LOCUS       DAORZF010000001       104108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_139_length_104108_cov_366.738076, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000001 DAORZF010000000
VERSION     DAORZF010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 104108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 104108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..104108
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139_length_104108_cov_366.738076"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /gene="rlmD"
                     /locus_tag="PK015_00005"
     CDS             complement(<1..1060)
                     /gene="rlmD"
                     /locus_tag="PK015_00005"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOL32886.1"
                     /translation="MGDVVEVQLVTRKKDVAFAKVQKYITRGPGTIEPPCPSFGPPFF
                     CGGCDWLNLSYETQLKYKTELVREQIQSLNPRIDMPNTIASPVIQHYRNKAFMPVSKG
                     KNGLEFGIYERWSHKIVPHKTCFLHPSEFDLIAHRCLELMDNAKVQPYDETSHTGTLR
                     HIGFRCSKDKKQILMILVTRSAKLPFSNLLVKKLTSEFPQLSGIVQNINREKGNVILG
                     NEEKILYGNPYIYEEINNLKFRIHYKSFWQVNSAMLQLIIKNIQGKVQPEDTVLDTFS
                     GIGVLGLSLAAKVREVFCIEESSAAIDDGKFNCQLNDISNATFLNGKTEDTLPLILNS
                     PEKYTSKIPEVIILDPPRT"
     gene            complement(1143..2027)
                     /gene="htpX"
                     /locus_tag="PK015_00010"
     CDS             complement(1143..2027)
                     /gene="htpX"
                     /locus_tag="PK015_00010"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HOL32887.1"
                     /translation="MKAIIKRWGLFLAINFLVLLVLGIILQLLRINPSNVISLLIYCA
                     IFGFAGSFISLFLSKPMAKSSYRITPITRQNATGKALALYEMIEQMAAYKHIKMPEVG
                     IYPSPDNNAFATGASRNNSLIAFSSGIMENLNIDELGAVAGHEMTHITEGDMVTTTLL
                     MGTINTFVIFISHLLAIILSGGRNREQGFGRGTQFFFAILIQNILMIFANIAICAYSR
                     HREYVADAGSARLTSPVQMMIALEKISNKFVPPTKKDAYTIAKINYKGSATLFSTHPP
                     IEKRIERLRRMMEKEGSR"
     gene            complement(2043..4904)
                     /locus_tag="PK015_00015"
     CDS             complement(2043..4904)
                     /locus_tag="PK015_00015"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HOL32888.1"
                     /translation="MKRNLIVIALILLSTVILFAQPYAQTIFFSEYVEGSSYNKALEI
                     FNGTGAPVDLSQYTIKLASNGQTWSTSNYLTPTAILQHNDVFVIAHPQASAAILSVAD
                     VTSNVVNYNGNDCVGLFLGTTLIDIIGIYQNDPGTNSGWNVAGVPAATLNHTLIRKPT
                     VIQGNTDWTISAGTNADDSEWIVHPQDYITDLGMHTFTPGGGDITAPPVFNPAGGIYT
                     SPVTVTITCATPNASIYYTLDGSVPTPSSTLYTAPITISVTTTLKAIAIAPGMSNSSV
                     TTAIYTFPLTVPNLSTLRSQPADGVTVYQVTGEVILTFQQSFRNQKYVQDDDAGILID
                     DNNGVITTTYNIGDGITGLTGKISEYGGMLQFVPVINAGPATSTNNLIVPIIATYQQL
                     IDDFDTFESRVVKVLSVNFTNPSGNFANGTVYPTSDPTASYNIRTTFYDVDYINTPIP
                     TTTKDITGIPNSRTDGDYFTPRSLSDFSDPAGPVAAPVFSHPSGYYWESFFLTITCSN
                     PNANIYYTLDGSTPNLNSTLYTAPLTISSTTTVKAIAYISPAEYSAVTSATYTFPVMV
                     NNLAALRQSPLNGLYRLTGEVLVTFTQSYRHQKFVQDSTAGILIDDNNGIITTVYQIG
                     DGISHLTGTLTEFGGMLELVPVVDPGTPSSTGNTITPIDIDILQFLDDFETYESRLVR
                     ISDIRFFEPTGTFANGLAYLLIDATGDNTAFFRTTFYDVDYIGLPVYSYLLNIVGIPN
                     SRTDGNYYTARYKADFQISEPLPPLVFTHTILTPSALEITVGFETEANVVLPEGLTGY
                     KIYRDNEVVHTAPASMMITWQDISIPLGTHIYYATALYGNVESDTTSSFTFIVSGIDD
                     NPPLTPHTYLGQNYPNPFNPSTEIHFSLAEPGKVIIDIYNQKGELVRNLLNGNFTAGT
                     NKVFWDGLDNEGKIVSSGVYYYRMRNGAYSSTRKMLLLK"
     gene            6056..8539
                     /gene="leuS"
                     /locus_tag="PK015_00020"
     CDS             6056..8539
                     /gene="leuS"
                     /locus_tag="PK015_00020"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOL32889.1"
                     /translation="MEYPFSKIEPKWQKIWREKGIFHPEDFPSQPKYYVLSMFPYPSG
                     ILHCGHVSNYAISDAIARLKMMDGYAVLQPMGFDSFGMPAENYAIEHNSHPRITTEEN
                     IQAMHRQFDSLGFGLDWEREISTCRPDYYVWGQYLFKKLYEKGLVYRKKSWQNWCANC
                     QTILANEQVVNGTCWRCHNPVTQKEQEQWFFRITAYAEELLDFSKVIDWPERVITMQK
                     NWIGKSEGTLIDFPLEDGTDSIQVFTTRPDTVYGVTFMALPPEHPLVQKWLKEEPENR
                     ALKEFCERVINEDKISREAVDTIKEGMFSGRYCLNPLNGEKIQIWVTNYVVMDYGTGA
                     VMAVPAHDERDFEFARKYGIPIKLVIQNPERTLNLETMENAYIEPGIMVNSAPFDGMY
                     SEEAKIAISKFLEEKGYGKITSAYRLRDWCISRQRYWGNPIPIIYCPNCGIVLVPDED
                     LPVLLPDNVQVGKTTRNPLLSVPEWYNVKCPQCGADARRETDTMDTFVDSSWYYARYT
                     DPHNDKRPFDPDKANFWLPVDQYIGGIEHACMHLLYARFICKFMRDLGWLKYDEPFLR
                     LLTQGMVLKDGAKMSKSKGNVVDPNYIIDRYGADTLRVFLLFASPPDKDVEWSDEGIM
                     GAFRFLNRIWRLIDENLDIIQKGVKIEPDYTQISTKMRNLIYSLHKTIKKWREDCLDR
                     MQFNTAIAAVMEHLNNCLAIKEVNELSDNEQAVFAQACIVFPPLIYPFAPHIAEELWQ
                     KLGNEKLLHESGLPEYSERFLELDKITYVIQVNGKLRGKIEVDPDISEEELKKLALEV
                     ENVKRHISNQEIKKIIVIPGKMVSIAVGK"
     gene            8589..8921
                     /locus_tag="PK015_00025"
     CDS             8589..8921
                     /locus_tag="PK015_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL32890.1"
                     /translation="MCCHNEHHCYDDYCCCCGHHHSHFNHPHQHCCYGHHYYNHQRDE
                     DYCKCEEGDFEHHKEHHHHHYGQHFAPYYECCSQISKEKLLKAKKHLERIIQWIDQQL
                     AEEEESGN"
     gene            8921..9775
                     /locus_tag="PK015_00030"
     CDS             8921..9775
                     /locus_tag="PK015_00030"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOL32891.1"
                     /translation="MEANQEKEIQANLAKIHHRIVVLSGKGGVGKSFIATNLAYGLAL
                     QGKTVGLLDVDIHGPSIAKLTNIEGKPIPIDQESGKPTPIPVLSNLYVLTLASILDSE
                     STAVIWRGPMKLALIRQFFSDFEWKELDYLIVDCPPGTGDEPLTVIQTLNKVDGVVIV
                     TTPQDLAILDVKKTVDFAEKLNVPILGLVENMKYFRCPHCGKTTQIFTGNQLVDLVFE
                     YSLDILAELEIDPDISKSADEGKPYIYFYNKKDSANALMNAVLKIMEKVEGINNPDKR
                     DKAFDNYV"
     gene            9827..10039
                     /gene="rpmE"
                     /locus_tag="PK015_00035"
     CDS             9827..10039
                     /gene="rpmE"
                     /locus_tag="PK015_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012439895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOL32892.1"
                     /translation="MKKGIHPKYDIATVHCVCGNTFQTRSTKGDLQVEICNKCHPFYT
                     GKQKIVDRAGRVEKFIKKYNLKTDEK"
     gene            10130..11113
                     /locus_tag="PK015_00040"
     CDS             10130..11113
                     /locus_tag="PK015_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HOL32893.1"
                     /translation="MEQKKEISVGGQAVIEGVMMRGPDVIATAIRRKDGTIELFREHF
                     ETKAKKGTFLGLPIIRGFVSLIEMLIIGMKTITLSANRAELDLKENAPNPAKASTEKS
                     KTVQQLENLFSYVIAFALAFLLFGWLPYWLADLVGFSRQNFFFNLFAGVMRAVFFVVY
                     IWLISKMKDIHRIFQYHGAEHKNVNAYEKGIPLEIPQIQANSTIHPRCGTSFIFFVLL
                     LSIIIFSLADTLVSAFIIHGKIPLATRLAYHIVILLPLIGGVSYEILKFSGKNINHPL
                     VKILTVPGMALQKITTQPPDDSMIEVGLVAMKAALGMDLDGHNVQIIQDVT"
     gene            11103..12170
                     /gene="prfA"
                     /locus_tag="PK015_00045"
     CDS             11103..12170
                     /gene="prfA"
                     /locus_tag="PK015_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOL32894.1"
                     /translation="MLPEEKLNSYKKELEELQKTLSDPSIINDTNRYKALRRRYKELE
                     EICSEWDNYQKLEKQLADTNSLLQTESDPELEALAKEELEELKNKFQTSEEKIRELLI
                     PQDPNDHKNAIVEIRAGTGGEEAALFAADLFRMYSYYAEKKGWQIQVLSANETELGGY
                     KEIVFLLTGDDVYGHMRFESGVHRVQRIPVTESNGRIHTSAVTVAVLPEAEDIDIDID
                     EKDLRIDVYRSTGHGGQSVNTTDSAVRITHIPTGIVVTCQDEKSQIKNKAKALKVLRS
                     RLLDAEISRQEQEIARSRKAQVGTGDRSAKIRTYNFPQSRVTDHRINLTSYDLESFMN
                     GEIDDFIQALRLAWRNEKVKE"
     gene            12171..13436
                     /locus_tag="PK015_00050"
     CDS             12171..13436
                     /locus_tag="PK015_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOL32895.1"
                     /translation="MSILQIILWCLIFLVLMLFSFLFAGFENGVISINQLELESKAKK
                     DKSAAKLLAMVRQPDKVLGTTLLGNNIVNILFASLATYVVDRYIVSFNARYTSLIVGA
                     IVLIFCEVVPKTLFREYSDVLVPAVAPFMRGVYAILKPLVTCVSWMNTRLRNMLKITE
                     GNSFNYLTKDDLTYLLSVTEADASEEPQIEMIEDALDFTEQVAKDIMVPRTELVAIPA
                     TATVSEAIEIAREEGFTRYPVYGKDIDDIVGILIIYDFLKKECSPDTPVSKLTHKPFF
                     TPENINLSVLLRQMQKYHRSIAIVVDAYGGTSGIVTMEDILEEIVGEIADEYDEEEEI
                     EEVEQLTPDTWLVPGDMEIDRLADEYNIQLPEGDYQTVAGLILDRLAKIPHQGQIINI
                     EGYRIQVLQVTDRKILKVKIHRIKPRGNV"
     gene            13433..15106
                     /gene="ftcD"
                     /locus_tag="PK015_00055"
     CDS             13433..15106
                     /gene="ftcD"
                     /locus_tag="PK015_00055"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HOL32896.1"
                     /translation="MKLMECVPNFSEGRDQSVIDAIANAIKSVKNVVLLDVDPGADTN
                     RTVFTMAGEPEAVLEAAFQAIKKASELIDMSKHHGEHPRMGATDVCPFIPISDMTMEE
                     CVEYARRLGKRVGEELGIPVYLYEYAATKEEWRNLSNIRAGEYEALPEKAKDPAWKPD
                     FGPHIFNAKSGATAIGAREFLIAYNINLNTRDKRKASELAAIIRESGRLAKDEKGRII
                     KDEQGNKVIIPGLFQHCKAVGWYIESYKRAQISINLTNYKITPPHIVLEKVRELASER
                     GIVVTGSELVGLIPKAAMVNAGKFYLERMGESTGLPERMIMETAIQSMGLAELTPFDL
                     DKKVIEYAIAKKDRLSAMSLESFADLLSTDTPAPGGGSVAAFCAALSAALTAMVSNLT
                     INKKGYESVQNQVREIAPQAQDIKLQAIQLIDEDTDAFNAMMDASRLPKKTEEEIALR
                     EQKIEEATKQAILVPFHTLEIALQALKLADEVAKVGNMNALSDAGTAALTALTAAKAA
                     NYNILINLPQIKDEAFKEDINNRAMNLLQESETLASQIESFVSDRLKNA"
     gene            15146..15547
                     /locus_tag="PK015_00060"
     CDS             15146..15547
                     /locus_tag="PK015_00060"
                     /inference="COORDINATES: protein motif:HMM:NF039183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HOL32897.1"
                     /translation="MKASFIHLFSRLNLLILQFIIFVILNILDGHSTWKVLYPDKHHR
                     EMNPLARWVFRKLGVTKGIITYKTVLILIIGILFGHYAKKDTFSLNVILSVANIVFLI
                     TVVHNYKVYRKIHHSQRKSDKTSELDGEEIS"
     gene            15653..16612
                     /locus_tag="PK015_00065"
     CDS             15653..16612
                     /locus_tag="PK015_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOL32898.1"
                     /translation="MKILITGGAGFIGSHLAERLVEEGNQVVVVDNLSTGRLENIESI
                     KANGNFHYTIGSILNRELLEKLMEGVDQVYHLAAAVGVKYIIENPLLSLRTNILGTEN
                     VLELANKYKAKVLITSTSEIYGKSDKVPFHEDDDRLLGPTRISRWGYSCSKAIDEFMA
                     LAYFREKRLPVVIVRCFNTVGPRQTGQYGMVLPKFIKAALLDQPIVVYGTGKQTRCFA
                     DVSDVVGAFIKLMNTPKCVGEIFNVGTTESISIEDLAKKVKEMCDSKSRIEYMSYEEA
                     FEEGFEDMMNRMPCLDKVKEYIGYEPKYSLDDIIRRMIEYYEK"
     gene            16602..17957
                     /locus_tag="PK015_00070"
     CDS             16602..17957
                     /locus_tag="PK015_00070"
                     /inference="COORDINATES: protein motif:HMM:NF022007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOL32899.1"
                     /translation="MKSKVICIILLSLSVFIAAQVPTTYAPTNLISINVTGFVDSPGT
                     YQLSSISRLSDALKLAGTPSPIILEKGPTYIPRLYASEEKSVSPKDSLFLNFQALRSV
                     KLTRGKETKTYDYMKFVRMGDLEQNPILRDGDVINVGAVHTTVTIVGEVYYPGEYEYI
                     AGDRLSDLLNLAQGFTLSADRKIVNIYRYKENLKDFDLITVDLRTHKPEDIIIYPYDR
                     ITINRDTEYRRGWNIIVEGNVKSPGKYYIGDNTTLYDILLLCGGPNSRGDLRNAIYAN
                     LPNTEKVDPEFERLKKMDITQMSIIEYHYLQNRLRQFPGRYSIDVSRTWESKGSECNP
                     VLQDGDYLYVPLKMDMVAVTGQVVNPGLIPWVEGKTWDYYVKSAGGYTNNKSFNGVRI
                     IDAQSGNWVRPSSKTVLYPGDTVFVAAKTEYDAWTRFKDIMLITTQIMTIILGIHTLT
                     N"
     assembly_gap    18073..18172
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18173..19231
                     /locus_tag="PK015_00075"
     CDS             <18173..19231
                     /locus_tag="PK015_00075"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOL32900.1"
                     /translation="SKVKAAIDEVIAENDFILGHQVKEFEDKMANYLGIKYAIGCASG
                     TDALVLAVQATGIGADDEVITTPFTFFATASSIWRNGAKPRFVDINPDTFNLDVEKLE
                     AAITDKTRAIMPVHLFGQACDMTRIMEIAEKYNLKVIEDNAQGIGCTWDGKMSCSFGD
                     VGTLSFFPSKNLGAMGDGGMCLTNDEELALKLRQLRVHGENPKYYHKWVGLNSRLDTI
                     QAAVLGVKLDYLSQWSEGRRANAAFYDEQLADIPQVKTPYIHPSAVSIYNQYTIYCEN
                     RDALLHHLKEKEIGCAIYYPLPLHLQECFAELGYKKGDFPMAEKASQSVLSLPIYPEL
                     TDAQKSYVVEAIKEFYQG"
     gene            19237..20028
                     /locus_tag="PK015_00080"
     CDS             19237..20028
                     /locus_tag="PK015_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOL32901.1"
                     /translation="MNIIVCVKQVPNTTEIKIDPVTNTLIREGVESILNPFDAYAVEE
                     AVRMKERYGGIVTAICMGPNQAEETLRETVAVGVDNIILLSDRRFAGADTLATSLTLA
                     TAIKKIGDYDLIFTGQQAIDGDTGQVGPGIASHLDICQCCFVRKIDEVNEGTITLQRL
                     MEDGSDKLKLKLPAVISVVKEINTPRLPSLRGKRNARSVPLTVWNADDLGLDEKEIGL
                     NGSPTQVIKIFTPKHEKKTEKYVVSPEEAADLIVKKLDEITRGAK"
     gene            20028..20846
                     /gene="lgt"
                     /locus_tag="PK015_00085"
     CDS             20028..20846
                     /gene="lgt"
                     /locus_tag="PK015_00085"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOL32902.1"
                     /translation="MLPYPNIDPVMAQFTIGGINFSIRWYGFFYVLSFILGYIFLWKL
                     LKYRKVKITRDQYDGIIFSIMLGVILGGRLGYVLFYNLPYYLRHPVEILYVWEGGMSF
                     HGGALGVIIACLIYMRRQKIPFYSVADCAMPMVAVGLGLGRLGNFINGELWGKVTNLP
                     WGMVFPDAGPLPRHPTQLYELFFEGIVLGVLSYILLKKTKKEGLVFWSFIGLYGIFRF
                     LIEFIRVPDDLEIYDKIGYFLGFMTIGQILSVIMIIAAAIGIWTLYRKKPEVAL"
     gene            20843..21538
                     /locus_tag="PK015_00090"
     CDS             20843..21538
                     /locus_tag="PK015_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL32903.1"
                     /translation="MKHPVFLFSLTIVLLTVVSCASMQPVDKTQLLRTEWERWQNFEA
                     QGVVQLTNSGLTMRRMFVLSKSPEAMRFDILSGGVLAANPKPLISVYYGDYLAIQCPQ
                     FPKIEILAQMPIFPEKLPIYDIDRDSLFTVFADSIINHQELNLEGLQLKFNSAMQLER
                     IIEPKSGMEININRNSQGDPDKVKLTISENTYLVLLIDSISYGNAHIEPLPPNPKVEL
                     PEDWKQMFKSLLE"
     gene            21542..22837
                     /gene="purB"
                     /locus_tag="PK015_00095"
     CDS             21542..22837
                     /gene="purB"
                     /locus_tag="PK015_00095"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOL32904.1"
                     /translation="MIKRYSLPEMEHIWTQENRYECWLEVELAAARAMHELDIIPDAD
                     WEIIAEKADFNSERIDEIEAVTHHDVIAFLTNVAEYVGEPARWIHYGLTSSDVLDTAT
                     ALQLKRSGELILTELLRLAETLKLKAREHRRTLCMGRSHGIHAEPTCFGLKFALWYDE
                     AQRNVQRLQNAIDTVAVGQFSGAVGNFAHIDPRVEELACKYLDLKPVNISTQVIQRDR
                     YAYFLAELALIGALIEKIALEIRHLQRTEVQEVEENFSPGQKGSSAMPHKRNPIVSEQ
                     LCGLSRVLRSNLIAALENIPLWHERDISHSSVERIILPDSCILVHYMLSKCCSLISNL
                     VVYTENMKANMELTHGLVFSQAVLLHLVQKGLSREVAYTIVQSDAMECANTGKDFKEY
                     VLADSRITSLLPEAEIKDIFNYERYLRNVDFIYKRCGII"
     gene            22870..23307
                     /locus_tag="PK015_00100"
     CDS             22870..23307
                     /locus_tag="PK015_00100"
                     /inference="COORDINATES: protein motif:HMM:NF021438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HOL32905.1"
                     /translation="MKKLILSLTAVAMFLPLMLVAQVYSIEGYWHDPDKTAKVQIYQP
                     YTDYYCGKIIWLKEPMENGKPRMDIKNPDPNLRSRPLMDLVIIKGLVPAGENKYENGT
                     FYDYATGNTYSAKVELTGPNTMKLRKYIGVSIMGHTETWTRAR"
     gene            23362..24021
                     /locus_tag="PK015_00105"
     CDS             23362..24021
                     /locus_tag="PK015_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL32906.1"
                     /translation="MVHKTDKYLLTDVNHLRRIKFAQFRSQIPALIILVLAIIMNSIF
                     PNNNRLYYDVALIIAILAIAAYIIIGLCYRYRQRIPIPPENAIVSPVQGKIAYIRRID
                     DNTLINIRKSLFDRVEFRSPHSSAYLENGVVQFETPAGKAYIRLNFKKPYWFSEPDFT
                     TGNLIGMVTGSGSCTISLPGKVSLNFQAGDNIDAGEPLWEDINSLNYDSSSSPYERVN
                     L"
     gene            24105..24878
                     /locus_tag="PK015_00110"
     CDS             24105..24878
                     /locus_tag="PK015_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL32907.1"
                     /translation="MLSYLVEEAEINEASGIASSLKYPGLLYTHNDSGGEAAVYVLNQ
                     IGLMPAKIILKGIENRDWEDIATCYDPKDKKPYVYVGDIGDNNSSHSSSFIYRFEEPE
                     IKDTLILVHNIQKIEFVYEDGPRDAEALMVDPKTGDIYIISKRDNPAKIYQLSYPQSF
                     TEVNTARLIGTLSYKWVVAADFSHSGDYILVKTYNQIYRYKRRPNKTVIEALMTKPKI
                     LPYELEEQGEAVAFDAKGKGYFTISEKAENTEVKLYYYR"
     gene            complement(25126..25563)
                     /gene="dtd"
                     /locus_tag="PK015_00115"
     CDS             complement(25126..25563)
                     /gene="dtd"
                     /locus_tag="PK015_00115"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOL32908.1"
                     /translation="MRIIIQRVNEAKVLVEQHIVAHIKNGLLLLVGFGKDDDAAKIPL
                     AKKKVLELRIFHDENNKMNLSVKDIGGSLLLVPQFTLYADCRRGRRPDFTAAAPPVLA
                     ENLFNQFTESIKETGINVQTGVFGAHMEVALVNDGPVTIFMEF"
     gene            complement(25563..26180)
                     /locus_tag="PK015_00120"
     CDS             complement(25563..26180)
                     /locus_tag="PK015_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL32909.1"
                     /translation="MKKRLFFGFLLIGMALTLTSCFVHSSLMYFEKDTEGLPNLLSNP
                     GFNAYSLDPRESLKGWSVLIEPADQEQTPPNIVFIDGERALHGSSSLRIDASPYKVSI
                     ISDPFKVNRYGGYYVRCYTCSSEPKGPQINLRMLTFKADGSIDKKFKDNIQSKNEWDK
                     MSISAGFLSPGVSFGRVIIEIPPFTQGSVWLDDAGCWEVHHFKID"
     gene            complement(26177..27394)
                     /gene="rodA"
                     /locus_tag="PK015_00125"
     CDS             complement(26177..27394)
                     /gene="rodA"
                     /locus_tag="PK015_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOL32910.1"
                     /translation="MINKKKFDFVLAGYLIALIALGCIVIFSASTTTIGGYTNTQNYW
                     WKQAIFAVISLLAVYLLLKIPMSIFELLIVPAYILNILALILVLFTPPVNGAHRWFSF
                     LGINYQPSESAKLLTILVVARAISQDNMTEIKQIFIALGLTVLPALLILIEPDFGSTL
                     VFFFSLLAMLIAAELPLYYILLLISPVISIITSFWWIAIIIWIIVLVYLLLRARLSWI
                     TISIASILNVFLALITPVFWNGLKDYQQQRILSFLNPMADPLGSGYQIIQAKIAVGSG
                     SLFGKGWLNGTQKNMHFLPEHHTDFIFSVIGEEFGFIGSLVLIGLFFLFFYRFINDIG
                     EIRVRERRIAAAGIFAYLLFQTFINIGMNIGLIPATGIPLPFISYGGSNLLFSTLAVG
                     VVLKYLNERGLMK"
     gene            complement(27407..29314)
                     /gene="mrdA"
                     /locus_tag="PK015_00130"
     CDS             complement(27407..29314)
                     /gene="mrdA"
                     /locus_tag="PK015_00130"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOL32911.1"
                     /translation="MKNYYLHIFRGVLIILCMILVVMLFKLQVVKGSYYQRIAESNFV
                     RIRRIPATRGEIYDSKYRPIVTNIPSYNLYLTSGKIADMNALSSFLAYNFNIDAEELR
                     KMVFEQRFKTYEEILILDNIPYETVIKLSEKVSDFPELSFRSGTTRNYLYPNHFCGYV
                     GRIDAKEYERYKDEDYSLNAYIGKSGLERYYEVLLRGKDGKEIIQVDAQGRSLELFKD
                     NIFLPPQNGLSLILTIDNELQSYANEIFPQGAKGCIIVTDVKTGGILAYISKPDYDPN
                     LFMQKITPEVWASLNTPAKPLLDRVINATYPPGSVFKPVMGTLGLEKGVIDRNTHLAA
                     CRGGLQIGNRFFRCWFAAGHGSLSIVDAFKVSCDVFFYDLINHMELDEVYAHTRACGL
                     TDKTGIDLPNERSGFFPDTKYYKKRIGRVTGLSGYKANLAIGQGEVLCTPMEINTFYA
                     AIARGGLWLQPHLLNRTMGTARINREQIEPLKSYRMPWSANTQQIIKEGLWAVTNAPG
                     GTARAINVPGATTYGKTGSAENYMGKLTHAWFTGFIETDKPGIVVTVFLENAGGGGAV
                     AAPIANKIFNYYMGNIERIERTAPIPPQFLTAEEQEAEFGMEMETSSTTENYVPSPEL
                     PLTENHPESNP"
     gene            complement(29316..29810)
                     /gene="mreD"
                     /locus_tag="PK015_00135"
     CDS             complement(29316..29810)
                     /gene="mreD"
                     /locus_tag="PK015_00135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOL32912.1"
                     /translation="MFWKHIWTFIFGLIIFYLQVLIMPALEIAGVIPLIFIPWIVYII
                     WTRDYNVSLIIIFLIGLLYDTTIPETFGMYTFIFIIMGIVIDLFRKPFESKSVVAQIL
                     SLLVANIVFHLISYFIWGISYSFTSSLISTIILAFFYNLAMSMVVYWTIYLISHLRIE
                     LKHD"
     gene            complement(29812..30504)
                     /locus_tag="PK015_00140"
     CDS             complement(29812..30504)
                     /locus_tag="PK015_00140"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HOL32913.1"
                     /translation="MNKNKNISPIINYNGKRIERIPVQTRILTSEDNIAQVIREYALP
                     LMQQGDILTISESPLAITQGRAVPVENIKPSRLARFLSSKVMRVPYGIGLGSPTSMQC
                     AIDECGALRIFCAAVVGGFTKKFFKRSGDFYRLAGKQAALIDAAETTTIEPYIHTVIK
                     GPIHPEKVAQELSTELGYPIAIMDINDIGGCWVIGASKGISKQFLEQVMKDNPQGQSD
                     ELTPFCIIRRID"
     gene            complement(30501..31259)
                     /gene="mreC"
                     /locus_tag="PK015_00145"
     CDS             complement(30501..31259)
                     /gene="mreC"
                     /locus_tag="PK015_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOL32914.1"
                     /translation="MKRFVLPIILVVLSLIMMMGSTETRIHRASFFSKTIFFPISSSL
                     ESIKTNATLKKEVQQLSKQLTETKLENLKLRNIIMESQTRESITFDTGQVELVLAEVI
                     GYSGPFQQRNLIVNKGSLNGIKPELPVISTNGIVGKVVTVASDHSIVLPFTNPRFQLP
                     VMDKDTGVQGILQADINGKIYMNMIKIGSQISVGDTIVTSNLSDLYPKSYPVGIVSRI
                     HESKDYLFISAEIQPFTQVENLEHIFIIKKNNIL"
     gene            complement(31338..32351)
                     /locus_tag="PK015_00150"
     CDS             complement(31338..32351)
                     /locus_tag="PK015_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOL32915.1"
                     /translation="MKANDIAIDLGTANTLVYKKNAGVVINEPSVVAVSSDNKRIIAI
                     GQQAKVMLGKNPDEVKVIKPMKDGVIADFQVTELMLRDLILRAQKKRLLVRPRVIVCV
                     PSGITEVEKRAVRDSALHAGAREVYLVSEPVAAAIGADLPIEEAFGNMIMDIGGGTTE
                     IALISLSHIVVHNSIRVGGDKMDNDIINYLRKKNNLHVGLLTAEKIKTTIGSAYPLKE
                     ELTMEVRGRDIVSGFPVTIKISSEEIREALSETVSTIVDSIKRLFERTAPELAADIAE
                     RGIYLTGGGALLKGLDEKISKTVDLPVYVVPDALECVVKGAGKVLDDIDHYREVLIKR
                     IED"
     gene            complement(32627..33982)
                     /locus_tag="PK015_00155"
     CDS             complement(32627..33982)
                     /locus_tag="PK015_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOL32916.1"
                     /translation="MLTFEYRNLLHSELIPTAIPESRIRDYARACKLANEEIQADRQA
                     GILGFYDLPERDVSHILNFMESLDPKIDTMVVLGIGGSALGNKALYSALKTERNLDKK
                     LFVYDNVDPVFLFEILNQINLDTTIFNVISKSGTTAETMAAYLIITDILKKRYPEDYK
                     NRLIITTDKDKGFLRKLCNEEHYPSFVVPDNVGGRFSVLSDVGLVSSAFAKIDIQKLL
                     QGAASMRNRCADLTNIYDNPAYLNGLLHYLYFREGKNIAVMMPYSNSLYDLADWYRQL
                     WAESLGKRYDLKGREILVGQTPVKALGATDQHSQIQLYTEGPYDKVITFLTVLNFQHD
                     YVIPNLHPESEDVSYLGEHKLSELLNAERLATEIALTKAKRPNANIIFPQIDEFHLGE
                     FIMMYEIQTVFTGKLLHINPLDQPGVEAGKKATYALMGKPGYDKEREEIQQYLQKLGK
                     K"
     gene            complement(33993..35714)
                     /gene="ptsP"
                     /locus_tag="PK015_00160"
     CDS             complement(33993..35714)
                     /gene="ptsP"
                     /locus_tag="PK015_00160"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOL32917.1"
                     /translation="MHIIEGLVIYPGLIMGRARKLEPISWDLPSTHIAASSLTTEIQS
                     FEEAVSSLAKTIKLQLEELKDSEEEQAILHSHLLILQDPELVSQVKDYISQNLVPAPF
                     AIQQVFSQVQNHIKNLSNDFLAQREADYRDVEHRLLLEITGQQQEVLHNWAPDQIAIL
                     HEASPSLVSAFAYYKVPAYCSEQGSLTSHASILSRALKIISITALPELWKKVSENEKI
                     ILDGLSGKVIIEPDSATLEYYEKLFANYPKPEEPVILEKDSPIKTKDGRRIKLRCNLD
                     PLSDLNEPSKIAADGVGLYRTEFLYLGKDELPSEDYQFSIYKQVAEIIAPHSVIIRTF
                     DLGGDKLSHLIPSNPEDNPYLGSRGIRFSLSHKDIFKTQIRAVLRAAIFGKVKLMFPM
                     VKDYRDFLQAKEIVEECKIELAKENIPFSKNLPLGVMIEIPSAALCAEELAENCDFMS
                     IGTNDLVQYTLAADRNNSALSDYYITHHPSVLKLMMLTLQAGNKYEKPVSICGEMASQ
                     PQYVPLLIGMGFKELSVGTAAFLRCKNIIHRCDENLFNLIKNTDLEHLDSIENLIFEQ
                     LKPYYKF"
     gene            complement(35719..35985)
                     /locus_tag="PK015_00165"
     CDS             complement(35719..35985)
                     /locus_tag="PK015_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOL32918.1"
                     /translation="MSRRKVKVVNKLGLHARPAALLVKAATKYRSDFFIEKDGMRVNG
                     KSIMGVMMLAAECGSVLDLIADGVDEEYLLDEISELIASGFGEE"
     gene            complement(35990..36943)
                     /gene="hprK"
                     /locus_tag="PK015_00170"
     CDS             complement(35990..36943)
                     /gene="hprK"
                     /locus_tag="PK015_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HOL32919.1"
                     /translation="MKVITIRELFETKKKDLALSLVTEPETLNKKLSSQFINRPGLAL
                     AGYLDVFFSDCLQVFGEVEVRYLQTLPEELMLERMRRIFQMDIPCIIITKGLTFPPSI
                     EYLANDLNIPILSSRLSTAQLIQLLNRYLLDVFALEKTIHATLVEVFSLGILLTGKSG
                     IGKSECALDLIHRGHSLVGDDLITIRLIDDKLIGKSSRDLGSFMEIRGVGFVNVERMF
                     GIERVRKQKEIDLQIELMPWQENIDYERIGLANNYAEHLGVKLPIIYLPVSPGKNVSV
                     IVEVAAMNMILKGVGYDAAEDFNRLLHEQIRKQTLQKPIVT"
     gene            complement(37008..37547)
                     /gene="raiA"
                     /locus_tag="PK015_00175"
     CDS             complement(37008..37547)
                     /gene="raiA"
                     /locus_tag="PK015_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOL32920.1"
                     /translation="MQITITARHFELTKAIRDYVESSCQKLNRYFDHIINIHATLSLE
                     DNRNICELSLHAPRYVLKSQAEEMDMYLAIDSAVDKLEVQIKKLKDRITDHQKKKPLK
                     EQFISYSHESDIIVNPENHTRKTIKTKRTVAETLSLQEALDKLDRQKENFLIFRNVET
                     DKLNVLVKKDEQNYKLIEP"
     gene            complement(37564..38964)
                     /gene="lpdA"
                     /locus_tag="PK015_00180"
     CDS             complement(37564..38964)
                     /gene="lpdA"
                     /locus_tag="PK015_00180"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOL32921.1"
                     /translation="MSEYKVTIIGGGPGGYETAIRLNQYGIECLLIEEERVGGVCLNR
                     GCIPTKALVKSAELYYEMSSASNYGLTSYKPELDYKQVFNRKNAIVEQLVNGIELIFR
                     KRNIPILKDRAIAISLENDKYLIQTAKGEQIETEYIVIATGSLPKSLPNITIDEVNIL
                     SSTGMLNLSTLPESLAIVGGGVIGCEFASIMNSFGVQTSIIEFLPRILPSEDEEISKR
                     LTVALKKVGIKIYTSTAVENIKEIEQRQKLSLSNGSTLTVDKVLLSVGRIPNCNLDWN
                     QMELETEHNAIVIDEFMRTNLNHIYAIGDVTGKLALAHTASKQGEIAAAHIASNILNI
                     PYHHPPLNYTYIPRCTFTSPEVASVGHTEEEARKKFGELKIGKFPYSANGKAMAMSST
                     YGMVKTIARADNSELVGMHIIGANATELIAQGAILISLGAKSEVIDYLTFAHPTLSET
                     IKESLEDLNNLSIHKI"
     gene            complement(38976..40433)
                     /gene="rpoN"
                     /locus_tag="PK015_00185"
     CDS             complement(38976..40433)
                     /gene="rpoN"
                     /locus_tag="PK015_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOL32922.1"
                     /translation="MNSFNQNISMQEKQELALKPKMLQSLKMLTLPILELENYIKQEL
                     VNNPLLELRDEKEEEDLEETPMAFVPEESQKNLDDTDSSFEWGQTLSEAKELTEILDQ
                     WNEYHQNYEGSSSKPEKDKGEVLLIYEESGIEKFMQQLYPLSLPDNEIDFIAEMLDNC
                     DSYGYLSPEFNLEKTARDYNISPERANELHNIVLQLPPKGITARNISECLLAQLSEEE
                     RQNSILVGLCTDQFENLIHRRFQKIASYYGVSEDCILFWRDVVAKLDPKPGLRILTPN
                     TAYIVPDITIKRIDDDFEVIINDHISPNIIISPHYLKLMAHGFLDKETLKFVRERINS
                     AKFLIKSIYMRTRTLERVTKAIIKYQSDFFYNGTGIIQPLTYSVIARELSVNESTISR
                     VVKHKYAETPYGIYALKDFFSSTAGIDTRNENISRQQIKSFICRMVENEDKKHPLSDQ
                     ELVNLLKKEGLIISRRIVAKYRFELGILNSRLRRM"
     gene            complement(40430..41179)
                     /gene="lptB"
                     /locus_tag="PK015_00190"
     CDS             complement(40430..41179)
                     /gene="lptB"
                     /locus_tag="PK015_00190"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOL32923.1"
                     /translation="MEIHKIQAQHLAKIYGKRKVVNDLSMEMQQGEVVGILGPNGAGK
                     TTTFYMILGLTKPNEGKVLLDNKDITHLPMYRRARLGLGYLAQTPSIFSKLTVEENIL
                     AILQTLKISKQEQKRKLEEALAELNLTPLAKQKAYTLSGGERRKLEITRSLLTNPTFI
                     FMDEPFAGVDPITVADIQDIIAKLRDKNIGIMVTDHNVFETLKIVNRAYIIFEGKIIV
                     SGSSWELINDEKAKKLYLGDRFLTNPFESRQ"
     gene            complement(41212..42294)
                     /locus_tag="PK015_00195"
     CDS             complement(41212..42294)
                     /locus_tag="PK015_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL32924.1"
                     /translation="MKKKNFLIGSVLLLILLPIWAQKSEPLRVIHSDKLYLTKVQEEQ
                     VMRLEGKVHFFYGKTEFHSDRATIFNLQKIARLDGNVKVNNDSLTLFADSLTYYRIPD
                     ELNAGGKIYIEENQKGSAFRWFRSNYAIYNQKYNQITVWENVSAYDKEENVSIFCGYA
                     FWDRNEGYAYMIEKPRIITAQEDTLYVNADKMEYFEKERKLVATFNVQAQTRDYRTVS
                     DFLLYFLKEDKAVFTGMPEFHSDYAKAEAGEFYLYFKERKLNRAELVDSCRVEFSEEA
                     MGPKFNWVVADYIDIGFEENQIRSFNAERAVQYRYIRQKTDKNDYFDNTATGDYLEVQ
                     FTPENKLDYMKMNMNIKGIYKFQNKS"
     gene            complement(42266..42838)
                     /gene="lptC"
                     /locus_tag="PK015_00200"
     CDS             complement(42266..42838)
                     /gene="lptC"
                     /locus_tag="PK015_00200"
                     /inference="COORDINATES: protein motif:HMM:NF018537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOL32925.1"
                     /translation="MSKHCSPILILSVELILLCSLLALSACGKSKLDIQTESMKRGIP
                     DETSTNVTLTEYEQNKIAYILNAERIDRYYDRRILNAYKVEIQALDKKTGGTSFLKAD
                     STIVDEARNMIMAHGNVKLISAEGSIYTNRMIWDRNSDVIVAPDTVMLVRAGNILRGR
                     NLRTNLSIYPTEMDSISAEGLFEEKEFLNW"
     gene            complement(42798..43355)
                     /locus_tag="PK015_00205"
     CDS             complement(42798..43355)
                     /locus_tag="PK015_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HOL32926.1"
                     /translation="MNFINIQKPLKPSLAWTKIKLMVFDCDGVLTDGRIIYDSAGNES
                     KNFDAHDGMGFNILKHTDIKTAVITGRNSPLLERRCQDLKIDFLFQGVLKKLDKINQL
                     LQELGLDFDNLLMMGDDFNDLPLMLRSAISVCPADTCEDVKAFCDIITQHNGGRGAVR
                     ECIELLLTNQGIYEQALLSYLNSIS"
     gene            complement(43352..44155)
                     /gene="kdsA"
                     /locus_tag="PK015_00210"
     CDS             complement(43352..44155)
                     /gene="kdsA"
                     /locus_tag="PK015_00210"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HOL32927.1"
                     /translation="MDLYQKLKTTPAFFLIAGPCVVENDTVMHKVAETLVQLQEETGI
                     IVVFKASYKKGNRSSVDSYMGPGEEEGLRSLEHIKSEFHLPVLTDVHERIEVEAAAQI
                     CDILQIPAFLCRQTELIQATAATGKIINIKKGQFMAPEDMQQTALKAGNNQQILLTER
                     GTCFGYHNLVVDFRSFSIMHSLGYPVVYDVTHSLQLPSAGKVSGGNPEFAPMMAKAAI
                     ATGKVQGLFIETHPEPEQALSDAASMLRLNMLAPLIKECLKIAHLKDNE"
     gene            complement(44164..45336)
                     /gene="metK"
                     /locus_tag="PK015_00215"
     CDS             complement(44164..45336)
                     /gene="metK"
                     /locus_tag="PK015_00215"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOL32928.1"
                     /translation="MTALHENKPCEKNEYVFTSESVSEGHPDKVCDQISDAILDAYLT
                     EHPSSRVACETLCTENRVILSGEISSPGNLNLDIEPIVRNVIRNIGYIHPGIGFDYNC
                     EINNYLHSQEAALRENEGAGDQGLMFGYACTQTRHYMPAPIAMAHKLLQNLAKLRKNG
                     TIPYLLPDAKSQVSMRFCGRKPVSVETVVISTHHKHISKSEFEEMKRAINQLVIKPTI
                     EEMENDTCSTFQANNYQVKINPLNEWEDGGPAADTGLTGRKIIVDTYGGWAQHGGGAF
                     SGKDATKVDRSASYMARHIAKSIVASGLAEECLLQFSFIIGEAEPVSLMVDTFGTGKM
                     SEKELESLIRKSFPLTVKGIIEYLDLRKPIFFPTASYGHFGRDDENFTWERVKELK"
     gene            complement(45506..45581)
                     /locus_tag="PK015_00220"
     tRNA            complement(45506..45581)
                     /locus_tag="PK015_00220"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45546..45548),aa:Lys,seq:ctt)
     assembly_gap    45740..45839
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            46322..47398
                     /locus_tag="PK015_00225"
     CDS             46322..47398
                     /locus_tag="PK015_00225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOL32929.1"
                     /translation="MEEKEKKGFFQTIWLLIIGIFKTAKYDIAVFWKYISNSQWRKRL
                     WADLTNFVKSFYQILMKERVLREAGALTYITIMGFVPVVVFLIFLAPTLPFLDLKEKI
                     FSLISENLIPSAALQVQNIIQNMLENKAGFNIINFIVIAVSSYSLFIVIRDSFDRILR
                     HEFRTKPGLISQIIKFLGTLILGLLIMIVLLSTSSIPIISGILKLPVFKWFSFLIPFL
                     MQYIVLLLLYSLMPSVTVKATSLVRGAFWAAIIWLIARSGFDFYIYNLTTYPERYGAV
                     SALPIFLLWIYVNWVIIMSGMVMVSVYENRKNGKEPTESPQQMVRVTVEMYTNSKLNK
                     RIEKLLAPEELKNIVQQVDNDEDK"
     gene            47395..48942
                     /gene="purH"
                     /locus_tag="PK015_00230"
     CDS             47395..48942
                     /gene="purH"
                     /locus_tag="PK015_00230"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOL32930.1"
                     /translation="MNKYALLSVSDRRNIEKLAAALNELGYAILATKGTSNYLDNIGI
                     NNIEIARLTRFPEILNGRVKTLHPRIFAGILADRENLEHMKTLEELGIDCIDLVAVNL
                     YPFAETWSREGVTHSELIENIDIGGPSLIRAAAKNYKSVMVLTDPQDYESVLAYLQNN
                     KEVPLELRIHLAHKAFEYVSHYDTIIAEYFKSLDSFKENSPNIPSYYSISCKLEQPLR
                     YGENPHQMAGFYLSHQSPLEVLSGKELSFNNHQDIDATLRGLRLFEQPAVIITKHCNP
                     CGIGVDSDLALAYEKAYKSDTDSPYGGIVGVNRSLDIKTAELINRVFTEIIIAPDYET
                     GVLEFLKKKKNRRVIKYNPELLNKFSEPWELKCLTYGWLLQSWDLVQENPEEWKVVSE
                     QKPEKDEWEALIFAWKVVSLLRSNAIAITGKDQVYGLGAGQTSRIDSTHLALWKAQRY
                     GHNLKGAVCASDGFFPFRDSVDELHKYGIKAIIEPGGSKSDNEVIEACNEYGITLVFT
                     GYRHFKH"
     gene            48991..49085
                     /locus_tag="PK015_00235"
     tRNA            48991..49085
                     /locus_tag="PK015_00235"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:49026..49028,aa:Ser,seq:gga)
     gene            49138..50844
                     /gene="sppA"
                     /locus_tag="PK015_00240"
     CDS             49138..50844
                     /gene="sppA"
                     /locus_tag="PK015_00240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOL32931.1"
                     /translation="MKKNSGIYIGCGVIVLILAVAFLAGYFIGFAPQGKNVAQGSWLV
                     LDLSGQVTDYSILQSSNFLGYNPLSVTDICYRIRKAADDNKIKGILIKPNLLQTNYAN
                     LNEIDAALQDFKKSHKPVIAHGEVLAQKDYLLCAMADKIFMDPSASAGIVLEGVASNV
                     LFYKDALKKLGIKMHVMQEGEFKGAAEPYTQNSLSPGTEENLRKVLKSRYELIQQDIS
                     KMRSMKIDTVQNIYENRPDLTIDAQEAIKYGLIDEAATWEELQKKYDITDKYQISISD
                     YKSGTEASISSDKIAVVYLSGNITPSINYNNPNVISLNSIDRILDKLADDKTVKAVVL
                     RINSPGGSALESELIYQRIKAFEKPVVVSMGGMAASGGYYISCAGDYIIADPQTITGS
                     IGVMMALPEAEELGNKIGIESQTISYGKFAGAGNILEKTDPELLASLKRESASVYAEF
                     KQRVMDSRKIGADKIDLVAEGRVFSASDAKGNGLIDDIGNLQFAITKAADLAKITKYG
                     VESYPKKVTIWDLFRQGNWLQTSSAKAINKESNAIEPIEDYLKKTLPTNQWLYFCPYK
                     LD"
     gene            50851..52407
                     /locus_tag="PK015_00245"
     CDS             50851..52407
                     /locus_tag="PK015_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOL32932.1"
                     /translation="MNQSRSHKTEHSYSKRDKYITEIIVNVHPLEKRVAVLEDGRLVE
                     LFVERKDKQNIVGNIYKGIVKDVLPGMGAAFIDIGLERTAFLHYSDIVMDFLDIYENE
                     TPHPRLNPKDSSQIGNLLKPGQEIVVQVHKGPLGSKGARLTGQISVPGKYLVLFPNKH
                     KIAISRKIYNPNERYRIRNIISNFKDPNFGLIVRTEAEGCEEDELRTEYEALLKTWKL
                     IEKKIRYHKAPVCVFEENALENYLIRDLFGENVDRLVIDDKAFAKSIIHQMEDISPEL
                     IHNIEIYKEDTPIFDAWGIEKKIETIFHSRIYLPSGGNIKIEQTEALVAVDINTGSFT
                     GKTNYEETIKKTNLEAAAEIARQIRLRDLSGVIIIDFIDMANEQNKQEVLEMLRKNLR
                     RDRAKNKVYNFTELGLVEMTRKRMRTSLIANFSDPCPLCHGSGRIVSKDTMLMRIYRW
                     LNRSEYFIKDRKLRIIVHPELLEHIQNNYEYFTAYKDQIEIVSDPEMRTDQFKVLLLP
                     DLEDITAKFS"
     gene            complement(52430..56602)
                     /locus_tag="PK015_00250"
     CDS             complement(52430..56602)
                     /locus_tag="PK015_00250"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOL32933.1"
                     /translation="MRKCYIPFLFFFLISLTVLSALEYTPGQLIFKTSQPLNIKSDQT
                     GITSFDNWLSTLGAHNLRPIRGMPNNQYFLANLEQEPNWQDLKEGKIKFAGIEYIQPN
                     YLSTFHTIPNDPLFPRQYHTIISNPQAWDYCTGSSTVVVGIVDSGCLINHPDLAANIW
                     VNTREIPDNGIDDDNNGYIDDIYGWDFVDAPELADIAIGDYLNPDNNVEDENFHGTAV
                     AGIIGAVGNNGVGICGVNWNIKLMIVRSGFLTVDGQGYLQDDDAAAALIYAADNGCNV
                     INMSWGDPNYSPIIGDACAYAYSRGVTLVASAGNDPGPYISYPAKLSTAISVGAINRT
                     RTLAGFSSYGPDLDLVAPGEMVLSTYKLDPGNQYYEQSGTSMSAPFVSGAAALLLSLH
                     PGLSPSEVRSRLLTATDDLGTPGFDQYYGHGLLNTKKLLENTAPPLVYIDSPLDQSGL
                     TESFDIIGTVQGNNFFRYSVMYSNKSVPSLLDWYDVETHHNYPKFHTQPVQNGVLAHF
                     YIPESFAEGEYIIRVQYEAINGSKYNFFRTVIYDPSPPQMKENSLQQFTRYDKQNLRY
                     YIIAVFNEPVRSELDIIASNGTHYHSYGAMLDTLQIWSLPETLPEGQIDIQVKATNVS
                     NLTFLSDWYNGFLNIHYELIPNYGYIYSPLGGPKVPLNYSYDYNGDGIKEYIAMDLPK
                     TGYGPVYAYQPEAGGHIVSHSFGDSFWLLGAGNTNNIGQELLTLKYGTATVLESQNSS
                     YPNIALWEDRSIDGGTIADYSNDGIDDLLLVKTLPTERVIQAYKRSGNIFVPKNTLHN
                     TSSTTSYNAFVPTIIVKNFDNDSYPDILVADTDGDVMIYEIRNDNFAELTWTQKMPVG
                     NTYYLTSGDFDGNGRQDFFIGGYYTDKLDPNLNFWYFEGFKNVSNNNYTSMGSIMFNQ
                     VASKNAIQAADLDNDGKDEIILAISPNLYIVKYQDDKFQPQFYGNSYNTYSILAYIDH
                     PNQSFFLTNYEVEKDSLISVVWTQDAPYTGPPTPINFIARPLNESSVQLSWIDNGADY
                     YRIYRKEENNQIYILDNVTGNSYIDLNVEEGRTYQYAITAYNSNFTPSESAPTIWQSV
                     IPYPVPHIESVTMIGPNRLRVMFDQPMATSICNPSLYTVDKGMSHPSSVNYINYYYGV
                     QLQFSQIFPETSEPFLLTLGTMLSAYNIPLAENQYEFYWIADTEPPKVTGAVVLNDQK
                     TVEITFSESLAPDNPSPEQLHNYTLICHSNDPDNSIVSVSHLDDRVQVSLSHKLKFGS
                     SPYQIVVRNVRDLAGNPIIPQYSTTRFYMSNNHDLKNLVVYPNPVRFNDLQCHILNFP
                     AGKRGHIKIYDSSGNLVRSSAIGPFNPEINNIDWIWDLKNNDGKLVSSGIYFYVVEMG
                     GNIARGKIAIIK"
     gene            complement(56614..57537)
                     /locus_tag="PK015_00255"
     CDS             complement(56614..57537)
                     /locus_tag="PK015_00255"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOL32934.1"
                     /translation="MKEFSVHKVALILSLIMLFSVFLNADIHHNAGKYGYQFLDISTN
                     PISLSLAGRGISSSSELASFLRQPASSTIEAHKSIGASHIIWLADTSCDNLYYSWSNR
                     KSHFGLAFRSLDYGELELRDDNGMLIGHYSPLDIDLLGNYAKRLTPSIYAGVNVGIAY
                     EKLDTDSSLGLHTDLGVTWLTPIAGTQFNLSVRNLGISSAMDEERTLFAPSLEMDLSK
                     SINYQNTDITFELSGIKAVDENWKAAVSSEIELFQRLLALRIGYKINYDAENLTAGLG
                     LNWKNLSIDYGWGSFSDHLNNVHSFGLSYHF"
     gene            complement(57534..60641)
                     /locus_tag="PK015_00260"
     CDS             complement(57534..60641)
                     /locus_tag="PK015_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOL32935.1"
                     /translation="MHFQPIRIICLVLVITLSVYVEAMVPPHPLYKDIPAAYTPTKIE
                     LPTNFTSPNKLAFRTLPHNILVLRAQFQDVHFINNPTWPDNLTHDDAFFDRWMLHLSD
                     FYADASHGNYELNYTLHPSVFTLPENMSYYGRDTADNIDVNLNEFTQTLLQMADPEID
                     FSLYDGLIIFHAGAGQESDINGIRTGEIWSTFLTRKRLQSWFDPDNDNYQGWLADGVY
                     LKNIVIVPENEYQDYFPAEGQPNAESYIFSIYGVLAHQFGHILGLPSLFDTDSSNGAS
                     QGIGNWGLMGTGIWNANGYVPAQLDPWCRLYLDWEPVITITTDTENVPVDYFLNHNPS
                     ANRLYKLPISSREYFLIENRQQNPDGSTDPYTGLPSYSFPLLPEGEQDYYENYPLLPY
                     FNFMKNRYKGSEWDFMLPGLGGPIPSGMNIPVDGSGLLIWHIDENVIAQNFTPNFDLN
                     SVNANSAHKGVDLEEADGIEHLDTANYSIYKYGSPYDSFRNGNNDYFGNRFHNGLLSL
                     PAAESYYGGVPLEVYDISISGNVMYFSVSFGWKLYANYEGDNPINACMVDFDGDSETE
                     IFYPMPNGLLYMWKNETLMPGFPLALQPVTQNYVWDGSSFYLPMQYQSLARLYKLSNS
                     DKRFIGNFPGCNWATHPVSDGDKLYFALNDNDNTKSYIFSYNKIEDELQQMTQFDFPV
                     VANMILFRNNLYVLNKKGSSYSIWNYDLINTEWEEKQLNLPVDSTLVAIFKAPLQPKS
                     INRAGELIVQFTNSIYVFDNELNLLPGFPYIHNMHTTAPLTIADWDINGSLDLIITSD
                     EGFAIIDHSGSRMSPTSVFLPASDSLAFNSGAVVLDLDNDGKNELLGSFSNNRLICWE
                     DNFRVKKGFPVSFGNRSRNLPFFGKASDGNIYAWVASDNGTIYRTLLPDAVLENFSSG
                     WLTEYGNLQRHSSRDDSFLTNQFLTTELFVPGEVYIYPNPLKPLFGNKLTLNVMTSQD
                     TPIEIKIFDINGSLVYSQKAYARAYLRNRELIDFPEDRLHNGVYIAVISGYRDTKQIK
                     FAVEK"
     gene            complement(60816..61025)
                     /locus_tag="PK015_00265"
     CDS             complement(60816..61025)
                     /locus_tag="PK015_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL32936.1"
                     /translation="MGKAKSFAAKLAHETSSEGKVICPVCNTELKKLKMVTNKKKGSG
                     SWSPKYEYIRVCKCNENDLLAGKKL"
     gene            complement(61071..61634)
                     /gene="efp"
                     /locus_tag="PK015_00270"
     CDS             complement(61071..61634)
                     /gene="efp"
                     /locus_tag="PK015_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOL32937.1"
                     /translation="MATMADIRNGMIIEFKDDLWEVVEFLHVKPGKGPAFMRSKLKNV
                     RTGKVIENTWRESDTFNEVRVERRKMEYLYRDGDFYIMMDTETYEQIPVDASLVGEQD
                     KLLVENMEVSLLLTPSGKILGIELPVTVIQTITECEPNVKGNTASGSGKIAYTETGLR
                     LTVPFFIEAGDKVKIDTRTLSYIERAN"
     gene            62085..63206
                     /locus_tag="PK015_00275"
     CDS             62085..63206
                     /locus_tag="PK015_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL32938.1"
                     /translation="MSITLKTVQDKKDLKRFVYLPEKLNAHRPEWVPPIYSDDMRVLD
                     PKRNPAHRYCDSVLLLAYDGEELIGRIAGIINHRYNDYANTKTARFSFFEAPDRLEVS
                     KTMLDYVENWARDKGMQKIVGPQGFTEEDSEGFIYEGYNETPTLGCIQNLPYVNEHME
                     KLGYGKEVDWFVYKVEVKKAMTETYARLFERASRNPNFHLLEFTKKKQLKPYVRPIFD
                     LMNEAFMVLYGYTPLDEAEIQSMADRYMPLLDPRFIKAVETTDKQIIGFIVSIPNMSE
                     GIKKAKGRIIPFGIFYMMQAAKKSKQLDNYLGAVKEEYRGKGVDVLLGYSQLKSAYEA
                     GFEIIDSHHVLEDNIKMRAENERVGGVIYKKYRLYSKQL"
     gene            63472..64812
                     /gene="der"
                     /locus_tag="PK015_00280"
     CDS             63472..64812
                     /gene="der"
                     /locus_tag="PK015_00280"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOL32939.1"
                     /translation="MRKYIVSIVGRPNVGKSTLFNSLCRKRSAIVDAEVGVTRDRKYE
                     DVEWNGKVFKLVDTGGIVFDDKEELTKKIRHQALLAIDESDLILFLVDAQTGTTDIDK
                     AIAKLLYPHRDKVMLVANKVDNEKYEWELYDFLQLGLGEPFPVAAIKGRNIGNFLDEV
                     LKHIPETQSLSETQESGPTRIAVVGKPNVGKSSIVNLLLGKERQIVTDIPGTTRDSVD
                     SYFRYYGKEYVLVDTAGLRRKTKVDYGVEYFSVMRAIEAVDRSDVVVLVLAADEEISE
                     QEVKIASYARRKMKEIIVVFNKWDLVEKDNKTTGKYVAELYRKMPFLQFAPVLFISAK
                     TGQRINRIMETVVKIEQESEKRIPTSELNRFIETVVEKYPPTHRSGKEVKIYYVTQAG
                     VKPPTFVFFCKRPALVTENYRRYLNNQIREMFSFEGVSIKLIFKGKTEDERMAE"
     gene            64809..65465
                     /gene="plsY"
                     /locus_tag="PK015_00285"
     CDS             64809..65465
                     /gene="plsY"
                     /locus_tag="PK015_00285"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOL32940.1"
                     /translation="MNLTLLWIILCICAYLIGSIPFGYIIGMIFHHQDIRKGGSGNIG
                     ATNALRLYGALSGIAVLILDFLKGFIVAWLLYKVVPDKFGYLIGDKPLNAFIFNLPAL
                     MVILGHMYSVFLGFKGGKGVSTALGVFIYIATIPMLLSLIVFLIVVAITRYVSLGSIV
                     TAFSFFIIDFLWNILVIKDYTFPWFQLTVVLLIIYKHYPNILRLLEHQETKISFKKGQ
                     "
     gene            65469..66839
                     /gene="purF"
                     /locus_tag="PK015_00290"
     CDS             65469..66839
                     /gene="purF"
                     /locus_tag="PK015_00290"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOL32941.1"
                     /translation="MCGIIGIYGNPEAARMAALGMFAEQHRGQESCGLAVSDGKVIHL
                     YKSMGLVKEVFTDEVLKTLPGYIAVGHVRYPTKGAATKFNTQPHLVETLFGPSYALAS
                     NGDLVNYDQIRARLEAENVYFNSDNDGELLVKYLAWQIIAKNKDVVEAIRCLMKDIKG
                     AYSCVFCTRKELYMFRDPLSIRPMIWGRNINGDVVVASESCVLDILDVNGRSEVPAAG
                     IIEVNADGVKVIENNPAQFRQQNHNSYCVFEHIYFSRPDSYHFGENVFEVREDIGRKL
                     AEQDKGLEADCVVPVPDSANFMAVGYSAASGIPLSLGLIRNHYIGRTFIKPEQTIRDE
                     SVRQKFNVLPNFFKGKKVVLVDDSIVRGTTIRKIVRMIKLAGAEEIHLRIGSPAICYS
                     CYYGIDTPHREELAANRYSLEEMKERIGVNSLKYLDMNSLRECLKEPDEYCYACFDGK
                     YPLEKE"
     gene            66836..67801
                     /gene="rfaE1"
                     /locus_tag="PK015_00295"
     CDS             66836..67801
                     /gene="rfaE1"
                     /locus_tag="PK015_00295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HOL32942.1"
                     /translation="MIAEIIKNFQGKRVLVLGDVMLDHYVWGEVERISPEAPVPVLEV
                     QKEEFRLGGAANVALNIKTLGGEPILLGVIGKDTAANDLIRLLTEKDIDTDKLVIDSS
                     RSTTLKTRIGAINQQIVRIDYETKTDINLETMRKLLTNLEGLISNCDAMIIEDYNKGL
                     LTEKVIEEALRYAKEKNIPVAVDPKQKNFMAYKGVDIFKPNYKELQTISGKIFENEEE
                     FIQTARDFKLRMNIKNLIVTRGALGMYVFEGRGAHIHLPTCAREVYDVSGAGDTVISV
                     LTLAYISGADIYSAADIANHAAGVVCGIKGTASITKEQLLSSYND"
     gene            67794..68309
                     /gene="rfaE2"
                     /locus_tag="PK015_00300"
     CDS             67794..68309
                     /gene="rfaE2"
                     /locus_tag="PK015_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HOL32943.1"
                     /translation="MIKDKIKSWEEAALLCDNFRSQDKKVVFTNGCFDILHSGHIQYL
                     EQAKALGDILVLGLNSDDSVRRIKGAPRPIVSEKDRAFVVAALQCVDLVVIFEQDTPY
                     ELIRLLKPDILVKGGDWKTEDIVGADIVQAYGGKVLSLPYRSGISTTELFRKIAEAFA
                     DFTMKDNGNYE"
     gene            68302..68724
                     /locus_tag="PK015_00305"
     CDS             68302..68724
                     /locus_tag="PK015_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HOL32944.1"
                     /translation="MNNEKGQYPQEDVGTVIEVEGEKVRVEVVRSGGCKTCSMQSFCF
                     GRNTPAVFDVESELELKPGDRVELEIAPASRIISSLLVFGLPILCLFLGYLIGSIWLD
                     ELASIGIAFAATALSYIILKLIDRKFGKKVQVRIGRKL"
     gene            68721..69080
                     /gene="folB"
                     /locus_tag="PK015_00310"
     CDS             68721..69080
                     /gene="folB"
                     /locus_tag="PK015_00310"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOL32945.1"
                     /translation="MIIRLNDMVFYGYHGVNDEERKLGQRFIVNLEMQTHPETDSVIK
                     RLEDTVDYTKVYAEIREIMESRQFLLLENCANIVADKLLADFPLIWKLKLCIQKPSVP
                     IQGILRSVEVEVQRSRE"
     gene            69077..69544
                     /gene="folK"
                     /locus_tag="PK015_00315"
     CDS             69077..69544
                     /gene="folK"
                     /locus_tag="PK015_00315"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOL32946.1"
                     /translation="MIAYLCLGSNLNNPETQVQQALQKLREDPNIRILKSSTLIQTKP
                     VGNLNQPDFYNQVIEVETSYSSDELLQKALNLENQMGRVRSEHWGPRIIDIDILLYED
                     EIKHTQDLILPHPEMTKRKFVLELLCSIAPDLIHPVLHKSIANLLKDIEKKED"
     gene            69548..70576
                     /locus_tag="PK015_00320"
     CDS             69548..70576
                     /locus_tag="PK015_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOL32947.1"
                     /translation="MSNLSAIILAAGKGTRMKSERAKVTFSFAEKPIIQRVVNTALKA
                     NCSKIFVVVGYQKDSVIACLEEDPRLIFVEQPEQLGTGHAVMMVEPVFQEREDDVFII
                     CGDVPLLKPETLQKLYNEHSSSKASCTILTSIMDDPGSYGRILRNASGEITGIVEFKD
                     ATEEQRAIKEWNTGIYCFQAGDMFDALKQTSSVNNQNEYYLTDVVSVLAKQGKKISSV
                     ILDNPIEVAGVNSQEQLAELEDLFIAEIRKHWLNNGVVIHNPRTVYIGEEVDIETDVE
                     IGQNSIIRGKSELRKGAYIGPNCYIEDTVLCDSSFLEGYNILISAHIPEGHILEFGER
                     TIEDIDYE"
     gene            70569..71072
                     /locus_tag="PK015_00325"
     CDS             70569..71072
                     /locus_tag="PK015_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOL32948.1"
                     /translation="MNKEFLYSPWRLNYIEGEKTDDCILCRYQEMDKDEENLIVYRGK
                     HCYVMLNRYPYNNGHIMLVPYLHLKSLHELPKEVIEDLGSLCQLSEKVLFAAYNCEGI
                     NVGINLGRAAGAGIDEHLHIHMVPRWIGDSNFMSVIGGQRVIPEAFEKSWQRLHQEFI
                     YQLQLKK"
     gene            71083..71523
                     /locus_tag="PK015_00330"
     CDS             71083..71523
                     /locus_tag="PK015_00330"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HOL32949.1"
                     /translation="MTAKRSSNKWFPSKSLLWVLIVVLTGVLITLIYIRFISPKSDFQ
                     DLDEKNLPAIKVIIKNGCGVENLATDYANYIRNKNIEVIRVADTPHPIYNKSLIEMKK
                     EDWQDLKRLQKMTGIQRYTLAVDSDSEAPFIIILGADYDTFMKR"
     gene            71537..71890
                     /gene="rsfS"
                     /locus_tag="PK015_00335"
     CDS             71537..71890
                     /gene="rsfS"
                     /locus_tag="PK015_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOL32950.1"
                     /translation="MADNIKLTDVLNWLNEKQAEHIAVYDVANNCSYTDFIVVCNGSA
                     DLHNKAIANYILLMAKKSKYQILGKAGLEYGQWILVDFGDVIVHIFRQEKREYYDIDQ
                     LFSELTEPIKEEKDL"
     gene            71887..73539
                     /gene="argS"
                     /locus_tag="PK015_00340"
     CDS             71887..73539
                     /gene="argS"
                     /locus_tag="PK015_00340"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOL32951.1"
                     /translation="MIKKQIADNVLEALDNLGLKHTKEFSVEVPNNPDFGDYSTNAAL
                     MLAKENQIAPKQMAEKLAKELRKNKQFKSVEIAGPGFINFRIAPSLYQKVLWDIHRQG
                     KNFGSSDYGKGEKVLIEFVSANPTGPLNIVSARAAAFGDTLYRLMKKVGYEAAREYYV
                     NDAGNQVDILAESLELRLREIQGENIGEFPYEAYHGEYVKHLAHKLNAAEGVRIFVMP
                     EKERTEHLKEFALREILEMQRQSLEKFDVHFDNWVSERTLRQEGVVEEVLSYLTEANC
                     TYEKDDAIWFSSTKFGDDKDRVLMKSDGSITYFVPDLAYHLTKIQRGYTKLIDIFGPD
                     HYGYVPRLKAAFRALNYDENMLEIIFLQQVNLYENGERVKMSKRAGKIVTMDDLISLV
                     GKDAARYFFIARKASAHLNFDLDLALQKNNENPVYYCQYAHARICSILRKARQENIYP
                     NYFKNELIRKLVRPEELDLIKKMADFPELLINIVDKREPHLLANYTHELCALFHHFYN
                     HYRVINPKRIELSQARLLLIETVKHTLEICLDLMGISAPEKM"
     gene            73547..73999
                     /gene="rimI"
                     /locus_tag="PK015_00345"
     CDS             73547..73999
                     /gene="rimI"
                     /locus_tag="PK015_00345"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOL32952.1"
                     /translation="MRVIRLMQDSDLDEVIRIENQVFHPPWPKEAFMEAPCTYSWVIC
                     EDEVLQGYIVYHTVLDEGVILNFAIDPKFQYQGLGTYLLKETLNYLIDNGIRMFFLDV
                     RTSNMVAQDLYKKFDFHPIGKRKNYYSEPIEDAIVMVKYIDERNNENI"
     gene            73974..75584
                     /gene="nadB"
                     /locus_tag="PK015_00350"
     CDS             73974..75584
                     /gene="nadB"
                     /locus_tag="PK015_00350"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOL32953.1"
                     /translation="MNGTTKIYDYLVIGSGIAGLTYALQVSRWGKVAIITKKSLFDCN
                     TDYAQGGIAAVLDPQDSFEKHIEDTYKAGAELGKKKVIQQIIMEGPKLIQYLIDLGTD
                     FTRKDETYDNCLENLSLTMEGGHTFRRIAYAEDSTGHQIMETLTKRCRENPNIDIYEY
                     HIAIDLITQHHIVQDDGFIPGITCWGAYIMDDATKEISIFKARKTMLATGGAARIYAH
                     NTNPDVATGDGMAMARLAGARLANMEFVQFHPTAFWSPTGDTFLISEALRGEGAILRL
                     QDGSTFMEKYHPQGNLAPRDVVSRAIDAELKKRGEKFCYLDATSIPAEILHKHFPHID
                     SRLKSYGIDFTKDPIPVFPAAHFFCGGVLATIDGITDIKNLFAAGEVACTGLHGANRL
                     ASNSLLEALVIGWRAGNHPSNLEEVTFPEILPWKQMEEFNEHEWVVISHNREIIGTIM
                     DGYVGIRRSRRLLKYALSRMENIYNEVNNFYQHNAVRREVVETRNMAVIAIAVIRSAL
                     MRKESRGAHFLIDAPVPNDELYKIDTII"
     gene            75585..76226
                     /gene="tmk"
                     /locus_tag="PK015_00355"
     CDS             75585..76226
                     /gene="tmk"
                     /locus_tag="PK015_00355"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOL32954.1"
                     /translation="MEFMKGIFITFEGIEGSGKSTQTTLLCAALENLHYKVFRTHEPG
                     GPPIAEEIRELLLNTKNKEMLPETELLLYSASRAQHTGEWILPALKDGKIVVCDRYYD
                     STFAYQGAARSLDMEFIRILTDFATYHTNPDLTFLLDLSVEEGRKRIKNRYPDRLESE
                     KDEFHQCVRNQYLALAREYPERFVVLDGSLPKETIHNMVLTKVLDYIGAQCEL"
     gene            76216..77814
                     /locus_tag="PK015_00360"
     CDS             76216..77814
                     /locus_tag="PK015_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOL32955.1"
                     /translation="MNYKKQRRAIITIVVVWILSATMLFTATSLFAQNKSSGSDLYSQ
                     LGLFSEVLQKLKQNYVTELKDEELIKAAINGMLSSTDPHTTYFPKEEFEEFTTSTKGS
                     FGGLGIQIDKIGDYITVISPIEGTPAYRMGITTGDRIIKVDDISLIGASTDEAIKHMR
                     GEVGTTVTITISRPGVEKPLEFTLTREVIKIKSIPYYFKLDNGAGYIRISQFNENTVQ
                     ELRTALNSLENEGIRALIIDLRWNPGGLLDQAVDTVNEFIGPNKLVVETKGRIPTSNR
                     QYFTRYNTKSRNYPIIVLVNEASASASEIFAGSMQDWDKGLVIGKPTFGKGSVQQLIP
                     LMNGGGLKVTTAYYYIKSGRCIHKNINDKILLGKEVTEKDQEAEKEKNLQQVYYTVNK
                     RIVYGGGGITPDIEVDADLMTNFGMELRRQNAFFNFAVDYMVEHQHKLDKNFVVTDEL
                     MNRFLNYVEDKGISYTQADLDSTDTYIRTMLKSELIRNVYGDTEAYKASINLDKQLQT
                     AINLLNKYPSMDALFDYAATQNKR"
     gene            complement(77936..78604)
                     /locus_tag="PK015_00365"
     CDS             complement(77936..78604)
                     /locus_tag="PK015_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL32956.1"
                     /translation="MAKDETNMNNRTKQWQHIAILLAALGTGYHLHSVAWELAGFSAI
                     TLLFFFVPIAVGKQNQLRIPLFVQIILLIMIYFPIVYPPIASIPHFEFILHCGSSLTT
                     AGIGFLLIYSQSGYDIGEPVLTPSFIAWFTLCFAITAGALWEIVEFLIDSLFKTDMQR
                     SRSLEIIYGIKDTRLGVLDTMEDLLIDGAMSLLAFYAVKIGLGILLRMNNKKVNLKEE
                     KGIE"
     gene            complement(79304..81784)
                     /locus_tag="PK015_00370"
     CDS             complement(79304..81784)
                     /locus_tag="PK015_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL32957.1"
                     /translation="MKTKHFFVILFCMFLFIFAACQKDDADHQPINTDIVTSLNNYWQ
                     YQSDLTSTLESHDGTMVGIQNAILNLGNKSKNRYDFSQIDGMVDEYYNQCNVISDHFA
                     VLARAEDSIVPYGESKGLLSNVAKGIYNKAKDTVVSGGRMVRSGWRVLSGKQSIRQVL
                     NDPESGIPLLSGWAETIQKRNNARDAKIREMILNWNPTTSPEDCNYTIPYDDLPGSTP
                     QEKANAYLNLSDEDPIKMQTRSGVMLWSDDERAATAETAKELGETGVKAVGDAYGGNV
                     GEWNNEILDQMMEEGETPQDCGTLTVDINSSETGNPPIETGKTLIISRSDMPEDNPRI
                     TIIMNAPQNLQLQLPESEYQVIALAQDFIRGVYDNLQIAQAGLTQVATNLLKLAENAI
                     IIEDLTVVEGCITVNQPVHAHTTCVSTIGQALNFQWTVSGGTYTGFKAKGTDLTFTPT
                     EEKEYTIAVEISDNMGNHKSRSITVASSGGKLVIDNWEIEDENFIDNKLNPGERATIK
                     LFVTNTGTTSLTGYQGIVTSVGFSSSFNPSNVTIEAGQTLPVSVPITINKELSQNQLT
                     LQYQFTTQNQNNDNVLITDTIELPIDFYVTINEISNVVTNRIVNINGKIANPQLTTAL
                     LILDNDSEHTFDLNLNNGNFSQEIVLTGSNEEVQHTVYVIAVSGALVAENTMSFSSQV
                     PLMALHCTLTWDTYGTDVDLWVTDPNGERCYWAHRQTASGLSLDVDDVNGYGPENITT
                     PSVIPGDYLVQVHYYSDHDSENAIGTNSVVVIRKGEQNPVNYYGYLSDSGDLWDVTVL
                     HFDTVKGWSIKATDKHSKVNASTLPPKK"
     assembly_gap    81932..82031
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            82355..83623
                     /locus_tag="PK015_00375"
     CDS             82355..83623
                     /locus_tag="PK015_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HOL32958.1"
                     /translation="MNNQILDFLDFLNGSPTSIQASHEIVKRLEESGFKALKEEEQWA
                     LQVGGKYYVCRDTSVVAFTLGSKNLLDTGYKIGAAHIDSPSLKIKPESLKTENGITRV
                     SVEVYGGPIIGSWIDRSLSIAGKIAIKQEDNLRFLPVNLKQPVAVIPNAALHLNREIN
                     KGFEYNKQIHLQAILQSKESEKNPLMEALAKAAGVSVENISGSELFLYDFEPAEICGL
                     DQSLIMSARLDNLGMTHSIMSAIREVDNSESTCVAALFDNEEIGSETRQGADSSFLEN
                     VLERIALNIGFSREEYLRILSKSFLISGDMAHSYHPSYPEKYDPSYAPVMNGGPVIKW
                     NANYNYATNLESSQRFKRLCEIAEVPCQTYIIRSDLLCGSTVGHIISSRLGIPVVDVG
                     NPLWAMHSVRETVGVEDHINMIKVLKVYYQ"
     gene            83643..84893
                     /locus_tag="PK015_00380"
     CDS             83643..84893
                     /locus_tag="PK015_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOL32959.1"
                     /translation="MATFKPFKAVRPLPEYASEIASLPYDVMDSDEARIEAQKHPLSF
                     IRVEKPEVDLPPEIDLYDPAVYAKARENLYRYLQEGYMKQDEKPMYYVYREIMNGREQ
                     NGLVGLVSVDEYMSGVIKRHELTREDKEQDRIRHIDACNAHSSPVFLTYKHQDSINEV
                     VERVKKNHAPEYDFVADDGISHSLWLIDNPEDIEVIQKSFAALDCLYVADGHHRTASA
                     AKVGLLRRQQHPDYTGEEEFNFFMAVIVPDNQLKIYDYNRIVRDLNNHTVETFLHLVE
                     DKFEVTKLPAVRVFRPTHKHEIGMYLAHCWYCLRPRYGTWDASNPVASLDVSILQNNL
                     LRPILGISDPRRDKRIDFVGGIRGLEELVRRVNSGREAVAFAMYPTSVEELMRIADAG
                     EIMPPKSTWFEPKLRSGLFIHLLE"
     gene            84890..86197
                     /gene="rimO"
                     /locus_tag="PK015_00385"
     CDS             84890..86197
                     /gene="rimO"
                     /locus_tag="PK015_00385"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOL32960.1"
                     /translation="MKYYYIVSLGCSKNLVDSEIFANILEKAGYQPWDESEPIDLVLV
                     NTCSFIESALKEGEQVLSELVEMKREGKIKKLVVTGCLMNRGLGEFEEAFPEVDSWIG
                     LKDFAAMETLLGLTSQAKYQRTSIDEVYHRYLLISDGCSNHCTYCTIPSIRGEMKSIP
                     IEVLVEEAKEIADEDNKHYSELIVIAQDTANYGVDLYGYKALPELLEELIKLNKYKWI
                     RVLYMHPDHFETSWLELWEKHPELLPYFEIPIQHISDPILKAMGRKKGSTELKEMFNK
                     IKDKLPQSVLRTTLMIGFPGESRQDFELLKNFVNSTPFLYLGIFPYSREEGTPAAQMD
                     HQIFPRTIRTRYNQLVKLQQQSLEEIMGSYIGEILEVLIEDYADDIEEANYIGRTWFQ
                     APDIDGLTFVKGENLELGKIYPIKLIDIIGTDLFGEVIKEGVK"
     gene            86194..87186
                     /locus_tag="PK015_00390"
     CDS             86194..87186
                     /locus_tag="PK015_00390"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOL32961.1"
                     /translation="MKVALTGIKPTGVPHIGNYVGAIRPALELAKTCEARYFIADYHA
                     LNACKDPEEIRKMTLEIAAAWLASGLNPEKVLFYKQSLVPQTFELMVILMAFTPKGLM
                     NRAHAYKAMVQANLESGKDSDDGVNMGLYTYPVLMAADILLFDTDYVPVGSDQFQHIE
                     MAQDIAQSFNYNYGKEYLKIPQAIAREETKTLLGLDGRKMSKSYGNTIPMFASEEELY
                     KLVMRIKTNSQTVEEPKDPETCTIFAIYRNFATPDQTEALRRRYLAGGMSWKEAKEEL
                     FNVLNAHLTPLRERYNELISQPDDIKRILEEGSARARELAAKKIKELRQVIGVD"
     gene            complement(87232..88932)
                     /locus_tag="PK015_00395"
     CDS             complement(87232..88932)
                     /locus_tag="PK015_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HOL32962.1"
                     /translation="MTFIDVLNLVGGLALFLYGISMLSDNLQAVAGKKMRQILKRITG
                     TPFKGLLVGLGVTAIIQSSSATTVMLIGMVNAGIMSLQQAVGVIMGANIGTTVTAQLI
                     AFNIGDIAYFFVILGVFGLFFKKSRKMEYWSLIILGFGLLFVGLNVMSNSVIDLKNSL
                     FARNILAQAADYPLLAILAGMIFTMIVQSSSAAVGIILVLANTNLISFDGALYLVYGS
                     NIGTTITAWLAVLTSSSTSKRVALIHTLFNVFGSLFFAILTYLGYFNRFINLITPGNV
                     YAHQNVARHIANAHTFFNIFNALILLPFSGLLVKIAQKIIPQGPEETISMGEPKFLDY
                     HLIDDSYLAIEQCLKEMREMLRLVRLELRTSYEAFKDKNYKKQESIHRIESAIDNLQK
                     EITRYLVAVNERTNSEEIIQKIPALLHTVNDIEKAGDLTEEANRILNQEIPSHNNNIS
                     DDLKKMIGDLYRKVMEMLEYCIEYLEELKPVYIYKIIELEGRINETHNSLREHIMNCI
                     QSGECDAINGLHTIDFIDTMERIADKLKNLVKAGSHDFTYNIHNDELWEINKGELDDD
                     "
     gene            complement(88995..89645)
                     /locus_tag="PK015_00400"
     CDS             complement(88995..89645)
                     /locus_tag="PK015_00400"
                     /inference="COORDINATES: protein motif:HMM:NF013772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL32963.1"
                     /translation="MINRISRISTTSIELRDKSVIKYFSSADSYWKEFKVYEKGLPMV
                     PKLLCSEEPKWIEIEYVEGKPYLNVQPQTEIIDLLADTLGNFHKAMRTDNLYLCHWDN
                     QPSNILYSRNQIWLIDFSESRTSVVEDDITHLLLFWCAEFSSDKLRNLVDKFISRYQI
                     HIPLNAKIWKDALTRSLIRFNQRRSSFGHSLHHLEEEQFNLNVNYLSNLFNSSSSN"
     gene            complement(89642..90466)
                     /locus_tag="PK015_00405"
     CDS             complement(89642..90466)
                     /locus_tag="PK015_00405"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HOL32964.1"
                     /translation="MAKSKKKTITIVIVAIVIMIAIYFIISVIQPPSLDNPESIAWDG
                     TGSRFLISNTGNGKILYTTDFNQYEVLINEGLSSPRGIKFRTDTLYVADNTVIQIIDV
                     KEAKIVESIPIPGSKMLNDIECDKEGLLYCTDTGANKLFIVNPHTKEINSFISPLMTK
                     PNGIVFDGPRRQMFIVCFKPASPILAFNIDTKTFSIFKETLYDNLDGIAIDDLGRIYY
                     SSWGEKCIFMIPQEQNRTIIWQKDLKAPADIYYHLPTNEILVPLFEDNEIKRFKAD"
     gene            complement(90475..91857)
                     /gene="cysS"
                     /locus_tag="PK015_00410"
     CDS             complement(90475..91857)
                     /gene="cysS"
                     /locus_tag="PK015_00410"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOL32965.1"
                     /translation="MQFYNQLKRQKEEFTPINPGEVKMYACGPTVYNYFHIGNARAFI
                     TFDVLRRYLEFRGYKVIYVQNITDIDDKIIIGAQEEGIPFNEFTAKYIQAFQEDCATL
                     GIKPPSYQPKATEVIPDIIDAIQLQIQKGFAYEIEGDVYFDTTALPEYGMLSGKKMED
                     LIPGARVEENALKKHPTDFALWKRSKPGEPFWESPWGKGRPGWHTECVVMSRKYLGET
                     FDIHGGGIDLLFPHHENELAQALAMSGKPLANYWLYNGFLNIEGEKMSKSLNNFFTTR
                     DILRQYSAEAIRFFFLSKHYHSPIDFNKEIIEESERAVNNFYSALKSVDYLNIEVQPD
                     GSCAYQEQAFIQAMDDDMNTAKAVAVLFELTHQCKNISLTRSEREQAALMLVKLGKVL
                     GFFQNLSQRLKETLPDLSQELIQLLINYREQARKEKNWVLADKIRNDLNSLGIEIKDT
                     PEGCIWTLKS"
     gene            complement(91867..95736)
                     /gene="porU"
                     /locus_tag="PK015_00415"
     CDS             complement(91867..95736)
                     /gene="porU"
                     /locus_tag="PK015_00415"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HOL32966.1"
                     /translation="MKKTTLLILCFLLALQVANASFRVLETKQEQIIIEYNLGEYSFA
                     KQNDFVYLVTSNMNYSMEPGAPMLAYEETKIAIPPSGSIQVTLLSSRHHSVKLEKRIM
                     PVPEVVMEQDVSSYQYTLNEQLYQLSKSNNPLLNLLNPTTYRGIGFCPLEIHPFSYDG
                     NYNLEITDQAIIKIEILGNTSFRSIMESDDLTDLFLSQIINPEQAKNWQYITPAKVEY
                     AEFSRSDYWWKIETDREGIFKIAPSQLTGFPLSDIDPRSFRLFSNSGVLLPFAINSPG
                     NPFQEIPIQVIGEEDGRFDAQDYILFYGTTRDGVEKNQILQSNKDTYYNPYSGNCVYW
                     LTFGGEFNTDPLRIATLPELQTWNTQTSTFVDNVRLETEKLRRETIGFDWYMTLLAGK
                     VTADYDFTIELPDLSTNDNGILSFSLRQESVNYIATHNISVYVNGNVIPSSANSYIFS
                     WQGLNEYVFQKSVPYFVPGVNTIRIRVHRDKTDNIFLDWINVKYTRNIVKYAGKQTIV
                     NQLENEYVVPVRYNVSSSTNFTVYQINSFNEASLVPVKNIAGSYYYIASGNTATKFIL
                     TNEEYYLPAKVQFIEPVDLVSNPGQVDNIIITPDEFINQAQTLADKYNLYFNKRSKVV
                     RQSDIFNQFNSGHPDPEAIRLFLSYVFQNYPTPRITSVTLLGLGTIDWRNFSGQAQSK
                     NKMIVYQRDNSTSDDFFVMLTQTYNPELAIGRYPVTNLNEINIMFSNFSSYVENPVGG
                     WWKNSMVFVADDLYNGSEPYYENYHTQQTETLSNTIHPSILIDKIFGWEYEYDEFQNK
                     PKARDDMMAAINEGRLVWLYVGHGGHDQLGAEDYFNGATDMGRFNNPGKLTFFIAASC
                     EVSKFDYWGYESLGQKTVLLNNLGAIASLGATRMSAAGSNVGLTTFILDYLANKRNPL
                     GYSIMAAKTAYTQSTINDALYVLLGDPLLHIVPPVRDSILTIFDPDNYKTKENQGILY
                     ARQKVRFTGSFSPSTSNGIAEVKVFNNKLVYNLDPQTIISHRGAPLFVGSSTVNTGFY
                     QSGFIVPDDVTTGNSGLIVSYFWDPNLKQSYTNYYYPLQLSDEAVSANNPDAPHIEIY
                     LDSMDFRPGDIVGTDPILYARISDSNGINVTGSAGHNILLILDNSLQPVSVTNYFRYD
                     TDSFTQGILTYPLSGLSEGVHSLQIIAFDNFNLPAVATTHFQVRKVAELYIERFLIYP
                     NPMKSTTNFTFMLPRDCELTIDIYTISGKKIYSMKTMGRQGFNSIPWDGRDNKGDKLA
                     NNTYFVKIRAKDGKEKTEKIEKLVIYH"
     gene            complement(95733..95888)
                     /locus_tag="PK015_00420"
     CDS             complement(95733..95888)
                     /locus_tag="PK015_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL32967.1"
                     /translation="MTFTALVTVVENFKYSLGILVEGMAGIFIFMGVFYALIIILNIV
                     FKQKKEQ"
     gene            complement(95903..96367)
                     /locus_tag="PK015_00425"
     CDS             complement(95903..96367)
                     /locus_tag="PK015_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOL32968.1"
                     /translation="MKIKILQIGKTKEKWIQQGIQMYLTRLEPYLKIEVEEIPSVSIL
                     NADSQDMVKAKEASKCLKRINSDDYVILLDEQGEEQTSLEFANFLANLSENKKIVFII
                     GGAYGTHQSIKEISNYSLKLSAMTFTHEMVRLILLEQLYRAEMINHNRPYHY"
     gene            complement(96364..97266)
                     /locus_tag="PK015_00430"
     CDS             complement(96364..97266)
                     /locus_tag="PK015_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HOL32969.1"
                     /translation="MFKNFNEMVASVKAKRRGTVIIAAAQTESVLQAAIMAQEENLAD
                     CLLVGDKSLILSLLGKLDPNHHYSFEIVDVGSDLVKAAEMSVQLVNEGKGDIILKGKT
                     DSGLLLHCVLDKEKGLRISPVISDVLAYEHPEGVKLLSDGGVNIAPNIEEKIAIVKNA
                     VEVAHCMGSSQPKVALLAAVETVNPKMQSTLDAAIISQMNKRNQITGCIVEGPLAFDN
                     AVSLEAAKIKGIESPVAGNADVFIVPNIEAGNIFGKLLTYYCGYRVAHVIVGTKAPIL
                     IPSRADPGELKMLCMAMALTCMQE"
     gene            complement(97303..98178)
                     /locus_tag="PK015_00435"
     CDS             complement(97303..98178)
                     /locus_tag="PK015_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOL32970.1"
                     /translation="MNRENVFADKALQNYLKEISKFKTLSREEEHELALRAREGDQEA
                     INKLMQANLKFVVKIASQYQNRGLSLSELISEGNIGLLKAIEKFDPDQDIKLISYAIW
                     WIRQRIMQAVSEKSSLIRVPMGKSSSAHRVKATEDRIYSETGETPSIIELSERMNLPE
                     KSIEQLKDQIVETTSYDELLQNGDFQEFTTRDLIKDEGLVDPQEIYSRERLQNKIENA
                     IKKLDKREADIIRTYYGLNENHETRNFAQIAETMGLSRERVRQIQKEALKKILAELQP
                     EEDKLVDEFLEKYSY"
     gene            complement(98190..99431)
                     /locus_tag="PK015_00440"
     CDS             complement(98190..99431)
                     /locus_tag="PK015_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOL32971.1"
                     /translation="MIFKYYKRLDREEKDKFLDIRPPGKMTLFLLFLLLPFILSAEYI
                     THKVQPKDTLYSLSKKYDVPIEQIQKLNGLKGNIIHEGQVLKIKEEEKTKTPEVPAII
                     PAKPSLSTIQDKDSPTTPIIQKAELADKESIVFPEDYYYKVRKGDNLYRIALNNKIKL
                     ADLLKWNNFENESHTIKEGDRLFIKDPSLYQKKTTLDAPKIETNTPEDPDSLIIEKIY
                     VVQKKDTLYKIARENGMTVEELKALNNLKSDKLSVGQKIWLVGKPRTLEEEEEEELKL
                     PSSSGTLKSEQINTNCIVPAEGYVSSEFGLRRGRPHKGIDIANKTGTPIYAAMDGVVL
                     FAGRQNGYGNVIILEHPDFIMTVYAHNEINLVREGDNVVQGQQIATMGATGNATGSHL
                     HFEYRVKGNAINPRKVLPFKK"
     gene            complement(99412..99681)
                     /locus_tag="PK015_00445"
     CDS             complement(99412..99681)
                     /locus_tag="PK015_00445"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOL32972.1"
                     /translation="MHSWKIIARGRVQGVGFRHYAQTCARRCGITGYVRNLYDGSVLI
                     EATGDQESLNRFCELLRNANRFIDVRELEITEQEDTVMYNDFQIL"
     gene            complement(99929..102826)
                     /locus_tag="PK015_00450"
     CDS             complement(99929..102826)
                     /locus_tag="PK015_00450"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOL32973.1"
                     /translation="MKKWVIFLATLLLASSLMALPSILAIPVQQIMNRYTALSTYEAM
                     QQLSQQGVQILHYDSEYAIIIESDKAKDFPGALKIGDISILPDLYLVSKIPEESFSLP
                     SSVGTILLNLRSAYLIQSSLDEIILRETIKHPFTHLGPEPIIFSDIKLLPEKRETFRT
                     DIAQIISLVDADSILYNIQCLQDFQTRFALADNRFQVAEWIKNRFISFGINDVQLQSF
                     IWNNTDQYNVVATIPGSLYPEQYIIIGGHHDSITYGPSENPFVFAPGADDNASGSGGT
                     LEIARVIMQSGYQPRCSIRFVTFAAEEFGLWGSKYYALTALNNNENIRLMINHDMISN
                     NNDPYFYRVILNPYDGSENYTNYALQLIEQYTDLQVYYNYSVNPSNSDSYSFWQKGYN
                     VLYFSEFDFSPYYHTTNDLTQHINSDYCAEVIKASTASAVRFSEIPIAPINLVVRDTG
                     NGSSLLVTWESPYEPVLDHYNLYYSTVFGQWNEPISTTQTSYILDNLTEGQMYYVGVS
                     SVDFNGLESFIIVATGTPNLIPLTPENFKVEPVYRAVSLSWNENLELDLAGYNLYRSQ
                     SEGELGIQIGGLLTQNSFLDRDVIGSENYYYYRLCAIDQDGNRSEFTSAIKTRPVTLN
                     KGILIVDETENMSGNNPFQPSDEQTDIFYADIMQNFETHQLDLNELSETLTLADIGIY
                     SSILWYGNDLASMDYPYFIQDDLKKYIDYGGNLFFSVYHPSLAFALNSSYPNYFNSDT
                     FIYQNIGISETDYSSAARFKYAIPCFEDFPPLEVDPEKTISSFNGHIYQIESIEPSPY
                     ANAIYLYGSDYDSETPQGSLNGSTVGIYNPNQNGKVIVLSFPLYNMNKTEAQKLVEYS
                     FHHCFNEPGISTDLTGDNKILIFANYPNPFGEKTSFRIEWLNADQPVKVEVYNLRGQL
                     VKTIYKGYSPKSLIYEWDGTDNNGKTVSSGIYSIKASQGKKNSIRKVIMIK"
     gene            103015..>104108
                     /locus_tag="PK015_00455"
     CDS             103015..>104108
                     /locus_tag="PK015_00455"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOL32974.1"
                     /translation="MKIRLVSLVIISVLFSVLMAENPQNINLEMKMIGLSDVPSEEEV
                     YLGVYIQDLDYPTAQALGYNELYGILITKVEKGSPAWNIGIRKNDILMEINGYPIFNV
                     KEFESIKKLLHPGDIIWLHIWRDGNIFDITMKVQGRTKEKSDAKKQPKSKESYPVGWG
                     GGSWIPYWVVFPVDDVNELIAHIGEPTGDKTFANNAISEDGVFMSGGGGKINIGIGFF
                     LGGVGASYSFSDSDASTHSKIKYNLSFGGITLDKRYAFNRKLIGSLGIMLGSGGHQVE
                     YSALANTDFNWPEISSSGSFNVKVSREYFMIQPRAEMLIRLLPWLAIQTEVGYMYGIP
                     THSGWKMQATTGDIYSIDNSPDTPFHSLTI"
BASE COUNT        33114 a        18187 c        19776 g        32731 t
ORIGIN      
        1 ctgtcctggg cgggtcaaga attattacct cagggatttt agatgtgtat ttttcgggag
       61 aattaagtat taacggtagt gtatcttcgg ttttcccatt aagaaaagtt gcattggata
      121 tatcatttaa ttgacagttg aattttccat cgtcaatagc agcagatgat tcttcaatgc
      181 aaaaaacctc tcggacttta gctgccagag ataaacctaa gactccaata ccagaaaagg
      241 tatccaagac agtgtcttca ggttgcactt taccttgtat attttttata ataagttgca
      301 acatagcaga attgacttgc cagaaggact tataatgtat cctgaatttt aaattgttta
      361 tttcttcata tatataagga ttgccataga gaattttttc ttcattacca agaataacat
      421 ttcctttttc acgattgata ttttggacga ttccggaaag ttgaggaaat tcagaagtca
      481 atttctttac caacaaattg ctgaaaggta gcttagcact tctggtaacc agaatcataa
      541 gtatctgttt tttatctttg gaacatcgga aaccgatatg tcttaaagtt cctgtatgag
      601 aggtttcatc ataaggctgg acttttgcat tatccatcaa ttccagacaa cgatgtgcta
      661 taaggtcaaa ttcagacgga tgtaagaaac aggttttatg cggaactatt ttgtgtgacc
      721 agcgttcgta aattccaaat tccagtccat tttttccttt gcttacaggc ataaaagctt
      781 tattacgata atgctgaatt actggggagg caatagtatt cggcatatct atccggggat
      841 ttagagattg aatctgttct cttaccaatt ctgttttata ttttaactga gtttcatagg
      901 aaagattcag ccagtcacaa cctccacaaa aaaaaggtgg tccaaaagaa ggacagggtg
      961 gctcaattgt gccaggacct ctggtgatat acttctgaac tttggcgaaa gccacatctt
     1021 tttttcgggt cactaactga acttctacca catctccaat agccgtgtag ggaacgaata
     1081 tagttttccc ctcaccgtat cctattccaa atcctccagc agcaattttt tctatactta
     1141 gcttaacgag aaccttcctt ttccatcatt ctacggagtc tttctattct tttctcaatt
     1201 ggaggatgag tggaaaaaag cgtggcggat cctttatagt tgattttggc aatagtataa
     1261 gcatcttttt ttgtaggtgg cacgaattta ttgctaatct tttccagagc tatcatcatc
     1321 tgaacaggag aggttaatct ggcagatcct gcatcagcaa catactcacg atgacgagag
     1381 taagcacaaa tggcaatatt ggcaaaaatc atcagaatat tctgtatcaa gatagcaaaa
     1441 aagaactgag ttcctcttcc aaagccttgt tctctatttc ttccaccact tagaattata
     1501 gccagaagat gggagataaa aatgacaaaa gtatttatag tccccatcaa aagcgtcgta
     1561 gtgaccatat ctccttcagt gatatgagtc atttcatgac ctgcgactgc acccagttcg
     1621 tcaatattca ggttctccat tattccactg gaaaaagcaa tcaaagaatt attacgagag
     1681 gctcctgtgg caaaagcatt gttatctggt gaaggataaa ttcctacctc aggcattttg
     1741 atgtgtttat aggcagccat ctgttctatc atctcataca acgcaagtgc ttttcctgtg
     1801 gcattttgac gagttatcgg tgttatccga taagacgatt ttgccatagg tttactaagg
     1861 aataacgaga taaatgaacc agcaaaaccg aatatggcac aataaattaa taacgatatt
     1921 acattactgg gattaattct taaaagctga agtattattc ctaataccag caagacaagg
     1981 aagttaatcg ctaaaaataa cccccaacgt tttataatag ctttcattgg attactgtct
     2041 ccttatttta gtaacaacat tttacgagtg ctggaataag caccatttcg cattctataa
     2101 taatatactc cgctacttac aattttcccc tcattatcta aaccatccca gaaaacttta
     2161 ttagttccag ctgtaaaatt accatttaac agattacgta ctaattcgcc tttctgatta
     2221 taaatatcta taatcacttt tccaggttct gcaagtgaga aatggatttc agtgctggga
     2281 ttgaaaggat tgggatagtt ttgaccaagg taggtatgag gtgttagagg aggattatca
     2341 tcaatacctg aaacaatgaa agtgaaagag gaagtagtat cactttctac atttccataa
     2401 agtgctgtgg cgtaatagat atgagttccc agaggaatag aaatatcctg ccaggttatc
     2461 atcattgacg ctggtgctgt atgaactact tcattatcac gataaatctt atagccagtt
     2521 aaaccttctg gaagaacaac attggcttca gtctcaaatc cgaccgtgat ttcaagggca
     2581 cttggagtta atatagtatg agtaaatact aaaggaggca agggctccga tatctggaaa
     2641 tcagctttat atcttgcagt ataatagttt ccatctgtgc gggaattggg gattcccaca
     2701 atatttagta aataggaata gaccggcaag ccaatgtaat ccacatcata aaatgtggta
     2761 cggaagaagg cagtattatc gcctgtggca tctatcaata aataggcaag cccattagca
     2821 aaagttccgg tcggttcaaa aaatctaatg tcagagattc ttaccaatct ggattcatag
     2881 gtctcaaaat catccagaaa ctggagtata tcaatgtcta tgggagtgat tgtatttcca
     2941 gtagaactgg gtgtaccggg atctactaca gggactaatt ccagcatacc tccaaattcg
     3001 gttaatgttc ccgttagatg actaattcca tctccgattt gataaacagt agtgataatt
     3061 ccattgttat catcaatcaa gataccagca gtggaatcct gcacaaattt ttgatgacga
     3121 tagctttgag tgaaagtaac taaaacttcg ccagtcaaac gatatagtcc atttaaaggt
     3181 gattgacgta aagcagcgag attgtttacc atcactggga aagtataggt ggcagaagtg
     3241 acggcagaat attcagcagg acttatatag gctattgcct taaccgttgt agtgctggaa
     3301 atggttaggg gagctgtgta tagtgttgaa ttaagatttg gtgtgcttcc atctaaggta
     3361 taatatatat tagcattggg attggaacag gttatagtta gaaagaaact ttcccagtag
     3421 tatcctgaag ggtgactgaa cacaggagca gcaacaggtc cagcaggatc agaaaaatcg
     3481 cttaagcttc tgggagtgaa ataatctccg tcggtccttg aattaggaat accggttata
     3541 tctttggtag tagttggaat tggagtattt atgtaatcca catcgtaaaa agtggttcta
     3601 atgttatagg aagcagtggg atcgctggtt ggatagactg ttccattggc aaagttacca
     3661 ctgggattag taaaattaac actcaaaact ttgactactc tggattcaaa agtatcaaaa
     3721 tcatctatca attgctgata agtggcaatt attgggacga tgagattgtt tgtagaagtg
     3781 gcaggaccag cattaatcac aggaacaaat tgtagcatcc ctccatactc actaattttt
     3841 ccagttaaac cagttatccc atcacctata ttataagtag tagttatcac gccattatta
     3901 tcatcaataa ggattcccgc atcatcatcc tgtacatatt tctgattacg aaagctttgc
     3961 tggaaggtta aaattacttc tccggtaact tgatatactg ttacaccatc tgctggttgt
     4021 gaacggagtg tacttaaatt gggaacggtc aaaggaaagg tgtatatagc agtggtgaca
     4081 gagctattac tcattcctgg agctatagca atggctttta atgtggttgt gactgagata
     4141 gtgatgggtg ctgtatagag agttgatgaa ggagttggaa cacttccatc cagagtataa
     4201 taaatgctgg cattgggtgt ggcacaggtt atggtgacag tgactggaga ggtataaatt
     4261 cctccagcag gattgaagac tggaggagca gttatatcac caccacctgg tgtgaaggta
     4321 tgcattccca gatccgttat ataatcttga ggatgaacta tccattcact atcatctgca
     4381 ttagttccag cagagatagt ccaatctgta ttaccttgaa taacagtcgg cttacggatt
     4441 aatgtgtgat tcagtgtggc agcgggaaca ccagcaacat tccatccaga attcgttccg
     4501 ggatcattct gatatatacc aatgatatca atcaaagtag tccctaaaaa aagtcctaca
     4561 caatcattac cattataatt taccacattt gaagtcacat cagcaacact gagaatggca
     4621 gcactggctt gaggatgagc tattacaaat acatcattat gttgcagaat ggctgtggga
     4681 gttaaatagt ttgaagtgct ccaggtttga ccatttgatg ccagttttat ggtgtactga
     4741 gaaagatcaa ctggagcgcc agtaccatta aaaatttcca gagctttatt ataggaagaa
     4801 ccttccacat attcgctaaa gaaaatggtc tgagcataag gttgagcgaa aagaataacc
     4861 gtgctcagca atattaatgc aataactatt agatttcgtt tcattttgaa actccttctt
     4921 tttcagagat aggactaatt ctatcccgtt ttatttatta aataatctac ttttcttaaa
     4981 aaatcatttt tcctgattta cagaataagg caagaaaaat tttttattct atatagtgaa
     5041 acataatgaa tgtgtttatg gtagttttat tcttttgagt aagttcttat tctttatact
     5101 aaaaatatgt ttaataactt tttaagatgg attagtaggc atagaggtaa taagaatagg
     5161 agtaaaatgc acaggggtgt ttagttagga gtaataagat aggtagtgat agaagtcctt
     5221 atctattaag ctccgccgga gctttttaaa aataatagta taaagttcaa aaaatacaat
     5281 tacagttact attatttggc ttttaataag attttgatat atagaagaaa agacttatga
     5341 ctttgtgaaa gttggacatt tggcatctct atttttttac ttttacttaa tatctttatc
     5401 tttaaatatt atttgttctt ttttcttccc cctctttttt atttatgatt aatttatgtc
     5461 cagcttatgt ccagcttatg tccagcttat gtccgaaagt ggacataaac tccacataat
     5521 cagtacatta tttttatatt tatcgaaaag ttcaagcttt atcagctata tttttaagaa
     5581 aaaaacttaa agatttaaag ctacattact tttaacttat atttttccct aacagtagtg
     5641 cctatatcat tttttcaact ttcatgaaga ttttgtaaaa ataggtgggt ataaaacttc
     5701 gtctacaaag ctccgcagga gctttttaat ataaaataca aataatttaa agatactatg
     5761 aataaattcc ataattcttg aaagcttaag aagttattac cctttttaag aaaaagcctt
     5821 atggctttgc gatacggaga cgtctcgcac ccctttatgc ggtggtagaa gttcttgtct
     5881 atgaagttcc acaatagcct gctaactata taattaataa cagattaagg ttaggtttaa
     5941 gaaatcatta aataagcttt gaacttgaca gattgaatag ataagaaata aatgataaga
     6001 aaatatattt aaagtttaaa acattcattt gttttgaacc tatggagact cttaaatgga
     6061 atatcccttt tccaagatag aacctaaatg gcaaaaaatc tggcgtgaga aaggaatctt
     6121 tcatcctgaa gatttccctt cccaaccaaa atattatgtc ctttctatgt ttccttatcc
     6181 atcaggaatt ttacattgcg gtcatgtttc caattatgcc ataagtgatg ctatcgcaag
     6241 gttaaaaatg atggatggtt atgcggtgct acaacctatg ggatttgatt cttttggcat
     6301 gccagcggaa aattatgcca tagagcataa ttctcatcca cgcataacca cggaagaaaa
     6361 catacaagcg atgcatcgtc aatttgattc tcttggcttt ggtcttgatt gggaaagaga
     6421 aattagcacc tgccgtccgg attattatgt gtggggacag tatcttttta agaaattgta
     6481 tgaaaaggga ttggtctatc ggaaaaaatc ctggcaaaac tggtgtgcaa attgtcagac
     6541 catacttgca aatgaacaag ttgtgaatgg aacctgctgg cgatgtcata atccggttac
     6601 tcaaaaagag caggagcaat ggtttttccg tatcactgct tatgcagaag agcttttaga
     6661 ttttagtaag gtgattgatt ggcccgaaag agttataact atgcagaaga actggatagg
     6721 taaaagtgaa ggtactctaa tagattttcc tctggaagat ggaactgact ccattcaggt
     6781 tttcaccact cgtcctgata cggtttatgg agtcactttt atggcacttc ctccggaaca
     6841 tccgttggtg caaaaatggc ttaaagaaga gccagaaaat cgggctttga aggaattttg
     6901 tgaacgagtt ataaatgaag ataaaatttc ccgggaagct gttgatacaa ttaaagaagg
     6961 aatgttttca ggtcgctact gtctcaatcc tttgaatgga gagaaaattc aaatctgggt
     7021 gactaactat gtggttatgg attatggaac aggggcagtg atggctgttc ctgctcacga
     7081 tgaaagagat tttgaatttg cccgtaaata tggtattccc ataaaattgg tgatacagaa
     7141 tcccgaacga acacttaatt tagaaacaat ggaaaatgcc tatatagagc caggcattat
     7201 ggttaattcc gctccttttg atggaatgta tagtgaagaa gctaaaatag ctatttctaa
     7261 gtttctggaa gaaaagggat atggaaagat tacctctgct tatagattaa gagattggtg
     7321 tatatcccgt caacggtact gggggaatcc tattcccatt atttattgtc ccaattgtgg
     7381 tattgttctt gttcccgatg aagatttgcc cgtacttcta cccgataatg ttcaagttgg
     7441 caagaccaca cgcaatcctt tactttcagt tccagaatgg tataatgtaa aatgtcccca
     7501 atgtggagcc gatgccagaa gggaaaccga tactatggac acctttgtag atagttcttg
     7561 gtattatgcg cgttatacgg atcctcataa tgataaaaga ccttttgatc cggataaagc
     7621 caatttctgg cttcccgtag accagtatat tggcggaata gaacatgcct gtatgcatct
     7681 tctttatgca cgtttcatct gtaaatttat gcgtgacctg ggctggctaa aatatgatga
     7741 accttttctt cgtttgctca cacaagggat ggtgctaaaa gatggtgcta agatgagcaa
     7801 atccaaaggc aatgtggtag atcccaatta tattattgac cgttatggag cagatactct
     7861 cagagttttc ttgttgtttg cctctcctcc agataaagat gttgaatgga gtgatgaagg
     7921 tattatgggt gctttccgct ttttgaatag gatttggaga ttgatagatg agaatctgga
     7981 cattatccaa aagggggtaa agattgaacc tgactacact caaatatcca caaagatgcg
     8041 gaacctgatt tatagcctgc ataaaactat caagaaatgg agagaagatt gcctggatag
     8101 aatgcaattc aatactgcta ttgcagctgt tatggaacat ctcaataact gtctggctat
     8161 taaagaggtg aatgaacttt cagataatga acaggcagtt tttgcacaag cctgtattgt
     8221 ttttccccct ttgatttatc cttttgctcc tcatatagct gaggaactct ggcaaaaatt
     8281 aggaaatgaa aagcttcttc acgaatctgg cttaccagaa tatagtgaac gctttcttga
     8341 actggataaa attacttatg taattcaagt gaatggaaaa cttagaggaa agatagaagt
     8401 tgacccagat attagtgaag aagagcttaa aaaacttgct ctggaagttg aaaatgtaaa
     8461 acgccatata tctaatcagg aaattaaaaa gattattgtg attcctggaa aaatggtcag
     8521 catagcggtg ggaaaataag ctaaaattga ttatattatc gtaaatagaa aaaattcagg
     8581 aggaaaaaat gtgctgtcat aatgagcatc attgctatga tgactattgt tgctgttgcg
     8641 gacaccatca tagccacttt aatcatcccc atcagcattg ctgttatggt catcattatt
     8701 ataaccatca gagagatgaa gattattgta aatgtgaaga aggagatttt gaacaccata
     8761 aagaacacca tcatcatcac tatgggcagc attttgctcc ctactatgaa tgctgttctc
     8821 aaatctccaa agaaaaactt ttgaaagcta aaaaacatct ggaaagaatt attcaatgga
     8881 ttgaccagca actggcagaa gaggaagaat cgggcaacta atggaagcaa atcaggaaaa
     8941 agagattcaa gcgaatctgg caaaaatcca tcataggata gttgttttaa gtggtaaagg
     9001 tggagtaggt aaaagcttta ttgccacaaa tcttgcttat ggattggctt tacaggggaa
     9061 aaccgtcggt ttgcttgatg tagatattca cggtccttcc attgctaagt taactaatat
     9121 tgaagggaaa cctattccca ttgaccagga aagtggcaaa ccaactccca ttcccgttct
     9181 ttccaacctc tatgttctaa ctttagcttc tattcttgat tcggaaagta cagcagttat
     9241 ctggagaggt ccaatgaaac tggcactaat ccgacaattc ttcagtgatt ttgaatggaa
     9301 agagcttgat tatttgattg tagattgtcc tcccggcacc ggtgatgaac ctttaaccgt
     9361 gattcagacc ttaaataaag tggatggagt agtgattgtt actactcctc aggatttagc
     9421 cattttggat gtgaaaaaga cagttgattt tgctgagaaa ttgaatgtcc ccatccttgg
     9481 tttggtagag aatatgaaat atttccgctg tcctcattgt ggcaaaacta ctcaaatatt
     9541 tactggtaat caattggtag acttagtatt tgaatattct ctggatattc ttgctgaact
     9601 ggaaatagac cccgatattt caaaatctgc cgatgaaggc aaaccttata tctattttta
     9661 taataaaaaa gattctgcta atgctttaat gaatgccgtc ctgaaaatta tggaaaaagt
     9721 ggaaggtata aataatccag ataaaagaga taaagcgttt gacaattatg tataagtgaa
     9781 aaatttggtt tcaataatat cgttttaata ttaaggagta agatagatga aaaagggaat
     9841 tcatcccaaa tatgatattg ctactgtaca ttgtgtttgt ggcaatactt ttcagactcg
     9901 ttcaactaag ggtgatttac aggtagaaat atgcaacaaa tgccatcctt tctacactgg
     9961 aaaacaaaag atagtggata gagcaggacg agttgaaaaa tttattaaaa aatacaatct
    10021 gaaaacagac gaaaaatagt taacaaacta ctataacgcc atttcgctgc aaataagagc
    10081 tggagaaatg gcgtttttgt atccagatta gatattatta aggatttata tggagcaaaa
    10141 gaaggaaatc agtgtcggtg gacaagcagt tattgaaggt gtgatgatga gaggacccga
    10201 tgtaatagct actgctattc gtagaaaaga tgggactatt gaattgttta gagaacattt
    10261 tgaaacaaaa gctaaaaaag gaacttttct tggtctaccc attattcgtg gttttgtctc
    10321 tctgatagaa atgctcatta taggaatgaa gaccataacg ctttcggcaa atagagcaga
    10381 gctggaccta aaagaaaatg cccccaatcc agcaaaagcc tccacagaaa aatcaaaaac
    10441 agttcagcaa ttggaaaatc tttttagcta tgtgattgcc tttgctcttg cttttctctt
    10501 atttggctgg cttccttact ggcttgcaga tttagtaggt ttttccagac agaatttctt
    10561 cttcaatctc tttgctggtg ttatgagagc agttttcttt gtggtatata tatggttaat
    10621 aagtaaaatg aaagatatcc atcgcatctt tcaataccat ggtgcagaac ataaaaatgt
    10681 caatgcctat gaaaaaggca ttcctctgga aattccgcag attcaggcaa attcaactat
    10741 tcatcctcgt tgtgggacca gctttatttt ctttgtcctg cttctttcca ttataatttt
    10801 ttcccttgcg gacactcttg tatctgcttt tattattcat ggcaagattc ctttagccac
    10861 cagattagcc tatcacatag ttattttatt gcctttaata ggtggggtaa gttacgaaat
    10921 cttgaagttc agcggaaaaa atataaatca tccactggta aagattttga ccgttccagg
    10981 tatggcttta cagaagatta cgactcagcc tccagatgat tctatgattg aagtagggtt
    11041 ggtggcaatg aaagcggcac tgggaatgga ccttgatgga cacaatgttc aaataattca
    11101 agatgttacc tgaagaaaaa cttaatagct ataaaaagga actggaagaa cttcaaaaaa
    11161 ctctctcaga tccttctata ataaacgata ctaatcgcta taaagccttg cgtagacgat
    11221 ataaagagct ggaagagatt tgctctgaat gggacaacta tcaaaaactg gaaaaacaac
    11281 ttgcagatac gaatagtctc ttgcaaacag aatctgaccc agaattggaa gctctggcta
    11341 aagaggaatt ggaagagctg aaaaataaat ttcaaacaag tgaagaaaaa atcagagaat
    11401 tgctgattcc tcaagatccg aatgaccata aaaatgctat tgtagaaatc agagctggaa
    11461 ctggagggga agaagctgct ctttttgccg ctgacctttt ccgaatgtat agctactacg
    11521 cagaaaagaa aggctggcaa atccaggtat tgagtgctaa tgaaacagaa ttgggtggct
    11581 acaaagagat tgtttttctg cttactgggg atgatgtata tggtcatatg cgatttgaaa
    11641 gtggtgttca cagagttcaa cgtatacccg taactgagtc caatggcaga attcatacct
    11701 cagctgttac cgtagcagtt ttaccagaag ctgaagatat tgatatagat atagatgaaa
    11761 aagatttgcg gatagatgta tatagaagca ctggacatgg tggtcagagt gtcaacacca
    11821 ctgattctgc agtaaggata acccatattc ccactggaat agtagtcacc tgtcaggatg
    11881 aaaaatccca gattaagaat aaggctaaag cattgaaagt gcttaggtca agacttctgg
    11941 atgcagaaat cagtcggcaa gaacaggaaa ttgcccgttc tcgtaaagct caagtaggga
    12001 caggagaccg ttctgctaaa atccgtacct acaattttcc tcaatcccgt gttaccgacc
    12061 atagaattaa cttgacaagc tacgacctgg aatcttttat gaatggcgag attgatgatt
    12121 ttattcaagc cttaagatta gcctggcgaa atgaaaaagt gaaagaataa atgtctattt
    12181 tacaaatcat actctggtgc ctgatttttc ttgtcttgat gctattttct tttctatttg
    12241 ctggttttga aaatggtgtt atctctataa atcagctgga actggaaagt aaagcgaaga
    12301 aagataaatc agcagctaaa cttttggcta tggttaggca acctgataag gttttaggta
    12361 ccactctctt aggcaataat attgttaaca ttctttttgc atcacttgct acttatgtag
    12421 tagatagata tatcgtaagt tttaatgcaa gatacacctc cttaattgtg ggagctattg
    12481 ttcttatttt ttgtgaagta gtccctaaaa ctttgtttag agaatattct gatgtattag
    12541 ttccagcggt agctcctttt atgcgaggag tctatgctat tttaaaaccg ctggttactt
    12601 gtgtttcctg gatgaacact cgtctccgta atatgcttaa aattacggaa ggaaatagtt
    12661 ttaattattt aaccaaggac gatttaacct atttactttc ggtgacagaa gctgatgcct
    12721 ctgaagaacc tcagatagaa atgattgaag atgccctgga ttttactgaa caggtagcta
    12781 aagatataat ggttccgcgc accgaattag tagctattcc agccacagcg actgtttctg
    12841 aagcaataga aattgctcgg gaagaaggtt ttacccgcta tcccgtttat ggtaaagata
    12901 ttgacgatat tgtaggaatc cttataatat atgattttct caaaaaggaa tgctctcctg
    12961 atacacctgt gagtaaattg acccataaac ctttctttac tcctgaaaac attaatctaa
    13021 gtgttctttt aaggcaaatg caaaaatatc accgtagtat tgctatagta gtggatgctt
    13081 atggtggcac ttctggaatt gtgacgatgg aagatatcct ggaagagatt gttggtgaaa
    13141 ttgctgatga atatgatgaa gaagaagaaa ttgaagaagt tgagcaatta actccggaca
    13201 cctggttagt tcctggagat atggaaattg accgtttagc tgatgaatat aatatccagc
    13261 taccggaagg agactatcaa acagtagcag gattaattct tgaccgtctt gccaaaatcc
    13321 ctcatcaagg acaaataatt aacattgagg gttatagaat tcaggtgtta caggtaactg
    13381 ataggaaaat actgaaagta aaaatacata gaattaaacc ccgaggtaat gtatgaaact
    13441 gatggaatgt gttccaaatt tcagtgaagg aagagaccag agtgttattg atgctatagc
    13501 taatgctatc aaatcagtta aaaatgtggt tcttctggat gttgatccag gagcagacac
    13561 taatcgcact gtttttacga tggcaggtga accagaagca gttttagaag ctgcatttca
    13621 agctatcaaa aaagcaagtg aacttattga tatgagtaaa catcatggtg aacatcctcg
    13681 tatgggagca actgatgtct gtcctttcat acctatttct gatatgacaa tggaagaatg
    13741 cgtggaatat gctcgtcgtc tggggaaaag agtgggtgaa gaattaggga ttcctgtata
    13801 tctttatgaa tatgctgcca ctaaggaaga atggcgtaat ctttccaata tccgggctgg
    13861 agaatatgaa gcgttaccag aaaaagctaa ggatcctgcc tggaaacctg attttggacc
    13921 tcatatcttt aatgctaagt ccggcgctac tgctattgga gcaagagaat tcctgattgc
    13981 ctataatatc aatcttaata ccagagacaa aagaaaagcc agtgaactgg cagctatcat
    14041 cagagaaagc ggaagacttg ccaaagatga gaagggccgt attataaagg acgaacaggg
    14101 taataaagtc attatcccag ggctgtttca acattgcaaa gcggttggat ggtatattga
    14161 aagctataaa agagcgcaga ttagtattaa tctgactaat tataaaatca cacctcctca
    14221 tatagtgctg gaaaaagtgc gtgaactcgc atcagaaaga ggtattgtgg ttacgggaag
    14281 tgaacttgtg ggtttaattc ctaaggcagc tatggttaat gcaggcaagt tctatctgga
    14341 aagaatgggg gagagcaccg gtcttccaga aagaatgatt atggagacgg caatccaatc
    14401 tatgggttta gcagaactta ctccttttga cctggataaa aaagtgatag aatatgctat
    14461 tgccaagaag gatagactca gtgcaatgag tctggaatct tttgctgatt tactttctac
    14521 ggatactcct gctcctggag gtggtagtgt tgctgctttt tgtgccgctc tttctgctgc
    14581 cttaactgct atggtatcta atcttactat aaataaaaaa ggttatgaat ccgtgcaaaa
    14641 ccaagtgcgt gaaattgcgc ctcaagcaca ggatatcaaa cttcaggcta tacaattaat
    14701 agatgaagat accgatgctt tcaatgctat gatggatgca agtcggttgc ctaaaaagac
    14761 agaggaagag attgcgctaa gagaacaaaa gatagaagaa gcaacgaaac aggctattct
    14821 ggtgcctttt cacaccctgg aaatagctct tcaagcttta aaattggctg atgaagttgc
    14881 caaagtggga aatatgaatg ccctttctga tgcaggaact gctgctctta ctgccttaac
    14941 tgccgccaaa gcagctaatt ataatatctt gattaacctt ccgcaaatta aagatgaagc
    15001 ttttaaagaa gacattaata atcgggctat gaatctgctt caggaaagtg aaactcttgc
    15061 ttcgcaaata gagtccttcg tctcagatag actgaaaaat gcctgataga ctttctttaa
    15121 gccccatttt tctatcttaa ttaatatgaa agcgagtttt attcatcttt tttctcgctt
    15181 aaacttgctg attctacagt tcattatctt tgtaatttta aatatcctgg atggtcattc
    15241 cacctggaaa gtactgtatc ctgacaaaca tcaccgggaa atgaatccac tggctcgctg
    15301 ggttttccgc aaattaggag taactaaagg tattataacc tataaaactg tcttaattct
    15361 tataatagga attttatttg ggcattatgc gaaaaaggat accttcagtt taaatgtcat
    15421 tctcagtgtg gcaaatatcg tttttttaat tactgtggtg cataattata aagtttaccg
    15481 aaaaattcat cattctcaaa gaaaaagtga taaaacttca gaattagatg gagaagaaat
    15541 aagttaagat tcccaaagtc ttttttattg acaaaatagt agattatagt ttgtaagtaa
    15601 aagatataga aatattgatt taacttgcta actgctaatt attggagaag ttatgaagat
    15661 tctgataaca ggcggtgcgg gttttattgg ttcgcactta gctgaaagac tggtagaaga
    15721 aggaaatcag gtggtggtgg tagacaattt atctactggt cgtctggaaa atatagaatc
    15781 cattaaagcc aatggtaact ttcattacac tattggcagc atcctgaatc gggaattatt
    15841 ggaaaaattg atggagggtg tagaccaagt ttatcattta gctgctgccg taggtgtgaa
    15901 atatattata gagaatccat tactttcatt gcgaacaaat atcctgggaa cggagaatgt
    15961 tctggaactg gctaataaat ataaagcaaa agttcttata acttcaactt cagagattta
    16021 tgggaaaagt gataaggttc cttttcatga ggatgatgac cgcctgcttg gacctactcg
    16081 tataagtcgc tggggatata gttgtagtaa ggctattgat gaatttatgg ctctggctta
    16141 ttttcgggag aaaagactgc ccgtggtgat tgttagatgt tttaataccg tgggacctcg
    16201 tcaaactggt caatatggga tggtgcttcc caaattcatc aaagctgcct tgctggatca
    16261 acctattgtg gtgtatggta caggaaagca gactcgttgt tttgccgatg tatccgatgt
    16321 ggttggtgct tttataaaat tgatgaatac tcctaaatgt gtaggagaga tattcaatgt
    16381 agggactact gaatccattt caatagaaga cctggctaaa aaagtgaagg agatgtgcga
    16441 tagtaaatcc aggattgagt atatgagtta tgaagaagca tttgaagaag gatttgagga
    16501 tatgatgaat cgtatgccct gtctggacaa agttaaagaa tatatcggtt atgaaccaaa
    16561 atacagtctg gatgatatta tccgtagaat gattgagtat tatgaaaagt aaagttattt
    16621 gtattattct acttagtctg agtgttttta ttgccgctca ggtacccaca acttacgctc
    16681 caacaaactt aatttctatt aatgtgactg gttttgtaga ttctccggga acttatcaac
    16741 tttcctctat cagtcgtctt tcagatgcct taaaattagc tggaactccc tctcctataa
    16801 tccttgaaaa aggacctacc tatataccga ggctttatgc ctctgaggaa aaatcggttt
    16861 cccctaaaga ttccttattt ttaaattttc aagctctaag gagtgttaag ctaacaagag
    16921 gcaaagaaac aaagacttat gattatatga aatttgtccg gatgggtgat ttagagcaaa
    16981 atcctattct gcgagatgga gatgtgataa atgtaggtgc tgttcatacc acggttacta
    17041 tagtaggaga ggtgtattat cctggtgaat atgaatatat tgcaggagac agactttcag
    17101 acctattaaa tcttgcccaa ggctttacct tgagtgcaga caggaagata gtgaatatct
    17161 atcgttataa ggagaattta aaagattttg atttgattac tgtggatttg cgaacgcaca
    17221 aacctgaaga tattataatc tatccttatg accgcataac tattaatcgt gatacggaat
    17281 atagaagagg ctggaatatt attgtagaag gaaatgtcaa atcaccaggt aagtattata
    17341 ttggtgataa caccacactc tatgatatat tgctcctatg tgggggacct aattccagag
    17401 gagacctgag aaacgccatt tatgcaaatc ttcccaatac cgagaaagta gaccctgaat
    17461 ttgaacggct caagaaaatg gatataaccc aaatgtctat tatagaatac cactatttac
    17521 agaatcgtct gcgtcaattt ccgggcagat atagcataga tgtttctcgc acctgggaat
    17581 ctaaaggtag tgaatgcaat cctgtgctgc aggatggaga ttatctttat gttcctctta
    17641 aaatggatat ggtagctgta actggtcagg tggttaatcc tggattaatt ccctgggtgg
    17701 aagggaaaac ctgggattat tatgttaaat cagctggtgg ctacacaaat aataagagtt
    17761 ttaatggtgt tcggattata gatgcacaaa gtggaaactg ggttagacct tcttctaaga
    17821 cagttttata tccaggagat acagtttttg tggcagctaa aacggaatac gatgcttgga
    17881 cccgatttaa agatataatg ttgattacca cacaaataat gacaataata ttgggcattc
    17941 ataccctcac taattaaagg agaataaaat tgaaagtacc attgttagat ctgcaggcac
    18001 aatacgaacc aattatgtct aaagtaaaag ccgcgattga cgaagttatt gctgaaaatg
    18061 actttattct tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntctaaagt
    18181 aaaagccgcg attgacgaag ttattgctga aaatgacttt attcttggtc atcaggtgaa
    18241 agaatttgaa gataagatgg cgaactattt aggtattaaa tatgctatag gatgcgcctc
    18301 aggaacagat gcactggtcc ttgccgttca agctacagga attggtgctg atgatgaagt
    18361 aataaccact ccttttacct ttttcgctac tgcctcttcc atttggcgaa atggagcaaa
    18421 acctcggttc gtagatatta atcccgacac ctttaatctt gatgtggaaa agcttgaagc
    18481 agctatcacg gataaaactc gtgccattat gccagttcat ctttttggtc aagcttgtga
    18541 tatgactcgg attatggaga tagcagaaaa atataatttg aaagtgatag aagataatgc
    18601 tcaaggtatt ggttgcactt gggatggcaa aatgagttgc tcctttggtg atgtgggaac
    18661 tttatctttc tttcccagta aaaatcttgg tgctatgggt gatggtggga tgtgcttaac
    18721 taatgatgaa gaattggcac tcaaactgcg tcaattaaga gttcatggag aaaatcctaa
    18781 atattatcat aaatgggttg gtttaaacag caggctggat acgattcaag ctgctgtctt
    18841 aggtgttaaa ctggattatc tcagtcaatg gagtgaaggt cgtcgggcta atgcagcatt
    18901 ttatgatgaa cagctggcag atattccaca ggttaagact ccttatatcc atccttctgc
    18961 cgtttctatc tataatcaat acactatcta ttgtgaaaac agggatgctt tacttcatca
    19021 tctaaaggaa aaagaaattg gatgtgctat atattatcct ttacctcttc atcttcagga
    19081 atgttttgcc gaattaggtt acaaaaaagg tgattttcct atggcagaaa aagcatcgca
    19141 aagtgtgtta tctcttccca tctatccaga actgacggat gcgcaaaagt cctatgtggt
    19201 tgaggctata aaagaatttt atcaaggttg agagttatga atattattgt ttgcgtaaaa
    19261 caggtgccca atactacaga gattaaaatt gaccccgtaa cgaacactct cattagagaa
    19321 ggagtggaaa gcattcttaa tccctttgat gcctatgcag tggaagaagc ggttagaatg
    19381 aaagaaagat atggtggtat agttacagct atttgtatgg gacctaatca agcggaggaa
    19441 actttaagag aaacagtggc agttggagta gataatatta ttttactttc agaccgtcgt
    19501 tttgctggag cagatacctt agctacaagt ttgactctgg cgactgctat taaaaaaatc
    19561 ggtgactatg accttatttt tactggacag caagctatag atggagacac gggtcaggta
    19621 ggaccaggca ttgcatctca tctggatatc tgtcaatgtt gttttgtgcg gaagatagat
    19681 gaggttaatg aaggaaccat cacacttcaa aggttaatgg aagatggctc cgacaaatta
    19741 aaattgaaat tgcccgctgt catatctgtt gttaaagaaa taaacactcc tcgtctgcca
    19801 tctcttagag gaaaacgtaa tgccagaagc gtgccattga ccgtctggaa cgcagatgac
    19861 ctgggactgg atgagaagga aattggattg aatggctcac ccactcaagt tattaaaatc
    19921 tttactccca aacatgaaaa gaaaaccgaa aaatatgtgg tttcaccaga agaggcagca
    19981 gatttaatag ttaagaagct ggatgaaata actcgtgggg caaagtaatg ctaccttatc
    20041 ctaatattga tccggtgatg gcacaattta ctattggtgg aatcaatttc tccattcgct
    20101 ggtatggatt cttctatgtt ttgagtttta tactgggata catttttctc tggaaattac
    20161 taaaatatag aaaagttaaa atcacccgag accaatatga tggaattatc ttttccatta
    20221 tgctcggagt tattttaggt ggtagattgg gctatgtgct attttataat ctaccctatt
    20281 acctgaggca tccggtggaa attctttatg tctgggaagg agggatgagt tttcatggag
    20341 gtgctctggg agttattata gcctgtctga tttatatgcg caggcaaaaa attcctttct
    20401 attcagttgc tgattgtgct atgcctatgg tggcagttgg gttaggattg ggacgattag
    20461 gtaattttat taatggagaa ctatggggga aggtaacaaa tttaccctgg ggtatggttt
    20521 tccctgatgc tggaccttta ccccgtcatc caactcagtt atatgaactc ttctttgaag
    20581 gtattgtcct cggagtgctc tcttatattc ttcttaaaaa aactaaaaag gagggattgg
    20641 tcttttggtc ttttatcggt ctatatggca tcttccgttt tcttatagaa ttcattaggg
    20701 tgccggatga cctggaaatc tatgataaaa ttggatattt tctcggtttt atgaccatcg
    20761 gtcagattct tagtgtgata atgattatag cagctgctat tggaatatgg acactttacc
    20821 gtaaaaaacc ggaggtggct ttatgaagca tcctgtattt ttatttagtt taactatagt
    20881 tttacttaca gtagtctcct gtgccagtat gcaacctgta gacaagactc agttattgag
    20941 aactgaatgg gagagatggc aaaattttga agcacagggt gtggtgcaat taactaattc
    21001 aggtctcact atgaggagaa tgtttgtcct ttctaaatca cctgaggcta tgcgttttga
    21061 tatactatct ggtggtgtgc tggcagcaaa tccaaaacct ttaatttctg tctattatgg
    21121 tgattatctc gctattcaat gtcctcaatt tcctaaaata gaaatcctgg ctcaaatgcc
    21181 aatctttcct gaaaagttgc cgatttatga tattgatagg gattcacttt ttacggtttt
    21241 tgccgattct ataatcaatc accaggaatt aaacctggaa ggcttacagc taaaattcaa
    21301 ctctgctatg caactggaaa gaattattga acctaaatcc ggaatggaaa taaatatcaa
    21361 tcgtaattct caaggtgatc ccgataaggt aaaattgact atcagtgaaa atacctatct
    21421 agtattattg attgattcta ttagttatgg aaacgcacac atagaacctc ttcctcccaa
    21481 tccaaaagtg gaattaccag aagattggaa acaaatgttt aaaagtttac tggagtaatt
    21541 gatgattaaa cgttatagct tacctgaaat ggagcatatt tggactcaag agaaccgata
    21601 tgagtgctgg ttagaagtag aattagcagc ggctcgtgcc atgcatgaac tggatattat
    21661 tccagatgct gattgggaaa ttattgccga aaaagcggat tttaatagtg agcgtataga
    21721 tgagattgaa gcagtcacgc atcacgatgt tatcgctttt ttgaccaatg tggcagaata
    21781 tgtaggtgaa cctgctcgct ggattcatta cggattgacc tcttctgatg tgctggatac
    21841 tgctacagct ttgcaattaa agcgttcagg tgagttgatt ttaaccgaat tacttcgtct
    21901 ggcggaaact ttaaaattaa aagcaagaga acatcgcaga actttgtgta tgggtcgttc
    21961 tcatgggatt catgctgaac ccacttgttt tggattgaaa ttcgctctct ggtatgatga
    22021 agcacaaaga aatgtgcaac gcttacaaaa tgctattgat actgtagctg taggacaatt
    22081 cagtggggct gtgggaaatt ttgctcatat agacccccgg gtggaagaat tagcctgtaa
    22141 atatctggat ttaaaaccag taaatatttc tactcaggta atacaaagag accggtatgc
    22201 ctatttctta gctgaacttg cacttatcgg tgcattgata gaaaaaattg ctctggaaat
    22261 aagacatttg caacgaactg aggttcaaga agtggaagaa aatttctctc caggccaaaa
    22321 aggttcttcc gctatgccac ataaacgcaa tcctatcgtg agtgaacaac tttgtggatt
    22381 gtcacgagtg cttcgttcta atctaattgc cgctctggaa aatattcccc tctggcatga
    22441 aagagatatt tctcattctt ccgtagaacg cattatcttg cccgattcct gcatcttagt
    22501 gcattatatg ctttctaaat gttgcagttt aatcagcaac ttggtagtat atacagagaa
    22561 tatgaaagcc aatatggaac ttacgcatgg tctggtattt tctcaggcgg tgcttttgca
    22621 tctggttcag aaaggacttt ctcgtgaagt agcttatact atagttcaat ccgatgcgat
    22681 ggaatgtgcc aataccggta aagacttcaa ggagtatgtg ttagcagata gcaggataac
    22741 ttctctcttg cctgaagcag aaattaagga tatctttaat tatgagagat atctcagaaa
    22801 tgttgatttt atttataaac gctgcgggat tatctaaact atgaagtaaa gataagcagg
    22861 gagtgtaaaa tgaaaaaatt gattttatct cttactgcgg tggcaatgtt tctaccttta
    22921 atgcttgttg cccaggttta tagcattgaa ggatactggc atgacccgga taaaactgca
    22981 aaagtgcaga tttaccagcc ttatacagat tattattgtg gtaagattat ctggctaaaa
    23041 gaacctatgg aaaatgggaa acccaggatg gatatcaaaa atccagatcc caatcttcgt
    23101 tctcgtcctc tgatggattt ggttatcatc aaagggttag ttcctgcagg tgaaaataaa
    23161 tatgaaaatg gcaccttcta tgattatgcc acaggaaaca cctattcagc aaaagtagaa
    23221 ttaactgggc ctaacactat gaaactacgt aaatatatag gtgtatctat tatgggtcat
    23281 accgaaacct ggaccagagc aagataaagt agtttgagtt agcagaattg aataaatttt
    23341 ttaaggaaag agagtaaagg aatggttcat aagacagata aatacctttt aaccgatgtt
    23401 aatcacttaa gacgaattaa attcgctcaa ttcagaagtc aaattccagc tcttattatt
    23461 cttgttctgg ctattataat gaattctatt tttcctaata ataatcgttt atactacgat
    23521 gtggcactaa taattgctat tctggcaatt gcagcctaca ttattattgg actttgttat
    23581 cgttatcgtc agcgtatacc aattcctcca gagaatgcca tagtttcacc tgtgcaaggg
    23641 aaaatagcct atatcagaag aattgatgat aacactttaa taaatataag gaaaagtctt
    23701 tttgaccggg tggaatttcg tagtccacat tcctctgctt atttagaaaa cggggttgtc
    23761 caatttgaaa cacctgctgg aaaagcctac attcgcttga atttcaaaaa accttactgg
    23821 ttctcagaac ctgatttcac tacgggaaat cttatcggaa tggttaccgg ttcaggaagt
    23881 tgcactattt ctttacccgg caaagtatcc ttaaactttc aagctggaga taatatagat
    23941 gccggtgaac ccctgtggga agacatcaat tccctgaatt atgattcttc ctcttctcct
    24001 tatgaaagag tgaatttatg agtaatcaaa aatcctctcc catattaata ctccttctaa
    24061 taagcctcct gatatttagt tgcgtttttc ttaatggaaa atcagtgctt tcctatctgg
    24121 tggaagaagc cgaaataaat gaagcttctg gaatagcttc gagcctgaaa tatccgggat
    24181 tactttacac tcacaacgat tccggtggtg aagcagcagt ttatgtgctc aatcaaatag
    24241 ggcttatgcc agcaaagatt atcctgaaag gaattgaaaa ccgagattgg gaagatattg
    24301 ctacttgtta tgaccccaaa gacaaaaaac cttatgtgta tgtcggagat attggagata
    24361 ataattcatc gcattcttct tcctttattt atcgctttga agaaccagag attaaggata
    24421 ctttaatact tgtccataat atacagaaaa tagaatttgt ctatgaagat ggacctcgcg
    24481 atgcggaagc gctaatggtt gaccctaaaa caggagatat ctatattatc agtaaaagag
    24541 ataatccagc taaaatctat caactatctt atccacaatc ttttactgag gtcaatactg
    24601 cccgtctaat tggtactctt tcctataaat gggttgttgc agcagatttt tctcactccg
    24661 gagattatat tcttgtgaaa acttataatc aaatctaccg ctataaaaga agacccaata
    24721 aaacggtaat agaagcatta atgaccaaac ctaaaatctt gccttatgag ctggaagaac
    24781 agggtgaagc agttgctttt gacgctaagg ggaagggtta ttttactatc agtgaaaaag
    24841 cagagaatac ggaagttaaa ctttattact atagataatt ccagttcttt actatatatt
    24901 aaataatatc aattatatca tttaaattat ttatttatta atatattcag gggttttggg
    24961 ttctctctta gctcttcctt agctctatct ttttaacttc ctatccccgg agataaggag
    25021 cagacagggg aaagagaggg tggagatagg gattttgggt ctctgaaaaa attgtcttca
    25081 aaattaattt aatcaaatat cattatccca gttgaaatta atattttaga attccataaa
    25141 aatagtaact ggaccatcat ttactaatgc cacttccata tgagcgccaa aaacaccagt
    25201 ttgaacatta ataccagttt ctttaattga ttctgtaaat tggttgaaca gattttcagc
    25261 gagaacaggt ggagctgcag cggtaaaatc cggtcttctt ccgcgtctac aatcggcata
    25321 aagagtgaat tgtgggacaa gtaatagact gccacctata tctttaactg agaggttcat
    25381 tttattattc tcatcgtgaa agatacgtag ttccagcact ttctttttag ctaaaggtat
    25441 tttagcagcg tcatcatctt taccaaaacc gactaaaagt agaagtccgt ttttgatgtg
    25501 agccactata tgctgttcta ccagcacttt cgcttcgttt actcgctgga ttataattct
    25561 catcaatcaa ttttgaagtg atgcacttcc caacaaccag catcatcaag ccacactgaa
    25621 ccctgagtaa atggtgggat ttctatgata actctaccaa aagaaacacc gggacttaaa
    25681 aatcctgcgc taattgacat tttatcccat tcatttttgc tttgaatatt atctttaaac
    25741 tttttatcaa tagagccatc agccttaaaa gtgagcatac gcaaattaat ttgtggtcct
    25801 ttcggttctg aagaacaggt ataacaacgg acataatatc cgccataacg gttcacttta
    25861 aaggggtctg agatgatgct tactttgtaa ggagaagcat caattcgcaa ggagctacta
    25921 ccatgtaaag cgcgttcccc atcaataaat acgatatttg gtggagtttg ttcttggtca
    25981 gcaggttcaa ttaggacgga ccatcctttt aaagattccc tggggtccag tgaataagcg
    26041 ttaaatcctg gattagatag tagatttggt aagccttcag tatctttttc aaaatacata
    26101 agactggaat ggacaaagca agaagtcaaa gttaaagcca ttccaatgag taaaaatccg
    26161 aaaaataatc tttttttcat ttcatcaacc ctctctcatt tagatatttt aatacaaccc
    26221 ctactgccag ggtactaaaa agtaagttag agccaccgta actgataaaa ggcaaaggaa
    26281 ttcccgtggc tggaatgagt ccaatattca taccgatatt gataaaggtc tgaaataaaa
    26341 gataagcaaa aataccagca gcggcaatac gacgttcacg aactctgatt tctccaatat
    26401 cgtttataaa tctatagaag aagaggaaaa ataagccaat taagaccaga cttccgataa
    26461 agccaaattc ttctccaatc acactaaaga tgaaatcggt atgatgttca ggcaaaaaat
    26521 gcatattctt ctgagttcca ttgagccatc cctttccaaa gagggaacca ctacctacgg
    26581 caattttagc ctggataatc tgatatcctg agcctaaagg gtctgccata ggatttaaaa
    26641 aagacaaaat acgctgttgc tgataatctt ttagtccatt ccagaaaacg ggtgttataa
    26701 gtgccagaaa gacattaaga atggacgcaa tggagatagt aatccaggaa agtctggcac
    26761 gtaataatag atataccaat actataatcc agatgataat agctatccac cagaaagaag
    26821 tgatgatact aataactggt gaaatcagta gtaagatata gtaaaggggt aattcagcgg
    26881 caatcaacat tgccagtaaa ctgaagaaaa agactaaagt agaaccaaaa tcgggttcaa
    26941 ttaagataag taaagccggt aggacagtta atcccagtgc gataaatatc tgttttattt
    27001 ctgtcatatt atcttgtgag attgctctcg ctaccaccag aatggtcaga agttttgcac
    27061 tttctgaagg ttgataatta attcctaaga aagaaaacca gcgatgagca ccatttactg
    27121 gaggtgtaaa gaggaccaaa atcaaagcca gaatatttaa tatataagct ggcactatca
    27181 acaattcaaa tatagacatt ggaatcttta ggagtagata gactgccagt aaggatatca
    27241 ccgcaaaaat agcctgtttc caccagtaat tttgagtgtt tgtgtatcca ccaatggtag
    27301 ttgtagaagc agaaaagatt acaatacatc ccagagctat aagtgcaatc aaatagccag
    27361 ccagaacaaa atcaaatttc tttttattaa tcataatctt taaagtttaa ggattggatt
    27421 caggatgatt ttctgtcaac ggtaattctg gtgaaggtac ataattttct gtggtagagg
    27481 aagtttccat ctccatccca aattcagctt cttgctcttc tgcagttagg aattggggtg
    27541 gtataggtgc agttctctca attctttcaa tattgcccat ataataatta aaaattttat
    27601 tagctatagg agctgcaact gctccacctc caccggcatt ttctaagaag acagtaacca
    27661 caattcccgg tttatctgtc tcaataaaac cagtaaacca ggcatgagtt agttttccca
    27721 tataattttc agccgaaccg gttttaccat aggtggttgc acctggcaca ttaatagctc
    27781 tggcagttcc tcccggtgca tttgtaaccg cccataaacc ttcttttata atttgttgag
    27841 tgtttgcaga ccagggcatt cggtaacttt tcaatggttc tatttgttcc cggtttatac
    27901 gtgctgttcc catagttcta tttaacagat gaggttgcaa ccagagtcca cctcgtgcaa
    27961 tagcagcata gaaagtgttt atttccatag gagtacaaag aacttcaccc tgtccaatgg
    28021 caagattagc tttgtatcca ctcaatcctg taactcttcc tattcttttc ttgtaatatt
    28081 tggtatctgg gaaaaagcca cttcgttcgt taggtaaatc aattccagtt ttatctgtta
    28141 atccacaagc tctggtatgt gcataaactt catccagttc catatgatta atcaaatcat
    28201 agaaaaagac atcacaagaa accttgaatg catctactat gcttaaagaa ccatgtccag
    28261 ctgcaaacca gcaacgaaag aaacgatttc caatctgtag tccacctctg caagctgcta
    28321 aatgggtgtt tctatcaatc acaccttttt ctaagccaag tgttcccatc acaggtttaa
    28381 aaactgaacc cggaggataa gttgcattaa tgactctatc cagtaagggt ttagctggag
    28441 tgtttaaact tgcccagacc tctggtgtga ttttttgcat aaagagatta gggtcataat
    28501 ctggtttact tatataagct aaaatccctc cagtttttac atcggttaca ataatacaac
    28561 cttttgcacc ttgggggaag atttcattag cataactctg caattcatta tcaatagtta
    28621 aaatgaggct cagcccattt tgaggtggaa gaaatatatt atctttaaag agttctaaac
    28681 ttcttccctg agcatctacc tggataatct ctttaccatc tttacccctc aataagacct
    28741 cataatagcg ttccaatccg ctctttccaa tatatgcatt gagggagtaa tcctcatctt
    28801 tgtagcgttc atattccttt gcatctattc ttccgacata accacaaaaa tgatttggat
    28861 atagatagtt gcgggttgtg ccactacgaa aactaagctc ggggaaatca ctaaccttct
    28921 ctgaaagctt tataaccgtc tcataaggta tattatccag aattaatatc tcttcatagg
    28981 tcttaaaacg ctgttcaaaa accatcttgc gcaattcctc tgcatcaatg ttaaagttat
    29041 atgctaaaaa agaggaaaga gcattcatat cggctatttt accgctggtg agatataaat
    29101 tgtaagaagg gatattagta acgataggac gatatttact atcgtatatt tctcctcgcg
    29161 tggcaggaat acgacggata cgcacaaaat tgctttcggc aatgcgttga taatagcttc
    29221 cttttactac ctgtaatttg aaaagcatta ccaccaatat catacataaa ataattaaga
    29281 caccgcgaaa tatatggagg taataatttt tcattttaat catgcttcag ctcaatgcgt
    29341 aaatgagata tcagatatat ggtccaatat acaaccatac tcattgcaag attatagaag
    29401 aatgccaata ttatcgtact tatgagagaa gaggtaaagc tatagcttat accccagata
    29461 aaatagctga taagatggaa aacgatatta gccactaaaa gtgataaaat ctgagctacc
    29521 acacttttac tttcaaaagg ttttcggaac aggtcaataa cgatacccat aataataaaa
    29581 ataaaggtat acataccaaa tgtttcagga atagtggtat cgtataataa tccgattaaa
    29641 aatataatta ttaaagaaac attataatca cgagtccaga tgatgtatac aatccagggt
    29701 ataaaaatta atggaatcac cccagctatt tccagagcag gcataattag gacttgaaga
    29761 taaaatataa ttaatccaaa tataaatgtc caaatatgct tccagaacat ctcaatctat
    29821 ccgtctaata atacaaaagg gagtaagttc atcactttga ccttgcggat tatccttcat
    29881 cacctgctcc aaaaattgtt tggaaattcc cttacttgct ccaatcaccc agcaacctcc
    29941 tatatcattg atatccataa tggcaatggg ataacccaat tctgtagaaa gctcttgtgc
    30001 tactttttca ggatgaatag gtcctttaat aacggtatgg atataaggtt ctatggtagt
    30061 ggtttctgca gcatcaatta gtgcagcttg ttttcctgca agacgataaa aatccccact
    30121 tcttttgaaa aattttttag taaaacctcc aactacagca gcacaaaaaa tgcgtaatgc
    30181 accacactcg tctatggcac attgcatgct ggtgggagaa ccaagtccaa tgccataagg
    30241 tactcgcata actttagagg aaagaaagcg agctaatcta cttggcttta tattttccac
    30301 tggaacagct cttccttgtg ttattgcaag gggactttca ctaattgtca atatatctcc
    30361 ttgctgcata agaggtaagg catattctct gattacctga gcaatattgt cttctgaagt
    30421 taaaattctg gtctggacag ggatgcgttc tatcctcttt ccattatagt ttatgatagg
    30481 tgagatattt ttgttcttat tcataagata ttattcttct ttattataaa gatgtgttcc
    30541 agattctcaa cttgtgtaaa gggctgaatt tcagctgaga taaaaagata gtccttagat
    30601 tcatgtatac gagaaacaat tcccaccgga taacttttag gatacaaatc agaaagattg
    30661 gaggtaacaa ttgtatctcc cacacttatt tgagaaccta ttttaatcat attcatataa
    30721 attttgccat taatatcggc ttgcagtata ccctgcacgc cagtatcttt atccatcaca
    30781 ggtaattgaa aacgaggatt ggtgaaaggt aatactatag aatggtcaga agcaaccgtt
    30841 accactttgc ctacaatacc attggtagaa atgacaggaa gttcaggttt tatcccattt
    30901 agcgagcctt tgttcacaat taagttcctt tgctgaaacg gtcctgaata gccaataacc
    30961 tctgccagaa caagctccac ttgacccgta tcaaaggtta ttgattcacg ggtctgactt
    31021 tccataatga tatttctgag ttttagattt tccagcttcg tttctgttaa ttgcttactt
    31081 aattgctgaa cttccttttt taaggtagca ttagttttga tagattctaa ggaagaagaa
    31141 atggggaaaa atatggtttt actgaaaaaa gaggcacgat gaattctggt ttccgtgctt
    31201 cccatcatca ttatcaggga tagcaccact aaaataatgg gtagcacgaa tcttttcacc
    31261 tgttctatcc ttctttatat tcaattttat gactcaatat ctcaggggaa agttctattt
    31321 aagtgataaa agctgattca atcttctatt cgcttaatga gaacttctct gtaatggtct
    31381 atatcatcca ggacttttcc tgcaccttta accacgcatt ccagtgcgtc tggaactaca
    31441 taaacaggaa ggtctacggt tttagagatt ttttcatcta aaccttttaa caaagcaccc
    31501 ccacctgtga gatagattcc tcgttccgca atgtctgcag caagctctgg agcagtccgt
    31561 tcaaacaatc ttttgataga atccacaatg gtactaacag tttcagatag agcttcccgg
    31621 atttcttctg aagatatttt gatagtaacc ggaaaaccgg aaacaatatc ccttcctcgc
    31681 acttccatag tgagctcttc ttttagggga taagcacttc caatcgtggt ctttatcttc
    31741 tctgcagtaa gtaatccaac atgaagattg ttctttttcc ttaaatagtt gatgatatca
    31801 ttatccatct tatcaccacc aacacgaatg gaattatgga ctacaatgtg agaaagagaa
    31861 ataagcgcta tctcagtagt tccgccccca atatccataa tcatatttcc aaaagcttcc
    31921 tcaataggga ggtctgctcc tatagcagca gcaacgggct cagaaaccag ataaacttca
    31981 cgagctccag cgtgaagagc actatctctt acggctcttt tttctacctc tgttatgcca
    32041 gatggaacgc atacaataac ccgaggacgt actaataaac gcttcttttg ggctcgcaag
    32101 attaaatcgc gcaacatcaa ttctgtcact tggaaatctg caataactcc atctttcatc
    32161 ggtttaatga ctttcacttc atccggattc ttccccagca tcactttggc ttgttgacct
    32221 atggctataa ttctcttatt atcacttgat actgcaacca ctgatggttc attaatgacc
    32281 acaccagcat tctttttata caccaaggtg ttagctgttc ctaagtctat ggcaatatca
    32341 ttcgctttca ttcccagtat cccaaaaaat gacatttaaa cccctttatt tgaaaagtag
    32401 tggtaaacat tcacaattca cttaattcaa tattatttaa attactttga ttttaacttt
    32461 atctacgatg tcaaggaaaa agggtaattt agctgcattt gcgaaaataa aatccccttc
    32521 ttcttgttaa gttgggaaaa tctctttggt cattcatacc tcagtataac cttaatatct
    32581 gttaattaaa gctttaagat aaagacacta tgagattaaa atacgcttac ttctttccca
    32641 atttctgtaa atattgctgg atttcttccc gctccttatc ataacctggt tttcccatta
    32701 gggcataagt ggctttttta cctgcttcaa ctccaggttg gtccagtgga tttatatgca
    32761 atagttttcc ggtaaataca gtttgaattt catacatcat aatgaattct cccagatgga
    32821 attcatcaat ttgagggaag ataatatttg cattgggacg tttggctttt gttagggcaa
    32881 tttcagtggc aagtctttct gcattaagta attcagaaag tttatgttct cccagataac
    32941 taacatcttc actttcagga tggagattgg gaataacata atcatgctgg aaatttaaga
    33001 cagttaaaaa agttataacc ttatcataag gaccttccgt gtatagctga atttgagaat
    33061 gctggtcggt tgcacctaaa gccttaactg gagtttgacc aaccagaatt tctcgccctt
    33121 taaggtcata gcgtttacct aaactttctg cccaaagttg acggtaccaa tctgccaggt
    33181 cataaagtga attggaatag ggcatcatca cagctatgtt tttcccttct cggaaatata
    33241 gatagtgcaa tagtccattt aaataagcag gattatcgta tatattagtt aaatcagcac
    33301 aacgatttcg catagaagct gcaccttgta acagcttctg aatgtcaatt ttagcaaaag
    33361 cagaagaaac cagtcctaca tcactaagta ccgaaaaacg tccccctaca ttatcaggaa
    33421 ccacaaaaga gggatagtgt tcctcattac acagcttgcg taaaaatcct ttatccttat
    33481 ccgtggtaat gattaatcgg tttttataat cttcaggata gcgttttttg agtatatccg
    33541 taataatcaa ataagcagcc atagtttctg cagtagttcc acttttagaa ataacattaa
    33601 agatagtggt atcaagatta atttggttta aaatttcaaa gaggaaaacg gggtctacat
    33661 tatcataaac aaaaagcttt ttatctaaat tgcgttcagt tttaagagca gaatatagtg
    33721 ctttattacc cagagcagaa ccaccaatac ctaataccac catagtatca attttagggt
    33781 caagagattc cataaaattc agaatatggc taacatctcg ttcaggaagg tcataaaaac
    33841 ctaaaatacc agcttgacgg tcagcctgaa tttcttcatt agccagtttg caagcgcgag
    33901 cataatctct tatacgtgat tcagggatgg cggtaggaat tagttcagaa tgaagaagat
    33961 tacgatattc aaaagttaac ataatagctc ccttaaaatt tgtagtaagg ttttaattgt
    34021 tcaaagatta gattttctat ggaatctaaa tgctctaaat cagtgttttt tattaggtta
    34081 aaaagatttt catcacaacg atggataata tttttgcatc gtaaaaacgc agcagttcca
    34141 acactgagtt ctttaaatcc cataccaatt aataaaggaa catattgcgg ttgcgatgcc
    34201 atttcgccgc aaatagatac aggtttttca tatttattcc cagcttgtaa ggttagcatc
    34261 ataagtttca gtacagaagg atgatgcgtg atataataat ctgatagagc agaattattt
    34321 ctatctgcag ctaaggtata ttgcaccaaa tcattagtgc ctatactcat aaaatcacag
    34381 ttttctgcta attcttctgc acaaagtgca gccgaaggga tttctatcat aacgcctaaa
    34441 gggagatttt tagaaaaggg gatattctct tttgccagtt caattttgca ttcttccact
    34501 atttcttttg cctgcagaaa atctcggtaa tccttaacca tcggaaacat cagttttact
    34561 ttgccaaaaa tcgcagctct aaggacagct cttatttgag ttttaaagat atccttatga
    34621 cttaaagaaa aacgaatgcc ccgacttcct aaatagggat tatcctcagg atttgaaggt
    34681 atgagatggg aaagtttatc gccacccaga tcaaaagtgc gaattataac gctgtgaggt
    34741 gcaataattt ctgctacttg tttataaata gagaactggt aatcttcgga aggtaattca
    34801 tctttcccta agtataaaaa ttccgtgcga taaagtccca caccatctgc agctatttta
    34861 gagggttcat tcaaatcaga taaagggtcc agattgcaac gaagctttat tctgcgtcca
    34921 tctttagttt tgatagggga atccttttcc agaattacag gttcttctgg tttaggataa
    34981 tttgcaaata gtttttcata atattctaag gtggcactat caggttctat tatgacttta
    35041 ccgcttagac catccaagat aattttctca ttttccgaaa cctttttcca aagctcaggc
    35101 aaggcagtaa tagagataat ttttagagca cgactcagaa tagaagcatg agaagttaaa
    35161 ctaccctgtt cagaacaata ggcaggaact ttataataag caaaagcact aactaaggaa
    35221 ggtgaagcct cgtgtaaaat ggcaatctgg tctggagccc aattatgtaa aacttcttgt
    35281 tgttgtcctg taatttctag caaaagacgg tgttctacat cacgataatc agcttcacgc
    35341 tgtgccagga aatcgttaga taagttttta atgtgattct gcacctgaga aaagacttgt
    35401 tgaatagcaa aaggggcagg aactaagttt tgggagatat aatctttcac ttgagaaaca
    35461 agctcggggt cttgaagtat taagagatga gagtgcagga ttgcttgttc ttcttcagaa
    35521 tctttaagct cttccagctg aagcttgatt gtttttgcta aggaacttac tgcctcttca
    35581 aaggattgga tttctgtggt tagggaagaa gcagcaatat gagtagaagg caaatcccag
    35641 gatataggtt ccagttttct tgctcttccc ataatcaaac ctggatagat gaccaatcct
    35701 tctattatat gcataacttc actcttctcc aaagccactg gctattaatt ccgaaatttc
    35761 atccagaaga tattcttcgt ccactccatc tgcaatcaaa tccagaactg aaccacattc
    35821 tgctgccagc atcataactc ccataatgct tttaccattc acccgcattc catccttttc
    35881 aataaagaaa tcacttctat acttggttgc agctttcact aatagagctg ctggtctggc
    35941 atgtaaccca agtttgttta caactttgac ctttctccga ctcattcagt taagtgacaa
    36001 taggcttttg taatgtttgt ttacgaattt gttcatgtag aagacgatta aaatcctctg
    36061 cggcatcata accgactccc ttaagaatca tattcattgc agctacttct acaataacgg
    36121 aaacattttt tcctggcgat acgggaagat agataatagg aagtttcact ccgagatgtt
    36181 cagcatagtt attggctaaa ccaatgcgtt cgtaatcaat attctcctgc cagggcatca
    36241 attcaatctg caaatcaatc tctttttgct ttctcactct ttctatccca aacattcttt
    36301 ccacattgac aaatcccaca cctcgtattt ccataaaaga gcccaggtct ctgctggatt
    36361 ttcctattaa tttatcatca atcaaacgga tagtaatcaa atcatctcct accaaactgt
    36421 gtcctctatg aattaaatcc agagcacatt cacttttgcc aataccactt ttaccggtaa
    36481 gcaatattcc cagactgaac acttcaacta aggtagcatg gatagtcttt tccagagcaa
    36541 agacatccag caaataccta tttaaaagct gaataagctg agctgtggaa agacgactgg
    36601 aaaggatggg tatattgagg tcatttgcca gatattcaat ggaaggagga aaggttagac
    36661 ccttagtaat gattatgcaa ggtatgtcca tttgaaagat gcgacgcatt cgttccagca
    36721 taagctcttc aggtaaagtt tgtagatatc ggacttccac ttctccaaag acctgaagac
    36781 agtcagaaaa gaaaacatcc agatatcctg ccaaagctaa acctggccta ttaataaact
    36841 gtgagctgag ttttttattt aaagtctcag gctcagtaac cagtgaaaga gcaaggtctt
    36901 ttttcttagt ttcaaataat tccctgatgg tgataacttt cattcctatc ctcgggaggt
    36961 aagagagccg gagggaaata gaccttccgg ctttccaccg aagtatctta tggttctatc
    37021 aatttataat tctgctcatc ctttttaacc aggacattga gcttatctgt ttctacattc
    37081 ctgaatatca aaaagttttc cttttgacgg tccagtttat ccagagcttc ctgtaaagat
    37141 aaagtctctg ctacagttcg tttagtttta atggtcttac gggtgtgatt ttccggattt
    37201 acaatgatat cagattcatg agaatatgag ataaactgtt ctttcagggg cttctttttc
    37261 tgatggtcag ttatccggtc tttcaatttt ttaatttgaa cttcaagttt gtctacggca
    37321 gaatctatgg caagatacat atccatttct tctgcctggc tttttagaac atatcttgga
    37381 gcatgaagcg acaattcgca aatgttgcgg ttgtcttcta aagataaagt tgcatggatg
    37441 ttgattatat ggtcaaaata ccggttcaat ttttgacagg atgattccac ataatctctg
    37501 attgccttgg tcagttcaaa atgacgggca gtaatcgtga tttgcatgcg ttagtcctcc
    37561 ttactaaatc ttatgtatgg aaaggttatt caaatcctca agggactctt taattgtttc
    37621 tgaaagtgtt ggatgagcaa aagttagata atctatcacc tctgattttg ctcccagtga
    37681 aataagaata gctccttgcg ctataagttc tgtggcatta gcacctatga tatgcattcc
    37741 tactaattca gaattatcag cacgagcaat tgtcttcacc atcccataag tgctgctcat
    37801 agccatagct tttccattgg cactataggg aaacttaccg atttttagtt ctccaaattt
    37861 tttccgtgct tcttcttcag tatgaccaac agaagcgact tcaggactgg taaaggtgca
    37921 acgaggtatg tacgtataat ttaaaggtgg atgatgataa ggaatattta atatattact
    37981 ggcaatatga gcagcagcta tttctccttg tttactggca gtgtgagcaa gagccaattt
    38041 tcctgtaaca tctcctattg catatatatg atttagatta gttcgcataa attcatcaat
    38101 aacgatagca ttatgttcag tttccagttc catttgattc caatcaagat tacaattagg
    38161 gatgcgtcca acacttagca atactttatc taccgtaaga gttgagccat tagaaagaga
    38221 aagtttctgt ctctgttcaa tttctttaat attttccaca gcggtagaag tgtaaatctt
    38281 tatcccgact ttttttaatg ctaccgtaag tcttttggat atctcttcat cttcactggg
    38341 aagaatacga ggaagaaatt ctataataga ggtttgcact ccaaaactat tcattataga
    38401 ggcaaattca caaccaataa ccccacctcc aactatggct aaggattcag gtaaagttga
    38461 tagattgagc ataccagttg acgataagat attaacttca tctatggtta tattaggtaa
    38521 ggattttgga agtgaacctg tggcaatgac tatatattct gtttctattt gttcaccctt
    38581 agctgtctgg attaaatatt tgtcattctc taagctaatg gctatagctc tatcttttaa
    38641 aatgggaata ttgcgttttc ggaaaataag ctctattcca tttaccagtt gttctacgat
    38701 agcatttttt cgattgaata cctgtttata atccagttct ggtttatagg aagtgagacc
    38761 ataatttgaa gcagagctca tttcatagta tagctctgca cttttaacta atgcttttgt
    38821 tgggatgcaa ccgcgattta gacagacacc tccaactctt tcttcttcaa tcaggaggca
    38881 ttcaatccca tattgattta accttatggc tgtttcatat cctccgggtc ctccaccaat
    38941 tatagtaacc ttatattcag acaagagttt ctcctttaca tcctgcgtaa acgactattt
    39001 agaataccca gttcaaatct atatttagct acaattcttc gggaaataat tagcccttcc
    39061 tttttaagaa gattaactaa ttcctggtca ctgagagggt gtttcttatc ttcattctct
    39121 accatccggc aaataaaaga tttaatctgc tgacgagaaa tgttttcatt gcgagtatcg
    39181 attccagccg tgctggagaa aaagtccttc aaagcataaa ttccataagg agtttctgca
    39241 tatttatgtt ttaccactct ggatatagtg gattcattaa ctgaaagctc tcgtgctata
    39301 acagaatagg ttagaggttg aattatacct gtcccattat agaaaaaatc agattgatac
    39361 ttgatgattg ccttagtaac tcgttccaaa gtgcgggttc gcatatagat gctcttaatg
    39421 agaaatttgg cactatttat ccgttctctc acgaatttaa gagtttcttt atccaaaaaa
    39481 ccatgtgcca ttaacttaag ataatgtgga ctgatgatta tattagggct tatatggtca
    39541 ttgataatta cctcaaagtc atcatctatt ctttttatgg tgatatcggg aactatatag
    39601 gcagtattag gagtgagaat tcgcaaacct ggtttagggt caagtttagc taccacatct
    39661 cgccagaata agatacaatc ctcagaaacg ccataataag aggctatttt ttgaaaacga
    39721 cgatgaatta aattttcaaa ctggtcagtg cataaaccta ccaggatact attctggcgt
    39781 tcttcttcag ataattgagc taacaggcat tcactgatat tacgagcagt gataccttta
    39841 gggggtagtt gaaggactat attatgtaac tcatttgctc gttctgggga aatgttataa
    39901 tctctggcgg tcttttctaa attaaattcg ggagaaaggt atccataact atcgcagtta
    39961 tcaagcattt cagctataaa atctatctcg ttatcaggca aagatagagg atatagttgt
    40021 tgcataaatt tctctatgcc actttcttca taaattaata aaacttcacc tttatctttt
    40081 tccggtttag aagaagaacc ttcatagttt tgatgatatt cattccattg gtctaaaatt
    40141 tcagttagtt ccttagcttc acttaaggtc tgtccccatt caaatgaact atctgtatca
    40201 tccaggtttt tctgactttc ttcaggaaca aatgccatag gggtctcttc taaatcttct
    40261 tcttcttttt catcacggag ttccaacaat ggattgttaa ccagctcttg tttaatataa
    40321 ttttccaatt ccaggatggg aagagtgagc attttcaaag attgcagcat ctttggcttt
    40381 aaagcaagct cctgtttttc ctgcattgat atgttctgat taaaactatt cattgtcggg
    40441 actcaaaagg attagttaaa aacctatctc ctaaatataa ttttttagct ttttcatcat
    40501 ttatcagctc ccatgaggaa ccggatacta taatttttcc ttcaaatata atataagcac
    40561 ggtttactat tttcaaagtc tcaaatacat tgtggtcggt gaccattatt ccgatattct
    40621 tatctctcaa tttagcaata atatcctgaa tatctgccac ggtgataggg tcaacgccag
    40681 caaaaggttc atccataaag atgaatgttg gattagttag caaagagcga gtaatctcca
    40741 gtttacgtct ctcacctcca gaaagagtgt aagctttttg tttggcaaga ggggttagat
    40801 taagttctgc taaagcttct tccagttttc ttttttgctc ctgtttactt atcttcaaag
    40861 tctgcaatat agccagtata ttctcttcta cggtaagttt agaaaaaatt gaaggtgtct
    40921 gcgccaggta tcccagacca agtctggcac gacgatacat aggaagatgc gttatatctt
    40981 tattatccag cagaactttt ccttcattag gtttggttaa gccgagaatc atataaaagg
    41041 ttgtagtttt accagctcca ttaggaccga gaataccaac tacttctccc tgttgcattt
    41101 ccatactgag gtcgttgact accttgcgtt ttccgtatat ctttgccaag tgctgggctt
    41161 gaattttgtg aatttccata aattaaccct tagatgaatc tattctgtct gtcaagattt
    41221 gttttggaat ttgtagatac ccttgatgtt catattcatc ttcatataat caagtttatt
    41281 ctctggagta aactgaacct ccagataatc accagtggct gtgttatcaa agtaatcgtt
    41341 tttatctgtc ttttgacgaa tatagcgata ttgaactgct ctttcagcat tgaaagaacg
    41401 aatttggttc tcttcaaacc caatatcaat ataatctgct accacccagt taaatttagg
    41461 tcccatcgct tcttccgaaa attctactct acaggaatca acgagttcag ctctattcaa
    41521 ttttctttct ttaaaatata gataaaattc acccgcttca gcttttgcat aatcagaatg
    41581 gaattctggc attcccgtga agacagcttt atcttctttt aagaaataga gcagaaaatc
    41641 actgacggtt cggtaatcac gggtttgagc ctggacattg aaagttgcca ctaatttccg
    41701 ttctttttca aagtattcca tcttatcagc attcacataa agagtatctt cctgagctgt
    41761 gattatcctg ggtttctcaa tcatataagc ataaccttca tttctatccc agaaagcgta
    41821 accacaaaat atggaaacat tctcttcttt atcataggca gagacatttt cccatacagt
    41881 aatttgatta tatttttggt tatagatagc ataattggaa cgaaaccacc gaaaagcact
    41941 gcctttttgg ttctcttcaa tatagatttt tccacctgca ttaagttcat cgggaatgcg
    42001 atagtaggtt aaactatcag caaacagggt taaagaatca ttattaacct taacattacc
    42061 atccagacgg gctatctttt gcagattgaa aatagtggca cgatcactgt gaaattcagt
    42121 cttaccatag aagaaatgga ctttaccttc aagacgcatt acctgttctt cctgaacttt
    42181 agttagatat aatttatctg aatgaatcac tcgtaaaggc tctgattttt gtgcccagat
    42241 aggaagcaga attaaaagca gaacactacc aattaagaaa ttctttttct tcaaataaac
    42301 cttctgcgga gatactatcc atttcagttg gataaatact taaatttgtt cggagatttc
    42361 ttcctcgtaa tatatttcct gctctgacga gcataactgt gtccggtgca actatgacat
    42421 cgctatttcg gtcccaaatc atacgattgg tgtagatgct tccttcagct gaaattagct
    42481 taacattccc atgtgccata atcatatttc tggcttcatc tacaattgtg ctatctgctt
    42541 ttaaaaaaga agttccaccc gttttcttgt ccagagcctg aatctctact ttatatgcat
    42601 tcagaatgcg gcggtcgtaa taacgatcta tcctttccgc atttaaaata taagcgattt
    42661 tattttgttc atattcggta agagtcacat ttgtgctcgt ctcatctgga atcccacgtt
    42721 tcattgattc cgtttgaata tccagtttac tttttccaca agcactaagt gctaatagag
    42781 agcataaaag gattaactca actgatagaa ttaagatagg agagcaatgc ttgctcatag
    42841 attccttgat tggtaagcag tagttcaatg cattctctaa ctgctcctct tccaccatta
    42901 tgttgggtaa taatatcgca aaaagcttta acatcttcac aggtatcagc tggacaaaca
    42961 gatatggcag accttagcat caaaggaagg tcattaaaat catcgcccat catcagtaga
    43021 ttatcaaagt ccaatccaag ttcctgaagt aactgattaa ttttatccag ctttttcaag
    43081 actccctgga aaagaaagtc tatcttcagg tcctggcaac gacgctctaa aagaggtgaa
    43141 tttctgccgg taataacagc ggttttaata tcagtatgtt ttaaaatatt aaaacccata
    43201 ccatcatgag catcaaaatt tttactttca ttgccagcac tatcgtaaat aattcttcca
    43261 tcagtgagca caccatcgca atcaaagacc attagtttaa tctttgtcca ggctaaagaa
    43321 ggtttcaaag gtttttgtat attaataaaa ttcattcgtt atcctttaaa tgagctattt
    43381 ttaaacattc cttaatcaga ggagcaagca tatttaacct caacatactt gcggcatcac
    43441 tcaaagcttg ttctggctca ggatgtgttt ctataaaaag tccctgcact ttacctgtag
    43501 ctatggctgc ttttgccatc ataggtgcaa attctggatt gccacctgat accttaccag
    43561 ctgagggtaa ttgaagagaa tgcgttacat catagaccac aggatagccc aaagagtgca
    43621 taatgctgaa actgcgaaaa tctactacca gattatgata gccaaaacaa gttccccgtt
    43681 cagtgagcag tatttgttga ttatttccgg cttttagtgc agtttgttgc atatcttcag
    43741 gagccataaa ttgccccttt ttaatattga tgatttttcc tgtagcagct gtagcctgga
    43801 taagttcagt ttgacgacag agaaaagctg gaatttgcag gatatcacag atctgagctg
    43861 ccgcttcaac ttcaattctt tcatgaacat cggttaatac aggaagatgg aattcacttt
    43921 ttatatgttc cagagagcgt aaaccttctt cttcacccgg tcccatatag gaatcaactg
    43981 aacttctatt acctttttta taggaagctt taaataccac gataatgcca gtttcttctt
    44041 gcagttgaac taaagtttct gccactttat gcataactgt atcgttttct accacacaag
    44101 gacctgcaat taagaagaaa gctggtgtgg tcttaagctt ttgatataag tccattatcc
    44161 tgcctatttt aattctttga ccctttccca ggtaaagttt tcatcatcac gaccaaaatg
    44221 accataggaa gcagtaggga aaaatatagg ttttcttaaa tcaagatact ctattatccc
    44281 ttttactgtc aagggaaaac tcttacgaat aagggattcc agttcttttt cactcatctt
    44341 accggttccg aaagtatcca ccatcaaaga tacaggttcg gcttcaccaa ttataaaact
    44401 aaattgtagc aaacattctt ctgctaaacc agaggcgaca atactttttg ctatatgtct
    44461 tgccatatag gaagcgctac ggtcaacttt ggtggcatct ttacctgaaa aggctccacc
    44521 tccatgttgt gcccatccac cataagtatc aactatgatt tttcttccag taagtcctgt
    44581 atctgcagca ggaccaccgt cttcccattc attcaaagga ttaattttca cctgataatt
    44641 attggcttga aaagtgctgc aagtatcgtt ttccatttct tctatggtag gtttaataac
    44701 cagttgattt atggctcgtt tcatttcttc aaattctgat ttgctgatat gtttatgatg
    44761 agttgagata acgacagttt ccactgagac gggttttcta ccacaaaaac gcatactgac
    44821 ctgggattta gcatcaggta ggagataggg gatagttcca tttttgcgaa gctttgccag
    44881 attttgtaat agcttatgtg ccattgcaat tggtgcaggc atatagtgtc tggtttgggt
    44941 gcaagcataa ccgaacatca aaccctgatc gcctgctcct tcattttctc ttaatgctgc
    45001 ttcctgggaa tggagatagt tattgatctc acaattataa tcaaaaccaa taccaggatg
    45061 gatatagcca atgtttctaa ttacattacg tacaataggt tcaatatcca gatttaagtt
    45121 accaggtgaa gatatttctc ctgaaagaat tactctattt tcagtgcata gcgtctcaca
    45181 agctactcgc gagctgggat gctctgttaa ataagcatcc agtatagcat ctgatatctg
    45241 gtcacagacc ttatctggat gaccttctga cactgattca gaagtaaaca catactcatt
    45301 tttctcacac ggtttgttct catgtaaggc tgtcattcat ttctccttta ttaacttaca
    45361 gaatatattt cctgtttcaa gatgcagcta tgcttcactt tgtttttatg tataaaaaaa
    45421 atccggctgc taaagccgga taaaatgccg cactttagca cattttgccg cggagcaaag
    45481 ggcaaatcac cgcatctaaa aattttggta catctccggg gattcgaacc ccggacccac
    45541 tgattaagag tcagttgctc taccagctga gctagagatg catttctata catataaatt
    45601 tttataatcc cctttgtcaa gcaaaaaaac tccatttttt gatgacctaa actgtagtct
    45661 aagaatccga taattgcttc attttaggtt ttttagcttg ttattaattt gtaaacatta
    45721 accttctaat aatagggaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    45781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
    45841 aggtttttta gcttgttatt aatttgtaaa cattaacctt ctaataatag ggaaaccgat
    45901 gtccaactta tgtccagctc atgtccagct catgtccagc ttatgtccga aatgggacat
    45961 aagttacctg ataacattac ataatgtcaa ctttaagaat tgaggataag aaatagcata
    46021 aatgtataat tgggaaaatc cttaacttat tgattagcaa tgaagttact taaataaatg
    46081 agagaagaaa gaggtgacaa aagttttgaa tttaatgatt ttatccttgt tttttcttat
    46141 tgacaattag ataatgagct ttgaaattgt attaattgga aaagtcttat gaaattatct
    46201 atcattatag ctgattgtat tgaagctcaa aaacttaata gatttaagtg gttgttgcaa
    46261 agtaattgtg aaaaggatat tgttgtatga aatcaagatt tagtctattt aaggaatgaa
    46321 aatggaagag aaagagaaaa aaggcttttt ccaaactatc tggttgctta taattggtat
    46381 attcaagacc gcaaaatatg atatagcagt attctggaaa tatataagta attctcaatg
    46441 gcgaaaacga ttatgggcag atttaactaa tttcgtcaaa tcattctatc aaattcttat
    46501 gaaagaaaga gtattaagag aagcaggagc gctaacttat ataactatta tgggatttgt
    46561 accagtagtg gtgttcctta ttttccttgc tcctactcta cctttcctgg atttaaaaga
    46621 gaaaatattc tctttaatct cagaaaatct tataccaagt gcagctttac aggtgcagaa
    46681 tataattcag aatatgttgg aaaataaagc aggattcaat atcatcaact ttatagttat
    46741 agcagtttcc tcatattcct tatttatagt tatcagggat tcttttgacc ggatactgag
    46801 acatgaattc agaactaaac cagggttaat tagtcaaatc ataaagtttt taggtacatt
    46861 gattctggga ttgcttatta tgatagtgct tttatcaacc tcgtctattc caattatatc
    46921 tggaatttta aaacttcctg tattcaaatg gtttagtttt ttaatacctt ttttgatgca
    46981 atatattgtt ttattattac tatatagttt gatgccttca gtaacggtta aagcaacttc
    47041 attggttaga ggtgcttttt gggctgcaat tatatggcta attgccagaa gtggatttga
    47101 tttctatatc tataacttaa ccacctatcc tgaaaggtat ggtgcagtct ctgctttacc
    47161 tatctttttg ctctggatat atgtgaactg ggtcattata atgagtggaa tggtgatggt
    47221 ttcagtatat gaaaacagga aaaatggtaa agaaccaaca gagagtcctc aacagatggt
    47281 tagagttacg gttgaaatgt atactaatag taaattaaat aagcgcatag aaaaactctt
    47341 agccccagag gaattgaaga atatagttca acaagtagat aatgatgagg ataaatgaat
    47401 aaatatgctt tgctttccgt ttcggaccgt cggaacattg aaaaacttgc tgctgccttg
    47461 aatgaactgg gttatgctat tttagcaacg aaggggacat ccaattatct ggataatata
    47521 gggattaata acatagaaat tgctcggtta acacggtttc ctgagattct caatggaaga
    47581 gtgaaaacgc ttcatccccg tattttcgct ggaattctgg cagatagaga aaatttagaa
    47641 catatgaaaa ctctggaaga acttggaatt gattgtatag atttagttgc agtaaatctg
    47701 tatccttttg ctgaaacctg gtctcgggaa ggagtaactc attccgaact aattgaaaat
    47761 attgatattg gaggaccttc tttaatccgt gcagctgcaa aaaactacaa gagtgtaatg
    47821 gtcttaaccg atcctcaaga ttatgaatct gtcttggcat atttgcagaa taataaagaa
    47881 gttcctttgg aattgagaat tcatctggct cataaggcat ttgagtatgt gtcccattat
    47941 gatacaataa tagcagaata ttttaaatcg ctggattctt ttaaagaaaa ctcaccgaat
    48001 ataccttcat attatagtat atcttgtaag ctggaacagc ctttgcggta tggagaaaat
    48061 ccacatcaaa tggctggttt ttatctttct catcaatctc cattagaggt tttatcaggt
    48121 aaggaactat cttttaataa tcatcaagat attgatgcta ctcttagagg tttgagatta
    48181 tttgaacaac cagcagttat tattaccaaa cattgtaatc cctgcggaat tggtgtagat
    48241 tctgatttgg ctcttgccta tgaaaaagct tataaaagcg ataccgattc tccatatggt
    48301 ggaatcgtag gagtaaatcg ttctctggat ataaagacag ctgaattaat aaatcgggtc
    48361 tttacagaaa tcataatagc acctgattat gaaaccggag tcctggaatt tcttaaaaag
    48421 aaaaagaata ggagagtcat taaatataat ccagaactct taaacaaatt ttctgaaccc
    48481 tgggaattga aatgcttaac ctatggatgg ttgcttcaat cctgggattt ggtacaggaa
    48541 aatcctgaag aatggaaagt agtttcagaa caaaaaccag aaaaggatga atgggaagcc
    48601 cttatattcg cctggaaagt tgtctcctta cttcgttcca atgctattgc cattacaggt
    48661 aaagaccagg tttatgggct aggtgcagga caaaccagca ggattgattc tactcatctt
    48721 gctctctgga aagcacaacg ctatggacat aatcttaagg gtgcggtctg tgcttcagat
    48781 ggattctttc cttttcgtga ttcagtagat gaattacata aatatggaat taaagcaatc
    48841 atagagccag gtggttctaa atcggataat gaagttattg aagcctgtaa tgaatatggg
    48901 ataaccttag tttttacggg ttatcgtcat ttcaaacatt gatttttatt tgacagaaag
    48961 gatggcaaaa aaaattatat caccgaaagc ggagatgtgt ccgagctggc tgaaggagca
    49021 cgattggaaa tcgtgtatat gtgcacaaac gcatatcctg ggttcgaatc ccagcatctc
    49081 cgccagaatt ttttatagat atcccaattt tttggtaaat agagaggagc tttatagatg
    49141 aaaaaaaata gcggaattta tattggatgt ggtgtcattg ttttaattct ggcagttgca
    49201 tttttagccg gatattttat tgggtttgct ccccaaggaa aaaatgttgc ccaaggatcc
    49261 tggcttgttc tggatttaag tggacaggtt acggattatt ctatccttca gagttccaat
    49321 ttcctgggat ataatccctt aagtgttaca gatatctgtt ataggatacg aaaagctgca
    49381 gacgataaca aaatcaaggg catcctgatt aaacctaatt tgctacaaac taattatgct
    49441 aatctaaatg aaatagatgc agcattgcaa gattttaaga agagtcataa acctgtgatt
    49501 gctcatggtg aagtccttgc tcaaaaagat tatcttcttt gtgcgatggc agataaaatc
    49561 tttatggacc cttctgcttc tgctggaatc gtgctggaag gtgttgccag taatgttctc
    49621 ttttataaag atgcgcttaa gaaacttggc ataaagatgc atgtaatgca ggaaggtgaa
    49681 tttaaaggag cagcagaacc ctatactcaa aactctttaa gtccaggcac agaagaaaat
    49741 ttaaggaaag ttctaaaaag tcgctatgaa cttatacaac aagatatatc aaaaatgcgc
    49801 agtatgaaaa tagatacagt gcaaaatatc tatgaaaaca gaccagattt aaccattgac
    49861 gctcaggaag ctataaaata tggtttaatt gatgaagctg ccacctggga agaactccag
    49921 aaaaaatatg atattacaga taaataccag atttctatct ctgattataa atctggcaca
    49981 gaagcttcta tctcttcaga caaaattgcc gtggtttatc ttagcggtaa tattactcct
    50041 tcaattaatt ataataatcc caatgtgata agtttaaata gtatagatag gattttggat
    50101 aaattagctg atgataagac agttaaagca gtcgttttac gaattaatag tccaggaggt
    50161 tctgctcttg aatcagaatt gatttatcag cgaatcaaag cttttgaaaa accagtggtt
    50221 gtatctatgg gaggaatggc tgcatctgga ggatattata taagttgtgc tggtgattat
    50281 ataattgcag acccccagac aattacaggt tctataggag tgatgatggc tcttcctgaa
    50341 gcagaagagc tgggtaataa gataggtata gaaagtcaaa ctatcagcta tgggaaattt
    50401 gctggagcag gtaatatttt agaaaagact gatcctgaac tcctggcttc tttgaaaaga
    50461 gaatctgcca gcgtatatgc cgaatttaaa caaagagtga tggacagtcg taaaattggt
    50521 gctgataaaa ttgatttagt agcagaagga agagtctttt ctgcttctga tgctaaaggt
    50581 aatggtctaa ttgatgatat aggcaatctt cagtttgcaa taactaaggc tgctgacttg
    50641 gcaaaaatta ctaaatatgg tgttgaatca tatcctaaaa aggttactat atgggatttg
    50701 ttccgtcaag gtaattggtt acagaccagt tcagctaaag ctataaataa agaatccaat
    50761 gctattgaac cgatagaaga ttatctaaaa aagaccttac cgaccaatca atggctatat
    50821 ttttgtccct ataagctgga ttaatgaaat atgaatcaat caagaagcca caaaacagaa
    50881 catagttatt ctaaaagaga taaatacata accgaaatca tcgttaatgt gcatcctctg
    50941 gaaaagagag tggcagtttt agaagatggc agattagtag aactctttgt agaacgaaaa
    51001 gataaacaga atattgtggg aaatatttat aaaggtattg taaaggatgt gcttccggga
    51061 atgggggctg cctttattga tataggtctg gaaaggacag cttttttaca ttattcagat
    51121 atagttatgg attttcttga tatttatgaa aatgaaactc cccatcccag acttaaccct
    51181 aaggattctt ctcagattgg caatctactt aaaccaggtc aggaaattgt agtacaggtt
    51241 cataaaggac ctcttggttc taaaggagca agacttacag gtcaaatttc tgtcccaggt
    51301 aaatacttag tgctctttcc caataaacat aagattgcta tctctcgtaa aatctacaat
    51361 ccgaatgaac gttatcgtat ccgaaatatt atcagcaact ttaaagatcc caattttggc
    51421 ttaatagtta gaacggaagc tgaaggttgt gaagaagatg aattacgcac cgaatatgaa
    51481 gctcttttaa agacttggaa actgattgaa aagaaaatac gctatcataa agcaccggtc
    51541 tgtgtttttg aagaaaatgc tctggagaat tatctgatta gagacctttt tggcgagaat
    51601 gttgaccgtc tggtgattga tgataaagcc ttcgccaaaa gtataatcca tcaaatggaa
    51661 gatatatctc cagaattgat tcataatata gagatataca aagaagatac ccctatcttt
    51721 gatgcatggg gtattgaaaa aaagattgaa accatattcc attcacgaat atatcttccc
    51781 agtggaggaa atattaagat agagcaaact gaagctctgg tcgctgtaga tattaatact
    51841 ggtagcttta ctggaaagac aaattatgag gaaaccatta aaaagaccaa cctggaagct
    51901 gcagcagaaa tagcacgtca aataagactt cgggacttaa gtggcgtaat tattatagat
    51961 tttattgata tggcaaatga acaaaataaa caagaagttc tggaaatgct aagaaaaaac
    52021 ctgagaagag accgagctaa aaacaaagtc tataatttta ctgaattggg tctggtggaa
    52081 atgacacgga aaagaatgag aacttcgctt atcgctaatt tttcagatcc ctgtccactc
    52141 tgtcatggaa gtggacgcat tgtttccaaa gacactatgt taatgcgtat ttatagatgg
    52201 ttaaatcgct cagaatattt tattaaagat aggaaattgc gtatcattgt tcatcctgag
    52261 ttattagaac atatacagaa taattacgaa tattttaccg cttacaagga ccaaatagaa
    52321 atagtttccg acccagaaat gcgtactgac cagtttaaag ttctactttt gcccgattta
    52381 gaagatataa ccgccaaatt cagctaatat atgagcttaa tcttaacctt tattttatga
    52441 ttgctatttt acccctggca atattgcccc ccatttctac tacatagaaa taaataccgg
    52501 aagagactaa ctttccatca ttgttcttta aatcccatat ccaatctata ttattaattt
    52561 ctggattaaa aggacctatg gcagaactac gaacaagatt acctgaacta tcataaattt
    52621 ttatatgacc tctcttacct gcagggaaat taaggatgtg acattgtaag tcattaaatc
    52681 ttacgggatt gggataaact accagattct ttaaatcatg attattactc atataaaaac
    52741 gagtggtaga atattgtggt ataataggat tacctgccaa gtccctaaca ttacgcacca
    52801 ctatttgata gggtgaggaa ccaaatttta atttatgaga cagagagacc tgaacccggt
    52861 catctaaatg agaaactgat actatactgt tatccgggtc attggaatga cagattagag
    52921 tatagttatg caattgttcg ggactgggat tgtccggagc taatgattcg ctgaaagtaa
    52981 tttccacggt cttttggtca tttaaaacta ctgctccggt aaccttaggt ggttcagtat
    53041 cagcaatcca atagaattca tattgatttt cagccagagg aatattataa gcactaagca
    53101 tagttcccag agtaagtaaa aagggttcac ttgtttcagg aaatatctgg ctgaattgca
    53161 gctgaactcc atagtaataa ttgatataat tcactgaaga aggatgcgac attcctttat
    53221 ctactgtgta aagagaagga ttacagatag aagttgccat tggttgatca aacattacac
    53281 gcaatctatt aggacctatc atagtaacac tttcaatatg gggaacagga tagggaataa
    53341 cggattgcca gatagttgga gcactttctg aaggtgtaaa attagaatta taagcggtaa
    53401 tagcatattg ataggtgcga ccttcttcca catttaaatc aatataggaa ttccctgtaa
    53461 cattatccaa aatatatatc tgattgtttt cttcttttcg gtagattcta taataatcag
    53521 caccattgtc tatccaactt aattggactg aggattcatt tagaggtctg gcaataaaat
    53581 taatgggggt tggtggtcct gtatagggtg catcttgagt ccatacaacg gaaataaggg
    53641 agtccttttc aacttcataa tttgttaaaa agaacgattg attaggatga tctatataag
    53701 caagaataga ataggtattg taactattcc cataaaattg aggctggaat ttatcatctt
    53761 gatatttaac tatgtataaa ttaggtgaga tggctaaaat aatttcatct ttcccatcat
    53821 tatccaggtc cgctgcttga atagcatttt tagaagccac ttgattgaac attatggagc
    53881 ccatagaagt atagttatta ttggacacat ttttaaaacc ttcaaaatac caaaaattga
    53941 ggttggggtc aagtttatcc gtataatatc cacctatgaa gaaatcctgc cttccattgc
    54001 catcaaaatc gccactggta agataataag tgtttccaac aggcatcttt tgggtccagg
    54061 ttagttctgc aaaattatcg ttgcgaatct cataaatcat tacatcacca tccgtatcag
    54121 ctactaagat atccggatag ctatcattat caaagttctt aacaatgata gtggggacaa
    54181 aagcattgta agatgtagtt gaactggtat tatgcaaagt atttttaggg acaaagatat
    54241 ttcctgatcg tttatatgcc tgaataactc tttctgtggg aagagtttta accaatagaa
    54301 gatcatctat accatcatta gaataatcag ctatggtgcc tccatctata gaacggtctt
    54361 cccaaagggc aatattagga tacgaactat tttgactttc taagacggtg gctgtgccat
    54421 attttaaggt taaaagttct tgtccaatat tgttagtatt tcctgcacca aggagccaga
    54481 aagaatcacc aaaagaatgg cttactatat gtcctccagc ttcaggttga taagcataaa
    54541 ccggtccata acctgttttg ggtaaatcca tagcaatata ttcttttata ccatcaccat
    54601 tataatcata gctataattc aggggaactt ttggtcctcc taaaggacta tagatatacc
    54661 cataatttgg tatgagctca taatgaatat taagaaaacc attataccaa tcagatagga
    54721 aggttaagtt acttacattg gtagctttaa cctgaatgtc tatttgccct tcaggtagag
    54781 tttcaggtaa agaccatatt tgtaaagtat ccagcatagc tccatagcta tgataatgag
    54841 ttccgtttga agctattata tcaagttcag aacgcactgg ttcattaaat actgcaataa
    54901 tataatatct taagttttgt ttatcataac gggtgaattg ttgcaaggag ttttctttca
    54961 tttggggagg tgaagggtca taaataactg tccggaaaaa gttatattta cttccgttta
    55021 ttgcctcata ttgcacacgg ataatatact ctccttcagc aaatgattct ggtatataaa
    55081 aatgagctaa aactccattt tgtacaggtt gggtgtggaa ttttggatag ttatgatggg
    55141 tttccacatc ataccaatca agaagcgaag gaacagattt gttggaatac ataacagaat
    55201 agcggaagaa attgttacct tggactgtgc ctatgatatc aaagctttct gttaaaccgc
    55261 tctggtccag aggagaatca atataaacaa gcggtggtgc tgtgttttcc agaagctttt
    55321 tggtatttaa taagccatgt ccataatatt gatcaaagcc cggggtacct aaatcatcag
    55381 tggcagttaa aagacgtgaa cgaacttcac ttggactgag tccaggatgt aatgataata
    55441 ataaagcagc tgcacctgaa acaaaggggg cagacataga ggttccgctt tgttcataat
    55501 actgattccc cgggtcaagt ttataagtac tcaacaccat ttcaccagga gcaacgaggt
    55561 ctaaatcagg tccataactg gaaaatccag ccagagtgcg tgttctattg atagcaccaa
    55621 cggagatagc agtggaaagt ttagctggat agcttatata aggtccagga tcatttcctg
    55681 cggatgctac taaagttact cctctggaat aggcataagc acaagcatct cctattatcg
    55741 gagagtaatt agggtcaccc cagctcatat tgataacatt acatccatta tcagcagcat
    55801 aaattagagc agctgcagca tcgtcatcct gtaaatagcc ctgaccatca actgtcagga
    55861 aacctgatct aactatcatc aatttaatat tccagttaac tccacagatt cctactccat
    55921 tatttcccac agctccaata attccagcta cggcagtgcc atgaaagttt tcatcttcca
    55981 cattattatc aggattaagg taatctccaa tagcaatatc agctaattca ggagcatcaa
    56041 cgaaatccca gccataaata tcatcaatat aaccattatt atcatcatct ataccgttat
    56101 cgggaatctc tcttgtgttt acccagatat tggcagcaag gtcaggatgg ttaattaaac
    56161 atcctgaatc aacaattcca acgaccactg tagaactgcc cgtgcaataa tcccaggctt
    56221 gaggattaga aattatggta tggtattgac gaggaaagag ggggtcattt ggtatggtgt
    56281 gaaaagtaga taaatagttt ggttgaatat actcaattcc agcaaacttt atcttacctt
    56341 cttttaaatc ttgccagttt ggttcttgtt caagattagc aaggaaatac tggttattag
    56401 gcattcctct aattggtctt aaattatgtg ctcctaaagt gctgagccag ttgtcaaaag
    56461 aagttatacc agtttggtca gattttatat tcagtggctg actggttttg aaaattagct
    56521 gtcccggagt atattccaga gcagataaaa cagttaaact gattaaaaag aaaaagagga
    56581 agggaatata gcattttcgc ataagtatgc cacttaaaag tgataggaaa gaccaaaaga
    56641 atgaacattg ttcagatggt cagaaaaaga accccagcca taatctatac taaggttttt
    56701 ccagttcaat cccagaccag cagtaagatt ttctgcatca taattgattt tataaccaat
    56761 tctaagagcc aggagtcttt gaaataattc aatttcggaa cttactgcgg ctttccaatt
    56821 ttcatccact gcttttattc cactgagttc aaatgtaata tctgtatttt gataatttat
    56881 acttttactc aagtccatct ctaaacttgg agcaaaaagt gtgcgctctt catccatagc
    56941 actggaaatg cctaaattac ggacagataa attaaattgt gttcctgcaa ttggagttaa
    57001 ccaggtaaca ccgaggtcag tatgtagtcc gagactggaa tcagtatcca gtttttcata
    57061 agctattccc acattcacac cagcatatat agagggggtt aatcgcttag cataattacc
    57121 aagaaggtct atatctaagg gagaataatg tcctatcaac attccattgt catcgcggag
    57181 ttctaattcg ccataatcca ggctgcgaaa tgctaatcca aaatgactct tacgattaga
    57241 ccaggaatag taaagattat cacaggaagt atcagctaac cagatgatat ggcttgcacc
    57301 aatgctttta tgagcttcta tggttgacga ggcaggttga cgcagaaaag aagctaattc
    57361 cgaagagcta cttattcctc taccagccag agaaagtgaa attggattgg tgctaatatc
    57421 caagaattga tagccatatt ttcctgcatt atgatgtata tccgcattaa ggaatacgga
    57481 aaatagcatt atcagtgaaa gtattaaagc tactttatgc acagaaaact ccttcatttt
    57541 tccaccgcaa atttaatttg tttagtatct ctatatccag aaatcactgc gatatagacc
    57601 ccattatgca acctatcttc ggggaaatca atcaattcac gattacgaag atatgctcgg
    57661 gcataagctt tttgagagta gaccaggctt ccattaatgt caaagatttt gatttctata
    57721 ggggtgtcct gactggtcat tacattgagg gtaagtttat taccaaaaag gggtttcaag
    57781 ggattaggat aaatatagac ttcacctgga acaaagagct cagtggttag aaactgattt
    57841 gtgagaaatg aatcatcacg actggagtgt ctttgcagat ttccatattc tgttaaccat
    57901 ccactggaga agttttccaa gaccgcatca ggaagtaagg tacgataaat agttccatta
    57961 tcggaagcca cccaagcata gatatttcca tcagaagcct taccaaagaa aggtaaatta
    58021 cgactacgat taccaaaaga aacaggaaat ccttttttga cccgaaaatt atcttcccaa
    58081 catatcaatc tattattgct aaaagaacca agtaattcat tcttcccatc attatctaaa
    58141 tctaacacca ctgctcctga gttaaaagct aagctatcag aagcaggtaa aaagactgat
    58201 gtcggactca ttcgggaacc tgaatggtct ataatagcga acccttcatc tgaagtaatt
    58261 atcaaatcca aactaccatt tatgtcccaa tcggcaatag ttaaaggagc ggtagtatgc
    58321 atattatgga tgtaggggaa accgggtagc aaatttaatt cattatcaaa gacatagata
    58381 gaattagtaa attgaacaat cagttcacct gctctattta tgcttttagg ttgcaggggt
    58441 gccttgaaaa tggctactaa ggtggaatct acaggaagat ttaattgttt ttcttcccat
    58501 tcagtattaa ttaaatcata attccagata gaataagagc ttcctttttt gttcaagaca
    58561 taaaggttgt tcctgaaaag aatcatatta gcgaccacgg gaaagtcaaa ttgtgtcatt
    58621 tgttgaagct catcttctat cttattatag ctaaatatat aacttttagt gttatcatta
    58681 tcattgaggg caaaataaag cttatctccg tcggaaacag gatgagttgc ccaattgcat
    58741 ccaggaaagt ttcctataaa tcttttatca ctattactta gtttatagag tcgggctaaa
    58801 ctttgatatt gcattggaag gtaaaatgaa gaaccatccc aaacatagtt ttgggttacc
    58861 ggttgcaatg caaggggaaa acctggcata agagtttcat ttttccacat ataaagcaag
    58921 ccattgggca taggatagaa aatctcagtt tctgaatcac catcaaaatc taccatacaa
    58981 gcattgatag gattatcacc ttcataatta gcataaagtt tccagccaaa gctaacggaa
    59041 aaatacataa cattgccact aatacttata tcataaactt caagtgggac accaccataa
    59101 tagctttctg ctgctggtaa agaaagcaat ccattatgga atctattgcc aaaataatca
    59161 ttgttaccat tccggaaact atcatagggg ctgccatatt tataaatgct gtaattagca
    59221 gtatccagat gctctattcc atcagcttct tctaaatcca cacctttatg agctgaatta
    59281 gcatttacgc tgttcaaatc aaaattaggt gtgaagtttt gagctattac attttcatct
    59341 atgtgccaga ttagcaatcc cgaaccatcc acaggaatgt tcatccctga aggaataggt
    59401 cctcctaaac ctggtaacat aaaatcccat tcactgcctt tatatctatt tttcataaaa
    59461 ttgaaatagg gaagaagagg ataattttcg taataatcct gctcaccttc tggaaggaga
    59521 gggaaagaat aactgggaag tccagtataa gggtcagtgc ttccatcggg attttgttgt
    59581 ctattctcta tcaggaaata ttctctgctg gatataggga gtttgtacaa tctatttgca
    59641 gaaggattgt gattcaaaaa ataatctaca ggaacatttt cagtatcagt cgtgattgta
    59701 attacaggtt cccaatctaa gtataatctg caccagggat caagttgagc tggaacataa
    59761 ccatttgcat tccagatacc cgtacccatt aaaccccagt ttcctatacc ttgggaagct
    59821 ccattagagg aatcagtatc aaaaagagaa ggtaaaccta aaatatgtcc aaattgatgt
    59881 gcaagaaccc cataaatgct aaaaatatat gattcagcat taggttgtcc ttctgctggg
    59941 aaataatctt gatattcatt ctccggcact atcacaatgt tctttaaata tacaccatca
    60001 gctaaccagc cttgataatt gtcattatcg gggtcaaacc aggattgcag tcgcttacga
    60061 gttaaaaagg tagaccagat ttctccagtt cttattccat ttatgtctga ttcctgtcca
    60121 gctcctgcat gaaaaattat aagtccatca taaagactga agtctatttc tggatcagcc
    60181 atctgtaaaa gtgtctgagt aaattcattt aagtttacat caatattatc tgcagtatct
    60241 cttccatagt aagacatatt ttcgggtaaa gtaaatactg aaggatggag agtgtaattt
    60301 agctcataat ttccatgaga agcatcagca taaaaatcgc ttaaatggag catccagcga
    60361 tcaaaaaaag catcatcatg tgtcagatta tcgggccagg taggattatt tataaaatga
    60421 acatcctgaa attgcgctct aagaaccaaa atattatgcg gtaaggtcct gaatgctaat
    60481 ttattagggg aggtaaaatt tgtaggtaat tcaatttttg tgggagtata agcagcaggt
    60541 atatccttat ataacggatg aggtgggacc atagcttcta cataaacgga taaggtaata
    60601 accaggacca ggcaaataat tcttatgggt tgaaagtgca tagttggact tcctgctaat
    60661 aatctataaa gaaaaaaacc cgctaaaatt cagttaaaca ctattcttac atttgattct
    60721 ttaaaagaaa tcaatgattt ttcgtcaaca aaaaacccgt tctaccatgt agatgtaaga
    60781 tagtaacggg tttgctcaaa agggcatttt tttaattaaa gcttttttcc tgctaacagg
    60841 tcgttctcat tgcacttgca gactctgata tactcatatt ttggagacca ggaaccacta
    60901 ccttttttct tgttagttac cattttaagc ttttttaatt cagtgttaca aactgggcag
    60961 ataacctttc cttcactgga agtctcatgt gccagttttg ctgcaaaaga ttttgctttt
    61021 cccatttttt tcctctttta ccctgaaatt ttagggctta tatctttatt ttaattcgct
    61081 ctttcaatat agctgagagt gcgtgtatct attttaacct tatcacctgc ttctatgaag
    61141 aaggggaccg tcaaacgtaa accagtttcc gtgtaggcaa tcttgccact tccagaagca
    61201 gtattacctt ttacattagg ttcacattct gtgatagtct gaataactgt aactggtaat
    61261 tctataccaa gaattttacc tgagggagta agtaacaggc ttacttccat attttccact
    61321 aataacttat cttgttcacc aactaaactc gcatcaactg gtatttgctc gtatgtttct
    61381 gtgtccatca ttatatagaa atcaccatct cggtaaagat attccatttt tctacgttcc
    61441 acacgaactt cattaaaagt atcactttcg cgccaggtat tttctataac ttttccggtc
    61501 cgaacatttt tcagtttact acgcataaaa gcgggacctt tacccggctt tacatgcaga
    61561 aattcaacaa cttcccagag gtcatctttg aattctataa tcattccatt gcggatatca
    61621 gccatcgtag ccataaatat tccttgtatt tattgtttct gaaatatcca aaaaattaat
    61681 ctccttgtat ttgacaagga ttttctttct aattgtaaat tataaaagca ttatttgttt
    61741 atcttcttac catatcatat ctaagctttt ttaaattcta tatttatccc tatgtccagc
    61801 ttattttttt cttccccaat cccttgctct aattgaaaag attataccta ataaatctta
    61861 agcttatgtc caatttcgga cataagttgg acataagctg gacataagct ggacataagc
    61921 tacccatcaa ggcgtaggaa aaaaaatcct gaatgaagag aaaataagga taaaaaatgc
    61981 atatttttca gtctaaatcg ggtaactttc ttaatttctg cttgacgaaa tattgattta
    62041 agtttaattt ataataaagt ttatataata aaggatgtaa aaatatgtct ataaccttaa
    62101 aaacagtcca ggacaagaaa gaccttaaaa gatttgttta tctacccgag aaattaaatg
    62161 ctcatagacc agaatgggtg cctccaattt attctgatga tatgcgagta cttgacccca
    62221 aaagaaatcc agctcaccgc tattgtgata gtgtcttgct tcttgcttat gatggtgaag
    62281 aattaatcgg aaggatagct ggaattatca atcatagata caatgattat gcaaacacga
    62341 agacagctcg ttttagtttc tttgaagctc cagaccgctt ggaagtaagc aaaactatgc
    62401 tggattatgt agaaaattgg gcaagagata aaggaatgca aaagattgtt ggtcctcaag
    62461 gttttacgga agaagattca gaaggcttta tttatgaagg ttataatgag actcccactc
    62521 tgggatgcat ccaaaatcta ccttatgtta atgagcatat ggaaaagctt ggttacggaa
    62581 aagaagtaga ctggttcgtc tataaagtag aagtaaagaa agcaatgaca gagacttatg
    62641 ccaggctttt tgagagggca agccgaaatc ccaattttca cctgttggaa tttaccaaaa
    62701 agaaacaact taaaccctat gttcgtccta tttttgattt gatgaatgag gcttttatgg
    62761 ttctttatgg ttacacacct ctggatgaag cagaaattca atctatggca gaccgttata
    62821 tgcctcttct ggatcctcgc tttataaaag cggtggaaac aacagataaa caaatcatag
    62881 gttttatcgt ttctataccc aatatgagcg aaggtatcaa aaaggcaaaa ggacgcatta
    62941 ttcctttcgg tattttctat atgatgcaag cggcaaagaa aagtaaacaa ctggataatt
    63001 acctgggtgc agttaaagaa gaatatcggg gtaagggagt agatgttttg cttggctatt
    63061 cgcaattaaa atcagcgtat gaagctggct ttgaaattat tgactctcat catgtgctgg
    63121 aagataatat caaaatgcgt gctgaaaacg aaagagttgg cggagttatt tataaaaaat
    63181 atagacttta ttcaaagcaa ctataacttg atttgacatt atcaggtgaa atttgttcta
    63241 taatttaggc actcttcaag agcgtaatac tttattagtt atagttttaa aaagatgaat
    63301 ttgtaaacat ataatgagtg aaaaaataga ttaaatgaag agtaagcttc agcagtataa
    63361 gattaataga gagaaatgat ttgatattta gaaaatgaac caatcgtcca ttgacaaaat
    63421 agtcttaaag aaaagactgg tatcaaaagt aagcaggaag ttcactattt aatgagaaaa
    63481 tatatagttt ccatcgtggg gcgtccaaat gtaggtaagt ccacactatt taatagtcta
    63541 tgtcgtaaga gatcggctat tgtagatgca gaagtgggtg taactcgtga ccgtaaatat
    63601 gaagatgttg aatggaatgg taaagtcttc aaattggttg atactggagg tatcgtcttt
    63661 gatgataaag aagagttgac caaaaaaatt cggcatcaag cacttcttgc tatagacgaa
    63721 tccgatttaa ttctatttct ggtagatgca caaactggaa ccacagatat agataaagcc
    63781 attgccaaat tgttatatcc ccatagagat aaggttatgc tggttgctaa taaggtggat
    63841 aatgaaaaat atgaatggga actctatgat tttctacagt taggtctggg tgaacccttt
    63901 cccgttgcag caattaaagg tagaaatata ggaaactttc tggatgaggt tttaaaacat
    63961 attcctgaaa ctcaatcctt aagcgaaact caagaatctg ggccaaccag aattgccgtt
    64021 gtaggaaaac ccaatgtagg gaaatcctct attgtaaatc ttcttttagg taaagaaagg
    64081 cagattgtaa cagatattcc aggaactacc agagattcgg tagatagcta ttttcgctat
    64141 tatggcaaag aatatgttct ggtagataca gctggtctaa gacgcaaaac taaagttgat
    64201 tatggagtgg aatattttag tgttatgcga gctattgaag ctgttgaccg ttctgatgta
    64261 gtggtccttg ttcttgcggc tgatgaagaa atctcagaac aagaagtaaa aattgcttcc
    64321 tatgctcgca ggaaaatgaa agaaattata gttgtattta ataaatggga tttagtagaa
    64381 aaggataaca aaacgacagg taaatatgtt gcagagctct atcggaaaat gccatttttg
    64441 caattcgctc cagtgctttt tatttctgca aaaactggtc aaagaataaa tcggataatg
    64501 gaaacagttg taaagataga acaagaaagt gaaaaacgaa ttccgaccag tgaattaaat
    64561 cgttttatag aaaccgtagt agaaaagtat cctcccacgc atcgttccgg gaaagaagtt
    64621 aaaatttatt atgtgacaca agcaggagta aaaccaccca ctttcgtgtt tttttgcaaa
    64681 agacctgctc tggtgacaga aaactatcgt cgctatctca acaatcaaat ccgagaaatg
    64741 ttctcttttg aaggagtgag cattaagctt atcttcaaag gaaaaactga agatgaaagg
    64801 atggcagaat gaatttgacc ttactctgga taatcctttg tatttgtgcc tatcttatcg
    64861 gcagtatacc atttggctat ataatcggga tgattttcca tcatcaggat atacgtaaag
    64921 gtggaagtgg aaatatcggt gccactaatg ccttaagact ttacggagct ctttctggca
    64981 ttgcagtttt gattttagat tttctaaaag gttttatagt tgcctggttg ctatataaag
    65041 ttgttccaga taaatttggt tatttgattg gtgataaacc attaaatgcc tttattttta
    65101 atcttccagc tttgatggtc attttagggc atatgtattc tgtatttctt ggctttaaag
    65161 gtggaaaagg agtttccact gctctgggtg tgtttatcta catagctact atcccaatgt
    65221 tattatcttt aatagtattt ttaatcgtcg tggcaattac tcgctatgtt tctttaggct
    65281 ctatagtgac agcattttcc ttttttatca tagattttct ctggaatata ttggtaatta
    65341 aagattacac ttttccctgg tttcagttaa cggtggtatt attgataata tataagcatt
    65401 atccgaatat tcttcggtta ctggaacacc aggaaactaa aataagcttt aaaaaaggtc
    65461 aataataaat gtgtggaatt atcggaatct atggcaatcc agaagcagct cgtatggcag
    65521 ctctgggaat gtttgccgaa caacatagag gacaggaaag ttgtggttta gccgttagtg
    65581 atggtaaagt tattcactta tataaaagta tgggactggt aaaagaagta tttaccgatg
    65641 aagtacttaa aactctacca ggttatattg ctgtcggtca tgttcgttat ccaactaagg
    65701 gtgcagctac caaattcaat actcaacctc atctggtaga aacgctattt ggtcccagct
    65761 atgctcttgc cagtaatgga gatttggtta attatgacca gatacgagcc cgattagaag
    65821 ctgaaaatgt atattttaac agcgataatg acggtgaact attagtaaaa tatcttgcct
    65881 ggcagattat agctaaaaat aaagatgtag ttgaagccat ccgttgtctg atgaaagata
    65941 tcaaaggtgc ttattcctgt gtgttttgta ccaggaaaga actttatatg tttcgtgacc
    66001 cactttctat ccgacctatg atatggggta gaaatatcaa tggagatgta gtggtagctt
    66061 ctgaaagttg tgttctggat attttggatg tcaatggaag aagtgaagtt cccgctgctg
    66121 gaattattga agttaatgca gatggagtaa aagttataga aaataatcct gcccaattca
    66181 gacagcaaaa tcataatagc tattgtgtgt ttgagcatat ctacttttct cgtccagata
    66241 gctatcattt tggagaaaat gtctttgaag ttagagaaga tatagggcgc aaattagctg
    66301 aacaggataa aggtctggaa gctgattgtg ttgttccagt tcctgattcg gcaaatttta
    66361 tggcagtagg ttattcagca gccagtggaa ttccgctatc tttagggctt atccgtaatc
    66421 actatattgg tagaactttt atcaaaccag aacaaactat ccgggatgag agtgttcgtc
    66481 agaaatttaa tgtattgccc aatttcttta aagggaaaaa agttgtgctc gtggatgatt
    66541 ccattgtgcg tggaacgacg attagaaaga tagtgagaat gataaaatta gcaggagcag
    66601 aagagattca tcttagaata ggtagccctg ctatctgtta ttcctgctat tatggtattg
    66661 atactcctca tagagaagaa cttgcggcta atcgttattc gctggaagaa atgaaagaaa
    66721 gaattggggt taatagctta aaatatcttg atatgaactc tttgcgagaa tgtttaaaag
    66781 agccagatga atattgttat gcttgttttg atggaaaata tcctctggag aaagaatgat
    66841 agctgaaatt attaaaaact ttcaagggaa aagagttctc gttttaggtg atgtaatgtt
    66901 ggaccattat gtctggggtg aagtggaacg tatctctcct gaagctcctg ttcctgtcct
    66961 ggaagtgcaa aaagaagaat ttcgtttagg tggagctgcc aatgtggctc ttaatatcaa
    67021 aactcttggt ggagaaccaa tacttttagg tgtcattgga aaagatactg cagcaaatga
    67081 ccttatccga cttttaactg aaaaagatat agatactgat aaattggtta tagattcctc
    67141 tcgttctact actttgaaaa ctcgtattgg agcaatcaat cagcaaattg tccgtattga
    67201 ctatgagacc aaaacagata ttaatcttga gacaatgcga aagcttttaa ccaatctgga
    67261 aggtctgata agcaactgtg atgctatgat tatagaagat tataataagg gtttgctaac
    67321 cgagaaagta attgaagaag ctctcagata tgccaaagaa aagaatatcc ctgtagcagt
    67381 tgaccctaaa cagaagaatt ttatggctta taaaggtgtg gatatcttta aacccaacta
    67441 taaagagtta caaactataa gcggaaagat atttgaaaat gaagaagaat tcattcaaac
    67501 tgcccgtgat tttaagctga gaatgaatat taaaaactta attgtcacgc gaggtgctct
    67561 gggaatgtat gtttttgaag gtagaggtgc acatatccat cttcctactt gtgcccggga
    67621 agtatatgat gtctcaggtg caggcgacac cgttattagt gtcctgacct tagcttatat
    67681 tagcggtgct gatatatatt cagcagctga tattgccaat catgcagctg gagttgtttg
    67741 tggtattaag ggaactgcca gcatcaccaa agaacaatta ctatcaagtt acaatgatta
    67801 aagataaaat aaaaagctgg gaagaagcag ctcttctatg tgacaatttt cgttcgcaag
    67861 ataaaaaagt agtctttact aatggatgtt ttgatatctt gcattctggt catatccagt
    67921 atctggaaca ggcaaaagct ctgggtgata tcttagttct cggtttaaat agtgatgata
    67981 gtgttcgtag aattaaagga gcaccacgtc ctatcgtatc agaaaaggat agagcttttg
    68041 tggtggctgc actgcaatgt gttgacttgg tagttatttt tgagcaggat acaccttatg
    68101 aacttattcg tttgcttaaa ccagatattc tggttaaagg tggtgactgg aaaactgaag
    68161 atatagtggg tgcagatatt gtgcaagcat atgggggaaa agttctcagt ttaccctatc
    68221 gttcaggtat atcaactact gaattattcc gtaagattgc agaggctttt gctgatttta
    68281 caatgaagga taatggaaac tatgaataat gaaaaaggtc aatatccaca ggaagatgtg
    68341 ggaactgtta ttgaagtaga aggggaaaaa gtgcgagtgg aagttgtgcg aagtggaggt
    68401 tgtaaaactt gttcaatgca aagcttttgt ttcggacgca acactcctgc agtttttgat
    68461 gtggaaagtg aacttgagtt aaaaccagga gatagagttg aactggaaat agctcctgct
    68521 tcaagaatta tatcctcact actggtcttc ggtttaccca ttctttgtct ctttctcgga
    68581 tatttgattg gtagtatctg gttggatgaa cttgcctcta taggtattgc ttttgctgcc
    68641 acagctttaa gttatattat cttaaaattg atagaccgta aatttgggaa aaaggtgcaa
    68701 gtccgtattg gaagaaaatt atgattatta gattgaatga tatggtattt tatggatatc
    68761 atggtgtgaa tgatgaggaa agaaagctgg gacagcgttt tattgttaat ctggaaatgc
    68821 aaactcatcc tgaaacagat tctgtgatta agcgattgga agatacggtt gattacacta
    68881 aagtgtatgc agaaatccgt gaaattatgg aatctcgtca attccttctc ctggaaaatt
    68941 gtgctaatat cgtagcagat aaacttctgg ctgattttcc tctgatctgg aaattgaaat
    69001 tatgcattca aaaaccgtcc gttcctattc agggaatttt aagaagtgtg gaagttgaag
    69061 tgcaaagaag tcgtgaatga ttgcttatct ttgtctgggt tccaatctaa ataatcctga
    69121 aactcaggtt cagcaagctc ttcaaaagct tagagaggac cctaatatcc gtattttgaa
    69181 aagctctact ttaatccaga caaagccagt gggcaattta aatcaacctg atttttacaa
    69241 tcaggttata gaggtggaaa catcttattc ttcggatgag cttttacaaa aagctttaaa
    69301 tctggaaaat caaatgggac gtgttagatc tgaacattgg ggtccaagaa taatagatat
    69361 tgacatttta ctttatgaag atgagataaa acatacccag gatttgattc tacctcatcc
    69421 agagatgact aagagaaaat ttgtgttgga acttttgtgt tcaattgcac cagatttgat
    69481 tcatccagtt ttacataaaa gtatagccaa ccttttgaaa gacatagaaa aaaaggaaga
    69541 ttaagcaatg tctaacttat cagctataat tcttgctgcg ggtaaaggca caagaatgaa
    69601 atctgaacga gctaaagtca cttttagctt tgctgaaaaa cctataattc agagggtagt
    69661 taataccgcc ttaaaagcta attgttccaa aatctttgtg gtagtaggtt atcaaaaaga
    69721 cagcgttatt gcttgtctgg aagaagaccc tcgtttaatc tttgtggaac aacctgaaca
    69781 gttaggtact ggtcatgctg ttatgatggt tgaaccagtt tttcaagaaa gagaagatga
    69841 tgtttttatt atctgtggtg atgtgcctct tttaaaacca gagacattac agaaactgta
    69901 taatgagcat tcttcctcta aagcttcttg tactattcta acctctatta tggatgatcc
    69961 aggaagttat ggacgtattt taagaaatgc ttctggtgag ataactggca ttgtagagtt
    70021 taaagatgca actgaagaac agagagctat taaagaatgg aatactggaa tttactgttt
    70081 ccaagctggg gatatgtttg atgcactcaa acaaacttcc agtgtcaata atcagaacga
    70141 gtattatctc actgatgtag tttctgtcct tgccaagcaa ggtaagaaaa tatcctccgt
    70201 tattttagat aatcccatag aagttgctgg tgttaactct caagaacaat tagctgaatt
    70261 ggaagattta tttatagcgg aaattcgcaa acattggttg aataatggtg tggtaattca
    70321 taatccacga actgtttata ttggcgaaga ggttgatata gaaacagatg tagagattgg
    70381 gcaaaactca attatacgag ggaagagtga actaaggaaa ggagcttata taggaccaaa
    70441 ctgttacatt gaagataccg ttctctgtga tagcagtttc ctggaaggat ataatatcct
    70501 gattagtgct cacattcctg aagggcatat tcttgaattt ggcgaaagaa caattgaaga
    70561 tattgactat gaataaagaa tttttatact ctccctggcg tttaaattat attgaaggag
    70621 aaaaaactga tgactgtatc ctgtgtcgtt atcaggaaat ggataaagac gaggaaaatc
    70681 taattgtcta tcgcggaaaa cattgctatg taatgttaaa tcgctatccc tataacaacg
    70741 gtcatataat gttagtgcca tatttacatc ttaaatctct tcatgagttg cctaaagagg
    70801 ttattgagga tttaggctct ctttgtcagc tcagcgaaaa agtgttgttt gctgcttata
    70861 attgtgaagg tatcaatgta ggtataaatt taggaagagc tgctggagca ggaatagatg
    70921 aacatctcca tattcatatg gttcctcgct ggattggaga tagcaatttt atgagtgtta
    70981 ttggagggca gagagtgatt cccgaagctt ttgaaaaaag ctggcaaaga ttgcatcaag
    71041 aatttatata tcagcttcaa ttgaagaagt agggcattag gtttgacagc aaagcgctcc
    71101 tcaaataaat ggtttccaag taaaagcttg ctctgggttc ttattgtagt cttgacagga
    71161 gttctgatta ccttaatata cataagattc atctctccta agagtgattt tcaggacctg
    71221 gatgagaaga atttacctgc tattaaagtg attatcaaaa atggatgcgg ggtagaaaac
    71281 ctggcaacag actatgctaa ttatattcgt aacaagaata tagaagtaat cagggttgct
    71341 gatacacctc atccaattta taataaatca ttgattgaaa tgaaaaaaga agactggcaa
    71401 gatttgaaaa ggttacaaaa aatgacaggt atccaacgct atactttagc ggttgattca
    71461 gatagtgaag ctccttttat tatcatatta ggagctgatt atgatacctt tatgaaacgc
    71521 taaaaaggag aagattttgg cggataatat aaagttaaca gatgtcttaa actggcttaa
    71581 cgaaaagcaa gcagaacata ttgcagtata tgatgtagca aataactgta gttacacaga
    71641 ttttattgtg gtatgtaatg gttcagctga tttacacaat aaagctattg ccaattatat
    71701 cttactgatg gcaaaaaaga gcaaatatca aattttaggt aaagccggtt tagaatatgg
    71761 tcaatggata ttggtggatt ttggagatgt aatagtgcac attttccgcc aggaaaaaag
    71821 agaatattat gatattgacc agctgttttc agaacttaca gaaccaatta aggaagagaa
    71881 agatttatga taaagaaaca aattgctgat aatgtgctgg aagcattaga taatctggga
    71941 ttaaaacaca ccaaagaatt ttctgtggaa gtacctaaca atccagattt tggtgattat
    72001 tcaactaatg ctgctttaat gttagcaaaa gagaatcaaa ttgctcccaa acagatggca
    72061 gaaaagctgg ctaaagagct acgcaagaat aagcaattca aaagtgtaga aattgctgga
    72121 ccaggtttta ttaatttccg catagctcct tctctttatc aaaaagtgct atgggatatc
    72181 cacagacaag gaaagaattt tggctcttca gattatggta agggcgaaaa agtccttatt
    72241 gaatttgtta gtgccaatcc tacaggtcct ttaaatattg ttagtgctcg ggcagcagct
    72301 tttggtgata ctctttatcg tcttatgaaa aaagtaggtt atgaagctgc tcgggaatat
    72361 tatgtaaatg atgcaggaaa ccaggtggat attctggctg aaagtttaga acttcgttta
    72421 agagagattc agggtgaaaa cattggagaa tttccttacg aagcttatca tggagaatat
    72481 gttaagcact tggctcataa gcttaatgct gctgaaggtg tgcggatttt tgtgatgcct
    72541 gaaaaagagc gaactgaaca tcttaaagaa tttgctctcc gcgaaatttt ggaaatgcag
    72601 cgtcaaagtc tggaaaaatt tgatgtccat tttgataatt gggtctcgga aagaacctta
    72661 cgccaggaag gagtggtaga agaggtgcta agctatttga ctgaagctaa ctgcacttat
    72721 gaaaaagatg atgctatatg gttctcttca actaaatttg gtgatgataa agaccgcgtg
    72781 ttaatgaaaa gtgatggctc tatcacctat tttgttcctg accttgctta tcatttaacc
    72841 aaaattcaaa gaggatatac taaactaatt gatatttttg gacctgacca ttatggctat
    72901 gtaccacgtc ttaaagcagc atttagagct ctaaattatg atgagaatat gctggaaatc
    72961 atctttcttc agcaggttaa tctgtatgaa aatggggaac gggtaaaaat gagtaagcga
    73021 gctgggaaaa tagtgacaat ggatgattta atctctcttg taggtaaaga tgcagctcgt
    73081 tatttcttta ttgctcgtaa agccagtgct catctaaact ttgatttaga cttagctttg
    73141 caaaaaaata atgaaaatcc agtctattat tgtcaatatg cacatgcacg tatttgttcc
    73201 attctcagaa aagctcgtca ggaaaacata tatccgaact actttaaaaa tgaactaatt
    73261 cggaaacttg tccgtcctga agaattagac cttatcaaga aaatggctga tttccctgaa
    73321 cttttgataa acattgtaga taaaagagaa cctcatctac tggctaatta cactcatgaa
    73381 ctttgtgctc tctttcatca tttttacaat cattatcggg tcataaatcc caaaagaata
    73441 gagctttctc aagcaagact tttgctaatt gagacagtga aacatactct tgagatttgt
    73501 ctggacttaa tgggcatcag tgctcctgaa aaaatgtaaa tagaaaatgc gagtaattcg
    73561 tcttatgcaa gattcggacc ttgatgaagt tatccgcatt gaaaaccagg tttttcatcc
    73621 tccttggccc aaagaagctt ttatggaagc tccttgcact tattcctggg taatctgtga
    73681 agatgaagtc ttgcaaggat atatagttta tcacacggtt ctggatgaag gagttatcct
    73741 taattttgct attgatccga aattccagta tcagggactg ggcacttatt tattgaaaga
    73801 aactttgaat tatttaatag ataacggaat ccgaatgttt ttcctggatg ttcggacttc
    73861 caatatggtt gctcaagact tatataagaa atttgatttc catcccatcg ggaaaagaaa
    73921 aaattactat agtgaaccga ttgaggatgc tatcgtaatg gtgaaataca tagatgaacg
    73981 gaacaacgaa aatatatgat tatctggtaa ttggtagcgg cattgctggt ctaacatatg
    74041 ctcttcaagt ttcacgctgg ggtaaagttg ccattattac caagaagagt ttatttgatt
    74101 gtaataccga ctatgctcaa ggtgggatag ctgctgttct ggaccctcag gattcctttg
    74161 aaaaacatat tgaagatact tataaagcag gagctgaact cgggaaaaag aaagttattc
    74221 aacagattat tatggaaggt ccaaaactta ttcaatattt gattgacctg gggacagatt
    74281 ttacgcgtaa agatgagacc tatgataatt gtttggagaa cctttctctt acaatggagg
    74341 gtggtcacac ttttcgccgt attgcttatg cagaagattc tactggtcat caaataatgg
    74401 aaactttaac caagcgttgt cgcgaaaacc ctaatataga catttatgaa tatcatattg
    74461 ccatagacct tataacacag catcacattg ttcaggatga tggcttcatt ccaggaatta
    74521 cctgctgggg tgcttatatt atggatgatg ccaccaaaga gataagcatc ttcaaagctc
    74581 gtaaaactat gcttgcgact ggaggtgctg ctcgcattta cgctcataat accaatccag
    74641 atgttgcaac tggtgatggg atggctatgg caagattagc tggagcacgt ctggctaata
    74701 tggaatttgt ccaatttcat cccaccgcgt tttggtctcc tactggtgat acttttctca
    74761 ttagcgaagc tttgagagga gaaggtgcta ttctacgttt acaagacggg agcactttta
    74821 tggaaaagta tcatcctcaa ggtaatttag cacccaggga tgtagtttcc agagcaattg
    74881 atgcggaatt aaaaaagcgc ggagagaaat tctgctatct ggatgccaca agtattcctg
    74941 ctgaaatatt acataagcat tttccccata ttgatagccg attaaaaagt tatggtatag
    75001 attttactaa agaccctatt ccagtttttc ccgcagctca tttcttttgt ggaggagtgt
    75061 tagctactat tgatggtata accgatatta agaatttatt tgctgccgga gaagttgcct
    75121 gtacaggact tcatggtgcg aatcgtctgg catctaattc tctactggaa gctttagtta
    75181 ttggctggag agcaggaaat catccttcca atctggaaga agtgaccttc ccagaaattc
    75241 ttccctggaa acaaatggag gagttcaatg agcacgaatg ggtggtcata tcacataatc
    75301 gcgagattat tgggaccatt atggatggct atgtaggtat ccgtcgttca aggcgtttat
    75361 taaaatatgc cttatctcgt atggaaaata tatacaacga ggttaacaat ttctatcaac
    75421 ataatgcagt gcggagagaa gtggtggaaa ctcgtaatat ggcagttatt gccattgctg
    75481 ttatccgtag tgcattgatg cgtaaggaaa gccggggtgc acattttctt atagatgccc
    75541 ctgtgccaaa tgatgaactt tacaagatag acactataat ttaaatggaa tttatgaaag
    75601 gaatatttat cacttttgaa ggtattgaag gaagcggaaa gagcacgcag acaactttgc
    75661 tttgtgctgc cttagaaaat cttcattata aggtttttcg cactcacgaa cctggaggtc
    75721 ctcctattgc tgaggaaatt agagagctct tacttaatac taagaataag gaaatgctac
    75781 ctgaaaccga acttcttctt tattctgcaa gccgtgctca acatacgggt gaatggattt
    75841 tacccgcttt aaaagatggt aaaattgtcg tatgtgaccg ttattacgat tccacctttg
    75901 cctatcaagg tgcagcaagg tctttagata tggagtttat tcggattctt actgattttg
    75961 ctacctatca tactaatcca gatttaactt ttcttttgga cttatctgtg gaagaaggca
    76021 gaaaaagaat aaaaaaccgc tatccagacc gtttagaaag tgaaaaagac gagtttcatc
    76081 aatgtgtgcg taatcaatat ctggctctgg caagggaata tccagaaaga tttgtagttc
    76141 tggatggttc tttgcctaaa gagaccattc ataatatggt gttaaccaaa gttttagact
    76201 atataggagc tcaatgtgaa ttataaaaaa caacgccggg caattattac cattgttgtt
    76261 gtatggattc tttcagcaac aatgctattt actgctacca gtctttttgc tcagaataaa
    76321 tcatctggta gcgatctcta ttctcaattg ggtctgttta gtgaggttct gcaaaagcta
    76381 aagcaaaatt atgtaactga gcttaaagat gaggaattga ttaaggcagc cattaatggt
    76441 atgctcagtt ccactgatcc tcatactact tattttccta aagaagagtt tgaagaattt
    76501 accacttcta ctaaaggctc ttttggtgga ctgggtattc agatagataa aataggggat
    76561 tatataacag ttatttcacc gattgaggga actcctgctt ataggatggg tattactacg
    76621 ggtgatagaa ttattaaagt agatgatatc agtcttattg gtgcaagcac agatgaagcg
    76681 attaaacata tgcgaggtga agtagggaca acggttacta ttactatcag tcgtcctgga
    76741 gtggaaaaac cactggaatt tacacttact cgtgaagtca ttaaaataaa gagtattcct
    76801 tactatttta aactggataa tggagcagga tacatccgta tcagtcaatt taatgaaaat
    76861 actgtccaag aacttagaac tgctttgaac tctctggaaa atgaaggaat aagagctctg
    76921 attattgact tacgctggaa tccaggggga ttattagacc aggcagtaga tacggttaat
    76981 gaatttattg gacccaataa actggtagtg gaaactaaag gaagaattcc aacttctaat
    77041 cgtcaatatt tcacccgtta caacactaaa tctcgtaatt atcctattat tgtgctggta
    77101 aatgaagctt ccgcaagtgc ttccgaaatc tttgctgggt ctatgcaaga ttgggataaa
    77161 ggattagtta taggtaagcc tacttttgga aaaggaagcg tgcaacaatt gattcctttg
    77221 atgaatggcg gaggtcttaa agttactact gcctattatt atattaaatc tggtcgctgt
    77281 atccataaga atataaatga taagattctc ttaggtaaag aagtaaccga aaaagaccag
    77341 gaagccgaaa aggaaaagaa cctacagcag gtatattata ccgttaacaa gagaatcgtt
    77401 tatggtggtg gaggtatcac tcctgatatt gaagttgatg ctgatttaat gaccaatttt
    77461 ggtatggaat tgcgccgtca aaatgctttc ttcaattttg ccgtggatta tatggtggaa
    77521 catcaacata aactggataa aaactttgtt gtgacagacg agcttatgaa tcggtttctg
    77581 aattatgttg aagataaagg tatatcctac acgcaggctg atttggatag caccgatacc
    77641 tatatccgga ctatgcttaa atcagaactc attcgtaatg tctatggtga tacggaagca
    77701 tataaagcca gtatcaacct ggataaacaa ctccagaccg ctatcaatct tttaaataaa
    77761 tacccatcta tggatgctct ctttgattat gctgccaccc aaaataagag atagttgaac
    77821 ttaactcatt tatgaaatag caaaaaagaa gatattgtca tttcttcata agctactgga
    77881 attctgctaa atctgacaat atcttctttt tcttttagac ctaaaaaaag aaaaattatt
    77941 ctattccctt ttcttccttc aagtttacct ttttattgtt catccttaat aaaattccca
    78001 gacctatttt aactgcataa aatgcgagaa gagacatagc accatcaatt aataaatctt
    78061 ccatcgtatc taaaactccc agacgagtat cttttattcc atatattatc tcaagagaac
    78121 gagacctttg catatcagtc ttaaaaagag aatctattaa gaattccaca atttcccata
    78181 atgctcccgc ggttatggca aaacagagag tgaaccaagc gataaaagaa ggggttaaaa
    78241 ctggttctcc tatatcataa ccgctctgag aatatattaa tagaaagcct atgcctgcag
    78301 tagtgagcga tgaaccacaa tgtaatataa attcaaaatg agggatagat gctattggtg
    78361 gatagacaat ggggaaataa atcattatta acagaattat ttgtacgaat aggggaatac
    78421 ggagttgatt ttgctttccc acagcaatgg gaacaaagaa aaataaaagg gttatggcag
    78481 aaaatcctgc taattcccaa gctacactat gaagatgata acctgtaccc agagctgcta
    78541 atagaattgc tatatgttgc cattgctttg ttctattatt catatttgtc tcatccttag
    78601 ccatctctat catccatcaa ttttttttaa ttttcttttt aaagaataat caaatttttg
    78661 tcaagtcaaa ctcctgctgt ctataacttt cagccttact ttttctttat aataccacta
    78721 attatcttcc ataattattt tttatcgtca gtatttttaa tatctttgat catgcctcca
    78781 tccttccttt atcttagcca tttcttgaga aaagtaatag gaaagagcca agggagagct
    78841 aagagagaac tgtagtaggt tgtcctggga aagattacct aaatcaggta taatggtgag
    78901 atgaaatagc aaatagtaag atataaaaac ttcattaacg agtatgaatt accatagatt
    78961 taggactgag ttaaacatat tatatttagg ataaaaatat tataggatag ataacaataa
    79021 tataaacttg caaatatcaa tttcatcata ggatgataac ctaattcgct ttataaaaaa
    79081 agtgctgcta ttcaacttat taaaatagca gcacttaaat ctaagagttt atatttagta
    79141 gtagttaata cattacaaca taaccttgtt tattctaccc taacgggtaa tctatctaaa
    79201 cattgctaac tacactgaat aatatatagt tacgcattct ctgacagtac tcccagcctt
    79261 taaaaaataa aagataggtg aattaccaaa ggtaatttca accttatttt ttaggaggaa
    79321 gtgtagaagc attaacttta gaatgtttgt cggtagcttt aatactccac ccttttaccg
    79381 tgtcaaaatg caggactgta acatcccata aatcgccact atcggataag tagccataat
    79441 aatttactgg attttgctct cctttcctga ttactaccac actgtttgtg ccaatggcat
    79501 tttcactgtc atgatcactg taatagtgca cttgcaccaa ataatctcct gggataacag
    79561 atggagtagt aatattttca ggaccgtaac catttacatc atcaacatca agtgataagc
    79621 cacttgcagt ttgacgatgt gcccaataac atctttcacc attagggtca gtaacccaga
    79681 ggtctacatc cgttccataa gtatcccaag ttagagtgca atgtaaagcc attaaaggaa
    79741 cctgtgaaga gaaagacatt gtattttcag ctaccagagc tccagaaaca gctataacat
    79801 atactgtatg ttgaacttcc tcgttggacc cggttaatac tatttcttga gagaaattgc
    79861 cattattcaa attgaggtca aaagtatgct cgctgtcatt atccagaatg agtaatgcag
    79921 tggttaattg aggattggct atttttccgt ttatattaac aatcctgtta gttaccacat
    79981 tagagatttc attaatggtt acatagaaat ctataggaag ctctatagta tcagtaataa
    80041 gtacattatc attgttttga ttttgtgttg taaattgata ttgtagagta agttgatttt
    80101 ggcttagttc tttattgata gtaataggaa cacttaccgg taaggtttgt ccagcctcaa
    80161 tagttacatt agagggatta aaagaggaag aaaaaccaac ggaagttact ataccttgat
    80221 agccagtcaa acttgttgtt cctgtattag taacgaagag ttttatagtg gctctttccc
    80281 ctggattgag tttattatca ataaagtttt catcttcaat ttcccaatta tcaataacca
    80341 gtttaccacc agaagaagcg acagtaatac ttctggattt atgattaccc atattatcgg
    80401 aaatttctac ggcaatagta tattcctttt cttctgtggg agtgaaggtt agatctgtcc
    80461 ctttagcttt aaaacctgta taagtaccac ctgaaaccgt ccattgaaaa ttgagagcct
    80521 gtccaattgt gctaacgcaa gtggtgtgtg catgtacggg ttgattaacg gttatgcaac
    80581 cttcaacgac agttaaatct tcaattatga ttgcattctc agcaagtttc agaagatttg
    80641 tggcaacttg tgtcaaacca gcttgagcga tttgcaagtt atcatagaca ccacgaatga
    80701 aatcttgagc cagagcaata acctgatact cactttcagg cagctgtagt tgtagatttt
    80761 gaggtgcatt cataatgata gtaatacgag gattatcttc aggcatatca gaccttgaaa
    80821 taattaaagt tttccctgtt tcaatgggag gatttccggt ttcggaagaa ttgatgtcta
    80881 ctgtaagtgt tccacaatct tgaggagttt caccttcttc catcatctga tccagaattt
    80941 cattattcca ttcaccaaca tttccaccat aagcatctcc cacagcttta actccagttt
    81001 ctccaagttc ctttgctgtc tcagcagttg ccgccctttc atcatcgctc catagcataa
    81061 ccccagagcg agtttgcatt ttgattgggt cttcatcact taaattaaga taggcattgg
    81121 ctttttcctg aggtgttgaa cctggtaaat catcataagg aatagtgtaa ttgcaatctt
    81181 caggggaagt ggtaggattc cagtttaaaa tcatttcacg gattttagcg tcacgagcat
    81241 tattacgttt ttgaatggtt tctgcccatc cagaaagtaa tggtatacca gattcgggat
    81301 catttaaaac ctgacggata ctttgtttac cacttaaaac tctccagcca gaacgaacca
    81361 ttctcccacc agagacgacc gtatcctttg ctttattata gatacctttt gccacattgg
    81421 ataacaatcc tttactttct ccataaggta cgatggaatc ctctgcccga gctaaaacag
