt gaaggaagca aatcactggg
     1321 caagtatgga tggttctgac atagtaaaag gagttcatgt tcaaaaagcc atagaagaaa
     1381 aaatatacag atccaatctt attgaacaga ggattgaaga actgattaag gaaggtgtca
     1441 ttatcgttga tactgatgga gaaaaaattg gtcaggtcaa tggactcagt gttttgagtt
     1501 taggggacta tcaattcggg aaaccatcgc gaattactgt aagtgtttcc atgggaaaag
     1561 gtggaccagt gcatattgac cgtgaagtaa aaatgactgg cagcatacac aacaagggtt
     1621 ttttgatttt gaaaggactt tttgctgaga aatttggaaa aagctacccg ctttcatttc
     1681 tggcaactat ttgctttgaa caggtttatg aagaaataga aggagatagc gcttcatcaa
     1741 ctgaatacta tgccttaata tcagggatct caggaatacc tttaaaacag ggaatagcag
     1801 ttacgggctc catcaaccag aaaggagaaa ttcagccaat tggcggagtg aacgaaaaaa
     1861 ttgaaggttt ttattcggtg tgcaaaatca agggattaac aggaaatcag ggtgtcatca
     1921 ttcctaaaaa gaatcttacc catttaatgt taaaggaaga agttgtcgaa gcaatcaggc
     1981 atgggagatt ccatgtctgg gcagtaaagg atgttgatga aggaatcgaa atacttactg
     2041 gaattccagc aggaaagcca gacggaaacg gtaaatatcc agaaggaaca gtgaatgctg
     2101 ctgttgcgaa aaaattgatg gaactggcaa agaagtcaaa ggaaatcgaa aagcaacttt
     2161 caccaaaaac gcagaaaaaa aacaaaaatc cctgagatct tttccttgtg tttcaacaac
     2221 ttctcgtatc ctcgaaaatt tcctgttacc cgcttttttg aaattttaaa tcccacccct
     2281 ctaaatgaat gtccctggtc acttccctat tataaacgtt ccaccccact ttttataaac
     2341 tctcctaata tcccctcttt atatattccc tcaacatctc ccctctttat aaaagagggg
     2401 ctgggggaga tttcttaaaa tttccctgca actttaggtc cccttccttt acaaaaggaa
     2461 ggctgggata aatttctgaa atccccctta atcccccttt tgtaaagggg gaaaacaaaa
     2521 ggaggaattt gaaaatgggg gaattgaggg aggaattt
//
LOCUS       DAORYU010000215         2550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_27333_length_2550_cov_4.345491, whole genome shotgun sequence.
ACCESSION   DAORYU010000215 DAORYU010000000
VERSION     DAORYU010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27333_length_2550_cov_4.345491"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="PLW07_06405"
     CDS             <1..640
                     /locus_tag="PLW07_06405"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOL50195.1"
                     /translation="YPVGVSAGSFFGWIRTWIGIENMSYLIVDDPGLIEDMEEHLEYL
                     FLSVLERVLKDVKVDFAHFWEDMAYKTGPLVSPSFVKKFMVPHYKKITEFLRSKGVDI
                     ITLDSDGNIWELIPIWLECGINGVLPNEVAAGMDVVEMRKKFGKNLIICGGIDKRVLL
                     KSKKDIEEEVKRKVGTLLEDGGYFPVIDHAVPPDVPFKNFMYYLEILKKSAT"
     gene            686..1783
                     /locus_tag="PLW07_06410"
     CDS             686..1783
                     /locus_tag="PLW07_06410"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOL50196.1"
                     /translation="MKKVPLSKPEPDFKRFVDVIKGNVIPKIPPLIELFLDFEIVREI
                     SKNVLGREWVIGEDIESRKKYFLNSIEVYWRMGYDCYRISGAIGLEFTGKGRPGKDTA
                     PLSRGTRNWAEEGKGPISTWEDFETYPWPDPRKFDYWYYDFISKNLPDGMGILVCPSS
                     GFLEIPLDTLFGYENLCYLMYDNPELVTAVFNRVGEILFSYYEHIVSLPKLVGFFQGD
                     DMGFKTGTLVPPAFLRTHVLPWHKKLAKLAHDNGLIYLLHACGNLEQIMPDLIDDVKI
                     DGKHSYEDEGNSVVDAKRKYGDKIAILGGIDVDKLVRMEENQLRQHVRNILDVCMPGG
                     RYCLGSGNSVTNYVPVENYFAMVEEGLNYKP"
     gene            complement(1785..>2550)
                     /locus_tag="PLW07_06415"
     CDS             complement(1785..>2550)
                     /locus_tag="PLW07_06415"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOL50197.1"
                     /translation="AFLRFLSASIFLALYHFAKGERFFLKSSDIIHFLILGASGIFGM
                     NAFIFYSLTLTDSTITSLLMNANGFIIGILGFFLLKERISMLEMMGLIVGTGGCYLIF
                     TQGDISRISHSNLAGNTLAALASFCWAFYSVWSKKTGVIEKYGAVLSTLWACVFGTVM
                     LGIAVLMLSVPLHFDMKNVLIGVYLGIVPAGVGFTLWFYSISKLKTIIPGIIQFIAPL
                     TTAVLAVILLRQNISVATVIGGLLIIAGVLFTLEKQ"
BASE COUNT          853 a          453 c          544 g          700 t
ORIGIN      
        1 atatcctgtt ggagtgtcag caggaagttt ttttggctgg attagaacct ggataggcat
       61 agagaatatg agttacctta ttgttgatga ccctggtctg atagaggaca tggaggaaca
      121 tctggaatat ctgtttcttt ctgttctgga aagggtattg aaggatgtta aggtggattt
      181 tgcgcatttc tgggaggaca tggcatacaa aaccggacca ctggtttcac cttcctttgt
      241 aaaaaaattt atggtccccc attataagaa gataacagag tttcttcgtt caaaaggagt
      301 ggatatcatc acccttgatt cagatggaaa catctgggaa cttattccta tatggcttga
      361 atgcggaatc aatggagtcc ttccaaatga ggttgcagca ggaatggatg tggtagaaat
      421 gagaaaaaag tttgggaaaa acctcattat atgtggtggt attgataaaa gggtgcttct
      481 gaaatcaaaa aaggacatag aagaagaggt gaaaagaaaa gttggaacac ttctggaaga
      541 tggtggatat ttccctgtca ttgaccatgc tgttccacca gatgtgccgt ttaagaattt
      601 tatgtattac cttgaaattt taaaaaaatc agcaacatga taaaatttca tgtgatgtcg
      661 ccagaatcaa acaaaaagga gacacatgaa aaaagtacct ctatccaagc cagaaccaga
      721 ttttaaaaga tttgtagatg taataaaggg caatgtgatt cctaaaatac cacctcttat
      781 tgaattgttt cttgactttg aaattgtaag agaaatatca aaaaatgtgc tggggagaga
      841 atgggtcata ggtgaagaca ttgaatccag gaaaaagtac tttctcaatt caattgaggt
      901 gtactggaga atggggtatg actgctacag aatctctggt gctattggcc ttgaatttac
      961 gggcaaggga agacctggaa aagacactgc accactatca agaggaacaa ggaattgggc
     1021 agaagaagga aagggtccta tttcaacctg ggaagatttt gaaacatacc cctggcctga
     1081 cccgcgaaaa tttgattact ggtactatga tttcatttca aaaaatctcc ctgatggaat
     1141 gggaattctg gtctgtccat caagtggttt tcttgaaatt ccccttgata ctctttttgg
     1201 ctatgaaaat ctgtgttatc tcatgtacga taatccagaa cttgtgactg ctgtcttcaa
     1261 cagggtgggt gaaattcttt tttcttacta tgaacacatt gtatcccttc cgaaactggt
     1321 aggatttttc cagggcgatg acatgggatt taaaacagga acacttgttc cacctgcttt
     1381 tttaagaaca catgtgttgc cctggcacaa aaaacttgca aaacttgccc atgataatgg
     1441 gttgatttat cttctgcatg cctgcggaaa tctggagcag attatgcctg atttgattga
     1501 tgatgtgaaa attgatggaa agcattccta tgaagatgag ggaaattcag ttgttgacgc
     1561 aaaacgaaaa tatggtgata aaattgcaat tttaggcggt attgatgttg ataaactggt
     1621 aaggatggaa gagaatcaac tgcggcaaca tgtaagaaac attcttgatg tgtgcatgcc
     1681 aggtggtaga tactgccttg gttcaggaaa ttctgtgaca aattatgtgc ctgtggaaaa
     1741 ttactttgca atggtggaag agggtctaaa ttacaagccc tgaactattg tttttccagg
     1801 gtaaaaagaa ctccagcaat aatgagcaat ccgccaataa ccgtagcaac gctgatgttt
     1861 tgccttaaaa gaattacagc aagcactgct gtggtcaacg gagcaataaa ctgaataatt
     1921 ccaggaataa ttgttttcag tttgctgatg gaataaaacc ataacgtaaa accaactcca
     1981 gcaggaacaa ttccaagata cacgccgata agaacatttt tcatgtcaaa atgtaaggga
     2041 acactcaaca tcagcaccgc aataccaagc ataacagtcc caaacacaca agcccacaag
     2101 gtacttaata ctgccccata tttttcaatc acacctgttt tcttgctcca gacagagtaa
     2161 aaagcccagc agaatgaagc aagcgctgca agtgtatttc cagcaagatt tgagtgagaa
     2221 atcctgctga tatcaccctg agtaaaaatt aaataacaac cgcctgtacc cacgataagc
     2281 cccatcattt caagcataga aatcctttct ttcagcagga aaaatcccag tattccaata
     2341 ataaatccat tggcattcat caacaaactg gtgattgtag aatcagtcag tgtcagggag
     2401 taaaaaataa aagcattcat tccaaaaata ccgctggctc caagaataag aaagtggatg
     2461 atatcactgg atttaagaaa aaacctttct ccttttgcaa aatggtacaa agcaagaaaa
     2521 atactggctg aaagaaacct caaaaatgct
//
LOCUS       DAORYU010000216         2517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_27801_length_2517_cov_5.281072, whole genome shotgun sequence.
ACCESSION   DAORYU010000216 DAORYU010000000
VERSION     DAORYU010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27801_length_2517_cov_5.281072"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /gene="murB"
                     /locus_tag="PLW07_06420"
     CDS             complement(<1..859)
                     /gene="murB"
                     /locus_tag="PLW07_06420"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOL50198.1"
                     /translation="MKNVFVDKKILVNIFGDRISFDVPAKCFTSFKTGGILPVVVFPL
                     NKQEIEFLYSLKQAGIAVKFLGAGSNILVSDEGFDGIIASTKNLSGIEELEGFLNVLA
                     GTRVNSLIGYCVKNNLAGLEFLTGIPGTIGGCLIMNAGTKQKSISDAVKIVEYMNHEG
                     IWLKENKKNLLWKYRHSQLKEKSFFIFSCSLKTEAGNRETIRRIIADTMQKRKTSQPL
                     EYPSAGSVFKNPPDGFAGKLIEDAGLKGYRIGDAAVSEKHANFIINLGSATSSDIWKI
                     ICHIQETVKK"
     gene            complement(867..1622)
                     /gene="truA"
                     /locus_tag="PLW07_06425"
     CDS             complement(867..1622)
                     /gene="truA"
                     /locus_tag="PLW07_06425"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOL50199.1"
                     /translation="MKQVRFKVKVTYDGSDFAGWQVQPDKKTVQLELEKVLSQIFQQD
                     VKIQGCGRTDTGVHATGYVFSFSVRTRMSPVQVMKATNALIDKQIRIKEISYAETDFH
                     PRFEAQKKVYRYLITDFNSPFLTNRAWYVCQKLDVTKMKKAASCIVGTHDFACFQASG
                     RKAKNTVRTIEYIKIKREKFSLDPDIKLLVIEICGTGFLYKMARNIVGTLVDVGKNKR
                     LPEHIKGIVESKDRRLASATAPGYGLYLKEVIY"
     gene            complement(1619..2383)
                     /locus_tag="PLW07_06430"
     CDS             complement(1619..2383)
                     /locus_tag="PLW07_06430"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOL50200.1"
                     /translation="MEKIGAEYFNKFLLYLPKIPLALIIVFAGWLFGRTWNRFYLRIS
                     RNSRIDALARKYIGRLVYMAIFAFSIIIALSTLGLNISPFLAGIGAGGLIIGFGVRET
                     VADFAAGIMIFIYRPFKIGDLVKIGGVEGEVITISPVNIELRAKDSRKILVPNRSAWG
                     QIIYNATKDGKNIIALQMTVNADTSNVEEIVRPVFQQFNVKHRCCLTGVIASGLNYTI
                     YMELPKEMCVEVDQIVRNLWLQMKEKNINATITLQS"
BASE COUNT          751 a          546 c          385 g          835 t
ORIGIN      
        1 gttttttcac ggtttcctga atgtgacaga ttattttcca gatatcagaa gatgtagcgc
       61 ttccaaggtt aataataaaa ttcgcatgct tttctgaaac agcagcatcc ccaattctat
      121 agcccttcaa tcccgcatcc tcaatcaact ttcctgcaaa accgtccggc ggattcttaa
      181 acacagatcc agcagacgga tattccagcg gctggcttgt ttttcttttt tgcatggtgt
      241 cggctatgat gcgccttatt gtttctctat tgcctgcttc tgttttcaaa ctgcatgaaa
      301 aaataaaaaa agatttttct tttagttgcg aatgcctgta tttccataaa agattttttt
      361 tgttttcttt aagccatatg ccttcatgat tcatatactc aacaattttg acagcatctg
      421 aaatactttt ctgttttgtt cctgcattca taattaggca accaccaatt gtacctggaa
      481 tgcctgtaag aaattcaaga cctgcaagat tattttttac gcagtatcca atcaaactgt
      541 tcacccttgt tcctgcaaga acattcaaaa aaccttctag ttcttcaata ccagaaagat
      601 tttttgtgct tgcgataata ccatcaaatc cctcatcgct cacaagaata tttgaacctg
      661 cacccaggaa tttcaccgca attcctgctt gtttcaaact gtacagaaat tcaatttcct
      721 gcttgtttaa aggaaaaacc acaacaggta agattccgcc tgttttaaaa cttgtaaaac
      781 attttgcagg aacatcaaat gaaatacgat ccccgaaaat gttcacaaga atctttttat
      841 caacaaaaac atttttcatg gttttgttag taaatcactt ctttcaaata caaaccatat
      901 cccggagccg ttgctgaagc aagcctcctg tcttttgact caacaatacc ttttatatgt
      961 tctggcaatc ttttattttt gcccacgtct acaagggttc caacaatgtt tcgcgccatt
     1021 ttataaagaa atcctgttcc gcatatctca atcacaagca gtttgatatc agggtcaagg
     1081 gaaaatttct cacgttttat tttaatatac tcaatagttc taaccgtatt ttttgccttt
     1141 cttcctgatg cctgaaaaca tgcaaaatca tgtgttccaa caatacatga agctgctttt
     1201 ttcatttttg taacatcaag tttctgacag acataccatg ccctgtttgt taaaaaaggc
     1261 gaattgaaat ctgttatcaa ataacgatac actttttttt gcgcctcaaa tcttggatga
     1321 aaatctgttt ctgcgtaaga aatctctttt attcttattt gtttatcaat gagggcgttt
     1381 gtcgctttca ttacctgcac cggactcatc cttgttcgaa cagaaaaaga aaacacataa
     1441 cctgttgcgt gaacgccagt atcagtccta ccacatccct gaatttttac atcctgttga
     1501 aaaatttgtg aaagaacttt ttctaattct aactgcacag tttttttatc tggctggacc
     1561 tgccatccgg caaaatcact accatcgtat gtaactttta ccttaaatct gacctgtttc
     1621 acgattgaag ggtaattgtg gcattgatat ttttctcttt catttgcagc cagagatttc
     1681 taacaatctg atccacttca acacacattt cttttggaag ttccatgtat attgtgtaat
     1741 tcaaaccaga tgctatcaca ccagtcaaac aacatctgtg ttttacattg aattgctgaa
     1801 aaacaggtct tacaatttct tcgacatttg atgtgtctgc gtttacagtc atttgaagcg
     1861 caatgatatt tttcccatcc tttgttgcat tgtaaataat ctgcccccat gcactgcgat
     1921 ttggaacaag aatttttcta ctatcttttg ccctgagttc aatattgaca ggactgattg
     1981 tgataacctc tccctcaacc cctccgattt ttacgaggtc tcctattttg aaaggtctgt
     2041 atatgaaaat cataattcct gctgcaaaat cggcaacagt ttctcttaca ccaaaaccaa
     2101 taatcaatcc accagcacct attcctgcaa gaaagggaga gatattcaac cccagcgttg
     2161 aaagagcgat aataattgaa aaagcaaata tcgccatata gacgagccga cctatgtatt
     2221 ttcttgccag ggcatctatt ctggaatttc ttgatattct tagataaaac cgattccagg
     2281 tccttccaaa aagccaccct gcaaacacaa tgattaacgc aagcggaatt ttcggcaaat
     2341 acagcagaaa tttattgaaa tattcagcgc caattttttc catagattaa ataataaaac
     2401 caatggcagg taaagtcaat ttatctatat agcatatgtc aatcaaatga catgttataa
     2461 tatattgcgg atacactgga gggttagcct aacggtaagg cagtggactt gaaatcc
//
LOCUS       DAORYU010000217         2513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_27858_length_2513_cov_2.729048, whole genome shotgun sequence.
ACCESSION   DAORYU010000217 DAORYU010000000
VERSION     DAORYU010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27858_length_2513_cov_2.729048"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="PLW07_06435"
     CDS             <1..691
                     /locus_tag="PLW07_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOL50201.1"
                     /translation="ASFRALNLFLKTWSQQVLKWDKTEHKEGVGIHPVFKTEITAAEN
                     QISCGEFLKIVQKGSIQEIIEASDKVKFLCSAQESSEMQEALKILIKSEDIRLRLLGP
                     KFLARFQDRQLLVFVMNLLYDKEWIVRANAARAVIQTPFLLEVIKDVFSKDDRYAKDI
                     IIKTLEQNIWAFDNLIKTLNEKEMLEIDNTILKNSDLLSNTYNQLMSSKANADNKKGI
                     EIVFPDDVSSV"
     gene            complement(623..1345)
                     /locus_tag="PLW07_06440"
     CDS             complement(623..1345)
                     /locus_tag="PLW07_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50202.1"
                     /translation="MKKNILSGIFLFLFIACVQKLQAGDFFTEFYSEAVKANLTGLST
                     SAKLKIGIKEQKVDYYLYLFYEWADTTGTDLPFEYRYQGLTAGVEIRYWLIERSLAAI
                     LGYGFGLTDNVDGKNNFQYGLIGYHAFEKEKLFRDLYGELLWIGRADDVFMTLRYRHG
                     KTLYKTPDTRLWAYGVIFLNGSGKGKNGTENRIEAGPGIGYIFLNGKASANLELKYGH
                     SYRGNIVRENYFNPFFVISVGF"
     gene            complement(1342..2430)
                     /gene="wecB"
                     /locus_tag="PLW07_06445"
     CDS             complement(1342..2430)
                     /gene="wecB"
                     /locus_tag="PLW07_06445"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOL50203.1"
                     /translation="MHTAIIIGTRPEIIKTSPLIKKLHHLKFPFLVIHTGQHYSENMD
                     AVFFTQLNLPQPDIRINVGSGSHGEQTGRCLEALERILTKRQPEVILVQGDTNTVLAG
                     ALAASKLNIKIGHIEAGLRSYDRSMPEEINRVIADHLSYYLFAPTKLSKDNLYKEGIT
                     KNVFVTGNTIVEALKENIKMAEEQINDEIFKRFKIEKGKFFLMTLHRQENVDNKQKLE
                     QIILGINDVVKKFNMPVIFPVHPRTVKNFKRFKFNKILEKITLTEPLGYFEFLKLQKY
                     ACLILTDSGGLQEESCILRIPCVTLRENTERPETLNVGSNLLAGWQREKILSSVCKMI
                     NLEKKWKHPFGNGKTSDRIINIITKEKI"
BASE COUNT          797 a          454 c          417 g          845 t
ORIGIN      
        1 cgcttctttc agggctctga atttgttttt aaaaacctgg tcacaacagg ttctgaaatg
       61 ggataaaact gaacataaag aaggagttgg aatacatcct gtttttaaaa cagaaattac
      121 agcagcagaa aatcaaattt cctgtggtga gtttttaaaa attgtacaaa agggttcaat
      181 ccaggaaatt attgaagcat ctgataaagt gaaattcttg tgttcggctc aagaatcttc
      241 agaaatgcag gaagcactga aaatcttaat aaaatcagaa gatattcgtt tgagattgct
      301 cggtccaaaa tttcttgcaa ggtttcaaga tcgacaattg ttagtttttg taatgaacct
      361 gctatatgat aaagagtgga ttgtaagggc aaatgctgca agggcagtca ttcaaacacc
      421 gtttcttctt gaagtgataa aagacgtttt ttccaaagat gatagatatg caaaagatat
      481 cattataaaa acactggaac aaaacatctg ggcattcgac aatttaatta aaacactgaa
      541 cgaaaaggaa atgctggaaa tagacaatac aattttgaaa aattcagatt tactttcaaa
      601 cacttataac cagttgatgt cttcaaaagc caacgctgat aacaaaaaag ggattgaaat
      661 agttttccct gacgatgttt cctctgtatg agtgcccata ctttagttca agatttgctg
      721 atgctttgcc attcaggaaa atatatccaa ttcctggacc tgcttcaatc cgattctctg
      781 taccgttttt gcccttccca gacccattga gaaatattac tccatacgcc cacagacgcg
      841 tgtcaggtgt tttatacaaa gttttgccat gcctgtatcg cagagtcatg aagacgtcat
      901 cagctctgcc aatccataaa agttcgccat aaagatctct aaacaatttt tctttttcaa
      961 aagcatggta acctataaga ccgtattgaa aattattttt tccatcaaca ttgtcggtta
     1021 atccaaaacc ataacctaaa atagcagcca gacttcgttc aatcagccag tatcgtattt
     1081 caacgcctgc tgttaatcct tggtatctgt actcaaaagg aaggtcagtt cctgtggtat
     1141 ctgcccactc ataaaaaaga tacagataat aatcaacctt ttgttctttt atacctatct
     1201 tcaattttgc tgatgtagat aatcctgtaa ggtttgcctt tactgcttca gaataaaatt
     1261 ctgtaaaaaa atcacctgcc tgcaattttt gaacacaagc aataaacaga aaaagaaata
     1321 tgcctgatag tatgtttttt ttcatatttt ttcctttgtg atgatgttaa tgattctgtc
     1381 acttgtttta ccatttccaa agggatgttt ccattttttt tcaagattga tcattttgca
     1441 aacggatgaa agtatctttt ctctctgcca tccagccagc aagttactac caacatttag
     1501 ggtttctggt ctttcggtgt tttctctcag cgtaacacag ggaattctaa gaatacatga
     1561 ctcttcctgt aaaccaccag aatcagtgag aattaaacat gcatattttt gcaattttaa
     1621 aaattcaaaa tatccaagtg gttctgtaag tgtaattttc tccagaatct tattgaattt
     1681 gaatctctta aaattcttta ctgttcttgg atgaaccgga aagataacag gcatgttgaa
     1741 tttcttcaca acgtcattga taccaagaat gatttgttcc agtttttgtt tgttatcaac
     1801 attttcctgg cgatgtaagg tcataagaaa aaacttgcct ttttctattt taaacctttt
     1861 aaatatttca tcattgattt gctcttcagc catttttata ttttctttca atgcctctac
     1921 aatggtatta ccggtgacaa aaacgttctt tgtgatacct tctttgtaaa gattatcctt
     1981 gcttaatttt gttggagcaa aaagataata tgaaagatgg tctgcaatta ccctgttgat
     2041 ttcctctggc attgaacggt cataactgcg tagcccggct tcaatatgac caattttaat
     2101 attgagtttt gatgctgcaa gagcaccagc aagcacagta ttggtatctc cttgaactaa
     2161 aatcacttca ggttgtcttt ttgttaaaat tctctcaagg gcttcaagac atctacctgt
     2221 ttgttctcca tgagaacctg aaccaacatt gattctgata tccggctgcg gaagattaag
     2281 ttgtgtaaag aaaaccgcat ccatgttttc agaataatgc tgaccggtat ggattactaa
     2341 aaagggaaat ttcaaatgat gaagtttttt tattaatggc gaagttttta ttatttcagg
     2401 cctcgtacca attatgattg cagtatgaat cttttctcat actaatttat tttaacacaa
     2461 atttctgttt ttgaaaaaat gctgatttag tataaaataa aacagaacca agg
//
LOCUS       DAORYU010000218         2483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_28277_length_2483_cov_3.768946, whole genome shotgun sequence.
ACCESSION   DAORYU010000218 DAORYU010000000
VERSION     DAORYU010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28277_length_2483_cov_3.768946"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            50..367
                     /gene="nrdD"
                     /locus_tag="PLW07_06450"
     CDS             50..367
                     /gene="nrdD"
                     /locus_tag="PLW07_06450"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: protein motif:HMM:NF024984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HOL50204.1"
                     /translation="MERSKFIEHLKKYPHLYEFEEKKDNGYSGILVKDKEYSTLTFFT
                     DEAIREGELVSLLKQTHHGKNVEWISRVTGYFSKINSWNKGKREEFKDRYRASNIGAG
                     VTE"
     gene            complement(357..1505)
                     /locus_tag="PLW07_06455"
     CDS             complement(357..1505)
                     /locus_tag="PLW07_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOL50205.1"
                     /translation="MTQIFILGLVSFFVDISTEMVYPLLPIFVTGVLGASVAVLGIIE
                     GLAESIASLLKVFSGYISDRVQKRKFLAIFGYGFSWAGKIFFALATSWQHVFTGRVLD
                     RIGKGIRTAPRDALIADYSDKSKRGYAYGIHRTLDTLGAFAGVGIVLLIVSKTSFDFH
                     NAQIYRNIFLLSIIPAITGWLILFAIKDKPVLSSSRQKISLSFRNLPGKLKLFLVFTF
                     VFALGNSSNQFLLLKIQRDTGSLVTTLQAYLIFNLVYAALSIPCGKISDVIGQKTILV
                     TGYFVYGIVYWLFAGHGNISFYFSALGLYGLYMALTEGVEKSLVSQLAPADMRASLLG
                     LHATLVGIGLLPASIITGVLWQKFGSFVAFGFGGSLGIIASIGLMIIL"
     gene            complement(1502..>2483)
                     /locus_tag="PLW07_06460"
     CDS             complement(1502..>2483)
                     /locus_tag="PLW07_06460"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOL50206.1"
                     /translation="SLLGISDGVVFSHDKSTGNINILVNIEGSSINKEMISAALKTIQ
                     NDRVRIFNRHLPEELRRHGLSNMLVLQILEEPMMNIYGIFVNKKPGPVSSAVAFTKND
                     YQWIASFCLQIVVAVFNTQLNDALKKSQLETIMRLATAIEYRDRETGMHIARVSEYCA
                     LIATEINLPKIEVELIKSAVPLHDLGKIAIPDSVLLKPSALTDEEKEIIKSHTIIAAK
                     MLEGSDSFILQAAYLIALYHHEKFDGTGYPYGLKGNAIPLYGRIASLADVFDALSSKR
                     VYKEAVSFESSIQKIIELAGKDFDPQIVDAFVKNKELAFEIYTKYQENLQ"
BASE COUNT          830 a          509 c          466 g          678 t
ORIGIN      
        1 atgcgcaaaa aatcggttga acattttgca aacctaaaca ggaggatgaa tggaaagaag
       61 taaatttata gaacatttga aaaagtatcc tcatctttat gagtttgagg aaaaaaagga
      121 caatggttat tctggtatac ttgttaagga taaagaatat agtaccctga cattctttac
      181 tgatgaagca atcagagaag gagaactggt gagcttgctt aaacagacac atcacgggaa
      241 aaatgttgaa tggatcagca gggtaacagg atatttttca aagattaatt catggaataa
      301 ggggaaacgg gaagaattta aggacagata cagagcgagc aatatcggtg ctggtgttac
      361 agaataatca taagtccgat agatgcaata attccaaggc ttccgccaaa gccaaaagca
      421 acaaaagaac cgaatttctg ccacaaaacg cctgttatga tagacgccgg cagtaatcca
      481 attcccacaa gtgttgcatg tagccccaat aaggaagccc tcatatcagc aggagcaagc
      541 tgactcacca gggatttttc aacgccctca gtcaaagcca tatacaatcc atacaaacca
      601 agggcagaaa aataaaaaga aatattaccg tgtccagcga aaagccagta aacaatgcca
      661 tacacaaaat atcccgtaac aagaattgtt ttctgtccta ttacatcaga aattttaccg
      721 catggaatag acagagcagc atacaccaga ttaaaaataa gatatgcttg cagggtggta
      781 accaatgaac cagtatctct ttgaatttta agcagcagaa actgatttga agaattgcca
      841 agggcaaaaa caaaggtgaa aacaagaaac aactttaatt taccgggaag attcctgaaa
      901 gacagagaaa ttttctgcct gcttgatgat agaaccggtt tatccttgat tgcaaaaagt
      961 atcaaccatc cagtaattgc gggtatgatg gaaagcaaga aaatattcct gtaaatttgc
     1021 gcattgtgaa aatcaaaaga agtttttgaa acaatcagca aaacaatgcc gacgccagca
     1081 aatgcaccaa gtgtgtcaag ggttctgtgt attccatagg cataccctct cttgctctta
     1141 tctgaataat cagcgatgag cgcgtccctt ggcgctgttc ttataccctt gcctatcctg
     1201 tcaagaaccc tgcctgtaaa aacatgttgc catgatgtgg caagcgcaaa aaatatcttg
     1261 cctgcccatg aaaagccata accgaaaatc gcaagaaact ttcttttttg aaccctgtct
     1321 gaaatatacc ctgaaaaaac cttcaaaaga cttgcaatac tctctgccag tccttctatg
     1381 attccgagta ctgcaacgct tgcaccgagt acccctgtta caaatatcgg gagcaaagga
     1441 tacaccatct cagtgcttat atcaacaaaa aaactgacca ggccgaggat gaaaatctga
     1501 gtcattggag attttcttga tattttgtat aaatctcaaa tgcgagttct ttgtttttca
     1561 caaacgcgtc aactatttga gggtcaaagt ccttccctgc aagttcaata attttctgta
     1621 tggatgattc aaaagaaacc gcttctttat ataccctttt tgaactgagg gcatcaaaga
     1681 catctgcaag cgacgcaata cgaccataaa gaggaattgc attacccttc aatccatatg
     1741 gataacccgt cccgtcaaac ttttcatgat gatacagtgc aatcagatat gccgcctgaa
     1801 gaataaaaga atcagaacct tcaagcatct ttgcagctat gatggtatgg cttttaatga
     1861 tttctttttc ttcatctgtt aatgctgatg gtttcaacag tacactatcg ggtatggcaa
     1921 ttttacctaa atcatgaagt ggtacggctg attttatcaa ttcaacctcg attttaggaa
     1981 gattaatctc tgtggctatc aatgcacaat actcgcttac ccgcgcaata tgcatacctg
     2041 tttcacggtc tctgtattca atggcagttg ccaatctcat aattgtctca agctgggatt
     2101 ttttaagagc atcgttcagt tgtgtgttaa acaccgcaac cacaatttga agacagaatg
     2161 atgcaatcca ttgatagtca ttttttgtaa aagccacggc tgaagacaca ggtcctggtt
     2221 tcttattcac aaaaataccg tatatattca tcattggttc ctcaagaatc tgcaaaacta
     2281 acatatttga aagaccatgt cttctcaatt cttctggaag gtgcctgttg aatattctga
     2341 ccctgtcgtt ttgaatcgtt ttcaacgcag cagaaatcat ttctttgtta atactggatc
     2401 cctctatatt gacaagaata ttgatatttc cggtgctctt atcatggctg aacacaacac
     2461 cgtcagaaat cccgagcaaa gaa
//
LOCUS       DAORYU010000219         2480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_28319_length_2480_cov_2.746392, whole genome shotgun sequence.
ACCESSION   DAORYU010000219 DAORYU010000000
VERSION     DAORYU010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28319_length_2480_cov_2.746392"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="PLW07_06465"
     CDS             complement(<1..533)
                     /locus_tag="PLW07_06465"
                     /inference="COORDINATES: protein motif:HMM:NF015891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOL50207.1"
                     /translation="MKTLFLKIIVLYIFILSLNCIASQKIESLTFNQTKLSLVLEILS
                     KKTGMKLISDTDISDKSITAYLENISPEQAIESILAANGLYREKLPDSDIYIVYSEQR
                     KISSQKPVSACFQLKHAFASDIATELKNIGFEKSIAVDNRTNVLVIKDTPANIEIVKA
                     IIENLDIEHKNLPVKTTV"
     gene            complement(548..1609)
                     /locus_tag="PLW07_06470"
     CDS             complement(548..1609)
                     /locus_tag="PLW07_06470"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOL50208.1"
                     /translation="MDKKNKKSLILLLMLLVIFIFTLTRALKPVKPKTIVMSSPDGKV
                     EKINIQENEKSSMEDKVFSFQEIDQSVNKLQSLMKSIEEEEQQVASVSVSKDPFTKPG
                     TKQLSPGTGQSPYTSILVQEIPAPDFKISGIVYDKEKPMVILDDEIKSENETKSGYLV
                     KKILPDRVILKYKDKDFILYVKSDSIESTGISEIKLSDAIETGDTDIATSTANTYRSI
                     LKDNSPKFATVEKTDTVSSQPVVRQKEITRSQSIVLPDVNENAQKILTIQVASFAGNK
                     KTQAIEFAKMLLSGGYENVRVEKIKNMYTVRIGVASDADILVPLCDGLRKYSETSFVR
                     TAFYIQERIIFPPENDTKI"
     gene            complement(1613..2140)
                     /gene="pilO"
                     /locus_tag="PLW07_06475"
     CDS             complement(1613..2140)
                     /gene="pilO"
                     /locus_tag="PLW07_06475"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HOL50209.1"
                     /translation="MKNHASSIKTHLVIMLILYGGTWLIINNVVIKQQHRILQQYRDE
                     KESLEYDYMKLKNYPDHINTIRKTIDNAGNKLDRFLWINKGYDPNLMMFQHISLIAEK
                     SNLQIMDFQPADRDNEKYYTWSVSCKGTFPGILNLVNGIENSSRFLKIESIEITSLED
                     GISVSLKVSGIRTLE"
     gene            complement(2141..>2480)
                     /locus_tag="PLW07_06480"
     CDS             complement(2141..>2480)
                     /locus_tag="PLW07_06480"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HOL50210.1"
                     /translation="KRLFLAQEECKKRIIWGRKFKIVASSLPEKMWLGKISLNQANKD
                     SSRVFVIEGYINSGISGTRKYLVDFMDNLKKNAGAEFSNISLAEIKNTGKSPGEQITT
                     FRIECGLKGN"
BASE COUNT          729 a          465 c          376 g          910 t
ORIGIN      
        1 acagttgtct ttactggcaa atttttgtgc tcaatatcaa gattttcaat aattgcctta
       61 acaatttcaa tattcgcggg agtatctttt ataacaagaa cgtttgttcg gttatcaaca
      121 gcaatgcttt tttcaaaacc aatgtttttt agttcagtag caatatcaga agcaaatgca
      181 tgtttcagtt gaaaacacgc acttacgggt ttttgtgaag aaatctttct ttgttctgaa
      241 tacacaatat aaatatcaga atcaggcaat ttttcgcgat ataatccatt tgcggcgagt
      301 atgctttcta ttgcttgctc tggagagata ttttcaagat atgctgtaat agacttatcc
      361 gaaatatcag tatccgaaat aagtttcatt cctgttttct tgctcaaaat ctcaagaaca
      421 agtgatagct ttgtctggtt aaaggttaat gattctattt tttgtgaagc aatacaatta
      481 agagaaagta taaaaatata taaaacaata attttcaaaa acaatgtttt catgtttacc
      541 tcacctgtta aatttttgta tcattctctg gtggaaaaat tattctttct tgaatataaa
      601 atgctgttct tacaaatgaa gtttctgaat actttctcaa accgtcacaa agtggaacga
      661 ggatatctgc atcagacgcc accccaatac gaactgtata catattcttt attttttcaa
      721 ccctcacatt ttcataacca cctgaaagaa gcatttttgc gaactcaatc gcctgcgtct
      781 tcttgttccc tgcaaatgaa gcaacctgaa tggtaagaat tttttgcgca ttttcattaa
      841 catcaggcaa cacaatcgac tgactcctgg taatctcctt ctgccttaca accggttgtg
      901 aagaaaccgt atcagttttt tcaacagttg caaacttagg tgaattgtct ttcaaaatgg
      961 agcgatatgt atttgctgtt gaggtagcaa tatctgtatc tcctgtttca attgcatcag
     1021 atagtttaat ttcactaatt ccagtacttt caatagaatc ggatttgaca taaagaataa
     1081 agtccttgtc cttatattta agaattactc tgtctggaag aatctttttg acaagatacc
     1141 cggattttgt ttcattttcg gatttgattt catcatcaag aatcaccatc ggtttttctt
     1201 tatcatacac tatcccggaa attttaaaat ccggtgcagg aatttcctga acaagaatac
     1261 tggtgtatgg cgattgtcct gttccaggag ataattgttt tgttcctggc ttcgtaaatg
     1321 gatctttaga tacacttaca gatgcaacct gttgttcctc ctcttcaatg gatttcatca
     1381 aagattgcaa cttgttgact gactggtcta tttcctgaaa actgaatact ttatcttcca
     1441 tagaggactt ctcattttcc tgtatgttta ttttttcaac tttgccgtca ggagatgaca
     1501 ttactattgt ttttggcttc actggcttca atgcgcgagt cagggtaaaa ataaaaatca
     1561 cgagtaacat caaaagtaga attaaacttt tcttattttt tttgtccatg tttcactcca
     1621 gtgttctgat acctgaaact tttaaactga ccgatattcc atcttccaaa cttgtaattt
     1681 caatggattc tatcttaaga aatctcgaag aattttctat accgttcacc agattgagaa
     1741 taccgggaaa agtaccttta caggaaacac tccacgtata atatttttca ttgtctctgt
     1801 cagccggttg aaaatccatg atttgtaagt tcgatttttc tgcaattaaa gatatgtgtt
     1861 gaaacatcat taaatttgga tcatatcctt tgttaatcca aaggaatcgg tcaagtttat
     1921 ttcccgcatt atcaatggtt tttcttatcg tattaatatg gtcaggatag tttttcaatt
     1981 tcatgtaatc gtattcaaga gattctttct catctctgta ttgttgtaaa attctgtgct
     2041 gctgctttat cacaacatta ttaataatca accacgtgcc cccataaagg atcagcatga
     2101 taacaagatg cgtttttatc gacgaagcat ggtttttcat tcaatttccc tttaatccac
     2161 attcaatcct aaatgttgta atttgttcac ctggcgattt acctgtattt ttgatttctg
     2221 caagagaaat atttgaaaat tcagctcctg catttttctt tagattatcc ataaaatcta
     2281 cgagatactt ccttgtacct gaaattccag aattaatata tccttcaatc acaaaaaccc
     2341 ttgaagaatc tttgttcgcc tgattgagtg aaatttttcc cagccacatt ttttctggta
     2401 gcgaactcgc tactatttta aattttcttc cccagattat tcttttttta cactcttcct
     2461 gagcaagaaa caatcttttt
//
LOCUS       DAORYU010000220         2468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_28516_length_2468_cov_5.086200, whole genome shotgun sequence.
ACCESSION   DAORYU010000220 DAORYU010000000
VERSION     DAORYU010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28516_length_2468_cov_5.086200"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PLW07_06485"
     CDS             complement(<1..222)
                     /locus_tag="PLW07_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50211.1"
                     /translation="MKKNQKLFLFLLCIFFGYSMCHASDIYNCYKTRERIVIDGKLQE
                     TSWKNARELIFKDMVNGSPASLKTTAKMMW"
     gene            complement(240..1223)
                     /locus_tag="PLW07_06490"
     CDS             complement(240..1223)
                     /locus_tag="PLW07_06490"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOL50212.1"
                     /translation="MKTKIAVVGLGIGQAHLQYYSQMPDVEIVGAMDVNSDVLKQVSE
                     KFGVKTFSSVGELLDATAPDGVSICTPPATHLKLTQIFAKKGIHILCEKPMAPRVSDC
                     EKMIDVCERNNVFLMLGFKKRFSPTYRFLKENFENDFGQPKWVLARFALGRVEKQWFW
                     DEKDGGGPIIENTVHMIDLLHFLCGRVKTVYAEGGNLFMPAFAPQIDTAIFTLRFEND
                     AIAGIGAGYASEWGFAKEQITFATDRVVCEVSGGFDRPRNIQYIYRNNPSVVQEKYFE
                     QPDGFPEEIRHFVDCIRNNTRPAVGGEEGLYALKVCRAVKKSIIKRKPVSI"
     gene            complement(1220..2035)
                     /locus_tag="PLW07_06495"
     CDS             complement(1220..2035)
                     /locus_tag="PLW07_06495"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOL50213.1"
                     /translation="MKFAINQATTMKTDFATDVKAYARAGFDSMEIWLPKLKEYLETT
                     TLADVKKLLKEEKIKPAGACYQNNLMFAQGELKQQAIKEFTEKLEICNVLEIPVLIVP
                     GDNPPSPVVESHYDEAAENLGKVADDARRYGVKLALEFIGTAKFLGCLSTAMKMVTSI
                     NHQNVGILFDIFHFFKGVSKTEDIDNVNGEVVFLVHLNDAMDKPREILTDRDRVLPGE
                     GVIPLETIIKKLQKINYHGYYSLELFNEQLWDQDPYSVAKKCFESLEKLGRKL"
     gene            complement(2032..>2468)
                     /locus_tag="PLW07_06500"
     CDS             complement(2032..>2468)
                     /locus_tag="PLW07_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50214.1"
                     /translation="TSNISPCCETDLPHQIPVNIGFRQSRRFDMLVGKESDHCRFLVR
                     VVLTGDISREQLFLRCNQFTAGSANQVKNVSVKELSNELTNSQKISGKVLKQVQAAEF
                     FVPARAIRNGRNVFVLRNDSDNEITIHGIETYVLPLNEGGKI"
BASE COUNT          703 a          567 c          415 g          783 t
ORIGIN      
        1 ccacatcatt tttgctgttg tttttaaaga agcaggagaa ccattcacca tgtccttgaa
       61 tataagttcc cgggcatttt tccacgaagt ttcctgtaat tttccatcaa taacaattct
      121 ttcccgggtt ttataacaat tatagatgtc tgaggcgtgg cacattgaat aaccaaaaaa
      181 aatacatagc agaaacagaa aaagtttctg gttttttttc atgatgtcct cttgaataat
      241 caaatggaaa ccggctttct tttaataatt gactttttta ccgccctgca caccttcaga
      301 gcataaagtc cttcttcccc accaacagca ggcctggtat tatttctgat acaatcaaca
      361 aaatgtctta tttcttcagg aaaaccgtcg ggttgttcaa agtatttttc ctgaaccaca
      421 gagggattgt tgcgatagat gtactgaata tttcttggtc tgtcaaaacc cccgctgacc
      481 tcacaaacaa ccctgtcagt ggcaaacgtt atctgttctt tcgcaaaacc ccattctgaa
      541 gcatagccag ccccaattcc agcaatggca tcgttttcaa acctgagtgt aaaaattgca
      601 gtatcaatct ggggcgcaaa tgcaggcatg aaaaggtttc caccttcagc atacactgtt
      661 tttaccctgc cacacaaaaa atgaagaagg tctatcatat gaaccgtatt ttcaataatg
      721 ggaccaccac cgtctttttc gtcccagaac cattgttttt ccactcttcc cagggcaaat
      781 ctcgcaagta cccacttcgg ctgaccaaag tcattttcaa aattttcttt cagaaacctg
      841 taagttggtg aaaatctttt cttaaaaccc agcatgagga aaacattgtt tctttcgcag
      901 acatcaatca ttttttcaca gtcagaaact ctgggtgcca ttggcttttc acacagaata
      961 tgaataccct tctttgcgaa gatttgcgtc agtttcagat gagtggcagg tggagtacag
     1021 atactgacac catcaggtgc tgtcgcgtcc agaagttcac caacagaaga aaaagttttt
     1081 acaccaaatt tttcagatac ctgtttcaaa acatcagaat tcacatccat ggctcctact
     1141 atttcaacgt caggcatctg tgaataatac tgaagatgtg cctgccctat gccaaggcca
     1201 acaactgcaa tttttgtttt cacagtttcc tcccgagttt ttcaagagat tcaaaacatt
     1261 ttttcgcaac agaatatggg tcctggtccc acaactgttc attaaaaagt tcaagagaat
     1321 aatatccgtg ataattgatt ttctgaagtt tctttatgat ggtctcaagc ggaatgacac
     1381 cttctcctgg aagcaccctg tccctgtcag tcagtatttc tctcggtttg tccattgcat
     1441 cattcaaatg cacaagaaaa actacctctc cattcacatt gtcaatgtct tcggttttac
     1501 tgacaccctt gaaaaagtga aagatatcaa acaatatccc tacattttga tgattaatac
     1561 ttgtaaccat cttcatcgct gtggaaagac atccaaggaa cttcgctgtg ccgataaatt
     1621 caagagcaag tttgaccccg tatcttctgg catcatcagc cactttgcca agattttctg
     1681 ctgcctcatc ataatgactt tcaacaaccg gtgatggagg attgtctcca ggaacaataa
     1741 gaaccggtat ttcaagaaca ttacagattt ccagtttttc tgtgaattcc tttattgcct
     1801 gctgtttcaa ttcaccctga gcaaacatca gattgttctg atagcaggcg cccgctggtt
     1861 taattttttc ttcttttaat agttttttta catctgcaag cgtggtcgtc tccaggtatt
     1921 cctttaactt tggtaaccat atctccatag aatcaaaacc agccctggca tacgccttca
     1981 catccgtcgc aaaatctgtt ttcattgtcg ttgcctgatt aattgcaaat ttcatatttt
     2041 tcctccttca ttaagtggta agacatacgt ttctattcca tgtattgtga tttcattgtc
     2101 agaatcattt cttaagacaa aaacatttct tccatttcgt attgctctgg ctggcacaaa
     2161 aaattcggct gcctgtacct gtttcagtac tttccctgat attttttgag aatttgtcag
     2221 ttcgttactt aattctttga ctgaaacatt cttcacctga tttgcagaac ctgccgtaaa
     2281 ctgattacat cgtaaaaata actgttcgcg agaaatatcc cctgtaagaa ccacacgcac
     2341 aagaaatctg caatgatcag attcttttcc aacaagcata tcaaaacgtc gtgattgacg
     2401 gaacccgata ttcactggta tctgatgggg caaatctgtt tcgcagcagg gactgatgtt
     2461 tgaagtaa
//
LOCUS       DAORYU010000221         2466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_28546_length_2466_cov_5.336375, whole genome shotgun sequence.
ACCESSION   DAORYU010000221 DAORYU010000000
VERSION     DAORYU010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28546_length_2466_cov_5.336375"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="PLW07_06505"
     CDS             <1..457
                     /locus_tag="PLW07_06505"
                     /inference="COORDINATES: protein motif:HMM:NF027666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4976 domain-containing protein"
                     /protein_id="HOL50215.1"
                     /translation="INSMLVSQYDLMPTILDYSGIKIPDDQYLCGKSFARALRGEKCG
                     DKEFVVVFDEYGPVRMIRTERWKYVKRYPYGPDELYDLKVDPSEEKNLINATGFEKIK
                     RQLCLQLREWFSKYTDSRVDGIYEPVTGKGQIGLCGVKSKGSISFNPID"
     gene            470..1933
                     /locus_tag="PLW07_06510"
     CDS             470..1933
                     /locus_tag="PLW07_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50216.1"
                     /translation="MATEKYFMDQPLQIGNTRQLLFDDFIIEDAWKVKRILHQPLKYF
                     KNPVIYKTEPWEAESVGHPTVFWDDDYGCYRMWYMSYSPKNYYTRGETGVIHCMCYAE
                     SKDGINWTKPVLDVCRFPGFEKTNVVYCGANGLTARGQVFKDETEKDPEKRYKMICLE
                     VFPLETGAEGKCSGLRMLYSPDGLRWTSEKAYLVLDYHSDTNNHIVYDRNNRRWLLYC
                     RPTIYSSGRSSNVRHHRRRVSVMVSEDLIHWSYPRTVLFPEEMDPPDYDHVYVVRYCN
                     YFIMFYAVMEGDTTGQKETKFAWSRDGFHWQRFYERRPFLPKGNEGSWDAGSVITYSM
                     VEQDDDLIFYYTGHHIGQHQYGISKNGIGIAFLRKDRFVEQRAEDETGYILTRELICQ
                     GKYLKLNTGFSSKPNRQQSIRVEISRRPEIGKHACFNYAFGGYGLEESMPISGDRMDA
                     VCRWKTKDNLSELIGKPVYVRFELVNMGLFSFHFSNE"
     gene            complement(1934..>2466)
                     /locus_tag="PLW07_06515"
     CDS             complement(1934..>2466)
                     /locus_tag="PLW07_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50217.1"
                     /translation="KITILNHQALEPVSFTGSLFRPDWKISTGKPVAVFENGSTAAVF
                     NQYGRGNAIIVGCSALTFSPYSNQSIDNPEFDKVRKLVTLAIEMAGVKKHCEVNIPGI
                     NCFVRDGENQTVLFLINSTGKKQSLRVTLDVPKKVVSAFDAKGEKLNCTQADGKLIFS
                     KTIEENDGDICVFRFQ"
BASE COUNT          779 a          418 c          558 g          711 t
ORIGIN      
        1 aattaacagt atgctggtat cccagtatga cttaatgcca acaatccttg actattctgg
       61 catcaaaatt cctgatgatc aatatctctg cgggaaaagt ttcgcaaggg ctttgagagg
      121 ggaaaaatgt ggagacaaag aatttgtcgt cgtgtttgat gaatacggtc ctgtaagaat
      181 gatcagaaca gaaaggtgga aatatgtaaa gagatacccg tatggacctg acgaacttta
      241 tgatttgaag gtagaccctt ctgaagaaaa aaatcttatc aatgcaacag gatttgaaaa
      301 aataaagaga caactctgcc ttcaacttcg tgagtggttt tcaaaatata ctgatagcag
      361 agtggatgga atatatgaac cagtgacagg gaaaggccag atcggacttt gtggggtgaa
      421 atctaaggga agtatttcat ttaatccaat tgattaacag gagcaattta tggcaactga
      481 aaaatatttt atggaccagc cgcttcaaat cggtaatacc agacagttac tctttgatga
      541 ttttattatt gaggatgcgt ggaaggtgaa aagaattctt catcagccgc tgaaatattt
      601 caaaaatcca gtgatttata aaactgaacc gtgggaagca gaatctgttg ggcatcccac
      661 agttttctgg gatgatgatt atggttgtta cagaatgtgg tatatgagtt attcacctaa
      721 gaattactat accagaggcg aaacaggtgt aatccattgt atgtgttacg ctgaaagcaa
      781 ggatggaatc aactggacaa aaccagttct tgatgtctgc aggttccctg gatttgaaaa
      841 aacaaatgtt gtttattgtg gggcaaatgg acttactgca aggggtcaag ttttcaaaga
      901 tgaaacagag aaagaccctg aaaaaagata caaaatgata tgtcttgagg tatttcctct
      961 ggaaactggc gcagaaggaa aatgcagtgg cttgagaatg ctgtattcac cggatggact
     1021 gagatggacc agtgaaaaag catatctcgt tttggattat catagcgaca ccaataacca
     1081 tattgtttat gatagaaaca accgcaggtg gcttctttat tgcagaccaa caatatacag
     1141 ttcaggcagg tccagtaatg tgaggcatca cagaagaagg gtgtcagtaa tggtgagtga
     1201 agatttgatt cactggtcgt atcccagaac agttttgttc ccggaggaaa tggacccacc
     1261 agattatgac catgtgtatg ttgttcgcta ctgtaattat ttcattatgt tttatgctgt
     1321 tatggaagga gatacgacag gccagaagga aacgaaattt gcatggagca gggatggttt
     1381 ccactggcaa cgattttatg aaagaaggcc ttttttacca aaaggcaatg aaggttcatg
     1441 ggacgctggt tcagtgatta cctactcaat ggttgagcag gatgatgacc tgatttttta
     1501 ctatacaggg catcacatcg gtcagcacca gtatggcatc agcaaaaatg gaattggtat
     1561 tgcttttctc agaaaggata gatttgttga acagcgcgca gaagatgaaa ctgggtatat
     1621 tctgacaaga gaattgatat gccaggggaa atatctgaaa cttaatacag gtttttcaag
     1681 caaaccaaac aggcagcaat ccatcagggt tgaaatttcc agaagacctg aaattggcaa
     1741 acacgcatgt tttaattatg cctttggtgg ttatggcctt gaagaatcca tgccaatttc
     1801 aggcgataga atggatgctg tgtgcagatg gaaaacaaaa gataatcttt cagaacttat
     1861 tggtaagcca gtatatgtaa ggtttgaact ggtaaacatg ggacttttca gttttcattt
     1921 ttcaaacgag tgattattga aatctgaaga cacagatatc tccgtcattt tcttcaatgg
     1981 tttttgaaaa tataagtttg ccatctgcct gggtacaatt cagtttttcg ccctttgcgt
     2041 caaatgctga gacaaccttt ttaggtacat caagggttac tcgaagggat tgttttttcc
     2101 ctgttgaatt gatcaaaaat aacactgttt gattttcgcc gtctcttaca aagcagttaa
     2161 taccagggat gtttacttca cagtgctttt ttactcctgc catttcaatt gcaagggtaa
     2221 caagttttct caccttatca aattcaggat tatcgatact ctggttgctg tacggactga
     2281 atgttaacgc agaacaaccc acaatgatcg cattgcccct gccatattga ttgaaaaccg
     2341 cagcagtcga accattttca aaaacagcaa ccggtttgcc tgtagatatc ttccagtcag
     2401 gtcgaaatag ggaaccagta aaagaaaccg gttcaagtgc ttgatgattg agaattgtga
     2461 tttttt
//
LOCUS       DAORYU010000222         2452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_28770_length_2452_cov_4.557781, whole genome shotgun sequence.
ACCESSION   DAORYU010000222 DAORYU010000000
VERSION     DAORYU010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28770_length_2452_cov_4.557781"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2017)
                     /locus_tag="PLW07_06520"
     CDS             complement(<1..2017)
                     /locus_tag="PLW07_06520"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOL50218.1"
                     /translation="MRRISAIVAVVLLSFCFSASGFTVVKDGKPMATIVVSKAVFDEA
                     TGQLSRYAKLKPEQKVKMAALDLQKYIEKMSGAKIDMVSEESQISGNMICVGKSKFTK
                     NVKNLKIPLGLTAERNEEGYTIFCSGNRLVLAGNDEGPYFGTYYAVAEFLRKLGIKWI
                     LPGDFGEVVPGKKDIEIAEMNLVDRPAFRVRSWWCNEPADMAAIHALWKIRNKMTIYD
                     RDIICIPGDSWLRKYLPDPKLKDTKPELFGRNFDGTVNVYMANLTNPETIKIVADKVV
                     QEFKKSEEKGEKINSIGFAPDDGLPMDHTPETMKLNQGFTDVFGREGVVTELSISEEW
                     FNFVNKVTEEVVKVYPDAIITTNGYANRSLPPEGIELHPNLGVMTAFIWADTLKPVTS
                     PKSWQGQVMGAQLKRWCELCKRVFIYEYNLTMLVSALTPVPQVKKMAINYSLFKKWGL
                     IGFINETKLPFFEEGIVTRYVRAQLMWNPDVDINALLDDFYSSWYGPASKPARNFWES
                     IEECLLETPLLGHEDRILPFVYTKELIEKLEKYVSEAERVANTDVIKTHVLVDRLTLE
                     HLKAYMAMKDAEFNAKWSDAVKYADKMIECRLALNKISPFLAMPPSLVARERYYSGDS
                     YFGILKRKALYQKLDGMTNGETGILVALAPKNVKFAIDEYGFGKHFRW"
     gene            complement(2080..>2452)
                     /locus_tag="PLW07_06525"
     CDS             complement(2080..>2452)
                     /locus_tag="PLW07_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="P-type; involved in magnesium transport into the
                     cytoplasm; Derived by automated computational analysis
                     using gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HOL50219.1"
                     /translation="MGPVSSLYDFITFGVLWFIFHASEQLFHTGWFLESLLTQTLVIH
                     VIRTGKKPFIESKPSPFLIFTSIYIVTVGLFIPFTPLGSRLGFVIPPFLYFVALFIIL
                     FSYLFTVQLVKQWFINRYGYE"
BASE COUNT          741 a          548 c          402 g          761 t
ORIGIN      
        1 accacctgaa atgcttacca aaaccgtact cgtcaattgc aaatttaaca tttttgggtg
       61 caagagcaac aagtataccg gtttctccat tcgtcatacc atcaagtttc tgataaagtg
      121 ccttccgttt caaaatccca aaatacgaat cccctgaata atatctttct ctggcgacaa
      181 gagatggggg catggcaagg aatggactga ttttgttcag agcaagcctg cattctatca
      241 ttttatcggc atacttgacc gcgtctgacc attttgcgtt gaattcagca tctttcatcg
      301 ccatatacgc tttcaaatgt tcaagcgtta atctgtcaac taatacatgg gttttaataa
      361 catctgtgtt tgcaactcgt tctgcttctg aaacatattt ttcaagtttt tctatcagtt
      421 cttttgtata aacaaatgga agaattctgt cttcgtgtcc aagaagtggt gtctccaaaa
      481 gacattcttc aatagattcc cagaaatttc ttgctggttt tgacgcaggc ccgtaccagc
      541 tgctataaaa atcgtcgaga agcgcattaa tgtcaacatc tggattccac atcaattggg
      601 ctctaacata tcttgtaaca attccttctt caaaaaatgg taatttggtc tcattgataa
      661 atccaataag tccccatttt ttgaacagcg aataattgat agccattttt ttaacctgag
      721 gaaccggcgt cagagcagaa acaagcatcg ttaagttgta ttcataaata aacacccgct
      781 tgcataactc acaccacctc ttcagttgcg ctcccataac ctggccctgc catgattttg
      841 gacttgtaac tggcttcaat gtatctgccc agataaaagc agtcatcacc cctaaattgg
      901 gatgaagttc aattccctca ggcggtagag aacgattggc ataaccattt gtggtaatga
      961 tagcgtcagg atataccttt accacttcct ctgtcacctt atttacaaaa ttaaaccatt
     1021 cttcgctgat tgaaagttca gtcacaacac cttccctgcc aaaaacatca gtaaaacctt
     1081 gattaagttt cattgtctca ggcgtgtggt ccataggtag accatcatca ggtgcaaaac
     1141 caattgaatt gattttctcc cctttttctt ctgacttctt gaattcctga acgactttgt
     1201 ctgcaacgat ttttattgtt tcagggtttg taagatttgc catgtacacg ttcactgttc
     1261 catcaaaatt tctgccaaac aattctggtt tcgtatcttt aagcttaggg tcaggaagat
     1321 attttctgag ccagctatca cccgggatgc aaataatatc cctatcatag attgtcattt
     1381 tattgcgtat cttccacaga gcatgaatgg cagccatgtc cgctggttca ttgcaccacc
     1441 acgaccgtac cctgaatgca ggcctatcaa caagattcat ctcagcaatc tcaatatctt
     1501 ttttaccggg aaccacctct ccaaaatctc ctggcaaaat ccattttatc ccgagttttc
     1561 ttaaaaactc agcgactgcg taatatgtac caaaatacgg tccctcatca ttccccgcaa
     1621 ggacaagccg atttccactg cagaaaattg tataaccttc ttcatttcgt tctgctgtaa
     1681 gccctaaagg aattttcaaa tttttgacat ttttcgtaaa cttactctta ccaacacaaa
     1741 tcatgtttcc tgaaatctgt gattcttcag aaaccatgtc aatttttgct cctgacattt
     1801 tttcaatata tttttgaagg tccaatgcgg ccattttaac tttctgttct ggcttcaatt
     1861 ttgcatatct ggacaattgt cctgtcgctt catcgaatac tgccttactc actacaattg
     1921 ttgccattgg ttttccatct ttgactactg taaaaccact tgcagaaaaa caaaatgata
     1981 ataaaacaac tgccacgatt gctgagattc gtctcatact cctcctttgg tttttgaatt
     2041 gtattttaca tcactttatc aatttgccaa ataataaaac tattcgtatc cgtatctgtt
     2101 gataaaccac tgctttacca gctgcacagt aaacaaatag ctgaataaaa taataaacag
     2161 agcgacaaaa tacaaaaagg gaggaattac aaaaccaaga cgcgagccca gtggtgtaaa
     2221 tggaataaac aatccaacag taacaatata aattgaagtg aatatcagaa aaggactggg
     2281 tttactttca ataaatggtt tcttacctgt acgaattaca tgaatgacaa gtgtttgagt
     2341 taaaagggat tcaagaaacc aaccagtatg aaaaagctgt tctgatgcat ggaatataaa
     2401 ccacaagaca ccgaaagtga taaagtcata aagggaactg acaggtccca tc
//
LOCUS       DAORYU010000223         2451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_28785_length_2451_cov_6.368114, whole genome shotgun sequence.
ACCESSION   DAORYU010000223 DAORYU010000000
VERSION     DAORYU010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28785_length_2451_cov_6.368114"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1936)
                     /locus_tag="PLW07_06530"
     CDS             complement(<1..1936)
                     /locus_tag="PLW07_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50220.1"
                     /translation="MKKFFAKICIIAILGCGGFSIAQTSSPYIGYVYPAGGQQGTVFQ
                     VVLGGQNLRSTNSVYFSDKGINATIVDYQGPSGPLNFLQTEELRRRIQEIRNKRAGKK
                     VEDTRSETEKAIKLPDIPELRDLENKTPGELQRIYEKYLNRENKPKPPVAEEVTIQVT
                     IAPDTQPGDYEVRLKTPSGLTNPLVFQVSEYNEFCCSYNQHRYQKDKVDRDTTILQAP
                     VVLNGRILPGKVNRFTLKLKKGQNIKITGYARKLIPYIADAVPGWFQAVLTLYDAKKN
                     EVAFSDDTAFDPDPIIKFQVPEDGNYILEIRDSIYRGREDFVYRIYVMDEPETGVAVE
                     KSDFDEKLSMLPDSIRNMPKYREIERKLRSAQYVSLPVLICGCINYPGDVDRYSFYGR
                     AGETVVIEVSGRRLGWPVDSLVKLKLMDKVIAWNDDIEKDFEHGILTHHADSYLMTKL
                     PTSGHYTVEIVDSQNRGGDHYKYFLRLSSPIHDFHLIVCPSQINISENGTSLITIYAI
                     KKDGWDGDIQLSLKNLPETFALDGATIPEGRNIIRLTLRTSSKFTNEILFPVLEGKAI
                     VDGKEVTRIATAAEEKMQAFAYIHPVPSETLLLAFSRSRLRQLKLDASNKEILKIHAG
                     GTATIACTAESWFRPTSSMSL"
     gene            complement(1942..>2451)
                     /locus_tag="PLW07_06535"
     CDS             complement(1942..>2451)
                     /locus_tag="PLW07_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013042686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HOL50221.1"
                     /translation="LVARRLVEIGVPYITINYPGWDTHKQHFQAMQRKLAEFDQGLSA
                     LIEDLSNRGLLDTTIVWCCGEFGRTPKIDWAPPWNGGRGHWGYCFSAVVAGGGFKGGV
                     VVGKSDSKGEYVAERPVYPEDLLRSILRLLGIDPDGQMPNDKGLDIKVMPYPQDYSGC
                     GELKEIMKN"
BASE COUNT          647 a          546 c          459 g          799 t
ORIGIN      
        1 ttaatgacat ggaagaagtg ggtctgaacc aggattctgc tgtacaggca atcgtagcag
       61 tgccacctgc atggattttt aaaatttcct tatttgatgc atcaagtttt aactgtctca
      121 atcttgaccg ggaaaaggct aacagcaatg tttctgatgg tacagggtga atatatgcaa
      181 atgcttgcat tttttcttca gcagctgtgg cgatacgggt aacttcctta ccatcaacaa
      241 ttgctttgcc ttccagtacc ggaaagagaa tttcgtttgt aaattttgat gatgtcctta
      301 acgttaacct gattatattt cttccctcag ggattgttgc cccatcaaga gcaaacgttt
      361 ctggaaggtt tttcaaactt aattgaatat ctccatccca gccatccttt tttattgcat
      421 agatggtgat taacgacgta ccattttcag agatattaat ctgagaagga caaacaatga
      481 gatgaaaatc atgtattggg ctactcaacc tgagaaaata tttgtaatga tctccgccgc
      541 gattctggct atcaacaatt tccacagtgt aatgtcctga tgtgggaagt tttgtcatta
      601 aatatgaatc agcgtgatgt gtgagaatgc catgttcaaa atccttttca atgtcatcat
      661 tccatgcaat aaccttatcc attagtttca atttcaccag tgaatcaact ggccagccca
      721 atcgtcttcc tgaaacttct atgacaacag tttctcctgc cctaccatag aaactatatc
      781 ggtcaacatc tccaggatag ttaatacaac cgcatatcaa tacaggcaat gaaacatact
      841 gagcagatcg cagtttgcgc tctatttctc tatattttgg catatttctg atactgtctg
      901 gcagcattga gagtttttca tcaaaatctg atttttccac tgcgactccc gtttcaggtt
      961 catccatcac ataaattcgg tagacgaaat cctctctacc acgataaatg gaatccctga
     1021 tttcaagaat gtaatttcca tcttctggca cctgaaattt aatgataggg tcagggtcaa
     1081 atgcagtgtc gtcactgaag gcaacttcat ttttttttgc atcgtaaagg gtcagtactg
     1141 cctgaaacca tccaggcaca gcatcagcaa tgtatggaat caattttctg gcatatccgg
     1201 ttattttgat attctgtccc tttttgagtt tgagtgtaaa cctgttgacc ttgcctggaa
     1261 gaattcttcc atttagtaca acaggcgcct gtaatatggt ggtatccctg tcgactttgt
     1321 ccttttgata tctgtgctgg ttataggaac aacagaattc gttgtattca ctgacctgaa
     1381 aaactaatgg attggtaagc cctgatggtg ttttgagtcg cacttcataa tcgcctggct
     1441 gtgtatcagg tgctattgta acctgtattg tcacctcttc tgccacaggt ggttttggtt
     1501 tattttccct gtttaagtat ttttcataga ttcgctgaag ttcgccaggg gtcttgttct
     1561 caaggtctcg caattcagga atatcaggaa gtttgatggc tttttctgtt tcacttcttg
     1621 tgtcttcaac ttttttcccc gcccgtttat tcctgatttc ctgtattctt cgtctcaatt
     1681 cttcggtctg cagaaaattc aatggtcctg aaggaccctg ataatcaacg atggtcgcgt
     1741 tgataccttt gtcagaaaaa taaacactgt ttgtgcttct taaattctgt ccaccaagta
     1801 ccacctggaa aacagttccc tgctgccctc ctgcaggata cacatatcca atgtatggac
     1861 tggatgtttg agcgatagaa aaaccaccac atccaagaat tgctattata catatttttg
     1921 caaaaaactt tttcattggc atcagttttt cataatttct ttcagttcac cgcatccaga
     1981 atagtcctgt gggtatggca taaccttaat atcaagccct ttgtcatttg gcatctgtcc
     2041 atcagggtca atcccgagta atcttaaaat gctccttaac aggtcttcag gatagacggg
     2101 tctttctgct acatattctc ctttgctatc tgacttacca acaaccactc cacctttgaa
     2161 tccaccacct gcgacaacgg ctgaaaaaca atatccccag tgtccacggc caccattcca
     2221 tggtggagcc cagtcaattt ttggggttct gccgaattcg ccgcagcacc atacaattgt
     2281 tgtgtcaagc aaaccccgat ttgacaaatc ttcaataagt gcagacagtc cctggtcaaa
     2341 ttcagccagt tttctttgca ttgcctggaa atgttgttta tgggtatccc atccagggta
     2401 attgattgtg atatatggca caccaatttc aacaagtcgt cttgcaacaa g
//
LOCUS       DAORYU010000224         2438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_28987_length_2438_cov_4.261016, whole genome shotgun sequence.
ACCESSION   DAORYU010000224 DAORYU010000000
VERSION     DAORYU010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28987_length_2438_cov_4.261016"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="PLW07_06540"
     CDS             complement(<1..361)
                     /locus_tag="PLW07_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOL50222.1"
                     /translation="MEQINLNTNAKNTWCIGCGNFAILNAIRIVLKELIAEGTPAENF
                     VLVSGIGCHAKIVDYININSFYSLHGRVVPVATAIKVANPSLNVICFSGDGDSYGEGL
                     EHLLFAAKRNVDITMIIH"
     gene            complement(364..2028)
                     /locus_tag="PLW07_06545"
     CDS             complement(364..2028)
                     /locus_tag="PLW07_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOL50223.1"
                     /translation="MVKEISILIGGKAGDGIDKASIVIAQMLSSIGYRIFVHRDYPSL
                     IRGGHTFSIIRASDIKIYAHKEEIDILIALNAETLDKHRKTLKNKCFYIYDSEQIKMH
                     ADACGTGIPINSILKEENAPELMKNSCLLGAFGKATGISWDIIEKTLKITIPKEIDLN
                     LKVAKKGYEYAKELISIEKLQQNSLPVLTGNEALGLGFVAGGLDAYVGYPMTPSSGLL
                     HFLAGLAKQFGLQVIHAENEISVIMMALGFAYTGKKSAVGTSGGGFCLMNEGLSLSGM
                     AEIPVVIVLGQRTGPSTGLPTYTGQTELFYAIYSGHGEFSRMVVAPGNCEQAYYWAAT
                     SLGLAWKYQMPSILLSDKTLAESMYSFDLNEIEKIDSNDFLIWDKQLPYKRYLNTDDG
                     ISPLCFVPEKNAVIKVNSYEHDEYGITTEDPVITKQMQEKRLRKLEFLKKDLQENYQC
                     VKVYGNLDSQNAILCWGSNTPVCVEVSEKLGFKVIQPVVIHPFPEEQFKKAMIGVRNL
                     ISVECNATGQLTDLIRQFGYSVNNKILKYDGRPFSVNELENNLKAL"
     gene            complement(2022..2396)
                     /locus_tag="PLW07_06550"
     CDS             complement(2022..2396)
                     /locus_tag="PLW07_06550"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOL50224.1"
                     /translation="MQGYKDKKIALVGVSSDQSKIGYKIFEDLLANNFNVVGVNPKNG
                     LILQQHIYTSLKEINPLPDIVVTVVKPEVTVEIIEQCHALGIKNVWMQPGSESNQAIE
                     KARIYGIEVKYGKCFMSENGIW"
BASE COUNT          736 a          505 c          365 g          832 t
ORIGIN      
        1 tatgaataat catcgttatg tcaacattcc gtttggcagc aaacaataaa tgttccaacc
       61 cttcaccata agaatctcca tctccagaaa aacagatcac attaagagat ggatttgcaa
      121 ccttaattgc tgttgcaaca ggaacaaccc ttccgtggag tgaataaaaa ctattaatgt
      181 tgatataatc aacaattttt gcatgacaac caattcctga tacaagtaca aaattttctg
      241 ctggcgtacc ttctgcaata agttctttca gtacaatcct tattgcattt aaaatagcaa
      301 aattaccaca gccaatacac caagtatttt ttgcatttgt gttaagatta atttgttcca
      361 ttgttacagt gcctttaaat tgttttcaag ttcattcaca gaaaaaggtc taccatcgta
      421 ttttagaatt ttattgttaa cagaatagcc aaactgacga attaaatctg ttaattgtcc
      481 tgttgcattg cattccacag aaatcaaatt tctcactcca atcatggctt ttttaaattg
      541 ttcttcagga aatggatgta ttacaactgg ttgaataact ttaaaaccga gtttttcact
      601 cacttctacg cacaccgggg tattagaacc ccagcacagt attgcatttt gagaatccag
      661 atttccatat acctttacac attgataatt ttcctgaagg tcttttttta aaaactctaa
      721 ttttctcaac cttttttctt gcatttgctt tgtaatcaca ggatcttctg tggtaatacc
      781 atattcatca tgttcataac tattcacttt tatcactgca tttttctcag gaacaaagca
      841 caacggtgag atgccatcat ctgtattgag atatcgttta tagggtaatt gtttatccca
      901 tattaaaaaa tcattactat caattttttc aatttcattt aaatcaaaac tatacatgct
      961 ttcagcaaga gttttatctg acaacaaaat agaaggcatt tgatatttcc atgccagacc
     1021 aagagaagtg gcagcccagt agtatgcctg ttcacaatta cctggtgcga caaccattcg
     1081 actgaattca ccatggccgc tgtatattgc ataaaataat tctgtttgtc cagtataagt
     1141 gggtagccct gtgcttggac ctgtcctctg tccaagaaca attacaacag gaatttctgc
     1201 catacctgat aaactcaacc cctcattcat taaacaaaac ccaccgccag atgttccaac
     1261 agcagatttc tttcccgtgt aagcaaatcc aagagccatc atgatcaccg atatttcatt
     1321 ttcagcgtgt atcacctgaa gaccaaactg tttagcaagt cctgcaagaa aatgcaaaag
     1381 cccggacgac ggagtcatcg ggtaaccaac gtaggcatca agtcctccag caacaaaacc
     1441 aagacccagg gcttcatttc ctgttaacac aggcagagaa ttttgttgaa gtttttcaat
     1501 actaatcagt tcttttgcat actcatagcc cttctttgct acctttaggt ttagatcaat
     1561 ttcttttggt attgttattt tcaaggtttt ttcaataata tcccaactta ttcctgttgc
     1621 ttttccaaaa gcgccaagaa gacaggaatt tttcatcaat tcaggggcat tttcttcttt
     1681 taaaattgag ttaattggaa tgcctgttcc acaggcatct gcatgcattt taatttgctc
     1741 tgaatcataa atataaaaac atttattttt gagagttttg cgatgtttat caagagtttc
     1801 agcattaagg gcaatcaata tatctatttc ttctttgtga gcataaattt ttatatctga
     1861 agccctgata attgaaaatg tatgtccacc tctaattaag gaaggatagt ccctgtggac
     1921 aaaaattcta tagccaatag aagacagcat ctgggcaatc acaatacttg ctttatctat
     1981 tccatcaccc gctttcccac cgatcaatat agaaatttct ttcaccatat tccgttctca
     2041 ctcataaaac attttccata ttttacttca attccataga ttctggcttt ttcaattgcc
     2101 tgatttgatt cagaacctgg ctgcatccac acatttttta tgccgagggc atggcattgt
     2161 tcgataattt caacagtaac ttctggtttt accacagtta ctacgatgtc cggcaatgga
     2221 ttaatttctt taagagatgt ataaatgtgt tgctgcaaaa ttaagccatt tttgggattt
     2281 actccaacaa cattgaaatt attggcaaga aggtcttcaa atattttata gccgatttta
     2341 ctttgatcgg acgatacacc gacaagcgca atttttttat ccttatagcc ctgcattatt
     2401 tcctcaggtt cgtttacctt ggttctgttt tattttat
//
LOCUS       DAORYU010000225         2413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_29383_length_2413_cov_2.997031, whole genome shotgun sequence.
ACCESSION   DAORYU010000225 DAORYU010000000
VERSION     DAORYU010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29383_length_2413_cov_2.997031"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1583
                     /locus_tag="PLW07_06555"
     CDS             <1..1583
                     /locus_tag="PLW07_06555"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOL50225.1"
                     /translation="FQLEPNVYVELTTALLLNTKYGFDELLKLWPKKVKYFGLYDYWA
                     VYDWIRDRLPSGRTGNTQYITEKLPYYIHHGICNLSAESGNSWGSQGLGYYLAARVLW
                     NSKTDVKALKQDFYDKAFGPAADIMKRYYERIDLASKPLVGPTFYRLCIDDLLEAETV
                     ANQYPEVLARIEQLKQYHVFLYLRRKTYDERDLTAEERKKWAFEMLKWNYRMRNTYMT
                     FWSFFAVQTTQQLSQEFNEPTWWWWKMWSEHRQDEIPYRIKDPITTEEINQWLEIAKN
                     DYGEGIVFEEIPFSKKLVAPLVEKLETKSAQSIFMSQGGFHIALASIKGEPLQLTIMH
                     GTIYKNFPDGKYTLYDSDGKIIEEGTIGYGEHNLLLKVPAPGVYSLKYDDFAAGCRLI
                     PSEQTRTAFILTKGEAFKVYNHNYLYFYVPKGTKEIRFYALRSWPVGICQPDGQWVGE
                     EKPIYHHPRSIKADGSYRKIVVPEGMDGKVWKCVDFLSGTFYFFNIPNLLFVRETDVI
                     VPEDVAKKDNLKWGLKNE"
     gene            1576..>2413
                     /locus_tag="PLW07_06560"
     CDS             1576..>2413
                     /locus_tag="PLW07_06560"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOL50226.1"
                     /translation="MSEKRPNVLWIMADQLRADCLGFMGNPVVQTPNLDMLASQSVVF
                     TNAFCQSPACTASRASLFTGRYPPTVRVRGMGILPPTETTITEVLRRHGYHTAASGKI
                     HLTPEQYTKYQLKSDVPIIDWKKFAADALIDNIPQDSVKENYGFEHYVGCEDILLGNF
                     YKWLDENYPELSGKKPVPLFEEGPRDLRVSPYPSEAHPTTFIANNAEAFIRLLKNKEP
                     WFVFCSFIAPHHPFQAPSDQIARYDEKDIPLPEEKGGVDASFIPEPAKSAIGEITRYS
                     KDI"
BASE COUNT          782 a          426 c          534 g          671 t
ORIGIN      
        1 attttcaact tgaacctaat gtttatgttg aactgacaac agcgctgcta ttgaatacaa
       61 aatacgggtt tgatgaactg ttaaaactgt ggcccaaaaa ggtcaaatat tttggtttat
      121 acgattactg ggctgtttat gactggatac gggacagact tccatcaggt cgtacgggta
      181 atactcaata tattactgaa aaactgccat attacattca tcatggcata tgcaatcttt
      241 ctgctgagag tgggaatagc tggggaagtc aggggctggg atattatctt gctgcaaggg
      301 tcttgtggaa ctcaaagaca gatgttaagg cattaaaaca ggatttttat gataaagcat
      361 tcggacctgc agcagatatt atgaaacgtt attatgaaag aatagatctt gccagtaaac
      421 ccttggtagg acccacattt tatcgtctgt gcattgatga ccttcttgaa gctgaaacgg
      481 tagcaaacca gtatcctgaa gttctggcaa gaattgagca attgaaacag taccatgttt
      541 ttctctacct tcgcagaaaa acgtatgatg aaagagattt gactgctgaa gaaagaaaaa
      601 aatgggcttt tgaaatgttg aaatggaatt acaggatgcg aaatacatac atgacattct
      661 ggagtttttt tgcagtccag acgacccaac aactttctca ggaatttaat gaaccgacgt
      721 ggtggtggtg gaagatgtgg agtgaacata gacaggacga aatcccatac agaataaaag
      781 acccgattac aactgaagaa ataaatcagt ggctggagat tgcaaaaaac gattatggag
      841 aaggtattgt ttttgaagaa ataccattca gtaagaaact tgttgcgcct ctggtggaaa
      901 aattagaaac aaaatcggct cagagtattt ttatgagtca gggagggttt catattgctc
      961 ttgccagtat taaaggagaa ccacttcaat taacaatcat gcatggcacc atttataaaa
     1021 attttcctga tggaaaatac accctgtatg actcagacgg gaaaattatt gaagagggaa
     1081 ctatagggta tggagagcac aatttattat tgaaagtacc agcaccagga gtttattcct
     1141 taaagtatga tgattttgct gctggttgca ggctaattcc ttcagagcaa acaagaaccg
     1201 catttattct tacgaaaggc gaggctttca aggtatataa ccacaattat ctgtattttt
     1261 atgttccaaa ggggacaaag gaaatcagat tttatgcttt aaggtcatgg cctgttggca
     1321 tatgtcagcc tgatgggcaa tgggtcggtg aggaaaaacc aatctatcat catccacgtt
     1381 ctatcaaggc agatggctca tacaggaaaa ttgttgtacc tgaaggaatg gatggaaagg
     1441 tatggaagtg tgttgatttc ctttcaggaa cattttattt ctttaacatc ccaaatcttt
     1501 tgtttgtgag agaaacagat gtcattgtgc cagaagatgt ggcgaaaaag gataacttga
     1561 aatggggctt gaaaaatgag tgaaaaaaga cctaatgtat tgtggattat ggcagaccag
     1621 ttgagggcag attgtctggg atttatggga aatcctgtag ttcagacccc aaatcttgac
     1681 atgcttgcca gccagagtgt tgtattcacg aatgcttttt gccagagtcc tgcctgtacg
     1741 gcatcaaggg caagtttgtt taccggaaga taccccccta ccgtaagagt acgagggatg
     1801 ggaattctac caccaactga aacaacaatc actgaagttt taagaaggca tggctaccat
     1861 actgctgcat caggaaaaat tcatcttacc cctgagcagt acacaaaata tcaattaaaa
     1921 tctgacgttc ctattattga ctggaaaaag tttgcagcgg atgcactgat agacaatatt
     1981 ccgcaggata gtgtgaaaga aaattatgga tttgagcatt atgttggttg cgaggatatt
     2041 ttacttggga atttttacaa atggcttgat gaaaattatc ctgaactttc tggtaaaaag
     2101 ccagtaccat tgtttgaaga aggaccacga gaccttcggg tttcacccta tccttctgag
     2161 gcgcatccta caacatttat cgcaaacaat gcagaagcat tcatacgatt actgaaaaac
     2221 aaagagccat ggtttgtttt ttgttccttt atcgccccac accatccatt ccaagctcct
     2281 tctgaccaga ttgccaggta cgatgaaaaa gacataccct tgccagaaga aaaaggagga
     2341 gttgatgcct cgttcatacc agaacctgca aaaagtgcaa tcggtgaaat tacaagatac
     2401 agcaaagata ttc
//
LOCUS       DAORYU010000226         2400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_29599_length_2400_cov_5.080597, whole genome shotgun sequence.
ACCESSION   DAORYU010000226 DAORYU010000000
VERSION     DAORYU010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29599_length_2400_cov_5.080597"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="PLW07_06565"
     CDS             <1..482
                     /locus_tag="PLW07_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50227.1"
                     /translation="AKCCANYSLKIWTNNIGLINTLLTKYERFFTFNFVGIVSGEFSS
                     KNMSIINLTLPVSEIPKIIIGSPAISSKGMSADNISTQHQVDSLIKRTNEIIVVADGS
                     KIGKTETYLTRSSRMIRIDIKKGRRYLLITTKPQETQELFEKEISKLQNLGINVKIL"
     gene            492..665
                     /locus_tag="PLW07_06570"
     CDS             492..665
                     /locus_tag="PLW07_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50228.1"
                     /translation="MKRNLKCSMCGKAVCLDEVFAFYPEDDSLICKNCFGASTISPNK
                     KPVLQKRVCKYVD"
     gene            677..>2400
                     /gene="glmS"
                     /locus_tag="PLW07_06575"
     CDS             677..>2400
                     /gene="glmS"
                     /locus_tag="PLW07_06575"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOL50229.1"
                     /translation="MCGIAGYTGKQEAISKVIRSLKRLEHRGYDSCGIAFTNEKVLCV
                     VKTVGGINKLCKELTIDKDKTCPVICIGHTRWATHGKVSVENAHPHVDCVNRIAVVHN
                     GIIENYQELKKSLLLNKHNFISATDTEVIPHLIEHFLMEGKTDEQAFFDTIKKIRGSF
                     AVVTIFTHDNRIFFTQKNSSLLVYKHNEGTCLISDPVGLPEEATSVFAVNDGEWGVVS
                     PDHILLYDNKGNERSLKNVSYLKNSNNETQHFFIKEILEQSSVVVSSLRSVISFFERT
                     GNNFFEGINKILITGCGSSYHAALIGRYYFETIALIDTNVELASELSSCRMYNKKNTL
                     VIALSQSGETRDTVSAVKKFDTAETLAITNVENSLLTRLTSHHILMLAGPEISVAATK
                     SFTAQVVCLYVISLIAAFSRRAISAEKKNEMIVDVLKLSGVSEKIFSLASQLKNISKF
                     LKDSHTIFILGRGINYPVALEAALKLKEVSGVHAEGLSSAEMKHGPIALISETTPVFF
                     IAPMDETYEKTLNNIEEIKARGGKTFVITTENNQDIKQICDNVICIPDSKKLSPICSV
                     IPFQLLSY"
BASE COUNT          818 a          445 c          448 g          689 t
ORIGIN      
        1 ttgcgaaatg ctgtgcaaat tattcgttaa aaatctggac aaacaacatt ggtctcatca
       61 ataccctgct gacaaaatat gaaagatttt tcacgtttaa tttcgtagga attgtctctg
      121 gagagttctc aagcaaaaat atgtctatca tcaacctgac actacctgtg tcagaaatac
      181 caaaaatcat tattggctct cctgcgatca gcagtaaagg aatgagcgct gataatatct
      241 caacacaaca ccaggttgat agtttaatca aaaggaccaa cgaaattatt gttgtcgccg
      301 acggttcaaa gatagggaaa acagaaacat acctgacgag atcctcaagg atgatccgga
      361 ttgatatcaa aaaaggaaga aggtatcttc tgattaccac aaaaccccag gaaacgcagg
      421 aattatttga aaaagaaatt tcaaaattac aaaaccttgg aatcaacgta aaaattctgt
      481 aaggaggtag catgaaaaga aatctgaagt gttcaatgtg tgggaaggcg gtctgtcttg
      541 atgaagtttt tgctttttac cctgaagacg attctctcat ttgcaagaat tgtttcggcg
      601 cttcgaccat aagtccgaat aaaaaacccg ttctccaaaa aagagtttgc aagtatgtcg
      661 attaacgatt aatactatgt gcggaatcgc tggttacaca ggaaaacagg aagcaatatc
      721 aaaggttatc agatcactta aacgacttga acacagggga tacgattcct gtggtattgc
      781 gtttacaaat gaaaaggtac tctgtgtggt aaaaactgtc ggaggaatca ataaactttg
      841 caaagagtta acaatagaca aggacaaaac ctgtcctgtt atctgtatag gtcataccag
      901 gtgggcaaca cacggaaagg taagcgtgga aaatgctcat ccacatgttg attgtgtaaa
      961 ccgtattgca gtcgtccaca acggaattat tgaaaattac caggaattaa agaaatccct
     1021 tctcctcaat aagcacaatt ttataagtgc cactgataca gaagtcatcc cgcatttgat
     1081 cgagcatttt ctcatggaag gaaaaacaga tgaacaggca ttttttgaca cgattaagaa
     1141 aataagaggg agttttgcag ttgtgactat tttcacccat gacaatcgta ttttctttac
     1201 tcaaaaaaat tcaagtttac ttgtttataa acataacgaa ggaacatgtc tcatttctga
     1261 ccctgttgga cttcctgaag aagcaacctc tgtctttgct gtcaatgacg gcgaatgggg
     1321 agtagtttct ccggaccaca ttctgctgta cgataacaaa ggaaatgaaa ggtcattgaa
     1381 aaatgtttct tacttaaaaa atagcaataa tgaaacccag cactttttca taaaagaaat
     1441 actggaacaa tcaagtgtag ttgtttcttc gcttcggagt gttatttctt tttttgagcg
     1501 aacaggtaac aatttttttg aagggataaa caaaattctg attactggat gtggtagctc
     1561 ctatcatgcc gcacttatcg gcaggtatta ttttgaaaca atcgcattga ttgacacaaa
     1621 tgttgaactt gcctcagaat tatcttcctg cagaatgtac aataagaaaa atacacttgt
     1681 tattgcgctg tctcaatcag gtgaaaccag agatacagta tctgccgtta aaaaatttga
     1741 cactgctgaa accctggcaa taaccaacgt agaaaattct cttctcacaa gactcacctc
     1801 tcatcacatt ttgatgctgg caggtccaga aataagtgtg gctgccacca agtcattcac
     1861 tgcacaggtc gtttgtctgt atgttatcag tttgatcgcg gcattttcaa gacgtgccat
     1921 ttcagccgaa aaaaagaatg aaatgattgt ggacgttttg aaattatccg gtgtttcaga
     1981 aaaaattttt tctcttgctt ctcaactgaa aaatatcagc aaattcctca aagattctca
     2041 taccatcttt atacttggaa gaggaataaa ttatccagtt gctcttgaag cagccctaaa
     2101 acttaaagaa gtatcaggtg ttcatgcaga aggattatca tctgcagaaa tgaagcatgg
     2161 tcctattgca ttaatttctg aaacaacacc tgtatttttt attgctccca tggatgaaac
     2221 ttatgaaaaa acattgaaca atattgaaga aattaaagcc cggggaggaa aaacatttgt
     2281 gatcacaaca gaaaataatc aggacatcaa acaaatctgc gataacgtaa tatgtatacc
     2341 tgactcaaaa aaactttctc ctatttgttc agttattcca tttcaacttc tttcgtattt
//
LOCUS       DAORYU010000227         2387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_29814_length_2387_cov_3.328902, whole genome shotgun sequence.
ACCESSION   DAORYU010000227 DAORYU010000000
VERSION     DAORYU010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29814_length_2387_cov_3.328902"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="PLW07_06580"
     CDS             <1..551
                     /locus_tag="PLW07_06580"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HOL50230.1"
                     /translation="GKLTQKAWESDVQVIIEGPGHIPLHQVIPNVHLAKTICHGAPLY
                     LLGPLVTDVAAGYDHITSAIGGALAAWAGADFLCYVTPSEHLALPTIDDVEDGVVASK
                     IAAHAADIAKGHPGARAWDDELSIARYKRDWKRQEELVINPERFKKIRQSQKIYSEDV
                     CNMCGKYCAMKIMDEIIKKSGL"
     gene            523..1062
                     /gene="def"
                     /locus_tag="PLW07_06585"
     CDS             523..1062
                     /gene="def"
                     /locus_tag="PLW07_06585"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOL50231.1"
                     /translation="MKSLKNLDCEMTTRRIRKYPAEVLRKKAEEIVDFGKKTQDLFRD
                     LEETLEKSSGLGLAAPQIGVSSRAFIAVDRDKNKIYRIVNPEIVDMDSEQEIDMEGCL
                     SFPEIFFAISRPRKILVSGYTNTGRKIKIQAEGILARCFLHEMEHLDGVLIIDHASKQ
                     EKEFWKETLSRLTGTPANK"
     gene            1059..>2387
                     /gene="nadB"
                     /locus_tag="PLW07_06590"
     CDS             1059..>2387
                     /gene="nadB"
                     /locus_tag="PLW07_06590"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOL50232.1"
                     /translation="MIPDYLISINPVAVSIEDVDCLIIGSGIAGLRAAIELAGMRTLV
                     ACKNDPYDGSTYNAQGGIAVALSENDSPESHLHDTVAAGCFVNDISAVTVLVNEGIER
                     VRELIDWGCKFDKRGKDFDFTREAAHSFPRILHAMGDATGKEIVHTLLSKIGSIPEIV
                     LQGKLFLIDLVSDGEKITGGLFHDNTTHNLVFVRAKKTIAASGGFGQLFQETTNPSAI
                     TGDLQAAAFRKGALLGDLEFYQFHPTALYMAGVPRFLISESVRGEGAVLVNAGGERFM
                     KHYHTNMELAPRDVVSRAIVAEMRRTNTNCVYLDLRKMKPQFVIKRFPGIYRFCLEYG
                     LDITSNLIPVRPCAHYAMGGIVTDIDGKTNLENFFACGEVACTGVHGANRLASNSLLE
                     GLVFGARAGRKIREELGSFHHIKTAALPEYKIRKQNIPFIDIYDLQRSIKS"
BASE COUNT          721 a          419 c          586 g          661 t
ORIGIN      
        1 tgggaaaact tactcaaaag gcatgggaat cagatgttca ggtgattata gaagggccag
       61 gtcatattcc gcttcatcag gtgattccaa atgtgcatct tgcaaaaacc atctgtcatg
      121 gtgcgccact gtatcttctt gggccacttg ttactgatgt tgcagctggc tatgaccaca
      181 ttacatctgc aataggaggt gctcttgcgg cgtgggcagg cgctgatttt ctatgctatg
      241 tcactccgag cgaacatctt gcgcttccca caattgatga tgttgaagat ggggtcgttg
      301 cttcaaaaat tgcagcgcat gctgcagaca ttgcaaaggg tcatccagga gcaagagcat
      361 gggatgatga actttcaatt gctcgttata aaagggactg gaaacgccag gaagaacttg
      421 tgattaatcc tgaaagattc aaaaaaataa gacagtcgca aaagatttat tctgaagatg
      481 tttgcaatat gtgtggaaag tactgcgcga tgaagattat ggatgaaatc attaaaaaat
      541 ctggattgtg aaatgaccac aagaagaatt agaaaatatc cggcagaggt gctgagaaag
      601 aaggcagaag aaattgtgga ttttggaaag aagacccagg atttattcag ggatcttgaa
      661 gaaacgcttg aaaaatcatc cggtctgggt cttgctgctc cgcagatagg tgtcagcagt
      721 cgggcattta ttgctgttga cagagacaaa aataagattt acagaatagt gaatccggaa
      781 attgtagaca tggattcaga acaagaaatt gatatggaag gttgtttgag ttttcctgaa
      841 atttttttcg cgatatcaag acccagaaaa attctggtga gtggttacac aaataccgga
      901 aggaaaatca aaattcaagc agaaggaatt ctggcaagat gttttctgca tgagatggaa
      961 catcttgacg gcgttctgat tattgatcac gcgagtaaac aggaaaaaga attctggaag
     1021 gaaacgctca gtcgtctcac cggaacacct gctaacaaat gattcctgat tatctgatat
     1081 caatcaatcc tgtggcagta tcaatagaag atgttgattg tcttattatt ggtagcggta
     1141 ttgcaggcct gcgggcagcc atagaacttg caggcatgcg cacacttgtt gcctgcaaaa
     1201 acgatcctta tgatggcagt acatacaatg cgcagggcgg aattgcggtt gctctatcag
     1261 aaaacgatag tcctgaatct catctgcatg atactgttgc tgccgggtgt ttcgtaaatg
     1321 atatatccgc tgtgactgtt cttgttaatg aaggtattga aagggtcagg gaactgattg
     1381 attggggatg taagtttgac aaaagaggca aagattttga ttttaccagg gaagccgcgc
     1441 attcttttcc gaggatatta catgcaatgg gtgacgcaac gggaaaagag attgtgcata
     1501 cgcttctttc aaaaattgga agtattcctg aaattgttct tcagggaaaa ctttttctta
     1561 ttgacctggt ttctgacggt gaaaaaatta cgggtggcct gtttcatgat aacacgacgc
     1621 acaatctggt tttcgtgcgt gcaaaaaaga caattgctgc ttcaggaggt tttggtcagc
     1681 tttttcagga aaccactaat ccttcagcaa ttactggtga ccttcaggcg gcagcatttc
     1741 gtaaaggggc actcttgggt gatttagaat tttatcaatt tcatccgact gccctgtaca
     1801 tggcgggagt cccaaggttt ttaatctctg aatcagtcag gggcgagggt gcagtccttg
     1861 tgaatgcagg tggtgaaagg tttatgaaac attatcatac gaatatggaa cttgccccaa
     1921 gagatgttgt ttcaagggca attgtggcag aaatgagaag aacaaacaca aattgtgtat
     1981 atcttgacct gagaaaaatg aagccacagt ttgtgatcaa acggtttcct ggaatatata
     2041 gattctgtct tgaatatgga cttgatataa caagcaatct tatacctgtc agaccatgcg
     2101 cgcactatgc catgggaggc attgtgacag acattgatgg taaaacaaat cttgaaaact
     2161 tttttgcctg cggcgaagtt gcatgtacag gtgttcatgg ggcgaacagg ctggcttcca
     2221 attctctgct tgaaggattg gtttttggag caagagcagg aagaaaaatt agagaagaat
     2281 taggttcatt tcatcatatc aaaaccgctg ccttgccgga gtacaaaatc agaaagcaaa
     2341 atattccatt tattgacatt tatgacttac agaggtccat taagagt
//
LOCUS       DAORYU010000228         2328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_29927_length_2381_cov_4.694755, whole genome shotgun sequence.
ACCESSION   DAORYU010000228 DAORYU010000000
VERSION     DAORYU010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29927_length_2381_cov_4.694755"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="PLW07_06595"
     CDS             <1..487
                     /locus_tag="PLW07_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOL50233.1"
                     /translation="GSNSIGLFHPFYKDKSVKFIGVEAAGKGLDTDYHSATLCKGNPG
                     ILHGSFSYVLQDKYGQIHPTHSVSAGLDYPGTGPEHSFYKDAGRAEYYGVTDQQVLNA
                     FHVLSEKEGIIPALESAHALAYLLENRSRFNPDDIVIVCLSGRGDKDVNEVARIEGVQ
                     L"
     gene            484..1278
                     /gene="trpA"
                     /locus_tag="PLW07_06600"
     CDS             484..1278
                     /gene="trpA"
                     /locus_tag="PLW07_06600"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOL50234.1"
                     /translation="MNIKHKFKLLAQEKRAGLIIYITAGCPDINFTEEFVQKLQDIGV
                     DLIEIGVPFSDPVADGPIIQASSQKALENRINLDSIFGLCDRLKSRLSIPYLLMTYYN
                     PVYQYGLENFARDCAETGVAGVIVPDLPFEETAQLKTELKKFTIDQISFLSSTTSPER
                     RNKILKNASGFIYYIAVHGVTGTRDFLPEETYQDVARLREQAPLPVAVGFGISNKQQI
                     LRLKNCADGIIIGSFVMKEIMAGNHVNLLNTLKEFRRILNNPDENF"
     gene            1302..2081
                     /locus_tag="PLW07_06605"
     CDS             1302..2081
                     /locus_tag="PLW07_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50235.1"
                     /translation="MELCLYNSPMGKQDKKPGKKSSGKLIGLIVIILLAGGAYFGYQK
                     YQAYQKQLAEQKAKEEELKKQQLAEQQKRKELEQAKQKFEQLLSQMREALKMKNYNLV
                     RELAEQARKLALVYNFSTDEIDKILREMNLAIAMAKLSQLEKIKDPYAHLYVRNELKK
                     ITRYPEIAKRWDRLWKKSFQDEYTVLLDLAETTSGKVKSGDNPEINYSLSKTYLKKAK
                     AIVAAGRAIPDKERENALLQKQSDGYLSSIGRSFQPSGLYR"
     gene            2084..>2328
                     /locus_tag="PLW07_06610"
     CDS             2084..>2328
                     /locus_tag="PLW07_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOL50236.1"
                     /translation="MNKKINIGIIGYGTVGSNTALILRDKKVLFNRRTGIEFQVKKIC
                     DVDWKTKRGWMPPVTMRTSDYVDIIEDPDIDVVVSVF"
BASE COUNT          771 a          370 c          529 g          658 t
ORIGIN      
        1 aggtagcaac agtatagggc tttttcatcc attttataaa gacaaaagtg taaaatttat
       61 tggagttgaa gctgctggaa aaggacttga tacagattat cattctgcaa cactgtgcaa
      121 aggaaaccct ggaatattac atggcagttt cagttatgtg cttcaggata aatatgggca
      181 gattcatcca acccattctg tttctgcagg acttgattat ccgggaacag gacctgaaca
      241 cagtttttac aaggatgcgg gaagggcaga atattatggt gtgacagacc agcaggttct
      301 taacgctttt cacgttctga gtgaaaaaga gggtatcata ccagcccttg aatcagccca
      361 tgccctggcg tatttattag aaaatagaag cagatttaat ccagatgata ttgtaattgt
      421 atgcctgtct ggaaggggag acaaggatgt gaacgaagtt gccagaattg aaggggttca
      481 actgtgaaca tcaaacataa gtttaaatta cttgcacaag agaaaagagc cggtttaatt
      541 atttatatca cagccggttg tccggatatc aattttacag aagaatttgt ccagaaatta
      601 caggacattg gagttgattt gatagaaatt ggagtgccgt tttctgatcc tgttgcggat
      661 ggtccgatta ttcaggcgtc ttcccagaag gcactcgaaa acaggattaa tttagattca
      721 atatttggtt tgtgtgacag gttgaaatca agattatcaa ttccatatct cttgatgacc
      781 tattacaacc ctgtgtatca atatggactt gaaaactttg cgcgcgattg cgcggaaaca
      841 ggggttgcag gtgttattgt tcctgacctt ccgtttgaag aaactgcgca gttaaagaca
      901 gaattaaaaa aatttactat cgaccagatt tcatttctca gttcaacgac atctccagaa
      961 agaagaaata aaatcctgaa aaatgcttca ggatttatct attacattgc agtgcatggt
     1021 gtaaccggaa caagggattt tttgcctgaa gaaacttacc aggatgtagc gcgattgcga
     1081 gaacaggcac ccttgcctgt ggctgtcggg tttggaattt cgaataaaca gcagattttg
     1141 cgtttgaaga attgcgctga tggtattatt ataggtagtt ttgttatgaa ggagataatg
     1201 gcgggtaatc atgtaaattt gctgaataca ttaaaagaat ttcgacgcat cctgaacaat
     1261 cctgacgaga acttttgagg gtgcggtgtt atttgacctc agtggaactt tgtctgtata
     1321 atagtcctat gggtaaacag gataaaaaac cagggaaaaa atcttcaggg aagttgattg
     1381 ggctaattgt aataatcctt cttgcgggtg gtgcgtattt tggttatcaa aaatatcagg
     1441 cataccagaa gcaacttgct gagcagaagg caaaagagga ggagttgaaa aaacagcaac
     1501 ttgcagaaca acagaaacgt aaagaactgg aacaggcgaa acagaaattt gaacaacttc
     1561 tatcacaaat gagagaagca ttgaaaatga aaaattacaa tcttgtcaga gaacttgctg
     1621 aacaggcaag aaaacttgcc cttgtttata atttttctac tgatgaaata gataaaattc
     1681 tgagggaaat gaatcttgcc atagcaatgg caaaactttc gcaactcgaa aaaataaaag
     1741 atccctatgc gcatctttat gtcagaaatg agttaaagaa aattacgaga tatcctgaaa
     1801 ttgcaaaaag atgggatagg ttgtggaaaa agagttttca ggatgaatat actgtactgc
     1861 tggatcttgc tgaaactact tccggtaaag tgaagagtgg tgataatcct gaaattaatt
     1921 actcattgtc caaaacatat ctgaaaaaag caaaggcaat tgttgcggca ggcagggcaa
     1981 tcccggataa ggaacgtgaa aatgctttgc tgcaaaagca aagtgatggg tatctttctt
     2041 ccatcggaag aagtttccag ccttctggtc tttatcgctg attatgaaca aaaaaatcaa
     2101 tattggtatc ataggatatg gaactgttgg cagtaacacg gcattgattt tacgtgataa
     2161 gaaggtgctt tttaacaggc gcacaggaat agagtttcaa gtaaaaaaaa tctgtgatgt
     2221 cgactggaag acaaaaagag gatggatgcc gccagtaaca atgagaacat ctgattatgt
     2281 tgatattatt gaagatcctg atattgatgt tgttgtttca gttttttt
//
LOCUS       DAORYU010000229         2372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_30070_length_2372_cov_5.526543, whole genome shotgun sequence.
ACCESSION   DAORYU010000229 DAORYU010000000
VERSION     DAORYU010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30070_length_2372_cov_5.526543"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2266)
                     /locus_tag="PLW07_06615"
     CDS             complement(<1..2266)
                     /locus_tag="PLW07_06615"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOL50237.1"
                     /translation="MRTVFLIDANNIIYRSFFAIRGFTTSTGQPTNAIFGFVSTILKI
                     LKDYKPEYLAVAFDTPAPTFRHRLLEQYKITRKPMPEELSAQMPVMKEILKAFGITMV
                     EKESFEADDLLACLALKFSGAGHRVIIVSGDKDILQLLSEKINVLNPASWELMDVEHF
                     MEKYSFHPRNIVDYLALCGDASDCVPGVPGIGEKTASDLIKEFGTIENLYENVESVQG
                     KRKQLLIDGKENVFLSKRLVQLSCDTGLEMNIEDIKRKSVDAEKIRQIFQSLEFRKFE
                     PQLEEIFGLNVNSSIPQDAFAIGDRIFNFNEILANPFQFAAELTDEHLVKLGTNIKEK
                     IKLLMSKNFHLSPPYFDFHIASFILGKSLSGTDIFQIYNAYLELFKQHQMDFLIHSIE
                     MPLIEVLAFMEMNGVLVDTEYLDVLKKEYESEMAALQEKIYHIAGEVFNINSPSQVAT
                     ILFDKLGLPPKRKTKTGYSTDTTVLEQLRSRHKIVELILQYRELSKLCSTYIDGFIPF
                     INSADSRIHPEYHQTVASSGRLVCHNPNLQSIPVKTEKGGKIRKIFIAPENATLYSFD
                     YSQIELRILAHFSKDKNLIEAFQNNRDIHIETAKQLFPLQQDCLFGTDEIEKYRRIAK
                     TINFGIIYGMNEYGLAQRLACPVEEARVFIDCYFARFPLVKAYIQKAVEDAEKKGYAS
                     TLSGRRRYLPEIHSQNRNQKEFAKRVAVNMPIQGTAAELIKLAMIKIHDLIKKEKMKS
                     KMILQVHDELVFEIS"
BASE COUNT          700 a          444 c          427 g          801 t
ORIGIN      
        1 ttgatatttc aaaaacaagt tcatcgtgaa cctgaagtat cattttactt ttcatttttt
       61 ccttctttat taaatcatga atttttatca ttgcaagttt tatcaattct gctgctgtac
      121 cctgtatcgg catattcact gcaacccttt ttgcaaactc tttctgattt ctgttttgag
      181 aatgaatttc tggtagatac cttcttctgc cagaaagcgt gcttgcataa cccttttttt
      241 ctgcatcttc aactgctttc tggatgtatg cttttacaag cggaaatctg gcaaaataac
      301 aatctatgaa tactcttgcc tcttcaacag gacaggcaag tcgttgagca agaccatatt
      361 cgttcattcc gtaaataatg ccgaaattga ttgtctttgc aatgcgacgg tatttttcta
      421 tctcatcagt accaaaaagg cagtcctgct gcaagggaaa tagttgtttt gctgtttcaa
      481 tgtgaatatc tcgattgttc tggaatgctt caatgagatt tttgtccttt gaaaaatggg
      541 caagtattct taattcaatc tggctgtagt caaaagaata aagggttgcg ttttctggag
      601 caataaaaat ttttcttatt tttccaccct tttcagtttt tacaggaatt gattgaagat
      661 ttggattgtg acagacaagt cgaccgctgg aagcaactgt ctgatgatac tctggatgaa
      721 ttctgctgtc agcagaattg ataaaaggaa taaatccatc aatatatgta ctgcagagtt
      781 ttgatagttc tctgtattga agaatgagtt ccacaatttt atgccgactg cgaagttgtt
      841 ccagcacagt tgtatctgtg gaataacctg tctttgtttt tctttttggt ggaagaccga
      901 gtttatcaaa caaaattgtt gcaacctgag acggagaatt gatattgaac acttcgcctg
      961 caatgtgata aattttttcc tgtaatgcag ccatctcact ttcatattct tttttcaaaa
     1021 catcaagata ttctgtatca acaagcactc cattcatttc cataaaggca aggacttcaa
     1081 tcaggggcat ctcgatggaa tggataagaa aatccatctg gtgttgtttg aaaagttcaa
     1141 gatacgcgtt gtaaatttga aaaatatctg ttccagaaag tgatttgcca agaataaagg
     1201 atgcaatgtg aaaatcaaaa tatggaggcg aaagatgaaa gttttttgac ataagaagtt
     1261 tgattttttc cttaatgttt gtgccaagtt ttacaaggtg ttcgtctgtt agttcagcag
     1321 caaattgaaa agggttggca agtatctcat tgaaattaaa aattctgtct cctatggcaa
     1381 atgcatcctg cgggatactg gaattaacat tgagcccaaa aatttcttct aactgaggtt
     1441 caaattttct aaactcaagt gattgaaaaa tttgcctgat tttttcagca tcaacacttt
     1501 tcctttttat gtcttcaata ttcatttcaa gaccagtgtc acaggaaagt tgaacaagtc
     1561 gcttacttaa aaaaacgttt tcctttccat caatgagcaa ctgttttctt ttcccctgaa
     1621 cactttcaac attttcataa agattttcta ttgttccaaa ctctttaatt aaatcagatg
     1681 cagttttttc acctattccc ggcacaccag gaacacaatc agaagcatca ccgcaaagcg
     1741 ccagataatc cacgatattt ctgggatgga aggaatattt ttccatgaaa tgttctacat
     1801 ccatcaattc ccagctggct ggatttagaa cattgatttt ttctgataac aattgaagga
     1861 tatctttatc tcctgaaacg ataatcacac gatgccctgc gccactgaat tttaatgcaa
     1921 ggcaggcaag aaggtcatct gcttcaaagg actctttttc aaccattgtt attccgaatg
     1981 ccttcagaat ctctttcatt acaggcatct gtgcggaaag ttcctctggc atgggttttc
     2041 tcgtaatctt gtattgctca agcaaacgat gcctgaatgt gggagcaggt gtatcaaacg
     2101 caaccgcaag atattctggc ttataatctt ttaaaatttt taatatcgtt gagacaaatc
     2161 cgaaaattgc gtttgttggt tggccggttg aagttgtaaa acccctgata gcgaaaaaag
     2221 acctgtagat aatgttgttt gcgtctatca ggaaaacagt cctcattgag cacgtgcctt
     2281 ataggaaacc agttccattt caactttaac aaaaaacggc ttttgagcat tgcgagaaat
     2341 ttgaagataa ccgtatctca agaactctct tt
//
LOCUS       DAORYU010000230         2356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_30360_length_2356_cov_3.796610, whole genome shotgun sequence.