    81481 caaaatgatc ggaaataaca ttacactgat tgtaatattc atctaccata ccgtcaattt
    81541 gactgaagtc ataccgattt tttgacttat tccctaaatt taaaatggca ttttgtattc
    81601 caaccattgt gccatcatgc gattcaagag tggaagttaa atctgattga tactgccagt
    81661 aattatttag agaagttaca atgtcagtgt taatcggttg atgatccgca tcatctttct
    81721 gacaagcagc aaagataaat aaaaacatac aaaataagat gacaaagaaa tgttttgttt
    81781 tcatcgtcct caactcctta atatgtttaa ataattttgt gattaaatag ataccgtgag
    81841 aattgctata ctattatttt ttacaatcca taaatgaatg tagagtaaga tatagacaaa
    81901 atcttagcag tactattatt cacattattg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    81961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    82021 nnnnnnnnnn naatgtagag taagatatag acaaaatctt agcagtacta ttattcacat
    82081 tattgcccat ataaaacttt attaatatat gtcaatttaa aaaattaggg gaataatctt
    82141 gaagactttc ttcatctact gatttcttta gaattccttt tattttcaat aaccgcagtt
    82201 ctaaggataa tgtgattcag gtagctttaa ttaaatgttt tactcaaaaa ctgagagcaa
    82261 aagttagcaa gctcattacc agatttaatt tctttgacag gttttagggt ataaaataag
    82321 aaggctttaa taaaagaaat ctggagttga aaggatgaat aaccaaattt tggattttct
    82381 ggactttctt aatggttcac ccacttcaat acaagcgtct cacgaaatag ttaaaaggct
    82441 ggaggaaagt ggttttaaag ctttaaaaga agaagaacaa tgggcacttc aagttggtgg
    82501 taaatattat gtttgtcgtg ataccagtgt tgtagctttc actttaggaa gcaaaaatct
    82561 tctggatact ggatataaaa ttggagcagc ccacatagat agtcccagtt taaagataaa
    82621 accagaaagt ctgaagacag aaaatggtat tacccgtgtt tccgtagaag tctacggagg
    82681 tcctataatc ggttcctgga ttgatcgtag tttaagtatt gcggggaaaa ttgcaattaa
    82741 gcaagaagat aatcttagat ttttaccagt aaatttgaaa caacccgtag cagttatacc
    82801 caatgctgca cttcatctta atagagagat aaataagggt tttgaatata ataagcagat
    82861 tcatctgcaa gcgatattac agagtaaaga aagtgaaaaa aaccccttga tggaagcttt
    82921 agctaaagct gcaggagtgt ctgtggaaaa tatttctggc agtgaactct ttctttatga
    82981 ttttgagcct gcagagattt gtggtttaga ccagagctta ataatgagtg ccagattgga
    83041 taatttagga atgactcatt caataatgag tgcaattcgt gaagtagata attctgagag
    83101 cacctgtgta gccgctttat ttgacaatga agaaatcggt tcagaaacca gacaaggagc
    83161 ggattcttct tttctggaaa atgtattgga aagaatagcc ttaaacatag gattttctcg
    83221 ggaagaatat ttgaggatat taagtaagtc atttcttatc tctggcgata tggcacattc
    83281 ctatcatcca tcttatcctg agaagtacga tccaagttat gctcctgtaa tgaatggagg
    83341 accggtaata aaatggaatg cgaattataa ttatgctact aatctggaaa gcagtcaaag
    83401 atttaaaagg ctctgcgaaa tagctgaagt tccctgtcag acctatatta tacgcagtga
    83461 tttactatgt ggtagcaccg taggacatat tatatcttct cgtttgggca ttcctgtggt
    83521 tgatgtaggt aatcctctct gggctatgca ttcagttcgt gaaacagtag gtgtggaaga
    83581 ccacataaat atgataaaag ttttaaaagt atattatcaa taaaatatat aggagaatca
    83641 taatggcgac cttcaaacct ttcaaagcag ttcgtccctt gcctgaatac gcatctgaaa
    83701 tagcttcttt gccgtatgat gtaatggatt cggatgaggc aagaattgaa gcacaaaaac
    83761 atcccttgag tttcattcgg gtggaaaaac cagaagtaga tttaccacca gaaattgacc
    83821 tttatgatcc agcagtttat gctaaagctc gcgagaatct ctatcgttat ctgcaagaag
    83881 gctatatgaa acaggatgaa aagcctatgt attatgttta tagagagatt atgaatggga
    83941 gagaacaaaa tggcttggta ggattggttt cagtggatga atatatgtct ggtgtgataa
    84001 aaagacatga gctgaccaga gaagataaag aacaagaccg tattcgtcat atagatgcct
    84061 gtaatgccca ttcatcccca gtgtttctaa cttataagca tcaagattct atcaatgaag
    84121 tagtagaaag agtgaaaaaa aaccatgccc ccgaatatga cttcgtagct gatgatggta
    84181 taagtcactc tctatggtta attgataatc cagaagatat tgaagtcatt caaaaaagtt
    84241 ttgccgcact ggattgtctg tatgtagcag atggacatca tcgcactgct tcagctgcta
    84301 aagttggtct attacgtcgt cagcaacatc cagattatac aggtgaagaa gagtttaatt
    84361 tctttatggc tgttattgtt ccagataatc agctcaagat ttacgattat aacaggatag
    84421 tgcgtgattt aaataatcat acggtagaaa cctttctgca tttagtagaa gacaagtttg
    84481 aagtgacaaa attaccagct gtgagagttt ttagaccaac tcataagcat gagataggta
    84541 tgtatcttgc ccattgctgg tactgtctta gacctcgtta tggcacctgg gatgccagca
    84601 atcctgtagc ttctctggat gtttccatct tgcagaataa tcttttgcgt ccgattttag
    84661 gaatttctga ccccagaaga gataaaagaa tagattttgt aggtggtata agaggattgg
    84721 aggaattggt tagaagagtc aatagtggaa gagaagcagt agcttttgct atgtatccca
    84781 catcagtaga ggaactaatg agaatagctg atgcgggaga aattatgccc cccaaatcaa
    84841 cctggtttga acccaaactg cgttcaggcc tttttatcca tttactggaa tgaaatatta
    84901 ttatatagta agtttaggct gttccaagaa tctggtagat agtgagatat ttgctaatat
    84961 tctggaaaag gcaggttatc aaccctggga tgaaagtgaa cctatagacc tggttttagt
    85021 caatacctgt tccttcattg aatcagcttt aaaagaagga gaacaagttc tttcagaatt
    85081 ggtagaaatg aaaagagaag gtaagattaa aaaacttgta gttacaggtt gtttgatgaa
    85141 tagaggttta ggggaatttg aagaagcttt tcctgaggtt gatagctgga ttggactgaa
    85201 agattttgct gctatggaaa cattgctggg gcttacatct caggcaaaat atcaaagaac
    85261 ttcaattgat gaagtttatc atcgctattt acttataagt gatggctgtt ccaatcactg
    85321 cacttattgc actattccct ctattcgggg tgagatgaaa agcattccta tagaagtatt
    85381 ggtggaagaa gcaaaagaaa tagcagatga agacaataaa cactattccg agctaattgt
    85441 tattgctcag gatacagcaa attatggtgt ggacctctat ggttataaag ctttgccaga
    85501 attgctggaa gagctaataa aacttaataa atataagtgg attagagtgc tatatatgca
    85561 tcctgaccat tttgagactt cctggttgga gctctgggaa aaacatccag agctcttgcc
    85621 ctattttgag attcctattc agcatatttc tgatccaatc ttaaaagcta tgggaagaaa
    85681 aaaagggtct actgaattaa aagaaatgtt taataagata aaagataagc ttcctcaatc
    85741 tgttcttaga actaccttaa tgataggatt tccaggtgaa tcaagacagg attttgaatt
    85801 gctaaagaat tttgttaatt caactccttt tctttatctg ggaatctttc cttattccag
    85861 agaagaaggt actcctgcag ctcaaatgga ccatcaaatt ttccccagaa ccatacggac
    85921 tcgttataat caattagtaa agctgcagca acaatctctg gaagaaatta tgggaagcta
    85981 tattggtgag atcttggaag ttttaataga agattatgca gatgatattg aagaagctaa
    86041 ttatattgga agaacctggt ttcaagcacc tgatatagat ggattgacat ttgttaaagg
    86101 agaaaatctg gaattaggta aaatctatcc tataaaattg atagatataa taggaacgga
    86161 tttatttgga gaagtgatta aggaaggagt aaaatgaaag ttgcactaac tggaataaaa
    86221 ccaacaggtg tgccacatat aggaaattat gtgggtgcta ttcgtccagc tttagaactg
    86281 gctaagactt gtgaagccag atattttata gctgattacc atgctttgaa tgcctgtaaa
    86341 gacccagagg aaatcagaaa aatgaccctg gaaattgccg ctgcctggtt agccagtggc
    86401 ttaaacccgg agaaggtcct tttttataaa caatcgctgg tcccacaaac ttttgagtta
    86461 atggttattt taatggcatt cactcctaag ggtttgatga atcgtgctca tgcttataaa
    86521 gcaatggtac aagccaatct tgaaagtgga aaggattctg atgatggtgt caatatggga
    86581 ctatatactt atcccgtatt gatggcagca gatatacttt tatttgatac ggattatgta
    86641 cctgttggca gtgatcagtt tcagcatata gaaatggctc aggatattgc tcaaagtttc
    86701 aattataatt atggtaagga atatttaaag atacctcaag caattgccag agaggaaact
    86761 aagacccttt taggattgga tggacgcaag atgtctaaaa gttatggaaa tactattcca
    86821 atgtttgctt cggaagaaga actttataag ctggtgatga gaattaagac aaactctcaa
    86881 acagtagaag aacctaaaga cccagaaacc tgcactattt ttgcgattta tcgtaatttt
    86941 gccactccag accaaacgga agcattgcgt agacgttatc tggctggagg tatgagctgg
    87001 aaagaagcaa aagaagagct atttaatgtt ttgaatgctc atctaacacc tttaagagag
    87061 agatataatg aacttatctc tcaacccgat gatataaaaa gaattctgga agaaggatct
    87121 gcccgtgcca gagaattggc tgctaaaaag ataaaggaat tgcgacaggt tatcggagta
    87181 gattaaattt aatctcctat ttcatattaa ttcaagataa taggaagggg actaatcgtc
    87241 atctaattca cccttgttga tttcccagag ttcatcatta tggatattgt aagtaaaatc
    87301 gtgacttcca gctttgacca gattctttaa tttatcggct attcgctcca tagtatctat
    87361 aaaatcaata gtatgtaagc cattaatggc atcacattct ccactttgaa tacaattcat
    87421 aatgtgttca cggagagaat tatgagtctc gttaattctg ccttctaatt caataatttt
    87481 atagatataa actggcttca gctcttccag atattcaatg cagtattcta acatttccat
    87541 aactttgcga taaaggtcac ctatcatttt tttcaggtca tcagatatgt tgttattatg
    87601 agaaggaatt tcttgattaa gaatacgatt agcttcttca gttaaatcac ctgctttttc
    87661 tatgtcatta acagtatgaa gtagagcagg gattttttga ataatctctt cggaattggt
    87721 gcgttcatta accgcaacaa gatagcgagt aatctctttt tgcaaattat caatagcaga
    87781 ttcaattctg tggatacttt cttgtttctt gtagttttta tccttgaatg cttcataaga
    87841 ggttcttaat tcaagcctaa ctaaacgaag catctctctc atttctttta aacattgttc
    87901 aattgcaagg taagaatcat caatcaaatg ataatcaaga aattttggtt ctcccataga
    87961 aatagtctct tccggaccct ggggtattat tttttgagca attttaacta atagtccaga
    88021 aaaaggtagc aaaattaaag cattaaagat attaaagaat gtgtgagcat tagcaatgtg
    88081 acgtgccaca ttttgatggg cataaacatt gccgggtgta atcaaattta taaatctgtt
    88141 aaaataacct aaataagtga gaatggcaaa gaacaatgaa ccaaaaacat taaaaagagt
    88201 atgaattaaa gccactcttt tagaagtaga gctggaagtt aaaactgcta accaagcagt
    88261 tatagttgtt cctatattac ttccataaac cagatataag gctccatcaa aagaaatcaa
    88321 atttgtattg gcaagtacca gaataatacc tacagcagca gaagaactct gaacaatcat
    88381 agtaaaaatc attcctgcca aaatagcaag caaaggataa tccgcagctt gagcaagaat
    88441 attccgagca aagagagaat tttttaagtc aataacggaa ttactcatta catttagtcc
    88501 tacaaagagc aatccaaaac cgagaatgat taagctccag tattccattt tacgactttt
    88561 tttgaaaaat agaccaaaaa ccccaagaat aacaaagaaa taggcaatgt ctccaatatt
    88621 aaaagcaatc agttgagcag taacagtagt accaatattt gctcccatta ttacgcctac
    88681 tgcttgttga agactcatta ttccagcatt aaccatccct atgagcataa ctgtggtagc
    88741 agagctgctt tgaataatag ctgttacacc aagtcctacc agcaatcctt taaaaggtgt
    88801 gccagtaatt cttttcagga tttgacgcat cttctttcca gctacagctt gaagattatc
    88861 gctcaacata cttatgccat ataggaaaag tgcgagtccg ccaaccaggt tcaaaacatc
    88921 tataaaagtc attttatcaa ttcccttttc aattgattta aagaataaat aattgtaaat
    88981 ttatttattt gcatttaatt ggaacttgag gaattaaata gattggatag atagttaaca
    89041 tttagattaa actgttcttc ttccagatgg tgcaaagaat gtccaaaaga agaacgtctt
    89101 tgattaaaac gtatcaaact acgagttagt gcatccttcc atattttagc attaagcggg
    89161 atgtgaattt gatagcgaga gataaattta tctaccaaat ttcttaattt atcagaagag
    89221 aattcagcac accagaaaag cagtaagtga gtaatatcat cttctacgac agaagtacga
    89281 gattcagaaa agtctattaa ccaaatttga ttccgggaat aaagaatatt ggaaggttga
    89341 ttatcccaat ggcagagata gaggttatcg gttctcattg ccttatggaa attgcccaag
    89401 gtatctgcta aaaggtctat tatttcagtt tgcggctgca catttaagta aggttttcct
    89461 tccacatatt caatttcaat ccatttaggt tcctcagagc aaagaagttt cgggaccatt
    89521 ggaagtcctt tttcatacac tttaaattcc ttccaatagc tatctgctga agaaaaatac
    89581 ttgataacag acttatctct aagttctata ctggtagtag aaatgcgaga aattctattt
    89641 atcaatcagc cttaaaccgt tttatttcat tatcctcaaa aagagggacc agaatctcat
    89701 tggtaggaag atgataatag atatctgctg gagctttcaa atctttttgc cagatgatgg
    89761 tgcgattctg ttcctgtgga atcataaaga tacatttttc tccccaggag ctgtaatata
    89821 tcctacccaa atcatcaata gctattccat ccagattatc ataaagagtt tctttgaaaa
    89881 tactaaatgt cttagtatca atgttaaaag caagaatagg agaagcaggt ttgaaacaga
    89941 caatgaacat ctgacgacga ggaccatcaa agactatgcc gttgggttta gtcataagag
    90001 gagagatgaa actatttatt tctttggtat gtggattgac gatgaataat ttattagctc
    90061 cggtatcagt gcaataaagc aaaccttctt tatcacattc aatatcattt agcatttttg
    90121 aaccgggtat aggaatactt tctacaatct ttgcctcttt tacatctatg atctggatta
    90181 ctgtgttatc tgcaacataa agagtgtctg ttctgaattt aattccccgg ggactggata
    90241 atccttcatt aatcaggact tcgtattgat taaaatctgt agtataaagg atttttccat
    90301 tacccgtatt ggaaattaga aatcttgaac ctgttccatc ccaggcaatg ctttcaggat
    90361 tatccaatga aggtggttga atcacactga ttataaaata aatagctatc attattacta
    90421 tagctacaat cactatagtt atagtcttct ttttgctttt tgccatcatt cctcctaact
    90481 ttttaaagtc caaatacaac cttcaggagt atcttttatc tcaataccta aactatttaa
    90541 atcgttacgg attttatctg ccagaaccca gtttttttct ttacgagcct gttctctata
    90601 gttaattaat aattgaatca attcttgaga gagatcagga agtgtttctt ttaatctttg
    90661 gcttaagttt tggaaaaagc ccaggacttt tcctaatttg accagcataa gagcagcttg
    90721 ttctcgttca gaacgagtaa gacttatatt tttgcattga tgagtaagtt caaaaagtac
    90781 agctactgcc tttgcagtat tcatatcatc atccattgcc tggataaaag cttgctcctg
    90841 ataagcacaa gaaccatcag gttggacttc aatatttaaa tagtccacac tttttaaagc
    90901 tgaatagaag ttatttactg cccgttctga ttcttctatg atttctttat taaaatctat
    90961 gggtgaatga taatgcttag aaaggaaaaa gaaacgaata gcctcagcac tgtattgacg
    91021 aaggatatct cttgttgtga agaaattatt caggctcttg ctcatttttt caccttcaat
    91081 attgagaaag ccattgtata accaatagtt ggctaaaggt tttccggaca ttgccagagc
    91141 ttgagcaagc tcgttttcat gatgaggaaa tagaaggtct attcctcctc cgtggatatc
    91201 aaatgtttct cccagatatt ttctgctcat aacaacgcat tcagtatgcc agcctggacg
    91261 tcctttaccc cagggacttt cccagaaagg ttcaccaggt ttactgcgtt tccataaagc
    91321 aaaatcagtt gggtgttttt ttaaagcatt ttcttctacc cgtgctcctg gaattaaatc
    91381 ttccatcttt ttaccagaaa gcataccata ttcaggaagt gcagtagtat caaaatagac
    91441 atcaccctca atttcataag caaacccttt ttgaatctgg agttggatag catcaataat
    91501 atctggaata acttctgtag ctttgggttg gtagcttgga ggtttaatcc ctaaggttgc
    91561 acaatcttcc tgaaaagcct ggatatactt agccgtaaat tcattaaagg gtatcccttc
    91621 ttcttgagca ccaattatta tcttatcatc tatatcggta atattttgta catagataac
    91681 cttataacca cggaattcaa gatagcgtcg tagcacatca aaagtgatga aagcacgagc
    91741 atttccaata tgaaagtagt tataaaccgt aggaccacaa gcatacatct taacttcacc
    91801 aggatttata ggtgtaaatt cttctttctg acgctttaac tgattataaa attgcatagt
    91861 gtacctttaa tgatatatta caagtttttc tattttttct gttttttcct ttccatcttt
    91921 agctcggatt ttaacaaaat aggtgttatt agccaattta tcacctttgt tatctcttcc
    91981 atcccaggga atagaattga atccttgcct gcccatagtt ttcatactat aaattttctt
    92041 tccggatata gtatatatat caatggtaag ttcacaatca cgaggaagca taaaagtgaa
    92101 gttggtggta gatttcatag gattagggta gattaaaaat cgttctatat aaagctctgc
    92161 cacttttcgc acttggaaat gggtggtagc aacagcagga agattgaaat tatcaaaggc
    92221 aataatctgt aaggaatgta ccccttcact taagccagat aaaggatagg ttaaaatgcc
    92281 ttgagtgaat gaatctgtat cataacggaa ataatttgtc actgaaacag gttgtaggga
    92341 attatccaaa atcaataaga tattatgtcc ggcactacct gtaacattta taccattact
    92401 atcagaaatt ctggcataga gaataggatc ggttccaact atatcacctg gtctaaaatc
    92461 catactatcc agataaattt ctatatgagg agcatcagga ttattagctg agacagcttc
    92521 atcacttagc tgaagaggat aataataatt agtgtaactt tgttttaagt tgggatccca
    92581 aaaataggac acaattaaac cactatttcc tgtagtaaca tcatcaggga cgataaaacc
    92641 actttgataa aaacctgtat taactgtgga acttcccaca aaaaggggag cacctctatg
    92701 actgataata gtttgaggat ctaagttgta aactaactta ttattgaaaa ccttaacttc
    92761 agctatacca ttagaggtac tgggagaaaa acttccggta aaacggactt tttgccgtgc
    92821 atataaaatc ccttggtttt cttttgtttt ataattatct gggtcaaaaa tggttaagat
    92881 actgtcccgg acaggtggga caatgtgaag caaagggtca cccaataaaa catagagtgc
    92941 atcatttata gtactttgag tataggcagt ttttgctgcc ataatggaat atcctaaggg
    93001 attacgtttg ttagccaaat agtctaatat aaaagtagtg agtcctacat tacttccagc
    93061 agcactcatt ctggttgcac ctaacgaggc aatagctcca agattattaa gaagcactgt
    93121 tttttgtcca aggctttcat atccccagta atcaaattta gatacttcac aggaagcagc
    93181 aataaagaaa gtaagtttgc ccggattatt aaaccttccc atatcggttg caccattgaa
    93241 ataatcttcc gccccgagtt ggtcatgacc tccatgtccc acatataacc agacaagtct
    93301 tccctcatta atagcagcca tcatatcatc acgagctttt ggtttgttct gaaattcatc
    93361 atattcatat tcccagccga agattttatc aatcaagata ctgggatgga tggtatttga
    93421 tagtgtttct gtctgctgag tatgataatt ttcataataa ggttcactac cgttataaag
    93481 gtcatctgcc acaaatacca ttgaattttt ccaccaacca ccaactggat tttcaacata
    93541 agagctaaaa ttagagaaca taatattaat ttcatttaga ttagtaacgg gatatctacc
    93601 aatagcaagc tcaggattat aagtttgagt taacataacg aagaaatcgt cggaagttga
    93661 attatctcgc tgataaacta tcattttatt ttttgattgg gcttggccag agaaattacg
    93721 ccaatcaata gttcccaaac ccagtaaggt tacagaagta atacgaggag tgggatagtt
    93781 ctggaataca taacttaaaa acaatctaat ggcttctgga tctggatgac cactattgaa
    93841 ttgattaaag atatcgcttt gtcttacaac cttgcttcgt ttattaaagt aaagattata
    93901 tttatctgct aaggtttgtg cctggtttat aaattcatct ggagttataa taatattatc
    93961 cacctgtcct ggattagata ccaaatcaac tggttcaata aattgaactt ttgcaggtaa
    94021 ataatattct tcattagtaa gaataaattt agttgctgta ttaccggagg ctatataata
    94081 gtatgaacca gcgatgtttt taactgggac aagagaagct tcgttaaaac tatttatctg
    94141 atatacagta aaattagtgc tactggaaac attatatcgg accgggacta catattcatt
    94201 ttctaactga tttacgatag tttgtttgcc agcatattta acaatattgc gggtgtattt
    94261 cacattaatc caatctaaga atatattatc tgttttatca cgatgtactc ttattcggat
    94321 agtatttact ccagggacga aataaggaac agatttttga aagacatatt cattcaaacc
    94381 ttgccagcta aatatatagg aatttgcaga agaaggaatg acattaccat ttacataaac
    94441 gcttatatta tgagtggcta tatagtttac agattcttgt cgcaatgaaa aacttagaat
    94501 tccattatca ttagtagata aatctggaag ttctattgta aaatcataat cagcagtaac
    94561 cttacctgct aacaatgtca tataccaatc aaaacctatg gtttctcttc tcaacttttc
    94621 ggtttccagg cgaacattat caacaaaggt tgaagtttgt gtgttccagg tttgtagctc
    94681 gggcaaagtg gcaatacgta aagggtcagt attgaattct cctccgaaag tcaaccagta
    94741 aacgcaattt cctgaataag gattgtaata agtgtcttta ttagattgaa gaatttgatt
    94801 tttctccact ccatctctgg tagtgccata aaataagata taatcctggg catcaaatct
    94861 tccatcttct tctccaatta cctggattgg aatttcctgg aaaggattgc caggactatt
    94921 gatagcaaaa ggtaaaagaa caccactatt gctgaaaagt ctgaaactac gaggatcaat
    94981 atcggataag ggaaagccag tcaattgaga aggagcaatt ttaaagatac cttctctatc
    95041 tgtttcaatc ttccaccagt aatccgaacg agaaaattct gcatattcaa ccttagctgg
    95101 agttatatat tgccagtttt tagcttgttc aggattgatt atttgagaaa ggaacaagtc
    95161 agttaaatca tcactttcca ttatacttcg gaaacttgta tttccgagaa tctctatttt
    95221 gattatagct tgatcggtta tttccagatt ataatttccg tcgtaggaaa agggatgtat
    95281 ttctaagggg caaaaaccaa tgcctctata agtggtggga ttaagtaaat tcagtaaggg
    95341 attattagat ttacttaatt gataaagctg ttcattgagg gtatattgat aagaagaaac
    95401 atcctgttcc atcaccactt ctggaactgg cattattctc ttttctaatt taactgaatg
    95461 atgacgggaa cttagtaaag tcacctgaat agaacctgaa ggcggaatag ctatcttggt
    95521 ttcttcatag gcaagcatag gtgcacctgg ttccatactg taattcatat ttgatgtaac
    95581 caggtaaacg aaatcatttt gtttggcaaa agaatattcg ccgaggttat attcaataat
    95641 gatttgttct tgcttggtct ctaaaaccct aaaagaggca ttggctacct gtagagccaa
    95701 cagaaagcaa aggatgagca gagtggtttt tttcattgtt ctttcttttg tttaaatact
    95761 atatttaaaa ttatgataag ggcatagaat acacccataa aaataaaaat ccctgccata
    95821 ccttcaacca gtattccaag agaatatttg aagttttcta ctacggtgac taaagcggtg
    95881 aatgtcattc ttgcctctca agttaataat gatagggtct attatggttt atcatctctg
    95941 ctctatagag ctgttctaat aaaatcaatc ttaccatttc atgggtaaaa gtcattgcag
    96001 acaatttaag agagtaatta gaaatttctt tgatactttg atgagttcca taagctcccc
    96061 ctataatgaa gactattttc ttgttttccg ataagttggc aaggaaattg gcaaattcta
    96121 atgaagtttg ttcttctccc tgttcatcca gcaaaataac ataatcatct gaattaattc
    96181 ttttcagaca tttagaggct tcttttgcct ttaccatatc ttgggaatca gcattaagaa
    96241 tactcactga gggaatctct tccacctcta tcttcaaata tggttccaga cgggtgagat
    96301 acatttgtat tccttgctgg atccactttt ctttggtttt tccaatttgt agtatcttta
    96361 tcttcactct tgcatacagg ttaaagccat tgccatacag agcattttca attcgcctgg
    96421 atctgctcgg gaaggtataa gaattggtgc tttagtgccg actataacat gggcgactct
    96481 atatccacag taataagtga gtaacttacc aaatatattt cccgcttcta tattaggaac
    96541 aataaataca tcagcatttc ctgctactgg tgattctatt ccttttattt ttgcagcttc
    96601 aaggcttaca gcattatcaa aggctaaggg tccttccaca atacaaccag taatctggtt
    96661 tcttttgttc atctgtgaaa ttattgcagc atcaagggtt gactgcattt taggattaac
    96721 tgtttcaaca gcagctaaga gagctacttt gggctgagat gaacccatac agtgagcaac
    96781 ttcaacggca ttttttacga ttgctatctt ttcttctata tttggagcta tgtttactcc
    96841 accatcgctt aataatttca ctccctcagg atgctcataa gcaagcacat ctgatataac
    96901 aggactgata cgaagcccct tttctttatc taaaacacag tgtaataaaa gtcctgaatc
    96961 agtttttcct ttaagaataa tatccccttt cccttcatta accaattgta cactcatttc
    97021 agcagctttt actaagtcac ttcctacatc aactatttca aaactatagt ggtgattagg
    97081 gtctaacttg cccaacaaag aaagaatcaa ggatttatcg cctaccagta agcagtctgc
    97141 cagattttct tcttgagcca taatcgctgc ttgtagaact gattccgttt gagcagctgc
    97201 aataataaca gtacctcgtc ttttagcttt aacgcttgct accatttcat tgaagttttt
    97261 aaacatatac ttgttcctat tcttgcttag actttagggt ttttaatagc tatatttttc
    97321 cagaaattca tctaccagct tatcttcttc aggttgtaat tcagctaata tttttttcag
    97381 agcttctttc tggatttggc gcactctctc gcgtgaaagt cccatagttt cagcgatttg
    97441 ggcaaaattg cgggtttcat gattctcatt taaaccatag taggttctaa tgatatccgc
    97501 ttctcgcttg tccaatttct ttatagcatt ctcaatttta ttttgtaacc gttcacgact
    97561 gtatatttct tgcgggtcta ccaatccttc atctttgatt aaatcacgag ttgtaaattc
    97621 ctgaaagtca ccattttgta aaagctcatc ataagaagtt gtctcaacaa tttggtcttt
    97681 tagctgctca atagattttt ccggtagatt cattctttcg ctaagttcta tgatggaggg
    97741 agtttctcct gtttcagaat agattcggtc ttcagtagct ttaacgcgat gggcggaact
    97801 tgattttccc ataggaacgc gaataagaga acttttttca gaaactgcct gcataattct
    97861 ttgtctaatc caccagatgg cataggagat gagttttatg tcttggtcag ggtcaaattt
    97921 ttcaatggct ttgagtaaac ctatattacc ctcactgatt agttcagaaa gtgaaagtcc
    97981 tctattttga tactgagaag ctatttttac cacaaatttc agattagcct gcatcaattt
    98041 gttaatggct tcttgatcac cttctcgtgc acgcagagct aattcgtgct cttcttcacg
    98101 actgagtgtt ttaaatttgg atatctcttt caggtaattc tgaagagctt tatccgcaaa
    98161 aacattttcc ctattcataa actcctattc tattttttaa aagggagcac cttacgtgga
    98221 ttgatagcat tgcctttgac acgatattca aagtgcaaat gagatccagt ggcattgcca
    98281 gttgctccca tagttgctat ctgttgacct tgtacgacat tatctccttc acgaactaaa
    98341 tttatctcat tatgggcata aacagtcata ataaaatcag gatgttctaa aataatcaca
    98401 tttccgtaac cattttgtct tccagcaaag agaacaacac catccattgc agcatagatt
    98461 ggagtgccag ttttattggc tatatcaatt cctttatgag gtctgccacg tcttaatcca
    98521 aactcagagg agacatatcc ttctgcgggc actatacagt tagtattaat ttgttcactt
    98581 tttaatgttc cagaacttga gggtaacttg agttcttcct cttcctcttc ttccagagtt
    98641 cttggttttc ccactaacca gattttctgt cccacagaaa gtttatccga ttttagatta
    98701 ttcaaagcct ttaattcttc tactgtcatt ccattttctc tggctatttt atataaagta
    98761 tcttttttct ggacaacata gattttttca ataattaaag aatccgggtc ttcgggagta
    98821 ttagtttcta ttttgggtgc atctaaagtt gtctttttct gatacaagga gggatcctta
    98881 ataaaaagtc tgtctccttc ttttatggta tgagattcat tttcaaagtt attccatttt
    98941 aataaatctg caagtttaat cttattatta agagcgatac gataaagatt atcacctttc
    99001 cgcactttat agtaataatc ttcaggaaag acgatagatt ctttatccgc taattctgcc
    99061 ttttgtatta tcggagtggt tggagaatct ttatcctgaa tagtgctaag ggaaggttta
    99121 gctgggataa ttgctggtac ttcaggagtt ttagtttttt cttcctcttt tattttaagt
    99181 acctgacctt catgaatgat atttccctta agcccattta atttctgaat ctgttcaata
    99241 ggtacatcat attttttact taagctataa agggtatcct tgggttggac tttatgtgtg
    99301 atatattcgg ctgataaaat aaaaggtaat aaaagaaaga gcaggaaaag agtcattttt
    99361 cctggtggtc ttatatctaa aaacttatct ttctcttctc tatctaatcg cttataatat
    99421 ttgaaaatca ttatacatta ccgtatcttc ctgttctgta atttcgagtt cacgaacatc
    99481 tatgaatcgg ttagcattac gaagtagttc gcaaaatcgg ttcaagcttt cctggtctcc
    99541 cgtggcttct atcaaaacac taccatcata taggtttcta acatatccag taattccaca
    99601 gcgtctggca caggtctgag catagtgtct aaatcctact ccttgaactc taccccgtgc
    99661 aattatcttc caggaatgca taatcaactc ctttcaaaga tataactttc tatttcccct
    99721 aaggatattt gtcaagcatt catttcttct caggtaattt atctatttga aatacaatat
    99781 attatgccaa tagtttccaa attccataaa tatgttttag cacaccttct attatgattt
    99841 gcctttgaat aaagaatatt gccttaatat ctggtaagct actcagaaat tggaacctgg
    99901 gattatacta aggattacca aatatccttt atttaatcat aattaccttt cttatgctat
    99961 tctttttgcc ctgacttgct tttatactat agataccact actaactgtt ttaccattat
   100021 tatctgtacc atcccattca tagataagcg attttggact gtaaccttta tagatagtct
   100081 taaccagttg acctcttaaa ttataaactt ccactttaac aggttggtct gcgtttaacc
   100141 attctatgcg gaaagaagtc ttttctccaa agggattagg ataattggca aagataagga
   100201 ttttattgtc cccagttaaa tcagtgctta tgccaggttc attaaaacag tgatggaagc
   100261 tatattccac caatttttga gcctcagttt tgttcatatt gtaaagaggg aaacttaaga
   100321 ctattacctt accattctga ttaggattat agattcccac agtgcttcca tttaaagagc
   100381 cctgaggagt ttctgaatca taatcggaac cgtataagta aatcgcatta gcataaggag
   100441 aaggttctat gctttcaatc tgataaatat gtccgttaaa ggaactgata gttttttccg
   100501 ggtctacttc cagagggggg aaatcttcaa agcagggtat agcgtatttg aatctggctg
   100561 cgctggaata atcagtttca cttattccaa tattctgata aatgaaagta tcagaattaa
   100621 aatagttagg ataagaagag ttcaaagcaa aggctaaaga aggatgatag acactaaaga
   100681 aaagattacc tccataatct atatatttct tcaaatcatc ttgaatgaaa taaggatagt
   100741 ccatactggc aaggtcatta ccataccata ggatagatga ataaattccg atgtcagcca
   100801 aagtcaaagt ttctgagagc tcgtttaaat ccagttgatg tgtttcaaaa ttttgcataa
   100861 tatcggcata gaagatatct gtttgttcat cagaaggttg gaagggatta ttaccactca
   100921 tattttctgt ttcatctaca ataagaattc ctttatttaa agtaaccggg cgagttttta
   100981 tggctgaagt aaattcgctt ctattgccat cctggtctat agcgcagagt cggtaataat
   101041 aataattttc tgagcctatc acatctctat ccagaaagct attttgagta agtaaaccac
   101101 caatttgaat acctaattct ccttcagatt gagagcgata tagattatag ccagccaggt
   101161 ctaattccag attttcattc caggataaag aaactgcacg gtatacaggt tcaactttaa
   101221 aattctcagg agttagtgga attaaattag gagttcccgt ggcaactatt ataaagcttt
   101281 ctaacccatt aaaatctaca ctggagactc ctacataata catttgccct tcagtcaaat
   101341 tatccaatat ataagaagtt tgggttgtag aaatgggctc gttccattgt ccaaaaacag
   101401 ttgaataata aaggttataa tggtctaaca caggttcata aggactttcc caggtgacaa
   101461 gtaaggaaga accatttccg gtatcccgaa ctactaaatt tatgggtgct atggggattt
   101521 cagaaaatct tacagcagaa gcagtggatg ccttaattac ttcagcacag taatctgaat
   101581 taatatgttg tgtcagatcg ttggtggtat gataataagg agaaaaatca aattccgaaa
   101641 aataaaggac attatatccc ttttgccaga aggaatagct atcactatta gagggattta
   101701 ctgagtaatt gtaataaact tgtaaatcag tatactgttc aattaattgc agagcataat
   101761 tggtataatt ttctgagcca tcatagggat ttaagattac ccggtaaaag taagggtcat
   101821 tattattgct aatcatatca tgattaatca tcaaacggat attttcatta ttattaaggg
   101881 cagttaaagc ataatattta gaaccccaaa gaccaaattc ttctgcagca aaagtaacaa
   101941 aacggataga acatctgggt tgataaccac tttgcataat cacccgggca atttcaagag
   102001 ttcctccaga accgctggca ttatcatctg ctccgggagc aaatacaaat ggattttcac
   102061 ttggaccgta ggtgatagaa tcgtggtgtc ctcctatgat tatatattgt tcaggatata
   102121 gagagcctgg aatggttgct accacattat actggtcggt attgttccag atgaaagatt
   102181 gtaattgcac atcatttatt ccgaagctga taaatctatt ttttatccat tctgcgacct
   102241 gaaacctgtt atcagccagg gcaaagcgag tctgaaaatc ctgcaaacac tggatattat
   102301 aaagaataga atccgcatct accaaagata taatttgagc tatatcagtt ctaaaagttt
   102361 ctcttttttc gggtaacaac ttaatatcag aaaagattat cggttctgga cctaaatgag
   102421 taaagggatg tttaattgtc tctcggagta ttatctcatc cagggaactc tgaattagat
   102481 aagcagagcg caagttaaga agtattgttc ctacagatga aggcaaagaa aaagactcct
   102541 ccgggatttt tgaaactaag tataaatctg gtaaaataga gatatcccca atttttaagg
   102601 caccagggaa atcttttgct ttatctgatt caattataat cgcatattca ctatcataat
   102661 gtagaatttg aacaccctgt tgagaaagct gttgcatagc ttcataagta gagagagcgg
   102721 tgtagcggtt cattatttgc tggacgggaa tggctaaaat tgagggtaaa gccatcaaac
   102781 tactcgccag tagtagtgtg gctaaaaaga taacccattt tttcattttt atttccttgt
   102841 atatataaga ttttacttta gattataaac tttaggatat gattagtttt gattatagaa
   102901 aagtttttcc tgaaatctgg caaggaattt cttaatcata attgttcttg actttaataa
   102961 tcctaatttt tatcaagatt ttaatatatg gacattatga aagaggtaaa aataatgaaa
   103021 attagattag tatcacttgt aataatttcg gtattgttta gcgttttgat ggctgaaaat
   103081 ccacagaata tcaatctgga aatgaaaatg ataggcttat ccgatgttcc atccgaggaa
   103141 gaggtatatt tgggagttta tattcaagac ctggattatc caactgctca agcacttgga
   103201 tataatgaac tttatggcat tctgataact aaagtggaaa aaggttcacc tgcttggaat
   103261 atagggatta gaaagaatga tattttaatg gaaattaacg gatatcctat ttttaatgtt
   103321 aaagaatttg aaagtattaa aaaactttta caccccggag atattatatg gcttcatatc
   103381 tggagagatg gaaatatatt tgatattaca atgaaagtac agggaagaac gaaagagaaa
   103441 agtgatgcca aaaaacaacc taaaagtaag gaaagttatc cagttggttg gggtggtgga
   103501 agctggatac cttactgggt agtttttcct gttgatgatg tcaatgaact aattgctcat
   103561 ataggtgaac cgacaggaga taagaccttc gcaaataatg ctattagtga agatggggta
   103621 tttatgagtg gaggtggagg taaaataaac ataggaatag gatttttcct gggtggggtt
   103681 ggtgcaagct atagcttttc cgatagtgat gcgagcacgc attctaaaat taaatataac
   103741 ctttcctttg gtggaataac tctggacaaa cgatatgctt ttaatagaaa actgattggt
   103801 tctttgggca ttatgcttgg ctcaggaggt catcaagtgg aatattctgc tctggcaaat
   103861 accgatttca attggcccga gatctcctca agcggaagtt tcaatgtaaa agtctcacgg
   103921 gaatatttta tgattcaacc ccgggcagaa atgctaataa gacttttacc ctggcttgct
   103981 attcaaacgg aagtcgggta tatgtacggc atcccaactc atagtggatg gaaaatgcaa
   104041 gctactacag gagatattta tagcattgat aattcaccag atacaccttt tcatagtttg
   104101 accataag
//
LOCUS       DAORZF010000002        87472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_214_length_87472_cov_402.975886, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000002 DAORZF010000000
VERSION     DAORZF010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 87472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 87472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87472
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_214_length_87472_cov_402.975886"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..1508
                     /gene="rmuC"
                     /locus_tag="PK015_00460"
     CDS             66..1508
                     /gene="rmuC"
                     /locus_tag="PK015_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOL32975.1"
                     /translation="MSNIEVIILILCSLLFIIGIILIILLSRKNLPMEQLKDSLNHIS
                     TLIESFERTIRDDIQRLRADLLSFQSNTRTELLTTLNQQNEAISKENRSNREEISSSL
                     AKLNQQMNEDAAKNRNELGNSLRDLSESLSRRLQELVSTEQTQFDSLKTAMEAKMEQI
                     RSSNENKLEEMRKTVDEKLQDTLEKRLSASFQQVSERLEQVYKGLGEMQNLANGVGDL
                     KKVLSNVKIRGMQGEYQLEAILEQILSPGQYVKNWKPDKNKNEVVEFAICIPGKEDPK
                     EKVYLPIDAKFPLSDYNALVDAWDKGDLAAVENARAALRDRILKCAKDIQKKYIAPPL
                     TTDFALLFLPIEGLYAEVLHDPTLFDELQKYHIVAVGPTTISAILNSLQLGFRTLAIQ
                     QRSNEVWKILGAIKTDFAKFGDLLDKTQKKLQEASNTIEDASSRSRQIQKKLKSVEEL
                     PLEESSVILELGEEKDYQVQVEENTDEDLS"
     gene            1571..2545
                     /locus_tag="PK015_00465"
     CDS             1571..2545
                     /locus_tag="PK015_00465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOL32976.1"
                     /translation="MFLTGQQMKEVFLKAKKQRFGIIASNVVFDMQVRALVQGYNAVK
                     SDGLLQMSSGACKYAAGETQDIKAGARLISAIAKVFTEQFPHCGVGLHIDHATPNYFD
                     FIVYCIENNLVSSVMIDASKESLEENIRITKEVVDIAHRHGIIVEGEIGYIKGSEDEI
                     VSETELYTKPEEALEFVKKTNVDLFAASVGTNHGVTKGENVVLRLDLIKEIDDLLIAN
                     GVERGLVLHGASGLTDEQQQTAIANGVVKLNKDTHYQMDMAAAVQAYWEKEKDAIVCP
                     PDVPPDVYMPDKSRFDPRKWLIKAEQRMQNTVQSFCLMSGSANNSILL"
     gene            2562..3560
                     /gene="gap"
                     /locus_tag="PK015_00470"
     CDS             2562..3560
                     /gene="gap"
                     /locus_tag="PK015_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOL32977.1"
                     /translation="MKKVAINGFGRIGRLVFRAIIKYHPEMEIVAINDITDAKTLAYL
                     LKYDSVHKIFDANISNTEDSIIVNGKTVRVFAEKDPQNLPWKDLGVDFVVESTGVFNT
                     RESAAKHLTAGASKVILTAPAKDTVDATIVMGVNHNILTAEHKVISNASCTTNCLAPI
                     AKVLQDNFGIINGLMTTVHAYTNDQRILDLAHKDLRRARAASLNMIPTSTGAAKAIGL
                     VIPELAGKLDGIAIRVPVPDGSLVDLSANLEREVSREEINAAMKEASETYLKGYLQYS
                     EDPIVSSDIVGNSSSSIFDATSTFVKGKLVKVFSWYDNEWGYSCRVADLMEYLSRL"
     gene            3609..4376
                     /locus_tag="PK015_00475"
     CDS             3609..4376
                     /locus_tag="PK015_00475"
                     /inference="COORDINATES: protein motif:HMM:NF025597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HOL32978.1"
                     /translation="MARYFYTDKGKTLGPVNREQILNLILKDVLSLDSLVMDTRSPRW
                     IKIRDIPELMHFLYESDIQISDWAEEIALSGESDESIPLFFHIPTARLITLSIVTFGW
                     YELYWLYMNWRFLHYFRKNSSAKAFWLDLINPLGVIRIFQHISNDKELRVQKEERDFT
                     VNGIIWILALLTLFIFASGIINYLTRIPFLVSIFVSFFALTLSLCCIMPIQNYINTAN
                     MRFNKKLSAHTFGHYGTIILGILTWIYVLILWIPALS"
     gene            complement(4379..5212)
                     /locus_tag="PK015_00480"
     CDS             complement(4379..5212)
                     /locus_tag="PK015_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL32979.1"
                     /translation="MKKKMLPLLIIMLFLLVGCVEYNEEMWLNPDGSGKVSLRLVHRS
                     NYANTQEIMRKSELPGIHLISSQVQKNGPFMVYKVTFKFDNIESFNRISEEPTPVDFL
                     GQISINKNSEGHLVYKRRISLGGSEILEPGTESDSIDINLLSDPDDILASIYKKQQKD
                     YPKWNYTIHLPWKIISASTTADKIDYKNKTVSWEFDTEQMWNKSELMTVEMKKGISWI
                     VWVIIALAVLLLAFFINWLIKIRHRSHLQEAIEHNQRPENQQSPPTINLEESGEEKDL
                     K"
     gene            5520..5897
                     /locus_tag="PK015_00485"
     CDS             5520..5897
                     /locus_tag="PK015_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HOL32980.1"
                     /translation="MIELRQLYHCTVCGNVVEVTYVGGGELVCCGKPMELLVGNTVDA
                     ATEKHVPVLIDLGDKVKVTVGSVPHPMEEKHYITMIEVLTEKKVLRRELKPGDAPEAT
                     FCVKAEKVIGVREYCNIHGLWKA"
     gene            5925..6515
                     /locus_tag="PK015_00490"
     CDS             5925..6515
                     /locus_tag="PK015_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOL32981.1"
                     /translation="MAFKDSRTADNLMKSFAGEAQARLRYQYAARVAEKEGFIQIRNI
                     FNETADNEKEHAKLFIKHLIANGLEGEARNIMASYPIGWSPDSTLKNLLYAANGEKEE
                     WSELYPLFADIAEEEGYTDIANTWRQVMKVEQQHEKRYRKLYENVKNQMVFKRNEKVY
                     WKCNNCGYIHEGTEAPATCPCCQHPQAYFEVLCENY"
     gene            6527..6667
                     /locus_tag="PK015_00495"
     CDS             6527..6667
                     /locus_tag="PK015_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOL32982.1"
                     /translation="MQKYQCIACGYIYDPAENDDIPFEQLPDDWTCPECGVGKDMFEP
                     ID"
     gene            6726..7172
                     /locus_tag="PK015_00500"
     CDS             6726..7172
                     /locus_tag="PK015_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOL32983.1"
                     /translation="MTAQEFQEIIDFAIAREEESIKFYQDLQKETKFQGQIEMLKELE
                     AMEKAHKSTLQNIRYTNDFNIDKVPNLHISEYLTDDIEKLDLSYPNLLVMAMKREEIS
                     LRLYSELSVRFADEQIGNLFRKLASEEAKHKLWFETLYDEWLKQGN"
     gene            7177..7848
                     /gene="tsaB"
                     /locus_tag="PK015_00505"
     CDS             7177..7848
                     /gene="tsaB"
                     /locus_tag="PK015_00505"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOL32984.1"
                     /translation="MKLALDTSQSSGSIALWDKGKTIWSACFDIRITHSETLMPQIDT
                     AMKFCGYKPADIKTVLVTSGPGSFTGLRIGLATAKGIAYALKIPILTYSSLQLYALQR
                     YHCGKDILVLLDAKMQEVYAALYNENLEEIYPPQVCTLEHILSWEIRDCYLMERGINS
                     LELLSQEKNVPFISVPDLPISAAGLFLLQEIFPQPEIYNLDYIANLEPKYLREYNAQI
                     QKHKN"
     gene            8012..9340
                     /gene="ssnA"
                     /locus_tag="PK015_00510"
     CDS             8012..9340
                     /gene="ssnA"
                     /locus_tag="PK015_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HOL32985.1"
                     /translation="MQPYLIYGGIILTFNPELPLLEGYSLLVEDGKISQILKDTEIES
                     LPYERLDARGKIVMPGLINAHHHFYSTLVTGLTKASPAKDFLGVLENLWWRLDKQLTA
                     EDIYISALISAMNAIRNGCTTIFDHHSSPGFIKGSLSQIAKAIQESGIRASLCYEVSD
                     RDGINKCEEGILENVTWLQQVSQEQNPRLKGLFGMHAAFTLSDDSLKDIADWVDKLNC
                     GIHIHVAEGEYDELFNIQHYGQRVIERLCDFDLLNCHSLLAHGVHLNAREMMLIEEAG
                     AAIITNPQSNLNNAVGIADICKMNELGITVGLGTDAMTTNMLEELRVGIWAQHLKQNN
                     PSAGFEEMTKTLLLNNPIIAQKYWGDNFGFIAEGAPADIILIDYEPHTPLNEENWIGH
                     IVNGISQANVNTTICAGEILMWNGQLLLSVDENEVRKRGRELAKALWERF"
     gene            9350..10543
                     /locus_tag="PK015_00515"
     CDS             9350..10543
                     /locus_tag="PK015_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HOL32986.1"
                     /translation="MYHEILEKAREYEAESIRYLTDMIRIPAFSTKEKELAEYILQEM
                     KKAGFDEAFIDPLGNVIGKIGSGKNIIAFDAHIDTVYPGDLSLWDFNPFEPQVKDGMI
                     WGRGAVDQEGGMASMLTAARIIKELNLNSKFSIYFTGTVMEEDCDGLCWQYIIREDKI
                     VPELVVLTEPTNMNIYRGHRGRMEMQVFVQGRSCHASAPERGVNAIYKISRIALEIEK
                     LNEHLRYDDFLGKGTIAATEVHFTGPSQCAIPDSAYLHLDRRLTQGENKEIAVEEVKE
                     ACQLAGESDARIQVLTYNEPSYTGLVYPTEKYYPTWVTPADSPYLKIAERAYSQTLGR
                     KPVIDKWTFSTNGVAIAGMHHIPCIGLGPGNEVYAHAPNEATPVIHLTEAAAFYAALI
                     YALNQ"
     gene            10540..11697
                     /gene="thrC"
                     /locus_tag="PK015_00520"
     CDS             10540..11697
                     /gene="thrC"
                     /locus_tag="PK015_00520"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOL32987.1"
                     /translation="MSFFQNWRCTNCGNEYELDEVHYLCPQCSKKWIGGVPLIGVLEA
                     FFDYQAIKQEWDKQPDPGVFSVLEEKFYPPLPVGNTPFFRPERLNKNLNLPNLFIKND
                     ALNPSGSLKDRASQLVVADAIRLGVDKIVCASTGNAASSLASICAAAGKEAIIFVPKS
                     IPEAKLIQIKVHKAKLYTVSGTYDDAFNSALAYSANNDCLNRNTAFNPLTIEGKKSAG
                     IEIFLQLNDVPDWIIIPVGDGVILGGIYKAFKDLKSAGIIEKLPKLLSVQAESSDAIT
                     SYWETGSYHNANHPYTIADSISVKTPANAHWAVNALYQTQGKSVRVSDEEILSDQKYL
                     AQMTGIFAEPSSAAVFSGLKKALTQKWIKPEEKIVLLITGTGLKDINAVRL"
     gene            11681..12658
                     /locus_tag="PK015_00525"
     CDS             11681..12658
                     /locus_tag="PK015_00525"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase family protein"
                     /protein_id="HOL32988.1"
                     /translation="MPLDYKPAGICDFHVHIGEKIGGYELRDDFPDLDRIAELNGIAG
                     IGAFVTEESDLSLTVKLRKMQEKARLNFKGKVHWHLTPVHTKTDELFPLLQEGWDLKF
                     YTTYRESGLYCSYEQIEHWMIELSDIKTRMLVHCEDDQIITQASAEHPFRTPQDHTLR
                     RPELAEIVAVEKILNLAIKHTHPIHIVHISSPKAAILVNEAKKDYPFITCETAPHYLI
                     YNENRLSGINAHRWLCTPPFRSEESRGLLVELLQDGYFDILASDHCPFKLEDKDRFKD
                     NLELVPCGIPGLETLYSSMFNNFVEPGIISQASLDEMTIHKPKTLMSCF"
     assembly_gap    12670..12769
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12770..14963
                     /locus_tag="PK015_00530"
     CDS             <12770..14963
                     /locus_tag="PK015_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HOL32989.1"
                     /translation="GQAIYTDDLKLHDMLYAVPVYSELPRAKLISLDGSAALKQSGVV
                     AIITAKDIPGNIRYGQIIKDYPVLVDEDINSTGDVLALIVAHTREQAITAVPFIKIEL
                     QPLKPILDPEEAMKPDAEILHPFHGSNLTNYHRVRRGDVATAEKEADLIIEEEFSTSR
                     VEHSYLEPESAICHLRPDGVMEVFGSMQHPFSTRRFVASTLGVELKDVEVKPLTCGGG
                     FGGKDDTAALVCARAALCAWILKKPVKITYSREWSMRESYKRHPYKMQYRMGLNKEGQ
                     ILSVKARIVADGGAYCSVTPWVTWRSTVQSCGCYEVPNVETDVYGVYTNNVFCGAMRG
                     FGSPQINFAIEQLVEIAAKKLGMDEITFRRKNMVKQGSTTITGQVLDNHIVSLTQVLD
                     AVLKESDYENKRKLCSLGNPNQKEWYGIGLAISYRGVSLGAEGTDVNSAILYVQPDGS
                     TLLETGVFENGQGAESAMILIASEQLGLPLERIRYRMPSTSNIPDGGSTVASRGTIMG
                     GGAVVNASIILKQIITETIQKITAKSVAHFKDSKLLDSSDNLIMSWDEAIKLCYDNQV
                     YPYAFGVFQPPQISWDEETGQGNAYFTWVYGCQAVELKVNPQTGAVELLNMIAAHDIG
                     KVINPYALKGQYYGGMAMGAGYALFENCPCENGLVVPTNFHNYRPPRSINLPEMKAIF
                     IENPDPISPSGAKGIGEPANEIMAPAIANAIYHATGKRYHSLPIRIKI"
     gene            14960..15418
                     /locus_tag="PK015_00535"
     CDS             14960..15418
                     /locus_tag="PK015_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOL32990.1"
                     /translation="MITVNGFNYYNYDPERKLSLWLREDLSLTGTKIGCDIGVCGSCT
                     VLVDGEPKLSCRLKLKDVENCNIITIEGITLPDGTLHPVQQAFIDAGAIQCGFCTPGM
                     VLTAYALLNKNPHPTRQEVRLAIRGNLCRCTGYQQIIDAVMLAADRLSSN"
     gene            15445..16626
                     /gene="ygeW"
                     /locus_tag="PK015_00540"
     CDS             15445..16626
                     /gene="ygeW"
                     /locus_tag="PK015_00540"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HOL32991.1"
                     /translation="MDKKEIIKSRLLELEKLQPELYGKDFLLTWENSLDNLKAIMAVA
                     ELMQGLHWEKKPFRIFDYGLAVSIFRDNSTRTRFSFASAVNALGLALAELDEVKSQIA
                     HGETVRETANMISFLTEVIGIRDDMYPGEGHSYMLEVAEAVNDGFQNGVLAQRPMILN
                     LQSDLDHPTQCLADLLKLKSYFGDLNQLKGKKIAMSWAYSPSYGKPLSVPQGIINLMS
                     RFGMEVVLAYPEGYDLLPETVNSAQAFAEQSGGSFRITHSMTDAFTDADVVYPKSWAP
                     MTVMQERTRLLKEGNLQALQALEKQCLAENARHQDWECTEEMMKLTRNGNALYEHCLP
                     ADISGVSCPKGEVEKSVFERYRIHTYQEAGYKPFVIAAMIFLSKIKNPLSHLKSLNIL
                     E"
     gene            16629..18077
                     /locus_tag="PK015_00545"
     CDS             16629..18077
                     /locus_tag="PK015_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOL32992.1"
                     /translation="MPKLITEKNYNIILKNAKRCKERGIILPTIKQQINPETIPNNIK
                     NQLSNIGLWDINPLNLFRITWKNDIKTGLFGKPNFFELPSSLTGVPARIIGMVGKYFP
                     TGAHKVGAAYGCLVPRLVSGTFDPETQKAVWPSTGNYCRGGAFDCALLACPAVAILPE
                     EMSQERFEWLRQIGAEVYATPGCESNVKEIYDLCAELRKDPRYVILNQFEEFGNPFWH
                     YHVTGNAIKEVWDIVKDEHSNFSGFVSATGSAGTIAAGDYLKKHFPLMKTIAAEALEC
                     PTLLFNGFGGHRIEGIGDKHIPWVHNVRNTDAVAAIRDEDCLRLFRLFNEPSGQEFLI
                     KEGIAEDIVQKLPLLGISSIANLLASIKLCKYFELGKDDIIFTVFTDSAELYQSRLIE
                     QRNLKGEYSERQAALDWEGTIMSQQIDYFLELRYWDKKRIHNLKYYTWIEQQGKTYQE
                     ILQQWDPAYWQETFEDNLEQLDNAIEEFNALL"
     gene            18064..18582
                     /locus_tag="PK015_00550"
     CDS             18064..18582
                     /locus_tag="PK015_00550"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOL32993.1"
                     /translation="MPFSDILTIIPASGKGKRFGQPKHKALLNGKTFTDCIINTLKEA
                     EINNIYLAKDFNTKDMLSTLRCALKQLNLHRFSGFLIFPIDHPFVQSSTINALCSTFH
                     QFPEMVIRPSCNGKTGHPVLIPSSLDLFIDDNEEGLASIIRTQAKGIIDLPVEDKGIL
                     LNINRPENLLWT"
     gene            18573..19136
                     /locus_tag="PK015_00555"
     CDS             18573..19136
                     /locus_tag="PK015_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOL32994.1"
                     /translation="MDLMELIATRHSVRDYLPDPIPEEILKQILEAGRLAPSARNRQP
                     WRYIVFTSNDDIKELSLRCGLIGLSNIFIRKAPCLIVACADTNYNVRINHQDYYLVDT
                     AISVQQMVLTAWSFGIGSCWIAAFSEKTLKKYLQLPDSWRIVALLPLGYPAEKKTLYT
                     QALSVFPKSKNRLSLDKIVTYYHKSSS"
     gene            19210..20289
                     /gene="add"
                     /locus_tag="PK015_00560"
     CDS             19210..20289
                     /gene="add"
                     /locus_tag="PK015_00560"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HOL32995.1"
                     /translation="MNIKLTREFVQKLPKTDLHVHLDGSVRINTILDLAKKFNIQLPT
                     MDPGELRKLIVCDENTRSLEEYLRGFDIVNLVLQTKEGLYRAAYELAEDAANENVRYM
                     EVRFSPILHTKKGLKMTEITQSVIDGLRQAEREFPIETGVIICGLRNMDPTTSIKLAE
                     LAVAFKNRGVVGFDLAGSEFNYPAKEHKEAFELSLKNNLNITIHAGEAYGPDSIHQAV
                     HYCGAHRIGHGTRLVEDGDLLNYVNDHRIPLEICIKSNFHTKAVPNIQSHPIDFYIDY
                     GLRVTINTDNRTISDTTVTDEYMLAINELHLDYPTIKNVILNGFKSAFLPYKERVRLI
                     NETLRELEELEKSELKTTIKMKENL"
     gene            20292..20870
                     /locus_tag="PK015_00565"
     CDS             20292..20870
                     /locus_tag="PK015_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOL32996.1"
                     /translation="MFDLIQKSFTEAEKTLQAFITNNHNLETIDKIADLIAEAFERDN
                     KVIIFGNGGSMCDAMHFAEELAGHFRQNRKALPAIALSDPSFITCIANDFGFEEIFAR
                     GVEAYAQAGDIVVGISTSGNSLNVIKGLQKALSLDCFTVALLGGNGGKIAGTCDFELI
                     VPSFATDRVQEIHTMIIHILLENIENRLFAYI"
     gene            20884..21918
                     /locus_tag="PK015_00570"
     CDS             20884..21918
                     /locus_tag="PK015_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOL32997.1"
                     /translation="MQKIIFTLILISLSIFCFAQGNYYDSVIDLTGQTLYTALHSLIS
                     TNTNNSYNGSKAFLFQQLDNVNGYVTCIYTGQSFYVGYNYTGSTNPNTEHIYAQSWFG
                     VENDPVRRSDLHHLFPSDSNVNSARSNYPLFTVANHNSANVYYSYTPWQSYRGLSASG
                     YTVFEPADESKGNVARALLYFNTRYYDTLTQQNVNMIPDLVVWNTQDPPDAAEIARNN
                     AIYEFQNNRNPYVDHPEFVDRIWSTPITDDAITSPPSYLSLSSISPNPFHNTAVINIK
                     SQDVGKAEISIYNLKGQKIRTWNQTLLSGSTEIIWDGTNSSGTKVPSGIYLINIKSGG
                     ETITAKVLFN"
     gene            21949..23985
                     /locus_tag="PK015_00575"
     CDS             21949..23985
                     /locus_tag="PK015_00575"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOL32998.1"
                     /translation="MSLNYEQIKSQLQSYKPKWESKKTQLEALTIPEDFPFKEFFNAS
                     QDIFLQGYEFGKIISEDPEFKSTPIELLQTLNADYFAPIKPEGYQRSLANPDYTVNLY
                     GKDMGQLLSAIYTQYRNTRTYLLFDNYLQLDEDLHLFLTLYDLASSNNANFDDWKKVY
                     LSARLANMYLKSALQNLLRLSPEVDLFRNIIETSDLTDLRYLFRYGNYISDNEFALAD
                     FMAQYPSEELDKLAKYIIQCWIDGFIRAKKDYSLKKYVNLVIPCGMERLGKLLIEELK
                     RLNFIPLVTQPYTKGANRQYNYDHRFDIALLLDRSYVDESLKIAEDILEDLKDLIIDQ
                     AGPVYVELFGELPFTPEIKSSTIQLSPEQEQLYRELSSKNSFLYMKYYHPDETSFTII
                     AFPSPEIGPQFPEIFADTVKINTLDSHKYALIQQKIIDVLDNADAVYVKGKPGNETDI
                     IVKLHPLSDSEHQTKFENCVADVNIPVGEVFTSPLLKGTNGTLFVEDIYLQNLRFFNL
                     KIQFEDGWIKDYSCTNFPDPEESKRYIYQNLLLPHQTLPLGEFAIGTNTTAYQMARKY
                     NIISRLPILIIEKMGPHFAIGDTCFSHEEEAPHINFLNGKSMIAVENEKSTTRKEDPI
                     NAYTNKHIDITLPYEMLDSITAIGYDGTRTDIIKNGRFVLPGTEELNIPLDEGW"
     gene            complement(23997..24428)
                     /gene="smpB"
                     /locus_tag="PK015_00580"
     CDS             complement(23997..24428)
                     /gene="smpB"
                     /locus_tag="PK015_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOL32999.1"
                     /translation="MKSIKNRRAEHDYYILQKIEAGIVLTGSEIKSIRAGNVNFRDSY
                     AKIEDGECWLYNLHISPWGKSAHFVPDPNRKRKLLLHKREIIRLKSKVEEQGMTLIPL
                     ELKINEKGLCKVILALAKGKKIYDKRESLRREAIERDRERG"
     gene            complement(24425..25747)
                     /locus_tag="PK015_00585"
     CDS             complement(24425..25747)
                     /locus_tag="PK015_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOL33000.1"
                     /translation="MNKEIDLIVKGGIILCMDEEMRNLENYQIAIDEGKIIDIYPAES
                     KEYIATKTLDASDCIVMPGLINAHTHLPMTYFRGLADDLPLEIWLNHYIWPLEAKILN
                     RNFIYNASLHGAAEMLKNGITQIHDMYFDMPAIADACTKVGLRAIIGEALLDGNTSAP
                     ERFNELGNKMLELKQRYKDNPYVDFNLAPHSIYGCSRQTLEKCAQVAADTGILLHMHL
                     SETKSEVEQCIKEHGLRPVFYLKDIGILDLPAVYAHGVWMDEEEIELLSEVPASIAIC
                     TESNLKLASGILPLAAYRKYRVNLCFATDGVASNNNLDILSEMDVTAKLHKVINNDPA
                     FLPAVDIVRMATCDAAKALGISHKRGSLEIGKDADICILSLSELESQPLYNPYSHIVY
                     ALSSRNVRDVVVNGKIVLESGKLMQVDESALIAIAKQQKEYILQQLEN"
     gene            complement(25740..26783)
                     /gene="mtnA"
                     /locus_tag="PK015_00590"
     CDS             complement(25740..26783)
                     /gene="mtnA"
                     /locus_tag="PK015_00590"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOL33001.1"
                     /translation="MIINQKEYKSVWWEHNRLYAIDQNKLPWELSIVEFTDYLQVAQA
                     IKDMTVRGAPAIGTAAAFAMALAAQVAPEDRFRVSLREARKHLIETRPTAVDLLNGVN
                     YVYEQTIKFIPDIKHCRQVAILAAQEFARHSEEDCRTIGEIGSAIIQDQSRILTHCNA
                     GALATVDWGTALAAIRFAHRQGKKIFVYVSETRPRFQGALLTAFELEQEGIPHSIIPD
                     SACGFYFWKKEIDLVITGADRVCLNGDVANKVGTYEKAVLARYHRVPFYVAAPLSTFD
                     LGCIRGKDIPIEFRSEEEVKQFAGVPTANPSSPALNPAFDITPAKLITGIITPQGIFP
                     ASEAAQKVQKYYE"
     gene            complement(26807..29416)
                     /locus_tag="PK015_00595"
     CDS             complement(26807..29416)
                     /locus_tag="PK015_00595"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOL33002.1"
                     /translation="MAKKIVNPKISPVGKQEKKKMVTQQEVKKKLQEKGKPSSVPVSV
                     IEPGETLSQKKWLPWVLMIFLFVFVSLLYFPVAFQHMVPPASDTAQWEGAAHQIIEYN
                     KTHKDPALWTQSMFSGMPSYMISFPNRYPFLENITRITDKVINWRIFLLFVGALGMFL
                     LLRYLKLDIWACFLGAVAFMFSCHWMGLLEIGHNTKFRAIMYIPWVIWALIHLRKKPG
                     LLGLGFLSTTLIMQLRENHPQITYYLYLFVGLYWIWQLIVSLKAKDLKKFWQFTGLLI
                     LAFLITVLAVMNPYLSTWEYSKYTPRGSSGGLETSYAQQWSFHPLEIVTLIIPNFFGG
                     VSPDYWGYMPFTQTYNYFGIVVLALAIIALCGKKHRKTATFLGISSLLFLIMSFGSAT
                     PHLSNLFLKYLPYFNKFRVPSMILVMVQILAVIMAGLGLDTILSLTEEERKQLSKGLF
                     KAFWICGIIFLLWLILAKPIFGGLPFASAQEKQQLAKYNMTEIPADIRATRLNMLYTS
                     GILSLLLLTVSIGLAYLKTVKKLPQMIFVVLMSVIVFIDLYTYTGKFLKKENLQPVQQ
                     YKDRFAAQDYDEYLLADDSNYRIYPIEQNIISDARLPKTTGEWAYHHQIVTGYSAAKL
                     ARYDALMNYLQDSVKGPGEWRDYLIGIFSPEKGEIPREKPTNIMDMLSVKYMLHPEHL
                     PYDSLLVNIHPVFDGSDNVIIYKNNRALPRAWFVDKVQKVAPADSILPLLREETFNPR
                     NLAYVETDIKDVQAPDSCSVVQTVNELHKLAYDVYTDKPSFLVLSEVYYPAGWKAKLD
                     DKEIPIYPANYVLRGLQIPAGQHKLELVFAPESYTISIRLSLIGLLASLLTLMGGLIW
                     NYNRINKVKEEKV"
     gene            29519..29932
                     /locus_tag="PK015_00600"
     CDS             29519..29932
                     /locus_tag="PK015_00600"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOL33003.1"
                     /translation="MIFEFQKRIYGYECDVYGHLNNANYLQLLEAARVEAMIKMGMSI
                     KRMRELNLQILIRNFNLDYKRAVQHEDLITIRSWFDEISRIKQHWIQQIYDSSGRLCF
                     EAQMLGVFASEGRAQRLPVEVFEVFHLYQESKAPS"
     gene            30085..30795
                     /locus_tag="PK015_00605"
     CDS             30085..30795
                     /locus_tag="PK015_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOL33004.1"
                     /translation="MSSIADNIKRIKEQTVEKLISLGRPEDDITIVAVTKTHPVAVIE
                     EALRNGISHIGENKVQEAIHKIPFITVPYEGFHFIGHLQSNKINNLLKLKPMLIQSID
                     SLYIAQQLNRSLGRKNITQDILIQVNTTAETTKSGVNPENAIDMIWQIAALPCLNIKG
                     LMTIGMLSPNPENTRPYFRKLWKLKEKIEAEKIPEVQMKYISMGMSEDYLIALEEGSN
                     MLRLGTIIFGTRNYGGTN"
     gene            30792..31436
                     /locus_tag="PK015_00610"
     CDS             30792..31436
                     /locus_tag="PK015_00610"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HOL33005.1"
                     /translation="MNYLILILIIIAAYFYGCISSARVVAKTFRSLNIYKVGTGLADT
                     ENIFVNVSRPLGVLVGLLDILKAFFFLLVIEYLVRWLNFYGIVEGADLLYNKNIMLLY
                     GLAMLVGHCLPATNNFKGGRGIFTYSGYLLYFVPAPMIISLIVAGVITFIWKQVRFAQ
                     YVIVILPVLLTHIFYAFIPTFRSNLPPHFIVIIWAIAIIMGVLNFILSKRLGEI"
     gene            31438..32400
                     /locus_tag="PK015_00615"
     CDS             31438..32400
                     /locus_tag="PK015_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOL33006.1"
                     /translation="MSNHASEALIKEKKTSLVDVESKTKTFLSPIDIITIAYCAWIII
                     YMIIGIFVGRAEQPAIHLPRFISIITAVLLMAWLERNYASVMNPKLLHFLQFIRSIYP
                     VLLFTYFYTSLHSVCRILCPEWLDPFFSNIDYKIFGYYPSMVWGQKYDNWFAQEFFHF
                     AYFCYYPMIAGLPTYLYFKNKEGFKELIFNLTFVFYFCYFFYSLVPVVGGRYFPEGYQ
                     LAHSYRYGFFTRLMAFIYNHSGHWGGAFPSSHIAITIVLTIAGLKYVRKWGYVFCVIA
                     FFLSISTVYCHYHWFIDAVAGIVTGIAGYFVANWIRQKLEKEPK"
     gene            32397..33422
                     /locus_tag="PK015_00620"
     CDS             32397..33422
                     /locus_tag="PK015_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33007.1"
                     /translation="MKRLLTLLFLCCSITIFAQNSDMPIIQSYDYALPENNVSPIAIG
                     MGGLNVTYAGDFYSSYSNPALIGDNDFSVLMGAFRIKSDEAVNFAEAAQFSNILKPKQ
                     LKYISLLAKQTAWTYQPVASVHIVEPPETGVGYRYYDYKLDKVQVTLAGSDDSWPPIR
                     FGLNLKYLTGRLVYLVSTNPNSFIDDKLNGFSTDLGFTLQTGSFTYGLAAYDIFSRLY
                     WEHYNSEAIQSRVAMGIQYGTDNFNLNLGLQGKIAQSTDTTYHLGGQYIWTWGSQYYS
                     VESANKALAFRAGLYSHNFYGTQNINYTIGGGYNYNIFRFDFSLNNKGMKLKDSELLI
                     SLGVGMP"
     gene            33426..35390
                     /locus_tag="PK015_00625"
     CDS             33426..35390
                     /locus_tag="PK015_00625"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HOL33008.1"
                     /translation="MGNLTFLNAGFLFFLAATILPLIIWLLAKKKPKQVVFPTIRFIK
                     ASQEQEKKKTRFKNILLLIIRMLIILLVTLAATRPLFSSSQLKPSANHPPTALAILLD
                     TSYSMDYVYDSRSNLDRAKDAIKKINSLSNSDDRLILVTSDENWNRLHSQIYAGSIPE
                     DIIEPISVTYEPLTISLMLEQAKSRLQETQFANKEIYLITDGQKQEYPSPSDITLQVI
                     NIAENEDFSNLSCSQAQVLPQLAEKSRRQTIEFKLTNHGNKDRQEVLVRTVLGEAKVG
                     EKFAHLPARQTVNQTMVIDLQKDGWQSGYVEVVDDHLEQDNRSYFSFPFYLYPQIAVI
                     TQNRSLPPALYSLLRVYAGSEGKINLIEPQQLNLSMLNQYQSYVFLGTPSFPPQMREV
                     INTLQSSNRGALFCLSANLSTEYRAFLENIFKVKFGAWQSQPKSITYVNPHHFTTSLV
                     AGKSLQFNNISDFWHLEGREINALLAGSGETFALAKDNFILWNFDPGSERSTFLLDAA
                     FPVFAYRCLEHTSSSIATGEKYTVGDIINTHSVITPAGENLLFSNRPYKLSQPGIYIL
                     RKADGTETVISVEAPRHESEFIPMDYQELKNVQLLGEDWQDKIFHTRLGYELWKILLA
                     IALALVILEIILVKSEELRSIPEAVPSSGS"
     gene            35393..37255
                     /gene="dxs"
                     /locus_tag="PK015_00630"
     CDS             35393..37255
                     /gene="dxs"
                     /locus_tag="PK015_00630"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOL33009.1"
                     /translation="MILENIKDPSSIRKLNTEEMNLLAKEVRKRIIDVVSKTGGHIAA
                     SLGVVELTIALLNVFDPLKDRIVWDVGHQTYTWKILTGRNDRFETLRSLNGLSGFSNR
                     NESPYDAFTTGHSSTSISAALGMACGRDLNRETGHCIAIIGDGALTGGMSFEALNHAG
                     QLQKDKFLVILNDNAMSISPNVGGLQKYLARMLASRPYNTLKQQIWDLSSTLPANLRR
                     TFILGAQKLEESMMNILVPNIIFEDLGFKYVGPIDGHDINLICTMLRRIQRHMVGPVF
                     LHVVTQKGKGYLPAEKETVLFHGVEPFDAESGTLPANGKKSWSEVFGNTICDIAENNP
                     EVVAITAAMSSGTGLTPFEKRFPKRFFDVGIAEQHSVTFAAGLACKGIKPFVAIYSTF
                     LQRAIDQVIHDVALPKLPVVFCIDRAGLVGEDGPTHHGTFDISLLNSIPNLMIFSPAN
                     DEELIAMINFAATYKEGPIAIRYPRGSAFHSEEVVTEFIPGKAQIVKEGENIALLSEG
                     GAWQIALGTWNLLNSKGLNPWLINLRCLKPLDEDLLKNLGKTCSHIFTFETNVVIGGV
                     GARIGQLLEESPCKVINFGYPDIFVPHGKIEQLNELIGFTPAHLSEEIQRHLSL"
     gene            37256..38644
                     /gene="mnmE"
                     /locus_tag="PK015_00635"
     CDS             37256..38644
                     /gene="mnmE"
                     /locus_tag="PK015_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOL33010.1"
                     /translation="MANLSEPICALITPPGYAAINVVRISGKGSIGIVAQYFKPRKKL
                     LNCPSHRVVFGTFYSREGRAIDQVLCTVFRAPQSYTGEDVVEISSHGNPQIASQILEN
                     LLLDARMANPGEFTLRAVLNGKMDLPQAEAVNDLVFASGAMAESAALMQVQGILSQHL
                     QNLLNDISDTRLRCELAIDFADQDLPPVDPEDLKNRISHILKQAEELYAEGVSQGKYI
                     REGIKISLVGAPNSGKSSIFNAFLKYNRAIVNPHPGTTRDYLEERVNLQGYTLILYDT
                     AGLRISSDEVEQEGIERTRELMQNSDLILYLLPVDQEIDWKEIDELPPEIKNKILWVA
                     SKYDLINPDFSTVKNFDNLNGQSPDSAIPASVYAIEGLEKLQQAILNHFALPQKWVDR
                     PLVTNARHLAALSRAISSLKNALQSIDNQAGYEFTAFDLIAASQALEEILGIVTTEDL
                     LTNIFNNFCIGK"
     gene            39190..40122
                     /locus_tag="PK015_00640"
     CDS             39190..40122
                     /locus_tag="PK015_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HOL33011.1"
                     /translation="MVQYFKVQEQRFVPAMSPNEAFWIHLDNPNDEEIKQLIERFNLE
                     EDYITDLQDADENSRMEYDGGAVLIILRVPLYYRHRSATIPFATAPLGIIAVQDKLIT
                     VSFFATEVLTQYLEGKHKPFNITQQSFLLQIALRTAIYFLKFLKEIIRRSNNIETELY
                     QSMRNKEIIRLLRLEKSLVYFSTSLQSNEIILERMQRSRWLNQDPDAEDMIEDVIIEN
                     RQAIEMTNVHSSILSSMMDAFASIISNNLNIRMKFLTSVSIVLMLPTLVASIWGMNVA
                     LPLQESKYAFLILMGISAVASTLVVLYFIHKNMF"
     gene            40136..40507
                     /gene="queD"
                     /locus_tag="PK015_00645"
     CDS             40136..40507
                     /gene="queD"
                     /locus_tag="PK015_00645"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HOL33012.1"
                     /translation="MYKLSVYDYFSAAHRLRGYEGPCSNVHGHNWKVKVTFQTDTLDS
                     LGMAIDFKVLKAKLWEILNQLDHTFLNELPIFKDINPTSENIAKYIFESISESFKGSS
                     LSLLEVELQESDKVSVIYSNA"
     gene            40500..41111
                     /gene="yihA"
                     /locus_tag="PK015_00650"
     CDS             40500..41111
                     /gene="yihA"
                     /locus_tag="PK015_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOL33013.1"
                     /translation="MLRFVQSYFVKSAVEPKDYPASAYPEFAVAGRSNVGKSTLINLL
                     TGYHQLAKTSSHPGKTRLLNFFLIRWKDDEAQKEGHLQLTDLPGYGYAEVPLAEQEKW
                     RKMINNYFDQREMLKGIIVLVDIRLAADPKDIQLLEMLKLRGIDYCVVATKADKIPKT
                     KLNRTLQHLAKGLGLKDKDIFPVSALKKTGLEPLYNWIGAHLI"
     gene            41114..42961
                     /gene="mnmG"
                     /locus_tag="PK015_00655"
     CDS             41114..42961
                     /gene="mnmG"
                     /locus_tag="PK015_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOL33014.1"
                     /translation="MTRDFDVIVVGGGHSGIEAALASARRGAKTALFVLKLETIGRMS
                     CNPSIGGPAKGHLAKEIDALGGELGYVADLSGIHFRMLNRSKGPAVWAPRAQNDRLLY
                     SLLMRQHLEEQDNLSLIEASVSELLVEKGSIQGVRILQGKEYFAPQVILATGTFLNGL
                     IHMGNISFPGGRSGEPASEALSNSLLKYGLKLGRFKTGTPPRIDMRTLNYNLLEEQPG
                     DPEPMGFSQYREVELSNKVSCYITHTTTETHNIIQQNLQKSSLYGGIIKGIGPRYCPS
                     IEDKIVKFPQRESHHIFIEPEGLDTCEGYVNGLSTSLPPDIQEKLLHSIPGLENAQIM
                     RYAYAIEYDYINPEEILPSLECRKIKGLWLAGQINGTSGYEEAAAQGIMAGINATLAL
                     EGKEPIILSRTQAYIGVLIDDLVTCGTNEPYRMFTSRAEYRLLLRQDNCDERLMPLGY
                     QLGLVSDVRWQKFKHTMELKEREMERLKTEKSINHPDLKEPQKFAVLLKRPDINIEKL
                     KLYGYKPSLEVTPDILRRCELELKYEGYLQRAEEEMQRFRASENIFLSNDIDYYSINA
                     LAWEAREKLMKIRPHNLAQAMRIPGVNYSDTAALLLWLKKQQKIERKAE"
     gene            42958..43695
                     /gene="kdsB"
                     /locus_tag="PK015_00660"
     CDS             42958..43695
                     /gene="kdsB"
                     /locus_tag="PK015_00660"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR00466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOL33015.1"
                     /translation="MKAVAIIPARYDSTRFPGKALAKLLSKPLIQWVWEAVINANLFD
                     QVIVATDSIQIKEAAENFGAIAILTRKEHPSGTDRIAEVAEMLDCDIVVNVQGDEPLI
                     NQKALSPLLHVFENSPIQIASLMTKIKDLKQLSNPNLVKVVVDNASNALYFSRSAIPF
                     NRDNIHFEDYFQHIGVYAYRRQTLMQLVRLPAGKLEQVEKLEQLRALENGIPIRMVTT
                     EYNGMGVDTLEDLLNLEILLKEMNYEV"
     gene            43685..45112
                     /locus_tag="PK015_00665"
     CDS             43685..45112
                     /locus_tag="PK015_00665"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HOL33016.1"
                     /translation="MKFKRICIVILSLTISCIYSTPFSVFYTGDTHGVYEAQLDKERG
                     TLMGGYLVLEEVLNQKRTESKNSIYVDTGDQQTGTIFSSLVEDGIYGGAVIEVFNQLA
                     LDASVFGNHEFDFSYTNTRDLAKKANYPFVCTNLIDKTTRQSVSGIPYAILERNNIKV
                     GIFGITLEKLPEKVKAENVSSVTILPPIEAINKYLEEVDKKTDLIIVLTHQGFEADSL
                     LAVNLDNRVDLIIGGHDHIKAEKPHFINGKYLLYSGSHLNYLGLAELEVEDDKITSLK
                     NQLIPLEIPVKEFDTPLAKYIQEKTVKIEEEMSKVIGFIPEDWIPDKYQSTTLSRWIA
                     SALKTEYERTYHPDMAIINNGGLRKVIPAGPVTLRDLNELLPFNNTVVVFSCYGKDLL
                     TFFQLNELHSQEKPFDICEYTRLDSIESDKIYRIISHDYIAGQWDKYLGFKPFDIYDT
                     GEPFLDAIIHQVQLQFSPINEKDWD"
     gene            45117..45995
                     /locus_tag="PK015_00670"
     CDS             45117..45995
                     /locus_tag="PK015_00670"
                     /inference="COORDINATES: protein motif:HMM:NF014609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HOL33017.1"
                     /translation="METTKNINKKENLTEEEAISIGKYDPLWIINAFSRSFYEGNPAA
                     IVALSDFPSDKKMQKLAKEFNLSETAFFCLRSPDLAQIRWFSPTQEISLCGHATLATA
                     SVIFSHLYPEKDKFSFITSNGMIETEKYNHLIQLTLPLEEVIPFITEEKVLKALGKTK
                     PAESYLAPESHNLILIYDNVKKIKSLKPDFPTLLSFKDLPFTGIAVSALDENGYVCRY
                     FAPWEGINEDPVTGSAQTYLAPFWSKRLNKKLLAVRQISSRGGSMEVKVLSSYLLVRG
                     TTYTCFYGNLNSGWRE"
     gene            46094..46576
                     /locus_tag="PK015_00675"
     CDS             46094..46576
                     /locus_tag="PK015_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HOL33018.1"
                     /translation="MEIIIEELKFPSDANIILGQSHFIKSVEDLYEIMVNSVPGIKFG
                     LAFCEASGPCLIRKEGTDNELMECAIENMHRLGAGHSFLIVMQNAFPINVLNAIKNCP
                     EVVNIFCATANPVQVILAKTEQGNGILGVIDGNSPKGIELDTDITHRKRFLRNLGYKR
                     "
     gene            46577..47128
                     /gene="thpR"
                     /locus_tag="PK015_00680"
     CDS             46577..47128
                     /gene="thpR"
                     /locus_tag="PK015_00680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOL33019.1"
                     /translation="MNYRTFIALEPPKSVFLNLKEKLNTFKSTQGVNWVKEQNLHLTL
                     LFLGDVDSTRINELSGILEQATEIAAPFNLSLQGLELFPYKEPRLVWATLLEQDDSLF
                     QWHKSLLKEVRKAGFEPDIKPLKLHITLGRIKTKLPVSKEREILESKVDKDFFSYDTI
                     TLYRSILKPEGPVYQILDQYNLS"
     gene            47140..48195
                     /gene="recA"
                     /locus_tag="PK015_00685"
     CDS             47140..48195
                     /gene="recA"
                     /locus_tag="PK015_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOL33020.1"
                     /translation="MQDKNKEVALKTAISQLEKKYGVGTLMRLGDQPLQQVDVIPTGA
                     FNLDIALGIGGIPRGRITEIYGPEASGKTTLALHIAAEAQKLGGIVAFIDAEHALDTA
                     YAKNLGVQVEDLLLSQPDGGEQALEICETLVRSSAIDLVIIDSVAALVPKAEIEGDMG
                     DSHVGLQARLMSQALRKLTSIVSKSNTAVVFINQTRMKIGTAAFMNPETTSGGVALKF
                     YASVRMEVRFAGAIKSSGPDAEVIGAKTRVKIVKNKLAPPFKTVEFPIIFGKGISNTD
                     IIMDMAIDLGIIKKSGSWLSYGDLKLGQGIEKTRQYLNENPALLEEISNLVKQNVKPE
                     KMFESPTTDETEDIIQE"
     gene            48167..48775
                     /locus_tag="PK015_00690"
     CDS             48167..48775
                     /locus_tag="PK015_00690"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOL33021.1"
                     /translation="MKLRISSKNKYLATIELEDLPWGILPIKQISSLYPSDYEGEISE
                     SEANQIIKMLEDYAFNLLVKYLAKEEHSQYQCVLYLQRKGFCSEIVQDAIHRCLELNY
                     LNDQRFAELLISSYIKRKASKRAITAKLSANHIPINVWKPLLEELYTEEEKASNLEEL
                     LKKYYSTHSNLPPEKLKERAFGYLFRKGFDPDDIRDAIENLS"
     gene            complement(48791..49891)
                     /locus_tag="PK015_00695"
     CDS             complement(48791..49891)
                     /locus_tag="PK015_00695"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOL33022.1"
                     /translation="MIIEELLQRLNEFAPFSQAYEWDNIGLLVGDSKREVTKVLITLD
                     VTFKAMQKALELGANIILAHHPVIFHSLKHITDPLLLSLVENQINVISMHTNLDVAPQ
                     GTNYALATVLGLENLESLSSEQGEKWFHLSVTVPAGYTENVADAVFKAGGGRVGNYIY
                     CSTRHPIKGTFKPLEGARPFISSKEDDKFTQVAEEELEFMVQESRLFDVVSAIKISHP
                     YETPLLYYFPVTTPQPLFGLGMTGILPEPLTLSETLSWIKDKLGCPNPKLWTAGLPLS
                     TSIQKIAVCGGSGSSLIRLAEQSAELYITGDVGYHNLMEAKIPIVDAGHFYTEYPVLE
                     VLKGQLINWNLESYVLSREECEYWQNTHSYHN"
     gene            50619..51227
                     /locus_tag="PK015_00700"
     CDS             50619..51227
                     /locus_tag="PK015_00700"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOL33023.1"
                     /translation="MKKSIILAVLLLVSIGLSFAQPYFGFRGGLNVTNVKITDDDSDE
                     AKTDDRYGYHGGLFMQYPLGKYFIIEPSLLYSQRGYERTIEIPLFEDIKTKVNMDYIE
                     LPLALKFDINISDVHIQPAVAPRIGYAIVTQYLVNEEEADFPNDPILLDYGVDFGLDI
                     TFLNQFLIGARYSLGLADIYDDDNEDITTTNNGLMISLGVMF"
     gene            complement(51286..52182)
                     /locus_tag="PK015_00705"
     CDS             complement(51286..52182)
                     /locus_tag="PK015_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HOL33024.1"
                     /translation="MTLEQELYQQYIRGEGKSPCTITAYMKDLSQFYNFLERYFEDGN
                     IQLEQISPQMIRDWFLHLHKQQVSNRSLARKAAALQSFFSWLKLTNRISKNPMDKIKR
                     PKFEKGLPHYFTEEEMLILLNLPDQSSIFGVRNRAILELLYSSGLRISELADLELNDI
                     DLKRALVRVKGKGNKERIVPIGKPAIEAIEAYLPVRDKLKQEYSSHKLFLTKSGKDFD
                     HRQLFKILHHYFQIIARQKGYSPHTIRHSFATHLLSRGADLRALQEMLGHSNLETTAI
                     YTHLTLDDIKKAYLKGHPRGKE"
     gene            52261..53016
                     /locus_tag="PK015_00710"
     CDS             52261..53016
                     /locus_tag="PK015_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HOL33025.1"
                     /translation="MNHYIADSARLGKEIKVGGNTIIMDDAVIGNYCQIGNNVVIHPG
                     TYIGDNVRIDDNTVIGKKPLTSPRSIFKTPQELKPAIIGANSQIGANVIIYAQCEIGE
                     SNMIADLATIRENVKIGDLNIIGRNVTIENYVQIGSRNKLETNCYITAYSEIEDYCFV
                     GPCVATSNDNYMARDPERFNHFKGVTMKRGARIGVHATILPGIIIEEDACVAAGSVVT
                     HNVPAGKVVLGSPARILRDVPEAQLLKNNLDKS"
     gene            53013..53729
                     /locus_tag="PK015_00715"
     CDS             53013..53729
                     /locus_tag="PK015_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HOL33026.1"
                     /translation="MKALLIIPTYNEIANIKNIITASLSQSPDLYILVIDDNSPDGTA
                     KAVKEMMENEPHINLIERPKKMGLGSAYVQGFKYALNNDYDYVLEMDADFSHNPADIP
                     RLLEAAKKYDVVIGSRYCQGVNIINWPFRRLLISYFASKYVRIITGMPIKDPTSGFKC
                     FRRRVLEAIDLDSILSDGYAFQIEMNFRAWVKGFTIYEIPIVFTERRDGVSKMSRQIV
                     WEAAWMVWRLQIMKLLGKIK"
     gene            53744..54289
                     /locus_tag="PK015_00720"
     CDS             53744..54289
                     /locus_tag="PK015_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33027.1"
                     /translation="MKKVWITTLLMIALLSLFAQPTIPRVYVQKLVLEDGTNPFITWY
                     KDQTSPEYLFRAWIWERPDEILSTETHTYHHLAITQVGDGKKVPFMVVSRVNLGNFPS
                     QWHAGETLHLELTHKETGQKVEWDVYIPEGTATIMLLDTPQIIPPYKEVKSEKIIPKT
                     DNSSEKPKENNSSCSNCPHCH"
     gene            54302..55318
                     /gene="ruvB"
                     /locus_tag="PK015_00725"
     CDS             54302..55318
                     /gene="ruvB"
                     /locus_tag="PK015_00725"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOL33028.1"
                     /translation="MLERINTPFERPEDKEFDRALRPRTLNEFIGQTHIKQLLDISIK
                     AAKLRKESLDHILLYGPPGLGKTTLAGIIANELGVNITVSSGPVMEKPSDLAGILTNL
                     QRHEVLFIDEIHRLSHIIEEYIYPAMEDFQMEIILDNGPSARTLRISIEPFTLIGATT
                     RAGLLTPPLRDRFGIILRLDYYDLDSINQIIRRSANILGIPAEDEGIAELAHRSRGTP
                     RIANRLLRRVRDFAQIMGNGVITRDIALQALEMLQVDNEGLDEMDKRLLSTIIEYYRG
                     GPVGIKTLATAVGEDPGTIEEIFEPYLVQQGFLERTPQGRKATYKAYQHLGIIPPNPQ
                     TEIF"
     gene            55350..56630
                     /locus_tag="PK015_00730"
     CDS             55350..56630
                     /locus_tag="PK015_00730"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HOL33029.1"
                     /translation="MIPRKGYKANITSNLVNQILHLIIGAATSIIASRVLGPQGMGYV
                     AYAILIFTILGNFGHFGLNNAVIYFKKRKKVNPTHLNNVNVTLLILFAVAISSVLLVL
                     RSNRWFLSDYNYLYILGGIIYIFSNLLFTHYRSWFVADELIRESNRFIITAFFIKSAI
                     IILLWLTRTLNPLTYFYASVLGMLINAIFLGTHTGEGFRPEIDLALLKAEYSYGGIIF
                     LAACCDYLHLRVDQLMIKQMLGTSFLGVYSIAVTLAELMFLLPASINTALLGKLYNTD
                     EKQKFRQVMAQTYKLTFWICCLLALIGIPLSLLIPFFYGKAFAGAIISTQILLIGTIF
                     SSCAQVSMQYFFSTGKPQIHLLTTSCSLLINIALNLWLIPLKGITGAAIASCISYFIY
                     GLFYLIVFIKKEEFSLHELLSISLSDLKLLWSSK"
     gene            56627..58201
                     /locus_tag="PK015_00735"
     CDS             56627..58201
                     /locus_tag="PK015_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HOL33030.1"
                     /translation="MKTDLFNGYFWYQNKLFNDLPPDFFPSEYNPETYTSILKELNGC
                     FRLIVEGNDFLIAAVDRVRSLPLFFYQKGKDLFIDPDAFELKKLLNNPELDNLIFTEF
                     LLTAHCCQNGTLYPEIKQLEAGQFLIWHKSEARLELRDYYIYQHLFEPIENPIEAMDE
                     MHQHIIQRLIDSAHNRTLVIPLSGGYDSRNIAYMLKRADYKNVICFTYGKPYDGDCST
                     SRKVAKFLNFPWIMVENTRPMWYEAFHSEEMLQYFRFSTNLSSSFHVQDWLAVKILKE
                     KELIPSDAIFVPGHSGGALQGQYLPLIFENRDKMTSKELIQTILRQFYHLWEYPMEKL
                     YSLFSDRIGSYLTIPDTVLTEIATSIYDEWAWRQWESKFIVNSVRVYEFFNYEWRIPL
                     WDNEFMEFWQRIPFSQRTHRQLLKQYLQKYQPIPVPAYHDYSFTRRIKNKYARITVGN
                     IMTLGYGRFLDYKDRDAYLNTKIASLLVPELHYPEFINPELPILKAQINAIQALIYIK
                     ELVSGNLDNITLSKQF"
     gene            complement(58184..58714)
                     /locus_tag="PK015_00740"
     CDS             complement(58184..58714)
                     /locus_tag="PK015_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HOL33031.1"
                     /translation="MPVKDWEFKESFMIHLIYGDITKFEGDAIVNAANSSLLGGGGVD
                     GAIHRAAGPKLLEECRTLGGCNPGDAKITKGYNLKARYVIHTVGPIWRGGNSNEAETL
                     ASCYRRSLELARQYGIQSIAFPNISTGIYGFPKEQAARIAIDTVKKDLYENGELEVYF
                     YCFDPENYQLYSELLA"
     gene            complement(58689..59483)
                     /gene="rnc"
                     /locus_tag="PK015_00745"
     CDS             complement(58689..59483)
                     /gene="rnc"
                     /locus_tag="PK015_00745"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOL33032.1"
                     /translation="MKELRKEKGVNKFFQRFIDFINGKRIKELYPKWEKSLKQLQKKI
                     DYNFHDINLLKAALTHKSYLNRNYSESQAPSPFERMEFLGDAILGFGVSRELFNRHPE
                     EQEGKLSKLKSKIVSETYLTLKANALDLGKYLLLSQEEQQAGGAKKPSILSDAMEALI
                     CAIYLDSGISAAMRFIKNNIMNDYESVVNRNELVNYKSILQEYMQSRNQPPPVYITVA
                     EEGPEHNKTFIVEARLKNEVLGKGKGSTKKGAQQEAARDACQRLGI"
     gene            complement(59512..60750)
                     /gene="fabF"
                     /locus_tag="PK015_00750"
     CDS             complement(59512..60750)
                     /gene="fabF"
                     /locus_tag="PK015_00750"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOL33033.1"
                     /translation="MKKRVVITGLGALTPVGNNIEETWSNLVQGVSGVDLIKNFDTSA
                     LPVKIAAQVKNFKPEEHFDPKEVRKMDTYTQYALVASREAIKDAGLMPYTGDNWRIGV
                     ITGVGIGGIYTFEEECIKNHELGPRRISPFFIPKMISNIAAAHIAIENGFQGINFSIT
                     SACASANHALGTAFRAIQYGDADIIISGGAEAAVTAFSVGGFCALRALSTRNDEPTKA
                     SRPFDAERDGFVMSEGAGFLVLEELEHAKARGAKIYAEIVGYAATDDAFHITAPLEDG
                     SGSAEAMKRAIADAGIQPEDIQYVNAHGTSTPLNDKGETLSLKKCFGDYAYKLKISST
                     KSMVGHMLGAAAAIEAIACVKSIETGVIHPTINLENPDPDCDLDYVPNKAQNCNVNVA
                     LSNSLGFGGHNSALIIKKYS"
     gene            complement(60897..61142)
                     /locus_tag="PK015_00755"
     CDS             complement(60897..61142)
                     /locus_tag="PK015_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOL33034.1"
                     /translation="MDSEAIEAKVKQIIMEKLNVEESEIVPSANFIEDLRADSLDTVE
                     LVMAFEDAFQITIPLEDQDKLQTVGQAIDYLKEKLAS"
     gene            complement(61197..61949)
                     /gene="fabG"
                     /locus_tag="PK015_00760"
     CDS             complement(61197..61949)
                     /gene="fabG"
                     /locus_tag="PK015_00760"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOL33035.1"
                     /translation="MVKYDFENMTVVITGSARGIGFAIAEAFAGQGAHPVIIDINQQV
                     VDEAMQKLQDKGYSVSGYVGDVTNSSQMDEVFSAIIEERGKIDCLINNAGITRDNLLL
                     RMREEEWRMVLEINLTGTFICTQKVFKYMMKARSGSIINIASVIGIMGNAGQANYAAS
                     KGGIIAFTKSCAKEFASRNIRVNAVAPGFIETEMTAKLPESVVEGYAKAIPLQKMGKP
                     EDVAKLCLFLASEESAYITGQTIAIDGGLVMH"
     gene            complement(61962..>62438)
                     /locus_tag="PK015_00765"
     CDS             complement(61962..>62438)
                     /locus_tag="PK015_00765"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOL33036.1"
                     /translation="LVKAVNFNTPAQTVISGTESGVAKASELAKAKGAKRVVPLIVGG
                     PFHTPLIHKASLWLAQEMENIPFKDATIPLISNVDALPNTSGQISKEKLAKQVISPVL
                     WVDCVRRMIQDGVGYFIEFGPQKVLSGMIKNIDRDVKVFNMEKIEDWKAIDDAFKG"
     assembly_gap    62439..62538
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(62712..63704)
                     /locus_tag="PK015_00770"
     CDS             complement(62712..63704)
                     /locus_tag="PK015_00770"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOL33037.1"
                     /translation="MPIHNAKFTSFGSYVPKRVMTNYDLEKIVDTSDEWIRTRTGMVE
                     RHYAEPEEAASDLAYIAAQRALEASPLKYRQIEMIIVGTVTGDHAYPSTACILQKKLG
                     MKNIPCFDLSAGCTGFLYSVDVAKNFIENGTKNNILVIGVEVLSKTINFKDRSTCVLF
                     GDGAGATIVSRAERNDISRIIDSVMYADGSQSDLLYQPAGGSRMPASPETVEKNLHTV
                     YMEGNRIFKNAIRSMYAATDELLRRNKLEAKDIDWIVPHQANIRIIESLADKLHFPMN
                     KVIVNIEKYANTSSATIPIAIDEAIKDKKIRRGDLVILTSFGAGLTWGAILARF"
     gene            complement(63710..64708)
                     /gene="plsX"
                     /locus_tag="PK015_00775"
     CDS             complement(63710..64708)
                     /gene="plsX"
                     /locus_tag="PK015_00775"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOL33038.1"
                     /translation="MRIAVDAFGTDKAPFPEVEGSIHAIKEDLCNEIILVGDEHILQA
                     ELDKYYYDHNRIRIYHASERIEMHDHAAEAVRMKKDSSMVKTVELHKNGEADAALSAG
                     NSGAFMAASLLAYGRIKGVSRPAIAAALPTTIEQPAILLDMGANVDCDAENLLQFAQL
                     GSIYFTHCYHKPNPRVGLLNIGEESTKGNALTREAYELLSAAKDLNFIGNIEGKEILS
                     GKFEVIVCDGFVGNVVLKTIEGVVRAVFDMLKEQLEKDWIAKIGAILSFPAYSFLKRK
                     LDHTEFGGALLLGLNGTSIVCHGSSDAKAIKNAIRFAVRTVQSGFVQHSKDYFRER"
     gene            complement(64710..64889)
                     /gene="rpmF"
                     /locus_tag="PK015_00780"
     CDS             complement(64710..64889)
                     /gene="rpmF"
                     /locus_tag="PK015_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOL33039.1"
                     /translation="MAVPKKKTSKARRDKRRTHKVLTEPTVVTCAKCGEPVRPHHLCE
                     KCGTYNGRQIKAIKE"
     gene            complement(65248..66576)
                     /locus_tag="PK015_00785"
     CDS             complement(65248..66576)
                     /locus_tag="PK015_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOL33040.1"
                     /translation="MRQKKFLISRTYRVSIKEGIFAQIYGNLANIGSSFITKFMVLLG
                     ATPMQFSILSSLGQVSAVFQPLGVALTHRLKKRKKVCIWVTGIGRFLSFFIGAAWFFS
                     DRRMGIIYLLVLLFVSAGLQAIGGNIWIAWVSDLIPQTIRGRFFARRNQILAFAGLIA
                     GYFFGYCTDLFEKNGGLLARFFPNPVVIFNPQNQPFFLILVFIIATILGIISLFILNK
                     QPDRKYRVQSDWALREKFSEAFRNKNFRSLLFFGIWWMLATGVGSAFWGPFMLKKLQM
                     SMIQLQTYNTLSIISSLLAYSFWGKFIDRYGNKTAMSICVALGGFNPMLWLFLTPQNF
                     SLLWFEALSSGFMWSGNAIATTNFSLAIAGKGKEQTYSAIYGALGGIAMMCSTLLTGV
                     FFPAPQKLGNRWLEPEQVVFGIGGILRWLSIIPLLFVREKVYSGMKKKYN"
     gene            complement(67059..68270)
                     /gene="hydF"
                     /locus_tag="PK015_00790"
     CDS             complement(67059..68270)
                     /gene="hydF"
                     /locus_tag="PK015_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HOL33041.1"
                     /translation="MSSTPRGERLIITLLGNRNSGKSSLINSLTNQEIAIVSEVPGTT
                     TDPVAKRYELLPLGPVTFYDTAGLDDMGELGEKRIRAAYKVLFKTDMAIFVNDGSPFQ
                     ENELKALQRLKEMKLPVLVIFNKSDLRKPHQNNIEFCQDNDLAWISVSATTKENITVA
                     KEKIIELAPFYLKEERPLVRDIVHSGDRVILVTPIDSSAPKGRLILPQVQVLRDLLDS
                     GAVTTVVREFELNKALKALKSEPDLVITDSQVIQRVVNQLPPQVPLTTFSILFARYKG
                     DLEALIKGCQKIDELKDGDRILIAEACSHHPQKDDIGRTKIPNWLKNYTQKDLNFDIA
                     AGSDFPDNLEDYALIIHCGACMLNQKEMNNRIREAERRGVPITNYGLAISKLHGYFER
                     TIAPLKIPFRA"
     gene            68528..70306
                     /locus_tag="PK015_00795"
     CDS             68528..70306
                     /locus_tag="PK015_00795"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HOL33042.1"
                     /translation="MPGISLIKSRELTNSHLDSIKEFQSSLKQILPHISCEILFHNDD
                     TIIHQTTYRNYEFYYQQSGNWLITFEGRKDDVDFGSMVESLNLFYSRDISTKAMQEWR
                     DKTFKLCKTDFFFLACNLETGTVVFANDDLARMPVYFFHQKGTFIISREYPLVRNLNK
                     ELIPDRLLLALYLLFKYIPGQGTPYEEIRTLDNSSIGVYYPASDSLTLISDISRRMPL
                     ESFNGDRKQRLNDLKDSFVSATDECLGDRRGLLAFSGGHDSRCVAAALKLTQKPFISA
                     TFVNDDISSRNDMKCAMQLAEILNMEHKVLNLAPEDETHYRSLYTLKNGLNFMSVVFM
                     LNFLQKLRKEFDEDYVFFTGDGGDRIMQPLYPSKLKLKEDNILPYLFAKQALFDKEYV
                     AHLFDINSADIDNYLINLLHSYPVSDWTNKYLFFILAENAGKWSFEGEDRNRYFYPSK
                     TPFYNLDFYKLAISIPLSWKDNGVFYWQFMETLNPDILKVPIAGNKLSPDTWNYLLLK
                     RIKNFLSNFSFLINLKKFIAKPQPTSVSTINLVQQRIEILNQHKNIKEFIPNFNINEF
                     YNKNKEFNRAHFTNLYTVLTMITDQC"
     gene            70411..72402
                     /locus_tag="PK015_00800"
     CDS             70411..72402
                     /locus_tag="PK015_00800"
                     /inference="COORDINATES: protein motif:HMM:NF019295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOL33043.1"
                     /translation="MQKIGLIFFFIVLFSFLEAEIIQVGNGTLTNQSLPIEAFRSYSY
                     SQQVYPATLINRAGNISSISFQYNIVSNHFLLNNEEWKVWLGHTSRQTLSTFVPIDSL
                     ILVYDGVLTNTNFSGGLPGEGWLQINFTTPFYYNNEENLLIAVDENSPGGSSTSDEFL
                     CTAAPVVRGIVYTDININPDPANPPVPPIENFFFARNAYPNVKLEFTSTGYLPTQPVP
                     ANGATGISINTGLSWQSSASSFDLYLGTSIDNLTLLAQNLNTTEWYPYEPFQLWHTYY
                     WQVIAHQGENAYSGPIWSFTTAGLGIGAPQNLRGYYISGRVELNWEPPQNGNPILYRI
                     NRNGNFFAVSYNLSYQDTEVCVGQTYYYNVRAENAIGEISDPSNTVTVHIPENIPHLI
                     LEESFEECSPFSGIIAQWQNLDIDLSETWNWQEIDFPGEGQAHSWISFFPLLTTPPLT
                     IIQPHSGSAMLASLASVNPPNNDWLISPQFFLGNNPQLKFWARSHTSDYGLERLKVLI
                     SNTDTNLSHFQPLHTGNWISVPAEWTEYTLDLRTWENQFVYLAFNCVSWDALALYLDD
                     IIITGEGGYVAINDEVISSSHFLVYPNPARGNFTVSSSDKVLFNIEIYDIRGRKLLSV
                     KQVTEFEKNKYLPELAAGIYLINLESAKGKFQRKVVILP"
     gene            72411..74936
                     /locus_tag="PK015_00805"
     CDS             72411..74936
                     /locus_tag="PK015_00805"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOL33044.1"
                     /translation="MLSFDIEAFIYSLLTDEKFRNQIVAQQELEARDAIWADFPSQSN
                     PYLLSMLSKRGITKLYAHQKEAIETALKGENVVLTTGVASGKSLCYQFPILQTLLTSP
                     KSRALLLFPTKALAQDQKQKVQELILGLLKENPHIPPIYTGIYDGDTEVGVRRNIRNK
                     SHLIFSNPDMLHLAILPNHSLWSNFFANLKWIVLDEVHIYRGVFGSHFANVIRRLKRI
                     CEFYGSSPQFICTSATVANAKELTETLLEEPVHLVDKDSSPHSKRVFFIINPPIVDYA
                     LGIRRSALMESTILAKNWLQHKGQAILFCGSRRNVEIIYLYLTKDESYKEKIRSYRSG
                     YLAEHRREIEKDLREGKIKLVISTNALELGIDIGGLDAVFMNTYPGTIAATRQQAGRA
                     GRKNNTALAILIAGANPLDQYICQHPEYLFENNPENALISPDHSEILQSQLLCAIQDL
                     ALMENEGFGSLGSEHIFPYLQVLLQEGKIRKANDRYICAPETYPAAEVSLRNLSSQVE
                     IISNNEVIGTVDSSSAMWMVHPQAIYLDKGDTWQVTNLDLEHNKAEVKPVQVNYFTQA
                     TRQTFIECKNLLKKENCGGSDKYFGQVTVTTTITGFKKLRFFNQEVLEWVELDLPPTK
                     LETIAWWMGISPQIVDAVKEQGLWNSEPNDYGKNWQEITKAIKKRDGYRCQHCGALEK
                     DIPHHIHHIIPFRKFDNPEEANNPSNLVTLCPKCHRLAELTVQIQNGITATGYLLVNL
                     APFFLMCDRQDLDVFCEDKSVLTQNNPIILIYDNISGGIGLSRKLYDLHTQLLEAALD
                     LVSQCPCKEGCPSCVGPVAENGVGAKAYSIALLQELIKNLPDR"
     gene            complement(74983..76350)
                     /locus_tag="PK015_00810"
     CDS             complement(74983..76350)
                     /locus_tag="PK015_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOL33045.1"
                     /translation="MKNMRQIAVDQLVKYLTSDPVKKISKIVSLVQKLDRKHKYSDVM
                     DIIADALMGESDVWKKFTEDLFKEVDTKTLQKLAECFLVNVAFVGGPKRDKIKEKYNC
                     NVPLALLIDPTSACNLNCTGCWAAQYGKQHNLSYDILDSLCQQGRELGVYWFLFSGGE
                     PLIRKTDIIRLCEKYQDCYFFAFTNGTLVDEQLCEDIRRVGNFTLAFSIEGNEANTDM
                     RRGEGCYKRVVEAMDMMKKHKLFFGYSTCYTHYNTESVASDEFVDEMIARGCRFAWNF
                     TYIPIGKDAQLDLIATPEQRAYMYKRVQEIRTTKPLIALDFWNDGETAHGCIAGGRSY
                     LHINAAGDVEPCAFIHYANVNIHNVSLLEALQAPLFQEYKKHQPFNSNPLIPCPLLDN
                     PEILIEMVNNSNAYSTQLDGPEPVEELCSKTVLAAQRWAPVAEKLWEERQQIKKEKEA
                     KAAKR"
     gene            76962..77345
                     /gene="speD"
                     /locus_tag="PK015_00815"
     CDS             76962..77345
                     /gene="speD"
                     /locus_tag="PK015_00815"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:TIGR03330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HOL33046.1"
                     /translation="MQALGRQILAEFYECDENILRDEEAIRKAMLEACEIGKATVVSD
                     TFHSFSPYGVSGVVVIAESHVAVHTWPEYGYAAVDIFTCGQTISPWDLFKHLQKCFKS
                     KHTSNMELRRGLFDTGNEPIKHKPY"
     gene            77352..78203
                     /gene="speE"
                     /locus_tag="PK015_00820"
     CDS             77352..78203
                     /gene="speE"
                     /locus_tag="PK015_00820"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002010.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HOL33047.1"
                     /translation="MSYWHIDYHSKYQALTFEVTEVLKSIQSPYQKIEVVETPAYGKV
                     LLLDDDLMLTEKDEFAYHEMLVHPTMFTHSYPQKILIIGGGDCGTLTRVLQHKTVEKV
                     VIVELDEMVTQVSQEYFPQLVSSTQDPRVELIFTDGIKYVHNCTDKFDVILVDSTDPI
                     GPAEGLFRLSFFQDCHKILEEDGILCLQSESPWIPELAELISYVNQDLKSLFPVVKAY
                     GAAIQTYQAGFWLFQLASKKYNPLSKEIENKIKEADLKTKYYNPNIHYGAFKLPTFVN
                     ELLHEPS"
     gene            78370..80931
                     /gene="clpB"
                     /locus_tag="PK015_00825"
     CDS             78370..80931
                     /gene="clpB"
                     /locus_tag="PK015_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOL33048.1"
                     /translation="MNAQRFTIKSQDALQGMQQIAEENGHQEIKDLHLLAAMLKETES
                     LVIPVLQKLEVNTTDLERKVEDALKKLPKVSGGQIYLSQEVLDILHQAEREADQLQDD
                     YISLEHILLALLEKGKEAARLLQNAGVTPDKLLLALKELRGNQRVTDQNPEAKYQALE
                     KYARNLTRLARQDKLDPVIGRDEEIRRTIQILSRRRKNNPILIGEPGVGKTAIVEGLA
                     KRIVNGDVPENLKDKEILELDMAALLAGAKFRGEFEERLKAVLSEVEKSEGRIILFID
                     EIHTVVGAGAAEGAVDASNMLKPALARGTLHCIGATTIDEYRKHIEKDPALERRFQPV
                     MVQEPSVEDTISILRGIREKYEIHHGVQITDTALVAAAVLSDRYISDRFLPDKAIDLI
                     DEACAKLRMEMDSMPSELDEVERKLRQLEIEKYSLAKEEDKLSKQRLEKINEEIAELT
                     EKRNTLRLRWENEKKLFERTRALRAEIESLKLQADKAEREGDYETTAKLRYGIIKEKE
                     KELAELINQRENASGDYEPLIKEKVDEELIAEIVSKWTNIPVSKLIQSEMEKLLHLEE
                     ILSQRVVGQKEGITALANAIRRSRSGLSDTNRPIGSFLFLGPTGVGKTELAKALAAYL
                     FDTEKAMIRLDMSEFMEKHSVSRLIGAPPGYVGYEEGGYLTEGVRRRPYSVILFDEIE
                     KAHPDVFNILLQILDDGRLTDGKGRTVDFRHTIIIMTSNIASQEIYEAEDLEALRPHL
                     MSILQQYFRPEFLNRLDDIIIFHRLSREHIRDIVNIQLNQLAERIKEKGIELSWTDAL
                     VDYLATAGYDPQYGARPLRRLIQKEVEDLMAKAIIAGQVGPQIKLDYQNGSVVVS"
     gene            complement(80997..81803)
                     /locus_tag="PK015_00830"
     CDS             complement(80997..81803)
                     /locus_tag="PK015_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HOL33049.1"
                     /translation="MYKSVKQTNLETRILKMLEGKPLQAAQMLFNDPELQTMQEYANI
                     VAIRRLGYNDHGPVHMRKATLNAIKMFKLLNEAGIKMNLEKEGVGKAEDSLTAVLLSS
                     LLHDLGMSIARDSHEILSIQLAIPFIDRILNEIYNPEELMTKTAIKATAIEGIFGHMA
                     TKHIHSLEAGLVLIGDGSDMEKGRARIPAMLSSEPRVGDIHRTSASAIQRVRIHKGEE
                     KPIAITVEMNASVGFFQVEEVFFPKINNSPVKPYIELYAGVIDRELLRYL"
     gene            complement(81814..82266)
                     /locus_tag="PK015_00835"
     CDS             complement(81814..82266)
                     /locus_tag="PK015_00835"
                     /inference="COORDINATES: protein motif:HMM:NF018448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HOL33050.1"
                     /translation="MRPFDHLTLMQFCWAALSILGFSIRVNLKGWKILLIAIGGGLTW
                     AFYLIFLYFSKSLLFSVFCSVFLVCTYSEIMARLSKIPVSVFVTCVIIPIVPGSSLYY
                     AMQAYIAGDSVVASQNLYKVLLISGTIALAIAVVSSITNLIFSMKNRF"
     gene            complement(82269..83060)
                     /locus_tag="PK015_00840"
     CDS             complement(82269..83060)
                     /locus_tag="PK015_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HOL33051.1"
                     /translation="MNRITSTEALEISMEIGRILLQNGSTANRVELMMCKVCSIFGYP
                     ETEAYVTPTGIFLTVSDGKEIFTTSIKRIENRQTDLGKITKISHLVYDMERQYCKEGV
                     CYWNADKFREELKHIENEKAWPDWFKILCGGLTSGCFCLLFGGSWLEFAVAYAVGVLV
                     SLSLKLLGNLKLNNFLLHILGAALIVVFAKVLDIWVPYIKLDNIIIGGIMLLVPGLSF
                     VNAIRDTMSGDLVSGTARGVEAIFLAIAIAAGSGIMLKLFELWGM"
     gene            complement(83057..84667)
                     /locus_tag="PK015_00845"
     CDS             complement(83057..84667)
                     /locus_tag="PK015_00845"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33052.1"
                     /translation="MNFMLTQMEEVLGVPQILDMIEAEGEFLKSVKKDNLTGYSEDLY
                     DNYYLPIHYKYLEQTMEQLSADKSKLKTLLELLAAIPNLEFPIHKNIKILTVHQLYEI
                     KEYVYYYNQLYYFLEKQKWLDLFDNLKDLTEFFEILDPEGFGLPIFVISSAYSDEIGD
                     ILNKQLKLLQELKESYNQLLERAKKELQIPYLKDSFTVSRSETELADRIIHSPYFVLT
                     AENVANYSFALADDEHCLELKKKISNLQEKLEAEERYVLQELTDIYREWESDIFLSWL
                     SLRRGAWKILLSNFAFKYNCCIPVVWRKKAIIIKKAINLPLKQHLESMGRKYQPIDYE
                     FSGSVSLLTGPNMGGKTTVLKTIGQFCTLLHYGIPLPCESAELPIFKHIWYNQSETDN
                     NADLSSFGREVVSFTQAIQQSGTNLFLLDEFARGTNPTEGELLVSAVLQYLATTKNMS
                     IAATHFTAPTMLPKVTQYSIAGLDLDKIKAQNPKDANERLKILNEAMDYSIKRLKKNE
                     APPLNAINVARILGMPEEVLKYTEIKEK"
     gene            complement(84718..86973)
                     /locus_tag="PK015_00850"
     CDS             complement(84718..86973)
                     /locus_tag="PK015_00850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HOL33053.1"
                     /translation="MLQHEKPAPLFLPVLLWCVGIALSRAIAIPFIWQAIIALVIFIL
                     AIFLKSARIYLILGICLLLGGMRWTIAQQPSVLEEVFKTKSHIQQDSRFLVTNLISSK
                     TNLYKIRLDEVAGIRVREKLLLYSDYKLTPGQEYSALLEIIPAQSDDVLDMYSSLPRA
                     YIRQSLKLEKTTKSIFPIALWRTKLLANLENKLGDEAEFAKALLFSDTSAKGKYRDDL
                     TRSGMIHLIVVSGLHIWFIYAVCMIFLRAFFPPKIAETVFLILIIFYAALNYWAPPVL
                     RAILMIGLMIIARWRNIPLGGAQLLALSLLIITAINPEQLFDIGLQLSFLCVGVIILG
                     LPHIEWIKEKDLPASNIRNKMNGFFDLLLMNLVVGLSVMPLTLFYFGTASLNGIIGNI
                     LGVPLSGVILILSFLILMIPAGNIISASLINSYRFFLCIFNRWTEFVASMPFYLQNRW
                     ITGLQLAGCILLVIGGLSMLKKLEIRWQKLVICLFGIALIIIPQFHHSADRGIYLFNC
                     GTGDCILVSLNEGQKIIIDTGPHYRTSDQSWASQKLLPWLNHKNIKEIDWLILTHLDS
                     DHSGGFPDIISSLKVKNIAVTDETMSDERWSELEKSVLLNKATIHCIEDTISYRIGEA
                     KLKFLHPAKYYYPATSNSSSLIVRLDYQGKRYLFTGDADINAEYYMLEHYPEELKADY
                     LKAAHHGSKSSSCREFIRAVLPEEVWISVSRRNQWNFPHPEPMHNFQLYAQRILSTAD
                     GTIYHPFTQKD"
BASE COUNT        27549 a        16569 c        15983 g        27171 t
ORIGIN      
        1 ttaaatgcat tagattatat atcaaatgct caaacttttt aaatagatag gagaaccaat
       61 gtcatatgtc taatatagaa gtaattatcc ttattctttg ttctctactc ttcatcatcg
      121 gaattattct aattattctt ctttcaagaa aaaatcttcc aatggaacaa ttaaaggatt
      181 ctctgaatca tatatccaca ttgatagaat cttttgaaag gactattaga gatgatatcc
      241 agcgtttgcg agctgattta ttatcttttc aatccaatac ccgaacagaa ttactaacca
      301 cattaaatca gcaaaatgag gctatcagca aagaaaatag aagcaataga gaagaaatct
      361 catcttccct ggcaaagctc aatcaacaaa tgaatgagga tgcagctaag aatcggaatg
      421 aattgggtaa ttctttgcga gatttatccg aatctttatc tcgtagactt caggaacttg
      481 taagtactga acagactcaa tttgattcct taaaaactgc tatggaagca aaaatggaac
      541 agattcgcag tagtaatgaa aataaactgg aagaaatgcg aaaaacagta gatgaaaagt
      601 tacaggatac cctggaaaag agattaagtg cttcatttca acaggtaagt gaaagattgg
      661 aacaggtcta taaaggttta ggtgagatgc aaaatctcgc taatggagtt ggtgatttga
      721 aaaaagtgct ttccaatgtt aaaatcagag gaatgcaagg agaatatcag ctggaagcaa
      781 tattagaaca gatactaagt cctggtcaat atgttaagaa ctggaagcca gataagaata
      841 aaaatgaggt ggtagaattt gctatttgca tacctggtaa agaagatccg aaagaaaaag
      901 tttatctacc catagatgct aaattccctc tgtcagatta taatgccctg gttgatgcct
      961 gggataaagg tgacttagct gctgtagaaa atgcaagagc tgctcttaga gataggattt
     1021 tgaagtgcgc aaaagatatc cagaaaaaat atattgcccc acctttgact actgattttg
     1081 ctttgctttt tcttcccata gaaggtctat atgcagaagt tttacacgat cccactcttt
     1141 ttgatgaatt gcagaaatat catattgtag cagttggacc aaccaccata tctgctattt
     1201 taaacagtct gcaattagga ttccgcactt tagctattca gcaacgttcc aatgaagtat
     1261 ggaaaattct cggtgctata aagactgatt ttgccaaatt tggagattta ctggataaaa
     1321 ctcagaagaa gttgcaagag gcatccaata ccatagaaga tgccagttct cgcagtcgtc
     1381 aaattcagaa aaagcttaaa agcgtggaag aacttccact ggaggaaagt tcagtgatct
     1441 tagaattggg agaggaaaaa gattatcagg tgcaagttga agaaaatacg gatgaggatt
     1501 tatcttgaca gtctctatta aatttaaaaa taaagtatta cttaaaacat aaataaaaaa
     1561 ggaggttaaa atgtttctaa caggacaaca gatgaaagag gtgtttctca aagcaaaaaa
     1621 acagcgtttt ggcattattg cttccaatgt ggtatttgat atgcaagtgc gagcattagt
     1681 ccaaggttat aatgcagtta aatcggatgg actcttacaa atgagcagtg gtgcttgtaa
     1741 atatgccgct ggagaaactc aggatatcaa agctggagct cgtttaatat ccgctatagc
     1801 caaggttttc actgaacaat ttccgcattg tggagtaggt ttacatatag accatgccac
     1861 acccaactat tttgacttta tcgtctattg cattgaaaac aatttggtca gttcggtaat
     1921 gatagatgca tccaaagaaa gtttggaaga aaatatcaga attactaaag aagtggtaga
     1981 tattgcacat cgccatggaa ttatagttga aggtgagatt ggttatatta aaggttcaga
     2041 agacgagata gtttcagaaa cggagcttta tactaaacct gaagaagcac tggaatttgt
     2101 gaaaaagact aatgtagatt tatttgccgc atctgtaggt accaatcatg gtgtcactaa
     2161 aggagaaaat gtagttctgc gtttggattt aattaaagag attgatgatt tgctaattgc
     2221 taatggagtt gaaagaggtc tggttttaca tggtgcttcc ggtttgactg atgaacaaca
     2281 acaaacagct atagccaatg gtgtagtcaa acttaacaaa gatacccatt atcagatgga
     2341 tatggcagca gcagtgcaag cctattggga gaaagaaaaa gatgccattg tctgtcctcc
     2401 tgatgttcca cccgatgtct atatgccaga taaaagtaga tttgatcctc ggaaatggct
     2461 tattaaagca gaacaaagga tgcaaaatac cgtccagagt ttttgcttga tgtctggaag
     2521 tgctaataat agtatattat tataaaaagg agaaacagaa tatgaaaaag gtagctatta
     2581 acggatttgg acgcatcgga cgcttagtgt tccgtgccat aataaaatac catcctgaaa
     2641 tggagattgt tgccatcaat gatataactg atgccaagac tcttgcctat cttttaaaat
     2701 acgatagtgt gcataaaatc tttgatgcta atataagcaa tacagaagat agcatcatag
     2761 tcaatggaaa aactgtaagg gtttttgctg aaaaagatcc ccaaaatctt ccttggaaag
     2821 atctgggagt agattttgtg gttgaatcca caggtgtgtt taataccaga gaaagtgctg
     2881 ctaaacattt aactgctggt gcttccaaag tgattcttac tgctccagct aaagatacgg
     2941 ttgatgcaac tatcgttatg ggagttaatc ataatatctt aactgctgaa cataaggtga
     3001 tttccaatgc ttcttgcacc acgaattgcc ttgcacctat agctaaggtc ttacaagata
     3061 actttggcat tataaacgga ttgatgacca cggtgcatgc ttataccaac gatcaacgta
     3121 ttctggattt agcccacaaa gatctgcgtc gtgctcgtgc tgcttctctt aatatgattc
     3181 caacctccac tggagcagca aaagcaattg gcttagttat tcccgaattg gctggaaaac
     3241 ttgatggtat agccatccgg gtaccagttc cagatggttc tttagttgat cttagtgcca
     3301 atctggaaag agaggtatcc agagaagaga taaatgcagc aatgaaagaa gcttcggaaa
     3361 cctatttaaa gggttatctg cagtattctg aagatcctat agtttccagt gatatagttg
     3421 gtaatagcag ttcttctatc tttgatgcca cttctacctt tgtaaaaggt aaattagtaa
     3481 aggtatttag ttggtatgat aatgaatggg gttattcctg cagagtagcc gacctaatgg
     3541 aatatctctc tcgtttataa agaaataatt gagggcggta atttttttct accgcccttt
     3601 ctctaaatat ggctcgctat ttttatacgg acaaagggaa aactctcggt cctgtaaatc
     3661 gtgaacagat attaaacctc attctaaagg atgtgctaag cctggattcc ttggtaatgg
     3721 acacacgttc tccacgctgg attaaaatac gtgatatccc tgaattaatg cacttccttt
     3781 atgaatctga catccagata tccgattggg ctgaggaaat tgctctttcc ggcgaaagtg
     3841 atgaatctat tcccctattc tttcatatcc ccacagctcg cttaataacc ttaagtattg
     3901 tcacctttgg ctggtatgaa ctctactggc tgtatatgaa ctggcgtttc ttacattact
     3961 tccgtaaaaa tagtagtgcc aaagccttct ggctggattt aatcaatcca ctcggtgtca
     4021 ttagaatctt tcagcacata agcaatgata aagaattacg ggttcaaaaa gaagagcgag
     4081 atttcactgt taatggaata atctggattt tagccctgct aactttattt atctttgcca
     4141 gcggaattat taactatctg acccgtatcc ctttcttagt tagtattttt gtctctttct
     4201 ttgccttaac tttgtctctt tgctgtatta tgcccataca aaattatatt aatactgcca
     4261 atatgcggtt taataagaaa ttatctgctc atacttttgg tcattatggg acaattattc
     4321 tgggtatttt aacctggatt tatgtgctaa tcctctggat acctgcatta tcttagaatt
     4381 attttaaatc cttttcttct ccgctttcct caagatttat agtaggtgga gattgctgat
     4441 tttctggtcg ttgattatgt tcaatggctt cctgaagatg agagcgatgt cttatcttaa
     4501 ttaaccagtt aataaagaac gccaaaagca gaactgccag tgcaattatg acccaaacta
     4561 tccagcttat tccttttttc atttccacgg tcattaattc tgacttattc cacatttgtt
     4621 ccgtgtcaaa ttcccagcta accgttttat tcttataatc tattttatca gcagtagtac
     4681 tggcagagat tattttccag ggtagatgaa ttgtataatt ccatttagga taatctttct
     4741 gttgcttttt atagatgctt gccagaatat catcagggtc agaaagtaaa ttgatatcaa
     4801 tagaatcact ttccgttcca ggttccagta tttcagaacc acctaaagaa attctacgtt
     4861 tatagacgag atgtccttca ctatttttat ttatagatat ctgtcccaaa aaatctacag
     4921 gagtgggttc ttcgcttatt ctattgaagg attctatatt atcaaacttg aaagttacct
     4981 tataaaccat aaaaggtcca tttttctgca cctgggaact aataagatga attcccggta
     5041 attcagattt tctcattatc tcctgtgtat tagcatagtt ggaacgatgg actaaacgca
     5101 aagagacttt tcctgaacca tcaggattta gccacatttc ttcattgtac tccacacagc
     5161 caactaacag gaataacatt atgattaaga gaggtaacat ttttttcttc aatttagaat
     5221 cctcattaca attagcttat cttttcctac taaaagaata atcgattttt tggcaaggaa
     5281 aatatttttg acactgaatt tctaatctaa acaaaaatct tttaaaaaag agtttctttt
     5341 tcggcagata gaattattat aattatagtc agtgttattt tatgtaaatc ctgaaaagtg
     5401 gattttgtaa cttcaggaat acaataaaat taaatatgtg tcataaagtg aaatttatat
     5461 tgacaaaaaa ggcaaaagaa tcgcataaga aaataaatat attgataagg agttgaaaaa
     5521 tgattgaatt acgtcagctt tatcattgca ctgtttgtgg caatgttgta gaagttacct
     5581 atgttggagg aggagaatta gtctgctgtg gtaaaccaat ggaattactg gtaggtaata
     5641 ctgtagatgc tgcaacggaa aagcatgttc ccgtcttgat tgatttgggt gataaggtaa
     5701 aagtaaccgt aggttcagta cctcatccta tggaagagaa acattatatc acaatgattg
     5761 aagttcttac cgaaaagaag gtcttacgtc gtgaactcaa accaggagat gctcctgaag
     5821 caaccttttg tgtgaaagct gaaaaggtta taggtgttcg tgaatactgt aatatccacg
     5881 gattatggaa agcttaatca ttaaaaatag aaggaggaat aaaaatggca tttaaagata
     5941 gtcgtacagc ggataatcta atgaaatcat ttgctggaga agcacaagct cgtttgcgtt
     6001 atcaatatgc agcacgggtt gcagaaaagg aaggatttat tcagattaga aatatcttca
     6061 atgaaactgc agataatgag aaagaacatg ccaaactctt tatcaaacat ctaatagcta
     6121 atggattgga aggagaagcc agaaatatta tggcatctta tcctataggt tggtctcccg
     6181 atagcactct taaaaatctt ctctatgcgg caaatggaga aaaggaagaa tggagtgagc
     6241 tctatcctct atttgctgat atcgctgaag aagaaggtta tacagatata gctaatacct
     6301 ggcgccaggt gatgaaagtg gaacaacagc atgagaaacg ctatcgtaaa ttatatgaga
     6361 atgtgaaaaa ccaaatggtg ttcaaaagaa acgagaaggt ctactggaaa tgcaataact
     6421 gtggctatat acatgaaggc acagaagctc cagcaacttg tccctgttgt cagcatccac
     6481 aagcctattt tgaagtcctt tgtgaaaatt actgagggag gatactatgc aaaaatatca
     6541 atgcatagcc tgcggttata tctatgatcc tgccgaaaat gatgatattc catttgagca
     6601 acttccagat gactggactt gcccggaatg tggagtcggc aaagatatgt ttgaaccgat
     6661 agactaataa acactaacca agaggaaagc ctgcttgatg caggctttcc tatataagga
     6721 aaaaaatgac tgctcaagaa tttcaagaaa taattgattt tgctatcgct cgtgaagagg
     6781 aatctattaa attttatcaa gacctccaga aagaaactaa attccagggt cagatagaaa
     6841 tgctaaaaga attggaagca atggaaaaag ctcataaatc tactcttcaa aacattcgct
     6901 atactaacga ttttaatata gataaagtgc ccaacctaca tatatctgaa tatctaactg
     6961 acgatataga aaagcttgac ctcagctatc caaatttgct ggttatggct atgaaacgcg
     7021 aagaaatttc attgcgtctt tactctgaac tcagcgttag attcgctgat gaacaaatag
     7081 gtaacctctt ccgtaaactt gcttctgaag aggcaaaaca caaactctgg tttgaaactt
     7141 tatacgatga gtggttaaag cagggtaatt agcgttttga aattagctct tgatacttct
     7201 caatcttctg gttccatcgc tttatgggat aagggaaaaa ccatctggtc tgcctgtttt
     7261 gatatccgaa ttactcatag cgagacttta atgcctcaga ttgacacagc tatgaaattt
     7321 tgtggctaca aaccagcgga tatcaagacg gttttagtca ccagcggacc cggctccttt
     7381 acaggactaa gaattgggct tgctacggct aaaggtatag cctatgcttt aaaaatacca
     7441 attttgacct atagcagtct ccagctctat gctcttcagc gatatcattg tgggaaggac
     7501 attctggtgt tacttgatgc taaaatgcaa gaggtatatg ctgcccttta caatgaaaat
     7561 ctggaagaaa tataccctcc acaggtatgc actctggaac atatactatc ctgggaaatt
     7621 agagattgtt atcttatgga aagaggaata aactccttag agctgctttc ccaggaaaaa
     7681 aatgtacctt ttatctcagt tcctgattta cctattagtg cagctggatt gtttttgcta
     7741 caagaaatct tccctcaacc agagatttac aatctggatt atattgccaa tctggaacct
     7801 aagtacctgc gagaatataa cgctcaaatt caaaaacaca aaaactaaga taatccgata
     7861 ttaaaatctc ctaaatttta tataaggata tagcagcttc tttatccata gtgcattata
     7921 ttattacggt gggaatatga tgcttttctg attgacagaa aatagaagat atttttttat
     7981 taaaaacaaa taaaatcaag ggactaaaaa aatgcaaccc tatctaattt atggcggaat
     8041 aattttgacc tttaatcctg aactgccctt actggaaggt tattccttac tggtagaaga
     8101 tggtaaaatt agccaaattc tgaaagatac agagatagaa tctttaccct atgaaagact
     8161 ggatgcccgg ggaaaaattg ttatgccagg attaattaat gcccatcatc atttttatag
     8221 cactttagta actggtttaa ccaaagcatc tccagctaaa gattttctgg gagtgctgga
     8281 aaacctgtgg tggcgtttag ataaacaact gactgcagaa gatatctata tcagtgcgtt
     8341 aatttctgca atgaatgcca ttagaaatgg ctgcactact atttttgacc atcattccag
     8401 tcctggtttt ataaaaggtt ctttatctca aattgctaaa gccatccagg aaagtggaat
     8461 tcgtgcatct ctttgctatg aagtttccga tagagatggt attaataaat gcgaagaagg
     8521 cattctggaa aatgtgacct ggttgcaaca agtctctcag gaacaaaatc ccaggcttaa
     8581 aggacttttt ggaatgcatg cagctttcac tctctctgac gattctctga aagatatagc
     8641 agattgggtg gataaactca actgtggtat tcacattcat gtagctgaag gcgaatatga
     8701 tgaactcttt aatattcagc actatggaca aagagtgatt gaaagactct gtgattttga
     8761 cctcctgaat tgccatagtc ttctggcaca tggcgttcat ctaaatgcca gagaaatgat
     8821 gttgatagaa gaagcaggcg cagcaattat taccaatcct caatccaatc ttaataatgc
     8881 cgtcggaata gcagatatat gcaaaatgaa tgaattaggt atcactgtgg gtttaggaac
     8941 agatgctatg actactaata tgctggaaga actgagagtt ggaatctggg cacaacatct
     9001 aaaacagaat aatccttccg caggttttga agaaatgact aagaccttac ttttgaataa
     9061 tcctataata gctcaaaaat attggggaga taattttggt ttcatagctg aaggagctcc
     9121 tgccgatatt attctgatag attatgaacc acatactcca ctaaatgaag agaattggat
     9181 tggacatata gttaatggca tctctcaagc taatgtaaat acaactatct gtgcgggaga
     9241 aattttgatg tggaatggtc aattgctgtt atctgtggat gaaaatgaag tgcggaaaag
     9301 aggtcgtgaa cttgctaaag ctttatggga aagattttaa ggaagataaa tgtatcacga
     9361 gattttagaa aaagccagag aatatgaagc tgaaagcatt cgttatctaa cggatatgat
     9421 acgcatacca gctttttcca ctaaggaaaa ggaattggct gaatatatcc tgcaagaaat
     9481 gaagaaagct ggatttgatg aagcttttat agaccctctg ggaaatgtca taggaaaaat
     9541 aggtagtgga aaaaatataa ttgcctttga tgctcatata gatactgtct atcctggaga
     9601 cctttcctta tgggatttta acccttttga acctcaggta aaagatggga tgatttgggg
     9661 aagaggagct gttgaccagg aaggtggaat ggcttcaatg cttactgctg ctcgtataat
     9721 aaaagagtta aatctaaatt caaagtttag tatttatttt accggcacag ttatggaaga
     9781 agattgtgat ggactttgct ggcagtatat cataagagaa gataaaatag tgcctgaatt
     9841 ggtagtcctc acggaaccta ctaatatgaa tatctatcgt ggacatcgtg gcaggatgga
     9901 aatgcaggtt tttgtccaag gtcgttcctg tcatgcttca gctccagaaa gaggcgttaa
     9961 tgctatttat aaaattagtc gtattgctct ggaaatagag aagcttaatg aacatttacg
    10021 ctatgatgac tttctcggaa agggcactat tgcagccaca gaagtgcatt ttactggacc
    10081 ttcacaatgt gctattcccg atagtgcata tctgcattta gaccgcagac tcacccaggg
    10141 tgaaaacaaa gaaatagcag tggaagaagt taaagaagcc tgtcaattag ctggagaatc
    10201 agatgcccgg attcaggttt taacctataa tgaaccttca tatactggat tggtctatcc
    10261 cactgaaaaa tactatccca cctgggtcac tcctgcggat tctccctatc ttaaaattgc
    10321 tgaaagagct tatagccaga ctttaggacg aaaacctgta attgataaat ggaccttttc
    10381 tactaatggt gttgctattg caggaatgca tcatataccc tgtattggtc tgggtcctgg
    10441 caatgaagtt tatgctcatg ctcctaatga agcgacgcct gtgatccatc taaccgaagc
    10501 tgcagctttt tatgctgcgc ttatctatgc tttaaatcaa tgagtttttt tcagaactgg
    10561 cgctgtacca attgtggtaa tgaatatgaa ttggatgaag ttcactatct ttgccctcaa
    10621 tgtagcaaaa agtggatagg tggcgttcct ctaatcgggg ttttagaagc cttctttgat
    10681 tatcaagcga ttaaacaaga atgggataaa caacccgatc ctggagtgtt cagtgttctt
    10741 gaggaaaaat tctatcctcc acttccggtt ggtaacacac ccttttttag acctgaaaga
    10801 cttaataaaa acttaaatct acctaattta tttatcaaaa acgatgctct gaatcccagt
    10861 ggttctttaa aagaccgtgc ttctcaatta gtagtcgcag atgctattag attgggagta
    10921 gataaaattg tttgtgcttc cacaggaaat gcagcatcct cacttgcctc tatctgtgca
    10981 gctgcaggaa aagaagctat aatatttgtc cccaaaagta ttcctgaggc aaagctcatt
    11041 cagattaaag tgcataaagc aaaactctat actgtatctg gcacctatga tgatgccttc
    11101 aattctgcgc tggcttattc tgctaataat gattgtttaa atagaaatac cgctttcaat
    11161 cctttaacta tagaggggaa aaaatctgct ggaattgaga tatttcttca attaaacgat
    11221 gtcccagatt ggattattat accagttgga gatggtgtca ttctgggagg tatctataaa
    11281 gcttttaaag atttaaaatc tgctggtata atagagaaat tacctaaact tctttccgtt
    11341 caagcagaaa gttctgatgc tattacttct tactgggaaa caggatccta tcataatgcc
    11401 aatcatcctt acactatagc tgattctatc tcagttaaaa ctccagctaa tgcacactgg
    11461 gcagttaatg ctctatatca aactcaagga aaatcagttc gtgtttccga tgaggaaata
    11521 ctttctgacc agaaatatct ggcacaaatg acgggaatct ttgctgaacc ttcctctgca
    11581 gcagttttct caggattgaa aaaggcttta acccagaaat ggattaaacc ggaagagaag
    11641 attgtcctgc tcattacagg aactggctta aaggatataa atgccgttag attataaacc
    11701 tgcaggaatc tgtgactttc atgtccatat cggcgaaaaa ataggcggat atgaattgcg
    11761 ggatgatttt cctgatctgg atagaatagc tgaacttaat ggtattgctg gaattggagc
    11821 attcgtgacg gaagaatcgg acctatctct aacggtaaag cttagaaaga tgcaagaaaa
    11881 agccagactt aattttaaag gaaaagtaca ctggcaccta actccagttc atacaaaaac
    11941 tgatgaatta tttccgcttc tgcaagaagg atgggactta aaattctata ccacctatcg
    12001 tgaatctgga ttgtactgct cttatgaaca aatagaacac tggatgatag aactatcgga
    12061 tattaaaacc aggatgttag tgcattgtga agatgaccag attataactc aagcttctgc
    12121 agagcatcct tttcgcaccc ctcaagacca tactctcagg agacctgaac tggcagaaat
    12181 agttgccgta gagaaaatct tgaacttagc tataaaacat actcatccca tacatatagt
    12241 tcatatctct tcccctaaag ctgcaatcct ggttaatgaa gcaaagaagg attatccttt
    12301 tataacctgt gaaacagcac ctcattattt aatatataat gaaaatagat tgtctggtat
    12361 aaatgctcat cgctggcttt gcacaccacc attccgaagt gaagaatctc gtggactctt
    12421 agtagaactt ctgcaagatg gatattttga tattttagcc agtgaccatt gtccatttaa
    12481 acttgaagat aaagaccgct ttaaagataa tcttgaactg gtaccttgtg gaattcctgg
    12541 tttggaaact ctttattcca gtatgttcaa caactttgta gaaccaggaa ttatctctca
    12601 agcgagtctg gatgaaatga ccatccataa accgaagact ttaatgtctt gcttttaagg
    12661 gtataaatan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ggtcaggcta
    12781 tttatactga tgacctgaaa ttacacgata tgttatatgc tgtccctgtc tatagtgaac
    12841 tgcctcgtgc aaaactcatc tctcttgatg gttctgctgc cttaaaacag tcaggtgtgg
    12901 ttgctattat caccgctaaa gatattccag gaaatatccg ttacggacag ataatcaagg
    12961 attatcctgt tctggtagat gaagatatca attccacggg agatgtttta gctttaattg
    13021 tagcccatac acgcgagcaa gcaataaccg ctgttccttt tatcaaaata gaacttcagc
    13081 ccttaaaacc tattttagac cctgaagaag cgatgaaacc agatgctgag attcttcacc
    13141 cttttcacgg ttccaatctc accaattatc atcgggtccg tcgtggagat gtagccacag
    13201 cagaaaaaga agctgacctc ataattgaag aagaattttc cacttccaga gtagaacatt
    13261 cctatttaga accagaatca gctatatgtc atttaagacc ggatggagtt atggaagtct
    13321 ttggaagtat gcaacatcct ttttcaactc gtcgtttcgt tgcctccact ttaggagtag
    13381 agctaaagga tgtggaagtt aaacccttaa cctgtggtgg cggtttcggt ggaaaagatg
    13441 atacagctgc attggtttgt gcacgtgcag cgctctgtgc ttggattctt aaaaaaccgg
    13501 ttaaaataac ctattcccgg gaatggagta tgagggaaag ctacaaacgc catccttata
    13561 agatgcaata tagaatggga cttaataaag aaggtcaaat cctttccgtc aaagcacgca
    13621 ttgtggcaga tggtggtgct tattgtagtg ttactccctg ggttacctgg cgttcaacgg
    13681 ttcaaagttg tggatgttat gaggtcccca atgtggaaac tgatgtctat ggagtttata
    13741 ccaataatgt cttttgtggt gctatgcgtg gttttggttc tcctcaaata aattttgcta
    13801 tagaacagct ggtagaaatt gcagcaaaaa agctgggaat ggacgaaatt actttccgtc
    13861 gtaaaaatat ggttaaacag ggttccacta ctataactgg tcaggtgctg gataatcata
    13921 tagtttctct aactcaagtg ctggatgcag tattgaaaga aagcgattat gaaaataaac
    13981 gtaaactctg ttctttgggc aatcccaatc aaaaggaatg gtatggtatt ggactggcta
    14041 tcagctatcg gggtgtaagt cttggtgctg aaggaactga tgtgaattct gcgattttat
    14101 atgttcaacc tgatggttca actttattag aaacaggtgt atttgaaaat ggacagggcg
    14161 cagaatcagc tatgatttta atcgcatcag aacaactggg acttccttta gaacgaatcc
    14221 gttatcgtat gccttccaca tctaatattc ctgatggagg ctcaaccgta gcatctcgtg
    14281 gaactattat gggtggagga gcagtagtta atgcctcaat aattttaaaa cagattatca
    14341 ccgaaaccat acagaagatt acagctaaat ctgttgctca ttttaaagat tctaaactac
    14401 ttgactcttc agataatctt attatgtcct gggatgaagc aataaagcta tgttatgata
    14461 atcaagtcta tccttatgcc tttggagttt tccagcctcc acaaatttcc tgggatgaag
    14521 aaactgggca aggaaatgcc tattttacct gggtgtatgg atgtcaggca gtggaattaa
    14581 aagttaatcc acaaacaggt gcagtggaac ttttgaatat gattgctgct cacgatattg
    14641 gtaaagtgat aaatccttat gctttaaagg gacaatacta cggcggtatg gctatgggtg
    14701 cgggttatgc tctgtttgaa aattgtccct gtgaaaatgg cttagttgtt cctactaatt
    14761 tccataatta tcgtccgcca agaagtataa atttaccgga aatgaaagct atctttatag
    14821 aaaatccaga ccctatttca ccttctggag ctaaaggtat cggtgaacct gctaatgaaa
    14881 ttatggctcc tgctatagct aatgctattt atcacgctac cggtaaacga tatcattctt
    14941 tgcctataag gataaaaata tgattacggt taatggtttc aactactata attatgaccc
    15001 ggaaagaaaa ctttctctct ggttgagaga agacctttct ctcacaggca ctaaaatagg
    15061 ttgcgatatt ggagtttgtg gttcctgtac tgtcctggtt gatggggaac caaaactttc
    15121 ctgccgtctg aaattgaaag atgttgagaa ctgtaatatt atcactatag aaggtatcac
    15181 cttaccagat ggaactttac atcctgttca acaagcattc attgatgcag gtgctattca
    15241 atgtggattt tgcacgcctg gtatggtctt aactgcttat gcgttactta ataaaaatcc
    15301 ccatccaacc aggcaagaag tgcgactggc tattagggga aatctttgtc gttgcaccgg
    15361 ttatcaacag attattgatg ctgttatgct tgccgctgac agactttcct ccaactaaat
    15421 ttaaaaacgc aattgaggaa tgaaatggat aaaaaagaaa taattaaatc aagactttta
    15481 gaattggaaa aactgcagcc agagctttat ggaaaagatt ttctgctcac ctgggaaaat
    15541 agtctggata atttgaaagc cattatggct gtggcagaat taatgcaggg tttgcattgg
    15601 gaaaagaaac ctttccgtat ctttgactat ggtttagccg tgtctatctt tcgtgataac
    15661 agcactcgca ctcgtttcag ttttgcttct gctgttaatg ctctcggttt agctttagca
    15721 gaattggatg aggtaaaatc tcagattgcg catggagaaa ctgttagaga aacagctaat
    15781 atgatatcgt ttctcactga ggttataggt attcgtgatg atatgtatcc cggagaagga
    15841 cactcctata tgttggaagt tgccgaagcg gttaacgatg gattccaaaa tggagttctg
    15901 gctcagcgtc ctatgattct taatcttcaa agcgatttag accatcccac tcaatgtctg
    15961 gctgaccttt taaagttgaa aagttatttt ggagacctca atcagctgaa aggtaaaaag
    16021 atagctatga gctgggcata ttctccttct tacggaaaac cactttctgt tcctcagggt
    16081 ataatcaatc ttatgtctcg cttcggtatg gaagtagtct tagcctatcc agaaggctat
    16141 gacttgctgc ctgaaactgt aaatagcgca caagcttttg ctgagcaaag tggcggttcc
    16201 ttccgtatca ctcattctat gacagatgct tttactgatg ctgatgtggt ctatccaaaa
    16261 tcctgggcac ctatgacggt tatgcaagaa agaacaagat tattaaaaga aggaaatctt
    16321 caggcattgc aggcattgga aaaacaatgc ttagcagaaa atgccagaca tcaagactgg
    16381 gaatgcaccg aggaaatgat gaaacttact cgcaatggca atgcactcta tgaacattgt
    16441 cttcctgccg atatttcagg tgtaagttgt cccaaaggcg aagtagaaaa atctgtcttt
    16501 gaacgctatc gtattcacac ttatcaagaa gcaggatata aaccctttgt catcgctgct
    16561 atgatatttc tttctaaaat aaaaaatcct ctatcccact taaaatcttt aaatatactg
    16621 gagtagtaat gcctaaatta atcactgaaa agaattacaa tatcatcttg aaaaatgcta
    16681 aacgctgtaa agaacgagga attatcctcc ctactattaa acagcagatt aatcctgaaa
    16741 ctattcccaa taacatcaag aatcaattat caaatatagg tttatgggac atcaatcctc
    16801 tgaatctgtt tcgtataacc tggaaaaatg acattaaaac aggtcttttt gggaaaccta
    16861 acttttttga acttccttct tctcttaccg gagttccagc tcgaattatt gggatggtgg
    16921 gaaaatactt tcctactggt gcacataagg tcggtgctgc ctatggttgt ttagtaccac
    16981 ggcttgttag tggaactttt gacccggaaa cgcaaaaagc agtctggccc tcaacaggta
    17041 attattgtcg tggtggtgct tttgattgtg ctcttttagc ctgtccagca gtagcaattt
    17101 tgcccgaaga aatgagtcaa gagcgctttg aatggttacg ccaaattgga gctgaggttt
    17161 atgctacacc cggttgtgaa tctaatgtta aagagattta tgacctttgt gctgaattac
    17221 gaaaagatcc tcgttatgtt attctcaatc aatttgagga attcggtaat cctttctggc
    17281 actatcatgt cactggaaat gctattaaag aagtctggga tatagtaaaa gatgaacata
    17341 gtaattttag tggttttgtt agtgctactg gttccgcagg aactattgct gccggagatt
    17401 atttgaaaaa acatttccct ttgatgaaaa ccatagccgc tgaagctctg gaatgtccca
    17461 ctcttctctt taatggtttt ggtggccatc gtattgaagg tataggagat aaacacatac
    17521 cctgggtgca taatgtccgt aacactgatg ctgttgctgc tatacgggat gaagattgtc
    17581 tccgtctttt ccgtttgttt aatgaaccct caggacaaga atttctcata aaagaaggta
    17641 tcgcagaaga tatcgtccaa aaactacccc tcttaggaat ttcttctatc gccaatcttc
    17701 tggcttctat aaaattatgt aaatattttg agttggggaa agatgatatc atttttactg
    17761 tctttaccga ttctgcagaa ttgtatcaat ccagacttat agagcagcgt aatcttaaag
    17821 gagaatattc tgaaagacag gcagctctgg attgggaagg tacaattatg tcgcaacaga
    17881 ttgattattt ccttgagctt cgctattggg ataaaaagcg cattcataat ttaaaatact
    17941 atacctggat agaacagcaa ggaaaaacct atcaggaaat tctccagcaa tgggatcctg
    18001 catactggca agaaactttt gaagataatc tggaacaact ggataatgca atagaggagt
    18061 ttaatgccct tctctgatat acttaccatt ataccggctt ccggaaaagg taaaagattt
    18121 ggtcaaccca aacataaagc tttgttaaat ggtaaaactt ttactgattg tatcataaat
    18181 accctgaaag aagcggaaat taataatata tacttagcga aagatttcaa tacgaaggat
    18241 atgttatcta ccctgcgttg tgcattaaaa caattaaatc ttcaccgctt ttcgggattt
    18301 ctcattttcc ctattgacca tcctttcgtg caatcttcta ctataaatgc tttatgctct
    18361 acctttcatc aatttccaga aatggtaatt cgtccttctt gcaacggaaa aactggacat
    18421 cctgttctta tcccttcctc tttagacctt tttatagatg ataatgaaga gggtcttgct
    18481 tctattattc gcactcaagc aaaagggatt attgaccttc cagttgaaga taaagggatt
    18541 ctgcttaata ttaaccgtcc tgagaaccta ttatggactt aatggaattg attgccactc
    18601 gtcatagtgt gcgggattat cttcctgatc ccattcctga agaaattctt aaacagattc
    18661 tggaagcagg aagattagca ccttctgcac gaaatcgtca accctggcgt tatattgtct
    18721 ttacttccaa tgatgatatc aaagagttat ccttgcgttg cggtttgatt ggcttatcca
    18781 atatctttat tcgtaaagct ccttgtctaa ttgttgcctg tgctgatacc aattacaatg
    18841 ttcggataaa tcatcaggat tactatctgg tggatactgc tatctcggtc cagcagatgg
    18901 ttttaactgc ctggagcttt ggcattggaa gttgctggat tgctgcattt agtgaaaaaa
    18961 ctctgaaaaa atatctccaa ttacctgatt cctggaggat tgtagcttta cttcctttag
    19021 gctatcctgc agaaaagaaa actctctaca ctcaggcttt aagcgttttc ccaaaaagca
    19081 agaaccgttt atccctggac aagattgtca cctattatca taaaagctca agctgaggtt
    19141 taatcacttt ttttatttga caaacccctc tttatttatt tagtgttaag gaattatttg
    19201 aggtaaaaaa tgaatataaa attaactcgt gaatttgtgc aaaagctccc caaaactgac
    19261 cttcatgtcc atctggatgg tagtgttcgg ataaatacca ttttagattt agcaaaaaaa
    19321 ttcaatatac agcttcctac tatggacccg ggtgaactca gaaaacttat tgtttgtgat
    19381 gaaaatacca gaagtctgga agaatatctg cgtggttttg atatagttaa tcttgttctt
    19441 caaaccaagg aaggtttata ccgcgctgct tatgaactgg cagaggatgc agctaatgaa
    19501 aatgtccgtt atatggaagt gcgcttttct cctattttac acacaaaaaa gggactgaaa
    19561 atgactgaaa tcactcaatc agttatagat ggtttaagac aagcagagcg agaatttccc
    19621 atagagacag gtgttattat ctgcggactt aggaatatgg accctaccac ttcaattaaa
    19681 ttagctgagt tggcagtggc ttttaaaaat agaggagtag tcggttttga tttagctggt
    19741 agcgaattta attatcccgc caaggaacac aaagaagctt ttgagctctc tcttaaaaac
    19801 aatctcaata taactattca cgcaggtgaa gcctatggac ctgatagcat tcatcaagct
    19861 gtgcattatt gtggagcaca tcgtatcgga cacggaactc gtttagtgga agatggtgat
    19921 ttactgaatt atgtcaatga ccatagaatc cccttagaaa tctgcattaa aagcaatttc
    19981 catactaaag cagttcccaa tattcaaagt catcctatag atttctatat tgattatgga
    20041 ttaagagtta ctatcaatac cgacaatcgt actatcagtg ataccactgt caccgatgaa
    20101 tatatgcttg ctatcaacga acttcactta gattatccca ctataaaaaa tgttatctta
    20161 aatggcttca aaagtgcttt cttaccttat aaagaacgag taagattgat aaatgaaact
    20221 ctccgcgagc tggaagaact ggaaaaaagc gaactaaaaa ccactattaa aatgaaggaa
    20281 aatctgtaac tatgtttgac cttatccaga aatcttttac cgaagctgaa aagactttgc
    20341 aagcttttat taccaataat cacaacttag aaaccataga taagattgca gacctgatag
    20401 ctgaagcatt tgagagggat aataaagtga ttatctttgg aaatggcggt tctatgtgcg
    20461 atgctatgca ttttgccgaa gagctggctg gacattttcg ccaaaatcgt aaagctcttc
    20521 ccgccattgc cttatctgat ccttctttta tcacctgtat agctaatgat ttcggatttg
    20581 aagaaatttt cgctcgaggt gtggaagctt atgctcaagc cggagatatt gtggtaggaa
    20641 tttccaccag cggtaattct ctcaatgtca tcaaaggctt acaaaaagct ctaagtcttg
    20701 attgcttcac tgttgcttta ttaggtggaa atggaggaaa aattgctgga acctgcgatt
    20761 ttgaattaat cgttccgtct tttgccacgg atagagtgca ggaaattcat acaatgataa
    20821 tccatatctt attagaaaac atagaaaata ggctttttgc ctatatttaa ttaaaggtac
    20881 ataatgcaaa aaataatctt tactcttatt ttaatttcac tgagtatctt ctgttttgct
    20941 cagggaaatt attacgattc tgtgattgat ctcactggac aaactcttta tacagcttta
    21001 cattcactca tatctaccaa taccaataat agctataatg gttctaaagc ttttcttttc
    21061 cagcaattgg ataatgttaa tggctatgta acctgtatct atactggcca atccttttat
    21121 gtggggtata actacactgg ttcaactaat ccaaatacag aacacattta tgcccaaagc
    21181 tggtttggag ttgagaatga tccagtgcgt cgttctgacc ttcatcatct tttcccttct
    21241 gattccaatg tcaatagtgc ccgtagtaat tatcctcttt tcactgttgc taatcataat
    21301 tctgccaatg tttattattc atatactccg tggcaaagct atcgtggtct tagtgccagt
    21361 ggctataccg tttttgaacc tgctgatgaa tccaagggaa atgtggcacg tgctctctta
    21421 tattttaata cccgttatta tgatactctc actcagcaaa atgtaaatat gatacccgat
    21481 ttagttgtct ggaatactca ggaccctcct gatgcagcag aaattgcacg taataacgct
    21541 atctatgaat ttcaaaataa tcgtaatccc tatgttgacc atccagaatt tgtagaccga
    21601 atctggagta ctccaattac cgatgatgca ataacttccc ctccatctta cctctccctt
    21661 agctctatct cacccaatcc ctttcataat actgcagtta taaatatcaa gagtcaggat
    21721 gtcggtaagg cagaaatctc tatctataat cttaaaggtc aaaaaattcg cacctggaat
    21781 cagactcttc tctctggttc tacggaaata atctgggatg gcacaaactc ttccgggaca
    21841 aaagtccctt ccgggattta tttgataaat attaaaagcg gtggagagac tatcactgct
    21901 aaagttcttt ttaattgatt atctctctat tgcgaaaaag gataaattat gagcctgaat
    21961 tatgaacaaa ttaagtcaca attgcaaagc tataaaccta agtgggaaag taaaaaaact
    22021 caactggaag ctttaactat acctgaagat tttcctttta aagaattttt taatgcctcg
    22081 caagatattt ttcttcaagg atatgagttc gggaaaataa tttcggaaga cccagaattt
    22141 aaatctacac ctatagaatt gctacaaaca ctcaatgctg attatttcgc ccccatcaaa
    22201 ccagaaggtt atcagagaag cttggctaat ccagattaca cagtaaatct atatggtaaa
    22261 gatatgggac agttactttc tgctatttac actcaatatc gcaatacacg cacatatctc
    22321 ttgtttgata actacctcca actggatgaa gatttacatt tattcttaac tctttatgat
    22381 ttagcctctt ctaataatgc caattttgat gactggaaaa aggtatatct ctctgcccgt
    22441 ttagccaata tgtatttaaa atccgccctt caaaatctac tgagactctc tcctgaggtt
    22501 gaccttttcc gtaatattat agaaacttcg gatttaactg atcttcgcta tcttttccgt
    22561 tatggaaatt atatcagtga taatgagttt gccctcgcag attttatggc tcaatatccc
    22621 tcagaggaac tggataagct ggcaaaatat ataattcagt gctggataga tggttttatt
    22681 cgtgccaaaa aagattattc actcaagaaa tatgtaaatc tggttattcc ttgtggtatg
    22741 gaacgccttg ggaagctttt gatagaagaa ttaaaaaggc ttaattttat ccctttagtc
    22801 actcaacctt atactaaagg tgctaatcgt caatacaact atgaccatcg ttttgacatt
    22861 gcactactgc ttgaccgttc ctatgtggat gaaagcctca aaattgcaga agatatcctg
    22921 gaagatctaa aagacctcat tattgaccaa gcaggacctg tctatgttga actttttggt
    22981 gagcttccat tcactcctga aatcaaatct tctactattc aattaagtcc tgagcaggaa
    23041 cagctttata gggaactgag cagtaaaaat tcctttctct atatgaagta ttatcatccc
    23101 gatgaaacaa gttttaccat tatagctttc ccatctcccg aaattggacc gcaattccct
    23161 gaaatctttg ctgatacggt aaaaatcaat acgctggata gtcataaata tgctctcatc
    23221 caacaaaaga taatagatgt gctggataat gctgatgctg tctatgtaaa aggtaaacca
    23281 ggaaatgaaa ccgatattat agttaaatta catcccttat ccgattctga acatcaaact
    23341 aagtttgaaa actgtgtagc agatgttaat atccctgtgg gtgaagtctt cacttctcca
    23401 ttacttaaag gcaccaatgg tactctcttt gtagaagata tttacctgca aaaccttcgc
    23461 tttttcaatc taaaaattca atttgaagat ggctggatta aagattattc ctgcaccaac
    23521 tttcctgatc ctgaagaaag taaaagatat atctaccaaa atctcctcct accacatcag
    23581 actttacctc tgggcgaatt tgctatcggc acgaatacca cagcttatca aatggcgcgt
    23641 aaatacaata ttatttctcg ccttcctatc cttatcatag aaaagatggg acctcatttt
    23701 gccattggtg atacctgctt cagccatgaa gaagaggctc ctcatatcaa ttttttaaac
    23761 ggtaaatcta tgattgctgt agaaaatgaa aaaagtacta ccagaaaaga agaccctata
    23821 aatgcctaca ccaataaaca tatagatatc accctaccct atgaaatgct ggattctatt
    23881 actgcaattg gttacgatgg aactcgcact gatatcatca aaaatggcag atttgtcctc
    23941 cctggtacgg aagaattaaa tatacctctg gatgagggat ggtaattgat ttttgattat
    24001 cctctttctc tatctctttc aatggcttct ctgcgaaggg attctcgttt gtcataaatc
    24061 tttttccctt ttgccagagc cagaataact ttacagagcc ctttttcatt gatttttagt
    24121 tccagaggga ttaaggtcat accttgttct tcaactttag attttaacct gatgatttct
    24181 cttttatgaa gtaatagttt gcgtttgcga ttggggtccg gaacaaaatg ggcagatttt
    24241 ccccagggac tgatatgaag gttgtaaagc cagcattctc catcttcaat tttggcatag
    24301 ctatcgcgaa aattgacatt tcctgcccgg atggatttta tttcacttcc agtcagtact
    24361 attcccgctt ctattttctg caggatataa tagtcgtgtt cagctcttct atttttaatt
    24421 gatttcaatt ttctaattgc tgaagaatat attctttttg ttgtttcgca atagcaataa
    24481 gggcagattc atccacttgc ataagcttcc cactttccaa cacaattttg ccattaacga
    24541 caacatctcg cacattcctt gaactgagag catagacaat atgagaatag ggattatata
    24601 gtggctgact ttccagttcc gaaagactaa ggatacaaat atctgcatct ttaccaattt
    24661 ccagggaacc gcgtttatgg gaaataccaa gagctttggc tgcatcacaa gtcgccattc
    24721 tcacaatgtc cactgctgga agaaaagcag gatcattatt tatcacctta tgcaacttag
    24781 cggtaacatc catttctgat aggatatcca gattattgtt agaggcaact ccatcagtag
    24841 caaagcaaag atttacccta tactttctat aagcagccaa aggaagaata ccagaagcaa
    24901 gtttgagatt gctttcagta cagatagcaa ttgaagcagg aacctctgag agtagctcaa
    24961 tttcttcttc atccatccag actccatgag cataaaccgc tggtaaatca agaattccta
    25021 tatcctttaa ataaaaaact ggtctcaatc cgtgttcttt gatacattgt tctacctctg
    25081 attttgtctc ggaaagatgc atatgcaata atattcctgt atcagctgct acctgagcac
    25141 atttctccag cgtttgacga ctgcaaccat agatggaatg aggtgccaga ttaaaatcca
    25201 cataaggatt atccttatag cgttgtttca attccagcat cttatttcca agttcattaa
    25261 acctttctgg agcactggta ttcccatcta aaagtgcctc tccaataata gcacgcaaac
    25321 caactttagt acaggcatca gctatagccg gcatatcaaa atacatatcg tgaatctggg
    25381 ttatgccatt tttcagcatt tctgctgctc catgaagcga ggcattatag atgaagtttc
    25441 tatttagaat ctttgcttca aggggccaga tataatgatt taaccagatt tccagaggaa
    25501 ggtcatccgc taaaccgcga aaataggtca ttggaagatg agtatgtgca ttaatcaaac
    25561 caggcatcac aatgcaatcc gatgcatcca gggttttagt agcaatatat tccttgcttt
    25621 ctgctggata gatgtcaata atcttgcctt catcaatcgc aatctggtaa ttttccagat
    25681 tcctcatctc ctcatccata caaaggataa tgccaccctt tacgattaaa tcaatctctt
    25741 tattcatagt atttttgcac cttttgagct gcttcagaag caggaaaaat gccctgagga
    25801 gtaataattc cagttataag ttttgccggt gtgatatcaa aagctggatt caaagcagga
    25861 gatgaaggat tagctgtcgg aacaccagcg aactgtttca cttcttcttc gctgcggaat
    25921 tctataggta tatctttgcc gcgaatgcac ccaaggtcaa aagtggaaag tggagcagcc
    25981 acataaaaag gcactctgtg atatcttgcc aaaactgctt tttcataggt tccaacctta
    26041 tttgccacat ctccatttaa acaaacacga tcagcaccag taatcactaa atcaatctct
    26101 ttcttccaga aatagaagcc acaagcacta tccggaatga tagaatgagg aattccttct
    26161 tgttccagtt caaaagctgt gagaagtgca ccctgaaaac gaggacgagt ctcactcaca
    26221 taaacaaaaa tctttttacc ctgacgatga gcaaaacgaa tagcagcaag agctgttccc
    26281 caatccacag tggctaatgc ccctgcatta caatgagtta ggattcttga ctggtcctga
    26341 attatcgcac ttccaatttc tcctatcgtg cgacaatcct cttcactgtg tctggcaaat
    26401 tcctgggctg caagtatggc aacttgccga caatgcttta tatctggaat aaactttatg
    26461 gtctgttcat aaacatagtt taccccatta agcaaatcaa ctgcagtggg acgagtttct
    26521 ataagatgtt ttctcgcttc tctaagagaa acacgaaatc tatcctcggg tgcaacttgc
    26581 gccgctaaag ccatagcaaa agctgctgca gttccaatgg caggagctcc tctaacggtc
    26641 atatctttta tggcttgagc cacctggagg taatctgtga attctacaat ggaaagttcc
    26701 cagggaagtt tgttctggtc aatcgcatac aaacgattat gttcccacca tacactttta
    26761 tattcttttt gatttattat catattattc cgtttttatt tagtttttaa actttctcct
    26821 ctttaacttt gttaattctg ttgtaattcc agattaatcc acccataaga gttaataaac
    26881 tcgcaagcaa acctatcaaa ctcaatctga tactgatagt ataggattct ggagcaaaaa
    26941 ccagttccag tttatgctga cctgctggaa tctgcaatcc acgaaggaca tagttagctg
    27001 gatagatagg aatctcttta tcgtctaact ttgctttcca gccagcggga taataaactt
    27061 cgctaagcac taagaaagaa ggcttatcag tataaacatc ataagcgagc ttatgtaatt
    27121 cattaacggt ttgaaccacg ctgcaggaat caggagcttg cacatcttta atatctgttt
    27181 ccacataagc cagattacga ggattaaaag tctcttccct caaaagtggt aagatagaat
    27241 ctgcgggagc cactttctgt actttatcca caaaccaggc acgaggcaaa gctctattat
    27301 ttttatatat aatcacattg tcacttccat caaagactgg atggatatta actaataatg
    27361 aatcataggg caggtgttca ggatgcaaca tatatttaac tgatagcata tccattatat
    27421 tagttggttt ctctcgtgga atttctccct tttcaggact aaaaattcca ataagataat
    27481 cacgccattc accaggtcct ttaacgctat cctgcagata gttcattaaa gcatcataac
    27541 gagccagttt ggcagcagaa taacctgtaa ctatttgatg atgatatgcc cattctccag
    27601 ttgtcttggg taaacgtgca tcactgataa tattttgttc aattggataa atccgatagt
    27661 tgctatcatc agccagtaaa tattcatcat aatcctgagc tgcaaagcgg tctttatatt
    27721 gctgaacagg ttgcagattc tcttttttga gaaacttacc tgtataggta tagaggtcaa
    27781 taaagacaat taccgacatc agaaccacaa aaatcatttg aggcaatttc ttcacggttt
    27841 taagatatgc caatccaata ctgacagtta atagtagtag actcaatatt ccactggtat
    27901 atagcatatt aagccgtgta gcacgaatat cagcaggaat ctcagtcata ttgtattttg
    27961 ccaactgttg cttttcttgt gcactggcaa aaggtaaccc tccaaaaata ggtttagcca
    28021 gaatcaacca caaaagaaag attattccac aaatccagaa agccttgaaa agtcctttgc
    28081 tcaattgctt tctttcctct tccgtcaaac ttaaaatagt gtccaatccc agtcctgcca
    28141 taattactgc cagaatctga accatcacca gaatcataga aggtactcgg aatttattga
    28201 aataaggtag atattttaaa aatagattag agagatgggg tgtagcacta ccgaaagaca
    28261 taattaaaaa gagtagagag ctgattccaa gaaaggtagc tgttttacga tgttttttac
    28321 cgcacaaagc tataattgcc agagccagga ctacaatccc aaaatagtta taagtctgag
    28381 tgaagggcat ataaccccag taatcaggac tcacacctcc aaaaaaatta ggaataatca
    28441 aggtaacgat ttccagagga tgaaaactcc attgctgagc atagctggtt tccagacccc
    28501 cggaactacc acgaggggta tatttactgt attcccaagt gcttaaatag ggattcataa
    28561 ccgctaaaac tgttatcaaa aatgccagaa taagcaatcc agtaaattgc cagaatttct
    28621 ttaaatcctt agctttaagg cttactatca attgccaaat ccaatacaat cctacaaata
    28681 gatataaata ataggtgatt tgcggatgat tctctcgcaa ttgcataatc aaggttgtgc
    28741 tcaagaatcc aagtccaagc aaccctggct ttttccttaa atgaattaaa gcccagataa
    28801 cccagggaat atacattata gctcggaatt tagtattgtg tcctatctct aataatccca
    28861 tccagtggca ggaaaacata aaggctactg cccctaaaaa acaagcccag atatccagtt
    28921 tgagatagcg tagcaataaa aacattccca gagcacctac aaatagcagg aatatcctcc
    28981 agttgataac cttatctgtt atcctggtga tgttttccag aaaaggatag cgattgggaa
    29041 aagatatcat ataagaaggc atcccggaaa acatactctg agtccagaga gcagggtcct
    29101 tgtgggtctt attatactca attatctgat gtgcagctcc ttcccattga gctgtatccg
    29161 aagctggagg aaccatatgc tgaaaagcta ctggaaaata gagcagacta acaaatacaa
    29221 acaaaaatat catcaaaacc cagggcagcc atttcttttg agaaagagtc tctccaggtt
    29281 cgattacact aactggcaca gaagagggtt tgcctttttc ctgcaatttt ttctttacct
    29341 cttgctgagt taccatcttt ttcttctctt gtttcccaac tggggaaatt ttgggattga
    29401 caattttctt agccatataa tcctcaaatc agatattgtt attacctttg aattatgctg
    29461 cttttattgg tcaagaataa attgcacagc atagttattt aaataacgga gttgcactat
    29521 gatatttgaa ttccagaaaa gaatatacgg ctatgaatgt gatgtgtatg gacacctaaa
    29581 caatgctaat tatctccaat tactggaagc tgcacgggtg gaagctatga taaagatggg
    29641 gatgtccatt aaaagaatga gagagctcaa tctgcagatt cttatccgaa atttcaacct
    29701 ggactataaa agagctgtcc agcatgagga tttaatcact atccgcagct ggtttgatga
    29761 aataagcaga ataaaacaac actggattca gcagatttat gattccagcg gcaggctctg
    29821 ctttgaagct cagatgctgg gagtttttgc cagtgaagga agagcgcaac gcttaccagt
    29881 ggaagtcttt gaggtcttcc atctttacca agaatctaaa gctccatctt aaataagcta
    29941 attaaaacta tttactctta gcatagtggt actttattat cggtaaaggc ttgtatcata
    30001 gtaagtaaaa gtaaattgtt cttgacagtt ccagtcaaag ataaaaaagg atttttctat
    30061 aaaaatatga taaaaggagc ttgaatgtcc agtattgcgg acaatattaa gagaataaaa
    30121 gaacaaactg tagaaaaact aatttccttg ggaagacctg aagatgacat aactattgta
    30181 gctgtcacga aaactcatcc agttgcagta atagaggaag ctctgcgaaa tggcatttct
    30241 catatcggag aaaacaaggt gcaagaagca atacataaaa tcccatttat aaccgttcct
    30301 tatgaaggtt tccattttat aggtcatctc caatccaata aaataaacaa tctcttaaaa
    30361 ctaaaaccaa tgctcattca gtccatagat agtctatata tcgctcaaca acttaatcgt
    30421 tcactgggac gaaaaaatat cactcaggat attttaattc aggttaatac tactgctgaa
    30481 accactaaaa gtggagtcaa cccagaaaat gccatagata tgatctggca gattgctgct
    30541 ttgccttgtc tgaatattaa aggattaatg accatcggaa tgcttagtcc taacccggaa
    30601 aacacccgtc cctacttcag aaaattatgg aaattgaaag agaaaataga ggctgaaaaa
    30661 atacctgaag ttcaaatgaa atatatttct atgggaatga gtgaagatta cctgatagct
    30721 ctggaagaag ggtccaatat gctacgtctg ggcactatta tctttggtac cagaaattat
    30781 ggaggaacaa attgaactat ctgattttaa ttctaatcat tatagctgcc tatttttatg
    30841 gttgcatctc ttccgctcgt gttgtagcaa aaaccttccg ttctttaaat atatataaag
    30901 ttggcactgg cttagctgat acagaaaata tctttgttaa tgtgagtcgt cctctgggag
    30961 tcctggtagg actactggac attcttaaag ctttcttctt tttacttgta atagaatatc
    31021 tcgtgcgctg gcttaatttt tatggaattg tagagggggc tgaccttctt tataacaaaa
    31081 acattatgtt actttatgga ctggcaatgc tggttggaca ttgtcttcct gctaccaata
    31141 acttcaaagg tggaagagga atcttcacct atagtgggta tttgctctat tttgttcccg
    31201 ctcctatgat tatttcttta attgttgccg gagtaattac cttcatctgg aaacaagttc
    31261 gttttgctca gtatgttatt gttattcttc ccgtgctttt aactcatatt ttttatgcct
    31321 ttattcctac ttttagaagt aatttacctc ctcattttat cgttataatc tgggctattg
    31381 caattattat gggtgtactt aatttcatat tatccaaaag gttaggcgaa atatagaatg
    31441 tccaaccatg cttctgaagc tctcataaaa gaaaaaaaga cctctttagt tgatgtggaa
    31501 agcaagacca aaactttctt aagccctata gatattataa ccattgctta ttgtgcctgg
    31561 attattatct atatgataat tggtattttt gtgggaagag cagaacaacc ggcaattcat
    31621 cttccacgat ttatcagcat tataactgct gtgttactta tggcttggct ggaacgcaat
    31681 tatgcttccg ttatgaatcc taagctattg cattttcttc agtttatcag aagtatctat
    31741 cctgttctcc tttttactta tttttatact tctctgcatt cggtttgtcg cattctctgt
    31801 cctgagtggc tggacccttt cttcagcaat atagattata aaatctttgg ttattatcct
    31861 tcaatggtct ggggacaaaa atatgacaac tggtttgccc aggaattttt ccattttgcc
    31921 tatttttgct attatcctat gattgcagga ttacctacct atctctattt taaaaataaa
    31981 gaaggcttta aagagctgat tttcaatctt acctttgtct tttatttttg ctattttttc
    32041 tatagtctcg ttccggttgt tggaggtcgt tactttcctg aaggttatca attagctcat
    32101 tcctatcgtt atggcttttt caccagactt atggctttta tctataatca tagtggacac
    32161 tggggtggtg ctttcccaag ttctcatatt gcaataacta tagtgctcac tattgcgggt
    32221 ttaaaatatg tacgtaaatg gggttatgta ttctgtgtaa tagctttctt tctatctatc
    32281 tctacggttt actgccatta tcattggttt attgatgcag tggcagggat tgtaaccggg
    32341 attgcaggtt actttgtagc taattggatt agacaaaaac tggagaaaga accaaaatga
    32401 agcgactact aactttacta ttcctctgtt gctctattac tatttttgct cagaattcgg
    32461 atatgcctat cattcagagt tatgattatg ctcttcctga aaacaatgtt tcccctattg
    32521 ccataggtat gggtggatta aatgtcacct atgcaggaga tttctattcc agttattcca
    32581 atcctgcttt aattggagac aatgatttta gcgtattgat gggtgcattc aggataaaaa
    32641 gtgatgaagc ggtgaatttt gctgaagctg ctcaatttag caatattttg aagcccaagc
    32701 agttaaagta tatttctctt ctggcaaaac aaacagcatg gacttatcaa ccagtggctt
    32761 ctgtacatat tgttgaacct cctgaaaccg gagtgggata tcgctattat gactataaac
    32821 tggataaagt tcaagttacc ttagccggca gtgatgattc ctggccacct atccgcttcg
    32881 gtttaaatct taaatactta acgggaaggc tggtctatct tgtctctacc aatccaaatt
    32941 ccttcattga tgacaaactc aatggctttt ctactgattt aggttttacc ctccagacgg
    33001 gaagtttcac ctatggtttg gcagcttatg atattttctc tcgcttatac tgggaacatt
    33061 acaattcaga agctattcaa agcagagtag ctatgggtat tcaatacgga acagataatt
    33121 tcaacttaaa tctgggattg cagggtaaaa ttgctcaatc cactgatacc acctatcatc
    33181 ttggaggaca atatatctgg acctggggtt cgcaatatta tagtgtggaa agtgcaaata
    33241 aagcactggc gtttagagct ggactatata gtcacaactt ttatggtact cagaatatca
    33301 attataccat tggtggcgga tataactaca atattttccg ttttgacttt tccttgaaca
    33361 ataaaggtat gaaacttaaa gatagtgaat tacttatatc gctgggtgtg ggaatgccct
    33421 aaactatggg taacttaact ttcctcaatg caggattcct ctttttctta gctgcaacta
    33481 tacttccatt aattatctgg ttgctggcta agaaaaaacc aaaacaggtt gttttcccta
    33541 ctatccgttt catcaaagcc agtcaagaac aggaaaaaaa gaaaacccga tttaaaaata
    33601 tccttctact tatcatacgt atgctcatta ttctgctggt aactttagct gctaccagac
    33661 ctttattttc ttcttctcag ttgaaacctt ctgctaatca tcctcccact gctttagcaa
    33721 ttttattgga tacctcatat agtatggatt atgtttatga ttctcgcagc aatttagacc
    33781 gagcaaagga tgcaatcaaa aagattaatt cactctccaa ttctgatgac cgtttaatcc
    33841 tggtcacttc agatgaaaac tggaatcgcc ttcattctca gatatatgca ggctctattc
    33901 cagaagatat catagaacca ataagcgtta cctacgaacc tttaaccatt tcccttatgc
    33961 tggaacaggc aaaaagccgc ttacaggaaa ctcagtttgc caataaggaa atctacctga
    34021 taaccgatgg tcaaaaacag gaatatcctt ccccttctga tattaccctc caagtgatta
    34081 acatcgctga gaacgaagat ttttcaaatc taagttgttc tcaagcacag gtcttacctc
    34141 aactggcaga aaaatccaga agacaaacta ttgagtttaa actcaccaat catggaaata
    34201 aagaccgtca ggaagtgctg gttagaacag tgctgggaga agcaaaggta ggagagaaat
    34261 tcgcccacct tcctgctcgt cagaccgtaa atcaaactat ggtcatagac ctgcaaaaag
    34321 atggctggca gagtggatat gtggaagtgg ttgatgacca tctggaacaa gataatcgca
    34381 gttatttttc gttccctttt tatctttatc ctcaaattgc ggtcatcacc cagaacaggt
    34441 ctttacctcc agctttatat tctctcttaa gagtttatgc aggctccgag ggcaaaatta
    34501 atttaattga accgcagcaa cttaatcttt ctatgctcaa tcaataccaa tcctatgttt
    34561 ttttaggcac cccttctttt ccacctcaga tgcgagaagt cataaatact ttacagagtt
    34621 ccaatcgtgg tgctcttttc tgtctttccg ctaatctttc cactgaatat cgcgcatttc
    34681 tggaaaatat atttaaggtc aaatttggtg cttggcaatc tcagccaaaa tctataactt
    34741 atgttaatcc ccatcatttt accacttctc ttgttgccgg aaaaagtctt caattcaata
    34801 acatttcaga tttctggcat ttagaaggga gggaaataaa tgcacttctg gctggttcgg
    34861 gagagacctt tgccttagcc aaagataatt ttatcctctg gaactttgac cctggttcag
    34921 aacgctctac atttttgctg gatgcagctt ttcctgtctt cgcttatcgt tgtttggaac
    34981 ataccagttc atctattgct accggagaaa aatatactgt cggagatata ataaacactc
    35041 acagtgtaat aactccagct ggtgaaaact tgttattttc caatcgtcct tataagcttt
    35101 ctcaaccggg aatttatatt ctacgaaaag ctgatggaac ggaaacggtt atatcggtgg
    35161 aagctcccag acatgaaagt gaatttattc caatggatta tcaagaattg aaaaatgtcc
    35221 agctcttagg tgaggactgg caggataaga tattccatac acggctgggt tatgaattat
    35281 ggaaaatcct gctggctata gccttagctc tggtcattct ggaaattatt ctggttaaaa
    35341 gtgaggaact gcgctccatt ccggaagcag ttcccagttc aggaagttaa ttatgatatt
    35401 agaaaatata aaggaccctt cttccatccg aaaattaaat acggaagaaa tgaacctcct
    35461 ggcaaaagaa gtccgcaaac gcatcattga tgttgtgtcc aaaactggag gacatatcgc
    35521 tgccagttta ggtgtggttg aattgaccat cgccttgctc aatgtgtttg atcccttaaa
    35581 agaccgtata gtctgggatg taggtcatca aacctatacc tggaaaatcc ttactggaag
    35641 aaatgataga tttgaaacgc tccgttctct taatggattg agtggattta gcaaccgaaa
    35701 tgaaagccct tatgatgctt ttaccacagg tcatagcagc acttctatct ctgcagcttt
    35761 gggtatggct tgtggaagag acctcaatcg tgaaactgga cattgtattg ccatcattgg
    35821 agatggagca ttaactggtg gtatgagctt tgaagcttta aatcatgcag ggcaactcca
    35881 gaaagataaa ttcctcgtaa ttttaaatga taacgctatg tccatctcac caaatgtggg
    35941 tggtctgcaa aaatatctgg caagaatgct ggcaagccgt ccttataaca ctctgaaaca
    36001 acaaatctgg gacctcagtt ccaccttacc tgcaaatcta cgtagaacct tcattctcgg
    36061 agctcaaaaa cttgaagaat caatgatgaa catactggtc cccaatatca tctttgagga
    36121 tttaggattt aaatatgtgg gtcccataga tggtcacgat ataaatttaa tctgtactat
    36181 gcttcgtcgg attcaaagac atatggtggg tccggttttt cttcatgttg tcactcaaaa
    36241 agggaaaggt tatctacccg cagaaaaaga aaccgtcctc ttccatggcg tggaaccttt
    36301 tgatgcagaa agtggaactt tacctgctaa tggaaagaaa agctggagcg aagtctttgg
    36361 aaacaccatt tgcgatatag cggaaaataa tcctgaagtt gtggctatta cagcagctat
    36421 gtcctctggc acaggtttaa ccccttttga aaaaagattc cccaaaagat tctttgatgt
    36481 gggaatagca gaacaacatt ctgtgacctt tgccgctggc ttggcatgca agggaataaa
    36541 acccttcgtt gccatttaca gcaccttctt acaaagagct atagatcagg ttattcacga
    36601 tgtagctctg ccaaaattac cagtcgtctt ttgtattgac cgtgctgggt tagtaggaga
    36661 agatgggccc actcatcatg gaacctttga tatctcctta ttaaattcca tccctaatct
    36721 aatgattttt tctcctgcca atgatgagga attgattgct atgataaact ttgctgccac
    36781 ttataaggaa ggtcccatag ctatccgtta tcctcgtggt tctgcttttc acagtgaaga
    36841 agttgttacc gaatttatcc ctggtaaagc tcaaatagtt aaagaaggtg aaaatattgc
    36901 tctactttcc gaagggggag cctggcaaat tgctctggga acctggaact tattaaatag
    36961 caagggatta aatccctggc ttataaatct ccgttgcttg aaacccttag atgaggactt
    37021 gctaaaaaat ctcggaaaaa cctgctccca tatctttacc tttgaaacca atgtggtaat
    37081 cggtggagtc ggtgctcgca taggacaatt gctggaagaa tcaccctgta aagtaattaa
    37141 ctttggttat cctgatatct ttgtcccgca tggaaagata gaacagctta atgaattgat
    37201 tggtttcact cccgctcatc tttcggaaga gatacaaaga catctaagtt tataaatggc
    37261 aaatctttcc gagcctatct gtgctcttat tacacctccg ggttatgctg ccataaatgt
    37321 tgtaagaatc agtggaaaag gaagcattgg cattgtagca caatatttta agccccgcaa
    37381 aaaactactc aattgccctt ctcatagggt ggtatttggc accttttatt cccgcgaagg
    37441 aagagccata gaccaggtgc tttgcacagt ttttagagca cctcaaagct ataccggcga
    37501 agatgtggta gaaatatcct cgcatggaaa tccacaaatt gcctcccaaa tcctggaaaa
    37561 tctacttctg gatgcaagaa tggctaatcc aggagaattt actttgcgag cagttttaaa
    37621 tggaaaaatg gacctccctc aagcagaagc agttaatgac ctcgtttttg cctccggtgc
    37681 tatggctgaa tcagctgctt taatgcaggt gcagggaatt ctctcacagc atttacaaaa
    37741 tttgctaaat gatatatcgg acacaagatt acgctgtgaa ttagccattg atttcgcaga
    37801 tcaggaccta cctcctgtag atcccgaaga tttgaaaaat agaatatccc atattttaaa
    37861 acaagcagaa gagctgtatg cagaaggtgt ttcgcaggga aaatatatca gagaaggcat
    37921 taaaatcagt ctggtaggtg cacctaattc tggtaaatcc tctattttca atgcatttct
    37981 aaagtataat cgggcaattg taaacccaca tcctggtact accagagatt acctggaaga
    38041 aagagtcaac ttacagggct ataccttgat cttatatgat actgccggat tgagaatcag
    38101 ctccgatgaa gtggaacagg aaggtattga aagaaccaga gagcttatgc aaaactctga
    38161 tttaattctc tatcttcttc ctgtagacca ggaaattgac tggaaagaaa ttgatgaact
    38221 tccccctgaa ataaagaata aaattctctg ggtagcaagt aaatatgacc taataaatcc
    38281 tgatttttct actgttaaaa actttgataa tttgaatgga caatcacctg attccgctat
    38341 tcctgcttct gtatatgcaa tagaaggtct ggaaaaactc cagcaagcca ttcttaatca
    38401 ttttgctcta cctcaaaaat gggttgaccg tcccttagta actaatgcac gtcatcttgc
    38461 tgctttatcc agagccattt cttctcttaa aaatgctctc caatctattg ataatcaagc
    38521 aggttatgaa tttaccgcct ttgatttaat tgccgccagc caagctctgg aagaaattct
    38581 gggtatcgtt accaccgagg acttactcac caatattttc aataacttct gcattggaaa
    38641 ataatatctc acacttaatt aaaatatcca gctctcacat ataatcccta aaaatcactt
    38701 ttaaaataga ttatttacac ctttatctta tctcttcata atctatcttc ctcttatttt
    38761 tcgcttttct atcccttatt tctttattat catatcatta cctgtcatta ttgtcctttt
    38821 tatattaacc tgggaagatg tataccttcc cccaatttct ctcctggctc tctcttggct
    38881 cttttgctca taaagtgcta agagagagtc aattaggagt tttgcaattt aaaatatgcc
    38941 taactcgctg atatataata caattagcta tcacttttct gactttttgt gtccaataac
    39001 ctttagattt gtatgataat taattatgaa ttcctgagct gcatccatat gaaatccttg
    39061 aattttgtaa aaggaccggg taaaaaatgt ctatcttttt gatagatatg gagctctaaa
    39121 ttttctattg ccataatata gcagtttaaa agaatggtaa ataagaaata agtaaaaaag
    39181 gaggtgatta tggtccaata tttcaaagtt caagagcagc gttttgtccc tgcaatgtca
    39241 ccgaatgaag ctttctggat tcatctggat aatcctaatg atgaagaaat caaacaattg
    39301 attgaacgct tcaatttgga agaagactat atcaccgacc tccaggatgc tgatgagaac
    39361 agccgtatgg aatatgatgg aggtgcagtt ctcattatac ttcgggtgcc tttatattac
    39421 cgtcatagat ctgccactat tccttttgct acagctccat taggaataat tgcggtacag
    39481 gacaagctca taactgtttc tttctttgcc actgaagtgc tcactcaata ccttgaaggt
    39541 aaacataaac cttttaatat cacacaacag agttttctac tccaaatagc tctccgcact
    39601 gctatctatt ttctgaagtt cttgaaagaa atcatacgcc gttccaataa catagaaact
    39661 gaattatacc agagtatgag aaataaagag atcatccgtc ttttaagatt ggaaaaaagt
    39721 ttagtctatt tctctacctc cttacaatca aatgagataa ttctggaaag gatgcaacgc
    39781 tcacggtggt tgaatcaaga ccctgatgca gaagatatga ttgaagatgt tattattgaa
    39841 aatcgtcaag ctattgagat gactaatgtc cattccagta tcttaagcag tatgatggat
    39901 gcttttgctt ctatcatttc caacaactta aatatcagaa tgaaattcct gacttcagta
    39961 agtatcgtcc tgatgctacc aactctggtg gcaagtatct ggggtatgaa tgtagcatta
    40021 ccacttcaag aaagtaaata tgcttttctc attttgatgg gaatctcagc agtagcatcc
    40081 accctcgtgg tattatactt tatccataaa aatatgtttt aaatagaggt tatctatgta
    40141 taaattaagc gtctatgatt actttagtgc tgctcaccga cttcgtggat atgaaggtcc
    40201 ttgcagcaat gttcatggac ataactggaa agttaaggtt actttccaga cagatacact
    40261 tgattctctt ggaatggcta tagattttaa agtcttaaaa gcgaagctct gggaaatctt
    40321 aaatcaactt gaccacacct ttttaaatga actgcccata tttaaagata ttaatcctac
    40381 ttctgagaat attgccaagt acatttttga aagcataagc gaatccttta aaggtagctc
    40441 gctatcactt ttagaagtgg aactgcaaga atccgataaa gttagtgtaa tatatagcaa
    40501 tgcttagatt cgtgcaatcc tattttgtta aaagtgcagt tgaacccaaa gattatcctg
    40561 ccagtgccta ccctgaattc gctgttgcag gaagaagtaa tgttggtaaa tccaccttaa
    40621 tcaatctatt aacaggctat catcaactcg ctaaaaccag ctcacatcct ggtaaaacga
    40681 gacttttaaa cttcttcctt attcggtgga aagatgatga agctcaaaaa gaaggacatt
    40741 tacagttaac tgacctccct ggttatggtt atgcagaagt ccccttagcg gaacaggaaa
    40801 aatggcgtaa aatgataaac aactactttg accagcgaga aatgttaaaa ggaataattg
    40861 ttctggtaga tataagatta gcagccgatc ctaaagatat ccagctcctg gaaatgttaa
    40921 aacttagagg aatagattac tgtgtcgtcg ctactaaagc agataaaata ccaaagacca
    40981 aacttaatcg cacgctacaa catctggcta aaggacttgg gttgaaagat aaagatatct
    41041 ttccggtgtc tgccctaaaa aagacaggat tggaacctct ttataattgg ataggagcac
    41101 atctgatata aatatgaccc gtgactttga tgtaatcgtt gtaggaggag gtcattcagg
    41161 tattgaagca gctctggctt ctgcccgaag aggtgctaaa acagcacttt ttgtcctgaa
    41221 actggaaact ataggcagaa tgagttgcaa tccttccatt ggaggtcctg ctaaaggaca
    41281 tcttgctaag gaaattgatg ctctgggtgg agaacttggt tatgtagcag acctttccgg
    41341 cattcatttt cgtatgctaa accgaagtaa gggtccagct gtctgggctc ctcgtgctca
    41401 aaatgatagg ctgctctatt cattattaat gcgacagcac ttagaagaac aggataatct
    41461 cagcttgata gaagcaagtg tttctgaact tttggtagaa aaaggaagta ttcagggtgt
    41521 tagaatcttg caaggaaagg aatattttgc tcctcaagta atccttgcga ctggaacttt
    41581 tctcaatgga ctcatccata tgggaaatat ctctttccct ggtggacgta gtggagaacc
    41641 tgcttcagaa gctctttcta acagcttact aaagtatgga ttgaaacttg gacgctttaa
    41701 gaccggcact cctccccgaa ttgatatgag aaccctgaac tataatcttt tagaagaaca
    41761 accaggagac cctgaaccaa tgggtttttc tcagtatcgg gaagtagaac tttctaataa
    41821 agttagctgt tatatcaccc atactaccac cgaaacacat aatattatcc aacaaaatct
    41881 gcagaaatcc tcgctctatg gaggcatcat aaaaggtatt ggacctcgtt attgtccttc
    41941 cattgaagat aaaatagtga aattccccca gcgcgaaagc catcatatct ttattgaacc
    42001 cgaaggattg gacacctgcg aaggttatgt gaatggactt tctacttcct tgcctcctga
    42061 tattcaagaa aaattgctac atagcattcc aggtctggaa aatgctcaga taatgcgata
    42121 tgcctatgct attgaatatg attatattaa cccagaagaa attcttcctt cactggaatg
    42181 cagaaaaatc aaaggtctgt ggttagccgg tcaaattaat ggcacatctg gttatgaaga
    42241 agctgctgct caaggcatta tggctggaat taatgctact cttgctctgg aaggcaaaga
    42301 acccatcatc ctcagtcgca ctcaagcata tattggtgtc cttatagatg acctggttac
    42361 ctgtggaact aatgaaccct atcgtatgtt tacttcccgc gctgaatacc gtttgctcct
    42421 tcgtcaggat aattgtgatg aacgattaat gcctctgggt tatcaattag gattggtctc
    42481 agatgttcgc tggcaaaaat ttaaacatac tatggaattg aaagaacggg aaatggaaag
    42541 gttgaaaacc gagaagagca tcaatcatcc tgacctgaaa gaaccacaaa aatttgctgt
    42601 ccttttaaaa agaccagata tcaatataga aaagctcaaa ctttacggtt ataaaccatc
    42661 tttagaagta actccagata ttcttcgccg ctgcgagttg gaattaaaat atgaaggcta
    42721 tctgcaacgc gcagaagaag aaatgcaacg cttccgtgct tctgaaaata tcttcctctc
    42781 taatgatata gattattatt ctatcaatgc acttgcctgg gaagctcgtg aaaaactaat
    42841 gaaaatccgc cctcataacc ttgctcaagc aatgcgtatt ccaggagtta actattctga
    42901 taccgctgct cttttgctct ggctaaagaa acaacaaaaa attgagagaa aagctgaatg
    42961 aaagcagtag caataattcc tgctcgctat gatagcaccc gttttcccgg gaaagctctg
    43021 gcaaaacttc tttctaaacc tcttattcaa tgggtctggg aagcagtaat taatgccaat
    43081 ctttttgatc aggtaatagt cgctactgat tctatacaga ttaaagaagc tgcagagaat
    43141 tttggtgcta tagctattct caccagaaaa gaacatccaa gtggaactga ccgtattgct
    43201 gaagtagcag aaatgcttga ttgcgatatc gtagttaatg tgcaaggtga tgaaccttta
    43261 attaatcaaa aagctctttc ccctttactt catgtttttg agaattctcc tatccagata
    43321 gcctcactaa tgactaaaat taaagattta aaacaacttt ctaatcctaa tctggttaaa
    43381 gtggtagtag ataatgcttc taacgctctc tatttctctc gttctgccat accattcaat
    43441 cgtgataaca tccattttga agattacttt caacatattg gagtatatgc atatcgtcgt
    43501 caaaccttaa tgcaattagt gagactacct gcaggaaaac tggaacaggt ggaaaaactt
    43561 gaacaattaa gagcactgga aaacggaatc cctatccgga tggtcactac agaatataat
    43621 ggtatgggag tggatacttt agaggattta cttaatctgg aaattttatt aaaagagatg
    43681 aactatgaag tttaagagaa tttgtattgt aatcctgtcc ctgacaatta gttgtatcta
    43741 ttctactccc ttttctgtct tttacacagg agatactcat ggtgtttatg aagctcaatt
    43801 ggataaagaa cgaggaactc ttatgggtgg ctatctggtt ctggaagaag tattaaatca
    43861 aaaaagaacg gagagtaaga atagcattta tgtagatacg ggagaccaac aaaccggcac
    43921 tattttctcc tcactggtag aagatggaat ctatggtgga gcggttattg aagtcttcaa
    43981 ccagctcgca cttgatgcca gtgtgtttgg aaatcatgaa tttgactttt cctatactaa
    44041 caccagagac ctggcaaaaa aagctaatta tccctttgtc tgcacgaatt taatagataa
    44101 aactacaaga cagagtgttt ccggaatacc ctatgctatt ctggaaagaa ataatataaa
    44161 agtcggaatc tttggcatca ctctggaaaa attgcctgaa aaagtcaaag ccgaaaatgt
    44221 tagtagtgta accatccttc cacctattga ggctatcaat aaatatctgg aagaagttga
    44281 taaaaagact gatttaatca ttgtcctaac tcaccaaggt tttgaagcgg atagcttgct
    44341 tgccgttaat ctggataata gagttgacct cattattgga ggacacgacc atataaaagc
    44401 agaaaagccc cactttataa acggtaagta tctgctctat agtggttctc atctaaatta
    44461 tctgggttta gctgaactgg aagttgaaga tgacaaaata acctcactta aaaatcagct
    44521 tattcctctg gaaataccag taaaggaatt tgatacacct ctggcaaaat atatccagga
    44581 aaagactgtg aaaatagaag aagagatgtc taaggtaatt ggttttattc cagaagattg
    44641 gattccggat aaatatcaat ccactaccct ttctcgctgg atagccagtg ccttaaagac
    44701 tgaatacgaa agaacttatc atcctgatat ggctatcatc aacaatggag gcttacgaaa
    44761 agtaattcct gctggaccgg taacacttcg tgatttgaat gaattgctac cttttaataa
    44821 tacagtggta gtcttttctt gctatggtaa ggatttatta actttcttcc agttaaatga
    44881 acttcattca caggaaaaac cttttgatat ttgcgaatat accaggctgg attcaattga
    44941 atcagataaa atctaccgga taatctcaca tgattatatt gctggacagt gggataaata
    45001 cctcggtttt aaaccttttg atatctatga taccggagaa cctttcttag atgctattat
    45061 tcatcaggtt caacttcaat tttcacccat aaacgaaaaa gattgggatt aaaaaaatgg
    45121 aaacaaccaa aaatatcaat aagaaagaaa acttaaccga ggaagaagca atctctatcg
    45181 gcaaatatga cccgctatgg ataattaatg ccttttccag aagtttctat gaaggaaatc
    45241 cagcggcaat tgtagctcta tccgatttcc cctcagataa aaaaatgcaa aaactggcaa
    45301 aagagtttaa tctttctgaa accgctttct tttgcttgag aagtcctgat ttagctcaaa
    45361 tcagatggtt ttctcccacc caggaaattt ctctctgtgg tcatgcaact ttggcaacag
    45421 cctcagtaat tttttctcac ctttacccag agaaagataa gtttagtttc ataaccagta
    45481 atggtatgat tgagacagaa aaatataacc atttaataca gctaacctta ccacttgagg
    45541 aagtaatccc gttcatcaca gaggaaaaag ttctgaaagc attagggaaa actaagccag
    45601 cagaaagtta tcttgctcct gaaagtcata atcttatcct tatctacgac aatgtaaaaa
    45661 agattaaatc tcttaagcca gatttcccca ctttgctttc tttcaaggat ttgcctttta
    45721 ctggtatcgc agtgtctgct ttagatgaaa atggctatgt ctgccgttat tttgctccct
    45781 gggaaggcat taatgaagat cctgtcactg gttctgcaca gacttatctt gcgccttttt
    45841 ggtcaaaaag attgaataaa aaattgttag ctgtgcgtca gatctcttct cggggtggct
    45901 caatggaagt aaaagtgcta tcatcttacc tccttgtgcg tggaactact tatacctgct
    45961 tttatggtaa tttgaattct ggctggagag aatgaaaggc tctgagaaaa ccatttttac
    46021 tacaaaaact tttccttgac aaagccttaa taatttaatg cttagaaata aataaaactt
    46081 agaggtcatc tgaatggaaa ttataattga agagcttaaa ttcccatctg atgccaatat
    46141 cattctggga cagagtcatt ttattaaaag tgtggaagac ctttacgaaa ttatggtcaa
    46201 cagcgttcca ggcatcaagt ttggtttagc tttttgcgaa gcttccggac cctgtcttat
    46261 tcgtaaagag ggaactgaca atgaattgat ggaatgtgcc atagaaaata tgcatcgtct
    46321 gggagcaggt cattcatttc tcatagttat gcagaatgct ttccctatca atgtgctcaa
    46381 cgctatcaag aactgtcctg aagtggtaaa tatcttttgt gctacagcta atccggtaca
    46441 ggtaattctg gctaaaacag aacagggaaa tggtattctg ggtgtaattg atggaaattc
    46501 acctaaaggt attgaactgg atacagatat tacgcataga aaaagattcc tccggaacct
    46561 gggatataag cgctaaatga attatcgcac ctttattgcc ctggaacctc ctaaatctgt
    46621 ctttctgaac ctaaaagaga agctcaatac ttttaaatct actcagggtg taaattgggt
    46681 aaaagaacag aacttgcact taactctact ttttctggga gatgtagata gcaccagaat
    46741 taatgaatta tctggtatcc tggaacaagc aacagaaata gctgctcctt tcaatctttc
    46801 ccttcaaggt ctggaactat ttccctataa ggagccaaga cttgtttggg ctaccctttt
    46861 agaacaggat gattctttat tccagtggca taaatctcta cttaaagagg ttcgtaaagc
    46921 aggttttgaa cctgatataa aaccgttaaa acttcatatc acgctgggac gtattaaaac
    46981 taaacttccc gttagcaaag aaagagaaat cctggaaagt aaggtagata aggatttctt
    47041 tagctatgat acaatcactc tctatcgtag catcttaaaa ccggaaggtc cagtttatca
    47101 aattttagac caatataatt tatcttaaag gaagatatta tgcaggataa aaacaaagaa
    47161 gtggctttaa agactgccat atcacaactg gaaaagaaat atggcgtggg cactcttatg
    47221 cgtttgggag atcagcccct gcaacaagtg gatgttattc caactggcgc ttttaatctg
    47281 gatatagctc tgggaattgg aggtattcca cgtggacgca taactgaaat ttacggtcct
    47341 gaagccagtg gtaagactac acttgctttg catattgcgg cagaagcaca aaaacttggt
    47401 ggaatcgtcg cttttattga tgcagaacat gctttagata ctgcttatgc caaaaattta
    47461 ggtgtccaag tggaagacct attactttct caacctgatg gaggagaaca agccctggaa
    47521 atatgcgaaa ctttggttcg tagctctgcc atagatttgg ttataattga ttctgttgcc
    47581 gctctggttc ctaaagcaga aattgaagga gatatgggtg atagtcatgt aggtttgcaa
    47641 gcaagactta tgagtcaggc tctaagaaaa cttacctcta tcgtgtccaa atccaatact
    47701 gctgtagtct ttatcaatca aaccagaatg aaaattggca ccgctgcttt tatgaatcca
    47761 gaaactactt ccggaggtgt agcactcaaa ttttatgctt ctgttcggat ggaagtacgc
    47821 tttgcgggag cgataaaatc aagtggaccg gatgcagaag ttatcggtgc taagacccgg
    47881 gttaaaatcg ttaaaaataa acttgctcca ccttttaaaa cagtggaatt tcctattatc
    47941 tttggaaaag gcatctccaa tactgacatt ataatggata tggcaataga ccttggaatc
    48001 attaagaaaa gtggttcctg gctatcctat ggtgatttaa aactgggaca gggtattgaa
    48061 aagaccaggc aatatcttaa tgaaaaccct gctttgttag aagaaatatc caatctggtg
    48121 aaacagaatg taaaacctga aaagatgttt gaatctccta caacagatga aactgaggat
    48181 atcatccaag aataaatatc ttgctaccat agaattagaa gatttaccct ggggaatctt
    48241 acccattaag cagatatcct ctctctatcc ttctgattat gagggagaaa taagcgagag
    48301 tgaagcaaat caaatcatta agatgcttga ggattatgcc tttaatcttt tagtaaaata
    48361 cttagctaaa gaagaacata gtcaatacca atgcgttctt tatctgcaga gaaagggatt
    48421 ttgttcagaa atagttcagg atgctatcca ccgttgtttg gagttaaact atcttaatga
    48481 ccagcgtttt gccgaacttt taatctcttc ctatataaaa agaaaagcca gtaaacgagc
    48541 tattacggct aaactttctg caaaccatat acccataaat gtctggaaac ctctgttgga
    48601 agaactttat actgaagaag aaaaagccag taatctggaa gaattattga aaaaatacta
    48661 ttctactcat tcaaatttac cacctgaaaa actgaaagaa agagcttttg gatatctctt
    48721 tcgtaaaggt tttgacccag acgatatacg agatgcaata gaaaatcttt cttaaatata
    48781 gtctctttat ttaattatga taggaatgag tattctgcca gtattcacat tcctctctgg
    48841 aaagcacata actttccaga ttccaattaa tgagctgtcc tttcaatact tccagaaccg
    48901 gatattcagt gtaaaaatga cccgcatcca caataggaat ttttgcttcc atcaaattat
    48961 gatagcccac atcaccggtt atatagagtt cggcagattg ttcagctaac ctaatcaaag
    49021 aacttccact acctccacaa actgctattt tttgaattga agtggataag ggtaatcctg
    49081 ctgtccatag tttcggattg ggacatccaa gtttatcttt tatccaggat agcgtttcac
    49141 taagtgttaa aggttctggt agaattcctg tcatccccaa gccaaaaaga ggttgagggg
    49201 ttgttactgg gaaataatac agcaaaggcg tctcataagg atgagagatt ttaattgcag
    49261 aaacaacatc aaatagcctg gattcctgta ccataaactc cagttcttct tctgctactt
    49321 gcgtaaattt atcgtcctct tttgacgata taaaaggtct ggctccttct aatggtttaa
    49381 aagtcccttt aattggatga cgagtggaac aataaatata attgccaacc cttcctccac
    49441 cggctttaaa gaccgcatct gctacatttt cagtataacc tgcaggaacg gtaactgaaa
    49501 gatgaaacca tttttcgcct tgttcagagg ataaagattc cagattttcc aaacccaaaa
    49561 ctgtagccag agcataatta gttccttgag gtgcaacatc aagattggta tgcattgaaa
    49621 ttacatttat ttggttctct accagggata ataaaagcgg gtcggttata tgctttaaag
    49681 aatgaaatat cactggatga tgagccagaa ttatatttgc tcctaattcc agagcttttt
    49741 gcattgcctt aaaagttaca tccagagtaa taaggacttt tgttacttcc cttttagaat
    49801 ctccaactaa taaacctata ttatcccatt cataagcctg agaaaaagga gcaaattcat
    49861 ttaaacgctg aagtaattcc tctataatca ttctcttatc tccttacatc ttatatttta
    49921 ttctaattat gcatatcttc atttagtcaa gttcaatatc tttccagacc aataaagaaa
    49981 atatagaatt taggcaatag attctgtggt ttgttataaa aatgtctctc ataatagata
    50041 aagatagcgg tctctaagtt tataggaatt attccttttc ttttttgata aatcaatctt
    50101 ttttagcctg cactttaaat gcgtgcagaa ttctattttt ctctgaattg cttatctatc
    50161 atccagataa atatctcttt tatcatcatt tgaattacct taattggtta actttttgtt
    50221 acttagcctc ctcttgacga tttctttagt aaaaaggtaa gcaagagcta agagagagct
    50281 aagaaagaac tcgtaactta ttgaaattta taattataat atgcaatgta aattcgtgta
    50341 atgaaggaga agatgtcttc accttctgag ctttgcagaa gctttttgtc tctttatatt
    50401 tttaagttat agaaattatc atcggtaaca ctggaaaagc tttactgctt tgcgatacaa
    50461 ggatattctg tcccttaaca ctaatatttt tcattctata aaactatttg aactgctata
    50521 agtaaataag taaaataaac taaatttaaa attatacttg tctaatctca taaatacatt
    50581 aatttaaata tataaagaaa tatttaaagg agagaaaaat gaaaaagtca ataatacttg
    50641 cagtactctt acttgtctct attggattat cctttgctca accatatttt ggttttcgtg
    50701 gaggattgaa tgtaacaaat gtaaaaatta ctgatgatga tagtgatgaa gctaaaactg
    50761 atgaccgtta cgggtatcat ggtggacttt ttatgcaata tcctttaggt aaatatttca
    50821 ttattgaacc ctctctactt tattctcaaa ggggatatga gagaaccatt gaaatcccgt
    50881 tatttgagga tatcaaaacc aaagttaata tggactatat tgaattgcct cttgcattga
    50941 aatttgatat caatatcagt gatgttcata ttcaaccagc agtagctcca agaataggct
    51001 atgcaatcgt tactcagtat ctggtaaatg aggaagaggc agatttccca aatgacccta
    51061 tattattgga ttatggagtt gactttggct tggacattac ttttttaaat cagtttctta
    51121 tcggagccag atattctctc ggtttagcag atatttatga tgatgataat gaggatatca
    51181 ctaccactaa taatggtctg atgataagct taggtgtgat gttttagaaa taagtagaag
    51241 gagagtattc tccaattata tcctaaaatg taaataataa cagattcatt cttttccgcg
    51301 gggatgacct ttaagatagg cttttttaat gtcatccaga gttaggtgag tataaatagc
    51361 ggttgtttca agattggaat gtccgagcat ttcttgaaga gcacgaaggt cagctccgcg
    51421 ggaaaggaga tgagtagcaa agctatgtct aatagtgtgt ggagaataac ctttttgtcg
    51481 tgcaatgatt tggaaataat ggtgtaaaat tttgaataat tgtctgtggt caaaatcttt
    51541 gccacttttg gtgagaaaaa gtttatggga actatattct tgctttaact tgtctctcac
    51601 cggaagatat gcttctatgg cttctattgc tggctttcct ataggtacga ttctttcttt
    51661 attaccctta cctttaacac gcaccagagc tcgtttcaag tcaatgtcat ttagctctaa
    51721 atctgccagt tcagagatcc gaagtccaga agaatagagc aattccagaa tagcacgatt
    51781 tctcacacca aagatgctgg attgatcagg cagatttagc aaaataagca tttcttcttc
    51841 cgtaaaatag tgtggtagac ccttttcaaa cttaggtctt tttattttat ccataggatt
    51901 tttggaaatg cgattggtaa gttttaacca ggagaaaaag ctctgtaaag cagctgcttt
    51961 tcttgccaga ctgcgattgc taacttgttg tttatgaagg tgaagaaacc agtcccgaat
    52021 catttgtgga gagatttgtt caagctggat attaccatct tcaaaatatc tttccaaaaa
    52081 attataaaat tgactcaaat ccttcatata ggcagtaata gtgcatggtg atttcccttc
    52141 accgcgtata tattgttgat atagttcttg ttccagagtc ataagtttat ccctaatatg
    52201 cattttttac ttgcctgttt gtttgtcgag aaaaatttag ttttagtaga aggagtgatg
    52261 atgaatcatt atatcgcaga tagtgccaga ttgggaaaag aaattaaagt gggtggtaat
    52321 actataatta tggatgatgc tgtaatcggt aattattgtc agattggaaa taatgttgtt
    52381 attcatcctg gtacttatat tggagataat gtaagaattg atgataatac ggtaatagga
    52441 aaaaaaccac ttacttcacc tcgtagcatt tttaaaaccc ctcaagaatt gaaacctgcg
    52501 attattggcg ctaattccca gataggagca aatgtaatta tatatgctca gtgtgaaata
    52561 ggagaatcca atatgatagc tgatctggca actatccggg aaaatgtaaa gattggagat
    52621 ttaaatatta ttggacggaa tgtaactatt gaaaactatg ttcaaatcgg ttcccgcaat
    52681 aaattggaaa ccaattgcta tataactgct tattcagaaa ttgaggatta ctgttttgtt
    52741 ggaccctgtg ttgcaacaag taatgataat tatatggcaa gagatccaga acgcttcaat
    52801 cactttaaag gggttactat gaaacgcgga gcacgtatcg gtgttcatgc taccattcta
    52861 cccggtataa ttatagaaga agatgcttgt gttgctgctg gctcagtagt aactcataat
    52921 gttcctgcag gaaaagtggt tctgggaagt cctgctcgta ttttacgcga tgtacctgaa
    52981 gctcaacttt taaagaataa cctggataaa tcgtgaaagc tttactaatt ataccaacct
    53041 acaacgaaat agctaatata aaaaatatta ttactgcttc gctgtctcaa tcacctgacc
    53101 tttatatttt agttatagat gataattctc ccgatggaac agctaaagca gtgaaagaaa
    53161 tgatggaaaa tgagccacat atcaatctaa tagaacgtcc caaaaagatg ggcttgggtt
    53221 ctgcttatgt tcaaggtttt aaatatgctt taaataacga ctatgattat gtcctggaga
    53281 tggatgctga tttttctcat aatccagcgg acatacctcg tttattggaa gcagcaaaga
    53341 aatatgatgt ggtaattggc tcacgctatt gtcagggagt gaacataatc aattggccct
    53401 tccggcgttt acttatcagc tattttgctt ccaaatatgt gcgtataatt acaggtatgc
    53461 ctatcaaaga cccgaccagt ggctttaaat gttttcgccg aagggttttg gaagcaatag
    53521 atttagatag catcctttcc gatggttatg ctttccagat tgaaatgaat ttccgagcct
    53581 gggttaaagg ctttactatc tatgaaatcc ctatagtatt tacggaaagg agagatggag
    53641 tttccaagat gagtcgtcag attgtctggg aagcagcctg gatggtatgg cgcctacaga
    53701 taatgaagct tttaggaaaa attaaatagc tttgaggttt attatgaaaa aagtatggat
    53761 aactacatta ttaatgatag ccttactgtc cctttttgct caaccgacca taccgagagt
    53821 ttatgtgcaa aaactggttt tagaggatgg gacaaatcct tttataactt ggtataagga
    53881 ccaaacttcc ccagaatatc tatttagagc ctggatttgg gaaagacctg atgagatatt
    53941 gagcactgaa acacatacct atcatcatct ggcaatcact caggttggag atgggaagaa
    54001 agttcctttt atggtggtta gtcgtgtcaa tttaggcaat tttcccagtc aatggcatgc
    54061 tggagagact cttcacctgg aactaacaca caaagaaacg ggacaaaagg tagaatggga
    54121 tgtctatatt cctgaaggaa cagccactat tatgctatta gacactcctc agattattcc
    54181 cccttataaa gaagtaaaat ccgagaaaat aatacctaaa actgataata gttccgagaa
    54241 gccaaaagaa aataacagca gctgtagtaa ctgcccgcac tgtcattgac ggaactaact
    54301 aatgttagaa cgcattaaca caccttttga gcggcctgaa gacaaagaat ttgatcgtgc
    54361 tcttcgtcct cgtactctaa atgaatttat cggacaaaca catataaagc agcttctgga
    54421 tatctctata aaagctgcaa aattgaggaa agaatcttta gaccatattc ttctctacgg
    54481 tcctccaggt ttaggaaaga ccaccttagc aggaattatt gctaatgaat tgggagttaa
    54541 tatcaccgta agtagtggtc ctgtgatgga gaaaccaagt gatttagctg gaattctcac
    54601 taatttacaa cggcatgagg tgctctttat tgatgaaatt caccgcctca gtcatatcat
    54661 agaagaatat atctatcctg caatggaaga tttccagatg gaaattatcc tggataatgg
    54721 tcccagtgca agaactctgc gtatcagtat agaacctttc actcttattg gagcgacaac
    54781 tcgtgcagga ttgctgactc cacctttaag agatcggttt ggcatcattc ttcgtctgga
    54841 ttattatgac cttgattcaa ttaatcagat tatccgacgg tctgctaata tcttaggaat
    54901 tccagcagaa gatgaaggaa ttgctgaact tgctcatcgt agtagaggaa caccacgtat
    54961 tgcaaataga ttattgagaa gagtgcgtga ttttgctcag attatgggaa atggagttat
    55021 tactcgggac atagcattac aagcattgga aatgctccag gttgataatg aaggattgga
    55081 tgaaatggat aaaagattgc tttctactat aattgaatat tatagaggtg gacccgtagg
    55141 tattaaaact ttagctacag ctgtaggaga agatcccgga acaattgaag agatttttga
    55201 accttatctg gttcagcaag gcttcctgga acgaacccct caaggtagaa aagcaactta
    55261 taaagcttat caacatctgg gcataatccc tccaaatcct cagacagaaa tcttctaatt
    55321 atttttactt cagcttcaga ttattgtaga tgattcccag aaaaggttat aaagcaaata
    55381 tcaccagtaa cctggtcaat cagattcttc atctaatcat aggtgctgcc accagtatta
    55441 ttgcttcccg tgtcctggga cctcagggaa tgggatatgt ggcttatgcc atattgattt
    55501 tcactatact gggtaatttc ggtcacttcg gtttaaacaa tgcagttatc tactttaaaa
    55561 aaagaaaaaa agttaatccc actcacctaa ataatgtaaa tgtaacatta ttaatcctat
    55621 ttgcagtagc tatcagttct gttctcttag tcctacgaag taacagatgg tttctaagtg
    55681 attacaacta cctttatatt ctgggtggaa taatatatat tttctcaaat ttgcttttta
    55741 ctcactaccg ttcgtggttc gtagctgatg aactaattag ggaaagcaat cgcttcatta
    55801 tcactgcttt ttttatcaaa agtgctatta tcatcctact ctggttgacc agaaccttaa
    55861 atcctctgac ctatttttat gcttctgtcc tgggaatgtt aatcaatgcc atatttttgg
    55921 gaactcatac aggagaaggt tttagacctg agatagactt agctttgctc aaagcagaat
    55981 attcttatgg tggaataata tttttagccg cttgttgtga ttatctacat cttagagttg
    56041 accaattgat gataaaacag atgctgggaa ccagttttct gggggtatat tctattgcag
    56101 taactctggc agaacttatg tttttacttc ccgcaagtat caataccgca cttttaggaa
    56161 agctttataa tactgatgaa aaacaaaaat tcagacaggt tatggctcag acatataagc
    56221 taaccttctg gatttgttgc ttattagcac ttattggtat ccccttaagt ttacttatcc
    56281 ccttcttcta tggtaaggct tttgcaggag caattataag cactcagatt cttttaatag
    56341 gcactatttt ttccagctgt gctcaagtct caatgcaata ttttttcagt acaggaaaac
    56401 cgcagataca tcttctcact acttcctgct ctttattaat taacattgct ttaaatttat
    56461 ggttaattcc cctcaaagga attactggtg ccgccatcgc atcctgcatt tcttatttta
    56521 tctatggact gttttactta atcgtcttta taaaaaaaga ggagttctcc ttgcacgaat
    56581 tattaagtat ttctttatcc gatttaaagc ttctctggag ttctaaatga aaaccgattt
    56641 atttaatggc tatttttggt atcaaaacaa actttttaac gatttaccac cagacttttt
    56701 cccctcagaa tataatccgg agacctatac ttccattctg aaagaattaa atggctgttt
    56761 cagattaata gttgaaggta atgatttcct tattgcagct gtggatagag tacgctctct
    56821 tcctcttttc ttttaccaaa agggaaagga tttattcatt gaccctgatg catttgagct
    56881 aaaaaaactt ctgaataatc ctgaattaga taatttaata tttaccgagt ttttgcttac
    56941 tgctcattgc tgtcaaaatg gtactctcta ccctgaaata aaacaactgg aagcaggaca
    57001 gtttcttatc tggcacaaat cagaagccag gttggagcta agggattatt atatttatca
    57061 gcatctcttt gaacccatag agaatccaat agaggcaatg gatgagatgc accaacatat
    57121 aattcaacgc ttgattgata gcgctcacaa tcgcacttta gttattcctt taagcggtgg
    57181 atatgattct cgtaatatag cttatatgct aaaaagagca gattataaaa atgttatctg
    57241 tttcacttat ggaaaacctt atgacggaga ttgtagcact tcccgtaaag tagctaaatt
    57301 tcttaatttt ccctggatta tggtggaaaa cacacgtcca atgtggtatg aagctttcca
    57361 tagtgaagaa atgctccaat atttccgttt tagcacaaat ctgagttcca gttttcatgt
    57421 tcaggattgg cttgccgtaa aaattcttaa ggaaaaagaa cttatccctt cagatgctat
    57481 ctttgttcca ggtcattctg gaggagctct tcaaggtcag taccttcctt taatatttga
    57541 aaatagagat aaaatgacct caaaagagct gatacagact atcctacggc aattttacca
    57601 tttatgggaa taccccatgg aaaaattata cagccttttt agtgatagaa ttggcagcta
    57661 tcttaccatt cccgatacgg tgctaactga gattgctaca tcaatctatg atgaatgggc
    57721 ttggagacaa tgggaatcaa aatttatcgt taattctgtc cgcgtttacg agttcttcaa
    57781 ctacgaatgg agaatcccac tctgggataa tgagtttatg gagttctggc aaagaatacc
    57841 tttctcacag aggacgcacc gtcagttatt aaaacaatat ctccaaaagt accaaccaat
    57901 tcctgttccc gcttaccatg actattcttt tactcgaaga atcaaaaaca aatatgctcg
    57961 catcactgtc ggtaatatta tgactcttgg ctatggtaga tttcttgatt ataaagatag
    58021 agacgcttat ttaaacacta aaattgcatc tctgttagtt cctgaactcc attatcctga
    58081 atttattaat cctgagcttc ccattctgaa agcacaaatt aacgctattc aggctttaat
    58141 ctatataaaa gaactggtct ctggtaatct ggataatata actttaagca agcaattctg
    58201 aatagagctg ataattttcc ggatcaaaac aataaaaata gacctccagc tcaccatttt
    58261 cataaaggtc tttttttacc gtatcaattg ctatcctggc tgcttgttct ttaggaaaac
    58321 cgtaaatccc agtggaaata ttgggaaaag ctattgattg tataccatat tgtctggcta
    58381 attccagact tctacgataa caggaggcta aggtctctgc ttcgttagag ttacctcctc
    58441 tccagatagg acctacagta tggataacat atctggcttt gagattatat cctttagtaa
    58501 tttttgcatc acctggatta caaccgccaa gtgttctaca ttcttccaga agttttggtc
    58561 ctgcagcacg atggattgct ccatcaactc caccacctcc aagcaatgaa ctattagctg
    58621 catttacgat agcatcacct tcaaacttag ttatatcccc ataaatgaga tgaatcataa
    58681 aactttcctt aaattcccag tctttgacag gcatctcgtg ctgcttcttg ttgggcacct
    58741 tttttggtgc tgcctttccc tttacctaat acctcatttt ttaagcgagc ttccacgata
    58801 aaggttttat tatgctctgg tccttcttct gctacggtta tataaactgg tgggggttga
    58861 ttacgagatt gcatatattc ctgaaggata cttttgtagt tgaccagttc attacgattg
    58921 acaacactct cataatcatt cataatatta tttttaatga atctcatagc ggcagaaatt
    58981 ccgctatcca gatagatagc acagataaga gcttccattg catcactgag gatggaaggt
    59041 ttttttgctc ctccagcttg ttgttcttcc tggctaagaa gtaagtattt tcccaggtct
    59101 aaagcattag ccttgagagt gagataggtc tcggaaacaa ttttagattt aagtttgctt
    59161 aactttcctt cttgttcttc aggatgtctg ttaaatagct cacgagatac cccaaaaccc
    59221 aggatggcat ctcctaaaaa ctccattctt tcaaagggag aaggagcctg ggactcagag
    59281 taattacgat taagataaga tttatgtgtt agagcagctt tcaaaagatt aatatcatga
    59341 aaattgtagt ctatcttctt ttgtaattgt ttaaggctct tttcccattt aggatatagt
    59401 tctttgatac gttttccatt gataaagtct ataaaacgct gaaagaactt atttacaccc
    59461 ttctctttac gaagctcttt cattcttgtg gaaattcacc tccacaatac cttagctata
    59521 tttctttata atgagagctg aattatgtcc accaaatccc aaagaattgg aaagagctac
    59581 attcacatta cagttttgag ctttattagg aacataatcc aaatcgcaat caggatcggg
    59641 attctcaaga ttaatcgtgg gatgaattac cccagtttct atggatttaa cacatgcgat
    59701 tgcctcaata gccgcagctg ctccaagcat atgtcctacc atagatttag ttgaactaat
    59761 cttcaattta taggcataat ccccaaagca tttctttaga gaaagagtct cacctttatc
    59821 attcaacgga gtagaagtcc catgtgcatt aacatattgt atatcctcag gttgaattcc
    59881 tgcatctgct atagctcttt tcattgcttc cgcacttcct gaaccatctt caagtggtgc
    59941 tgtgatatgg aaagcatcat ctgtggctgc ataacctaca atttctgcat atatctttgc
    60001 acctcttgcc ttagcatgtt ccaattcttc aagaactaaa aaacctgctc cttcactcat
    60061 aacgaatcca tctcgttctg catcaaaagg acgagaagct ttggtcggtt catcattgcg
    60121 agtggaaagt gcacgcaaag cacagaatcc tccaacggaa aaagcagtaa ctgcagcttc
    60181 agcgccacca ctgataatga tatcagcatc tccatattga atagcacgaa atgcagttcc
    60241 taaagcatga ttggcactgg cacaggcact ggtaatgcta aaattaattc cttgaaaacc
    60301 attttctatg gcaatatgag cagcggcaat attggaaatc atcttaggaa tgaaaaaggg
    60361 actgattcta cgaggaccaa gttcatgatt cttgatacat tcctcttcaa aagtataaat
    60421 accaccgata cctacacctg taataactcc aattctccag ttgtcaccag tataaggcat
    60481 tagacccgca tctttaattg cttctcttga agctactaaa gcatactggg tataagtatc
    60541 catttttcgg acttctttgg gatcaaagtg ttcctcaggc ttgaagtttt tcacctgagc
    60601 cgcaatctta acaggtaaag cggaagtatc aaaattcttg attaaatcaa caccagaaac
    60661 tccctggact aaattactcc aggtttcttc tatattattt cctactgggg ttaaagcccc
    60721 taaaccggtt attactactc tttttttcat tattaagacc tcttataaag aagattaata
    60781 aacctcatcc cctacaaata cttcacccaa cattaaataa ataaataagt gtagtgatgt
    60841 aagaaattga atcaatcctg ataaccgaag ctatcaggat tgatttttag ttgaggttaa
    60901 gaagccaatt tctccttcag ataatcaatt gcttgtccca cagtctgaag tttgtcttgg
    60961 tcttccagtg gaatagtaat ctggaaggca tcttcaaatg ccataacaag ttccactgta
    61021 tccagggaat cggcacgtaa gtcttcaatg aagttagctg agggaacaat ctccgattct
    61081 tccacattca gcttttccat tatgatttgt ttaactttgg cttcaatggc ttcactatcc
    61141 atttttcctc ctcttatttt cgtgttctct ttcttagggt aaaattcatt gtcacctcaa
    61201 tgcataacca gtccaccatc tatagctatg gtctgaccgg ttatataagc actctcttca
    61261 gaagctaaga aaagacaaag cttagctaca tcctcaggtt ttcccatttt ctgcaaaggt
    61321 atagctttgg cataaccttc tactactgat tctggaagct ttgctgtcat ctcagtctct
    61381 ataaaacctg gtgcaacagc gttcactcta atatttctgg atgcaaactc tttagcacaa
    61441 ctcttagtaa aggctatgat tccaccttta gaggcagcat aattagcctg tccagcattt
    61501 cccataattc caatcacact ggcaatatta atgatagaac ccgatcttgc tttcatcata
    61561 tatttaaaca ctttctgagt gcagataaag gtacccgtta gattaatttc caaaaccatt
    61621 cgccattctt cttctctcat ccgtaaaagt aaattatcac gagtgattcc agcattatta
    61681 attaaacagt ctattttacc tctttcttct ataatagcag aaaagacctc atccatttga
    61741 gaagaattgg tgacatcacc tacataacca gaaactgagt atcctttatc ttgcaacttc
    61801 tgcattgctt catccaccac ttgctgattg atgtctatta ttactggatg agcaccttgt
    61861 ccagcaaagg cttcagctat ggcaaaacct atcccgcgag ctgaacctgt gatgactaca
    61921 gtcatattct caaaatcata tttaaccaag attaaaactc cttatccttt aaaagcgtca
    61981 tcaattgctt tccaatcttc tattttttcc atattaaaaa ctttaacatc tctgtcaata
    62041 ttctttatca ttccgcttaa aactttctgg ggaccaaatt caataaaata tcccactcca
    62101 tcctgaatca ttcttctaac acaatccacc catagaacag gggaaataac ttgttttgcc
    62161 agtttttctt tgcttatctg tcctgaagta ttaggcaagg catccacatt agagattaga
    62221 ggaatagtgg catctttaaa aggaatgttt tccatctcct gagctaacca taaactggct
    62281 ttatgaataa ggggggtatg aaaaggacca ccaacaatta gaggaacaac tctttttgca
    62341 ccttttgctt ttgccagttc gcttgctttt gcaactcctg attcagttcc agaaataacc
    62401 gtctgagctg gtgtattaaa attaactgct ttgactaann nnnnnnnnnn nnnnnnnnnn
    62461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    62521 nnnnnnnnnn nnnnnnnnaa aacatcttct ggaccttctg ccataatttg tcttagatta
    62581 gtttgatgtc ttgcatcaaa ttcatccaat acagcagcaa attctgggta agaagagata
    62641 aaatcctgcg ccatccctac atattgtgca ccctgtccgg gaaaaacaaa agctatcttc
    62701 atatctacac ctcagaatct tgccagaata gcaccccagg ttagaccagc accaaaggaa
    62761 gtaagaataa ccaggtctcc acgacgtatc ttcttatctt taatcgcttc atcaatagca
    62821 ataggtatag tagctgagga tgtattggca tatttttcaa tgtttactat aactttattc
    62881 ataggaaaat gtagcttatc cgctaaactt tctataatac gaatattagc ttggtgagga
    62941 acaatccaat caatatcttt agcttccaat ttattacgac gcaatagctc atcagtggcg
    63001 gcatacatag agcgaatagc atttttgaag atacgattgc cttccatata aactgtgtga
    63061 agattctttt caaccgtttc tggactggca ggcattctag aacctcctgc aggttgatat
    63121 aaaaggtcac tctggcttcc atcggcatac ataactgaat caatgatacg tgagatatca
    63181 tttctttcag ctcgggatac gatggttgcc ccagctccat caccaaaaag gacgcaggta
    63241 ctacggtctt tgaaattaat agtcttgctt aaaacttcaa ctccaataac cagaatatta
    63301 ttctttgtcc cattttctat aaaattttta gccacatcaa ccgaataaag aaatccggtg
    63361 caacctgcag aaaggtcaaa acaggggata tttttcattc ctagcttttt ctgaagtata
    63421 caagctgtag aaggataggc atgatctcca gtgactgttc ccacaataat catttcaatt
    63481 tgtcgatatt tcaatggaga agcttccaat gctctttgag cggcaatata agctaaatca
    63541 ctggcagctt cttccggttc tgcatagtgg cgttctacca ttccggtacg tgtgcgaatc
    63601 cattcatcag atgtatctac tatcttctct aaatcataat tggtcatcac tcttttcgga
    63661 acataactgc caaaggaagt gaattttgca ttatgaatag gcataatgtt tacctctctc
    63721 taaaataatc cttgctgtgt tgaacaaatc cagattggac tgttcttaca gcaaaacgaa
    63781 tagcattttt tatcgctttg gcatcactac tgccatgaca aactatactt gtgccattta
    63841 gacccaaaag taaggcacca ccaaattcag tatgatcaag ttttctcttc aaaaaactat
    63901 aggcaggaaa cgataatatt gctccaattt tcgctatcca atccttttca agttgctcct
    63961 ttaacatatc aaataccgct ctaaccacac cttcaatcgt ttttaaaacc acattcccta
    64021 caaaaccatc gcatactatc acttcaaatt taccactgag tatctcttta ccttctatat
    64081 taccgataaa attaaggtct ttcgctgcac ttaatagctc ataggcttca cgagtaagtg
    64141 cattaccttt agtgctttct tctccaatat ttaaaagacc tacacgagga tttggtttat
    64201 gatagcaatg ggtaaaataa atgcttccta actgagcaaa ttgcagtaga ttttcagcat
    64261 cacaatcaac attggcgccc atatccagta gaattgcagg ttgttctatg gtagtgggaa
    64321 gagcggcagc aatagcagga cgagatactc ctttaatcct gccataggca agtaaagaag
    64381 cagccataaa agctccagaa ttgccagcac ttaacgcagc atcagcctct ccatttttgt
    64441 gtaattctac tgtcttaacc attgaagaat ctttcttcat tctaactgcc tctgcggcat
    64501 ggtcatgcat ctctattctt tcactcgcat gatagattct aattctatta tggtcatagt
    64561 aatacttatc cagttcagct tgcaatatat gttcatctcc tacgaggatt atctcattgc
    64621 agaggtcttc tttaattgca tggatagaac cttccacctc aggaaaaggt gctttatctg
    64681 tgccaaaagc atcaactgct atacgcactt cactccttaa tggcttttat ctgcctgcca
    64741 ttataagttc cacatttctc acacagatga tgaggacgaa ctggttcgcc acattttgca
    64801 caggtaacta ctgtcggttc agttaatacc ttatgagttc tacgtttatc tctacgggct
    64861 ttagaagttt ttttctttgg aactgccatt actttttcct tccttttaaa atcttagaac
    64921 agagtcttta tctggggaga tagataaaaa attattcctc tccccatttt tattcaggat
    64981 tatgtgcttt tgcatattat attttatttg tttagaacct catcttaact acataagttt
    65041 ttgtcaatta aaatagctat atctaattct ttaggaatta aatagaaccc ttttttcact
    65101 tactctttca accaatatcc atcaggattt tccacatttt tttctcacta tctcaacact
    65161 ggtgagttta ctctaatata gaaatagcat ttagttttac aatataaatc gtataagctc
    65221 ataaataata taattgagtt atgtcattta attatacttt ttcttcatac cagaataaac
    65281 tttttcccta acaaataaca gaggtataat gctcaaccat cttaaaattc ctcctatacc
    65341 aaacactacc tgttctggtt ctaaccatct attacccaat ttttggggtg caggaaagaa
    65401 aaccccggtc aaaagagtgg aacacatcat tgcaatacca cctaaagcac cgtaaatagc
    65461 actataagtc tgttctttac cttttcctgc gatagctaaa gagaaattgg tggtagcaat
    65521 agcatttcct gaccacataa atccagaact taaagcttca aaccatagca aagaaaagtt
    65581 ttgcggcgtt aaaaagagcc agagcatagg attaaaccca cctaaggcta cacagatact
    65641 cattgcggtc ttattgccat atctatcaat aaatttcccc cagaatgaat aggctaataa
    65701 agatgaaatg atgctaagtg tgttgtatgt ctgcaattga atcatactca tttgcagttt
    65761 ttttagcata aagggtcccc aaaaagcact acctacacca gtggctaaca tccaccagat
    65821 accaaaaaat agcaagctcc gaaaattctt atttctaaaa gcttcagaaa atttctctcg
    65881 taaagcccaa tcagattgta ccctgtactt tctatctggt tgcttattta aaatgaatag
    65941 actaatgata cctaaaatag tagcgataat gaatactaaa atgaggaaga aaggctgatt
    66001 ttgaggatta aaaataacca ctggattcgg gaaaaatctg gctaaaaggc ctccgttttt
    66061 ttcaaataaa tccgtgcaat aaccaaaaaa gtagccagct atcaatccag caaaagctaa
    66121 tatctgattc ctgcgagcaa aaaagcgacc tctaatagtt tgaggaatta aatcactgac
    66181 ccaagcaatc caaatattgc caccaatagc ttgtaatcct gcactaacaa agagtaatac
    66241 caggagatag ataattccca tccttctatc agaaaaaaac caggctgctc caatgaaaaa
    66301 acttagaaat ctgccaatac ccgtaaccca gatacagact ttctttcttt tctttaatct
    66361 atgggttaat gccaccccta aaggttgaaa tacagcagaa acctgtccga gggaacttag
    66421 tatactaaat tgcataggag tggcacctaa aagtaccata aatttagtga taaaactgct
    66481 tcctatatta gcaaggtttc cataaatctg agcaaaaata ccctctttga tggaaacgcg
    66541 ataggtgcgc gaaatcaaga atttcttctg ccgcattaaa ttctgttttt tagtgttttt
    66601 tttcctcttc cttcccaact ttcaaatccc ttaaatcttt ttcatttatt cgtttattta
    66661 cttcttgcta tttataaccc tatccttttt gcctgatata ttaatttagt attgaaaaaa
    66721 agcatccgtt tatttgcttt ttgccctgaa taaatgataa attatccctg attttgtaga
    66781 atagatgcga gatttaaatt gaatctattg aaaccgtttg ttttgcccta aaaaattata
    66841 ctttacactt attttttcaa ctttaaaatc ttatccattc ctgctttaaa ctttagccag
    66901 ctcttagttc tttcctggta tcaagtctta atcaagcgtt aatcaagtct ttatttatta
    66961 acgcttgatt aagaagtgat taacgcttga ctctatctct ggcaaaagat ggtgttatat
    67021 aaaggcgaag ataatatatt gtataataag aagattaact atgctctaaa gggaattttc
    67081 aaaggagcaa tagtgcgctc aaaataaccg tgcaatttgg atatagcgag tccataattg
    67141 gttataggaa ctcctcttcg ttctgcttcc cggattctat tattcatctc tttctgattg
    67201 agcatacagg caccacaatg tataatgagg gcataatctt ccaggttatc aggaaagtca
    67261 cttccggcag ctatatcaaa gtttaaatct ttttgagtat agtttttcaa ccaattaggg
    67321 attttcgttc taccaatatc atctttttga ggatgatggg aacaagcttc tgcaattaat
    67381 attcggtctc cgtcttttag ctcatcaatc ttctgacaac ctttaattag tgcttccaaa
    67441 tcccctttat atcttgcgaa aaggatagaa aaagttgtca aaggaacttg aggaggaagc
    67501 tgatttacca ctctctggat aacttgcgaa tcagtaataa ctaaatcagg ttcacttttt
    67561 aaagctttca aagccttgtt taattcaaat tctctcacca ctgttgttac tgctccacta
    67621 tccagcaaat ctcgtaaaac ttgcacttga ggaaggatca atcttccttt tggtgcagaa
    67681 gaatctatag gtgtaaccag aataactcta tctccagaat ggacgatatc tctgaccaat
    67741 ggtcgttctt cttttaagta gaaaggtgca agctcaataa ttttttcttt agctacagta
    67801 atattttctt ttgttgttgc tgaaacagaa atccaggcta aatcattgtc ctggcaaaat
    67861 tctatattat tttgatgggg ttttctgagg tctgatttat taaagataac gagaaccgga
    67921 agtttcatct cttttaatct ttgtaaagct ttcaattcat tttcctgaaa aggactacca
    67981 tcattaacaa agattgccat atctgtctta aaaagcacct tataggcggc tctaattctc
    68041 ttttctccca attctcccat atcatccaat cctgcggtgt catagaaagt cacaggaccc
    68101 aaaggaagca gttcatagcg tttagctaca ggatctgtag ttgttcctgg gacctcagaa