ACCESSION   DAORYU010000230 DAORYU010000000
VERSION     DAORYU010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30360_length_2356_cov_3.796610"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="PLW07_06620"
     CDS             complement(<1..890)
                     /locus_tag="PLW07_06620"
                     /inference="COORDINATES: protein motif:HMM:NF002326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HOL50238.1"
                     /translation="MRTRKDWEEIEEKTLAPYAVKSNQHLGRKYPERQHPLRTSFQRD
                     RDRIIHSTAFRRLEYKTQVFIYHEGDYYRNRLTHTIEVQQIARSIARNLGLNEDLTEA
                     ISLAHDIGHTPFGHKGEQALNEIMQKFGKSFEHNLHGLRVVDILEIRYPQFEGLNLTY
                     EVREGIIKHKTSYDSPAISEFTEHVSPSLEAQVVNLADEVAYTSHDLDDGLKSGILEE
                     KELAPLGIWQNVIKRINRNVDEQILRTSIVRTLINLLVEDLIETSEQLIKQNSIRSVE
                     DARKLPQLVGFSPTVYTQYD"
     gene            complement(887..1678)
                     /locus_tag="PLW07_06625"
     CDS             complement(887..1678)
                     /locus_tag="PLW07_06625"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOL50239.1"
                     /translation="MERKTGYCLKNMGQISIIEAFIFLFLISGCTLSVATKYPEKQST
                     KKNTTNKQVLASKQQEQGVWHIVKKGENLYRISLYYEVSQQDIKKTNNMGSDSLFVGQ
                     KLFIPGTKKKAPLFALTPDLTQNQPETTVIEQPISETKILKEPEYAWPVEGKIICTYG
                     ELGNKGIDILTSPGSSVKAAKDGKVVFVGTTTKYGETIIIEHENGMFSVYGHDMIVRV
                     KEGQSVKKGAIIGEMKNSSQARRYLHFEIRYKNNPVNPLNLLEKK"
     gene            complement(1623..2021)
                     /locus_tag="PLW07_06630"
     CDS             complement(1623..2021)
                     /locus_tag="PLW07_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013682908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HOL50240.1"
                     /translation="MPQRKIFVVHEHFAKKHHFDLRLEINGVLKSWAIPKEIPSKKGE
                     KRLAVEVDDHPLEYASFEGEIPEGTYGAGRVSVWDSGHFLLLKKDKKAIEVDLRGEKL
                     RGIYVLLLFREENGKKNWLLFKKYGADKHH"
     gene            complement(2023..>2356)
                     /locus_tag="PLW07_06635"
     CDS             complement(2023..>2356)
                     /locus_tag="PLW07_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOL50241.1"
                     /translation="GGKVPDTMEDLIKLPGVARKTANVVLNQAFGKNQGIVVDTHVRR
                     VSQRLGLTKNTDPVKIEQDLMKIIPQKHWGSFSFRLVQHGRKICAAKKPLCNICAFVN
                     MCPSAQVK"
BASE COUNT          584 a          476 c          441 g          855 t
ORIGIN      
        1 tgatcgtatt gagtgtaaac tgtgggactg aatccgacca gttgcggtaa ttttcttgca
       61 tcttccactg atctgatgct gttttgtttg atgagttgtt cgcttgtttc aatgaggtct
      121 tcaaccagta aattgataag tgttctgact atagatgttc ttaaaatctg ttcgtcaaca
      181 tttctgttga ttctttttat tacattttgc catattccaa gaggggcaag ctccttttct
      241 tcaaggatac cacttttaag tccatcatca agatcatggc tggtataagc aacctcatca
      301 gcaaggttaa caacctgtgc ttctaaactg ggagatacat gttcagtgaa ttctgatatg
      361 gcaggactat cataacttgt cttatgcttg ataatgcctt ctcttacttc ataggtaaga
      421 ttaagtcctt caaattgtgg atacctgatt tcaaggatgt cgacaaccct gagtccatgc
      481 agattgtgtt caaaactttt tccgaacttt tgcatgattt cattaagtgc ctgttcaccc
      541 ttgtgtccaa acggtgtatg accaatatca tgggcgagag agatggcttc tgtaaggtct
      601 tcgttcagtc ctaaatttct tgctatggaa cgggcaattt gttgaacttc tatggtatga
      661 gtaagacggt ttctataata atcgccttca tgatagatga aaacctgggt cttatattcg
      721 agtcgcctga acgctgtgga atgaataatt ctgtctctat cacgttgaaa tgatgttctt
      781 aatggatgct gtctttctgg atattttctg ccgaggtgtt gattgctttt tacagcataa
      841 ggtgcaaggg ttttttcttc tatttcttcc cagtcctttc tggttctcat tttttctcca
      901 gcaaattcaa tggattcaca gggttgtttt tgtatcttat ttcgaaatgc aggtatcttc
      961 tcgcctggct cgaatttttc atctccccaa taatagcacc tttttttacg gattggcctt
     1021 cttttactct gacgatcatg tcatgtccat aaacagaaaa cattccgttt tcatgttcaa
     1081 tgataattgt ttccccatac tttgtcgttg taccgacaaa gacgacctta ccatctttag
     1141 ctgcttttac agaactaccg ggggaagtga gaatatcaat tcctttgttc cccagttctc
     1201 cataggtaca gatgattttt ccttcaacag gccaggcata ctcaggttcc tttagaattt
     1261 ttgtttctga tataggttgt tctattactg tcgtttctgg ctggttctgg gtaagatcag
     1321 gagtaagagc aaacagaggt gccttctttt ttgtccctgg aataaaaagt ttttgaccga
     1381 cgaataagct gtctgagccc atattattgg tttttttgat atcctgttgt gaaacctcat
     1441 aatacaggga aattctgtaa agattttcac cctttttcac tatatgccag acaccctgtt
     1501 cttgttgttt tgatgccaga acctgtttat ttgttgtatt cttttttgtt gattgttttt
     1561 caggatattt tgtggcaaca cttagcgtgc aaccagaaat taaaaataaa aagataaatg
     1621 cttcaatgat gcttatctgc cccatatttt ttaaacagta accagttttt ctttccattt
     1681 tcttccctga aaagtaagag aacataaatg cccctgagtt tttcgccccg taaatctacc
     1741 tctatggctt ttttatcctt ttttaaaagt aaaaagtgtc ctgaatccca tactgatact
     1801 ctgcctgcac catatgttcc ttctggtatt tcaccttcaa atgatgcgta ttctaaggga
     1861 tgatcgtcaa cttctacagc aaggcgcttt tcaccttttt ttgaaggtat ttcctttgga
     1921 atagcccagg attttaaaac accattgatt tccaggcgca aatcaaaatg gtgttttttt
     1981 gcaaagtgct catgaacgac aaatattttt ctctgtggca tattatttta cctgtgcaga
     2041 aggacacatg ttgacaaaag cgcaaatatt gcacaacggt ttttttgcag cgcaaatttt
     2101 tcttccgtgt tgaacgagac gaaaagaaaa acttccccag tgtttctggg gtatgatttt
     2161 cattaagtcc tgttctattt ttaccgggtc tgtatttttc gtaagaccaa gccgctgact
     2221 cactcttctt acatgggtgt caacgactat tccctggttt tttccgaatg cctgattgag
     2281 cacaacattg gcggtttttc tcgcgactcc aggcagtttt ataaggtctt ccatcgtatc
     2341 aggaacctta ccgccg
//
LOCUS       DAORYU010000231         2346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_30528_length_2346_cov_9.653863, whole genome shotgun sequence.
ACCESSION   DAORYU010000231 DAORYU010000000
VERSION     DAORYU010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30528_length_2346_cov_9.653863"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="PLW07_06640"
     CDS             <1..595
                     /locus_tag="PLW07_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50242.1"
                     /translation="GYRRVNGVIKKFEDTGVTLVLFSRDGKSKTILPVSRPFIPQGIS
                     GHEAWLNNKPAFIFSTSPADLPYDDGNRCGNLLIYEIGDQKPHVAAHAPETYFGHVST
                     SACGGYWCCDAWHWKPGDENVCAGCSPDIAVGSIKTGKFAFVCKVGGFWTGYEIVHSH
                     PYLSADNKCVIFTSTRTGFPQVFAAMLPDGFLENLDS"
     gene            658..2292
                     /locus_tag="PLW07_06645"
     CDS             658..2292
                     /locus_tag="PLW07_06645"
                     /inference="COORDINATES: protein motif:HMM:NF014598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HOL50243.1"
                     /translation="MNALFLMVLVVVSFIVAFRFYGRYLEKLWDVSSEKKTPAHTKYD
                     GIDYIPAKHWIVLFGHHFSSIAGAGPILGPVIAGVVWGWGPALLWIVFGSIFLGTVHD
                     FSSLILSLRYDGQSVGQITKDILGERVKLVFSFFLWLSLVLVIAVFAAVTAKTFVEEP
                     RIVLPSFAFIFIAVVFGFLVYRKNANLIFTTLFSLVLMAFFLYAGKHIPIIFAVENPV
                     KAWIVILLIYSFIASVLPVNILLQPRDYLSSFILFFGLFFGFLGIIVSHPSTNAPFFV
                     SFNSSKGPLLPMMFVTIACGAISGFHGLISSGTTSKQLPDEKYSRHIAFGSMLTEGVL
                     AAIALLCVCAGLYWNSPFQELNYPFLMQKGDWIGTFAIGYGNIVSKIFTSDIGKLIAM
                     IMVNSFVLTTLDTATRITRYITSEIFGESFKIKIFNNRFFSTLLILVFASYLAFGAWE
                     KIWPVFGASNQLVAALILFVCSCFLMMKKKNSLSVLIPALIMLVITMMALIYQANTFY
                     KSHHLLLGNISVILVLLAAFIVVEGVKVIKKIRNST"
BASE COUNT          627 a          441 c          485 g          793 t
ORIGIN      
        1 gggttacagg cgagtcaatg gggtgataaa aaaatttgaa gacactggag taacacttgt
       61 tctcttttca agggatggta aaagtaaaac aattcttcct gtgtcccggc catttattcc
      121 gcagggaatt tcaggacacg aagcatggtt aaacaataag ccagcattta ttttttcaac
      181 atctccggct gatttaccct atgacgatgg aaaccgatgt ggaaatcttc ttatttatga
      241 aattggagac caaaaaccac atgttgctgc ccatgcgcct gaaacatact ttggacatgt
      301 ttcaacttct gcctgcggag ggtactggtg ttgtgatgca tggcactgga agccaggaga
      361 tgaaaatgtt tgcgcaggat gttcaccaga cattgccgtt ggctcaatca aaacaggaaa
      421 gtttgcattt gtatgcaaag tgggtggttt ctggactggc tatgagattg tacacagcca
      481 tccctattta agtgctgaca ataaatgcgt gatttttaca tccacccgta ctggctttcc
      541 ccaggtattt gctgcgatgc ttccagatgg gtttcttgaa aatcttgaca gttgaacata
      601 aattgttgta tattttttaa cccaaattta tacttgaatc aataacaagg agaaataatg
      661 aatgcacttt ttttaatggt gcttgttgtc gtcagtttca ttgttgcctt cagattttat
      721 ggaagatacc ttgaaaaact atgggatgtc agttctgaaa aaaagacccc tgcccacacc
      781 aaatacgatg gaattgatta tattcctgca aaacactgga ttgttctttt cggacatcat
      841 ttttcttcaa tcgcaggcgc aggaccaata cttggacctg tgattgcagg agttgtgtgg
      901 ggatggggac cagcgctact gtggattgtt tttggctcaa ttttccttgg tacagtacat
      961 gatttttcat cgcttatttt aagtttaaga tacgatggtc agagtgtggg ccagattaca
     1021 aaagacatac ttggggaaag agtaaaactg gtgttttctt ttttcctgtg gctctcactg
     1081 gttcttgtta ttgctgtgtt tgcagctgtg acagcaaaaa catttgttga agaaccaaga
     1141 atagttcttc cttcttttgc cttcattttt attgctgttg tttttggttt tctcgtttac
     1201 agaaaaaatg caaatctcat ttttaccaca ttgttttcac ttgtgctgat ggcttttttt
     1261 ctttatgcgg gaaaacacat accaatcata tttgcagtag aaaatccggt aaaagcatgg
     1321 attgtgatac tgcttattta tagttttatt gcaagtgtct tgcctgtcaa tattttactt
     1381 cagccaaggg actatttgag ttcttttatc ctgtttttcg ggttattttt tgggtttctt
     1441 ggaattattg tttcccatcc ttcaacaaac gcaccatttt ttgtctcttt caattcttca
     1501 aaagggccac tgttaccaat gatgtttgtt acaattgcct gcggcgcaat cagtggtttt
     1561 catggactta tttccagtgg aacaacatca aaacaactgc ctgatgaaaa atatagcagg
     1621 cacattgcat tcggaagcat gcttacagaa ggtgtgcttg cggctatcgc tctcttgtgt
     1681 gtctgtgcag gattatactg gaatagccca tttcaggaat taaactatcc cttcctgatg
     1741 caaaagggag actggatagg cacatttgcc attggctacg gaaacatcgt gtcaaaaatt
     1801 ttcacttctg acattggaaa actgattgca atgattatgg tcaatagttt tgtcctgacg
     1861 acccttgaca cagcgacaag aattaccagg tacatcacct ctgaaatttt tggcgagtcg
     1921 ttcaaaataa aaatctttaa caacagattt ttttcaacct tgttgattct ggtttttgcg
     1981 tcctatcttg cctttggagc atgggaaaaa atctggcctg tatttggcgc ttcaaatcag
     2041 cttgttgcag cacttattct ttttgtgtgc agttgtttcc tgatgatgaa aaagaaaaat
     2101 tcactttcag tgctgatacc agcattgatt atgcttgtca ttacaatgat ggcgcttatt
     2161 taccaggcaa acacttttta caaaagccat cacctgcttc ttggcaatat cagtgtgatt
     2221 ctggttttac tggcagcttt tattgttgtt gaaggtgtca aagtgataaa aaaaatcagg
     2281 aatagcacct gattattccc actctattgt ggctggtggc tttgaagtga tatcatacac
     2341 aaccct
//
LOCUS       DAORYU010000232         2336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_30709_length_2336_cov_5.469969, whole genome shotgun sequence.
ACCESSION   DAORYU010000232 DAORYU010000000
VERSION     DAORYU010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30709_length_2336_cov_5.469969"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1014
                     /locus_tag="PLW07_06650"
     CDS             <1..1014
                     /locus_tag="PLW07_06650"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOL50244.1"
                     /translation="RKFLPEKIIDVHTHLWRKRDYPVQKKHDPRFVTWPSRVAEENPI
                     EDLIETYKVLLPGKQVVPVMFPNLPSGNNLDAINSYVSDCSKKTGYPALIFSDPEWAS
                     EELEERIIQGNFIGAKSYMSMLPETIPPNNINIFDYFPKHQLEIHDKNGWIVMLHIPK
                     EGRLKDPENLKNLCEIDRIYENIQVIVAHVGRAYCNHDAGNAFEILKKTKRIMFDISA
                     NTNENIFSMLIECVGPDRILFGSDMPITRMRMRRIEKDGIYVNLVPKGLYGDVSRDKH
                     MAEIDGIEAENLTFFLYEEIDAFRKACERQKLKDDDIEKIFYKNALNMIEKAKKWRKY
                     VDH"
     gene            1001..2332
                     /locus_tag="PLW07_06655"
     CDS             1001..2332
                     /locus_tag="PLW07_06655"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HOL50245.1"
                     /translation="MLIINNPYEIKNGLWLKGNFHIHTTRSDGTLGPQDTINQYAELG
                     HDFLSFSDHDNIMTDNEYKLLDSRNMILISGIEISAGGPHLLYIDSEQNITCYPERQK
                     VLNEIEKIYRETGRGFAVVNHPDWEQQFNHCTIEQLMEWTGYLGMEIYNGVIGRLDGS
                     QYATNKWDILLSKGKKVWGFANDDAHRKGDHGLGWNVVFARKKTKDAIIEAIFKGNFY
                     CSTGVLIRHIECDGDTIYIETENAKKIAAIQNVGKRFKVVYGNCIKVEIPQDAKYVRF
                     ECWGQAEQMAWTQPFFVSLNEVAVELEYVKDWLISNLLDISGLDETNPDAALKFAKIK
                     TACEPAGTALSGFVDTRKQSNGQDGILYLVSEVQFENDTKALLSLGYDGPIRVWLNGE
                     EIFYGPGTNPAIRDQLKIYTRVKKGTNKLVIAFDTNGGKAWGIFCKIRPAC"
BASE COUNT          819 a          369 c          495 g          653 t
ORIGIN      
        1 agaaaatttc ttcctgaaaa aatcattgat gttcatactc acctctggag aaaaagggac
       61 tatcctgttc aaaaaaaaca tgatccgagg tttgtaacct ggccatcaag agttgcagaa
      121 gaaaatccca ttgaagacct tattgaaact tataaagttc tacttccagg caaacaggta
      181 gttcctgtca tgtttccaaa cttaccatca ggaaataacc tggacgctat caatagttat
      241 gtcagcgatt gttcaaaaaa aaccggatac cctgctttga ttttcagcga ccctgagtgg
      301 gcttctgaag aacttgaaga aagaattata caagggaatt ttattggggc taaaagttat
      361 atgagtatgc ttcctgaaac aatacctcct aataacatca atatttttga ttattttcca
      421 aaacatcaac ttgaaattca tgataaaaat gggtggattg tgatgctcca tattccaaaa
      481 gaagggagat tgaaggaccc ggaaaattta aagaatctct gtgaaattga cagaatttat
      541 gaaaatattc aggtcattgt tgcgcatgtt ggaagagctt actgtaatca tgatgcggga
      601 aatgcctttg aaatattgaa gaaaacaaaa agaattatgt ttgatatcag cgccaatacc
      661 aatgagaata tcttttccat gctgattgaa tgtgttggac ctgacagaat tctttttggt
      721 tcagacatgc ccattacaag aatgaggatg agaagaattg aaaaagatgg aatttatgta
      781 aatcttgtgc caaaaggact ttatggagac gtgtcccggg ataaacatat ggctgaaatt
      841 gatggaattg aagcagaaaa tcttacattt tttctgtatg aagaaatcga tgctttcaga
      901 aaagcatgcg aaaggcaaaa gctcaaagat gatgacattg aaaaaatttt ttataaaaat
      961 gctctgaaca tgattgaaaa agcaaaaaag tggaggaagt atgttgatca ttaataatcc
     1021 ctatgaaata aaaaatggtc tgtggctgaa aggtaatttc catattcata caacaagaag
     1081 tgatggcaca ttaggtcctc aggatacaat caaccaatat gctgaacttg gacatgattt
     1141 tctttcattt tcagaccatg acaatataat gacagacaat gagtataagt tgctcgacag
     1201 tagaaatatg attttgattt ctggtattga aatttcagcc ggtggcccac atttactcta
     1261 cattgactcc gaacagaaca ttacctgtta tcctgaaaga cagaaggtat tgaatgaaat
     1321 tgagaaaatc tacagggaaa ctggtcgtgg ttttgcggtt gttaaccatc ctgactggga
     1381 acaacaattt aaccactgta caattgagca acttatggaa tggacaggat atcttggcat
     1441 ggaaatttat aacggagtaa ttggacggct tgatggaagt caatatgcca ccaacaaatg
     1501 ggatattctt ctttctaaag gtaaaaaggt atggggcttt gccaatgacg atgcacatag
     1561 aaaaggcgac catggtcttg gatggaatgt tgtttttgca agaaaaaaaa caaaggatgc
     1621 tattattgaa gcaattttca aagggaattt ttactgttcg acaggcgttc tgataagaca
     1681 tattgaatgt gatggcgata caatttacat agaaacagaa aatgcaaaga aaattgctgc
     1741 aattcaaaat gttgggaaaa gatttaaggt tgtttacgga aattgtataa aggttgagat
     1801 acctcaggat gcaaaatatg tcaggtttga gtgctgggga caagcagaac aaatggcatg
     1861 gacacagccc tttttcgttt cactcaatga agttgctgtg gaacttgaat atgtaaagga
     1921 ctggttgatt tcaaatcttc ttgatatcag cggccttgat gaaacaaatc ctgatgcagc
     1981 attgaaattt gcaaagatta agacagcctg cgagccagca ggcaccgcac tttctggatt
     2041 tgttgacaca agaaaacaat caaatggtca agatggaatt ctttatcttg tttcagaagt
     2101 acaatttgaa aatgatacaa aggcactttt atctcttggg tatgatggcc cgataagagt
     2161 atggctaaac ggagaagaga tattttatgg gccaggcaca aatcccgcaa tcagggacca
     2221 gctaaaaatc tatacccggg taaaaaaggg aaccaataaa cttgtcatcg catttgacac
     2281 aaatggtgga aaggcatggg gaatcttctg taaaatcagg cctgcatgtt gaggaa
//
LOCUS       DAORYU010000233         2336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_30710_length_2336_cov_4.194213, whole genome shotgun sequence.
ACCESSION   DAORYU010000233 DAORYU010000000
VERSION     DAORYU010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30710_length_2336_cov_4.194213"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1198
                     /gene="murJ"
                     /locus_tag="PLW07_06660"
     CDS             <1..1198
                     /gene="murJ"
                     /locus_tag="PLW07_06660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOL50246.1"
                     /translation="LKYFSLPAKILEILFLLRFTFPYLIFVSLAAWAMGVLNARHRFI
                     LPGLNPIILDFWWIIGLFFFTHFFGNTLDRKIFALLIGVLLGGLSQFVFQLPAVFSVH
                     GPIVLNTRWKHPALIKMLKMFSPVIIGVSVGPINLLVDYSLARSLAEGAVSALWYATR
                     IYQLPLGVFSISLATVLLPHLSGDVAIKKIQQVKDNIQKGLDQTIFLLVPSAIGMIVF
                     RREMIELFFKRGMFTGHSVELTAYPLMLYSIGLVFYGAAIIITRAFYAHHDTATPVKV
                     GLISIGTNALFDMILMKFMGHGGIALSTSLVGIENFVLLIWLFKKKFGLLEMERMTKS
                     FSRVFLMSCFWGIIIEGIRRTLVPYGNSVTVIAGTITGILMYFALAAVLKFPELSGVR
                     ILKWKM"
     gene            1186..1791
                     /locus_tag="PLW07_06665"
     CDS             1186..1791
                     /locus_tag="PLW07_06665"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOL50247.1"
                     /translation="MENVILFFFLIFSVVIHEYSHGLAAYYNGDDTAYLMGRLTLNPV
                     AHLDPIGSILLPLVLALTVGVPFGYAKPVPINPLRFRDYQKGMIMVGAAGCTANFIAG
                     FIFSLISGIAKGMASEIFQLAAFINFILCFFNLIPIPPLDGSRIVAVLLPPRFGELFN
                     RIERYGLFIVYALVFAGGFRFLVPFCSSLVNHFSSLMHLNF"
     gene            1812..2135
                     /locus_tag="PLW07_06670"
     CDS             1812..2135
                     /locus_tag="PLW07_06670"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HOL50248.1"
                     /translation="MEKTDFVELLHFLHVLCWQALGKMVNPVSGKPEKNLQFAKRLID
                     ILETLQMKTKGNLEEDEARMLEDILTDLRLNYVDELNKESKEKESSSQTQDERKTETQ
                     DKNNT"
     gene            2095..>2336
                     /locus_tag="PLW07_06675"
     CDS             2095..>2336
                     /locus_tag="PLW07_06675"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOL50249.1"
                     /translation="MKEKLKHKIKTIPDSPGVYIFKNRSGDAIYVGKSSHLRSRINSY
                     FTTPFEYKRASLLEEIDDIEVIPVASEGEALVLECSL"
BASE COUNT          642 a          391 c          501 g          802 t
ORIGIN      
        1 gctaaagtac ttttctttgc ctgcaaaaat tttagaaata ttgttcttat tgagatttac
       61 attcccgtat ctcatttttg tatctcttgc agcatgggca atgggcgtgc tcaatgcgag
      121 gcacaggttt attctaccag gattaaaccc aattattctt gatttctggt ggataatcgg
      181 cttgttcttt tttactcatt tttttggaaa tacccttgac cggaaaattt tcgctctttt
      241 aataggtgtg ttgctgggtg gattgagcca gtttgttttt caattaccgg cagtgttttc
      301 tgtccatgga ccgattgtgt taaacacccg gtggaaacat cccgctttaa taaaaatgct
      361 gaaaatgttc agtcctgtta ttattggagt gtcggtgggt ccgataaatt tgcttgttga
      421 ctattcgctt gcacgcagcc ttgcagaggg tgcagtatca gcgttgtggt atgcgacaag
      481 gatttatcaa cttccacttg gggttttcag catctctctt gccacagtgc ttttgccgca
      541 tttatcaggg gatgtggcga taaagaagat acagcaggta aaagataaca ttcagaaagg
      601 acttgaccag acaatttttc ttcttgttcc ttctgcgatt ggtatgattg tttttcgaag
      661 agaaatgata gaactttttt tcaaaagagg catgtttaca ggacattctg tggaacttac
      721 cgcgtatcct ttaatgcttt attcaatcgg ccttgtattc tatggtgcag caattattat
      781 cacaagggca ttttatgctc atcacgatac tgcgacacct gtaaaagtag ggcttataag
      841 catcggcaca aatgcgctgt ttgatatgat attgatgaaa tttatggggc acggaggtat
      901 tgcattgtca acatcactgg taggaattga gaattttgtt ttactgatat ggttgtttaa
      961 gaaaaaattt gggttacttg agatggaacg catgacaaaa agtttttcac gtgttttttt
     1021 gatgagttgt ttctggggta taataattga aggaataaga agaacattgg tgccttatgg
     1081 aaattctgta actgtcatag cagggacaat aaccggaata ctaatgtact ttgctcttgc
     1141 cgctgttttg aaatttccgg agctttctgg tgtaaggata ttgaaatgga aaatgtgatt
     1201 ttatttttct ttttgatttt ttctgttgta atccatgaat attcacatgg tctggctgct
     1261 tattataatg gagatgacac tgcatatttg atgggaagat tgacgttaaa ccctgttgct
     1321 catcttgacc cgataggaag tattctgctt cctcttgttc ttgctttaac agtgggagta
     1381 ccttttggat atgcaaaacc tgtgcccatc aatccgctgc gtttcaggga ttaccaaaaa
     1441 ggtatgatta tggtgggtgc tgcgggttgt acggcaaatt ttattgctgg atttattttt
     1501 tctttgatca gcggtatcgc aaaaggaatg gcttctgaaa tatttcaact tgcggcattt
     1561 ataaatttta ttttgtgttt tttcaacctc attccaattc cacctcttga cggttcaaga
     1621 attgttgcag tccttttgcc accaagattt ggtgaattgt ttaacaggat tgaaagatac
     1681 ggtcttttta tcgtttacgc acttgtgttt gctggcggat ttagatttct tgttccattt
     1741 tgttcatccc ttgtgaacca tttttcatca ctcatgcatc ttaattttta gcaaattaac
     1801 ctggagtgaa tatggaaaaa acagattttg tggaactgtt gcattttctt catgtccttt
     1861 gttggcaggc attgggaaag atggtaaacc ctgttagtgg aaaacctgaa aaaaatttac
     1921 agtttgcaaa aaggttgatt gatattcttg aaacactgca gatgaaaaca aagggcaatc
     1981 ttgaagaaga tgaagcaaga atgcttgaag atattcttac tgacctgagg cttaattatg
     2041 tggatgagtt gaataaggag agtaaagaaa aagaatcaag cagtcaaact caagatgaaa
     2101 gaaaaactga aacacaagat aaaaacaata cctgattccc ctggtgtata tatcttcaaa
     2161 aatcgcagtg gagatgcaat ttatgtagga aaatcatcgc accttcgtag ccgaataaat
     2221 tcctatttta ccaccccatt tgaatacaaa agggcctcgt tgcttgagga aattgatgac
     2281 attgaagtca ttcctgttgc atcagaaggc gaagcccttg ttctggaatg ctctct
//
LOCUS       DAORYU010000234         2335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_30726_length_2335_cov_3.784649, whole genome shotgun sequence.
ACCESSION   DAORYU010000234 DAORYU010000000
VERSION     DAORYU010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30726_length_2335_cov_3.784649"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1264)
                     /locus_tag="PLW07_06680"
     CDS             complement(<1..1264)
                     /locus_tag="PLW07_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50250.1"
                     /translation="MKFYKLFLISIFFLSSGVFGQLNNFYIVKNPYNFSIEKVPVVIP
                     VTSTNEKTNFGLYQKKDRKFLLTQQDIVDGGKNEIVGLIDLNPGENRIIEVRTIEKNQ
                     SPEIEGDIKVSYDENKRIYTFVNGFYKFSLSFRKDGRKDWEDIYHVICSDFVYLGNNI
                     ETGIKHISDPQLGVFKGQPEVLMRSGPLRLQLILKGKGELRSGTSRVSGNIHFCITLY
                     SKKPTFDTETIFIPEEESTLYLVAAGETLIIASDKERWNLSSISHQTGKDISIIFGGP
                     EKNSVEGTKWVTAICGDKGFSYVHDDMNSNVSASKGEGGIERSKVVFRLSNFSEKSNY
                     IRPDFFGIRTKKGEPLTLRMRITFYPVVGEEKKFGSNDYMIFQTDFLLPRIVKNTFNF
                     SGDVALSNKDVEKVQQIITSKDVFILAGD"
     gene            complement(1261..2118)
                     /locus_tag="PLW07_06685"
     CDS             complement(1261..2118)
                     /locus_tag="PLW07_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50251.1"
                     /translation="MNIIKNSQSKIQKAAGILFLFFLSSLAVNPAFSDDMPMPRINVY
                     SGRWTWQAKLEYENAYIKCLFRDKNFSPFLKEVFLRQKGENFCMIENCGPSIQKFEKT
                     FVENKGQSFSILCENPVSETTTLPFWKTKGILTFLGNEPRFLIYEEIKILKGATVNFS
                     CFSFDLHAASPTTVYLKTKDKIEKVELKEYRDYCFETNFDFLFVKATTGILGIRINLP
                     VKKNTSIVLPVKGTLFIQHREKTTIGYVTYSAKEVNEGDEIIAGIEFCLFPLSEDIEE
                     IARKMEVKI"
     gene            complement(2128..>2335)
                     /locus_tag="PLW07_06690"
     CDS             complement(2128..>2335)
                     /locus_tag="PLW07_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50252.1"
                     /translation="DSPLDYEKMVPGGVSGQWVVSGGNGDKKMVYPIKVKGLAIEMSR
                     KTLDLTEMVPVRNFSIRLKNLCVE"
BASE COUNT          714 a          463 c          332 g          826 t
ORIGIN      
        1 tatctccagc aagaataaaa acatctttac tggtaataat ttgttgcacc ttttcaacat
       61 ccttgtttga taaagccacg tctcctgaaa aattgaatgt gtttttgaca attcttggca
      121 acaaaaaatc tgtttgaaaa atcatgtagt catttgaacc aaactttttt tcttcaccca
      181 ccactggata gaatgtaatt ctcattctaa gtgttagtgg ttctcccttt tttgtcctga
      241 ttccaaaaaa atcaggtctt atgtaattag atttttcaga aaaatttgat aaacgaaaga
      301 caacttttga acgttctata ccaccttccc ctttgctcgc actgacattg ctgttcatgt
      361 catcatggac atatgaaaat cctttatcgc cgcagatagc agtcacccat tttgtgcctt
      421 ctacgctatt tttttctggt cccccaaaaa ttatggatat gtcctttcct gtttgatgac
      481 ttatagaaga taaattccac ctttctttat cacttgcgat tattaatgtt tcacctgctg
      541 caacaaggta aagtgtgctt tcttcctcgg gtatgaaaat agtttctgta tcaaaagtgg
      601 gtttttttga gtataatgtg atgcaaaaat gaatatttcc tgatactctg gatgtgccac
      661 ttcttaattc tccttttccc ttcaatatca attgaagacg caaaggtcca cttctcatca
      721 aaacttcggg ctgtccctta aagacaccga gctgtggatc agaaatatgt ttgattccgg
      781 tttcaatatt gtttcccaga taaacaaaat cactgcaaat tacatgataa atgtcttccc
      841 agtcctttct tccatccttt ctgaaggata aagaaaactt ataaaaacca ttgacaaatg
      901 tatagattct tttgttttca tcataagaaa ctttaatatc gccttctatt tcaggacttt
      961 ggtttttttc aattgttctg acctcaatga ttctattttc tccaggattc aggtctataa
     1021 gcccaacaat ctcgtttttc ccgccatcta ctatatcctg ctgggtaagt aaaaattttc
     1081 tgtctttctt ctggtacaaa ccaaaatttg tcttctcatt agtactggta acaggtataa
     1141 caactggaac tttttcaata gagaagttat acggattttt cacaatataa aaattattta
     1201 gttgaccaaa tactccacta cttagaaaaa atatgctgat aagaaacagt ttgtaaaatt
     1261 tcatatcttc acctccattt ttcttgcaat ttcttctata tcttcgctta atggaaataa
     1321 acaaaattct ataccagcaa taatttcatc accttcattc acttcctttg cactataggt
     1381 aacataccca atggttgttt tctctctgtg ctggatgaaa agagtaccct ttactggcaa
     1441 aacaatactg gtgtttttct taacaggcag gtttatgcga atacctaaaa ttccagtggt
     1501 tgctttgaca aacaggaaat caaaatttgt ctcaaagcaa tagtccctat actccttcaa
     1561 ttcaactttt tcaattttat cctttgtttt caaatatact gttgttggtg atgcagcatg
     1621 aagatcaaaa gaaaaacagg aaaaattcac tgttgcgcct tttaatatct tgatttcttc
     1681 ataaattaaa aaacgtggct cattacctaa aaaagttaag attccttttg ttttccagaa
     1741 tggaagtgtc gtggtttcgg aaacaggatt ttcacataaa atagagaaag attgtccttt
     1801 attttcaaca aaggtttttt caaatttttg aatagaagga ccacagtttt caatcataca
     1861 aaaattttcg cctttctgtc ttaaaaatac ttcctttaaa aagggagaaa aattcttgtc
     1921 cctgaacaag cattttatat aagcattctc atattccaat tttgcctgcc atgtccacct
     1981 tcctgaataa acattgattc ttggcatggg catatcatct gaaaaagccg ggttaactgc
     2041 aagagacgaa agaaaaaaca aaaaaagtat acctgctgct ttttgtattt tggattgaga
     2101 atttttgata atgttcactg atatctccta ttcaacacaa agatttttta accttattga
     2161 aaaattcctg actggcacca tttcagtaag atcaagtgtt tttcttgaca tttcaatagc
     2221 taatcctttg acctttattg gataaaccat tttcttgtcc ccgtttccac cagacactac
     2281 ccattgacct gataccccgc caggaaccat tttttcatag tccaatggag aatct
//
LOCUS       DAORYU010000235         2332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_30779_length_2332_cov_3.540624, whole genome shotgun sequence.
ACCESSION   DAORYU010000235 DAORYU010000000
VERSION     DAORYU010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30779_length_2332_cov_3.540624"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="PLW07_06695"
     CDS             complement(<1..557)
                     /locus_tag="PLW07_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50253.1"
                     /translation="MKNPESDRKISMKNQVLVVEIDRKNLSVDVRDLRTGILWTMQKT
                     GPCDIGIRGHSGPWQGMPFGSAQQIKWEGNASFQKATLSHWPYRPNVWSALDFAVEVT
                     FQLKDDTLSITVNTRNGRGEASFIDSAYPRGWLFPEKAGGKLLLPYGQGCLLDKNFPV
                     ELDHTFPAYAGMGFVMPWWAQVTDS"
     gene            complement(554..>2332)
                     /locus_tag="PLW07_06700"
     CDS             complement(554..>2332)
                     /locus_tag="PLW07_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50254.1"
                     /translation="IDFVSSVKKYHGNVVNMWPPRNIQVPRGKGTYESDVLWQSQYDK
                     WSLQENALKEIADAFHANGIKLFTMDRAVYPKKLEEFPESPTKDKPAPYISRHSREFL
                     KGFVIEQVKNGVDGVGIGYDEQMGGFRNPANIDEPTKEAFQKKYGIAVPEKAEDSDAF
                     RKWIVFGYEQFASYLAEAAAAAKATNPKVFTKTPVHVCLGTLWNRRIDLNIAEDIVGS
                     TANIDFFRANCYEDYSNLNHYLTAANTKRMVGANRGRAPDSLHNCPWANDPVKYPGYY
                     LDTSPAWMYGPPVISFMHGGGLPLYWRYNFIFYGGYDRYVEQAYSILDTLAAWDAKQA
                     KIPAQILVLKSRASEDWWQVKQRYNPEGNPANQARGFLYEKWLLEFLLSHGYPFKICY
                     LEQIKDFSKELAGYPLIILPFPYSVSQEAFQKIESAVKTGTRIVLMDRQAETDEWGNP
                     YQKPLFSDMVSSGRAMLIKDDVLKVGNDPDFLTDFQNQVDALLGKNKMFFFKNYGNDV
                     ETAVLEKGAKEKFVLFLNWTDRPVQVEAGLKLPDGNYECFQRDLNEVRRVMIGSETKI
                     TARNLQRFLVSLQPWEMKILYIKGVQ"
BASE COUNT          605 a          556 c          448 g          723 t
ORIGIN      
        1 tgcgaatcag tcacctgtgc ccaccatggc ataacaaatc ccattcctgc ataggcaggg
       61 aatgtgtggt caagttccac tggaaagttt ttgtcaagca gacaaccctg tccgtacggc
      121 aataaaagtt tgcctcctgc tttttctgga aacagccatc ccctgggata tgcactatca
      181 ataaacgatg cttctccgcg tccattccgg gtgttaacag taatgcttaa tgtatcatcc
      241 ttaagttgaa aggtcacctc aacagcaaaa tcaagagcac tccatacatt tggtctatat
      301 ggccagtggg aaagggttgc tttttgaaat gaagcgttcc cttcccattt aatttgttga
      361 gccgaaccaa aaggcattcc ctgccagggt ccagaatgtc cccttatgcc tatatcgcat
      421 ggtcctgttt tttgcattgt ccatagaata cctgttcgta aatcacgcac gtccacagaa
      481 aggtttttcc tgtctatttc aactacaagc acctgatttt tcatgctgat tttcctgtca
      541 ctttcagggt ttttcattga actcctttga tataaagaat tttcatttcc catggttgaa
      601 gtgaaacgag aaatcgctgt aaatttctgg ctgtaatttt tgtttctgaa cctatcatca
      661 ccctgcggac ttcattcaga tcccgttgaa aacactcata gtttccatca ggaagtttca
      721 accctgcttc tacctggaca ggtctatctg tccagttcag aaacaaaaca aatttttctt
      781 tcgcaccctt ttcaagaaca gcagtttcaa catcattgcc ataatttttg aaaaagaaca
      841 ttttgttttt tccaagcagt gcatcaactt gattctggaa atctgtcaga aagtcaggat
      901 cattgccgac ttttaacaca tcatccttta ttaacatagc ccttcctgaa gataccatat
      961 ccgaaaaaag cggtttttga tacgggtttc cccactcatc tgtctcagcc tgcctgtcca
     1021 taagaacaat tcttgtccct gtttttactg ctgattcaat cttctggaat gcttcctggc
     1081 tcacactgta agggaagggc aggatgatca gtgggtaccc agccaattct ttcgaaaaat
     1141 cctttatctg ttcaagatag catattttga aggggtagcc gtgactgagt aaaaactcaa
     1201 gtaaccattt ttcatagaga aaaccacgcg cctggtttgc tggatttccc tcaggattgt
     1261 aacgttgctt gacctgccac cagtcctcag acgcccttga ttttaaaaca agtatctgtg
     1321 cagggatttt tgcctgtttt gcgtcccatg ctgccagtgt gtcaagtatt gaatacgcct
     1381 gttccacata cctgtcatag ccaccataga aaataaaatt gtatctccag tagagtggaa
     1441 gtccaccgcc gtgcatgaat gaaataacag gcggtccata catccatgca ggacttgtat
     1501 caaggtaata accaggatat ttcacagggt catttgccca cggacagtta tggagagaat
     1561 caggagccct gcctctattg gcacccacca tccttttggt attggctgct gtgaggtaat
     1621 gattcagatt tgagtagtcc tcatagcagt tagcacggaa gaaatcaata tttgcagtac
     1681 ttccaacaat gtcttctgcg atgtttaggt caatccttct gttccacagt gtcccgagac
     1741 agacgtgtac aggtgttttc gtaaaaacct ttgggtttgt tgcctttgct gcagccgcag
     1801 cctcagcaag atacgaagca aactgttcat atccgaaaac aatccatttc ctgaaggcat
     1861 cactgtcttc tgctttttca gggacagcaa taccatattt tttctggaac gcttcctttg
     1921 tgggttcgtc aatgtttgcc gggttacgaa aaccacccat ctgttcgtca taaccaatac
     1981 ccactccatc aacaccattt tttacctgtt caataacaaa tcctttaaga aactcccggc
     2041 tgtgtctgga gatataagga gctggtttgt cttttgtcgg actttctgga aattcttcaa
     2101 gtttctttgg atagaccgcc ctgtccatgg taaaaagttt gatgccgttt gcgtgaaaag
     2161 catcagcaat ttctttcagg gcattttcct gtaaggacca tttatcatac tgactctgcc
     2221 acagcacatc tgattcgtat gttccttttc ctcttggcac ctgaatgttt cttggtggcc
     2281 acatgttcac cacattgccg tgatattttt tgacactgga cacaaagtca at
//
LOCUS       DAORYU010000236         2317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_31056_length_2317_cov_4.073828, whole genome shotgun sequence.
ACCESSION   DAORYU010000236 DAORYU010000000
VERSION     DAORYU010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31056_length_2317_cov_4.073828"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PLW07_06705"
     CDS             complement(<1..283)
                     /locus_tag="PLW07_06705"
                     /inference="COORDINATES: protein motif:HMM:NF013663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOL50255.1"
                     /translation="MKMSNISIIENPKVPDISGIRNNLEKIVQKMGAKIVPCCKNTEI
                     LISLGGDGTVLKGFRMLPSFDIPLLGLNFGKFGFLTIDCKDMERVVQDVF"
     gene            complement(270..2096)
                     /gene="dxs"
                     /locus_tag="PLW07_06710"
     CDS             complement(270..2096)
                     /gene="dxs"
                     /locus_tag="PLW07_06710"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOL50256.1"
                     /translation="MSALLEKINQSPGTIKQFSRQQLEQLSQEIRNEIIRVVSSNGGH
                     LASNLGIVEITVALHYILDIPPDRVIFDVGHQIYPHKLLTGRFSRFTTLRQYNGISGF
                     PNPAESETDQFLTGHAGTAISSALGLQTIEERNKGKTKTVVVIGDGSLTNGVTFEGLN
                     NLGSSGKDILVILNDNNFSISRTRGALSYYLTKLVTMPVLTKSREEIKEIIEKIPAIG
                     EQIIKFSRDLEQKTKHLIIPGVFFEKLGIQYFGPIDGNDINQIVDVLPNLLNQQGPKI
                     LHAITRKGKGYIPAEKNPEKYHSIGPFNMETGEPLVKIASTGTFVGKSLEKYGEEHAF
                     YVITSAMEYGLGFDGFAKKFPDRFFDVGIAESHSMIFAAGMAKTGKKVFVGIYSTFLQ
                     RTYDQIFHDICLQNLPVVILVDRAGIVSGDGPTHQGIYDLAFLRSIPGITIFCPYSLR
                     NAEEIVKQSLEYKTPVAIRYPRDLLPENVPEIKNNGKILCLGTGSMASIAYEACMSLN
                     QKNIQIDFMPVSKVWPPDNNVENMLSLYKKIATCEEAILTGGFGSSILEICNQKKLSI
                     KVLRIGIPDIFIETGSREALLKNFNLDKENIIEKVKKFYENV"
     gene            complement(2093..>2317)
                     /locus_tag="PLW07_06715"
     CDS             complement(2093..>2317)
                     /locus_tag="PLW07_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50257.1"
                     /translation="YKKNNYKLAEKYLLEAQKKEFDPDIFEHLGYLYYKIKNPVKAIY
                     WWARAQELSPKNEISNMIDTARQQLLINKK"
BASE COUNT          649 a          444 c          398 g          826 t
ORIGIN      
        1 aaaacacgtc ctgtaccact ctctccatat ctttgcaatc tatggtaaga aaaccaaact
       61 ttccgaagtt cagtccgaga agtgggatat caaaagatgg caacatcctg aatcctttta
      121 gaacagtacc gtctccaccg agtgaaatga gaatctctgt atttttacag caaggaacaa
      181 ttttagcacc cattttttga acaatttttt caagattgtt tcttattcca gatatgtctg
      241 gaacctttgg attttcaatg atagagatat tagacatttt cataaaattt tttcaccttt
      301 tcaatgatat tttctttgtc gagattaaaa tttttcagca gtgcttccct tgaacctgtt
      361 tcaatgaata tatcagggat tcctatccgc aaaaccttaa tggataattt tttctgatta
      421 catatttcaa gtatcgacga accaaaacca ccggtaagta ttgcttcttc acaggttgca
      481 atttttttat acaatgataa catgttttcc acattattat ctggtggcca taccttgctt
      541 acaggcataa aatctatttg aatattcttc tggttcaaag acatacatgc ttcataggca
      601 atagatgcca tggaaccagt acccagacaa agaattttac cattattttt tatttctggc
      661 acattttctg gtaataaatc tcgtggatat ctgattgcga ctggcgtttt gtactcaagg
      721 gattgtttaa cgatttcttc agcatttctg agagagtatg gacaaaatat tgtaataccg
      781 ggaatacttc tgagaaatgc caggtcataa ataccctgat gggttggacc atctccagat
      841 acaattccgg ctctgtcaac aagtattaca accggtaagt tctggaggca aatatcatga
      901 aatatctggt catatgtcct ctgtaagaaa gtgctgtaaa ttccgacaaa aacttttttc
      961 cctgttttcg ccattcctgc agcaaaaatc atgctgtggc tttctgcaat acctacatca
     1021 aagaatctgt caggaaattt ttttgcgaat ccatcaaatc caagaccata ttccatggca
     1081 gatgtaatga catagaaggc atgttcttct ccatattttt cgagagattt tccaacaaag
     1141 gttcctgtgg atgcaatttt tacaaggggt tcgcctgttt ccatattaaa agggccgata
     1201 ctgtgatact tttctgggtt tttctcagca ggaatatatc cctttccttt tcttgttatc
     1261 gcatgtaata tttttggacc ttgttgatta agaagattcg gcagaacatc tacaatttga
     1321 ttgatatcat ttccgtcgat gggtccaaaa tattgaatac caagtttttc aaaaaatact
     1381 cctggaataa tcagatgttt tgtcttttgc tccaggtctc tgctgaattt tattatttgc
     1441 tcacctatcg caggtatttt ttcaataatt tcctttattt cttctctgga ttttgtcagt
     1501 acaggcatgg taacaagttt tgtcagataa taagataaag ccccgcgggt tcttgatatt
     1561 gaaaaattgt tatcattgag tataacaaga atatctttcc cggatgaacc caaattattc
     1621 agtccttcaa atgttacgcc atttgtcagg ctgccatctc caattacaac cactgttttt
     1681 gttttccctt tattcctttc ttcaatggtc tgaagaccca gagctgatga gattgctgtg
     1741 ccagcgtgtc ctgtcaaaaa ctggtctgtt tcgctttctg ccggatttgg aaaacctgaa
     1801 attccattgt attgtctgag ggtggtaaat ctactgaacc ttcctgtaag taatttgtgt
     1861 ggatatatct ggtgtccaac atcaaatatt actctatctg gcggaatatc cagaatataa
     1921 tgcaatgcaa cagtaatttc cacgattcca agatttgagg caagatgacc accgttactg
     1981 gagacaaccc ttataatttc attccttatt tcctgagaca gttgttccag ttgttgcctg
     2041 gaaaactgct ttatggtgcc gggtgattga ttgatttttt caagaagtgc gctcatttct
     2101 tgttgatcag taactgctgt cttgccgtat caatcatatt tgatatttca tttttgggag
     2161 acaattcttg agctcttgcc caccagtaaa ttgctttaac tggatttttt attttgtaat
     2221 aaaggtatcc aagatgttca aaaatatctg gatcaaattc ttttttttga gcttccaata
     2281 aatatttttc tgcaagttta tagttgtttt ttttata
//
LOCUS       DAORYU010000237         2314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_31117_length_2314_cov_2.868526, whole genome shotgun sequence.
ACCESSION   DAORYU010000237 DAORYU010000000
VERSION     DAORYU010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31117_length_2314_cov_2.868526"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PLW07_06720"
     CDS             <1..224
                     /locus_tag="PLW07_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50258.1"
                     /translation="NYTIDLPQDTQILYPILIDVNEPQINVKVDLKLSTAGRELTTMI
                     GYIVVKKIKGEGWKVIDFKVDDAGKMKKM"
     gene            491..1549
                     /locus_tag="PLW07_06725"
     CDS             491..1549
                     /locus_tag="PLW07_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOL50259.1"
                     /translation="MSHDSMNPKERWTAVLQRKKPDRIPLFWSSTQETLDKMKKFLGV
                     SENSEFFKKLHVDEIVSVFPAYVGPRVSEGYDFFGCGYKKVSYGTGSYSECVYHPLAG
                     YETLSEIKSNYKFPSIDWFDCSVIKKQIQGKEHLPIYGGGMEPFLRYTQLRGLEKAMM
                     DLVEKPDIVEYCLENIFQLYYRITMGIYEEIPGKVLLTMVAEDLGSQETLLFSPETIR
                     KFFLPRMKKMMSLAHENGACVITHSDGAIRDIVPDLIECGMDVLNPVQWRCRGMDREK
                     LKKDFGNRICFHGAMDNQHTMPFGTVEDVKQEVMDNIRILGENGGYFLGPCHNLQAIT
                     PPENILAMYDTAYENGWY"
     gene            1552..2160
                     /locus_tag="PLW07_06730"
     CDS             1552..2160
                     /locus_tag="PLW07_06730"
                     /inference="COORDINATES: protein motif:HMM:NF024747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HOL50260.1"
                     /translation="MLEICEIFNSIQGEGWRQGIPAVFIRFSGCNLRCKFCDTPQAFT
                     EKNEMTEETIVKTVKRLAKKYPGITNVVITGGEPYLQDFSMLVGMLKKAKFFVAVETN
                     GTIWRKIPADWICVSPKLQAKKILKNGYDERFKHIASEFKYVITRRADFAFIDKEISQ
                     PVILQPVNNNLKIARMICNEMKKNGMPNWFIRLQQHKLLEIK"
BASE COUNT          770 a          345 c          511 g          688 t
ORIGIN      
        1 acaactatac tatagattta ccgcaggata ctcaaatttt atatcccatt ttaatagatg
       61 taaatgaacc tcaaataaat gtaaaagtag acctcaaact ttctactgcg ggaagagaac
      121 ttacaactat gataggatat atcgttgtga aaaagataaa aggcgaagga tggaaagtta
      181 ttgattttaa ggtggatgac gcaggtaaaa tgaaaaaaat gtaatacaga aaaacttata
      241 atacgaagag aatattatcg atttgatgtt ttgtctattt ggtttgttat acaaaaatcc
      301 gtaaggttat aaatccaaat tgttgttatc agaagtggtt attaaaccga ttcaagtaca
      361 agcatttagt cagtacaaca gaatgatttt tcccagaaga ttacattcag aaaatcaaat
      421 aagtccaaaa ccagccattt gagtcatttc tactcaacgg gtataatatt tcaatttaac
      481 agggggaaaa atgtcgcatg attcaatgaa tccaaaagaa cgctggactg cagtattaca
      541 gagaaaaaaa cctgacagga ttccgctttt ctggtcatcc actcaggaaa cccttgataa
      601 aatgaaaaaa tttctcggag ttagcgagaa ttctgagttt tttaagaaac ttcatgttga
      661 tgagattgta agtgtttttc ctgcctatgt tggacctcga gtttctgagg gctatgattt
      721 ttttggctgt ggatataaga aagtttccta tgggacaggt agttattctg aatgcgtgta
      781 tcatcctctg gcaggatatg aaacactttc tgaaataaaa agtaattata aatttccttc
      841 aattgactgg tttgattgtt cagtgattaa aaaacagatt cagggcaaag aacatcttcc
      901 gatttatggc ggcggcatgg aacctttttt gagatacaca caattgcgtg gtcttgaaaa
      961 ggcgatgatg gatcttgttg aaaaacctga tattgttgag tactgccttg agaatatctt
     1021 tcaactttat tacaggatta caatgggtat ctacgaagaa atacctggaa aggtgcttct
     1081 aacaatggtt gcagaggacc ttggttcaca ggagacgctg ctgttttcgc ctgaaacgat
     1141 aagaaagttt tttcttccca ggatgaaaaa aatgatgtcg cttgcgcatg aaaatggagc
     1201 atgtgtgatc actcacagcg atggtgcaat cagggatatt gtgccggatt tgattgaatg
     1261 tggaatggat gttcttaatc ctgtccagtg gcgatgcagg ggaatggaca gggaaaaatt
     1321 gaaaaaggat tttggcaaca gaatctgttt tcacggcgca atggacaatc aacataccat
     1381 gccttttgga actgttgagg atgtgaagca ggaggttatg gataacatca gaattcttgg
     1441 tgaaaacggt ggatattttt tgggcccatg tcacaacctg caggctatta ccccacctga
     1501 aaatatttta gcaatgtatg ataccgcgta tgagaatggc tggtattgac gatgcttgaa
     1561 atctgcgaga tcttcaattc aatacagggt gaaggatgga ggcagggaat tcctgcggtt
     1621 ttcataagat tttctggctg taatctcagg tgcaaattct gtgatacacc acaggcattc
     1681 acagagaaaa atgagatgac agaagaaact attgttaaga cagtaaagag attggctaaa
     1741 aaatatccag gtattaccaa tgttgtgatt actggagggg agccatatct tcaggatttt
     1801 tcaatgcttg ttgggatgtt gaaaaaagca aaattttttg tggcagttga aaccaatggt
     1861 actatctgga gaaaaattcc tgctgactgg atttgtgtta gtcccaagtt gcaggcaaaa
     1921 aagattttga aaaatggcta tgacgaaaga ttcaaacata ttgccagtga atttaagtat
     1981 gttattacac gcagagcaga ttttgctttt atagataaag agatttctca accagtgatt
     2041 ttgcaaccag taaataataa tttgaaaatt gccagaatga tttgcaatga gatgaaaaaa
     2101 aatggtatgc caaactggtt tataagatta caacaacata aactattgga aataaaatga
     2161 aatcagtggc tctcatatca ggtggaattg atagttttgt tgctgctgct tttgaaaagg
     2221 cacagggtag tgatatctat ggattgagcg ttaattacgg tcagatccac agaaaagaac
     2281 ttgtttgcgc aaggaaaatt gggcggtttc ttgg
//
LOCUS       DAORYU010000238         2300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_31353_length_2300_cov_3.727394, whole genome shotgun sequence.
ACCESSION   DAORYU010000238 DAORYU010000000
VERSION     DAORYU010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31353_length_2300_cov_3.727394"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..2142
                     /locus_tag="PLW07_06735"
     CDS             70..2142
                     /locus_tag="PLW07_06735"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOL50261.1"
                     /translation="MIELLDGISCLPFYLSVIKCNSKTSMQSRKGLIIAVILTAIIQV
                     PAYTMDKENIRNIYVATDGNDQWSGLYPQALPDRNDGHLATIEAARDKIRKLKSSGLF
                     SGPITVWIRGGRYHLTQPIVFTHQDSGPITYRNYPGEKVILDCGVKISGWKKTQVNGV
                     DAWVTEIPEVKQGKWYFRQLFVNGKRKNRTRLPETGYFRMKDFPDSDKKTAFFDGSDC
                     FYCSPGDIKQWRNLKDIDIVVFHWWIEERMPVEYFDENTGLVKSSRKSKMVLKDENDG
                     KPARYWVENVFEQLKTPSQWYLDRKQGLLYYIPENNETIETSEIYAPVLTQAIIIQGN
                     SENNQYVEYLTFKGLTIEHADWQMPDDGFASSEQAARKIPGVFYLEGVRFYTIEECKI
                     QHCGFYGIEIAEGCSGIRIHKNNLEDLGAGGVKINGGDAYQPDYTRTKNITVTENHIH
                     HCGNVFYSAVGILSMHASGNVISSNHIHDLYYSGISCGWVWGYGENVSKNNLIENNII
                     HDIGKGLLSDMGGIYTLGVQPGTVIRGNIIYRIEKYSYGGWCIYLDEGSSHIIVENNL
                     CAFTNHHVFHIHYGRENIVRNNIFMFGEEGIVAFSKKEQHCSFTFERNILISKGKPFF
                     NGGYVETLEVFLGDLNILYDVSVKEIYWGTIEGNNISFSQAQHIGHDVHSIVADPGFA
                     NPSSLILH"
BASE COUNT          795 a          391 c          479 g          635 t
ORIGIN      
        1 aatcctcaag tgcaagaatg attttttgaa attccatgca aataattata acataaatca
       61 gaattatcaa tgattgaact ccttgatgga atttcttgtt tgccttttta tctgtctgtg
      121 ataaaatgca attccaaaac atccatgcaa tcgagaaaag gattgattat agcggttata
      181 ctgacagcaa ttatacaggt acccgcttat acgatggata aagaaaatat cagaaatatt
      241 tatgtagcaa cagatggaaa tgatcagtgg tcaggattgt atccacaggc actgcctgac
      301 agaaacgatg gacacctggc aacaattgaa gcagcaaggg acaaaatcag gaaattaaaa
      361 tccagcggtt tattttcagg tccaattact gtctggattc gtggtggaag atatcacctt
      421 acgcagccca ttgtttttac ccaccaggat tcaggaccaa tcacgtatag aaactatcct
      481 ggagaaaaag tgatccttga ttgcggggta aaaatttcag gatggaaaaa aacacaggtc
      541 aatggtgttg acgcatgggt tacagaaata ccagaagtaa aacagggaaa atggtatttc
      601 agacaactgt ttgtcaatgg gaaacgaaaa aatagaacca gattaccgga aaccggatat
      661 tttagaatga aggatttccc ggattcagat aaaaaaacag ctttttttga cggaagcgat
      721 tgtttttact gcagtccagg agatatcaaa caatggagaa atctcaaaga tattgacata
      781 gtagtgtttc actggtggat tgaagagaga atgcctgttg aatactttga tgaaaacaca
      841 ggacttgtga aatcatcaag aaaaagcaaa atggtgctga aagacgagaa tgacggcaag
      901 cctgcaagat actgggttga aaatgtcttt gaacaattga aaacaccatc ccagtggtac
      961 cttgatagaa agcagggtct tctgtattac atccctgaaa acaatgaaac aattgaaaca
     1021 tctgaaattt atgctcctgt gctcacgcag gcaataataa ttcaaggaaa tagtgaaaac
     1081 aatcaatatg tagaatatct aacattcaaa ggtctcacca ttgaacatgc tgactggcaa
     1141 atgcctgatg atggattcgc atcatctgaa caggcagcca ggaaaatacc tggtgttttc
     1201 tatttagaag gagtacgctt ctacaccatt gaagaatgca aaatacagca ctgcggattt
     1261 tatggtattg aaattgcaga aggttgttct ggcattagaa ttcataaaaa caatcttgaa
     1321 gacctcggag caggtggagt taaaatcaat ggtggagatg catatcaacc agattatacc
     1381 agaacaaaaa acatcacagt cactgaaaat catattcatc actgtggaaa tgtgttctac
     1441 agtgctgttg gcatactttc aatgcatgca tcaggtaatg tcatttcctc aaaccacatt
     1501 catgaccttt attattcagg aatttcatgc ggctgggtat ggggctatgg tgaaaatgtt
     1561 tcaaaaaaca accttattga aaacaatatc attcatgaca taggtaaggg tcttctcagt
     1621 gatatgggtg ggatatacac gcttggggtt caaccaggca cagtcatcag gggcaatatc
     1681 atctacagaa ttgaaaaata ttcatacggt ggctggtgca tatatctaga tgaaggtagt
     1741 tctcacatta ttgttgagaa caatctctgc gctttcacaa atcaccatgt ttttcacatt
     1801 cactatggca gggaaaatat tgtgagaaac aacatcttta tgtttggaga agaaggcatt
     1861 gttgcctttt caaaaaagga gcaacattgt tcgtttactt ttgaaagaaa tatccttatc
     1921 tcaaaaggaa aaccgttttt caatggtggt tatgtggaaa cacttgaagt atttttgggt
     1981 gatttgaata ttctgtacga tgtctcagtc aaagaaatct actggggaac tattgaagga
     2041 aacaatatct cattttccca agcacaacat atcggacatg atgttcattc aattgtcgct
     2101 gacccaggtt ttgctaatcc gagcagtttg attttacact gaaatccgac gaggtaataa
     2161 aaaaaattgg atttaaacct ttcataacaa agaaactgga gatgatgaat gaatgatgtt
     2221 ccgctcatat taagacgcgc agacgattca ggtgcaaaga tatgtgtggt cagtgttcca
     2281 ttgagggaga aagaccagca
//
LOCUS       DAORYU010000239         2289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_31564_length_2289_cov_7.583706, whole genome shotgun sequence.
ACCESSION   DAORYU010000239 DAORYU010000000
VERSION     DAORYU010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31564_length_2289_cov_7.583706"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PLW07_06740"
     CDS             complement(<1..190)
                     /locus_tag="PLW07_06740"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOL50262.1"
                     /translation="MEDEINLIDLWQVVWKYRWMTVLLTMIAVVVTGIVSLNLPKQYK
                     ATASVLPQIEMSKVQMAVP"
     gene            complement(207..>2289)
                     /locus_tag="PLW07_06745"
     CDS             complement(207..>2289)
                     /locus_tag="PLW07_06745"
                     /inference="COORDINATES: protein motif:HMM:NF018010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOL50263.1"
                     /translation="VPPIGPVGAIAGYVKRPGVYELSGKTKISDLIAIAGGMIPTAYI
                     NRLQVERIKEHEKRILLDLEFKDVSELEHSANNLVLQDGDLVLIYPILPYKRGWVAIE
                     GNVTRPGEYQFVEGMSISDLIKKAEGVLPEFYERAEILRFVSEHTRQPITFNLEKLLK
                     GDKNEDLPLKEWDIVKIYSLYDIYPEPIVEIRGNVHKPGAYRFIEGMKVSDLLFRGEN
                     PTVEAYLERAELYRIRKDKTREMKVINLKEILDGKKEADITLEPWDKLVVYSLYDIFP
                     TGYVGVEGAVQKPGNYELTQGMKISDLLFQAGYPLKEAYLERAELYRFRKDKTREMIP
                     INLNKVLSGDEKENMALEEKDMLKIYTISEVIPKSYVEISGAVYEPGRYELTTNMKIS
                     DLIFQAKGLKKFASPIAELYRQRPGDTPEVIKLDITKDDIVLHEDDHLFIRYETEWIE
                     KNKITISGEVVKPGEYVIQKGETLVDILKRAGGFTDKAYLPAAIFTRQSVKELQKKHM
                     MEFIEMEQRKILEEESSLTTLNLTEVERKQRMDSLQYRKGILESLSKVDVPGRIIINL
                     PKILEGNEKLLVENGDTLHIPQIPSSVQIIGAVYNPGAIFYEPGKDINYYLAKVGGPT
                     KQANVKEIYVIRASGEVLSDIKKFGTRIERGDTIVVPQLYTFKTPAGILLRDIAQVFY
                     QFALPIAAIIK"
BASE COUNT          624 a          496 c          344 g          825 t
ORIGIN      
        1 caggtacagc catctgaacc tttgacattt caatctgggg taaaaccgaa gcagtagctt
       61 tatactgttt tggtaaattc aaactcacaa ttccagtaac aacaactgcg atcattgtca
      121 aaagtactgt catccatcga tacttccaca caacttgcca cagatcaatc agattaattt
      181 catcttccat ttgacttatc ctgttgtcac tttattattg ctgcaatagg caatgcaaat
      241 tgataaaaca cctgggcaat gtcccttaat aaaatgcctg ccggagtttt aaatgtgtat
      301 agctgaggaa caacaattgt atcgcctcgt tctattcttg ttccaaattt ctttatatct
      361 gataaaacct ctccactggc tctgattaca taaatttcct tgacatttgc ctgttttgtt
      421 ggaccaccta cttttgcgag ataataattt atatcttttc ccggttcata aaatatagca
      481 ccaggattat aaactgctcc gataatttga actgaacttg gaatttgcgg aatgtgtaag
      541 gtgtcaccgt tttcaacaag caacttttca tttccttcca gaatttttgg cagatttatg
      601 ataattcttc caggaacatc aaccttggaa agtgattcaa gaattccctt cctgtattgc
      661 agtgaatcca ttctttgttt tctctctact tcagtaagat tcaaagtagt taaacttgat
      721 tcttcttcaa gaatttttct ttgttccatt tcaataaatt ccatcatatg tttcttttga
      781 agttctttta cactctgcct tgtaaaaata gctgctggaa ggtatgcctt atcagtaaaa
      841 ccacctgctc gttttaaaat atcaaccaag gtttcacctt tttgaataac atattcgcca
      901 ggttttacca cttctcctga aatcgtgatt ttgttttttt caatccattc tgtttcatac
      961 ctaataaaaa gatggtcatc ttcatgaagg acaatgtcat ctttagtgat atcaagtttg
     1021 attacttctg gcgtatcgcc tggtcgctgt ctgtaaagct ctgcaatcgg tgatgcgaat
     1081 ttttttaatc cctttgcctg gaaaatcagg tcactgatct tcatattggt tgtcaactca
     1141 tatctgcctg gttcatacac agctccactg atttctacat aacttttcgg aattacctca
     1201 gaaattgtat aaatttttaa catatccttt tcttcaagcg ccatattttc cttttcatct
     1261 ccgctgagaa ctttattaag attgatgggt atcatttctc tggttttatc ctttctgaac
     1321 ctatatagtt ctgcccgctc aagatatgct tctttaagag gataacctgc ctgaaatagc
     1381 aaatcagata ttttcattcc ttgtgttagt tcatagttcc ctggtttttg cactgcacct
     1441 tctactccta cataaccggt cggaaaaata tcgtataacg aatatacgac cagtttatcc
     1501 cagggctcca gcgtaatatc tgcttccttt tttccatcaa gtatttcctt caggttaatg
     1561 accttcattt cgcgtgtttt atcctttctg attcggtaaa gttctgcccg ctcaaggtat
     1621 gcttcaacag ttgggttttc accgcgaaag agcaaatcac ttaccttcat gccttcaata
     1681 aacctgtaag cacctggttt atgtacattt cctctaattt ccacaattgg ttcaggataa
     1741 atgtcataga gtgaataaat ttttacaatg tcccattctt ttaatggcaa atcttcattt
     1801 ttatcgcctt tcaaaagttt ttcaaggtta aaagtaatag gttgccttgt atgttcagag
     1861 acaaaacgca atatctcagc cctttcataa aattctggaa gtactccttc tgctttttta
     1921 attaaatctg aaatgctcat tccctcaaca aattgatatt ctccgggtct tgtcacatta
     1981 ccttctattg caacccaacc ccgtttgtat ggcaatattg ggtatattaa aactaaatct
     2041 ccatcctgca aaacaagatt gtttgctgaa tgttcaagtt ctgaaacatc cttaaattca
     2101 aggtcaagaa gtattcgttt ttcatgttcc tttattcttt caacctgcaa tctatttata
     2161 tatgcagtcg gaatcattcc tcctgcaatt gcaatgaggt cactgatttt tgtttttcct
     2221 gaaagttcgt aaacgccagg cctttttaca tatcccgcaa tagcacccac aggtcctatc
     2281 ggtggaaca
//
LOCUS       DAORYU010000240         2272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_31899_length_2272_cov_3.692377, whole genome shotgun sequence.
ACCESSION   DAORYU010000240 DAORYU010000000
VERSION     DAORYU010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31899_length_2272_cov_3.692377"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2213)
                     /locus_tag="PLW07_06750"
     CDS             complement(<1..2213)
                     /locus_tag="PLW07_06750"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOL50264.1"
                     /translation="MKNHIFLKAVNPCKEPATFGLNASIPFPKGLLKTTESLILYDYD
                     NSVIPCQKKVLARWNDKSIKVVLIDFVATLKSGTNKYRISFEGNKETENIPQGSLKIR
                     SGSDGDENVAIIDNKFSLKLTLVDSKNLKYHAKIKKVKKQYGKIKKVTIIYGSFSTKT
                     NLRGIDFVLTLTTWTGSNLVKVEPEIHCNPEKGVFFRIKEFKLSITPISNNIAEAFFP
                     GVQINQTKKIKILQVDDNCDIETDENLEKRHIASTGTFRVAANGLIIAGALKDFSMRW
                     PKSMEFDGHSISFGLFPSFKKGAFNHMQPLYKYGYLFYGSTYMLKTGQKRRWEIWLDM
                     DGNWQLCRSMCEIPPMAVVEPVYAVSTGVYGDIPAAGDNTRDYDRVFSNMFNLYYSSI
                     RKNRDTGEMNWGDWFGERYIHWGNNEYDTAEQLFVQFVRTGDLRYFVSGENAARHTEE
                     VDIIHYVNKDLIDSFNEEPFRPFDVVPGCVHQHTIGHVGGFFSRKKAQKMVSRALGIK
                     NPYPCTEPWNMGHLWLEGLRDAYFLTGDERFKQTVILVADYLSKIVIEKLYPFTGHAH
                     SGRTVGWPLTALCAAYDIKKDKNYLKAMKILVNDTLAEQDPHCGGWLYEMPDGHCNCI
                     RKKHVGTAGFLISILVNGLSRYYEITGDKKIPQAVKKAVEFLVADTWDERTGKFRLSS
                     CPASPYLQLFSLTLFSLANGIKIGNADVRDVFVKNWKHFLDYVDKEIDERHIGWM"
BASE COUNT          662 a          493 c          364 g          753 t
ORIGIN      
        1 aacatccagc caatatgtct ttcatcgatt tccttatcaa cataatctaa gaaatgcttc
       61 cagtttttta caaaaacatc cctgacatca gcatttccaa tttttatgcc gtttgcaaga
      121 gaaaacaagg tcaaactaaa caattgaaga tatggagaag caggacagga actaagccta
      181 aattttccag ttctttcatc ccatgtatca gcaacaagga attccactgc ttttttcact
      241 gcctgcggaa tttttttatc acctgtaatt tcgtaatacc tgctcaaccc atttacaagg
      301 atgctgatta gaaaaccagc ggtacccaca tgtttttttc tgatgcagtt acaatgacca
      361 tcaggcattt cataaagcca accaccacaa tgagggtcct gttcagcaag tgtatcatta
      421 acaagtatct tcattgcttt gaggtaattt ttgtcttttt taatgtcata tgccgcacag
      481 agcgcagtca aaggccatcc aacagttctg cccgaatgag catgcccggt aaaaggatag
      541 agtttttcta tcacaatttt tgaaaggtaa tcagcaacaa gaatcacagt ttgtttaaat
      601 ctttcatctc ctgtaagaaa atatgcgtct cttaaaccct caagccacag gtgtcccatg
      661 ttccaaggct cagtacaggg ataaggattc tttattccaa gcgccctgct taccatcttt
      721 tgcgcttttt ttcttgaaaa aaatccaccg acatggccta ttgtatgttg gtggacacaa
      781 cctggcacca catcaaatgg tctgaaaggt tcttcattaa atgagtcaat caggtctttg
      841 tttacatagt gaataatatc cacttcctct gtatgtcttg cggcattttc accagacaca
      901 aaatatctca aatctcctgt ccttacaaac tggacaaaaa gttgctctgc tgtgtcatat
      961 tcgttgtttc cccagtgaat atatctttct ccaaaccaat caccccagtt catctctcct
     1021 gtatccctgt ttttcctgat gcttgagtaa tacagattga acatattgga aaacactcta
     1081 tcgtaatctc tggtattgtc tcctgctgca ggaatatctc cgtaaacgcc ggttgaaact
     1141 gcatatactg gttctacaac cgccataggc ggtatttcac acattgatct acacaactgc
     1201 cagtttccat ccatatcaag ccatatttcc catcttcttt tctgacctgt tttcaacata
     1261 tatgtagaac cgtaaaacag gtatccatac ttgtacaatg gttgcatgtg gttaaatgcg
     1321 ccttttttga atgaaggaaa taacccaaaa ctgatggagt gtccatcaaa ttccatactt
     1381 tttggccatc tcattgaaaa gtccttcaaa gcacccgcta taatcagacc attggcggct
     1441 accctgaagg taccagtcga agcaatgtgt cttttttcaa gattttcgtc tgtttcaata
     1501 tcacagttgt cgtcaacctg caaaattttt atcttctttg tttgatttat ctgaacacct
     1561 gggaaaaatg cttctgcaat attatttgaa atcggtgtta tagaaagttt gaactctttg
     1621 atcctgaaaa agacgccttt ttcaggattg caatgaattt caggttctac ttttaccaga
     1681 ttactgcccg tccatgttgt taatgtcaga acaaaatcaa ttccacgcaa atttgttttc
     1741 gtcgaaaatg aaccataaat aattgtaact ttttttattt ttccatattg ttttttcacc
     1801 tttttaattt ttgcatgata tttcaaattc ttactgtcga caagcgtcaa tttcaaggag
     1861 aatttattgt caataatcgc cacattttca tccccatcac tacctgaacg aattttcaat
     1921 gaaccctgtg gaatattttc tgtttcttta ttaccttcaa aagaaattct gtatttgttt
     1981 gtacctgatt taagggtcgc aacaaaatca atgagaacca ctttaatgct tttgtcattc
     2041 catctggcaa gaaccttttt ctggcaggga atcacactat tatcataatc ataaagaatc
     2101 aaggactctg tggtttttag caagcccttt ggaaaaggaa tacttgcatt aagaccaaat
     2161 gtcgcaggtt ccttacatgg attgactgcc ttcaaaaaaa tatggttttt catagtgtct
     2221 cagcatgagt acttgtcatc tggaagatac ggcgttttaa tgaaaacaag tt
//
LOCUS       DAORYU010000241         2259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_32157_length_2259_cov_3.021779, whole genome shotgun sequence.
ACCESSION   DAORYU010000241 DAORYU010000000
VERSION     DAORYU010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32157_length_2259_cov_3.021779"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="PLW07_06755"
     CDS             complement(<1..682)
                     /locus_tag="PLW07_06755"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOL50265.1"
                     /translation="MKRNFLTVLIAGMVLLVFSSGLHAKINVVATLPWIADIAKQIGK
                     DNIAVTTLVKASQDPHYVEAKPSLIIAARKTDILMYNGLDLEIGYLPVIIESSKNPNI
                     QPGKIGNLDCSRFITPIEKPSSVDRSMGDVHPLGNPHYHFSPSNILKVAQGITETLVT
                     IDPSNESYYRKNYQDFYSKWQEKMKQWKGANLAGKKFVAYHTVFSYLAKEFNFQIVGC
                     IEAKPGIPP"
     gene            complement(695..2047)
                     /locus_tag="PLW07_06760"
     CDS             complement(695..2047)
                     /locus_tag="PLW07_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50266.1"
                     /translation="MKINKFIPIHCMLIGLIIIFAPIAKCQDINERIKSLEEMLKKQQ
                     ETIQKQQEIITQLQEELKQIKKQAAGTPVMTEEERPSYISGTLFGASSAYNPNISLIL
                     DTFGYSSSLKKDELEHWNIPGFTSEGIDHQEGLNIHAAELYLFAPVDPFFNLYATIPV
                     TQEGVELEEAYLITTNLPAGLQAKIGKFKTGFGRLNSQHAHVWNFVDQPLVYRAFIGH
                     EGINEKGIQLTWLPALPIYTQFGIEALQGENEVLFGNDATTGLHAFGAFIKSSIDVGP
                     YGTILFGPSIVTGKTKTDSIEKDHVFSGDSTLVGFEFTYKWKPSRWKSFALQSEYIYR
                     TQKGKLQDINTLDVQNLKRSQDGLYLQTNYQTGRWGFGARYDLLEPFNDTYKLDGTNI
                     SFGDKPWRASAMVEFNPSEFSKIRLQYNYDKSSRDGRTNNEWYLQFIGGVGAHAAHTF
                     "
BASE COUNT          634 a          414 c          446 g          765 t
ORIGIN      
        1 aaggcgggat acctggtttt gcttcaatgc aaccaacaat ctggaaattg aattcttttg
       61 caagatagga aaaaacagtg tgatatgcga cgaatttttt tcctgcaagg tttgctccct
      121 tccattgttt cattttttcc tgccattttg aataaaaatc ctggtagttt ttcctgtagt
      181 atgactcatt tgacgggtca attgttacca gtgtttctgt gataccctga gcgactttca
      241 agatattgga aggagaaaaa tgataatggg gattaccgag tggatgtaca tctcccatgc
      301 ttctgtcaac agatgatggt ttttcaatgg gagtgataaa acgagagcaa tctaaattac
      361 cgatttttcc gggttgtata ttgggatttt ttgatgattc tataatcaca ggaaggtacc
      421 caatttcaag gtcaagccca ttatacatca ggatgtctgt ttttctcgct gcaatgatta
      481 aactgggttt tgcttccaca tagtgagggt cctgacttgc tttaacaagt gttgtcactg
      541 caatattgtc cttcccgatt tgttttgcaa tgtctgcaat ccatggtagt gttgcaacaa
      601 cattgatttt tgcatgaaga cctgatgaaa atacaagcaa taccattcca gcaatcagaa
      661 ctgttagaaa atttcttttc atattttccc ccttttagaa agtatgagcc gcatgcgctc
      721 caactccacc aataaattgt aagtaccatt cattgttagt tcttccatcc cgtgaactct
      781 tatcgtaatt gtattgaagg cgtatttttg aaaattcaga agggttgaat tccaccattg
      841 ctgatgctct ccatggtttg tctccaaagg aaatgttggt gccgtcaagt ttgtatgtgt
      901 cgttgaaagg ttcaagaagg tcatatcttg caccaaaccc ccatcgtcct gtttgataat
      961 ttgtttgaag atataaacca tcctgacttc ttttcaaatt ctggacatcc agagtattta
     1021 tatcctgaag ttttcctttc tgggttctgt atatatattc actttgaagt gcaaaacttt
     1081 tccatcttga tggtttccac ttataggtga attcaaagcc cacaagtgtg gagtcgcctg
     1141 agaaaacatg gtctttttca attgaatctg tttttgtttt tcccgtaacg atagatggcc
     1201 caaaaagaat tgttccatat ggaccaacat caattgaaga ttttataaac gcgccaaatg
     1261 catggagtcc agttgttgca tcgtttccaa acaacacttc gttttctccc tgcagtgctt
     1321 caatgccaaa ctgggtgtaa attggtaatg ctggcaacca ggtcaattga attccctttt
     1381 cattgatgcc ttcatgtccg ataaatgctc tgtaaacaag gggttggtca acaaaattcc
     1441 agacatgggc atgctgtgaa ttcaatctac caaatccagt tttgaattta ccaatttttg
     1501 cctgaagacc tgctggaaga tttgttgtaa taagatatgc ttcttcaagt tcaacgcctt
     1561 cttgtgttac tggaattgtg gcatatagat tgaaaaatgg gtcaacagga gcaaaaagat
     1621 atagttctgc tgcatgaatg ttcaatcctt cctggtggtc aataccttct gaagtaaaac
     1681 cagggatatt ccagtgttca agttcgtctt tttttaaact ggaactgtag ccaaaagtat
     1741 caagaatcaa agagatgttt ggattatagg cgcttgatgc tccaaatagt gttccagaaa
     1801 tgtatgatgg tctttcttct tctgtcataa caggtgttcc ggctgcctgt tttttaattt
     1861 gtttgagttc ttcctgaagt tgtgtgatga tttcctgttg cttttgaatt gtttcctgtt
     1921 gtttttttaa catttcttca agggatttta ttctttcatt aatatcctga cattttgcaa
     1981 tcggcgcaaa aatgataatc aggccgatta acatgcaatg tataggaata aatttgttga
     2041 ttttcacagc cacctcctgt ttgaatgaac atcacatctt gggtacgacc taaattaaac
     2101 aaaatttcag gagggtttta acagggtggt ccacgggtga gaaaatttga aggatgggtc
     2161 tctcttttaa aggaaacttt tttgtatgat acagcccagg aaataaagat aatgaaaaga
     2221 gataaaaaca actgaaatga aaatggagca aaggcgctc
//
LOCUS       DAORYU010000242         2214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_33013_length_2214_cov_3.338583, whole genome shotgun sequence.
ACCESSION   DAORYU010000242 DAORYU010000000
VERSION     DAORYU010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33013_length_2214_cov_3.338583"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1282
                     /locus_tag="PLW07_06765"
     CDS             <1..1282
                     /locus_tag="PLW07_06765"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOL50267.1"
                     /translation="IMCSVSAIAGMFTKEIAIFFPFLILMYFFWFRKKLNCVIKTWEV
                     IFYSVFLFSIIPAILLLTHGINTSELQSVSSMQGPPGEIITRRDYFLTQGQAFLIYFK
                     LLFLPLRQNLDYDIAVSKSIFSPPETFAGFMLMSGIVFLCILFFKKHRFLSFSLAFFI
                     ISILPQSSIVPKPDLVVEHRLYLPLIGFAFFLPSFLYYISRKKEYVTIFLTVLLCFYG
                     VLTYQRNNVWKDPLTLWNDTVSKSPNKARPYLNRGLAYAAKGDIKNALSDYTMAIKIN
                     PWYIEAYNNRGILFASTKNYQGALSDFNKAIELKPDYALTYNNRGVLYSSLHEWNAAF
                     TDFDMALKLKPVYVDALKNRGIAFLVNKKIDMAIQDFSSAIEIYPENGQLYFHRAVAL
                     LTRGDKKSATGDLKHAMKLGYPIPQELAQILNEQ"
     gene            complement(1269..>2214)
                     /locus_tag="PLW07_06770"
     CDS             complement(1269..>2214)
                     /locus_tag="PLW07_06770"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOL50268.1"
                     /translation="LASQTPNRFFSSVLSDIASNIREGTSFSQALSKHPRVFSPLYTA
                     LVVSGEESGNLVEVLKDLSSELEDQLTLLRKVRQAVSYPAVILLFFVAVVAFVFLYLV
                     PKFQEIFESFGAQLPLFTRIILQISRFSLKFFPFLLVGLILLGVFLSWYSRTKSGSRR
                     IDGLKLRLPVFGDLFLKVSLARFSRSLATLLQGGVSIVAALDIVARTAGNTILEDYIE
                     RIRQGVIKGELLGDEMKKYRLFPAMMVRMVRVGEETGRTDDMLTRVSRFFRDEVDVTL
                     NILSSILEPVLIIGLGVIVGTVVLAIYLPIFKLAATVR"
BASE COUNT          687 a          475 c          380 g          672 t
ORIGIN      
        1 tattatgtgt tctgtttctg ccattgcggg catgttcaca aaagaaatag cgatattttt
       61 cccatttctc atattgatgt actttttctg gttcagaaaa aaattgaact gtgttataaa
      121 aacatgggaa gttattttct attcagtttt tttgttttca atcattccgg caatactttt
      181 actcactcat ggaataaata cttctgaact tcaatcggtg agttcaatgc aggggccacc
      241 cggtgaaatt attacacgca gagattattt tctcacccag ggacaggcat ttttgattta
      301 tttcaagctt ttgtttctac ccctgagaca aaatcttgat tacgacatcg ctgtttcaaa
      361 atccattttt tctccgcctg aaacatttgc aggattcatg ttaatgtcag gcattgtttt
      421 cttgtgcatt ttatttttta aaaaacacag atttctttct ttttcacttg cctttttcat
      481 catatctatt cttccgcaat caagcattgt tccaaaacca gatcttgttg tggaacacag
      541 attgtatctt cctttaatag gttttgcatt tttcctgcca tcttttctgt attatatttc
      601 aagaaaaaag gaatatgtga caatattttt gacagtactc ctgtgtttct atggtgtttt
      661 gacgtatcaa aggaataatg tatggaagga cccattgaca ctctggaatg atacagtttc
      721 aaaatctcca aataaagcaa gaccatactt aaatcgcggt cttgcttatg cagcaaaggg
      781 cgacatcaag aacgccctca gtgattacac aatggcaata aaaatcaatc cctggtatat
      841 tgaagcatat aacaatagag gaattctttt tgcatcaaca aaaaattatc agggtgccct
      901 ttctgatttt aataaagcca ttgaattgaa acctgattat gcacttacct ataacaatcg
      961 aggagtacta tattcatctc ttcatgaatg gaacgccgct tttacggatt ttgacatggc
     1021 attgaaactg aaacctgtat atgttgacgc tctgaaaaat cgtggtatcg ctttcctggt
     1081 taacaaaaaa atcgatatgg caattcagga cttctcttct gcgattgaga tttatcctga
     1141 aaatggacaa ttatattttc acagagcagt tgcattactc accagaggag ataaaaaatc
     1201 tgcaactgga gacctcaaac atgcaatgaa actcgggtat ccaataccgc aggaactggc
     1261 acagattctc aacgaacagt agccgcaagt ttaaaaattg gaaggtaaat tgcaagtaca
     1321 accgtaccaa caatcacacc cagcccgata atcagcacgg gttcaagtat tgatgaaagt
     1381 atgtttaagg tcacatccac ttcgtccctg aaaaacctcg aaaccctggt cagcatgtcg
     1441 tcggttcttc ctgtttcttc acccaccctc accattctta ccatcattgc tggaaacaat
     1501 ctatattttt tcatctcgtc gccgagtaat tctcccttta taacaccctg tctgattctt
     1561 tcgatgtaat cttccagtat cgtattacca gctgtcctgg caacgatatc aagggctgca
     1621 acaatactga ctcctccctg aagaagagtc gcaagtgaac gggaaaatct tgctaaacta
     1681 actttcagga aaagatctcc aaaaactggt agccgcaatt tcaacccatc aattcttctg
     1741 cttccagatt ttgttctgct ataccatgaa agaaaaactc ccagtaaaat cagcccaacc
     1801 aacaaaaaag gaaaaaactt cagcgaaaac ctgcttatct gcaggataat tcttgtaaat
     1861 aacggcagtt gcgccccaaa actttcgaaa atttcctgga attttggtac gagatataaa
     1921 aaaacaaatg caactacagc cacaaaaaat agcagtatta cagcagggta actgacagcc
     1981 tgacgaacct ttcttaaaag agtcaactgg tcttcgagtt ctgatgaaag gtcttttaaa
     2041 acctccacaa ggttaccgct ttcttcacct gacaccacaa gggccgtata caacgggctg
     2101 aaaacccttg gatgctttga cagagcctga gagaaactgg ttccttctct gatattactg
     2161 gctatatctg aaagcacaga agaaaaaaac ctgttcggtg tctgagaagc aagg
//
LOCUS       DAORYU010000243         2214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_33016_length_2214_cov_2.989810, whole genome shotgun sequence.
ACCESSION   DAORYU010000243 DAORYU010000000
VERSION     DAORYU010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33016_length_2214_cov_2.989810"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="PLW07_06775"
     CDS             complement(<1..453)
                     /locus_tag="PLW07_06775"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOL50269.1"
                     /translation="MFFFKITNKDGFARAGVFLSPHGEVQTPCFMPVATTASVKLLTS
                     EEVKSLGFDAIILNAYHLYLRPGLEIIENSGGIHEFMNWHGLAITDSGGYQMFSLPGS
                     KIDENGIILKAPESGAKYYFTPEKMVEIEQKIGADIIMCLDYCPKIWDN"
     gene            complement(485..1483)
                     /gene="queA"
                     /locus_tag="PLW07_06780"
     CDS             complement(485..1483)
                     /gene="queA"
                     /locus_tag="PLW07_06780"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOL50270.1"
                     /translation="MSKIFHYDLPSDRIAQRPLEKRDEARLMLVDRQRNSIEHFVFKD
                     IVNFFSSGEVIVLNDSRVIPSRLYGKKDTGGHVELLLLRKVDEKNWEVLIRGKIKSGT
                     KLLFGEISAVVLGRNKIGSWLVQFDADDQKISDIGRMPIPPYMKRMSDERDSIDYQTV
                     YARKHGSIAAPTAGLHFTHQIFEQLRQKGVITCFLTLHIGWASFRKIEFDGEKPVVLP
                     EFFEISEETASVINKAIHSGKKICAVGTSTVRAIESSFANGAVVARKGSTDLFIEPGY
                     HFSCINRMITNFHLPGSTHLYMVCAFGGTSLIQRAYLTAIEKEYRFYSYGDTMLII"
     gene            complement(1480..1905)
                     /locus_tag="PLW07_06785"
     CDS             complement(1480..1905)
                     /locus_tag="PLW07_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50271.1"
                     /translation="MAKSFAYSLLMNKKDVRFISAEYIDSEIPSGAKIMYTKDPWIFE
                     VPPVNPLKHSVSIIPEKDLEKAETGSFLILGELQYFLTSGNRVREMEKKIWQMKEKGF
                     IIRKIFASFPRVGPFKFDYRWTLHDMIYAHPIILLFYKP"
BASE COUNT          702 a          479 c          345 g          688 t
ORIGIN      
        1 attatcccag atttttgggc aataatcaag acacatgata atatccgcac ctatcttttg
       61 ttcgatttcc accatttttt ctggcgtaaa ataatatttt gcaccacttt caggtgcttt
      121 caatattatt ccattttcat ctatttttga accaggcaaa ctgaacatct ggtaaccgcc
      181 actatctgta atggcaagtc catgccagtt cataaactca tgaatccctc cagaattttc
      241 aataatttct aatccgggtc tgagatacaa atggtaggcg ttcaaaataa tagcatcaaa
      301 accgagtgat ttcacctctt ctgaagttaa aagtttcaca cttgccgttg ttgcgactgg
      361 cataaaacag ggcgtttgaa cttctccatg aggagacaaa aatacaccgg ctctcgcaaa
      421 cccatcttta ttggtgattt tgaagaaaaa catcgtggtg taattttaac acaaatacta
      481 ttttttaaat aatcaacatt gtatcaccat atgaataaaa tctgtattct ttttcaattg
      541 ccgttaagta tgctctttga ataagtgacg tccctccaaa ggcacatacc atatacagat
      601 gagtagaacc tggaagatga aagtttgtta tcattctatt tatgcaactg aaatgatatc
      661 ctggttcaat aaacaaatct gttgaaccct tgcgggcaac aactgcgccg ttggcaaaag
      721 aactctctat ggctctcaca gtacttgttc caactgcgca aatctttttc ccggaatgta
      781 tcgctttgtt aatcacgctg gctgtttctt ctgaaatctc aaaaaattca ggtaatacca
      841 ctggcttttc tccatcaaat tctatcttac gaaaagatgc ccaacctatg tgaagagtta
      901 aaaaacatgt aataacgccc ttttgtctca attgttcaaa aatctgatgg gtaaaatgca
      961 atcctgctgt tggtgctgca atagacccat gctttctggc gtatacagtt tgataatcaa
     1021 tactgtctcg ttcgtcagac attcttttca tatatggcgg aattggcatt cttccaatgt
     1081 cagaaatttt ctgatcatct gcatcaaatt gaaccagcca ggatccaatt ttgtttcttc
     1141 ccaataccac tgcggaaatc tccccaaata gtaatttcgt tccagattta atttttccac
     1201 gaataagcac ttcccagttt ttttcatcta cttttctcaa cagaagtaat tcgacatgac
     1261 cgcctgtgtc ttttttacca tacaaccttg aaggaatgac cctgctatcg ttcaaaacaa
     1321 ttacctctcc ggacgagaaa aaattcacaa tatctttgaa tacaaaatgt tctatagagt
     1381 ttctctggcg atccacaagc attaaacgag cctcatccct tttctctaaa ggtctttgtg
     1441 caattctgtc agacggcagg tcataatgaa atatcttgct catggtttat aaaacaagag
     1501 tattattgga tgggcataaa tcatatcatg aagtgtccac cggtaatcaa atttaaacgg
     1561 tccaacccgg ggaaaactcg caaaaatttt tctgataata aacccttttt ctttcatctg
     1621 ccatattttt ttctccattt cgcgcactct attgccagaa gtcaaaaagt attgaagttc
     1681 accaaggatt aaaaaacttc ctgtttccgc tttttccaga tctttttcag gaataattga
     1741 cacagaatgt tttaatggat taacaggagg aacttcaaaa atccatggat cttttgtgta
     1801 cattatcttc gccccggatg gaatctcact gtcaatatat tctgcagata taaaacgcac
     1861 atcttttttg ttcatcaaca atgaatatgc aaaagatttt gccatacttc ctaaaataaa
     1921 aacgccacaa acacttacta caaagaattt ttttggaaaa cttttgcatt gtaacatctc
     1981 atcaaacaag gcgccggcac tcaagctcaa aaatggcagg acaagcaaat aataacgcgc
     2041 atatttaaac ctccagcaga cattaacggt attatcatca acaagaaacc tgtaagaact
     2101 attctatcag aggcatttct cctctttgcc tgtaagaaga taccacagat ggctaatata
     2161 aaaatgagcc agataccggt ccacagaccg ctgaaaaaac cagagaaaga atag
//
LOCUS       DAORYU010000244         2206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_33173_length_2206_cov_3.676430, whole genome shotgun sequence.
ACCESSION   DAORYU010000244 DAORYU010000000
VERSION     DAORYU010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33173_length_2206_cov_3.676430"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="PLW07_06790"
     CDS             complement(<1..619)
                     /locus_tag="PLW07_06790"
                     /inference="COORDINATES: protein motif:HMM:NF012783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOL50272.1"
                     /translation="MNTEGAKSLNRNVYVLTIDGTINPVVYYKVKRALANIKKQNGQC
                     LVIMMDTPGGLLISTRKIVQEILNSEIPVVCYVAPRGAQCASAGTFIALSCHIVAMAP
                     ATNIGAAHPVSIGKEMDKTMEEKVINDTVSFIKSICHHTGRNEKWAEKAVRESISTTA
                     EEAVKINVADFIATDVEDLINKLDQKQVKLASGRTVILFVSSITVL"
     gene            complement(652..1740)
                     /locus_tag="PLW07_06795"
     CDS             complement(652..1740)
                     /locus_tag="PLW07_06795"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOL50273.1"
                     /translation="MNNEINIKWTRIRDFLSEKNFSGIILNRISNFAWFTGGRKNYVA
                     FNTEYGASSLLVTGKKIFLLSNNIEATRLKQEELVDIPIEDIVISWHQDGTLFENARK
                     IVGGNIAIDKPGEKFEYAAIDHLHFPLTNEEIERFHNLGQKVTQIVREVCHEIKPGMT
                     ENEVAGNLSNAFWACGTIPVVLLIAADERIARFRHPVATDKKIEKYVMVVVCVYGNGL
                     IVSMTRLVSFSKLPDEILKKHEAVCRVDATFINSTVPGKTIGEVFKAGIDAYRITGFS
                     EEWEKHHQGGPCGYKTRYYRATAESSDIVKPGYAFAWNPSITGTKSEDTIIVATDKNL
                     IITEDNKWPKIEINTGGTCISRPDILVR"
     gene            1944..>2206
                     /locus_tag="PLW07_06800"
     CDS             1944..>2206
                     /locus_tag="PLW07_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOL50274.1"
                     /translation="MRNEGMDIHVSYSLDVLLDKKLLEKVDLIVPVWTAGNITADQEK
                     GLLLSLEFGVVIGGWYVGMGDAFRRNPSSQFMVGGQWLSHFGS"
BASE COUNT          599 a          439 c          389 g          779 t
ORIGIN      
        1 ggagaactgt aatacttgac acaaaaagta ttacagttct ccctgaagca agttttacct
       61 gtttttgatc aagcttattg ataagatctt caacgtcagt tgctataaaa tcagcaacat
      121 taatttttac tgcttcttca gccgttgttg aaatgctttc cctgactgct ttttctgccc
      181 atttttcatt tctgccagta tgatgacaga tactcttgat aaaacttaca gtatcgttga
      241 taactttttc ctccatcgtt ttatccatct cttttcctat gctcaccggg tgggctgcgc
      301 caatatttgt tgcaggagcc atggcaacaa tgtgacagga aagcgcgata aatgttcctg
      361 ctgaagcaca ttgtgctccc cttggtgcga cataacatac aacagggatt tctgaattta
      421 aaatttcctg gactattttc cttgttgaaa taagaaggcc gccgggggta tccatcataa
      481 taaccagaca ctggccattt tgttttttga tatttgcaag ggctcgtttt accttataat
      541 aaacaacagg attaatagtt ccatcaattg ttaaaacata aacatttctg tttagtgatt
      601 ttgcaccttc tgtattcact gaaaaaaatc ccggaatcag taaacagcaa atcaacgcac
      661 aagtatatcg ggtcttgaaa tacatgttcc tcctgtattt atctcgattt ttggccattt
      721 attatcttcc gtaataataa ggttcttatc tgttgctact atgattgtat cctctgattt
      781 ggttccagta atagaaggat tccaggcgaa agcgtaccct ggtttaacaa tatcagaact
      841 ttctgctgtt gctcggtaat atcttgtttt ataaccacat ggtccaccct gatggtgttt
      901 ttcccattct tcagaaaaac ctgttattct gtatgcgtca atgcctgcct taaatacttc
      961 tccaattgtt tttcccggta ctgttgagtt aataaatgtt gcatcaacac gacacacggc
     1021 ttcatgcttt tttaagattt catcaggcag ttttgaaaaa ctgacaagtc ttgtcataga
     1081 tacaatcaat ccatttccat agacacacac gacaaccatt acatattttt cgattttttt
     1141 gtcagttgcc acgggatgtc tgaatcttgc aattctttcg tctgctgcga taagaagtac
     1201 tacaggaatc gttccgcatg cccagaatgc gttactcaaa tttccagcaa cttcattttc
     1261 tgtcatacct ggttttattt cgtgacacac ttctcgcaca atttgtgtaa ccttttgtcc
     1321 gaggttatgg aatctttcaa tttcttcatt tgttaatgga aaatgcaggt ggtcaatggc
     1381 agcatattca aatttttctc caggtttatc aattgcaatg ttgccaccta caatttttcg
     1441 cgcattttca aataatgttc catcctgatg ccagcttatg acaatgtcct ctatcgggat
     1501 atcaaccagt tcctcctgtt ttaatcttgt tgcttcaatg ttatttgaga gtaaaaatat
     1561 tttctttcct gtcacaagca ggctgctggc gccatattct gtattaaatg ccacataatt
     1621 tttcctgcca ccggtgaacc atgcaaaatt agaaattcta ttaaggataa tgccagaaaa
     1681 atttttctct gagagaaaat cccttattct tgtccatttg atattgatct cattattcat
     1741 gatatcctcc tcattgatac catattatat caaagaacat tttgaataag aaataataag
     1801 tatatttggc aattatttat ttgtttgcta aaatcttacc ataaatttta ccggaggcgg
     1861 gtatcaagaa atttatcctt tttctgtgtg gtggctggga aggtcacgag ccgcaagagt
     1921 gtgttcatct tttttcagaa attatgagaa acgagggaat ggatattcat gtctcatatt
     1981 cccttgatgt tttacttgat aaaaaattac ttgaaaaagt ggaccttatt gttcctgtct
     2041 ggacagcagg gaatatcaca gccgaccagg aaaaaggatt gcttctttca ttggaattcg
     2101 gtgttgtaat tggtggctgg tacgtaggaa tgggtgatgc atttcgtcgc aatccttctt
     2161 ctcaatttat ggtgggtggt caatggcttt ctcattttgg cagtat
//
LOCUS       DAORYU010000245         2197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_33365_length_2197_cov_3.492997, whole genome shotgun sequence.
ACCESSION   DAORYU010000245 DAORYU010000000
VERSION     DAORYU010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33365_length_2197_cov_3.492997"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="PLW07_06805"
     CDS             complement(<1..409)
                     /locus_tag="PLW07_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50275.1"
                     /translation="MFPNKYRFCDVASVFIIIFSTFLIYSNTFSVPFQFDDLACITGN
                     WRILHLEKIGELFRYWSTRFITYLTFAIDYKIHHFHVTGYHIINLLIHISCGLLIWHI
                     VKKTLQGMFENNTTSVVALFSSLIFVMHPVQTQA"
     gene            490..906
                     /locus_tag="PLW07_06810"
     CDS             490..906
                     /locus_tag="PLW07_06810"
                     /inference="COORDINATES: protein motif:HMM:NF028042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOL50276.1"
                     /translation="MKKRKGVIEVKKTRNSKKGFTLIELMVVVIIVGILAAVAIPIYR
                     SNIKKAAASEGAALLGAVLTAQKVYYAENSTYTSNKSDLNVETTGNKYFSDYTLSGVS
                     ATGFTASTTGSGTASGVTVTMTYTNAGGATISYSGL"
     gene            926..1930
                     /gene="pilM"
                     /locus_tag="PLW07_06815"
     CDS             926..1930
                     /gene="pilM"
                     /locus_tag="PLW07_06815"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HOL50277.1"
                     /translation="MNVCLGLDIGSHYIKIVEGYAKKGKFFVKTAGIIHNPLPRGHIN
                     LNENSQKQFSVFLKEFLRKISIKRKETVCGISGNGLIIHYFDIPDIPSEEIESTVELE
                     VLQVIPGGVEKIEYDYTVLPSVQGKRTVMLVGLPKPKCDFFVNTLLLSGLRPVIMDIG
                     GLALANCYRTLSNNSYGDVAIIDVGASHTVLAITEKNGFVFVRDIDFGGDNVNIEISK
                     LKKISVSEAEQFKKNPGFRDEVEKILRNTMGDILQEILTSIKYVETRTQKKIEKFLLT
                     GGSSLLPGFVKIVEENLGISGDLWKPMEGLYQYCKTDQPQCLEVCFSQALGLVARKLI
                     "
     gene            1927..>2197
                     /locus_tag="PLW07_06820"
     CDS             1927..>2197
                     /locus_tag="PLW07_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50278.1"
                     /translation="MIEINLLKKRQQQYYQRVFLIRVIFIYIIGLVCILILAGVSFLS
                     NRIAIRSTLASIQHYNQKIRDEYGAVQELEKYKNQMDEIAKRLFLA"
BASE COUNT          715 a          317 c          491 g          674 t
ORIGIN      
        1 ctgcctgtgt ttgcacagga tgcataacaa atatgaggga tgaaaaaagg gctacaactg
       61 atgtggtatt attttcaaac ataccttgga gagttttttt tacaatgtgc catatcagaa
      121 ggccacaact gatatgaatg agtaaattaa taatgtgata acctgtaaca tgaaaatgat
      181 gaattttgta gtcgattgca aaggttaaat aagtgatgaa tcttgttgac cagtatctga
      241 acaattcacc aattttttcg agatgtaaaa ttctccagtt ccctgtaata catgcgagat
      301 catcaaactg aaatgggaca gaaaatgtat ttgaataaat aaggaacgta gaaaaaataa
      361 tgataaacac cgacgcgaca tcgcaaaatc tgtatttatt gggaaacatt ttgattgtta
      421 gatagtttag cacagaattc gaattttgat aatctatttg acagcgaagg tttcagtgta
      481 gtaaaatcta tgaaaaaaag aaagggggtg attgaggtga agaaaacaag aaattcaaaa
      541 aagggcttta ctcttattga gttgatggtg gtggtaatta ttgttggtat tctggctgct
      601 gtagcaatac ctatttacag aagcaacatt aagaaagcag cagcatcaga aggtgcggca
      661 ttgcttggtg cagtgcttac tgctcagaag gtttattatg cagaaaatag cacttatacc
      721 agtaataagt cggatcttaa tgttgaaacc actggtaata aatatttctc agattacact
      781 ttgagcggag tcagtgccac agggtttact gcatcaacaa cagggtccgg gactgcctct
      841 ggtgttacag tgacaatgac ctataccaat gctggtggtg cgacaataag ctattccggt
      901 ttataatggg ggtttgccgg gttttatgaa tgtttgcctt ggattggata ttgggtctca
      961 ttatattaag attgttgagg gttatgcgaa aaaagggaag ttttttgtaa aaacagcggg
     1021 cataattcat aaccctttgc cacgtgggca tattaatctt aatgaaaata gccagaagca
     1081 attttctgtt tttttgaaag aatttttaag aaaaattagt atcaaaagaa aagaaaccgt
     1141 ttgtggaatc agtggaaatg gtttgataat tcattatttt gacattcctg atattccttc
     1201 cgaagaaatc gaaagtactg ttgagcttga agtgcttcaa gtaattcctg gcggagttga
     1261 aaagattgaa tacgattaca ctgttttacc ttctgttcaa ggcaaacgga ctgttatgct
     1321 tgtgggactt cccaagccaa aatgcgactt ttttgtcaat acacttttgt tgagcggttt
     1381 gaggcctgtc ataatggata tcggtgggct tgcgcttgca aattgttaca ggactttgag
     1441 taataattct tatggggatg ttgctatcat tgatgtgggt gcgagtcata cagttcttgc
     1501 aattactgag aaaaacggat ttgtttttgt cagggatatt gattttggag gagataatgt
     1561 taacatcgaa atcagcaaat taaagaaaat ttctgtgtca gaagcagagc aattcaaaaa
     1621 aaaccctggt tttcgggatg aggttgagaa aattctacgg aatactatgg gtgatatttt
     1681 gcaggaaata ttaacatcca tcaaatacgt tgaaacacga acacagaaaa agatagaaaa
     1741 gtttctgctg acaggtggat caagtctgct tcctggattt gtaaaaattg tagaagaaaa
     1801 tctcgggatt tcaggggatc tctggaaacc aatggaggga ttgtaccagt actgcaaaac
     1861 agatcaacct caatgtctgg aagtatgttt cagtcaggcg ctgggacttg tggcaaggaa
     1921 acttatatga tagagatcaa ccttttgaaa aagcggcagc aacaatatta tcagagggta
     1981 tttttgataa gggtgatttt tatttatatt attggtcttg tgtgtatttt aattcttgct
     2041 ggtgtttcat ttctttcaaa cagaattgcg atcaggtcaa ctctggcaag tatccagcat
     2101 tacaatcaaa aaatcagaga tgagtatggt gctgtccagg aacttgaaaa atataaaaat
     2161 cagatggatg aaatcgcaaa aagattgttt cttgctc
//
LOCUS       DAORYU010000246         2189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_33528_length_2189_cov_3.694002, whole genome shotgun sequence.
ACCESSION   DAORYU010000246 DAORYU010000000
VERSION     DAORYU010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33528_length_2189_cov_3.694002"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="PLW07_06825"
     CDS             complement(<1..521)
                     /locus_tag="PLW07_06825"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOL50279.1"
                     /translation="MDKPNLIFVVFDTLRADYLGCYGNKRIYTPNFDAFAGESVIFDN
                     VYPESLPTIPVRRGLHTGRRCYPFRDYQILKWCPIKLPGWQPVSNNEDTIAENLVNNG
                     YYTGFITDTSAYFTPGMNFTRGFLQWEFIRGVEGSIYHSPSRVTSEILKKYGGDINKL
                     NKPYPKYFTLNAAA"
     gene            complement(553..1929)
                     /locus_tag="PLW07_06830"
     CDS             complement(553..1929)
                     /locus_tag="PLW07_06830"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HOL50280.1"
                     /translation="MKKPNIIFILADDMGYGDFSFFNQGLSDTPVLESIYQEGVCLTQ
                     HYSASPVCSPARAALLTGRYPHRTGCIDTLQMNGLDRLAPDEITIADILKANGYATGL
                     IGKWHLGAIEKKYHPNQRGFSEFVGFRGGWQDYYQWNLYYNEQLVRADGRYLTDVFTD
                     EALRFIKRHKNDPFFLHITYNAPHLPLQSPEEDVRFFLEKGLSEGVAYIYAMIRRMDR
                     GIGQILEELRKSGLENQTIIFFTSDNGPQFKGGGKCLMRYNHNFRGCKGNVYEGGIRV
                     PMLVRWPGVINRGEVIDQMVHFVDWFPTILDICGLKMPRGSKNIDGKSILSILQGNKP
                     VNSKRFWQWNRLYPVCNSNIAMRDGDWKLVKPPIPSTMKVPIELVEIDREYAYHPEQF
                     NDIIKMSFPAFDIGENTPQLFNLQTDPLEQVDLASRYPEKVKEMEQEMNEWFEEVESE
                     RKLRIQDK"
     gene            complement(1933..>2189)
                     /locus_tag="PLW07_06835"
     CDS             complement(1933..>2189)
                     /locus_tag="PLW07_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50281.1"
                     /translation="DSHGYSESWKEYRGKIDLACLWCVKTEEMIENLQPINVFLHHFQ
                     KFSPGQFSCNVDIQRLMGFLKEKFNQVNFYHPVEEKKVIL"
BASE COUNT          634 a          510 c          387 g          658 t
ORIGIN      
        1 gccgcggcat taagggtaaa atattttgga tatggcttgt tcaatttatt gatgtcccct
       61 ccatattttt taagaatctc agaagtcacc ctggacggtg aatgataaat acttccctcc
      121 accccgcgaa taaattccca ctgcaaaaat ccacgggtaa aattcattcc tggagtaaaa
      181 taagcagagg tatcagtaat aaatccagta tagtagccat tgttcaccag gttctctgca
      241 atggtgtctt cattgttgct gacgggttgc cagcccggta gtttaatggg acaccatttc
      301 aaaatctgat agtccctgaa aggataacat cgtctgccag tgtgaaggcc gcgacgaaca
      361 ggaatggttg gaagggattc aggatacaca ttatcgaata tcacggactc acctgcaaaa
      421 gcatcaaaat tcggcgtgta aattcttttg tttccatagc aaccaagata atctgcccgc
      481 agggtatcaa aaacaacaaa aattagatta ggtttatcca tctggccccc cggtaaaatc
      541 taccaaaaat tatcatttgt cctgaatacg caattttcgt tcagattcaa cttcttcaaa
      601 ccattcattc atttcctgct ccatttcttt caccttttca ggataccttg atgcaaggtc
      661 tacctgttcc aatgggtctg tctgcaaatt aaaaagctgg ggtgtatttt ctccgatgtc
      721 gaatgcagga aaactcatct ttatgatatc attaaattgt tctggatgat aggcatattc
      781 tcgatcaatt tccaccagtt caatcggcac tttcattgtt gaaggaattg gcggcttaac
      841 caatttccaa tcaccgtctc tcatggctat attggaatta cataccgggt acaatctgtt
      901 ccactgccag aatctcttgc tatttaccgg tttattaccc tgcaaaattg acaggattga
      961 ctttccatca atgttctttg aaccccgcgg catttttaat ccgcagatat caagaattgt
     1021 tggaaaccaa tcaacaaaat gaaccatctg gtcgattact tcacccctgt taatcacccc
     1081 tggccaacga accaacatcg gaactctgat tcctccctca taaacatttc ccttacaacc
     1141 cctgaaattg tggttatatc gcatcagaca ctttcccccg cctttaaact gtggtccatt
     1201 gtcgcttgta aaaaaaatta ttgtttgatt ttcaagtcct gatttccgca attcttccag
     1261 gatttggcca atacccctgt ccatccgacg gatcatagca taaatgtacg caaccccttc
     1321 actaagtccc ttttcaagaa aaaacctcac atcctcctct ggagattgaa gtggaagatg
     1381 tggagcattg taagtaatat gcaggaaaaa tgggtcattc ttatgtctct tgataaacct
     1441 gagcgcttca tctgtaaaaa catctgtcag ataacggcca tcagccctaa caagttgctc
     1501 attataataa agattccatt gataataatc ttgccatccc cctctgaagc ccacaaactc
     1561 actaaacccc cgctgatttg ggtgatattt tttctcaata gcaccaagat gccatttccc
     1621 gatcagtccc gtagcataac catttgcttt caatatgtcg gcaatagtaa tttcatccgg
     1681 cgcaagtcgg tcaagcccat tcatctgaag ggtatcaatg caacctgtac gatgaggata
     1741 tcttcctgtc agcagtgctg ctctggctgg actgcaaaca ggtgatgccg agtaatgctg
     1801 ggttaaacaa acgccttcct ggtagatgga ctcaagcaca ggcgtgtcag aaagaccctg
     1861 attgaaaaaa ctgaaatcac catatcccat gtcatctgcc agaataaaaa tgatgtttgg
     1921 ttttttcatg aattatagga tcactttttt ttcttctact ggatggtaaa aatttacctg
     1981 attaaatttt tccttaagaa atcccattaa acgctggata tcaacattgc aggaaaattg
     2041 tccgggagaa aatttttgaa aatgatgcaa aaatacatta atcggctgca aattttctat
     2101 catttcttct gttttcacgc accataaaca tgcgaggtct atttttcctc tgtactcttt
     2161 ccaggattct gaatatccat gagagtccc
//
LOCUS       DAORYU010000247         2175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_33854_length_2175_cov_3.725943, whole genome shotgun sequence.
ACCESSION   DAORYU010000247 DAORYU010000000
VERSION     DAORYU010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33854_length_2175_cov_3.725943"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(243..1460)
                     /locus_tag="PLW07_06840"
     CDS             complement(243..1460)
                     /locus_tag="PLW07_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50282.1"
                     /translation="MRIFKFLVLALIFAGVAFCQDANPQEKPVAAIVADWFKYSHPDV
                     LLTRILKTYSLDGKGQKSQLKLVSVFRDKPSSKDLSKAYAEAFGFKVCDSIEEALTLG
                     KGMLAVDGVIISTEWCDYPVSPTGQVMYPKRRLFEEVVKVFQKTNRVVPVFIDKHIAD
                     NWQDIIWIYNTAKKMKIPLMAGSSLPVSWRYPPVDVDTHSKLKEIIGISYHTLDGYGF
                     HGLEMIQCLAEQRKGGEIGVKSVQCLTGQCVWNAAGKLYDENLLNMAACKVRKIPEGK
                     TLKDMVKDPVLFVVNYNDGLRACLFTLNNFASQWAAAWKYADGKTDSSLFKLDDNLST
                     LMHFANQMHGIENMIITGKPAWPVERTVLTSGVLDALLISKLQSGKLINTPYLKISYR
                     PEWKWQQPEGVPE"
     gene            1526..>2175
                     /locus_tag="PLW07_06845"
     CDS             1526..>2175
                     /locus_tag="PLW07_06845"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOL50283.1"
                     /translation="MGKLDVTIVGGGMITNDLLLPAIYHLQRTGIVDSIQICALNSAP
                     LKYLRDNPELQQAFPGQSFTPYPDFSEPEQKNYPELYRDVISKMKPRQVVVVAMPDNL
                     HYEVVIEALKHNQHVLCVKPLVLKYTQAAEIEEIVKEKGLFVGVEYHKRFDRRSLMAR
                     RAYRLGQFGEFAMGDAKLIEPYYYRHSNFQNWFTVDRTDPFVYIGCHYVDLVYFIT"
BASE COUNT          688 a          463 c          392 g          632 t
ORIGIN      
        1 gttcacacac agaactgatt cgcatttcgt accaggactt tatcaatttg gtaaaaccaa
       61 acgtactaag ttttatcaaa taatggggaa agcacaatat aaacgttgga gaaattttaa
      121 aattgatgtt tgtgtgcaac ataagaattg ttcaacctga tatttttctc aaaacacatt
      181 ctccacaaca taaatataaa catttgtgtt tcttaaccaa cccttggtcg gaaataagat
      241 tcttattctg gaactccttc tggctgttgc cacttccatt caggtctgta tgaaattttc
      301 aggtatggcg tattgataag ttttcctgat tgtaactttg aaatcaaaag agcatcaaga
      361 acaccgcttg taagaaccgt tcgttccaca ggccaggcag gtttaccagt aatgatcatg
      421 ttctcaatcc catgcatctg attggcaaaa tgcatgaggg ttgagagatt gtcatcaagt
      481 ttaaacaaac ttgaatccgt ctttccatct gcatatttcc atgctgctgc ccactgagac
      541 gcaaagttgt tcaatgtgaa aagacaagcc cgcaatccat cattgtaatt gactacaaaa
      601 agcactgggt ctttgaccat atctttcagg gtcttacctt caggaatttt tctcacctta
      661 caggcagcca tgttcaaaag attttcgtca tacaatttgc ctgcagcatt ccagacacat
      721 tgacctgtta gacattgaac tgacttaacc cctatttctc caccctttcg ttgctctgca
      781 agacattgaa tcatctcaag accatgaaat ccgtacccat caagcgtatg atatgaaata
      841 ccgatgatct ctttcaattt tgaatgtgta tcaacatcaa ccggaggata tcgccacgaa
      901 acgggtaaag atgaacctgc catcaggggt attttcattt tttttgcagt attataaatc
      961 catataatat cctgccagtt atccgcaata tgcttatcaa taaaaacagg aacaaccctg
     1021 ttggtttttt gaaatacctt taccacctct tcaaacaacc ttcgtttcgg atacatcacc
     1081 tgacctgtag gcgacacagg ataatcgcac cattctgtcg aaataatcac tccgtcaact
     1141 gcaagcatac ctttcccaag tgtcaacgct tcttcaatac tatcacacac tttaaaccca
     1201 aatgcttctg cgtatgcttt acttaaatcc ttgctggaag gtttatccct gaacactgaa
     1261 acaagtttca attgcgactt ctgacccttt ccatcaagag aatatgtctt gagaattctt
     1321 gtaagcagta catcaggatg tgagtattta aaccagtcag cgacaattgc ggcaacaggt
     1381 ttttcctgcg gattcgcatc ctgacagaaa gcaacgcccg caaaaatcaa tgcaagaaca
     1441 agaaatttga aaattctcat attaatcgct aaaaaaaagt aatgttataa taaatactat
     1501 cataaaacca tgaaaggagg aacagatggg aaaacttgat gtaacgattg ttggtggtgg
     1561 aatgataacc aatgaccttc ttctgcctgc gatttatcat cttcaaagga caggaatcgt
     1621 tgacagcatt cagatttgtg cattaaacag tgctccgttg aaatacctga gggacaatcc
     1681 tgaattgcaa caggcgtttc caggacaatc atttacacca tatcctgatt tttcagaacc
     1741 agagcagaaa aattatccag aattgtacag ggatgttatt tcaaaaatga agccaagaca
     1801 ggtcgtggtt gtcgcaatgc cagacaatct tcactatgaa gtagtaattg aagcactgaa
     1861 acacaatcaa catgtactct gcgtaaaacc ccttgttttg aagtacacgc aggccgctga
     1921 aatagaagaa atcgtaaagg aaaaaggtct ttttgtaggc gtggaatatc acaaacgatt
     1981 tgacagaagg tcgctgatgg caagaagagc gtatcgactt ggacagtttg gcgagtttgc
     2041 aatgggcgat gcaaaactta ttgaaccgta ttactacaga cattcaaatt tccagaactg
     2101 gttcactgtt gatagaaccg acccatttgt atacattggt tgtcactatg tcgaccttgt
     2161 ttactttatc actga
//
LOCUS       DAORYU010000248         2163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_34119_length_2163_cov_5.377135, whole genome shotgun sequence.
ACCESSION   DAORYU010000248 DAORYU010000000
VERSION     DAORYU010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34119_length_2163_cov_5.377135"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1424
                     /locus_tag="PLW07_06850"
     CDS             <1..1424
                     /locus_tag="PLW07_06850"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HOL50284.1"
                     /translation="QAIPIFRSRDRVVCGMVDPLDPETTEQIKKISGLEVETRLVKES
                     ELRKSFDKYFSQVGLDVISPVNQGSEKFYEPVKLRELDREATAESSVISMIEQAVELK
                     ATDIHLEVEEDGVRLRYRINGLLYEFPPPPLELYPAIVSHIKVLSNLDISEKRVPQDG
                     YFKARISGRSVDLRVSTFPTIFGEMVALRVLDKTNIITGLEQLGFLPETLHRWRMLLD
                     EPYGMLLVTGPTGSGKTTTLYSSLNEIDNVHKKVITVEDPVEYHLKNIDQIQINPKAG
                     LTFAVALRSILRQDPNVVMIGEIRDLDSASIAFRAAQTGQLVLATLHTNTAPGAVIRL
                     LDMGVESYLLASSLIGVLSQRLVRTICPNCKEVYKPLDEEIRELSPALVGRDIHFYRG
                     KGCHLCKGTGYGGRTGLHELMVMNEELRRTTMRDPSTSVLTEIARKFGMKTLREDGIQ
                     KVLQGITTISEVLYATKKDTDER"
     gene            1393..1827
                     /gene="nikR"
                     /locus_tag="PLW07_06855"
     CDS             1393..1827
                     /gene="nikR"
                     /locus_tag="PLW07_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HOL50285.1"
                     /translation="MQQKKTQTKDKIVRFGVSLEKDLLEIFDRKIKQKKYATRSKAIA
                     DLMRKEIIQQRWRTGKEVAGAIVFIYNHHRRNVVDKIIDIQHDFSNIVVSTQHVHLSH
                     DECLEIVVIKGKPQMVETLADNIRSTKGVEHCVVAFTSTGNL"
     gene            complement(1829..>2163)
                     /locus_tag="PLW07_06860"
     CDS             complement(1829..>2163)
                     /locus_tag="PLW07_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017951059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOL50286.1"
                     /translation="IGIMNIMLAIVTERTREIGIRRAVGAKKRDIINQFLTESVILSA
                     SGGFIGILLGIIIPKAVTKFAGMMTLITPWSILLSFSISVAVGIGFGLYPARKAANLD
                     PIQALRYE"
BASE COUNT          703 a          419 c          476 g          565 t
ORIGIN      
        1 atcaggcaat tcccattttc aggtccagag acagagtcgt ttgtggaatg gttgacccgc
       61 ttgatccaga aacaacagaa cagatcaaga aaatttctgg acttgaggta gaaacaagat
      121 tggtcaagga atcagaattg aggaaaagtt ttgataaata tttttcacag gttggccttg
      181 atgtcatcag tcctgttaat caaggttctg aaaaatttta tgaacctgtg aaactgaggg
      241 aacttgatag agaagcaact gctgaaagtt cagtgatttc aatgatagaa caggcagtgg
      301 aactgaaggc aactgacata catctggaag tagaggaaga tggcgtgcga ctgagatata
      361 gaatcaatgg acttctatac gaattcccgc ctccgccgct tgaactgtat cctgccattg
      421 tgtcgcatat caaggttctt tcaaatcttg atatttctga aaaaagggtg ccacaggatg
      481 gttatttcaa agcgagaatc agtggaagaa gtgtggactt aagagtttca acattcccga
      541 caattttcgg agaaatggtt gcactcagag tacttgacaa aacaaatatc attacaggac
      601 ttgaacaact tggcttttta cctgaaactc tacacaggtg gcgtatgctt cttgatgagc
      661 cgtacgggat gcttcttgtc acaggtccaa caggttcagg aaagacaaca accctttatt
      721 cctctcttaa tgaaatcgat aatgtgcaca aaaaggtgat tactgttgag gaccctgttg
      781 aatatcactt gaaaaacata gaccagattc aaataaaccc gaaggcgggt cttacgtttg
      841 ctgttgcgct tcgctcaatt ttaaggcagg acccaaatgt tgtgatgatt ggggagatca
      901 gagaccttga cagtgcttca attgctttca gagcagcaca aacaggtcaa cttgtgcttg
      961 caaccctgca cacaaatact gctccaggag cagttatcag attgcttgac atgggcgttg
     1021 aatcttacct gcttgcgtca tccttaatag gggtactgag ccagcgtctg gtaagaacaa
     1081 tatgcccgaa ttgcaaggaa gtgtataaac cacttgatga agagataagg gaattgagtc
     1141 cagccctggt gggtagagat atacattttt acaggggcaa gggatgtcat ctatgcaagg
     1201 gaactggcta tggcggtaga accggacttc acgagctgat ggtcatgaac gaagaactca
     1261 gaagaacgac aatgagagac ccttcaacat cagtacttac agaaattgca agaaagtttg
     1321 gcatgaaaac attaagagag gatggaattc aaaaagttct tcagggaatt accacaatat
     1381 cagaggtcct gtatgcaaca aaaaaagaca cagacgaaag ataaaatcgt aagatttggt
     1441 gtttctctcg aaaaagacct gttagaaatt tttgacagaa aaataaaaca gaaaaagtat
     1501 gccaccaggt caaaggcaat cgctgacctg atgagaaaag aaatcatcca gcagagatgg
     1561 cgaacaggaa aagaggttgc tggagccatt gtcttcatct acaatcatca cagaagaaat
     1621 gttgttgata aaattattga catacagcat gacttttcaa atatcgtggt atcaacccag
     1681 cacgttcatc tttctcacga cgaatgcctt gaaattgtgg tgataaaagg aaaacctcaa
     1741 atggttgaaa cccttgcaga caatatccgt tcaacaaagg gtgttgaaca ttgcgtggtt
     1801 gcctttactt caactggcaa cctgtaaatc attcgtatct cagggcttga attgggtcaa
     1861 gatttgcggc ttttcttgca ggataaagtc caaaacctat tccaacagcg acagaaatag
     1921 aaaaagaaag caaaatcgac catggtgtaa tcagtgtcat cataccagca aatttcgtga
     1981 ctgccttcgg tataataatt ccaagcagaa ttccgataaa acctccagag gcagaaagta
     2041 tcacggattc tgtcagaaac tgattgataa tatccctttt ctttgctccc actgcccttc
     2101 tgatgccgat ttctcttgtt ctttctgtaa caattgccag catgatattc ataatgccaa
     2161 ttt
//
LOCUS       DAORYU010000249         2150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_34380_length_2150_cov_6.707876, whole genome shotgun sequence.
ACCESSION   DAORYU010000249 DAORYU010000000
VERSION     DAORYU010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34380_length_2150_cov_6.707876"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /gene="lsrF"
                     /locus_tag="PLW07_06865"
     CDS             <1..621
                     /gene="lsrF"
                     /locus_tag="PLW07_06865"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011713263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HOL50287.1"
                     /translation="ILRSIIPPTYTNGIVIRASGGPSILKELSNEQIAMDIEDAIRLN
                     AAAVAVQVFIGGEYETQSVHNMTKLVDMGQRYGIGVLGVTAVGKELVRDAKYMRLACR
                     ICAELGATYVKTYYVEPDFETVTASCPVPIVIAGGKKIPEFDALKMAYNAIQQGACGV
                     DMGRNIFQAEDPVAMIQAVRMVVHKNAKPEEAYQYYKEKISQKQKG"
     gene            628..1371
                     /locus_tag="PLW07_06870"
     CDS             628..1371
                     /locus_tag="PLW07_06870"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOL50288.1"
                     /translation="MTTSFHHYVRSLTPMLSAGILAADVMHIGEEIEKLDGTGVKMLH
                     FDVMDGVFCPMITTGPCFIKGVKTKYLKDVHLMVQNPITKISQFAEAGADMITVHLES
                     CPDHIHALIQAIKKLKNVNSVEREIIAGVAINPGTPVDSIKNVLDVVDMITILAVNPG
                     WQGQSFIKTTAKKLQQAISLINESERDILLCIDGGVTKDNIHDIAQMAPDIVVAGSAI
                     FDGKDPQANARFLVETLKKGKAKEIIHET"
     gene            1361..>2150
                     /locus_tag="PLW07_06875"
     CDS             1361..>2150
                     /locus_tag="PLW07_06875"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOL50289.1"
                     /translation="MKPEPECTYDLKDRVAMVTGAAGAIGAGICRGLLKSNCRVAATD
                     LPGEKLDNFVKEYQEVFKEKIIGIPMDVTDPESVSTGIKKIVEIFGCLDIAVINAGIA
                     HVSSLKDMDIEEFQKLEKVNVEGTLLILKELARYFENQKTGGDIILISTKNVFAPGAS
                     FGAYSATKAAAHQLARIASIEFAPLGVRVNMIAPDAVFSDGARRSGLWEKIGPDRMKA
                     RGLDEKGLEEYYRNRNLLKAKITADHVANAVLFFASHQTPTTGAT"
BASE COUNT          715 a          390 c          508 g          537 t
ORIGIN      
        1 atactgcgct caatcatacc tcctacatat accaatggta ttgtgataag ggcaagcggt
       61 ggtccaagta ttttgaaaga actttcaaat gaacagattg ctatggacat cgaagatgcc
      121 atcagattga atgctgcagc agtggcagta caggtattta ttggcggcga atacgaaacc
      181 caatcagtcc ataatatgac gaaactggtg gatatgggcc agagatatgg aattggggta
      241 cttggtgtca ctgctgttgg taaagaactg gtaagagacg caaaatacat gcgtcttgcc
      301 tgcagaattt gcgcggaact cggtgcaacc tatgtgaaaa cttactatgt tgagccagat
      361 tttgaaacag tcactgcatc atgtccagtg cctattgtga tagcaggtgg caaaaaaatt
      421 ccagaatttg atgccttgaa gatggcttac aatgcaattc aacagggagc atgtggagtg
      481 gatatgggaa gaaacatctt tcaggcagaa gatcccgtgg caatgataca ggcagtcagg
      541 atggttgtgc acaaaaacgc aaaaccagaa gaagcgtacc agtattacaa agaaaaaatt
      601 tcacagaaac aaaaagggta atagtcgatg acaacatcgt ttcatcacta tgtgaggtca
      661 ttaacgccaa tgttgagtgc aggaatactt gcagcagatg tgatgcacat tggagaagag
      721 attgaaaaac ttgacgggac aggggtgaaa atgttgcatt ttgatgtgat ggatggcgtg
      781 ttctgtccta tgataactac tggtccttgt tttataaaag gggtaaaaac caaatatctc
      841 aaagatgtcc atttgatggt tcaaaatccg ataaccaaaa tttcacagtt tgcagaagca
      901 ggcgctgata tgattacagt gcatcttgaa tcatgccctg accatattca cgctctgatt
      961 caggcaataa aaaaactgaa aaatgttaac tctgttgaga gagaaattat cgcaggcgtg
     1021 gcaattaatc caggtactcc ggtcgattca atcaaaaacg ttcttgacgt ggtcgatatg
     1081 atcacaattc ttgctgtcaa tcctggatgg caggggcaat cctttattaa aacaacagca
     1141 aaaaaactgc aacaggcaat ttcactcatc aatgagtcag aaagagacat tctcctgtgt
     1201 atagatggtg gagtgacaaa agacaacatt catgacattg ctcagatggc acctgatatt
     1261 gttgttgcag gaagcgcaat ttttgatggc aaagaccccc aagccaatgc gcgatttctt
     1321 gtggagacat taaaaaaggg aaaagcaaag gaaattattc atgaaacctg aacctgaatg
     1381 cacatatgac ctgaaagaca gggttgcaat ggttactggt gctgctggtg cgattggagc
     1441 aggaatctgt aggggattac ttaaatcaaa ctgcagggtg gcagcgacag acctgccagg
     1501 agaaaaactt gataattttg ttaaagagta tcaggaagtt ttcaaagaaa aaatcattgg
     1561 cattcccatg gatgtgacag atcccgaaag tgtttctact ggaataaaaa aaattgtaga
     1621 aattttcggt tgccttgata tcgcagtgat taatgcaggt attgcgcatg tctcatcatt
     1681 gaaggatatg gacattgaag aatttcaaaa acttgaaaag gtgaatgtag aaggcaccct
     1741 tcttatacta aaagaacttg caaggtattt tgaaaaccag aaaacaggcg gcgacattat
     1801 attgatttcc acaaaaaatg tatttgcccc aggtgcctcg tttggcgcat acagcgcaac
     1861 aaaggcagca gcacatcaac ttgcccgaat cgcaagtatt gaatttgcac ccctgggagt
     1921 cagagtaaat atgatagcac ctgatgctgt gttttctgac ggcgcaagaa ggtcagggct
     1981 gtgggaaaaa ataggtcctg acaggatgaa agcacggggg cttgatgaaa agggtcttga
     2041 ggaatattat agaaacagaa atcttttgaa ggcaaaaatc acagcagacc atgtagcaaa
     2101 tgcagtacta ttttttgctt cccaccagac accaaccaca ggtgcaacaa
//
LOCUS       DAORYU010000250         2135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_34746_length_2135_cov_3.865865, whole genome shotgun sequence.
ACCESSION   DAORYU010000250 DAORYU010000000
VERSION     DAORYU010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34746_length_2135_cov_3.865865"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="PLW07_06880"
     CDS             complement(<1..571)
                     /locus_tag="PLW07_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOL50290.1"
                     /translation="MNKESMSLFNLDGMNAVVTGGAGVLGFSICEGLAGAGASVAIAD
                     MKNFKEAAEQLNEKGFDAEGFYIDVLKKDKIRECAKEILQRYKKVDILVNCAGGNLKE
                     ATTSPELSFFDLPLDALEKVVALNLFGGAILPSQVFGREMVKNPGGGSIINISSMNAF
                     RPLTRIPGYSAAKAAVSNFTQWLAVHFAME"
     gene            complement(581..1360)
                     /locus_tag="PLW07_06885"
     CDS             complement(581..1360)
                     /locus_tag="PLW07_06885"
                     /inference="COORDINATES: protein motif:HMM:NF016826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HOL50291.1"
                     /translation="MSMRFSIAKKHGFEKKIVSPENSDLKFLSFSYLFSTSEVALEEN
                     TENEEWLWVIMSGKITIRIGDVSFCMERNGVFDDKPVSLYIPADTRYAVKAETGSEVV
                     AVSAPSSGNFSPVFIPPDEIKLQRAGKSNYQRSIFNIMPQNFPASKIIAGETIHDTGH
                     WSCFPPHKHDEEKTPEESKHEELYFFKCEPEKTGFGMMRVYDETQDVAFPILPNDLIT
                     IPKGYHPVSVIPEHRLYYFWVLAGEKRPFQRHTHPDYLMYE"
     gene            complement(1422..1937)
                     /locus_tag="PLW07_06890"
     CDS             complement(1422..1937)
                     /locus_tag="PLW07_06890"
                     /inference="COORDINATES: protein motif:HMM:NF021610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HOL50292.1"
                     /translation="MPVLRIIYKRQDVARFISVNYIGKIFERAMRRLQVDLEFSHGYS
                     RRVKMSLGPPIAVGISGINEIVDIYVVNMDISADFIKKNLNEILPNGLNIEECHYLKD
                     NEKSPPEIKKARYIIKRFSNTIGDVPESWSIIGRTKDMIEIEVCIEKFRHRVLFDVFG
                     TTCLIERKLIF"
     gene            complement(1925..>2135)
                     /locus_tag="PLW07_06895"
     CDS             complement(1925..>2135)
                     /locus_tag="PLW07_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOL50293.1"
                     /translation="AAKQMDEKKRKLLYDRWQELICENQVMIFTATQAVLYAVKDKFG
                     NLKPTVHGGLFHNIEELYIKNQCQY"
BASE COUNT          635 a          467 c          339 g          694 t
ORIGIN      
        1 attccattgc aaaatgaaca gccagccatt gcgtaaaatt acttactgct gcttttgctg
       61 cagaataacc aggaatcctt gtaaggggtc tgaacgcatt catggaagat atgttgatga
      121 tggaaccacc gccgggattt tttaccattt cccttccaaa cacctgcgat ggaagtatcg
      181 cccctccaaa aagattcagc gccacaacct tttcaagcgc atcaagagga aggtcaaaaa
      241 aacttaattc aggagatgtt gtcgcctcct ttaagtttcc tccagcgcaa tttacaagaa
      301 tatcaacttt cttatatctt tgtagaattt cttttgcaca ttccctaatt ttatcttttt
      361 tcagtacatc aatgtaaaac ccttctgcat caaatccttt ttcatttaac tgttctgctg
      421 cttctttgaa atttttcata tcagcaatag caacagacgc acctgcacca gccagtccct
      481 cacatatact gaatccaagt accccggcac ctcctgttac cacagcattc attccgtcaa
      541 gattgaacaa actcatactt tccttattca tgcagcctcc ttattcatac attagataat
      601 caggatgcgt gtgtctttga aatggacgtt tttcaccagc aagtacccag aaataataaa
      661 gacgatgttc tggaatcaca ctcactggat gatacccctt tggaattgta attaaatcat
      721 ttggaagaat cggaaatgca acatcctgtg tttcatcata aacacgcatc ataccaaaac
      781 ctgttttttc tggttcacat ttgaaaaaat atagttcttc atgtttgctt tcttccggcg
      841 ttttttcttc atcgtgttta tgtggaggaa aacaggacca gtgtccagta tcatgaattg
      901 tttcacccgc tatgattttg gaagcaggaa aattctgggg catgatatta aaaattgaac
      961 gctgataatt ggattttcct gctctttgaa gctttatttc gtcaggcgga ataaaaacag
     1021 gagaaaaatt tccactggaa ggagcagaaa ccgcaaccac ttctgaaccg gtttcagctt
     1081 tcacagcata tctggtatca gcaggaatgt ataaagaaac tggtttatca tcaaaaaccc
     1141 cgttcctttc catgcagaac gaaacatctc ctatcctgat ggtaattttt cctgacatga
     1201 taacccacaa ccattcctca ttttcagtat tttcttccaa tgcaacttct gaagttgaga
     1261 acaaataact gaaggagaga aatttaaggt cagaattctc tggtgaaaca atttttttct
     1321 caaaaccatg cttttttgca attgaaaatc tcattgacat cgcatctcct ggattatcgg
     1381 gttataagaa caaactgttt tgtaaattat actgaatttc ttcaaaaaat caattttctt
     1441 tctatcagac aggttgtgcc aaacacatca aaaagaaccc tgtgccggaa tttttcaatg
     1501 cagacctcaa tttcaatcat atctttggtt ctaccaataa tactccatga ttccggaaca
     1561 tcaccaatag tattggaaaa acgtttgata atatatcttg ccttcttgat ttcaggaggg
     1621 gatttttcat tatcttttag ataatgacat tcttcaatat tcaagccatt cggcagaatc
     1681 tcatttaaat tttttttgat aaagtcagcc gaaatatcca tattcacaac atatatatca
     1741 acaatctcat tgataccaga aatcccaact gcaataggtg ggccaaggct cattttgact
     1801 cttcgactgt aaccatgaga aaattccagg tctacctgta atcttctcat tgccctttca
     1861 aagattttac caatgtaatt gacagatata aacctggcaa catcctgcct tttgtaaatg
     1921 attctcaata ctggcattgg ttttttatgt agagttcttc tatattatga aacagcccgc
     1981 catgtactgt gggcttcaaa tttccgaatt tgtctttgac tgcgtacaga actgcctgtg
     2041 tggcggtaaa aatcattacc tgattttcac atatcaattc ctgccacctg tcataaagaa
     2101 gttttctttt tttttcgtcc atctgctttg ccgcg
//
LOCUS       DAORYU010000251         2128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_34899_length_2128_cov_4.901110, whole genome shotgun sequence.
ACCESSION   DAORYU010000251 DAORYU010000000
VERSION     DAORYU010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34899_length_2128_cov_4.901110"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1155)
                     /locus_tag="PLW07_06900"
     CDS             complement(<1..1155)
                     /locus_tag="PLW07_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50294.1"
                     /translation="MEKIINKLSSFNFEERIEALEKIVYFIKTGDIKKHEKQFPYDNM
                     HIHSFHSFNYNNWSPSRIILEAYRHGLKHIGIVDFDTIAATEEAHIASKKIGIPTLTG
                     LETRVHVSFFADRVINSPGEPGIYYINGLGFTKFPQPGTEAERIYKNLHFIASRRNKL
                     VVDKLNQFLTPVKIDYEKDVLPLSPSHNPTERHIIKAYVEKAESILEKGESKFWADIL
                     KISPENVAILKEQKPGDFMEKLRSILVKNGGPGYVQPEPETFPEMKDFINMTQDSGAI
                     VVGNWLDGTTEGEKNPEEFLEFMIASGIKVMNIIPERNWNIQNPEEKTKKVKNLDVFL
                     ATCRGKNIPVICGTEMNKYGQPFIDNYDTPELRKHLEWFQKSVIQLFEMAN"
     gene            complement(1139..1801)
                     /locus_tag="PLW07_06905"
     CDS             complement(1139..1801)
                     /locus_tag="PLW07_06905"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HOL50295.1"
                     /translation="MITDSLDEYLKETRYCDIHSQEIVNFCKNIESDDAIDIAGFIFD
                     FVRDNIKYRFDYPSVRASETLRKKTGNCFNKANLQIALLRKTGIPAGYGVYLVRKEIL
                     KPILPPDIYQMVNQPTVHVFTKLFIQNRWVSLDATVDLEMFQCFYSSSGTWTHNCWNR
                     QSDISLDKGFVVEDQGVYANIDLYLSQVPKFWTDQLIQKANCFIEESIKNMREGKNGK
                     NH"
     gene            1855..>2128
                     /locus_tag="PLW07_06910"
     CDS             1855..>2128
                     /locus_tag="PLW07_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50296.1"
                     /translation="MSKIYVIITAIVFACGCAHYSAYENFQRDKKARLEFAKKHNELA
                     PEVRQAIVEGKILPGMDQKLIRDLFGEPDETYLSETGMFEMWYYENF"
BASE COUNT          574 a          395 c          404 g          755 t
ORIGIN      
        1 gtttgccatt tcaaacagtt gaataacgct tttctgaaac cattcaagat gttttcttaa
       61 ttcaggagta tcataattgt caatgaatgg ttgaccatat ttgttcattt ctgtgccgca
      121 tattaccggt atattttttc ccctgcaggt tgcaagaaat acgtcgaggt tcttcacctt
      181 ttttgtcttt tcttcaggat tttgtatgtt ccagtttctt tcaggaataa tattcattac
      241 ttttattcca gacgcaatca taaactcaag aaattcttca ggattttttt ctccttctgt
      301 tgttccatca agccagtttc caaccacaat tgctccgcta tcctgtgtca tgttgataaa
      361 atctttcatc tcaggaaatg tttcaggctc tggctgaaca tatcctggtc caccattttt
      421 taccagtatc gaccgcagtt tttccataaa atcgcctggt ttctgttctt tcagtattgc
      481 tacattttca ggtgatattt ttagaatatc agcccagaat ttactctccc ctttttccaa
      541 aatgctttct gctttttcaa catatgcctt aatgatatgt ctttcagtgg gattgtgtga
      601 agggctcagt ggaagcacat ctttttcata atcaatcttg accggtgtta gaaactgatt
      661 gagcttatca actacaagtt tgttccttct gctggcaata aaatgtaaat ttttatatat
      721 tctttctgct tctgtacctg gttgaggaaa ttttgtaaaa ccgagtccat tgatatagta
      781 gattccaggt tcaccgggtg aattaatcac cctgtctgca aaaaacgaaa catggaccct
      841 tgtttcaagt ccagtaaggg tggggatacc aatttttttt gacgcaatgt gtgcttcttc
      901 agtggctgcg atggtatcaa aatcaacaat tccaatgtgt ttcagtccgt gtctgtatgc
      961 ttccagaata attcttgatg gagaccagtt gttgtaattg aacgagtgga atgagtggat
     1021 gtgcatgttg tcataaggaa actgtttttc atgttttttt atgtcgccag ttttgataaa
     1081 gtacacaatt ttttccagtg cctctatcct ttcttcaaaa ttgaatgaag acagtttatt
     1141 aatgattttt tccatttttt ccctctcgca tgtttttaat ggattcttct ataaaacagt
     1201 tcgccttctg tatgagctgg tctgtccaga attttggaac ctgtgataga taaaggtcta
     1261 tgtttgcata aacgccctgg tcttctacaa caaatccttt gtcaagagat atgtctgatt
     1321 gcctgttcca gcagttatga gtccatgtgc ctgaactgct gtaaaaacac tgaaacattt
     1381 caaggtctac agtggcatct aaactgaccc atctattttg tatgaagagt ttcgtgaaaa
     1441 catgaacagt tggctggttg accatctggt aaatatcagg tggaagaata ggtttcaaaa
     1501 tctccttgcg aacaagatat acaccgtagc cagcagggat acctgttttt cttaatagcg
     1561 ctatttgaag atttgccttg ttgaaacaat ttcctgtttt tttgcgcagg gtttctgatg
     1621 ccctgacaga tggatagtca aaacgatatt tgatgttgtc ccgcacaaag tcaaagataa
     1681 atccagcgat gtcaatcgca tcatcagatt ctatgttttt gcagaaatta acaatctcct
     1741 gcgaatgtat atcgcaatat cttgtttctt tcaaatattc atccagagaa tcagtaatca
     1801 tttttcttgt gtggtataat gttttttgaa taatatatta ctccaggagg tcgtatgagt
     1861 aaaatctatg tgattatcac tgcgattgtt tttgcatgcg gttgcgctca ttattctgcg
     1921 tatgaaaatt tccagcgcga taaaaaggca cggcttgaat ttgctaagaa gcacaatgaa
     1981 cttgctcctg aagttagaca ggcaatcgta gaaggaaaga ttttaccggg aatggaccag
     2041 aagttgataa gggatctttt tggggaacca gatgagacat atctttcaga aacaggtatg
     2101 tttgaaatgt ggtactatga aaatttcg
//
LOCUS       DAORYU010000252         2089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_35846_length_2089_cov_5.663225, whole genome shotgun sequence.
ACCESSION   DAORYU010000252 DAORYU010000000
VERSION     DAORYU010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35846_length_2089_cov_5.663225"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="PLW07_06915"
     CDS             complement(<1..756)
                     /locus_tag="PLW07_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50297.1"
                     /translation="MKSYKSMTRNYKSLIPSKKEIGIFRRKIIAWFEKNGRVYPWRQE
                     RDPFRVLVAEMMLRRTKADQVVPVYKQFLKEFPDVDTLAEAKQEAIEKILYPLGLKWR
                     IPAFGLMAQEVREKYHSKIPEDRKKLMSLPGVGEYVTGAVLSIAYGKNEWAVDSNIVR
                     IFKLILTKQKGEKMKKILNNLYIGELITDIKKYSNLQSTQTKTSLSWGDCCGFMMEEE
                     REIVKRRFLPYLKIIIEHFDKFKDARKRAIEIAI"
     gene            complement(743..>2089)
                     /locus_tag="PLW07_06920"
     CDS             complement(743..>2089)
                     /locus_tag="PLW07_06920"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HOL50298.1"
                     /translation="QDKFSAIDLFAGPGGLSEGFSQAGFDIICSVEKDRFATQTLRTR
                     VMFRTLKEIGKLPLYWNYVRGNKTFEDIFNLCPEVRYEIESRVLQETISDRSRERILK
                     AIEKLLKDSGAANLTVLLGGPPCQLYSIIGRARYANMKDKFYRDPRRRLFEHYIFFLQ
                     KLQPDIFVFENVFGITSSTLKRRGLLEILFEEFDKCGYVIPGVENGGVNGNGHLLNSL
                     YFGVPQRRKRLILIGYRSDLNGKCRMNEIYKIIRKKYPATKNSYPVVQDAIGDLPFIR
                     AGEGNDRWYGFYPESVKVSEYASILRNESEGVLNHRARAHMKEDLERYRYFIEHSNNG
                     RKADLNLLKKERPDLLPAHRNLNGFLDRFKVQIWDEPSSTITSHLAKDGHYYIHPDIN
                     QLRSFTVREAARCQSFPDNYLFEGPRTEQFRQVGNAVPPMMAFKIAKCLKEILEEL"
BASE COUNT          553 a          474 c          356 g          706 t
ORIGIN      
        1 tattgctatt tcaattgctc tttttcttgc atctttaaat ttatcaaaat gttcaattat
       61 gatttttagg taaggaagaa atcttctctt gacgatttcc cgctcttctt ccatcataaa
      121 accacaacaa tccccccaag agagcgatgt cttcgtttgg gtggattgta aatttgaata
      181 cttttttata tcggtgatta gttcaccgat ataaagatta ttaagtattt ttttcatctt
      241 ttcccccttt tgtttagtca atatcaattt aaaaattctt acaatgttgc tatccacagc
      301 ccactcattt ttcccgtatg caatggataa taccgctcct gttacatatt cacccactcc
      361 gggcagagac atgagttttt ttctgtcctc cggtattttt gagtggtatt tctcgcgcac
      421 ttcctgtgcc ataagtccaa aagctggtat gcgccatttc aagccaagag ggtaaagaat
      481 tttttcaata gcttcttgtt ttgcttccgc aagtgtatca acatcgggaa actcctttaa
      541 aaactgctta tatacaggaa ctacctgatc cgcctttgtt cgcctcagca tcatctccgc
      601 aacaagaacc cggaatgggt ccctttcctg tctccaagga taaacccttc catttttttc
      661 aaaccacgct ataatctttc ttctgaaaat tcctatttcc ttctttgagg ggatcagtga
      721 tttataattt ctggtcatgg acttataact cttcaagaat ctccttcaga cattttgcta
      781 ttttgaatgc catcatcggt ggcactgcat taccaacctg acggaactgt tcggttctcg
      841 gaccctcaaa aagataattg tccgggaaag actgacaccg tgctgcttcc cgtactgtaa
      901 atgagcgcag ttgatttata tctggatgaa tgtaataatg accgtccttt gcaagatgtg
      961 atgtaatggt actggatggt tcgtcccata tctgaacttt gaacctgtcg aggaaaccat
     1021 taagatttct gtgcgcgggg agtagatcgg gacgttcctt tttaagtaaa ttcaaatcag
     1081 ccttccttcc attgttactg tgttcaataa aatacctgta tcgctcaagg tcttccttca
     1141 tgtgtgctct tgctctgtgg ttaaggaccc cttcactttc atttctgaga atagatgcgt
     1201 attcagaaac ctttactgac tccgggtaaa agccatacca cctgtcattg ccttctcctg
     1261 ccctgataaa tggaagatct ccaatggcat cttgcaccac aggataacta tttttcgttg
     1321 caggatattt ttttctgata attttataaa tttcattcat tctgcacttc ccattgaggt
     1381 cgcttctgta accaatcagt atcagtcttt ttcttctctg cgggactccg aaataaaggc
     1441 tattcaaaag atgaccattt ccattgacac ctccattttc tactcctgga attacatatc
     1501 cgcatttatc aaattcctca aagagaattt ccagaagtcc ccttcttttt aaagttgatg
     1561 atgttatgcc gaaaacattt tcaaaaacaa aaatatcggg ctgaagtttc tggagaaaaa
     1621 aaatgtagtg ttcaaataat cttcttcttg ggtcacggta aaatttatcc ttcatatttg
     1681 catatcttgc ccttcctata atggaataaa gctgacacgg aggtcccccg agaagaacgg
     1741 taaggtttgc ggcacctgaa tccttcaata gtttctcaat cgctttcagg attctctccc
     1801 gtgatctatc agaaattgtt tcttgaagaa cccttgattc aatctcatat cgtacttccg
     1861 gacacagatt gaatatatct tcaaaggttt tatttcccct gacataattc cagtaaagtg
     1921 gaagttttcc gatctctttc agtgttctga acataactct cgtgcgaagt gtttgtgtag
     1981 cgaatctgtc tttttccacc gaacaaataa tatcaaaacc tgcctgcgaa aatccttcac
     2041 tcagaccacc gggacctgcg aacaggtcta tggcagaaaa cttgtcctg
//
LOCUS       DAORYU010000253         2083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_36005_length_2083_cov_4.211045, whole genome shotgun sequence.
ACCESSION   DAORYU010000253 DAORYU010000000
VERSION     DAORYU010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36005_length_2083_cov_4.211045"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="PLW07_06925"
     CDS             <1..301
                     /locus_tag="PLW07_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HOL50299.1"
                     /translation="ACIEAKAMLGDDLPTVAVKKGISRESAILLSPEETREMLIEGAK
                     EAVKKMPALKPYKVKFPIKLRIKRLGPEGATLENPYFSEKEVEVKNGLDIISGSV"
     gene            324..1202
                     /locus_tag="PLW07_06930"
     CDS             324..1202
                     /locus_tag="PLW07_06930"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOL50300.1"
                     /translation="MFIDIHVHFRKYQTILRNGKYTYSTPEQLLKRYDELGIEKAVVL
                     PGGGPECRIQAQSEEEVLEICRKYPDRFIPFCNVDPRSVTNSPDAPLDELLIYYKQQG
                     CKGIGEVTANLPFLNPLVQNLFRHAEKVGLPVTFHIAAQIGGAYGLYDDPGLPQLERT
                     LQRFPNLCFLGHSQPFWAEMAQLETPADRYRYPSYPVKKEGVVPKLFRRYKNLLGDLS
                     AYSGFNAISRDIDYGVEFLNEFQDRLFFGTDICPPDTPTPLVDFLKKLRDEGRISKSV
                     FNKIARENAVKLLGLT"
     gene            1236..1964
                     /locus_tag="PLW07_06935"
     CDS             1236..1964
                     /locus_tag="PLW07_06935"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOL50301.1"
                     /translation="MIYLIFSLASFVQALGGFGAGLFAVPLLAMSYEPRFIIPPFALT
                     VFVLNLVIISKVKNNVNCKRIIFIAAGSFAGLPFGVFALKYVNQDVIRFSISLITFVL
                     GLLFLFGLKPKIKESKTTFIISGIVSGILSGAAAMGGPPLIFILMSMGFPKDNFRATL
                     LGCFLFNGIYANTLYFVNGLFSLSNLRISLLGLVPALAGTILGIKTKNMLTEEQFNRA
                     TLIIVIIIGIIGIIRALCLLKTCF"
BASE COUNT          631 a          360 c          436 g          656 t
ORIGIN      
        1 cgcatgcata gaagcaaagg caatgcttgg agatgattta ccgacagttg cagtgaaaaa
       61 gggtataagt agagaatccg caattttgct ttcgcctgaa gaaacaaggg aaatgttgat
      121 tgaaggtgca aaggaagccg ttaaaaagat gccggctctt aaaccataca aggttaaatt
      181 tccaataaaa ctgcgtataa aacgacttgg accagaagga gcaacactgg agaatcctta
      241 tttttctgaa aaagaagtgg aagtcaaaaa tggtctcgat attataagtg gtagtgtttg
      301 atttttcaaa agaggagaaa ataatgttca tagatattca tgtgcatttt agaaagtacc
      361 agacaatttt aaggaatggt aaatatactt attcaacacc agagcaactg ttgaaaagat
      421 acgatgaact cgggatagaa aaggctgtgg tattaccagg tggtggtcca gaatgtagaa
      481 ttcaggccca gagcgaagaa gaagtacttg agatttgcag aaaatatcct gacagattca
      541 tcccattttg caatgtggat cctcgttcag taacaaattc acctgatgct ccacttgatg
      601 aactattgat ttactacaaa cagcagggtt gcaaagggat aggcgaggtc acggcaaatt
      661 taccctttct caatcctctg gtgcagaatc tgttcaggca tgcagaaaaa gtggggctgc
      721 ctgttacctt tcatatcgct gctcagatag gcggtgcgta tggactttat gatgatccgg
      781 ggctccctca attggaaaga actcttcagc gatttcccaa tctttgtttt ctcggacatt
      841 cccaaccttt ctgggcagaa atggctcaac tcgaaacacc tgctgacaga tacagatatc
      901 cgtcgtatcc tgtgaaaaag gaaggtgttg ttccaaaact gttcaggcgg tacaaaaatc
      961 ttcttggaga cctttctgca tatagtggtt ttaatgcaat atcacgtgat attgattatg
     1021 gagtggagtt tttaaatgaa tttcaggaca ggctattttt tggtactgat atctgtccac
     1081 ctgatactcc aaccccgctt gttgattttt tgaagaaact gagagatgaa ggtagaatat
     1141 caaaatctgt gtttaacaaa attgcccgtg agaatgcagt aaaactactt ggtctaacct
     1201 gaaatctgag ggattgattc tactagttgt ttttgatgat ttatctcatt ttttcccttg
     1261 cttcatttgt tcaggcgctt ggaggttttg gcgcagggct ttttgctgtc cctctacttg
     1321 ctatgtctta tgaacccaga tttattatac caccttttgc actcacagta tttgttttga
     1381 accttgttat tatttcaaaa gtgaagaata acgtaaactg taaaagaata atttttattg
     1441 ctgcagggtc gtttgcgggt ttaccttttg gggtttttgc tttgaaatat gtaaatcaag
     1501 atgttataag gttttcaata tcattaatca catttgtact ggggttgtta tttctgttcg
     1561 gtttaaaacc aaagataaaa gaatcaaaaa ccacgttcat catttcaggt attgttagtg
     1621 gtattttatc cggagcagcg gcaatgggtg gaccaccgct tatttttatt ttaatgtcaa
     1681 tgggatttcc aaaagataat tttagagcaa cacttcttgg gtgttttttg tttaatggga
     1741 tttatgcgaa caccctttat tttgtcaatg gactattcag tttaagtaat ctcaggatat
     1801 cactcttggg tcttgtgcca gcactcgcag gaacaatact tggcataaaa acaaaaaata
     1861 tgcttacaga agaacagttc aatcgcgcca ctctcattat agtgataatc atcggaatta
     1921 ttggtatcat tcgtgcgcta tgtcttttaa aaacctgttt ttgacatacc tctatatcaa
     1981 aagtaaaata gtcctgctat ggaaaaacag aaaaaaattg aaatttatga caccacattg
     2041 agagatggtg cccaggggga atcgattttt tttacacttc atg
//
LOCUS       DAORYU010000254         2075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_36205_length_2075_cov_3.847030, whole genome shotgun sequence.
ACCESSION   DAORYU010000254 DAORYU010000000
VERSION     DAORYU010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36205_length_2075_cov_3.847030"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /gene="tsf"
                     /locus_tag="PLW07_06940"
     CDS             <1..239
                     /gene="tsf"
                     /locus_tag="PLW07_06940"
                     /inference="COORDINATES: protein motif:HMM:NF013085.2"
                     /note="EF-Ts; functions during elongation stage of protein
                     translation; forms a dimer; associates with EF-Tu-GDP
                     complex and promotes exchange of GDP to GTP resulting in
                     regeneration of the active form of EF-Tu; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HOL50302.1"
                     /translation="LQIAATSPEYIKKDDIPESVIEEKKKVYSDIEQFYREKVLFCQP
                     FIKDDSITIEEYVKSKIAKLGENIRVNRFTRYEI"
     gene            239..625
                     /locus_tag="PLW07_06945"
     CDS             239..625
                     /locus_tag="PLW07_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HOL50303.1"
                     /translation="MKKIVFEIGTVRIEGTLNSSNTAAKIYSTLPIKGKANLWGKEIY
                     FSVPVHLPLEDGKEIVNIGDIAYWPEGPAVCIFFGPTPISRGDEIRPYSKVTLIGQVD
                     GNFIKDLYNIKDGDKIEMKPLTSGGD"
     gene            625..948
                     /locus_tag="PLW07_06950"
     CDS             625..948
                     /locus_tag="PLW07_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50304.1"
                     /translation="MNSVSAGVYYEMGYLLALAVIGLSFLGFMLAMMMDEINKKKGIV
                     MYVLSIILLLLGGYYYYTVGQWQKLQGGPSINKLNYYLHVYQEQPASQQQILPLSPQL
                     PQNTQ"
     gene            991..>2075
                     /locus_tag="PLW07_06955"
     CDS             991..>2075
                     /locus_tag="PLW07_06955"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HOL50305.1"
                     /translation="MFILCGCTHVPQFIPTPVELDNQKEKFFLDKGSVYLLDEADVQV
                     FTSGASVTTVHQKILITGEKGKQHGTLQIPFDAEIQKIEILKAITITEENKILAVKKS
                     DIRIITPAELSSYSVLYPGIKVCTVTFPSVNIGCIVEYVYKIKTKESRMPGEFWDGFF
                     FQGTEVFVVSRYRITIPANMELYTYPFSVALKSKTKKGNLIVYEWEKMNVPAITPEKM
                     MPPLEEVVPKLYVTTIKNWDKIGRWFFALAKDATIPDSTVKSITESITRDTRNNEEKI
                     KAIYHFICKNIRYIGLELGIHGFKPHAAKDVIKLGYGDCKDKAALMVAMLKSAGIQSY
                     IALVNTERQIDENIPFPGQFNHAIVAIG"
BASE COUNT          713 a          351 c          418 g          593 t
ORIGIN      
        1 gccttcaaat cgctgcgact tcgcctgaat atattaaaaa agatgacatt cctgaaagcg
       61 ttattgagga aaaaaagaag gtttatagcg atatagagca attctatagg gaaaaagttc
      121 tgttctgcca accattcatc aaagacgata gtattaccat tgaagagtat gtgaaaagca
      181 aaattgcaaa acttggcgaa aatataagag tcaatagatt cacccgttat gaaatatgat
      241 gaaaaaaatt gtttttgaaa ttggtacggt tcggatcgaa ggcacactca attcaagtaa
      301 tactgcagca aagatttatt ccacactacc aataaaaggt aaggcgaacc tgtggggcaa
      361 ggaaatatac ttttctgtac cggtgcactt acctcttgaa gatgggaaag agattgtgaa
      421 tattggagac attgcttact ggcctgaagg accggctgtg tgcatttttt ttgggccgac
      481 accaatcagt agaggagatg aaattagacc atacagcaag gttaccttga ttggtcaggt
      541 tgatggaaat tttataaaag atttgtataa tataaaagat ggagataaaa tagaaatgaa
      601 acccttaacc agtggaggtg actgatgaat tctgtgtctg cgggcgtgta ttatgaaatg
      661 ggatatttgc ttgcccttgc agtaatcgga ttaagctttc ttggttttat gctggcgatg
      721 atgatggacg agataaacaa gaaaaagggc attgtaatgt atgttctttc tataattctt
      781 ctgcttcttg gcggttatta ttactacaca gtgggacaat ggcaaaaatt acagggtgga
      841 ccaagtatta acaagctcaa ctattattta cacgtttatc aggagcaacc agcttcacaa
      901 cagcaaattc ttccgttatc tccacaacta ccacaaaaca ctcagtaagg aaatttttca
      961 atgttgcggt actggtggca ttttttattt gtgtttatac tttgcggatg tacacatgtt
     1021 ccgcaattta ttcctacccc tgtagaatta gacaatcaaa aagaaaaatt tttcttagac
     1081 aaaggttctg tatacttgct cgatgaggcg gatgtccagg tcttcacctc aggtgcttca
     1141 gtaacaactg tccatcaaaa aattctcatc acgggtgaaa aaggcaagca acatggtaca
     1201 cttcaaatac cctttgatgc tgaaattcaa aagatagaaa tcctgaaagc aataacgatt
     1261 actgaggaaa ataaaatact tgcagtaaaa aagtcagaca ttagaatcat tacacctgct
     1321 gaattatcat cgtactcagt gctttatcct ggtataaagg tttgtactgt gacttttcca
     1381 tctgtcaata ttggttgcat agttgaatat gtctataaaa ttaaaacaaa agaatccagg
     1441 atgccaggtg aattctggga cggatttttc tttcagggta cagaggtctt tgttgtttcg
     1501 cgatacagaa ttacgattcc ggctaacatg gagttatata catatccatt ctcagttgca
     1561 ttaaaatcaa aaacaaaaaa gggaaacttg attgtatacg aatgggaaaa aatgaatgta
     1621 ccggcaatta cgccggaaaa aatgatgcct cctttagaag aagtagtacc gaaactttat
     1681 gtcaccacaa tcaaaaactg ggataagatt ggaagatggt tttttgccct cgcaaaagat
     1741 gcaactatcc ctgactctac tgtaaaatct attactgaaa gcataacgag ggataccaga
     1801 aataatgaag aaaaaataaa agcaatatat cacttcatct gtaaaaacat cagatatatc
     1861 gggttagaac tgggtattca cggattcaaa ccacatgcag caaaagatgt tattaaactg
     1921 gggtatggtg actgtaaaga taaagctgca ttaatggttg caatgctgaa atcagctggt
     1981 atccagtctt atatagccct tgtcaacaca gaaaggcaaa ttgatgaaaa tataccgttt
     2041 cctggacaat ttaaccatgc aattgttgct atagg
//
LOCUS       DAORYU010000255         2069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_36355_length_2069_cov_3.299901, whole genome shotgun sequence.
ACCESSION   DAORYU010000255 DAORYU010000000
VERSION     DAORYU010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36355_length_2069_cov_3.299901"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PLW07_06960"
     CDS             <1..199
                     /locus_tag="PLW07_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HOL50306.1"
                     /translation="VFGIVVPTTNQVSNVIRICETLPVINLEVCEILFRKYRINPERL
                     RPFDVMVGHTAYLIAGRKIKT"
     gene            234..>2069
                     /locus_tag="PLW07_06965"
     CDS             234..>2069
                     /locus_tag="PLW07_06965"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOL50307.1"
                     /translation="MNRTIYRIIFSMVFFVASVYGIDFNFVGQFENDGFQHAVKADFN
                     RDGKIDIAAVNSTGDVLVFLGKYESPFFDEPIITSIGGYLTYIIAEDFNRDGKMDIAV
                     TSYQYPERMSVLLGNGDGTFQGPAHYFAGAYPKGIVSADFNRDGIPDIAVVSYNGAFY
                     CYLGDGEGNFNLTSSNTTSYVSLYLTAADFDNDGIMDVAVSGYFNSNISVFRGKGNGT
                     FYDPSSVSISSSWFITTGDFNRDGKADLAVTSRSGPGQVAVLMGNGDCTFQTPVYYDT
                     GGEPWCIDVNDFDSDGISDLAVVNQYSKTLTVLKGKNDGTFQSSFNYGCPSGLYAVVS
                     GDFDGDGKLDIALILYNRMVILENSTEFKESGIFSMPVHYQVGVDPRNVIAGDFNNDG
                     IFDLAVANEGSASVSILKGKPDGTFSDAINYQFEPALGYEFRPYNLVAADFNRDGKPD
                     IAVANSNGMTYSIMTGNGDGTFSVNTFFIGIGGQHFSIVSGDFNRDGKPDIAIISDNY
                     PNSGVIVLSGNGDGTFGNQLFYRLVNASRVSLCDFNKDGITDMAVIARYTNGVFLFTG
                     NGDGAFTSAGSISSINSPTGIVFADFNTDGNPDIAVCDWSEDNLFS"
BASE COUNT          586 a          322 c          472 g          689 t
ORIGIN      
        1 agtatttgga atagtcgtac caacaacaaa tcaggtttca aatgtaattc ggatatgcga
       61 gaccctgcct gttattaatc ttgaagtgtg cgagatactg ttcaggaagt accgtattaa
      121 tccagaaaga ttaagacctt ttgatgtgat ggttggtcac accgcatatt tgattgcagg
      181 tagaaaaatt aaaacatagt atcttcatgt tataatgata aaaaggagaa aacatgaata
      241 gaacgattta ccgaataatc ttttctatgg tattttttgt ggcgtctgta tatggcatag
      301 atttcaactt tgtgggtcaa tttgaaaatg acgggttcca acatgctgtg aaagcggact
      361 tcaatagaga cggaaagatt gatattgctg ctgtgaattc cactggagat gtgcttgttt
      421 tccttggaaa atatgaaagc cctttttttg atgaaccgat tatcaccagt attggtggtt
      481 atttaactta tatcattgca gaagacttca atcgagatgg caagatggat attgctgtta
      541 caagttatca gtatcctgaa aggatgagcg tgcttttggg caatggtgat ggaacatttc
      601 aaggaccagc acattatttt gcaggtgctt atccaaaagg tattgtgtca gcagatttta
      661 acagggatgg aatacctgac attgctgttg tctcttataa tggtgcgttt tattgttatc
      721 ttggagatgg agaaggcaat ttcaatctta cttcttcaaa taccaccagt tatgtgtctt
      781 tgtatttaac tgcagcagat tttgataatg atggcatcat ggacgttgca gtcagcggtt
      841 attttaattc caatatcagt gtttttcgcg ggaaaggcaa tggtactttt tatgacccgt
      901 ccagtgtttc tatttcttct tcctggttca ttacaacagg tgattttaac agagatggca
      961 aagcagacct tgctgtcaca agtagaagtg ggccaggcca ggttgcagtg ttaatgggaa
     1021 atggtgattg tacctttcaa acacctgttt attatgatac aggcggagaa ccctggtgca
     1081 ttgatgtgaa tgattttgat agcgatggga taagcgacct tgctgttgtc aatcagtatt
     1141 ctaaaaccct tactgttttg aagggaaaaa atgatgggac ttttcagtca tcttttaatt
     1201 atggttgtcc tagcggcctg tatgctgttg tgtcaggtga cttcgatggc gatggtaaac
     1261 tggatattgc cttaatactt tataaccgta tggtaattct ggaaaactca actgaattca
     1321 aagaatcagg aatattttca atgcctgtcc attatcaggt cggagtagat ccaaggaatg
     1381 taattgcagg cgacttcaat aatgatggga tttttgacct tgctgttgcc aatgaaggta
     1441 gcgccagtgt ttctatttta aaagggaaac ctgatggaac attttctgat gcaataaatt
     1501 atcaatttga gccagcgctt ggttatgaat ttaggcccta caaccttgtg gctgcagatt
     1561 ttaataggga tggaaaacct gatattgctg ttgccaattc aaatggtatg acatacagca
     1621 taatgacagg caatggtgat ggaacatttt cagtaaatac attttttatc ggtattggtg
     1681 gacagcattt ttcaatagtg tcaggagatt ttaacaggga tggaaaaccg gatattgcaa
     1741 taataagcga taattatcca aatagtggag tcattgttct ttctggcaac ggagatggaa
     1801 catttggtaa tcagttgttt tatcgtcttg ttaatgcgag tcgtgtttct ttgtgcgatt
     1861 ttaacaagga tggaattact gatatggcag taattgcgag gtataccaat ggagtatttt
     1921 tatttactgg aaatggcgat ggcgctttta ccagtgccgg cagtatttct tcgataaatt
     1981 ctccaacagg cattgtgttt gctgatttca acactgatgg aaaccctgat attgctgttt
     2041 gtgattggtc tgaagacaat cttttttct
//
LOCUS       DAORYU010000256         2051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_36765_length_2051_cov_5.667836, whole genome shotgun sequence.
ACCESSION   DAORYU010000256 DAORYU010000000
VERSION     DAORYU010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36765_length_2051_cov_5.667836"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="PLW07_06970"
     CDS             <1..281
                     /locus_tag="PLW07_06970"
                     /inference="COORDINATES: protein motif:HMM:TIGR04294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50308.1"
                     /translation="KSKVDLCAKKGLIWVADAWGYYAWNYGNPPGMSSEGYSHNNGRD
                     QLPAGMNVLFFDGSVKWINDPTHKLLYTYHYNSYGTESFWLLTEKDFK"
     gene            282..1676
                     /locus_tag="PLW07_06975"
     CDS             282..1676
                     /locus_tag="PLW07_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50309.1"
                     /translation="MIKILFFDYKHIEIIEGFKRNIEPPKKYGGNPVFVSGHPLEENW
                     ISLYGSVIRKPDGCWQMWYTTMMNNKKNLVLGYAESEDGIHWKRIENDAVKINETRTH
                     FVFDREPHGAAIIYDEQETRENWKYKMICGASLSGCISVFHSRDGIHWIPAQKNPVIG
                     TNPDCPMGFLRLPSDKYVAYHRPVFGDRRIARSESWDFINWSDAKTVIDQSPLDPPQT
                     QFYGMGAASYGEYEIGTLWIYHTDHNDFGFWKMEGYQEPELVYTRTGYAWHRIEINMP
                     WIKRGEKGAWDCGQIQPASAPVFLEDEIRVYYAGTRTTHGKSLETWNTSEPRCGIGFV
                     SIKPDRFAGLVASDKGILLTRPFWSEVPQFFVNANIEGFLCVEITDVSGKPVPGFELE
                     NAIPMKGDSLYHPCRWKNNTGTSGLVNKDIRFKVVANNAKVYSLMAGSEKDVAKYRDF
                     RVTYFPFEREKNFI"
     gene            1719..>2051
                     /locus_tag="PLW07_06980"
     CDS             1719..>2051
                     /locus_tag="PLW07_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50310.1"
                     /translation="MKKILISILLFSGSLLFSDVSSFKTQFFTPVTDLVTPHITWLSP
                     YKNGKLNLLWITYRRDGGFREIIELSQRMDINFTVFTLATPDKFTPWSSYYKPVPVTD
                     EEYQKDLEE"
BASE COUNT          671 a          361 c          451 g          568 t
ORIGIN      
        1 cgaaatcgaa ggtggacctt tgcgcgaaaa aaggtctgat ctgggttgct gatgcctggg
       61 gctattacgc atggaactat ggaaaccctc ctggtatgag ttctgaaggg tacagccata
      121 ataacggaag ggaccagtta cctgcaggga tgaatgtttt gttttttgat ggttctgtga
      181 agtggataaa tgacccaacc cataaactac tttatactta ccattacaat tcatatggaa
      241 cagaaagttt ctggcttctg acagagaagg atttcaaata attgattaaa attctatttt
      301 ttgattataa acacattgaa attattgaag ggttcaaaag gaacattgaa ccgcccaaaa
      361 aatatggcgg aaaccccgtc tttgtttcag gtcatccact cgaagaaaac tggatttcac
      421 tgtatggttc tgttataaga aaacccgatg gatgctggca aatgtggtat accaccatga
      481 tgaacaacaa aaaaaatctt gtccttggtt atgcggaaag tgaagatgga atccactgga
      541 aaaggattga gaatgacgca gtaaagataa atgaaaccag gacgcatttt gtctttgaca
      601 gggaacccca tggagcagca attatttatg atgaacagga aacaagagaa aactggaaat
      661 ataaaatgat atgtggcgcc tcgctatctg gatgtatctc ggttttccat agcagagatg
      721 gcattcactg gattcctgca caaaaaaacc cagtaattgg aacaaatcca gattgtccta
      781 tgggatttct gaggttgccc tctgataagt atgtagcata tcacagacca gtttttggag
      841 acagaaggat agcaaggtct gaatcctggg attttataaa ctggtctgat gcaaaaaccg
      901 tgatcgacca gtctcctctt gacccgccgc aaacacagtt ttatggaatg ggagccgcat
      961 catacggaga atatgaaatc ggaacacttt ggatatatca cacagatcac aacgattttg
     1021 gtttctggaa aatggaagga taccaggaac ctgaattagt atataccaga accggatacg
     1081 cctggcacag aattgaaata aatatgccat ggattaagag aggggaaaaa ggggcctggg
     1141 attgtggaca gattcagcca gccagtgcac cagtatttct tgaagatgaa atacgtgttt
     1201 actatgcagg aacaagaaca acgcacggca aaagtttaga aacctggaat acatctgagc
     1261 cacgatgtgg catcgggttt gtttctataa aacctgatag atttgccggg ctggtagcct
     1321 ccgataaagg cattttgtta acaaggcctt tctggagtga agtacctcaa ttttttgtaa
     1381 acgcaaacat tgaaggattt ctctgcgtag aaattacaga tgtttctgga aaaccagtcc
     1441 cggggtttga acttgaaaat gcaataccca tgaaaggaga ttccctttat catccctgca
     1501 ggtggaaaaa taatacgggt acttcaggtc ttgtgaataa agatattaga tttaaggttg
     1561 tagcaaataa tgcgaaagtt tattctttga tggctggcag tgaaaaggac gttgcaaaat
     1621 accgggattt tcgtgttacc tattttccat ttgaaagaga aaaaaatttc atataggtgt
     1681 agaaaaattt tgaaaaacca ccaacaaatg gagggaaaat gaaaaaaatt ttaatcagta
     1741 tattattgtt ttctggaagt ttgttatttt cagacgtttc ttcctttaaa acacagttct
     1801 ttacaccggt tactgacctt gtcactcctc atataacctg gctttcccct tacaaaaatg
     1861 gaaaattaaa tcttttgtgg attacataca gaagggatgg tggtttcagg gaaattattg
     1921 aactcagtca gaggatggac attaatttca cagttttcac tctcgcaact cctgataaat
     1981 ttactccctg gtcgagttat tataaaccgg ttcctgtcac agatgaagaa taccagaaag
     2041 acttggaaga a
//
LOCUS       DAORYU010000257         2047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_36869_length_2047_cov_3.982430, whole genome shotgun sequence.
ACCESSION   DAORYU010000257 DAORYU010000000
VERSION     DAORYU010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36869_length_2047_cov_3.982430"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /gene="rsmH"
                     /locus_tag="PLW07_06985"
     CDS             complement(<1..890)
                     /gene="rsmH"
                     /locus_tag="PLW07_06985"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOL50311.1"
                     /translation="MERLLDKGAGIIRENSRNASWKLINHQPVMVGKVLFFLNVRPGC
                     VYIDATCGTGHHARAILEKAKCECRVLCVDKDHRAIERAKEYLREFLDRVIFVRDGFE
                     NIQEIIKRLNIDKISGILFDLGFSSEQISDPGMGLGFRQDALLDMRFDQSSPVSAYDV
                     VNKWPEKNLEEIFRLYGEMRDAKRIARLICKERKIKPFETTRHLADFIARYRRTRKSI
                     HPATQVFQAIRICVNNELENLKAGLEGSLKLLAPGARIVVISYHSLEDRIVKNFMRTK
                     EQLKVLTKKPVVPDEMEKSI"
     gene            complement(821..1258)
                     /gene="mraZ"
                     /locus_tag="PLW07_06990"
     CDS             complement(821..1258)
                     /gene="mraZ"
                     /locus_tag="PLW07_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007086260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOL50312.1"
                     /translation="MFIGEYWGKIDDKGRVVIPSKLRKDLFQHNIKNVYITRGLENCL
                     FVFPENIWSAQTERLKSLPFTKVDPRAFTRLFFSGAFQSKIDRQGRIVLPQHLASYAG
                     LKDNIVIIGVGDRLEIWSEDKWNDYSTKALESYGKIAETLLGS"
     gene            complement(1404..>2047)
                     /locus_tag="PLW07_06995"
     CDS             complement(1404..>2047)
                     /locus_tag="PLW07_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50313.1"
                     /translation="SLEQSYKKMPVDITILIEYKVFEPGTYSTTIPDWGTSFALAKGL
                     GSNVGVLVDLGHHHHGTNIEQIVMILISENMRAGFHFNTRYAADDDHSVEPNQQMARI
                     FYELVKGNVVVNTDPKKNWAYMIDQCGGRENRIQALLHSVDSLQLSLAKGMLVQTEKL
                     LEYQEKDDIILANRFFNDATINADVRPIVAKARLELGVPVCPVEAYIESGYQK"
BASE COUNT          581 a          457 c          343 g          666 t
ORIGIN      
        1 ttgatggatt tttccatctc atcaggaaca actggttttt tggtaagcac ttttagttgc
       61 tcttttgtcc tcataaaatt ttttacgatt ctatcttcaa gagaatgata cgaaatcaca
      121 acaatccttg cgcctggagc caggagtttc aatgaacctt caagtcctgc tttcaaattt
      181 tcaagctcgt tgttcacaca tattcgtatt gcctgaaaca cctgggttgc aggatgaata
      241 gattttcttg ttcttcgata tctggcaata aaatcagcaa gatgtcttgt cgtttcaaat
      301 ggttttatct ttctttcttt gcatatcaaa cgtgcaatcc tttttgcgtc cctcatttca
      361 ccatataagc gaaaaatttc ttcaagattt ttttctggcc atttgttgac aacgtcgtat
      421 gcgcttactg gcgacgattg gtcaaatctc atatcaagca gtgcatcctg acgaaagcca
      481 agccccatac cagggtctga tatctgttca gaagaaaagc caaggtcaaa taaaattcct
      541 gatattttgt caatgttcaa tcgcttgatg atttcttgta tattttcaaa accatctcta
      601 acaaaaatca ctctatcaag aaattctctg agatattcct tcgccctctc aatggcgcgg
      661 tggtccttat ccacgcataa aaccctgcat tcacattttg ccttctcaag tatggcgcgg
      721 gcatgatgac ctgttccaca tgttgcatca atatatacgc agccaggtct cacgttaaga
      781 aaaaacagca ccttcccaac catcacaggc tgatgattga tcagcttcca agaagcgttt
      841 ctgctatttt cccgtatgat tccagcgcct ttgtcgagta atcgttccat ttatcttcag
      901 accagatttc aagcctgtca ccaacaccaa taatcacgat attatccttt aaacctgcat
      961 aactggcaag atgctgcggc agcacaatac gaccctgcct gtcaattttt gattgaaaag
     1021 cgcctgaaaa aaataatctt gtgaaggcac ggggatcaac ttttgtaaaa ggaagtgatt
     1081 tcaacctttc ggtttgagcg ctccatatgt tttcagggaa aacaaaaaga caattttcaa
     1141 gtccacgagt aatataaaca ttttttatat tatgctgaaa aaggtctttc ctgagttttg
     1201 aaggaatcac tactctgcct ttgtcatcta ttttgcccca gtattctcca atgaacacca
     1261 cttcctccca ttttctacca ccaatatata aaaccatact gataaaattg tcaagttttt
     1321 tattcctggc agattaaatt gatcaaaaat tcaaagccag tcgatagaaa aattatgtat
     1381 taattcgagc gttccttttc aatttatttc tgatatccac tttctatgta cgcttcaacc
     1441 ggacatacag gcacaccgag ttcaagtcgt gcctttgcaa caatcggtct gacatcagca
     1501 ttaatcgttg cgtcattgaa aaatctgttg gcaagtatga tatcgtcttt ctcctgatat
     1561 tctaaaagtt tttctgtttg aacaagcatt ccttttgcaa gcgatagttg aagtgaatct
     1621 acagaatgca ataatgcctg gattctgttt tctcttcccc cacactggtc tatcatatat
     1681 gcccagtttt tcttcgggtc agtattgacg acaacattgc ctttcacaag ttcataaaaa
     1741 attcttgcca tctgctgatt cggttcaaca gaatggtcgt cgtcagcggc gtatctggta
     1801 ttaaaatgaa atccagcgcg catgttttca gatattaata tcatcactat ttgttcaata
     1861 tttgttccgt ggtgatgatg tccaagatcc accagtactc caacattcga accaagccct
     1921 ttcgcaagag caaatgatgt cccccaatca ggaatcgtgg tactatatgt tcctggctca
     1981 aaaaccttgt actcaataag aatggtaata tccactggca tttttttata agattgttcg
     2041 agagaat
//
LOCUS       DAORYU010000258         2037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_37141_length_2037_cov_5.009082, whole genome shotgun sequence.
ACCESSION   DAORYU010000258 DAORYU010000000
VERSION     DAORYU010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37141_length_2037_cov_5.009082"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1878)
                     /gene="bamA"
                     /locus_tag="PLW07_07000"
     CDS             complement(<1..1878)
                     /gene="bamA"
                     /locus_tag="PLW07_07000"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOL50314.1"
                     /translation="MSPRHLIRVLSLLISFLVPVFLYSDTNIVASITIKGNTTVSSTR
                     INLMLRTKPQGVFDEQLWKRDIQSLMDTGYFSSVKYEMKDTEKGLDITIFVEENPVVE
                     GIKFNGARIFKKTELEKMAEIKKGDYCNDALVKRAIAGLKQKYEEKGIYFTEIVATTQ
                     PAANNRVIVIFDIHEGKGKLYVGTINFSGNKAFDSLILRKKMKIKQRKMPFIRGTFKP
                     EILEDDIKRLEEFYHNNGYPECSIDRKTSVENKMIVIDIVVNEGEKFYFGKTEFSGNL
                     IFDTNKIKKLVEYHEGDVYSQMKFDKTLSNIRRIYSDSGYIRPIIRPVPEITDSKINF
                     SITIDAGTKVFINEIKIAGNTVTKDKVIRREMAIYPGDEFSGEKLRKSFNNLSDLQFF
                     DEIEIKPEPTEDEKLVDLDVIVKDKERTGMFTFGAGYSSLEQGVGFIGIQQRNFDISD
                     PPHFRGAGQNINLEAMMGGVTKHFVLSFTEPYLFDRPISFGPDIFITEKTWDDYTEKH
                     NGVDIRIGRRWKNFSIGCKLMTDEIKLSDIQIPEFQSQYGTNRVNSLTVTFGFQNLDR
                     RILPKDGDQIQLTCEYAPSFLGSDLEYWKATVENDYYKSLGRFVFHSKTYAGSVSHLG
                     ST"
     gene            complement(1835..>2037)
                     /locus_tag="PLW07_07005"
     CDS             complement(1835..>2037)
                     /locus_tag="PLW07_07005"
                     /inference="COORDINATES: protein motif:HMM:NF021915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50315.1"
                     /translation="QFGARPLRRAISRYLEDPLSEELLKENFPQHSHIVVKFDKNRLI
                     FTLTEGKEHVTATPDKSPVTPD"
BASE COUNT          575 a          420 c          314 g          728 t
ORIGIN      
        1 tgtagagccc agatgactta ctgaacccgc ataggttttg ctgtgaaaaa caaatcttcc
       61 taaacttttg taataatcgt tttcaactgt tgccttccag tattcaaggt cgctgccgag
      121 aaaagaagga gcatattcac aggttaactg aatctggtct ccatctttcg gcaatattct
      181 tctgtcaaga ttttgaaaac caaacgttac tgttagacta tttaccctgt tggttccgta
      241 ctgcgactga aattcaggta tctgaatatc actcagtttg atttcatcag tcatcaactt
      301 acatcctatt gaaaaattct tccatcttct gcctattctt atgtcaacac cattgtgttt
      361 ttcagtgtag tcatcccatg ttttttctgt aataaaaata tccggaccga aagaaatggg
      421 tctgtcaaaa agatacggct cagtaaaact caacacaaaa tgttttgtaa caccacccat
      481 cattgcttca aggtttatgt tctgtccggc tcccctgaaa tgaggtggat ctgaaatatc
      541 aaaattcctc tgttgaatgc ctatgaatcc gactccctgt tcaagagaac tgtaacccgc
      601 accaaacgta aacattccag ttctttcttt atcttttact attacatcaa gatccacgag
      661 tttttcatct tctgttggct ctggcttgat ttcaatttca tcaaaaaatt gaaggtctga
      721 tagattatta aaacttttcc ttaatttttc tccagaaaat tcatcccctg gatatattgc
      781 catttcacgt cttattacct tatcctttgt taccgtgttg ccagcaattt tgatttcatt
      841 aatgaaaacc tttgtgccag catcaatggt aatcgagaaa ttaatttttg aatctgttat
      901 ttctggaaca ggtctgatta tgggtcttat ataacctgaa tccgaataaa tgcgacgaat
      961 gttcgaaagt gttttgtcaa atttcatctg agaataaaca tctccctcgt ggtattcaac
     1021 aagttttttt attttgtttg tatcgaaaat gagatttcca gagaactctg ttttcccaaa
     1081 ataaaacttt tctccttcgt tcacgacaat atcaattaca atcattttat tttcaactga
     1141 cgtttttcta tctattgaac attcaggata tccgttgtta tggtaaaact cttcgagccg
     1201 ttttatatca tcctcaagaa tttcgggctt aaaggtccct cttatgaaag gcattttcct
     1261 ctgtttaatc ttcatcttct ttctgagaat caaagaatca aatgctttat tccctgaaaa
     1321 atttattgtc cccacgtata atttcccttt cccttcatga atatcaaaaa tcacaattac
     1381 tctgttattg gcagctggct gggttgtggc aacaatctca gtaaaatata tccccttttc
     1441 ttcatacttt tgtttcaatc ctgctattgc ccttttaaca agcgcatcat tgcaataatc
     1501 gccttttttt atttccgcca ttttctcaag ttctgttttc ttgaaaatcc tggctccatt
     1561 aaattttatt ccttcaacaa ctggattttc ttcaacaaaa atagtgatat caagtccttt
     1621 ttcagtatct ttcatttcgt attttactga agaaaagtat cctgtatcca tcaaggattg
     1681 aatatctctt ttccagagtt gttcgtcaaa cacaccctgc ggctttgttc tcaacatcaa
     1741 atttattctt gtggatgaaa ctgttgtatt tcctttgatg gtgatactgg caacaatgtt
     1801 ggtatccgaa taaagaaaaa caggaacgag aaaactaatc aggagtgaca ggactcttat
     1861 caggtgtcgc ggtgacatgt tctttccctt cagttaaagt gaaaattagt ctatttttat
     1921 caaactttac aacgatatgc gaatgctgag gaaaattctc tttcaaaagt tcttcggaca
     1981 atgggtcctc aagatacctg gagatggctc ttctaagagg tcttgctcca aattgag
//
LOCUS       DAORYU010000259         2012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_37779_length_2012_cov_3.600409, whole genome shotgun sequence.
ACCESSION   DAORYU010000259 DAORYU010000000
VERSION     DAORYU010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37779_length_2012_cov_3.600409"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1439
                     /locus_tag="PLW07_07010"
     CDS             <1..1439
                     /locus_tag="PLW07_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50316.1"
                     /translation="CTFIWDKNLPGRPRAADITYLTKNLPSFYNKGIRLISGWTEDSW
                     GPAGLGNYILARLLWEVNEAKNVNEIFNDFLEKSFGPAKQPMEKFYRLIYKIDEKDNR
                     PLFSDDLIGKMYRYLDEALSRTNDPSIKSRIYDLVLYTGYVEKYLRYVHSLPQEKQAC
                     FEEMVKHIYRMRNTQMIDLVAAYVTLQDMTKDVTVPESARWNVPEGKNPWKIKEPFTE
                     EEIKTMLKNGIASHSVFPVEPVFYSTNLVPVKNLKNIVIKDKELIGDEGVQAGASQTI
                     YTWVEKAPAKIKLQVKGGLISFYRDYGNVYIKLFSSKFPDRPVAEDRSVPPDGNPHEI
                     VMETSYTGLHWFYITTGGDRAKVKILEPDMAWTFESGITNRSFQPGTMWSLYFYVPKG
                     TKIVAGYSDGGGGKILDGNGKEVFSFEQLPRYSYFKVNVPEGQDGTLWMFSKCNGNKI
                     LLTVPPYLSVSKEKLLLPQEVVERDLLR"
     gene            1457..>2012
                     /locus_tag="PLW07_07015"
     CDS             1457..>2012
                     /locus_tag="PLW07_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50317.1"
                     /translation="MRVFLMLLCLAQACFAAEVCIEAEDFQIVSGWKVIKSFEGYFPA
                     RPATWSGNRIRDDEQVALAIAKKKVRIDEDGKYNLWVRYESAYGFNSLFNIEIVQNKK
                     IVFAGEFGNRDSFKYFPMERKWQIQGPWAYHNTDYVYEKASVDLKKGEATIMLKKGKD
                     VYPSCRRIIDFVFLTTDLSVEPGDD"
BASE COUNT          672 a          320 c          462 g          558 t
ORIGIN      
        1 tctgtacctt tatatgggac aaaaatttac ctggacgacc aagggcagca gacatcactt
       61 atttaacgaa aaatcttccg tctttttaca acaaaggtat ccggttaatc agtggctgga
      121 cagaagatag ttggggtcca gcagggcttg gtaattacat acttgcaaga ttattatggg
      181 aggtaaatga agcaaaaaat gtgaatgaaa tttttaacga ttttcttgaa aaatcatttg
      241 gtcctgcaaa acaaccaatg gagaagtttt accgcttaat ttacaaaatt gacgagaaag
      301 ataacagacc tttgttcagt gatgacctga taggaaaaat gtacagatat cttgatgagg
      361 cgctttctcg cacaaatgac ccatcgataa agagcagaat ttatgacctt gttctttaca
      421 caggatatgt ggagaaatat ctcagatatg ttcacagcct tccgcaggaa aaacaggcat
      481 gttttgaaga aatggtcaag catatttaca ggatgagaaa tacccagatg attgaccttg
      541 ttgcagcata tgtgacattg caggatatga cgaaggatgt aactgtgcct gaaagtgcaa
      601 gatggaatgt gcctgaagga aaaaacccct ggaagataaa agaaccgttt acagaggagg
      661 aaataaaaac aatgctgaaa aatggcattg cttcacattc ggtgtttcct gtggaaccag
      721 tgttttacag tacaaatctt gtgcctgtga aaaatttgaa gaatattgtc ataaaagata
      781 aagaactaat aggggatgaa ggcgttcagg caggtgcttc tcagacaatt tatacctggg
      841 ttgaaaaagc acccgcaaaa ataaaacttc aggtcaaagg tggtcttatt tctttctaca
      901 gggattatgg aaatgtttac ataaaacttt tttcttcaaa atttcctgac agacctgtcg
      961 cagaagatag aagcgttcct cctgatggta atccacatga aattgttatg gaaacttctt
     1021 atacaggact gcactggttt tatattacga ctggcggaga cagggcaaaa gtgaagatac
     1081 ttgaacctga tatggcatgg acctttgaat caggtattac taacagatca tttcaacccg
     1141 gaaccatgtg gtccctgtat ttttatgttc cgaaaggaac aaaaattgtg gctggatact
     1201 ctgacggtgg aggtggaaaa attcttgatg gcaatgggaa ggaagtgttt tcgtttgagc
     1261 aactgccgag atacagttat ttcaaggtaa atgtgccaga aggtcaggat ggaacgctct
     1321 ggatgttttc taaatgcaat ggaaacaaga ttttactcac agtaccaccg tatctttcag
     1381 tatcaaaaga aaaattactt ttgccacagg aagttgtgga acgagacctt ctacgataac
     1441 caacaggggg atgaacatga gagtattttt aatgttattg tgcctggctc aggcgtgttt
     1501 tgctgcagaa gtgtgcattg aagcagaaga ttttcagata gtttctggct ggaaggtgat
     1561 taaatctttt gaggggtatt ttcctgcaag accagcaacc tggagtggaa atagaatcag
     1621 ggacgacgaa caagttgcac tggcaattgc caaaaaaaag gtcagaattg atgaagatgg
     1681 aaagtacaat ctctgggtaa gatatgaatc agcatatggt ttcaatagtt tattcaatat
     1741 tgaaattgtg cagaataaaa aaatagtgtt tgctggagaa tttggaaaca gggacagttt
     1801 caaatatttc ccgatggaaa gaaagtggca gatacagggt ccgtgggcat atcataatac
     1861 tgattatgta tacgaaaagg catctgttga tttgaaaaaa ggcgaagcga caatcatgtt
     1921 aaaaaaaggc aaagacgttt atccatcatg ccgcaggata atagattttg tgtttcttac
     1981 cacagattta tctgttgaac caggtgatga tt
//
LOCUS       DAORYU010000260         1992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_38367_length_1992_cov_2.729479, whole genome shotgun sequence.
ACCESSION   DAORYU010000260 DAORYU010000000
VERSION     DAORYU010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38367_length_1992_cov_2.729479"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..1141)
                     /locus_tag="PLW07_07020"
     CDS             complement(98..1141)
                     /locus_tag="PLW07_07020"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HOL50318.1"
                     /translation="MENTITSVSSDLNRRHNYYTVLHVIRAHHRISITEISHLTRISY
                     PTVANIVQELKKKKLVRYRKINETRIGRKPCVVEFVPSHGSIIGLEIGCGLNGVITTL
                     NGKIITRKFRQKYDGCITLAEILSFIEKLIRNKQSDSHENLVGLGVAIAGYVNDKGEY
                     LVGRFDHSVWMPVKKVLEETFNVPVVVENDANAAMRAEYSFGVARGIQNAVYILKRGA
                     GIGMGLLLNGNIYRGTEGFAGENFELFDGKVLDMPGNKLVLHNDFLEGLYRIAGLLNP
                     EMVILGGELSDVSEHVLTEAEQNLRKYQDKNIKIALKTAKVGKDGAVLGAVDSVMELL
                     FGFPMKIKNFQKI"
BASE COUNT          631 a          425 c          320 g          616 t
ORIGIN      
        1 gttcaaataa aatagaggcg agtatcgcaa tgattgcgat taccacaaga agttctatga
       61 gagtaaatcc ttttcttttc atttctctca ccccctttca aattttttga aaatttttta
      121 ttttcattgg aaatccaaaa agcaactcca ttacagaatc aacagcgcca agaacagcac
      181 catcttttcc aacttttgct gttttgagag caatttttat atttttgtcc tgatatttcc
      241 tcaagttttg ttccgcttca gtcaaaacat gttcgcttac gtccgataat tcaccgccca
      301 aaatgaccat ttcaggatta agcaatccag caattctgta aagtccttca aggaaatcat
      361 tatgcagaac caatttattc cctggcatat ccagaacttt tccatcaaaa agttcaaaat
      421 tttcaccggc aaacccctct gttcctctgt aaatatttcc attcaaaagt agccccatcc
      481 cgatacccgc accccttttc aaaatataca ctgcgttctg aatacccctt gcaactccaa
      541 aactatactc cgcacgcatc gcggcattgg catcattttc gacaacaact ggaacgttga
      601 aagtttcttc taaaaccttc ttgacgggca tccatacaga atggtcaaac cttcctacga
      661 gatactctcc tttgtcattt acatagccgg ctatagcaac acccagaccg acaaggtttt
      721 cgtgactatc agattgtttg tttcttatca atttctcaat aaaagatagt atttctgcta
      781 aggttatgca tccatcatat ttttgtcgga atttccttgt gataattttt ccattcaagg
      841 tagtaatcac tccattgagt ccacagccaa tttccagacc gataatagaa ccatgagaag
      901 gtacaaactc aacaacacat ggttttctac ctattcttgt ctcattgatt tttctgtatc
      961 tgacaagttt tttctttttg agttcctgaa cgatatttgc cacagtggga taactaatcc
     1021 tggtgaggtg ggaaatttct gtaattgata ttctatgatg tgcgcgtata acgtgcaaaa
     1081 cagtatagta attgtgccgc ctgtttaaat ccgatgacac acttgttatc gtattttcca
     1141 ttatatcacc agtaatataa aaacatattc ttggaacttg tcaagttttt taagatattt
     1201 tcaaaaggct ttaaaaaaat ctgctctgca gatggaaacc gaaacattga atttagtcat
     1261 ctttttcagg tactgacagg ggttataggt aaatctaaaa acccgggaaa cttagaaaaa
     1321 atatagaaaa aatgctatct gtttttgaaa aaattaattc taaagccata atggcaggat
     1381 aatatgagta tgggattttt aatgtaatta aaccctgtag ggtatctaca ttcttttcca
     1441 gtggatgtca ccacaatctt aaaccatcca gaaaagcaaa gaaaaaaaac agtttcccca
     1501 gttccattct atgatgaatg accggaatgt acatcctgca tcgtggacga aaatggaaaa
     1561 ataaaaaccc ttagtgcggt ttcatccaga ggtatttaag aagatttgaa tataaaaaca
     1621 cctacctctg gggacatgga tagcgaagaa aaagtcctgt gaacaacgtt ctattgctta
     1681 gtaatatctc attcgcatta gtgaaaaaac atttcctgaa gttaagcatc tgagcaattc
     1741 aggggcagaa aagaacagcc agtccgttct tcacgatggg tacaatgtca cacagtatcc
     1801 gacagttctc gattaaaacc cagcagtcag cagggaagta cccaaatatt atcaaagctc
     1861 ctttgccctc ttcggccggg gcataccaaa cccattgcag gggtcaagat aaaattcaac
     1921 tttttttatt gcatccttta taattttctt cttaaatcta aaaggcctac catgtgcagg
     1981 acacacaatt gc
//
LOCUS       DAORYU010000261         1985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_38533_length_1985_cov_5.324870, whole genome shotgun sequence.
ACCESSION   DAORYU010000261 DAORYU010000000
VERSION     DAORYU010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38533_length_1985_cov_5.324870"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="PLW07_07025"
     CDS             <1..716
                     /locus_tag="PLW07_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HOL50319.1"
                     /translation="LGMKLENVDISMLGRAKRVIVDKENTTIVEGAGKKSDIEGRISQ
                     IKKEIEKSTSDYDKEKLQERLAKLAGGVAVVNVGAPTESDMKERKARVEDALSATRAA
                     VQEGIIPGGGVALLRCQREIEKVEYADEDERTGGKIVYKALEAPLRQIAENSGMDGAV
                     VVDKVRSADKESFGFNAEKDQFEDLVKAGIIDPTKVARTALENAVSVAALLLTTETLI
                     TEKPEKKEKAPAAPPYPEY"
     gene            726..1043
                     /locus_tag="PLW07_07030"
     CDS             726..1043
                     /locus_tag="PLW07_07030"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOL50320.1"
                     /translation="MGPRILAGSLFLFAMERWHYYISGEVQGVGFRWYATSIAKELGV
                     NGWVKNLRDGRVEIIAEAEPDILESFTEKMKKSYLGLNIHYINIEKEKATGEFKDFKI
                     LFF"
     gene            1046..1438
                     /gene="queF"
                     /locus_tag="PLW07_07035"
     CDS             1046..1438
                     /gene="queF"
                     /locus_tag="PLW07_07035"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HOL50321.1"
                     /translation="MTKQYQDVAKSGLDTKLPEIDWFKNFHKNYTITIVIPEYTSVCP
                     KTGNPDFGKITIQYQPGDRVIELKSLKEYIFAYRNLGIFYENAVNKILKDIVSAIKPV
                     WISVKGEFTPRGGIYSIIEASWPARKKS"
     gene            1530..>1985
                     /locus_tag="PLW07_07040"
     CDS             1530..>1985
                     /locus_tag="PLW07_07040"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOL50322.1"
                     /translation="MENSRDKLIEAAINLGESIGLNRAVCQIYALLYLSEKPLSPTEI
                     GRILEMSKGNVSINLKTLEQWNAVRKVWRKGYSRALYAANENIEEIILNKLKTGLEKR
                     ISYARPLINEAKGKEKSKDDNFLKKLDRIEKLISKIEFFLDNFETIKSIT"
BASE COUNT          717 a          345 c          428 g          495 t
ORIGIN      
        1 aactcggaat gaaacttgaa aatgttgata tcagcatgct cggtcgcgcc aagagggtga
       61 ttgttgacaa ggaaaataca acaatcgtag aaggcgctgg caagaagtct gatattgaag
      121 gaaggatcag ccagataaag aaagaaattg aaaaatcaac atcagattat gacaaagaaa
      181 aactgcagga acgtcttgcg aaacttgctg gtggtgttgc tgtggtgaat gttggagctc
      241 ctacagaatc agacatgaag gagaggaaag cccgtgttga agacgctttg agtgcaacga
      301 gagcagcagt tcaggaaggt atcatacctg gtggtggtgt tgctcttctt cgctgccaga
      361 gagaaattga aaaagtggaa tatgccgatg aagatgaaag aactggcgga aagattgtgt
      421 acaaagccct cgaagcacct ctgagacaga ttgcggaaaa ctctggcatg gacggagcag
      481 ttgtcgttga caaggttcgg tctgctgata aggaaagttt cggtttcaat gcagaaaaag
      541 accagtttga agaccttgtc aaggcaggta tcattgaccc aacaaaggta gcaaggactg
      601 ctcttgaaaa cgctgtcagt gttgctgcgc ttcttcttac tacagaaact ctgattacag
      661 aaaaaccaga gaaaaaagag aaagcacctg cagcaccgcc gtatcctgaa tattgataga
      721 taacaatggg accccgtata cttgcggggt cccttttcct ttttgcaatg gaacgatggc
      781 actattacat ttctggagaa gtgcagggag tgggattccg atggtatgca acaagtattg
      841 caaaagaact cggggtcaac ggctgggtga aaaaccttcg ggatggaagg gttgaaatta
      901 ttgcagaagc agaacctgac attctggaat cttttacaga aaaaatgaaa aaaagttatc
      961 tgggtttgaa tattcattac atcaatatag aaaaagaaaa ggcaaccggt gaattcaaag
     1021 atttcaaaat tctgtttttc tgacgatgac aaaacaatat caagacgttg ctaaatcagg
     1081 acttgataca aaacttcctg aaattgactg gtttaaaaac ttccacaaaa actatacaat
     1141 caccattgta attccggaat acacttctgt atgtccaaaa acaggaaatc ctgattttgg
     1201 gaaaattacc attcaatatc agccaggaga tagagtcatt gaactgaaat cattaaagga
     1261 atacatcttt gcttacagga atctcggaat attttacgaa aacgctgtga acaaaatttt
     1321 aaaagatatt gtttcagcaa tcaaacctgt ctggatttca gtgaaaggtg aatttacgcc
     1381 acgaggtggc atatactcca ttattgaagc atcctggcct gcaagaaaaa aatcctgaac
     1441 aattcattct tattatcagt ttcccctggc atcctcttga caaaattgtt aaaatgattt
     1501 aaaacattct aaacaaagga taaaaaccag tggaaaactc acgagacaaa ttaattgaag
     1561 cagccataaa tttaggcgaa agtataggat tgaatagagc agtgtgccag atttacgcgc
     1621 ttttgtacct ttccgaaaaa cctttatctc ctacggaaat tggcagaatt cttgaaatga
     1681 gcaaaggcaa tgtcagtata aatctcaaaa cacttgagca atggaacgca gtgagaaagg
     1741 tctggagaaa aggttattcc agggcccttt atgcagcaaa tgaaaacata gaagaaatta
     1801 ttctcaataa attgaaaact ggccttgaaa aaagaatttc atatgccaga ccactgatca
     1861 atgaggcgaa aggaaaagaa aaatcaaaag atgataactt tttaaaaaaa ctcgatcgaa
     1921 ttgaaaaact tatttcaaaa attgaatttt tcctggacaa ctttgaaaca atcaaatcaa
     1981 taaca
//
LOCUS       DAORYU010000262         1983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_38585_length_1983_cov_4.164938, whole genome shotgun sequence.
ACCESSION   DAORYU010000262 DAORYU010000000
VERSION     DAORYU010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38585_length_1983_cov_4.164938"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="PLW07_07045"
     CDS             complement(<1..269)
                     /locus_tag="PLW07_07045"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HOL50323.1"
                     /translation="MAENKNQNKKKNIKPFVPLRDIVVFPNMVVPLLVGRQRSINAVE
                     EAMSTDNMIVLAFQRDPKVEGPRLDDIYQIGVNANVLVFVPQSISP"
     gene            complement(272..886)
                     /locus_tag="PLW07_07050"
     CDS             complement(272..886)
                     /locus_tag="PLW07_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014451897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOL50324.1"
                     /translation="MNTKNRSYNQLIPYVIEVTERGERAYDIYSRLLEDRIIFIGFPI
                     TDTVANAVIAQLLFLQNKDASKDVSLFLNTPGGSITAGLAIYDTIQFVKCDVATYCIG
                     QAASMGAVLLAGGKKNKRFVLPHSRVLIHQPFGEIGGTTTDVSIHTKEMIRLRSIINQ
                     VLAKHTGQTLKKIEKDTERDYFMDAEEAVKYGLADKIIYPNNKS"
     gene            complement(861..1877)
                     /locus_tag="PLW07_07055"
     CDS             complement(861..1877)
                     /locus_tag="PLW07_07055"
                     /inference="COORDINATES: protein motif:HMM:NF017509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOL50325.1"
                     /translation="MKVEIQQLPKYGRKMLFEIDAEQVKEKKTEIIQEIQKTAEVPGF
                     RKGKVPEQVIESRYSDVIRKNVLERIVTDSYLAAVKEHNLSPVIDPEVADVKFEDTIS
                     FSVYVEVKPEVSVNKYKELVVKEISPEPVTEQMVDEVLADWEKRKEFASSIIDPEKRA
                     AWRKKIREQLEQLSQHRAKTQEEQQLWEQLFKNSSIEIPEKLMLQRARYLTREQFHYI
                     DTQNKTKEEIEKIAEDLFEKMKPVAEEQLKKYFILEKISEMENIQVTDEEVEQAIKRI
                     ALTSGENYDQVKKRMMDSGKIYDLKNDIKIEKTFDLVKNTAQNIKKIILPGEEKHEHK
                     KQKL"
BASE COUNT          486 a          425 c          340 g          732 t
ORIGIN      
        1 ggacttatgc tctgcgggac gaaaaccaat acgttggcgt ttactccgat ttgatagatg
       61 tcatcaagac gcggtccttc aacctttgga tctcgttgaa aagccagcac aatcatgtta
      121 tctgtgctca ttgcttcttc aaccgcattg atggagcgct gtctgccaac aagaagcggt
      181 acgaccatgt ttggaaaaac aacaatgtcc cgcagtggca caaagggttt tatattcttt
      241 ttcttgtttt gatttttgtt ttctgccatg tttaactctt attgttcggg taaattattt
      301 tgtctgcaag tccatatttc actgcttctt ctgcatccat aaaataatct ctttcagtat
      361 ctttttcaat ttttttcagt gtctgtcctg tatgttttgc aagtacctgg ttaataatac
      421 ttctcaaccg tatcatttct tttgtatgaa tgctgacatc tgttgttgtt ccgcctattt
      481 ctccaaatgg ttgatggata agaacccttg aatgcggaag aacaaatctc ttatttttct
      541 ttccaccagc aagaagcaca gcgcccatac ttgctgcctg ccctatacaa tatgttgcaa
      601 catcgcactt tacaaattga atcgtatcat aaatggcaag cccagcagtg attgaaccgc
      661 caggggtatt caaaaacaaa ctgacatcct tggaagcatc tttattctga agaaaaagta
      721 attgcgcaat tacagcattg gcaacagtat cagtgatggg aaagccaata aaaatgattc
      781 tatcttcaag cagtcgtgaa taaatgtcat aggcgcgttc gcctctttca gtaacctcta
      841 tcacataagg aatgagctga ttataacttc tgttttttgt gttcatgttt ctcttctccg
      901 ggaagtatga ttttcttaat gttttgggca gtatttttga ccaggtcaaa tgttttttct
      961 atctttatgt catttttcag gtcataaatc ttcccgctgt ccatcattcg ttttttaacc
     1021 tgatcgtaat tttctccact ggtcagtgct attcttttga ttgcctgttc aacttcttcg
     1081 tctgtgacct ggatattttc catctctgaa attttttcaa gtatgaaata ttttttcaac
     1141 tgttcttcag caacgggttt cattttttca aacaggtctt ctgctatttt ttctatttct
     1201 tcctttgttt tattctgcgt gtctatgtaa tgaaattgtt ctctcgtaag atatcttgcc
     1261 ctttgtagca taagtttttc agggatttca atactactgt ttttgaaaag ttgctcccac
     1321 aactgttgtt cttcctgtgt ttttgctcta tgctgactta attgttcaag ttgttccctt
     1381 attttctttc tccatgcagc acgtttttca gggtcgatga tagaactggc aaattctttt
     1441 cttttttccc agtcagccag tacctcgtca accatttgtt ctgtgactgg ttctggcgag
     1501 atttccttca ccaccagttc cttatattta ttgactgaaa cctctggttt tacttctaca
     1561 tacacgctga aactgattgt atcttcaaat ttcacatcag ccacttcagg atcaatcact
     1621 ggagaaagat tgtgttcttt cacagcagca agatacgagt ccgtaacaat tcgttcaaga
     1681 acatttttcc gtatcacatc gctgtaccgc gattcaatca cctgttcagg cactttccct
     1741 ttacgaaaac caggaacctc tgctgtcttt tgaatttcct gaattatttc tgttttcttt
     1801 tccttaacct gttctgcgtc aatttcaaac aacatctttc tgccgtactt tggaagttgt
     1861 tgaatctcta ctttcatttt agatgctccc tggtaaaaaa gattgcaaaa ccgaccttga
     1921 aataaaattt aagagcgggg tacgggaatc gaacccgtat aatcagcttg gaaggctgat
     1981 gtt
//
LOCUS       DAORYU010000263         1969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_38953_length_1969_cov_6.248171, whole genome shotgun sequence.
ACCESSION   DAORYU010000263 DAORYU010000000
VERSION     DAORYU010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38953_length_1969_cov_6.248171"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="PLW07_07060"
     CDS             complement(<1..400)
                     /locus_tag="PLW07_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50326.1"
                     /translation="MRIEKQGFIFEAEKRSSTKKVCTFTSLFRHSSGKIFSSFRLGTR
                     KDSTDGNGVILASSLSTDWTMVFSNFKNEFNGHRGDIKVVELFERPDGNISAFLSWFD
                     CSKSKKLYDASSDTIQDSKLILVDSFDMGKT"
     gene            complement(408..1388)
                     /locus_tag="PLW07_07065"
     CDS             complement(408..1388)
                     /locus_tag="PLW07_07065"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HOL50327.1"
                     /translation="MQERKQVFITRMIPEPAIELLKKTCNVEVNMEDRVLTKHELISK
                     VKGKDAVLCLLTDTIDDEVLQAADRCKIFANYAVGYNNIDVESATRRKIIITNTPGVL
                     TDATSDLAWALLFAVARRIVEADAFTRQGKFKGWAPQLFLGQDITGKTLGIIGAGRIG
                     NNFARKSKGFSMRVLYYDVKRNFELEKDIGATFVDLDTLLKESDFVSIHVPLVPDTRH
                     LIGARELSLMKKTAILINTSRGPVVDEKALVDALKNRKIWGAGLDVYENEPDIEPELL
                     NLSNVVLLPHIASATFETRTKMAIMAAENILAVLRGEIPPNIVNPEVIRK"
     gene            complement(1397..>1969)
                     /locus_tag="PLW07_07070"
     CDS             complement(1397..>1969)
                     /locus_tag="PLW07_07070"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOL50328.1"
                     /translation="ISFLSGTYQEQNKHRAFDHLVESTEEICYYAKEKNSMIVELEIF
                     DYDIDKKSLIGPAKLAGNFARKIREKFDNFGLMIDLSHLPLLRESPAEAIVPIKDFIT
                     HIHIGNCFMSDKNSPLYGDQHVPFGTRGSENDVEQLAEFLQVLMDTGFFDKKQRPIVS
                     FEVKPLKGQDPEIVIANSKRTLNQAWLLIE"
BASE COUNT          550 a          432 c          325 g          662 t
ORIGIN      
        1 aggtctttcc catatcaaat gaatcaacca gaatcaattt tgagtcctgt attgtgtcac
       61 tcgaagcatc atacaatttt ttactctttg aacagtcaaa ccaggaaaga aaagcggata
      121 tatttccatc aggcctttca aaaagttcaa caaccttaat atctcccctg tgaccgttaa
      181 attcgttttt gaaattcgaa aaaaccatag tccagtcagt acttagtgaa gatgcaagaa
      241 tcactccatt tccgtcagtc gaatccttcc tcgtaccaag tcgaaaactt gaaaagattt
      301 ttccgcttga atgtctgaat aagctggtaa aagtacagac cttttttgta cttgaccgct
      361 tttctgcctc aaaaataaaa ccctgctttt ctatacgcat agcgctccta ttttcttata
      421 acctcaggat tcacaatatt tggtggtatt tctcctctta atacagcaag aatattttca
      481 gcagccatta tcgccatctt tgtccttgtt tcaaatgtcg cgcttgctat gtgaggcagt
      541 agtaccacat ttgaaagatt taatagttca ggttctatat caggttcatt ttcgtataca
      601 tcaagtcctg cgccccagat ttttctattc ttcagagcat ccacaagtgc cttttcatca
      661 accacaggtc cccgggaggt attgatgagt atggcagttt ttttcatcaa ggatagttct
      721 cttgccccaa tcagatgtcg tgtatctggg acaagcggca catgaattga aacaaaatca
      781 gattccttca gcagtgtatc aaggtcaaca aaggttgcac cgatgtcctt ttcaagttca
      841 aaatttcttt tgacatcgta atataatact ctcattgaaa agccctttga ctttctcgca
      901 aagttatttc ctattctgcc agcacctatg atacccagtg tttttcctgt aatgtcctga
      961 ccaagaaata attgaggtgc ccagccctta aatttgccct gccttgtaaa tgcatcggct
     1021 tcaacaattc ttcttgccac agcaaacaaa agtgcccatg cgagatcact tgtcgcatca
     1081 gtgagcaccc ctggcgtatt ggtaatgata attttcctac gggttgcaga ttccacatca
     1141 atgttgttgt acccaacagc gtaattagca aaaattttac acctatctgc tgcttgaagt
     1201 acctcatcgt ctatggtgtc agtgagaaga cagagtactg catcttttcc ttttactttg
     1261 cttataagtt catgcttcgt aagcactctg tcttccatat ttacttcaac attacacgtt
     1321 tttttgagaa gttcaatcgc tggctctggt atcatccttg tgataaacac ctgttttctt
     1381 tcttgcatgt tcactcctat tcaataagta accatgcctg gttcagtgtt ctctttgaat
     1441 ttgcaataac aatctctggg tcctgcccct ttaagggttt tacttcaaaa ctcactattg
     1501 gcctttgttt tttatcaaaa aatcctgtgt ccataagtac ctgtaagaac tcagcaagtt
     1561 gttcaacatc attttcacta ccacgcgttc caaatggaac atgctggtct ccgtaaaggg
     1621 gggagttttt gtcagacata aaacaattcc caatatgaat gtgagtaatg aaatctttaa
     1681 ttggtacaat tgcttctgca ggagattccc ttaataatgg aaggtgactg agatcaatca
     1741 tcaaaccaaa gttatcaaat ttttctctta tttttctcgc aaaatttcct gccaattttg
     1801 ctggacctat taaacttttt ttatctatgt catagtcaaa aatttcaagt tctacaatca
     1861 tggaattttt ctcttttgcg taatagcaaa tttcctcagt ggattcaaca agatggtcaa
     1921 aagcgcggtg tttattttgc tcttgatagg ttccacttaa aaaggaaat
//
LOCUS       DAORYU010000264         1958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_39243_length_1958_cov_4.233316, whole genome shotgun sequence.