    68161 acaatagcta tttcctgatt tgttaaagaa tttatgagac tggatttacc ggaattgcga
    68221 tttcccagca aggttataat cagtctttct ccacgaggtg tactactcat ttttcctcaa
    68281 ccgtaattta tttctctttt agagactaat aaataaaatc aatcatttgt caagactcca
    68341 cttataagaa gtttactgca gattatcttt ttaaaaagta atatcttttt atgcttcata
    68401 acgatttaaa atgataaata gtaatgctct aagttctgat attgcaatat aaagcaaaat
    68461 ctctattgac attttataca tccatcagta aaggattaaa aaaatgaaaa aataatgaag
    68521 gtaaaagatg ccagggatta gtttaataaa atcaagagag ttaactaaca gtcacttaga
    68581 ctcaataaaa gaatttcaaa gcagtttaaa gcaaatacta cctcatattt cctgtgagat
    68641 actattccat aatgacgata ccataatcca tcagaccaca tacagaaact atgagttcta
    68701 ttatcagcaa agcgggaact ggttaataac ttttgaagga agaaaagatg atgttgattt
    68761 tggcagtatg gttgaatctt taaatctttt ttactcaaga gatatttcca cgaaagctat
    68821 gcaagaatgg cgagataaaa cttttaaact ttgtaaaaca gactttttct ttttagcctg
    68881 caatctggaa acaggaacag tggtttttgc taatgatgat ttagccagaa tgccagttta
    68941 tttctttcat cagaaaggca cttttatcat aagcagagaa tatccactgg tgaggaactt
    69001 aaataaggaa ttaataccgg ataggttatt attagcactt tatctgcttt ttaagtatat
    69061 tccaggtcag ggtactccat atgaagaaat ccgcacttta gataatagct caattggtgt
    69121 ttattatccc gcatcagata gtttaaccct gatttctgat atttccagaa gaatgcccct
    69181 ggaaagcttt aatggggata gaaaacaacg attaaatgat ttaaaagata gttttgtctc
    69241 tgccacagat gaatgtttag gtgatagaag aggactttta gctttttctg gaggtcacga
    69301 ttcccgttgt gtagctgcag ctttaaaatt aactcaaaaa ccctttatct ctgctacctt
    69361 tgttaatgat gatatttctt cccgaaatga tatgaaatgt gcaatgcaac tggctgaaat
    69421 attaaatatg gaacataagg tgctaaattt agcacctgaa gatgaaactc attataggag
    69481 tctttacact cttaaaaatg ggttaaactt tatgtctgtg gtttttatgc taaactttct
    69541 tcagaaactt aggaaagaat ttgatgaaga ctatgttttc tttactggag atggaggtga
    69601 tagaataatg caacctctat atccttctaa attaaagctt aaagaagata atatattacc
    69661 ttatctcttt gcaaaacagg ctttgtttga taaagagtat gttgctcatc tatttgatat
    69721 aaattcagct gatatagata attatctgat taatcttttg catagctacc cagtttctga
    69781 ctggactaat aaatacttat ttttcatatt agcagaaaat gcaggaaaat ggtcttttga
    69841 aggagaggat agaaatcgtt atttttatcc atctaaaacg ccattctata acttagattt
    69901 ttataaatta gctatatcca ttcctttaag ctggaaggac aatggagttt tctattggca
    69961 gtttatggag acattgaatc cagatatctt aaaagttcct attgctggaa ataaattgag
    70021 ccctgatact tggaactatc tactgttaaa gcgaatcaaa aactttctga gtaatttttc
    70081 ttttctcatt aacttaaaga aatttatagc aaaaccacaa cctacctcag tttccactat
    70141 taatcttgtt cagcaaagaa tagaaattct caatcaacat aagaacataa aagaatttat
    70201 tcccaatttt aatattaatg aattttataa taagaataaa gaatttaaca gagcgcattt
    70261 taccaatctt tatactgtcc ttacgatgat tacagaccag tgttagctat ttttaaaatc
    70321 tatttacctg tttatttttc tattgcctga aaactcagag ttaaaaaaga atatttctat
    70381 aatgatttgt atagagagga taaaagattt atgcagaaaa taggtttaat attctttttt
    70441 atagttcttt tctcattcct ggaagcagaa atcatccaag taggcaatgg aactttgact
    70501 aatcagagtt taccgattga agcttttcgt tcttatagct attctcaaca ggtttaccca
    70561 gccacactta tcaatagagc gggaaatata agttctatat cttttcaata caacatagtt
    70621 agtaatcatt ttttactaaa taatgaggaa tggaaagtct ggttaggtca cacttctcgt
    70681 caaaccttat ccacctttgt tccgatagat agcttaatcc tcgtttacga tggagtttta
    70741 actaatacaa acttttctgg aggtttacca ggtgagggtt ggttacaaat taattttacc
    70801 actccattct attataataa tgaagaaaac ttacttatag cagtggatga aaatagtcct
    70861 ggtggaagtt ccacttccga tgaatttcta tgcactgctg caccagtagt tcgtggtatt
    70921 gtctatacag atataaatat taatccagat ccagctaatc ctcctgttcc gcctatagaa
    70981 aactttttct ttgcacggaa tgcctatcct aatgtaaaat tggaatttac aagtacaggt
    71041 tatcttccca ctcagccagt tcctgctaat ggtgctacag gaatctctat aaacacaggt
    71101 ttatcctggc aaagttctgc cagctccttt gacctttatc tggggacaag tattgataat
    71161 ttgaccctgc tggcacaaaa cttaaacact acagaatggt atccttatga accctttcag
    71221 ctttggcaca cttattactg gcaagtcatt gctcatcaag gcgaaaacgc ctattctggt
    71281 cctatctggt cttttaccac agcaggactg ggaattggag cacctcaaaa tcttagagga
    71341 tactatattt ctggaagagt ggaattaaac tgggaacctc cccaaaatgg gaatcctatc
    71401 ctttatagga ttaacagaaa tgggaatttc tttgctgtgt cttataattt atcctatcag
    71461 gatacagaag tctgtgttgg acagacctat tactataatg ttagagcaga aaatgctatc
    71521 ggtgaaatat cggatccgtc caacacggtt actgtccata tccctgaaaa catacctcat
    71581 cttattttgg aagaaagttt tgaggaatgc tctcctttta gtggaattat cgctcaatgg
    71641 cagaatctgg atatagattt atcggaaacc tggaattggc aggaaataga ttttccaggt
    71701 gaaggacaag ctcatagctg gatttccttc ttccccttat tgactacacc cccattgact
    71761 ataatacaac ctcatagtgg ctcagcaatg ttagcatctt tagccagtgt caatcctccc
    71821 aacaatgatt ggctcatttc acctcaattt ttcttaggta acaatcccca acttaagttc
    71881 tgggctcgtt ctcatacttc tgactatggt ctggaaagat tgaaggtttt gatttctaac
    71941 actgatacca atctttctca tttccagcca cttcatacag gaaactggat ttcagtacca
    72001 gcagaatgga cagaatatac tcttgattta aggacctggg aaaatcaatt tgtctatctt
    72061 gcctttaatt gtgtatcctg ggatgcactg gcactttatc tggatgatat tatcattaca
    72121 ggtgaaggag gttatgttgc aataaatgat gaggttatat cttcttccca cttcttagtc
    72181 tatcctaatc cagctcgggg taattttact gtttccagtt ctgataaagt tctttttaac
    72241 atagagatat atgatatccg aggcaggaaa ttgttaagtg ttaagcaagt gactgaattt
    72301 gaaaagaata agtatcttcc agagcttgct gcgggaatct atcttataaa tctggaatca
    72361 gcaaaaggaa agttccagag aaaagtggtt attcttcctt gaaggataaa atgctctctt
    72421 ttgatataga agcttttatt tactcactac tgaccgatga gaaattcaga aatcagatag
    72481 ttgcacagca ggaacttgaa gcaagagatg ctatctgggc agattttcct tcgcaaagca
    72541 atccttattt actttcaatg cttagtaaac gagggataac taagctctat gctcatcaaa
    72601 aagaagctat tgaaacggca ttgaagggtg agaatgtggt tttaactacc ggtgtagcca
    72661 gcggtaaaag tctctgttat caatttccta tcctgcaaac tttgctaact tctcctaaaa
    72721 gtagagcctt gcttcttttc cctactaaag ccttagctca agaccagaag caaaaagtgc
    72781 aggaattaat tctgggatta ttaaaagaaa atcctcatat acctccaatt tacacaggaa
    72841 tatacgatgg agatactgaa gttggagtgc gaagaaatat ccgaaataaa tcccatctaa
    72901 ttttttccaa tcccgatatg ctacatctgg caattttacc caatcattca ctctggagta
    72961 acttctttgc caatttgaaa tggatagttc tggatgaagt ccatatttat aggggagtgt
    73021 tcggttcgca ttttgccaat gtaatccgtc gtttaaaaag gatttgcgaa ttttatggaa
    73081 gctctcctca atttatttgc acttctgcca ctgttgctaa tgccaaagaa ctaactgaaa
    73141 ccttactgga agaacctgtt catctggtag acaaagactc ttctcctcat agcaaaaggg
    73201 tattctttat tataaatcct cccatagtag attatgcttt aggaatcaga agaagcgctc
    73261 taatggaatc tactatatta gccaaaaatt ggttacagca taaagggcag gctatccttt
    73321 tttgtggttc cagaagaaat gtagagataa tctatcttta ccttactaaa gatgaaagtt
    73381 ataaagaaaa gataagaagc tatagaagtg gatacttagc agaacacaga agagaaatag
    73441 aaaaagattt aagggaaggg aaaatcaaac tggttatatc tactaatgcc ttagaactgg
    73501 gaattgatat aggtggtttg gatgctgtgt ttatgaatac ctatcctgga actatcgctg
    73561 ccactcgtca gcaagctgga agagcaggaa gaaaaaataa taccgcactt gctatcttga
    73621 ttgctggagc taatccttta gaccaatata tctgtcagca tccagaatat ctctttgaaa
    73681 acaatccaga aaatgcactt atctctcctg accattctga aatactacag agtcagctac
    73741 tatgcgccat ccaggacctt gctttgatgg aaaatgaagg ttttggaagt ctgggcagtg
    73801 aacatatttt cccctatctt caggttttac ttcaagaagg aaaaataaga aaagctaatg
    73861 atcgctatat ctgtgctcct gaaacctatc ccgcggcaga agtgtccctt cgcaatcttt
    73921 ccagccaagt ggagataatc tctaataatg aagttattgg aacagttgat tcttccagcg
    73981 caatgtggat ggttcatcca caagcaattt atctggataa aggtgacacc tggcaggtca
    74041 ctaaccttga cctggagcat aataaagcag aagtcaaacc agttcaagta aactatttta
    74101 ctcaggctac acgccaaacc tttatagaat gcaaaaacct tttaaaaaag gaaaattgtg
    74161 gaggctcaga taaatacttt ggacaggtga cagtaaccac aaccattact ggctttaaaa
    74221 agttaagatt ctttaatcag gaagttttag aatgggtaga actggattta cctccgacta
    74281 aactggaaac tattgcctgg tggatgggaa tttctccgca aattgtagat gcagtaaaag
    74341 aacagggctt atggaattca gagccaaatg actatggtaa aaactggcaa gagataacta
    74401 aagctatcaa aaaaagagat ggctatcgct gtcaacattg tggtgcactg gaaaaagaca
    74461 ttcctcatca cattcatcat atcattccct ttcgcaaatt tgacaatcca gaagaggcaa
    74521 ataacccttc taacttagta acattatgcc caaaatgcca tcgtttagct gaattaaccg
    74581 ttcagataca gaatggaatt accgctacag gctatctctt agtgaatctt gcacctttct
    74641 ttttgatgtg cgaccgtcag gacctggatg tattttgtga agataagtca gttcttactc
    74701 agaataatcc gattatactt atttacgata atatttcagg tggtattggt ctttctcgta
    74761 aactttatga cctccatacc caacttctgg aagctgcctt agatttggtg tctcaatgtc
    74821 cctgtaaaga aggttgtcct tcctgtgttg gacctgtggc agaaaatggg gttggcgcaa
    74881 aagcatattc tattgctctt ttgcaggaat taataaaaaa tctaccagat agataaactt
    74941 tcttactggg taatctggta gattaagata atttgtttaa tgttatcttt ttgctgcttt
    75001 agcttctttt tctttcttta tttgttgcct ctcttcccag agtttttctg ctacaggagc
    75061 ccatctttga gctgcaagta cggtcttaga acaaagctct tccactggtt ccggaccatc
    75121 cagttgagtg gaataagcat tggaattatt caccatttct atcaaaatct cgggattatc
    75181 taaaagagga cagggaatta aaggattgga attgaaaggt tgatgttttt tatattcctg
    75241 gaaaagaggt gcttgtaaag cttccagaag agatacatta tggatattaa cattggcata
    75301 atgaataaaa gcacagggtt caacatcgcc agcagcattg atatgaagat agcttctgcc
    75361 tccggcaata cagccatgag cagtttctcc atcattccag aaatccagag caattagagg
    75421 tttggttgtt cttatctctt gaactctttt atacatatag gctctttgtt ctggagtggc
    75481 tataaggtct aattgggcat ctttaccaat tggaatatag gtaaagttcc aagcaaaacg
    75541 acaaccccga gctatcattt catctacaaa ctcatcagaa gctacacttt cagtattata
    75601 atgagtgtag caagtggaat aaccaaagaa gagtttatgc ttcttcatca tatccatagc
    75661 ttctaccact cttttataac aaccctcacc tcttctcata tcagtgttcg cttcgtttcc
    75721 ttcaatagaa aaagccaaag tgaaattacc cactcttctg atatcttcac atagctgttc
    75781 atctactaaa gtgccattag taaaagcaaa gaaataacaa tcctgatact tttcacataa
    75841 gcgaatgata tccgttttac gaataagagg ttctcctcca gagaaaagga accaatagac
    75901 tcccagttct cttccttgct gacaaagaga atccaaaata tcataagaaa gattgtgctg
    75961 tttcccatat tgagctgccc agcaaccggt acaattaaga ttacaagcac tcgtggggtc
    76021 tattaacagt gccagaggaa cattgcaatt atacttttct tttatcttat ctcgtttagg
    76081 tccacccaca aaagcaacat taaccaggaa acattctgcc agtttttgaa gagttttagt
    76141 gtctacctcc ttaaataaat cctccgtaaa tttcttccag acatcactct cacccatcaa
    76201 agcatctgct ataatgtcca tcacatctga gtatttgtgt tttctatcaa gtttttggac
    76261 taaagatact atcttagaaa tctttttaac gggatcactg gtcaaatatt tcactaattg
    76321 atccaccgct atctgacgca tatttttcat aatcagaacc tctatttatc taatatataa
    76381 gaaaatacta tgttctaatt aggtttttaa agagcagcta aactgcttgc ttttttcttc
    76441 tattttataa tgtttccttt cctatatttt agttttataa aagaattatg caaacaaaat
    76501 aattaactta cattgccatc ttgacctatt aaaggcttat cctaagcaga gtttctttcc
    76561 cacattttca attattaaac taataaaaat agaaagagga agccgtcttt ctttatttta
    76621 attcaacgaa atagaagcat ttttttattg acaaaaaaag ataaaggttt attatgtatt
    76681 aagaataaaa cgctaaggag tcgtcatgaa aaaagattgt ttcttgcgca ttgcctattg
    76741 ccgggaagct ctttatgcca gatgttttaa tagcatctgg acggacgata aggaggtatt
    76801 gctacaactt tccgttttta gcgcaacatt gaaacctctg tggtcggaaa cttactccag
    76861 ctaagcgtct gataagtttc cgatgaagga tatttcccaa aatccgaaga cctcggattt
    76921 tccttttacc ttcaaaaaat aaaaactaca gaggtgaaaa aatgcaagca ttaggcagac
    76981 aaatattagc agaattctat gaatgcgatg agaatattct gcgtgatgaa gaagctattc
    77041 gtaaagcaat gttagaagct tgtgagatag gaaaagccac cgtggtaagt gatacattcc
    77101 attcatttag cccctatggt gtcagtggcg tggtggttat tgctgaatct catgtggctg
    77161 ttcatacctg gcctgaatat ggatatgctg cggtagatat ctttacctgc ggtcaaacta
    77221 tttctccttg ggacctcttt aagcacctac aaaaatgctt taaaagtaag cataccagta
    77281 atatggaatt gaggaggggc ctttttgata ccggaaatga accaattaag cataaacctt
    77341 attgaataat tatgagttac tggcatatag attatcacag taaatatcaa gctctgactt
    77401 ttgaggtaac agaggtcttg aaaagtatcc agagtcctta ccaaaagatt gaagtggtag
    77461 agactcccgc ttatgggaaa gtgctcttat tggatgatga tttgatgctg acagaaaagg
    77521 atgaatttgc ttatcacgaa atgctcgttc atcctacaat gttcactcat tcctatcccc
    77581 aaaaaatact tattatcggt ggaggtgatt gcggaacctt aacccgagtt ttacaacata
    77641 aaacggtaga aaaagtagta attgtagaac tggatgagat ggtcactcaa gtttctcaag
    77701 aatattttcc tcagttagtt tcctcaactc aggacccgag agtggaactt atcttcaccg
    77761 atggaattaa gtatgtgcat aattgcactg ataagtttga tgttatttta gtggattcta
    77821 ctgatccgat tggacctgca gaaggtcttt tccgcctatc tttcttccag gattgccata
    77881 aaatcctgga agaggatggc attttatgtc tccaatctga aagtccctgg attcctgaat
    77941 tggcagaact tatatcttat gttaatcaag atttaaaatc attatttccg gttgtgaaag
    78001 cttatggagc tgccattcaa acctatcaag caggtttctg gctcttccag ttggcttcaa
    78061 aaaaatataa tcccctttcc aaagaaatag aaaataaaat taaagaagct gatttaaaaa
    78121 ctaaatacta caatccaaat atacattacg gtgcctttaa actaccaact tttgttaatg
    78181 aacttctcca tgaaccatct taagataaca tagaatagtt ttataaaggt aagataacct
    78241 cttataactt aaagaaaata aatatcttat aagccagcag aaattattag cagtctacct
    78301 gtttgactgc taattttttc ttgctctttc tcttttttcg tttatcttgt ccttaaaaag
    78361 gagatgaata tgaacgcaca aagatttact ataaaaagtc aggatgccct tcaaggaatg
    78421 caacagatag cagaagagaa tgggcatcaa gagataaaag atttacattt acttgcggca
    78481 atgctaaaag aaacggaatc tttagtgatt ccggtattgc agaaactgga agtaaatact
    78541 acagatttgg aaagaaaagt agaagatgcc ttaaaaaaat taccgaaggt aagtggtggt
    78601 cagatttatc tttctcagga agttctggac atcttacatc aagctgaaag agaagcagac
    78661 cagttacagg acgattacat cagtctggag catattcttt tagctttatt agaaaaagga
    78721 aaagaggcag ccagattact tcaaaatgct ggggtaactc cagataaact acttttagct
    78781 ttaaaagaat taaggggtaa tcagcgtgtc actgaccaga atcctgaggc aaaatatcag
    78841 gcattagaaa aatatgctcg gaatctcacc cgacttgctc gtcaagataa gttagatcct
    78901 gtaataggaa gggatgagga gattcgtcgt accattcaaa ttctttctcg ccgaagaaag
    78961 aataatccta tccttatagg tgaacctgga gtaggtaaga ccgctattgt agaaggattg
    79021 gcaaaacgaa tagtaaatgg tgatgtacca gagaacttga aagataaaga gattctggaa
    79081 ctggatatgg cggctctttt agctggagca aagtttcggg gtgagtttga agagcgattg
    79141 aaagcagtac tttcagaagt agaaaagtct gaaggtcgta ttatcctctt tattgacgag
    79201 attcataccg ttgtaggagc aggtgcagca gagggagctg tagatgcttc caatatgctc
    79261 aaacctgcat tagcaagagg aactttacat tgtataggtg ctactacaat tgatgaatat
    79321 cgtaaacata tagaaaaaga ccccgcactg gaaagacgct tccagcctgt gatggttcag
    79381 gaacccagtg tggaagatac tatttctatt cttcgcggaa ttcgggagaa atatgagatt
    79441 catcatggtg tacaaattac cgatactgca cttgttgctg ctgcagtgct aagtgaccgt
    79501 tatatctccg atagatttct ccctgataaa gccatagacc tcatagatga agcctgtgcc
    79561 aaactgcgga tggaaatgga ttctatgccc agtgaattgg atgaagtgga gcgtaaactt
    79621 cgtcagctgg aaatagaaaa atattctctc gccaaagaag aagacaagct ctcaaaacag
    79681 cgtctggaaa agattaatga agaaattgca gaactaactg aaaaacgtaa taccttgcgt
    79741 ctgagatggg aaaatgagaa aaagttattt gaacgaactc gtgctttaag agcagaaatt
    79801 gaatccttaa aattacaggc agataaagca gaacgtgaag gtgattacga aaccacagca
    79861 aagttgcgtt atggcatcat taaagaaaag gaaaaagaat tagcggagct tattaatcaa
    79921 cgagagaatg cctctggtga ctatgagccc ttaattaaag aaaaggtgga tgaggaactc
    79981 atcgctgaaa ttgtctctaa gtggactaat ataccggtgt ccaaattgat acaaagtgag
    80041 atggaaaagc tgctccatct ggaagaaatc ctttctcagc gagtagttgg acaaaaagaa
    80101 ggcatcactg ctctggcaaa tgctattcgt cgttccagaa gtggtttaag tgataccaat
    80161 cgtcctattg gttccttcct gttcttagga ccaacaggag taggtaagac tgagctggca
    80221 aaagctctag cagcttacct ctttgatacc gaaaaggcta tgataagact ggatatgagt
    80281 gaatttatgg aaaagcactc tgtttcacgt cttattggtg ctcctcctgg ttatgtgggt
    80341 tatgaagaag gaggttattt aaccgaagga gtacggagga gaccttattc ggttatccta
    80401 tttgatgaaa tagaaaaagc tcatcccgat gtgttcaata ttcttttaca gattctggat
    80461 gatggaagat taactgatgg aaaaggaaga acggtagatt tccgtcatac catcatcata
    80521 atgacttcca atattgcctc tcaagagatt tacgaggcag aggacctgga agcattacga
    80581 cctcatttga tgagtattct acaacaatac ttccgtcctg aattcttgaa ccgtctggat
    80641 gatattatta tcttccatcg tcttagccgt gaacatataa gagatattgt aaatatccag
    80701 ctcaatcaat tggcagaaag aatcaaagaa aaaggtatag aacttagctg gacagatgcc
    80761 ctagtagatt atctggctac tgctggatat gatcctcaat atggtgcacg tcctttgaga
    80821 agattgattc aaaaagaggt tgaggaccta atggcaaaag ctataattgc tggtcaagtt
    80881 ggacctcaaa tcaagctgga ttatcagaat ggttctgtag tcgttagcta aaattgataa
    80941 agttatagcc gcttccacag ttaggaagcg gcttttcttt ttatataaat tccagattat
    81001 aaatatctca atagttcacg gtctatcaca ccagcataaa gttctatata cggtttgact
    81061 ggactattat taatcttggg gaaaaagacc tcttctactt gaaagaaacc caccgaagca
    81121 ttcatttcca ccgtaatagc aataggtttt tcttcccctt tatgaatccg aactctttga
    81181 atagcgctgg cagaagtgcg atgaatatct cctacgcggg gttcactgga aagcatagca
    81241 ggaatccttg ctctaccttt ttccatatca cttccatctc caatcaatac cagccctgct
    81301 tccagagaat ggatatgttt tgttgccata tgaccaaaga ttccctcaat agcggttgct
    81361 ttgatagctg ttttggtcat cagctcttca ggattgtata tctcatttaa aatgcggtct
    81421 ataaaaggaa tagctaattg aatggagaga atttcatggg aatcacgggc aatagacata
    81481 ccaaggtcgt gcagtaaaga ggataaaaga acagcagtta gagaatcttc tgcttttcca
    81541 actccttcct tttccagatt catcttgatg cctgcttcat tgagtaattt gaacatctta
    81601 atagcattta aagtggcttt acgcatatga actggtccat ggtcattata ccccaatctt
    81661 ctgatagcta caatattggc atattcttgc atagtctgaa gttcagggtc attaaaaagc
    81721 atttgcgctg cttgtaatgg cttaccctct aacattttca atattcttgt ttccagatta
    81781 gtttgtttaa ctgatttata catacaattt accttaaaac ctgtttttca tactaaaaat
    81841 aagattggtt atgctactaa ctactgctat agctaaggca atagttccag aaatcaatag
    81901 cactttataa agattttgag aagcaactac actatctcca gctatataag cttgcatagc
    81961 ataataaagg ctacttccag gaacaatagg aataatgacg caggtgacaa acacggaaac
    82021 cggtatctta cttaatcttg ccattatctc tgaataggta caaacaagaa acacagagca
    82081 aaagactgaa aatagtaatg acttgctgaa atagaggaat atgagataaa aagcccaggt
    82141 taatcctcca ccaattgcaa ttaacaggat tttccagccc ttaagattta ctcttatgga
    82201 gaatccgaga atggaaagag ctgcccaaca gaattgcatt aaggtcaaat gatcaaaagg
    82261 gcgcattttc acatacccca cagttcaaag agttttagca taataccact tccagcagca
    82321 atagcaatgg ctaaaaatat tgcttccaca cctcttgcag ttccagatac cagatctcca
    82381 ctcatagtgt cacgaatggc attaacaaag cttaatcccg ggactaagag cataattcca
    82441 ccaataatta tgttatccaa ttttatatag ggaacccata tatccagcac tttagcaaaa
    82501 acaacgatta aagcagctcc cagaatatgg aggagaaagt tatttaattt taaattcccc
    82561 agaagtttta atgataaact taccagcaca ccaacagcat aagcaacagc aaattccagc
    82621 cagctacctc caaataataa gcagaaacat ccactggtta aaccgccaca aagaatttta
    82681 aaccagtcag gccaagcttt ttcattttct atatgtttta gctcttccct aaatttatcc
    82741 gcattccagt agcaaactcc ttctttacag tattgccttt ccatatcgta taccagatga
    82801 ctaattttgg ttattttccc cagatctgtt tgtctgtttt ctattctttt tatactggta
    82861 gtaaaaattt ctttaccatc tgaaacggta agaaaaatcc ctgttggtgt aacataggct
    82921 tcggtttcag gatatccaaa aatactgcag actttgcaca tcataagttc cactctatta
    82981 gccgtagaac cattctgcag caaaatcctg cctatttcca tactaatttc taaagcttcc
    83041 gtgctggtta tgcgattcat ttttctttaa tctcagtata ttttaaaact tcttccggca
    83101 ttccaaggat tcttgctaca tttatggcat ttagaggtgg agcttcattc tttttcaaac
    83161 gcttaatgct gtaatccata gcttcattaa gaatttttag tctctcgttt gcatccttag
    83221 gattttgagc tttaatttta tccaaatcca atcctgctat actatattga gtaaccttgg
    83281 gaagcattgt tggtgcggta aaatgcgttg cggcgatact catatttttt gtagtggcaa
    83341 gatattgcaa tactgcagag accaaaagct caccttcagt aggattagta ccacgagcaa
    83401 attcatctaa aagaaagaga tttgtcccag actgctgtat agcctgagtg aaagatacca
    83461 cttcccttcc aaaactactt aaatcagcgt tattatctgt ctctgattga ttataccaga
    83521 tgtgcttaaa tatgggtaat tctgcagatt cgcagggaag aggaatacca tagtgaagca
    83581 aagtgcaaaa ctgaccaatg gtttttaaaa ccgtagtttt ccctcccata ttaggaccag
    83641 ttagaagaga gacagaaccg ctaaattcat aatctatagg ttgatatttt ctgcccatac
    83701 tttccagatg ttgttttaga ggaagattaa ttgctttctt aattataatt gctttcttcc
    83761 gccaaacaac tggtatacaa cagttatact taaaagcaaa gttgctcaac agaattttcc
    83821 atgcacccct tcttaaagat aaccagctta aaaagatatc gctttcccat tccctgtaaa
    83881 tatcagtcag ctcttgtaat acatagcgtt cttctgcttc taatttttcc tggagattac
    83941 ttattttctt tttaagttcc agacaatgtt catcatccgc taaagcaaaa ctataatttg
    84001 ccacattttc agcagttaga acaaaatagg gagagtgaat tatgcggtca gccaattccg
    84061 tttcacttcg cgaaacggta aaactatctt taagataggg aatttgcaat tcctttttgg
    84121 ctctttccag aagctgatta taagattctt ttagttcttg caacaatttc aattgcttat
    84181 ttaaaatatc tcctatttca tcagaataag cagaggaaat aacaaatata ggtaacccaa
    84241 atccttcagg gtcaagtatc tcaaaaaatt cggtaagatc ctttaaatta tcaaagagat
    84301 ccagccactt ttgcttttcc aaaaaatagt ataattgatt atagtaataa acatattctt
    84361 ttatttcata taattgatgg acggttaaaa ttttaatatt tttatgtatg ggaaactcta
    84421 aattaggaat agccgccaag agttccagca aggtcttcaa ttttgattta tctgcagaga
    84481 gctgttccat agtttgttct aaatatttat aatgtatggg taagtaatag ttatcgtata
    84541 aatcttcaga atatccggtt aaattatcct tcttaactga tttaaggaat tcaccttccg
    84601 cttcaatcat atccagtatc tgagggacac ccaaaacttc ttccatctga gtaagcataa
    84661 aattcattct cctctctaag ttattaaatt actttatgtt ttgatttcac tcagctgtca
    84721 atctttttgt gtaaatggat gatagatagt tccatcagca gtgctaagaa ttctctgggc
    84781 ataaagctgg aaattgtgca tcggttctgg atgagggaaa ttccattgat ttctacgact
    84841 aacagaaatc caaacttctt cgggaagaac agctctgata aattctctac agctggaact
    84901 tttactccca tgatgagccg cttttaaata gtcagcctta agttcttctg gataatgttc
    84961 cagcatataa tattctgcat tgatatcagc atctccagta aaaagatatc gcttaccctg
    85021 gtaatccaat cttactatca gggaagaact attggaagtg gcaggataat agtatttagc
    85081 aggatgtaaa aacttcagtt ttgcctctcc aattctgtaa ctgatggtat cttcaatgca
    85141 atgaatggta gccttattca aaagaacaga tttttccaat tcgctccatc gttcatctga
    85201 catagtttca tcggttacag caatattctt tacttttaaa ctgcttatta tatcaggaaa
    85261 accaccagaa tggtcagaat ccaaatgagt taaaattagc caatctatct cttttatatt
    85321 tttatgattc agccagggta gcaacttttg tgaagcccag ctttgatctg aagttctgta
    85381 gtgtggtccc gtatcaatta taatcttttg tccttcatta agagagacta agatacaatc
    85441 tccagtgcca caattgaaaa gatagatacc tctatctgct gaatgatgaa attgaggaat
    85501 aatgattaat gctataccaa ataaacagat aacaagtttt tgccagcgta tttccagttt
    85561 tttgagcata gaaagtccac caataactaa aagtatacat ccagctaatt gtaatcctgt
    85621 aatccagcga ttttgtagat agaaaggcat actggcaaca aactcagtcc accgattaaa
    85681 gatacaaagg aaaaaacgat aggaattaat cagggaagca gaaattatat ttcctgccgg
    85741 aatcattaga ataagaaaac ttaatattaa aataactcca gaaagaggaa ctcctaatat
    85801 attaccaatt ataccattta gcgaagcagt gccaaaataa aaaagcgtta aaggcattac
    85861 cgacagacca acaaccagat tcattagcaa taaatcaaag aatccattca ttttattgcg
    85921 tatatttgat gctggtaaat ccttttcttt tatccattca atgtgcggta gaccaagtat
    85981 tatcacgccc acacaaagaa aagaaagttg caatcctata tcaaacaatt gctcaggatt
    86041 tatagcagta attattaaca aacttaatgc cagaagttgt gcaccaccaa gaggaatatt
    86101 tctccagcgt gcaattatca tcaagcctat catcaaaata gcacgtaaaa ctggtggtgc
    86161 ccagtaattt aatgcagcgt aaaagattat caaaatgaga aagacagttt ccgcaatttt
    86221 tggtggaaaa aaagcacgca aaaatatcat acatactgca taaataaacc agatatgcaa
    86281 tccactaact actatcaaat gaatcattcc acttctggtt aaatcatctc ggtatttacc
    86341 tttagcagaa gtatccgaaa aaagtaatgc tttagcgaac tcagcttcat ctcccaattt
    86401 attttccaga ttggcaagca attttgtccg ccataatgct atggggaata tagacttagt
    86461 tgttttttcc aattttaaac tttgtctgat ataagcacga ggaagagagg aatacatatc
    86521 taaaacatca tcactttgtg ctggaatgat ttccaataat gctgaatatt cctgtcctgg
    86581 ggttaattta taatcgctgt ataaaagtag cttttccctt accctaattc cagcaacttc
    86641 atctaatctt atcttataga gattagtttt ggatgatatt aaattggtaa ctaaaaatct
    86701 tgaatcttgc tggatatgag acttggtttt aaatacttct tctaaaacag aaggttgctg
    86761 agcaattgtc catcgcattc cacctaaaag cagacaaatt ccaagtatca gatatatacg
    86821 cgcagatttt aaaaaaatag ccaggataaa aattaccaga gctataattg cttgccagat
    86881 aaagggaata gctattgccc gggataaagc aatacctaca caccacaaaa gaacaggaag
    86941 gaatagaggt gcaggttttt catgctgaag catttctatt taatcccaaa aacaattgta
    87001 aaataaccta tcaaaaaatc catttcagaa ctgcacctaa aaagttatct ctctgtatgt
    87061 taaagaaata aaacatttac ttattctcat agatgttctt tctctttcct aaagtttgat
    87121 aattttaatt taattttatt tcttattata atctgtcaat aaaatttacg ctcggtatca
    87181 taaatccaat taggaaaatt gcctcattat gtcttaacaa tcatatttga aataataaat
    87241 tttcatatct attttgatag tacactaaat atattattac taaagattta ctacatcctc
    87301 acttttatta gctaattaaa atctacctta tgggtaactt atgtccactt tcggacatga
    87361 gctggacatg agttggacat aagctggaca taaactctac ataagctgga catttattat
    87421 ttatttagag ggagtaatta cataatttgc agtggaatat aaagaataga gt
//
LOCUS       DAORZF010000003        78495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_263_length_78495_cov_369.629271, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000003 DAORZF010000000
VERSION     DAORZF010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 78495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 78495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78495
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_263_length_78495_cov_369.629271"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PK015_00855"
     CDS             complement(<1..296)
                     /locus_tag="PK015_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33054.1"
                     /translation="MTIVLLCSMAFAWDLIRQTTIPTNFYCLEKIGNTYWAGGYVGAV
                     AKSTDNGLTWRFVETPAYNASANNYKDVWDIDFINEMQGILVGDDGLVALTNDGG"
     gene            615..1718
                     /locus_tag="PK015_00860"
     CDS             615..1718
                     /locus_tag="PK015_00860"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOL33055.1"
                     /translation="MENRTWEYLSVFIMSFLFVYGLTPFIIKLARALNFEDKPAARKI
                     HQQKTPLLGGLSVFIGFSLLCIYDVAISPNRYFDLPMIGYLLGGLLITVLGLIDDKFG
                     MHPAIKLLGQITVSLIFILSSFRIAELNHMFGSIYISLPLMVLWMVGLMNAMNFLDNM
                     DGILSGMAGILGLGYFAFSLANITGSNSSEMAFIGLISLSFAGATLGFLPYNFNPAKI
                     FLGDAGSMFIGYFLSSMGILMGRFAVLTRQNNIFYLLPVLLLSYAIFDICLVSYTRTR
                     DGRHISQGGRDHSTHRLLTMLGSVKITAIIVYALNLMIVLTTIIIFITGNGVLLIATA
                     IMFTLFFLFLGHKLDQIPIVIPINQQKTGRSLK"
     gene            1715..2452
                     /locus_tag="PK015_00865"
     CDS             1715..2452
                     /locus_tag="PK015_00865"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HOL33056.1"
                     /translation="MKFILLGTGSGMPELGKNQSSLYVNVNGKHLLFDCGEGVSQQLL
                     KHHLDNNVLDAIFISHYHPDHISGLFMLLQMLKIQKREKPLQLFLPERPAALVETLQF
                     LYTFIQKFTFPLHILDCEESELYYEEVNVALTDHLLEYEDLVNESKLPNLLHSYAFRI
                     SGPEGSLAYTSDINKVESVQFLIRNCHTVIIDAQHPEADQIIRLQYSGIKRVLLTHGI
                     SPTLQAFLFENPIDMFEFAQEDITYII"
     gene            2449..3858
                     /locus_tag="PK015_00870"
     CDS             2449..3858
                     /locus_tag="PK015_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33057.1"
                     /translation="MKYKYIIFILLSLVLLVISACEINQLNSAEDHYKNKRYAAAIQE
                     LDNYIQTGKNGALITRGEILRSQCYYELGLRAIELENWDLSIKFLKLANSEEADKVLA
                     DVYKKLADKALEEGELELSFNLVNTILREIPHSELTAEMLSRRISFLLDTFIDYEQAW
                     QDYMTLYNNYPNNVYEVAARKQIDRIIPSRINYAQRLYSSGYYSDALNILFELQKYPV
                     GDPQAINKMICEAYMGQAENSLQQQNYLEAERLFRIAIQYDPSRKVEAEKRIEQMASL
                     FVQKGDELVRQRDYDNALIHYQKAFDIIPDYPPAKQAIARLNTIKENVARANELYAQA
                     EKAELAGKYSEALKLYRQANSLDSRPELTTKISQMQNLIEAQADPIAFTKRIINQYKG
                     GIINTRIQKQKQELLKMFDPSEVQDSGWKIMITSNPLKYEARYDLITPTVSYYYVWLV
                     NLRDGSLTPLNKLSEKTME"
     gene            3861..4445
                     /locus_tag="PK015_00875"
     CDS             3861..4445
                     /locus_tag="PK015_00875"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HOL33058.1"
                     /translation="MRKFMLISLLLFLTVMLTAQTQFDINVFADTTKYGWQDWRDRLR
                     YREDLMHRQQLLQIYEMQSNPLRPTVLKSVVIPGWGQLACKDNLKGSVILGTEVLSLG
                     VSLYFYDKSNYYYNKYMQANQIEQIEDYFDKAQDQRFYSLLFMGLGGVIWAYNIFDVI
                     QTTEDYNAQVWQEILEKYSQKTVTLTPGGIRLQF"
     gene            4455..4892
                     /locus_tag="PK015_00880"
     CDS             4455..4892
                     /locus_tag="PK015_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33059.1"
                     /translation="MKFKIILLIVITLFIGSCLQRNNPLDPVGNPNVIVPDEVRDVSC
                     TASPPGATHKYVIIRWTANNPENTDGYKVYRGLAYSANYTCVDTVYTNECNHGSKAWH
                     TVLPGDYWYRVSGYKDIYDINTHQYIGRLEGRLSEARFVRVPL"
     gene            4903..7089
                     /locus_tag="PK015_00885"
     CDS             4903..7089
                     /locus_tag="PK015_00885"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOL33060.1"
                     /translation="MHTDFKQELNEQQLQVVLETERPLLVLAGAGSGKTRSLIYRCAW
                     LIKEKKVPPKNILMVTFTNKAAKELQERLQNILGYSVQNLWVGTFHHICTRILRNEAS
                     FLPFRQNFSIYDENNQLSLLKKIYKEHSLDRERFPLQPVLNRISSYKNRLLLPEDLNE
                     YNLEISMENYKLDRTVPKIYELYQQNLLMNNALDFDDLLLYTAKLLQNNELVRKKYQQ
                     QFQYVMIDEYQDTNYAQFEIVHQIAAEHQRICVVGDDDQAIYSFRGATIRNILEFEKD
                     YADAIAIRLEQNYRSTSHILELANAIILNNRHRHSKTLWSELGEGYKPELFVYEDDME
                     EANKTADLILQLHEEGIPFREIAILYRTNAQSRVFEYACLQRNIPYTIVGTVHFYQRK
                     EIRDLLAYLTVIFNPSDNNSLLRIINEPPRGIGSVSINRLMEFASQNHLSLYQAIQNV
                     QAIQSIQPGIKKKIAEFAEKLEDWHRKAENTPVLDLVKEVIDSLDLINHYKNSQDPAE
                     ISRVENLIEFVSSVSEFSERWIEEYDKSPLLEDFLPFVALQTDIDRVNEAADTVYLMT
                     LHNAKGLEFECVFITGLEQELLPHLRSMNSKYSIEEERRLLYVGVTRAKSRLFLSYAK
                     YRRKYDSFYFTEPSIFLQELDPNLFAGYNDAVTFKPKPANKVYAKPKTLNNNQFYIGQ
                     KVWHTEYGPGLVLNVDGNGPEARLTISFSSGKLAKIIASYVSPEPI"
     gene            7089..10490
                     /gene="mfd"
                     /locus_tag="PK015_00890"
     CDS             7089..10490
                     /gene="mfd"
                     /locus_tag="PK015_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOL33061.1"
                     /translation="MTPMLYEVILPYLNASPFFQKLSKLFGTKQHYQIHHLNQSARAL
                     VTAHLWKETGKNILIVSQDDLMAEDLWDDLCTLIGQENALYLPEYEVLPYEERSPHYS
                     IRATRMMCIHHILQGAPSVYSISIRSLLRMLPPLENIAKHIKELRPQMEYSPDKLMQD
                     LVWMGYDVQYQVTKVFQVARRGGIIDIFSPPQLKPVRIEFFGDEIVSMRYFSPNTQRS
                     IPGDVESYTLLPSREFSLDDISPHSILLPQIKNTGFYEGIENQYCLLLDKLSTFADYF
                     EAQNRLILFSNFPYIQEELTRLTEQVQLEYHRQLKYTTKAKLSPPEGIIAGEEKLMEL
                     CQPESSLFLSQSEFILPFPTENIKAPIEPQPSFNGNLTLLASALNQMEKEHFNPILLF
                     DNISQSKRMQELLEDYNCSPQSYIGVLHEGFTITDAGLALWTDHEIFNRYKRKRYTPR
                     FPPGEEIVDYQSLKPGDYIVHIDHGIGVFEGLQIIKLDGQDTECLVLRYAGDDRVYVP
                     TYQLDLVTKYIAEEGVAPTLSRLGSTKWQHTKRKAAEQIELIAADLVKLYAERSSRIG
                     IAHQTDTPWQKELEESFIYEETEDQLRSIEEIKADMESPVPMERLLCGDVGFGKTEVA
                     IRAAFKAVTSGYQVAVLVPTTILADQHFRVFKERLAQYPVRIAMFSRFSSNSQIAEDL
                     ADLALGKVDIAIGTHRLLSRDIHFKNLGLLIIDEEHRFGVRHKERLRKLQSNVDTLYM
                     SATPIPRTMNMALAKLKEISLMQTSPKERLPIRTIITLREDEVIREAIQREIDRGGQV
                     FFIHNRIQTIETVAKQLKELMPGVKFAVTHAQMPEKQLEDIMGAFINREYQVLISTTI
                     IENGIDIPNANTIIIDRADTFGLAQLYQMRGRVGRSNRRAYAYLLISRKTTPEARKRL
                     ETLTQYDYLGAGFQVALRDLELRGAGTILGTKQSGIIQSIGFNFYNNMLENAIAAVQK
                     GESIAEQIETPKPQRKLFTEIDLYFPPDYISDDEQRLTIYRRLSELDSLQDIEDLELE
                     LKDRFGPLPEKASWLLMYFKLNILAKQADLQNCYVRHNVLTMEFKLENLPPRDKVLSF
                     TAKAPGVLRIDASKGLKITISLDEKKKYQDQFADALELLQLYINS"
     gene            complement(10493..13831)
                     /locus_tag="PK015_00895"
     CDS             complement(10493..13831)
                     /locus_tag="PK015_00895"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOL33062.1"
                     /translation="MIEVILNGKKIESEAGITILELAKRNGIKIPTLCDDEELNPYGS
                     CWVCLVEVKGRKGFVTSCGTTIAPGMEIITDSAEISNARKMALELLISNHYADCEAPC
                     KLACPALVDVQSYVSLIANGQYHDAVKVIKKTLPMPLSIGRICPAFCEKECRRQIVDE
                     SIAIRQLKRFAADEDLNDVWSYVPEKKESTGKKVAIIGAGPSGLTCGYYLSNQGYEVK
                     VFESAAEAGGWLRYGIPEYRLPKDILDREIELMCSNGMEIEYNIKLGQDLKLNELSQA
                     YDAVYLAIGAQKAVPFPVKGSDLQGCFLGVDFLKAHSLGQTPYLGKKVAVVGGGNTAI
                     DCARTAIRLGCEVTVIYRRTRQEMPAEPEEIEAAEKEGVIFHYLSNPVEYIGSDGKLQ
                     QVKIEKMQLGEPDSSGRRRPVPTGEYFLESYDSIIAAISQIPEVEIFAQEDNFIQGQI
                     LPISRWQTAIVDEKTMYTGLANVFAGGDFRRGPATAIEAIADGYFAAEAIDRFLKGQK
                     IEKDTFRFDSKKGYKIQDISPKEYRKYEKIAREKMPNIPIDKAKNSFEEVELGFNEIS
                     AKFEAERCLECGCQVNETCKLREYCTDLKVEALSYPGSINKYCIDYSHPYILRDPNKC
                     IDCGRCVRICSEIQGPGVLGYIYRGFSTLIAPEFGESLTKTSCESCGKCIAVCPVGAL
                     VERNINYKLNPLEKEKTKQNCGICGTGCCIQCESQSGVPVLIGTDEKAPGFNGRNLCF
                     KGRFGWQAFYTEERLKSPVVKRKGKWEPINWQETYSLLQEKVKELSSYAFAISPLITL
                     EEMLILGKAAKNTGSVLYASQDYHFFSSQYLPLKPNLEPYKILEDFSEFVVIGEISQN
                     LRTLLRLQQKKGKRLVSVLCQNSTALHFADKVYSSLSALQGNKNQLFIYNINQISEKD
                     IYEILNLAVSLDSGLGNILESSDYPNYYGLQLLNSEWKDNVDAELILSYGTKSKKSSN
                     TRFSVEILPFIDSSSEADLILPQPTYLEIDGTALANQGYITHYYNPAKSNLINQILHL
                     FLQLGWIPPAGGDMNYWNQLAEDSIKAGFNHQLYSYQPEKIEQKDLRDYVVSANDIGN
                     QKINIFYEKRKEPSSFERRLYKNR"
     gene            complement(13841..15610)
                     /locus_tag="PK015_00900"
     CDS             complement(13841..15610)
                     /locus_tag="PK015_00900"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOL33063.1"
                     /translation="MSEIIVKVGLASCGRAAGAMEVYDKLKEYLEQNEGAYILKKTGC
                     IGMCFEEPIVSLSGSELGEITLGKVNAENVINLIEEYRKDIIPSSNIILSDKVEAPCN
                     DLFAYQQRIVLQNCGRIDPLSIEDYEASGGYQALRKALTLSPQEIINEVKISGLRGRG
                     GAGFPTGLKWSFAAAAKSDIKYIICNADEGDPGAFMDRSVLEGDPHRVLEGMIIGAYA
                     IGAHKGYIYCRAEYPLAIEHLKMAIVSAREKNYLGDNILGTDFSFELSIKEGAGAFVC
                     GEETALMQSIEGKRGMPSIRPPYPAESGLWGKPTNINNVETWANIPWIIKNGGEAFKA
                     IGMEKSSGTKVFALAGKIAGSGLIEVPMGITLRDIIYKVGGGMKTEKPFKAVQLGGPS
                     GGCIPADLLDIPVDYDSITATGAIMGSGGMVVMDSGTCMVDVARYFLNFTQNESCGKC
                     TFCRIGTRRMLEILNRITEGKGELEDLDRLEELALNISKGSLCGLGQTAPNPVLTTLR
                     YFKDEYLAHIVDKRCPAGVCTALLHYYILPEKCIGCTLCARMCPVSCISGSLRQVHII
                     DQSRCTHCGNCYKACKFGAVTRE"
     gene            complement(15621..16085)
                     /gene="nuoE"
                     /locus_tag="PK015_00905"
     CDS             complement(15621..16085)
                     /gene="nuoE"
                     /locus_tag="PK015_00905"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOL33064.1"
                     /translation="MEQDFISVDEILQEYEGKKGNLIPILQAVQRQKGYLSKSDIRYI
                     SEKMHIPIAEIYGVVTFYAMFRLEPQGEHIIKICKGTACHVADADSIKEAVVDYLKLE
                     EGEQTTADGKFTVMEVACLGCCSLSPVIMIDQQTFGKLTPNAIPGILSRFKK"
     gene            16680..17483
                     /gene="mutM"
                     /locus_tag="PK015_00910"
     CDS             16680..17483
                     /gene="mutM"
                     /locus_tag="PK015_00910"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HOL33065.1"
                     /translation="MPELPEVQTILNELEKELKDKQIEGLDCYHSRTLLWDKELTATP
                     FPAKIIGLERRGKFILIHLTNENTIIIHLGMTGKLVRDNTIANHSEHERACFRLSEKE
                     KIHFIDIRTFGKIVLCPTKNIPAYLPELGVEPLSEDFNLSYLQKALKGRKAPIKNILM
                     DQKIIAGLGNIYANEILYRAKINPETPASSLSSQKLSKLIKTTKTVLNEAIEKNGTSI
                     SDYRRIDDKKGEFQNFLQIYQKTTCPKGHPVQKIKQGGRSTYFCPVCQK"
     gene            17574..18755
                     /locus_tag="PK015_00915"
     CDS             17574..18755
                     /locus_tag="PK015_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOL33066.1"
                     /translation="MKAYKLKDNIYWVGAIDWNLRNFHGYITPRGSTYNAYLIIDEKV
                     TLIDNVKYYLFDEMLARISDIIDPHKIDIIVQNHIEMDHSSGLPQLMKIIPEAKIYTN
                     ASAIKGLKLHYHQDWNFQEIKSGDSINIGKNDLTFLTTPMVHWPDNQVTYCPQEKLLF
                     SNDAFGQHIASNERFESDFPLDIVLEEAKKYYANIVLPYSRQVQKVMEAVSSLEINMI
                     APSHGLIWTEHIPEILNAYNLWSNNITDNQKALVIYDTMWKSTEIIAYAIAQAFENKG
                     IKAKIRNLQLTHISDIMTEVMDAKYICVGSPTINSSILPTVAAFMSYLQCLAPKERIG
                     LAFGSYGWSGQSVPMLHKMLGDDSGCGFKMLEPVKVQFIPDDEILNQIRENLERQLDN
                     M"
     gene            18897..20015
                     /locus_tag="PK015_00920"
     CDS             18897..20015
                     /locus_tag="PK015_00920"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOL33067.1"
                     /translation="MKKESKKKIIGLFDGIDDIIVNDIKQQLRTVKFKKGDKILREKE
                     EGSCLYVILKGKIEIEKEVEHSEPPTAPLTVMEEGEFFGEMSLLNEEPRSANAIALED
                     TELLEIPEEEFQRLCFSYPNIMLNLVRALSERLRLTNERFAGALSEMIQKNRLTAIGK
                     ATSKIIHDIKSPLTIISLNAQLIETLFPEAEELTQGIIKQAKLIDELLQETLDYIKGN
                     PNHLIIQKVDMVKFLKDIEDTYGPSLKSRNIELVIENKCNEPVYFDEERIRRTIINLI
                     RNSSEAISTKGTIKIIANLSSNWLQISVIDDGPGVPPAIVDDLFKPFISYNKPTGTGL
                     GLSICQKLVQEHKGRIEYTPVQPHGARFDIRLPQQHNL"
     gene            20147..20338
                     /gene="rpmB"
                     /locus_tag="PK015_00925"
     CDS             20147..20338
                     /gene="rpmB"
                     /locus_tag="PK015_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOL33068.1"
                     /translation="MSKVCDICGKKAQVGNLRSHALNATKRRFYPNLHLVRAVVDGEV
                     KKIRVCSSCLKTNKVHKVV"
     gene            20449..21435
                     /locus_tag="PK015_00930"
     CDS             20449..21435
                     /locus_tag="PK015_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOL33069.1"
                     /translation="MDIQELNFKVATSSALLDNLRTEIAKVIVGQDAIINRLLIGLLA
                     NGHILLEGVPGLAKTLIISTLAQAFDCVFKRIQFTPDLLPADITGTLIYNQKTGEFTT
                     KKGPIFANFILADEINRAPAKVQSALLEAMQERQVTIGDTTYPLPRPFFVMATQNPIE
                     QEGTYPLPEAQVDRFLMKLKIKYPTLEEEKVILERMVVERELRVNPVLKPSDLDYMRE
                     ILADLYMEERLKHYILNLVQATRYPQRYPRISSLTGLIQYGASPRATIFLARAAKANA
                     YLEHRAYVIPDDIKAIGKDVLRHRIILSYEAEAEQVSTEDIITRIFEEIEVP"
     gene            21448..22344
                     /locus_tag="PK015_00935"
     CDS             21448..22344
                     /locus_tag="PK015_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOL33070.1"
                     /translation="MSSPTPAEILARIGKLEIRTKNLVNEIFSGEYRSSFKGQGLEFA
                     EVREYQAGDNYRDIDWNVSARLGAPYVKKYTETRELNVFFIVDVSASMQFGTVQPKKQ
                     ERVAEIIALLSFSALSNNDKVGLIMFSDQLEKYLPARKGSNKALEILRDVLYLEPEHK
                     NTSLLEALTFANHILKKRSVVFILSDFMDSGYNKPLEILAQKHDVIAIQILDEAELKL
                     PDAGVLNLYDPETGDTLYCNTSATFIQKAYEARVSKQQEDLTKDLAKMKVDYIMLRSR
                     DSHIKALRTFFEQRRRRLREGR"
     gene            22341..23261
                     /locus_tag="PK015_00940"
     CDS             22341..23261
                     /locus_tag="PK015_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33071.1"
                     /translation="MKKILPLALILLFISYLSAVTIQQKLVNDDNLNVGDRFQLQIYA
                     DVPLKSVVVPDTLTSFKIIEVEKKKEPSGKTWLQLTIVPILTGSLSFPKLQVIPEISD
                     GQTYFTDAFRVIVIPVRAENDTTLVDIKPLAKYPLQLPIWVYYLLIALIIVGIIYLIL
                     NRRNRGGKDSTVKETIPSSTSLDPPWKIALKQLDELLSQNLIQKGEYIRHHYELSLIL
                     RQFLERKYRFPAVEMTTSEIQQVAERLYIASSQEVLNFLKYCDRVKFAKYIPSPEEIE
                     EYENWLRSWLQSFELVEAQQKLAHGGIEHA"
     gene            23254..24249
                     /locus_tag="PK015_00945"
     CDS             23254..24249
                     /locus_tag="PK015_00945"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOL33072.1"
                     /translation="MLSFARPWWLIALIIVPLFLWYQFKIQKPHKLRLPFTRLPLLKQ
                     IKGENKFWKYLFPALRSLIFVCLILAIAQPRWGYGTRDISQKGVDIVLAIDISGSMLA
                     MDFAPQNRLGAAVNVAKDFIKKRPNDRFGLVAFSEYALTQSPLTFDHEAILEQLDRLQ
                     VNKEASFTAIGVGLAKAVARLKDSTAKSKVIILITDGVSNTGEIDPLTAAKMAKAYGI
                     KVYPIGVGTNGLVDYPIEDPLLGTYYRKMQVELDMNTLNAVAEITGTGKAAQAGNKAQ
                     LQQIMDKIDKLEKTTYQIQIHYIWSEKFMPLLWIAFILLTIELLLHLIVMPILPE"
     gene            24253..25227
                     /locus_tag="PK015_00950"
     CDS             24253..25227
                     /locus_tag="PK015_00950"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOL33073.1"
                     /translation="MNLYYPGNIWLLLIIIPLIVSLFFWNRREKKRFSRFANPSFYPE
                     YLSNRSQFLHFFKYILIILALAFIIFALLRPQWDYEEREYSFNGLDIMVCLDVSKSMD
                     AQDITPSRLLRAKMQIDSLIDKLKGDRIGIIAFAGVPTLECPLTDDYSCVRLVLNSIN
                     TDNVVSYGTDIGAALALASRSFQSAGGSNILLLITDGEDLAGSAILQAKRLSSQGVKI
                     YVLGVGSEEGTFVRDEKTGQQAFTKLDTKTLQAIASIGKGKYFSVRPGQGELDPILQN
                     IYASESGRERSRNFSILKDQYAIPAIIAIIILLVESLLLPLSRKRENV"
     gene            25224..25850
                     /locus_tag="PK015_00955"
     CDS             25224..25850
                     /locus_tag="PK015_00955"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOL33074.1"
                     /translation="MKDKKLLFYKLILILVILILLFLIYTLLFNQKSLKHSRASELYK
                     KGQFEKAAKIWEKELDSKDKDYLPESSLGKALYQQGKYEESIKKLQDALKENDKDPGL
                     HYDLGNAYLRNNQIDNALQAYLNAMLLDPDDENAKANYELALKKKNAILLQAGIGENN
                     KDQNQQNLPDQSLREEMENRLNALDQKEARDRMAKNPPPYKGKGEKWW"
     gene            25844..27667
                     /locus_tag="PK015_00960"
     CDS             25844..27667
                     /locus_tag="PK015_00960"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HOL33075.1"
                     /translation="MVKKFACALLIVLSLMGILKAESVTVNAYLKEDVITLSDQLQLV
                     LEITSDKQVKVIAPPAPQIPGFSFRNVVGSTYLDYSLVNKNLFTKYIQTFTYIYIPQK
                     TGTFTIPSFKVKVEKKEYYTPPLTVQVKESSPPPQKQYNLSPFLNPFGDELFNPNLIE
                     GEVFLLCIPETDLAYVGEPVIISYYLYTEQAVEAFYAESEKDFEGYGKAVFLQPSSLK
                     YESSVYNSKSYKRALIKQDVIFPHKAGRLQMPILTGQAQFLGFLNKTISSKPCWINVK
                     PLPSGAPAGFTGAVGNFTVSQSVSSTKLNLGDALTFTINISGRGNFSQFTAAPFPNVD
                     KFQVSAPYIQDKLANVIEGTRFIHYTLLPRETGELTIPGYTFSWFDTTSGVYKTFQGA
                     PQEIKVHQANVLSYFSGLLEKDKPQALYPLLNLEYYPDFKNYSTEPWFWLILALCAIS
                     LIISSLVARENRLKELEPVAYAQKTAHRILNKYLKKATEAAEKLSLDFYPLAEIGLMD
                     YLARKYGVSKSLPTPELIDALRTKGMPLELLEQLEQFLLLCQKARFMPGGAEAEKLDE
                     ALHQLRSLVQALSRFKPEKESSKKNRVTLPQKDINSKNEEQ"
     gene            27667..28431
                     /locus_tag="PK015_00965"
     CDS             27667..28431
                     /locus_tag="PK015_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOL33076.1"
                     /translation="MKHWIFIFGLLILTFSLSAQVLNPDSLNAEQAFYQQTLQKLEAK
                     AAHGVKNADLFYDMGVCHYQLGNTGKAVLYFLRALNLNSAHKQAKQNLLYIHNLHPEL
                     SPEPNRPFLVQLFLNIYDFFSLNRLAITLLILAILLTLSLHWLMHTPSEKERGLPILA
                     VVITAILFIAFGSMLLVKNHRYRNDSRAVVIANVAPIYHTPESNNVLQTIPEGSVVVV
                     IKTQGKKTQIILPDGTQGWVDSNSIERVVPVSNTKE"
     gene            28435..29391
                     /locus_tag="PK015_00970"
     CDS             28435..29391
                     /locus_tag="PK015_00970"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HOL33077.1"
                     /translation="MQKPKLFITRYIPEPAIKRLEEVFEVEIFPENRAITHEELKEKV
                     KDIDALICLLTDNIDHEIIEAAPNLKCISVYAVGYNNIDVETATKHSIVICNTPGVLT
                     ETTADLAWALIMSCARRIVEADRFVREGKFQGWEPMLMLGNDIFGKTLGIIGMGRIGR
                     AVAHRALGFDMKVIYYDPQVDPESLPSDYVSVDLTTLCQNSDFISIHTPLTPETRHLI
                     GEKEFNLMKPTAILINTARGPIIDEQALISALKENKIAGAGLDVYENEPVIPEELIKL
                     SNVILLPHIGSASYETRTKMALLAAENAIAVIEGKNPPARVN"
     gene            29710..29973
                     /gene="purS"
                     /locus_tag="PK015_00975"
     CDS             29710..29973
                     /gene="purS"
                     /locus_tag="PK015_00975"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HOL33078.1"
                     /translation="MLVAKIFVCLKPNVLDPQGKAVCNSLQQLGYTNVIEARVSKYIE
                     LTFDSDNEESIWKETDKICQELLANPNTEHYWFTLENKEESQS"
     gene            29970..30662
                     /gene="purQ"
                     /locus_tag="PK015_00980"
     CDS             29970..30662
                     /gene="purQ"
                     /locus_tag="PK015_00980"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HOL33079.1"
                     /translation="MRVSVITFPGSNCDHDAQEAFAYYGHKSRLIWHKDRDLQNPDLV
                     VLPGGFSYGDYLRCGALARFSPIVEEVMAFAKKGGLILGICNGFQILTECGLLPGTLL
                     RNIGLDFICQHQYIRVEDNNTAFTHQIPEGTVMDIPIAHKEGNYFCSDEILKELQDNH
                     QIVFRYCDKQGNINAEYNPNGSRENIAGICNRTRNILGMMPHPERAATPYVTSNDGGK
                     IFAALEKYFNLN"
     gene            30610..31338
                     /locus_tag="PK015_00985"
     CDS             30610..31338
                     /locus_tag="PK015_00985"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOL33080.1"
                     /translation="MMEEKSLLHWKNTLISIDKLFFPPVCLACNQRIESVKEVLCSEC
                     KELLSPITINYCDKCGAPMIDYSCPYCSENDFVFDIARAAFIYQSPVRELIHNFKYNA
                     FLAPASFFSQAIMELPEADKFANNFDLITAVPLHRVRKRERGYNQSELIAKKLAIKWN
                     IPYAELVKRYLPTPSQTTLNAEERVNNLKNAFRLEKNADVKGKKIILVDDVFTTGTTV
                     NEVSRVLKEAGASKVAVLTAARAI"
     gene            31341..31754
                     /locus_tag="PK015_00990"
     CDS             31341..31754
                     /locus_tag="PK015_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33081.1"
                     /translation="MNKEYKEIKTTITEEEANEMIEKVAHFFVDRSLGSAGIMLFESL
                     HPLHGIASQALYFILPFAEMIFDSNQYQRFALMIQSDDYFKRLIKRIDELDEETNEER
                     RNKARLKRQRRKNQRKAFFKKIFNKTNKSTESTEV"
     gene            31754..33616
                     /locus_tag="PK015_00995"
     CDS             31754..33616
                     /locus_tag="PK015_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HOL33082.1"
                     /translation="MQYDEKRLTRIVATDCGSTTTKAILIEWIDGEYRQTVRGEAPTT
                     VEAPLNDVTKGVINATQELEELARLKYHNPNIKFMENGEFVIPRRGDVGVDAYVSTSS
                     AGGGLQMMVTGVVASMTGESAERAALGAGAIVMDLIASNDKRMNHEKIERIRELRPDM
                     ILMAGGEDGGTIKHVVEMAELVSAADPKPRLGSGFKLPVIYAGNKDARDYVRECLQDK
                     VDLIITDNIRPKLEIENLKPARDKIHELFLDHVMKQAPGYDKLMKWCQGPDHEPVPIM
                     PTPAAVGNIMQAIAREENIEVVGVDIGGATTDIFSVFTEEFIFNRTVSANLGMSYSIS
                     NVLASSGLENIMRWVPLDIDEGYLRNMIKNKMIRPTTIPYLLEELVLEQAIAIEALRL
                     AFEQHKEFASTLKGTQRQRDISEAFSQSTSGETLVNLMTLDLLVGSGGVLSHAPRRSQ
                     TMMLLINAFLPEGITRLAVDSIFMMPHLGVLSEISTKAATEVFHKDCMIYLGTCVAPV
                     GKGKVGKPALTAKLELPDGSTWEEQIPFGEVRLIPCGVGEVAKANLKTHGGLILDENK
                     NKELEVELKGGVVGIVLDTRGRQPFVLPTEKAERIATLKKWIKAFNAYPEQILK"
     gene            33627..34757
                     /locus_tag="PK015_01000"
     CDS             33627..34757
                     /locus_tag="PK015_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33083.1"
                     /translation="MAQAYSAGLMVTENIVLKKERVLPLKGQVLVKKGDFVQAETVVA
                     ETLLPGKVVPFNLANKLGVAPAQLPNFIKVKPGDKITTNTVLAETKGLFGLGIMKNEV
                     RSPINGEVESISTITGQILLREPKIPVQVKAFIDGMVTEIIPEEGVIIQNKSAYAQGI
                     FGVGGETVGELMVIAESPESEITPAQITDACKDKIIVTGQFVSYDVIDAAKKHGVKAI
                     ITGGIDDQDLKKLLGYDLGVAITGHENIGITIVCTEGFGKIAMAKKTFSLLKKFNGHK
                     TSIHGRTQIRAGVIRPEIIIPLQFSEEELVPQEPKPPVLEIGTIIRIIRQPNFGKIAK
                     VVALPEELTKVESETLVRVLEAEFEDGTRMIIPRANVEVIEA"
     gene            complement(35440..36390)
                     /locus_tag="PK015_01005"
     CDS             complement(35440..36390)
                     /locus_tag="PK015_01005"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HOL33084.1"
                     /translation="MVKQVIKIVMLSVILLSCLFIVSCDNTSGPRFKGEVFTVAGLLM
                     ADKPISLSYPIYITRSSSIEEFDPLELFVWDADVLIKDLDTGETFSLTPSLHEFKIKY
                     IDLAEHIIQPEHHYRLEVQIPGMDSLIWAETTVPQRVELKPDPYGINPPGTGYSFDPE
                     TTNIIPFNQIDTNYPLVVSTGNTLGIFNLWIETYCMEEFSTNLEYTNQLLQEMHPSAN
                     IEELYNQGSLGIRRLELANRFQSAPLEGLPGYYVVLKNYSTAFIFYGKYRVRVWVIDD
                     NYYRFHYMVDGYLHGGVHNALGYFGSASGGELYARIIKSN"
     gene            complement(36384..38726)
                     /locus_tag="PK015_01010"
     CDS             complement(36384..38726)
                     /locus_tag="PK015_01010"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOL33085.1"
                     /translation="MNKWEEMQKKILLMVLIIFLCGILHCATISGFVSRADSGEPIQY
                     VNVYIADTKIGCQTNKKGYYVLNLQQTGDFILTFSLVSYKKETVPITIKSLNDHLVIN
                     MQLVKSSVELSPVIVKGTKDESDGPIIQPSTLHQTRQDIQMVVAPMEADVFRAVLAVP
                     GVVPISDFFAGLYVRGGSPDQNLILLDNTEVYNPNHFGGIFSTFNTDAVESIELIKGG
                     YPAKYGGRMSSVLDVTERDGNRKFYEGVGRLSLISASATAEGPWKIDSQSGSFMASFR
                     RTYLEMLKMIISDLPDYYFYDAHIKINWDIDTNNKLSFSGYYGKDVLSYDITPIMDLD
                     WGNSILSAQWMHLFSPQLFSQFIISGSQYNNSIDEVSAEGMNIFQSKNYIRDLTVKVT
                     LNWKPSNHHDIETGIELKFNDTDLKMGTTYQIAQNALPQAAITSLTNSAFTQDTWVIN
                     ELWTLQPGLRVNWYKTLKEEPAPVPSASYFNLEPRISLKRNLDVGESIYLNFGVYHQY
                     LSLLGLYINSPYDVWLPIDGSVKPGVSHHYILGYNRALSRYFQWDAELYYKTYNHLKE
                     FNTNTFFNWNSETGTLSDAVNTGKGYSYGVELMLRNDWKGLKGYLGYSYSETKRKIDN
                     VNIDPYTGEPQYFYPRYDRTHTVSLVENYNISQNTGWQIGNADMIVGMNFSYNSGQPS
                     EIPERIFFDGENFQLMYSYQDRVRLPEYLRLDLSVHLQWNTFWGSIEPYLEIINVFNH
                     KNVSFRFYDVQMNEDGTLKLVTRDGTQFPFLPFIGVNVRW"
     gene            complement(38861..41671)
                     /locus_tag="PK015_01015"
     CDS             complement(38861..41671)
                     /locus_tag="PK015_01015"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOL33086.1"
                     /translation="MKKGWLIILALILVTAMFANGSNRYEIIAWQETFENGAEGWTHF
                     DASVPPNQWHIYNYGGTQGTVWWMGDPALAQGTNIGGYYNHQYLVLDTPARTISAANS
                     VLTFKMAVALEEVGGTGVYNGWDSFNVRISTNNGATWTVLTPTSPPYDFTSSYAFGFE
                     HNEGPNIPGWGGIHPAWTNVSFNLSSYVGQSVKIRFAFASDPAYCTTDDPTLFGVMVD
                     DISFGGYTNNGVDDGQMTWASLVAVGGDLWGLATDPTAPSPPHVMRCQNENGTYNPNM
                     MNYLVSPPIQLPNEGDIRVDFMIQGNFSDPDAFPNCDYWGWEISPNNGLTWYYMSNPY
                     GDPNLSNYVYSDAPPTWASMVASYAGVDGYISDYAGHTIRLRWLFKSDADNPIGTGIM
                     IDDVTIYNEVFIAPPENLEAVVSGRDVMLSWAAPGTGGGGGQEGWLHYDGENADAIGT
                     GSAADFAVAAKWDPIGEHSIYDYVGMNITKVRFFPYEANCTYTIRIWTGATGALVYEQ
                     AVPTPTINAWNEVILTTPFTIPAQTVIMVGYRCNTQTGYPAGCDAGPQVEGYGNMMYW
                     QNSWTTLTNLNSSLTYNWNIRIYVANAKGEEYELSYAPLKQEVQPVSNAQFKAKGIKA
                     KRNVDGYRIYRDNVQIDQVGADVLTYTDSNVPGGIHSYYVTSMYGTNESGASNVVTVF
                     VMPESMVELSHDDGTADQGFNVGANKQMAVKFSFNRSLTVKYAKIYIQNVGNAGLIVR
                     VFDNDGPDGMPGSQLAQYSYPVASLVQGWNYIELPPDIDVQDGQFYIGILDVANGSAI
                     GLDTSTSGNSFKKMGTTTNWEPITEGELMIRAIVWYDTPNDDNVIPVYTLSATNYPNP
                     FNPETTISFSVPTSGYTTLKIYNLKGEVVRTLVNDVREAGKYNVVWNGTDDRGRTVSS
                     GIYLYRLENNGKKLTGKMLLAK"
     gene            42031..44466
                     /locus_tag="PK015_01020"
     CDS             42031..44466
                     /locus_tag="PK015_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOL33087.1"
                     /translation="MFLTDHQLKSEILRCEYCAEKPCKTACPCDCSPADFMMAAAEGK
                     PSDYKRAAGIILSSNPLGGICGKVCPDDFCVQACSHYKFDFPIRIQEVQATIINKAHL
                     LGQLPEFQQVPKNGKKIAIVGGGPSGLGAAVTLTQLGYEVDIFDAEPLGGQIRLIPPE
                     RLDTEVINNDLNYLNRAFNFTHIQEKVDNVQELFSKGYNAVLLACGLNEPIKLNIPGS
                     ETEVLGLELLRNPEAYELKGKRIALVGGAIAADAALISLRKGANYAEMITLEAYNEMP
                     ITEDEKQKLIEAGVAFSPRTRITAIINENGKTVGIKTKRVTLPSGLTFHPSNIVDEEG
                     STEMYRPFDMVVIAIGNRCSLNEQGTHIYQCGDMVNGPSTVVEAVASGKNIALKIDAD
                     LRNQDYTEPEKQTKSIISIPHWKQYPVSLETDFFGRKIKSPFLLSAAPPTDGYEQMKA
                     AYLAGWQGGIMKTAFEKQYIHIPGEYMWVFNGKTFGNCDNVSEHPLDRVCEEIQRLIK
                     EFPDRLTMASTGGPVTGNDESDKAGWQSNTLKLENAGAMGIEYSLSCPQGGDGTHGDI
                     VAQNAELTAKIIDWVMEISNPEIPKLFKLTSAVTAIVPIIKAVKSVFNKYPEKKAGIT
                     LANTFPAMGFREKEAALWDEGVIIGLSGKGILPITFLSLARACNLGVIVSGNGGAMSY
                     LDAANFLALGTSTVQFCSIVEKYGYGIIDELHSGLSYLLEAKKLKSVQELIGIAQPNP
                     ITDFLQLPPKSKSSTLNQNLCLQCGNCTRCPYQAITLNEDKYPEIDKDKCIGCSLCTK
                     LCFTGALYMSKDS"
     gene            complement(44515..45153)
                     /locus_tag="PK015_01025"
     CDS             complement(44515..45153)
                     /locus_tag="PK015_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HOL33088.1"
                     /translation="MQYSQIVLDHFMHPHNVGKLENPDAEATEGSPACGDQVTMFLKV
                     NPETKVIEDIKFLSYGCASNIATASIITDLAKGKTLDEAKKITWQQAMEELGGLPPVK
                     VHCAVLAVDTLQSAISNYEIAHGLKEVPNFGKDTIIEELRKIIYPKVGEDIVTLKMVK
                     YVGFNDGEVIIDLNLMNFDQWRDNIAEEIREHLKKYAEIKNIQINFPDSQAT"
     gene            complement(45154..46347)
                     /locus_tag="PK015_01030"
     CDS             complement(45154..46347)
                     /locus_tag="PK015_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOL33089.1"
                     /translation="MNNHRIYMDNCVTTPLAPEVLEAMKPYFLEKFWFPGTFISTGEE
                     TNAALAEFKEILAKALHSLPEEIHFTSGGTLANNIALKGLMGANANKGRHIIVSLVDY
                     PDLLTNAAYFEKQGFEVSYLEPDSEGWVSADKLKEAIRPDTILFMSTIVNHVVGTIQP
                     MEDYKKVIEAADHKIFWHADAGQAFGKMHLDVQDLGVDTLSVSAHKIHGPQGIGALFV
                     KKGTKLSQLIHGVTRVDQLQTGGLSIALIAGFAKAVEMTFNDLDANISYLRELSDYLL
                     GKLESTIPDIELNGPRGDKRVCHNINVSISYIEGESITMMLDLNGITVATGSACASQG
                     LKPNYVLMAMGKNHVQSHGSMKFTLSRYNTKEEIDYTVEKLAEITATLRERSPLYQAI
                     HNKER"
     gene            complement(46347..46757)
                     /locus_tag="PK015_01035"
     CDS             complement(46347..46757)
                     /locus_tag="PK015_01035"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOL33090.1"
                     /translation="MPVSTKTEYALRALVEIAEHPEGTISAQNICNQQQLPKKYIEHL
                     LSSLKNAGLIISSAGLKGGYVLAQSPDEITLYDVMKAVDDHRLELDCNMGRKFCQGED
                     CPLRPIFIEIGEKQRELFKSYSLALIAEKMREED"
     gene            46893..47669
                     /locus_tag="PK015_01040"
     CDS             46893..47669
                     /locus_tag="PK015_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOL33091.1"
                     /translation="MIEIEKLCCAYEDELVLKDVDLILPENEFTVLAGPNGAGKSTLI
                     YALLGFLKPRKGKVRIFGRPLYSYKRIELARLIAFVPQESIFQFDYPVRDIVLMGRYP
                     YLGLTQNWMAEDYEAVDKVLQQLSLQDLAHRFYSQLSGGEKQRVLIARALAQDTHYIF
                     LDETLSQLDINHQIDIMVLLRKILAENKKGILLISHNLNLSANFADRMVFLSRGTILG
                     SGTPEDMMKPELLKELFGIELLTMLNPLSGKPNILYPGMK"
     gene            47666..49816
                     /locus_tag="PK015_01045"
     CDS             47666..49816
                     /locus_tag="PK015_01045"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOL33092.1"
                     /translation="MKSWIVTLCLAIITVALTAESYLIQAVVVDPKEHPVSEVVISDG
                     SKTVFSNEKGYFLINTSADTLTFHRLGFQEKKLSVKNIGKKVILTPEPVVLPRITVSD
                     VAWNIVSPPPDRISLPIDPDRHYYSAGEVLTTNPAIHSNDVPLPGESQNISILGNLSR
                     HSLIILDGVPLNPDGSSFDLSLLDPNNIESVDIIKNNVSVYGGSSAIGGIVMISTKKG
                     LQQTGKQFSISTELGSFGYAQNSLSFTFNQPSTIFRINLSNLSTDNDFRYKVPEWWSP
                     DSTAIRSNNAKHQNSLAASYTHLNKKSHFSFQTEYLSFMRQLPGTVNFTEIYKNASLS
                     GWANRNRFNLNAPLFSGELGTLIWLNLDQTTYDNTKAPLPVYLSHYKQSLLNAGIHGS
                     YGQNSSLTPELSLNTSLSAEAGYNSYQNNNLLNSANNIDFSSRFANSILKTELKMDKG
                     EFIWSNSGAIRYDLTEQENEFTWRLESSLQSLGYIETTIGGTLGTSFALPSPYDLYWK
                     GDSQALGNPDLKSEHSQGWQLWMSNQWNKLYLKSAFHKNDIENLIQWRQIQMWGNVWK
                     PLNIGKARIKNVEIEAGWQPWEWLNLSTSALFTNAKDISEHNEGAAPYLVYIPGRNYS
                     LKMELNWKKLKLWSDYHYSGKQYTTPDNLTEPFPGYSLLDLGLNYSMFIQGWNVSPFF
                     TIRNALNKQYEIYAYVPQPGIAFYGGVSLQVSNP"
     gene            complement(49849..50742)
                     /locus_tag="PK015_01050"
     CDS             complement(49849..50742)
                     /locus_tag="PK015_01050"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HOL33093.1"
                     /translation="MLKIQEINPEIVKLHILGFSGTEELPIYGLEIGYSSAGKKVLMI
                     GQHHSDEVLGVEIVVSWAEKLAKEAESDKKIKALLHQYKFWIVPTINPEGYRVFTEGL
                     YRYKRKNNRDTNQNNKLELQWDGVDLNRNYPVFWDEDSTLPPTHRYYKGDTPASEPEV
                     QAIISLAQKQHFDFAIFYHSSSTGAYSEKIFLPYLDEENENQKRIYEETLEFAQKLAE
                     NLKKDYLPGKYEVSMATSSRVGNARNYFFHIHNTRAILIEVGGIDKKGKSIIYPSARM
                     KDKIVKKHIAALEKVFYESLE"
     gene            51490..52848
                     /locus_tag="PK015_01055"
     CDS             51490..52848
                     /locus_tag="PK015_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOL33094.1"
                     /translation="MKGVRQIAVDQLVNYLATDPIQKMSKIMSLAKKLDSKHQYEYYI
                     DGIYNALMDENSNWHKFTENLFKEVNTKSIRKLAECFMVNSTLVGGPRRDKIKAKYNC
                     NVPWALLIDPTSACNLSCTGCWAAEYGKKYNLSYDILDSVVKQGKKLGIFWYLFSGGE
                     PLIRKDDIIKLCAKHQDCFFFAFTNGTLVDEKLCEDMRKVGNFVLAFSIEGDEEATDM
                     RRGKGCYQKVISAMELMKKHHLLFGYSTCYHHYNTESVASDEFIDDMIARGCRFAWNF
                     TYIPIGKDAKLDFIATPEQRAYMYKRIQEIRATKPIFALDFWNDGEAAHGCIAGGRSY
                     LHINANGDVEPCAFIHYANVNIKNATLLEALQSPLFMEYKKHQPFSENMLRPCPLLDN
                     PEILMEMVHNSNAYSTQLEGPETVDELCAKTIAAAEKWAPVADKLWEERQKELASRNP
                     SP"
     gene            complement(52919..55087)
                     /locus_tag="PK015_01060"
     CDS             complement(52919..55087)
                     /locus_tag="PK015_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOL33095.1"
                     /translation="MKGGGDNGIGRPVIEQDEIKNRIFDRRLYGRLLHYLKPYIAGVI
                     GSFLIVMVVSLAELVQPLIQRRAIDDYIVSDKNIAVFQDETTANSFLNKYSYLNLNRL
                     TYEGRYFVILNSADLNKINQQDILEFKNKGIIGEEKVFLIVDKPENIQILNKYLPEDQ
                     NPKGGKEGFKGWFRVGDGQIAISREQLNKVPKSERFQLRNEAANKLIWLALAFLIISI
                     IRFIAGYFQVIITTIFSQKAMNDLRHDAFAHLQKMPVKFFDVNPVGRLVTRVTNDIRA
                     IDEMLSSGVITIIQDIIMIIAIVVLMLLLNWKLALVSFAILPGVVWVIVIFRKRTRPV
                     YREVRKHLAAVNATLNEHISGQKIIKLFNQYKRKREEFARINQEYYNASMKQMKLFAF
                     FRPIIHVSSQIAVALLIWYGGGQILRNAITIGLLMAFTQYISKLFQPIDDFSEKFNVL
                     QGALAGAERIFDLMELDPEDYREHLKTGIRLQGAIEFKNVWLAYNPDEWVLKDVSFKI
                     EPGEKIALVGHTGSGKTSIVNLILGMYPYQKGNILLDGKPLESYAVEDIRKNVGIVQQ
                     DVFIFSGNIRDNIALNNKQLSKEQIIQVAKYVNADKFISKLPLGYDEPVMERGATLST
                     GQRQLIAFARVLAYDPAIFILDEATSNIDTETELLIQDALQKIMHNRTSIVIAHRLST
                     IQNSDRIIVLHKGVIVEEGSHFELLEKKGLYYDLYRLQYT"
     gene            complement(55084..56835)
                     /locus_tag="PK015_01065"
     CDS             complement(55084..56835)
                     /locus_tag="PK015_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOL33096.1"
                     /translation="MRTFDAVIPYLKKSYGRIAGGLVMLILVDIVQLITPWVMQQAID
                     RIQERTIDSRGLLLLALVIIGLAIAVMVLRYFWRVLIIGNSFMIEKNLRQDFYDHLMQ
                     LSQNFFNKRKVGDLMAYATNDLNSVRMMFGMGLIAAMDIVLMTIASFSFMGSIDLRLT
                     ALAVIPMPILTITISIFGKKMHKSFYRVQTSFATLTGTIQESISGIRIVKAFVQEKSE
                     LEKVDKVSWDFVKENINMAKIVGFFHPFQGFIISLSMIITLYFGGRATIRGDITIGQF
                     IAFFQYLGMLVWPMVAIGWIVDMYQRGTASLKRLNEIFETESEIDDKEADFSIKALQG
                     NIEIRDLTFRYAKNLPLIFDHISTSVEQGKTLAVVGPTGCGKTTLIELIVRIYEPPRG
                     SILIDGHLVHRIPLQVLRRDIVLVPQDIFLFSDTIANNIRLGNPDAPIEAVYEAAKKA
                     EIYDEIMEFEDKFETVIGERGVTLSGGQKQRVAIARALLTNPEVLILDDALSAVDTKT
                     ERYILENLVEDRKGKTNIIIAHRISSIQHADNIIVLSDAQIIEHGTHQELIAKGGLYS
                     DLYEKQRIRARLEGEEL"
     gene            complement(57137..62590)
                     /locus_tag="PK015_01070"
     CDS             complement(57137..62590)
                     /locus_tag="PK015_01070"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOL33097.1"
                     /translation="MIKKVFLIIMLGLSLSAILALNDNVISQTNKAFQITTKAIDHMD
                     IQFNLPSYEIIEEEAGGNVYQRILIPEAGVTMDSGLPELPTINIMLAIPRQGKVQIET
                     LNTQTQVLPQFLPYPVQQGQELESPKSFVIDSAYYENGASYPANLIQVSDPMILRDFR
                     IIGIQVNPFSYNPQSHTLTINQSISFRVNYLNEPGINELEGELQSLSPAFANIYESII
                     FNFDDYRDLIDTHIPPRYLIIYGYNSDTNYISSINSFALWKRQKGADVSLASTASTEA
                     GSSTTSIKSYIQNAYNNPATRPDYVILIGDTGGSYTIPCFTYSNGATDYPYTHLAGND
                     LLGDVYIGRISAENLSQLQTIFAKIYLYERDINLNNAQWLNRMLLVGDYSPSGVSVVY
                     TSKYIKECALNINPDYTFTELYGDDPSPSLANSAINTGIGFHSYRGYINMSGWSPSDS
                     LNNGYKLPHAIIITCNTGNFSSTATTESYIRLGTSAQPKGAVTAMGMATSSTHTTFNN
                     ALHGGVWGGILQYNMRTPGEAMLNGKLFINQIFGVSSPSNANNFAHWLNLMGDPTMEI
                     FCGIPNHFNINSITTIPLGLSLMDVFVTDGTGEPVKDACVTLTQNNVIISRGYTDEDG
                     NIILILPSNMTVNPCTLTVSKHEFKPLQQSIAVDNSGTLVPGAIIVDDDNIDFSQGNG
                     DGLVDGGETIEFLFGLRNTSTSSISGISGYLTCNSPYVTITDSLLTFGEITPSSLGFN
                     NNPVVIHIAYNTPNDMMLRFHLILTDSNNISYNVSEFVKVNNAEMIFNSYQVIDGGNG
                     VLDPSENAGFTVTVTNQTTIPVTDIYGRLYTLNDLINITDHTAFYGDLFPNIEVTPTT
                     DIFQLYGRPLLLPGMIIPMRLKLYNSFGFEQWLDFSLTIGEVTVNAPLGPDGYGYVIY
                     DDQDTGYEECPVFNWIGIAPAEGGLGTALAISDSYTSSNEGDQVGSDALEVVDLPFPF
                     QFYGRIYTQITVCSNGFIAMGVTENAEFRNYRLPGPMGPSPMIAPFWDDLATHSGGGI
                     YTWYDRNNHCFVIEWYNLKNGSNGSSLETFQVILYDQSMYPTSLGDGPIKFQYQTFNN
                     VDMSTGNRHGCYATIGIEDHSGTVGLEYTFNNVYPTAASPLGNQRALYITNVPLYYYD
                     PHIILCETYIDDSNGNGVCEPGETINLGIKLQNIGNQTAENVTATLITNNPYVNIITA
                     TGTYYPLTTDSYGVNQQPFVFTIAPECPNGTVINFRLQINSGDFSWQRNFSIRVEASV
                     MMFVSYLINDADANFNGIIDPLETVKLIVNVHNNSDVQAREVMATLSTSSSDVMIVNP
                     IISLPMIEPNTIMQFVFEVQFTGVSSLSQYISMQFNLSISNGLPISNNLLIPYNIANV
                     FNDFESNDGNFISETGWEWGIPAQVGPYSGVKVWATNLSGNYPNLIDYVLITPEYTLE
                     SSCILTFHHIYGMENYYDGGNVCISVDNGNNWTVITPLGGYPHNSIVSLNSQPGFTGS
                     INSWQTVQFNLSQYAGQTVRFRFRMTSDSATTGIGWFIDNFELSGVNQKTGYIYGLVT
                     PTSTTPASKALVMANNHFATHPDDNYSYRLYLPFGTYNVTATLLYHQSSSAFNIQITP
                     ESPLRQTDFTLIDLPKPVNSDFVVNNETGELLISWEPPYDPVLPIGGYKVYKKFNTGP
                     FELVQTTNITSYTDYLSLNGLYKYYIRALYYNMEGCPSDTIEFIFPYQNVNEPSTPGL
                     ITRLKTNYPNPFNPTTTISFDLAEAGKAKLSIYNIKGQLVKVLVEDNFTPGSYNVVWN
                     GKDSRNQNVSSGVYFYRLETKNKVLTQRMMLLK"
     gene            complement(62652..64073)
                     /gene="glnA"
                     /locus_tag="PK015_01075"
     CDS             complement(62652..64073)
                     /gene="glnA"
                     /locus_tag="PK015_01075"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HOL33098.1"
                     /translation="MTKEKIKDFIREKEIQAVDLKYCGMDGKWYHITIPARGLKEVLD
                     SGVPFDGSSIPGMKGVESGDMILMPDLETAQIDPFYDNYTMRLICSICDAETRQGVKK
                     DSRSVALRAQEYLLSTGIADKSTWIPELEFYLFDSVKYQSSEYNCGFLVESSEHKEAL
                     PEDNEDNDGLAQQDHKGYHMDTPFDQFYEIRQEMVDRMENYGIKVRYHHHEVGLASQQ
                     EIETELLEFPKICDDVMVMKDIIRRTALSYGLTATFMPKPIYNQAGSGMHFHIMLHKD
                     GTNIFYQKGGYADLSDLAIWFIGGILYHGRSLVAFTNPSTNSYKRLLPGFEAPVKLFY
                     GLANRSAAIRIPRYANSPETKRFEFRTGDATCNPYLAMSAMLMAGLDGIKNQIDPAKY
                     NLGPYDDNVFEWSEEKKSKLLSIPANLKEAMQSLKEDYHYLLEGDVFNEELIESYIKL
                     KLKENDEVNNRIHPYEYILYYNL"
     gene            complement(64088..64732)
                     /locus_tag="PK015_01080"
     CDS             complement(64088..64732)
                     /locus_tag="PK015_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOL33099.1"
                     /translation="MSFIKNTLTLIIILIIFYSIILHEISHALVSYWQGDDTAKMMGR
                     LSLNPFKHIDIFGTIILPLLTYFTMGIILGYAKPVPLNPYNYKNIKVGTGLSALAGPV
                     ANILIAIVFALIFHLTQGQQIIPYICQMVVFFNLLLAFFNLIPIPPLDGSKVLGMLLP
                     DAAFYKFMAQEHIGMITIIFILLVSGLLGFNIIGRLIIPPINFTMQLLGVGGIF"
     gene            complement(64750..65952)
                     /gene="tyrS"
                     /locus_tag="PK015_01085"
     CDS             complement(64750..65952)
                     /gene="tyrS"
                     /locus_tag="PK015_01085"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOL33100.1"
                     /translation="MNLEKELKIIRKAVDEIIPEEDLVRKLIRSKETGKPLRIKFGID
                     PTGTDVHLGHLVPIRKMRDFQDLGHIGVIIIGDFTAQIGDPTGRDETRPPLSHQQVLA
                     NAERFMEQLYTVLKQEQTEVRWQSEWFGKMTVEDVIKLMGNFTLAQFMAHDTFRTRYE
                     QGLPLGLHELMYPLLQAYDSVAVQSDVELGATEQKFNILCGRDMQRFLGQEEQVAILT
                     PLLIGLDGVNKMGKSLNNYIAVFDPPEEKFGKIMSIPDNLILNYYKYAAWADEEELEK
                     VQKELESGTNPMILKKQLAHRIVAFYHGNKAADEAQRAFEKVFSQRELPDEMPEYKVN
                     NSVMRIAKLLTESGLCESGSEAKRLIQAGGVSIDGEKITSTDAEIEIKEGMVLRVGKR
                     KFLRLRKQ"
     gene            complement(66048..66491)
                     /locus_tag="PK015_01090"
     CDS             complement(66048..66491)
                     /locus_tag="PK015_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33101.1"
                     /translation="MKANIKRGKTPPVIDSEKYIQYYHPNLHCTIIRRKPQMPHQPQN
                     DLYKQISLNLKALNPSPAYKSDFQIYTTLLRQKDENLRIPSWYPLFVKLMWNMQAKYP
                     EQVDLKTITKEEIISKNLPCRSVKAAIEDGLLTPIEGWEFLDHTI"
     assembly_gap    66820..66919
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(67489..67893)
                     /gene="mce"
                     /locus_tag="PK015_01095"
     CDS             complement(67489..67893)
                     /gene="mce"
                     /locus_tag="PK015_01095"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HOL33102.1"
                     /translation="MIKHISHIGIAVKNLDEAIAIYEKLGLQLEGIEEVPSQMVKVAF
                     FPCGETRLELLMPTSPESPIAKFIENRGEGIQHIALAVDNVTEELNKAHENGFRLIDT
                     QPRPGAHNSEIAFLHPKSTLGVLIELCQEKED"
     gene            complement(67902..68447)
                     /locus_tag="PK015_01100"
     CDS             complement(67902..68447)
                     /locus_tag="PK015_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOL33103.1"
                     /translation="MKYPYEIRLSGSGGQGLILAGIILARAAVLENHHVTQTQSYGPE
                     SRGGYSYADVIISNEEIYFPEAVSFDCLLALNQESCDKYLFDLRENGILIVDTTFVKN
                     IALVVENTYEVPFTEIAIDKLGSPIPTNIIALSFLVKVTDIVKIAFLQKAIEESVKPA
                     FIDLNLRAMKLGIDLAKNYEK"
     gene            complement(68444..69286)
                     /locus_tag="PK015_01105"
     CDS             complement(68444..69286)
                     /locus_tag="PK015_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOL33104.1"
                     /translation="MPAIPQKIIHEYLRHDKKFPHVWCAGCSNGIVLGSIIRAIAGMN
                     LDRNDIVMVSGIGCSSRMPVYVDFNTLHTLHGRAIAFATGVKLHKPHLKVIVVTGDGD
                     CAAIGGNHLIHAARRNIDLTVIMLNNNIYGMTGGQVSPTTPHEAYATTAPYGNIEPDF
                     DVCKLAMGAGATYVARTTAFHTQETQNMVKEAIEHPGFALIEVICACPVIYGRLNKKG
                     GAPEMMKEMRDNSIPLAAVDKLPPEKVQGKIIRGVLHKDIRPEYCAQYAELMKKAQAQ
                     GGSK"
     gene            complement(69288..70520)
                     /locus_tag="PK015_01110"
     CDS             complement(69288..70520)
                     /locus_tag="PK015_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOL33105.1"
                     /translation="MSNSETINPNTTMNNENKTSSTTKVEELQNLKGRKTVLMQGNEA
                     VAYGALDAGCNFFAGYPITPSTEIAEILAAELPKRGGVFIQMEDELGSICAVIGASLA
                     GAKALTATSGPGFSLMQEGIGFARSAETPCVVVDVQRVGPSTGMPTSCAQGDIQQSRW
                     GTHGDSPAIVLYPDSVKESYELTIRAINLSEKYRTCVILLLDEVLGHMREAVRLPDLS
                     NVRVISRVKPTVPPHWYKHYEDNEKYPIPLASFGEGYRFHVTGLTHDSLGFPTNKPNE
                     AAEMMARLKKKITYNIRDLVQIESFQMDDAKIAVFAAGITARSAKAAVVMARKEGIPA
                     GLLRPLTIWPFPDDAVRKMLRNVDIVIVPELNQGQLIREIERLVKDRSDSKLIKIQRV
                     DSKLIPPQDILQKIKEVI"
     gene            complement(70530..70829)
                     /locus_tag="PK015_01115"
     CDS             complement(70530..70829)
                     /locus_tag="PK015_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOL33106.1"
                     /translation="MPKKVEKDRIGMIIREEDAPEKMEVELPEIKESGDKSSPVIIYY
                     NWCKKCGICVAFCPTGCLGRKADGSPYVLAPEKCTHCETCDLLCPDFAITGAKEH"
     gene            complement(70872..72092)
                     /locus_tag="PK015_01120"
     CDS             complement(70872..72092)
                     /locus_tag="PK015_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOL33107.1"
                     /translation="MINDLQDILSVNIRGMKRSAIRELLKYLNQPGLISFSGGFPAPQ
                     SFPVEELKGIIAEVMENEPYYALQYGTTEGDNLLRKQLADRYKKFGIDVNMDNIIITT
                     ASQQALDLIAKSFIDRGDTVIVGLPSYLGALSAFNSYGADMIGIPMDDEGEIPEVMED
                     EIKKAIARGKKPKFIYVIPDFQNPAGMTMSEKRRKEIIDIAHRYDILIVEDSPYRELR
                     YEGENQRTLYELDGTQQVVLLGTFSKIFCPGFRIGWVMGHPDVLDKVVIGKQATDLCT
                     PPFTQRIAARYMERGYLDPKIAEICNMYKSKQKVMLESLEEFMPEEFTWTKPQGGLFL
                     MATGPEWIDTDALLLDCIKEQNVAYVAGTSFFCNGKGQNTMRLNFSYESEENNREGCR
                     RLGTFFKKVIAEKS"
     gene            complement(72089..72400)
                     /locus_tag="PK015_01125"
     CDS             complement(72089..72400)
                     /locus_tag="PK015_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33108.1"
                     /translation="MNIHFRDVQTGSVEARAVLEIADGVFLNEITILNIEGEIVVEFP
                     KKSFVGKNHRMYYIDIITFEDNDKRLVWELEIKNAYKEWRKKNKKVLVYKQNKIDGGS
                     K"
     gene            complement(72487..73467)
                     /locus_tag="PK015_01130"
     CDS             complement(72487..73467)
                     /locus_tag="PK015_01130"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HOL33109.1"
                     /translation="MLFINSPSHYAPFNIACEEYILNNFPEDVFLLYRNDASIIIGRH
                     QNTLAEINLDYVKEHNIPVVRRLTGGGTVFHDLGNLNYCFLMNRTGDEDRGFAKYTAP
                     ILAALKDLGIEAKLEGRNDLTIKGMKFSGNARAVYPNKIHQHGTILFSSKITDLSAAL
                     KANPLKFKDKAVHSVAARVTNISEHLTKPISLEEFIQLIQKEVLKLYPDAREYHLTED
                     DKKGINTLMKSKYESWEWNFGHSPAYNHKQSIRCSAGTIEFHIDVHKGKITGLRIYGD
                     FFGERDPSELESALIGLPHRTEELRNALESLPLNEFFGEVTVEDILAGLI"
     gene            complement(73709..74101)
                     /locus_tag="PK015_01135"
     CDS             complement(73709..74101)
                     /locus_tag="PK015_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33110.1"
                     /translation="MKRFLLIMLSFSLCWGLNAIVTEVGSFRGFFYGSEPACEYDNWT
                     SHIAEGILTNTNVYAPWEVQNNNFGNYIQPTDTMLNQWEEVVVDFLNLNLDSAQAKIN
                     QYGFPYEIVQFQDLDSGRNLFFRETLNI"
     gene            74292..75434
                     /locus_tag="PK015_01140"
     CDS             74292..75434
                     /locus_tag="PK015_01140"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOL33111.1"
                     /translation="MESFSFYNPTRVLFGKGQIGKIGKELKKADIKSCLMIAGGGSIR
                     KNGVYEQVCESLKENKIEWIESWGVQPNPTVEKVREMITLAKESSVDAILAVGGGSVI
                     DSGKATAAGIFLNDIWNAYVDKEKIIRALPIFTILTISGTGSEMNGNSVLTLSSEKKK
                     WSISSKLLYPKVSIIDPSIQATLSFRQTVNGAIDAISHILEYIFMNETAIVTRNIDAG
                     LIKTIVKMTDRLQQVANDYTARANLAWSATLALNGISGVGLQGGDWACHSIEHALSAI
                     DPKIAHGEGLGVIFPAWIEYVSEREPNRFSWWVKEVCEEDSVARALRKCRETLKRWGS
                     ATNLRELGIRESQLPEIRDLLLTYGPIGEIVRFRAEDIEALLMLAY"
     gene            75512..76450
                     /gene="lepB"
                     /locus_tag="PK015_01145"
     CDS             75512..76450
                     /gene="lepB"
                     /locus_tag="PK015_01145"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOL33112.1"
                     /translation="MKEQNRVKSKLKKQPYKEPTYTITPIPPGKKFHCRKPWLQDWIE
                     AILFAFVVAMIIRNYTFQNFMIPSSSMEKTLLTGDYIVANKIKYYIEDPKREDIVTFR
                     YPKIEEGTPEHPDYKDNFIKVFPPIYINKKAAFSKHPMTFMHIAYYARKNIVKRVIGM
                     PGDIIEIKNKKVYINGELFERGYECYGIAYPPPPQPPFQIIEQTFAEDEEDLYISNSD
                     WYKPYIVPPDSTRGISARKVFNRDWFGPIKVPEGHYFVMGDNRDVSEDSRYWGFLKRS
                     DITGSPWLIFFSRGIEYNKLYDTPHIRWNRIFRLAR"
     gene            76420..77562
                     /gene="hemW"
                     /locus_tag="PK015_01150"
     CDS             76420..77562
                     /gene="hemW"
                     /locus_tag="PK015_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOL33113.1"
                     /translation="MEQDISSRQIAVYLHIPFCLTHCGYCTFFSVPYSKSSLELYLQY
                     LHKEIDLFFQYHPDLAYAKSIYFGGGTPSLLRAEDIAELCARFNFAEEAEITLEINPL
                     QITKNYLSALKNTPVNRLSIGIQSMDNEELNLLDRKHKAEQIKDKIELCRQFGFNNIS
                     LDLIYGLPGSSLNKLKDNIIQFLYLKPEHISCYLLTLDSDTPLAAKIKKGKVPRLPED
                     SLLAEEYELICSELDRAGYEQYEISNFCLPGKQSKHNLCYWKSEPYLGLGASAAGFLP
                     PYRYTNPSNLYDYYDHLDKGLIMPEAESCSSKQMIADFIMMGLRLTEGIDLNELKNHF
                     NYDLEEDKQSQLEKLYSLGMIEKNGSYLSLSTKALFVSNTIIGELI"
     gene            77559..78290
                     /locus_tag="PK015_01155"
     CDS             77559..78290
                     /locus_tag="PK015_01155"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HOL33114.1"
                     /translation="MTLRELRELLARAIKDTEFENHTYFAGGCVRDFLLNPDAAIVEA
                     DIAVELPEGGIKLAYFFQEKFNTPAPVIHKSFGTAKIIIEGIKIETVMTRSEKYPPGR
                     RHPYVQFADLYTDCLRRDFTINALYQEIMTGKVLDPCQKGFADLQARVIRCVQDPFHS
                     FVEDPLRILRALRFAVTLDFEITAETLSAIKTNAHLVSTLSANRCADEYSRIKQLPAE
                     KIAQWKKLLEETGVLNYLTAKIPIR"
BASE COUNT        24972 a        14944 c        14031 g        24448 t
ORIGIN      
        1 cctccatcat tagtgagagc aactaaacca tcgtctccaa ccaaaatgcc ttgcatctcg
       61 ttaatgaaat ctatatccca tacatcttta taattattag ccgatgcatt ataagctggg
      121 gtttccacaa atcgccaggt taaaccatta tcagttgact ttgctactgc acctacataa
      181 cctcccgccc aataagtatt accaattttt tccagacaat aaaaatttgt cggaatagtt
      241 gtctgtctga ttaagtccca agcaaaagcc attgagcata ataagacaat agtcattaca
      301 agtattaact tttttatttt ccctcctata ttggaataat taaaaatatg catattttga
      361 agtcatttaa tatttttcat atctaatatc tttatgaaag cattattcgt gcctaatgct
      421 gaaatttttt ctttcttttt cttatcttcc ctccattaat ttaagcatcc taaatcagca
      481 tataagagat acataattaa ttagaaaact cttataagaa gtaattaact ttcaagagct
      541 tcaaaaactt ccttttggat tgacaaaaaa caagtagcaa agaactttga ttaaatataa
      601 aagggagaat taatatggaa aatcggacat gggaatactt gagtgtattc attatgtcct
      661 tcttatttgt ttatggctta acgcctttta taattaaatt ggcacgagct ttaaactttg
      721 aagataaacc tgcagcaaga aaaatacacc agcagaagac acctcttttg ggtgggttaa
      781 gtgtgtttat tggtttttct ttattgtgta tatatgatgt ggcaatatca cctaatcgtt
      841 attttgatct ccctatgatt ggctatctac ttggaggatt acttattact gttttaggat
      901 taattgatga taaatttgga atgcatccag cgattaaact tctgggacag ataacagtca
      961 gtctgatatt tatattaagt agtttcagga tagctgaatt gaatcatatg ttcgggtcca
     1021 tctatatttc cttgccttta atggttctct ggatggtagg actgatgaat gcaatgaatt
     1081 ttttagataa tatggatggg attttaagtg gaatggcagg aatattaggc ttgggttact
     1141 ttgctttcag tttagccaac ataactggtt caaacagttc agaaatggca ttcattggat
     1201 tgatatccct tagttttgct ggtgcgacct taggtttctt accttataat ttcaatcctg
     1261 ccaagatttt tttaggtgat gctggttcta tgttcatagg atattttctc tcttctatgg
     1321 gaattcttat gggcagattt gccgttttaa ctcgccaaaa taatatcttc taccttctac
     1381 ccgtattact tctaagctat gcaatatttg atatttgttt ggtcagctac actcgcactc
     1441 gtgatggaag acatataagt caaggcggta gagaccattc cacacatcgt ctcctcacta
     1501 tgttgggctc agttaaaatc actgctataa ttgtttatgc tcttaatctt atgattgttc
     1561 tgactacgat tataattttt atcacaggaa atggagttct acttattgct actgctatta
     1621 tgtttacttt gtttttccta tttttaggac acaagcttga tcagatacca attgtcatac
     1681 ctatcaatca acaaaaaaca ggaaggtcat taaaatgaaa tttattcttt taggtacagg
     1741 gtccggaatg ccggaattgg gaaaaaatca aagcagttta tatgttaatg taaatgggaa
     1801 gcatcttctt tttgattgtg gtgaaggagt ttcccagcaa ttactaaaac atcatttaga
     1861 taataatgtt ttggatgcaa tctttatcag tcactatcat cctgaccata tttcagggtt
     1921 atttatgctt ttacagatgc tgaagataca aaaaagagaa aaaccactac aactttttct
     1981 gcctgaaaga cctgcagctc tggttgaaac gctgcaattc ctctatacct ttatccagaa
     2041 atttaccttc ccccttcata ttctggattg tgaagagagt gaattatatt atgaagaagt
     2101 taatgtggct ctgactgacc atttacttga atacgaagat ttagtgaatg agagtaaact
     2161 accgaatctt ctacattcct atgctttccg catttctggt cctgaaggct ctctcgctta
     2221 tacctctgat attaataaag ttgaaagcgt tcaattcctc atacgtaatt gccatacagt
     2281 tattattgat gcacagcatc cggaagctga ccagataata agactgcaat attctggtat
     2341 taaaagagtg ttattaacgc acggtatctc tcctacttta caggctttcc tttttgaaaa
     2401 cccaatagat atgtttgaat ttgctcaaga agatatcacc tacattatat gaaatataaa
     2461 tacattatct ttattctttt aagcttagtt ttattggtta tttctgcttg cgaaatcaat
     2521 caattgaaca gtgcagaaga ccactataaa aataagcgtt atgctgccgc tattcaggag
     2581 ctggataact atattcagac aggaaaaaat ggagctctta taacccgtgg tgaaattctc
     2641 agaagtcaat gctattatga attaggtcta agagctattg aactggaaaa ttgggatttg
     2701 agtataaagt ttcttaaact tgccaattca gaagaagcgg ataaggtttt agctgatgtc
     2761 tataaaaaac ttgctgataa agcattggag gaaggggaac tggaactttc attcaatctg
     2821 gtaaacacta ttctgcgaga aattcctcat tctgaactca ctgctgagat gctaagtaga
     2881 agaattagct tccttctgga tacctttata gattatgagc aagcctggca agattatatg
     2941 actctataca ataattaccc taataatgta tatgaggtgg ctgctcgtaa acagattgac
     3001 agaattatcc cctcacgtat aaattatgcc caacgtcttt actcttccgg ttactatagt
     3061 gatgccttaa atattctttt tgaactgcaa aaatatcccg ttggcgaccc gcaggctatt
     3121 aataaaatga tttgtgaagc ctatatggga caagcggaaa actcgttaca acagcaaaac
     3181 tatttagagg cagaacgact ctttcgtatt gctattcagt atgacccttc ccgcaaagta
     3241 gaggctgaaa agcgcataga acagatggct tcactctttg tgcaaaaagg tgatgaatta
     3301 gttagacaac gagattatga taatgcttta attcattatc aaaaagcctt tgatattata
     3361 ccagattatc ctcctgccaa acaggctatt gctcggttaa atactattaa agaaaatgtg
     3421 gctcgggcta atgaacttta tgctcaagca gaaaaagcag aacttgctgg taagtatagc
     3481 gaagctttaa aattgtatag gcaggcaaat agtctggata gtcgtccaga attgactact
     3541 aaaatttctc aaatgcaaaa cctaattgag gctcaagctg accctattgc ctttactaaa
     3601 cgcattatta atcagtataa aggcggaata ataaacactc gtatacaaaa acagaaacag
     3661 gaactgctaa agatgtttga tccctcagag gtacaagact ctggctggaa aattatgatt
     3721 acctccaatc ctttgaaata tgaagcacgt tatgacttaa taactccaac cgtatcctat
     3781 tattatgtgt ggttagtcaa tcttagagat ggtagtctga ctcctttaaa caaactttca
     3841 gaaaagacaa tggagtaatg atgcgaaagt ttatgctaat atctctttta ctatttctga
     3901 cggttatgct tactgctcag actcaatttg atattaatgt ctttgctgat accactaaat
     3961 atggatggca ggactggaga gatcgtcttc gctatcgtga agatttaatg catcgtcagc
     4021 aattactcca gatttacgaa atgcaatcca atccgcttcg ccctacagtc cttaaatcag
     4081 ttgtaattcc aggttgggga caattagcct gtaaagataa tctaaaaggt tctgttattt
     4141 taggcacgga agttctttct ctgggtgttt cactttactt ttatgataag agtaattatt
     4201 actataacaa atatatgcaa gctaatcaga ttgaacagat agaggattat tttgacaagg
     4261 ctcaggacca gcgtttttac tcattgctct ttatgggatt aggaggagtc atctgggctt
     4321 ataatatctt tgatgttatt cagaccacag aggattacaa tgctcaggtc tggcaagaga
     4381 ttttggaaaa atatagtcaa aaaaccgtta ccttgacacc tggtggaatc cgattacaat
     4441 tttaggatat taaaatgaaa ttcaaaataa tcttattgat agttattact cttttcattg
     4501 ggtcctgttt acagagaaat aatcctctgg accctgtggg caatcctaat gtaatagttc
     4561 cagatgaagt tcgtgatgtt tcttgcactg cttctcctcc tggagcaact cataaatatg
     4621 tgattattcg atggactgcc aataatccag aaaatacaga tggatacaaa gtttatcggg
     4681 ggttagctta ttcagctaat tacacctgtg ttgatacagt ttataccaat gaatgtaatc
     4741 acggttctaa agcctggcat actgttttac caggagatta ctggtacaga gtttcaggct
     4801 ataaagatat ttacgacatc aatactcatc aatatatagg aaggttagag ggcagactga
     4861 gtgaagcacg ttttgtgcgg gttcctcttt agataaccat ctatgcatac agactttaaa
     4921 caggagctga atgaacagca gctccaggtt gttttggaaa cagaaagacc tcttctggtt
     4981 ttggcaggag caggtagtgg aaaaactcgt tccttaattt atcgttgtgc ctggctgatc
     5041 aaagaaaaaa aagtgccacc taagaatatt cttatggtca ctttcaccaa taaagcggca
     5101 aaggaattgc aggaaagact gcaaaatatt ctcggttatt ctgtacaaaa cctgtgggtt
     5161 ggaacttttc accatatttg cactcgtatc ttgagaaatg aagcttcctt tcttcccttt
     5221 agacagaact tttctattta tgatgaaaat aaccagttat ccctattgaa aaagatatat
     5281 aaagaacata gtctggacag agagcgtttc cctctgcaac ctgtattaaa tcgcatcagc
     5341 agttataaaa acaggctcct tttgccagaa gatttaaatg agtataacct tgaaatctca
     5401 atggaaaact ataaacttga ccggacggtc ccaaaaatat atgaacttta ccagcagaat
     5461 ctcttgatga ataatgcctt agattttgat gacctgcttc tctatacggc aaaacttctc
     5521 caaaacaatg agttagtgcg gaaaaaatac cagcaacaat tccagtatgt aatgattgat
     5581 gagtatcagg ataccaatta tgctcaattt gaaatagtgc atcagattgc tgctgaacac
     5641 cagagaatat gtgtagtagg tgatgatgac caggctatct atagcttccg gggtgccact
     5701 atccgtaata tccttgaatt tgaaaaagac tatgctgatg ctatcgctat cagattggaa
     5761 caaaactata gaagcacgag ccatattctt gaactggcaa atgctataat cttaaacaat
     5821 cgtcatcgtc acagtaaaac tctctggtct gaactgggag aaggctacaa accagaactc
     5881 tttgtatatg aagatgatat ggaagaagca aacaagactg ctgacctgat tttacaattg
     5941 catgaagaag gtattccttt tcgggaaata gccattttat atcgcaccaa tgcacaatcc
     6001 agggtgtttg aatatgcctg tctgcaacgc aatataccat acaccattgt cggaaccgtt
     6061 catttctatc aaaggaaaga gataagagat ttactggctt atctcactgt gatatttaat
     6121 ccttcggata ataatagcct cttaagaatt ataaatgaac ctcccagagg tattggctct
     6181 gtttctataa atagattgat ggaatttgct tctcaaaatc atctatcact ttatcaagca
     6241 atacaaaatg tccaagcaat tcaatccatt caaccaggaa tcaaaaagaa aatcgctgag
     6301 tttgcggaaa aactggaaga ttggcacaga aaagcagaga acacacctgt gctggattta
     6361 gttaaagaag tgattgattc gttggacctt attaatcact ataaaaacag tcaggaccca
     6421 gcggaaatct cacgagtgga aaatctgatt gaatttgtaa gttcagttag tgagttctcc
     6481 gaaagatgga tagaagaata tgataaatct ccgctcctgg aagatttttt accctttgtg
     6541 gctttacaaa ctgatataga cagggttaat gaagctgcag atacagtgta tctgatgacc
     6601 ctgcataatg ctaagggctt agaatttgaa tgcgtgttca taaccggtct ggaacaggaa
     6661 cttctgcctc atttaagaag tatgaacagt aaatatagca tagaagaaga acgaagactg
     6721 ctttatgttg gtgtaaccag agcaaaaagt agattgtttc ttagttatgc caaatatcgc
     6781 agaaaatatg acagctttta ttttactgaa ccaagtatct ttcttcagga acttgaccca
     6841 aatctttttg caggttataa tgatgctgtc acttttaagc ctaaaccagc taacaaggtt
     6901 tatgctaagc ccaaaacttt aaataataac caattttata taggacaaaa agtctggcat
     6961 acagaatatg gaccggggtt agtattaaat gttgatggaa atggtcctga agcaagatta
     7021 actatctctt tctcatctgg gaaacttgct aagattatcg ccagctatgt tagtcccgaa
     7081 ccaatttaat gactccaatg ctttatgagg taattcttcc ttatctaaat gcttctcctt
     7141 tcttccagaa actttctaaa ttattcggaa caaagcagca ttatcagatt catcatctca
     7201 atcagagtgc cagagctctg gtaacagcgc atttatggaa agaaacagga aagaatatcc
     7261 tgattgtttc tcaggatgat ttaatggctg aagacctctg ggatgacctt tgcacgctta
     7321 ttggacagga aaatgctctc tatcttcctg agtatgaagt gctaccttat gaggaacgtt
     7381 cacctcacta ttccattcgt gcgacaagaa tgatgtgtat tcatcatatc cttcagggtg
     7441 caccttctgt ttacagtatt tctattcgtt ctttgctacg tatgttgcca cctctggaaa
     7501 atattgctaa gcatatcaag gaattgcgtc ctcaaatgga gtattctcct gataaactga
     7561 tgcaagactt ggtctggatg ggttatgatg ttcaatatca agtaactaaa gtttttcaag
     7621 tggctcgcag aggaggaatc attgatattt tcagtcctcc tcaattaaaa cctgtcagga
     7681 ttgaattctt tggtgatgaa attgtctcta tgcgctattt ttcacctaat actcaacgtt
     7741 ctattcctgg agatgtggaa tcctacactt tacttccatc ccgagaattt tcgctggatg
     7801 atattagtcc tcattctatt cttttgccac aaataaagaa tactggcttt tatgaaggta
     7861 ttgaaaatca atattgtctt ttgctggata aacttagcac tttcgctgac tactttgaag
     7921 ctcaaaatcg cttgattctt tttagtaatt tcccctatat tcaagaagag ctaactcgct
     7981 taactgaaca ggtacaattg gaatatcatc gccagttaaa atatactacc aaagcaaaat
     8041 tgagtcctcc tgaaggaatt atcgctggtg aagaaaagtt aatggaactt tgtcagccag
     8101 aatccagttt gtttttatct caaagtgaat ttatcctgcc cttccctact gaaaacatta
     8161 aagctcccat agagccacaa ccttctttca atggtaatct tacacttctt gcttctgctc
     8221 tcaatcaaat ggaaaaagaa cattttaatc ctatccttct ttttgataat atcagtcaga
     8281 gtaaaagaat gcaggagctt ctggaagatt ataattgtag ccctcaaagt tatattggtg
     8341 tcttgcatga aggttttact attactgatg caggtttagc tctttggacc gaccatgaga
     8401 tttttaaccg ttataaaagg aaacgctaca ctccaagatt ccctccagga gaagagattg
     8461 tagattatca aagcttaaaa cctggagatt atattgtcca tattgaccat ggaatcggag
     8521 tttttgaagg attacaaatc ataaaattgg atggacagga caccgaatgt ttggtcttgc
     8581 gttatgctgg agatgatagg gtttatgttc ccacttatca attggattta gttactaaat
     8641 atatagcgga agaaggtgta gctccaactt taagtcgttt aggttcaact aaatggcaac
     8701 acacgaaaag aaaagcagct gaacaaattg aacttatcgc tgcagattta gtaaaactat
     8761 acgctgaacg aagttcacgc ataggtatag ctcatcaaac agatacaccc tggcaaaaag
     8821 agctggaaga atcttttatt tatgaagaga cagaagacca gctcaggtct attgaagaga
     8881 ttaaagcaga tatggaaagt cctgtcccga tggaaagatt attatgtggt gatgttggat
     8941 ttggaaagac tgaagttgct atccgagcag cttttaaagc ggtcacttct ggttatcagg
     9001 tagctgtgct tgttccaacc accattcttg cagaccagca tttccgcgtt tttaaagaaa
     9061 gactggctca atatccagtg cggatagcta tgtttagtcg tttcagtagc aacagtcaga
     9121 tagcagaaga tttagctgat ctggctctgg gtaaggtgga cattgctatt ggaactcatc
     9181 gtcttctttc ccgagatata catttcaaga atcttggtct actaataatt gatgaagaac
     9241 atcgctttgg ggtgcgccat aaagaaagat tgcggaaatt gcaatccaat gtagatacac
     9301 tttatatgag tgcaacaccc attccaagaa ctatgaatat ggctctggca aagttgaaag
     9361 aaatatccct gatgcaaact tctcctaagg aaagactgcc cattagaacc attatcactt
     9421 taagagaaga tgaagttatc cgggaagcaa tccaaagaga aattgaccgt ggtggacagg
     9481 tatttttcat ccataatcgg attcagacca tagagacagt ggctaaacaa cttaaagagc
     9541 ttatgcctgg agttaaattt gccgtcactc atgcccaaat gcctgaaaag cagctggaag
     9601 atattatggg agctttcatc aatcgggaat atcaggtcct catttctacc accattatag
     9661 aaaatggtat tgatataccc aatgccaata ccatcattat agatagagcg gacacctttg
     9721 gtttagctca actttatcag atgagaggaa gagtcgggcg ttccaatcgt cgtgcctatg
     9781 cttatctttt aatttccaga aagaccactc cagaagctcg taaacgttta gagactctaa
     9841 ctcaatatga ttatttagga gcaggtttcc aggtggcttt gcgagacctg gaacttagag
     9901 gtgcgggaac tattttagga accaaacaga gcggtatcat tcaatctata ggattcaact
     9961 tttacaataa tatgctggaa aatgctattg ctgccgttca aaagggagag agtatagctg
    10021 agcagattga gactcctaag cctcagcgaa aactctttac tgaaatagat ttatatttcc
    10081 ctcctgatta cataagtgat gacgaacaac gcctaactat ctatcgtaga ttaagtgaat
    10141 tagatagctt acaggatata gaagatctgg agctggaatt gaaagaccgc tttggacccc
    10201 ttccagaaaa ggcttcctgg ttgctgatgt attttaaact caatatactg gcaaaacagg
    10261 cagatttaca aaattgctat gtccggcaca atgttctcac tatggaattc aaacttgaaa
    10321 atcttcctcc acgggataaa gtgctctctt ttactgccaa agctccgggt gttcttagaa
    10381 ttgatgcctc taagggtctg aaaataacta tctcgctgga tgagaaaaag aaatatcaag
    10441 accagtttgc cgatgcttta gaactactcc agttgtatat taactcctga ttttatctat
    10501 tcttatataa tcttctttca aaagagctcg gctctttcct cttttcatag aatatattta
    10561 tcttctgatt acctatatca ttagctgaaa caacatagtc tcgtaaatct ttctgctcta
    10621 ttttctcagg ttgatatgaa tacagctgat gattaaaacc agctttaatg ctatcttcag
    10681 ctaattgatt ccagtagttc atatctccac cagccggagg aatccatccc agttgcaaga
    10741 atagatggag tatctgatta atcaggttgg atttagctgg attataataa tgagtgatat
    10801 atccttgatt agccaaagcc gtgccatcta tctctaaata agtaggttgg ggtaaaatca
    10861 aatctgcctc tgaggaagaa tcaataaaag gaaggatttc cacactaaac ctcgtattag
    10921 aggatttctt actcttcgtt ccgtaactaa gtattaattc agcatcaaca ttatctttcc
    10981 attcagaatt taaaagctgt aatccataat aatttggata atctgaactt tccagtatat
    11041 ttcccaatcc tgaatccagg gacactgcca gattcaatat ctcatagata tctttctcgc
    11101 ttatctgatt gatattatat ataaataact gatttttatt accctgcaag gcagaaaggg
    11161 aagaatagac tttatccgca aaatgcagag ccgtggagtt ttgacatagg acacttacca
    11221 atctcttgcc ttttttctgt tgcaaccgta agagagtgcg taagttctgg ctaatttctc
    11281 ctattaccac aaattcggaa aagtcttcca atatcttgta aggttcaagg tttggtttaa
    11341 gcgggagata ttggctgcta aagaaatgat aatcttgaga agcataaagc acagagccag
    11401 tattttttgc tgcttttccc agtataagca tctcttccag agttattaaa ggagagatgg
    11461 caaaagcata actggaaagc tctttcactt tttcttgcag caaggaatag gtttcttgcc
    11521 agtttatcgg ttcccatttc ccttttcttt taactacagg cgattttaat ctttcttcag
    11581 tatagaaagc ttgccaacca aatcttcctt taaaacatag attgcgtcca ttgaatccag
    11641 gagctttttc atcagttcct attaaaacag gcactcctga ttgactttcg cactggatac
    11701 aacagcctgt accgcaaata ccacaatttt gttttgtttt ttctttttcc aatggattca
    11761 atttataatt aatgtttctt tctaccaaag ctcctacagg acatacagca atacatttac
    11821 cacagctttc acaggaagtc ttagttaacg attctccaaa ttcaggagct ataagagtag
    11881 aaaaaccacg ataaatatat ccaagaacac ctggaccctg aatttcggaa cagattcgca
    11941 cacatctgcc acaatcaatg catttattgg ggtcgcgcaa gatataagga tgagaataat
    12001 ctatacaata tttattaata ctaccaggat aagacagagc ttccactttt aaatcagtgc
    12061 aatattctct caatttacag gtttcattaa cctgacatcc acattccaga cagcgttctg
    12121 cctcaaattt tgccgagatt tcattaaatc caagttcaac ttcttcaaaa gagttttttg
    12181 ctttatctat cggtatatta ggcattttct cacgggctat tttttcatac ttcctgtatt
    12241 ctttaggaga gatgtcctga atcttataac ccttcttgga gtcaaagcgg aaggtatctt
    12301 tctctatttt ttgaccttta aggaaacggt ctatagcttc agcagcaaaa taaccatctg
    12361 caatagcttc tatggcagtt gcaggacctc tgcgaaaatc tcctcctgca aatacatttg
    12421 ccagacctgt atacatagtt ttttcatcta ctatagcggt ttgccagcga cttataggta
    12481 aaatctgtcc ttggataaaa ttatcttctt gagcaaaaat ctctacttct ggaatttgtg
    12541 agatagcagc aatgatagaa tcataggatt ccagaaagta ttctcccgta ggaacaggtc
    12601 ttcttcttcc actggaatcg ggttctccca gttgcatttt ctcaatctta acttgttgca
    12661 atttgccatc actgcctata tattccaccg gattacttag ataatgaaag attacccctt
    12721 ctttttcagc agcttctatt tcttctggtt cagctggcat ttcctgacga gttcgtctat
    12781 aaataacggt tacctcacaa cccagacgaa tagctgtcct ggcacaatca atagctgtgt
    12841 ttccacctcc tactacagca acctttttac ctaaatatgg agtttgcccc aagctgtgag
    12901 ctttcaagaa atccactcct aaaaaacaac cttgcaggtc tgaacccttg accggaaaag
    12961 gaaccgcttt ttgtgcacca atagctaagt aaactgcatc ataagcttga gagagctcat
    13021 ttaattttag gtcttgccca agtttgatat tatactcaat ctccattcca ttggaacaca
    13081 tcaattcaat ttctctatct aatatgtctt tgggcaacct gtattctgga attccataac
    13141 gaagccagcc accagcttca gcagcagatt caaaaacctt aacctcatat ccctgattag
    13201 aaagatagta tccacaggtg agaccggaag gacctgcacc tatgatagcc acctttttgc
    13261 cagtggattc ctttttctcc ggaacataac tccatacatc attcaaatct tcatctgctg
    13321 caaacctttt taattgacga atggctatag actcatccac aatctgacgc cgacattctt
    13381 tttcacaaaa agcaggacag attcttccaa tagaaagagg cataggtaag gtttttttga
    13441 taacttttac cgcatcatga tactgaccat tagcaatcaa ggaaacatag ctttgaacat
    13501 ccaccagagc aggacaggca agtttgcagg gagcttcaca atccgcatag tgattagaga
    13561 tgagtagctc cagcgccatt ttgcgggcat tgctaatttc tgcactatct gttattatct
    13621 ccattccagg ggcgatagtt gtaccacaag aagttacaaa tccttttctt cctttgactt
    13681 caactaagca gacccagcaa gaaccatatg gatttaactc ttcatcatca caaagagttg
    13741 ggattttaat gccatttctt tttgccagtt ccagaatagt aattccggct tcactttcta
    13801 ttttctttcc attaagtatc acttcaatca tttttaactc ctactctctg gttacagcac
    13861 caaatttaca ggctttataa caattcccac aatgggtaca tctactttga tctataatat
    13921 gaacttgtct caaacttcca gagatacagg aaacaggaca cattctggca caaagagtgc
    13981 aaccaataca tttttcagga aggatgtaat aatgcagaag agcagtacaa actccagcgg
    14041 gacatctttt atctacaata tgtgctaaat attcatcctt aaagtatcgt aaagtggtga
    14101 gaacaggatt aggtgctgtt tgtcccaatc cacaaagaga acccttacta atatttaacg
    14161 ccaattcttc cagtctatcc aggtcttcaa gttctccttt gccctctgtt attctgttta
    14221 atatttccag cattcttctt gttccaatcc ggcagaaagt gcattttcca caggattcat
    14281 tctgagtaaa attgagaaaa tagcgcgcta catccaccat acaggttcca gaatccataa
    14341 ctaccattcc accagagccc ataatagcac cagtcgctgt gatactatca taatctacag
    14401 ggatatcaag caagtcagca ggaatacatc ctccggaagg tccacccaat tgcactgctt
    14461 taaacggttt ttccgttttc ataccaccac cgactttata gataatgtct cgtaaggtta
    14521 ttcccatggg cacttcaatt aatccgctac cagcaatttt cccggctaaa gcaaaaacct
    14581 ttgttccaga agatttttcc atcccaattg ccttaaaagc ttctccacca ttttttataa
    14641 tccagggaat atttgcccag gtctcaacat tattgatatt tgtaggttta ccccagagac
    14701 ctgattcagc tggataaggt ggacggatgg aaggcattcc gcgtttacct tcaatagatt
    14761 gcataagtgc agtttcttca ccacaaacaa atgctccagc accttctttg atagaaagtt
    14821 caaaactgaa atctgtcccc agtatattat cccctaaata attcttttcc cgggcagaaa
    14881 caattgccat ctttagatgt tcaattgcca gaggatattc tgctctacaa tagatgtatc
    14941 ctttatgtgc tccaatagca taggcaccaa taatcatacc ttccagaact ctatgaggat
    15001 ccccttccaa aacactacga tccataaagg caccaggatc accttcatcc gcattacaga
    15061 taatatactt tatatcactc ttagctgcag cagcaaagga ccatttcaga ccagttggaa
    15121 atcctgcacc acctcttcct cttaaacctg atatcttgac ttcattaatt atctcttggg
    15181 gacttaaagt aagggctttt ctcaaagctt gatagcctcc agaagcctca taatcttcaa
    15241 tggataaggg atcaattctt ccacagtttt gcagaacaat cctctgttga taggcaaata
    15301 aatcgttaca aggagcttca actttatcgg ataatatgat attagaagaa ggaattatgt
    15361 cctttctata ttcttcaata agatttatta cattctctgc attgacttta ccaagtgtga
    15421 tttcacctaa ttctgagcca cttaaggata caatcggttc ttcaaaacac ataccaatgc
    15481 atccggtctt ttttaatata taagcccctt cattttgctc taaatactct tttagcttat
    15541 cataaacttc cattgcacca gcagctcttc cacaacttgc caaaccaact tttactataa
    15601 tctcactcat atattatcac ctatttctta aaccgactca aaatccccgg tatagcattg
    15661 ggggtgagtt tcccaaaagt ttgctggtct atcattatca ctggagaaag agagcaacag
    15721 cccagacatg caacttccat aacggtaaat ttaccatctg ctgtagtttg ttctccttct
    15781 tccaatttta aataatccac tacagcttct tttatactat ccgcatctgc cacatgacaa
    15841 gcagtgcctt tacaaatttt tattatatgt tcaccttggg gttccagacg aaacatcgca
    15901 tagaaagtta ccactccata gatttcagca ataggaatat gcatcttttc tgagatatag
    15961 cgtatatccg atttggataa atatcctttt tgacgctgaa cagcctgtaa tattggaatt
    16021 aaattacctt ttttgccctc atattcctgc aatatttcat ccacagaaat gaagtcttgc
    16081 tccattgcaa ctcctttaac tatattcctg agtcctttca gataacaatt ctgaacccca
    16141 tttttgagac caatctatgg actttaactc tttgtcaaat aaaaagcata gaaccaaggt
    16201 ataaagctat tctcatcctt tcaagctctt caagcacttt ataggcaatt taatataatt
    16261 ttttcatcat aagattagaa ttagatgata aataaggaat taaaaaatat atttgttgta
    16321 ttcaatgatt tacaacttta tagtaattat atataatttt tatggagctg gataaattta
    16381 tagtttatga cagctacttt ggagctagat attataagaa tatttttcat ccatagtaac
    16441 ggaagatatc tgcatctttt tgtccagctt atgtccacct catgtccagc ttatgtccag
    16501 cttatgtcca actcatgtcc gaaattggac ataagctcaa gaaaagcagg caatcaagta
    16561 ttcatcaaaa gataaaaaat attcggaagg tagatttgac ctcataaaga acttgccaat
    16621 atagagctat gctctaaaag gtaaaaaaat aagaattata tattaaggaa atagattaaa
    16681 tgccagaact tccagaagtg caaaccattt taaatgaatt agaaaaagaa ctaaaagata
    16741 aacagataga gggattggat tgttatcatt ctcgcacttt attgtgggac aaagaattaa
    16801 ccgccactcc ctttcccgca aaaattatag gtttggaaag gcgaggaaaa tttatcctta
    16861 tccatctaac aaatgagaac acgattatta ttcatctcgg aatgactgga aaattggtgc
    16921 gggacaatac catagctaac cattctgaac acgaaagagc ctgttttcgc ttatcggaaa
    16981 aagaaaaaat acactttata gatatccgca ccttcggaaa aattgtcctt tgtccaacta
    17041 aaaatatccc tgcctacttg cctgaacttg gtgttgaacc actatctgag gattttaatc
    17101 tttcttattt gcaaaaagct cttaaaggtc ggaaagcacc tataaaaaat atcttaatgg
    17161 accaaaaaat cattgctgga ctgggaaata tctatgctaa tgagattctt tatcgggcta
    17221 aaatcaatcc tgaaactcct gcttcctctc tttcttccca aaagctttcc aagcttataa
    17281 aaacaactaa gactgtcctt aacgaagcca tagagaaaaa tggaacctcc atatcggatt
    17341 atcggaggat tgatgataaa aaaggtgagt tccagaattt tttacaaatc tatcaaaaaa
    17401 ccacctgtcc caagggacat cctgtccaga aaataaaaca aggtggacga agcacttatt
    17461 tttgtcctgt ttgccagaaa tgaagtttat ttttgcaaac ctgtgttaaa aagtgtatac
    17521 tataatagta ggtatttata tcgtataaaa gattccaaaa aaggagattc aaaatgaaag
    17581 cctataaatt gaaagataat atctattggg ttggagcaat agattggaat ttacgcaatt
    17641 ttcacggtta tatcaccccc agaggttcca cttataatgc ttatcttata atagatgaga
    17701 aggttacgct gatagataat gttaagtact acctttttga tgagatgctc gctcggataa
    17761 gtgatattat tgatccgcat aaaattgata ttatcgtcca gaatcatatt gaaatggacc
    17821 attccagtgg tcttcctcaa cttatgaaaa ttattcctga agcaaagatt tacaccaatg
    17881 ccagcgctat caaaggtctg aaattgcatt atcatcagga ttggaatttt caggaaataa
    17941 aaagtggtga tagtataaat attgggaaaa atgaccttac ttttctaact acacctatgg
    18001 ttcattggcc tgataatcag gtaacctatt gtccacagga aaaactactt ttttctaatg
    18061 atgcatttgg acagcatata gcttctaatg aaaggtttga aagtgatttt cctctggata
    18121 tagtgctgga agaagctaaa aaatattatg ccaatattgt ccttccttat tccagacaag
    18181 tgcaaaaagt gatggaagct gtttcctctc ttgaaataaa tatgatagct cccagtcacg
    18241 gtttaatctg gacagaacat attcctgaaa tattaaatgc ttataacctc tggtcaaata
    18301 acatcacaga taaccaaaaa gccctggtaa tttacgacac tatgtggaaa tctactgaaa
    18361 tcattgctta tgctatagct caagcttttg aaaataaagg cattaaagct aagatacgaa
    18421 acctacaatt aactcatatc tcggatatta tgacagaggt tatggatgcc aaatatatct
    18481 gcgtcggctc tcctactatc aatagctcta ttcttccaac cgtcgctgct tttatgagct
    18541 atctgcaatg tctggctcca aaagaaagaa ttggtctcgc tttcggttct tatggttgga
    18601 gtggacaaag cgttcctatg ctccataaaa tgctgggaga tgatagtgga tgcggtttta
    18661 aaatgttaga acctgtaaaa gttcaattta ttccagatga tgagattctt aatcaaatca
    18721 gagagaattt agagcgacaa ctggataata tgtgatgtaa tccactatct ctatatatat
    18781 tatgaatact taaatccagg tttattatta acttgtactg ggtggttaaa aaaatattga
    18841 cgcaagggct aagaatatta ttttagatta taggataatt caagggtaag tggataatga
    18901 agaaagaaag caagaaaaaa ataatcggtc tttttgatgg gattgatgac attattgtta
    18961 atgatattaa acagcaatta aggaccgtca aatttaaaaa aggcgataag atactgcgag
    19021 aaaaggaaga aggctcctgt ctatatgtaa ttctcaaagg caaaatagaa attgaaaagg
    19081 aggttgaaca ttctgaacct cctaccgctc ctttaaccgt tatggaagaa ggagaattct
    19141 ttggagaaat gagtcttcta aatgaagaac ctcgcagtgc aaatgccatt gctctggaag
    19201 acactgaatt acttgaaatt cctgaagaag aattccagag actttgcttc tcctatccta
    19261 atattatgtt aaatctggta cgagctcttt ctgaaagact cagattaaca aatgaaagat
    19321 ttgctggtgc tcttagtgaa atgatacaga aaaaccgttt aacagctata ggaaaagcaa
    19381 ccagcaagat aatacacgat atcaaatctc ctctcaccat tattagttta aatgctcagt
    19441 taatagagac ccttttcccg gaagcagaag aattgactca aggcataatt aaacaggcta
    19501 aattgataga tgagctttta caggaaacac tggattatat taaaggcaat cccaatcatc
    19561 ttataattca gaaagtagat atggttaaat ttttaaaaga tatagaagat acctatggtc
    19621 cttcactaaa aagcagaaat attgaattgg tcatagaaaa taagtgtaac gaaccggttt
    19681 attttgatga ggaacgcatc cgacgtacta ttatcaacct cattcgtaat agttcagaag
    19741 ccatcagtac taagggcacg ataaaaataa tagctaattt atcctctaac tggttacaga
    19801 taagtgtgat agatgatgga ccgggtgtcc ctcctgctat tgtagatgac ttatttaaac
    19861 cctttataag ctataataaa cctactggca caggtctggg gctttctatc tgtcaaaaac
    19921 ttgttcaaga acataaaggt agaatagaat acacaccagt gcaacctcac ggtgctcgtt
    19981 ttgatattcg cctcccacaa caacataatt tatagagaca aaaacttttc agagaaacct
    20041 aatttttaac atactataaa ggtaattcat agaatttaaa taaaatcttg tcaaaaaaag
    20101 cattaataaa aagatggaaa aaagaaatca atttggatgg tgaaagatgt caaaagtatg
    20161 tgatatatgt ggaaaaaagg ctcaggttgg taatcttcgc agtcatgcct tgaatgcaac
    20221 taagcgccgt ttttatccta atttacatct tgtccgggct gtcgttgatg gcgaggtcaa
    20281 gaaaataaga gtttgttctt cctgtcttaa aacaaataaa gtccataagg ttgtttaata
    20341 agattaacca tactgcacta cagttaaata aacaatgcca attgtgctac atcgggaata
    20401 ggactatcct gttcccgata tttatctaag agaaaatcag gagaatagat ggatattcaa
    20461 gagttaaact ttaaagtggc aacgagttca gcgctacttg ataatttaag aactgagatt
    20521 gccaaagtaa tcgtaggaca ggatgctata atcaatcgtt tattaattgg acttttagct
    20581 aatggtcata ttctattaga gggagttccc ggtctggcaa agacactaat aataagcact
    20641 ttagctcagg cttttgattg cgtttttaaa cgaattcaat tcacacctga cctgcttcct
    20701 gcagatataa ctggcacttt aatatacaat caaaaaacgg gtgaattcac tactaaaaaa
    20761 ggacccatct ttgctaattt cattcttgca gatgagataa atcgtgctcc tgccaaagtt
    20821 caatctgctt tactggaagc tatgcaagaa cgacaggtga ctattggaga taccacctac
    20881 cctctccctc gtcctttttt cgttatggca acacaaaatc ccatagaaca ggaggggact
    20941 tatcctttgc ctgaagctca ggttgaccgc tttttaatga agctcaagat taaatacccc
    21001 actctggaag aagaaaaggt aatccttgaa aggatggtag tagaacgaga acttagagtt
    21061 aatccggtgc ttaaaccttc agatttagat tatatgcgag agatattagc tgatttgtat
    21121 atggaagaaa ggttaaagca ttatatcctc aatctggtac aggcaacacg ttatccccaa
    21181 cgctatcctc gtatatcatc attaactggc ttgatacaat atggagcttc cccgagagct
    21241 actatctttc ttgctcgtgc tgctaaagct aatgcctatc tggaacaccg agcttatgtt
    21301 atccctgatg atataaaagc cataggcaaa gatgtcttaa gacatcgtat tatcctttcc
    21361 tatgaagctg aagcagaaca ggttagtaca gaagatataa tcacccgcat atttgaagag
    21421 atagaagtcc cctaaaggat tcaatctatg tcttccccca cacctgcaga gattcttgct
    21481 cggattggga aactggaaat ccgaaccaag aatctggtta atgaaatctt tagtggagaa
    21541 tatcgttcca gctttaaagg gcagggtctg gaatttgctg aagtccgtga atatcaagct
    21601 ggagataatt atcgggatat tgattggaat gtttcagctc gtttaggtgc accctatgtt
    21661 aaaaaatata ctgaaaccag ggagctaaat gtcttcttta tcgttgatgt atcagcttct
    21721 atgcaatttg gtacggtgca accaaaaaaa caagaacggg tagcagaaat cattgctcta
    21781 ctttcctttt ccgctctttc caataatgat aaagtagggc tgataatgtt ttccgaccag
    21841 ctggaaaagt atcttcctgc tcgcaaagga agcaataaag ccttggagat tttaagagat
    21901 gtgctctatc ttgaaccaga acataaaaat acttccttac tggaagccct aacctttgcc
    21961 aatcatattc tcaaaaaacg gtctgtggtt tttatccttt cagattttat ggatagtggc
    22021 tataataaac ctctggaaat cctggcacaa aaacatgatg taatagccat ccagattctg
    22081 gatgaggcag aattaaaatt acctgatgca ggagtcttaa atctttacga cccggaaaca
    22141 ggagacactc tttattgtaa tacttctgcc acatttatcc aaaaagcata tgaggcaaga
    22201 gtttctaaac aacaggaaga cctcactaaa gacttggcta aaatgaaggt tgattacata
    22261 atgctaagaa gtagggattc gcatatcaag gctcttagaa ctttctttga acaaaggaga
    22321 agaagattaa gagaaggcag atgaaaaaaa tcctaccctt agctctaata ttgcttttta
    22381 taagctatct ttccgctgtt actatccaac aaaaattggt taatgatgac aacctgaatg
    22441 taggagaccg ttttcaacta caaatctacg ctgatgttcc tttaaaaagt gtagttgttc
    22501 cggacacttt aacttccttt aaaatcattg aagtagaaaa aaagaaagag ccttccggaa
    22561 aaacctggtt acagctcact attgttccta tactaactgg ttcactgagt ttccctaaat
    22621 tgcaagttat cccggaaatt tcagatgggc aaacttattt cacagatgcc ttccgggtta
    22681 tagttattcc cgttagagct gaaaatgata ccaccctggt tgatatcaaa cctttagcta
    22741 aatacccact gcaattacct atctgggtat actatctatt aattgcactc atcattgtgg
    22801 gaattatcta cttgattcta aatagaagaa acagaggcgg aaaagattcc acagtaaagg
    22861 aaaccattcc atcttctact tctctggacc ctccctggaa aatagcttta aaacaattgg
    22921 atgaactttt gtcccagaat ttaatccaga aaggtgaata tatccgtcat cactatgagc
    22981 tttccttaat tttacgacaa tttttagaac ggaaatatcg tttcccagct gtggaaatga
    23041 ccacttcaga aatacaacaa gttgccgaac gcttatatat tgccagcagt caagaagtcc
    23101 tgaatttcct gaaatactgt gatagagtta aatttgccaa atacattccc tccccagaag
    23161 aaatagagga atatgagaac tggttgaggt cctggttgca atcttttgaa ttggtagaag
    23221 cacaacaaaa gcttgctcat ggaggaattg agcatgctta gttttgctcg accctggtgg
    23281 ttaatagctc taattatagt acctttgttc ctctggtatc aatttaaaat ccagaaacca
    23341 cataaactgc gattaccatt tacgcgtttg cctcttctta aacagataaa aggagaaaac
    23401 aagttctgga aatacctttt ccctgcttta agaagtttaa tctttgtatg tttaattctg
    23461 gctatagctc aaccccgctg gggttatgga acacgagata tcagtcaaaa aggtgtagat
    23521 attgttcttg ctatagatat cagtggctct atgctggcta tggattttgc tcctcaaaat
    23581 agattaggag cagcagtcaa tgtggcaaaa gacttcatta aaaaaagacc taatgaccgt
    23641 tttggtttag tagcattttc agaatatgct ctaacccaga gtcctttaac ttttgaccat
    23701 gaagctattt tagaacaact tgaccgcctt caggtcaata aagaagcatc ttttaccgct
    23761 attggagtcg gtcttgctaa agcagtagcc agacttaaag acagcaccgc aaaaagtaaa
    23821 gttatcatcc taataaccga tggcgttagt aacacaggag aaatagaccc cctaacggca
    23881 gctaaaatgg caaaagctta tggaataaaa gtctatccta taggagtggg aacaaatggc
    23941 ttggtagatt accctattga ggacccttta cttggtactt actatagaaa aatgcaagta
    24001 gaactggata tgaatactct taatgcagtt gctgagatta caggaactgg aaaagctgca
    24061 caagctggaa ataaagcaca attacaacaa attatggata agatagataa gctggaaaag
    24121 accacttacc agattcaaat tcattatata tggtcagaaa agtttatgcc tctactctgg
    24181 attgccttta tcttgctgac catagaactg cttctgcatt tgatagttat gcctattctt
    24241 ccggagtaaa aaatgaatct atattatcct ggaaatatct ggttactact tattatcatt
    24301 cctttgatag tatctctatt tttctggaat aggcgtgaaa aaaagcgctt ctctcgcttt
    24361 gctaatcctt ctttctatcc tgaatatctt tctaatcgct cccagttttt gcactttttc
    24421 aaatatatat taattatctt ggctttagcc tttattattt ttgctttgct tcgtccccaa
    24481 tgggattatg aagagcgaga atacagtttc aatggacttg atattatggt ctgtctggat
    24541 gtttccaaaa gtatggatgc acaggatatt actcccagta gactgcttag agccaagatg
    24601 caaatagatt ctttgataga taagcttaaa ggagatcgca tcggcattat tgcctttgca
    24661 ggagtgccta ctctggaatg tccacttact gatgattatt cgtgtgttcg cttggtgcta
    24721 aatagtatta atactgacaa tgtagtttcc tatgggactg atattggtgc agcattagcg
    24781 ctggcaagca ggtcctttca atcagctggt ggaagtaata ttctcctttt aataaccgat
    24841 ggagaagacc tcgcaggaag cgctattctt caagctaaaa gattaagctc acaaggagtt
    24901 aaaatctatg ttttgggtgt gggaagcgaa gaaggcactt ttgttagaga tgaaaagact
    24961 ggtcagcaag catttacaaa gcttgatact aagacattac aggcaattgc atccataggt
    25021 aaggggaaat atttttccgt tagaccgggg caaggagaat tagaccccat ccttcagaac
    25081 atttatgcca gcgaatctgg tagggaaaga agtagaaact tctccatttt aaaagaccag
    25141 tatgccatac ctgccattat tgctataatt attttattgg tggaaagtct tcttctacct
    25201 ctttctcgta aaagggaaaa tgtatgaaag ataaaaaact gctcttctac aagcttatcc
    25261 ttattctggt tatacttatt ctgttattct taatatatac tttattattt aatcagaaat
    25321 cacttaaaca cagtagagca agtgaattat ataaaaaagg acagtttgaa aaggcagcta
    25381 aaatctggga aaaggaattg gatagcaaag ataaggatta ccttccggaa agcagtttgg
    25441 gtaaagcttt ataccagcaa ggtaaatatg aagaaagcat aaaaaaacta caggatgctt
    25501 tgaaagagaa tgataaagac cctggtttac attatgattt gggtaatgct tacttacgga
    25561 ataaccaaat agataatgca ttacaagctt atctaaatgc tatgcttctt gacccagatg
    25621 atgaaaatgc taaagcaaat tatgaacttg cacttaaaaa gaaaaatgct attctcctcc
    25681 aagctggtat aggcgaaaac aataaagacc aaaatcagca aaatcttcct gatcaatctt
    25741 tgcgtgaaga aatggaaaat agattaaatg cacttgacca aaaagaagcc agagatagaa
    25801 tggcaaagaa tcctccacct tataagggga aaggagaaaa gtggtggtaa aaaagtttgc
    25861 ctgtgctcta ttgatagtgc tctctctgat gggcatacta aaagctgaat cagtaacggt
    25921 aaatgcttat ttaaaagaag atgttattac cttatctgat cagctacaac tggtactgga
    25981 aataaccagt gataaacaag taaaagttat tgctccacca gctccccaaa taccaggatt
    26041 ctcttttcgt aatgtagtag gttcaactta ccttgattac tcccttgtta ataaaaattt
    26101 attcactaaa tacatacaaa cttttaccta tatttatatc cctcaaaaaa ctggcacctt
    26161 cactattcct tcctttaaag ttaaagttga aaagaaggaa tattatacgc ctcctttgac
    26221 tgttcaagtc aaagaatctt cacctccacc tcagaaacaa tataatcttt cacctttctt
    26281 aaatcctttt ggagatgaat tgttcaatcc caatctcata gaaggtgaag tatttttact
    26341 ctgtatccca gaaactgatc ttgcttatgt tggcgaaccc gtgataattt cctattatct
    26401 ttacacggaa caagcagttg aagctttcta tgctgaatcc gaaaaagatt ttgagggtta
    26461 cggcaaagca gtcttccttc agccttcctc acttaaatat gaatcttctg tctataatag
    26521 caaatcctat aaacgtgctc ttataaagca ggatgttatt ttccctcata aagctggaag
    26581 actacagatg ccaattctca caggacaggc acaattcctg ggatttttaa ataaaactat
    26641 cagttccaaa ccttgctgga taaatgtaaa acctttaccc tcaggagctc ctgctggttt
    26701 tacaggcgca gtaggaaatt tcaccgtttc ccagagtgtc tcttctacta agttaaacct
    26761 tggtgatgct ttaaccttca ctattaatat ctcaggaaga ggaaatttta gccagtttac
    26821 cgcagctcct ttccctaatg tagataaatt tcaggtttct gcaccctata ttcaggacaa
    26881 gctggctaat gtgattgaag gaactcgttt tattcattat actttgcttc cacgagaaac
    26941 aggggaattg actatccctg gatacacctt tagctggttt gatacaactt ctggagttta
    27001 taaaactttc caaggtgcac cccaggaaat aaaagttcat caagctaatg tcctatcata
    27061 tttctccgga ttattggaaa aagataaacc acaggcactc tatccattgc tcaatcttga
    27121 gtattatcca gattttaaaa attatagcac agaaccctgg ttctggctta tattggcact
    27181 ctgtgcaata tctctaatca tttcaagtct tgtagcaaga gaaaatagat tgaaagaatt
    27241 ggaacctgtt gcttatgctc aaaaaaccgc tcatcgcatc ctgaataagt atttaaaaaa
    27301 ggcaacagaa gcagcagaaa aactctccct tgatttctat cccttggcag aaattggatt
    27361 gatggattat ctggcaagaa aatatggtgt ttctaaaagt ctgcccacac cagaactaat
    27421 tgatgcttta agaacaaaag gtatgcctct tgaattactt gaacagttag agcaattttt
    27481 gttgctatgc cagaaagctc gttttatgcc cggaggagca gaagcagaaa aattggatga
    27541 ggctctgcac caattgcgtt ccctggttca agccttaagc cgttttaaac ctgaaaaaga
    27601 atcctctaaa aagaacagag ttacactgcc tcaaaaggat atcaactcta aaaatgagga
    27661 acaataatga agcactggat atttatcttc ggcttactca tcttaacttt ttccttatct
    27721 gctcaagtct tgaatcctga ttctttaaat gcagaacaag cattctatca acaaactctg
    27781 caaaaattgg aagctaaagc tgcacacgga gttaaaaatg cagatttatt ctatgatatg
    27841 ggggtttgcc attatcaatt gggaaacact ggtaaagcag tgctatactt ccttcgggct
    27901 ctgaatctga attctgcaca taaacaagct aaacaaaatc tcttgtatat tcataatctg
    27961 catccagaac tttctcctga gcctaacaga ccttttctcg tgcagttatt tctaaatatt
    28021 tatgatttct tttctttaaa ccgattggct ataactctct taatattagc tatactttta
    28081 accttgagtt tacattggct aatgcacact ccttcagaaa aggaacgcgg tttacctatt
    28141 ctggctgtgg taataacagc tattctcttt atcgctttcg gtagtatgct gctggtcaaa
    28201 aatcaccgct atcgtaatga ttctcgtgca gtcgtaatcg ctaatgtagc acccatttat
    28261 catactccag aaagcaacaa tgttctccag actatacctg aaggaagtgt ggttgtggtt
    28321 atcaaaaccc agggcaagaa aactcaaatt atcctacctg atggaactca aggttgggtg
    28381 gatagtaatt ctatagaaag agttgtgcct gtaagtaaca ctaaggaata aactatgcag
    28441 aaaccaaaac tatttatcac tcgctatatc cccgaaccag ctattaaaag attggaagaa
    28501 gtatttgaag tggaaatttt cccggaaaac cgagctatca ctcacgaaga actgaaagaa
    28561 aaagtgaaag atattgatgc tttaatctgt cttttaactg ataatataga ccacgaaatt
    28621 atagaagctg ctcctaacct taagtgtata tccgtctatg cagtaggtta taacaatata
    28681 gatgtggaaa ccgctactaa acatagcatt gttatatgta atacccctgg agtgctaact
    28741 gaaactactg ccgacttagc ctgggctttg attatgagtt gcgccagaag aattgtggaa
    28801 gcagatcgtt tcgtacggga aggaaaattt caaggttggg aacctatgct gatgctgggt
    28861 aatgatatct tcggcaaaac cttaggtatt attggtatgg gcagaatcgg aagagcagta
    28921 gctcaccgtg cactcggttt tgatatgaaa gttatatatt atgaccccca agttgatcct
    28981 gagtctcttc cttcagatta tgtttctgtg gatttgacta ctctctgtca aaattctgat
    29041 tttatcagta ttcacactcc tttaactccc gaaaccagac atctaattgg agaaaaagag
    29101 tttaatttaa tgaaacccac tgctattctg attaataccg caagaggtcc tataattgat
    29161 gaacaggctt taatatcagc gttaaaggaa aataaaatcg ctggtgcagg attggatgtg
    29221 tatgaaaatg aacccgttat ccctgaagaa ttaataaagc tttctaatgt tatattattg
    29281 ccccatattg gttctgctag ttatgaaacc aggacaaaaa tggcattgct tgctgcagaa
    29341 aatgccattg ccgtaattga agggaagaat ccacctgctc gtgttaattg aactctggac
    29401 aagatagctt tcaaatgcgc caaaggtttc atttgccggc ttgttaatga gctttatatt
    29461 gcctattttt ctgctctatt aaccaggata aagaaccagt agtgagtgat tataaataat
    29521 ccctgaatga aagaaattaa ctgctctatt taataaccta tggatagact aattcttttg
    29581 ttccgtttta attatggctc tgcaaaacaa agcgagagtt catctatctt cttataacaa
    29641 aaagttattg acggatataa cgattttaaa gaaaaagaaa aaactattaa aatataaagg
    29701 tatgtcaaga tgctcgtggc aaagatattt gtctgtctaa aacctaatgt gcttgaccct
    29761 cagggaaaag cagtttgtaa ttctcttcag caattgggct ataccaatgt gatagaagcg
    29821 cgagttagta aatatataga attgactttt gattctgata atgaggaatc tatctggaaa
    29881 gaaacggata aaatctgcca ggagctactg gcaaatccca atacagaaca ttattggttt
    29941 accctggaaa ataaagagga aagtcaaagt tgagagtaag tgttattacc tttcctggtt
    30001 ctaattgcga ccatgatgct caagaagctt ttgcctatta tggacataag agtcgtttaa
    30061 tctggcataa agatagagac ctgcaaaatc cagatttagt agttttacca ggtggttttt
    30121 cctatggaga ttaccttcgc tgtggtgctt tagctcgttt ttcacctata gtggaagaag
    30181 ttatggcttt tgctaaaaag ggagggttaa tccttggtat ctgtaatggc tttcaaatcc
    30241 tgactgagtg tggacttctt cctggaactc ttttacgtaa tataggatta gattttattt
    30301 gtcagcatca atatattaga gtggaagata ataacactgc cttcactcat cagattccag
    30361 aaggaacagt tatggacatt cccattgccc ataaagaagg caattatttc tgttctgacg
    30421 aaattttaaa agaactacaa gataaccatc aaattgtctt tcgctattgc gataaacagg
    30481 gcaatataaa tgccgaatat aatcctaatg gatcgcggga aaatattgca ggcatatgta
    30541 atagaactcg taatattctt gggatgatgc ctcatccaga aagagctgca actccttatg
    30601 taaccagtaa tgatggagga aaaatctttg ctgcattgga aaaatacttt aatctcaatt
    30661 gataaacttt tctttccacc tgtctgtctt gcttgtaatc agaggataga aagtgttaaa
    30721 gaggttttat gctccgaatg caaagaatta ctttctccta ttacgattaa ttactgtgat
    30781 aaatgtggtg cgcctatgat tgattatagc tgtccttatt gttcagaaaa tgattttgtc
    30841 tttgatattg ctcgtgctgc ttttatttat cagtcgcctg ttcgggagtt aattcataat
    30901 tttaaataca atgcttttct tgctcctgcc agtttcttta gtcaagcaat aatggaactt
    30961 cctgaggctg ataaatttgc taataatttt gatttgatta cggcagttcc tttgcataga
    31021 gttcgcaaaa gagaaagagg atataatcaa tccgaactta ttgccaaaaa gttagctatt
    31081 aaatggaata taccctatgc tgaacttgtt aaaagatatc ttcctactcc ctctcagaca
    31141 actttaaatg ctgaagaaag agtcaataat ttaaaaaatg cctttcgctt agagaaaaat
    31201 gctgatgtaa aaggaaagaa aatcattctg gtagatgatg tttttactac tggaaccaca
    31261 gttaatgaag tatcccgagt tttaaaagaa gctggagcaa gcaaagttgc agttttaact
    31321 gctgctcgtg caatttgatt atgaataaag aatataaaga aattaaaact actatcactg
    31381 aagaagaagc caatgaaatg attgaaaaag ttgctcactt cttcgttgac agaagtttag
    31441 gctctgctgg tattatgctt tttgaatccc ttcatccttt gcatgggatt gcaagtcagg
    31501 ctctatattt tattcttcct tttgcagaaa tgatttttga ctctaatcaa tatcagcgtt
    31561 ttgctcttat gatacaaagt gatgattact ttaaacgctt gattaaaagg atagatgaac
    31621 tggacgaaga aaccaatgaa gaaagaagaa ataaagcaag acttaaaaga cagcgtagaa
    31681 agaatcaacg aaaagccttt tttaaaaaaa tctttaataa aacaaataag tcaactgaat
    31741 caacggaggt atgatgcaat acgatgagaa acgccttacc cgaattgttg ctactgattg
    31801 tggcagcacc actactaagg caattttaat tgaatggatt gatggagaat accgccagac
    31861 cgttcgggga gaagcaccca caactgtgga agcccctttg aatgatgtta ccaaaggtgt
    31921 gattaatgcc acccaggaac tggaagaact tgctcgcctt aaataccata atcctaatat
    31981 caagtttatg gaaaatgggg aatttgtcat ccccaggcgt ggtgatgttg gagtagatgc
    32041 ttatgtttct acttcttctg ctggtggcgg tttgcaaatg atggttacag gtgtggtcgc
    32101 ttcaatgaca ggagaaagtg cagagcgagc tgctttgggt gctggtgcta tagttatgga
    32161 cctcatagcc agtaatgata agagaatgaa tcacgaaaag atagaaagaa ttagagaatt
    32221 gagaccagat atgatattga tggctggagg tgaagatggg ggcacaatta agcatgtagt
    32281 agaaatggct gaattagtta gtgctgctga ccctaaacct cgtctgggat caggatttaa
    32341 actccctgtg atttatgctg gaaataaaga tgctcgtgat tatgtgcggg aatgtttaca
    32401 agataaagtg gatttgatta tcacggacaa tattcgtccc aaactggaaa tagaaaacct
    32461 caaacccgca agagataaaa tccatgaact gtttttagac catgtgatga aacaagctcc
    32521 aggctatgat aaattgatga aatggtgtca gggaccagac catgaacctg ttcctattat
    32581 gcccactcct gcagctgtag gtaatattat gcaagctatt gcccgtgaag aaaatattga
    32641 agttgtagga gtagatattg gtggagcaac cacagatatc ttttccgttt ttaccgaaga
    32701 attcatcttc aatcgtaccg ttagtgccaa tctgggaatg agctatagca tctccaatgt
    32761 tctggcttcc agtggcttag agaatattat gcgctgggta cccttagata ttgatgaagg
    32821 gtatctacgt aatatgatta aaaacaaaat gattcgtcct actactatcc cttatctttt
    32881 agaggaactg gttttggaac aagctatagc tattgaagct ttacgtcttg cttttgaaca
    32941 acataaagag tttgccagca ctttaaaagg aactcaacgg cagcgcgata tatctgaagc
    33001 cttcagtcaa tccacttctg gtgagacgct ggtcaatttg atgactttag atttgttagt
    33061 aggaagtggt ggagtacttt ctcatgcacc tcgtcgtagt cagactatga tgttattaat
    33121 taatgctttt cttcctgaag gtatcactcg tttagctgtg gatagtatat ttatgatgcc
    33181 tcatctggga gtgctttctg aaataagcac taaagcagca actgaagtct tccataagga
    33241 ttgtatgatt tatttaggaa cttgcgtagc accggttggt aaagggaaag tagggaaacc
    33301 tgctcttacc gcaaaattgg aattaccgga tggaagcact tgggaagaac agattccttt
    33361 tggagaagtt cgcttaattc cttgtggtgt gggagaagta gcaaaagcaa acttgaaaac
    33421 ccatggtggt ctaattctgg atgaaaacaa aaataaagaa ttagaagtgg aactgaaagg
    33481 tggagtggta ggtattgtcc ttgataccag aggtagacaa ccttttgtgc ttcccacaga
    33541 aaaagctgaa cgcattgcca ctttaaaaaa atggataaag gcatttaatg cttatcccga
    33601 acagatatta aaataggagg aagaaaatgg cacaagcata ttctgctggt ttaatggtaa
    33661 ctgaaaatat agtcctgaaa aaagaaagag ttctaccact taaaggtcag gtcctggtta
    33721 aaaaaggtga cttcgtccaa gctgaaactg ttgtcgctga aaccctcttg cctggtaaag
    33781 tggttccttt taatttagct aataaacttg gtgtagctcc agcacagctt cccaatttta
    33841 taaaagtaaa accaggtgat aaaatcacta ctaatacagt tttagcggaa acaaaaggct
    33901 tatttggtct gggtataatg aaaaatgaag tgcgctctcc tattaatggt gaagtggaaa
    33961 gtatctctac cattacaggt caaatcctat tacgtgaacc caagataccc gttcaagtga
    34021 aagcttttat tgatggaatg gttacagaaa tcatacctga agaaggtgtg ataattcaaa
    34081 ataaaagtgc ctatgcacag ggaatttttg gcgtaggagg cgaaaccgta ggtgaactta
    34141 tggtcattgc agaaagtcct gaatctgaaa taactccagc gcaaattact gatgcctgca
    34201 aagataaaat aatcgtgaca ggacaattcg tttcttatga tgttattgat gctgccaaaa
    34261 aacatggcgt taaagctatt ataactggtg gcattgatga ccaagaccta aaaaaacttc
    34321 tcggatacga tctgggagtg gctattacag gacacgaaaa cattggcatc actatcgtat
    34381 gcaccgaagg ttttggtaaa attgctatgg caaaaaagac ctttagttta ttgaagaaat
    34441 ttaatggaca taaaacatct attcatggca gaactcaaat cagagctgga gtcattcgtc
    34501 ccgaaataat cattcctctt caattttctg aagaagaatt agttcctcaa gagccaaaac
    34561 ctcctgttct ggaaattggg actattataa gaatcattcg tcaacctaat tttggcaaaa
    34621 ttgctaaagt ggtggcatta cctgaagaac ttactaaagt ggaatcagaa actctggtca
    34681 gagtcttgga agctgagttt gaagatggca caagaatgat tatccctcgt gctaatgttg
    34741 aagtcataga agcataatat tgaccctcta tcaaaggaaa gtttattaga ttagcctaat
    34801 aaaaaattgg gttcaagatg tcttcatagc gcaaagcgga tagccttgtt tacttttttc
    34861 tataggagtt aaaagtcata agaagcatca taaacaagtt ctttcaatgc ttaaaattag
    34921 ccaaaataag aatgcttcgt tggagcttca tagacgagga cagctaaaga aaaaactcta
    34981 tgagagtttg taacaaggag gatcttgttc cacctataag gaaacgatac ttatatctaa
    35041 gcaatgttta agagcttttt taagtctata tgtgtgacta atattttata gataaaacag
    35101 taaacaaatc actttataaa tttagcgata agaatttata ttgttatatg actttattat
    35161 tgttactcaa tcagttgcaa ataacttagg acaatttatc ttctctcact ttttatatct
    35221 gattttctct ttcttctatc ctacctctcc cttagctctt gagcttataa agtgttaaga
    35281 gagaggcaat taggagttta gggacagcta atagaggtaa ctcactataa ataaagagaa
    35341 tatagagttg acgctccaaa ttttatcgtt aaaaaatagt tataaataaa tataaataat
    35401 gataaggatt aggctgaaat aggaattgaa cctgagagat taattagatt taataatacg
    35461 agcatagagt tctccgccac tggcagaacc aaaataaccc agggcattat gaacaccacc
    35521 atgtagataa ccatccacca tataatgaaa tctatagtaa ttatcatcaa ttacccacac
    35581 tcttacgcga tactttccat agaagataaa agcagtagag taatttttta aaaccacata
    35641 ataacctgga agaccttcca acggagcaga ttggaaacga ttagccagtt ctaagcgcct
    35701 gataccaaga cttccttgat tataaagctc ttcaatattg gcggagggat gcatttcttg
    35761 taaaagctga ttagtatatt ccagattggt actaaattcc tccatacaat aggtttcaat
    35821 ccagagatta aatattccca gtgtatttcc ggtgcttacc accagaggat aattggtatc
    35881 aatttgatta aagggaatga tattagtggt ttcgggatca aatgaataac ctgtaccagg
    35941 aggatttatt ccataaggat caggttttaa ttccactctt tgagggacag tggtttcagc
    36001 ccagattaaa gaatccattc ccggaatttg cacttccagc cgataatgat gttcaggttg
    36061 aataatatgt tctgccaaat ctatatactt tatcttaaat tcgtgtaaag aaggagttaa
    36121 agaaaaagtt tctcccgtat ccaaatcttt tataaggaca tctgcatccc agacaaaaag
    36181 ttccagtggg tcaaattctt caatggagct acttctggtt atatagatag gataggaaag
    36241 agagattggt ttatctgcca ttaataatcc tgctacagtg aagacttccc ccttaaatct
    36301 tggtccagat gtattatcac aggaaacaat aaagagacaa ctcagtagta taactgagag
    36361 catcactatc tttatcactt gctttaccat cttacattaa ctccaataaa gggcaaaaag
    36421 ggaaattggg ttccatctct tgtgaccaac ttcaaagtgc catcttcatt catctgcaca
    36481 tcatagaaac gaaaacttac atttttatgg ttgaacacat tgatgatttc cagataaggt
    36541 tcaatagaac cccagaaagt gttccattgt aaatgcacac tcaaatctaa ccttaaatac
    36601 tcgggaagcc gaactctatc ctgataactg tacataagct ggaaattttc accatcaaaa
    36661 aagatgcgtt ctggaatttc agaaggttgt cctgaattgt aggaaaaatt catccctact
    36721 atcatatcag cgttacctat ttgccaacct gtgttttgac tgatattata gttttctact
    36781 aaagaaacgg tgtgagttct atcatagcga ggataaaaat attgtggttc acctgtataa
    36841 gggtctatat taacattatc aatcttacgt ttagtctcac tataagaata acctaaataa
    36901 ccttttaagc ctttccagtc atttcgcagc atcaattcta caccgtaaga atagcccttg
    36961 cctgtattaa ctgcatctga aagggtgcct gtttcactat tccaattgaa aaaagtattg
    37021 gtattaaact ccttgagatg attataggtc ttataataga gttccgcatc ccactggaaa
    37081 tatctgctta acgctctatt ataccctaaa atataatgat gagaaactcc gggtttaaca
    37141 cttccatcta tcggtaacca aacatcataa ggagaattga tgtacaaacc gagaagcgat
    37201 aaatattgat gatacacgcc aaaattaaga tatatacttt cacctacatc cagattacgc
    37261 ttaagagaaa ttcttggttc aagattaaaa taggaagcag aaggaacagg agctggttct
    37321 tccttcaagg ttttatacca gtttactctc aaacctggct gcaaggtcca caattcattt
    37381 attacccaag tatcttgagt aaaagcggaa ttggttaatg aagttatagc tgcttgcggt
    37441 aatgcatttt gggcaatttg ataagtagtc cccatcttta aatcggtatc attaaatttt
    37501 agctctattc cggtctcaat atcgtgatga ttactgggtt tccagttcag agttactttt
    37561 accgttaaat cacgaatata attcttactc tggaagatgt tcattccttc cgctgagact
    37621 tcatcaatag aattattata ctgacttccc gaaatgataa attgtgagaa aagctgtggt
    37681 gaaaatagat gcatccattg agctgataaa atgctattcc cccaatctaa gtccataata
    37741 ggagtaatat cataggacaa cacatcttta ccgtagtagc cactgaaact tagcttattg
    37801 tttgtatcaa tatcccagtt aattttaata tgagcatcgt aaaaatagta atcaggcaag
    37861 tcagagataa tcatttttaa catttccaga taggtacgac gaaatgatgc cataaaagaa
    37921 ccactttgtg aatctatttt ccagggtcct tcagcagtag ctgaagcaga aatgagacta
    37981 agtctcccca ctccttcata aaactttctg ttaccatctc tttcagtcac atccagaact
    38041 gaggacatcc ttcccccata ctttgctgga taacctcctt taatcaattc aatactttca
    38101 actgcatcgg tgttaaaagt gctaaatatt cctccaaagt gattagggtt atatacctca
    38161 gtattatcta aaagaataag attctgatct ggagaaccgc cacgaacata aagtccagca
    38221 aaaaaatctg agatagggac cactcctgga acagccaaaa ctgctctaaa aacatctgct
    38281 tccataggag ctacaaccat ctggatatcc tgtcgcgttt gatgaagagt gctgggttga
    38341 ataataggtc catcactttc atcctttgtt ccctttacta taactggact caattcaacc
    38401 gaactcttaa ctaactgcat atttattacg aggtgatcat tcagactttt tatagtgatg
    38461 ggcaccgttt cttttttata tgaaactaaa gagaaagtaa ggataaaatc tccggtctgc
    38521 tgtaaattta agacataata tcctttttta ttggtctgac atcctatctt cgtatcggca
    38581 atatacacat taacatattg aatgggttca ccactatcgg ctctgctcac aaatccacta
    38641 attgtggcac aatgcaatat accacaaaga aaaataatta gcaccattaa gaggattttt
    38701 ttctgcattt cttcccattt attcattcat taaatttact tatttctggc ttgataaaga
    38761 tgtcaataat cttattatga aatgggcagt ctataaaaca gaaaaggagt gacacacaag
    38821 tcactccttt tctttcagag gttagataat cttatgagta ttatttggca agtaacatct
    38881 taccagtaag ttttttgcca ttattttcta aacgatagag atagatgcca ctggaaacag
    38941 ttctacctct gtcatcagtt ccattccaga caacattgta tttaccagct tcacgaacat
    39001 cgttaaccag ggtgcgcact acttcacctt tcaggttata gattttaaga gtagtataac
    39061 ctgaggttgg cacactaaaa ctgatagtag tttccggatt gaaggggttc ggataattgg
    39121 ttgcagacag tgtgtataca ggaatcacat tgtcatcatt aggtgtatca taccacacga
    39181 tagccctaat cattagttcc ccttctgtga ttggttccca atttgtagtt gtccccattt
    39241 tcttaaaact attaccagaa gtggatgtat ccagtcctat agctgaacca ttagcaacat
    39301 ccaggatgcc aatgtagaat tgaccatctt gtacatctat atcgggtggt aactcaatat
    39361 aattccaccc ttggactaat gaagctacag gatatgaata ttgagcaagc tgagatccag
    39421 gcataccatc aggtccgtca ttatcaaaaa cacggacaat tagaccagca ttgccgacat
    39481 tctggatata aatcttagcg tatttgaccg tcaagctacg attaaagcta aattttactg
    39541 ccatttgttt attagcacca acattgaagc cctgatctgc tgtgccatca tcatgggata
    39601 attctaccat agattcaggc ataacaaaca cggtaactac atttgatgct ccggattcat
    39661 tggtgccata catagaagtt acataatagc tatgaattcc accaggaaca ttgctatcag
    39721 tgtaggtaag tacatcagca cctacctgat caatttggac attatcacga taaattctgt
    39781 agccatctac attacgtttt gctttaatgc ctttggcttt aaattgagca ttagatacag
    39841 gttgaacttc ctgtttaagg ggggcataag aaagctcata ttcctcgcct ttggcatttg
    39901 cgacataaat cctaatgttc cagttataag taaggctgga atttaaattg gttaaagtag
    39961 tccagctatt ttgccaatac atcatattac catatccctc cacttgagga cctgcatcac
    40021 aacccgcagg atatcccgtt tgagtattgc agcggtatcc taccataatc acagtttgag
    40081 caggaatagt gaaaggagtg gtcaatataa cttcattcca agcattaata gtgggtgtag
    40141 gaacagcttg ttcataaaca agagctcccg tagcaccagt ccaaatccta atggtatagg
    40201 tgcagttagc ttcatatggg aagaaacgta ctttagtgat attcattccc acatagtcat
    40261 agatactatg ctcaccaatg ggatcccatt ttgctgctac ggcgaaatct gctgcggaac
    40321 ctgttccaat agcatcagca ttctctccgt cataatgtag ccagccttct tgtcctccac
    40381 caccaccagt gccaggagct gcccagctaa gcatcacatc gcgaccgcta actacagctt
    40441 ccaggttttc cggaggagca ataaacactt cattataaat ggttacatca tcaatcataa
    40501 tacctgttcc aataggatta tcagcatcgg atttaaataa ccatcttaat ctgatggtat
    40561 gacccgcata gtcactaata taaccatcaa caccagcata ggatgcaacc attgatgccc
    40621 aggtaggtgg agcatcagaa taaacataat tgcttaaatt gggatctcca taagggttac
    40681 tcatataata ccaagttaaa ccgttattag gagagatttc ccagccccaa tagtcacagt
    40741 taggaaatgc atcaggatca ctaaagttac cttgaatcat aaaatctact ctaatatcac
    40801 cttcattagg gagctgaatt ggagggctca ctaaataatt catcatatta gggttataag
    40861 ttccattctc attctgacat ctcattacat gaggtggaga tggtgcagta ggatcggttg
    40921 ctaatcccca gaggtctccg ccaacagcaa ctaaacttgc ccaggtcatc tgaccatcat
    40981 caacaccatt attggtatat ccgccaaagg aaatatcatc taccataacc ccaaataaag
    41041 tgggatcatc agtagtgcaa taagcaggat cagaagcaaa ggcaaaacga atctttacac
    41101 tctgtcccac atagctggaa aggttaaaag atacattagt ccacgcagga tgaattccac
    41161 cccaaccagg aatgtttgga ccttcattat gctcaaaacc gaaagcataa gatgaagtga
    41221 aatcataagg tggactggta ggagtaagaa ctgtccaggt tgctccatta ttcgtggata
    41281 tacgaacatt gaaggaatcc cacccattgt atactccggt gccacctact tcttcaagag
    41341 caactgccat tttgaaagta agcactgaat tagcagctga tatagtacga gcaggtgtat
    41401 ccagaaccag atactgatgg ttataatatc caccaatatt tgttccctgt gcaagagcgg
    41461 gatctcccat ccaccatacg gttccctggg ttccaccata attataaatg tgccattggt
    41521 ttgggggaac actagcatca aagtgggtcc acccttcggc accgttctca aatgtttctt
    41581 gccaggcaat tatctcataa cgatttgaac cgtttgcgaa cattgccgtg acaagaatca
    41641 aggctaagat aatcagccat ccttttttca ttgatcctcc tctttttttg aagaatatta
    41701 gtataatctc ttatttaggc tcttttaggt ttttgtcaat gattattttt ctttattatt
    41761 gcttattata agtatatctt tctttttcaa gccttttcta tcttacatct ctttatcacc
    41821 ttagggcatt ttacacacta tgaactatct aactggaaca caattttcaa aaaacagctt
    41881 tattttctaa ctctttattc ctgtctttat ttataacata attgatagac cttacttgtc
    41941 aaatcccttt tttattattt ttattgacgg gtaggaaaac ttaaaagaaa agaaatatta
    42001 ttaaattata tgataaataa ggagatagag atgtttctaa ctgatcacca attgaaaagt
    42061 gaaattctgc gttgcgaata ctgtgctgaa aaaccctgta aaacagcctg tccttgtgat
    42121 tgttctccag ctgattttat gatggcagct gctgaaggta aaccttcgga ttataaaaga
    42181 gcagctggaa ttattttatc cagcaatccc ttaggtggaa tctgcggaaa agtatgtcct
    42241 gatgatttct gtgtgcaagc atgttctcat tataaatttg atttccccat aagaattcag
    42301 gaggttcaag ctactattat aaataaagct catctgttag gtcaattacc agaatttcag
    42361 caagtcccta aaaatgggaa aaagattgcc atagtaggag gaggtccttc tggacttggt
    42421 gctgcagtga ccttaactca attaggttat gaagttgata tatttgacgc tgaaccctta
    42481 ggaggtcaaa ttagactcat ccctccagaa agacttgata ctgaagttat aaataatgat
    42541 ttaaactatc ttaatcgcgc attcaatttc actcatatcc aggaaaaagt agataatgtt
    42601 caggagcttt tctcaaaagg atataatgcg gttctgcttg cttgcggttt gaatgaacct
    42661 ataaaactca atattcctgg ttctgaaact gaagttttgg gtctggaact tttgcgtaat
    42721 ccagaagctt acgaacttaa aggtaaaagg attgctctgg ttggaggtgc tattgctgct
    42781 gatgcggcac taatatcatt aagaaaaggt gctaactatg cggaaatgat aactttagaa
    42841 gcctacaatg agatgcctat aactgaagat gaaaagcaaa aactgattga agctggagtt
    42901 gcctttagtc cgagaactcg tatcactgct ataataaatg aaaatgggaa aactgtggga
    42961 ataaagacta aaagagtgac acttccttct gggcttacct ttcatccttc caatatcgta
    43021 gatgaagaag gctccactga aatgtataga ccttttgata tggtcgtaat agctatcggt
    43081 aatcgttgtt ctttaaatga acaaggtact catatttatc aatgtggaga tatggtcaat
    43141 ggtccttcca ctgttgtaga agcggttgct tcaggaaaga atattgcctt aaaaattgat
    43201 gcagacttaa gaaatcaaga ttatactgag ccagaaaagc agactaaaag tattatttct
    43261 attcctcact ggaagcaata tcccgtttct ctggagacag atttctttgg tagaaaaata
    43321 aaatctcctt ttctactttc tgctgctcca cctacagatg gttatgaaca aatgaaagca
    43381 gcctatcttg caggctggca aggtggcata atgaaaactg cttttgagaa acaatatatc
    43441 catattcctg gagaatatat gtgggtattt aatggaaaga ctttcggtaa ttgtgataat
    43501 gtttcagagc atcctttaga ccgtgtgtgt gaagaaattc agcgcttgat taaagaattt
    43561 cctgatcgtt taactatggc ttcaacagga ggtccagtaa ctggaaatga tgaatccgat
    43621 aaagctggct ggcaatccaa tacccttaaa ttagaaaatg caggtgccat gggcatagaa
    43681 tatagtctct cctgtcctca aggtggagat ggaactcacg gtgatattgt ggctcaaaat
    43741 gctgaattga ctgccaagat tatagattgg gtaatggaga ttagcaatcc agagattcct
    43801 aaacttttta aattaaccag tgctgtaaca gctatcgttc ccatcattaa agcggttaaa
    43861 tctgttttta ataaatatcc agagaaaaaa gcaggaatca ctctggcaaa taccttccct
    43921 gctatgggtt ttagagaaaa agaggctgca ctctgggatg aaggagtgat aataggacta
    43981 agtggaaaag gtatccttcc aataaccttc ttaagtttag caagagcttg caatcttggt
    44041 gtgatagttt ccggtaacgg tggagctatg agttatctgg atgctgctaa ttttcttgct
    44101 ttaggcactt caaccgttca attctgcagc atcgttgaaa aatatggcta tggaatcata
    44161 gatgagcttc attctggctt aagttatctt ttggaagcaa agaaacttaa gtctgttcaa
    44221 gaactcattg gtatcgctca acctaatcct atcaccgatt ttctacaatt gcctccaaaa
    44281 tctaagagta gcactttaaa tcaaaatcta tgccttcagt gtggaaattg cactcgttgt
    44341 ccctatcaag ctataacttt aaatgaagat aaatatccgg agattgataa agataaatgc
    44401 atcggatgtt cactttgcac taagttgtgt tttactggtg cattatatat gagtaaggat
    44461 agctaagcta aagctcagct atccttagct ttttaaaagt ttttaataaa tacttcaagt
    44521 tgcctgagaa tcagggaagt ttatctggat attttttatt tcagcatatt tctttaaatg
    44581 ttctctaatc tcttcagcaa tattgtctct ccattggtca aaattcatta aattaaggtc
    44641 aattatcact tccccatcat taaatccaac atacttaacc atcttcaagg ttacaatatc
    44701 ttctcccact ttaggataga taatctttct taactcttca attatcgtat ctttgccaaa
    44761 attaggaact tctttaagtc catgagcaat ttcataattg gaaatagcac tttgtagagt
    44821 atctactgcc aggacagcac aatgaacttt gacaggaggt aagcctccga gttcttccat
    44881 agcttgttgc caggtgattt tttttgcctc atccagtgtt tttccttttg ccagatctgt
    44941 tataattgaa gctgtagcga tattagaagc acagccatag gaaaggaact ttatatcttc
    45001 tataacctta gtttctggat tgactttaag aaacatcgtt acctgatcac cacaggctgg
    45061 acttccttca gttgcttcag catcaggatt ttccagctta cccacattat gaggatgcat
    45121 aaaatggtct aagactatct gtgaatactg catttatctc tccttgttat gtattgcttg
    45181 ataaagagga ctgcgttctc tgagtgttgc tgtaatttcc gctaactttt ctaccgtata
    45241 atctatttcc tctttagtat tgtatctgga taaggtaaat ttcatagaac catgagattg
    45301 aacatgattt ttacccattg ccatcagcac atagtttggc ttcaatcctt gcgaagcaca
    45361 tgctgaacca gttgcaacgg ttataccatt taaatcaagc atcatagtta ttgattcacc
    45421 ttcaatatag gaaatagaga cattgatgtt atgacacact cttttgtctc ctctgggtcc
    45481 attcaattct atatctggaa tggttgattc caatttcccc aacaaataat cagacagctc
    45541 tctaagatag gaaatattag catccaggtc attgaaagtc atttccaccg ctttggcaaa
    45601 accggcaatt agagcaatac ttaagccacc agtctgtaac tggtcaactc tggtgacacc
    45661 atgtattaat tgacttagct tagttccttt tttaacaaag agagctccaa ttccttgagg
    45721 accatgaatc ttatgagcgg aaacacttaa agtgtcaacc cctaaatctt gcacatccag
    45781 atgcattttc ccaaaagctt gtccagcatc agcatgccag aagattttat ggtctgctgc
    45841 ctctattacc tttttataat cttccatagg ttgaattgta cccaccacat gatttactat
    45901 ggtgctcata aatagtatgg tatccggtct gattgcttct tttaacttat ctgcactaac
    45961 ccaaccttca gaatcaggtt ccagatagct aacttcaaaa ccctgttttt caaaataagc
    46021 agcattagtg agtaaatcag ggtaatccac cagagagaca atgatatgtc ttcctttatt
    46081 tgcatttgct cccattaacc cttttagagc aatgttattg gctaaagtgc cacctgaagt
    46141 gaagtgaatc tcttctggta aagaatgcaa agcctttgca agaatctctt tgaattctgc
    46201 cagagctgca ttagtttctt ctcctgtact gataaaagtt ccaggaaacc agaacttttc
    46261 cagaaaatag ggcttcatcg cttccagaac ttctggagct aatggagtgg tgacacaatt
    46321 atccatatat attctatgat tattcattaa tcttcctccc gcatcttttc tgctatcaaa
    46381 gcaagtgaat agcttttaaa aagttcccgc tgtttttctc ctatttcaat aaaaatgggt
    46441 ctgagcggac aatcttctcc ctgacaaaat ttccgtccca tattacaatc caattctaat
    46501 ctatggtcat ccactgcttt cattacatca taaagtgtta tttcatctgg agattgtgcc
    46561 agaacataac cacctttcaa accagcactg ctgataatca atccagcatt cttcaatgag
    46621 ctgagcaagt gctcaatata ttttttaggc aattgctgtt gattacagat attttgagct
    46681 gatatggtgc cttcaggatg ttctgcaatc tccactaatg ctcttagagc atattctgtt
    46741 ttagtactaa ctggcatttt ttctctcttt tttctctttt taaatatata gtaatatact
    46801 atgagattaa aacaagaaaa atctattgct tttttattaa gctccagttg acagaaatcg
    46861 ctccttttca aaactttgat tgagataaac agatgattga gatagaaaaa ctttgttgtg
    46921 cttatgaaga tgagctggtg ctcaaagatg tggatttaat ccttccagaa aatgaattta
    46981 ctgttctggc tggacctaat ggagcaggaa aatccaccct aatttatgct ctacttggtt
    47041 tcttaaaacc aagaaaaggt aaggtgcgca tatttggaag acccctttat tcctataaaa
    47101 gaatagaact tgctcgtctt atagcttttg ttcctcaaga aagtatattt caatttgatt
    47161 atccggtgcg ggacatagtt ttgatgggaa ggtaccctta tttaggttta acgcaaaact
    47221 ggatggcaga agattatgaa gcagtagata aggtcttaca acagctttcc ttacaggatt
    47281 tagctcatcg tttttattct caactgagtg gaggcgaaaa acaaagagtt ctcatcgctc
    47341 gtgctttagc acaggacaca cattatattt ttctggacga gactctttct caattggata
    47401 tcaatcatca aattgatata atggttcttc tcaggaagat tctggctgaa aataaaaagg
    47461 gaatcctgct catttctcat aatctcaatt tgagcgctaa tttcgcagat agaatggtat
    47521 tcctttccag gggaactatt ctgggctctg gtactcctga agatatgatg aaacctgagc
    47581 ttttaaaaga actttttggt attgaacttc tcactatgct aaatccttta agcggaaaac
    47641 ctaatatcct ttatccagga atgaaatgaa atcctggata gttactcttt gtttagctat
    47701 aattacagtt gccttgactg ctgaatctta tcttattcaa gcagttgtag tagatcccaa
    47761 agagcaccct gtttcggaag tagtaatttc agatggaagt aaaactgttt tcagtaatga
    47821 aaagggttat tttttgatta atacctctgc ggacacatta accttccata gattggggtt
    47881 tcaagagaaa aagctttcag taaagaatat aggcaaaaaa gtaatactaa cccctgaacc
    47941 agttgttctt cctcgtataa ctgtttctga tgtagcctgg aacattgttt cacctccacc
    48001 agatagaatt tcattaccta tagacccaga ccgacattat tattcagcag gtgaagtgct
    48061 gaccactaat cctgctattc atagtaatga tgtgccactt cctggggaat cacaaaatat
    48121 ttctatcctg ggaaatcttt cccgtcatag tcttattatt ttagatgggg tacccttaaa
    48181 tccagatgga agcagttttg acctttcttt acttgaccct aataatattg aatcagtaga
    48241 tattataaaa aataatgtct cggtttatgg gggaagctct gctattggtg gcattgttat
    48301 gataagcacg aaaaaaggat tgcagcaaac agggaaacaa ttttccatta gcactgaact
    48361 gggttctttt ggctatgctc aaaattcttt gagttttact tttaatcaac cttctaccat
    48421 attccgaatt aatcttagca atttaagcac cgataatgat tttcgctaca aagttcctga
    48481 gtggtggtct cccgatagta ctgccattcg ttctaataat gctaaacatc aaaattccct
    48541 tgccgcatct tatactcatt taaataaaaa gtcccatttc agctttcaaa ccgaatatct
    48601 ttcttttatg cgtcaattac caggaacggt taatttcact gagatatata aaaatgcttc
    48661 tttatccggt tgggcaaacc gtaatagatt caatctgaat gctccacttt ttagtggaga
    48721 attgggaact ttaatctggt tgaatttaga ccagaccact tatgacaaca ctaaggcacc
    48781 tttaccggta tatctttcgc actataaaca aagtttactc aatgctggaa ttcacggaag
    48841 ttatggacaa aattcttctc ttactccgga attatcttta aatacaagtc tttctgctga
    48901 agctggatac aacagctatc aaaataataa tctcctgaat agtgcaaata atattgattt
    48961 ttcttccaga tttgccaatt ctatcctaaa aacagaactt aaaatggata aaggtgagtt
    49021 catctggtct aattctggag ctattagata tgatttaact gagcaagaaa atgaatttac
    49081 ctggcgtctg gaaagcagtt tgcaaagttt gggttatata gaaacaacta ttggtggaac
    49141 tctgggaact tcttttgcac ttccttcacc ttatgactta tactggaaag gtgattctca
    49201 agctctgggt aatccagatt taaaaagtga acattcacag ggttggcagc tctggatgtc
    49261 caatcaatgg aataaattat atctgaaaag tgccttccat aaaaatgaca ttgaaaatct
    49321 cattcaatgg cgtcaaattc aaatgtgggg caatgtgtgg aagcctttga atataggtaa
    49381 agcacggata aaaaatgtag aaattgaggc aggatggcaa ccctgggaat ggttaaatct
    49441 ttctaccagc gcacttttta ccaatgctaa agatattagc gaacataatg aaggagctgc
    49501 tccctattta gtgtatattc caggcagaaa ttattcttta aaaatggaac ttaactggaa
    49561 aaagttgaaa ctctggagtg attatcatta ctctggaaaa caatatacca cccccgataa
    49621 tctcactgaa ccttttcctg gttattcatt acttgattta ggattgaatt atagtatgtt
    49681 cattcaggga tggaatgtct cacctttctt taccattaga aatgccctta ataaacagta
    49741 tgaaatttat gcttatgttc ctcaaccagg aatagctttt tatggaggag ttagcttgca
    49801 ggtatccaat ccctgataag ttcctcaatc aactgataat tacagctttt attcaaggct
    49861 ttcataaaaa actttttcca gtgccgcaat atgttttttt actatcttat ctttcattct
    49921 ggcagaagga tagataatgc ttttaccttt tttatctatg cctccgactt caatgagaat
    49981 agctctggta ttatgaatgt ggaagaaata gtttcttgca tttcccacgc gagaagaagt
    50041 tgccatacta acttcatatt ttccaggaag ataatccttt tttaaattct ctgcaagctt
    50101 ttgtgcaaac tctaaggtct cttcataaat tcttttctga ttctcgtttt cttcatctaa
    50161 ataaggtaaa aatatctttt cactgtaggc tccagtagat gaactatgat aaaagattgc
    50221 aaagtcaaag tgttgcttct gagcaagaga aattattgct tgaacttcag gttccgaagc
    50281 aggtgtgtca cctttataat atctatgcgt tggtggtaga gttgaatctt catcccaaaa
    50341 aactggataa ttacgattca agtccactcc atcccattgt aattccagtt tattattctg
    50401 attagtatcc cgattatttt tacgtttata tctatataaa ccctcagtaa aaactctata
    50461 accttcagga ttaatagtag gaactatcca gaatttatat tgatgtaaaa gagccttaat
    50521 ttttttatca gactctgctt ctttagccag tttttctgcc caagaaacta caatttcaac
    50581 tcccagaacc tcgtcactat gatgctgtcc tatcattaaa actttttttc ccgcagaaga
    50641 atatcctatt tccaagccat aaatgggaag ttcttcagta ccactaaaac cgagaatatg
    50701 caacttgact atctcaggat ttatctcctg tatcttcagc atcttagaat ataagtctct
    50761 ctccaatatg tatcctttat ctaagggata gatatttttc tgggctcctg ccacagaaat
    50821 aaatagtatc cctataacta ttaaacaaaa gcattttgtg aaatttaatg acataaaacc
    50881 atcttagatt tttcctttct ttcgtcaatc caataactta tcacttaatt taaatttcct
    50941 atatacttcc ctatctaatt atatagcaga tagttagcct tgattctcta taactcattt
    51001 tcaatcttgt cttttataaa atatttcttt ccccctacct gcatccctcc agcttatgtc
    51061 cagctcatgt ccagcttatg tccagcttat gtcccaaatc ggacataagt tagctatgaa
    51121 tccagcataa ctttctcaaa taaaggatag taagatggat aacttaacta aatattctgg
    51181 ctgcagaata ttgtaattga ttatatttta tcaatgtatc tggagattct ttactgttct
    51241 gcttttgtct gattgcatag actcttatct aagtaaaaaa tctttacatt gtgtttgcac
    51301 atatctaaaa ttagactaaa ctatattgta aagaacaata taagttgact gtaagttaga
    51361 aaaattgata gtgtcattag agaataattt gaagcgaaga agaaataaaa aattaaatat
    51421 tttagacctt aaattaataa agagaattca aaaataatta ataatataga tatataagga
    51481 gtaatagaaa tgaagggtgt aagacaaata gcagttgacc agttggtcaa ttatttggca
    51541 accgacccta tacagaaaat gtctaaaatt atgtctttag ccaagaagct ggatagtaag
    51601 catcagtatg aatattatat agatggaatt tataacgcac tgatggatga gaatagcaat
    51661 tggcacaaat ttacagaaaa tttatttaaa gaggtaaaca caaaatctat ccgtaaactg
    51721 gctgaatgtt ttatggtcaa ttccacattg gtaggaggac ctcgtcggga taaaattaaa
    51781 gccaaatata attgcaatgt tccctgggct ttattaatag atcctaccag tgcttgcaac
    51841 ctatcctgta caggttgttg ggctgcagaa tatggaaaga aatataactt atcctatgat
    51901 atcttagatt ccgtggtaaa gcaaggtaag aaacttggaa tcttttggta tttattttct
    51961 ggaggcgaac ccctcattcg caaagatgat ataatcaagc tatgtgcaaa gcatcaggat
    52021 tgcttctttt ttgctttcac caatggcact ctggttgatg agaaactttg tgaagatatg
    52081 agaaaagtgg gtaattttgt tttagctttc tctattgaag gtgatgaaga agctactgat
    52141 atgagaaggg gtaaaggttg ttatcaaaaa gtaatatcag ctatggaact tatgaagaaa
    52201 catcatttgt tatttggtta ttctacttgt tatcaccatt ataatacaga aagtgttgct
    52261 tctgatgaat tcatagatga tatgattgct cgcggatgtc gttttgcttg gaactttact
    52321 tatatcccca ttggaaaaga tgcaaaatta gattttattg ccactccaga acagcgtgct
    52381 tatatgtata aaagaataca ggaaattaga gccaccaaac ctatttttgc acttgatttc
    52441 tggaatgatg gtgaagctgc tcatggttgc attgcaggag gtagaagtta tcttcatatt
    52501 aatgctaatg gagatgttga accttgtgca ttcattcact atgctaatgt gaatataaag
    52561 aatgcaacct tactggaagc gcttcaatcg cctctcttta tggaatacaa aaaacatcaa
    52621 ccgtttagcg aaaatatgct taggccctgt ccgcttctgg ataaccctga aatcctgatg
    52681 gaaatggttc ataattccaa tgcctattcc actcaactgg aaggaccaga aacggtggat
    52741 gaactttgtg ccaaaactat tgctgctgct gaaaaatggg caccagttgc tgataaactg
    52801 tgggaagaac gacaaaaaga attggcttca agaaatcctt ctccttaaaa caataaattg
    52861 acaatcccga aaaaagggga tgaaccttgt tcatcccctt tatttttgcc tataataatc
    52921 aagtgtattg tagtcgatag aggtcataat ataaaccttt tttctccagc aattcaaaat
    52981 ggcttccctc ttcaacaatc actcctttgt gcaacacaat aatgcggtct gaattttgaa
    53041 tagtggaaag cctgtgtgca attactatag aagtacgatt gtgcataatc ttttgcaaag
    53101 catcctgaat taacaattct gtctctgtat caatattgga agtggcttca tccaggataa
    53161 aaatagcagg atcataagcc aacacccgtg caaaagctat aagctgtctt tgaccggtgg
    53221 aaagtgttgc tcctcgttcc attactggct catcataacc caaaggaagt ttactgataa
    53281 acttatcggc attcacatat tttgccactt gaataatctg ttccttactc aattgcttat
    53341 tgttcagggc aatgttatcc ctgatattac cactaaagat gaatacatcc tgttggacaa
    53401 tacctacatt ttttctgata tcttccacag cataactttc cagcggtttc ccatccagta
    53461 aaatatttcc tttctgatag ggatacattc ccagaattaa atttactatg gatgttttcc
    53521 cacttccggt atgtcctacc agagcgattt tttctccagg ctcaatcttg aagctaacat
    53581 ctttcaaaac ccattcatca ggattatacg ccagccacac attcttaaat tcaattgctc
    53641 cttgcagtct aattcccgtc tttagatgtt cacgatagtc ctctgggtct aattccatca
    53701 agtcaaaaat cctttctgct cctgccagag ctccctgcaa tacattaaac ttttcggaaa
    53761 agtcatctat aggttggaaa agtttggaaa tatattgagt gaaagccatc aataatccga
    53821 tagtaatagc attacgaagg atttgacctc caccatacca aatgagaagt gccactgcaa
    53881 tttgagaact gacatggatt ataggtcgga aaaatgcaaa cagtttcatc tgcttcatag
    53941 aagcattgta gtattcttga tttatccggg caaattcttc tctttttctt ttgtactgat
    54001 taaacaattt aattatcttt tgaccactga tatgctcgtt cagggtggca tttactgcgg
    54061 caagatgttt tcggacttct ctatatactg gacgggtcct tttccgaaaa attacaatga
    54121 cccacactac accaggtaag atagcaaaac taaccagagc taatttccag ttcaagagca
    54181 gcatcaagac tactatggca ataatcatta ttatatcctg gataatagtg ataacacctg
    54241 atgatagcat ttcatcaata gcccggatat cgttcgtaac ccgggtgacc aatcttccca
    54301 ctggatttac atcaaagaac ttaaccggca ttttttgtag gtgtgcaaag gcatcatgac
    54361 ggaggtcatt catagctttt tgagaaaata tagtggtaat aatcacttga aaatatcctg
    54421 cgataaacct tatgatgctg ataatcaaaa aagctaatgc cagccagata agtttgttag
    54481 cagcctcatt tctaagctga aaacgctcgc ttttaggaac tttatttaat tgctcgcggg
    54541 agatagcaat ctgtccatct ccaaccctaa accatccttt aaaaccttct ttacctccct
    54601 ttggattctg atcttctgga agatatttat ttagaatctg aatattctct ggtttgtcaa
    54661 cgataagaaa tactttttcc tcgcctatga ttcctttatt tttaaattcc agtatatctt
    54721 gctgattaat tttattcaaa tcggcagaat tcaatattac aaagtatctg ccctcatagg
    54781 ttaatctgtt aaggttcaga taggaatact tatttagaaa agaattagct gtggtttcat
    54841 cttggaatac agcaatattt ttatcggata ctatataatc atctatagct cttcgttgaa
    54901 ttaaaggttg caccaattct gccaaggaaa caaccataac tatgagaaaa gaaccgatta
    54961 ccccagcaat ataaggcttt aaataatgca ataaacgacc atataaacgc ctatcaaaga
    55021 ttctgttttt tatttcatct tgttcaatta caggtcttcc aatgccatta tcgccaccac
    55081 ccttcatagc tcttcccctt ccagtctggc tctaatgcgt tgcttctcat aaaggtcgct
    55141 atatagacca cctttggcaa tcagttcttg atgagttcca tgctctataa tttgagcatc
    55201 acttaacaca atgatattgt ctgcatgctg aatagaggaa atacggtgtg ctattatgat
    55261 atttgttttc cccttcctat cttcaaccag attttccagg atatagcgtt cggtcttggt
    55321 atccacagca gaaagtgcgt catccagaat caatacttcc ggattggtta atagagctct
    55381 ggcaatagcc actctttgtt tttgtcctcc tgaaagggtc acacctcttt cccctatgac
    55441 agtttcaaat ttatcctcaa attccataat ttcatcataa atttcagctt tctttgctgc
    55501 ctcatatact gcttcaattg gtgcatctgg attacccaaa cgaatgttat tggctatggt
    55561 atctgaaaaa agaaagatat cctgaggaac taatacgata tcccttctca agacttgtag
    55621 tggtattctg tgaaccaaat gaccatcaat taaaatgctc cctcttggag gttcataaat
    55681 tctcactata agttcaatca gagtagtttt accacaaccc gtaggtccta caacagctaa
    55741 ggttttcccc tgttctactg aagtgctaat atggtcaaaa attaaaggaa ggtttttagc
    55801 ataacgaaaa gtgagatccc ttatttcaat attaccctgt aaagctttaa tggaaaaatc
    55861 cgcttcttta tcatcaatct cggattccgt ttcaaatatt tcatttagtc ttttaagaga
    55921 agcagttcct ctttgataca tatctactat ccagccaata gctaccatag gccaaacgag
    55981 cattcctaaa tactggaaga aagctatgaa ttggccaata gttatgtccc ctcggatagt
    56041 ggctctgcct ccaaaataaa gagttataat catagaaaga ctgataataa aaccctgaaa
    56101 agggtggaaa aaaccaacta tttttgccat attgatgttt tctttaacaa aatcccacga
    56161 taccttatcc actttttcca attcagattt ttcttgtaca aatgctttta caatgcgaat
    56221 tccggatata ctttcctgta ttgttccagt caatgtggca aaactggttt gaacccgata
    56281 gaaactcttg tgcatttttt tgccaaagat actaatggtt atagttaaga taggcatagg
    56341 aataacagcc agagcggtta aacggaggtc tatggaaccc ataaaactga aagaagcaat
    56401 agtcatcaaa actatgtcca tagcagctat cagacccata ccaaacatca ttctcacgga
    56461 attgaggtcg ttggtggcat acgccataag gtctccaact ttccgtttat taaagaagtt
    56521 ttgagacagt tgcattagat ggtcataaaa atcctgccga agattctttt ctatcataaa
    56581 agaattgcca ataattagca ctctccagaa atatcgtaaa accattactg ctatggctaa
    56641 gccaataatg actaatgcca gtaataatag tcctctggaa tctatggtcc gctcctgaat
    56701 cctgtctata gcttgctgca taacccatgg tgttatcagc tgcactatat ccaccaagat
    56761 cagcatcact aaaccgccag caattcttcc ataactcttc ttcaagtatg gaataacggc
    56821 atcaaaagtc cgcattcatt ctccattttt tatgttctat ccgaagaaat aacatatata
    56881 ttaaagggaa aataatattt tatagttttg taaactatgc agggaaggtt gataatacct
    56941 ttatcatttg tgattataca accttcaata tagttcaaat taatggtatt aagaaatttg
    57001 tcaaattaaa tatggaactc catatcacaa caatgagtta taaaattcaa ctcttatcca
    57061 actctaaaag ctctctactt attccagtct ataactttta gtctatctat aatccttccg
    57121 aacaatcaag atttatctat tttaatagca tcatccgctg agtgagtacc ttgttttttg
    57181 tctccagacg ataaaaatag actccagacg aaacattctg attcctgcta tccttaccat
    57241 tccacactac attgtaactt cctggtgtaa aattatcctc aaccagaact ttgactaatt
    57301 gaccttttat attataaata gacaacttag ccttacctgc ttctgccaga tcaaaagata
    57361 tagtggttgt gggattaaat ggattgggat aatttgtttt tagtcttgtg attaaaccag
    57421 gtgtggaggg ttcgttcaca ttttgataag gaaatataaa ctcaatagta tcagaaggac
    57481 atccttccat attgtaatat aaagctcgga tataatattt atataatcca ttaagcgaaa
    57541 gataatccgt atatgaagtg atattagtgg tttggactaa ttcaaaagga cctgtgttaa
    57601 atttcttata tactttatat ccaccaatag gcaagactgg atcataagga ggttcccaac
    57661 tgattaataa ttcaccggtc tcattattaa ccacaaaatc tgaatttaca ggcttaggca
    57721 aatctatgag agtgaaatca gtttgcctga gaggagattc aggtgttatt tgaatattaa
    57781 atgcgctgga agattgatgg taaagtaagg tggcagttac attataagtt ccgaaaggta
    57841 aatataatct ataagaataa ttatcatctg gatgggtggc aaaatgatta ttagccataa
    57901 ccaatgcttt acttgcagga gtggtggaag taggtgttac cagaccatag atatatccag
    57961 tcttttgatt aaccccacta agctcaaaat tatctataaa ccatcctatt cctgtggttg
    58021 cactatcaga tgtcatacga aagcgaaaac gcacagtttg acccgcatac tgactcaaat
    58081 tgaactgcac cgtttgccag gagtttatag aacctgtaaa gcctggctga ctatttaaac
    58141 tgacaatact attatgagga tagccaccaa gaggtgtaat aactgtccaa ttatttccat
    58201 tatcaacaga aatacataca ttcccaccat cataatagtt ttccatcccg tagatatgat
    58261 gaaaagttag gatgcaacta ctttccaaag tatattcagg agtaatcaaa acataatcaa
    58321 tgagattagg atagttacca ctcaaattag ttgcccagac ttttactcca gaatagggtc
    58381 caacttgagc tggaattccc cattcccatc cggtttcaga gataaaatta ccatcattag
    58441 attcaaaatc attaaataca ttagcaatat tgtaaggtat tagaagattg ttagatatgg
    58501 gcaatccatt agatatagat agattaaact gcatagaaat atattgggaa agtgaactta
    58561 cacccgtaaa ttgaacctca aatacaaatt gcattatggt atttggttct atcattggta
    58621 aactaattat gggattgaca atcatcacat ctgaactgga ggttgatagc gttgccataa
    58681 cctcacgagc ttgcacatct gaattattgt ggacatttac aatcaattta accgtttcca
    58741 gtggatcaat aatcccattg aaattggcat ctgcatcgtt aatcagataa gatacgaaca
    58801 tcatcacaga ggcttctact ctaatgctga aattgcgttg ccagctaaag tcaccagaat
    58861 taatctggag tcggaagtta atcaccgtgc cattaggaca ttctggtgcg atggtaaaca
    58921 caaagggctg ttgattcact ccataactat ctgtggttag gggataataa gttcctgtgg
    58981 cagtgattat gttgacatag ggattgttag taattagagt agcagtcaca ttctctgctg
    59041 tttgatttcc tatattctga agtttaatac ctaaatttat ggtttctcct ggttcgcaga
    59101 caccattgcc attgctatca tctatataag tctcacaaag tataatatgc gggtcataat
    59161 aataaagagg aacattcgtt atataaagtg ccctttgatt tcccagaggt gaagcagctg
    59221 tgggataaac attattgaaa gtatattcta aacccactgt tcctgaatgg tcttcaatac
    59281 caatagtagc atagcaacca tgacgattac cagtactcat atccacatta ttgaaggttt
    59341 gatattgaaa cttaatagga ccatctccca gggaagtagg atacatactc tggtcataaa
    59401 gtataacttg aaaggtttcc agtgaagaac cgttagatcc atttttaaga ttataccatt
    59461 ctatcacaaa gcaatgatta ttacggtcat accaggtgta gattccacca cctgaatggg
    59521 tggctaaatc atcccagaaa ggtgcaatca tcggacttgg tcccataggt ccagggagac
    59581 gataattgcg aaactcagca ttttcggtca ctcccatagc aataaaaccg tttgaacaaa
    59641 ctgttatctg agtatagata cgaccataaa actggaaggg aaaaggtaaa tctaccactt
    59701 ccagagcatc agaacccacc tgatctcctt cattgctact tgtataggaa tcactaatag
    59761 ccagagctgt ccccaatcca ccttcagctg gagcaatacc tatccagtta aacacaggac
    59821 attcttcata gcctgtatcc tgatcatcat aaataacata tccataacca tcaggtccga
    59881 gaggagcgtt tacggttacc tcacctatag taagagaaaa atccaaccat tgttcaaaac
    59941 cgaagctatt ataaagcttt aatcgcattg gaataatcat tcctggaagc aacaacggtc
    60001 taccatagag ctgaaagata tcggtggttg gagttacttc aatatttgga aataggtcac
    60061 cataaaaggc tgtatggtct gtgatattta tcaggtcatt taaagtatat agacgaccat
    60121 agatatcagt tacgggaata gtagtttggt tagtcacggt aacagtaaat ccagcatttt
    60181 cacttgggtc caggactcca tttccaccat ctatcacctg ataactatta aaaatcattt
    60241 ccgcattatt cactttaaca aattctgaaa cattatagct aatattatta ctatcagtaa
    60301 gtataagatg aaagcgcagc atcatatcat tgggtgtatt ataagcaata tggattacca
    60361 caggattatt attaaaacca agggaggaag gagtaatttc cccaaaagta agcagagaat
    60421 cagttattgt gacataggga ctgttgcaag ttaagtatcc agatatacca gagatggagc
    60481 tggtagaagt attcctgagt ccaaagagaa attcaattgt ttctccccca tcaactaatc
    60541 catctccatt accttgagag aagtctatat tatcatcatc tactatgata gctccaggaa
    60601 caagagtgcc agaattgtct acagctatag attgctgtaa tggcttaaac tcatgtttag
    60661 acacagtcag agtgcaagga ttaaccgtca tatttgaggg caaaataagg ataatattac
    60721 catcctcatc agtatagcca cgactgataa taacattgtt ctgcgttaaa gttacacaag
    60781 catctttaac tggctcacca gtgccatctg taacaaatac atccattaaa ctcagaccga
    60841 gaggaatagt ggttatacta ttgatattaa aatgattagg gataccacaa aatatttcca
    60901 tagtgggatc acccatcaga tttaaccaat gtgcaaaatt attagcatta gaaggagaag
    60961 aaactccaaa tatctgattg ataaacaact taccatttag catagcttca ccaggtgttc
    61021 gcatattata ttgtaagatc ccaccccaga ctccaccatg aagagcatta ttgaatgtgg
    61081 tatgggtact ggaagtagcc atacccatag cggtaacagc acccttaggt tgtgcagagg
    61141 tccctaaacg tatataagat tcagtggtag cggtagagct aaagtttccg gtattgcagg
    61201 taattattat cgcatgagga agtttatagc cattgtttaa agaatcagaa ggagaccatc
    61261 cactcatatt aatatatcca cgataactgt gaaatcctat tcctgtatta atagcgctgt
    61321 tggctaaact tggagaagga tcgtcaccat ataattctgt aaaagtataa tcaggattaa
    61381 tatttagggc acattctttg atatatttac tggtataaac aacactgact ccggaaggtg
    61441 aataatcacc tactaaaagc attctattta gccattgagc attattaaga ttaatatctc
    61501 tttcgtagag atagatttta gcaaagatgg tctgaagttg agaaagattt tctgcactga
    61561 ttctaccaat gtatacatca cccaataaat cattacccgc taaatgagta taaggataat
    61621 ctgttgcccc attggaatag gtaaaacaag gaatggtata ggagcctccg gtatcaccta
    61681 taagaatcac ataatctggt ctggtagcag gattattata ggcattttga atataggact
    61741 ttatagaagt agtgctactt cctgcttcag tactggctgt acttgcaagt gaaacatctg
    61801 ctcccttctg tcttttccag agggcaaagg aatttataga agaaatataa ttggtatcag
    61861 agttataacc ataaatgatt aaataacgag ggggaatatg agtatctatc aggtcccgat
    61921 aatcatcaaa attgaatata atggactcat agatattagc aaaagcggga gataaggatt
    61981 gtaattctcc ttccagttca ttgattcctg gctcatttaa ataattaacc cgaaaagata
    62041 tagattgatt tatggtaaga gtatgactct gtggattata actaaaagga ttgacctgaa
    62101 ttccgataat acggaaatcc ctcaatatca ttggatcact tacttgaata agattagcag
    62161 gataggaagc gccattttca taataagcac tatcaataac aaaagacttg ggagattcaa
    62221 gttcctgtcc ttgttgtact ggataaggta gaaattgagg tagcacctga gtttgagtgt
    62281 tcaaagtttc aatctgaacc ttgccttgac gaggaatagc cagcattatg tttatagttg
    62341 gaagctcagg taatccacta tccatggtta ccccagcttc tggtatcaat attctctggt
    62401 agacattgcc cccagcttct tcttctatga tttcatagga aggaaggttg aattgaatat
    62461 ccatatggtc tatagctttc gtggtaatct ggaaagcttt attagtttga gaaataacat
    62521 tatcgtttaa agctaagata gcactgagac ttaatcccag cataataata aggaatactt
    62581 ttttaatcat taagtcacct ttaattgtgg gaatgtttat acttgaaaga agcaataatt
    62641 attccgtttt tttataaatt ataatagagg atatattcat agggatggat acgattgtta
    62701 acttcgtcat tttcttttag tttaagttta atataggatt caattaattc ctcgttaaat
    62761 acatcaccct caagtagata gtgatagtct tctttcagac tttgcatagc ttctttcaga
    62821 ttagctggaa tagagagaag ttttgatttt ttctcttcac tccattcaaa aacattgtcg
    62881 tcataaggac ctaagttata ttttgcgggg tcaatttgat ttttaattcc atccaatcca
    62941 gccattaaca tagcactcat agcaagataa ggattgcaag tggcatcacc agtgcggaat
    63001 tcaaagcgtt ttgtttcagg tgaattagca tatctgggaa tacggatagc ggcactgcga
    63061 ttggcaagtc cataaaatag ttttactggt gcttcaaaac ctggcagtaa acgcttatag
    63121 ctattagtgc ttggattggt aaaagctaca agagatcttc catggtataa aattccacca
    63181 ataaaccaga tagcgaggtc ggaaaggtca gcataaccac ctttttgata aaatatattt
    63241 gtgccatcct tatgcagcat tatatgaaaa tgcattccac ttcctgcttg attataaatg
    63301 ggtttaggca taaaagttgc ggttaatcca tagctcaggg cagtgcgacg aatgatatct
    63361 ttcatcacca tcacatcatc acaaatcttg ggaaattcca gaagttctgt ttcaatttct
    63421 tgttgagaag ctaaacctac ttcatggtga tgatatcgga ctttgatacc ataattttcc
    63481 atacggtcta ccatctcctg acggatttca tagaactgat caaagggtgt gtccatatga
    63541 taacctttat ggtcctgttg tgccaatcca tcattatctt cattatcttc tggtaaggct
    63601 tctttgtgtt cagaagattc taccaaaaaa ccacaattat attcactgct ctgatatttc
    63661 actgaatcaa aaagatagaa ttccagttca ggaatccagg tgcttttatc tgcaatacca
    63721 gtggaaagaa gatattcttg tgctcttaaa gctacactgc gagaatcttt tttaacaccc
    63781 tgacgggttt ctgcatcaca aatagagcaa atcagacgca ttgtgtagtt atcatagaat
    63841 gggtctattt gagctgtttc caggtcgggc atcaaaatca tatcaccaga ttctactcct
    63901 ttcattcctg gaatagaaga gccatcaaaa ggtacaccag agtccagaac ttcttttaaa
    63961 ccgcgagcag gaatggtgat atgataccat tttccatcca taccgcagta ttttaagtct
    64021 actgcctgaa tttctttttc tcttataaaa tccttaattt tttctttcgt cattttatcc
    64081 tctcctttta aaaaataccg ccaacaccta atagttgcat agtgaaattg ataggaggta
    64141 taatcagtct accaataata ttaaaaccta aaagtccaga aacaagaagt atgaaaatta
    64201 tggttatcat accaatatgt tcttgtgcca taaacttata aaatgcagca tcaggaagta
    64261 gcataccaag aaccttagag ccatcaagag gcggaatcgg aataagattg aaaaacgcaa
    64321 gtaataaatt gaaaaaaacc accatttgac agatataagg tataatctgt tgcccttgag
    64381 tcaaatgaaa gattaaagca aatactatgg ctatcaagat attagctacc ggtcctgcta
    64441 atgccgaaag acctgtccct actttgatat ttttataatt atagggattt aagggaactg
    64501 gcttggcata accaaggata attcccatag taaagtaagt caaaagcgga agaataatag
    64561 tgccaaaaat atctatatgt ttgaaaggat ttagacttaa tcttcccatc attttagctg
    64621 tgtcatcacc ctgccagtaa gaaaccagag catggctaat ttcatgcaag ataatggaat
    64681 agaatataat gagaataatt ataagggtta gagtattttt aataaaactc attgatatag
    64741 cagcataatt cattgtttcc tcaatcttaa aaactttctt ttccccacac gtaaaaccat
    64801 tccctcttta atttcaattt ctgcatcagt ggaagtaatt ttttcaccat caatgctaac
    64861 accaccagct tgaatcaatc ttttagcttc acttcctgat tcacacaatc cactttcagt
    64921 taaaagttta gcaatacgca taacggaatt attaactttg tattcaggca tttcatcagg
    64981 taattccctt tgtgaaaaga ccttttcaaa agctctttga gcttcatcgg ctgctttatt
    65041 accatgataa aatgccacta ttctgtgtgc cagctgtttc ttaagtatca ttggattagt
    65101 gccactttcc aattcttttt gcaccttttc caattcttct tcatcagccc aagcagcata
    65161 tttataataa tttaatatta agttatcagg tatggacata atctttccaa atttttcctc
    65221 tggcggatca aaaacagcaa tgtaattatt caaggattta cccattttat taacaccatc
    65281 cagacctatc aaaagaggag tcagaattgc aacttgttct tcttgtccaa gaaagcgttg
    65341 catatctctt ccacaaagga tattaaattt ctgttcagtt gcaccgagtt caacatcact
    65401 ttgcactgca actgaatcgt aagcttgcaa gagaggatac attaattcat gaagaccgag
    65461 cggaagtccc tgttcataac gcgttcggaa ggtatcatgt gccataaatt gtgccagagt
    65521 aaaatttccc atcaacttaa ttacatcttc aacagtcatt ttaccgaacc attcactttg
    65581 ccatctaact tccgtttgtt cctgttttaa aaccgtatag agctgttcca taaacctttc
    65641 tgcattggca agaacttgct gatgagaaag tggagggcgg gtttcatcac gacctgtggg
    65701 gtcaccaatt tgggcagtaa aatcgccgat aataattacc ccaatatgac ctaagtcctg
    65761 aaaatcccgc attttacgaa taggcactaa atgacctaaa tgaacatcgg tgccagttgg
    65821 gtctatacca aattttattc ttaagggttt accggtctct ttgctgcgga taagtttcct
    65881 tactaaatct tcttctggaa tgatttcatc caccgctttt cttataattt tcaattcttt
    65941 ttctaagttc atattgtcct taaaactatg tgctttatct attttatttc acaaaatcgg
    66001 acactgctta tttgtcaagc cctactggtc taaattagat taatacttta tatagtatgg
    66061 tctaagaatt cccatccctc tataggggtt aataaaccat cctcaatcgc tgctttaacc
    66121 gacctacagg gaagattctt actgattatt tcctctttag taatagtttt caaatctacc
    66181 tgttctggat atttcgcttg catattccac ataagcttta caaatagagg ataccaggac
    66241 ggaatcctca ggttttcatc tttctgtctc aacaaagttg tgtaaatttg aaaatcactt
    66301 ttatacgcag gtgaaggatt tagagccttt aaattgagac taatttgttt gtataaatca
    66361 ttttgtggct gatgtggcat ttggggttta cgacgaataa tcgtgcaatg cagattcgga
    66421 tgatagtatt gtatatactt ttctgaatct ataactggcg gtgttttacc tcttttaata
    66481 ttagctttca tagtttatat ctccttacct gtaacctcat cttcaattac tcagtttctg
    66541 tcaatcagtt cttaatcaag cgttaatcaa gtctttatat attaacgctt gattaacact
    66601 tgataaacac ttgatagaag gaagtagata agaagaagat tatggttttg aagtggatag
    66661 attggttcat attatgggat aaatttgttt ttttggagca aatggatagg gggtttagtt
    66721 aataacatca ttttaaagct tatccatcag atttattaat gtattagagg atagaatgtt
    66781 caggataagt tataagccaa tattttgcca gagcaaaagn nnnnnnnnnn nnnnnnnnnn
    66841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    66901 nnnnnnnnnn nnnnnnnnnt agaggataga atgttcagga taagttataa gccaatattt
    66961 tgccagagca aaagaataag aagaaagaaa ttaaagagat attatagaaa gagctatggc
    67021 tatttaatta atttgagata tggtagatat tggtatcact ttcctattat ctaatctctt
    67081 tttaatcata cttaaccttt attttccgta aaacaggata atttttttaa aatttatttt
    67141 aaaagattgg taatagagaa gatagataga ttaatagttt tgaaaatata gttatttata
    67201 caagcgagct gcaatctctg atagacaatg atgtctgcca ccttattatt atatggaaag
    67261 atgaggatat ttttcctcct ctggtaggtc aatatcctca ttgaccccaa gcaactaaag
    67321 ctacaagctt atctcatagc aagtgaaagc ttaataagct gaaaactcat ttttaatatg
    67381 aaagccctaa cgggcttgga aggatgagga catccttcca cctataaaaa tagccggtta
    67441 tagaaaaacc ggctatatat tatatgtttt aaggttaaat actttagttt aatcttcttt
    67501 ttcctgacac aattctatga ggacaccgag agttgattta ggatgcagaa aagcaatttc
    67561 agaattatga gcaccgggtc ttggttgagt atctatgagt cggaacccat tttcatgtgc
    67621 tttgttcagt tcttccgtta cattatctac tgccagagca atatgttgta ttccttcccc
    67681 tctattttca ataaatttag cgataggact ttcaggagaa gttggcatta ataattccaa
    67741 tcgggtttct ccacaaggga agaaggctac tttaaccatt tgagaaggga cttcttcaat
    67801 accttccagc tggagtccga gtttttcata gatggcaata gcttcatcca gatttttcac
    67861 tgcaataccg atatgggaga tatgtttaat cataaaatcc tctatttctc ataatttttg
    67921 gctaagtcaa ttcccaattt catagctcgc agattgaggt ctataaaggc aggtttcaca
    67981 ctttcttcta tagctttttg caagaaagct attttcacaa tatccgtaac tttaaccaga
    68041 aaagacagcg ctataatatt agtaggaata ggactgccca gtttatcaat agcaatctct
    68101 gtgaagggaa cttcataggt attttcaacc actaaggcga tatttttaac aaaggtagta
    68161 tccacaatga ggataccatt ttcacgcagg tcaaagagat atttatcaca agattcttga
    68221 ttgagcgcaa gcaggcaatc aaaagatacc gcttcgggga aatatatctc ttcattgcta
    68281 ataataacat cagcgtaaga atatccacct cgtgattccg gtccatagct ctgagtttga
    68341 gtaacatgat gattttccag aactgcggct cgtgcgagaa ttatccctgc cagaatcaat
    68401 ccctgaccac cacttccaga aagacgtatt tcatagggat atttcatttt gaacctccct
    68461 gtgcttgagc ctttttcatc aattcagcat attgagcaca gtattcaggt ctaatatctt
    68521 tatgcaagac gcctcgtata atctttccct gcactttttc aggaggaagt ttatccactg
    68581 cagcaagcgg tatagagtta tctctcattt ccttcatcat ttctggagcc ccaccttttt
    68641 tattcaatct accataaatc acaggacaag cgcagataac ttcaattagc gcaaagcccg
    68701 gatgttctat cgcttctttg accatatttt gagtttcctg cgtatgaaaa gccgtggttc
    68761 tcgccacata tgtagctcct gcacccattg ccagtttgca aacatcaaaa tcaggttcta
    68821 tattaccata aggagcggtg gtagcataag cctcatgagg agtagttggt gaaacctgac
    68881 ctccggtcat tccatagata ttattattga gcataatcac tgtcaggtct atatttcttc
    68941 tcgcagcatg gatgagatga tttccaccaa tggcagcaca atctccatcc ccagtaacca
    69001 cgatgacttt aagatgcggt ttatggagtt taaccccggt agcaaaagca atagctcttc
    69061 catgcaaagt atgaagagtg ttgaaatcta cataaactgg cattctggaa gaacaaccaa
    69121 ttccagagac cataacgata tcattccggt ccagattcat tcctgcaatg gcacgaataa
    69181 tagaaccaag cactatccca ttggaacaac cagcacacca cacatgaggg aatttcttat
    69241 cgtgacgcag atattcatga ataatttttt gaggtatagc aggcatatta tattacctcc
    69301 ttaatttttt gcaggatatc ctggggcgga ataagtttgc tatccactcg ttgaatcttt
    69361 atcagtttgc tatcactacg gtctttaact aagcgctcaa tttcccgaat taactgtccc
    69421 tgattcaatt caggcacgat aacaatatcc acattcctta acattttacg cactgcatca
    69481 tccgggaagg gccagattgt taaaggacga agcaaacctg cgggaatccc ttcctttctt
    69541 gccataacta ctgctgcttt agcagaacga gcagtaatac cagcagcaaa aacagcaatt
    69601 tttgcatcat ccatttgaaa gctttcaatc tggacgaggt cacggatatt ataagttatc
    69661 tttttcttaa gtcttgccat catttctgca gcttcattgg gcttattagt aggaaacccg
    69721 agagaatcat gagtgagccc agttacatga aagcgatatc cttctccgaa actggctaag
    69781 gggatgggat atttttcatt atcctcatag tgcttatacc agtgaggtgg cacagtaggt
    69841 tttactcttg agataacacg cacatttgac aaatcaggaa gtctgactgc ttccctcata
    69901 tgaccgagca cctcatccag taagagtatt acgcaagtgc ggtatttttc ggaaagatta
    69961 atagcacgaa tggtgagttc atagctttct ttaacgctat ctggataaag cacaatggct
    70021 ggactatcac catgagtgcc ccagcgtgat tgttgaatgt caccttgagc acagctggtt
    70081 ggcattccag tggaaggacc aacacgttga acatctacta ccacgcaagg agtttccgcg
    70141 cttctggcaa agcctatgcc ttcctgcatc aaggaaaaac caggaccaga ggtagcagtt
    70201 aaagctttcg ctcctgccag tgatgctcca atcacggcac agatacttcc caattcatct
    70261 tccatctgga tgaagactcc gcctcgtttg ggaagctctg ctgccaggat ttctgctatt
    70321 tctgtggaag gggttattgg ataaccggca aagaaattac aacctgcatc cagagcacca
    70381 taggcaacag cttcattacc ctgcatcagt acagtctttc ttcccttgag gttctgcaat
    70441 tcttccactt tagtggtaga actagtttta ttctcattat tcatagtcgt atttggattt
    70501 attgtttcag aattagacat ttggactcct taatgttctt tagcaccagt gatagcaaaa
    70561 tcagggcaaa gaaggtcaca tgtctcacag tgagtgcatt tttctggtgc taaaacatag
    70621 ggtgaaccat cggcttttct tcctaaacag ccggtaggac aaaaagcaac acagattcca
    70681 cacttcttac accagttgta ataaataatt acaggggagg atttatcacc agattcttta
    70741 atttctggga gttcaacttc catcttttct ggagcatctt cctctcggat aatcatgcct
    70801 atacggtctt tttcgacttt tttaggcatt atacctccca atttgtgatt tagcttgttt
    70861 atattgcatt ttcaagattt ttccgcaata acctttttga agaaggtgcc taaacgacga
    70921 caaccttcac ggttattctc ttccgattca taggagaagt tcagacgcat tgtattctgt
    70981 cccttaccgt tacagaagaa agatgtccct gccacataag ctacattttg ttctttgata
    71041 caatcaagaa gcagagcatc agtatcaatc cattcaggac cagtagccat taagaaaagt
    71101 ccaccctgag gttttgtcca agtaaattct tccggcataa actcttctaa tgattccagc
    71161 ataaccttct gtttggattt atacatattg cagatttcag caatcttagg atctaaataa
    71221 cctctttcca tataacgagc tgcaatgcgc tgagtgaaag gaggagtgca aaggtcagta
    71281 gcttgtttac caatcaccac tttatccagg acatcaggat gtcccataac ccatccgata
    71341 cggaagccag gacagaaaat tttggagaaa gtgcctaaaa ggactacctg ttgagttcca
    71401 tccagctcat aaagagtgcg ttgattttca ccttcgtatc gtaattcacg ataagggcta
    71461 tcctcaacga taagaatatc gtagcgatga gcaatatcaa tgatttcttt acgacgcttt
    71521 tcactcatag tcatcccggc aggattctgg aaatcaggaa tgacatagat gaatttaggt
    71581 tttttgcctc gagctatggc ttttttaatt tcatcttcca taacttctgg aatttcacct
    71641 tcatcatcca taggaattcc aatcatatcg gcaccgtagg aattaaaagc actaagagca
    71701 cccagatagg aaggtagacc cactataaca gtatctccgc ggtcaataaa gcttttggct
    71761 atcaggtcta aagcttgttg agaagcagta gtaataataa tattatccat attcacatca
    71821 ataccaaatt tcttatagcg atcagctaac tgtttccgta aaagattatc tccttcggta
    71881 gtgccatact gaagagcgta ataaggttca ttttccatca cttcagcaat aatacccttc
    71941 agctcttcca caggaaaaga ttgaggggca ggaaagcctc cagaaaagga gatcaagcca
    72001 ggctgattta gatactttaa taattcgcgg atggcggaac gcttcattcc gcgaatattt
    72061 actgacagga tgtcttgcag atcgttaatc atttcgatcc tccatctatt ttattttgtt
    72121 tataaactaa aactttttta ttttttttcc gccattcttt ataagcattt ttgatttcca
    72181 gctcccagac cagacgttta tcgttatctt caaaagtgat aatatctata taatacatac
    72241 gatgattctt gcctacaaag ctctttttgg gaaattctac tactatttca ccttcaatat
    72301 tgagtatggt aatttcattt aggaaaacac catccgcaat ttccagaaca gcacgagctt
    72361 ccacactgcc agtctgaaca tcacgaaaat gtatattcat tcctgctcct taatttgata
    72421 aataaactat taaaaaaaga tagatttatg gtcaaggact tttttaaacc ctttttcaaa
    72481 gggcattcag attagacccg caagaatgtc ttctacggtt acctcgccaa aaaattcatt
    72541 caaaggaagc gattccagag cattgcgaag ctcttcggtg cgatgcggaa gtcctatcaa
    72601 agctgattcc aattcagaag gatcccgttc tccaaagaaa tctccataaa tccgtaaacc
    72661 tgtaatttta cccttatgca catcaatatg aaattctatt gtccctgcag aacatcttat
    72721 actctgttta tgattgtaag ctggagaatg tccaaaattc cattcccagc tctcatattt
    72781 actcttcatc aaagtgttaa tccctttttt atcatcctca gttaagtgat attctcgtgc
    72841 atctggatag agtttaagca cttctttctg aataagctgg ataaattcct ccaggcttat
    72901 gggttttgtt agatgttctg aaatattagt aacccttgct gccacagaat gtacggcttt
    72961 atctttgaac tttaaaggat tggcttttaa ggcagcactg agatctgtaa tctttgaaga
    73021 aaagaggata gtgccatgct gatgaatctt atttggatat accgctctgg cattgccgga
    73081 aaatttcata cctttaatag tcaggtcatt cctaccttcc aactttgcct caattcccaa
    73141 atctttcaag gctgcaagaa taggtgcagt atatttagca aaaccacgat cttcatctcc
    73201 agtgcgattc atcaaaaaac aataatttag atttcccaga tcatgaaaaa ccgttcctcc
    73261 tccagttaaa cgacgcacca ccggaatatt atgttccttc acataatcca aatttatttc
    73321 tgctaaagta ttttgatgtc tacctataat gattgaggca tcattacgat aaagaagaaa
    73381 tacatcctca ggaaaattat tcaagatata ctcttcacag gcaatattga aaggagcata
    73441 atgacttggg ctattaataa ataacataag atacttcttt ttctccccga taggtattaa
    73501 tcattgtttt aaggttcaac ttattgatat tcagcaaaat aacctattga acctgattta
    73561 attaaaccaa aattgctaat tgataaagta tgtcaatcca gatgtttttg agtaggtgta
    73621 actattacct gctaaaataa ggatttagtg aacccattaa taccttatta atttataaag
    73681 ataggtattt tccttgttca cataaatttt aaatattaag cgtctctcta aaaaaaagat
    73741 ttctgccact atccaggtct tgaaattgca ctatctcata aggaaagcca tattgattaa
    73801 ttttagcttg agcactgtcc agatttaaat taagaaaatc tactactacc tcttcccatt
    73861 gattcaacat tgtatcagta ggttgaatat aattcccaaa attattattc tgaacttccc
    73921 agggagcata aacattagtg ttagttaaaa ttccttcagc aatatgacta gtccagttat
    73981 catactcaca agcaggttct gaaccataga aaaaaccacg gaatgatcct acttctgtaa
    74041 caatagcatt taatccccaa cataaagaaa atgataacat aataagaaga aatcgtttca
    74101 ttttatcctc ccctatctaa aaataaagca ataattattc cttagtgtaa aaaaatcgtt
    74161 ccttatctat cttgaaattg cagtttagga taaacatcct cagcactttt ctagttgcca
    74221 atctatagct ttgctttaat ttgtaagtgt agctttattt tttattggat gcatatagga
    74281 aaggagaaaa aatggaatct tttagctttt ataatccaac acgagtactt tttggaaaag
    74341 gtcaaatagg aaaaatagga aaagaactta aaaaagctga tattaagagc tgtttaatga
    74401 ttgctggagg gggttccatc cgtaaaaatg gagtttatga acaggtttgt gaaagtttaa
    74461 aagaaaataa gattgaatgg atagaaagct ggggtgtgca acccaatcct actgtagaaa
    74521 aagtgcgtga aatgataact ttagcaaaag aatccagtgt tgatgccata ttggctgtag
    74581 gtggaggtag tgttatagat agtggtaagg caactgcagc agggatcttt cttaatgata
    74641 tctggaatgc ctatgtggac aaagaaaaaa tcatccgtgc tctacctatt tttactatcc
    74701 ttacaatctc tggaactgga agcgagatga atggaaattc ggtcttaacc ttaagttctg
    74761 aaaagaaaaa atggagtatc tcatctaaac ttctctatcc taaggtttct attatagatc
    74821 cttctattca ggctactctc tcttttcgac aaacagtaaa tggggcaata gatgccattt
    74881 ctcatatctt agaatatata tttatgaatg aaactgctat tgttacaaga aacattgatg
    74941 caggtttgat aaaaaccatc gtaaaaatga cggatagatt acaacaagtt gccaatgatt
    75001 atacagctcg ggcaaatctt gcttggtctg ctactttagc attaaatgga attagtggag
    75061 taggattaca aggaggagat tgggcatgtc attccataga gcatgctctt tctgccattg
    75121 atcctaaaat tgcacacgga gaagggttag gggttatttt cccggcttgg attgaatatg
    75181 tcagtgaaag agagccgaac cgtttttcct ggtgggtaaa agaagtctgt gaagaagata
    75241 gtgtagcacg tgctcttcga aaatgccgtg aaaccttgaa acgctggggt tctgctacaa
    75301 accttagaga attgggaatc agggaatcac aactacctga aataagagat ttgcttctga
    75361 cttatggtcc aataggagaa attgttcgtt ttagggcaga ggatattgaa gctctcttaa
    75421 tgcttgctta ttgaaattgt attaatatac tacttgacaa aagaactcaa agaaaaaatt
    75481 aagaaagcta aattattagc tgaaggttta aatgaaggaa caaaatagag taaaaagcaa
    75541 actaaaaaag cagccttata aagaaccaac ttataccatt accccaattc caccggggaa
    75601 aaaattccat tgtcgtaaac cctggttaca ggattggatt gaagccatac tttttgcttt
    75661 tgtggtggca atgataatcc gtaactacac ctttcagaac tttatgattc cttcctcttc
    75721 tatggaaaaa accttgctca ctggcgatta tattgtagca aataaaatta aatactatat
    75781 agaagacccg aaaagagaag atattgtcac tttccgttat cccaaaattg aagaaggaac
    75841 accggaacat ccagattata aagataattt tatcaaagta ttccctccta tctatatcaa
    75901 taaaaaagca gccttcagca agcatcctat gacctttatg catattgcct attatgcaag
    75961 aaagaatata gttaaaagag ttataggtat gcctggagat attatagaga ttaaaaataa
    76021 aaaggtttat ataaatggtg aactctttga aagaggttat gaatgttatg gcatagctta
    76081 tcctccacca cctcaacctc cttttcaaat tatagaacaa acttttgctg aagatgagga
    76141 agatttatat atatctaaca gcgattggta taaaccttat attgtccctc ctgatagcac
    76201 tagaggaatt tcagccagaa aagtctttaa tcgtgattgg tttggaccta tcaaagttcc
    76261 agaaggacat tattttgtga tgggagataa tcgtgatgtt agtgaggaca gtcgttactg
    76321 gggatttctt aagaggtcag atataactgg tagtccctgg ttaatattct tttcacgagg
    76381 aatagagtat aataaacttt atgatactcc tcatatccga tggaacagga tatttcgtct
    76441 cgccagatag ctgtttatct acatatcccc ttttgcttaa ctcattgcgg atattgcact
    76501 tttttcagtg tcccctattc aaaatcttca ctggaacttt atctacaata tctgcataaa
    76561 gaaatagacc tattttttca atatcatcct gatttagctt acgcaaagtc tatctatttc
    76621 ggaggcggta ctccatcttt attaagagca gaggatattg cagaactctg tgctcgtttt
    76681 aattttgctg aagaagctga aattactctg gaaataaatc ccctgcaaat aactaagaac
    76741 tacctctcag ctttgaaaaa cacaccagtc aaccgtcttt ctattggtat tcaaagtatg
    76801 gataatgaag aactaaatct tcttgataga aaacataaag ctgagcagat aaaggataaa
    76861 attgagctct gccgtcagtt cgggtttaat aatatctctc tggaccttat atatggttta
    76921 cccggttctt ctcttaataa attaaaagat aatattatcc aatttctata tttgaaacct
    76981 gaacacattt catgctatct tttaaccctg gactcggata ctcctttagc ggcaaaaata
    77041 aagaaaggga aagtccctcg tttaccagaa gatagcttgc tggcagaaga atacgaactt
    77101 atatgttcag aactggatag ggcaggatat gagcaatatg agatttccaa tttctgtctt
    77161 cctggcaaac aatcaaaaca taatctgtgc tactggaaaa gtgagccata tcttggatta
    77221 ggagcttcag ctgctggatt cttacctccc tatcgttata ccaatccttc taatctgtat
    77281 gattactatg accatctgga taaaggatta attatgcctg aggctgaatc ctgttcctcc
    77341 aagcagatga ttgcagattt cattatgatg ggattaagat tgacagaggg tattgacctg
    77401 aatgagctga aaaaccactt taactatgat ctggaagaag ataagcaatc acaactggaa
    77461 aagttatata gtctgggtat gattgaaaaa aatggctctt atctctctct tagcactaaa
    77521 gcactttttg tctctaatac catcattgga gagctaatat gacgctaagg gaacttagag
    77581 aattacttgc cagagccatt aaagataccg aatttgaaaa tcatacttac tttgcaggtg
    77641 gttgcgtgcg ggacttctta ttaaatccag atgctgctat tgttgaagca gatatagctg
    77701 tagaacttcc agaaggtggc ataaaacttg cttatttttt tcaggaaaaa ttcaatactc
    77761 ctgctcctgt aatccacaaa agctttggaa ctgctaaaat aatcatagaa ggtataaaaa
    77821 tagaaacggt gatgacccga agcgagaaat atcctcctgg tagaagacat ccttatgtac
    77881 agtttgcaga cctgtatacc gattgtctac gtcgtgattt tactatcaat gcactatatc
    77941 aagaaataat gacgggcaag gttttagacc cctgtcaaaa aggcttcgct gatttgcaag
    78001 ccagagttat tcgctgtgtt caggaccctt ttcattcttt cgtagaagac cctctaagaa
    78061 ttctgcgtgc tcttcgcttt gcggtcacct tagattttga aataacagct gaaacacttt
    78121 ctgctattaa gaccaatgct catttagtct caactttaag tgccaatcgc tgtgctgacg
    78181 aatattctcg cataaaacaa cttcccgcag aaaaaatagc tcagtggaag aaactgcttg
    78241 aagagactgg cgtcctaaat tatttaacag ccaagattcc cattcggtag gatattctaa
    78301 ttcttgacca aatcgctatc tacagcataa atataatatt tttggaatag aatccaattt
    78361 tattataata tttttggatt tgattccaat tttattataa ttttgcctct tacagtcctt
    78421 cccaatttat taactcttct tttatatcat aaatcttatt tttggagaac tacgcaaaaa
    78481 caagagatag attat
//
LOCUS       DAORZF010000004        70436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_331_length_70436_cov_339.649536, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000004 DAORZF010000000
VERSION     DAORZF010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 70436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 70436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70436
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_331_length_70436_cov_339.649536"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(684..1844)
                     /locus_tag="PK015_01160"
     CDS             complement(684..1844)
                     /locus_tag="PK015_01160"
                     /inference="COORDINATES: protein motif:HMM:NF025840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DarT ssDNA thymidine ADP-ribosyltransferase
                     family protein"
                     /protein_id="HOL33115.1"
                     /translation="MLKLRELYYITHIENLLSILKRGILCHRKIIEEQINFTPIYDED
                     IIALRKGKRVPDGRELWDFVNLYFQPRNPMLYRVLFHSNFKIDEVVVIGLKNSLLNRN
                     DIILTDGNAASSNTQFFPVKDRDISKYIREKTDKEWWAIEDGSKREIMAECLVPEKVE
                     PEYISEIYVANQDAMNRVRDICDERITVIPEPELFFLPSRQIPLTENLNLMEGDMFFS
                     RMQTLTISVNTQGVMGKGLASRAKYQFPDVYVKYQDLCKNKILTMGKPYLYKRESSLD
                     FILSDDAEKLNNLNLQTWFLLFPTKTDWRKIADLKGIEKGLQWLVNNYKVEKITSLAI
                     PALGCGLGWLPWGVVGPILCKYLKELEIRVNLYLPLEQKIPDDQLSEDFLLK"