ACCESSION   DAORYU010000264 DAORYU010000000
VERSION     DAORYU010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39243_length_1958_cov_4.233316"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="PLW07_07075"
     CDS             complement(<1..291)
                     /locus_tag="PLW07_07075"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOL50329.1"
                     /translation="MNWHILVNLYISGILIGSSVCMLSCGSVFLPFIFERNTDIKKTL
                     RIFVFFHAGKLVSYTLAGGLVGYSSGYVSALKNNRVFLFLGALFFLILGVLNL"
     gene            complement(288..1769)
                     /gene="amrB"
                     /locus_tag="PLW07_07080"
     CDS             complement(288..1769)
                     /gene="amrB"
                     /locus_tag="PLW07_07080"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOL50330.1"
                     /translation="MWNKNSWGLAVVVFCCFVFFSAEQIFSDTVRKPFFAGSFYPADG
                     KQLKSQVMSFLKAKSISEMPAGIIVPHAGYIYSGKTAGFAYSVIKDKKIDTVFLIGRS
                     HRARFLGIITDDRNIWESPLGDVRVDTQLVSEIVVKPGFRVDYRILDAEHGLEVQIPF
                     LQCAIKGEFKIVPILVGCENESELDIYANYLLPFVRKQKNSIIVMSTDLSHYYSLDVA
                     KQKDSSFISLLESGKFSLLIDEMQKGNAEACGGSAVYLGLKILSKISQIKVKLLDYST
                     SYNTTKDSSNVVGYAAMAVLFEHKKQSEDKTGGNMLNRQQRETLLKLARQTIEYHLSG
                     KKLPQINITDPVLCEKRGVFVTLRRKGQLRGCIGMILPRERLVDAVREMAIESATGDP
                     RFPPVTLNEMKDIEIEISVLTVPARVSSPDEIVLGRDGVIVKKGFRQGVFLPQVADET
                     GWTKEQFLSALCEHKAGLEPDAWKKPDTELYTFQAEVFSEKEF"
     gene            complement(1736..>1958)
                     /locus_tag="PLW07_07085"
     CDS             complement(1736..>1958)
                     /locus_tag="PLW07_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOL50331.1"
                     /translation="TPYKTLEDAVNIAKSTGLKHVYIGNVYDNPYENTVCSNCGKIVI
                     KRKGFEVLENHIRKGKCEYCGTKIPGVWQ"
BASE COUNT          599 a          442 c          302 g          615 t
ORIGIN      
        1 aagatttaat acccccagta ttaaaaaaaa taatgcgcct aaaaaaagaa atactcgatt
       61 attttttagt gctgaaacat agccagatga ataccctaca agtccgcctg ccagggtata
      121 agaaacaagt tttccagcat gaaaaaacac aaatatcctc aatgtttttt taatgtcagt
      181 attcctttca aaaataaaag gaaggaaaac gcttccacag gaaagcatac aaacactgct
      241 tccaattaat atcccgctga tatacagatt aacaaggatg tgccagttca aaattccttc
      301 tccgaaaaaa cttctgcctg aaatgtgtac aattcggtat ctggtttttt ccaggcatct
      361 ggttcaagtc ctgccttatg ctcgcacaac gctgaaagaa actgttcttt tgtccagccc
      421 gtctcatcag caacctgtgg aagaaaaaca ccctgcctga atcctttttt cacgatcact
      481 ccgtccctgc caagcacaat ttcatcggga ctggaaaccc tggcaggaac tgtcaggaca
      541 gaaatttcta tttctatatc tttcatttca tttaaggtaa ctggtggaaa tcttgggtcc
      601 cctgtcgcag attcaattgc catttctctc actgcatcta caagtcgttc cctcggcaaa
      661 atcataccaa tacaaccgcg caactgccct tttcttctga gcgtgacaaa cacccctctt
      721 ttttcgcaaa gcacagggtc tgtaatgttt atttgtggaa gctttttgcc actcagatga
      781 tattcaattg tctgccttgc aagttttaac aaagtttccc tctgttgcct gtttaacatg
      841 tttcctcctg ttttgtcttc cgattgtttc ttgtgttcaa ataatactgc cattgccgca
      901 tatccaacca cattactgct gtctttggtc gtattatatg aagttgaata atcaagtaat
      961 ttcaccttaa tctgagaaat ttttgataga atttttaatc caagatacac agcactgcca
     1021 ccacatgcct ctgcatttcc tttttgcatt tcatcaatta acaatgaaaa tttgccggat
     1081 tcaagaagtg atatgaacga actatctttt tgttttgcaa catccagtga ataataatga
     1141 gaaagatcag tactcattac gatgattgag tttttctgtt ttctcacaaa aggtaaaagg
     1201 tagtttgcat aaatatccag ttcactttca ttctcacagc caacaagaat tggaacaatt
     1261 ttgaattcac ctttgattgc gcattgaaga aatggtatct gaacctcaag accgtgttct
     1321 gcatcaagaa ttctatagtc aacacgaaat cctggtttaa caacgatttc agacaccagt
     1381 tgagtatcaa ccctcacgtc tccaagcgga ctttcccaga tatttctatc atcagtgata
     1441 attcccagaa acctggctcg atgacttctg ccaattagaa acacagtatc tattttttta
     1501 tctttaatga ctgaataagc aaatcccgct gttttccctg aatagatata gccagcatgc
     1561 ggaacaataa taccagcagg catttcactg attgatttcg ctttcaaaaa tgacataacc
     1621 tgtgacttca gttgttttcc atctgcagga taaaaagacc ctgcaaaaaa gggctttctt
     1681 acagtgtcag aaaaaatctg ctctgcacta aaaaaaacaa aacaacagaa aaccactact
     1741 gccagacccc aggaattttt gttccacaat actcgcattt tccctttctg atatgatttt
     1801 caagaacttc aaatcctttt cttttaataa cgattttacc acagttgcta caaactgtat
     1861 tttcataagg attgtcgtaa acattaccaa tatacacatg tttcagacct gtacttttcg
     1921 caatgttcac agcatcttcc agtgttttat atggagtt
//
LOCUS       DAORYU010000265         1952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_39411_length_1952_cov_2.917238, whole genome shotgun sequence.
ACCESSION   DAORYU010000265 DAORYU010000000
VERSION     DAORYU010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39411_length_1952_cov_2.917238"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="PLW07_07090"
     CDS             <1..525
                     /locus_tag="PLW07_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50332.1"
                     /translation="EPEADGMDYEKIRRIRGEFFTNFLRDSRKMAGKFNKKISIHFEP
                     GIEVTPEFDTRMQIHWDWQTWIREGLMDEIHLKYWSSQNTWIHSNVLPAARKMGISVY
                     IEDQATDPRSDIRAIELIENIIRETKNAGFDGYIFYETWTYVMLNRKGIPMPRGNAEG
                     IIRTAFGMVQKELK"
     gene            547..1872
                     /locus_tag="PLW07_07095"
     CDS             547..1872
                     /locus_tag="PLW07_07095"
                     /inference="COORDINATES: protein motif:HMM:NF023401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50333.1"
                     /translation="MEYKIAMIGGGSPGFSYFIAEALVNSEIFSDSCFTLMDINEERL
                     NASVGKIKNLVKEEKSGLKVVSTTNRKKALEGANFVITCCEINRIPFWIQDIEIPEKY
                     GIYQLQGENGGPAGQIHAMRNVTLFMNICKDIRKICPDAWLMNFTNPMSFLCTYFHRY
                     GNVKFLGFCHQVHGSFGVIAEMLGMEPGDLTMITVGINHFNWLIDVRRKGSCESYLNE
                     FLKRVRKSIYWKKNFKNIPHQKFTLELLNIFGAYPVGYDDHIVEYIPFFYEKKEWKKM
                     NYHSVKYELRKFLKSQVNRGIIGELKTEKPPFPKNLNHPYYKESPVEVAEALIANKTM
                     YLDAINIPNKGCVRNLPSDAIVDIPAVVFGGEARGIYIGELPSVCAELCRRQITIHNL
                     VVDATVKGDRTLALQAMCLDPYIRSVTQARNILNEYLRVYRKYLPQFFR"
BASE COUNT          680 a          282 c          423 g          567 t
ORIGIN      
        1 gaacctgagg ccgatggaat ggattatgaa aaaattagaa gaattcgcgg agaatttttt
       61 actaactttt tgagggactc aagaaaaatg gcgggtaaat ttaataaaaa aatttcaata
      121 cactttgaac caggtattga agtgacacct gaatttgaca ccaggatgca aatacattgg
      181 gactggcaaa catggataag agaaggacta atggatgaaa tacacctgaa atactggtca
      241 tcccagaata cttggattca tagcaatgtt ttgccagcag caagaaaaat gggaatttct
      301 gtgtacattg aggaccaggc aacagacccc aggtcagaca taagggctat agaattgata
      361 gaaaatatta taagggaaac aaaaaatgct ggattcgacg gatatatttt ctatgaaaca
      421 tggacatatg ttatgttaaa cagaaaagga attcctatgc cgaggggtaa cgctgaagga
      481 atcattagaa cagcatttgg gatggttcaa aaagagttga agtagataat tcgaagcgga
      541 gacagtatgg aatataaaat tgctatgatt gggggtggtt ctccagggtt ttcttacttc
      601 atagcagagg cacttgttaa ttcggaaatt ttctctgatt catgttttac actaatggac
      661 attaatgagg aaagattaaa tgcttctgta ggaaaaatta aaaatctggt gaaagaagag
      721 aaatcaggac tgaaagtggt atctaccaca aaccggaaga aggcactgga gggagctaat
      781 tttgtaataa cttgttgcga aattaaccgt attcctttct ggatacagga cattgaaata
      841 ccggagaaat acggtattta tcaattgcaa ggtgaaaatg gtggacctgc aggacagata
      901 cacgctatga gaaatgtcac tctttttatg aatatatgca aggatataag aaaaatttgt
      961 cctgatgctt ggctgatgaa cttcaccaat cctatgagtt ttctatgcac atatttccat
     1021 cgttacggga atgttaaatt tcttggattt tgtcatcagg ttcacggtag cttcggtgta
     1081 attgctgaaa tgctcggtat ggagcccggg gatttgacga tgatcacagt ggggataaac
     1141 cactttaact ggctcataga tgtaagaaga aaaggaagtt gcgaaagtta tcttaatgaa
     1201 tttttaaaaa gagtgagaaa gagtatttac tggaaaaaga attttaaaaa catacctcat
     1261 cagaaattta ctctggaact acttaatata tttggtgctt atcctgttgg ctatgatgac
     1321 catatagttg aatatattcc ctttttttat gaaaaaaaag aatggaagaa aatgaattat
     1381 cattctgtaa aatatgaatt gagaaaattt ctaaaatctc aagtgaacag aggtatcata
     1441 ggagaattaa aaaccgaaaa acctcctttt cctaaaaatt taaaccatcc ttactataaa
     1501 gaaagcccag ttgaagtggc tgaagcactt attgcaaata aaacaatgta ccttgatgct
     1561 atcaatatac caaacaaggg ttgtgttcgg aatttaccct ctgacgcaat agttgatatc
     1621 cctgctgttg tgtttggtgg ggaagccaga ggaatttata ttggagaatt accatcggtt
     1681 tgtgcagagc tgtgcagaag acagataaca attcataatc ttgttgttga tgccacggtg
     1741 aagggagata gaactctggc attgcaggca atgtgtttag atccatatat cagaagcgtc
     1801 actcaggcaa gaaatatttt gaatgaatac ttgagagttt atagaaaata tttgccacag
     1861 ttttttagat gaaaaaagga gtcatgaaat gaagagacat tcttgtttag gtttattatt
     1921 aatatttttt tctctgtctg tccacgctca gg
//
LOCUS       DAORYU010000266         1948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_39504_length_1948_cov_5.329107, whole genome shotgun sequence.
ACCESSION   DAORYU010000266 DAORYU010000000
VERSION     DAORYU010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39504_length_1948_cov_5.329107"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1153)
                     /locus_tag="PLW07_07100"
     CDS             complement(<1..1153)
                     /locus_tag="PLW07_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50334.1"
                     /translation="MKPAKNKNLFNVDCNGFFYKYNKKSLWYYPENRRFNAKHIHHCI
                     DILAESGIDTLVVNSNAQVAWYPSKVVETNLDGYKRGNKSFFFGHLLGQPMTRQQIEK
                     YLHEMVTLMDRYLDLAEDGVDWLEEATKACRRNKINPWVSIRMNDMHGASLMPEFDFM
                     NCKLFKNPQMRLKGITSNPEEPVQISWQGLNYEKVEVRNYMKALIQDCIENYDFEGIE
                     LDWTRWPLCCEVPAGEEAINTITQWHADIRKICEKKAKETKRPFYMGIRYAGTFDQLR
                     QIGIDIPEMARQKLIDFVCPTNTWQTSWDTPCDKISQQLDDDIAVYGVIELGTNWLMV
                     YFLGASANTHIGASDSRGYRLSAFSPEILRGNAAGKLAVGCDGIETFNFF"
     gene            complement(1163..>1948)
                     /locus_tag="PLW07_07105"
     CDS             complement(1163..>1948)
                     /locus_tag="PLW07_07105"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOL50335.1"
                     /translation="DYRNSIPCPFKSVDEVLNFDAAQEYGIPDIDERAMYFKQTLESA
                     QNEYPDVLVPGGYYKTIVSGCIASFGWEMFLEAVGTDPDRFGDYVIEGFFQVSLANFK
                     AWAKAKPFAFICHDDMVWSNGAIFNPAWYRKYVFPRYKKLWDVLKSEGIKVLFCSDGN
                     FTEFVDDIANAGADGFIFEPLTDLETIVKKYGDTHVIVGNADCRILTYGTKQDIEKEV
                     LRCMNTAKKCPGFIMAMGNHIPANVPVDNAMVYFEAVQKYGQR"
BASE COUNT          568 a          444 c          306 g          630 t
ORIGIN      
        1 agaaaaagtt aaatgtctct attccatcac agccaacagc aagttttcct gctgcattgc
       61 ctctcagtat ttcaggactg aaagcagaaa gtctgtaccc acggctgtct gaggcaccaa
      121 tatgggtgtt tgcgctcgca ccaagaaaat aaaccatcag ccagtttgtt ccaagttcaa
      181 ttactccata cactgcaata tcatcgtcaa gttgctggct tattttatcg cagggtgtat
      241 cccagcttgt ctgccaggta tttgttggac aaacaaaatc aataagtttc tgtcttgcca
      301 tttcaggtat atcaattcca atctgcctca actggtcaaa tgttccagca tacctgattc
      361 ccatataaaa tggtcttttt gtttcttttg cctttttctc acagattttt ctgatatcag
      421 catgccattg agtaatcgtg tttatcgctt cttcaccagc aggaacttca cagcataaag
      481 gccatcttgt ccagtcgagt tcaattccct caaaatcata gttttcaata caatcctgaa
      541 tcaatgcctt catataatta cgaacctcaa ccttttcata gtttaaaccc tgccatgaaa
      601 tctgtactgg ttcttcggga ttacttgtga tgcctttcag tcgcatctgt gggtttttga
      661 acaatttaca attcataaaa tcaaactctg gcatcaaact tgctccatgc atatcattca
      721 ttctgattga tacccatgga ttgattttgt ttcttctgca tgcttttgtt gcttcttcaa
      781 gccagtcaac accatcttct gcaaggtcaa ggtatctatc catcagtgtc accatttcat
      841 gaagatactt ttcaatctgc tgtcttgtca tgggttggcc taaaagatga ccgaagaaaa
      901 aacttttatt tcctctttta tatccatcaa gatttgtctc aaccacttta cttggatacc
      961 aggcaacctg agcattgcta tttaccacaa gtgtatcaat gccgctttca gcaagaatat
     1021 caatgcagtg atgaatatgt tttgcattaa aacggcgatt ttctggataa taccataaac
     1081 tttttttgtt atatttgtaa aaaaatccat tacaatcaac attgaaaagg ttcttattct
     1141 ttgctggctt cattggatat gcttatctct ggccatattt ttgcactgct tcaaagtaaa
     1201 ccatcgcatt atcaacagga acatttgccg gaatatgatt tcccatagcc ataataaatc
     1261 caggacattt ctttgctgta ttcatgcagc gtaacacttc tttttcaata tcctgttttg
     1321 taccataggt cagaatccta caatccgcat taccaacaat gacatgcgta tcaccatatt
     1381 ttttcacaat cgtttcaagg tcagtcagtg gctcaaaaat aaagccgtct gcacctgcgt
     1441 tcgcaatatc gtcaacaaat tcagtgaaat ttccatccga gcaaaaaagg acctttattc
     1501 cttcggactt taagacatcc cacagttttt tatatcgtgg aaaaacatat ttcctgtacc
     1561 aggcaggatt gaaaattgca ccatttgacc agaccatatc atcgtgacag ataaacgcga
     1621 atggttttgc ctttgcccat gctttgaaat ttgcaagaga aacctgaaaa aacccttcaa
     1681 tcacataatc accaaatcta tcagggtcag taccaactgc ctcaagaaac atttcccatc
     1741 caaaacttgc aatgcaacca gaaacaatgg ttttataata accacctgga acaaggacat
     1801 caggatattc attttgagca ctttcaagtg tttgcttgaa atacattgcc cgttcatcaa
     1861 tatcaggtat tccatattcc tgtgctgcat caaagttcag tacttcatcc actgacttga
     1921 aaggacaggg tatggaattt ctataatc
//
LOCUS       DAORYU010000267         1947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_39534_length_1947_cov_4.102537, whole genome shotgun sequence.
ACCESSION   DAORYU010000267 DAORYU010000000
VERSION     DAORYU010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39534_length_1947_cov_4.102537"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="PLW07_07110"
     CDS             complement(<1..907)
                     /locus_tag="PLW07_07110"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HOL50336.1"
                     /translation="MAQANQQLSVSVLKRLEEKGIRESEIMLKVVADLDFSGQPAEIW
                     VIATDKRFIVIPEKGLQDEIDLDLQKIKKFRIRASIGSAYLQAKIDEFYIDIVRFTNA
                     LRFRFSRLIVQLDLLRQGKPVSDEYIQESHPMTCPVCGLPLQTEKASCPRCARQGPIL
                     FRVVFLLEPYLHWVIIVLILMFISVGLSLVPPKLTQILVDDVLTSRKHVEWLIWIVIA
                     LVSAELMRAFINMIVGTLTTSVGTLITFDLRKKLFKKLQELSIDYYDQQSVGTLMTRF
                     SSDVEAFHGFVTQVGQGFLLNLFLIA"
     gene            1053..>1947
                     /locus_tag="PLW07_07115"
     CDS             1053..>1947
                     /locus_tag="PLW07_07115"
                     /inference="COORDINATES: protein motif:HMM:NF012350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOL50337.1"
                     /translation="MEKPWIYDVAEIVKQLETDISKGLAEEDAKKRLVHYGYNKLAEK
                     KKRHPIFLFFEQFKSFIILVLLGAALIAGFLKEWIDTFAIIGIVILNSILGFFQEFKA
                     EKYLEALKKLFTQYSKVLRDGQIRVLKSELLVPGDIVELEAGDNIPADCRVVWHTTNF
                     AVQEASLTGESTPVVKTSIPLNEPETIIAERANMVYMGTSVSSGRAKCIVVDTGMKTE
                     LGKITEMIENTSHEKTPLQTRLESFGKFLVYICLLLVGIIFLIEWIRGGNFVDVFLTA
                     VSLAVAAIPEGLPAVVTIGLAL"
BASE COUNT          623 a          370 c          371 g          583 t
ORIGIN      
        1 ctgcgatgag aaaaagattc aacaaaaacc cctgtcccac ctgggtaaca aatccatgaa
       61 atgcttcaac atcagaagaa aatcttgtca tcagtgtacc tacagattgc tggtcataat
      121 aatcaataga aagttcctgc agttttttga aaagcttttt cctcaggtca aaagtaatca
      181 aggtaccgac agatgtcgta agcgttccaa caatcatatt gataaatgct ctcatcaatt
      241 ctgcactgac aagagcgatc acaatccata taagccattc aacatgtttt cttgaagtta
      301 aaacatcgtc aacaagaatc tgtgttagtt ttggaggtac caggctcaat cccacagaaa
      361 tgaacattaa aatcagaact atgatgaccc agtgaagata tggttcaagt aaaaatacaa
      421 ccctgaaaag aattggtccc tgtcttgcac accttggaca cgatgccttt tctgtttgaa
      481 gcggtaaacc acaaacagga caggtcatcg gatgggactc ctgaatatat tcatccgaaa
      541 ctggctttcc ctgtcttaat aaatcaagtt gtacaattaa acgactgaat ctaaatctca
      601 gcgcatttgt aaatctgacg atgtcaatat aaaattcatc aatttttgct tgaagatatg
      661 cgctgcctat tgatgctctg attctgaatt tttttatttt ttgaagatct aaatctattt
      721 cgtcctgaag acctttttcc ggtatgacaa taaaccgttt atcagtggct atcacccata
      781 tttctgcggg ctggccagaa aaatcaaggt ctgcaactac cttcaacatg atctctgatt
      841 ctctgattcc cttttcttca agacgtttta atacgcttac cgaaagttgt tgatttgcct
      901 gtgccatttt tccttttcta ccccataaca gaagaaaatg tcaatatgta agtataaaac
      961 acattgtaaa attttcaaca acctgctgga aacgaagatg gttatatggt attttaatat
     1021 ctgagatttt caaaaaaaag tggagataac agatggaaaa accctggatt tatgatgttg
     1081 cagaaatagt gaaacaactt gaaacagaca tttcaaaggg gctggctgaa gaagacgcaa
     1141 aaaaaaggct tgtccattat ggatacaaca aacttgccga aaaaaagaaa cggcatccca
     1201 tctttttgtt ctttgaacag ttcaaaagtt ttatcattct ggtacttttg ggtgctgccc
     1261 tgattgcggg ttttcttaaa gaatggattg atacatttgc tattattggt atagtaattt
     1321 taaactcaat tttgggtttt tttcaggaat tcaaggctga aaaatacctt gaggcgttaa
     1381 aaaaactttt tactcaatat tcaaaggttc tgcgcgacgg tcaaatccgc gttcttaaat
     1441 cagaattact tgttcccggg gatattgtcg aacttgaagc aggtgacaat atcccggcag
     1501 attgtcgtgt cgtatggcat accacaaact ttgctgtgca ggaagcaagt ttaactggag
     1561 aatccacacc tgttgtaaaa acaagcattc ctcttaatga gccagaaaca atcattgctg
     1621 agagagccaa tatggtatac atgggaacca gtgtttcttc tggtagagca aaatgtatcg
     1681 ttgtggatac cggaatgaag acagaactgg gaaaaatcac tgaaatgata gaaaacacat
     1741 cccacgagaa aacaccacta caaacaaggc ttgaaagttt tggtaaattt ctcgtataca
     1801 tatgtttatt gcttgtgggt atcatttttc taattgaatg gataagaggc ggaaattttg
     1861 ttgatgtttt ccttactgcg gtaagcctgg cagttgccgc gataccagaa ggacttcctg
     1921 cggtggtaac aattggtctt gcccttg
//
LOCUS       DAORYU010000268         1944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_39605_length_1944_cov_4.865008, whole genome shotgun sequence.
ACCESSION   DAORYU010000268 DAORYU010000000
VERSION     DAORYU010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39605_length_1944_cov_4.865008"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="PLW07_07120"
     CDS             complement(<1..830)
                     /locus_tag="PLW07_07120"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOL50338.1"
                     /translation="MLNLAVIGTGRYGTNIIHAFRQLESQGIATLVSICDTNQDVLTG
                     QCKKYNVQGYADYREMFDKEKIDAVAIATPDHLHRDIAVFAVNAGKHVLVEKPMDVTV
                     DGCLEMKKAAEKNNVLIQVDFHKRYDPYHQQLCKDIRSGKFGIIEYGYVHMEDRIEVP
                     SVWFSSWARYSSPVWFLGIHFIDLVRWCLQSNGKQVFATGRRIKLKKMGIDTYDSVSA
                     KVVFENSVSVTFDMCWILPEKFEAIVNQSIRFVGTEGIIECDTQDRGTRLCSTKNGME
                     T"
     gene            complement(858..>1944)
                     /locus_tag="PLW07_07125"
     CDS             complement(858..>1944)
                     /locus_tag="PLW07_07125"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOL50339.1"
                     /translation="IKEMVSRPFLHNYCFPSEIRANLVEKIALLAPEPLKKVFVLTTG
                     SETIECAIKLCRTAGQKISPAKKIIVSFQHGFHGRTLGSQMVGGIPALKEWIGNLDPD
                     MVQVPFPGDPRQQDRSFDVFINTLKEKGVNFDHICGVISETYQGGSAAFLPVEYAQKL
                     REFCNQHQAFLVFDEIQAGFGRTGKLWGFQHYGVIPDIFTLGKGISSGLPIAAVVGKP
                     EIMDLYEPGTMTSTHTGNPVCSASAIANIEYIVEKNLPEHAEKMGRVFEKELNRMKEK
                     FSSVGFVCGKGLVWALSIVKPGTIEPDGDTAFEIVKKCVEKGLLLFAPVGFGGASLKI
                     APPLVIKKDAIVEGCSVIEEAISETCS"
BASE COUNT          562 a          475 c          308 g          599 t
ORIGIN      
        1 gtttccatcc cattttttgt actgcacaat cttgttcccc tgtcctgcgt atcacactca
       61 ataatacctt ctgtaccaac aaatcttatg gattgattaa caatcgcctc gaatttttct
      121 ggcaaaatcc agcacatatc aaaagtgaca gacacgctat tttcaaatac aacttttgcg
      181 gatactgaat catacgtgtc aattcccatt ttcttcagtt taatacgcct acctgtggca
      241 aacacttgtt ttccgttgga ctgcaaacac catctcacca ggtcaatgaa atgaattccc
      301 agaaaccata ctggagaaga atatcgcgcc catgaagaaa accatacaga tggcacctca
      361 attctatctt ccatgtgaac ataaccatat tcaataattc caaattttcc tgacctgatg
      421 tccttacaca actgctgatg atatgggtcg tatctcttat gaaaatcaac ctgaatcaac
      481 acattgtttt tttctgctgc ttttttcatt tcaagacaac catcaacagt cacatccatt
      541 ggcttttcaa caagaacatg ttttcctgcg ttcaccgcaa aaacagcaat gtccctgtgg
      601 agatggtctg gtgtggcaat cgcaactgca tcaatttttt ccttatcaaa catttcccta
      661 taatccgcat agccctgtac attgtatttt ttacactggc cggtcaatac atcctgattg
      721 gtatcgcaaa ttgaaacaag tgttgcaatg ccctgggatt caagttgcct gaacgcatga
      781 ataatattgg taccatacct tccggtacca attaccgcaa ggtttaacat caatactcct
      841 gataaaataa cttacggtta tgagcaggtt tcagatattg cctcttctat gaccgagcaa
      901 ccttcaacaa ttgcatcctt tttgataaca agaggtggcg caattttcaa tgaagcccca
      961 ccaaaaccca ctggagcaaa aagcaatagt cctttttcaa cacacttttt cactatttca
     1021 aatgcagtgt ctccatcagg ttcaattgtt cctggcttca cgatactcaa cgcccacaca
     1081 agccctttac cacacacaaa cccaacagat gaaaatttct ctttcattct gttcaattct
     1141 ttttcaaata ctcttcccat cttttctgca tgctctggca gatttttttc aacgatatac
     1201 tcaatgttag caatagcaga cgcagaacaa acaggattcc ctgtgtgtgt acttgtcatt
     1261 gttcctggct cgtacaaatc cataatttca ggttttccaa caactgctgc aattggaagt
     1321 ccgcttgata ttccctttcc aagcgtaaat atatccggaa ttactccata atgctggaaa
     1381 ccccacaatt ttcctgttct tccaaaaccc gcctgtattt catcaaacac cagaaatgcc
     1441 tggtgctggt tgcagaactc tctcaatttt tgagcatatt caacagggag aaatgcagcg
     1501 cttccgccct ggtatgtttc agaaatcacg ccacagatat gatcaaaatt gacacctttt
     1561 tctttcaaag tattgatgaa aacatcaaaa cttctgtcct gctgtcgagg gtctccagga
     1621 aacggcacct gcaccatatc agggtcaaga ttcccgatcc attccttcaa agcagggata
     1681 ccgccgacca tctgagaacc aagtgttcgt ccatgaaatc catgttgaaa agaaacaata
     1741 atttttttcg caggactgat tttttgacct gctgttctac atagtttaat tgcgcattct
     1801 atggtttctg aacctgttgt aagaacaaaa acttttttca atggttcagg cgcaaggagt
     1861 gcgatttttt ccacaagatt tgctcttatt tcacttggaa aacagtaatt gtgaagaaaa
     1921 ggtcttgaca ccatttcttt gatt
//
LOCUS       DAORYU010000269         1918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_40331_length_1918_cov_4.064412, whole genome shotgun sequence.
ACCESSION   DAORYU010000269 DAORYU010000000
VERSION     DAORYU010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40331_length_1918_cov_4.064412"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PLW07_07130"
     CDS             <1..294
                     /locus_tag="PLW07_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013933034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HOL50340.1"
                     /translation="GGRQEIIDAFKKAVNSGYSVDKLDEISLQEFFYCPELPDVDLVI
                     RTAGEQRLSNFLIWQAIYAEFYFTEILWPDFSEEDFRKALDEFAKRKRKFGGL"
     gene            291..1424
                     /gene="dxr"
                     /locus_tag="PLW07_07135"
     CDS             291..1424
                     /gene="dxr"
                     /locus_tag="PLW07_07135"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOL50341.1"
                     /translation="MKNVVICGSTGFIGRATLDIIQRHPQRFRVFGLAAHTQKDLLIK
                     QIQKFKPRFVVFTGQHEKLLEIEGTEYFYGQEGLELLATHPETDILVMAISGLGGLVS
                     TLKALDAGKVIALATKEIIVCAGHLLDGRAGKILPVDSEHNAIFQIVDREKSGVSGIV
                     LTASGGPFLNYEGSLSMVTVKQVLNHPVWKMGQRITVDSATLMNKGLEFIEAFYLFGI
                     NRENISVVLHPQAIIHGFVKFCDGFVKAVISIPDMKYSINYCLNYPERIYAGLPELDI
                     ERMGTFTFQPVKPKMFPCFDLAIESLRKGGSYLTVLNAADEESVRLFLDGKIKFDAIP
                     YLIEKAMEKHTPVLLEKPQDILELDRNIRMEVKHFSENSRFSI"
     gene            1452..>1918
                     /locus_tag="PLW07_07140"
     CDS             1452..>1918
                     /locus_tag="PLW07_07140"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOL50342.1"
                     /translation="MFVIGILVVFGVVILIHEYGHFWVAKKLGVKVEKFSLGFGPKIF
                     SWKKNETEYLISLIPFGGYVKMAGEDAVESKFPDEYAALPPGKRALIVASGAIHNILI
                     AYVFLIPVLMLGISRYDGTLIGGLQKGFPAEIAGLKVGDRVISVNDIACKEWF"
BASE COUNT          582 a          283 c          464 g          589 t
ORIGIN      
        1 ggaggcaggc aggagattat tgacgctttc aaaaaggctg taaattcagg ctattctgtt
       61 gataaacttg acgaaatttc attacaggaa tttttttatt gcccagaatt acctgatgtt
      121 gaccttgtta taagaacagc tggagaacaa agattgagta attttttgat atggcaggcg
      181 atatatgcgg aattttattt tacagaaata ctgtggcctg atttttcaga agaagatttt
      241 agaaaggcac tggatgagtt tgcgaaaaga aaacggaaat ttggtgggtt atgaaaaatg
      301 tggtgatatg tggttcaaca ggttttattg gaagggcaac gcttgatatc attcaaaggc
      361 atcctcaacg atttcgggtt tttggtcttg cggcgcatac gcaaaaagat ttattgataa
      421 aacaaattca gaaattcaaa ccacgttttg ttgtttttac cggtcagcat gagaagcttc
      481 tggaaataga aggaaccgag tatttttatg gacaggaagg tcttgaattg cttgcaaccc
      541 atccagagac agatattttg gttatggcaa tttctggact tggtgggctt gtcagtactt
      601 tgaaggcgct ggacgctgga aaggtcattg cgcttgcgac aaaggaaatt attgtctgcg
      661 cggggcattt gctggacgga agggctggta aaattctccc tgtggacagc gagcacaatg
      721 caatttttca gattgttgac agagaaaaat caggtgtctc tggcatagtg cttactgcat
      781 caggaggacc cttccttaat tatgagggga gcctctccat ggtaacagta aaacaggtac
      841 ttaaccatcc tgtctggaag atgggacaga gaattacagt tgattcagca acattgatga
      901 acaaggggct cgaatttata gaggcatttt atttatttgg aataaacagg gaaaatatca
      961 gcgtggtttt acatccccag gcgattattc atggctttgt aaagttctgc gatggatttg
     1021 taaaggcggt aatatcaatt cctgacatga aatattcaat caattattgt ttgaattatc
     1081 ctgaaaggat ttacgcggga cttcctgaac ttgatattga aaggatgggt acgtttactt
     1141 ttcaaccggt gaaaccaaaa atgttcccat gttttgatct tgcaattgaa tcattgagaa
     1201 aaggtgggtc atatctaact gtgttgaatg ccgctgatga agaatctgta cgactttttt
     1261 tggatggtaa aataaaattt gatgctatcc cttatttaat tgaaaaagcc atggaaaaac
     1321 atacacctgt tctgttagaa aaaccacagg atattctgga actggataga aatatcagaa
     1381 tggaagtaaa gcatttttca gaaaacagca gattcagtat ttgaaaacat atatgtttct
     1441 aaggaggata aatgtttgta atcgggatac tcgttgtatt tggtgtggtg attcttattc
     1501 atgagtatgg ccatttctgg gttgcaaaaa aacttggggt aaaggtagaa aaattctcac
     1561 ttggttttgg tcccaaaatt ttttcctgga aaaaaaatga aactgagtat ttgatatcac
     1621 tgataccgtt tggagggtac gtgaagatgg caggtgaaga tgcagtggaa agcaaatttc
     1681 ctgatgagta cgctgcgtta ccaccaggga aaagggcatt aattgttgca tcaggtgcca
     1741 ttcataatat tttgattgca tatgttttct tgatacctgt gttgatgctt ggaatttcaa
     1801 ggtatgacgg aacactaata ggcgggttgc agaagggatt tcctgccgaa atcgcaggat
     1861 tgaaggttgg agatagagtt atttcagtga atgatattgc ctgcaaggaa tggtttga
//
LOCUS       DAORYU010000270         1872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_41772_length_1872_cov_6.908641, whole genome shotgun sequence.
ACCESSION   DAORYU010000270 DAORYU010000000
VERSION     DAORYU010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41772_length_1872_cov_6.908641"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="PLW07_07145"
     CDS             complement(<1..347)
                     /locus_tag="PLW07_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50343.1"
                     /translation="MRIEKQGFIFEAEKQSGTKRVCTFTGFFKHSNGKIFSSFRLGTR
                     KDSTDGNGVVCQSITGADWTIVFSGFKNQFDGHPGDIKITELFERPDGNISAFLSWFD
                     CSKSEKLYDASSDT"
     gene            complement(356..1336)
                     /locus_tag="PLW07_07150"
     CDS             complement(356..1336)
                     /locus_tag="PLW07_07150"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007779431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HOL50344.1"
                     /translation="MEGKKHVFVTRMIPEPAIELLKKTCEVEVNGEDRVLKKDELLDK
                     VKGKDAVLCLLTDTIDDEVLKAADRCKIFANYAVGYNNIDVESATRRKIIITNTPGVL
                     TDATGDLAWALLFAVARRVVEADAFTRQGKFKGWAPQLFLGQDITGKTLGVIGAGRIG
                     SNFAKKSKGFSMRILYYDVKRDLEFEQEAKAVFVDLDTLLKEADFVSIHVPLVPETRH
                     LIGARELSLMKKNAILINTSRGPVVDEKALVNALKEGKIWGAGLDVYENEPDIEPELL
                     NLANVVLLPHIASATYETRTKMALMAAENILAVFRGETPPNIVNPDALKK"
     gene            complement(1339..>1872)
                     /locus_tag="PLW07_07155"
     CDS             complement(1339..>1872)
                     /locus_tag="PLW07_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOL50345.1"
                     /translation="AFDLLIESTEEICRYAKEKNSMMVELEIFDYDIDKKSLIGPTNL
                     ARDFAKKIRGKFDNFGLLVDLSHIPLVKESSAQAIVPVKDFITHIHIGNCFISDRNSP
                     LYGDQHPPFGTPGSENDVEQLAEFLQVLLDTGFFDKKQRPIVSFEVKPLKDQDPDIVI
                     ANSKRTLNQAWLLVNEE"
BASE COUNT          525 a          421 c          311 g          615 t
ORIGIN      
        1 gtatcacttg aagcatcata caatttttca ctctttgaac aatcaaacca ggaaagaaaa
       61 gccgaaatat ttccatcagg tctttcaaac agttcagtta tcttaatatc ccccgggtga
      121 ccatcaaact gatttttgaa acctgaaaaa acaattgtcc agtcagcgcc tgttatggat
      181 tggcaaacaa ctccgtttcc gtcagtggaa tctttcctcg taccaagccg aaaacttgaa
      241 aaaattttcc catttgaatg tttgaaaaaa ccggtaaagg tacaaaccct ttttgtgcca
      301 gattgttttt ctgcttcaaa aataaaaccc tgcttttcta tgcgcataga ttttcctatt
      361 tttttaacgc atcaggatta acaatgtttg gtggtgtttc tcccctgaat acggcaagaa
      421 tattttcagc agccatcaat gccatctttg ttcttgtttc atatgtggcg cttgctatgt
      481 gaggcagtag caccacattt gcaagattta acaattcagg ttcaatgtca ggttcatttt
      541 catacacatc aagtcctgca ccccagattt ttccttcctt caaagcattc acaagcgcct
      601 tttcatcgac gacgggtcca cgggaggtat tgataagtat agcgtttttt ttcataaggg
      661 ataattccct tgcaccaatc aggtgtcttg tttctggaac aagtggtaca tgaattgaaa
      721 caaaatcagc ttctttcagc agtgtatcaa ggtcaacaaa cactgctttt gcttcctgct
      781 caaattcaag atctcttttg acatcgtaat agagtatgcg cattgaaaat ccctttgatt
      841 ttttcgcaaa gttgcttcct attcttccag cgcctattac gccaagtgtt tttcctgtaa
      901 tatcctgtcc gagaaacagt tgaggcgccc aacccttgaa tttaccctgc ctggtaaatg
      961 catcggcttc aacaactctt cgtgctacag caaacaaaag tgcccatgca aggtcacctg
     1021 tcgcatcagt gagtactcca ggtgtattgg taatgatgat tttccttcgt gtggcggatt
     1081 ctacatcaat gttgttatat ccaacagcgt agttggcaaa aattttacat ctgtcagctg
     1141 ctttcagtac ttcgtcatcg atggtatcag tcaaaagaca gagcaccgca tcttttcctt
     1201 ttactttgtc taaaagttca tcctttttaa gtactctgtc ttccccgttc acctcaacct
     1261 cgcatgtttt tttcaaaagt tcaatcgctg gttctggtat catccttgtt acaaatacat
     1321 gttttttccc ttccatattc actcctcgtt aacaagtaac catgcctgat taagtgttct
     1381 ttttgaattt gcaataacaa tatcaggatc ctggtctttc aaaggtttca cttcaaaact
     1441 cactatgggt ctttgcttct tatcaaaaaa tcctgtgtcc aaaagtacct gcaaaaactc
     1501 agcaagttgt tcaacatcat tttcactgcc aggtgttcca aaaggtggat gctggtcacc
     1561 atacagaggt gagttcctat cagatatgaa acaatttcca atatgaatgt gggtaataaa
     1621 atcctttact ggaacaatcg cttgagcaga agattctttt accagcggaa tatggctcaa
     1681 atcaaccaga agtccaaaat tatcaaattt tcctcttatt tttttcgcaa aatctcgtgc
     1741 cagatttgtc ggacctatca aacttttctt gtctatgtca taatcaaaaa tttcaagttc
     1801 caccatcata gaattctttt cctttgcata acggcaaatt tcttcagtgg attcaataag
     1861 aaggtcaaaa gc
//
LOCUS       DAORYU010000271         1871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_41807_length_1871_cov_6.645374, whole genome shotgun sequence.
ACCESSION   DAORYU010000271 DAORYU010000000
VERSION     DAORYU010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41807_length_1871_cov_6.645374"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..671
                     /locus_tag="PLW07_07160"
     CDS             18..671
                     /locus_tag="PLW07_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOL50346.1"
                     /translation="MKKGFTLIELLVVIAIIAILAAMLLPALSKARERARAAVCIANL
                     KQIALASKLYIDDYPGTLPRMPSVFYANGVLVGFCTGLYDLNYVKNWQVFKCPSDTRK
                     VDWSSNGQNASYSMNTGRDWEGSYGFPEVSADGTADEEGTIYIYCTRNWTGNTAVHPD
                     SWRQAYLAGNCGDHNGWVNIIFCDLHVGALGARYLTTQMGAPGCVWGFGPWSYSKND"
     gene            718..1443
                     /locus_tag="PLW07_07165"
     CDS             718..1443
                     /locus_tag="PLW07_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOL50347.1"
                     /translation="MKKKGFTLIELLVVIAIIAILAAMLLPALGRARAKARMTTCLNN
                     LKQIGLAVQLYLSDYNENWYPHLNGVDKLGYTWEWGLNYLPGKAVTAFLYTLIDKGYL
                     TGTMTYGTGAFYGDQYNDFLAASTGMVHCPCLDPYWENGYRRTGYPGWCDYAYNRNLP
                     VNAVKLGRVPKPAETLLFFESRAGEVDPLYPDPSYNQVWVQNWMYYGYGRHWQFELIN
                     AVFVDGHAASLTWNDFIKGGYPQ"
     gene            complement(1456..>1871)
                     /locus_tag="PLW07_07170"
     CDS             complement(1456..>1871)
                     /locus_tag="PLW07_07170"
                     /inference="COORDINATES: protein motif:HMM:NF019948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HOL50348.1"
                     /translation="ITKLIDFHTKYKYLLMLYSQKKLHQLGRIIAGYKKGRFEQTLSE
                     YEKIFYSVFERKPGIKKHYNVLQHIYGYFSNKIQPSEKRHFLSLMEKLKNEQIPLSAV
                     VEIAKNFALRFENFYLMSQKYFSPYPEQLEFSTSE"
BASE COUNT          554 a          341 c          431 g          545 t
ORIGIN      
        1 aaaaggaggt gagaaaaatg aaaaagggat tcacgcttat tgaactgctg gtggtgattg
       61 caatcattgc aatccttgct gcgatgctac tacctgcatt gagcaaggca agggaaaggg
      121 caagagcagc agtatgcatc gccaacctga agcaaatagc acttgcttca aaactgtaca
      181 ttgatgatta tccaggaaca cttcccagaa tgccaagcgt attttacgcc aatggagttc
      241 tggtcggatt ctgcacagga ctgtatgatt tgaactatgt gaaaaactgg caggttttca
      301 agtgcccatc tgatacaaga aaggtggatt ggtcaagtaa tggccagaat gccagttatt
      361 ccatgaatac tggtagggac tgggaaggat cttatggttt ccctgaggtt tcagcagatg
      421 gaactgctga tgaagaagga acaatttaca tctattgcac ccgcaactgg acaggaaata
      481 ctgctgtgca tcctgactcc tggaggcagg cataccttgc aggaaactgt ggagaccata
      541 atggatgggt gaatattatc ttttgcgatt tgcatgttgg agcccttggt gcaagatatc
      601 ttacaactca aatgggtgct cctggttgtg tctggggttt tggtccatgg tcttattcaa
      661 aaaatgatta atgatatgaa tgagaaattg aattttagca gaaggaggtg aaagaacatg
      721 aagaaaaagg gttttaccct gattgaactg cttgtggtca tagcaattat agcgatactt
      781 gcagcaatgc ttttaccagc actgggaagg gcaagggcaa aggcaagaat gacaacatgc
      841 ttgaataacc tcaaacagat aggacttgct gtccagttgt atctgagcga ttacaatgaa
      901 aactggtatc cacacctcaa tggggttgac aaacttggct atacctggga atggggactt
      961 aactatcttc ctggcaaggc agtgacagca tttctctata ccctcattga taaaggctat
     1021 cttacaggaa caatgaccta tgggacaggt gccttttatg gagatcagta taatgacttc
     1081 cttgcagcct caacaggcat ggtccactgc ccatgtcttg atccttactg ggaaaatggg
     1141 tatagaagga caggataccc tggctggtgc gattatgctt ataacagaaa cctgcctgta
     1201 aatgcagtaa aactcggcag ggttccaaaa ccagcagaaa cactattgtt ttttgaaagc
     1261 agggcaggcg aggttgatcc attatatcct gatccatctt ataaccaggt atgggttcaa
     1321 aactggatgt actacggcta tggaagacac tggcagtttg agctaatcaa tgctgtattt
     1381 gttgatggcc acgctgcttc tcttacatgg aatgatttta taaaaggcgg atatccgcag
     1441 taaaaaaaac agtccctatt ctgatgtgct gaattccagt tgctcaggat agggactgaa
     1501 gtatttttga gacataaggt aaaaattttc aaacctcaaa gcaaaatttt ttgctatttc
     1561 cacaacagcg ctgagtggaa tttgctcatt tttcagtttt tccataagag ataaaaaatg
     1621 ccttttctca gatggctgga ttttgtttga gaaatagccg tatatgtgtt gaaggacatt
     1681 gtagtgtttt tttattcccg gctttctttc aaatacagaa tagaaaattt tttcatattc
     1741 tgaaagagtt tgttcaaatc ttcccttttt gtatcctgca ataattcttc caagctggtg
     1801 taattttttc tgactgtaca gcatcaaaag atacttgtat tttgtgtgaa aatcaatcag
     1861 tttcgttatt g
//
LOCUS       DAORYU010000272         1862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_42111_length_1862_cov_3.314887, whole genome shotgun sequence.
ACCESSION   DAORYU010000272 DAORYU010000000
VERSION     DAORYU010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42111_length_1862_cov_3.314887"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PLW07_07175"
     CDS             <1..253
                     /locus_tag="PLW07_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50349.1"
                     /translation="GKVLEFSEHWIMVEGKGLIVSKGKITPVDMDAQVRTLIIPRNNI
                     SHIRLLPDDFDLSNIEIEESGIRYFIRVKNAPHTSIGEI"
     gene            259..1125
                     /locus_tag="PLW07_07180"
     CDS             259..1125
                     /locus_tag="PLW07_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50350.1"
                     /translation="MLYLGIDENGYGPIMGPLVVTGISGTGDNETWPEDIFDSKRLFS
                     SRSSFSKIEKIALSIFKIAYARFPFTYNELFEQTFECINITSGICWKNFPEIPFSTDK
                     KDIEIYATNLHNFLNKNNIRLVAIHSQVLCVKEFNNLCRKNVKKDFINYLMFEKIILK
                     HQKDYNHIAVKAGKIGGRNHYEKFLNNGFCNWEICCKKEDKKVSEYLVNKDSKQIRLS
                     FLNNIESVSFLGVLAGIYGKYIRELFMIAINKSLCTERFISGYRDAYTRQLVTQMNIE
                     DTGFEHCLCRIK"
     gene            complement(1129..>1862)
                     /locus_tag="PLW07_07185"
     CDS             complement(1129..>1862)
                     /locus_tag="PLW07_07185"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOL50351.1"
                     /translation="NARSPDVSNFKEQQLVNVVQEMSIAAGIKAPEVYVMEEASPNAF
                     ATGRDANHASVCVTTGLLDSMNREELQGVIAHELGHIRNRDILTMTVIAAITGAIVLL
                     SEWARRSMFYSGSYSGRARRSDKSSSSSGLLLLAILIITIIAPLIARLMALAVSRARE
                     YMADAASAEFTRNPLALASALGKISTHYDKVVDRASVGTAHLFISAPRNSKLNNSEST
                     FAEMFSTHPPIEKRIAILKHMAGVE"
BASE COUNT          602 a          361 c          348 g          551 t
ORIGIN      
        1 tggaaaggtg ctggaatttt ctgaacactg gattatggtt gaaggcaaag gtctcattgt
       61 atcaaaagga aaaatcacgc ctgtggatat ggatgcacag gtaagaacat tgataattcc
      121 gagaaacaac atctcgcata tcagactttt gcctgatgac tttgatttat caaatattga
      181 aatcgaagaa agcggtatcc gttattttat cagagtgaaa aacgcaccac acacaagcat
      241 cggtgaaatt taattctgat gttatatctt ggtattgatg aaaatggtta tggtcctatc
      301 atgggaccgc ttgttgttac aggaataagc ggtaccgggg acaatgaaac gtggcctgaa
      361 gatatttttg atagtaaaag attattttct tccaggagca gtttttcaaa aattgaaaaa
      421 attgccctct ctattttcaa aatcgcatac gccagattcc ctttcaccta taatgaactg
      481 tttgaacaga cgtttgaatg catcaatatc acctctggaa tttgctggaa aaatttccct
      541 gaaattccat tttcgaccga taaaaaagac attgaaatct atgccacgaa tcttcacaat
      601 ttcctgaata aaaataacat tcgtctggtt gctattcatt ctcaagtttt gtgcgtaaag
      661 gaattcaata atctttgcag aaaaaatgta aaaaaagatt ttatcaacta tctgatgttt
      721 gagaaaatta tactgaaaca tcaaaaagat tacaatcata ttgcggtgaa agcaggaaaa
      781 attggaggaa gaaatcatta tgaaaaattt ttaaacaatg gtttttgcaa ctgggaaatt
      841 tgttgtaaaa aagaagataa aaaggtttct gaatatctgg tcaacaaaga cagtaaacaa
      901 atccgtttga gttttttgaa taatattgaa tctgtctcat ttctcggagt cctggcaggt
      961 atttatggca agtatatcag ggaactattc atgattgcaa tcaataaatc cctttgcacc
     1021 gaaagattca tttctggata cagggatgcc tataccagac aacttgtgac acaaatgaac
     1081 attgaagata ctggatttga acattgtctt tgtagaatca aataaaactt attccacccc
     1141 tgccatatgt ttgaggatag caatcctttt ttcaatcggc ggatgcgtac tgaacatttc
     1201 tgcaaaagtg gactcactgt tattcaattt tgaattcctg ggggccgaaa taaaaagatg
     1261 ggctgtaccc acacttgccc tgtcaacaac tttatcataa tgcgtggata ttttcccaag
     1321 cgctgaagcg agcgcaagag gatttcttgt aaattctgct gatgctgcgt cagccatata
     1381 ctctcttgct ctggaaacag caagtgccat caatcttgct atcaatggtg caataatcgt
     1441 gataataagt attgcaagca gcaaaagtcc tgaagaagac gatgatttgt ctgaccttct
     1501 cgccctgcca gaataggagc cagaataaaa catgcttctt ctcgcccatt cagacagaag
     1561 cacaatggct cctgtaatag cggcgatcac tgtcattgtc aatatatcac gatttctgat
     1621 atgtcctaat tcatgagcaa tcacaccctg caattcttcc cgattcattg aatcaagcaa
     1681 tcctgtcgta acacacacag aagcatgatt tgcatccctt cctgtcgcaa aagcgttagg
     1741 cgacgcttct tccatcacat aaacttccgg cgcttttatt ccagcggcaa ttgacatttc
     1801 ctgaaccaca tttaccagtt gttgctcctt aaaattagac acatcaggcg accgggcatt
     1861 aa
//
LOCUS       DAORYU010000273         1860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_42162_length_1860_cov_4.916343, whole genome shotgun sequence.
ACCESSION   DAORYU010000273 DAORYU010000000
VERSION     DAORYU010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42162_length_1860_cov_4.916343"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..828
                     /locus_tag="PLW07_07190"
     CDS             73..828
                     /locus_tag="PLW07_07190"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HOL50352.1"
                     /translation="MMPENILKKKLKEGKPAVGAFVITAHPTIAEVMAVAGFDWLVFD
                     MEHGVIGIESVEAMAQAVSRTSTAPLVRVPWNDFVVIKQVLDTGVMGLIIPMVNTPEQ
                     AQQAVAATRYPPEGIRGIGPHRASGFGSWLPEYFERANESILVIVQIEHVNAVKNLEG
                     ILSVRGIDCVFIGANDLAASMGMLKNTSHPEVQKNIKYILGICKKKRIPCGLVATGSQ
                     IKQRIEEGFQFIAVGHDVSLLTSACKKALAEIK"
     gene            825..1817
                     /locus_tag="PLW07_07195"
     CDS             825..1817
                     /locus_tag="PLW07_07195"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HOL50353.1"
                     /translation="MKKRMKRYIVVNLDPEMHSLRNQDIEKLKVVNARFIELRQKDKM
                     VPLLKKADAILFTKTFISSDIIQNLEKCRIIVRFGTGLDNIDVRAATDKGIMVSRVVD
                     FCTEEVSNHAFMLLLACSRKLKKFSFTGVCDGPIDDFRHIGSIYDETLGLIGFGTIAR
                     SLAKKAKCSGMKILAYDPFVEKKVFEKYGGEKVSLKKILQESDYVSLHCPLTEHTHHL
                     IGEKELKLMKKTAYLINTSRGALIDEKMLIVALQKNWIAGAGLDVFEKEPPEQDNPLL
                     HMENVICTPHYAYYSDKAIETLKTIAVDEIIRAMKGQIPRNLYNPAVLGMKKRR"
BASE COUNT          621 a          302 c          416 g          521 t
ORIGIN      
        1 ctcaatacct aatgcaaaca aagaatatta tcctacaacg tatcgttaac taacatgtaa
       61 cagggagaaa aaatgatgcc agaaaatata ttgaagaaaa aactgaagga gggaaaacct
      121 gcagttggcg catttgttat tactgctcat ccaacaattg ctgaggtgat ggctgttgct
      181 ggtttcgact ggcttgtttt tgatatggaa catggcgtta tcggcataga atctgttgag
      241 gcaatggctc aggcagtttc cagaacatct acggcacctc ttgtgagggt tccttggaat
      301 gactttgttg taataaaaca ggtgctggac acaggagtta tgggattaat tattcctatg
      361 gtgaatacac ccgaacaggc gcaacaggca gtagcagcaa caaggtatcc gccagaagga
      421 atccgtggta ttggtcctca cagggcaagt ggttttggtt cgtggcttcc cgaatatttc
      481 gaaagagcaa atgagagtat ccttgtcatt gtccagatag agcatgtcaa tgctgttaaa
      541 aatcttgaag gcatattgag tgtcaggggt attgactgcg tttttattgg agccaatgac
      601 cttgctgcat caatgggaat gttaaaaaat acatcacatc cagaggttca aaaaaacatc
      661 aaatatattc tgggaatatg caagaaaaaa aggattcctt gcggacttgt tgccacaggt
      721 tcacagataa aacagagaat tgaagagggg tttcaattta ttgcagttgg tcatgatgtt
      781 tcactgttga cttcagcatg caaaaaggca ctcgcagaaa tcaaatgaaa aaaagaatga
      841 aacggtatat agtcgtgaac cttgatccgg aaatgcacag tttgagaaat caggatattg
      901 agaaattaaa ggtagtcaat gccaggttta ttgaattgcg gcaaaaagat aaaatggtac
      961 ctttattaaa aaaagcagac gcaattttgt ttacaaagac attcatatca tctgatatta
     1021 tacagaatct tgaaaaatgc aggattattg taagatttgg aacagggctt gataacattg
     1081 atgtaagggc agcaactgac aaaggtatta tggtttcaag ggttgttgat ttctgtactg
     1141 aagaggtgtc aaaccatgct ttcatgctgc tccttgcctg cagcagaaaa ttgaaaaaat
     1201 tttcttttac tggtgtttgt gatggaccaa ttgatgactt cagacatatc ggcagtattt
     1261 atgatgaaac cctggggctt attggttttg gaacaatagc acggtctctt gcaaaaaaag
     1321 caaaatgttc tggaatgaaa atacttgcat atgacccatt tgttgaaaaa aaggtttttg
     1381 agaaatacgg cggggaaaag gtttcgttga aaaaaatttt acaggagtcc gattatgttt
     1441 cgcttcattg tccgttgaca gaacatactc atcatctgat aggagaaaaa gagttaaaac
     1501 tgatgaaaaa aactgcttat ctgattaata cttcaagggg tgccctcatc gacgaaaaaa
     1561 tgctaatagt agcattgcaa aagaactgga tagcaggcgc tggtcttgat gtttttgaaa
     1621 aagagccacc agaacaggat aacccgcttt tgcatatgga aaatgtaatt tgtacaccac
     1681 actacgcata ttattcagat aaagcaatag aaactcttaa gacgattgca gttgatgaaa
     1741 ttatcagggc gatgaaaggg cagatacccc gcaatcttta taatcctgcg gtcttaggga
     1801 tgaaaaaaag aagatgaaaa tatacagatt ttgacttttc accgggttat tttcatctct
//
LOCUS       DAORYU010000274         1860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_42163_length_1860_cov_4.556233, whole genome shotgun sequence.
ACCESSION   DAORYU010000274 DAORYU010000000
VERSION     DAORYU010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42163_length_1860_cov_4.556233"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..662
                     /gene="pyrE"
                     /locus_tag="PLW07_07200"
     CDS             93..662
                     /gene="pyrE"
                     /locus_tag="PLW07_07200"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOL50354.1"
                     /translation="MTEHEILEIFRETGAVLEGHFLLSSGLHSNRYFQMARVLQFPHI
                     ARNLAQELVKRYTGNKVDVVIGPAIGGIVLSYALAEILGAKSIFAERENGLMCLRRGF
                     TIDRDENVLVCEDVITTGGSVSEVIDLVKTSGAKIAGVCCLVLRGQHCLEVPVESLLK
                     IEVKNYRQDECPLCEKKVPLVKPGSRLLK"
     gene            complement(665..997)
                     /locus_tag="PLW07_07205"
     CDS             complement(665..997)
                     /locus_tag="PLW07_07205"
                     /inference="COORDINATES: protein motif:HMM:NF018021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HOL50355.1"
                     /translation="MEIEKNELEEIVYQKLKKLPEFFQKKLENVEFFVEDGHSTRFLG
                     LYHGVPFGKRTNYNLVMPDKIIIYRRAFERACRNKKELEKKVEEVLLHEIGHYFGFNE
                     QQLRNLGY"
     gene            1027..1827
                     /locus_tag="PLW07_07210"
     CDS             1027..1827
                     /locus_tag="PLW07_07210"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF005719.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOL50356.1"
                     /translation="MIIVGERINATRKKIREALIKKDEKFFLQEAKKQIESGSDFLDL
                     NAGTGTGHEAEDLEWLVNLVQSAYPDVRLALDSSSTDVLAKCLPMVKNKPVMLNSVNA
                     EEKKLKEFLPLVKNFPDLYIVALTMDDSGIPSSFEKRIYIACTLYEKLTESGVKKENI
                     FYDALVQPVGTDTEAFNTYLKALCGIKERLPGTKSICGLSNVSFGLPKRSAINKYAFA
                     IAMYAGLDAVIIDPVEPGIIEARCIAGVLTGKDQFCMNYISSFREGKI"
BASE COUNT          587 a          312 c          400 g          561 t
ORIGIN      
        1 attaccaggg tttttcttga aacaaagttt ggcggtggaa gacacttgag aagaataaaa
       61 aaaattgaaa aattttgtaa cagggtacag aaatgactga acatgaaatt ctggaaattt
      121 tcagggaaac aggggcggtt cttgaggggc actttcttct ttcatcaggt cttcacagca
      181 acaggtactt tcagatggct cgtgttctcc agtttccaca cattgcaaga aatctcgcgc
      241 aggaacttgt gaaaagatat actggaaata aagttgatgt tgtgataggt cctgctatcg
      301 ggggaattgt tctctcctat gcgcttgctg aaattcttgg agcaaaaagt atttttgcag
      361 aaagagaaaa tggcctgatg tgtttaagac gaggttttac aatagataga gatgaaaatg
      421 tccttgtgtg tgaggatgtg atcaccacag gcggttctgt ttctgaggta atagaccttg
      481 ttaaaacgag cggcgcaaaa atcgcagggg tttgttgtct tgtattgcgg ggtcaacact
      541 gtctggaagt tcctgttgaa tctcttctaa aaatagaagt aaaaaattac agacaggacg
      601 agtgtccatt gtgtgaaaaa aaagtacctc tggtcaaacc aggcagtcgt ttgttgaaat
      661 aagttcaata accaagattt cttaattgtt gttcattgaa accaaagtaa tgtcctattt
      721 cgtgcagaag tacttcttca accttttttt caagttcttt tttatttctg caagcccttt
      781 caaaagcccg tctgtaaatg ataattttat ctggcatcac aaggttataa ttggtacgtt
      841 tgccgaatgg tacgccatga tacagcccaa gaaatcttgt tgagtgacca tcttcaacaa
      901 aaaactcaac attttcaagt tttttctgga aaaactctgg caattttttt aatttctggt
      961 aaactatttc ttcaagttca tttttttcaa tttccatagt aaaattttat cataccagga
     1021 gcacagatga taatagtagg agaaaggatt aatgcgacaa gaaaaaaaat tcgtgaagca
     1081 ttgatcaaaa aagacgaaaa atttttctta caggaagcaa aaaagcagat tgaatccgga
     1141 agcgattttc ttgacctgaa tgcaggaaca ggcaccggcc acgaagcaga agatcttgaa
     1201 tggttggtta atcttgttca gtcagcatat cctgatgttc gtctcgcgct tgacagttcg
     1261 agtacagacg ttcttgctaa atgtctgcca atggtgaaaa ataagccagt gatgctaaac
     1321 tctgttaacg cagaagaaaa aaagttgaaa gagtttttac cgttggtgaa aaattttcct
     1381 gatctttaca ttgttgcttt gaccatggac gattcaggca taccatcttc ttttgaaaaa
     1441 cgaatctaca ttgcctgcac actttacgaa aaattaacag aatccggggt gaaaaaggaa
     1501 aacatttttt atgatgctct tgtgcagccg gttggaacag atacagaagc attcaataca
     1561 tacttaaaag ctttatgcgg tataaaggaa cgattaccag gaacaaaatc aatatgtggt
     1621 ctttcaaatg tttcttttgg gttaccgaaa agaagtgcta ttaacaaata tgcatttgcc
     1681 attgccatgt atgcaggtct ggatgcagtg attatagatc ctgttgagcc aggaattatt
     1741 gaagcacgat gtattgccgg cgtgttaact ggtaaagacc agttttgcat gaattacatc
     1801 tcaagtttca gagaaggaaa aatttagtgt tcggggcgta gcgtagtccg gtttagcgca
//
LOCUS       DAORYU010000275         1846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_42579_length_1846_cov_4.694026, whole genome shotgun sequence.
ACCESSION   DAORYU010000275 DAORYU010000000
VERSION     DAORYU010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42579_length_1846_cov_4.694026"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /gene="rpsT"
                     /locus_tag="PLW07_07215"
     CDS             <1..195
                     /gene="rpsT"
                     /locus_tag="PLW07_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012447634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOL50357.1"
                     /translation="IKTSIKKVKKLILDGKEYEPTLKTAIKEIDRAVSKGVIHKNNAA
                     RKKNRLMKTVLKLTADIKQS"
     gene            complement(169..813)
                     /locus_tag="PLW07_07220"
     CDS             complement(169..813)
                     /locus_tag="PLW07_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50358.1"
                     /translation="MKQDNVKFLAGPHQLTTERIDYFVQKYSEQYENVEIKRYYGAEL
                     NPEIFVNELVANSLFNEHKIIIIHQMEQIEKKVWTEYIFPHLEKISDDMLVIFEGLSI
                     KTKPVGYEVETVQDAENLFRKIYQKSWKKNLTARDIYEISKFLKTNPYEFSVVIGVIE
                     RHLENLVMQKAISQQTFLNKLQNLLEIDFKLKSGRISNEPGWELLLLRLLDVSS"
     gene            complement(800..1276)
                     /locus_tag="PLW07_07225"
     CDS             complement(800..1276)
                     /locus_tag="PLW07_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50359.1"
                     /translation="MQKNLLLILNVLFLTGCGFHLINPAQNKVYVEFFSNHSLQPGIE
                     IFMTKNLKKTLAESPGFTLSSNKNVADIALGGTIQEFIRNPEFIAESDQIVMASYKVK
                     ILLTIMKDGKLTEKTFQQTYVLNLTRQLKTDELLDNISKKVSKDIYFQLIQFNETR"
     gene            complement(1248..>1846)
                     /gene="bamD"
                     /locus_tag="PLW07_07230"
     CDS             complement(1248..>1846)
                     /gene="bamD"
                     /locus_tag="PLW07_07230"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOL50360.1"
                     /translation="KRYPDFPEIEDVLKMQREISLEILQKKQLRLVEKFKDPSKKYEA
                     ITRTIESDPFNTETPPVALQLASRYAKSGEMEKAINLYQQVIRDFPGTQWAEKARYEM
                     LIHQIKSIPQGTTDVSLFSSVEKQIDLFIVDFPSSPYKQKLLDTRTQLRNEIAGRLFK
                     VAEIYSKNGKKKSAEIYYNKIKTLYPETEYAKKSTVNP"
BASE COUNT          544 a          330 c          317 g          655 t
ORIGIN      
        1 ataaaaacgt caatcaaaaa agttaaaaaa ctcattcttg atggtaagga atacgaaccg
       61 acactgaaaa cggcgataaa ggaaatagac agggctgttt caaaaggtgt gatacataag
      121 aataacgctg ccaggaaaaa aaacaggttg atgaagactg tgctcaaact aactgctgac
      181 atcaagcagt cgtaacagta acaattccca gccaggttca tttgaaattc ttcctgactt
      241 caatttgaag tcaatttcca ggagattctg cagtttattg agaaatgtct gctggctgat
      301 tgctttttgc ataacgagat tttcgagatg tctctctata actccaatca cgacagaaaa
      361 ttcataagga tttgttttca aaaattttga aatttcataa atgtccctgg cagttaaatt
      421 ttttttccag gatttttgat aaatctttct gaaaaggttt tcagcatcct gcacagtttc
      481 cacttcataa ccaacaggtt ttgtcttgat actgagccct tcaaaaatca ccagcatatc
      541 atcagagatt ttttcaagat gcggaaaaat atattctgtc catacctttt tttcaatctg
      601 ttccatctgg tgaataatga taattttgtg ttcattaaaa agagaatttg caaccagttc
      661 attcacaaag atttccggat ttaactcagc accgtaatat ctctttattt ccacattttc
      721 atattgttct gaatatttct gtacgaagta gtcaattctt tctgtggtca attggtgtgg
      781 ccctgcaaga aatttcacat tatcttgttt cattgaactg aatcagttga aaatagatat
      841 cctttgaaac tttttttgag atattatcaa gcagttcatc tgttttcaac tgtctggtaa
      901 gattaagaac ataggtttgc tgaaatgttt tttcagtcag tttgccatct ttcataatcg
      961 ttaaaagaat tttaactttg taagatgcca tgacaatctg gtcagattca gcaataaatt
     1021 cgggatttct aataaattcc tgaattgtgc cgccgagagc aatgtcagcg acatttttgt
     1081 ttgatgaaag ggtaaaaccc ggtgattcag ccagggtctt ttttaaattt tttgtcataa
     1141 agatttcaat gcctggctgt aatgaatgat ttgaaaaaaa ttcaacgtac actttgtttt
     1201 gtgcaggatt tatcagatga aaaccacaac ctgtgagaaa cagtacgtta aggattaaca
     1261 gtagattttt ttgcatactc tgtttctgga tacagtgttt ttatcttgtt atagtaaatc
     1321 tctgcgcttt ttttcttacc atttttgctg tagatttcag caaccttgaa aagcctgccc
     1381 gctatttcat ttctgagttg agttcttgtg tccagcaact tctgtttgta tggacttgaa
     1441 ggaaaatcaa caataaacag gtcaatctgt ttttcaaccg aagaaaaaag agaaacatct
     1501 gttgttcctt gtggaatact tttaatctga tgaatcagca tttcatatct tgccttttct
     1561 gcccattgtg ttccaggaaa atctctgatc acctgttgat acagatttat tgctttttcc
     1621 atttcaccgc tttttgcata ccttgaagca agctgcagcg ccactggcgg ggtttctgta
     1681 ttaaaaggat ctgattctat ggttctggta attgcctcgt attttttcga tgggtctttg
     1741 aatttttcaa ccagtcttaa ttgttttttc tgtaaaattt ccagtgaaat ctctctctgc
     1801 atcttcaaca catcttcaat ctctggaaaa tcaggatatc ttttta
//
LOCUS       DAORYU010000276         1841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_42759_length_1841_cov_3.500000, whole genome shotgun sequence.
ACCESSION   DAORYU010000276 DAORYU010000000
VERSION     DAORYU010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42759_length_1841_cov_3.500000"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /locus_tag="PLW07_07235"
     CDS             complement(<1..1006)
                     /locus_tag="PLW07_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50361.1"
                     /translation="MNKIIEDRIKGCLVGSYIGAELGFLRFAKPDLVKIEKPQEMFSR
                     QLEPIFQYQEQEKRITFRKVTPFIDIGVRAYLKYQHRVIPEEFALLVKNDEGLASPVF
                     NWDDIHTIQEVLKEGMHPRVSGLGVVPSANICAAMPAVGIYHATDPEYAYVDGVELAS
                     VVQCRTGADWAGLCAATIAACLDPDLSVDEIIEIVMKIGHKNARDVFYQMNKKIRVAM
                     SMAMGNEEEFLKWWYYNGGNNTERVTFNTLEFVLPLVKKYAQFPEKFLSLQIFAPNTG
                     SPVTGVIAGAISGALYGCQVFPEKWLKWAIPQISHWFGILDVVEKRLKKEKTIINTIE
                     T"
     gene            complement(1003..>1841)
                     /locus_tag="PLW07_07240"
     CDS             complement(1003..>1841)
                     /locus_tag="PLW07_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50362.1"
                     /translation="PEKLWCTEKGQIKAEKKDAKGVFEETLERYKQIKKHLEKLNPDT
                     KKQRAVAWLKEKVFSFRKPCELNPRFYLCESVEEFDVEMCLWWSQEGIFNHAYLFRNC
                     TMKKRTLPVVIALWDEGTNNIQNHIRWIRNKCNQGYAVMVCDVSGMGSISPNPIGGRT
                     LLEPRGTIHKFNDDLLWLDDSIAAMRVYDVIRACDMIENWHYLDKNQITCYACGYTGI
                     YGRLAAIIDKRIRKVEIENSMKNFAEIVENRYYNQYNVRSIVIPGILKYFDLSDFEEG
                     KK"
BASE COUNT          493 a          422 c          300 g          626 t
ORIGIN      
        1 aagtttcgat ggtattaatg attgtttttt ccttttttaa tcttttttca acaacatcca
       61 gaattccgaa ccagtgtgag atctgtggta ttgcccactt tagccatttt tcaggaaaca
      121 cctggcatcc ataaagggct cctgaaattg cccctgcaat cacaccagtg acagggcttc
      181 ctgtatttgg tgcaaaaatt tgaagggaaa ggaatttttc aggaaattgc gcatattttt
      241 tcacaagtgg caaaacgaat tcaagggtat tgaatgtaac cctttcagtg ttattcccac
      301 cattgtaata ccaccatttc aaaaattctt cttcattacc cattgccata ctcatggcta
      361 ctcttatttt cttattcatc tgataaaaca catcccttgc atttttgtgg ccaatcttca
      421 ttacaatttc aattatctca tcaactgaca gatctggatc cagacatgcg gcaattgttg
      481 ctgcacacaa tcctgcccag tcagcacctg ttctgcactg aacaacagat gccagttcaa
      541 ctccatcaac ataggcatat tctgggtctg ttgcatgata aataccaaca gcaggcattg
      601 ccgcacatat gtttgctgat ggaacaacac caagacctga aacacgcgga tgcatgcctt
      661 cctttaatac ttcctgtatt gtatggatat catcccagtt gaatacaggt gatgcaagac
      721 cctcatcgtt ctttaccaga agggcaaatt cttcaggaat gaccctgtgc tggtatttca
      781 gatatgccct tacaccgatg tcaataaatg gtgtcacctt tctgaatgtt attctttttt
      841 cctgttcctg atattgaaaa atcggttcaa gttgcctgga aaacatctcc tgtggttttt
      901 caattttcac aaggtctggc tttgcaaatc ttaaaaagcc aagttctgca ccaatgtatg
      961 agccgacgag acatcctttt atcctatctt caatgatttt gttcattttt tcccctcttc
     1021 aaagtcagaa aggtcaaaat atttcaatat tcctggtatc acgatacttc ttacattgta
     1081 ctggttataa tacctgtttt caacaatttc agcaaaattt ttcatgctgt tttcaatttc
     1141 aacctttctg attcttttgt caatgattgc tgcaagccga ccataaattc cagtatatcc
     1201 acaggcataa caggtaattt gatttttatc aagatagtgc cagttctcaa tcatatcgca
     1261 cgcccttatt acatcgtaca ccctcattgc tgcgatacta tcgtcaagcc atagaaggtc
     1321 gtcattaaac ttatgaatgg ttccccttgg ttcaagaagc gtccttccgc caatcgggtt
     1381 tggagagata gaacccattc cagaaacatc acagaccatc acagcatacc cctgattaca
     1441 cttatttctt atccagcgta tgtgattttg aatgttgttt gtcccttcgt cccataatgc
     1501 aataacaaca ggcagtgtcc tttttttcat cgtacaattt ctgaacagat acgcgtgatt
     1561 gaaaattcct tcctgggacc accacagaca catttcaaca tcaaattctt caactgattc
     1621 acacaggtaa aaccttggat ttaattcgca gggtttcctg aaagagaaaa ccttctcttt
     1681 taaccaggca actgcccttt gtttttttgt gtctgggttt aatttttcaa gatgtttttt
     1741 tatctgtttg tatcgttcga gggtttcttc aaatacacct tttgcgtctt ttttctctgc
     1801 tttgatctgt cctttttctg tacaccatag tttttcagga g
//
LOCUS       DAORYU010000277         1823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_43312_length_1823_cov_6.609163, whole genome shotgun sequence.
ACCESSION   DAORYU010000277 DAORYU010000000
VERSION     DAORYU010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43312_length_1823_cov_6.609163"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="PLW07_07245"
     CDS             <1..557
                     /locus_tag="PLW07_07245"
                     /inference="COORDINATES: protein motif:HMM:NF019736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50363.1"
                     /translation="EPYEAMKLYIHNFTHTSFACAGHLKNYRFIWESVSDQKIKKTVD
                     RAFFEIQKAINKKYGIPIEELDSYYKDLLERFLNKALGDTIARVAREPLRKLGPQDRF
                     IGAAKLCQQQDIVPEFVCFFVACCLHYNEATDTESQTLQHLLKEKGIDYVLENVSGLS
                     ASDSAFRLVKNYYLNFWKLWKKMI"
     gene            591..1628
                     /locus_tag="PLW07_07250"
     CDS             591..1628
                     /locus_tag="PLW07_07250"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent dehydrogenase"
                     /protein_id="HOL50364.1"
                     /translation="MPKAAVLKSIESLEIVERDIPAPREGEILIRVKSCAVCGTDIKV
                     FHHGHKHIVFPRVTGHELSGIIEKIGGNVKNLKEGDRVAVAPAIPCGQCYYCRRGWQS
                     MCDDLKAIGYHFDGGFAQYMIVPEVAVRNGCVNKIPDNVTFDQAAIAEPLACVINGQQ
                     LSRVEMGDTVLILGAGPIGCLHAELAKNIGASKVILTDVVQQRLDMAKFTGAITVNSK
                     ETNLKEFVSELTDGRMADRVIVAVGLAEAQEESLDLVAKRGSVNFFGGLPKESPFAKL
                     NTNVLHYGESFITGTHGSTPLHNKMALDFISAGKFHIDNYITRKIPLEQLLEGLSDAE
                     QKKALKIIVNP"
     gene            1640..>1823
                     /locus_tag="PLW07_07255"
     CDS             1640..>1823
                     /locus_tag="PLW07_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018040337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HOL50365.1"
                     /translation="MYRPTKQEAAKLLRQMMEIRQFEDKIMELLAKNIAEGGSHLYAG
                     MEAVAVGVISSLNPDDY"
BASE COUNT          614 a          319 c          418 g          472 t
ORIGIN      
        1 tagaaccata tgaagcaatg aaactttaca tccacaactt tacacatact tcatttgcat
       61 gcgcagggca cttaaaaaat taccggttta tctgggaatc tgtcagtgac cagaaaatta
      121 aaaaaacggt tgacagggcg tttttcgaaa tccagaaggc cataaataag aaatacggca
      181 tcccgattga agaacttgat agttattaca aggacctgtt agaaagattc ttgaacaaag
      241 cccttggaga tacaatagca agggttgcca gggaaccatt aagaaaactg ggtccgcagg
      301 atagatttat aggtgctgca aaattgtgcc agcagcaaga cattgttcct gaatttgtat
      361 gctttttcgt agcgtgctgc cttcattata atgaagcaac agacactgaa agccagacgc
      421 ttcaacatct tcttaaagaa aaaggtattg actatgttct ggaaaatgtt tctggacttt
      481 ctgcatctga ttctgctttt cgtctggtta aaaactatta cctgaatttt tggaaattat
      541 ggaaaaaaat gatataatgt ttttgacttt tttatgtaca ggggataaat atgccaaaag
      601 cagctgtact gaaaagtatt gaaagtcttg aaattgtcga acgagacata ccagcaccac
      661 gagaaggcga gatattgata agagtaaaaa gttgcgcggt ttgtggaaca gacatcaaag
      721 tattccatca tggacataaa catattgtat ttccaagagt cacaggtcat gaattaagcg
      781 gcattattga aaaaattgga ggtaatgtaa aaaacctcaa agaaggagac agggttgcag
      841 tagcaccagc gattccatgt gggcagtgct attactgccg aaggggatgg caatcaatgt
      901 gcgacgacct caaagccatt ggataccatt ttgatggagg gtttgcacaa tacatgattg
      961 tccctgaagt ggcagtaaga aatggatgtg taaataaaat tcctgacaat gtcacttttg
     1021 accaggctgc cattgcagag ccgcttgcgt gtgtcatcaa cggtcagcag ttgagcaggg
     1081 tggaaatggg agatacagta ctaattcttg gcgcaggtcc cattggatgt ctgcatgcag
     1141 aacttgctaa aaacattgga gcgtcaaagg tgattcttac tgatgttgtt cagcagcgac
     1201 tggatatggc aaaattcaca ggcgcaatca cagttaattc aaaggagaca aacctgaaag
     1261 aatttgtatc ggaattaaca gacgggagaa tggcagatag ggtgattgtg gcagtgggac
     1321 tggcagaagc acaggaagaa tctctcgacc ttgtcgcaaa aagaggttca gtgaattttt
     1381 ttggcggcct tccaaaagaa tctccgtttg ctaaattgaa tacaaatgtt ctccattatg
     1441 gagaaagttt tattacagga acacatggtt caacaccgct tcacaacaaa atggcgcttg
     1501 attttatttc agcagggaaa tttcacatag acaattacat aacaagaaaa ataccacttg
     1561 aacaattgtt agaaggatta agtgatgctg agcagaaaaa ggcactgaaa atcatcgtga
     1621 atccataaaa ggggaatata tgtacagacc tacaaaacag gaagcagcca aacttctcag
     1681 acagatgatg gaaatcagac aatttgagga caagattatg gaactgctgg cgaaaaacat
     1741 tgctgaaggc ggctcacatc tttacgcggg tatggaagca gttgcagttg gtgtcatatc
     1801 aagtttgaac ccagacgatt ata
//
LOCUS       DAORYU010000278         1799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_44144_length_1799_cov_3.447821, whole genome shotgun sequence.
ACCESSION   DAORYU010000278 DAORYU010000000
VERSION     DAORYU010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44144_length_1799_cov_3.447821"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="PLW07_07260"
     CDS             complement(<1..1051)
                     /locus_tag="PLW07_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50366.1"
                     /translation="MENNIYCNRVFLMTNLAFPYGKEFSFRVKVEPPELVQQPDKFQI
                     VIGKINFFTGKNTWSEWTKVTSEDIEAIKKGYPNLYMGRYPLVINIRVHPPVPDAKIY
                     LQYEVDGRQYSGEALYYGSDAMSVGTLQWEENGITKMGTMAQYNQKYWDQIDKAQASI
                     GEVKRPEKLLIVDRFIGGDSDFYDWKQGLDNLSRLGFNVFLMPADKKLRELLLQTPIK
                     KISYAVYNPPGYAFDFDEEKTSETAIKEWAKNIAKPYIDAGYDAKDMALFALSDEPGW
                     YFPSVFKHVNENPKNLERFHAYLKAQGLKPQDFGFKSWNDWDKVKPLGRSVANKDLPS
                     RKLYYWSCRFFPYVSN"
     gene            complement(1059..>1799)
                     /locus_tag="PLW07_07265"
     CDS             complement(1059..>1799)
                     /locus_tag="PLW07_07265"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016359031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HOL50367.1"
                     /translation="KNFVDSLSLDVAIVGAGPSGLMCGYRLAQSGYKVAVFERHLRAG
                     GGMPGGGMMFNSIVIQKEILRIFDELGITVRKYNDNLYVASSIETVSALCYRAMKSGV
                     KIFNLMSVEDVVIRENVVCGIVINWSSVVWSKLHVDPLSIKSKFVVDATGHDAEVCRI
                     VERKIGQKLQTPEGAVSGEKSMWAEKAERQILENTKQVYPGLFVCGMAANAVFGSPRM
                     GAIFGGMFLSGEKASELIDKELKKKGGL"
BASE COUNT          521 a          435 c          273 g          570 t
ORIGIN      
        1 cattggatac ataagggaaa aatctgcacg accagtaata aagttttctt gaaggtaagt
       61 ccttatttgc aacactcctt ccaagtggtt ttactttatc ccagtcattc cagcttttga
      121 acccaaaatc ctgtggtttc agtccctgtg cttttagata cgcatggaat ctttcaagat
      181 ttttcggatt ttcattgaca tgcttaaaaa ctgatggaaa ataccagcca ggttcatcag
      241 agagtgcaaa aagcgccatg tcttttgcat catacccagc atcaatatat ggctttgcaa
      301 tgttttttgc ccactctttt attgctgttt cagatgtttt ttcttcatca aaatcaaaag
      361 catatcctgg cggattgtac actgcatagg aaatcttttt gattggtgtt tgaagaagaa
      421 gctctctcag tttcttgtca gcaggcatga gaaacacatt aaagcccaat cttgataaat
      481 tatcaagtcc ctgtttccag tcataaaaat cgctatcacc accaataaac ctatcaacaa
      541 tgagaagttt ttcaggtctt ttcacttcac caatggatgc ttgtgcttta tcaatctggt
      601 cccagtattt ctgattatac tgagccatag ttcccatttt tgttattcca ttttcttccc
      661 attgaagagt tccaacactc atcgcatcag aaccataata aagcgcctcg ccagaatatt
      721 gcctgccatc aacttcgtac tggagatata tctttgcgtc aggaaccggc ggatgaacac
      781 gaatgttaat aacaagggga tatcttccca tataaagatt cgggtatccc ttttttattg
      841 cctcaatatc ttctgaagtt acctttgtcc attcactcca tgtattttta ccagtaaaaa
      901 agtttatttt cccaataaca atttgaaatt tgtctggttg ctgcactaat tcaggcggct
      961 ccaccttgac tctgaaagaa aattcctttc cgtatggaaa tgcaaggttc gtcatcaaaa
     1021 aaacccgatt gcaatagata ttattttcca tctggcaatc ataatccacc tttctttttc
     1081 agttctttat caatcaattc agacgccttt tctcctgata gaaacatacc gccaaatatt
     1141 gcacccattc ttggcgaacc aaacacagca tttgcagcca ttccgcagac aaaaagccct
     1201 gggtaaacct gttttgtgtt ttcaagaatc tgccgctctg ctttttcagc ccacattgat
     1261 ttttcaccag aaacagcccc ttctggtgtt tgaagtttct gaccaatttt tctttcaaca
     1321 atcctgcata cttctgcatc gtggcctgtg gcatcaacaa caaactttga ttttattgat
     1381 agcgggtcaa catgaagttt tgaccacacc acagaactcc agttaatcac aattccacac
     1441 acaacatttt ctcttatcac aacatcctca acgctcatca ggttgaatat ttttactcct
     1501 gatttcatcg cgcgatagca aagagcagat acagtttcta tggaactggc gacataaaga
     1561 ttgtcattat attttctcac cgtaataccc agttcatcaa aaattctcaa tatttccttc
     1621 tgaatcacga tactgttaaa catcattcca ccaccaggca taccaccccc tgctctcaga
     1681 tgcctttcaa acacagcaac cttatagccg gattgagcaa gcctgtaccc acacatcaat
     1741 ccagatggtc ctgctccaac aatcgcaaca tcaagagata aactatcaac aaaattctt
//
LOCUS       DAORYU010000279         1791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_44449_length_1791_cov_3.826037, whole genome shotgun sequence.
ACCESSION   DAORYU010000279 DAORYU010000000
VERSION     DAORYU010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44449_length_1791_cov_3.826037"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /locus_tag="PLW07_07270"
     CDS             complement(<1..1128)
                     /locus_tag="PLW07_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50368.1"
                     /translation="MNTEKLLAGWAEVDITPDMPVCLCGQFYARVSEGVRDPISATIL
                     VLEATGKNGREHAVMVSCDFVAIPAEFKKAVVAKTKTLVPEVDGTKIIMNATHTHTAP
                     EVGGDILRGTTGLEIKDIYGVELPVMKITQYIEFAASKIARGIQGAWEKRQETGIGYG
                     LGYAVVGFNRRVSYYDGSSAMYGNTSDTQFSHIEGYEDHTLNIMGTWDTQKNLTGLIV
                     NIACPSQVDEILFEISADYWHQVRKELRRRFGEHICILAQCSAAGDQSPHVLLCKQAY
                     QRMWELSGRTERQDIAVRIADGIQRVLGDIERDIKWQPEFTITSKKIRLEGYTVSLKE
                     VKQFSKEAEKARNEYERLKKEIENNPEIMKQSRWYRDITYHF"
     gene            complement(1144..>1791)
                     /locus_tag="PLW07_07275"
     CDS             complement(1144..>1791)
                     /locus_tag="PLW07_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009005133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOL50369.1"
                     /translation="RTKPLGIYCGSLYGRIRNWLTMEGACYLLVDDEALFDEIVETNA
                     EICYQAVKTTLETGVQFDFGHFWEDICFKNGPLISPDVFRKKIGPHYRRICDLLNKYG
                     IDIVSVDCDGWIDALVPVWLDNGVNTMFPIEVGTWNGNIKPWRKKFGKSIRGVGGMNK
                     VVFSKDKKAVYNEIRRLLELVELGGYIPCPDHRIPPDAKWELVVYYCQEMRKLTG"
BASE COUNT          478 a          449 c          283 g          581 t
ORIGIN      
        1 aaaatgatat gtaatatccc tgtaccatct cgattgtttc attatttcag gattattttc
       61 aatttctttt ttcaaccttt cgtattcatt tcgagctttt tctgcttctt ttgaaaattg
      121 tttcacttct ttcaaagaaa ccgtgtatcc ttcaagtcgt attttttttg aagtaatggt
      181 aaattcaggt tgccatttaa tgtccctttc gatatcacca agcacccttt gaatgccatc
      241 tgcaattctg acagcaatat cctgtctttc agttcttccg gatagttccc acattctttg
      301 atatgcctgt ttacacaaca gcacatgcgg agattgatct cctgcggcac tgcactgagc
      361 aagaatgcaa atgtgttcgc cgaatcttct tctcaattct ttacgaacct gatgccagta
      421 atcagcactg atttcaaaaa gaatctcatc aacctgactc gggcatgcaa tatttacgat
      481 caatcctgta aggtttttct gggtatccca ggttcccatg atgttaagtg tatggtcttc
      541 atacccctca atatggctga attgagtatc actggtgttt ccatacatcg ctgaagaacc
      601 atcataatag gaaacccttc tgttgaaacc cacaaccgca taacccagtc cataacctat
      661 accagtttct tgtctttttt cccaggcacc ctgaatgcca cgcgcaatct ttgaggcagc
      721 aaattcaata tactgtgtga ttttcatcac cggtaattcc actccataaa tatctttgat
      781 ttccagtccg gttgtgccac gaagaatatc tcctcccacc tcaggagcag tgtgtgtgtg
      841 cgttgcattc ataatgattt ttgttccatc aacctcagga acaagcgttt ttgtctttgc
      901 cacaactgcc tttttgaact ctgccgggat tgccacaaag tcgcaactga ccattacagc
      961 atgctcacga ccgtttttcc ctgttgcttc aagaacaagt atcgttgcac taataggatc
     1021 ccttacgcct tcagaaaccc ttgcgtagaa ttgaccgcac aaacacacag gcatatctgg
     1081 cgtaatatca acttcagccc atccagcgag aagtttttct gtgttcatat ttcccctcca
     1141 tattcaaccg gttaattttc gcatttcctg gcaataatat acaacaagtt cccactttgc
     1201 atcaggagga attctgtggt cagggcatgg aatataacca ccaagctcca caagttccag
     1261 tagtcgcctt atttcattat aaactgcctt tttatctttt gagaaaacaa ccttgttcat
     1321 tccacccaca cccctgatgc ttttaccaaa tttttttctc cacggcttga tgtttccatt
     1381 ccatgttcca acctcaatgg gaaacatggt gtttactcca ttatccagcc atacaggaac
     1441 aagggcatca atccatccat cacaatcaac actgacaata tctataccat acttattcaa
     1501 caaatcacag attcttctgt aatggggccc aattttcttt ctgaaaacat ctggcgaaat
     1561 taacggtcca tttttgaaac agatatcttc ccagaaatgt ccaaagtcaa attgtacacc
     1621 tgtttcaagg gttgttttta ctgcctgata acatatttca gcgtttgttt ccacaatttc
     1681 gtcaaaaagt gcttcgtcgt ccacaagaag ataacaggcg ccttccattg tcaaccagtt
     1741 tctaatcctt ccgtagagac ttccacaata aattcctaat ggcttggttc t
//
LOCUS       DAORYU010000280         1790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_44482_length_1790_cov_6.648415, whole genome shotgun sequence.
ACCESSION   DAORYU010000280 DAORYU010000000
VERSION     DAORYU010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44482_length_1790_cov_6.648415"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="PLW07_07280"
     CDS             complement(<1..340)
                     /locus_tag="PLW07_07280"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOL50370.1"
                     /translation="MKDTKIRVVLIGCGAISAAWLVPALKEKEIEIIGLVDVNKNNAE
                     TKAHQFKLNNVLVSTSFDQALKLKPDVVFNCTPPNIHYETTIKALKNGCHVLVEKPLA
                     HSLYHAKKMVE"
     gene            complement(361..>1790)
                     /locus_tag="PLW07_07285"
     CDS             complement(361..>1790)
                     /locus_tag="PLW07_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50371.1"
                     /translation="ETDFNFGKYGLAYYLMAKVLWNPEMSLQELETIRDRWFQKAFGS
                     AWKEMKQYYDFMLVDNLPLNGPSSWAKAIRMIDAASKKIDPMKEPDVQKRIDDVKQYW
                     YYHYLEESGKATPTSKEMQEYVRKGQMSYMVAMHAVVRRVFGTHVVKNVAGEFTGGPA
                     HYTHEETEKWWNQVLDFWKPVPVTYFKDTPLINGKKASEIDLNDLVSVKEFQKEFTSP
                     CNYYRTTPYDAPFAYNSCYQSVEFLTVAKSAGDIIGFKLFWPYKPNDSAYSQKDVSYG
                     VDYWDTKEKKWVPVIGETMTQQPSQEVTLPDGSKKQIVDVSIKANIPGTYRFNIGRGG
                     NLASLTTPYYDIQTGKHSKPYPLTYFQNQGGLTQQPVYFYIPKGTKSLDFEVFDTFHG
                     KKLTLYTGLFPQEKEKIREIDISKQAVYTIKLNPGEDGTIARIQSNGFAFPYFYSIPH
                     LWAKSPAALVLPRAIAEADGWTIDE"
BASE COUNT          464 a          368 c          345 g          613 t
ORIGIN      
        1 cttctaccat tttttttgca tggtaaagag agtgggcaag gggcttttca accaggacat
       61 gacatccgtt tttgagtgcc tttattgttg tctcataatg aatgttaggc ggggtgcaat
      121 tgaaaacaac atctggtttc aatttaagcg cctggtcaaa gctggtactg accaatacat
      181 tgttcagttt gaactggtgt gcttttgttt ctgcattgtt cttgttgaca tcaacaagcc
      241 ctataatttc aatttctttt tctttcagag caggtaccag ccatgctgcg cttattgcac
      301 cacaacctat cagaactact ctaatttttg tatccttcat cggatattca ttctacaaat
      361 ttattcgtct attgtccagc cgtctgcttc tgcgattgcc cttggcagaa ccagggctgc
      421 aggtgattta gcccataagt gtggaattga atagaaatat ggaaaagcaa aaccatttga
      481 ttgaattctt gcgatggtgc catcttctcc tggattcaat ttgattgtat atacagcctg
      541 tttgcttatg tctatctccc ttattttttc tttttcctga ggaaataatc cagtataaag
      601 tgtgagtttt ttaccgtgaa aagtatcgaa aacttcaaaa tcaagacttt tggtaccctt
      661 ggggatgtaa aaatatactg gttgctgggt taatccaccc tgattctgaa aatatgtgag
      721 tggatacggt tttgaatgct tgcctgtttg aatgtcgtaa tagggggttg taagcgatgc
      781 aaggttacca ccacgtccaa tgttgaacct gtatgtgcca ggtatgtttg ccttaatgga
      841 aacatcaaca atctgttttt ttgaaccatc tggaagcgtt acttcctgcg atggttgttg
      901 tgtcatggtt tcaccaatga ctggaaccca ttttttttct tttgtatccc agtaatcaac
      961 accataggaa acatcttttt ggctataagc gctatcattt ggtttgtaag gccagaacaa
     1021 tttaaatccg atgatatcac cagcagattt agccactgtc aaaaattcca cggattggta
     1081 acagctgtta tacgcaaaag gggcatcata aggggttgtg cggtaatagt tgcatggact
     1141 ggtaaactct ttttgaaatt cttttactga aacaaggtca ttgaggtcta tttctgatgc
     1201 ttttttacca ttgatgagag gggtatcctt aaaatatgtt actggaacag gtttccagaa
     1261 atcaagcacc tgattccacc atttttctgt ttcttcgtgg gtataatgag caggcccacc
     1321 ggtaaattct cctgctacat tcttgacaac atgggtccca aaaactcttc tcaccaccgc
     1381 gtgcatcgca accatataag acatctgtcc cttccgcaca tattcctgca tttcctttga
     1441 tgttggcgtt gcctttccgc tttcttcaag ataatggtag taccagtatt gtttcacatc
     1501 atcaattctt ttttgaacat caggttcttt catagggtca attttcttac tggcagcatc
     1561 tatcattctt attgcttttg cccacgaact tgggccatta agaggcagat tatcaacaag
     1621 cataaaatca tagtactgtt tcatttcttt ccatgcgctt ccaaatgcct tttgaaacca
     1681 cctatctctg atggtttcaa gttcctgaag ggacatttca ggattccaca aaactttagc
     1741 cattaaataa tacgcaagtc catatttgcc gaagttgaaa tctgtttcgc
//
LOCUS       DAORYU010000281         1784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_44692_length_1784_cov_4.305379, whole genome shotgun sequence.
ACCESSION   DAORYU010000281 DAORYU010000000
VERSION     DAORYU010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44692_length_1784_cov_4.305379"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1424)
                     /locus_tag="PLW07_07290"
     CDS             complement(<1..1424)
                     /locus_tag="PLW07_07290"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOL50372.1"
                     /translation="MTKLLVSLIIFFFSLPLFSADIDARNYINYKLPDCGIQKAIEEC
                     YASGGGTVQIPEGVFRLRRGLVLRNNVNIEGKGIDKTVFIFGKEIFKFNVVKAEGNKI
                     FLEAIPEKMDAGSAITLCRIFPPSWYGEPRPAYVKEIDRQDKSIVVEAPYGMVQIKSG
                     TACIIFGDSAAPDRDITKGDTEIFLKHAGIFQPGEEIAIGEPPNESMNAHAFIKEIKG
                     NKLILEAPVSINFKMWPADKEIGNKKINALIWGLFPLIHGANVENIRISNLTIRGNVK
                     DNAYTTQTRYTLGGIHIYNGKNIIIEKVKVYDWHSDGISLQEGNSCIVRDCEISGCIG
                     NGLHPGTGLKNSILERNKIVENEQGIYFCWNNTGHKILNNIIERNKSHGIGGLGNPGD
                     TSNLIEGNSIVANGGAGIEINGGRKSNNVIANNIIKNNSQSQPGKYPGIMIYASTEDA
                     MNYTIRNNTIADTQETKTQYIGIE"
BASE COUNT          468 a          390 c          289 g          637 t
ORIGIN      
        1 tcttcaatac cgatgtactg agtttttgtc tcctgtgtat cagcaattgt gttattccgt
       61 atcgtgtagt tcatcgcatc ttctgtggaa gcgtaaatca taattcctgg atacttccct
      121 ggttgcgatt gtgaattgtt cttgataatg ttgtttgcaa tcacattgtt tgattttctt
      181 ccaccattga tttcaatacc tgcaccgcca ttggcaacaa tgctgtttcc ctctatcagg
      241 ttactggtat ctccgggatt accgagccca ccaataccat ggcttttgtt cctttctatg
      301 atgttgttca gtattttgtg tcctgtattg ttccagcaga aataaatgcc ctgttcgttt
      361 tcgacaattt tatttctttc caatatgctg tttttcaatc ctgttcctgg atgcaaccca
      421 tttccaatgc atcctgaaat ttcacaatcc cgcacaatgc aactgtttcc ttcctgcaaa
      481 ctgataccat cgctgtgcca gtcataaact ttcacctttt caataataat atttttcccg
      541 ttgtaaatgt gtattcctcc aagggtatat cttgtttgtg ttgtgtaggc attatccttt
      601 acattacccc tgattgtcag attgctgatt ctgatatttt caacatttgc tccatgtatt
      661 aaaggaaaca gcccccatat caaagcattg attttcttgt tccctatttc tttgtcagca
      721 ggccacattt tgaaatttat tgaaactggc gcttcaagaa tcagtttgtt tcccttgatt
      781 tcttttatga aggcgtgtgc attcatggat tcgtttggtg gttctccgat agcaatttct
      841 tctcctggct ggaaaatacc tgcgtgtttg agaaaaattt cagtatctcc ttttgtgata
      901 tccctgtcag gtgctgctga atcaccaaaa atgatacacg ctgtaccgga ttttatctga
      961 accattccgt atggtgcttc aacaacaatt gacttgtcct gtctgtctat ttctttcaca
     1021 taagcaggtc ttggttctcc ataccatgaa ggtggaaata tcctgcaaag tgtaatggca
     1081 cttcccgcat ccattttctc cgggattgct tcaagaaaaa ttttgttacc ttcggctttc
     1141 acaacattga atttgaatat ctctttgcca aagatgaaaa cagttttatc aatgcccttt
     1201 ccttcaatat tcacattatt tctcaaaaca agtccccttc ttaatctgaa tacaccttca
     1261 ggaatttgca ctgtgccgcc gccagacgca taacattctt caatggcttt ctgaataccg
     1321 caatcaggaa gtttatagtt tatgtaattt cttgcgtcaa tatcagcgga aaaaagcgga
     1381 agagagaaga agaaaattat caatgatacc agtaattttg tcacgcattc tccctgtaaa
     1441 atcaattggt gatgaataaa cttcaatagt atcctgattc aaaatactgt acaattttgc
     1501 gtgtcagaat ttcccttttc caatttttat tttaattcct tcagagaata attcaagttt
     1561 tactcatttt attaattctt agggtcagat tttaacaacc tgcatatcat acaatgtcct
     1621 ttgctcatcg tattgacaca tccccgggaa aatacattga accttatcgc tctccattag
     1681 gatggcatgg gaaggtaaaa agcaaagccg gtgttagtaa tactccatat caatcaagtc
     1741 ctatcgcttt tctcaaaatc aaaatcacat ctgaaatgtc caca
//
LOCUS       DAORYU010000282         1764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_45438_length_1764_cov_4.156817, whole genome shotgun sequence.
ACCESSION   DAORYU010000282 DAORYU010000000
VERSION     DAORYU010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45438_length_1764_cov_4.156817"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PLW07_07295"
     CDS             <1..188
                     /locus_tag="PLW07_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50373.1"
                     /translation="GMDQQLIRDLFGEPDETYLSETGMFEMWYYENFAFGFDSEGVLV
                     KFFGPQDMRIKKKKTWR"
     gene            192..1247
                     /locus_tag="PLW07_07300"
     CDS             192..1247
                     /locus_tag="PLW07_07300"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOL50374.1"
                     /translation="MEKLKWGVIGSGGIAFRRTIPEGIIPADNAELVAVCDVNEEMAR
                     KSGETFHVRYYTDEDELIADSEVEAVYIATPAYLHFKQAEKAVKAKKHVLIEKPMTLK
                     VEEGKKLVALARRKKVKIGVDFMMRFNTINRKIREMVLNGELGTIVVARAQLSCWYPP
                     IKGAWRQIPSLGGGGSFIDMGCHCMDLLEFILDSKIKEITCMLDTLVHNYPVEDTAIA
                     MARFANGAIGIIDSCFSIPDNSSKNVLEIYGSKGSVIAMGTIGQSPDGDATAYLETET
                     KDYDAQQQRKLLSTSKKIEAEPYNTYRAQIESFSRAVIEDTEVFNSGKDGVHNQALVI
                     AAYKAAKTGKVQKVSLK"
     gene            complement(1253..>1764)
                     /locus_tag="PLW07_07305"
     CDS             complement(1253..>1764)
                     /locus_tag="PLW07_07305"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOL50375.1"
                     /translation="VEDEASSLLEYENGAWGYYYTTTNEVPAISYLEIAGDKGKIIYN
                     GASLRFFATKQPISQFTFNNKSMWASVETKEKALRIPKKEASHGAIIKNFCRAIIKGE
                     TLVSPGIEGINSVEFINAILLSGYKGKPVETPVDRDEYDAFLQDMIKKSKDKTTVRVQ
                     IQTDPNLKK"
BASE COUNT          551 a          314 c          393 g          506 t
ORIGIN      
        1 ctggaatgga ccagcaatta ataagggacc tttttggaga accagacgag acataccttt
       61 cagaaacagg aatgtttgaa atgtggtact atgaaaactt cgcatttggc tttgatagtg
      121 agggcgtgct ggtgaaattt tttggtccac aggatatgag gataaaaaag aaaaaaacat
      181 ggaggtaaaa gatggaaaaa ctgaaatggg gtgttattgg ttctggtgga atagcattca
      241 gacgcacaat tccagaaggt attattcctg cagacaacgc tgaacttgtt gcggtttgtg
      301 atgtaaacga ggaaatggca agaaaatcag gtgaaacatt tcatgtgaga tattatacag
      361 atgaggatga actgattgcg gattcagaag ttgaggcggt ttatattgcc acgcctgctt
      421 atttgcattt caaacaggca gaaaaggcag tcaaggcgaa aaaacatgtt ctgatagaaa
      481 agccgatgac cctgaaggtt gaagagggga aaaaactcgt tgcgcttgcc agaagaaaaa
      541 aagtgaagat aggagttgac tttatgatga gatttaatac catcaacagg aaaattcgtg
      601 agatggtatt aaacggagag cttggaacaa ttgtcgtggc aagagcgcaa ttatcctgct
      661 ggtatcctcc tatcaaaggc gcctggcggc agattccatc tcttggtgga ggaggttctt
      721 ttattgatat gggttgccat tgtatggatc ttcttgaatt tattcttgac agcaaaatca
      781 aagagattac ctgcatgctt gatacacttg ttcataacta tccagtggaa gatacagcga
      841 ttgcaatggc aaggtttgca aatggagcaa tcggcattat agacagttgt ttcagcatac
      901 ctgacaattc ttcaaaaaat gttcttgaaa tttatggaag taaaggcagt gtaatagcga
      961 tgggtacaat tggccaatct cctgatggcg atgctactgc gtatcttgaa acagaaacaa
     1021 aagattatga tgctcaacag caacgcaagc tgctttccac aagcaaaaaa attgaagcag
     1081 aaccctataa tacctacagg gcgcagattg agagtttctc cagagccgtc atcgaagaca
     1141 cagaagtgtt caattcagga aaagatggtg tccacaatca agcccttgtc attgctgcct
     1201 ataaagccgc aaaaaccggc aaggttcaga aagtatcctt aaaataattt gtttattttt
     1261 taagatttgg gtcggtttgt atctgtaccc tgactgttgt tttatctttt gattttttaa
     1321 tcatatcctg caagaaagca tcatactcat ctctatccac aggagtttca accggtttac
     1381 ccttataccc tgaaagcaat atcgcattga tgaactcaac agaattgatt ccttcaattc
     1441 caggagaaac aagggtttcg cccttgatta ttgccctgca gaaatttttg ataattgcac
     1501 catgtgatgc ttcttttttg gggatacgca gggctttttc tttcgtttca acagaagccc
     1561 acatactttt attgttgaag gtaaattggc tgataggctg ttttgttgca aaaaatcgca
     1621 gagatgcccc attataaata attttccctt tatccccggc aatttcaaga taagaaatgg
     1681 caggaacttc attggtagtt gtgtaatagt atccccatgc gccattttca tactcaagaa
     1741 gggaacttgc ttcatcttca actt
//
LOCUS       DAORYU010000283         1751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_45899_length_1751_cov_3.176887, whole genome shotgun sequence.