     gene            complement(2193..2726)
                     /locus_tag="PK015_01165"
     CDS             complement(2193..2726)
                     /locus_tag="PK015_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33116.1"
                     /translation="MKYETVEMLKNLNEQYKIEFGKICQKLLALSCKRAGFDKIVERE
                     VQGVDIDASNNAGIKYAIEVKTTTDDSINIADKDIKGLADRQKDGYKPLLAALRIDAL
                     EDWIFAEAEYLNKGNNLVSYLEIYNLPELQQLIAPYFDDVVKEHYYSLLNNSNNKPLE
                     YLKKELEKSGITEVDQS"
     gene            complement(2723..4921)
                     /locus_tag="PK015_01170"
     CDS             complement(2723..4921)
                     /locus_tag="PK015_01170"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOL33117.1"
                     /translation="MLTISSLKVQGFRGYTNYQELVFDNDAVFLFGDNHKGKSSTLNA
                     IEWCLFGDESVGGTTGLRERIGWEVPNRNMDPSKETFVELTLKDEQGSEYKVLRKYIT
                     AKKDALTVTFPDNKSFTDADATSKLSSLIKLSYRDFVTTVYQRQEAIRSFLVQEPKDR
                     NDAIDRLMGLSDYKNIFDNLDYNNIPKKEKQISDVFIEVEKSLEDTLRLKKNDLDTQK
                     MNAKSLEIDEDQFNLKGLKSKAETEIFKPLTDFTQSVNLTLGEIPALETEDDFAKFKD
                     AVKKEITRLRSELPDLQTQIELNKIQIDLSNLKNSFTELKQKVDENQKSLNIFIEQNG
                     DDKAIQEKINELDKLIKDKEEEIKREDSKIASIKYAISYLKMEKVNKNICPVCGKETE
                     DLLTHLETEMKEQYDQAIEKLTQELESMKADFRKQNSLLNEYSSLIKVLNESKEKLSE
                     FYKTLSEALKREITEKDDVVVILNIQIEEIAKKLQSIKERIDSKQQNLSRIESELEPM
                     EIITEILKLTKEINDITNFEEDPTFQELKAKRNEAMKALNNIQRIGLAISEASKREAK
                     DKVERTSKIIEDYFCKITDNPAIKKIKFEINEESSRIGSNDYKFKDVSREEKGKDEKG
                     KDLIPILSQGDMNALALAIYLGMALEGAEDMPFDFIIMDDPSQSMGSHHKRNLVDILN
                     EVLKRKKVIVSTMDAELQDLLSKKITKMKTCHNFEDWTDTTGPIINKEEL"
     gene            complement(4933..5142)
                     /locus_tag="PK015_01175"
     CDS             complement(4933..5142)
                     /locus_tag="PK015_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33118.1"
                     /translation="MSYKELVEQVLKESKEKEDYKSEQIDLWNKIVEKYVAAGSVGVR
                     NMVEEYFQHSYNEYIKKIEEVKNKI"
     gene            complement(5154..5486)
                     /locus_tag="PK015_01180"
     CDS             complement(5154..5486)
                     /locus_tag="PK015_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33119.1"
                     /translation="MGSVLENFQEAAKNLHLCELIDLNGNHRIVEPYIVYSSSTGKQL
                     FHCYQISGYSKSGKSQGWKNPEINSFQEAIILDDTFLPRPEYNPFNEKLFTVVYFSIP
                     TIDGRQRH"
     gene            complement(5778..7295)
                     /locus_tag="PK015_01185"
     CDS             complement(5778..7295)
                     /locus_tag="PK015_01185"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOL33120.1"
                     /translation="MKIGKKLIPIILIAIVASCAFAKTGGTEPPQLSTSKNQSSMFVN
                     DEGINPVVKVVRDVRESVVQIKVEAKVNVPAITNPFFDDPLFKYFFPEIPREMQRKIT
                     SMGSGFIYEFNPQTREAYIMTNNHVVEKGKEGTITVTLADKINYTAEVVGLDPSTDVA
                     VIKIKVKEGEKVTIAPLGDSSKLEIGEWAIAIGNPFGDVGLDRTVTLGVISAIGRSNL
                     NFGANSPIYQDYIQTDAAINPGNSGGPLLNINGEVIGINSAITSTSGGNIGIGFAIPI
                     NLAKRVVEDLVATGKVTRAYIGILPQEITADLMEAFDLSEVSGVLVAKVEKDSPADKA
                     GIQVGDVIIEFNGEKVPNVSKFRIAVATSKPGVQVPVKVIRNKDTLTLHITLDVYPED
                     KLASTEKSTKADTNAGITVVSIDSPYARQNNIKADRGVVVSAVAPDTPAARAGIQPGY
                     IILKVGNTEVNSPEEYNAAMDNAIKTMKEKSSNTIVLYVQDRSKNEQFVVLRFNS"
     gene            7490..9616
                     /gene="recG"
                     /locus_tag="PK015_01190"
     CDS             7490..9616
                     /gene="recG"
                     /locus_tag="PK015_01190"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOL33121.1"
                     /translation="MSFQHYVYMDKKDIKSYIKDTSTPVKFIKGVGEARAKQLAKIGI
                     NTVMDLFEYFPRDYIHRKIEPAIFELNPGDSIAMTTMISWVNEKSTKNGKYMLNVGVS
                     DGHSTLICTWFNYPKDYSQKFKPGNLLWVSGTLSEYNGQLQLMHPEIEFVNEEEESDF
                     WKKREYLPVYPLTGNLTQKFFRRIIYNAFEEYVDKIEENLPEFVLAKYGFLPRKIALQ
                     KMHFSLHPEELDEIHKRFVYEEFFYSQLLWARHKHYHTEEIKGIRFENKRQLTAQLYN
                     KLPFKLTNAQIRVINEIFADMTSDKQMSRLLQGDVGSGKTIVTLFAMLLAVENGYQAA
                     LMAPTEILADQHYHNITRLLEGLPVKVALLKGGTYKGKEQTKTDIREGNADIIIGTHA
                     LIQREISFDKLGFVAIDEQHRFGVQQRAALARKHKHPDLLYLSATPIPRSLSLTVYGD
                     LEVSIIDELPPNRIPVTTLIRSSQKIDLVYKEVAKELEKGRQVYIVCPLIEESDKVDL
                     LDAQRMHHYISTKIFPQYSCILIHGKMSSKEKEDIMRRFKDGEIKILVSTTVIEVGLD
                     IPNATVMIVEHAERFGLAQLHQLRGRVGRGGQQAYCYLIEHMPATNIARERLATLAST
                     TDGFLIAEKDLELRGPGEIFGTEQSGLPHFKFTNLFSDQPILKLARKDAFQIIAQDPE
                     LTNPQNSLIKDIYFRQYAEKEKLILY"
     gene            9699..12980
                     /locus_tag="PK015_01195"
     CDS             9699..12980
                     /locus_tag="PK015_01195"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOL33122.1"
                     /translation="MKRFIIIISLIVAITAIFAIKFDPDYFVSKSIIVCFTKTAIGND
                     TGKIDFTKNEGVVQTGISSFDKLAQEYRIVDMQQMHPYVKDPTWNDNGLYLQNTYRLT
                     LASDDNIDEAVEALSNDKNLIYAELEGIVRSKFVPNDPLITQQYVHDVIRSYDAWDYI
                     QGSHNVKVAITDSGVKWNHPDLRGNIWINPAEAPGMTINWDAGTISGGDGIDAGEGGN
                     KIDDLVGWDFYANDNNPIQNYASNYHGTHVAGCAGAVGNNAIGVVGTSPIVSLIICKG
                     APDSSPSQGISFAYDQVKYAGEVGADIINASWGSVGNGAYPNSIVNYVTNLGALVVTA
                     AGNENTEHNSSYQVYPADCTNAFCVASTGRNDIKSSFSDYGATIDICAPGENILSTII
                     ANNGYESNSGTSMASPIVAGVCALVKALNPELTPQQLMQRVMHTADYIYDKNPDYVGM
                     LGAGRVNAFAATMYDKIPYITIEDYSLEEIQGDGDGIPNPGETIKLKVSLNNYIDPYT
                     GLAWLDAQNLSVTLSTTYPGVTIIENTANYGTMIAGSTMWNNNQPFIFQTVGTLPSEP
                     IPFELTLTANQSADYPYNRVLPLNIPLTLNQVGWPLNFSGASTSSPIIYNLDSNSDKE
                     IVVSEPTGIVHILKPDGRTELEGFPLNLGSAIIGSLAMGDVNYDGTLEFAACLQNNNI
                     VLFNQHGTILWSVPSGGILRNGPVIASLAMDDSQQIIALTQTGNIVVLNPDGSAYPNF
                     PVSAQGICLGIPAIGDLNGDGHHEIIVATMTGNLNAINSTNAQNIPGFPVSMQGGSQN
                     PITIANLDDDNYPEIIVTTTATGNVLAYNHDGTLLFQKNLGGQIKTGAVVADVDNNGI
                     IEIIVITASGNINVLTPSGADYPNTPLSINQTVECTPVVARFDGDNYDGIIFGDSDGY
                     LHSVRVDGTESPNFPIPIGGNIKVSAALSDIDRDGDIDIVIPNDESIYVIDVKRPAQS
                     YEWYCYLGTWNRSGNIYQATPNNDYTVPQLVTTLYGNYPNPFNPETTIRFELATPGLV
                     TLDIYNQKGQLVKNLVNTEYPAGFHQIAWNGTDQKGSPVSSGIYYYHMRSGKFSATRK
                     MILMK"
     regulatory      13243..13423
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            13492..14733
                     /locus_tag="PK015_01200"
     CDS             13492..14733
                     /locus_tag="PK015_01200"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOL33123.1"
                     /translation="MKQLKITLLAVFICLFSISLYAIELFVVNSVSRTLSRIDTETGT
                     VNNTFAQLGLTPNLMDMDDNNIYVVCSGDNAIQVISRSTGNSLYFLPIQSSSNPYDVL
                     KVGDYLYVTGLFTNKLYKVSLLTNNVVSSLNVGIAPEGLCSDGSRLYVCNTGGYQFNY
                     SDSSISVIDLNSFSVIETIPVWTNPQFACIYGNYLHIACTGNWSNISGKIDIISLEDF
                     SLVQRLDIGGHPGNIWINNLGIAYVGEGMSNALYSYNASTFEIYHSAHNPLNYNAFMV
                     AGNQEIIALLNQNWTSNSIITVYDYDFNLLNTFSVGISSTDIMVAPSVSSLEDECNIV
                     PQLKIYPNPYSFGKELHFSDKIADSSQLFLYNIRGQIVFQTQIREASPIIIPNELTPG
                     IYLYRIIQGTKKDSGKLIIKN"
     gene            14812..15957
                     /locus_tag="PK015_01205"
     CDS             14812..15957
                     /locus_tag="PK015_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOL33124.1"
                     /translation="MNYFLTEEQLEMQEIARRIANEKMKPVSQKYDEEGIFPWDIVEV
                     MRQSDLFAILVGEEYGGISGKVMDLAIVTEELCAVDMGISLAFGATGLGMYPILIAGS
                     EEQKQKYLTQIAAGEKLAAFALTEANAGSDAGAIETTARKEGDHYILNGTKQWITNGG
                     EADIYCVFAMTDKTRGARGCSCFIVEKGTPGFNFGKKENKMGIRASATRELIFEDCIV
                     PAENLIGREGMGFIIAMKVFDKSRPMVGAQAVGVARGAFETAIAYSKQRQQFGKPISS
                     FQAIQFMLADMATEIEAARALVYQTARLIDSGAKDYSKESAMCKYFASDVAMKVTTDA
                     VQVLGGYGYMKEYPVEKMMRDAKILQIYEGTNQIQRGIVASALLKQY"
     gene            15981..16547
                     /locus_tag="PK015_01210"
     CDS             15981..16547
                     /locus_tag="PK015_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HOL33125.1"
                     /translation="METLAFPEHLLLHTCCAPCLIAPYHQLKEQGLSFTSFWYNPNVH
                     PVTEYRMRLQTLKTFAEKEDIPLLLHDDYGLRNFVKAVVDDIDDRCRHCFRTRLEMTA
                     QTALELGYDAFSTTLLYSIYQKHDLIKEIGEEIAEEKHIPFFYRDWRTLWSEGVRLSK
                     EAELYRQKYCGCIFSEEDRYLKRKNEQI"
     gene            16534..17451
                     /locus_tag="PK015_01215"
     CDS             16534..17451
                     /locus_tag="PK015_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33126.1"
                     /translation="MSRYNEIDLSKIHFASVQNRFSKVDTSSFAQVSDNLLDSMPDIL
                     KGAEFKEFIKRCHLARDSHKTFIIGLGGHIIKCGLAPLLIKLMEEGIAKCFAVNGSVT
                     IHDFELAYFGKTSEDVSKGLADGTFGMAEETINAINRIIIRASEEKLGYGEALGKDIL
                     ENAPNAEISLLAQAYKYNIPVTVHIALGTDITHQSPYADGKAIGDCSMRDFRIFCEQV
                     KNLNDGGVFINMGSAVIIPEVFLKALTVARNVYGTIQDFTTAVFDMNQQYRELENVVR
                     RPVEKGGKGYYFIGHHEIMIPLLVRELLR"
     gene            complement(17464..18258)
                     /locus_tag="PK015_01220"
     CDS             complement(17464..18258)
                     /locus_tag="PK015_01220"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOL33127.1"
                     /translation="MNLESLNPILLALIGTIFTWLMTAAGASLVFFVKRIQTWFMDTM
                     MGFAAGVMIAASFWSLLNPAIEMTKGNPFPAAIGFLAGGLFLFAADKLLPHEHLSEET
                     CSSEGLKTPWGESTLLFLAVTMHNFPEGLAVGVAYGAVAYGLETASLAGAIALTIGIG
                     LQNLPEGAAVSIPLRKTGLSAGRSFFWGQISGAVEPIGGVIGAALVLIAQPFLPYALS
                     FAAGAMIYAVAEEVIPRCQNGKYAHLAMLGLMLGFVVMMVLDVALG"
     gene            complement(18260..18457)
                     /locus_tag="PK015_01225"
     CDS             complement(18260..18457)
                     /locus_tag="PK015_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33128.1"
                     /translation="MKAIYTYDDEHLGKSYIVIPKIREISKVLGSVVITFDNGDKRTL
                     AVDDPNAAIQQMLEAIENFYS"
     gene            complement(18489..19550)
                     /locus_tag="PK015_01230"
     CDS             complement(18489..19550)
                     /locus_tag="PK015_01230"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOL33129.1"
                     /translation="MLSEENKQFFQNLVSAPSPSGFEGPAQKVVMDFLIPYADEINKD
                     RNGNVIALKKGSGKLKVMVVGHSDEIGFIVNHIDEQGYVYVRTLGGFDVNLLPGLRVD
                     IYHQGKPVRGIIGKKPIHMQRGTEEPPKLKLEDLWIDIGALSKEEAENKVAIGDIITY
                     HSEFEMLSDDLVVSKATDNKCGVYVAAAVMKELKDKNLKVNYYAVSSVGEETTMRGAR
                     TSAWQIEPDIAIAVDVTFTSDIPGADKRIFGDISLSKGPAITLGAAMHPAINDKLIAL
                     AKELNIPYQLEIAPGRTGTDADVIQDLKSGTAMAVIGIPNRYMHSPNEVISFKDLDAA
                     VQLIAAFITSLEDDFDFSR"
     gene            complement(19559..19954)
                     /locus_tag="PK015_01235"
     CDS             complement(19559..19954)
                     /locus_tag="PK015_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOL33130.1"
                     /translation="MKIIHFDQVPLEPVNAEGAKGAEIRWLIAQKDGAPNFAMRMFEI
                     EPGGHTPYHTHDWEHEVYVLSGTGALKTDRGELPFAEGDAIYVDPGMIHTFKNTGKDV
                     LKFLCIIPHEHPVQKKVINPFAEETANNC"
     gene            complement(20025..21155)
                     /gene="ribD"
                     /locus_tag="PK015_01240"
     CDS             complement(20025..21155)
                     /gene="ribD"
                     /locus_tag="PK015_01240"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020046386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOL33131.1"
                     /translation="MNQPRKTLKNIPTDPVNFMKEAIRLAENARGTCSPNPFVGAVIV
                     KNGKIIAGGWTQKYGKDHAEIQALKKAGKSARDADLYVTLEPCAHYGKTPPCTDAIIK
                     SGIKRVFIGIQDPNPLVNGKGRQKLLDAGIEVTSGIMQEEINRQLEYYFCRIQKGRPF
                     ILWKAALSLDGKYAAQDGSSRWITGESSRKMVHKLREEADVILTGIGTVLKDDPMLNV
                     RLPKPIKQPLRAILDPYLEIPLNSQIVRSLPEYKTAIFCARGKENSPHAEALVSLGAN
                     LYPVTANGRTLCLKEVLGILHQQGFYLVLLECGSQLASSFFSERLVDKSYIFYAPKIL
                     GGNKAILRELNLPDLNSAIHLKDITLKKTGEDWLVIGYPVYS"
     gene            complement(21115..22296)
                     /locus_tag="PK015_01245"
     CDS             complement(21115..22296)
                     /locus_tag="PK015_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33132.1"
                     /translation="MLLSKERCFRCHSERAVRKCPRYKDKLIGWKCCMNLRIDLHCPS
                     SCPYAAQFDESHTSPFPAFRSDSNTEFVQTTKKFLDYWCYKSLPELDGLSPAEFTRQD
                     SEKMLKWLEQYKYPANFPLSYLMQKLNIPYEEIAEPESPETVTFAFFDAVITQEWEKL
                     RSFTINDVDFDQFAERYTAILSSIPELKKTKSYSILYAGAADDGISAMVVLELNNKII
                     WAVLLTSATGRWKIRQNFNGGPHLFYEQNQLFQNLADALANGRAEEAWNLLTSNMPKF
                     PDCADLYYYRALYWQLAKQYNKAKEDFWNALALDNHFFAAGFALSTLYLHNSELEPAR
                     DLLNFLHQEKPDELNAWNNLAACEAGLGNLELAKNMWQEILQIAPNYEPAKKNLEKYS
                     N"
     gene            complement(22300..23067)
                     /locus_tag="PK015_01250"
     CDS             complement(22300..23067)
                     /locus_tag="PK015_01250"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF010587.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HOL33133.1"
                     /translation="MRNLDLYKEKERIIEFIRGYLSNAERDRLVVGLSGGVDSAVSTA
                     LAVEAIGAQNVTALIMPYETSNAESVADARDLAKQLGINYQIIDITAITKAYFETYAP
                     EADHMRIGNWLARIRMCVLYDFSAKLKALVLGTSNRTELMIGYFTQFGDSACAFEPIG
                     HLYKTEVWKLAKILNIPEKIINKTPTADLWPGQSDEQEIGISYPVLDEIVYELTELHL
                     SISATENLSYPVELYQKVERMIQYSAFKRRLPPILES"
     gene            complement(23391..24956)
                     /locus_tag="PK015_01255"
     CDS             complement(23391..24956)
                     /locus_tag="PK015_01255"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HOL33134.1"
                     /translation="MRCLCSVLLILFSCFLSAATVISGGNVSGVWSVAGSPYLINGNI
                     IIPEGESLTLNPGVELQFQGYYTLKIQGRLQAIGTFSDSIKFSTASNSQKWRGILMQD
                     IASSNDSTIFRYCSLSRGDARQDSQDKNGGIIRVLNSSRLLIENCCFSYATADSGGAL
                     YAKNSSFKVKNCFFYNCSATYDGGAIFTSLPDLNNQTSLYIEHCTFKSNSAGKMGGGI
                     TLCGGSHYIICSKFSNNYAQNGGGGIALKNNCHYLCLNNLIVNNGTSHQGGGIFASSY
                     ASGNSIINNTICNNSATINYPNGGPGGLYIRGYADCYNNIIWGNSSSQGQIGIRTDGP
                     SQFFDYNDIENGLAAFNISWENEPWQDSQYGNHNITGNPQFIQPTIDNSLNLDGLAAD
                     WRLHQNSPCRNSGNPFTDISLYLFDLTGNPRIMENYIDIGAYEYRNMSIPQVPQNVRI
                     IKNSDLLYLSWDPVNQALDGAPIYNISYNIYYSLNPGGIWEYLGSSDNPFFILDSENN
                     SQAFFKVCALANP"
     gene            complement(25051..25809)
                     /gene="recO"
                     /locus_tag="PK015_01260"
     CDS             complement(25051..25809)
                     /gene="recO"
                     /locus_tag="PK015_01260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOL33135.1"
                     /translation="MKGKIKDQGVLFKTTQFRESSLILRVFLKYRGLVSILAKGIRKK
                     PENNLLNSLYTYDFTLYEPQESGLYLLSEFTLLKEFNLFDNIPAWTAAECAAELYTQI
                     IIPSEENPAYYDLLQSYLDYLKIRSDNSILLWWRFLLRIFRMLGIAFPLDLCAVCNKK
                     KLQIVAWEKGTGRLICAECLKEANYPEYYEVLTSSASQILHLLPVIGDYLDKIHPDSQ
                     TVEQLNHLFSDHYQNHFRKPLKLRSLEVLSQFFQ"
     gene            complement(25806..27236)
                     /locus_tag="PK015_01265"
     CDS             complement(25806..27236)
                     /locus_tag="PK015_01265"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HOL33136.1"
                     /translation="MDYIILFDWTKEFSVEKKIVECVFYNDKALRIHFKDNTDLYLVI
                     SNYDAYPFYTSKPFSIGDETPIWDQLIHSVLTKVSLDEYDRIMRFIFTQIDIFQQKKT
                     YVLIAEFIPPKPNIILAEQNQELIIVDALRKYSYADNPQRQILPKIPYQPPKTAFKPY
                     HRDISFPLILQTLKTGETIQCDTVNEYLKNHFIYVLSVKEELEHRKAIVDYWERELKK
                     TQQKLHKQRMELEQAEKSDYWRICAEIIKVNLSNIQRGQNVLKAINYFDPELSTIEIE
                     LLPDKTPQENMQYYLKKYKKAKRGKEVIEHNIAETEKKIKQLENIVQKAKRGELDELP
                     QKEKLPQLSRKLEMAEKLLRLRIDDRFEIVIGRKASENDFITTQLARPHDWWFHSRLY
                     HGSHILLRCLQKTNPPEELIQICCSLAAWYSKARFSQNVPVDYTQVRYVRKPHKSKPG
                     FVTYTNHKTAFVQPMDLRTAKKELNL"
     gene            27319..>28190
                     /locus_tag="PK015_01270"
     CDS             27319..>28190
                     /locus_tag="PK015_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33137.1"
                     /translation="MNKTTWIIIVVVLAILAIIFGVMWNNASVKAKKLAQAQEELQKS
                     ILEKEEPVTAEELQNSIDALGKELGLIGAGTEFPVGTPEELKARNLNTLNTARTKIAE
                     YKKQIADLESKIAANKKTMASMQSTVDRLKKSLAEKERIVAELEGRVSALNGTLDSER
                     KAAQEEIAKRENMLKEKESELNKVNIDNNTLYYAIGTRKQLLDSGIITRKGGILGIGK
                     VSTLKDVNLTKFSQFNLLETQEITFPVTKKGYSVLSNHVAASYEVRQEGDQYVLTITD
                     PELFRKQKVLVIEL"
     assembly_gap    28191..28290
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(28362..29387)
                     /locus_tag="PK015_01275"
     CDS             complement(28362..29387)
                     /locus_tag="PK015_01275"
                     /inference="COORDINATES: protein motif:HMM:NF018816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOL33138.1"
                     /translation="MENLAVQLAFVFLLSVLLLLSKGGKRWLAGLRLMLPFLVSLIIV
                     YSLFGLFRLHTPEGEPGSFQFWLEFGSIRILLFVNSVLAFQLFFSIVGFNDLLHLPLK
                     IGTMKYVILGKVLSNTAINSYQEITFHQSLIPSEQYEKLPFSHRFKARLSSVLALMLS
                     LITEASHKGQYIDNRIIHCHQVKKYNPNQWYLILGFLVMVTVATMIIPIPVPGGGFFN
                     FGDVMIVFIGLYAGKKAGAIAGGIGSAIADLLLFPLFAPITLIVKGLEGFICGLAHQK
                     SGFWQIIFPLIGAIVIIVGYFMGEWFLPQLGKAVALADLPVNIVQALVGFVGGRALFE
                     AAKYLNL"
     gene            complement(29446..29961)
                     /locus_tag="PK015_01280"
     CDS             complement(29446..29961)
                     /locus_tag="PK015_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33139.1"
                     /translation="MIRLESGTYPVWDDFSLELTSDLTFSSVALYYLHGANGSGKSSF
                     IERLLIPSLLNQKDIFLLYFEQQMHFQIQAVKAYASIMPPRKEIHNEMDTVDYLLNNL
                     LFNYSQEPRPCFIVMDESPYEMKIYDFIKQYIPDYCLIYSAHSELLPATKTLEFIPVS
                     PSFSKIYVPFN"
     gene            complement(29948..30316)
                     /locus_tag="PK015_01285"
     CDS             complement(29948..30316)
                     /locus_tag="PK015_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33140.1"
                     /translation="MLQKYFNEYLICNARSPLISEGLLREELLLYNISTEKWKELTQE
                     FGDITGKHLGPEDEIGTLSGGQKVLLMCLLALYSPAKKILFIDLWRSLDERNRQKIED
                     LLEVYSREKEIRQEEIGDQT"
     gene            complement(30324..30413)
                     /locus_tag="PK015_01290"
     tRNA            complement(30324..30413)
                     /locus_tag="PK015_01290"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30377..30379),aa:Ser,seq:cga)
     gene            complement(30414..30878)
                     /gene="tadA"
                     /locus_tag="PK015_01295"
     CDS             complement(30414..30878)
                     /gene="tadA"
                     /locus_tag="PK015_01295"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HOL33141.1"
                     /translation="MKDEDNIHINWMKVALEEARQALKEDEIPVGAVLIKDNKLVLKE
                     HNRTRQYCNPLAHAEKMIIDRILSSEIKYLYDYTLYVVLEPCVMCAGMMILSRLGTLV
                     YGAYDPKAGAVGSVYNVLADKSFNHHPKVIRGILEEDCSALLKDFFQNKRNI"
     gene            complement(30883..32676)
                     /locus_tag="PK015_01300"
     CDS             complement(30883..32676)
                     /locus_tag="PK015_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33142.1"
                     /translation="MKLNLSLLVVIFSLFISLGAQNQNLDLYFFTSQTVQSYENNLYY
                     QLNPSPSWLMFINALDIQEERMNFSQHNRHSVLNLNLSSGENLLRHSILSGYEYFYDS
                     SDLDDNLQPYNSQTGMLGYSLDLVPDDSLLISVGAAGYLRSEKDRYVLGNKLNSDGYL
                     ISGKVSGGAEFWQLTTGFGADIERKKLDWEYYQIGSLSAYLNHLSEQFSFNNNLSYNQ
                     RTDDLFVLLPQAENGERGYYDLYDRQKRQSFQYIGYLEYVPADEAKITFQDVFTKRNT
                     NLDINTVRNNKETGNQASLLLQLLPWNQVGWTTIFNHSYLTKDYNLSQNSRQNENRFL
                     STVLNWEYIQGDTLSAGISVDLQRASFPKDQHKWDNDLRNIRCSLINVHYWKDRLKLS
                     NRFYWNLTDDVYVNSILSGNNKQTTSYIYNPECAILIGDCLLFDQNYVIRADYTDYVF
                     SPSEKGLYRQLKGDYKLVYDNYPFIARSNDQRWLLIPYRHHHQNAFQTSINFGFEQNE
                     YADYDRSVYVINFRNNRYTLGLTLKHDIYDFYYYLQPQYSWGTWQEYDFQAGLVWKLP
                     GSSLLEFTINPVGKDLQNLDWRTSISINAAF"
     gene            complement(32639..33412)
                     /gene="rsmA"
                     /locus_tag="PK015_01305"
     CDS             complement(32639..33412)
                     /gene="rsmA"
                     /locus_tag="PK015_01305"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOL33143.1"
                     /translation="MRPTKALGQHFLKEPAIAEEMVKLAEISSEDRVWEIGPGKGILT
                     QALLAKGCKLTAFELDDRLEPLLKNEFGDKIELKMVDVLQIDWELELKKESTPVKLVA
                     NIPYNITSPLLEKLEEYHSYFSSITLMLQEEVAERICAIPGTKAYGLMTLRLQRIFDS
                     SILIKVPREYFEPIPKVNSAVVSLRPRAKPAEIPDLDKYLTVLETAFAHRRKTLRNNL
                     LHLTDRDHLAQIQEETGIDLNRRGETLSEAEFITLSRYL"
     gene            complement(33478..33654)
                     /locus_tag="PK015_01310"
     CDS             complement(33478..33654)
                     /locus_tag="PK015_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33144.1"
                     /translation="MAKNHRGKGLKNLPNHGRGECPVCHRTGVKLLYEVKVGEQTHKV
                     CKNCKQSAAQKLSA"
     gene            complement(33806..34255)
                     /locus_tag="PK015_01315"
     CDS             complement(33806..34255)
                     /locus_tag="PK015_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOL33145.1"
                     /translation="MSRTSESALQQEILSWIRQNQYVYLATVDKRQPHVRPMVMFVFD
                     DRFFFVTFTGDAKVNQIANNKWVEVCVPLHESGNTGYIRLTGVAHIVKNPALKAEAAE
                     QCFFFDEYFRGYDDPDYTLIEFMPETAEYLRPGERYSQTCNLKRYSG"
     gene            complement(34265..35119)
                     /locus_tag="PK015_01320"
     CDS             complement(34265..35119)
                     /locus_tag="PK015_01320"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOL33146.1"
                     /translation="MKQFPGISAMIMLVLAFSLVLVNCNASKTESSESDALKGQKDLT
                     AKKITAIIETNYGNIELELWPDMAPKTVDNFVKLSKEGFYDGTYFHRVIPDFMIQGGD
                     PNTKDKDRSNDGMGGPGYQFEDECYIIGEEIKGMVNDEDTAMLVWDHLIVPYMQREEN
                     PVKELMDIAQEVVEKQSPEPMYGKTIEWYQTMTGISTPIHKQVLKSPVLYSYICMANA
                     GPNTNGSQFFIVTKKDGTPWLNGRHTVFGKVVNGMDVVHKIENLPRDGNDNPLEGNQA
                     FINKIIIP"
     gene            complement(35116..35856)
                     /locus_tag="PK015_01325"
     CDS             complement(35116..35856)
                     /locus_tag="PK015_01325"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HOL33147.1"
                     /translation="MRICVVSDMHYPYQMLDERDKENAELVLSFLTEARGNYDILVLN
                     GDIFDLWFDWKYTIIKQYFPLLHRLAAIQDEGCQLVMISGNHDFWFNDFFQKYLPIPI
                     YDESYNLIADGKKILFTHGDLHPEYDVRYKLFRRLIRFGGIKKIFSWFHPDFSLAIAA
                     RLTRSSRMKNFMQPKKTVGLKKYAKYIITKEKYDYVCMGHSHQPEIQEIGNGKYINTG
                     DWTRHHTYVEIIDGMVEIRQYKIKEIGK"
     gene            complement(35858..36529)
                     /locus_tag="PK015_01330"
     CDS             complement(35858..36529)
                     /locus_tag="PK015_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HOL33148.1"
                     /translation="MKKYILILIILSLFALLSAVIGNASQVGFARLQYDGGGDWYNDP
                     EVLPNLARYVNSVLNTNFPIEQSVVKASDPKLYDYPFVYMTGHGNIRWSEQEVQNLRN
                     WMLRGGFLYADDDYGMDTAFRREIKKVFPEYELVELDSRFPLFTCFYDFSSGLPKIHE
                     HDGKPPQAFGIFDDNGRLMCLYTYETNISDGWADPYTHNDPEEVRETALKFGCNIIYY
                     LFCRG"
     gene            complement(36529..37905)
                     /locus_tag="PK015_01335"
     CDS             complement(36529..37905)
                     /locus_tag="PK015_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33149.1"
                     /translation="MLTIQSKFDSYPQSMCEYRASKDGRYGWFLCTTHQENNVVLEEI
                     QAEIKLEILNANVLDISKLEVENWMQHFFSELHWKLHGRFLKTDLREKGISLFFAVLF
                     DCDLYFVQFGRVFCALTKGKKLETIGKNWKNYHIQSLNELNLLGWSEGDIKVKTYKCH
                     IPENENFIVLPGNIASQIFNEKTDVQSIIPLIETHSASPGAMWLILKNTPLLHKPPKR
                     KLTRLEITTLLLLLGTVLAIIYMIFGNRIIDVMLHRTEKEVETTQIKASTVILENLGK
                     VVNAPARSIELQPDWNAELPYEITAPPAFDQQKLYLVSANNLYVYQLKTRSPLWQKAL
                     EDSILSIHPTPFGLELYLSNNKMIAIDEKGNLKWTLTLAANVESSPRLDLIVITPKKD
                     RRIDRSITVVPLERGISVLDSQQGLVMSELQLKEKLLYLSDYDDYNNCFYAIVGNKLV
                     CIKLNVVN"
     gene            complement(37907..38989)
                     /locus_tag="PK015_01340"
     CDS             complement(37907..38989)
                     /locus_tag="PK015_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOL33150.1"
                     /translation="MIKLMFQMIGCWLMKHLGFPRVLPINYTVSLLYTCNSRCSTCNI
                     WKKKAKNFTVEEYKKTFQKIGKAPYWITFSGGEPFLRSDIVEVVTTIYNISRPHIMNI
                     PTNGILTNVIVDKVAAIAKACPKAQLVVNISIDGLEEQHDAIRNVPGNYQKAVATFHK
                     LKALNLPNLQVGIHTVISKFNVDNFPAIASGLMQLNPDSYITEIAEERVELDTIGSDI
                     TPSLVAYKSAIDYLIHRIKNEKYKGMAKITMAFRIEYYNLVKKILRDKKQVIPCYSGV
                     ASAQISPDGEIWTCCIKAKSLGNLRKHNYNFREIWFSPEAELERRSIHKKECYCPLAN
                     AAYTNMLMNIPTLFRVFWRSFIKWWS"
     gene            complement(39006..39905)
                     /locus_tag="PK015_01345"
     CDS             complement(39006..39905)
                     /locus_tag="PK015_01345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOL33151.1"
                     /translation="MDSNSKIKCSLGVFAYNEAENIIPLLEALVNQKLEKVEIEEIIV
                     VSSGSTDGTDELVTQFAETHPQVRLIRQAKREGKSSAINLFIKEAKSDILVVISGDVI
                     PAPTTIEKLVSTFEDPTIGATGGRPIPVNDDKSFMGYAVHLLWRLHHRMALISPKLGE
                     MIAFRKVMDAIPSDSAVDEASIEAIVQSQGYKLKYIPDAIIKNKGPLNLKDFIKQRRR
                     IQNGHLWLKKKQNYEVSSQDMGTLVKVVLKEIREYPSTAFKVIAVMALEAFCRLLGSF
                     DFYVKKKNPFAWDIARSTKNLHH"
     gene            complement(39911..40381)
                     /gene="nusB"
                     /locus_tag="PK015_01350"
     CDS             complement(39911..40381)
                     /gene="nusB"
                     /locus_tag="PK015_01350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOL33152.1"
                     /translation="MGQRRKARELAAQTLYALSFREFNPEYIEHEMLNVYPEILEELA
                     EVDGLANYSEVFAFANDLVMNYIIHQKDIEKEIEKHSENWTISRMSTMDRSILYIAVY
                     ELMFTDTPPPVIINEALEIAKKFCDRATGKFLNGVLDAVNKDLQEREKIEGSKL"
     gene            complement(40365..40844)
                     /gene="ribE"
                     /locus_tag="PK015_01355"
     CDS             complement(40365..40844)
                     /gene="ribE"
                     /locus_tag="PK015_01355"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOL33153.1"
                     /translation="MKVIQGNLISREYRFALVVSRFNEIVSQKLVDGAIDFLLRHNVK
                     EEDIDIIWVPGAFEIPQVAKVAVQRRDIDAVICLGAVIRGSTPHFEYIASEVSKGIAQ
                     LALKQDKPVIFGVLTTDTLEQALERAGAKSGNKGYYAASAALEMLNLLSELKIGTET"
     gene            41284..41757
                     /locus_tag="PK015_01360"
     CDS             41284..41757
                     /locus_tag="PK015_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOL33154.1"
                     /translation="MNRLIEPELVKIVNSFDSKNDCLAYMVGLLAESGCLNKPDRFLA
                     AVKGREEIMSTGIGRGVAIPHARDLTVTALKMAVCVIHEPLDFMSVDNLPVQLVFMIA
                     VPQDLNKEYMKILRVLAEYLRDDNNRTTLINARDEKELYNEVQHIEETMVNALTT"
     gene            41708..42670
                     /locus_tag="PK015_01365"
     CDS             41708..42670
                     /locus_tag="PK015_01365"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOL33155.1"
                     /translation="MKCNTLKKLWLMLLLLSLSFIPVFAVNEEAGTTGFANLKNVYSA
                     RAIALGQSLTGQVRNPDGLYFNPAAILNIEGKELGTTYTNSFIDTQGGQIQYLLPKNK
                     FTAWGFAIKYMNMGSMERTEVDNNGDLISTGETFGAYDIIGSVSLAKYITDAVDLGGT
                     LKVIYDQIDDKSASAVMVDLGMIHHPANPNVKVGISLRNIGFQTSYYSATDYKEKLPF
                     TFAGGITYQFIPTLLGCLEIDKVTGENILAKLGLEYELNPALALRAGFRSNASEGYNG
                     GNLAFLSGFSLGAGWNWKNCRLDYGVTSYGDLGLINQLSLSYEF"
     gene            42702..44801
                     /gene="glyS"
                     /locus_tag="PK015_01370"
     CDS             42702..44801
                     /gene="glyS"
                     /locus_tag="PK015_01370"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HOL33156.1"
                     /translation="MSDFLLELGFEEIPPSQLQPVVEYIQNSFLNLMKSTALSYSALK
                     VSSTPRRFFLLASSVQEKQEDLQVKKIGPAKKVAFDEKGNLTAAALGFLKKNHSKPED
                     LYIETTDKGEFIALNYVQMGKATPDILKDWIYELIPHLPFTKTMIWNESRMALARPLR
                     WLCILWNEEIIPLEIAGVKSGNITFGNRYLGLNRPVKIDSPSVYLSTLQENAVLAERA
                     YRKEKIIEQLDGLPLENGLQIIPDKQLIETVTDLVEYPTAVSASFQEKYLFLPDKIIT
                     STISQNQKCFSVQTKDGKLSNKFIFISNGNPDFSEVIRKGNEKVVDARLADALWYFQE
                     DTKQPLEAFVPQLKEVIFQSRLGTMADKTQRIEKITEYICLLLHLDTQQKELALRTAL
                     LCKADLVTNMLGEKEFTKLQGYIGKQYALAGGEPYEVAEGIYEHYMPRGPNDSLPQTL
                     SGAIVAVADKMDSVCGIIGIGLIPTGSADPFAIRRAANGVVQIIADREWHFNLFSLIH
                     YTLELLKNYTQLTATAEQDVQDFFHQRVEWLLKQLKLDYDVIDSLEHLALNDIPDLIQ
                     RAKALQAYRAEEDFIRLVIGFKRVSNIINGEKDFLPLKEDLFQQEEEKILYSELLKLR
                     DKIDLCLRESDYKGAIRLLIDYGVFIDNFFDAVLVNCDEPELRANRYALLKEIKNEFL
                     RIADISRLVVETNKNEN"
     gene            44864..46066
                     /locus_tag="PK015_01375"
     CDS             44864..46066
                     /locus_tag="PK015_01375"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOL33157.1"
                     /translation="MSLKISNRLKLVQPSPTLTISNKANEMIAQGIDVVNFGVGEPDF
                     NTPEYIKRAAIDAIEANFTRYTANAGIPELRRAICNKLLRDNHLKYEPKQILVSPGAK
                     AAILNVLIAVCDVQDQVLMPAPYWVSYPYQAILANAEPVIIPTSEENNYKLTALALEE
                     AIKTHPCSKVLLLNSPNNPTGAVYTKKELEEIAEICLKYKILVISDEIYERLVYDDTE
                     HISIASISPEMQEQTIVINGVSKAYAMTGWRLGYAAGPSNIISAAGRVQEHATSCVNS
                     ITQKACVVALTEEDDSIENMRKEFEKRRNFLFTELNKLPHINCFKPQGAFYIMPNIGW
                     YLEHNKQYINGCDQFCEILLEKHYVALVSGTSFGLDTNVRFSYANSMENLEKGVKRFG
                     AFLEELKP"
     gene            46088..46312
                     /locus_tag="PK015_01380"
     CDS             46088..46312
                     /locus_tag="PK015_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33158.1"
                     /translation="MLKNEEKPHFDWRTPRKKNYDWKRLIIMVVLLVVILIAIDRLNK
                     ISKVTEQPAAEFIESDTLNSGINPLPHPSK"
     gene            46309..47199
                     /locus_tag="PK015_01385"
     CDS             46309..47199
                     /locus_tag="PK015_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOL33159.1"
                     /translation="MSLVVVGSIALDSIETPKGKIENSLGGSAIYASLAASYFGPAYV
                     VGVVGEDFPEEAIRLLKEHNINLEGLETKMGKTFRWKGVYNNWNQAETLNTELNVFAD
                     FSPSLPDSYRKCNSLLLANIHPELQGKVLKQMKSYRWVACDTMNYWINLCPEELTKVI
                     HKVDILFINEDEIRQYTGIDNIFKAARMILKSKVQVVVVKRGEYGSVAILPDDLFFSP
                     AYPLEEIFDPTGAGDSFAGAFMSYLASQSSLDKNTIHSAIRYGTVLAAKCVSDFGVRG
                     LIHQDFSDLQKMVDQLESWT"
     gene            47206..48240
                     /gene="purM"
                     /locus_tag="PK015_01390"
     CDS             47206..48240
                     /gene="purM"
                     /locus_tag="PK015_01390"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOL33160.1"
                     /translation="MNNNKLDYRSSGVDIKAGEKAVEAIREMVKGTYNPNVLSELGSF
                     GGLYRFDSSSYTEPILVSSTDGVGTKIRIAIEAQKWDTVGQDLVNHCVNDILVQGAEP
                     LFFLDYIGLGKMNPDKVRIIISGMVKACKENGCALIGGEMAEMPGFYQEEDFDLVGTI
                     VGVVDRPRLLPRNIQKNDLIIGIPSSGLHTNGYSLVRKIIFEKLKLNIDSYIPELNST
                     VEEALLSIHKSYLPLLRPFLSIPYLHGLAHITGGGIPGNLKRIIPDGLTAYLNYESKN
                     MPPLFHWLQDSGNLSWEDMLETFNLGIGMIAVVEDKYSDEFITATASKVLGYIDESAD
                     KKEKVIVSYK"
     gene            48456..51506
                     /locus_tag="PK015_01395"
     CDS             48456..51506
                     /locus_tag="PK015_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOL33161.1"
                     /translation="MRKPALILLILLIGCISLLNAATTGRLAVRVRDNNNKPLEFVNI
                     VVMRGNQRITGGQTDSKGVAIIINIPPGVYTVKLSLIGYDPITYTDVRIEVDETTNIN
                     AIMKKSGVKLAPVVVTANPDVVGKSQIGSVRKIEMERMSEVAAADLTDIVSLQAGVSN
                     IGGELHIRGGRSNEINFTVDGMSVSDPVDGGSALQVDTDAIADMKVMTGGFPAEYGNA
                     QSGVINIVTKDGDPFYSGKVEYNTDHLIGEGRNSDVTKFALGGPVWPLGGQDLRERFT
                     FYLNGGGEWLDGRLKDYYIADPMKDFTFNGIPILEYEYPPSNPYADRKDFLGIDLGNR
                     NYNSYNINLKSKYVFNPAQKVTFAVRGDRSLNYPFSYAWRYALDHFAYDETIQRQYIA
                     TYDHVFNSAMNLSVKASYYQKDSNSGPRGIQRNNYIYMYNNLDPNNPGPDYVDNVLMG
                     IEGWDTVDHDNDGVYSPDYNGDGISDADYLPAAFWAYRIAGLEDPRSIPGFLPPGTIY
                     QYFVDDVTTILSGRADFEWQLNETHLAKTGLELIKHNIKKNQLQNFLNIYQDRYQASL
                     KRVYDMANLQWTPPSDAPGDTTYYGIVPELYAIYVSEDNVLIPIYNPNDYYKAAREAS
                     GKRDGYQAFPWQAAYYIQDKMEWEGMIVNAGLRFDLWYLGSKYKVLQDDGSYRTVEFD
                     PDERLQVMVSPRLGVSHPITDRDVMRFAYNYQNQLPQMQYIFTSKTPSDAYISDQIIT
                     VGNTKLEPQITVTYEVGLSHQLSDDYVVDMTAYYKNIYNYVSTIKEYDPNEPQVYWYK
                     YISEDYGSARGIDIQLEKLMSNFTNWSIAYSLAWAQGNNSTTVIQDEATNLREFPLDW
                     DIRHNIGINFTFRIGRGEEFFIPFTNYILPLDDFTANFNWTFASGAPYTPQSLIGDKL
                     LDTNSARKDPTHQLNMRLTKGFMLSNKMNIRVFLDVENLLKTKNVLTVYPKTGLWYDD
                     GADLADSNTGYVYPEVKFVHDLYTRNPGFVNDFRGVTLGISFNF"
     gene            51534..52352
                     /locus_tag="PK015_01400"
     CDS             51534..52352
                     /locus_tag="PK015_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOL33162.1"
                     /translation="MRNRISKILPLLLLMSVLLISLAYAQDNATATAPDTAVTTTTTT
                     EQTSPTETEAVQKSSGLRSVAEFLFGRALVQEFLNGGWAMWPILAVFIYGIAYSIWKF
                     ISLMYAKINLNDFLNQIVPLIKEKKIKEAIETAKKTRGPVAAVVFAGLEKAEQGIEAV
                     EKNMENAAMIEMSYLEKGFVEMTSTIAICPMLGFLGTVDGMIIAFDAISKARAVDATI
                     VADGIKVALITTKWGLISAIPVQLLYNIFTTMVDGIVVDMQRASEKVTEALIEA"
     gene            52365..52838
                     /locus_tag="PK015_01405"
     CDS             52365..52838
                     /locus_tag="PK015_01405"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOL33163.1"
                     /translation="MNVTRKIRPRASIPTTSTGDISFLLIVFFMSTTKFDVKEGVKVQ
                     LNKAVPEEQVRTTEIKLTEAEMTRLEILPDGRIKVNNEEPRVFTDEELVNLIQSKIEM
                     RERLNQQSSDPEVRKTKMLFLVKTDSEAKYSDMVRVVDHLVSFRDRAMISISTQL"
     gene            52848..53273
                     /locus_tag="PK015_01410"
     CDS             52848..53273
                     /locus_tag="PK015_01410"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOL33164.1"
                     /translation="MAKIQKTKRPTASIPTTSTGDISFLLLLFFMVSTVFVQERVFWV
                     PRERLPFATENIERIARNETATIYITLKGQILIDDFPYSVEDESIVETMRTKAREIPT
                     LQVCFRADKDCRYQSIRDVMIRLQDADTRNVVFEVQRKL"
     gene            53286..53939
                     /locus_tag="PK015_01415"
     CDS             53286..53939
                     /locus_tag="PK015_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOL33165.1"
                     /translation="MENNKIFDWKDYCDAQFSKAVALAIAIMLLIYLVAPKTETQTKK
                     EIISQVEIAEAPPDTRQKEETQTETEIDIDIPVISEELATEDTPELAEKRALALQQFG
                     NLQSTTSSTIQQSGEERPFDFVPWDDPPVPIGSIRPVYPEFARRAGIQGTVILEVDVY
                     KDGTVGNIRVVRSVQPGPGGLDEAAIEAVRKVRFQPGKSSGNPVDTTVIVPIEFKLN"
     gene            53960..57025
                     /locus_tag="PK015_01420"
     CDS             53960..57025
                     /locus_tag="PK015_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33166.1"
                     /translation="MKFNKFIFCLLLALLFIGIAQAAMVDKPSGAKKLDIDHYHNVGN
                     MWLRVSNYGFFGSGDDAVPQYPSLEYPGGQGIDYLYQGALWFGAKKYRRDSAGRKLYW
                     RALNPSADSSQTVPYGSPDWQPWMKPVMDTLVSVGFDGDWDIYEFLPAYNPLVSANTN
                     PEVTAMFGIYNGLDRMITSSTRTQKRGVDDDGDGCIDEDFVGYTFPLRRADELPIQFQ
                     PFGGQFIADTNNYNIINTGNNMEIWFPLGFMDLSDTSFRSYCFSMPYDDDNDGQIDED
                     GAPVSEQDFIGYYYDYCPFGTPGDRDYGGSRGSSKHFPLNVRVRQMSYQWSYEYIKNL
                     VYVEFDITNMNTVYMDTLFDCAMGIYMDCDCGPQVWGREKAEDDVSGYVKGSGYEFAY
                     TRDADGDGGLTTGYVGARVCTPDPEQLQFHCWYWKVGNGPKDTDPLNLNYAPKKTANE
                     KYWLLTGRNPDETKFDPLRPEQIDQMEFEETVPKDTRFLFAFYGDQNGVTAPTENSWN
                     LAPGRTMKIVVAVFPGDNLEDLKSTATFAKMIYGQAQTLNTVVLPDTFPHYSPPEPPD
                     IPKLFAEMTDDGSRIELYWDNRSEFSYDVMTVSSSVIGWQDPNSPFLIPGIDSDPNSV
                     DWSNFPDEFKPNPNNYNMNARVNPFTGYRLRHDFQGYTTWGRSGSGSQEDWVMQERWD
                     KIDTPQDLLDYNVNIGTEYYINYGGYLGIDKGLPNKNQWTGDITKFYRYDETYQFVPY
                     ENGDDFYGWPIYNPEVEWSLELQQQAAQIEVDYADLPPSIVKNLKARLFKHPEIRDDI
                     FDAIYDPKMIPLPGFGGQVYIPTNPNDQSQWEKLTELKKQRLARRYYHSAILYPPKGI
                     EYYIAVTTFDRGIPLNHISSLESGRDKDANMKVFFPGTVAKSDMNNIYVVPNPYIGKS
                     KFDGRREKDEKGDKSRRIWFVNLPERCKIKIYTLAGDLVDEIDHDGSYQADILTVSKA
                     TTHGLTASGMHDWDLLSKHNQIIAPGVYLYSVENKADNKIKVGKFVIIK"
     gene            57035..58108
                     /locus_tag="PK015_01425"
     CDS             57035..58108
                     /locus_tag="PK015_01425"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOL33167.1"
                     /translation="MKKNLIFLIALSLILIPTLISAKPFGKVGTVALQFLKLGVDARA
                     IGMGEAYTAVTDDISSVYWNPAGLAPAYQNQAFVSHTNWVADIMHEFAACSFTNGVST
                     VAAYGSVLHMDDMDITDEETFGPTGEKFTNSSMAFGVDYAQQFTNKFSAGVGVKYLRE
                     NLYEFSVNSFSVDVGSMYNTGWRNIKIGMALRNFGPDIHYYVDDDEDGATDEDPFDLF
                     DNDGDGAIDEDGPELDSKIPMCFSLGVSGDIMRTWNEKIPTDERTIYNSLNHWIVSIQ
                     LDNVIDRMETWNIGTEYKYGNLFLRAGYQINYDANGFSAGVGYQIPTNFAIINVDYAY
                     TDMGDLAESFLKSAHRLSIKFKF"
     gene            58137..60311
                     /locus_tag="PK015_01430"
     CDS             58137..60311
                     /locus_tag="PK015_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOL33168.1"
                     /translation="MKKTVVIIFTLCLLMSLAFADDMEIPTIMKPITNFQYVAKTAPQ
                     HRDAPSYTFSKLPTPIITNYYDYMIGSYNGLPLKVIPQSAGGGYFMTYHGRRQAGGTR
                     RVFYTYIDAQGNIQTNNEITNLQNHEGYPTIEVDKLQGKPMYAWHANKDSDSELEVEF
                     ASDAFLDGITGLFNYDIIINNPITITAPNGTTTTDNEFIWPNAQIGPSPIAGKQRIYV
                     VARNSVTHNTANAPSENPYIAYADFEPDDIVNAIPLVWHYTSIPEMNQWNVDVEWRRP
                     FHAITADNAGNLYYAGYHFATESDASTNIDEPDLDVFICPNYGEGTWRRVSSYSHLNM
                     WNPNSSPTDTTGYFDSPVSGLPFGDNGELYFGIASGNSGHINATMDNLGRIHVFGVWS
                     LQNIDGYYYPEMEFVKEFIFDPATEEFSIREVYPQKDPEDNFNTGFCPWDYQPPFGEV
                     DEYVLTSSGYAISVATGWPFPHWDDSAHSDAMEFHYNNCKITSANEQGMMAAVWQDSR
                     RARDYNYIGDTSMAAYANTPEIWISISPDNGNSWSEPIIINNVDTPEFAGIKPMWVYP
                     ADKIQYVGTVDGHKIGKLGIMFYNDYTWGANVIDPPYHQNPDGGQVMFMELLITAPVA
                     NDDLTVPNVTYMLHQNYPNPFNPQTTISFDMPKAGFANLSIYNVKGQLVKNLVNDNLD
                     FGRHSVVWNGTDNNGKPVTSGLYLYRLTTNGVTETKKMMLMK"
     gene            complement(60628..61548)
                     /locus_tag="PK015_01435"
     CDS             complement(60628..61548)
                     /locus_tag="PK015_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOL33169.1"
                     /translation="MKKEHWLIISLILIFLAIAALYLFIGNVSKEIVIQVRLPRMILT
                     IFTGMTLAGIGSVYQLMLVNPLAEPYILGISSGSAFGAILFGVLGLTLLMPVGGFIGA
                     ALTLIIVWRLAQKKGSFDRSRLLIAGVIVGMFFSAGISLIMYLFQKDTMIILGTLMGN
                     LGHIFTQSEWHFFLFLLVVSIVILGRLYFRSTALDIMSGGDIYAGSVGIDVQKVRKEL
                     FLLTSVLVGITVSYAGIIGFVGLIIPHIVRFFIPSGQRKIFLYSLITGGIFLLCCDLI
                     AKNITVIELPVGVITSALGCPFFIWLLLKK"
     gene            complement(61545..62405)
                     /locus_tag="PK015_01440"
     CDS             complement(61545..62405)
                     /locus_tag="PK015_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HOL33170.1"
                     /translation="MMKLRKYDVCVCLMFLLMMILVSCNKQEQVRNGQKIVVLSPEVA
                     EIIASLGATDLLVGRTLECDYPEELLKVPVVGNFGAINKEKVIALKPDLVFTSALEQD
                     AIAQELQKIGIKVQQIYPKKLDDLPVIIKQIGDLIGKEKEAQSLADSVATAIEEVRSR
                     AANIHHPRVYVEIYRNPLMSVSDESFVGDVIESAGGDNIFPRLERDYARVDPEDVILA
                     QPEIIICYSQDTLEGICSRKGWSNIPAVKNQRIYFEKDIDPDLIQQATPRTVQGLWLL
                     YEIFSEQTIK"
     gene            62861..66220
                     /locus_tag="PK015_01445"
     CDS             62861..66220
                     /locus_tag="PK015_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOL33171.1"
                     /translation="MNNFITNSKESKTLSARIRELIRASEELKFLVGFFYFSGISELI
                     DSLKENPDCILKILVGLKIDVQNYGIIEYGDSISKSHSEIPEEYLQSVVKALNTDEFD
                     NEIFYEQVRFILRLIKDNRIIIRKTLYPNHSKLYIFNLKEEQVTRNKLFITGSSNLTR
                     AGLKSRDEFNVEISDYGVEEAEQYYDDLWKRAVSITEESDIKDKLIINVEEKTGLKSI
                     HPFDAYMFVVKTWLDSIYKSQEEDSLEEVLLKNNYKPYKYQIDAVKQAQYIIQENGGV
                     ILADVVGLGKSIIACLIAYQLKGRGIIICPPGLIGDDAASSGWKKYKEDFGLYGWEIF
                     SGGDLDTVLKFIENKDNIETVIIDEAHRYRNSSTESYDMLKTICRGRKVILLTATPFN
                     NTPADILSLLSLFIVPKKSNITITSNLSDLFHFYNNLFKELAFIQKNYKSTDPKNSGD
                     AIKKYRALFGSEEIDIQKVTARAKFLSQQIKEVLEPVTIRRNRLDLINDPDYMDEVND
                     LPRVEDPKECYFQLTKEQSEFYDRVIGSYFTNNPDITDKFKGPIYKPYKYEGKDKEKL
                     TLQEQIDQLSQDNLYDILRRQMVKRFESSFGAFQKSIENIKKSYETIRTFIEKSGGSY
                     FLNRSLINKVNYDDPNSIDEILSKYEDSIVDKDDKKYKVYNVNEFTLKDEFFNDIESD
                     INLFSRILAEIDALRLVDNDPKRQSLIEEVENILQTPPKKGEPAPKVVIFSEYADTVN
                     YLAPTLKSKFAEKVLVVTGSLSNTQLEKINSNFDASYREDRQENDYDILLSTDKLSEG
                     FNLNRASTVINYDIPWNPVRVIQRVGRINRISKKVFDTLYIINFFPTEQGSDIVRSRL
                     IAASKMYMIHETLGEDARIFDPEEEPSPAKLFAKIQTNPDKLEPESLYTKLKNKMREW
                     EQLYPDRVKALEDMPIRLKTAKPYNSDSLNVFIRKGRLFCVSTDAINNNGNYQTLPME
                     KAIERIECSPETLHLPLSKDFWNNYTILKEKAGDFFYNQKGRSNVNEAFNVISDLAED
                     SNPKLLPWKSFLFMLKEDIANYGTLSDYTLRNISDWIKIGKDKDYDNIISELEQLSQK
                     LGKNYLDKVKKQLKYYDQEVIVAIENQYVKK"
     gene            66207..69728
                     /locus_tag="PK015_01450"
     CDS             66207..69728
                     /locus_tag="PK015_01450"
                     /inference="COORDINATES: protein motif:HMM:NF019291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HOL33172.1"
                     /translation="MSKNESIVTPDNFSQEKWIDFFYSKSKYFREDNKLNKDYTDSNF
                     IEINKIGKIEFPQSVPSDLGVYALKVANPLTERSSRKKQYDIARNIIINERVESGIFI
                     FYEENKSFRLSFIYPIYQGRQRKYSNYKRNSFFVSKDEPNKTYILQMENYDMHSINEI
                     KDMFSIEKVSNLFYNEFQKEFERLKNEVVQPKFEEVIDENLRNDFALLFVLRIIFLGF
                     VQKKGWLGNDAKFIRNYIDCYDPCLHTNGIYQNLLEPLFFQALNSPHSKKNKFTFPNI
                     PEPFNNALVNAPFLNGGLFHKITDFDTIALYITNEGIRNFVDFLFSYNFTLEENTLYD
                     EELELNPEFLGIIFEKLINKQYGAIYTPQLEVDLMCRLSLVKFLQQNCSSDISYENLY
                     KLFFREFGGEEEQTLGVFTYEQGREILHKLESITVCDPAVGSGAFAVGMLNVLYDTEE
                     EIYKNILNEDINTTPFERKKRIIFQSLYGVEVQKWAVWITQLRLWISLFLEAEDELKI
                     SDEPLLPSFDFKIRQGDSLIQILGDSLFPVSGEGIIGPDIKKNIDKIQKLKTEHYYNK
                     SALTLNEIERLQRKLYINMLEKKKNTLVKKLYRLQIDTTQIQADFFSDEEDSVLELAK
                     LNFEKSIKELELEIKKLDFSIDKIKNNDIPFIWRIDFPEIFLSKEGFDIVIGNPPYVA
                     QEEITDPQQIIDNRTYKALLKKMAAQDFPDRISENSISGKSDLYTFFYIRGLRLLNKK
                     GILTFICSNSWMDVEFGAWLQKFLLENCQVYLIIDNLNKRVFRESAINTIISVISAPQ
                     PRVKNDDLIRFVAFKIPYESSIYTDNFLSIEDARKRLDIDDLRVNVLTRMQLMEEGWE
                     NSEENRYRGSKWGSIYIKAPDIYFTIMEKAKNKLVKLGDIAELKRGYTTGANEFFYFA
                     INDPRIKLLGEDNFKDIIRSPRELKGIDIDKNWLNYKILYINPSIDILSNPYLFQYIK
                     EGEKRKYHLRPTIKNRIPWYSLPPQELSDIVQGQIINDRFLFCLNQKYYADCVLNLLY
                     LNEEYTNLLYETLLSLNASITILMSELNGRTALGEGALKTQVFELRNLKITDPKLIAK
                     FPDSQNKIKNMMKREIYSVCNEFQLNSYQEIKDKLPDPLPDRKAIDDIVFSALELTPN
                     ERKAVYMSLLELTANRLNRANLNQDRE"
     gene            69752..70330
                     /locus_tag="PK015_01455"
     CDS             69752..70330
                     /locus_tag="PK015_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33173.1"
                     /translation="METYELLQEMERLFLVFIRARSLTTYVSEDIIDYVQGKNNTHII
                     LDYDTLYNVYGYTTCLFIEPKNFSTPEKYNEYKSMGSWINASFFIHLNVLIEKYIKEL
                     PIENKKSSDKITDNDYYKILRLLRNKVAHNPFNNIKITTNNKDNCQCFEQLINTYFQQ
                     CIKENNELDISIDKFVIPLFWKIYKLIKTEIS"
BASE COUNT        22445 a        13023 c        12453 g        22415 t
ORIGIN      
        1 atgtccagct tatgtccgaa agtggacata agctcccctt cagctcccta aaatttccct
       61 atcaccatat cataaaaaac caagataaat atctcttacc gcaatgggat ttctctcaca
      121 gataacacag attacacaga taaaagttta tttgtattag gatgaggatt aaaataggct
      181 ctcacagatt acacagattg cacagataaa agtttatttg tattaagaaa ggaattataa
      241 tagaagcacg cagattacgc agatagcgca gattttatta tttatttaaa aaatgtgata
      301 actgcataag atggatagag ttatgaattg aaaaatagct cccacagatt acacagatag
      361 cacagataaa agtttattta tattaagata aggattataa tagaagcacg cagattttat
      421 tatttattta aaaaaatgtg ataactacat aagatgaata cggttctgga ttgaaaaaat
      481 gctctcacag ataacatcct ccatctatat ttctaccatt ttatttgtcc agacatccga
      541 gcaaataatc ttcactgcag aacagtttaa caatatactg aggaaggatt attaccagaa
      601 gttcctgatt attaatttcc tataaatgag ctctaattta tattaattat aacacattct
      661 ggatataatt gtcattcaaa aatttatttt aatagaaaat cctcagataa ttggtcatct
      721 ggaatctttt gttccagggg cagatataga tttacacgta tctctaattc ttttaaatat
      781 ttacagagaa taggaccaac aactccccag ggtaaccaac ctaatccgca tcctaacgca
      841 ggaatagcta atgaggttat tttctctact ttataattat taaccagcca ttgtaatcct
      901 ttttctatgc cttttaaatc ggcaattttt ctccaatctg tcttggtggg aaaaagtaaa
      961 aaccaggtct gtaaatttaa attattcaat ttttctgcat catcagataa gataaagtct
     1021 aatgaacttt ctcttttata aagatatggt ttacccatag ttagaatttt attcttacat
     1081 aaatcctggt atttaacata gacatcagga aattgatatt ttgctcttga ggcaagcccc
     1141 ttacccatta caccttgagt attaacactg atagtaagag tttgcatcct ggaaaagaac
     1201 atatctcctt ccataagatt aagattttcc gtaagaggaa tttgtcttga gggaaggaaa
     1261 aataattctg gttctggtat gactgttatt ctttcatcac atatatctct aactctattc
     1321 atagcatctt gattggcgac ataaatttca cttatatact caggctctac tttttcagga
     1381 actaaacatt ctgccataat ttctcttttt gaaccatctt ctattgccca ccattcttta
     1441 tcggtttttt cacgaatata ctttgatata tccctgtctt ttacaggaaa aaattgtgta
     1501 ttagaactcg ctgcgtttcc atctgttaat ataatatcat ttctatttag taggctattt
     1561 ttaagtccaa tgactacaac ttcatcaatt ttaaaattac tatgaaagag tactctataa
     1621 agcattggat tccggggttg aaaatataaa tttacaaaat cccataattc tctcccatca
     1681 ggaacccttt tgccctttct gagggcaata atatcctcat cgtaaattgg cgtaaaatta
     1741 atttgttctt caattatctt tctatgacat aaaatccctc ttttcaaaat agagagtaaa
     1801 ttctcaatat gggtgatata atacaattct ctaagtttta acatattatt acctatttta
     1861 tgatttaaac ctataataac aaaaaatagt ctattgatgt ttcaatataa agtcctgaac
     1921 gatactaatt caatctctca gctataatat tcgtaaagta atccagatat tttcattaaa
     1981 aaaaagatac tttttctgtc tatctatttt tctattctgg cttgttggtt catatgaatt
     2041 cagttcaaga atatcttctc ttaaataatc aactttattt ctatactgat tccacctcct
     2101 atttataatt ataaaattaa cttacctctt ttaataagat gaaattcaat gtttccatat
     2161 tctttatgta cttgtaataa aaggaattat atctacgatt gatcaacttc agtaattcca
     2221 cttttttcta attccttttt taaatattca agtggtttgt tatttgaatt gtttaataaa
     2281 ctataatagt gttctttgac cacatcatca aaataaggag ctataagttg ctgaagttca
     2341 ggtaagttat agatctctaa gtacgaaact aaattattac ctttatttaa atactcagct
     2401 tcagcaaaaa tccaatcctc caatgcatct attctaagtg cagcaagtaa aggtttatat
     2461 ccatcttttt gtctatcagc taatccctta atatccttat ccgcaatatt tatactatca
     2521 tctgtagtgg ttttaacctc tatagcatat ttaattccag cattatttga agcatctata
     2581 tccacaccct gaacttctcg ttctacaatc ttatcaaaac ctgctctttt acaacttaaa
     2641 gcaagcagtt tctggcaaat tttgccaaat tctattttat attgttcatt aagatttttc
     2701 agcatctcta cggtttcgta tttcataact cctccttatt aattattgga ccggttgtat
     2761 ctgtccaatc ttcaaaatta tgacaggtct tcattttagt gattttttta ctaagcaaat
     2821 cctgcaattc tgcatccata gtagatacta taactttttt acgcttcaaa acttcattta
     2881 aaatatccac taaattcctc ttatgatgag aacccatact ctgagatgga tcatccataa
     2941 ttataaaatc aaaaggcata tcttctgcac cttccagtgc cattcccaag tatattgcta
     3001 atgctaaagc attcatatcg ccttgactaa gaataggaat taaatcttta cccttttcat
     3061 ctttaccttt ttcctctcta cttacatctt taaatttata atcattagac cctattctgc
     3121 ttgattcttc atttatttca aattttattt tcttgattgc tggattatcc gtaattttgc
     3181 aaaaataatc ttctataatt ttactggtcc tttctacttt atctttagct tcccttttgg
     3241 atgcttcgct tatagctaaa ccaatccttt gaatattatt taaagctttc atagcttcat
     3301 ttctttttgc ttttaactct tggaatgtgg gatcttcctc aaaattagtt atatcattta
     3361 tctcttttgt aagttttaaa atctctgtaa ttatttccat aggttctaat tctgattcta
     3421 ttctactcaa attttgttgt ttactatcta ttctctcttt aatagattgt agcttttttg
     3481 ctatctcttc tatttgaata tttagaataa ctaccacatc atctttttcc gttatttctc
     3541 tttttaatgc ttcactcaat gtcttataga attctgataa cttttcttta ctctcattta
     3601 atacttttat taatgaagaa tattcgttta ataaagagtt ttgtttccta aaatctgctt
     3661 tcatactttc taattcttgt gtaagttttt ctatagcttg atcatattgc tctttcattt
     3721 cagtttccag gtgagttaat aaatcttcag tttctttacc acagaccggg cagatatttt
     3781 tatttacttt ctccatttta agatagctaa tagcatactt aatagaagca atttttgaat
     3841 cttctctttt tatttcttcc tctttatctt taattaattt atccaattca tttattttct
     3901 cctgaatagc cttatcatcg ccattttgtt ctataaagat atttaaactt ttttgatttt
     3961 catccacctt ttgtttcagt tccgtaaagc tgttcttcaa atttgataaa tcaatttgta
     4021 ttttattaag ttctatttga gtttgaagat ctggaagttc tgacctcaaa cgagttattt
     4081 ctttctttac tgcatcttta aattttgcaa aatcatcttc tgtttctaat gctggtatct
     4141 ctcctaaggt taaattaacg ctttgagtga aatcagttaa tggtttgaaa atctctgtct
     4201 ctgccttact ttttaagcct tttagattga attgatcctc atctatttca agacttttgg
     4261 cattcatctt ttgtgtgtcc aggtcatttt ttttcaacct taaagtgtct tctaatgact
     4321 tttctacttc tataaataca tcacttattt gcttctcttt tttcggaata ttgttataat
     4381 ctaagttatc aaatatattt ttataatcag aaagtcccat taaacggtct atagcatcat
     4441 ttctatcttt cggttcttgc accaaaaaac ttcttatagc ttcctgcctt tgatacacag
     4501 tagttacaaa atctctatag ctcaatttaa taagagaaga aagttttgaa gttgcatcag
     4561 catcagtaaa agatttatta tcaggaaaag ttacggttaa tgcatccttt ttagcagtta
     4621 tatatttcct caaaactttg tattcagaac cttgttcatc tttcaaagtt aactctacaa
     4681 aagtttcttt tgacggatcc atattccgat ttgggacttc ccaccctatt ctttccctta
     4741 aaccggttgt tccgcctaca gattcatccc caaataaaca ccattctatg gcatttaagg
     4801 tactactttt ccccttatga ttatcaccaa atagaaacac cgcatcatta tcaaaaacca
     4861 gttcctgata attggtatat cctctaaaac cctgcacttt taaagatgat atagtcaaca
     4921 tcatttccct ccttatattt tatttttaac ttcttctatc tttttaatat attcattata
     4981 tgaatgctga aaatactctt caaccatatt cctcactcct acacttcctg ctgctacata
     5041 tttttctacg atcttattcc acaaatcaat ttgctcactc ttataatctt ctttttcttt
     5101 actttctttt aatacttgtt ctaccaattc cttatagctc attttagacc tccttaatgt
     5161 ctttgtcttc cgtctatagt tggaatagaa aaataaacta cggtaaatag cttttcgttg
     5221 aatggattat attctggtct gggaagaaaa gtgtcatcca gaataattgc ctcttgaaat
     5281 gaattgattt ccggattctt ccagccttga cttttgccac tcttactata acctgatatt
     5341 tgataacaat ggaatagttg tttgcctgta gaagatgaat atactatata aggttcaact
     5401 atccgatgat taccattcaa atcaattaat tcgcaaagat gaaggttctt tgccgcttcc
     5461 tggaaatttt ccagtacaga acccatctct tcctcctatt aaatttatta tatatatatt
     5521 ataaacaaaa tttagcgaaa tgccgaaaaa atttttggtt acaatcttta actgtattca
     5581 tcttatgcac ttatcacatt ttctttatta aataaataat taaatctgcg ctatctgcgt
     5641 aatctgcgtg ctcccattcc caatcacctt ccactattaa cggtctttaa tctgtgcaat
     5701 ctgtgttatc tgtgagagcc tattttaaaa aaaaagctcc atcattcaga tggagctttt
     5761 tattgtctat tggtaagtta actattgaaa cgcagtacta caaactgttc atttttgcta
     5821 cggtcctgca cataaagaac aatggtatta cttgattttt ctttcattgt ctttatcgca
     5881 ttatccatag cagcattgta ttcttctggg ctattaactt cggtatttcc aacctttaaa
     5941 atgatataac caggttgaat tccagcacga gctgctggtg tatctggtgc aactgcacta
     6001 actactacac cccgatcagc tttaatgttg ttttgtcttg cataaggact atcaattgaa
     6061 acaacggtta tacctgcgtt ggtatcggct ttagtagatt tttctgtgct ggctaattta
     6121 tcttctggat aaacatccaa ggtgatatgt aaagttaaag tatctttatt tcggattact
     6181 ttaacgggaa cctgaactcc tggtttggaa gtggcaacag caatacgaaa ttttgataca
     6241 ttgggcactt tttcgccatt gaattcaata atcacatcgc ccacttgaat tccagcttta
     6301 tctgcgggag aatccttctc cacttttgct acaagcaccc ctgaaacttc ggaaaggtca
     6361 aaagcttcca ttaagtcagc tgttatttct tgtggtaaaa tacctatata agcacgagta
     6421 accttccctg tagctaccag gtcttctacc actcgcttag ctaaatttat gggaatagca
     6481 aaaccaattc cgatatttcc accgctggtg ctggtaatag ctgaattaat tcctatcacc
     6541 tctccattta tatttaaaag aggtccacca ctattaccag gattgatcgc agcatctgtc
     6601 tgtatataat cttgataaat aggagaattg gctccaaaat ttaaatttga ccttcctata
     6661 gcggaaataa ctcctaaagt aacagttcta tccagaccta catctccaaa aggatttcca
     6721 atagcaattg cccattctcc aatctccagt ttggaagaat ctcctaaggg cgcaatagta
     6781 actttctccc cttctttaac cttaatctta atgaccgcta catccgtgct tggatccaaa
     6841 cctactactt ctgcagtata attaatctta tctgcaaggg taacagtgat tgtcccttcc
     6901 ttaccttttt ccaccacatg attattggtc ataatatagg cttccctggt ttgtggattg
     6961 aattcataaa taaaaccact acccatagaa gttatctttc tctgcatttc cctcggaatc
     7021 tctggaaaga aatatttgaa tagagggtca tcaaagaatg gattggtaat tgcgggcaca
     7081 ttaacttttg cctctacttt tatctgcacc acggattctc tgacatcacg cacgacttta
     7141 actacaggat taatcccctc gtcattcaca aacattgagc tttgattttt ggaagtagac
     7201 agttgggggg gttctgtacc acctgtcttg gcaaaagcac aggacgctac aatcgctatc
     7261 aggattatag gtataagctt cttcccgatt ttcatagtat tctccttctc aatatgataa
     7321 taactttgta ctttctattt gtctaactta taaaaactta tacacttata tgaagttacc
     7381 tctctatctt ttgacgcatt gtggtcaaga aaaaaatcac tcttataaaa gtattatatg
     7441 cttaattata ccagtgaccc tgctgaattc ctgcagatat tgacaaaaaa tgtcatttca
     7501 acattatgtc tatatggata aaaaggacat aaagtcatat ataaaagata catccactcc
     7561 tgttaaattt attaagggtg taggagaagc acgtgccaaa cagcttgcta aaattggtat
     7621 taatacggta atggatttat ttgagtattt tcctcgtgat tatatccatc gtaaaattga
     7681 accagctatt tttgagctta atcctggaga tagcattgcg atgaccacaa tgatatcctg
     7741 ggtcaatgaa aaatctacca aaaatgggaa atatatgctt aatgttggag tgagcgatgg
     7801 acattctact ttaatctgca cctggttcaa ttatcctaaa gattacagcc aaaaattcaa
     7861 accaggaaat ctactctggg tttctggcac tctttcagaa tataatggac aactccagct
     7921 aatgcatccc gaaattgaat ttgtgaatga agaagaagag agtgatttct ggaagaagag
     7981 agaatacctg ccggtgtatc ctttaaccgg gaatctaact caaaaattct tccgtcgtat
     8041 catctataat gcttttgaag aatatgtgga taaaattgaa gaaaatctac ctgagtttgt
     8101 ccttgctaaa tatggctttc ttccccggaa aattgccttg caaaagatgc atttctccct
     8161 ccatccagaa gaacttgatg agattcataa aagatttgtc tatgaggaat tcttctattc
     8221 tcaactactt tgggcacgac acaaacatta tcacacggaa gaaattaaag gtatccgttt
     8281 tgagaataaa agacaactta cagctcagct ttataataag ctccccttta aactgactaa
     8341 tgctcaaata cgtgttataa atgagatttt tgctgatatg acctcggata aacagatgtc
     8401 tcgtttactt caaggtgatg tcggttctgg aaaaactatt gttaccctct ttgctatgct
     8461 tttagctgtg gaaaatggtt atcaagcagc acttatggct cccacagaaa tactggcaga
     8521 ccaacattac cataatatca ccagattatt agaaggttta ccagtaaaag tagcattgct
     8581 aaaaggcgga acctataaag gtaaagaaca aacaaaaacc gatataagag aaggaaatgc
     8641 tgatatcatt attggcactc acgcacttat ccagcgcgaa atctcttttg acaaattagg
     8701 ttttgttgct attgacgaac aacaccgttt tggagttcaa caaagagctg ctttagcccg
     8761 aaaacacaaa catccagact tactctatct aagtgctact cccattcccc gttccctttc
     8821 tctcactgtt tatggagacc tggaagttag tattattgat gaacttccac ccaatcgtat
     8881 cccagtcaca accctcatcc gttccagcca aaaaattgat ttggtctata aagaagtggc
     8941 aaaagaactg gaaaaaggaa gacaggttta tatcgtctgt cctctcattg aagaatctga
     9001 taaggttgac ctcttggatg ctcaaagaat gcatcattat atctccacta aaatattccc
     9061 ccaatactcc tgcattctta tccatgggaa aatgtcttct aaagaaaaag aagatataat
     9121 gcgtcgtttt aaagatggag aaataaaaat tctggtctct actacggtta ttgaagtagg
     9181 acttgatatc cctaatgcca cggtaatgat agttgaacac gcagaacgtt ttggtttagc
     9241 ccaattacat caactcagag gcagagttgg aagaggtgga caacaagctt attgctatct
     9301 tatagaacat atgcctgcga ctaatatagc gagagaaaga cttgccactt tagcctctac
     9361 tacagatggt ttcctcatag cagaaaagga tctggaatta agaggaccag gagaaatctt
     9421 cggcacagaa caatccggct tacctcattt caaatttacc aatttatttt ccgatcagcc
     9481 aatccttaaa ttagcacgta aggatgcctt ccagattata gctcaagacc cagaattaac
     9541 aaatccccaa aatagtttaa ttaaagacat ctactttcgt caatacgctg aaaaagaaaa
     9601 acttatcctg tactgatgag caactataat aattttaagg attgactaaa acatctattt
     9661 taattatgaa gtttataaac cattttcccg aggtaaaaat gaaaagattt attattatca
     9721 tctctcttat agtggcaata acagctatct ttgccattaa atttgatcct gattatttcg
     9781 tctcaaaatc tattatagta tgctttacta aaacagctat tggtaatgat actggaaaaa
     9841 ttgattttac taaaaatgaa ggtgtcgttc aaactggtat ctcctccttt gataagctcg
     9901 ctcaggagta tagaatagta gatatgcaac agatgcatcc ctatgttaaa gatcccacct
     9961 ggaatgacaa tggcttatat cttcaaaaca cctatcgctt aactttagcc tctgatgata
    10021 atatagatga agcagtggaa gctttgtcta atgataaaaa ccttatttac gctgaattgg
    10081 aaggtattgt tcgcagcaag tttgttccca atgaccctct tatcactcag caatatgtcc
    10141 atgatgttat caggagttat gatgcttggg attatatcca gggttcacat aatgttaaag
    10201 ttgctataac cgattctggt gtgaaatgga atcatcctga ccttcggggt aatatctgga
    10261 ttaatcctgc tgaagctcct ggtatgacta ttaattggga tgctggtact atatccggtg
    10321 gtgatggaat tgatgctgga gaaggtggaa ataaaataga tgacctggtt ggctgggatt
    10381 tttatgctaa tgataataat cctattcaga attatgcaag caattatcat ggcactcatg
    10441 ttgctggctg tgctggagca gttggaaata atgccatagg agtggttgga acctcaccaa
    10501 tagtttcact aatcatctgc aaaggcgctc cagattcctc tccatcacag ggtatttctt
    10561 ttgcctatga ccaggttaaa tatgctggag aagtaggagc agatatcatc aatgccagct
    10621 ggggtagcgt tggaaatgga gcttacccca attctattgt taattatgtt accaatctcg
    10681 gtgctttagt ggtcactgcg gctggaaatg aaaatactga acataatagt tcttatcagg
    10741 tttatcctgc agattgcaca aatgcattct gcgtggcatc aacgggacgc aatgatataa
    10801 aatccagttt ttctgattat ggtgcaacca ttgatatctg cgctcctgga gaaaatatcc
    10861 tttctacaat tatcgctaac aacggttatg aatccaatag tggcacttct atggcttcac
    10921 ctattgtcgc aggtgtctgt gcattggtta aagctctaaa tcctgaattg acccctcaac
    10981 agttaatgca aagagtaatg cataccgctg attatattta tgacaaaaat cctgattatg
    11041 taggaatgct tggtgcggga agagtaaatg cctttgccgc tactatgtat gataagattc
    11101 cctatataac cattgaagat tactctctgg aagaaatcca gggtgacggt gatggcattc
    11161 ctaatcctgg agagactata aagttaaaag tttccttgaa taattatata gacccttaca
    11221 ctggtcttgc ctggttggat gcacaaaacc ttagtgttac tctttctact acttatcctg
    11281 gggtcacaat tatagaaaat actgctaatt atggaactat gattgccggt agtactatgt
    11341 ggaataataa tcaacctttc atttttcaaa cagtcggcac attaccttca gaacctattc
    11401 cttttgaact aacattaacc gccaatcaat ctgctgatta tccctataac agagtccttc
    11461 ctcttaatat accactcact ttgaatcaag tgggttggcc cctaaatttc agtggagctt
    11521 ctacttccag tcctataata tataatttgg actctaactc ggataaagag atagttgttt
    11581 cagaaccaac cggaattgta catatcttaa aacctgatgg aagaaccgaa ctggaaggat
    11641 ttcctctcaa tttgggttct gccatcattg gttcactggc aatgggagat gtgaattatg
    11701 atggtacttt agaatttgca gcctgtttgc aaaataataa tatcgtgctt ttcaatcagc
    11761 acggaaccat tctctggtcg gttccctcag gtggaattct tcgtaatgga cctgttatcg
    11821 cttctcttgc tatggatgat tctcagcaaa taattgctct cacccagact ggaaatattg
    11881 ttgttctcaa tccggatggc tctgcttatc ctaatttccc tgtttcagcg cagggtattt
    11941 gtcttggaat tcctgctatt ggtgatttaa atggagatgg acatcatgaa ataattgtcg
    12001 caactatgac tggcaattta aatgcaatca atagcactaa tgctcaaaat atacccggct
    12061 tcccggtttc tatgcaagga ggttctcaaa atcctataac catcgctaat ctggatgatg
    12121 ataattatcc tgaaattata gttacaacta ccgctactgg taatgttctg gcttataatc
    12181 acgatggaac cttgcttttc caaaaaaatt tggggggaca aatcaagact ggtgctgtgg
    12241 tagctgatgt ggataataac ggaataatag aaataattgt gattactgcc tcaggcaata
    12301 ttaatgtact cactccttct ggagcagatt atcccaatac acctctttct atcaatcaaa
    12361 ctgtagaatg cactcccgtt gtagctcggt ttgatggtga taactatgat ggaattattt
    12421 ttggagattc cgatggctat ttgcatagcg ttagagttga tggcactgaa tctcctaatt
    12481 tccctattcc gattggtggt aatataaaag tatctgctgc tttgagtgat atagatagag
    12541 atggggatat tgatatcgtg attccaaacg atgaatctat ctatgtaata gatgtgaagc
    12601 gtcccgctca atcttatgaa tggtattgct atctgggaac ctggaatcgc agtggaaata
    12661 tctatcaagc cactcctaat aatgattata ctgttcctca attagttact actttgtatg
    12721 gcaattatcc taatccattt aatcctgaaa ccacaattag atttgaatta gctacccctg
    12781 gtctagtaac tctggatatc tacaatcaaa aaggacaact ggttaaaaat cttgtaaata
    12841 ctgaataccc tgcaggtttt catcagattg cctggaatgg tacagaccag aagggttcac
    12901 ctgtcagcag tggtatctat tactatcata tgcgttctgg aaaattctca gccacccgca
    12961 aaatgatatt gatgaagtaa atatataagc ttaataataa attaaaagca tattcttttt
    13021 ttagtaagag gaaagaatat gctttattta tttataatta gggtttcact taattaataa
    13081 cttacctcat tcctatcttt ttattcagga attaaccgag atacagtcag atatagtgtt
    13141 acgctgtttc ttacacttac taatttaaaa atgattaaca aacgaaagat aaattaaaag
    13201 tccaatgaaa ttggaattga cataaaaagg tgctaaggaa aactggcaaa tagaatagat
    13261 ggtgctactt gataggtaaa agggaatctc gtgaaaagcg agagtggaaa ccgccactgt
    13321 aagcagctca aaggctgaat cagtcactgt tttaaaaaat gggaaggctg attctttata
    13381 gccgctgcaa gccaggaaac ctgccactaa taacatttct gttatacggt tgcattaaca
    13441 gaatgacaaa gtagttcatc cctattcaat attaagaata aggtgaacca aatgaaacaa
    13501 ctaaaaatca cacttctggc agtatttatc tgcctttttt ccatctcctt gtatgcaata
    13561 gaattatttg tagtcaattc cgtctcacgc actctttctc gcatagatac tgagaccgga
    13621 acagttaata atacctttgc tcagttaggt ctaactccta accttatgga tatggatgat
    13681 aacaatatct atgtggtatg ttctggagat aacgctattc aggttatttc acgctctacg
    13741 ggcaacagtc tttattttct tccgatacaa tcttccagca atccttatga tgtgctcaag
    13801 gtgggagatt atctttatgt gacgggctta tttaccaata aactatacaa agttagccta
    13861 ttaacaaata atgtggtctc ttctctaaat gtaggaattg caccggaagg actttgttcc
    13921 gatggttcaa gactttatgt ttgcaacact ggaggttatc aatttaatta ttcagatagt
    13981 tctatatccg taatagatct caattccttt tcggtgatag aaactatccc tgtctggact
    14041 aatcctcaat ttgcctgcat ctatggaaac tatttacaca tagcttgcac aggaaactgg
    14101 agtaacatct caggaaagat agacattatt agtctggaag acttttctct ggtgcaaaga
    14161 cttgatatag gtggacaccc tggaaatatc tggataaaca accttggtat agcttatgtg
    14221 ggagaaggaa tgagtaatgc actctatagt tataatgcca gtacctttga aatctatcat
    14281 agtgcccata atccattaaa ttataacgct tttatggtgg ctggtaatca agaaataata
    14341 gctttgttaa atcaaaactg gacttccaac tctattatta cggtctatga ttatgatttt
    14401 aacttattaa acaccttttc tgtaggaatt tcctccactg acattatggt cgcaccttca
    14461 gtatcatctt tagaagatga atgtaatata gtcccccagt taaagattta tcctaatccc
    14521 tattcttttg gaaaagagtt acatttttca gataaaatag cagattccag tcagctattt
    14581 ctttataata taaggggaca gatagtcttt cagacacaaa taagagaggc ttctccaatc
    14641 attattccaa atgaacttac tccggggatt tatctttata gaattattca gggaacaaag
    14701 aaggattccg gcaaattaat aattaagaat taatagagtt cagattttcc ttgactttag
    14761 gctttttaaa tagaaattag attaatctaa aaaatagata ggagttctca gatgaattat
    14821 tttctaaccg aagaacaatt agagatgcaa gaaatagcac gaagaatagc taatgaaaag
    14881 atgaaacccg tctcacaaaa atatgatgag gaaggaatct tcccctggga tatagttgaa
    14941 gtgatgcgtc aaagtgacct ttttgccatc ctggtaggag aagaatatgg cggaataagt
    15001 ggaaaagtta tggatttagc cattgtcacg gaagaacttt gtgccgttga tatgggcatt
    15061 tctcttgctt ttggagctac cggtttgggt atgtatccca ttttgattgc tggttcagag
    15121 gagcaaaaac aaaaatatct aactcagata gcggcaggag aaaagcttgc tgctttcgct
    15181 cttacagaag ctaatgccgg ttctgatgct ggagcaatag aaaccactgc tcggaaagaa
    15241 ggagaccact acattctaaa tggaactaaa caatggatta ccaatggtgg tgaagcagac
    15301 atatattgcg tctttgcaat gactgataag accagaggtg ctcgtggttg ttcctgtttt
    15361 atcgtggaaa aaggaactcc tggtttcaac tttggtaaaa aagaaaataa gatgggaatt
    15421 cgtgcctctg ccacgcgtga actcatcttt gaagattgca tcgttccagc agaaaacctc
    15481 atcggaagag aaggtatggg ttttattata gcgatgaagg tctttgataa atctcgtcct
    15541 atggtcggag ctcaagctgt tggtgttgcc agaggtgctt ttgaaactgc tattgcctat
    15601 tccaaacaaa gacaacaatt tggtaaacct atctcctcat ttcaagctat tcaatttatg
    15661 ttagctgata tggcaactga gattgaggca gcaagagctt tagtctatca aactgccaga
    15721 ttgattgata gtggtgctaa agactatagc aaagaatcag ctatgtgcaa atattttgct
    15781 tcagatgtgg caatgaaagt aaccactgat gcggttcaag ttcttggcgg ttatggttat
    15841 atgaaagaat atcccgtgga aaaaatgatg cgagacgcaa aaatcttaca gatttatgaa
    15901 ggtactaacc agattcagag aggcattgtt gcatccgctt tgcttaaaca gtattaaatg
    15961 ctaacctttt ttaagctaaa atggaaactt tagctttccc tgagcatctt ttattacata
    16021 cttgttgtgc accttgtctg atagccccat atcatcagct taaagagcaa ggcttatctt
    16081 tcacttcttt ctggtataat cccaatgtgc atcctgtaac agaatacaga atgcgtctac
    16141 agactttaaa aacctttgct gaaaaagaag atatacctct ccttttgcat gatgattatg
    16201 gattaaggaa ttttgttaaa gctgtggtag atgatattga tgatagatgt agacattgtt
    16261 ttcgcactcg cttagaaatg actgctcaaa ctgcattaga gcttggctat gacgcttttt
    16321 ccaccactct tttatatagt atctaccaga aacacgacct aataaaagaa attggtgaag
    16381 aaatagcaga agagaaacat atcccttttt tctatagaga ttggagaact ttatggtcag
    16441 aaggagttcg tctttctaaa gaagcagaac tttaccgtca aaaatattgt ggttgtatct
    16501 tcagtgaaga ggaccgttat ctaaagagaa aaaatgagca gatataatga aatagacctt
    16561 tctaagattc attttgcctc agtccagaat agatttagca aggtagacac ttcttccttt
    16621 gctcaggttt ctgataatct actggatagt atgccagaca ttctcaaagg tgccgagttc
    16681 aaagaattta tcaagcgttg tcacctggca cgagattccc ataaaacttt tattataggt
    16741 ttaggtggac atattattaa atgtggatta gcacctttgt tgataaaact tatggaagaa
    16801 gggatagcta aatgttttgc tgtgaacggt tccgtcacca ttcatgattt tgaacttgct
    16861 tattttggaa aaacttctga agatgtgagc aaaggtcttg cagatggcac ttttggtatg
    16921 gcagaagaaa ctataaatgc tataaaccgc attattatcc gagccagcga agaaaaactg
    16981 ggttatggag aagctttagg aaaggatatt ctggaaaatg cacctaatgc agaaatttct
    17041 cttttagctc aagcttataa atataatatc ccagttactg tccatatagc tctgggaacg
    17101 gacatcactc atcaatcacc ctatgcagat ggtaaggcta ttggtgattg cagtatgagg
    17161 gacttccgta ttttctgtga gcaagtcaaa aatttaaatg atggtggagt cttcatcaat
    17221 atgggttctg ctgttataat tccagaagtg ttccttaaag cattaaccgt tgctcgcaat
    17281 gtctatggga caattcaaga ttttactact gcagtgtttg atatgaatca acaatataga
    17341 gagttagaga atgtagttcg tcgtcctgta gaaaaaggag gaaagggtta ttactttata
    17401 gggcatcatg aaattatgat acctcttttg gtgcgggaac tattgcgtta agcttttcct
    17461 ttgttagcct aaagccacat ccagaaccat cataaccaca aaaccgagca tcaatccaag
    17521 cattgctaaa tgggcatatt tcccattttg acagcgtggt atcacctctt ctgccacagc
    17581 ataaatcatt gctccagcag caaaagaaag agcatagggc aaaaatggct gggctatcaa
    17641 tactaatgct gctcctatca ctcctcctat aggttcaact gctccactaa tctgtcccca
    17701 gaaaaagctt cttccagctg aaagtcctgt tttccttaag ggtatagaca cagcagcacc
    17761 ttctggaagg ttttgcaatc caatacctat ggtcaaagct attgcaccag ctaaggaagc
    17821 agtttccaaa ccataagcta cagctccata ggcaacaccg acagctaatc cttctgggaa
    17881 attgtgcatt gtaactgcca gaaacaataa agtggattca ccccagggag ttttaagtcc
    17941 ttcactggag caagtttctt cacttaaatg ctcatgtggt agcagcttat ctgcagcgaa
    18001 aagaaagaga cccccagcta aaaaaccaat tgcggcagga aaaggattac cttttgtcat
    18061 ttctatagcc ggatttaata atgaccaaaa actggcagca atcattactc ctgccgcaaa
    18121 acccatcatt gtatccataa accaggtctg gattcttttc acgaagaaca caagactggc
    18181 acctgcagca gtcattaacc aggtaaagat ggttcctatt aatgccagaa ggataggatt
    18241 gagactttcc agattcatat caagaataga aattctctat tgcttcaagc atctgctgaa
    18301 tagcagcatt agggtcatct actgctaagg ttcttttatc tccattgtca aaagttatta
    18361 ctacacttcc taacaccttg gatatttctc gtatcttggg aataacgata tagcttttcc
    18421 ccagatgctc atcatcgtat gtatatattg ctttcatagc ttataccttt ttaaattaat
    18481 ctatatcttc atctggagaa gtcaaaatca tcctccagtg aagttataaa agcagcaatc
    18541 agttgaacag cagcatccaa atctttaaaa gatatcacct cattaggaga atgcatatag
    18601 cgattgggaa taccgataac cgccatagca gtgccagatt tcaggtcttg aatgacatct
    18661 gcatccgttc ctgttcgtcc tggagcaatt tcaagttgat aaggaatatt aagttcttta
    18721 gccagagcaa ttagtttatc attaatagca ggatgcattg ctgcaccgag agttatagca
    18781 ggacccttac ttaaagagat atcgccaaag atacgtttat ctgctcccgg aatatccgaa
    18841 gtaaaagtca catctaccgc tatggcaata tcaggttcaa tttgccaagc actggttctt
    18901 gcacctcgca tagtggtctc ttcacccaca ctggaaacgg catagtaatt taccttaaga
    18961 tttttatctt ttagctcttt catcactgca gcagcaacat agactccgca tttattatct
    19021 gtagccttgg agactaccag gtcatcagag agcatttcaa attcgctatg ataagtaata
    19081 atatctccta tagcaacttt attttccgct tcttctttag aaagcgctcc gatatcaatc
    19141 cagaggtctt ctaacttcag tttaggaggt tcttccgttc cgcgttgcat atgtataggt
    19201 tttttgccta taatgcctct taccggtttg ccttgatgat aaatatctac tctcaaacca
    19261 ggaagcagat tgacatcaaa accaccgaga gtgcgaacat agacataacc ttgctcatct
    19321 atatggttaa ctatgaaccc aatttcatct gaatgtccca ctaccataac ttttagtttt
    19381 ccacttccct tctttaaagc tatcacattg ccattacggt ctttatttat ttcatccgca
    19441 taaggaatta gaaagtccat taccactttc tgagctggtc cttcaaaacc ggagggactg
    19501 ggtgcagaga caagattctg gaagaactgc ttattctctt cagataacat tttttctcct
    19561 aacaattatt agcagtttct tctgcaaagg gattgatgac ctttttctga acgggatgtt
    19621 catgtggaat aatacacaag aatttcagaa catcttttcc ggtattttta aaagtatgta
    19681 tcattcccgg gtctacatag atagcatcac cttcagcaaa aggcaattca cctctatcag
    19741 ttttcaaagc gccagttcca ctgagcacat aaacctcatg ttcccaatca tgagtatgat
    19801 aaggggtgtg tccaccaggc tcaatttcaa acatacgcat agcaaaattg ggagctccat
    19861 ctttttgagc tataagccaa cgaatttctg ctcctttggc accttcagca tttacaggtt
    19921 ccaggggcac ctgatcaaaa tggataatct tcatttattc ctccattatt agattatatt
    19981 tactcatctt tttaacaata tgataaacgg ataaaaaaga aaagctatga ataaactggg
    20041 tagccaataa ccagccaatc ctcacctgtt ttttttagtg taatatcttt caaatggata
    20101 gcagaattga gatcgggcaa atttaattcc cttaatattg ctttattacc tcccaatatt
    20161 ttaggagcat aaaatatata gcttttatct actaatcgtt cagaaaaaaa ggaagaagct
    20221 aactgacttc cacattccag tagaaccaga tagaatccct gttgatgaag aatccctaaa
    20281 acctctttaa ggcagagagt tctcccatta gccgtgacag ggtataggtt tgctccaaga
    20341 cttacaagag cttctgcatg cggtgaattt tctttccctc tcgcacaaaa tatggcagtt
    20401 ttatattctg gcagggaacg aactatctga gaattgagcg gaatctccag atagggatcc
    20461 agaatggctc ttaaaggttg tttaataggc ttgggcaaac gaacatttaa cattgggtca
    20521 tctttcaata ccgtccctat accagttaaa ataacatccg cttcttctct taatttatgc
    20581 accattttgc gagaagattc tcctgtaatc cagcgagaag aaccatcctg ggcagcatat
    20641 ttaccatcca gagaaagggc agctttccag agaatgaagg gtcttccttt ctgaattctg
    20701 caaaaataat attccagttg tcggtttatt tcttcctgca taattcctga agttacctca
    20761 attccagcat ccagaagctt ttgccttccc ttaccattaa ccagaggatt ggggtcttga
    20821 ataccaataa aaactctttt aattccagat tttattatag catcagtgca aggaggcgtt
    20881 ttaccataat gagcacaggg ttctaaggta acatacaaat ccgcatcccg agcagattta
    20941 cctgcttttt ttaatgcttg aatttctgca tggtctttac catatttttg tgtccagcct
    21001 ccggcaatga ttttcccatt tttaactatt acggcaccca caaaaggatt aggagagcag
    21061 gttcctcttg cattttcagc aagtcggata gcctctttca taaaattaac ggggtcagtt
    21121 ggaatatttt tcaaggtttt tcttggctgg ttcatagttt ggtgcaattt gcaggatttc
    21181 ttgccacata ttttttgcta actccagatt tcctaagcca gcttcacagg cagcaagatt
    21241 attccaggca ttaagttcat caggtttttc ttgatggaga aaatttagca ggtctcgtgc
    21301 tggttctaat tcggaattat gtagatacaa agttgagaga gcaaatccgg cagcaaaaaa
    21361 gtgattatcc agagcaagtg cattccagaa atcctcttta gctttgttat attgctttgc
    21421 cagttgccag taaagtgcac gataataata aaggtcagca cagtcaggaa attttggcat
    21481 atttgatgtt aagagattcc aagcttcttc tgctcttcca ttggcaaggg catccgccag
    21541 attctgaaat agctgattct gttcataaaa cagatgtgga ccgccattaa agttttgtct
    21601 tatcttccat cttcctgttg cagaggtaag tagcactgcc caaataattt tattatttaa
    21661 ttccagaact accatagcac taattccatc gtcagcagct ccagcataaa gaatagaata
    21721 ggatttagtt ttcttcaatt ctggaattga tgaaaggatt gctgtatagc gttcagcgaa
    21781 ttggtcaaag tccacatcgt ttatagtaaa tgagcgtaat ttctcccatt cctgtgtaat
    21841 aacagcatca aaaaaggcaa aggttaccgt ttccggagat tctggttccg ctatttcttc
    21901 ataaggaata ttcaactttt gcattaaata ggaaagagga aaatttgccg gatacttgta
    21961 ttgctccagc cattttagca tcttttcact atcttgtcgg gtaaattctg ctggacttaa
    22021 accatccagt tctggaagac ttttatagca ccagtaatcc aaaaattttt tagtggtttg
    22081 cacaaactct gtattggaat cagaacgaaa agcaggaaag ggactggtgt gcgattcatc
    22141 aaattgagcg gcataaggac aagaggaagg acaatgtaaa tctatcctga gattcataca
    22201 acatttccaa ccaatcagtt tatctttata gcgaggacat ttacgcactg ccctttcaga
    22261 gtgacaacga aaacatcgtt ctttactaag tagcattttt tatgactcta aaataggggg
    22321 cagacgacgt ttgaaagcag agtattgaat cattctttcc actttttgat aaagttccac
    22381 gggataggat aggttctcgg tggcagaaat acttaaatga agttctgtca attcataaac
    22441 tatctcatcc agaacggggt aagatatacc tatttcttgt tcatcagatt gaccgggcca
    22501 aaggtctgcc gtaggagtct tatttattat tttttcggga atatttagaa tctttgccag
    22561 tttccagacc tctgttttgt aaagatgacc gataggttca aaagcacaag cagaatcacc
    22621 aaattgagta aaatagccta tcataagttc tgttctatta cttgtcccta aaactaatgc
    22681 cttcaatttg gcactaaaat cataaagaac acacattctg atacgagcaa gccagtttcc
    22741 aattcgcata tggtcagctt ccggagcata agtttcaaag taggctttag ttatagcagt
    22801 tatatcaata atctgataat ttattccgag ctgttttgct aaatctctgg catcagctac
    22861 agattcggca ttgctggtct catagggcat tattaaagcc gtaacatttt gggcaccaat
    22921 agcttcaact gccagagcag tacttacagc agaatctact cctccagata atcctaccac
    22981 taatctatct ctttctgcat ttgataaata gccacgaata aattcaatta tcctttcttt
    23041 ttctttatac aaatcaagat tccgcattct ttctccctat cttatttgtt ttattctaaa
    23101 aatttctcct ctaatttcag tcaaggataa atccatagga ctataaggca ggataaagaa
    23161 ataggtaatt gcgttagaat aaattagctg ctttaggcat agctcattcc cactctatga
    23221 tatataattt atgtccaact gatgtccagc ttatgtccaa ctcatgtcca actcatgtcc
    23281 gaaatcggac ataagctcta tataaactgg atatttatgg gataagaaca agctaaggaa
    23341 ctaataatca ataatggtgt gcagatgtcc tcatctacac caaaaaatat tcagggatta
    23401 gcgagtgcgc agactttaaa aaaagcttga gaattgtttt ctgaatctaa gataaaaaaa
    23461 ggattgtcag aagagccaag atattcccat attccaccgg ggttcaatga ataatagata
    23521 ttataggaga tattataaat cggagctcca tccaaagctt gattaacagg gtcccagctt
    23581 agatataata aatcagagtt cttaataatt ctaacattct gcggaacctg aggaatgctc
    23641 atatttctgt attcataagc tccaatatca atataatttt ccataatacg agggttgcct
    23701 gtaaggtcaa aaagataaag cgaaatatct gtaaatggat taccgctatt cctacagggt
    23761 gaattctgat gtaatcgcca atctgccgct aaaccatcca gattcaggct gttatcaatg
    23821 gttggttgaa taaattgtgg atttccagtt atattatgat taccgtattg actgtcttgc
    23881 cagggctcat tttcccagct aatattaaaa gctgccagac cattttcaat atcattatag
    23941 tcaaagaatt gactgggtcc gtcggttctt atgccaattt gcccctgaga actgctgttt
    24001 ccccagataa tattattgta gcaatctgca tagcctctaa tatacaaacc tccaggacct
    24061 ccattgggat aatttatggt agcagaatta ttacagatgg tattatttat aatagaattc
    24121 ccagaagcat aagatgaagc aaaaattcca ccaccttgat gagaagttcc attattgaca
    24181 ataagattat tcaagcaaag ataatgacaa ttatttttaa gagcaattcc acctccgcca
    24241 ttttgagcat aattattgga aaatttactg caaatgatat aatgacttcc tccacaaaga
    24301 gttataccgc cacccatttt tcctgcactg ttagatttaa aggtgcaatg ttctatatag
    24361 agagaagtct gattgttcaa atctggcaaa gaagtaaaaa tagctcctcc atcataagtg
    24421 gctgaacaat tatagaaaaa acaatttttt actttaaaac tactattctt ggcatataga
    24481 gcaccaccac tatcagctgt ggcataacta aaacagcaat tttctataag taatctggaa
    24541 ctattcaaaa cccggattat gcctccattt ttgtcctgac tatcctgtct cgcatctccc
    24601 ctgcttaaag aacaataacg aaatatagta gaatcattgc tggaagctat gtcttgcata
    24661 agaattccac gccacttttg tgaatttgat gcggtgctga atttaatact atcggagaaa
    24721 gtgcctatag cctgtaatct accctggatt tttaaagtat aatatccctg aaattgtaat
    24781 tctactccgg gattaagtgt taaactttct ccctcaggaa tgattatatt gccattaatc
    24841 agataagggg aacctgcaac actccataca ccactgacat tccctcccga aataactgta
    24901 gctgcagata aaaaacaaga aaaaagtatc aacaaaacac tgcacagaca ccgcatattt
    24961 ttgctccttt ttccaatatt ttacaatcct ttaaaaagat aattatattt ttgtcaacta
    25021 aattttgtag tttgagaata agcgatttac tcattgaaaa aactgactta agacttccag
    25081 actgcgaagt tttagaggtt ttcggaagtg gttttgataa tggtcagaaa aaagatggtt
    25141 tagctgttcc accgtttgac tatctggatg aattttatcc aggtaatcac caattacagg
    25201 aagtagatgt agaatttgag atgccgaaga agttaatacc tcataatatt caggataatt
    25261 tgcttctttt aagcattcag cacagattaa tctacctgtg cctttttccc aagccactat
    25321 ttgcagcttt tttttattac aaacagcaca taaatccaga ggaaaagcta tacccagcat
    25381 cctaaatatt cttaataaaa aacgccacca gagtaagata gaattatcac ttctgatttt
    25441 caaatagtcc agatagcttt gtaataaatc atagtaagcg gggttttctt ctgagggaat
    25501 aattatctgc gtataaagtt ctgcagcaca ttcagccgca gtccaagctg gtatattatc
    25561 aaatagatta aattctttaa ggagagtaaa ttcacttaat agatacaaac ccgattcttg
    25621 aggctcataa agggtaaaat cgtaggtgta aagagaattg agaagattat tttctggttt
    25681 ttttcggatt cccttagcca gaatagatac caatccacga tatttgagaa atactctaag
    25741 aatgaggcta ctttcacgga attgagtagt tttgaacagc acaccctgat ctttaatctt
    25801 acctttcaca gatttaattc cttttttgcc gttcttaaat ccataggttg cacaaatgca
    25861 gttttatgat tggtataagt aacaaaacca ggtttgctct tatgaggttt tcgcacataa
    25921 cgcacctgcg tgtaatcaac tggaacattc tgagaaaaac gagctttact ataccaagct
    25981 gcaagagaac aacaaatctg aatcaattct tctggaggat tggttttctg tagacacctt
    26041 aaaaggatat gcgaaccatg ataaagacga gaatgaaacc accaatcgtg aggtctggct
    26101 aattgagtgg taataaaatc attctcgctg gcttttcttc ctatcacaat ttcaaaacgg
    26161 tcatcaatcc taagacgaag cagcttttct gccatttcca atttacgaga taattggggc
    26221 aacttttcct tttgaggtaa ttcatccagc tcacctcttt tagctttctg gacaatgttt
    26281 tccagctgct taatcttttt ctcagtttcg gcgatgttat gttctataac ttcctttcct
    26341 ctctttgctt ttttgtactt tttaagatag tattgcatat tttcctgagg tgtcttgtcg
    26401 ggcaaaagtt caatttcaat agtagaaagc tcagggtcaa aataatttat tgctttcagg
    26461 acattctgac ctctctgaat attagacaga ttaactttta tgatttcagc acagattctc
    26521 cagtaatcgc ttttttctgc ctgttccagt tccattcttt gtttatgaag cttttgttga
    26581 gtttttttta attctctttc ccagtagtca acaatagctt ttctatgctc cagttcttcc
    26641 ttaactgaaa gaacatagat aaagtggttt tttaagtatt catttacggt atcgcactga
    26701 attgtttctc cagtctttaa ggtttgtaag attaagggaa aagaaatatc tctatgataa
    26761 ggtttaaagg cggttttggg tggttgatag ggtattttgg gaagaatctg tctttgagga
    26821 ttatccgcat aggaatattt tcttaaggca tctacgatga ttaactcctg gttttgctct
    26881 gctaaaatta tatttggttt aggagggata aattctgcta ttaggacata agtttttttc
    26941 tgttggaata tatcaatttg ggtaaaaata aaacgcataa tgcggtcata ctcgtctaaa
    27001 ctaacttttg ttaagacaga atgtatcaat tggtcccaga taggggtttc atcaccgata
    27061 gaaaaaggct ttgaggtata aaaaggatag gcatcataat tagagataac taagtagagg
    27121 tcagtattat ctttgaagtg aatgcgtaat gctttgtcgt tgtaaaatac gcattctaca
    27181 atctttttct caactgaaaa ttccttcgtc caatcaaaaa gaataatata atccatatat
    27241 tttttcactt tgtccttgac aattaaatta ataataataa tttgtaatat aataattgaa
    27301 tccattaagg agtcaaggat gaataagaca acttggatca taatagttgt agtgctggca
    27361 atacttgcca taatatttgg tgtaatgtgg aataacgcat ccgttaaagc caaaaagctt
    27421 gctcaagcgc aggaagagct gcaaaaatca attttagaaa aagaggaacc cgtaacggca
    27481 gaagagttac aaaatagcat tgatgccttg ggtaaagaat taggattaat tggtgctgga
    27541 acggaatttc ctgtaggtac accagaggaa cttaaggctc gcaatcttaa caccttaaat
    27601 actgcccgga ccaagattgc cgaatataag aaacaaatag cagacttgga atccaaaatt
    27661 gctgccaata aaaaaactat ggcgagtatg caaagtacgg tggatagact taagaagtct
    27721 cttgctgaaa aagaaagaat tgttgcagaa ttagaaggca gagtttctgc actcaatggt
    27781 acacttgatt ccgaacgcaa agcagctcaa gaggaaatcg ccaaaagaga gaatatgctg
    27841 aaagaaaaag agagtgagct gaataaggtg aacattgata acaataccct ctactatgct
    27901 atagggactc gtaagcaact tcttgatagt ggaatcatta ctcgtaaagg tggcattctt
    27961 ggtatcggca aagtgtccac ccttaaggat gtaaatttaa ccaagttcag tcaattcaat
    28021 cttctggaaa ctcaggaaat aaccttccca gttaccaaga aaggatattc tgtgctttct
    28081 aaccatgtgg cagcttctta tgaagtgaga caggaaggag accaatatgt gcttacaatc
    28141 actgaccctg aactatttag aaaacagaag gttttagtga ttgaactgaa nnnnnnnnnn
    28201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ttagtgattg aactgaagta actctaaaat
    28321 agtacaagtc ttaagccatc cttcaaagga tggctttttc tttataaatt cagatattta
    28381 gctgcttcaa aaagagctct gccacctaca aatccaacca atgcctgaac tatattaacg
    28441 ggtaaatcag ccagtgctac agctttaccc agttgaggca gaaaccattc tcccataaaa
    28501 taaccaacaa ttataactat tgccccaatt aagggaaata ttatctgcca gaatccagat
    28561 ttctgatgag ccaaaccaca aatgaaccct tctaaacctt taacgattaa agttatagga
    28621 gcaaaaagtg ggaaaagcaa aaggtcagct atagcagaac ctataccacc tgcaatagca
    28681 ccagcttttt ttccagcata aagccctata aacacaatca ttacatctcc gaaattgaaa
    28741 aaacctccac ctggtacggg aataggtata atcattgtgg cgactgttac catcaccaga
    28801 aatccaagaa tcaaatacca ctgattggga ttatacttct taacttgatg acagtgaatg
    28861 atgcgattat caatatattg acccttatga cttgcttccg tgattaaaga aagcattagt
    28921 gcaagaacag aagaaagccg cgccttaaat ctatgggaaa aagggagctt ttcatattgc
    28981 tcagacggga tgagactttg atgaaaggta atttcttgat aggaattgat agctgtatta
    29041 gatagcacct tccccaggat aacatacttc atcgtaccaa tttttaaagg taagtgcaaa
    29101 aggtcattga aacctacgat ggagaaaaaa agttgaaatg ccaggactga atttacaaaa
    29161 agcaaaatcc ttatactgcc gaactccaac cagaattgaa aagaacctgg ttctccttct
    29221 ggggtgtgta acctaaagag cccgaataaa ctgtagacta tgattaggga aaccagaaaa
    29281 ggcagcatta atcgcaatcc tgcaagccaa cgttttccac ccttgcttaa tagtagcaaa
    29341 acagataaca ggaaaacaaa tgctaactgg acggcaagat tttccatata gaaaaatagg
    29401 actataacca gacaggctac aagctgaagg aatgcgaata taagtttaat tgaaggggac
    29461 atatattttt gaaaaagatg gagatacggg aataaattcc agagtttttg ttgccggtaa
    29521 aagctctgaa tgtgcagaat aaatcagaca gtaatcaggt atatactgtt ttataaaatc
    29581 atatatcttc atttcatatg gtgattcatc cataactata aaacaaggac gaggttcctg
    29641 actataattg aaaagaagat tattcagcag ataatcaacc gtatccatct cattatgaat
    29701 ctcttttctt ggaggcataa tgctggcata agccttaact gcttgaatct ggaaatgcat
    29761 ttgctgctca aaataaagaa gaaagatatc cttttgatta agaagagaag ggataagtag
    29821 cctttcaata aaagaacttt tgccacttcc gttagcacca tgaagataat atagtgccac
    29881 agaactaaag gttaaatctg aggttagctc cagagaaaaa tcatcccaga caggataagt
    29941 cccagattca agtctgatca ccaatttcct cctgtctaat ctctttttct ctggaataca
    30001 cttccagcaa atcttctatc ttttgcctat ttctttcatc caaagaacgc cagaggtcaa
    30061 taaaaaggat ttttttagcc ggagaataaa gtgccaatag acacattaat aagactttct
    30121 gtcctccaga gagagtgcct atctcatctt ctggtcctaa atgctttcct gttatgtctc
    30181 caaattcctg agtcagttct ttccatttct cagttgagat attatataac agcaattctt
    30241 ctctcagtaa tccttcactg ataagtggtg aacgagcatt gcagattaaa tattcattaa
    30301 aatacttttg cagcatagga ttatggcgga gagtcaggga tttgaaccct gggtatccga
    30361 aaggatacaa cggttttcga gaccgtcccg atcaaccact ccggcaactc tccttatata
    30421 tttcttttat tttgaaaaaa atcttttaat agtgcagaac aatcttcttc aagtattcca
    30481 cgaataactt taggatgatg attaaagctt ttatctgcca gaacattgta aacagaaccg
    30541 actgctcctg ctttgggatc gtaagcacca taaactaagg ttcctaatct gcttaatatc
    30601 atcattccag cacacataac acagggttca agcacaacat atagcgtata atcatataaa
    30661 tacttgattt cagaagatag tatcctgtca attatcattt tctctgcatg ggctaaagga
    30721 ttgcaatatt ggcgagtccg gttatgttct ttaaggacaa gtttattatc cttaattagc
    30781 actgcaccta caggaatttc atcttccttc agagcctgtc ttgcttcttc cagagctact
    30841 ttcatccaat taatgtggat attatcttcg tccttcataa tgttagaaag ctgcgttgat
    30901 gctaatggaa gtgcgccaat ccagattttg aaggtcctta ccaacaggat tgatggtaaa
    30961 ttccagaaga gaagagcctg gtaatttcca gactagtcca gcttgaaaat catactcttg
    31021 ccaggttccc caagaatatt ggggctgaag ataatagtaa aaatcataga tatcatgctt
    31081 gagcgttaaa ccgagagtat aacgattatt tctaaaattg ataacataca cagaccggtc
    31141 ataatcagca tattcattct gttcaaaacc aaagtttata gaggtctgaa acgcattttg
    31201 atgatgatgc ctataaggaa taagcaacca gcgttgatca ttggaacggg caataaaggg
    31261 ataattatca tagaccagct tgtaatctcc tttcaattga cgatagaggc ctttttcaga
    31321 aggactaaac acataatcag tataatcagc acggataaca tagttctggt caaaaagcag
    31381 acaatctcca atcaaaatag cacattcagg attatatata taactggtgg tttgcttatt
    31441 atttcctgaa agaatgctgt tcacatagac atcatcagtt agattccagt aaaagcgatt
    31501 actgagtttc aatctatctt tccagtaatg gacattaata agagagcaac ggatattacg
    31561 caagtcatta tcccacttat gctggtcttt ggggaaactt gctctttgca aatctactga
    31621 aattcctgcg gaaagggtgt ctccttgtat atattcccaa ttcaaaacgg tagataggaa
    31681 acgattttca ttttgacggg aattttggga aagattataa tcttttgtca gatagctatg
    31741 attgaaaata gtagtccatc ccacttgatt ccagggtaat agctgtaata atagagaagc
    31801 ttgattacct gtctctttat tatttctgac cgtattaata tccagatttg tattcctttt
    31861 agtaaataca tcctgaaaag taatttttgc ctcatctgcc ggaacatatt ctaaatagcc
    31921 aatatattga aaggattgtc gtttttggcg atcataaagg tcataataac ctctttcacc
    31981 attttctgct tggggaagta gcacaaagag gtcatccgtt ctctggttat aggataaatt
    32041 attattaaag ctaaattgtt ctgacagatg atttagatag gcagataagc taccaatttg
    32101 ataatattcc cagtccagtt ttttcctttc tatatcagca ccaaaaccag tggttaactg
    32161 ccagaattca gctccaccac ttactttgcc agagataaga tagccgtcgc tatttagttt
    32221 attccccaaa acatagcggt ctttttccga acgcagataa cctgcagctc caactgatat
    32281 gagtaaacta tcatctggaa caagatcaag ggaatatcca agcattcctg tctgactatt
    32341 gtaaggctgg agattatcat ctaaatcgct ggaatcataa aagtattcat acccagaaag
    32401 gatagaatgt cttaaaagat tttcgcctga ggataaattg agattcaaaa cagaatgccg
    32461 attatgctgc gaaaagttca tcctttcttc ctgaatatca agagcattta taaacattaa
    32521 ccaggaaggt gacggattca attgataata gagattattt tcataacttt gcacagtctg
    32581 gctggtaaaa aagtaaaggt caaggttttg attctgtgca ccgagactaa taaaaaggct
    32641 aaagataacg actaagagtg ataaattcag cttcactgag ggtttcacct ctacgattta
    32701 aatcaatgcc agtttcttct tgaatctgag ccaaatggtc tcggtcagtt agatgcagaa
    32761 gattattacg taaggttttt ctccggtgtg caaaagcggt ttccagcacc gttaaatatt
    32821 tgtccaaatc aggtatctct gcaggtttgg ctcttggtct caaagaaact accgcggaat
    32881 tgactttagg gataggttca aagtattctc gtggtacttt gataaggata gaagaatcaa
    32941 atattctctg caaacgaaga gtcatcaaac cgtaagcttt agttccagga atagcacaaa
    33001 ttctttcagc aacttcttct tgaagcatta aagtgataga actaaagtag gaatgatatt
    33061 cttccagctt ttctaaaagg ggtgaagtga tattataagg aatattagct actaatttaa
    33121 cgggagtaga ttcctttttt agctccaatt cccaatctat ttgtaaaaca tctaccatct
    33181 tgagttcaat tttatcacca aactcatttt taagcaaggg ttctaatcta tcatccagtt
    33241 caaaagcagt aagtttgcat ccttttgcca ataaagcttg agttaatatt cctttacctg
    33301 gacctatttc ccagactcta tcctcagaac taatctcagc caattttacc atttcttcag
    33361 ctatagcagg ttctttcagg aaatgctgac ccagcgcttt agtaggtctc aagttattgt
    33421 cccttacgga aaattaatct gtctctaaag acagtctgaa gaaagttaga tatttattca
    33481 ggcactaagt ttttgtgctg cagattgttt gcagtttttg caaactttat gagtttgttc
    33541 tccaacttta acttcgtaaa gcaatttaac cccagtacga tgacatactg gacattctcc
    33601 tcttccatga ttaggtaggt ttttaagacc ttttccacga tgattttttg ccattatcta
    33661 agctccttat tattttatga ttttttatta ctaattataa acaataatta taagtatggt
    33721 tattgcgtca attctaatct ataagaaaag ggggtattac ctaccccctt cttttctgaa
    33781 gcgattcatc tattaatgag ataatttatc cgctatatct tttcaaatta caagtctggc
    33841 tatagcgttc cccaggacga agatattctg cagtttcagg cataaattct atcaatgtat
    33901 aatcggggtc atcataacca cgaaaatatt catcaaagaa aaaacactgt tctgccgctt
    33961 ctgcttttag agctggattt tttacaatat gagcaacacc cgttaaacga atatagcctg
    34021 tattacctga ttcatgcaaa ggaacacaca cctcaaccca tttattattt gctatctgat
    34081 taactttagc atctccagtg aaggtaacaa agaaaaagcg gtcatcaaaa acaaacataa
    34141 ccataggcct aacatgaggt tgccttttat ctactgtcgc cagatacaca tactggttct
    34201 gtctaatcca ggaaagtatc tcttgttgta aggcagattc tgaggtgcgt gacatagctc
    34261 aatttcaggg aattattatc ttattaataa aagcttgatt accttctaag ggattatcat
    34321 tgccatcccg gggaaggttt tctatcttgt gaacaacatc cataccattt actactttac
    34381 caaagacagt atgcctccca tttaaccaag gagtgccatc tttcttagtc acaataaaga
    34441 actgagaacc attagtatta ggtccagcat ttgccataca gatataagaa tagaggaccg
    34501 gagacttcaa aacctgttta tgaattggag tactaattcc cgtcatcgtt tgataccatt
    34561 ctatagtttt accatacata ggttctggac tttgtttttc aactacttcc tgggcaatat
    34621 ccattagctc tttaaccgga ttttcttctc tctgcatata aggcacaatc aaatggtccc
    34681 agaccaacat tgctgtgtct tcatcattga ccattccttt aatttcctca cctataatat
    34741 agcattcatc ttcaaattga taaccgggac ctcccattcc atcattagaa cggtccttat
    34801 ctttagtatt gggatcgcca ccttgaatca taaaatcagg aattacacga tgaaaataag
    34861 tgccatcata aaaaccttcc ttgctcaatt taacaaagtt atccactgtt ttaggtgcca
    34921 tatctggcca gagttccagc tcaatattac cataattggt ctctataata gcggttattt
    34981 ttttggcggt aaggtctttt tgccctttta acgcatccga ttcggaggat tcagttttgc
    35041 tggcattaca attaaccagg actaaactaa aagccagaac caacataatc atagcactga
    35101 taccaggaaa ttgtttcatt ttcctatctc ctttatttta tattgcctaa tttcaaccat
    35161 cccatctatg atttccacat aggtgtgatg acgagtccaa tcacctgtat ttatgtattt
    35221 accatttcct atctcttgta tctcaggttg atgactatgt cccatacaca cataatcata
    35281 tttttcttta gtaataatat actttgcata ttttttcaaa ccaacagttt ttttaggttg
    35341 cataaaattt ttcattctgc tggaacgagt taatctcgct gctatagcca gagaaaaatc
    35401 tggatgaaac caggaaaata ttttctttat tccgccaaat ctaattaaac gacggaataa
    35461 cttatagcgg acatcatatt caggatgtaa atctccatgg gtaaagagta ttttcttccc
    35521 atcggcaata aggttgtagc tttcgtcata gatgggtata ggcagatatt tctgaaaaaa
    35581 atcgttaaac cagaaatcat gattaccact aatcattact aattgacagc cttcatcttg
    35641 aatagcagcg agacgatgta ataggggaaa gtattgtttt ataatggtat atttccaatc
    35701 aaaccagagg tcaaaaatat caccgttcag caccagaata tcataatttc cccttgcttc
    35761 agttaaaaaa gagagcacca gttctgcatt ttctttatct ctttcatcaa gcatttgata
    35821 gggatagtgc atatcggaaa caacgcagat tcgcatttta gcctctacag aaaagataat
    35881 agatgatatt acaaccaaat tttaaggctg tttctcgcac ttcttccggg tcattatgag
    35941 tgtaaggatc tgcccagcca tcgctaatat tagtttcata agtatacaga cacattaacc
    36001 ttccattatc atcaaagatg ccaaaagctt gaggaggttt accatcgtgt tcgtgaatct
    36061 tgggtaagcc agagctgaag tcatagaaac aggtaaaaag tgggaatctg ctatccagtt
    36121 caaccagctc atactctgga aatactttct ttatttctct tctaaaagcg gtatccattc
    36181 cataatcatc atcagcatag agaaaaccac ctctcagcat ccagtttctt aagttttgca
    36241 cttcttgctc ggaccagcga atatttccgt gaccagtcat atatacaaag ggataatcat
    36301 ataatttagg gtcactggct ttaaccacac tctgttctat aggaaaattg gtatttaaaa
    36361 ctgaatttac atatctggca agattaggca ggacttccgg gtcattatac caatctccac
    36421 ctccatcata ttgtagacgg gcaaaaccta cttgagaagc attgcctata acagcagata
    36481 gcagggcaaa aagtgaaaga ataatgagaa taagtatata ttttttcatt aattcactac
    36541 atttaattta atacagacca atttattccc tacaatagca tagaaacagt tattgtaatc
    36601 atcataatcg gataaataga gcaatttttc tttaagctga agctcactca taaccaagcc
    36661 ttgttgacta tccagcacac tgatacctct ttctaaagga accacagtta tactacggtc
    36721 tattcttcta tcctttttcg gagttataac gataagatca aggcggggac tactttccac
    36781 attagcagct aaagttaatg tccatttaag atttcctttc tcatctatgg ctatcatctt
    36841 attgttggat agatagagtt ccaatccgaa aggggtggga tggattgaaa gtatggaatc
    36901 ctctaaagct ttttgccaga gcggggagcg agtctttaat tgatagacat agaggttatt
    36961 ggcagatacc agataaagct tctgctggtc aaaagcaggg ggtgcggtta tttcataggg
    37021 cagttcagca ttccaatcgg gttgtaattc aatactacgg gctggagcat ttaccacttt
    37081 ccccaaattt tccagaataa ccgtgctggc ttttatctga gttgtttcta cttctttttc
    37141 cgtgcgatgc aacattacat caattatcct gttaccaaat atcatataaa taatagctaa
    37201 aacggtcccc aaaagaagca agagagtggt tatttccaat cgggttaact ttcttttagg
    37261 cggtttatgt aaaagcggtg tgtttttaag tatgagccac atagcaccag gacttgcact
    37321 atgagtttca attagcggga taatactttg cacatcggtc ttttcattga aaatctggct
    37381 ggcaatattg ccgggtaaca cgataaagtt ttcattttca gggatatgac atttataagt
    37441 tttgactttg atatcacctt cagaccagcc cagaagattg agctcattta aagattggat
    37501 atgataattt ttccagttct ttcctatggt ctcaagtttt ttacctttag ttagagcgca
    37561 aaatactctc ccaaattgaa cgaaatacaa atcgcagtca aaaaggacag caaagaagag
    37621 tgaaatccct ttttcccgta agtctgtttt aaggaatcta ccgtgtagct tccaatgtaa
    37681 ttcagaaaag aaatgctgca tccaattttc tacttccagt ttgcttatat ccagcacatt
    37741 agcattcagt atttccagtt ttatttctgc ttgaatttct tccagaacca cattattttc
    37801 ttgatgagta gtgcagagaa accagccata gcgtccatct ttggaagcgc ggtattcaca
    37861 catactctga ggataagaat caaatttact ttgtatagtc agcatattaa ctccaccatt
    37921 taataaaact acgccagaaa acgcggaata aagttggtat attcatcaac atattggtat
    37981 aggcagcatt agcaagagga cagtagcatt cctttttatg gatagacctg cgttcaagtt
    38041 ctgcttcagg actgaaccaa atttcccgaa aattgtaatt atgtttcctg agattaccga
    38101 gagatttggc tttaatacaa caagtccaga tttcaccatc aggagatatc tgcgctgaag
    38161 caactccaga ataacaggga ataacttgct ttttatccct caaaatcttt ttcaccagat
    38221 tataatactc aatcctaaaa gccatcgtga tttttgccat acccttatat ttctcattct
    38281 ttatccggtg aataagataa tcaattgcgg atttataggc aaccaacgag ggagttatat
    38341 ccgagccaat agtgtccagt tcaactctct cttctgctat ttccgttata taagaatcag
    38401 gattcaattg catcagtcca ctggcaatag caggaaaatt atccacatta aactttgata
    38461 taacagtatg aatacccacc tgcaaattag gaagattcaa agcctttaat ttatgaaaag
    38521 ttgccactgc tttctgataa ttacctggca cattacgtat agcatcgtgt tgttcttcca
    38581 atccatcaat agagatattt accaccagct gtgctttagg gcaagcttta gcgatagccg
    38641 caactttatc tactatcaca ttggtcaaga taccattagt gggaatattc attatatgag
    38701 gacgagagat attgtaaatc gtagtaacta cctccacaat atcactacgt aagaaaggtt
    38761 cacctccact gaaagttatc cagtaaggtg ctttaccaat tttctgaaag gtctttttat
    38821 attcttcaac ggtaaagttt ttggctttct ttttccagat attacaggtg ctacagcgag
    38881 aattgcaggt atagagcaag ctaacggtat aattgatagg caggacacga ggaaaaccaa
    38941 gatgcttcat cagccagcag cctatcatct gaaacattaa ttttatcatt attctaaaat
    39001 ctacctcaat ggtgaagatt ttttgtggaa cgagcgatgt cccaggcaaa aggatttttc
    39061 tttttcacat aaaaatcaaa actaccgagt aagcgacaaa aagcttccag tgccattaca
    39121 gctatgactt taaaagcagt ggaaggatat tctcttattt cttttaggac aactttaaca
    39181 agagttccca tatcctgcga ggaaacctca tagttttgtt tcttttttag ccagagatga
    39241 ccattctgga tgcgtcttct ttgttttatg aaatctttaa gattgagtgg acctttattt
    39301 ttgataattg catccgggat atactttagt ttatatccct gggattgaac tatagcttct
    39361 atgctggctt catctacagc tgaatctgaa ggaatagcat ccatcacttt acggaaggca
    39421 atcatttccc ctaacttagg cgaaattagt gccatccgat ggtgcaatct ccaaagtaag
    39481 tggacggcat atcccataaa acttttatca tcatttacgg gaatcggtct gccacctgtt
    39541 gctccaatag ttgggtcttc aaaagtagaa accagttttt ctatagtggt gggagcagga
    39601 ataacatctc cagaaattac caccaaaata tcagattttg cctcttttat aaatagattg
    39661 atagcactgg atttaccttc tcttttagcc tgacgaatca gccgaacctg aggatgggtc
    39721 tctgcaaatt gagtaaccaa ttcatccgtg ccatccgtgc taccactgga aactacaatt
    39781 atttcttcaa tttctacttt ctccagtttc tgattgacca gtgcctcaag taaaggtatt
    39841 atattttctg cttcattata agcaaaaaca cctaaggagc atttaatttt actattgcta
    39901 tccataaaac ttagagtttg ctgccctcta ttttctctct ttcctgtaaa tctttattta
    39961 ctgcatccaa aacaccattt aagaattttc ctgtcgctct atcacaaaat tttttggcta
    40021 tttccagagc ttcatttatg atgaccggag gaggtgtatc agtaaacatc aattcatata
    40081 cagcaatata aagaatgctc ctatccatag tggacattct tgaaatcgtc cagttttcac
    40141 tgtgcttttc tatctctttt tcaatatctt tctgatgtat gatatagttc ataaccaaat
    40201 cattagcaaa agcaaaaacc tcagaataat tcgctaaacc atccacttcg gctaattctt
    40261 ctaaaatttc gggatataca tttaacattt cgtgttctat gtattcagga ttaaattccc
    40321 gaaaagatag cgcataaaga gtctgagcag cgagttctcg agctttacgt ctctgtccca
    40381 attttcaatt ccgaaagcag attgagcatt tccaatgccg cactggcagc ataatagcct
    40441 ttattacctg actttgctcc agctctttcc agagcttgtt ccagagtgtc agtagtcaaa
    40501 acaccaaaga ttacaggctt atcttgcttt aaagccagct gggcaattcc cttactgact
    40561 tctgaagcaa tgtattcaaa atgaggagta gaaccacgaa tcacggcacc taaacagatt
    40621 acggcatcta tatcccggcg ttgaactgcc acttttgcca cctgaggtat ttcaaatgcc
    40681 ccaggtaccc aaataatatc aatatcctct tcttttacat tatgcctaag aagaaaatct
    40741 attgctccat caaccaattt ttgactcact atttcattaa aacgactgac caccagagca
    40801 aaccgatact ctctggaaat cagatttccc tgtataactt tcatatgata acctcctgtg
    40861 agtaagctaa ttatttatta ttaaccattt tattacttta gcttattctt tcaagtaaaa
    40921 tctttaacct ctgattttag ctatccgtca tatatatatt tcttattggt aatattttca
    40981 ttgattttca ccctactccc tcctggctct ctcctgcctc ttttatgctt aaagagctaa
    41041 gagagaggta agtgcgagac aagagattat atatatctta ttatgcagta agataggact
    41101 atttaatgtc tatttaaact aaggataagt ggcttttttc gcttagttac gcaaataaat
    41161 gttgatagga ttaattattt cttcggcata gatataataa ggtttaatct atcaaaagat
    41221 agaaattgat tgacggaata tgagtttgaa aaaataagag ataaatctgt aaatggggag
    41281 acaatgaaca gactaataga gcctgaatta gtaaagatag tcaatagttt tgattcaaaa
    41341 aacgattgtt tagcttatat ggttggttta ttagcagaaa gtggatgctt gaataaacca
    41401 gacagatttt tggcagcagt taaaggacgc gaagagatta tgagtacagg tataggaaga
    41461 ggcgttgcca ttcctcatgc tcgtgatttg acggttactg ctctgaagat ggctgtttgt
    41521 gttatacatg agcccttaga ttttatgagt gtggataact tacctgtcca attagtcttt
    41581 atgatagctg ttccccagga tttgaacaag gagtatatga aaatattacg cgtcttagct
    41641 gaatatttac gtgatgacaa taatcgtact accttaatca acgccagaga tgaaaaggaa
    41701 ttatataatg aagtgcaaca cattgaagaa actatggtta atgctcttac tacttagttt
    41761 gagtttcatc cctgtttttg ccgtaaacga agaagcagga actacaggtt ttgccaattt
    41821 aaagaatgtg tactcggctc gtgctattgc tctgggtcaa tctttgactg gacaggtacg
    41881 caatcccgac ggtctatatt tcaatcctgc ggctatttta aatatagaag gtaaggaatt
    41941 gggtactacc tacactaatt cctttataga cactcaagga ggacaaatcc agtatctctt
    42001 gccaaaaaac aaatttactg cttggggatt tgctattaaa tatatgaata tgggttctat
    42061 ggagcgcact gaagttgaca acaatggaga tttaatatct acaggtgaga cttttggtgc
    42121 ttatgatata ataggaagtg tttctctggc aaaatatatc accgatgctg tagatttagg
    42181 tggcactctg aaagtgattt atgaccagat agatgataaa tctgcctctg cggttatggt
    42241 ggatttaggg atgatacacc atccggctaa tcctaatgtg aaagtgggaa ttagcttgcg
    42301 taatatagga tttcagacct cttactattc tgccacagat tataaagaga agctaccttt
    42361 cacttttgct ggaggaatca cctatcagtt tatccccact ttactcggat gtttggaaat
    42421 agataaggtt acaggagaaa atatattagc taaattaggt ctggaatatg aactcaatcc
    42481 ggcacttgct ctccgagctg gattccgtag taatgcttcg gaaggttata atggcggtaa
    42541 tttagcattt ctttctggat tttccttagg agcaggatgg aattggaaaa attgtcgtct
    42601 ggactatggt gttacttcct atggagattt aggtttaata aatcagctca gcttgagcta
    42661 tgaattttaa ttatccaatt tagtgactaa taccatatct tatgtctgac ttccttctgg
    42721 aacttggatt tgaggaaata ccaccctctc agttacaacc tgtggtggaa tatatccaga
    42781 attccttcct caatctaatg aaaagcaccg cactgagtta ttctgcttta aaagttagct
    42841 ctacacctcg tcgtttcttc ctcttagctt catccgttca agagaaacag gaagaccttc
    42901 aggtaaaaaa aatcggacca gcgaaaaaag ttgcctttga tgaaaaaggt aatctgaccg
    42961 ctgctgctct cggctttctc aagaaaaacc attccaaacc agaggattta tatattgaga
    43021 ctacagataa aggtgaattt atcgctttga attatgttca aatgggaaaa gctactcctg
    43081 atattttaaa agactggatt tatgagctta tcccccacct tccttttact aaaactatga
    43141 tttggaatga atccagaatg gctctggcac gtcctcttcg ctggctatgt attctctgga
    43201 atgaagaaat tattccgttg gaaattgccg gagtaaaaag tggtaatatc acctttggta
    43261 ataggtatct tggtttaaat agacctgtaa aaatagattc tccttcagta tatctttcta
    43321 ccttgcaaga aaatgccgtt ctggcagaaa gagcatatag aaaggaaaaa ataattgaac
    43381 aattggatgg tctccctctg gaaaatggac ttcaaatcat tcctgataaa caattaattg
    43441 aaactgttac tgacctggtg gaatatccga ctgcggtatc agcaagtttc caggaaaagt
    43501 acctatttct gccggataaa attattacct ccactatcag tcaaaatcag aaatgctttt
    43561 cggttcagac taaagatgga aaactaagca ataaatttat ctttatctcc aatggcaatc
    43621 cagatttctc cgaggttatt cgtaagggaa atgaaaaggt ggtggatgca agattggctg
    43681 atgcactctg gtattttcag gaagatacta aacaacctct ggaagctttt gttcctcaat
    43741 taaaagaagt gatattccaa tcccgtctgg gaacaatggc agataagact caaagaatag
    43801 agaaaataac tgaatatatc tgtcttcttt tacatcttga cacgcaacaa aaagagcttg
    43861 ctcttcgtac agctttgctt tgtaaggctg acctggtaac taatatgctt ggagaaaaag
    43921 aattcacaaa acttcaaggt tatatcggaa aacaatatgc tttagctggg ggtgaacctt
    43981 atgaagttgc tgaaggaatt tatgaacact atatgccacg aggtccaaat gattctcttc
    44041 ctcaaaccct ttctggggca atagtagcag tagcagataa aatggatagt gtttgtggaa
    44101 taattggaat aggcttgata ccaacaggtt ctgctgaccc ttttgcaatc agaagagctg
    44161 ccaatggagt tgtccagatt atagcagacc gcgaatggca tttcaatctt tttagcttaa
    44221 tccattatac cttagaatta ttaaaaaact atactcagct cactgccact gctgagcaag
    44281 atgtgcaaga tttcttccat caaagagtgg aatggctatt aaaacaatta aaactggact
    44341 atgatgttat agatagctta gaacatctgg ctctgaacga tattcccgat ttaatacagc
    44401 gtgctaaagc tctgcaagca tatcgtgctg aagaagattt tatccgattg gttattggat
    44461 ttaaacgtgt ctctaatatc attaatggag aaaaagattt ccttccttta aaagaagacc
    44521 tctttcagca ggaagaagaa aagattctat attccgaatt gctaaaactg cgtgataaaa
    44581 ttgacctttg cttaagagaa tcagattata aaggtgctat ccgattactt atagactatg
    44641 gagtcttcat tgataatttc tttgatgccg tgttagtcaa ttgcgatgaa ccagaactta
    44701 gagctaaccg ttatgcctta ttaaaagaga taaagaatga atttctccgt attgctgata
    44761 tatctcgtct ggtggtagaa acaaataaaa atgagaatta agataatctt atctggaaag
    44821 tatctaagag attttttaat aaataaatat agaggtaaat aaaatgtccc tcaagatttc
    44881 caaccgctta aaattggtac aaccttcacc tacactaact atttctaata aagccaatga
    44941 aatgattgct caaggaattg atgtggttaa ttttggagtg ggagaaccgg attttaatac
    45001 tccggaatat atcaaaagag cggctattga tgctattgaa gccaatttta cccgctatac
    45061 tgccaatgct ggaattccag aattacgccg tgctatttgt aataagcttt tgcgggataa
    45121 tcatcttaaa tatgagccca aacagattct ggtttctcca ggggctaaag ctgctattct
    45181 gaatgtcttg attgctgtat gcgatgttca ggaccaggtc ttaatgcctg ctccctactg
    45241 ggttagttat ccttatcaag caatactggc aaatgccgaa cccgtaatca ttcctacttc
    45301 tgaggaaaat aattataaac ttactgctct ggcattggaa gaagcaataa agactcatcc
    45361 ctgctctaaa gtgcttttac taaatagccc caataatcca accggtgctg tctatactaa
    45421 aaaagaatta gaagaaatag ctgaaatttg tctgaaatat aagattctgg tcatttcaga
    45481 tgaaatctat gaacgattag tttacgatga cactgaacat atatctatag cctctatcag
    45541 tccagaaatg caagaacaga caatagtaat caatggagtt tccaaagcct atgctatgac
    45601 tggttggcgc ttaggttatg ccgctggtcc ttctaatata atttccgctg caggaagagt
    45661 tcaggaacat gccacttctt gtgtcaattc aattacacaa aaagcctgcg tggtagcttt
    45721 gaccgaagaa gatgattcca tagaaaatat gcgcaaggaa tttgaaaaga gacgtaattt
    45781 tctctttact gaactcaata aattaccgca tataaattgt ttcaagcctc aaggtgcttt
    45841 ttatataatg cctaatattg gatggtatct tgagcataat aaacagtaca taaatggttg
    45901 tgaccaattc tgtgagattc tgttggaaaa gcattatgta gctctggtat ctggaacatc
    45961 ttttggactt gacaccaatg tgcgtttttc ttatgcgaat agtatggaaa atttggaaaa
    46021 gggagtgaaa agatttggag cttttctgga agaattgaaa ccttaatacc ttaataaagg
    46081 aacaacgatg ttaaaaaatg aagaaaaacc tcattttgat tggcgtactc ctcgtaagaa
    46141 gaattatgat tggaaacgct taatcattat ggtagttctt ctggtggtaa ttttgatagc
    46201 catagaccgt ttgaataaga taagcaaagt tactgaacaa cctgctgcag aatttataga
    46261 atccgatacc ctcaattctg gaattaatcc tctgccccac ccatcaaaat gagtctggtt
    46321 gtagtcggtt ccattgcttt agatagcata gagactccta agggaaaaat agaaaattcc
    46381 ctcggaggtt cagcaatata tgcctctctt gctgcttcct attttggtcc agcttatgta
    46441 gttggagtag tgggagaaga ttttcccgaa gaagcaattc gtttgctgaa agaacataat
    46501 attaatcttg aagggttaga aactaaaatg ggcaaaactt tccgctggaa aggagtttat
    46561 aacaactgga atcaagctga gactttgaat accgaattaa atgtttttgc cgatttctct
    46621 ccttcacttc ccgattccta tagaaaatgt aatagccttc ttttagccaa catacatcca
    46681 gaattgcaag ggaaagttct aaaacaaatg aaaagttacc gctgggtcgc ttgtgacaca
    46741 atgaattact ggattaacct ttgtccagaa gagctaacga aggttatcca taaagtagat
    46801 atccttttca tcaacgaaga tgaaattagg caatacaccg gtattgataa tatcttcaaa
    46861 gctgcccgaa tgatactgaa atctaaagtt caagtggtag tggtaaaaag aggtgaatat
    46921 ggtagtgtgg caattttgcc tgatgacctt ttcttttcac ctgcctatcc actggaagag
    46981 atttttgatc ccacaggagc aggagatagt tttgctggag cttttatgag ttatctggct
    47041 tctcaaagct cgttggataa aaataccatc cattccgcta ttcgctatgg aacagtgctg
    47101 gcagctaaat gcgtctctga ttttggtgtg cgtggcttga ttcatcagga tttttccgat
    47161 ttacaaaaga tggttgatca actggaaagt tggacttaag atattatgaa taataataaa
    47221 cttgattatc gtagttctgg agttgatatc aaagcaggag aaaaagcagt agaggcaatc
    47281 cgcgaaatgg ttaagggcac ctataatcct aatgtattaa gcgaattagg cagttttggg
    47341 ggattatata gatttgatag ttcttcttat actgaaccaa tactcgtttc cagcactgat
    47401 ggtgtaggaa ccaaaatacg tattgctata gaggcacaaa aatgggatac cgttggacaa
    47461 gatttagtaa atcattgtgt taatgatatt cttgttcagg gagcggaacc tctattcttt
    47521 cttgattata tcggcttagg gaaaatgaat cctgataaag tgcgcattat tatctctgga
    47581 atggttaaag cttgtaaaga aaatggctgt gctctgattg gaggagaaat ggcagaaatg
    47641 ccaggattct atcaggaaga agattttgac ttagtgggta ctattgtagg agtggttgac
    47701 cgtcctcgtt tgctccccag gaatattcaa aaaaatgatt tgataattgg tataccaagc
    47761 tcaggattac acaccaatgg ctattccctg gtgcgaaaaa taatctttga aaagttgaaa
    47821 cttaatattg atagttatat ccctgagctc aattctaccg tggaagaagc cttactttcc
    47881 atacataaaa gttatcttcc cctacttcgt cctttcttat ctatacccta tcttcatggt
    47941 cttgctcata ttactggtgg tggcattcct ggtaatctta aaagaattat tcctgatgga
    48001 ttgacggctt atctaaacta tgaaagcaag aatatgcctc ctttgtttca ctggctgcaa
    48061 gactctggaa atcttagttg ggaagatatg ctggaaactt ttaatctggg aattggtatg
    48121 atagcagtgg tagaagataa atacagtgat gaatttataa ctgctactgc ttccaaagtt
    48181 ttaggctata tagatgaaag tgcagataag aaagaaaaag taatagtcag ttataaataa
    48241 aaattactat gtttttaatg tatgataaat aaagaaaata atgcattgaa tatagcttta
    48301 gttattatag aatttagtaa atgaggttat gaacattgaa aaaattcttg acgattttag
    48361 cttataacca aaatagatat ttacacttca aatataaaaa tgaagttgcg ttttttacat
    48421 agacaataat ggtttataaa aagggaggtt catcgatgcg aaaacctgca ttgatcctgc
    48481 tgattttact gattggctgt ataagtttat taaatgcagc cacgactgga cgtttagcag
    48541 tccgagttcg tgacaataat aataaacctt tagaatttgt taatatcgtt gttatgcgag
    48601 gcaatcaacg tataacagga ggacagaccg attctaaggg tgtggcaata attattaata
    48661 ttccccctgg tgtatatacg gttaaacttt ccctgattgg ttatgacccc atcacttata
    48721 ccgatgtacg aattgaagtt gatgagacca ctaatatcaa tgctattatg aaaaaatcag
    48781 gggttaaatt agcaccagta gttgttactg caaaccctga tgtagttggt aaaagtcaga
    48841 taggttcggt ccgaaaaata gaaatggaaa gaatgagtga agttgcagca gctgatctta
    48901 ctgatatagt ctcacttcag gctggagtct ccaatatcgg tggtgaatta catattcgtg
    48961 gtggaagatc caatgaaata aatttcaccg tggacggtat gtctgtttcc gatccagttg
    49021 atggaggaag tgctttacag gttgatacag atgctattgc cgatatgaag gtgatgactg
    49081 ggggattccc tgctgaatat ggaaatgctc aatccggagt tataaatata gttaccaaag
    49141 acggagaccc attctattca ggaaaagttg aatataatac tgaccatctc attggtgaag
    49201 gaagaaactc cgatgtaaca aagtttgctc tcggaggtcc agtttggcct cttggtggac
    49261 aggatttaag agaaagattc accttttatt tgaatggagg aggagaatgg ttagatggga
    49321 gattgaaaga ttattatatt gctgatccta tgaaagattt tacctttaat ggaatcccca
    49381 tcctggaata tgagtatcca ccttctaacc cttatgctga ccgaaaagat ttcttaggta
    49441 tagatctggg aaatagaaat tataattcat ataatataaa tttgaaatct aaatatgtgt
    49501 ttaatcctgc acaaaaagtt acctttgccg ttcgtggtga tcgttctttg aactatcctt
    49561 tttcttacgc ttggcgttat gctttagacc actttgcgta tgacgaaact attcagagac
    49621 agtatattgc aacatatgac catgtcttca attcggcaat gaatctgtcc gttaaagcca
    49681 gttattatca aaaagatagt aattcgggcc ctcgtggaat tcagcgcaat aattacattt
    49741 atatgtataa taatttagat cccaataatc caggaccgga ctatgtggat aatgtattga
    49801 tgggtattga aggatgggat actgttgacc atgataatga tggtgtttat agcccagatt
    49861 ataatggcga tggaatcagt gatgccgatt accttcctgc tgccttctgg gcttatcgta
    49921 tagcaggttt agaagatcca cgctctattc caggcttttt accaccagga actatctatc
    49981 aatattttgt ggatgatgtc acaactattc tcagtggtag agctgatttt gagtggcaat
    50041 taaatgaaac tcatttggct aaaacaggat tggaattaat aaaacataat attaaaaaga
    50101 accagttaca gaactttctt aatatctatc aagatcgtta tcaagccagt ctaaaacggg
    50161 tttatgatat ggcaaatctt caatggactc ccccttcgga cgctcctggt gataccactt
    50221 attatgggat tgtaccagaa ttgtatgcca tatatgtgtc tgaagataat gttttaattc
    50281 ctatctataa ccctaatgat tattataaag cagctcgtga agcttctggt aaaagagatg
    50341 gttatcaagc tttcccctgg caagcagctt attatatcca ggataaaatg gaatgggaag
    50401 gaatgattgt taatgctggt ctgcgttttg acctctggta tttaggttct aaatacaaag
    50461 tcttgcagga tgatggttct tatcgtacgg ttgaatttga tcccgatgaa agattacaag
    50521 ttatggtatc tcccagatta ggtgtctctc atcctataac tgatagagat gtgatgcgtt
    50581 ttgcatacaa ttatcagaat cagcttcccc agatgcaata tatatttact tctaaaactc
    50641 catctgatgc ctatatttct gaccagataa taaccgttgg taacactaaa ttggaacccc
    50701 agattactgt tacctatgaa gtaggtttat cccaccagct ttctgatgat tatgtggttg
    50761 atatgactgc ctattataag aatatatata attatgttag cactattaag gaatatgacc
    50821 ctaatgaacc tcaagtttac tggtataaat atatatctga ggactatggc tctgctcggg
    50881 gtattgacat acagctggaa aaacttatgt ctaactttac taactggtct atcgcctatt
    50941 ctttagcctg ggcacagggt aataattcta ccaccgtaat ccaagatgaa gccactaatc
    51001 ttagagaatt ccctctggat tgggatatac gccataatat tggcatcaat tttaccttta
    51061 gaattggtcg gggagaagaa ttctttattc ccttcactaa ttatatttta cctttagacg
    51121 atttcaccgc caattttaat tggacgtttg cctctggtgc accttacaca cctcaatcat
    51181 taataggtga taagctgctg gatacgaata gtgctcgcaa agaccccact catcagctga
    51241 atatgagatt gacaaaagga tttatgcttt ctaataagat gaatattagg gtctttttag
    51301 atgtggaaaa tcttctcaaa accaaaaatg tgcttactgt ctatcccaaa actggtttat
    51361 ggtatgatga tggagcagac ctggcagata gcaatactgg ttatgtctat ccagaggtta
    51421 aattcgttca tgatttatat acacgcaacc ccggatttgt taatgatttc cgaggtgtaa
    51481 cacttggtat ctcattcaac ttttagtaat atccatggag gaaatataat aaaatgagaa
    51541 acagaatatc aaaaatacta ccacttctgc ttctaatgag tgtcttactc atttctttag
    51601 cctatgccca agataatgcg actgctacag ctccagatac agccgttact acaacgacaa
    51661 ccacagaaca gacatctcca actgaaactg aagcggttca aaagtcatcc ggtctaagat
    51721 cggtagctga atttctgttt ggacgtgctt tagtgcagga attcttgaat ggtggttggg
    51781 ctatgtggcc cattcttgcc gtatttattt atggaattgc ctattctatc tggaaattta
    51841 tctctttgat gtatgctaaa atcaatctga atgatttctt aaatcaaatt gtccctctaa
    51901 ttaaagaaaa gaaaatcaag gaagcaattg aaactgcaaa aaagactcgt ggaccagttg
    51961 cagcggtggt ttttgccggt ttagaaaaag ccgaacaagg tattgaagct gtagaaaaaa
    52021 atatggaaaa tgccgctatg attgaaatgt cttatctgga aaagggattt gtggaaatga
    52081 cttctacaat tgcaatctgt cctatgttag gattcttggg aacagttgat ggtatgatta
    52141 ttgcctttga cgctatatct aaagccagag cagtagatgc tactattgtt gcagatggta
    52201 ttaaagtagc tttgattacc acaaagtggg gtttaatttc tgctattcct gtgcagctat
    52261 tatataatat attcaccact atggtggatg gtattgtggt agatatgcaa agagcttcag
    52321 aaaaggttac agaagcccta attgaagcct aaatcgaggt tacaatgaac gtaactcgga
    52381 agataagacc acgcgccagt attcctacga catcaacggg agacatttct ttcctactta
    52441 tagtcttctt tatgtccacc accaaatttg atgttaaaga aggcgttaag gtccaactaa
    52501 ataaagctgt cccggaagaa caagttagaa ccactgagat aaaacttact gaagcagaaa
    52561 tgaccagatt ggaaattcta cctgatggaa gaataaaagt taataatgaa gagcccagag
    52621 ttttcactga tgaggagttg gttaacttga ttcaaagtaa gattgagatg cgagaaagac
    52681 ttaatcagca aagtagtgac cccgaagtga gaaaaacaaa gatgctcttt ctggtaaaaa
    52741 cagatagcga agccaaatat agcgatatgg ttagagtggt agaccatctc gttagcttta
    52801 gagatagggc aatgatttca atttccaccc aattatagga gtatattatg gctaaaatac
    52861 aaaagacaaa aagacctact gcatctatcc caacaacttc tacgggtgat atatctttct
    52921 tgctcctact cttctttatg gtttctactg tttttgtcca ggaacgcgtt ttttgggttc
    52981 cccgtgaaag attacccttc gctactgaaa acatagaacg catagcccga aatgaaactg
    53041 ctaccatcta tattaccctc aagggacaaa ttcttattga tgacttccct tattcagttg
    53101 aagatgaatc tatagttgag actatgcgaa caaaagcacg tgagattccc acccttcagg
    53161 tctgtttccg agctgataaa gactgtcgtt atcaatctat ccgtgatgtt atgattcgcc
    53221 ttcaagacgc agatacccgt aatgtggtat ttgaagtcca aaggaagtta taggaagggg
    53281 gaattatgga aaacaataaa attttcgatt ggaaagacta ctgcgatgct caattcagca
    53341 aagctgttgc cttagctata gcgattatgc tattgattta tctcgttgcc ccaaaaactg
    53401 agactcaaac caaaaaagaa atcatttcac aagtagaaat cgctgaagca ccacctgaca
    53461 ccagacaaaa agaagaaact caaaccgaaa cagaaattga tattgatatc cccgttatca
    53521 gtgaagaact tgccacagaa gatacacctg aattagctga aaagagagcc ttagctttgc
    53581 aacagtttgg aaatctacag agcaccacca gttccacaat tcaacaatca ggtgaagagc
    53641 gaccctttga ttttgtcccc tgggatgatc ctcctgtccc tataggaagt ataagaccgg
    53701 tttatcctga atttgcaaga agagcgggaa ttcagggtac tgttattctt gaagttgatg
    53761 tttataaaga tggaactgtg ggaaacatta gagttgtccg ttccgttcaa cccggtcctg
    53821 gaggtttaga tgaagccgct atagaagctg ttcgtaaagt ccgttttcaa ccgggaaaaa
    53881 gtagcggaaa tcctgtagat acgaccgtta tagtcccaat tgaattcaaa ttgaattaat
    53941 ccacatagga gcttaaaaaa tgaagtttaa taaatttatc ttttgtttgc tgcttgcctt
    54001 attattcatt ggaattgctc aagcagcaat ggtagacaaa ccttctggag ctaagaaact
    54061 tgacatagat cactatcata atgtcggtaa tatgtggttg agagtaagta actatggctt
    54121 ctttggttcc ggtgatgatg ctgtcccgca atatccttct ctggaatacc ctggaggtca
    54181 aggaatagat tatctttatc agggcgctct ttggttcggt gcaaaaaaat accgaagaga
    54241 tagtgctggt agaaaacttt actggcgtgc tctcaatcca agcgccgata gctctcagac
    54301 tgttccttat ggatctcctg attggcaacc ctggatgaaa cctgtaatgg acaccctggt
    54361 ttctgttggt tttgatggtg attgggacat atatgagttc ttacctgctt ataatccttt
    54421 ggtttctgca aacactaatc cggaagtcac agctatgttt ggtatataca acggattaga
    54481 tcgtatgatt acctcaagta ctcgcaccca aaaaagagga gtagatgatg atggtgatgg
    54541 atgtatagat gaggattttg ttggttatac tttccctttg cgaagagcag atgagttacc
    54601 aattcaattc caaccctttg gtggtcagtt tatagcagac actaataatt ataatatcat
    54661 caatactggc aataatatgg agatttggtt ccctttaggt tttatggacc tttccgatac
    54721 atcatttaga agttattgtt tctctatgcc ctatgatgat gataatgacg gacaaattga
    54781 tgaagatggt gctcctgtct cagaacaaga ttttatagga tattactatg attattgccc
    54841 ctttggaacc ccaggtgacc gtgattatgg aggttcacgt ggttcaagta agcattttcc
    54901 acttaatgta cgggtaagac agatgtccta ccaatggagt tatgaataca tcaaaaactt
    54961 ggtgtatgtg gaatttgaca taaccaatat gaatactgtt tatatggata ctctttttga
    55021 ttgtgcaatg ggaatttata tggattgtga ttgcggtcct caggtctggg gtcgtgaaaa
    55081 ggctgaagat gatgtttcag gttatgttaa aggttctggt tatgaatttg cttacactcg
    55141 tgatgctgat ggagatggtg gtctaaccac tggatatgtt ggtgcacgag tctgcactcc
    55201 agacccagaa caacttcaat tccattgctg gtattggaaa gtgggaaatg gtccaaaaga
    55261 tactgatcct cttaatttaa attatgcacc taaaaaaaca gctaatgaaa aatactggtt
    55321 actgactggt agaaaccctg atgaaacgaa atttgaccct ctgcgccctg aacaaataga
    55381 ccagatggag tttgaagaaa cagttcctaa agatacacgt tttctatttg ccttctatgg
    55441 tgatcagaat ggtgttactg ctccaacaga gaattcctgg aatcttgctc ctggaagaac
    55501 tatgaaaatt gttgtagcgg ttttccctgg tgataacttg gaagatttaa agagcactgc
    55561 aaccttcgca aaaatgatct atggacaagc tcaaacttta aatactgtcg tattacctga
    55621 tactttccca cattattctc ctccagaacc tccagatatt ccaaaactat ttgccgaaat
    55681 gactgacgat gggagtcgta ttgaacttta ttgggataac agaagtgagt tttcttatga
    55741 tgttatgact gtaagtagtt cagttatcgg ttggcaagat cccaatagcc catttttaat
    55801 tcctggaatt gatagtgacc ccaattctgt tgattggtct aatttccctg atgaattcaa
    55861 acctaaccca aataattata atatgaatgc acgagtgaat cctttcactg gttacaggct
    55921 aaggcacgat tttcaaggtt atactacctg gggacgaagc ggtagtggtt ctcaagaaga
    55981 ttgggtaatg caagaacgct gggataaaat tgatacccca caagacctgc tggattataa
    56041 tgtgaatatt ggtactgaat attatattaa ttatggtgga tatctgggca ttgacaaagg
    56101 tcttcccaat aagaaccaat ggactggtga tattaccaaa ttctacagat atgatgagac
    56161 ctatcaattc gtaccctatg aaaatggtga tgatttttat ggctggccta tttataaccc
    56221 tgaggtagaa tggagtcttg aattacagca acaggctgca caaattgaag tggattatgc
    56281 agatctacct ccttcaatag ttaaaaatct taaggcaaga ttatttaagc accccgaaat
    56341 tcgggatgat atctttgatg ctatttatga ccctaaaatg attcccttac ctggttttgg
    56401 cggtcaagtt tatataccta ctaatcctaa tgatcaaagc cagtgggaga aattgaccga
    56461 attaaaaaag caacgtcttg cccgtagata ctatcattcc gctattttat atccacccaa
    56521 aggaattgaa tattatatag cagttaccac ttttgaccgt ggtatccctc tcaatcacat
    56581 tagttctctt gaatctggac gtgataaaga tgccaatatg aaagtgtttt tccctggcac
    56641 cgtagcaaaa tctgatatga ataatatcta tgttgtgcca aatccctaca ttggaaagag
    56701 taaatttgac ggaagaagag aaaaggatga aaaaggtgat aaaagtcgcc gtatctggtt
    56761 cgtcaatctt ccggaaagat gcaaaataaa aatatatacc cttgcgggag acttagttga
    56821 tgaaatagac catgatggtt cttatcaagc agatattctt actgtatcta aagctacaac
    56881 tcacggtcta actgccagcg gtatgcatga ttgggactta ctctccaaac ataaccagat
    56941 tattgcacct ggagtttatc tctattccgt agaaaataaa gctgacaata agataaaagt
    57001 cggcaaattc gtgataatca aataaggagg aatggtgaaa aagaatttaa tattcctgat
    57061 tgcattgagt cttatcttaa ttcctaccct gatttctgcc aaaccattcg gaaaagtagg
    57121 aacagtagcc ttacaattcc tcaaattggg tgttgacgca cgtgcgattg gtatgggtga
    57181 agcttatact gctgtaacag acgatatttc ttccgtctat tggaatccag ctggattagc
    57241 tcctgcttat caaaatcaag ctttcgtctc tcataccaat tgggtcgctg atattatgca
    57301 tgaatttgcc gcttgctcat ttaccaatgg tgtttccact gtcgctgctt atggatctgt
    57361 cttgcatatg gatgatatgg atatcaccga tgaagaaaca ttcggtccca ctggtgaaaa
    57421 atttaccaat agtagtatgg ctttcggggt agattatgct caacagttta ctaataagtt
    57481 ctctgctgga gtaggagtta aatacttacg ggaaaacctg tatgagttct ccgtcaatag
    57541 cttctcggtt gatgttggct ctatgtataa cacaggatgg agaaatatta aaataggtat
    57601 ggctctgcgt aatttcggac cggatatcca ttattatgtt gatgatgatg aagatggtgc
    57661 cactgatgag gacccttttg atctctttga taatgatggc gatggggcta ttgatgaaga
    57721 tggtcccgaa ctggattcta agatacctat gtgcttctct ttgggtgtta gcggtgatat
    57781 tatgcgcacc tggaatgaaa aaatccccac cgatgaaaga actatttata attccttaaa
    57841 tcattggatt gtttctattc aattagacaa tgttattgac cgtatggaga catggaatat
    57901 cggcactgaa tataaatacg gtaacctatt tttgcgtgcc ggttatcaaa ttaattatga
    57961 tgcaaatggc ttctctgccg gggtcggtta tcaaatacca accaatttcg ctataattaa
    58021 tgtggattat gcctatactg atatgggcga tttagcagaa agtttcctga aaagtgctca
    58081 tagactttct attaaattta aattctaatt aaacctaata aaatatggag gaaaaaatga
    58141 aaaagacagt agtgattata ttcacacttt gcttgctaat gtccttggct tttgctgatg
    58201 acatggaaat tccaactatt atgaaaccaa taactaactt ccaatatgtt gctaagacag
    58261 ctccccagca ccgggatgct ccttcctata ctttttctaa attaccaact cctataatca
    58321 ccaattacta tgattatatg attggaagtt acaatggatt acccctaaaa gtaatccctc
    58381 aatcagctgg cggtggctat tttatgacct atcatggacg tcgtcaagct ggaggtactc
    58441 gtcgggtttt ttacacttat attgatgccc aagggaacat tcagactaac aacgaaatca
    58501 ctaacttaca aaatcatgaa ggatatccaa caattgaagt tgataaactt caaggaaagc
    58561 caatgtatgc ctggcatgct aacaaagatt cagattctga attagaggta gaattcgctt
    58621 ctgatgcatt cctggatggt attactggcc tttttaatta tgatattatt attaataacc
    58681 ccattactat cactgctcct aatggcacaa ccactacaga taatgaattc atctggccca
    58741 atgctcaaat cggaccttct cctattgcag gaaaacaaag aatttatgta gtagcaagaa
    58801 attcggtcac tcataatact gctaatgccc cttcagaaaa tccttatatt gcttatgcgg
    58861 attttgaacc agatgacatt gtaaatgcta ttcctttggt ttggcactat accagtattc
    58921 ctgaaatgaa tcaatggaat gtggatgttg agtggcgtcg tcctttccat gcaattacag
    58981 ctgataatgc aggaaatctt tattatgcgg gttatcattt tgcaaccgaa tctgatgcat
    59041 caacaaatat agatgaaccg gatttggatg tctttatctg cccaaattat ggtgaaggaa
    59101 cctggagaag agtcagttct tatagccatt tgaatatgtg gaatcccaac tcttctccta
    59161 ctgacacaac cggttatttt gattcacccg ttagcggtct tccttttggt gataatggtg
    59221 aactgtattt tggaattgct tctggtaatt cagggcatat aaatgctact atggataatt
    59281 tgggaagaat ccatgtattt ggtgtctggt ccttacaaaa tattgatgga tattattatc
    59341 ccgaaatgga atttgttaag gaatttatct ttgatccagc tactgaagaa ttctcaataa
    59401 gagaagtata tcctcaaaaa gaccctgagg ataactttaa tactggtttt tgtccctggg
    59461 attaccagcc accttttgga gaagtagatg agtatgttct aacttcctca ggttatgcta
    59521 tttccgtcgc aactggttgg cctttccctc actgggatga ttccgctcat agtgatgcta
    59581 tggaattcca ttataataat tgcaaaatca ccagcgccaa tgagcaaggt atgatggctg
    59641 cagtttggca ggatagcaga cgtgctcgtg attataatta tattggcgac acctcaatgg
    59701 ctgcctatgc taatactccc gaaatctgga tttccatttc ccctgataat ggaaactcct
    59761 ggagcgagcc tatcattatt aacaatgttg atactcccga atttgctgga attaaaccta
    59821 tgtgggttta tcctgccgat aaaattcaat atgtaggtac tgttgatggt cacaaaattg
    59881 gcaaactggg aattatgttc tataatgact acacctgggg tgctaatgtt attgatcctc
    59941 cctatcacca aaatcctgat ggcggacagg ttatgtttat ggaattgctc atcactgcac
    60001 ctgttgctaa tgatgacctt actgttccaa atgtgactta tatgttgcac cagaattatc
    60061 ctaacccctt caatccacaa accactatca gttttgatat gcccaaagct ggatttgcta
    60121 atctctccat ttataatgtg aagggtcagc tggttaaaaa tctggtgaat gataatcttg
    60181 attttggaag acatagcgtc gtctggaatg gaactgacaa caatggcaag cctgttacca
    60241 gtggattata tctctatcgc ttgaccacta atggtgtaac tgaaacaaag aaaatgatgc
    60301 tgatgaaata atccattatc aacatacata aagtattata actaatccgg gtgttttgtt
    60361 tcacccggat tttttattat ctttgctcta tactatgact gactttctaa caagagtagt
    60421 tcaatcaaaa aaaagcttaa ataacattcc ttattgagaa aaggctaaaa ctaaccaaag
    60481 ctatatgcct cctcgtttaa attaaagctt aaaaggatgg gatggctata attataaata
    60541 gatatggttg ctctattctc taagaaaaag ttatctataa gagttaatta gattatcaaa
    60601 gtattgaaga aaaagagaag atagattcta ttttttcaat aacagccaga taaaaaaagg
    60661 acatcccagg gcagaagtga taactcctac aggcaattca ataactgtta tattcttagc
    60721 tataaggtcg cagcacaata aaaatattcc tcccgttatc aaggaataaa gaaatatctt
    60781 cctctgtcct gaaggaataa aaaacctcac tatatgggga atgattaatc ccacaaaacc
    60841 tattatacca gcgtaagaaa cggtgatacc tactaataca gaagttagta aaaagagttc
    60901 tttacgcact ttctgcacat caattcctac acttccagca tagatatctc ctccactcat
    60961 aatatccagt gctgttgaac gaaaataaag tctgcctaaa ataactattg agaccacgag
    61021 taaaaagaga aagaaatgcc attcactctg ggtaaatata tgccccaaat ttcccattaa
    61081 tgtccccaga ataatcattg tatctttctg aaagagatac ataattaaag atataccagc
    61141 agaaaaaaac atccctacaa tcactcctgc aataagtaga cgactacggt caaaactccc
    61201 ttttttctgt gccagacgcc aaacaattat taaagttaaa gctgcaccta taaatccccc
    61261 tacaggcatt aatagtgtca gaccaagcac tccgaataat atcgctccaa aagctgaacc
    61321 cgatgatatt cctaatatat aaggttccgc taatggatta accagcatta attgatagac
    61381 agaacctata ccggcaagag tcattcctgt gaatatcgtt agaatcatac gaggaagtct
    61441 tacttgtatc actatctctt tactaacatt acctataaaa agatagagtg cggctatcgc
    61501 taaaaatata agaatgagtg agattattaa ccagtgttcc tttttcattt tatagtctgc
    61561 tcagaaaata tctcatacaa aagccataaa ccctgaactg tccttggtgt agcttgctga
    61621 attaaatcag ggtctatatc tttttcaaaa taaatccttt ggttctttac tgcaggaata
    61681 ttactccatc ccttgcgtga acatataccc tccagcgtat cctgagaata gcaaataata
    61741 atttctggct gggctaatat cacatcttct gggtctacac gagcataatc ccgttctaat
    61801 cggggaaata tattatcacc acctgcagat tctataacat cacccacaaa agattcatcc
    61861 gaaacactca ttaagggatt gcgatagatt tccacataaa ctctcggatg gtgaatattt
    61921 gctgctctac ttcttacttc ttcaatagct gtagctacac tatctgctaa actctgagct
    61981 tctttttctt tacctattaa atcacctatt tgtttaataa taaccggtaa atcatctaat
    62041 tttttaggat aaatctgctg aacttttatt cctatttttt gcaattcctg ggcaatagca
    62101 tcctgctcca aagcggaagt aaataccagg tctggtttta gagctataac tttctcttta
    62161 ttaattgctc caaaattacc tacaaccggc acctttaata gctcctcagg ataatcgcat
    62221 tccagagttc tacctactaa caggtctgta gctcctaaag aagctatgat ttctgccact
    62281 tctggtgata acactacaat tttttgccca ttgcgcactt gctcttgttt gttacaagaa
    62341 accaatatca tcataagtaa gaacatcaaa caaacgcata catcatattt tcttaacttc
    62401 atcatcatct accataaaaa taaatttgtg cttaagctct ttatcaacac agaataagac
    62461 aaagagcctc tataatttat cctgcttatc aagaataaaa cattcttatc ttctgcgctt
    62521 ccatcactga atctgtcaat attttttcct gctttttcgc atttcttccc tgttccagca
    62581 aagcaaatta ccaatcagga atatattcct acttacataa tgctaaggat ttaagtcttt
    62641 tagataaggc tataccactc attcatataa agccctacgc ttttatacta cctggcaata
    62701 gcttgacaga aaagttaaaa taagatagag gtaatttctt aaaatctaat atgcgaggat
    62761 agtttgcaaa gggttatctt tgaattatat aatatagcca gtataaaggg tcagttaaag
    62821 ctaaaaaaga ctacaaataa attattaacg gatagaaatt atgaacaact ttatcactaa
    62881 ttctaaggaa agcaaaacct taagtgcccg aataagagag cttattagag ccagcgagga
    62941 attgaaattt ttagttggtt tcttttattt ttccgggatt tctgagctta ttgatagttt
    63001 aaaagagaat cctgattgta tcctaaaaat cttggttggt ttaaaaatag atgtgcaaaa
    63061 ttacggaatt attgagtatg gtgattctat aagcaaatct cattctgaaa ttcctgaaga
    63121 atatcttcaa tctgttgtca aagctttgaa taccgatgaa tttgataatg aaatctttta
    63181 cgaacaagtg cgtttcatcc tcagattaat taaggataat agaataatta tcagaaaaac
    63241 tttatatcct aatcattcta aactatacat ctttaatttg aaagaagagc aagtaactcg
    63301 taataaactt tttatcaccg gtagcagtaa cttaaccaga gctggattaa aaagtcgtga
    63361 tgaatttaat gtagaaattt ccgattatgg cgttgaggaa gcagaacaat attatgatga
    63421 tttatggaag agagctgttt ctataaccga agagagtgac ataaaagata aacttattat
    63481 aaatgttgaa gaaaaaaccg gattaaaaag cattcatcct tttgatgcct atatgttcgt
    63541 ggttaaaact tggttagata gtatctataa aagtcaagag gaagattctc tggaagaggt
    63601 actactaaaa aacaattata agccctataa atatcagata gatgccgtca aacaagctca
    63661 atatattatt caagaaaatg gtggtgttat ccttgctgat gtggttggtt taggaaaatc
    63721 aattattgca tgtttaatcg cttaccaatt aaaaggaaga ggtattatta tctgccctcc
    63781 tggattgatt ggtgatgatg ctgcatcttc tggatggaaa aagtataagg aagattttgg
    63841 cttatatggc tgggaaatct tttccggagg agacctggat actgtgctaa aatttattga
    63901 aaataaggat aacattgaaa ctgttattat tgatgaggca catcgctatc gtaatagctc
    63961 tactgaatct tatgatatgc ttaaaactat ttgcagagga agaaaagtca ttcttttaac
    64021 tgctactcct tttaataata ctccagcaga tattctttcc ttactcagtc ttttcattgt
    64081 ccccaagaaa tcaaatataa ctattaccag taatttaagt gatttattcc acttttacaa
    64141 taaccttttt aaagaattag ctttcatcca gaagaattat aagtcaactg accccaaaaa
    64201 tagcggggat gccataaaaa aatatagagc tttatttggc tctgaggaga ttgacatcca
    64261 aaaagtaact gcaagagcaa aattcctgtc tcagcagatt aaagaagttc tggaaccggt
    64321 tactattcgt aggaatagat tggatttaat caatgacccg gattatatgg atgaggttaa
    64381 cgaccttccc agagtggaag accctaaaga atgttatttt caactgacta aagaacaatc
    64441 tgaattttac gatagagtta ttggaagcta ttttaccaat aatcctgata taacagataa
    64501 gttcaaaggt cctatctata agccttataa atatgaaggt aaagataaag aaaaacttac
    64561 cctacaggag caaattgacc aattaagtca agataacttg tatgacatac tgcgtcgtca
    64621 aatggtaaaa cgctttgaaa gttcatttgg tgcttttcaa aaaagcattg aaaacattaa
    64681 aaaatcctat gagacaattc gcactttcat tgaaaaatcc ggcggatcct actttttaaa
    64741 ccgttcttta ataaataaag tcaattatga tgaccctaat agtattgatg agattctaag
    64801 taaatatgaa gatagcatag ttgataaaga cgataaaaaa tacaaggtct acaatgttaa
    64861 tgaattcact ttaaaagatg agttcttcaa tgacattgaa tccgatatta atctcttttc
    64921 caggatatta gccgaaattg atgctctaag acttgtagat aatgatccta aaagacaatc
    64981 tctaattgag gaagtggaaa atatattaca aactcctcct aaaaaaggtg aaccagctcc
    65041 caaagtggta attttttccg aatatgctga tactgttaat tatcttgctc caactttaaa
    65101 atctaaattt gccgaaaaag ttctggtggt tacaggctct ctttccaaca ctcaattaga
    65161 aaaaataaac tccaattttg atgcttccta cagagaagat agacaggaaa atgattatga
    65221 tatcttactc tccactgata aactctctga agggtttaat ctaaaccgtg cttcaaccgt
    65281 tattaattat gatatcccct ggaatcctgt cagagtaatt caaagagtag gtagaattaa
    65341 tagaatcagt aaaaaagtct ttgatactct ctatattata aatttcttcc ctacggaaca
    65401 gggatcggat attgtaagaa gtagactgat tgcagcaagt aaaatgtata tgattcacga
    65461 aaccttagga gaagatgcca gaatatttga tccggaagaa gagccttcac ctgcaaaact
    65521 gtttgcaaaa attcaaacta atcctgataa attagaacca gaaagtcttt acaccaaatt
    65581 gaaaaacaag atgagagaat gggaacagct ttaccctgat agagttaagg ctttagaaga
    65641 tatgcctata agattgaaaa cagctaaacc atataattct gactccttaa atgttttcat
    65701 ccgtaaagga agacttttct gcgtaagcac tgatgccatt aataataatg gtaactatca
    65761 aacgcttcct atggaaaaag ctattgaaag aattgagtgc tctcctgaga ccttgcatct
    65821 tccattaagt aaagatttct ggaataatta taccatttta aaagagaaag caggtgattt
    65881 cttctataat caaaaaggtc gcagcaatgt aaatgaagct ttcaatgtta tttctgacct
    65941 tgcagaagat tccaatccca aacttttacc ctggaaatcc ttccttttta tgttaaaaga
    66001 agatatcgct aattatggca ctttatccga ttacacgctt cggaacattt ctgattggat
    66061 taaaattgga aaagataaag attatgacaa cattatcagt gaattagaac agctaagtca
    66121 aaaattagga aagaattatc tggataaagt taaaaaacaa cttaaatatt atgaccaaga
    66181 agtcatcgta gctattgaga atcaatatgt caaaaaatga atctatcgtt acacctgata
    66241 acttctctca ggaaaaatgg attgattttt tctatagtaa atccaaatat ttcagagaag
    66301 ataacaaact taataaggat tatacagata gcaattttat tgagattaat aaaatcggaa
    66361 agatagaatt tccgcaaagc gttccttctg atttaggtgt ctatgctctt aaggttgcta
    66421 atcctttaac tgagcgaagt tctcgcaaaa aacaatatga tatagcccgg aacataatta
    66481 ttaatgaacg agtggaatcg ggaattttta tcttttatga agagaataaa tccttccgtt
    66541 taagctttat ttatcccatt tatcagggaa gacagagaaa gtatagcaat tataaaagaa
    66601 attcattctt tgtgagcaag gatgaaccta ataagaccta tattctgcaa atggaaaact
    66661 atgatatgca ttctattaat gaaattaagg atatgttttc catagaaaag gtctccaacc
    66721 tattttataa tgaattccaa aaagaatttg aaagactgaa aaatgaagtc gtccaaccta
    66781 agtttgaaga agttatagat gaaaatcttc gcaatgactt tgctcttctt tttgtcctgc
    66841 gcattatttt cctcggattc gtgcagaaaa aaggatggct gggaaacgat gctaaattta
    66901 tcaggaatta tatagattgc tatgaccctt gcctacatac caatggtatt tatcagaatt
    66961 tattagaacc tctatttttt caagctttaa actctcctca ttctaaaaag aataaattca
    67021 ccttccccaa tattcctgaa ccttttaata atgctttagt taatgctcct tttcttaatg
    67081 gtggcttatt ccataaaatt acagattttg acaccatagc attatatatt acaaatgaag
    67141 gtattcgtaa ctttgtagat ttccttttca gttataactt cactctggaa gaaaataccc
    67201 tctatgacga agaactggaa ctaaatccag agtttttagg tattatcttt gaaaaactca
    67261 tcaataaaca gtatggcgca atttatactc cccaattgga agtagattta atgtgtcgtc
    67321 tttctctggt taagtttctt cagcaaaatt gctcctccga tatatcctat gagaatcttt
    67381 ataaactgtt cttcagagag tttggtggcg aggaagaaca aactcttggt gtcttcactt
    67441 atgagcaagg cagagaaatc ctgcataaac ttgaaagtat tactgtttgc gacccagccg
    67501 tgggctctgg agcttttgcc gtaggtatgc taaatgtcct gtatgacacc gaagaagaga
    67561 tatataaaaa catcttaaat gaagatatca ataccacccc ttttgaacgc aaaaaacgca
    67621 tcattttcca atctctttat ggagttgaag tacaaaaatg ggctgtatgg attacccaat
    67681 tgcgtctctg gatttctctc tttctggaag cagaagatga actcaaaatt agtgatgaac
    67741 ccttattacc ctcttttgat tttaaaatta gacagggaga ttctctcatt caaatcttag
    67801 gggattccct tttccctgta tccggggaag gtatcatagg tcctgatatt aagaaaaata
    67861 ttgataaaat ccagaaactt aagactgaac attattataa taaatccgct cttaccctta
    67921 atgagataga aagattacaa agaaaattat acattaatat gctggaaaag aaaaaaaata
    67981 ccctcgtaaa aaaactttat agattacaga tagataccac tcagattcaa gctgatttct
    68041 ttagcgatga agaagattct gttttagaac tggctaaatt aaactttgaa aaaagtatta
    68101 aagaacttga acttgagatt aaaaaacttg atttctccat agataagatt aaaaataatg
    68161 acatcccttt catctggcgt attgatttcc ccgaaatctt tttatctaaa gaaggttttg
    68221 atattgtgat tggaaatcca ccttatgtgg ctcaagaaga aataaccgac ccccaacaaa
    68281 ttatagataa cagaacatat aaagctttat tgaaaaagat ggcagctcag gatttcccgg
    68341 atagaatttc tgaaaactct atcagtggta aaagtgattt atacactttc ttctatatcc
    68401 gtggcttaag acttttaaat aaaaaaggca tattaacttt tatctgttca aattcttgga
    68461 tggatgtgga atttggtgct tggttgcaaa aattcctact ggaaaattgt caggtttatc
    68521 ttattattga taatctcaat aaaagagtat tccgggaatc tgctattaat actattattt
    68581 ccgtgataag tgctccacaa ccaagagtga aaaatgatga ccttatccga tttgtcgctt
    68641 ttaaaatccc ttatgaaagt tcaatttaca ccgataactt cctctctatt gaagatgcca
    68701 gaaaacgatt agatattgat gaccttagag ttaatgtgct aactcgtatg caattgatgg
    68761 aagaaggctg ggaaaatagt gaagaaaatc gttatcgcgg ctctaaatgg ggatccatat
    68821 atatcaaagc tcccgatatc tatttcacta ttatggaaaa agctaaaaat aaactggtta
    68881 aattgggtga tattgcagaa ctaaaaagag gttatactac tggggcaaat gaattttttt
    68941 attttgcgat aaatgatccc agaataaaac tcttaggaga agataatttt aaagatataa
    69001 tcagaagtcc acgagaattg aagggaattg atatagataa aaattggtta aattataaaa
    69061 ttttgtatat aaatcctagt atcgatattc tatcaaaccc ttatttattt caatatatta
    69121 aagaaggtga aaaaagaaag tatcatctaa gacctactat taaaaataga attccatggt
    69181 attctttacc acctcaagaa ctatcggata tagtacaggg acagattata aatgatagat
    69241 ttcttttttg cttaaatcaa aaatactatg cggattgtgt cctgaattta ttatatctca
    69301 atgaggaata tactaattta ctatatgaaa ctcttttatc tcttaatgca tcaataacta
    69361 tattaatgtc tgaattaaat ggtagaacag cattgggaga aggagcattg aaaacacaag
    69421 tttttgaatt aagaaacctt aaaattactg atccaaaatt aatagctaaa tttccagatt
    69481 ctcaaaataa aattaaaaat atgatgaaaa gagaaattta tagtgtttgc aatgaatttc
    69541 aattaaactc ttatcaggaa attaaagata aattaccaga tccattacca gatagaaaag
    69601 ctatagatga tatagttttt tcagctttag aattaacccc aaatgaaaga aaagctgttt
    69661 atatgagttt attagaatta actgcaaata gattaaatag agcaaatctt aatcaagaca
    69721 gagaataaga taataaataa ggagagattt tatggaaacc tatgagttac ttcaagagat
    69781 ggaaagactt tttttggttt ttattagagc gaggtcttta actacttatg tttcagaaga
    69841 tattattgat tatgtacaag gtaagaacaa cactcatatt atcttagatt acgatacact
    69901 ttataatgtg tatggatata caacctgttt atttattgag cctaaaaact tttctactcc
    69961 tgaaaagtat aatgaatata aaagtatggg ttcctggatt aatgcaagct tctttataca
    70021 tttgaatgta ttgattgaaa aatatattaa agaattacca atagagaata aaaaatcgtc
    70081 tgataaaatt acagataatg attattataa aatccttcgc cttctgcgaa ataaagttgc
    70141 tcataatcct tttaacaata ttaaaattac aacaaataat aaagataatt gccagtgttt
    70201 tgaacaatta attaatacct attttcaaca atgtataaag gaaaataatg aattagatat
    70261 atccattgat aaatttgtaa ttcctctatt ttggaaaatc tataaattaa taaaaactga
    70321 aattagttaa taacctatga gcctcttgta ggaattgatt aagtaagata ttttttctta
    70381 tagaaaatag ttgtgccatc ctggtagttt tagaatggca caacctttga ttgatt
//
LOCUS       DAORZF010000005        56455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_484_length_56455_cov_390.811968, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000005 DAORZF010000000
VERSION     DAORZF010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 56455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 56455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56455
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_484_length_56455_cov_390.811968"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3834)
                     /locus_tag="PK015_01460"
     CDS             complement(<1..>3834)
                     /locus_tag="PK015_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gingipain R"
                     /protein_id="HOL33174.1"
                     /translation="HSVFITCNTGTFNSGTSTTEAVVRYGTAASLGGAITSIGMATSS
                     THTPMNNCLTVGIFHGIYPLGMRNMSEAMLYAKLYLYAVYGASQSTQAYNFSSYCNLI
                     GDPTASVYVGIPSTFISNAPTSIPAGSSTLEITVLNDHDQIVQGASVVLSNENGQQAL
                     AYTNAQGKAYLEFASNLSGSLTLTINKDDFKPLVSTINIVSSGGIVFNGTELDDNMWG
                     NGNSLLDAGETVETYVSLKNTTNQVLTPAVIANCSDPYVTLILASRIQYNNINPQASG
                     VNIDPIVFSVASHCPDQHQFSLKLTVETTAGSWEVYVPFTVHNGNLNIASYTFIGATN
                     NYIHPGDNLPMTISLTNVGSLGLNGINARLRSRDIYFTITDSIGTYGSIPVASTVSNN
                     SDTFSVFARSTCIDGMVIPLELYLYNSAGYKETIYFTVTIGQTTIHDPLGQDAYGYFI
                     FDQGDTGYDQCPTYNWIGIAPAEGGSGTLLPLYDPGNTSDEGDQTSAVAITTVNLPFP
                     FKFYGIEYTQASISSNGFIAFGQTQDADWRNWRIPDAGGPSPMIAVFWDDLTLGTGSG
                     VYTYYNATLHYFVVEWYHMISGYNNSSLETFEAILYDPIYYPTYTLDGQIKLQYQQFN
                     NIDLGSGDTLPHGNYCTIGIEDHTETIGLEYTFNNTYPTAAAPLSHNSALFITTRPIL
                     PDYPYLVLEQIFVIDPNDNAHLEPGENADLSIRLGNRGLVNATGVSAVISSSDSYVTI
                     LNSSANYGTIPAQSSGYPQTNYSIFVSPNCPAHHQINFNIYITSDTGNWNYNFPLEVV
                     VPELGFNNMTINDSGGNSNGILDPGETVTVNIHIVNTGVIPAPPGTVTLTCNNAGITI
                     INGTDTFPVLEPAAFEPLSFTISAASSMSTGTLINLIFNAVAGTTTAQHSETVEVGAP
                     IVLTIGTGTGTQPYPLDRYYNYCAHEAIYLASEIGSACTIKNLAYYKASGADVSPIES
                     VTIYMKNTTASTLSSGTYSTSGYTQVYSGSFPNNSTSGWMEVTLSPMFVYDGTSNLSI
                     LIVKGHQQWISNYPMWTYTSTSTSRARQNRNDNEAPTNLSATNNLPNLRLKIFPQVES
                     LLPPQNFTAIPSHQSVYLSWSAPVSGNPTAYKIFRNSSSIANVTSLTYTDFSVTNGVS
                     YSYYIIAHYSNGESSPTSTITVIPNAYPPTNLTALAGNATVTLNWNPAEGRNSPRDNT
                     KERLISCYRVYRNNVPIQDVTETTYQDTGLVNGTTYSYYVTTVYINPAGESAPSNTVE
                     ATPQMVSIVVLGSGTS"
     assembly_gap    3835..3934
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3935..4617)
                     /locus_tag="PK015_01465"
     CDS             complement(<3935..4617)
                     /locus_tag="PK015_01465"
                     /inference="COORDINATES: protein motif:HMM:NF019737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family peptidase propeptide
                     domain-containing protein"
                     /protein_id="HOL33175.1"
                     /translation="MPKKTFKYLSLILILLAPLFLLAETSVAPFNIVNISDNSIQILF
                     ELPAWSMERSEINNLSRVKIDDIPYLFLEETETLPVFSTMIAIPYQGGVNLQINTTQQ
                     SIQNHIRLDFDSLLSSEKASGRYNEELYPANSVVISEPQIMRDFRIVTLNIYPFQYDQ
                     SKQTLSISESIDIQLNYDNQPSINEIEPPLNLSPAFEKIYPALILNYEQFITRDEITY
                     SRPRMLVIYG"
     gene            4814..7042
                     /locus_tag="PK015_01470"
     CDS             4814..7042
                     /locus_tag="PK015_01470"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOL33176.1"
                     /translation="MRQQLNLGIDGMHCAACSARVEKALSSLKGVISASVNLGLEEAS
                     LEYDDRYLKVEDIVKTIESLGYSVRESIILTEDEQIQKVQIARKNMIQMWIIAIIVMA
                     IMLIMMASHEMPSWHKAANWIIFSLTILGMCFPARKVYISAFKAISTGGTNMDVLIAI
                     GTIASLLVVPLSLIVNGIRADDFSGIAAMILAFHLTGRYLETKARGQASSAIRKLINL
                     GAKTALVLIDDEEKEIPVHKLKVGDIFLVKAGAKVPADGIIIEGSANLDESIATGESL
                     PVYRQAGDRVLGATLNLDGFIKVQAERVGKDSFLAQVVKLVEEAQNSKVPVQLLADKI
                     ISVFVPVVLTLSILTFFAWLLFPSFMSNISEAIRTIIPLSSANYGLAAALMAAIATLV
                     IACPCALGLATPTALLVGSGIGAERGILIRNGEALQRMKDVKVIVFDKTGTITYGNLK
                     LLKIVSPKLSEQEILQIAFSLEQGSEHPLAKAIITEAQVRDLQTFPIEEFTNYSGKGI
                     SAKINSQSYYLGNLALLKEKGIKIDEFNLKDTQYPYATSVFLTDEKQVLGILYLADTI
                     RPEAFEVVKTLREMGIESILLSGDNQETTAAIAAECGISKFIADALPGDKAKIIKELQ
                     EEYGALAMVGDGINDAPALKQADIGIALGQGADIAIEAADIVIVPNKLELIPRAIKLS
                     LATFQKIRQNLFWAFFYNVIAIPLAIFGALHPIIAEIAMAISSVTVVSNANLLRRELR
                     HL"
     gene            complement(7058..8311)
                     /locus_tag="PK015_01475"
     CDS             complement(7058..8311)
                     /locus_tag="PK015_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HOL33177.1"
                     /translation="MNSQLKGKRLLGINGVGRIGKLLLWNQIHLRHFDGIVINFGREV
                     GKSLDDLIQVLEMDSTYGSIHKFLFGVVGRKAEIKVIDPEKPVLEIEGMPITILRKAR
                     DPKDINWLEEGVRIVVDCTGNFLDPNITPEKGVPCLRGHLAAGALKVICSAPFKSKAA
                     VMEDNPDTITMIYGINHLNYDPQKHHIISAASCTTTGLAHMIKPLLEHKETANIMTAS
                     MSTIHAATNSQSILDSVPKAGASDLRKTRSVFNNIILSTTGVSKALELVMPEIKNIGF
                     MADSVRIPTNTVSLINLNVTFHAELNELGQPNITRKLINDIYKQAAEGPQNGLLMYTE
                     RQNVSADMIGNMAAVVIEANETHTRTSFIAVPPKTLKELGAPEDKTVYMPVTHAKIFG
                     WYDNELGSYTNCLSRLINYIDETTP"
     gene            complement(8349..12416)
                     /locus_tag="PK015_01480"
     CDS             complement(8349..12416)
                     /locus_tag="PK015_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOL33178.1"
                     /translation="MDKIQSQALAENLAQTQVKEIVLSPESQWLLKQSTNYFGIQQRT
                     KQFLEEFHHPYANWDEVLFLLRQSVIGDLWFYLQLPERDKALSIILRIFEEIFQKAEK
                     KETKQKVITEFLSFVSALASQEQVPQIILEASGEILEKWYRDYPDLFIPLTGQCYSTL
                     KLLSTRISHPERLLVLLKSIYQSSLELWKSSSSIEEWYDKYADLITLSRSYLSRTIGT
                     RFYNQQLNQLGKIKSIEELNEIPSFTEIAVRHRDLLAQFPSIQEKIHYIFYLLSLPPM
                     SGLIDHLLWDFNRILGQLRETLSAKEINALLDSVFEFLAEYKDVHTSIVLDCVLTIGK
                     AVLDPEEPELGKRLIRKIIDLGFIPPGKIKINRDWQIIANKNHIKNIRVWLQLISIDP
                     AFCKDLLAALTISIIRQGIFVSDTDLFQKDVSAFLNSNIKPIYVQAKHLLRLLPVFYN
                     EIGAEGEIRDLSTEIDELCYRKDLLMHFVRKQVHTESNNTQIQLLQEILNYWVDLDPN
                     HLKGLAPEDIEEYISHPDELNQKQHQAVNKFLSDHNLDAQNLLNQSWQSIASLFKSIK
                     NEDIPLKKLELLCRIYYLLLDKYQLDPYDITKFLSRYNWFDQKEQLRLKQSLARNDYD
                     SSIHQMLNYIGRLNKIVLDPKPTQPWENIYYKRHIAAGIPSMYGQYREPKLEAMGIIF
                     RLENLVERLIEKDIKQLNLEYLTAKTLKRIIHILELYELGLIYEGYSVSAFSNALEML
                     KSSQRVVRLSLDQYLDLFKQIKDSITDLINEHFYRFYEQELSHLPAKGIPGKYKKQTI
                     QQMFAEEFFRKLLGSSFLVQSMDNFISQIITSLNSMKTIFAPEDLIKVMSYDPDLLFV
                     PLHTNNRRLDNQVLLGAKAYFLKKMIQYEFPIPAGIVITTELFRARNIINAHPEIEKE
                     LDEKIRFNVNRIENYTGLEYGNPEAPLLLSVRSGAPMSLPGAMSTFLNIGMNDEITLK
                     LSTMPNFGWTAWDCYRRLLQSWGMAYGIDRDEFDAVMISYKRRYKVQLKTQFTPEQMR
                     DMCTSYKKVLARHKVSFEQDVFLQLKQAINHVLDSWNTDRARLYRQKLQIADDWGTAV
                     LIQKMILGNISLDSGTGVAFTYADWSKEPGITLNGDFTLCSQGEDVVAGLVYTLPISE
                     QQRKLSKSPVQVSLEKDFPDIYARLLSLARQLVEERGYPHQEIEFTFEGPRKEQLYIL
                     QTRNQLITKVKQYTVFSVPKRELKLLGNGIGVGKGVLNGIIVISRQDIDELKDMNLPL
                     ILVKPDTVPEDMELLFDCQGLLTSRGGVTSHAAVTAVRLGIIGVVNCRQLKVIEEDNI
                     CRIGDYILRPGDKIAIDASSGSIYLGHYPVETIPG"
     gene            complement(12538..15294)
                     /gene="polA"
                     /locus_tag="PK015_01485"
     CDS             complement(12538..15294)
                     /gene="polA"
                     /locus_tag="PK015_01485"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOL33179.1"
                     /translation="MRDKLYLIDGTALLYRSYYAFVNNPLINSKGENTSAIYGVVNSF
                     LHLLDSKKAENLLISFDRKAPTFRHELSEAYKANRPPMPDALLAQVEPVQEFFRIIGL
                     KEISIDGYEADDVLATLAERFKTDYDIVFVTIDKDYAQLVDDKAVIYDPMKNITLDKE
                     AIVKKYGILPEQFVDFLALAGDPSDNIPGVRGIGEVTAVKLLKQYSNLDEIYAHLDEI
                     PEKLRQKLIEGKDNAYLSRQLAMIIRNVPIEIPRIEALKFNPESLHKAKDFLTRYEIN
                     TLKRLIDNRYGQKIAISIPSEEPDKLVQSDIFESKYTVPSKMGNKKFQAILATAENYS
                     KLLDDLKKVNLVSLDTETTSEDAMQADLVGISLCFKSDEAWYIPIGHKEYNNFPKEQT
                     LRELNEALQGKLILGHNIKYDMIVLHQEGWNWDNKIGDTMIAAYLLDPGTNQYSLETC
                     TATELQYEMITFSSLINKKACPSFDLLEVEKACDYSAEDAWAVYMLYPIYLKRLENTG
                     LKELYENIEIPLIPVLQQMEENGVSIDTEVLKEISKNINQELEKLTEEIYAYAGYEFN
                     INSTQQLAKVLFEEKKLPAKKKTKSGYSTDSAVLEELAEKYEIAEKLLQYRMLSKLES
                     TYVSALPKLINPRTGKIHSSFNQTVTSTGRLSSSNPNLQNIPVRTDLGKTVRKAFIAT
                     DPDWELMDADYSQIELRLLALMSGDQVLIDSFKQDLDIHRQTAALITGKPLNDVTDEE
                     RRRAKVINFGLLYGMGQKKLSRELGISQEEAKKLIDEYFHRFPTIQAFINNCKAEARL
                     HGFCQTLFGRKLYLKNINSSNQGLRAEAERVAVNMPIQGTAADLIKIAMIDIHKQIKD
                     NPRIKMILQVHDELVFEVHKEFLLEAEKLVKKAMEEALPEKYSRIVTLKVDIGHGHSW
                     FNAH"
     gene            15484..16128
                     /locus_tag="PK015_01490"
     CDS             15484..16128
                     /locus_tag="PK015_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HOL33180.1"
                     /translation="MALVLKTTKFVEAQIDTFLDIVSDSALIFRFAVDDYLQERTSQF
                     EERLNLIRENEKKVDDLRIAVERYLYERTLIPENRGDVLAILENTDEVMDHMKDTLMQ
                     FSIENPEIPSVLDDLWLQTTTASTAAVEQLVFAVRSFFRDLSQVNNFIHKVYFFEREA
                     DQLGEKLRRQIFSLDIDLALKSQLRWFAIHIEQISDYAQAVCDRLAIYTIKRQL"
     gene            16142..17638
                     /locus_tag="PK015_01495"
     CDS             16142..17638
                     /locus_tag="PK015_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOL33181.1"
                     /translation="MVYFYLLSGLFIGWSMGANDTGNIFGAAVSTRMIKFRQAALIAA
                     IFYTLGAFLEGSGPSGTLGRLGSIDAIGGAFTVALAAALAIVAIVRLGIPVAVSQTIV
                     GAIIGWNYFSGRITDFNSLITIASSWIIAFIVSAAVAALFFYLMRRWINRSRLHLLEQ
                     DMIIRYALVVFGAIAAYFLGANTIANAIGVFVPVTPFKDLTIGSMFSLTGVQQLYLVG
                     ALSVVLGIYTYSHKVMGTVGHEIYKLSPAMALVALLAETFVLFLFSSRGLHNLLLSIG
                     LPAVPLVPISSTQAMVGAVLGIGLAKGGKNIKYNVLGKVSLAWIVAPLMAFLLSFVAL
                     YISQNVFELQVRLPITYTVDKAAVKEIEQRGIDTKHLSFVNLRTFKTERELYKELMEE
                     DFYTPEEVKIIISICENYPLLVNVELLNKGGLNRRFTEEQLKALSELDGKKFKRKWNL
                     AQALSEYPAWKFIEPPQTRADEEHNEDLKSDLEIIYKVFYFPSDNNNR"
     gene            17654..18553
                     /locus_tag="PK015_01500"
     CDS             17654..18553
                     /locus_tag="PK015_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOL33182.1"
                     /translation="MNFFQGQNLTFILCFFIGFILKKLKLLQKDDAHILLRFVLNVCI
                     PPLMILAIYRATLALDLLLVALCAILVMVIIYFISSAIGKKLKMEPQTFGSFLVATMI
                     MNTAFALPYFKIIGSEELARASLFDIGNTIMIFTFAYYQAIKYGTSATKEKIQLKKFL
                     TLPPLYAILFAVLIKMSNLQLSNTVISFLELLGNPTGILMMVALGLACEPKLKHLGKA
                     LIAVVLRMGVGLITGYLLTLIFRLHGTAQIVVIICSSLPAGFNTLILADRENMDRDFA
                     ATVVSISTFIALFTIPLLINFFY"
     gene            18882..19319
                     /locus_tag="PK015_01505"
     CDS             18882..19319
                     /locus_tag="PK015_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOL33183.1"
                     /translation="MKLVPSRRTSEINPMSTMLNMFDEFFNRAFEEDFEEENFRAMPM
                     DIIERDKEYEILANLPGFKKDNVKITVHDNQLLIEANVEETKEEKKGTIYRCERYSGN
                     YRRNLLLPDNTDVSKISAKMEDGVLHLIIPKKEPTPQKEIVIQ"
     gene            19480..19863
                     /gene="gcvH"
                     /locus_tag="PK015_01510"
     CDS             19480..19863
                     /gene="gcvH"
                     /locus_tag="PK015_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOL33184.1"
                     /translation="MLVPDDLYYTETHEWVRLDDGIATVGITDFAQKELGDIVYLELP
                     EIGNKVSAGEPCGTIEAVKSAEDLISPVSGVIEEKNMEAEESPEIINKSPYEEGWLFR
                     VHLSNLEELEDLLSPRDYTKLIEAL"
     gene            19867..20484
                     /gene="gmk"
                     /locus_tag="PK015_01515"
     CDS             19867..20484
                     /gene="gmk"
                     /locus_tag="PK015_01515"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOL33185.1"
                     /translation="MIKKNKNFLIILSSPSGGGKSTILNEVRKIRPDIEYSVSYTTRP
                     PRGDEKNGVHYHFIDEEEFLRRREEGDFLETAQVFGHWYGTSISFIKDRLAAQHHIIL
                     DIDVQGAAQISSTSIPYVKIFIMPPSMKILRERLIKRNTDSLREIEKRLLVAKQEIKF
                     IPQYDYLIVNDELSVAVEDVLAVIRGEENRTNRYPEPIKDFLEEE"
     gene            20490..20921
                     /locus_tag="PK015_01520"
     CDS             20490..20921
                     /locus_tag="PK015_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33186.1"
                     /translation="MYNPKIDLFLEKTGIDYLFCLLANLEAQRLSQLPAYVRQHFPDK
                     IPVMAMNHVADNEVPDYFKEIEEATLKMAKEHLPYPEEEVELEEETYDDSGEHIEELA
                     DSTHRFYSEEEEEEEIEETEEEEEESYEFEDDLDNFEDEEE"
     gene            20931..21572
                     /locus_tag="PK015_01525"
     CDS             20931..21572
                     /locus_tag="PK015_01525"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOL33187.1"
                     /translation="MIPRELIQHLELLKNLGVKEIFLPTSRKAEILKELSVKYSTCTK
                     CKLHTGRIKFVYGEGNPDALAMLIGEGPGEQENLTGRPFVGPAGNLLTKMLKAINLSR
                     EDVYICNIVKCRPPNNRNPEPEEREACLPYLLEQIEIIQPKLILILGLVAAQTLLNTK
                     LSMEKLRLTTHSFKDIKTFVTYHPSALLRNPNWKKPAWQDLQRFQAEYIKLLQ"
     gene            21569..22372
                     /locus_tag="PK015_01530"
     CDS             21569..22372
                     /locus_tag="PK015_01530"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOL33188.1"
                     /translation="MNAGSLYKKLLLSFFLVLFLSSIYAVIAIYGDSQGNNDIHKQIV
                     EAIIKYQPDITFHLGDLTAQGKKQEEYDEFFSCCKPLTDLCPLYPVKGNHDASSELFL
                     KNFPFLSQTYYTVEYDSLLFIILDSTLELSPHSEQFNWLIETFNSNPLKPKIILMHHP
                     IFSSGYHSGNEDWALYLPALFASNKVIAVFSGHDHNYEHLQWKNLNFFISGGAGGSLR
                     PSLSQHPYSKIFCSSYNYLILNRQKNYLICTAYSLDGDIIDKVVIFLDF"
     gene            22955..23830
                     /gene="dapA"
                     /locus_tag="PK015_01535"
     CDS             22955..23830
                     /gene="dapA"
                     /locus_tag="PK015_01535"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOL33189.1"
                     /translation="MLQGSFVALVTPFKHGEVDYVALERLIDFHLEKGTDGIVLLGTT
                     AEASSLASDEREALLLFAMQRINHRVPVIIGTGSNNFNHTLADTKQAQELGADYALII
                     TPYYVKPTQKGMEEYFLAIAEKTNIPIVIYNVPGRTGVNISSTTVVKLARQCSQIAGI
                     KEASGNLIQATEIVRDAPEGFALLSGEDAMNFPLMAIGGKGCISVTANIIPQELSEHI
                     HTCLNGDFITAAKQHQHLLKLHQTMFIETNPIPVKEALHMMGMLELEFRLPMCPLMDN
                     NREILHNVLSEYKLI"
     gene            23840..26761
                     /locus_tag="PK015_01540"
     CDS             23840..26761
                     /locus_tag="PK015_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOL33190.1"
                     /translation="MKKQVEKYGFKLLETREIKEISATAYYYEHKKSGAHFLNVKCED
                     NNKVFCVTFKTVPQDNTGCPHIMEHSVLNGSKNFPAKSTFMELIKGSLHTFINAMTDS
                     DMTLYPVASTNEKDFYNLSRVYMDAVFFPKIYEQPHILRQEGWHYDIKDPEDELKIIG
                     VVYNEMKGAYSSPDNLIELNNQIAQFPDSPYKYDSGGNPKEIPQLTYEAFINFHKKHY
                     HPSNSYIFLYGDADENEIMEMMDKEYLSQFNKDVEPVEVPFQKPFTRTKKLEVEYPVE
                     EGKDISEQYHLSLNFTYGKNPDLITVSAINALMDILMSTPASPLKRAILESGLAHDVR
                     VSLNIGILQPTFSLICKEVKKENIPLLVQLITDELNRLVKEGIDKKLIEAVLNRREFF
                     LREGPSGNFPKGLYYAFNIYHHWIHGGNPIDALAFEDILKELRRGLTEPYYEELIKKS
                     MLKNHHSSQITYVPVPGLANKWEQEIREKLAEEKNKLSEAVLNKLIEETREFQQWQLQ
                     QPTPEELARIPFLTLEDINPEAEAYPLEIEKLGTKTILTHPLKTNGIVYLKAYFDLDH
                     SSEEDLPWLSLYASLMGMVSSKNYDYIDLSNEIYSHTGDLNLKLNIRPSYQDPDVILL
                     KFLLSGKALYNKTEKLIELAKEFAFYPLFDDYDRLKLLIREAKAKLQTRLFYFAESIA
                     TNRLFAPSSQLHHFLDLTSGLDYYRFLSDLDKKLEQDIEIIAHKLEQIRDTYFLQNNL
                     LISITAEEEAIKDTINGLNPFLDAISDKVLPPVERYFHPKELNEGIIAPIQVQYCAKG
                     GNFFRKGYSYSGKLRVLTNILNSDYLYRELREKGGAYGAWSGFTLNGYMYFVSYRDPN
                     LQRTLEIYDQTSEFLRNFECSKRDMERYIIGDISNLDYPKTPEGKGAQSDDDYITGFT
                     QEDRQQIRDEVLSTRLEDIRQYADMIEEVMSMNHYCVVGNEAKLKAASELFDRLTPLL
                     S"
     gene            26766..27830
                     /gene="rsgA"
                     /locus_tag="PK015_01545"
     CDS             26766..27830
                     /gene="rsgA"
                     /locus_tag="PK015_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOL33191.1"
                     /translation="MKPKDKKILKPHRPARLKNTRLLDLEVLDELPDNIKFKAKRTPE
                     KVAETYKKDMQLISGRILEIKSNYQYTIEADNKTFTATLSGRLKQFAFHSKSIVAVGD
                     YVELDIVAEPPRIENILPRKNTLTRYLGSNFQRPTILAANIDQVIVTSSWRKPMIKPG
                     LIDRYLCLAALDNIDAIIIINKVDLCEDREKLEETVSYYKNVGYTLLYTSALTGEGID
                     ELYELLKNRDSVFSGQSGTGKSSIINRLEPSLNLPTAEVSDYNEKGKHTTTSTILIPW
                     SFGGHLLDTPGIKTVNLAKDAKSQIPRIFPGFATLAPLCYFNDCTHTHEEHCAVLSAL
                     EEGKIPTNRYLSYVSLLNSL"
     gene            27830..28681
                     /locus_tag="PK015_01550"
     CDS             27830..28681
                     /locus_tag="PK015_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOL33192.1"
                     /translation="MKNFVIYINQAYKDKTAIYDLLEELKSEDHLNFYGIPSQQEILP
                     PDIHILESDKTPKIDCVLVFGGDGTILRAKNIALRTKAPILGINIGYLGFLSETTLGE
                     LKSSLRDLLNKKYRLQERMLIQCQLRRNGAIIYKDLALNDAVACKAEMPKLIGVRIFN
                     NRRFVFDTRCDGVIVSTPTGSTAYSLAAGGPILSPEMKAMVLSPLNPHLLTVRPMVFP
                     STDHILLRIHNLEAPATLQIDGVNCSPIEENDEVAITAAKQTVQFVKLSNRTFYQILR
                     RKMHLGK"
     gene            28682..30376
                     /gene="recN"
                     /locus_tag="PK015_01555"
     CDS             28682..30376
                     /gene="recN"
                     /locus_tag="PK015_01555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOL33193.1"
                     /translation="MLTELYIQNYLFVPQERIEFGPGMTVLTGETGAGKSILVGAIAL
                     IFGDNSSALEPYDETKPIYLEATFDISHNSEVQSFLQQQGQEIDSELTLAREIKPAGK
                     STYFLNGRKVSASLLRELKPLMIDFHHQRDQQRLLSNAYQLQLLDLYAQNSSLKKEYA
                     QIYHSLKSALKELEKLKEEEEKNRQLKELYQYQFSELEKANLKMGEDEELQKEFELLS
                     NAQDLIETASSAYFTLYESEDSIHSRLAQVINSLSSYSSIYPRIAPILENLKDCLEQI
                     SASASDLNDISQSINLNPQRLQEIQLRLDEINSLLYKHRAKNIAELIELFQQREGQIN
                     AMEDLSATIKALENSIQQDYENLKELGEKLTKSRQKAADSLAKELQENIRLLSIPDAL
                     FKINIDKKTIENMLLEQYLSVCSDEGEDNCQFLFSANRGTDLKPLSAVASGGELSRIL
                     LAIKKVLSERMEEKLIILDEIDAGIGGKTADYVAQFIYQLAQHHRIICITHLAQIAAI
                     ANEQLALQKVPAKHKNVIRIISLEPQQRKQELARMLAGDISEISLKHAEELMTKYK"
     gene            30384..31307
                     /locus_tag="PK015_01560"
     CDS             30384..31307
                     /locus_tag="PK015_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33194.1"
                     /translation="MAKISSTARKRIKGIAIIIIFLSLGWAIFSAMEQKDSGPNLNEI
                     FKKSNKTFEETHAIKKAKIRISSPNIANALQQMDAIVYSYAVDTPLLKSRESSYGEFI
                     FKVDSYKMPELTQKLASLGNIEQQIEVADTSLVNKSLATEEEILKSKRKELADLEAAG
                     NIYGSLADYKNRLITEIRVQEDRVKALRQSDITLLYVQLIPSVVSNQFSWAKTFALNF
                     VKALVILVVGVVVLYYMLRLIAYILSLFGVKGFSASTLGSSYRYGDYYNRYYSRYGYG
                     RHRRKVKRIYKDGRTNDQSENKEETKDEPKT"
     gene            31321..31758
                     /locus_tag="PK015_01565"
     CDS             31321..31758
                     /locus_tag="PK015_01565"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOL33195.1"
                     /translation="MQPWQIWIILGIIFTIIEIFDPAFFFIALGIAALITGLLSLFYI
                     VGSNVLLQIIIFSALSFICFLLTRKIGKKILQFPTAETNIYALKGKTGFVTKAIPQDG
                     KGYVKVGGEEWSAISEDGSPIEENTKVIVLGVEGNKLIVKKAQ"
     gene            complement(32404..34236)
                     /locus_tag="PK015_01570"
     CDS             complement(32404..34236)
                     /locus_tag="PK015_01570"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOL33196.1"
                     /translation="MDSLVKKTYLLPTVRVIADKPEDAIGALHIIDYIEQKGIAALNL
                     YEGLQDIAGVTNTTGTKDESNLRIRGFRSNEVKVLVDGRPLNSGYFGNIDLHQFSPSD
                     IKEIHIIKGPSAAIYGTNTMGGVVNIITSDPGKDSWLKLNLLAKRNNANRFEISSDHK
                     LKKFGYRVSLAREHNEGIVLPKDFTPTPFENGGVRNHSLKTQYDFQTRLDYEFNPFRQ
                     VGASAGFTYVPEKLIPSSIYTLDYRQYTDWMHYWTTLEYVDILNEFLKLSSHLYYDGG
                     QDIYREYSDASHQYLILDSHMRYYTLGFNPRMEWVPDAWNTFNFGFRLESMHSTRKDN
                     GNYLQWTPHWLNIYNAFCQWKYQLNNKVNFLAGLGASSHHCDVKSSLSFFPEPSAALN
                     FKWNELSKSTISIGNNISFPTMRQLFSAENGNPNLKPQNAIKCEISHQQKIKAGNIIF
                     SNQLSLFYNNTRNLIDRQGDTYQNIYKVHSAGTEYSLMFKPFAWWESEVAYSYIWWEG
                     TENYELTETPHHQANFTQNLSLPWKLKLRYTCSYNDNRFSQDKLNNYHILSSYWLHNI
                     GLSYKFKKFSLAVGLENIFDTYYETEYGYPGPGLNFFTRLNTEF"
     gene            complement(34320..35132)
                     /locus_tag="PK015_01575"
     CDS             complement(34320..35132)
                     /locus_tag="PK015_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33197.1"
                     /translation="MKKLIYLLVLLLLLAFTACSEKDNPSEVKILGYKLDQFINPDTV
                     RIHIDPQAEADLEYRSLFAYEIVSSTDGFSPRQSAYAGYDLSWETFSQGYYVPNDDHR
                     TWFPGMGLPSAFKVRNAGLFRLYRKVDVDAGNRGSKLIELRGLSTYTVDNWSGTAEDA
                     IKLSDLLQGIAAYDSVAFVAVDGYSKNYQPELINDGYYLLNSEVTTFPNFNSTLPGNM
                     KKFKKLAKINVYGATSAQNFDFALAPQEKADLTFTIPSDLSGFESTEMVTEK"
     regulatory      complement(35163..35281)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            35607..36707
                     /locus_tag="PK015_01580"
     CDS             35607..36707
                     /locus_tag="PK015_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOL33198.1"
                     /translation="MLAVKNLSRSFGDIKAVQNISFTADKGEIVGFLGPNGAGKTTTL
                     RMMVGYLQPDSGTIELDGENVFDNPLAASARIGYLPELNPLYNEMTVEEFLRFIAALR
                     KMKEPYLSERLEFVRENCGLDEVWKQKIGTLSKGYRQRTGLAQAIIHNPDLLILDEPT
                     SGLDPNQILEIRDLIRELGENKTVILSSHIMQEVQALCSRVIIIHKGQIIVDDSIENL
                     SSYISETTQIVLELEAENPDFQPWLEQYPQVQLTDIEPKDDHLALRFTIPIEMDLRKE
                     LSAFVSEQGWYLISMYRETQSLEEIFHLLTSEEPFYTEEEEKEEEEEVIEGEEISSEE
                     VNESEAEPEEETISLKETEEKAQEESSTKEQK"
     gene            36704..37426
                     /locus_tag="PK015_01585"
     CDS             36704..37426
                     /locus_tag="PK015_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOL33199.1"
                     /translation="MNPALTIANKEYSLANRSLSTYIIYGVYLLACGIWFALNALKIG
                     AADMRGIFGVMHTLFLFFIPALTMGSIAKERSTGTLELISTLPIKLGHIVWGKFLGVL
                     FQLVTVLLFTLVFVLLISLFGIGVDYGAIFTGYLGLLFAGAAFIAIGMFASSLTDNQV
                     LAFIIAFAISGALFIIGRIGNLIPLKLFATIEYFGFDYHLESFLKGVIDSRDLIWFAA
                     VTIIFILLAQFRLQAQNLSQER"
     gene            37428..39005
                     /locus_tag="PK015_01590"
     CDS             37428..39005
                     /locus_tag="PK015_01590"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HOL33200.1"
                     /translation="MNTRNQIITSYIIKILIVVLVVIISSFLYFRIDLSKSKSYTLGK
                     ATKETLHSLKDKVIVKVFASQDLPQELSTINRSLKDMLEEFARNSHNKFSFEYVRASN
                     NKELIEQAREYDIPAYSFTAYEDEKLVTKQVVFGLYLESAGKSSSMYLRPGMEQMMEY
                     QLLKQINKLHPDNLPELTVFMDSLSMVHQYTNYSDALATFFLELMDNYRITYTDLNTR
                     PKYTPVMLCLGTIDTLSTKQLYYLDQYLMQNGNIVMTQDRALMFLSERGSALALLDGP
                     IFRLLEHYGIRIEPNIVLDMECEYGRGSGLGTIVPYPFIPRLRPNPKFPYTAGFPVIT
                     MNIASEINPVPNAKIKFEKVLQTSNHSNVLIGPDFRVDIAVQKGLDPGYLSQPPKTVA
                     AEVSGTFHSFFHAALDDSTFYNSTDKSKIILFADSEFPLEFSAGAFIVLNAVDYLLGR
                     NDMIKIRSPRDVYNELSAEVYMQKHQMQPAEQEKTISILNRYFRLTAIFLPVLLLALF
                     GIIQASRHAAKIKYEEK"
     gene            38992..39930
                     /locus_tag="PK015_01595"
     CDS             38992..39930
                     /locus_tag="PK015_01595"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOL33201.1"
                     /translation="MKKNKIILLIVFLALLAVAIYLLSSRQKKETLKPIFPADSTSIA
                     KIEIANPDTSITFSKINNHWTIIYPIKWDVDEDRFKMFWNEVLKEKYSTEPVASGKAA
                     LDQYELKDSTALKIKVYDSKNKLLKEVWFSDLGNPADYFCFKGDNNVYQIKRKVCLNY
                     QPNLDSWRSPYALRIFPEQLYAIEVKHTKNSYQLTRNGNIWHYKDSKEDFDIPPGNVT
                     MGKILNALSRLGSGTIMSLDKAPELGSSACSVKLLLTDNSTIQIEFYPGEKNYFMTID
                     LYPDLVFEVPFDTYFRFTRFAATFRAQEGDPEELLR"
     gene            39951..40343
                     /locus_tag="PK015_01600"
     CDS             39951..40343
                     /locus_tag="PK015_01600"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOL33202.1"
                     /translation="MNENLYAEIISIWDKTGISNPERADSFDSIQRTLEHGGILLLAY
                     MDAILVGTIWLTNDYRRLYLHHMAVLPEFQKQGIGRSLLQEAIQIADKQGLQAKLEVH
                     SDNAAALNLYRSMGFKVLDGYLVMIRRN"
     gene            complement(40363..42270)
                     /locus_tag="PK015_01605"
     CDS             complement(40363..42270)
                     /locus_tag="PK015_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33203.1"
                     /translation="MLFFLRSISLTIILLLLISPIIHYVQQVNEKQQIILLSDISASM
                     DLPGGNGKTKKNWIQSYSSTIKDKFSSAGYEIISYDFASGLTNNRNNTLLVPALEELN
                     KKHDFSRIKGIVLLSDGWLHDAFPIPVKQLGCPFYALGDTATSLQADIKVVKVIYNPQ
                     AYRNEPTIIRAEIHSENYSGSAKISLFLGNTEVRNQTVSLKAGETISLDFDYRFSQTG
                     FYPFRVEVSVPGIKERSLNNNSYPGAIEVLTNKQRVVILSESPSWDNKFIVDAVTENP
                     RWEVKYYRVQGDKVFAGEKLVDTIPSDHLSAIIILNNGALQLSGYPLNYVLSSYQKGI
                     GILFQGLPLPELASILPLQKSNISSVYQGFLELTPQAARYPMLEFVSSELQNIPPLDY
                     YYVTSTKGAEVLATIDNPQNSPAIAVNTQGRAKVIAMAFLNLWKWQMQSESGGYKKLL
                     SNTLTWLANTSSTGYYAIYNNSYFLGEEINIRLRAEDNIRGLLLDLNPELKILDASGK
                     EVYRDFMIQAEGEYTTHFSLDKPGIYSFTISDQMSKQSVSGKFNVAESSLESMDFDLN
                     IPLLSWITSETKGKLLYPATLQSFQPLPAQNRVVNKSKDIPLYRKWYVLTLFIVAFCL
                     ELFFRRRWGLL"
     gene            complement(42366..43616)
                     /locus_tag="PK015_01610"
     CDS             complement(42366..43616)
                     /locus_tag="PK015_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33204.1"
                     /translation="MKKIIIGLIIFLTLSILSATTVTPKSMFFADSYMLRARGCDANY
                     WNPALLQRGYSEIMIPFFNNSIYLGNNSFNIDLYNYIMEQDYLDDEAKQKILNAIDGN
                     MSFSIGGNMGIIGFTTDNMSFTGSVHLAAKGALDEQFLEFVLYGNGDGSEVYHFTKNN
                     NYAEGLGYADLTYGIGDISLPLPETIPDIKFGFSLSALGGLGNVQTRKLDGYLSSNLD
                     GLNVQQDVIIRTGIGGYGLKAMLGLYSEPIPRLEVGATLDNLFGFIRWETQTQDEIYH
                     FEINNLYASNIDEDFYIEDSSTVDIKPYTTKLPPEMRLSALYTFKPVSVSADYVVGFG
                     NSPEVSKQGRISLGAQICPLPQIPISLGYCSGNEIYPWRMSYGIGLDLKSVGFGIAIQ
                     SIESAFFNSSTKGLALATYFNVRL"
     gene            complement(43616..45148)
                     /locus_tag="PK015_01615"
     CDS             complement(43616..45148)
                     /locus_tag="PK015_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33205.1"
                     /translation="MKKIALMLIAALLLLSACEISKPHLPTWDVELTVPLINERYFVS
                     DLADSVNLFVGENNVLTLIGTGETESQEFGNVSFNPEAILDPIPLLSGIVGNVTIPFI
                     DPTNTVELIYGLVDNGTLSYYYDLDDPANTQVSLSFPDIRTSSGNPLTISGSSYPYWQ
                     DVDLAGCTIGLEHSGQVLNALSVVITIQSNLPNGTPVGTAGLKIDQPLSFVAFEGYLS
                     DYQLDMEPGSVTINIDYPFGIDEAIQLQEASLCLLMNNEFGFGVKFYGELYGINHRTG
                     IEKTIPILDENGLPFYIAPANQSGSVITELQFSNRINEVLQIMPDEVNLINAYLLIQG
                     GNNGTAGFVNSTNRLRCTYQIDAPFTFILTDNPFTMEEPSKVEMPKDVRDQIGDRVTY
                     AAMVMKVKNQLPIGATATIYVGTDTNIDPSNPQSYQFYKQFAIRSSEYIGPDVNSEGE
                     QLINLTLEQEEIKVFQNPEVYLLPSFSFEATDGPVTIYASPADYIHMKGMLTVRIHIE
                     EE"
     gene            complement(45518..46021)
                     /locus_tag="PK015_01620"
     CDS             complement(45518..46021)
                     /locus_tag="PK015_01620"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOL33206.1"
                     /translation="MFLTFCKIGLFSFGGGYAILAMIQQEVVTKNAWITSSEFADIVA
                     ISQMTPGPIAINAATFIGYRQGGILGSVACTLGVIFPSIILMLAITLTYIKLSKLPWF
                     QNIFQKLRFVTLGLIAAAMILIGKTAFPDFFSIIIFVAALFVSWRWKTNPIYLLLGAA
                     VIGMILG"
     gene            complement(46033..46581)
                     /locus_tag="PK015_01625"
     CDS             complement(46033..46581)
                     /locus_tag="PK015_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOL33207.1"
                     /translation="MSNSLFSLFLTFLKIGAFTIGGGYAMIPLIRREVVDKHHWLDDK
                     TFLDGLAASQSCPGPIAVNFSVYIGMHLKGFWGMIVAVLGTVLPSFVFIVIIAALFTQ
                     YAQLTLVQKAFHALKPAVVALIASPLLQMTRSSQIKPAFLWVPVLVMILVAFLNISPI
                     WIILTTIIFSVLQSLWQKNRSE"
     gene            complement(46574..47641)
                     /gene="ispG"
                     /locus_tag="PK015_01630"
                     /gene_synonym="gcpE"
     CDS             complement(46574..47641)
                     /gene="ispG"
                     /locus_tag="PK015_01630"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOL33208.1"
                     /translation="METIKRRPTRQIKLGNVLIGGGAPISVQSMLSVPTSDISAALSQ
                     IHSLEKVGCEIIRFSVETMADIPSIYEFKKETHIPLVADIHFDYKLALAALQAGVDGL
                     RINPGNIGSRDKVEAVAKSAKERGVPIRIGVNSGSLPKELLAKYGLSSEAMVEAALSH
                     IRILEDIGFEAIKVSVKASQVPLMLASYRALSQKIDYPLHLGVTEAGTLLSGSVKSAL
                     ALGILLEEGIGDTIRVSLTADPVKEVMVGKQILQNLGLRKGLQIISCPTCGRTHINLM
                     RLAEEVESALQEYANLPLTIAVMGCAVNGPGEAREADFGIAGGVGEGLIFAKGKIIKK
                     VPEDRLVAELIQLIKNWKESV"
     gene            47768..49114
                     /locus_tag="PK015_01635"
     CDS             47768..49114
                     /locus_tag="PK015_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HOL33209.1"
                     /translation="MHKQILIMALMLLLAFFVQAEDWLTIYNDDLSLVRSNFELELKQ
                     GRQDYNYTDITSRIEAPSVIVLSQDGAVRVAEQNYEYDLAGKAQILNKYIDKKVDLFT
                     KDNSHLTGILKFYDGISFGLIEEGTNRLIVADNDQVQLIQLAELPPNFYTRPTLHWNL
                     LANKAGKHQLQLMYLTGGLSWDVTYNTVWDGHILALNSWVTITNNSGKAFNNVHLKLI
                     AGDVNRVRKGYYDTAGRGYEVMLKTADQAAPSFEEKAFHDFHMYTLDQPVTFADKQTK
                     QLELYPLQNITASQFYEYNTFASSVLSIIKFKNTEANGIGKPLPKGTIKVYKKDVDNN
                     LEFIGEDSINHTSKNEEVRINTGSAFDLVGKTIVKDRKTISQYISQRTIQVTLRNNSN
                     ENKSIDVIHNLGTNAKIISVENVTQPTVDEYNNATFKINIAPNQEIIFSFVERTEY"
     gene            49144..49584
                     /locus_tag="PK015_01640"
     CDS             49144..49584
                     /locus_tag="PK015_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOL33210.1"
                     /translation="MQERCYDNLRSLRREMLKFLSDASLRSRDPLTMENAIDDIWHPK
                     CDVFQTDSQWIILVELAGMEKEDISISRGDDYIKISGERDLHPEFEHATYYSMEIETG
                     RFERIIYFPDLNLDKDNPQVQYKDGILQIAFNIEATIERIIPIE"
     gene            49700..50587
                     /locus_tag="PK015_01645"
     CDS             49700..50587
                     /locus_tag="PK015_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33211.1"
                     /translation="MIAGICFLSAETRILSIRSEDLSSTSNKIYITVSDSIRYSVQKL
                     TEAKGVRVILYDVEGNVKPSYPRLSAVLDRITCYPAGNNLVVDIITMGDYPVNSYFES
                     GENLLTLTIHSSGAGKAMSSKVLGKAEKTLKSEAVSQTVNKEKIKTSSGNTNNEFGLS
                     SNKIEDIPSQESVADSLQLSSSDTISIPVEEVGNEPNCNLPSFWNRYKYLILSIITAV
                     CLFFVLKSLFKKKAKRPGNEDKSGEESALLLPPETAKLMVQKLLDKDWKPEEIARELH
                     LSVKEVNKIISALKKESDE"
     gene            50580..52112
                     /locus_tag="PK015_01650"
     CDS             50580..52112
                     /locus_tag="PK015_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33212.1"
                     /translation="MNRIPAGKFIFVFVIFLAGISFCCAYSFWEDSDLIPEVRTEVYN
                     SILSINDTYHSDPHTINRLGVNHPFQTLRKFRRCAREAFLPGEKSHSFVQYAYADSEL
                     YYSMDINFTAGYDQRSDDPGDYGFFYKGLRFNSQCNDRFRVRGNWWNGAFHGSRMYWE
                     HSPLIDGYNNQNPDKNLLDNMNGDISYNNKYFNAAVGRGRFQIGNSVSGSMILSDKVN
                     EYDYLLLEQRVGKFSFSLLNSILKADSTKTPDSDDYPDKFFALHQMMYKPLDKLELFV
                     GETVVYGSHYDLSYMLPFYFWRVGKYGMQEKDNLMIYGGANYYPAPNLVVYLNVAIDE
                     LTYNKIFTDWWGNKYGIQTGVSMKIPEFALTDNESPRLTVEATAIRPWTYTHYDNVTM
                     YSNERLPLGYPKGSNLVDLTAELNLPLPWKMRWDSQFSCTWQGSVGNDWRLNYHTYFP
                     SDIVNTAEAYWLEGDVSFIPTWTNAIHLDFMAHHSFILGHSSTFSKDSRHQLFLSWQF
                     FY"
     gene            52115..52534
                     /locus_tag="PK015_01655"
     CDS             52115..52534
                     /locus_tag="PK015_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33213.1"
                     /translation="MPIVDRLEYPKKYKMSSAILLLAVLLAVGALLYQKYSNKAMAKQ
                     IVIHSIGFDEYDKNYIRVGYEIENMGKKDQEVALLIRVYDREGGEIASIFFKTNIKAH
                     TREFQSKVIDELNRPLKEGEKPYRATIELRQREFMHY"
     gene            52643..52933
                     /locus_tag="PK015_01660"
     CDS             52643..52933
                     /locus_tag="PK015_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOL33214.1"
                     /translation="MTKADLVKIISESTGIIRKDVAVVVDAFLQAIKDSLSQGNHIEI
                     RGFGTFKLKTRKPRVGRNPKTAQRVPVPARTVPTFKFSREFKNSVSNVKIDE"
     gene            53086..54234
                     /locus_tag="PK015_01665"
     CDS             53086..54234
                     /locus_tag="PK015_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOL33215.1"
                     /translation="MDNEFWEVVAEELKRLLQGDFSSHKDFPYFYTKYSYPIASPLDG
                     LPILPQTIPEVLLKWAQMDSESQWNPEDFLFLDLETTGLGRNQTFAFLIGLGYYEKDQ
                     LVVEQIFLPVPEAEVNSFDRLAELLSRKAVLITFNGKTFDIPILESRLLYNRIWLNLR
                     EKEHIDLLQLARRLWKNKLPSCALETLEFYLMGKIREEEWDIDSELIPQTYFQYLQTG
                     EPEALKKVFIHNQLDILHTAALFTLICDSIDYPPVAGYDQRIDYYALAQLYLRQGDKD
                     KAKYILSDLLNQNYLTGDVACALGILCKQEGNIIGAERYFAIATDLFHPDGMLEYAKL
                     LERRKAYAQALEVALKLLHWNLSRPLVNSKRILELEKRVKRLQRKIGD"
     gene            complement(54842..56266)
                     /locus_tag="PK015_01670"
     CDS             complement(54842..56266)
                     /locus_tag="PK015_01670"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOL33216.1"
                     /translation="MQKFILMVIALLLCLTIYAQSSYIDELLFAAKNGDTDAQYELGR
                     IYYEGKLVPRNLYEAVKWLALAADNGNSAAQCDLGWCYFKGEGIEKNAVEAARWWRIA
                     ARQGNPDAQYLYGWCLDNGYGVAENKTEAAQWYQKAANKGNSYAQNNLGFKYFAGEGV
                     EQNYYLAVSWWLNAAEQGNSDAMYNLAVCYENGLGVPKNQQEAEKWYRIAQEQEKKTK
                     GNLIVINQLKKSGIYYPSLESVTTPSTLTTELKSEKAKTSDESIEKAVSPAPISPKKP
                     EAIEPQKPATPAPKVPIYPTTAEEMYEWGMKYYQGEGVPQDYKEAVRWWLMAAEKGDA
                     RAQYSLGICYKNGRGVEKDDKEAIRWYMLAAEQGEPRAQYNLGVCYKFGIGVPKDYDE
                     AVKWYRLAAQQGHPQAQCNLGVCYANGEGVAKDLEEAYFWFSLAEVNGNKTAAENRAL
                     LENELTEEQKERIKERVQKWIINR"
BASE COUNT        17923 a        10196 c        10533 g        17703 t
ORIGIN      
        1 cgaggttcct gatcctaaaa ctacaataga aaccatttga ggagtggctt caacagtatt
       61 agagggagct gattctcccg ctggattaat gtaaacagtt gttacataat aagaataggt
      121 agtgccatta actaaacctg tatcctgata agtggtttcg gtaacatcct gaatcgggac
      181 attgttccga taaactctat aacagctaat caatctttct ttagtattat ctcttggact
      241 gttacgacct tctgcaggat tccagtttag agtcacagtt gcatttcctg ccaaagcagt
      301 aagattggta ggaggataag catttggtat aacagtgata gtagaagtgg gagaggattc
      361 tccattggaa tagtgcgcaa tgatataata agaataggag actccattag taacggagaa
      421 atcggtatag gttaaagagg ttacatttgc aatagaggat gagtttctaa atatcttata
      481 agcagttgga ttaccagaca ctggagcaga ccaggaaaga tatacagatt gatgactggg
      541 gatagcagtg aaattttgag gaggaagtaa ggattcaact tgtgggaaaa ttttaagtct
      601 taaattagga aggttgttag ttgcacttaa attcgtagga gcttcattat catttctatt
      661 ttgtcttgct ctgctggtac tggtagaagt ataagtccac ataggataat ttgatatcca
      721 ttgttgatgt cctttaacaa ttaagataga aagattggaa gttccatcat agacaaacat
      781 aggagaaaga gtgacttcca tccagccaga ggtggaatta ttagggaagg aaccactata
      841 aacttgagtg tacccacttg tgctataagt tccagaactc aaagtggaag cagtggtatt
      901 tttcatatat atagtaacgg attcaatagg acttacatct gctccgctgg ctttgtagta
      961 ggcaagattt ttaatggtgc aggcagagcc aatttcactg gctaaataaa ttgcctcatg
     1021 agcacaataa ttataatatc tatccagagg ataaggctga gttcccgttc ccgttccaat
     1081 agttaatacg ataggtgctc caacttccac agtttcagaa tgttgagcag tagtggttcc
     1141 agcgacagca ttaaaaataa ggttaattaa agttccagta ctcatactgg aagcagcact
     1201 aatagtaaaa gataaaggtt caaaagctgc tggttcaagg accgggaagg tatccgtccc
     1261 attaataatt gttattccag cattattaca ggttaaagtt acagttccag gaggggcagg
     1321 aattacacct gtatttacta tatgaatatt aactgtgaca gtttcacctg ggtctaaaat
     1381 accgttacta tttccgccac tatcattgat agtcatatta ttaaaaccga gttctggaac
     1441 tactacttcc agcgggaaat tatagttcca gttaccagta tcagaagtaa tatatatatt
     1501 aaaattgatt tgatgatgtg ctggacaatt aggagatacg aatatagaat aattagtttg
     1561 aggatagcca ctactctgag ctggtattgt tccataatta gcactggaat taaggatagt
     1621 tacataagaa tcagaactgg aaataactgc agagacccca gtagcattaa ccaagccacg
     1681 atttcccaga cgaatagata gatccgcatt ttctcctggt tctaaatggg cattatcgtt
     1741 aggatctatc acaaatatct gttctaaaac taaataagga taatctggta aaattggtct
     1801 ggtagtaata aaaagtgcgg aattatgaga aagtggagct gctgcagtag gataagtgtt
     1861 attaaaagta tattccaagc ctatagtttc ggtatggtct tcaattccga tagtacaata
     1921 atttccatga ggtaaagtat caccagaacc taaatcaata ttattgaatt gttggtattg
     1981 cagtttaatc tgtccatcta aggtgtaggt aggataatag atggggtcat ataagatggc
     2041 ttcaaaggtt tccagtgagc tattattata tccggaaatc atatgatacc attccactac
     2101 aaaatagtgt aaagttgcat tgtaataagt gtagacacca ctaccagttc caagcgtgag
     2161 gtcatcccag aaaacggcaa tcataggact tggacctccc gcatcaggaa ttctccagtt
     2221 acgccaatct gcatcctgag tttgtccaaa ggcaataaaa ccattagaac tgatagaagc
     2281 ttgagtgtat tctatcccat aaaatttaaa ggggaaggga agattaacag ttgttatagc
     2341 tacagctgaa gtttgatctc cttcatcact cgtatttcct ggatcataga gaggaagaag
     2401 agttccactg cctccttccg caggtgcaat accaatccag ttataagttg gacactgatc
     2461 ataccctgtg tcaccctgat caaagataaa gtatccataa gcatcctgtc ccaatggatc
     2521 gtgtattgta gtttgtccta tggtgacagt aaaataaata gtttccttat aacccgcaga
     2581 gttatagaga taaagttcca gaggtatcac cattccgtct atacaggtac tgcgggcaaa
     2641 aacactaaag gtatcagaat tattagaaac agttgaggca acaggaatag acccataagt
     2701 tccaatagaa tcggttattg taaagtaaat atcacgagaa cgtagtcttg catttattcc
     2761 atttaaccca agtgaaccaa catttgttag gctaatagtc ataggaaggt tatcaccagg
     2821 atgaatatag ttattagtgg cgccaataaa agtataagag gctatattta gattaccatt
     2881 atgaactgta aagggaacat acacttccca gctacctgcc gtagtttcca ctgtaagttt
     2941 tagggaaaat tgatgttgat caggacaatg tgaagcgaca ctaaaaacta tggggtcaat
     3001 attcacacca cttgcttgag gatttatatt attatattga atacggctag caagaattaa
     3061 agttacatac ggatcagaac aatttgcaat tacagcaggt gtcaatactt gattggttgt
     3121 atttttaaga cttacatatg tttcaactgt ttctccagca tctaacaagc tattaccatt
     3181 accccacata ttgtcatcaa gttctgtacc attgaaaaca attccaccac tgctgacaat
     3241 attaatggtt gataccagtg gtttgaaatc atctttgttg atagttaagg ttaaagagcc
     3301 agagagattg gaagcaaatt ccagataggc tttaccttga gcattggtat aagctaaagc
     3361 ttgctgtcca ttttcattgc tcaatacgac agaggctcct tgcacaattt gatcgtggtc
     3421 atttaaaact gtgatttcta aggtagatga acctgcaggg atggaagtag gtgcattgga
     3481 aatgaaagtt gaaggaatgc ctacataaac agaagcagta ggatcaccga ttaaattaca
     3541 ataggaagag aaattataag cctgggtgga ttggctagct ccatagacag catacagata
     3601 gagtttagca tagagcattg cttcgctcat atttcgcatt cccagaggat agatgccatg
     3661 aaagattcct acagtcagac agttgttcat aggcgtatga gttgaggaag ttgccattcc
     3721 tattgaggtt attgcccctc ctaaagaagc tgcagttcca tatctaacta ccgcttcagt
     3781 ggtactcgtt ccactgttga aagtaccggt attacaagta ataaaaacac tatgnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccatat atcaccagca ttctgggacg
     3961 agaataagtt atttcatcac gggtaataaa ctgttcgtaa ttcaaaatca gagcaggata
     4021 aatcttttca aatgcaggag ataagtttaa ggggggttct atttcattga tagaaggttg
     4081 attatcataa tttaattgaa tatcaattga ttctgagatg gataaagttt gtttactctg
     4141 gtcatactgg aagggataga tatttaaagt gacaatacga aaatcacgca taatttgagg
     4201 ttctgaaatt actactgaat ttgcaggata caattcttca ttatatcttc cgcttgcttt
     4261 ctcagaacta agtaatgagt caaaatcaag tctaatatga ttctgaatac tttgttgagt
     4321 agtgtttatt tgcagattga cccctccctg atagggaatg gcaatcatcg tgctaaatac
     4381 tggaagagtt tcggtctctt ccaggaaaag ataggggata tcgtctattt taactcggga
     4441 tagattatta atttcacttc tttccataga ccaggctgga agttcaaaga gtatttgaat
     4501 agaattgtcc gaaatattaa caatattaaa aggagcaacg gaggtttcag ccagaagaaa
     4561 tagtggagct aacagtataa gtatcagaga taaatattta aaagtttttt tgggcattag
     4621 ttatttcctt taatcatcag gaattaatat ctttatagat atatgcaaaa actggtgcaa
     4681 aaaataaata ttttttatag attacagtga tacaggataa ctttaaggag acttgcctat
     4741 ctctgcatct caaaaagaga ttgtctttca gatttattag aataagattt taatataaaa
     4801 aggagtgaaa aaaatgagac aacagttgaa tcttggtatt gacggtatgc attgtgcagc
     4861 ctgtagtgca cgagtagaaa aagctctctc cagtttaaaa ggagtgattt cagcaagtgt
     4921 taatcttgga ctggaagaag cttcccttga atatgatgat agatatctaa aggttgaaga
     4981 tatagtaaaa accatagaat ctctgggcta ttcagttcgg gaaagtatta tcctgacgga
     5041 agatgaacag atacagaaag tgcaaatagc cagaaagaat atgattcaga tgtggattat
     5101 agccatcatt gtgatggcaa taatgttaat aatgatggca tctcacgaga tgccttcctg
     5161 gcataaggca gcaaactgga taatattttc tttaactatt ttagggatgt gttttcctgc
     5221 tcgtaaagtc tatatttcag ctttcaaggc aataagcact ggaggcacca atatggatgt
     5281 attgatagct attggcacca ttgcttcatt gctggtagtt cctttatctt taatcgtaaa
     5341 tggaatacga gcagatgatt ttagtggaat cgcagcaatg attctggcat ttcatttaac
     5401 gggtagatat ctggagacaa aagcacgagg tcaggcttct tcagcaatta gaaaacttat
     5461 taatctggga gcaaaaacag ctcttgtgct tatagatgat gaagaaaaag agattccagt
     5521 gcataaactt aaagtaggag atatattttt agtaaaagca ggagcaaaag ttccagccga
     5581 tggaattatt attgagggta gtgccaatct tgatgaatca atcgctacgg gagaatcctt
     5641 acctgtatat cgtcaagcag gagatagagt tctgggagca actttaaatc ttgatggctt
     5701 tattaaagtg caagcagaaa gagtaggtaa agatagtttt ctggctcaag tagtaaaatt
     5761 ggtggaggag gctcagaatt ccaaagttcc agttcagcta ttagcagata agataatatc
     5821 tgtatttgtt ccagttgttt tgacactatc cattttaacc ttttttgcct ggctactttt
     5881 tccgtctttt atgagtaata taagcgaagc cattcggaca attattccat taagttcagc
     5941 taattatggc ttagctgcag ctttgatggc ggcaattgcc actctggtga ttgcctgtcc
     6001 ctgtgctctg ggacttgcta cacctacagc tttattggtg ggtagtggaa ttggagctga
     6061 aagagggatt ctaataagaa atggagaagc tttacaaagg atgaaagatg taaaagttat
     6121 agtttttgat aagaccggca ccattaccta tgggaactta aagcttttaa agatagtgag
     6181 tccaaaactt tccgaacaag agatattaca gattgctttt agtctggaac aaggcagtga
     6241 acatcctctg gctaaagcta ttattacaga agctcaggtt cgtgacctgc aaacctttcc
     6301 tattgaagaa ttcactaact atagtggaaa gggtatctct gctaaaataa atagtcaaag
     6361 ctattatctg ggtaatcttg ctctcctaaa agaaaagggt attaaaatag acgaattcaa
     6421 cctaaaagat acacaatatc cttacgctac ttccgtattt ttgacggatg agaagcaagt
     6481 cctgggtata ctatatctgg cagatactat tcgtcctgaa gcttttgagg tagtaaaaac
     6541 tctaagagaa atgggaattg aaagcatttt attgagtggt gataatcaag agaccactgc
     6601 tgctattgca gcagaatgtg gtataagtaa atttatagcc gatgcacttc ctggagataa
     6661 agctaaaatt attaaagaat tacaagaaga atatggtgct ttagctatgg taggggatgg
     6721 tattaacgat gcgccagcac taaaacaagc agatattggt atagctcttg gtcagggagc
     6781 agatatagcc atagaagcag cagatattgt aatcgttccc aataaattag agctcattcc
     6841 ccgagcgata aagctttctc ttgctacttt ccagaagatt agacagaatc tgttctgggc
     6901 tttcttttac aatgttattg ccatcccctt agcgattttt ggtgcattac atcccattat
     6961 cgctgaaata gctatggcta ttagctcagt aacggtggtt agtaatgcta atctattgcg
     7021 tcgtgaatta cgccatctct aagtttagac tttaaactca gggtgtagtc tcatctatat
     7081 aatttatcag gcgagaaaga caattggtgt atgaaccgag ttcattatca taccagccaa
     7141 agattttagc atgagttaca ggcatataga cagttttatc ctctggagca cccaattctt
     7201 tcagggtctt aggtggcaca gcaataaagc ttgtacgggt gtgagtttca ttagcttcta
     7261 tcactactgc tgccatattg ccaatcatat ctgctgaaac attttgtctt tcggtataca
     7321 ttaataatcc attttgtgga ccttcggctg cctgtttata aatatcatta attagttttc
     7381 tggtgatatt gggttgtcct aattcattta gttctgcatg gaaggttaca ttaaggttaa
     7441 taagagatac agtgttagta ggaattcgca cagaatctgc cataaaacct atatttttaa
     7501 tttctggcat caccagttcc agagctttag aaacaccggt agtagaaagg atgatattat
     7561 tgaagacact gcgggtttta cgcaggtcag aagctcctgc tttaggcaca gaatccagaa
     7621 tgctttgaga attggtggca gcatgaatag tgctcataga tgcagtcatt atgttggctg
     7681 tttctttatg ttcaagtaga ggttttatca tatgagccag accagtggta gtgcaacttg
     7741 cagcggagat tatatgatgt ttttgagggt cgtaattgag gtgattgata ccataaatca
     7801 ttgttatggt atctggatta tcttccatta ctgctgcctt acttttgaaa ggagctgaac
     7861 aaatgacctt gagagcacct gctgctaaat gacctctaag acagggcact cctttttcag
     7921 gagtgatatt ggggtctaaa aaattacctg tacaatcaac tactatgcgg acaccttctt
     7981 ctaaccagtt gatatctttg ggatcacgtg ctttacgaag tatagttatg ggcataccct
     8041 caatctccag aacaggtttt tctgggtcta taaccttgat ttctgcctta cgtccaacta
     8101 ccccaaaaag gaatttgtga atgctgccat aggtggaatc catttctaag acctgaatca
     8161 ggtcatccaa gctttttcct acttctctgc caaagttaat gacaatacca tcaaaatgtc
     8221 tcagatgaat ctgattccag agcagaagtt ttcctattct tcccactccg ttgattccaa
     8281 gtagcctttt acctttcaat tggctgttca ttgttttctc cttatctata tttttttgcg
     8341 ttaatatctt aacctggtat ggtttccacc ggataatgtc ctaaatatat tgaaccactg
     8401 gaagcatcaa tcgctatctt atcgccagga cgcagaatat aatctcctat ccgacagata
     8461 ttgtcttctt ctataacctt gagttgacga cagttaacga cgcctataat tcccagacga
     8521 acggctgtta cagcagcatg agaagtgaca ccaccacgag aagttagcaa accctgacaa
     8581 tcaaataaaa gttccatatc ctcaggaact gtatccggtt taaccagaat taaaggtaaa
     8641 ttcatatctt ttagttcgtc aatatcttga cgagaaatga caattatccc attcaggaca
     8701 ccctttccga ctccaattcc attacctaag agtttcaatt cacgtttagg gacactgaaa
     8761 actgtatatt gtttaacctt agtaattagt tgattgcgtg tctgaagtat atataattgc
     8821 tctttgcgtg gaccctcaaa agtaaattct atctcttgat ggggatatcc acgttcttct
     8881 accaattgac gagccaggga aagtaaacga gcataaatgt ccgggaaatc tttttccagg
     8941 gaaacttgta ctggtgattt agataatttc cgttgctgtt ctgagatagg caaagtatat
     9001 accaatcctg ctactacatc ctctccttga ctgcacaagg taaagtcacc attcagagtt
     9061 attcctggtt ctttactcca atctgcataa gtgaatgcaa cacccgtgcc tgaatcaaga
     9121 ctgatattac ctaaaatcat tttttggata agaacagccg tgccccaatc atctgctatc
     9181 tgaagttttt gacgatataa acgtgctcta tcagtattcc aggagtctaa aacatgattt
     9241 atagcctgtt taagctgaag aaatacatcc tgctcaaagg aaaccttatg acgagctaaa
     9301 acctttttat aggaagtgca catatctctc atctgctccg gagtaaactg agtcttaagc
     9361 tgtactttat accgacgctt ataagaaatc atcaccgcat caaattcatc acggtctatt
     9421 ccatatgcca ttccccaact ttgcaaaaga cgacgataac aatcccaggc agtccaacca
     9481 aaattaggca tagtgcttag cttgagagta atctcatcat tcataccaat attgagaaag
     9541 gtgctcatag ctccaggtaa actcatagga gctccagaac gcactgatag taatagagga
     9601 gcctctggat tgccatattc caatccggta taattttcta tacgattaac attgaagcgg
     9661 attttttcat ccagctcttt ctctatctct ggatgcgcat taattatatt acgagctcgg
     9721 aatagctctg tagtaataac aattcctgcg ggaatgggaa attcatactg aatcattttt
     9781 ttaagaaaat aagccttagc acctaaaagc acttgattgt ccagacgacg gttatttgta
     9841 tgtagaggta caaacagcaa atcagggtca taactcatca ctttaatgag gtcctctgga
     9901 gcaaatatgg tcttcataga atttaaagaa gtgataatct gagagataaa attgtccatt
     9961 gattggacga gaaatgagct acccagaagt ttacgaaaaa attcttcagc aaacatttgc
    10021 tggatggttt gttttttata ctttcctggt attcccttag cgggtaaatg gcttagttct
    10081 tgttcataaa aacgatagaa atgctcattg attaaatcgg tgatactatc ttttatctgt
    10141 ttgaagaggt ccagatattg gtctaaggaa agtctgacta cacgttgact ggattttaac
    10201 atctccaagg cattggaaaa agcactgact gaatagcctt cataaatcaa tcccaattca
    10261 tatagctcaa gaatatggat gatacgtttg agggttttag cagttagata ttccagattt
    10321 aattgcttta tgtctttttc aatcagtctt tccaccagat tttccagacg gaatattatc
    10381 cccatagctt ctaatttcgg ttctcggtat tgaccataca tagaaggaat cccagcagct
    10441 atgtgtcttt tataatatat attttcccag ggctgggtag gttttggatc aagcactatt
    10501 ttatttaatc tgccaatata attaagcatc tgatggatgc tgctatcata gtcatttctt
    10561 gccagactct gtttcaagcg aagctgctcc ttctggtcaa accagttata tcggcttaga
    10621 aactttgtta tatcataggg gtccagctga tatttatcca gtaaaagata ataaatccgg
    10681 catagcagtt ctaatttttt aagtgggata tcttcatttt ttattgattt aaaaagtgaa
    10741 gcaatgcttt gccaggattg gttgagcagg ttttgtgcat ctaagttgtg gtcgcttaaa
    10801 aacttattta ctgcctgatg ctgtttctga ttgagttcat caggatgact tatatattct
    10861 tctatatctt caggtgccag acccttcaaa tgattagggt caaggtcaac ccagtaattt
    10921 aaaatctctt ggagcagctg aatctgagta ttattacttt cagtgtgcac ctgtttgcgg
    10981 acaaagtgca tcaataaatc cttacgatag cagagctcat caatttcggt gcttaagtct
    11041 ctaatttcac cttctgctcc tatttcatta taaaaaacag gtaaaagtct taaaagatgc
    11101 tttgcttgca catagatagg tttgatgttg ctatttagaa atgcagatac atctttctgg
    11161 aacaaatccg tatcggatac aaagattccc tgtcggatta tagaaatagt taaagcggct
    11221 aataaatcct tacaaaaagc tgggtcaatg ctgataagtt gaagccagac acgtatattt
    11281 ttaatgtggt ttttattggc tataatctgc cagtcccggt taattttaat ttttccgggc
    11341 ggaataaaac caagatctat tatctttctt attaaccttt ttcccagttc gggttcttca
    11401 gggtcaagta ctgccttgcc aatagtgaga acacaatcca gcacgatact ggtatgcaca
    11461 tccttatatt cagccaggaa ttcaaaaaca gaatctaata gcgcatttat ctctttagca
    11521 gataaggtct ctcgtaactg tcctaaaatc cgattaaaat cccataaaag atggtcaatt
    11581 aaaccactca tagggggtaa actcaaaaga taaaagatat aatgaatctt ttcctgtata
    11641 cttggaaatt gagccagcaa atctctatga cgaacggcta tttctgtaaa agaaggaatc
    11701 tcgtttaatt cctctatact tttaatctta cccaattggt ttaattgctg gttatagaac
    11761 ctggtgccta ttgtacggga aagataactg cgactgagag taatcaaatc agcatattta
    11821 tcataccatt cctcaattga agagctggat ttccacaatt ccagagaaga ttgatagata
    11881 gattttaata gcacaagtaa cctttctgga tgggatatcc tggtggaaag aagcttcaat
    11941 gtgctataac attgtcctgt aagcggaata aataaatccg ggtaatctct ataccatttt
    12001 tctaatattt ctccacttgc ttccagaatt atttgcggaa cctgttcctg acttgctaaa
    12061 gcgctaacga aggatagaaa ttcagttata accttctgct tggtttcttt tttttctgct
    12121 ttttgaaaga tttcttcaaa gattcttaga ataatagata gagccttatc tctttctgga
    12181 agctgaagat agaaccataa atctccaatt acactttgtc ttaacaaaaa tagcacctca
    12241 tcccaattgg cataaggatg atgaaattct tccaaaaatt gcttggttct ttgttgtata
    12301 ccgaaataat tggtactttg ctttagtaac cactggctct ctggtgaaag aacaatctct
    12361 tttacctgtg tttgagccag gttttcagct aaagcttgag attgtatttt atccactatt
    12421 atcctcaaat attctttatg attttaaata ttgattataa tgatgttccc taaaaagcta
    12481 aatttcaatg ctcttaattc tatagatatt ttacaggtta taatgtcaag cttcttttca
    12541 gtgcgcatta aaccagctat gaccgtgtcc aatatcaact tttaaagtca caatgcgact
    12601 gtatttttcc ggtaaagctt cctccattgc tttcttaacc aacttctctg cttctaacaa
    12661 aaattcttta tgtacttcaa agacgagttc atcatggact tgtagtatca tcttgattct
    12721 gggattatct ttaatttgtt tatgtatatc aatcatagca atcttaatga ggtcagctgc
    12781 agtaccctga ataggcatat ttacagccac tctttctgcc tcagctctta atccctgatt
    12841 actgctattt atatttttaa gatagagttt cctgccaaat agggtctgac aaaatccatg
    12901 taatcttgcc tctgctttgc aattattaat gaaagcttgg atggtgggaa aacgatggaa
    12961 atactcatca atgagtttct tcgcttcctc ttgagaaatt cccagttccc gtgaaagttt
    13021 cttctgtccc attccgtaga gtaaaccaaa atttataact tttgctcttc ttcgttcttc
    13081 atcagtgaca tcgtttaagg gttttcctgt aattagagca gcagtttgac gatggatatc
    13141 taagtcctgt ttaaaagaat ctatcaggac ctggtctcca gacattaatg ctaacaatct
    13201 caattctatc tgggagtaat ctgcatccat aagttcccaa tccgggtcgg tggcaatgaa
    13261 agctttccga accgtcttac ctaaatctgt tctaactggt atattttgga gatttggatt
    13321 ggaagaggaa agtcttcccg ttgaggttac ggtctgatta aaagaagagt gtattttccc
    13381 ggttctcgga ttaattaatt ttggtaaagc actcacataa gtggattcaa gtttacttaa
    13441 cattcggtat tgcagaagtt tctcagcaat ctcatatttt tctgccagct cttccagaac
    13501 tgcactatcg gtggaataac cacttttggt cttcttctta gcaggtaact tcttctcttc
    13561 aaaaagaacc tttgccagtt gctgagttga attaatgtta aactcataac cggcataggc
    13621 atatatctct tcggtaagtt tttccagttc ctggttgata tttttggata tctctttcaa
    13681 tacttctgta tcaatgctga ctccattttc ttccatctgc tgtaaaactg gtattaaagg
    13741 gatttctata ttttcatata gctcttttaa acctgtgttt tccagccttt tcagatagat
    13801 aggatagagc atatagactg cccaggcatc ttccgcagaa tagtcacaag ctttttctac
    13861 ttccagtaaa tcaaagctgg ggcaggcttt tttattaatt aaacttgaaa aagtaatcat
    13921 ctcatactga agttccgtag cagtacaggt ttccagagaa tattgatttg tcccagggtc
    13981 taaaagataa gcggcaatca tggtgtctcc aattttatta tcccagttcc agccttcctg
    14041 gtggagtaca atcatatcat acttaatatt atgacccaga atcaatttac cttgcaaagc
    14101 ttcatttagt tcacggagag tttgctcttt agggaaatta ttatactcct tgtgtcctat
    14161 aggtatatac caggcttcat cagatttaaa acataaggaa ataccaacta aatcggcttg
    14221 cattgcatct tcagaagtag tctctgtatc aagacttacc agattaacct tcttcaaatc
    14281 gtccagcagc ttagaataat tttctgctgt tgccagtatt gcctgaaact tcttatttcc
    14341 cattttggag ggaactgtat acttactttc aaatatatca ctctgtacca atttatccgg
    14401 ttcttctgaa ggaattgaaa tagctatttt ctgcccataa cgattatcta ttaatctctt
    14461 caaagtattt atctcatagc gagtgagaaa atcctttgct ttatgtaatg attctggatt
    14521 aaattttaga gcttcaattc gggggatctc aataggaaca tttctgatta tcatagccaa
    14581 ctgtctggaa agataggcat tatctttacc ttcaatcagc ttttgtcgta atttttccgg
    14641 aatttcatcc agatgagcat aaatctcatc caaattggaa tattgcttta gtaacttaac
    14701 ggctgtaact tctccaatgc ctctaactcc gggtatatta tcactggggt ctcctgctaa
    14761 tgccagaaaa tccacaaatt gttcagggag tattccatat tttttaacaa ttgcttcttt
    14821 atccagagta atattcttca ttgggtcgta gataactgcc ttatcgtcaa ccagttgtgc
    14881 ataatcttta tcaatagtaa caaacactat atcataatca gttttaaagc gttctgctaa
    14941 agttgccaga acatcatcag cttcataacc gtctatggat atttctttta gaccgattat
    15001 ccgaaaaaac tcttgaactg gttctacctg tgccagcaga gcatctggca taggaggtct
    15061 attagcttta taggcttcac tgagttcatg tctaaaagtg ggtgctttac ggtcaaagct
    15121 tatcaataaa ttttctgcct ttttggaatc cagtaaatgc aagaaggaat tgaccacacc
    15181 atatatagca ctggtatttt cacctttact atttatcagt ggattattaa cgaaagcgta
    15241 ataagagcga tatagaagtg ccgtaccatc tatcaagtaa agtttatctc gcattatctt
    15301 tgctcctttc ttaaagttat tttctttttt cttattctct tatcctgatt aaactgtcaa
    15361 tattaaatca cttatattaa aatacatatg gataaaaaaa ggatttgcag cttaatcttg
    15421 acaaaataca taaccataaa tatttattaa agattgaaat ttaagattat ccaaggagaa
    15481 ataatggctc tggtcttaaa aactactaaa ttcgtagaag cccagattga tacctttctg
    15541 gatatagtca gtgattctgc tttaattttc cgttttgcag tggatgacta tctccaggaa
    15601 aggacttcgc aatttgaaga aagattgaat ctgattagag agaatgaaaa gaaggtggat
    15661 gacctcagga tagcagtaga acgctattta tatgaacgaa cactaattcc agaaaatcgg
    15721 ggtgatgtcc tggctatact ggaaaatact gatgaggtta tggaccatat gaaggatact
    15781 ttgatgcagt tttctataga aaatccggaa attccttccg tactggatga cctctggctg
    15841 cagactacaa ctgcttctac agctgctgta gaacaactgg tctttgctgt gcgttccttc
    15901 ttccgtgacc tcagtcaggt caataacttc atccataagg tctatttctt tgaaagagaa
    15961 gctgaccaat taggagaaaa actacgccgt caaatctttt ctctggatat agaccttgcc
    16021 cttaaaagtc agttacgctg gtttgccatt catattgaac agatttctga ttatgctcaa
    16081 gcagtctgtg ataggcttgc tatctatact ataaaaagac aattatagat tatcagctga
    16141 tatggtctat ttctatcttc tgagtgggtt attcatcggt tggtcaatgg gagccaatga
    16201 taccggtaat atttttggtg ctgccgtttc cacccgtatg attaaattcc gtcaagctgc
    16261 cctgattgca gctattttct ataccttagg tgcttttctg gaaggaagtg gaccttctgg
    16321 aactctcgga agattgggtt ccatagatgc tattgggggc gcctttactg tcgcattagc
    16381 tgctgcttta gctattgtgg caattgtccg tttaggcatt ccagttgccg tttctcaaac
    16441 tattgtggga gctataattg gttggaacta cttttctgga cgcatcaccg attttaattc
    16501 tctgattact attgccagta gctggattat tgcatttatt gtctctgctg cagttgctgc
    16561 tttattcttt tatcttatgc gaaggtggat aaatcgttcc cgactacatc ttctggaaca
    16621 ggatatgatt atccgttatg ctttagttgt atttggagct attgcagctt atttcttagg
    16681 tgcaaacacc attgctaatg ccattggagt ttttgtacca gttactcctt ttaaagattt
    16741 aaccataggt tctatgtttt cacttactgg agttcaacaa ttatacctgg ttggtgcgct
    16801 ttctgtagtt ttaggcatct acacctattc acataaagtg atgggaactg tagggcatga
    16861 aatctataaa ctttctccag ctatggcatt agtagctctt ttagctgaga cctttgttct
    16921 attcttattt tcttctcgtg gattgcataa tttacttctt tctatcgggt taccagctgt
    16981 tcccttagtt cctatatctt ccacccaagc tatggtcggt gctgtacttg gtataggtct
    17041 ggctaaggga ggaaaaaata taaaatataa tgtgcttggg aaggtatctc ttgcgtggat
    17101 tgttgcacct ttgatggctt tccttctttc ctttgttgct ctttatataa gtcaaaatgt
    17161 gtttgaacta caggtgcgcc ttcctataac ctacacagtg gataaagctg cagtaaaaga
    17221 aatagaacaa cgcggaatag atactaaaca tctatcattt gttaacctac gaaccttcaa
    17281 aacagaaaga gagctctata aagaattaat ggaagaagat ttctatacac ctgaagaggt
    17341 aaaaattatt attagtatat gtgaaaatta tcctctcctg gttaatgtgg aactcctcaa
    17401 taaaggaggt cttaatcgtc gttttactga agaacaactc aaagctttat ctgaattgga
    17461 tggtaaaaaa tttaagcgaa aatggaattt agctcaagct ctatccgaat accctgcctg
    17521 gaaatttatt gaaccaccac aaacaagagc tgatgaagaa cataatgaag atttaaaatc
    17581 agacttagaa ataatttata aagtcttcta ttttccatcg gataacaata accgctaaag
    17641 aactcaataa gttatgaatt tcttccaggg acagaatctc acctttattt tgtgtttctt
    17701 tattggtttt atcctgaaaa agctgaaact cttacaaaaa gatgatgctc acatccttct
    17761 tcgctttgta ctgaatgtct gtatcccacc tctaatgatt ctggctattt atcgtgctac
    17821 cttagctctg gatttacttt tagtagcact ttgtgcaatc ctggtgatgg tgataattta
    17881 tttcatctct tctgccatag ggaaaaagct gaaaatggaa ccccaaacat tcggaagttt
    17941 cctggtagct actatgataa tgaacacagc ttttgctctg ccttacttta aaattatagg
    18001 aagtgaagaa ctggctcgtg cttccctttt tgatattgga aacactataa tgatttttac
    18061 ctttgcttat tatcaggcaa tcaaatatgg gacttctgct actaaagaaa aaatccagct
    18121 caaaaaattt ctcactcttc ctcctttata tgccattctt tttgccgttc tgattaaaat
    18181 gtcaaatctg caactatcca atacggttat ctctttcctg gaacttttag gtaatcctac
    18241 tggaattctg atgatggtag ctctcggatt agcctgtgaa ccaaagctaa aacatctcgg
    18301 gaaagctctg attgctgtag tattgagaat gggagtaggc ctaattaccg gttatctact
    18361 aaccctcatt tttaggttac atggaacagc tcaaatcgtg gttataatct gttcttcact
    18421 acctgcaggt tttaataccc ttatcttggc tgatagagaa aatatggacc gcgattttgc
    18481 tgcaaccgtg gtctctatct caacttttat tgctctattt actataccct tactgattaa
    18541 cttcttctat taaaatgggt tgtagaagga ctgatacgat atcatcctgg gtatttttta
    18601 taagatttaa attgttattg cttcagtaaa gaagattgta cctcgctcca cagacgaaga
    18661 aatctgctgt cacttttatc ccttgaaggg atttatatcc cctgtaatag aattttgaat
    18721 agaaaaaaga agctttatta gcccaaaaac cttgtatcaa agcttaggtc acagactatt
    18781 tttagcagat agaactggag actgctaatt ttttatttgc tttttctctc actttcatta
    18841 tattaatagt agaaatcaat aaatgaaaaa ggaggtttaa aatgaaacta gtaccatctc
    18901 gtagaacttc agagattaat ccaatgagca cgatgttgaa tatgtttgat gaatttttca
    18961 atcgtgcttt tgaggaagat tttgaagaag aaaatttccg ggctatgcca atggatatca
    19021 tagaaagaga taaagaatat gagattctgg ctaacctgcc tggattcaag aaagacaatg
    19081 taaaaataac ggttcatgat aatcagcttc tgattgaagc caatgttgaa gagacaaagg
    19141 aagaaaagaa aggaacaatc tatcgctgcg aacgctatag tggtaactat cgtcgcaact
    19201 tacttcttcc tgataatact gatgtctcta agatatcggc taaaatggaa gatggcgtgt
    19261 tgcatctgat tatcccgaag aaagaaccaa ctccgcagaa agagatagtc attcaataaa
    19321 ccagcctcac aaagaaaggg acgcaggtat tcaaagcttg cgtccttttc ttttttaatc
    19381 tcatttattg tctgtctggg gaaaactttt ttaagttgtc cttgacacaa tatagagcta
    19441 ttcaaattta gctttaaaat aaattagggg agaaacaaaa tgctggttcc tgatgattta
    19501 tactacacag aaacacacga atgggttaga ttagatgatg ggattgccac tgtaggcatc
    19561 accgattttg ctcaaaaaga attgggtgat attgtctatc tggaattgcc agagattgga
    19621 aataaagtct ctgcaggtga accttgtggc actattgaag cagttaaatc tgccgaagat
    19681 ctgattagtc ctgtcagtgg ggttatagaa gaaaaaaata tggaagctga agaatcccca
    19741 gaaatcatca ataaatcacc ctatgaagaa ggctggctat tccgggttca tctttctaat
    19801 cttgaagaat tggaagactt attaagtcct cgtgactata ctaaattaat tgaagcttta
    19861 tgaacggtga ttaaaaaaaa taagaatttt ctcatcatcc tctcttcacc ttctggaggt
    19921 gggaaaagca caattcttaa tgaagtgcgg aaaataagac cggacataga atattccgta
    19981 tcttatacta ccagacctcc tcgtggagat gaaaagaatg gagtgcacta tcattttatt
    20041 gatgaagaag agtttttgcg tcggcgagaa gaaggtgatt tcctggaaac cgcccaagtc
    20101 tttggtcact ggtatggcac ttctataagc tttattaaag accgtttagc tgcccagcat
    20161 catattattc ttgatattga tgtgcagggt gctgctcaaa ttagctctac cagtattccc
    20221 tatgttaaaa tatttattat gccaccatct atgaaaatat tacgagaaag actcattaaa
    20281 agaaacaccg attcactgcg ggaaattgag aaaagactgc ttgtcgccaa gcaggaaatt
    20341 aagtttattc cacagtatga ttatctcata gttaatgatg aactttctgt tgcggtggaa
    20401 gatgtgttgg cagttattcg cggtgaagaa aaccgcacta accgttatcc agagcctatt
    20461 aaagactttc tggaagagga gtaataatta tgtataatcc taaaattgac ctatttcttg
    20521 aaaaaactgg tatagactat ctattttgcc ttctggctaa tcttgaagct cagagattaa
    20581 gtcagcttcc tgcttatgtc agacaacatt tcccggataa aattcctgtt atggctatga
    20641 atcatgtcgc cgataatgaa gttcccgatt actttaaaga aatagaagaa gccacgctta
    20701 agatggctaa agaacactta ccctatcctg aagaagaagt ggaactggaa gaagagacct
    20761 acgacgattc cggtgaacat attgaagaat tagctgattc cactcatcgt ttctatagcg
    20821 aagaagaaga ggaagaagaa atagaagaaa cagaggaaga ggaagaagaa tcctacgagt
    20881 ttgaggatga cttggataac tttgaggatg aagaagaata accaggttct atgattcctc
    20941 gtgaattaat tcagcatctg gaactgctga aaaatctcgg tgttaaagaa atctttctgc
    21001 ctacttctcg taaagcagaa atcctgaaag aactaagtgt taaatattct acctgcacaa
    21061 agtgtaaact tcataccgga agaatcaaat ttgtatatgg agaaggtaat ccggacgctc
    21121 tggctatgct tattggtgaa ggtccgggag aacaagaaaa tctaactggt cgtccctttg
    21181 tcggacctgc gggtaatctt cttaccaaga tgcttaaggc aattaatctc tctcgggaag
    21241 atgtgtatat ctgtaatatc gtgaaatgca gacctcccaa taatcgcaat cctgaacctg
    21301 aagaaaggga agcctgttta ccctatctcc tggaacaaat tgaaattata caaccaaaat
    21361 taatccttat actcggattg gttgcggctc aaaccttgct taatacaaaa ctaagtatgg
    21421 aaaaattgcg tctgaccact catagcttta aagatattaa aacttttgta acttatcacc
    21481 cttctgcttt attaagaaat ccgaactgga aaaaaccagc ctggcaggac ttacagcgtt
    21541 ttcaggctga atatattaaa ctacttcaat gaatgctggt tctttatata aaaaacttct
    21601 actttccttt ttccttgtcc tttttctctc atctatttat gccgttattg ctatctatgg
    21661 tgattctcaa ggcaataatg atattcataa acagatagtt gaagctataa tcaaatatca
    21721 accagacatt actttccatc tgggagactt aactgctcag gggaaaaagc aggaagaata
    21781 tgatgagttc ttttcttgct gtaaacccct tacagatttg tgtcctctct atccagttaa
    21841 gggcaatcac gatgcttctt ctgaattatt cttgaaaaac ttccctttct taagccaaac
    21901 ttattacacg gtagaatatg atagcttgct ctttatcatt ctggatagca ctctggaact
    21961 ttcacctcat tccgaacaat ttaactggct aattgagaca ttcaattcta atcctttaaa
    22021 acctaagatt atcctgatgc accatcctat attcagcagt ggttatcata gtggaaatga
    22081 agattgggca ctttatcttc cggctctttt tgcttctaat aaggttattg ccgttttttc
    22141 tggacacgac cataattatg aacatttaca atggaaaaac ctcaactttt ttatctcagg
    22201 aggtgctgga ggttctcttc gtccttctct ttctcaacat ccctatagta aaatcttttg
    22261 ctcttcctat aattatctta ttcttaatcg ccagaaaaat tatttgattt gcactgctta
    22321 tagtttagat ggagatatta ttgataaagt agtcattttt ctggatttct gattaacttt
    22381 caagctttct taaaatgctt tcattataac taaaagaccc agaacttcag aaaataatct
    22441 ctttttatct tacttactgc aaagtaaatt tattagatac tttatccttt ttctctataa
    22501 cttaataata aggtggaaat tattcaatta cctgtctctt ccttagctct ttcttgggga
    22561 attcgctaag aaagaagcaa gatggactat acaactatta aaatgtcaaa cagaacactc
    22621 taatatatta aaaacttgtt agttatataa cacatcactt aacgaataca atgagaataa
    22681 ataatggata taagaaaact attaataatc cagaaatgcg ttttatatta atgaataaag
    22741 gaaagattgc atttcccatt tgataccact tctcatattt tcccttgaga ttatttagaa
    22801 caaatcttta aatcttatac tgcttctaat atttagattg acggaaaata atatctatgg
    22861 tttttggaaa tacaggatta agcaaaaaac ttgtttcatc agaatattaa atgtttaaat
    22921 atcaaataat aacaaacttt aaggagatgt acttatgtta caaggttcct ttgtggcttt
    22981 agtaactccg tttaagcacg gagaggtaga ttatgtagcc ttggagaggc taatagattt
    23041 tcatttagag aaggggacag atggtattgt cctcttaggg actacggcag aagcttccag
    23101 tctggcatca gatgaaagag aagcattgct tttatttgcg atgcagagga taaatcatcg
    23161 ggttccggta atcattggca ctggaagcaa taattttaac catactctgg cagataccaa
    23221 gcaggctcag gaattgggag cagattatgc gctgataata accccctatt atgtgaagcc
    23281 tactcagaaa ggtatggaag aatattttct ggctattgct gagaaaacta acattccgat
    23341 agttatttat aatgttccag gaaggacggg agtgaatatt tcatctacca cagtggtaaa
    23401 acttgcccgt caatgttctc agattgcggg aataaaggaa gcaagtggta atttaattca
    23461 ggcaacggaa atagttcgtg atgctcctga aggttttgct ctgttaagtg gtgaagatgc
    23521 aatgaacttt cctttaatgg caattggtgg caagggttgt atctcagtta cagcaaatat
    23581 tattccccaa gagttgagtg agcatataca tacttgttta aatggcgatt ttattactgc
    23641 tgcaaaacag catcagcatc tattaaagtt acatcaaact atgtttatag agaccaatcc
    23701 tattcccgtg aaggaagcat tgcatatgat gggaatgctg gaattggaat tccgtttacc
    23761 tatgtgtccc ttaatggata acaatagaga gatattacat aatgttttaa gtgaatataa
    23821 acttatttag gagaatataa tgaaaaagca ggtggaaaaa tacggtttta aactgcttga
    23881 gactcgtgag ataaaagaaa tctcagcaac tgcctattac tatgaacata agaagagtgg
    23941 agcccatttc ctcaatgtta aatgtgagga taataacaaa gttttctgcg taacttttaa
    24001 aactgtacct caagataata ctggatgtcc acatataatg gagcattctg ttttaaatgg
    24061 ctcaaagaat tttcctgcaa aatccacttt tatggagttg ataaaagggt cgttgcacac
    24121 tttcatcaat gcaatgacag attcggatat gactctttat cctgtagcca gcaccaacga
    24181 gaaggatttc tataatcttt ccagagtcta tatggacgca gtttttttcc ctaaaatcta
    24241 tgagcaacca catattcttc gtcaggaagg ctggcattat gatatcaaag accctgaaga
    24301 tgagcttaag attattggcg tggtctataa tgagatgaag ggagcatatt cttctccaga
    24361 taacttgatt gaattgaaca atcagattgc tcaatttccc gattcgcctt ataaatatga
    24421 ttctggggga aatcctaagg aaattcctca actcacttat gaagctttta tcaactttca
    24481 taaaaagcac taccatcctt ccaattccta tatctttctt tatggagatg ccgatgaaaa
    24541 tgaaataatg gaaatgatgg ataaggaata tctctcgcaa tttaataagg atgtggaacc
    24601 agtggaagta cccttccaga aaccttttac ccgaaccaag aaactggaag tggaatatcc
    24661 cgttgaagaa gggaaagata taagtgaaca atatcatcta agtttgaatt ttacttatgg
    24721 taaaaatcct gatttaataa ccgtctctgc cataaatgct ttgatggata tcctgatgag
    24781 tacacctgct tctcctttaa aaagagccat tttggaatca ggtttagctc atgatgtgag
    24841 agtttcttta aatatcggca ttttacagcc tactttcagt ttaatctgca aagaagttaa
    24901 aaaagagaat atccctcttc tggttcagct tatcaccgat gagttgaatc gtctggttaa
    24961 ggaagggatt gataaaaaac tcattgaagc agttctcaat cgtagagagt ttttcctaag
    25021 agaaggtccc agtggtaatt tccctaaggg cctttattat gctttcaata tttaccatca
    25081 ctggatacat ggtggtaatc ccatagacgc tctggcattt gaagatattc tgaaagaatt
    25141 aagaagaggt ctcactgagc cctattatga agaattgatt aagaaatcaa tgcttaagaa
    25201 tcatcatagc agccagataa cctatgttcc tgttccggga ctggctaata aatgggagca
    25261 agagataagg gaaaaactgg cggaagagaa aaacaaattg agtgaagctg tgctaaataa
    25321 gctgatagaa gagaccagag aatttcaaca atggcaacta caacaaccaa ctcctgaaga
    25381 gcttgcccga attccgttcc tcacgcttga agatataaat cctgaagcag aagcctatcc
    25441 actggaaata gaaaaactcg gaactaaaac tatccttaca catcctttaa aaactaatgg
    25501 aattgtctat ctcaaagctt attttgacct tgaccattct tccgaagaag accttccctg
    25561 gctttcgcta tatgcttctc ttatgggaat ggtgagtagt aaaaattatg attacataga
    25621 cctttccaac gaaatataca gtcatactgg tgatttgaat ttaaagttaa atatcagacc
    25681 cagttatcag gacccagatg tcattctgct caagttcctg cttagtggaa aagcactata
    25741 taataagaca gaaaaactta ttgaactggc aaaagaattt gctttctatc ctctttttga
    25801 tgactatgat agattgaaac tactcatccg ggaagcaaaa gccaaactcc aaaccagatt
    25861 attttatttt gccgaaagta tcgcaactaa ccgacttttt gctccttcca gccaattaca
    25921 tcattttctt gatcttacca gtggtctgga ttattatcgt tttctcagtg acttagataa
    25981 aaaattagaa caggatatag aaattattgc tcataaactt gaacagatac gggatactta
    26041 cttcttacag aataatctct taataagtat caccgcagaa gaagaagcca taaaagatac
    26101 cattaatgga ttaaatccat ttcttgatgc catttctgat aaagtgttgc cacctgttga
    26161 acgatatttt catcctaaag aactcaatga aggtattatt gctcccattc aggtgcagta
    26221 ctgtgccaaa ggtggtaatt tcttccgtaa aggttattct tattctggaa aattgagagt
    26281 actcactaat attctcaata gcgattacct gtatcgtgaa ctccgtgaaa aaggtggtgc
    26341 ttatggagcc tggtcagggt ttaccttgaa tggttatatg tattttgttt cctatagaga
    26401 ccccaatctt caaagaactc tggagattta tgatcagact tctgaattct tgcgtaattt
    26461 tgaatgcagt aagcgggata tggaaaggta tatcattggt gatatctcca atctggatta
    26521 tcctaaaact cctgaaggaa agggtgctca atcagatgat gattatatca ctggttttac
    26581 ccaggaagat agacaacaga ttagagatga ggtactttcc accagattgg aagatatccg
    26641 tcaatatgct gatatgattg aagaagtgat gagtatgaat cattactgcg tggtaggaaa
    26701 tgaggcaaaa ctaaaagcag ccagcgaact atttgataga ttaaccccac ttttatctta
    26761 aaactatgaa acccaaggac aagaaaatcc tcaaaccaca tcgtccagca aggttaaaaa
    26821 atacacgctt gctggacctt gaggtattgg atgaattgcc cgataatata aagtttaaag
    26881 caaaaagaac tcccgaaaaa gttgctgaaa cctacaaaaa agatatgcaa ttaatttcag
    26941 ggcgtatact ggaaattaaa agtaattatc aatatactat tgaagctgat aataaaacct
    27001 ttacggcaac tctttcggga agattaaagc aattcgcttt tcatagtaag tccatagtag
    27061 ccgtaggtga ttatgtggaa ctggatatcg tagctgaacc accaagaatt gaaaatatcc
    27121 ttccgcgtaa gaatacttta actcgttatc tgggttctaa tttccaaaga cctaccatct
    27181 tagctgctaa tattgaccag gtaatagtta cttcctcctg gcgaaaacca atgattaaac
    27241 caggattgat agaccgctat ctctgtcttg ctgctctgga taatattgat gctatcatca
    27301 ttatcaataa agttgacctc tgtgaagacc gggaaaaact ggaagaaacg gtatcttact
    27361 acaaaaatgt aggttatact cttctatata cctctgctct tacgggtgaa gggatagatg
    27421 aactttatga actgttaaaa aatcgcgata gtgtcttctc cggtcaatcc ggaacaggga
    27481 aaagttctat tattaacaga ctggaacctt cactgaatct tccaaccgct gaggtcagtg
    27541 attacaatga aaaaggaaaa cataccacta ccagcactat tctaattccc tggagttttg
    27601 gaggtcatct gctggataca cctggaatta aaacagtgaa tcttgctaaa gatgctaagt
    27661 ctcaaattcc ccgaatcttt ccaggttttg caactctggc acctttatgt tatttcaatg
    27721 attgcactca tactcatgaa gagcattgtg ctgtcctttc tgctttggaa gaagggaaga
    27781 ttcctactaa tcgttatcta tcctatgtta gtctattgaa ttccttataa tgaaaaactt
    27841 cgtaatctac atcaatcaag cttataaaga taaaactgct atttatgatt tgctggaaga
    27901 acttaaaagt gaggaccact tgaactttta tggcattcct tctcagcaag agattcttcc
    27961 tccagatatt catatcctgg aaagcgataa aactcctaaa atagattgtg tgctcgtatt
    28021 tggtggtgat ggaactattc tcagagctaa aaatatcgct cttaggacaa aagctcctat
    28081 tttaggaatt aatataggct atcttggttt tctttcggaa actaccttag gcgaattaaa
    28141 atcttctctt agagacctct tgaacaaaaa atatcgcttg caggaaagaa tgcttataca
    28201 atgtcagcta aggagaaatg gcgctattat ctataaagat ttggctttaa atgatgctgt
    28261 tgcctgtaaa gctgaaatgc caaaacttat tggagtacgc atctttaaca atagaagatt
    28321 cgtttttgac acacgatgtg atggtgtaat tgtcagcact cctacaggtt caactgctta
    28381 ttccctggct gctggaggtc caattttatc tccagaaatg aaagctatgg tattaagtcc
    28441 attaaatcct catcttttaa ctgttcgtcc tatggttttc ccttccactg accatatctt
    28501 attgcgtatc cataacctgg aagcaccagc tactcttcaa atagatggag ttaattgtag
    28561 tcccatagaa gaaaatgatg aagtagctat cactgctgca aagcagacag tacagttcgt
    28621 taaactttct aatcgcactt tttatcaaat tcttagacgc aagatgcact tgggaaaata
    28681 gatgctgacc gagctttata tccaaaacta tctctttgtt ccgcaagaaa gaatagaatt
    28741 tggaccaggg atgacagtct taacgggaga aactggagca gggaaatcca tattagttgg
    28801 tgctattgct ttaatttttg gggataactc ttccgcttta gaaccttatg atgaaaccaa
    28861 acctatctat ctggaagcta cctttgatat ttctcataat tcagaagtac aaagcttttt
    28921 acagcaacag gggcaggaga tagattctga acttaccctt gctcgggaaa ttaaaccagc
    28981 agggaaatcc acttattttc ttaatggccg caaggtatca gcctctcttt tgcgtgaatt
    29041 aaaacctcta atgattgatt ttcaccatca gcgtgaccag caaagattac tgtctaatgc
    29101 ctatcagcta caattattag acctttatgc ccaaaattcc tctcttaaaa aagaatatgc
    29161 tcaaatctat cactcgctga aatctgcctt gaaagagctg gaaaaactaa aagaagaaga
    29221 agaaaaaaat cgtcagctaa aggaactgta tcagtatcag tttagtgagc tggaaaaggc
    29281 taacctcaaa atgggagagg atgaggaatt gcagaaagaa tttgaactac tgagtaatgc
    29341 acaagaccta attgaaactg cttcttctgc ctatttcact ctttatgaaa gtgaagacag
    29401 tattcattct cgtttggctc aggttattaa tagccttagc tcctacagct ctatttatcc
    29461 aaggattgct cctattctgg aaaatttaaa ggattgtctg gaacaaattt ctgcttctgc
    29521 ttcagatttg aatgatatca gtcaaagtat aaatcttaat ccgcaaagac ttcaagaaat
    29581 tcaacttcgt ctggatgaga taaatagcct gctctataaa catcgtgcaa aaaatatcgc
    29641 tgaactgatt gaacttttcc agcaaagaga gggtcaaatc aatgcgatgg aagacctcag
    29701 tgcaactatt aaagctctgg agaatagcat tcagcaggat tatgaaaacc ttaaagagct
    29761 gggagaaaaa cttacaaaat cccgtcaaaa agctgcagat tccctggcta aagaactgca
    29821 agaaaatatc cgtcttcttt ctattcctga tgctctgttc aaaataaata ttgacaaaaa
    29881 gacaattgag aatatgttat tagaacaata tctaagtgta tgcagtgatg aaggagaaga
    29941 taactgtcaa tttctcttca gtgctaatcg gggaactgac ttaaaacctc tttctgcagt
    30001 cgcttcaggt ggcgaacttt cacgtatatt actggctatt aaaaaggtat tgtcggaaag
    30061 aatggaagaa aaacttatta ttctggatga gattgatgca ggaatcggtg gtaaaactgc
    30121 cgattatgtc gctcagttta tttatcagtt agcacagcat catcgcatta tttgtattac
    30181 acatctggct caaattgctg ctattgccaa tgaacaactg gctttgcaga aagtgccagc
    30241 taagcataaa aatgttatcc gtattatttc ccttgaacca caacaaagaa agcaagaact
    30301 tgccagaatg ttggctggtg atatttccga aatttcttta aaacatgctg aagaattaat
    30361 gactaaatat aaatgaggtt ataatggcaa aaatttccag tactgcccgc aagagaatta
    30421 aaggtatagc tataattatt atttttcttt cgctcggatg ggcaattttt tctgcaatgg
    30481 agcaaaagga tagtggtcct aatttaaatg aaatcttcaa aaagtcaaat aagacctttg
    30541 aagaaactca tgccattaaa aaagctaaaa ttagaatcag cagtcccaat atcgctaatg
    30601 ctcttcaaca gatggatgct attgtttatt cctatgctgt ggatacacct ctattgaaat
    30661 cccgtgaaag ctcctatggt gaatttatct ttaaagtaga tagttataaa atgcccgaat
    30721 taacacaaaa acttgcttct ctgggcaata ttgaacagca aatagaagtg gctgatacat
    30781 cactggtgaa taaatcctta gctactgaag aagaaatcct caaatctaaa agaaaagaac
    30841 tggcagacct ggaagcagca ggaaatatat atggctcact tgctgactat aaaaatagat
    30901 tgattactga aattcgtgtt caggaagata gagttaaagc acttcgtcaa tctgatatta
    30961 ctttacttta tgtacaactt attccatctg tggttagcaa tcaattttct tgggctaaaa
    31021 cctttgctct caatttcgtt aaagctctcg tgattttagt agtaggtgta gtagtgctct
    31081 attatatgtt aagattgata gcttatatcc tttcgctgtt cggagttaaa ggatttagtg
    31141 ccagtacctt aggtagtagt tatcgctatg gtgattacta taaccgatat tattcccgtt
    31201 atggttacgg gagacacaga cgtaaggtta aacgtattta caaagatggc agaacgaacg
    31261 atcaatctga aaataaggaa gaaactaagg atgaacctaa aacctgaagg aggtagctaa
    31321 atgcaaccgt ggcaaatctg gattattctg ggaataatct ttactataat tgaaatattt
    31381 gacccagctt tctttttcat tgctttaggt attgctgctc tcattactgg tttattgagt
    31441 cttttctata ttgtaggtag taatgttctg ctgcaaatta ttatattctc tgctcttagc
    31501 tttatctgct ttcttcttac acgcaaaatt gggaaaaaaa tactccagtt tcctacagca
    31561 gaaaccaata tttatgctct taaggggaaa acaggttttg ttacaaaagc cattcctcag
    31621 gatggaaaag gttatgtgaa agtaggggga gaagaatggt cagctatctc tgaagatggt
    31681 tctcctattg aggaaaatac taaggttatt gttctgggcg tggaaggtaa taaactgatt
    31741 gttaaaaaag ctcaataaag aattttctaa ttaatcatct aaataattaa atcctaatag
    31801 attagattgt gaagatagca ttaaataaaa atgatacctg gtatatctat aagtatttat
    31861 ataaatagta cccttttaat aattgtagca gattcaaaat aacaaaagaa tatattataa
    31921 attgtttcaa gaatgaaagg atatgacatt cttctctcgt agatgctttt ctgaatgctc
    31981 ttaataattc acttattatt taaggatttt ccgaaacaac ggagagtttt aatatttttt
    32041 cttccccata attaattggg aaacaatgag atgccagata tttaaaaata atttctaaca
    32101 aataacaaat cacataatta tcaacaatct ttataatttt tacctttcat cttggtcttg
    32161 attttatcct atctctccct tagctctttg ttgcctaagt agctaagaga gagctaatta
    32221 ggagccaagg gacagataag tgctctaatt aattgtctat gttggtgtta tcgttttttc
    32281 aggagaattt atcgcagttt aataaaatca aattgattaa ataagacata aatatactct
    32341 aaacgatata ttataaacag aatgacttta atttacgaga ataattgatg aaaaaatgga
    32401 aaattaaaat tctgtgttta atctagtgaa gaaattgagt cctggaccag ggtatccata
    32461 ctctgtctcg taataggtat caaaaatatt ttctaaacct acggcgagac tgaacttttt
    32521 gaatttatag gacaagccta tattgtgcaa ccagtaagaa gaaaggatat gataattatt
    32581 taatttatcc tgactaaaac ggttatcatt atagctacag gtatatctaa gttttaattt
    32641 ccagggcaaa gataaattct gagtaaagtt agcttgatga tgtggagttt cagttagttc
    32701 ataattctca gttccttccc accagatata tgaataggca acctcacttt cccaccaggc
    32761 aaatggtttg aacatcagac tgtattctgt gccagcagaa tgcactttat aaatattttg
    32821 gtatgtatct ccctgtctat caatcagatt tcgtgtatta ttataaaata agcttaactg
    32881 attgctaaaa attatattcc ctgctttaat tttctgttga tgactgattt cacatttaat
    32941 agcgttttga ggtttaaggt ttgggttacc attttccgcg gaaaatagct gacgcattgt
    33001 aggaaaagag atgttattcc caatggaaat agtactttta ctcagctcat tccatttaaa
    33061 attgagagct gcagaaggtt caggaaaaaa actgagtgaa ctcttcacat cacaatgatg
    33121 actgcttgcc cccaaaccag cgaggaaatt aactttatta ttcagctggt atttccactg
    33181 gcagaaggcg ttataaatat ttaaccagtg aggagtccac tgaagatagt ttccattgtc
    33241 ttttctggtg ctatgcatag attccaatct aaagccaaaa ttaaaagtat tccaagcatc
    33301 gggaacccat tccattcttg gattaaaacc cagagtatag taacgcatat ggctatccag
    33361 aataagatac tggtgagaag catcactgta ttccctataa atgtcctgac ctccatcgta
    33421 ataaagatga gaacttagct ttaagaattc attgagaata tctacatatt ccaaagtcgt
    33481 ccagtagtgc atccaatctg tatattgacg gtaatccaga gtatagatgg atgaaggaat
    33541 aagcttttcc ggcacataag tgaagccagc agaagctcct acctgtctga aaggattgaa
    33601 ctcatagtcc aaacgagtct gaaaatcata ttgagtcttc aaactgtgat ttcgcacccc
    33661 tccattttca aagggggtag gagtgaaatc ttttggtaat acgatgcctt cattatgttc
    33721 tcgtgccaga ctaactctat agccaaattt tttaagcttg tgatctgagc tgatttcaaa
    33781 tctattagca ttgtttcttt tagctaatag attgagtttt aaccagctat cttttcccgg
    33841 gtcagaagtg ataatattga ctactccgcc catagtgttc gttccataaa tggctgcact
    33901 gggaccttta ataatatgga tttcttttat gtcggaagga gaaaattgat gtaagtcaat
    33961 attgccgaaa taaccactat taagtggacg accatctacc agaactttca cttcattact
    34021 tcggaaaccc cgaatgcgta gattgctctc atcttttgtt ccagttgtat ttgtcactcc
    34081 tgcaatatcc tgcaatcctt cataaagatt gagagcagca atcccctttt gttctatata
    34141 atcaataata tgaagagctc cgatggcatc ctctggttta tctgctataa ctctcacggt
    34201 cgggagtaga tatgtcttct taaccagaga atccaccgga gctggattgg ttgttatctc
    34261 tgcaaaacaa ctcagagata atagaagtat taataaaagg gaaagatatc tggacaagtt
    34321 tacttttccg tgaccatttc cgtgctttca aaaccgctta aatcactggg tatggtaaaa
    34381 gtcaaatctg ctttttcttg aggtgccagg gcaaaatcaa aattctgggc agaagtagca
    34441 ccataaacat tgatttttgc cagttttttg aattttttca tattacccgg aagagtggaa
    34501 ttgaaattgg ggaaagttgt aacctctgaa tttaataaat aataaccatc gttaattagc
    34561 tcaggttgat agttcttact gtatccatcc acggctacaa aagctacaga atcataagca
    34621 gcaataccct gaagcaagtc actaagctta atagcatctt cggcagtgcc tgaccagtta
    34681 tccaccgtat aagtgctcag tccgcgcaat tcaatcagtt tagaacctct attaccagca
    34741 tctacatcca ccttacgata gagacgaaac aatcctgcat ttctaacttt aaaagcagaa
    34801 ggtagtccca ttccaggaaa ccaggttcta tggtcgtcat tgggaacata gtatccttgc
    34861 gaaaaagttt cccaggacaa gtcataacct gcataagcac tttgacgagg actgaatcca
    34921 tcagttgaac tgacgatttc ataagcaaat aaagaacggt attccaaatc tgcttctgcc
    34981 tgagggtcaa tatgaatacg cacggtatcg ggattgataa actggtcaag tttgtaacca
    35041 agaatcttga cctctgaggg attgtctttt tcactgcaag cagtaaatgc taagagcagt
    35101 aaaagtacta ataaataaat cagcttcttc atttatcctc ctaaaagctg taaaagaggg
    35161 gagatttaat tgctctcccg cctttccaaa atgggaggca taaccgtttc cagttatacc
    35221 ctgtcggtgt ttaatcctgc tttttgagtt tagattcaaa cactcggcgt gcttcttttt
    35281 tatgatacca gtccttattg tcaagaaaaa ttaaggactg aaataattat taagtcaata
    35341 agatttaaaa aagaaagtac ttaaatgacc aagactcacg gtatttttat tgacaagaaa
    35401 agctatctta ggtgttagga tagagcagtt aaataagtta aaccttctga gcatataaca
    35461 ttatttttga tagataccaa agctgtgaga ataagttcta aggaatttta aagttgaatt
    35521 ctatcagatt atacaaaata ggatttttag cggatttact tttattagtt ctttcaaagt
    35581 ttataaataa atgaggttat attgagatgt tagccgtaaa gaacctttcc cgtagttttg
    35641 gggatattaa ggcagtacag aatataagtt ttactgcaga taaaggggaa attgtaggtt
    35701 ttctcggtcc taatggagca gggaaaacaa ctactctccg gatgatggtg ggatatctgc
    35761 aacctgatag tgggaccatt gagttggatg gagaaaatgt gtttgataac cctcttgcag
    35821 ccagtgctcg tataggttat cttcctgaac ttaatcctct ttataatgaa atgaccgtgg
    35881 aagaatttct aagatttatc gctgctctgc gtaaaatgaa agaaccatat ctttccgaga
    35941 gattggagtt tgttagagaa aattgtggtt tggatgaagt atggaaacag aaaataggca
    36001 ctctttctaa gggttatcgt caacgcacag gtctcgctca agctataatt cataatccgg
    36061 acttacttat tttggatgag ccaaccagtg gacttgaccc caatcaaatt ctggaaattc
    36121 gggatttgat tcgtgaactg ggagaaaata aaactgttat cctttccagc catattatgc
    36181 aagaagtaca ggctctttgt tcccgggtaa ttattatcca taaaggacaa atcattgttg
    36241 atgatagcat agagaatctc agttcttata tttcagagac cactcagata gttcttgaac
    36301 tggaagcgga aaatccagat tttcaaccct ggctggaaca atatcctcaa gttcagctaa
    36361 ctgacataga acctaaagat gaccatttag cgttaaggtt cactataccc atagagatgg
    36421 atcttcgtaa ggaattatct gcttttgtat cagagcaggg ctggtatctt atcagtatgt
    36481 atagagaaac tcaaagtctg gaggaaattt tccatttact cacctcagag gaacctttct
    36541 atactgaaga agaagaaaaa gaggaagaag aggaagttat agaaggggaa gaaatttctt
    36601 ctgaagaagt taatgagtct gaggcagaac cagaagaaga gacaatatct ttaaaggaaa
    36661 ctgaagaaaa ggctcaagaa gaatcttcaa ccaaggagca aaaatgaacc ctgcattaac
    36721 tatagctaat aaagaatact ctctggcaaa tcgttcctta agcacttata tcatttatgg
    36781 tgtttatctt ctggcttgtg gcatctggtt tgctcttaat gcacttaaaa tcggagcggc
    36841 agatatgcga ggtatctttg gtgttatgca taccctgttt ttattcttta ttcctgcttt
    36901 aactatggga agcattgcta aagaacgaag cactggcact ctggagctta tcagtacttt
    36961 acccatcaag ctgggtcaca tcgtttgggg caagttttta ggtgttttat tccagttagt
    37021 caccgtcttg ctttttactc ttgtttttgt attattaatt agtctttttg gtataggggt
    37081 agattatgga gctatcttta ccggttatct tggtttgtta tttgctggag cggcttttat
    37141 agccatagga atgtttgcca gcagtctaac agataatcaa gttctggctt tcattatagc
    37201 atttgccatc tcaggggctc tgtttatcat tggtcgcata ggaaatttaa tccctctgaa
    37261 actttttgcc actattgaat actttggttt tgattatcat ctggaaagct tccttaaagg
    37321 ggtgattgat agtagagacc taatctggtt tgctgccgtg accataatct ttatcctttt
    37381 agctcaattc cgtcttcagg ctcaaaacct tagtcaggag cgataaaatg aacacacgca
    37441 atcaaatcat aacttcctat atcatcaaaa tcctgattgt agtgctggta gtaattattt
    37501 ccagtttcct ttatttccga attgacctga gtaaaagtaa gtcctacact ctgggcaaag
    37561 caactaaaga gactcttcat tctttaaaag ataaagttat agttaaggtt tttgcatcac
    37621 aggatttacc acaggaattg agcactataa accgtagctt aaaagatatg ctggaagaat
    37681 ttgcccgtaa ttcccataac aagtttagtt ttgaatatgt tagagccagc aataataaag
    37741 aactgattga acaggcaaga gagtatgata ttccagccta ttcttttact gcctatgagg
    37801 atgaaaaact tgttactaaa caggtggtct ttggcttgta tctggaaagt gctggaaaat
    37861 cttcctctat gtatctaaga cctggtatgg aacaaatgat ggaatatcag ctcttgaaac
    37921 aaattaataa actgcatcca gataatttac ctgaactaac cgtctttatg gattctctta
    37981 gtatggttca tcaatataca aattattcag atgccttagc tacttttttc cttgaattga
    38041 tggataacta tagaattacc tacactgacc ttaacacccg tcctaagtat acccctgtta
    38101 tgctatgttt aggaactatt gatactttga gcactaaaca actctactat cttgatcaat
    38161 atctgatgca aaacggaaat atagttatga ctcaggatcg tgctctgatg tttttatctg
    38221 agcgtggttc agctctggct cttctggatg gacctatatt taggcttctg gaacattatg
    38281 gaattcgtat agaacccaat attgtgctgg atatggaatg tgaatatggt agaggaagtg
    38341 gtttaggaac tatagtcccc tatcctttta ttccaagatt acgtcctaat cctaaatttc
    38401 cctatactgc tggttttcca gttatcacta tgaatattgc ttctgaaatt aatcccgttc
    38461 caaatgctaa aattaagttt gaaaaagtgc tccaaacctc taatcattcc aatgttttaa
    38521 taggaccaga ttttagagtg gatatagctg tccaaaaagg tttggatcct ggttatctca
    38581 gtcaaccccc caaaacagtt gctgctgagg tgtcaggcac ttttcatagt tttttccatg
    38641 cagcactgga tgacagtacc ttctataatt ccacggataa atcaaagatt atcctatttg
    38701 cggatagcga atttcccctg gaatttagtg caggagcatt tattgttctt aatgccgtgg
    38761 attatctttt aggtaggaac gatatgataa aaattcgttc acccagagat gtctataatg
    38821 aactcagcgc agaagtttat atgcaaaaac accagatgca acctgctgaa caagaaaaga
    38881 ctatttctat tttaaataga tattttcggc tgaccgctat tttcttgcct gttcttcttt
    38941 tggcactctt tggcattatt caagcttcaa gacacgcagc gaagattaaa tatgaagaaa
    39001 aataagatta tccttttaat cgtctttctt gctttgctgg cagtggctat ctatcttcta
    39061 agttctcgtc agaaaaaaga aaccctaaag cctatatttc cagctgattc tacatctatt
    39121 gccaagatag agattgccaa tcccgatacc agtatcactt tttctaagat aaataatcac
    39181 tggacgataa tctatcctat taaatgggat gtggatgaag accgtttcaa gatgttctgg
    39241 aacgaagtgc taaaagagaa atattccact gaacctgttg cttctggcaa agcagcgctt
    39301 gatcaatatg aattaaaaga ttccacagca cttaaaatca aggtctatga tagtaagaat
    39361 aaacttctta aagaggtgtg gttctctgat ttaggtaatc ctgcagatta tttctgtttt
    39421 aaaggggata ataatgtcta ccagataaaa cgaaaagtct gcttaaatta tcaaccaaat
    39481 ttagattcct ggcgttcacc ttatgcactt agaatattcc ctgaacaatt atatgctatt
    39541 gaagttaagc ataccaaaaa cagctatcaa ttgactcgta acggaaatat ctggcactat
    39601 aaagatagta aggaggattt tgatatcccg cctggaaatg tcactatggg taagatatta
    39661 aatgctcttt caagattagg ttcgggcaca attatgtctc ttgataaagc tccagaattg
    39721 gggagttctg cttgttctgt taaactttta ttaactgata attccactat ccagattgaa
    39781 ttttatcctg gagaaaagaa ttactttatg actattgacc tctatccaga cctggtcttt
    39841 gaagtgccat ttgacaccta tttccgtttt acaagatttg ctgctacttt ccgtgcccaa
    39901 gaaggtgatc cggaggaact tttacgctaa atatcatcca ttgcctccaa ttgaatgaga
    39961 atctctatgc tgaaatcata tctatctggg ataaaacagg catcagtaat cctgaacgtg
    40021 ccgatagttt tgattctatc cagagaactt tagaacatgg aggcattctt cttcttgcat
    40081 atatggatgc tatcttagtg ggaactatat ggttaactaa tgattaccgc agattatacc
    40141 ttcaccatat ggcagtttta ccagagttcc agaagcaagg gataggtcgc tctttattac
    40201 aagaagcaat tcaaatagct gataaacaag gtcttcaagc taagttagaa gttcactctg
    40261 ataatgctgc tgccctgaat ctttatcgtt ctatgggttt caaagtactg gatggctatt
    40321 tagttatgat tcggcgaaac tgaatcttag agtagaccgt atttatagga gaccccagcg
    40381 acgacggaaa aataactcca aacagaaagc tacaataaat aaagttaaca cataccattt
    40441 ccgataaaga gggatatctt tgcttttatt gaccaccctg ttttgagctg gaaggggttg
    40501 aaaactttgg agggtagcag gatatagaag ttttccttta gtttctgaag ttatccagga
    40561 aagtagaggt atattcaggt caaaatccat tgattccaga gaagattctg ccacattaaa
    40621 ctttcctgaa acactttgtt tactcatctg atctgaaata gtaaaactat aaataccagg
    40681 tttatccaga ctgaaatgag tggtatattc accttctgct tgaatcataa aatcacgata
    40741 tacttctttc ccacttgcat caagaatttt aagctcagga ttcaaatcca gaagcaaacc
    40801 tctaatatta tcttctgctc gcagtcttat attaatttct tctcccagaa aatagctgtt
    40861 gttataaata gcataataac ctgtggaaga agtgttagct aaccaagtta aggtattaga
    40921 aagtaatttt ttatagccac cactttcgct ttgcatttgc cacttccaga gattcaaaaa
    40981 tgccattgca attacttttg ctctaccttg agtgtttact gctatggcag gagagttttg
    41041 tggattgtct atagttgcca gaacctcagc tcctttagtt gatgtcacat aatagtaatc
    41101 caatggagga atattttgca attctgaact gacaaattcc agcataggat atctggctgc
    41161 ttgaggagtc aattccagaa aaccctgata aacactgctt atattggatt tttgtagggg
    41221 aaggatagaa gcaagttctg gaaggggaag tccctgaaac aaaattccta tccctttttg
    41281 ataggaagaa agaacataat ttagtggata tcccgaaagt tgtaaagcac cattattaag
    41341 aatgatgatg gcagatagat ggtctgaggg tatggtatca accagctttt ctccagcaaa
    41401 tactttatca ccctgaaccc gataatattt cacttcccag cgaggatttt ccgttacagc
    41461 atcaactata aatttattat cccaggaagg gctttcagaa agaataacca ctctttgttt
    41521 attggttagc acttctatcg ctccaggata actgttatta ttcaaagacc tctctttaat
    41581 tccaggaacg gaaacctcca ctctaaaagg ataaaatcct gtctggctaa agcggtaatc
    41641 aaaatccaga cttatagttt cacctgcttt taatgagact gtttgattac gaacttctgt
    41701 gttgccaaga aataaactga tcttggctga acctgaatag ttttccgaat ggatttctgc
    41761 ccttatgatg gtgggctcat tccgataagc ttgtggattg tagataactt ttaccacctt
    41821 gatatctgct tgtaaagagg ttgcggtatc acccagagca taaaagggac agcctaattg
    41881 ttttacaggt ataggaaatg catcatgtaa ccagccatca gaaagcaaaa ctataccctt
    41941 tatcctactg aaatcatgct tcttatttag ctcttccaga gcaggaacaa gaagtgtatt
    42001 attcctgtta ttggtcaaac cagaagcaaa atcgtaggaa attatctcat agccagctga
    42061 gctaaattta tcttttatgg tagaactata actctgtatc cagtttttct ttgttttccc
    42121 attgccacct ggaaggtcca tactggcaga gatatcggaa agcaggataa tctgttgctt
    42181 ttcatttact tgctgaacat aatgaattat cggactgata agtagaagca agataatagt
    42241 aagacttatg cttctcagaa agaaaagcaa aaaacgctta cctttttcta aggctggttg
    42301 agttctataa tagtaatatc ctgatataat cagagatatc agaattatga ttatgtattg
    42361 cagtactata acctcacatt aaagtaagta gcaagggcaa gtcctttagt tgaagaatta
    42421 aagaaggcac tctctataga ttgtatcgca ataccgaaac ctactgattt taaatccaga
    42481 cctatgccat aagacatacg ccagggatag atttcattgc ctgaacaata acccaaacta
    42541 attggaattt gcggaagagg acaaatctgt gctcccagtg aaatacgacc ctgtttacta
    42601 acttcaggag aattgccaaa tcctaccaca taatctgcag aaactgagac tggcttaaat
    42661 gtatataaag cagataatcg catttcagga ggtagtttag ttgtataggg ttttatatcc
    42721 accgttgagc tgtcttcaat ataaaagtct tcatctatat ttgaagcata aagattgttt
    42781 atctcaaagt gataaatttc atcttgagtc tgagtttccc atcggataaa accgaataga
    42841 ttatccagag tagctccaac ttctaaacga ggtatgggtt cagaatacag accaagcata
    42901 gctttgagtc cataaccgcc tataccagtg cggattataa catcctgctg gacatttaag
    42961 ccatccaaat tggaggaaag atagccatct aatttacgag tttgaacatt gcctaatcct
    43021 ccaagtgcac tcaaggaaaa accgaatttt atatctggga tggtttcagg aagaggaagt
    43081 gatatgtcac caattccata agtcaggtct gcatagccca agccttcagc atagttatta
    43141 tttttggtaa aatgatagac ttcacttcca tctccatttc cataaagaac aaactcaaga
    43201 aattgctcat ccagagctcc tttagctgct aagtgaacgg aaccagtgaa tgacatatta
    43261 tccgtggtaa aaccaataat tcccatattt ccacctatac taaaggacat attaccatct
    43321 atagcattca aaatcttctg cttagcttca tcatccaggt aatcttgttc cataatatag
    43381 ttgtataaat ctatattaaa agaattattt cccagataaa tgctgttatt gaagaaggga
    43441 atcataattt cagaataacc tctttgaagc aaagcaggat tccaatagtt ggcatcacaa
    43501 cctcttgctc gtaacatata actatcggca aagaacattg attttggtgt tactgttgta
    43561 gcagaaagta ttgatagcgt taaaaatata atcaatccaa taataatctt tttcattatt
    43621 cctcctcaat atgaattctt acagtaagca tacctttcat atgaatataa tccgcgggag
    43681 aagcataaat ggttacggga ccatcagtcg cttcaaagct gaaggaaggt aaaagataca
    43741 cttctggatt ctggaaaact tttatttctt cctgttctaa agttagattt attaattgct
    43801 ctccctcaga atttacatcc ggacctatat attcagagga acgaatagca aactgcttat
    43861 aaaactgata agattgagga ttagatggat caatatttgt atcagtaccc acataaatag
    43921 tagcagtagc cccaataggg agctgatttt taactttcat taccattgca gcataggtga
    43981 ccctatcacc aatctggtct cttacatctt taggcatttc taccttagat ggttcttcca
    44041 tagtaaaagg attatcagta agaataaaag tgaaaggtgc atctatttga taagtgcaac
    44101 gtaatctatt agtagaatta acaaagccag ctgtaccatt attgcctccc tggattaata
    44161 aataggcatt gataaggtta acttcatctg gcataatttg aagcacctca tttattctat
    44221 tagaaaattg aagttcagtg ataacagaac cggattgatt ggcaggagct atataaaaag
    44281 gcaatccatt ttcatctaaa ataggtatgg ttttttctat gccggtccta tgattaatcc
    44341 catatagttc tccataaaac tttacaccaa aaccaaattc attgttcatt aatagacaaa
    44401 gtgaagcttc ttgcaattgt attgcttcat caattccaaa aggataatca atgtttatag
    44461 tgacagagcc aggttccata tccagctggt aatcggaaag ataaccttca aaagcaacaa
    44521 aacttagagg ttggtctatt tttaaacctg ctgtgccaac tggtgttcca tttggcagat
    44581 ttgattgtat ggtaattacc acagataaag cattcaaaac ctgtccagaa tgctctaaac
    44641 ctatagtaca tcctgctaaa tcaacatcct gccagtacgg ataagagctc ccactgatag
    44701 ttaaaggatt acctgaagaa gtgcgtatat cagggaaact cagggaaacc tgcgtatttg
    44761 ccggatcatc taagtcataa taataagaaa gcgtcccatt atcaactaat ccataaatta
    44821 attctactgt atttgtaggg tcaataaaag ggatagtcac attgcccact attccagaga
    44881 gtaaaggtat agggtctaat attgcttcgg gattaaaact tacatttcca aattcctggc
    44941 tctcagtttc acctgtccct atcaaggtca gaacattatt ttcacctacg aaaagattaa
    45001 cactatccgc taaatcagaa acaaaatatc gctcatttat caaaggtaca gtaagttcca
    45061 catcccaggt aggaagatga ggtttgctta tctcacaagc ggaaagtaaa agtaaagcgg
    45121 ctattaacat tagagcaatt tttttcataa tttccatcct tcttcgctta attagcgata
    45181 gtcatattat tacattctcc ttaaagagta gaagtgtcaa gagatttctg taaaatggca
    45241 tttattattt gccttaataa atttatttgt ttcattcttg gtgaatccta aatatgtcta
    45301 attttccttt aattattaga caataaacat tttagatgat atatttgtcc cctgactcct
    45361 acttgactgt ctcttaacac cttagctaag aaagagccaa gagagagcca ggatagacca
    45421 gacagaaaag aagggtaaaa attttaaatt cattagaaaa aatatatcag caatgaaaga
    45481 agataataaa aaatattgtt ctcaaagcac ttctacttta acccaatatc attcctataa
    45541 ctgctgcacc taacaagaga tatataggat tggttttcca gcgccagcta acaaatagag
    45601 cagctacaaa tatgattata gaaaagaaat cggggaaggc tgtttttcct ataagtatca
    45661 ttgcagcagc aattaagccg agtgttacaa aacgtaattt ctggaatata ttttgaaacc
    45721 agggaagttt tgaaagtttg atataagtta aagtgatagc taacattaat ataatagaag
    45781 ggaaaataac tcctaaagtg caagccacag aacctaaaat gccaccctgt ctataaccga
    45841 tgaaagttgc tgcattgata gcaataggac ctggtgtcat ctgtgaaatt gcaactatat
    45901 ccgcaaattc agaactggtt atccaggcat tttttgttac tacttcctgt tgaatcatag
    45961 ccagtatggc ataaccacct ccaaagctga agagccctat tttacagaag gttaagaaca
    46021 ttgaaagaag cattattcag accgattctt ttgccagaga ctctgtagaa cactaaaaat
    46081 tatagtagtc agtataatcc agatagggct gatattcaaa aatgccacta aaatcataac
    46141 taagacagga acccataaaa aagcaggttt aatctgagaa gagcgcgtca tttgcaataa
    46201 aggtgatgct attagagcta ctacagcagg tttaagagca tgaaaagcct tttgtaccag
    46261 agttaattga gcatattgag tgaaaagtgc ggcaataata acaataaaaa caaaagaagg
    46321 aaggaccgta cctaaaacgg caacaatcat tccccagaat ccttttagat gcataccaat
    46381 atacacactg aaattaactg ctataggacc tggacaggat tgagaagcag ctaagccatc
    46441 cagaaaggtc ttatcatcaa gccaatgatg tttatcaacc acttctctgc gaattaaagg
    46501 aatcattgca tagcctccac caatggtgaa agcgccaatt tttagaaaag taagaaatag
    46561 agagaataag gagttagaca ctttctttcc agtttttaat caattgaatc agttcagcta
    46621 ctaatctatc ttctggaacc tttttaatta tttttccttt agcaaaaatc aatccttcac
    46681 caactcctcc agcaatacca aaatctgctt ctcttgcttc tcctggacca ttgacagcac
    46741 aacccataac agctatggtt aaaggcaaat ttgcatattc ctgaagtgca ctttcaactt
    46801 cttcggcaag acgcataaga ttgatatggg tgcgaccaca agtgggacaa gaaatgattt
    46861 gcaaaccttt acgcaaaccc agattctgca aaatttgttt ccctaccata acttctttta
    46921 ctggatctgc cgttaaggaa acccgtatag tatcgccaat accttcttct aaaagaattc
    46981 ccaaagccag agcagattta acgcttcccg aaagaagagt tcctgcttca gtgacaccta
    47041 agtgcaaagg ataatctatt ttttgactta aagcacggta actggcaagc ataagaggaa
    47101 cttgggaagc cttcacagat actttaattg cttcaaaacc tatatcttcc aaaatacgga
    47161 tgtgggataa agctgcttct accattgctt ctgagcttaa accatattta gccagaagct
    47221 ctttaggcaa agaaccacta tttactccaa tcctgatggg aacacctcgt tcttttgctg
    47281 attttgcaac agcttccact ttgtctcttg aacctatatt tccagggttt atccttagac
    47341 catccactcc tgcttgaagt gctgccagag caagtttgta gtcaaaatga atgtctgcta
    47401 ctaagggaat atgcgtttcc ttcttaaatt catagataga aggaatatct gccattgttt
    47461 caacagaaaa acggatgatt tcacagccaa ctttttccag agaatgaatt tgactcaaag
    47521 ctgctgatat atcggaagta ggtacactca acatactttg cacagatatg ggagcacctc
    47581 ctccaatgag gacatttccc agtttaatct ggcgggttgg tcttctttta atagtttcca
    47641 taaggctatg cacagtttat agttaattct gtcaatcaaa tttactttat acttgaccaa
    47701 taataaacaa catataattt ggataaatga ttaatattag atataaggta aatcatagga
    47761 ggtttcaatg cataagcaaa ttttaataat ggcattaatg ctgcttctgg cattttttgt
    47821 tcaggcagaa gattggctaa ctatctataa tgatgacctt tctctggtac gttctaattt
    47881 tgaactggaa ttaaagcagg gaagacagga ctataactat acagatataa cttctcgcat
    47941 tgaagctcct tcggtgatag tgctttctca ggatggagca gttagagtgg cagaacaaaa
    48001 ttatgaatac gatcttgccg ggaaagccca aatcttgaat aaatatatag ataagaaagt
    48061 ggatttattt acaaaagata acagtcatct gactggtatc cttaaattct atgatggtat
    48121 aagttttggc ttaattgaag aaggaacaaa tcgtctgatt gtagctgata atgatcaagt
    48181 tcagctcatt cagttagcag aacttcctcc caatttttac accagaccca ctttacactg
    48241 gaatttatta gccaataaag ctggtaaaca tcaactccaa ttaatgtatt taactggagg
    48301 attgagctgg gatgtaacct ataacacggt ctgggatggt catattttag ctttgaattc
    48361 ctgggtaacg attactaata atagtggcaa ggcattcaat aatgtccatt taaaactaat
    48421 tgctggggat gttaatagag tccgtaaagg ctattatgat acggcaggaa gaggttatga
    48481 agttatgtta aaaactgctg atcaagctgc tccttctttt gaagaaaaag ctttccacga
    48541 ttttcatatg tatactctgg atcaaccagt tacctttgct gacaagcaga ccaagcaact
    48601 tgaactttat cctcttcaaa atataactgc ttcccagttt tatgaatata acacttttgc
    48661 cagtagtgtg cttagcatca ttaaatttaa gaatacagaa gcaaatggaa ttgggaaacc
    48721 tttgcctaag ggtactataa aggtctataa aaaggatgta gataataatt tagaatttat
    48781 tggtgaagat agtatcaatc ataccagtaa aaatgaagaa gtgagaatca atactggttc
    48841 agcctttgat ctggtaggta aaaccatagt taaagatagg aagactataa gtcagtatat
    48901 tagtcaacgc actattcaag ttactcttag gaataattct aatgaaaata aatcaattga
    48961 tgtgattcat aatttaggga ctaacgctaa aataatatca gtagaaaatg ttactcaacc
    49021 cactgtagat gaatataata atgcaacttt taagataaat atagctccca atcaggaaat
    49081 tattttctcc tttgtggagc gaacagaata ttgattaatc tcaaagtaac agaggatata
    49141 acaatgcaag aacgctgtta tgataactta aggagtttga gaagagagat gctaaaattt
    49201 ctcagtgatg catctttgcg ttctcgggac cctttaacta tggaaaatgc tattgatgac
    49261 atctggcacc ctaaatgtga tgtgtttcaa accgattctc aatggataat tttagtggaa
    49321 cttgcgggaa tggagaaaga agatataagt atctcaagag gcgatgatta cattaaaatt
    49381 agcggagaaa gagaccttca tccagaattt gaacatgcta cctattacag tatggaaatt
    49441 gaaacaggta gatttgaaag aattatctat ttcccggatt taaatctgga taaagataat
    49501 ccccaggtgc aatataagga tggtattttg cagatagcat ttaatattga agcaaccata
    49561 gaaagaataa tccccataga ataaagttag ttactgacac ttattctgtt aagaaaagtt
    49621 agcagtagat gatatcagac tgaaagaggc atagagtcaa agggaatagc tggtgctaaa
    49681 gaggttagga ttttgttttt tgattgctgg tatttgtttc ctcagcgctg aaaccagaat
    49741 cctttctatt cgttctgaag acctatcttc cacaagtaat aaaatatata taacggtttc
    49801 tgattccatt cgttatagcg tgcaaaaact aactgaagcg aagggagtta gagtcatatt
    49861 atacgatgtg gaaggaaatg tgaaaccttc ttatcccaga ctaagtgctg ttctggatag
    49921 aattacttgt tatccagctg ggaataattt agtagtagat attattacta tgggagatta
    49981 tcctgtaaat tcatattttg aatcagggga aaatctactc actttaacta tacactcttc
    50041 aggagcagga aaggcaatgt cttccaaggt attaggaaaa gcagaaaaga ctcttaaatc
    50101 agaggctgtt tctcaaaccg taaacaagga gaaaattaag acttcttctg gaaatactaa
    50161 taatgagttt gggctttctt caaataaaat tgaagatata ccatctcagg aatcagtggc
    50221 agatagttta cagctttctt cttcagatac gattagtata cccgttgaag aagtgggaaa
    50281 tgagcccaac tgcaatttac ccagtttctg gaacagatat aaatacctga ttttatcaat
    50341 tattacggca gtttgtctct ttttcgttct taaatcttta tttaaaaaga aggcgaaaag
    50401 accggggaat gaggataagt ctggtgaaga aagtgcactt cttttgcctc cagaaacggc
    50461 taaacttatg gtgcagaaac tgctggataa ggactggaag cctgaagaga ttgcacgaga
    50521 attacatctt agtgtaaaag aagttaataa aataatctca gcattaaaga aggaatcgga
    50581 tgaataggat accagcaggt aagttcatct ttgtgtttgt tatattttta gctggaatat
    50641 cattctgttg tgcctattca ttctgggaag attcagattt aatacctgaa gtaaggacgg
    50701 aggtctacaa cagtatctta tcaattaatg acacctatca ttccgatcct catacgataa
    50761 atagactggg agttaatcat ccttttcaga cccttcgtaa gttccgtaga tgcgcaagag
    50821 aagcttttct tcccggtgaa aagtcccatt cttttgttca atatgcctat gcagattctg
    50881 agctttacta ttctatggat ataaatttta ctgcaggtta tgaccagcgt tcggatgacc
    50941 ctggagatta tggttttttc tataagggtt taagattcaa ttctcaatgc aatgaccgat
    51001 tcagagtgcg aggaaactgg tggaatggtg cttttcacgg ttccagaatg tactgggaac
    51061 actctcccct aattgatggt tataataacc agaatccgga taaaaatctg ctggataata
    51121 tgaatggcga tatcagctat aataataaat atttcaatgc tgcggtgggt agaggaagat
    51181 ttcagatagg taactccgtc tctggttcta tgatactctc agataaggtt aatgaatatg
    51241 attatctgct tctggaacaa agagtaggaa aatttagctt ttccttacta aattcaatac
    51301 tgaaagctga tagtaccaaa actccagatt cagatgatta tccagataag ttttttgccc
    51361 ttcatcagat gatgtataag cctctggata agctggaatt atttgttggg gaaacggtgg
    51421 tttatggcag tcactatgat ctcagttata tgcttccttt ctacttctgg agagttggaa
    51481 agtatggaat gcaagagaaa gataatctta tgatttatgg tggtgcaaac tactatccag
    51541 ctcctaatct ggtcgtctat ttaaatgtgg caatagatga acttacttat aataaaatct
    51601 ttaccgattg gtggggaaat aaatatggaa ttcaaacagg tgtctctatg aagataccag
    51661 agtttgcact tacggataat gaaagtccaa gactgacagt ggaagctact gctatccgac
    51721 cctggactta cactcattat gataatgtaa caatgtattc caatgagcga ttgcctctgg
    51781 gctatcctaa aggttcaaat ttggtagatt taaccgctga attaaatctt cctttgccct
    51841 ggaaaatgag atgggatagc cagttttctt gcacctggca aggtagtgtg ggcaatgatt
    51901 ggcgtttaaa ttatcatacc tactttccta gcgatatagt taataccgca gaagcttact
    51961 ggttagaagg tgatgtaagc ttcattccta cctggactaa tgctattcat ctggatttta
    52021 tggcacatca tagttttatt ctgggtcata gttccacttt tagcaaagat tctcgtcatc
    52081 aattgtttct tagttggcag ttcttttatt aattatgcct attgtagacc gtctggaata
    52141 tcctaaaaaa tataagatga gtagtgctat tctactctta gcagttcttc ttgctgttgg
    52201 tgctctactt taccaaaagt atagtaataa agcaatggca aagcagatag ttattcatag
    52261 tataggcttt gatgagtatg acaaaaatta tattagagtg ggatatgaaa tagaaaatat
    52321 gggtaaaaaa gaccaggaag tggcactctt aataagagtc tatgatagag aaggtggaga
    52381 gatagccagt atatttttta agactaacat taaagcccat acgagagaat ttcagagtaa
    52441 ggttatagat gaattgaacc gtccgttgaa agaaggagag aaaccctata gagcaaccat
    52501 agaacttcgt cagcgggaat ttatgcacta ttaagctctt aagttatttt tgcttgacat
    52561 aaacctaaga tattattttg aagataaata aatgaatttt taagattctt atccgacgat
    52621 aaaaaaataa aggagaaaaa agatgacaaa agcagatctg gtaaaaatca tatccgaaag
    52681 taccggtatt atccgcaaag atgttgcagt agtcgtagat gcttttttgc aggcaataaa
    52741 agatagcttg agccagggga atcatattga aataagggga tttggaactt ttaaattgaa
    52801 aacccgtaaa cctcgcgtag gtcgtaaccc caaaaccgca caaagagtgc ctgtaccagc
    52861 acgtactgtc cctactttca agttttcccg tgagttcaaa aattccgtta gcaatgtaaa
    52921 aattgatgag tagagtgttt atatatgcct tgcggtagga agagaaaacg tcataaaatt
    52981 gcaactcaca agcggaaaaa acgtctacgt aaaaatcgtc ataagaaaaa ataaaagtga
    53041 ccaccctgcg gggtggtttt ttaatcttaa aaaggcataa cttatatgga taacgaattc
    53101 tgggaagtcg tagccgaaga attaaaaagg ctcctacaag gagatttttc ttcgcataag
    53161 gatttcccct atttctacac taaatattct tatccaatag cttctcctct tgatggattg
    53221 cctattcttc cccaaactat ccctgaagtg ttgctcaaat gggcacaaat ggatagtgaa
    53281 agtcaatgga atccagagga ctttttgttt ctggatttag agactacagg tttaggacgg
    53341 aaccagactt ttgcctttct aattggattg ggatattatg agaaagacca gctggtggtg
    53401 gaacagattt ttcttcctgt acctgaagct gaagtcaatt cctttgaccg tttagctgaa
    53461 ttgctttctc gtaaagctgt tctcatcact tttaatggta aaacctttga tattccaatt
    53521 ctggaatcac gtttacttta taatcgtatc tggcttaatt tacgcgaaaa agagcatatt
    53581 gatttgcttc aattagccag aagattgtgg aaaaataagt taccttcttg tgctctggaa
    53641 accttagaat tctatcttat gggaaaaatc cgagaagagg aatgggatat agatagtgaa
    53701 ctgatcccgc aaacctattt ccaatatctt caaactggag aaccagaagc actgaaaaaa
    53761 gtatttatcc acaaccaact tgatatactg catactgcag cactttttac tcttatctgt
    53821 gattctattg attatccacc agtggcaggt tacgaccaga gaattgatta ttatgctctc
    53881 gctcagctat atctaagaca gggcgataaa gataaagcaa aatatatttt atcagacctg
    53941 ctaaaccaga attatttgac tggtgatgtt gcttgtgctc tgggaatctt atgtaaacaa
    54001 gagggaaata taataggagc ggaaaggtat tttgcaatag ctacagattt atttcaccct
    54061 gatggaatgc tggagtatgc taaattactg gaaagaagaa aggcttatgc tcaagctctg
    54121 gaagttgctt tgaaattatt acactggaat ctgagtcgtc ctctggtcaa tagtaagaga
    54181 attctggaat tagaaaaaag agttaaacgc ctccagcgga aaataggaga ttaaaacttt
    54241 tccattactt agctgaagcg tattttatat tctttctatt ttggtaattc ctatgattta
    54301 ttattcaaag tccgctcatt taatattgct aaaatagcat atataatatt tgttttattt
    54361 attaaataat gtaaacatat ttgtagattg gaaacgccat taatattatt cttaatcacc
    54421 tgatacagaa tacattaacc tcacatttag tccctaaagt cctacttgac tgtctcttag
    54481 caacttacaa gcaaaagagc taagggagag ccaagagaaa attcagagga aaataggaag
    54541 ctaaaaaggc acaatgctct atgacaataa tgatagctaa tcctatatgg cagaataaat
    54601 aaatgggata atctatagaa actgaacaaa aaacatcaaa ggagtaaatt agattttagg
    54661 ttaattaggg gaatataggt tattataact tataactttg tctttatgtt aagtaagagc
    54721 aaaattatat atgaagttat tacagtagga gctacgattg ctaatagcgg ttcaatgggc
    54781 agataaaggg caaaatccga agattaataa tagattataa attcattccc agggtcagaa
    54841 tttatctatt gattatccac ttttgcactc gctcttttat tctttctttt tgttcttcag
    54901 taagctcgtt ttccagtaga gccctgtttt cagcagcagt tttattgcca ttaacttcgg
    54961 ctaaagagaa ccagaagtag gcttcttcca ggtcttttgc gacaccttcg ccattcgcat
    55021 aacaaactcc taaattacat tgggcttgag gatgaccctg ttgagctgcc agacggtacc
    55081 atttgactgc ttcatcatag tctttaggaa ctccgatgcc aaatttgtag caaactccaa
    55141 gattatactg tgctctcggt tcaccctgtt ccgcagctaa catataccag cggattgctt
    55201 ctttgtcatc tttttctacg ccgcgaccat ttttataaca gattcctaaa ctgtattgag
    55261 cacgagcatc tcctttttct gcagccatca accaccaacg aactgcttct ttataatcct
    55321 gtgggactcc ctctccttga tagtatttca ttccccattc atacatttct tctgccgttg
    55381 taggatatat tggaacttta ggtgctggag ttgctggttt ttgtggctct atggcttccg
    55441 gttttttagg agaaatcgga gcaggagata ctgctttttc tatagactca tcagaagttt
    55501 ttgctttctc agattttaat tctgtggtaa gagttgaagg agttgtaact gattcaagac
    55561 ttggatagta aatcccactt tttttaagtt gatttattac tataagattc cccttagttt
    55621 tcttttcctg ttcctgagct atgcgatacc atttttctgc ttcttgttga tttttaggga
    55681 cacccaatcc gttttcatag cataccgcaa gattatacat agcgtcacta ttaccttgtt
    55741 ctgcagcatt aagccaccag gacactgcca ggtaataatt ttgctcaaca ccttctccag
    55801 caaaatattt aaaacccaga ttattttgag cataagaatt gcctttatta gctgcttttt
    55861 gataccactg agctgcttca gttttatttt ctgccactcc ataaccatta tccagacacc
    55921 agccatataa atattgagca tctggattac cttgacgagc agctattcgc caccagcgcg
    55981 cagcttcaac ggcatttttc tctatacctt cacctttaaa atagcaccaa cccaaatcac
    56041 attgggcagc agaatttcca ttatctgcag ccagagccaa ccattttacc gcttcataca
    56101 aattgcgagg tactaacttg ccttcatagt agattctacc cagctcatat tgagcatcgg
    56161 tatctccatt tttagcagca aagagtaatt catcaatata actactctgg gcgtaaatag
    56221 ttagacataa taggagagcg ataaccatta atataaattt ttgcatctgg atacctccta
    56281 agctcaagaa tgtttgttat tataaataaa acattctgtc aagaacgaat actattttcg
    56341 tgagctttta cttcacgata gagaacctta atactatagg tttagttcta ttatcgggag
    56401 aaatacgcat cagatagata ccagaagaat atttagcaaa tggaagatta atttc
//
LOCUS       DAORZF010000006        51039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_562_length_51039_cov_348.953260, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000006 DAORZF010000000
VERSION     DAORZF010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 51039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 51039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51039
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_562_length_51039_cov_348.953260"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="PK015_01675"
     CDS             <1..228
                     /locus_tag="PK015_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33217.1"
                     /translation="LLGAGIITSYLYSLYLLTSTPFTKSDGLSFFWTSIILFVFVLFG
                     ISLADMFIVRYQTRWIKAEQKETEESKNQDD"
     gene            221..829
                     /gene="trmB"
                     /locus_tag="PK015_01680"
     CDS             221..829
                     /gene="trmB"
                     /locus_tag="PK015_01680"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF014447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOL33218.1"
                     /translation="MIEDRDFFVLDNVQERIEVPALFGNDKQLYIEIGSGKGEFISQY
                     ALLHPEWNFLGFEAREKRIRNILKKLSPSTHPNVRLVRMLVNSNIASLLPPESVQGVF
                     IQYPDPWPKKRHHKRRLIQQDFLNALATIMVPDAEVHISTDDLDYANWIAEEFLKNPY
                     FISVQDEIIQKIPSLEDHIPSWYEAKQTHKGISPNYMLFKRI"
     gene            939..2258
                     /locus_tag="PK015_01685"
     CDS             939..2258
                     /locus_tag="PK015_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33219.1"
                     /translation="MNKNIELQQLSIFDNYSYPSYEEILEEYSKEFENYVLPKGDTIF
                     GFWMQTLADLEFLDLELQGLTEDYTIDPINRKVKFSGDEEFIRLRIAHLEKVKNKPTL
                     YTDFVDKFGDTNAYAFHNLYPYKGKFYPRIVRTLINTFNLNENSLLVDPFNGSGTTTH
                     ESSLMGIKSMGIDVTPIGIVLSQLKNDLLFTDENKLNFSVKELQEILEVIKNKKWQHN
                     DPLIYKLMLIVYFDTIDAFKRTSRYNRKGQIGLFIEKYNYIKECHTKIRDIKNEYNLI
                     FERAQIIDGDILELKNMYDLEGKFDACITSPPYYFSIDYVGKDKIAYDYLDVDMKKVE
                     SKYLGMKSNGNSKSIYSGFPIRVAKYYEDLKTSIENIYWMLKLGGRLAIIIGDSTVNG
                     KKIPTTMITKKFCEEIGFKFEKLIFNPLLGARNRAIRGESVIICIKE"
     gene            2261..3523
                     /locus_tag="PK015_01690"
     CDS             2261..3523
                     /locus_tag="PK015_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33220.1"
                     /translation="MITKRNLICVKNKEKLELGHLLLYEPYKNILENFKKLCIDIKEK
                     SFDPVAKVYDGLLSVPNETREYYEALLGVSSYYQHSQGGRGKYIEKKIASSFETCTLD
                     ISLSKIPYWLTYPSLHKKQGLFTQRALSREEKEKLRTIEWEWLGDRDVNTDVGSIISE
                     ENTIVLVEIKNRVDSGGTAARREIWTSEKFGLYFDYLINNEKIFQKNLNKYSLSELLE
                     SFGINNLEIYIGILFDTNDSTATIESDRIKGFYSSSKQGFEYLKNKISQNSSIYIINE
                     DLDNLFMELGLSYSKLKIKIGALYGDDITLKLFRQKFPVSDLLLLKYDDIWLSQLISI
                     DERTILLKYNMNYTKIFIDLLDRSNDLRKKYDILIKSECQESELFEIVNYLLTNYNDK
                     FNDKFLPPGKEKSYYLGDLIQFLCAVDA"
     gene            3720..4688
                     /gene="dusB"
                     /locus_tag="PK015_01695"
     CDS             3720..4688
                     /gene="dusB"
                     /locus_tag="PK015_01695"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOL33221.1"
                     /translation="MRQLVILTNHKLWLAPMAGYTDRAFRHLCKEYGADVMVSEMVSA
                     DGLIRNTKKTLPYILFDGLERPFGIQIFGSEPYIMAKAAELCLAWNPDFIDLNMGCPA
                     KKVVRKGAGAALMKNPVLAAEIVREVKAVLDKGCFLSAKFRSGWDLKSINYLDFGLRL
                     QDAGADFLCLHPRTQSQMFSGTANWTHIAELKNQLSIPLIGNGDIQTPEDALRMYEET
                     RCDSVMIGRGALGKPWIFHQIHQLEKNNDYTPASRQLILETMLRHIDLATHFKPESVA
                     VKELRSQLCFYTKGIIGGAELRQAINHAVSAEQIKELIKSCEGLKF"
     gene            complement(4915..5328)
                     /locus_tag="PK015_01700"
     CDS             complement(4915..5328)
                     /locus_tag="PK015_01700"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA];
                     GO_process: GO:0006183 - GTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006228 - UTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOL33222.1"
                     /translation="MIEQSLLIIKPNAVVHHHIGHIITMVEQAGFNLKYLQTFQFTPE
                     LASVFYEMHKGKNFYDRLVNFMCSAPCVGIIVEKENAIEDLRDLIGDVNPEKRKVGTI
                     RDFFAEGITENAVHASDSKESAEREINIIFPQFGT"
     gene            complement(5325..5648)
                     /locus_tag="PK015_01705"
     CDS             complement(5325..5648)
                     /locus_tag="PK015_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33223.1"
                     /translation="MPNMEDFQVNYDNLLKLGYAVIDVRYKEYDVCNDSFKLVIGRVD
                     SDRDGFYTEMVKMYTAIDFKPNEIYEIWTEILIHKIKMSQVLNRDIAIKVAALDYIET
                     VYKAR"
     gene            5842..7851
                     /gene="uvrB"
                     /locus_tag="PK015_01710"
     CDS             5842..7851
                     /gene="uvrB"
                     /locus_tag="PK015_01710"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOL33224.1"
                     /translation="MGIFKLHSDYLPRGDQPEAIAALTEGLRQNKRYQVLLGVTGSGK
                     TFTIANVIAQFDRPVLVLSHNKTLAAQLYGEFKQLFPENAVEYFISYYDYYQPEAYIP
                     GKDIYIEKDADINEQIDKLRLRATMSLMERRDVIIVASVSCIYGLGVPEDYREALIHL
                     KTGMQMERDELLEKLISAHYSRNDIAFERGTFRVRGDIVDIFPAYQEHFIRVEFFGDE
                     IVRIEKFHPITYQSLEEVPEYSVYPAIHFLTNPDRLKRAIASIESELDERVAYFLSQQ
                     KYLEAERLQTRTKFDLEMLAELGYCSGIENYSRHLTGAKPGEPPKCLLDYFPDDFIMV
                     IDESHVTIPQIQGMYAGDYTRKKNLVEYGFRLPSAFDNRPLRFDEFQKYMHNVIFVSA
                     TPADYELSLTNGEVVEQVIRPTGLLDPEIEIKPIKNQVDDLINQIRERVAKNQKVLVM
                     TLTKRMSEDLSQYLQKAGIKSKYLHSEIDSIERAQIIRSLRLGEFDVIVGVNLLREGL
                     DLPEVSLVAILDADKTGFLRSARSLIQISGRAARNVDGKVVLYADEITDAIKKTLEET
                     NRRRAKQLAYNQEHGITPQSISKTLQQIMESTAIAEGYDAMEKKAKANHFRKEDFYDY
                     LELDNKEKLLQILKKEMKQAAAKLDFERAAELRDTIAELEKQANS"
     gene            complement(7860..8750)
                     /locus_tag="PK015_01715"
     CDS             complement(7860..8750)
                     /locus_tag="PK015_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOL33225.1"
                     /translation="MLTIFLLFSCGTNHKLTPPENVNILEFGTESTLEVVTWNLKTFP
                     LNNNTVQTISQIIPQFKVDIIACQEVMDQNAFYQLASAIPQYEALVSNATSSYRLAYL
                     YNKETVQVNEVYPIFIGESNPFPRPPYVLDFNYKGLNFICINNHLKALGDDYIDESDA
                     WDEEVRRRLACQKLDQYITEHFPDRRVIVVGDMNDQIAEPPSTNVFQVFLDKPDEYYF
                     TDYPIALNPTYDNVSYPHYNPPSHLDHILITNELFDAFAQADSVCRVIKVEDYMGSWS
                     TYSSIISDHRPLGVRLYFSR"
     gene            8932..12438
                     /locus_tag="PK015_01720"
     CDS             8932..12438
                     /locus_tag="PK015_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOL33226.1"
                     /translation="MKRYLWIAIMLAVCCLAAAMNENYITTTTGLLAHLENVRVAPVL
                     QQPEEPEEFPETTLISKTFALPYNSIDLQVQNLQWNVFDSSGNFLYQEQAIEPGILRI
                     GNSFTFREMRGYTILIETQINEGETIRTLASADFSLTGSEPIEIPENISPAFIDAYKA
                     LADNFQTSYLRNLPLSRPKMLIISHTNLANYQTEFIKWKKSQGFEVYVAYKSDIGNTV
                     NDFKQFIQNHYYQYHCDFLLLLGDVTNQGAYSIPTAFYPSPEYQENDADDNQYTFIEG
                     NDYFPEMLVGRLSFNDIAEFMAMSNKSVIYEKTPYMSNTNWMKRGLAVAGNYAEGGLR
                     PSTPVEMSRWLRDKMLNFGYTTVDTVFYPPTYPGTSNIITAINQGVQFVSYRGWGDAN
                     GWHYPAFHIPDLATTQNGPRMPVVFSIVCNTGDFANPVNPSFGEKWMRMGSVAQPNGC
                     VAFVGPSDLHTKTRLNNSIASGAFRSIFDYGVRCFATSVLMGKIELYKNFPNDIGDNQ
                     YVAFYFHVYNIQADPSLKMWVLVPDTISESVIEGGLTFAQSDSHIRINAPHLNGAMVT
                     GTKNGTDFTYSQVIDGFAILPIDPEATGNLTITITKENYVPLIRTLTCSEPAQIGIED
                     NSVEGSILNPNTEITAQITLKNYSDSVYTVNSLSITSTDDATVDYNIQPFTLNPNETH
                     NISFNIIGNPSIRPRKPITFSLSLPEHNILHQFQILGGGAEFTVLNSSGFIAIGNISP
                     VSFQIQNIGTAPMTDASIEIISLTDAADFPNPIQHIGNIAVNEPIIINTTIQTGAETW
                     NGRNIPLKMVLTDNGYSTTVYYAVTVGTPEPNSPTGPDEYGYFAYDNFDIGFDEAPTY
                     NWIETDPLLGGQGTIWEVRDDGSHTIDLPFTFRFYGRDYNQLTMCSNGWISFISTQMH
                     DFYNCYIPAALGPYTMVAGYWDDLKGMKIGIDEQGNGIFNNMRLSWWYDTANNRFIIQ
                     WNDAYNQYNIDLMENASLEKFQIILYPQPDRDGDIVIQYHTVDNPGVTTNYCTVGIEN
                     HLQTVGLTYTHANTYPPTAHTLQPGLAIKFTTIPPDPYTGNNDALQIPFQLAQNYPNP
                     FNPRTTISFSVAKSGYAKLSVYNSRGELIRTLLNDNICSGNYSIDWDGNDDKGKSVSS
                     GLYLYKLEVNGINQIKKMLLLK"
     gene            12632..13231
                     /locus_tag="PK015_01725"
     CDS             12632..13231
                     /locus_tag="PK015_01725"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HOL33227.1"
                     /translation="MSDQLQELLQRVYEEGVNKAKAEADQILEKAKSEAAEIVQKAQA
                     EADRIISEARKQAEDLKRNTESDLQMAAQNTLNAVKQKLTDIFLDEAFNTKLQESFSD
                     PQFLQKLILETVAAWKESGGTIIISQNMEDKLDKFFLQSLSEKASKGLKVEFSPQMKS
                     GFALTPSDGSYKLSFTDEDFANLFKSYLRPRSNKILFKS"
     gene            13239..14126
                     /locus_tag="PK015_01730"
     CDS             13239..14126
                     /locus_tag="PK015_01730"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HOL33228.1"
                     /translation="MHTQYYYFVTGLPVITIDDSKLPFTPDEFLLDAQEHITASDFKL
                     LRWLRLPEKVIELVNVIYNKEDKLHPIDEESLIFYKEYIDHMKERASEPTIPVRKEFK
                     IFPDFLHKIITELYMEEELPPYQKVRHKLLEATYEFCSKSSDDFLKQWFNFNRDMQNI
                     IIAINGRAHNIDYVPYLIGEGELVEKLAKVHTMDFGLGKDNELFESISRIWEQNNILY
                     REKHYDILRWKWIDDFNFFNYFNIERILGFDAQLRMLNRWIKLDPVKGKEIFESTLDT
                     LTKSFSFPEQFKVKSISKK"
     gene            14139..15893
                     /locus_tag="PK015_01735"
     CDS             14139..15893
                     /locus_tag="PK015_01735"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOL33229.1"
                     /translation="MTKGTVKGIISNLVYVEVDGPVSQNEICYIDFQGVKLMAEVIKV
                     AGNIAYAQVFESTRGLKPGDLVEFTDKMLQVKLGPGMLSKNYDGLQDDLNKMEGLFLK
                     RGEHTDPLDEERIWHFTPIAKVGDTVLAGDWLGSVPENWLDHKIMVPFLMEGKYQVKS
                     IVPEGDYKITDTIAVVVDKEGKEYPLTMVQYWPVKLPIKAYQSKPRPFKTLETGIRTI
                     DTLNPIAEGGTGFTPGPFGTGKTVLQHSISQNAEADLIVVAACGERANEVVELFTEFP
                     ELDDPRTGRKLIERTIIICNTSNMPVAAREASVYTAMSICEYYRSMGLKVLLLADSTS
                     RWAQALREMSNRMEELPGPDAYPMDLPAIISNFYSRAGYVYLNNGQTGSITFIGAVSP
                     AGGNLKEPVTESTKKAARCFYSLSQDRADSKRYPAVDPIDSYSKYLEYDEIQEFLNQN
                     ISPGWVDKVYKAKDILLRGKEAKDQINILGDDGVPLDYHSRFWKSELIDRIILVQDSF
                     DPIDKSTPMKRQAYMLNLLLNICDTNFDFESYEELQPFYSRVINVLLQMNYQPFEGDK
                     FKEYEAQLESILAERRVK"
     gene            15893..17215
                     /locus_tag="PK015_01740"
     CDS             15893..17215
                     /locus_tag="PK015_01740"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOL33230.1"
                     /translation="MAGTAFQKIYTKLTQITKATCTVQATGVGNEELATVGGRLAQVV
                     KIIDNQVTLQIFEGTEGIGTDAEVIFFGKPPTIKVSDQLAGRFFNAYGEPIDGGPAIE
                     GDEVEIGGPSVNPVRRKDPSELIATGIAGIDLNNTLVTGQKIPFFADPDQPYNQVMAT
                     VALRTKADKIILGGIGLSNDDYLYYKNSFENAGVIDKIIAFINTTDDPPVERLLIPDM
                     ALTAAEYFAIQKNEAVLVLLTDMTLYCDALSIVSNRMDQIPSKDSMPGSLYSDLAKIY
                     EKAVQFPTGGSITIIAVTTISGGDITHAIPDNTGYITEGQLYLRRDTDIGKVIIDPAR
                     SLSRLRNKVIGKRTRADHPQVMSASVRLNADAMNAKTKLENGFDLTDYDQRALKFARD
                     YAEHILAIDVNIDTEEMLDKTWALFRKYFTPEEVGIKDEFMKLYWNKE"
     gene            17212..17811
                     /locus_tag="PK015_01745"
     CDS             17212..17811
                     /locus_tag="PK015_01745"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOL33231.1"
                     /translation="MNLKFQYNKIAQLQLEKQLKIRQNALPTLKNKESALRMEVKRAR
                     DTALDLDGKIRSSINNLEPIMRLWAEFNPDLIKIENVEFLARKIAGVPVPILGNIQFE
                     ISEYDLFSTPSWYLDGIEMLKDIATLSIEREFFWRKMLILEQVRKKTTQKVNLYEKVQ
                     IPLYEDAIRKIKRFLEDEENLSKASQKILKNRISREAAV"
     gene            17808..19601
                     /locus_tag="PK015_01750"
     CDS             17808..19601
                     /locus_tag="PK015_01750"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HOL33232.1"
                     /translation="MIEKMRKYTFVLYHQDYPKFLADLQKLGVVHLVQNTEEKTETLM
                     KNMEQLEEYAEQLRFLKKLKSTAEKTAVPYPAQVLLQKIQQAREEKEKLERLAETLRK
                     QVRELEPWGHFDYNLVKSLQQAGVKVEFYTCLKNHFKPQWQEEYAVKIINEVNGILYF
                     VVLYTDEKAEIEADPFSFHQHTLQELESQLKETEHRIKDIDEYIYNIAPAAIELFSNE
                     IANLSKTCEFEEAVLQGIDEAEGKVKILQGFIPQRLEPELVKFLESENVIYFANDATI
                     EDNPPVLLRNNWFARLFEPISKMYMLPYYNEFDLTPFFAPFFMLFFGFCNADMVYGVL
                     FILIGLILNKKMKNPFIKSISGLAVLFGTSSLIFGLLMGSALGFDLKGTALNPYVLIR
                     DNNQIFNFSLLLGAIQILFGTIINGVKKALQYGLKYFLAPFGTFLFLLSLTILGAGLL
                     GVDNSALTPYTKYPLWIGLALMMLFNNPAKNPLINILSGLWLLYNVVTGFFGDILSYI
                     RLFALGVSSSILGMVINSIGMQFLNIKILGPIIFFIFMAAGHTLNIALGALSGFVHPL
                     RLTFVEFYKNANFQGPGIPYQPFGHKKMIKI"
     gene            19614..20129
                     /locus_tag="PK015_01755"
     CDS             19614..20129
                     /locus_tag="PK015_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HOL33233.1"
                     /translation="MPYILLQAGTAAANALPISGHTTAYILAWLGLFLMVALSGIGSA
                     VGTVIGGSATIAAMKKREDVFANCMILSALPGTQGLYGFGAFFILKGFATADVNMITA
                     AAIFGAGLISGLVNLISAYFQARVVSTGIESIGNGHNVFSNTLILAVYPELYAIISFA
                     ACFLISAMLPL"
     gene            20201..20548
                     /locus_tag="PK015_01760"
     CDS             20201..20548
                     /locus_tag="PK015_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33234.1"
                     /translation="MDYRDSHQTPSGQQFQYWQYPPMQPHRGVLIFIFGLLGLFTCAI
                     FGLLAWIFGNEDLKKMEQGIMDPSGRDLTNAGRILGIISVSITVLGLVIILAYFFFVM
                     GVAASLSNGVFHF"
     gene            20577..20966
                     /locus_tag="PK015_01765"
     CDS             20577..20966
                     /locus_tag="PK015_01765"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HOL33235.1"
                     /translation="MRLRIEKLDTRFPYGKIPLLLFFFLLAIYFIFYLFVPYKIPLCW
                     FKSLTHINCPTCGLYRAFTALLQGHIITAISYNPLMLFLTIFLIIQLICTTLFKIKIS
                     LKAKVKEKHWILFFFIIAFIANWLYII"
     gene            complement(21213..>22419)
                     /locus_tag="PK015_01770"
     CDS             complement(21213..>22419)
                     /locus_tag="PK015_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33236.1"
                     /translation="KIPIPQKYRILAQDCRGNVYVSKAKIKEYSRLEIGNEYTAEEIV
                     TAAKKIWNSQFFKTVYPVLEPYKNGYKLIFYVQEYDRRSIIFNMSYTTEEELNISGAM
                     ELNNLVLKNSKLLTGFTLGGRSELSLDYVKNFGEFWGSYFRIFPYISEKRLYLYDEDF
                     YKISSVKSLEYGITPGVGFFANNLAIAEGFFYSYKTRLYRDVSAVAPVDSLYLISGLG
                     VKVYHESLNDDLFPLSGLRVFSKLNFAPWEQISDRLYNKAVVNVDLYAPVSRTISLRA
                     GYNFGTYFGAREESSLDPFYFTGSQGYRAYPRYAISSPQYCYYTIASVLNPRKNVFLE
                     TGVQSLNYVNEEFWDLNQKLEWSFYADIGYRAYLGPIHFSAAFSREHSPCFYLNVGYN
                     NDLFWFSRK"
     assembly_gap    22420..22519
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<22520..23539)
                     /locus_tag="PK015_01775"
     CDS             complement(<22520..23539)
                     /locus_tag="PK015_01775"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOL33237.1"
                     /translation="MSNNKFYLIILILLLSFSFKPTEAKSLGYAFSGGGARGYAHIGM
                     LKVLAEEGIYPEYIAGTSFGALVGSLYSMGYNPTEIESLTTRWDVLDLFDEHYRRKDL
                     YIGQKRWPSYGNFQLRIKENGQPSYPTGLINGVKLEQALAQVYMPASSYRDFSKLPVS
                     FAGLYLDLDTGELIVSTSGSLMQTIRAAISIPSLFTPFELDGHRYIDAGILQNIPVPQ
                     VREIGADKVITFKVNADLTEKNPDNLIYILNQTIEIAMYQSYESSLALSDLVLEPDLS
                     EFSNMSYDKAKEIIDAGERYARSHIEEIRAFRDSLLAEGYVFKEPQKIPIPQKYRILA
                     QDCRGN"
     gene            complement(23570..24058)
                     /locus_tag="PK015_01780"
     CDS             complement(23570..24058)
                     /locus_tag="PK015_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33238.1"
                     /translation="MNILFGHLFWGILIILIGVSLILKGFNISFPIFKIFIAIIIILL
                     GVKLLIGGWGNTKHKETPTKLYNATEREYNAIFAAQKLDLTNIEPDASPLEINAVFGS
                     FVVELPDDINFDFSTTAFCGSIDIPKKTAMDEAKTRGTVKIDANAVFGKITFIISTHH
                     SS"
     gene            complement(24075..26285)
                     /gene="purL"
                     /locus_tag="PK015_01785"
     CDS             complement(24075..26285)
                     /gene="purL"
                     /locus_tag="PK015_01785"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HOL33239.1"
                     /translation="MEPPITRELIASHGILETEYQYILEILKRNPTYLELGIFSVMWS
                     EHASYKNSINLIKTLPREGKYLLAKAGEENAGVVDIGDGLAICFKIESHNHPSALEPY
                     HGAATGVGGILRDIFTMGARPVAVLDSLRFGDLENPRVMHLLKEVVHGISDYGNCFGV
                     PTVGGEVFFDEAYEGNPLVNVMAVGVAKHEHLAKSSAKGIGNLVLYVGAKTGRDGIHG
                     ATFASVELSDQSASQRTAVQVGDPFYEKLLLEATLEVIQAGLVVAIQDMGAAGLTCSS
                     SEMAGKGGVGIELELEKVPVREKGMTPYEIMLSESQERMLMIVEPENLEEVQKVFQKW
                     DLDAVVIGKVTDDKMLRVKLNEQVLAEIPAEYLVLGGKAPVYTREAKKPSYIDEKKSM
                     DISSLPEPENYTSALYRLISEPSIASKKSIFSQYDYMVQIATKIEPGSDAAVMVIKGT
                     DKAIALSTDCNARFCFLDPYEGAKAAVAEAALNVACSGGRPIAITNCLNFGNPYKPEV
                     FWTFSEAIRGMKDACITLETPVTGGNVSFYNENPEGAIYPTPVIGMLGLLENYKKATT
                     QWFKHSGDIIAFLGRTSSELGASQYLKTIFGTVAGKIPSLDLKFIKRLIEVLLNLIEE
                     DIISSAHNVSEGGLLVALAECCFQPENLTGAELNFPLTFSKSVEYFNESIERVVISFN
                     AENLTKVQTVCDKYDIPLKLLGTVSDKPMFKVNQDIYVSTEALYKAYQSGIKIN"
     gene            complement(26289..27416)
                     /locus_tag="PK015_01790"
     CDS             complement(26289..27416)
                     /locus_tag="PK015_01790"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HOL33240.1"
                     /translation="MVILIRPSLRKLEKLASTGAFDNKTILHYTGDMFGIGCKVSSYE
                     AIERIQKLKGRSNKHSFILLVSSLQWFEKEGIYIPDRLISLLEQYWPGNLTVIFKCED
                     KRFAHIAVDGKVAFRVPDDELLRVFIDILEEPIISTSVNISSLPPESDLQRLTTFYAD
                     WFDYAILPGNKNYPYNSQPSTIVEYISSREEKNQSGFDELKCIREGSIPFYAVKKSFE
                     KPTILFVCTANICRSPIAEKLFNHYASRINLPYSADSAGILPGGQSISTASMQLLLQR
                     GILEARDHISKQITPEMIQGSYLVLTMEERQRDFLRMQEPKQVHKIFTLNEYVGETGD
                     IADPFGADLDTYRETFNIIDDRIQRLIEKLKDTNFIEKDES"
     gene            complement(27433..28542)
                     /locus_tag="PK015_01795"
     CDS             complement(27433..28542)
                     /locus_tag="PK015_01795"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOL33241.1"
                     /translation="MRKKILCFLSLFLIFSLLIGDEISDTQKRLEKIQKDLQEMEKKA
                     AQTSQKKKTTDVQLQTTLKEKQKVELEKKKTEEVVKQKQQVLTKVSTELKTVEEQVRE
                     LKTLQNNQLNSLLRIRINSSTTKVKPKEERYLTVMTAQTRQKLNQLGDVQVVLAVEKQ
                     EVGKEVKLAESDLYAKANLSKKLNTTVKTLETQKKQLTKEEQKLQNQIAQLRKDAAQL
                     ESLIASLTAKSKASGEISEAYKTSFKSIAWPLRGKIIRNYGEETRAYGTSVISNGIDI
                     AVPEWTTVVAADDGEVVFSGPYGGQGKLIIIDHKNGFFTVYSYNNELLVSRGAQVKKG
                     QPIAKSGMTGSASEPCLHFEVRKDGKAVNPLAYLE"
     gene            complement(28689..29654)
                     /gene="secF"
                     /locus_tag="PK015_01800"
     CDS             complement(28689..29654)
                     /gene="secF"
                     /locus_tag="PK015_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOL33242.1"
                     /translation="MRLFQNANYPFVEKRYIFFVISAVLVVASIIGIIVMGINWSTDF
                     TGGVSLIINMQPKDPNVQPLSIDNLRKVINEAGFREAEIQFIGDPSNATFQIKVGGED
                     VNKIKDQITEVLATKLPQYVQGRDLQTDVIRQIDVVGAKAGMEMRNNAIMAVLIAMLF
                     MIIYIWIRYEFTFGLMGILAIFHDVIILVGVFAFTQKEFTMTILAALLTLVGYSINDT
                     IVIYDRIREDLKIYRKDPVPLIFNRAMNKTLSRTVITSGTTLLTSLALYFFGGPVIHD
                     FALAMSLGIIIGTYSSIFIASNLVLETLVHTHTEKKALKQLTKKK"
     gene            complement(29666..31255)
                     /gene="secD"
                     /locus_tag="PK015_01805"
     CDS             complement(29666..31255)
                     /gene="secD"
                     /locus_tag="PK015_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOL33243.1"
                     /translation="MRKNSWLTPVSILIFVLGTLIFLSPLFVPEDVNWPIKQKLKLGL
                     DLRGGTQIVLTVVTKDLPEADKAAAVENNIRIIRNRIDQFGIAEPTIQKMGESDILVQ
                     LPGVKDPIAAENLIMKSAVLEFKLVAEPDIAEKFINQVDAIIQENLDKFPVLAKLAEE
                     DKALAKAGADSVKTGEGIFKSLITTTERDFAVRYEDLDIVRSLLQDSTFVNLKPKGYD
                     LVLESVDEKKKASREPVVLYVLESTPQLKGTDVDRASMEIGPSDSPNPQIANKPYISL
                     TFTRTGARKFANCTGDNIGKRLAIVLDNTVYSAPVIQDRIPDGKAMITGQFTEQEAKS
                     LAILLNNESLSAPIEPKYSTQVSATLGEDSIKSGFRAGLIGILAVIIFMALYYKGAGL
                     IADFVLIFNVGFILAAMTIFGGTLTMPGIAGIILTIGMAVDANVLIFERIREELDAGR
                     SPRSALDAGFKRATITILDSNITTLIAALVLYQFGTGPVRGFAVTLSIGIVGSMFCAI
                     VFARYIMEKTMIKPGKNTMSI"
     gene            31751..35326
                     /locus_tag="PK015_01810"
     CDS             31751..35326
                     /locus_tag="PK015_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOL33244.1"
                     /translation="MKKLILVLTLLAGIIYLSANVIYTYNLAAPEIKTEGNYIKVNLS
                     GAQSYGNAGAPDLPWIGSKLLLPMGEEAVDIQINLSGAKDIVLDKKIAPIQPPIKLSE
                     IAIAQRIDPDPAIYDRSVAYPEQKHNGSNTQYFAGHPINFTAICPFDYNPVENKLTFY
                     HQITITIITSPSSKAVEAKTLLKQDVDTADRLKKIIDNSQAIPRYEERLIGYDYLIIY
                     DQAKQSQWLPFKTLYEDRGLNVYMKPIQEIVAQNAGIDTQEKIRNYIISFYSQQPLKY
                     VLLAGDTDVIPHRGFYVNMGSGGEVDADIPADMYYSCLDGNWNTDNDNYWGEPMESDL
                     VPELSIGRFCYNSDDEIANFINKTSMYLNNPVIPEIKSAFYVGEYLWEGPTWGGDYMD
                     EMIGGSSANGYTTVGIPVNWNITTLYDRTYGSSNSWGASQIRPLLSQGPNFVNHLGHS
                     NTTYAMRLAVNQVTPTTITNNGVNHNFSIIFTQGCYAGAFDNRETDVGSYTVDSITEK
                     FTSISTAAVAMIAHSRYGWGVVGSTDGASQHIHRQFIDAIFGEGIYQLGKALDDSKID
                     NIPWITNTPVMYWVTYETNVIGDPALMAWTDTPQQIIAELPSYWIVGLNYYQIQTNAP
                     NALFRIKNGENLICETTADASGMITVTFLGNLTPGTIDVYIIAPNFLPFHQQVVVEAS
                     NMPYIVPTTIALIDDDGLYHTGEEININATIKNVGLMDQTGQGTITLLSNSSNVLVLN
                     GSAQFNPLAVADSTVIAGIFRIRIQGYFPDHQIANLNFLTTFDDYSVTTPFIITLNTP
                     ELNIDSYNFVNDCPLIMPGDQPYLNMLVSNSGSGNAYTPMLILFSDNPNIILSESEVL
                     LPPVSAGNSVFCENIFSVYISDAISAGTAINIGYMLSAENGNTIEGNFVLYVGNQHYT
                     YENDFTGWDVLAPLPNFINQWHRENYRNHTTNGSWSMKFGGIGNLNYANSAYGVLESP
                     ELPLVLNAQLHFFHWMDAENHTTNPQYAWDGGLVQMSLNGGEWTQITPIGGYPYKIYS
                     NVASPFPAETRVYSGTFDWTEAVFDLSGYTGTAKFRFIFGSDGAVTKEGWYIDDLYME
                     NEVPNSDLVETIHFELYENYPNPFNPTTTISFNLPKNAPVNLEIFNLKGQKVRTLINS
                     ELKAGKHNIVWNGLDNNGKPVASGVYFYRLSNGEQILSRKMMLMK"
     gene            35326..36045
                     /locus_tag="PK015_01815"
     CDS             35326..36045
                     /locus_tag="PK015_01815"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HOL33245.1"
                     /translation="MKVTKDTRVLVLTGAGISAESGLKTFRDAGGLWENHRVEDVATP
                     EAFRRAPVMVWNFYKERWQQALQAKPNPGHLALAKLENWLQDNFHLITQNVDGLHTAA
                     GNKRVIEMHGSLHTCFCTRCHTRFPMKDINLEEDIPLCPKCKGMLRPDVVWFGEVPYH
                     LDKIDALLHNCDVFMVVGTSGVVYPAAGFVMTARYFGAHTIAVNLNPMENSEFIDEFY
                     QGKAGEILPRLIDEWINSADK"
     gene            complement(36069..37016)
                     /gene="arcC"
                     /locus_tag="PK015_01820"
     CDS             complement(36069..37016)
                     /gene="arcC"
                     /locus_tag="PK015_01820"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOL33246.1"
                     /translation="MDKIALLALGGNAIIKAGEKGTITEQFANTRASLGGVVELIRQG
                     YRLAITHGNGPQVGNLLRQQELGEKEGIPPLPLGVLNAATEGTMGYMIEQSLQNRLLL
                     SGIERDVITIISQVIVDKNDPSMLNPTKFVGSTYYTQEEAKELEQKLGWKLREDSGKG
                     YRRVVPSPLPQRIIPAETIKEMVHEGKIVIAVGGGGIPVYIEEDGTYEGVDAVIDKDF
                     ASSLLALNIEANLFVILTGVEKVAINFGKPDQKDLDYLTIKEAQRYYNENQFPAGSMG
                     PKIKAAIDFLERGGQEVLITSIEKIVPAFSGKTGTRIIH"
     gene            complement(37100..38413)
                     /locus_tag="PK015_01825"
     CDS             complement(37100..38413)
                     /locus_tag="PK015_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HOL33247.1"
                     /translation="MKRRVVIMGAAGRDFHNFNVFFRDNEEYEVVAFTATQIPGIDDK
                     KYPAELAGRLYPDGIKIYSETELPELIKNLNVDLVVFAYSDQAHEVVMHKASLVNACG
                     ADFMLMGTKNTMIKTSKPLITVCAVRTGCGKSQTTRAIVKALKAKGRNVVSIRHPMPY
                     GDLVKQKVQRFAKLEDLDFHHCTIEEREEYEPHIKMNSIIYAGVDYEAILREAEKEAD
                     IIVWDGGNNDFPFYYSDKQLNIVVVDPHRPGDEISYHPGETNLYRADVVIINKIDSAD
                     LDDINEVRANVRAINPRAKIIEAASPLMVDHPELITGKRVLVVEDGPTLTHGEMTYGA
                     GVVAADKYGCEDLVDPREWAVGEIAETYEKYPGIGILLPAMGYSDRQIKDLEKTINDA
                     ECDSVVIATPIDLRRIINIKHPACQVTYELQEIGRPNLEDVLQDY"
     gene            39323..41902
                     /locus_tag="PK015_01830"
     CDS             39323..41902
                     /locus_tag="PK015_01830"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOL33248.1"
                     /translation="MSLSFLYGIGSESKTNSFDSGDFPKYIDYILYPDSGPSINNVLY
                     EIESRFNNNEKDHFTVLLSNGIYNQSFYFELCSFSSAFSDLYIEIRGSSKNNVQIQGG
                     NYINTYGAANLHFKLTNLSINNGFRGIEAYTDYENNYITFLEIDNCSIHDNISQSFGI
                     DVCMYGAGIYSNGPTVITNCDIYNNKGKNWIHPAYLNYSRGGGIAIVNNTIYNSEITD
                     CNIYNNEANAGGGIYVSGTAPITIARNRIHGNTRKLYNYVTAGFGEGVYCYDCHKLTF
                     KDNIVYEQIPASYEKDPREPIPIEAAVVIESCGVSPLIASIKIENNSIMDNDECHGLW
                     VMMPEGGTVIRNNLSCRNLSGIYLSNYSSGYLTLEYNDAYDNGYLNQGEILNYFIQYP
                     TEIVSFNNYELDPQIDTSYMPIWDSSTISWIIDAGDPALTDSDGTRSDIGAIRAGEHY
                     YEEYSMPVNGGIKWMSFPVLNTVTEGYTTNSNFFAPILYQNILDWVDWKKEDNQQLRM
                     QIIDGYVHNGDGTVSSPVGYKVKVKDGVSNQIKIITTGYKESDLKQIHLYKYLSGTNI
                     ENENWIGYFQKESAYALDAFAPVLDKILMIKAQYWSMARDPITGIWKVTQEDLTINYG
                     DMVIVTVSEDCSFIWNNKQPVDPKYRELPEHFEYIEKEDYTPLYLDLTSLEELPSEIG
                     VYVNNECKGAVKVAGDSTDICVYIGANETLNPDNCELILYYDTKGIQPVRMVYQPEAG
                     ELIQLSNGGIRFYTLKLKGSPNLKPVIPVTRLLQNYPNPFNPSTTIVYEIGEEGPICM
                     EIYNIRGQKVKTLVDGFKVAGMHRVVWDGRDDNGRRVASGIYQYRLTTRDGSITKEML
                     LLK"
     gene            41967..44420
                     /locus_tag="PK015_01835"
     CDS             41967..44420
                     /locus_tag="PK015_01835"
                     /inference="COORDINATES: protein motif:HMM:NF019236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HOL33249.1"
                     /translation="MRKRVWLVLCISVMIVTFSATTLRVALDGSQVYQSIQSAIEDAV
                     AGDTVLVYPGRYYENVEFMGKSISVCSLEAATGVSTYISTTIIDGGMQDPCIAIRADE
                     LEATVRGFTLTNGLGFLEWQTDNYRCGGGIYIYRDFQSPYSKQVNVINCDITENRAVW
                     GAGIYCIESSLFLSGVNIHHNYASLTAGGINFYGTIHKSPNIVFDPVNLCSVYANYGR
                     NPVDILITDTIGDTYINLDLASVLHPDVFYIDRIKNLSIYYPYNNILNIQRGYRTEIN
                     SDFYVSPTGDNANTGLSPDQALKSIAEAVQRIASDSLEAKTVYVLPGTYNEDESFYLF
                     PIPLKSYVNIIGAGSDKTILHSDIASPTGNTLFWGGQNLQHITIHGFTTSAEINQNCR
                     PMISFYISHLTISDMILEDFPIYNYRDAAIFLYEPTSLSIDSLVIRNITVPEHAVYIH
                     NTLQDCSITNSTFENIHSTYTSPDTPGDDSWGPTVVEIITLDTLTVDNCIFRNISVQN
                     NQATLYIGNNVYFPGNVGNLKVTNCLFDNIRTNYISGVCFITNIYGSFKVSNCTFYNN
                     FGTLGAVMAKGKIDMRNNIFYNPDAQYELYMGVPDLDLYSVTRLDFDYNDIRNGYNGI
                     RNSSPTNDLYYREHNLSSEPLFASLTYGDSLYLRLGEGSPCINAGTPDTTGLGLLPYD
                     LAGNWRIWDGCIDMGCYEYGSEPYVGVEDPVLPSIPGYNILAYPNPFNALTNLKVKLP
                     SDSNNTFNKVNSASIEVYNIRGEKVKTINLDVSKGAEQWTYWDGRDQHSKLCPSGIYL
                     LNLKLNGKSVLTRKVTLIR"
     gene            44504..46957
                     /locus_tag="PK015_01840"
     CDS             44504..46957
                     /locus_tag="PK015_01840"
                     /inference="COORDINATES: protein motif:HMM:NF019236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HOL33250.1"
                     /translation="MRKRVWLVLCISVMIVTFSATTLRVALDGSQVYQSIQSAIEDAV
                     AGDTVLVYPGRYYENVEFMGKSISVCSLEAATGVSTYISTTIIDGGMQDPCIAIRADE
                     LEATVRGFTLTNGLGFLEWQTDNYRCGGGIYIYRDFQSPYSKQVNVINCDITENRAVW
                     GAGIYCIESSLFLSGVNIHHNYASLTAGGINFYGTIHKSPNIVFDPVNLCSVYANYGR
                     NPVDILITDTIGDTYINLDLASVLHPDVFYIDRIKNLSIYYPYNNILNIQRGYRTEIN
                     SDFYVSPTGDNANTGLSPDQALKSIAEAVQRIASDSLEAKTVYVLPGTYNEDESFYLF
                     PIPLKSYVNIIGAGSDKTILHSDIASPTGNTLFWGGQNLQHITIHGFTTSAEINQNCR
                     PMISFYISHLTISDMILEDFPIYNYRDAAIFLYEPTSLSIDSLVIRNITVPEHAVYIH
                     NTLQDCSITNSTFENIHSTYTSPDTPGDDSWGPTVVEIITLDTLTVDNCIFRNISVQN
                     NQATLYIGNNVYFPGNVGNLKVTNCLFDNIRTNYISGVCFITNIYGSFKVSNCTFYNN
                     FGTLGAVMAKGKIDMRNNIFYNPDAQYELYMGVPDLDLYSVTRLDFDYNDIRNGYNGI
                     RNSSPTNDLYYREHNLSSEPLFASLTYGDSLYLRLGEGSPCINAGTPDTTGLGLLPYD
                     LAGNWRIWDGCIDMGCYEYGSEPYVGVEDPVLPSIPGYNILAYPNPFNALTNLKVKLP
                     SDSNNTFNKVNSASIEVYNIRGEKVKTINLDVSKGAEQWTYWDGRDQHSKLCPSGIYL
                     LNLKLNGKSVLTRKVTLIR"
     gene            complement(47234..49291)
                     /gene="asnB"
                     /locus_tag="PK015_01845"
     CDS             complement(47234..49291)
                     /gene="asnB"
                     /locus_tag="PK015_01845"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOL33251.1"
                     /translation="MCGISGIYSFDTQKVIDISLLKAMNALIEHRGPDDEGFCLIEKN
                     SHKILPFSGDGSKEEIRQLYPSLNLSARASLGLGFRRLAILDLSMKGHQPMLDPQLNL
                     LIVFNGEIYNYIELRQELIAQGYSFNSESDTEVILKAYDFWGENCVLHFNGIWAFALW
                     DANKSLLFCSRDRFGVKPFYYCRNRDFLYFASEIKQLLLTPVDKSLNLPMIWRSMKIN
                     SLEVYGEETFWQEIKALQPGYNLIVDQHGITLKEYYKLNPASFGKSSLSLPEAIEIYR
                     ETFEKAVQLQMRSDVEVGSCLSGGLDSSAIVCTASRLTDRPLQTFSAYFPEYPPLDER
                     KWIAEVVHSSGCISHLTSPQAEDAMAWFNDVTWFNDLPLGSGFAAQYAVMKLAQENGI
                     KVLLDGQGSDELTAGYRHSFYRFLADLILQTKFHKALKEALAWLKKYGCIKGLSGLAK
                     ALLSIFLSEPQLYNFEFRFLRFEPFNQDFLQQCKKSLSEPILAQISDFSSKSVSKFFS
                     QHPIKLNNTRTLTSDNFSKPTCDKLSNFLYNAVYTTSLPTLLHWEDRMSMSASIESRV
                     PFLDHNLVELAFSLPSEYKIHNAQGKYIHRQAIKPLVPTAIFNRKEKSVFGSPFISLW
                     LRGPMKTSYESLITSSTFRNRGIWNLPLIMDNWQKYLKGNNSQAEMLYNIYALEMWFR
                     RFS"
     gene            complement(49364..>51039)
                     /locus_tag="PK015_01850"
     CDS             complement(49364..>51039)
                     /locus_tag="PK015_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33252.1"
                     /translation="RFGSEFILYQTNPPDYPDDYIWVKFRFRISNLQPGISSNTPLLR
                     FYVTGFELYGTGFSSQMKILNHWIDNQQRSETIFTVHNYLLNRKGNEFLELELKIPYK
                     DLIDANLLTADIDHNPATSDSREFLRLVNLNPRVYWYGNCDVELDYVEIEDELHHKIS
                     HDKNFWQDKILQRMYNVISQGEGNVKGFYTFDEPYQGQFDSFKLMQEIASQEDIPVFT
                     AVYDFQVTNITLNKEQGIYYDHIDAFSKIAQPQIIAPDIYPLKPDLIWNATEGEKGKF
                     IQYILDQKLLSVYQDCMEYRDKKEGRKFYPIVQVLGKWTLYQGQEQWVDWIQPTTAAQ
                     KVLLYLPLCFKPDGIFHYCLRSYQDIKGYGQRSIAFSRVGMPDYPLLVPDPITWRAVS
                     LSNPRIKAYGVIIKDLNWQNSECIGTSRKKFKKAEKDNPIQYIQVQKQGIGEYEGYIQ
                     CATYLDKEENLWLMVVNRRANFFLPGIITEPQFVPPEEFDIYFPEAPPQKLLLTFKDS
                     GRKNPYQNYAFYDPYEDKFYPYHNGNIEIELPAGEGRLLKLVNRTSNDR"
BASE COUNT        16365 a         9021 c         9340 g        16213 t
ORIGIN      
        1 ttacttggtg caggtatcat aaccagctat ctttattctc tttatctatt gacctctacc
       61 ccatttacta aatcagatgg tttatcattt ttctggacga gtatcatctt atttgttttt
      121 gtcctttttg gaatttcact ggctgatatg tttattgtgc gttatcaaac cagatggata
      181 aaagcagaac aaaaagaaac tgaggagagc aaaaatcaag atgattgaag accgagattt
      241 cttcgttctg gacaatgtgc aggaaagaat tgaggttcct gcattgtttg ggaacgataa
      301 acaactttat atagagattg gctcaggtaa gggagagttc atttctcaat atgctctttt
      361 acatccagaa tggaatttcc tgggttttga agcacgagaa aaaaggattc gtaatatctt
      421 aaaaaaactc tctccttcca cacatccaaa tgttagattg gtaagaatgc tggtcaattc
      481 taacatcgct tctcttttac ctccagaatc tgttcaggga gttttcattc aatatcctga
      541 cccttggcct aaaaaaagac atcataaacg tcgtttaatt caacaagatt ttctgaacgc
      601 tcttgctaca attatggttc cagatgcaga agtgcatatt tctacggatg acctggatta
      661 tgctaattgg attgccgagg aatttctcaa aaatccatat tttatctctg tgcaggatga
      721 aattattcaa aaaatcccca gtctggaaga ccatattccc agctggtatg aagcaaaaca
      781 aacccataag ggcatcagtc caaattatat gctgtttaaa cggatataat taattatgat
      841 ttattgtata aaaattttat tgactacaag ataactaaat agaagctatg aaatcaaata
      901 gcttttttta ttaataaagt ataaaggtat taacttaaat gaataagaat attgaattac
      961 aacaactctc tatttttgat aattattcct atccttctta tgaagaaata ttagaggaat
     1021 attcaaagga attcgaaaat tatgttctcc ctaaaggaga caccatattt ggtttctgga
     1081 tgcaaacctt agctgattta gaattccttg atttagaatt gcaagggttg actgaagatt
     1141 atacaattga tcccataaat agaaaagtaa aatttagtgg agatgaagaa tttattagat
     1201 taagaattgc acatttagaa aaagtaaaaa acaaacctac tttgtataca gattttgttg
     1261 acaaatttgg agatactaat gcctatgctt ttcacaatct ttacccatat aagggtaaat
     1321 tttatccgag aatagtaagg acattaatca atacttttaa tttaaatgaa aattctttat
     1381 tagtcgaccc ttttaatggt tcgggaacta caacccatga atcatctctt atgggaatta
     1441 aaagcatggg aattgatgta accccaattg gaatagtatt atcacaatta aaaaatgatc
     1501 tactttttac tgatgaaaat aaactcaatt tttctgtaaa ggaactacaa gaaattcttg
     1561 aagttattaa aaataaaaaa tggcaacaca atgatcctct catatataaa ctaatgttga
     1621 tagtatattt tgatactatt gatgcattta aaagaacttc cagatataat agaaaaggac
     1681 aaataggctt atttatagaa aaatataatt atataaaaga atgccatact aaaataagag
     1741 atataaaaaa tgaatataat ttaatattcg aacgtgctca gataattgat ggtgatattc
     1801 tcgaactaaa gaatatgtat gatctagaag ggaaatttga tgcctgtatt actagtcctc
     1861 cttattattt ttctatcgat tacgtaggta aagacaaaat agcttatgat tatcttgatg
     1921 tagatatgaa aaaggttgaa tcaaaatatt taggaatgaa aagtaatggc aattctaaga
     1981 gcatttatag tggatttcct attagggttg caaaatatta tgaggattta aaaacttcta
     2041 ttgaaaatat atattggatg ttaaaattgg gaggtcgctt agcaattata ataggtgata
     2101 gtactgtaaa tgggaaaaaa atcccaacta ccatgattac taaaaagttt tgtgaagaaa
     2161 taggttttaa atttgaaaaa cttatattta atcccttatt aggagctcgt aatagagcca
     2221 taagaggtga gagcgtaata atttgtatta aggaataatt atgattacaa aaagaaatct
     2281 tatctgtgtt aaaaataaag aaaagttaga acttggccat ttattacttt acgaacctta
     2341 taaaaatata ctggaaaact ttaaaaaatt atgtatagat attaaagaaa aaagttttga
     2401 tcctgtagca aaagtgtatg atggtttatt aagtgttcct aatgaaacta gagaatatta
     2461 tgaggcatta ttaggtgtaa gttcttatta ccaacatagc caaggtggta gaggaaaata
     2521 cattgaaaag aaaattgctt catcttttga aacctgtaca ctagacatta gcttaagtaa
     2581 aattccttat tggcttactt acccttcact acataaaaaa cagggtttat ttactcaaag
     2641 agcattgtct agagaagaaa aagagaagtt aagaacaatt gaatgggagt ggttgggtga
     2701 tagagatgta aatacagatg tgggaagtat tatatctgaa gaaaatacta ttgtcctggt
     2761 tgaaataaaa aatagagtgg atagtggtgg aacagcggct agaagagaaa tatggacatc
     2821 tgaaaaattt ggtttatact tcgattatct tataaataat gaaaaaatat ttcaaaaaaa
     2881 tttaaataaa tattcactat cagagttact tgaaagtttt ggaattaata atttagaaat
     2941 atatattgga atattatttg atacaaacga tagcacagca actatagaaa gtgatagaat
     3001 aaaagggttc tattcttcca gtaaacaagg ttttgaatat cttaaaaata aaataagcca
     3061 gaattcatct atttatatta ttaatgaaga tttagataac ctctttatgg aacttggttt
     3121 gagttattct aaattaaaaa taaaaattgg agctttatat ggtgatgata taactttaaa
     3181 actctttagg caaaagttcc cagtgtctga tttgctttta ttaaaatacg atgatatttg
     3241 gctttcacaa cttatatcaa tagatgaaag aacaatactt ttaaaatata atatgaatta
     3301 tacaaaaatt tttattgatt tattggacag gagtaatgat ttaagaaaga aatatgatat
     3361 tttgataaaa tctgaatgcc aagaatcaga actatttgaa attgttaatt atcttttaac
     3421 aaattataac gataaattta atgataaatt cttacctcct ggaaaggaaa aatcttatta
     3481 tttaggggat ttaatccaat ttttatgtgc agttgacgca taactttctt taatacacaa
     3541 caatatgatt caaatacagc atatttataa tagaataaat atagaaaata attttattaa
     3601 aaaaaaagaa atgtaattta tacttattat cattcactaa atttaattaa aatgcaactt
     3661 agtatattaa tcatgattaa gctatttttt actaactctt agtatagttg cttattatta
     3721 tgcgacaact tgtaatacta acaaatcata aactttggtt agctccaatg gctggataca
     3781 ctgatagagc tttccgtcat ctttgtaaag aatacggtgc agatgttatg gttagtgaaa
     3841 tggttagtgc tgatggactt atccgaaaca caaaaaagac ccttccctat atcttatttg
     3901 atggcttgga acgccctttc ggtatccaaa tctttggttc agaaccatat attatggcta
     3961 aagcagcaga actatgctta gcctggaatc cagattttat tgatttgaat atgggctgtc
     4021 cagccaagaa agtggttcgt aaaggtgcag gagcagcgtt aatgaaaaat cctgtgctgg
     4081 ctgcagaaat agtgcgtgaa gtgaaagcag ttcttgataa aggatgtttt ttaagcgcta
     4141 aattccgtag tggttgggac cttaaatcta taaattatct ggattttggc ttaaggttac
     4201 aagatgctgg agccgatttc ctgtgtttac atcccagaac tcaatcacaa atgttttctg
     4261 gaactgctaa ttggacacat attgctgaac tgaaaaacca actttctatc ccactaatag
     4321 gcaatggaga tattcaaact ccagaagatg cactgcgaat gtatgaagag acaagatgtg
     4381 attctgttat gattggtaga ggtgctttgg gtaaaccctg gatttttcat caaatacatc
     4441 agctggaaaa aaacaatgat tacactcctg caagtcgtca attaatcctt gaaactatgc
     4501 tgcgtcatat tgacctggca actcatttta aacctgaatc tgtggcagtt aaagagttaa
     4561 gatctcagct ctgtttttac actaaaggta ttatcggtgg agcagaatta agacaggcta
     4621 ttaatcacgc tgtaagtgcg gaacaaatta aggaattgat taaaagttgt gaaggactga
     4681 aattttagta gagttatttc tttttgcgat acttatatta tttgctcaat tatttttata
     4741 tatcaatatt attaactctc ccttagctct ctcttggctc ttgcttaact gcggaatcaa
     4801 gaaaaagcta agagggacac aggatagagg caagaaagaa cttattgaaa atacttagtt
     4861 aataaaattt taatggatac tgtttatata tggttaggaa ttaagtaaaa taaattaagt
     4921 gccaaattga gggaaaatga tattgatttc tcgttcggca ctttctttgc tatctgaggc
     4981 atgaactgca ttttccgtta ttccttctgc aaaaaaatcc cggatagtgc ctactttcct
     5041 tttttctgga ttaacatcac ctattaaatc tctcaaatct tcaatggcat tttccttttc
     5101 cacgattatt cccacacagg gtgcagaaca cataaaatta accaggcggt cataaaaatt
     5161 cttcccttta tgcatctcat aaaacacact ggctaattca ggagtaaatt ggaaagtttg
     5221 aaggtatttg agattaaaac cagcctgctc aaccattgta atgatatgcc ctatatgatg
     5281 atggaccact gcattaggtt taataattag caggctttgc tcaatcatct ggctttatag
     5341 acagtttcta tataatccag agcagcaact tttatagcaa tatcacggtt tagaacctga
     5401 ctcattttaa ttttatgtat tagaatttca gtccagattt cataaatttc attgggcttg
     5461 aaatctatag cggtatacat tttaaccatt tccgtgtaga agccatctct atcagaatcc
     5521 actcttccga taaccagttt aaagctatca ttacagacat catattcttt gtaacgcaca
     5581 tcaatgacag cataaccaag cttcagtaaa ttatcataat taacttgaaa atcttccata
     5641 tttggcattt tctaactccc tataaatttc tcgacaaaaa gcaggaactc tgcaattatg
     5701 tatccataat ttgacagaaa tggtaaaagt caagcaaaaa ctaaaatgaa actttctgcc
     5761 tcctgtttta tataataaag agaaaaagat ggaggagctg gttatttatc cagcaaaaat
     5821 gaaggagatg taaaacaaaa tatgggtatc ttcaaattac atagtgatta ccttccccgc
     5881 ggtgaccaac ctgaagcaat cgctgcttta actgaaggtc tgcgtcaaaa taaacgctat
     5941 caagttcttc ttggggttac cggaagtggg aaaactttta ctatagccaa tgtgattgct
     6001 caatttgata gaccagtact ggttctatct cataataaga ctctggcagc acaactttat
     6061 ggagaattta aacaactatt ccctgaaaat gccgtggaat attttatcag ttattacgac
     6121 tattatcaac ctgaagccta tattccgggc aaggatatct acattgaaaa agatgcagat
     6181 attaatgaac agatagataa attgagactt agggcaacaa tgagtttaat ggaaagaaga
     6241 gatgtaatca tcgtagcctc tgtttcctgt atatacggtt taggagttcc agaagattat
     6301 agagaagctt taatacacct caaaacagga atgcaaatgg aacgagatga acttttagaa
     6361 aagctcatta gtgcacatta cagtcgtaat gacattgcct ttgagagagg aactttccga
     6421 gttagaggcg atattgtaga tattttcccg gcatatcaag aacattttat acgagttgaa
     6481 ttctttggag atgagattgt tagaattgaa aagttccacc ctatcaccta tcaaagtctg
     6541 gaagaagtgc cagaatattc agtttatcca gcaatccatt tcttaaccaa tccagaccgt
     6601 ttaaaaagag ctattgcctc catagaatcg gaattagatg aaagagtagc ctattttctt
     6661 tcccagcaga aatatttgga agcggaaaga ttacaaaccc gcactaaatt tgacctggaa
     6721 atgctggcag aattaggcta ttgttctggt atagaaaatt attctcgtca tctgactgga
     6781 gcaaaacctg gagaacctcc taaatgcctt ctggactact ttccagacga tttcattatg
     6841 gttatagatg aatcccatgt gactattccc caaatccaag gtatgtatgc gggagattat
     6901 actcgaaaga aaaatctggt agaatatgga ttccgtctac cttctgcttt tgataataga
     6961 ccgttgcgtt ttgatgaatt tcagaaatat atgcataatg tcatctttgt ctctgccaca
     7021 cctgctgatt atgaacttag tctaaccaat ggagaagtgg ttgaacaggt tattagacca
     7081 acaggtttgc tggatccgga aattgaaatt aaacctatca agaatcaggt agatgacctc
     7141 atcaatcaaa taagggaaag ggtagcaaaa aatcagaaag ttctggtgat gaccctaacc
     7201 aaaagaatga gtgaagacct gagtcaatat ctgcaaaaag cgggaataaa aagtaaatat
     7261 ttgcatagtg agattgattc catagaacga gctcagatta tcagaagcct cagattaggt
     7321 gaatttgatg tgatagttgg tgttaactta ttgcgtgaag gtctggattt acctgaagtt
     7381 tccctggttg ctattttgga tgctgataaa actggatttc ttcgttccgc tcgttccttg
     7441 attcaaatat ctggaagagc agccagaaat gtggatggta aagtggtttt atatgcagat
     7501 gaaatcaccg atgccataaa aaagactttg gaagaaacca atcgtcgtag agctaaacaa
     7561 ctggcatata atcaagaaca cggtataact cctcagagta tcagcaaaac gcttcaacag
     7621 attatggaat ctactgcgat tgctgaaggc tatgatgcta tggaaaagaa agccaaagca
     7681 aaccatttcc gaaaagagga tttctatgat tatctggaac tggataataa agaaaaattg
     7741 ctccagattc taaaaaagga aatgaaacaa gccgcagcaa aactggattt tgaaagagca
     7801 gcagaattaa gagataccat tgcagaactg gaaaagcaag ctaattcttg aaactatgct
     7861 tatctggaaa aataaagtct cactcccaag ggtctatggt ccgaaattat agaggaataa
     7921 gtgctccaag agcccatata atcttccact ttgattacgc gacacacact atctgcttga
     7981 gcaaaagcat caaacagttc attagtgatt aaaatatggt ctaaatgtga tggtggatta
     8041 taatgaggat aggaaacatt atcataggta ggatttaaag ctataggata atcggtaaag
     8101 taatattcat ctggtttatc cagaaaaacc tgaaagacat tagtggaagg aggttcggca
     8161 atctggtcat tcatatctcc aaccactata actctcctat cgggaaaatg ttcagttata
     8221 tactggtcaa gtttttgaca ggcaagcctt cttcgcactt cttcgtccca ggcatcactt
     8281 tcatctatat aatcatctcc cagagcctta agatgattgt tgatacatat aaaatttaag
     8341 cctttataat taaaatcaag gacataaggt ggtctgggaa atgggttact ctcccctata
     8401 aatatgggat agacctcatt aacctgaacc gtttctttat tatataaata ggcaagtcta
     8461 tagctactgg tggcattaga tactaaagct tcatactgcg gaatagcact tgccagttga
     8521 taaaaagcat tttggtccat tacctcctga caggcaataa tatcaacttt aaattgtggt
     8581 attatctgag atatggtctg aactgtatta ttatttaaag gaaaggtttt aagattccaa
     8641 gtgaccactt ccagagtaga ttcagtacca aattcaagta tattcacatt ttccggagga
     8701 gtcagtttat gattagttcc acaactgaac aataggaata tcgttaacat taataacagg
     8761 gttaattttt taatatacct cattttctat ccttaatttt aggtctttta agaaataata
     8821 atattaagca taaagcttgc caaattgtaa aggattattt ttataggtat cttactattt
     8881 tacggaatgt tttttgcata taataaagta tcacttctgg aggatattta aatgaaacgt
     8941 tatttatgga tagctattat gcttgcagta tgctgtttag ccgcagctat gaatgaaaat
     9001 tatataacaa cgactacagg actgcttgct catctggaaa atgttcgtgt agctccagtt
     9061 ctacaacaac ctgaagaacc tgaggaattt cctgagacga ctctcatttc taaaaccttt
     9121 gccctgcctt ataattctat agatttgcag gtgcaaaatc ttcaatggaa tgtttttgat
     9181 agttcaggta attttctcta tcaagaacaa gcaattgaac ccggtatttt aagaattgga
     9241 aatagtttta ctttccggga aatgagaggc tatactatcc tgattgaaac tcagattaat
     9301 gaaggtgaaa ccattcgtac ccttgcttcg gcagattttt ctcttaccgg ttctgaacct
     9361 atagaaatac cagaaaatat ctctccagct tttattgatg catataaagc tttagcggac
     9421 aattttcaga cttcctacct tcgtaattta cctctttcaa gacccaaaat gcttattatc
     9481 agtcatacta acctggcaaa ttaccagact gaatttatca aatggaagaa atcacagggt
     9541 tttgaggtct atgttgcata taaatcagat ataggtaaca cagttaatga tttcaaacaa
     9601 ttcatccaga atcattatta tcaataccat tgtgattttt tgctgctttt gggtgatgtt
     9661 accaatcaag gagcttatag cattcctaca gctttttacc catctcctga atatcaagaa
     9721 aatgatgcgg atgacaatca atacaccttt attgaaggaa atgattattt tcccgaaatg
     9781 ttagtgggtc gtctttcttt taatgatata gcagaattta tggcaatgtc caataaatct
     9841 gttatctatg aaaaaactcc ctatatgtcc aataccaact ggatgaaaag agggttggct
     9901 gtggcaggaa actatgcgga aggtggcctc cgtccaagca ctcccgtaga aatgtctcgt
     9961 tggctaagag ataagatgtt aaattttggt tatacaactg tagatacggt tttctatcct
    10021 ccaacctatc ctggcacctc aaatattata actgccatca atcagggagt gcaatttgtg
    10081 agttatagag gttggggtga tgctaatggg tggcattatc cagcttttca tataccagac
    10141 cttgccacca ctcaaaatgg tcctcgtatg cctgtggtct tttccatagt ttgtaataca
    10201 ggtgactttg ccaatccagt taatcccagt tttggtgaaa aatggatgcg catgggaagt
    10261 gtagctcagc ctaatggatg cgtggcattt gttggtcctt cagatttaca taccaagacc
    10321 agacttaata attccatagc cagtggtgct tttcgtagta tctttgatta tggtgtccgt
    10381 tgttttgcca ccagtgtcct tatgggaaaa atagaactat ataagaattt ccctaatgat
    10441 ataggagata atcaatatgt agctttctac ttccatgttt ataatattca ggctgatccc
    10501 agtttaaaga tgtgggttct ggttcctgat actatatctg aaagcgttat tgaaggtgga
    10561 ttaacctttg ctcaaagtga ttctcatatc cgaatcaatg ctcctcatct taatggtgct
    10621 atggtcacag gaactaaaaa tggaactgat ttcacatata gccaggtaat agatggattt
    10681 gccatcttac ccatagaccc tgaagcaact ggcaatttaa ctatcactat tactaaagaa
    10741 aattatgttc cattaattcg caccttaact tgctctgaac ctgctcaaat tggaattgaa
    10801 gataacagtg tggaaggttc catcctcaat cctaacactg aaatcactgc tcaaatcact
    10861 ttgaaaaact attctgattc cgtttataca gttaattctt tatctattac ctccacagac
    10921 gatgctaccg tagattataa catccaacct tttaccttaa atcctaatga aactcataat
    10981 ataagcttta acattattgg taatccttca atccgtcctc gtaaacctat tacttttagc
    11041 cttagtctac cagaacataa tatacttcac caatttcaaa tacttggagg tggtgcagaa
    11101 tttaccgttc tgaatagtag tggctttata gcaatcggta atatcagtcc agttagtttt
    11161 cagattcaaa atattggcac tgctcctatg acagatgcca gcatagaaat tatttcttta
    11221 actgacgcag cagatttccc caatccaatt cagcatattg gaaacatagc tgtgaatgaa
    11281 cctattatca tcaataccac tatccagaca ggtgctgaaa cctggaatgg aagaaatatt
    11341 cctctgaaga tggtcttaac tgataatggt tatagcacca cagtctatta tgcagttacc
    11401 gtgggaactc cagaacctaa ttctcccacc ggcccagatg aatatggtta ttttgcatat
    11461 gacaattttg atatcggatt tgatgaagct cccacctaca actggataga aacagaccca
    11521 ctacttggag gtcaaggaac catctgggaa gttagggatg atggttcaca tacaatagat
    11581 ttgcctttca ctttccgctt ttatggcaga gactacaatc agttaacaat gtgctccaat
    11641 ggctggattt cttttatatc tactcaaatg catgattttt ataactgtta tattcctgct
    11701 gcccttggac cttacactat ggttgctggc tactgggatg acctgaaagg aatgaaaatt
    11761 gggattgatg aacaaggtaa tggtattttt aataatatgc gtctttcctg gtggtatgat
    11821 actgccaaca atagatttat tatccaatgg aatgatgcct ataatcaata caatatagac
    11881 ctaatggaaa atgcttctct ggaaaaattc cagattattc tttatcctca accagatcgt
    11941 gatggtgata ttgtcattca atatcatact gttgataatc caggtgttac aacaaattat
    12001 tgcacagtgg gaattgaaaa tcatcttcag acggttggat taacctatac tcatgctaat
    12061 acttatcctc ctacagctca tactttacaa cctgggttag ccattaaatt taccaccatt
    12121 cctcctgacc cctatacggg aaataatgat gctttacaga ttccttttca actggctcaa
    12181 aattatccta atccatttaa tccaagaact accatttctt tctccgtagc caaaagtgga
    12241 tatgctaaac tttctgtata caattccaga ggagagctta ttcgcacttt attaaatgat
    12301 aatatttgca gtggtaatta tagtatagat tgggatggga atgatgataa gggtaaatct
    12361 gttagttcag gcttgtatct gtataagctg gaagtaaatg gtatcaatca aattaaaaag
    12421 atgctactgc tgaaatagga tttaagatta tataaccgat acagatagcc atttgcagaa
    12481 tacattaaaa ggaaagttat ggattaatct atttaatcac atatcttatc ctaatagctg
    12541 attaaaacct ataaatgaag acactttttc attgacaaaa taaaactatc taaaataatt
    12601 gaataaagaa taattttata atggagaaat gatgagcgac cagttacagg aattgctcca
    12661 gcgtgtatat gaagaaggcg ttaataaagc caaagcagaa gctgaccaga ttttagagaa
    12721 ggcgaaatct gaagcagctg aaattgtcca gaaagcccaa gcagaagctg atagaataat
    12781 ttctgaagcc agaaaacaag cagaagacct taagaggaac actgaatctg accttcaaat
    12841 ggcagctcag aatactctta atgctgtaaa acaaaagctt actgacatct ttctggatga
    12901 ggctttcaat accaagctcc aggaaagttt ttctgaccct caattcttgc aaaaactgat
    12961 tctggaaaca gtagcagctt ggaaagaatc tgggggaacc attattataa gtcaaaatat
    13021 ggaagataaa ctggataaat tctttttaca atccctttct gaaaaagcat ctaaaggatt
    13081 gaaagtagaa ttttcacctc aaatgaaaag tggcttcgca ctcactccaa gtgatggaag
    13141 ctacaaatta agcttcactg acgaggattt tgccaaccta tttaaatcct atctaagacc
    13201 acgtagcaat aagatacttt ttaaatcata ggttattgat gcacactcaa tattactatt
    13261 ttgtaactgg tttaccggtt ataactattg acgatagtaa gcttcccttt actccagatg
    13321 aatttttgct tgatgcacag gagcatatta ctgcctctga ttttaagctc ttaagatggt
    13381 tgcgtttgcc ggaaaaagtt atagaattag taaatgttat ctacaacaaa gaggataagc
    13441 tccatcccat tgatgaagaa agtctaatat tctataaaga gtatatagac catatgaaag
    13501 aaagggcaag tgaacctacc atacctgtga gaaaagagtt taaaatcttt cctgactttc
    13561 tccataaaat tattactgag ctttatatgg aagaggaatt accaccttat caaaaagtac
    13621 ggcataaatt gctggaagcc acttatgaat tttgcagtaa atcttcggac gactttttga
    13681 aacagtggtt caatttcaat cgtgatatgc aaaacatcat tatcgctatt aatggtagag
    13741 ctcacaatat tgattatgtt ccttatctca ttggagaagg agaactggtg gaaaaactgg
    13801 ctaaggttca caccatggat tttggtttag gaaaggataa cgaacttttt gaaagtatct
    13861 cacgcatctg ggaacagaat aatattctct atcgggaaaa acattacgat atacttcgct
    13921 ggaagtggat tgatgatttt aatttcttca actactttaa tatagaacgt atcctgggct
    13981 ttgatgcaca attgcgtatg ttgaatcgct ggataaagct tgatcctgta aaaggaaaag
    14041 aaatatttga aagcactctt gatacgctaa ccaagagttt ctcatttccc gaacaattta
    14101 aagttaaatc aataagcaaa aaatagaagg ttaagtttat gaccaaaggc accgttaaag
    14161 gaattatcag caacttagtt tatgtggaag tggatggtcc tgtctctcaa aatgagattt
    14221 gttacattga cttccaggga gttaaactaa tggctgaagt aattaaggtg gcaggcaaca
    14281 ttgcctatgc gcaggttttt gaaagtaccc gcgggttaaa acctggagac ttggttgaat
    14341 ttacggataa gatgctacag gtaaaactcg gtcctggtat gttatctaaa aattatgatg
    14401 gtctacagga tgatcttaat aagatggaag gtttatttct aaaacgtgga gaacataccg
    14461 accccttaga tgaagagaga atctggcatt ttactcctat agccaaagtt ggtgacacag
    14521 tattggcagg tgattggctt ggttcagttc cggaaaactg gttggaccac aagataatgg
    14581 ttcccttttt aatggaggga aaatatcagg tgaaatccat tgttccagaa ggggattata
    14641 agataaccga taccattgcc gttgtagtag ataaagaagg gaaagaatat cctttaacaa
    14701 tggtacagta ttggccagta aaacttccta ttaaggcata tcaatccaaa ccacgtcctt
    14761 tcaaaactct ggaaactggt attcgtacta ttgatactct aaatcctata gcggaaggtg
    14821 gaactggttt tacaccaggt cctttcggaa ctggaaaaac tgtattacaa catagtatct
    14881 cgcaaaatgc agaagcagat ttgattgttg tagctgcctg tggagaaaga gctaatgaag
    14941 tggttgaact tttcacggaa tttcctgaat tggatgaccc tcgtacggga agaaaattaa
    15001 ttgagcgcac cattattata tgtaacactt ccaatatgcc agtggcagca agggaagcat
    15061 ctgtctatac tgcgatgtct atttgtgaat actatcgttc tatgggatta aaagttttgc
    15121 ttctggcaga ttccacttct cgttgggctc aagctttgag agaaatgagt aatcgtatgg
    15181 aagaactgcc tggtccggat gcttatccta tggatttacc ggctatcatt tcaaactttt
    15241 attcccgagc aggctatgtc tatttgaata atggtcaaac aggttctatc acttttattg
    15301 gagctgtttc tcctgctggc ggtaatttaa aagaaccggt cacagaatca actaagaaag
    15361 ctgctcgttg tttttattca ctctctcaag atagagcaga tagcaaacgg tatcccgcag
    15421 ttgaccctat tgattcttat tccaaatatc ttgaatatga tgaaattcag gaattcttga
    15481 accagaatat ttcacctggc tgggtggata aggtatataa agcaaaagat attctcctta
    15541 gaggtaagga agctaaagac cagatcaata ttctgggtga cgatggggtt cctctggatt
    15601 atcattctcg tttctggaaa agtgaattga tagatagaat tatcctggtg caggatagct
    15661 ttgaccctat tgataaatct actccaatga aacgtcaagc ctatatgctg aatcttttac
    15721 tcaatatatg tgatactaac tttgattttg aaagctatga ggaattacaa cctttctatt
    15781 ctcgggtaat aaatgtactt ctacagatga actatcaacc ctttgaaggt gataaattca
    15841 aagaatatga agctcaactg gaatctatcc tggcagaaag gagagtgaaa taatggcggg
    15901 aactgcattt caaaaaatat acactaaact aactcaaatc actaaagcta cttgcacggt
    15961 tcaagctaca ggcgtaggaa atgaagagct ggcaaccgta ggtggaaggt tagctcaagt
    16021 agttaaaatt atagataatc aagttacctt gcagattttt gaaggaactg aaggtatcgg
    16081 aactgatgca gaagtaatct ttttcggaaa accaccaacc ataaaagtta gtgaccaatt
    16141 agcaggtaga ttcttcaatg cttatggaga acccatagat ggtggacctg caattgaagg
    16201 tgatgaagta gaaattggtg gaccttccgt taatccagta cgacgtaagg acccttctga
    16261 actaattgcc actggtattg ctggaattga cttaaataat acattggtca caggacagaa
    16321 gatacctttc tttgcagacc ctgatcaacc ttataatcag gtgatggcaa cggtggcttt
    16381 aagaaccaaa gcagataaaa ttattctggg ggggataggg ttatctaatg atgattatct
    16441 ctattataag aattcctttg aaaatgcagg agtgattgat aagattattg ccttcatcaa
    16501 tacaactgat gatccacctg tggaaagatt actgattccg gatatggctt tgactgctgc
    16561 agaatatttt gctatccaga aaaatgaagc cgtgctggtc cttttgacag atatgactct
    16621 ttattgtgat gctctttcca ttgtgagtaa tcgtatggac cagattccgt ccaaggattc
    16681 tatgcctggt tctctttatt ctgatttagc caagatttat gaaaaagcgg tacaatttcc
    16741 tactggcggt tctataacta tcattgctgt gacaactatt tctggtggtg atatcactca
    16801 tgctattcct gacaatacag gatatataac tgagggacag ctttatctga gaagagatac
    16861 ggatataggt aaggttatca tagatccagc aagatctctt tctcgtttga gaaacaaagt
    16921 tatcggaaaa cgcaccagag cagaccatcc tcaagtgatg agtgcttccg ttcgtttaaa
    16981 tgctgatgct atgaatgcca aaacaaagct ggaaaatggt tttgacttga cggattacga
    17041 tcagcgagca ttgaaatttg cccgggatta tgctgagcac attctggcta ttgatgttaa
    17101 tattgatacg gaagaaatgc tggataaaac ctgggcactt ttccgtaagt atttcactcc
    17161 ggaagaagta ggtattaaag acgaatttat gaagctttac tggaataagg aatgaacctc
    17221 aagtttcaat acaataagat tgctcaactc cagctggaga agcagcttaa aatcaggcag
    17281 aatgcacttc ctactctaaa gaataaggaa tcggctctgc gtatggaagt gaagcgtgcc
    17341 agggatactg ctttggattt ggatgggaaa atccgttcca gtataaataa cctggaaccg
    17401 attatgagat tgtgggctga atttaatcct gatcttataa aaattgaaaa tgtagagttc
    17461 cttgcacgta aaattgctgg agtgccagtt cctatcctgg gaaatataca gtttgagatt
    17521 agtgaatatg accttttcag tacaccttcc tggtatctgg atggtattga aatgctaaaa
    17581 gacatagcta ctctgagtat tgaacgagag ttcttctgga gaaaaatgct tatcttagaa
    17641 caggtacgaa aaaagaccac acaaaaggtt aatctctatg aaaaagttca gattcctctt
    17701 tatgaagatg ctatcagaaa gataaagcgt tttctggagg atgaagaaaa cctttccaag
    17761 gcttcgcaaa agatacttaa aaacagaatt agccgggagg ctgctgtatg atagaaaaaa
    17821 tgcggaaata taccttcgtg ctctatcatc aggattatcc taaattcctg gctgatttac
    17881 aaaaactcgg tgtggttcat ctggttcaga atacagaaga aaagactgag acactaatga
    17941 aaaatatgga acagctggaa gaatatgcgg agcagttaag atttttaaag aaattaaaat
    18001 ctaccgcaga aaaaacagcg gttccttatc ctgcacaagt tttactacag aaaatccagc
    18061 aggcaagaga ggaaaaagaa aaactggaaa ggctggctga aactttacga aaacaggtac
    18121 gtgaattaga gccttgggga cattttgatt ataatctggt taagagttta caacaagctg
    18181 gcgttaaagt tgaattttat acctgtctga agaaccattt caaacctcaa tggcaagaag
    18241 aatatgccgt aaagattata aatgaagtta atggaatact ctatttcgtg gtcttatata
    18301 ctgacgaaaa agcagaaatt gaagcagatc cctttagctt ccatcaacat actttacagg
    18361 aactggaatc acaacttaaa gaaactgaac acaggattaa agatatagat gaatacatct
    18421 ataatattgc tcctgctgcc atagaactat ttagtaatga aatagcaaat ctcagtaaga
    18481 cctgcgaatt tgaagaagct gtattacagg gaattgatga agccgagggg aaagttaaaa
    18541 tcctgcaggg ctttattcct caaagattgg aaccggaact ggtcaaattc ctggaaagcg
    18601 aaaatgtgat atattttgct aatgatgcca ctattgaaga taatccacct gttcttttac
    18661 gtaataattg gtttgcccgt ttatttgaac ctatcagtaa aatgtatatg cttccctatt
    18721 ataatgagtt tgaccttact ccattttttg cacctttctt tatgcttttc ttcggttttt
    18781 gtaatgcaga tatggtttat ggtgttctct ttattttaat aggtttaatc ttaaacaaaa
    18841 aaatgaagaa tccattcata aaaagtataa gtgggcttgc cgtattgttt ggcacttctt
    18901 ccctcatatt tggattatta atgggatctg cacttggatt tgatttaaag ggcaccgcat
    18961 taaatcctta tgttctaatc cgagacaata accagatatt taatttttct cttcttttag
    19021 gagctataca aattcttttt ggaacgataa taaatggagt taaaaaagcc ttacaatatg
    19081 gattgaaata ctttcttgca cccttcggaa cctttttatt tttactatca ttaactattt
    19141 taggtgctgg acttttggga gtagataaca gtgctttaac tccttatact aaatatcctt
    19201 tatggatagg attagcttta atgatgttat ttaacaatcc tgcaaaaaat cctttaatca
    19261 atatattaag tggtttatgg ttactttaca atgtggtaac gggtttcttt ggtgatattc
    19321 tatcctatat acgacttttt gctctgggtg tttcatcttc aattctggga atggtgatta
    19381 attccatagg tatgcaattt ttgaatataa aaatacttgg tcccattatt ttctttatct
    19441 ttatggcggc aggtcatact ttgaatatcg ctctgggtgc cttaagtggt tttgtacacc
    19501 ctcttcgtct cacttttgta gaattttata aaaatgcaaa tttccaggga cctggtattc
    19561 cctatcagcc ctttggacac aaaaaaatga ttaaaatata aactggaggt caaatgcctt
    19621 acattctttt acaagcagga accgcagcag ctaatgcttt accaatttct ggtcatacta
    19681 ctgcctatat tttagcttgg ttaggtctct ttttaatggt tgctctatct ggaattggct
    19741 ctgccgtggg aacagttatt ggcggaagtg ctaccattgc agctatgaaa aaaagagagg
    19801 atgtttttgc taattgtatg attttatctg ccctacctgg cacacaaggt ttatatggct
    19861 ttggtgcttt cttcatttta aaaggatttg cgacggcaga tgtaaatatg ataaccgcag
    19921 ccgctatttt tggagcagga ctcatctccg gattagtaaa cctgatctct gcttattttc
    19981 aagcgagagt ggtttcaact ggtattgaaa gcatcggtaa tggacataat gttttctcta
    20041 acaccttaat cttggctgtg tatccagaac tttacgctat catttcattc gctgcctgtt
    20101 tccttatcag cgcaatgtta cccctttgat agaacaacta ttttgccaag tctggacagg
    20161 gaataaattt ccctgtccag attaagtatg ggataaaaat atggattatc gtgattcaca
    20221 tcagacaccc tctggacaac aattccagta ttggcaatat cctcctatgc aaccacatag
    20281 aggagtttta atatttatct ttggattatt aggattattt acctgtgcta tttttggtct
    20341 tcttgcttgg atttttggaa atgaagactt gaaaaaaatg gaacaaggta ttatggaccc
    20401 ttcaggtaga gatctcacca atgcaggaag aattctggga attatctctg tatctattac
    20461 agttctggga ttagttatta ttctggcata tttctttttc gttatgggag ttgcagcttc
    20521 tctttctaat ggagttttcc acttttagca cctgaaacct attccttttt ttactaatgc
    20581 gcttgcgtat tgaaaaactt gatactcgtt ttccttacgg aaaaatccct ttattactat
    20641 ttttctttct gctggcaatc tattttatct tttatctttt tgtcccttat aagattccct
    20701 tatgctggtt taaatcttta acccatataa attgtcctac ctgtggctta tatagagctt
    20761 ttactgcact cctacagggt cacattatta ctgctatatc ctacaatcct ttaatgttat
    20821 ttttgactat cttcctcata atacaattga tttgcacaac tttatttaag attaaaatat
    20881 ccctaaaagc caaggtcaaa gaaaaacatt ggattctatt tttcttcatt attgccttta
    20941 ttgccaactg gttatacatc atttaacctt aatttccagc taatttgttt ataactttaa
    21001 tataataaat taaattattt caacttgttt tactcctatc tcttacttgc ctatttctta
    21061 gcgctttagg caagagatta acgggaaaga gctaaggaag acccaataaa gggcaagaaa
    21121 tgtaataaag tagatggata aaagtaataa aaatcctgta tatgagacta tttgtatttt
    21181 aaactggaaa taaaggctat attataagat tcttattttc tggagaacca gaataaatca
    21241 ttattgtatc caacatttag ataaaagcag ggggaatgtt ctctactgaa agcagcgcta
    21301 aaatggatag gtcctaaata agctctatat ccaatatcag cataaaagct ccattcaagt
    21361 ttctgattta aatcccagaa ttcttcatta acatagttta gactttgcac tcctgtttct
    21421 aaaaaaacat tttttcttgg attcaggacc gatgcaattg tgtaatagca atattgtggt
    21481 gaacttatgg catatcttgg ataagctcta tatccttgag agcctgtaaa gtagaatgga
    21541 tcaagtgagc tttcttcacg agcaccaaaa taggttccga aattataacc tgctcgtaag
    21601 gatattgttc tggaaactgg tgcatagaga tcaacattca ccacggcttt gttatacaat
    21661 ctatcgctaa tctgttccca gggagcaaaa ttaagtttgg aaaaaacacg taaccctgat
    21721 aatgggaaaa ggtcatcatt cagactttca tgatatacct ttacgcctaa accagagatt
    21781 aaataaagag aatctactgg tgcaacagct gaaacatcac gatagagtcg cgttttataa
    21841 ctgtagaaaa aaccttcagc tatagcaaga ttattggcaa agaatcccac ccctggagtt
    21901 attccatact ccagtgattt aacacttgag attttgtaaa aatcttcatc atagagatac
    21961 aatctttttt cactgatata cggaaaaatg cgaaaataag agccccaaaa ttcgccgaag
    22021 tttttaacat aatccaaact caactctgac cttccaccaa gcgtgaaacc tgtaagtaac
    22081 ttagaattct ttagcaccaa attatttaat tccatagctc cactaatatt tagctcttct
    22141 tcggtggtat aactcatatt gaagattata ctgcgtcggt catattcctg cacataaaaa
    22201 ataagtttat atccattttt ataaggttcc agaacaggat ataccgtttt aaagaattga
    22261 gagttccata tttttttagc tgcggtaaca atctcttcag cggtatattc atttcctatt
    22321 tccagcctgg aatattcctt aattttagct ttactaacat acacattgcc tcggcaatct
    22381 tgagctaaga tgcgatattt ttgaggaata ggtatttttn nnnnnnnnnn nnnnnnnnnn
    22441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22501 nnnnnnnnnn nnnnnnnnna ttgcctcggc aatcttgagc taagatgcga tatttttgag
    22561 gaataggtat tttttggggt tctttaaata catagccttc tgctaaaaga gaatcacgaa
    22621 atgctcttat ctcctcaata tggcttcggg cataacgctc tcccgcatca attatctctt
    22681 tagccttatc ataggacata ttagaaaatt cacttaagtc cggttctaag actaaatcac
    22741 ttaaagccag agaactctca tagctctgat acattgcaat ttcaatggtt tgatttagaa
    22801 tataaattaa attatcggga tttttttcag taaggtcagc attaacttta aaagttataa
    22861 ccttatcagc tcctatctca cgcacttgcg gaactggtat attttgtagt attcctgcat
    22921 ctatataacg atgtccatcc agttcaaaag gtgtaaataa actgggtata gaaattgctg
    22981 ctctgatagt ttgcatcaga gaaccactgg tagaaacaat taattcacct gtatcaaggt
    23041 ctaagtaaag tccagcaaaa cttactggta atttagagaa atcacgatag ctggaagcag
    23101 gcatataaac ctgtgccagt gcctgttcca gttttactcc attaattaat cctgtaggat
    23161 aagacggttg accattttcc tttattctaa gctgaaaatt gccataactg ggccaacgtt
    23221 tttgtccgat ataaaggtcc ttgcgtctat aatgttcatc aaataaatcc agaacatccc
    23281 atcttgttgt taacgattct atttctgttg gattatatcc catagaatat agacttccca
    23341 ctaaagctcc aaaacttgtg cctgcaatat attcgggata aataccttct tccgcgagca
    23401 ctttgagcat cccgatatga gcataacctc ttgcaccacc accactaaaa gcataaccga
    23461 gactttttgc ttcagttggt ttgaaagaga aacttaaaag caaaataagt atgattaagt
    23521 agaacttgtt attggacatt ataatttcaa aatgatattt tccttattcc taagaactgt
    23581 gatgggtgct tataataaaa gttattttac caaagacggc attagcatca attttaactg
    23641 taccacgagt ttttgcctca tccatagctg tctttttagg aatgtctata gaaccacaaa
    23701 aagctgtggt agaaaaatca aaattaatat catcaggtag ttctaccaca aaagagccga
    23761 aaactgcatt gatttctaag ggagatgcat ctggttctat attcgttaaa tctaatttct
    23821 gagctgcgaa tatggcatta tattcgcgtt ctgtggcgtt atacaatttg gttggggttt
    23881 ctttgtgctt cgtatttccc cagccaccta taagcaattt gacgcccaga aggataatta
    23941 ttatagctat aaaaatcttg aatatgggga aactaatatt aaaacccttg agtattaaag
    24001 agacaccaat taatattatc aaaattcccc agaacaaatg tccaaaaagg atattcatct
    24061 tcatctcctt ctccctaatt aatttttatt cctgattgat aggctttata taaagcttca
    24121 gtagacacat atatatcttg attaacctta aacattggct tatcagaaac tgttcccagc
    24181 aatttgagag gtatatcata tttgtcacat acagtttgta ctttagtcaa gttttcagca
    24241 ttaaagctaa tcaccactct ttcaatggat tcattgaaat attctacact cttggaaaat
    24301 gtcaaaggaa aatttagttc tgctccggtt aaattttctg gttggaaaca gcattcagct
    24361 aaagcgacca acagacctcc ttccgaaaca ttatgcgcag aagatattat atcttcctca
    24421 atcaaattca ataacacttc tataagcctt ttaataaatt ttaggtcaag ggaaggtatt
    24481 tttccggcaa cagttccaaa gatagtttta agatattgag aagctcccaa ttcactactg
    24541 gttcttccca gaaaagcaat gatatctcca gaatgcttaa accactgggt tgtagctttt
    24601 ttataatttt ccagtaatcc cagcatacca attacgggag ttggataaat tgcgccctct
    24661 ggattttcat tatagaaaga aacatttcca cctgtgactg gagtttctaa ggttatacag
    24721 gcatctttca tacctcgtat agcttcagaa aaagtccaga agacctcggg tttataagga
    24781 tttccaaaat ttagacaatt ggtaatggca ataggtcttc ctccactgca ggcaacattt
    24841 agagctgctt cagcaactgc agctttagct ccttcatagg ggtctaaaaa acaaaagcgg
    24901 gcattacaat ccgtacttaa tgctatggct ttatccgttc ctttgatcac cattacagct
    24961 gcatcacttc caggttcaat cttcgtagct atctgcacca tataatcata ttgagaaaaa
    25021 atagattttt tggaagctat acttggttca cttataagac gatataaagc agaagtataa
    25081 ttttctggct cgggtaatga acttatatcc atacttttct tttcatcaat gtaggaaggc
    25141 tttttagctt cacgagtgta aactggtgct tttcctccca gaaccagata ttcagccgga
    25201 atttctgcaa ggacttgttc attaagcttt actcttagca ttttatcatc tgtaaccttt
    25261 ccaataacca ccgcatcaag gtcccatttt tgaaagacct tttgcacctc ttccagattc
    25321 tctggttcta caatcatcag catccgttct tgtgactcag aaagcataat ctcataagga
    25381 gtcatccctt tttctcggac aggcaccttt tccaattcca attctattcc cactccaccc
    25441 tttcctgcca tttctgaact ggaacaggtt aagccagcag cgcccatatc ctgaatagcg
    25501 accactaaac ccgcttgaat aacttccagg gttgcttcca gaagcagttt ttcataaaaa
    25561 gggtctccca cctgcacagc agttctttga gatgcagatt ggtcactcaa ttcaacagag
    25621 gcaaaagttg ctccatgtat gccatctctc ccagttttag cacccacata caaaaccaga
    25681 ttcccgattc cttttgcaga tgatttggca agatgttcgt gttttgccac tccaactgcc
    25741 ataacattta ccagaggatt accttcataa gcttcatcaa agaacacttc tccacctaca
    25801 gtaggcactc caaaacaatt tccataatca gagataccat gcaccacttc ttttaagaga
    25861 tgcataaccc gaggattttc caggtcaccg aatcttaatg aatccagaac agccactggt
    25921 cttgctccca tagtaaatat atctctaaga atccctccca ctccagtagc agcaccatga
    25981 tagggttcca aagcagaagg atggttatga ctttcaatct taaaacatat agctaagcca
    26041 tcgccaatgt ctactactcc ggcattttct tctcccgctt tagcaagtaa atattttcct
    26101 tctctgggca aagttttaat caagtttata gaatttttat aactggcatg ctcactccac
    26161 atcacactaa agattcctaa ttccagataa gtgggatttc tctttaatat ttcaagtata
    26221 tattgatatt cagtttccag aatgccatga gatgcaatca attctctggt aataggaggt
    26281 tccataattt aactttcatc cttttctata aaattagtgt cttttagctt ttcaattagc
    26341 cgttgaatac ggtcatcaat aatattaaag gtttcacgat aggtgtccag gtctgcacca
    26401 aaagggtcag ctatatctcc tgtttcccct acatattcat tcaaagtaaa tattttatga
    26461 acctgttttg gttcttgcat tctaagaaaa tctctttgcc gttcttccat agttagcacc
    26521 agataacttc cttgtatcat ttcaggagta atctgtttag atatatggtc tcgggcttca
    26581 agaatgcctc tttgcagaag taattgcata gaagctgtag agatagattg tcctcctgga
    26641 agaatacccg ctgaatcagc cgaataaggt aaattaatcc tggaagcata atgattaaat
    26701 agtttctctg ctattggact acgacagata ttagcagtac aaacaaaaag aatagtgggt
    26761 ttctcaaagg atttctttac cgcatagaaa gggatagaac cttctctaat gcattttaat
    26821 tcatcaaatc cggattgatt tttttcttcc cggctgctga tatattctac tatggttgaa
    26881 ggttgagaat tatatggata gtttttattt ccaggtagaa tagcataatc aaaccaatca
    26941 gcatagaaag tagttaagcg ttgtaagtca gattctggtg gtagagaaga aatgtttaca
    27001 ctggtgctta tgataggttc ttccaatata tctataaaga ctctgagtaa ttcatcatca
    27061 ggcaccctaa aggctacttt accatctaca gcaatatgag caaatctttt atcctcacat
    27121 ttaaaaatta ccgttaaatt tccgggccaa tattgttcta aaagcgaaat aagcctatca
    27181 ggaatataga ttccttcttt ttcaaaccat tgaagcgagg aaactaatag gataaagcta
    27241 tgtttatttg atcttccttt tagtttctgg attctttcaa tagcttcata agaagagact
    27301 ttacacccaa tgccaaacat atctccagta taatgaagaa tggttttgtt atcaaaagca
    27361 cctgtagaag caagtttttc cagcttacgg agggatggtc taattaaaat aaccaaactt
    27421 cgtaccttgc atttattcca gataagctaa aggatttact gccttgccgt ctttacgaac
    27481 ttcaaaatgt aaacatggct cacttgcaga accagtcata cctgatttag ctataggttg
    27541 acctttttta acctgtgctc ctctggagac cagaagttca ttattatagg aataaactgt
    27601 aaagaatcca tttttatggt caataattat aagttttccc tgtcctccat aaggacctga
    27661 aaacaccact tctccatcat ctgctgctac cactgtggtc cattcaggga cagcgatatc
    27721 tataccatta ctaataaccg aagttccata agctctggtt tcttcaccat aattacggat
    27781 aatttttcct ctaagaggcc aggcaattga tttaaagctt gttttatagg cttcagagat
    27841 ttctccagaa gctttagatt tagcagttaa ggaagcaatc aatgattcta attgggcagc
    27901 atctttcctc aactgcgcaa tttgattctg taacttctgc tcttctttag ttaattgctt
    27961 cttctgggtt tccagagtct tgactgtagt attaagtttc ttacttagat tggcttttgc
    28021 atataaatca ctctcagcta atttaacttc ttttcctact tcctgttttt ccacagctaa
    28081 gaccacctga acatcgccca attgattcag tttctgtctg gtctgagcag tcataacagt
    28141 taaatagcgt tcctctttgg gtttaacctt agtcgtagaa ctattaatcc ggattcgtaa
    28201 aagtgaattt agctggttgt tttgtagagt cttgagttca cgcacttgct cttctacagt
    28261 ttttaattcg gtgctaactt tggtcagtac ctgttgtttt tgtttaacta cttcttccgt
    28321 tttctttttc tccagctcta ccttttgctt ttcttttaag gtagtttgca gctggacatc
    28381 agtagttttt ttcttttgtg aagtttgagc tgctttcttc tccatttcct gcaggtcttt
    28441 ttgaatcttt tccagtcttt tttgagtatc gcttatttca tctcctatca acagagaaaa
    28501 tataaggaac aggctaagaa agcataatat cttttttctc ataacccttt aaccatattt
    28561 tttgtaagaa acttattgca aagattagct attaatgtca atgcaaaagt tcttttgcaa
    28621 tattatagac accttataaa aaagccgcct aataaaagcg gcttttttat gagtttgaaa
    28681 gtgtttattt atttcttttt ggtaagctgt tttaaagcct ttttctccgt gtgagtgtga
    28741 accaaagtct ctaaaacaag gttactggct ataaagatag aagaataggt tccgataata
    28801 atacccagac tcatagccaa ggcaaaatcg tgtatcacag gtccaccaaa gaaataaagt
    28861 gcaagtgatg ttagcaaagt agttccagaa gtaataaccg ttctacttaa agttttgttc
    28921 attgctctat taaatatcaa aggaactgga tctttacgat aaattttcaa gtcttcccga
    28981 attcggtcat agataacaat agtatcgtta atagaatatc ctaccagagt caataacgca
    29041 gccagaatgg tcatcgtaaa ttctttttga gtgaaggcaa acacccctac caaaattata
    29101 acatcatgaa atatagcaag gatacccata agaccaaagg taaactcata acgaatccag
    29161 atgtagataa tcataaacaa catagcaatt aaaacagcca ttatagcatt atttcgcatt
    29221 tccattccag cttttgcacc gaccacatct atctgacgaa ttacatcagt ttgcaggtcc
    29281 ctgccttgaa catattgagg tagtttagta gccaaaactt ccgtaatctg gtctttaatc
    29341 ttatttacat cctcaccacc tactttaatt tggaaagtag cattggaagg gtctcctata
    29401 aattgaattt ctgcctccct gaaccctgct tcattaatga ctttgcgtaa attatcaata
    29461 ctcaaaggtt gaacattagg gtctttaggt tgcatattga taatgagtga gactccacca
    29521 gtaaaatccg tactccagtt tatgcccatg acgattatgc ctataattga ggctaccacc
    29581 agcactgcag aaattacgaa gaatatataa cgcttttcaa cgaagggata attagcattt
    29641 tgaaatagtc tcattttttc actcctcaga tactcatcgt atttttgcca ggtttaatca
    29701 ttgttttttc cataatataa cgagcaaaaa cgatggcaca gaacattgaa cctacaatac
    29761 caatagatag ggtaaccgca aaacccctaa ctggtcctgt tccaaattga tacagtacga
    29821 gagcagcaat aagagtggtt atattggagt ccaatatggt gatagttgct cttttaaagc
    29881 ctgcatctaa agctgatctt ggcgatcttc cggcatccag ttcttctctt attcgctcaa
    29941 atattaaaac attcgcatct actgccatac caatggttaa aattatacca gcaataccag
    30001 gcatagttaa ggtgccgcca aaaatggtca ttgcagcaag aataaatccc acattgaaga
    30061 tgagtacaaa atcggctatt aatccagctc ctttataata cagagccata aaaatgatta
    30121 ctgccaaaat gccaattaat ccggctctaa atccactttt tatgctatct tcacccagag
    30181 tggcagaaac ttgagtgcta tatttgggtt ctatgggagc agataagctt tcattattca
    30241 aaaggatggc aagagatttg gcttcctgct cagtaaattg tcccgttatc atagccttac
    30301 catctggaat acggtcctgt atcaccggag cagaatagac agtgttatcc agtacaattg
    30361 ccagtctttt acctatatta tctccagtac aattagcaaa ttttcttgca cctgtacgag
    30421 tgaaagttaa agaaatatat ggtttgttag caatctgagg attgggacta tcagatggtc
    30481 caatttccat actggctcta tccacatccg ttccctttaa ttgaggtgta gattccagca
    30541 catacagcac cactggttct ctacttgctt tctttttctc atcaacactt tctaacacta
    30601 aatcataacc tttgggtttt aaattgacaa aggtgctatc ttgaagcagg gaacgaacaa
    30661 tatctaaatc ttcataacga acagcaaaat ccctttctgt ggtggttatc aaagatttaa
    30721 agataccctc accagtttta acagaatctg ctcctgcctt agctaaagct ttatcctctt
    30781 cagccagttt agccagaaca gggaatttat ccagattctc ctgtattatg gcatcaactt
    30841 gattgataaa tttttccgca atatccggtt ctgctaccag tttaaattcc agcacagcag
    30901 atttcataat caagttttct gctgctatag gatctttaac tccaggtaat tgaaccagga
    30961 tatcgctttc ccccatcttt tgaatggtgg gttcagctat gccaaattgg tcaatacgat
    31021 tcctgattat cctgatatta ttttccaccg cagccgcttt atctgcttct ggcaaatctt
    31081 tagtcaccac ggttagaaca atctgagtgc caccacgaag gtcaagacct aattttagct
    31141 tctgttttat aggccaattc acatcctctg gcacaaataa cggagacagg aagatcaaag
    31201 tacccaaaac gaatattaat atggatacag gagttaacca ggaattcttt ctcatatttt
    31261 taagctccct aaatctatta ttagctataa aaaagcctta ttatatgcca cttctttaca
    31321 ttacttcatt tcgtcaagat ttaatttagc agtataacgg gttagaaata aattaaaatt
    31381 cttcacttat tcatatattg catgtataga aagaacgctc aaattttttc cctggatttc
    31441 ataaatttcc ttctcatttt tctaagtata ggcaaaagag agttctaata acacctcctc
    31501 ccttctgaac acacattccc aagaggggaa tttgggcttt aaaatacagc tattttttat
    31561 taaaagtcta ataagctatg ctgtttgctt tggggatgtg aaatttttta gatatataag
    31621 taaaaaagac tggactaaaa tgtgccctta gaataatgaa cttaataaag acattatatc
    31681 ataaagttgg ttattggaat ataatttgct ttaattaaaa ttaaactaaa tagaaataaa
    31741 gttggaaaat atgaaaaaac taatcttagt tcttaccctt ttggctggca ttatttatct
    31801 atcagccaat gtaatttata cttataactt agcagctcca gagattaaaa cggaagggaa
    31861 ttatataaag gttaacttat cgggagcaca aagctatggt aatgctggag caccagattt
    31921 accctggatt ggcagtaaac ttcttttacc tatgggagaa gaagcagttg acattcagat
    31981 aaatttatct ggtgctaaag atatagtttt agacaaaaaa atagctccta tccaacctcc
    32041 cataaaacta tcggagattg caattgcaca aaggatagac cctgatccag caatttacga
    32101 taggtcagtt gcttatccag aacaaaaaca taatggaagt aatactcaat attttgctgg
    32161 tcatcctata aactttaccg ctatctgtcc ttttgattat aatccggttg aaaataagct
    32221 aaccttttac catcagataa caatcactat tataaccagt ccttcttcaa aagccgttga
    32281 agcaaagaca cttttgaaac aggatgtgga tactgcagat agacttaaaa aaataattga
    32341 taattcccaa gcaattccgc gatatgaaga acgcttaata ggttacgatt atttaattat
    32401 ttatgaccag gcaaaacaga gtcagtggtt acctttcaaa actctttatg aagatcgtgg
    32461 tttaaatgtt tatatgaaac ctattcaaga gattgtagca caaaatgctg gaattgatac
    32521 tcaggaaaaa atccgtaact atatcattag cttttattcc cagcagccct taaaatatgt
    32581 tctacttgca ggcgatacag atgttattcc tcatcgtggt ttttatgtaa atatgggaag
    32641 cggaggtgaa gttgatgcag atattccagc tgatatgtat tatagctgtc ttgatggaaa
    32701 ttggaataca gataatgata attattgggg tgaacctatg gaatcagatt tagttccaga
    32761 actatctatt ggacggtttt gctataattc agatgatgag atagccaatt ttatcaataa
    32821 gacaagtatg tatctcaata atccagtaat tcctgagata aaatctgcat tttatgtagg
    32881 agaatatctt tgggaaggtc ctacctgggg tggtgattat atggatgaaa tgataggtgg
    32941 aagtagtgct aatggctata ccactgtggg aattccggtt aattggaata taactacgct
    33001 ttatgataga acttacggat cttctaattc ctggggtgct tcccaaattc gtcccttact
    33061 cagtcaagga ccaaatttcg ttaatcacct cggtcattct aatactacct atgctatgcg
    33121 actggctgta aatcaagtca ctccaactac aataactaat aatggagtaa atcataattt
    33181 ttctattata tttactcaag gttgttatgc gggagccttt gacaatagag aaacagatgt
    33241 tggctcctac acagttgatt ctatcacaga gaaatttacc agcatatcca cagcagcagt
    33301 tgcaatgata gcacattctc gttatggctg gggtgtagta ggttcaacag atggagcttc
    33361 ccagcatatt caccgtcaat ttatagatgc tatttttgga gagggaattt atcaattggg
    33421 caaagctctt gatgacagta aaattgataa tatcccctgg ataacaaaca ctcctgtaat
    33481 gtattgggta acttatgaaa ccaatgttat tggagatcca gctttgatgg cttggactga
    33541 tactcctcaa cagattattg ctgaactccc ctcttactgg attgttggat taaactatta
    33601 tcagattcaa actaatgcgc ctaatgctct cttccgtata aagaatggag aaaatttaat
    33661 ttgtgaaacc acggcagatg cttctgggat gataacggtt acatttttag gtaatctcac
    33721 tcccggtact atagatgtat atattattgc acctaacttt ttaccattcc atcaacaagt
    33781 agtcgtagaa gccagtaata tgccctatat agttccaacc acaattgcat taatagatga
    33841 tgatggttta tatcatactg gagaagaaat aaatataaat gcaactatta aaaatgtagg
    33901 tttgatggat caaacgggac agggaacaat cactctatta agtaattcca gcaatgtctt
    33961 agtgcttaat ggtagtgctc agttcaatcc actggctgtt gctgattcca cggtaattgc
    34021 agggattttc agaatcagga ttcaaggtta ttttcctgac catcagatag caaacctcaa
    34081 tttcctcact acctttgatg attattcggt tacgactcca tttataatta ccttaaatac
    34141 acctgaactt aatatagatt catataactt tgtaaatgac tgtcctttaa taatgcctgg
    34201 agaccaacct tatcttaata tgttggttag caatagtggt tcaggaaacg cttatacacc
    34261 aatgttaatc ctatttagtg ataatcccaa tataattctt tctgaaagtg aagtcttact
    34321 acctcctgtc agtgctggta attctgtatt ttgtgaaaat atattcagtg tttatatctc
    34381 tgatgccatc agtgcaggaa ccgcaataaa tataggttat atgctttctg ctgaaaatgg
    34441 aaatactata gaaggtaatt ttgttttata tgtaggcaat cagcattata cttatgaaaa
    34501 tgattttacg ggatgggatg tgttagcacc gcttcctaac tttatcaatc aatggcaccg
    34561 ggaaaattat aggaatcata ccactaatgg aagctggtct atgaaatttg gtggtattgg
    34621 aaatcttaac tatgctaata gtgcttatgg tgtcctggaa agtccagaat tacctttagt
    34681 tctaaatgct caactccatt tctttcactg gatggatgct gaaaatcata ctaccaatcc
    34741 acagtatgct tgggatggcg gattagttca aatgtcttta aatggtggag aatggactca
    34801 aattacacct ataggtggtt atccttataa gatatatagc aatgtagctt caccctttcc
    34861 cgcagaaaca agagtctatt ctggaacctt tgactggact gaagctgtat ttgacctctc
    34921 tggatatact ggaacagcta aattcagatt tatatttggt tccgatggag cagtaactaa
    34981 agaaggttgg tatatagatg atttgtatat ggaaaacgaa gtccccaata gtgacttggt
    35041 tgaaactatc cattttgaat tatatgaaaa ctatcccaat cccttcaatc ccactactac
    35101 tatatcgttt aacctgccta aaaatgctcc cgttaatctt gagatattca atttgaaagg
    35161 acaaaaagtt cgcactctaa taaatagtga attaaaagct gggaaacata atattgtctg
    35221 gaatggtctg gataataatg gaaaacccgt agcaagcgga gtttatttct atcgtttaag
    35281 caatggagaa caaatccttt ctcgtaaaat gatgttgatg aaataatgaa ggttaccaaa
    35341 gatacccgtg tccttgtttt aactggtgca ggcattagtg ctgaatcagg tcttaagact
    35401 ttccgtgatg cgggaggttt gtgggaaaat catagagttg aagatgtggc aacccctgaa
    35461 gcattcagaa gagcaccagt aatggtctgg aacttctata aagaacgctg gcaacaagct
    35521 cttcaggcta aacctaaccc tggtcatctt gctcttgcta aacttgaaaa ttggcttcaa
    35581 gataactttc atctcataac tcagaatgtg gatggacttc ataccgcagc aggaaataaa
    35641 cgagttattg agatgcatgg ttcgttacat acctgttttt gcacccgctg ccatactcgt
    35701 tttcctatga aagatatcaa tttagaggaa gatattcctt tatgccctaa atgtaaagga
    35761 atgctgagac cggatgttgt ttggtttggt gaggttcctt atcatctgga taagattgat
    35821 gcactattac ataattgtga tgtttttatg gtagtcggaa ccagtggtgt ggtctatcct
    35881 gcggcaggat ttgtgatgac agctcgttat tttggagcac ataccatagc agtaaatctt
    35941 aacccgatgg aaaatagtga gtttatagat gaattttatc agggcaaggc aggagagatt
    36001 ttgcctcgcc tgattgatga gtggattaat tcagctgata aataaaagag ggttgaatga
    36061 ccctcttttt aatgtattat tcttgttccc gttttccctg agaaggcggg aacaattttt
    36121 tcaatggaag tgatgagcac ctcttgccca cctcgttcaa gaaaatcaat agctgctttt
    36181 atttttggac ccatactacc tgcgggaaat tgattttcat tataatatcg ctgggcttct
    36241 ttaatggtta aataatccag gtctttctgg tctggtttgc caaaattgat agcaactttc
    36301 tctactcccg tcaaaatgac aaataaattt gcctctatat ttaaagccag aagagaactg
    36361 gcaaaatctt tgtcaattac cgcatctaca ccttcataag tgccatcttc ttctatatag
    36421 actggaattc cacctccacc tacagcaatt actatcttac cttcatgcac catttcttta
    36481 atagtctcag ctggaattat tctctgcgga agcggagaag gtaccacgcg acgataacct
    36541 ttacctgaat cttctctaag tttccaacct aatttctgct ccagttcttt agcttcttct
    36601 tgagtataat aggtgcttcc tacaaacttg gtaggattaa gcatactggg gtcattttta
    36661 tctacaataa cctgagaaat gatggtgata acatctctct caattcccga taagagaagt
    36721 ctattctgca aactctgttc tatcatataa cccatagtgc cttctgtggc tgcattaagt
    36781 acacctaaag gaagtggtgg aattccttcc ttttctccca gttcctgttg acgaagtaaa
    36841 tttcccactt gaggtccatt gccgtgggtt atagcaagtc tataaccttg acgaatgagt
    36901 tctaccacac cacctaaaga agcacgagta tttgcaaact gttcagtaat agttcccttt
    36961 tctcctgctt taatgatggc atttcctcct aaggcaagta aggcaatttt atccataata
    37021 tcctcattta gaattaaggg gagcagattt cagattctgc tcccatttaa ttaattatat
    37081 cttaagcttt atctaaatat taataatcct ggagaacatc ttccagattg ggtctaccta
    37141 tctcttgcaa ttcataggtt acctgacagg ctggatgttt aatattaatt atacgtctca
    37201 aatcaatagg ggtggcgata acaacgctat cgcattctgc atcattaatg gtcttctcca
    37261 ggtctttaat ttgacggtca ctataaccca ttgcaggtag aagaatgcct ataccaggat
    37321 acttttcata agtttcggca atttcaccca ctgcccattc tcttggatca accaaatctt
    37381 cacatccata cttatcagca gcaacaactc ctgctccata agtcatttca ccatgagtga
    37441 gagttggacc atcttcaacc acgagaactc tcttaccagt aatcaattca ggatggtcta
    37501 ccattaaagg agaagctgct tctataatct tagcccgggg attgattgca cgaacatttg
    37561 ctcttacttc attaatatcg tccagatctg cactatcaat cttattgatt atcactacat
    37621 ctgccctata tagattggtc tcaccaggat gataggaaat ttcatcacca ggacgatgtg
    37681 gatcaactac tactatatta agctgtttat cagaataata gaaaggaaaa tcattatttc
    37741 cgccgtccca gacaattata tctgcttctt tttctgcttc acgcagaata gcttcataat
    37801 ctactccagc ataaatgatg ctattcatct tgatgtgagg ttcatattct tctcgttctt
    37861 caatagtgca atgatggaaa tccagatctt ccaactttgc aaaacgctgc actttttgtt
    37921 tcaccaaatc accataaggc ataggatgac gaatagatac cacatttcta cctttcgctt
    37981 ttaaagcttt gacgatggca cgagtggtct gagattttcc gcaaccagta cgaacagcac
    38041 acaccgttat taagggtttg ctggtcttaa tcattgtatt tttcgtcccc atcagcataa
    38101 aatctgctcc acaagcattt accagagaag ccttatgcat cacaacttca tgagcttggt
    38161 cactataggc aaacaccact aaatcaacat taagattctt aatgagttcg ggtaattctg
    38221 tttcagaata aatctttatc ccatcaggat acaatcttcc tgcaagctct gcaggatatt
    38281 tcttatcgtc aattccagga atctgggtag cagtaaaagc tacaacttca tattcctcat
    38341 tatcccggaa aaagacatta aaattgtgaa agtctcttcc ggctgcaccc attatcacta
    38401 ctctgcgttt cattactttt ctccttttga taattttatt tcttttatat aaagcaacct
    38461 tttattatag ctttgtgcgt caagataaat ttctgattta aaggattact gcgtgaaagg
    38521 aaggaatggt ttcttaaatg caatcttaaa agagattaat tattcggtag taaaataaca
    38581 ccttcataac tttataaatt aaatatggga gtgtacagat aatcgggtcc aaaagaagac
    38641 tcaattattt gaataactcc taatttaaag atatcttcta atccttttaa ataaatcaac
    38701 ccagttcatt ttaaaaaaat ttaaaataaa aaataaaaaa taacttgaca aataatattc
    38761 aaagtttaat taagaattta gaaaaaatga ttgaggtgag tgagaaaaat caccattata
    38821 taaataagat gttagaataa aggatatata aaggggaata aaatgaaact taattttatt
    38881 aattattatt ttaagataga attagaaagg aggtataaac aagaaaaaaa aaattaggct
    38941 aatggcacaa aggtgttacc gtaaaaagaa gggattgaca cctaaattta aaaatgattt
    39001 aaaagatcaa aatatcgtag ttgagacaac agctttagct gttaatacta tttgatgtat
    39061 taaatttcga aattaattaa acaggtattg agtgatatgt tagaaagaaa gagaatttat
    39121 atttacttaa gctcaaacct gccaatacca cattaaaaaa gagcttagaa aatgaaagga
    39181 gtctattttc ttaactcttg caaccttata accaaaaaaa attcaaggag atattaaaat
    39241 gtttacccaa gaaactaagc attccttaaa ttatttttta aggagttttc agctcgttat
    39301 aataatcact ttgtgcttat ttatgtcttt atctttttta tatggtatag gatcagaaag
    39361 taagactaat tcttttgatt ctggtgattt tccaaaatat attgattata tactgtatcc
    39421 agatagtgga ccctctataa ataatgtatt atatgaaata gaaagcagat ttaacaacaa
    39481 tgaaaaggac cattttactg tattattgag caatggaatc tacaatcaat ctttttattt
    39541 tgaattatgt agttttagtt ctgcattctc tgatttatat attgaaataa ggggttcaag
    39601 taaaaataat gtgcaaatac aaggtggcaa ttatattaat acctatggtg ctgctaactt
    39661 acattttaag ttaactaatc tttccattaa taatggcttt agaggtatag aagcttatac
    39721 tgattacgag aataactata ttactttttt ggaaatagat aactgcagta ttcatgacaa
    39781 tatcagccaa tcatttggaa tagatgtgtg tatgtatggc gcaggtatat atagcaatgg
    39841 tcctactgtg attactaatt gtgatattta taataataaa ggcaagaact ggatacaccc
    39901 agcttatctc aattattccc gaggtggtgg tattgctata gtaaataata ctatttataa
    39961 ttctgaaata acggactgta atatatataa taatgaagct aatgccggag gtggtatata
    40021 tgttagtggg acagcaccga taacgattgc tcgcaataga atccatggga atacccgaaa
    40081 actctataat tatgtaacag caggatttgg agaaggtgta tactgttacg attgtcataa
    40141 acttactttc aaggataata tagtatatga acaaatcccg gcttcatatg aaaaagatcc
    40201 cagagaaccc atcccaattg aagcagcggt agtaattgaa tcctgtggtg tttcaccatt
    40261 aatagcgagt ataaagatag aaaacaacag tataatggat aatgatgaat gtcatggtct
    40321 ctgggttatg atgcccgaag gtgggacagt tattaggaat aatctatcct gcagaaattt
    40381 aagtggtatc tatttatcca attatagcag tgggtatctt actttagaat ataatgatgc
    40441 ctatgataat ggatacttaa atcagggtga aatactaaat tacttcattc aatatccaac
    40501 agaaattgtt tcttttaata attatgaatt agaccctcag atagacacat cttatatgcc
    40561 aatctgggat tcatctacca taagctggat tattgatgca ggagatccag cccttactga
    40621 ttctgatggg acccgcagtg atattggagc aataagagca ggtgagcatt attatgagga
    40681 atatagtatg ccagtaaatg gaggtatcaa gtggatgagt tttcctgttc ttaatactgt
    40741 cactgaaggt tatacgacca attccaattt cttcgctcct attttatacc aaaatatact
    40801 tgattgggta gactggaaaa aagaagataa tcagcaatta agaatgcaaa tcattgatgg
    40861 ttatgttcat aatggagatg gtaccgtcag cagtccagta ggatataagg taaaggtcaa
    40921 agatggagtg agtaatcaga taaagattat taccaccggt tataaggaat cagacctaaa
    40981 gcagattcat ctttataaat atctctcagg aactaacata gaaaatgaga actggatagg
    41041 ttattttcag aaggagagtg catatgctct ggatgctttt gctcctgtac tggataaaat
    41101 tttaatgata aaggcacaat attggagtat ggcaagagat ccaataactg gtatttggaa
    41161 agtaacgcag gaagatttga ctattaatta tggagatatg gtcattgtga cggtgagtga
    41221 ggattgtagt tttatctgga ataataaaca accagtagat cccaagtaca gggagttacc
    41281 ggagcatttt gagtatatag agaaggagga ttatactcct ctttatctgg atttaacttc
    41341 tttggaagaa ttaccttcgg agattggagt ttatgtgaat aatgagtgta aaggtgcagt
    41401 aaaagtggca ggagattcta ctgatatctg tgtatatata ggtgcaaatg aaacccttaa
    41461 tcctgataac tgtgagctta tattatatta tgatacaaag ggtatacaac cggtacgtat
    41521 ggtttatcag cctgaggcag gtgaactgat tcaactatcc aatggaggga taagattcta
    41581 cacgctgaaa cttaagggta gtcccaattt gaaacctgtg attccggtga ccagattact
    41641 tcagaattat cccaatcctt tcaatccgag tactaccatt gtatatgaaa tcggggaaga
    41701 gggacccatc tgtatggaga tatataatat aaggggacag aaggtaaaga ctctggtaga
    41761 tggttttaaa gtagcaggaa tgcatagagt ggtatgggat ggtagagatg ataatggcag
    41821 aagagtagca agcggtatct atcaatatcg tttaacaacc agagatggga gcattaccaa
    41881 agagatgctt ttattaaaat aggcagataa tatttctcag gggagaaatt atctcccctg
    41941 agaattttag gcaaaggagc ttaagtatga gaaagagagt atggttagtc ttgtgtatat
    42001 cggttatgat agttaccttt tctgctacga ctttacgggt tgctctggat ggtagtcaag
    42061 tttaccaatc aattcaatct gcgatagagg atgcggtagc cggggatacg gtattagtat
    42121 atccaggcag atattatgag aatgttgaat ttatggggaa atctatcagt gtttgcagtc
    42181 tggaagctgc cacgggagtt agcacttata taagtactac cataatagat ggaggaatgc
    42241 aggatccttg tatagcgatt cgtgctgatg agctggaagc tacggttcgg ggatttaccc
    42301 ttaccaatgg tctgggtttc ttagaatggc agactgataa ctatagatgt ggaggtggaa
    42361 tatatattta cagagatttt caatcccctt attccaagca ggtgaatgtt atcaattgtg
    42421 atatcacaga aaatagagct gtatggggcg ctgggatata ttgtattgag agttcattat
    42481 ttttgagcgg agtaaatatc catcataatt atgccagttt gacagcagga ggtataaatt
    42541 tttatgggac aatacataag tctccaaata tagtgtttga tccggttaat ctctgttcag
    42601 tctatgctaa ttatggaagg aatcctgtgg atattttaat tacagatact attggtgata
    42661 cctatattaa tctggattta gcttcggtct tacatcctga tgtattttat atagacagga
    42721 taaaaaattt atctatttat tatccctata acaatatctt gaatattcaa cgcggttacc
    42781 ggacagagat aaatagtgac ttctatgtaa gtccgacggg agataatgcc aatacagggt
    42841 tgagtccaga tcaggcactg aagagcatag cggaagcagt gcaaaggata gcttcggata
    42901 gtctggaggc aaagactgtc tatgtattac cggggaccta taatgaagat gagagttttt
    42961 atcttttccc catcccttta aaatcttatg tcaatattat aggtgctggt tccgataaga
    43021 ctattttaca ttccgatata gcttctccta ccgggaatac ccttttttgg ggaggacaaa
    43081 atcttcagca tattactatt catggtttta ctaccagtgc ggaaataaat cagaactgtc
    43141 gtcctatgat tagcttctat atcagtcatc ttacaatatc tgatatgata ttagaagatt
    43201 ttcctattta taattataga gatgctgcta tattcttata tgaacctact tctttgtcta
    43261 ttgattctct ggtaatcaga aacataactg ttcctgaaca tgctgtctat atccataata
    43321 ctcttcaaga ttgtagcata actaattcca cctttgagaa catccattct acttatacct
    43381 cacctgatac tccaggagat gatagctggg gtccgactgt tgttgaaatt attaccttag
    43441 atactttgac ggtagataat tgtattttcc ggaatatctc tgtacagaat aatcaagcga
    43501 cgctgtatat aggtaataat gtttattttc ctggcaatgt aggcaattta aaggtcacta
    43561 actgtctttt tgataatata aggactaatt acattagtgg ggtatgtttt atcacaaata
    43621 tctatggcag ttttaaagta tctaactgta ccttctacaa taattttggg actctgggag
    43681 cagttatggc taaaggtaag atagatatgc gaaacaatat cttttataat cctgatgctc
    43741 agtatgaact ttatatgggt gtcccagatc tggatttata cagcgtaacc cgactggatt
    43801 ttgattacaa tgatatccgc aacggataca atgggattag aaatagcagt cctaccaatg
    43861 acctttacta tagagagcat aaccttagca gtgaaccctt atttgccagc ctcacttatg
    43921 gagattctct gtatctgcgt ctgggggagg gttcaccctg tatcaatgct gggacaccag
    43981 atacaaccgg tctcggactt ttaccttatg atctggcagg aaactggaga atatgggatg
    44041 gatgtattga tatgggatgt tatgagtatg gttctgagcc ttatgtagga gtagaggatc
    44101 cggtgctacc ttcaattcct ggatataata tcttagccta tcccaatcct ttcaatgccc
    44161 ttaccaatct gaaggttaaa cttccctctg actcaaataa tacattcaat aaggttaatt
    44221 ctgccagtat agaggtctat aatatcaggg gcgagaaagt gaagactatc aatcttgatg
    44281 ttagtaaagg ggctgaacaa tggacttact gggatggtag agaccagcat agtaagctct
    44341 gtccttcagg aatctatctt cttaatctca agttaaatgg taaatcagtc ctgaccagaa
    44401 aggtcactct aattagataa gcattataag cctaaataaa ttctcagaaa agagaacatc
    44461 tttcctgaag atatttgaat gtacgcgaca aaggagctta attatgagaa agagagtatg
    44521 gttagtcttg tgtatatcgg ttatgatagt taccttttct gctacgactt tacgggttgc
    44581 tctggatggt agtcaagttt accaatcaat tcaatctgcg atagaggatg cggtagccgg
    44641 ggatacggta ttagtatatc caggcagata ttatgagaat gttgaattta tggggaaatc
    44701 tatcagtgtt tgcagtctgg aagctgccac gggagttagc acttatataa gtactaccat
    44761 aatagatgga ggaatgcagg atccttgtat agcgattcgt gctgatgagc tggaagctac
    44821 ggttcgggga tttaccctta ccaatggtct gggtttctta gaatggcaga ctgataacta
    44881 tagatgtgga ggtggaatat atatttacag agattttcaa tccccttatt ccaagcaggt
    44941 gaatgttatc aattgtgata tcacagaaaa tagagctgta tggggcgctg ggatatattg
    45001 tattgagagt tcattatttt tgagcggagt aaatatccat cataattatg ccagtttgac
    45061 agcaggaggt ataaattttt atgggacaat acataagtct ccaaatatag tgtttgatcc
    45121 ggttaatctc tgttcagtct atgctaatta tggaaggaat cctgtggata ttttaattac
    45181 agatactatt ggtgatacct atattaatct ggatttagct tcggtcttac atcctgatgt
    45241 attttatata gacaggataa aaaatttatc tatttattat ccctataaca atatcttgaa
    45301 tattcaacgc ggttaccgga cagagataaa tagtgacttc tatgtaagtc cgacgggaga
    45361 taatgccaat acagggttga gtccagatca ggcactgaag agcatagcgg aagcagtgca
    45421 aaggatagct tcggatagtc tggaggcaaa gactgtctat gtattaccgg ggacctataa
    45481 tgaagatgag agtttttatc ttttccccat ccctttaaaa tcttatgtca atattatagg
    45541 tgctggttcc gataagacta ttttacattc cgatatagct tctcctaccg ggaataccct
    45601 tttttgggga ggacaaaatc ttcagcatat tactattcat ggttttacta ccagtgcgga
    45661 aataaatcag aactgtcgtc ctatgattag cttctatatc agtcatctta caatatctga
    45721 tatgatatta gaagattttc ctatttataa ttatagagat gctgctatat tcttatatga
    45781 acctacttct ttgtctattg attctctggt aatcagaaac ataactgttc ctgaacatgc
    45841 tgtctatatc cataatactc ttcaagattg tagcataact aattccacct ttgagaacat
    45901 ccattctact tatacctcac ctgatactcc aggagatgat agctggggtc cgactgttgt
    45961 tgaaattatt accttagata ctttgacggt agataattgt attttccgga atatctctgt
    46021 acagaataat caagcgacgc tgtatatagg taataatgtt tattttcctg gcaatgtagg
    46081 caatttaaag gtcactaact gtctttttga taatataagg actaattaca ttagtggggt
    46141 atgttttatc acaaatatct atggcagttt taaagtatct aactgtacct tctacaataa
    46201 ttttgggact ctgggagcag ttatggctaa aggtaagata gatatgcgaa acaatatctt
    46261 ttataatcct gatgctcagt atgaacttta tatgggtgtc ccagatctgg atttatacag
    46321 cgtaacccga ctggattttg attacaatga tatccgcaac ggatacaatg ggattagaaa
    46381 tagcagtcct accaatgacc tttactatag agagcataac cttagcagtg aacccttatt
    46441 tgccagcctc acttatggag attctctgta tctgcgtctg ggggagggtt caccctgtat
    46501 caatgctggg acaccagata caaccggtct cggactttta ccttatgatc tggcaggaaa
    46561 ctggagaata tgggatggat gtattgatat gggatgttat gagtatggtt ctgagcctta
    46621 tgtaggagta gaggatccgg tgctaccttc aattcctgga tataatatct tagcctatcc
    46681 caatcctttc aatgccctta ccaatctgaa ggttaaactt ccctctgact caaataatac
    46741 attcaataag gttaattctg ccagtataga ggtctataat atcaggggcg agaaagtgaa
    46801 gactatcaat cttgatgtta gtaaaggggc tgaacaatgg acttactggg atggtagaga
    46861 ccagcatagt aagctctgtc cttcaggaat ctatcttctt aatctcaagt taaatggtaa
    46921 atcagtcctg accagaaagg tcactctaat tagataagca ttataagcct aaataaattc
    46981 tcagaaaaga gaacatcttt cctgaagata tttgaatgta cgcgacaaag gagcttaatt
    47041 atgagaaaga gagtatggtt agtcttgtgt atattggtta tgatagttac cttttctatc
    47101 catacttttt cataattttt ataatttact catcctaatg aaaatagtct atatctcaaa
    47161 ataggtgaga aatataataa ttgaattatc ttttaaaatt ctgatatttt aggattatta
    47221 taaaacagcc tttttaactg aatcttctga accacatctc caaggcatat atattataga
    47281 gcatttctgc ctgagaatta ttgcctttaa gatatttttg ccagttatcc attatgagag
    47341 gaaggttcca gattccacga ttacggaagg tgctggaggt tatgagagat tcataggaag
    47401 ttttcattgg acctcttagc cagagagaaa taaagggaga gccaaagaca gatttttctt
    47461 tgcggttaaa gatggcagtg ggaacgagag gttttatagc ttggcgatgg atatatttac
    47521 cctgagcatt atgaatttta tattcggaag gaagagagaa ggcaagctct accaaattat
    47581 ggtccagaaa aggaacacgg ctttctatgg aagcagacat agacatacgg tcttcccagt
    47641 gcagtaaagt tggtaaagag gtggtataga cagcattata gagaaagttg gaaagtttat
    47701 cgcaggttgg tttagaaaag ttatctgagg taagagtgcg ggtattattc agtttaatgg
    47761 gatgttgaga aaagaatttt gaaacacttt tactggaaaa atcggaaatc tgagccagaa
    47821 taggttcgga aagagatttt ttacattgtt gaaggaaatc ctgattgaat ggctcaaagc
    47881 gaaggaagcg gaactcaaag ttatagagtt gaggttcaga taaaaagata gagagtagag
    47941 cttttgccag tccagaaaga cctttaatac agccatattt cttgagccag gcaagagctt
    48001 ctttcaaggc tttatggaat ttagtctgaa gaattaaatc tgccagaaag cgatagaaag
    48061 aatgacgata acctgctgtg agttcatcac ttccttgtcc atccagaaga actttgatac
    48121 cattttcctg agcaagtttc ataactgcat attgtgcagc aaaaccagag ccaagaggaa
    48181 ggtcattgaa ccaagtaaca tcattaaacc aagccatagc atcttctgct tgaggtgaag
    48241 ttagatggga aatacagcca ctggaatgga ctacctctgc aatccatttt cgttcatcca
    48301 gtggaggata ttccggaaaa taggcagaga aggtctgtaa tggtctatcc gttaaccgtg
    48361 aagcagtgca gacaatagcg gaagaatcca gacctccact gagacaagaa cctacttcca
    48421 catcggaacg catctgcaat tgaaccgctt tttcaaaagt ttctctataa atttctattg
    48481 cttccggcaa agaaagagaa ctttttccaa agcttgcagg attcaattta taatactctt
    48541 ttagcgtgat tccgtgctga tcaacgataa ggttatagcc aggttggaga gctttgattt
    48601 cttgccagaa agtttcctct ccatagacct caagagagtt aatcttcatt gagcgccaga
    48661 tcatcggcag attaaggctt ttatccactg gagtgagaag tagctgtttg atttccgaag
    48721 cgaaatacaa aaaatctcta ttcctacaat aatagaaagg tttaacgcca aagcggtcac
    48781 gggagcagaa aagcaggctt ttatttgcgt cccagagagc aaaagcccag ataccattga
    48841 aatgcaggac acaattttcc ccccagaaat cataggcttt taaaataact tccgtatcag
    48901 attcagaatt aaaagaatat ccctgagcga tgagttcttg tctaagctct atatagttat
    48961 aaatttcccc attaaaaact attagcagat taagctgagg gtcaagcata ggttgatgtc
    49021 ctttcatgct aaggtcaagg atagctaaac gacgaaatcc caatccaaga gaagctctgg
    49081 cagaaagatt taaggatgga tagagctgac gaatttcttc ttttgaacca tcaccgctga
    49141 aaggaagaat cttatgacta tttttttcta tcaaacaaaa tccttcatca tccggaccgc
    49201 gatgttcaat cagagcattc atagccttca ataggctgat atctataacc ttttgggtat
    49261 caaaagaata tataccactt ataccacaca tctaaaactc cactttgcaa gctcgttaaa
    49321 gaggataaaa agtcaaggga tttctcccta taagtgccat aaatcaacgg tcatttgaag
    49381 ttctattgac caattttagc agtctacctt ctcctgcagg aagttctatc tcaatgtttc
    49441 cattatgata gggataaaat ttatcctcgt agggatcata gaaagcataa ttttgatagg
    49501 gattttttct cccactatct ttaaaggtca gaaggagttt ttggggagga gcctcaggaa
    49561 agtaaatatc aaattcttca gggggaacaa attgtggttc cgtgattatg ccagggagga
    49621 aaaaattggc tctacgatta actaccatta accacagatt ttcctcttta tcaaggtagg
    49681 tggcacattg aatatagccc tcatattcac ctattccctg cttctgaacc tgaatatatt
    49741 gaatgggatt atctttctct gctttcttaa attttttgcg agaagtgcca atgcactctg
    49801 aattttgcca gttcaaatcc ttgattataa ctccgtaagc ttttatgcgg ggattggata
    49861 aactaaccgc tctccaggtt ataggatcgg gcacaagcaa aggataatca ggcattccga
    49921 cacgagaaaa agcaatagac ctttgtccat aacctttaat atcctgataa gacctcagac
    49981 aatagtggaa gatgccgtca ggtttaaaac atagaggcag atagagcagc actttttgag
    50041 ctgcagtagt gggttgtatc caatctaccc attgttcttg cccttgatat aaagtccatt
    50101 ttcccagaac ctggacgata ggataaaact tccttccttc ttttttatct ctatattcca
    50161 tacaatcttg atatacactc aaaagttttt ggtccagtat atactgaata aattttcctt
    50221 tctctccttc agtggcattc catattaaat ctggctttaa aggatagata tcaggagcta
    50281 taatttgcgg ttgggcaatt ttgctaaagg catctatatg gtcgtaatat attccctgtt
    50341 ctttatttaa ggtaatattg gtaacttgaa aatcatagac cgcagtgaaa acagggatat
    50401 cttcctgaga agcaatctct tgcatcaatt taaaggaatc aaattgtccc tgatagggtt
    50461 catcaaaggt gtaaaaacct ttaacattgc cttcaccttg agagattaca ttatacatcc
    50521 gttggagaat tttatcttgc caaaaatttt tatcgtgact gattttatga tgtagctcat
    50581 cttctatttc cacataatcc agttctacat cacaatttcc ataccaatag actctgggat
    50641 tgaggttaac taagcggagg aactctctgg aatcagaggt ggcaggatta tggtcaatat
    50701 cagcagtcaa gagattagcg tctataaggt ctttataagg tatctttagt tccagctcta
    50761 aaaattcatt acctttgcga tttaatagat agttatgcac tgtaaaaata gtctcacttc
    50821 tttgctgatt atctatccag tgattcagaa tcttcatttg gctggaaaat cctgttccat
    50881 aaagttcaaa accggttaca taaaatcgca agagaggtgt attagaacta attcctggtt
    50941 gaagattgct tattctaaaa cggaatttca cccaaatata gtcatctgga tagtctggtg
    51001 gattagtctg atataggata aattcactgc caaaacgga
//
LOCUS       DAORZF010000007        47737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_620_length_47737_cov_339.214337, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000007 DAORZF010000000
VERSION     DAORZF010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 47737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47737
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_620_length_47737_cov_339.214337"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..991)
                     /locus_tag="PK015_01855"
     CDS             complement(59..991)
                     /locus_tag="PK015_01855"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HOL33253.1"
                     /translation="MGMKNLSRFIPLVLLLMLVFTLCSCDKFFSPKENINRGKSEETE
                     LASSESSINSIDSLTDLNSGDNGSKTLANVPLTPYPYTKLNSSELPDVAIIVDDFGYN
                     GKLLERFAQELPAEVAFAVLPNLPYTRKAGEIASQYGHPVLIHIPMEGTGVQEKQCIT
                     NNSSADEITALLDDFYSQLPMAVGANNHQGSSVTENREIMTIILDWLNKHNLFFVDSA
                     TSKNSLAQSLAYSRGYPALKRDIFLDVPDDTEQTLANKISSLNKYQGRKEPIIIITHC
                     HNEKKLSNLQSFIREIRSQGLHLTSIINAKNIAA"
     gene            complement(982..1467)
                     /locus_tag="PK015_01860"
     CDS             complement(982..1467)
                     /locus_tag="PK015_01860"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HOL33254.1"
                     /translation="MRKGILFIVVFCLALGFNGCKSKPEEKSFDSIKGTEFRKDATLT
                     ITAPTGEVKAIYEIEIAATEAACTQGLMYRESMAENRGMLFDPKGPSETPFWMKNTYI
                     PLDIIFIDKDKKIMHIAENNTPFSEEMIAPNGIYRYVLELNAGQAEKHNLKIGDLVQW
                     E"
     gene            complement(1471..4380)
                     /locus_tag="PK015_01865"
     CDS             complement(1471..4380)
                     /locus_tag="PK015_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOL33255.1"
                     /translation="MKKILVIALFLVSSAIAFSYPVHITSWDIDSDVKILNSLQISVD
                     NVNRNTGTIIVYVRDDNEFDKLLNNGFKAEKLPDLARQNALELQKIDSLEHTKDTYYT
                     ITQYQQFMIDTANQYSNICSLIQAGTSVQGRPIYFLKITDNPDIEEAEPEFKYVSSIH
                     GDEVVGYDMCIRLIQLLTTQYGIDPRITNLVNSTEIYICPMMNPDGYVLGQRYNANGV
                     DLNRNFPMPTGIQHPDGNQWAPETIAIMDFSNAHHFVLSANFHGGALVANYPWDYTYT
                     LTPDNDVFIQAALTYTSHNSSMYNSTEFPHGITNGAAWYVITGSMQDWNYGYTDDMDI
                     TMEIGENKWPPASQLPSFWALNQESMLSYMEFVHKGIHGLVTSTTGQPLNAVITVQGN
                     DKAIHTDPDVGDYHRLLLPGNYTLTASAYGYIPQTAQITVPASGSTEYNFILSPAATV
                     DLMGQIRDVEGYGIPNLTVTLNTVPPRSSTADTNGSFMFMQIPEGYYHITFSNANTTL
                     YETDFLLTTENNNCVFNFIEPLVLFYDPCESLSNWTASGPWGLVTYQGESVITDSPNG
                     NYGNNVTRILQLNNPISLENILNPILSFKTIYALENGYDFVYVQASNNASNWINLGSL
                     TGTQSSWTTFSYSLSQFVGQNVYIRFKLTSDYGITADGIYLDDIKISGIDANLIVYGD
                     VNGDRMISMNDVQLLLDYIVGYDSLPEIDPFPWENNRIAAADVDGNDILNSVDAYFMA
                     QYIQNPQFRFYVQGAQLDNLPEVTLTMDETGENNTVEAVFQILPENNLKCLDWGLVPS
                     DSLSNLTIEENLGLIYRSAFNPEQGKYAYINLGSPITEGFTISYQTTAVSIDFFYSVN
                     GRDSSFTILSDTDANDPLNPVIPFNLSQNYPNPFNPITYITFSLPVRAKAFLGIYNLK
                     GQLVKTLINGEMNSGIHRVQWNGLDEAGKPVSSGVYFYTLQSGNKTITHKMILLK"
     gene            complement(4448..4546)
                     /gene="ffs"
                     /locus_tag="PK015_01870"
     ncRNA           complement(4448..4546)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PK015_01870"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     assembly_gap    4750..4849
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5190..11288)
                     /locus_tag="PK015_01875"
     CDS             complement(5190..11288)
                     /locus_tag="PK015_01875"
                     /inference="COORDINATES: protein motif:HMM:NF025707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33256.1"
                     /translation="MLKRFCLTFIILTIIILAFAKEQPQPRIYLEGYHPLIYNKVDVY
                     KLYKGLNYIKSQPYWIREFEPDTKEQINYPEEKVIIHTKIDDFDLVPPRILSFDTYFA
                     NLQEKAFYKSMLSLYLTQAQQTTVTKTGMIKEFSIDLPSIAVPKAVQKVLGSSAGRLN
                     IDGTEKISMEVGSTKRKNVAIYETNTASQLDIKMRQDTNLRLTGTIGEKIGVNLKYNS
                     NQDQQFFDPDNINIKYTGTEDELFQTIEGGNIALSLPGSRYISYSAASQGLFGITSKL
                     KYKNLDLTFIASTEEGQKNTQHYVGTSQADSTIFRSRDYAPRTMYYLANPYDVYELYT
                     QADVSSNVPAGWVNNAVKTDPTGAWIIKNPNLLPANGTVRLFIDDGNANNNVAAAIGD
                     TIFFSPTNYYVPYYDELIEGTDFITDYSAGIIRINSNVDRRATIAVKYTQKDGIPVPA
                     NSEAQDGILHVKVLRRRNQEYDPHDPNNVWHYQMRNVYDMHKNNIKSDGFTLDIYTLN
                     VDLTRNYLVPDSLATSYITTYTDYLRMDSNGDGLINGDDNTVNLSTGLITIPFIQPFD
                     PLGDGIIYTDEGESINYLDISFYMSVKGKIGREYVDLSQGGILKGSVSVKVNGIKQKE
                     NVDYLVDYDFGRITFLTSAGKDPDAKIEIDYEYRSTFDISSKTLTGVRADWNLTDWAK
                     LGGTFIYRSENVADRRPRIGNENIEMYMTDIDGSLTVKPTFITRMLNALPLINTTADS
                     RITLSGEIAYTIPNIYGDPHGKKNEAYIDDMEAIIDSYPLGVTIGSWSMASKPWNTSL
                     AKGRTIWYNPKNIHREQVEDPATLTDREKKESVTALAIKVFPANLGIEGSEVWSWGGV
                     MKYLGNQLDFSQKKYIELMVKIDRKANDPVAHPILRIDLGDINEDFYTEYGGLNVLNT
                     EDKNHDGVLTLEEDTGLDGIPDGHPGDDPNDHADNSIDQFGDYPFINGTEGNKVLDTE
                     DLNNDGVLNTLDRYFSYSISLTDSQYLESENEYGWRLYRIPLTDPLVYEIVNNAPSGA
                     APTLKKISYGRIVVETDVPVKVLLYDINVVGNKWEDFYVRDINGHIVPSNIINTYNIS
                     YLSGIVNNQRNSAHYTSPPGTYYIEERRESSESALSLDIQNLQPGQQVLLRQRLFEPY
                     SLLSYNKIKFWVYPELSSSSPINPDSLDIIFRIGADSLNFYQVRERIHVVNYLPKMNR
                     SYWTQFTYELQDFTKLKEMFPDVNSGEYREDNKIYSFKGTPTLTNVRDIYLGLYVPDD
                     LNIPQPYNGTIYFNDMRVAEPFEDVGVAKRLSLNSAFADVITLDIDYEDKSENFNTII
                     QRGRTNTFTSTKTLNIMNKFFLNKLFPNAWGLDIPLSLSRNYNLGIPRFLANSDLLYD
                     NILDPVEKDRQRTENLVYAADFGFSQRTAPKSPFLFYTVYRTSISGRIEKSYRYTPTT
                     VDTTFNWRGTLNYNLGIPSDKVSFILFKNYRIGWFPSTFNNSFTLSSSEPQSYNWEKR
                     EGVYDWYPRTQTVPTKTFTSDNNISWGLTSDISLSARINTERDLKQKIYWQDINIGKM
                     TRYVQDLGLNYNPNYLRNYINLTASASARYSDSQRKYFQNTDEGQITMYQSDGNSNRT
                     IRTNLTLLNSSLLSSWAVNLSSSHSRKEGMKKQDEIKDSDKEPKSPSDEDKKKKEEEW
                     KKQQEETEKYGDNGKGYENGFKEEIYFGKEDIPTEEPGFKDNNDLTQPPKDKDKDKGK
                     EETYFPVTLLNLFSKVKNITMTYQNSYIQSYSRKDDRPPFLFQLGLPHTTPAGYLDSV
                     SDDNTISIGSGITFSRNFDSIISYSYSLQKNRANASNQTEVITFPDLTLSFINFENWF
                     GMNKFLSGARLNTGLQYTTRATGDLDWIEPKQESVSLALNPLVGFTGTFFRKVNTNIS
                     YSITSGTNTTDMDTYDIVKTNLTHAVNGNISYSFTQGKGFTVPFTHKKIHINNELVSS
                     LSVLYEKNYDKTKGRESSQVDRDYTHFAISPSATYQFNPDIRGGLTGTWDQSSDNKRD
                     TGVRTFSLGIWIELNL"
     gene            complement(11293..12081)
                     /locus_tag="PK015_01880"
     CDS             complement(11293..12081)
                     /locus_tag="PK015_01880"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOL33257.1"
                     /translation="MEKYSETTTLVVDIGNTNITCGIYQQDEMVRFARFYSSAHRTAD
                     EYFSLLIPLLNTESLNNINSIVIGSVVPELMRIWMHLFQKYFSAEVWEINALSPLGIS
                     YKVDDPSFIGADLIANAFGALKKYDSDCIIIDLGTATTIQFITKEGSFEGTAIAPGLK
                     VSADALFEKAALLSQIEIVPPSVLLGTNTRDALLSGIVNGHAYMLECYIQQIKTHYAD
                     CQKIVTILTGGIADLVYPLVPSVDIVDKTITLDGFYLAYKTIRH"
     gene            complement(12210..13094)
                     /gene="era"
                     /locus_tag="PK015_01885"
     CDS             complement(12210..13094)
                     /gene="era"
                     /locus_tag="PK015_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOL33258.1"
                     /translation="MDNPQFRSGFVTILGKPNTGKSTLMNRLIGEKLSITSPKPQTTR
                     YNIKGILNRDDCQIVFIDTPGYLKPRYKLQEKMQDLWSESYKDVDLILFMSDIISFPT
                     DYDLEVLELLKKIPIPQIAVFNKIDLQKDYSREFFINQLPESCSEAFFISATRGDGVP
                     ELMEAMLQFIPYHPPYYSEEQLSDLPMRFFAQEFIREAIFNYFSQEIPYSTAVLVEKF
                     KELDDKIHIDAVIWLERESQKPIIIGKNGAGLAKIRDYAEQQLTNFMGVPVMVHLWVK
                     FKPKWRKKPSDLKELGFK"
     gene            complement(13155..14396)
                     /gene="rocD"
                     /locus_tag="PK015_01890"
     CDS             complement(13155..14396)
                     /gene="rocD"
                     /locus_tag="PK015_01890"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HOL33259.1"
                     /translation="MSNNLVYGTISSNEAMELEEKYGAHNYHPLPVVLAKGEGVFVWD
                     PEGNRYYDFLSAYSAVNQGHCHPKIINALIEQAKTLTLTSRAFYNNKLGEFEKYITEY
                     FGYDMVLPMNSGAEGVETAFKLARKWAYEVKGVEKNSALVIFAENNFHGRTMTMVSAS
                     TDPDCYEGYGPYLPGILKIPYNDVDALKNCLEKYGENVAAFIIEPIQGEAGVIVPEDG
                     YLKACYELCKVHNVLFVADEIQTGLGRTGKLLACDYEEVRPDILVLGKALGGGVIPVS
                     AVLADRDIMLVIKPGQHGSTFGGYPLACAVAKASLEVVQEENLAQRSFVLGEKFRTAL
                     RDIQHPMLKLVRGKGLLNAIVVEPKNSYEAWDICLKLKEKGVLCKPTHRHIIRLAPPL
                     VITEDQLMEVTEIIKNVFNEI"
     gene            complement(14585..15907)
                     /locus_tag="PK015_01895"
     CDS             complement(14585..15907)
                     /locus_tag="PK015_01895"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOL33260.1"
                     /translation="MNIAMVGSGYVGLVSGTCFAEMGNRVICVDNDPRKIEMLRNNIV
                     PIYEPGLQELVEHNAKDGRLSFTTDIAEAVQSSQIIFIAVGTPPDEDGSADLQYVLAV
                     ARDIARYMQDYKIIVDKSTVPVGTADLVRKTVKEILQDRGVDIDFDVVSNPEFLKEGA
                     AIEDFMRPDRIIIGADNPRAAEVLRELYLSFNKVNERIIIMSTRSAEMTKYAANAMLA
                     TKISFINELSRLCEATGADISEVRIGIGSDSRIGYKFIYPGVGYGGSCFPKDVKALIH
                     MFQENNLNPQLLEAVEEINKEQKMVLVDKVKSHFGDNLQGLTFAIWGLSFKPKTDDMR
                     EAPSITIINALVDAGANIQAYDPAAMNEAKRIFGSQPKIHFATDEYDALKDADAMLLL
                     TEWHQFRYPDFEKIKATLRQPIIFDGRNQYEPKMMKELGFKYYSIGRV"
     gene            complement(15904..16851)
                     /locus_tag="PK015_01900"
     CDS             complement(15904..16851)
                     /locus_tag="PK015_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOL33261.1"
                     /translation="MKRKILITGGAGFIGSNVADAYLADGYEVVVVDDLSSGSLENLD
                     SRVKFYQLNICSPALEEIFAREKPDVVNHHAAQISVPYSIENPINDAEINLKGFLNVL
                     QCSVKYGVRKVILISSGGAIYGEAEEYPASENCPLNPLSIYAIHKLTSEYYVRFYQYQ
                     YNLTYTILRYANVYGPRQVAQSEAGVVSIFIEKLLHNQAPMLYAYPDEPEGMIRDYVY
                     VKDVVRANVAALDKGDNDVFNIGTGQETTTSFLYRTIAWQLGTDLRPIPGPPRKGDLR
                     RSLLDCSKAFKELGWSPLYTLDAGIRETIEFFKNKESLK"
     gene            complement(16848..17423)
                     /locus_tag="PK015_01905"
     CDS             complement(16848..17423)
                     /locus_tag="PK015_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HOL33262.1"
                     /translation="MSTHKPVEIEELFGKIELAPENHFWSVIHTKPRCEKKLAEYALK
                     HNISYYLPLTESSRIYQRRKVTFTKPIFPGYLFTIINFYTKRELLYSGYIVSYINVPS
                     QKELVDELRNIWLTRNRRIEMREAMWLSEGLMVEVINGPLKGVSGIVESHKKLEEVHL
                     QVNILHQAVSVKVDPRDIKIIGDYEIVEEEE"
     gene            complement(17429..18832)