ACCESSION   DAORYU010000283 DAORYU010000000
VERSION     DAORYU010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45899_length_1751_cov_3.176887"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            247..1107
                     /locus_tag="PLW07_07310"
     CDS             247..1107
                     /locus_tag="PLW07_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50376.1"
                     /translation="MLYHNKFGAVKHAVVLLTILLLVFLMPSFCANDYSKILNRKIFA
                     DPPPPPEKPVRSILKPAPVPSLDSLISVKGIIYSPDGESRAIIEIVAKRVETLATEGD
                     IIENARLIRIDENNLVFYYDDKEITLNLSKPQIDSKSGIAIKDTSVKLASGQPSTTAT
                     SSATGTVVASSSSSIPVPQQPKNINLNALIDQVRSDPTAMSNISVTPYIEDGKVSGFT
                     VNRIPEGGMYSQIGVQPGDVIKRVNGTLIDSLSKAYAVYNNIVSSQTKLVTVEILRNG
                     QPMILTYRLD"
     gene            1112..>1751
                     /locus_tag="PLW07_07315"
     CDS             1112..>1751
                     /locus_tag="PLW07_07315"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOL50377.1"
                     /translation="MHLAFFQEKLEGKKVRCRLCNHFCIIQDGERGLCNVRVNRNGEL
                     FSLVYGYPVAKNIDPIEKKPFFHFLPGSSAFSISTVGCNFKCGFCQNFEISQVSSDYT
                     PYGATISPAEIVRYAKKYNCDSISYTYTEPTVYFEFAYECAVLAKQAGLKNNFVTNGY
                     MSAEALKKIAPYLDAANVDLKGDENFYKNLCGAHLAPVLKNIQAMKEYGIWVE"
BASE COUNT          551 a          309 c          394 g          497 t
ORIGIN      
        1 cccccaaccc ctcttttata aagaggggag ctatgtgaag gaagtttata aagaggggag
       61 tcatttaagt ggggttataa agaggggagc aatataagtg agatttatga agaggggagt
      121 aatataaagg aaatttatga agagggggtg atgggaaatt tataaagaac ggaggaaaat
      181 ggcgattttt aaagagaggg tttttgaatt tttcaactta tgctataata ttttcggggt
      241 gaagcgatgc tgtatcacaa taagtttgga gcagtgaaac acgcagttgt tcttttaact
      301 atcctattgc tggtttttct tatgccttcc ttctgcgcaa atgattattc taaaattttg
      361 aacagaaaaa tttttgctga cccgccacca ccgcctgaaa aaccagttcg ctcaattttg
      421 aagccagcgc cagtgccatc ccttgacagt ttgatttctg taaaaggaat catctacagt
      481 cctgatggcg agtcaagggc aattattgaa attgtcgcaa aaagggttga gacacttgca
      541 actgaaggag atattattga gaacgcccgt ttaataagaa ttgatgagaa caatcttgtt
      601 ttttattacg atgacaagga aattacgctg aatctttcaa aaccacagat tgacagcaaa
      661 tcaggaattg caatcaagga tacttcagta aaacttgcgt ccggtcagcc atcaactaca
      721 gcaacttcat ctgcaactgg cactgttgta gcatcatcat cgtcatctat tccagttcca
      781 cagcagccaa agaacatcaa tcttaatgct cttattgacc aggtaaggtc tgaccctact
      841 gccatgtcaa atatcagtgt aacgccatat attgaggatg gaaaggtgag tggttttact
      901 gtgaatagaa ttcctgaagg tggaatgtac tcacagattg gcgtgcagcc aggcgatgtg
      961 ataaaaaggg tcaatggtac tttgattgat agcctttcaa aggcatacgc tgtgtacaac
     1021 aacattgtca gcagccagac aaaacttgtt actgtggaaa tcttgagaaa cgggcaaccg
     1081 atgattctta catacaggtt ggattagaac catgcatctg gcattttttc aggaaaaact
     1141 tgaagggaaa aaagtcaggt gtcggctgtg caatcacttc tgtattattc aggatggtga
     1201 gcgcggatta tgcaatgtca gggtaaatcg taatggagaa ctattttcgc ttgtctatgg
     1261 ataccctgtc gcaaaaaaca ttgacccgat agagaaaaag ccatttttcc attttcttcc
     1321 aggcagttct gccttctcaa tttcaacagt tggctgtaat tttaagtgtg gtttctgcca
     1381 gaattttgag atatcacagg tctcttcaga ttatacaccc tatggtgcaa ccatatcgcc
     1441 tgctgaaatt gtaagatatg cgaaaaaata caattgtgac agtatttcat atacatacac
     1501 agaacccact gtttattttg aatttgcata cgagtgcgca gtgctggcaa aacaggcagg
     1561 actgaaaaat aattttgtga caaacgggta tatgagtgca gaggcgctta aaaaaattgc
     1621 gccatatctt gatgcggcaa atgttgacct taaaggagat gagaatttct ataaaaattt
     1681 atgtggagcc catctagcgc cagtactgaa aaacattcag gcgatgaagg aatatggaat
     1741 ctgggttgag g
//
LOCUS       DAORYU010000284         1745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_46108_length_1745_cov_3.871006, whole genome shotgun sequence.
ACCESSION   DAORYU010000284 DAORYU010000000
VERSION     DAORYU010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46108_length_1745_cov_3.871006"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1363)
                     /locus_tag="PLW07_07320"
     CDS             complement(<1..1363)
                     /locus_tag="PLW07_07320"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOL50378.1"
                     /translation="MEQKFVLAALMLSLMFVVSSLYSKEIIVVDRGKTEAKIVISWQA
                     SNFEKIAAEDLAKYIEKMTGARPEVISSQQEVETCLKRKDIPLFIIGSLAIKENPQLE
                     KKLDAVKKKNPVLRADAIVIKTEGNRIYLVGSNDESNCYAVGEILRRWGCRFFMPTEF
                     GQYVPNHSRLILDEIDYAYGSPFEVRGYWISWLGDTRGRDEFMRLNMMNNEVVPSGHV
                     LAKYTKDIAPGGDPFKIPISEKSTAEHVAAKIEKDYAADKRISLGMEDGLYSSVSPKD
                     KELMSLQWDKYFMTPSMTDAFMEFYNNVAEILMKKYPESKAKIGFLAYSNITLPPVRD
                     IVAKEPLVAYLAPIDIDPIHGMDDIESPPRQEYKEILYKWAKAMQGRVVIYDYDQGML
                     VWRDIPNPSIASIREDIKHYRNAGILGIYTESRGAFATTFINLYIRGRLMWNPDEDVD
                     KLIE"
BASE COUNT          519 a          376 c          274 g          576 t
ORIGIN      
        1 cttcaatcag tttgtcaacg tcttcatcag gattccacat caatcttccg cgaatgtaaa
       61 gattgataaa tgtcgtggca aaagcgcccc tgctttctgt gtaaatgcca agaattcccg
      121 cgtttctgta atgtttaata tcctctctga ttgaagcaat agaaggattt ggaatatctc
      181 tccatacaag cattccctgg tcataatcat aaatgactac tctaccctgc attgcctttg
      241 cccatttata aagtatctct ttatattcct gccttggcgg tgattcaata tcatccattc
      301 catgaattgg gtctatatca attggtgcaa gatacgcaac aagtggttct tttgccacaa
      361 tatctctcac agggggtaat gtgatattgg agtaagccag aaaaccaatt ttcgccttgc
      421 tttcaggata ttttttcatc aaaatttctg caacattatt gtaaaattcc ataaaagcat
      481 ctgtcattga aggggtcata aagtatttat cccactgaag ggacatcaat tccttatcct
      541 ttggagaaac tgaactatat agaccgtctt ccattcccag agaaatcctt ttgtctgctg
      601 catagtcctt ctcaatcttt gctgcgacat gttctgctgt tgacttttct gaaattggaa
      661 ttttaaatgg gtcaccacct ggtgcaatat cttttgtata tttggcaagt acatgcccgc
      721 ttggtacaac ctcattattc atcatattca accgcataaa ctcgtccctt cctcttgtat
      781 caccaagcca ggaaatccag tagcccctga cctcaaaggg cgaaccatag gcataatcaa
      841 tctcatcaag tataagccgt gaatgattcg gtacatattg cccgaattca gttggcataa
      901 aaaacctgca tccccacctt ctcaaaattt caccaactgc atagcagttt gactcatcat
      961 tgcttcctac aaggtaaatt ctgtttcctt ctgttttgat cacaattgcg tcagcgcgaa
     1021 gaacaggatt tttctttttt acagcatcaa gttttttctc caattgcggg ttttctttta
     1081 tagcaagtga gccaataata aaaaggggaa tatcttttct cttcaaacag gtttcaactt
     1141 cctgctgtga actgataact tcaggtcttg ctcctgtcat tttctcaata tatttcgcaa
     1201 ggtcttcagc agcaattttt tcaaaattgc tcgcctgcca agatattaca atttttgctt
     1261 ctgtttttcc tctgtcaaca acaataattt cctttgaata taaggaggaa acaacgaaca
     1321 tcaagctcag cattaatgcg gcaagaacaa atttttgctc cattaaaatc ctcctgtaaa
     1381 ttaggagtta ttatatcaca aatatatttt ttcaaggatg cctttttaat gcaaactgtt
     1441 ctcgaaaaat tggcaaagac cgattgcttg ttctatcccc atctcacata tataaacatt
     1501 tttcaatgcg tcctttttag caaacttatt tttctctact atcattccgc ctcatcgcaa
     1561 aaccactgac gaattgcttg gattagtttc tgtggtaaaa ttcttttaag tcaaatcaaa
     1621 aataaactat aaaaaacaaa atgatttgtg tatagaatac tgaaaaactt tttgcagagg
     1681 aaccacagaa gcaaaatggg agaaatatga ataaactttg tgaaatcaag ccatgcgata
     1741 ttgaa
//
LOCUS       DAORYU010000285         1737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_46409_length_1737_cov_4.538644, whole genome shotgun sequence.
ACCESSION   DAORYU010000285 DAORYU010000000
VERSION     DAORYU010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46409_length_1737_cov_4.538644"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1341
                     /locus_tag="PLW07_07325"
     CDS             <1..1341
                     /locus_tag="PLW07_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50379.1"
                     /translation="AESCCNWGPNGLGYWLAATLMWNAELDSNDLVDDFFDNCFKKAK
                     VPVRNIYERWAKAERFSPRTLKLALMDLNQAYSLEKDPAVQARLDRLAMYLCWLGMKM
                     DYERTVRHNLGDEKVKEAAKQLIIFSRRIMDTGLIHVYPMLFTHKTFEERLFPALMKI
                     KDIDKNEIEAWKKERQDIPQAEEVRQLFSEYVKKFEKLQAVEITGKIYSGNPVCLAEK
                     KPELLEKYKDVSISPLYLESGIYYFKAKKNEELNLTYKPFPNHTIDCKWTLYDIMGNL
                     VSQGEVKAEKNQEASIKLVIPEDGIYTFDPGTTYWKSAMITFDHRPLSVLAQRPDYPG
                     QKKSEPFRQWNPKGETLYFFVPAGTTHFVVGFPFLGKVYSKVDIKDSNGKVIFSDDKV
                     MNGDQISIIVPKGSDNQIWTIAITSLRNTVELYDLPPFLARHPAELLVPEDAIK"
     gene            1316..1729
                     /locus_tag="PLW07_07330"
     CDS             1316..1729
                     /locus_tag="PLW07_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50380.1"
                     /translation="MCLKTQSSNEKYIQFSPFSNTFFLFSSFIKPLFLFSPFVKLRQF
                     SPFVKLLLFSPFSKGGLRGISQAFSPFVKGRCKRDFINQPLFFLRQLSPFTKGGLRGI
                     SKGRRNLPQPLFYKEGSNVGELYKEGSRMGFCKGR"
BASE COUNT          564 a          339 c          380 g          454 t
ORIGIN      
        1 gcagaaagtt gctgcaactg gggtccgaat gggctcggtt actggcttgc tgcaacattg
       61 atgtggaacg cagaactgga tagcaatgac cttgttgatg atttttttga caattgtttc
      121 aaaaaggcaa aagtgcctgt gagaaacatt tatgaaagat gggcaaaagc agagcgtttt
      181 tcaccgcgca cattaaaact ggcgctaatg gatttgaacc aggcatattc cttagaaaaa
      241 gaccccgctg ttcaggcgag attggaccga cttgcaatgt atctttgctg gctcggtatg
      301 aagatggact acgaaagaac tgtgagacac aatctcggtg acgaaaaggt gaaagaagca
      361 gcaaaacagt tgattatctt ttcgcgtcgc atcatggata cagggttgat acatgtgtat
      421 ccgatgctat ttacacacaa aacctttgaa gaacgattat tcccggcact gatgaaaata
      481 aaggatattg ataaaaacga gattgaagcg tggaaaaagg agaggcagga tattccacag
      541 gcagaagaag tcagacaatt gttttctgaa tatgtaaaga agtttgaaaa actgcaggca
      601 gtcgaaatta cagggaaaat ttattcagga aatcctgtat gccttgcaga gaaaaagcca
      661 gaattattag aaaaatacaa agatgtcagt atcagcccgc tctatcttga atcagggatt
      721 tactatttca aagcaaagaa aaatgaagaa ttaaatctga cctataaacc attcccgaat
      781 cacaccatag attgtaaatg gacactgtat gatatcatgg gaaatcttgt ttcacaggga
      841 gaagtaaagg cagaaaaaaa tcaggaagca tcaatcaaac ttgtcattcc agaagacgga
      901 atatacacat ttgaccctgg tacaacatac tggaaatcag ccatgataac atttgaccat
      961 agacccctat cagtgctggc acaaaggcca gattatccag ggcagaaaaa gagtgaaccg
     1021 ttcagacagt ggaatccaaa aggagagaca ctgtactttt ttgtcccagc aggcacaaca
     1081 catttcgttg tgggatttcc atttctgggg aaagtttatt caaaggttga tatcaaagac
     1141 agcaatggga aagtaatatt ttctgatgat aaggtgatga acggagacca gatttccatt
     1201 attgtgccta agggctctga taaccagata tggacgattg ccatcacaag tttaagaaat
     1261 actgttgaac tttatgatct tccaccattt ctggcacgac atccagcaga actgcttgtg
     1321 cctgaagacg caatcaagta acgaaaaata cattcaattt tccccctttt caaacacctt
     1381 tttcctattc tcctccttta taaaacccct cttcctgttt tccccatttg taaaactccg
     1441 ccagttttcc ccctttgtaa aactcctcct gttttccccc ttttcaaaag ggggactaag
     1501 ggggatttct caggcatttt ctccctttgt aaaagggaga tgcaagaggg atttcataaa
     1561 tcagcccctc tttttcctcc gccagctttc cccctttaca aaagggggat taagggggat
     1621 ttcaaaagga aggagaaatc tcccccaacc cctcttttat aaagagggga gtaatgtggg
     1681 ggaactttat aaagagggga gtagaatggg gttttgtaag ggaaggtgac ctgaagt
//
LOCUS       DAORYU010000286         1727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_46801_length_1727_cov_3.547249, whole genome shotgun sequence.
ACCESSION   DAORYU010000286 DAORYU010000000
VERSION     DAORYU010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46801_length_1727_cov_3.547249"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="PLW07_07335"
     CDS             complement(<1..886)
                     /locus_tag="PLW07_07335"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOL50381.1"
                     /translation="MAEYFLEKERKVPVKGKYDVIVCGGGPSGFISAIASARAGAKTL
                     LIERYGFLGGTATAGMMVDFGSIYDGNQVIVGGITHEFLHSLEKYGGAWMRDEKTHSM
                     VFDPESMMMVSQEMVLEAGCTILFHSWICEPIVENNVVKGVITESKSGRQAFFGKVII
                     DTTGDGDIAARAGAQFEIGHGRPDILQPVTLEVIVGNVDVTKISVDAFMKVKTFIAEA
                     KQNNEWTIPTERFFSYGRVIKRGEPVNPKSSFFFFNATNVLNVNGCDVEDLTRAEIES
                     RRQVDCLIRFLRKYVPGFE"
     gene            1032..1328
                     /gene="rsfS"
                     /locus_tag="PLW07_07340"
     CDS             1032..1328
                     /gene="rsfS"
                     /locus_tag="PLW07_07340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOL50382.1"
                     /translation="MAILQEKKAENIVILDVRKLTWITDYLVITSGETTIQIRAMAEA
                     VIEKFNSPPFSIEGFETNWILLDYGDFVLHIFLPEFREFYNLEKLWLDAERVNL"
     gene            complement(1318..>1727)
                     /locus_tag="PLW07_07345"
     CDS             complement(1318..>1727)
                     /locus_tag="PLW07_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50383.1"
                     /translation="ITYYFTLNTIISEIQITSPAFDAVETVQKINLLLQKKLAGVFVA
                     LVVVAAILVIVYLHRVVGPVFRIERTLREVIEGKPFYPIKLRKKDSFKSLAEAINTFV
                     LIYEEKNQKAKEILDENLSDTEKIQQMKELFKD"
BASE COUNT          530 a          369 c          295 g          533 t
ORIGIN      
        1 tttcaaatcc tgggacatat ttacgaagaa aacgtattaa acaatcaacc tgtctacgtg
       61 attcgatctc agcccttgtg aggtcttcaa catcacagcc attcacattc aagacatttg
      121 ttgcattgaa aaagaagaat gaacttttcg ggtttactgg ctcaccccgt tttattaccc
      181 ttccataaga gaaaaacctt tcagtgggga ttgtccactc attgttttgc tttgcttcag
      241 caatgaatgt cttcactttc ataaaggcat caacagaaat ctttgtgacg tcaacattac
      301 caacaatcac ctcaagagtt acaggttgaa gaatatctgg ccttccatga ccgatttcaa
      361 attgggcacc tgctcgtgca gcaatatctc catcccctgt ggtatcaata atcaccttac
      421 caaaaaatgc ctgtcttcct gatttacttt cagtaatcac cccttttacc acattgtttt
      481 caacaatggg ttcacatatc catgaatgaa acagaattgt gcaacctgcc tcaagaacca
      541 tttcctgaga caccatcatc atactttcag ggtcaaaaac catgctatgg gttttttcat
      601 ctctcatcca ggcaccacca tatttttcga gcgagtgtaa aaattcatgg gtaatgccgc
      661 cgacaatgac ctggtttcca tcataaatgg aaccaaaatc taccatcatt cctgctgttg
      721 cagtacctcc aagaaagcca tatcgttcaa tcagtaaggt ttttgcaccg gctctcgctg
      781 aagcaattgc agagatgaac ccagagggac cgcctccaca aacaataaca tcgtatttcc
      841 cttttacagg aacctttctt tctttttcaa gaaaatattc cgccatgttt ctccccattt
      901 taataaaaac agaaccctta aatattaccc cctggtaatt ttttgtcaaa gatgcagaag
      961 ttgagaaaga ttggtaattc tggtataatt aggtaaaaag gagaaaacct atagaaaaac
     1021 tgaaaaaatt actggcaatt cttcaggaaa aaaaggcaga aaatatcgta atacttgatg
     1081 taagaaaact tacctggatt acagattatc ttgtcataac ttcgggcgaa acaaccattc
     1141 aaatcagggc aatggcagag gcagtaattg aaaagttcaa cagcccgcct ttttcaattg
     1201 aaggatttga aacaaactgg attttgcttg attacggcga ttttgtcctt cacatatttc
     1261 tgccagagtt cagggaattt tataatcttg aaaaactctg gcttgatgct gaaagggtca
     1321 atctttaaaa agttccttca tttgctggat tttttctgtg tctgacagat tttcatccag
     1381 aatttctttt gctttctgat ttttttcttc atagatcaaa acgaatgtgt taatggcttc
     1441 ggcaagtgat ttgaaactgt ctttttttct gagtttaata ggataaaaag gttttccttc
     1501 aatcacctcg cgcaaggttc tttctattct gaacactggt ccaacaaccc tgtgcaggta
     1561 tacaatcacc agaattgcag caacaacgac aagtgccaca aacacaccag caagtttttt
     1621 ctgaagtaat aggttgattt tctgtactgt ttcaactgcg tcaaatgcag gcgatgtaat
     1681 ttgaatttca ctgataatgg tattgagcgt gaaataatag gtaatac
//
LOCUS       DAORYU010000287         1726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_46857_length_1726_cov_4.665470, whole genome shotgun sequence.
ACCESSION   DAORYU010000287 DAORYU010000000
VERSION     DAORYU010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46857_length_1726_cov_4.665470"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..947)
                     /locus_tag="PLW07_07350"
     CDS             complement(138..947)
                     /locus_tag="PLW07_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009609817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOL50384.1"
                     /translation="MKIGVIPDCFRIPIKQAIKKAAELNLDGVQPYATRGDLAPENLT
                     KTGRDELKKFVSDLGLEISAIVGDFGGHGFTDSKTIDWRVGKTKEVIDLAVEIGVNIV
                     TTHIGIVPENPEAPEWKILLQSLPEVGNYASNKGVVLATETGPEPAEVLKKLLDQLNN
                     PGIRVNYDPANLVMVAGDNPVKGVHILKNYIVHTHAKDGIKLPDENGKKRWKELPLGE
                     GNVNFPEYLKAMKDIGYSGFFTIEREVGENPEADIVKARDFLRTLAEKLNY"
     gene            complement(944..1201)
                     /locus_tag="PLW07_07355"
     CDS             complement(944..1201)
                     /locus_tag="PLW07_07355"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOL50385.1"
                     /translation="MGKRFSVKINKNRCKGCGLCIEVCSQKNLIISKLFNKFGCHFVE
                     VIDNTNCNGCKKCAIICPDAAIEIFYEEETPKENTNNREDK"
     gene            complement(1180..>1726)
                     /locus_tag="PLW07_07360"
     CDS             complement(1180..>1726)
                     /locus_tag="PLW07_07360"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOL50386.1"
                     /translation="KDMEKVVQDVFSGHFRVSPRIYLEGHIVSPSNKATFVGRALNEM
                     VIFRKDIRMVEFELTLGSTVFYFKADGMIVSTPTGSTAHAFSAGGPIVFPEVRSLIIV
                     AFAPFTATWRNCIYEGGRVLLKPSRDCDIIIDGQEKINLPKEQLLEIQPGQESFKLMV
                     PENWDFWNTLKEKFSWGKGLV"
BASE COUNT          460 a          377 c          283 g          606 t
ORIGIN      
        1 catgaggaca gattgctccg actgcatgtt gtttatctcc ttcggcgaca atatactcat
       61 tcaaatccct gataagtttg cttttattac cagggtaaaa gtaaccactg acagatggtt
      121 tccttgtttt cttcatatta ataattcaac ttctctgcaa gcgttctaag aaaatccctt
      181 gctttaacaa tgtctgcttc aggattttca ccgacttctc tctcaatcgt aaaaaatcca
      241 ctgtatccga tatctttcat cgctttcaga tattcaggaa aatttacatt tccttctcca
      301 agcggaagtt ccttccatct cttttttcca ttttcatccg gcaattttat accatctttt
      361 gcatgcgtat gtacgatata attttttagg atatgtactc ctttgacagg attatcccct
      421 gcgaccatta ccagatttgc gggatcatag tttaccctga ttccaggatt gtttaattga
      481 tcaagcagtt tcttcaaaac ttctgcaggt tcaggccctg tttctgtggc aagaacaaca
      541 cctttatttg atgcatagtt tccaacttct ggtagagatt gcagaagaat tttccattca
      601 ggtgcttctg gattttcagg aacaattcct atgtgggtcg taacaatgtt aactccaatc
      661 tccacagcaa gatcaataac ttcttttgtt tttccaacac gccaatcaat cgtttttgag
      721 tcagtgaatc catgtccacc aaaatctccg acgattgctg atatttcaag accgaggtca
      781 gatacaaact ttttcaattc atctcttcct gttttcgtga gattttcagg tgcaagatct
      841 cctctggtag cataaggttg cacgccatca agatttaatt ctgctgcttt ttttatcgcc
      901 tgttttattg gtattctaaa acaatcagga ataactccga ttttcattta tcctccctgt
      961 tatttgtgtt ttctttgggt gtttcttctt cataaaagat ttcaatggct gcatccggac
     1021 agataatagc acatttctta cacccattgc agtttgtgtt gtcgattact tcaacgaaat
     1081 ggcagccgaa tttattgaat agttttgaaa ttatcaaatt tttttgggaa caaacttcaa
     1141 tacacagccc acaaccttta catctgtttt tattgatttt tacactaaac cttttcccca
     1201 tgagaatttt tcctttaatg tattccagaa atcccagttt tcagggacca ttaatttaaa
     1261 tgattcctga ccgggttgaa tttcaagtaa ttgttccttt ggtaggttta ttttttcctg
     1321 accatcaata ataatgtcac aatctcggga gggtttaagc aataccctgc ctccttcata
     1381 tatacagttt ctccatgtag cggtaaaggg cgcaaacgca acgattatta gtgatctgac
     1441 ttccgggaag acaatgggtc ctcctgctga gaaagcgtgt gcagtagatc ccgttggagt
     1501 acttactatc atcccgtctg ctttgaaata aaaaactgtg gaaccaaggg ttaattcaaa
     1561 ttcaaccatt cttatgtctt ttctgaatat taccatttcg ttcaaagccc ttccaacaaa
     1621 tgttgcctta tttgacggag aaacgatgtg cccttcaaga taaatacgtg gtgaaacgcg
     1681 aaaatgtcct gaaaacacgt cctgtaccac tttctccata tctttg
//
LOCUS       DAORYU010000288         1709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_47513_length_1709_cov_5.778114, whole genome shotgun sequence.
ACCESSION   DAORYU010000288 DAORYU010000000
VERSION     DAORYU010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47513_length_1709_cov_5.778114"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1184
                     /locus_tag="PLW07_07365"
     CDS             <1..1184
                     /locus_tag="PLW07_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50387.1"
                     /translation="DDCEQSFFNHIEMGLDYPDIASFLIAPRPMFIVANTHDIWDIEG
                     TEYVYNTAKRFYEMHGAADKLRISISDKGHSYDIKEQTDVLKWFNEIFDNKHDLVPFN
                     EITEKSFPSDKEIIVLPEKKERSFYLKNFLNVFTKNVQITKKDKSFIENIKNDLKKFA
                     QKTFTFEIIDRYPIGDIDCCRMVFYPEKNLVLPAEILIPRMPRGTIILLDEIKRTEKQ
                     HWQFELANKKNIVIRPDLRGLGETALKDDWPDIENWCQNNFSGKNFKLFILCHLLGRY
                     IVVERAKDILALVSIAAEQLKSKRITVHACGATALSAIFASIVDRRVQKLMLQDFLYS
                     FRDVFEKHYPVWKADHYIQGILKAGYDVEDLCSMSHARIEWKNGLDGIMRPVKNGKSS
                     N"
     gene            1165..>1709
                     /locus_tag="PLW07_07370"
     CDS             1165..>1709
                     /locus_tag="PLW07_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50388.1"
                     /translation="MEKVPIDDLIALIEKEYDEKRLQCAIERQIATQNNREPDYLPIF
                     LHGKVPEMEEFPSYDRKEQFYDPEKMLYMLLWGALAILRGKSDGIPCVRVNFGTGFLA
                     TVFGLQQEVFPDKMPWLKAHLDKKDILEMTVEDLEPIEEKGLMSEWKRYTEYYKEKLK
                     DIQFVKLYLPDTQGVFDLAHLV"
BASE COUNT          565 a          274 c          362 g          508 t
ORIGIN      
        1 aggatgattg tgaacagagt tttttcaatc atattgaaat gggtcttgat tatcctgata
       61 ttgcttcatt cttgattgcc ccaaggccca tgtttattgt tgccaatact catgacatct
      121 gggatataga aggaacagaa tatgtttaca ataccgccaa aagattttat gaaatgcacg
      181 gcgcagcaga taaattgaga atttcaatct ctgacaaagg gcattcttat gatatcaaag
      241 agcagacaga cgtgttgaag tggtttaatg agatttttga caacaaacac gatttggttc
      301 catttaatga gattacggaa aagtcgtttc catcagacaa agaaattatt gtgctgcctg
      361 agaaaaagga acgcagtttc tatctgaaaa attttctgaa tgtttttaca aaaaatgtgc
      421 aaataacaaa aaaagataag tctttcattg aaaacatcaa gaatgaccta aagaaatttg
      481 cccaaaaaac atttacattt gaaatcattg ataggtatcc tataggcgat attgattgtt
      541 gcaggatggt tttttatcct gaaaaaaatc ttgtgcttcc agcagaaatt cttattccca
      601 gaatgcctcg tggcacaata attttgcttg atgaaatcaa aagaactgaa aaacagcact
      661 ggcagtttga attagcaaac aaaaaaaaca ttgtgatcag accagacctg cgtggacttg
      721 gtgaaactgc cttaaaggac gactggcccg atattgaaaa ctggtgtcag aacaatttca
      781 gtgggaaaaa ttttaaactg tttattttat gccatcttct tggaagatac attgttgttg
      841 aaagggcaaa agatattctg gcacttgttt caatagcagc agagcagttg aagtcaaaaa
      901 ggattacagt ccatgcttgt ggtgcaactg cactatcagc aatttttgcc agtattgttg
      961 acaggcgagt tcaaaagctt atgcttcagg attttcttta ttccttcagg gatgtgtttg
     1021 aaaaacatta tcctgtctgg aaagcagacc attacattca gggaatattg aaggcaggtt
     1081 atgatgtgga ggatttgtgt tcaatgtcgc atgcccgcat tgaatggaaa aatggccttg
     1141 atggtataat gagaccagta aaaaatggaa aaagttccaa ttgacgacct gattgcgtta
     1201 attgaaaaag aatatgatga aaaacgtctg caatgtgcca tagaaaggca gattgctacc
     1261 cagaataatc gtgagcccga ctatcttcct atttttcttc atggcaaagt tcctgaaatg
     1321 gaagagtttc catcgtatga tagaaaagaa cagttttatg atcctgaaaa aatgctttat
     1381 atgcttttat ggggcgctct tgcaatactg cggggaaaat cagatggcat tccctgcgtc
     1441 agggtaaatt ttggaacagg tttccttgca acggtttttg gtttgcagca ggaagtgttt
     1501 cctgataaga tgccatggct taaagcccat cttgataaga aggatatcct tgaaatgaca
     1561 gttgaagacc ttgagccaat agaagaaaaa ggtttgatgt cagagtggaa aaggtacaca
     1621 gaatactata aggaaaaact taaggatatt cagtttgtaa aattgtatct tcctgataca
     1681 cagggagtgt ttgaccttgc tcatcttgt
//
LOCUS       DAORYU010000289         1708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_47571_length_1708_cov_3.344828, whole genome shotgun sequence.
ACCESSION   DAORYU010000289 DAORYU010000000
VERSION     DAORYU010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47571_length_1708_cov_3.344828"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1224)
                     /locus_tag="PLW07_07375"
     CDS             complement(<1..1224)
                     /locus_tag="PLW07_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOL50389.1"
                     /translation="MEKEKSGAKYDATKIQVLGGIEAVRKRPAMYIGDTSVFGLHHLV
                     YEVVDNSIDEAMAGYCTEISVVIHLDNSVSVADNGRGIPVDIHKTENKPAVEVVLTML
                     HAGGKFDRKAYRVSGGLHGVGVSVVNALSEWLEVEIRRDGKIYHQKYEKGNPVSQLTV
                     VGKTKDNGTTVTFKPDGEIFETVEFDDNILASRLRELAFLNPSLTIKFEDERKTRSEV
                     FKFDGGLVSFVEFLNKNKTALFSPPYCFRKEEGDMELDVAFQYNDGYNETILSFVNNV
                     NTRDGGTHLTGFRAGLTRGINEYGKKNQILDSDTTLSGEDVREGLTAVISLKIPDPQF
                     EGQTKTKLGNSTVKPFVESITFDKIYTFLEEHPDAARDILNKAITALKSREAARRARD
                     LTRKKLAEGGELPGKL"
     gene            complement(1342..>1708)
                     /locus_tag="PLW07_07380"
     CDS             complement(1342..>1708)
                     /locus_tag="PLW07_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50390.1"
                     /translation="GYTETSVLSYHCRKDFVLFGSLSRSGRYYDYVTDFRDFDGKNFL
                     IVSLNEKDIEKFVIYFQNVSVKIVEIMGAKFYLINGNHFIFNNYRDFYLRKISSMYYT
                     PVRFLPAGRNFFMERYFSR"
BASE COUNT          515 a          383 c          246 g          564 t
ORIGIN      
        1 aagtttgccg ggtagttccc ctccttcagc aagctttttc ctggtcaggt cccttgcccg
       61 tctggctgct tcccttgact tcaaggcagt gattgcttta ttaagaatat cccttgcagc
      121 atccgggtgt tcctcaagaa aagtgtaaat tttgtcaaag gtaatacttt ccacaaatgg
      181 tttcacagta gaattaccaa gtttcgtctt cgtttgacct tcaaattgtg ggtcaggaat
      241 ttttaagctg ataacagcag ttaaaccctc tctcacgtcc tcaccactga gagtggtatc
      301 tgagtcaaga atttgattct tcttaccata ctcattgata ccccttgtca aacccgctct
      361 gaaaccagtc agatgcgtcc caccatctcg tgtattaaca ttgttcacaa atgaaagaat
      421 tgtttcatta taaccatcat tatattgaaa cgccacatcc agctccatgt cgccttcttc
      481 ttttctaaaa caatacggag gagaaaacaa tgcagtttta ttcttattca gaaattccac
      541 aaatgacacc agtccaccat caaatttaaa aacctcactt cttgtttttc tttcatcttc
      601 aaatttgatt gtgagagatg gatttaaaaa agcaagttct ctcaatctgc tcgcaagaat
      661 attgtcatca aattcaactg tttcaaaaat ttcaccgtca ggtttgaatg taacagtcgt
      721 tccattatct tttgtttttc caacgactgt gagttgagaa accggattgc ctttttcata
      781 tttctggtga taaattttcc catctcttct tatttcaacc tcaagccatt ctgaaagtgc
      841 gttaactaca gaaactccaa caccatgtaa tccacctgaa accctgtatg ctttcctgtc
      901 aaattttcca ccagcatgaa gcattgtcag aaccacttct actgctggtt tgttttcggt
      961 tttgtgaata tcaacaggta tgcctcttcc gttatcagca acagatacac tattatcaag
     1021 atgtatcaca accgaaatct ctgtacaata acctgccatc gcctcgtcaa tactattatc
     1081 aacaacctca tatacaagat gatgcaaacc aaaaactgat gtatcaccta tgtacatcgc
     1141 gggtcttttt cttaccgcct caattcctcc aagtacctga attttcgtcg cgtcgtattt
     1201 tgctccagat ttttcttttt ccatcaagaa cctcctattt tttcaaattt aattttatca
     1261 ctttttttaa gtaaagtcaa ggaaaaattg catctctcat tttttgtaat atcaagaaaa
     1321 atcaattact taaagaggtg gtcaccttga aaaatatctt tccataaaaa agtttctgcc
     1381 ggcaggcaaa aatcggacag gagtataata cattgaagaa attttcctca aataaaaatc
     1441 gcgatagtta ttgaaaataa agtgattccc attgatcaaa taaaattttg ctcccataat
     1501 ttctacaatt tttactgata cgttctgaaa ataaataaca aacttttcaa tatctttttc
     1561 gttgagagat actatgagaa aatttttccc atcaaaatcc ctgaagtcag taacgtaatc
     1621 atagtatctt ccacttctcg acagtgagcc aaatagcaca aaatccttcc tgcaatgata
     1681 agatagcacc gatgtttcag tgtaacct
//
LOCUS       DAORYU010000290         1700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_47896_length_1700_cov_6.851064, whole genome shotgun sequence.
ACCESSION   DAORYU010000290 DAORYU010000000
VERSION     DAORYU010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47896_length_1700_cov_6.851064"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="PLW07_07385"
     CDS             complement(<1..566)
                     /locus_tag="PLW07_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007279715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOL50391.1"
                     /translation="MKDKKSGFTLVEVLVVIAIIGILAAILLPALAGARERARRTTCL
                     NNLKQIMAAFEMYADDYFENYPVADYSIYSGIYPNFIKTPQTFWCPSTISRGIRPPDK
                     IDDSNWNNSYAFVFGLTTGNNCPKPVPVISDKGIFAGSSADYGNHKFGMNVQYLDGST
                     SWLLQPKVVYYDKLHGKNGPIDGVNVACT"
     gene            complement(566..>1700)
                     /locus_tag="PLW07_07390"
     CDS             complement(566..>1700)
                     /locus_tag="PLW07_07390"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOL50392.1"
                     /translation="KQTSKPAAKKKAAGGISINLPFLGGTKIKQKELTVITRQLATLI
                     GSGLPLLRALKVLEQQRKGGAAKVLNKVASEIEQGALFSEALAKFPSSFPRIYVAMVK
                     AGEAGGALESVLSRLAEFMEKEAKLKGKIKSAMAYPSVVVVVMIGILTFIMVKIVPTF
                     TKIFEDMEVGDLPATTKLLIKMSKLMAERSYLIVAFIFGLIILYRVAVKIKFTKYLID
                     KVKLRMFIFGPVISKTIIARFARTFATLITSGVPILQSLQIVRDTVGNEVIANAINEI
                     RNRVREGEGISGIMLRTGAFPPMVTNMIAVGEETGAMDSMLEKVADAYEAEVDAAVAA
                     MTSMLEPILIVCLGVVVGFIVISLFMPLIKIALSLSGGGGGGE"
BASE COUNT          482 a          381 c          300 g          537 t
ORIGIN      
        1 gtgcaggcaa cattgacgcc atctattgga ccattcttcc catgtagctt atcataataa
       61 acaacctttg gttgaagaag ccaggaagtt gagccatcga gatactgaac attcattcca
      121 aacttatggt ttccataatc tgctgatgaa ccagcgaaaa ttcccttatc gctaatcact
      181 ggtactggtt ttggacaatt gtttcctgtt gttagtccaa aaacaaaggc gtatgagtta
      241 ttccagttcg aatcatcaat tttatctgga ggccttattc cccttgaaat tgtgctcgga
      301 caccagaatg tttgcggcgt tttgatgaaa tttgggtaaa tcccactgta aatactgtaa
      361 tctgccactg gatagttttc aaaataatca tcagcataca tttcaaatgc tgccattatt
      421 tgctttaaat tattcaggca ggtggttcgt cttgctcttt cccttgctcc agcaagggct
      481 ggaagtaata ttgctgcaag aattcctatg attgcaataa caacaagaac ttcaacaagg
      541 gtaaagccag attttttatc tttcattatt caccaccacc tcctcctcct gataaactga
      601 gcgcgatttt tatcagaggc atgaaaagag aaataacaat gaaaccaacg acaactccaa
      661 gacaaacaat aagaattggc tcaagcatgg atgtcatcgc tgcgacagca gcgtcaactt
      721 ctgcttcata agcgtcagca accttttcaa gcatagaatc cattgcacct gtttcttctc
      781 caaccgctat catatttgtg accattggag gaaatgcacc tgttctcaac ataattcctg
      841 atataccttc tccttcgcgc acccggtttc taatttcgtt gatggcattc gcaatgactt
      901 catttccaac agtatctctg acaatttgaa gggattgaag tattggcaca ccgcttgtaa
      961 tcaatgtggc aaatgttctg gcaaacctcg caataatcgt ttttgaaatc actggtccaa
     1021 aaataaacat tctgagtttt accttatcaa tgagatactt tgtaaattta attttgactg
     1081 ccaccctata aaggattatt agaccaaaaa tgaatgccac aatcagatat gaacgttctg
     1141 ccattagttt tgacatctta atcaaaagct ttgttgttgc tggaagatct ccaacttcca
     1201 tatcctcaaa aatctttgta aatgttggca caattttaac cataatgaaa gtcaaaattc
     1261 caatcataac cactacaaca acacttggat atgccatggc tgacttgatt tttcctttca
     1321 gttttgcctc tttttccata aattctgcaa gacggcttaa cactgattca agtgcgccgc
     1381 ctgcctctcc tgcttttacc atggcaacat aaattctcgg gaaacttgaa gggaattttg
     1441 caagggcttc tgaaaaaagc gcgccctgtt caatttcact cgccaccttg ttaagaacct
     1501 ttgcagcacc accttttctc tgttgctcaa gaaccttcaa tgctcgcagt agaggtaggc
     1561 ctgaaccaat gagtgtagct agctgtcgtg taataacagt gagttctttc tgcttgattt
     1621 ttgttccgcc aagaaacggt agattgatac ttataccgcc ggctgctttt ttctttgctg
     1681 ctggttttga tgtttgtttt
//
LOCUS       DAORYU010000291         1672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_49064_length_1672_cov_3.044527, whole genome shotgun sequence.
ACCESSION   DAORYU010000291 DAORYU010000000
VERSION     DAORYU010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49064_length_1672_cov_3.044527"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1672)
                     /locus_tag="PLW07_07395"
     CDS             complement(<1..>1672)
                     /locus_tag="PLW07_07395"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOL50393.1"
                     /translation="YLEILGYRWFFPGENWTIVPSLKSITVHINKIESPVFKQRDFFG
                     TGGFGGKLPVDPEMKLSKKWDEWKKRNFLGGEIRISGHTGEAFNAANKAILLEHPEYL
                     AEIGGKRQPWSPGTKVCYSNPGLIELYISDRLKQLKRQIDAAPDGPGSFAVSVEPSDG
                     GGHCECAECQKIGTVSDRVFFLANRVAKEVAKQYPGKYVSLLAYNEHAAVPTIDLEPN
                     VYVVVVPYGFQRTGYSGDELIQMWAKKTKFFGVYDYWSIPDWTHDMPSLNYLEIPGEK
                     IRFWHNNHVAAFLSESTYSSGAVGITWYLSSRMLWNPDVDEKQLLDDFYKKAFGEAEK
                     PMRRMLERWAKSFMLTDHELALSFRDISEADKLARDPLVRARIDDYKKYLIYLSLFYD
                     YTSAKPGSPERKQAVENLLNFMWRIYDSTMVHVYRMHQLIINRYESNPELRQLWDFKN
                     KEKWERIKPVTSEELDELVKIGLEKYKLYDFEPKQYDITRLVPVKDVITVTPDAKYIS
                     TNSFHGNHTFAFYVPEGVQNLTLRMTVGKRENHPGDRCVIKDDTKKVVF"
BASE COUNT          456 a          383 c          284 g          549 t
ORIGIN      
        1 caaaaacaac tttttttgta tcatccttga tcacacatct atctccagga tgattttctc
       61 ttttgcccac tgtcattctg agtgttaaat tttgtacacc ttctggtaca taaaaggcaa
      121 acgtatgatt tccgtggaaa gaattcgttg aaatatattt tgcgtcagga gtaaccgtaa
      181 ttacatcttt tacagggaca agccgcgtta tatcatattg ttttggttca aagtcgtata
      241 atttatactt ttcaagacca attttcacca gttcatcaag ttcttcagaa gtaacaggtt
      301 tgatcctttc ccatttttct ttatttttaa agtcccacaa ctgcctcaat tcagggttgg
      361 actcgtatct gttgataatc aattgatgca ttctgtaaac atgaaccatt gttgaatcat
      421 aaattctcca catgaaattc aacaaatttt ccactgcctg ttttctttct ggagacccgg
      481 gttttgctga ggtgtaatca taaaaaaggg aaaggtagat caggtatttt ttgtaatcat
      541 caattcttgc tctcactagc gggtctctgg caagtttgtc ggcttctgaa atatccctga
      601 aagaaagcgc aagttcgtgg tcagtcaaca taaaggattt cgcccacctt tcgagcattc
      661 ttctcatggg tttttctgct tcaccaaatg cctttttata aaaatcatca aggagttgct
      721 tttcatctac atcaggattc cacagcattc ttgaagaaag ataccaggta attcccacag
      781 cgccactact ataagtgctt tcagaaagaa atgctgcaac atgattgttg tgccagaacc
      841 ttattttttc ccctggtatc tcaagataat tcagggacgg catgtcatgt gtccagtcag
      901 ggatagacca gtaatcatat acaccaaaaa actttgtttt tttcgcccac atctggatga
      961 gttcatcacc actatatcct gttctctgaa atccgtaagg taccaccaca acatatacat
     1021 ttggctcaag gtcaatggtt ggaactgcag catgttcatt gtaggcaagc aaacttacat
     1081 atttaccagg atactgcttt gccacttctt ttgccacccg gtttgccagg aaaaatactc
     1141 tatcactgac agtacctatt ttttgacatt ctgcgcactc gcaatgtccc cctccatctg
     1201 acggctctac gctgacagca aaagagcctg gtccatcagg ggctgcatca atctggcgtt
     1261 tcaattgttt caatctatcg ctgatataaa gttctatcaa acccggattt gaataacaca
     1321 cttttgttcc aggactccac ggttgccttt ttccacctat ttctgcaaga tattcaggat
     1381 gttccagaag tattgctttg tttgccgcat tgaatgcctc accagtatgt cctgaaatcc
     1441 tgatttcgcc accgagaaag tttctttttt tccattcatc ccacttcttt gataatttca
     1501 tctctgggtc aacaggcagt tttccaccaa acccaccagt tccaaaaaaa tccctttgct
     1561 taaacacagg gctttcaatt ttgttgatat gcaccgtgat acttttcaat gatggtacaa
     1621 ttgtccagtt ttcaccaggg aaaaaccatc tatatccgag aatctcaagg ta
//
LOCUS       DAORYU010000292         1671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_49093_length_1671_cov_4.517946, whole genome shotgun sequence.
ACCESSION   DAORYU010000292 DAORYU010000000
VERSION     DAORYU010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49093_length_1671_cov_4.517946"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="PLW07_07400"
     CDS             complement(<1..338)
                     /locus_tag="PLW07_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50394.1"
                     /translation="MKEKFVYDRSQLPEPVYEEKPEFIDIYHACWQTAFQNIEYPEKR
                     NWLPYITCMPGSGNTWQWDSCFMVFFLRYSNGTIPATNNLDNLYQLQRKDGYISMAYQ
                     IKTEKPAYGE"
     gene            complement(408..1250)
                     /gene="rfbD"
                     /locus_tag="PLW07_07405"
     CDS             complement(408..1250)
                     /gene="rfbD"
                     /locus_tag="PLW07_07405"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOL50395.1"
                     /translation="MKILITGGTGLVGRYLSKIVQAERPVVLSRKDYDITDFFSVRTL
                     FYKIKPDVVFHLAAFTNVDLSEQDPISAFRINVNGTNNIVFFAEKTDAHIIYLSTDFV
                     FDGMKTQPYEEQDIPNPLSIYGKTKFEGEKIVSRNCSKYSIVRTSRIFGKNGSNFAST
                     LPDKLLRGEKTMVTTDLINSPTYAKDLAMALSEIAKKQFYGIIHFCNRGFCNWYEYAL
                     YICKHLSINTSLLTPLSIKNFHQSIAERPQFSALDTTLFSKNFYHPRTWQEALEKYLE
                     YEIS"
     gene            complement(1247..>1671)
                     /locus_tag="PLW07_07410"
     CDS             complement(1247..>1671)
                     /locus_tag="PLW07_07410"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOL50396.1"
                     /translation="NALENKKIPLYGDGQQIRDWLYVFDTCRAVDLLIEKGEPGQIYN
                     VSAHQERKNIDVLSEILKILGKDKDLMVNIPDRIAHDRRYSLSSEKIQQLGFLPVYDF
                     EKGLEETIKWYLDNKKWWQFIKNSSEFQSYYKKRYPAL"
BASE COUNT          474 a          308 c          293 g          596 t
ORIGIN      
        1 ctctctccat acgcaggttt ttctgttttt atttgatatg ccatgctgat atacccatct
       61 tttctctgaa gttgatacag attgtcaagg ttatttgttg ctggaatcgt tccatttgaa
      121 tatcttaaga aaaacaccat aaaacatgaa tcccattgcc atgtgtttcc tgaacccggc
      181 atgcaggtga tgtagggaag ccagtttctt ttttcaggat actcaatgtt ctgaaaagca
      241 gtttgccagc acgcatgata gatatcgata aattcaggtt tttcttcata cacaggttct
      301 ggcaactgac ttctgtcata aacaaacttt tctttcattc ttatactcct gttagttttg
      361 aataatcctg tcgttgtttc agatttccta tgctattttt atctccttca agaaatctcg
      421 tattccaggt atttttcaag agcctcctgc caggttcttg gatgatagaa atttttactg
      481 aacagtgttg tgtcaagggc agaaaattga ggcctttctg ctattgattg atgaaaattt
      541 ttaatactca aaggggttaa taaggacgtg tttatactta aatgtttaca tatgtataac
      601 gcatattcat accagttaca aaaaccccta ttacagaaat ggattattcc ataaaattgc
      661 tttttggcta tttcagacaa tgccatagca aggtcctttg catatgtcgg ggaatttata
      721 aggtccgttg tgaccatggt tttttcaccg cgaagcaatt tgtctgggag agtacttgca
      781 aaattactac catttttacc gaatatcctc gacgtacgta caatgctgta ttttgaacag
      841 ttgcgtgata caattttttc tccttcaaat tttgtttttc cataaattga aagtgggttc
      901 gggatatcct gttcttcata gggttgtgtt ttcattccat caaaaacaaa atctgtgctg
      961 agataaataa tgtgtgcatc tgttttttct gcaaaaaaaa caatgttatt ggtgccatta
     1021 acattgattc taaaagccga aatggggtcc tgttcagaaa ggtcaacatt ggtaaatgcg
     1081 gcaagatgaa atacaacatc aggctttatc ttataaaaca gggttctgac ggagaagaaa
     1141 tctgtaatat cataatcttt tcttgataga acaaccggtc tttcggcttg aacaattttt
     1201 gatagatacc ttccaacaag gccggttcca ccggtaatca gaattttcat agagcagggt
     1261 atcgtttttt ataataactt tgaaactcag aactgttttt tataaactgc caccattttt
     1321 tattatcaag gtaccattta atcgtttctt caagaccttt ttcaaaatca taaacaggca
     1381 aaaatccaag ttgttgaatt ttttctgatg ataaactgta tcttctgtcg tgagctatac
     1441 ggtcggggat atttaccata agatctttgt cctttccaag aattttcaag atttcagaga
     1501 gcacatcaat attttttctt tcctggtggg ctgaaacatt gtaaatttgg cctggttctc
     1561 ctttttcaat gagcagatca accgccctgc atgtatcaaa cacatatagc cagtcccgta
     1621 tttgttgtcc atctccatac aatggaattt ttttgttttc aagagcattt g
//
LOCUS       DAORYU010000293         1671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_49105_length_1671_cov_2.845297, whole genome shotgun sequence.
ACCESSION   DAORYU010000293 DAORYU010000000
VERSION     DAORYU010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49105_length_1671_cov_2.845297"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..1343)
                     /gene="fabF"
                     /locus_tag="PLW07_07415"
     CDS             complement(96..1343)
                     /gene="fabF"
                     /locus_tag="PLW07_07415"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006676626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOL50397.1"
                     /translation="MKEKKEVVITGMGLVTPVGNNVSAVWKNLVEGKSGIGLITDFDA
                     SEFPTKIAAEVKDFNLDVIDKKTARRMDRFVQFAVTAAHEAINDSGLKLDNENKDKIG
                     VIIGAGIGGLRIIEQQHSQLLNSGPSRVSPLLIPMLIPDMAAGQVAITFGLRGPNFAT
                     VSACASGAHAIAVACELIRTGKTDIVIAGGTESCITPLGVAGFCSMKALSERNDDPER
                     ASRPFDAERDGFVMGEGSGIVVMESLEHAKARGSRIYAKMPGYGMSCDAHHITAPDPS
                     GTGPYLCMKYALEDAGIEPQQVTYINAHGTSTPLNDKSETLAIKKALGEHAYKIPVSS
                     NKSMIGHLLGAAGAVEFINTILTIYHGIIPPTINYQTPDPECDLDYVPNSARKADVEI
                     ALSNSFGFGGHNICLCVQKYKGD"
     gene            complement(1340..1588)
                     /locus_tag="PLW07_07420"
     CDS             complement(1340..1588)
                     /locus_tag="PLW07_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013012247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOL50398.1"
                     /translation="MERSEIEKKVKEIVMEKLQVEAGAVIDNASFVDDLGADSLDTVE
                     LVMDFEEKFGVEIPDEDAEKIRTVGDAIKYLEEKTKTQ"
BASE COUNT          442 a          425 c          271 g          533 t
ORIGIN      
        1 atagtatgct cgttttggtt ttattttttc ttctgcaact tcacgagcaa gcgctttgat
       61 cattttctgt tcatcagtca ataaatagtc cattttcaat cccctttgta tttctgcaca
      121 caaagacaga tattatgacc tccaaaaccg aatgaatttg ataacgcaat ttccacatct
      181 gcttttctcg ctgaatttgg aacatagtca aggtcacatt caggatccgg tgtctggtag
      241 tttatggttg gcggaataat accatgatag attgtcagta tcgtattgat aaattccaca
      301 gcccctgctg cgccgagaag atgtccaatc attgacttat tggaactgac tggaatcttg
      361 taagcatgct cacccagggc tttttttatc gcaagtgttt cacttttatc gttgaggggc
      421 gttgatgttc catgagcatt aatataagtc acctgctgtg gctctatacc tgcatcttca
      481 agtgcatatt tcatacagag atacggaccg gtgccactcg ggtctggcgc cgtaatatga
      541 tgtgcatcac agctcatccc gtatccaggc attttcgcat aaatacgact gcctctcgcc
      601 tttgcgtgtt ctaacgattc catcacaaca attccacttc cctctcccat cacaaaaccg
      661 tctctttctg catcaaacgg tctacttgcc ctttcagggt catcatttct ttcagagaga
      721 gccttcatgg agcaaaagcc agcgacgccc aaaggtgtaa tacatgactc tgtccctcct
      781 gcaataacaa tatctgtctt acccgtacga atcaattcac acgcaaccgc tattgcatgt
      841 gctccagaag cacaggcgct cacagtagca aaatttggac ctcttaaacc aaaagttatt
      901 gccacctgac ctgcagccat atctggaatg agcatgggaa tcaacaaagg cgacactctt
      961 gaaggaccgg aattcaacag ttgtgaatgt tgttgttcaa taatcctcaa tcctccaatg
     1021 cctgctccaa taatgacacc tattttatcc ttgttttcgt tgtccagttt gagcccgcta
     1081 tcattgatcg cttcatgggc tgctgtaact gcgaactgaa caaaacgatc cattctccgt
     1141 gcagttttct tatcaattac atcaagatta aaatccttca cttctgcggc aatctttgtt
     1201 ggaaattcag acgcatcaaa atctgtaatg agccctatac cacttttacc ttcgacaaga
     1261 tttttccaca cagcagaaac attatttcca acaggagtaa ccaggcccat tcctgttatt
     1321 acaacttctt ttttttcttt cattgggtct ttgttttttc ctcaagatat ttgatcgcat
     1381 cacctactgt tctaattttt tctgcgtctt catctggaat ttccaccccg aatttctctt
     1441 caaaatccat aacaagttca actgtatcaa gagaatctgc accaagatca tcaacaaatg
     1501 atgcattatc aatcactgca cctgcctcca cctgcaattt ttccatgaca atttctttta
     1561 cttttttttc aatttcactt ctttccattc aacctcccat gttttcatgt aaaattttca
     1621 cattaccaat ccaccatcaa cccgaaacac ctggcctgtg atataggaag c
//
LOCUS       DAORYU010000294         1668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_49228_length_1668_cov_3.536268, whole genome shotgun sequence.
ACCESSION   DAORYU010000294 DAORYU010000000
VERSION     DAORYU010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49228_length_1668_cov_3.536268"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="PLW07_07425"
     CDS             complement(<1..424)
                     /locus_tag="PLW07_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50399.1"
                     /translation="MRNPQTILLAFILCLGLTGCTTVAMKDDVSILKEEIEQKLAANE
                     TTSSNRFQEVNTRIEQLQQTQEQQQASLLKLAEGLKTQVNDLKIAFDDSAQNQGKEFE
                     LFKKNQNEKNNQFTQDIETLRKSQNDLIKTSVSLTDSMV"
     gene            complement(421..1044)
                     /gene="pal"
                     /locus_tag="PLW07_07430"
     CDS             complement(421..1044)
                     /gene="pal"
                     /locus_tag="PLW07_07430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HOL50400.1"
                     /translation="MKSAIKLLMCISVLMFIFSGCCPYSKKPVPQSKVEPTTSEQPSV
                     TQQPTVTPPTVTTTQTSGTATSDERLIASTGGEIPPVKPPETPIFVEPGEQLKSVFQP
                     IYFDFDRYNIKTSEQGKLQVIANYLKKFPSVSILIEGHCDERGTDEYNLVLGEQRALS
                     TRNFLIGLGISPNRLYTISYGEAKPADPGHNEEAWSKNRRCEFKIKQ"
     gene            complement(1088..>1668)
                     /locus_tag="PLW07_07435"
     CDS             complement(1088..>1668)
                     /locus_tag="PLW07_07435"
                     /inference="COORDINATES: protein motif:HMM:NF022113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LpqB family beta-propeller domain-containing
                     protein"
                     /protein_id="HOL50401.1"
                     /translation="IETFLAEPGLNACASFFRNQNRAAVVLSRSGNPDIYLVDLKGTI
                     LKRLTEKKGLNASPSVSPDGENIAFVSDRDGKTRLWVMNTYGLNARKLSIPSNYITSP
                     CWSPDGKHLAYAARYGTEMFIEICEWETGKRKILTQSLSWCDAPSWAPDSRHIIFTRQ
                     DKFVNSLWTIDIYSLKMRKIADNAWSSCWDIR"
BASE COUNT          413 a          327 c          337 g          591 t
ORIGIN      
        1 tgaccattga atctgtcaga gaaacagagg tttttatcag atcgttttga gattttctta
       61 acgtttcaat atcctgtgta aactggttat ttttttcgtt ctgatttttt ttgaaaagtt
      121 caaattcttt tccctgattt tgagcagagt cgtcaaaggc aatttttaga tcgttaactt
      181 gagttttcaa accctctgcc aatttgagca aagaggcctg ttgctgttcc tgggtctgct
      241 gtaactgctc tatcctggta ttaacttcct gaaatctgtt tgagctggtt gtttcgttcg
      301 ctgccaattt ttgttctatt tcttctttaa gaatactgac atcgtctttc atggcaacgg
      361 ttgtgcaacc agttaaacca agacacaaaa taaaagccag tagaatagtt tgcggatttc
      421 tcattgtttt attttaaatt cacaccttct gttctttgac catgcttctt cgttgtgacc
      481 tgggtctgct ggttttgctt caccatagct aatggtataa agtctgtttg gagagatacc
      541 aagtcctatc aggaaatttc tcgtactcag cgccctttgt tcgccaagga caagattata
      601 ctcgtctgtt ccgcgttcgt cacaatgacc ttcaataaga atagaaaccg atggaaattt
      661 cttcagataa ttagcaatga cttgcaattt cccctgttca gaagttttga tgttgtacct
      721 gtcaaagtca aaatagattg gctggaagac ggatttcagc tgttcaccag gttcaacaaa
      781 aattggtgtt tctggtggtt ttacgggtgg tatttctccg cctgttgaag cgattaatct
      841 ttcatctgat gtggctgtac ctgatgtttg tgtggttgtt acagtaggtg gtgtgacggt
      901 cggttgttgt gttactgatg gttgttcaga tgttgttggt tctacttttg actgtggtac
      961 aggttttttt gaataaggac agcaacctga aaaaatgaac attaacactg aaatgcacat
     1021 taacaatttt attgctgatt tcatttttcc cccctttaag ttgattatat caaagtcctg
     1081 ttatttgtca acgaatgtcc cagcaactac tccatgcgtt gtccgcgatt tttctcatct
     1141 ttaaggaata tatatctatt gtccataagg aattgacaaa tttatcctgc cgggtgaaga
     1201 ttatatgtct gctatctggt gcccatgaag gtgcatcaca ccaggatagt gattgtgtga
     1261 gaatttttct tttaccagtt tcccactcgc atatctcaat gaacatttct gtgccatatc
     1321 tcgctgcata cgcaagatgt tttccgtctg gtgaccagca gggagatgtg atgtaatttg
     1381 acggaatact tagttttctg gcattcaatc cataagtatt catcacccac agccttgttt
     1441 tgccatccct gtcagacaca aacgcaatgt tttctccgtc aggagaaact gacggagagg
     1501 cgttcaatcc ttttttttca gtaagtcgtt tcaaaatagt tcccttcagg tcaacaagat
     1561 aaatatcagg gtttccgctt ctgctcagaa cgactgcagc gcgattttga tttctaaaaa
     1621 acgatgcgca tgcattgagt cctggttctg cgaggaatgt ttcaatct
//
LOCUS       DAORYU010000295         1666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_49306_length_1666_cov_3.780261, whole genome shotgun sequence.
ACCESSION   DAORYU010000295 DAORYU010000000
VERSION     DAORYU010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49306_length_1666_cov_3.780261"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..1029
                     /locus_tag="PLW07_07440"
     CDS             217..1029
                     /locus_tag="PLW07_07440"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOL50402.1"
                     /translation="MKISVYSLSASDKKVQEVIELAKQYQCDGIEWWCRENAHIDLTD
                     LKNSAINVSKMMDGSGLECAGLAPYFKFNEPADYLSKIFEAACILKTRNVRCHSYIFD
                     GNVPVEELMKTQRSWLEKVVMPEAERFDIRFNIEQHHYNICCTPNACRQLVDGLPERH
                     FGIIFDPGNSAAEGFTRPEYSISVFGRYLAHVHAKNCKQTEIREGAILGRKYKMEFGS
                     LEEGDIDWVAIIKGLHKADFQGFVSLEALDNRPSELKFQQDIVFLHNALKQI"
     gene            1042..>1666
                     /locus_tag="PLW07_07445"
     CDS             1042..>1666
                     /locus_tag="PLW07_07445"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOL50403.1"
                     /translation="MDKTLHGLIPAIVIPFDEYMNIERDILAQYIERYIVQAKIVAIA
                     VNTDAGEGAFLTQQEKEEILSITKKHSKNMPVVCGLGAPTTQQAVEYGKRYKDLGANY
                     FLVFPHFAFRGSKGKDKAVIEYHYALSKIGVPMILFQLQEDLGGVSYSEETIQELVQI
                     DNVVAIKEASFDALKFRNMHALLHSLPKRISLLTGNDNFIIESFILGA"
BASE COUNT          551 a          292 c          351 g          472 t
ORIGIN      
        1 agaaaatcat gaaaatactc aacaatcgcg atttgaaacc ataccttgat gccatcatca
       61 caaagaaaat cactcaaact gccctttaca gatatgcatc tgactggacg gattttcaat
      121 ttcgtctcaa ggaaaatttt gttggcacaa tacaggaatt gtttctgtga aatgtgttgt
      181 gtataataaa cccaaatttc taacaaggag aaaatgatga aaatttctgt gtattctctt
      241 tctgcgtctg ataaaaaagt gcaggaagtc attgaacttg caaaacagta tcagtgtgat
      301 ggcattgaat ggtggtgcag ggaaaatgcc catatagacc tgacagattt gaaaaattct
      361 gccataaatg tttcaaaaat gatggatggc tcaggtcttg agtgcgcggg acttgctcct
      421 tattttaagt tcaatgagcc tgctgattat ttaagcaaaa tttttgaggc agcatgtatc
      481 cttaagacaa gaaatgtgag atgccacagt tacatatttg acgggaatgt tcctgttgaa
      541 gagttaatga aaacacagag gtcatggctt gaaaaggttg taatgccaga agcagagagg
      601 tttgatatca ggttcaatat tgagcaacat cattataata tctgctgtac ccccaatgca
      661 tgcaggcaac ttgttgatgg cttaccagaa agacactttg gaattatttt tgaccctggt
      721 aactctgcgg ctgaaggttt tacaagacct gaatactcca tcagcgtttt cggaagatat
      781 ctggcgcatg ttcatgcaaa aaactgtaaa cagacagaaa ttcgcgaggg cgcaatttta
      841 ggacgcaaat acaaaatgga atttggttct ctcgaagaag gagatattga ctgggttgcc
      901 attattaagg gacttcataa agcagatttt cagggatttg tttcccttga agcccttgat
      961 aacagacctt cagaattaaa gttccagcag gacatcgtgt ttctgcacaa cgcactgaag
     1021 caaatctgaa ttggaggtag catggataaa acattacatg gattgattcc agcaattgtg
     1081 attccgtttg atgaatatat gaacattgaa cgtgatatcc ttgcgcaata tattgagcgg
     1141 tatattgttc aggcaaaaat agtagcaatc gcagtgaata cagatgcagg agaaggtgca
     1201 ttccttaccc agcaagaaaa agaagaaatc ctgtcaatca caaagaaaca ttctaaaaat
     1261 atgcctgttg tgtgcggact tggtgcacca acaacacagc aggcagttga atatggaaaa
     1321 agatataagg acctgggtgc taattatttc ctcgtatttc cgcattttgc attcaggggc
     1381 tcaaaaggaa aagacaaagc agtcattgaa taccactatg ccctttcaaa aataggggta
     1441 ccaatgatat tatttcaatt gcaggaagat ctgggtggtg taagttattc tgaagaaacc
     1501 attcaagaac ttgtccagat tgacaatgtt gtggcaatta aagaagccag ttttgatgca
     1561 ttaaaattta gaaacatgca cgcacttcta cattcacttc caaaaagaat ctcacttctg
     1621 acaggaaatg ataacttcat tattgaatcg tttatccttg gtgctg
//
LOCUS       DAORYU010000296         1655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_49774_length_1655_cov_2.848125, whole genome shotgun sequence.
ACCESSION   DAORYU010000296 DAORYU010000000
VERSION     DAORYU010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49774_length_1655_cov_2.848125"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="PLW07_07450"
     CDS             complement(<1..893)
                     /locus_tag="PLW07_07450"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004083005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOL50404.1"
                     /translation="MSLQRKKMGILVGGGPAPGINGVIGSATIEAINNGIEVIGIYDG
                     FKWLSKGDITHTKKLSISDVIKTRFDGGSILNTSRENPTKSEEKMKNVVNSLKKLGID
                     CLVTIGGDDTAFSASQTYKFAKGSIRVAHVPKTIDNDLPLPANMPTFGFQTARHVGAE
                     LVANLLEDARTTHRWYLVVAMGRTAGHLALGMGIGGGATVTIIPEEFKKTPTIEDVCN
                     IIEGSIYKARALGQDYGVAVIAEGVGDLLKNELKDNPLAIVEHDEHGHFRMAEVPLCL
                     IIKRTLNQRFQDRGEKAQFIDV"
     gene            complement(945..>1655)
                     /locus_tag="PLW07_07455"
     CDS             complement(945..>1655)
                     /locus_tag="PLW07_07455"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOL50405.1"
                     /translation="MFLRQCHGIKIKGVTEKNYNGDGISFQQCTNLIIEDCDISKNYG
                     SGLHPGSGSAGVVMRNCKITGNRKDGVFYCLRVTYTLLENCEISENGNDGISVGARDT
                     DHIIRNNLITKNFRHGIYFRKADKTMGGHRNLIDSNMIVNNCQKEGNTEVFIEDVTEN
                     IWFKNNKIQGEKKSSYGIISGKNCKKICLGNDNKFIGLKHDIKPGKVFLYTLPEKILV
                     GPDYLDNKSVRHLNIELH"
BASE COUNT          465 a          374 c          246 g          570 t
ORIGIN      
        1 acatcaataa actgtgcttt ttcgccccta tcctgaaatc tttggtttag cgttctcttg
       61 ataatcaagc ataaaggcac ttcagccatc ctgaaatgac catgttcatc atgctcaaca
      121 attgcaagag ggttatcctt taattcattc tttaaaagat ctccaacacc ttctgctatt
      181 acggctacac cgtagtcctg tcccaacgcc cttgctttgt agatggaacc ttcaataatg
      241 ttgcagacat cttcgatggt tggcgttttt ttaaactctt cgggaatgat agtaactgtg
      301 gctccaccac caatacccat accaagtgca aggtgccctg ccgttctgcc cattgcaacc
      361 accagatacc atctgtgtgt ggttcttgcg tcttcaagaa gatttgcaac aagttctgcg
      421 ccgacgtgtc ttgctgtctg aaaaccaaac gtgggcatat ttgctggaag tggcaaatcg
      481 ttatcaatag ttttcggaac atgtgctacc cgaatacttc cttttgcaaa tttatatgtt
      541 tgactcgcgc taaatgcagt atcatctcca ccaatcgtaa caaggcaatc gatgccaagt
      601 ttttttaagg aattcactac atttttcatt ttttcttcgc ttttcgttgg attctctcgc
      661 gaggtattca aaattgaccc accatcaaaa cgagttttta tgacatcgct gattgagagt
      721 ttctttgtat gcgtgatgtc tccctttgaa agccatttga aaccatcata aataccaatg
      781 acttcaatac cgttgttaat tgcttctatt gttgcagaac caataacacc attaattccc
      841 ggtgcaggcc ctccaccaac aagaattccc attttctttc tctgtaaact cattgctcct
      901 cctggtcaag caaaaaaatg aagaaaatta tactaacata aaattcagtg taactcaatg
      961 ttcaaatgcc tcactgattt gttatcaagg taatcaggtc ccactaaaat tttctctggc
     1021 aacgtataca aaaaaacctt tcctggtttt atgtcatgtt tcaatccgat gaatttgtta
     1081 tcgtttccaa gacaaatttt tttgcaattt ttcccactaa tgattccgta tgaagatttt
     1141 ttttcaccct gaatcttatt atttttaaac cagatatttt cggttacatc ttcaataaat
     1201 acttctgtat ttccttcttt ctgacaatta ttaacaatca tattggaatc aatcaagttt
     1261 ctatgtcctc ccattgtttt gtcggctttt ctgaaatata taccatgcct gaaatttttt
     1321 gttatcaaat tattcctgat aatatggtct gtatcccttg cgccaactga gatgccatcg
     1381 tttccatttt cagaaatttc acaattttca agtagggtgt aggtaacgcg taaacaataa
     1441 aaaacaccat cttttctatt acctgtaatt ttgcaattcc tcatcacaac tcctgcagaa
     1501 ccacttccag gatgtaagcc cgaaccataa tttttagaaa tatcacagtc ttcaatgatc
     1561 aaatttgtac attgctgaaa actgatacca tctccattat aatttttttc agtaacacct
     1621 tttattttta tcccgtgaca ttgccgaagg aacat
//
LOCUS       DAORYU010000297         1654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_49821_length_1654_cov_3.021263, whole genome shotgun sequence.
ACCESSION   DAORYU010000297 DAORYU010000000
VERSION     DAORYU010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49821_length_1654_cov_3.021263"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="PLW07_07460"
     CDS             <1..376
                     /locus_tag="PLW07_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50406.1"
                     /translation="FQIALALYFIAGSTQVARTVGSYRIMYAGIIKNSETIYYTSIYY
                     AWIGLAGGFAPLFAGYTLKILSDFHFQLSYITFDAYSILFMINFLCQAGAIIFLRKVK
                     SDKQFTTRELFFKLAQYIFEHQ"
     gene            417..1217
                     /locus_tag="PLW07_07465"
     CDS             417..1217
                     /locus_tag="PLW07_07465"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOL50407.1"
                     /translation="MRVKEVIDYIKEIAPFKDKFGEIPGDDSIVFGDENNIVKGIGVT
                     WSATSDVLKRAEQEKLNFIITHEFLFWGHSETIWFKSDRDILKKRPNAMRYEILQRNN
                     MTVYNTHKNWDAAEFWGMCDAFPKFLGFEKEIDRGRFIRVHEIPSMKAGELAEMIKEK
                     MKLPVIRIAGNPERKISRVATAVGGLGQIYGIAEEPARLGAEAVVFGEAVDYAIRCCV
                     ENDMVAIETSHVLSENPGVENLAKKLKEKFENTKVLFLDAGYPFNEVR"
     gene            1221..>1654
                     /locus_tag="PLW07_07470"
     CDS             1221..>1654
                     /locus_tag="PLW07_07470"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOL50408.1"
                     /translation="MKAGIIGLRMGKAHLVGYLQNNVEVAGICDIDQSLLTGVQKEFN
                     IPLATTDYHKLVAEKSIDLISVASPDYFHKEHCCSALLAGKHVLCEKPLALNLEDCIE
                     IIKAVKQSGKKFMIGQVCRFAPGFVLTKEIIDSGEIGELFFV"
BASE COUNT          556 a          263 c          357 g          478 t
ORIGIN      
        1 ttttcaaata gccctggctc tttactttat tgctggttca acacaggtag caaggactgt
       61 tggctcttac agaattatgt atgcagggat tataaaaaac agtgaaacaa tatattacac
      121 ttccatttac tatgcctgga ttgggcttgc aggaggtttt gcgccgcttt tcgcaggtta
      181 tacactaaaa atcctttctg atttccattt tcaactttca tatattactt ttgatgccta
      241 cagcatattg tttatgataa attttttgtg tcaggcagga gcaatcatct ttctcaggaa
      301 agtgaaatca gataaacagt ttacgacaag ggaattgttc tttaaactgg cacagtacat
      361 ttttgagcac caatagtata atagagttaa acgattacat ctgacaagga gacaaaatgc
      421 gggtgaaaga ggttattgat tatattaaag aaattgcacc tttcaaagac aaatttggag
      481 aaattcctgg agacgattca attgtatttg gtgatgaaaa caacattgta aaaggtatcg
      541 gggtaacatg gtctgcaaca agtgatgtat tgaaaagggc tgaacaggag aaacttaatt
      601 ttatcatcac ccatgaattt ttattctggg gacacagtga aacaatctgg ttcaaatcag
      661 acagggatat tttgaaaaaa agaccaaatg caatgagata tgaaattttg caaagaaata
      721 acatgactgt ctacaatacc cacaaaaatt gggatgcagc agaattctgg ggaatgtgcg
      781 atgcatttcc aaagttcctt ggttttgaaa aagaaatcga taggggaaga tttataagag
      841 ttcatgaaat cccatcaatg aaggcaggcg aacttgccga aatgataaaa gaaaaaatga
      901 agttacctgt aatcagaatc gcaggtaatc cagaaagaaa aatttccaga gtggcaacag
      961 cagttggtgg acttggccag atttatggca ttgcagaaga acccgcacgc cttggtgctg
     1021 aagcggttgt atttggtgaa gcagtggatt atgccatcag atgttgcgtt gaaaatgata
     1081 tggtcgcgat tgaaacaagt cacgtattga gtgaaaatcc tggtgttgaa aacctcgcaa
     1141 aaaaattgaa ggagaaattt gaaaatacaa aggtgttatt tctcgatgcc gggtatccct
     1201 tcaatgaggt gaggtgaaac atgaaagcag gaattattgg attgaggatg ggaaaagcgc
     1261 atcttgttgg ttatcttcag aacaatgttg aagttgccgg gatatgcgat attgaccaat
     1321 ctctattaac aggtgtacaa aaagaattca atataccact tgccacaact gattatcaca
     1381 aacttgtagc agaaaaaagc attgacctta tttctgttgc aagccctgat tattttcaca
     1441 aagaacattg ttgctctgca cttttagcag gaaagcatgt tttatgcgaa aaaccacttg
     1501 cattaaatct tgaagattgc attgaaatca tcaaggcagt caaacaatcc ggtaaaaaat
     1561 tcatgatcgg gcaggtatgt cgatttgcac cgggttttgt tcttacaaag gaaatcattg
     1621 attccggcga gataggtgaa ctattttttg ttga
//
LOCUS       DAORYU010000298         1649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_50038_length_1649_cov_3.515684, whole genome shotgun sequence.
ACCESSION   DAORYU010000298 DAORYU010000000
VERSION     DAORYU010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50038_length_1649_cov_3.515684"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..144)
                     /locus_tag="PLW07_07475"
     CDS             complement(<1..144)
                     /locus_tag="PLW07_07475"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOL50409.1"
                     /translation="MKKIGVLTSGGDAPGMNCAIRAVVRTAIGCGLEVSGIRRGFRGL
                     FEND"
     gene            complement(147..1220)
                     /locus_tag="PLW07_07480"
     CDS             complement(147..1220)
                     /locus_tag="PLW07_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50410.1"
                     /translation="MENFATYKDGVMISEEIFRTLAKYSSEGVFDVFASDQHSSYLKL
                     TDAYFKHIGVSKKTDDNDVKEVCLRFAKHLKGNSTAALFNHLAYLSPGFRETLGKDVM
                     LIGRLEDTNAESVDNGIGQIAKFAVEPEQVAHAVDGFKILVKLNPAHKKSWDKNIEFA
                     SKAFEASKKFNKPLFTETLLFQLPGESKMDFAKRLPDGLVAMAKDFGPIGHFYKTQVP
                     MLWMEEEGKVIQVASPQVVRDTAIEMEKISPRPMLLLSAAVDFEQYCAQYGIVSDLIS
                     GPMCGRAYFKEAFTDPSTKDWDSLEQAFVRIAIPRIRIIRRLAFAMSKPWWYKFTSMA
                     DSARSLIRTDTRKILSVRADFGY"
     gene            complement(1235..>1649)
                     /gene="purE"
                     /locus_tag="PLW07_07485"
     CDS             complement(1235..>1649)
                     /gene="purE"
                     /locus_tag="PLW07_07485"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013637678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOL50411.1"
                     /translation="LPFLDGAKEILDKFGVPYIVEVMSAHRSLDKTVEFSESARAKGI
                     QVIIACAGMAAHLPGVIAAKTTLPVIGVPLPTSEIKGIDALYSMVQMPSGVPVGTMAI
                     GKSGVKNAAIFAIEILALNDSTLYEKLKEYKDSLK"
BASE COUNT          465 a          410 c          282 g          492 t
ORIGIN      
        1 atcgttttca aaaaggcctc tgaaaccccg cctgatacca ctcacttcca gtccacaacc
       61 tattgctgtt cttacaaccg ctcttattgc acaattcatt ccaggagcat cgcccccact
      121 ggtcagaact ccaatttttt tcattttcaa tacccgaaat cagccctgac agaaagtatt
      181 tttcttgtgt ctgttcttat tagagacctt gcagaatctg ccattgaagt aaatttatac
      241 caccatggct ttgacatcgc aaaagcaagt cgcctgataa tcctgatgcg aggaattgca
      301 attctcacaa atgcttgttc aagactatcc cagtcctttg tcgaagggtc tgtaaatgct
      361 tccttgaaat acgcccttcc gcacatgggt cctgaaatca aatcactcac aatgccatac
      421 tgagcgcagt actgttcaaa atcaacagcg gcactcaaca ataacattgg acggggtgaa
      481 attttctcca tttcaattgc agtatcgcga acaacctgag gactcgccac ctgaatcacc
      541 ttaccttcct cttccatcca aagcatagga acctgggttt tgtaaaaatg ccctatgggt
      601 ccaaaatctt tcgccattgc gacaagacca tcaggcaatc tttttgcaaa atccatttta
      661 ctttcacctg ggagttgaaa tagcagtgtc tctgtaaaaa gcggtttgtt aaactttttc
      721 gacgcctcaa atgcctttga agcgaattca atgtttttat cccagctttt tttgtgcgct
      781 gggttaagtt tcaccagaat cttgaaacca tccacagcat gggcaacctg ttctggttca
      841 acagcaaatt tcgcaatctg gcctatccca ttatccacag attcagcatt ggtatcttca
      901 agccgtccaa tcagcataac atccttacca agtgtttctc tgaaaccagg agaaagatac
      961 gcaagatgat taaaaagcgc tgctgtgcta tttcccttca gatgctttgc aaacctcaaa
     1021 caaacttctt tcacatcatt gtcatccgtt tttttggaaa caccgatgtg tttgaaatac
     1081 gcgtctgtca gtttgaggta cgaactgtgc tggtctgacg caaaaacatc aaaaacccct
     1141 tcagaagaat attttgcaag ggttctgaaa atttcttcac ttatcatcac tccatcttta
     1201 tatgtggcaa aattttccat atgccacctc ctttttattt taaagaatcc ttgtattctt
     1261 ttagtttttc atacagagta ctgtcattga gtgcaagaat ttcaatagca aaaattgcag
     1321 catttttcac tccggacttg cctattgcca ttgtaccaac aggaacacct gatggcatct
     1381 gaaccattga gtaaagggca tcaattcctt tgatttcact tgttggaagc ggcaccccta
     1441 tcacaggaag cgtcgttttt gcggcaataa cccctggaag atgcgctgcc attccagcac
     1501 aggcaataat aacctgaatt ccctttgccc tggctgactc tgaaaactca actgttttat
     1561 caagacttct gtgagcagac atcacttcca caatatatgg aacaccaaat ttatcaagaa
     1621 tttcctttgc gccgtcaagg aaaggtaaa
//
LOCUS       DAORYU010000299         1617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_51462_length_1617_cov_3.918054, whole genome shotgun sequence.
ACCESSION   DAORYU010000299 DAORYU010000000
VERSION     DAORYU010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51462_length_1617_cov_3.918054"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..>1617)
                     /locus_tag="PLW07_07490"
     CDS             complement(41..>1617)
                     /locus_tag="PLW07_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50412.1"
                     /translation="VAKQIRESKPGFHGNTLQAALDIACTLLEKKSGYKRIFIITDFQ
                     KSGWEKVTLTGKINADITIVDAGEDYRENLTIKDFYPVPGKNIYICEIINWGRQQVTS
                     ELNITGENFQTIKQVNLPGEKTTEVEIKITNDCQSLKAQLPYPDVLKIDNQFFLQRET
                     SGEKKVLLTGSDEASLFYIESSVTSSGTIAADIKKINEIPDTPLEQYRTIILVNPEKM
                     EPYIRGRIYQYVSDGGTLIYFPGERIAPEDFNNDWFHKEKNIFIMPAHLDRKNNFLKP
                     AGVAWIATGHPLFTEFGEKAFDYLKTTRFNACYSVREITGDILMRLDNGNIALIEKRI
                     GKGKVFLFTFTPYQPWTNFHTKPFFPVMMTVMIEYLSGITSSTSIGNTITVKGSEYTD
                     SVNIINPEGTINTIKNTGKTPVSYVPDLPGIWTARFVAKGKEQTQMVSVNVPYTEGNL
                     SKISYGEIRTILRKTRVNFVSRNNMEKFIAMETSGGNLMMVFLELAMALLIMELMLSN
                     LFVFFKEKGTHASHKL"
BASE COUNT          455 a          300 c          290 g          572 t
ORIGIN      
        1 tgcaaaaaac agtatcacca caatggtagg cgccggacaa ttaaagttta tgtgaagcat
       61 gtgttccctt ttctttgaaa aaaacaaaaa ggttacttaa cattagttcc ataattaaaa
      121 gagccattgc aagttcaagg aaaaccatca tcaggttacc gcctgaagtt tccatggcaa
      181 taaatttttc catattattt ctgctaacga aatttactct ggttttcctt agaattgttc
      241 ttatttctcc atacgaaatt tttgaaagat ttccttctgt gtaagggaca ttgactgaca
      301 ccatttgtgt ttgttctttt ccttttgcaa caaaacgcgc tgtccagata cctggcaggt
      361 caggcacata tgaaacaggt gttttccctg tgttttttat tgtgtttatc gtgccttcag
      421 gattaatgat atttacagaa tcagtatatt ctgacccttt aacagtaatg gtgttaccga
      481 tagatgttga agaagtgatt ccagagagat attcaatcat tactgtcatc ataacaggaa
      541 aaaatggttt ggtatggaag tttgtccatg gttgatacgg cgtaaatgtg aacagaaaca
      601 ctttcccttt tccaattctt ttttcaatta aggcaatatt accattgtca agtctcatca
      661 ggatgtctcc tgtgatttcc ctgacagaat agcaggcatt aaaccttgtt gttttcagat
      721 aatcgaatgc tttttcgcca aattctgtga aaaggggatg tccggttgca atccaggcaa
      781 caccagcagg ttttaagaaa ttattttttc tatcaaggtg tgctggcatg ataaaaatgt
      841 ttttctcttt gtgaaaccag tcattattga aatcttctgg cgcaattctt tctccaggga
      901 aatagataag ggtaccgcca tcagaaacgt actgataaat tcttcccctg atataaggtt
      961 ccattttttc tggattcacc agaatgattg tcctgtactg ctcaagaggt gtatcaggaa
     1021 tttcattgat tttcttgatg tcagcagcaa ttgtcccaga agaagtaact gaagattcaa
     1081 tataaaatag ggatgcttca tcagaccctg ttaacagaac tttcttttca ccgcttgttt
     1141 ctctctgaag aaaaaactgg ttatcaattt tcaaaacatc tggatagggt aattgtgctt
     1201 tcaatgattg acaatcattt gtaattttga tttccacttc tgtggttttt tctcctggaa
     1261 gatttacctg ttttattgtt tgaaaatttt cgccagtaat gtttaattca gatgtaacct
     1321 gttgcctgcc ccagtttata atttcacaga tatagatatt tttacccggt acaggataaa
     1381 aatccttgat ggtcaagttt tctcgataat cttcacctgc atcaactatc gtaatatctg
     1441 cattgatttt tccagtcagg gttacttttt cccagccgga tttctggaaa tctgttatga
     1501 taaatattct tttataaccg gatttttttt caagcagggt acatgcgata tctaatgctg
     1561 cctgcaaagt attgccgtga aaacccggtt tgctttcccg tatttgtttt gccacat
//
LOCUS       DAORYU010000300         1589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_52822_length_1589_cov_4.215124, whole genome shotgun sequence.
ACCESSION   DAORYU010000300 DAORYU010000000
VERSION     DAORYU010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52822_length_1589_cov_4.215124"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="PLW07_07495"
     CDS             complement(<1..750)
                     /locus_tag="PLW07_07495"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HOL50413.1"
                     /translation="MRKLFHTVLTLSFALAIQAHSQAQWTVMIYLDGDNNLEKYAIND
                     FLEMATVGSTAQINFVVQLDRISGYETRYGNWTNTQRFRITRDMEPTIDNAISDWGDG
                     YGGRETNSGNPEVLASFIQWAKQNYPAAHYALILWDHGDGWKSLSDVAESIEQQLKQT
                     GLAREERVALEKTLKELKRKIHSMRYQKSVCYDYTSSDELTLKELKQALFSENCNVNM
                     IAFDACLMGMTEIAYEIRNFANYMVASEETIM"
     gene            complement(762..>1589)
                     /locus_tag="PLW07_07500"
     CDS             complement(762..>1589)
                     /locus_tag="PLW07_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50414.1"
                     /translation="CNVDAEILISDTEKVVCTGCEGTGSAYNTKNSSISFVIDKDSKI
                     TWKWKKVPVEPGLVVETPDEVNFEFSKQVLSIPIKIRFLGGLTDTVFLHTAGNFDITL
                     SDTCLNPEKKQTSAIIKKSDNLEAGKYSISIYGRTKNKKLQAEKNITVIVPATVFFGE
                     ATTNETEKLVTIPVMVNGNVKNISSFEIIISIPENSKFIRIESRLGTKIMSGIVQKGK
                     YLKISGGLIPPSDIKNEKLFNVILSPSKMIHGDKFNPVRISLWNENGEIIPVQTLIK"
BASE COUNT          439 a          309 c          267 g          574 t
ORIGIN      
        1 catgattgtt tcttcagagg caaccatata atttgcaaag tttcttattt catatgcgat
       61 ttcagtcatt cccatcagac aggcatcaaa agcaatcata ttaacattgc agttctcaga
      121 aaaaagcgcc tgttttagtt ctttcaaagt aagttcatct gaggatgtgt aatcataaca
      181 cacacttttt tgataacgca tggaatgaat cttccttttt aactccttta aggttttttc
      241 aagagcgacc ctttcttcgc gagcaagtcc agtttgtttt aactgctgtt caatactttc
      301 tgcaacatcg cttagagatt tccaaccatc accgtggtcc cataaaatta aggcataatg
      361 cgctgctgga taattttgtt ttgcccattg tataaaagaa gcaagtactt caggatttcc
      421 agaattcgtt tctcttccgc catagccgtc gccccaatca gaaattgcat tatcaatggt
      481 tggttccata tctctggtta ttctgaatct ctgggtattt gtccagttgc cgtacctggt
      541 ctcgtaacct gaaattctat caagttggac aacaaagttt atctgtgctg tcgaaccaac
      601 tgttgccatt tcaagaaaat cattgatagc atacttttca agattattgt ctccgtcaag
      661 atatatcatc actgtccatt gtgcctggct atgtgcctga atagcaaggg cgaatgaaag
      721 tgtaagtaca gtatggaaaa gttttctcaa aaatctgtgt atcatttgat tagggtttga
      781 actggtataa tttctccatt ttcattccac aacgaaatgc gaacaggatt gaatttatct
      841 ccatgaatca tctttgaagg cgaaagaata acattgaaaa gtttttcgtt tttgatatct
      901 gatggcggta ttaatccgcc actgattttc agatactttc ctttttgaac aataccactc
      961 atgatttttg tccctaatct ggattcgatt ctgataaatt tgctgttttc cgggatacta
     1021 attattattt caaaacttga aatgtttttc acattaccat taaccattac aggaatggta
     1081 accagttttt ctgtttcgtt tgtggttgct tctccaaaaa aaacggttgc aggtactatc
     1141 acagtaatat ttttttctgc ctgtaatttc ttattttttg ttctgccata gattgatatt
     1201 gagtatttac ctgcttcaag attgtcagac tttttgataa tggctgatgt ttgttttttt
     1261 tctggattca aacatgtatc agagagcgtg atatcaaaat tgcctgctgt gtgcaaaaat
     1321 actgtgtcgg taaggcctcc cagaaatctt attttaatcg ggatagaaag gacctgtttt
     1381 gaaaactcaa aattgacttc atctggagtt tctactacca aacctggttc tactggaact
     1441 tttttccatt tccatgtaat ttttgagtcc ttatcaataa caaaagaaat actgctattt
     1501 ttcgtattat acgcagaacc tgttccttcg catcctgtgc acactacttt ttctgtatca
     1561 ctgatgagaa tttctgcatc cacattaca
//
LOCUS       DAORYU010000301         1585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_53040_length_1585_cov_3.501961, whole genome shotgun sequence.
ACCESSION   DAORYU010000301 DAORYU010000000
VERSION     DAORYU010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53040_length_1585_cov_3.501961"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..>1585
                     /locus_tag="PLW07_07505"
     CDS             33..>1585
                     /locus_tag="PLW07_07505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOL50415.1"
                     /translation="MKSQKIVNEVPGSRTILDVVPEGTQVTEEDVKNGKVLIKLDSRD
                     IENDLDQQKVSLETSYASFKEAEENLEIQKKQNESDINQGQLKVKFAKMDMEKYLGAE
                     IAEKLLTSKTTSIDYQELLKNPALGGDALNKKRQYENKIDLAKEELARSQDTVDWSTK
                     LAEKGYVTKSELEADKLALQQKNVSLEQAKLDYELFKAYDFPRQIEQLLSNYREAVAE
                     LERTKSKCQSRIIQAEANVKSRKATYMLTKARLDTTIQNLEKCTIRATQPGFVVYATT
                     GRFFVESPISAGTSVRMYQELLNLPDFSTMGVEIKIHESSVEKVKAGLPATIKVDAFP
                     NQTFTGKVQSVALMPDPTLKWLNPDLNVYVAQISFDKSYDYLRPGMSAQVEVIVNTLK
                     NVLVIPLVAVNFSEGKAYVNVLRGRSIETRQIEIGESNEREVEVKKGLKEGDIIVVSA
                     IGKPAGTKKQVETEKETEQKTQQPASDAGSQSNQPVVQQPQAEQKPQETSTPSRRIER
                     TTSAQGTEPS"
BASE COUNT          592 a          294 c          350 g          349 t
ORIGIN      
        1 tttcagttat tgagggaggt aatctgaaag cattgaaatc acaaaaaatt gtcaatgaag
       61 tgcctggttc acgaacaata cttgatgtgg ttccagaagg aacacaggtt actgaggagg
      121 acgtgaaaaa tggaaaggtt ttgattaaac ttgactcaag agacattgaa aacgaccttg
      181 accagcagaa ggtcagtctg gaaacaagtt atgcgtcatt caaggaggca gaagaaaacc
      241 ttgaaattca aaaaaaacaa aatgaaagcg acatcaatca gggacagttg aaggtaaaat
      301 ttgccaaaat ggacatggaa aagtacttag gagcagaaat cgcagagaaa cttcttacat
      361 caaaaacaac cagtattgac tatcaggaac ttttgaaaaa tccagccctt ggcggagacg
      421 cgttgaacaa aaaaagacaa tacgaaaaca aaattgacct tgcaaaagaa gaacttgcca
      481 ggtcacagga tacagtggac tggagtacaa aacttgctga aaagggttat gtaacaaaaa
      541 gcgaacttga ggcggataaa ctggcgcttc agcagaaaaa tgtttccctt gaacaggcaa
      601 aactggatta tgaattattc aaggcatatg atttcccgcg ccagattgag cagcttttat
      661 caaactatcg tgaggcagtc gctgaactgg aaagaacaaa atcaaaatgt cagtcaagga
      721 ttattcaggc agaagcaaat gtgaaaagta gaaaggcaac atacatgctt acaaaagcac
      781 ggcttgacac aacgattcaa aatcttgaaa aatgtacaat cagggcaaca caaccaggtt
      841 ttgttgtata tgcaacaaca ggaagatttt ttgttgagtc gcccatctca gcaggaacct
      901 ctgtaagaat gtatcaggaa ttactcaacc ttcctgactt ttcaacaatg ggcgttgaga
      961 taaaaattca tgagtcaagc gttgaaaagg taaaggcagg tcttccagca accattaagg
     1021 ttgatgcttt tccaaatcaa acctttactg gaaaggttca gagtgttgcc ttgatgccag
     1081 acccgacttt gaaatggctg aaccctgatt tgaatgtata tgttgcccag atatcatttg
     1141 acaaaagtta tgattacctc aggccaggaa tgagcgccca ggttgaagtt attgtcaata
     1201 ctctaaaaaa cgtactggtg attccccttg tcgctgtcaa tttcagtgaa ggaaaggcat
     1261 atgtaaatgt tctgcgtggc agaagtattg aaacaagaca gattgaaatc ggagaaagca
     1321 atgaaagaga agttgaagtg aaaaagggat tgaaggaagg agacatcatt gttgtttctg
     1381 ccataggcaa acccgcagga acaaagaaac aggttgaaac agaaaaagaa acagaacaaa
     1441 aaacacagca gcccgcatca gatgctggtt cccagagtaa tcaacccgtt gttcaacaac
     1501 cacaggcaga acaaaaacca caggaaacct caacaccttc acgaagaata gaaagaacca
     1561 catctgccca ggggacagaa ccttc
//
LOCUS       DAORYU010000302         1572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_53646_length_1572_cov_5.495056, whole genome shotgun sequence.
ACCESSION   DAORYU010000302 DAORYU010000000
VERSION     DAORYU010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53646_length_1572_cov_5.495056"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1572)
                     /gene="mgtA"
                     /locus_tag="PLW07_07510"
     CDS             complement(<1..>1572)
                     /gene="mgtA"
                     /locus_tag="PLW07_07510"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020473672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HOL50416.1"
                     /translation="VSCKDLFVNQASLTGESFPVEKIAIPVVVANSQPSELKNIAFMG
                     SSIVSGTGLGVVIKTGLATQFGMVSYKLATMTEETNFERGVRSFTWLMIRFMLVLVVI
                     IFFINAMTKGDFLQSFLFALAVSVGLTPEMLPMLVAINLSKGAIAMSRKDVIVKRLSS
                     IQNFGAMDVLCTDKTGTLTLDEIILEIYCDVTREESEEVLYLAYINSCYQTGLKNILD
                     RAVLKYKELQVEQYKKIDEIPFDFSRKMMSVVVEIEGKHRLITKGAPEEVFKKCVKYE
                     LDGELFDIDPLVLTDLKLEYEHLSAQGFRVLAIAYRDYEIPKEAYTKNDEVEMVLKGY
                     VAFLDPPKPTAKETIEALRKRGIELKVLTGDNELVTRHICNEVGLDVRNMLTGEHVET
                     MGDNELVDAVKSVTVFSRLSPLQKERIIRALHKNNHTVGYLGDGINDALALKAADVGI
                     SVDNAVDIAKESADIILLQKSLTVLEDGVIEGRKTCGNILKYIKMGSSSNFGNMFSMT
                     GASIFLPFLPMLPIQ"
BASE COUNT          470 a          364 c          248 g          490 t
ORIGIN      
        1 tctgaattgg aagcattggc aggaatggca gaaatatgct tgcgcctgtc atactaaaca
       61 tgttaccaaa atttgaactt gaacccattt tgatatattt cagaatgttt ccgcatgttt
      121 ttcttccttc aataacacca tcttcaagaa ccgtcaaact tttttgaagg agaataatat
      181 ccgctgactc ttttgcaata tccacagcat tatcaactga aattccaaca tcagcggcct
      241 ttaaagcaag agcatcgttt ataccatctc caagatatcc tactgtatga ttgtttttat
      301 gcaatgccct gattattctt tccttttgca atggtgacaa tcttgaaaaa acggttacac
      361 tttttacagc atctaccagt tcattatcac ccattgtttc cacgtgttct cctgtcagca
      421 tgtttcttac gtcaagacca acctcattac atatgtgcct tgtaacaagt tcattgtcgc
      481 ctgttaaaac ctttaattca attccccttt ttctcaatgc ttctattgtt tccttcgcgg
      541 ttggtttggg tggatcaaga aatgcgacat agcccttgag aaccatttcg acctcatcgt
      601 tttttgtata cgcctctttc ggtatttcat aatccctgta agcaattgca agaaccctga
      661 aaccctgagc acttaaatgc tcatattcaa gtttcaaatc agtgagaaca agagggtcaa
      721 tatcaaaaag ctctccatcg agttcatatt tcacacattt cttgaaaact tcttccggcg
      781 cccctttggt aatcaaacga tgtttgcctt caatttcaac gacgacagac atcattttcc
      841 gcgaaaaatc aaacggtatc tcgtctatct ttttatactg ttcaacctgc aactctttat
      901 atttcaaaac agccctgtca agaatgtttt ttaaacctgt ttgatagcaa ctgttaatat
      961 aagcaagata aaggacttcc tcgctttcct ctctcgtcac atcacaatag atttcaagaa
     1021 tgatttcatc aagggtcaat gtgccggttt tgtcggtaca cagtacgtcc attgctccaa
     1081 aattttgaat tgaactcaat cgctttacaa ttacgtcctt tctcgacatc gcaatcgcac
     1141 cctttgaaag gttaattgcc acaagcatcg gaagcatttc aggagtaagc ccaacagaaa
     1201 cagccagagc aaaaaggaaa gattgtagaa aatcaccctt tgtcattgcg ttgataaaaa
     1261 atattattac gaccagaaca agcataaaac gtatcatcag ccaggtaaaa gaacggactc
     1321 ccctttcaaa atttgtctct tccgtcattg ttgccagttt gtaggaaacc attccaaatt
     1381 gagtggccag ccctgtttta atcacaaccc caagaccagt gccactgaca atacttgaac
     1441 ccataaatgc aatatttttc agttctgatg gttgactatt cgcaacaaca acagggattg
     1501 caattttttc aacagggaag gattctcctg taagtgacgc ctggttaaca aataaatcct
     1561 tacacgaaac aa
//
LOCUS       DAORYU010000303         1554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_54521_length_1554_cov_2.603736, whole genome shotgun sequence.
ACCESSION   DAORYU010000303 DAORYU010000000
VERSION     DAORYU010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54521_length_1554_cov_2.603736"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            189..>1554
                     /locus_tag="PLW07_07515"
     CDS             189..>1554
                     /locus_tag="PLW07_07515"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOL50417.1"
                     /translation="MKKIIFSLLLMMIATGISCGEELKVFGQDFFSNVNLSGVEFSRF
                     IPDKYYLGPGDQIEISVWGVTEFRQQMEIDREGSIIVPRIGKIYLSGKNLIQAKKYLQ
                     DIFSRTYRDIKLELTLVKPRQITVFVMGEVVKPGTYAITTSSSILEILGLAGGITERG
                     SMRKVSIVHRDGTKQEIDLYPVFFGDGLPDITIQAEDVVYVPLATQFVSISGAVRRPA
                     KYEMTEPLSLKKLIEYAGGFLPEADTKRIAVFRNDTIAGRTIIDIFPQNQKQDALEKF
                     FLTDGDWIEVSFISKDVVDCVFVEGIIKNPGKYGWKQGLTVSDIVKESDFLPETSQEQ
                     AEIIRENTSGAREIIQFSLVDAVRKIKDVELQPRDRIVIRSKQQPVKTVTLSGEVKFP
                     GQYVISSGETLSSVINRAGGFTSQAYLPATVFTRVSVRQREKQELEKFIMEKQATLEK
                     EAGRT"
BASE COUNT          500 a          271 c          374 g          409 t
ORIGIN      
        1 ctgtgtatga cgaaagccat atgaaacttt atgttgatgc gtttgaaaag gttgcacagg
       61 catatatgaa atagtacgcc acagggcaca ttctttctat cactatcaga gaggtgtgcc
      121 cgtttttctt ttggtgaaca cttgacgaat ttccaattga tggtaattta tgcttgtgag
      181 gacataaaat gaaaaaaatt atcttttctc ttttgttgat gatgattgcc acagggatat
      241 cctgcggaga agaattgaaa gtgtttggcc aggacttttt ttcaaatgtc aatctcagtg
      301 gtgttgagtt cagtagattt attcctgaca aatattacct gggacctgga gatcagatag
      361 aaatatctgt ctggggagta actgaatttc gtcagcagat ggaaattgac agagaaggaa
      421 gcatcattgt tccgagaatt ggcaaaatct acctatcagg gaaaaatctt attcaggcaa
      481 aaaaatacct tcaggacatt ttttccagaa cttacaggga cataaaactt gaactgacgc
      541 ttgtaaaacc ccgtcagatc actgtttttg tgatgggcga ggttgtaaaa ccaggcacat
      601 acgcaatcac gacttcaagt tccatacttg agattcttgg tcttgctgga ggaattactg
      661 aaagaggaag catgaggaaa gtcagcattg ttcacaggga cggcacaaaa caggaaatag
      721 acctgtatcc tgtattcttt ggagacggac tgcctgatat cacaatccag gcagaagatg
      781 ttgtgtatgt accgctcgcg acacaatttg taagtatctc aggcgcagtg agaaggcctg
      841 caaagtatga gatgacagag ccgctttctc ttaaaaaact gattgaatat gcagggggct
      901 ttctgccaga agcagacacg aaaagaattg cagtattcag aaacgataca attgctggaa
      961 gaacaattat tgatattttc ccgcagaacc agaaacaaga tgcccttgaa aaatttttcc
     1021 tcacagacgg agactggatt gaggtttcat ttatcagcaa ggatgtcgtt gactgcgtgt
     1081 ttgtggaagg gataatcaaa aatccaggta aatatggctg gaaacagggc ttaaccgtat
     1141 ctgatattgt gaaagaatct gattttcttc ctgaaacaag ccaggaacag gcagaaatca
     1201 tcagggaaaa cacatctgga gcaagagaaa ttatccagtt ttcgcttgta gacgctgtaa
     1261 gaaagattaa ggatgtggaa ttacaaccaa gggacaggat tgtgataagg tcaaagcagc
     1321 agccagtgaa aacagttacc ctttcaggtg aggtgaaatt tcccggtcag tatgtgattt
     1381 cgtcaggaga aaccttaagt agtgtcatta atcgtgcagg tggatttacc agccaggcgt
     1441 atttgccggc tactgtgttc acaagggtat ctgtaaggca gagagaaaaa caggaactgg
     1501 aaaagttcat catggaaaaa caggcaacgc ttgagaaaga agcaggaagg acag
//
LOCUS       DAORYU010000304         1549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_54762_length_1549_cov_6.726908, whole genome shotgun sequence.
ACCESSION   DAORYU010000304 DAORYU010000000
VERSION     DAORYU010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54762_length_1549_cov_6.726908"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1549
                     /locus_tag="PLW07_07520"
     CDS             <1..>1549
                     /locus_tag="PLW07_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50418.1"
                     /translation="CQVVDEPLGPRYSLGMTLWHMGHNPEMIEKFHQYLKQLNVSPEK
                     LLPSSNRPQSVVKILIEPELMGNENLSGWKINGNYITSGATDAGPTVSIPLNIPKAGL
                     YRFWISYYGWMNHRGVTFIKFYKKGQEHLGPIFQMDEFYDQMPEKEGLQWKDMLVDLP
                     EGELIVKLGHITRWWHGKGGYASRWVDCLYLTDEIWKNAPETEQLKKIKESSKPQGIQ
                     WTIIESFDKQEYENWKWWQVRPISWEDAEKNPKLFDLSRKFWNQVIEELSKKQYSEDS
                     LPDYREPERQVVFNEVWNMVANPVRAKRQIDTLTSDISKKPLGYHYVWHDVGSNIEGL
                     REDGRYEKGSKYEKYGGWYGGPGRLIAGYGSPRGTVSTEVDIKAPGTYQCWILSDRTN
                     LSYTAPYFCKAYVDGQEQFTYHHKDKIPNIWMKMGEIKVSKPTKVRFDFILDGAGAGS
                     TYRRIYKLFLVDNPEIIPEGTVQPPWTIEMYREKAKQLGAKPDDKLLVWLQENPFRRL
                     SQEVWADK"
BASE COUNT          520 a          262 c          371 g          396 t
ORIGIN      
        1 tgtcaggttg tggacgagcc acttgggccg cggtattctc tcggaatgac attgtggcat
       61 atgggacaca atcctgaaat gattgaaaaa tttcatcagt atttgaaaca attaaacgtg
      121 agccctgaaa aacttttgcc atcttccaat cgtccgcaat cagttgtcaa aatacttatt
      181 gaaccagaac tgatgggaaa tgaaaatttg agtggatgga aaatcaatgg caattatatc
      241 acaagcggtg ctactgatgc tgggccaact gtctctattc ctttgaatat tccaaaagca
      301 ggcctatata gattctggat ttcctattac ggctggatga atcatcgtgg tgtgaccttc
      361 attaagtttt acaaaaaagg acaggaacat cttggcccga ttttccagat ggacgaattt
      421 tatgaccaga tgcctgaaaa agaagggttg caatggaagg atatgcttgt ggatcttcca
      481 gagggtgagt taattgtgaa acttgggcat atcacaagat ggtggcatgg aaaaggtggc
      541 tatgcttctc gatgggttga ttgcctttat ttaactgacg aaatctggaa aaacgcacca
      601 gagactgaac aattgaaaaa aatcaaggaa agttcaaaac cccagggaat tcaatggaca
      661 attattgagt catttgacaa gcaggaatat gaaaactgga agtggtggca ggtcagaccc
      721 atttcatggg aagatgcaga aaaaaatcca aaactttttg atttgagtag aaaattctgg
      781 aatcaggtga ttgaagaatt gagcaagaaa caatacagtg aagacagtct tcctgattac
      841 agagagccag aaagacaggt cgttttcaat gaggtgtgga acatggtcgc aaatccagta
      901 agagcaaaaa gacagattga tactttaacc agcgacatat caaaaaagcc acttggatat
      961 cattatgtgt ggcatgatgt gggtagcaat atcgagggct tacgggaaga tggtagatat
     1021 gaaaaaggaa gtaaatacga aaagtatggt gggtggtatg gaggacccgg aagattgatt
     1081 gcgggatatg gaagtccgag gggaactgtt tccacagaag ttgacatcaa agcccctgga
     1141 acataccagt gctggatact ttctgacagg acaaatcttt catatactgc gccttatttc
     1201 tgcaaggcgt atgttgacgg acaggagcaa tttacatatc atcacaaaga caaaatccca
     1261 aatatctgga tgaaaatggg tgaaatcaaa gtttcaaaac caacaaaggt aagatttgac
     1321 tttattcttg atggcgcagg agcaggcagt acatatagaa ggatttataa acttttcctt
     1381 gttgacaatc cggaaattat tcctgaaggt actgtgcagc caccatggac tattgaaatg
     1441 tacagagaga aagcaaaaca acttggtgca aaaccagatg acaaacttct tgtatggctt
     1501 caggaaaatc ctttcaggcg gctttctcag gaggtctggg cagataaaa
//
LOCUS       DAORYU010000305         1539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_55296_length_1539_cov_3.986523, whole genome shotgun sequence.
ACCESSION   DAORYU010000305 DAORYU010000000
VERSION     DAORYU010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55296_length_1539_cov_3.986523"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>963)
                     /locus_tag="PLW07_07525"
     CDS             complement(<1..>963)
                     /locus_tag="PLW07_07525"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOL50419.1"
                     /translation="TDSKKIKDYDAVIVFLKKILPDELKKLDDYAKAGGNLLLICPPA
                     GSVSALKEITGCNVLQDKGLQQFTVFAEEKNHYITQRFPAEFQINDLLFDVEPLHSTI
                     IFSTYIGNKKIPVVSVRECGKGKFSCVFAGNTTQTWYNFDFRKIVIRCIAWMLGEKQK
                     EKTIHCGLIGYGPMFGMGKGHASWINATTGMKTVAVCDTNPERVEAARQELPDLLGYF
                     TSTEEMLKMKEIDLVVGIVPHNVHCEVALKGFAYGKHVILEKPFCISVEEADKMIETA
                     RKNKLMLSVFHNRRWDGDYIAIKNIVNNGLIGDIFHIECFIGNY"
     assembly_gap    964..973
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1095..>1539)
                     /locus_tag="PLW07_07530"
     CDS             complement(1095..>1539)
                     /locus_tag="PLW07_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50420.1"
                     /translation="LSFAATPLFWWHNFIEDKNLYFHYKALAEYMAGQDRLKEKLDPK
                     QVKITGENETNIKSLCLANQEKALIWVYGKDRLNKPPSDSDPVLTKNNQCLIEGLIPG
                     DYVIEFWDTYAGKIVQTKIEKNEGSMSFLLPETNRDFAIKIYRKS"
BASE COUNT          418 a          337 c          245 g          529 t
ORIGIN      
        1 catagttacc aataaaacac tctatatgga aaatgtctcc aatcaaacca ttgttcacta
       61 tattctttat ggcgatgtaa tccccatccc atcttctatt atggaatacg ctgagcatta
      121 acttgttttt tcttgccgtt tcaatcattt tgtctgcctc ttccacgctg atacagaacg
      181 gcttttcaag aattacatgt ttcccgtaag caaaaccttt caatgcgacc tcgcagtgca
      241 cattgtgcgg aacaatgccc acaaccaggt ctatttcttt cattttcagc atttcttcag
      301 tactggtgaa atatcccagc aaatcaggca attcctgtct ggctgcctct accctttcag
      361 ggtttgtgtc gcatacagcc acagttttca ttccagttgt tgcattaatc cagcttgcgt
      421 gtccctttcc catgccaaac atgggtccat aaccgataag tccgcaatga attgtttttt
      481 ctttttgttt ttctccaagc atccaggcaa tgcatctgat aacaattttc ctgaaatcaa
      541 agttgtacca tgtctgcgtg gtgttaccag caaatacaca ggaaaatttt cctttaccac
      601 attctcggac agaaacaaca ggaatcttct tgtttccgat atacgttgaa aaaatgattg
      661 tcgagtgtaa tggttcaaca tcaaaaagta aatcattaat ttgaaattca gcaggaaacc
      721 tctgggtaat ataatgattt ttttcttctg caaaaacagt aaattgttga agtcctttat
      781 cctgcaagac attgcaacct gtgatttctt ttaatgcgct tacacttccg gcagggggac
      841 atatcaataa taaattcccg cctgcttttg cataatcgtc aagttttttc aattcatctg
      901 gtaagatttt tttcagaaaa acaatgaccg catcataatc ctttattttt ttgctgtctg
      961 tttnnnnnnn nnnaaagaaa atcttccatc accttcaaac aatttttcaa tttctgattt
     1021 tgcccatacg gaaatatggg attcatcccg gtcatgaaga tacagaactt ttttcatcat
     1081 agcccccttt tcctttatga ttttctgtaa attttgattg caaaatccct gtttgtttca
     1141 gggagtaaaa aactcatact gccttcattt ttttcaattt ttgtctgtac aattttgcca
     1201 gcgtatgtat cccagaattc tatcacataa tcgccaggaa tcaagccctc aatcaaacat
     1261 tgattgttct ttgtcaaaac cgggtcagaa tcagatggtg gtttgttcaa cctatccttg
     1321 ccatacaccc atatcagtgc tttttcctgg tttgcaagac acagtgattt aatattggtt
     1381 tcattttcgc ctgtgatttt cacctgtttc ggatcaagtt tttccttaag tctgtcctga
     1441 cctgccatgt attctgcaag ggctttgtaa tgaaagtaga gatttttatc ctcaataaaa
     1501 ttgtgccacc agaacagtgg agtggctgca aacgaaaga
//
LOCUS       DAORYU010000306         1539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_55320_length_1539_cov_2.276280, whole genome shotgun sequence.