                     /gene="ahcY"
                     /locus_tag="PK015_01910"
     CDS             complement(17429..18832)
                     /gene="ahcY"
                     /locus_tag="PK015_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOL33263.1"
                     /translation="MEYKVADISLADFGRREIILAEAEMPGLMAIRSKYSETKPLQGA
                     RITGSLHMTVQTAVLIETLIELGAQVRWASCNIFSTQDHAAAAIAARGIPVFAWKGET
                     LEDYWQCTWNALTWQDGGPDLIVDDGGDATLMVHEGYRLEEQYYATGKLPEIDANSYE
                     MRIVQELLISGLQSDPEKWHRIVKRLKGVSEETTTGVNRLYQKLAHQELLFPAINVND
                     SVTKSKFDNLYGCRESLADGIKRGTDIMIAGKVVMVCGYGDVGKGCAKSMRGFGARVV
                     VSEIDPICALQAAMEGFEVNTVDYMADYADIFVTATGNCDVIRVDHILRMKEGAIVCN
                     IGHFDNEIQVQKLYELEGIKRENIKPQVDKIYLPNGKYIILLSEGRLVNLGNATGHPS
                     FVMSCSFTNQVLAQIDLWQNNYEIGVYTLPKILDEEVARLHLDKLGVVLTHLTRQQAE
                     YINVPISGPYKPEHYRY"
     gene            complement(18880..21099)
                     /gene="pnp"
                     /locus_tag="PK015_01915"
     CDS             complement(18880..21099)
                     /gene="pnp"
                     /locus_tag="PK015_01915"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOL33264.1"
                     /translation="MNNFNIIKRELEFCGRRLYIETGRMAKQANGSVFIRYGDTSILV
                     TATMGKEPSEEQDFFPLTVDYVEKMYASGKIPGGFFKREAKPTTDATLAARLIDRSIR
                     PLFPDGFRNPVHVVVNVLSYDGENEPSEIGVFGASLALMISDIPFHGPIAGVTVGYIE
                     NQFIINPSQNELNNLSQLNLAVAGSATSVVMIESAAKELSEEVMLDAVYAGHEEIKKL
                     CALQNDFVAEAGKEKIEVVLQSIPEEIMAKVEADYGSRVAEAAMLFGKQERQDAFDTT
                     EAEMLEKYQAEAENEETYAQNEKWYKAAYEELIRRYVRDSILNKHHRVDGRSLDDIRE
                     VTCEIDVLPIVHGSALFTRGETQSLGTVTLGGSSDEQIVDGLETEYKKRFYLHYNFPP
                     FSVGEVGKMGAPGRRELGHGNLAERALIAVMPDKEIFPYTVRVVTDTLESNGSSSQAS
                     ICSGCLALMAAGVPIKAPVAGIANGLIMEGENYVVLTDIMGLEDHLGDMDFKCAGTRD
                     GITAMQMDIKIEGITKDIMKIALDKAHNARLKILDIMQECIAEPRKELAPTAPRIEAF
                     QVPVDKIGEIIGPNGKMIKSIIEACQVEINIEDDGWVHILSPNQESIRKAKELIKSII
                     REPEIGEEFEGQVTRIEPYGIFVKILGGTKEGMVHISQMHTSRIGSPEDMVKLGDIVH
                     VKALGMEKGKLSLTMKGVEGNPEPNHNSTKQDRPNRRDDRKGSYHPSGHNNPNYRKR"
     gene            complement(21299..21568)
                     /gene="rpsO"
                     /locus_tag="PK015_01920"
     CDS             complement(21299..21568)
                     /gene="rpsO"
                     /locus_tag="PK015_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOL33265.1"
                     /translation="MTLTPEAKKAIMAEFRLHESDTGSPEVQVALLTRRINDITEHLK
                     QHPKDFSTRRGLLKLIGQRRRLLDYLKKKDIARYRELIKALNLRK"
     gene            complement(21794..22021)
                     /locus_tag="PK015_01925"
                     /pseudo
     CDS             complement(21794..22021)
                     /locus_tag="PK015_01925"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     assembly_gap    22304..22403
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            22450..22809
                     /locus_tag="PK015_01930"
     CDS             22450..22809
                     /locus_tag="PK015_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOL33266.1"
                     /translation="MLAKCKCKNISKAKLTEIIDGLPEENIIDQLCEFFRVFGDSTRL
                     RILYFLSCDEFCVADLSNLVGMQQSAVSHQLQTLRLHRLVKCRKEGTTSYYSLDDSHV
                     QEIFGIALKHINEVLVK"
     gene            22806..24878
                     /locus_tag="PK015_01935"
     CDS             22806..24878
                     /locus_tag="PK015_01935"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOL33267.1"
                     /translation="MTTKEYDIENLHCAECGSRIEAGIEKLPEVESANLDYQNKRLSI
                     RYHKAVDDPLARLYQVAASIDPGVVFTLHPVSMINKLSSLFWIFLVIGVGLLIATEFV
                     PSTYQPWLGILGWFLVGYRVLAQALKSLTQKKVFTEHFLMSLATIGALILGEYLEAGA
                     VMIFYEFGEWLEDKALEHSRQMVKNKLNIKTEIAHLRTEDDIQDKPLSTISTGDVIVI
                     YPGERVPLDGTIIKGESTVDTSSLTGESEPLYVGEGTEIYAGFLNNSGMLELQVKSVA
                     SESTISRILKLIENAGAAKSPQEKFITRFARIYTPAVVAAAFLVFLIPTLLGAEAAIW
                     FKRSLVFLIVSCPCALVISIPLTFFISIGIAAKKGIIVKGSGYLDILRQVRTIVFDKT
                     GTLTTGEMQIDKVYVNGDINPEVLLEVLWLCEYNSSHPFSRAIKKAYQGSFDSKQVKA
                     YSEFPGKGLILIYGQDRLVAGSDDFFANMGFLDLINPANQSVVHLAKNGFYLGCITFS
                     DEVKPGMKEAIEKLKKQGIKKTVMLSGDRAPKAEKVAKELGIDEFYAELLPEEKLAKL
                     EDIINKNEGKTAFVGDGLNDAPALARADVGIAMGGIGNQASIESADIVLLNDRPHQLV
                     EAFTISRQTGNMVIQNIVLALGIKALIMLLGILGIAGLWEAVLGDVGVTILVIFNSLR
                     LLRYKADT"
     gene            24934..26838
                     /gene="thrS"
                     /locus_tag="PK015_01940"
     CDS             24934..26838
                     /gene="thrS"
                     /locus_tag="PK015_01940"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOL33268.1"
                     /translation="MQIKITLPDGSEKIYDHPVTAIQVAKDLSPLLAEEAICAEVDGK
                     LVDLDYLIDHNARLTIHTFKTREGKEVYWHSTAHLMAQAVKQLFPQVKVAIGPAIEQG
                     FYYDFDKDEPFTDDELQQIEERMIMLSKENLPYTRQELSKEEAKKIFSELGESYKLEL
                     LEDIPDTDTISVYRQGDFIDLCRGPHLPHTGKIKFIKLLKSSGAYWHGDEKNKMLQRI
                     YGISFPTAKELDDYLEFLKQAALRDHRKLGKDLDLFSINEEVGPGLVLWHPNGAMIRH
                     LIESYWKEQHLLNGYKFVYTPHIGKASLWETSGHLGFYKENMYSAMDIEGQDYYIKPM
                     NCPFHIAIYNTNLHSYRELPIRLAELGTVYRYERSGVLHGLMRVRGFTQDDAHIICTP
                     EQLNEEVEKLIVFALDMLKHFGFKEFLIYLSTRPEECVGDFTDWCTATESLRQSLDKL
                     GLEYEVDEGGGAFYGPKIDIKIRDALGRAWQCTTIQFDFNEPTRFDMTYIGADNAPHR
                     PFMIHRAILGSIERFFATLLEYHSGNLPLWLSPIQLIILPITDNQLDYANKLKEQFLQ
                     KGIRCEVDARNEKIGYKIREAETKKIPYMCIVGAKELENNILTLRRHLLGDLGAMTFD
                     EALNRFREEL"
     gene            26871..27839
                     /locus_tag="PK015_01945"
     CDS             26871..27839
                     /locus_tag="PK015_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HOL33269.1"
                     /translation="MSTLDLIREELNHEAEAIKKVAEQLNPEEVDKAFNLLRNCKGKV
                     VLTGIGKSGIIARKISATLASTGTTSIFLHAAEGVHGDLGMLQPDDVVIAVSNSGNTQ
                     ELLSIIPYIKYIGIPLIAITGNPHSPLAETADAVLDCSIPPEYEALGMVPTSSTTAEL
                     ALGDALAILLLKDKGFSLEDFARFHPGGNIGRKLLIKVDELMHTGDELPLIGEEAAMS
                     DAILEMTSKKLGCVAVVDKDKHLTGIITDGDLRRQIQQKQDNLLQHKVKECMTPNPKC
                     GYPDQLAAEALKLMEDHKITMLPIVDNTNSLVGMLHMHDLIEAGIL"
     gene            complement(27842..29677)
                     /gene="glmS"
                     /locus_tag="PK015_01950"
     CDS             complement(27842..29677)
                     /gene="glmS"
                     /locus_tag="PK015_01950"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOL33270.1"
                     /translation="MSNIVGYIGQRNALPIVIEALKRLEYLGCDSAGCAIIHNNKLQI
                     YKKQGKIVDLERSLPEPNQCSGNVAIAHTRWATHGEPSEVNAHPQIDCHGKIAIVHNG
                     IIENYNPLKKQLIELGHTFRSETDTEVIAHLIEQYRKSEPTLEDAIRAALSKVEGTYG
                     LVILCEDEPDKILAIRKGSPLIVGIGENEHFISSDVSAIVIHTKRVFYLQDDELCILQ
                     KDHFDITNGLREKIEPEVSTVDWDISAIDKGPYKHFMLKEIFEQPTTVENAFRGRIDE
                     TSGNARLGGLKLTPKDISKIEQIHLVGCGTSYHAALIGKYMIEDLARIPVQVEYASEY
                     RYRNPIIQEHTLIFVISQSGETADTLAALREAQSKGAKVFGITNVVGSSVARESNGGS
                     YIHAGSEIGVASTKAFTSQVTILFLLAILLGRQRNLSSLQGMEYIRELQTIPSKIDSI
                     LKQSEYIRQIAETIKDARNAMYLGRGINYPVALEGALKLKEISYIHAEGYAAAEMKHG
                     PIALVDEEMPVVAIATNDPLYDKIHNNLEEVHSRKARLITITNEGNKDLEKISESVIY
                     LPSTLQDLQPLLTVIPLQLLAYYVADLKGVDVDQPRNLAKSVTVE"
     gene            complement(29674..>30612)
                     /locus_tag="PK015_01955"
     CDS             complement(29674..>30612)
                     /locus_tag="PK015_01955"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HOL33271.1"
                     /translation="AVLIGNYQLSHLLLTIGSTLVLDVIAIWITVKLFNTESVLFRTQ
                     EEGGKIKIKNKRTFFSPFYGIVYFCLALVALYYLGGKWQTADLVNGLIQSELILILFP
                     VLIVLRLGKYKPAEILRLNPPKAKELAIIPFIAISASTIVSIIAQVINHIFPFPQEYI
                     EAMSRIFTQDISFWELFIVIALIPGICEEILFRGFLMRFFEQKHFWYPIITTAILFAV
                     FHLDPFRFLPVLLLGILLGYLAMRTGSIVNSMFFHIINNTLAVVITTFGEQNWMKIFI
                     QDGENLKYWVILPAIFIFIISMKVFNKITVSKEVQL"
     assembly_gap    30613..30712
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<30713..31545)
                     /locus_tag="PK015_01960"
     CDS             complement(<30713..31545)
                     /locus_tag="PK015_01960"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOL33272.1"
                     /translation="MNFKKAIVIYKKEMLELFRDKRTLFTTIILPVILYPLLFVGFSA
                     IMSRQVDVLEKRGATIAFQDSLSFRTPETLAIRDSIYEDLKKIENFSIIPAPPALEKL
                     YQEGEIDAIVTVKDSLNVLQEPVLIVSIRYDGSGEKGMMVYHKLENRLLETSQKITAQ
                     RLEALHIDQQTLKPLEIKPIDTSTAEKKMGSVLGAMLPYLMILLLITGASVVAADLVA
                     GEKERQTLETLLVSSVSRGEIVLGKYLTIVTMAMVNVVINLVSISFSIQYMLSQTGLD
                     L"
     gene            complement(31535..32302)
                     /locus_tag="PK015_01965"
     CDS             complement(31535..32302)
                     /locus_tag="PK015_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOL33273.1"
                     /translation="MIKVQSLVKEFIQKDGTSFRAVDEVTFDASNNEIVCLLGVNGAG
                     KTTTMRILSTIFKPDSGKAEVEGYDVVTEADKVRENLGFLSGDTGLYQRLTPREFITY
                     FGRLYGVPEGMIEHRIREMSEILDMNEFLDKKIEFLSSGMKQKVSIARSIIHNPPVMI
                     FDEPTAGLDILTSRNIVSFIRDCRNQGKCVLFSTHIMREAERLADRIVMIHKGRVLKQ
                     GSLVEMREETNLSDLDDIFIYYINQANGELKVAANEF"
     gene            33021..33770
                     /locus_tag="PK015_01970"
     CDS             33021..33770
                     /locus_tag="PK015_01970"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOL33274.1"
                     /translation="MRDPFSILNTLEAAYVLDVATGRGDFINILRDNLKSYVKIIGVD
                     ASDKNVDRAQKLFPENDIEIYQMNLGALRLDDNSFDLVTIADSLHHLDNLEAVFSEMM
                     RVLKPGGSLLVIEMYSDGDQTEAQKTHIMLHNWLAAIDRKMGVFHNNIYPREEILSLV
                     NKLGLDDLIIDDFYYPVDNPKDTRNCESLRKTCQKSFKRLESLEDNEELIAEGNSLLD
                     RINRIGCASASKLLIMGKKPLSNKKENHKEK"
     gene            33771..35030
                     /gene="rho"
                     /locus_tag="PK015_01975"
     CDS             33771..35030
                     /gene="rho"
                     /locus_tag="PK015_01975"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOL33275.1"
                     /translation="MPIEENLFEMSQLQLTRLAKKLEIPGYSQLKGKDLVFKILEFQA
                     AQEGLAFVTGCLEITDDGFGFLRFPENNYLQGKDDVYVSQTQIRRFGLKTGHMVSGPV
                     RSPKEGEKYYALLRVEAVNGMSPDSVQNLRSWDELTPYYPTERLNLEFDPHNYSTRII
                     NLFTPIGKGQRGLIVAAPRTGKTTLLQNTANAILTNHPEVHLIVLLVDERPEEVTEMK
                     KILKPGNREVISSTFDESPKNHAAVSEMVLEKAKRMIELGQDVVIVLDSITRLARAYN
                     NITPSSGKVLSGGIDANGLIKPKKFFGAARNTEEAGSLTIIATALIDTGSKMDQVIFE
                     EFKGTGNMELVLDRTISDMRLYPAIDLVKSGTRREELLLTKNELNRMYVLRQYLKTLS
                     PVQAIETLRKKMQATSTNAELLNSMSN"
     gene            35052..36170
                     /locus_tag="PK015_01980"
     CDS             35052..36170
                     /locus_tag="PK015_01980"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOL33276.1"
                     /translation="MPYKLRVFLLTALLIALPLMGYSAGLSKDFQILENKFQQGDIAG
                     ISSLLPTAKANNDEDRALQLYLSAMLKKTENETISSLQQAANLYPKTYYGQMSILELA
                     KYHILKREINQAEELLHKITNVQLIEKFYWLAYCADSKSNYDKAISYSENYLRMTSDN
                     KYLENTYYIIANAYRKQGKYQSAITTLEKLKKIEGYPKNEQYFYYQLGYCYQLLGNVA
                     DAYQNYKKAFELNHNSPLAFMAEDKLFELRNRYNPSLDLSFLYPYTTLDIPGLIQPEI
                     TIPPAASIIQDLPLKLENRPETGYYVQIGCFSSEANARKRTEEIRGLNISAIYFEDKN
                     KKNTPWVVASGPYSSLTEAETLKSILAQKSVDSFITRY"
     gene            36215..38809
                     /gene="mutS"
                     /locus_tag="PK015_01985"
     CDS             36215..38809
                     /gene="mutS"
                     /locus_tag="PK015_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOL33277.1"
                     /translation="MIVQYQKIKNEHPDKLILFRMGDFYETFFEDAYTASKILNITLT
                     SRDKSDENPVPLAGFPYHALDNYLDKLIKAGIKVAICEQMEDPKQAKGLVKREVVEII
                     TPGTVLDHNLLGGTANNFLSAIYYEDENRKIGLASLDLSTGEFIFTELSKNELANELL
                     KLKPSEIIVNGSDSEAFIKNLNLEYEPTITIFDNWQFRPSEAANVLKKHFQVHSLEAF
                     GALGKNMGVTAAGAALAYIQSLTSDPLSHISSLRFYSLDNFMLLDEITRRNLELTHSL
                     RYNDRHGSLLYVLDQTQTPMGSRLLQNWLLHPLLDKEQIIKRQDLIKAFLNNTAYLKD
                     VRTILHNIGDISRLVSKLGALRINPRELLALNSYLLTVADLKKKLIAFNHPLLDNFIP
                     ALEGFEEIIEIISKAIRPQPPITITEGGIFNQGYNPDLDELLEIIHDGKSWIARLEED
                     ERNKTGISNLKVGYNKVFGYYIEVTSSQKSKVPDYYIPKQTLVNSERYISPRLKEFEA
                     KVLSSEEKVKNLEYELFKQLRQQLAGYLEKLQSLSETIALIDVQSCLAWLAWQNGYCC
                     PEFTEQRTLKIVEGRHPVIEKLLTDTDFIPNDTELDYPQTTIAIITGPNMAGKSTYLR
                     QVGLLVIMAQMGSFVPAKQLQMPIFDRVFTRVGASDNLAQGQSTFLVEMIETANILNT
                     ATPDSLILLDEIGRGTSTFDGLSLAWAIIEYIEKYKHSLTLFATHYHELTELESIYPD
                     IRNYNIAVREYNEQIIFLRKIERGGADQSYGIQVARLAGIPEKVIRRAKEILKNLEEH
                     EISPQGLASSLRKKLQETSPQMELFEVLAEKADELDPIINEIKDLDLDSLTPIQAWQK
                     LKELQDKL"
     gene            38806..40512
                     /locus_tag="PK015_01990"
     CDS             38806..40512
                     /locus_tag="PK015_01990"
                     /inference="COORDINATES: protein motif:HMM:NF025009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOL33278.1"
                     /translation="MKKHLWKIIPLIVFALFAISINGAQNNPELAGVINGTPIRYDEF
                     IQELLLNYYNFQYENNRAPDSREKELIRQQTWEEKTMYVILKDYYKKYNIKVTAEETI
                     EYLKSNPPETLKKSPLFMSDGKFDQKIYVQSLLYDTPYNLKPYRDRYQNYLIPNLILQ
                     DYIIRDELLTSSEKKLITKILQSSANIKWTILDTESINPYITEEEIQIYYQKHLSDFK
                     LKPYINLAYTTISVVPSKTDIRLTNAIADSIYNELMRGKTVQDILESELPIAPHLSFI
                     SMDYVRESELEPSIYTILSQLKEGEFSPPIADKEGLNIYQLESRTKSMCSYNVLRIPY
                     IPSQSSIDVVLPTAQQVVKLARKENLSIACDEMNLDYQKTGIISPDYDWFGDSQVVNN
                     ILNLIPNYSDRYVFDPIYSPKKKVWVVVELLESSLYSYQSLDEVRPQIKKKLEEQKRA
                     ELALQLADKISSGQSTIPSDAPTIYIENMTINTPLLDNDAANIYYQTLRCYFKKEKPK
                     AFIIDEKIVLPEVISVKIDKKKKISQDQIIQVYKNNLPPDWFNNWMASKVKKAKVKVY
                     IQ"
     gene            complement(40547..45997)
                     /locus_tag="PK015_01995"
     CDS             complement(40547..45997)
                     /locus_tag="PK015_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOL33279.1"
                     /translation="MKKILVFLMSLLLLQTCLIAENLEAEKLISIVKSDNHQTKLKLE
                     STQLTLKESIQNNQEVKEIKLKGVENSTVFNGMPELPILGTFIAIPPQGNFNVTFTYD
                     SVDIIPLNNPKLYYAEEGALPVTIEAQNLFPENFVTFSEPAILRDFRVIQLNIYPCQY
                     DFTKQQLRYYHNIQVHINYTTDKGINELPFESYDNYSYVFQNIYEAQIANFDEYRNPL
                     IAPEQGRILLIYGNTTDANFLTKLNEFVTWKRQKGYEVNVVSTAQTGGTSNTSIKNYI
                     QNQYDNPETRPDYIILLGDTTGSYIIPTWYENYSNYNGEGDYPYTHLAGNDTLGDVFI
                     GRISVENLSQLATVFNKIYTYEKNINISADAASWLNRMLLIGDPSSSGISVIYSNKYI
                     KEMSSKIHPDYTYIENYTSSCSSTMNSGINQGVSFFNYRGIINMNGWSPSESLNNGPR
                     LPHSVILTCGTGGFAGTSTTETFVRLGSEAVPKGALTAIGMSTTGTHTMFNNALSCGI
                     FDGIFLYGMRNIGEALLNGKLYLWNVYGTANTSQANSFAHWCNLIGDPTVEAFVGIPK
                     GLLINAPDSIPRGCSLVDINIMDLFQNPMEGVSVTLYNSVYQNVVSKGYTDETGTVTL
                     QIPSFIASNLLITASQHNCKPVQKTLSLNQEGSLVYFEKYIIDNGTAGSSGNGDSFIE
                     AGETIALNVEIKNTTTHIISGLQGILSTTNPYINIQQAQATYPDIVPEDTGVNNTYFL
                     LHFANNIPSFLNARFNLELTDTQQNIYQIIFHMGAFNANFNVNNRYVFAGCDTILDPS
                     ETGILTVSITNNSICGISDIYGELQSMNDLVSVPDSISYFGNVLAGMTVNSIDGFSIM
                     ARPQLIPGMIIPFQLRLYNSTGFEQLTYFNLPIGTVSQNTPLGPDEYGYFIYDVSDTA
                     YPDCPVYEWIEIVPSLGGSGTLITGLNDSGLINDEGDQVGSDVLETINLPFTFPYYGK
                     EYNQITVCVNGFIAFGVTENGEFRNGRMPGGQGPSPMIAPFWDDLIILSNAGIYQYYD
                     IVNHIFIVEYHNLKNGFNRTSEETFEVIFYDPHYYPTSLGDGMIKFQYKTFNNVDVGG
                     GGCSPSHGKYATVGIRDHTNRRGLEYTFNNQYPLAAQPLTNNKALLITTVPMLQERPH
                     LVIGDFILQDDNGNGWMEPGESGEIGIKLNNLGIATATDVHIIANTVNPQVLIQNAES
                     DYPDIPGSGSAVNIDPITISISPECPHNTTIELNLTVSISGNSWNYIQNITVKKPQLS
                     LSGVYINDLQGNINGMADPAETFNLIVHYTNDGSVAAYNITSNISCMSSDVDILNPQQ
                     LINYIPAGATCQVIYEITLSPNVSVGNNLTFYITYLGEAIEAHNETIRLNVGTTGMYA
                     DFENNNGSFVTQPANNAWQWGSDSIVGAHSGTKCWGTLLNQQYPNYATWTLTSPVIYI
                     GPNFVLEFWHWYDMEQGYDGGNLKISTNSGGSWTLLTPEGGYPYQNVSALSGPGYTGN
                     SQGWVLARFNLSNYANQNVMFRWTFSSDPGIQGWGWYIDDVQTTGYIPFAGKLYGIVF
                     SSNPEINYSQVLIQNSTLLTSQPDSDGNYVLYLPGGLQSVNAIAPGYQTETLSSININ
                     LQNPAINHDFFLAYFAPATNLSFRVVQDSLYLNWSAPEEPFYPILNYKVFRKLNAGNY
                     ELMGIYSQTYYNEQLSTIGNYRYYVITCYETGESVGTEPISFSFPYVDITEQNINPLV
                     SKLYQNYPNPFNPSTTIRFDLAKGGPVKLSIYNIKGQLVNCLVDDYLREGTHTIVWNG
                     KDKQNRSVSSGIYFIRIESKEFNSVRRAILMK"
     gene            complement(46064..47077)
                     /locus_tag="PK015_02000"
     CDS             complement(46064..47077)
                     /locus_tag="PK015_02000"
                     /inference="COORDINATES: protein motif:HMM:NF019912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33280.1"
                     /translation="MKKYIILITAILVLLLAGCAKRNSPWQNEDMLTLKRHIEVVGNP
                     LDISFDTENIYVALDQGGISIINSANYSQKWYTILSSSDESIIPLFKIRKIGVVGEYN
                     RLFLNETDATDRILIVDNSNLDSLRIIDSITGATQDIQDMKVQKIINPVDDNVIEIIY
                     SAGRNVHYGKYNGTLWLGSTFSIYPPASASGVDLDDRYVYIAAQQRGLLIYDRNSLEM
                     ISEIAVPGEAQKVKVVGNYAYLACRQGGFTIVNIADRTEPLLVSNFDTTGYATSIDVK
                     DNKAVVSSGGGGIYLFDVSSPSHPLLKQNITSAGYTNNAKFYNNQLIVAARDKGILIY
                     EMD"
BASE COUNT        15245 a         8946 c         8242 g        15004 t
ORIGIN      
        1 gcatttataa tcagttaagg tctgtttcgg ttatatggaa tgctaaagag tttgagttct
       61 aagcggctat atttttagcg ttgattatgc tggttaaatg tagaccctga cttctgattt
      121 cacgaatgaa actctggaga ttggatagtt ttttctcgtt atggcaatga gtaataatta
      181 ttattggttc ttttcttccc tgatatttat ttaaagaaga gattttatta gctaaggttt
      241 gttcagtatc atcaggcaca tcaagaaaga tatcccgttt cagagctgga taaccacgag
      301 aataagctaa gctttgagct aaggaatttt tactggtagc actatccaca aaaaagagat
      361 tatgcttatt taaccagtct aaaattatgg tcataatttc tctattttct gttacactgc
      421 ttccttgatg attattagct ccaactgcca taggaagttg ggaatagaaa tcatccagca
      481 gtgcggttat ttcatcagcg gaactattat ttgtaataca ttgcttttcc tgaactccag
      541 ttccttccat aggaatgtga atcaaaacgg gatgaccata ttgggaagca atttcaccgg
      601 cttttctggt gtaaggaaga ttgggcagaa cagcaaaagc aacttctgca ggtaattctt
      661 gagcaaacct ttccagaagt tttccattat agccaaagtc atcaacaata attgccacat
      721 caggaagttc agaactgttt aatttagtat agggataagg agttaaagga acattagcca
      781 atgttttaga accattatcc cctgaattta aatcagttaa ggaatctatg gaattgattg
      841 aagattccga tgaagcaagt tcagtttctt cactttttcc cctgtttata ttctcttttg
      901 gagagaagaa cttatcacaa gaacaaagtg taaaaaccag cataagaaga agaacgagag
      961 gtataaaacg agatagattt ttcattccca ttgaaccagg tctccaattt ttaaattatg
     1021 tttttctgct tgtccagcat tgagctccag aacatatcta tagattccat taggtgctat
     1081 catttcttca ctgaaaggag tattattttc tgcaatatgc ataatttttt tatctttatc
     1141 aatgaaaatg atatccagag gaatataagt atttttcatc cagaaaggag tttcagaagg
     1201 acctttaggg tcaaagagca ttcccctatt ttcagccata gattcacgat acatcaaacc
     1261 ttgagtacaa gcagcttctg tagcagcaat ttctatttca tagatggctt taacttctcc
     1321 cgtaggtgcg gtaatggtta aggttgcatc ttttctaaat tctgttcctt taatgctatc
     1381 aaacgatttt tcttctggtt tagacttgca tccattaaag cctaaagcca gacagaagac
     1441 cacgataaaa agaataccct ttcgcattga ttattttaga agtatcatct tatgagtgat
     1501 agttttgttt ccactttgca aggtatagaa atagactcca ctgctaacag gttttccagc
     1561 ctcatccaaa ccattccatt gcactctatg aattccagaa ttcatttcac cattgattaa
     1621 agtttttacc agctgacctt ttaggttata aattcccaga aaagcttttg ctcttacggg
     1681 caaggaaaaa gtgatgtaag ttataggatt aaaaggatta ggataatttt gggaaagatt
     1741 gaaaggtata acaggattga gagggtcatt agcatctgta tcagaaagaa tagtgaatga
     1801 actatctcta ccatttacac tataaaagaa gtcaatactg acagctgttg tctgataact
     1861 gatagtgaaa ccttcggtta taggtgaacc aagattgata taagcatatt taccctgttc
     1921 aggattgaag gcactgcgat agattaatcc cagattttcc tcaatggtta aattggataa
     1981 ggaatcagac ggaacaagtc cccaatctaa acactttaaa ttattctctg gaaggatttg
     2041 aaagacagct tctacggtgt tattttctcc tgtctcatcc atagttaaag tgacttctgg
     2101 caggttatcc agttgtgcac cttgaacata aaaacgaaat tgaggattct gaatatattg
     2161 agccataaaa taggcatcaa ccgagttcaa aatatcattt ccatccacat ctgcagcagc
     2221 aatacgatta ttttcccagg gaaaaggatc aatttcaggt agagaatcat aaccaactat
     2281 ataatccaaa agcagttgta catcattcat tgatatcata cgatctccat tcacatcacc
     2341 atatacaatt aaattggcat ctattccaga aatctttata tcatcaagat agataccatc
     2401 tgcagtaatc ccataatcag aggtaagctt aaaccggata taaacatttt gtccaacaaa
     2461 ctgagaaaga gaataagaaa aagtggtcca gctactttgt gtaccagtta agctaccgag
     2521 gttaatccag ttagaggcat tatttgaagc ttgcacatag acaaagtcat atccattttc
     2581 cagggcatag atagttttaa aacttaaaat tggatttaat atattttcca gtgagatagg
     2641 attatttaat tgaagtattc tggtaacatt atttccataa ttcccatttg gagaatcagt
     2701 aataacagat tcaccctgat aggtaactag cccccaaggt cctgaagcag tccaattaga
     2761 taagctttca caagggtcat agaacagcac taatggctca atgaaattaa agacgcagtt
     2821 attattttct gtagtaagta aaaaatcagt ttcatataag gttgtattag cattactaaa
     2881 tgttatatga taataacctt caggaatttg cataaacata aagcttccat tagtatcagc
     2941 ggtagaactg cgtggaggaa ccgtattcaa agtgacagtt aaatttggaa ttccataacc
     3001 ttccacatca cgaatttgac ccattaaatc tacggttgca gcaggagaaa ggataaagtt
     3061 gtattcagta gaaccagagg caggtacagt aatttgagca gtttgaggaa tatagccata
     3121 ggctgatgca gtgagagtat aatttcctgg gagcaataaa cgatgataat ctcccacatc
     3181 ggggtcagta tgtatagctt tatcgttacc ctggacagta attacggcat ttaagggttg
     3241 ccctgtagtt gaggtgacca aaccatgaat tcctttatgc acaaattcca tataggaaag
     3301 catagattct tgattcaatg cccagaaaga aggaagttgt gaagcgggag gccatttgtt
     3361 ttccccaatt tccattgtaa tatccatatc atcagtgtaa ccataattcc agtcctgcat
     3421 tgagccagtg ataacatacc aggcggcacc attagtgata ccatgcggaa attctgtgct
     3481 attatacata gaagaattgt gactggtata ggttaaagct gcttgaataa atacatcatt
     3541 atcaggtgtg agagtataag tgtaatccca aggataattt gctaccaagg caccaccgtg
     3601 aaaattagca gaaaggacaa aatggtgtgc attggagaaa tccattatag caatagtttc
     3661 tggagcccat tgattaccat ctggatgctg aataccagta ggcataggaa aatttctatt
     3721 aaggtcaaca ccattagcat tataacgttg ccctagaaca tagccatcag gattcatcat
     3781 tgggcaaata taaatttcag tgctattgac taaattggtt atacgaggat caattccata
     3841 ttgagtggtg agcaattgaa tcaaacgtat gcacatatca tagcccacaa cttcgtctcc
     3901 atgtatagaa gaaacatatt tgaattctgg ttcagcttct tctatatctg gattatccgt
     3961 tatctttaaa aagtaaatag gtctgccttg aactgaggtg cccgcttgaa tcaaagagca
     4021 gatattagaa tattgatttg ctgtatctat cataaattgc tggtattgag tgatagtgta
     4081 gtaggtatct ttagtatgtt ccagagaatc tatcttctgc aattccagtg cattctgtct
     4141 ggctaaatca ggaagttttt ctgctttaaa tccattattc agcaatttat caaattcatt
     4201 atcatctctc acatagacaa taatagtgcc agtattacga ttaacattat caacgctaat
     4261 ttgaagggaa ttcagtattt taacatcaga atctatatcc cagctggtaa tatgcacagg
     4321 atatgaaaat gctatagctg aactcacaag gaataatgct ataactaata tttttttcat
     4381 ttatgtgtcc tattcttata tacatataag gttcaaaaac tatgcaatat attgacctat
     4441 actttgaaaa tgccaggctt acagttgcac ataacctgcc ttgcttaatg ctgcttcatt
     4501 cctgacctga catatttcac aagcggttat tgcactgtgc ctggcattat tcataaaaaa
     4561 atatatcctt tttttgtcaa ttcttttgat tcaattttct tttattcgga aagatattca
     4621 tcgcataata cggataaata tagaatttga ttataagaaa gatatattgt cttcaattat
     4681 ggatattata acttactgat gaataagtta tgtccatatt cggacataag ctggacatga
     4741 gttggacatn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt ggacatgagt
     4861 tggacataag ctggacataa gctacacatt aactggaagg gaggtaataa ttgtaagaca
     4921 agatggatat aggagatttg aaagaattgt agctgaggga taatttatgt aactgcattg
     4981 aattgcgatt taaataaatt cattaataag gaggtaaaat tccctttttt atttcagcta
     5041 aagaacccat aatagggaaa taattatata attatctaac ggatatacaa taagttatgg
     5101 atattaaaat aatgtataag gtgaaatcat tatatataat tataaggaaa tcttattaat
     5161 atatttatct caggtttata agtttttctt taaaggttga gttctatcca tatacccaga
     5221 ctgaaagtac gaacacctgt atctcgttta ttatctgatg attgatccca ggttccggtt
     5281 aaacctccgc gaatatcagg attaaattga taggtagcag aaggactgat agcaaaatgc
     5341 gtatagtcac ggtctacttg agagctttct cttcctttag ttttatcata atttttttca
     5401 taaagaacag ataagctgga aacaagttca ttattaatat gaattttctt gtgggtaaag
     5461 ggaacagtga atcctttccc ctgagtgaaa gaataagaaa tattgccatt tacagcatgg
     5521 gtaagattgg tttttactat atcataagtg tccatatccg tggtatttgt tccggaagta
     5581 atagaataac tgatattagt attgactttt ctaaagaaag tgccggtaaa accaacgagt
     5641 ggatttaaag ctaaggaaac agattcttgt ttaggttcta tccaatccaa atctccagtt
     5701 gctctggtag tatattgcag tcccgtgttt aatcttgctc cactaaggaa tttattcatc
     5761 ccgaaccaat tttcaaaatt gataaaagat agggttaaat ctggaaaagt gataacttca
     5821 gtttgatttg aagcatttgc tcggtttttt tgtaaactat acgaatagga gataatgcta
     5881 tcaaaattac gggaaaaagt gatgccactt ccgatagaaa tagtgttatc atcgctaaca
     5941 gaatccagat atcctgcagg agtagtatga ggtaatccaa gttgaaagag gaaaggtggt
     6001 ctatcatctt ttcttgaata ggattgtata tatgagtttt gataagtcat tgtgatattt
     6061 ttgactttag aaaaaaggtt taaaagagtg acaggaaaat aggtttcctc ttttccttta
     6121 tctttatctt tatctttggg gggttgagtt aaatcattat tatctttaaa gcctggttct
     6181 tcggttggga tgtcttcttt tccaaaatag atttcttctt taaatccatt ttcataacct
     6241 ttcccattat ccccatattt ttcggtttct tcttgttgtt ttttccattc ttcttctttc
     6301 ttctttttat cttcatccga aggagatttt ggttctttat cagagtcctt tatttcatcc
     6361 tgctttttca taccttcttt gcgagaatga gaagaactaa gattaactgc ccaagatgat
     6421 aataaactgg aatttagcag tgtcaaatta gttctgatag ttctattaga gtttccatca
     6481 ctctgataca ttgtaatttg tccctcatca gtattctgga aatatttcct ttgtgaatcg
     6541 gaataacgcg cacttgcact ggcagttaaa ttaatataat tcctgagata atttggattg
     6601 tagttcaagc ctaaatcctg cacataacga gtcatcttcc cgatattaat atcttgccag
     6661 taaatcttct gtttgaggtc tcgttcggta tttattcttg ctgaaagaga aatgtctgaa
     6721 gtaagccccc agctgatatt attgtcagaa gtaaaagtct tggtgggaac tgtttgagtt
     6781 cgcggatacc aatcataaac cccttctctt ttttcccagt tataactttg cggttccgaa
     6841 ctactcaggg taaagctatt attaaaagta ctgggaaacc accctatacg gtagttttta
     6901 aagagaataa aactgacttt atcactagga attccaagat tgtagtttaa agttccgcgc
     6961 caattgaaag tggtatccac tgttgtagga gtgtatcggt atgatttctc aatacgacca
     7021 ctgatagaag ttctataaac agtatagaac aaaaaaggag atttaggagc ggtacgttga
     7081 ctgaacccga aatcagcagc ataaactaag ttttctgttc tttgtctatc tttttccacg
     7141 gggtctaaaa tattatcata tagaaggtca gaattggcaa ggaatcttgg tatacccaaa
     7201 ttataattac gagataaact taaaggaata tctaaacccc aggcattagg aaataattta
     7261 ttcaggaaaa atttattcat gatattaaga gttttagtgc tggtaaaagt attggttcta
     7321 cctcgttgta taatagtatt gaagttttca cttttatcct cataatctat atccagtgta
     7381 attacatcag caaaggcaga attcagtgag agtctttttg ccacaccgac atcttcaaaa
     7441 ggttccgcaa ctcgcatatc attaaaatag atggtcccat tatagggttg gggaatattt
     7501 aaatcatctg gaacatacag tcccaaataa atatctcgca catttgttaa agtaggtgtt
     7561 cctttaaacg aatagatttt attatcttct ctgtattcac ctgaattaac atcagggaac
     7621 atttctttaa gttttgtgaa atcctgcagt tcataagtaa attgagtcca gtaactacga
     7681 ttcattttgg gtagatagtt aactacatga atgcgttctc ttacctgata aaaattcaag
     7741 ctatcagctc caatcctgaa gattatatcc agagaatctg gattaatagg agaggatgaa
     7801 gataattctg gatacaccca gaactttatt ttattatagg aaagcagact ataaggttca
     7861 aataaacgct gtcttaaaag gacctgttgt ccaggttgta agttttgaat atccagagat
     7921 agagcagatt cacttgattc tcttcgttct tcaatataat aagttcccgg aggtgaagtg
     7981 taatgagcac tatttctttg attattaacg ataccagaaa gataggagat attataggta
     8041 ttgataatat tactcggtac tatatgtccg ttaatatcgc gcacatagaa atcttcccat
     8101 ttatttccga ccacattaat atcgtataat agaaccttta ctggcacatc tgtttcaaca
     8161 actattctgc cataggaaat tttctttagc gttggagcag ctccagaagg agcattatta
     8221 acaatttcgt atactaaagg atcagttaag ggaatacgat aaagacgcca tccatattca
     8281 ttttcacttt ccagatattg tgaatcagta agagaaatgg agtagctaaa atacctatct
     8341 aaagtattaa gaacgccatc attattcaaa tcttcggtat ccagcacctt atttccttcc
     8401 gttccattaa taaaaggata atctccgaac tggtcaatgc tgttgtctgc atggtcatta
     8461 gggtcgtctc ctggatgacc atctggaatt ccatccaaac ccgtatcttc ctctaaagtt
     8521 agaacaccat catggttttt atcttccgta ttgagcacat ttaagccacc atattcagtg
     8581 taaaaatctt cattgatatc tcctaaatca atacgcaaaa taggatgagc aactgggtca
     8641 ttagctttcc tatcaatttt aaccattaat tcaatatatt ttttttgaga aaaatccagc
     8701 tgattaccca gatatttcat aaccccaccc caactccaca cttcagagcc ctcaatacct
     8761 aaatttgcag gaaaaacctt aatggcaaga gctgtaacag attccttttt ttccctatct
     8821 gttaaagtag caggatcttc cacttgttct ctatgaatat ttttaggatt ataccagata
     8881 gtccttcctt tggcgagact tgtattccag ggtttacttg ccattgacca ggaacctatg
     8941 gttactccta aaggataaga atcaattatt gcttccatat cgtctatata ggcttcattt
     9001 ttctttccat gagggtcacc atagatatta ggtatggtat aagctatttc acctgaaagg
     9061 gtaatgcgtg aatcagcagt agtatttatc aagggtaaag catttaacat tctcgtaata
     9121 aatgtaggtt taacagttaa gctcccatca atatctgtca tatacatttc aatgttttca
     9181 ttaccaattc ggggacgtct atcggcaaca ttttcagatc gataaataaa agttccccca
     9241 agttttgccc aatcagttag attccaatct gctctgactc cggttaaggt cttactggaa
     9301 atatcaaaag tgcttctata ttcataatca atttcaattt tagcatcggg atcttttccc
     9361 gcagaagtca aaaaagtgat tctaccaaaa tcataatcta ccagataatc cacattctct
     9421 ttctgcttga tgccgttcac tttaacggaa acactgcctt taagtatacc tccctgggat
     9481 aaatctacat attctcgtcc tattttaccc ttaactgaca tataaaatga tatatccagg
     9541 taattgatac tttcaccttc atccgtgtaa atgataccat cacccagcgg gtcaaaaggt
     9601 tgaatgaaag gtatggtaat gagaccggta gataaattga ctgtattgtc atcaccatta
     9661 attaagccgt cgccattgct atccattcgt aaataatccg tataagtggt aatataactg
     9721 gtagcaagag aatctggaac cagataatta cgggtcaagt ccacatttaa ggtgtaaata
     9781 tctaaggtaa agccatcact tttaatgtta ttcttgtgca tatcatagac attacgcatc
     9841 tgatagtgcc agacattatt tgggtcatgg gggtcatatt cttgattacg tcttcttagg
     9901 actttaacat gtagaatccc atcctgagct tcactatttg ccggaacagg aataccatct
     9961 ttttgagtat attttacagc aatagtagct ctccggtcaa cattactatt aatgcgaata
    10021 ataccagcag aataatccgt gataaaatcg gttccttcaa ttaattcatc ataatagggc
    10081 acataatagt ttgtagggct gaaaaatata gtatccccaa ttgctgctgc cacattatta
    10141 tttgcattgc catcatcaat aaaaagccta acagtaccgt ttgcgggaag taaattaggg
    10201 tttttaatta tccaagcacc cgtagggtct gtttttactg cattatttac ccaacctgca
    10261 ggtacattgg aagaaacatc agcttgagtg taaagttcat atacatcata aggatttgcc
    10321 agataataca tagttctggg ggcataatct ctactacgaa agatagtaga atccgcctga
    10381 ctggttccta cataatgttg agtgtttttt tgtccttctt ccgtactggc tataaaagtt
    10441 aaatccagat ttttgtattt tagttttgag gttatcccaa agagtccctg tgaagcagca
    10501 gaatagctta tataacgaga acctggtaaa gaaagagcta tatttccacc ttcaatagtc
    10561 tgaaataact catcttcggt accggtgtat tttatgttga tattatcagg gtcaaaaaac
    10621 tgctggtcct gattactatt atattttaga ttaactccaa tcttctcacc aatggttcca
    10681 gttaaacgaa gattggtatc ctgtctcatt ttaatatcca gttgactggc tgtattagtt
    10741 tcataaatag ccacattctt tctttttgtg ctgcctactt ccatactgat tttttctgtt
    10801 ccatctatgt tcaatcttcc ggcagaagaa cctaaaactt tttgcacagc tttaggaacg
    10861 gcaatagagg gaagatcaat ggaaaactct ttaatcatac cggttttagt aacggtagtc
    10921 tgttgagctt gagttaaata aagggaaagc atagacttat aaaaggcttt ttcttgcaga
    10981 ttggcaaaat aggtgtcaaa agaaagaatc cggggtggaa caaggtcaaa atcatctatt
    11041 ttagtatgaa taataacctt ctcctcagga taatttatct gttctttggt gtcaggttca
    11101 aattccctta tccagtaggg ttggctcttt atataattca atcctttata taatttataa
    11161 acatccactt tattataaat caaaggatga taaccttcca gatagatgcg aggttggggt
    11221 tgttctttag caaaagccag aatgataatt gtcaggatta tgaaagtgag acaaaaacgc
    11281 tttaacatca aattaatgac ggatggtctt gtaagccaaa taaaaaccgt ctaaggtaat
    11341 ggttttatct acaatatcaa cagaaggaac caacggataa actaaatcag caattccacc
    11401 ggtcaatata gtaacaattt tttgacaatc agcatagtgc gtcttaatct gttgaatata
    11461 gcattccagc atataagcat gaccattaac tatccctgaa agcaaagcat cacgtgtgtt
    11521 tgttcctaac agaacagagg gaggaactat ttcaatttga gaaagtagag ctgctttttc
    11581 aaatagggca tctgcactta cttttaatcc tggagcaata gcagtccctt caaagcttcc
    11641 ttctttagtt ataaattgga tagtagtagc agttcccagg tcaataatga tacaatccga
    11701 atcatatttt tttaatgccc caaaagcatt tgctattaaa tcagcaccaa tgaatgaggg
    11761 atcgtccact ttataactaa tacccagcgg actgagtgca ttaatctccc agacctcagc
    11821 actaaagtat ttttgaaata gatgcatcca gatacgcatc agttctggaa ctacagaacc
    11881 aatgacaata ctattaatat tattaaggga ttcagtattc agaaggggaa tgagcaaact
    11941 gaaatattca tcagcagtac gatgtgcaga actgtaaaaa cgtgcaaatc gtaccatttc
    12001 atcctgctgg tatataccac aggttatgtt cgtatttcca atgtccacca ccagcgtagt
    12061 ggtttcagaa tatttctcca tattaataac cacacaaaga gtattttgga aatataaaaa
    12121 acatagccta tatcaaatta atggctacaa ttgcttaaag aagatttgtg tcaatataat
    12181 tcctgctcct gaatagaaga aaagcaacgc tacttaaaac caagttcttt caaatcagaa
    12241 ggttttttgc gccatttagg tttaaattta acccagagat gaaccataac tggaacgccc
    12301 ataaaattcg taagttgttg ttcggcataa tctctaatct ttgccagacc agctccattt
    12361 ttccctatga taataggttt ttgactttct cgttccagcc aaatcacggc atcaatatgg
    12421 attttgtcat ctaattcttt aaatttttct accagaacag cagtggaata gggtatttct
    12481 tgactaaaat aattaaagat tgcttcccgg ataaattctt gagcgaaaaa ccgcataggt
    12541 aaatctgata gctgttcttc actgtaataa ggaggatgat agggaataaa ttgtagcata
    12601 gcctccatca gttcaggaac tccatcacct ctggtagcag agataaaaaa agcttcagaa
    12661 caggattctg gtaattgatt gataaagaac tcacgggaat aatccttttg caaatctatc
    12721 ttattaaaga cagcaatttg tggtataggg atttttttaa gaagctccag cacttctaaa
    12781 tcataatccg ttggaaagct gataatatca ctcataaaca ggattaaatc tacatcttta
    12841 tatgattctg accataaatc ctgcattttc tcttgcaatt tatatcgtgg tttgagatag
    12901 ccaggagtgt caataaagac aatctgacaa tcatcccggt ttaaaattcc tttaatatta
    12961 taacgagtgg tttgtggttt tggagaagta atggaaagtt tttcacctat caggcgattc
    13021 ataagagtgg atttaccagt gttgggttta cctaaaatgg taacaaaacc gctacgaaat
    13081 tgaggattgt ccataaaagg aaaataggag gacagattta tccatccgtc ctcattaatt
    13141 tagtttttaa tagcttatat ctcattaaac acatttttaa taatttctgt tacttccatc
    13201 aactggtctt ctgtaataac cagaggaggt gcaagtctta taatatgacg atgagttggt
    13261 ttgcagagca cacctttttc tttcaattta agacaaatat cccaggcttc atagctattc
    13321 ttcggttcaa ccacaatagc atttaataag cctttacccc gcactaattt tagcataggg
    13381 tgctgaatgt cccttagtgc agtgcggaat ttttcaccca gcacaaaaga acgctgcgcc
    13441 agattctcct cctgcaccac ttctaaagaa gctttggcta cggcacaagc taaaggataa
    13501 ccaccaaagg ttgagccatg ctgacctggt tttataacca gcattatatc cctgtctgca
    13561 agcacagcag aaacgggaat aacaccacca ccaagtgctt tacctaaaac cagaatatcc
    13621 ggtctaactt cttcatagtc gcatgctaat agtttccctg tcctgcccag tcctgtttga
    13681 atttcatctg caacaaacag gacattatga accttacaaa gttcatagca agctttgaga
    13741 tatccatctt caggaactat aacaccagct tctccttgaa tgggctcaat gataaaagct
    13801 gctacatttt ctccatactt ttccagacaa ttcttcaaag catccacatc attataaggt
    13861 atcttcaata taccaggtag atagggtcca tatccttcat aacaatcagg gtcagttgag
    13921 gcagaaacca ttgtcatagt ccggccatga aaattatttt ccgcaaagat taccagagcg
    13981 ctatttttct ccacgccctt aacctcataa gcccatttcc gtgccagttt gaaagctgtt
    14041 tccactccct cagctccaga attcataggt aataccatat cataaccaaa gtattcggtg
    14101 atatattttt caaattcacc caatttgtta ttataaaaag cacgagaagt aagagttagg
    14161 gttttagcct gctcaattaa agcattgatt atcttaggat ggcagtgtcc ttgattaact
    14221 gcagaataag cagaaaggaa atcgtagtat ctattccctt cggggtccca gacaaagact
    14281 ccttctcctt ttgccagcac tactggtaga gggtgataat tatgagctcc atatttctct
    14341 tccagttcca tagcttcatt tgaactgata gtgccataca cgagattgtt ggacatttta
    14401 ttctcctgtt ttatttgatt ttctttcaaa agccacctca ttagttcaag tgccctgtgt
    14461 caaggacatt cttatcttgg tgcaaggtta aaagctttta tataaacgat tctgactatg
    14521 aaagcagctc attaatatgt tttagagtat cattgagagg ttatcaatcg ctttctacct
    14581 aaaattacac acgacctata gaataatatt taaaaccaag ctctttcatc atcttgggct
    14641 cgtattgatt tctaccgtca aatataatgg gttgacgaag agttgcttta atcttttcaa
    14701 aatcaggata acggaattga tgccactcag ttaaaagcaa cattgcatca gcatctttaa
    14761 gtgcatcata ttcatccgtg gcaaagtgta tcttgggttg agaaccgaaa atccgctttg
    14821 cctcattcat tgcagctgga tcataagctt gaatattagc tccagcatcc actaatgcat
    14881 ttataatagt aatagaagga gcttctctca tatcatcagt tttaggttta aaagataaac
    14941 cccagatagc aaaagtaagc ccttgaagat tatctccaaa atgactctta actttatcca
    15001 ccagaaccat tttctgttct ttattaatct cttctaccgc ttctaataat tgaggattca
    15061 gattattttc ttgaaacata tgaattagag ctttcacatc cttgggaaaa caggaaccgc
    15121 cataacccac acctggatat ataaatttat aaccaatgcg actatcggaa ccgatgccaa
    15181 tcctgacttc ggaaatatct gccccagtcg cttcacataa acgagaaagc tcattaataa
    15241 aggatatctt tgttgccagc atagcattag cagcatattt ggtcatttct gctgaacgcg
    15301 tgctcataat tatgatacgt tcattaacct tattgaaaga tagatatagt tctcgcaaaa
    15361 cttctgccgc acgaggattg tctgcaccaa taataattct atctgggcgc ataaaatctt
    15421 caatggctgc accttctttt aaaaactcag gattagatac cacatcaaaa tctatatcta
    15481 cacctctatc ttgcaatatt tccttaaccg tttttctaac taaatctgct gtgcctacag
    15541 gaacggtaga cttatctaca attatcttat aatcctgcat atagcgtgca atatctcttg
    15601 ccactgccaa cacatattga aggtctgctg aaccatcttc atcaggaggt gttcctacgg
    15661 caataaatat aatttgagaa gattgaactg cttcagcaat atcggtggta aagctcaacc
    15721 ttccgtcttt tgcattatgt tctacgagct cctgtaatcc aggttcatag ataggtacga
    15781 tattatttct tagcatttca atctttctcg gatcattatc aacgcatatt accctgtttc
    15841 ccatctccgc aaaacaggtg ccactaacca aacctacata tccactgcca accattgcta
    15901 tattcatttt aaagactcct tattcttaaa aaactctatg gtttctctaa tgccagcatc
    15961 aagagtataa agaggactcc agcctaattc tttaaaggct ttagagcaat ctaaaagtga
    16021 ccgtcgtaaa tctccctttc tgggaggacc tggtattggt ctgaggtccg tacctaattg
    16081 ccaggctata gttctgtaaa gaaatgaagt agtagtttcc tgtcctgttc caatattgaa
    16141 gacatcatta tctcctttat ccagtgctgc aacattagct ctaaccacat ctttaacata
    16201 tacataatct ctaatcatac cttctggttc atcaggataa gcataaagca taggagcttg
    16261 gttatggagc agtttttcta taaaaattga gaccactcca gcttcgcttt gtgctacctg
    16321 tcggggtcca tacacattgg cataacgcag tatggtatag gttaagttat attgatattg
    16381 ataaaagcgg acataatatt cgctggtcaa tttatgaata gcatatatgg ataaaggatt
    16441 caaaggacag ttttcagaag caggatattc ttccgcttcg ccatagatag ctccaccgga
    16501 agaaattaaa ataacctttc taacaccgta ctttacagaa cattggagaa catttaagaa
    16561 tccttttaaa ttgatttcag catcattaat gggattttca atggaataag gaacacttat
    16621 ctgggcagca tgatgattta cgacatcagg tttttctctg gcaaatatct cttccagagc
    16681 aggagaacag atatttaact gataaaattt cacccgtgaa tcaagatttt ctaaggaccc
    16741 ggatgaaagg tcatccacca ccactacctc atatccatca gccagatagg catcagcgac
    16801 attggagcct ataaatcctg ctccacctgt tattaatatc ttccttttca ttcttcttct
    16861 tctacaattt cataatcacc tataatttta atatcacgag gatcaacttt aaccgagacc
    16921 gcttgatgga gaatgttcac ttgaagatga acctcttcca attttttatg actttcaact
    16981 attccactca cacccttaag aggtccattt attacctcta ccatcagtcc ttctgaaagc
    17041 cacatagctt ctctcatctc tatcctacga ttacgagtta accatatatt tctcagttca
    17101 tccaccagtt ctttctgaga gggaacattt atgtaggaga caatataacc agaatagaga
    17161 agctctcttt tcgtgtagaa attaatgata gtaaagagat aaccgggaaa aataggttta
    17221 gtaaaagtca cttttcttct ttgatagata cgactgcttt cagtgagcgg taaatagtag
    17281 gatatattat gttttagggc atattcagcc aatttctttt cacacctcgg tttagtgtga
    17341 atcactgacc agaaatgatt ctcaggtgct aattctattt tcccaaaaag ctcttctatc
    17401 tcaactggtt tatgagtgga cataaagctt aatatctata atgctcaggc ttatagggtc
    17461 cggaaatggg aacatttata tattcagctt gctgacgtgt taaatgagtg agcacaacac
    17521 ctaatttatc cagatgtaat cttgctactt cctcatccag tatcttagga agagtataaa
    17581 ctccaatctc ataattattt tgccacaaat ctatttgcgc taagacttga ttggtaaagg
    17641 agcaactcat aacaaaggaa ggatgtccag tagcattacc aagattgact aatctgcctt
    17701 ctgaaagcag gataatatat tttccattag gtagataaat cttatccact tgaggtttga
    17761 tgttttcgcg cttgatgcct tctaattcat ataacttttg cacctgaatc tcattatcaa
    17821 aatggccaat attgcaaaca attgcacctt ctttcattcg gagaatatgg tctactctaa
    17881 ttacatcaca atttccggta gcagttacaa agatatctgc ataatccgcc atataatcca
    17941 ctgtattcac ttcaaatcct tccatagcag cttgtaaggc acaaattggg tctatttcgc
    18001 taaccaccac gcgagcacca aagccacgca tagacttggc acagcctttt cccacatcac
    18061 cataaccgca gaccattacc actttccctg caatcattat atcagttcca cgcttgattc
    18121 catctgccag agattctcta caaccataaa gattatcaaa cttggatttt gttaccgaat
    18181 cattgacatt aatagcagga aatagcaatt cctgatgagc gagtttctga tacaatctat
    18241 taactccggt ggtagtttct tcactcactc ccttcaatct tttaactata cgatgccatt
    18301 tctcaggatc agattgcaaa ccggaaatga gaagttcttg aactatcctc atttcataag
    18361 aatttgcatc aatctccgga agcttgcccg tagcataata ttgttcttcc agtctgtatc
    18421 cttcatgaac cataagagta gcatcaccac catcatcaac aatcaggtcg ggacctccat
    18481 cttgccaggt taaagcattc caggtgcatt gccagtaatc ttccagagtt tcccccttcc
    18541 aagcaaaaac aggaatacct cttgctgcta tagctgccgc tgcatggtcc tgagtagaaa
    18601 atatattgca acttgcccaa cgaacctgag ctccaagctc aatcagagtt tctattaata
    18661 ctgctgtctg gacagtcata tgcagacttc cagtaattcg tgcaccttgc aaaggttttg
    18721 tttcagaata tttagacctg atagccatca agcctggcat ttccgcttca gctaaaatta
    18781 tctctcttct gccaaaatca gcaagtgata tatctgctac tttatattcc attttagtct
    18841 ccataaaatc ctttatagtt tagagaagaa cgatatcatt tatctcttac gataatttgg
    18901 attattgtgt cctgaaggat gataacttcc tttcctgtca tcacgtctat tgggtctatc
    18961 ctgcttggtt gaattgtggt tgggttcagg attaccctct actcctttca tggttaagga
    19021 tagtttccct ttttccattc cgagagcttt aacatggaca atatcaccta atttaaccat
    19081 atcttctgga ctaccaatcc tggaggtatg catttgcgat atgtgtacca ttccctcttt
    19141 agttcctccc agaatcttta caaagattcc ataaggttca atccttgtaa cctgtccttc
    19201 aaattcttct cctatttctg gttccctaat aatgctttta atcaattctt ttgcttttcg
    19261 gatagattcc tgattgggag aaaggatatg cacccatcca tcatcttcta tattaatttc
    19321 cacctggcaa gcttcaatga tagatttaat catctttcca ttaggaccaa ttatttcacc
    19381 aattttatca actgggactt ggaaagcttc tatacgtggc gctgtaggtg ccagttcttt
    19441 tcttggttcc gcgatgcact cttgcattat atccagaatt tttaaacgag cattatgagc
    19501 tttatccaga gctattttca taatatcttt ggtgattccc tcaatcttaa tgtccatctg
    19561 catagcggtt ataccatcac gagtgcctgc acatttgaaa tccatatctc ctaagtggtc
    19621 ttccaatccc ataatatcgg ttaagaccac atagttttct ccttccataa ttaaaccatt
    19681 ggcaattcct gcgacaggag ctttaatggg gactcctgca gccattagag ccagacaacc
    19741 actacagatg gatgcttggg aagaggaacc atttgattcc agagtatctg taaccactct
    19801 cacagtatag ggaaaaattt ccttatctgg cataactgct attaaagcac gctctgccag
    19861 attgccatga cccaattctc tacgacctgg agctcccatt ttaccaactt ctcccacact
    19921 aaaaggagga aaattgtaat gaagatagaa tctcttctta tactccgttt ccaatccatc
    19981 aactatttgc tcatcgctac taccaccaag agtaactgtt cctaaagatt gagtttctcc
    20041 ccgggtaaat aaagcagaac cgtgaacaat cggcaataca tcaatctcac aggttacttc
    20101 ccgaatatcg tccaaacttc taccatcaac tctgtgatgc ttattgagga ttgaatcgcg
    20161 tacatagcgt ctaatcagct cttcataagc agctttatac catttttcat tctgagcata
    20221 ggtttcttca ttttctgctt ctgcctgata cttttccagc atttcagctt cggtggtatc
    20281 aaatgcatcc tgtctttcct gtttaccaaa taacatagct gcttcagcca ctctactgcc
    20341 gtaatcagct tctacctttg ccattatctc ttctggaata ctttgtaaga caacctctat
    20401 tttttcttta cctgcttcag caacaaagtc attctgtaac gcacagagtt ttttaatctc
    20461 ttcatgacct gcatatacag catccagcat cacttcttca ctcagttctt tagctgcaga
    20521 ttcaatcata accacagagg ttgcagaacc tgccactgcc aaattaagtt gagaaagatt
    20581 gtttagttca ttctgggaag ggtttatgat aaactgattc tcaatataac ctacggttac
    20641 gccagcaatg ggtccatgaa atgggatatc ggaaatcatt aaagctaagg aagctccaaa
    20701 aaccccaatt tcagaaggtt cattctcacc atcataggat aaaacattaa ccactacatg
    20761 gacgggatta cggaatccat ccgggaaaag aggtcttata ctcctatcta ttaatctggc
    20821 agcaagagtt gcatctgtag ttggtttagc ttcacgcttg aaaaaaccac ctgggatttt
    20881 tccactggca tacatttttt ctacataatc cacggttaaa ggaaagaaat cttgctcttc
    20941 agacggttct tttcccattg tagctgttac caagatagag gtgtcaccat acctaatgaa
    21001 gacgctaccg ttggcttgtt ttgccatcct gcccgtttca atgtaaagcc gacgaccaca
    21061 aaattctaat tcccttttaa taatattaaa gttattcatt aacttctcct ctttatcata
    21121 tccaagggag aagaagcaat aggcagaaag cggtttatat tctgctctca gcttattgct
    21181 caaactccct cggaatttat tgcctttatt ttccctttat aagattattt caagaaagaa
    21241 ggtgcccgca atgattacta tattgcggga acccacttcg taatataggg agagtttact
    21301 attttctaag atttagcgcc ttgattagtt ctctataacg agcaatatct ttcttcttta
    21361 aataatcaag taaacgcctt ctttgcccga tcagtttcag aagtccacga cgagtgctga
    21421 aatcctttgg atgctgcttc aaatgctcgg ttatatcatt tattctgcga gttagaagcg
    21481 caacctgtac ctctggtgaa cctgtgtctg attcatggag tctaaactct gccatgatag
    21541 cctttttggc ctcaggtgtt aaagtcattt catcctccag atgaatttta atacagactt
    21601 ttttagctac tttatttgtc cagcaatttt taataagtag aaggtcttat aataatacat
    21661 attatctgat aaagtcaaag aaagaatgaa aagaagcact ccaaaatggc aaacaaggaa
    21721 acaaaaatca ggggatacaa attaaaacca tacccccttt aattcatcca gataggtcta
    21781 taatcttaac atttcatttt ataacacaga ttttttgagt aagcttcttt cctctaactt
    21841 ccgtttgaat tagatagatg ccctgagcta cctgcttacc atttttatcc aaagtattcc
    21901 accttaattt actatcaggt tctaccatat cttcttttac cagttcactt ttgagattat
    21961 atactttaac cttatagctg gatttagcgc cctttaattc tatattaact aaatttgtgg
    22021 taggattagg ataaactcat agctgaacag ggatagacag tgggatgttt caatgcttct
    22081 ttttctttag tataaagaaa tggcacaaaa tggtcttctt taaaggaaca tagccatcct
    22141 tagtgatgat aatgtttatt ttccgctctg gaatcccttc ttgtaaaacc atatattcat
    22201 ccaacataac tggatagaca ggacttgggg ttttataaag atagaggtca atatcataga
    22261 gcgaaacaac tgcacctgag ggtttagtta taatttaaaa gcannnnnnn nnnnnnnnnn
    22321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22381 nnnnnnnnnn nnnnnnnnnn nnnttagtct ctgaattaat gaacatatat tcattaatta
    22441 gaggattgaa tgttagctaa gtgtaagtgt aaaaacatat ctaaggcaaa attgacagaa
    22501 atcatagatg gccttcctga agagaacatt atagaccagc tttgcgaatt cttcagggtt
    22561 tttggagaca gcaccagact gagaatattg tattttcttt cttgtgatga attctgcgta
    22621 gctgatctat caaatttagt gggaatgcaa caatctgcag tttcacatca acttcagact
    22681 ttgagacttc atcgtctggt aaaatgtcgt aaagaaggga ctacatctta ctattctctg
    22741 gatgatagtc atgttcaaga aatttttgga atagctctga agcacattaa tgaagtttta
    22801 gtaaaatgac taccaaagag tatgatatag agaatttgca ttgtgcagaa tgtggcagca
    22861 ggattgaagc cggaatagag aagttacctg aagtggaaag tgctaatctt gattatcaga
    22921 ataaaaggct ttctataagg tatcacaaag cggttgatga ccctttagcc cgtctttatc
    22981 aagttgctgc ttccattgat cctggagtgg tcttcactct tcatccggtt agtatgatca
    23041 ataagctatc ttccttattc tggatatttt tagtgatagg tgtaggattg ctaatagcta
    23101 cagaatttgt tccttcaact tatcaacctt ggttaggaat actgggttgg tttttagtag
    23161 gatatagggt tctggcacaa gcgttaaaat ctctcacgca aaaaaaggta tttactgaac
    23221 atttcctgat gagtttagcc accattggag ctcttatttt aggtgaatat cttgaagctg
    23281 gcgcagtgat gattttctat gagtttggcg aatggttaga agataaagca ctggaacatt
    23341 cccgtcaaat ggtaaaaaat aaacttaata taaagactga aattgctcat ttacgaacgg
    23401 aagatgatat tcaggataaa cctttatcta ctatatcaac tggtgatgtt attgtcatct
    23461 atcctggtga aagagttcct cttgatggca ccattataaa aggtgaaagc accgtggata
    23521 cctcttcttt aactggagaa agtgaacctc tttatgtagg tgagggaacc gagatttatg
    23581 ccggtttttt aaataattct ggaatgctgg aacttcaggt aaagagtgtt gcctctgaga
    23641 gcacgataag tcgtatcctg aaattgatag aaaatgcagg agcagccaaa tcgcctcaag
    23701 aaaaatttat tacccgcttt gccagaattt acactcctgc tgttgtagcg gcagcatttt
    23761 tagtattcct tataccaact ttattaggtg ctgaagctgc aatctggttt aagcgttcat
    23821 tggtatttct aattgtttcc tgtccttgtg ctctggttat ttctattcct ttaacttttt
    23881 ttatcagcat tggtattgct gccaaaaaag gaataatagt caagggaagt ggttatctgg
    23941 atatattaag acaggtacga accatagttt ttgacaagac aggaactctc actactggtg
    24001 aaatgcagat tgataaagta tatgtgaatg gagatattaa tccagaagtt ttattagaag
    24061 ttctctggct ttgcgaatac aattcatctc atcccttttc tcgggcaatt aaaaaagcct
    24121 atcagggaag ttttgatagt aagcaggtta aagcctattc tgaatttccc ggaaaaggat
    24181 taattcttat ttatggtcaa gacagattgg tggcagggtc tgatgatttc tttgctaata
    24241 tgggatttct ggatttgatt aatccagcta atcaaagtgt ggtacactta gccaaaaatg
    24301 gattttatct cggttgtata accttttccg atgaagttaa accaggaatg aaagaggcaa
    24361 tagaaaagct gaaaaaacaa ggaataaaga agacggtgat gctttctgga gacagagctc
    24421 ctaaagctga aaaagtagca aaggaacttg gaatagacga gttttacgcc gaactgcttc
    24481 cggaagagaa actggctaaa ctggaagata taattaataa aaatgaggga aaaaccgcct
    24541 ttgtaggtga cggactgaat gatgctcctg cacttgctcg tgcagatgtg ggaattgcta
    24601 tgggtggaat tggcaatcaa gcatccattg aaagtgctga tattgtccta ttaaatgacc
    24661 gtcctcatca attggtagaa gcatttacta tatcacgcca aacaggcaat atggtaatac
    24721 aaaacatcgt tcttgcttta ggaataaaag cattgattat gcttctgggg attttaggaa
    24781 tagctggact ctgggaagca gttttaggag atgtgggtgt cactatttta gtgatattca
    24841 attccttgcg attgctaaga tacaaagccg atacttgaca aaaacatttc tttgtgatta
    24901 atggattaaa taaggtaata atcaggagtt ggaatgcaga taaaaatcac cttgccagac
    24961 ggttcagaaa aaatatatga ccatcctgta actgcaatac aggtagccaa agatttaagc
    25021 ccccttttag ctgaagaagc gatttgtgcg gaagtagatg ggaaactcgt agatttggat
    25081 tatctgattg accataatgc acggttaacc atccatactt ttaaaacaag agagggaaaa
    25141 gaagtttact ggcatagcac cgctcattta atggctcaag ccgttaagca gctatttcct
    25201 caagtcaagg tggcaattgg tccagccata gaacagggct tctattatga ttttgataag
    25261 gatgaaccct ttaccgatga tgagcttcaa cagattgaag aaagaatgat tatgctcagt
    25321 aaagaaaatc ttccttatac aagacaggaa ttaagcaaag aagaagctaa aaaaatattc
    25381 agtgaattag gtgaaagtta taaattagaa ctactggaag atatacccga cactgatacc
    25441 attagcgttt acagacaggg tgattttata gacctgtgta gaggtccaca tctacctcat
    25501 actggaaaaa taaaattcat aaaactcctt aaatcttccg gtgcttactg gcatggtgat
    25561 gaaaaaaata agatgcttca gcgaatctat ggtatcagtt tcccaactgc caaagaactg
    25621 gatgattatc tggaatttct taaacaagca gctcttagag accaccgtaa attaggaaag
    25681 gacctggacc ttttttctat aaatgaagag gtcggacctg gtttggttct ctggcatccc
    25741 aatggtgcta tgattagaca tctcattgag tcttactgga aagaacaaca cctgctaaat
    25801 ggctataaat ttgtttacac acctcatatc ggaaaagcca gtttatggga aaccagtgga
    25861 catctcggtt tttataaaga gaatatgtat tcagcaatgg atattgaagg gcaggattat
    25921 tatattaaac caatgaattg tcctttccat atcgctattt acaacactaa tctacatagt
    25981 tatagagagc ttcccatccg tcttgcggaa ctgggaactg tctatcgtta tgaaaggtct
    26041 ggtgttttgc atggtctgat gagagttcgt ggatttacac aggatgatgc tcatatcatt
    26101 tgtactcctg aacaacttaa tgaagaagta gaaaaactca tcgtctttgc tcttgatatg
    26161 ctcaagcatt ttggatttaa agagtttttg atatatctta gcactcgtcc agaagagtgt
    26221 gttggagatt ttactgattg gtgtactgca acggaaagct tgcgccaatc tctggataaa
    26281 cttggattgg aatatgaagt agatgaaggt ggtggtgctt tttatggtcc taagattgat
    26341 ataaaaatcc gtgatgctct tggaagagcc tggcaatgta ctaccatcca gtttgatttc
    26401 aatgaaccta cccgctttga tatgacctat attggtgcgg ataatgctcc tcatcgtcct
    26461 tttatgattc atcgtgcaat tctgggttct atagaacgat tttttgccac tttactggaa
    26521 tatcattcgg gaaatctacc tctatggtta tcacctatac aattaataat tctacccatt
    26581 accgataacc aactggatta tgcaaataaa ctaaaggaac aatttcttca aaaaggcatt
    26641 cgctgtgaag tggatgcaag aaatgaaaag ataggttata aaatccggga agcagaaaca
    26701 aaaaagattc cctatatgtg cattgttgga gctaaagaat tagaaaacaa catcttaact
    26761 ttgcgtcgtc atctattggg tgatttgggt gcaatgacct ttgatgaagc tctcaatcgt
    26821 ttcagagaag agctctaatc ttactctatt ttgttcagga aaaatagatt atgtctactc
    26881 ttgaccttat ccgtgaggaa ttaaaccacg aagcagaagc catcaaaaaa gtagctgaac
    26941 agcttaatcc agaagaagtg gataaagcgt ttaatttgct aagaaattgt aaaggcaagg
    27001 tagtgcttac cggtataggg aaatctggaa ttatagcgag aaaaatttct gctactcttg
    27061 ccagtacagg cactacttcc atatttttac atgctgcaga aggagtgcat ggtgaccttg
    27121 gaatgttgca gcctgatgat gtggttatag ctgtttccaa tagtggcaac actcaggaat
    27181 tactttctat tattccttac attaaatata ttggtattcc cttaattgcc attactggca
    27241 atccacattc acctttagca gagaccgcgg atgcagttct ggattgcagt atccctcctg
    27301 aatatgaagc tttagggatg gtgcctactt caagcactac cgcagaactt gccttaggag
    27361 atgcactggc aatcctgtta cttaaggata agggattctc actggaggat tttgcgcgct
    27421 ttcatcctgg aggcaatatc ggaagaaaat tgcttatcaa agttgatgaa cttatgcata
    27481 ccggagatga attacctcta attggagaag aagccgctat gagtgatgcc atactggaaa
    27541 tgacctccaa aaaacttgga tgcgtagctg ttgttgataa agataagcat ttaactggca
    27601 ttatcactga tggtgacctt cgtcgtcaaa ttcagcaaaa acaggataac ttactgcaac
    27661 ataaagttaa agagtgtatg actccaaatc ctaagtgtgg ttatccagat cagcttgctg
    27721 cagaagcatt aaaactgatg gaagaccata agattactat gctacctatt gtagataata
    27781 ctaattctct ggtcggaatg ctacatatgc atgaccttat agaagctgga atattataac
    27841 tttactctac tgtcacactt tttgccagat tacgcggctg gtctacatcc actcctttta
    27901 aatctgctac ataataagct aataattgta gtggtatcac agtaagcaga ggttgtaaat
    27961 cctgtaaagt tgaaggcaga taaataacac tttcggaaat cttttctaaa tctttatttc
    28021 cttcgttggt tatagtaatt aagcgagctt tacgagaatg cacttcttcc aaattattat
    28081 gaatcttatc atataaaggg tcattagttg caatagcaac aacgggcatt tcttcatcaa
    28141 ccagagcgat aggtccgtgt ttcatttctg ccgctgcata accttctgca tgaatatagg
    28201 atatttcttt aagtttcaag gctccttcca gtgctacagg ataatttata cctctaccca
    28261 gatacatagc attgcgtgca tccttgatag tttctgcaat ctgacggata tattcacttt
    28321 gctttaggat actatcaatt ttagaaggga tggtttgtaa ttcacggatg tattccatac
    28381 cttgaagaga ggaaagattt ctctgtcttc caagtaaaat tgccagtaag aagagaatag
    28441 taacctgaga agtaaaggct tttgtggaag caactccaat ttcacttcca gcatggatat
    28501 acgaacctcc attactttct cgggctacgg aactacccac cacattggtg ataccaaaga
    28561 ccttagctcc tttggattgt gcttctctta aggcagcaag agtatctgct gtttctcctg
    28621 attgacttat aacgaatatc aaagtatgct cctgaataat aggattgcga tagcgatatt
    28681 cagaagcata ttccacctgt acaggaattc tcgctaaatc ttctatcata tacttcccta
    28741 taagagcagc atgataggaa gttccacacc caaccagatg aatttgttca attttgctta
    28801 tatctttagg tgtaagcttt aatccaccta atctggcatt accactggtt tcatcaattc
    28861 ttccacggaa agcattttcc accgtcgttg gttgttcaaa gatttcttta agcataaagt
    28921 gcttataagg tcccttatca atggctgaaa tatcccaatc aacagtagaa acttccggtt
    28981 caatcttttc tctaagacca ttggtgatat caaaatgatc tttttgcaga atgcataatt
    29041 catcatcttg aagataaaaa actcttttgg tgtgaattac aatggcagaa acatcactgg
    29101 atataaaatg ttcgttttct cctattccaa caatgagagg agagcccttt ctgattgcca
    29161 gtattttatc tggttcatct tcacataaaa ttacaagtcc ataggtgcct tcaactttgg
    29221 ataaagcagc tctaatggca tcttccagag taggttccga ttttctatat tgctcaatca
    29281 gatgcgctat aacttctgta tcagtctcac tacggaaagt gtgtcccagt tctatcagtt
    29341 gcttttttaa aggattataa ttttcaatga ttccattatg cacaatcgct attttgccgt
    29401 gacaatcaat ttgaggatga gcattaactt cactgggttc tccatgagtt gcccatctgg
    29461 tgtgagctat agcaacatta ccagaacatt gatttggctc gggtaaagag cgttccaggt
    29521 ctacaatctt accctgtttc ttgtaaattt gaagtttgtt attatgaata atggcacaac
    29581 cagcactatc acatccaaga tattccagac gttttaaagc ttcaataact atgggcaaag
    29641 cattcctttg tccaatatac ccaacaatat tgctcataat tgaacctcct ttgagacggt
    29701 tattttatta aataccttca ttgatataat aaatataaag atggctggaa gaataaccca
    29761 gtattttaaa ttttcaccat cctgaataaa aatcttcatc caattctgtt caccaaaggt
    29821 agtaatcact actgccagag tgttattgat tatatgaaag aacatactat ttacgataga
    29881 acctgtccgc atagctaaat atcccaagag gattcctaaa agcagcaccg ggagaaagcg
    29941 gaaagggtct aaatgaaaaa cagcaaaaag aatagctgta gtgattatag gataccaaaa
    30001 atgcttttgc tcaaagaaac gcatcagaaa gccacgaaaa agtatctctt cacatattcc
    30061 aggaatcagt gctattacta taaacaattc ccagaatgag atatcctgcg taaagatacg
    30121 agacatagct tcaatatatt cttgtgggaa aggaaagata tgatttatca cttgagcaat
    30181 aatgctcact atcgtggaag cagatatagc aatgaaagga attattgcta gttccttagc
    30241 tttaggtggg ttaagtcgca atatttcagc aggtttgtat tttcccagtc gtagaactat
    30301 taaaactgga aataagatta aaatcagttc ggattgtatc aacccattga ctaaatctgc
    30361 tgtttgccat tttcctccaa gataatagag agctaccagc gcaaggcaga aatacactat
    30421 cccataaaat ggagagaaga aggtgcgttt attctttatt ttaattttac caccttcttc
    30481 ctgtgtgcgg aagagaactg attcagtatt gaataatttc accgttatcc agatggcaat
    30541 cacatccaga acgagagttg aaccaatagt caaaagcagg tgtgaaagtt gataattccc
    30601 aattaataca gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntccaaatc
    30721 caatcctgtt tgagaaagca tatattgaat ggaaaaactt atactaacca gattgattac
    30781 aacattaacc attgccatag taacaatagt gagatatttc cccagaacaa tttccccacg
    30841 agaaacagaa gagaccagca aagtctccag agtttgtcgt tctttttctc ctgcaacgag
    30901 gtctgctgcc accacagaag caccagttat tagaagaagt atcattaagt agggaagcat
    30961 agctcccaga acacttccca tctttttctc agcagtggat gtatctatcg gtttaatttc
    31021 cagaggttta agggtctgct ggtctatatg caaagcttct aatctttgag cagtaatctt
    31081 ttgtgaggtc tccaataatc tattttccag tttatgatag accatcatac ctttttctcc
    31141 agagccatca tagcgaatag atactattaa gacaggctct tgcaagacat tcagggaatc
    31201 tttaacggtc acaatagcat caatttcacc ttcttgataa agcttttcca gcgctggtgg
    31261 tgcaggaata atgctgaaat tttctatttt cttcaagtct tcatagattg aatcccggat
    31321 tgctaaagtt tcaggagtac gaaaagatag gctatcctga aaagctatag tagcacctct
    31381 cttttccaga acatcaactt ggcgactcat aatcgcactg aaaccaacaa aaagcaaggg
    31441 atagagaatt actggtaaaa ttatagtggt aaacagggtg cgtttatctc taaacagttc
    31501 cagcatttct ttcttatata tgacaatcgc ttttttaaaa ttcattcgca gctaccttca
    31561 attctccatt ggcttgattg atgtaataga taaagatatc atcaaggtca gaaagattag
    31621 tctcttccct catttctact aatgaaccct gttttagaac tctgccctta tgtatcatta
    31681 caattctatc agctaatctt tcggcttcgc gcattatgtg cgtggaaaaa agaacacatt
    31741 taccctgatt cctacaatct ctgatgaatg aaactatatt tctggaagtt agaatatcca
    31801 atcctgctgt gggttcatca aatatcatca ccggtggatt atgaatgata gaacgtgcaa
    31861 tggaaacctt ttgtttcata ccactggaaa gaaattctat ctttttatcc agaaattcat
    31921 tcatatccag aatctcagac atctctctga ttctatgttc tatcattcct tcaggaactc
    31981 catagagtct accaaaataa gtgataaatt cccggggagt taacctttga tataaaccag
    32041 tatcaccaga gagaaaacct aaattttctc taactttatc tgcttcagtt acaacatcat
    32101 atccttcaac ttcagctttt cctgaatcag gtttaaatat ggtagaaagt atgcgcatag
    32161 tagtagtttt gcccgctcca ttaactccta aaagacagac tatctcatta tttgaggcat
    32221 caaatgtgac ctcatctaca gcacgaaaac tcgttccatc cttttgtatg aattccttta
    32281 ccaaagactg gacttttatc attagtatcc ttttaccata cttttttatt agtgttaaag
    32341 aaattagcat acactactaa tgtcaagttt ttctcatata tcttattttc ctcttctcta
    32401 aaaatctaag ataaatagta tacagagata atgaaaatag acaaatagtt agattaattt
    32461 attgattatc ataatattat gagataatag ataatctttc cagttcatta tttattattg
    32521 aaaacaatgt tcctgttcat atattaataa ttttgcttat cttgtatctt acctctaaaa
    32581 agataaccac cttatgtcca gcttatgtcc agcttatgtc cagctcatgt ccgaaatcgg
    32641 tcataagtta tacttttata aaagataaag tatccatctg taaacaaaga agaaaaatcc
    32701 acacaaaaac caaagctgga ttttataata ataaatgaat ctaagggcaa caagtattgg
    32761 atattttcct aagaggatgg acttttaatt tattagaaat aaatctgttt gtgcataaag
    32821 aagtctggaa cttatttttt ctgttataat tttgaattgc agtcaataat aagaacaaaa
    32881 atagaaggaa aagcttgact aaaaagtata catataaaaa ggtagtaata gtaaatatag
    32941 agcgctgtaa ggaccagcaa aaatagataa agcgagataa tcaatcatcc catcactata
    33001 ggaagtgagg aatatccatt atgagagacc cattcagtat acttaacacc ctggaagctg
    33061 cttatgtttt ggatgtagct acaggaagag gtgacttcat taacatatta agagacaatc
    33121 taaaatctta tgttaagata ataggtgtag atgcatcgga caaaaatgtt gaccgtgccc
    33181 aaaagctttt ccccgaaaat gatatagaaa tatatcagat gaatctggga gcattacgtt
    33241 tggatgacaa tagttttgat ctggtaacta tcgcagattc cttgcatcac cttgacaatc
    33301 tggaagcagt attttctgaa atgatgcgtg tattaaaacc aggtggttct ttattagtga
    33361 tagaaatgta tagtgatgga gaccaaactg aagcacaaaa aactcatatt atgcttcata
    33421 actggttagc agcaattgat cgtaagatgg gtgtatttca taataatatt tatccccggg
    33481 aagagattct ttctttagtg aacaaacttg gattagatga tttgattatt gatgattttt
    33541 actacccggt agacaatcct aaagatacca gaaattgcga atctttaagg aaaacctgtc
    33601 agaaatcctt taaacgtttg gaatccttag aagacaatga agagcttata gctgaaggaa
    33661 attccttatt agatcgtatc aataggatag gatgtgcctc tgcaagtaaa ctacttataa
    33721 tggggaaaaa accccttagc aataaaaaag aaaatcacaa ggagaaataa atgcctatag
    33781 aagaaaatct atttgaaatg tcgcaattac aacttacacg tttagctaaa aaattagaaa
    33841 ttcctggtta cagtcaatta aaaggtaaag acctggtgtt taaaatatta gaatttcaag
    33901 cagcacagga aggtcttgct tttgttaccg gatgtttaga gataactgat gatggctttg
    33961 gttttctgcg tttcccagaa aacaactatc ttcaaggtaa agatgatgtc tatgtgtctc
    34021 agactcagat acgaagattt ggtttgaaaa caggtcatat ggtttccgga ccagttcgtt
    34081 cccccaagga aggtgaaaaa tattatgcac ttctaagagt tgaagcagta aatggtatgt
    34141 cccctgattc tgtccaaaat ctacgttcct gggacgaact taccccctac tatccaactg
    34201 aacgcttgaa cctggaattt gacccacata actattcaac tcgtatcata aatcttttta
    34261 ctcctattgg aaaaggacaa aggggcttaa ttgtcgctgc acctcgtaca ggaaagacca
    34321 ctttattaca aaatactgct aatgctattt tgaccaatca tcccgaggtt catttaattg
    34381 tgttattggt tgatgaacgt cctgaagaag ttactgaaat gaaaaagatt cttaaaccag
    34441 gaaatcgtga agtgataagc tctacctttg atgaatcacc caaaaaccat gctgcagtaa
    34501 gtgaaatggt tctggaaaaa gctaaaagaa tgattgaatt gggtcaggat gttgttatag
    34561 tattagatag tatcactcgt ctggctcgtg cttataataa cataactccc tccagcggaa
    34621 aagttcttag tggtggaatt gatgctaatg gattgataaa accaaaaaaa ttcttcggtg
    34681 cagccagaaa tacagaagaa gctggttctt tgactataat tgccactgca ctaatagata
    34741 ctggttccaa aatggaccag gttatctttg aagagtttaa aggtacaggt aatatggaac
    34801 tggtgctgga tagaactatt tctgatatgc gactttatcc tgctattgat ttggtaaaaa
    34861 gtggaaccag aagagaagaa ctgctcctaa ctaaaaatga attgaatcgg atgtatgtgc
    34921 tgcgtcaata tttaaagaca ttaagtcctg tccaagcgat tgaaacttta cgtaagaaga
    34981 tgcaagccac cagtaccaat gcagaactat tgaactctat gagcaattaa aatagataat
    35041 tgtgatgttc tatgccctat aaactaaggg tatttctact aactgcctta ctaattgctt
    35101 tgcccttaat gggatactca gcaggattaa gcaaagattt ccagattctg gaaaataagt
    35161 ttcagcaagg tgatattgct ggaatatctt ctctactccc cactgccaaa gctaataatg
    35221 atgaagatag ggcactacaa ctatatcttt cagcaatgct gaaaaagaca gagaatgaaa
    35281 ccatctcctc actacaacaa gcagcaaatt tatatcccaa aacctattat ggacagatga
    35341 gcatactgga actggctaaa tatcacattc tgaaaagaga aataaatcaa gctgaagaac
    35401 ttttacataa aattaccaat gttcaactca tagaaaaatt ctattggtta gcatattgtg
    35461 ctgattcaaa gagtaattac gataaggcaa tatcctatag tgaaaattac cttagaatga
    35521 catcagataa taaatatttg gaaaatactt actacataat tgcaaatgca tatagaaaac
    35581 agggtaaata tcaaagtgca atcactaccc tggaaaagtt aaaaaagata gaaggctatc
    35641 ctaaaaatga gcaatatttc tattatcaat tagggtattg ttatcaactt ttgggaaatg
    35701 tagccgatgc ctatcaaaac tataaaaaag cctttgaatt gaatcataat tcacctctgg
    35761 catttatggc agaagacaaa ctatttgagc ttcgtaatcg ttataatcca tccttagatc
    35821 tatctttcct ttatccttat acaactctgg atattccagg actaatccaa ccagaaataa
    35881 caattcctcc agcggcttca ataatacagg atttgccact taaactggaa aatagacctg
    35941 aaacaggtta ttatgttcag ataggttgtt ttagttctga agccaacgct cgtaaaagaa
    36001 cagaagaaat tcgtggtctc aatatctctg ctatatattt tgaggataag aataagaaga
    36061 atactccctg ggttgttgcc agcggtcctt attcttccct aacggaagca gaaactttaa
    36121 aaagtattct ggctcaaaaa tctgtggata gttttatcac cagatactaa taaagatgca
    36181 gactccttcc ccatctaaga caaaactcac cccaatgatt gttcaatatc aaaagataaa
    36241 gaatgaacat ccggataaac ttatcctttt ccggatgggt gatttctatg agactttttt
    36301 tgaagatgct tatactgcat caaaaatatt aaatattact ctcacttcac gagataaaag
    36361 tgatgaaaat cctgttccac tggctggttt tccttatcat gctctggata actacctgga
    36421 taaactgatt aaagcaggaa ttaaagtggc tatctgtgaa cagatggaag accctaaaca
    36481 ggcaaaagga ttggtgaaac gagaagttgt agagataatt actcctggaa cggttttaga
    36541 ccataactta cttggtggta cagcaaataa ctttctttcc gctatctact atgaagatga
    36601 aaatcgtaaa ataggtttag ccagtttaga cctttctacg ggagaattta tctttacaga
    36661 attaagcaag aatgagctgg caaatgaatt gctaaaattg aaacccagtg aaataattgt
    36721 taatggttcg gactcagaag cttttatcaa aaatttaaat ctggaatatg aaccaactat
    36781 cactatattt gataactggc agttcagacc ttccgaagca gccaatgtct taaagaaaca
    36841 ttttcaggtt cattctctgg aagctttcgg tgcactggga aaaaatatgg gagttactgc
    36901 tgctggagct gctcttgcct acattcaatc tctcacctcg gatcccttat ctcatataag
    36961 ctcacttcgt ttctattccc tggataactt tatgctgttg gatgaaatca cccgcaggaa
    37021 tctggaattg acccattctt taagatacaa tgataggcat ggaagtttac tctatgttct
    37081 tgatcagact caaacaccaa tgggctctcg tttattacaa aattggctac ttcatccctt
    37141 gctggataag gaacaaatta taaaaagaca ggatttaatt aaagcttttt tgaataatac
    37201 tgcctacttg aaagatgtaa gaactatact acataatatc ggtgatattt cacgtttagt
    37261 atccaaactt ggtgctctgc gtattaaccc aagagagcta cttgctctca attcctattt
    37321 attaaccgtt gccgacctca aaaaaaagct aatagcattc aatcatcccc ttctggataa
    37381 ttttattcct gctctggaag gatttgagga aataattgaa ataatcagta aagctattcg
    37441 tcctcaacct cctatcacca taactgaagg tggaatcttc aatcagggct ataatccaga
    37501 tttagatgaa ttactggaaa tcattcatga tggaaaatcc tggattgcaa gattggaaga
    37561 agatgaacgt aataaaacag gaatttccaa tctaaaagtc ggttataata aagtctttgg
    37621 ttactatatt gaagttactt cttcccagaa aagcaaagtg ccagattact atattcccaa
    37681 acaaactctg gtcaattctg aacgctatat atcccctcgt ctgaaagaat ttgaagccaa
    37741 agtactcagt tctgaagaaa aagtaaaaaa tctggaatat gaactattta aacaattacg
    37801 tcaacagctt gccggttatc tggagaaatt acaaagttta agtgagacta ttgccttaat
    37861 agatgtgcaa agctgtcttg cctggttagc ctggcaaaat ggctattgtt gccctgaatt
    37921 tactgagcaa agaactctga aaattgttga aggtagacat cccgtaatag aaaagctact
    37981 cactgatact gattttattc ctaacgacac agaattggat tatcctcaga ctactattgc
    38041 tataattact ggacctaata tggcgggaaa atcaacctat ctgcgccaag ttggattact
    38101 ggtaattatg gcacaaatgg gaagttttgt tccggcaaaa caactccaga tgcctatatt
    38161 tgatagggta tttaccagag tgggagcttc cgataattta gctcaaggac agagcacatt
    38221 tctggtagaa atgattgaaa cagcgaatat tttgaatact gccacacctg actctcttat
    38281 tttactggat gaaataggaa gaggcacttc tacatttgat ggtttaagtt tagcctgggc
    38341 tattattgaa tacattgaaa aatataaaca ttccctcact ctttttgcta ctcattacca
    38401 tgaactaact gaactggaaa gtatctatcc cgatattcgc aattacaata ttgccgtccg
    38461 tgagtataat gaacagataa tatttttgag aaagattgaa agaggcggtg ctgatcaaag
    38521 ttatggtatt caggttgcac gtttagcagg aataccggaa aaagttattc gtagagctaa
    38581 agaaatcctg aagaatttgg aagaacatga aatatcacct cagggactgg catcatcttt
    38641 acgcaaaaag cttcaagaaa cttcacctca gatggaatta tttgaggttc tggcagaaaa
    38701 agcggatgag cttgacccta ttataaatga gattaaagac ttggatctgg actctctaac
    38761 ccccatccaa gcctggcaaa aattaaaaga attacaggat aaattatgaa aaagcatctt
    38821 tggaaaatta tccccctcat tgttttcgct ttgttcgcca tttctattaa tggtgcacaa
    38881 aataatcctg aattagctgg tgtaataaat ggaacaccta tccgttatga tgaatttatt
    38941 caggaactac tcttaaatta ttataacttc cagtatgaaa ataaccgtgc tcctgatagt
    39001 agagaaaaag aattgattag acaacagacc tgggaagaaa aaactatgta tgtaatcctc
    39061 aaggactact ataaaaaata caatattaaa gtaacagctg aagagaccat agagtattta
    39121 aagagtaatc cacctgaaac acttaagaaa tctccacttt ttatgagtga tggcaaattt
    39181 gaccaaaaaa tatatgttca atctcttctt tatgatacac catataacct taaaccctac
    39241 agagaccggt atcagaatta tctaataccc aatcttatac tacaagacta tataatccga
    39301 gatgaactcc taacttcatc agaaaagaaa cttatcacga aaatacttca gagcagtgca
    39361 aatataaaat ggacgatttt agatactgaa agcataaatc cttatatcac tgaagaagaa
    39421 attcagatat actatcagaa acatctttct gatttcaaac taaagcctta tattaatctt
    39481 gcctatacca cgattagcgt ggttccctct aagacagata ttagattgac caatgctata
    39541 gctgattcta tatacaatga attaatgcga ggaaaaactg ttcaggacat attagaaagt
    39601 gagttaccca ttgctcctca tctaagtttc atcagtatgg attatgtgag agaaagtgaa
    39661 cttgaacctt caatttatac tatattaagc cagctaaaag aaggagaatt cagtcctcct
    39721 atagcagata aagaaggact caatatctat caattagaaa gccgcaccaa aagtatgtgt
    39781 agttataatg tcctaagaat accttatatt ccatctcaat ccagtataga tgtggttctt
    39841 ccaactgctc aacaggttgt aaaacttgcc cgtaaagaaa atctatctat tgcctgcgat
    39901 gagatgaacc ttgattatca aaagacaggc attatttctc ctgattatga ttggtttgga
    39961 gattctcagg tggtaaataa tatcttgaat ttaataccca actattccga tagatatgtc
    40021 tttgacccca tctattctcc caagaaaaag gtctgggtcg tggtagaatt gctggaatct
    40081 tcactttatt catatcaatc cctggatgaa gtgagaccac aaattaagaa aaaactggaa
    40141 gaacaaaaga gagctgaatt agccttgcaa ctggcagata aaattagctc aggacaatct
    40201 actatacctt ctgatgctcc cactatttat attgaaaata tgactatcaa tactccctta
    40261 cttgacaatg atgctgcaaa tatctattat cagacccttc gctgttattt taaaaaggag
    40321 aaacctaaag cctttatcat agatgaaaag attgtattac ctgaagtaat ttcagttaag
    40381 atagataaga agaaaaaaat ctcccaagac cagattatac aggtatacaa gaataacctg
    40441 ccaccagatt ggtttaataa ttggatggca tctaaggtta agaaggcaaa agttaaagtt
    40501 tacattcaat gaacttttac cttcttaggt tttgaatttt agagagctat ttcattaaaa
    40561 tagctctacg aactgagttg aattctttag attctatccg aataaaatag attcctgatg
    40621 atacacttcg gttctgtttg tcctttccat tccagacaat agtatgagtg ccttctctca
    40681 aataatcatc taccagacaa ttaaccagtt gtccttttat attgtatata ctcaatttta
    40741 ctggtccacc ttttgctaaa tcaaacctta tagtagtaga agggttaaaa ggattaggat
    40801 aattctgata tagcttggat accagaggat ttatattctg ctccgtaata tccacataag
    40861 gaaagctgaa acttatcggt tcagttccca cactttctcc cgtttcataa caggttatca
    40921 cataatagcg ataatttcca atggtgctaa gttgttcgtt ataataagtt tgagaatata
    40981 ttcccataag ttcataattt cctgcattaa gcttacgaaa tactttataa ttcaagatgg
    41041 gataaaacgg ctcttctgga gcagaccagt ttagatataa tgaatcctga actaccctaa
    41101 agcttaaatt tgtagctgga gcaaaataag ctaaaaagaa gtcgtgatta attgcaggat
    41161 tttgcaagtt aatattaatt gaagataagg tctcggtttg atatcctggt gcgatagcat
    41221 ttacactttg caagccccca ggaaggtaca aaacataatt cccatcggaa tctggttgtg
    41281 aagttaacag ggtggaattt tgaattaaaa cttgagaata gttaatttca ggattggaag
    41341 agaaaactat gccatataat ttaccagcaa agggaatata tcctgtggtt tgtacatcat
    41401 caatatacca gccccaaccc tgaatgcctg gatcagaaga aaaagtccag cgaaacataa
    41461 cattctgatt ggcataatta ctgagattaa atctggctaa tacccatccc tgactattac
    41521 cagtgtaacc tggaccacta agagcagaaa cattttgata tggataaccc ccttctggag
    41581 ttaaaagagt ccaagaaccg cctgaattgg tggatatttt taaattccct ccatcatatc
    41641 cctgttccat atcataccag tgccagaact caagcacaaa attgggtcct atatatatta
    41701 ccggagaggt taaagtccag gttgcataat tgggatattg ctgattcaat aaagttcccc
    41761 agcactttgt tcctgaatga gcacctacga ttgaatcaga accccattgc caggcattat
    41821 ttgccggttg agtgacaaat gaaccattat tattctcaaa atcagcatac atcccggtag
    41881 taccaacatt taaacggata gtctcattat gcgcttctat ggcttcaccc agataagtta
    41941 tatagaaagt gagattatta cctaccgata cattaggtga aagagtgatt tcatagataa
    42001 cctgacaggt tgcacctgct ggtatataat taatcaattg ctgaggattt aaaatatcca
    42061 catctgaact catacaactg atattgctgg tgatattata agctgcaaca cttccatcat
    42121 tggtgtagtg cacaattaaa ttaaaggtct cagctggatc tgccattcca ttaatattgc
    42181 cctgcaggtc atttatatag accccgctca aacttaattg aggttttttt accgttatgt
    42241 tttgaatgta gttccagcta ttgccactaa tactgaccgt aagatttaat tcaatagtcg
    42301 tattatgagg acattctgga ctaatagata tagttatcgg gtctatattc acggcagaac
    42361 cacttcctgg tatatcggga taatcacttt cggcattttg aattaagacc tggggattaa
    42421 cagtatttgc tatgatatgc acatcagttg cagtagctat tcccaagttg ttcaatttta
    42481 taccaatttc tccactttct ccaggttcca tccagccatt tccattatca tcctgcaaaa
    42541 taaaatcccc aataaccagg tggggtcttt cctgaagcat tggcaccgta gtaataagaa
    42601 gtgctttatt attggttaaa ggttgtgcag ctaagggata ttgattgttg aaggtatatt
    42661 ctaagcctcg tctattagta tgatctctaa tacctactgt ggcatatttt ccatgagagg
    42721 gtgaacagcc accacctcca acatctacat tattaaaggt tttatactgg aattttatca
    42781 ttccatcacc aagactggta ggatagtaat gaggatcata aaagataact tcaaaagtct
    42841 cttcagatgt acggttaaac ccatttttta gattgtggta ttctacaatg aaaatatgat
    42901 taactatatc ataatattga taaataccag cattggataa gattatcaaa tcatcccaga
    42961 agggagcaat cattggtgat ggtccttgac ctccaggcat tcttccattt ctaaattctc
    43021 cattttcagt aactccaaat gcaataaaac cattaacaca aacagtaatt tgattatatt
    43081 ctttaccgta ataaggaaaa gtgaaaggta aatttatcgt ttccagaaca tcgcttccca
    43141 cctgatctcc ctcatcatta attaaacccg aatcattcaa tccagtaata agggttccac
    43201 ttcctcctaa actgggcaca atttctatcc actcataaac tggacaatca ggataagctg
    43261 tatcagagac atcatagata aaatagccat attcatcagg acccagtggt gtattttgac
    43321 taacggttcc tataggaaga ttaaaatagg ttaattgttc aaatcctgta gaattatata
    43381 acctgagttg aaaaggaatt atcatacctg gaattaattg aggtcgtgcc ataatgctaa
    43441 aaccatcaat agaattgacc gtcataccag ccagcacatt cccgaaataa gaaatagaat
    43501 caggaacaga aactaaatca ttcattgatt gtaattcacc gtagatatca ctaatcccac
    43561 agatagaatt attggttata cttactgtta atatccctgt ttccgaagga tctaaaatag
    43621 tgtcacaacc tgcaaataca tatctgttat taacattaaa attggcattg aatgcaccca
    43681 tatggaatat tatttgatat atattttgtt gagtatcggt caattccaga ttaaaacgag
    43741 cgttgagaaa tgatgggata ttgttcgcaa agtgtaataa gaaataagta ttattcactc
    43801 ctgtatcttc tggaacaata tcaggatagg tagcttgagc ttgttgtata tttatatatg
    43861 gattagtggt gctcaatata ccttgaagtc cactgatgat atgagtagta gtatttttaa
    43921 tttcaacatt caaagctata gtctcacctg cctcaataaa actgtcccca ttacctgagg
    43981 aaccagcagt tccattatca ataatgtatt tttcaaaata aacaagggaa ccttcttgat
    44041 ttaatgacag ggtcttttgc actggtttac agttatgctg agaagcagtt atcagtaaat
    44101 tactcgctat aaaagaagga atttgtaaag taactgttcc tgtttcatca gtataacctt
    44161 tagacactac attttgatat actgaattat agagagtaac gctaacacct tccataggat
    44221 tctgaaataa atccatgata ttgatatcaa ctaatgaaca accacgagga atagaatctg
    44281 gagcattaat cagcaaaccc ttaggaattc ccacaaaagc ttcaaccgta gggtccccta
    44341 ttaaattgca ccaatgcgca aaagaattag cttgagaagt gttcgcggtg ccataaacat
    44401 tccagagata taactttcca tttaataagg cttctcctat atttcgcatt ccataaagaa
    44461 atattccatc aaaaattcca caggagagag cattattaaa cattgtgtga gttcccgtgg
    44521 ttgacatacc aatagcggtt aaagctcctt taggaacagc ttcactgcct aagcgtacaa
    44581 aagtttctgt agttgaagtc ccagcaaatc ctccagttcc acaagttaga atcaccgaat
    44641 gtggaagtct gggtccatta ttaagacttt ccgaaggact ccatccattc atattaataa
    44701 ttccacgata attaaagaaa cttactcctt gattgatgcc tgaattcatt gtggaagaac
    44761 aactactggt ataattttca atataagtat aatctggatg aattttagaa gacatttctt
    44821 taatatattt gttggaatat ataacggaga tcccagatga actgggatca ccaatcaaaa
    44881 gcatacgatt taaccaagac gctgcatcag cagatatatt aatattcttt tcataagtat
    44941 agattttatt gaagacggtt gcaagttgag atagattttc cactgagatt cttcctataa
    45001 acacatcccc aagagtatca tttcctgcta aatgcgtata aggatagtca ccttcaccat
    45061 tataattact ataattttca taccaggtag gaattatata actaccagta gtatctccaa
    45121 gcagtataat ataatcgggt ctagtctcag gattatcata ttgattttgg atataatttt
    45181 ttattgaagt attacttgtt cctccagttt gtgcagtact aactacattc acttcatacc
    45241 ctttctggcg cttccaagta acaaattcat ttaatttagt aagaaaattg gcatctgtag
    45301 tattaccata gatgagcaaa atcctgcctt gttcaggagc aataagagga ttacgatatt
    45361 catcaaaatt ggctatctgt gcctcataaa tattctgaaa gacatagcta taattatcat
    45421 aggactcaaa agggagttca ttgatacctt tatcagtagt ataattaata tgcacctgaa
    45481 tattatgata atatcgcaat tgctgtttag taaagtcata ctgacaggga tatatattga
    45541 gttgaataac ccggaaatct cttaaaatag caggttctga aaaggtaaca aaattttctg
    45601 ggaaaagatt ttgtgcttct atagttacgg gtaaagcccc ttcttcagca taatatagct
    45661 taggattgtt caaaggtatg atatctacag aatcataagt gaaagtcaca ttaaaattcc
    45721 cttgtggagg aatggctatg aaggttccca aaataggtaa ttctggcata ccattaaaaa
    45781 ctgtagaatt ttcaacccct tttaacttta tttctttaac ctcttgatta ttttgtatgc
    45841 tctccttaag agtcagctga gttgattcta actttagctt agtttggtgg ttatcagatt
    45901 taactatact aattaatttt tctgcttcca gattttccgc tattaaacaa gtctgaagta
    45961 gcaatagcga cataagaaag actaatattt ttttcatttt tatctcttta ttttatacca
    46021 aaaaacctga ttaaacctcc aggtataata tattttttag ctcttagtcc atttcataga
    46081 taagaatgcc cttatctctt gcagcaacta taagctgatt attatagaac ttagcattat
    46141 tggtatatcc agcggaagta atattttgct tcagaagagg atgagaagga ctggatacat
    46201 caaacaaata aattccaccc ccaccagaac taactactgc cttattatct tttacatcaa
    46261 ttgaggtggc atagccagta gtatcaaaat tacttactaa aagtggttca gtcctatccg
    46321 ctatatttac tatcgtaaaa ccaccttgtc tacaagcaag atacgcataa tttcctacta
    46381 ccttaacttt ttgagcttct ccgggaaccg ctatttcact tatcatttct aagctattac
    46441 gatcataaat caacaaacct ctctgttgcg ctgctatata gacatatcta tcatccaaat
    46501 caactccaga ggcagatgcc ggaggataaa tactgaaagt gctacctaac cataaggtcc
    46561 cattatattt tccataatga acattgcgtc ctgcagaata gattatctct atcacattat
    46621 catctactgg atttataatt ttctgcactt tcatatcctg aatatcctga gtggcaccag
    46681 taattgaatc tatgatgcgc aagctatcta aattgctatt atctactatc aaaatcctat
    46741 ccgtggcatc agtctcattt aaaaataaac gattatattc acccaccaca cctatcttgc
    46801 gaatcttaaa taaaggaata attgactcat ctgaactgga aagtatggta taccatttct
    46861 gactataatt tgcactgttt attattgata tcccgccttg atccaatgcc acataaatat
    46921 tctctgtatc aaatgaaata tccaagggat tacctactac ctcaatatgt ctttttaagg
    46981 ttaacatatc ttcgttctgc caaggagaat tccgtttggc acatccagct aataaaagca
    47041 caagtatggc agtaatcaat ataatatact tcttcatttt caaaagattc cttttattct
    47101 tttctctaag gagataattt aataagatgc aaaaattatt cctcatttga ataattattc
    47161 cttcattgat tttattcaac tgtctttcta aaaactaatc tcaaaaaatt ggttttcagt
    47221 aataagatta atactactcc tgaaaatata aatcagacag gataaattca aaaaccgctt
    47281 gagttcttta ggagttccaa tttaaagggt atattaactc attccttaga ctttcaggat
    47341 taaattcctt caattctcaa ttagtatcat aggcttaaac ttattcattt aatacaaatt
    47401 cccttaatca cctggattct cgctccaatc cgcaaaaact aatgaggaac ttaaacacat
    47461 aatgaccata aatgatattt ttttcatttc atatcttctt attaaactta tttactgata
    47521 gttatcataa tttttaacac cttttaccaa tagaaatttg tcaataaaaa actgtaatat
    47581 tttcacccta aataggctca ttctttcatt aatcgctatc tttgatattg aaccatctgg
    47641 gatttattct tggtgaacat tgaatccctt gtattaaaac ctaatttata gatatttcaa
    47701 taggtgttgt atctttatat tcattactat ctaaaag
//
LOCUS       DAORZF010000008        46305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_643_length_46305_cov_389.867222, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000008 DAORZF010000000
VERSION     DAORZF010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 46305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46305
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_643_length_46305_cov_389.867222"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="PK015_02005"
     CDS             complement(<1..758)
                     /locus_tag="PK015_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425332.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOL33281.1"
                     /translation="MSFNPLWLALIVVVLMIIWEIAGKHKSPQRGWIIAGVCFILGMA
                     VFGYFFQQSRQSSSLHVVGYASKTFESDMVKWTLAMQKNSDLGGLMNAYTTLNKDVNE
                     FKNLLLAKGIAEKDIDIQPPTSSPVYDNSGNITGYNVNQTLYILSKDIPTVESIALDP
                     QFFAQRSIILMQSNLDYLYSKLPDLKKEMIGAATEDAYARAKEISSASKTHLGKLIYA
                     RTGVFQITEPYSTEVSDYGFYRTNTRTKSISVTVT"
     gene            complement(779..1234)
                     /gene="tsaE"
                     /locus_tag="PK015_02010"
     CDS             complement(779..1234)
                     /gene="tsaE"
                     /locus_tag="PK015_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOL33282.1"
                     /translation="MPLPANLKLKSKQDTIKLAEFLAPILQAGDLICLYGNLGSGKTF
                     FAAALAKALGVPDFVTSPSFILFNQYQGTKYPLYHLDLFRLKNEQEFWDLGVIDLMDN
                     GITLIEWPELANKLLPESIQNLNLYFSFDGKKRTVDIDARGKFAPYFIK"
     gene            complement(1387..2961)
                     /locus_tag="PK015_02015"
     CDS             complement(1387..2961)
                     /locus_tag="PK015_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional response regulator/alkaline
                     phosphatase family protein"
                     /protein_id="HOL33283.1"
                     /translation="MKHKKQMKILWVDDEIDLLKSFVFFLEERNYDVDTCNNGNDAIE
                     KVLENKYDMVILDEMMPGLDGLTTLQEIKRINPSIPIIMVTKSEEEGLMDKAIASQIA
                     DYLIKPINPNQIIMAIKKIFQADEIRANQIGEQYTRFIAQLNQRLFNTPDWNEWANIY
                     KEMSQWEIQIDCINDDGLRQTHFLEKRNCNTEFTTYIERNYRNWLRSDDRPNMSFDVI
                     SQYIAPHFELQIPVYFIIIDCMRLDQYLAIQPYIQELFDEEMDLYYSILPTATPYSRN
                     AIFSGMLPIDIAKHFPEYWNTTEEMDNSRNRNEHQLLDEHIAELGYNLNPTSKYVKIF
                     NMEEGNFVLRKIETWNKENLIVLVYNFLDLLAHHRSRDQILQETIPNEEGLRAFTKHW
                     FLHSSLYEALKLIAKQNAICIITTDHGSIKVNRATQVIGDRDTSVTVRYKEGKNLNAN
                     ERHAIFVKKPSEFGLPSKSIVDNYIFARDDYYFVYPNAYHQYQKQYNGTFQHGGVSME
                     EMILPIATCRPKHLKI"
     gene            complement(2958..3581)
                     /locus_tag="PK015_02020"
     CDS             complement(2958..3581)
                     /locus_tag="PK015_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33284.1"
                     /translation="MSNQNKQVETMTADEKFAAIANLKNNLEDNFISLGQLLSEIKRS
                     KLFRKKGYDNFKDFVEAEYNLSGSLAGKLVKIFDLFIDEMDVDDTTVKEIGFDRLQMI
                     YPMVNKGDWEVKDEWLEIAQEKPTNELRKYIHELKLSEKEEQIDQKKIFVNQYLERMT
                     NIFNCSRNELDYKLALYFQDMDPEQIKKVVQKRERQFESELKKEESP"
     gene            complement(3584..4426)
                     /gene="panB"
                     /locus_tag="PK015_02025"
     CDS             complement(3584..4426)
                     /gene="panB"
                     /locus_tag="PK015_02025"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOL33285.1"
                     /translation="MDEIKSKNYTPSTFKKMKSEGKKICMITAYDYAMARCVQNSDID
                     LILVGDSLGMVVLGYENTLPVTMDDMVRHTAAVRRGAPNSFIIGDMPYMSYHLNLEQT
                     KINAARLMVEGGANAVKLEGGSESRLEAISAILDCQIPVCAHIGLTPQSIHIFGGYKV
                     QGKTPQAYERLIKQAVALEETGVFMLVLEGVPEALGKEVAKTLQIPVIGIGAGRYVDG
                     QVLVNNDLLGFSDLIPKYVKTYASLDKVIVDALNHYCSEVREGIFPEEDNVYFPINEE
                     KKDN"
     gene            complement(4469..4942)
                     /locus_tag="PK015_02030"
     CDS             complement(4469..4942)
                     /locus_tag="PK015_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33286.1"
                     /translation="MLLILFLCSCCCLLFSQDIREEYDLLKNYVLPDNAVPFKELTIK
                     DSLLLKDGIPFSGIAYDLYENGKLARVISFYKGIQHGPMYIWYPNGAPQLSTNYRQGK
                     LHGWFIGWYSNGSILYSMAYNQGKYVGHYQDDDDRRQTQELPEYEGDADGLNERD"
     gene            complement(5134..5730)
                     /locus_tag="PK015_02035"
     CDS             complement(5134..5730)
                     /locus_tag="PK015_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOL33287.1"
                     /translation="MKTLEELNTPIFWAEGHPGSLERDKWVISVTGKCVNPQQFTWQQ
                     LLQLPQKEVRGRLTSVTRWSVEGLWTGIALSTILDIVKMLPSCKYLRFWSIGEIYDTS
                     IPVDIALKEKSLLAHSFNKEYLSEDYGGPVRAFVPYLWGYKSAKSVVKIELMEYYIPG
                     FWEKRGYSDSGEIEPGPCRDLNADGEIKYIQGPGEVIF"
     gene            complement(5749..7272)
                     /locus_tag="PK015_02040"
     CDS             complement(5749..7272)
                     /locus_tag="PK015_02040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOL33288.1"
                     /translation="MKYLLSTEQMRELDSRTIKECNLSSRILMETAGKGCADLLVREF
                     PAQMENGILILCGNGNNGGDGAVLARWLHHYGYVVTLVNIQLGTLSSETKTNFAICQS
                     LGIKVHKVKSEKHLSWLKEMVSMHSMVVDAIFGIGFKGELEPWLKELFFYISAFAEFK
                     VALDIPSGLNSDTGNTEMAIWVDATLCIANLKLGHIIGLGREICGKLYIVDIGIPALF
                     YEDDDNYVRLFTEKDFFPPIRISTYHKNDYGKVYIFGGISGYTGASIMAARAALRAGC
                     GYAFVLYRKRLMSIYSVKLTEALNQPIPENPEDNLPDEHALQDILSNATAVLIGPGLG
                     KDEFARKLLEITIKYVDLPLVIDADGLNLLAENPDLYPYLAKPNVLITPHWGEFIRLA
                     KIEKSDLQADCLRELKAFVDKYQAQVLLKSHFSIFCNREEIWVNISGNDGLATGGSGD
                     VLSGIITSFLAQGLSISEAAINASYLLGATAEKLAKKRATASILPSDIIDNLFIKDL"
     gene            complement(7269..8828)
                     /locus_tag="PK015_02045"
     CDS             complement(7269..8828)
                     /locus_tag="PK015_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOL33289.1"
                     /translation="MKHRSKNSTLSGNSPRKRNNFQYLRLYLIFSTLVLFVVIAIYSQ
                     VLIRNAKREQEYVPRIFAKYIAYTNNYLRQSEKYALMLAEITSKTFDALNREDFQEAI
                     SNYMLFEFPQKNPIPVIITDIDSVPQLWNQVGVSPDSTYDSLSEQSRQILRDKMKSMD
                     RIEIKSGNQVVNYVYIARPLSLQEFIKNIDYSVVVTDRDKRPLYWRNVEIPEDKSWDE
                     ITPEEKSQLHKTMNDMYEVYLGDTSDQLGYIYFSTPKYLPRIRYLVFLELALAVLLVG
                     FSSYGLFLVNRTEKDTLWIGLAKETAHQFGTPITSLMGWIDLLREAPEEGISGPELSK
                     MVDYMTTDLNQLKNIASRFGKVGSQTKLVPTELHTVLQQTVSYFKERLPHLGSKMDIH
                     LISKIQGIKVMLDTELFTWAMENLIKNCIDAMSYKGGNIFITATHNNKYVYIHIRDEG
                     KGIPHSQWKKIFEPGITTKTRGWGLGLSLAKRIIEEYHQGVIKVLTSTINEGTTIEIR
                     LNIAEQEEEKK"
     gene            complement(8770..9540)
                     /gene="mazG"
                     /locus_tag="PK015_02050"
     CDS             complement(8770..9540)
                     /gene="mazG"
                     /locus_tag="PK015_02050"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOL33290.1"
                     /translation="MKEFQKLVDIIAQLRDPVSGCPWDIKQTPQSLIPNMVEELYEVV
                     EAIEDGDNVALMEELGDLLLHIVMQAQLASESGAFNIIDVLNHINDKLVRRHPHVFGE
                     LELKDADTVKKNWERLKKAEKQERKSVLEGIPRALPALIYAQRMQEKAASVGFDWDDV
                     PPILEKIDEETKELKSALETEDQTRIKEEVGDLLFTIVNLARKLHLDAESALKETARK
                     FSKRFQYIEEQYRQNGENINEASLEELNSFWELSKKEE"
     gene            complement(9537..10811)
                     /locus_tag="PK015_02055"
     CDS             complement(9537..10811)
                     /locus_tag="PK015_02055"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOL33291.1"
                     /translation="MLKLVAEKSIYLLAALAVLAVANFLQTLQIVQIGFIGVVFLAWA
                     VISLIFGISVRNSLIFCSLFFIKPIPTSFYCVLFILLITFVFEYQRSHPLVIPYPVAL
                     IVLLLTSCYGLIRAREFYYSTQYFAATAIVPLILFILLGNTKLQKEDFILWIKSIVII
                     GIFLSIIGVFMALAHPEQRLGSLWITAMTINGYYTLAFFFSIALGMEAENSKNKYFWF
                     IGAIIILLGMLYTYTRMALLAVLFGLILIIIMVKKLRYIGMLVLLLIPLIIPSSMVSR
                     IKLGLTFDYSIVIRAIAWFYAIRQIKLAPWFGMGIDVWRHWYAAIIPLDFLYAEHPHN
                     IFLKIWVELGIFGFLSYFYIIGATVRKYYLHLVKRQKDYFSTIIFIGVLALLFACLTD
                     IFIQQFSVSMLFWITLAFMYRLAKNKTQGELQ"
     gene            complement(10827..11990)
                     /locus_tag="PK015_02060"
     CDS             complement(10827..11990)
                     /locus_tag="PK015_02060"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOL33292.1"
                     /translation="MEKREFDFFELIRLIIRNRKFIIIFVAVVSVAAVIYSLVTPQIW
                     RSTATFYVIGDQTSSLPFNIEGLSGLTAGLMGTTNSQNAISAVIAMNSRQFSEKVIRH
                     FNLIDYYKITTKDSLKAMDSALKMLHTKTMKIGSDPETGLVTVSADTKDKKLSRDIVN
                     YYLQQLDIYNREEKITRGKMNREFLEARVNETRAEIDSLLLALKDFQQRHNAVDIEAQ
                     TSSLIKSYSDIIATKMSTDIELELARKNYAENSPIVLELKDRSEALAKQIKQLEAGKE
                     PLKPRYLIDIGSLPDLATQYAQLKMNLEIKTKVYEFLYPQYEAAKLEELKDLPTLDIL
                     DTPREAGLRVRPKRAVMCIIAFALAVVVSIIIVLIKNALELNKERWQEIKKEL"
     gene            complement(12045..13325)
                     /gene="serS"
                     /locus_tag="PK015_02065"
     CDS             complement(12045..13325)
                     /gene="serS"
                     /locus_tag="PK015_02065"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOL33293.1"
                     /translation="MIDLKYIRTHPQEVRNAILNKNEKANLDAILALDENRRKLQYEY
                     DQLRSKQHQVSQIIVLKKRNGEDVSELLAEMSEVAEQIRALNAQLTETNTQLENLLLT
                     IPNIPHPDVPIGKDESANRVIKLWGEKPNFTFTPLDHLELAEKNRLLDLPRGAKISGS
                     GFPVYTGIGAIWERALINFMLNFHIQKHNYTELAVPVLVTRKTMTGTGQLPKLEDDMY
                     RIEADDLFLIPTAEVPVTNFFADEILPQDRLPIKFVSYTPCFRREAGSYGKETKGLQR
                     LHQFNKVELVHFVEPSTSYQALEEIREDAEEILQALNLHYRVVELCTGDLSFASAKTY
                     DIEVWAPGLGKYLEVSSVSNFEDFQARRANIRYKDKQGKLGYLHTLNGSGVATPRLMI
                     ALWETYQTKEGTINAPEVLKEYNVPEITESSKGW"
     gene            complement(13326..14111)
                     /gene="tpiA"
                     /locus_tag="PK015_02070"
     CDS             complement(13326..14111)
                     /gene="tpiA"
                     /locus_tag="PK015_02070"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOL33294.1"
                     /translation="MRQLIIAGNWKMHKDAQSTEDFCNALAQYLVTHKQMRVLPLIAP
                     AYPFLEKALKILNGLPVEVAAQDVSSHLEGAYTGEVSANQLSSLGLHYCIVGHSERRQ
                     YHSETDAMVRDKQLRLREQSMIPIVCLGETLNQRQAGKTQEVILSQLNGCFEGIELQT
                     GKDVIIAYEPVWAIGTGLTATANQAQEAHLLIRNWLKEHYSEEIAQNVHILYGGSIKP
                     ENIYGLISEPDIDGGLIGGASLKIEDFCKMIDIAVDLINSGSK"
     gene            14484..14765
                     /locus_tag="PK015_02075"
     CDS             14484..14765
                     /locus_tag="PK015_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HOL33295.1"
                     /translation="MLKVFEYFEGKVKSIAFENSQGMATVGVMDKGEFEFVTSTVEYM
                     MVISGILTVLLPGSKDWKTYGKGESFLVPADKKYRVKVSEQTAYYCRYE"
     gene            complement(14823..15629)
                     /locus_tag="PK015_02080"
     CDS             complement(14823..15629)
                     /locus_tag="PK015_02080"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOL33296.1"
                     /translation="MDIIVNRIDAGKYWTDSGASFGVLPRAIWGKEVQTDELHRRMLS
                     LNLLLICLPERVILVDTGLGNRLSEKQISIYNPSPFMLPVSLAELGVRDIDVTDVILT
                     HLHFDHCGGVITNFGDKERLTFPRAKYWIQKSEWEMAKHPDDLNKASYDFEHQLYLLE
                     QSNQYELIEGNLDIAEGVTLIKTGGHTVGSQIVEIDTSSEFYIYPADIIPTFFHISLA
                     VTSAYDLSRTETYKAKLYIYSRLKEKKGTLLLNHDPKHWEVNFSEIKYCK"
     gene            complement(15637..16338)
                     /locus_tag="PK015_02085"
     CDS             complement(15637..16338)
                     /locus_tag="PK015_02085"
                     /inference="COORDINATES: protein motif:HMM:NF024483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOL33297.1"
                     /translation="MITIEKSGAKVEELIAEFRKEYGLKDWEFKYEIIKNPSSGLFGL
                     LPRKNAMVRFYLPDIEERVASFLKELLIKMDISYKEIKTHKEGKSIYLEIISEEESGR
                     LIGKNGKMLDAMQYLLNKVFEENSAIEHIYLDVDGYRQRQQVQFLRPFLSIFQKVRSE
                     GKPYTLEPLNASERRIIHKYIEKQKDLSTLTIGEGTKKRMVIFPAHQSASEALIHSRQ
                     QRQQGQHSPKKSNLR"
     gene            complement(16335..17978)
                     /gene="yidC"
                     /locus_tag="PK015_02090"
     CDS             complement(16335..17978)
                     /gene="yidC"
                     /locus_tag="PK015_02090"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOL33298.1"
                     /translation="MDKRTLISLLIVLILFTILQVYVFKPPTPPAESEQVQDSLAKTV
                     PDTINTPVAVPATIAKWDSLASYPDSIKTLTLENDNFTVKFSSLGASISSVSLKKYLW
                     ADKPVPVDLVPEQGRLAGISLLGLNVDQNKDLKLLNWYYTQLDSSVVFWLGDEANPLV
                     KKVYTLDDKYGILMDVAVSSSFPVYGIEYDYSDGIADTEKIKSNLKDTDYKLLLFYEN
                     ELQKYSLSKVRKKQPQGPVSAFKWAALRTKYFTIAINETGNPLTSNYFAQVSPTTGNP
                     AIVLNSFDRSPSQNWEQHFLIYAGPADYNLMKSYIDTKMELIPERGPGWLRWLSNPIA
                     WLLQFLHSFIPNYGIVIIIFSILLKIILQPLTNKSTKANLKMQSIQPQVQELQKKYKN
                     DPQRMQQELSKLYKEAGANPFSGCFPLLLQMPIFIALYNVLRYTIDMRNARFFGWLND
                     LSEPDPYLILPLIMAGFMVLQSLMSQPSKETLDKMDDQQKAMQRSTKMMTWLMPIIMF
                     FIFKSLPSGLVLYYTVFNILSVIQMYYMKKNMKKKELLK"
     gene            complement(17988..18263)
                     /gene="yidD"
                     /locus_tag="PK015_02095"
     CDS             complement(17988..18263)
                     /gene="yidD"
                     /locus_tag="PK015_02095"
                     /inference="COORDINATES: protein motif:HMM:NF013932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOL33299.1"
                     /translation="MRRTERANSTTFLEVLIPILRLPNLVIIGLIHIYQKMISPLLPK
                     VCRFEPSCSSYSLQAFHKYNFFKALGLTIWRLIRCNPFCKGGYDPLP"
     gene            complement(18224..18541)
                     /gene="rnpA"
                     /locus_tag="PK015_02100"
     CDS             complement(18224..18541)
                     /gene="rnpA"
                     /locus_tag="PK015_02100"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOL33300.1"
                     /translation="MLRLITKHKEYLEFRNPDLFLRTSHFNVPVIVSPFEFAVGITIG
                     KKIGKAHIRNKLKRRIKAWFHQHKNELPSGFKLNLIALPGAGELNWTQLCEELNELTQ
                     RLS"
     gene            complement(18545..18679)
                     /gene="rpmH"
                     /locus_tag="PK015_02105"
     CDS             complement(18545..18679)
                     /gene="rpmH"
                     /locus_tag="PK015_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOL33301.1"
                     /translation="MKRTYQPSNRSRKSTHGFRSRMATRNGRKVLARRRAKGRKTLCV
                     "
     gene            19000..20796
                     /gene="lepA"
                     /locus_tag="PK015_02110"
     CDS             19000..20796
                     /gene="lepA"
                     /locus_tag="PK015_02110"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOL33302.1"
                     /translation="MKQEFIRNFCIIAHIDHGKSTLADRFLEYTHLVNKVEVDQVLDS
                     MDLEREKGITIKSHCVRMIHSYNSQEYVLNLIDTPGHVDFSYEVSRALASCEGAILLV
                     DASQGIEAQTLSNLYLALDNDLEILPVVNKIDLPKADVEGTIQDLSDLLGCLPEEIMK
                     VSAKNGEGVKDLLDAICEKLPAPQGNIDAPPKALIFDSFFDMFRGVVVLVRLFDGRLR
                     KGERIKLFATEHEYDIEEIGYLGLKLSPQEELTAGEVGYIIANIKEVADARVGDTITL
                     AKGGCEEPLPGFKEPKPMVYSSIFPINSEDYENLVEAMAKLKLNDASLVYEKENSAAL
                     GYGFRCGFLGMLHLEIVKERLLREYNVPIIATTPSVGFLIKLKNGQEVLINNPADFPD
                     PTLIETIKEPYMETEIIVPTEYIGNVMKLAQERRGIQKDIQYLDEKRVALHYEMPLIE
                     IIFDFYDKLKTVSRGYASLDYTFLEYRPSEVVKVDILINGEKVDAMSFICHRDKAHSW
                     GKSVTDTLAEVIPRHLFKIALQAAIGGTIIARSTINPLRKDVLAKCYGGDVTRKRKLL
                     EKQKEGKKKMKEIGSVSVPQEAFLAVLKADRD"
     gene            20807..22465
                     /locus_tag="PK015_02115"
     CDS             20807..22465
                     /locus_tag="PK015_02115"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HOL33303.1"
                     /translation="MKKFWLIFLALGVLSFNWAIIVEKISFHTNFSIDEDKLLQASGL
                     EIGSEFVPEEINRATISLQTWLQNQGHPFVQVSYPDLIPLSETGMELSFHIEEVIPAE
                     HCFLHFSGLRYFSEAKLRDLLLISDKKEVSVNELTRLMENILREYHQRGFLFAAVQLD
                     SLVLNNHLEAYISIQEGKPFKPEIYFIQGNKYTKDKTLIKLSGLNELKVITPEAIETA
                     QKNILSKSYIQSCHIEPLDPGSILIKVEEGKMTYLEGIMGLNNRNEKTELTGYLNLKF
                     LNLWGSDRSIALNWRQNPSSSNLEFAYHESGPINIPLSGDLALSRMVQDSTWIKSSVQ
                     TDIYSYHAKQKYGIELASEEISPGSRRPILVEKSSANSIGAFWQLDTRNQIFNPTRGI
                     EIGINYTLKYLTSERQWINSLETYYTQYLNISRRFNASMGFHLCSLDDSTSAEYLWYP
                     MGGFNSLRGYQEDEFISWRLAWSNLEIHYLINPDAMLYCFFDYGIYARTQTSINAKLF
                     APGLGLRVRTRLGILRMEYGLGYREDGFPSLASGMVHAGIETSF"
     gene            22672..22842
                     /locus_tag="PK015_02120"
     CDS             22672..22842
                     /locus_tag="PK015_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33304.1"
                     /translation="MKKIVVIALIATMLIGMLVFSACKSKETQEQGIDTTAVIDTTQE
                     VSPDTTVVPMPE"
     gene            complement(23014..24549)
                     /locus_tag="PK015_02125"
     CDS             complement(23014..24549)
                     /locus_tag="PK015_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOL33305.1"
                     /translation="MIEVSLAPDGTGYPLPTELDNAKERETLLKITEEQRKLGRKIVV
                     VQGLGFVGCVMAAVVADATDEDGNPYYYVHGHQRPSKRSYWKVPMINSGVPPVNSSDP
                     EIPQIFKRTVIEKKNFRATSEEFVYSLADIVVVDIQLDATKPSFGEAEKGYCDLLAFR
                     EGIRTLGERIRPNCLVMVETTVPPGTCEKVVKPILEEEFTKRGIDIALHPPLIAHSYE
                     RVMPGANYVASIRDFWRVYSGVNPESAELCREFLSNVLNVKEYPLTQLDNTNASELAK
                     TMENTYRAVNIALTLEWARYAEQIGVDLFKVREAIRKRKGTHDNMCRPSLGVGGYCLT
                     KDPVLANWAMQTLFEINDFLEMAIRSVNINDTMPLHTIDIIKKEIPALRDVKVAVLGV
                     SYLENVADTRHSPSKTLVEFLRKEFALVYTHDPYVEVWPELEDAKVEKDLNSVLPQAD
                     VVIFAVGHNQYKHLDPAEVVAMCQGTKPLIVDCSNFLNDEVINKYKQLGCKVRGVGKG
                     HII"
     gene            complement(24744..26147)
                     /locus_tag="PK015_02130"
     CDS             complement(24744..26147)
                     /locus_tag="PK015_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOL33306.1"
                     /translation="MEKAKKHNNIFILEEMPIFQAILSLALPSMLSMLINILYNLTDT
                     FFIGQLKDPNAMAGVSIALPLFTMQAAIAGIFGVGGASYLSRLLGKKDYYQAKETATI
                     SIFSTFIISIIVSIVGILTMPYTLKAVGASAATIVPASKYMFWMFLGSPIVFVKFTMV
                     QMIRGEGGAKYSMYGLFIGTGANIILDPIFIFALNMGVSGAAIATVIGQGLAMLYYIW
                     YYHSTHAVAIPGKKYLHFKWNIFKEILYIGIPASLSQIMMSVGNAITYRLASSYGDAS
                     VASLGVAFRVFSIPLFIFIGMAMGVQSLIGFNYGAGNYSRMKKALRSAVIINLGLSAI
                     FTLIFILFPRGLIGIFIHNEEIMDIGKKVLEAYVWAIPFASVGMIFMNSLQAMGKALP
                     ALIVSLSRQGLIYIPAVLIMNKFFGFNGLVYAMPMGDAFTTILSFIFVYHIIQKLKTA
                     PAAPISQEYIPFPADEA"
     gene            complement(26827..30897)
                     /locus_tag="PK015_02135"
     CDS             complement(26827..30897)
                     /locus_tag="PK015_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33307.1"
                     /translation="MKHSRVFLTLILILLLINVLFFVSWYLLDVQGKVKNLIEHEVGK
                     ALKGQMQIGNLSIGERQIFAQNIQFASTDGTFKFTVDNAHCRYNLLKFIFSGFKIRNI
                     LDKVEVDGAEVSYSYPPLVKKPKKKLVIPDLCLYFNQATVNNARFLLALDYPLKIGTD
                     STLSIREQFDHISLNMVNTDVSNFKLSGQSAKDGTIRANGVLDKGSLSSLQTELRNYH
                     PQYINHPQFTNLDTEIDLTLKASQKNKDAKLEFETKTFLHQTEVLLASQFPLYIPSLH
                     ILGNQDRLVVELSPSTVGTSAIGGRVVVTGITDKIGLEPSELKLKLDLAMLTPELQGI
                     VDASLSADGYFSEPQLTLNAASEKVSYANQDFQNLHLQAFYLDQIASFTLNNLIWQNQ
                     NINLYGDYSLENRQLTGFLNTTPINHHPQEMKITADADFELNFATSVPEVKAQINKLS
                     YAQNEIKLDAINGYANFLPLISESQKNYYIDVALISSQGFNLSLIGDIQDKNFLLDAK
                     CKDLDISRLYANKAITDIAPEITGDIQAFLTGNKLVLASKLELELKSNVDLQSNIDLI
                     ASYDLKNNEGTIILDAPSELDNNTVNLELNANLKNTVIDIQRLKINDQIYARGQVDVK
                     NVKNPYLQLEVQNVDYQDIKNYFPALNLPEFQNLNLSLEYDSDKEKPLNANLKFNDLI
                     IPGLQPVSSNLNFQGPLQQVQLNGTINNPSKALASLSGSLNIVDNLDIRLQAIIPDLN
                     MRDLMYSPPVEGNVYGNVGLFIIDTLSPDREIILDVNVKSNQLAVPDIAQLDDIQIML
                     SQTKNVLIVDTLYVQCNELGTVSGSGAIDYNLLTNTYFEGNHTLQLKVDGLLFEWLDK
                     NVPLVKNARGKAHLDCSLQTFEDQLMVKNGNFSINDGSILLQDQAEPIRNINISAKIQ
                     DNLIFLENFTCYMGEGKLTVKNSFDYDESIHLNVGFLDLGTLALKIDEPGALIYIPEI
                     TTPLSLSKVIIRGQNTEYATIYGPFEDMTISGEILVYNANIVFPPNTNNLLNLIYNIR
                     SSIIKDEEKTKEPVPLPFTLDLMIRLGENNKYATYPTNFYLQPGSFLHLIYDGHKWKV
                     AEASISSEQGTLDFFGTIFQTETIALEILESQNKIDIKGRFNYRSPDGTIVTLDVGMD
                     PDVSKPLLQRLIFALSSDNENDITIASIIQRETMLRGKTSQNTPEEQNILRQEAVSMI
                     SENLYYSFISPVIYPLENNLRRWLKLDNFSIKIGFIQNLFNEYTTSPYPLAEYTDMNQ
                     FMNDIVQFSSSILLNNLSISMSKYLGLRMFLDYQFTLQEATDLQSTKFLIYHDTSLRW
                     ILPRQYRITYTFKYEPITPKMTHEIMAHKSLRFWSI"
     gene            complement(30902..31567)
                     /locus_tag="PK015_02140"
     CDS             complement(30902..31567)
                     /locus_tag="PK015_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOL33308.1"
                     /translation="MLCLAGYSLYKTYADIGNDIRVLRGTNLEVECGDFVCITGKSGC
                     GKSTLLHILGLLDEPDSGKVFIKGVEVSTHHPQANQIRNQQIGFVFQFHYLIDDLNAR
                     ENVVLPMLIAGANKSVAFQKADELLRLLELSDRAWHYPNQLSGGEQQRLALARALINQ
                     PHIVLADEPTGNLDPQHSTEIWNLFQRLNRELGQTFVIVTHDPELAKLAHKSYELRDG
                     QLF"
     gene            complement(31580..32797)
                     /locus_tag="PK015_02145"
     CDS             complement(31580..32797)
                     /locus_tag="PK015_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOL33309.1"
                     /translation="MNPAEKLFLERYIQAPKKHLWRFSFLFMVLGIIISVGILSTALN
                     LFEGYEHALKSVLLDSFAHIRIEHINGELLSDTLISSTLQELNSRPEIDSVTPLLTSN
                     LMASNGTKARGTIFNAYDVNYASKVNYAKYVKEGTAQLAKGNIIVGTYLAKELGLSLG
                     DTLKVFYPRLDKITPLGMYPVETPLIITGIYHSGFYEYDRTLIIGTLEDARILSGAKD
                     GFSCLEIRLKNNYADDINLYAEEYDKIMDSNLVALPTTNTTLLRLVKMQKWLIFIVFF
                     FLVIIAGINIISGISTQIYDKKNEIAVLKALGAPKSTIRHILGYQVTLLCLISIILGL
                     ILGFIASWLIVHQNIYQLKGEVYFIDKLALYIAPLNQILIFILASILVFLCVQIPLRK
                     IDKMQVIDLLRNP"
     gene            complement(32784..33746)
                     /locus_tag="PK015_02150"
     CDS             complement(32784..33746)
                     /locus_tag="PK015_02150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOL33310.1"
                     /translation="MLLSFVIPVYNEQDSLKQLTDEIIANCAPHNYEIIFIDDGSQDH
                     SFAIMEELACQDPNIKVVKFRRNFGKAAALQYGFNLAQGEIVFTLDADLQDNPKEIPK
                     FLDKIAEGWDLVSGWKQKRNDPWHKVLPSRFFNFVTARAFHLKLKDFNCGFKAYRREV
                     VKELSLYGEMHRYIPALAHSLGYKITEIPVEHRKRIYGHSKYGMERYLRGFFDLLTVK
                     MVTQYAKSPLYLFGRVGLIATLIGTIIAIYLTVLKLFYNQPLSNRPLLFLAITLILGG
                     LQFVSLGLISELIVNRSVPLRPLPVSVEKTVNIQNKEGLDLESS"
     gene            complement(33758..34342)
                     /locus_tag="PK015_02155"
     CDS             complement(33758..34342)
                     /locus_tag="PK015_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOL33311.1"
                     /translation="MKFKESLRKTIHLSSLIIPLGYRYVLNYNRNLMFSIMLVVFILM
                     LVVEFYRLWQKSFRKTFWLIFGMVLRHKELYDFTGATYLFFSSVICIAFFPPEVTFFA
                     LCFLSLGDTSAALVGLNLGKRKYKNSNKTLEGSLACFIVCFVFALCFKADPVIALIGA
                     LGATLAEASRIRIDDNIKIPFLSAILMTLAGIFI"
     gene            complement(34366..35925)
                     /locus_tag="PK015_02160"
     CDS             complement(34366..35925)
                     /locus_tag="PK015_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOL33312.1"
                     /translation="MVENLSEGKPPSLLEKIIQKRGLSIESLEAGLEALPDEALLANI
                     EKCAARIKKAMYENEPLVIFGHDDPDGITSAYILYQFFNTCGFQNHRYFIPNRNVEPY
                     GIQDSLVNFVKEGNYHLVITVDNGIASFEGVEKLNQIGCETLITDHHLIQPDKIPNAY
                     TIVNPHLPSSTYPYKMLAGVGVVLMLIRYLGRELEYDITPDYYFWTAVGSIADRVPMT
                     DVNRIIVRYAFQHWQELKDSTFEFLVPYYGPIETYSDIYELIQYLVRLLSNGREENGE
                     NAALRFLLETNGEKEKLFYHLDSLKNQSETELAKVYNFLDTLTTGFEGSAFIYFDDEG
                     IIPYHLLGAAASYIAGKFGVPTLIMKLHNSEIVCEGRCNEGFNLVEAFTFCKEHLKQY
                     GGHAKAAGFSLEPEKYDSFLECFHQYLDENCPEPVTNLKIEPDAVVSLEELNEQNWLS
                     LELLLPYGQDHPEPCFLIKDTTLMELQSKFQVDINGFNFPDNIKGDVLVSWISPRVLK
                     VLSFTETSSIA"
     gene            complement(35919..37148)
                     /locus_tag="PK015_02165"
     CDS             complement(35919..37148)
                     /locus_tag="PK015_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33313.1"
                     /translation="MKKIIFLISALGILTFTLSAGNSIFSYDGYPVQYYGTDIYSLGM
                     GDTGASDIFRVNNGFANPAMNNPDNRCFFSTGIIPGYNSYRSKDEQGNTKSFLDNSLD
                     FPLFSLSFPLKLHRFGFQFNPYVSGLVSNEHTFLLEDGTEVVEKQSIDRYIYRADLIY
                     SYRWKNNSFGVSGNYFFGHDIRDFKQEGNFGLFNTREKLSRDFKNPTFTLGYLWHNDK
                     LALGAHYTMTTKLKGEEVRSSIHQTEEPISYTLQIPSQYSLSVTALPLSQFKVACDLH
                     YETWEAIDENKYTDSWKAGLGFAYEPSASDEHKGMLKFPMRTGVSYRHLPFQVNEADV
                     NELALSLGISLPLPVSVNRIDLGFQYSQRGNLEKNRLLDNSFLFMIGFTGFDIFSKPI
                     DRTVPRDIPEKEETTLW"
     gene            complement(37145..38038)
                     /locus_tag="PK015_02170"
     CDS             complement(37145..38038)
                     /locus_tag="PK015_02170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOL33314.1"
                     /translation="MRLFETHAHLDLPDFDSDRESLIQECFNSGIEYIINVGFNNETS
                     LNALELAKKHLHIFSTVGFHPHEATDFDAEVVKYLAREKKVLAVGEIGLDFFRNLAPY
                     SVQREVFSNQVLLAVDYDLPIIVHDRDAHQECFKILKQANAKDVVFHCFSGDLIFAQQ
                     VLDEGWMISFTGSITYPNSRLDDVIRMMPLDQFMIETDCPYLTPHPHRGQRNSPLYLH
                     LVAEKIADIRGITPNEVAEHSFNNAFRFFRIPPDPIKPPRGKSSHKTSVTAKIAKSRK
                     NFMAPSTGKTLTKIKKGQNKK"
     gene            complement(38277..41798)
                     /gene="nifJ"
                     /locus_tag="PK015_02175"
     CDS             complement(38277..41798)
                     /gene="nifJ"
                     /locus_tag="PK015_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOL33315.1"
                     /translation="MADVKKATMDGNTAAAHIAYAFAEVAAIYPITPSSPIGELVDAW
                     AASGKKNLNGTTVDVIEMQSEAGAAGAVHGALSAGAVTTTFTASQGLMLMIPNMHKIA
                     GEMLPTVFYVTARSLAAQSLSIFGDHSDVMSARNTGFAFLACNSVQEVMDLGLVAQLA
                     TMKTSIPFVVFFDGFRTSHELQKIEVIDYDTIAELNDPKLVEAFRQRALNPDHPRIKV
                     GQENPDVYFQGRETVNGHYLAAPDIVAETMKQVGDKIGRYYQPFEYVGHPEAEYITIA
                     MGSGCETIEETITFLNKERNMKLGLIKVHLYRPFSIDYFLSVLPSTVKGIAVLDRTKE
                     PGSIGEPLYLDVVSALKNRPDLYIIGGRYGLSSKEFTPPMVLAVYKHLMEKGFHSFTV
                     GIEDDLTHKSLPFDEPLDPSPKDTINCKFWGLGSDGTVGANKNSIKIIGDHTDLYVQG
                     YFQYDSKKSGGITRSHLRFGKSPIKSQYLVYQEDFVACHNPAFIGRFDLLEGIKENGV
                     FLLNSHWNREEVFENLTRKMQEVIINRKIQFYNINALQIAEDVGLGGRINTVMQTAFF
                     LISGILERNEAIALIKDSIRKTYSRKGEDVVNMNLLAVDRVNEALVKIDIPESLPEKF
                     APDKKLVPDDASDFVKRVIEPVMREKGDQIKVSDMPLDGVIETGTAKLEKRRVAPLVP
                     HWIAEKCIQCNQCSFVCPHAAIRSKLIKEDDLKDAPASFNTLKAIGAEGYQYKIQVYI
                     DDCQSCKVCVNECPKAALEMSPIEDERAAGEQENYEFFEKLPYDYTGSFKETTVKGSQ
                     FKQPLLEFSGACAGCGETPYVKLLTQLFGDRMIIANATGCSSIWGGTFPTIPYTKNQS
                     GKGPAWANSLFEDNAEYGFGMRLAVNSNRKLLQIYLEKLLEKELKPELKEAINYALEH
                     WDAVDKQAKENADLIKKLLPEALNSAEGDSKALLQKVIELQDNLIDRSIWAIGGDGWA
                     YDIGYGGLDHVMASNRNVNILVLDTEVYSNTGGQASKSTPLGAIARFAEAGKNTNKKD
                     LGMMMMTYGYVYVASIAMGANKVQAINAILEAEAYPGPSIIIAYAPCINHGIDMSYSQ
                     KRQKTAVDAGYWLLYRYNPLNALQGKNPLTLDSREPTLSVKEFLKSERRYAALKDIAP
                     ERSEMLQNEAEEFFKERYLQYKKLAQS"
     gene            complement(41919..44750)
                     /locus_tag="PK015_02180"
     CDS             complement(41919..44750)
                     /locus_tag="PK015_02180"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOL33316.1"
                     /translation="MNLKKLIFISVLFCALFTILPALPNELLEQPLQQDPAILSGQLP
                     NGFTYYIQHNPNPSHLAELRLYVDVGSVDEDDDQRGLAHFTEHMVFNGTKNFAKSELV
                     NYLSSIGMGYANGLNAMTSYDYTIYTFKIPTDDKEKLNKGLMILSDMAYQATFDPAEI
                     EKERGVIIEEWRMGQDAESRIRDKQNSVILAGSRYAERSPIGTFEVISSFTRDTLLRF
                     YKDWYRPDLQKLVIVGDFDPQEILALVTQYFGVIPKPVNPRPKQEFKVPENLTPVAVV
                     ATDPEYPYNILNVMWKKEPSPVQTYGSYLNYLKNSLFYTMLNSRLDEFSKSPNPPYSF
                     AAAYEYPMLRTMSTAAIMSMFLPNKAETALTTMLTEAERVQRYGFLDSEFERAKSQIL
                     RELQQAVDQKETRESSQIAWELVFDLSYNDVTLSAEQDLELTKSFLTELTLPEINAIV
                     TQLIQEKNMCISIGAPEKEGLTYPSEERLLEIAQHISTQELEAYEDKALSAPLMSHLP
                     KPVKIKKESYDPETGIYYWQLKNGIKIYAKKTDFKNDEVLLSAISPGGYTRYNKEDIP
                     AAELVSSYLKESGFGSFDAVSLSKATADKVVRVSLDVGPYTETLDASCSPKDMELMFQ
                     MIYQYTTNPRFNAQDFQSFIQRTKTYYENRNLDPLNVFMDKIDKELYNNHPYRVNLAE
                     VDLDKIEMSTVERIFRDRFADLSDFSFIIVGNFDEKLLKQYCQIYLGNLPTKKRKEQI
                     KDVNIYPISGQKEVRFSKGATDRAFVSHITNGSYKPNIENDVLMDGLLYVMNEKLREN
                     IREERSGVYLIQAWSDMTPYPKPYYLLNIVMACAPVRVDELNNAIFATLDSLQSNFID
                     ERYIISARETLKQQFKQNIRSNRYWINQLRTNIWLGKPLTEFTNRPQYIDRLTPESIV
                     SAANSYLRFNDNKLSVIMLPENGKKDSKN"
     gene            complement(44774..45394)
                     /locus_tag="PK015_02185"
     CDS             complement(44774..45394)
                     /locus_tag="PK015_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33317.1"
                     /translation="MKKLTLWILPLMALWLITGCDSGGAFRVINQTSYPLYVKVDEQD
                     EVTIPGNSNYTFEIETKKESIFNPDVEKKVPVWMIGETYQIYDDYTQSYTDSTTVTIK
                     VNKTINAYIQPNRASIKVVNNSDLWIAKIDIYKHNFIGVVNHYTIDPIAPHSFNFKNV
                     DYVTPNNNFYYFVLIELEDGTRYQYGGENAILQKDQQFLVTVENPQ"
BASE COUNT        14470 a         8527 c         8432 g        14876 t
ORIGIN      
        1 gtgaccgtta cagatatact tttagtacga gtattagtcc tgtaaaaacc gtagtcagaa
       61 acttctgtgg aataaggttc cgtaatctgg aaaactcctg ttcgtgcata aataagtttt
      121 cccagatgag ttttagaggc ggagctaatt tctttagcac gggcataagc atcttcagta
      181 gcggcaccaa tcatttcttt tttcaaatca gggagttttg aatagaggta atccagatta
      241 gattgcatta atatgataga acgctgagca aagaattgag ggtccagagc gatggattct
      301 accgtaggaa tatctttact aaggatatag agagtttgat taacattata tccagtaata
      361 tttccgctgt tatcatatac gggagaactt gtaggaggtt ggatatcaat atctttttca
      421 gcgatacctt ttgcaagcag taaatttttg aattcgttca catccttatt gagtgtggta
      481 tatgcattca tcaatccccc taaatcagag ttcttttgca tagccagagt ccatttaacc
      541 atatcacttt caaaggtctt gctggcgtaa cccacaacat gtagagaaga cgattgtctg
      601 ctttgctgga agaaataacc gaaaactgcc atacctaaga taaaacagac accagcaata
      661 atccatcctc tttgagggga tttatgcttt cctgcgattt cccatataat cattaaaacg
      721 actactatca gtgctaacca taaggggtta aagctcattt tatcctcctg agcataattt
      781 atttaataaa atagggagca aatttacctc tcgcatctat atccacagtc cgtttcttcc
      841 catcaaaaga gaagtataag ttcagatttt gaatgctttc aggcagaagt ttatttgcca
      901 gttcgggcca ttctatgaga gtgataccat tatccattaa atcaataact cctaaatccc
      961 agaattcctg ttcatttttc aggcgaaaga ggtccagatg atataatgga tattttgtac
     1021 cttgatattg attaaataaa ataaaagaag ggctggtaac gaaatcagga actcctaaag
     1081 cttttgccag tgcggcagca aagaaagttt tacctgagcc cagattacca taaaggcaga
     1141 ttaaatctcc cgcttgtagg ataggtgcaa gaaattctgc taatttaata gtatcttgct
     1201 tgctttttag tttcagatta gcgggtagtg gcatagattc ataaccttat ttctgacaga
     1261 acatttataa aagaaaaagt cccaaaagct gcaagcacaa ttatcgttgc ttcttacatc
     1321 ttttgggacg atatgcacat tgcacaatcc taaaatccat taatagaatt ttactgctta
     1381 tgtcaattaa attttaagat gctttggacg gcaggtagct atgggtaata tcatttcttc
     1441 catagagact ccaccgtgct ggaaagtgcc attatactgt ttttgatatt gatgataggc
     1501 attaggatag acgaaatagt aatcatcccg tgcaaagata tagttatcca caatgctttt
     1561 acttggaagt ccaaattcgg aaggtttttt cacaaagata gcatgtcgtt cattggcatt
     1621 aagatttttt ccttctttat aacgcactgt gaccgaggtg tctctatcac caataacctg
     1681 agtagcgcgg ttcactttta ttgagccatg atcagtggta ataatacaga tagcattctg
     1741 tttggcaata agtttaagag cttcataaag ggaagaatgc aagaaccaat gtttagtaaa
     1801 ggcacggaga ccttcttcat tgggaatagt ttcttgtaat atctggtcac gagaacgatg
     1861 atgtgctaaa aggtccagaa agttatatac cagcacaatc aggttttctt tattccaggt
     1921 ttctattttg cgtaggacga aattaccttc ttccatattg aagattttca catacttaga
     1981 agttgggttc agattgtaac ccagttctgc aatatgttca tccaaaagct gatgttcgtt
     2041 tctattacga gaattatcca tttcttcagt agtattccaa tattcaggga aatgtttggc
     2101 tatatcaata ggtaacattc cactgaagat agcattgcgg ctgtagggag ttgcagtagg
     2161 cagtatagag taatagaggt ccatttcttc atcaaagagt tcctgaatat atggttggat
     2221 agccagatac tggtctaaac gcatacaatc tataataatg aaatatacgg gaatctggag
     2281 ctcaaaatga ggtgcaatgt attgagaaat aacatcaaaa gacatatttg gtctgtcatc
     2341 agagcggagc cagttacgat agtttcgttc tatataggtg gtaaattcgg tattgcaatt
     2401 gcgtttttcc aggaaatggg tttgacgcaa gccatcatca ttgatacaat ctatctggat
     2461 ttcccattgg gacatttctt tatagatatt agcccattca ttccaatcag gagtgttaaa
     2521 aagacgctga ttgagttgtg caataaaacg tgtgtattgt tctccgattt gattagcgcg
     2581 tatttcatca gcctgaaata tctttttaat tgccataata atttgattag gattaatagg
     2641 tttgataagg taatcagcga tttgagaggc aattgcctta tccatcagcc cttcttcttc
     2701 actttttgtg accataataa taggaataga aggattgata cgtttgattt cctgtagggt
     2761 ggtcaaacca tctaagcctg gcatcatttc atccagaatc accatatcat atttattttc
     2821 cagcaccttt tcaatagcat cattgccatt gttacaggta tcaacatcgt agtttcgttc
     2881 ttctaaaaag aagacgaaag attttagaag gtctatttca tcatcaaccc agaggatttt
     2941 catttgtttt ttgtgtttca aggggattcc tcctttttta attcagattc aaactggcgt
     3001 tctcttttct ggaccacttt ttttatttgt tctgggtcca tatcttgaaa gtaaagtgcc
     3061 agtttatagt ccagctcatt acgagagcag ttaaaaatat tggtcattct ttccagatac
     3121 tggttaacaa agatcttttt ttggtcaatt tgctcctctt tttcacttaa tttaagttcg
     3181 tggatatatt tacggagttc gttggtaggt ttttcttgtg ctatttccag ccattcatct
     3241 ttcacctccc aatctccctt attaaccata ggataaatca tttgtaagcg gtcaaaacct
     3301 atttctttga ctgtggtgtc atccacatcc atttcatcaa taaagaggtc aaagattttg
     3361 actaattttc ctgccaagga accgctaaga ttatactctg cttcaacaaa atctttaaaa
     3421 ttatcataac cttttttacg gaacaatttg ctacgtttaa tctctgaaag cagctgtcct
     3481 aagctaataa aattatcttc caaattattt ttgagattag ctatagccgc aaacttctca
     3541 tcagcggtca tagtctcaac ttgtttattt tggttactca ttgttagtta tccttttttt
     3601 cttcatttat cgggaaataa acattatctt cctctgggaa aattccttca cgcacctcgg
     3661 aacaataatg gtttaaagca tcaactataa ctttatccag cgaagcatag gttttcacat
     3721 atttgggtat taaatctgaa aatccgagta aatcattgtt aaccagcact tggccatcta
     3781 catatctgcc tgctcctata ccaataacag ggatttgtaa ggtttttgct acttcttttc
     3841 ctaaggcttc tggaactcct tctaaaacaa gcataaaaac acccgtttct tctaaagcca
     3901 ccgcctgttt tatcagccgt tcataagctt gcggagtttt accttgcact ttataaccac
     3961 caaatatatg aatactctgt ggtgtcaagc caatatgtgc acaaacggga atttggcaat
     4021 ccaggatagc agagatagct tcaagtcttg attcggagcc accttccagt ttaactgcat
     4081 tagcaccacc ttctaccatc aatctggcgg catttatttt agtctgttcc agattaaggt
     4141 gataactcat ataaggcata tcacctatga tgaaagaatt tggagctcct ctacgaactg
     4201 cagcagtgtg acgaaccata tcatccatag ttacaggtag cgtgttttca tagcctaaaa
     4261 ctaccattcc cagactatca ccaaccagga ttaggtctat atcactgttt tgcacgcatc
     4321 ttgccattgc gtaatcatag gcagtaatca tacagatttt cttaccttca gatttcatct
     4381 ttttgaaggt ggaaggagta tagtttttac tttttatttc atccatggta tataaatcct
     4441 ttctatttaa acctgaagat ttggagcctt aatctctttc attaaggcca tctgcatctc
     4501 cttcatattc tggtaattct tgcgtctgcc ttctgtcatc atcgtcctgg taatggccaa
     4561 catattttcc ctgattataa gccatactat ataggataga tccattgcta taccagccta
     4621 taaaccatcc atgaagttta ccctgacgat aattagtgct caattgagga gcaccattag
     4681 gataccatat atacatagga ccatgctgaa ttcctttata gaaacttatt actcgggcaa
     4741 gtttcccatt ctcatatagg tcataagcta ttccactgaa aggtatccca tcttttagta
     4801 acagactgtc ttttatggtc aattctttga aaggcacggc attatcgggt agcacataat
     4861 tttttagtaa gtcatattct tcacgaatat cttgagaaaa gagtaaacag cagcaactac
     4921 agaggaataa tataaggagc atcctgataa aagctccttt caattttgtg gaaagataat
     4981 attttctttt aattttcata tccttattga taccatagca attttaactt cttagaagtc
     5041 aagaatgatt atctatatat aagttaaagc aatttcaggg tatggcaata gatgtgaata
     5101 atcttcttga tttcctggta aaccgttctg tagctaaaat atcacttctc caggtccctg
     5161 aatatattta atttcaccat ctgcattcaa atccctacag ggaccaggtt caatttcacc
     5221 actatcagaa taaccccgct tttcccagaa accaggaata taatattcca ttagctctat
     5281 tttaactact gatttggctg atttataacc ccaaagataa gggacaaatg ctcttacagg
     5341 tccaccataa tcctcactca ggtattcctt attaaaacta tgagctaata aggacttctc
     5401 ttttagcgct atatctactg gtatggaagt atcatatatt tctcctatag accagaaacg
     5461 taggtatttg caggagggta acatttttac tatatctaag atggtagaga gtgcaattcc
     5521 agtccagagt ccttctactg accagcgagt tacactggtt aatcttcccc ttacttcttt
     5581 ttgaggtagc tggagaagtt gttgccaggt aaattgttgg ggattaacac attttccagt
     5641 aacactgatg acccatttat ctcgttctaa cgaaccggga tgcccttctg cccaaaatat
     5701 gggagtgttc aattcctcaa gagttttcaa gataacatcc ttttaaattt ataaatcctt
     5761 aataaacaga ttatcaataa tatcagaagg gaggatggag gcagtagcac gtttttttgc
     5821 cagcttttct gcagttgcac ccaataaata ggaagcattg atagctgctt cagaaattga
     5881 taaaccttga gccagaaaag aggtaataat tccagataag acatccccgc ttccacctgt
     5941 tgctaaaccg tcattaccgc tgatattaac ccagatttct tctcggttac aaaagatgct
     6001 gaaatgactt ttgagcagaa cttgcgcttg atatttatcc acgaaagctt ttaattctcg
     6061 tagacaatct gcttgtaagt cgcttttttc tattttcgcc agacgaataa attctcccca
     6121 gtgtggtgtg attaaaacat tgggcttggc taaataaggg tataaatccg gattttctgc
     6181 cagaagattc aaaccatcag catcaattac cagaggtaag tcaacatact taatagttat
     6241 ttccagcagt tttcgggcaa attcatcttt acccaatcca ggaccaatta atacagcagt
     6301 agcattggaa aggatatcct gtaaagcatg ttcatcgggc aaattatctt caggattctc
     6361 cggtattggt tgatttagag cttcagttag tttaactgag tagatagaca tcaatctctt
     6421 gcgataaaga acaaaagcat agccgcatcc agctcttaag gcagctcttg cagccataat
     6481 agaagctcca gtatagcctg aaattccgcc aaaaatatat actttaccat agtcattttt
     6541 gtgataagtg gatatacgga taggagggaa gaaatccttt tcagtaaaga gtcgcacata
     6601 attatcatca tcttcataga ataaggctgg gataccgata tcaactatat acagttttcc
     6661 acagatttct ctgcctaaac caatgatatg tcctaacttt agattggcaa tacataaagt
     6721 ggcatcaacc cagattgcca tttcagtgtt gcccgtatct gaattcaaac cggaaggtat
     6781 atctaaggca accttgaatt cggcaaaagc actgatataa aagaataatt ccttaagcca
     6841 gggttccaat tcacctttaa atccgattcc gaagatggca tctaccacca tactatgcat
     6901 agagaccatt tccttcagcc aactaagatg tttttcggat ttaactttgt gcacttttat
     6961 tcccagtgat tggcatatag caaaatttgt ctttgtttca gagctaagag tgcccagctg
     7021 gatattcacc agggttacta cataaccata atgatgcaac cagcgagcaa gtactgctcc
     7081 atcacctcca ttatttccat ttccgcaaag aataaggatt ccattttcca tttgagcagg
     7141 aaattcccgg actaataaat ctgcacaacc cttacctgca gtttccataa gtattcttga
     7201 agatagattg cactctttaa tggttctgct atccaattct ctcatttgtt cggtagagag
     7261 taaatatttc attttttctc ctcctcctgt tcggcgatat ttaatctgat ttctatagtt
     7321 gttccttcat tgatagtgct ggtaagcact ttaatcactc cttgatgata ttcttcaatg
     7381 atacgttttg ccaaacttaa tcccagaccc cagccgcgag ttttagtagt tatccctggt
     7441 tcaaagattt ttttccattg agagtgtggg attccctttc cttcatcccg gatatgaata
     7501 tatacatatt tattattatg tgttgcggtg ataaagatat ttccaccttt atatgacatt
     7561 gcgtcaatac aattttttat caggttttcc atagcccagg taaaaagttc ggtatccagc
     7621 atcaccttta ttccctggat tttggatatc agatgaatat ccatttttga acccagatgt
     7681 ggaagacgtt ccttaaaata agaaacggtt tgttgcaaga cggtatgtaa ttctgtcggg
     7741 accagtttgg tttgagaacc taccttcccg aaacgagagg cgatattttt taactgattt
     7801 aaatctgtag tcatataatc caccatctta ctcaattctg gaccggaaat tccttcttct
     7861 ggagcttctc ttaacaagtc tatccatccc ataagagaag ttatcggagt tccaaattga
     7921 tgagctgttt ctttagctaa gccaatccac agagtatctt tttcggttct atttacgagg
     7981 aataatccat agctactaaa acctaccaat aatacagcca gagccagttc cagaaagaca
     8041 agatatctaa ttctgggtag atatttagga gtggagaaat agatataacc taattggtcg
     8101 gaagtatcac ccaaataaac ctcatacata tcattcatag ttttatgcag ttgtgatttt
     8161 tcctccggtg ttatctcatc ccagctttta tcctcgggaa tttcaacatt tcgccagtag
     8221 agtggtcttt tatccctgtc ggtcacaacc acagaataat caatgttctt aataaattct
     8281 tgtaaagata gaggacgagc gatatagaca taattaacca cttggttgcc tgactttatt
     8341 tctatacggt ccatagattt catcttatct cgtaaaattt gtctggactg ctcagagaga
     8401 ctatcatagg tgctatctgg tgaaactcct acttgattcc agagttgtgg cactgaatct
     8461 atatcggtaa tgatgacagg aataggattt ttttgaggga attcaaatag catataatta
     8521 ctgatagctt cctgaaaatc ctctctattc aaagcatcaa aggtcttgga agttatttct
     8581 gccagcatta aggcatattt ttctgattgg cgcaagtagt tattggtata ggctatatat
     8641 ttagcaaaga tacgaggtac atattcttgc tctcttttag cattacgaat tagaacctga
     8701 ctatagatgg caataacaac aaagagaacg agagtgctaa agattaaata taatcttaga
     8761 tactggaaat tattcctctt tcttggagag ttcccagaaa gagttgagtt cttcgagcga
     8821 tgcttcattg atattttctc cgttttgacg atattgctct tctatatact ggaatcgttt
     8881 agagaacttc cttgctgttt cttttaaggc actttctgca tcaagatgca atttacgggc
     8941 aagattcact atggtaaaga gtaaatctcc cacttcttct ttaatacgtg tctggtcttc
     9001 agtctctaaa gcagatttta gttctttggt ttcttcgtca atcttttcca gaataggtgg
     9061 tacatcatcc caatcaaaac caaccgatgc agctttttcc tgcattcttt gagcatatat
     9121 taaagcaggt aaggctcgtg gtatgccttc cagaacactt ttccgttcct gcttttctgc
     9181 tttctttagt cgttcccaat ttttcttcac ggtatcagca tcttttagtt ccagttcacc
     9241 aaagacatga ggatgacgac gcaccagttt gtcatttata tgatttaaga catctataat
     9301 attaaatgct cctgattccg aagcaagttg tgcctgcata acaatgtgca ataagaggtc
     9361 tcccaattct tccattaagg cgacattatc tccatcttct atggcttcta caacttcata
     9421 caattcttca accatattgg ggattaagct ttgtggtgtt tgtttaatat cccagggaca
     9481 acctgaaacg gggtctctaa gttgagcaat aatatccact aatttttgga actctttcat
     9541 tgtagttcac cttgcgtttt atttttggct aatctataca taaaagctaa ggttatccag
     9601 aacagcatac taacggaaaa ctgctggata aatatatcgg ttaaacaagc aaatagcaat
     9661 gctaaaacac cgatgaaaat tatagtacta aaataatctt tctgtctttt aactaaatgc
     9721 aggtagtatt ttctgacggt ggcaccgatg atataaaaat agcttaaaaa gccgaatatt
     9781 ccaagctcta cccaaatctt gagaaagata ttatgtggat gttcagcata gagaaaatca
     9841 agaggaataa ttgcagcata ccaatgtctc cagacatcaa ttcccatacc aaaccagggt
     9901 gccaatttaa tctgtcttat agcataaaac caggctatcg ctctgataac aatagaataa
     9961 tcaaaagtca agcccaattt tattcttgaa accatagaag agggaataat taaaggaatg
    10021 agcaaaagca caagcattcc tatatatcgg agttttttaa ccattataat aataagtata
    10081 agaccaaata atacggctaa aagagccata cgagtgtaag tatataacat acccaaaagg
    10141 ataatgattg ctccaataaa ccagaaatat ttattcttac tattttctgc ttccatgcct
    10201 aaggcaatag agaagaaaaa ggctaaagtg tagtatccat taatggtcat tgcagttatc
    10261 cacaaagaac ccagacgctg ttctggatgg gctaatgcca taaagacacc aattatacta
    10321 agaaatattc ctattattac aatagatttt atccagagta taaaatcttc tttttgaagt
    10381 ttggtattgc ctaataaaat gaaaagtatt aaaggaacta tagcagtagc agcaaaatat
    10441 tgggtggaat aataaaattc ccgtgctctt atcaatccgt agcaagaagt taataaaagg
    10501 acaatcaagg ctactggata gggtataact aaaggatggg aacgctgata ttcaaataca
    10561 aatgtaatta acaggataaa caatacgcaa tagaaactgg ttggtatagg tttgataaag
    10621 aaaagcgagc agaagattag gctatttctt acactgatac caaaaattag agagataact
    10681 gcccaagcca gaaagacaac gccaataaaa cctatttgga ctatctgcag tgtctggaga
    10741 aaatttgcta ctgccagaac agctaaagcg gcaaggagat aaatagattt ctcagccact
    10801 aactttagca tattcattac acccacttat aattctttct taatctcctg ccagcgttct
    10861 ttattcagtt ccagagcgtt ttttatcaga actatgatga tagacaccac aacagccagc
    10921 gcaaacgcaa tgatacacat tactgctctt tttggtctaa ctcttaaccc tgcttctctg
    10981 ggtgtatcca gtatatccag agttggtagg tctttcaatt cttccagttt agctgcttca
    11041 tattgcggat agagaaattc ataaaccttg gttttgattt ccaggttcat tttcaattga
    11101 gcatattgag ttgccagatc aggaagagag ccaatgtcaa tcagatatct tggttttaga
    11161 ggttcttttc ctgcttccaa ttgctttatc tgttttgcca aagcctcact tcggtctttt
    11221 agttccaaaa caataggaga attttcagca tagttctttc ttgccagttc cagttctata
    11281 tcagtactca ttttggtagc tataatatca gaataggatt taattaaaga ggaagtttga
    11341 gcttcaatat caacagcatt atgcctctgc tgaaagtcct ttaatgccag caagagggaa
    11401 tcaatttcgg cgcgagtttc attaactctt gcttccaaaa actcacgatt cattttgcca
    11461 cgggtaatct tttcttctct attatagata tccagttgct gcagataata atttactata
    11521 tcacgggaga gtttcttatc cttcgtatca gcgctaacag tcaccaaacc agtttctggg
    11581 tcactaccta ttttcatagt cttagtatga agcatcttca gagcactatc catagcttta
    11641 agtgaatcct tggtggttat tttgtaataa tctataagat tgaaatggcg aatcaccttt
    11701 tccgaaaatt gtcgggagtt catagctatt acagcactta tcgcattttg agaatttgtt
    11761 gttcccataa gaccggcagt tagaccactc aagccttcta tgttgaaagg aagagaggag
    11821 gtctggtctc ctataacata gaaagtagca gttgacctcc agatttgagg tgtgaccaga
    11881 ctgtaaataa ctgcagctac actaaccact gcaacaaata taataatgaa ttttcggttg
    11941 cggataatca accttattag ttcaaagaag tcaaattccc gtttttccat aatctcttag
    12001 cctcgtaagc tttggttttc ttttaatcta tttccgctca tacattacca tcctttactt
    12061 gattcagtaa tctcaggcac attatattct tttaaaacct ctggagcatt gatagttcct
    12121 tctttcgttt gataagtttc ccacaacgct atcattaaac gaggtgtggc tactccggaa
    12181 ccattgagag tatgaagata gccaagttta ccttgtttgt ccttatagcg tatatttgct
    12241 ctacgagcct ggaaatcctc aaaattacta actgacgata cttccagata ttttcccaga
    12301 cctggagccc agacttctat atcgtaagtt ttggctgaag caaaactcaa atcaccggta
    12361 catagttcta ccactctata atgaagattc aaagcttgca aaatctcttc tgcatcttct
    12421 ctaatctctt ctaaagcttg ataggaagta gaaggttcta cgaaatgaac taactctact
    12481 ttattgaatt gatgtaagcg ttgtaagcct ttggtttctt taccataaga gccagcttca
    12541 cgacggaaac aaggagtgta ggatacgaat tttatgggaa gtctatcttg tggcaatatc
    12601 tcatcggcaa aaaaattagt aactggcact tccgcagtag ggatgaggaa aaggtcatct
    12661 gcttcaattc ggtacatatc atcttccagt ttaggcaatt gtcctgttcc cgtcatagtt
    12721 ttacgtgtaa ccagcactgg aactgccagt tcagtgtaat tatgtttctg gatatggaag
    12781 tttaacataa aattaatcaa tgctctttcc caaattgcgc ctattcctgt ataaacaggg
    12841 aatccggaac cgctgatttt tgctcctcgg ggaaggtcca gaagcctatt tttctcagcc
    12901 aattctaaat ggtctaaagg tgtaaaagtg aagtttggct tttcacccca taactttatg
    12961 acccgattgg cagattcatc ttttccaatg ggaacatctg gatgagggat atttggtata
    13021 gttaacagca aattttctaa ctgagtattg gtttcagtaa gttgagcgtt taaagctctg
    13081 atttgctctg ctacttcact catttctgcc aggagttcac taacatcttc tccgtttctc
    13141 tttttcaata ctataatctg ggaaacctga tgttgtttac ttctcaactg gtcatattca
    13201 tattgcagtt ttcgtctgtt ctcatctaag gcaagaatag catccagatt agctttttca
    13261 tttttattaa ggatagcatt tcgcacctct tgaggatgag tgcgaatata tttcaaatca
    13321 atcatttatt tgctcccact attaatcaaa tctacggcaa tatctatcat tttacaaaaa
    13381 tcctctattt tcaaggaggc tccaccaatc agaccaccat caatatctgg ttcagagata
    13441 agtccgtaaa tattttctgg tttgatactg cctccataga gtatatgcac attttgagct
    13501 atttcttccg aataatgttc tttcaaccaa ttacgaatta aaagatgagc ttcctgagcc
    13561 tgattagcag tagctgtaag accagttcct atagcccata cgggttcata tgcgataatc
    13621 acatctttgc ctgtttgcaa ttcaatgccc tcaaaacaac catttaattg cgataagata
    13681 acttcttgcg ttttaccagc ttgcctttga ttgagagttt ctcccagaca aacaatgggt
    13741 atcatactct gttctctcaa gcgaagttgc ttatcacgca ccatagcatc agtttcagaa
    13801 tgatattgtc tgcgttctga atgtcctaca atgcaataat gtaatccaag agaactgagt
    13861 tgattagcag aaacttcgcc agtataggca ccttcaagat gagagctcac atcttgtgct
    13921 gctacttcca cgggtaatcc atttagaatt tttaaagctt tttctaaaaa aggataggct
    13981 ggagctatga ggggtaacac tctcatctgt ttatgagtta ccagatattg agcaagagca
    14041 ttacaaaaat cctccgtgct ttgggcatcc ttgtgcattt tccaatttcc tgcaatgatt
    14101 aactgtcgca tttgcctcat tcctattatt ttttctttaa taataacctt cctttgcaag
    14161 gtgtagatgc gtcaagaaaa ttgtatgaat ctaccagtca ggataaagag ttgaagtcat
    14221 attgcttata tatagatatt cttcttatga atgctggtta cagattgtta ggtagattca
    14281 ataagcacaa aacttgctat agtttcatct tttgcatgct aatcaggttg tagcataaaa
    14341 gaagaatatt tatttaacta tctctcaatc aaagtaaatg ggactttctt tgttagatta
    14401 ttgctggaaa aataacttta cagaataaga gaataattta aacggtagtt taggattaaa
    14461 tagtaaataa aaaggagaat tagatgctga aagtatttga atattttgaa ggtaaagtaa
    14521 agtctattgc ttttgaaaat tcacaaggta tggctacagt gggagtaatg gataagggtg
    14581 aatttgaatt tgttacttct acagttgaat atatgatggt aatatccggt attttaacag
    14641 tactcttacc gggttcaaaa gattggaaaa cttatggtaa gggcgaatcg tttttagttc
    14701 ctgcagacaa aaaatataga gttaaggttt cagagcagac tgcttattat tgtagatatg
    14761 aatagattgt tttctaaata atactaaaca aaagcaagga gagagtataa tttaaatctc
    14821 ttctatttac aatattttat ttcggagaaa tttacttccc agtgtttagg gtcatgattt
    14881 aaaagtaagg ttcccttctt ttcttttaag cgagagtaaa tatatagttt tgctttataa
    14941 gtttccgtgc gagaaaggtc gtaagcagag gttaccgcaa gagaaatatg aaagaaagta
    15001 gggataatat cagctggata tatataaaat tcggatgaag tatcaatttc tactatctga
    15061 ctgccaacag tatgacctcc agttttaatc aaagttacac cttccgctat atccagattg
    15121 ccttcaatca gttcatattg gttgctttgt tccagtagat ataattgatg ttcaaaatca
    15181 taggaggctt tatttaagtc atccggatgt tttgccattt cccattcaga tttttgaatc
    15241 cagtattttg ctcgggggaa agttagtctt tccttatctc caaagttagt tataactcca
    15301 ccacaatggt caaagtgaag atgagttaga atcacatcag taacatctat atctcgcaca
    15361 cctaattcgg cgagagaaac aggtagcata aaaggagaag gattatagat agaaatttgc
    15421 ttctccgaaa gacgattccc taagccagta tccaccagaa tgactcgttc tggtaaacaa
    15481 ataagtaata ggtttaatga cagcattctt ctatgaagtt catccgtctg aacttctttg
    15541 ccccatatag cacgcggtag aacaccaaag ctagctccac tatcggtcca gtattttcca
    15601 gcgtcaatac gattgacgat tatatccata aagttttcat ctcaaattac tttttttcgg
    15661 gctatgctga ccttgctgtc gttgttgtct actatgaata agagcctccg aagcagattg
    15721 atgagcagga aatataacca tcctcttttt agtcccttca ccgatagtta gagtgctcaa
    15781 gtctttttgt ttttctatat atttgtgaat tattcttcgt tctgatgcat tcaatggttc
    15841 taaggtatag ggttttcctt cggaacgcac tttctggaag atagaaagaa aaggtcttaa
    15901 aaactggact tgttgtcgtt gtctgtagcc atcaacatcc agataaatat gttcaattgc
    15961 actattttcc tcaaatactt tattcagtag atactgcatt gcatccagca tctttccgtt
    16021 ttttccgata agtctgcctg attcttcttc gctaatgatt tccagataga tggattttcc
    16081 ttccttatgg gtcttaattt ccttataaga aatatccatt ttgatgagta attcttttaa
    16141 aaaagaagca actctttctt ctatatcagg caagtaaaaa cgcaccatag cattttttct
    16201 gggaaggaga ccaaataaac cagaagaagg attttttatg atttcatatt taaattccca
    16261 atctttcagt ccatattctt tacgaaattc agcaatgagt tcttctactt ttgcaccact
    16321 tttctcaatg gtaatcattt aagtaattcc ttctttttca tatttttctt catataatac
    16381 atttgaatca cactgaggat attgaagact gtgtaataga gcaccaaacc agaaggaagg
    16441 ctcttaaata tgaagaacat aataatgggc attaaccagg tcatcatttt agtgcttcgt
    16501 tgcatagcct tttgttggtc atccattttg tctaaagttt ccttggaagg ttgagacatt
    16561 agactttgta gaaccataaa ccctgccata ataagcggaa gaatcaagta gggatcaggt
    16621 tcagaaagat cgttcaacca tccgaaaaat ctggcattac gcatatctat agtatagcga
    16681 agcacattgt aaagagcaat aaagatgggc atttgcagta gtaagggaaa acagccagag
    16741 aaaggattag cacctgcttc tttatacagt ttggaaagtt cctgctgcat tcgctggggg
    16801 tcatttttat atttcttttg taattcctgc acttgaggct gaatgctttg catcttcaga
    16861 tttgccttag tgctcttatt ggtcaaaggt tgaagtataa tcttgagcag gatactaaag
    16921 attatgatta ctataccata attaggaata aagctatgaa gaaattgtag caaccaggca
    16981 ataggattgg aaagccagcg cagccatcct ggacctcttt caggaatcaa ttccatcttg
    17041 gtatctatat agcttttcat aagattatag tctgcgggac cagcatagat gagaaaatgc
    17101 tgttcccaat tctgtgaggg actacgatca aaagaattga gaactatagc tggattacct
    17161 gttgtcggtg aaacctgggc aaaataattg gaggttaagg gatttcctgt ctcatttata
    17221 gctatggtaa aatatttggt ccttaaagct gcccatttaa atgcacttac tggaccttga
    17281 ggttgtttct tacgtacttt ggaaagacta tatttctgta attcgttctc ataaaaaaga
    17341 agcaatttgt aatcggtatc tttgagattg gatttaatct tttccgtatc tgctattcca
    17401 tctgaataat catattctat gccatatact gggaaagaac tactaaccgc tacatccatt
    17461 aaaataccgt atttatcatc taaggtgtaa acctttttga ccagaggatt agcctcatct
    17521 cctaaccaga acacaacact ggaatcaagc tgggtataat accagtttag gagctttaaa
    17581 tctttattct ggtctacatt taatcccaga agagaaattc cggctaatct accttgttct
    17641 ggaactaaat ctactggaac aggtttatct gcccagagat atttctttaa agatacagag
    17701 cttatgcttg cacctaaact actaaacttt actgtgaaat tgtcattttc taaagtgaga
    17761 gtttttatgc tatcaggata agaggcaagg ctatcccatt tagcaatagt ggcaggaaca
    17821 gccacaggag tgtttatagt atcagggaca gtttttgcca gactatcttg aacttgttca
    17881 ctttcagcag gaggtgtggg aggtttaaaa acatagacct gtaaaatagt gaacaggatt
    17941 aatacaatta aaagagatat gagagtgcgt ttatccatta atactcctca gggtagggga
    18001 tcgtaacctc ctttgcaaaa gggattacag cgtattaacc tccagatagt tagtcctaaa
    18061 gctttaaaaa agttatactt atggaaagct tgaagactat aactactgca ggaaggttca
    18121 aaacggcaaa ccttaggcaa gaggggtgat atcatctttt gatagatgtg tattagacct
    18181 attataacca gattaggtaa acggagaata gggattaata cttctaagaa agtcgttgag
    18241 ttagctcgtt cagttcttcg cataattgag tccaattcaa ttcacctgca cctggaagag
    18301 caataagatt aagtttaaat cctgatggta gttcattttt gtgctggtga aaccaggctt
    18361 taattctccg tttaagttta ttccgaatat gggcttttcc tatctttttt ccaatcgtga
    18421 tgccaactgc aaattcaaat ggcgaaacaa taaccggaac attaaaatga gaagttcgca
    18481 gaaagaggtc tggattgcga aactccaaat attccttatg cttcgttatc aaccgaagca
    18541 taatttacac acaaagggtc tttctccctt tagcacgacg acgagcaaga actttacgac
    18601 cattacgagt tgccatccgg ctacgaaaac catgagtgct ctttctggag cgatttgagg
    18661 gctgataagt ccttttcatt tatataactc cttatgtata aaacaatttt acttattttc
    18721 cttaatttta atccgctttt agatgtcaag tataaaaagc aatttacggc ataatcaggt
    18781 tgtaaaagtt tataatagct atcttatatc gttttactta gtttataagt ctaaaaaaag
    18841 gaagctatca attagagaga tttcattaaa taatgcagga atatacctag ctggaattta
    18901 acgattgtgt aaaaacattg acaaaactga taataggatt tgtggggata acataaaaag
    18961 ttgggtaagg aaataatttt aagactaaaa aggaaaagaa tgaaacaaga atttatccgc
    19021 aatttctgca taattgcgca tattgaccat ggaaaatcta ctttggcaga ccgctttttg
    19081 gaatacacgc atttagtgaa taaagtggaa gtagaccagg tgctggatag tatggacttg
    19141 gaaagagaaa aagggataac tatcaagagt cattgtgttc gtatgataca cagctataat
    19201 agtcaggaat atgtgttaaa tttaatagat acacctggtc atgtagattt ttcttatgaa
    19261 gtttcacggg cactggcatc atgtgaaggt gccatacttc tggtggatgc ttctcagggt
    19321 atagaagcgc agacattaag caatctctat ttggcgttgg ataatgattt agaaatttta
    19381 ccagtggtta ataagataga tttgcccaaa gctgatgtag aaggtactat ccaagattta
    19441 tctgacctgt taggatgttt accagaagag attatgaagg taagtgcaaa aaatggagaa
    19501 ggggtcaaag atttactgga tgctatctgt gaaaaacttc ctgcacccca gggaaatatt
    19561 gatgctccac ctaaagcact gatatttgat tctttctttg atatgtttcg gggggtagtg
    19621 gtcttagttc gtttatttga tggaagactt aggaaaggag aacggattaa gctttttgcc
    19681 acagagcatg aatatgatat agaagaaatt ggatatctgg gtttgaaatt gtctccccaa
    19741 gaggagttaa ctgcaggaga agtgggctat attattgcca atatcaaaga agtagcagat
    19801 gctcgggtgg gagatactat aactctggct aaaggtggtt gtgaagaacc attacctggt
    19861 tttaaagaac ctaaaccgat ggtctactcc agcatttttc ctatcaatag cgaagattat
    19921 gaaaatctgg tagaagcaat ggcaaagctt aaattaaatg atgcttccct ggtttatgaa
    19981 aaagaaaatt ctgccgcact tggttatggg tttcgctgtg gttttttagg aatgttacat
    20041 ctggaaatag tcaaagaacg tctattacga gagtataatg tgcctattat tgccactact
    20101 ccaagtgttg gatttctaat taaattaaaa aatggtcaag aagtattgat taataatccc
    20161 gcggattttc ctgatccgac tctcattgaa actattaaag aaccctatat ggaaactgaa
    20221 atcatagtgc ctactgaata tattgggaat gttatgaaat tggctcagga acgacgaggt
    20281 attcaaaaag atattcaata tctggatgaa aagagagttg ctctgcatta tgagatgcct
    20341 ttaatagaga taatctttga tttttatgat aaacttaaga cggttagtag gggttatgca
    20401 tctctggatt atacttttct ggaatatcgt ccttctgagg tagttaaagt ggatatcttg
    20461 attaatggag agaaggtaga tgcaatgagc tttatctgtc atagagataa ggcacatagc
    20521 tggggaaaga gtgtaacaga tactctggcg gaagtaatac cgcgtcatct tttcaagatt
    20581 gcacttcaag cagcaattgg tggcacaatt attgcccgga gcactataaa tcctttgcgc
    20641 aaggatgtat tagcaaaatg ttatggtgga gatgtgacca ggaagcgtaa actgctggag
    20701 aaacagaaag aagggaaaaa gaagatgaaa gagataggct ctgtttcggt accacaagaa
    20761 gcgtttctgg cagttcttaa agctgacaga gattagtttt tggtttatga aaaagttctg
    20821 gcttatcttt ctcgctttag gtgttcttag ctttaattgg gctattatag tagagaagat
    20881 aagttttcat acaaacttct ccattgatga agataagctg ttgcaggctt caggtctgga
    20941 gattggctcg gaatttgtac ccgaagaaat caatagggct acaatctctt tgcaaacctg
    21001 gttgcaaaat caagggcatc catttgtcca ggtgtcctat ccagatttga taccactttc
    21061 agaaacaggt atggaattat cctttcacat agaagaagtt attccagcgg agcattgctt
    21121 cttacacttt tctgggttaa ggtatttttc tgaggcaaaa ttacgagatt tattgcttat
    21181 ttccgataaa aaggaagtga gtgtaaatga gctaacccga ttgatggaaa atatcttaag
    21241 ggaatatcat cagcgtggct tcctttttgc agcagtgcag ttggattctt tagtgttaaa
    21301 taaccattta gaagcataca tcagcattca agaaggaaaa ccgtttaaac ctgaaattta
    21361 ttttattcag ggtaataaat atactaaaga taaaacctta attaaactct cgggattaaa
    21421 tgagctaaaa gtgattactc ctgaagcaat tgaaacagcc caaaaaaata tcctgtccaa
    21481 aagttatatt caatcctgtc atatagaacc ccttgatccg ggtagcatac ttatcaaagt
    21541 ggaagaagga aagatgactt atctggaagg gataatgggc ttaaataata ggaacgaaaa
    21601 aacagaactg acgggttatc taaacttgaa atttctgaat ctctggggaa gtgaccgttc
    21661 tatcgctctt aactggcgtc aaaatcccag ttccagcaat ttagaattcg cttatcatga
    21721 atctggaccg attaatatac ctttatcagg tgaccttgct ctttcacgta tggtgcaaga
    21781 ctccacctgg ataaaatcat ctgtacagac cgatatttat tcctaccatg ctaagcaaaa
    21841 gtatggaatt gaattagcct cggaagagat atctcctggt agcagaagac ctattttagt
    21901 agaaaaatct tccgcgaata gcattggagc tttttggcag ttagatacca gaaatcaaat
    21961 atttaatcca actcggggaa ttgagatagg tataaattac acacttaaat acctaacttc
    22021 agaaaggcaa tggataaact cactggaaac atactatact caatatttaa atatatccag
    22081 acgttttaat gcctctatgg ggtttcatct atgcagtttg gatgatagca cttctgccga
    22141 atatctatgg tatcctatgg gtggatttaa ttctttaaga ggatatcaag aagatgaatt
    22201 tatcagctgg cgtttagctt ggtccaatct ggaaatacat tatttaataa atccagatgc
    22261 tatgttatac tgtttttttg attatgggat atatgcaaga acccaaactt ctattaatgc
    22321 aaaattattt gctccaggat tgggtttgag ggttagaact cgtttaggaa ttctaaggat
    22381 ggaatatggt ttaggttata gagaggatgg atttccctca cttgcttctg gaatggttca
    22441 tgctggcata gaaacaagtt tttagaaaaa tcttgacaaa ataaactata ttttattgtg
    22501 tgatattaat aggtcagtat atagaagtgc ttaaggactg tgagaaaggt tagtaaagag
    22561 ttttaggctt tgagcctttc tatttttatt gagctaggca aggctttaat ttgccaatta
    22621 agtaaaaaaa ttaaccctaa aaataagatt tcactaaatg gaggaatcaa aatgaaaaaa
    22681 atcgtcgtaa tagctcttat cgctacaatg ttaatcggta tgctggtttt ctctgcatgc
    22741 aagagcaaag aaacccaaga gcaaggtatt gatactactg cggttataga tactactcaa
    22801 gaagttagtc ctgatactac agtagttcca atgcctgaat aatttccgca agttcagtaa
    22861 ccctgaatat aaatcagcgt tactaatagc gcagtcctta aaaaaacggg cataaaatct
    22921 gatgcccgtt tttttatttt tgattctaat atctgaactg ataacaaaaa aggtgggaaa
    22981 tactcccacc ttttttatta tatgccaatt agtctaaatt atgtgacctt tgccaactcc
    23041 acgaacttta caacccaatt gtttatattt attaataact tcatcgttta gaaaattaga
    23101 acaatcaaca atcagtggtt tggttccctg acacatagca acaacttctg cagggtctaa
    23161 atgtttatac tggttgtgtc ccacagcaaa aattactaca tcagcttgag gcaagacaga
    23221 gttcaggtct ttttccacct tggcatcttc caattcgggc caaacttcca catagggatc
    23281 atgagtgtaa actaaagcaa actccttccg aagaaattct accagtgttt tggagggtga
    23341 atggcgagta tcagctacat tttccagata ggacacaccc agtactgcta ctttcacatc
    23401 tcggagagca ggaatttctt ttttaataat gtctatggta tgtagtggca tagtatcgtt
    23461 aatattgaca cttctaattg ccatttccaa aaagtcatta atctcaaaaa gagtttgcat
    23521 agcccaatta gccagaactg ggtccttagt taaacagtat ccaccaacac caagactggg
    23581 acggcacata ttatcgtgcg tacctttacg cttgcgaata gcttctctaa ccttgaacaa
    23641 atctactcca atctgttctg catagcgtgc ccattccaga gttaaagcaa tattgactgc
    23701 acgataggtg ttctccatcg tttttgccaa ttctgaggca ttagtgttgt ccagttgagt
    23761 taaaggatat tctttaacat tgaggacatt ggagagaaat tcacgacaga gttccgcact
    23821 ttcaggattg accccagagt aaactctcca gaaatcacgg attgatgcca cataatttgc
    23881 accaggcata actctttcat aagaatgagc aattaagggt ggatgaagag caatgtctat
    23941 tccgcgctta gtaaattcct cttccagaat aggttttacc actttttcac aggttcctgg
    24001 gggaactgtg gtttctacca taaccagaca atttggacga atacgttccc ctaaagtgcg
    24061 aataccttca cgaaaagcta aaaggtcaca atatcctttt tctgcttcac caaatgatgg
    24121 tttagtggca tctaactgaa tatctaccac tacaatatct gctaaagaat aaacgaattc
    24181 ttcactcgta gctcggaaat tcttcttttc aattactgtc cttttgaata tctgaggaat
    24241 ttccgggtca gaagaattaa ccggaggaac gcctgagttt atcataggaa ctttccaata
    24301 agagcgttta gagggacgtt gatgtccatg cacataataa tagggattcc catcttcatc
    24361 tgtggcatcg gctactactg cagccataac gcaacctaca aaacctaaac cctgaactac
    24421 cactattttt ctaccaagtt tccgctgttc ttctgttatt tttaacaggg tttcgcgttc
    24481 cttggcatta tctagttctg tgggtaaagg atatcctgtt ccgtcagggg caagtgaaac
    24541 ttcaatcatt gacgagtgtc tcctttataa aatttaagaa tgtataatat tttggataaa
    24601 cttgttctgt caagctttat ctgaccttgc tatttaactt tctataataa agaacagggt
    24661 atataaagga gatactctta agttactatt aattatcaat aaataataat ggctacagta
    24721 aacaatttaa tgggtaacaa atattaagct tcatcagcgg ggaagggtat atattcttga
    24781 gatatgggcg ctgcaggtgc cgttttcagc ttttggataa tatggtagac aaagataaag
    24841 gatagaatag tggtaaaagc atcacccata ggcatagcat agaccagtcc attaaatccg
    24901 aagaatttat tcattattaa cactgcaggg atatagatta atccctggcg tgacagagaa
    24961 acaattagag caggaagtgc ttttcccata gcttgaaggc tattcataaa aatcataccc
    25021 acactggcaa aaggaatagc ccagacatat gcttctaaga cttttttacc tatgtccata
    25081 atctcctcat tgtggataaa tattccaatt aagccacgcg ggaaaagaat aaagattaaa
    25141 gtaaagatag cgcttaatcc cagattaatg ataacagcac tgcgtaatgc ttttttcatt
    25201 ctggaataat tacctgctcc atagttaaac ccaatcagtg attgaacacc catagccatc
    25261 ccaataaaga taaatagtgg gattgaaaac actctgaaag cgaccccaag agatgccaca
    25321 gatgcatctc cataagagga agcaagtctg taagttatag catttcctac actcatcatt
    25381 atctgactta aagaggcagg aataccaata tagagtattt ccttaaatat attccatttg
    25441 aagtgtagat attttttccc aggaattgca actgcatgag tggaatgata ataccagata
    25501 taatacaaca ttgccagacc ctgtccaata accgtggcaa tagcagctcc tgatacaccc
    25561 atatttaatg caaagataaa tatagggtcc aaaataatat tagcaccagt cccaataaat
    25621 agtccataca tagaatattt agctccacct tcacctcgta tcatttgaac catagtaaat
    25681 tttacaaaga ctatggggct acctaaaaac atccagaaca tatatttact ggctggaact
    25741 atggttgcag cactggctcc tacagccttt aaggtataag gcatagttaa tatcccaact
    25801 atagacacta taatagatat aatgaaggta ctaaatattg agatggtggc agtttccttt
    25861 gcctgataat aatctttttt gcctaaaagc cgggaaagat aacttgcacc cccaactcca
    25921 aatataccag caatagcagc ttgcatagta aataaaggca gagctataga aactcctgcc
    25981 atagcattag gatcttttaa ttggcctata aaaaaggtgt ctgttaggtt atataatata
    26041 tttatcagca tacttaacat actgggtaga gctaagctaa gtatggcttg aaatataggc
    26101 atttcctcta atatgaatat attattatgt ttctttgctt tctccatttt taattctccg
    26161 agtacaaaat tataaaggga ttttatttgt caacttctaa atattaattt ataaaatgag
    26221 ttaaaaccct tatctaacca tattataaag agttaatcct ttatttgtct tattttagta
    26281 taatttgtag cgtttacctc catttataat atatttaatc tcagtaaatt acattattta
    26341 ttatatctta tttcacctaa atatagtgcc caacaaataa taatgatagt aaattaatat
    26401 aatattatta aggcaaatat taaaaattaa aagtaatgaa tattatttgg tgtaactaat
    26461 tcaaacagta tgtattagca aactatccag acacttatct cttaattgcc tgtctcttag
    26521 ctatttacat ataaaagagc caagggagag gcaggggatt tgaagggaaa atagtagtta
    26581 caagatagag atgattaaac gggacaaaaa taaaggataa ttatttattt attaataaga
    26641 taaaacaatt tcatataatc aaataagagg atatagtcaa taaattaact ttatagctga
    26701 tagagaacaa aaagataacg attattcagc ggtcaataat atcattttgt aatgatgtat
    26761 atttattaaa ttatgctaaa ataggtggga aagcagaaaa tgtagctata aactatggat
    26821 atttatttag atgctccaga aacgtaaaga tttatgagcc ataatttcat gcgtcatttt
    26881 aggcgtaata ggttcatatt tgaaggtata agttatgcga tattgcctgg gaagaatcca
    26941 acgtaaggaa gtatcatgat aaattaaaaa cttagttgat tggaggtcag tagcttcttg
    27001 cagggtgaat tgatagtcca agaacattct taatccgagg tatttactca tagatataga
    27061 gaggttattg agcagtattg atgagctgaa ctggacgata tcattcataa actggttcat
    27121 atcggtgtat tctgccaagg gataagggct ggtggtatat tcattaaaga gattttggat
    27181 aaaaccgatt ttaatgctaa agttatccaa tttaagccat cttcggagat tattttctaa
    27241 aggatagatg actggcgaaa taaatgaata atataagttt tcagaaatca tactaactgc
    27301 ttcttgacgc aagatgtttt gttcttcagg tgtattctgg cttgtttttc cccttagcat
    27361 agtttctctc tggataatgc tggcgatagt aatgtcattt tcattgtcac tgctaagagc
    27421 gaagattagt ctttgtaaga gtggttttga aacatccggg tccattccca catccagagt
    27481 aacgatagtt ccatcaggag aacgataatt aaatcttcct ttaatatcaa ttttattttg
    27541 cgattccagg atttccaggg caattgtttc agtttggaag atagtgccga agaaatccag
    27601 tgtaccctgc tcagaggaaa tacttgcttc tgctactttc catttatgtc catcgtaaat
    27661 tagatgcaga aagctgcctg gctgtaggta aaagttagtt ggataggtgg catatttatt
    27721 attttcgccc agacgaatca taagatcaag tgtgaaaggc aaaggaacag gttccttagt
    27781 tttttcttca tctttaatga tagagctacg gatattgtag ataaggttta acaggttatt
    27841 agtatttggt ggaaagacaa tatttgcatt atagaccagg atttcgccgg aaatggtcat
    27901 atcttcaaag ggtccataga tggtagcata ttcagtattt tgcccacgaa tgataacttt
    27961 actaagactc aaaggtgtag ttatttcagg gatataaata agagctccgg gttcatctat
    28021 ttttaaagcc agagttccca ggtccagaaa tccgacatta agatgaatac tttcatcgta
    28081 atcaaagcta ttttttaccg tcaatttacc ttcacccata tagcaggtaa aattctccaa
    28141 gaaaatcaga ttatcctgaa ttttggctga tatatttata ttacgaatag gttctgcctg
    28201 gtcttgaagt aatatagagc catcgttaat agagaaattt ccatttttga ccataagttg
    28261 gtcttcaaag gtttgtaaac tgcaatctaa atgggctttg ccgcgggcat ttttaaccag
    28321 cgggacattt ttatccagcc attcaaatag taagccatcc actttaagct gtaaggtgtg
    28381 gttaccttca aaataggtat tagtgaggag attgtaatct atagcacctg aaccagagac
    28441 ggttcctaac tcgttgcact gaacatataa agtgtccaca atcagcacat ttttagtctg
    28501 agatagcata atctggatat catccaattg agcaatatca gggactgcaa gttgattgga
    28561 tttaacatta acatccagaa tgatttctcg gtcgggtgat agcgtatcta tgataaataa
    28621 acccacattt ccatagacat tcccttccac tggaggagaa tacatcaaat ctctcatatt
    28681 caaatctgga attattgcct gaagacgtat atccagatta tcaacaatat ttagtgaacc
    28741 tgaaagagaa gctaaagctt tggaaggatt attaatagtg ccatttaatt gaacttgctg
    28801 taagggtcct tgaaagttta aattacttga tactggttgg agacctggta taattaaatc
    28861 attaaatttc aagtttgcat taagaggttt ttctttatct gaatcatatt caagcgatag
    28921 gttaagattc tggaattcag gaagattaag tgctggaaaa tagtttttaa tatcttgata
    28981 atccacattc tgcacttcaa gttgcagata gggatttttc acatttttaa catctacctg
    29041 tccccgagca taaatctggt cgtttatttt taggcgttga atatcaataa ccgtgttttt
    29101 aagattagca ttaagttcca gattaactgt attattgtcc agttctgaag gagcgtccaa
    29161 aattattgtt ccttcattat tttttaggtc gtaggaggca atcaaatcta tattggattg
    29221 gaggtctaca tttgatttca gttcaagttc aagtttggag gcaaggacaa gtttgtttcc
    29281 tgtaaggaaa gcttgaatat caccagtaat ttcaggtgca atatcggtaa tagctttatt
    29341 agcataaaga cgggagatat ctaaatcctt acatttggca tccagaagaa aatttttgtc
    29401 ttgaatatca ccaataagag aaaggttaaa accttgagaa ctgataagag ctacatcaat
    29461 ataatagttt ttctgagatt cagagattag tggaagaaaa ttggcataac cgtttattgc
    29521 atccagtttt atttcatttt gagcatagga tagtttattt atttgcgctt ttacttcagg
    29581 cacagaagtt gcaaaattga gttcaaaatc cgcgtcagca gtgattttca tttcttgggg
    29641 atgatgattt ataggagtag tgttcaaaaa gccagttaat tgacggtttt ctaaagaata
    29701 atctccatat agattgatat tttggttttg ccagataaga ttatttagag taaatgatgc
    29761 tatctggtca agatagaatg cttggaggtg gaggttttgg aagtcctgat tagcataaga
    29821 aactttttcc gatgcggcat tcaaagttaa ttgaggttcc gagaaatatc catctgcaga
    29881 taaagatgca tccacaatcc cttgcagttc gggagttagc atcgccaggt ctaattttag
    29941 ttttaactca ctaggctcca gacctatttt atctgttatc cccgtaacaa ctactcttcc
    30001 tcctatggcg ctggttccaa ccgtgctggg tgaaagttct accactaatc tatcctggtt
    30061 tcccagaata tgaagtgatg gaatgtacaa tggaaactgg gaagctaaca acacttcagt
    30121 ttggtgcaaa aaagtttttg tttcaaattc cagttttgca tctttgttct tctgcgaagc
    30181 tttaagagtg aggtctattt cagtatcaag attggtaaat tgaggatgat taatatactg
    30241 gggatggtaa tttctcaatt cagtttgtaa agagcttaag ctacctttat ccagaacacc
    30301 attagcacgg atagtgccat cttttgcact ttgaccagaa agtttaaagt tagagacatc
    30361 agtgtttacc atattcaagc taatatggtc aaattgttct cttatgctta aggtgctatc
    30421 agttcctatt ttaagcggat aatctaaagc aagaagaaag cgggcattat ttacggttgc
    30481 ttgattaaaa tagaggcaaa ggtctggaat gaccagtttc ttttttggtt tcttgactaa
    30541 tggaggataa gaatagctaa cttctgcgcc atctacctca actttgtcca ggatattgcg
    30601 tatcttaaat cccgaaaaga tgaatttaag aagattatat ctacaatgag cattatctac
    30661 agtgaatttg aaagtgccat cagtggaagc aaattgtatg ttttgagcaa agatttgtcg
    30721 ctcaccgata gagagatttc ctatctgcat ttgaccttta agagccttac ctacttcatg
    30781 ttcaatgagg ttttttactt ttccttgcac atctaaaaga taccaggaaa caaaaaacag
    30841 gacattgatt aacaataaaa tcaggataag ggtcaaaaat acacgggaat gtttcattgt
    30901 tctaaaaaag ctgaccgtct cttaattcat aggacttatg agccaatttt gccaattctg
    30961 ggtcatgggt gacaatcacg aaagtttgtc ccaattcacg gtttaatctc tgaaatagat
    31021 tccatatttc ggtgctatgt tgaggatcca gattacctgt tggttcatca gccaaaacta
    31081 tatgcggctg attgattaaa gctcgggcga gagctaatct ttgttgttct ccaccactca
    31141 attgattggg ataatgccaa gctcggtctg ataactccag caaacgaagc agttcatcag
    31201 ctttttgaaa agctacggat ttattagctc cagcaattaa cataggcaag accacatttt
    31261 cccgggcatt aaggtcatca atcagataat gaaattgaaa aacaaatcca atttgctgat
    31321 ttcggatttg attagcctga ggatgatgag tggaaacttc cacacctttg atgaatactt
    31381 ttccgctatc cggctcatct aacaaaccaa gaatgtgtaa aagtgtgctt tttccgcaac
    31441 cggatttacc ggtgatacag acaaaatctc cacattctac ttccaggtta gttcccctta
    31501 gcacccgaat atcatttccg atatcagcgt aggtcttgta taatgaatat ccagctaagc
    31561 aaagcaaatg tcattctcct tatggattac gtaaaaggtc tataacttgc atcttatcta
    31621 tctttcttaa tggtatttgc acacaaagaa ataccagaat agaggctaag atgaagatta
    31681 aaatctgatt taaaggggct atatagagag ctaatttatc aataaaataa acttcaccct
    31741 taagctgata gatattttga tgaactatta gccaggaggc aataaatccg agaattagcc
    31801 ctaaaataat tgagataaga caaagtagag taacttgata tcctaagata tgacggatag
    31861 tagattttgg agctcccaga gctttgagaa cagcaatttc attcttttta tcataaatct
    31921 gggtggagat tccgctaata atattgattc ctgcgataat caccagaaag aaaaagacga
    31981 taaagattag ccatttttgc atcttgacca gacgtaaaag tgttgtgttt gttgttggta
    32041 atgctaccaa attactatcc attattttat catattcttc tgcatagaga ttaatgtcat
    32101 cggcataatt attttttaat ctgatttcca gacaactgaa cccatcctta gctccagaaa
    32161 gaattcttgc atcctctaag gttccaataa tgagtgtcct atcatattca taaaagccag
    32221 aatggtaaat acccgtgatt ataagaggag tttccacagg atacattccc aaaggagtta
    32281 tcttatccaa acgaggatag aaaactttga gagtatcacc cagagaaagt ccaagttctt
    32341 ttgccagata ggttcctaca atgatattac ctttagctaa ttgcgcagtt ccttccttca
    32401 catatttagc ataattaacc ttactggcat aatttacatc ataggcatta aatatagttc
    32461 ctcttgcttt tgtgccattg cttgccatca gatttgaagt taaaagtggg gttaccgagt
    32521 caatttctgg tctgctattt agttcttgta aggtagatga aatcagggta tcagataata
    32581 attcgccatt tatgtgttca attcggatat gagcaaatga atccagaagc acagatttga
    32641 gagcatgttc atagccttca aataggttca aggcagtaga aagaattccc acagagataa
    32701 ttatacccag caccataaac agaaagctaa aacgccatag atgttttttg ggagcttgaa
    32761 tatagcgttc aaggaataac ttttcagctg gattcaaggt ctaatccctc tttattctgg
    32821 atattgacag ttttttctac tgatacgggt aaaggacgca aagggacact acgatttacg
    32881 ataagttctg agattaatcc cagggaaaca aattgtaagc ctccaagtat cagggtgata
    32941 gcaaggaaaa gtaagggtct attgcttaga ggttgattat agaaaagttt cagaacagtc
    33001 aggtagattg ctataattgt accgattagg gttgctatca aaccgactct tccgaataaa
    33061 taaagtggac ttttagcata ttgagttacc atcttaaccg ttagaaggtc aaaaaaacca
    33121 cgtaaataac gttccattcc atatttagag tgtccataaa ttcgtttacg atgttcaacc
    33181 ggtatctcag ttattttgta acccagagaa tgtgccagag caggaatata gcgatgcatt
    33241 tctccataga ggctaagttc tttaaccact tctctacggt aagccttaaa accacaatta
    33301 aagtccttaa gtttaagatg gaaagcgcga gcagtgacaa aattgaaaaa tcgtgaaggt
    33361 aaaaccttat gccaggggtc atttcttttt tgtttccagc cagaaacaag gtcccaaccc
    33421 tcagctattt tatccagaaa ctttggaatc tctttagggt tgtcctgcag gtccgcatcc
    33481 agagtaaaaa ctatctcacc ttgcgccaga ttaaaaccat actgtaaagc agcagctttt
    33541 ccgaagttac gacgaaactt aactacttta atgttcgggt cttgacaagc cagctcttcc
    33601 ataatggcaa agctatggtc ttgacttccg tcatctatga agattatttc ataattatga
    33661 ggagcacaat tagctatgat ttcatcggtt aactgtttta aagaatcttg ctcattgtat
    33721 actggaatca cgaaagataa aagcatattt gtccttctta aataaaaata ccggctaaag
    33781 tcataagtat agctgataaa aagggaattt ttatattgtc atcaatacga atcctacttg
    33841 cctctgccag agtagcacct aaagcaccaa taagagcaat tacagggtct gctttaaagc
    33901 aaagagcaaa gacaaaacat acaataaaac aagcaagact tccttctaag gttttattac
    33961 tatttttata ttttctcttg cccaagttca gtcctaccag tgcagctgaa gtatccccca
    34021 gtgaaagaaa acatagagca aaaaaagtga cttctggagg aaagaaagcg atacaaatta
    34081 cggaagaaaa gaaaagataa gtagctccag tgaaatcata cagctcttta tgtcttaata
    34141 ccataccgaa aatcagccag aaagtttttc ggaaactctt ttgccataaa cgataaaact
    34201 ctacgaccag cattagaatg aatacaacca gcataatgga aaacataaga ttgcgattgt
    34261 aattcagtac ataacgatac cccaaaggaa taataagtga actgagatgg atggtcttgc
    34321 gtaatgattc cttgaatttc atcgttcgtt aatccgatag aatccttaag caatggaaga
    34381 tgtttcggta aatgatagta cctttaagac ccttggagaa atccaactaa ctaaaacatc
    34441 tccctttatg ttatcaggga agttaaaacc attaatatcc acttgaaatt tactctgcaa
    34501 ctccattaaa gtggtatctt taatcaaaaa acagggttca ggatggtctt gtccataagg
    34561 gagtaataat tctaaggata accaattttg ctcatttagc tcctctaaac tcaccactgc
    34621 atcaggttca atctttagat tagtaactgg ttctgggcaa ttttcatcta aatactggtg
    34681 gaaacattcc aaaaaagagt catatttttc aggttcaaga gaaaaaccag ctgctttagc
    34741 atgccctcca tattgtttta aatgctcttt acagaaagta aaagcttcca ccaggttaaa
    34801 accctcatta catctacctt cgcacacaat ttctgaatta tgtaacttca taataagggt
    34861 tggaacacca aactttcctg caatgtagga agcagcagca cccagaagat gataagggat
    34921 aataccctca tcgtcaaaat atataaaagc agaaccttca aaaccagtgg tgagcgtatc
    34981 cagaaagttg taaacttttg ccagttctgt ttccgactga ttctttaagg aatctaaatg
    35041 gtagaacagt ttttctttct caccattagt ttcaagcaaa aagcgtaagg cagcattttc
    35101 accattttcc tctctaccat tggaaaggag tcttaccaga tattgaatca gttcatagat
    35161 atctgaataa gtttctatgg gaccataata gggcactaga aattcaaaag tagagtcctt
    35221 aagttcttgc cagtgttgga atgcatagcg gacaattata cgatttacat cagtcatagg
    35281 cactctatct gcaatagaac ctacagctgt ccaaaaatag tagtctggag ttatgtcata
    35341 ttccagttct ctgcctaaat aacgaatcag cattagcact actcctactc cagcgagcat
    35401 tttataggga tatgtacttg agggcaaatg tggattgacg atggtgtagg cattgggaat
    35461 tttatcgggt tggatgagat gatggtcagt aataagtgtt tcacaaccaa tctggtttag
    35521 cttttctact ccttcaaaac tggcgatgcc attatcaact gtaatgacca gatgataatt
    35581 accttctttt acaaaattta cgagactatc ttgaatacca taaggttcaa cattacgatt
    35641 gggaatgaaa tatctatgat tctgaaaacc gcaagtattg aagaattggt aaagtatata
    35701 agcgctggta atgccatcgg gatcgtcatg tccgaatata acgagaggct cgttctcgta
    35761 catagctttt ttgatgcggg ctgcacactt ttctatgtta gccagtagag cttcgtcagg
    35821 aagagcctct aaaccagctt ccaaactttc tatagatagc cctctttttt gaataatttt
    35881 ctctaataaa cttgggggtt ttccctcgga taaattttct accataaagt cgtttcttcc
    35941 ttttcaggaa tatctcttgg cactgttcgg tctataggtt tggagaaaat atcaaaacca
    36001 gtaaagccaa tcataaaaag aaatgaattg tccaggagtc tatttttctc cagattacct
    36061 cgctggctat attgaaatcc caagtcaatg cgatttacac taactggtag aggtagagat
    36121 attcctaaag atagagcaag ttcattgaca tctgcttcat taacctgaaa tgggagatga
    36181 cgataagaga cacctgttcg cataggaaac ttcaacattc ccttatgttc atctgaagca
    36241 gagggctcat aagcaaaacc caatccagct ttccagctat cggtatattt gttttcatct
    36301 atagcttccc aagtctcata atgtaaatca caggcaactt taaactgaga taagggaagt
    36361 gctgtcacac tcaaagagta ttgagaagga atctgtaagg tataagatat aggttcttca
    36421 gtttgatgga tagaggaacg aacctcttct cctttcaatt ttgtagtcat agtataatga
    36481 gcaccaagag caagtttatc attatgccag agataaccaa gcgtaaaagt gggattctta
    36541 aagtctctac tgagcttttc tcgggtatta aataatccga aattaccttc ttgcttaaaa
    36601 tctctaatat catgcccaaa aaagtaattt ccgcttaccc caaagctatt attcttccat
    36661 ctataactat aaattaggtc tgcccgatag atataacggt ctatggattg tttttctact
    36721 acttcagtgc cgtcttccaa taaaaaagta tgttcattgg aaaccaatcc tgaaacataa
    36781 ggattaaact ggaaaccaaa ccgatgaagt ttaaggggga aacttaagct aaaaagaggg
    36841 aaatccagcg agttatccag gaagcttttt gtattgcctt gttcatcttt agagcgatag
    36901 gaattgtagc ctggtataat tccagttgaa aagaagcagc gattatccgg attattcata
    36961 gccgggttgg caaagccatt gtttacccgg aagatatcgc tggcacctgt atcacccatt
    37021 cccaggctat aaatatcggt gccatagtat tgcacaggat atccatcata ggagaaaatg
    37081 gaattaccag cacttaaggt aaaagttaaa atgcctaatg ctgatataag aaagatgatt
    37141 ttcttcactt tttattttgt ccttttttaa tcttagttaa agtctttcct gtggaaggag
    37201 ccataaagtt cttacgggac ttggcgattt ttgccgtaac acttgtttta tgactgctct
    37261 ttcctcttgg aggttttata ggatcaggtg gaattctgaa aaagcgaaaa gcgttgttaa
    37321 aggaatgttc tgccacttca ttaggtgtga ttcctctaat atcagcaatc ttttcagcta
    37381 caagatgcag gtaaagagga ctatttcgct gtcctctatg aggatgtgga gttaggtagg
    37441 gacaatctgt ttcaatcata aactggtcta agggcatcat tctaattaca tcgtctaacc
    37501 tggaattggg ataggttata gaaccagtaa aagatatcat ccaaccttca tcaagcacct
    37561 gttgagcaaa gattaagtca ccagaaaagc aatgaaaaac cacatccttc gcatttgcct
    37621 gtttcagaat tttaaagcat tcttgatgag catcccggtc gtgaacgatg atgggtaaat
    37681 cgtaatccac tgctaaaaga acttgattgg agaagacttc tctttgaacg gaataagggg
    37741 caagattgcg gaaaaaatcc agtccaattt caccgacagc taaaactttc ttttctctcg
    37801 caagatactt tactacttca gcatcaaaat cggttgcctc atgaggatga aatcctaccg
    37861 tggaaaagat atgaaggtgc ttctttgcta attccaaagc gttcagtgag gtttcattat
    37921 taaaacctac attgatgata tattcaatac cgctattgaa gcattcctga atcaatgact
    37981 cgcggtctga gtcaaagtcg ggtaaatcaa gatgagcgtg agtctcaaat agtctcattt
    38041 tttgttaatg tattcccatt atatttttaa ttcaagaaaa atgtagattg attttcttgt
    38101 caatttaaaa gaatttagcc cgtttatacg attcttgtcc tgttttccct tattcttcag
    38161 tagatattat ttaggttatc ttatcttatt ataaataatc aaattagggc ttgactttgt
    38221 tcttgccaag ccctaattat ttatctatta ttgtagttta tttccagaat gtggttttaa
    38281 ctctgagcta atttcttata ctgaagatat cgctctttga agaattcttc tgcctcattt
    38341 tgtagcattt cagaacgttc gggtgcaata tcttttaatg cagcataacg tctttcactt
    38401 ttcagaaact ctttcacact caaagtgggt tctctgctat ccagagttaa aggattttta
    38461 ccctgaagtg cgttaagtgg attatagcga tacagtaacc aataacctgc atcaactgcg
    38521 gttttctgtc ttttttgtga ataagacata tcaataccat gattgatgca aggagcatag
    38581 gctatgatta tggaaggacc aggataggct tcggcttcca ggatagcgtt tattgcttgc
    38641 actttatttg ctcccatagc aatagaggca acatacacat agccataggt catcatcatc
    38701 atacccaggt cttttttatt ggtattcttg ccagcttctg caaagcgagc aatagctcct
    38761 aacggagtag attttgaggc ttgtccaccg gtattagaat agacttccgt atccagcacg
    38821 aggatattta cattccgatt tgatgccata acatggtcta aaccgccata accaatatca
    38881 taagcccaac catctcctcc aattgcccag atagagcggt ctataagatt atcctgaagt
    38941 tctatcactt tttgcaagag agctttacta tctccctcag cactattcaa agcttcagga
    39001 agtagtttct taataaggtc agcattttct ttcgcttgct tatctactgc atcccagtgt
    39061 tctaaagcat agttaatagc ttctttcagc tctggtttta gctctttttc taaaagtttc
    39121 tctaagtaga tttgcaataa cttacgatta gaatttactg ccagacgcat accaaatcca
    39181 tattctgcgt tatcttcaaa cagcgaattt gcccaggcag gtccttttcc tgattgattc
    39241 ttggtataag gaattgtagg gaaagttcca ccccagatgg aagaacagcc tgtagcattg
    39301 gcgataatca ttctatcacc aaaaagttga gtgagtaatt tcacataagg tgtttctcca
    39361 caacctgcac aggcaccaga gaattccagt aagggttgct tgaactgtga gcccttgacc
    39421 gtggtttctt taaaagagcc agtatagtca tagggaagtt tttcaaagaa ttcatagttt
    39481 tcttgttctc ccgcagctct ttcatcttca atgggagaca tctctaaagc tgccttaggg
    39541 cattcattga cacagacttt acagctctga caatcatcaa tataaacttg aatcttgtat
    39601 tgataacctt cagcaccaat agccttaagg gtattaaagg aggcaggggc atcttttaaa
    39661 tcatcctctt tgataagttt tgaacggata gcggcatgag gacatacaaa ggaacactga
    39721 ttacactgaa tacacttttc ggcaatccag tgaggaacta aaggtgcaac tcttcttttt
    39781 tccagtttgg cagttcctgt ttcaatgact ccatccaatg gcatatcgga aaccttaatc
    39841 tggtcacctt tttctctcat aacgggttct atcactctct ttacgaaatc agaagcgtca
    39901 tcaggaacaa gctttttatc aggtgcaaac ttctccggaa gactttcggg gatatcaatt
    39961 ttaaccagcg cttcattaac tctatctact gctagaagat tcatatttac tacatcttca
    40021 ccctttctgc tataggtctt acgaatagag tccttgatga gagcaatggc ttcatttctt
    40081 tccagaatac cagaaataag gaagaaagca gtttgcatca cagtgtttat tcttccacct
    40141 aatcccacat cttctgcaat ttgcaaagca tttatgttat agaattgaat cttgcgattg
    40201 atgatgactt cctgcatctt acgagtcaag ttttcaaaga cttcttctct attccagtga
    40261 gaatttagca agaacactcc attctctttg attccttcta aaaggtcaaa acgaccaata
    40321 aaggcaggat tatggcaggc aacaaaatct tcctgataaa caagatattg actctttatg
    40381 ggagatttac caaaacgaag atggcttcgt gtgataccac cactcttttt agaatcatac
    40441 tggaaataac cttgaacata aaggtctgtg tggtctccaa tgatttttat actgttttta
    40501 ttagcaccaa ccgtgccatc tgaacctaaa ccccagaatt tacagttaat agtgtccttt
    40561 ggactggggt caagaggttc atcaaaaggc aaacttttat gggtcaaatc atcttctata
    40621 cctacagtga aactatgaaa tcccttttcc attaagtgct tatagacagc caataccatc
    40681 ggaggagtaa attccttact ggataaacca taacgacctc caataatata aaggtcaggg
    40741 cgattcttta atgcagatac cacatctaaa taaagaggtt ctccaatgga ccctggttct
    40801 ttagtgcggt ccagaacagc tatccctttt acagtggaag ggagaactga caggaaatag
    40861 tcaatactga aaggacgata aagatgaact ttaatcagtc ccagcttcat atttcgttct
    40921 ttattcagaa aggtaatggt ttcttctatg gtttcacagc cactacccat tgcgatagta
    40981 atatattcag cttctggatg tccaacatat tcaaagggtt gataatagcg ccctatttta
    41041 tctcctacct gtttcattgt ttctgccact atgtcaggag cagctaaata gtgcccattc
    41101 acggtttctc taccctggaa atagacatcg ggattttctt gacctacttt tatccgtgga
    41161 tgatctggat taagtgcacg ttgacggaaa gcttctacca atttgggatc attcaattca
    41221 gctatcgtat cataatctat cacttctatc ttttgaagtt catgtgaagt gcggaatcca
    41281 tcaaagaaaa ctacaaaagg aatggaagtt ttcattgtgg caagttgtgc aaccaacccc
    41341 aggtccatca cttcttgaac actattacat gccaggaaag caaaaccagt attacgagcg
    41401 ctcatcacat cagaatggtc accgaaaatg gataaggatt gagcagccag agagcgagcg
    41461 gttacataaa atacggtggg aagcatttct ccagcaatct tatgcatatt tggaatcatc
    41521 agcatcaaac cctgagaagc tgtgaaagtg gttgttactg ctccagcaga gagagcaccg
    41581 tgaactgctc ctgcagctcc agcttcagat tgcatttcta ttacatctac tgtggttccg
    41641 ttgagattct tttttccact tgctgcccag gcatctacaa gctctccaat aggagaagaa
    41701 ggggtaatag gatagatagc agctacttca gcaaaagcat aagcgatatg tgccgcagca
    41761 gtattgccat ccattgtggc tttttttaca tcagccataa gataactcct tatatatcta
    41821 ttttactatt aaaatcaaag tttttttaac cttagttctt gaatggacta tactgtcaat
    41881 tgatttgaac taattaaaaa gctatctaat tgtaattctt agttctttga atctttcttt
    41941 ccattctccg gaagcattat cacacttagc ttattatcat tgaatctcaa atagctattt
    42001 gcagcagaga caatactttc tggggttaaa cggtctatat attggggtct attggtaaat
    42061 tctgttaatg gcttccccag ccagatatta gtccttaatt gattaatcca gtatctatta
    42121 gaccttatat tctgtttaaa ttgctgtttg agtgtttctc gcgcagaaat aatataacgc
    42181 tcatctataa aattgctttg taaactatct aaagtggcaa aaatagcatt attaagttca
    42241 tctactctca ctggtgcaca tgccataaca atattcaaaa gatagtaagg tttaggatag
    42301 ggagtcatat cagaccacgc ttggattaaa tagacaccac ttctttcttc tcttatattt
    42361 tctcttaact tttcattcat aacatatagc agaccatcca tcagcacatc attctcaatg
    42421 ttaggtttat atgaaccatt ggtgatatga gacacaaagg cacgatcagt cgcacctttt
    42481 gaaaatcgca cttctttttg tccacttata ggatagatat ttacatcttt aatctgctct
    42541 tttctttttt tagtgggtaa attccccaga taaatctggc agtattgttt taagagtttt
    42601 tcatcaaagt ttcctacaat tatgaaggaa aaatccgata aatcggcaaa gcggtcacgg
    42661 aaaatgcgtt ctaccgtgct catttcaatc ttatccagat caacttctgc aagattaacc
    42721 cgataggggt ggttattata aagttcctta tcaattttat ccataaagac atttaacgga
    42781 tctaagttcc ggttttcata ataggtctta gtacgctgga taaaagattg aaaatcctgg
    42841 gcattaaacc tgggattggt ggtatattga tatatcattt gaaacatcag ttccatatct
    42901 tttggtgagc aggaagcatc cagggtttcg gtgtagggac ccacatccag agaaacacgc
    42961 actactttat cagcggttgc tttagaaagt gatacggcat caaaagaacc aaatccactt
    43021 tcttttagat agctggaaac aagttctgct gcaggaatat cttctttatt atagcgagta
    43081 taacctccag gactgatggc agaaagcagt acttcatcat tcttaaaatc tgtctttttt
    43141 gcatagattt ttataccatt tttcaactgc cagtaataga tacctgtttc aggatcgtag
    43201 ctttcctttt tgattttcac aggtttgggt agatgagaca taaggggagc ggataaagct
    43261 ttatcctcat aggcttcaag ttcctgggtg cttatatgtt gagcaatttc cagtaatctt
    43321 tcctccgaag ggtaggttaa accttctttc tctggagcac caatggagat gcacatattc
    43381 ttttcctgaa tcagttgagt aactatagca ttgatttctg gtaaagttaa ctctgttaag
    43441 aaagatttcg taagttctaa atcttgctca gcacttaaag taacatcatt ataagataaa
    43501 tcaaaaacca gttcccaagc aatctgactg gattcacgag tttccttttg gtctacagcc
    43561 tgttgtagtt ctcttaatat ttgagattta gctctttcaa attcactgtc caaaaatcca
    43621 tatctttgta ccctttctgc ttcagtaagc attgtggtta atgctgtttc agctttgtta
    43681 ggcaaaaaca tagacataat tgctgcggta gacattgtcc ttagcatagg atattcataa
    43741 gccgcagcaa atgaataagg gggattagga ctcttgctaa attcatctaa acgggaattt
    43801 aacatcgtgt aaaacagact gttctttaaa taatttagat agctcccgta agtttggact
    43861 ggactgggtt cctttttcca cattacattc aatatattat agggatattc tgggtcagtt
    43921 gccactactg ccacaggtgt taggttttca ggaactttga actcctgttt aggtcgtgga
    43981 tttaccggtt tcggaatgac accaaaatac tgagttacca gagctaaaat ttcctgcggg
    44041 tcaaaatctc ccactataac aagtttttgt aaatcgggac gataccaatc tttataaaat
    44101 cttaatagag tgtctctcgt gaaactacta ataacttcaa aagtgccaat tggtgagcgt
    44161 tctgcatagc gtgaaccagc aagaataact gaattttgtt tatccctgat tctactttca
    44221 gcatcttgtc ccatacgcca ttcttcaatt attactcctc gttccttttc aatttctgcc
    44281 gggtcaaaag tagcctgata tgccatatct gatagtatca tcaatccctt attcaatttc
    44341 tctttatcat cagtggggat tttaaatgta tatattgtat aatcataact ggtcatagca
    44401 tttagaccat tagcgtatcc cattccgatg gaagaaagat aatttaccag ttctgattta
    44461 gcaaaatttt tcgtgccatt aaaaaccata tgttcagtaa aatgagctaa accgcgttgg
    44521 tcatcatctt catctactga acctacatca acataaagac gaagctctgc cagatgagaa
    44581 ggattgggat tatgctgtat gtaataagta aaaccgtttg gcaactgtcc tgaaagaatt
    44641 gcaggatcct gttgtagagg ttgttctaaa agttcattag gtagtgctgg gaggatggta
    44701 aaaagtgcac aaaatagcac acttatgaag attaatttct tcaagttcat ttagaatagt
    44761 attcctttat catttattgg ggattttcta cggtaaccaa aaattgctgg tccttctgaa
    44821 gtattgcatt ctcaccacca tattgataac gcgttccatc ttcaagttct atcagaacaa
    44881 agtagtagaa attattatta ggagttacat aatctacatt tttgaaatta aaactatgtg
    44941 gtgcgatagg gtcaatagta tagtgattta caacaccaat aaagttgtgc ttataaatat
    45001 ctatttttgc tatccataag tcactattat ttactacttt aatgcttgca cgattgggtt
    45061 gaatgtaagc attgatagtc ttattaacct tgatggttac agtcgttgaa tcagtataag
    45121 attgtgtata atcatcatat atctgatagg tctcacctat catccagaca ggaaccttct
    45181 tttctacatc cgggttaaaa atactttcct tcttggtttc tatctcaaag gtataattag
    45241 aattacctgg aatggtcact tcatcttgtt catccacttt aacataaaga ggataggagg
    45301 tttgatttat taccctaaaa gcacctccgg aatcacaacc cgtaataagc cataaagcca
    45361 ttaggggtaa aatccataat gttaactttt tcatggtaaa cctctcattc ttattgcatt
    45421 gtattatgca taaaaacact atcttttata tattgtttga tgtcaagctc taaattcttt
    45481 taaaaaatat taagaataat taaagaattt ttattattca attctttcta caaataatat
    45541 tgttctatat ttattgttta taatacacct tattaatctg agcctttact ttgatttatt
    45601 tattcagcta aaatatatag aattgatagg aatttatatt atgctatata ttttatatta
    45661 ctaaatcaca ttattgtcct ttcattcaaa tctattaata tctctggttg aaattaataa
    45721 acatcctacc tctcccttag ctctttatta cgtaaagtct taagagagag acagatagga
    45781 gacaagaaac aatttatctg gataatgata tgattatcag agagttatag agtataattc
    45841 aatatagtta aagattattt atatttagta taataattgt ttttattaat tataatttag
    45901 gtattaagtg atttataaat gacaaaagca aatccataga tttatttttt tcaaagaggg
    45961 aagagattaa atttaaaaat cttctcttga cagttggatt actttaagtt tatctgttat
    46021 gatgaaagtt aaaaggtaat ttattaataa tcaggaaaaa aggagtgaga gtgagtgtat
    46081 ataatttatt taaatctgat gaagaaatgt ttttaggttg cacaaaaagt gtaaaaaaac
    46141 aatattgtat cctactcttt aatataaaat tacaataatt attttcgaat atcataaaga
    46201 taaagacgat atgaaaacat cacataagat aatcattggt gattccagaa ggatgaagga
    46261 attaaatgat gaatcagttc atcttattgt aacttcacct cctta
//
LOCUS       DAORZF010000009        40365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_790_length_40365_cov_335.917489, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000009 DAORZF010000000
VERSION     DAORZF010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 40365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40365
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_790_length_40365_cov_335.917489"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(235..1884)
                     /gene="hcp"
                     /locus_tag="PK015_02190"
                     /gene_synonym="priS"
     CDS             complement(235..1884)
                     /gene="hcp"
                     /locus_tag="PK015_02190"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOL33318.1"
                     /translation="MSMFCYQCQETSRNQGCTVRGVCGKSPELCHLMDLSIYAMKGLT
                     FVSSKLLEHNIYHREDALIIMQGLFRTITNTNWDEEQIKLSIANIIKLRDKRKAELEE
                     VAKDRNIKYPDAALFHLSLEEYESFGTQVGVLSSENEDIRSLRETIIYGLKGISAYGD
                     HSAMLGYYDDDLNQFIYKALAATLDDNLSPEQLTNLVIKTGEYAVKVMELLDRANTET
                     YGVPEPTTVNLGVGNRPGILVSGHDLKDLAELLEQSEGTGIDIYTHGEMLPANYYPAF
                     KKYSHLYGNYGSSWWHQLDEFEKFNGPILMTTNCLVPLRKDNTYLNRLYTTGMTAYPG
                     ATHIPDREPGKAKDFSAIIAQAKKCLPPTEIEKGTIVGGFNYRTVLSMADKIIEAVKS
                     GAIKRFVVMAGCDGRMPSRQYYTEVAKKLPSDAVILTAGCAKYRYIKLNLGEIDGIPR
                     VLDAGQCNDSYSLAVIALKLKEAFGLEDINQLPIAFDIAWYEQKAVAVLLALLSLGFK
                     NIRLGPTLPAFLSPNVAKVIVDTFGLQPISNPDDDVKAIMN"
     gene            complement(1906..2223)
                     /locus_tag="PK015_02195"
     CDS             complement(1906..2223)
                     /locus_tag="PK015_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOL33319.1"
                     /translation="MESRFWKDLPLVRDKDGVRGTKIYSQPEGEIVHMYLEAGAKLPL
                     HNTPVNVAFYILEGNPTIIIGDEEQSFAPDTIIDSPKNVPHGIINNSDKPARLLVIKM
                     PKP"
     gene            complement(2235..2819)
                     /locus_tag="PK015_02200"
     CDS             complement(2235..2819)
                     /locus_tag="PK015_02200"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOL33320.1"
                     /translation="MTERQEQIILTAIALIAREGYKNLTIKKLASELHLSEAALYRHF
                     VNKEDLLLSIMHYFEDISRNVLQELQKTKLNPLEKVHFFIMNRYELFTSNPDLAQVMF
                     SDEMFYYDPVFFKQFQKISMNHREVLLNFIEEAQKEGFIQSGINSEQIFTILIGSMRF
                     LITQWNLSGRKFDLGKEGDSLFQTIKNLIAEKKI"
     gene            complement(2944..4599)
                     /locus_tag="PK015_02205"
     CDS             complement(2944..4599)
                     /locus_tag="PK015_02205"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOL33321.1"
                     /translation="MKRNIFILFLLVVCVALNAGDYFIGSGTTNQNKVPVYGYNNYGW
                     SKFFYTSTETTGAGMSGTVQITGIAFKVSNSISNYTMDNQQIYLAHFYSTSYTSATAG
                     YPNNSYYTNVYNGPVTFNGPGWVHIQFTTPFNYDSSYGFEILWENHDGSKIGGPPSFY
                     YTALSNSSVYIHQDASFPTGSGQRSNYHPNIWIETPVTDIPPAAACVFPPNESDGHNV
                     NITLRWNNMGGRPDHYRLWLGSNNPPSNLVNGEIVYEEYYTPSTYLDYGTTYYWRVVP
                     HNDFGYAIDCPIWSFTTMADPTIYTFPWLETFDGTFKPTGWTDHSGGLVDPIVLGANG
                     SSQWQQDDWLNITSTDKAAKINIWGPISGWLISPLLYISDDQVLSFDAALLKYNQPPT
                     GTPHDTTGIDDRFAVLIGDGFSWSTANILREWNNDSSPFVLNDIPESGTHISIPLAGY
                     SGRKRIAFFAGSTVSNADNDFMINNVEVKSLLQTPVVTITLDNATNQLILSWEAVPNA
                     TSYKIYKSSQPYGDYNYIDTVYSTQYQTIATEAKAFFKIIATN"
     gene            complement(4620..7886)
                     /locus_tag="PK015_02210"
     CDS             complement(4620..7886)
                     /locus_tag="PK015_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOL33322.1"
                     /translation="MYKHLALIIALLSIFTICLGQWHIDENFDNITTLPAGWTIHDDG
                     DGMVWRNLNNATHAHSGTRAAFCDNYLPNQNADWLITPQLNIAEGDSLIFYTRSWIST
                     EQLKVYVSTTGTAINNFTTQLVHLQNIGTTYQRVSLNLSNWANQIIYIGFLWNCINYG
                     ILIDDVQIGQPLIIQPALNLPESFSFIQGETLTVDFTPFITCTDINSCTLSVSGNVNI
                     NVQINGRIVTFSSPDFTGSENITFTMTDNISGLSASDTVVVEVLPVPTIDLGIIEIPS
                     PREFEYQNHSFIPALKVQNLGTASYSGMLSISCNILNSSGTSIYSNEEFHLLDLQPGN
                     SVTITLSTSCVITSEGNYNAIFSLISEDGNPANNTMEESFTVVNRITQGGPDTFGYRF
                     IDSNDEAGPEFNWIDISSTGTSTIMYQVPSWEGDDNFSEAIPMGFPFPFYGQLYDNMY
                     VDINGEILLSESNWQKPYPNTGWDSDGNMFNYAYPIPGYNAMPTLIAVYWDDLFAEQG
                     ISDIYFQTFGEAPNRYTVVQWNNLRFLAGTGGTPQLKFEVIFYENGDILMQYHTTHTG
                     QSGSNIPHQNGQSATVAIQNQDATIGLCYLREIVINNQYQGVEPPGNLLHDNLAIKFF
                     TGIDEQPPHLTYTEAGNTFNRTPLLKVNAIDLSPITSLVLHYNTGEGWQSMEYTSQSG
                     TLYYFQLPELPQGAELNYYFTATDSEGNTGSLPEDAPTHFYSFKILPTADTEVLIAYS
                     GRQDYQRIELPVYTNLLDNLNISYDIYNWEEYDSFRFPLQYKAILCYANVGSHTALSD
                     TLSLALMQYMDSGTTTEPKNVFFASDGFAGSTHANPNTSLIKKLFNAYFRSYYVPTGS
                     GGGTNGLAGPEVFSYQYGSILCYTYSPIGIVGNEYQVYADSPDCIFRYDSCPDWYASE
                     VQYPEIGAQSAFLFEDGPIDGHAYLYHGVCATTVTLPIYKAFYFSFDFSQVYQEAQRY
                     ELMSDLMQWFGISPDANEDYNIPQVETKINGNYPNPFNPETTISFTLAKNAKVKLDIY
                     NVRGQKIKTLLNDNLSAGIHNCIWDGKDDNGQIVSSAMYIVRLDDGNKIKQHKMMLIK
                     "
     gene            complement(7900..9267)
                     /locus_tag="PK015_02215"
     CDS             complement(7900..9267)
                     /locus_tag="PK015_02215"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOL33323.1"
                     /translation="MIKDIAVLNQHSQIRELGSELRYNILRELIRGAATCQQLADIYG
                     ISKQKVHYNLNKLLDEKLIEIVPSNQVRAKEVYYRATAKNYILDFAIGQNINDHSLSK
                     RDIIDNILTQEYHINLNSIAAKLLEDSLKMKPQESLLIVSGSYNQPLVEKILVEAGRR
                     SINTTWIYQSTEILKAKYEEYSLSAFDADYKQFNRLLAKHNVYLNLNGEARYIELNDP
                     QKQRLRSKHFAQSRKIIQQNKIKIAIMPGLLPNTLEENTIESEIQFWKALDIDYDKLY
                     QRTHNLCLEMSKHNAIQVSNSDTGFSFTIQKILAECGSFGDSEFQSPTINLPGGEILL
                     IPEPDSLNGEITGDLAYISGKVVERPWIRFENNKIAEFKAESNEALLQEAIITGGKDG
                     ATVALVCLGTNENVHLTNIDSSYKHKCKGLLTVYWGENISLGGNVAGENEWYIQIENP
                     QLSYL"
     gene            9479..10543
                     /gene="hydE"
                     /locus_tag="PK015_02220"
     CDS             9479..10543
                     /gene="hydE"
                     /locus_tag="PK015_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HOL33324.1"
                     /translation="MRPNRQEVIEMLSLKKKDEIEALYKRAYEVKKQYVGTKVYLRGL
                     IELSNICTRNCYYCGIRKDNHLLERYYIDKEEVLKEARWCFEQHYGSLTIQAGERSDK
                     SWIDYIEELIKEIKTLTDGKMGITLSLGEQSEETYRRWFEAGAHRYLLRIETSNPELY
                     KNLHPAENSFENRLECIRSLKKIGYQVGSGVMIGLPNQTVEDLADDILFFYDEDIDMI
                     GMGPFIPHHQTPLAYLLATFHPEEALEQALKMIAATRIALKDVNIASTTALQALHPKG
                     RELGLLAGANVLMPNITDTRFREGYQLYEGKPGLNENALAIRKALEESIFSIGESIGY
                     DEWGDSPHFKSKKNTPSENP"
     gene            10622..10933
                     /locus_tag="PK015_02225"
     CDS             10622..10933