ACCESSION   DAORYU010000306 DAORYU010000000
VERSION     DAORYU010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55320_length_1539_cov_2.276280"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="PLW07_07535"
     CDS             complement(<1..463)
                     /locus_tag="PLW07_07535"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOL50421.1"
                     /translation="MSKTTEKGKVVMEKKNAKNKEISDRPNVILILTDDLAWSDLGCY
                     GSKFNLTPNLDNLASKGIRFINAYASSPVCSPSRASLLTGKSPARLHFTHITQTGISR
                     NLYWESPKFINYLPTAESTIAIELKKKGYATAIIGKWHIGGYGKAPEETGDP"
     gene            complement(567..>1539)
                     /locus_tag="PLW07_07540"
     CDS             complement(567..>1539)
                     /locus_tag="PLW07_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50422.1"
                     /translation="HIAHTGSGIHAFDASWFYPALRIANVPVDVVTDQQVKSGILNEY
                     KALILYNFNYASRSLWNAIDKFSKTPGKIVFADNQSNLLPRNTIKIPFNALGDAPPQG
                     IEKYKNIPYYGTRNMAWMAGQLREIVSSKIPPGEIRVSGSELVAPFLLHSRGKGQARM
                     LILVNYDTTSSQAVQVFLQTESDTVIYEFDTGKEIGLATPTQPVLWNTTIKPADWKIY
                     LLCKGKIEKIDIKAGYSDDEFQFQIKILDENNRPLRAGLPVKLELVDPEGSPISEYVK
                     YSAINPDDGVLKIDIPRAKLMDFSGTWTAKVEELITGKKSSVKFSVK"
BASE COUNT          446 a          322 c          256 g          515 t
ORIGIN      
        1 caggatctcc tgtttcttct ggtgctttcc catagccacc tatatgccat tttcctatta
       61 ttgcagtagc atatcctttc ttttttaatt caattgctat tgttgactct gcagttggaa
      121 gataatttat aaattttggt gattcccagt aaagattcct tgaaattcct gtctgagtaa
      181 tatgagtgaa atgaagtcga gctggacttt ttcctgttaa aagagaggca cgggaaggag
      241 aacataccgg tgaagaagca taagcattta taaaacgaat tcccttcgat gcaaggttat
      301 caagatttgg agttaaattg aatttagaac cgtaacagcc aaggtcactc caagcaaggt
      361 catcagttaa tatcagaatt acattcggtc tgtccgagat ttctttgttt ttagcattct
      421 ttttttccat aacgaccttc cccttttcag ttgtttttga caccgtttct ccagtacatc
      481 cagtaaaaca tacagtaatc atcgcaaata atgcaaccat taaaaaataa aatccatgaa
      541 agttcaaaat tgaaaccatg ttgtcctcac tttacactaa atttcacact ggattttttc
      601 cctgtaatca gctcttctac cttcgctgtc catgttccag aaaaatccat aagtttcgct
      661 cttggtatat ctattttcaa caccccatca tctggattaa ttgcagaata tttcacatac
      721 tcagaaatag gactcccttc aggatctacc aactcaagtt taactggtaa tcctgccctt
      781 aaaggtcggt tattttcgtc cagaattttt atctggaatt gaaattcatc atcagaataa
      841 ccggctttta tatcaatttt ttcaatttta cctttacata ataagtaaat tttccagtcc
      901 gctggtttaa tagtcgtatt ccaaagaaca ggttgagtcg gagttgcaag tccgatttcc
      961 ttcccagtgt caaactcata tatgacggta tcagattctg tctgcaaaaa tacctggacc
     1021 gcctgtgaag acgttgtatc atagtttaca agaatcaaca ttcttgcctg cccttttcct
     1081 ctactatgaa gtaaaaaagg agcaacaagt tcagacccac tcacccttat ttcccctgga
     1141 ggaatttttg atgaaactat ttctctcagt tgacctgcca tccacgccat gtttcttgtt
     1201 ccatagtatg gaatattttt gtacttctca attccctgag gcggagcatc gccaagcgca
     1261 ttgaatggta ttttgattgt atttcgagga agaaggtttg attgattatc agcgaataca
     1321 attttcccgg gcgtttttga aaatttatca atagcattcc acaaacttct acttgcataa
     1381 ttaaaattat aaagaatcag cgctttatac tcattcaaaa tgcctgattt tacctgttgg
     1441 tctgtgacta cgtccacagg aacatttgct attctaagag caggataaaa ccaacttgcg
     1501 tcaaatgcgt ggattccaga acctgtgtgg gcaatatgt
//
LOCUS       DAORYU010000307         1533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_55623_length_1533_cov_4.302436, whole genome shotgun sequence.
ACCESSION   DAORYU010000307 DAORYU010000000
VERSION     DAORYU010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55623_length_1533_cov_4.302436"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..527)
                     /locus_tag="PLW07_07545"
     CDS             complement(75..527)
                     /locus_tag="PLW07_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50423.1"
                     /translation="MVFLEIRKNYPFLAVDSRFVYLGEGTHVCGINWGMSGNSDANRF
                     KYYAYPDSGKTLNYKLGPLKATQSNKIGVVNWLYAHDGKGEGAGLICPGLLGKGEDFI
                     FINAVPQKKQLKKNNYIEVFFITMPISKNFKILQQIYDEIKTVKWVYE"
     gene            complement(544..849)
                     /locus_tag="PLW07_07550"
     CDS             complement(544..849)
                     /locus_tag="PLW07_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50424.1"
                     /translation="MKRILLMILPVLFFICSGQARDKLYVEVQKEDVLIATESALYNV
                     SADGIYSAGYIMYFGAKDFTANKDLTVKKLKEAKVVVDKPDRKVVKAVFLVEPEWYH"
     gene            complement(887..>1533)
                     /locus_tag="PLW07_07555"
     CDS             complement(887..>1533)
                     /locus_tag="PLW07_07555"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50425.1"
                     /translation="LSMLENRNKFDFVSAKKDLEKVQEIQQVAFKYNPQMFSEKPATS
                     YMKRFFSPCVEQGYERVTGKNIFVAGLPDEWDFLIDPESLGEELGYFRTGKIGGNWTK
                     IKTKTLSWSDQGLRYYKGDAWYRIVVTIPEKFRGKKVLLWFGGVDELAKVWLNGNVIG
                     TSPGKAFVPFEFDVTDFVNWGKENFIAVKITNERLNELGTGGITAPVMFYARQE"
BASE COUNT          441 a          369 c          217 g          506 t
ORIGIN      
        1 ggatttatcc gtagttttgt ctgtgccgca gcacccaagt gaataaagat atcccatttt
       61 ctttcccact catttcactc atacacccac ttcactgtct ttatttcatc gtatatctgc
      121 tgcagaatct tgaaattctt tgaaattggc attgtgataa aaaacacctc aatataattg
      181 ttttttttca attgtttttt ctggggaaca gcattgataa aaatgaaatc ttcccccttg
      241 ccaagcaaac cagggcagat taaacctgct ccttctcctt tcccatcatg ggcatataac
      301 cagtttacca cgccgatttt attgctctgc gtggctttca atggaccaag tttgtaattt
      361 aacgttttcc ctgaatcagg atatgcgtaa tatttaaacc tatttgcatc tgaattaccg
      421 ctcattcccc agttaatacc gcatacatgc gtaccctctc cgagatacac aaaacgggaa
      481 tcaacagcaa gaaatggata attttttctt atttcgagaa aaaccacatg ttcgtaatta
      541 ttatcaatga taccattctg gttctaccag aaaaactgcc tttaccactt ttctgtcagg
      601 tttatccaca acaacctttg cttctttaag tttttttaca gtcaggtcct tgtttgcagt
      661 aaaatctttt gcgccaaaat acattatgta accggctgaa taaataccat ccgcagaaac
      721 attataaagg gcactctctg tcgcaatcaa aacatcttct ttctgaactt caacataaag
      781 cttatccctt gcctggccag agcaaatgaa aaacaataca ggcaaaatca tcaacaaaat
      841 tctcttcatt ttctgcctcc tttccgggat tcccccggat aatattttat tcctgccttg
      901 cataaaacat cacaggagca gtaattccac ctgttccaag ttcgttcaat ctttcatttg
      961 taatttttac cgcaataaag ttttctttgc cccagtttac aaaatctgtc acatcaaatt
     1021 caaaaggaac aaacgctttg ccaggacttg ttccaattac atttccattg agccatacct
     1081 ttgccagttc gtcaactcca ccaaaccaca acaatacttt tttgcccctg aatttttctg
     1141 gaatagtaac gacgattctg taccacgcat cccccttgta gtacctcaat ccctggtcac
     1201 tccaggaaag tgtctttgtt ttgatttttg tccagttacc accaatttta ccagttctga
     1261 aataaccaag ttcttcccct agggattcag ggtcaatcaa aaaatcccat tcatccggta
     1321 atccagccac aaatatattt tttcctgtga ccctttcata cccctgttca acacatggtg
     1381 aaaaaaatct tttcatgtaa gatgtggctg gtttttcaga aaacatctgt gggttatatt
     1441 tgaaagcaac ctgctgaatt tcctggactt tttcaagatc tttttttgcg ctaacaaaat
     1501 caaatttgtt tctgttctca agcatagaca aga
//
LOCUS       DAORYU010000308         1528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_55891_length_1528_cov_2.598778, whole genome shotgun sequence.
ACCESSION   DAORYU010000308 DAORYU010000000
VERSION     DAORYU010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55891_length_1528_cov_2.598778"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /gene="argF"
                     /locus_tag="PLW07_07560"
     CDS             <1..842
                     /gene="argF"
                     /locus_tag="PLW07_07560"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOL50426.1"
                     /translation="FFKYQRKKQIMISDTLKGMCIGLLFRKPSSRTRISFEVAIIELG
                     GNPMFLEAGQLQISRGETIYDTARVLNLYLSGLVIRTYQQSEVETFAKYVDFPVVNAL
                     TDLFHPCQILADFFTIWEVKKNLEDAVITYIGDANNICNTLILGADILGIKINVSCPQ
                     EFSVSEEIIKSMKNPSLLNINNDPSAFISESDVIYTDTWVSMGNEQEKQRRLEIFRPY
                     QVNEKLVQNAREDFIFMHCLPAHRGEEVTDEIIDGKNSVVWQQAENRLHVQKAILHFL
                     YSG"
     gene            853..>1528
                     /locus_tag="PLW07_07565"
     CDS             853..>1528
                     /locus_tag="PLW07_07565"
                     /inference="COORDINATES: protein motif:HMM:NF013486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOL50427.1"
                     /translation="MFSEKRIKKVIAHLESKKIDCFVTANPSNIFYLTGIYNVEGYLC
                     ISKKDMKFFTGGIYYQYAVDRQNVLKIPDFQCEQIKGKNFYAYLKSFKKPSMLSSEIS
                     AGRWEQLCQETGRKINLIDNFIARMRMVKDEEEIMKIKKAGKIAYSVLNKVKRLIKPG
                     VSELDIAAEILYQIRKQGGDKEAFGVIVASGINSSYPHHQPSTRKFKDNDIVVIDMGV
                     CLDGYNS"
BASE COUNT          519 a          259 c          304 g          446 t
ORIGIN      
        1 aattctttaa gtatcagaga aaaaaacaaa tcatgatttc agatacccta aaaggtatgt
       61 gcataggact tctgttccgt aaaccatcaa gcaggacaag aatttctttt gaggtcgcaa
      121 ttattgaact gggtggaaac ccgatgtttc tggaagcagg gcagttacag atttcaagag
      181 gagaaacaat ttatgataca gcaagggttc tcaatttata tttaagcggt cttgtaatca
      241 gaacatacca gcaatcagaa gttgaaacat ttgcaaagta tgttgacttt cctgtggtga
      301 atgcccttac agaccttttt catccatgcc agattcttgc tgactttttt acaatctggg
      361 aggtaaaaaa gaatcttgaa gatgctgtga ttacttatat cggcgatgcc aataatatct
      421 gtaacacctt gattcttggc gcagacattc ttggtatcaa gattaatgtc tcatgtcccc
      481 aggaattttc ggtgagcgaa gaaataatca agtcaatgaa aaatccgtct cttttgaaca
      541 taaacaatga cccttctgcg tttatttctg aatcagatgt gatttataca gatacctggg
      601 tgagtatggg taatgagcag gaaaaacaga gacgccttga aattttcaga ccataccagg
      661 tgaacgaaaa acttgttcaa aatgcacgag aggactttat ttttatgcat tgtctgccag
      721 cccacagagg agaagaagtc acagatgaaa ttatagatgg aaaaaattct gttgtctggc
      781 aacaggcaga aaatcgtctg catgtccaga aagcaatttt acactttctt tacagtggtt
      841 gagccaatct aaatgttttc agagaaacgg ataaaaaaag ttattgcaca tcttgaatca
      901 aaaaaaattg actgttttgt tactgcaaat ccttcaaaca tattttatct cacagggatt
      961 tacaatgttg aaggttatct ttgcatcagc aaaaaagata tgaaattttt taccggtggc
     1021 atttattatc agtatgctgt tgacaggcag aatgttctca agattcctga ttttcaatgt
     1081 gaacaaatca agggtaaaaa cttttacgcc tatttaaaat cattcaagaa accctcaatg
     1141 ctttcatcag aaatttctgc tggtagatgg gaacagttat gccaggaaac aggcagaaaa
     1201 atcaacctga ttgacaattt cattgcccgc atgagaatgg tgaaagatga agaagaaata
     1261 atgaaaataa aaaaagccgg gaaaattgcc tattcggtat taaataaggt aaagcgtctc
     1321 atcaaaccag gcgtttcaga actggacatt gccgctgaaa ttctttatca gatacgaaaa
     1381 cagggtggag ataaggaagc atttggagtg attgttgctt ctggtatcaa ttcatcttat
     1441 ccccatcatc aaccatcaac aagaaaattt aaagataatg acatcgttgt cattgatatg
     1501 ggagtgtgcc ttgatggtta taatagcg
//
LOCUS       DAORYU010000309         1521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_56262_length_1521_cov_3.278990, whole genome shotgun sequence.
ACCESSION   DAORYU010000309 DAORYU010000000
VERSION     DAORYU010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56262_length_1521_cov_3.278990"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..>1521
                     /locus_tag="PLW07_07570"
     CDS             152..>1521
                     /locus_tag="PLW07_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50428.1"
                     /translation="MCRKLFVIVLFLVTLNTILCKAEGVKNIPSIKNTSVSIPVEEFT
                     KLIKDAKISLSWSEFAKLLASEDGTKYVRIPWEIFLKYIEPASVIKPSRPPVDYILSD
                     ITYTGKPEVNSCVLNFSANLLVLKNPSDGWTTVKLWPAYQSVGISEMKCNGKPANIYT
                     GSDGNYQILINEQGNFVLEGRLYIRLGGKGESFTLSSIPNTVSTLKLSLPKDYQVTSS
                     AGIMKINRAKDSTEVELVFSPIGSMDVNWSLMSKEPSEPKIFADVSISSYLQSDMLKT
                     NASLRYDILYQPVKFLKILIPEGMRLTNVSGNIMDWKQIDSVLQVELKPETKGRINIE
                     ISCEQELKQDIAEIDIKSPVITDAEKTTGYFTITTAQNLGVNPVAYEGLLIVDPKEVP
                     AAAAGTVLAYRFNRLPFTTTVKITRYQELPVLEATCDSVNAITATTIDGKTITRVIYH
                     VRNNTR"
BASE COUNT          505 a          292 c          296 g          428 t
ORIGIN      
        1 agcagggtct ggtgcctgcc cataaagacc cgggtaggtt gcttgagtca ggaagtgatt
       61 tctgacccag atggattgcc acctgaaaac agaacccggc ttacgagtta ctccctgaaa
      121 atttctgtag tataacaaat gggaggttta tatgtgcaga aaattgtttg tgattgtttt
      181 gtttctcgta acattgaata ccatactctg caaagcagaa ggagtaaaga atataccttc
      241 aatcaagaat acttcggtct caattcctgt tgaagaattt acaaaactta tcaaggatgc
      301 aaaaatttct ctatcctggt cagaattcgc aaaattgctg gcatctgagg atggtacaaa
      361 atatgtcaga attccatggg aaatctttct gaaatatatt gaaccagcat cagtaataaa
      421 accgtccaga ccaccggttg actacatcct ttctgatatc acatacactg gtaaaccaga
      481 agtcaattca tgcgtattga atttttctgc aaatcttctg gttctaaaga acccttctga
      541 tggatggaca actgtaaaac tctggcctgc gtatcagtcg gttggaattt cggaaatgaa
      601 atgtaatggg aaaccggcaa atatctacac cggttcagat ggtaattatc aaattctaat
      661 caatgaacag ggaaattttg tacttgaagg caggttgtat ataaggttgg gtggaaaagg
      721 agaatctttc acgctttctt ctattccaaa cacagttagc acacttaaat tatccctgcc
      781 aaaggattat caggtgactt cttctgcagg aataatgaaa attaatcgag caaaagattc
      841 cactgaggtt gagcttgttt tttcaccgat tggttcaatg gatgtcaact ggtctcttat
      901 gtcaaaagag ccatctgaac caaaaatttt cgcagatgtt tcaatttcaa gctatcttca
      961 atcagatatg ctcaaaacaa atgcttcatt gagatatgat atcctctatc aacctgtaaa
     1021 attcttaaaa atattgatac ctgaaggtat gcgactcacc aatgtttctg gtaacatcat
     1081 ggactggaaa caaattgatt cagttttgca ggtagaattg aaaccagaaa caaaaggaag
     1141 gataaacata gaaatttcct gtgaacagga gttaaaacaa gacattgctg aaattgatat
     1201 caagagccct gtcattactg atgcagaaaa aaccacagga tactttacca ttacaacggc
     1261 tcaaaatctc ggcgtcaatc cagttgcata cgaaggtctt ctaatcgtag acccaaagga
     1321 agttcctgca gcagcggctg gaacagtcct ggcatacaga ttcaatcgtc ttccatttac
     1381 aacgactgtg aaaattacaa gatatcagga attgcctgtt cttgaagcaa cctgtgattc
     1441 tgtaaatgct attactgcga caaccattga tggaaaaacg attacacggg taatttacca
     1501 tgtgagaaac aacacaagac a
//
LOCUS       DAORYU010000310         1516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_56484_length_1516_cov_4.982204, whole genome shotgun sequence.
ACCESSION   DAORYU010000310 DAORYU010000000
VERSION     DAORYU010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56484_length_1516_cov_4.982204"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..743
                     /locus_tag="PLW07_07575"
     CDS             165..743
                     /locus_tag="PLW07_07575"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HOL50429.1"
                     /translation="MIVPAALSLWVLWKIFVFLESLIGQWIQKAIPQFYVRGLGFVSL
                     IAIILILGFFAQNIFGRRIIRYLEKGFLSIPVFNKLYQFVHSILQQLFRKEKEIFHGV
                     ALVQLSNDVSTIGFITSKEPVVSGLNNDYWTVFIPTVPNPTSGFVLFVHKDNLKPLPI
                     GVEQGMKILLSFGIYKISDAANQNKSTDSQKN"
     gene            700..1407
                     /gene="recO"
                     /locus_tag="PLW07_07580"
     CDS             700..1407
                     /gene="recO"
                     /locus_tag="PLW07_07580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOL50430.1"
                     /translation="MPPTRIKALILKRINFRETSVIVHLLTDSLGKISGLMKGVRNPS
                     KKIPPLAYQQGACIECFVYLKQKPGLELVTKPEIIESFNLQQQDSVLWKKLLLTADKF
                     IPASRFNSKQLFDLLFATACLIPKAKNQRPVEIFVTERVLFLLGFGPFLEKCLVCGAE
                     KQLNFFSGKLGGVVCTRCQAGEPTAFRLPEKHIQVLRFFRKIPVNQISMVKYIPDELF
                     TNLKKCLDEIVRYHITD"
BASE COUNT          461 a          264 c          300 g          491 t
ORIGIN      
        1 gtatgggttg ctcagggaag gattatcaag cgaattcata caattgaaga aaccgtgatt
       61 ggcgcaattt ttggcatact gattacggtg ctattgattc aacttgtgga aaaatttaaa
      121 ataattccat gaaactcaga aactatttca tatctggtgt gctggtgatt gttcccgcag
      181 cactgtcttt gtgggtgttg tggaagattt ttgtttttct tgaaagtttg attggtcagt
      241 ggattcaaaa ggcgattccg cagttttatg tcaggggatt ggggtttgtg tcactgatag
      301 caattatctt gatactgggt ttttttgctc agaatatttt tggcaggcga atcatccgtt
      361 atctggagaa aggttttctc tctatccctg ttttcaacaa actttatcaa ttcgttcatt
      421 caatcttgca acaactgttc agaaaggaaa aggaaatttt tcatggtgtt gccctggtac
      481 aactttctaa tgatgtttca acaattggtt tcattacgag taaagaacct gtcgtcagtg
      541 gattgaacaa tgactactgg actgttttta ttccaacagt gccaaatcca acatcagggt
      601 ttgttctgtt tgtgcataaa gacaacctga aaccattgcc tataggtgtt gagcagggga
      661 tgaaaatttt gctttccttt ggtatttata aaatttccga tgccgccaac cagaataaaa
      721 gcactgattc tcaaaagaat taatttcaga gaaacaagtg tcattgttca tcttctcaca
      781 gattcactcg ggaaaatctc gggcttgatg aagggtgtaa gaaacccttc aaaaaaaata
      841 ccacctctcg cctatcaaca gggcgcttgc attgaatgct ttgtatattt aaaacagaag
      901 ccaggacttg aacttgtcac aaaaccagaa attattgaat cgtttaatct tcaacagcag
      961 gattctgttc tttggaagaa acttcttctt actgctgata aatttattcc tgcttcaaga
     1021 ttcaacagta aacaattgtt tgaccttctt ttcgcaactg cctgtttgat tccgaaagca
     1081 aaaaatcaaa gacctgttga aatttttgta acagaaagag tactgtttct tctcggattt
     1141 ggaccatttc ttgaaaagtg cctcgtatgt ggcgcagaaa aacaactgaa ttttttcagt
     1201 ggtaaactgg gtggagtagt gtgcacacga tgccaggcag gtgagccgac tgctttcaga
     1261 ttgccagaaa aacacattca ggtattgaga tttttccgta aaatccctgt aaatcaaatt
     1321 tcaatggtaa aatacatacc agatgaactt tttacaaact taaaaaagtg tcttgatgaa
     1381 attgttcgtt atcacataac cgattgaatt atgagaaata tcactgaaaa aaatatcatt
     1441 gacagagttt attcagccat cgaaaaagca agtttttctc ttgaacctga tataatggga
     1501 ttgctgaaaa atgctg
//
LOCUS       DAORYU010000311         1516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_56485_length_1516_cov_4.769336, whole genome shotgun sequence.
ACCESSION   DAORYU010000311 DAORYU010000000
VERSION     DAORYU010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56485_length_1516_cov_4.769336"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="PLW07_07585"
     CDS             complement(<1..416)
                     /locus_tag="PLW07_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOL50431.1"
                     /translation="MEWINSFIFTSREPPADAETISQKLMHQAGMIDKLVSGIYSYLP
                     LGLKVLQKVSNIIREEMNNAGAVEILMPALQPSSLWKESKRFGKMGKDMIVFKDRHDR
                     EMLLGPTHEEVVSDIVRKYVRSWKNLPVMLYQIQTK"
     gene            complement(480..>1516)
                     /locus_tag="PLW07_07590"
     CDS             complement(480..>1516)
                     /locus_tag="PLW07_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50432.1"
                     /translation="LCGIVFGLAALAKPTILAFIPFIIVGLLLWPEKILIDHQKTTKK
                     SFQITMAILFAGLACTLIILPASLRNLAISGKFVLICTNGPVNWQIGNSSDSTGLFYY
                     PSGHLLHPASMDFWKLLIKKTLLFFNNYEWPQNLSIYIARELIPALKFAFVKFGLIVP
                     LGVVGLFLAARKKKNFLFVSFTIVQIMWVIMFFITERYRFPAVACLTVCAGFLITQVI
                     DEIRTKKIVLPFVKLAIAGLWGYIFGWTPGQLFNDMYWKIFAKLSKTNIVYNLQTNNN
                     RLAEKIASDYKKLLPQDPDSHFFLACVYAQKGIMDKAEQELTRTLDLNPEHELARKFL
                     DEIRKNHLSH"
BASE COUNT          442 a          298 c          284 g          492 t
ORIGIN      
        1 tatttcgttt gtatctgata gagcatcaca ggaagatttt tccagcttct tacatatttt
       61 cttacaatat cagaaacaac ctcttcatgg gttggaccca gtaacatttc tctatcgtgt
      121 ctatctttga aaacaatcat atctttaccc atttttccga atctttttga ttctttccac
      181 agagacgaag gttgcagtgc tggcatcagt atttcaacgg ctcctgcgtt gttcatttct
      241 tctcttatga tatttgaaac cttctgtaat acctttaatc caagtggaag atacgaataa
      301 atgccggata ccagtttgtc tatcattcct gcctgatgca taagtttctg gcttattgtt
      361 tcagcatctg ccgggggttc ccttgatgta aaaatgaaag aatttatcca ctccattggt
      421 ttctcctttt cagtcatgat tctttcgaac aaaatattat accgtattat tttgtgatat
      481 caatgagaaa ggtgattttt tcttatttca tcaaggaact ttctcgcaag ttcatgttcc
      541 gggttcagat caagagtgcg cgtcaattcc tgttctgctt tatccataat tcctttctgc
      601 gcgtacacgc aggcgaggaa aaagtgactg tccgggtcct ggggcaacag ttttttgtaa
      661 tctgatgcta ttttttcagc cagccgattg ttgtttgttt gcaggttata cacgatgttt
      721 gttttgctga gttttgcaaa tattttccag tacatgtcgt taaacagttg tccgggtgtc
      781 cagccaaata tatatcccca cagacctgcg atggcaagtt taacaaaagg caatacaatt
      841 ttttttgttc ttatttcatc tattacctgg gtaatgagaa aacccgcaca aacagtaagg
      901 caggcaacag cagggaacct gtaccgttcc gtgatgaaaa acatgatgac ccacataatc
      961 tggacgattg taaatgaaac aaaaagaaaa ttttttttct ttcttgctgc aagaaaaagt
     1021 cccacaactc cgagcggaac aatcagtcca aattttacaa atgcaaattt taatgccggt
     1081 atcagttctc ttgcgatgta tatgctcaaa ttctgtggcc attcataatt gttgaagaaa
     1141 agaagcgttt ttttgataag caatttccag aaatccatac ttgcaggatg caacaaatgc
     1201 cctgatggat aatagaaaag cccggtggaa tctgaactat tacctatctg ccagtttaca
     1261 gggccattgg tgcaaatgag cacaaattta ccgctgatcg caagatttct taaggatgca
     1321 gggagtatta tcaaagtaca cgccagtcct gcaaaaagta tcgccattgt aatctgaaag
     1381 gattttttag ttgttttttg atgatcaatt aggatttttt caggccataa caatagtcca
     1441 acaatgatga aaggaataaa ggcaagaatt gttggttttg caagagcagc aagcccaaaa
     1501 acaataccac atagaa
//
LOCUS       DAORYU010000312         1510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_56808_length_1510_cov_4.912027, whole genome shotgun sequence.
ACCESSION   DAORYU010000312 DAORYU010000000
VERSION     DAORYU010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56808_length_1510_cov_4.912027"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="PLW07_07595"
     CDS             complement(<1..775)
                     /locus_tag="PLW07_07595"
                     /inference="COORDINATES: protein motif:HMM:NF014605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOL50433.1"
                     /translation="MSKEIVLTNQEAQIIFGRHDEHLRLLEDLLGVEIFARGNVVKLR
                     GEPDNIEKAYKTFENLRHKVHRMKYLSPADIFDAMPDEKEKQAQKVEDEHQKHFTSDD
                     IIIIRSKKEAIFPRSAHQRSYIKAIRKYPLTIGIGPAGTGKTYLAVALAIESLRNGLF
                     SKVVLTRPALEAGERLGFLPGDLEEKIKPYLQPIYDALFDLLKYEELKRWSERKIIEI
                     VPLAYMRGRTLNDAFIILDEAQNTTGEQMKMFLTRLGTNS"
     gene            complement(783..>1510)
                     /locus_tag="PLW07_07600"
     CDS             complement(783..>1510)
                     /locus_tag="PLW07_07600"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOL50434.1"
                     /translation="PTKPPEPAFKPSPSTRKTPRSEKQEVEIQIMQAKALIETAMEIS
                     KNAEKYAQESYNTSNQANATAEKALEASNKAIQSSAEAVKAANEAAEKAIAAANEASE
                     KAIAAANKAAKEAMEYADQAAQKSIDAANKAIEAANAAAEKAIIASNKALAACDQVMA
                     ELGRVKATIKAKEIEPVLPEEPKVKISTTGKTYTVKKGDTLSILAKKYYGESGQWRKI
                     YEANRTKIKNPNVLIPGTELVIP"
BASE COUNT          338 a          346 c          296 g          530 t
ORIGIN      
        1 ttgaatttgt gccaagacgc gtaagaaaca ttttcatctg ttcgcctgtt gtattttgtg
       61 cctcatcaag aataatgaac gcatcgttca gtgtcctacc cctcatatag gcaagtggca
      121 caatttctat aatttttctt tcactccaac gtttcaattc ttcatacttc aaaagatcaa
      181 acaatgcgtc gtatattggt tgaagatatg gcttgatttt ttcctcaaga tctcctggca
      241 gaaaacccaa cctttctcct gcttcaagag caggacgtgt cagcacaact ttgctgaaaa
      301 gtccgttgcg caatgattca atcgcaagcg caacagcaag atacgttttt ccagttccag
      361 caggtccaat gccaatagtt aatggatatt ttctgattgc cttgatgtaa gaacgttgat
      421 gtgcacttct gggaaaaatc gcttcctttt ttgaccttat aataatgata tcgtctgatg
      481 taaaatgctt ctggtgttca tcctcaactt tttgagcctg tttttctttc tcatcaggca
      541 tcgcatcaaa aatgtctgca ggagaaagat atttcattct gtgcacttta tgacgaaggt
      601 tttcaaatgt tttatacgcc ttctcaatat tatcaggctc gcctcttaat ttgacgacat
      661 ttccacgagc aaaaatttcc acaccaagca gatcctcgag caatcttaaa tgctcgtcgt
      721 gcctcccgaa tattatctgg gcttcctggt tggttaacac aatctctttt gacaaggaca
      781 tttcagggga taacaagttc tgttcctgga atcaggacat ttggattttt aatctttgtc
      841 ctgtttgcct catagatctt tctccactgg ccagattctc catagtactt tttcgccaga
      901 atagaaaggg tgtctccttt tttcactgta tatgttttac cagtggttga aatttttact
      961 tttggttctt caggaagaac tggttcaatt tcttttgcct ttatcgttgc cttgaccctt
     1021 ccaagctcag ccatcacctg gtcgcaggca gcaagggctt tgtttgaagc aataatcgcc
     1081 ttttctgcag cagcgtttgc tgcctcaatt gccttgtttg ctgcatcaat ggatttctga
     1141 gctgcctgat ctgcatactc catcgcctct tttgctgcct tgtttgccgc tgcaatcgcc
     1201 ttttcagacg cttcattggc agcagcaatt gctttttcag ctgcttcgtt ggcggctttt
     1261 acggcttcag cagatgactg aattgcttta ttagacgctt ccagcgcctt ttcagctgtg
     1321 gcattcgcct ggttgctggt attgtaggat tcctgagcat atttctctgc gtttttgcta
     1381 atttccatgg ctgtctctat caatgccttt gcctgcataa tttgaatttc cacctcctgt
     1441 ttttcagaac gaggagtttt ccgagtgctg ggcgatggtt taaaagccgg ttcaggcggt
     1501 tttgttggtt
//
LOCUS       DAORYU010000313         1507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_56969_length_1507_cov_3.651515, whole genome shotgun sequence.
ACCESSION   DAORYU010000313 DAORYU010000000
VERSION     DAORYU010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56969_length_1507_cov_3.651515"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..>1507
                     /gene="purH"
                     /locus_tag="PLW07_07605"
     CDS             140..>1507
                     /gene="purH"
                     /locus_tag="PLW07_07605"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOL50435.1"
                     /translation="MIKVKRALISVSDKTGLVDLAKVLVKNGVEIVSTGGTAKTLKGA
                     GIAVKEISQLTGFPEILDGRVKTLHPAIYGGILYKRDNPSHIEQVSQHNIGQIDLVVV
                     NLYPFEKVFFSENATDEELIENIDIGGPSMIRASAKNWKFVCVVVNPSFYPLIAQEIE
                     NGGISEETSRKCAAEVFHTTSYYDWLISRYFDKNKQPSFEDTIGFYYKKIDQLRYGEN
                     PHQQASWYRYADKQIQWKQLQGKQLSFNNILDMESAYRIACEFEEPSCCIIKHNNPCG
                     AAKGKNIKEAFEKALRTDSLSAFGGIIGINKDLDVATATLIKEIFFEVVIAPSFSKEA
                     IDILSQKENLRLIEMPHLPMGEFEIKNAPGGILLQTYDKGNWDTLEVKTKKKPADEEL
                     EALKFAMIICKYVRSNAIVLATTNQIVGVGAGQMSRYDSTRVAVMKMQDNFKEKISPL
                     VMASDA"
BASE COUNT          518 a          263 c          318 g          408 t
ORIGIN      
        1 gacctttaca ttacttatta tctttctgat ccaagaaatt ttgcctgccg aaaatcattg
       61 tttgttcagg aaatcccgga taatctggtt gaacaatggt catttgacta acttttttct
      121 tctaacaggg ggggttctca tgattaaggt taaaagagca ttgataagcg tttctgacaa
      181 aacaggcctt gttgatcttg cgaaagtgct ggtgaaaaat ggagtggaaa ttgtttccac
      241 cgggggaaca gcaaaaacac tgaaaggagc cggaattgct gtaaaggaaa tttcacaatt
      301 gacagggttt ccagaaattc ttgacggcag ggtcaaaaca ctgcatcctg ccatttatgg
      361 aggtattctt tataaaagag acaatccttc tcacattgaa caggtttcac aacacaatat
      421 aggacagatt gaccttgttg ttgttaatct gtatccattt gaaaaggtgt tcttttctga
      481 gaatgcaaca gacgaagaac tgatagaaaa tatagacatc ggtggccctt caatgatacg
      541 ggcatcagca aaaaactgga agtttgtatg tgtggttgtt aacccgtcat tctatccatt
      601 aattgcgcag gaaatagaaa atgggggaat ttccgaagaa acatcaagaa aatgcgcagc
      661 agaggttttt cataccacaa gttattatga ctggcttatc agcagatatt ttgataaaaa
      721 caaacaacct tcttttgaag ataccattgg attttattat aaaaagattg accagctcag
      781 atacggtgaa aaccctcatc aacaggcatc atggtatcga tacgctgaca aacaaattca
      841 atggaaacag ttgcagggga aacaactttc ttttaataat attcttgata tggaaagtgc
      901 ttatagaata gcgtgcgagt tcgaagaacc atcatgctgt attatcaaac acaacaatcc
      961 ctgcggtgca gcaaaaggga aaaacatcaa agaagcattt gaaaaagcgt tgagaacaga
     1021 ttcattgagt gcctttggcg gtattattgg gattaacaaa gaccttgatg tagcaacagc
     1081 aacattgatt aaagaaattt tctttgaagt cgtaatagcc ccttcatttt caaaagaagc
     1141 aattgacatt ctttcacaga aggaaaacct cagattgatt gaaatgccgc atctccccat
     1201 gggtgagttt gaaattaaaa atgcgcctgg tggaatattg cttcagacat acgacaaagg
     1261 aaactgggac acgcttgaag tgaaaacaaa aaagaaacca gcagatgagg aacttgaagc
     1321 actaaaattt gccatgatta tatgcaagta tgtcagatca aatgctatag tgctggcaac
     1381 aacaaatcaa attgtgggcg ttggcgcagg acagatgtca agatacgact caacaagggt
     1441 tgcagtgatg aaaatgcagg ataatttcaa agaaaaaatt tctccccttg tgatggcttc
     1501 agatgca
//
LOCUS       DAORYU010000314         1492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_57811_length_1492_cov_5.480863, whole genome shotgun sequence.
ACCESSION   DAORYU010000314 DAORYU010000000
VERSION     DAORYU010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57811_length_1492_cov_5.480863"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="PLW07_07610"
     CDS             <1..277
                     /locus_tag="PLW07_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HOL50436.1"
                     /translation="KKAGALIVGTGRSDYPNQVNNLLAFPGIFRGALDARAKQITERM
                     KTEAAKAIAGIIPENKLNPDYVIPSPFNKQVALAVAEAVKRVAKKSG"
     gene            280..693
                     /locus_tag="PLW07_07615"
     CDS             280..693
                     /locus_tag="PLW07_07615"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOL50437.1"
                     /translation="MVFLILCIIFYSGAALLYRHANHLHCNRLAIIVSERISAVILMS
                     LYMLFFDTFNITSGATLLAFAGGITIFISRLALLTSLKYGKISSSWTAVNLSLVIPIL
                     ASMIFWKEIPNLRQTIGLLMVPLAIILLQEEPGEK"
     gene            697..1104
                     /locus_tag="PLW07_07620"
     CDS             697..1104
                     /locus_tag="PLW07_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50438.1"
                     /translation="MWIKFILISFFLAGISDTTWKMAGETCGASVNSYLLLFHVFALL
                     SATFAVIKTRTGIKKIEFLLGSIIGFALISGGIASMQAILRIPGIIYFPVASCGNLLI
                     VTIMANILWKEKPTTRQIAGLVIACISIVLIAL"
     gene            complement(1091..>1492)
                     /locus_tag="PLW07_07625"
     CDS             complement(1091..>1492)
                     /locus_tag="PLW07_07625"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOL50439.1"
                     /translation="RNPHLVSCCLALCPATDMKKLYYFWHGTERDFLAKTIENAYGGT
                     PEKNAAQYKERSSIYLVEKLCSMPVAIIHGDSDIVIPVEHSRRFIEKSRKAGVKIYYH
                     EIKDGNHDSPIMDFAIVKKAIDWLEFAITGQ"
BASE COUNT          435 a          295 c          287 g          475 t
ORIGIN      
        1 aaaaaaggca ggcgcattga ttgtgggaac aggaagaagc gattatccca atcaggtcaa
       61 taatcttctg gcatttcctg gaattttcag gggcgctctt gatgcgagag caaaacaaat
      121 cactgaaaga atgaaaacag aagcagcaaa ggcaattgct ggaataattc ctgaaaacaa
      181 attgaatcct gattatgtca taccatctcc atttaataaa caagtcgcgc ttgccgtagc
      241 agaagcagta aaaagagttg ccaaaaaatc tggataaaga tggtctttct gatactctgc
      301 attatctttt attcaggcgc tgcactgtta tacaggcatg caaatcacct tcattgcaat
      361 agattggcta ttattgtttc tgaaagaatt tcagcagtca ttcttatgtc gctttatatg
      421 ttgttttttg acactttcaa catcacatct ggtgcaacat tacttgcgtt tgctggcgga
      481 attacgattt tcatttcgcg gctggcatta ctgacatcat taaaatatgg aaaaatttcc
      541 agttcctgga cagctgtaaa tctatctctc gttataccaa tacttgcttc aatgattttc
      601 tggaaggaaa tacccaatct tcgccagaca atagggctgt taatggtgcc attggcaatc
      661 atactattgc aggaagaacc aggagaaaaa taagttatgt ggataaagtt tattctgata
      721 agtttttttc ttgctggtat ttcagacact acatggaaaa tggcaggaga aacctgcggc
      781 gcttctgtta attcttatct actactgttt catgttttcg ccctgctgag cgccactttt
      841 gcagtcatta aaaccagaac aggaataaaa aaaattgagt ttttacttgg ctcaatcata
      901 ggatttgctc tgatttctgg tggaattgcc tcaatgcagg caatattgag aattccgggt
      961 attatttatt ttcctgttgc cagctgcgga aacttactga ttgttacgat catggcaaac
     1021 atcctctgga aggaaaaacc aacaacccgg cagattgcag ggcttgtaat tgcctgtatt
     1081 tctattgtgc ttattgccct gtaatggcaa actctaacca gtcgatcgcc ttttttacaa
     1141 tcgcaaagtc cattattgga gaatcgtggt tgccatcctt tatctcatga taataaattt
     1201 ttacacccgc ctttctggat ttttcgataa atcttcttga atgctcaaca ggaattacaa
     1261 tatcactgtc tccatgaatg attgccactg gcattgaaca aagtttttct accagataaa
     1321 tacttgacct ttctttatac tgtgcagcgt ttttttcagg tgtcccgcca taggcatttt
     1381 ctattgtttt tgcaagaaaa tccctttcag tgccatgcca gaaataataa agttttttca
     1441 tatctgtggc aggacatagc gccagacaac aggaaacaag gtgcggattt ct
//
LOCUS       DAORYU010000315         1490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_57925_length_1490_cov_4.304530, whole genome shotgun sequence.
ACCESSION   DAORYU010000315 DAORYU010000000
VERSION     DAORYU010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57925_length_1490_cov_4.304530"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1490)
                     /locus_tag="PLW07_07630"
     CDS             complement(<1..>1490)
                     /locus_tag="PLW07_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50440.1"
                     /translation="KWDIFDIDGNAVYQGKQKVVFEDVKTETLFVPLRVKENGWYRIK
                     ISLLDRQNRLLIEHPASFAILAEDKRQYGYESPFGTWWFGGAHFGTSDSNIVGPLLLK
                     AGLRHSVFSQAWHGKLTEKELSQWKVTSFQVPWNWSMKEEIEKQAKNYENHVKEYLEK
                     WRNCKYALIFHESYGGDTIPPELYGENPKNTDEKKNKEIEKLMMIASSATRVLREKFP
                     DVKIVFGNCNSVSDLVAEFFRRGYPVENIDYFGIESAGQTWMPEKPIEYGTQAAWFVR
                     ETGRKFGYNLPVTSCYEWIYRQDRLLGEKTQAEWYVRDALIGLAYRFPTISIALLYDV
                     GNCYYNTLWGASGLLKRYPLLYPKPSYVAMSTLTRVLDRAEFLRRVRTSSDTVYALEF
                     KKGQDYVYVVWTPRGRCEVDLQFENEGSISIEDIFGRNKPFSLNKRTLKFVAGTDVTY
                     IVSSVPVKEIVSGKRIFDQSNESRKATIVNRMDSILEWELIREKDT"
BASE COUNT          411 a          348 c          236 g          495 t
ORIGIN      
        1 cgtgtctttt tctctaatca gttcccattc taagatgcta tccatcctat tgactatcgt
       61 tgcttttctt gactcattgc tctggtcgaa aattcttttt ccggagacaa tttccttaac
      121 aggtacactg ctgacaatgt aagtcacatc tgtacctgcc acaaatttta atgtcctttt
      181 gttcaacgaa aatggtttgt tccgaccgaa tatgtcttca atactgatac ttccttcatt
      241 ttcaaattgc aaatcaactt cacatctacc tcttggtgtc cagacaacat atacataatc
      301 ctgccccttt ttgaattcaa gagcataaac ggtatcagaa gaagttcgaa cccttctcaa
      361 aaactcagct ctgtcaagaa cccttgtaag agttgacatg gcaacataag atggttttgg
      421 ataaagtaga ggataccttt ttaatagccc ggaagcgccc cacagagtgt tataataaca
      481 attcccaaca tcataaagaa gcgctattga gattgttggg aacctatagg caagaccaat
      541 caaagcatct ctcacatacc attcggcctg ggttttttct cccaaaagcc tatcctgccg
      601 ataaatccac tcataacatg atgttacagg tagattatag ccaaattttc tgccagtttc
      661 tctaacaaac catgcggcct gcgtaccata ttcaatgggt ttttctggca tccacgtttg
      721 gccggctgac tctataccaa agtaatctat gttttccact ggataccctc ttctaaaaaa
      781 ttctgctacc aggtctgaaa ccgagttgca gttaccgaaa acaattttta cgtcagggaa
      841 tttttctctt aacactctgg ttgcagaaga agcaatcatc ataagttttt cgatttcctt
      901 atttttcttc tcatctgtat tttttgggtt ttcgccgtat aattctggtg gaatggtatc
      961 gccaccataa ctttcatgaa aaattagagc gtatttacaa tttcgccatt tttctaaata
     1021 ttccttcacg tgattttcgt aattttttgc ctgtttttca atttcttctt tcatcgacca
     1081 gttccacggc acctgaaaac tggttacctt ccattgagaa agctcttttt ctgtcaattt
     1141 tccatgccag gcctgactga aaacagaatg tctgagccct gctttcaata aaagtggccc
     1201 tacaatgttt gagtctgatg ttccaaaatg cgcgcctcca aaccaccatg ttccaaaagg
     1261 tgattcgtat ccatattgcc tcttatcttc ggcgagtatt gcgaaagatg caggatgttc
     1321 aatcaaaaga cgattttgcc tgtccaataa agagattttg atgcgatacc agccattttc
     1381 tttcaccctc aatgggacaa acaaagtttc tgtttttacg tcctcaaaca ctactttctg
     1441 ttttccttga tacacagcat ttccatcaat atcaaaaata tcccatttta
//
LOCUS       DAORYU010000316         1469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_59186_length_1469_cov_4.465347, whole genome shotgun sequence.
ACCESSION   DAORYU010000316 DAORYU010000000
VERSION     DAORYU010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59186_length_1469_cov_4.465347"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(342..>1469)
                     /locus_tag="PLW07_07635"
     CDS             complement(342..>1469)
                     /locus_tag="PLW07_07635"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOL50441.1"
                     /translation="IQFMPLNTLFQLYATKQECPWLVSSSYKMIFMPDLFNYWLCGTI
                     ANEFTIASTSQMLNPSEKGYKPRGNWAKDILKKLGIPTDILCEIVPPGKKLGYFSKYV
                     REETGCDKIPVYSVCCHDTASAVFAVPAEGNWAYLSSGTWSLLGRELDNPLINQQVLE
                     YNFTNEGGYNGKIRFLKNIMGLWIWQECRRQWEKTHNRQFSYVELGELASKEKPFGSL
                     IDVNNSMFLFPGNMVEKVQKFCKTTNQKIPVSIGEISRTILESLALEYRLHIERLEAI
                     AGRIDTLHIVGGGSQNKVLSSFTASSVNKRVLCGPVEATSIGNIGVQMIAAKVVKNHT
                     EFRKLVAKSFPVIEYLPENHDDWQQSYEKYLQLKKISDQQI"
BASE COUNT          450 a          307 c          298 g          414 t
ORIGIN      
        1 caaagagggg ctgggatgga tttgatgaaa tcgaaaaata cttaatgaaa tcccccttga
       61 tccccctttt acaaaggggg aaaaccatga gaggaatttg aaaaggggga agaccgggag
      121 aggaatttgc aaggggaaaa cagtaggagg aatttgaaaa ggggggaaat gggcggggac
      181 gaaaaggggc tgggatggat ttgatgaaat ccctcttgca tctccctttt ataaagggag
      241 aaaatggagc aggaatttgt aaatcaaaaa tactgaaaaa gtcaaaaacc tttccgtttg
      301 tactttgcac aaagacaatt accaatagcc cgcagaagac atcaaatttg ctggtcagat
      361 atctttttca attgcagata tttttcatac gactgttgcc agtcatcgtg attttccggc
      421 agatactcaa taacagggaa tgacttcgcc acaagtttcc tgaattctgt gtgatttttc
      481 actacctttg ccgctatcat ctgaactcca atatttccaa tggatgtcgc ctccacaggt
      541 ccacacaaca cccttttatt gacgctggac gccgtgaaag aagaaagcac cttattctga
      601 gagccaccgc caacaatgtg aagcgtgtca attcgccctg caatggcttc gagtcgctca
      661 atatgtaatc tgtattcaag agccagactc tcaagaattg tccttgaaat ttcaccgatg
      721 ctaacaggaa ttttttgatt cgttgtttta caaaactttt gaactttttc aaccatgttg
      781 ccggggaata gaaacatgga attgttgaca tcaatcagcg aaccaaacgg tttttctttt
      841 gaggcaagtt ctcccagttc tacataggaa aactgtcggt tatgagtttt ttcccactgt
      901 cgcctgcatt cctgccatat ccagagtccc atgatgtttt tcaaaaatct gattttcccg
      961 ttataaccac cttcgttggt aaaattatat tcaagcacct gttgattaat cagcgggtta
     1021 tcaagttccc tcccaagaag agaccatgtt cctgaagaaa gatatgccca gttaccttct
     1081 gcagggacag caaacacagc agacgcagta tcgtgacagc acacagagta tactggaatc
     1141 ttgtcgcaac cggtctcctc gcgcacatat tttgaaaaat atcccagttt tttaccaggt
     1201 ggaacaatct cacaaaggat atctgttgga ataccaagct ttttcagaat atcctttgcc
     1261 cagttacccc ttggcttata tcctttttca gacggattta acatttgaga cgttgaagca
     1321 attgtaaact catttgcaat agtaccacac aaccagtaat taaataagtc aggcataaaa
     1381 atcattttat atgacgatga aactaaccag ggacattcct gttttgttgc gtacaactga
     1441 aataatgtgt taagcggcat aaattgaat
//
LOCUS       DAORYU010000317         1468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_59243_length_1468_cov_3.896674, whole genome shotgun sequence.
ACCESSION   DAORYU010000317 DAORYU010000000
VERSION     DAORYU010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59243_length_1468_cov_3.896674"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="PLW07_07640"
     CDS             <1..322
                     /locus_tag="PLW07_07640"
                     /inference="COORDINATES: protein motif:HMM:NF025179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HOL50442.1"
                     /translation="VLLNAEIRDLQKELDRLTSYQTQLTQILRELKAQESLYSFLTAQ
                     LESAKLEETKDTPFIQVLDTAVVPQKVYSPDIKKILIIIIGIIVGVGIIVLFFDVLRF
                     LGSI"
     gene            327..>1468
                     /gene="dnaX"
                     /locus_tag="PLW07_07645"
     CDS             327..>1468
                     /gene="dnaX"
                     /locus_tag="PLW07_07645"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOL50443.1"
                     /translation="MEKEVYQVLARKYRPRTFAEIAGQQTIVKKLQQAITSKRIPHAF
                     LFAGPRGVGKTTIARIFARALNCVEGPAVEPCNRCVFCMEIYQGTSMDFLEIDGASNR
                     GIEEIRSLREAVSLRPAKARFRIYLIDEVHMLTNEAFNALLKTLEEPPDYAKFIFATT
                     SPEKIPATIVSRCQRYDFKMLNDEEISMMVKKVVENEGYTIEPDAMKKLIEAGTGSMR
                     DILGYLDQIIVLSNTSNITQELVSEVLGITSGESVWELLKLIVSGDVKQSILYLHRII
                     GQGKDITNLLGVMAKKIQLVIWNQYGIVTSDEEIDKGNIELFSRVPAEKFIRALESIM
                     QTKEKIRYEPLELILAELLCFNLVEILKTPGEGSAELFAEKGEKNVV"
BASE COUNT          467 a          267 c          345 g          389 t
ORIGIN      
        1 tgtgctgctc aacgcagaaa taagagacct gcaaaaagaa cttgaccggc tgacgagtta
       61 tcagacacaa cttacacaga ttctcaggga actgaaggcg caggaaagtt tgtattcatt
      121 cctgacggca caacttgaaa gtgcaaaact ggaggagacg aaagacactc catttattca
      181 ggttcttgac acagctgttg ttccccagaa ggtttatagc cctgacatca aaaaaattct
      241 tattattatc attggaatta ttgtcggggt gggtatcatt gttctatttt tcgatgtgtt
      301 aagattcctt ggaagcatat aaagacatgg aaaaagaagt gtatcaggtt cttgcacgca
      361 agtacagacc ccgtacattt gcagaaattg cagggcagca gaccattgta aaaaaactcc
      421 agcaggcaat tacgagcaag agaattcctc atgctttttt gtttgctgga ccaaggggtg
      481 tgggaaaaac aacgattgcc cgcatttttg caagggcgtt aaactgtgtg gaaggacccg
      541 ctgtggaacc atgcaacaga tgtgttttct gcatggaaat ttaccagggc acttccatgg
      601 actttcttga aatagatgga gcatcaaatc gcgggataga agaaattcgt tcactcaggg
      661 aagcagtttc attaagaccc gcaaaggcga gattcagaat atacctgatc gatgaggttc
      721 acatgcttac caatgaggca ttcaatgccc tgctgaaaac cctggaagaa ccacctgatt
      781 acgcaaagtt tatatttgca acgacatcgc ctgaaaaaat tcctgccacg attgtttcca
      841 gatgccagcg ttatgacttt aagatgttaa atgacgaaga aatatccatg atggtgaaga
      901 aagttgttga aaatgagggc tataccattg aacctgatgc aatgaaaaaa ttaattgaag
      961 caggaacagg ttctatgaga gatattcttg gatatctgga tcagattatt gttctgtcaa
     1021 ataccagcaa tataacgcag gaacttgttt cagaggtgct cgggattact tctggtgaga
     1081 gtgtatggga actgcttaaa cttattgtca gcggggatgt gaaacaatcc atcctttacc
     1141 ttcacaggat tattgggcag ggcaaggata ttacaaacct tctcggcgta atggcgaaaa
     1201 aaattcaact ggtgatatgg aatcagtatg gcattgttac atctgatgag gaaatagata
     1261 agggaaacat tgaattgttc agccgcgttc ctgctgagaa gtttatcagg gcgcttgaat
     1321 caataatgca aacaaaagaa aaaatcaggt atgaaccgct ggaactgatt ctggcggagc
     1381 ttttgtgttt taaccttgtg gaaattctga aaactccagg tgaagggtca gcagaacttt
     1441 ttgccgaaaa aggcgaaaaa aatgttgt
//
LOCUS       DAORYU010000318         1466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_59377_length_1466_cov_3.871722, whole genome shotgun sequence.
ACCESSION   DAORYU010000318 DAORYU010000000
VERSION     DAORYU010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59377_length_1466_cov_3.871722"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1275)
                     /gene="malQ"
                     /locus_tag="PLW07_07650"
     CDS             complement(<1..1275)
                     /gene="malQ"
                     /locus_tag="PLW07_07650"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOL50444.1"
                     /translation="MGTGEFLLSTSSGKKWEKIGTLRRAGILTPLFSIHSSSSVGTGD
                     FHTLKCMADLCKKSGDTILQILPLNYPGADNCPYNAISSFAIDPVYISLEDFSYNNFG
                     FSRHLIGDLHVNFLPGKDHRCNYTARDIKLNLLNDVFLRFFDDICSDGNFLSFTKKNN
                     YWLDDFAIYRVLKRLNNDLPWYHWEKTFADRSEEKISYIERLYEKQVLFEKWVQYICF
                     EQLVNAKQYIGSKGILLMGDMPVLASKDSADVWAKRTLFNLDFIAGAPPDMYCVYGQR
                     WGMPVQNWDNVRKTDFEYIAEKMKWLDNFFDIIRIDHVVGFFRIWAIPVDEPEENQGT
                     NGIFIPQDESLWEDHGKEILSKMNQFSKCLLCGEDLGIIPQVCTETLKEYGIPGYDVQ
                     RWKKNYGIDYSFIPFSEYREIAISTLSTHDTSF"
     gene            complement(1262..>1466)
                     /locus_tag="PLW07_07655"
     CDS             complement(1262..>1466)
                     /locus_tag="PLW07_07655"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the formation of L-histidinol phosphate
                     from imidazole-acetol phosphate and glutamate in histidine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOL50445.1"
                     /translation="FPSSANFLCVKLPDGQAEKIYQYLKEHKILVRYFNTPDLKDYLR
                     ITVGSRSEIYALLRVLKQYYGYR"
BASE COUNT          479 a          294 c          227 g          466 t
ORIGIN      
        1 gaaacttgtg tcatgggttg aaagtgtgga tatcgcaatt tctctgtatt ctgaaaaagg
       61 gataaaagaa taatctattc catagttttt cttccatctc tgcacatcat aaccaggtat
      121 tccatattct ttcagtgttt ctgtacacac ctgtggaata atcccaaggt cttccccgca
      181 caaaagacat ttgctgaatt gattcatttt gcttaatatt tcttttccat gatcttccca
      241 cagcgattca tcctgaggga taaaaattcc atttgtgccc tgattttcct ctggttcatc
      301 aacaggaatc gcccatatcc tgaaaaatcc aacaacatgg tcaatcctta tgatatcaaa
      361 aaaattgtca agccatttca ttttttctgc aatatactca aaatctgttt ttcttacatt
      421 atcccagttt tgaactggca taccccatcg ctgcccatac acacaataca tatcaggtgg
      481 agcgccagca atgaaatcaa gattaaacag tgttctttta gcccagacat ctgcactatc
      541 ctttgaagca agaacaggca tatctcccat aagcaatatg cctttacttc caatatattg
      601 ctttgcatta acaagttgct caaaacatat gtattgaacc catttttcaa aaagcacctg
      661 tttttcatat aaacgttcta tgtatgaaat tttttcttca cttctatctg caaaagtttt
      721 ttcccagtga taccatggca aatcattatt aagtcttttt aagacacgat agatagcaaa
      781 atcatccagc cagtaattat ttttttttgt aaaagataaa aaatttccat cagaacatat
      841 atcgtcaaaa aatctcaaaa atacatcatt cagtaagttg agtttgatat cgcgcgcagt
      901 ataattacat ctgtgatctt ttccgggcaa aaaattcaca tgtaagtcac caatcaaatg
      961 acgagaaaaa ccaaaattgt tatacgaaaa atcttcaagt gaaatataaa cagggtcaat
     1021 agcaaaagaa ctgatggcat tataaggaca gttgtctgcg cccgggtaat taaggggtaa
     1081 aatctgtaaa atagtatcac cggatttttt gcacaaatca gccatgcatt ttaatgtatg
     1141 aaagtcacct gtgcccacgc tggatgatga atgaatggaa aaaagtggcg taagaatccc
     1201 tgcgcgtctg agtgtgccaa ttttttccca ttttttgcca gaagaagttg aaagtaaaaa
     1261 ttcacctgta cccataatat tgtttaagta cccttaaaag agcgtatatc tcactcctgc
     1321 tgccaacagt aatccttaaa tagtccttca aatcaggtgt attaaagtac ctgacaagta
     1381 ttttatgttc tttcaagtat tgataaattt tttctgcctg cccatcagga agtttaacac
     1441 atagaaagtt cgctgatgat ggaaaa
//
LOCUS       DAORYU010000319         1461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_59679_length_1461_cov_2.458037, whole genome shotgun sequence.
ACCESSION   DAORYU010000319 DAORYU010000000
VERSION     DAORYU010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59679_length_1461_cov_2.458037"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /locus_tag="PLW07_07660"
     CDS             <1..959
                     /locus_tag="PLW07_07660"
                     /inference="COORDINATES: protein motif:HMM:NF019736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50446.1"
                     /translation="IEASKHVNLISVSVGANNIKNLMPLLAKIIEKKFVADPSDFLNI
                     IIGENMKNASGIVKQWILEQLSNEAKDFFNRNVGLVETVLSRMVPVVPEELKKQYPLI
                     VLVEPYKTMPVAKNMFKGHIPEIKNFLFVEDIEPYEAMKLYIHNFTHASFACAGHLKH
                     YQFIWESVSDLKIKQAVDRAYLEIQQAINKKYGVSIEELDNYYRDLIERFLNKSLGDT
                     ISRVAREPLRKLGPHDRFIGAAKMCVEQNVIPEYVCFFTACCLYYDQPSDKESQQLQN
                     LLNTKGIDYVLQEVSGLSCSDSIFNMIKDRYVNFCELREKMI"
     gene            990..>1461
                     /locus_tag="PLW07_07665"
     CDS             990..>1461
                     /locus_tag="PLW07_07665"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOL50447.1"
                     /translation="MPKAAVLKSIENLEILEREIPAPQKGEILIRVKSCAICGTDIKV
                     FHHGHKHIVFPRVTGHELSGIIEKTGSDVRNFGEGERVAVAPAIPCGECYYCRRGWQS
                     MCDNLKAIGYHFDGGFAQYMIVPEVAVRNGCVNKIPHNVNFDQAAIAEPLACVIN"
BASE COUNT          490 a          241 c          313 g          417 t
ORIGIN      
        1 ttattgaggc atcaaaacat gtcaatctca tcagtgtttc tgttggcgca aacaatataa
       61 aaaatctcat gccccttctt gcaaaaatca tagaaaaaaa gtttgtggca gatccatcgg
      121 attttctcaa tatcattatc ggggaaaata tgaaaaatgc atccggtatt gtaaaacaat
      181 ggatactgga gcaactttca aatgaagcga aggatttttt taacagaaat gttggattgg
      241 tggaaaccgt tctcagcaga atggttcctg tggtgccaga agaattgaaa aaacaatatc
      301 cattgattgt acttgttgaa ccgtataaaa caatgcctgt tgccaaaaat atgtttaagg
      361 gacacattcc tgaaataaaa aattttctct ttgttgagga tatagaacca tatgaagcaa
      421 tgaaactgta tatacacaac tttactcatg cctcatttgc atgcgcagga cacctgaaac
      481 attatcaatt catctgggaa tcagtcagcg acctaaaaat taagcaggcg gttgacagag
      541 cgtatcttga aattcagcag gcgataaata aaaaatacgg tgtttctatt gaagaacttg
      601 acaattatta cagagacctg attgaaagat tcttgaataa gtcgcttggc gacacaatat
      661 cgagggttgc aagagaacct ttgagaaaac tcggtccgca tgataggttt attggtgctg
      721 caaaaatgtg cgtggaacaa aatgtcattc ctgaatatgt atgctttttc accgcgtgtt
      781 gcctatatta tgaccaacca tctgataaag agagccagca attgcagaat ctccttaaca
      841 caaagggcat tgattatgtt ctccaggaag tatcaggatt gtcctgttct gattcaatct
      901 ttaacatgat aaaagacaga tatgtaaact tttgtgaatt acgggaaaaa atgatataat
      961 tcttttgatt ttatgtacag gtgttaaaaa tgccaaaggc agcagtgttg aaaagtattg
     1021 aaaatcttga aattcttgaa cgagaaatac ctgcgcctca aaagggcgag attttaataa
     1081 gggtgaaaag ttgcgcaatt tgtggaacag acattaaggt atttcaccat ggacacaaac
     1141 atattgtatt tccgagagtc acaggccatg aattaagcgg aattattgaa aaaaccggga
     1201 gtgatgtacg gaattttgga gaaggggaaa gggttgctgt cgcacctgca attccatgcg
     1261 gagagtgcta ctactgtagg aggggatggc aatcaatgtg cgacaatctt aaagccatcg
     1321 gttatcattt tgatggtgga tttgcacaat atatgattgt ccctgaagtg gcagttagaa
     1381 atggttgtgt aaataaaatt cctcataatg tcaattttga ccaggcagcg attgcagaac
     1441 cgcttgcatg cgttatcaat g
//
LOCUS       DAORYU010000320         1460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_59705_length_1460_cov_7.268327, whole genome shotgun sequence.
ACCESSION   DAORYU010000320 DAORYU010000000
VERSION     DAORYU010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59705_length_1460_cov_7.268327"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="PLW07_07670"
     CDS             complement(<1..431)
                     /locus_tag="PLW07_07670"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOL50448.1"
                     /translation="MDFIDYGHSYIQTSGPQNAVRFLIESQMELIDLKTGQTKEFFQV
                     ASCKSENTFARENLFKKDNYNFLPVFLDSEKIIIFRSFEEPSLCMTKTGKILTVMRDG
                     KSGFLWSNYSLDEGKTWSVPVKLDIWGCPANLLSLRDGRIVV"
     gene            complement(434..>1460)
                     /locus_tag="PLW07_07675"
     CDS             complement(434..>1460)
                     /locus_tag="PLW07_07675"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOL50449.1"
                     /translation="GLPDEKFLEMLDPSFKNLRNKLYQKAYPAGVKAGGLSKYWANKL
                     GLCEGTPVSVGAFDAHMGAVGAGIRENVLVKIIGTSTCDIMIFPKDRNLPDIPGLCGI
                     VPDSVVPDFIGLEAGQSAVGDIFYWFLKTFLPEKRKDIYNYYTRLARNIKPGSSGLIA
                     LDWHNGNRTILVDQKLTGLIAGFTLNTKPEEVYRALIEATGFGARVIIDRLDEYGVKA
                     DSIVACGGIAEKNSLLMQIYADITGKQINIAASKQTCALGAAIFGAIAAGKEKSGFSD
                     VRQVQETLCKFKPISYIPDKKNHEIYNLIYQEYKKLHDAFGLESYQGNLYSVMKNLLK
                     IKLRTVG"
BASE COUNT          407 a          313 c          267 g          473 t
ORIGIN      
        1 acaacgattc ttccatccct gagagacaaa agatttgcag ggcatcccca tatatcaagt
       61 ttcactggca cagaccatgt tttgccttca tcaagagaat aatttgacca gagaaaaccg
      121 cttttgccat ccctcattac agtgagaatt tttcctgttt ttgtcataca taaagatggt
      181 tcctcaaatg acctgaaaat aatgattttt tcactgtcca gaaaaacagg aaggaaattg
      241 taattgtctt ttttgaaaag gttttctctg gcaaatgtat tttcgctttt gcatgaagca
      301 acttgaaaaa attcctttgt ctggcctgtt ttcaggtcga taagttccat ctgcgattca
      361 atgagaaatc tcactgcatt ttgaggacca ctggtttgaa tgtaagagtg tccgtaatcg
      421 ataaaatcca tggttaacct actgtgcgca atttgatttt cagtagattt ttcatcacac
      481 tgtagagatt gccctgatat gattccagac caaatgcgtc gtgcaacttt ttgtattcct
      541 ggtatattaa attataaatt tcatgatttt ttttatctgg aatatatgaa attggtttga
      601 acttgcataa tgtttcctgc acctgcctca cgtccgaaaa tccagatttt tcttttcctg
      661 ccgcaattgc gccaaatatc gctgctccaa gagcgcatgt ttgttttgat gctgcaatgt
      721 tgatttgttt tcctgtaatg tcagcataaa tctgcatcag cagggaattc ttttcagcaa
      781 tgccgccaca ggcaacgatt gaatccgcct ttactccgta ctcatcaagt ctatcaataa
      841 tcactcttgc gccaaacccg gttgcttcaa taagagccct gtacacttct tctggctttg
      901 tattgagagt aaaacctgca ataagacctg taagcttctg gtccacaaga attgtcctgt
      961 ttccattatg ccagtcaaga gcaatgagtc cagaactacc tggtttgata tttcttgcaa
     1021 gacgcgtgta ataattgtag atatcttttc ttttttcagg taaaaatgtt ttcaaaaacc
     1081 agtaaaaaat gtctcctact gcagattgac ctgcttcaag tccaataaag tccggaacaa
     1141 cagagtcagg gacaattccg catagtccag ggatatcagg aaggtttctg tcctttggaa
     1201 aaatcataat atcacaggtt gatgtaccaa tgattttgac aagaacattt tccctgattc
     1261 ctgcgcccac tgctcccatg tgagcgtcaa atgcgccaac actcacaggc gttccttcac
     1321 aaagaccaag tttgtttgcc cagtatttcg aaagaccgcc tgcttttact cctgcagggt
     1381 atgccttctg gtaaagtttg tttctcaaat ttttgaatga agggtcaagc atttcaagga
     1441 atttttcatc aggaagtcca
//
LOCUS       DAORYU010000321         1460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_59715_length_1460_cov_3.343772, whole genome shotgun sequence.
ACCESSION   DAORYU010000321 DAORYU010000000
VERSION     DAORYU010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59715_length_1460_cov_3.343772"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..870)
                     /locus_tag="PLW07_07680"
     CDS             complement(7..870)
                     /locus_tag="PLW07_07680"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOL50450.1"
                     /translation="MIIDAHNHPEWHGHTIEKFIENMDKNQIDITWLLTWEAPGDEYS
                     HQHLASIPHFEKYGPIPFSKVLEYVKQRPDRFIPGYAPDPRRPEAIDLLQGAINLYGV
                     RIYGELKIRMMYDNPDAIRMFRFCGKNKLPVVVHIDYEFETGSMYPRPSWWYGGGIDA
                     FERAIIKCPETIFLGHGPGFWAHISGDDKFDKEVYPSGNVIEGGKIIKMLRKHPNLYC
                     DLSAGSGLNALKRDIDFGKNFIIEFQDRLLFARDYFDSKHREFFESLNLPQEVLEKIY
                     CKNALKLVPLK"
     gene            1102..>1460
                     /locus_tag="PLW07_07685"
     CDS             1102..>1460
                     /locus_tag="PLW07_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008679133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOL50451.1"
                     /translation="MKKKGFTLIELLVVIAIIAILAAMILPALSRARKQAKFTRWQAY
                     RTNLVTDETLILYWTFEDGQGVKITNRALGDPRDDEYNRTAYDALFDNTPFPTPANPN
                     PTPTDPTACPTWVKDGGR"
BASE COUNT          430 a          303 c          280 g          447 t
ORIGIN      
        1 atcctgttac ttcaaaggaa caagtttcaa ggcatttttg caataaatct tttccaggac
       61 ttcctgtggt aaattcaatg attcaaaaaa ttccctgtgt tttgaatcaa aatagtccct
      121 ggcaaaaagc aatctgtcct gaaattcaat gataaagttt ttcccgaaat caatatccct
      181 ttttaatgca ttcaatccag aacccgcaga taaatcgcag taaagatttg gatgttttcg
      241 tagcatttta ataattttcc cgccttctat gacattacct gatggataca cttctttatc
      301 aaacttatca tcccctgaaa tgtgcgccca gaatccaggc ccatgaccta aaaatattgt
      361 ttcagggcat tttattattg ccctttcaaa ggcatcaatt ccaccgccat accaccatga
      421 tggtcgtgga tacattgaac ctgtttcgaa ttcgtaatca atgtggacaa caactggcag
      481 tttatttttc ccacaaaatc tgaacattct tattgcgtcc gggttatcat acatcatcct
      541 tattttcagt tctccataga ttcgtactcc gtacaggtta atggcgccct gcaataggtc
      601 aattgcttct ggtcttctcg ggtctggtgc atatcctgga ataaatctat ccggtctctg
      661 ttttacatac tcgagaactt tgctgaatgg aataggtcca tatttttcaa agtggggtat
      721 tgatgcaaga tgttggtggg aatattcatc accaggtgct tcccacgtta aaagccaggt
      781 gatgtcaatt tgatttttgt ccatgttttc aatgaatttt tcaatagtgt ggccatgcca
      841 ttcaggatgg ttatgtgcat caataatcat gtaaattctc cttttggtct tgacaatttg
      901 ttttatgacg gcattatatc actgaaggga caaaatacaa agaggtctgg atgattaaaa
      961 aagacaaggc aacatccaga ggaattttaa aattcccatc caacgatgta cgtaattttg
     1021 cacctggaat ccgatatttc ttttttctgc accagacaaa tttcatccca atcaagttta
     1081 attacaagga ggtgagaatt gatgaagaaa aaaggattta ccttaattga gttgcttgtg
     1141 gtgattgcta tcatagcgat actggcagcg atgattctgc cagcactgtc aagggcaaga
     1201 aaacaggcaa agttcacacg atggcaggca tacaggacaa accttgtgac agatgaaact
     1261 ttaatactgt actggacatt tgaagatggt cagggagtga agattaccaa cagggctctt
     1321 ggagacccga gggacgacga atacaatagg actgcctatg atgctctttt tgataatact
     1381 ccgttcccaa cgcctgcaaa tccaaatccc actccaacag acccaactgc ctgtcctaca
     1441 tgggtaaagg atggtggtcg
//
LOCUS       DAORYU010000322         1451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_60248_length_1451_cov_6.440544, whole genome shotgun sequence.
ACCESSION   DAORYU010000322 DAORYU010000000
VERSION     DAORYU010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60248_length_1451_cov_6.440544"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..140)
                     /locus_tag="PLW07_07690"
     CDS             complement(<1..140)
                     /locus_tag="PLW07_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOL50452.1"
                     /translation="MKSAREKGFEGEQLAAKFLKANGYRILERNYRTKSGEIDIIARK
                     NR"
     gene            complement(143..475)
                     /gene="rplS"
                     /locus_tag="PLW07_07695"
     CDS             complement(143..475)
                     /gene="rplS"
                     /locus_tag="PLW07_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOL50453.1"
                     /translation="MHPKIAALESEIKPKKEIPEFFPGDELEVHIRVQEGEKVRTQVF
                     TGICIARKGTGMRETFTVRKISYGEGVERIFPIYSPNVVSIKLVKSRKDNRLAPRAKM
                     YYLRKKNR"
     gene            complement(450..1166)
                     /gene="trmD"
                     /locus_tag="PLW07_07700"
     CDS             complement(450..1166)
                     /gene="trmD"
                     /locus_tag="PLW07_07700"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014195436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009019 - tRNA
                     (guanine-N1-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOL50454.1"
                     /translation="MRIDIITIFPEIFSPLEASIIKRAQDKCILEICIWNLRDFSRNK
                     HKKVDDAVYGGGKGMVLACEPIFLAVEEVKKHNPESLTILTCPQGEIFSQQKAKELSR
                     EKGLIIICGHYEGVDERVRTIVDCEISIGDYVLTGGELPAMVIVDCVVRLLPGVLPEE
                     APVYDSFFDCLLDWPCYTRPEEFRGLKVPDVLLSGDHKKIAQWRLNQAIERTKQKRPD
                     LHEKFIKEKGGKHASKNSGS"
     gene            complement(1156..>1451)
                     /gene="rpsP"
                     /locus_tag="PLW07_07705"
     CDS             complement(1156..>1451)
                     /gene="rpsP"
                     /locus_tag="PLW07_07705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOL50455.1"
                     /translation="QEKLFIYKEKKMATRIRLVRKGKRNRPCYSVIVISDEKDGRGDV
                     IEIIGHYDPLREPPVFEVKDDRVIHWLKLGAQPSDTVKSFIKKRGLLNQLNAD"
BASE COUNT          372 a          355 c          222 g          502 t
ORIGIN      
        1 cttctgtttt ttctcgcgat gatgtctatt tcgcctgatt ttgttctgta atttctttct
       61 aaaattctgt atccgtttgc tttaagaaat tttgctgcaa gttgttcgcc ctcaaaccct
      121 ttttccctgg cgcttttcat tattatctgt ttttctttct tagataatac atctttgctc
      181 ttggcgcaag acgattatcc tttcttgatt tcacaagttt aatactgaca acatttggtg
      241 aataaatggg aaatattctt tccacgcctt ccccataaga aattttacga accgtgaagg
      301 tctccctcat tcctgtaccc tttcttgcaa tacagattcc tgtaaaaacc tgggtacgaa
      361 ccttttcgcc ctcctgaacc ctgatgtgca cttcaagttc atctcctgga aaaaattcag
      421 gaatttcctt tttcggtttt atctcagatt caagagccgc tatttttgga tgcatgtttt
      481 cccccttttt cttttatgaa tttctcatgc agatcaggcc ttttttgttt tgttctttca
      541 atcgcctgat ttaaacgcca ctgcgcaatt tttttatggt cccctgacag caaaacatca
      601 ggaactttca atcccctgaa ttcctcaggt cttgtatagc acggccagtc aagaagacaa
      661 tcaaaaaatg aatcataaac cggtgcttct tctggcaata ctcctggcaa taatcttaca
      721 acacagtcaa caatcaccat cgctggcaat tctccacctg taagcacata atctccaata
      781 gaaatctcac agtcaacgat ggtacgaacc ctttcgtcca cgccctcgta atgtccgcaa
      841 ataataatca ggcctttttc ccttgaaagt tctttcgcct tctgctggct gaaaatttcg
      901 ccctgcggac atgttaaaat tgtcaaagat tccgggttat gctttttcac ttcttcaact
      961 gcaagaaaaa ttggctcaca tgcaagtacc atccccttgc cgccaccata tacagcatca
     1021 tcaacctttt tatgtttatt gcgtgaaaaa tccctgagat tccagatgca aatttcaagt
     1081 atgcatttat cctgagccct ttttataata cttgcttcca gggggctgaa aatttcagga
     1141 aaaatcgtaa tgatgtcaat ccgcattcaa ttgattaaga aggccccgtt ttttgatgaa
     1201 actttttact gtatcagatg gttgcgctcc aagtttgagc cagtgaataa ccctatcatc
     1261 cttcacctca aacacaggag gttctctgag agggtcataa tgaccaataa tctctatgac
     1321 atctccccta ccatcctttt catcgctgat aacaatcaca ctataacagg gtctatttct
     1381 ttttcctttt ctgacaagac gaattctggt tgccattttt ttctccttat atatgaacaa
     1441 tttctcctgt t
//
LOCUS       DAORYU010000323         1451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_60253_length_1451_cov_4.296562, whole genome shotgun sequence.
ACCESSION   DAORYU010000323 DAORYU010000000
VERSION     DAORYU010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60253_length_1451_cov_4.296562"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="PLW07_07710"
     CDS             <1..852
                     /locus_tag="PLW07_07710"
                     /inference="COORDINATES: protein motif:HMM:NF020892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOL50456.1"
                     /translation="PTPEKYTVYVWFDALLNYLTGVRYKRDEANQYWPPDVQLIGKDI
                     LRFHAIIWPALLMALGLELPEKIFAHGWWTVGSEKISKSRGNIIDPIDLINRFGVDGF
                     RYFLLKEVPFGLDGEFSEEKFIKRFNSDLANDLGNLVNRTLNLVEKLFNGSIPVGYVS
                     SGMEQLVDRVMTDVDKSMNNVSFSDALNEIWKIVGELNKVLDTEKPWNSTLESAKNTL
                     SQCIAGIHLISFFIYPFIPETAQKIWKILNIEHKENNIILNKDVLKIPEGTKSGQREI
                     LFKRIKI"
     gene            849..>1451
                     /gene="pyrH"
                     /locus_tag="PLW07_07715"
     CDS             849..>1451
                     /gene="pyrH"
                     /locus_tag="PLW07_07715"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOL50457.1"
                     /translation="MKPVFKRIMLKLSGEALMGDEKFGIHSKSLISIAEEIKEVTELG
                     CQTAIVIGGGNIFRGITRDANVIDEISGDYMGMLATVINGLALQSCLEKMNAITRVQS
                     AFEMRQFCEPYIRRRAIRHLEKGRIVIFVGGTGNPYFTTDTAAALKAIEIGAEVILKA
                     TKVDGVYTDDPLKNENAKRYSLLTHKDVLAKELKVMDNTAI"
BASE COUNT          494 a          256 c          307 g          394 t
ORIGIN      
        1 ccgacaccgg aaaaatacac agtgtatgtc tggtttgatg ctctcttaaa ttatcttact
       61 ggtgtcaggt acaaacgtga cgaagcaaat caatactggc cacctgatgt tcaactaata
      121 ggcaaggaca ttcttagatt tcatgcaatc atatggccag cattgttgat ggcgcttgga
      181 ctggaattac cagaaaaaat ttttgctcat ggatggtgga cggttggcag cgaaaaaata
      241 tcaaaatcca gaggcaatat tatagacccg attgacttga ttaatcgttt tggcgttgat
      301 ggcttcaggt attttcttct caaagaggtt ccatttggcc tggatggtga attttcagaa
      361 gaaaaattca tcaaacgatt caacagtgac cttgcaaatg atcttggcaa ccttgtcaac
      421 agaacattga atcttgtgga aaaattgttc aatggaagta taccggtcgg ctacgtcagt
      481 tctggaatgg aacaactcgt cgacagagta atgaccgatg ttgacaaatc catgaataat
      541 gtatcttttt cggatgctct caatgaaatc tggaaaatcg tgggagaact caataaagtg
      601 cttgatacag aaaaaccatg gaactcaacg cttgaatctg caaaaaacac actttcgcag
      661 tgtattgcag gtatacacct gatttcgttc ttcatctatc cttttattcc tgaaactgca
      721 caaaaaatat ggaaaatttt gaacatagaa cacaaggaaa ataacattat acttaacaag
      781 gatgttctga aaattccaga aggcacgaaa tccgggcagc gtgaaatcct gttcaaaagg
      841 ataaaaatat gaaacctgta ttcaaaagaa ttatgttgaa actttcaggt gaagcactga
      901 tgggcgatga aaaattcggt attcattcaa aatctttgat atccattgca gaggaaatca
      961 aggaagtgac agaacttggc tgtcaaacag caatagttat tggtggcggc aatattttta
     1021 gaggtattac ccgggatgca aatgttattg atgaaatctc tggcgactac atgggaatgc
     1081 ttgcaactgt catcaacgga cttgctcttc aaagctgtct tgaaaaaatg aacgcgatta
     1141 ccagggttca aagtgcattc gaaatgagac aattctgcga accttacata agaagaagag
     1201 caatcagaca ccttgaaaaa ggcagaattg tgatatttgt tggtggaaca gggaaccctt
     1261 atttcaccac tgatactgcg gctgctttaa aagcaattga aataggcgct gaagtaatat
     1321 tgaaagcaac caaagtcgac ggggtttaca ctgacgatcc attgaaaaat gaaaatgcga
     1381 aaagatacag tttattaacc cataaagatg tacttgcaaa ggaattgaag gtcatggata
     1441 atactgccat t
//
LOCUS       DAORYU010000324         1450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_60308_length_1450_cov_5.759140, whole genome shotgun sequence.
ACCESSION   DAORYU010000324 DAORYU010000000
VERSION     DAORYU010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60308_length_1450_cov_5.759140"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="PLW07_07720"
     CDS             <1..316
                     /locus_tag="PLW07_07720"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006103429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HOL50458.1"
                     /translation="IGVHSIDYSGYPDCRPEFIAAFQNVVNTGVKKSVEGGKIVIRTP
                     LISLKKSEIIKIGNKLGLDFSITWSCYRGTEKACGECDSCKIRLAAFADAGLKDPVQY
                     EN"
     gene            338..1150
                     /gene="lpxI"
                     /locus_tag="PLW07_07725"
     CDS             338..1150
                     /gene="lpxI"
                     /locus_tag="PLW07_07725"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF017989.2"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HOL50459.1"
                     /translation="MNERIGLFASKKDFSVVAFNEIRAMGYNPVVISFTEKSFPEYSD
                     EIVLEFGDIESFVRVLKEKNIKKVVFAGKIEASDIFTKRIAMSGAKFLSTTGNLSPDN
                     ILKKLSEFLTERGIEILPLTEVFKRYLVEEKIYTNTKPDSFQWNDILMGWNIAKSVAA
                     LGIGQAVAIKNGMVIAVEGIEGTDRMIARAGEFCRDFVVVKVIKTEQSDRFDLPTIGP
                     ETIETLGHSGGRVIALEAGKTIMLKQEETIQIANEHKIIIVGFRGKEIGDDC"
     gene            1140..>1450
                     /locus_tag="PLW07_07730"
     CDS             1140..>1450
                     /locus_tag="PLW07_07730"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOL50460.1"
                     /translation="MIVKENRDEILSYLEDSSNFSGGNCDSVYIVENEQEVIEIVQTA
                     NAKKIPLTVSGGGTGTVGGRIPVEGSVISVEKLNRIIDINKDRAILDAGVIVNNFLVE
                     A"
BASE COUNT          477 a          204 c          356 g          413 t
ORIGIN      
        1 gattggggtt cacagtatcg attattcggg ttatcctgat tgcagacctg aatttatcgc
       61 agcatttcaa aatgttgtca atactggtgt gaaaaaatcc gttgaaggtg gtaaaattgt
      121 tatacggacg cctctgattt cgctgaaaaa atcagaaatt atcaaaattg gcaacaaact
      181 ggggctggat ttttccatta cctggtcatg ttacaggggt actgaaaaag catgcggaga
      241 atgtgattcc tgcaagatac ggctcgcagc atttgctgat gcgggtttga aagacccggt
      301 tcagtacgaa aattaaacaa ttaacagggg tgtggtggtg aacgagagga taggtctttt
      361 tgcttcaaaa aaggattttt ctgttgtcgc cttcaatgaa atcagggcga tgggatacaa
      421 tccagtagtg attagtttta ctgaaaagag ttttcctgaa tattcagatg aaattgttct
      481 tgaatttgga gacattgaaa gttttgtgag ggtactcaag gaaaagaaca tcaaaaaggt
      541 tgtttttgca gggaaaatag aagcatcaga tatttttaca aaaagaatcg caatgtcagg
      601 cgcaaaattt ttaagtacaa caggtaattt aagtcctgac aatatattga aaaaactttc
      661 tgaatttctg actgaaaggg gtatagaaat tttgccattg acagaagtgt tcaaacgtta
      721 tcttgtggag gaaaaaatct atacgaacac aaaacctgat agttttcaat ggaacgatat
      781 actaatgggc tggaacattg caaaatctgt tgctgctctt ggtatcggtc aggcagttgc
      841 aataaaaaat ggcatggtga ttgccgttga aggaattgaa ggaacagaca ggatgattgc
      901 aagggcaggc gaattttgca gggattttgt cgtggtaaaa gtgataaaaa ctgaacagag
      961 cgacaggttt gatctaccga caataggtcc tgaaacgata gaaacgcttg gtcattctgg
     1021 tggaagagtt attgcacttg aagcaggtaa aaccattatg cttaaacagg aggaaacaat
     1081 tcagattgcc aatgaacaca agattattat cgtgggtttt agaggaaagg agatagggga
     1141 tgattgttaa ggagaatcgt gatgaaattt tgagttacct tgaggattcc tcaaatttct
     1201 caggcggtaa ttgtgacagt gtttatattg ttgaaaatga acaggaagtg attgaaattg
     1261 ttcagacggc aaacgcaaaa aaaattccgt tgacagtttc cggaggtggt acaggtactg
     1321 ttggcggcag gattccagta gaaggttcag taatttctgt agaaaaactc aacagaatta
     1381 ttgatattaa taaagacagg gcaatacttg atgcaggagt tattgtgaat aactttctcg
     1441 ttgaagccga
//
LOCUS       DAORYU010000325         1446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_60572_length_1446_cov_3.730410, whole genome shotgun sequence.
ACCESSION   DAORYU010000325 DAORYU010000000
VERSION     DAORYU010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60572_length_1446_cov_3.730410"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1446
                     /locus_tag="PLW07_07735"
     CDS             <1..>1446
                     /locus_tag="PLW07_07735"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOL50461.1"
                     /translation="GTYITTPFVSEEKVTVCVKTTIENHTGQTVAVELETVILDCKEN
                     EKARAQITKDIHELAEFQQELTIHSPDLWDIEFPNLYIARSVVKINSETVDCYETRFG
                     IRSIEFHPEKGFFLNGKQVKFKGVCLHHDLGLLGAAINRAAIKRQLTLLKEMGCNAIR
                     TSHNPPAPELLELTDEMGFLVIDEAFDEWKIPKRQNGYHIIFDQWAEKDLKAMIRRDR
                     NHPSVIMWSIGNEIPEQKDPVKGPEIARFLHNICKEEDPSRPTTSAFNMPEEAVKNGL
                     AEIVDVPGWNYQPNNYGKLHQLLPGKPMYGSETASCISSRGEYYFPVEEERGTKRETL
                     HVNSFDQCCPTWATIPDVEFRAQDEHPFIMGEFVWTGFDYLGEPNPYFEEWPSRSSYF
                     GIIDIAGIPKDRFYLYQSRWANKKILHLVPHWTFPGYEGKSIFVQCYSSWNTVELFVN
                     GVSQGIREKSPIALIDRYRLVWNNVVYQPGQ"
BASE COUNT          487 a          243 c          323 g          393 t
ORIGIN      
        1 gggaacttac atcacaacac catttgtaag tgaagaaaag gtaactgtgt gcgttaagac
       61 gaccatagaa aatcatactg gtcaaacagt ggctgtggaa cttgaaacag taattctgga
      121 ttgcaaagaa aatgaaaagg caagagccca aattacaaag gatattcacg aactggcaga
      181 atttcaacag gaattaacga ttcattcacc agatttatgg gacattgaat ttcccaatct
      241 ttacatagcg cgttcagttg taaaaattaa tagtgagacc gttgattgtt atgaaacaag
      301 atttggcata cggtcaatag agtttcaccc tgaaaaagga ttttttctca atggaaaaca
      361 ggtaaagttt aaaggagtat gcttacatca tgaccttgga ttattgggtg cagcaataaa
      421 cagggcagca ataaaaaggc aattgacact gttgaaggaa atgggatgta atgctattag
      481 aacaagtcat aatcctcctg cgccagaact acttgaactt acagatgaaa tgggatttct
      541 tgtcattgac gaggcgtttg atgaatggaa aattcccaaa cgacaaaatg gctatcatat
      601 tatttttgac cagtgggcag aaaaagattt aaaggcgatg atacgaagag acagaaatca
      661 tccttcggta attatgtgga gtatcggtaa tgaaattcca gagcagaaag accctgtaaa
      721 aggtccagag attgcaaggt ttctccataa catctgcaag gaagaagacc caagccgacc
      781 aacaacatct gcattcaaca tgcctgagga agcagtgaaa aatggacttg ctgaaattgt
      841 ggatgtgcct ggttggaatt accaaccaaa taattatggg aaacttcatc aattattacc
      901 aggaaaaccg atgtatggaa gtgaaaccgc atcctgtata agcagtaggg gtgaatatta
      961 ttttccagtt gaagaagaaa ggggaactaa gcgggaaaca cttcatgtaa atagttttga
     1021 ccagtgttgt ccaacatggg cgacgattcc tgatgttgaa ttcagggcac aggatgagca
     1081 tccatttatc atgggcgagt ttgtctggac tggttttgac tatcttggag aaccaaatcc
     1141 gtattttgaa gaatggccaa gtagaagttc ttattttggc attattgata ttgcaggaat
     1201 tccgaaagac aggttctatc tttatcagag caggtgggca aacaaaaaaa tccttcatct
     1261 tgttccacac tggacatttc ctggttatga aggaaaatca attttcgtac aatgttattc
     1321 aagttggaat actgttgaac tttttgttaa tggcgtatct cagggaattc gcgaaaaatc
     1381 tcctatcgca ttgatagaca ggtatcgtct tgtctggaac aatgttgttt accagccagg
     1441 gcaatt
//
LOCUS       DAORYU010000326         1438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_61098_length_1438_cov_3.463485, whole genome shotgun sequence.
ACCESSION   DAORYU010000326 DAORYU010000000
VERSION     DAORYU010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61098_length_1438_cov_3.463485"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PLW07_07740"
     CDS             <1..447
                     /locus_tag="PLW07_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50462.1"
                     /translation="FGWIMECTFIGCPDVMKGIKKVSYILGKVEDDIINSTLVPAKVA
                     IGWSSSTDIWDLALKPENSRLCGNCVYPQERQNLYLILRHLQYPVDILSEEDLMEGYL
                     KNYDVFILVADHLKPAAAQALKNWVQQGGTLISVAGGGFLTITINH"
     gene            456..1121
                     /locus_tag="PLW07_07745"
     CDS             456..1121
                     /locus_tag="PLW07_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50463.1"
                     /translation="MEVFGITDAKLQKEVLALRPKLELVHAKPLDSVVFNNINNKVIG
                     FDIYGYRQSFQAGSGRILATYKNGEPACIENQYGKGKAIIMGFLPGPTYFKPAIPVKP
                     YGRGGIDELQGFVPTSFTDEIPAVFRYFLSGVKSPAVCNEPLVETILRKSDTGYVLFL
                     INYTGKNLKNVRVTFSSEQNESVKSVMSVFSTCHFRKISDGTYQIELKNLEKFDCVKI
                     TKN"
BASE COUNT          474 a          219 c          294 g          451 t
ORIGIN      
        1 tttggctgga ttatggaatg tacatttatc ggatgtcctg atgtaatgaa gggaattaaa
       61 aaggtaagtt atatactggg taaggttgaa gatgacatta tcaattcaac attggtacct
      121 gcaaaggttg ctatcggttg gtcttcttcc acagacatat gggaccttgc tttaaaacca
      181 gaaaatagcc gtctgtgtgg aaactgtgtt tatcctcagg aacgccagaa tttatatctg
      241 attttgagac accttcagta tcctgttgac attctctcag aagaagacct tatggaagga
      301 tatttgaaaa attacgatgt ttttatactg gtggctgacc atctgaaacc tgctgctgcc
      361 caggcattaa aaaactgggt acagcagggt ggcactttga tttctgttgc aggtggtggc
      421 tttttgacaa ttacaataaa ccattgaaca ccctgatgga agtgtttgga attactgatg
      481 caaagttaca aaaggaagtg cttgccttaa gaccaaaact tgaacttgtt catgcaaagc
      541 cacttgattc tgttgttttc aataacatta acaacaaagt tattggattt gatatctatg
      601 gatacaggca gagttttcag gcaggctcag gaagaattct tgccacttac aaaaatggag
      661 aaccagcatg cattgagaat caatatggaa aaggaaaggc aatcattatg ggttttttgc
      721 caggacctac ctacttcaaa ccagcaattc ctgttaagcc atatggaagg ggtgggattg
      781 atgaattgca agggtttgtt ccgacttctt ttactgacga aatcccagca gttttcagat
      841 atttcctttc aggggtaaaa agtcctgctg tgtgcaatga accacttgtt gaaacaattt
      901 tgagaaagtc agatactggt tatgtgcttt ttctgataaa ttatactggt aaaaatctta
      961 aaaatgtgag ggtgacattt tcttcagaac aaaatgaatc agtaaaatct gtgatgtctg
     1021 tattttccac atgccatttc aggaaaattt cagatggtac ttaccagatt gaattaaaga
     1081 atcttgaaaa atttgattgt gtgaagatta caaagaatta gtttctattt tttcataacc
     1141 tttgaaccaa ttggttacaa taaaaatatt ccaacgtacg atgatggaag aaggatctaa
     1201 tttttttcag aagtataaac tgatagtatt gattgagtcc aaaaatatac aaacattgtc
     1261 aatctgaaca tatttctatt tttataaaga atggttgcaa tgaaatatca aaaaacatat
     1321 ggagatagac tggtgtcacg tattaatgtt tactccggga tgaacctgtc cagagactca
     1381 gaaataggaa aataaataaa aaaaggtggt atgtgatatt tagtaaagtg caaattgt
//
LOCUS       DAORYU010000327         1411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_62797_length_1411_cov_4.395280, whole genome shotgun sequence.
ACCESSION   DAORYU010000327 DAORYU010000000
VERSION     DAORYU010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62797_length_1411_cov_4.395280"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1411
                     /locus_tag="PLW07_07750"
     CDS             <1..>1411
                     /locus_tag="PLW07_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOL50464.1"
                     /translation="GKGITFYLQNYFLRSFGTRILADIRLRIYEKVQYLSMEFFGRKK
                     TGELTSRILVDISMMDHALSREIPLLVLNIFQAIVFLIIVLLIDWKLTLVSLVIFPVM
                     LLPILNLSKKLRKISRTLQTNWANLGNIIQESIYGQSIIKAYNQEKTVIEKFAKENEA
                     IFRSNLSIIKRTSLVSPFSELLTTIGGAVIISIGVVKVMNNEFSSGFLFMFIAGLVSL
                     IGPAKAIMNSITYIYMASATLPRIYSVFEEKQTVVDSGTIECPDLKSEIRFVNVSFDY
                     GGKPVLKNINLEILRGEKIGIVGPIGVGKSSLICLLLRLYEPEEGLILFDDIDIKKFT
                     LKSLREKIGFVSQEPIIFSDTIRNNICFGSKNVTEEQFQKVVEITRISSFIDKLKDGY
                     ETIVGERGHTLSGGQKQLICLARAILKNPQILLFDEITASLDSQSEKILHDAIENVMQ
                     NRTVVFVAHRLATVKNMDKI"
BASE COUNT          472 a          231 c          268 g          440 t
ORIGIN      
        1 ggaaaaggta tcacatttta tctacaaaac tactttttaa gaagttttgg aacaagaatt
       61 cttgcggata tcagattgcg catctatgaa aaagttcagt atctttccat ggaatttttt
      121 ggaaggaaga aaacaggcga actcacatca agaattcttg ttgatatcag tatgatggat
      181 catgcgcttt cgagagaaat cccacttctt gtgctgaata tatttcaggc aatcgtattt
      241 cttattattg tgcttttgat agactggaaa ttaacacttg tttcacttgt tatttttcct
      301 gtgatgttac ttccgatttt gaatctcagt aaaaaattga gaaaaatttc aagaacgctt
      361 caaacaaact gggcaaattt aggaaacatc attcaggaaa gcatttatgg acaatcaatt
      421 atcaaggcat ataatcagga aaaaactgtc atagaaaaat ttgcaaagga aaatgaagca
      481 atttttcgtt cgaacctctc aatcataaaa aggacatcac ttgtcagtcc attttcagaa
      541 ctcctcacca caataggtgg agccgttatc atttccatag gtgttgtaaa ggtaatgaac
      601 aacgaattct cctcgggttt tctgtttatg tttattgcag gacttgtgtc cttgattggt
      661 ccggctaaag ccattatgaa ttccatcacc tacatctata tggcaagcgc aacacttcca
      721 aggatttaca gtgtttttga agaaaaacaa acggttgttg attctggaac gattgaatgt
      781 cctgatttaa aatctgaaat tagatttgtc aatgtttctt ttgattatgg aggaaaacct
      841 gttttgaaaa acatcaatct tgaaatactt agaggtgaaa agattggcat tgtcggtccc
      901 atcggtgtgg gcaaaagttc cttgatttgc cttcttttaa ggctttatga gccagaagaa
      961 ggcttgatac tgtttgatga cattgatata aaaaaattca cactaaaaag tttgagagaa
     1021 aaaattggtt ttgtctcgca ggaaccaatt atattttctg acacaataag aaataatatc
     1081 tgctttggca gcaaaaacgt aactgaagaa caatttcaaa aagttgtaga aattacaagg
     1141 atttcaagtt ttattgacaa attgaaagac ggctatgaaa caattgttgg agaaagaggc
     1201 catacccttt cagggggcca gaaacagttg atatgtcttg ccagggctat tctgaaaaac
     1261 ccgcagatac ttctgtttga tgaaattact gcttcacttg acagtcaatc agaaaaaatt
     1321 cttcatgatg cgattgaaaa cgtgatgcaa aatcgcacag ttgtttttgt cgctcacagg
     1381 cttgcgacag taaaaaacat ggataaaatc g
//
LOCUS       DAORYU010000328         1411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_62827_length_1411_cov_2.098820, whole genome shotgun sequence.
ACCESSION   DAORYU010000328 DAORYU010000000
VERSION     DAORYU010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62827_length_1411_cov_2.098820"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="PLW07_07755"
     CDS             <1..495
                     /locus_tag="PLW07_07755"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOL50465.1"
                     /translation="LNQGAIDVFIADSQQKQDDILYVRSMVYEALKFETIEILDVSLP
                     PDQMATHISSVDNISAKYNVWLPTYGHAGDGNLHTHIMKITPGGDKITDWKNIFMEIR
                     NEIIFDGIQKGGKISGEHGIGLIKKQYLEMCLGKTSIEIMKKIKQAFDPLYILNPGKI
                     FDPD"
     gene            complement(498..>1411)
                     /locus_tag="PLW07_07760"
     CDS             complement(498..>1411)
                     /locus_tag="PLW07_07760"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOL50466.1"
                     /translation="GFQTVFDGIQGEIKAVELSEKLDGNLFAFLSWFDRTKGSKLYNP
                     SSDTILPARIILVDSYDMGKTWENYRTIDIGDLQGPALTGPVVKVPDGYLVFFEKYGP
                     EQPSGPSLHAACALFSKDGIIFDRIITVARHPENKIFYWDQRNAYDVDSGKIISMFWT
                     YDRENEKDIDIHIAYGNPDTLTWTVPQSTGIQGQIAMPIPLTEGRLLCFYVHRHHPGS
                     MRLVMSYDKGKTWDIANEMVIYENQEIKEKGVSGESSYAEYWEDMGTWSFGHPCGIML
                     DENLGLLVYYAGRDVKKLSARYAIVSI"
BASE COUNT          439 a          284 c          241 g          447 t
ORIGIN      
        1 ctcaatcagg gtgcgattga tgtttttatt gcagattccc agcaaaaaca agatgatatc
       61 ctttatgtaa gaagcatggt atatgaagca ttaaaatttg agacaatcga gattcttgat
      121 gtttcgctac ctccagatca gatggcaacg cacatttcgt ctgttgacaa tatttctgca
      181 aagtataatg tttggttacc tacctatgga catgcaggag atggcaatct tcatactcat
      241 ataatgaaga ttactcctgg gggagataag attaccgact ggaaaaatat ttttatggaa
      301 ataaggaacg agataatctt tgatgggata caaaagggcg ggaaaatttc tggagagcat
      361 ggaattggac tgataaaaaa acaatatctt gagatgtgcc ttggtaaaac ctccattgaa
      421 attatgaaaa aaatcaaaca ggcatttgat ccactttaca ttctaaatcc tggcaagatt
      481 tttgacccgg actgagttta tatagaaaca attgcgtatc tggcacttaa ttttttcacg
      541 tctcttcctg cataataaac aagaagtcca agattttcat caagcataat cccacaggga
      601 tgtccaaaac tccatgttcc catatcttcc cagtattcag cataggaact ttctcctgat
      661 accccttttt cttttatttc ctgattctca taaataacca tctcatttgc aatatcccag
      721 gttttaccct tatcataaga catcaccagc cgcatagaac caggatgatg cctgtgaaca
      781 taaaaacata aaaggcgtcc ttcagtcagt ggaataggca ttgcaatctg accctgtatt
      841 cctgttgact gtggaactgt ccaggtaagt gtatcaggat ttccataagc aatatgaata
      901 tctatatcct tttcgttttc tctgtcatat gtccagaaca ttgatataat ctttcctgaa
      961 tcaacatcat aagcatttct ctgatcccag taaaaaatct tgttttcagg atgtctggca
     1021 acagtaataa tcctatcaaa gattattcca tctttactga aaagagcgca ggcagcgtgt
     1081 aaagaaggac cagatggctg ttctggccca tatttttcaa aaaataccaa ataaccatct
     1141 ggaactttta ccactggtcc tgttagtgct ggcccttgca ggtctccaat atcaatagtc
     1201 ctataatttt cccatgtctt tcccatatca tatgaatcaa caagaattat ccttgctggt
     1261 agtattgtat cacttgatgg gttatatagt tttgaccctt ttgttctgtc aaaccatgac
     1321 aggaaagcaa aaagattacc atctagtttt tcagatagtt caactgcttt tatttctccc
     1381 tgtattccat caaatactgt ttgaaatcca g
//
LOCUS       DAORYU010000329         1410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_62851_length_1410_cov_6.470849, whole genome shotgun sequence.
ACCESSION   DAORYU010000329 DAORYU010000000
VERSION     DAORYU010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62851_length_1410_cov_6.470849"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="PLW07_07765"
     CDS             complement(<1..656)
                     /locus_tag="PLW07_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HOL50467.1"
                     /translation="MLVSIGSIIIAFLFALEILRKDAGNEKMQEIANAIKEGAGAFLK
                     RQYTTIAVLSITLAIIIFLIYALSGKLTYGWHTSFAFILGALCSSISGYVGMYISVRS
                     NSRAASAAGRSMNEALVTALKGGAVSGILVVALSLLGVAGIFLLYGGSANPLEVPLLI
                     VGFGFGASFVALFAQLGGGIYTKAADVGADLVGKVEAGIPEDDPRNPAVIADLVGDNV
                     G"
     gene            878..>1410
                     /locus_tag="PLW07_07770"
     CDS             878..>1410
                     /locus_tag="PLW07_07770"
                     /inference="COORDINATES: protein motif:HMM:NF012893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HOL50468.1"
                     /translation="MARLQETGITELVGLTQEQVFSRFGTSIKGLSDEEAEQRLQEYG
                     FNELFKPKKRSIVFQIASKFLNPLVIVLLIIAIFSIVFGEIASAILVILMAIGSVSLS
                     FFQEHRAGKEAEKLSEMVRPTATVYRNGITKEIPIREIVPGDIVYLSAGDMVPADLRI
                     VSCKDLFVNQASLTGES"
BASE COUNT          452 a          271 c          285 g          402 t
ORIGIN      
        1 ccaacattgt ctccaacaag gtcagcaatg acagcaggat ttctcgggtc gtcttcaggt
       61 attcctgcct ccacctttcc aaccaggtca gcgccaacat cagcggcttt tgtatagata
      121 ccaccaccga gttgcgcgaa aagtgctaca aatgatgctc cgaatccaaa accgacaatt
      181 aatagcggta cctccagtgg attggcgcta ccgccataaa ggagaaatat tcctgctaca
      241 ccgaggagag acagagccac tacaagaatt cctgaaactg ccccaccttt taaggcggtg
      301 acaagcgctt cattcatact gcgtcctgcg gcagatgcag cgcgagaatt tgacctgaca
      361 gatatgtaca ttcctacata tcctgaaatt gaagaacaaa gagcaccgag aatgaaagca
      421 aaagaggtat gccagccata ggtaagtttg ccactgagtg cgtaaataag gaatataatg
      481 atagccagtg tgattgagag aacagcaatt gttgtatact gtctttttag aaaagcgcct
      541 gcgccctctt ttattgcatt tgcaatttcc tgcatttttt catttccggc atcctttctt
      601 aaaatttcaa gggcaaacag gaaagcaata atgatacttc caatacttac cagcatcacc
      661 agattgaaat acatatttac ctccatgttg aaaaattggc attattttaa caccaaaaaa
      721 aattttttca aacatctgat aaaatttgtt cagttatatg aaaaaaattc aatggcaagc
      781 attaatctta cattgcagaa tgaatagtaa ttttttggta tggtggtaaa aaggttataa
      841 taatttacga ttgaaaaaat tttataagga ttcaacaatg gcgcgattgc aggaaacagg
      901 aattactgaa ctggttggcc tcacccagga acaggttttt tcaaggttcg gtacatcgat
      961 aaagggtctg agtgatgagg aagcagaaca aaggcttcag gagtatggtt ttaatgagct
     1021 ttttaagccc aaaaaacgct caatcgtatt tcaaattgct tcaaaattct tgaatcccct
     1081 ggttattgtt ttactgatta ttgcaatttt ttccattgtt tttggagaaa tagccagtgc
     1141 aatactggta atcttgatgg caataggaag tgtttcttta tcattttttc aggaacaccg
     1201 cgcggggaaa gaagcagaaa aactcagcga aatggtgaga ccaaccgcta cggtttatag
     1261 aaatggtata acaaaagaaa ttccaataag agaaatagtt cctggtgata ttgtatatct
     1321 ttctgcagga gacatggttc ctgctgattt aagaattgtt tcgtgtaagg atttatttgt
     1381 taaccaggcg tcacttacag gagaatcctt
//
LOCUS       DAORYU010000330         1401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_63464_length_1401_cov_5.289747, whole genome shotgun sequence.
ACCESSION   DAORYU010000330 DAORYU010000000
VERSION     DAORYU010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63464_length_1401_cov_5.289747"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..>1401
                     /locus_tag="PLW07_07775"
     CDS             87..>1401
                     /locus_tag="PLW07_07775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOL50469.1"
                     /translation="MCSIISYAIARHRILNISVFAEKTFVYGCLIAGLMLVYTFFVWS
                     LSHFVKIFTTGTSLFPVIASSFIIAIAFSPLKEIIQRWARKKIFRQQYDIEQILAQMR
                     LIVGSSLNFEEGIAAMMGIIDNEIHIKNIPVFIIKHEQSARKIFMELKRNSMYRIEFN
                     DSSSILGLLETEPFTHFKDELIRFSGRETVKSVLTEMDFYGAEVIIPIQFHNRVIGTI
                     LFSGREDEKSFSDTERRILNALSLYMGIFIETTALATTLRENRSYQQSLLENLPSGVI
                     AVDDEHNVIVFNYEAERITGLKRENVLRKKYEEVVPADLQKLLTFLLRNKNGIRHMET
                     EFSKNGSPIPLMASGSCFYSSGGSLLGAQLIFSDISQLKILQDQVKRNERLASLGILA
                     AGIAHEIRNPLVALKTFSQLLHEKFLDEEFRVNYTKVVIPEIERID"
BASE COUNT          451 a          206 c          283 g          461 t
ORIGIN      
        1 caatttttgc agttgttacg acacttattg ctcccatgtt aggaacaacc attttatgca
       61 gatttgggtc aatgtcttcc attattatgt gttccataat atcttatgca atcgcaagac
      121 acagaatttt gaatatttct gtttttgcag aaaaaacatt tgtttatggt tgtttgattg
      181 caggattgat gctggtgtat acattctttg tctggtcttt atcccatttt gtcaagatat
      241 ttaccactgg aacatcattg tttcctgtta ttgcaagttc ctttattatc gcgattgctt
      301 tttcaccctt aaaagaaatt atccagagat gggcaagaaa aaagatattc aggcaacaat
      361 atgatataga acagattctg gctcaaatga gattgattgt aggaagttct ttgaattttg
      421 aagaaggtat tgccgcaatg atgggaataa tcgacaatga aatccatatt aaaaatatac
      481 ctgtttttat cataaagcat gaacagtcag caagaaaaat tttcatggaa ttgaaaagaa
      541 attctatgta caggattgaa tttaatgata gttcttcgat tttagggttg ctggaaacag
      601 aaccttttac tcattttaag gatgaactta tcagattttc aggcagagag acagtaaaat
      661 ccgtattaac agagatggat ttctatggcg cagaagttat cataccaatt caatttcata
      721 acagggtcat tggaacaatt ttattcagtg gcagggaaga cgaaaagagt ttttcagata
      781 ctgaaagaag aatattgaat gcgctgtcgt tgtacatggg aatatttatt gaaaccactg
      841 cccttgcaac taccctcagg gaaaacagga gttatcaaca atcgttgctt gaaaatctac
      901 cgagcggggt gatcgctgtg gatgatgaac acaatgttat tgtttttaat tatgaagccg
      961 aaagaattac agggttgaaa agggaaaatg ttttaagaaa aaaatatgag gaagtggtac
     1021 cggctgactt gcaaaaacta ctgacatttc ttttgcggaa taaaaatgga atcagacata
     1081 tggagacgga attttctaaa aatggttctc caattccatt gatggcgagt ggttcgtgtt
     1141 tttattcatc tggcggttct cttcttggag ctcaattgat tttttctgat atttctcaat
     1201 tgaaaatact tcaagatcag gtgaaaagaa atgaaaggct tgcttctctt ggcatccttg
     1261 ctgcaggtat cgcacatgaa attagaaatc cgcttgtggc tttgaagacg ttttcacagc
     1321 tattgcatga aaaatttttg gatgaagaat ttagagtaaa ttatacaaaa gttgtgattc
     1381 ctgaaattga aagaattgat g
//
LOCUS       DAORYU010000331         1401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_63471_length_1401_cov_4.118871, whole genome shotgun sequence.
ACCESSION   DAORYU010000331 DAORYU010000000
VERSION     DAORYU010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63471_length_1401_cov_4.118871"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..732)
                     /locus_tag="PLW07_07780"
     CDS             complement(103..732)
                     /locus_tag="PLW07_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOL50470.1"
                     /translation="MKHKTGFTLIELLVVIAIIGILAAMVLPALARARENGRRAICTN
                     NLKQLSLALEMYADDNDEFYPICGGTIDWGETPPGWMEQLFPYVKNKKMYQCPSFPRN
                     VSEYHYFLSARAAYIDAGGNIAATNRKRIVFPSAFVLAGDSNYRFAEPDCDKDDYTQN
                     CLGWTADNQHWKPHHAGGLNVLFADGHVGWYTEYDPDSMTFRYSTFSDW"
     gene            complement(805..>1401)
                     /locus_tag="PLW07_07785"
     CDS             complement(805..>1401)
                     /locus_tag="PLW07_07785"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Catalyzes first step of the de novo purine
                     nucleotide biosynthetic pathway; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOL50471.1"
                     /translation="MDSIIEGVPVVTARKNLGASLAKNDHVDADIVAAIPFSGIGYAL
                     GYHKESKIPYDEVFLIDRFASRSYLPLTQEERDREAKIKLSVIEENVRGKRIILCDDS
                     IVRGTQIRNKVLELKAFGAKEVHVRVGCPPLLAPCLYDISTRSYKELAAKRYSINQIT
                     KKIGADTLKYNSVEDFVKALGLPEHHLCLHCWTEKKII"
BASE COUNT          400 a          309 c          259 g          433 t
ORIGIN      
        1 aactttataa agaggggagt agaatggggt tttgtaaggg aaggtgacct gaagtaagag
       61 ggggatttta caaaagaaga cctgaagtga gcagagccct tgttaccagt cagaaaatgt
      121 tgaatacctg aaagtcattg aatcagggtc atattctgtg taccatccca catggccatc
      181 agcaaacaac acattcaatc cacctgcatg atgcggtttc cagtgctggt tatcagcagt
      241 ccagccaagg caattctggg tataatcatc cttgtcacaa tctggctcag caaatctgta
      301 attggaatct cctgcaagca caaaggctga tggaaacaca attcgttttc tgtttgttgc
      361 ggctatattt cctccagcat caatatacgc agcgcgcgca ctcaaaaaat agtggtattc
      421 agaaacattt cttggaaatg aaggacactg atacattttt ttatttttta cataaggaaa
      481 taattgttcc atccatccag gaggggtttc gccccagtca atcgtgcctc cacagatggg
      541 ataaaattca tcattgtcat cagcatacat ctcaagagcc agacttagtt gttttaaatt
      601 atttgtgcat attgcccttc ttccgttttc tcttgccctt gcaagtgcag gtaacaccat
      661 tgcagcaaga atgccaatga tagcaatgac cacaagaagt tcaatcagag taaatccagt
      721 tttatgtttc atctcactcc tttgtaaaca cgtcattatt ttgacttcaa aaatttcagg
      781 tggtttagtc aggtaggcaa cctattaaat aattttcttt tctgtccagc agtgcagaca
      841 cagatgatgt tcgggtaacc ccagtgcctt cacaaaatct tcaactgaat tgtatttcaa
      901 tgtatcagcg ccaatctttt tagtaatctg attgattgaa tatcgttttg ccgcaagttc
      961 cttgtatgag cgggttgaaa tatcataaag acagggtgcc agcagtggcg ggcatcccac
     1021 cctcacatga acctcttttg ctccgaatgc cttcagttcc agtaccttat ttctgatttg
     1081 agtgcctctt acgattgagt catcacacag gataattctt ttccctctca cgttttcttc
     1141 aataaccgat agtttaattt ttgcttccct gtctctctct tcctgtgtca atggaagata
     1201 acttctgctt gcaaatctat ctatcagaaa cacctcatca taaggaattt ttgactcttt
     1261 atgataaccg agagcatatc cgattccaga aaaaggaatt gcagcaacaa tatcagcatc
     1321 cacatgatca ttttttgcta aacttgctcc aagatttttt ctcgctgtga caaccggcac
     1381 accttcaata attgaatcca t
//
LOCUS       DAORYU010000332         1393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_63941_length_1393_cov_4.799701, whole genome shotgun sequence.
ACCESSION   DAORYU010000332 DAORYU010000000
VERSION     DAORYU010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63941_length_1393_cov_4.799701"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="PLW07_07790"
     CDS             complement(<1..373)
                     /locus_tag="PLW07_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50472.1"
                     /translation="MKKFLIPVAVSILVLANFSMARTKLVALPERADTVIRLDNPFYT
                     LVEEERVLALQKGTNQVDFSWTGVSIDPDSIRIKIISHPDEVKLISVSYPPGENACIW
                     QIYSPSPYEEKIRISYLLKNID"
     gene            complement(435..>1393)
                     /locus_tag="PLW07_07795"
     CDS             complement(435..>1393)
                     /locus_tag="PLW07_07795"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOL50473.1"
                     /translation="LIGRNNLGLLKWTQVHQFITPDCVSEKIKFEKNGIRGQRRIIYT
                     PVGSIFEEVLYEKIIGGGSIKKHFIKEKKDYEIFLFYLKNIIVKENLTLFLNDLKLLG
                     DDGLPHVSLGRTSYQQLWIQWVLIDDVCQHLVDFPDLMEEVIQTMNNIHRKIFLIARN
                     VAKRYQVPYVLFPDNITSPMIGEKYFKKYCVPLYNECAEILKDTGTLIGVHMDGDLKA
                     LWDAISQSQVRVIDSLSVPPDNDTDVKTAISIWPETRLLINFPSSVHLKSSDEIYQKA
                     MEILNQGGKTGRIWIQISENMPPDAWKKSYPAIVKAINDFGM"
BASE COUNT          389 a          273 c          264 g          467 t
ORIGIN      
        1 tatcaatgtt tttgagaaga taactgattc ttattttttc ttcgtatggt gaagggctgt
       61 aaatctgcca gatgcacgcg ttttctccag gggggtaact cacgctgatg agttttacct
      121 catcaggatg ggaaatgatt ttgattctga ttgagtcagg gtcaatactg acgcctgtcc
      181 aggaaaaatc aacctggttt gttccttttt gaagcgccag tactctttct tcttcaacaa
      241 gggtataaaa aggattgtca agccgtatga ctgtgtctgc cctttctgga agcgcaacaa
      301 gttttgttcg tgccattgaa aaatttgcca gaaccaggat tgatactgct acaggtatga
      361 gaaatttttt cataactcct ccgtgtttga tgtttgtttt gacctttaga cagaccagaa
      421 caggaaaaag ttccctacat tccaaaatca ttgattgcct tgacgatcgc aggataactt
      481 ttcttccatg cgtctggcgg catgttttct gatatctgta tccagattct gcctgttttt
      541 cctccttgat ttaatatttc catcgccttc tggtaaattt catcagatga tttgaggtga
      601 actgaagaag gaaaattaat caatagacgt gtctcaggcc agattgaaat tgcagttttg
      661 acatcagtat cattgtcagg aggcacagaa agtgagtcaa taacccttac ctgtgattga
      721 gaaatggcat cccacagggc tttcaaatct ccgtccatat gtacgccaat caatgtgcct
      781 gtgtctttca atatttcagc gcattcattg tacaggggca cacagtattt tttgaagtat
      841 ttttctccta tcattggaga tgtgatattg tcgggaaaaa gcacataagg aacctgatat
      901 cgttttgcca catttctcgc aataagaaag attttcctgt gaatgttgtt cattgtttgt
      961 atcacttctt ccattaaatc agggaaatca acaagatgct ggcacacatc atctatcaat
     1021 acccattgta tccataattg ctggtatgag gttctaccaa gagacacatg gggaagtcca
     1081 tcatctccaa gtaatttcag gtcattgaga aacaatgtaa gattttcttt tacgatgatg
     1141 tttttcagat aaaacaaaaa aatctcgtaa tctttcttct ctttaatgaa atgctttttt
     1201 attgagccgc cgccaataat tttttcataa agaacttctt caaaaattga acccactggt
     1261 gtataaatga tacgcctttg gcctctgata ccattttttt caaactttat tttttcgctg
     1321 acacagtcag gcgtaataaa ctggtgaacc tgagtccatt tcaaaagtcc aagattgttt
     1381 cttccaataa ggt
//
LOCUS       DAORYU010000333         1392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_64001_length_1392_cov_4.142857, whole genome shotgun sequence.
ACCESSION   DAORYU010000333 DAORYU010000000
VERSION     DAORYU010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64001_length_1392_cov_4.142857"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /gene="coaE"
                     /locus_tag="PLW07_07800"
     CDS             <1..378
                     /gene="coaE"
                     /locus_tag="PLW07_07800"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOL50474.1"
                     /translation="HLNKIIHPYVFEIMEKKLDTYRKRCKMAVVVEIPLLFETRSEKR
                     FDIIITVFSPCQAIRERLKKRYSPQEIEMRLKSQMPLEKKMRLSDYVIDNSGSLFETR
                     MQVKKILQEIMGRQSIWQKKSKN"
     gene            354..>1392
                     /gene="rho"
                     /locus_tag="PLW07_07805"
     CDS             354..>1392
                     /gene="rho"
                     /locus_tag="PLW07_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOL50475.1"
                     /translation="MAEEIKELNIAILKKTAISRLQKLARDMGVNGVSGLTKEELIYR
                     IMQAQAEKNGLLFGEGVLEILPDGFGFLRSSNHNYLPGPDDIYVSPSQIKKFGLKTGD
                     TVSGQIRPPKENEKYFALLKVEMVNSEEPEKIQDRVPFEELTPIHPDKRFILETKSEE
                     ITTRVIDLICPIGKGQRGLIVSPPRAGKTVILQKIANAIAINNPEVHLIVLLVGERPE
                     EVTEMKRSVKGEVVSATFDEPPDRHVQVADVALERAKRLVEHKKDVVILLDSITRLAR
                     AHNVLVPHSGKIMTGGLESNALDKPKRFFGSARNVEEGGSLTILGTALIDTGSKMDDV
                     IFEEFKGTGNME"
BASE COUNT          467 a          236 c          326 g          363 t
ORIGIN      
        1 catcttaaca aaattataca tccgtatgtt tttgaaatca tggagaaaaa acttgacact
       61 tatcggaaaa ggtgtaaaat ggcagttgta gttgaaatac ctcttctttt tgaaacccga
      121 agcgagaaaa ggtttgatat tattattact gttttttccc catgtcaggc aataagagaa
      181 aggttaaaaa aaaggtattc tccgcaggaa attgaaatgc gtttgaagtc gcagatgccc
      241 ttagagaaga aaatgcgttt gtccgattat gtgattgaca attcaggttc tctttttgaa
      301 accaggatgc aggtgaaaaa gatactgcaa gaaataatgg ggaggcaatc aatatggcag
      361 aagaaatcaa agaattgaat attgcgattt tgaaaaaaac agcaatctca aggttacaaa
      421 aacttgcaag ggatatggga gtcaatggtg tcagtggtct gactaaggaa gaactaattt
      481 acagaatcat gcaggctcag gctgagaaaa atggactttt gttcggagag ggagttcttg
      541 aaattcttcc tgacggattt gggtttcttc gctcttcaaa tcacaactat cttccgggtc
      601 ctgatgacat ttatgtttca ccctcacaga taaaaaaatt cggattgaaa acaggagata
      661 ccgtatcagg gcagatcaga cctccaaaag agaacgaaaa atactttgcg cttctgaagg
      721 ttgaaatggt taattctgag gaaccggaaa aaattcagga tcgcgttcca tttgaagaat
      781 taacgccaat tcatcctgac aagcggttta ttcttgaaac aaaatctgaa gaaattacga
      841 cccgcgttat tgacctcatc tgtcccatag gcaaagggca gaggggatta attgtatctc
      901 ctccgagggc aggaaagacc gtgatacttc agaaaatcgc caatgccatt gccataaaca
      961 atccagaagt acatctgata gtacttcttg ttggcgaaag accagaagaa gttactgaaa
     1021 tgaaaaggtc tgtgaaagga gaagttgtca gcgcaacctt tgatgagcct cctgacaggc
     1081 atgtgcaggt agctgatgtt gcgcttgaaa gggcgaaaag gcttgtggag cacaaaaaag
     1141 acgttgtgat tttacttgac agtatcacac ggcttgccag ggctcacaat gtgctggttc
     1201 ctcatagcgg caagatcatg accggcgggc ttgaatcaaa cgcacttgac aaaccaaaaa
     1261 ggttttttgg ttcagcaaga aatgttgaag aaggaggcag tttaacaatt cttggaacag
     1321 cgcttattga tacaggaagt aaaatggatg atgtgatttt tgaagagttt aagggaacag
     1381 gaaatatgga aa
//
LOCUS       DAORYU010000334         1379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_64899_length_1379_cov_2.814199, whole genome shotgun sequence.
ACCESSION   DAORYU010000334 DAORYU010000000
VERSION     DAORYU010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64899_length_1379_cov_2.814199"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1204)
                     /locus_tag="PLW07_07810"
     CDS             complement(<1..1204)
                     /locus_tag="PLW07_07810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOL50476.1"
                     /translation="MNIFLINSYDIFGGAEKIVYELTHIYHQMNHRVRLVVAEKFSES
                     PLVIEVPDDKTPSINSRIFKKINQRYSKPDIWKKILGHLEQPGGIKRMFTGREKYGNT
                     GMETIINKSGFEPDIVHFHNLRKNPFGPVLLHFTTMKFPIVITLHDLWFVTGKCIQPG
                     NCDEWRNKCRNCPEAWFPFFLTRRGIAKNFREKMHLMSQSKPYICAPSQWIMNYVKNS
                     FISEVAAGLQVIPNGINLSTFTPGDKFAARQKLALPENTSIVLTAGKELRTNKYKNFG
                     TFRKIAMMMGKIYTHKPLLFLCLGDSGHSEFYHNAEIRFIPFENNEIIVADYYRAADV
                     YVSTATFETWGLTISEAMACGTPVVAFSNGGVTEQITDGKTGFLVDSNEPIKMAEIIT
                     TLLENEQLH"
BASE COUNT          374 a          245 c          265 g          495 t
ORIGIN      
        1 tatgtagttg ttcgttttca agcaatgtgg tgattatttc tgccatcttt attggttcat
       61 ttgagtcaac cagaaatcct gttttgccat ctgtaatctg ttctgttact ccgccgtttg
      121 aaaacgcaac gactggggtt ccgcatgcca tggcttctga aattgtgagt ccccatgttt
      181 caaaggttgc agtgctgaca tatacatctg ctgccctgta ataatcagca acaattattt
      241 cgttgttctc aaaaggtatg aatcttattt ctgcattgtg ataaaattct gaatgtcccg
      301 aatctccaag gcagaggaac agtaatggtt tgtgagtata aatttttccc atcatcatcg
      361 caatttttct aaaagttcca aaatttttat atttgtttgt tctcaattct tttccggcag
      421 taagaacaat actggtgttt tcgggtaatg caagtttttg tctcgctgca aacttgtcgc
      481 caggcgtgaa cgttgaaaga ttaatcccat tggggattac ttgcaatcct gcggcaactt
      541 cgctgataaa ggaatttttg acataattca taatccattg tgacggtgcg cagatatagg
      601 gttttgattg agacattaaa tgcattttct ctctgaagtt ttttgctatc cctcgccttg
      661 ttagaaagaa tgggaaccat gcttccggac aatttctgca tttattccgc cattcatcgc
      721 aattccctgg ctgtatgcat ttccctgtta caaaccagag gtcgtgcagt gtaataacaa
      781 ttggaaattt cattgttgtg aagtgaagta gtacgggacc aaaaggattt ttccgtaaat
      841 tgtgaaagtg gacaatatca ggttcaaaac cagatttatt aattatagtt tccatgcctg
      901 tattaccata tttttctcta cctgtgaaca ttctctttat tccaccaggt tgttcaagat
      961 gtcctaaaat ttttttccag atgtctggtt ttgaatatcg ttgattgatt tttttgaaaa
     1021 ttcttgaatt gatggatggt gttttgtcat ctggaacttc aataacgaga ggactttcag
     1081 aaaacttctc tgccacgacc agccgtacac gatggttcat ctgatggtaa atatgagtaa
     1141 gttcatatac aattttttct gcaccaccga atatgtcata agagttgata agaaaaatgt
     1201 tcatatgaat gtagttatac tatttcagtt tttcgcatat ttgttattat aatcattttc
     1261 aatgaaactt gtaaattcaa atttcttttg tttactgaaa agaaattata agttataata
     1321 ttttcagggg gtatagctca atgggagagc gtccgccttg cacgcggaag gttgtcggt
//
LOCUS       DAORYU010000335         1372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_65389_length_1372_cov_5.897494, whole genome shotgun sequence.
ACCESSION   DAORYU010000335 DAORYU010000000
VERSION     DAORYU010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65389_length_1372_cov_5.897494"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="PLW07_07815"
     CDS             <1..203
                     /locus_tag="PLW07_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012940657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50477.1"
                     /translation="AMIRDRRDHPENCGCKGQYFSVCGGCVARAYAYYGDFMAPDPGC
                     IYNSVDKEVVGATDRKYTFQSR"
     gene            complement(207..1286)
                     /locus_tag="PLW07_07820"
     CDS             complement(207..1286)
                     /locus_tag="PLW07_07820"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOL50478.1"
                     /translation="MSTNDFEFKKNFAETLERHKKLQSAKKGCLIYTFLPEEEEIVNS
                     VKVPQLEEIDFKKNVLLLPKLLCDREEKLYRYRSDYPDDSIPVLALRYGSGIFAGMIL
                     GSLRFGANTSWIEPVGKTIEETINFPWKKDNEFIDIALTGLQYATKRMKNKCYVYLSG
                     YHSPLELAFILRGSEFFLDVYECPDKVHTLIKRCDEALKWVYQLIENNVKNENYGNIA
                     GFLWMERCLSFLSEDAAGLLSPQHYKEFGVPYTNEVFKRFGGGFLHVHTQSYHQMENI
                     SSIEYLTTYNWRPDPNTPEAISILDKLLDGAKKKIVVIVATPRAIQEKIEILSQGRFF
                     LLCYCKDKNEQQSIVNFVKKMAPIE"
BASE COUNT          406 a          259 c          254 g          453 t
ORIGIN      
        1 tagcgatgat tagagataga agggaccatc cagaaaattg tggatgtaaa ggacagtatt
       61 tttcagtttg tggcggctgt gtggcaagag catacgcata ctatggagat tttatggcac
      121 ctgacccagg ttgcatctat aattctgtag ataaagaagt tgttggcgct actgatagga
      181 aatacacttt tcaatcaaga taattctcat tcgatggggg ccattttttt aacaaagttc
      241 acgatagatt gttgctcatt tttgtctttg cagtagcaaa gcaagaaaaa tcttccctga
      301 gatagaatct cgattttttc ttgaatggcc cggggggttg caacaattac aacaattttc
      361 ttttttgcac catcaagcaa cttgtcaaga atactgatgg cttcaggtgt atttggatca
      421 ggtctccagt tgtaggtcgt aagatattca atactggaaa tattttccat ctgatgatat
      481 gactgagtat ggacatgaag aaaaccgccg ccgaatcttt taaaaacctc gttggtatac
      541 ggcacgccaa actctttata atgttgcggt gaaagaagcc cggctgcatc ctcgcttaaa
      601 aatgagaggc atctttccat ccacaaaaag ccagctatgt ttccataatt ttcattctta
      661 acattatttt ctatcagttg atatacccat ttcagggctt catcgcagcg ttttatcaat
      721 gtatgtactt tatcagggca ctcgtagaca tcaagaaaaa attcagaacc ccttaaaata
      781 aatgctaatt caagaggaga atgatatccg gatagatata catagcactt attcttcatt
      841 ctttttgtgg catattgaag cccggtaaga gcaatatcaa tgaattcatt gtcctttttc
      901 catgggaagt ttattgtttc ttctattgtt tttccaacag gttctatcca ggatgtgttt
      961 gcaccaaacc tcaaggagcc aagaatcata cccgcaaata ttccggagcc ataccgtaaa
     1021 gcaagtactg gtattgaatc gtcagggtaa tcacttctgt atctgtaaag tttttcttcc
     1081 ctatcacaaa gaagtttcgg aagtaataat acattttttt tgaaatcaat ttcttcaagt
     1141 tgaggaactt tgactgaatt tacaatttct tcttcttctg gcaaaaaagt gtaaatgaga
     1201 caaccctttt ttgctgattg aagtttttta tgcctttcaa gagtttcagc aaaattcttc
     1261 ttaaattcaa aatcattagt actcatgatt accccttatc aattcattga caatggtaac
     1321 gtaaatggct gcactccact gataatcggt gattgttctt gtccagccct tt
//
LOCUS       DAORYU010000336         1365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_65883_length_1365_cov_2.766412, whole genome shotgun sequence.
ACCESSION   DAORYU010000336 DAORYU010000000
VERSION     DAORYU010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65883_length_1365_cov_2.766412"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..871
                     /locus_tag="PLW07_07825"
     CDS             122..871
                     /locus_tag="PLW07_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOL50479.1"
                     /translation="MAKIGLQLYSVRADCAKDLVGTLKKISEMGYEGVEFAGYHGRDA
                     KELRTILQDLHLVCCGTHIGLETLLGEQLLNTVEFNAVLGNKYLIVPGLKEEYRNSIS
                     AWENTAEIFNQVAERLKPYGMKTGYHNHWIEFEMLEGKKPMDVFFQKASKDVIMQFDT
                     GNAMRTGGDVIEYLKKFPGRSDTIHIKEFSASNERAIIGEGDINWKQVIELCQKIGNT
                     QWYIIEQESYAYTPIECVEKCLVNFKKILGV"
     gene            935..>1365
                     /locus_tag="PLW07_07830"
     CDS             935..>1365
                     /locus_tag="PLW07_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOL50480.1"
                     /translation="MAKLAIEGGEKINNKPFPMWPAFSEKTIERALEPLKTGKVSYWT
                     GPYGTQFEEAWAKWNGAKYAITTTNGTSALHTAVAALGIGPGDEVICPSYSFIASSFC
                     VPQAGALPVFADVDQSHTLDPKGIEQHINERTKAIIVVHLYG"
BASE COUNT          451 a          241 c          316 g          357 t
ORIGIN      
        1 cttgaaagtg gatattttcg tatagattct ttaatgaggc aattcgtctt gttgaaaaaa
       61 attacgagac atagtacact gtatcctgaa tatgcaacca acatctacaa agggagtgaa
      121 aatggcaaaa ataggacttc aattgtactc tgtacgggca gattgtgcaa aagaccttgt
      181 tggtacactc aagaagattt cagaaatggg ttatgagggt gtggaatttg caggatatca
      241 tggcagggat gcaaaggaat tgagaacaat tcttcaggat ttacaccttg tgtgctgtgg
      301 tacccatatc ggacttgaaa cccttcttgg agaacaactg ctgaatacag tggagttcaa
      361 tgctgtcctt ggcaataaat acctgattgt gccaggattg aaagaagaat atcgtaattc
      421 tatttctgcc tgggaaaaca ctgctgaaat tttcaatcag gttgctgaac ggctcaaacc
      481 atatggaatg aaaacaggtt atcacaatca ctggatagaa tttgaaatgc ttgaaggaaa
      541 aaaaccgatg gatgttttct ttcaaaaggc atcaaaggat gtcatcatgc aatttgacac
      601 aggaaacgcg atgagaacag gtggcgatgt gattgagtat ttgaaaaaat ttcctggtag
      661 aagcgatacc attcatatta aagaattttc tgcgtcaaat gaaagggcaa ttattggcga
      721 aggtgatatt aactggaaac aggtgattga actttgccag aaaatcggca atacccagtg
      781 gtatattatt gaacaggaaa gctacgcata tacaccgatt gaatgcgtgg agaagtgcct
      841 ggttaatttc aaaaaaatac ttggtgtgta attttgacat agccagagta aacagtacaa
      901 tatactcaac tatcatttaa caatggaggg taatatggca aaacttgcga tagaaggtgg
      961 agaaaaaatc aataataaac cctttcccat gtggcctgca ttttctgaaa agacaatcga
     1021 aagagccctg gaaccactga aaacagggaa agttagttat tggacaggtc cttacggaac
     1081 acagtttgaa gaggcatggg caaaatggaa tggagcaaaa tatgccatca cgacgacaaa
     1141 cggcacaagt gcactgcaca cagcggtcgc agcgcttggc attgggcctg gcgatgaggt
     1201 gatatgtcct tcctattcat ttattgcatc gtctttctgt gtcccccagg caggcgcgct
     1261 gcctgttttt gctgatgttg accagtccca cacccttgac ccaaagggga tagaacaaca
     1321 cattaatgaa agaacaaagg caattattgt agtccatctg tatgg
//
LOCUS       DAORYU010000337         1364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_65934_length_1364_cov_4.991597, whole genome shotgun sequence.
ACCESSION   DAORYU010000337 DAORYU010000000
VERSION     DAORYU010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65934_length_1364_cov_4.991597"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1364
                     /locus_tag="PLW07_07835"
     CDS             <1..>1364
                     /locus_tag="PLW07_07835"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOL50481.1"
                     /translation="YREKLPDSDVYIVYSEQRKISPQKPVGLCFQLKHAFASDIAAEL
                     KNIGFEKSIAVDNRTNVLVVKDTPANIEIVKTIIENLDIEHKNLPVKTTVYSLKYARA
                     GDIWPVVLSVIKKNKGEKDTQSRAEITIGTNTKETGDLGTASAGVSTGVSSSTTGTTT
                     PGTTTGTNTGTSGSYPTLTGQKEKNVSGKVQISTDISAGFSVLPDERTNSLILVGTQD
                     FLDEVTAIIASLDKPALQVSIEAMIVELTEDATKNLGITWTDNSGNLATVTGTFTTAI
                     PGWDTSGTLTWTGLVANVKALQTKGEANILANPRVTTLSGAPATIRITSNIPVAPKVT
                     TTSTGGTGTTVTEYEYRDIGIILVAVPYVGNDNTVTMEVMPKVISAKQSTFFKDAVET
                     SERSTLTKVNVKNGETLVIGGLLSTENIKGENKVPVLGNVFPFLFSNKNRQMKKTDLV
                     IFLT"
BASE COUNT          473 a          235 c          287 g          369 t
ORIGIN      
        1 tatatcgcga aaaattacct gattctgatg tttatattgt gtattccgaa caaagaaaga
       61 tttctccaca aaaacctgta ggtttatgtt ttcaactgaa acatgcattc gcttctgata
      121 ttgctgctga actgaaaaac attggttttg aaaaaagcat tgctgtcgat aatcgaacaa
      181 atgttcttgt tgtaaaagat actccagcga atattgaaat tgtgaagaca attattgaaa
      241 atcttgatat tgagcacaaa aatttgccag taaagacaac tgtatattcc ttgaaatacg
      301 caagagcggg agatatctgg ccagttgtat tgtcagtgat taagaaaaat aaaggtgaaa
      361 aggatactca atcaagggca gaaattacaa ttggcacaaa cacaaaggaa acaggagacc
      421 tcggaacagc gtctgcaggc gtaagcacgg gtgtttcttc atcgacaaca ggaactacta
      481 ctccaggaac aacaactggc acaaatacag gtacatcggg ttcctaccct acccttacag
      541 gtcagaaaga aaaaaatgtt agtggaaaag ttcagatttc tactgatatt tcagcaggtt
      601 tttctgttct tcctgacgaa agaaccaata gtttaatttt agttggaact caggattttc
      661 ttgatgaagt aacagcaatt attgcatctc ttgataaacc agcgcttcag gtatcaattg
      721 aagcaatgat agttgaactg acagaagatg ccacgaaaaa tttaggaata acgtggacag
      781 ataattcagg taatcttgcg acggttacag ggacatttac aacagccatt cctggatggg
      841 atacaagtgg aactttaaca tggacaggtc ttgtcgcaaa tgtaaaggcg cttcaaacaa
      901 aaggagaagc taatatcctt gcaaatccaa gagtaacaac attgagtggc gctccagcaa
      961 caatccgaat tacaagtaat attcctgttg ctcccaaggt tacaacaacc agtacaggtg
     1021 gtactggtac cactgtgaca gagtatgaat acagggatat tggtattatt cttgttgcag
     1081 tcccttatgt tggtaatgat aatacagtga cgatggaagt gatgccaaag gtgatttcag
     1141 caaagcagtc aacatttttt aaggatgctg tggaaacgtc agaaagatca acacttacga
     1201 aggtcaacgt gaaaaatggt gaaacacttg tgattggcgg tcttttgagt acagaaaata
     1261 ttaagggaga aaataaagtg cctgttttag gaaacgtttt tccatttttg ttcagcaaca
     1321 agaacaggca gatgaaaaag acagaccttg tgatatttct tacc
//
LOCUS       DAORYU010000338         1364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_65938_length_1364_cov_3.714286, whole genome shotgun sequence.
ACCESSION   DAORYU010000338 DAORYU010000000
VERSION     DAORYU010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65938_length_1364_cov_3.714286"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="PLW07_07840"
     CDS             <1..422
                     /locus_tag="PLW07_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50482.1"
                     /translation="KQDILKKLMITTALIGAVGLFYLIFLQNVLDAYIQLSRVIRTLI
                     CLLLIFPVGFLMGIPFPSGIRIITQSRKQDIIPWLWTINGLMSVCGSIIAMILAKASG
                     FSSTFFAGTSSYLLLLPLCLILYSKKYHVNFFGGGER"
     gene            419..1345
                     /locus_tag="PLW07_07845"
     CDS             419..1345
                     /locus_tag="PLW07_07845"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HOL50483.1"
                     /translation="MTPEVITVGEILVEIMRKDTTAFDEPGIFMGPYPSGAPAIFISA
                     VSRVSRKKGKAGIIGVVGNDDFGKLVINRLKKDGVDTSCIRRSSQTTGVAFVRYLADG
                     SRQFIFHSGAAGLIEPSDVKKDYFSKARVFHITGSSLFISDTSFQACKKGLEIAVKNN
                     LTVSFDPNIRKEMASFSQNIKKINLFLKHAKILFTTEEEMKILFGNQKIDMAAKKLIA
                     KGTEIVVVKKGEKGSDIYTDSGLIKIPALKIKLVDPTGAGDTYAGAFIACFCQGKNID
                     RCGHIASITASLKCTHQGPMSIPDYQEICQYL"
BASE COUNT          429 a          236 c          283 g          416 t
ORIGIN      
        1 ggaaacagga cattttgaaa aaattaatga taacaactgc tcttattggt gctgtcggtc
       61 ttttttatct tatttttctt cagaatgtgc ttgatgcata cattcaactc tcgcgagtta
      121 tcagaacatt gatatgcctg cttttaattt ttccagtggg atttctgatg gggataccct
      181 ttccttctgg aatcaggatt atcacgcaat ccagaaagca ggacattatc ccatggttat
      241 ggacgattaa tggactgatg tcagtttgtg gctctattat cgcaatgata ttagcaaagg
      301 caagcggttt cagttctact ttcttcgcag gcacttcttc ttatcttttg cttttgcctt
      361 tgtgcctgat tctgtattca aaaaaatatc acgttaactt ttttggcgga ggtgaaagat
      421 gacaccagag gtcattacag taggagaaat tcttgttgaa attatgagaa aagatactac
      481 tgcgtttgat gaacccggaa tttttatggg tccatatcca agtggagcgc ctgcaatttt
      541 tatttctgct gtcagccgtg tatctcgtaa aaaagggaaa gcaggaatca taggtgtagt
      601 gggaaatgat gattttggaa aacttgtgat caatagatta aaaaaagatg gggttgatac
      661 atcctgtata agaaggtcat ctcaaacaac aggcgttgcc tttgtaagat atcttgctga
      721 tggaagccga caatttattt ttcattcagg tgcagcagga ttgattgaac catctgacgt
      781 gaaaaaagat tacttttcaa aagcaagggt atttcatatc acaggtagtt ccctttttat
      841 cagcgataca agtttccagg catgtaaaaa agggcttgaa atcgcagtaa aaaacaatct
      901 aactgttagt tttgacccga atatcagaaa ggaaatggct tcattcagcc agaacattaa
      961 aaaaatcaat ctcttcctga agcatgcaaa aattcttttt accactgaag aagaaatgaa
     1021 aattttattc gggaatcaaa aaattgacat ggctgcaaaa aaacttattg caaagggcac
     1081 agaaattgtt gtggttaaaa aaggtgaaaa gggttctgac atttatactg acagcggatt
     1141 gataaaaata cctgcattga aaatcaaact ggttgaccca actggtgcag gcgacacata
     1201 cgctggtgca tttatcgcgt gtttctgtca gggtaaaaac attgacaggt gtggacacat
     1261 cgcaagtatt acagcatcat tgaaatgcac ccatcagggt ccaatgagta tccctgatta
     1321 tcaggaaata tgtcaatacc tgtaattagt ccagacctat ggct
//
LOCUS       DAORYU010000339         1330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_68474_length_1330_cov_3.791373, whole genome shotgun sequence.
ACCESSION   DAORYU010000339 DAORYU010000000
VERSION     DAORYU010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68474_length_1330_cov_3.791373"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            207..1235
                     /locus_tag="PLW07_07850"
     CDS             207..1235
                     /locus_tag="PLW07_07850"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOL50484.1"
                     /translation="MKEKIQWLDVKEKPIEIKGLAWFEEEKIFRRLPAKSQFQIPEAV
                     DELANHTAGGVARFQTDSRFLKIKVELRAIVNMMHMPATGHSGVDCYIGEPKKQTYCS
                     TAIPLPDNKIYERELFRFNEKKIRSIDLYFPLYNGVNKASIGIENGAKIKKPEPYPVK
                     RRIVVYGTSITQGGCASRPGMAYTNILSRWLGVEFINLGFSGSGKGEPELAHLITMID
                     DVRCIILDYQANAGIEGYISTLPAFVKILREKFRKIPIVIISRPAVAGWFFDRKAKNN
                     YMRGYRFQEKFVRELRAKGDTSIFSYCGRKLFEKMWQESTVDGGHPTDLGFLTMAQAL
                     YPIMKKIM"
BASE COUNT          454 a          207 c          315 g          354 t
ORIGIN      
        1 attcctcttg catctccctt ttataaaggg agaaaaaacc agaaagtagt gtacgagggg
       61 tatctgggaa ggaatttttc tccgtagcgt gcgaaggagg acaatcccct atcaatcggg
      121 tctcgggttc ataaattatg ctggtgtcaa atgttattta aaaagatggt ggaaaggtgt
      181 atcatagtga gaaaacaggg ggatatatga aagaaaaaat acagtggctg gatgtgaagg
      241 aaaaaccaat tgaaatcaaa ggacttgcct ggtttgaaga agaaaaaatt ttcaggcgtc
      301 ttcctgcgaa aagccaattt caaattcctg aggcagtgga tgaacttgcc aaccatacag
      361 caggaggtgt tgccagattt cagacagatt caagattttt gaaaatcaag gtggaactca
      421 gggcaattgt caatatgatg catatgccag caacaggcca tagcggtgtt gactgctata
      481 ttggagaacc aaaaaaacaa acatattgca gtactgcaat tcctttacca gacaacaaaa
      541 tttacgaaag ggaattgttc aggtttaatg aaaaaaagat aagaagtatt gatttgtatt
      601 ttccacttta caatggcgtt aacaaagcga gcattggcat agaaaatggc gcaaaaatca
      661 aaaaaccaga gccatatcca gtaaagagaa gaattgttgt atatggaaca tcaatcacac
      721 agggcggttg cgcaagcaga cctggtatgg catacacaaa tattttaagc aggtggcttg
      781 gtgttgaatt tattaatctt ggtttttcag gcagtggaaa aggagaacca gaacttgcgc
      841 atctgataac aatgatagat gatgtcagat gtatcattct tgactatcag gcaaatgcag
      901 gcattgaagg atacatttct acgttgcctg catttgtgaa gattttaagg gaaaagttca
      961 ggaaaattcc cattgtgatt atatcaaggc cagcagttgc tggatggttt tttgacagga
     1021 aggcgaaaaa taactatatg agaggataca gatttcagga aaagtttgtg agagaactca
     1081 gggcaaaggg agatacaagt attttttctt attgcgggag gaaactgttt gagaaaatgt
     1141 ggcaggaatc aactgttgat ggtgggcatc caacagattt ggggtttttg acgatggcgc
     1201 aggcactgta tccgattatg aaaaagatca tgtgaggcat ctttaatccc tcaggacttt
     1261 ttctaacttc aggtttcctt cctttgcaaa aggaagtcaa ctatataaaa atggacagac
     1321 taaataaaca
//
LOCUS       DAORYU010000340         1325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_68876_length_1325_cov_4.311024, whole genome shotgun sequence.
ACCESSION   DAORYU010000340 DAORYU010000000
VERSION     DAORYU010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68876_length_1325_cov_4.311024"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /gene="uppS"
                     /locus_tag="PLW07_07855"
     CDS             complement(<1..451)
                     /gene="uppS"
                     /locus_tag="PLW07_07855"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HOL50485.1"
                     /translation="MRQIPVHVAIIMDGNGRWAQAKGLPRIAGHKEGVSTVRRVVKVA
                     TDTGVKYITLFSFSTENWKRPPGEVEFLFSLMESTLTQEMKELHDKNIRVRFIGRIWM
                     LPERLQKIIRDTENLTTNNTGLNLTFAVNYGGRQEIIDAFKKAVNSGY"
     gene            complement(448..1164)
                     /gene="lptB"
                     /locus_tag="PLW07_07860"
     CDS             complement(448..1164)
                     /gene="lptB"
                     /locus_tag="PLW07_07860"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011271047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOL50486.1"
                     /translation="MLEIKNISKRFGKRQIIRDVSLQLKKGEIVGLLGPNGAGKTTTF
                     NITMGFLLPDNGQIYLNNTEISRLPVFQRAKLGMAYLFQEPAIFHRLTVIENLVIILE
                     NFVDNREKQRETAIELLKKMGIERLSDNIAGTLSGGEKRRLEIARSLINNPDFILLDE
                     PFSGIDPKTVSEIKSMILDLKKDGVGILITDHNVRDALSITDRAYLIYDGAILIEGKP
                     QEILNDFRSRQVYFGEEFRL"
     gene            complement(1157..>1325)
                     /locus_tag="PLW07_07865"
     CDS             complement(1157..>1325)
                     /locus_tag="PLW07_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016198560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HOL50487.1"
                     /translation="IAVGEEPDTIEEVYESYLVKEGFISRTPQGRVANEKAYQHLNIR
                     TKKPPGLFSDA"
BASE COUNT          363 a          310 c          214 g          438 t
ORIGIN      
        1 aatagcctga atttacagcc tttttgaaag cgtcaataat ctcctgcctg cctccatagt
       61 taacagcaaa cgtcagattc aatcctgtgt tattcgtggt caaattttct gtatccctga
      121 taatcttttg caatctttca ggtagcatcc agattcttcc tataaatctc accctgatat
      181 ttttatcatg caattctttc atttcctggg tcagtgttga ttccatcaga gaaaaaagaa
      241 attccacctc tccaggtggt cttttccagt tttctgtgga aaaagaaaaa agagtaatat
      301 atttgacgcc tgtatccgtt gcaactttca caacccttct cacagtagaa actccctcct
      361 tatgtcctgc tattctcggt aatccctttg cctgagccca tctgccattt ccatccataa
      421 taatcgcaac atgcaccgga atctgcctca taatcgaaac tcctcgccga agtaaacctg
      481 acgacttcta aaatcgttca gaatttcctg tggttttccc tctattaaaa tagcaccatc
      541 ataaatcaaa tatgccctat cggtgatgct gagagcatcc cttacattgt ggtcagtaat
      601 aagaatgccc acaccgtctt tttttaaatc aagaatcatg gacttgattt ctgaaacagt
      661 ttttggatcg ataccggaaa atggttcatc aagaagaata aaatcaggat tattaataag
      721 gcttcttgct atttcaagcc gcctcttttc tccacctgaa agagtccctg cgatgttgtc
      781 tgaaagacgc tctatcccca tttttttcaa caattctatt gctgtctctc tctgtttttc
      841 gcgattgtca acaaagtttt caaggataat gacaagattt tcaatcacag tcaatctatg
      901 aaaaattgct ggttcctgaa aaaggtatgc cattccaagc tttgccctct gaaaaacagg
      961 aagtcgagaa atttccgtgt tattcaaata aatctgtcca ttatcgggaa gcaaaaaacc
     1021 catggtaatg ttaaatgttg tggttttccc ggcgccgttg ggacccagta gtcccacaat
     1081 ttcacccttt ttaagttgaa gggaaacatc tctgataatt tgccttttcc cgaatctctt
     1141 actgatattt tttatttcaa gcatctgaaa ataatcctgg cggttttttt gtcctgatat
     1201 tcaaatgctg gtatgccttt tcgtttgcca ccctgccctg cggagttctg ctgataaaac
     1261 cttccttcac aagatatgac tcataaactt cttcgattgt gtcaggttct tctccaacag
     1321 caatt
//
LOCUS       DAORYU010000341         1307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_70251_length_1307_cov_4.940895, whole genome shotgun sequence.
ACCESSION   DAORYU010000341 DAORYU010000000
VERSION     DAORYU010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70251_length_1307_cov_4.940895"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="PLW07_07870"
     CDS             complement(<1..305)
                     /locus_tag="PLW07_07870"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HOL50488.1"
                     /translation="MNRNNGCKKGIALIELIIFIVIFLIIAGISFRLVYSVNQKAKIV
                     KAKSEITQFAIALENMKDDTGYYPVRLGAIFDENPPAGMERNWNGPYATKMNDMVND"
     gene            complement(365..1042)
                     /locus_tag="PLW07_07875"
     CDS             complement(365..1042)
                     /locus_tag="PLW07_07875"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HOL50489.1"
                     /translation="MKMVKLVMFFVVITILASTGFSATVSVPDTKASPSSTVQVPVEI
                     DNASGIAGFQMTIVFDQNVLTAQQALTGTLTDDWLLFYNVETPGVISIAGLNPGLDEL
                     PEIDGSLVFISFFVEGAVGETTNITITQTGFYNSLAENITVFCQSGLFTIDVNLGDIN
                     GDDTIDISDVILCLRMAIELPVNVNAQNYSSPYPLWLKSRADMNSDTEINISDVILIL
                     RKAIGLD"
     gene            complement(1054..>1307)
                     /locus_tag="PLW07_07880"
     CDS             complement(1054..>1307)
                     /locus_tag="PLW07_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50490.1"
                     /translation="LNQMDISFATSYDDNNLYVIAYDVFDQGEGGELVPYPDGGYFWS
                     EGSPSIVKRVNVFLFPWIMHEKEELLPPYCRQQELPPDE"
BASE COUNT          387 a          282 c          237 g          401 t
ORIGIN      
        1 tcatcattga ccatatcatt catttttgtt gcatagggac cattccagtt tctttccatt
       61 cctgctggcg gattttcatc aaaaattgct cccagtcgta caggataata tcctgtgtca
      121 tctttcatat tctcaagggc aatggcaaac tgggtgattt ctgattttgc tttcacgatt
      181 tttgcttttt gattgactga atagacaaga cggaaactga tgcctgcgat gatcaggaaa
      241 atcacaatga agataatcaa ctcaatcagc gcaatacctt ttttgcatcc gttgtttctg
      301 ttcacatata tgttttaaca cctgattgaa aaaaaatcaa atacagtgtt gatgtcaggg
      361 gaaatcaatc cagtcctatg gcttttctta atatcagaat gacgtctgaa atattgattt
      421 ctgtgtcact gttcatatct gcgcggctct tcagccacaa aggatatggc gacgaatagt
      481 tttgagcatt cacatttaca ggcagttcaa ttgccattct caaacataga atgacatctg
      541 aaatgtctat cgtgtcatca ccattgatat caccaagatt cacatcaata gtgaacaatc
      601 ctgattgaca gaaaacagta atattttctg caagcgaatt gtaaaagcca gtctgggtga
      661 ttgtaatgtt tgttgtttct ccaactgctc cttccacaaa aaatgaaatg aaaaccagag
      721 aaccatctat ctctggcagt tcatcaaggc ctggattcaa tccagcgata cttattacac
      781 caggggtctc aacattgtaa aaaagaagcc agtcatctgt caatgtgcct gtgagtgcct
      841 gttgcgctgt gagaacattc tggtcgaata caattgtcat ctgaaatcct gcaattccag
      901 acgcattgtc tatttcaact ggcacctgca cagtgcttga aggacttgct tttgtatcag
      961 gaacagatac agttgctgaa aaaccagtag aggcaagtat cgtaattacc acaaaaaaca
     1021 tcaccagttt taccattttc atttttcaca ctcctattca tcaggcggca attcctgctg
     1081 cctgcaatac ggtggaagca attcttcttt ttcgtgcata atccatggga aaaggaaaac
     1141 gttcactctt ttcacaatag aagggcttcc ctcactccag aaatatccgc catctgggta
     1201 gggtaccagt tctccacctt ctccctggtc aaagacatca taagcaataa catataaatt
     1261 gttgtcatca taagaggttg cgaaagatat atccatctga ttgagtt
//
LOCUS       DAORYU010000342         1302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_70663_length_1302_cov_6.324779, whole genome shotgun sequence.
ACCESSION   DAORYU010000342 DAORYU010000000
VERSION     DAORYU010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70663_length_1302_cov_6.324779"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1067
                     /locus_tag="PLW07_07885"
     CDS             <1..1067
                     /locus_tag="PLW07_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50491.1"
                     /translation="QLLSRGLILMYPQASKHPQGKIPSWWLMPIYCGYGDQVGIMFEL
                     EGGNGPESRALAYCIQGLYERWINILDQEHVPFGTIIIDAGWSPGGVWVPNEIQWPDL
                     RGFIDRQHKKGRKVLLWIATWFMEGLPDRWCVFCGDRKLVANPENPAYINFIKNNIRK
                     LLSDAKGCYNADGFKIDQLAYAPTERIPLGGYHFGRQVKIGKHHSPVQHNGKRWGCEL
                     LYKLQKEIYLAAKNTKKDCLITSSTVHPYFYDTFDMVRLHDAGVILPETDVFMAMKAR
                     AGLAKAALPAHPVDSDDWVHSYYDKWLDFTTRSKNIGVPCIFYAERFVAIRPVPKILP
                     IKRSDLRTIAKSWRNYIKNL"
     gene            complement(1077..>1302)
                     /locus_tag="PLW07_07890"
     CDS             complement(1077..>1302)
                     /locus_tag="PLW07_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HOL50492.1"
                     /translation="IRNILSENSVNFDIETLLKKACRRSTMAGEKLSETEAAGLRDQL
                     IKCKNPLTCPHGRPTIVEIEKKFIEKQFIR"
BASE COUNT          373 a          228 c          293 g          408 t
ORIGIN      
        1 atcaacttct ttcacggggt ctaatcctga tgtatccgca ggcatcaaag catccacagg
       61 ggaaaatacc ttcgtggtgg ttgatgccca tttattgtgg ttatggtgat caggttggaa
      121 tcatgtttga acttgaaggt ggaaatggac ctgaatcaag ggctcttgcc tattgtattc
      181 agggccttta tgaaagatgg atcaatattc ttgaccagga gcatgtacca tttggcacaa
      241 tcattattga tgctggctgg tcaccaggcg gtgtctgggt tccaaatgaa attcaatggc
      301 ctgatttgcg cggttttata gatagacagc acaaaaaagg gagaaaggtt cttctttgga
      361 ttgcgacatg gtttatggaa ggtttgcctg acagatggtg tgttttttgc ggcgacagga
      421 aactggttgc aaatcccgaa aatcctgctt acataaattt cataaaaaat aatatcagaa
      481 aacttctgtc tgatgctaaa ggatgctaca atgccgatgg atttaagatt gaccagcttg
      541 catatgctcc gacggaaagg atacctcttg gaggatatca ttttggaagg caagtgaaga
      601 taggcaaaca tcattcccct gttcaacata atggaaaacg atggggttgt gaattattgt
      661 ataaacttca aaaagaaata tacctggctg cgaagaatac caagaaggat tgtttgatta
      721 caagtagtac tgtccatcca tatttttatg atacttttga tatggtgaga ttgcatgatg
      781 ctggagtaat attgccagag acagacgttt ttatggctat gaaagcgcga gccgggcttg
      841 caaaggcagc actaccagcg catcctgttg acagcgatga ttgggtgcac tcttattacg
      901 ataaatggct tgatttcaca acgagaagca agaatattgg tgttccctgt attttttatg
      961 ctgagaggtt tgttgcaata agacctgtac cgaaaattct gcccatcaaa aggtctgact
     1021 taagaactat tgctaagtcg tggagaaatt acatcaaaaa cctttagaat ttttgactat
     1081 cttatgaatt gtttttctat aaattttttc tcaatttcaa caattgttgg cctgccatgg
     1141 ggacaggtca gcgggttttt gcatttgata agttgatccc ttaatcctgc agcttcagtt
     1201 tcagaaagtt tttctccagc cattgttgac cttctgcatg ctttcttcaa aagagtttca
     1261 atgtcaaaat ttacactgtt ttctgaaaga atatttctga tg
//
LOCUS       DAORYU010000343         1299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_70905_length_1299_cov_6.184887, whole genome shotgun sequence.
ACCESSION   DAORYU010000343 DAORYU010000000
VERSION     DAORYU010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70905_length_1299_cov_6.184887"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="PLW07_07895"
     CDS             complement(<1..218)
                     /locus_tag="PLW07_07895"
                     /inference="COORDINATES: protein motif:HMM:NF024912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50493.1"
                     /translation="MCGICGIARKEKRVSETLIRKMASTMVHRGPDEEGFQLFDNTGL
                     GHRRLKIIDLTTGKQPMSNENNTIFLIC"
     gene            complement(234..>1299)
                     /locus_tag="PLW07_07900"
     CDS             complement(234..>1299)
                     /locus_tag="PLW07_07900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOL50494.1"
                     /translation="IGTLEVGGTEKQLYLLLKNMDKSKFSPFVISLRDGRMRNDFQQI
                     STLYILNKKYKFDVTSFLTLVKVILKEKPDIVHTFLFTSNTWGRLAAILTGVPVIIAS
                     ERSMDIWKKKFHFFIDTVLGFFTDAIICNSIVVKEFYEKKLGPVSRKLTVIRNGIEWQ
                     QFNIENQAHPKNEKIIFTASRLAPEKGIEYLIQAIKILSARMNNMKLLVAGEGPLKTR
                     LEYLVEQQGIKEIVTFLGYQENVSKYISQADVVVLPSLWEGLPNILLEAMAMKKPVVA
                     TAVGGTKEIVRDGENGFLVRPKDAKALAEAIQKILQDINLAINFGESGYRFVRENFDL
                     SLMVSSYEKLYQKLLCRQMI"
BASE COUNT          380 a          275 c          191 g          453 t
ORIGIN      
        1 ttgcatataa gaaaaatggt attgttctca tttgacatcg gttgtttgcc tgtggtgaga
       61 tctataattt tcaatcgtct gtgtcctaac ccagtattat caaacaattg aaaaccttct
      121 tcatctggtc cgcgatgaac cattgtggat gccatctttc ttatcagtgt ctctgaaact
      181 cgtttttctt ttcgagcaat tccgcatatt ccgcacattt ttacctcgct atatcaaatc
      241 atctgtctgc ataacaactt ctgataaagt ttttcgtaag aagaaaccat cagggataaa
      301 tcaaagtttt ctcttacaaa tctgtaaccg ctttcaccaa aatttattgc aagattgata
      361 tcctggagaa ttttttggat ggcttctgca agtgctttag catcctttgg tcttacaaga
      421 aaaccatttt ctccatccct aacaatctct tttgttccac caaccgctgt tgcaaccact
      481 ggttttttca ttgccattgc ttcaagaaga atatttggca atccttccca taaggaaggc
      541 aaaacaacga catctgcctg tgaaatatac ttgctgacat tttcctgata tcccagaaat
      601 gtcacaattt cttttattcc ctgttgctca acaagatact caagccgtgt tttcaagggc
      661 ccttcgccag caactaacag tttcatgtta ttcattcttg ccgacaaaat ttttattgcc
      721 tgtatgagat attcaatacc tttttctggc gcaagtctac tcgcagtaaa aatgattttc
      781 tcatttttgg gatgtgcctg attttctata ttaaattgtt gccattcaat gccatttcgt
      841 atcacagtta attttcttga aacaggtcca agttttttct cataaaattc ttttacaaca
      901 atcgaattgc atatgatagc atccgtaaaa aaacccaaaa cagtatcaat aaaaaaatga
      961 aattttttct tccagatatc catacttctt tcagaagcaa tgatcacagg aacccctgta
     1021 agtattgctg caagacgacc ccatgtattt gatgtgaaca aaaacgtgtg aacaatgtca
     1081 ggtttttctt tcagtatcac cttcaccaga gtcaaaaagc ttgttacgtc aaatttatat
     1141 tttttattca aaatatacag ggtcgaaatt tgctgaaaat cgtttctcat tcttccatct
     1201 ctcaaagaaa taacaaatgg tgaaaattta ctcttgtcca tatttttgag taaaagatac
     1261 agttgttttt ctgtcccacc gacttccaat gtaccgatg
//
LOCUS       DAORYU010000344         1298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_70990_length_1298_cov_3.576830, whole genome shotgun sequence.
ACCESSION   DAORYU010000344 DAORYU010000000
VERSION     DAORYU010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70990_length_1298_cov_3.576830"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="PLW07_07905"
     CDS             <1..763
                     /locus_tag="PLW07_07905"
                     /inference="COORDINATES: protein motif:HMM:NF036579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOL50495.1"
                     /translation="LKKKIATFYWSKEKQAFKKYRKNSSAKPLAASENIIGQHENFLF
                     NLLEIGSEDQRKLAMNNIAGICGMYLPNLGDYQSAFIPEHHGNYVGEEVIKIGSPFWS
                     FYAILSLIKSKKYKEALNYIRTCWGIMLEFDATGCWEMWDRHTSFCHGWSAAPGMILP
                     AYAGGIRPIEPGFKKFLVEVVLCDFEWVKADVPTPYGIISVSWNMKKQKVFEISVKVP
                     ESTTGVLVPPPGFGPRKKMILKSGLHKFTFKRRKN"
     gene            760..>1298
                     /locus_tag="PLW07_07910"
     CDS             760..>1298
                     /locus_tag="PLW07_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50496.1"
                     /translation="MTDRERVKRILHYENYDRMPLVHFGFWNETLNLWACEGHIPEEI
                     AKNWADGNYADSEIAEIIGFDFNWYNVFKPILGLWPAFESKVIEELPDGTKKILNRDG
                     VVTLHKPGAGSIPADVDHLLKDRTSWEKLYLPKLQWSKERVLKAPVIAGRDVLTFEQG
                     GLNYLLTQERTKPLGIYCG"
BASE COUNT          432 a          215 c          290 g          361 t
ORIGIN      
        1 actgaaaaaa aagatagcaa cgttttactg gagtaaagaa aaacaggcat tcaaaaagta
       61 tagaaaaaat agttccgcga aaccactggc tgcatcagaa aatatcattg gtcaacacga
      121 aaatttcctg tttaatctac ttgaaatagg ttcagaagac cagagaaaac ttgcaatgaa
      181 caacattgct ggaatttgcg ggatgtatct tccaaatctt ggtgattacc agagtgcttt
      241 cattcctgaa caccacggaa attatgtggg tgaagaagtg ataaaaattg gaagcccttt
      301 ctggtctttc tatgctattt tgtctttgat aaagtcaaaa aaatacaaag aagcactgaa
      361 ttatattcgc acatgctggg gtattatgct tgaatttgat gcgacaggtt gctgggaaat
      421 gtgggatagg cacacctctt tttgccatgg ctggagcgct gctccaggca tgatacttcc
      481 tgcttatgct ggcggtatca gaccaataga accaggtttt aagaaatttc tggttgaagt
      541 tgttttatgc gattttgaat gggtaaaggc agatgtacca acgccatacg ggattatttc
      601 tgtatcatgg aacatgaaaa aacagaaggt ttttgaaata tctgtgaagg tgcctgaatc
      661 aacaacagga gtacttgttc cacctcctgg tttcggtcca aggaaaaaga tgatattaaa
      721 atcaggactg cataaattta ctttcaaaag gagaaaaaat tgactgacag agaaagagta
      781 aaaagaattc ttcactatga aaattatgac aggatgccac tggtacactt tggtttctgg
      841 aatgaaacct taaatttatg ggcttgtgag ggacatatcc ctgaagaaat tgcaaaaaac
      901 tgggctgatg gaaattatgc agatagcgaa attgcagaaa ttattggatt tgatttcaac
      961 tggtacaatg tgttcaaacc aatacttggt ctctggcctg cttttgaatc aaaggtcatt
     1021 gaagaacttc ctgatggaac aaagaaaatt ctaaatagag atggggtggt caccctccac
     1081 aaaccaggtg ctggttctat tcctgctgat gttgaccatc tgctgaaaga caggacttcc
     1141 tgggaaaaat tgtacctgcc gaaattacag tggtcaaaag aaagagtatt aaaagcgcct
     1201 gtcattgctg gcagagacgt attgaccttt gaacagggtg gattgaatta tcttttgaca
     1261 caggaaagaa ccaagccatt aggaatttat tgtggaag
//
LOCUS       DAORYU010000345         1286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_71944_length_1286_cov_5.219334, whole genome shotgun sequence.
ACCESSION   DAORYU010000345 DAORYU010000000
VERSION     DAORYU010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71944_length_1286_cov_5.219334"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..>1286)
                     /locus_tag="PLW07_07915"
     CDS             complement(184..>1286)
                     /locus_tag="PLW07_07915"
                     /inference="COORDINATES: protein motif:HMM:NF020048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50497.1"
                     /translation="SLLEEIDDIEVIPVASEGEALVLECSLIKKYQPGYNVDLKDGKT
                     YPWLKITDDIFPAVMVVREQKKPGPQYFGPFTDVKGLKQVLRFIRKYYPVRTCRKRII
                     EGKTSKPCTFFFTKRCCGPCTGNVKPEEYKKIIDGIKAFFSGKYKNYCKELKKQLNEV
                     IRNWDFEQAKLIAERIHLLERMQERLPWRSEDELLLYRRENVLPAIAEKLLLGKIPAV
                     IEGYDISHFGGSHATGSRVVFKGGVQSRADYRKFRIKTVEGIDDYSMLREVIRRRFTD
                     PEMQARPDLILVDGGKGQLNEVLEELKKLKLNIPVVALAKKEEIVYSSLSKKAIFLPK
                     NSSELHLLQDIRNEAHRFARSYHSKLRTKEIS"
BASE COUNT          359 a          275 c          199 g          453 t
ORIGIN      
        1 tatcagaaat atcctttgtg gacaggccca tatcagttta ttgcagaagg aaatgagttt
       61 attgacgatt atcagattgt gccctgcgga ctgatgaaag atattgagat tatcacatac
      121 cagatttcat agtttgtttc tataattacg gttgaaactg gaatttgtta acacttctgc
      181 catttatgat atttctttag tgcgaagctt ggaatgatag gaacgtgcga aacgatgggc
      241 ttcgttcctg atatcctgaa gaaggtgaag ttcacttgaa tttttcggaa gaaaaattgc
      301 cttcttactc aagcttgaat acacaatttc ttcttttttg gcgagcgcaa ccacaggaat
      361 attcagtttc aatttcttca gttcttcaag cacttcgttc aattgtcctt taccgccatc
      421 aacaaggatt aaatcaggtc ttgcttgcat ttctggatca gtaaatcttc ttcttatcac
      481 ttccctgagc atactataat catcaattcc ttccacagtt ttaattctaa atttcctgta
      541 atctgcccta ctctgcactc cacccttgaa tacaaccctg cttccagtgg catggcttcc
      601 accaaaatgt gaaatatcgt atccttcaat cactgctggt atttttccaa gtaaaagttt
      661 ttctgcaata gcaggcaata cattttctct tctataaaga agaagttcat cttcacttct
      721 ccagggaaga cgttcctgca tcctttcaag cagatgaatt ctttcagcaa tcaattttgc
      781 ctgctcaaaa tcccagtttc ttattacttc attcaactgt ttttttaatt ccttgcaata
      841 gtttttgtat ttgcctgaga aaaatgcctt aattccatca ataatttttt tatattcttc
      901 tggttttaca ttaccggtac agggaccaca acagcgcttt gtaaaaaaaa acgtacatgg
      961 ctttgacgtt tttccttcaa taattctttt cctgcaggtt ctcacaggat aatattttct
     1021 tataaaccgt aatacctgtt tcaatccttt cacatctgtg aatggaccaa aatactgggg
     1081 tcctggtttt ttttgctctc ttacaaccat aacagcagga aatatgtcat ctgtaatttt
     1141 taaccacgga taggttttcc catccttcaa atcaacatta tacccgggtt gatacttttt
     1201 tatcagagag cattcgagaa ccaatgcctc accttctgat gcaacaggaa ttacttcaat
     1261 atcgtcaatt tcttcaagca atgaag
//
LOCUS       DAORYU010000346         1278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_72575_length_1278_cov_5.898610, whole genome shotgun sequence.
ACCESSION   DAORYU010000346 DAORYU010000000
VERSION     DAORYU010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72575_length_1278_cov_5.898610"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1244
                     /gene="glnS"
                     /locus_tag="PLW07_07920"
     CDS             <1..1244
                     /gene="glnS"
                     /locus_tag="PLW07_07920"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HOL50498.1"
                     /translation="IEENLDLFRRMREGEFPEGSKTLRAKIDMASPNLNLRDPVMYRI
                     IKKIPHYRTGDKWYIYPSYDWAHGLEDSIEGITHSLCSLEYENHRPLYDWFLDQLGVH
                     HPRQIEFARLNMTYTVLSKRYLNELVKKSFVRGWDDPRMPTLAGMRRAGYPPEAIRKF
                     CNAIGVAKTDSVIDIELLEHFVREHLNKVCLRKMVVLNPIKLVIENYPKGKVEYLEAI
                     NNPENSDDGIRIVPFCRELYIDQQDFMENPVPGFYRLAPGREVRLRYAYFIRCTDIEK
                     DSEGKITLIKCTYDPATRGGNCPDKKRVKATLHWVSALHCIDIEVRLYDRLFLKKNPL
                     EFPEGADFTCNINPDSLKIAMAKAEPSLKEAKVGDRFQFERIGYFACDPDSTPEKIVF
                     NRTVSLKDEWEKIRKFLNMQA"
BASE COUNT          409 a          237 c          280 g          352 t
ORIGIN      
        1 gtattgaaga aaatcttgac cttttccgga gaatgaggga aggtgaattt ccagaaggtt
       61 caaaaaccct cagggcaaaa attgatatgg catccccgaa tctcaattta agagaccctg
      121 tgatgtacag aattattaaa aagattcctc attatcgaac aggtgataaa tggtacattt
      181 atccgtctta tgattgggca catgggcttg aagattcaat tgaaggaatt actcattccc
      241 tctgttctct tgagtatgaa aaccataggc ctctgtatga ctggtttctc gaccagctgg
      301 gtgttcacca tccaaggcag attgaatttg cgcggctcaa tatgacctac actgttttga
      361 gcaaaagata cctcaatgaa ctggtaaaaa aaagttttgt aaggggatgg gatgatccgc
      421 gtatgccaac tcttgcagga atgagaagag caggttatcc tccagaagca atcagaaagt
      481 tctgcaatgc cattggagtg gcaaaaacag acagtgtcat tgatatagaa cttcttgaac
      541 attttgttag agaacatttg aacaaagtgt gcctgagaaa aatggtggtt ttgaatccaa
      601 taaaactggt cattgaaaat tatccaaaag gcaaagttga atatctggaa gccatcaata
      661 atcctgaaaa ttctgacgat ggcataagaa tagttccatt ttgccgggaa ctttatattg
      721 atcaacagga tttcatggaa aacccagtac cagggtttta ccgacttgct ccaggaagag
      781 aagtcagact tcgttacgcg tactttatca gatgcacaga tattgagaag gattcagaag
      841 gcaaaattac tctcataaag tgtacatatg accctgcgac cagaggcggt aattgccctg
      901 ataaaaaaag ggtcaaagca acattgcact gggtttctgc actacattgt attgacattg
      961 aggtacgact ctatgaccgg ctatttttga aaaaaaaccc gctggaattt cctgaaggtg
     1021 ctgattttac ttgcaacatt aatccagatt ctttaaagat tgcaatggca aaggcagaac
     1081 catctttgaa ggaggcaaag gttggagaca ggtttcagtt tgaaaggatt ggctattttg
     1141 cgtgcgaccc tgattcaact ccagaaaaga ttgttttcaa ccggactgtt tccttgaagg
     1201 atgagtggga aaaaattaga aagttcctca acatgcaggc ctgattttac agaagattcc
     1261 ccatgccttt ccaccatt
//
LOCUS       DAORYU010000347         1277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_72646_length_1277_cov_4.950900, whole genome shotgun sequence.
ACCESSION   DAORYU010000347 DAORYU010000000
VERSION     DAORYU010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72646_length_1277_cov_4.950900"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="PLW07_07925"
     CDS             complement(<1..731)
                     /locus_tag="PLW07_07925"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOL50499.1"
                     /translation="MKLKIAVAGFRHGHIYDIIARAKNHPETAIVACCEEDEKTRMFI
                     EQQGIAKITHTDCMKMIEDVDCDIIAIGDCYGKRGKIAIESLKKGRHVLADKPLCTRV
                     SEIKEIIKISQEKNLKVGCQLTMRDMPQFIELRNLIQSGKLGEIHSIIFTGHHPLMIN
                     ERPTWYFEPDMHGGTINDIGVHAVDYIEWATGLQISTINAARSWNAFAKKYPHFMDAG
                     QMMLTLDHGCGVLGDVSYFAPDSHG"
     gene            complement(746..>1277)
                     /locus_tag="PLW07_07930"
     CDS             complement(746..>1277)
                     /locus_tag="PLW07_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50500.1"
                     /translation="RIYRQFSSEWQPAVLDHELSSCEPCFSWNIFHSLQLEDRERFTM
                     GLYSLFAAGASRQSFVSCETRNGIFGNCFSYGLALILTRLCVIQEKDNQIHLMKMAPV
                     AFFEGNGLNWKNVPTHSGNISISARFYDKIFNVSVAKSGKKVETIVHIPPLQQLEYLI
                     INKKKVKYTGQKFFTI"
BASE COUNT          355 a          275 c          221 g          426 t
ORIGIN      
        1 tatccatgac tatcaggtgc aaaataactg acatctccaa gaactccgca accatgatcc
       61 agtgttaaca tcatctgtcc tgcatccata aaatgcggat atttttttgc aaaggcattc
      121 catgaccttg ctgcattgat tgttgaaatt tgaagacctg ttgcccattc aatgtaatca
      181 acagcatgaa ccccgatatc attgatggtg ccaccatgca tatcgggttc aaaataccat
      241 gttggtcttt cattgatcat caggggatgg tgccctgtaa aaatgatact gtggatttca
      301 cccaacttgc ctgattgtat cagattgcga agctcgataa actgcggcat gtctctcatc
      361 gtcaactgac acccaacttt caagtttttt tcttgagaaa tttttatgat ttctttgatt
      421 tcagacacac gcgtacacag tggtttgtct gctaaaacgt gcctgccttt tttcaatgat
      481 tcaatggcaa tttttcctct tttcccataa cagtcgccaa ttgctataat atcgcaatct
      541 acatcttcaa tcattttcat gcaatcagtg tgggtgattt ttgcaatacc ctgctgttca
      601 ataaacattc ttgttttttc gtcttcttca cagcatgcca caatagcagt ttctggatga
      661 tttttcgctc ttgcaataat atcgtaaatg tggccatgcc tgaatccagc aactgcaatc
      721 ttcagtttca atctttctcc cttttttata ttgtgaaaaa tttctgacct gtgtatttta
      781 cctttttttt gtttattatc agatattcga gttgctgtaa gggtggaata tgaacaatgg
      841 tctccacctt tttacctgac tttgcaacag aaacattgaa aattttatca taaaatcttg
      901 cagaaattga gatatttcca gagtgtgttg ggacattttt ccagttcaat ccatttcctt
      961 caaaaaaggc aacaggcgcc attttcatta ggtgaatctg gttatctttt tcttgaataa
     1021 cacacaaccg tgtaagaatc agggcaagcc cataggaaaa gcagttcccg aaaataccat
     1081 ttcttgtttc acatgaaaca aaactctgcc ttgatgcgcc agcagcaaaa agactgtata
     1141 gccccattgt aaatctttcc ctgtcttcca gttgaaggga atggaagatg ttccatgaaa
     1201 aacatggttc acaagaagaa agttcgtggt ccagaactgc tggctgccat tcgcttgaaa
     1261 attgtctgta aattctc
//
LOCUS       DAORYU010000348         1276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_72730_length_1276_cov_4.833743, whole genome shotgun sequence.
ACCESSION   DAORYU010000348 DAORYU010000000
VERSION     DAORYU010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72730_length_1276_cov_4.833743"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="PLW07_07935"
     CDS             <1..650
                     /locus_tag="PLW07_07935"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOL50501.1"
                     /translation="VEFPDEKERLIKVYLPVSVEVDSISFSADGEPLKKPEQRLLFFG
                     DSITQGMDAVSPVCTYPVVCAKILNMDYINQGVGGYVFDAGSLDEDFPIEPDIITVAY
                     GVNDWNKDKSAWEINQAAATYLEKLKSIFKKAHIFVITPIWTDRENQKKASGTLQDLR
                     KNIEQVAKKTGCYVINGLTLVPNNSFYFVDGIHPNETGHLMYGTNLACIIQGQLK"
     gene            669..>1276
                     /locus_tag="PLW07_07940"
     CDS             669..>1276
                     /locus_tag="PLW07_07940"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOL50502.1"
                     /translation="MREIKLLKYGWKFKKEDIPDAVKLNFDDSNWESVRIPHDWAIKG
                     PFSPENDVHTITVVDKTGKKQVDFTGETGGLPHVGKAWYRLKFHLPPDIKTKRVRVEF
                     DGVMSHSKVYCNETYVGSWPYGYSSFAFDITECINPGENLLAVFVDNKPNASRWYPGA
                     GIYRNARLVITNPVCVGHWGTYITTPFVSKEKAKVNVKTTIE"
BASE COUNT          416 a          202 c          299 g          359 t
ORIGIN      
        1 tggtagagtt tcctgatgag aaagaaaggc tgattaaggt ttatttacct gtttcagttg
       61 aggttgatag tatttccttc agtgcagacg gcgagccctt gaagaaacca gaacaacgat
      121 tgttattttt tggagattca attacgcagg ggatggatgc tgtaagtcct gtgtgtactt
      181 atcctgttgt atgtgcgaaa attttgaata tggattatat caatcagggt gtgggtggtt
      241 atgtttttga tgctgggtct cttgatgaag attttccaat tgaacctgat attatcactg
      301 tggcatatgg cgtgaatgac tggaacaaag acaaatccgc ctgggaaata aatcaggcag
      361 cagcaacata ccttgagaaa ctgaaaagta tctttaagaa ggcacacatc tttgttatta
      421 cgccaatctg gactgataga gaaaaccaga aaaaggcatc aggtacactg caggatttga
      481 gaaaaaatat tgaacaggtg gcaaagaaga caggatgtta tgtcattaac ggtcttaccc
      541 ttgtgccaaa taacagtttt tactttgttg atggaattca tcccaatgaa acaggtcatc
      601 ttatgtatgg tacaaacctt gcctgcataa ttcagggaca acttaaataa aattatctgg
      661 agaaaacgat gagggagata aaattattga aatatggctg gaagtttaaa aaggaagata
      721 ttcctgacgc agtaaaatta aattttgatg attctaattg ggaatcagtg agaattcctc
      781 atgactgggc tatcaagggg cctttcagtc cagaaaacga tgtgcatacc attactgtcg
      841 tggataagac aggaaaaaaa caggtagatt tcaccggtga gacaggtggg cttcctcatg
      901 tcggaaaggc gtggtatcgc ttaaaatttc atttaccgcc tgatatcaaa acaaaaaggg
      961 taagggttga gtttgatggg gtaatgagcc atagcaaggt atattgcaat gagacatatg
     1021 tgggtagctg gccatatgga tactcatcct ttgcatttga tatcacagaa tgtataaatc
     1081 ccggtgaaaa tttattggct gtgtttgttg acaataagcc aaacgcttca cgatggtacc
     1141 ctggagcagg aatttataga aatgccaggc ttgtcatcac aaaccctgtg tgtgtgggac
     1201 actggggaac ttacatcaca acaccatttg taagcaaaga aaaagcaaaa gttaatgtga
     1261 aaacaactat tgaaaa
//
LOCUS       DAORYU010000349         1276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_72763_length_1276_cov_2.376740, whole genome shotgun sequence.
ACCESSION   DAORYU010000349 DAORYU010000000
VERSION     DAORYU010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72763_length_1276_cov_2.376740"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="PLW07_07945"
     CDS             complement(<1..420)
                     /locus_tag="PLW07_07945"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HOL50503.1"
                     /translation="MNDALPYTFGGIKQIPLSRAKFVILPVPYESTVSFKPGTRFGPE
                     AIIFSSRFMELYEEETHSEPYLEGILTPGEIVQEIDSAGKMMKKIQNTARKFVEKNCF
                     VFTLGGEHSISFPLVKEHQKKYPDLKILHFDAHADLRI"
     gene            complement(424..972)
                     /locus_tag="PLW07_07950"
     CDS             complement(424..972)
                     /locus_tag="PLW07_07950"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HOL50504.1"
                     /translation="MDFVPKKAFMTKGVGRDREKLTSFERALRNAGIAQFNLVSISSI
                     FPPHCKLISKTEGLSYLKPGQILFVVMSKNATNEPHRLITSSVGLAIPKDPNQYGYLS
                     EHESFGETEEKAGDYAEDLAATMLATILGLEFDPNSSYDEKKQIWRMSNQIIKTTNIT
                     QSAIGDKNGLWTTVIAAAVFVL"
BASE COUNT          364 a          231 c          234 g          447 t
ORIGIN      
        1 aattcttaaa tcagcatgcg cgtcaaagtg aagtattttt aaatcaggat attttttttg
       61 atgttctttg accagtggaa aacttattga gtgttctccg ccaagtgtga acacaaaaca
      121 atttttttct acaaactttc ttgctgtgtt ttgaattttt ttcatcattt tgccagcaga
      181 gtcaatctcc tgaacaattt caccaggtgt gagtatgcct tcaagatacg gttctgaatg
      241 tgtttcttct tcatacaatt ccataaatct tgaagaaaaa atgattgcct caggaccaaa
      301 cctggttcct ggcttaaatg atacagtgct ttcgtagggg accggtaaaa tgacgaattt
      361 tgccctgctt agaggaattt gttttattcc gccaaaggta tagggtagag cgtcgttcat
      421 ttgttataat acgaagaccg cagcagcaat gactgttgtc cataggccat ttttgtcgcc
      481 aattgctgac tgggtaatgt tggttgtttt aatgatctgg tttgacatgc gccagatttg
      541 tttcttttca tcatatgaag agttcgggtc aaattcaagc cctaaaattg ttgcaagcat
      601 cgttgcagca aggtcttcag cataatcacc tgctttttct tctgtctcac caaaactttc
      661 atgttcagag agataaccat actggttcgg atctttcgga atagccagac caactgatga
      721 ggtaattaat ctgtggggtt cgtttgttgc atttttactc attacaacaa acagaatctg
      781 tcctggtttc agatacgaaa gtccctctgt ttttgaaatg agtttgcagt gaggtgggaa
      841 aatacttgag attgaaacaa gattgaattg agcaatacct gcatttctca atgcccgctc
      901 aaaacttgta agtttttctc tgtccctgcc aacccccttt gtcatgaatg ctttttttgg
      961 cacaaaatcc actctttttt ctccttttgg ataatctgta actttctaat taaaaacctt
     1021 tgttaaaatt ttgtcaaatt ttttcatgtg tcaaaaacac tattatgtca aatttgtcga
     1081 ttcttgattc acggggtaaa aaattttttt cttccaaaaa agagatacaa aatctatttc
     1141 tagaaagcga taatattgag tgatattaga catatttaaa gtattatgta attataaata
     1201 gtccttgcag tccaattatt gtactgcccc atttgtcaag agcaaaataa gggagaattc
     1261 agagtcctgg ttaaag
//
LOCUS       DAORYU010000350         1270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_73226_length_1270_cov_4.032922, whole genome shotgun sequence.
ACCESSION   DAORYU010000350 DAORYU010000000
VERSION     DAORYU010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73226_length_1270_cov_4.032922"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1270)
                     /locus_tag="PLW07_07955"
     CDS             complement(<1..>1270)
                     /locus_tag="PLW07_07955"
                     /inference="COORDINATES: protein motif:HMM:NF028479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HOL50505.1"
                     /translation="KSSLDGGPIPEIGDIRGPIAILGPENRIAIATCDRNEILILNNG
                     GSYWKTIGGALPGRAPGQFQFITSLRATTNHLYPVECYDNPRVQIFDVNGNFIAQLGM
                     TAGEFLNPAGITIKNEKVYVCGQREGKIVVYDKQGRFDSEIIIPYRSSDLSLVDLAYA
                     ARPTRIVFDDAGKTYLLDDNHSRIAVLNASGTTVEITISPRDESNYYFKPAGIALVGD
                     YLYVAGNNQQENKGSKVFVYNKNGILQKTFAGTTAYRYKDMAAGNGRLYLLTDDENPY
                     FDLNPGVMVYDTDGNFMATLSEIKNANYLTIDSQEKIFASNWYQGTQRSTFAVFDQQG
                     NYLWHYLSSEPVEATGMAVDSNGNVYFSDGCNHRLHKFSTAENTLAILQCQVDNTPPT
                     AQISFPLHNRDCNFLQNFLVIGTVSDKNLH"
BASE COUNT          350 a          285 c          259 g          376 t
ORIGIN      
        1 tagtgaagat ttttatcgct gactgtgcca atcacgagaa aattctgcag gaaattacaa
       61 tccctgttgt ggagaggaaa acttatctgg gccgtaggtg gggtattatc cacctgacac
      121 tggagaattg ccagggtgtt ttctgcagtt gaaaatttat gaagcctgtg gttgcagcca
      181 tctgaaaagt aaacattgcc attactgtca acagccattc ccgtggcctc aactggctct
      241 gaagacaggt aatgccacaa atagtttccc tgctggtcaa atactgcaaa agtgcttctc
      301 tgcgtgccct ggtaccagtt agaagcaaaa attttttcct ggctgtcaat tgtcagatag
      361 tttgcatttt ttatttcaga cagggtagcc atgaaattac catctgtgtc ataaaccatg
      421 acccccggat tcaaatcaaa atatggattt tcatcatctg taagaaggta aagccgtcca
      481 ttacctgccg ccatatcctt atacctgtac gctgttgtac cggcaaaagt tttttgaaga
      541 ataccatttt tattataaac gaatacctta cttcctttgt tttcctgctg gttatttcca
      601 gcgacataaa gataatcgcc gacaagtgca atgcctgctg gcttgaagta atagtttgat
      661 tcgtctctgg gcgaaatggt tatttcaact gtagttcctg aagcatttaa gaccgctatt
      721 ctactgtggt tgtcatccag aagataagtt tttcctgcgt cgtcaaacac aattctggtt
      781 ggccgcgcag cgtaagcaag gtcaaccagg gaaaggtctg aagacctgta tggaatgata
      841 atctcagaat caaaccttcc ctgcttgtca taaaccacaa ttttcccctc tcgctgacca
      901 cagacataaa ccttttcatt tttgatggtg atgccagcag gattgagaaa ttcgcccgca
      961 gtcatgccca actgggcaat aaagttacca ttgacatcaa aaatttgtac cctgggattg
     1021 tcataacatt ccacagggta caaatggtta gttgtggccc gcaaacttgt aatgaattga
     1081 aattgaccag gagcgcgacc aggcaaggcc ccgccaattg ttttccagta actaccgcca
     1141 ttgttcagaa tcaaaatttc atttctgtca caagtagcaa ttgcaattct gttttccgga
     1201 cccaaaatgg caatgggtcc tctgatatct cctatttctg ggattggacc tccatcaaga
     1261 ctgcttttta
//
LOCUS       DAORYU010000351         1270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_73231_length_1270_cov_3.841152, whole genome shotgun sequence.
ACCESSION   DAORYU010000351 DAORYU010000000
VERSION     DAORYU010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73231_length_1270_cov_3.841152"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="PLW07_07960"
     CDS             complement(<1..833)
                     /locus_tag="PLW07_07960"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOL50506.1"
                     /translation="MNFRERFIASLDFSKPDKVCFIPGGPRESTFKRWHSEGLPENTD
                     YMEVLCSIIGIPYEKQGSSKYISVSFRMNPMFEEKILQHKDGHYIVQDWMGAIVEISD
                     KYDLSYLRAAKDFVTRKWYKFPVETRTDWLEMKKRYDPDDPMRFPENFDELALANKNR
                     TWLQSLGFAGPFWQLRDWCGFEGLCMLMVEDPEFVEEMAEFWKDFVLAVMEKFFAKAT
                     CDHLLISEDMAYKEKSMISPAMAKKFLAPCWKAWSERAQKAGCKIIEVDSDGYIGQLI
                     PV"
     gene            complement(830..>1270)
                     /locus_tag="PLW07_07965"
     CDS             complement(830..>1270)
                     /locus_tag="PLW07_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50507.1"
                     /translation="CCGVRSCEDTTTLLSPEIYKSVYPYFNRSLEQFNGFVHFCGSGA
                     HLLQYFLNCDYVKIINFGNPERFDWNETIKLFVKYGKTYYGTVKRFDGEALPDYFERV
                     LEPLERKSNLMFVPQLLPGEEPEQAIEIWYNVQNKKFYKQKGAQ"
BASE COUNT          381 a          288 c          194 g          407 t
ORIGIN      
        1 acaggtatca actggccaat atatccatca gagtcaacct caataatttt gcatcctgct
       61 ttttgcgctc tttcactcca tgccttccag cacggtgcaa gaaatttttt tgccattgct
      121 ggagaaatca tacttttttc tttatatgcc atatcctcac tgatcagcaa atgatcgcag
      181 gttgcctttg caaaaaattt ctccattaca gcaagcacaa aatccttcca gaattcagcc
      241 atttcttcaa caaattccgg atcttcaacc atcagcatac acaatccttc aaaaccgcac
      301 caatccctca attgccagaa tggacctgca aaacccagtg attgaagcca ggttctattt
      361 ttgtttgcca aggcaagttc atcaaaattt tcaggaaacc tcatcgggtc atcagggtca
      421 tatctttttt tcatctccag ccaatctgtt cttgtctcaa caggaaactt gtaccacttt
      481 cttgtaacaa agtcctttgc agcgcgcaga taagataaat cgtacttatc agaaatttca
      541 acaattgcac ccatccagtc ctgaacaatg taatggccat ctttatgctg aaggattttt
      601 tcttcaaaca tcggattcat cctgaatgaa acactgatgt attttgaaga accctgtttt
      661 tcgtatggaa tccctatgat ggaacataaa acttccatat aatccgtgtt ttcaggaagt
      721 ccttcagagt gccatcgttt gaatgttgat tcccttggtc cacctggaat aaagcagacc
      781 ttatccggtt ttgaaaaatc aagagatgca ataaatcttt ctctgaaatt cattgtgctc
      841 ctttctgttt ataaaatttt ttgttctgta cattatacca gatttcaatc gcctgttctg
      901 gctcttctcc aggtagaagt tgtggaacaa acatcaggtt actttttctt tcaagtggct
      961 cgagcaccct ttcgaaataa tcaggtaatg cttctccatc aaatcttttt acagtgccat
     1021 aataggtctt accatatttt acgaataatt ttattgtttc attccagtca aaacgctcag
     1081 ggttaccgaa attaataatt ttcacgtaat cacaatttaa aaaatactga agaagatgag
     1141 caccacttcc acaaaaatga acaaatccat taaattgttc aagggaccta ttgaaatatg
     1201 gatatactga cttgtaaatt tcaggagaaa gaagggtcgt tgtatcttca caacttctta
     1261 ccccacagca
//
LOCUS       DAORYU010000352         1266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_73585_length_1266_cov_4.459125, whole genome shotgun sequence.
ACCESSION   DAORYU010000352 DAORYU010000000
VERSION     DAORYU010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73585_length_1266_cov_4.459125"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..381)
                     /locus_tag="PLW07_07970"
     CDS             complement(118..381)
                     /locus_tag="PLW07_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50508.1"
                     /translation="MLKGWVNDENIHSDSRNIGIFFYQQLFASPQWYEVISCPVIATL
                     DAGNLETLTQSGFVIKSGSLKPVQEKNLYGCFRCTRCRAIEAN"
     gene            complement(382..>1266)
                     /locus_tag="PLW07_07975"
     CDS             complement(382..>1266)
                     /locus_tag="PLW07_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50509.1"
                     /translation="WQTESMKSPAVNLIKISPDGNRVITDFGSDGKIGPFTFHTSQVI
                     VSEKEGKIYVAGSHDSGHGTGVFSLKTGAPFYSIGGYFGTGNPKTTFTPSGIALGPDT
                     KIYIRFSAYDRTKEKDKGFLYTENPIAKRHSGYPPLIDNYWGPSMESSVPPDCFYASA
                     YQSDIAKIKDTGTGFVELYYLSVPGNPVGMSFDRGTGLLYAALRTTTGEIAVIHDTGI
                     SLNELWRLRDKNLGPTHAVKIKGNNLYVIEDGTKPVGRILTGMEKVKIEPQGKNKITK
                     YTLSFTEEKDVCRISVKK"
BASE COUNT          356 a          263 c          229 g          418 t
ORIGIN      
        1 aaatggctcc tgtaagcggg attaattcag gccttatttt cagaatgctg gacgatgtcg
       61 ttagtgaaaa acttgaaaaa ttaaaaagcc agaagacaga accagaaacc aaaccagtca
      121 gtttgcttca attgccctgc accgggtaca tctaaagcat ccgtataaat ttttttcctg
      181 cactggtttc aaactacctg atttaatgac aaatcctgac tgtgtcagtg tttccagatt
      241 gcctgcatca agggttgcga tgacagggca cgaaattact tcgtaccatt gtggacttgc
      301 gaataactgc tgataaaaaa agatgccaat attcctgctg tcagaatgaa tgttttcatc
      361 gttaacccat cctttcagca cttatttttt tacagaaatt ctgcagacat ctttttcttc
      421 tgtaaaactc agtgtgtatt ttgtaatttt atttttcccc tgtggttcta tttttacttt
      481 ttccatgcca gtaagtattc ttcccactgg ctttgtacca tcttcaataa catacagatt
      541 gtttcccttg atttttactg catgcgtggg accaagattt ttatccctga gtcgccacaa
      601 ttcattcaat gaaatcccgg tatcatgtat gactgcgatt tctccagttg ttgttcttaa
      661 tgcagcataa agaagtcctg tacctctatc aaatgacatg cctacaggat tccctggaac
      721 gcttagataa tataattcca caaaaccagt gcctgtatcc tttatttttg ctatgtctga
      781 ttgataggcg cttgcataaa aacaatcagg aggaacactt gattccattg atggacccca
      841 gtagttgtca atcaaaggcg gataacctga gtgtcttttg gctatcgggt tttctgtgta
      901 aagaaatccc ttatcttttt cctttgttct atcatatgca gaaaaacgaa tatagatttt
      961 tgtatcagga ccaagggcaa tgccagatgg agtgaatgtt gtttttggat ttccagtacc
     1021 aaaatagcca ccaatggaat aaaaaggtgc tcctgttttg agagaaaaaa cccctgtacc
     1081 atgcccacta tcatgtgaac ctgcaacata aatttttcct tctttctctg acacaataac
     1141 ctggcttgta tgaaatgtga atggcccgat ttttccatct gagccaaaat cagtaatgac
     1201 cctgttgcca tcaggtgaaa tttttataag attgactgct ggagatttca ttgattctgt
     1261 ttgcca
//
LOCUS       DAORYU010000353         1264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_73774_length_1264_cov_4.330025, whole genome shotgun sequence.
ACCESSION   DAORYU010000353 DAORYU010000000
VERSION     DAORYU010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73774_length_1264_cov_4.330025"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            209..725
                     /locus_tag="PLW07_07980"
                     /pseudo
     CDS             209..725
                     /locus_tag="PLW07_07980"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
     gene            complement(731..>1264)
                     /locus_tag="PLW07_07985"
     CDS             complement(731..>1264)
                     /locus_tag="PLW07_07985"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOL50510.1"
                     /translation="KVDKKADAILITHSHYDHCSIEDINKIVTDTTVVIGSPDSLAQV
                     KKGNKKTLSPGGEIDLGWVKINGVPAYNINKNFHPKSNNWLGFVIKFKDGSIYIAGDT
                     DFIPEMKNIKVDIAIVPVGGTYTMNAEEAAKAIDTIKPKIAIPIHFGDIVGSKADAEK
                     FASLVKSAQVKILTPGQ"
BASE COUNT          406 a          225 c          244 g          389 t
ORIGIN      
        1 agcaatagaa aaaaaactca ccacaaaagt tgggctcgaa atcactgtga agcttgaaga
       61 tggtagaaca gttgcaatca ttcaggaaaa aacacctgag gataacttca gaccaggaga
      121 tgctgttcag gtgatatatt catctgatgg tactgccaga gtaagaccaa agtaagttgg
      181 catctactgt caaacgaggt atatcctgtt ggaagtaaaa aatttcttaa tcacaggact
      241 tcctggttgt ggaaagacca cgctgattaa ggaattatcg tccgttattc caatcaataa
      301 gaaagggttc ttcacagaag aaatcagaga aaaaggtaaa agggcagggt tcaaaataat
      361 tacttttgat ggcaaacagg gcattttcgc ccatcaaatt tttatttctc catacagggt
      421 atcaaaatat tcagttctca gtgcttgaag agattggagt aagagaaatt gaagaatctc
      481 ttaatcagcc atgcctgata ttgattgatg aaattggaaa aatggaattg ttttcagaaa
      541 aattcaaaga agttgtgatc gctgcatttg agagcaaaaa cagggtactt ggcacagtat
      601 tgtattcctc acatccattt tgcgatgaaa tcaaaaaaag aaaagacacc cgcgctttta
      661 tccttgaaaa acataattac catcaattaa aacaaaatct actccattta cttcaacaat
      721 cctgaatttt ttattgtcca ggggttaaga ttttcacctg tgctgatttt accagggatg
      781 caaatttttc tgcgtctgct tttgagccaa caatatctcc aaaatgtatt gggattgcaa
      841 tttttggttt gatggtatca atggcttttg ctgcctcttc cgcattcatc gtgtaagttc
      901 caccaacagg tacaatggcg atatcaacct tgatgttttt catttcagga ataaagtccg
      961 tatcccctgc aatatagata gagccatcct tgaatttgat tacaaatccg agccagttgt
     1021 ttgatttagg atgaaagttt ttgttgatat tgtaagcagg aactccatta attttcaccc
     1081 agccaaggtc aatttcaccg cctggtgaaa gagttttttt attgcctttt tttacctgcg
     1141 caagactgtc aggagaacca ataacaactg ttgtatcagt aacaatttta ttgatgtctt
     1201 caatgctgca atggtcataa tgagaatgtg tgataaggat tgcatctgct tttttgtcaa
     1261 cctt
//
LOCUS       DAORYU010000354         1249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_75054_length_1249_cov_2.088777, whole genome shotgun sequence.
ACCESSION   DAORYU010000354 DAORYU010000000
VERSION     DAORYU010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75054_length_1249_cov_2.088777"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PLW07_07990"
     CDS             complement(<1..258)
                     /locus_tag="PLW07_07990"
                     /inference="COORDINATES: protein motif:HMM:NF014148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50511.1"
                     /translation="MRYKIAMIGGGSPGFSYFIAEALVNSEIFSDALFMLMDIDKKRL
                     NASVQRIKNLVKEKKSGLQVVSTTNRKIALEGADFVITSCEI"
     gene            complement(300..>1249)
                     /locus_tag="PLW07_07995"
     CDS             complement(300..>1249)
                     /locus_tag="PLW07_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50512.1"
                     /translation="EIKKQNVVLENFFYLIAAYKDKTGSEFSLVPATGGDLESGFVFN
                     KTDYHWVNQTEGILSKVELKCGLIGFTFKRANKLPTLLEPSFAGTRAIWLSRVERILA
                     SGAEGVDIRILCHHNNCVEWLTYAFAESVREYFKKMYGREPKADEKDYEKIRKIRGGF
                     FTDFLRDTRKMANKFNKKISVHFEPGIEVEPEVDTKMQIHWDWQTWLKERLMDEIHLK
                     YWSSQNPWIHCNVLPMARKMGIPIYIEDQATDPRADIRAIEMIENIVKDARVAGFDGY
                     IFYETWTYMILNRRGIPVPRGNAEGIIKTAYQIIQGSLK"
BASE COUNT          340 a          283 c          178 g          448 t
ORIGIN      
        1 aatttcgcag gaagttatta caaaatctgc tccttccagt gctatttttc ggtttgtggt
       61 agaaacaact tgaagccctg attttttttc ttttaccaga tttttaattc tttgaacact
      121 agcgttcaat ctttttttat caatatccat gagcataaaa agtgcatcag agaaaatctc
      181 tgaattgaca agtgcttctg ctataaaata ggaaaacccg ggtgagccac caccaatcat
      241 tgcaatcttg tatctcatac tatctttcct tgatattaaa caccatgcca tccaattgtt
      301 tattttaacg atccctggat tatctgatac gctgttttta ttattccttc agcgttacct
      361 ctcggcactg gaattcctct tctgttcagt atcatatatg tccatgtctc ataaaaaata
      421 tatccatcaa atccagcaac tcttgcatcc tttacgatat tttctatcat ctctatggct
      481 ctgatgtcag ctctgggatc agttgcctgg tcttcaatat atataggaat ccccattttt
      541 cttgccatcg gtaacacatt gcagtgaatc catggatttt gagatgacca gtatttcaga
      601 tgtatttcat ccatcaacct ttctttcaac catgtttgcc agtcccaatg tatctgcatt
      661 ttggtgtcaa cttctggttc cacttcaata cccggttcaa aatgtacgga aattttctta
      721 ttaaatttgt ttgccatttt tcttgtgtct cttaaaaagt cggtaaaaaa tcctccacga
      781 atttttctga ttttctcata atccttttcg tctgcctttg gttctctacc atacattttt
      841 ttgaagtact ctctcacgga ttccgcaaat gcataggtca gccattcgac acaattatta
      901 tggtgacaca aaattcttat atctacccct tctgccccac ttgcaagtat tctttcaacc
      961 cttgaaagcc agatagccct agtcccagca aaagatggtt caagaagggt tggtaactta
     1021 tttgctcttt tgaatgtgaa cccgattaaa ccgcacttca attcaacttt gctcaaaatc
     1081 ccctctgttt ggtttaccca gtggtaatct gtcttattaa aaacaaaccc actttctaag
     1141 tctccacctg ttgcaggaac aagagaaaac tcactacctg ttttatcctt atatgctgca
     1201 ataaggtaaa aaaaattctc aagtacaaca ttttgttttt tgatttcaa
//
LOCUS       DAORYU010000355         1238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_75963_length_1238_cov_2.889265, whole genome shotgun sequence.
ACCESSION   DAORYU010000355 DAORYU010000000
VERSION     DAORYU010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75963_length_1238_cov_2.889265"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="PLW07_08000"
     CDS             complement(<1..564)
                     /locus_tag="PLW07_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50513.1"
                     /translation="MKMQVAVVMIFSFFVITGFSQQEKTYTINEVLDKIGARIQQLPD
                     GQTIKNISGSMFLSIKLNDDDYNNLKSAAEKITGKTPSNPLKIDGNFVSVITVPGGKI
                     EKFFFAGKSEVCNFYMYRNYDTVVFLLPELGIEMEDRMSEIRKLTGKDKPGKGIMEDL
                     SAMMISSTLKTLFNQGKFWFYDAEMSVI"
     gene            complement(599..>1238)
                     /locus_tag="PLW07_08005"
     CDS             complement(599..>1238)
                     /locus_tag="PLW07_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50514.1"
                     /translation="VNLPEKSQLWSVYVANNPTKPLAGKSANNVLIPLLRTSTKTDSA
                     LPVEVIYYQSGIPFGKTGEFQINLPGVDIPVMHLMYSLYIPEKLNLDNFNGNIEKVEK
                     FSSVESESAAKILEMEAAKGEIKKPIARKDISQSVVYVENQEILERNVQAAISGRISG
                     LSNRALSMDESGAGLLPLKIYIPTTGKLLRFEKRLVMDENLFLKARYKLVSR"
BASE COUNT          335 a          247 c          223 g          433 t
ORIGIN      
        1 aatcactgac atttcagcat cataaaacca gaattttcct tgattgaata atgttttcag
       61 tgtgctggaa atcatcatag cactcaggtc ttccattatt cccttgcctg gtttatcttt
      121 ccctgtaagt tttcttattt cagacattct gtcttccatt tcaatcccca gttctggaag
      181 cagaaatact acagtatcat aatttctgta catgtagaag ttgcacactt cagattttcc
      241 agcaaagaaa aatttttcaa tttttcctcc tggaactgtg atgacagaaa caaaatttcc
      301 atcaattttc agtggatttg atggtgtttt tccagtgatt ttttcagccg cagatttgag
      361 attattgtaa tcgtcatcat tgagtttgat tgaaagaaac attgaacctg aaatattttt
      421 gatggtttgt ccatcaggta gttgctggat tcttgcacca attttgtcaa ggacttcatt
      481 gattgtatat gttttttctt gctgggaaaa ccccgtaata acaaaaaaac tgaaaatcat
      541 tacaactgcc acttgcattt tcatcacgga tttcctcctg tttatctgca aaatgccatt
      601 atcgcgacac aagtttgtat cgtgccttga ggaataaatt ctcatccatc acgagccgtt
      661 tttcaaacct caatagtttc cctgttgtcg gtatgtatat tttcaatgga agaagcccgg
      721 cgccactttc atccattgat agtgcacgat tcgaaaggcc tgatattcta ccagatatcg
      781 ctgcttgtac gtttctttca agaatttctt gattctcaac gtagaccacg gattgcgaga
      841 tgtccttacg ggcaatgggc ttttttattt ctcctttggc tgcttccatt tcgagaatct
      901 ttgccgcact ttcactctca actgaggaaa atttctcaac cttttcaata ttgccgttga
      961 agttatcgag gttaagtttt tctggaatgt aaagagaata catcagatgc ataacaggaa
     1021 tgtccacgcc aggaagattg atttgaaatt caccagtttt tccaaacggt attcctgatt
     1081 gataataaat gacctcaacc ggtaaagcag aatctgtttt tgtggatgtt ctgagaagtg
     1141 gtatcaatac attattggct gatttgccag caaggggttt tgttggattg ttcgcgacat
     1201 atacgctcca caactgactt ttttcaggaa gattgact
//
LOCUS       DAORYU010000356         1235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_76218_length_1235_cov_4.427119, whole genome shotgun sequence.
ACCESSION   DAORYU010000356 DAORYU010000000
VERSION     DAORYU010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76218_length_1235_cov_4.427119"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="PLW07_08010"
     CDS             complement(<1..327)
                     /locus_tag="PLW07_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOL50515.1"
                     /translation="MFVPAYKKLPEATFDEKIRQLDKILSECVLCPRKCGVNRKKGEK
                     GYCRADANLCISSYGAHSGEENILVGKFGSGTIFMSWCNLRCCFCQNYDISLKGVGQI
                     VSIEQCV"
     gene            complement(361..>1235)
                     /locus_tag="PLW07_08015"
     CDS             complement(361..>1235)
                     /locus_tag="PLW07_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50516.1"
                     /translation="CYSNMFHLPEKNLPVSSNTYTIFCPYLRQYKHKFFEPGFNQKDV
                     TLGAKYPDEEPINPVDDREYGILLSQWLPYLKKIGSVLGIFSYYQLVFHDQTERSDRS
                     RYLYQPEPALVEDEINWFIKSGMKVFYDCSPPYPGFWPDGRFYAYLPRLLWENSPNAD
                     RFVMEYYKAIAGERAEVLKNILKTISGFINNNESDEIPSSDIVNIAKGIFNSLNSPLK
                     QKYKLWFDYVLLGKQSWKALKQKNIGDMIEAEKKIIAFFQDNRSVLEDFINVDYMIKH
                     SQAIIDFYTSQENL"
BASE COUNT          375 a          225 c          213 g          422 t
ORIGIN      
        1 tacgcactgc tcaattgaaa caatttgacc cactccttta agggaaatgt catagttctg
       61 acaaaagcaa catctgagat tgcaccagga cataaaaatt gttccagaac caaatttgcc
      121 aacaagtata ttttcttctc cagaatgggc gccatagctt gatatacaaa gatttgcatc
      181 agcccggcaa taaccttttt cgcctttctt tctgttaaca ccgcattttc tgggacacag
      241 gacgcattct gaaagaattt tatcaagttg ccttattttt tcatcaaacg ttgcttctgg
      301 caatttttta taagcaggaa caaacataat tccctgaaat atttagaatg ttcatgttta
      361 tcagaggttt tcttgagaag tataaaaatc tataattgcc tggctatgtt ttatcatata
      421 gtcaacattg atgaaatctt caagaactga acgattgtcc tggaaaaaag caataatctt
      481 tttttctgct tcaatcatgt caccaatatt cttttgtttc aatgctttcc aggattgttt
      541 tccaagcaag acataatcaa accatagctt atacttttgt tttaacggtg agtttaatga
      601 attgaaaatt cccttcgcaa tgttaacaat atctgatgaa ggaatttcat cgctttcatt
      661 attgttgata aaacctgata ttgttttcag gatatttttc aatacttcag ctctttctcc
      721 tgcaattgct ttgtaatatt ccattacaaa tctgtcagca tttggagaat tttcccatag
      781 cagtcgtggt agatatgcgt aaaatcttcc gtctggccag aaaccagggt atggtggaga
      841 acagtcgtaa aatactttca ttcccgattt tataaaccag ttaatttcgt cttcaacaag
      901 ggcaggttct ggttgatata aataacgtga cctatcagat ctttctgtct ggtcatgaaa
      961 tactaactgg taataggaga atatgccgag aaccgaacct atttttttaa gatagggtag
     1021 ccattgcgac agtaaaattc catattctct gtcatccaca ggatttattg gttcttcatc
     1081 aggatatttt gcaccgagtg ttacatcctt ttgattgaat ccgggttcaa aaaatttatg
     1141 tttatattgc ctgagatacg ggcagaaaat tgtataagtg tttgaagata cagggagatt
     1201 tttctctgga agatggaaca tgttcgaata acaga
//
LOCUS       DAORYU010000357         1231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_76554_length_1231_cov_5.527211, whole genome shotgun sequence.
ACCESSION   DAORYU010000357 DAORYU010000000
VERSION     DAORYU010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76554_length_1231_cov_5.527211"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="PLW07_08020"
     CDS             <1..788
                     /locus_tag="PLW07_08020"
                     /inference="COORDINATES: protein motif:HMM:NF012796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HOL50517.1"
                     /translation="VRSWKNLPVMLYQIQTKYRDEIRPRFGIIRSREFLMKDCYSFDR
                     TQQESEQSYLKMRQAYLKIFNRCSLNVTVQSADSGVIGGKFSEEFVAAGDCAELEIGH
                     IFKLGTDYSEKLHVVYMDKDGTEKPVVMGCYGIGVSRIVAAIIEGSHDDRGIIWPVSV
                     APFHATVVPVNMSDSRAVETANKIHDEFESSEIEVLFDNREESAGVKFADADLIGIPY
                     RITLGRRLVEGKVEVMERKTRKTYDVEAGKVKDFMLSAMKQST"
     gene            785..>1231
                     /locus_tag="PLW07_08025"
     CDS             785..>1231
                     /locus_tag="PLW07_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50518.1"
                     /translation="MNMLTGILKSGFSFNRDRIKLFLVVFCSTLFLMQAIEYIFKKDA
                     QNQLLWLMIIFLVENFLTCGVYGSLKNVLDGTPLTVRTFLMNCLRFFTRFLLIKLLFV
                     IFIALITGLLMLVAQATGRLSIPAAAGIVLLWLVWLAFPSYYFVLSL"
BASE COUNT          355 a          214 c          278 g          384 t
ORIGIN      
        1 atgtaagaag ctggaaaaat cttcctgtga tgctctatca gatacaaacg aaatatcgtg
       61 atgaaatcag gccaagattt ggaatcatcc gttccaggga atttcttatg aaggactgct
      121 acagttttga cagaacccaa caggagtctg aacagtcgta tcttaaaatg agacaggcat
      181 atttgaaaat tttcaataga tgttcgctca atgtaacagt gcaatcagca gattctggtg
      241 tcattggcgg gaaattctct gaagaatttg ttgctgcggg agattgtgca gaacttgaaa
      301 taggtcatat ctttaagctt ggcactgatt atagcgaaaa attacacgtg gtctatatgg
      361 ataaagatgg cactgaaaaa cctgtggtga tgggatgtta tggcatagga gtgagcagga
      421 ttgtcgctgc gatcattgag ggaagtcatg atgacagagg cattatctgg cctgtttcag
      481 tggcgccttt tcatgccact gttgttcctg taaacatgtc agatagccgg gctgttgaaa
      541 ctgcaaacaa aattcatgat gaatttgaat cttcagaaat agaggtgctt tttgacaaca
      601 gggaagaatc cgcaggcgtt aaatttgcag atgcagacct tattggcata ccttacagaa
      661 ttacactcgg tagaagactt gttgaaggaa aggttgaggt tatggaaaga aaaaccagga
      721 aaacgtatga cgttgaagct ggaaaagtga aggactttat gctgtctgca atgaaacaat
      781 caacatgaat atgcttacgg gtatccttaa atcgggtttc tcattcaaca gggacagaat
      841 taaactcttt ctcgttgtat tctgttcaac tcttttcttg atgcaagcta ttgagtacat
      901 attcaaaaaa gacgcacaaa accagttact ctggctcatg ataatctttc ttgtggaaaa
      961 tttcctgaca tgcggagttt atggttctct caaaaatgtt ctggatggga cacccctgac
     1021 agtaagaacg tttttaatga actgtttgag gtttttcacc cgttttcttt tgataaaact
     1081 attgtttgtg atttttatcg ctttaatcac aggattactt atgcttgttg ctcaagccac
     1141 aggtagactt tctattcctg cggctgcagg cattgtgtta ttgtggctgg tatggcttgc
     1201 ttttccatcc tattatttcg ttctctcgct t
//
LOCUS       DAORYU010000358         1205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_78955_length_1205_cov_4.347826, whole genome shotgun sequence.