                     /locus_tag="PK015_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002107277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOL33325.1"
                     /translation="MQNMNQIMKQMQNMQKEMMKSQEELEGKIFEVTTGGGMIKLEMN
                     GKYEVKSIKINPEAVDPDDVEMLEDLILSALQEAHKQVSEALDEIMGKLTGGMKIPGI
                     F"
     gene            10934..11530
                     /gene="recR"
                     /locus_tag="PK015_02230"
     CDS             10934..11530
                     /gene="recR"
                     /locus_tag="PK015_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOL33326.1"
                     /translation="MIISPALEKLIQALTRFPGIGKKTAQRMAWYLVSQDKSFGLELA
                     EIIKQTVENITTCSLCNMLSESDPCPFCTSSERTDSLLCVVESSADIQIIENMNEYKG
                     RYFVLGHLLSPIDGYGPEQIHSAQFLQAIERFHPQEVILALKPSPEGEATIHYLWELL
                     KDKDIKVTRLSTGLPFGGDLEYSSMLTLSNAWKRRYPV"
     gene            11502..12140
                     /locus_tag="PK015_02235"
     CDS             11502..12140
                     /locus_tag="PK015_02235"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOL33327.1"
                     /translation="MPGKGVIRFNMFTGIVETTSRVMRTLNSSGLKAITIELPKSFND
                     LKKGSSIACDGICLTVTQLNTDSFNVEIMQETVQKTTAGNWNKGTILNLERALKLEDH
                     LDGHLVLGHIDRTTTLLEKKTVSATTYLRFSLDSRDRELVVKQGSVALNGVSLTVAEL
                     NSSSFTVALISYTLDTTNLRYLSPGANVNLEYDIIGKYIQNLYHSSGFKNKL"
     gene            12137..13420
                     /locus_tag="PK015_02240"
     CDS             12137..13420
                     /locus_tag="PK015_02240"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOL33328.1"
                     /translation="MKKIFKLSIILCLVIFLASCGSKKSPTGGPEDLSKPVVISSLPP
                     QFGNISEGRIEINFSKPLDKTSIPHSVYIYPPVMNKKITVDKNSVIIQINENLLPNTN
                     YYVIISTRLKDIRGNSPDENQTLVFASGKLNQLRLSGTIDYEHPGDNSLPVQMSLLSE
                     DSLLVLSQVARGPSYAIEPLNPAHYILRAYIDKNLNGRYDFTQEPYFEQPVRVEHPTN
                     LNLFLAYADTTKPNIKLVTAQHNREIEITFTEAIKGYRSIKINRLDNKEELPVLLSYL
                     EEDKLTLLTASQDAFAYTLELYGVEDKKGNVTPVIKSEFRSSTIMDTQPPQVVYSSPR
                     NGTSVKSLEPILEVCFSEVIPRASVKASLRASETKTEIPLEVLQSDSRIYRFKPQKPL
                     QNYRSYVLTISATDISGNSMPESFNLNFLPLIYQD"
     gene            complement(13440..14867)
                     /gene="gltX"
                     /locus_tag="PK015_02245"
     CDS             complement(13440..14867)
                     /gene="gltX"
                     /locus_tag="PK015_02245"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOL33329.1"
                     /translation="MVRVRFAPSPTGYLHIGGLRTALYNYLFARHTGGTFILRIEDTD
                     RTRIVPDAMENLIHTLQKLDITFDEGPIIGGNYGPYIQSERLDLYKKEAQKLLDAGFA
                     YRCFCTPETLAQMRAEQQAKGEFVKYDRRCLNLTPAEIEAKLAKGEPYVLRLKMPEIR
                     TFRFNDIIRGEVEMDSAQSDDQVLIKSDGYPTYHLASVVDDHYMDISYVIRGEEWLSS
                     MPKHLWLYECFGWTPPQWVHLPLILNPDRTKLSKRMNDVAVEDYLERGYLKEAIINFV
                     ALLGWHSADNREIFSLEELCQEFTLERVNKSGAIFDLNKLNWMNGWYLRNLPLDTIVE
                     RSKPYFEKEHLAIPDSDKLTKIVATARERCTLLSDLVQYSKMFLCPQPLSTESLDFLK
                     KEDSRKVLSWFQSHLPSGGDIDAEQLNQLVQQGIKDLGFKGKAFYKPLYLALLHQDHG
                     PELPAIFSILGLKEVHHRIQEVLEL"
     gene            complement(14889..15497)
                     /locus_tag="PK015_02250"
     CDS             complement(14889..15497)
                     /locus_tag="PK015_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33330.1"
                     /translation="MMIVKPDFTLDEISDIILQEIKLHPRCHYLDVFKLLYQAFYGPS
                     HIEPQRDTIIYNLKRELNDMKWKTDVPYQDIGVGKGFIRLNLISIISLPDEDLKQNSM
                     LLNKKIPTEYLKDSEDKIKILADCILQSRFENEINWNDWIETWKNCIPVVKSIIPATP
                     EEGLMIEDCLTKKIMPTHSEEYRQWYDPHYRVIHHSLLRFIF"
     gene            complement(15484..16953)
                     /locus_tag="PK015_02255"
     CDS             complement(15484..16953)
                     /locus_tag="PK015_02255"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOL33331.1"
                     /translation="MIYLYNAHIYHNAKFDAQLSGLLIDKDRIVKLCFNNDKVNPDWE
                     KINLNGAYVYPGFIDAHTHCFSGGLYLSGIDLSECTSIKEVLEHISNSLKNNQQYIFA
                     WRFDEEKIAEKRFPTRQELNSVCPDCPLLLRRIDGHSCVLNSKARAIIPGLTSSNEIL
                     IAEDNDRAVNWLQDNLSEEEIIEAYKNAAQAALKGGFTTVHTMIGDAQMSNQHYKLVR
                     DHLNEFPVSFELYPQSFNIRDALELGAKRIGGCILADGSIGSHTAAMTLPYKDKNTRG
                     ILYHNDEFWQEFVAQAHKEHLQVAVHCIGDAAIHQINSAYATQDREEVKELRDELIHC
                     EVTPDSLIQEIAKSGAVAVMQPAFDLLWGGNSGLYSERLGERYKIMNRFQSLSRQGIS
                     ICSSSDWYVTDMNIAMSLYALIHHHNPQEKLEPIEAIKSYTENNSWLNHTEESLGIIE
                     EGYIADLSVMDTDFTKLFDWNKVRTLLIIKDGNIVYDDR"
     gene            complement(16958..17563)
                     /locus_tag="PK015_02260"
     CDS             complement(16958..17563)
                     /locus_tag="PK015_02260"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOL33332.1"
                     /translation="MIHNLLNDKKVVLASSSPRRLELFKMIGLQPLVIPAKIEETING
                     EKPYIQVMHNAKNKASTIAALIDSETVVVGADTIVVLENKILGKPENVMQAKEFLTLL
                     SGKTHKVYTGVCICWKTLQAINYERSLVEFSPLTETDIDAYIATGEPMDKAGAYGIQG
                     FGSQFIKRITGCYYNVMGFPINLFYRMLSSIFESNKIDNPG"
     gene            17707..18792
                     /gene="serC"
                     /locus_tag="PK015_02265"
     CDS             17707..18792
                     /gene="serC"
                     /locus_tag="PK015_02265"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOL33333.1"
                     /translation="MERVYNFNAGPAVLPEEVLLEAQADLFNYKGMGLSVMEMSHRSK
                     EFQAIIDDAYARVKRIYGLGDDYEVLFLQGGASLQFLMVPLNFAVEGKEANYINTGQW
                     SKKAIAEAKKIGKAVHIAASSEDRNFAYIPRSWQLSDNPSYLHITTNNTIFGTEWKFD
                     PEIPEDIPLIADMSSNFMSKPIDIQKYSLVYAGAQKNVGPAGTVVVIVKKSLLEKAIP
                     GLPTMLDYNIHAKNGSLYNTPACFIIYMVGLVLKWIENFGGLEKIAENNKLKAGYIYD
                     TIDESNGFYRGTVDKEDRSLMNITFRLPSEELEELFISEAKKNGMIGLKGHRDVGGCR
                     ASCYNSLPLKAAKTLSEFMKEFQRQHG"
     gene            18874..19497
                     /locus_tag="PK015_02270"
     CDS             18874..19497
                     /locus_tag="PK015_02270"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOL33334.1"
                     /translation="MSLLDFIMRGGFLMYVLVAISVVIIAIVIEKYYQIHKVRKANEK
                     LSFYLAQQDKPENIRAILRIHGQSSPLGLMLDKLYNADTDDIKLIQESMEAVGNRELH
                     NLEKGMDWLSTLSTIAPLVGFLGTVIGMVQVFMNIQGQGQNTVDISTLAGGIWVALLT
                     TVGGLVVGIPSIIFYNNLVQKLDDLAKDMQHEVMEHEIRYRKMLEKA"
     gene            19502..19903
                     /locus_tag="PK015_02275"
     CDS             19502..19903
                     /locus_tag="PK015_02275"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOL33335.1"
                     /translation="MRLEISKKKISSTTLVSMTDMMFLLLIFMVLASNFTAQTGLPLK
                     LPASVSQERQVHQVLQIQYLPNKTIVFQGTEYSLENLGPALQKEFRSKDQVVRLSADR
                     STPLQDVITIMDIIRGVGFERIFVGTEPIKE"
     gene            19908..20621
                     /locus_tag="PK015_02280"
     CDS             19908..20621
                     /locus_tag="PK015_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33336.1"
                     /translation="MTLPSGKYSPWLISIILHGILLLIFSFYYIKPQVAIHWYQIEPY
                     DPLTQWEETPVIKDQARESIKEEVSPLPQISNKVIAPQKSEVPPQTLEQETIPQPLAG
                     EVMEAPELSTQPVPKTTAPVVNNPLAYGFLKGLTGGDKSSAAGTVNVSFGKGKGRVRF
                     DFPQDYRHSLGAPGTVTVRFKVDQYGKPIMSTVDAIEQSGPRYFAEARKILEMYRDKF
                     HIIGEPQPGIECELTFIFQ"
     gene            20621..>21004
                     /locus_tag="PK015_02285"
     CDS             20621..>21004
                     /locus_tag="PK015_02285"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOL33337.1"
                     /translation="MIEFKIPALMGILNITEDSFSDGGKFINLKDALLQAEKLISEGA
                     DILDIGAESTRPGAEPVPEDIELQRIIPVLTEIRNRWQDIPISIDTRKSKVAEAAIDL
                     GARIINDVSALRFDPEMADMLSSHPQ"
     assembly_gap    21005..21104
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <21105..21302
                     /locus_tag="PK015_02290"
     CDS             <21105..21302
                     /locus_tag="PK015_02290"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOL33338.1"
                     /translation="IGVSRKRFIASLDNSSVEQRLGGTLAAGLVAVLKGVEILRVHNV
                     QAHRQFFQVLKEINEVSEWTS"
     gene            21290..22036
                     /gene="cdaA"
                     /locus_tag="PK015_02295"
     CDS             21290..22036
                     /gene="cdaA"
                     /locus_tag="PK015_02295"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOL33339.1"
                     /translation="MDILIPKFADIVDIFIIAFLIYQILLVLRRSGGWQILVAVVAII
                     ILATIATALNLRMVSSLLNSISRYWALALIILFQPEIRTLLSKINLSRELNLSLRKRE
                     TNYVYMPLIDAISSLSFRKIGALIVIENRRKLTEYINNGEIIDAALSLRLVLTIFNPK
                     SPLHDGAIIIRGDRIIAAKVVLPLSKNPEYIKHFGTRHLAGIGITEVSDAIAIIVSEQ
                     NSQISVAQNGKIKVNVAYEELLQILMDATS"
     gene            22057..22677
                     /gene="coaE"
                     /locus_tag="PK015_02300"
     CDS             22057..22677
                     /gene="coaE"
                     /locus_tag="PK015_02300"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOL33340.1"
                     /translation="MSQPILIGITGNIGSGKTTFCNLLEEKGFKVIYADEIAQQQLNQ
                     PDTLKTLIKRWGKGIVKNGQPQRDKIAEIVFNNKSELDFLNSIVHPKTLSALQQIVDN
                     STEKYLFFEIPLLFEAGLELCFDYIILIRAPREVRINRLLQKGKETREQIEARINAQI
                     DDQDKIPLCDLVIDNSKDLIELNNQLNSLLSTLKDIKPKEKIPFSL"
     gene            22882..24090
                     /locus_tag="PK015_02305"
     CDS             22882..24090
                     /locus_tag="PK015_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOL33341.1"
                     /translation="MKKFFQRIVDFLGMNSSLAGMLLMVILVGMGEKMAERFLPLYII
                     AMGGTPIIIGLLNGLDNLLSALYSYPGGWLAEKLGYKKALAIFNLMAIFGYLIVVLFP
                     TWQAVLIGAIFFISWTAISLPATMSLISSVVPANRRSMGVSLNSIVKRIPMALGPIMG
                     GALIMKFGRIQGVRYAFIVAIILAIVALFVQQKLIVEPPVEKKTKQKKGSRRFSKNLT
                     NLLVSDILVRFCEQIPYAFVVVWVVENNHISEMQFGILTAIEMVTAMLIYIPVAYLAD
                     RSTRKPYVAITFFNFTIFPIVLLFSKTFPALIFAFIIRGLKEFGEPTRKALIMDLAPE
                     DSKAWTFGTYYFIRDVIVSVAAFAGAFLWKVSPALNFLIAAGFGLLGTLYFILKVHST
                     PVPQTAINPE"
     gene            24654..25721
                     /gene="dinB"
                     /locus_tag="PK015_02310"
     CDS             24654..25721
                     /gene="dinB"
                     /locus_tag="PK015_02310"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOL33342.1"
                     /translation="MKKIIHIDMDAYFASIEIRENPSLKGKCVIVGGLPDSRGVVATC
                     SYEARKYGIHSGMSSYQAWKLCPQAVFVHGHFKLYKEVSEQIRAIFHQYTDLVEPMSL
                     DEAYLDVTENKINEPDAVKIARLIKQDIWNTTQLTCSAGVSYNKFLAKIASELQKPDG
                     LSVITPENTREILFSLPIEKFYGIGKVTAARMKKMGIKNGEDLYNQDLSFLISNFGKA
                     GVYYYQVVRGIDDREVITEFEPKTVSCENTFEKDLDDINDILAELEKLIERLINRLAF
                     QGIMGKNIFIKIKYDNFEVITRSCPLPEPTADRAILYEYAEKLLLSNWDHSRKIRLLG
                     VGVGKLDLERPEKEEQLEIPI"
     gene            complement(25729..26646)
                     /locus_tag="PK015_02315"
     CDS             complement(25729..26646)
                     /locus_tag="PK015_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOL33343.1"
                     /translation="MNKTFQKAYYYAFAAILAWSTVSTAFKLSLKHLTPLGLLLISSL
                     CATIFLAIIFLVNHKRDKTNKPSFNWKIRILSGLLNPFIYYIMLFEAYHRLRAQEAQV
                     LNYTWAIVLSFLSALFLKQKLRIKDIIALFISFFGVLLVSTQGRFWEFQFDDPVGSLL
                     AVSTSLVWAFYWILNMKDSLPAIKKLLGNFLIGTLATFFYALILGKLNYNGLFSSEAN
                     ITFGILGGIYVGIFEMGLTFLLWQKALEYSTSTASTANLIFLTPFLSLIFIFFILKEH
                     ISPATLIGLVLIVLSNWWQKSSRPAKVLK"
     gene            complement(26701..27786)
                     /locus_tag="PK015_02320"
     CDS             complement(26701..27786)
                     /locus_tag="PK015_02320"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOL33344.1"
                     /translation="MKRIFLLTLAVLLFLTGCATTRHRLSPQANVDLKTANVYYSQQN
                     VEEALKYYNRVLQDNPDHALALRRVADIYLYYGEQNPDKSLEYNKMAYEYYDRAIKIM
                     EKYEKPTEDELAAIRDMKKRKLSAWTRVYNKGIEELNAGNTQQAIKIFEQAAEMDPTE
                     LKPLNMLVNIYQNELKDMEKAGEYLFKLYEQNPENVEVIMNLGAYYYNKQDFEKAIPL
                     FEKVKEKEPTNIKNLLVLTDCYYASGQYDKAKNNIQLALIIEPRNTDALATAYDIAVK
                     MDDDEAALNYLKQLLDIKEDNIYYENIVILLNSMERWEELANYAQKWHKFDETNKMPV
                     QFVILAAQKMKNKALETEYTNILKQMK"
     gene            28036..28974
                     /locus_tag="PK015_02325"
     CDS             28036..28974
                     /locus_tag="PK015_02325"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOL33345.1"
                     /translation="MESTPQFVGRSLYDLDDYSSDEIMYILEAAKGMKEINLREYKKI
                     PTLRGKTVCTLFVEDSTRTRMSFELAASRLSADVVSFQATVSSLKKGESLQDTVNTLN
                     AMGIDLYCIRHSSPGSPQLVHKYSGKPVINGGDGRHAHPTQALLDIFSIWERLGNLKG
                     LKITIVGDILNSRVVRSDLIGMTKLGMEVTVCGPRTLMPANMEQIYGCKVEYDLAEAL
                     KDADIVMGLRMQLERMTEGLFPSLEEYSRHYVLSKETIKYAKKDALIMHPGPMNRGIE
                     ILPEIADSAQSIIVEQVANGVAVRMALMFLILGGKL"
     gene            28986..30260
                     /locus_tag="PK015_02330"
     CDS             28986..30260
                     /locus_tag="PK015_02330"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOL33346.1"
                     /translation="MTTLIKNGIAYINNRFSRKDILLKNGKIQRIAQTINLDKVDQII
                     DTSGKYVFPGFVDIHTHLRDPGQTYKEDIASGTRAAAHGGFTTLCAMPNTEPVVDNIA
                     SVDYVQRRAKDLGSARVFVIGAITKKSEGLEISEMATMKSGGIVAVSDDGNCVQNAKL
                     MLNCMRYATNFGLPLIIHAEDYNLSGKGQINGGKVATKLGLTGISPLAEEVIVARDIM
                     LAENCGAQLHIAHISTARSIELIREAKERGVKVTCEVTPHHLLLTEEACLNFDTNTKM
                     KPPLRTEKDRQACVKALKEGIIDCIATDHAPHADFEKAREFDQAPFGIIGLETAFPVL
                     YHYLVKPGYLSLERLVEALTSAPVKILNLPESALKTGNPADLTIIDLGAETIFTPESI
                     LSKSKNTPWLNQTVSSKVLYTFLEGRQTWKAE"
     gene            30260..30997
                     /locus_tag="PK015_02335"
     CDS             30260..30997
                     /locus_tag="PK015_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOL33347.1"
                     /translation="MQYKILPLEKIEKIRTNYIQITIKDEDLASQCNPGNFFQIKADI
                     TSQSWRLFKPISVYDIEEESISFLIKVIGGGTRALAALKTGDKLKIYGPLGNAFPLRQ
                     DSKILMVSGGTGYPPLAFLKKRLDSSNNILFLHGGASKDDVFPCDCSYTIDGSFGNKG
                     IVTKDIADLLKKESFDCIYSCGPVNMLKTLAQIVAPIPHYVSMEAYMACGVGVCYGCV
                     IPTNEGYLRVCYDGPIFEAEKILWKEL"
     gene            30994..31896
                     /locus_tag="PK015_02340"
     CDS             30994..31896
                     /locus_tag="PK015_02340"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOL33348.1"
                     /translation="MSELSTSLGKINLQSPILNASGTFGTEYFNFFQQGILGAYVTKT
                     ITLKPKIGNPPPRLYETEAGLLNAIGLQNPGLKSFLNNDLFDLREYLKIPLIVSFSGA
                     TQEEFIRMLEGLELHCGIYGYEINISCPNVENEGIAFGTNPDIVFNLVEKLARLTDRE
                     LIVKLSPNVTDIALIAQAAEAGGATSISLINTLYGMAINWETGKSRLSIPVCGYSGIG
                     IKPVALALTYQTARAVKIPVIAMGGIHSWQDALEFFYAGASAIAIGTANFSNPLTIPE
                     VYQGLLNFLTQRNLNLKDIIGKVN"
     gene            32030..32377
                     /gene="ssrA"
                     /locus_tag="PK015_02345"
     tmRNA           32030..32377
                     /gene="ssrA"
                     /locus_tag="PK015_02345"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(32832..32907)
                     /locus_tag="PK015_02350"
     tRNA            complement(32832..32907)
                     /locus_tag="PK015_02350"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32872..32874),aa:Gly,seq:ccc)
     gene            complement(32993..33499)
                     /locus_tag="PK015_02355"
     CDS             complement(32993..33499)
                     /locus_tag="PK015_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33349.1"
                     /translation="MPKNCRYIALFLLTIVFLNLFLPIVAENVVVRPTRVQLSLAQTI
                     DAETANQGDPVEFVVVHPVYQDGKIIVPAGARAYGRIEQVTQNGLLGKPASLAISLTT
                     VDAIDGSKLPIIASKTLKGESRTAISIGVSLFLCILGVFIKGENVTMQAGYIIDADIL
                     GGNVVNVP"
     gene            complement(33509..34318)
                     /locus_tag="PK015_02360"
     CDS             complement(33509..34318)
                     /locus_tag="PK015_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOL33350.1"
                     /translation="MNLRVFAILFILLFFISLNGEVTNNFAYADFLKSEKLYDLAIGE
                     YYRSMYNGSKEDTTRALFGIAECYAKSHKFIEAAKTYEKLEDQEINWDAIKLHTFLLT
                     DIKYYYESNKLIDKFIQALPKEKQDSLLIYKAVNLINLGKYNEAEETLNQISTDYPKS
                     RALDIQAIMDESLPLKFKKSGKAVFFQAILPGAGYLYCKMPQTALATLVVNSLFGYTI
                     MDNFKRGNTGSGVFFSVIGTGFYLGSIYGSLQAVSKYNKREMENFSNKIRP"
     gene            complement(34315..34683)
                     /gene="yidD"
                     /locus_tag="PK015_02365"
     CDS             complement(34315..34683)
                     /gene="yidD"
                     /locus_tag="PK015_02365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOL33351.1"
                     /translation="MRKSIGVILFFILITLSAISAKNNITNISIPSTLEKNKGIKLDN
                     PTTIIEYMVEFYQKAISPVKQQNCPMYPSCSNYGLEALEKYGLRGIFYTVDRLNRCGH
                     DLQYYIKVIVDDEIYFLDIP"
     gene            complement(34848..>35642)
                     /gene="guaA"
                     /locus_tag="PK015_02370"
     CDS             complement(34848..>35642)
                     /gene="guaA"
                     /locus_tag="PK015_02370"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOL33352.1"
                     /translation="DNGCMRYKEAERVQEAFSAIENFKINFVDASELFLSRLAGVSDP
                     EMKRKIIGSTFIEVFEKEAAKYENVGFLAQGTLYPDVIESVSFRGPSAKIKSHHNVGG
                     LPEKMGLKLIEPLRELFKDEVRAVGKELGLPESLINRHPFPGPGLAVRIIGAVDKEKV
                     QKLQLIDEIFIQELHNWNLYNQTWQAFAILLPIQSVGVMGDERTYENVCALRAVNSQD
                     GMTAEATEFPWNFLKYVANRIINEVKGINRVVYDLSSKPPATIEWE"
     assembly_gap    35643..35742
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<35743..36233)
                     /gene="guaA"
                     /locus_tag="PK015_02375"
     CDS             complement(<35743..36233)
                     /gene="guaA"
                     /locus_tag="PK015_02375"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOL33353.1"
                     /translation="MHYDTVLILDFGSQYTQLIGRVIRSFNVYCEIHPYHLTETRIKQ
                     INPKAIILSGSPFSINQEDAPQPDSSIWEMNIPILGICYGMQLIVSHFGGKVQHQTKR
                     EYGQAILQIKKSPPLLEGLALEEKVWMSHGDTISELPPEFIVLASTSSAPYAVIAHKN
                     LPI"
     gene            complement(36238..36864)
                     /locus_tag="PK015_02380"
     CDS             complement(36238..36864)
                     /locus_tag="PK015_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOL33354.1"
                     /translation="MLKIEGYILLPDFETNTWLLWDEESREAILVDPAAPSPDLLKRI
                     EELKLKVKYIINTHGHMDHIGGNSWFKNHLSAPLMIHSADAPMLVNSAMNLSLYVGNP
                     VSSAPPDVLLEDGMELSLGKYPVKVIHTPGHTPGGICLLADKFLISGDTLFEQSIGRT
                     DLQGGNHQQIINSIKNKLFVLPDDVIVFPGHGPRTSIGMEKKNNPFVS"
     gene            complement(36875..37852)
                     /locus_tag="PK015_02385"
     CDS             complement(36875..37852)
                     /locus_tag="PK015_02385"
                     /inference="COORDINATES: protein motif:HMM:NF014610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridylate)-methyltransferase"
                     /protein_id="HOL33355.1"
                     /translation="MPQRAIALFSGGLDSVLAVFWMQKLGFEVIPVFFSAAYIFPDKA
                     LKVAEENGLKLEVRDISEKHLEMLRHPKRGYGKNLNPCIDCHSLMIQEAALMLPELGA
                     DFIITGEVLGQRPMSQNLDSLKAVDKMSGAGDLVIRPLSQKLLPDTKPIREGWVNKEQ
                     MLDISGRGRSRQLQLAEQFGISHLQPAGGCLLTDHNYSLRLRDLMIHNQDILPNVKLL
                     RWGRHFRLTDEIKLIVGRDAADNAGIESENYPGLYLKIRDVEGPLGLLTVQEPSEEVL
                     KLAASIVLFYSHKASSPAYIKYGVDKKFNQEIKVEKCPENILRSYLITS"
     gene            complement(37863..38666)
                     /locus_tag="PK015_02390"
     CDS             complement(37863..38666)
                     /locus_tag="PK015_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33356.1"
                     /translation="MRHYLYIPSMLLLTFLILGGLSCSKEETPTTPNFEEYGISSHIW
                     DYNYVVWFDLDYTAQSKNMVAADIWMTAKGNASDASLKIGPTDIVFNNIESYTDGKIY
                     RGATINLSTDQPISYKITNLANNKTYTGSINFLPKNIVVDPWPSFSQDYNYSPLWSIA
                     ENPDFNVIDAGWEGRSQHCEYIRQIDGNINTYTLVKTEWQNQTPIENFYFGINAIGYQ
                     RKHDNKVLIVGVSNNYYWWNANGKGQNEWQAHRSPLKFMDLIQEDINSK"
     gene            complement(38786..38861)
                     /locus_tag="PK015_02395"
     tRNA            complement(38786..38861)
                     /locus_tag="PK015_02395"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38826..38828),aa:Thr,seq:cgt)
     gene            39586..>40365
                     /locus_tag="PK015_02400"
     CDS             39586..>40365
                     /locus_tag="PK015_02400"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HOL33357.1"
                     /translation="MKRTFLIVLLLLSCVFIFAQLSPWLWAQKAGGTGEDSGHSIAVD
                     ASGNSYVTGGFEGTAIFGSTTLESSGSQDIFVTKLDNRGNWLWAKKAGGTDSDYGYGI
                     AVDASGNSYVIGCFYSATATFGSTTLTGNGYNNIFVAKLDSSGNWLWAKNAKESPYDY
                     GHGIDVDASGNSYVTGYFYNSVTFGSTTLTSSGGADIFVAKLDSSGNWLWANNAGGTS
                     DECGNGIAVDDSGNSYVTGYFYDSATFSSTTLTNNGVYDIFI"
BASE COUNT        12959 a         7183 c         7245 g        12778 t
ORIGIN      
        1 tggtatgaac tgagttttta taatcttcat agtctaacct tcccaaaact tatcggtaga
       61 tataattgga atcttgcgac agatgatatg gtaatacaga atggatattt gtataccagt
      121 aatggatatt aaggatagct aactaagtat caataagaaa aagggtactc ttttacacaa
      181 aaataaagcg ggaaaaattt tcccgcttta ttaatatatt tcttaccaat tattttagtt
      241 cataatagct ttcacatcat catctggatt agatataggt tgtaaaccga aagtatctac
      301 tatgactttt gctacattag gtgataaaaa tgctggaaga gttggtccta agcggatatt
      361 tttgaatccg agagaaagaa gagcaagaag tacagctacc gctttctgtt cataccaggc
      421 aatatcaaaa gcgataggta attgatttat atcttccagc ccaaaagctt ctttcaattt
      481 taaagctata actgcaagag aataggaatc attgcattgt ccagcatcca gaactctcgg
      541 aataccgtct atttcaccta aattgagctt tatataacga tatttggcac atccagcggt
      601 aagtattacg gcatcagaag gaagtttctt tgccacttcc gtgtaatatt gacgacttgg
      661 cattcttcca tcacatcccg ccataaccac aaacctttta atcgctcccg atttaaccgc
      721 ttcaataatt ttatctgcca tagaaagcac agttctataa ttaaaaccac ctacaatggt
      781 tcccttttct atttcagtgg gaggtaggca tttttttgct tgggcaatga tagcactgaa
      841 atctttagct ttacctggtt ctctatctgg aatatgagtt gctcctggat atgctgtcat
      901 acctgtagta taaagacggt ttaaataggt gttatctttt cttaaaggca ccagacaatt
      961 tgtagtcatt agaataggtc cattgaactt ttcaaattca tccagttgat gccaccagga
     1021 agagccataa tttccatata aatggctgta ttttttaaaa gcaggatagt agtttgctgg
     1081 aagcatttca ccatgagtgt aaatatcaat tccagttcct tcactctgtt ctaaaagttc
     1141 tgccaaatct ttcaaatcat gtccactaac cagaattcca ggacgatttc ctactccaag
     1201 attaaccgtt gtaggttcag gtactccata agtttcagta ttggcacggt ccaaaagttc
     1261 catcacttta acggcatatt cacctgtctt aatgaccaga ttagttaact gctcaggact
     1321 taaattatcg tcaagggtag cagctaaagc tttataaata aattgattga ggtcatcatc
     1381 ataatatcca agcatagcac tatgatcacc ataagcagat attcctttta gaccataaat
     1441 tatcgtttcc cgcaaagaac gaatatcctc attttcagag gaaagcactc caacttgagt
     1501 tccaaaggat tcatattctt ctaaagaaag gtgaaagagg gcagcatcag gatattttat
     1561 attcctatct tttgctactt cttccagctc agcttttctt ttatctctta actttataat
     1621 atttgcaata gaaagcttga tttgctcttc atcccaattt gtattggtaa tggttcggaa
     1681 caaaccttgc ataatgatga gagcatcttc tctatgatag atgttatgct ctaataactt
     1741 ggatgataca aaagtcagac ctttcattgc ataaatagaa aggtccatta aatgacacag
     1801 ttcgggagat tttccgcaaa ctccacgcac agtgcatcct tgatttcttg atgtttcctg
     1861 acattgataa cagaacatac tcatataata actcctttaa tatgtttatg gtttgggcat
     1921 tttaataacc agtaatctgg caggtttatc actattatta attattccat gaggaacatt
     1981 tttggggcta tcaataatag tatcgggtgc aaaactttgt tcttcatctc caatgatgat
     2041 tgttggattg ccttccaaga tgtagaaagc tacattaaca ggtgtattat gaagcggtaa
     2101 ttttgctcca gcttccagat acatatggac aatttctcct tcaggctgag aataaatctt
     2161 agttccccga actccatcct tatctcttac gaggggcaaa tctttccaga aacggctttc
     2221 cataatttcc tcctttatat tttcttctct gcaataagat tttttatggt ctgaaaaagg
     2281 ctatcgcctt ctttccctaa atcaaacttt ctcccactaa gattccattg agtgataaga
     2341 aaccgcatag aaccaataag aatggtgaaa atttgttcgg aattaattcc tgattggata
     2401 aatccttcct tttgggcttc ttcaataaaa tttagtagaa cttctctatg attcatagat
     2461 atcttttgaa attgtttaaa aaagacaggg tcataataga acatctcatc cgagaacata
     2521 acttgtgcta aatctggatt agaagtgaaa agctcatagc ggttcataat aaaaaagtga
     2581 actttctcca gaggattgag cttagttttc tgaagttcct gtaatacatt ccgggaaata
     2641 tcttcaaaat agtgcataat gcttaagagt agatcttctt tattaacaaa gtgacggtat
     2701 aaagcagctt ctgatagatg tagttcagaa gcgagttttt tgatagtaag atttttatat
     2761 ccttctctgg caataagcgc tatagctgtg agaataatct gttcctgtct ttcagtcatt
     2821 ttttctcctg gatgtaagta aatgtttact aacctattta tttaaacctg attttttggc
     2881 aagaaaaaac cgcagctcat tttcttgctg cggttagtga aatgtactat gaaacgacga
     2941 ttattaattg gtagcgataa tcttgaagaa agctttagcc tcagttgcta tggtttgata
     3001 ttgagtggaa tacacggtat caatatagtt ataatctcca taaggctgag aagacttata
     3061 gattttatag cttgtagcat taggtacagc ttcccaggat agtatcagct gatttgttgc
     3121 attatccaaa gttatagtaa ctacaggagt ttgcagcaag gatttaactt ctacattatt
     3181 aatcataaaa tcattatcag catttgagac agttgagcca gcaaagaaag caatgcgttt
     3241 tcttccgcta taacctgcta gaggaatact gatatgagta ccactttctg gaatatcatt
     3301 aagcacaaag ggagaagaat cattattcca ttctcttagg atattagctg ttgaccagga
     3361 aaagccatct ccaataagga cagcaaacct atcatctata cctgttgtat catgaggagt
     3421 tcccgttgga ggttgattat acttcaaaag agcagcatca aagcttagaa cttggtcatc
     3481 gcttatatat aataaaggtg aaataagcca gccactaata ggaccccaga tattaatttt
     3541 agcagcttta tcagttgaag taatatttaa ccaatcatct tgttgccatt gacttgaacc
     3601 attagctcct aaaacaatag ggtctactaa tccgccagaa tggtcggtcc aaccagtagg
     3661 tttaaaagta ccatcaaaag tttctaacca ggggaaagtg taaatagtag gatctgccat
     3721 cgtagtaaaa ctccagatgg gacaatctat agcatagcca aaatcattat gaggaaccac
     3781 tctccagtaa taggtagtac cataatccag ataagttgaa ggagtatagt attcttcata
     3841 aacaatctct ccattaacca gatttgaagg aggattattt gagcctagcc agagacgata
     3901 atgatctggg cgaccgccca tattgttcca gcgaagggtg atattcacat tatgcccatc
     3961 actttcattg ggaggaaata cacaagctgc tgcaggtggt atatctgtaa ccggagtctc
     4021 aatccagata ttaggatgat aatttgacct ttgtccacta ccagttggaa atgatgcatc
     4081 ctgatgaata taaacagaac tattgcttaa agccgtatag taaaaactgg gtggacctcc
     4141 aattttactt ccatcatgat tttcccataa gatttcaaat ccataactgg aatcataatt
     4201 gaaaggtgtg gtgaattgaa tatgaaccca accaggacca ttgaaggtta caggaccgtt
     4261 atatacatta gtataataag aattattggg ataacctgcg gtggctgaag tgtaacttgt
     4321 gctataaaaa tgagccagat aaatttgctg attatccata gtataatttg atatcgaatt
     4381 actgacttta aaagctattc cagtgatttg aactgtacca ctcatccccg caccagtagt
     4441 ttcagtagaa gtatagaaga acttactcca tccataatta ttataaccat agacaggaac
     4501 tttattttga ttagtagttc ctgagccaat aaaataatct cctgcgttta gtgcaacaca
     4561 gacaaccagt agaaatagga taaagatatt tcttttcatt tccttaacct ctcaaggttt
     4621 tatttaatca gcatcatctt atgctgcttg attttattgc catcatccaa acgaactata
     4681 tacatagcgc tggaaacgat ttgaccatta tcatctttcc catcccagat gcagttgtga
     4741 attcctgcag agagattatc atttagtaag gttttaatct tctgtcctcg gacattatag
     4801 atatccaact tgactttagc atttttagct aaagtaaaag agattgttgt ttcaggattg
     4861 aagggattgg gataatttcc gtttattttt gtttctacct gaggaatatt ataatcttca
     4921 tttgcatctg gactaatacc aaaccactgc attaaatcgg acatcaattc atatctttga
     4981 gcttcctgat aaacctggga aaaatcaaag ctgaaataaa aagccttata gataggtaag
     5041 gtaactgtgg ttgcgcaaac tccatgatag agataagcat gaccatctat tggtccatct
     5101 tcaaacagaa atgcactttg agcaccgatt tctggatact gcacttctga ggcataccag
     5161 tctggacagc tatcataacg gaagatacag tcaggactat cagcataaac ttgatactca
     5221 tttcccacta ttcctatagg ggaataagtg taacaaagta tacttccata ttgataggaa
     5281 aagacttcag gtcctgctaa gccattagtc ccaccacctg aaccagttgg aacataatag
     5341 gaacggaaat aagcattaaa aagctttttg attaaactgg tattaggatt agcatgagtg
     5401 ctaccagcaa aaccatctga agcaaaaaat acatttttgg gttctgtagt agtcccacta
     5461 tccatatatt gcattaaagc taaacttaaa gtatctgaaa gggcagtgtg acttcccaca
     5521 ttggcataac acagaatagc tttgtattga agagggaaac ggaaactatc atattcttcc
     5581 caattataga tgtcatatga aatattgaga ttatctaata gattggtata gacaggaagt
     5641 tctattcttt gataatcttg acgaccggaa taagcaatca gaacttcggt atcagcagtt
     5701 ggtaatattt taaaactata gaaatgcgtt ggagcatctt caggaaggga tccggtatta
     5761 ccttcagaat ctgtggcagt aaaataataa ttcaattccg caccttgagg caactctggt
     5821 aactggaaat aatataaagt tccggattga ctcgtatatt ccatactttg ccatccttct
     5881 cccgtattat aatgcaaaac aagagaggtt attggagaaa ggtctatggc attaactttt
     5941 aaaagtggag ttctattgaa ggtattccct gcttcggtat aagttaaatg aggaggttgt
     6001 tcatctattc cagtaaagaa tttaatagca aggttatcat gaagcaggtt tccgggaggt
     6061 tccactcctt gatactgatt atttatcaca atctctctta aatagcaaag tcctatagtt
     6121 gcatcttgat tctggatagc tactgtggca ctctgtccat tttgatgagg gatattagag
     6181 ccactttgac ctgtatgagt ggtgtgatat tgcattagta tatcaccgtt ttcatagaat
     6241 atgacttcaa actttaactg aggagttcct ccggttcctg ccagaaaacg aagattattc
     6301 cattgtacta cagtataacg gttcggagct tctccaaagg tttgaaaata gatatcactt
     6361 attccctgtt ctgcaaataa atcatcccaa tatacggcaa ttaaagtggg cattgcatta
     6421 tagccaggaa tgggatacgc ataattaaac atattaccat cactatccca tccagtgtta
     6481 ggataaggtt tttgccagtt gctttcggaa agtaatatct ctccattgat atctacatac
     6541 atattatcat atagctgacc gtagaatggg aaaggaaatc ccataggaat agcttcacta
     6601 aagttatcat ctccttccca gctgggaacc tgatacatta tagttgatgt cccagtagaa
     6661 ctaatatcta tccaattaaa ttctggccct gcttcatcat tactatcaat aaatctataa
     6721 ccaaaagtgt ctggaccacc ctgagtgatt ctatttacta cagtaaatga ttcttccatt
     6781 gtattattag caggattgcc gtcttctgaa attaaactaa agatggcatt atagttccct
     6841 tctgaagtta taacacaaga ggttgagagg gtaatggtaa cgctatttcc aggttgtaaa
     6901 tcaagaagat gaaattcttc attactataa atagaagttc ctgaactatt taaaatatta
     6961 cacgaaatag aaagcattcc tgaataggaa gcagtaccca aattttgtac ttttaaggca
     7021 ggaataaaag aatgattctg atattcaaat tcacgaggag atggtatttc tataatacca
     7081 agatctatgg tcggcacagg aagaacttct actactacag tatctgaagc ggaaagacca
     7141 gagatattat cagtcattgt gaaagtaata ttttctgaac ccgtgaaatc aggagaacta
     7201 aaagtaacta ttctcccgtt tatctgaaca ttaatattaa cattgccaga aacactcaag
     7261 gtgcaagaat ttatatcggt gcaggttata aagggagtaa aatctaccgt caaagtttca
     7321 ccctggataa aactaaaact ttctggcaaa ttaagtgcag gttgaattat tagtggttgc
     7381 ccaatttgaa catcatctat caaaatccca taatttatgc aattccatag aaaaccaata
     7441 taaataatct gatttgccca gtttgaaaga tttaatgaaa ctctctgata ggtagttcct
     7501 atattttgta aatgaacaag ctgtgtggta aaattgttta tagctgttcc cgtggttgag
     7561 acataaactt tgagttgttc cgtacttatc caggaacgag tgtaaaatat caaagaatca
     7621 ccttcagcga tgtttaactg tggagtaatt aaccaatctg cattttgatt aggcaaataa
     7681 ttatcacaaa aagctgctct ggttccggaa tgggcatgag ttgcattatt aagattacgc
     7741 cagaccattc catctccatc atcatgaata gtccaacctg caggaagagt agtgatatta
     7801 tcaaagtttt catctatatg ccattgtcca agacagatag tgaaaatgct caaaagagcg
     7861 ataattaagg caaggtgttt gtacataata ttcctctttt tataaataag aaagttgtgg
     7921 attttctatc tgaatatacc attcattttc acctgctaca ttgccaccaa gggatatatt
     7981 ctctccccaa tagacagtga gcagaccctt acatttatgt ttataggaag aatcaatatt
     8041 ggttaaatgg acattctcat tagtccctaa acataccaag gcgacggtag caccatcctt
     8101 ccctccggta ataatagctt cctgcaagag tgcctcattg gattccgctt taaactccgc
     8161 tatcttatta ttttcaaaac gaatccaggg tctttctaca actttacctg atatataagc
     8221 taaatctccg gttatttctc catttaagct atcaggttct ggaatgagaa gaatctctcc
     8281 tccaggtaaa ttgattgttg gactctgaaa ttcactatct ccaaaagaac cacattctgc
     8341 cagaatcttc tgaatggtaa aactaaaacc tgtatccgaa ttgctaactt gtatagcatt
     8401 atgcttggac atttctaaac aaagattgtg agtgcgttga tagagcttat cataatctat
     8461 atccaaagct ttccagaatt gtatttcact ttctatggta ttctcttcca gagtgtttgg
     8521 aagcaaacca ggcattatag cgattttaat cttattctgt tggatgattt ttcggctctg
     8581 agcaaagtgc ttgctacgta atctctgttt ttgcgggtca ttaagttcta tataacgagc
     8641 ttcaccatta agatttagat agacattatg tttagctaaa agacgattga attgtttata
     8701 atcagcatca aaagcagata aagaatattc ttcatatttc gcttttaata tctcggttga
     8761 ttgataaatc caggtagtat tgatactcct tcgtcctgct tctactaata ttttttctac
     8821 taatggttga ttatagcttc cgcttacgat aagaagagat tcctgaggct tcatttttag
     8881 actgtcttct aaaagttttg ccgcaatact attaagattg atatgatatt cctgggtcaa
     8941 tatattatct attatatccc ttttactcag agaatggtca ttaatatttt gaccaatagc
     9001 aaaatctaat atgtaattct ttgccgtagc acgataatat acctctttcg ctcttacttg
     9061 attggaaggc actatctcta ttaatttttc atctaagagt ttattgaggt tgtagtgcac
     9121 tttttgttta ctaataccat aaatatctgc cagttgctga caagtagcag ctccacgaat
     9181 gagctctctt aaaatattat atctcaattc ggaaccaagt tctctaattt gagagtgctg
     9241 atttaatact gcgatatctt ttatcatatt ttcaccttac aatttcactt aaaataaaag
     9301 ccagctattt gtcaagatta ttttgaccaa tcctatactt ttcttaaatt atctcacctt
     9361 aaactgctct ctaaatttcc caacaatccc tttacaaaat ttcttttaac tctaataaga
     9421 attatattat ctttgtttaa taaaaaaacc taacgctata tgaaactaag gagtaaatat
     9481 gcgacctaat agacaggaag tgattgaaat gctgtcttta aaaaagaaag atgaaataga
     9541 agcattatat aaaagagctt atgaagtgaa aaagcagtat gttggtacaa aggtatactt
     9601 acgcggcttg atagaattaa gtaatatctg taccagaaat tgttattatt gcggcattag
     9661 aaaagataat catcttctgg aacgctatta tattgataaa gaagaggtat taaaagaggc
     9721 aagatggtgt tttgaacagc attatggttc tttaaccatt caagcaggag agcgttcaga
     9781 taagagctgg atagattata tagaggaact gattaaggag ataaagacat taacggatgg
     9841 gaaaatgggc attactcttt ctttaggaga acaatcagaa gaaacctatc gtaggtggtt
     9901 tgaggcaggt gcacatcgtt atctcttgcg tattgaaacc tcaaatccag aattatataa
     9961 aaatcttcat ccagcggaga attcatttga aaatagattg gaatgtatcc gcagtttaaa
    10021 gaaaattggt tatcaggtag gaagtggagt aatgataggg cttcccaatc aaacggtgga
    10081 ggatttagcg gatgatatcc ttttttttta tgatgaagat atagatatga ttggaatggg
    10141 tcctttcatt cctcatcatc aaacaccttt ggcatattta ttggcaactt tccatccaga
    10201 ggaggcattg gagcaagctt tgaaaatgat agctgctaca cgaattgccc ttaaagatgt
    10261 caatattgca tcaactacag ctctacaagc attacatcca aaaggtagag aacttggact
    10321 tctggcaggt gcgaatgttt taatgcctaa tataactgat actcgtttcc gagagggtta
    10381 tcagctttat gaagggaaac ctggtttgaa tgagaatgca ttagcaatta gaaaagctct
    10441 ggaagaaagt attttttcca taggggagag cattggatat gatgaatggg gcgattcacc
    10501 tcattttaaa agcaaaaaaa atacaccttc tgagaatcca taaaattaga tatcccttga
    10561 cgaaaaaagc ccttatcctc tttagtatat catattatct attttttaga aggaagaagc
    10621 aatgcagaat atgaatcaaa ttatgaaaca gatgcaaaat atgcaaaaag aaatgatgaa
    10681 atcccaagaa gagctggaag gaaagatttt tgaagttact accggcgggg gaatgataaa
    10741 attggaaatg aatggaaagt atgaggtcaa atccataaag attaatccgg aagcagtaga
    10801 tccagatgat gtggaaatgc tggaagacct tatcctttcg gctttgcagg aagcacataa
    10861 acaggtctct gaagccttag atgaaattat gggtaaatta actggaggga tgaagattcc
    10921 cggtatcttt taaatgatta tatcacctgc tttagaaaaa ttaatccagg cattaacccg
    10981 tttccctgga ataggaaaaa agactgctca aagaatggct tggtatctgg tcagtcaaga
    11041 taagtctttt ggcttggaat tagctgaaat tattaagcag acggtagaaa acattaccac
    11101 ctgttctctt tgtaatatgc tttcagaaag tgatccctgt cctttttgca cttcttccga
    11161 aaggacggat tctttgctct gtgtggtgga gtcctctgct gatattcaga taatagaaaa
    11221 tatgaatgaa tataaaggac gctattttgt tctgggacat ttactttctc ctattgatgg
    11281 ttatggtcca gagcagatac attcagccca atttctccaa gctatagagc gttttcatcc
    11341 tcaagaagtt attctggctt taaaaccttc tcccgaagga gaagccacta ttcattatct
    11401 ctgggaattg ttaaaagata aagatatcaa agtgacccgt ctttctacag ggcttccttt
    11461 tgggggagat ttggaataca gtagtatgtt aactctttcc aatgcctgga aaaggcgtta
    11521 tccggtttaa tatgtttact ggtattgttg aaactacctc tcgcgtgatg cgaaccctga
    11581 acagttctgg gctaaaagca ataaccatag aacttccgaa atcatttaat gatttaaaaa
    11641 aaggttccag catagcttgt gatgggattt gcttaactgt cactcaattg aatacggatt
    11701 cctttaatgt agaaattatg caggaaacgg tgcaaaagac taccgctgga aattggaata
    11761 agggaactat cctgaacctg gaaagagcat tgaaattgga agaccatctg gatggtcatt
    11821 tagtgcttgg acatatagat agaaccacca cacttttaga gaaaaaaact gtttctgcca
    11881 ccacttattt aagattttct ttagattcca gagaccgtga acttgtggtt aagcagggtt
    11941 cagttgccct aaatggagtc agtttaactg tagcagagct aaattccagc tcatttacgg
    12001 ttgctctcat tagttatact ttagatacca ctaacttacg ttatctttct ccaggtgcaa
    12061 atgtaaatct ggagtatgat atcataggta agtatatcca gaatctttac catagctctg
    12121 gttttaagaa caaactatga aaaaaatctt taaattaagc ataattcttt gcctggtcat
    12181 tttcctggct tcctgtggta gtaaaaaaag tcctactggc ggtcctgaag acctgtcaaa
    12241 accggttgtg atatcatctt taccaccaca atttggtaat atatctgagg gacggattga
    12301 gattaacttt tcaaaaccac tggataaaac ctctattccg cattctgtat atatctatcc
    12361 acctgtgatg aataagaaaa ttaccgttga taaaaactct gttatcatcc agataaatga
    12421 aaatctccta cccaatacaa attattatgt aattattagc accaggctaa aagatatacg
    12481 aggtaattca ccggatgaga atcaaactct ggtctttgcc agtggcaagc tcaaccagct
    12541 tcgtttatcg ggaaccatag attatgaaca tccaggagac aattctttac cagttcagat
    12601 gagtttacta tcagaagatt ctcttttagt cctatctcag gtggcaagag gtccttctta
    12661 tgccatagaa ccactcaatc ctgctcatta tatccttcgt gcctatatag acaagaactt
    12721 gaatggacgc tatgatttta ctcaggaacc ttattttgag caacccgtta gggtagagca
    12781 tcctactaat ctaaacctct ttttagctta tgctgatacc accaaaccta atattaaact
    12841 cgtgaccgca caacataaca gagaaattga aataactttc acggaagcaa taaaaggtta
    12901 ccgttcaatc aaaatcaatc gtctggataa taaggaagaa ttacctgtct tgctttctta
    12961 tctggaagaa gataaactta ctttgctcac agcttctcag gatgcttttg cttacacctt
    13021 ggaactatat ggagtagaag ataagaaagg aaatgtaact ccagtgatta agtcggaatt
    13081 tcgctcttct acgattatgg atacgcaacc acctcaggta gtatatagct cacctcgtaa
    13141 tggaacttct gtaaaatcat tagagcccat tctggaagtt tgtttttctg aagttattcc
    13201 ccgagcttcc gtaaaagcct ctttaagagc ttctgaaaca aaaacagaaa tccctcttga
    13261 ggtcctgcag tcagatagta ggatttatcg ttttaagccc caaaaacctc ttcaaaatta
    13321 taggtcttat gtcttgacta tttctgcaac ggatatcagc ggaaatagta tgccggaaag
    13381 ttttaatctt aactttctac cacttatata tcaagactaa agacgaaaga accgtaaagt
    13441 tagagttcaa gcacttcctg tattcggtgg tgaacttctt ttagaccaag tatggaaaat
    13501 atagcgggaa gttcaggtcc gtgatcttga tgtagtagtg ccagatagag aggtttataa
    13561 aaagctttac ctttaaagcc taaatccttt attccttgtt gaacaagctg gtttagctgt
    13621 tcagcatcaa tatctcctcc agaaggaagg tgactttgaa accaggacag cactttccga
    13681 gaatcttctt ttttaagaaa atccaaagat tctgtactta ggggttgagg acagaggaac
    13741 attttagaat attgaactag gtccgaaagt aaagtgcatc tttcccttgc agtagcaact
    13801 attttagtaa gtttatcaga atcgggaata gcgagatgct ctttttcaaa gtatggcttt
    13861 gaccgttcca ctatagtatc cagaggaaga ttacgaagat accaaccatt catccagtta
    13921 agcttgttca agtcaaagat agcaccggat ttattaactc tttccagagt gaattcctga
    13981 caaagttcct ccagactgaa tatttcacga ttatcagcac tatgccatcc gagtaaagca
    14041 acaaaattga ttattgcttc ttttagatat cctctttcca ggtaatcttc tacggctaca
    14101 tcattcatcc tcttacttaa ctttgtacgg tctggattaa gaattaatgg aagatgcacc
    14161 cattgagggg gagtccaacc aaaacactca tataaccata gatgttttgg catactggaa
    14221 agccattctt ctcctcttat cacatatgat atatccatat aatggtcatc taccaccgag
    14281 gctaaatgat aggtgggata accgtcagat ttgataagaa cttggtcatc tgactgagca
    14341 gaatccattt caacttcacc tcggataata tcattaaagc gaaaagtcct tatttcaggc
    14401 atttttaggc gcagaacata aggttctcct ttagctaatt tagcttcaat ctctgccggg
    14461 gtgagattta gacaacgacg gtcatattta acaaattcac ctttagcctg ttgttctgct
    14521 ctcatttggg ctaaggtttc aggagtgcaa aaacaccgat aagcaaaacc tgcatccaga
    14581 agtttttgtg cttccttctt gtataaatcc aatctttcac tttgaatata aggaccatag
    14641 ttccctccaa tgataggacc ttcatcaaaa gttatatcaa gtttctgtaa ggtatggata
    14701 aggttttcca tggcatctgg aactatcctt gttctatcag tatcttcaat tctcaggata
    14761 aaagttccac ctgtgtgacg agcaaataga tagttatata aggcggtacg aagacctcct
    14821 atatgtaaat aaccggttgg actgggggca aagcgaacac gaaccataat tttaactcca
    14881 aacttttatt aaaatataaa ccttaagaga gaatgatgga taactctata atgagggtca
    14941 taccattgac gatattcttc agaatgggtg ggcataatct ttttagttaa acaatcttct
    15001 atcatcaatc cttcttctgg ggttgcagga ataatggatt ttaccacagg tatacaattt
    15061 ttccaggtct caatccaatc attccaatta atctcatttt caaagcgcga ttgcaatata
    15121 caatctgcaa gaatcttaat tttatcttca gaatctttta aatactcggt tggtatcttt
    15181 ttatttaaca acattgaatt ttgttttagg tcctcatctg gcaagcttat tatagatatc
    15241 agattaagac gaataaatcc ttttccaaca ccaatatctt gatagggaac atccgttttc
    15301 cacttcatat cattaagttc tcgcttcaaa ttatagataa tagtgtctcg ctgaggttca
    15361 atatgagagg gaccataaaa ggcttgataa agtaatttga acacatcaag gtaatggcat
    15421 cgtggatgca acttaatttc ctgaaggata atatcgctta tctcatccaa agtaaaatct
    15481 ggtttaacga tcatcataaa cgatatttcc atcttttatt attaataaag tccgcacttt
    15541 gttccaatca aatagtttcg tgaaatccgt gtccataaca cttaaatctg caatatatcc
    15601 ttcttcaatg attcccagac tttcttctgt atgattcaac caggagttat tttcggtgta
    15661 actctttata gcttctatag gttctaactt ttcttgagga ttatgatgat ggataagagc
    15721 ataaagcgac atcgcaatat tcatatctgt cacataccaa tcgctggaac tgcaaatgga
    15781 tataccttgt ctggaaagtg attgaaaccg gttcattatc ttgtatcttt cacctaatct
    15841 ttcggaatat aatcccgaat ttccacccca aagtaaatca aaggcgggct gcattacagc
    15901 tactgctcca ctttttgcta tctcttgaat taaactatca ggagtaactt cacagtgaat
    15961 cagttcatct cgtaattctt taacctcttc tctatcttga gtggcataag cagaattgat
    16021 ttgatgaatt gctgcatcac cgatacaatg aactgctact tgcaaatgtt ccttatgagc
    16081 ttgagcaaca aattcctgcc agaattcatc attatgatat aaaatgcccc gggtattttt
    16141 atccttatag ggtaaagtca ttgccgcagt gtgagaacca atagaaccat ctgccagaat
    16201 gcatcctcca attctttttg ctccaagctc taaagcatca cggatattga aactctgagg
    16261 ataaagttca aagcttaccg ggaattcatt aagatggtca cgcacgagtt tatagtgttg
    16321 attactcatt tgagcatcgc caatcatagt atgaactgtg gtaaatccac cttttaaagc
    16381 agcttgagca gcattcttat aggcttcaat gatttcttct tcagaaagat tatcctgtaa
    16441 ccagttgact gctctatcat tatcttcagc aatcaggatt tcattggaac ttgttaaacc
    16501 aggaataatt gcccgagctt ttgaattcaa aacgcaggaa tgaccatcaa ttctacgcaa
    16561 tagtaaagga cagtctggac aaacagaatt cagctcttgc ctagtaggaa atcttttttc
    16621 tgctatcttt tcttcatcaa atcgccaagc aaagatatat tgctgattat tttttagaga
    16681 attagatata tgttctaaaa cctcttttat agatgtgcat tcagaaaggt caataccaga
    16741 aagatataac cctccagaaa aacaatgagt atgggcatca ataaaacccg gatagacata
    16801 agctccattc aggttaatct tttcccaatc aggatttact ttatcattat taaagcataa
    16861 ctttactatt ctatctttat ctatcagtaa acccgataat tgtgcatcaa atttagcatt
    16921 atgataaatg tgtgcattat aaaggtaaat catataatta accaggatta tctatcttat
    16981 tactttcaaa tatagaagat aacatacggt aaaataaatt aataggaaaa cccataacat
    17041 tatagtaaca tcctgttatt cgcttaataa attgtgaacc aaaaccttga atcccataag
    17101 ctccagcttt atccataggt tcacccgtgg caatataagc atcaatatca gtttcggtta
    17161 ggggagagaa ttctaccaga cttctttcat aattgatagc ttgaagggtt ttccagcaga
    17221 tgcatacacc tgtgtagacc ttatgcgttt ttccggaaag cagagtgagg aattcttttg
    17281 cttgcatcac attttctggt ttacctaaaa ttttattttc cagaactacg atagtatctg
    17341 ctcctactac aacagtttca ctatctatta aagcagcaat ggttgaagct ttgttttttg
    17401 cattatgcat cacttgtata taaggctttt caccatttat agtttcttca attttagctg
    17461 gaataaccag aggttgcagt cctatcattt tgaacaattc taatcttctg ggactgctgg
    17521 aggctaaaac cactttttta tcgttcaata agttgtgtat catagattat accttgaatc
    17581 ctggatagca atatagcgaa ataagtcaat taaaatcctt taaaagttaa aaaaatcctt
    17641 gcctgaaaaa gcagtattag aaatcctagt ttttagtaaa caataaatat tccaggagga
    17701 ataaaaatgg aacgcgtgta taacttcaac gcaggaccag cagtgcttcc cgaagaagta
    17761 ttgctggaag ctcaagctga tttgttcaat tacaaaggta tgggactttc tgtaatggaa
    17821 atgagtcatc gtagtaagga atttcaagcc attatagacg atgcctatgc cagagtgaaa
    17881 cggatttatg gcttgggtga tgactatgag gtccttttcc tgcaaggagg tgccagtctc
    17941 caatttctaa tggtgccctt gaattttgca gtggaaggga aagaagcaaa ctacatcaat
    18001 actggacaat ggtctaagaa agcaattgca gaagccaaaa agattggtaa ggcggttcat
    18061 attgctgcca gcagtgaaga cagaaatttt gcctatattc ctcggtcctg gcaactttct
    18121 gataatcctt cttatctgca tattaccaca aataacacta tctttggcac ggaatggaag
    18181 tttgatccag aaattccaga ggacatccct ttgattgctg atatgtcatc taactttatg
    18241 agtaaaccca ttgatatcca gaaatattct cttgtttatg ctggtgctca gaagaatgtc
    18301 ggtcctgctg gtacagttgt cgttattgtc aaaaagagtc ttttagagaa agcaattcct
    18361 ggtttaccaa caatgctgga ttataacatc catgcaaaaa atggttcact ttataatact
    18421 cctgcctgtt tcatcattta tatggttggg ttagtgttga agtggattga gaactttggc
    18481 ggtctggaga aaatagctga aaataataag ttgaaagccg gatacattta tgataccata
    18541 gatgaatcta atggtttcta tcgtggaact gtggataaag aagaccgttc tttaatgaat
    18601 atcactttcc gtcttcccag tgaggaattg gaagaactgt ttatttccga agccaagaag
    18661 aatggaatga taggcttgaa aggtcataga gatgtgggcg gatgccgtgc cagttgttat
    18721 aacagcttac ctcttaaagc ggcaaaaaca ttaagcgaat ttatgaaaga atttcaacgg
    18781 caacacggtt gaaaatcact ataatttata atcaaacaat aaagaaggcg ggagattctc
    18841 tctcccgcct ttcccatgag aggaaataat tatatgtctt tactggattt cataatgcgt
    18901 ggcggatttc tgatgtatgt gctggtggct atttcagttg ttattattgc cattgtaatt
    18961 gaaaaatact atcagattca taaagtgaga aaggctaatg aaaaactatc tttttatctg
    19021 gctcaacaag ataaaccgga aaatattaga gctattctga gaatacatgg acagagcagt
    19081 cctcttggat taatgctgga caagctttac aatgctgata ctgatgatat taaactgatt
    19141 caggaaagta tggaagctgt aggtaatcgg gaacttcata atctggaaaa aggaatggac
    19201 tggctttcta ctctttccac tattgctcct ttagtcggat tcttagggac agtgattggt
    19261 atggttcagg tttttatgaa tatccaggga cagggacaaa atacggtgga tataagtact
    19321 ttggctggag gtatctgggt tgctttatta actacagttg gtggtttagt agtggggata
    19381 ccgtctatta ttttctataa taatttagta caaaaattgg atgacctggc aaaagatatg
    19441 cagcacgaag ttatggaaca cgaaataaga tatcgtaaaa tgctggaaaa ggcttaatat
    19501 tatgcgactg gaaatttcca aaaagaaaat tagcagcacc actctggttt ctatgacgga
    19561 tatgatgttc ttgctactta tctttatggt tctggcatca aattttaccg ctcagactgg
    19621 tttaccatta aaacttccag cttccgtatc acaagaacga caggtgcatc aagtgctcca
    19681 aattcaatat cttcccaata aaactattgt gtttcaaggt acagaatata gcttggaaaa
    19741 tcttggtcct gccttacaaa aggaattcag aagtaaagac caagtagtgc gtctttctgc
    19801 agaccgttcc acacctcttc aggatgtgat tacgataatg gatataatcc gtggtgtggg
    19861 ctttgaaaga atattcgtag gtactgaacc gattaaggaa taatataatg acattaccga
    19921 gtggcaaata tagtccctgg ctaatttcta ttatcttgca tggtattttg ctgctcatat
    19981 ttagttttta ttatattaaa ccccaagtcg ctattcactg gtatcaaata gaaccttatg
    20041 accctttaac tcagtgggaa gaaactccag tcatcaagga tcaagcccga gaatctatta
    20101 aagaagaagt ctcaccatta cctcaaatca gcaataaagt aatagctcct caaaaatctg
    20161 aagttcctcc tcaaacttta gagcaagaaa ctatccctca acctttagca ggagaagtga
    20221 tggaggcacc tgaattatct actcaaccag ttcctaaaac cactgctcct gtagtaaaca
    20281 atcctttagc ttatggtttt ttaaaaggtt tgaccggagg agacaaaagt tcggcagcgg
    20341 gaacagttaa tgtctccttt ggtaaaggca agggaagagt ccgttttgat tttcctcaag
    20401 attatcgtca ttctttaggt gctccaggaa ccgttactgt tcgttttaaa gtagaccaat
    20461 atggtaaacc aataatgagt acagtggatg caattgagca gagtggacct cgttattttg
    20521 cggaagcaag aaaaattttg gaaatgtatc gggataaatt ccacattatt ggtgaaccac
    20581 aaccaggaat tgaatgtgaa ctcactttta tttttcagta atgatagaat ttaaaatacc
    20641 tgccttaatg ggaatattaa atattacaga agattctttt tctgatgggg gaaagtttat
    20701 aaatctaaag gatgctttgc ttcaggctga aaaactaatc tcggagggag ctgatatcct
    20761 ggatattggc gcagaatcta ctcgtccagg tgcagaacct gttcccgaag atatagaatt
    20821 gcaaagaata attcccgtac tcacggaaat tagaaataga tggcaggata tacctatttc
    20881 tatagataca cggaaaagta aggtagcaga agcagctata gatttaggag ccagaattat
    20941 aaatgatgtt tctgcccttc gttttgaccc tgaaatggct gatatgttat catctcatcc
    21001 tcaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnataggt gtttccagaa
    21121 aacgttttat agcttccttg gataattctt ctgtggagca aagattggga ggaactttag
    21181 ctgcgggatt ggtagcagtt ttaaaaggag tggagatact gcgagtgcat aatgtgcaag
    21241 ctcatcgtca attctttcag gtacttaagg aaataaatga ggttagtgaa tggacatcct
    21301 aatacctaaa tttgccgata ttgttgatat cttcattata gcttttctta tttatcagat
    21361 actcttggtc ttaagaagaa gtggaggttg gcaaattctc gtggctgtag tagctataat
    21421 catcttagct accattgcca cagctttaaa tcttcggatg gtttcttcac ttctcaattc
    21481 tattagtaga tactgggctc tggctttaat aattcttttt caacctgaaa tccgaactct
    21541 tttaagtaaa attaatcttt ctcgggaatt gaatctgagt cttagaaaga gagaaactaa
    21601 ctatgtatat atgcctctga ttgatgctat ttcctctctt tcttttcgta aaattggtgc
    21661 actcattgtt attgaaaata ggcggaaact aactgagtat atcaataatg gtgagataat
    21721 tgatgcagct ctttctttaa gattggtttt aactattttc aatcctaaat ctcctttaca
    21781 tgatggagca atcattatca gaggtgatcg tattattgct gccaaagtgg tgctgcctct
    21841 ttctaaaaat cctgaataca taaagcattt tggtacccgt catcttgctg gaattggaat
    21901 cactgaggtt tccgatgcta ttgctatcat tgtttcggaa cagaacagtc agatatcggt
    21961 ggctcaaaat ggaaaaatta aagttaatgt ggcatatgaa gaattgcttc agattcttat
    22021 ggatgcaact tcttgataaa atagaaaggt ataaagatgt ctcaacctat attgattgga
    22081 attaccggta acattggaag tggtaaaacc accttctgta atcttttaga agagaaagga
    22141 tttaaagtta tatacgcaga tgagattgca caacagcaat taaatcaacc cgatacttta
    22201 aagaccttga taaaaaggtg ggggaaaggt atagtaaaaa atggtcaacc tcagagagat
    22261 aaaattgccg aaattgtatt caataataaa tcagaacttg atttcctaaa tagtatagtc
    22321 catcccaaaa ccctttctgc tttacaacaa atagtggata atagcactga aaaatacctc
    22381 ttttttgaaa tacccttgct ctttgaagca ggattagaac tatgttttga ttatattatt
    22441 ctcattcgtg cacctcgtga agtccgtata aaccgattat tacaaaaggg aaaagaaaca
    22501 agagagcaga tagaagcacg catcaatgcc cagattgatg accaggataa aataccacta
    22561 tgtgatttag ttatagataa ttccaaagac ctcattgaat taaataatca gctaaattct
    22621 cttctttcaa ccttaaaaga cattaagcca aaggaaaaaa tacccttctc tttatgatat
    22681 tttacggtta attttatcta actgattaca atatcataga ccatcactta tttgacaaac
    22741 cgttaaaact cccttataat atactaagga tttaatatct cctgctataa aatatttaag
    22801 atatctaagt ataaagcaag aaagtagtga gatgtttatt atctaaagta aagagaatat
    22861 agagaaagag caatagaaaa gatgaaaaag ttttttcaga gaatagttga ttttctggga
    22921 atgaattcca gtctggcagg aatgctgttg atggtcattc tggtagggat gggagaaaag
    22981 atggcagaac gctttcttcc tctctatatt attgcgatgg gcggtactcc tattataatt
    23041 ggtctcttaa atggattgga caatcttctc agtgctttgt attcttatcc aggtggttgg
    23101 ttagcggaaa aattaggcta taaaaaagct ttagctatct tcaatttaat ggctatattc
    23161 ggatatttga ttgtagtttt gtttccaacc tggcaagcag tgctgattgg tgctatcttt
    23221 tttatctcct ggacagctat atccttacct gcaactatga gtttaatatc ttctgtagtg
    23281 cctgcaaatc gtcgttctat gggtgtctcc ttaaattcca tcgtaaaaag aattcctatg
    23341 gctctgggtc ctattatggg tggtgcactt attatgaaat ttggaaggat tcagggtgtc
    23401 cgttatgcct ttatagtagc cataatttta gccattgtag ctctctttgt tcaacagaag
    23461 ttaatcgttg aacctcccgt agagaaaaag acaaaacaga aaaaagggtc cagacgcttt
    23521 tctaaaaatc ttactaatct tctggtatct gatatcttag tgcgattctg tgaacagatt
    23581 ccttatgctt ttgtggtggt ttgggtggta gaaaataatc acataagcga aatgcaattt
    23641 ggcattttga ccgctattga aatggttaca gcaatgctta tttacattcc agttgcctat
    23701 ttagctgata gaagtacccg caaaccttat gtagctataa ctttcttcaa tttcactatt
    23761 ttcccaatag tcctgctctt tagtaaaacc tttccagctt taatcttcgc atttattata
    23821 agaggtctaa aagagtttgg ggaacccact cgcaaagctc ttattatgga cctggcacct
    23881 gaagattcaa aagcttggac ttttggcact tattatttca tccgggatgt cattgtatcc
    23941 gttgcagctt ttgccggagc ttttctttgg aaggtgagtc ctgcgctaaa tttcctgatt
    24001 gccgcgggtt ttggtctact gggaactctc tattttattt tgaaagtgca ctctactcct
    24061 gttcctcaga cagctattaa tccggaataa aactcaagtg tagatttata ctgatataaa
    24121 agaaccagtt ttaatattct ttatcccgta ttaacgatga tataattatg acgaataact
    24181 aatggataca attttattct tcaaaacgat agcagtaaat taaactcttt taaacttctg
    24241 tttggggaca aattatacat ttaattacat taaaattaga cagttatatg aatattatta
    24301 tcccaaactc ctaattgact ctctcttagc actttataag caaaagagct aagggagagc
    24361 taagagacaa tttgagttaa attatgctgg caatgtttcc cttgataaca atcatagaat
    24421 attataaaag ttggtagtta tttgtagtga ccattattgc actttacttt ctcggtttat
    24481 atatcgggtt gtgaattgta tactataacc tcctcaaagt ggtttttccc ctataagcta
    24541 aaattatcag ataaatataa ttaacttcac tgaagcttat aagatataga agtttctaaa
    24601 ataagtagtt taaaaaatct tgactgcaga aaaatgtctc caaaaatgag ggtatgaaaa
    24661 agataattca tattgatatg gatgcctatt ttgcttccat agaaattagg gaaaatcctt
    24721 ctctgaaagg aaaatgtgta atagttggag gattacctga tagtcgtggt gtggtagcaa
    24781 cctgttctta tgaagcaaga aaatatggta ttcatagtgg tatgagttcc tatcaagcct
    24841 ggaaattatg tccgcaagca gtttttgttc acggtcattt caagctttat aaagaggtct
    24901 cggaacaaat cagggctatt tttcatcaat ataccgattt agttgaacct atgtccttgg
    24961 atgaagctta tctggatgta actgaaaata aaatcaatga acccgatgca gtcaaaattg
    25021 cccgactaat aaaacaggat atctggaata ccactcaatt aacctgttca gccggtgttt
    25081 cttataataa atttcttgcc aaaatagctt ctgaactgca aaaacctgat ggtcttagtg
    25141 tcatcacacc agaaaatact cgtgaaatac ttttctcact gcctatagag aaattttatg
    25201 gtattggaaa ggtcactgct gcccgaatga agaaaatggg aataaaaaat ggagaagatt
    25261 tatataatca agatttaagc tttttaatca gcaattttgg taaggctgga gtttattatt
    25321 atcaggttgt tcgtggaatt gatgatagag aagttattac tgaatttgaa cccaaaacgg
    25381 taagctgtga aaatactttt gagaaagact tggatgacat caatgatatt ttagcagaat
    25441 tggaaaaatt gattgaacgt ttgataaatc gtcttgcctt tcaaggtata atggggaaaa
    25501 atatctttat caaaataaaa tatgataact ttgaggtcat aactcgttct tgtcctttac
    25561 ctgaacctac tgctgaccgc gctattcttt atgaatatgc agaaaaacta cttctttcta
    25621 actgggacca ttctcgtaag ataagattgt taggtgtagg tgttggtaaa cttgacttag
    25681 aaagaccaga aaaggaagaa caactggaaa ttcctatata aaattcttct attttagaac
    25741 tttagcagga cgagaagatt tctgccacca attacttaaa acaatcaaaa ccagaccaat
    25801 taaagtggca ggagaaatat gttctttcaa gataaagaag ataaagataa gtgatagaaa
    25861 aggagttaaa aagattaaat tagctgtaga agcagtgctt gtgctatatt ccaaagcttt
    25921 ttgccagaga agaaaagtga gacccatctc aaaaattccc acataaatcc ctcccagaat
    25981 gccaaatgtt atatttgcct cagaactaaa taaaccatta taattcaatt ttcccagtat
    26041 taatgcatag aaaaaagttg ccagagtacc aattaagaaa ttccccaaaa gttttttaat
    26101 agcgggaaga ctatctttca tatttaaaat ccaataaaaa gcccagacta aagatgtaga
    26161 aacagctaaa agagaaccca ccgggtcatc aaattgaaat tcccagaacc taccttgagt
    26221 tgagaccaga aggacaccaa agaaactaat gaaaagagcg atgatatctt taatcctgag
    26281 tttttgcttt aagaataggg cagaaagaaa agacagtact atagcccaag tataattaag
    26341 cacttgagct tcttgagctc taaggcgatg ataagcttca aaaagcatta tataataaat
    26401 aaagggattc aacaagccag atagaattcg tattttccag ttaaaagaag gtttatttgt
    26461 cttatctctt ttgtgattaa ccagaaagat aatagctaaa aatatagtag cacataagga
    26521 ggagattaaa agtaaaccca gcggagtgag atgttttaaa cttaatttaa atgctgtaga
    26581 aacagttgac caggcaagaa tagccgcaaa agcatagtaa taggcttttt gaaatgtttt
    26641 attcattaga ttaaaaaagc ctgctccttg aggaacaggc tttagattta tcagaagagt
    26701 ttatttcatc tgtttcagaa tattggtata ctctgtttcc agcgctttat ttttcatttt
    26761 ttgagcagct aagataacga attgaactgg catcttattt gtttcgtcaa acttatgcca
    26821 tttctgcgca taatttgcca gttcttccca tctttccata gaattcaaga ggataacgat
    26881 gttttcataa tagatattgt cttctttaat atcaagaagc tgttttagat aattcagagc
    26941 agcctcatca tcatccattt ttacagcaat atcataagct gtggctaaag catcggtgtt
    27001 tcgtggctct ataatcaaag ccaattgaat attgttttta gctttatcgt attgtccgct
    27061 ggcataataa caatctgtta aaaccagtaa attcttgata ttagtaggtt ctttttcttt
    27121 cactttttca aaaagaggga tagctttttc aaagtcttgt ttattataat aataagctcc
    27181 aagattcatt atcacttcaa cattttctgg attctgttca tataatttga aaagatattc
    27241 gcctgctttt tccatatcct taagttcatt ttggtagata ttgaccagca tattcaatgg
    27301 ttttaattct gtgggatcca tttctgcagc ttgttcaaaa atcttaattg cttgctgagt
    27361 gttgccagca ttcaattctt caattccttt attatacaca cgagtccagg cactaagttt
    27421 ccttttcttc atatctcgga ttgcagctaa ctcatcttcg gtaggctttt catatttttc
    27481 cataatcttt atggctctat cgtaatattc ataagccatt ttgttgtatt ccagagattt
    27541 atctggattc tgttcaccat aatagagata aatatctgcc acccgtctca aagctaaagc
    27601 atgatcagga ttatcctgta atacacgatt ataatatttg agggcttcct caacattttg
    27661 ttgagaatag tagacattag ctgtttttaa atctacatta gcttgtggac taagtctatg
    27721 tctggttgtc gcacaaccag ttaagaacag caaaacagct aaggtaagaa gaaagattcg
    27781 tttcatttaa atgactcctt ttcttatata tgcaatttaa ttaatacatt aatattaagc
    27841 aaggtgttaa gtcaaggatt tttccaatat gataagagag aatatcttag agcaggaaat
    27901 gtattatatt gtcttgcaag gaaattgata aatgaaaaaa gagtggataa aaaatcttga
    27961 ccaaaagcag ctttttaaat agaaagatac aaataaacct ttaatccgag ccagtgagcc
    28021 ggaaaggagt aaaaaatgga atcaacacct caatttgtcg gtagaagtct atatgactta
    28081 gatgactatt cttctgacga aattatgtat atcctggaag ctgccaaagg tatgaaggaa
    28141 ataaacctcc gggaatacaa aaaaatccct actctgcgag ggaaaactgt ctgtactcta
    28201 tttgtagaag atagcacccg cacccgaatg agttttgaac ttgctgccag tagattaagt
    28261 gcagatgtag taagtttcca ggctacagtt tcctctttaa aaaaaggaga aagcctgcag
    28321 gatacggtta atactctcaa cgctatggga atagacctct attgcatccg tcatagcagt
    28381 ccaggaagtc ctcaattagt gcataagtat tctggaaaac cagtaatcaa tgggggagat
    28441 ggaagacatg ctcatcccac ccaagcttta cttgatatct tttctatctg ggaacgtctg
    28501 ggtaatttaa aagggcttaa aatcactatt gtgggtgata tcttaaatag tagagtggtg
    28561 cgttctgatt taattggaat gactaaattg ggaatggaag taaccgtctg tggacctcgc
    28621 acgcttatgc ctgctaatat ggaacagatt tatggatgta aggtagaata tgatttagca
    28681 gaagccttga aagatgctga tattgttatg ggtctaagaa tgcagctgga aagaatgacg
    28741 gaaggtctgt ttcccagtct ggaagaatat agtcgtcatt atgttctttc caaagaaacg
    28801 attaaatatg ctaagaaaga tgctctaatt atgcatccag gtcctatgaa tcggggaata
    28861 gaaattttac cagagatagc tgatagcgct caaagtatca ttgtagaaca ggtagcaaac
    28921 ggtgtagcag ttagaatggc actgatgttt cttattcttg gtggcaagct ctaaaggagg
    28981 gggaaatgac tacactcata aaaaatggaa ttgcttatat caataatcgc ttttctcgca
    29041 aagacatttt gctcaaaaac ggaaagattc aaagaattgc tcaaaccata aatttggata
    29101 aagtagatca aattatagat acaagcggta aatatgtttt tcctggcttt gttgatattc
    29161 acactcattt aagagaccct ggacaaactt ataaagaaga tatcgcttct ggaactcgtg
    29221 cagctgctca tggaggtttt acgactcttt gtgctatgcc taataccgaa ccagtagtgg
    29281 ataatattgc ttctgtagat tatgttcagc gtagagcaaa agaccttggt tctgccagag
    29341 tttttgttat aggtgctatc actaaaaaaa gtgaaggact tgaaataagc gaaatggcta
    29401 ctatgaaatc aggtggaata gttgcggttt ccgatgatgg aaattgtgtc cagaatgcta
    29461 aattaatgct gaattgtatg cgttatgcca ccaattttgg acttcctctc atcattcatg
    29521 ccgaagatta taatttaagc ggaaagggac aaattaatgg tggaaaagta gctacaaaac
    29581 ttgggttaac tggaatttct cccctggcag aagaagttat tgtagctcgt gatattatgt
    29641 tagctgaaaa ctgcggtgca caattgcata tagcacatat tagcactgct cgttctatag
    29701 aattgattag agaagcaaaa gaaagaggag ttaaagtaac ctgtgaagtt acacctcatc
    29761 atcttttact aactgaagaa gcctgcctta attttgatac caacactaaa atgaaacctc
    29821 ctcttagaac cgaaaaagac cgtcaagcct gcgttaaagc tttaaaagaa ggaataatag
    29881 attgtattgc aactgaccat gcacctcatg cagattttga aaaagccaga gaatttgacc
    29941 aggcaccctt tggaataatt ggtttagaga ctgccttccc tgtgctttat cactatttag
    30001 ttaaaccagg ttatctatct ttggaaaggt tagttgaggc tttaacttca gcaccggtta
    30061 aaattctaaa tcttcctgaa agtgccttaa aaactggaaa tcctgctgat ttaactatca
    30121 ttgacctggg ggctgaaact atctttactc ctgaatctat cctttctaaa agtaaaaata
    30181 caccctggct taatcaaaca gtttccagta aggtattata tactttcctg gaaggaagac
    30241 aaacctggaa ggctgagtag tgcaatataa aatcttacct ttagaaaaaa tagaaaagat
    30301 tagaaccaac tacatccaga taactattaa ggatgaagat ttggcttctc aatgtaatcc
    30361 aggtaatttc ttccagataa aagctgatat aacttcacaa agctggcgtc tttttaaacc
    30421 tatcagtgtt tacgatatag aagaagaaag tatctccttt ctcattaagg tcataggtgg
    30481 aggaaccaga gctttagctg ctctaaaaac aggcgataaa ttgaagattt atggacctct
    30541 gggaaatgcc tttcctttaa ggcaagatag caaaatactt atggtaagtg gaggaacagg
    30601 ttatccacct ttagcttttt tgaaaaaaag gcttgattct tccaataata ttcttttttt
    30661 gcatggtgga gcttcaaaag atgatgtttt tccctgtgac tgttcttata ccatagatgg
    30721 aagctttgga aataagggaa tagtgacaaa agatatagct gatttgttga aaaaagaatc
    30781 ttttgattgt atatatagct gtggacctgt taatatgcta aaaactctgg ctcaaatagt
    30841 tgctcccatt ccacattatg tctctatgga agcatatatg gcttgtggag ttggcgtttg
    30901 ttatggttgt gttattccaa ccaatgaagg ttatctaaga gtctgttatg atggacctat
    30961 ctttgaagca gaaaaaatac tctggaaaga attatgagtg aactttccac ttctctgggc
    31021 aaaatcaatc ttcaaagtcc tattttaaat gcttcaggga cttttggaac tgaatacttt
    31081 aacttttttc agcagggaat tctgggtgct tatgtaacca aaacaattac cttgaaacca
    31141 aaaataggaa atccacctcc cagactttat gaaactgaag caggattact aaatgctatc
    31201 ggtctgcaga atccaggatt aaaatccttc ctcaataatg atttatttga tttgagagaa
    31261 tatcttaaaa tccctttgat agtgagcttt tcaggagcta ctcaagaaga gtttattaga
    31321 atgctggaag gactggaact acattgtgga atctatggct atgaaattaa tatctcctgt
    31381 cccaatgtgg aaaatgaagg aattgctttt gggaccaatc ctgatattgt ttttaatctg
    31441 gttgaaaaac tggcaaggtt aactgatagg gaattgattg taaagctcag ccctaatgtt
    31501 actgatattg ccttaattgc tcaagcggca gaagcaggag gagcaacttc catttcttta
    31561 atcaataccc tttatggaat ggcaataaat tgggaaacag gcaaatcaag attatccatt
    31621 ccagtatgtg gttacagtgg aatagggatt aaacctgtgg ctcttgcttt aacttatcaa
    31681 actgccagag cagtaaaaat tccagttatc gctatgggtg gaattcattc ctggcaggat
    31741 gctctggaat tcttttatgc cggtgcttct gctattgcta ttggcacggc taatttcagt
    31801 aatcctttaa ccattcctga agtttatcag ggacttctga attttttaac acaacgaaac
    31861 ctgaatttga aagatattat tggtaaagta aattgaaact tcagaagtat atataataag
    31921 caagcataat aataataact ccatataggg tgaaaatctt tttgaacttg acaaataacc
    31981 cgaatctttt acattatata ttgttatgtg ctcttttaca agtttatagg ggggtgatta
    32041 ggtttcgaca gggaatcagg gtgtgagtga tgcatgccgg agatgctgtc cactccgtta
    32101 ccaagcagca aacaaaatta actgcaaact acaattacgc attagcagca taggctgcta
    32161 cgtttaaccc gtccgaaccg acgggatggg attaagcgtc ggaacagttc ggttgcactg
    32221 gcacgaatgc ccataagtgc cagttaagat tatgggcttg gtcatagcag gtctgatcgt
    32281 cttgcactgc tatgacaaca cttaaagacg actaagcatg tagattcgct catagtcggt
    32341 tctttggacg cgggttcgat tcccgccacc tccaccaata aattgatata tctaaactaa
    32401 aagagaattt ctaaagcaaa aatagtaaat caatctgtgt aggagtaatc atatatttat
    32461 tattcatttt ataatcattg ctatttgaga ttatttcttc tacttattct ttccacatca
    32521 gaaaatccag ataatagata cagtttaaat ttactctata ttccttttat atcgccattc
    32581 tattatttaa ctctttttct atcttaaatg catagataat gtgtagctga tagggaggtt
    32641 atgtcccatt tcggacataa gctggacatc agttggacat aagctggaca tcagctcaag
    32701 ataaattgac gaaatagtag aaacttttta aaaggagaaa aaccagaaaa agcccaatcg
    32761 tactaaaaaa gctgaagctc tattcttttg cttatatagg agagttaata tagaatggga
    32821 aaaaaataaa atggagcggg caacgggact tgaacccgcg accctcagct tgggaagcta
    32881 atgctctacc aactgagcta tgcccgctaa taaaaaaaaa tttgattagg aaaattaact
    32941 gctaatctat aatgagaata taatgggata tcaccaataa ctatttgtat aattagggta
    33001 cattaactac attacctcct aaaatatctg catctataat gtatccagct tgcattgtaa
    33061 cattttctcc tttaataaat acgcctaata tacataaaaa taatgatact cctattgata
    33121 ttgcggttct tgattcaccc tttaaggtct tacttgctat tatggggagt ttagaaccat
    33181 caatagcatc aacggtagtt agagaaatag ctaaagatgc aggtttacct aacaatccat
    33241 tttgagtaac ttgttcaatt ctcccataag ctcttgctcc agctggtact attatttttc
    33301 catcctgata tacaggatgt actacaacaa actctactgg gtcaccttga tttgcagttt
    33361 ccgcatcaat agtttgagcc aacgataatt gaactcttgt tgggcgaacg acaacatttt
    33421 cagctacgat gggaagaaaa aggtttagaa aaacaatcgt aagcagaaat aaggcgatgt
    33481 acctacaatt tttaggcata agaacctcct atggtcttat tttattagag aaattttcca
    33541 tttctctttt attatatttg gaaacagctt gtaaacttcc atatatagat cccagataaa
    33601 atcctgttcc tattacagaa aagaaaacac ctgaaccggt atttcctcgt ttaaaattat
    33661 ccataatagt atagccaaat aaagaattaa ccactaaggt tgcaagtgca gtttgtggca
    33721 ttttacaata taaataccca gctcctggta agattgcttg aaaaaatact gcttttccac
    33781 ttttcttgaa ttttagaggt aaagattcat ccattattgc ttgaatgtca agagctcttg
    33841 attttggata gtccgtacta atttgattta atgtttcttc tgcttcgtta tattttccca
    33901 gatttattaa attaactgct ttataaatta gcaaagagtc ttgtttttct ttaggtaaag
    33961 cctgaataaa tttatcaatc aatttattag actcatagta atatttaatg tcagttaaaa
    34021 gaaatgtatg taattttatg gcgtcccagt ttatttcctg gtcttcaagc ttttcataag
    34081 tctttgccgc ttcaataaat ttatgggact tcgcataaca ttctgctatc ccaaataagg
    34141 ctctggtagt gtcttcttta cttccattat acatactacg ataatattca ccaatagcta
    34201 aatcatacaa tttttcactt tttaaaaagt cggcataggc aaaattatta gtaacttctc
    34261 catttaaaga gataaaaaac agtaaaataa ataaaatagc aaaaactctt aaattcatgg
    34321 tatatccagg aaatatattt catcatccac aattactttt atgtaatatt gtaaatcgtg
    34381 accacaacga tttaatctat caactgtgta aaatatgccc cttaatccat atttttctaa
    34441 tgcctctaat ccataattag aacaggaagg atacatagga caattttgtt gtttgaccgg
    34501 tgagatagct ttctgataaa attctaccat atattctatg atggttgtgg gattatctaa
    34561 ttttatgcct ttatttttct ctaaggtaga gggtatagaa atattagtta tattattttt
    34621 tgcagatata gcagataaag taattaaaat aaaaaacaat ataactccta tactttttct
    34681 catctaacta atctttatct ccattatgaa atatctattt aatattctat ttttgtattg
    34741 tcagcttaaa aacaaattca atattctcat gcaaataaaa aagtcaagta aattttaaga
    34801 ttttttagtt actttatata taacctagtt aatcaaaatc taagtattta ttcccattca
    34861 atagtagctg gtggttttga actgaggtca tagaccactc tattgatgcc tttaacctca
    34921 tttataatgc gattcgctac atacttcaaa aagttccaag ggaattcagt agcttctgct
    34981 gtcatcccat cctgagaatt cactgctctg agcgcacaga cattttcata ggttctttca
    35041 tctcccatca ctccaacact ttgaataggg aggaggatag caaaagcttg ccaggtctga
    35101 ttgtataggt tccaattatg caattcctga ataaagattt catctattaa ctgcaatttt
    35161 tgcacttttt ctttatcaac tgcacctata atccgaacag ctaaaccagg tcccggaaat
    35221 ggatgtctat ttatgagact ttcaggtaag cctaattctt ttcctacagc tctaacttca
    35281 tctttaaaga gttctcgtaa tggctctatc agttttagcc ccattttttc cggtaaaccg
    35341 ccaacattat ggtggctttt gatttttgcg gaaggaccac ggaaagaaac actttcaatg
    35401 acatcgggat aaagtgtccc ctgggctaaa aatcctacat tctcatattt tgccgcttct
    35461 ttctcaaata cttcaatgaa agtggaaccg ataattttcc gcttcatttc aggatcagaa
    35521 actccagcta accgagaaag aaaaagttct gaggcatcta caaagttaat cttaaagttt
    35581 tctatagcag aaaaagcttc ttgcacgcgc tctgcttctt tataacgcat acaaccatta
    35641 tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    35701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaaaatagg aagattttta
    35761 tgagctataa ctgcataagg tgcactggaa gttgaagcta aaacgataaa ctctggtgga
    35821 agttctgaaa tagtgtctcc gtgactcatc catacctttt cctccagagc taaaccttcc
    35881 agcaaaggag gtgatttttt tatctgaaga atagcctgac catattctct tttagtttga
    35941 tgctggactt tccctccaaa atgtgaaaca atgagttgca tcccataaca aatacctaaa
    36001 ataggaatat tcatttccca aatagaagaa tctggttgag gtgcatcttc ttgatttata
    36061 ctgaaaggac tgccagaaag aattattgcc ttaggattga tttgttttat ccttgtttcc
    36121 gtcaaatgat aaggatgaat ctcacaatag acattgaaag aacgtatcac ccttcctatg
    36181 agttgagtat attgggaacc aaaatccaga attaaaacgg tatcgtaatg catcaagtta
    36241 actcacaaaa ggattgttct tcttttccat acctattgaa gtgcgaggtc catgtcctgg
    36301 aaaaactatc acatcatcag gtaagacgaa aagtttattt tttatagaat tgataatctg
    36361 ctgatgattt ccaccttgca aatcagttct tcctatactc tgttcaaaaa gggtatctcc
    36421 gcttattaaa aatttatctg ccagaagaca aataccacca ggagtatgtc caggagtatg
    36481 aatcactttc acaggatact ttcccagact caattccata ccatcttcta aaagcacatc
    36541 aggtggagca ctggatacag gatttcccac atataaactt aaattcattg cgctattaac
    36601 cagcattgga gcatcagcag aatgaatcat aagtggagca gaaagatgat ttttgaacca
    36661 gctatttcca cctatatggt ccatatgccc atgcgtgtta attatatatt tgacctttaa
    36721 cttcaactct tctatccttt ttaataaatc aggagaagga gcagccggat ctaccaatat
    36781 agcttctcga gactcttcat cccataaaag ccaggtatta gtttcaaaat caggtaatag
    36841 aatataacct tcaatcttta acatcaggaa tcctttatga ggtaattaga tatgacctga
    36901 ggatattttc aggacatttt tcaactttta tctcttgatt gaactttttg tcaactccat
    36961 atttaatata tgcgggcgaa cttgctttat gactgtaaaa aagaacaata gaagcagcga
    37021 gcttaagaac ctcttcagaa ggctcttgaa ctgttagtaa ccccaaaggt ccttctacat
    37081 ctctaatctt tagatataaa ccaggatagt tctcactttc aattcctgca ttatcagcag
    37141 catctcttcc tacaatcaat ttaatctcat ctgttaatct aaaatgccta ccccagcgta
    37201 gaagttttac attaggcagg atatcctgat tatgtatcat caaatctctt aatcgcagag
    37261 aataattatg atcggtaaga agacagcctc ctgcaggttg aagatgagat attccaaatt
    37321 gctctgcaag ctgtagctga cgcgaacgtc ctcttcctga aatatccagc atttgctctt
    37381 tatttaccca tccttctctt atgggctttg tatcaggaag taatttttga gataaaggac
    37441 ggataactaa atctcctgct ccactcattt tatctactgc tttaagagaa tccagatttt
    37501 gggacatagg tcgttgtccc agcacttctc cagtaataat aaaatctgct cccaattccg
    37561 gtaacatcaa tgctgcttct tgaatcatca acgaatgaca atctatacaa ggattaagat
    37621 ttttcccata acctcttttg ggatgacgca acatctctaa atgcttctcc gatatatctc
    37681 taacttccag tttaagtcca ttttcttctg ccacttttaa tgctttatcg ggaaatatat
    37741 aagcagctga aaaaaagact ggaataacct caaaaccgag cttttgcatc caaaaaactg
    37801 ctaaaacact atccagtccc ccagaaaaca gtgctatagc acgctgcggc ataaattcta
    37861 ctttatttgc tattgatatc ctcctgaatc aaatccataa attttaaagg agagcgatga
    37921 gcttgccatt cattttgacc cttaccattt gcattccacc agtaataatt attactaact
    37981 cccacaatta aaactttgtt atcgtgtttt ctctgatagc caatagcatt gatcccaaag
    38041 tagaaattct caatcggagt ttgattttgc cattccgttt tcaccagggt ataagtatta
    38101 atattaccat caatttgacg aatgtattca caatgctgac tacgaccctc ccatccagca
    38161 tctatcacat taaaatcagg attttctgca atagaccaga gtggagaata attatagtct
    38221 tgagaaaaag agggccaggg atcaaccact atatttttag gcagaaagtt tatactgcca
    38281 gtataggttt tgttattagc taagtttgtt attttgtatg aaataggctg atccgtgctt
    38341 aaatttatag tcgcacctct ataaatttta ccatctgtgt aggactctat attattaaat
    38401 acaatatcag ttggtccaat tttcaacgaa gcatctgaag catttccttt agcagtcatc
    38461 catatatctg ctgcaaccat atttttgctt tgagcagtat aatccaaatc aaaccagacc
    38521 acataattat aatcccatat atgtgaggat ataccatatt cttcaaaatt gggagtggta
    38581 ggagtttctt cttttgaaca tgataatcct cctaaaatca aaaaggttaa aagtaacatt
    38641 gacggaatat aaagataatg tctcattttt tgatcctcct ttaacattaa caactttata
    38701 aagaaagagt atttgtattt taaagaataa caaatgatat ttatgaatta ttaggataaa
    38761 ttagacataa aaatttgtat tcttctggag ctaaatgggg gacttgaacc cccgacctgc
    38821 tgattacgaa tcagctgctc taccaactga gctaatttag cttaaagaag atataccttt
    38881 tatgtttata taaaggtatc ataattcata aaagcgtttt ccgtcaagaa ggatttgaag
    38941 attcatatta aggtttattc ttccacaaga aaaaaattat agttaataaa ggaaataggt
    39001 attccctgaa caattaagga agcaagattg attttgctga agaggagaat ttataagaag
    39061 aaaaaggata ggcaagaagt gaagaaccag aagaagatat ttattcgttt tctggtggat
    39121 ataaagtatc ttacggatat aatagaatac gaaatctgga gatggagaac actaacttcc
    39181 cgaatatctt atcaaaacca gagattataa aactttagct acttatcttg cctatagcca
    39241 taagaaatca tctactgcta cgactatctt tattgactat tggcttgtat cttaatacca
    39301 cccattgtgg ttgaaaatta tatccctctt acgcaaaaaa aatcaaagat agatactttt
    39361 cgcatttacc aacttcccga aatgcatttt accttgacaa catagagcta aataaataat
    39421 aaatttcttt ataaaaataa agttataata aaataaataa taaagattaa aagaagaaag
    39481 gaggtaaaaa aggaagatga gtatgtttcc cctgagtaag caagtgagct tagtcaatct
    39541 tcttaagatg tcaaaaactt taaaggcaaa cgcgaggttt ttattatgaa aagaacattt
    39601 cttatcgttt tattattact cagctgcgtt tttatctttg ctcaattaag tccttggctc
    39661 tgggcacaga aagctggtgg gacaggtgaa gattctggcc acagtattgc ggtagatgct
    39721 agtggaaata gctatgttac cggtggtttt gaaggtactg ctatcttcgg ttctactact
    39781 ttagaaagta gtggatctca agacattttt gtcaccaagc tggacaaccg tggaaactgg
    39841 ctctgggcaa agaaagctgg aggaacagat tccgattatg gctatggtat agcggtagat
    39901 gccagtggaa atagctatgt tatcggttgt ttttacagtg ctactgctac attcggttct
    39961 actactttaa caggtaacgg atataacaac atttttgtcg ccaagctgga cagcagtggc
    40021 aactggctct gggcaaagaa tgctaaagaa agtccttacg attatggcca tggcattgat
    40081 gtagatgcca gcggaaatag ttatgttact ggttattttt ataatagtgt caccttcggt
    40141 tctactactt taaccagtag cggaggtgcc gatatttttg tcgccaagct ggacagcagt
    40201 ggtaactggc tctgggcaaa caatgctgga ggtacaagtg acgaatgtgg caatggcatt
    40261 gcggtagatg acagtggaaa tagctatgtt actggttatt tttacgatag tgccactttc
    40321 agttctacta ctttaacaaa taacggagtt tacgacattt ttatc
//
LOCUS       DAORZF010000010        36836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_917_length_36836_cov_367.126234, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000010 DAORZF010000000
VERSION     DAORZF010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 36836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36836
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_917_length_36836_cov_367.126234"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..1099)
                     /locus_tag="PK015_02405"
     CDS             complement(104..1099)
                     /locus_tag="PK015_02405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOL33358.1"
                     /translation="MRILVIRLSSIGDIILTQSVVAELRELFPDAEIYFLCKPEYQAL
                     PELFGLNLKIIPYSKSFSWHINLTKQSYDLVIDLHNKFSTWLIRNLVRSHKKAIYNKQ
                     HKLRKRIVRGNHSISIESTVRLYYSALEKLFPDKFSSTYSIRYPVLHPELIPEEIEIT
                     PRIYPDRKLIALFPGAAHNTKIWQIDNWKKLIEDCHNEYEFRIYGNQSDYELTRSLNA
                     VFDDVKNLCGKLNLRQLTAEINACDAIISGDTGPMHLAAALRKPQIAIFGGTHPRLGF
                     APLNDKAIVLTADLACQPCSLHGRNKCPKGTFECMKQITPEMVEKALENIFIEDN"
     gene            complement(1099..3699)
                     /gene="alaS"
                     /locus_tag="PK015_02410"
     CDS             complement(1099..3699)
                     /gene="alaS"
                     /locus_tag="PK015_02410"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOL33359.1"
                     /translation="MWTSKEIRQTFIDFFRERGHTFIPSSPVIPWDDPTLLFANAGMN
                     QFKNIFLGLKEPEVKRAVNSQKCIRAGGKHNDLEAVGKDGYHHTFFEMLGNWSFGDYY
                     KKEAIEWAWELLTNVFHLPKDKLFATVHNSDEESYNLWKNNTDIDSSHISYFGDKDNF
                     WEMGETGPCGPCTEIHYDRGIEHCMKKNVPGHKCELNGDCNRYIELWNLVFIQYNREE
                     DGSLSPLKSRYVDTGAGFERLVQILQNTYSNYETDLFMPIIDKIVQMSGVPYNPETGM
                     SHRVIADHIRCLCFALADGGFPSNEGRGYVLRRILRRAARHGRLLGFSEPFMFHLVDS
                     VVEIMSEPFAELKGKEAYIKMVIKAEEERFNSTLDKGLERFDQICANSAEKIISGKDA
                     FQLYDTYGFPLDLTINLAEERGFKVDTAGFEEEMKKQRERARKSSKFIYKWEDMDWIE
                     LMPSTPTDFVGYDNFSIEAHIQRYRIDDEGNLHIQLDKTPFYAESGGQVADTGRIYND
                     DFEMQVIDVKRIDDKFIHIGHLVRGAISSPLVKAEIDLQRRKDIARNHTATHLLHKAL
                     RMVLGEYVQQKGSFVGPDYLRFDFTQLQALTQDEINKVEDIVNEIVKENRPVHIEIKD
                     IDEARSEGAMALFGEKYGQKVRVISVEDFSKELCGGTHISATGEIGLFKILSESSSSA
                     GIRRIEAVTGRGAEKFVRSLQERIANLAAVLNVSEKMLETKLETMQNLIKELEEELKK
                     KEQRQSFADVDKLLDKIVEYPDFSLLCTTVETEPERLRELGDLLKDRAKDTISLLFTI
                     EGDKITILCVVGRDIMDKFNAGKIVKAVSQELNGKGGGRADSAMGGGKNPEKLSELMP
                     RIPDIIRSTL"
     gene            complement(3700..4644)
                     /gene="trxB"
                     /locus_tag="PK015_02415"
     CDS             complement(3700..4644)
                     /gene="trxB"
                     /locus_tag="PK015_02415"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOL33360.1"
                     /translation="MQYDVIIIGSGPAGLAAGIYAARGGLNTIILERAIIGGQITSAH
                     QVENYPGFPDAIEGFELTERMRQQAERFQAKFLDEEVTAIGMEGLCKIVETREHKYRT
                     KALILCTGAYPRRLNVPGEQRFTGRGVSYCATCDGALYRDKVVAVIGGGDSAVEEGIY
                     LTHFARKVYIIHRRDELRAQKILQERAFQNPKIEFIWNSVVQEIHGSDKIEKLEIFNR
                     VTQEISFLPVDGVFIYVGILPNNKLLESRINLDEAGFVYTDEFMHTNIPGVYAAGDIR
                     HKVLRQVVTATSDGAIAAFSAEKWIRDNYEALEKTEQN"
     gene            5249..5383
                     /locus_tag="PK015_02420"
     CDS             5249..5383
                     /locus_tag="PK015_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33361.1"
                     /translation="MKVITVKIKIHPQYPMFQLDFNSSLILNLNYNDIILRNYVSYGL
                     "
     gene            5373..6170
                     /locus_tag="PK015_02425"
     CDS             5373..6170
                     /locus_tag="PK015_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOL33362.1"
                     /translation="MDYRVSVVQFKPEFLAITENLNKLKAILSEMETDLVVLPELCLS
                     GYVFQKQEEVEKVSETIPEGFVFQEFRQLSTEKEFSIVYGFAEKAENRYYNSAALINP
                     DGSYYIYRKTHLFYREKLFFTPGDTGFQVFPAKGNILIGIMICFDWQFPESARTLALK
                     GAQIICHPSNLVLPWCQQAMKTRSLENRVFSITSNRVGIEYNNGETMEFTGKSQILGT
                     KGEVLLQLDEKNEGIGTIIIDPNLALDKSVNPLNDVFKDRRREMYEL"
     gene            6167..8155
                     /gene="ligA"
                     /locus_tag="PK015_02430"
     CDS             6167..8155
                     /gene="ligA"
                     /locus_tag="PK015_02430"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOL33363.1"
                     /translation="MNEELKKEIQKLRAEIEHHNELYYKLAKPEISDYEYDQLVLRLR
                     QLEAQLSAEELKESPLEKIGSDLTPGAETIPHKQRMYSLDNTYSANELKDWIYKLATD
                     LGFFPTLCAELKIDGFSINLYYENGVMQYATTRGDGIVGEVVTNNVKLLKDIPHKINF
                     TYPIEIRGEIYMPVEEFLKLNEQRRANEEKPFANPRNAAAGSIKLKDPEEVKNRNLRA
                     IFYSVGYCENLPVQRQSDFLNWLDSLGFPTAKQRCVCNSYDEVQKFCDEWENKRYTLP
                     FEIDGIVVKVDDFALQRKLGYTAKSPKWAVAYKFKPEMKETRLIEVQYQVGRTGAVTP
                     VAILEPVYISGSTVSRATLHNEDEIRRLDLHEGDTVLLIKSGEIIPKILQVIPEKRKP
                     NAKPVSFPTQCPACGSPLQKDEEGAIHYCSNVACPAQLQRRIEHFASRDAMDITGLGE
                     SLIARLLENKLISGIADLYRLDYDKLAQLEHFGPKSAQNLKNAIEASKTRNFDRVLYA
                     LGIRYVGTVTARYLAEYFGDIDSLRHASLESLSQVPEVGEKVALAIKAWFSLPENIKL
                     IEELRSLGINFTYRRTQASNALAGKTFLITGTLPHYSRREMEDLIQSHGGRIISGVSS
                     NLDYLIVGENPGSKLTKAQKIPTIKIITEDELLEMIKQ"
     gene            8152..9480
                     /locus_tag="PK015_02435"
     CDS             8152..9480
                     /locus_tag="PK015_02435"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOL33364.1"
                     /translation="MKILSRYILKEHLSPFFLSLLVVTFVLLIDRAIDLMNTIIEKRL
                     DIGTVLQLFGLSLPYMLSLSIPMAVLVATILTFGKLTVDRETIAMKASGINIYSLLGP
                     LFIAAILFTGLMVYFNHYFLPNTNHKLKNLTVKIAYYRPMTIIKPKEFTTISDYTIYT
                     EENLNDELKNVLVYDRTQANFPRIITAQKGRIVQMDKGNSLQVILQNGEMHNRNEQEK
                     GKYQVTKFETFTVNIRDLAVGMDFGESGYRSDREMTYNQLVADINNKKQELTNQKLEI
                     TNLQKRLEELQKQPDDYAKSVETRRLSIMKSMAEDQYKEILENLRSLQVEYHKKFALA
                     FAIIIFVMMGVPIGLMTRSSGIGMAFSVSSVIFLIYYIALTGGEQLADRALMSPFLSM
                     WITNIIFFIIGLWLIHLSIHEKQLINLNLLQWKLSHRKAKPEETPDEIIH"
     gene            9490..10578
                     /gene="lptG"
                     /locus_tag="PK015_02440"
     CDS             9490..10578
                     /gene="lptG"
                     /locus_tag="PK015_02440"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HOL33365.1"
                     /translation="MRILDRYIVREFLKTYLVIFVCFAAVFTVIDVIDNLPKLIRNGA
                     TVEQAIEYYLLRLPYLLVLTSPVTILITGLFLMDSLSKHNESVAIRAAGISIHRTMLP
                     LFLVGLIISIIVALFGEYVVPWAEKTRNYVYNVEIKGEPEESNMLKARVHYRGTKNDF
                     YYFGFFDGYNNTLRVIDLAQLNPESKEIAKHITASAAVWQNDKWIIQDCEIRDFGQGQ
                     QTQYQYYPSTDLPLLDVQPKDFIRITKKTLSLNFFELKDYIARLQKMGEKAHKEIVDL
                     HMKISFPLTNLIVIFFCIPIATTNIRSKGRGLIFLLGLVVCFIYLIFVRVFQSLGYNG
                     VIPPVLAAWFPNIFFTILGLFFLHKAEI"
     gene            10654..12441
                     /locus_tag="PK015_02445"
     CDS             10654..12441
                     /locus_tag="PK015_02445"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOL33366.1"
                     /translation="MLDQLVKKQKVIIYIIAIAFILSIGAGGIFGGEKIIQALGGNYL
                     GKVNGTKITPQQYNLKIQEIKERYEAQGQKIDESYMPYIQNTAWEELVNEILWQQQIK
                     KHRIKVTEAEIKNAIENDIPQELLQNESLQTNGVFDKKKYFQALNNNPQFKLQMYEYM
                     KTYLPRKKLQQKIMSEAGITLDSLKQEFIKENDKVYGKTIWFSYHKADSVYVSDAEIK
                     KYYEEHKDTEFKKGPASRIKYITIPIQPSERDYNEVKLEIDEIYRMVNPENFPIIAEQ
                     YSEDESSAKQGGSLGVFGKGQMVPEFEKVAFSLKPGEISKPFKTDFGWHIVKCDSIIN
                     DQIKASHILLKVNTSDETKRELYEKAKQARKLIAKKGIDAAAKELNLEVITSDWLPHD
                     QQQIPGIGQHGGLHQFMVKKKPGKVSEVYQLNIQGKPSYLIAQVVENRKFYYEDFNAK
                     KLQIKYDLERQKKIALMKAKAEDFIKRVPKDKYFDVAAAEGWDIIELKGHKKDSRLPA
                     PVNATLEEFDKAALALNTGQYSDIVNTKEGPFIIYAEKREKPDMQQFTKEKQEEIRKR
                     LEEAAFNRWWQQIRKEAKIIDNRYKYGYY"
     gene            12448..13821
                     /gene="glmM"
                     /locus_tag="PK015_02450"
     CDS             12448..13821
                     /gene="glmM"
                     /locus_tag="PK015_02450"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOL33367.1"
                     /translation="MSKLMTSVSGVRGIFGETLNPIVALQYASHFGQFIAQESANPAS
                     HHIPKIVVGRDSRTTGKAMLYAILSGLISTGCEVIDLGIVPTPTVLLNVKKHSAQGGI
                     SITASHNPPQWNAMKFVDGDGMFLSPEKASRFIESVNAEISWNDWQNMGTVTEDAEAI
                     NYHIQRILAIPYLNIAQIRSKKFKVVLDSVNGAGGLASPLLLKALGCEVVEINSEPTG
                     VFAHTPEPLNENLHQLEENVKLHNADLGFATDPDVDRLAIVDETGHCIGEEYSVALAE
                     LFILPKKPGDIVVNLSSSMLSDYIAHKFGVKVHRTRVGEIYVGKKMKELHSPIGGEGN
                     GGIICPEVNYTRDALTGMALILALLAETNQKLSEIVTSLPRYYVAKAKIELPIEQIDK
                     ALKKIPSLLPDYELDFQDGIKAIAPDHWIHIRKSGTEPVIRIYAESDSLQRSEQLCQD
                     ILNKLNS"
     gene            13978..15174
                     /locus_tag="PK015_02455"
     CDS             13978..15174
                     /locus_tag="PK015_02455"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HOL33368.1"
                     /translation="MRKTVVVSAKRTAIGNFGAAFKDVSAVDLGVAVLKAMLSETGIK
                     GEQIDEVIIGNVCGAGLGQNVARQISIKSGIPVTVPAYTVNKVCGSGMKSVTLGALMI
                     GTGEADIIVAGGTENMSQIPYASPDTRWGCKMGNRNLVDLMIRDGLSDIFNDCHMGLT
                     AENLAEKYHITREEQDIFAAQSQNKAEAAIKSGRFKDEIVPIIIPQRKGDPLVIDTDE
                     MPRPGVTPESLSKLRPAFKDGGTVTAGNASGINDGAALLLLMSEEKAKELGLPIMATL
                     VNYASAGVDPAYMGYGPVPAIGKVLKKSGWNFNDLQLIELNEAFAAQSLAVFKGLEEE
                     GIGKIDLSITNVNGGAIALGHPIGASGARIIVSLLYEMQKRDVHKGLAALCIGGGMGI
                     ASLWER"
     gene            15187..16431
                     /locus_tag="PK015_02460"
     CDS             15187..16431
                     /locus_tag="PK015_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOL33369.1"
                     /translation="MADKLKLNKSMALFEESQKVVPGGVAGIRRPYNFVPGEYPIFFE
                     HGKGGHITDVDGNEYIDYLCAYGPIILGYREEEVDNAVIEQIRNKGICFSLTQPVQTQ
                     LAQKLTEIIPCCEMAAFVKTGSDATTIAIRVARAYTNKVKIARCGYHGWHDWCVEVKT
                     GIPEKLYEDILEFHYNDLDNLEDILKANANDMAAIIITPVGHPLGNPVEMPKPGYLEG
                     VRQLADKYGCVLIFDEIRSGFRCSLGGAQEYFGVTPDLATIGKAMANGYAIAALVGKR
                     EIMDVLTKDVFLSSTFFPNSDAQVAALKTIEILERDKVLEVIAEKGKKFGAEVQKLVE
                     ESALPIQFTGAPWMPYITFNRDPEGLYKKLRNEFYTQLIRRRVFLQPYHHGYICYRHT
                     DEDLAYTVQTIKESLDELKKLV"
     gene            16532..18148
                     /locus_tag="PK015_02465"
     CDS             16532..18148
                     /locus_tag="PK015_02465"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOL33370.1"
                     /translation="MAKYIFVVGGVLSSLGKGIATASIGLLLKSMGYKVVLQKFDPYL
                     NVDPGTMSPFQHGEIFVTDDGAETDLDLGHYERFTDVSLTRHSSSSAGQVYETVIQNE
                     RRGVYQGKTVQVIPHITDEIKHRIRALEADYDIIITEIGGTVGDIESLPFLEAVRQLR
                     LDLGYKNTLYIMLTYVPYIKSAGELKTKPSQHSAYKLREIGIQPEILLCRSEKPFGKE
                     IYDKIALFTNVPRENVINAIDVKSVYECPITFKNANLHKLICQHFEIECRPLNLRSWN
                     KFLTNSQDCVDKVTIAICGKYVRHQDAYKSVEEALVHSAAWHRLKLQIKWIDSEQNFK
                     SALLNRSLQDVDGFLIPGGFGVRGIEGKIAIAEYARTHKIPYFGICLGMQVAVIEFAR
                     HICHLKDANSTEFDPKTPHPVIHLMEEQQYVEMVGGTMRLGAYSCKLKRGTLARKAYG
                     KVLISERHRHRYEFNNAYKEKLESKGLTFSGFSPDGLLVEIIELPAHPFYIGVQFHPE
                     FKSRPNKPHPLFREFISAAYQYHQSHSTPV"
     gene            18315..19331
                     /locus_tag="PK015_02470"
     CDS             18315..19331
                     /locus_tag="PK015_02470"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOL33371.1"
                     /translation="MRSSITLLIGIALIFAGFCLSCEKKTSPEETITSQEEKSVPPTH
                     LHKVRYTIKWLHQAQFAGAYMAKEMGFFQQYGLDVEIIQGGVDNPPYKALINGKTEFC
                     SMNLITAVERNNPEFPLINLAQISQKNSSWLVGKKTTGIKNLQDLNGRKVGVWRDEAG
                     DHIRYFLNDNKIRAQIIPLDWSVNLFLNDAIDMMNVMSYNEYHRILMAGMEPEDLVVF
                     NLSDYGYDLVDDGTYTSRAYYIQHPELCKNFTKALIEGWIYALNHPDETVNVVVRYMR
                     DNHLPANYNHQYWMLNRMRERILENPDKVGYLNPEDLALAEEILKRNGKLEYPVEYKE
                     FFLQ"
     gene            19328..21232
                     /locus_tag="PK015_02475"
     CDS             19328..21232
                     /locus_tag="PK015_02475"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOL33372.1"
                     /translation="MKTETKPRTIAFQIISFMFVVLVALFIISILTTGRLMHSVMESN
                     ARESFGNITKINVQTIDQTLSKMVILGRDLELLIKDNNHETDLNLHIRQLLTDNPEIV
                     SVCIAYANKSHTFSNTYLLQGANFLINPSISDDFSYKDWFLLPKLTEKPYWSDPWFDS
                     DGTGTIVCSYSIPLFIKGNVEGVLRLDTSMENLRQIVSSVRVKKTGYAFLISNNGTIV
                     AHPQDSLAMNYTIFDLAEQLNSPKIRSIGKSIVNRKSDFVHLNKIGIPANVWMFYQPV
                     PTNSWSLATVAPDREVFADLYNLMFIYIGWVLLAFLIIAIAIWYRTRTINQPLERLIQ
                     SIEKSQSGVPATATLPDSDTYEIQVLNTNFNRLKNSLKKYAESLQEVTAEKDQIMDEV
                     TFASEVQLNLIPNNQKSFTLPPNISAYGILEPAGLVGGDLYDYFSVDDEHFLFGIADV
                     VGKGVAAALTMTMVSTLLRTYAPQLKQPEKILTYMNKFLIENNPDYRFVTMLLGIIDL
                     KTGTCTFSNAGHTPLFLLSEDGKITKYAQTHSTALGILENLNASSETIQLKSGDEIVL
                     CTDGITEAVNQEDKLFGTDRLEKVLKNLKNPEPESTAQAILDAVKSFADPERQTDDIT
                     ILVVKYMNVS"
     gene            21277..21927
                     /locus_tag="PK015_02480"
     CDS             21277..21927
                     /locus_tag="PK015_02480"
                     /inference="COORDINATES: protein motif:HMM:NF025015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HOL33373.1"
                     /translation="MKLRKSEIISLVIVIISLIIGIYFYPLMPEKVASHWNTEGEVDG
                     YMPKFWGLFLMPIISAVFLFLFIIIPKIDPLKHNIEKFRKYFDGFIVLMMVFLFYLYI
                     LTILWNMEIRFVFTQFLVPIFSILFYYCGILIQNAKRNWFIGIRTPWTLSNEEVWNKT
                     HKIGGKLFKIAAIITLIGVFSPKYAFFFCIIPVILASLFPIFYSYFEYQKLSKNLK"
     assembly_gap    22223..22322
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            22515..23042
                     /locus_tag="PK015_02485"
     CDS             22515..23042
                     /locus_tag="PK015_02485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HOL33374.1"
                     /translation="MKQQKVKLIAVVFAVWSIFCGPIIAQSITEITAKEAVEARTSQD
                     ALIDSLLTKEPPDGITAPVIQETPPGEIWLASYYSKRFNGKKTASGECYNQYAYTCAH
                     KTLPFNTLLQITNPKNGKTVNVRVNDRGPFVKGRDLDLSYAAAKDLGLLNHGVMKVVV
                     SILPETNENINLALE"
     gene            23131..24057
                     /locus_tag="PK015_02490"
     CDS             23131..24057
                     /locus_tag="PK015_02490"
                     /inference="COORDINATES: protein motif:HMM:NF016142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HOL33375.1"
                     /translation="MLPASIKIAPSVREALTEDKPIVALESTVLTHGLPYPENISILK
                     TLENILLSQDVTPATIIVLDGIIYIGLEENIQNKLEQMLKNRENFSKLGMRDLPLAIT
                     QKRSGGTTVSSTMLLAHLSGIKVFATGGIGGVHRNWENSLDISSDVIALSRIPVIVVS
                     AGCKAILDIPATLEVLETLAIPVLGWRTNSFPLFYTATSDYPIERLNDTEQFAAFWKN
                     HLAFQGKGVLIANPIPEEYSLPPEILDSVINKSMEQAHIKGIKGKELTPFLLDFIAQA
                     TKGESVKANLALLKNNVQLAAELAKSLINKGS"
     gene            24061..24897
                     /locus_tag="PK015_02495"
     CDS             24061..24897
                     /locus_tag="PK015_02495"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HOL33376.1"
                     /translation="MRIYISIDMEGIPGTFNWEQEKTNRATIQNCMFHHIQTVIESIL
                     ASPQASLIEEITLADSHSAGDNLDYSITALDERINLISGCPRPRYMMPDLDPAYDQVW
                     FLGYHSGTGALRGNMDHTYSNSRIHNIWINGERMNEALINAAYAGYSGVPVGLVSGDE
                     TLKDELLLYLPWIEYIPTKKAIAKFSAKNYSQKLVDKQTKSSVLQTLTRTKEEIPLFT
                     FEPPLSLKIEFNHSSMADQAQLMPYTKRLDGRTIEYQTDDYAILFEAIMVLVYLAQIT
                     PM"
     gene            24913..25704
                     /gene="pyrE"
                     /locus_tag="PK015_02500"
     CDS             24913..25704
                     /gene="pyrE"
                     /locus_tag="PK015_02500"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOL33377.1"
                     /translation="MEDNLVTIAKFEDNFEADLAQRLLADNGIQAFVKNAIFYSMMPG
                     VFPGKVDLELQVNSEDEEKARAILSEYQNTPEIKRILVRNDVLQEGHFLLTSGRHSAN
                     YLEKIHLYQNPQDTARVCEMLAELLVDYDFDTVVGPAYGGIVLAFETARMLEKGFLFT
                     QRKEGVMTIRSGFDLSKVKKAVITEDIITTGGSVKEVITTLKNAGIEVVAVAAIVDRS
                     TEEMDFGCPFLPLLKMDIPSWEADDCVLCQNGIPLTVPGSSDKKI"
     gene            25711..27186
                     /locus_tag="PK015_02505"
     CDS             25711..27186
                     /locus_tag="PK015_02505"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOL33378.1"
                     /translation="MPELAIITLNRREHSVLSHLWKFFPFESDIQYHYLYPGKEDEEI
                     TSLRSNVHSLAFSGKLEEVIEAMDNYLKENNLSHICFSSLETQAVWSRYYKQNAVTIY
                     TSQDLYLADISVLPSACPEKLEAISDLVKRDIAVLLSEAGTFLLPENTTIYPDFNSFT
                     RTMSHEILLTKNKDLVSAVCKASVRHYFVLHLINNKVSLNLSLDTESFDFGYVFTNLS
                     LATKWIKEGIDSALQERNKGYLPYSVFAYYYDIYMSHVNYDEWIDGIIMWYKQMNPNP
                     LHKILELACGTANASSLLAARGFEVDACDASPYMLQVADSKPIKPNLYQAYLTDPIPK
                     KDYDLIFCIFDSINYLLHPEEISTVLDNVYEALAPQGVFIFDISTLLNSMVNFYDSTN
                     YLKVHDAYIIQRSNYNTFANHQLTHIIIFRKHFHNYELSEERHTQRVYYTPELIKLCN
                     ASKLKLKAIYSPEKNHNLLGKNVEKLDKNFFRLFFVLQKER"
     gene            27183..27803
                     /locus_tag="PK015_02510"
     CDS             27183..27803
                     /locus_tag="PK015_02510"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOL33379.1"
                     /translation="MNPLYEFDSVFLKNSERIIGVDEAGRGSLAGPVVIAAVILDLKN
                     PIEGINDSKKLTPIKREKLFEQIIQSAIAYKIVEVDVNYIDKYNILQATLKGILESAS
                     AISQPFDLCLIDGNKVPDGLFCPSESIIHGDALSASIAAASILAKVYRDRLMRNLDKY
                     YPLYGFAKHKGYGTAQHLTALKLYGPCSIHRKTFSPVSEMMESQIK"
     gene            28074..29681
                     /locus_tag="PK015_02515"
     CDS             28074..29681
                     /locus_tag="PK015_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOL33380.1"
                     /translation="MEKMMLLGDEAVAQGALDAGISGFYGYPGTPSTEIIEYAQQSKQ
                     AEEMKVHRNWSSNEKTALEAALGMSFSGKRAMVTMKHVGLNVAADPFMNSACTGAHGG
                     LIVAVADDPSMHSSQNEQDSRFYGKFAMIPVLEPSNQQECYDMAFFGFELSEKYHIPV
                     LLRLTTRLAHSRSGVIRRNSIPQKELKLPEDLYQFLLLPAIARRRYKHLIELQSDFLK
                     EAEESPFNQLTLDAKDYSVGIITTGLAYNYLRENFIDQPIPYPVLKISHYPLPEKAIH
                     QLYDKCDSLLVLEEGMPFAEDILKGLSWTGTKPIHGRLDGTIERDGELNPNKVARALG
                     LPDTEGKPVPSVVRPRPPALCVGCPHSDSFLALNEALKDYGKGHVFSDIGCYTLGFMP
                     PYNAINSCVDMGASITMAKGAADAGLYPSIGVIGDSTFFHSGLTGLMDCVYENANVVI
                     VILDNSTTAMTGGQEYTGYGKIEDVCKGIGVHPDHIRIFKPLKTNWDEMVKIYQEEIA
                     YNGVSVVIPRRECIQTLRKKNKMETPE"
     gene            29678..30244
                     /locus_tag="PK015_02520"
     CDS             29678..30244
                     /locus_tag="PK015_02520"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOL33381.1"
                     /translation="MKKDIILAGVGGQGILTIATVLGAAALNRGWNLKQAEVHGMSQR
                     GGDVQSHLRLSDQIIWSDLIPYGKADMILAVEPMEALRYLPYLAPEGWIISNKTPFKN
                     IPDYPDEEELYAEIRKIPNNVLIDADSIAKSVKSPRSMNIVMLGAAIKYLEFTPEELK
                     ESIKMIFNRKGERIVEENLRALDAGINA"
     gene            30302..30757
                     /locus_tag="PK015_02525"
     CDS             30302..30757
                     /locus_tag="PK015_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33382.1"
                     /translation="MKKRKGLSNRLFRFWFFFLSLNISSLPAEIRFQTIEDFESGYVN
                     LLSYSNEDQNPTAWELTSSDTYNNSAWALRLYGNTWKLQMINPVVVDSGAVFQVAAKT
                     STGAKIQGVGFSDGNNVLFYSFAGTQTLNIEEWVTVYQGALNVLEWKRF"
     gene            30893..32260
                     /gene="xseA"
                     /locus_tag="PK015_02530"
     CDS             30893..32260
                     /gene="xseA"
                     /locus_tag="PK015_02530"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOL33383.1"
                     /translation="MDELIVFSVSEVTSHLKQVIESQIEPLYVRGEISNYTRHPSGHI
                     YFSLKDANATLRCTFFRGSNIYLDFEPEEGMEVICYGKLTIYEKGSTYNLNVYSMQLA
                     GKGDLARRFELLKKKLQAEGLFEEAHKKPLPKFPRRIGIVTSPAGAALQDILNILRRR
                     YPVEVIVFPALVQGEEAPPTLIQGIRYFNEKENVDVIILARGGGSQEELFCFNDEQLA
                     RAIYDSKIPIISAVGHEIDFTIADFVADLRAPTPSAAAELVVPNRVDIINSLKALEEK
                     LTNSIKNKIYITRNQINELRLALTSLHPRAIWQNYKKRIDQASIEFLNLASLMQQQLH
                     KNSQQQSEAFHTMQYLLQSKLHQYQQNLDEMTLTLPSEMAKKFKETENRISMLELQLE
                     SVSPKHMLEKGYALVQKGEHIITSVQELTLEEELSLRMKDGSAEVKVQDIYPELDNGN
                     SSLEQ"
     gene            32305..33771
                     /locus_tag="PK015_02535"
     CDS             32305..33771
                     /locus_tag="PK015_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOL33384.1"
                     /translation="MTEDRSKQAGILTTAEFIRFGFKTIIGIVLARILLPVELGSYRQ
                     LFLIYSTFSTLLLLGIPQSVLYFLPKLEHIDSKKEFITRTVNLITLLAFVFGLAIFLL
                     RGFIAHIFNNPQLNILLIIFAVYPLFMFITQIYSQIMLGLKQPKKTATFTLISVACDF
                     IFILGTALITRNLHYIVLGVMFSALLQWGYAQYNLGRYFTKVTFDPDFYREIFKYSLP
                     LGLASIIGMLSVQLDKFVVSGFFTPAAYAIFSIGAIELPLISILNNSVNAILLPHISS
                     APDQIYTIYRASVRKNALIIFPLCALGLIFAEPLITLLYSSTYKASAPIFQVYLINLP
                     LRIATYGIIFMALKKTKYIMINSIITLSMNLVLNIILVKLIGMIGAAISTVIVSWLSV
                     AIYLYWIKNSLKLSLTELFPLPAIGKTALAVTISALISLSVLWIFGKSWIFQIVALVV
                     FIIAYFLSGKALNAILPYDIQYIKQLLNQAKLKLFGTK"
     gene            33772..34518
                     /locus_tag="PK015_02540"
     CDS             33772..34518
                     /locus_tag="PK015_02540"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOL33385.1"
                     /translation="MDYYSEHTWKHYDSEGRSLERLACIKKMIPEDVKTILDAGCGNG
                     IITNELARDYEVTALDPSAIALQYVQCAKICASIDAIPFPDNSFDLVCSNEVLEHLDD
                     ITLQKGIQEIKRVSKKYLIIGVPYKEQLEKKFYRCAVCGFSENVYSHLQSFDLSKLDI
                     LLTPEFYRIEYHIFGPMERNIHPFLLSILHKQGQWFLPSEGQKCPQCGSTEFLHKSNL
                     LTRMVNGMNLIFSHPHPYWLYTLYQKIDNI"
     gene            34518..35831
                     /locus_tag="PK015_02545"
     CDS             34518..35831
                     /locus_tag="PK015_02545"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOL33386.1"
                     /translation="MKTVLYIVFYYPPVGGSGVHRGVKFTRYLPEFGWDSIVLTPNPK
                     LLKQPKDYSLLKELSPEQKIEHFFTLDARWLFKIFWGLNLPQVVNWLNYHIFKPDEDI
                     ISLPFLHRKISSLMKRQKIDLVFISGPPFSLMLNVLWLKKNYPLPVITDFRDDWSQGQ
                     SRLDNPPPESFKQYELQLEHQVLAKSDKVIVANEAIKDNFLSIHSEMSPEHFVVITNG
                     YDEKDFQKDFPPRETEKDKLQIVYIGSLYGRRQPAMVWKVLIDLVEQGKINPEKISIH
                     IYGPNYRSFVFKGFENNQILEKIVKLHPYLNHSQVPEVMSQADVLWLFIGAGPKSDAI
                     STGKIFEYMRSGKPIWAVINPQGMAANILRPSGLSFIANNENPVDISNSLVNLYNLWQ
                     EEKLAVMPNWDYIHSFERRELTRKLAGVFDQTLSKINPDKMNNLD"
     gene            35851..>36836
                     /locus_tag="PK015_02550"
     CDS             35851..>36836
                     /locus_tag="PK015_02550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOL33387.1"
                     /translation="MKNKVLLLLPTHSSFMKVDEEILKSAYEVKTIYLQQDVSRKRYF
                     QMIVRALLTLIFDHSYQKVLIWFADYHSAPLVLLSKWKKIPSFIFIGGYDAVKYPELG
                     MGVYCSRVRGWCAKIALKNCTHIISNHIALLSSNNTYYKPEGHKDGIYNLIPGLKTPA
                     SVIFNAVSISPPAIISEQRLRQMVTAGSTPRFQDFYNKGFDLLTDIAQRRPDMSFVFV
                     GIDKKWLPELNKIYHFENLPNITIYPYLPQKEFISLLELSSVYAQPSISEGMPNSLME
                     AMLAGCIPVGSNVAGIPTIIDKYGYIIDHRDSQELEKALDLALETDADRKEIS"
BASE COUNT        11840 a         6707 c         6695 g        11494 t
ORIGIN      
        1 agaaattaat cctcaagtat atctggatat caaagataac caacccctct taatgtttag
       61 ttttaagtgg taatataaat tataaaattg attataactt aaatcagtta tcttcaataa
      121 atatattttc caatgctttt tccaccatct ctggtgtaat ctgtttcata cattcaaatg
      181 tccctttagg acatttattt cttccatgca aagaacaggg ctggcaagct aaatcagctg
      241 ttaaaacgat agctttatca ttcaaaggtg caaaacctaa acgaggatga gtccctccaa
      301 agatagcaat ctgaggtttt cttaaagcgg ctgctaaatg cataggaccg gtatcaccac
      361 ttataattgc gtcgcaggca tttatttctg cagttaattg cctgagattg agtttcccac
      421 aaagattttt tacatcatca aaaactgcat taagacttcg ggttaattca taatctgatt
      481 gattaccata aatcctaaat tcatattcat tatgacaatc ttcaataagt ttcttccagt
      541 tatctatttg ccaaattttt gtattatgag ctgcaccagg gaataaagcg ataagttttc
      601 tgtctggata aatgcgagga gtaatctcta tctcttcggg aattagttca ggatgaagca
      661 cgggatagcg tatagaataa gtggaactaa atttatccgg aaagagtttt tctaaagcgg
      721 aataatataa tctaaccgtg gattctatgc tgatagaatg attccctctc acaattcttt
      781 ttcttaattt atgctgttta ttataaattg ccttcttgtg agagcgaacc agattgcgaa
      841 taagccaggt ggaaaactta ttatgcaagt ctatcactaa atcgtaggat tgtttagtta
      901 gattaatatg ccaggaaaag cttttagaat agggaattat ttttaaattt aagccaaaaa
      961 gttcgggtaa agcttgatat tctggtttac agagaaaata gatttcagca tcggggaaaa
     1021 gttctcgcaa ttctgctact acagattgag tgaggataat atctccgata gaacttaatc
     1081 ttataaccag gatacgcatt ataaggtgct tctgataata tcgggaatac gaggcataag
     1141 ctccgaaagc ttttctggat tttttcctcc tcccatagca gaatcagctc ttcctccacc
     1201 tttaccattg agttcttgag atacagcttt aacaattttc ccggcattaa atttatccat
     1261 tatatctctt cccactacac aaagaatagt aattttatcc ccttctattg taaaaagtag
     1321 agatatggtg tctttggctc tgtctttaag caaatctcca agttctctta atctttccgg
     1381 ttcagtttca acggttgtgc aaaggagact aaaatcggga tattctacta ttttatccag
     1441 taatttatcc acatcagcaa aactttgtct ctgctctttc tttttaagct cttcttccaa
     1501 ctctttaatc agattttgca ttgtctccaa ttttgtttcc agcattttct cagatacatt
     1561 tagcacagcc gcaagatttg ctatcctttc ttgaagagaa cgcacaaatt tctctgcacc
     1621 tcttcctgta acagcttcta ttcttcggat tcctgctgag gaagagcttt cggaaagtat
     1681 tttaaaaagt ccaatctcac ctgttgcaga aatatgagta ccaccacata actctttgga
     1741 aaaatcttct acactaataa ctctaacttt ttgaccatac ttttcgccaa aaagagccat
     1801 tgcaccttca gagcgtgctt catcaatatc tttaatctca atatggacag gtctgttctc
     1861 tttaactatc tcattaacta tatcttccac cttatttatc tcatcttgtg ttagtgcttg
     1921 cagttgggtg aagtcaaaac gaagataatc cggaccaaca aacgaaccct tttgttgaac
     1981 atattcccct aataccattc ttaaagcttt atgcaaaaga tgggtggcag tatgattccg
     2041 agctatatct ttccttcttt gaaggtcaat ttctgcttta actaaaggtg aagatatagc
     2101 acctctaacc agatgaccaa tatgaataaa cttatcatct atgcgtttaa catctattac
     2161 ctgcatctca aaatcatcat tgtaaattct tcctgtatct gcaacctgtc ctccagattc
     2221 agcataaaat ggagttttat caagttgtat atgtaaattg ccttcatcat caatccgata
     2281 tctttgaata tgtgcttcta tgctaaaatt atcatatcca acaaaatctg ttggagtaga
     2341 gggcattaat tctatccaat ccatatcttc ccatttatag ataaatttag agcttttccg
     2401 agctcgttct ctttgttttt tcatctcttc ttcaaaacca gcagtatcaa ccttaaaacc
     2461 tctttcttct gccagattga tagtcaaatc aagcgggaaa ccataggtat catagagttg
     2521 aaaggcatcc ttaccagaga taatcttttc tgcagagttt gcacaaatct gatcaaatct
     2581 ctccagacct ttatccagtg tagaattaaa acgctcttct tctgctttaa ttaccatctt
     2641 aatataggct tctttacctt tcaattcagc aaaaggttca ctcattattt ctaccacaga
     2701 atcaactaaa tgaaacataa aaggttcaga aaatcccagc aatcttccat gacgagcagc
     2761 tctacgtaaa atccttctga gaacatatcc ccgtccctca ttagaaggaa aacctccatc
     2821 agcaagagca aaacaaagac agcgaatatg gtcagctatc actctatgag acataccagt
     2881 ttcaggatta taaggaaccc cactcatttg gacgatttta tctatgatgg gcataaataa
     2941 atccgtctca taattgctat aagtattctg gagaatttgc actaaccttt caaaaccagc
     3001 tccagtatct acataacgag atttcaaagg cgaaagtgaa ccatcttctt ctctattata
     3061 ttgaatgaaa accagattcc atagctcaat ataacgatta caatctccat tcaattcaca
     3121 tttatgacca ggtacattct ttttcatgca atgttctata ccgcggtcat agtgaatttc
     3181 ggtgcaaggt ccacagggac ctgtctcacc catttcccaa aaattatctt tatctccaaa
     3241 ataggatata tgagaggaat ctatatcggt attatttttc cataaattat agctttcctc
     3301 atcagaatta tggaccgtgg caaaaagttt atctttaggt aaatggaata cattagttaa
     3361 gagttcccaa gcccattcta tagcttcttt tttatagtaa tctccaaagg accaattgcc
     3421 cagcatttca aaaaaggtat gatgatagcc atctttacct acagcttcta aatcgttatg
     3481 tttgcctcct gcacgaatac atttctgact atttacagct cttttaactt ccggttcttt
     3541 taatcccaga aagatatttt taaactgatt cattccagca ttggcaaaaa gtaaagtggg
     3601 gtcatcccag ggaattactg gagaagaagg gataaaggta tgccctcttt ccctaaagaa
     3661 atctataaaa gtctgacgaa tctctttact agtccacact taattttgct ccgttttttc
     3721 taaagcttca taattatctc taatccattt ttcagcactg aaagcagcta tagcaccatc
     3781 actagtagct gtgaccactt gtctcaaaac tttatgacga atatcccctg cagcataaac
     3841 accaggaata ttagtatgca taaattcatc cgtgtaaaca aaacctgctt catcaagatt
     3901 aatcctggat tccagcagtt tattgtttgg taaaatccct acataaataa aaactccatc
     3961 aacaggtagg aagcttatct cttgagtaac ccgattaaaa atctccagtt tttctatttt
     4021 atcagaacca tgaatctctt gaaccactga attccaaata aattcaatct tgggattttg
     4081 gaaagcgcgt tcttgtaata tcttttgtgc tctcaattca tctcttctat gtatgatgta
     4141 aaccttacga gcaaagtgag ttaaataaat accttcttct actgcagaat ctcctcctcc
     4201 tataacagct acaactttat cacgatacaa agcaccatca caggtggcac aataggaaac
     4261 acctcttccg gtaaagcgtt gttctccagg aacatttagc cttctcggat aggcaccagt
     4321 gcataagatt aaagcctttg tgcggtattt gtgctcacga gtttccacaa ttttgcagag
     4381 accttccatt cctattgccg taacctcttc atctaaaaac tttgcttgaa atctttccgc
     4441 ttgttgtctc attctttctg taagctcaaa gccttctatg gcatcaggaa agccaggata
     4501 attctccacc tgatgagcag aggtaatctg acctccaatt atagctcttt ccaggataat
     4561 agtatttaat cctccgcgtg ctgcataaat tcctgcagct aaaccggcag gaccacttcc
     4621 tattataatt acatcatatt gcattttcta cctcattacc aaaagatact ttataccctt
     4681 tttaacacta agtattaaag tcaagaattt tatcaaactc attaaatata gtcatttctt
     4741 tgaatacata aaagttaacg agataaaaca aagtaaaaga aacataatgt atcctttgtc
     4801 gtaatattta atatgacgca tagattgttt atcatttaat tatatttaat tttaatatgt
     4861 actatcatta ccgtcctaac acctatttta gagaaagtaa tttatccatc attaaattcc
     4921 aaatatcttc cactttatct tcaacctatg tccagcttat gtccagctta tgtccaactc
     4981 atgtccaact catgtccgaa atctatcata agctcaacat aagtgagaca ttaacttgat
     5041 ataaagacct aaagagatag aactatcaaa gttagtgata gatacactat gtttatgtcc
     5101 gaatattttg atgctacacc ttaagttaag caaaagtaag gaatacttaa aatccataat
     5161 taatgaggat taattaaatg agtaaaagaa cttgaccgat aaatggaaat taagaatttg
     5221 gaggtaaatt aaattgagga gaagataaat gaaagtaata accgttaaaa ttaagattca
     5281 tccgcaatat ccgatgtttc aacttgattt caatagcagt ttaatcctca atcttaatta
     5341 taatgatata attttaagga attatgtaag ttatggatta tagggtctca gtagtacagt
     5401 ttaaacctga gtttttagca ataacagaaa atcttaataa attgaaagct atactttcag
     5461 agatggagac agatttagta gttttaccag aactctgtct atccggttat gtattccaga
     5521 aacaggaaga ggttgagaaa gttagtgaga ccattccgga aggatttgtc tttcaggaat
     5581 ttcgccaact ttctactgaa aaggaattca gtatagttta tggttttgca gaaaaagcag
     5641 aaaataggta ttataattca gctgctctga taaatccaga tggcagttat tatatttatc
     5701 gtaaaaccca tcttttttat agagaaaagc tcttttttac tccaggagat actggatttc
     5761 aagttttccc tgctaaaggt aatattctga taggaataat gatttgtttt gattggcaat
     5821 ttccagaatc tgccagaact cttgctctta aaggtgcaca aataatctgc catccttcca
     5881 atcttgtttt accctggtgt caacaagcta tgaagacccg ttccttagaa aaccgggtgt
     5941 tttccatcac atccaatcgt gtcggtattg aatataataa cggtgaaact atggaattta
     6001 ccggaaaaag tcagatttta ggtaccaaag gagaagttct gcttcagctg gacgaaaaaa
     6061 atgaaggaat tggcacaata attattgacc caaaccttgc tctggacaaa tcggttaatc
     6121 ctcttaatga tgtttttaaa gaccggagaa gggagatgta tgaattatga atgaggaatt
     6181 gaaaaaagag atacaaaaac ttagagctga aattgaacac cataatgaac tctattataa
     6241 actggcaaaa ccagagatat cagattatga atatgaccag ttggttttaa gactacgtca
     6301 actggaagct caattaagtg cggaagaact taaagaatcg ccactggaaa agataggaag
     6361 tgatttaacc cctggagctg aaaccatacc tcataaacaa agaatgtaca gtttggataa
     6421 cacatactct gctaatgaat tgaaagattg gatatataaa ctggcaacag atctgggatt
     6481 cttccccact ctttgtgctg aattgaaaat agatggtttt tctatcaatc tatattatga
     6541 aaatggtgtt atgcaatatg ccaccacccg gggagatggt attgttggag aagtagttac
     6601 gaacaatgtc aaattgctta aggatattcc tcataagatt aatttcacct atcctataga
     6661 gatacgcggt gaaatatata tgcccgtaga agaattttta aagcttaatg aacaacgtag
     6721 agccaatgaa gaaaaaccct ttgccaatcc cagaaatgca gctgctggct ctataaaatt
     6781 gaaagatcca gaagaagtta aaaatcggaa tctcagagct atattctata gcgtgggcta
     6841 ttgtgaaaat ttaccagtac aaaggcaatc agactttctt aattggttag atagtttagg
     6901 tttccctact gcaaaacaaa gatgtgtctg taattcttat gatgaagtgc aaaaattttg
     6961 tgatgagtgg gaaaataaac gctatacctt gccttttgag attgatggaa tcgtagttaa
     7021 agtggatgat tttgctttac aaaggaaatt aggctacacc gctaaaagtc ccaaatgggc
     7081 tgttgcctat aaatttaagc cagaaatgaa agaaactcgc ttgattgaag tgcaatatca
     7141 agtcggaaga actggagcag taactcctgt ggcaattttg gaaccagttt atatttcagg
     7201 aagcactgtc tctcgtgcta ctttacataa tgaagatgaa attagacgat tggacctgca
     7261 tgaaggagat accgtcctcc taatcaaatc aggtgagatt atacccaaga ttcttcaagt
     7321 tatacctgaa aaacggaaac ctaacgcaaa accagtgagt ttccctaccc agtgtcctgc
     7381 ttgtggaagt cctttacaaa aagatgaaga aggtgcaatt cattactgtt ccaatgttgc
     7441 ctgtcctgct caacttcagc gccgtataga acattttgct tctcgtgatg ctatggatat
     7501 aactggattg ggagaaagtt tgatagcaag acttttagaa aataaactca tctctggtat
     7561 tgctgacctc taccgattgg actatgataa attggcacag ctggaacatt ttggtcctaa
     7621 atccgctcag aatctcaaaa atgcaattga agcttctaaa accagaaatt ttgatagagt
     7681 actttatgcc ttgggcatta gatatgtagg aactgttacc gcaagatact tagctgaata
     7741 ttttggagat atagattctc tacgccatgc cagtctggaa agtctttctc aagttccaga
     7801 agttggagaa aaagttgctt tggctattaa agcctggttc agtctacctg aaaatataaa
     7861 attgatagag gagcttcgtt ctttgggaat taatttcacc tatagaagaa ctcaagcctc
     7921 taatgctttg gcaggtaaaa cttttctcat aactggaact ttacctcatt attcccgaag
     7981 agaaatggaa gacttaattc aaagtcatgg tggaagaatc attagtggag taagctccaa
     8041 tctggattat ttaattgtgg gagaaaatcc aggttcaaaa ctaactaaag cacagaaaat
     8101 acccacaatc aaaataatca ctgaagatga acttctggag atgataaaac agtgaaaatc
     8161 ctgtcccgct acatcctgaa agagcatctc agtccctttt tcctctcgtt actcgtggta
     8221 acttttgttc tgctcataga tagagccata gatttaatga atactattat tgaaaagaga
     8281 ctggatattg gaacggtatt acagcttttt ggactatctt taccctatat gctctcactc
     8341 tccattccca tggctgtgtt agtggcaact attcttactt ttggaaaatt gactgttgat
     8401 agagaaacga tagcaatgaa agccagcgga atcaatatct atagtctgct tggacctctt
     8461 tttatcgcag caatactttt taccgggcta atggtttatt ttaaccacta ttttcttcct
     8521 aatactaatc ataaattaaa gaatctcacg gttaaaatag cctattatag accgatgacc
     8581 atcataaaac ctaaggaatt taccaccatt tctgattata ctatctacac ggaagaaaat
     8641 ttaaatgatg agctgaaaaa tgttcttgtt tatgaccgaa cgcaagccaa ttttcctcgt
     8701 ataataactg ctcaaaaggg aagaatagtc caaatggata aaggtaatag ccttcaggta
     8761 attttacaaa atggagaaat gcacaatcgt aatgaacagg aaaaagggaa atatcaggtg
     8821 actaaatttg agacttttac agtaaatatt agagatttag ctgtgggaat ggattttggt
     8881 gaaagtggtt atcgttctga tagggaaatg acctataatc agttagttgc agatataaat
     8941 aataaaaaac aggaacttac aaatcaaaaa ctggaaatta ctaatctgca aaaaagattg
     9001 gaagaattgc aaaaacaacc tgatgattat gccaaaagtg tggaaactcg tcgtctgagc
     9061 attatgaaaa gtatggcaga agaccagtat aaagaaattc tggaaaatct ccgttcactt
     9121 caggtggaat atcacaagaa gtttgccctt gcatttgcta taattatatt tgttatgatg
     9181 ggtgtaccta tcggattgat gactcgttcc agtggaattg gaatggcttt ctcggtttct
     9241 tcagttatct ttctaatcta ttatatagct ttaacaggtg gagaacaact ggcagataga
     9301 gccttaatga gtccttttct ttcaatgtgg ataacgaata tcatcttctt cattatagga
     9361 ctgtggctta tccatctttc tatccacgaa aagcaactaa ttaacttgaa ccttcttcag
     9421 tggaagcttt ctcatcgtaa agctaaacct gaagaaactc cagatgaaat aatacattaa
     9481 taaactaaga tgcgtatttt agaccgttat atcgttcgtg aattcctcaa gacctatctg
     9541 gttatctttg tctgttttgc tgctgtgttc accgttattg atgtcataga taatctgcct
     9601 aaattgataa gaaatggtgc cactgttgaa caagcgatag aatactatct acttcgttta
     9661 ccttatctgc tggtgctcac ctctcctgtg actatcctga ttactggtct attcttgatg
     9721 gatagtcttt ccaaacacaa tgaaagtgta gccattcgtg ctgctggaat cagtattcat
     9781 cggactatgc ttccactttt cctggttgga ctaattatta gtattatagt tgcacttttt
     9841 ggtgaatatg tagttccctg ggcggaaaaa actcgtaact atgtgtataa tgtggaaata
     9901 aagggagaac cagaagaaag taatatgctc aaagccagag ttcattatcg gggcacaaaa
     9961 aatgatttct attactttgg tttctttgat ggatacaaca atactttacg ggtgatagac
    10021 ctcgcccaat taaatcctga aagtaaagag attgctaaac atattactgc ttctgcggca
    10081 gtctggcaaa atgataaatg gataatccag gattgtgaga ttagagattt tggacaggga
    10141 cagcagacac aatatcaata ctatccgagc actgaccttc ccttgttaga tgtgcaacct
    10201 aaagatttta ttcgcattac taaaaagact ctttctttaa acttttttga actaaaagat
    10261 tatattgccc gcttgcaaaa aatgggtgaa aaagcacata aagaaattgt agatttacat
    10321 atgaagattt ctttccctct aaccaatctg attgttatct tcttctgtat ccctatcgct
    10381 actactaaca taaggtctaa gggtaggggt ttaatcttcc ttctcggttt agttgtctgc
    10441 tttatctatc taatttttgt tagagtcttc cagagtcttg gttataatgg tgttattcct
    10501 cctgttttag ctgcctggtt tcctaatatt ttcttcacta tactcggttt gttcttcttg
    10561 cataaagccg agatttaaaa aaagcttgaa taaataatgc taattaaaat aattgctttt
    10621 ttaaaacaat atttaaccct caggagttaa tagatgcttg accaacttgt aaagaaacag
    10681 aaagttataa tctatataat tgctattgct tttatactaa gcattggtgc tggaggtatt
    10741 tttggaggag aaaaaatcat ccaggcttta ggtggcaatt atttagggaa agttaatgga
    10801 actaagataa ccccccagca atacaatctc aagattcaag aaattaaaga acgctatgaa
    10861 gctcaaggac agaaaataga tgaaagctat atgccctata ttcagaacac tgcttgggaa
    10921 gaactggtca atgaaatact atggcaacag cagataaaaa aacatcgcat taaagttacc
    10981 gaagctgaaa ttaagaatgc catagaaaac gatattcctc aagaactgct gcaaaatgaa
    11041 agtcttcaaa ccaacggtgt ctttgacaaa aagaagtatt ttcaagcttt gaataataat
    11101 cctcagttta aactgcagat gtatgaatat atgaagacct atctaccccg gaaaaaactc
    11161 cagcaaaaaa taatgagtga agctggaata acattggaca gcttgaaaca ggaatttatc
    11221 aaagaaaatg ataaagttta tggtaaaaca atctggttct cctatcacaa agctgattcg
    11281 gtttatgtaa gcgatgcaga aatcaaaaaa tattatgaag aacacaaaga taccgagttc
    11341 aaaaagggac ctgcttcccg gattaaatat attactatcc ctatccaacc ttcagaacgc
    11401 gattacaatg aagtcaaatt ggaaatagac gaaatctatc gtatggttaa tccagaaaac
    11461 tttcctatca tcgctgaaca atactcagaa gatgaaagtt cagcaaaaca gggtggttct
    11521 ctgggagtct ttggaaaagg tcagatggtt ccagaatttg agaaagtggc tttctctctc
    11581 aagcctggtg aaatttctaa acctttcaag acagattttg gctggcacat tgttaagtgt
    11641 gattcaatta tcaacgatca gattaaagcc agtcatatac tcttaaaggt taacacttcc
    11701 gatgaaacaa aaagagaact ctacgaaaaa gctaagcaag cacgcaaact aatcgctaaa
    11761 aaaggaatag atgccgcagc taaagaactc aatctggaag ttataaccag tgactggtta
    11821 cctcatgacc aacaacaaat cccaggaatt ggtcagcatg gtggcttaca tcaatttatg
    11881 gtaaagaaaa aacctggaaa ggtttctgag gtataccaat taaatattca gggtaaacct
    11941 tcctatctta tagctcaggt ggtagaaaat cgtaaattct actatgagga ctttaacgca
    12001 aaaaaactcc agataaaata tgacctggaa agacagaaga aaatagcgct gatgaaagct
    12061 aaggctgaag actttataaa aagagttcct aaagataaat actttgatgt tgccgctgct
    12121 gaaggatggg acatcattga attaaaggga cataaaaaag attcccgttt acctgcacca
    12181 gtaaatgcaa ccctggaaga atttgataaa gctgctctgg cactaaatac gggtcaatat
    12241 tccgatattg ttaacaccaa agagggtcct tttattatct atgctgaaaa acgcgaaaaa
    12301 ccagatatgc aacaattcac aaaagaaaaa caggaagaga ttagaaaacg actggaagag
    12361 gctgccttta atcgctggtg gcaacaaatt cgcaaggaag caaaaattat agataaccgc
    12421 tataaatacg gatattatta aagctctatg agcaaattaa tgaccagtgt cagcggtgtc
    12481 cgcggcattt ttggtgaaac tttaaatcca atagtggctc tgcaatatgc gagccacttt
    12541 ggacaattta tagctcaaga atcagctaat cctgcttctc atcatatccc aaaaatcgta
    12601 gtaggaagag attctcgcac cacaggcaaa gctatgctct atgctattct cagtggttta
    12661 attagcactg gatgcgaggt tattgattta ggcatagttc ccactccaac cgttctactt
    12721 aatgtaaaaa aacattctgc tcaaggaggg ataagcatta ccgcatctca taatcctcct
    12781 caatggaatg caatgaaatt tgttgatgga gacggaatgt ttctatcacc agaaaaagca
    12841 tcccgtttta ttgaatctgt taacgcagaa attagctgga atgactggca aaatatggga
    12901 acagtaactg aagatgccga agccattaac tatcacattc aaagaatcct tgctatcccc
    12961 tatctaaata ttgcacaaat tcgttccaag aaatttaagg tcgtacttga ttctgtaaat
    13021 ggagctggag gcttagcttc tcccttacta cttaaagctc ttggctgtga agttgtagaa
    13081 ataaattcag aacctacagg agtttttgcg cacacgccag aacctctaaa tgagaatctt
    13141 catcagctgg aagaaaatgt taaattacat aatgcagatc ttggttttgc tactgaccct
    13201 gatgtagacc gtttagctat tgtagatgag acaggacatt gcattggaga agaatattca
    13261 gtggctttag cagaactctt tatcctacct aaaaaacctg gagatatagt agttaatctt
    13321 tccagttcta tgctatctga ttatattgcc cataaatttg gtgttaaagt gcatcgcacc
    13381 agagtaggtg aaatttatgt gggcaaaaag atgaaagaac tacattctcc tatcggtgga
    13441 gaaggaaatg gcggtattat atgtcctgaa gttaattata cgcgtgatgc cttaactggt
    13501 atggctttaa ttctggcact tcttgctgaa acaaaccaaa agctatcaga gattgtaact
    13561 tcactgcctc gttattatgt tgctaaagcc aaaatagaat tacctataga acaaattgat
    13621 aaagctctaa aaaagattcc ttcattactt cctgattatg aactggattt tcaagatgga
    13681 ataaaagcca ttgctccaga ccactggatt catataagaa aaagcggaac agaaccagtt
    13741 atacgcatct atgcagaaag tgattccctt cagcgttcgg aacaactttg ccaggatatt
    13801 ttaaataaat taaattcctg agtattatct ttctcttcta ttcttagtaa ttacttctct
    13861 taaccctaaa tcaagattca tcctggagtc aaaaggtcaa tttaaagaac aaaatagagt
    13921 tgaccaaaac cttgcagatt ttaaaatgta aaaaaataat attaatagga ggaaacaatg
    13981 agaaagactg ttgtagtaag tgccaaacga actgctattg gcaattttgg agctgctttc
    14041 aaagatgttt ctgcagtaga cctgggagtt gctgttctaa aagctatgct ttctgaaaca
    14101 ggaattaaag gtgaacagat tgatgaggtc atcattggta atgtctgcgg agcaggattg
    14161 ggacagaatg tggctcgtca aatctctatt aaaagtggaa ttccggtaac tgttcctgct
    14221 tatactgtaa ataaggtttg cggttccgga atgaaatcag taactttggg agctttaatg
    14281 ataggaacag gagaagcaga tatcattgtt gctggcggaa cggagaatat gagtcagata
    14341 ccttatgctt caccagatac cagatggggt tgtaaaatgg gaaatcgtaa tctggtagac
    14401 ttgatgattc gtgatggctt aagcgatatt ttcaatgatt gtcatatggg cttaacggca
    14461 gaaaatctgg cagaaaaata tcacataacc agggaagaac aggatatctt cgctgcgcaa
    14521 agtcagaata aagcagaagc tgccatcaaa tctggtcgtt ttaaagatga gattgttcct
    14581 attataattc ctcaacgcaa gggtgaccct ttagtgattg atacagatga aatgcctcgt
    14641 ccaggtgtta ctcctgaaag tttgtccaaa ctccgtcctg cttttaaaga tggtggaaca
    14701 gtcacagcag gaaatgcttc tggcatcaat gatggtgctg ctttacttct tcttatgagt
    14761 gaagaaaaag caaaagagtt aggacttcct attatggcaa ctttagttaa ttatgcttct
    14821 gctggtgttg atcctgctta tatgggatat ggtcctgtgc ctgctattgg taaagtgcta
    14881 aaaaaatctg gctggaactt taacgaccta caattgatag aactcaatga agcatttgcg
    14941 gctcagtctt tagccgtttt caagggtctg gaagaagaag gaatcggaaa gattgattta
    15001 tcaatcacca atgtcaacgg tggagctatt gcgctgggac atcctatcgg agccagtgga
    15061 gctcgcatta ttgtttcttt actatatgaa atgcaaaaac gggatgtaca taaaggtctg
    15121 gctgctcttt gcattggtgg tggaatgggt attgcttctt tatgggaaag atagggagta
    15181 aataaaatgg cagataaact aaaattaaat aaaagtatgg ctctctttga ggaatcacaa
    15241 aaagtagtgc ctggcggagt tgccgggatt agaagacctt acaattttgt accaggtgaa
    15301 taccccatct tttttgagca tggtaagggt ggacatatta cagatgttga tggaaacgaa
    15361 tatatagatt atttatgtgc ttatggacct attattttag gttatcggga agaagaagta
    15421 gataatgcag taattgaaca aatccgaaac aaaggcatct gtttttctct cactcaacct
    15481 gttcaaactc aattagctca aaagcttact gaaattatac cttgctgtga aatggcagct
    15541 ttcgttaaaa ccggaagtga tgctaccact attgctatta gagttgcacg agcttatact
    15601 aataaagtaa agattgctcg ttgtggttat catggctggc acgattggtg cgtagaagtg
    15661 aaaactggca tcccagaaaa actctatgaa gatatcctgg aatttcatta taatgacctg
    15721 gataatctgg aagatattct caaagctaat gctaatgata tggcagcaat cattattact
    15781 cccgttggac accctttagg aaatcctgta gaaatgccaa aacctggcta tctggaaggt
    15841 gtgcgtcaac tggcagataa atatggttgc gtgcttattt ttgatgagat tagaagcgga
    15901 tttagatgtt ctttgggtgg tgctcaagaa tattttggag ttacacctga ccttgcaacc
    15961 atagggaaag ctatggcaaa tggctatgct attgcagctc tggtgggaaa gagagaaata
    16021 atggatgtgt taactaagga tgtattcctt tcttctacct tctttcccaa ttctgatgca
    16081 caggtagcag ctctgaagac catagaaatt ctggaaagag ataaggtcct ggaggttatt
    16141 gcagaaaaag gaaagaagtt tggtgcggaa gttcaaaaat tggtagaaga atctgctctt
    16201 ccgattcaat ttactggtgc accctggatg ccttatataa cctttaatcg tgaccccgaa
    16261 ggtctttaca aaaagcttcg taatgaattc tacactcagc ttatacgtcg cagagttttc
    16321 ttacaacctt atcatcatgg ttatatctgt tatcgtcata cagatgaaga ccttgcctac
    16381 accgttcaaa ctattaaaga atcgctggat gaattaaaga aactggtgta gctaatatta
    16441 aaagaaatat ggtgtcctgg aggtagaaaa atggctcttt tctttaaaat ttgcctccag
    16501 cgacgccttc taatttttat atagaggata aatggcaaaa tatattttcg tagtaggtgg
    16561 agtgctttct tctttgggaa agggtatcgc tactgcttcc attggattat tgctcaaatc
    16621 tatgggctat aaagttgtcc tgcaaaaatt tgacccctat ttaaatgttg acccgggcac
    16681 tatgagtcct tttcaacatg gcgaaatatt tgttactgat gatggagcag aaacagacct
    16741 ggatttggga cactatgaac gttttaccga tgtatctttg acccgtcatt ccagtagttc
    16801 tgctggtcaa gtctatgaaa cggttatcca gaatgaaaga agaggtgttt accagggaaa
    16861 aactgttcag gtaatacctc atataaccga tgaaataaaa catagaatac gtgctctgga
    16921 agctgattat gacatcatta taacggaaat tggtggaact gtgggagaca tagaaagtct
    16981 tcctttcctg gaagcggtgc gtcaattaag acttgattta ggatataaaa ataccctcta
    17041 tattatgcta acctatgttc cttatattaa atctgctggc gaactgaaaa ctaaaccttc
    17101 ccaacacagt gcctataaac ttagagaaat aggaatccaa cctgaaatct tgctctgtcg
    17161 ttcagaaaaa cccttcggta aagaaatata tgataagatt gcccttttca ctaatgtccc
    17221 acgagaaaat gtcattaatg ctatagatgt taaaagtgtc tatgagtgtc ctatcacctt
    17281 taaaaatgcc aatctgcaca aacttatctg tcagcacttt gaaattgaat gcagacctct
    17341 taatcttcgt tcctggaaca aattcttaac taacagtcaa gactgtgtgg ataaggtcac
    17401 cattgctatc tgtggcaaat atgtccgtca tcaggatgcc tataaaagtg tagaagaagc
    17461 tttagttcat tcagctgcct ggcatcgtct aaaattacag ataaagtgga tagattcaga
    17521 acaaaatttc aaatctgcct tacttaatcg ttcacttcag gatgtagatg gttttttgat
    17581 tcctggtggt tttggcgttc gtggcataga aggaaaaatc gctattgcag aatatgctcg
    17641 cactcataaa attccctatt tcggaatctg tcttggaatg caggtagcgg ttattgaatt
    17701 tgcccgtcat atctgtcatt taaaagatgc aaatagcacg gagtttgacc ctaaaactcc
    17761 tcatcctgta attcacttaa tggaagaaca gcagtatgtt gaaatggtgg ggggaactat
    17821 gagactgggt gcttattctt gtaaacttaa acgaggaacc ttagctcgca aagcttatgg
    17881 aaaagtatta ataagtgaac gccatcgtca tcgttatgaa tttaataatg cctacaagga
    17941 aaaattggag tctaaaggac ttaccttcag tggtttttct cctgatggac ttctggtaga
    18001 aatcatagaa ttacctgctc atccttttta tatcggagtc cagtttcatc cagaatttaa
    18061 atctcgtcct aataaacctc atcctctttt ccgagaattt attagcgctg cttatcaata
    18121 tcatcaaagt cattcaactc cagtataaat cattctttaa agaactctgt ataaatttat
    18181 caacaatttt tggaatttta gtcaatttta attcttgacg aattagatat aaggcttaac
    18241 ttatttaaat attattaaaa gataagagtc tgtccgtgtt attaaggcaa atttactgta
    18301 gaactcatct atttatgaga agctctatta ctttgttaat aggtatagca ttaatttttg
    18361 ctggtttctg tcttagctgt gagaaaaaaa caagtcctga agaaaccata acttctcaag
    18421 aagaaaaatc tgtcccacct actcatttac ataaagttcg ctatactata aaatggcttc
    18481 accaggctca atttgctggt gcttatatgg caaaggaaat ggggttcttt caacagtatg
    18541 gtctggatgt ggagatcatt caagggggag ttgataatcc tccttataaa gctttaataa
    18601 acggtaaaac ggaattttgt agtatgaacc tgattacagc ggttgaaaga aacaatcctg
    18661 aattcccttt aatcaattta gctcagatat cccagaaaaa ttcctcctgg ctggtaggta
    18721 aaaaaactac tggtataaag aatctgcagg atttaaatgg tagaaaggtg ggagtctgga
    18781 gagatgaagc tggggatcat atccgctatt ttcttaatga taataaaata cgggcacaaa
    18841 ttatacctct ggattggtca gttaatctct ttctaaatga tgctattgat atgatgaatg
    18901 tgatgtccta taatgaatat caccgcattt tgatggcagg tatggaacct gaagatctcg
    18961 ttgtatttaa tctttccgat tacggttatg acttggttga tgacggaacc tacacttccc
    19021 gagcctatta tattcaacat ccagaactct gtaaaaactt taccaaggct ttaattgaag
    19081 gatggattta tgctctaaat catccagatg agacagttaa tgtggttgtt cgttatatgc
    19141 gtgataatca tctgcccgca aactataatc atcaatattg gatgttaaat cgtatgcggg
    19201 aacgaatcct ggaaaatcct gataaagttg gctatcttaa tccagaagat ttagctcttg
    19261 cagaagaaat tttaaaacga aatggtaaac tcgaatatcc tgttgagtat aaggaattct
    19321 ttctacaatg aaaacagaaa ctaaaccacg cactattgct ttccaaatta tctcctttat
    19381 gtttgtagtt ctggtcgcac tttttattat ttctattctt actactggca gattaatgca
    19441 tagcgttatg gaatctaatg cccgggaaag ctttggaaat atcactaaaa tcaatgtcca
    19501 aaccatagac caaactctct ctaaaatggt aatcttaggt cgtgacctgg agcttttgat
    19561 taaagacaat aatcatgaaa cagacttaaa tcttcatatc cgtcaacttc ttacagacaa
    19621 tccagaaatt gtctctgtat gtatagccta tgcaaataaa tctcatactt ttagtaatac
    19681 ctatttattg cagggtgcaa actttcttat aaatccctct attagtgatg atttttctta
    19741 taaagactgg tttttattgc ctaaattaac cgaaaaacct tactggagtg acccctggtt
    19801 tgattcagat gggacaggaa ccatcgtttg ttcttactct attcctttgt ttataaaagg
    19861 gaatgtagag ggtgttctgc gtctggatac ttcaatggaa aatctcaggc aaattgtatc
    19921 ctcagtaagg gtgaaaaaaa ctggctatgc ttttcttatt tccaataacg gaactattgt
    19981 ggctcatcct caagattctc tggctatgaa ttataccatt tttgacctgg cagaacagtt
    20041 aaatagcccc aaaattcgct ctattggaaa gagtatcgtc aatcgtaaat cagacttcgt
    20101 gcatctaaac aaaataggaa ttcccgctaa tgtctggatg ttctatcaac cagttccaac
    20161 taattcctgg tctttagcaa ctgtggcacc tgatagggaa gtttttgctg atttatacaa
    20221 tcttatgttt atttatattg gctgggtgct acttgctttt ttaatcattg ctattgccat
    20281 atggtacaga acccgaacca ttaatcaacc tctggaaaga ttaatccaat ctattgaaaa
    20341 atcacaatct ggtgttcctg ctactgcaac tttaccggac tcagacacct atgaaattca
    20401 ggttctaaat acaaatttca atcgcttgaa aaattccctt aaaaaatatg ctgaaagtct
    20461 gcaagaagtt acggcagaaa aagaccagat tatggatgaa gtgacctttg cttcagaagt
    20521 tcagcttaac cttatcccta ataatcaaaa atcatttacc ttaccaccaa atatttctgc
    20581 ctacggaatt ttggaaccag caggtttagt gggaggtgat ttatacgatt acttctctgt
    20641 tgatgatgaa catttccttt tcggtattgc tgatgtggtt ggaaaaggtg tagctgctgc
    20701 tttaactatg acaatggtca gtactcttct aaggacttat gcacctcaat tgaaacaacc
    20761 agaaaaaatc ctgacttata tgaataaatt cttgattgaa aataacccgg attatagatt
    20821 tgtgactatg ttactgggaa ttattgacct taaaactgga acttgcactt tctctaacgc
    20881 aggtcataca cctcttttcc tgctttcaga agatggaaaa ataactaaat acgctcaaac
    20941 tcattctaca gctcttggca ttctggaaaa cttaaatgcc agttctgaaa ctattcaatt
    21001 aaaatctgga gatgaaattg tcctttgtac cgatggcatc acagaagcgg ttaaccaaga
    21061 agataaacta tttggcaccg atagattgga aaaagtactg aaaaacttaa aaaatccaga
    21121 accagaatca actgctcaag ctattcttga tgctgtaaaa agttttgccg acccagaaag
    21181 acaaaccgat gatattacta tactggtagt taagtatatg aatgtaagtt aattagttta
    21241 tagcagaaaa taatataaaa tataggagta gaaattatga aacttagaaa aagcgaaatt
    21301 attagtttgg tgattgtaat aatatccctg attattggaa tctattttta tcctctaatg
    21361 ccagaaaaag ttgcctcaca ttggaatact gaaggagaag ttgatggtta tatgccaaaa
    21421 ttttggggat tgtttctgat gcccataatc tccgcagtat ttctatttct ttttatcatt
    21481 atcccaaaaa tagatccgtt gaagcataat attgaaaaat ttaggaaata ctttgatgga
    21541 tttatagtat taatgatggt gtttctattt tatctctata ttttaactat tttgtggaat
    21601 atggagatta gatttgtttt tacccaattt ttagtgccaa tttttagcat ccttttttat
    21661 tattgtggaa tcttaatcca aaatgctaag agaaattggt ttattgggat tagaactccc
    21721 tggactttaa gtaatgaaga ggtctggaac aaaactcata aaattggagg aaaattgttt
    21781 aaaatcgctg caatcattac tttaatcgga gtattttcac caaaatatgc tttctttttt
    21841 tgtattatcc ctgtaatttt agcttctcta tttccaattt tctattccta ttttgaatat
    21901 caaaagctat caaaaaattt aaagtaataa taaacaattt cagcgaaatt aggtgtgtga
    21961 tttaaggatt agtagtgaaa aagaaaaaca caggcaaaga tttctcaata gctaatttta
    22021 tttttgacaa cactaccgga caatgaaatt aataaattac aaaatcatct ggaatctgta
    22081 ttgatataca gtatttattt tccccagtaa aaaaacaaat cataagacca catttaaact
    22141 gacattttga atctttatta aatgtctccc cttacttatc cctgctatca agtgttaatc
    22201 aagcgtttat caagcgttaa tannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22321 nnatttccgt atatcataat atgattataa gagatagatt aataaataaa ataatctgta
    22381 tacaatatcc tgggttatac atataattac ttgattataa ataagttata tcatcataaa
    22441 agaaaaaatt cttgacaatt atctatagcc tttaaattta ttccttaaga ctaaaacaga
    22501 cacaggaatt caatatgaaa caacagaaag tgaaactcat cgctgtcgtt tttgctgttt
    22561 ggtccatatt ttgtggtccc attatagcgc aatcaataac tgaaattaca gcaaaagagg
    22621 ctgtggaagc tcgaacttca caggatgcgc tgattgattc tctgttaact aaagaaccac
    22681 cggatggaat aacagctcct gttatacagg aaacaccacc gggcgaaatt tggttagctt
    22741 cctattacag caaacgattt aacggtaaga aaacagcaag tggtgaatgt tataatcaat
    22801 atgcatacac ttgcgctcac aaaactctcc cctttaatac ccttctacaa attaccaatc
    22861 ctaaaaatgg caaaactgtc aatgtccgag tcaacgatag aggacccttc gttaaaggac
    22921 gcgatttaga cttatcctat gctgctgcca aagatcttgg cttattaaac catggggtaa
    22981 tgaaagttgt agtctcaatt ttgcctgaaa ctaatgaaaa tataaattta gctcttgaat
    23041 gaggcaagag cagtgcttaa agcacggaat caatccttac cccagctgtt agagtaaaga
    23101 ttgattccat tttttattat ggaggaattt atgttaccag catccatcaa aattgctccc
    23161 tccgttaggg aagctttaac agaagacaaa cctatagtgg ctctggaatc aaccgtcctc
    23221 actcacggtc ttccctatcc ggaaaacatt tctattttga aaactctgga aaatatactc
    23281 ctgagtcaag atgttacacc cgccaccata attgttttag atggaatcat ctatattggt
    23341 ttagaagaaa atattcagaa taaactggaa caaatgctaa agaatagaga aaacttcagc
    23401 aaattaggaa tgcgcgacct gcctttggca ataactcaaa aaagaagtgg cggaacgact
    23461 gtttcgtcaa caatgctact tgctcatctt tcaggaatta aagtttttgc cactggtgga
    23521 attggaggtg tccatcgtaa ctgggaaaat agtctggata tctccagtga tgtgattgct
    23581 ctttcccgga ttcctgtgat agttgtatca gctggatgca aagctattct ggatattcct
    23641 gccacccttg aagttctgga aactctggca atacccgttc tgggatggag aacaaatagt
    23701 tttcccttat tttataccgc taccagtgat tatcctattg aaagacttaa cgatactgaa
    23761 caatttgccg ctttctggaa aaatcatctt gcttttcaag ggaaaggggt tctcatcgct
    23821 aatcctatcc cagaagaata cagtcttcca cctgaaattc tggattctgt tatcaataaa
    23881 tcaatggagc aagctcacat taaaggaatc aaaggaaaag aattaactcc atttctactt
    23941 gactttatag cacaagcaac taaaggtgaa tccgtaaaag ccaatttagc tttgttaaaa
    24001 aataatgtcc agttagccgc agaattggct aagtctttga taaataaagg aagttaaatt
    24061 atgagaatct acatctccat tgatatggaa ggcatccctg gaacctttaa ttgggaacag
    24121 gaaaagacaa atagggcaac tatccaaaac tgtatgtttc atcatattca aaccgtcata
    24181 gagagcatac ttgccagtcc acaagcctct ttaatagaag aaatcaccct tgctgattct
    24241 catagtgcag gagataatct tgattacagc ataactgcac tggatgaaag aatcaatcta
    24301 atttctggct gtccccgtcc tcgctatatg atgccagatc ttgaccctgc ttatgatcag
    24361 gtatggtttt taggttatca cagtggtact ggtgctctcc gtggaaatat ggatcatacc
    24421 tattccaatt cccggataca caatatctgg ataaatggag aaagaatgaa tgaagcactc
    24481 atcaatgctg cttatgctgg atattcagga gttccagttg gactggttag tggtgatgaa
    24541 accttaaaag atgagcttct tctctatctt ccctggattg aatatattcc gactaagaag
    24601 gcaattgcta agttttccgc taagaactac tcgcaaaaac tggtagataa acagactaaa
    24661 tcctctgtac ttcagacact tacccgaacc aaagaagaaa tccctctctt cacttttgaa
    24721 ccccctttat cccttaaaat agaattcaat catagctcta tggctgacca agcacaattg
    24781 atgccctaca ctaaaagatt ggatggacgc actatagaat atcaaacgga tgattatgcc
    24841 atactatttg aggcaattat ggtgctggta tacctggcac aaatcactcc tatgtgatta
    24901 aaggaaaaaa ttatggaaga caatctggtc actattgcca agtttgaaga taattttgaa
    24961 gcggatttag cgcaaagatt attagctgat aacggtattc aagcattcgt gaaaaatgct
    25021 attttctatt ctatgatgcc tggagttttc cccggaaaag tggacttaga actccaagta
    25081 aatagcgaag atgaagaaaa agccagagct atactgagtg agtatcagaa tacacctgag
    25141 ataaaaagga ttttagtgag aaatgatgtc ctgcaagagg gacatttcct tctaacctca
    25201 ggacgccata gcgctaacta tctggaaaag atacatcttt atcagaatcc ccaggatact
    25261 gcccgggttt gcgaaatgct tgcagagtta ttggtagatt atgattttga taccgtggtt
    25321 ggacctgctt atggtggaat agttctggca tttgagaccg ccagaatgct ggaaaaggga
    25381 tttctattca cgcaaagaaa agaaggtgtg atgacaatcc gtagtggatt tgatttaagt
    25441 aaggtcaaaa aagcagttat tacggaagat ataattacca ctggtggctc agttaaagaa
    25501 gttataacca ctctaaaaaa tgcgggaata gaagttgttg ctgtcgcagc tattgtagat
    25561 cgtagcaccg aagaaatgga ttttggttgt cccttccttc ctcttttaaa aatggacatt
    25621 ccttcctggg aagctgatga ctgcgttctc tgccagaatg gaattccttt aactgtccct
    25681 ggatcaagtg ataagaaaat ctgaatttaa atgcctgaac ttgccattat aacccttaat
    25741 agacgagaac atagcgtcct ttcacacctc tggaaattct ttccttttga atctgatatt
    25801 cagtatcatt atctctatcc tggaaaagaa gatgaagaaa taacctctct tcgctctaat
    25861 gtccattctc ttgccttttc tggaaaactg gaagaagtga tagaagcaat ggataactat
    25921 ctaaaagaga acaatctctc tcatatctgc ttctccagtc tggaaactca ggcggtctgg
    25981 tcacgatatt ataaacaaaa tgctgtaact atttacacca gccaggacct atatctggct
    26041 gatattagtg ttctaccttc cgcttgtcca gaaaagcttg aggctatatc tgacttggta
    26101 aaacgagata tcgcagttct attaagtgaa gcaggaactt ttctcttgcc tgagaatacc
    26161 actatctatc cggattttaa tagttttacg cgaactatgt cccacgaaat cctgctaacg
    26221 aaaaataaag acctcgtttc agcagtctgc aaagcaagtg ttcgacatta ttttgttctc
    26281 catctaataa ataataaagt ttctcttaat ctttctctgg atacggaatc ttttgatttt
    26341 ggctatgtgt ttacgaactt gagtctcgcc accaaatgga ttaaagaagg tattgattct
    26401 gctcttcaag aaagaaataa aggttatctt ccctattctg tctttgccta ttactacgat
    26461 atatatatgt cccatgtgaa ttatgatgaa tggatagacg gaataatcat gtggtataaa
    26521 cagatgaatc caaatcctct gcacaaaatt ttggagctgg catgtggcac tgctaatgct
    26581 tcttctctgc ttgctgccag aggatttgag gttgatgctt gtgatgcttc accttatatg
    26641 ctccaggttg cagactctaa acctattaaa cctaacctct atcaagccta tctcactgac
    26701 cctattccga aaaaagatta tgacctgata ttttgtatct ttgacagcat caattatcta
    26761 ctccatccag aagaaatcag tacagtgctt gataatgtct atgaggcact tgctccgcag
    26821 ggcgtcttta tttttgatat ctccactctc ctgaatagta tggtcaattt ctatgatagc
    26881 acaaactatc tgaaagtcca cgatgcctat atcattcagc gttctaatta taatactttt
    26941 gccaatcacc agctcacgca tatcattatc ttccgcaaac acttccataa ttatgaactt
    27001 tctgaggaac gccacacgca aagagtctac tacactccag aacttataaa actctgcaat
    27061 gcttctaaac taaaactgaa agctatctat tctccagaaa agaaccataa cctactggga
    27121 aaaaatgtgg aaaaactgga taagaatttc tttcgtttat tttttgtgct tcaaaaggaa
    27181 agatgaatcc tctatacgaa tttgatagtg tctttttgaa aaactcagaa agaataattg
    27241 gagttgatga agctggaaga ggttctttag caggaccagt ggttatagct gcagttatcc
    27301 ttgatttaaa aaaccctata gagggaatta atgatagtaa aaagctcacg cctataaaaa
    27361 gagaaaaact ttttgaacaa atcatccagt ctgctattgc ctataaaatt gttgaagttg
    27421 atgtaaatta tattgataaa tacaatatct tgcaagctac tttaaaaggt atattagaat
    27481 ctgcttcagc tatttctcaa ccatttgatt tatgcctcat agatggaaat aaagtccccg
    27541 atggtctttt ctgcccaagt gaaagcataa ttcacggtga tgccctctct gcttccattg
    27601 ccgctgcttc tattcttgcc aaagtttatc gtgatagatt aatgagaaat ctggataaat
    27661 actatcccct ttatggtttt gctaaacaca aaggctatgg aacagctcaa cacctcactg
    27721 ctctgaaact ttatggaccc tgctctatcc atagaaaaac tttctctccc gtctcggaaa
    27781 tgatggaatc tcagattaaa taaacaaact ttaatatctt cactttcaaa tatatattct
    27841 tatttaaact tcaatttttc tctatctata taaatattat tgattaggaa tagaagccta
    27901 aaaaaataca agtccatttt attaggtttc aaattaataa atacatccaa gttatatgct
    27961 attaactcaa tatctatatt atccttgact taattaaagc tcttctgata aaaggattat
    28021 taatctttag gtttgaattt acaatataaa atactgataa aggagcttca ttaatggaaa
    28081 aaatgatgct attgggtgat gaagctgtag ctcaaggagc tctggatgcc ggaataagtg
    28141 gcttttatgg ctatccagga actcccagca cagaaatcat tgaatatgcc caacaaagta
    28201 aacaagcaga agaaatgaaa gtccatcgga attggtcatc taatgaaaaa accgcattag
    28261 aagctgctct gggtatgagt ttttccggga aaagagcaat ggtaacgatg aagcatgttg
    28321 gattaaatgt ggcagcagat ccctttatga attctgcctg tacaggtgct catggtggtt
    28381 taattgtagc agtggcagat gatccgagta tgcattcttc tcaaaatgag caggattcac
    28441 gcttctacgg aaagtttgct atgattcccg ttctggaacc ttccaatcaa caggaatgtt
    28501 atgatatggc attttttggc tttgaactct cggaaaaata ccatattcca gtcctgttaa
    28561 ggctaactac ccgtctggct cattctcgtt ctggtgtaat aagaagaaac tccattcccc
    28621 agaaagaatt aaaattacca gaagacttgt atcaatttct actgcttcct gctatcgctc
    28681 gtagaagata caaacatttg attgagctgc aatcagattt tctgaaagaa gcagaagaat
    28741 ctcccttcaa tcaattgact ttggatgcaa aagattactc tgtgggaata attacaactg
    28801 gtttagctta taactatctg cgagagaatt ttatagacca gcctatcccc tatccagttt
    28861 taaaaatatc gcattatcca ctacctgaaa aggctattca tcaattgtat gataagtgcg
    28921 actctttgct ggttctggaa gaaggaatgc cttttgcaga agatatttta aaaggacttt
    28981 cctggacggg aacaaaaccc atacatggta gattagatgg cactattgaa agagatggag
    29041 aattaaatcc taataaggta gcaagagctc ttggacttcc tgatacagaa ggtaagccag
    29101 taccatcagt ggttcgtcca agacctccag ctttatgtgt gggctgtcct cattccgata
    29161 gctttctggc tttgaatgaa gcccttaaag attatgggaa aggtcatgtt ttcagcgata
    29221 taggatgtta cactctcgga tttatgcctc cttataatgc tattaattcc tgtgtggata
    29281 tgggtgcaag cataactatg gcaaaaggag ctgctgatgc aggactctat ccttccattg
    29341 gagttattgg tgattccacc ttcttccatt ctggattaac tggattaatg gattgtgtct
    29401 atgaaaatgc caatgtggtt attgttatcc tggataattc cactactgct atgactggtg
    29461 gtcaggaata cactggttat ggaaagattg aagatgtatg caaaggtatt ggagttcatc
    29521 ctgaccatat ccgcatcttc aaacctctga aaactaactg ggatgaaatg gtgaaaattt
    29581 atcaggaaga aatagcttat aatggtgtat ccgtggttat tccacgccgg gaatgtattc
    29641 agactttaag aaaaaagaat aaaatggaga ctccagaatg aaaaaagata taatactcgc
    29701 aggtgttgga ggtcagggaa tattgactat agctacagtt cttggagctg cagcacttaa
    29761 tagaggctgg aatctaaaac aggcagaagt gcatggtatg agtcaacgcg gaggagatgt
    29821 gcaatctcat cttcgtcttt ccgatcagat tatctggagt gaccttattc cttatggcaa
    29881 agcggatatg attcttgcgg ttgaacctat ggaggctttg cgctatctgc cctatttagc
    29941 accagaaggc tggattattt ctaataaaac tcccttcaag aatattcctg attatcctga
    30001 tgaagaagag ctttatgctg aaatcagaaa aatacccaat aatgtgctca ttgacgcaga
    30061 ttctattgct aaatctgtaa agtcacctcg ttctatgaat attgttatgc ttggagcagc
    30121 aataaaatat ctggaattta caccagagga actgaaagaa tccataaaaa tgatattcaa
    30181 cagaaaggga gaaaggatag tagaagaaaa tcttagagct ctggacgcag gtattaatgc
    30241 ataaataatt tataaggagc atcgtaactg gtgctcctct tatttttttt aggaggatac
    30301 aatgaaaaaa agaaaagggc tgagtaatcg ccttttcaga ttctggttct tttttctctc
    30361 tctaaatata tcctctttgc ctgcagaaat ccgctttcaa accatagaag actttgaaag
    30421 tggttatgtg aatctgctct cttatagcaa tgaagaccag aatccaactg cctgggaatt
    30481 aacctcttcc gatacttata ataatagtgc ctgggcttta agattatatg gaaatacctg
    30541 gaagctacaa atgataaatc ccgtggtagt tgattcggga gccgtttttc aggttgccgc
    30601 taaaacttct acaggtgcaa aaatccaggg tgtaggcttt tccgatggaa ataatgtcct
    30661 tttctattcc tttgcgggaa cacaaacctt aaatattgaa gaatgggtaa ctgtctatca
    30721 gggagcatta aatgttttgg aatggaaaag attttaaaga aaagagagtc tcatctggag
    30781 tgtattttat tagactggaa cagggtggaa acaccacggt gaaaaaagta ttacttttac
    30841 attgaagcct ctataattga caaatactat aaagcttaat tgatggctat ttatggatga
    30901 attaatagtt ttcagtgtct cggaagtaac ttcgcatttg aagcaagtga ttgaatctca
    30961 gattgaaccg ctttatgtgc gtggcgaaat aagtaattac actcgtcatc cttccggtca
    31021 tatatatttt agcttaaaag atgcaaatgc cactttgcgt tgcactttct ttcgtggttc
    31081 caatatctat cttgattttg aacctgaaga aggtatggaa gtgatatgct atggtaagct
    31141 caccatttat gaaaaaggca gcacttacaa tcttaatgtt tactcaatgc aactggcagg
    31201 aaagggagat ttagccagac gatttgagct tttaaagaag aagttacaag cggaaggtct
    31261 atttgaagaa gcacataaaa agcccttacc aaaattccct cgtcgtatag gaattgtcac
    31321 ttctcccgca ggagctgctt tacaggacat cttaaatatt ttaagacgcc gttatcctgt
    31381 ggaagttatt gtatttcccg ctttagttca aggtgaagaa gctcctccca ctttaattca
    31441 aggtataaga tactttaatg aaaaagagaa tgttgatgta ataattcttg ctcggggagg
    31501 gggttctcaa gaagagcttt tctgcttcaa tgatgaacaa ttagccagag ctatctatga
    31561 ttccaaaatt ccgattatat ccgctgtagg tcatgaaata gattttacta ttgcggattt
    31621 tgtcgcagat ttaagagctc ccactccttc tgcagcagct gaattagtgg tccctaatag
    31681 agttgatata attaactctc tcaaagcttt agaagaaaaa ctaaccaata gcatcaaaaa
    31741 taagatatat attacccgca atcagattaa tgaattgaga ttagctttaa cttctcttca
    31801 tcccagagcc atttggcaaa actataaaaa aagaattgac caggcttcta tagaatttct
    31861 aaatttagcg tccctaatgc aacaacaact ccataaaaac agtcagcagc aaagtgaagc
    31921 ttttcacact atgcaatatc tcttacaaag taaactgcat caatatcagc aaaatctgga
    31981 tgaaatgacc ttgactcttc cctctgagat ggcaaagaaa ttcaaggaaa cggaaaatag
    32041 aatttcaatg cttgagcttc agttggaaag tgtatcacca aaacatatgc tggaaaaggg
    32101 atatgctctg gtacaaaaag gtgaacatat aattacttct gtacaagaac taactcttga
    32161 agaagagctc tctttgagaa tgaaggatgg ctcagctgag gttaaagtgc aggatattta
    32221 tccggaactg gataatggta attccagttt agaacagtag attatgaatt tgaattcctt
    32281 ttctgctaaa aacgaaagct tagtatgacc gaagaccgca gtaaacaagc aggaattcta
    32341 actacagccg aatttattcg ttttggcttc aaaaccatta ttggcattgt tttagctcgc
    32401 atccttcttc ctgtagagct tggttcttat cgtcagcttt ttctaattta ttccaccttt
    32461 tccacacttt tactgcttgg tatccctcaa agcgttcttt attttcttcc caagctggaa
    32521 catatagaca gcaaaaaaga gtttataact cgcactgtca atcttatcac tctgctggct
    32581 tttgtatttg ggctggcaat ttttctgctt agaggcttta ttgctcatat attcaataat
    32641 cctcagctga atatactact tattatcttt gctgtctatc ccctatttat gttcatcact
    32701 caaatctact cccagattat gctgggattg aagcaaccca aaaagactgc tactttcact
    32761 ttaatcagtg tcgcttgtga ttttattttc attttaggaa ccgctttaat caccagaaat
    32821 cttcattata ttgtcctggg agtgatgttt tctgctttgc tgcaatgggg ttatgctcaa
    32881 tataatttgg gaagatattt cactaaggtt acctttgatc ccgattttta tagagaaata
    32941 tttaagtatt ctttacctct tggcctggct tcaattatag gaatgctttc tgttcagctg
    33001 gataaatttg tcgtttccgg attttttact cctgctgcgt atgctatctt ttctattggt
    33061 gcaattgaac ttcctctaat ttctattctt aataattccg tgaatgctat cctgcttcct
    33121 catattagta gtgctccaga tcagatatat actatttata gagcttcagt gagaaagaat
    33181 gcgcttatta ttttccctct ttgtgcttta ggtcttattt ttgctgaacc attaataact
    33241 ttgctttatt ccagcactta taaagcctca gctcctattt ttcaagtgta tctcattaat
    33301 ttaccgcttc ggatagccac ttatgggatt atctttatgg ctttgaagaa gactaagtat
    33361 ataatgatta attctatcat aaccctgagt atgaatctgg tgctaaacat tattttagtt
    33421 aagttaatag gaatgattgg agctgcgatc tctacagtaa ttgtaagctg gctttcagta
    33481 gccatatatc tttactggat aaaaaacagt ctaaaactca gtctaactga acttttcccc
    33541 ttaccggcaa taggtaaaac ggctttagct gtcaccattt cagctttaat aagtctttct
    33601 gtactctgga tattcgggaa gagctggatt tttcagatag tagctttagt agtttttata
    33661 atagcctatt ttttaagtgg caaagcatta aatgctattc ttccctatga cattcagtat
    33721 ataaaacagc ttttaaatca agcaaaattg aagctatttg gaactaaata gatggattac
    33781 tactctgaac atacctggaa acattatgat agtgaaggtc gttctttaga acgtttagct
    33841 tgtatcaaga aaatgattcc tgaagatgtg aaaactatcc tggatgcagg atgtggcaat
    33901 ggtattatta ccaatgaatt agccagagat tatgaagtta cagctttgga cccttcagct
    33961 atcgctttgc aatatgtgca atgtgccaaa atctgtgctt ccattgatgc tattcctttt
    34021 cctgataact cttttgatct ggtatgcagt aatgaagtgc tggaacattt agatgatatt
    34081 actttgcaaa aaggcattca agaaattaag agagtaagca aaaagtattt aattattgga
    34141 gtgccttata aagaacagct ggaaaagaag ttctatcgtt gtgctgtatg cggtttctct
    34201 gagaatgttt acagccatct tcaatctttt gacctatcaa aactggatat actcctaacc
    34261 ccggaatttt accggataga atatcacatt tttggaccaa tggaacgcaa tattcatccg
    34321 tttcttttat ccattttgca taagcaggga cagtggtttc tgccttccga aggtcaaaaa
    34381 tgtcctcaat gtggaagcac ggaatttctg cataagagca atcttttaac cagaatggtt
    34441 aacggaatga acctgatttt ttctcatcct catccttact ggctttatac tctttatcag
    34501 aaaatagata atatataatg aaaacagtgc tttatatcgt cttttattac ccaccagtag
    34561 gtggttctgg agttcataga ggagtaaaat tcactcgtta tctgcctgaa tttggctggg
    34621 acagcattgt tttaacacct aatcctaaac tgcttaagca acctaaagat tatagtcttt
    34681 taaaagaatt atccccagaa cagaagatag aacatttctt tactttggat gctcgctggt
    34741 tatttaagat tttctggggc ttgaatttac ctcaagtagt aaactggcta aattatcata
    34801 tattcaaacc tgatgaagat atcatcagtt tgccttttct acatcgcaag atatcaagcc
    34861 taatgaaacg gcaaaaaata gacctggtct tcatttctgg tcctcctttt tctctaatgt
    34921 taaatgtctt atggctcaag aaaaattacc ctctgcctgt tattacagat tttagagatg
    34981 actggagtca gggacaaagt cgcttggata atcctcctcc agaatctttt aaacagtatg
    35041 aactccagtt ggaacatcag gtcctggcta aatctgataa agttatagta gccaatgaag
    35101 ccattaagga taatttcctt tccattcatt ctgagatgag tccagaacac tttgtcgtta
    35161 tcaccaacgg ttatgatgag aaggatttcc agaaagattt tccacctcgt gagacagaaa
    35221 aagataagct tcagattgtt tatataggaa gtttatatgg acgtcgtcaa cctgcaatgg
    35281 tatggaaggt tttaattgat ttagttgaac aaggaaaaat caatcccgag aagataagca
    35341 ttcatatata tggtcctaac tatcgctctt ttgtttttaa aggctttgaa aataaccaaa
    35401 tccttgagaa gatagtaaaa cttcacccct atcttaatca ttctcaagtg ccggaagtga
    35461 tgagtcaagc agatgttctc tggttattta ttggtgcagg accaaaaagt gatgctattt
    35521 caaccggaaa aatatttgag tatatgagaa gtggaaaacc tatttgggca gttattaatc
    35581 cgcaaggtat ggcagcaaat atccttagac caagtgggtt atcttttatt gcaaataatg
    35641 aaaatcctgt tgatatttct aactcattag tgaatttata taacctatgg caagaagaaa
    35701 aacttgctgt tatgccaaat tgggattata ttcatagctt tgaacgtcgt gagttaactc
    35761 gtaaacttgc cggagtcttt gatcagactt taagtaaaat taatccagat aaaatgaata
    35821 acttagatta aattgaatta gcgtaatact atgaaaaaca aagtgctttt actacttccc
    35881 acgcattctt cttttatgaa agtagatgaa gaaatactaa aaagtgctta tgaagttaag
    35941 actatctatt tgcaacaaga tgtatccaga aaaagatatt ttcaaatgat agttagggca
    36001 cttttaactc ttatttttga ccatagctat caaaaagtgc tcatctggtt tgcagattat
    36061 cattcagcgc ctcttgtttt gttatctaaa tggaaaaaaa tccccagttt tatctttatc
    36121 gggggttatg atgctgtaaa gtatcccgaa ctgggaatgg gagtttattg ttccagagtt
    36181 cgtggatggt gtgccaaaat cgctttaaaa aactgtaccc atattatttc caatcatatc
    36241 gctcttcttt cttcaaataa cacttattat aaacctgaag gacataaaga tggcatctat
    36301 aatttaatcc ctggattgaa aactcctgct tctgtcattt ttaatgcagt atctatttct
    36361 cctcctgcaa ttatatccga acagagactt agacagatgg ttactgcagg ttctacacct
    36421 cgttttcagg atttttataa taaaggtttt gatttattaa ctgatatagc tcaaagaaga
    36481 ccagatatga gttttgtctt tgtaggaatt gataaaaaat ggttgcctga attgaataaa
    36541 atctatcact ttgaaaattt acccaatatc actatctatc catatttacc tcaaaaggag
    36601 ttcattagcc ttttagaact aagttctgtt tatgctcaac cttctatatc cgaaggaatg
    36661 cccaattctc ttatggaagc tatgctagcg ggttgcattc ctgtaggttc aaatgtggca
    36721 ggaattccca ctatcataga taaatatggc tatatcatag accatagaga ctctcaagaa
    36781 ctggaaaaag cattagacct tgcacttgaa actgatgctg atagaaagga aatatc
//
LOCUS       DAORZF010000011        33008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_1106_length_33008_cov_407.438382, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000011 DAORZF010000000
VERSION     DAORZF010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 33008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33008
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1106_length_33008_cov_407.438382"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(468..4394)
                     /locus_tag="PK015_02555"
     CDS             complement(468..4394)
                     /locus_tag="PK015_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOL33388.1"
                     /translation="MDFYWKNSLFECLYYPDELGFTSGTITSLAFYNQFADSPANGAT
                     KIWLGSTNLADLSAGWIPSTQLTLVFDGIVTYPAGTNTITIPLQTPYMHTPGNLVMMV
                     QRPMDTQYYSSSDYFLAQTVGSNRARKVQSDSTTYDPANPPASATVSGQFPKTTIFYT
                     GQAIVNDLGCLSISGPTNTSVGAVTPYIIQVKNNGTATQTNYTVKLMKEGGILLNSVA
                     GTAIASLQTLDFTIPWTPTESGTTYIYGEVSLTGDEIAANNQSSNLNVLVYPQGTIGV
                     TIGTGTSTGRMPVDMYYKNSLFETVYLASEINVGGLLTDIQFYNNFVTNLPNMPTNIW
                     IGETTQADLAAGWIPSTQLTSVFSGNVDYPSGQNDIIIHLTTPYPYGGGNLVVMVERP
                     MDTQYYSSSDLFRTQTGTVANRTRNVFSDTVDYDPANPPTATPTATFPKTTLMFITSG
                     LGSITGTVYGPGNIPLEGATVVVLGTTLSCTTSSTGTFTFPYVQAGTYQVKASKFGYN
                     DVTNTVNVVENETVTTNFVLTPLPQVTVSGRIVGSDQPTVGLADASINLTGYANYQAT
                     TNASGQFTINNVYANHTYNYLASCSGYQNATGTVDVGSTNVNMGDIILNELAYPPTQV
                     VATEIPTQNHVSITWHSPSSTNISGVDDFELNDGGWVPSSNWSNPLGDWEWTNTYDVA
                     NWSPEYTGTNVIPPPTCYSGTGMWGTKIYTNYTNSGGFNYLTKTFNLVGILNPELRFW
                     SWENVFGDFDFCQVAVNGTVIWGPSWDYTNTQWQERIIDLSSYAGMSEVEIQFQMYAT
                     TVVNYAGWYIDDVYVGPAQGKVINSPVCSVPFEMRGLSEREAAAFAEKEASNRARMRE
                     SGISRIEMTRSLVQDRIILGYKVWRLLQGQENNESSWILLTDPAIQDTTFIDTGWSNL
                     PDGTYKWAVKSIYSNNVQSEAAFSNTLIKVTYIGTIAGVVRNTSNAPIAGAIVSCGNV
                     TAITNTSGAYSMLVQTGTHTVSVFASGYLPAIQENVVVVTGQTTTVNFVLNESILIFQ
                     DGFESYSNFALSFAPWTLIDVDQSNTVGIQNVTFPNSGSPMAYIIFVPSATTPPLTGA
                     NPHSGIKEAASFASINPPNNDWLISPLLHYPVELKFWARSYTNQYGLERFKVGVSTSG
                     TNPSDFIIISGDNPVEAPVAWTEYTYSLTNYMNSDVYLGIQCVSDDAFIFLVDDVMVR
                     GVTTVDDENIPVLATELFNNYPNPFNPETTIRYSVKETSPVTIEVYNLKGQLVRTLVN
                     EVKTAGNYSVVWNGRDNNNQPVSSGVYFYKMNTGKYSSTKKMILMK"
     gene            complement(4519..5442)
                     /locus_tag="PK015_02560"
     CDS             complement(4519..5442)
                     /locus_tag="PK015_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33389.1"
                     /translation="MKRFILILLMGVIIGFSFAGIDEYYTFNATNGTYTPITGTNAGI
                     SSDDAISSAIPIEFTFPYGENSYTEIKISSNGWIGLGTSQTSSNLSNNLTSTTIVPII
                     APLWDDCSLAAGTCEYLLSGTAPNRLFTIQYSNLKWNYLSTTLFNLQVRLYESGKIDF
                     VYGSASGTPNIPSASIGINMLPGGSGWFYSVTPGTPATASTTTANNAVNVWPGEGTVY
                     EFNPVVAVPNDLAALSVTGNTTPTAGQSYDYIVTVRNRGSNPQSTYQVKLLLDTQEVG
                     SVNGTTIQPGEILTYTIPLDTHYSRSWSPLW"
     gene            6123..7391
                     /locus_tag="PK015_02565"
     CDS             6123..7391
                     /locus_tag="PK015_02565"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOL33390.1"
                     /translation="MSSITVLGCMWGDEGKAKIVDFIGAEADVVARFQGGANAGHTIV
                     LEDKKYIFHTVPSGILYPETKCVIGAGTVIDPFGLRDEMESLIAQGIDFTNRLFIDDR
                     AGLVLSLHRQLDQKSEENLGAGKIGTTGKGIGPAYSDQAARCALRIGDLRYPEWLEQR
                     LDNFYKYHQITLSVSERKEELESLLEVGKFFQPYIAQTDQMLRNWYREGKSILFEGAQ
                     GTLLDLIYGTYPFVTSSHTVSGGVCIGTGFPPRWLDNILGVFKAYTTRVGAGPFPTEI
                     EGPLADRIRQKGNEFGSTTGRPRRIGWFDAVAAQYSASLNGIDSLAITLLDVLTGLPE
                     LKICTSYLKHNRKLPGFVTHPIELKEIQPEYLTLPGWDIDISNIRKWDTLPKEAKQYL
                     EAIQDLLKLPVKIVSVGQDRRQTIVNKKKV"
     gene            7393..8583
                     /locus_tag="PK015_02570"
     CDS             7393..8583
                     /locus_tag="PK015_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33391.1"
                     /translation="MKKGCLLLLLLGIGCIFAQPLEFPQPQFITPIFDSFTRNYVSTS
                     AMGRGYTGITIPGGMDNALLNPAAYLPDKPALHLELLAKPSIKVDFYAAQDTSTGYNM
                     TANDRLTSPVPFGIFAVGSSVKDHISYGFLYSMPKTVRMEDFSVATNMGMSLIVRYPT
                     FNLHQFTANAAYHKDQFHFGVNLHNQFYYLGDVTFLRTFDRVRDTKYLLRPQFGFLYT
                     GENINTGLTLVPPQKTNWDLKYVIYDTKLPLNVSGGVAYKHNGTQLAAELEYEQCSAI
                     CDEFKDRYTFHLGAEKTIEQYTYRLGYIYHPEVWHGDYLLPDAASDTISIWWDKILPG
                     GTVKKNTQHILTGGFSWHHKDVNVNLSGLVNVANTAPVSQISLSVDLYFSAFQRKDFL
                     YFGK"
     gene            8580..9701
                     /locus_tag="PK015_02575"
     CDS             8580..9701
                     /locus_tag="PK015_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOL33392.1"
                     /translation="MTKVQIRRLDDYNLPPLQEAVDEFLNQEFNSHWKHSKKVLLKPN
                     LLGAFPPERAVTTHPALLEALILYFLDLGKEVWIGDSPGGTANVEKVWQTCGLQDLAE
                     RYPVRLLNLSTTGYRELIYEDISVKISDILWNFGIIINIGKLKTHSLVGFTGALKNLF
                     GLVPGLAKSDYHRLYPNTNDFCRLLVAIYQLTKEYISYSFIDGILGMDGPGPSAGRVR
                     KFGLLLGSKSISSLDYVASKLMGFKLKDVPYLWDALHKDGQLISDIQIPTSFQDFKLP
                     DVDIRTVKMSNKFLRYVPRIVRYPFQRLYDRRPIITSRCQKCSICVKSCPAKAISPPD
                     KINFPVIDRKKCIKCLCCHEMCPYEAIDIHKTLLAKMVG"
     gene            9701..10081
                     /locus_tag="PK015_02580"
     CDS             9701..10081
                     /locus_tag="PK015_02580"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOL33393.1"
                     /translation="MKKQSNKNRRFLNFIYVLLILLLLSWILFWGNNSFYKKWKLKRK
                     INRMEQQYNALKAQNDSLANYNYRLKTDPEAAEEVAREEHGLIKSDETVFIFKEATND
                     SLKQEQQSGKKSSKFPVTKTIIKK"
     gene            10086..11735
                     /locus_tag="PK015_02585"
     CDS             10086..11735
                     /locus_tag="PK015_02585"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOL33394.1"
                     /translation="MANQKVPLFSAQEERELRKQLSLLLYCSQASQSQNIINQLLHFN
                     VPDKSKKELSLTRFRYFSAALIEQLIILLQDEQFLKPSRWTDFFELLNYFPLSSENPI
                     PHHLKETISSSTNNNPFYQSDNFIPLQLSYLLAQLEDSDTAYSLLKQLKEDISITTPF
                     PHLIYNLTLARLLILKNKRLDFTELWLKLIYKYYQEDGSETALHLILQWQKMIPWGRN
                     ASLKKKVLQTYSSDWKYNKTLLSAFILNELVTLENHLIKPEEKIRYVHILLSLPSSFL
                     SFEQLQYLYFFAGNYYSGVEQNFPKSIRSYHHSTYYLHKSWLALQNLSQFLRENLSPE
                     QMVQIMPYLEKKVISLANQINLQNSTYVDTLHKNYLLISDLYHQVEALSITDHLTGLK
                     NRHYLHNNLYHTFQLAARHKVPISFAILDIDHFKQVNDIYGHLAGDYVLQTLAELLLS
                     FFRKSDVIIRYGGEEFLLVLFDIKKENMLKLMEEFRAKIENYEFIYQDNKFHITISTG
                     CVCNCFACLNDTALNSYIEKADKALYTAKEAGRNTVVCFTE"
     gene            11792..12895
                     /gene="ugpC"
                     /locus_tag="PK015_02590"
     CDS             11792..12895
                     /gene="ugpC"
                     /locus_tag="PK015_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HOL33395.1"
                     /translation="MARVLVQNLNKIYDNNVHAVKDVSFEAEDKEFLVLVGPSGCGKS
                     TILRMIAGLEEITSGNIWIGDKLVNDVPPKDRDIAMVFQNYALYPHMTVFDNMAFALK
                     LRGEDKQVIKKKVQHAAELLGIQDLLKRKPGQLSGGQRQRVALGRAIVRNPKVFLFDE
                     PLSNLDAKLRVAMRSEIVKLHKQLANTMIYVTHDQVEAMTMANRIAVMKDGIIQQIDT
                     PLNIYNHPANIFVAGFIGSPAINMVEGRVESVDSNLFFTSEDFKVQIYPEHQKLLISY
                     VNKSIIMGIRPEDIYDARYDSMAEFPQRIETICDLVEPLGSEYHVVLATPHHNFIARL
                     EPKELPKMNEKLTISLNMFKAHYFDPETEQCLV"
     gene            12929..13687
                     /locus_tag="PK015_02595"
     CDS             12929..13687
                     /locus_tag="PK015_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33396.1"
                     /translation="MHLKVGSFLVILLLFPTLNAIITLDTASTNALSGITILSNSIAD
                     FALSPNISNTGIAFSAHIPFGMTDCTVYCLNTATQFNPFILSTGIAHLDQGDYRNQDY
                     FLGLAFHYKNLTLGYTEHLQYEKISTGNSYHTWLSNVALTYSCPEYGAEIKSLHLTSS
                     DEEWHISAMVKIIDEVYGAGSYVYIPNYEDNYRIATSIKLNEPLSLLVSWQNTPSQFG
                     MGIKLKIKRGELTYAIRTHSELNLSHSLDLGFNW"
     gene            13684..15195
                     /locus_tag="PK015_02600"
     CDS             13684..15195
                     /locus_tag="PK015_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33397.1"
                     /translation="MKSFVVISFLLSLTFLLYAQYYDELIELDTPALVDSAWNNYYQE
                     LLELEKPLNISTRLYLQNTNNYCLNSLQYEIPQGILLLNNRYNIDTGNNDWGLHLNLK
                     TERVNLVLGSYRVRFGRGLVCGTGSRKLPDSPFKLYMPTSPSTYTPLGAAFIYQGNSL
                     QTLVFASVQNRVARLKDERITSLPTTNSDNLSHTQENIYGTSVAWVNNSFQIAGLLYR
                     QQYDRAFNDSLLVSNLNAYSLYAALKLPNNQISTELSYANHQLSTILSWNYHLKSFSQ
                     TLSYAQNTIYHQFPYALSASILNHNTDRKEINCDLKWNVLTNTNLQIHYSINIGPAFS
                     GEPFSRFISSLAYDKRGTRWKLNFYQYDRELITWIDSTYISTIPEHYRIQTTGHLPIT
                     TYLFQQFEFSYTLQNKEDYTKNTYHFQYFLGYKKSIWEMRLGYTSWQSPHNFYIADEI
                     SPEYYSMCSSEETAISITTGLKFKEAAFSLYGEKSLLSSKKYRIRIQLKVSIF"
     gene            complement(15201..17420)
                     /gene="topA"
                     /locus_tag="PK015_02605"
     CDS             complement(15201..17420)
                     /gene="topA"
                     /locus_tag="PK015_02605"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOL33398.1"
                     /translation="MPKNLIIVESPAKANTISKFLEHKYDVKASMGHIRDLPENELGV
                     DIAHNFRPVYRIDKKKSKVINELKEAAKDADTIYLASDHDREGEAIAWHLTKVLEKEL
                     GGKEVFRIVFNEITSRAIQDAIKNPSSIDMAKVDAQQARRVLDRIVGYTVSPLLWKVI
                     AKKLSAGRVQSVALRLICEREEEIQNFVPKEYWKLEAKFHRDNLPPFKAALEKWENKK
                     IDIPDEATAQKLIEEIDTSPAVISDLKRSTHKLEPLPPFITSTLQQEASKILGFSSEK
                     TMKIAQELYEGVDLKGERTGLITYMRTDSLRIADEAIENCRKLIKERFGKEFINPAPR
                     VYKNKSTAQDAHEAIRPTNTFYTPESVAENLTKDQLKLYTLIWQRFVATQMKPAKLLR
                     TKVEVTVGKAIFSANGNVVEDEAFLKVYPYMKIISGEAIDSAYEKGDNLEYGKIESSQ
                     HFTTPPPRYTEASLIKELEAKGIGRPSTYATIISTIRKRRYVGMEKKNFFPTKLGIDV
                     NRFLVSNFDDIFNVGFTADMETKLDEVEENKVIWHELVKNYYEELMNLLNKVDVKKSK
                     QEFIEETDIVCDVCKEGKMVIRHSKNGEFLSCSRFPACKNTKKFIRDDKGNIVIVEPQ
                     TLEEKCPKCGSPLIERKGKYGEFIACSNYPKCKYSRNKTTGVTCPECGKGELIQRRNK
                     KGNIFYSCNRYPECKFISSYKPLPISCPECGNPYLVEKKTREGDSYKECPKCKSRME"
     gene            complement(17446..20610)
                     /gene="secA"
                     /locus_tag="PK015_02610"
     CDS             complement(17446..20610)
                     /gene="secA"
                     /locus_tag="PK015_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOL33399.1"
                     /translation="MLEKILRKMFGDKNTRDLNKYLPQVQLINQIYEGLAQYDDDQLR
                     ERVAEIKQEIAEKLSDLREKLEELEIRYREEEDEAERNRIDIQIDETKKALKNLTKST
                     LDDYLPEVYAIVKDTCRRLVGKTFMVRGHEVVWDMVPFDVQLIGAMVLHDGKIAEMAT
                     GEGKTLVATLPLFLNALVGRGAHLVTVNDYLAARDAEWMSPIFNFHNLTVGCITTDMD
                     FDSRKKAYQSDVTYGMNSEFGFDYLRDNMAVIPEQLVQRDFYYAIVDEVDSILIDEAR
                     TPLIISGPIAQDKNFYQELRPQINQLVQAQNTLVQKVLSEIREDLREDNPNSDIERLG
                     RNLLLVQRAAPRNKAFTKIMQDPKLKKLVNDVEGIYLRDKKLPELDDNLYYVVDEHQN
                     TVDLCEKGRDFLSKHDKNLFLVESLDEILNSIDEDESLTEEEKLKKKARETNRFMDKS
                     EKLHNINQLLKAYTLFEKDVEYVVVDNRVVIVDEFTGRLMPGRRYSDGLHQALEAKEN
                     VEIEAGTQTFATITLQNYFRKYERLAGMTGTAATEEAEFVEIYNLPVITIPTNVPVTR
                     IDHEDVIYMTKNEKYQALIDEVIYWHNLNKPVLVGTVSVEVSETISRMLKRRNIPHNV
                     LNARQHQREAEIIALAGQPGAVTIATNMAGRGTDIKLGPGVVTKSIEEYRGIDPSITP
                     EYPYGLPLDGLHVIGSERHESRRIDRQLRGRAGRQGDPGTSRFYLSLEDDLLRLFGSE
                     RIAPMMTRMGMKPGDAIRHPWMTKTVERAQKRVEENNFEIRRNLLKYDEVMNQQREVI
                     YSYRRSVLKGYSLKREVQEMIEETIDRIISENISFDSSPDEWNLARLTQWFKSALNVA
                     IEPEDLESDHLSEPLLRNTLKELILQAYEKKEEQVGSESMREIERRCLLEVVDEEWRD
                     HLHEMDLLKEGVYLRAYAEKDPLIEYKRESFKLFQNLISRIQENVTKKVFTTYILTQE
                     QVQNMMKDSKLQHENVSAFLKSQELRPGQQNMSEPPNFSEPDSGVVKQKPIRVAQHVG
                     RNDPCPCGSGKKYKKCCGRFENDE"
     gene            21170..24970
                     /locus_tag="PK015_02615"
     CDS             21170..24970
                     /locus_tag="PK015_02615"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOL33400.1"
                     /translation="MKKHLLIILILSLVTFVFAAQGSIKVSNTPTAAVVTNSNDYGLS
                     VSYSIEKLDFQEIQTSEGLFTDLWVADYAATNTIGLPKLPLLRQIFSVPEGSDVQASM
                     NSVLRKNIDLKAEGINNPIIPRQEPVSKSVDPATLPFIVNQEFYQSKNWTDFPSIQVT
                     DLGYMRGYHLYALDFTPVRYNPGTGEIEVIYSAEVNVQFLHPDLVSTNELRAKTYSPV
                     FDSMLKTILMNPDSNRASLNRYPMSYLIITPPNFVNALQPFVNWKTQEGYNVIVATTT
                     QTGTTTTSIKNYIQNIWDSATTENPAPTYLLIVGDVAQVPANSASASPGGHVTDLTYV
                     RLQGNDFIPEMYFGRFSATTEAEVTNQVNKTLMHEMYTMPSDAYLSEVVMIAGVDASW
                     SPTHANGQINYGVNNYFNAAHGINSHTYLYPASQNASSSIINDISNGVGYANYTAHGS
                     ETSWYSPSFTIANINSLQNVNEYPVVVGNCCLTNAFDTGVCFGEAWLRAENKGAVSYI
                     GGTNSTYWDEDYYWAVGYKPPIVGTGSPFVPGHTGAYDAMFHEHNEPFEDWANSVGAI
                     IFMGNMAVVQANSPRINYYWEIYSNMGDPSLIPYLGIPAQNSFVPPATLFLGLGSLDI
                     TADPYTYVAISMNNELHGVGLTDASGNLTLNFTPFTEPGTAQLVLTRSLRRPMIANIQ
                     VIPNVGPYVTVSQITVNDPNANAIAEAGETISMNLTFHNVGVQPAANLTATLSTTCPY
                     VTILDGTATLPDILPDQTISLEGLFSVLISIYIPDQTSVPFDIIVSDGTNTWTTTRSI
                     VVNAPKLQFGDPILFDPDGDGFFEPGENISIGITITNIGHMNTDSGTLIILVNNPFVT
                     LSSNYFVLPTMAAGVNIPINFTVTLAPDIPDGIIVPISLSISAGQININHCILLPIGV
                     YGDGFETGDFSVYPWVNNSPIPWTVVNDPANAHSGNYVAKSGAIGHSASTELSVTLNV
                     SSAGNIKFWRKVSSELNYDFLRFSIDGEEKGSWSGTLPWAQFSYPVTTGPHTFKWTYS
                     KDSSVSSGSDCAWIDDITFPTSASGEIAIIYVPQTEFSFLELHPNDQVSADIVINNVG
                     TVELTGTISYPSVMNLLYNGFPVENDHNFSIPVGEARIYTLTYTAPETAVNLDLELII
                     TSNDINNSPIIIPIHITTVPNNDPATIPVVTSLEGNYPNPFNPETAINFSLKESGKVR
                     INIYNLKGQLVRQLVDTELPAGRHQIVWNGKDNQGRSVSSGIYLYRMEAKGYTNTKKM
                     MLMK"
     gene            25067..28762
                     /locus_tag="PK015_02620"
     CDS             25067..28762
                     /locus_tag="PK015_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOL33401.1"
                     /translation="MKKILLIITLLLTLTFALTAREITVSSYDNHIQLLDSNPEHMVM
                     QLTLGHFNSEPVDIDGNTWNLISLKKEGLTLEAGYPQVPVLARSVIIPNTAKMELSIL
                     NSEYVEMILPVAPSKGNLTRDINPEMVPYYFADFYSGNEFWPENLSYLTEPFILRDYR
                     GITVRFQPFVYYPATQTLRIYTKLTVALEVNGTDLTNSLPVAKTSYAQEFAPLYENMF
                     LNFNQAKYPSLAEQGRILAIKNSMFDNVMQPWIDWKRQNGYRVDVVDVTVAGPTATQI
                     KTYIQNQYNLNDGLMFVQIFGDAPQVPTLTSGGGGSDPSYSLLAGSDSYPDIYVGRFS
                     AQTVAEMETQITRSVHYERDIQAGSQWIQRAIGIASNQGGGSQGDMGESDQQHQENIR
                     TDLLNYGYLSVDQIYESMGATAAQVSTNLNQGRGFINYTGHGADTYWVTTGFSNTHVN
                     ALVNDYKLPFIQSVACVNGNFVSQTCFAEAWLRSVNEATNAPAGAIGFYGSSINQSWN
                     PPMRAQDETVDLLVAEQKHTMGGLFYNGSSKMIEVYGQDGISMFLTWNIFGDCSLMVR
                     SKDPQPITATYTPILFIGMNTFPVNTIPGAKVTLYSAGTVYATGYADANGDINLTLEN
                     PPLEPMDLTLTISAFNKVTYLDTIQVLPASGPYLVVADMMVTDNNNNIPEYGELITLN
                     LMFSNVGNDIAINPTVSIQTEDPYLTIINGTENIPDIAANSIGGTVNGLTIQVASNVP
                     DQHTASIHIIATVADESYEYDRNLVLNAPAFTWEGLIIEDYLGNNNSVLDPGESVTIC
                     LPVTNTGHAQANNISTALAIVGGMNVIEPMDDSFSALAPGEQCQITYYITLSSQVAQG
                     TNLLLTAMLFAGDYSSVQNYPITVGLLMENFETNFSGFNWQFSNGNWTIDNTSYNSTH
                     SAKSATINHNQTTSMSVTLNCPVDGTISFWKKVSSELNYDYLRFSINNVVRSQWSGTS
                     DNWSQVSFDVQAGQNTFTWSYSKDSGISSGSDCAWIDDIIFPSSGTIIGTPVIAIDTM
                     VVDFGVVPIGQIAQRTFTVNNNGDAVLIGTVQVAPPFAVYQGNGYPGFAINIVVPAQS
                     YLGFNLNFTPVDTLTYNSTMQINTDDPVNPVIMVQLVGKGTVVSNDDPLLPAVTELKG
                     NFPNPFNPSTTIAYSVKNTAPVSIEIFNVKGQLVRTLVNEVKTAGNYKIVFDGLDNNH
                     RPLASGIYFCRMQAGDYRQTHKMLMLK"
     gene            complement(28858..30153)
                     /gene="glyA"
                     /locus_tag="PK015_02625"
     CDS             complement(28858..30153)
                     /gene="glyA"
                     /locus_tag="PK015_02625"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOL33402.1"
                     /translation="MKHIQEQDPELYAAMTGELNRERENLELIASENFTSMAVLEAQG
                     SVLTNKYAEGYPYRWSKKTGKINYNLYGRYYGGCEYIDEVERLAIERAKQLFGAEHAN
                     VQPHSGSQANMAAYFTLVKPGDTVMALELSHGGHLTHGHPLSFSGQFYNIVPYQVSKE
                     TEELDYDELARLAEEHKPKMILAGASAYPRKMDFAKFREIADLVGAKLMVDMAHIAGL
                     VAAGLHMNPVPYADVVTSTTHKTLRGPRAGLILCKEEFAKEIDSKVFPGVQGGPLMHV
                     IAGKAAAFNEALQPEFKVYQQHIIDNAQALATALIANGFSLVSGGTDTHLMLINLGSE
                     EEGGPSGKKMEEALDKAGITANKNTVPFETRSPFVASGIRLGTPAVTTRGMGIEEMKQ
                     IADFIQRVREHIEDEEYLTNMKSEVRALTARFPLYPELG"
     gene            complement(30150..30590)
                     /gene="rpiB"
                     /locus_tag="PK015_02630"
     CDS             complement(30150..30590)
                     /gene="rpiB"
                     /locus_tag="PK015_02630"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOL33403.1"
                     /translation="MIIAIASDHAGFELKGAIKSHFPEHEFQDYGTYSVDAMDYPDTA
                     LPAARAVAEGKAERGILICGTGIGMSIVANKIKGIRAAVCMNADLARLSRMHNDANVL
                     CLAGRFIAVHYALVIVNTWLTTSFEGGRHQIRVDKIKEIEGERQ"
     gene            complement(30587..31507)
                     /locus_tag="PK015_02635"
     CDS             complement(30587..31507)
                     /locus_tag="PK015_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HOL33404.1"
                     /translation="MKEPIRSLEELANYVRSTGKRTRIAVARAEDPNTIASIGKAVNE
                     GFVEGILVGSKPKIEELCKAEQINPAIFRVIDTRDEEEAVTESVRMVRDDEADVLMKG
                     LVGTDKFLKAVLDKEKGLLPEKAIMSYVCALQLPGYHKLLFISDTAVIPYPDINQKIA
                     MINYSVAMARRFGIEKPKVALISATEKVSENIPHTLEYAELCKMAERGQIKNCVLDGP
                     LDIFLACDPQASIIKGVSSPLAGDADVLIFPSLESANSFYKGLMMFAKGELAGLIQGT
                     IKPVIVMSRSESAASKFYCIALSCLMGGEI"
     gene            complement(31504..32418)
                     /locus_tag="PK015_02640"
     CDS             complement(31504..32418)
                     /locus_tag="PK015_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HOL33405.1"
                     /translation="MQVTKLEQILDLIKPLPRKRLVAAWGVDPHTICAVYQAVDMDLI
                     EGILVGDEKQIYQVCETEQIDPSRFKIVHSESDIEAAAKAVDLINAGEGDFLLKGLLS
                     TDRYMKAILHKEKGLMDKGAILSHVTVAEIPTYHKLLIFGDVAIIPLPDLNQKIAITN
                     YLIKIAHYLGIENPKVGIQAASEQVLPKIPSCSEGAMIAKMAQRGQIKGAIVEGPLGF
                     DLIVDKESAQIKKVQSEVCGDADCILFPNIEAGNTFYKSVVKLMKGELCAVVMGARVP
                     CVLTSRGDSEKSKLYSIALASLLSGEKQ"
BASE COUNT         9885 a         6492 c         5939 g        10692 t
ORIGIN      
        1 gataagtaat agtgtgcaga tgtcttcatc tgcaccaagc agtataacct ttacatttat
       61 ttgaaagaaa ttgaaggcgg gataacttta catgtggttt tataattaat tttataagtt
      121 aatcacgcac ttgtgagctt gcgataaagt gaaagtcatc aggacattat gcaaaggagg
      181 acctttgcat accataactt tttcatacca ttaccaattt atataacata ttgaagttaa
      241 ggatattccc caacttttat tataggctct gacaaattta aaatcttact aatatgatta
      301 tagatataga actaaaatct atactaagtt ataaataagc gctggcaagt ttaaattata
      361 ctcatacaat gatagaaata gacataagtc tataataaat aagccctgac aaaccgaaat
      421 attggtactc tgtcagggct tttacttttt ttgataaagc agttttttta tttcatcaga
      481 atcattttct tagtactgct gtatttacca gtattcatct tatagaaata gacaccactg
      541 gatacaggct gattgttatt atctcttcca ttccagacca cagaatagtt acctgctgtc
      601 ttgacttcat tcaccagggt gcgtaccagc tgtcccttga ggttgtagac ctcaatggtc
      661 acaggactgg tctctttcac actgtagcga atagtggtct ctggattgaa cggattagga
      721 tagttattaa acaattccgt tgccagcact ggaatattct catcatccac tgttgttacg
      781 cctctaacca tcacatcatc taccaggaaa ataaaggcat catcagagac acactgaatt
      841 cctaaataga catcactgtt catataattg gtaagagaat aggtgtattc tgtccaagct
      901 actggtgctt ctactgggtt gtctccacta ataataataa aatcagaagg atttgttcca
      961 ctcgtagaga ctcccacttt aaatctttct aatccatatt gattagtata ggagcgtgcc
     1021 cagaacttca attctacagg ataatgtaaa agaggactga ttaaccaatc attattaggt
     1081 gggttaatac tggcaaaaga agctgcttcc ttgatgccac tatgaggatt ggctccggtc
     1141 aaaggaggag tggtagcgga aggaacaaag ataatgtatg ccatcggaga accagaattg
     1201 ggaaaagtaa cattctgtat tcccaccgta ttgctctggt ctacatctat cagggtccag
     1261 ggtgcaaagc taagagcaaa attggaataa ctttcaaaac catcctggaa aattaatata
     1321 ctttcgttca atacaaaatt aaccgtagtt gtctgtccag ttactacaac tacattctcc
     1381 tgaatagcag gtagatatcc tgaagcaaaa acggatactg tatgagttcc ggtttgaact
     1441 aacatactgt aggctccact ggtattggta atagcagtta cattcccaca ggaaacaata
     1501 gctcctgcta tgggagcatt agaggtatta cgtactactc cagcaatagt gccaatataa
     1561 gttactttaa ttaaagtatt ggaaaaagct gcttcagatt ggacattatt gctatagata
     1621 gattttactg cccatttata agtaccatca ggtaagttag accagcctgt atcaataaat
     1681 gtagtatcct gaatagctgg atccgttaat aaaatccagg aactttcatt gttttcctgt
     1741 ccctgtaaga gacgccagac tttatagccc aatattatcc tatcctgcac caaagatcgc
     1801 gtcatctcaa ttctggatat tccactctcc cgcattcttg ctctatttga agcttctttt
     1861 tcagcgaatg ctgccgcttc cctttctgat agacctctca tttcaaaagg aacagaacaa
     1921 acaggcgaat ttattacctt cccttgagca ggaccgacat atacatcatc aatataccag
     1981 ccagcataat tgaccactgt tgtagcatac atttggaact ggatttccac ctcggacata
     2041 ccagcatagg aactgaggtc aataatccgc tcctgccatt gggtattggt gtaatcccag
     2101 gaaggacccc agattactgt cccattaacc gctacctgac agaaatcaaa atcaccaaag
     2161 acattctccc aactccagaa acgaagttca ggattgagaa taccaaccag attaaaggtc
     2221 ttagtgagat aattgaagcc accggaattg gtatagttgg tgtaaatttt agtgccccac
     2281 attcctgtcc cagaatagca agtggggggt ggaattacat tagttccggt atattccggt
     2341 gaccagttcg ctacatcata ggtatttgtc cattcccaat cacctaaagg attagaccaa
     2401 ttagaagaag gtacccatcc accatcattg agctcaaaat catccactcc agagatattt
     2461 gttgaagaag gactatgcca ggttatagaa acatggtttt gggttggaat ttctgtagcc
     2521 actacctggg ttggaggata agcaagttca ttaagaataa tatctcccat atttacatta
     2581 gttgaaccga catccaccgt tcccgttgca ttctgatatc cagaacaaga ggcaagataa
     2641 ttataagtgt gattcgcata tacattatta atagtaaact gaccagaagc attagtagta
     2701 gcttgataat tcgcataacc cgtaagatta atggatgcat cagcaagtcc taccgtaggt
     2761 tggtcacttc ctacaatcct tccactaact gttacctgag gcaatggagt gagcacaaag
     2821 ttagtggtca ccgtctcatt ttctactaca ttaacggtat tggtaacatc attataacca
     2881 aatttacttg ctttaacctg atacgttccc gcctggacat aaggaaatgt gaaagttccc
     2941 gtagaactgg tagtgcagga taaggtagtt ccaagaacca cgactgttgc tccttccagc
     3001 ggaatattac ctggtccgta aactgtaccc gtgatagatc ctaaacccga agtgataaac
     3061 atcaaagttg tcttgggaaa agtagctgtc ggtgtagctg taggaggatt ggcaggatca
     3121 taatctaccg tatcactaaa gacattgcga gtcctatttg cgactgttcc agtttgagtg
     3181 cgaaagagat ctgaggagct ataatattga gtatccatag gacgctcaac cattactacc
     3241 aggtttcctc caccataagg ataaggagta gtgagatgaa taattatatc attttgcccg
     3301 ctgggataat ccacattgcc actgaataca gaagtcaatt gtgttgaggg tatccaacca
     3361 gcagcaaggt cagcttgagt ggtctctcct atccagatat tagttggcat attaggtaga
     3421 ttagtcacaa agttattata aaattggata tcggtaagca aacctcctac attgatttct
     3481 gaagcaagat atacggtttc aaagagggag ttcttatagt acatatctac tggcatacga
     3541 ccggtgctgg ttcctgtgcc aattgttacg cctatcgtac cttgaggata caccagtaca
     3601 tttaaattag agctctgatt atttgcagct atttcatctc cagttaagga tacttcacca
     3661 taaatataag tagtaccaga ttcggtggga gtccagggaa tagtgaaatc cagagtctgt
     3721 agagaagcaa tagctgttcc tgctacagaa ttaagcagaa ttccgccttc tttcattaat
     3781 ttgaccgtat agttagtctg ggttgcggtt ccattattct ttacttggat gatatagggg
     3841 gtaactgcgc ccacactggt attggtagga ccactgatac tcaagcatcc taaatcattt
     3901 acaattgctt gtcccgtgta gaaaatggta gtcttaggga attgaccgga cacagttgca
     3961 cttgctggag gattggcagg atcataggtg gtactatcac tctgaacttt tcgtgcccta
     4021 ttagagccta ccgtctgagc taagaaataa tcggatgaag aataatactg agtatccatc
     4081 ggtctttgga ccatcatcac cagatttcca ggagtatgca tataaggagt ctgtaagggt
     4141 atagtgatgg tattagttcc tgctggataa gtaacaattc catcaaaaac cagggttaat
     4201 tgagtggatg gtatccaacc agcactcaaa tctgctaaat tagttgaacc taaccagatc
     4261 ttggtggctc cgtttgccgg agaatcagca aactgattgt agaaagctaa agaagttatg
     4321 gttccactgg taaaaccaag ttcatcagga taataaagac attcaaacag agagtttttc
     4381 cagtagaaat ccatcggcac tcgagctgat tgatctcctg cacctatggt tactgcttgt
     4441 actccagcag gctggacagc aacattaaga ggaggagttt gatcgttatt aggattttga
     4501 tctccggcta agactacttt accataaagg actccaggac cggctgtagt gggtgtccaa
     4561 gggaatagta taggttaaaa tttcaccagg ttgaatggta gttccattta ccgaaccaac
     4621 ttcctgagtg tctaataata atttaacttg ataagttgat tgagggttac taccacgatt
     4681 acgaacagtt acgatgtaat cataactctg acctgcagta ggagtggtgt ttccagtaac
     4741 agaaagagct gctaaatcat tcggaacagc tactacggga ttaaactcat aaacagttcc
     4801 ttcaccgggc catacattaa cagcattatt tgcagtagta gtagaagcag tagccggagt
     4861 acctggtgtc acactataga accagcccga acctccagga agcatattga ttccaatgga
     4921 ggcagaaggg atattgggtg ttccactggc tgatccataa acaaaatcaa tcttaccact
     4981 ttcatataat ctaacctgta aattgaacag agtagttgaa agataattcc atttcaagtt
     5041 agaatactga atggtaaaga ggcgattggg agctgtgccc gaaagcagat attcacaagt
     5101 acctgctgcc aggctgcagt catcccatag aggtgcaatg atgggaacaa tagtagtaga
     5161 agttaaatta ttactcaggt tactactcgt ctgagaagta cctaaaccaa tccagccatt
     5221 tgatgagatt ttaatctctg tatagctatt ttctccatag gggaaggtaa attcaatagg
     5281 aatagctgag gaaatagcat catcagagga aatacctgca tttgttcccg tgatgggagt
     5341 gtaggtccca tttgtggcat tgaaggtata gtattcatca atacccgcaa aagagaaacc
     5401 tatgattact cccatcaata aaattaaaat gaatcgcttc attatctttc tcctcttttg
     5461 tatatttaca atattattta aaagtttata ttcataaaac tgtcaattaa aatataccaa
     5521 ttattataga aatattaggt tgatgttgaa aatattccat cttcataaga cagaaagagt
     5581 ttcactcttt ttatgatgtt aatgttccag ctaatttgat ttccttagtg ggcaaagtct
     5641 tccctggctt gttaatttgc ctggcatact aatatctcta tcatactgat atgcttaggg
     5701 tgctcattct tatggtattc agatatttct ggggtataga tgtttatggt gtgcagatgt
     5761 cttcatctgc accaagcggt agcagctaaa agcatatcgg aaagaaattg aaggtgagat
     5821 aacttcaaaa tgtagtttta taaataattt cataagctaa tcacgcaact gtggtcttag
     5881 cgataaatca taatcttgag gacttgatgc aaaggaggac ctttgcatac cattagctta
     5941 gcataccatt acctttgtat accatcatct ttgaataaca ttacctttga ataccataaa
     6001 gggtaccttt atatactatt catttttcat tttaatcagc tttagataac gtaccagaca
     6061 agaaatagtt tgacagaata gaattataga aaaaaagaga agtattgata aaggagcagt
     6121 ttatgagttc tataaccgtt ctgggatgta tgtggggtga tgaaggaaag gcaaagatag
     6181 tagattttat tggtgcagaa gctgatgtgg ttgctcgttt tcaaggtgga gctaatgctg
     6241 ggcatactat tgtgcttgaa gataaaaaat atatattcca tacagtgcct tctgggattc
     6301 tttatcctga aacaaaatgt gtaattggcg caggaacggt gattgatcct tttgggttaa
     6361 gggatgaaat ggaatcacta atagcacaag gaatagattt tactaataga ttatttattg
     6421 atgaccgagc aggattggtt ttatctttac acagacaatt ggaccagaaa tctgaagaaa
     6481 atctgggagc agggaaaatt ggaactacgg gaaaaggaat tggacctgct tattctgacc
     6541 aggctgccag atgtgcatta cgaataggag acttacgcta tccagaatgg ttagagcaga
     6601 gactggataa cttttataaa taccatcaga taactctttc tgtttctgag cgcaaagaag
     6661 agttagaatc tcttctggaa gtgggaaagt tttttcaacc atatatagcc cagactgacc
     6721 aaatgctaag gaattggtat agagaaggga aaagcatact gtttgaagga gctcagggaa
     6781 ctcttctgga tttaatctat ggcacttatc cttttgtcac ctcttctcat accgtttcgg
     6841 gaggtgtttg tataggaact ggtttccctc ctcgctggtt agataatatt ttaggtgtat
     6901 ttaaagctta tactaccaga gttggagcag gacccttccc aactgaaata gaaggaccgc
     6961 tggcagatcg gattcggcaa aaaggaaatg aatttggttc aactacggga cgtccccgtc
     7021 gtattggttg gtttgatgct gtagctgctc aatatagtgc ctctttaaat ggaatagata
     7081 gtttagccat aactttattg gatgtattaa ctggtcttcc cgagttaaag atatgcacat
     7141 cctatcttaa acataatcga aaactacctg gctttgttac tcatccgata gaattaaaag
     7201 agattcaacc tgaatatctt acacttcctg gatgggatat agatatcagt aacatacgga
     7261 aatgggatac tttaccaaaa gaagcaaaac aatatcttga ggcaattcaa gacctgctta
     7321 aattacctgt aaaaattgtc tcagtcggac aggatcgtcg tcaaaccata gtcaataaaa
     7381 agaaggtata aaatgaaaaa aggttgctta ttacttttat tattggggat aggatgtata
     7441 tttgctcaac cattagagtt tccgcaaccc cagtttatca ctcctatctt tgatagtttc
     7501 acccgaaact atgtatctac cagcgctatg ggtcgtggct atactggaat aactatccct
     7561 ggagggatgg ataatgcact tttgaatcca gcagcctatc taccagataa acctgctctt
     7621 catctggaac tgttagccaa accctctatt aaagtagatt tttatgctgc tcaggatacc
     7681 agcacaggct ataatatgac tgctaatgat agattgacct cacctgttcc ttttggtatc
     7741 tttgctgtcg gaagttctgt gaaagaccat atcagttatg gatttctcta ttcaatgccc
     7801 aaaacggtca ggatggaaga ttttagtgta gcaacgaata tgggaatgag cttgattgtg
     7861 cgctatccta cttttaattt gcatcagttc actgctaatg ctgcttatca taaagaccaa
     7921 tttcactttg gagtaaatct acataatcag ttttactatc ttggagatgt gacctttctt
     7981 cgcacttttg atagggtcag ggacaccaaa tatcttctgc gccctcagtt tggctttctt
     8041 tatacaggag agaatataaa cactgggtta actctggttc ctcctcaaaa aactaactgg
     8101 gatcttaaat atgttatata cgacacaaaa cttcctttaa atgtttccgg aggagtagcc
     8161 tataagcata atggaactca gctggcagca gaactggaat atgagcaatg tagtgctata
     8221 tgtgatgaat ttaaggatcg ttatactttt cacttaggtg cagaaaagac gattgaacaa
     8281 tatacctatc gtctgggtta tatctatcat ccagaagtct ggcatggaga ttatttactt
     8341 cctgatgctg catcagatac gatttctatt tggtgggata aaattcttcc tggtggaacg
     8401 gtgaaaaaga atactcaaca tattctaact ggaggattta gctggcatca taaagatgtc
     8461 aatgtcaacc tttctggatt ggtgaatgta gctaacactg caccagtaag tcagataagc
     8521 ctctctgttg atttgtattt tagtgctttt caaaggaaag atttccttta ttttggtaaa
     8581 tgactaaggt tcaaattcgg cgtttagatg attataattt gcctcctctg caagaagcag
     8641 tggatgaatt tcttaatcaa gaatttaatt ctcactggaa acatagtaaa aaagttttat
     8701 taaaaccaaa tcttctgggc gcttttcctc ctgaaagagc tgtgaccact catcctgctt
     8761 tgttagaagc tctcattctc tattttctgg atttgggcaa agaagtatgg attggagata
     8821 gtccaggggg aacagctaat gtagaaaagg tgtggcagac ctgtggttta caggatttag
     8881 cggaacgtta tccagtaaga ctgctcaatc tttccactac aggctataga gaattaatct
     8941 atgaagatat atcggtcaaa atctctgata ttctctggaa ttttggaata atcatcaaca
     9001 ttggtaaact taaaactcat agtttagtcg gtttcactgg agctctgaaa aacctttttg
     9061 gattggtgcc cggtctagct aaaagtgatt atcatagact ctatcctaac accaatgatt
     9121 tttgtagatt gttagtagca atctatcaat taaccaaaga atacatatct tatagtttta
     9181 ttgatggtat tttgggaatg gatggaccag gaccttcagc tggaagagtt cgtaaatttg
     9241 gattgcttct gggttctaaa agtatatcct ctctggatta tgtagcttca aaactgatgg