ACCESSION   DAORYU010000358 DAORYU010000000
VERSION     DAORYU010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78955_length_1205_cov_4.347826"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /locus_tag="PLW07_08030"
     CDS             <1..1006
                     /locus_tag="PLW07_08030"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF022449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOL50519.1"
                     /translation="DDAYNVFDLFRDIPCNQEFEIADGYTLICHNAGHILGSAIFEFI
                     EKKTGKNLVFTGDLGRPNRPLLPEPERLEKIDYLVMESTYGDRVHESDEIAYETVART
                     ITETAEKSGNIVVPSFAIERTQELLYCLKKLLAENRIPHLITFIDSPMAIKVTEVFKR
                     YPEFLRKTINDMVNNDDSPFEMELLHPTETVDQSKSINHVRGSVIIIAGSGMCTGGRI
                     KHHLVTNISRPESAIMFVGYQATGTLGREIISGKKQVRILGEYRQIRANIVQVNGFSS
                     HADQNEILWWLERLEKSPINTFLVHGEEPSMIKLKDVLMERKSIKANIAEYQTSVRLE
                     "
     gene            1003..>1205
                     /locus_tag="PLW07_08035"
     CDS             1003..>1205
                     /locus_tag="PLW07_08035"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOL50520.1"
                     /translation="MKILLTGCAGFIGWHTAKTALNSGYEVFGIDDINDYYDVRLKQW
                     RLDDLKKMKNFTFQQVDIREQQKL"
BASE COUNT          398 a          200 c          269 g          338 t
ORIGIN      
        1 ggatgatgca tataatgttt ttgatctttt tcgtgacatt ccgtgcaatc aggagtttga
       61 aatagcagat gggtacacac taatctgcca taatgcaggt catatacttg gttctgcgat
      121 atttgagttc atagagaaaa aaacaggcaa aaaccttgtt tttacaggag accttggaag
      181 acccaataga ccacttcttc cagaaccaga gcgacttgaa aaaattgatt atcttgtgat
      241 ggagtccaca tatggagaca gggtccacga gtccgatgag attgcctacg aaacagttgc
      301 tcgtaccatt actgaaactg cagaaaaatc cggcaatatt gtggtgccga gttttgccat
      361 tgaaagaacc caggaacttt tgtattgtct gaaaaaattg cttgctgaaa atcgaattcc
      421 gcatctgatt acttttattg atagtccgat ggcaataaag gtgacagaag tatttaaaag
      481 atatcctgaa tttttacgaa aaaccatcaa tgacatggta aacaatgatg atagtccttt
      541 tgaaatggaa ttgcttcatc ccactgaaac agttgatcaa tcaaaatcga ttaatcatgt
      601 cagggggtct gtgattatca ttgcaggttc aggtatgtgc actggtggta gaataaaaca
      661 tcaccttgta accaatatca gtaggcctga aagcgccatc atgtttgttg gatatcaggc
      721 aacaggaacc ctcggcaggg aaatcataag cggcaaaaag caggtaagaa ttcttggtga
      781 atataggcag ataagggcaa acattgtgca ggtgaatggt ttttcttctc atgctgatca
      841 gaatgagata ctgtggtggc tggagcgtct tgaaaagagc ccgattaata cctttcttgt
      901 tcatggtgag gaaccttcaa tgataaaatt gaaagatgtt ctcatggaga gaaaatccat
      961 caaagcaaat attgcagaat atcaaacatc agtgaggtta gaatgaagat tcttttaaca
     1021 ggatgtgcag gttttattgg ctggcataca gcaaagacag cgctgaactc aggatatgaa
     1081 gtttttggta tagatgatat caatgactat tatgatgtga gattgaaaca gtggcgtctt
     1141 gatgacctga aaaaaatgaa aaattttacc tttcaacagg ttgatatcag ggagcaacaa
     1201 aaact
//
LOCUS       DAORYU010000359         1203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_79137_length_1203_cov_3.577526, whole genome shotgun sequence.
ACCESSION   DAORYU010000359 DAORYU010000000
VERSION     DAORYU010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79137_length_1203_cov_3.577526"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..955
                     /locus_tag="PLW07_08040"
     CDS             113..955
                     /locus_tag="PLW07_08040"
                     /inference="COORDINATES: protein motif:HMM:NF038287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Amuc_1100 family pilus-like protein"
                     /protein_id="HOL50521.1"
                     /translation="MKKILSFLKKNLNFVFLIIVLGMLGFGYNLIKKTKQELKKMEEE
                     IKAKHEEIRKYQTQKELAPSPELVSKLQQQYVTVAGQFNSMLNRFSTTYPTVPEFKVF
                     PNVEFKEFLFETGDSLKKKARKNDVIIPSSFGFQETGFPSSDQIPLFSLQITIIKHIL
                     DLLIDSGVSVVNSVVPGIPSTVAFYKILPLDVSITGTSIEIVKFMRYLNNPSSFFTLE
                     SFAVTKRGSGMFQANFKINAVMIEKAATVPETITVAKAQTPGSSGGNVPSPQPAGVPP
                     GTAK"
     gene            964..>1203
                     /locus_tag="PLW07_08045"
     CDS             964..>1203
                     /locus_tag="PLW07_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50522.1"
                     /translation="MKKDFIRKWRSLDRNGRRNLVRQFIAAKQTSSELPVEKKHDSVL
                     NDSTIKKHTFRKITSFMKLKPIFAFGFASSGSSGES"
BASE COUNT          381 a          221 c          242 g          359 t
ORIGIN      
        1 gttttgttca gaagctttca aaattggaat ttttctctga ggtgaacatt gactcctgtg
       61 aacttgaccg tgaaacagga aaactgaaat ttcttttgac tgccaaagtg aaatgaaaaa
      121 aatactgagt tttttgaaga agaatttgaa ttttgtgttt ctcattattg ttcttggaat
      181 gctgggattt ggttataatc tgataaaaaa gaccaagcag gaactgaaaa aaatggaaga
      241 agaaattaag gcaaaacacg aggaaataag aaagtatcaa acccagaaag aattggcgcc
      301 atcgccagaa ctcgtcagta aattacaaca gcaatatgtg acggttgcag ggcagttcaa
      361 ttcaatgctg aatcgtttct ccacgaccta cccgacagtg ccggaattta aggtttttcc
      421 caatgttgaa ttcaaggaat ttttatttga aacaggagat tctttaaaaa agaaagcccg
      481 caagaatgat gttattattc catcctcctt tggttttcag gaaacgggtt ttccctcatc
      541 tgaccagatt cctttgtttt cgcttcagat tacaattatt aaacatattc ttgacctttt
      601 gattgattca ggggtaagcg tggttaattc agtagtacca ggcattccgt caactgttgc
      661 cttttataaa atacttcccc ttgatgtctc catcactggc accagcattg agatagtcaa
      721 gtttatgcgt tatctcaaca atccttcgtc attttttacc cttgaaagtt ttgcagtgac
      781 caagagaggt tcagggatgt ttcaggcaaa ttttaagata aacgctgtga tgatagagaa
      841 ggctgcaact gttccggaaa ccatcactgt tgcaaaagcc caaacgccgg gttcctcagg
      901 tggaaatgtt ccatcaccgc aacccgcagg agtaccacct ggtacggcta aataggacca
      961 ctgatgaaaa aagattttat caggaagtgg agaagtcttg atagaaacgg aagaagaaat
     1021 cttgttcggc aatttattgc agcaaagcag accagttcag aacttcctgt tgaaaaaaag
     1081 catgacagtg ttcttaatga tagtactatt aaaaaacata cattcagaaa aattacttct
     1141 ttcatgaaat tgaagccgat ttttgctttt ggtttcgctt cttctggttc atccggagaa
     1201 tca
//
LOCUS       DAORYU010000360         1200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_79430_length_1200_cov_4.248908, whole genome shotgun sequence.
ACCESSION   DAORYU010000360 DAORYU010000000
VERSION     DAORYU010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79430_length_1200_cov_4.248908"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..632)
                     /gene="rdgB"
                     /locus_tag="PLW07_08050"
     CDS             complement(42..632)
                     /gene="rdgB"
                     /locus_tag="PLW07_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOL50523.1"
                     /translation="MSFKIFLATKNPAKLREIKEILKKFDCTVLLPDVADFPPETGQT
                     FQENALIKASFVGRMYPQHYVAGEDSGLVVPALNGMPGVLSARFAGPDANDGKNIEKL
                     LQYSKNLKDGEREAYFICVIALIEPSGNHRFFEGRLYGRIVQEPSGSNGFGYDPIFEI
                     PEYGKTVAQLSVEEKNKISHRAKAFLQLAEYLEMAV"
     gene            complement(604..>1200)
                     /gene="rph"
                     /locus_tag="PLW07_08055"
     CDS             complement(604..>1200)
                     /gene="rph"
                     /locus_tag="PLW07_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HOL50524.1"
                     /translation="KVPPFLQGTGSGWVTAEYGMLPASTPERNSRTNNQSGRSQEIRR
                     MIGRVLRAAVNLKNIGERTLIVDCDVIQADGGTRTASINGAFCAIVDALVQMRKRNLL
                     MLPVLRECIGAVSVGLVNGNKYLDLNYSEDSIAQVDFNVVMNGKGEFVEIQGAAEGMF
                     FSRNELDQLIVIAQKGIKEIIEAEKNFLKNELQNLSGY"
BASE COUNT          334 a          309 c          197 g          360 t
ORIGIN      
        1 cccagacgta tcgggtatga gccgactgct ctgccactga gctacaccgc catttcaagg
       61 tactctgcta attgcaaaaa tgcctttgcc ctgtgactga ttttattttt ttcttcaaca
      121 gacaactggg caactgtttt gccgtactca ggaatctcaa atatcgggtc gtagccaaac
      181 ccgtttgaac ccgatggttc ctgaacaatc cttccatata atctaccttc aaaaaacctg
      241 tgatttccag agggttcaat caacgcaatc acacaaataa aatacgcttc cctctctcca
      301 tcttttagat ttttactata ttgtaacagt ttttcgatat tttttccatc gttcgcatct
      361 ggtccggcaa accgcgcaga caaaactcct ggcattccgt ttaatgcagg aacaacaaga
      421 cctgaatctt caccagcgac ataatgctgc ggatacatcc gtccgacaaa tgatgctttt
      481 atcaaagcat tttcctgaaa tgtttgtccg gtttcaggag gaaaatccgc aacatcaggc
      541 aaaagaactg tgcaatcaaa cttcttcaaa atttccttaa tttccctgag tttggcagga
      601 ttcttagtag ccagaaagat tttgaagctc atttttcaaa aaattttttt ctgcctcaat
      661 gatttcttta ataccctttt gtgcaatgac tatcaattgg tcaagttcgt tacggctgaa
      721 aaacattcct tctgcagcac cctgaatttc cacgaattcc cctttgccat tcataacgac
      781 attaaagtca acctgtgcaa tagagtcctc agaatagttc agatcaagat acttattacc
      841 attgacaaga ccaacactca ctgcaccaat gcattccctt aaaacaggca acatcaaaag
      901 gtttctcttc ctcatctgaa caagagcatc cacaattgca cagaaggcgc catttatcga
      961 tgctgtacga gtaccaccgt ccgcctgaat cacgtcacaa tcaactatca atgtcctttc
     1021 tccgatattt tttagattaa ccgctgccct taaaaccctt cctatcatgc gcctgatttc
     1081 ctgacttctt cctgactgat tgtttgttct cgaatttcgt tcaggtgtgg atgcaggcaa
     1141 catcccgtat tctgcggtaa cccatcctga acctgtgccc tgaagaaaag gtggaacttt
//
LOCUS       DAORYU010000361         1192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_80180_length_1192_cov_3.153034, whole genome shotgun sequence.
ACCESSION   DAORYU010000361 DAORYU010000000
VERSION     DAORYU010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80180_length_1192_cov_3.153034"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..1055)
                     /locus_tag="PLW07_08060"
     CDS             complement(99..1055)
                     /locus_tag="PLW07_08060"
                     /inference="COORDINATES: protein motif:HMM:NF017847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HOL50525.1"
                     /translation="MKIALLGYSGSGKTEIVAALSGKKHESFDPKKPAVISIKIYDRQ
                     LQTIAEKVKPEKTTASEITFIDIKGSPESSGLDETIIEHALFSDRIALVIPAFIEERN
                     PVNELDSLCLELIYRDIDRVNGILEKRKQEISHGKRKKSHEEELLEKCLSHLGKESLL
                     SSMEMRSEEKVFLDSLGLVTSKKFFVMANGNILITETQTRATQYHCGFCQIDARNVNK
                     ETIEIFWKQLLLAAGLIRFYTIVGKETRAWLIPEGSSVLDAAAAIHTDIAKGFVRADV
                     VTCEDFISLGSFAACREKGLLRSEAKTGLVKDGDIIHIHSTR"
BASE COUNT          318 a          256 c          212 g          406 t
ORIGIN      
        1 tgctggtctt tctccctcaa tggaacactg accacacata tctttgcacc tgaattgtct
       61 gcgcgtctta atatgagcgg aacatcattt tccatctttc acctcgtgga gtgtatgtga
      121 atgatatctc catcttttac aagtcctgtt tttgcttcac ttcttaatag gcctttttcc
      181 ctgcatgccg caaaagaacc aagcgaaata aaatcctcgc atgttactac atctgccctg
      241 acaaagcctt ttgcaatgtc tgtgtgtatg gctgctgctg catcaaggac actggatcct
      301 tctggtatca accatgccct tgtttcctta cccacaattg tataaaaccg tatcaaccct
      361 gctgcaagca gaagttgttt ccagaatatt tctatggtct ctttattgac attgcgggca
      421 tctatctggc agaaaccaca atgatactgt gtggctcgtg tttgtgtttc tgttatcaag
      481 atatttccat tggccatcac aaaaaacttt tttgatgtaa caagcccaag cgaatcaaga
      541 aaaacctttt cttctgaccg catctccatt gatgaaagta gggactcttt tccaagatgg
      601 cttaaacatt tttcaagtaa ttcttcttca tgactctttt tccgttttcc gtgtgaaatt
      661 tcctgtttcc ttttttctaa aattccattg accctgtcaa tatctctgta gataagttcc
      721 agacacaatg aatcaagttc gttcacaggg tttctttctt caatgaatgc aggaatgaca
      781 agcgctatcc tgtcggaaaa cagtgcgtgc tcaataattg tttcatcaag acctgaactt
      841 tctggagagc cctttatgtc aataaatgta atttctgatg ctgtcgtttt ttctggtttc
      901 actttttctg ctattgtctg aagttgtctg tcataaattt taatagaaat cactgctggc
      961 tttttcgggt caaaactttc gtgttttttg cctgaaagcg cagccacaat ttctgttttg
     1021 ccgctacctg aataaccaag caatgcgatt ttcatatttg actgtgtatt ttacatcaga
     1081 atttttgaaa atcaagcaag attgaagttg gtcagtgttt atgtacaata agaggaaagg
     1141 agaacaaaaa aactgcctgt acccaataaa ggaaaacgat gagatgacag gt
//
LOCUS       DAORYU010000362         1169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_82502_length_1169_cov_6.096948, whole genome shotgun sequence.
ACCESSION   DAORYU010000362 DAORYU010000000
VERSION     DAORYU010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82502_length_1169_cov_6.096948"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="PLW07_08065"
     CDS             <1..572
                     /locus_tag="PLW07_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50526.1"
                     /translation="TKSSVPIEKKFTFDARKLPWDPEKVMGNVGIPVTYEIKNDKNSG
                     LGEFVLWDGKARIFQDDGKRTTIFLGEDNVSFTPVGEKCSLYVGDSRDIVVTQRKIKE
                     TKINVRKNDSNAVILYDTDEIIKVDIENFKDMPATLTLIQEIPGEWDMEETNHSYEKE
                     DANTLKFNIQLAPKAKQTVTFHYHRRNVR"
     gene            569..>1169
                     /locus_tag="PLW07_08070"
     CDS             569..>1169
                     /locus_tag="PLW07_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50527.1"
                     /translation="MKKTIIFSLVFLFSSVLYCKIDLVTLPERDKVQLTIYNSADLTL
                     VRESRTLTMKKGTNKLQFSWANTLIDPTSLELFPLKDADKIAVTDLYFPPRITGLGIW
                     NIVSQISGKAPCEINYFTSGISWQAYYLATLSQDEKTMKLEGYVKVTNRSGEDYENAQ
                     VRLVVGKIHLLDEIATLARRYPPYGRPFEPKPLPPTEPGK"
BASE COUNT          427 a          223 c          230 g          289 t
ORIGIN      
        1 tcacaaaatc cagtgtgccg attgagaaaa aatttacgtt tgatgcaaga aaactaccat
       61 gggaccctga aaaagtcatg ggaaatgttg gaattccagt gacatacgag atcaaaaatg
      121 ataaaaactc aggccttggc gaatttgtat tgtgggatgg aaaagcaaga atttttcagg
      181 atgatggaaa aagaacaaca atttttcttg gcgaagataa tgtcagtttc actccagtag
      241 gcgaaaaatg ttcattgtat gtcggggata gcagggatat tgtcgtcacg cagagaaaaa
      301 ttaaggagac aaaaatcaat gtaagaaaaa atgacagtaa tgccgtaatt ctctatgata
      361 ctgatgaaat cataaaggtt gacattgaaa acttcaagga catgccagca acattgacac
      421 ttattcagga aatccctggt gaatgggata tggaagaaac aaaccattcg tatgaaaaag
      481 aagatgcaaa tacattgaaa ttcaatattc aacttgctcc aaaagcaaaa caaactgtca
      541 cattccatta tcacaggagg aacgtcagat gaaaaaaaca atcatttttt ctctggtatt
      601 tctgttcagt tcggttctat actgcaaaat tgaccttgtc acacttccag aaagagataa
      661 agtgcaacta acaatttaca attctgctga tttgacactg gtaagagaaa gcagaacatt
      721 gacaatgaaa aaaggaacca ataaactgca gttttcatgg gcaaataccc tgattgaccc
      781 gacatccctt gaacttttcc cgctcaaaga tgcagacaaa attgctgtca cagaccttta
      841 ttttcctcca agaatcacag ggcttggcat ctggaacatt gtctcacaga tttcaggaaa
      901 agcgccatgc gaaatcaatt attttacttc aggaatttca tggcaggcat attatcttgc
      961 aacactctct caggatgaaa aaacaatgaa actcgaagga tatgtcaagg tgacaaaccg
     1021 cagtggagaa gattatgaaa atgcgcaggt gcgacttgtt gttggcaaaa tccacctcct
     1081 tgatgaaatc gcaacccttg caagaagata tccaccctat ggaagaccct ttgagccaaa
     1141 accattgcca ccaacagaac caggaaagc
//
LOCUS       DAORYU010000363         1150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_84484_length_1150_cov_5.770776, whole genome shotgun sequence.
ACCESSION   DAORYU010000363 DAORYU010000000
VERSION     DAORYU010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84484_length_1150_cov_5.770776"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1000)
                     /locus_tag="PLW07_08075"
     CDS             complement(<1..1000)
                     /locus_tag="PLW07_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50528.1"
                     /translation="MSNFVATSLYNPEIVNYIISLIQGDTVKKKIFLFGILSLLVSAF
                     SATGQQQSQTAPFQYVYAKAYHVLPGTHNNESGYFSLCEGLDGKIYIGTAKYNENSYL
                     VEFDPYTESQKIVIDTHRVCGLNATGYAAQAKIHTKNFVGSSGKIYVGSKQGYRSPGD
                     TSEYPGGYVMAYDPRTQKAESLGMPYPGEGVIDVVADEQRHLIYVVTCENQHWMLYDE
                     GTKKYRELGVLLLPYATTIIDKNGKAHAITNDFKIATYDPAKDTLSIRPIIVGRKILT
                     KPHGKGYAICFWVLAGDGKTAYMTMLSWPDLYAIDLGSKGKTVKAQYLGKMIEGKNP"
BASE COUNT          320 a          238 c          221 g          371 t
ORIGIN      
        1 caggattttt gccttcaatc attttgccga ggtattgtgc tttgactgtt tttcctttac
       61 ttccaaggtc tattgcataa aggtcaggcc agctcagcat tgtcatatag gcggttttac
      121 catcaccagc aagcacccag aagcaaatcg cataaccttt gccatgaggt ttggtaagaa
      181 ttttccttcc tacaatgatg ggtctgatgg aaagggtgtc ttttgcaggg tcatatgttg
      241 cgattttaaa atcattggta attgcgtgcg cctttccatt tttatcaatg attgtcgttg
      301 catagggtaa cagaagcact ccaagttctc tgtacttttt tgttccttca tcgtacagca
      361 tccaatgctg gttttcacat gtgacaacat atatcagatg cctttgctcg tctgcaacga
      421 catctatcac tccttcacct ggatatggca ttccgagaga ttctgccttt tgagttctgg
      481 ggtcatatgc catcacatag ccaccaggat actcagaagt atccccagga ctgcggtatc
      541 cctgtttgct tcccacgtaa atttttccac tcgagcccac aaaatttttg gtatgaattt
      601 ttgcctgcgc agcataacca gttgcattaa gtccgcatac tctgtgtgtg tcgatgacaa
      661 tcttctgact ttctgtgtac gggtcaaatt ccacaagata ggaattttca ttgtactttg
      721 cagtaccaat gtatatcttt ccatcaagac cttcgcagag agaaaaataa cctgattcat
      781 tgttgtgagt gccaggaaga acatgatatg cctttgcata cacatactga aatggtgcag
      841 tttgagattg ctgctgacct gttgcagaaa atgcagaaac caataaagac aaaatcccaa
      901 aaagaaaaat tttctttttc actgtgtctc cctgtattag tgaaataata taattcacaa
      961 tttctggatt gtaaagagat gtcgccacaa aatttgacat acattttcat atgtgtataa
     1021 ataaacacaa ccaaaaattt ttaaaagggg gtgaaaaaaa tgaaaagaaa gggttttacg
     1081 cttattgaac ttctggtagt aatagccatt attgcagttc ttgctgcgat gttgcttcct
     1141 gcactcagta
//
LOCUS       DAORYU010000364         1146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_84959_length_1146_cov_4.185151, whole genome shotgun sequence.
ACCESSION   DAORYU010000364 DAORYU010000000
VERSION     DAORYU010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84959_length_1146_cov_4.185151"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..511)
                     /locus_tag="PLW07_08080"
     CDS             complement(152..511)
                     /locus_tag="PLW07_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50529.1"
                     /translation="MRYAIMIVLAATLMFGYAMTLCAQEKAAQPTATQDEMMAKKKQA
                     AEIMGKMRNIERQVASQDEECKKITVQMTELEKQRRARLETLLSGNEEYQKLKKQLQE
                     LMPPPPQKEAPKEAPKK"
     gene            complement(588..>1146)
                     /locus_tag="PLW07_08085"
     CDS             complement(588..>1146)
                     /locus_tag="PLW07_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOL50530.1"
                     /translation="QAFGTLMYQGKVVYAGSSNFAAWDIATACQEARKHNLTGLVSEQ
                     SIYNLLNRTIELEVIPACRYYGLGLIPWSPLAGGLLAGINGKIKEGRRISENVKKSAE
                     QKQDQLKKYQALCKKIGEEPATVALAWLLHNPAVTAPIIGPRTVEQLERAIRATEITL
                     DEEILQKLDEIFPGPGGEAPKAYAW"
BASE COUNT          270 a          269 c          214 g          393 t
ORIGIN      
        1 tttgaaatac caaaaatcag tgtaatcaca aatatttttc tcattttccc tccatcgttg
       61 tcttttagac atcataatac agaggtaagt ttattgcaaa agaaaagggc tggaatttac
      121 cagcccttcc tctccttttt gccagaagac gttacttttt cggtgcctct ttcggtgctt
      181 ccttttgagg tggaggaggc atcaattcct gaagttgttt tttcaatttc tgatattcct
      241 cattccctga tagtagtgtt tcaaggcgag ctctgcgctg tttttcaagt tcagtcatct
      301 ggacagtaat tttcttgcat tcctcatcct gggaagcaac ctgcctttca atatttctca
      361 ttttgcccat aatttctgct gcctgttttt tctttgccat catctcatcc tgggttgctg
      421 taggttgggc agccttttcc tgggcacaaa gcgtcatcgc atagccaaac atcaatgttg
      481 ctgcaagaac tatcatgatt gcatatctca tgatttcacc ccctttcgtt tatttgttaa
      541 taattagatt accatattct gtgaaggttt agtattgtgg atatccatta ccaggcatat
      601 gcctttggtg cttcacctcc tggaccagga aatatctcgt caagtttctg caatatctcc
      661 tcatcaagtg tgatttctgt tgccctgata gcgcgttcaa gttgctctac tgtgcggggt
      721 cctatgatgg gtgctgtaac agcaggattg tgcaacaacc aagcaagggc aacagttgca
      781 ggttcttcac ctattttttt gcacagtgcc tggtattttt tcaactggtc ctgtttttgt
      841 tcagcgcttt ttttaacatt ttcactaatt ctcctaccct ctttaatttt tccgtttata
      901 ccagccagaa ggccgccagc aagtggactc catggaatca aaccaagtcc ataataacga
      961 caggcaggaa ttacttcaag ttctattgtt ctattgagaa gattataaat gctctgttca
     1021 cttaccagcc ctgtaagatt gtgtttccgt gcctcttggc aggcagtcgc aatatcccag
     1081 gcagcaaaat tgcttgaccc agcatacaca accttgccct gatacatcaa ggtaccaaat
     1141 gcctgc
//
LOCUS       DAORYU010000365         1141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_85514_length_1141_cov_3.546961, whole genome shotgun sequence.
ACCESSION   DAORYU010000365 DAORYU010000000
VERSION     DAORYU010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85514_length_1141_cov_3.546961"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..>1141
                     /locus_tag="PLW07_08090"
     CDS             133..>1141
                     /locus_tag="PLW07_08090"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOL50531.1"
                     /translation="MSKLAILGGQPEVKNTSDDIFMRNITKEMEDAVLKVLHEWKMSA
                     TDITREFERAFADWHGMKYGLGCNTGTSALHCAMFGAGIGVGDEIICQSTTYWASCLP
                     VYSLGATVVFADIDPETLCINPDDIEHRITSRTKAIMVVHYAGMPADMDRIIPIAKKH
                     NIKVIEDVSHAHGALYKGKMVGTFGDVSGYSLMSGKGFAIGEAGIMLTDDRDIYERAI
                     LFGHYERHPEIQNPELAKFAGLPWGGYKYRMHQMSSAIGLVQIRQFQKDMVEIDRAMN
                     YFWDLLEGYPGIRAHRPPKNSGCTKGAWYASFGRYISEELEGLSISRFCEALRAEGVG
                     WA"
BASE COUNT          367 a          206 c          274 g          294 t
ORIGIN      
        1 aaaatttttc aacatgacca gttccctgtg tgcatcattc atacaaaaac aaatttttcc
       61 agtaagtcag caaaaaaaca cttgaaacac aggtgaaaat gaaatatgat aatcagaaac
      121 cagggggagt aaatgagtaa acttgcaatt ctgggcgggc aaccagaagt aaaaaatact
      181 tcagatgaca tttttatgcg caacattaca aaggaaatgg aagatgctgt cctgaaggtt
      241 cttcatgaat ggaaaatgtc agccacagac attaccagag aatttgaaag ggcttttgct
      301 gactggcatg gaatgaagta tggacttggc tgcaatacag gaacaagtgc tcttcattgt
      361 gcaatgtttg gagcaggaat tggagtaggg gatgaaataa tctgccagag tacaacatac
      421 tgggcaagtt gtctgcctgt ttactcactt ggtgcgacag tcgtgtttgc agatatagat
      481 cctgaaacat tatgtatcaa tccagacgat atagaacaca ggattacatc aaggacaaag
      541 gcaatcatgg ttgttcatta tgctggtatg cctgctgata tggacagaat aatcccgatt
      601 gcgaaaaaac ataacatcaa agttatagaa gatgtttcgc acgcgcatgg cgccctttac
      661 aaagggaaaa tggttggtac ctttggcgat gtttccggct attccctgat gagtggaaaa
      721 ggatttgcca ttggtgaagc aggaattatg ctgacagacg acagagatat ttatgaaagg
      781 gcgattcttt tcggtcatta tgaacgtcac cctgaaattc aaaatcctga acttgcaaaa
      841 tttgcaggcc ttccctgggg cggatacaaa tacaggatgc accagatgtc atctgcgatt
      901 ggccttgttc agatcaggca atttcaaaaa gatatggtag aaattgatag ggcaatgaat
      961 tatttctggg accttcttga aggatatcct ggaatccgtg cacacagacc accaaaaaat
     1021 tctggctgca caaaaggtgc ctggtacgct tcatttggga gatatatcag tgaagaactg
     1081 gaaggacttt ccatttcaag attctgcgag gcactgaggg cagaaggtgt gggatgggcg
     1141 c
//
LOCUS       DAORYU010000366         1136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_86059_length_1136_cov_4.719704, whole genome shotgun sequence.
ACCESSION   DAORYU010000366 DAORYU010000000
VERSION     DAORYU010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86059_length_1136_cov_4.719704"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /locus_tag="PLW07_08095"
     CDS             <1..857
                     /locus_tag="PLW07_08095"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOL50532.1"
                     /translation="FKLLPPMPEYWVFFDWAPLYRQGYSAVFNLMYLGALQCMVEICR
                     ILGKDKGFYQKQAELVEKRIIKYFWDEHDKVFYDGVDMETHGLIKKISQHTHALTILT
                     GLQPEFHRKFCEEILIPPMKKPALTDANIIEASPYFYFYVIEALKKIGGYETDIVQFI
                     KERWGSMLKQGSTTCWEIWNPKPGVISLCHAWSAHPSVHLMNIAGIVPVSPGWKKFLI
                     KPCLLDVNWLNLKIPVKTGDIVISMERHKDISSVSIQIPDGITCISTIAKSRKILKPG
                     IHKFKKTL"
     gene            924..1098
                     /gene="ssrS"
                     /locus_tag="PLW07_08100"
     ncRNA           924..1098
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PLW07_08100"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
BASE COUNT          360 a          207 c          256 g          313 t
ORIGIN      
        1 aattcaagct tctaccacca atgcctgaat actgggtatt ttttgattgg gcgcctcttt
       61 acaggcaggg atattcagca gtttttaatc ttatgtatct gggtgctttg caatgtatgg
      121 ttgagatttg cagaatactc gggaaggata agggttttta tcaaaaacag gcagaactgg
      181 ttgaaaaaag aatcataaag tatttctggg atgaacatga taaggtgttt tatgatggag
      241 tggatatgga aacacatgga ctgataaaaa aaatcagcca gcatacacat gctcttacga
      301 tactgacagg tttgcaacct gaattccata gaaaattctg tgaagaaatc ttaattccac
      361 caatgaaaaa accagccctc actgatgcaa acattattga agcgtcaccg tatttctatt
      421 tttatgtgat agaggcattg aaaaaaatcg gcggatatga aacagacatt gtgcaattca
      481 ttaaggaacg atggggtagt atgctcaagc agggcagcac aacatgctgg gaaatttgga
      541 atccgaaacc aggcgttata tccctgtgtc acgcatggag cgctcatcca tcagtccatc
      601 ttatgaatat cgccgggatt gttccggtat ctcctggatg gaaaaaattc ctgataaaac
      661 cctgccttct tgatgttaac tggttgaatt tgaaaattcc ggttaaaaca ggagatattg
      721 tcatttcaat ggaaaggcat aaagatatat cgtctgtgag tattcagata cctgatggaa
      781 tcacatgtat ttcaaccatt gctaaatcaa gaaaaatctt gaaaccaggc attcacaaat
      841 tcaaaaaaac tctgtaaccg aataggtaaa ttttctcttt tgtaaaagtg tatttacacc
      901 tttgattttt tctggtatta tagtattgcc tgtcccgtac gcaggcggag tgtgaaactt
      961 tgagccaaca ccaaaaaaaa gggagccgag tctgacatgc aagacatgcc ggaaacggaa
     1021 gtcgcggtgt tcagcaaggc gcccacctgt taatacgggt ctcaaaagtt gcaccgtcta
     1081 cggcgggacg ggtgaaatat ttatgggtag gtggccgagt ggaaatggca gcagac
//
LOCUS       DAORYU010000367         1133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_86369_length_1133_cov_4.875696, whole genome shotgun sequence.
ACCESSION   DAORYU010000367 DAORYU010000000
VERSION     DAORYU010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86369_length_1133_cov_4.875696"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..>1133
                     /locus_tag="PLW07_08105"
     CDS             87..>1133
                     /locus_tag="PLW07_08105"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOL50533.1"
                     /translation="MSTNDFEFKKNFAETLERHKTLQSAKRGCLIYTFLPEEEEIINS
                     VKIPPLEEIDFKKDVLLLPKLLCDREEKLYRCRSDYPDDSIPVLALRYGSGIFAGMIL
                     GSLRFGANTSWIEPVGKTIEETINFPWKKDNQFIDIALAGLQYATKRMKNKCYVYLSG
                     YHSPLELAFILRGSEFFLDVYKCPDKVHTLIKRCDEALKWVYQLIENNVKNENYGNIA
                     GFLWMERCLSFLSEDAAGLLSPQHYKEFGVPYTNEVFKRFGGGFLHVHTQSYHQMKNI
                     SSIEYLTTYNWRPDPNTPEAISILDKLLDGAKKKIVVIVADPRAIQEKIGLLSQGRFF
                     LLCYCKDRNEQQFIV"
BASE COUNT          384 a          200 c          222 g          327 t
ORIGIN      
        1 aaagggctgg acaagaacaa tcaccgatta tcagtggagt gcagccattt acgttgctat
       61 tgtcaatgaa ttgaaaaagg gtaatcatga gcacaaatga ttttgaattt aagaagaatt
      121 ttgctgaaac tcttgaaaga cataaaacac ttcaatcagc aaaaagaggg tgtctcattt
      181 acactttttt gccagaggaa gaagaaatta taaattcagt caaaattcct ccacttgaag
      241 aaattgattt caaaaaggat gtgctattgc ttccaaaact tctctgtgat agggaagaaa
      301 aactttacag atgcagaagt gattaccctg acgattcaat accagtactt gctttacggt
      361 atggttctgg tatatttgcg ggcatgattc ttggttcctt gaggtttggc gcaaacacat
      421 cctggataga acctgtggga aaaacaatag aagaaacaat aaactttcca tggaaaaagg
      481 ataatcaatt cattgatatt gctctcgccg ggcttcaata tgctacaaaa agaatgaaaa
      541 ataagtgtta tgtatatcta tccgggtatc attctcctct tgaactggca tttattttaa
      601 gaggctctga attttttctt gatgtctaca agtgccctga taaagtacat acattgataa
      661 aacgatgcga tgaagcactg aaatgggtat atcaactgat agaaaataac gttaaaaatg
      721 aaaattacgg aaacatagct ggctttttgt ggatggaaag atgcctctca tttttaagtg
      781 aagatgcagc agggcttctt tcaccacaac attacaaaga gtttggcgtg ccatatacca
      841 acgaggtttt taaaagattc ggcggcggtt ttcttcatgt ccatactcaa tcatatcatc
      901 aaatgaaaaa tatttccagt attgaatatc ttacgaccta taactggaga cctgatccaa
      961 acacaccgga agccatcagt attcttgaca aactgcttga tggagcaaaa aagaaaattg
     1021 ttgtaattgt tgcagacccc cgtgccattc aagaaaaaat cggcctccta tctcaaggga
     1081 gatttttctt gctttgctac tgcaaggaca ggaatgaaca gcaatttatc gtg
//
LOCUS       DAORYU010000368         1114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_88471_length_1114_cov_4.282342, whole genome shotgun sequence.
ACCESSION   DAORYU010000368 DAORYU010000000
VERSION     DAORYU010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88471_length_1114_cov_4.282342"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="PLW07_08110"
     CDS             <1..552
                     /locus_tag="PLW07_08110"
                     /inference="COORDINATES: protein motif:HMM:NF014707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HOL50534.1"
                     /translation="IAVHVCCGICAIKTFEFLEKNFEPAGYWYNPNIHPFEEYKKRFQ
                     TAGYVFERIGKKIEWDFSYDINQWFSMCLPVSKNKKARCAVCYQLRLEKTAQFAKQAG
                     IRIFTTTMFYSVHQDIETIKSIGIKIAQKYNLDFLPLDLRNHYQNGQEIARKWHLYRQ
                     KYCGCLFSELEREGFDYGSIEGK"
     gene            530..>1114
                     /locus_tag="PLW07_08115"
     CDS             530..>1114
                     /locus_tag="PLW07_08115"
                     /inference="COORDINATES: protein motif:HMM:NF020287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOL50535.1"
                     /translation="MEALKENDIVGLISEEGRKWFVKLEKKHFSTHEGLVDLQDIIGK
                     TPGDFVITNTNQKLYFFLPSLEELLLYYVKRSTQIIYPKDAGYLILKLGIKQGMKILE
                     VGTGSGAMTLILSYFAGSGGKVYTYEAREEFSKKAKNLIEQMQFSDRVVFYTKNIEDG
                     IEEHEFDCAVIDLKEPENYLGDIVECLSYGAVFGI"
BASE COUNT          372 a          159 c          246 g          337 t
ORIGIN      
        1 attgccgttc acgtttgttg tggaatttgc gcgataaaaa catttgaatt tctggaaaaa
       61 aattttgagc cggctggcta ctggtataat ccaaatattc atccatttga agaatataaa
      121 aaaaggtttc agacagcggg ctatgtgttt gaaagaatag ggaaaaaaat tgaatgggat
      181 ttttcttacg atatcaatca atggttttca atgtgcctgc ctgtatcaaa aaataaaaag
      241 gcaaggtgcg ctgtttgcta tcaattgcgt cttgaaaaaa cagcgcaatt tgcaaaacaa
      301 gccggaataa ggatttttac tacaaccatg ttctatagtg ttcatcagga cattgaaacc
      361 ataaaaagta taggcattaa aattgcccag aagtataatc ttgacttttt accacttgac
      421 ctcagaaatc attatcagaa cggtcaggag attgccagaa agtggcatct ttacagacag
      481 aaatactgcg gttgtttgtt cagtgaactt gaaagagagg gctttgatta tggaagcatt
      541 gaaggaaaat gatattgtgg gtttgatttc agaagaagga agaaaatggt ttgtgaaact
      601 tgagaaaaaa catttcagta ctcatgaagg gttggttgat ttacaggaca ttatcggtaa
      661 aacgcctggc gattttgtta tcacaaacac aaatcaaaaa ctttattttt ttcttccgtc
      721 tcttgaagaa cttctccttt actatgtgaa acgaagcacc cagattattt atcccaaaga
      781 tgccggttat ttgattttaa aattgggtat caaacaggga atgaagattc ttgaggtggg
      841 tactggttca ggcgcaatga cactgattct ttcgtatttt gcaggaagtg gtggcaaggt
      901 ttatacatat gaagcaaggg aggagttttc aaaaaaagca aaaaacctga ttgagcagat
      961 gcagttttct gacagggttg tgttttacac aaaaaacatt gaggatggaa tagaagaaca
     1021 tgagtttgat tgtgcagtga ttgatttgaa agaaccagaa aattaccttg gtgatattgt
     1081 cgagtgtctt tcatatggag cagtatttgg aata
//
LOCUS       DAORYU010000369         1108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_89122_length_1108_cov_3.987654, whole genome shotgun sequence.
ACCESSION   DAORYU010000369 DAORYU010000000
VERSION     DAORYU010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89122_length_1108_cov_3.987654"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..>1108
                     /locus_tag="PLW07_08120"
     CDS             12..>1108
                     /locus_tag="PLW07_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50536.1"
                     /translation="MFVTTEQTPLTLKNLRGTHFLVADGFGAGHMFTEQQQALFDKLV
                     DSEINRQQENVLFLQITAINKGNSPAYAFFKTPAPNFPQYSFENGMTVLSDDRICSVS
                     ILDEKPLCQEEFSLLLYPGQQATVDIRIPHKPVSKNRAKKLIQQSFEKKLEECRNFWN
                     SKLKQCPKIKLPQQRIEDMIYAGILHLDLIAYGIEPDQPVSACIGRYCPIGSESAPII
                     QFFDTMTWHTLAQRSLMYFVEKQHEDGFIQNFGGYMLETGAALWCMGEHFRYTRDKNW
                     VKKIKPCLLKAVDYIGKWIERNRDEKFKGICYGMIDGKVADPEDNYHIFMLNGYAYLG
                     LNRVAEMLSAIGEAKAKEIQDMAEQLKNNIR"
BASE COUNT          370 a          197 c          239 g          302 t
ORIGIN      
        1 atcattttac gatgtttgtt acaacagaac aaacaccatt gaccctgaaa aatctcaggg
       61 gcacacattt tcttgttgct gatggatttg gcgcaggaca catgtttaca gaacaacagc
      121 aggcattgtt tgataaacta gttgacagtg aaatcaacag gcagcaagaa aacgtgttgt
      181 ttttacagat aacagcaatc aataagggaa attcgcctgc atatgcattt tttaaaactc
      241 cagcaccaaa ttttccacag tattcttttg aaaatggcat gacagttcta tctgatgacc
      301 gtatctgtag tgtttcaatc cttgatgaaa agccgctgtg tcaggaagag ttttctctgc
      361 ttctttatcc aggacaacaa gcaactgttg atatcaggat tcctcacaag ccagtgtcaa
      421 aaaacagggc aaaaaaattg atacagcagt catttgaaaa aaaattggaa gaatgcagaa
      481 atttctggaa ttctaaattg aagcaatgtc caaagattaa acttccacaa cagcgtattg
      541 aagatatgat ttatgctgga attttgcacc ttgacttgat tgcgtacggg atagaaccag
      601 atcaaccagt tagtgcctgt attggaaggt attgtccgat tggttctgaa agtgctccaa
      661 tcatccagtt ttttgataca atgacctggc acacacttgc acagcgttcc ctgatgtact
      721 ttgtggaaaa acaacatgaa gatggtttta tccagaactt tggtggttat atgcttgaaa
      781 caggtgctgc actgtggtgc atgggtgaac atttcaggta tacccgcgac aaaaactggg
      841 taaaaaaaat caaaccatgc cttctgaaag cagttgatta cattggaaaa tggattgaga
      901 gaaacaggga tgaaaaattc aaaggtattt gctatggcat gattgatggc aaggtggcag
      961 acccagaaga caattatcac atttttatgc tcaatggata cgcatatctt ggtttgaaca
     1021 gggtggcaga aatgctttca gcaattggag aagcaaaggc aaaagaaatc caggatatgg
     1081 cagagcaact gaagaacaat atcaggga
//
LOCUS       DAORYU010000370         1105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_89513_length_1105_cov_2.858095, whole genome shotgun sequence.
ACCESSION   DAORYU010000370 DAORYU010000000
VERSION     DAORYU010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89513_length_1105_cov_2.858095"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PLW07_08125"
     CDS             complement(<1..391)
                     /locus_tag="PLW07_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50537.1"
                     /translation="MLKYFNLQKQMRETMMDYTKYRLKPKDELDDVLKEVSSLFVIWC
                     KKCYKVFETEEFSECDNFVELISGKDKINGCLGIDFLCNRFLTEKKIQNLPENSPIGV
                     ISCGLGIQTVAKLGENKRIRDIALADSV"
     gene            complement(405..>1105)
                     /locus_tag="PLW07_08130"
     CDS             complement(405..>1105)
                     /locus_tag="PLW07_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50538.1"
                     /translation="PELKQFFDFYTKFAQAGSGKFLIGSPAILPGHDLLSMLMGSEKF
                     MLTLMDKPVAMKSAIMKLARNWVILNKKINDIYLQYQEGYGVCWHNLWGPYSFVSTQS
                     DISCMLSREMFEQFVLDEIKLVGKEFGQVIYHLDGPGALHHVDTLCKVPEIKIIQWTP
                     GAGNTLSVEKWLPLYKKIQDAGKIVMAHIMPEEVEFFIKHLDAKLSYIWTHSRTKRDA
                     EILLTKVEKLCRSQ"
BASE COUNT          332 a          221 c          176 g          376 t
ORIGIN      
        1 gcacactatc ggccagagca atgtctctta tgcgtttgtt ttcacccaat ttggccactg
       61 tctgaatacc aagaccacaa gaaataacac caattggact attttcagga aggttttgaa
      121 tctttttttc agtaagaaaa cgattgcaaa gaaaatcaat tcccaggcaa ccgtttattt
      181 tatctttccc agatattaac tccacaaaat tatcacattc tgagaattct tctgtttcaa
      241 acaccttata acatttttta caccagataa caaaaagaga agaaacttct ttcagtacat
      301 catccagttc atcctttggc ttcaacctgt attttgtgta atccatcatt gtttctctca
      361 tctgtttctg cagattaaaa tattttaaca taaaatttcc agcattattg agaacggcat
      421 aatttttcga cctttgttaa aagaatctct gcatctcttt ttgtcctgga atgtgtccag
      481 atgtaagaaa gttttgcatc aagatgtttg atgaaaaatt caacttcttc tggcattata
      541 tgagccatta caatttttcc tgcgtcctgg atttttttgt ataatggaag ccatttttct
      601 acagataagg tatttcctgc tccaggagtc cattgtatta ttttaatttc tggaacttta
      661 cacagggtat ctacgtgatg taatgccccg ggtccatcaa gatgataaat cacctggcca
      721 aattcttttc caacaagttt aatttcatcc agtacaaact gctcaaacat ttcccttgaa
      781 agcatacatg atatatctga ttgtgtggaa acaaatgaat atggacccca tagattgtgc
      841 cagcaaactc cataaccttc ctgatattgc agataaatgt cattgatttt tttgttgaga
      901 ataacccagt ttcttgcaag tttcattatg gctgatttca ttgcgactgg cttatccatg
      961 agtgtaagca taaatttttc agaacccata agcattgaaa gaagatcgtg tcctggaagg
     1021 atcgctgggc tcccgataag aaattttccg gatcctgcct gagcaaattt cgtgtaaaaa
     1081 tcaaaaaatt gcttcaattc tggtg
//
LOCUS       DAORYU010000371         1100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_90144_length_1100_cov_2.809569, whole genome shotgun sequence.
ACCESSION   DAORYU010000371 DAORYU010000000
VERSION     DAORYU010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90144_length_1100_cov_2.809569"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..>1100)
                     /locus_tag="PLW07_08135"
     CDS             complement(71..>1100)
                     /locus_tag="PLW07_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50539.1"
                     /translation="LLRRTDITVPPLNVAGLWITVNTKGLPAGNYSGKVKISGKNVET
                     YEIVLTVNVADFKINPKKPVLVDGWTRPHEGEAYIWDFVEHGMNVWPGDITKKDMEKY
                     GIKMVRLSAWSANKAKEFVDHIKQLGLDYQDYFVGVLDEPGGKTETELKQYIEIAKAI
                     KEVDPNVRISFNPGEAATLPTFQILAPYCDFWVPYSLHVFSPYYENPKKKEIYLKKPW
                     MWYTTPCLWDKTAREPGIRIVPSQPGNCVGVAFFALNYPWRDQWDTAYEHINDASTMG
                     HVMSRHGPVPIIIWEEIREASQTANLAMMVRETLGVDTFDQVTDPDMQKLIKEGTDEQ
                     LIKWLQAH"
BASE COUNT          285 a          249 c          203 g          363 t
ORIGIN      
        1 gctgatatca atctcatcag caacatcaaa gcaccagtaa agcatcaatg aaccattgac
       61 aattttcatg ttaatgcgcc tggagccatt ttatcaactg ctcgtcagtt ccttctttta
      121 tcagtttttg catgtcaggg tcagtaacct ggtcaaaagt atcaacgcca agagtttccc
      181 ttaccatcat tgcaagattt gctgtttgag atgcctccct gatttcttcc catatgatta
      241 ttggaacagg cccatgtctg ctcattacat gccccatggt tgaagcatca ttgatatgtt
      301 cataggcagt atcccactgg tctctccatg gatagttgag ggcaaaaaaa gcaacgccca
      361 cgcaattgcc tggttgagaa ggtacaatcc tgatgcctgg ttcccttgca gttttgtccc
      421 acaggcatgg cgtggtgtac cacatccagg gttttttcag gtatatttct tttttcttcg
      481 ggttttcgta atatggagaa aatacgtgta aactgtatgg cacccagaaa tcgcagtatg
      541 gtgcaagtat ctggaatgtt ggaagggttg ctgcttcacc tggattaaag gaaattcgta
      601 cattcgggtc aacctctttg attgcctttg ctatctcaat atattgtttc agttcagttt
      661 ctgtttttcc acctggttca tcaagcacac caacaaagta atcctgataa tcaagtccaa
      721 gttgtttaat atggtcaaca aattcctttg ccttatttgc agaccatgct gacaatctta
      781 ccatcttaat tccgtatttc tccatatcct tctttgtaat atctccaggc cagacattca
      841 taccatgttc aacaaaatcc cagatatatg cttcgccttc atgaggtcgt gtccatccat
      901 ctacaagtac tggttttttg ggattaattt tgaagtcagc cacattcaca gtgagaacaa
      961 tttcataggt ttccacattt ttacctgaaa ttttcacttt gcctgaatag tttcctgcag
     1021 gtaatccttt tgtatttaca gtaatccaga gccctgcaac attcaatggt gggactgtga
     1081 tatcagtcct tcttaaaagg
//
LOCUS       DAORYU010000372         1088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_91621_length_1088_cov_6.569216, whole genome shotgun sequence.
ACCESSION   DAORYU010000372 DAORYU010000000
VERSION     DAORYU010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91621_length_1088_cov_6.569216"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="PLW07_08140"
     CDS             complement(<1..817)
                     /locus_tag="PLW07_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50540.1"
                     /translation="MNKKNAMSYVDESNENILIRYSCFRGKTNVDEYHFTLVPVSHGT
                     LSEQVALLDTSYKKILKKVGLNPSSAVFRRFFCSDLVNQYQTIKKYPVARTEKNHECA
                     LSFVSQAPYPEAKISLWAYHIVEKHPLLKSKDGSGLKLTRNSLTHFWDTGLISPEKPE
                     VDVQTTDILSKYKKLLSSRKMKLSDNVIRTWFFVKNIDTDYMEFAKDRKEFFEKNGLT
                     PATHFIASTGVEGSGTNIKARILLDAYSIAGIKPDQIQFLSDSRYLSPTYIYGV"
     gene            complement(810..>1088)
                     /locus_tag="PLW07_08145"
     CDS             complement(810..>1088)
                     /locus_tag="PLW07_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50541.1"
                     /translation="TIACTAESWFRPTSSMSLTFEINNAPEGIKLKNTSFDAGKFLLT
                     IEADKKLAGYRDNLIIQVFDEVTQPSGQKRKTFSGFLPAIAFEVVKNE"
BASE COUNT          308 a          199 c          208 g          373 t
ORIGIN      
        1 ttactccata aatgtatgtg gggctgagat acctggaatc tgaaagaaat tgtatctggt
       61 cgggcttgat gcctgcaatg gaataggcat ccaataaaat tctggctttg atgtttgttc
      121 cagacccttc aactccagta ctggcgataa aatgcgttgc tggagtaaga ccattttttt
      181 caaaaaactc ttttctatct tttgcgaatt ccatgtaatc tgtatcaatg ttttttacga
      241 aaaaccatgt gcggatgaca ttgtctgata gtttcatttt cctgcttgaa agaagttttt
      301 tatattttga aagaatatct gtggtttgaa catctacttc gggcttttca ggggatatca
      361 aaccagtatc ccagaaatgt gtgagagagt ttcttgttag tttcaagcca gacccgtctt
      421 ttgatttcaa cagaggatgc ttctcaacga tatgatacgc ccatagtgat attttggctt
      481 ctggatatgg ggcttgagaa acaaaggaca acgcacattc atgatttttt tcagtcctgg
      541 caacaggata ttttttgatt gtctgatact gatttacaag gtcactacaa aaaaatcttc
      601 tgaacacagc actgctgggg ttcaagccaa cctttttaag aatttttttg taggacgtat
      661 caagcaatgc aacctgttca gacagcgttc catgtgatac tggcacaagt gtaaagtgat
      721 actcatccac atttgttttt cctctgaaac aggaatatct tatcaagata ttttcattac
      781 tttcatctac atatgacatg gcattttttt tattcatttt tcacgacctc aaaagcaatt
      841 gcaggaagaa atcctgaaaa tgtttttctc ttctgtcctg aaggttgagt gacttcatca
      901 aatacctgaa taatcagatt gtcacgataa cctgcaagtt ttttgtctgc ctctattgtc
      961 aataaaaatt ttccagcatc aaacgaagta tttttcagtt tgatgccttc tggtgcattg
     1021 ttgatttcaa aagttaatga catggaagaa gtgggtctga accaggattc tgctgtacag
     1081 gcaatcgt
//
LOCUS       DAORYU010000373         1086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_91882_length_1086_cov_4.667313, whole genome shotgun sequence.
ACCESSION   DAORYU010000373 DAORYU010000000
VERSION     DAORYU010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91882_length_1086_cov_4.667313"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1086
                     /locus_tag="PLW07_08150"
     CDS             <1..>1086
                     /locus_tag="PLW07_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50542.1"
                     /translation="CCLLYSGIQPSLVEYEARITKLSAQIPQIIKAAEFAANHVLKYP
                     DTLINVPYDSQRTFSEELINRAGGLSIIYPEEWRKNQWTDHDIVLVSVRSWETDGETV
                     IKKIDEYRERNWKIIVFGSKKGMPKGLKCDYFIDNGAPSGQAVDGRINILANVTLGWM
                     WCCEYVAAMTRKGKIPGILISISLEEATDHNKKIQTPDGRLWIGDCPEKIPSGKLAKI
                     YLERVKKLISDLKSEKIQKQIEHASTMIAERMVEGKRVGVSGVGHVIVDEVKRDLKAP
                     WIGFQAVGYIRSRRNIFSRYLQPGDLLVWIAYIGLNSKYDNYGAKIKESGVDLITSFA
                     PDPDNPSVNETWAIAHIDQCWKKGDAE"
BASE COUNT          362 a          178 c          265 g          281 t
ORIGIN      
        1 gttgctgcct tctgtacagt ggaattcagc catctcttgt tgaatacgaa gcaaggatta
       61 caaaactttc tgctcagata ccccagataa taaaggcagc agaatttgca gcaaatcatg
      121 ttttgaaata ccctgacacc cttattaatg ttccctatga ctcgcagcga accttcagtg
      181 aagaattgat taacagagca ggcgggcttt caattatcta tcctgaagaa tggagaaaaa
      241 atcagtggac agaccatgac attgtgcttg tgtcagtgcg aagctgggag acagatggtg
      301 aaacagtaat caaaaaaatt gatgaataca gggaaaggaa ctggaagatt attgtctttg
      361 gttcaaaaaa gggaatgcca aagggattga agtgtgatta tttcatagac aatggcgcac
      421 catcaggtca ggcagttgat ggaagaatca atatcctggc aaatgttact cttggatgga
      481 tgtggtgctg cgaatatgtg gcagcaatga cgcgaaaggg aaaaattcca ggaattttga
      541 tcagtatctc actggaagaa gcaacagacc acaacaaaaa aattcagaca cctgatgggc
      601 ggctctggat tggagattgt cctgaaaaaa taccgtcagg aaaacttgca aaaatctatc
      661 ttgaaagggt gaaaaaactt atttctgacc tgaagtctga aaagattcaa aaacagattg
      721 aacatgcttc aacgatgatt gcagaaagaa tggtagaagg aaaaagggtt ggtgtttctg
      781 gcgtgggaca tgtcatcgtt gacgaagtga aaagggactt aaaagcgccg tggattgggt
      841 ttcaggcagt tggttatatc aggtcaagac gaaatatttt ttcaagatac cttcagccag
      901 gagatttgct tgtatggatt gcctatattg gtcttaattc aaagtacgat aattatggag
      961 caaagattaa ggaatcaggg gttgatttaa ttacaagttt tgcacctgac cctgataatc
     1021 catcagttaa tgaaacctgg gcaattgcgc atattgacca gtgctggaaa aagggtgatg
     1081 ctgaag
//
LOCUS       DAORYU010000374         1081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_92607_length_1081_cov_2.408382, whole genome shotgun sequence.
ACCESSION   DAORYU010000374 DAORYU010000000
VERSION     DAORYU010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92607_length_1081_cov_2.408382"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="PLW07_08155"
     CDS             complement(<1..696)
                     /locus_tag="PLW07_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50543.1"
                     /translation="MISVFGIKNMKQILYKLKSTPGPEILIRYVRNRSFECFHESERI
                     LKSIKNTTEWKKKKKEWLNCYQELLSPFIKDCPPLKPRIINTTKKDLYRIENVVFQSF
                     QGWGVNASIYIPSGKGPFPAVICPCGHSPKWELDYQVCATTLVLNGYIAVTFDAPVFG
                     EKNYLNDHFQNGVACYLTGIWSKVFFVADALRCIDYLATREDADIKNGVGMTGVSGGG
                     HTTITCAAIDERIS"
BASE COUNT          328 a          225 c          191 g          337 t
ORIGIN      
        1 acttattctt tcatcaattg ccgcgcaggt tatggtcgta tggcctcctc cagaaacacc
       61 ggtcattcca acgccatttt tgatgtctgc atcttctctt gtggctaagt aatcaataca
      121 tcgtaaagca tcggctacaa aaaatacctt tgaccatatt ccagtaagat aacaggcaac
      181 tccgttttga aaatggtcgt tcaaataatt tttctctccg aatacaggtg catcaaaggt
      241 taccgctatg tacccgttga gaacaagtgt tgtggcacac acctgataat caagttccca
      301 ttttggagaa tgaccgcatg gacagataac agcaggaaaa ggaccttttc ctgaggggat
      361 atagatactt gcattcactc cccaaccttg aaatgactgg aaaactacat tttctatcct
      421 gtataaatct ttttttgttg tgttaattat ccggggtttc aaaggaggac agtcttttat
      481 aaaaggcgaa agcagttctt gatagcagtt gagccactcc ttcttttttt tcttccattc
      541 agttgtgttc tttatacttt tgaggattct ttcactttca tgaaagcatt caaaagacct
      601 atttcttaca taccttatca gaatttctgg ccctggcgtt gatttcaatt tatacagtat
      661 ttgcttcata ttttttatac caaaaactga tatcatttcg cttcaagaga aactcaatga
      721 tatcacatat ttccaacgtt tcaaatgtct cctttaaacc cacctgcaga ataaaaaagg
      781 tttaattatt ataacaaaaa tcgtcctgca gtaaaggacc agaaaacagt tccagttttc
      841 aagcaatgtt ccagtagtgt caatagtgct ggattgatac ataaaaagac aatgggctgg
      901 catccaggca aacattttcc agttatgcca atcaagaaag aattgcgggt attgaaagag
      961 tgatatactg ctccccatta aggggaaatg gaaaaaaggt agaatagagt tgtttgaaag
     1021 ggaggcagta catttcaaat cccccgccaa cttcaggtct ccttcctttg caaatcccca
     1081 t
//
LOCUS       DAORYU010000375         1065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_94573_length_1065_cov_4.060396, whole genome shotgun sequence.
ACCESSION   DAORYU010000375 DAORYU010000000
VERSION     DAORYU010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94573_length_1065_cov_4.060396"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            219..>1065
                     /locus_tag="PLW07_08160"
     CDS             219..>1065
                     /locus_tag="PLW07_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat pathway signal protein"
                     /protein_id="HOL50544.1"
                     /translation="MIWASLIHLGFNMWEEEDAKPLYGMEKGRQRILNIRTAKPYLRF
                     DDELWNEILIKMKDAGMNMVVIDIGEGLRYESHPEISVKDAWSVTKMKREIAKLRSMG
                     IEPIPKLNFSTTHDAWLGPYSRCVSTDTYYAVCRDLIEEVCSIFDQSRFFHLGMDEET
                     ANHQRFALYAVMRQHTLWWHDLYFLIDQVEKQNVKAWVWSDYMWHHPDIFFKKMPKSV
                     VQSNWYYGKVFKSSINYVKAYLELEEHCYDQIPTGSNWSFEENFSNMVSYCSRRISQK
                     RLLGFL"
BASE COUNT          350 a          188 c          223 g          304 t
ORIGIN      
        1 caatcgtaac agaagattac acaaaaaaaa tcaggaaata tgcagaatct ctcggattta
       61 tgcctgaatc tgtgattttt taaaaacact ttttttgaaa ctattctgta gatactgcgt
      121 aaggacttca tacatcaagc caatacgtga aaattttaat cgttcgcttg tcagtttctt
      181 gcgcctgtca taaaataggg taataaaagg gagaatatat gatatgggcg agtttgattc
      241 atcttggttt taacatgtgg gaagaagaag acgcaaaacc attgtatgga atggaaaaag
      301 gacgacaaag aatccttaat atcagaacag caaaacctta tttaagattt gatgacgagt
      361 tgtggaatga aatccttata aagatgaaag atgcaggaat gaacatggtc gtcattgaca
      421 ttggcgaagg actgcgttat gaaagccatc cagaaatctc agtaaaagac gcatggtcag
      481 tgacaaaaat gaagcgtgag attgcaaaat tgcgttccat gggcattgaa ccaattccaa
      541 aactcaactt ttccaccacc catgatgcct ggcttggtcc atacagcaga tgcgtttcca
      601 ctgacacata ttatgcagta tgccgggatt tgattgaaga ggtctgttca atttttgatc
      661 aatcaaggtt ttttcatctt ggcatggacg aagaaacagc aaatcatcaa cgatttgctc
      721 tttatgcggt aatgcggcag catacactct ggtggcatga cctgtatttt ttgattgacc
      781 aggttgaaaa acaaaatgtg aaggcatggg tatggtctga ttacatgtgg caccatcctg
      841 acattttttt caaaaaaatg ccaaagagtg ttgtccagag caactggtac tatggtaagg
      901 tgttcaaatc cagtatcaat tatgtcaagg catatcttga acttgaagaa cattgctatg
      961 accagattcc cacaggaagc aactggtctt ttgaagaaaa tttcagtaac atggtttcct
     1021 attgcagccg aagaatatct caaaaacgat tgcttggctt tctcc
//
LOCUS       DAORYU010000376         1059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_95397_length_1059_cov_3.145418, whole genome shotgun sequence.
ACCESSION   DAORYU010000376 DAORYU010000000
VERSION     DAORYU010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95397_length_1059_cov_3.145418"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..>1059)
                     /locus_tag="PLW07_08165"
     CDS             complement(114..>1059)
                     /locus_tag="PLW07_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50545.1"
                     /translation="RYFIFASDRNGKVELYRMEVDGDEAVRITENFNQWYCWKVYNQE
                     VICNDGETFFAINIFDLSKRIISKKTDDNAIIYGTPVVSGDGNKIACLYQKDNSVWSI
                     VCAELKGSELFDVYKFDSKYHHVSHLQCAPAENFILTFAPLPDRQNNFNLPPSERARA
                     WKLDIEKGVAEPFLIMPVGYRATHEYWGQKNNERLYFHKKTVPNFTPTSIASIDIHGN
                     DLRVHFFSENRKLGHSFVSPDTRFMVSDVQEPGNNELYLIDMQTGKSEILCWPDSSCS
                     SQRNQLGHVHPSFSSKGNYIIYSSDRTVKTKVYIIPLK"
BASE COUNT          336 a          200 c          172 g          351 t
ORIGIN      
        1 ctggagtagt gtttttaatc agaatatcac aaatattttc ctgtgccatc agtgtccccc
       61 agagtaccaa aattataaaa attaaaaatt ttttcatatt tttccagagt agattatttt
      121 aatgggataa tgtaaacctt tgttttcact gtcctgtcag acgaataaat aatgtaatta
      181 ccctttgaac tgaaggatgg atgaacatga ccaagctgat ttctttgaga agaacacgac
      241 gaatcaggcc agcaaagtat ctctgatttt cctgtttgca tatcaataag atacagttca
      301 ttgtttcctg gttcctgaac atcagaaacc ataaatctgg tatcaggcga cacaaaagaa
      361 tgaccgagtt ttctgttttc agaaaagaaa tggaccctta aatcattgcc gtggatatct
      421 attgaagcaa tggaagtagg agtaaaattt ggaactgttt ttttgtggaa ataaagcctc
      481 tcattgtttt tctgtcccca gtactcgtgg gttgctcgat accccactgg cattattaga
      541 aatggttctg caacaccctt ttcaatatca agtttccatg cccttgcccg ctcagaaggt
      601 ggaaggttga aattgttctg cctatcaggt aatggcgcaa aagtaagtat gaaattttct
      661 gctggcgcgc attgtagatg agaaacatga tgatattttg aatcgaattt atacacgtca
      721 aataattctg acccttttaa ttctgcacaa acaatcgacc aaacagaatt atctttttga
      781 tataagcatg cgattttgtt tccatcacct gaaactacag gtgttccata aattattgca
      841 ttgtcatccg tcttttttga tataattctt tttgataaat caaaaatgtt aattgcaaaa
      901 aaggtttcac catcattaca tataacctct tgattataaa ctttccagca ataccattga
      961 ttaaagtttt cagtaatccg aacggcttca tccccatcaa cttccattct ataaagttca
     1021 actttcccat ttctgtcaga agcaaaaata aaatatcta
//
LOCUS       DAORYU010000377         1058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_95564_length_1058_cov_2.530409, whole genome shotgun sequence.
ACCESSION   DAORYU010000377 DAORYU010000000
VERSION     DAORYU010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95564_length_1058_cov_2.530409"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..654)
                     /locus_tag="PLW07_08170"
     CDS             complement(10..654)
                     /locus_tag="PLW07_08170"
                     /inference="COORDINATES: protein motif:HMM:NF002305.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosylase/DNA lyase"
                     /protein_id="HOL50546.1"
                     /translation="MKSKRKKEIGEILAAYEQIRNDIEKKFRQFENTGSRLNKKEIFR
                     ELCFCILTVQSRAENCWKCIELLDNTGLLEKGSFEEISNRLKGVRFHNNKAQRIIEAR
                     SSLETLMHLLKQENDSKKIRQWLVKNIKGIGMKEATHFLRNIGLSDDLAILDRHILRR
                     LNKLGIIKKIPESLSPKKYIEIEKKMQKFAKSIDVPASHLDFVFWYQETGRIFK"
     gene            complement(654..>1058)
                     /locus_tag="PLW07_08175"
     CDS             complement(654..>1058)
                     /locus_tag="PLW07_08175"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOL50547.1"
                     /translation="MFVEFYKPAFSIIISHLKKYGYLVQFHSCGAVSQLIPDFIEMGV
                     DILDPVQVSAQGMDIEILAAKFKDKICFHGGIDTQKLLPYGTPEQIRKEVRKIINLFQ
                     CRKIFVCPSQNFLPDIPTENLLAMYNTERTCN"
BASE COUNT          283 a          202 c          193 g          380 t
ORIGIN      
        1 ttgaaggtcc tatttaaata ttcttcctgt ttcctggtac cagaatacga aatcaagatg
       61 actggctgga acgtcaattg attttgcaaa tttctgcatt tttttttcaa tctcaatgta
      121 ttttttggga ctcagggatt caggaatttt tttaatgatg ccaagtttat tcaatcttct
      181 caaaatgtgc ctgtcaagaa ttgcaaggtc atccgataac ccgatatttc tgagaaaatg
      241 cgtggcttcc ttcattccaa ttcctttaat gtttttcaca agccactgtc tgattttttt
      301 agaatcattt tcctgtttca gcaggtgcat caatgtttcc agggatgagc gggcttcaat
      361 aattctttgt gccttgttgt tatgaaacct gacacccttc agccgattgc ttatttcttc
      421 aaaactgcct ttttcaagta agccagtatt atccagcagt tctatacatt tccaacagtt
      481 ttcggctcgt gattggactg tcaggatgca aaagcacagt tcacggaaga tttccttttt
      541 gtttagccgg ctgccagtgt tttcaaattg tctgaacttt ttttctatgt catttctgat
      601 ttgttcgtat gctgccagaa tttcaccgat ttcttttttt ctttttgatt tcatcagttg
      661 caggttcttt ctgtgttata cattgcaagc agattttcag tcgggatatc aggaagaaag
      721 ttttgagagg gacaaacaaa aattttcctg cattgaaaaa ggttgatgat ttttctgact
      781 tctttcctga tttgttcagg agttccatag ggtaaaagtt tctgggtatc aatgccaccg
      841 tgaaaacaga ttttatcttt aaattttgcc gccagtattt caatgtccat tccttgtgct
      901 gacacctgca cagggtcaag gatatccaca cccatttcaa taaagtcagg tatcaattga
      961 gaaacagcgc cgcatgaatg aaactggaca agatatccat attttttcaa atgagagata
     1021 atgatagaaa acgcaggttt gtaaaactca acaaacat
//
LOCUS       DAORYU010000378         1056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_95788_length_1056_cov_5.436563, whole genome shotgun sequence.
ACCESSION   DAORYU010000378 DAORYU010000000
VERSION     DAORYU010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95788_length_1056_cov_5.436563"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            258..>1056
                     /locus_tag="PLW07_08180"
     CDS             258..>1056
                     /locus_tag="PLW07_08180"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HOL50548.1"
                     /translation="MEKLLFLPVPREIKIANQFCNVPSHAGFFISGIDDKNYNFFSEY
                     IKKISQRFTKSQWKLNHRHPFFTLVCDDSLNIPKQGYKLTVSHDSIKAESSDASGAYY
                     ALCTLKQILIQKTNRLPVLTIEDYPDFKHRGVMIDISRSKVPKMETLFNLIDLLSGWK
                     INQLQLYTEHTFAYKGYEIVWQGRSPVTAEEIRKLDNYCKQRFVELVPNQNSFGHFHR
                     WLQHPQYRHLAEDADHPYTLCPVDSGSIQLLEDLYQQLLPNFSSNQFN"
BASE COUNT          358 a          198 c          185 g          315 t
ORIGIN      
        1 tgcctggttt gcctgttcaa tcacaagcgc ttctctaatt ccatggggat ccattcctgt
       61 tctccatttc gctgagaaaa tttgaacgat gataatttat tgtatagaag atacattgtc
      121 aaatattttg aatttgattg aaaatccaac tgtgatatca tctttaaaaa aagagaccag
      181 tcaatgaaaa aaccagcaga aaaatatgcg caggatttta agaagacaga aaaaatcaca
      241 tattagtggt gggaaaaatg gaaaaactat tgtttttgcc tgtgccacga gaaataaaaa
      301 tagcaaatca gttttgtaat gtgccttcgc atgcaggatt ttttatatca gggattgatg
      361 acaaaaatta caattttttc tctgaataca tcaaaaaaat ttcgcaaaga tttacaaaaa
      421 gccagtggaa attgaatcac aggcatcctt tttttactct cgtctgcgac gattcgttaa
      481 acattccaaa gcaaggatac aagttgacag tttctcatga ctcaataaaa gcagaaagtt
      541 cagatgcttc tggtgcctat tacgctttgt gcacattgaa acaaattctc atccagaaaa
      601 caaaccgtct tccagtgctt acaattgaag attatcctga tttcaagcac aggggtgtca
      661 tgatagatat cagtagaagt aaagttccaa aaatggaaac cctttttaat cttattgacc
      721 ttctttcagg gtggaaaatc aatcaacttc aactttacac agaacatacc tttgcttaca
      781 aaggatatga aattgtctgg caggggcgct ctccagtcac tgcagaagag attagaaaac
      841 ttgataacta ctgcaagcag agattcgttg aactggtacc aaatcaaaac agttttggcc
      901 attttcatag atggctacag catcctcaat acaggcatct tgcagaggac gcagatcatc
      961 catatacgtt gtgccctgta gattctggtt ccattcaatt acttgaagac ctttatcagc
     1021 aactgcttcc aaatttttca agtaatcaat ttaatg
//
LOCUS       DAORYU010000379         1054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_96129_length_1054_cov_1.826827, whole genome shotgun sequence.
ACCESSION   DAORYU010000379 DAORYU010000000
VERSION     DAORYU010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96129_length_1054_cov_1.826827"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1054)
                     /locus_tag="PLW07_08185"
     CDS             complement(<1..>1054)
                     /locus_tag="PLW07_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50549.1"
                     /translation="KQFLLAITYDVPDNLIANHYTPARMRDEMALLAKWGIKRIYWLD
                     YGKSFWKFDTAWEKNARKSIELCGDLLECAVTAAHSLGLEFFGIFKVFDMGFNRRFFD
                     KRAENLVKDIEEKYVWVMPELVQHQEFTMHANPAWIKAPEFPVTTLYLYSEKHIPDIQ
                     LKDVKIWVSRNNHKYSPYQKKFKVRTYVKFLPHCRWSPAGKIDENQKWKRKNYVLKIS
                     NLEITVPFIAIEINKKNLLFENFLYLIAECVDRKGRVFSLVPASAGDLRRGFVFNKTD
                     YPWANQTEGILSKVEWKNSLIGLTFKRAERLPTLLEPSFTGTKNIWLSRVERILASGA
                     EGIDIRILCHHNNCSEW"
BASE COUNT          299 a          235 c          163 g          357 t
ORIGIN      
        1 ccattcgcta caattattgt gatgacacaa aattcttata tctatccctt ctgccccact
       61 tgcaagtatt ctttcaaccc ttgaaagcca gatatttttt gttccggtaa aagatggttc
      121 aaggagagtt ggaagcctct ctgctctttt gaaggttaac ccaatcaaac tatttttcca
      181 ttcaacttta ctcaagattc cctctgtctg attcgcccag gggtagtcgg ttttattaaa
      241 aacaaaccct cttctcaaat ccccagctga tgcaggaaca agagaaaaca ctctgccttt
      301 tctatccaca cattcggcaa taaggtaaag aaaattctca aacaaaagat tttttttatt
      361 gatttcaatg gcaataaaag gaactgttat ttccagattt gatattttaa gaacatagtt
      421 ttttcttttc cacttctgat tctcgtctat tttacctgct ggtgaccaac gacagtgagg
      481 aagaaatttt acgtatgtcc tgactttgaa ttttttttga tagggactgt atttatggtt
      541 gtttctgctc acccatattt tcacatcttt caattgaatg tccggtatgt gtttttctga
      601 atacagatac agtgttgtta caggaaattc aggtgccttt atccaagcag gatttgcatg
      661 catagtaaat tcctgatgtt gtacaagttc aggcatcacc catacatatt tttcttctat
      721 gtctttcacc agattttctg cacgtttatc aaaaaatctc ctgttaaacc ccatatcaaa
      781 taccttaaaa attccgaaaa actccagtcc cagactatgt gccgctgtca ccgcacattc
      841 aagcaaatca ccacacaatt caattgattt cctcgcattt ttctcccagg cagtatcaaa
      901 tttccagaaa cttttgccat aatccagcca gtaaattctt tttatccccc actttgcaag
      961 aagtgccatt tcatcccgca tacgcgcagg tgtataatga ttggctatca gattatcggg
     1021 cacgtcatag gtaattgcga gaagaaattg cttt
//
LOCUS       DAORYU010000380         1051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_96457_length_1051_cov_5.772088, whole genome shotgun sequence.
ACCESSION   DAORYU010000380 DAORYU010000000
VERSION     DAORYU010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96457_length_1051_cov_5.772088"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="PLW07_08190"
     CDS             complement(<1..716)
                     /locus_tag="PLW07_08190"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOL50550.1"
                     /translation="MAIIKTQHLEKFLKEIPKVRILVVGDLILDHFVTGQVRRISPEA
                     PVPVVEVKKQMYRCGGAGNVCLNIAGLKGHVTICGVIGDDSNGAMLKDILETNNVGTF
                     LLPRKNFPTSIKTRIIAGSQQMIRVDNEQIEKLTQNEIEKMQHFFEKEIENFDAVIIS
                     DYGKGVIVPSLITVLVNLCRRYKKIITVDPKVNHFSYYRNVDCLTPNLMEASAGMHLP
                     EPSTEQEIVNLGNRIMKSLQS"
     gene            complement(722..>1051)
                     /locus_tag="PLW07_08195"
     CDS             complement(722..>1051)
                     /locus_tag="PLW07_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50551.1"
                     /translation="DSNKIGWLCRGIGLAVMSAIMFMRQQFLWWPLHPIGFVIGPVWL
                     MDWLWFSIFIAWLIKKIILKYGGAQAYEKSKYFFLGLPLGYYTCAGIWVFIDFIAKKH
                     GNVVFWI"
BASE COUNT          300 a          241 c          185 g          325 t
ORIGIN      
        1 gattgcaagc ttttcatgat tctgttgcca agatttacta tttcctgctc tgttgacggc
       61 tctggcaggt gcattccagc gcttgcttcc ataaggttcg gtgtcaggca atcaacattc
      121 ctgtaatagg aaaaatgatt aaccttcgga tcaactgtga taattttttt ataacggcgg
      181 cacaggttca ccagaaccgt aatgagagag ggaacaataa ctccttttcc atagtcagaa
      241 ataatcacag cgtcaaaatt ttcaatttct ttctcaaaaa aatgctgcat tttttctatt
      301 tcattctgtg tcaatttttc aatctgttcg ttgtccaccc gtatcatttg ctgcgagccc
      361 gcgataattc gtgttttgat gcttgttgga aagttttttc tgggaagcaa aaaggttcca
      421 acattatttg tttcaagaat gtctttcagc atagcgccat tggaatcgtc tccgataaca
      481 ccacaaatgg taacatgtcc tttgagccct gcaatattca agcacacatt tcctgcaccg
      541 ccgcaacgat acatttgttt ttttacttca acgaccggta caggcgcttc tggagaaatt
      601 cttcttacct gacctgttac aaaatggtca agtattaaat ctccgacaac aagaatcctg
      661 acctttggaa tttctttcag aaatttttca agatgttggg ttttaattat cgccatttat
      721 atcaaatcca gaaaaccacg tttccatgtt tttttgcaat aaaatcaatg aacacccaga
      781 ttcccgcgca tgtgtagtat ccgagcggta gccccaggaa aaagtatttg ctcttttcgt
      841 atgcctgcgc tccaccatac ttgagaataa tttttttaat cagccatgca ataaatatgg
      901 aaaaccataa ccagtccatc aaccatacag ggccaatcac aaagccaatt ggatgaagtg
      961 gccaccacag aaattgctgg cgcataaaca taatagcaga catcactgcc agtcctatcc
     1021 cgcgacacag ccatccgatt ttgtttgaat c
//
LOCUS       DAORYU010000381         1046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_97182_length_1046_cov_5.052472, whole genome shotgun sequence.
ACCESSION   DAORYU010000381 DAORYU010000000
VERSION     DAORYU010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97182_length_1046_cov_5.052472"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PLW07_08200"
     CDS             <1..212
                     /locus_tag="PLW07_08200"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOL50552.1"
                     /translation="IKSYKLLTGFRKLPEADIPAIADAILKVSQLCEDFPEIQELDIN
                     PLIVKNKGSGVVMVDARIILEWGEQ"
     gene            209..>1046
                     /locus_tag="PLW07_08205"
     CDS             209..>1046
                     /locus_tag="PLW07_08205"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOL50553.1"
                     /translation="MKNGRYLVGVVGAGGIGRYHIDGWKKMQDVVVYGISDVSTQYLD
                     EVSKKYEIPVAVKDYKKLINIEEIDIIDVCTPNAFHYPVTIAALKNGKHVICEKPLAT
                     SSKHVEKMIQAAKENKRKLMAAQNQRFTAPGKMTKGMIDQGIFGEIYFAQCYAVRRRE
                     LPPRDTFIKKELSGGGPMFDIGVHILDITYWFMGCPEVHSVKGVTFTKLAKNDDVTGS
                     WGQWDTKRFEVEDFASGFILFKDGRALQLTCSFLSNLPSNWSAHLYGTKAGMIWPEMK
                     MFS"
BASE COUNT          344 a          167 c          253 g          282 t
ORIGIN      
        1 aaatcaaaag ctataaactc ctcacaggtt tcagaaaact tcctgaagca gatatacctg
       61 caattgctga cgcaatcctg aaggtttctc aattatgtga agattttcct gaaatacagg
      121 aactggatat taacccgttg attgtgaaaa acaaaggtag tggcgtggtg atggtggacg
      181 caagaatcat actggaatgg ggggaacaat gaaaaatggc aggtatctcg tgggagttgt
      241 cggtgctgga ggtatcggca gatatcatat agacggctgg aagaaaatgc aggatgttgt
      301 ggtgtatgga atttcagatg tatcaacaca gtatcttgat gaagtgagta aaaaatacga
      361 aattcctgtt gcagtgaagg attacaagaa acttatcaat attgaagaaa ttgacatcat
      421 tgatgtgtgc actccgaatg cttttcatta tccagttaca atagccgcgt tgaaaaacgg
      481 gaaacatgtg atttgcgaaa agccccttgc taccagcagc aagcacgttg aaaaaatgat
      541 tcaggcagca aaagaaaaca aaaggaaatt gatggctgcg cagaatcaaa ggtttactgc
      601 acccgggaaa atgacaaagg gaatgattga ccagggtatt tttggagaaa tttattttgc
      661 gcagtgttat gctgtaagaa gaagggaact tccaccaaga gacacattta taaaaaaaga
      721 actttcaggt ggtggtccaa tgtttgatat tggagttcac attcttgaca tcacatactg
      781 gtttatgggc tgtccagagg ttcattctgt taaaggagta acatttacaa aacttgcaaa
      841 aaatgatgat gtaacaggtt cctggggaca gtgggacaca aaaagatttg aggttgagga
      901 ctttgcatcg ggttttattt tgttcaagga tggtagggca ttgcagttaa cctgcagttt
      961 cctttcaaat ttaccatcta actggtctgc tcacctttat ggcacaaagg caggcatgat
     1021 atggccagaa atgaaaatgt tttcag
//
LOCUS       DAORYU010000382         1046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_97282_length_1046_cov_1.711403, whole genome shotgun sequence.
ACCESSION   DAORYU010000382 DAORYU010000000
VERSION     DAORYU010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97282_length_1046_cov_1.711403"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="PLW07_08210"
     CDS             complement(<1..560)
                     /locus_tag="PLW07_08210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOL50554.1"
                     /translation="MLRELNNFPGWVPTIRDNSCILVVGASGGIGKSLVKMMLKSNVN
                     IGCHFATNKESLKEFECFENVSFFPKIFSNSSDCQSLIEEFYQWRNKIDALVVLSGGI
                     KNPVHWEQMSDTDWEADIFLNLSVPFYLAQAAIKKMKELKTPGKIILIGTESALHGGG
                     AFSMAYGVAKMGIECLVKGLARDVAK"
     gene            complement(545..>1046)
                     /locus_tag="PLW07_08215"
     CDS             complement(545..>1046)
                     /locus_tag="PLW07_08215"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOL50555.1"
                     /translation="TLIFGSPKARKRGNLPVEKAMEIAAAFFMEVAEKAKEKDVFLCI
                     EPLGPEETDFINSAYSALQLIKKVNHPYFQGHLDAKSLYASGEISYQTFQNFAPFLKH
                     FHVNEPELKPLCFSNLVDHCLIGKYLKDIGYTGYVSIEQRSVDSVDTLSVIKKSIEEM
                     KKCYVN"
BASE COUNT          312 a          237 c          156 g          341 t
ORIGIN      
        1 tattttgcaa catctctcgc cagtcctttc acaagacatt caatgcccat ctttgccacg
       61 ccgtacgcca ttgaaaatgc ccctcctcca tgcaaggcag attctgttcc tatcagaatg
      121 attttcccag gggtctttaa ttccttcatt ttcttaatgg ctgcctgcgc aagataaaaa
      181 ggcacactta aatttaaaaa tatgtctgct tcccaatctg tgtcactcat ctgttcccaa
      241 tgaacaggat tttttatacc acctgacaat acaacaagcg catcaatttt gtttctccat
      301 tggtaaaatt cttcaatgag agattgacaa tcagaactgt ttgagaaaat ctttgggaaa
      361 aagctgacat tttcaaaaca ttcaaattct ttcaatgatt ccttatttgt tgcaaaatga
      421 cagccaatat tgacattgct ttttaacatc attttcacaa gcgatttccc aatcccacca
      481 cttgctccaa caaccaatat gcaactattg tctcttattg ttggaaccca tcctggaaag
      541 ttattcagtt cacgtaacat tttttcatct cctctatact tttctttatt acactaaggg
      601 tatctacact atcaacactt ctctgctcaa tggacacata tccagtgtat ccaatatcct
      661 tcagatattt accaatgaga caatggtcaa ccagattaga aaaacaaagt ggtttaagtt
      721 caggttcatt cacgtgaaag tgcttgagaa aaggcgcaaa attttggaat gtttggtagg
      781 aaatttctcc agaagcatac agactctttg catccagatg tccctgaaaa taaggatgat
      841 ttactttctt tataagttgc agcgctgaat acgcagaatt gatgaaatca gtttcttcag
      901 gacccaaggg ttcgatacac aaaaaaacat ctttttcttt tgctttttca gcaacttcca
      961 tgaaaaaagc tgctgcaatt tccatcgcct tttcaacagg caaatttcct ctttttcttg
     1021 cttttggcga gccaaaaatc agtgtc
//
LOCUS       DAORYU010000383         1040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_98059_length_1040_cov_6.073096, whole genome shotgun sequence.
ACCESSION   DAORYU010000383 DAORYU010000000
VERSION     DAORYU010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98059_length_1040_cov_6.073096"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="PLW07_08220"
     CDS             complement(<1..826)
                     /locus_tag="PLW07_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50556.1"
                     /translation="MNKKTENVVSHFDESDKNILARYSCFKGKTEVDEYHFTFVPIVY
                     GTVSEQIELINTSYTKILKKLALDSNSAVFRRFFCSDLINQYQTIRKYSVVGSEKYDK
                     CAVSFVSQSPYPEAKIALWAYHIIDRNQPILKSIDGLGMKLKRGCLSHFWDTGIMSTE
                     EQSVRLQTMDILSLYKKILSHRNMKLADNVIRTWFFVKNIDTDYIEFAKARKEFFEKN
                     GLTPATHFIASTGVEGSAIDVRAKISMDAYSVAGIKPEQIQFLSDSRYLSPTYIYGV"
     gene            complement(819..>1040)
                     /locus_tag="PLW07_08225"
     CDS             complement(819..>1040)
                     /locus_tag="PLW07_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50557.1"
                     /translation="FEINNPPDGIKLKNVSFDGGKFLLTIEADKKLATYRDNLIIEVF
                     DEITQVSGQKRKTFSGFLPAIAFEVVKNE"
BASE COUNT          308 a          194 c          172 g          366 t
ORIGIN      
        1 ttactccata aatgtatgtg gggctgagat acctggaatc tgaaagaaat tgtatttgtt
       61 cgggtttgat acctgcgaca gaataagcat ccattgaaat tttcgctctg acatctattg
      121 cagacccttc aactccagtg ctggcgatga aatgtgttgc tggagtaagg ccattttttt
      181 caaaaaattc ttttctcgct ttcgcaaatt ctatgtaatc tgtgtcaatg tttttgacaa
      241 aaaaccatgt gcgtatgaca ttatccgcaa gtttcatgtt cctgtgggaa aggatctttt
      301 tgtacagtga aagaatatcc atggtttgta atcgcactga ttgttcttcg gtcgacatta
      361 ttccagtatc ccagaaatga gaaagacatc ctctttttaa tttcatacca agtccatcta
      421 ttgatttcaa aatgggttga tttctatcaa tgatatgata agcccacagt gctattttgg
      481 cttctggata tggcgactgt gagacaaagg acactgcaca tttatcatat ttttcacttc
      541 caacaacaga atattttctg attgtctgat attgattaat aaggtcgcta cagaaaaatc
      601 ttctaaacac agcgctattt gaatccagag cgagtttctt gagaattttc gtgtaagatg
      661 tgttaatcaa ttcaatttgc tcagatacag ttccataaac tattggcaca aatgtaaagt
      721 gatactcatc cacctctgtt tttcctttga aacaggaata tcttgccaga atatttttat
      781 cactttcatc aaaatgtgac acgacatttt ctgttttttt attcattttt cacaacctca
      841 aaagcaatcg caggaagaaa acccgaaaat gtttttcttt tctggcctga aacttgagta
      901 atttcatcaa acacttctat aatcagatta tcccgataag tggcaagttt tttgtctgct
      961 tctattgtca ataaaaattt tccaccatca aacgaaacat ttttcagttt tattccatct
     1021 ggaggattgt taatttcaaa
//
LOCUS       DAORYU010000384         1039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_98216_length_1039_cov_3.075203, whole genome shotgun sequence.
ACCESSION   DAORYU010000384 DAORYU010000000
VERSION     DAORYU010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98216_length_1039_cov_3.075203"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="PLW07_08230"
     CDS             <1..795
                     /locus_tag="PLW07_08230"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOL50558.1"
                     /translation="VKGTFDDCQALVKRAFNDPALSHLHLTSANSINFGRLLPQSVYY
                     FKAYAEIGYPVMLFSVPSGNFGNLMGGVLAREMGLPVEKFIVAVNENDEFPEFLKTGI
                     YNPVVPSRKCSSSAMNVGHPSNLARLIDLYGGWLTDERDQHGKVIRKGIMKRKPDLDL
                     MRKHFISFSITNHEVDDTIKKIYEEYSIIVEPHGAVSIAAALKANINAPVCCFETADP
                     AKFSEKIKQILNIEPPVPNSLLKLDTLEEKFIVIDNEYQQFLEILQ"
     gene            792..>1039
                     /locus_tag="PLW07_08235"
     CDS             792..>1039
                     /locus_tag="PLW07_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013413501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HOL50559.1"
                     /translation="MKYIILVPDGAADFPVEELNNRTPLEFANTPNLDYLAKIGRCGL
                     METIPPGFPAGSEVANLVILGYSPQQCYQGRGVLEAAS"
BASE COUNT          328 a          198 c          207 g          306 t
ORIGIN      
        1 gtcaaaggaa catttgatga ctgtcaggct ctcgtaaaaa gagcgtttaa tgaccccgcc
       61 ctgtcacacc tgcatttaac atctgccaac tctataaatt ttggaaggct tttacctcag
      121 tctgtttatt acttcaaagc atatgcagaa attggttatc ctgttatgct tttttccgta
      181 ccttccggta attttggaaa tttaatggga ggcgtgcttg caagggaaat gggtttaccg
      241 gtggaaaaat ttatcgtcgc ggtgaatgaa aatgatgaat tcccagaatt tcttaaaaca
      301 ggcatttaca atcctgttgt tccatcaaga aaatgttcat caagcgcaat gaatgtggga
      361 catccaagta accttgcacg tctcattgat ttatacggcg gctggcttac tgatgaaaga
      421 gatcaacatg gaaaggtcat aagaaaaggt attatgaaaa gaaaaccaga tttagacctt
      481 atgaggaaac attttatatc gttttcaatt accaatcacg aggtagatga cacaatcaaa
      541 aaaatatatg aagaatattc cattatcgta gaaccgcatg gtgcggtcag tatcgctgcg
      601 gcattgaaag caaatatcaa tgcgcctgtt tgttgttttg aaacagcaga ccctgcgaaa
      661 ttcagcgaaa aaataaaaca aattttgaac attgaaccgc ctgtaccgaa ttctcttttg
      721 aaacttgata cccttgaaga aaaatttatt gtcattgata acgaatacca acaatttctt
      781 gaaattttac agtgaaatac ataattcttg ttcctgatgg ggctgcggat tttcctgttg
      841 aagaattgaa caacagaacc cctcttgaat ttgcaaatac accaaatctg gactaccttg
      901 caaaaatcgg acgatgtggc ttaatggaaa caataccacc gggttttccg gctggttctg
      961 aagttgcaaa tctggtaata cttggttatt ctccccaaca atgttatcag ggacgcggag
     1021 ttcttgaggc ggcttccat
//
LOCUS       DAORYU010000385         1036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_98632_length_1036_cov_4.200815, whole genome shotgun sequence.
ACCESSION   DAORYU010000385 DAORYU010000000
VERSION     DAORYU010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98632_length_1036_cov_4.200815"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1036)
                     /locus_tag="PLW07_08240"
     CDS             complement(<1..>1036)
                     /locus_tag="PLW07_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HOL50560.1"
                     /translation="RKKGESKAAKEVDVVTTGTFGPMCSSSIYLSFGHPAPRIKIGGG
                     TCFLNGVPAYAGLAAVDVYLGATAVPPNDPGNLHYPGKFSYGGGFVIEELVNGKQVLL
                     EATSYGTDCYPRKQLKSYFSLKDINEAVLFNPRNCYQNYNVAVNASDRMLYTYMGILK
                     PNFGNANFCSAGILSPLLRDPEYYTTGIGTRIFLCGGIGYVIWHGTQHSPGVERTPEG
                     VPKRPAGTLAVIGDLKQMNGDFIRGASFVGYGVTLVVGIGIPIPVIDEKVAAWCGVPL
                     EKMCAPVVDYSSSYPERKPEVLAEVSYAELFSGTIKLGNKTISVGSLSSFLMARKIAE
                     TLKKWISEGKF"
BASE COUNT          297 a          253 c          179 g          307 t
ORIGIN      
        1 caaactttcc ttctgaaatc cattttttca gtgtttcagc aattttacga gccataagaa
       61 aactggatag agaacccaca gaaattgttt tgtttccaag tttgatcgtg cctgaaaaaa
      121 gctcagcata acttacttct gccagtactt ctggtttcct ttcaggataa cttgagctat
      181 agtcaacaac aggcgcacac attttttcaa gaggtacccc gcaccatgct gcaacttttt
      241 catctatcac tggtattgga atacctattc caacaacaag tgtcacccca tagccaacaa
      301 aagacgcgcc acgaataaag tctccgttca tctgctttaa atcaccgatg actgcaagtg
      361 tgccagctgg tctttttgga acaccttcag gtgttctttc aacaccagga ctgtgttgtg
      421 tcccatgcca gatcacatag ccaatacctc cgcagagaaa tatccttgtt cctatgccgg
      481 ttgtgtaata ttcagggtcc ctcagaagcg gcgaaagtat accagcagaa caaaaatttg
      541 cgtttccgaa atttggtttc aagattccca tataggtata cagcattcta tcagacgcat
      601 tgacagcaac attatagttc tgataacaat ttcttggatt aaacaaaact gcctcattga
      661 tatcttttag tgaaaaataa cttttcaatt gttttcttgg ataacaatct gtgccatacg
      721 atgttgcttc aagaagaacc tgtttcccat ttacaagttc ctcaatcaca aatccaccac
      781 cataactgaa tttccctgga taatgaagat ttccaggatc atttggtgga accgcggttg
      841 cgcccagata cacatccaca gcagccaggc ctgcataagc aggtactccg tttaaaaaac
      901 aggttccacc tccaattttg attcttggag caggatgtcc gaaactcaaa tagatgctgc
      961 ttgaacacat cggtccaaat gtgcctgttg tcacaacgtc aacttctttc gctgcttttg
     1021 actcaccttt tttcct
//
LOCUS       DAORYU010000386         1028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_99925_length_1028_cov_2.234327, whole genome shotgun sequence.
ACCESSION   DAORYU010000386 DAORYU010000000
VERSION     DAORYU010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99925_length_1028_cov_2.234327"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="PLW07_08245"
     CDS             complement(<1..219)
                     /locus_tag="PLW07_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HOL50561.1"
                     /translation="MIGRIREDIQTVFRKDPAARNLIEVLLCYPGLHAIWMYRISHYL
                     WKHRARTFARFISHIARFLTGIEIHPGAT"
     gene            complement(216..>1028)
                     /locus_tag="PLW07_08250"
     CDS             complement(216..>1028)
                     /locus_tag="PLW07_08250"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOL50562.1"
                     /translation="IKASPAAKRLANELGVEIEKVKGTGEGGRIEKKDVELYAQQQQT
                     TISEKVEKLSPVRKIIAERLSWSKQNIPHYYLSTKILMNNVEKLKNIFQAQGKKFTYT
                     DFIIFYTSRVLPDFPLINASFINDEIHYHATVDIGIAVNTENGLVVPVMKNLQDKTIE
                     EISATRSNLAEKVRSGKITKEDMENCRFVITNLGMFGVTQFQAIINPPGTAIMAVGSI
                     ETEPVVENNNVVIGKTMWVTLSLDHRIVDGAYGGAFLQKLKMVMENPGMLNL"
BASE COUNT          272 a          215 c          170 g          371 t
ORIGIN      
        1 ggttgctcca ggatggattt caattcctgt aagaaatcgt gcaatgtggg aaataaatct
       61 tgcaaatgtt cttgctctgt gtttccagag gtaatgtgat atgcgataca tccatatggc
      121 gtgaagtccc gggtaacaca atagcacttc aattaaattt cttgctgcag gatcttttct
      181 gaatactgtc tgaatatctt ctcttattct tcctatcata aatttaacat tccaggattt
      241 tccattacca ttttcaactt ctgtaaaaat gctccaccat atgctccatc aacgattctg
      301 tgatcaagtg acagagtaac ccacattgtt ttaccaataa caacgttgtt attttcaaca
      361 actggttctg tctcaataga accaactgcc attatcgcag tacctggagg attaatgatt
      421 gcctggaatt gagtcactcc aaacatgcca aggtttgtta tcacaaatct gcaattttcc
      481 atgtcttctt ttgtgatttt accgcttctg actttttccg caaggttgga cctggtcgct
      541 gatatttctt ctattgtttt atcctgcaaa ttcttcatga caggcacaac cagtccattt
      601 tcagtattga ccgcgatgcc tatgtcaaca gtagcatgat agtgaatttc gtcattgata
      661 aatgaagcat tgattaatgg aaaatcagga agcactctgg atgtatagaa aataataaaa
      721 tcagtatacg taaatttttt accctgtgct tgaaaaatgt tttttaactt ctccacatta
      781 ttcatcaaaa tttttgttga aagatagtaa tgagggatgt tttgcttact ccatgacaac
      841 cgttcagcga tgatttttct taccggggac aatttttcaa ccttctcaga aattgtggtc
      901 tgttgctgct gggcatatag ttcaacatct tttttctcta ttcttcctcc ctcacctgtt
      961 ccttttactt tttctatctc tacacccagt tcatttgcca gtctttttgc cgcaggactt
     1021 gcttttat
//
LOCUS       DAORYU010000387         1025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_100314_length_1025_cov_3.370103, whole genome shotgun
            sequence.
ACCESSION   DAORYU010000387 DAORYU010000000
VERSION     DAORYU010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100314_length_1025_cov_3.370103"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1025
                     /locus_tag="PLW07_08255"
     CDS             <1..>1025
                     /locus_tag="PLW07_08255"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOL50563.1"
                     /translation="TIETLKDQKTDDNNTKLFDKIYGCILAGAIGNAMGSPVECWFDW
                     EIDKKYPGGIKTVLDPRRLESEDDNQMAMLLVETYIERNGTPVMARHFGETWKNKLNR
                     DHFYPLCMGNAYNLIMSGWDPRITGHWNVVTGSTVMCMEPVGMYHIGDSEYARIDARA
                     ISYMYQRGLDVIAAEILVAAVSEALTPDATVDSVCNVAVKAAPDEKLNTFDKRDVRSC
                     REYLEKCLEIASKYTDVLAVRKELREKCLFYHPIDPLELLGIALAMFYIAKGDVRQAA
                     IGGTNIGRDSDTIAGRAAMLSGALSGAVSIPQEWINLFKPEVLEKIRKNAQIFVNNVI
                     SEKMKRL"
BASE COUNT          341 a          177 c          237 g          270 t
ORIGIN      
        1 taccatcgaa acattgaaag atcaaaaaac tgatgataac aacacaaaac tttttgacaa
       61 aatttatggt tgcatccttg ctggtgccat aggcaatgca atgggctcac cggttgagtg
      121 ctggtttgac tgggaaatag acaaaaaata cccgggtgga atcaaaactg tgcttgaccc
      181 cagacggctt gaatcagaag atgataacca gatggcaatg ttactggtag aaacctacat
      241 tgaaagaaat ggtacgcctg tcatggcaag gcattttggt gaaacatgga aaaataaatt
      301 gaatcgtgac catttttatc ctttgtgtat gggaaatgca tacaacttaa tcatgtcagg
      361 atgggacccg agaattacag gtcactggaa tgttgttact ggttccactg taatgtgtat
      421 ggaaccagtt ggtatgtatc atattggaga tagtgaatac gcaagaattg atgctcgtgc
      481 catttcatac atgtaccaga gaggacttga tgttattgcc gcagaaattc ttgttgcagc
      541 agtttcagaa gccctgacac cagatgcaac agttgatagc gtctgcaatg ttgcagtgaa
      601 ggcagcacca gatgaaaaac tgaacacatt tgataaaagg gatgtaagaa gttgcaggga
      661 atatcttgaa aaatgcctgg agattgcgtc aaaatatact gatgtgcttg ctgtgagaaa
      721 agaactcagg gaaaaatgcc tgttctatca tccaatagac ccccttgaac ttcttggaat
      781 agcgcttgcc atgttttata tcgcaaaagg agatgtcagg caggcagcaa taggtggtac
      841 aaatatcgga agggattcag acacaattgc tggaagagca gccatgcttt ctggcgcact
      901 aagtggagca gtttcaattc cacaggagtg gattaatctt ttcaaacctg aagtccttga
      961 aaaaatcaga aaaaatgctc aaatttttgt taataatgtc atttcagaaa aaatgaaaag
     1021 attga
//
LOCUS       DAORYU010000388         1023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_100599_length_1023_cov_3.965909, whole genome shotgun
            sequence.
ACCESSION   DAORYU010000388 DAORYU010000000
VERSION     DAORYU010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100599_length_1023_cov_3.965909"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..>1023
                     /locus_tag="PLW07_08260"
     CDS             33..>1023
                     /locus_tag="PLW07_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL50564.1"
                     /translation="MEYGFPVKPPPMQVRNIENIICTDYWHAGLRQDGSNLPDDLTVF
                     KPFRTLIENLKLVDATGVRIFSETSCCDNRIPWPTDLGPHTEKNILKTYVDALHKEGF
                     KVYFFTHSWITPFQKPDKMAPMPWRRWDYPYEQSDRFVDRDDVKQYYTEKYPCVISDN
                     DFHDKWLRLLSEAVAQGVDGLYIMPDEYYFKGHNLPKTNCPSCAVAFKNMFGYDSLPE
                     KPQDNEKYRKWELFEYEKLSSLFSDVASRLKKIKPNLTIFSNGNEAAVQIFNTRMEHG
                     IATDILGKDPGVNAGTVYGSTTLDTGGYVSLCRRFAAAFGKNRLEAAIQWLNIS"
BASE COUNT          317 a          220 c          224 g          262 t
ORIGIN      
        1 caatacagaa gaaaatacta ccaggaaaca ggatggaata tggattcccc gtgaaaccac
       61 ccccaatgca ggtgcgaaac attgaaaata ttatttgcac tgactactgg catgccggac
      121 ttcgccagga tggatcaaac cttcctgatg acctgaccgt ttttaagccg ttcagaaccc
      181 tgattgagaa cctgaaactt gtggacgcca caggtgtaag gattttcagt gaaacaagtt
      241 gctgcgacaa ccgcattccc tggcccacag accttggtcc tcacactgaa aaaaatattc
      301 tcaagacgta tgttgatgcc ttgcacaaag aaggattcaa ggtttatttc tttactcact
      361 cctggattac gccattccag aagccagaca aaatggctcc gatgccatgg agaagatggg
      421 actatcccta tgaacaaagc gacaggtttg ttgatcgtga cgatgtgaag caatactata
      481 ctgaaaaata tccgtgtgtc atcagcgata acgattttca cgacaaatgg ttgcgtcttc
      541 tgtcagaagc ggttgcccag ggcgttgatg gtctttacat catgccagat gaatattatt
      601 tcaaaggtca caatcttcca aaaacaaatt gtccatcctg tgctgtggct ttcaagaata
      661 tgtttggata tgactcgctt cctgaaaaac cacaagacaa tgaaaagtac agaaaatggg
      721 aactttttga gtatgaaaaa ctgtcttctc tgttcagtga tgttgcgtct cgtctaaaaa
      781 aaataaaacc aaatctaaca attttcagca atggaaacga agcagcagtt cagatattta
      841 atacccgcat ggaacacgga atcgctactg acatacttgg taaagacccg ggggttaacg
      901 caggaacagt atacggcagt accacgctgg atacaggcgg atatgtttct ctgtgcagga
      961 gatttgcggc tgcttttggg aaaaatcgac tggaagctgc cattcagtgg ctgaatattt
     1021 cat
//
LOCUS       DAORYU010000389         1011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1953
            NODE_102408_length_1011_cov_3.913180, whole genome shotgun
            sequence.
ACCESSION   DAORYU010000389 DAORYU010000000
VERSION     DAORYU010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863482
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:06:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,654
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,628
            CDSs (with protein)               :: 1,628
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 5 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102408_length_1011_cov_3.913180"
                     /isolate="AMR_MDS_1953"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..553
                     /locus_tag="PLW07_08265"
     CDS             62..553
                     /locus_tag="PLW07_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HOL50565.1"
                     /translation="MDKKFLVIFYSRTGTTKKIAREIANALSCDIEEIIDKKDRSGVR
                     GFMSGGRDAMKRELTEINDIEQDPSDYDVIVIGTPVWASNMTPAIRTYISKYKEKFKN
                     VAFFCTTGGTGIEKTLIEMEVLCGKKPVATVSLTVRQIKNSTYQSFLNKFVENLQQCE
                     ITN"
     gene            645..>1011
                     /locus_tag="PLW07_08270"
     CDS             645..>1011
                     /locus_tag="PLW07_08270"
                     /inference="COORDINATES: protein motif:HMM:NF027971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOL50566.1"
                     /translation="MFSINFYGAVENTTGSRHLLQINGRKFLIDCGIYQEREFLSRNW
                     EDFPVSPESIDAVFLTHAHLDHCGFLPRLVKNGFKGPVYATAPTIEIAKIALIDAAKL
                     YEEDAEKKRMRHLLSGRKVD"
BASE COUNT          360 a          155 c          211 g          285 t
ORIGIN      
        1 gatcaattga agccaatacg aattcctgat tttgtcagaa aagccattat ggaggcaaaa
       61 aatggataaa aagtttctgg tcatttttta ttcaagaacc ggaacaacaa aaaaaattgc
      121 tcgtgaaata gcaaatgcac tttcatgtga cattgaagaa atcattgata aaaaagatcg
      181 ctcgggtgtc aggggtttta tgtcgggcgg aagagatgcg atgaagcgcg aactcactga
      241 aatcaatgat attgaacaag acccatcaga ttatgatgtc attgtaatag gaactcctgt
      301 atgggcatct aatatgactc ctgccatcag aacatacatt tcaaagtata aagaaaaatt
      361 caaaaatgtg gcatttttct gtacaacagg ggggacaggt attgaaaaga cattgattga
      421 aatggaagtt ttgtgtggaa aaaaacctgt tgcaaccgtt tctttaaccg tgcgacagat
      481 taaaaatagt acttatcaga gttttttaaa taaatttgtg gaaaatttgc aacaatgcga
      541 aattacaaat taaattcaca ggtcaatgtt tttgaccttg ttattatttc agttaaaaat
      601 gaggtgaacc ccgaaaaaat cacaagacag gagacagatt acaaatgttc tcaataaatt
      661 tctatggagc cgtagaaaat actactggtt caagacatct tttgcagata aatggtagaa
      721 aatttttgat tgattgcgga atctatcagg aaagggaatt tttatcaaga aactgggaag
      781 attttcctgt aagtccagaa agtattgatg cagtgtttct tactcatgcc catcttgacc
      841 actgcggttt tcttccgagg ttagtaaaaa acggatttaa aggacctgtg tatgctactg
      901 cgcctaccat tgagattgca aagatcgcac ttattgatgc agcaaaactt tatgaggaag
      961 atgctgagaa aaaaagaatg aggcacttac tgagcggaag aaaggtggac c
//