     9301 gttttaagtt aaaggatgtg ccttatctct gggatgcttt acataaagat ggacagttaa
     9361 tctctgatat ccagattcct accagtttcc aggatttcaa attgccagat gtggatatcc
     9421 gaacggttaa aatgagcaat aagtttttgc gttatgtccc caggatagtt cgttatcctt
     9481 ttcaacgctt atatgaccgt cgtccgataa taacatcccg ttgtcaaaaa tgtagcattt
     9541 gcgtaaaaag ttgtcctgct aaagctatct ctcctccaga taagataaac tttcctgtta
     9601 tagatagaaa gaaatgcatc aaatgcctct gctgtcatga gatgtgtccc tatgaggcaa
     9661 tagatattca taagacttta ttagcaaaga tggtgggata atgaaaaaac aaagtaacaa
     9721 aaatagacgg ttcctcaatt tcatctatgt tctgcttatt ttattgttgt taagctggat
     9781 tcttttctgg ggaaataata gtttttataa aaaatggaag cttaaacgaa aaataaaccg
     9841 tatggaacaa cagtataacg cattaaaagc acaaaatgac agtcttgcta attataacta
     9901 ccgattaaag accgatccag aagcagcaga agaagtagca agagaagagc acggcttaat
     9961 aaaatctgat gagacagttt ttatcttcaa agaagctact aatgacagtc tcaagcaaga
    10021 acaacagtct ggtaaaaaat catctaagtt tcctgtaact aaaactatta taaaaaaata
    10081 gggttatggc taaccagaaa gttccactat tttcagcaca ggaggaaaga gagctaagaa
    10141 agcaactttc tttattactc tattgttcac aagccagtca atctcagaat ataattaatc
    10201 aactgcttca ttttaatgtt ccagataaat ctaaaaaaga actttcttta accagatttc
    10261 gctatttctc tgctgctctt atagaacagt taataatact tcttcaagat gaacaatttt
    10321 taaagccttc cagatggacg gatttttttg agcttttaaa ctattttcct ttatcttcag
    10381 agaatcctat ccctcatcat ctcaaagaga ccatatccag ttcaactaat aacaatcctt
    10441 tttatcaatc tgataacttt attcccttgc agctatctta tcttcttgct caattggaag
    10501 atagtgatac tgcttactca ctattaaaac aactaaagga agatatttct ataaccacac
    10561 cttttcccca tttaatttat aatctaactc ttgccagatt acttattctg aaaaacaaaa
    10621 gattggattt tactgagctc tggcttaagt taatctataa atactatcaa gaagatggaa
    10681 gcgaaactgc gcttcattta atcctccaat ggcaaaaaat gattccctgg gggagaaatg
    10741 cctctttaaa gaaaaaagtg ctgcagacct attcaagtga ttggaagtat aataaaactc
    10801 tgctttccgc atttatactg aatgaactgg tcactctgga gaatcatttg attaaaccag
    10861 aagaaaaaat aagatatgtt cacattttgc tgtctttacc ctcatccttt ttgtcttttg
    10921 aacagttgca atatctatat tttttcgccg ggaattatta ttcgggagtg gaacaaaact
    10981 tccctaaatc tatcagatca tatcaccatt ccacttacta tctacataag agctggctgg
    11041 ctctgcaaaa tttatctcaa tttttgcgag aaaatctttc cccagaacag atggtgcaaa
    11101 ttatgcccta tctggaaaaa aaggttatct ctctggctaa tcaaattaat ttacaaaata
    11161 gcacctatgt agatactttg cacaagaatt accttttgat aagtgacctg tatcatcagg
    11221 tggaagctct ttctataaca gaccatctca ctggtttgaa aaataggcac tatctgcata
    11281 ataatcttta tcatactttc caattagctg ctcgtcataa agtacctatt agttttgcca
    11341 tacttgatat tgaccatttt aaacaggtaa acgatattta tggacatctg gcaggagatt
    11401 atgtactcca aactctggct gagttattgt taagtttttt ccgaaaaagt gatgttatta
    11461 ttcgctatgg aggtgaggaa tttctcttag ttctatttga tataaagaag gagaatatgt
    11521 tgaaactgat ggaagaattt cgtgctaaaa tagagaatta tgaatttatc tatcaggata
    11581 ataagtttca tattactatc agtactggtt gcgtttgcaa ttgctttgct tgtttaaatg
    11641 acactgcctt aaattcctat atagaaaagg cggataaggc actttatacc gcaaaagaag
    11701 caggacggaa tacagtagtt tgtttcacag aataaaattc ctgcttgaca gcttatctta
    11761 tataaaatat tgatttattg aaggagtagt aatggcaaga gtacttgttc aaaatcttaa
    11821 taaaatatat gataataatg ttcatgccgt taaagatgta agttttgaag cggaagacaa
    11881 agaattttta gtgttagtgg gaccatccgg ttgcgggaaa agtactatct tgagaatgat
    11941 tgctggttta gaagaaatta ccagcggcaa tatctggata ggcgataaac tggtcaatga
    12001 tgtcccccct aaggatagag acattgctat ggtgtttcaa aattatgctc tttatcctca
    12061 tatgaccgtc tttgataata tggcttttgc cttaaagctt agaggtgaag ataaacaggt
    12121 tatcaaaaag aaggttcagc atgcagcgga gcttttaggt attcaggatt tgctgaaacg
    12181 caaaccaggt caactttcag ggggacagcg tcagcgtgta gctttaggac gtgctatcgt
    12241 ccgtaatccc aaggtttttc tctttgatga acctctttct aacctggatg caaagctcag
    12301 agtggcaatg cgttctgaaa ttgttaaact acataaacaa ctggctaata caatgattta
    12361 tgtgacacat gaccaggtgg aagcaatgac tatggctaat cgtatagcgg ttatgaaaga
    12421 tggcatcatc cagcagattg atacccctct taatatttac aaccatcctg ctaatatttt
    12481 tgttgctggt tttatcggtt ctcctgctat taatatggta gaaggtagag tggaaagtgt
    12541 tgattcaaat ctgttcttca caagtgagga ctttaaagtt cagatatatc ctgaacatca
    12601 aaaactgctt atctcctatg tcaataaatc cattataatg ggaattagac cagaagatat
    12661 atatgatgca cgctatgatt caatggcaga atttcctcag cgaatagaaa ccatctgtga
    12721 cttagtagaa cctctgggaa gtgaatatca tgtagttctg gctactcctc atcacaattt
    12781 catcgctcgg ttggaaccta aagaacttcc caaaatgaat gaaaagctaa ctattagctt
    12841 aaatatgttt aaagctcatt attttgaccc tgaaacggaa cagtgtctgg tttgaaaatg
    12901 ttctgattca tagcaataag aggcttttat gcatttaaag gtaggtagct ttctcgtcat
    12961 tcttttatta tttcccacct taaatgctat cataactctg gatactgctt ccacaaacgc
    13021 cttatctggc ataactatac tctcaaattc tatcgctgat tttgctcttt ctcccaatat
    13081 ttctaacact ggtatagctt tttctgcaca tattccattc ggtatgactg attgtacagt
    13141 atattgctta aatactgcca ctcagtttaa tccttttatt ttaagtactg gtattgccca
    13201 tctggatcag ggtgactatc gtaatcagga ttacttttta ggtctggctt ttcactacaa
    13261 gaatctaaca cttggttata ctgaacatct tcagtatgaa aaaatcagta ccggtaatag
    13321 ttatcacacc tggttgagta atgttgcgtt aacctattcc tgtcccgaat atggtgctga
    13381 aataaaatct ttacatttaa cttcatcgga tgaagaatgg catatctcag ctatggttaa
    13441 aatcatagat gaggtttatg gtgctggcag ttatgtttat attccaaatt atgaagataa
    13501 ctatcggatt gctacttcta ttaagctgaa cgaacctctt tctttgctgg tatcctggca
    13561 aaatacacct tcacaatttg gtatgggaat taaattaaag attaaaaggg gagagcttac
    13621 ctatgctatc agaacccatt cagaactaaa tctttctcat agccttgacc ttgggttcaa
    13681 ctggtgaaaa gctttgttgt catctctttc ctgttatctt taactttttt actttatgct
    13741 caatactacg atgaattaat tgaactggat actcctgctt tagtagattc tgcctggaac
    13801 aactattatc aggaactttt ggagcttgaa aagccgctta atattagcac tcgtctctat
    13861 cttcaaaata cgaataatta ctgtctgaac agtctacaat atgaaattcc tcagggaata
    13921 ttgctattaa ataatcggta taatatagat acgggaaata atgattgggg acttcatctt
    13981 aatcttaaaa cggaaagagt aaatttggtt ttgggttctt atagagttcg ttttggaaga
    14041 gggttagttt gcgggactgg ttctcgcaag ttaccagatt ctccttttaa gctttatatg
    14101 ccaacttctc cttctacata cactccttta ggtgcagctt ttatctatca aggaaactca
    14161 ttgcaaaccc tggtatttgc ttcggtccag aatagagtag ccaggttaaa agatgaaaga
    14221 ataacaagct taccaaccac taattcagat aatttatctc atactcagga aaatatttat
    14281 ggcacttctg tagcctgggt aaataactct tttcagattg ctggattact ttatagacag
    14341 caatatgata gagcttttaa cgattccctt ttagtatcta atcttaatgc ttacagtctt
    14401 tatgcagcct taaaattacc taataatcag ataagtacag aattatcata tgctaatcat
    14461 cagcttagca ctattctatc ctggaattat catttgaaat cattcagcca gactctctcc
    14521 tatgctcaaa acactatcta ccatcaattt ccctatgctc tttcagcttc tattttaaat
    14581 cataataccg accgaaaaga gataaactgt gatttgaagt ggaatgttct cactaataca
    14641 aatttacaga tacattatag tattaatata ggtcctgctt tttctggaga gcctttttcc
    14701 agattcattt cttctcttgc ttatgataaa agaggtactc gctggaaatt gaacttctat
    14761 cagtatgacc gagagttaat cacctggatt gattctactt atatttctac aatacctgaa
    14821 cattatagaa tccagactac cggtcattta ccaatcacaa cctatttgtt tcagcaattt
    14881 gaattttcct ataccctcca aaataaagag gattacacca aaaataccta ccatttccaa
    14941 tattttctgg gttataaaaa gtcaatctgg gaaatgagat tagggtatac cagctggcaa
    15001 agtccacata acttttatat agcagatgaa attagtcctg aatattacag tatgtgcagc
    15061 tctgaagaaa cagccatttc cattactact ggcttaaaat ttaaagaagc tgctttttct
    15121 ttatatggtg aaaagtctct cttatcttct aaaaaataca ggattaggat tcagctaaaa
    15181 gtatctatct tttagtttaa ttattccatc ctgctcttgc atttagggca ttctttataa
    15241 ctatcacctt ctctggtctt tttctccacc agataaggat ttccgcattc cggacaagag
    15301 ataggaaggg gtttataaga actgataaat ttacattcgg gataacgatt gcaggaatag
    15361 aagatatttc cttttttatt gcgtctttga attagttcac ccttaccaca ttctggacag
    15421 gtaactcctg ttgtcttatt tctggaatac ttacatttag gatagttgct gcaggcaatg
    15481 aattcgccat attttccctt gcgttctatc agaggacttc cacatttagg gcatttctct
    15541 tccagggttt gtggttctac aataacaatg ttccctttat catcacgaat gaattttttg
    15601 gtatttttac aggctggaaa acgactacag gaaagaaatt cgccattttt ggaatgcctg
    15661 attaccatct ttccttcttt acatacatca cacacaatat ctgtttcttc aatgaattcc
    15721 tgtttagatt tcttcacatc aaccttattt aatagattca tcagttcctc ataatagttt
    15781 tttaccagct catgccagat tactttattt tcttccacct catccagttt tgtttccata
    15841 tcggcagtga atcctacatt gaagatatca tcaaaattag agaccagaaa acgatttaca
    15901 tcaatgccta atttggtggg gaaaaagttc ttcttttcca taccaacata tcttcttttc
    15961 cggatagtac tgataatagt ggcataagtg gaaggtcttc caatgccttt tgcttccagt
    16021 tcctttatta aagaagcctc tgtatatctt ggaggtggag tggtaaaatg ctgagaagat
    16081 tcaattttcc catattcaag gttgtctcct ttctcataag cagaatctat agcttcccca
    16141 gatattatct tcatataagg ataaaccttc aaaaatgcct catcttctac cacatttcca
    16201 ttagcactga aaattgcctt ccccacagtg acttccacct tggttcttag caacttagca
    16261 ggtttcatct gagttgctac aaatctctgc cagattagag tatataattt taactggtct
    16321 ttggtgagat tttctgctac actttctggg gtgtaaaaag tattggtggg tctgatagct
    16381 tcatgggcat cctgagcagt agatttattt ttataaactc ggggtgcagg attgataaac
    16441 tctttaccaa atcgctcttt tatcaatttt ctacaatttt ctattgcctc atcagcaatg
    16501 cgtagactat ccgtacgcat ataggtaata agacctgttc tttccccttt aaggtcaact
    16561 ccttcataca attcttgagc tattttcatt gtcttttccg aggaaaaacc cagaatcttg
    16621 gatgcttctt gttgaagtgt actggtaata aaaggaggta gaggttccag cttatgagtg
    16681 cttctcttta aatcggaaat aactgctggg gaagtatcta tctcttctat caacttttgt
    16741 gccgtagcct catccggaat atctattttc ttattttccc atttttccag agctgcctta
    16801 aaaggaggaa gattatccct atggaatttt gcttctaatt tccagtattc cttgggtaca
    16861 aagttctgga tttcttcttc tctttcacag attaatctta acgctacact ttgcactctt
    16921 ccagcactta gtttctttgc aatgaccttc cataataaag gtgaaactgt ataacctaca
    16981 atacgatcaa gcactcttct tgcttgttgt gcatccactt tagccatatc tatggaagaa
    17041 ggatttttaa ttgcatcttg aatagctcga gaggtaattt cattgaatac aatacgaaaa
    17101 acttccttac cgcctagttc tttctccaga actttggtca aatgccaggc tatggcttct
    17161 ccttctctat catggtcact ggctaaatat atagtatcgg catctttagc tgcttctttt
    17221 agttcattga taaccttact tttcttctta tctatcctgt aaactggacg aaagttatgg
    17281 gctatatcaa ctcccagttc attttctggt aaatctctta tatgacccat cgaggcttta
    17341 acatcatatt tatgttctaa aaacttagaa atagtattag ccttagcagg tgattctaca
    17401 ataattagat ttttaggcat taggttcctt ttccaacttt tttatttatt cgtcgttttc
    17461 aaaacgtcca cagcatttct tatatttttt cccacttcca cagggacagg gatcatttct
    17521 accaacatgc tgagctaccc gaatgggttt ttgttttact accccagaat ctggttcaga
    17581 aaaattaggc ggttcactca tattctgttg accaggtctc aattcttgag atttcaggaa
    17641 tgcactaaca ttttcatgtt gcaatttgga gtctttcatc atattctgaa cttgttcttg
    17701 agtaagaata taggtggtaa acactttttt ggttacattt tcctgaatgc gagaaataag
    17761 attttgaaaa agtttaaaac tttctctttt atattctatc agggggtctt tttctgcata
    17821 ggcacgaaga tatactcctt ctttaaggag gtccatttca tgcagatggt cgcgccattc
    17881 ttcatcaact acttccagaa gacaacggcg ttcaatttct cgcattgatt cggaacccac
    17941 ttgttcttct ttcttttcat aggcttgcaa aatcagctct tttaaagtat tccttaaaag
    18001 aggttcactt agatggtctg attccaggtc ttctggctct atggctacat ttaaggcaga
    18061 tttgaaccat tgagtaagcc ttgccagatt ccattcatct ggggaactat caaaggaaat
    18121 attttccgag ataatcctat ctattgtctc ttcaatcatc tcttgaactt cacgctttag
    18181 tgaatatcct ttcaaaacac ttctacgata agaatagata acctctcttt gttgattcat
    18241 cacttcatca tattttaaga gattacgacg aatctcaaag ttattttctt ccactctctt
    18301 ttgagctctt tccaccgttt ttgtcatcca gggatgtctg atggcatcac caggtttcat
    18361 tcccattctg gtcatcattg gcgcaattct ttctgaacca aaaagacgaa gaaggtcatc
    18421 ttccagagat aaataaaacc ttgaagtgcc agggtcccct tgacgtcctg ctcttccacg
    18481 taattgacgg tctattcttc tactctcatg tctttcacta ccaattacat gaagtccatc
    18541 taaaggcaag ccataaggat attctggtgt tatggaaggg tctatacccc tatattcttc
    18601 aatactttta gtcaccaccc ccggtccaag tttgatatcg gtcccacgac cagccatatt
    18661 ggtagctatg gtaactgcac caggttgtcc tgctaaagct ataatctcag cttctctttg
    18721 atgttgacga gcatttaaaa cattatgagg aatattacga cgttttagca tacgactgat
    18781 agtttccgaa acttccacac ttactgtacc cactaagaca ggtttattca gattgtgcca
    18841 gtagataacc tcatcaatca aagcctgata tttctcattc ttagtcatat agattacatc
    18901 ttcatggtca attcgggtaa caggaacatt ggttggtatc gttattacag gaagattata
    18961 aatctctaca aattcagctt cttccgttgc tgcagtacca gtcatacctg ctaatctttc
    19021 atattttcta aagtaattct gcaaagttat cgtagcaaaa gtctgagtgc ctgcttctat
    19081 ttctacattt tctttggctt ccagagcttg atgaagacca tcagaatacc ttcttcctgg
    19141 cattaatctt ccggtaaatt catctacaat gaccacccga ttgtctacaa ccacatattc
    19201 tacatctttt tcaaaaaggg tataagcttt taaaagttga ttgatattgt ggagtttctc
    19261 acttttatcc ataaaccggt tggtttctcg ggcttttttc tttagttttt cctcttcagt
    19321 caaactctca tcttcatcta tgctatttaa aatttcatcc aggctttcta ctaaaaacag
    19381 atttttgtcg tgtttagata ggaaatctct gcccttttca cataggtcta cggtgttttg
    19441 atgctcatct accacatagt acagattgtc atccagttct ggcagcttct tgtcacgcaa
    19501 gtaaatacct tctacatcat tgaccagctt ttttagtttg gggtcctgca ttatcttggt
    19561 aaaagcttta tttcgtggcg cagctctttg aactaacaat agatttcttc ccaatctttc
    19621 tatatcagaa ttcggattat cttctctaag atcttcccta atctcagata aaactttctg
    19681 taccagtgtg ttttgtgctt gaactaattg atttatttga gggcgaagtt cctggtaaaa
    19741 atttttatcc tgagcaatag gtccactgat aatcaaaggt gttcttgcct catcaataag
    19801 aatgctatct acttcatcca caatggcata atagaaatct cgttgcacta attgttcagg
    19861 tataaccgcc atattatcac gaagatagtc aaaaccaaat tcactattca ttccataggt
    19921 gacatcactt tggtaagctt ttttccggga atcaaaatcc atatcggttg tgatgcaacc
    19981 tacggttaga ttatgaaaat taaatatggg agacatccat tcagcatcac gagcagcaag
    20041 ataatcatta accgtaacta aatgagctcc tcttcctact aaggcattaa gaaatagagg
    20101 caaagtggca actaaagttt taccttcacc cgttgccatt tcagctatct tgccatcatg
    20161 caaaaccatt gctcctatta attgcacatc aaaaggaacc atatcccata caacttcatg
    20221 ccctcgcacc ataaaagttt tccccaccag tctacgacag gtatctttta cgatagcata
    20281 aacttctggt aaataatcat ctaaggtgga tttagttaaa ttcttaagcg cttttttggt
    20341 ctcatcaatt tggatatcta tcctgttacg ttctgcttca tcttcttctt cacggtaacg
    20401 aatttccagt tcttccagtt tttctctcaa atcacttagc ttttcagcta tttcctgttt
    20461 tatttctgct actctttctc gcaactggtc atcatcatac tgcgctaaac cttcataaat
    20521 ctggtttatg agctgaactt gtggtaaata tttatttaaa tcacgggtgt ttttatcacc
    20581 aaacatttta cggagtattt tttctaacat ttttcaagtc cccattatat atctactaac
    20641 tgctaaagaa gcaaaataga tacataaatc atttcatttt ttctttaaaa actgattgcc
    20701 ctattatgtc aataccaaaa tctctttttc ttactgcaaa aaataacaga caagctgatt
    20761 tttatatctt actaatttat aatggataat aatttcttcc ttagctctct cttagctctt
    20821 tcttaccaat tttgtcaaga aggagataag agacagatag ttacggaatt tttatggtca
    20881 ttaaattaat ttttcttaat tataccacaa aaataatatc tgaacttgca taagttatgg
    20941 tgatacgaag caacaaataa agtggtatga taaattggat ttatatctaa acatctttct
    21001 ggtttaatcc acaacatttt tataggaaaa aaaacaaata attcaattga cataatctct
    21061 attttatttt aggagtattc tattgagatc aattttcctc tactttagga atttaatttg
    21121 catgataatt taattaactt agtagatata taataaataa ggagatagaa tgaaaaagca
    21181 tttattaatc atcctaatct tgagtttggt gacatttgtt tttgccgctc aaggttccat
    21241 taaggtgagc aacactccta ccgctgcggt ggtaactaat agtaatgatt atggattgag
    21301 tgttagctac agtatagaaa agcttgattt tcaggaaata cagacctcgg aagggttatt
    21361 taccgactta tgggtagctg attatgctgc caccaatacc ataggtcttc ccaagcttcc
    21421 ccttttgcgt cagattttca gtgttcctga aggatctgat gttcaggcaa gtatgaactc
    21481 agttttgcgt aagaatatag acctgaaagc tgaaggaata aataatccca taattccaag
    21541 acaggaacct gtatctaaaa gcgtggaccc ggcaactctt ccattcatcg ttaaccagga
    21601 gttttatcag agtaaaaact ggacagattt tccctctatt caggttactg atttaggtta
    21661 tatgagaggc taccatcttt atgctctgga ttttacacca gttcgttata atccaggaac
    21721 gggagaaatt gaagtcattt atagtgctga ggttaatgtc caatttcttc atccagactt
    21781 agtttcaacc aatgaattaa gagccaagac ttattctcca gtttttgatt ctatgcttaa
    21841 aactattttg atgaatccag attctaatcg ggcaagtctg aatcgttatc ctatgagtta
    21901 tttaattatt actcctccta actttgtgaa tgctttacag ccatttgtaa actggaaaac
    21961 ccaggaaggg tacaatgtga ttgtggcaac aactacacaa actggaacca ccactacttc
    22021 cattaagaat tatattcaaa atatctggga ttctgctact actgaaaatc ctgcaccgac
    22081 ctatttattg attgttggag atgtggcaca agttccagcg aactccgctt ctgcaagtcc
    22141 tggtggacat gttactgatt taacttatgt tcgcttgcaa ggaaatgatt ttatacctga
    22201 gatgtatttt ggaagattct ctgctaccac ggaagcggaa gtaactaatc aggttaataa
    22261 aactttaatg catgaaatgt atactatgcc cagtgatgcc tatttaagtg aagtcgtaat
    22321 gattgccggt gttgatgcaa gctggtcacc tacgcatgca aatggtcaga ttaattatgg
    22381 agtaaacaac tattttaatg ctgcacatgg tattaactct catacttatt tatatcctgc
    22441 ctctcagaat gcctcttctt ccataattaa tgatatatcc aatggagttg gctatgccaa
    22501 ttatactgcc catggaagtg aaacttcctg gtatagtcct tctttcacta ttgccaatat
    22561 taatagcctt caaaatgtaa atgaatatcc agtagtggta ggaaattgct gtcttaccaa
    22621 tgcctttgat accggtgtct gttttggaga agcctggctt agagcagaaa ataaaggcgc
    22681 cgtttcttat attggcggga ctaatagtac ctattgggat gaagattact attgggcagt
    22741 tggttacaaa ccacctatcg taggaacagg ttcacctttt gtcccaggtc atactggagc
    22801 ttatgatgca atgtttcatg agcataatga accttttgaa gattgggcaa atagtgttgg
    22861 tgctattata tttatgggaa atatggcagt agtccaagcg aatagcccac gaatcaatta
    22921 ttattgggaa atatattcca atatgggtga tccgagtttg attccttatt tgggcatccc
    22981 tgctcaaaac agttttgttc ctccagccac tctcttttta ggacttggtt ctttagatat
    23041 aactgctgat ccctacacat atgtagcaat ttcaatgaat aatgaactac atggagttgg
    23101 tttaactgat gcctccggta atttaaccct taatttcacc ccctttaccg aacctggtac
    23161 agctcagtta gtattaactc gttccttacg tagaccaatg attgccaata ttcaggttat
    23221 tccaaatgtg ggaccatatg taacggtaag ccaaattact gtaaacgatc ctaatgctaa
    23281 tgcaatagca gaagcaggag aaaccatcag tatgaattta acattccata atgttggcgt
    23341 tcaacctgct gctaatctta ctgctaccct ttctactact tgtccttatg taactatttt
    23401 agacggaacc gcaacactcc cggatattct tcctgatcag actatctctc tggaaggttt
    23461 atttagcgta cttattagta tttatatccc agatcaaaca tccgtcccct ttgatattat
    23521 tgtttctgat ggaactaaca cctggaccac tacgcgtagc attgtggtaa atgctccaaa
    23581 gctacaattt ggcgatccta ttctctttga tcctgatggt gatggcttct ttgaacctgg
    23641 cgaaaatata tctataggga taaccatcac caatattgga catatgaata cagatagtgg
    23701 aaccctaatt atcctggtaa ataatccttt tgtcactctt agcagtaatt actttgtgtt
    23761 gccaactatg gcagctggtg taaatatccc gattaacttt accgttactc ttgctcctga
    23821 tatcccggat ggaattattg tgcctataag cctttccatt tctgctggac aaataaatat
    23881 aaatcattgc atacttttac ctattggagt atatggagac ggttttgaaa cgggagattt
    23941 tagtgtttat ccctgggtaa ataacagtcc tataccctgg acggtggtaa atgatcctgc
    24001 taatgcacat tctggaaatt atgtagcaaa atctggggca ataggacata gtgcaagtac
    24061 tgaactttcc gttacattaa atgttagttc tgcaggtaat atcaagttct ggcgcaaggt
    24121 atcttctgaa ctcaattatg acttcttacg ctttagcatt gatggagaag aaaaaggttc
    24181 ctggagcgga accctaccct gggctcaatt ttcttatcca gttacaaccg gtcctcacac
    24241 tttcaaatgg acctattcca aagatagctc agtatcttct ggaagtgatt gtgcctggat
    24301 tgatgatatt accttcccga cctctgccag tggtgaaatt gctataattt atgtgcctca
    24361 gacagaattt agtttcttag aacttcatcc taacgatcag gtttcagcag atattgttat
    24421 caacaatgtg ggcacggtgg aacttactgg aactatatct tatccttccg ttatgaatct
    24481 tttatacaat ggatttcccg tggaaaacga tcataatttt agcattcccg tgggtgaagc
    24541 aagaatctac actttaacct acactgcacc cgaaacagct gtgaatcttg atttagaact
    24601 tataatcact tccaatgata ttaataattc ccccattatt attccgattc acatcactac
    24661 tgtgcctaat aatgatcctg caactattcc ggtggttact tctctggaag gaaattatcc
    24721 gaatcccttt aatcctgaaa ccgctatcaa tttctctctg aaagaatcag gtaaagttcg
    24781 tattaatatc tataatttga aaggacagtt agtcagacaa cttgtagata ctgaactacc
    24841 tgccggaaga catcagattg tctggaatgg aaaagataat caaggaagaa gtgtttccag
    24901 cggtatttat ctctatagga tggaagctaa aggttacact aatactaaga aaatgatgct
    24961 aatgaaataa aacttaaaga gggagactaa tatttaaatg tagtctccct cttttacctc
    25021 ttggctcata gctaaagagt ttttttataa agtcaatgga gaaaaaatga agaaaatatt
    25081 acttataata accctcctat taaccctgac ttttgcttta actgccaggg aaataactgt
    25141 tagcagttat gataatcaca tccaattatt ggatagtaat ccagaacata tggtaatgca
    25201 acttacgctc ggtcatttca attctgaacc agtagatata gatggaaaca cctggaattt
    25261 gattagcctt aaaaaggaag gtttaaccct tgaagctgga tatccgcagg ttcctgtttt
    25321 agcaagaagt gttattatac ccaacaccgc taaaatggaa ctaagcatcc ttaatagtga
    25381 atatgtagaa atgattttgc cagttgctcc ttccaagggt aatcttaccc gtgatataaa
    25441 tcccgaaatg gttccatatt attttgccga tttttattcc ggtaacgaat tctggcctga
    25501 gaatctttct tatctaacag aacctttcat cctccgtgat tataggggaa taaccgtccg
    25561 ttttcagcca tttgtttatt atccagccac tcaaacttta cgtatctaca ccaaactgac
    25621 agttgctctt gaggttaatg gcactgattt aactaactcc ttaccagtag ctaaaacttc
    25681 ctatgctcaa gagtttgcac cactttatga aaatatgttt ctgaacttta atcaagctaa
    25741 atatccttct ctggcagagc agggaagaat tctggctatt aagaactcta tgtttgataa
    25801 tgtaatgcaa ccttggattg attggaagcg tcaaaatggc tatagagtag atgtagttga
    25861 tgtcactgtt gctggaccta ctgccactca gataaagacc tacattcaaa accagtataa
    25921 cttaaatgat ggcttgatgt tcgtgcaaat ttttggtgat gctcctcaag ttcccactct
    25981 tacctctggt ggtggaggtt ctgatccatc ctattcactt ctggcaggat ctgacagtta
    26041 tcctgatatt tatgtaggaa gattttccgc ccaaacggtg gctgaaatgg aaactcagat
    26101 aacccgttca gttcactatg aaagagatat tcaagctgga agtcaatgga ttcaaagagc
    26161 tataggaata gcttccaacc agggaggtgg ttctcaaggt gatatgggtg aaagtgatca
    26221 gcaacatcag gaaaatatcc gaactgatct tcttaattat ggttatttaa gtgtggatca
    26281 gatctacgaa tctatgggag ctactgctgc tcaagtcagc acaaacctta accaaggaag
    26341 aggttttatc aattatactg gacacggtgc tgatacttat tgggttacca ctggttttag
    26401 caatacccat gtaaatgcac tggtaaatga ttataagctt ccttttattc agagcgttgc
    26461 ttgtgtaaat ggaaactttg taagtcaaac ctgttttgct gaagcctggt tacggtcagt
    26521 taatgaagca actaatgctc ccgcaggagc tataggattt tatggtagtt ccataaatca
    26581 aagctggaat cctcctatgc gagctcaaga tgaaactgta gatcttttag tagcagaaca
    26641 gaaacatact atgggaggtc tattctataa cggctcctcc aaaatgattg aggtttatgg
    26701 tcaggatgga atcagtatgt ttttaacctg gaatatcttt ggtgattgtt ctcttatggt
    26761 tcgttccaag gatccgcaac ctattacagc cacttatacc cctatccttt tcattggtat
    26821 gaataccttc ccagttaata caattcctgg tgcaaaagtt accctttata gtgctggaac
    26881 cgtatatgct acaggatatg ctgatgccaa tggagatatt aatttgactc tggaaaatcc
    26941 tcccctggaa cctatggatt tgactttgac tatctctgct tttaataaag taacctatct
    27001 ggacactatc caagtgcttc ctgccagtgg accttatttg gtagttgcag atatgatggt
    27061 gacggataat aacaataata tccctgaata tggagaatta ataactttga atttgatgtt
    27121 tagtaatgtg gggaatgaca ttgccattaa tcctactgtt tccattcaga ctgaagaccc
    27181 ttatctaact attattaatg gaaccgaaaa cattcctgat attgctgcca atagcatcgg
    27241 tggaacggtc aatggattaa ctatacaggt agcaagcaat gttcccgacc agcatactgc
    27301 ttctattcat attattgcta ccgttgctga tgaaagttat gaatatgacc gtaatctggt
    27361 gcttaatgct cccgcattta cctgggaagg attaattatt gaggattatc ttggtaataa
    27421 taatagtgtt ttagatcctg gtgaaagcgt tactatatgt cttcccgtga ctaatactgg
    27481 acatgctcag gcaaataaca tttctacagc tctggcaatc gtcggaggaa tgaatgtaat
    27541 tgaacctatg gacgattcct tttccgcttt agcacctgga gaacaatgcc aaataaccta
    27601 ttatattacc ctgagttcac aggttgctca aggaacaaac ttactattaa cagcaatgct
    27661 ttttgctgga gattatagtt ctgttcagaa ttatcctata accgtaggtt tattaatgga
    27721 gaactttgaa accaatttct ctggctttaa ttggcaattc tccaatggga actggactat
    27781 agataatacc agttataata gcacacattc tgctaaatct gccactatca atcataatca
    27841 gactacttca atgagtgtta ccctaaattg cccggtagat gggacaatca gcttctggaa
    27901 gaaggtctcc agtgaactga attatgacta tctccgtttt tctatcaata atgtagttcg
    27961 cagtcagtgg tctggcactt cagataattg gagtcaagtc tcctttgatg ttcaagcagg
    28021 acagaatact ttcacctggt cttattcaaa agatagtggt atctcttcag gtagtgattg
    28081 tgcctggatt gatgatatta ttttcccctc cagtggaaca attattggaa caccggttat
    28141 agctattgat accatggtag tagattttgg cgttgttccc ataggtcaaa ttgctcaacg
    28201 cactttcact gttaataata acggggatgc cgtgttaatc ggtactgttc aggtagctcc
    28261 accttttgct gtttatcagg gaaatggata tcctggattt gctatcaata ttgtagttcc
    28321 tgctcaatcc tatctgggct ttaatctgaa ctttactcct gtggatactt tgacttataa
    28381 ttctacgatg cagattaata ctgatgaccc agtgaatcct gttattatgg tgcaactggt
    28441 tggaaaggga acggttgtct ctaacgatga tcccttattg cctgcagtta cagagctcaa
    28501 aggtaatttc cccaatccat ttaacccttc aaccaccatt gcttacagtg taaagaatac
    28561 tgctcccgtt agcattgaaa tatttaatgt taaaggacaa ctggttagaa ccttagtaaa
    28621 tgaagtcaag accgctggaa actataaaat tgtctttgat ggtctggata ataaccatcg
    28681 tccccttgcc agtggaatat atttctgccg tatgcaagct ggagattatc gtcagaccca
    28741 caaaatgtta atgttaaaat aaggcaaatg tagcttatcc tctccttatt tagcatagaa
    28801 aaaccgcctt cagtaaaaac cactggtaat atctggaggc ggttaatttt atctggttta
    28861 tcccaattca ggataaagag gaaatcgtgc ggtaagtgct cgcacctctg atttcatatt
    28921 agttagatat tcttcatctt caatatgttc tctcactctt tgaatgaaat cggcaatctg
    28981 tttcatctct tctattccca taccacgcgt agttacagct ggtgtgccca aacgaatacc
    29041 actggcaaca aatggactgc gagtctcaaa cggaacggta ttcttattag ctgttattcc
    29101 tgctttatcc agagcctctt ccattttctt accgctaggt ccaccttctt cttctgaacc
    29161 aagattaatt agcattagat gagtatccgt gccacctgaa accaagctaa aaccattagc
    29221 gattaaagca gtagccagcg cttgagcatt atcaataata tgttgctgat aaactttgaa
    29281 ttctggctgt aatgcttcat taaaagcagc tgctttccca gcaattacat gcatcagtgg
    29341 tccaccttga acgccaggaa atacttttga atcaatctct ttagcaaact cttctttaca
    29401 gagaataagt cctgctcggg gtcctcttaa agtcttatga gtggtggaag ttactacatc
    29461 agcataagga acaggattca tatgcaaccc tgctgccact aaaccagcaa tatgagccat
    29521 atctaccatt agcttagcac ctactaaatc agcaatctca cgaaacttag caaaatccat
    29581 ctttcgtgga taagctgaag ctcccgcaag aatcatctta ggcttatgtt cttccgccag
    29641 acgagctaac tcatcataat caagttcctc tgtttctttg ctaacttgat atggaacaat
    29701 gttatagaat tgtccactaa aggaaagagg atgaccatga gttagatgac caccatgcga
    29761 taattccaga gccataacgg tatctccagg tttaaccaga gtaaaataag ctgccatatt
    29821 tgcctgagaa ccgctatgag gttgaacatt ggcatgctct gcaccaaaaa gttgtttagc
    29881 cctttctata gccaatcttt ctacttcatc aatatattca caacctccat aataacgacc
    29941 ataaaggtta taattgattt tcccggtctt tttgctccag cgatagggat agccttcagc
    30001 atatttattg gttagcacac taccctgtgc ttccagaact gccattgaag taaaattctc
    30061 tgaggctatt agttccagat tttccctctc tcggtttaat tcacctgtca ttgcagcata
    30121 aagttccggg tcctgctctt ggatatgttt cactgccttt ctccttctat ttctttaatt
    30181 ttatccaccc taatctgatg cctacctccc tcaaatgaag tagttaacca ggtatttaca
    30241 attaccagag cataatgtac cgctataaat cttccagcta aacagaggac attagcatca
    30301 ttatgcattc tggaaagcct tgccagatca gcattcatac atacagcagc acggattcct
    30361 ttaatcttat tagcaactat gctcattcct attcctgttc cacagattaa gattcctctc
    30421 tctgcttttc cttcagctac tgctcgggct gctggtaatg ctgtatcagg atagtccata
    30481 gcatctaccg aataagtgcc ataatcttga aactcgtgtt cgggaaaatg acttttaatt
    30541 gctcccttga gttcaaatcc agcatggtca ctggcgattg ctataatcat atttcacctc
    30601 ccattaaaca tgatagtgct atacaataaa acttagaagc ggcgctttcg cttctactca
    30661 taacaattac cggttttata gtaccttgaa tcaaacctgc cagttctccc ttagcaaaca
    30721 tcatcaatcc tttataaaaa ctattggcac tctccaaact gggaaagatg agcacatcag
    30781 catctccagc aagaggtgaa ctgactcctt tgattatact tgcttgagga tcgcaggcaa
    30841 gaaagatatc cagaggtcca tccaggacac aattttttat ctgaccgcgt tctgccattt
    30901 tacagagttc agcatattcc agtgtatgcg gaatattctc ggaaaccttt tccgtagctg
    30961 aaatcaaagc cacttttggc ttttctattc caaaacgccg tgccattgct acactataat
    31021 ttatcattgc tatcttctga ttaatatcgg gataaggtat taccgcagta tcacttataa
    31081 agagaagttt atgatagcca ggcaattgca aagcacaaac ataactcatt atggcttttt
    31141 caggtaataa tcctttttct ttatccagaa ccgctttaag aaatttatcc gtacctacta
    31201 accctttcat taaaacatca gcttcatcat cacgaaccat ccttacagat tcagtcactg
    31261 cttcttcctc atctctggta tcaatcacgc gaaagattgc tggatttatt tgctcagctt
    31321 tacagagttc ttctattttg ggtttagaac caactaatat accctctaca aatccttcat
    31381 taaccgcttt ccctatagag gctatagtat ttggatcttc tgctcgggca actgcaattc
    31441 gggtgcgttt tcctgtggaa cggacataat tggctaactc ttccagagaa cggatgggct
    31501 ctttcactgt ttttctcctg ataaaaggga tgccagagca atagagtaaa gcttggattt
    31561 ttcactgtcg ccacgagaag tgagaacaca gggaaccctt gctcccataa ctactgcaca
    31621 aagttcacct ttcatcaatt tgactacgga tttataaaaa gtgttccctg cttcaatatt
    31681 aggaaacaga atacaatctg catcaccaca aacttcactt tgaaccttct taatttgagc
    31741 actttcttta tccacaatta aatcaaatcc taaaggtcct tccacgattg cacctttaat
    31801 ctgaccacgt tgtgccattt tggctatcat tgctccttca gaacaggaag ggattttagg
    31861 taaaacttgt tcggaagcag cctggatacc tactttagga ttctcaatac ccagataatg
    31921 agctatcttg attagataat tggtaatagc aattttctga ttgaggtcag gtagtggaat
    31981 tatggctaca tctccaaaga ttagaagctt gtgataggta ggaatttctg ctacggttac
    32041 atgggaaaga atggcacctt tatccattaa ccctttctct ttatgcagga tagccttcat
    32101 ataacgatcc gtgctaagta atccttttag caaaaaatca ccctctccag cattaatcaa
    32161 atctactgct tttgctgcag cttcaatatc tgactcagag tgaacaatct taaaccggga
    32221 tgggtctatt tgttcagtct cgcagacctg atatatctgt ttttcatctc ctactaatat
    32281 gccttctatc aaatccatat ctacagcttg atagacagca catatagtgt gtggatcaac
    32341 tccccaggca gctactaaac gtttacgtgg tagtggcttg atgaggtcca gaatctgttc
    32401 caatttggta acttgcattt gtactcctta tttatttata tatccaaaat gattgttcac
    32461 ccatgcatcc taaatgcatt gctaatccaa tatctctatt gcggttccct taaaagtatt
    32521 gagaaagaat cttctttgtc tgaaacttga tactttaaaa ccattataaa ctcataactt
    32581 attaatatat caatcagcat cccattcaaa ttcttttatc tcaagataaa ttttatataa
    32641 ttatgattta attgtcctgt taactattat ttgtcaagtg caatctccag atacctgatc
    32701 tttaaaatgt ttttctgccc tttggtgaat agattttaac tttaactaaa tttcagaaat
    32761 tggttatacg catttcttta tattatttta acagttattt gtttagaaat ccttatactc
    32821 agtatataaa atgaatctct aacggcaaca accaattttg gctcataatt tttatttctt
    32881 agcataaaaa cctgagcacc ggcatataga ccaagcttag tagttttaag attaggctat
    32941 gtattgtcgc tgcttatcac actctaaaaa tggattttat agtcagagaa taagttatta
    33001 tatcccag
//
LOCUS       DAORZF010000012        32758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_1121_length_32758_cov_359.372749, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000012 DAORZF010000000
VERSION     DAORZF010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 32758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32758
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1121_length_32758_cov_359.372749"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4395
                     /locus_tag="PK015_02645"
     CDS             <1..4395
                     /locus_tag="PK015_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33406.1"
                     /translation="DFMDLILEYRDLRYQEQSYFAEAENKAEITLKEKYNSLVSAKAT
                     LLRLYENYIKKHPHFEALLQPDGNYLVSNAMLYYNMAELQYAIDLDHPDKALTYYKKV
                     LEIDPNYYKKDYVLYNIGYLSSESIKSTLDNAIENYRKTHPYKDRPSNLKYTEQAFKE
                     AIDAYTELVDSGLYTNSPLYDEAIYRLGTIYFLIGSDADEPINYYNEAIRRFDTLVSK
                     PESDYYYHSLHQRGWVKLNLGDEASLQSAIFDFTNLIEAVDIGEIEDPYLASDYKNNS
                     IDNIAYALVALDGIDFINPAKGIQFFEEILINHPDTLVKSEIIDKAVSLKMEINAPLQ
                     AIDYLELRLKVFPLDLQNPSIIDSIITIYHTPGLVLREGNDLTTICNAEYDFIKANYN
                     NHSSWYEHNIKNAKLDNLEIRKQLDIIKRAYEQIRIRYYNQIIDSIADSDLQIYNQHL
                     QEYIDYYELFKDSTDFITWQKEVQRTNTILYTYLAEKRDTPVDYYIAIQTLYSYNETN
                     PDNPNYLNNEGLAYRYARKIYDYLEEEFKHTNLKTDPLLPTNKDELYNFYEAAALRFY
                     NHLINTEDEGLRKSAIPILMDLAAVALENGKDYIAQQYYQQLLNNADQLDSPTLRTIY
                     INLAKIAENNLNYTEAKLYYEKAKDYALNAHDSQELEHLIRLQLQNSYEEAERNGDYL
                     KMALDLEELANRFMDDPEQSKAYKYQASEAYKKAGNYQKAIDIKLQLAKSKNSIEEKY
                     FLYYESWNIADSLMKNHSYARQLQEEFTAMYPSSNQAFNLKIAMIEELKSNPAQREDA
                     AQMYLQLYQDVRSGKIDSGNIPLEDIYLWAVNIYLEDQNQDKALELLSNFITLYPNYN
                     KATDILTFLADTYLARGEEDKFESYARKLYQKDKSQSERYLTIAQLKLGRLAHEFDDA
                     YKNKDWNLAFQKRDEFIKLEAQYLKDGLPINNEAAYEAFAYAEAEYKNEQARKAYLDN
                     FDRQLKTIANGELLTSSANKLIPVNTQTTWRGSLFEKSPRYIPSLKKRAETECAKIIH
                     LLEQPEADFLDIDRRLKALCLIARINEHTAEVIETQLDKYFQISNEMAPFRDRKKYSE
                     EEYNNLVYGQLMPYAQQFIEPLRTAAISIYMEIYNNYNVAGCVNDYTNLAELKLLEQN
                     CLPNYYEVAFPLDQNWNLNLQLPDGKFKKVNPYTESIMSPKGIQLFSIQIPAHNALIM
                     EREFTFEYPPEFAFLHIVYPDNPEIWLNENPIDLIYIPVDTLITGDLSTIHFAAKINK
                     QAWKQGYNEIKYIFSNNFDKPLSFYFNSNLFFDADMIDEFSLLRTKRIITNTNWNALI
                     KDNESGKETKTKTIKAFTFDLPLDRSELLTDTSVKPIWVEENSDHPYNNITFETEFIL
                     DEEVHYAYIDLVAPEIATIYINGTKIVQKLPLNFDSFPFMVYPSRIEIPPEVLRKGKN
                     ILRIEILNNSPFRGMMAEMNFNLSTTGE"
     gene            4399..4695
                     /locus_tag="PK015_02650"
     CDS             4399..4695
                     /locus_tag="PK015_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33407.1"
                     /translation="MNRFWFICCMLIFMMPLCFAQVKSIASPASNDGEIIKELEIQIK
                     TMLPDINIVRLKTDTYISAGQYNYTKYKDLIKAENIKNLIKLQKDITKQPEESK"
     gene            4692..5471
                     /locus_tag="PK015_02655"
     CDS             4692..5471
                     /locus_tag="PK015_02655"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOL33408.1"
                     /translation="MKFKHILLASLILIIGIGPLLAQTAQNPYNIPPGTEKITLASIF
                     RDSNAHGIFGYLILLTFTIGIVYAIIRYIQLYQKEKVNAPNLYKSLKGYIKNNQIEEA
                     IKITEKLKDTTLGYIFWNGITAYKEIPKDASKEEVSTNVQNAFDEAALQKVYKLDAGL
                     FWFDILAQVCTYLGLLGTIWGLLYAFAAISNPTVLDTQKNLMLSAGIKTAIGTTALGL
                     IAAIPLTLIKGALQSRAQKIINDIDEFSVKFINFITSLAKG"
     gene            5476..6021
                     /locus_tag="PK015_02660"
     CDS             5476..6021
                     /locus_tag="PK015_02660"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOL33409.1"
                     /translation="MAHPPSARRDQHHFSPPKVSDLVPLMNLFLIIIPFLLLMMVVTQ
                     IAMIALNFTPGTGTEKQAGGPLNGTSVTQKNIPEITIIIMASQDSLGTTFPGFEIREP
                     EKTKDKVGLVNGYYDYVKLDEKLKAIRQRYPDLQDISVAPYDDVLYDTLIKTIDLCRE
                     NNFSNIHYKAPQVRYFAREVK"
     gene            6018..6572
                     /locus_tag="PK015_02665"
     CDS             6018..6572
                     /locus_tag="PK015_02665"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOL33410.1"
                     /translation="MNQSKYSRDLKHLVPQRHKDEWSLSIISLIDILTILLVFLLANV
                     TVEGQKFTQPNRMTFPVSMKKRDLLEKKGTTVVQIYPDRILIGEKGIYFGTLNEFAND
                     PVKRTEILKYLQTTALQILEDKDESGNPRTPPTLLIQADRAIPCWYITEFVSLGTSAY
                     YEYIYFATLLDANWFQHSVLSTRR"
     gene            6584..8071
                     /locus_tag="PK015_02670"
     CDS             6584..8071
                     /locus_tag="PK015_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33411.1"
                     /translation="MVNKILTLKLFYKGKLLDIIEQEKDFTNKWYIGSSKYLSWQILD
                     ESNKFPPKYKILIKKGGHYYLNLPQGSTLTCRKNDSPVDANFLKQNGILVDSLLKLRE
                     DMTGIIQVHPDYSIQYEFIEPVKIILTPQEKEVIKQTARPAPLAPMDKTTRVVVILAL
                     MAGIAFSIFYDLKLKPEMFREQTLSELLAEIEKIREIKPEISQSQTPSTPLTTLPEEP
                     ATSEKTTKNAQVGKGKSTGKGGSSTPSGTQSIFGDLGTSSGTYAGGSTQSVVGAAVLS
                     GFVTARPGVKGGSGIGNASGTFQPSAAAGLGSGGSFDPNAINRFNAGDVPSVVSGTAP
                     EGGSTVKPSGSAQYYTGPAEKLKPLAGGSLTSVPDVRAETEKIVSQSYKASEPAKIQE
                     PAVTIVKPSTIVAGGDIIFNQIRSRKGQIEQAYKRNAAIKKQSGSITVILDIASDGTV
                     KATIIPNSVTFTESFLNEIKSIIESWTFNVSQPTTYKFKMNLSQG"
     gene            8518..9150
                     /gene="infC"
                     /locus_tag="PK015_02675"
     CDS             8518..9150
                     /gene="infC"
                     /locus_tag="PK015_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOL33412.1"
                     /translation="MPKERINEQITANKVRLIGADGKQIGIVSLKEALHRAEAEDLDL
                     VEISPNADPPVCRIMDFSKYYFEKEKKAKEARKRQHEVEIKEIKLGPNTEEHDYNFKK
                     NNAIKFLKQHNKVKFTIRFRGRQMAHQELGLNVLERIKADLGHIAEPETEPVAERNML
                     YIIMSPKKDIDRILAKMEKEKAALEGKTVPTTQDKANSSTPAEPKKENLK"
     gene            9197..9391
                     /gene="rpmI"
                     /locus_tag="PK015_02680"
     CDS             9197..9391
                     /gene="rpmI"
                     /locus_tag="PK015_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007840008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOL33413.1"
                     /translation="MPKIKTSSSAAKRFKVTGTGKILRHHAKSSHIKTKKSPKLKRHL
                     RSVTLVSKADEKRVKRMLGI"
     gene            9404..9757
                     /gene="rplT"
                     /locus_tag="PK015_02685"
     CDS             9404..9757
                     /gene="rplT"
                     /locus_tag="PK015_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOL33414.1"
                     /translation="MPRATNNVAAHRRRKKYLKAARGNYGRRSNTYRIARQTVERGLI
                     FSFAHRKQKKREFRRLWITRINAACRNNDMTYSKFINGLHKANIAINRKTLAYLAWHD
                     SEAFARLVEIAKEAQ"
     gene            9786..10823
                     /gene="pheS"
                     /locus_tag="PK015_02690"
     CDS             9786..10823
                     /gene="pheS"
                     /locus_tag="PK015_02690"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOL33415.1"
                     /translation="MIDDQLKNIAEQAKIDLKECLNQNDILNIRAKYLGKKSLVNSLY
                     SQLRNLPEEERPSFGKKVNELRNELERMIDEKEKELKLQIVNAYDSVQANDLTLPGVY
                     FPPGSLHPLTIIRRKMEDIFISLGFQIAEGPDIEDEYHNFDALNTPEDHPSRNLADTF
                     YLDNGYLLRTQTSTVQVRTMESYPPPIYIISPGRCYRNDKPDPSHSPVFHQVEGLVVD
                     KGISMADLKDTLQFFAKAMFGPEVKSRIRPHFFPFTEPSAEMDISCVSCQGKGCRICK
                     NSGWLELAGAGMVDPSVLEMMGIDDQIYNGYAFGIGIERIAMLKYNIPDMRILFENDV
                     RMLRQFSRELI"
     gene            10820..13342
                     /gene="pheT"
                     /locus_tag="PK015_02695"
     CDS             10820..13342
                     /gene="pheT"
                     /locus_tag="PK015_02695"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOL33416.1"
                     /translation="MIVSTRWLSKYIDLPETLEELVQTLTFTGIEVEAIQEISALPET
                     VVTARILNSEKIPGTEHLQVCIIDYGKEPLQVVCGAPNCRSGLITAFAQVGSKLPEME
                     IKKTIIHSVESAGMLCSEKELGISDNHSGIIELPPETPVGIPINEIYELPDNILELEI
                     TPNRPDLLGYIGIARDLAASLNKRLKLPSINNYPGEEDSSLHLTLILQEPQKCPRYTA
                     RIIDGISVKESPLWLKNALIKSGLRPINNIVDITNYVMLETGQPLHAFDYHKLLPINQ
                     QESHPAIIVRRAKAAEEFIALDGNKYLLDEEDLVIADGQRPSALAGIIGGKDTAISNH
                     TTTIVLESANFSPSTIRKSSLKHKISTDSSYRFERHLSPAYLEMVSNRALELIINVAG
                     GRVCNELYDAYPVPQKPLFLAVRPSRFEELIGYKLDASLINNYLQSLGFQYILCGLWQ
                     KEPINEYKLLPQAMELDLETSDNKIFYENPQLALYFLVPSYRVDITREADIIEELARL
                     DGFDKIPQKKLIHPIMDWHAHHIKRKIEDYFRQSGFYEMINPSFIDPVKLEYLGEDKA
                     ELEKRLIRIVNPQSSNQSAMRTTMLPQLLDNLLYNLNHSERNLKLMEMGKLYWKDGNK
                     NCETLHLTALMTGLNNLDHWKVKNEPIDLYNVKGVVEGLLDQLSITIDETKNVHHPWL
                     VESDNLAYFCNNHLCASFGRLKAKTAERFNIDIITLKQDIWVLDFEVNTLIELTRTNK
                     IEFSLLPKFPAVIRDISFLINFSVPYADIVKSIKLVEPYLIQDVKIFDEYRGKGVPDG
                     WHSISLHIILQDYEKTLTEERVGQVIDSVIKMLENTWQIKMR"
     gene            13349..13672
                     /locus_tag="PK015_02700"
     CDS             13349..13672
                     /locus_tag="PK015_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33417.1"
                     /translation="MESNVINLQEKIQKLIDQYTADRKRLEQLEEENNNLREENRQLM
                     EQIENTKKFHSGSELKIKELENQVKLLQAQYKELQDSLAGLEEIASTTINQIDQLIPD
                     LDNKK"
     gene            13675..13944
                     /locus_tag="PK015_02705"
     CDS             13675..13944
                     /locus_tag="PK015_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOL33418.1"
                     /translation="MQSVEVEIFGRKFRFRTDEPEQTQEIASTINEELNTLRELYENL
                     DFTRLLLLLALRKQDEIIALRKQNLELEKNLERVNQMVEKIVGEI"
     gene            13947..14119
                     /gene="ssrS"
                     /locus_tag="PK015_02710"
     ncRNA           13947..14119
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PK015_02710"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            14169..15737
                     /gene="rny"
                     /locus_tag="PK015_02715"
     CDS             14169..15737
                     /gene="rny"
                     /locus_tag="PK015_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOL33419.1"
                     /translation="MQWDKVIWLAIGVAGGLLIALVIYLAKRSSSFRIISQAKKIAEE
                     IKESAHKEIENAKKAAVLEAKDEWFKQKKVMEDELKERQKELRDQERKYNERLSNLDK
                     RLDALDKKENNLLGQEKKLKYKEEEINRKYQECEQFINAQKAKLEEIAGMSREQALQM
                     MREELITQARQVAANDAKATIEQIKLDATKKASEILSTAIQRLAVDYVSETTVSVVPL
                     PSDEMKGRIIGREGRNIRTFEKASGVDLIIDDTPEAVVLSCFDPVRREIARLSLEKLI
                     ADGRIHPGRIEEVISKTSKEMEETLIKIGEKAVLDNNIHNISANLIKILGRLNYRTSY
                     GQNVLKHSMETAWICGMLAAELGLDQEIARRAGLLHDIGKAIDQEFEGTHAIIGANIA
                     RKNGESKIIVNAIEAHHEEVEPISVYAALVQAADAISGARPGARREMLETYMQRLTKL
                     EEIANSVQGVNKSYAIQAGRELRIIVEPTLVEDAQAALLATEIAAQIEKEVQYPGQIK
                     VTVIRETRQIAMAK"
     gene            15734..16546
                     /locus_tag="PK015_02720"
     CDS             15734..16546
                     /locus_tag="PK015_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HOL33420.1"
                     /translation="MMKVLFFGDVFGKAGRRIVCDYLSELKEEFNPDFVILNGENLAD
                     GRGLTEKTSKALFNAGVDAITGGNHLWDRAESWDYIKNNPAIIKPLNYPASAPGNPSY
                     TIVKNDKKLTIINLCGQLFMPPTNSPFIAFETWLNSSYIYNSSCILIDFHSESTSEKR
                     AFGWFVDGKVSAVIGTHTHIQTADEEILPQGTAYITDAGMTGAHNSVIGVKKEIIIEK
                     FKHCLPLRYESSDLGPQINAVYLELDNLTHKAQKILRIRRPIEYKNSIFNEE"
     gene            16551..17459
                     /locus_tag="PK015_02725"
     CDS             16551..17459
                     /locus_tag="PK015_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HOL33421.1"
                     /translation="MEKILSGKPLAKLIQSKIKFLIAQENLQPKMALIQIGEDPASSY
                     YVQNIINIGNKLGCEVLYNQYPADFTQEDLLTILDDANTDPSINGIMIQKPLPPSFDE
                     LKIAESINPKKDIDCFHPVNLGRIILEEDGLFPCTPLAVICTLRYYNIETQGSHIVII
                     GRSNIVGKPLSNMLLWKKPYANATVTVCHSRSKNIKELTRSADILIAALGRPSFVTED
                     MIKAESVLIDVGINQVTDKDGKQSYVGDIDYNTCFDKAAAITPVPGGIGSVTSSLLFL
                     NLLKACFTAHNINKSIDDFLNYIFVE"
     gene            17496..19892
                     /locus_tag="PK015_02730"
     CDS             17496..19892
                     /locus_tag="PK015_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33422.1"
                     /translation="MLTKSKRLLIMTIWVSLILLVLTGCGKSGHKFANQEPTIRITSY
                     EGYDDSELLAHYADSLFIFQQKIFWSASDPDGVITGYAYRVKDQNDNPISTPGNEYID
                     LNGDVTPENVIERFGTGWVLHYKPNANQDLPLNDPNSRRTIWTSAKYAVINFPAADEF
                     GNPITKESTFEVIAIDNRGGITPVDPSYHKRSIAWRNFKSTSARPTCFLSTTKGNPNG
                     EEVGSGIRLSFTMKDELNPSVPAIPYKFEFKIIKLQPDSTTIISESDWYDTSISPNDT
                     NINKFLLTRYTTPSLSYDIENGVTQSITKVVARTYNMAGVTSETDGYSSISFKVKAGF
                     RPKTLIYNKRCYALGQNHYVDYYDESSAEVMPFTITSGTQRYATHFFVDLDSTLTAIN
                     SNNIKVWLRWGWHGEYGKILASGDYVVNDDPYERKIDYLLDRFTDKNYYSEITHFDLR
                     LDGQPYNYPPLANDPNAHIVDEDGKAWIRLPLYSPVRQTLVLTSLSSGLHTFEVRCVD
                     LQGEVDPIPAVFTFLLVDNLPIANRNGILIIDDDPNHPSVAPDAIIRAKYEEMLADYN
                     GPKDFIKRKTDIEPGDTHQDLSLRHLAPSDLQKYKLVIYHNDNFQSSGNLKLENDGLT
                     LYLRSGGNLIISSTNTLAQNLDAFVLAKQKTIISFLGVYYESPPASILSNALTIRPFF
                     QQALGQNGYPNVNLQYSAANEPSFVNLINNYQGLATITYFPKISVNATAIYKMGIKPV
                     GYTPNGPTPEQFNTYNNQIIGIRCVTGNSHCYTFGFPLSYMYTPDVKTMMNKILSEVM
                     "
     gene            20524..22536
                     /locus_tag="PK015_02735"
     CDS             20524..22536
                     /locus_tag="PK015_02735"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HOL33423.1"
                     /translation="MFQGNRFEVKLDAEFPPDPVFDPQYRRAPHRGLNLTPSEITLAV
                     KNALRYVPSEWHSRLAPEFLEELLTRGRIYAYRFRPQGRIWGRPIDEYKGITPARGIQ
                     VMIDNNLDFEVALYPYELVTYGESGQVCQNWMQYRLIKKYLEIMTEEQTLVVQSGHPL
                     GLFPSRKEAPRVISTNGIVIGKWDNPEDFQRLTALGVANYGQMTAGGWMYIGPQGIVH
                     GTYITLLNAGRKYLGIPSDKDLRGVLFISSGLGGMSGAQPKAVEIAKGIGVIAEVDYS
                     RIETRLKQGWVKKVSSNLEEIFQWVDEHRKTKEPISIAYYGNIVDLLEYVDSHNIKAE
                     LLSDQTSCHAVYEGGYTPVGLTFEEGRALLASNPEEFRRCVDTSLRRHFQVIKRLCDK
                     GAKFWDYGNSFMASVFDAGEPSIARNGIDTSEGFIFPSYVEDIMGPLCFDFGYGPYRW
                     VCLSGKDSDLHLTDQVAAEIIDPNRNTMDKDNHYWITHAEENNLVVGTKARILYADEE
                     ERIRIALRFNQLIREGKLGPVMIGRDHHDTGGTDSPFRETANIKDGSNRMADMAAHCF
                     AGNAARGLTMVVLSNGGGVGVGKAFNSGNGIVLDGSEYIDNVIKSGLSWDVMGGVGRR
                     NWARNENAMKTVRTWNEVHSEEGHITMPENIDDEMVNEIVQKRLQK"
     gene            22557..23921
                     /locus_tag="PK015_02740"
     CDS             22557..23921
                     /locus_tag="PK015_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HOL33424.1"
                     /translation="MLNQFIRMMKAESIPESVIRTFCIYYNQLVKGERGIIPSSEINP
                     PKEDNLLEYSDLNDYRKQSMLKQIVVIKLNGGLGTSMGLSAAKSLLPVKGNMNFLDII
                     VRQILLLRVTTGYDVTLMFMNSFHTDEDTKKYLQKYPDLEKQSLPVTFQQNKFPRIRQ
                     DNLKPYDNPDPKKRWNPPGHGDLYTTLYCTGLLDKMIDAGFRYIFVSNSDNLGATIDP
                     AIPSYMAANDLHFVMEVCHRTEMDKKGGHLCEDKNGQLMLREIAQCPEDELSEFQNIG
                     LYKYFNTNNLWIDLKALQWYLLTEDGKILLPLIVNPKVVDGTPIYQLETAMGAAISLF
                     KNSRAVVVPRTRFSPVKKNNDLLAIWSDLYELNDQYHIVLKQGIEQVPTIELDERYYG
                     NINQMMEHFKDGVPSLIRCKELKITGDISFGEDVICEGKVSLYADHPVYIKSKILTGE
                     IVFN"
     gene            23925..24467
                     /locus_tag="PK015_02745"
     CDS             23925..24467
                     /locus_tag="PK015_02745"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOL33425.1"
                     /translation="MINSIPHLADLKLSLIVAMDRNGLIGYQNHIPWRIPEDLAYFRE
                     KTLNHTVIMGKNTWMSLGSTLDQRVNIILTHDHNFYVPGAIVCHSVSECLEHCRPDDE
                     VFVIGGSQIFKLFLPLLSKMYVTRIDAEFEGDTWFPEINWEEWELIFFEQKKSKTGYM
                     LTFSEYVRKNRNWKPELPEE"
     gene            24553..25833
                     /locus_tag="PK015_02750"
     CDS             24553..25833
                     /locus_tag="PK015_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOL33426.1"
                     /translation="MKIYRISAILILIAILFGGCGQTVQSGKVQVRFWHGLGGPLGDV
                     LNDMIMDFNHTHKDIEIIANPISSYNALSQKLSAALQVGKQPDLAQVFESWAAKYQED
                     NVLVSIDSLIANDPDMTEEDLNDFYPVFMNSITYKGKKWSFPFNKSVRAYFYNKDAFY
                     RAGLDPYYFPATWQEFREYCKILTSPKDDIYGANFTVNEWQFVNLIHQAGGTIMDENN
                     RPVLNSQYGLEALNYILGLVNVDKTAKSTKELDGQNAFLAGKVAMYEGSSVSITFMRQ
                     QPINFAVGYAPLPTYRTKKSAISGTNIVIFKSGNPKKEKAAWEFIKWFTSTERTAEWS
                     ARTCYMPLRKSAMQTQIIQDFLAENPQFKDVYAQLEDAVFEPQHPAWFLGRDELKKSL
                     EKATAGILTPQQALDEVAAKLEKLVKEEEKKGKL"
     gene            25933..26020
                     /locus_tag="PK015_02755"
     tRNA            25933..26020
                     /locus_tag="PK015_02755"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25967..25969,aa:Leu,seq:caa)
     gene            26195..27232
                     /locus_tag="PK015_02760"
     CDS             26195..27232
                     /locus_tag="PK015_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOL33427.1"
                     /translation="MMSKYTILILATMMILLSLPFTVDAISQGALLFLTFEPGARANA
                     LGRAYSAVADDAYAAWWNPGATAFNRKTQLAGTHIPWLQGAGGGFDDMFYEYLGFNHY
                     FQDIGNVNVQFILLDAGTQEQTDEQGNQLGTFHSFDFAGGAGYSYEVIPEHLGVGGNF
                     KLIYSYLGPGTGNTEDEGKTFTFAFDIGAKYRDLGVKGLDLALAIQNIGPNVTYVDES
                     QSDPLPMTFRLGASYQVLDTPMNKLIVSAEASKILANDDPLFKRFITGWEHFDETMYG
                     FGAEYTYLNLISLRGGYFADQAGSIVGPSFGAGIQYTFSKKYKLTADFGMITAGELTD
                     YNKVFSLGFEF"
     gene            27418..30444
                     /locus_tag="PK015_02765"
     CDS             27418..30444
                     /locus_tag="PK015_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOL33428.1"
                     /translation="MLNKQSGKLLDKNKDNYNKLLVILVDFQEEIPDDPNTTGNGKFQ
                     LEPDPNYLYSIGSPPHNRKYFQDNLEALRYYYLAATAGSFNLEYDVYPLNGAYTLPHT
                     MGYYNPPSASSETFVSKMEEYFRDAFTTADNLSPEIDFSAYSHFMIIHAGSDWQHDIF
                     SDTPSDLPSFFISVGEGKEVPVDDGSFFISYACNVPSTISQDFTEEIYEGTVIHSGYG
                     ALNSVMAHEFGHSLGMVDLYNVYTWQPMVGVFDIMDSGGSGVLLDILEDGSYIMLEGG
                     LPVLPGAWSRTLICEDYLKSKGLLLDLEQIPLFDPILLSASSWKQSGNSYHPQILRIP
                     LSSTEYILVENRNVDPDGDGATAVFANEDKRVILYPTAIEDPDNRPTYEYDYLLPSFQ
                     KADGSAMGGGFLVWHINNDVIYNQGVWDNEGNFISNYQNNSVNTRYSRRGVEIIEADN
                     LPDIGYPYSWYWTGTQYEYFYAHKPLLDSDGYFIHWTQEPWKPVLNSNTKPALKDSQN
                     IGSLYWLNNMGNPSRDMSITVCAGNFETTQIFDYSDSRITCAPLIKSSFCDFNIPLIL
                     EDSIHLLSYSDNTWFDLMGSFPWNSHTIDFPITTSDQDGNGFQELVLVHNNTLELIQF
                     SQDDLESVSLNFSEHITTAPISFEDTIFVTTQNTLSSIHNNQVINSIPLQNVKRLGSF
                     DDKLIALCPDLLYIIDTINLTVSEEIELPEPFGNYEPVTFCALSENTSMLFLMSNKGN
                     IYRYEKGYLQQIYMARPGEHPTQIGLTCYRTGETSISPAIFWGSGNKIYAVTFDGSLL
                     SGYPYDISPFTIEPEAHLIAMQFNSSPLLYFPLSERGFLAFSPDNGILWQYSLLSNGE
                     TGNSHLMIIPDHQLSSHLYWYYTDGSGKIYIQGKNILTSETQLCWNGFRNGGNGTLYG
                     EFSISVPAQAFNAYIFPNPVKGDNFRVRIENFNQTVKLHLYNINADLIKSETVPANGN
                     LQRDIIIDSRKLSSGVYLMKVQCGNKHKTIKFAVQK"
     gene            30461..31459
                     /locus_tag="PK015_02770"
     CDS             30461..31459
                     /locus_tag="PK015_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33429.1"
                     /translation="MKKFLLIIICLGLLSTLLGQGIDDLIFQENKKDSEQEIIKVNFE
                     KKDARLAMLYSAVLPGAGQFYAKPSAVITYIFPILEIAMIGGMIYYDKQGDEKTKDYE
                     KYANGEIVTHTFNYNVGGVDYSYTYTGTRYRRDYQTQVQNVLKNINAFDIYDETFFRL
                     DQSDTQHFYEDIGKYNKYIFGWADWYHRFATDPTSGDGTFIMDDPAYEGLWVFTGSTD
                     PQLIQNRRWYQNYTVEDFMNGDLSHPISPKSPEASPWRQEYINMRKDANKQYDYSHYF
                     TLGLAANHIISAIDAYIMTNQVNRIAITKNDKVKMFYYTDVSNNSLTPTLALRVNF"
     gene            31464..32114
                     /locus_tag="PK015_02775"
     CDS             31464..32114
                     /locus_tag="PK015_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33430.1"
                     /translation="MMKHLVFLLLILSIMPLAALPKIIYSATSAILPGTGELALGKTT
                     QGAIMLSADILVWSSFFALEKEKDNLTDTYKQYAKVYAGAPENMPASYYQNLQNYISS
                     DDFNRYQELFARNYYLIYSYNPEGYEAYILANTYSDEEGWSWQNQTYQNHYKKLRRRT
                     QKAKIYQNLSVGALLLNRVVSVIDVSLTNKKAKEPLSIYFTPTSNNGLMFNVQLEY"
     gene            32114..>32758
                     /locus_tag="PK015_02780"
     CDS             32114..>32758
                     /locus_tag="PK015_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33431.1"
                     /translation="MLNKFKVTFFLILLLLLLTVSLSAESRGVKVLKSLVVPGLSQIQ
                     DGHNYGYAMLSAEAGIISTLFYFNEEEKLKAQKYYEYAIKFAHIEPGKYDDQYYRNLS
                     RYNSSGFEAGGYNAKIREEAMQLYPNDPVQQQAYIDEHCYPEEYGWNWDSLEHRRHYS
                     QLRIQARDLQDYGKLTMGVLIVNHLISGIDALRYTGKEINPQVYLDIKDNQPLLM"
BASE COUNT        11034 a         5737 c         6065 g         9922 t
ORIGIN      
        1 gattttatgg acctaatctt agaatataga gatttaagat atcaggaaca aagctatttt
       61 gcagaagctg aaaataaagc tgaaatcacc ttgaaagaaa aatataattc tctggtttct
      121 gcgaaggcta ctctattaag actttatgaa aattatataa aaaaacatcc ccattttgaa
      181 gcgttacttc aacccgatgg taactattta gtgagtaatg caatgttata ctataatatg
      241 gcagaactac aatatgctat agatttagac caccctgata aagctcttac ttattataaa
      301 aaagttctgg agatagaccc aaattattat aagaaagatt atgtgctata taatataggt
      361 tatttaagta gtgagagcat taaatccact ctggataatg ctattgaaaa ttatagaaag
      421 actcatccat acaaagaccg accgagcaat ttaaaataca ctgagcaagc ttttaaagaa
      481 gcaatagatg cctatactga attagtagat tcgggtctct ataccaattc tccactttat
      541 gatgaagcga tttatagatt gggtactatc tattttctca taggttcaga tgccgatgaa
      601 cctatcaact attataatga agctattcgt agatttgata ctttagttag caaaccagaa
      661 agtgattatt actaccattc tctacatcaa agaggatggg ttaagcttaa tttaggtgac
      721 gaagcttctc tgcagtcagc tatctttgat tttactaatt taattgaggc tgttgatatt
      781 ggagaaatag aagatcctta tttagcctct gattataaga ataattctat tgataacatt
      841 gcctatgcct tagttgcttt agatggaatt gattttatta acccggctaa aggaatacaa
      901 ttttttgaag agatactgat aaatcatcct gatactttag ttaagtctga aataatagat
      961 aaagctgttt ccttaaaaat ggaaataaat gctccactcc aggcaattga ttatcttgag
     1021 ttacgtctta aagtttttcc gctggattta cagaatccca gtattattga ttccattatc
     1081 actatttatc atactcccgg actggtttta cgagaaggaa acgacttaac taccatctgc
     1141 aatgcagaat atgattttat taaggcaaat tataataatc attcatcctg gtacgaacac
     1201 aacatcaaaa atgcaaaatt agataacctg gaaataagaa aacaattaga tattataaaa
     1261 agagcctatg agcaaattcg gattagatat tacaatcaga taattgactc tattgctgat
     1321 tcagatttac aaatttataa tcaacacctt caggaatata tagattatta cgaactcttt
     1381 aaggattcca ctgattttat tacctggcaa aaagaagttc aacgcacaaa tacgatttta
     1441 tatacttatc tggcagagaa acgtgatact ccagtggatt attatattgc tatccaaact
     1501 ctttactctt ataatgaaac taatcccgat aacccaaact atttaaataa tgaaggttta
     1561 gcctatagat atgccagaaa gatatatgat tatcttgaag aagaatttaa acatactaat
     1621 ctgaaaactg atccgctatt accaacaaat aaggatgagc tttacaattt ctatgaagct
     1681 gctgccttaa gattttataa tcatctaatt aatacagaag atgagggtct tagaaaaagt
     1741 gctattccta ttttgatgga ccttgcagct gtagctttag aaaatgggaa agattatatt
     1801 gctcaacaat attatcaaca actacttaat aatgcagacc aactggattc tcccactcta
     1861 agaaccattt atatcaatct ggctaaaata gctgaaaata atcttaatta tactgaagct
     1921 aaattgtatt atgaaaaggc taaagattat gcactaaatg ctcatgatag ccaagagctg
     1981 gaacatttaa ttagattgca acttcaaaac agttatgaag aagcagagcg caatggtgat
     2041 tacctcaaga tggctttaga cctggaagaa ttagcaaatc gttttatgga cgaccctgaa
     2101 caaagtaaag cctataaata tcaagcatcc gaagcttata agaaagctgg taattatcag
     2161 aaagccatag atataaaact gcaattagct aaaagtaaaa atagtattga agaaaaatac
     2221 tttttatatt atgaaagctg gaatatagca gattcgttga tgaaaaatca tagttacgcc
     2281 cgtcaattgc aggaagaatt tactgcaatg tatccttcaa gtaatcaagc atttaatcta
     2341 aaaatagcaa tgatagagga actgaaatcc aatcctgccc aaagagaaga tgcagctcaa
     2401 atgtatctcc aactttatca ggatgtccgc tccggcaaaa tagatagtgg caatattccg
     2461 cttgaggata tctatctctg ggcagtaaat atctacctgg aagaccaaaa tcaagataaa
     2521 gctttggaac tgctttccaa ttttataacc ctttacccca attataataa agccaccgat
     2581 attcttactt ttttagcaga tacctatttg gcaagaggtg aagaggataa atttgagtct
     2641 tatgcacgaa aactctatca gaaagataaa agccagtcag aaaggtatct tactattgca
     2701 caactaaaat taggcagatt agctcatgag tttgatgatg cctataaaaa caaagattgg
     2761 aacctggctt tccagaaacg tgatgaattt attaaattag aagcccaata tctaaaagat
     2821 ggtttaccca ttaacaatga agctgcttat gaagcttttg cctatgcaga ggcagaatat
     2881 aaaaatgaac aagcaagaaa agcttacctg gataattttg accgtcaact caaaaccatt
     2941 gcaaatggtg aactattaac aagctcagca aataaattaa taccagtaaa cactcaaact
     3001 acctggagag gttctttatt tgagaaatct ccacgatata ttccaagtct aaagaaaaga
     3061 gcagaaactg aatgtgctaa aattattcac ctgctggaac aacccgaagc agattttctg
     3121 gatatagacc gtcgtttgaa agccctgtgc ttaattgcca gaatcaatga acatacagct
     3181 gaggtgatag aaacacagtt agataaatat ttccagattt caaatgaaat ggctcctttt
     3241 agagaccgta aaaaatatag tgaagaagaa tataataatt tagtttacgg tcaattaatg
     3301 ccgtatgctc aacagtttat tgagcctctg aggacagctg caatcagcat ttatatggaa
     3361 atatacaata attacaatgt tgctggatgt gttaatgact acactaactt agctgaacta
     3421 aaacttctgg aacaaaattg tttgcctaat tattatgagg tagccttccc cttagaccaa
     3481 aactggaatt taaacttaca gcttccagat ggaaagttca aaaaagtcaa cccttataca
     3541 gaaagtatta tgtcgccaaa aggtatacaa cttttctcaa ttcaaattcc cgctcataat
     3601 gcattaataa tggaacggga atttactttt gaatatcctc ctgaatttgc tttcttgcac
     3661 attgtatatc ctgataatcc tgaaatatgg ttaaatgaaa atccaattga tttaatttac
     3721 ataccggttg atactttaat aactggtgac ctatctacca ttcattttgc cgctaaaata
     3781 aacaaacaag cttggaaaca gggttataat gaaattaaat atatcttttc taataatttt
     3841 gataaaccgc tttcttttta cttcaattcc aacttattct ttgatgctga tatgattgac
     3901 gaattcagtc ttttaagaac taaaagaata ataaccaaca ccaactggaa cgccttaatt
     3961 aaggataatg agtctgggaa agaaacaaag actaaaacca taaaagcttt tacctttgac
     4021 cttcctcttg ataggtctga attattgaca gatacatcgg ttaaacctat ttgggtggaa
     4081 gaaaactctg atcatcccta taataatatt actttcgaga ctgaatttat acttgatgaa
     4141 gaggtgcatt atgcttatat agatttggtt gctccagaaa ttgctactat ctatataaat
     4201 gggacaaaaa tagtacagaa attgcctctg aattttgatt ctttcccttt tatggtgtat
     4261 ccttcaagaa ttgaaattcc tccagaagtg cttagaaaag gtaaaaacat cttacgaatt
     4321 gaaatactga ataattctcc tttccgggga atgatggctg aaatgaattt taacctatca
     4381 actacaggag aataaataat gaaccgcttt tggtttattt gttgcatgct tatctttatg
     4441 atgcctttat gttttgctca ggtaaaaagt attgcatctc cagcttcaaa cgatggagaa
     4501 ataataaagg aattggaaat tcagattaag actatgttac cagatatcaa tatagtgcgt
     4561 ttaaaaactg atacctatat ttccgctgga caatataatt atactaaata taaggacctt
     4621 attaaggcag aaaatattaa aaatcttatt aaattacaaa aagatataac aaaacaacca
     4681 gaggagtcaa aatgaaattt aaacacatct tactcgcatc attaatatta attattggta
     4741 ttggtccttt attagcccag accgctcaga acccatataa tatacctcca ggaactgaaa
     4801 agattactct tgcttctatc ttcagagata gcaatgctca tggtatattc ggatatttga
     4861 ttctactgac tttcaccata ggtattgttt acgctattat ccgctatatc cagttatatc
     4921 agaaggaaaa agtcaatgct ccaaatctat ataaaagtct aaaaggttac attaaaaaca
     4981 accagattga ggaagcaata aagataacag aaaaactgaa agataccact cttggctaca
     5041 ttttttggaa tggaattact gcctataagg aaattcctaa ggatgcttct aaggaagagg
     5101 tatcaactaa tgttcaaaat gcctttgacg aagctgctct ccaaaaagtc tataaacttg
     5161 atgcaggact attctggttt gatattcttg ctcaagtatg cacttatctc ggattattag
     5221 gtaccatctg gggtctactt tatgcttttg ctgctatcag taatcccacc gttctggata
     5281 ctcaaaaaaa tcttatgtta tctgctggaa taaagaccgc tatcggaaca acagctttag
     5341 gattaattgc tgctatacct ttaactctga ttaagggtgc attacaaagt agagctcaaa
     5401 aaattattaa tgatatagat gaattcagtg ttaaatttat taatttcatc acttctttag
     5461 caaaaggata atattatggc tcatcctcct tctgcccgaa gggatcagca tcatttttca
     5521 ccacctaaag tgtcagattt ggtgcctttg atgaacctgt ttctaattat aatacccttc
     5581 ctcctactaa tgatggtggt tacacaaatt gctatgatag cattgaattt cactcctgga
     5641 actggaacag agaaacaagc gggaggacct ttaaatggca cttcggtaac gcagaaaaat
     5701 atacccgaaa ttaccataat tattatggct tctcaagatt cgttaggaac aaccttccct
     5761 ggctttgaaa tccgagaacc ggaaaaaaca aaggataagg ttggtttagt taatggctat
     5821 tatgattatg taaaattaga tgagaagctt aaggctatta ggcaaagata tccagacttg
     5881 caggatatca gtgttgctcc ttatgatgat gttttatatg atactcttat taagactatt
     5941 gacctttgca gggaaaataa cttctccaat atacactata aagctcctca ggtcagatac
     6001 tttgctcggg aggttaaatg aatcagtcaa agtattcgcg tgaccttaaa cacctggtcc
     6061 ctcaaagaca taaagatgaa tggagcttat ctatcatatc tctgattgat attctaacta
     6121 ttttattagt ctttttactg gctaatgtaa cggtggaagg acaaaaattt actcaaccca
     6181 atcggatgac ttttcccgta agtatgaaaa aacgagattt gttggagaaa aaaggaacaa
     6241 cagtagttca aatctatcca gatagaatct taattggaga gaaagggata tattttggaa
     6301 cactcaatga attcgctaat gacccagtaa aaagaacaga aatattgaaa tatttacaaa
     6361 ccacagctct acaaattctg gaggataaag atgaatccgg aaatcccaga actccgccaa
     6421 ctcttcttat ccaggctgat agagccatac cttgctggta tattacagaa tttgttagtt
     6481 taggcaccag cgcttattac gaatatatct attttgctac tcttttggat gctaattggt
     6541 ttcaacattc tgtattatca accagaaggt agagggagat ataatggtaa acaaaatttt
     6601 aactttaaaa ctcttttata agggaaagct tctggatatt atagagcaag aaaaagactt
     6661 cactaataaa tggtatatag gaagcagtaa atacctttcc tggcaaattc tggatgaatc
     6721 taacaagttt cctcctaaat ataaaatact gatcaaaaag ggtggacatt attatctgaa
     6781 tcttcctcaa ggctccactt taacctgccg gaaaaatgat agtcccgtag atgctaattt
     6841 cttaaaacaa aatggtatat tggtagattc tttattaaaa ctccgtgaag atatgactgg
     6901 tattattcag gttcatcctg actattctat tcaatatgag ttcattgaac ctgttaagat
     6961 tatattgact cctcaggaaa aggaagtaat aaaacaaacg gcacgtcctg ctcctcttgc
     7021 tcctatggat aaaacaacca gagtggtagt tattttggca ctgatggcag gaattgcatt
     7081 tagtattttc tacgatttga aattaaaacc agaaatgttt agagagcaga ctctttctga
     7141 actattagct gaaatagaga aaatcaggga aataaagcca gaaatttctc aatctcaaac
     7201 cccatcaact cctttaacta ctttaccaga agaaccagct acttcagaaa aaactactaa
     7261 aaatgcccag gttggaaaag gtaagagcac tggaaaaggt ggttcatcaa caccctctgg
     7321 cactcaaagt atctttgggg accttggaac aagctcgggt acttatgctg gaggttccac
     7381 gcaatctgtg gttggtgcag ctgtgctttc tggttttgtt accgcaagac cgggtgttaa
     7441 aggaggaagc ggtattggaa atgcttccgg cacttttcaa ccaagtgctg ctgctggttt
     7501 gggcagtggt ggctcttttg atcctaacgc cataaatcgc tttaatgcag gggatgttcc
     7561 ttcggttgta agtggaactg ctccagaagg aggttccacc gtaaaacctt ctggttcagc
     7621 tcaatattac actggtcctg cagaaaaact aaaacctttg gctggcggtt ctttaacttc
     7681 ggttccggat gttcgtgcag aaacagaaaa aatagtcagc caatcttata aagcttctga
     7741 acctgctaaa attcaggaac ctgctgtaac catagttaaa ccatctacta ttgttgctgg
     7801 tggagatatc atatttaacc aaattcggtc tcgtaaggga cagatagagc aggcttataa
     7861 aagaaatgca gccatcaaaa aacagagtgg ttctataact gtaattcttg atattgcttc
     7921 tgacggaaca gtcaaagcta ccatcattcc aaatagtgtt acttttactg aaagtttcct
     7981 gaacgagatt aagtccatta ttgaaagttg gacatttaat gtcagtcagc caaccactta
     8041 taaatttaaa atgaatctca gtcaaggtta gagcattttt taaaatgtat agtctatacc
     8101 tatttgatat tagttttaac aagaaataca aaaaactata ttaaaggctg attataaata
     8161 taaataggaa agctactcac tttcttatct cctatttagc tgcaacatat acaataaagc
     8221 aagcaaaagg caggaaagag gtaagtacga gtagtgcgaa actataattt tacattcttt
     8281 aattatttga atctaatcac aataaaaaag atttgacaaa aaactcttta tttaaaattt
     8341 tggatttaaa gaagctgctt gcttctgcct taatgcgaaa gtgcaggtaa ggctcaataa
     8401 aataaaatat atataatacc agaaagcagg cggatttctt ccgccttttt tcttttccgg
     8461 taaaaggagg ttcttataat ccggattaaa cgtggaaagg cacgcacaaa tgaagtggtg
     8521 cccaaggaaa gaattaatga acaaattacg gctaacaaag ttagattgat tggtgcggat
     8581 ggaaaacaaa ttggaatagt aagtctaaaa gaagctttgc accgtgcaga agctgaagat
     8641 ttagatttag tagaaatatc tcctaatgct gatccaccag tatgcagaat aatggatttt
     8701 agcaagtact actttgaaaa agagaaaaaa gcaaaagaag cacgaaaaag acagcatgaa
     8761 gtagagatta aagaaatcaa attgggacct aatacagaag agcatgatta caacttcaaa
     8821 aagaataatg ctattaaatt ccttaaacag cataataagg tcaaatttac catccgtttt
     8881 cgtggtcgtc agatggcaca tcaagaactt ggattgaatg tattagagcg aataaaagca
     8941 gatttaggac atattgcaga accggaaaca gaaccggtag cagaacggaa tatgctttac
     9001 attattatgt ctcccaagaa agatattgat cgtattttgg caaaaatgga gaaagaaaaa
     9061 gctgctttag agggaaagac ggtcccaact acccaagaca aagctaattc ttcaaccccg
     9121 gctgaaccta aaaaggaaaa cctaaaataa gttacatcaa aaccaattga tttagaaatt
     9181 aattaaggag ataaagatgc ctaaaatcaa aaccagcagc tctgctgcta aaaggtttaa
     9241 agttactggc acgggcaaaa ttttgcgtca tcatgccaaa agttctcata ttaaaactaa
     9301 gaaatctccc aagctaaaac gccatctgcg ttcagttacc ctggtcagta aagctgatga
     9361 aaaaagagtc aaaagaatgc tgggaattta agggaggaat aaaatgccaa gagcaacaaa
     9421 taatgtcgca gctcatagaa gaagaaaaaa atacttaaaa gctgcaagag gaaattacgg
     9481 tagacggagt aatacttata gaatagcccg acaaactgtg gagcgtggat taatcttctc
     9541 ttttgctcat agaaaacaga aaaaaagaga gttccgtcgc ctctggatta ctcgtattaa
     9601 tgctgcctgt agaaacaacg atatgactta tagcaagttc ataaatggct tgcataaagc
     9661 caatattgcc ataaatcgca agacccttgc ctatcttgcc tggcatgata gcgaagcttt
     9721 tgccagactt gttgaaatag ccaaagaagc acagtaatcc aaccccctaa aggataaaga
     9781 agttgatgat agatgaccag ctaaagaata tagctgaaca agcaaaaatt gaccttaaag
     9841 aatgtcttaa tcagaacgat atcctgaata ttagagccaa atatttaggg aaaaagagtc
     9901 tggtaaactc tctttattct caattacgta atttgcctga agaagagaga ccctctttcg
     9961 gcaaaaaggt caatgagctc cgtaatgaat tggaaagaat gattgatgaa aaagaaaaag
    10021 aattaaaatt gcaaattgtt aatgcctatg attctgttca agctaatgac ctaactttac
    10081 ctggtgtcta ttttcctcca ggttctttac atcctttaac cattatccga agaaaaatgg
    10141 aagatatttt tataagttta ggattccaga ttgcagaggg tccagatatt gaagatgaat
    10201 atcataattt tgatgcttta aatacacccg aagaccatcc ttcaaggaat ctcgctgata
    10261 ccttctatct ggataatggt tatcttttgc gcactcaaac ctctaccgta caagtaagaa
    10321 ctatggaaag ttaccctcct ccaatctaca taatctcgcc tggacgttgt tatcgtaatg
    10381 acaaaccaga tccttcgcat tctcccgttt ttcaccaggt agaagggttg gtagtggata
    10441 aaggcatctc aatggctgac ttgaaggaca ctcttcaatt ttttgctaaa gctatgtttg
    10501 gaccggaagt caaatccagg atacgtcctc atttctttcc tttcaccgaa ccaagtgcag
    10561 aaatggatat aagttgtgtt agttgtcagg gcaaaggatg cagaatatgc aaaaactctg
    10621 gttggttaga attagctgga gcaggtatgg ttgaccctag tgttttagaa atgatgggta
    10681 ttgatgacca gatatataat ggttatgcct ttggcatagg cattgagaga attgctatgc
    10741 ttaaatataa cattcctgat atgcggattc tctttgagaa tgatgttcgg atgcttcgcc
    10801 aattcagtag agaattaata tgattgtatc cactcgctgg ctcagtaaat acattgattt
    10861 gcctgaaact ttagaggagt tagtccagac tttaaccttt acgggaatag aagttgaagc
    10921 aatacaagaa atctccgctt tgccggaaac tgtagttacc gctcgtattc tcaattcaga
    10981 aaaaattccc ggaactgaac atcttcaagt atgtataata gattatggaa aagaacctct
    11041 ccaggttgta tgtggtgctc ccaattgtcg tagcggatta atcactgctt ttgctcaagt
    11101 gggttctaaa ctgccagaaa tggagattaa aaaaaccatt atccatagtg tagaatctgc
    11161 tggtatgctt tgctctgaaa aagagttagg aatctccgat aatcattctg gaattataga
    11221 actccctcct gaaactcctg tgggaattcc aataaatgaa atctatgagc tcccagataa
    11281 cattttagaa ctggagataa ctcctaatcg tccagacctc cttggttata ttggaattgc
    11341 tcgagacctg gctgcttctc tgaataaaag gttaaaattg ccatctataa ataactatcc
    11401 tggcgaagaa gattcttctc tacatttgac tttgattctt caggaacccc aaaaatgtcc
    11461 tcgttatacg gcaagaatca tagatggcat aagcgtcaaa gaatctcccc tatggctaaa
    11521 aaatgctctt atcaaatctg gtctgagacc aatcaacaac attgtggaca taacgaatta
    11581 tgttatgctg gaaacaggtc aacctttaca tgcatttgat tatcataaat tgctacctat
    11641 taatcagcaa gagtcgcatc cagctataat agttcgtaga gcaaaagctg ctgaggaatt
    11701 tattgccctg gatggaaata agtaccttct ggacgaagaa gatctcgtca ttgctgatgg
    11761 tcaaagacct tccgctttag caggaataat tggaggaaaa gatactgcca tcagcaatca
    11821 tactacaacc attgtccttg aatcagctaa tttctctcct tctaccatac gtaaatcttc
    11881 tttaaagcac aaaattagca cggattcctc ctatcgtttt gaaagacatc tcagtccagc
    11941 ctatcttgaa atggtaagca atagagcctt agaacttatt attaatgttg ctggaggaag
    12001 agtttgtaat gaactttatg acgcttatcc tgtacctcaa aaacccctct ttcttgctgt
    12061 gcgtccttct cgttttgaag aacttatcgg ttacaaactg gatgcctctc ttataaataa
    12121 ttatcttcaa tccttaggat ttcaatatat cctgtgtgga ctctggcaaa aagaacctat
    12181 taatgaatat aagcttttgc ctcaagctat ggagctggat ttagaaacca gtgataataa
    12241 gattttttat gaaaatcctc aactggctct atattttctg gtgccctctt atcgggtgga
    12301 cataactcgt gaagcagata taattgaaga actggcacgt ctggatggtt ttgataagat
    12361 tcctcaaaaa aagcttattc atcccattat ggattggcat gctcatcata ttaaacgtaa
    12421 aattgaagat tactttcgtc aatccgggtt ctatgagatg attaatccaa gcttcattga
    12481 ccctgtcaaa ttagaatatt taggagaaga taaagcagaa ttagagaaaa ggctgataag
    12541 aatcgtcaat ccacaaagca gcaatcaatc tgctatgcgt accactatgc ttccacaatt
    12601 actggataat cttctctaca atcttaatca tagtgaacgc aatttaaaat tgatggaaat
    12661 gggcaaatta tactggaaag atggtaataa aaattgcgag actttacatt tgaccgctct
    12721 aatgacagga ttgaataatt tagaccactg gaaagtaaag aatgaaccta ttgatttata
    12781 taatgtcaaa ggagtagttg aagggttatt agaccagtta agtatcacaa ttgatgaaac
    12841 gaaaaatgtt catcatccct ggctggttga atcagataat cttgcctatt tttgcaataa
    12901 ccatctctgt gcttcctttg gaagattaaa agctaagact gctgagagat ttaatattga
    12961 tattatcact ttaaaacagg atatatgggt cttagatttt gaggttaata cattgataga
    13021 gctcaccaga accaataaaa ttgaattttc actactacca aagtttcctg cggttataag
    13081 agatatctct tttttaatta atttttctgt tccttatgca gatattgtga agtccataaa
    13141 actggttgaa ccctatctaa tccaggatgt gaaaatcttt gatgaatacc gaggaaaagg
    13201 tgtcccagat ggctggcaca gtatttcttt acatataatt cttcaggatt atgaaaaaac
    13261 attgacagag gaacgagtag gacaagtgat tgattcggta ataaaaatgc tggaaaatac
    13321 ctggcagata aaaatgaggt aagtctggat ggaaagtaat gtaataaatt tacaggaaaa
    13381 gatacaaaaa cttattgacc aatatacggc tgatagaaaa agattagaac agctggaaga
    13441 ggaaaataat aatcttcgtg aagaaaaccg tcagcttatg gaacaaattg aaaacactaa
    13501 aaagttccat agtggctctg aattaaaaat taaagaactg gaaaatcagg taaagcttct
    13561 gcaagcgcaa tataaggaac ttcaggattc tctggcagga ttagaagaaa ttgcatctac
    13621 tactataaat cagatagacc agctgatacc tgatttagat aataagaaat aataatgcag
    13681 tctgtggaag tagagatttt tggacgtaaa ttccggtttc gtactgatga gccggaacaa
    13741 actcaagaaa tagcctctac gattaatgaa gaattaaata ctctacgaga actttacgaa
    13801 aatcttgact ttacaagact gttactttta cttgcactta gaaagcaaga tgagattatt
    13861 gccctgcgaa aacagaatct ggaactggaa aagaatctgg aacgagtaaa tcaaatggta
    13921 gaaaaaatcg taggggaaat ataaattatg gcctgcggaa ctcgtgatat gggttgcgga
    13981 aaagctaata aaattaaggg aaccgagcca tgggtggcat gcatgccggc tagtcccgga
    14041 tccataaaac ccataaggta ggtgcccacc tagtgcttgg ctttaacgga caagcataca
    14101 cggttctcgc gggtcattta tataaacagt ggggttaacc cacctgaatt gaataaagga
    14161 ataatataat gcaatgggat aaagttattt ggttagccat cggagtggct ggaggacttt
    14221 taatagcact tgttatttat ctggcaaagc gtagcagctc tttccgtatt atctctcaag
    14281 caaaaaaaat agctgaagaa atcaaagaat ctgctcataa agagattgaa aatgcaaaaa
    14341 aagccgctgt cctggaagct aaagatgaat ggttcaaaca aaaaaaggtt atggaagatg
    14401 aactaaaaga gcgtcaaaaa gagcttagag atcaggaaag aaaatataat gaacgactta
    14461 gcaatctgga taaacgcctt gatgccttag ataaaaaaga aaacaatctt ctcggacagg
    14521 aaaagaaact taaatataaa gaagaagaaa tcaatcgtaa ataccaggaa tgtgaacaat
    14581 tcatcaacgc tcagaaagca aaactggaag aaattgcagg aatgagtaga gaacaagcct
    14641 tgcaaatgat gcgagaggaa ctaatcacgc aagctcgtca ggtggctgca aatgatgcaa
    14701 aggctactat agaacagatt aaactggatg ccacaaaaaa agcttccgaa attctttcta
    14761 ctgctattca gcgtcttgcc gttgattatg tatctgaaac tacggtttcg gtagttcctc
    14821 ttccttcaga tgaaatgaaa ggcagaatta taggtcgtga agggcgaaat atccgtactt
    14881 ttgaaaaagc ttctggagtt gatttaatca ttgatgatac cccggaggct gtagttcttt
    14941 cctgctttga tcctgtaaga agagaaattg cacgcctttc cctggaaaaa cttattgctg
    15001 atggaagaat acatccaggc agaattgaag aagtcatctc caaaacgagc aaagaaatgg
    15061 aagagactct cataaaaatt ggagaaaaag ctgttctgga taacaatatc cataatattt
    15121 cagcaaatct aattaagatt ctcgggcgct tgaattatcg cacttcttat ggtcagaatg
    15181 ttcttaagca ttcaatggaa actgcctgga tttgtggaat gctggctgca gaactgggat
    15241 tagaccagga aatagctcgt agagctggtc ttcttcatga catcggcaaa gctatagacc
    15301 aggaatttga aggaactcat gccattatcg gtgcaaatat agctcgcaag aatggtgaaa
    15361 gcaaaattat agttaatgct attgaagccc atcacgaaga agttgaacca atttccgtct
    15421 atgcagcttt agtgcaggct gcagatgcaa tatctggtgc tcgtccagga gctcgcagag
    15481 aaatgctgga gacctatatg cagcgcttaa ctaaactgga agaaatagct aattccgttc
    15541 aaggtgtaaa taaatcctat gctatccaag caggaagaga attacgaatt attgtagaac
    15601 ccaccttggt tgaggatgct caggcagcac tcttagccac agaaattgcc gctcaaattg
    15661 aaaaagaagt tcagtatcca ggtcaaataa aagtaacagt aattcgggag acccgtcaga
    15721 ttgctatggc aaaatgatga aagtgctctt ttttggtgat gtgttcggta aagcaggcag
    15781 aagaatagtt tgcgattatc tttctgaact aaaagaagaa tttaatccag attttgtcat
    15841 ccttaacggt gaaaatctcg ctgatgggag aggtttaact gaaaaaactt ctaaggcatt
    15901 gttcaatgca ggagtagatg ccatcactgg tggaaatcat ctctgggata gagctgaaag
    15961 ctgggattat attaaaaata atcctgccat aatcaaacct ttgaattatc ctgcttcagc
    16021 accaggaaac ccttcttata ctatagtcaa aaatgataaa aagctcacta ttataaatct
    16081 ttgtggtcag ctttttatgc ctccaactaa ttcacctttt atagcatttg aaacctggtt
    16141 aaattcatct tatatttata attcctcctg tatcctgatt gattttcact ctgaatctac
    16201 ttccgaaaaa cgtgcatttg gttggtttgt tgatggaaag gtctcagctg ttattggaac
    16261 gcatacgcat attcaaactg cagatgaaga aatactccca caaggaactg cttacatcac
    16321 tgacgcagga atgactggag cccataacag tgtcattggg gtaaaaaagg aaattattat
    16381 tgagaagttt aagcattgtc ttcctctaag atatgaatct tctgatcttg gtccgcagat
    16441 aaatgctgtc tatttagagc tggataattt aacccataaa gctcaaaaga tattgcggat
    16501 tagacgtcct attgagtata aaaactccat ctttaatgag gaataatact ttggaaaaaa
    16561 tattatctgg aaagccatta gctaaactta ttcaatcaaa aattaaattc ttaatcgctc
    16621 aagagaatct acagcctaaa atggctttaa tacagattgg agaggatcct gcttcttcct
    16681 actatgtaca aaatatcatt aatatcggga ataaattagg ctgtgaagtc ttatataatc
    16741 aatatccagc tgatttcacc caagaagatt tattgactat actggatgat gctaatacag
    16801 acccttcaat taatggaatt atgattcaaa aaccattacc tccttctttt gatgaattga
    16861 aaatagctga gtccattaac cctaaaaaag atatagattg tttccatcct gtcaatctgg
    16921 gacgcattat attagaagaa gatggattgt ttccttgcac tcctctggct gttatatgca
    16981 ctttaagata ttataatata gagactcaag gaagtcacat tgttattatt gggcgaagta
    17041 atattgtggg caaaccactg tcaaatatgc tactctggaa aaaaccttat gccaatgcta
    17101 ccgtgactgt atgtcatagc agaagtaaaa atataaaaga actaactcgt tctgcggata
    17161 ttctaattgc agctctggga agaccttctt ttgttactga agatatgatt aaagcagaga
    17221 gtgtcttgat tgatgttgga atcaatcagg ttacagataa ggacggtaag caaagctatg
    17281 ttggagatat agattataat acttgctttg ataaagctgc tgcaataact cctgtacctg
    17341 gcggaattgg ttccgtaacc tcttcccttc tttttttaaa tttacttaaa gcttgtttta
    17401 cagctcataa cattaataaa tctattgacg attttcttaa ttacattttt gttgaataaa
    17461 tgaatattat aagccaaaca cccgtgggag gtatgatgct aacgaagagt aaaagactct
    17521 taataatgac catatgggtt tctttaattc tgcttgtctt aactggttgt ggtaagtccg
    17581 gtcataaatt tgctaatcaa gaacctacta tccgtataac ttcttatgag ggttatgatg
    17641 attctgagtt gctggcacat tatgcggatt ctctttttat ctttcagcaa aagattttct
    17701 ggagtgcgag tgatcctgat ggagtaatta ctggatatgc ttatcgggtt aaagatcaaa
    17761 atgataatcc catatctact cctggtaatg aatacattga tttaaatggt gatgttactc
    17821 ctgaaaatgt gattgaaaga ttcggaactg gttgggtgct gcactataag cctaacgcaa
    17881 atcaagatct tcccttaaat gaccccaatt caaggagaac tatctggacc tcagcaaaat
    17941 atgcagtaat taacttccct gctgcagatg aatttggcaa tcctataaca aaagaaagta
    18001 cttttgaggt gattgcaatt gataatagag ggggtataac tccagttgat ccatcatacc
    18061 ataaaagaag tattgcctgg cgaaatttta aatcaacttc cgcaagacct acctgcttct
    18121 taagtactac caaaggcaat cccaatggtg aagaagtcgg ttctggaatt cgtttatctt
    18181 ttacaatgaa agatgaactt aatccttctg ttcctgctat accctataaa tttgaattta
    18241 aaataataaa acttcaacct gatagtacta ctattattag tgaaagcgat tggtatgata
    18301 cttctatctc tcccaatgat accaatataa ataaattcct tttaaccaga tataccactc
    18361 cttctttgag ttacgacatt gaaaatgggg ttactcaatc aataacgaaa gtagtagctc
    18421 gtacctacaa tatggcagga gttacttctg aaacggatgg ttattcttca atttcattta
    18481 aagttaaagc tggattccga cctaagactt taatatataa caaacgttgt tatgctctgg
    18541 gacaaaatca ttatgttgat tattatgatg aatcttctgc agaagttatg ccatttacca
    18601 taacttcagg cacgcaacgt tatgccaccc atttctttgt tgatttagat tcaactctga
    18661 ctgccataaa tagcaataat attaaagtct ggttgcgttg gggatggcat ggtgaatatg
    18721 ggaaaatact cgcttccggt gattatgtag ttaatgatga tccttatgaa agaaaaattg
    18781 actatctact ggacagattt actgataaaa actattattc tgaaattact cattttgatt
    18841 tgagattgga tggccaaccc tataactatc ctcctcttgc taacgatcca aacgctcaca
    18901 ttgtggatga ggatggtaaa gcctggataa gattacctct atattctcca gtaagacaaa
    18961 ctttagttct aacctcattg tccagtggac tgcatacatt tgaagttaga tgtgttgacc
    19021 ttcaaggtga agttgacccc attcccgcag tatttacatt cctcctggta gacaatcttc
    19081 ccattgcaaa tcgtaatggt attttgatta tagatgatga tcctaatcat ccatcggttg
    19141 caccagatgc tattatccga gctaaatatg aagaaatgct ggctgattat aatggaccaa
    19201 aagatttcat aaaaagaaaa accgatatag agccaggtga tacccatcaa gacctaagtt
    19261 taaggcatct tgcaccttcg gatttacaaa aatataaact ggttatctat cataatgata
    19321 acttccagag ttcaggtaac ttaaagcttg aaaatgatgg tttaactcta tatcttagat
    19381 ctggtggaaa tctaatcatt tcatcaacta atacattagc acaaaatctg gatgctttcg
    19441 tcttagctaa acaaaagact ataattagtt ttctgggtgt atattatgaa tctccaccgg
    19501 caagtatttt gagcaatgct ttaacaatta ggcctttctt ccagcaagca ctcggacaaa
    19561 atggatatcc taatgtgaat cttcagtata gtgcagcaaa tgaaccaagc ttcgtgaatt
    19621 tgataaataa ttatcaaggc ttagctacta taacctattt ccccaaaatt agtgtcaatg
    19681 ctactgctat ttataaaatg ggaataaagc ctgttggata tacaccaaat ggacctactc
    19741 cagaacaatt taatacctat aacaatcaaa ttatcggaat acgctgtgtg accggaaatt
    19801 ctcattgtta cacattcgga ttccctctgt catatatgta tacaccagat gtaaaaacaa
    19861 tgatgaataa aatcttatct gaagtaatgt aacataatat cccccatcct ctaaataacc
    19921 ggcttgctta ctaaaaacga gccggtttat tttatctata aattcagttc ctgatttata
    19981 aaatagatgt gtttttctaa ataataacgc taactaactg tttttcagac tgttacacgc
    20041 aatattctct cttagctcct acttgcttct ctcttaacac tttacaactc taagagctaa
    20101 gggagaggca ggatactctt agactaattt aagaggtaaa ttatagaaaa aaatttataa
    20161 ttttaataaa ataagttaag aaatatcacc tgaatgaatt gcatttccct ccaactcaaa
    20221 tttgtaaatt aaagatggta aatcctgcat atatccgact tgttctctct ttgcttatcc
    20281 ctcacaaaac taaattgtac gcaaataaac ctactttcta tattgaaaaa aaataattct
    20341 tgcctaagca taactcccca tcaataatat tactgcgcat ttaacttgac aaaaaacact
    20401 tattgagaag aaagataaag ttataattct gattaaagct ttgaatgaaa caaaaatata
    20461 taatctaaat cagctgacag atagaataaa attttatcag aacctttagt aaagaggtaa
    20521 actatgtttc aaggtaacag atttgaggta aaactggatg cagaatttcc accagatcca
    20581 gtttttgacc ctcaatatag acgtgctcct catagaggat taaatttaac tccatctgaa
    20641 ataaccttag cagttaaaaa tgctttgcgt tatgttcctt ccgaatggca ctctcgttta
    20701 gcaccagaat ttctggaaga attattgacc agaggaagaa tttatgccta tcgttttcgt
    20761 cctcaaggaa gaatttgggg acgccctatt gacgagtata agggcatcac tccagctcgt
    20821 ggtattcagg tgatgattga taacaatttg gattttgagg ttgctctcta tccttatgaa
    20881 ctggttacct atggtgaaag tggacaggtt tgtcagaact ggatgcaata tcgtcttatc
    20941 aaaaaatatt tagaaataat gacagaagag caaacgctgg tggttcaatc cggtcatccc
    21001 ttaggtctat ttccttcccg taaagaagct ccaagagtta tttctaccaa cggtattgtc
    21061 ataggaaaat gggataatcc tgaagatttt caaagactta ctgctcttgg tgtagctaat
    21121 tacggacaga tgactgcggg tggatggatg tatattggac cgcaaggtat tgttcatggc
    21181 acctacataa ctctactaaa tgctggaaga aaatatttag gtattccttc ggataaagac
    21241 ctacgaggag tgctctttat atcttctggt ttaggtggaa tgagtggagc tcaacctaag
    21301 gcagtagaaa ttgctaaagg gataggtgta attgctgaag tagattatag tcggatagaa
    21361 accagattaa aacaaggctg ggtaaagaaa gtttccagta atctggagga aatctttcag
    21421 tgggtagatg aacatcgcaa gactaaagaa cctatatcta ttgcttacta tggaaacatt
    21481 gttgaccttc tggaatatgt tgattcgcat aatatcaaag ccgaattact ttctgatcag
    21541 acctcctgtc acgctgttta tgaaggtggc tacactccag ttggtttaac ttttgaagaa
    21601 ggaagagctt tgcttgcatc taatccggaa gaatttcgta gatgcgtgga tacttcttta
    21661 agacgccatt tccaagttat caaaagactt tgtgataaag gtgcaaaatt ctgggattat
    21721 ggaaatagtt ttatggcttc agtttttgat gctggagaac ccagtattgc tcgtaatggt
    21781 atagatacca gtgaaggttt catctttcct tcctatgtag aagatattat gggacctctt
    21841 tgctttgatt ttggttatgg accctataga tgggtgtgtc ttagtggaaa agatagtgat
    21901 ttacatttaa ctgaccaggt tgctgctgaa atcattgacc ctaatagaaa cactatggat
    21961 aaagataatc attactggat aacccatgca gaagaaaata atctggtagt gggaactaaa
    22021 gcacgcattc tttatgctga tgaagaagaa agaattcgca ttgctttacg ctttaatcag
    22081 cttattagag aaggcaaact tggtcctgtt atgataggaa gagaccatca tgataccggt
    22141 ggcacagatt ctcccttccg tgagacagca aatatcaagg acggaagtaa tagaatggca
    22201 gatatggctg ctcattgttt tgctggcaat gcagctcgtg gtttgactat ggtagttctt
    22261 tccaatggtg gaggtgtcgg tgtcggaaaa gctttcaata gcggaaatgg aattgttctt
    22321 gatggcagtg aatatataga taatgttatt aaatctggct tatcctggga tgttatggga
    22381 ggagtaggaa gaagaaactg ggcacgcaat gaaaatgcaa tgaagactgt ccgaacatgg
    22441 aatgaagttc attctgagga aggacacatt acaatgcccg aaaatattga tgacgaaatg
    22501 gtaaatgaga ttgtgcaaaa aagactgcaa aaataaaaat tactgaggat atattgatgt
    22561 taaaccagtt tatccgaatg atgaaggcag agtccattcc tgaaagtgtg attcgcactt
    22621 tctgtattta ttataatcaa ttggtaaaag gagaaagggg tatcattcct tcttctgaaa
    22681 tcaaccctcc caaagaagac aacctgcttg aatattcaga tctaaatgat tatagaaaac
    22741 aatctatgct aaaacagatt gtggtgatta aactgaatgg cggtctggga acaagtatgg
    22801 gattaagtgc agcaaaaagt cttctaccag ttaaaggcaa tatgaatttc ctggatatta
    22861 tcgtccgtca aattctatta ttacgtgtta ctactggtta tgatgtgact ttaatgttta
    22921 tgaacagttt tcacactgat gaggatacta aaaaatatct gcaaaaatac ccagatttgg
    22981 agaaacaatc acttcccgtt acttttcagc agaataaatt tcctcgcatt cggcaagaca
    23041 atctaaaacc ctacgataat cctgacccta aaaaaagatg gaatcctcct ggacatggag
    23101 atttatatac gactttatat tgcactggac ttttggacaa aatgattgat gcaggattcc
    23161 gctatatctt tgtgtccaat tcagataacc tgggagctac aattgatcca gccattcctt
    23221 cttatatggc agcaaatgac cttcattttg tgatggaagt atgccataga accgaaatgg
    23281 ataaaaaagg gggacatcta tgtgaagata aaaatggaca attgatgctc agagaaattg
    23341 ctcaatgtcc agaagatgaa ctttctgagt tccaaaatat aggactttat aaatatttta
    23401 atactaataa tctctggata gacctcaaag cattgcagtg gtatttattg actgaggatg
    23461 ggaaaatatt attaccacta attgtaaatc ctaaggtggt agatgggact cctatctatc
    23521 aactggaaac ggcaatgggt gctgctatca gtctttttaa aaattctcgt gctgttgtgg
    23581 taccccgaac tcgtttctct ccagtaaaaa agaataatga cttacttgct atctggtctg
    23641 atttatacga attaaacgac cagtatcata tagttctgaa acagggaatt gaacaggtcc
    23701 ctaccataga gcttgatgaa agatattatg gaaatataaa ccaaatgatg gaacatttta
    23761 aagatggagt cccttctcta attcgttgta aggaattaaa aattactggc gatataagtt
    23821 ttggagaaga tgttatctgc gaaggaaaag tgagtcttta tgctgaccat ccagtttata
    23881 ttaaaagcaa gatattaacg ggtgaaatcg tttttaatta aaaaatgata aattctatac
    23941 cgcatttagc tgacctaaag ctctctttaa ttgtagcaat ggaccgtaat ggtttaattg
    24001 gatatcagaa tcatataccc tggcgtatac ctgaagacct ggcatatttc cgtgaaaaga
    24061 ccttaaatca caccgtgatt atgggaaaaa atacctggat gtctctgggt agcactttag
    24121 atcaaagggt aaacataatt cttactcatg accataactt ctatgttcca ggagcaatag
    24181 tttgtcatag cgtcagtgaa tgtctggaac attgcagacc tgatgatgaa gtctttgtta
    24241 ttggaggaag tcagatattc aaattattcc ttcccctcct ctcaaaaatg tatgtaacac
    24301 gtatagatgc agaatttgaa ggtgatacct ggttccctga aattaactgg gaagaatggg
    24361 aactgatttt ctttgaacag aaaaaaagta aaactggcta tatgctaacc ttttctgaat
    24421 atgttcgtaa aaataggaat tggaaacctg aattacctga ggaatagaaa ttatttagca
    24481 tctatgccaa aaaactatct tttcctttgg tatactaatg atattaataa aacttttaga
    24541 tggatatagg aaatgaaaat atatagaatt tctgcgattt taatactcat agctattctc
    24601 tttggaggtt gcggtcaaac cgttcaatcc ggtaaagttc aagttagatt ctggcatggt
    24661 ttgggtggtc ccttaggaga tgtattaaat gatatgataa tggatttcaa tcacactcat
    24721 aaagatattg aaatcatcgc caatcctatc agcagctata atgctttatc tcaaaaactt
    24781 tccgctgctc ttcaagttgg taaacagcca gacctggcac aggtctttga atcctgggca
    24841 gcaaaatatc aggaagataa tgtgttggtc agtattgata gcttaattgc caatgaccct
    24901 gatatgactg aagaagattt aaatgatttc tatcccgtat ttatgaattc tatcacttat
    24961 aaagggaaaa aatggtcgtt ccccttcaat aaaagcgttc gtgcctactt ttataataaa
    25021 gatgctttct accgtgcagg attagatcct tactattttc ccgctacctg gcaagaattc
    25081 agggaatact gtaaaatact cacctcccct aaagatgata tttatggtgc taattttacc
    25141 gtaaatgaat ggcagtttgt taatctcatc catcaagctg gtggcactat aatggatgag
    25201 aataaccgtc ctgtgttgaa tagtcaatac ggtttagagg ctttaaatta tatacttggg
    25261 ttggttaatg ttgataagac cgcaaaatct accaaagagc ttgatggaca aaatgcattt
    25321 ctcgcaggaa aggtcgctat gtatgaaggt tccagtgtat ctataacttt tatgcgacaa
    25381 caacccataa attttgctgt tggttatgca ccattaccta cttatagaac taagaaaagc
    25441 gctattagcg gaactaatat tgtaatcttt aaaagtggta atcccaaaaa agaaaaggct
    25501 gcctgggaat tcataaaatg gttcacttcc acggaaagaa ctgcagaatg gagtgctcgc
    25561 acctgctata tgcctttacg caaaagtgct atgcaaactc agataatcca ggatttcttg
    25621 gctgaaaatc ctcaatttaa agatgtatat gcacaattag aagatgctgt ttttgaacct
    25681 caacatcccg cctggttctt aggaagagat gaacttaaaa aaagcctgga aaaagccact
    25741 gcaggaattc ttactccaca acaagcactg gatgaagtag cagctaagtt agaaaagctc
    25801 gtcaaagaag aagagaaaaa aggaaaattg taaaaatgtt aacgaccttt ctaaaatctt
    25861 tgactatcat cctgcaagaa tacagaataa gcttgacaat catttagtaa aattaaagta
    25921 agttctcaat atgccgaagt ggtgaaacag gtagacgcag tggactcaaa atccaccggg
    25981 cttttagccc atgccggttc gattccggcc ttcggcacca ttaataaaat ggagtgatta
    26041 acttctaaaa agttgagacc aatagcatta tttgtagaac gaaatagtgt aactgttttt
    26101 tttgggggtg tttcttcccc ctgaacttat aacaaagaaa aaaaataact cggggttatt
    26161 tattgctctg agttaacgga tattttttgg aggtatgatg tccaaatata ccatattaat
    26221 cctggcaaca atgatgattc tattgagcct gcccttcaca gtggatgcaa tttcccaggg
    26281 tgcattgcta tttctcactt ttgaacctgg tgctcgagct aatgcccttg gtcgtgctta
    26341 ttcagctgta gctgatgatg cttatgctgc ctggtggaat ccaggtgcaa ccgcatttaa
    26401 tcgtaaaaca caattagcgg gaactcatat accctggctt caaggagctg gtggtggttt
    26461 tgatgatatg ttctatgaat atttaggttt taaccactat ttccaagata tcggtaatgt
    26521 gaatgtgcaa tttatcctac ttgatgctgg aactcaggaa caaacggatg aacaaggaaa
    26581 tcaactggga acctttcata gctttgattt tgctggagga gcaggttata gttatgaagt
    26641 aataccagaa catttaggtg tgggaggaaa ctttaaactt atttatagct atcttggacc
    26701 tggtactggt aatactgaag atgaaggtaa aacttttact tttgcctttg atataggtgc
    26761 caaatatcga gacctgggtg taaaaggttt agaccttgct ctggcgatac aaaatatcgg
    26821 acctaatgta acttatgttg atgaatccca atcagacccc ttacctatga cttttcgcct
    26881 gggtgcatct tatcaagttc tggatacacc tatgaataaa ttgattgttt ctgctgaagc
    26941 cagtaaaata cttgctaatg atgacccttt atttaaacgt tttataaccg gttgggaaca
    27001 ctttgacgag acaatgtatg gatttggtgc agaatacacc tatttgaatc taatttctct
    27061 gagaggtggc tactttgctg atcaggcagg aagtattgtt ggtccaagct ttggcgctgg
    27121 gattcaatat acctttagta aaaaatataa gctaaccgct gattttggta tgataactgc
    27181 cggtgaactg acagattata acaaagtatt ttctttaggc tttgaattct gattaagaga
    27241 acactattcc cttcagcttc ctcaggtata gtcctgagga agcttaactt caaacaaatt
    27301 ctgagagctg gtataatatc tttatttctg ataagctcca ctttactata tgctgaaaaa
    27361 attaatctga gtaagtatta tcctactcat ccacctaaag ctcatcctta tatacaattg
    27421 cttaataaac agtcaggaaa gctattagat aagaataaag ataattataa taagttatta
    27481 gtaattctgg ttgattttca agaagaaata ccggatgacc caaataccac tggtaatgga
    27541 aagtttcagc ttgaacctga tcctaactat ctatacagta ttggaagtcc tcctcataat
    27601 cgtaaatatt ttcaggacaa cctggaagcc ttgcgatact attatcttgc agcaacagct
    27661 ggatctttca atctggaata tgatgtctat cctttaaatg gagcttatac cttaccccat
    27721 actatgggct attataatcc tccatcagca tccagtgaaa cttttgtttc caaaatggaa
    27781 gaatatttca gagatgcctt taccactgcg gataatcttt cgccggagat agatttttct
    27841 gcttatagtc attttatgat tatccacgca ggttcagatt ggcaacatga tatattttcg
    27901 gacacacctt cagatctacc ttctttcttt ataagcgttg gagaaggaaa agaagtcccc
    27961 gtagatgatg gttcattttt tatcagttat gcctgcaatg taccctccac tatatctcaa
    28021 gattttaccg aagaaattta tgagggtaca gttattcatt ctggttatgg tgctctcaat
    28081 tcagttatgg ctcatgaatt tggtcattct ttaggaatgg ttgatctcta taatgtctat
    28141 acttggcaac ctatggtagg tgtctttgat attatggata gtggaggttc tggagttcta
    28201 ttggacatct tagaagatgg gtcatatata atgctggaag gtggattacc cgttcttcca
    28261 ggagcctggt ctcgcacttt aatctgtgag gattatctta aatcaaaagg tttattatta
    28321 gatctggaac agataccact ttttgaccct attttacttt ctgctagtag ctggaaacag
    28381 tctggaaatt catatcatcc tcagattctg cgtattcctc tttcttccac agaatatatc
    28441 ctggttgaaa ataggaatgt agatcctgat ggagatggag ctactgcagt ttttgctaac
    28501 gaagataaaa gagttattct ctatcctaca gctattgaag acccggataa tagacctacc
    28561 tatgaatatg attatctttt gccctctttt cagaaagctg atggttctgc tatgggagga
    28621 ggatttctgg tctggcacat caataatgat gtgatttaca atcaaggagt ctgggataat
    28681 gaaggaaatt tcatttctaa ctatcaaaat aactcggtaa atacaagata ttctcgtaga
    28741 ggagtggaaa tcatagaagc agataatcta cctgatatcg gttatcccta ttcttggtac
    28801 tggactggca cacaatatga atatttttat gctcacaaac cgttacttga ttctgatggc
    28861 tattttattc actggactca agaaccctgg aaacctgtat taaatagcaa tacaaaacct
    28921 gctctaaaag atagtcaaaa tataggctca ctctactggc ttaataatat gggcaatcct
    28981 tccagagata tgtccataac cgtatgtgca ggtaattttg aaaccacaca aatctttgat
    29041 tattcagatt ccaggataac atgcgctccc ttaataaaat caagtttctg cgattttaat
    29101 atccccctta tattggaaga ttccatccat ctgctttcgt attcagataa cacctggttt
    29161 gacttaatgg gcagttttcc ctggaatagt catactatag atttccccat aactacttca
    29221 gatcaagatg gaaatggatt ccaggaattg gtactcgtgc ataataatac tcttgaatta
    29281 atacaattct cgcaagacga tttagaaagt gtctccttaa acttttcaga gcatataact
    29341 acagctccta tttcttttga agatactatt tttgttacca cccagaatac tttatcatct
    29401 atccataata atcaggtaat aaattctatc cccttacaaa atgttaaaag actcggttcc
    29461 tttgatgaca aactcatagc actgtgtcca gatctgttat atataattga tacgattaat
    29521 ctaaccgtaa gtgaagagat tgaattacca gaaccttttg gcaactatga acccgtaact
    29581 ttttgtgcct tatctgaaaa tacctcaatg ctgtttttaa tgtcaaataa aggtaatatc
    29641 tatagatatg aaaagggata tctgcaacag atatatatgg caagacctgg ggaacatccc
    29701 acgcaaatag ggctaacctg ttaccgcact ggtgaaactt caatttctcc tgccatattc
    29761 tggggttctg ggaataaaat ctatgctgta acctttgatg gttctttatt aagtggctat
    29821 ccttatgata tatctccttt cactattgaa cctgaagctc acctaatagc aatgcaattc
    29881 aattcatcac cacttttata tttccctcta tctgaaagag gatttctggc attttctcct
    29941 gataatggga ttttgtggca gtattcatta ttatcaaacg gagaaacagg aaattctcat
    30001 ttgatgataa tccctgatca tcaactttct tcccatttat actggtatta cacagatgga
    30061 agcggaaaaa tatatattca agggaaaaat atccttactt ctgaaacaca actttgctgg
    30121 aatggcttcc gcaatggagg aaatggcact ctttatggag aattcagtat atccgttcct
    30181 gctcaggctt ttaatgccta tattttccct aatccagtta aaggagataa ttttagagta
    30241 agaattgaaa actttaatca aactgtcaaa ctccatcttt ataatatcaa tgcagattta
    30301 attaaaagtg agactgttcc tgcaaatgga aatctccagc gtgatataat aattgacagt
    30361 cgcaaacttt cttctggagt atatcttatg aaggtacagt gtggaaataa acataagacc
    30421 atcaagtttg cagtacaaaa gtaattgaga ggagaagaag atgaagaagt ttttattaat
    30481 tataatttgc ctggggttat tgtctactct tttaggacaa ggtatagatg atttaatctt
    30541 ccaggaaaat aagaaggact ctgagcagga gataataaag gtcaattttg aaaagaaaga
    30601 tgcaaggttg gcgatgcttt attctgctgt tctacctgga gcaggtcaat tttatgctaa
    30661 accttctgct gtgattacct atatcttccc tatcctggaa atcgctatga ttggcggaat
    30721 gatttattat gataagcaag gcgatgaaaa aactaaagat tatgaaaaat atgctaatgg
    30781 cgagatagtt actcatacct tcaattataa tgtaggtgga gttgattatt cctacactta
    30841 taccggaaca cgctatagac gcgactatca aactcaggtg caaaatgttt tgaagaatat
    30901 aaatgctttt gatatctatg acgagacctt ctttcgttta gaccaatccg atactcagca
    30961 tttttatgag gatataggta aatataataa gtatatcttt ggttgggcag attggtatca
    31021 tcgctttgcc actgatccca cttcaggaga cggaaccttt attatggatg accctgctta
    31081 tgaaggactc tgggttttta ccggaagcac cgaccctcaa ttaatccaga atagacgctg
    31141 gtatcagaat tataccgtgg aagactttat gaatggagac ctatctcatc ctatatctcc
    31201 aaagagccct gaagcttcac cctggcgtca ggaatatatt aatatgagaa aagatgctaa
    31261 taaacaatat gactattccc attactttac acttggttta gctgcaaatc atatcatttc
    31321 tgccattgat gcctatatta tgactaatca agttaatcgc atagctataa ccaaaaatga
    31381 taaagttaaa atgttttatt atactgatgt ctccaataac agtctaaccc ctactttagc
    31441 cttaagagta aacttttaaa tctatgatga aacaccttgt cttccttctt ctgattcttt
    31501 caataatgcc cttagccgca ttacctaaaa taatatattc tgctacttct gctatcttac
    31561 cagggacagg agaactggca ctaggaaaaa caactcaggg tgctattatg ctcagtgcag
    31621 atattctggt ctggagttcc ttttttgctc tggaaaaaga aaaagataat ttgactgata
    31681 cctataaaca atatgcaaaa gtctatgcgg gagctccaga aaatatgcct gcaagttatt
    31741 atcaaaatct acaaaattat ataagcagtg acgattttaa ccgctatcag gaactttttg
    31801 cccggaacta ttatttaatt tattcttata atccagaagg ctatgaagct tatattttag
    31861 ccaataccta tagtgatgaa gaaggttgga gctggcaaaa ccagacttat caaaatcatt
    31921 ataaaaagtt gcgcagaaga actcaaaaag ctaaaatcta tcaaaatcta agtgttggag
    31981 ccttgctcct gaatcgggtt gtcagtgtca tagatgtgtc tcttacaaat aaaaaagcaa
    32041 aagagccatt atcaatctat tttactccta cctccaataa cggtttaatg ttcaatgtcc
    32101 agctggagta ttaatgctta ataaattcaa agtaactttc tttttaatcc ttttgctatt
    32161 gttgctgact gtttctcttt ctgcagaaag tcggggtgtg aaagttttaa aatctctggt
    32221 agtgcctggt ttatcacaaa tacaggatgg acataattat ggctatgcga tgctttcagc
    32281 ggaagcagga atcatatcca ctctttttta ttttaatgag gaagaaaaac taaaagcaca
    32341 aaaatactat gaatatgcca tcaaatttgc tcatatagaa ccgggaaaat atgacgacca
    32401 atattataga aatttgagcc gttataatag tagtggtttt gaagctggtg gatataatgc
    32461 taaaattaga gaggaagcta tgcaattata tcctaatgac cctgttcagc aacaagctta
    32521 tattgatgaa cattgctatc cagaagagta tggctggaat tgggatagct tagaacaccg
    32581 tcgtcattac agtcaactta gaattcaagc aagagatctt caagattatg gaaaattaac
    32641 tatgggagtg ctcatagtaa atcacctaat cagtggaata gatgctcttc gttataccgg
    32701 taaagaaatt aatcctcaag tatatctgga tatcaaagat aaccaacccc tcttaatg
//
LOCUS       DAORZF010000013        31609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_1194_length_31609_cov_377.619953, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000013 DAORZF010000000
VERSION     DAORZF010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 31609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31609
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1194_length_31609_cov_377.619953"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="PK015_02785"
     CDS             complement(<1..854)
                     /locus_tag="PK015_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOL33432.1"
                     /translation="MTVTKYVYLFGNGKAEGNAAMKDLLGGKGANLAEMDRIGIPVPP
                     GFTITTEACIAYTKYGTEKIKELLEDDVKSAMKHIEEIMQMQFGSNENPLLVSVRSGA
                     RASMPGMMDTILNLGLNDNSVQGLIKKTQNERFAWDSYRRFVQMYGDVVMGLKPENKE
                     DLDPFEVIMDSLKKEKGVTSDLELTASDLKELVYLFKKAIKERTGKDFPDDPWEQLWG
                     AIFAVFDSWNNPRAIFYRKLHNIPDDWGTAVNVQAMVFGNMGNNSATGVAFTRDAATG
                     EHIFNGEYL"
     gene            complement(1060..>1541)
                     /locus_tag="PK015_02790"
     CDS             complement(1060..>1541)
                     /locus_tag="PK015_02790"
                     /inference="COORDINATES: protein motif:HMM:NF017927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HOL33433.1"
                     /translation="EDFKIEEMFNGFKYFGRALGTYLLRALYILLWYILLIVPGIIAA
                     ISYSMTFFILAENPNIKAADALRLSKQMMYGHKTQYFMLMLSFIGWFLLSILTFGIGL
                     LFLYSYITMASTIFYQQIKGEALYNEIIIENVEQSTKSPTEDSDKSTNQDSPYQDLY"
     assembly_gap    1542..1641
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1642..2942)
                     /locus_tag="PK015_02795"
     CDS             complement(<1642..2942)
                     /locus_tag="PK015_02795"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOL33434.1"
                     /translation="MLYQEFLDHIFQKYSANVKLELTRMQNLLEAMGNPQNSLRGLHI
                     AGTNGKGSVCATLEALALAHNLTVGLNTSPHLINYTERFRINGKDLSFETILDIYLRY
                     ENLFEKYEASFFEITTAIAFQAFKEAKLNTVIIEVGLGGRLDATNLFTPDITAITTIG
                     LDHIKTLGGTLEQIATEKAGIIKENVPLVLGNIAESPLNIIIEKAEKLNAPVYLPQKD
                     YFYQLNQIDLNGVQFDYSFGPYTFQNLNTNLLGEHQAANVAQALTAFILYSEKRGFSI
                     SPPKIYSALQNIHWQGRMQVLSSSPIIIADGAHNVHGVHALINSLHNLFPGRKFRFLV
                     SILADKNYKEMLALICANAEKIYIAQNESDRAATVEMQSAEISKYEIPYETASTVPEA
                     FIKAINELSKNDILICCGSLYTVGEILNYYQQSDNFIKQAG"
     gene            complement(3056..3733)
                     /locus_tag="PK015_02800"
     CDS             complement(3056..3733)
                     /locus_tag="PK015_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33435.1"
                     /translation="MLKNQKGNVVFWIVSAILAIALVFILALPGSFNLDPQKNTDDCT
                     TNMKNIWVATNDYMLDTNKDFQGNLNILRTTKKPGSKYFYLNEEKYCPESQGNKVDYI
                     VFGKHVTEDFEGATRHYNGIIIICPNLARFPKHILEKSFYDNVSITKLQNVMANDIDK
                     INTYTKSNGKLKYESLMRYMNYWKNTKHTEFNACVNDPEYIALRSELTGESSGTVPGA
                     QTVSETE"
     gene            complement(3791..4204)
                     /locus_tag="PK015_02805"
     CDS             complement(3791..4204)
                     /locus_tag="PK015_02805"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOL33436.1"
                     /translation="MTLGVAIYYKIDPKIFYHLIYTESKFYNFALSPSKAKGLGQIKE
                     STAFYIHQKHKPGKLYFPMYNLKLSAKYILYLQERFQGNWTLVLAAYNWGEYNVAQRI
                     KGMSIDPHADYRYKFKDIQETYDYINKIIPPAKKA"
     gene            complement(4321..4806)
                     /locus_tag="PK015_02810"
     CDS             complement(4321..4806)
                     /locus_tag="PK015_02810"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOL33437.1"
                     /translation="MAVYSINEIIEMAVQIERNGYAFYNEATKRKDLDDKSHEFISWL
                     RDQELEHEKKFLSLRDDTELKSLELSQDWELVALYLKTIVEGRIFNSEDSAIRRAIEA
                     KNITEIIDYAISFEKDTLLYFQAISNNIAYPKAIDILHQIINEEISHIIKLADYKKNI
                     S"
     gene            complement(4874..5503)
                     /locus_tag="PK015_02815"
     CDS             complement(4874..5503)
                     /locus_tag="PK015_02815"
                     /inference="COORDINATES: protein motif:HMM:NF024805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOL33438.1"
                     /translation="MEDLGAYLKKLREEKNISYDQIYEDLRLREEQVRLMEENRFAEI
                     GYIGFARAMVFNYARYLEADLDEVMQKFNLLMPENIKKVEPETQEAGKKILLSPNLFW
                     IIGIILIVVILGAILWHAHNAGWLQMPDIFKTSSADTTTVEKLTQPEEKDITQKDPIR
                     ERQKALMDSITKAQTTQKVTVQKNQPSNIDTMDIVGEIIGPSIMDISDQ"
     gene            complement(5491..6267)
                     /gene="dapF"
                     /locus_tag="PK015_02820"
     CDS             complement(5491..6267)
                     /gene="dapF"
                     /locus_tag="PK015_02820"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOL33439.1"
                     /translation="MLIPFSKMEAQGNDFVILELIPALNTNLPLNILAADICKNHTGV
                     GADGLVILLASEVADGKMIIYNKDGSRAEMCGSALRCCASLLSGYTGKKELKIETDSG
                     VKRANIKAEGIQVSLGQPALIKDNIWVEGIEGTLVDIGNLHFVSYHDVLNGQEQRLGP
                     LLEKHPSFPGPVNVEFVHPYSAKEIEMKVWEKGVGFTQACGTGAVASVFTGINRGILA
                     DEVKVNMPGGEVLIRKLENGEFLLEGPVTNVFNGVYIWKI"
     gene            complement(6261..7001)
                     /gene="dapB"
                     /locus_tag="PK015_02825"
     CDS             complement(6261..7001)
                     /gene="dapB"
                     /locus_tag="PK015_02825"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOL33440.1"
                     /translation="MLNITLIGYGKMGKMIASLAEQHGCKVKSIIDPHQEGCEKEIRE
                     DLLGEVCIDFTQPQSVLDNIKKVAAAGKHMVVGTTGWNKYEDEVRKIVEANNIGLVFG
                     ANFSIGMNIFNRIVRYAASLCDKFPIYDVCGWEMHHNQKADSPSGTAIQLAETIISQS
                     STKETAIYDRLNRPINKSELHFASLRGGSEPGWHNIIFDSENDTIELSHHVRSRACFA
                     TGALKAAQWISVHKGMYSFNQMMEDLLC"
     gene            complement(7011..8135)
                     /locus_tag="PK015_02830"
     CDS             complement(7011..8135)
                     /locus_tag="PK015_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOL33441.1"
                     /translation="MQDEVVNYFLELISIDSESGDERAIIDRLKADLKALGAKVEEDD
                     AYILTGGNAGNLYAQIPGNLNKKPILFCAHSDTVKPGKGIKPIVKDGIIYSDGTTILG
                     SDDKSGIAELITAIKEIIESGEKHPPIEVLITVSEEVGLLGAKHFDKSRLESAWGYAL
                     DTHQVEELVIGAPAQNSIQITFLGKESHAGVEPEKGINAIRVAAEAITAMPLGRIDHE
                     TTSNIGLINGGVATNIVPNKVEVKGEARSHNHFKLEQLTSDIRHCVERAVQHFQYDFG
                     GASFDWKVNLEYEAFHIQESEPVVQIAIDAMHRIGLTPKITIGGGGSDANIINASGLP
                     MIICGTGMNKVHTLNEEIEIEQLQKGKEFLKSLIRRYAEL"
     gene            8470..8964
                     /locus_tag="PK015_02835"
     CDS             8470..8964
                     /locus_tag="PK015_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33442.1"
                     /translation="MNKSILYTCELKHHRQVAAPLCSVCPRVAKCRSFRNWFDIYRDE
                     YLDFVINICNKFPEKYTLEVTLMAEKQTFVQIVDMSTGMIEKIVNLKEIEALTPEEKL
                     ELARNKNLFIVTHRIVPIVRVEMKKTVIEEPMQFGGNNEKKRQEEAEPIVEPVSPAKT
                     KKKK"
     gene            9098..9463
                     /locus_tag="PK015_02840"
     CDS             9098..9463
                     /locus_tag="PK015_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33443.1"
                     /translation="MFKLADEHLEVAQKIAQKHRRKKHCDHCYDRGWIGINEQNLLVL
                     CPHCVDREAALEEWKTYVSEHEDLKEHFSELFEEKPVEEEETESTIPVAHTKKPAQPQ
                     VKKTFVPGQKKFGRTKKIG"
     gene            9519..12644
                     /gene="ileS"
                     /locus_tag="PK015_02845"
     CDS             9519..12644
                     /gene="ileS"
                     /locus_tag="PK015_02845"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOL33444.1"
                     /translation="MYKNIDLKATPQDLEGSIRQYWKQHNLLQKSVLFREGAPNFIFY
                     EGPPTANGTPGIHHVMARTLKDVVCRYKTMTGFQVKRKAGWDTHGLPVEIEVEKQLGL
                     ESKKAIEDYGIEKFCQACKESVWNYLDLWTKMTELMGYWIDLEHPYVTMYNEYIESVW
                     YILNDFFKRGLLYKAHKIVPYCPSCGTPLSSHEVAQGYKEVEDPSIYIKFKAREEENT
                     WYLAWTTTPWTLISNVALAVHPDFTYVKVFHKGEYYILAKERLDVLDSEYEIISEFSG
                     RELEYKQYEPLFNFVPVDKPAWYIGLADYVSMEEGTGIVHTAPAFGQDDYQLGKKYNL
                     PLVQPVDAEGKFTSPVEPWAGIFVKAADKDIIRFLKEKGNLYKREQVKHSYPHCWRCQ
                     SPLIYYARDTWYIRTSDFKEQLLENNRQIRWYPPFVGEKRFGEWLENNVDWALSRDRF
                     WGTPLNIWICNDCGARTSIGSIQELRDKGTLKNGLPIPENLELHRPYIDEVVLKCEHC
                     GGTMIRTPEVIDCWFDSGSMPFAQWHYPFENADNFDKELFPADFISEGIDQTRGWFYS
                     LLAISTLFKGVSSYKSCLVNEMILDKNGQKMSKSKGNAVDPIELMQEYGADAIRWYLL
                     EVSPPWVPTRFDVDGVREIVGKFIGTLKNTYSFFATYANIDGYDASSYSLDWQRIAEL
                     DRWIISRLHSLINKVREWMEEYELTRAVRAIQDFVIDELSNWYVRRSRRRFWAAGMTS
                     DKIDAYNTLYQVLVTVAQLVAPFAPYLSEELFINLTGKESVHLTDYPLSHQEVIDLEL
                     ENKMQVVIDLVSLGRAARNACQIKIRQPLEKMYLPAKYKDIVESMRELILEEVNIHNL
                     VYVSEEDDFVQYEVKPQFKIMGPKYGKQMKAITEALSKLKGQEVLSAFNSSGVYHLTD
                     LGIDLVPEDVVVQIIPREGFVFESMNDKFVALDTTLTPDLLQEGYARELVNKIQFTRK
                     EQDFDILDRIVVEWYGDDDIQAAIDKYNDYIKKETLSDELRRVNSSQNMQVYDINGKE
                     VYLKIYKVENK"
     gene            12641..14677
                     /locus_tag="PK015_02850"
     CDS             12641..14677
                     /locus_tag="PK015_02850"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOL33445.1"
                     /translation="MNEGQNSGMNKLFCSNCGKKIPINAKFCSYCGQKIEGWEEHPSQ
                     EDKIKPQKKVIKREPESEDMSFVAYQTLEPGTSFYNYKVIKLQGKDTDGVHYLVEKEG
                     KQYVLKLFFPYKYSNLENIITLQNWLNRVQKLENPYIVHVEEINYNNDPVYIVTEIVE
                     GISLSKIKTYNPELLTESLVREIAKQLVSAAMDIHQLGLSIKNLTLNDLILNPETNTI
                     TILTSGISYETTDERDEVFHLGVLLAQMLSANQLAETVYNDEILSEHKFTYIPGITLS
                     MNKVLGECLHRNVLQRYNTLRDVLKGLNALIDIEKDEIYELSGNKLQPEDISPMQVPE
                     PKTSKDRIIITAIVIGVAAIVIFFAINWSKITKDNGTSSTWISSIGEEDTINNEYQNK
                     TIFNHRDTLKNQNYNTIDSRVDPRKAILSQNQSSYSGSSGSTVKIDTSPDRDLFVYVP
                     DGTFGFTRLKENPNHNVVQNGFYMGKYEVTQKEWNQYMKPANVSFVGDNLPVDNVSWM
                     NIVRYCNARSEAEGLTPAYTILGNNASSVTCNFKANGYRLPTEAEWEMAAKGGQLYTY
                     SGSDDADEVAWYRENSGRRYRTVGYQKKPNAYGIYDMTGNVAEWCWDWYSEDYPSSLT
                     EFVNPRGPASGTLKVIRGGSVNNGLGTNLSVVYRTKGDPAKGYPFVGFRLVRTH"
     gene            complement(14680..16980)
                     /locus_tag="PK015_02855"
     CDS             complement(14680..16980)
                     /locus_tag="PK015_02855"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOL33446.1"
                     /translation="MQKRYLPFILIFFTVLTIKIQALPSCYYTYDQISQLLSTYESQY
                     PDIAKKVLIGYSQEDHIPIYAMRITDNVNQDEEEPALLFVGQVHAEEVLGVQITMSNI
                     AEILTNRYQLPYMQWINQLDLWFVPTLNPEGHNVVTSNLDSSYRKNKRDNNMNGIFDF
                     SPQVGYDVDGVDINRNFSFNWVHGDTLFQPGGLEVWDYYRGPAPMSESENQAIKELAD
                     RYKFIYSICWHSSRTGNLSEKCYYSFNWKNVRPSPDLAFSASIASGIASHIIKETGTG
                     TYDYFPNLSRQGAFHDWMYQQYGTFQVLIECGTSNIQPDSTLMVNTVQRCSNGVRWLI
                     NRALPSSEAVSSNSMLTGKIRDAVTNEPLEAEIIIQQHNAPWFRPRTSNPTTGRFYRP
                     LASGVYTIQVRKKGYWDTIIPSQMVQNSNWTQIQVYLQPREPAILNGNINANGENINA
                     RIIIGDVFPDTLMVNGNFIFHGYEGEYPVEIYAEGYFPWNGTVTLSPGENYLTIELST
                     VNILFSEDWENGTDRWEINGPWVRENTLSVSGYAITDSWGGNGFYAMNCDVWIKTAQP
                     IYIPIEASTMLIFDSHLYTEYQFDPARVEISTDNLNWECIWEKSGELSYFQKEMVSLD
                     SFQGQNIYLRFHLTDNSTDIDLTDPGWTIDNILIINGTATPNCDPSLPAIPRFVLYPP
                     YPNPFNPETTIRYSLAEPTNITLNIYNTKGQMVRQLEKCTKNAGAYQVIWNGKDNSGK
                     DVSSGIYFCVLSGGKDKQFQKMVLCK"
     gene            complement(17130..18389)
                     /locus_tag="PK015_02860"
     CDS             complement(17130..18389)
                     /locus_tag="PK015_02860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOL33447.1"
                     /translation="MHILYISYFYPPLGGPASLRNVKVVKYLAQEGISCDVITVSDIE
                     YIQRDFSLLSECSEQRLIRTDSLDPMALFRKTGISKGNQATGLYMNTPENIKLWIRRL
                     YPLDEKIGWNPFLIKAGRKAISETHYDLIYISLGPFSSGVGAYKLSKESGIPLVLDMR
                     DYWNLLSDYNLQATTLHRKYAWNWEKRFYSYASLIVTATQGIERDIIKAFGPEFAEKT
                     LTVYNGWDENDFIDLPAVPKPSEYTFSYFGSIYARRNMNAFYKALHRLLEEGKIPQNI
                     KVRLFGNYFKETREEITQSGITNLIEIIPPLEHREALANMMASDVLLLVINSSSPYGT
                     LTSKVGEYLRTQKPILAMVPYYGEAAELLRECGHNYICAMESADTIYHSLKRLFSETE
                     HNYQIPWEMEKSKQVHNLANRLKEMLG"
     gene            complement(18391..19524)
                     /locus_tag="PK015_02865"
     CDS             complement(18391..19524)
                     /locus_tag="PK015_02865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOL33448.1"
                     /translation="MKICILSNAHPTNEVRLYYKLGRSLAKIGEVHIICASGVCNSLV
                     NPYQDVTEADSKWGILFQLYKKAKKIHPDVVICVEPLTLLVGLALKKKIKCKVVFDIH
                     EFYPDAFSEKFPLPFKWIMKSIYLGLERSLQCKVDGTIAVNEQILQQLGRAPKKGEVA
                     VIPNYPVKNVWDDSCEVPMELSNICTMNFDLIYIGGLTPDRGAYKILKSINILKDEFP
                     QLKALIVGKFTSPEVEKKFDAMLTEYNLTAFIYYNNWVPPEKIGLLLKRSKIGLWLFN
                     AQNKRLSKALPLKVLEYFAAGLPVVSTRTPLMSDLIEKNQLGYCCEYHSASIAKAISR
                     ILKLDEDKYQEMSKRCIDLIENKYNWESVEPELLDLIQNLASK"
     gene            19955..20599
                     /locus_tag="PK015_02870"
     CDS             19955..20599
                     /locus_tag="PK015_02870"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOL33449.1"
                     /translation="MSSKKINRPTLSFSNDKKRERKVSDTQKESKDNEEKKKNKEKYP
                     DNSVLQWWSSIYFDYLKQEANNPDNPHPLMASVEVEVVKEADLEQILALYKQAGWWNE
                     PIDEKSYERVRRIISDTFCFVIARYQDRIVGMGRSISDGVSDAYIQDVVVDKDFRRQG
                     IGEAIIHRIVQFLLEHNIRWIALISEPGSEEFYRQIGFRDMMGYTPLFLPIENI"
     gene            20608..21489
                     /locus_tag="PK015_02875"
     CDS             20608..21489
                     /locus_tag="PK015_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HOL33450.1"
                     /translation="MLELTPLTPAHIPLLQKYLRAYPRQSCDYAICNLMTWGKIYGNS
                     FTIWKEHLVIVNPKYDYVMYPVGPGLSAKELRELVDIYREGHPLTQLVLIPEDWQIKT
                     PDITQYFQLREDRDWADYVYLIENLVKLSGKKLAKKKNLISQFIRTYPGYQVIPITSD
                     KKDVIIRFSEKWRRERNMEGIYLNAEFQAIKNTMEMWDEIPVEGIIICYKNRISAFSI
                     FSEQTVDMVTEHFEKFDPEMKGSAQVINWETAKYLQDRYIWLNREQDMGLKGLRQAKS
                     SYMPAFLVKFIFGKPLD"
     gene            complement(21497..22291)
                     /locus_tag="PK015_02880"
     CDS             complement(21497..22291)
                     /locus_tag="PK015_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOL33451.1"
                     /translation="MYNFIYHAILAALLSSFSLSLFSPLVTLRQVSYMGEALSHIAFA
                     GIALALLLELNLQITTLIFVVIVALAISWLSQKHKLQEANTITIFLSVSMALGIILIS
                     LKKGYSFDLESYLFGNVLLVSPSEIYQLVILALLDIFFISFFYKELFYLSYNAEMAKF
                     YRLPVGLVNTIFMILLAANIVITLRAAGIILVSAQLILPAVTSFNLVKRLDYAILCSA
                     IIAILAASGGFALSWWLDIPTGANIVLLEFVLYLISLMFRRRQKIA"
     gene            complement(22291..23019)
                     /locus_tag="PK015_02885"
     CDS             complement(22291..23019)
                     /locus_tag="PK015_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HOL33452.1"
                     /translation="MIEIRSLSYKINGNIILEDINLTLAEGEFAALIGPNGAGKSTLI
                     KLILGFLPLQTGSIYIDGIPHWEWLKEHPIGYLPQHEEFNRHFPATALDIVLMGLVAE
                     LPPGSRFKSIHRKRAMQALSLTGIAHLANHQIGSLSGGELQRVFLARALISDSKYLIL
                     DEPEASLDQPGVQSLFILLKDLNKAGKTILTISHDLNILSEYCTFLICLNRKLHCHTS
                     AELVNAEIIHKTFGDTMRLIEKDY"
     gene            complement(23039..23896)
                     /locus_tag="PK015_02890"
     CDS             complement(23039..23896)
                     /locus_tag="PK015_02890"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HOL33453.1"
                     /translation="MKLKVLISILIISSLFALNSCKKAVDSPSNLIVVSIYPYELLLK
                     QMVGPEIEVRTLIPPNFSPHTYNPSPAELKSLYDADLVFSNGFGLETNIQTALNALGE
                     KHLSAEQLLQLPQKEENPNPHIWLSPRLMKKLVSELEEPLQRTFPSYKDSISFHCREL
                     ISQFSSLDSLIIQERAQFSHTPIITYHDSFHYFIQDYKLDYLGSIQSSPGQEPTPKEL
                     VYLGDLIKANQVKAIFVEPQMDRKSANVLAKEFHLQVIELDPLGQTLNAQSLPEVILI
                     NWNRMKQAW"
     gene            complement(23893..25371)
                     /locus_tag="PK015_02895"
     CDS             complement(23893..25371)
                     /locus_tag="PK015_02895"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HOL33454.1"
                     /translation="MKIDLLHKPTEYMETVIILQEENGDIEKLEYLPQYLQNAIKIVV
                     KEDDFQFKYASLKSFPFIRSRKRTNVILCGIGNPKELDNDRFRHLIAISIREALKLEA
                     KEAYIFTGYKNPLSELHFGHILAEAALLTGYRFDKYLSETKPPSLEAIHLAMDLKNPR
                     MFNRGVLEGRIFAEATNLARDLVNEPANVIYPESLAETAKKVALKYGFSIEVFGLDKI
                     KRLKMEAFLAVGKGSKKEPRLILMSYHGNPDPKAKTIGLIGKGLTYDSGGYCLKNCQG
                     MVNMKNDMSGAAAVIGTMAAITNLKLKVNVIGIVAACENMISGSAYHAGDIIRSMAGK
                     TIEVVNTDAEGRLTLIDAIHYAISREHIDEVIDIATLTGAAAATFGNQISAVLANNEA
                     LLNKLQSASEFTGEKIWQLPLYEPYKELIKSEVADLKNTGGPLAGTITSALFLQEFVA
                     GKPWIHLDIAGTALRDKEQGIYSYGATGVGVRLLTQLLRELE"
     gene            complement(25397..28399)
                     /locus_tag="PK015_02900"
     CDS             complement(25397..28399)
                     /locus_tag="PK015_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33455.1"
                     /translation="MKLKHYLTLALLILVTFVQAYYFGQNKVNAKIEEWSTIQTMHFD
                     IYFPQGEDEFGKLAALMAEESYYYLKEQLKFPITSRIPIIFYRSKAAFQNTNIIYPLL
                     TEEVSGFTESLHNRVVIPFDGSYANLEELLIHELTHAYLNALERAKEESLASLYSSYI
                     PFWFSEGLPEYFSTLGKKDYNNMFILDMVINDKLPYIENTSGYYAYRLGESFLTFIAE
                     TYGPDKLMEYFFALRSTSGIDYATKKIFGLDFKELESRWRFQLKREFLPLIETHDLPS
                     LSLEQRTNSKRDGSYFNLMPRFSPQGDKYVYFSTAGARYSIWMGGLHGLSAPERIIKG
                     EVSGKMEEFYYFRSSLSWFPDNTRIAFSAKTSDGDCIYIVNTSKKKIVQTIKLKDFDA
                     IYELDVAPDGKSIVFAGQKNMQCDLYLYNLETKELQQLTDDYYNDLQPRFAPDGNSIV
                     FASERSQKEPEFPNRLFSHLNQNIFHLNLNNGICTQLTLETKDCGYPSLDASGTKLYF
                     VSSIDGVSNLFMLNLTSAQKAKVTNVLAGVYSADISPNGKQIVLSNYFQDAWNIYFTE
                     IQTENLSFIDYYPPQFVKLKNDLLETVDLSRLNYYGKRPQRPYMQKDPLIESAHSNEE
                     MGSSDSLRMYRNLSYDDKPITIETIPEIKPYKTKFSLDTLWGGLAYSSYAGTVGYLEL
                     SLSDIMGNHGIGISLSAAGKLKDSNLLISYLYLKQRMDYGIGLFNLNDLINIQMSIPG
                     PQNDLYIRYKQRQTGLYLLLRYPFNRFTRIELENMFYQQEYYLSYWDWNIGDWTEEEN
                     LGSDKVFSPAVNLVHDNALYGPTGPLAGRRLYYSVGTSLRDNNLDYLTNYLDWRNYIL
                     FSKRYSLATRLITGISVGDYPQRFALGGYYGIRAYDGNLSGEKKAVFCTELRFPFFEN
                     INIAFPLPLNIPNVRGSLFAEIGTVFDDFNKFQATHEGKLKDLYLGYGFGPRINLGIF
                     ILSLDITWLTDLSHISKPTYYLSLSEDF"
     gene            complement(28410..29312)
                     /locus_tag="PK015_02905"
     CDS             complement(28410..29312)
                     /locus_tag="PK015_02905"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOL33456.1"
                     /translation="MKRFYPNFHKTQVKVGLFTIIILAILIFGYLWLSSKITNRSQQQ
                     LKVCFDDVMGLDLGDPVLYRGMEVGRVKYIELQPDKILVTANINRDIKLQEGTKFQIT
                     DTSIMGGTALNIIPGNGEHNLDLRKIQKGDKPLGIMNAFDEVSIAIAKVNEALNELQG
                     GKIFTDKITTLLDDADSAVLSAEIMINETRTNLNNTLTKIEQLSKKLQYLLDNNAAHL
                     DSTLTLAPEAIANINNTLDSLQVLSTQLHNTLNDLNEGKGSAGKFLKDETLYKRLLET
                     TTSLDSLFQDIRTNPKKYINIKIF"
     gene            complement(29316..30569)
                     /locus_tag="PK015_02910"
     CDS             complement(29316..30569)
                     /locus_tag="PK015_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HOL33457.1"
                     /translation="MPVKKIIVLLIILLPALIGAEILIPMDFTQSNHLKAYGVAFEAL
                     KEQYVVKWFLNYRGGSFLMPESESLAALCNIRGVRFELVSSAQVASIMQEIQESNMEV
                     VVLEKEPKIAVYIPPNALPWDDAVTLALDYAEIDYDRVWDDEVLAGKLSDYDWLHLHH
                     EDFTGQYGKFYGAYRNTAWYQNDVRVNEAMAAKHGYAKVWQLKHAVAEKIREFVMNGG
                     FLFAMCAATDTLDIALAAHNVDIVDALIDGDGIDPHYQEKLDYSRCFAFDNFKLKTNP
                     FEYEFSDIDASDYSRLRGPEADYFQLFDFSAKYDPVPTMLTQCHTNIIPGFLGQTTSF
                     FKDKVKKSVIILAEVPGQNEVKYIHGNFGKGTFTFYGGHDPEDYEHKIGDPPTILDLY
                     KNSPGYRLILNNILFPAAEKKQLKT"
BASE COUNT        10032 a         5626 c         5810 g        10041 t
ORIGIN      
        1 agatattctc cgttgaaaat atgctcaccg gtagcagcat cacgagtgaa agccactcca
       61 gttgcactgt tatttcccat attaccaaag accattgctt gaacattaac agcagtgccc
      121 caatcatctg gaatattatg caatttacga tagaaaatag cacgaggatt attccagcta
      181 tcaaaaacgg caaaaatagc tccccatagt tgttcccagg ggtcatccgg gaaatctttt
      241 cccgtgcgct cttttatggc tttcttgaaa agatagacca gttctttaag gtcacttgcg
      301 gttagctcta aatcactggt gactcccttt tcctttttta aggaatccat tatgacttca
      361 aaggggtcca ggtcctcttt attttcgggc tttaaaccca tcaccacatc accatacatc
      421 tggacaaaac ggcgatagct atcccaggca aaacgttcat tttgtgtctt tttgattaat
      481 ccctggacgg aattatcatt caaaccaaga ttcaagatag tatccatcat tccaggcatt
      541 gaggctcttg caccacttct gaccgaaacc agaagcggat tttcattaga accaaattgc
      601 atctgcataa tttcttctat atgtttcatc gctgatttta catcatcttc cagaagctct
      661 tttatctttt ctgttccata tttcgtgtaa gcaatacaag cttcagtagt gatagtaaaa
      721 ccaggaggaa ctggaatgcc aattcggtcc atttctgcca gattagcacc ttttccacca
      781 agcaagtctt tcatcgctgc attaccttcg gctttgccat taccgaatag gtaaacatat
      841 ttagttacag tcatttatcc tccaaaatac attattttct tattacttta actatctttt
      901 agaatagctt tttttgtcaa ttcataatcc tctatagaat aggaataatt aaagagaata
      961 gatagataaa aactctcttt ttagcagtta aataatgaaa ctgctaatat aaacttaaaa
     1021 cctttagagg tctaagatat aaacatagca ttaaataatt taatataaat cctgataagg
     1081 ggaatcctga tttgtagatt tatccgagtc ttcggtaggt gatttagtag attgttctac
     1141 attttcaata ataatttcat tatataatgc ttcacctttt atctgttgat aaaagatggt
     1201 agacgccata gttatataag aatataaaaa caataagcca atcccgaagg ttaaaatgga
     1261 taataaaaac catccgataa aggataacat taacataaaa tattgagttt tatgtccata
     1321 catcatttgt ttacttaacc gaagagcatc tgcagcttta atatttggat tttccgctaa
     1381 aataaaaaag gtcatagaat aacttatcgc tgctataata ccaggaacaa ttaataatat
     1441 ataccagagt aatatataga gggccctaag aagataggtt cctaaagctc tcccaaaata
     1501 tttaaaaccg ttaaacatct cttcaatctt gaaatcttct tnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn ntatccagct tgtttgataa aattatctga ctgctgataa
     1681 taatttagaa tttcacctac ggtataaagt gaaccacagc aaatcagaat atcattcttg
     1741 ctcaattcat ttatggcttt tataaaagct tctgggacag ttgatgcagt ttcataaggg
     1801 atttcatatt tactaatttc agcactttgc atttccacgg tggctgctct atcggattca
     1861 ttttgagcga tatagatttt ttcggcatta gcacaaatca aagccagcat ttctttgtag
     1921 tttttatccg ctaagataga aacaaggaaa cgaaatttac gaccaggaaa tagattatgc
     1981 aaagaattaa tcaaagcatg aacaccatgc acattatgag caccatcagc aataatgata
     2041 ggagaagatg acaggacctg cattctacct tgccagtgga tattttgaag agcagaatat
     2101 atcttgggag gtgaaataga gaaacctcgt ttttccgaat agagaataaa agcggtaagt
     2161 gcctgagcta cattagctgc ttgatgttca cccaggagat tggtattaag attctggaaa
     2221 gtatagggtc caaagctata atcaaactgg acgccattaa ggtctatttg atttagctga
     2281 tagaaataat ctttttgagg tagatagacc ggagcattta atttttctgc tttttctatg
     2341 ataatattca atggactttc agcaatatta cctaaaacca gcggcacatt ctcctttatg
     2401 ataccggctt tttctgtggc aatctgttcc agagttccac ctaaggtttt aatatggtct
     2461 aaaccgatgg tagtaattgc ggtaatatca ggagtaaata ggttagtagc atccaatctg
     2521 ccgcccaatc ctacttcaat tataaccgta tttaattttg cttctttaaa agcttgaaaa
     2581 gcgatggcag tagtaatttc aaaaaaggaa gcttcatact tttcaaagag gttctcatag
     2641 cgtagataaa tatccagaat tgtttcaaaa gagagatctt ttccatttat ccggaagcgt
     2701 tccgtgtaat taataagatg gggagaagta tttaaaccga ccgttaagtt atgagccaaa
     2761 gccagagctt ccagagttgc acacacactt cctttgccat ttgttccagc tatatgcaaa
     2821 cctcttagac tattctgtgg attgcccata gcttcaagta agttttgcat cctggtcaat
     2881 tcaagcttga cattggcaga gtatttttga aagatatgat cgagaaattc ttgatacaac
     2941 attaggaaat ctataatcct ctactaataa attacccttc tcccagaata tatagagaga
     3001 agggttctaa agattttata gaataaagtt ctatatataa tcagggtgta gttgcttact
     3061 ctgtttcaga aacagtttgg gcaccaggaa cagtacctga agattcaccg gtaagttcac
     3121 ttcttaatgc gatatattca gggtcattaa cacaagcatt aaattcggta tgtttggtat
     3181 tcttccaata attcatataa cgcattagag attcatattt tagtttacca ttagatttgg
     3241 tataggtgtt aatcttgtct atatcgtttg ccataacatt ttgcagttta gttatgctca
     3301 cattatcgta gaatgatttt tctaaaatat gtttggggaa ccgtgccaga tttgggcaaa
     3361 taataatgat accgttataa tgtctggtag ctccttcaaa atcttctgtc acatgtttac
     3421 cgaagactat ataatccacc ttattaccct ggctttcggg gcaatatttt tcttcattaa
     3481 gataaaaata tttacttcct ggctttttag tagttctaag tatatttagg ttaccctgaa
     3541 agtccttatt tgtatccagc atataatcat tagtggcaac ccagatattt ttcatattgg
     3601 ttgtacagtc gtctgtattc ttttgtggat caagattaaa gcttcctgga agagccagaa
     3661 taaaaaccag tgctatggct aatatagcac ttacaatcca gaaaactaca tttccctttt
     3721 gattttttaa catttcaaac tcctgaaaag ttcaaagtta aagttagctt ttttttgatc
     3781 ttgtttatcg tcaagccttt tttgctggag gaattatctt attgatataa tcataggttt
     3841 cctgaatatc tttaaattta taacgataat cagcgtgagg gtctatagac atacctttta
     3901 tcctttgagc cacattatat tcaccccagt tataggcagc cagaaccaga gtccagttgc
     3961 cctgaaaacg ctcttgcaaa tagagaatat acttagcaga tagttttaga ttatacattg
     4021 gaaaatagag ctttcctggt ttatgttttt gatgtatata aaaagctgtt gattctttta
     4081 tctgacccaa acctttagcc ttagaaggag agagtgcaaa attgtaaaat ttactctcag
     4141 tatagattaa atgatagaaa attttagggt ctattttgta gtaaatagca actccgagag
     4201 tcattaatct cagtgttatg ggattataaa ggagaatgaa aaaagacaaa agcaggataa
     4261 agaaggttaa gcaatatttc tttatcctgc tttttgattt tcttctggac atatccagga
     4321 ttagctaata ttctttttgt aatcagctaa ttttataata tgggatattt cttcgttgat
     4381 gatttgatgt aagatatcta tagctttagg ataggcaata ttattactga tggcttgaaa
     4441 atagagtaag gtatcttttt caaagctaat agcataatct attatctcag ttatattttt
     4501 agcttcaatg gcacgacgaa tagcagaatc ttcactgtta aaaatcctac cttccactat
     4561 agtttttaga tataaagcca ctaattccca atcctgggat aattctaagc ttttcaattc
     4621 ggtgtcatct ctcaggctta agaatttctt ttcatgttcc agttcttgat ctctaagcca
     4681 acttatgaac tcatgacttt tgtcatccag gtctttgcgt ttggtggctt cattatagaa
     4741 agcataacca ttccgttcaa tttggacagc catttcaatg atttcgttaa tagaataaac
     4801 agccattttt ttctcctttt aaagtttttt gtctataaat aatataatag attccgctat
     4861 aaaacataaa tatttattgg tctgaaatat ccattataga aggaccaata atctctccta
     4921 caatatccat tgtatcaata ttagaaggtt ggtttttctg gacggtaacc ttctgcgtag
     4981 tttgagcttt ggtgatagaa tccatcaagg ctttttgtct ttcgcggata gggtcttttt
     5041 gggtaatatc cttttcttct ggttgggtta atttttcaac ggtagtagta tcagcagaac
     5101 tggttttaaa tatatcaggc atctgtaacc agccagcatt atgagcatgc cataatattg
     5161 ctcccagaat aacaactatt aatataatac ctataatcca gaagagattt ggagagagta
     5221 aaatcttttt tcccgcctcc tgagtttcag gttccacttt cttaatattc tcaggcatta
     5281 aaaggttaaa tttttgcatc acttcatcta aatctgcttc cagatagcgt gcataattaa
     5341 agaccatagc tcgggcaaaa ccaatataac caatttctgc aaagcgattt tcttccataa
     5401 gtcggacctg ttcttctctc aaacgcaaat cttcataaat ctggtcataa gagatattct
     5461 tttcttctct cagctttttc aggtaagcac ctaaatcttc catatgtaaa ctccattgaa
     5521 aacattggtt actggtcctt caaggaggaa ttctccattc tcaagttttc ggatgagaac
     5581 ttccccaccc ggcatattta ccttaacctc atctgctaag ataccacgat ttatgccagt
     5641 aaagacactt gcaacagcgc ccgttccaca agcttgagtg aatccgacac ctttttccca
     5701 tactttcatt tctatctctt tagcagaata aggatggaca aattctacat taaccggtcc
     5761 tggaaaggaa ggatgttttt ccagaagtgg acctaaacgc tgttcctgtc catttaaaac
     5821 atcatgatag gagacaaaat gcaggtttcc tatatctact aaagtgcctt caattccttc
     5881 tacccaaata ttatccttta ttaaggcagg ttgacccaga ctaacttgaa taccttctgc
     5941 tttgatattg gctctcttaa caccgctatc agtttcaatc tttaattcct ttttacccgt
     6001 atatccagaa agaagagagg cacaacaacg aagagcagaa ccacacattt cagcacgact
     6061 tccatcttta ttataaataa tcattttccc atcagcgacc tcagaagcta aaagtataac
     6121 caatccatcc gctccaactc ccgtgtgatt tttacaaata tctgctgcca gaatatttaa
     6181 tggtagatta gtatttaaag caggaattaa ctccaggatt acgaagtcgt ttccctgagc
     6241 ctccatttta ctaaaaggaa ttaacataaa aggtcttcca tcatctggtt aaaactgtac
     6301 attcctttat gaacggaaat ccattgagct gcttttaaag ctcctgtggc aaaacaagct
     6361 cgggaacgaa catggtgaga aagttctatg gtatcatttt cagaatcaaa aataatgtta
     6421 tgccaaccgg gttctgaacc acctctaaga ctggcaaaat gtagttcgct cttattaata
     6481 ggtctattca agcggtcata tattgccgtt tctttagtgc tgctttgaga aataatagtt
     6541 tcggcaagct gaatagcagt accagaagga ctatctgctt tctgattatg atgcatttcc
     6601 cagccacaga catcgtagat aggaaattta tcgcaaagtg aagcagcata gcgcacaatc
     6661 cgattaaaga tattcattcc aatagaaaaa tttgctccaa agactaaacc gatgttgttt
     6721 gcttccacaa tttttcgcac ttcatcctca tatttattcc aacctgtcgt tcctaccacc
     6781 atatgttttc ctgcggcagc aacttttttg atattatcca gaactgattg gggttgagtg
     6841 aaatcaatac aaacttcacc gagcaggtct tctctgatct ctttttcaca accttcttga
     6901 tgggggtcaa taatagattt taccttgcag ccatgttgtt ctgctaagct ggcaatcatt
     6961 ttccccatct taccataacc gattaaagtg atatttaaca taatatttcc ttataattca
     7021 gcataacgac ggattaacga tttaagaaat tcttttcctt tttgcagttg ttcaatttca
     7081 atttcttcat ttaatgtatg cactttattc attccagtgc cacagataat cataggcaat
     7141 ccactggcat tgataatatt agcatcacta ccgccgccac ctatagtaat ctttggagtc
     7201 aatcctatcc tatgcattgc atctatagct atttgaacca caggttcact ttcctggata
     7261 tgaaatgctt catattccag atttactttc caatcaaagg aagcaccacc aaagtcatat
     7321 tgaaaatgct gtactgctcg ttccacacaa tgacggatat cggaggtaag ctgctccagt
     7381 ttaaagtggt tatgacttcg tgcttcacct ttgacttcaa ctttattggg gacaatattt
     7441 gtggcaacac cgccattgat gagacctata ttactggtgg tttcgtggtc aattcgtcca
     7501 agaggcatag cagtaatggc ttctgctgca acccggatag cattaattcc tttttcgggt
     7561 tccactcctg cgtgagattc tttccccaag aatgttattt gaatagagtt ttgagcggga
     7621 gcaccaataa ccaattcttc cacttgatga gtatccagtg cataacccca ggcagattct
     7681 aatctggact tatcaaaatg tttggcacct aataaaccta cttcttcgga gacagtgatt
     7741 aagacctcaa taggaggatg cttttctcca ctttcaatta tttccttaat agcggtaatg
     7801 agttcggcaa ttccggattt gtcatctgag ccaagtatag tagtcccatc agaatatata
     7861 atgccatctt taactatagg tttaatacct tttcccggtt taacagtatc tgaatgagca
     7921 caaaataaga taggtttctt atttaggttt cctggaattt gagcatagag atttccagcg
     7981 tttccaccag tcaaaatgta agcatcatct tcctcaactt tggctcctaa agcttttaaa
     8041 tctgccttaa gacggtctat tatagctctt tcatctcctg attcactgtc aatagagata
     8101 agttccagga aataattaac tacttcgtct tgcatctctt ctccttaact atgtttcttt
     8161 tattatacat atatttacca ttcgtttttt tgtcaaagct tttagcggaa gattcacaat
     8221 aagatagccc tggataaata tagtataact ccctttagtt acccaggatg gtggattata
     8281 taattttagt actataaaag aggaagagca gtcagacttt tttcaagttt atgctggata
     8341 aaatttgatt tgtagatgag aagaaacaga taaaagagag aatatttgta atataaagaa
     8401 tccaggaaga ttaccacagc ttcacgatat ttcttgacat tagtatatat aagaaggaaa
     8461 ttgtaagcta tgaataaatc catactctat acctgcgaat tgaaacatca tcgtcaagtg
     8521 gcagctccac tatgttcagt ttgtcctcgt gtggcaaaat gtcgctcttt ccgtaattgg
     8581 tttgacattt atcgtgatga gtatctggat tttgtaatca acatttgtaa taaattccct
     8641 gaaaaatata ccctggaggt aaccctaatg gctgaaaagc aaacctttgt ccaaatagtt
     8701 gatatgtcaa cgggcatgat tgaaaagata gtgaacctga aggaaattga agccttaacc
     8761 ccggaagaga aattggaact tgcccgtaat aagaatctct ttatagtcac acatcgcata
     8821 gtgccaattg tgcgagttga aatgaagaaa actgtcattg aagagcctat gcaatttggt
     8881 ggcaacaatg aaaaaaagcg gcaagaagaa gcggagccta tagttgagcc cgtttctcct
     8941 gctaaaacta agaagaaaaa atagacctta gttaaatagt tgaaaaaaat ccttttcaga
     9001 aaagcaaaat tctaaatact ggtttattta tgaggaatgt agttaaattt gagagctact
     9061 agatgctcca gaaccttaaa accaaaaagg agagtttatg tttaagcttg cagatgaaca
     9121 tttagaagta gcacagaaaa ttgctcaaaa acatcgtcgc aaaaaacatt gtgaccattg
     9181 ctatgaccgt ggctggattg gcattaatga gcaaaatctg ctggtgctct gtcctcattg
     9241 tgtagaccgc gaggctgctc tggaagaatg gaaaacctat gtttctgaac atgaagattt
     9301 aaaagagcat ttcagcgaat tatttgagga aaaacctgtg gaagaagaag aaactgaatc
     9361 tactattcct gtggcacata ctaagaaacc cgctcaacct caggttaaga aaacctttgt
     9421 tcctggacag aaaaagtttg gacgaactaa aaagattggt taaaaatatc agttaaagag
     9481 attaatttgg ataaggtcat ttagattgta aaggtgatat gtataaaaat atagatttaa
     9541 aagcaactcc tcaggattta gaaggaagca tccgtcaata ctggaagcag cataatttat
     9601 tgcaaaagag tgtacttttc cgggaaggtg ctcctaattt tatcttttat gaaggacctc
     9661 ctactgctaa tggaactcct ggaatccatc atgtaatggc aagaacctta aaggatgtgg
     9721 tctgccgtta taaaactatg actggttttc aagtaaagag aaaagcagga tgggacactc
     9781 atggattacc ggttgagatt gaagtggaaa aacaactcgg tttggaaagt aaaaaggcta
     9841 ttgaagatta tggcatagaa aagttctgtc aggcttgtaa ggaatctgta tggaattatt
     9901 tagacctctg gactaaaatg acagaattga tgggttactg gattgacctg gaacacccct
     9961 atgtcactat gtacaatgag tatatagaat ctgtctggta tattttaaat gatttcttta
    10021 aacggggttt actctataaa gctcataaaa ttgtgcctta ttgtccaagt tgtggcactc
    10081 ctctttcctc tcacgaagta gctcagggtt ataaagaagt ggaagaccct tctatttata
    10141 tcaaattcaa agccagagaa gaagaaaata cctggtatct tgcctggact actacaccct
    10201 ggactttgat ttctaatgtg gctctggcag ttcatcccga tttcacttat gttaaagtgt
    10261 ttcataaagg tgaatattat atactggcaa aagaacgttt ggatgtgctg gatagtgaat
    10321 atgaaattat cagtgagttc tctggacgag aattagaata caaacaatat gagcctttgt
    10381 ttaactttgt accggtagat aaacctgcct ggtatatagg tttagcagat tatgtttcta
    10441 tggaagaagg gacagggatt gttcacacag cacctgcttt tggacaggat gattatcaat
    10501 tagggaagaa atataacctt cctttggttc aaccagttga tgcggaaggg aaatttacct
    10561 ctccagtaga gccctgggca ggaatatttg ttaaagcagc agataaagat attattcgtt
    10621 tcctgaaaga aaagggaaat ctatataaaa gagaacaggt taaacatagt tatcctcatt
    10681 gctggcgttg tcaaagtcct ctcatctatt atgccagaga cacctggtat attcgtacct
    10741 ctgatttcaa ggaacagcta ttagaaaata accggcaaat ccgttggtat cctccttttg
    10801 ttggagaaaa acgctttggt gagtggctgg aaaataatgt tgattgggct ctttcaagag
    10861 atagattctg gggcactcct ttaaatatat ggatttgtaa tgattgtgga gcaaggactt
    10921 ctataggttc cattcaagaa ctgagagata aggggacttt aaaaaatggt ttgccaatac
    10981 ctgaaaacct ggagctccat cgtccttata ttgatgaggt cgtgctaaaa tgtgaacatt
    11041 gtggtggaac aatgattcgc actcccgaag tgatagattg ctggtttgat agtggttcta
    11101 tgccttttgc tcaatggcat tatccctttg aaaatgctga taactttgat aaagagctat
    11161 ttcctgcgga ttttatctca gaaggaatag atcaaacccg gggatggttt tattctcttc
    11221 ttgccatctc aaccttattt aaaggcgttt ccagctataa aagttgcctg gtgaatgaga
    11281 tgattctgga taaaaatgga caaaaaatga gtaagtccaa aggaaatgct gttgacccta
    11341 ttgagttgat gcaggagtat ggagcagacg caattcgctg gtatctgctg gaagttagtc
    11401 ctccttgggt tcccactcgt tttgatgtag atggagttag agaaattgta ggtaagttta
    11461 ttggtacctt gaagaatact tattccttct ttgctactta tgccaatatt gacggatatg
    11521 atgcatcaag ctattctctt gattggcaaa ggattgctga actggatcgc tggattatct
    11581 cgcgtctaca tagccttata aataaagtgc gtgaatggat ggaagaatat gagttaacca
    11641 gagcagttcg tgccatacag gattttgtta tagatgaact ttccaattgg tatgttcgta
    11701 ggagcagaag aaggttctgg gcagcaggga tgacctcaga taagattgat gcttataaca
    11761 ctctttatca ggtgttagta acggttgcac aattggttgc tccctttgca ccatatcttt
    11821 ctgaagaact ttttatcaat cttacaggca aagaaagtgt tcatctaact gattatcctc
    11881 tctctcatca ggaagttatt gatttagaac tggaaaataa aatgcaggta gtgattgatt
    11941 tggtttcctt aggtagagca gcaagaaatg cctgccagat aaaaattcgt cagcctcttg
    12001 aaaagatgta tttgcctgct aaatataaag atatagtaga atctatgcga gagcttatcc
    12061 tggaagaagt taatatccat aatctggtct atgtttctga agaggatgac tttgtgcagt
    12121 atgaggttaa acctcagttc aagattatgg gtcctaaata tgggaagcaa atgaaagcga
    12181 taactgaagc tctcagtaag cttaaaggtc aggaagtgct ttctgccttt aattcttctg
    12241 gagtttatca tcttacagat ttggggattg accttgtgcc cgaggatgtt gtggtgcaaa
    12301 ttattccccg ggaaggcttt gtctttgaga gtatgaatga taaatttgtt gccctggata
    12361 ctactttaac ccctgatttg cttcaggaag gatatgccag agaattggtt aataagattc
    12421 aatttaccag aaaagagcag gattttgata tactggatag aattgtggta gaatggtatg
    12481 gagatgatga tattcaggca gctatagata aatataatga ctacattaag aaggaaactc
    12541 taagtgacga actgcgaaga gttaattcct ctcagaatat gcaagtttat gatataaacg
    12601 gaaaggaagt ttatttaaag atatataagg tggaaaataa atgaatgaag ggcaaaacag
    12661 tgggatgaat aaactctttt gtagcaattg cggaaagaaa attccaatta atgctaagtt
    12721 ctgtagttac tgtgggcaaa aaattgaagg ctgggaagaa catccatcgc aggaagataa
    12781 aattaaaccg cagaaaaagg ttattaagcg agagccagaa agtgaagata tgtcttttgt
    12841 ggcttatcag actttagaac ctgggacatc cttctataat tacaaagtta tcaaacttca
    12901 aggaaaagat actgatggag tgcactatct ggtggaaaaa gaaggtaaac aatatgtact
    12961 gaagctattt ttcccctata aatactctaa tttagaaaat atcattaccc tccaaaactg
    13021 gctgaataga gttcaaaaac ttgagaatcc ttatatagtt catgtagaag aaatcaatta
    13081 caataacgac ccagtatata ttgtgacaga aattgtggaa ggaatttccc tatcaaagat
    13141 taagacttat aatcctgaac tattaacgga atctctggtc agggagattg ctaaacaact
    13201 cgtttcagcc gcaatggaca ttcatcagtt gggactgagt attaaaaacc ttacgctaaa
    13261 tgacttaatt ttaaatccag agactaatac aatcaccatt ttgacttctg gtattagtta
    13321 tgagacaaca gatgagcgag atgaagtttt tcatctgggc gttttgcttg ctcagatgct
    13381 ttctgccaat caattggcgg aaacggtcta taatgatgaa attttaagcg aacataaatt
    13441 tacttatatc cctggtatta cattaagtat gaataaagta ctgggagaat gtttacatcg
    13501 caatgtcttg cagagatata atactttgcg tgatgtgctg aagggtctga atgccttaat
    13561 tgacatagaa aaagatgaaa tatatgaact gtcaggcaat aaattgcaac cagaagatat
    13621 atcacctatg caagtgcctg aaccaaagac aagtaaagat cggattatca ttactgccat
    13681 agttatcggt gttgcagcaa tagttatctt ctttgccata aattggtcaa aaataacgaa
    13741 agataacggt acttccagca cctggatttc ttctataggt gaagaggata ccatcaataa
    13801 tgaatatcag aataaaacta ttttcaatca tagagacact ttgaaaaacc aaaactataa
    13861 tactatagac tcacgagtag atccccgtaa ggctatttta tctcagaatc aatcttccta
    13921 ttctggttca tctggtagta cggttaaaat tgatacttca cctgatagag acctttttgt
    13981 atatgttcca gacggcactt ttggttttac ccgtttaaag gaaaatccta atcataatgt
    14041 tgtgcaaaat ggcttttata tggggaaata tgaggtcact caaaaagagt ggaatcaata
    14101 tatgaaacca gctaatgtaa gttttgtagg agataactta cccgttgata atgttagctg
    14161 gatgaatatt gttcgttatt gtaatgctcg tagtgaggca gaaggtctaa cacctgctta
    14221 taccatttta ggaaacaatg ccagttctgt cacctgtaat ttcaaagcaa atggttatcg
    14281 tttacctact gaagcggaat gggaaatggc agctaaaggt ggacaattat atacttatag
    14341 tggttccgat gatgcagatg aggttgcctg gtatagagaa aacagcggaa gaagatatag
    14401 gactgttggt tatcagaaaa agcctaatgc ttatggaatt tatgatatga ccggaaatgt
    14461 agcagaatgg tgctgggact ggtattctga agattatccc tcttctctaa ctgagtttgt
    14521 taatccaaga ggtcctgcat ctggaacttt aaaagttatt cggggtggca gtgttaacaa
    14581 tggattgggg actaatttaa gtgtggttta ccgaactaaa ggtgatcctg ctaaaggtta
    14641 tccttttgta ggatttcgtt tagttcgcac ccattaagtt tatttgcaca acaccatttt
    14701 ctggaactgt ttatctttac ctccagagag gacacagaaa taaattccgc tactaacatc
    14761 tttaccacta ttatcttttc cattccagat tacctgataa gctccagcat ttttagtgca
    14821 tttttccagt tgacgaacca tttgtccttt tgtattatat atatttaaag ttatatttgt
    14881 aggttctgct aagctataac ggatagtggt ttcagggtta aatggattgg gataaggggg
    14941 ataaaggaca aagcgaggga tagcaggtaa actgggatca caattaggag tagcagttcc
    15001 attaataatc aggatattat caatagtcca accagggtca gttaaatcta tatctgttga
    15061 attatcagtt aaatggaagc ggagatagat attctgtcct tgaaagctat ctaatgagac
    15121 catttctttc tggaaataac taagttctcc cgatttttcc cagatgcatt cccagttcaa
    15181 attatccgtt gagatttcca ctcttgcagg gtcaaattga tattctgtat atagatggga
    15241 gtcaaagatt agcatcgtgg aagcctctat tggaatatag ataggttgag ccgttttaat
    15301 ccagacatca caattcatag catagaatcc atttccaccc cagctatctg ttatagcata
    15361 gccggaaacc gacagagtat tttcacgcac ccagggacca tttatttccc atctatcagt
    15421 tccattttcc caatcttcac tgaaaagaat atttacagta ctaagttcta tcgttaaata
    15481 attttctcca gggctcaaag tcactgtacc attccagggg aaatagcctt ccgcataaat
    15541 ttctacagga tattctcctt cgtaaccatg aaaaataaaa ttgccattaa ccatcagagt
    15601 atctgggaaa acatcgccaa taataatgcg agcattgata ttttcaccgt tggcattgat
    15661 gtttccgttt agaattgccg gttcgcgggg ctgtaaatat acttgtattt gcgtccagtt
    15721 actattttgt accatttgac tggggataat ggtatcccaa tagccttttt ttctaacctg
    15781 aatcgtataa actccagaag ctaaaggacg gtaaaatctt cccgtagttg ggttggaagt
    15841 acgaggtcta aaccagggag cattatgttg ttggatgatt atttctgctt ccagaggttc
    15901 attagtaacg gcatctctaa tttttcctgt taacatagaa ttgcttgata ctgcttcaga
    15961 agagggtagg gctctattga ttaaccagcg aacaccattg ctacatcttt gaactgtatt
    16021 taccatcagc gtggaatcgg gttgtatatt actggttcca cattctatca aaacttgaaa
    16081 agttccatat tgttgataca tccaatcatg gaatgcaccc tgacgactaa gattggggaa
    16141 ataatcatag gttcctgttc cggtttcttt aattatgtga gaagctattc cactggctat
    16201 agaggcagaa aaggctaaat cgggtgaagg tcttacattc ttccaattaa atgaataata
    16261 acacttttca cttaggttgc cggtacgaga ggaatgccaa cagatggaat aaatgaattt
    16321 ataacggtct gctaattctt ttatggcttg attttcactt tcactcatag gagcgggacc
    16381 tctataataa tcccagactt ctaaacctcc aggttggaat aaggtgtctc catgcaccca
    16441 gttaaaacta aaattcctgt ttatatcaac tccatctaca tcatatccca cctgaggact
    16501 gaaatcaaaa attccattca tattattgtc tctcttattc ttacggtagg aagagtcaag
    16561 attggaggtc actacattat gtccttcagg attaagtgtg gggacaaacc ataagtcaag
    16621 ctgatttatc cattgcatat agggaagctg atatctatta gttaaaatct cagcaatatt
    16681 gctcatcgta atctgaactc ccagaacttc ttccgcatga acttgtccta caaaaagcaa
    16741 ggctggctct tcttcatcct gattaacatt atctgtgatg cgcatagcat agatggggat
    16801 atgatcttct tgagaatagc caatgagtac ttttttggct atatcaggat attgactttc
    16861 ataggtagaa agcagttgag atatctgatc gtaagtataa taacaggaag gtagtgcttg
    16921 aatctttata gtcagaacag taaaaaatat taatatgaaa ggaagatatc tcttctgcat
    16981 tttaatatcc tttttaacct ttaataatgc aaattataag ccaattattt tattcatttt
    17041 acaacctgaa taaatataat acctgaattt ttaagaaaaa gtagttatat tttgcaattt
    17101 ataagcaatt aaataaaaat ctttcagttt tatcccaaca tctctttcaa tctattcgct
    17161 aaattatgta cttgcttact cttttccatt tcccagggaa tttgatagtt atgctctgtt
    17221 tccgaaaaaa gtctttttag gctatgataa atagtatctg cagattccat cgcacagata
    17281 taattatgtc cacattcacg aagaagttct gcagcttcac cgtaataagg aaccattgcc
    17341 aggataggtt tttgtgttct taaatattca cccaccttag aagttaatgt gccataggga
    17401 ctgctactat tgataaccag caacagtaca tcagaagcca tcatattagc cagagcctct
    17461 ctatgttcca gaggaggaat gatttctata agattagtga taccgctttg agttatttct
    17521 tctctggtct ccttaaaata atttccgaat agtcgcacct taatattctg agggattttt
    17581 ccctcttcaa gcagacgatg tagagcttta taaaaagcgt tcatattgcg acgagcatag
    17641 atacttccaa aataagaaaa ggtatattca gaaggtttag ggacagcagg taaatcaata
    17701 aaatcatttt catcccagcc attatagacg gttagagtct tttcagcaaa ttcgggaccg
    17761 aaggctttaa tgatgtctct ctctataccc tgtgtagcgg taactatcaa actggcatag
    17821 gaatagaatc gcttttccca gttccaggcg tattttcgat gaagggtggt agcttgtaaa
    17881 ttatagtccg acagtagatt ccagtaatct cgcatatcta aaaccagagg aatgccactt
    17941 tctttagaaa gcttatatgc accaactcca gaagaaaaag gtcccagaga aatgtaaatc
    18001 aagtcataat gagtttcaga tatagctttt ctacctgctt taataagaaa aggattccag
    18061 ccaatttttt cgtctaaggg ataaagcctt ctaatccaga gctttatatt ctctggagtg
    18121 ttcatataaa gtcccgttgc ttgattacct ttactgatac cagtcttacg gaataatgcc
    18181 ataggatcaa gagaatcagt ccgtattaat ctttgttcac tgcattctga aagtaaagaa
    18241 aagtcacgct gaatgtattc aatatcgcta acggttataa catcacagct gattccttct
    18301 tgagcgaggt atttaactac cttgacattt cgtaaagaag caggacctcc caggggagga
    18361 taaaaatagc tgatgtaaag tatatgcatt ttatttagac gcaagatttt ggattaaatc
    18421 cagtaattct ggttcaactg attcccaatt atatttattt tctattaaat caatacagcg
    18481 tttactcatt tcttgatatt tatcctcgtc caattttaat atacgagaaa tagcctttgc
    18541 tatggaagca gagtggtatt cgcaacaata accgagttga tttttttcta tcaaatcaga
    18601 cattaatggt gttctggttg aaaccacagg taaaccagca gcaaaatatt ccagaacctt
    18661 taaagggaga gctttgctca atcttttatt ttgagcattg aagagccata gtccaatctt
    18721 acttcttttc aatagcaatc caattttttc aggtggaacc cagttattat aatagataaa
    18781 agccgttaaa ttatattcag tgagcatagc atcaaatttt ttttccactt ctggtgatgt
    18841 aaatttccca acaatcaagg cttttaactg gggaaattca tctttgagta tattgatact
    18901 tttcagtatc ttgtaagcac cacggtctgg agtcaaacca ccaatatata tcaaatcaaa
    18961 attcattgtg caaatattgc tcaattccat aggaacttca cagctatcat cccatacatt
    19021 tttaactgga taattcggaa taactgcaac ttctcctttt ttaggtgccc tgccaagttg
    19081 ttgtaaaatc tgttcattaa cggcaatagt tccatcaacc ttacattgca ggctgcgttc
    19141 taaaccgaga taaatgctct tcattatcca tttaaaaggc agtggaaatt tttctgaaaa
    19201 ggcatcagga taaaattcat gtatatcaaa aaccactttg cactttattt ttttctttaa
    19261 tgccagtcct accaaaagag ttaaaggttc aacacagatt accacatcag gatgaatttt
    19321 tttagccttt ttatacaatt gaaaaagaat accccattta gaatccgctt cagtaacatc
    19381 ctgataggga ttgaccaggg aattgcaaac tccactggca catataatat ggacttcacc
    19441 aattttagcc agacttcttc ccagcttgta ataaagcctt acttcattgg taggatgagc
    19501 gttggataag atgcatattt tcataattct ttctttatat gtgaaaaact aatatgtcag
    19561 taaatgcttc aatgtcaaga attattttaa atttaagtcc attctttgtt tacttttacc
    19621 taattaaatg ataactggag cttatgtccc aatatctata attaactgat tttaaaggag
    19681 ataaattgaa tatctcctat ccctcaccta ctatcaagtg ttaatcactt cataatcaag
    19741 cgttaatata ataagacttg attaacgctt gattaagact tgacagctga ttgttttaaa
    19801 ggaagagaca gagcttaaac aggataattc tctcgtatat atgaagtaat aaaaacgcta
    19861 tatcattcat agcggcttat ttgcagaaaa aaaggattga cagtatgaag ggaaactatt
    19921 actaattctc ttagaatatt aaggagaaaa tcctatgagt tcaaaaaaaa taaatcgtcc
    19981 taccttgagc tttagtaatg ataaaaaaag ggagcgaaag gtttcagata cgcagaaaga
    20041 aagtaaggat aacgaagaga aaaagaaaaa taaggaaaaa tatccggaca attctgttct
    20101 ccaatggtgg tcttccattt attttgacta tttaaaacag gaagcgaata atcccgataa
    20161 tcctcatcct ttgatggctt ctgtggaagt agaagtggtt aaagaagcag atttagagca
    20221 gatattagcc ttatataaac aggcaggttg gtggaatgaa cccattgatg aaaagtcata
    20281 tgagcgagtg agaagaatca tatccgatac cttttgtttt gtgatagcac gctatcagga
    20341 tagaattgtg ggaatgggaa ggtctatatc ggatggagtt agcgatgcat atattcagga
    20401 tgtggtggta gataaggatt ttagaagaca gggtattggt gaagctatca tacatcgtat
    20461 agttcaattt cttttagaac ataatataag atggattgca ttgatttctg aacccggctc
    20521 agaagagttt tatcgccaaa ttggatttag ggatatgatg ggttataccc cattattttt
    20581 gcctattgaa aatatataat aatctttatg ttggaattaa cacccttaac acctgctcat
    20641 attccactgt tacaaaagta tttaagagct tatcctcgtc aatcttgtga ttatgctatt
    20701 tgtaatctta tgacctgggg aaagatttat gggaacagtt ttactatctg gaaagagcat
    20761 ctcgtaattg ttaatccgaa atatgattat gttatgtatc ctgtgggtcc tggattaagt
    20821 gcaaaagaat taagggaatt ggtagatatt tatcgggaag ggcatcccct cactcaactg
    20881 gtgctaattc cagaggattg gcaaattaag acaccagata taacgcaata ttttcagctt
    20941 agagaagata gagactgggc agattatgtt tatttaattg aaaatctggt caagcttagt
    21001 gggaaaaaat tagctaaaaa gaaaaatctt atttctcaat ttattcgcac ttacccggga
    21061 tatcaggtta ttcctattac ttctgataaa aaagatgtca ttatccgttt cagtgaaaag
    21121 tggcgtagag aacgcaatat ggaaggtatt tatttaaatg cagaatttca agcgataaaa
    21181 aatacgatgg aaatgtggga tgagatacca gttgaaggga taataatttg ctataaaaac
    21241 cgcatttctg ctttttctat ttttagtgaa cagacggttg atatggtaac ggaacacttt
    21301 gagaaatttg atcctgagat gaaaggtagt gctcaagtta taaattggga gaccgcaaaa
    21361 tatttacaag accgttatat ctggttaaat agagaacagg atatgggctt aaaagggcta
    21421 agacaagcta aaagtagtta tatgccagcc tttctggtaa aatttatttt tggtaagcca
    21481 ctggattgaa atagatttaa gcgatttttt gtcgccgtct aaacatcaga ctgataagat
    21541 ataacacgaa ttctaataag acaatattcg ctccggttgg aatgtctaac caccaggaaa
    21601 gtgcaaaccc accacttgct gctaaaatag caataatagc gctacagagg atagcatagt
    21661 ctaatctctt caccagattg aaagaagtaa ccgcaggaag aattaattga gcgctgacca
    21721 aaattatccc tgcagctctt aaggttatga caatattggc agctaataga atcataaata
    21781 tggtatttac taatcccaca ggtaggcgat agaattttgc catctcggca ttatatgaaa
    21841 gatagaaaag ctctttataa aagaaagaaa tgaagaaaat atccagtaac gccagaataa
    21901 ctaattgata aatttccgat ggtgatacca gcaagacatt accaaagaga taactttcca
    21961 ggtcaaaact ataacctttt tttaaagaga tgagaataat acccagagcc atagaaacac
    22021 tgagaaagat agttatcgta ttggcttctt gcaatttatg tttttgggaa agccagctta
    22081 ttgccaacgc aacgataact acaaaaatta gagtggttat ctgcagattc agttccagca
    22141 gtaaagccag tgcaatgcca gcaaaagcta tgtgagaaag agcttctccc atataggaca
    22201 cctgacgcaa agttaccaat ggagaaaaaa gtgagaggga aaaagatgat aagagtgcag
    22261 ctaaaattgc atgatagata aaattataca tcaatagtcc ttttcaatta gacgcatggt
    22321 atctccaaag gttttatgaa tgatttctgc attgaccagt tcggcactgg tgtgacaatg
    22381 caattttcta ttcaggcaaa tgagaaaggt gcaatattcc gaaagaatat taaggtcatg
    22441 agagatagtt aaaattgtct ttcctgcttt atttaagtct tttaggagaa taaaaagact
    22501 ttgcacgcca ggctgatcca gcgatgcctc tggttcatcc agaataagat atttactatc
    22561 agaaatgaga gctcttgcca gaaacacacg ttgcaattca cctccggaaa gagaacctat
    22621 ttgatgatta gccagatgtg caattccagt aagtgataag gcttgcatag ctcttttacg
    22681 atgaatggat ttaaatcttg aacccggtgg caattctgct actaatccca taagtacaat
    22741 atccaaagca gtggcaggaa aatggcgatt aaattcttca tgttgaggaa gataaccaat
    22801 aggatgctct tttagccatt cccaatgcgg gatgccatca atataaatag agcctgtttg
    22861 aagaggtaaa aatcccaaaa tcaattttat taaagttgat ttgcctgcac cattgggacc
    22921 aattaaagcg gcgaactctc cttccgcgag ggtcaaattt atgtcttcta atatgatatt
    22981 tccattaatt ttataggaaa ggctacgaat ttctatcatt taatcagagg tctaactctt
    23041 accaggcttg cttcattcta ttccagttta taagaataac ttcaggaagg gattgagcat
    23101 taagagtctg tcctaaaggg tccagctcta ttacttgtaa atgaaattct ttagccagaa
    23161 cattagcgga tttgcggtcc atttgaggct ctacaaagat ggcttttacc tgattagctt
    23221 taatcaagtc tcctaaatat accagttctt ttggagttgg ttcttgacca ggagagcttt
    23281 gaatacttcc taaatagtcc agcttataat cctggataaa ataatggaaa ctatcgtgat
    23341 aggtgataat tggtgtgtga gaaaactgtg ctctttcttg aataatgaga ctatccagac
    23401 tgctgaactg agatataagc tctctacagt ggaaagatat gctatcttta tagctcggaa
    23461 atgttctttg caatggttct tccagttcag ataccagttt tttcattaat cttggtgaaa
    23521 gccatatatg tggattagga ttttcctctt tttgaggcag ttggagtaat tgttcggcag
    23581 ataaatgttt ctcgcccagt gcatttaaag ctgtctgaat attggtttct aaaccaaaac
    23641 cattactgaa cactaagtct gcgtcatata aggattttaa ttctgctgga ctgggattat
    23701 aggtatgagg agaaaaattt ggaggaataa gggttctcac ctctatttcc ggtcccacca
    23761 tctgttttaa gagcaattca tagggataga tagagacaac aataagatta gatggtgaat
    23821 ctaccgcctt tttacaagaa ttaagtgcga acagagagct tataatcagg atagagatga
    23881 gaacctttaa tttcattcca gctcacgaag caattgggtg agtaatctaa cacccacgcc
    23941 ggttgcacca taactataaa taccttgctc tttatctctt aaagcggtac cagcaatatc
    24001 cagatgaatc cagggtttac ctgctacaaa ttcctgcaag aaaagagctg aggttatagt
    24061 tcctgctaaa ggaccaccgg tattcttaag gtctgccact tcagatttta tgagttcctt
    24121 atagggttca taaagtggga gttgccagat tttttcaccg gtaaattcag aagctgattg
    24181 taatttgttg agtaaagcct cattatttgc taaaaccgca gatatttgat ttccgaaagt
    24241 agcagctgcg gcaccagtta aagtggcaat atctattacc tcgtctatgt gttctctact
    24301 tatggcataa tggattgcat caatgagagt taagcgacct tctgcatcag tattgaccac
    24361 ttcaatagtt ttacctgcca tactgcgaat aatatcaccg gcatgataag cagagccaga
    24421 aatcatattt tcacaggcag cgacaattcc aatgacatta acctttagtt ttagattggt
    24481 gattgcagcc atagtaccga taactgcagc agcaccactc atatcatttt tcatattaac
    24541 cataccctgg caatttttaa ggcaatagcc accgctatca taggttaaac ccttaccaat
    24601 gagaccgata gtctttgctt tagggtcggg atttccatga taggacatca gaatcagacg
    24661 aggttctttt ttagaccctt ttcctactgc cagaaaagct tccattttga gccttttaat
    24721 cttatccaat ccaaatactt caatagagaa gccatatttg agagcgactt ttttagctgt
    24781 ttcagctaaa gattcagggt agatgacatt agcaggttca ttaaccaggt cacgagccag
    24841 atttgttgct tcagcaaaga ttctaccttc cagcacacca cgattgaaca tacgtggatt
    24901 tttaaggtcc atagctaaat gaatagcttc cagagatgga ggttttgttt cactaagata
    24961 tttatcaaag cggtagccag taagcaaagc agcttctgcc agtatatgtc caaaatgaag
    25021 ttcacttaaa ggatttttat aaccggtgaa aatataagct tcttttgctt ccagttttaa
    25081 ggcttcacgt attgagatag caataagatg gcgaaaacgg tcattatcca gttccttagg
    25141 attgccaata ccgcagagga taacattggt tcgttttctg gaacgaataa aagggaagct
    25201 cttaaggctg gcatatttga actgaaagtc gtcctctttg actactattt tgatagcatt
    25261 ctgaaggtat tgagggagat attccagctt ttctatgtct ccattttctt cctgaagtat
    25321 aatcaccgtt tccatatatt cagttggctt atgcaacaag tctattttca tttaggtctc
    25381 ctatttatgt tttatattaa aaatcttcgc ttaaacttaa gtaataagtc ggttttgaaa
    25441 tgtgcgagag gtctgttaac caggtaatat ccaggctaag gataaaaatg cctaaattta
    25501 tccgcggtcc aaatccataa cccagataga ggtctttaag tttgccttca tgagtagcct
    25561 ggaatttatt aaagtcatca aagacagttc ctatttcagc aaagaggctt ccgcgtacat
    25621 taggtatatt taaaggtaaa gggaaagcga tatttatatt ttcaaagaag ggaaatctca
    25681 gttcagtgca gaaaactgct ttcttttcac cgctaagatt tccatcataa gcacgaatac
    25741 cataataacc accgagagca aatctttgag gataatctcc tacacttatt ccagtgataa
    25801 gacgcgtagc taaagaatag cgtttactaa ataatatata atttcgccaa tccaggtagt
    25861 tggttagata atcaagatta ttatctctta aagaagtgcc aacagaatag tataaacgtc
    25921 tacctgcaag aggaccagtc ggaccatata atgcattatc atgaaccaga tttacagctg
    25981 gactaaagac cttatcactt ccgagatttt cttcttctgt ccaatcacca atattccagt
    26041 cccaataaga aagataatat tcttgttgat agaacatatt ttccaattca atacgagtaa
    26101 agcgattgaa gggatagcgt aagagaagat atagacctgt ctgtctttgt ttataacgaa
    26161 tatacaaatc attttgtgga ccgggaatac tcatctgaat attgattagg tcattgagat
    26221 taaaaagtcc aataccataa tccattcttt gttttagata tagatagctt ataagcagat
    26281 ttgaatcttt aagttttccc gcagcgctta agctaatccc aattccatga tttcccataa
    26341 tatcacttaa acttaattcc agataaccca ctgttcctgc ataagatgaa taagcaagac
    26401 ctccccagag ggtatctaag gaaaatttag ttttgtaagg tttgatttct gggatagtct
    26461 caatcgtgat cggcttgtca tcataactaa ggttacgata cattctcaag gaatcagaag
    26521 aacccatctc ttcgttggaa tgggcagatt ctattaaggg gtctttttgc atataaggac
    26581 gctggggtct tttaccgtag taattgagtc tggagaggtc aacggtctcc aataagtcat
    26641 ttttcaactt aacaaactga ggtggataat agtcaataaa acttagattt tctgtctgta
    26701 tttcagtgaa atatatattc caggcatcct ggaaatagtt agaaagaaca atttgtttac
    26761 cattaggaga aatatcagcg gaataaactc cagctaaaac attggtaacc tttgcttttt
    26821 gagcagaggt tagattaagc ataaataaat tactaactcc atctattgag ctcacaaaat
    26881 agagttttgt gccgctggca tccagagatg gataaccgca atcttttgtt tcaagggtca
    26941 attgagtgca gattccatta tttaaattca gatgaaagat attttgattt agatgcgaaa
    27001 agaggcgatt agggaattct ggttctttct gacttctttc cgaagcaaag acaatgctat
    27061 taccgtcagg tgcaaaacgc ggttgcaggt cattataata atcatctgta agctgttgta
    27121 gttcttttgt ttccagatta taaagataga ggtcacattg catatttttt tgtccagcaa
    27181 agacaatgct tttaccatcg ggagcaacat ccaattcata tatagcatca aagtccttaa
    27241 gtttgatggt ctggacaatt ttctttttgc tggtatttac gatatagata caatcaccat
    27301 cactggtctt tgcagaaaaa gctatccttg tattatcagg aaaccagctc agactggaac
    27361 ggaagtaata gaattcttcc atctttccag agacctcacc tttgataatc ctttcgggag
    27421 cacttaatcc gtgtaatcct cccatccaga tgctataacg agctccagca gtactgaaat
    27481 agacatattt atcaccttga ggagaaaaac ggggcataag attaaaatag gaaccgtctc
    27541 ttttgctatt agtgcgttgt tccagagata aagagggtag gtcatgggtt tctattaagg
    27601 gtaaaaattc tcgcttcagc tgaaaacgcc agcgggactc caattcttta aaatccaagc
    27661 caaagatttt tttagtggca taatctatac cactggtaga gcgtagagca aagaaatatt
    27721 ccatcaattt atcgggacca taggtctcag caataaaagt aagaaaactt tctcccagac
    27781 gatatgcata ataaccactg gtgttttcta tatagggcaa tttgtcattt attaccatat
    27841 ccaggatgaa catattatta taatctttct tgccgagagt ggaaaaatac tcaggtagcc
    27901 cctctgaaaa ccagaaagga atataggaag aataaagtga agcaagcgat tcctctttag
    27961 ctctttccag ggcattgaga taggcatgag ttagttcatg gattaatagt tcttctaaat
    28021 tggcataact tccatcaaaa ggtataacta cacggttatg taaactttcc gtgaaaccac
    28081 ttacttcttc agtgagaaga ggataaataa tattagtgtt ttgaaaagcc gctttactac
    28141 gatagaagat gataggaatt cttgaggtga taggaaattt aagctgttct ttgagataat
    28201 agtaggattc ttctgccatt aaagcggcaa gtttgccaaa ttcatcctct ccttgcggaa
    28261 aatagatatc aaagtgcatt gtctggatgg tagaccattc ctcaattttg gcattaacct
    28321 tattctgtcc aaagtagtat gcctgaacga aagttaccag aattaacagg gctaaagtga
    28381 gatagtgctt aagcttcaat ctttttacct taaaatatct ttatattgat gtatttctta
    28441 ggattagtgc ggatatcttg aaaaagtgaa tccagactgg tagtggtctc taataatctt
    28501 ttataaagtg tttcatcttt taggaatttt cctgcggaac ctttaccttc attcaaatcg
    28561 ttgagggtat tatgcaattg agtggaaagc acttgcagac tatctaatgt attatttatg
    28621 tttgcaatag cttcaggggc aagggtcaaa gtggaatcaa gatgggcagc attattatcc
    28681 agaagatatt gtagtttttt actcagttgt tctattttgg ttaaggtatt attcaaattg
    28741 gttctggttt catttatcat tatctctgca gaaagtactg cagaatcagc atcatccaga
    28801 agggtggtaa ttttatctgt aaagattttt cccccttgta attcatttaa tgcttcattg
    28861 actttagcga tagcgatgct tacttcatca aaagcattca taattccgag aggtttatct
    28921 cctttctgaa tcttacgcag gtcaaggtta tgttctccat tacctggaat aatatttaaa
    28981 gcggttccac ccataatgct ggtatctgta atctggaatt tagtcccttc ttgtaacttg
    29041 atatctctat ttatatttgc agtaaccagg attttatcag gctgaagttc tatatactta
    29101 actcttccaa cttccattcc tctatagaga actgggtcac ctaaatccaa tcccataaca
    29161 tcatcaaaac aaactttcaa ttgctgttgg gacctattcg taattttact acttaaccat
    29221 aaatagccaa agataaggat agccaggatg ataatggtaa agagcccgac ttttacttgc
    29281 gttttgtgga aattaggata gaatcttttc ataatttatg tctttaattg ctttttttct
    29341 gctgctggga agaggatatt atttagaatt aatctataac caggagagtt tttatatagg
    29401 tctaaaatag tagggggatc tcctatctta tgttcataat cttctgggtc atgtcctcca
    29461 taaaaggtga aggttccttt accgaagttt ccatgtatgt atttaacttc attctgtcca
    29521 ggaacttctg ccagtatgat gacactcttt ttaactttat ctttgaagaa agaagtggtc
    29581 tgacctaaaa agccagggat aatattggtg tgacattgag taagcatagt gggaacaggg
    29641 tcatacttag cggaaaaatc aaagagttga aaataatcag cttccggacc tctcagacga
    29701 gaataatcgc tggcatcaat atcggaaaac tcatattcaa aaggattggt tttcagttta
    29761 aaattatcaa aagcaaaaca acgactataa tccagctttt cctgataatg agggtcaatt
    29821 ccatcaccat caattaaagc atcaactata tccacattat gagcagctaa agcaatatca
    29881 agagtatctg tggcagcaca catagcaaaa agaaaacctc cattcatcac aaactctcgg
    29941 attttttcag ctactgcatg ctttaactgc cagactttgg cataaccatg ttttgctgcc
    30001 attgcttcat tcacccgaac atcattttga taccaggcag tattacgata ggcaccataa
    30061 aactttccat actgaccagt aaaatcttca tgatgtagat gtagccaatc ataatcgctt
    30121 agttttcctg ctaaaacttc atcatcccac acacggtcat aatctatttc tgcataatcc
    30181 agagcaagag tgacagcatc atcccagggt aaagcattag ggggaatgta aacggcaatt
    30241 tttggttcct tttccagaac tactacttcc atattggatt cctgaatctc ctgcataatt
    30301 gaagctacct gggctgaaga taccagctca aaacggacgc cacgaatatt acaaagagca
    30361 gcaagagatt ccgattctgg cataagaaag cttccaccgc ggtaattaag aaaccatttg
    30421 accacatact gctcttttaa ggcttcaaaa gcaactccat aagctttcag atgattactc
    30481 tgagtgaaat ccatgggtat caatatttct gcacctatga gagcaggaag caggattata
    30541 agcagaacaa tgattttctt tactggcata tttttggtct ctttgtttta tgagagctgt
    30601 atcttatttt aaatcttaat attatattat accttaattt agattaaaac ccttatacct
    30661 tgttaatttg tcaagacaat tttagctcgc tttaaatagt aactactttc ttttaaatag
    30721 aatattgcaa ttttatagta aagctatttt tatttcagaa aatcaatatc tttaaatgtt
    30781 tgttttatta tttactcttt gctttcatat cctccatctt tgttaaacct gagcatagtt
    30841 tatatgcaat tgatgagcaa cttatgtcca gttcggacat aagttggaca taagctggac
    30901 ataagttgga catatgctgg acttttagaa cccatatgtc aatgtggcta aaaaaaatag
    30961 aggataatat tatatataac aacaaaatag ccttcctgaa gattattgat gacttcctct
    31021 ctccaagttt gatgttttag cgaactttat atgaaagatt aaaaaagtta agaaaattgt
    31081 tgacaactct tcttagctaa ttttaaaagt attttataat tatgcgctga atattatttc
    31141 atattgtagg ttaaaggtga aaatagttga attactttaa agggagcttt ttaataatat
    31201 acagggaggt aataaaatta taatgatgaa aaggatagtt ttttactacc gttcttgaag
    31261 aaatgtaggg tttataatta tgagttccaa gcaaaaagct ctaaattgtg ctaaatctta
    31321 agagtgatat aattagcatt gaaaaagaat agcattagat gcagtacact attataacct
    31381 tttgctttat acccaaaatg tttattttaa acccatcttc aacattttct actgtcatct
    31441 atcatatttg taaataacaa cctgctattt tagcattatg tctttacaaa atattaatga
    31501 tttcttttca gttaaacttt aaacctcttt caagataaaa aaggagtata ttatgaaaga
    31561 tttaataatt ctcgtaggaa caaatccctt gccgtgttat ataagtgca
//
LOCUS       DAORZF010000014        30925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_1243_length_30925_cov_335.342792, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000014 DAORZF010000000
VERSION     DAORZF010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 30925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30925
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1243_length_30925_cov_335.342792"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            236..3127
                     /gene="uvrA"
                     /locus_tag="PK015_02915"
     CDS             236..3127
                     /gene="uvrA"
                     /locus_tag="PK015_02915"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001503.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOL33458.1"
                     /translation="MKSKIIIKGASEHNLKNINLEIPRDKLVVFTGISGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGQMEKPKVDYIEGLSPAIAIQQKTASKNPRSTVGTV
                     TEIYDYLRVLYARVGKQYCHICGNPVGAQTIDQMTAHLMQNPEKTKIQILAPLVQSRK
                     GEHKEILDDLRQQGFVRVLINGQLRNLEENIVLDKKSKHNIDVVIDRIVIKPGIESRL
                     RDSLELALKLTEGVVKVDFPDEGETLIMSATNSCPHCNIGFEELTPQHFSFNSPIGAC
                     PECNGLGYKLEFDPDLVVPYPDRSIREGAVAPWGSLENKIDTWTGRTIQNLANYYNFS
                     LDTPWYQLPEWVKSLILYGSKGKRFRMEWQNKNGHGVYNMRFEGVINTLDRRMRETTS
                     EDARRYYLQFISDKPCPECGGKKLKASSLAVKVGDKNIAEITAMSVRQAFNFFNNLKL
                     KGNEQIIASEILKELRNRLGFLQSVGLHYLTLDRRSPTLSGGESQRIKLASQIGNQLV
                     GVMYILDEPSIGLHQRDNSMLLQMLCQLRDLGNTVIVVEHDEETIRTADMIVDFGPKA
                     GIYGGEIVATGTVKDIINSNRSLTGDYLAGRKSIPIPKVRIPAEQGYLSIKGAKHNNL
                     KDIDVDIPLGLFVCVTGVSGSGKSSLINQTLYPYLHNYFYHTSHKVGKVDTIEGIENI
                     DKIISIDQQPIGRTPRSNPSTYVKVFDPIRMLFSQLPASKMRGYSAGRFSFNVKGGRC
                     EACEGAGVKQIEMHFMPDIYVTCEACKGKRYNQETLSIRYKGKNIAEVLDMDVQEAYE
                     FFQNIPAIRNKLQTLMEVGLDYIKLGQPSTTLSGGEAQRIKLSRELSKVSTGKTLYLL
                     DEPTTGLHFDDINRLLQVLKKLVKMGNTVIVIEHNMDVIKSADWVIDLGPEGGDEGGY
                     LVAEGTPEQIMKNKNSYTGQFLKQHYGRAKVEKPDISRKPAKKIVKKAI"
     gene            3135..5315
                     /locus_tag="PK015_02920"
     CDS             3135..5315
                     /locus_tag="PK015_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOL33459.1"
                     /translation="MAEIQFRYQPPTSGKHLVGLAGDFTGWEILDMNEVGGVYFLNLH
                     IDEGRYCYKFIVDGNWIPDPDNPLTEPDPFGGFNSVLIVQETIPSRFSWEEIIQDLSL
                     LDERKGYYVDLNRISDEQYELRFNWFPSLPADLIALIDGETFPLYRLGITGNQEVFHC
                     LFTCPRTFCNIKIKIQGADNILFWGAEGFSFQEDGVTPYLLELSNYPIFSVPEWVSHS
                     VIYQIFPDRFCNGDPSNDPDFSEDYYKDCRTPPPPGEYLPAHAEYFHLVEDWYDISYL
                     KQSPWLPEGKPDWWCFYGGDIEGVRQKLPYLKDLGVNLLYFNPLWQAKSNHKYDAADF
                     KKVDPHFATEDEMKAFVKEAHQNGMKIILDVAFNHTGEAFWAFRDVVEKGPNSPYWLW
                     YDWYKWPLPNPLPPDFNPREYYQCWWGIKDMPDLNYDLSRPHPAENYVRDINKAVPNR
                     SLVNHILECVQWWLMNIDIDGFRLDIPNEVPYWFWELFRQEVRAMKPDAWLVGEIWHS
                     GKGWVNHHYFDSVMNYAYFKDPVLEFFILGICDKKNFCARIENGLAQYPIQGIKAMMN
                     LLGSHDTIRILELAKGDVSKVKLALLFQMTFIGAPHIYYGDEIAMLGGKDPDNRRPFN
                     WKWEEDSVAVDLRNYYKNLIQLRLSHPLLMDGEFSFLPAPEGLLAWRRYDENHKIDIV
                     LNYGKENNHLESISASKIIFTEGKVDHNDNSLMLYPNSAIVYIS"
     gene            5441..6616
                     /gene="coaBC"
                     /locus_tag="PK015_02925"
     CDS             5441..6616
                     /gene="coaBC"
                     /locus_tag="PK015_02925"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOL33460.1"
                     /translation="MEGKILLCVTGGIAAYKAIDLASQLTKSGFEVKTAMTKNALKFV
                     SALNFSAITNQSVHTDLFEDSDPIPHIHLADWADLTVVAPATANIIAKAAQGIGDDLV
                     STILLSQPKPVLYVPAMNVHIYEHPATQNNLKILKDRGNYILEPETGLLACGYEGKGK
                     YPPNEEVIYAIRCYLKYPTDLTGKKVLVTAGATAEPIDPMRMITNKSSGKMGLSIARA
                     FALRGAEVTLIYGIMSESIPYYIKKSIFAPTAKLMYDAVMEHSKDYDIIVQCAAVSDY
                     KPVKPSTQKLKKSGNFNLELVATPDILEHLGKIKPKKQKLVGFAAESEHILENAREKL
                     EKKNLDLICANNLETAGKDETTLNLIRAGTTKTTKSVQLTGNKFDVALQLVEYIVSL"
     gene            6613..7395
                     /locus_tag="PK015_02930"
     CDS             6613..7395
                     /locus_tag="PK015_02930"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOL33461.1"
                     /translation="MKNKLTILTGANGQVGSFIAHQLAEKNAPLLLLYHKNFNRIEDL
                     ADKEKVMLISCNLEKLEDVNPAIHYAAENLAAVPAFLIHTAAIRSYDALPLAQTNPEI
                     FKEVLSSNLEMAYNVLRTCLPFMLQERFGRIVMFGSNVVQTGLYRGSAYAAAKAGIVN
                     LVKSIALETASSNVLINAISPAPVQTVLEEEYTGDYLAFRKRYFEEFRQMSPTGKLVS
                     KEEIWLFCNLLLSEKLENFTGKDIIIDGGFSAIQQIKGNEIQ"
     gene            7392..8165
                     /locus_tag="PK015_02935"
     CDS             7392..8165
                     /locus_tag="PK015_02935"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOL33462.1"
                     /translation="MKVKLRFYLLLIICLFSTSLFALPFQDGEKLTFSVKYGIIKAGT
                     ATFEAKSSTYQNRPVWRLCITAQTYPFFDVVYKIRDKIESWWDKETLLPHKSSKQLHE
                     GKYQQYRIHYFDQVNKKTTYMKWSYKEEKWKTEEKNLPFETQDIVSSFYELRNRSLKP
                     GDKPKLYVTTDGKSVVTTVKVLRREKVSSIFGDVNCLVLEPDMKSEGIFKGSSKLLIW
                     VTDDAYKIPVKVESSVSIGTFVVQLEDAQNVPYKIKKSN"
     gene            8165..9199
                     /gene="queA"
                     /locus_tag="PK015_02940"
     CDS             8165..9199
                     /gene="queA"
                     /locus_tag="PK015_02940"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOL33463.1"
                     /translation="MVDLLDLNSYDYYLPQELIAQYPLKDRSSSRLMHIDRKTGTISH
                     LYFRDLVDLLAPGEVLVINNSKVIPARLYGYKDNGTKIEILLLNHLEGSKWKCMVHPG
                     KRLKREQVLRFSPQLEGWVSLPDEDGLRVIEFNNPDTYWNEIERIGHIPLPPYIKRED
                     KAEDRQTYQTVYAEEAGSVAAPTAGFHFTEEMLSQLKEKGIKIVNIILHIGMGTFVPI
                     KTQRIDQHKMHSEFCTIPEETANVINKAKLEGRRVIAVGSTSIRTLESFWNNGTLMSG
                     SKWTDIFIYPGKNIQVADALITNFHLPKSTLLMMVSAFAGYELTRKAYEIAVQEHYRF
                     FSYGDAMYIS"
     gene            9196..9912
                     /locus_tag="PK015_02945"
     CDS             9196..9912
                     /locus_tag="PK015_02945"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOL33464.1"
                     /translation="MKTKLLYTLLDSTMNEYQRLTQFCTGTHQWIIRAREQKEGRGRE
                     DRFWYSPEGGLWMTFDIYYDDFIPTFPLFIAYCLHSLLLELFPLESLSIKWPNDIMWK
                     DKKLGGILCEHNESEKKYIIGLGLNTNVSQDAFDGKLDIVTLSSLIGFPVSNEYLSDL
                     IIANIHKKIDLLTNPKFYLNYINLWLYGLNQLAQVQLDNKIVTGINIGVAEDGTLLLK
                     DREGYIHEINYGSLNYIPDL"
     gene            9985..11595
                     /locus_tag="PK015_02950"
     CDS             9985..11595
                     /locus_tag="PK015_02950"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOL33465.1"
                     /translation="MKKWMIIVLAVSLVLISCASNKKEVQPEQVQKAEITTKVAILPL
                     KALDSSSRYITKILTVRDLELTFNKYPNYTLLDMNETAAVYNESGNLDVEDIEKEELD
                     NLAKQLGADVIIIATVSETRTDIFNISMRMYSTVSTDLKQISFNVGKEKTARWKALEE
                     YMMKELDDFVSNEIDKLFNIAANHFNNGNYTTAEQILKQVIALKPEKVDAYYFLGNTY
                     IKLEKIELAEQNFQKALEIDPQHQASLIALIDLYDKTNQPMKRIPLMERIAEETRDAE
                     TWFAVGNLYNQIGNKEKAKESYRKALAIDPEFTGAIVNLSIILFDEGSYNEAIPYLEK
                     AFEKAPDNTFIAARLATAYQRSGRTQEAIDKYENVLAKDPNNIQAYLNLISIYRSNGN
                     NTKAIEKINALKKVAPDNPYSYLNLAAIYLEQGKLNDAETNANITISKDPTLYQPYII
                     LSSVYQSRGAESYNKYIDLDRQASRAVGKKALDLKKQRDTAKTTAINHYNKALNYLQT
                     ARNYTDDPEVLSDINSRIAYINQLLAQF"
     gene            complement(11685..12833)
                     /locus_tag="PK015_02955"
     CDS             complement(11685..12833)
                     /locus_tag="PK015_02955"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOL33466.1"
                     /translation="MDNIYDVIIIGAGSIGVPTALNLALQKQKVLVIDGESAPGQQNN
                     KKAIGGVRATHSDYGKINVCKRSIEIMKTWKETWGDDIGWMSNGYSYPAYTEEDEKTL
                     KDLMKVQHSYGLNITWVSPEEYNELVPGINKEGLRGSTYSPEDGSCSPLLLGSAYYFH
                     SLEAKVQYRFREKVIGFDMSQSKIEKVITDKGTYSTGMVINAAGNYGKEIAAMAGLNL
                     PVQPENHEAGITEPVARFMGPMVVDMRIRPGSENFYFYQNNEGQIVFCITPKPPITGI
                     DNRSTSVFLPMCTKRMLEVYPRLHSLRVRRTWRGQYPMTPDGFPIVGKSGDNLINAIG
                     MCGQGFMLGPGLGELITRICLDELTESDLHILQSFNPERDFSGMEAFK"
     gene            complement(12836..>13282)
                     /locus_tag="PK015_02960"
     CDS             complement(12836..>13282)
                     /locus_tag="PK015_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOL33467.1"
                     /translation="MDIEGNSLGFYTIISVIDVKKRHTQLIKVQVPKAIARKIASFRI
                     QAKEVSEPLPEPVIPDKMPDEAMICLCERVKAGDIRKLIHSGVTNLNQIKAITRAGMG
                     PCGAKTCENLILQLLREEGIPIEKVVPNTKRPLFIEVPLEKFPDGD"
     assembly_gap    13283..13382
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13383..14743)
                     /locus_tag="PK015_02965"
     CDS             complement(<13383..14743)
                     /locus_tag="PK015_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOL33468.1"
                     /translation="MENHRILKHPILPIEEREDVLFSWNGEPLLAKKGEMISSALIAN
                     GINVFGHHHKDGSAQGIFCANGQCAKCTVIADGVAVKSCMTAVTSGMEVRSLEGLPEL
                     PETPFIPKFPPLEKLEVEVLIIGGGPAGLSAAIELGKYNIDTLLIDDKQALGGKLVLQ
                     THKFFGSEEDSNAGIRGIDIGKLLAQELQQYSSVKVWLNSTALFVFSDKKVGILKDGI
                     YKLVTPHIILNAAGAREKFLRFPGNNLARIYGAGAFQTLVNRDLVRPTNRLFIIGGGN
                     VGLIAGYHALQAGINVVGLAEAMPQCGGYKVHSDKLKRLGVPIYTSHTIINAEGKETV
                     ERITIAEVDSKFHPIPGTEKSFACDTLLIAVGLNSLSEFTEEAKEAGIQVFAAGDAQE
                     IAEASSAMFNGKIAGLTIAKECGKDEVEAIPSEWYAKAEVLKSHPGAIKDYQKDLPET
                     GVFP"
     gene            complement(15290..>17219)
                     /locus_tag="PK015_02970"
     CDS             complement(15290..>17219)
                     /locus_tag="PK015_02970"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOL33469.1"
                     /translation="DCRNTDSVPQENFDIYGQHLSTSGQPLWEPNGRIICNDPYKQVN
                     PILIFNIDMIYCAWSDERNSSPGIYYQVYNSNDIAQLVENGKMIFWGIGGGIDRPLIA
                     NRSQDVVVMWCDSRDYETLGYKIFFQFLNSDGSLKLEPNGRPITLPYSGNQLSPSVVV
                     LPDDSIIFAWVDDRNGNDKIYLQQIDSNGNRLWGDYGIQLTESNPFYQYNPHISYLPD
                     LDQVYIGWSSFDYQNGNPTPFSVYGQMLYNGEKQWGPNGILISVNDGADENSTEFYIN
                     DVKGNYFIWTTVDYTQFYQKIFVKKVAPNGEASPGWEEYGLSVSMSNDIYTYQYYPLV
                     TLTSEGIFVLWTKSVYNSNNLFYFAQHISSDGERLWGPEGMEVLTNAYINNYSVVENP
                     LFQNEIVVVWDNYINNLDIKAQKFSLQGLPLWNENGIYIIQNPENQFEPIIGRFDNGG
                     MIVAWTESPQYSTYYDNNLYYNYLNNDGTVGGSVSEGYVLCNIFNNQNSPKLTIHNNE
                     AYIIWSDYRSSNEAFEDGYNSLYAQKISNETPINDPSTPFISNFQLLQNYPNPFNPST
                     NIKFLVNDITLPYTLNIYNIKGQLIKTLFKGNLEKGTHNIIWDGTDSNDKPVSSGIYL
                     YSLSNGKDNQIKGMVLMK"
     assembly_gap    17220..17319
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17320..18122)
                     /locus_tag="PK015_02975"
     CDS             complement(<17320..18122)
                     /locus_tag="PK015_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33470.1"
                     /translation="MKKFIFIGIMLCLLFSLKAATLWENEVPIRLGANIEWYSSGAKT
                     SDGGVIYVWSDTRRGDRDIWAQKVDAQGNIIWNEPALIDGKLNHQDEPVIIQTSDDNF
                     VIAWIDYLNDPYGTDIYAQKINSQGQLLWQTGGIPVCIANENQTYIKIVSDNSGGVYI
                     LWNDYRNYYWKLYGQHLDSQGNPIWDINGIQLDNDNSFNYYYNALSDGQDGLIVAYPN
                     FDGTNQNIYINRYLYDGSMAWNEPVVLDNSPGYHLNIEASLLSNGEIMF"
     gene            complement(18229..21297)
                     /locus_tag="PK015_02980"
     CDS             complement(18229..21297)
                     /locus_tag="PK015_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOL33471.1"
                     /translation="MKKSIFFLLLMGLIINLSAELLWQEPIPIRQGVNIEWFRSGTQT
                     ADGCAIYVWSDTKLGERDLWAQKVDAQGNAVWGEPLLIDGKPDRQEDPVITMTSDNNF
                     VIAWIDFSDDLDGNVYAQKINAQGQLLWQTGGVPVCTYPNVQISLNMESDNEGGVYII
                     WVDSRNPGKDLYGQHLNSSGAPLWQVNGIPIANDIGDEVQNTMLPDGQGGFIIAYTYS
                     FGGLEDIFAKRFLSDGTMAWQQPTIISNAPGNQGKVRMATLTNGEFVFVWEDQRNEDP
                     DIYAQKIDINGNILWRDPFIVFGDSGTANFAPQLNPRIVATSDNAVIIVWEDKRLDNY
                     ATDLFVQKINAAGDLLWDVDGVPLCVAEFAQNEARLTPDSNGGCYIVWDDLRNGDTPN
                     DDIYGQHLSATGTALWEANGRPICNAPNQQNSALVKVSNNMVFINWMDMRNGSVGLYY
                     QVYNSAGVAQLPENGELVFWGLSGDTPLNNYLILPRSQDVVIIWQDTRFANMGYQIFF
                     QFLNSDGSIDLEPNGRPVTLSTGANQSNPAAAVLSDDSIVLAWVDARDENPNIYLQHL
                     DANGNRLWGDYGIPLTESIPIEQKNPRVSYKADQNQVYISWSNYESHSGNLRFYVYGQ
                     MIQNEQKQWGPDGIKISYYPEEEFLYHECSQNELKENYFTWQDANLIEFTQSIFTKKV
                     DANGQTAQGWPESGLQVSTYPITNWDCRQRFPVSTLTDEGIFVMWKDARGDDIENYYG
                     QHISSNGERLWDPLGVNLADYGREQLNVSIAENPLYRNEIVFAWTENIAGMGDILTQK
                     YSLTGIPQWGDLGNYVVQKDSMQTSPYLARFDNGGMVIAWTDYYNTITEESNINYKYI
                     KSDGTFVCDNPGGYVLCDAKKNQYNPMIAIVGNEAFAIWADGRSSGKTEILGLYAQKL
                     SNEIVALDDPTTPSISQFKLIQNYPNPFNPSTNIKFTINDITNPYTLNIYNLKGQLVK
                     TLVKGNLEKGTHNIIWDGTDSKGETVSSGIYLYSLSNGKSNQIKRMVLIK"
     gene            complement(21398..22618)
                     /locus_tag="PK015_02985"
     CDS             complement(21398..22618)
                     /locus_tag="PK015_02985"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOL33472.1"
                     /translation="MRKIRTNIVNPVSPDYTFIGLDYVISWEDGIINNIHPYQSGVDL
                     DCEERLDELCLPGLIDIHTHLSQYRIRGRYEPSLLSWLEKNVFPEEALSQNPDFAEKI
                     ATDFFQALFAAGTTTSVIYTAPYPQACETAFSIAQKFGARTLIGMTLMDQNAPSELLQ
                     STDFVFQKSVELYHKYHNVSPLQDYIFSPRFAISCSFNLIEKIARFAQDNSAWIQTHL
                     SENQIEIANVIQLFGTDTYTQVYENAGLLTPHTILAHCIHLSPAEMDILAKYDCKIAH
                     CPDSNFFLKSGEFNYEALYSRNLKIGVGSDVAAGTTLNMLYHSKMANYRQSVYSISPE
                     RLFYHITLGNATMLGYQDKIGSLDSGKEADLCFLKLPPEPIPEDSLISAICFYGYEFP
                     VMETVIAGRTVYSR"
     gene            complement(22599..25283)
                     /locus_tag="PK015_02990"
     CDS             complement(22599..25283)
                     /locus_tag="PK015_02990"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HOL33473.1"
                     /translation="MKRFLIICFLSFSLLFAITQDDFRWLMDTEQFSLIEKYSLSWQK
                     WQEGGKEELSLLLEYSQRAEKPELELKCNEILALKYHQLENALRWINTAERLQLNNQQ
                     IEEMYLKLSQVFTNPQDRITLDYYFGQITQEEFLEQILQLRGYNQIIEDIARGWIDEI
                     SSEYSDSLALAMINRFEYHFPLSQWTQATYFYKLYHYLSLGQYDIMHQCIQEQGYRSA
                     ECLYISALYTLSPDYRRNQELANNRLILNQAIYMLDSALKDYSSDQEIRVVYDLYTPE
                     QWRNRLLLTKVKGEYYSLLADLNLYGDEENLYALFNEPNDALLQLLEDISVIQFDNND
                     RGELAELHYWQGKINALINQKKYLQTAAKEYTTALVYGSPRKKYDDSCLHDLEFLRNK
                     LNVDKELYAWLHEIMNYDGIIFEEYPFPDKHYSRIAIGDYDNDGYNDLLFDGHSLWHN
                     DSGQGFTEVSDTMNVANLRANGALWADFNRDGYLDFVTISHNSEGLGESLMKNQEGLR
                     FVKVNERAGDVDDHYPTEGAAWIDIDGEGYPSLYCANYEQWQIRSGYPDFFWYNDHGY
                     FKDESVKRGFRSPTYADNPGLAGRGVAPSDFDNDGKQEILVTNYRLNRNFCWDQKDSI
                     FVDVAALYGLTGHYQDGYYGHSIGADWGDFDNDGDLDLFVANLAHPRFIEFSDISQLL
                     RNDGLSYKVIESDTLWFWQFTDITKSAGITYDECHSDPLWLDADNDGYLDLFITSIYK
                     NERSYLYKNNGDGTFTDITFLAGARVYNGWGNATADLDRNGLTDLVIASGSGDKILLN
                     KTQTTNNSIFIKPVWQDDKIILIDNPVKFSQFPASPAFGTRVKLILRDADGKQRTLIR
                     ELASAKGTTSQNAQELHFGLGNATVLSIERVKYAKDKN"
     gene            complement(25280..26161)
                     /locus_tag="PK015_02995"
     CDS             complement(25280..26161)
                     /locus_tag="PK015_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HOL33474.1"
                     /translation="MKESDYLWRGMALNGQFRVLAVSSTNTVQAARDLHDLSPINTIL
                     MGKMIPAVAMLSLDLKDEGAEVTLRFDGDGPLKGALTICNNQGDVRGYAFEPKFWLDD
                     LQENFHPVKYLGNGTLTVIRYYLGDRPTKGLTPLIEGDVAQNLAYYFEQSEQIPTAVN
                     LGVLIDKDAKVRASGGFIIQQLPSADRLNADRLIDNINHMPNLSDLMDMGMYLPEILH
                     SFVFKEGGLELYPVHRVRYRCHCSKERFAAALKLLSLDELKELRDGIDPVCQFCNATW
                     HFSAAEIEEIISEVEKK"
     gene            complement(26148..27617)
                     /locus_tag="PK015_03000"
     CDS             complement(26148..27617)
                     /locus_tag="PK015_03000"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOL33475.1"
                     /translation="MSLNPILRAAEIRNGQLYLEVLIEVDNKITLKPEKESESTINIY
                     ESDLPVCRKMNGKLEIELIKTDKQACWGILTRTEPWTDDSEGIPYESFVWLENHLRLK
                     RENLIYADTSFVSRDSALAYARAQGIPDKMVQYIPIVGSTVKITTARKEKYYLETPLR
                     IHSDFPIIVNSQKEAYSGEFYLKEIDNRLVFTQLLYLEEYIAGVIPYEIGGNAPIEAM
                     KAQAITARTHSIALLTFNRHKNDGYDLCNSTHCQVYKGRFINNPNVQIAVDSTSNQIL
                     TVNGRIADTPYHSNCGGHTDAAHQIWISQPLPHLLGVPCVAGVDSLDLTQENQARQWI
                     DTDTLDPMMNSWEKAGMCWSHTISRKKLASNAGMDDIDKIVINQRSISGRIIDMSFYN
                     SGETRILGEAKIREIFGGLRSSFFYIDGKYNFNRNGKVEIIPGGNLTLKGKGYGHGVG
                     MCQSGALRQAREGKTYWDILNFYYPGTQISTDWMYYEGK"
     gene            complement(27680..29695)
                     /gene="metG"
                     /locus_tag="PK015_03005"
     CDS             complement(27680..29695)
                     /gene="metG"
                     /locus_tag="PK015_03005"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOL33476.1"
                     /translation="MKYLVTSALPYANGRLHIGHIAGAYLPADIFVRWLRLHKEDVVY
                     ICGTDEHGTPISIAADQEGVSPQEIVQRYHESIKNAFEALEIEFDNFSGTARPGHYKL
                     SQEFFLTLYKNGHILPRKTKQFYCETDHRFLPDRYVEGICPRCHATGARGDQCDACGQ
                     IYETTSLIEPRCKICGNTPVIKESTHWYLQLNDFKDQLADWISKKDYWKENVRNFMLN
                     LLEQGLVERPITRDLNWGVPVPLEGAEGKVLYVWFEAPIGYISSTQEWAEKIGEPNRW
                     KDYWLDPETRLIHFIGKDNIIFHALIWPAMLMGQDKAYCLPYDIPANEFMNLEGQKIS
                     TSRNWAIWVDEFVEKFPAEYLRYYLACIAPEKNDSDFSFYEFQQKINRELNDVLGNLA
                     NRVFTLINKHFNGLLEPIEFSDSSLKTLEEAQELYKQIDEGYKNYQVKWNTKQAMDIA
                     RLGNRYFDERKPWSEIKKDSNSVQETLYVCLTLLRMVSVALYPVMPESMNKLRKMMGL
                     PELSVWEEIDLKSNYNLNKIEPLFFKLEDSVIEEEVKKLKQNAKLNSSKINQIPIKEF
                     ISYDDFAKIDIRVATILSAEKVPKTDKLLHLKVDIGNEERELVAGIAEHYKPEDLPGK
                     QALMLVNLEPRTIRGIRSQGMILAIESEGNLTLAVPEKYSPPGSVVK"
     gene            complement(29692..30870)
                     /locus_tag="PK015_03010"
     CDS             complement(29692..30870)
                     /locus_tag="PK015_03010"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOL33477.1"
                     /translation="MAIIVKKFGGTSLGSIELIRNIAHRIALEAGKGDSLVLVVSAMA
                     KTTDDLINLAYQISEHPSRRELDMLLTAGERISMSLLSLALQEEGYSSISFTGSQSGI
                     ITDERHGNARILNVNAFRIKEELDKGKIVIVAGFQGVSQSKEITTLGRGGSDTSAVAL
                     ACYLQAKKCEIYTDVDGVYTADPKIVSNTKLLPAIDYSQMLALSYNGSKVIHPRAVEF
                     AYKYSIPVEIKSSFTFAPGTMLIQEESFEEEKNMEERHITAIAHKENLLSYIIPETAE
                     TIDLLKIWNWEIFKYLAIENQLELIIEEKYDKEIRYLLSQKEIPILKTENGIGYVTLV
                     GLGINLDPSFLADTLKLCQPFHIKRIQHSDKTIEIMLPSSEVKDCVQILHSAFIGCEK
                     "
BASE COUNT         9888 a         5676 c         5393 g         9768 t
ORIGIN      
        1 gctaattaat gtttgaaagt gagacaaaga atggattttt aaaattaata aggcttattt
       61 cttataaatt caataataag cctattatat ttcaagtaaa atgtaataag attaatgata
      121 aatatttaat agtcagtata ttatattaat ataaacttgc ctgagaaaga gtcaaatgat
      181 tttatttgac aaatcaaagc ctaagaaaat tgctgaaaga tagcaaggag taattttgaa
      241 gagcaaaata attataaaag gtgccagcga gcacaattta aaaaatataa atttagaaat
      301 tccgagagat aaacttgttg tatttacagg tatctcaggt tcaggaaagt catctttggc
      361 ttttgatacc ctttatgctg aaggacaacg cagatatgtg gaatctttat ctgcttatgc
      421 ccgacagttt ttgggtcaaa tggagaagcc taaagttgat tacattgaag gtttatcacc
      481 agctattgcc attcagcaaa aaacagccag caagaatcct cgttccactg tgggaaccgt
      541 taccgagata tatgattatc taagggtttt atatgcccgg gtaggtaaac aatattgtca
      601 tatttgtggt aatcctgttg gagctcaaac tattgatcag atgacagctc atttaatgca
      661 gaatccagag aaaactaaaa tacagatact tgccccctta gtgcaatccc gtaaaggaga
      721 acataaagag atattagatg atttaagaca acagggattc gttcgtgttt taatcaatgg
      781 tcaattgcgt aacctggaag aaaatatcgt tttggataaa aaaagtaaac ataatataga
      841 tgtagttata gatagaatag taataaaacc cggtattgaa tcaagattac gagattctct
      901 ggaacttgct ttaaaattaa ctgaaggtgt ggttaaagtt gattttcctg atgaaggtga
      961 aactctcata atgtctgcta ccaattcctg tccgcattgt aatatcggat ttgaagaatt
     1021 aacaccgcaa catttttcct tcaatagtcc gattggtgct tgtcctgaat gtaatggact
     1081 gggttataaa cttgaatttg accctgattt agtagttcct tatccggata ggagtattcg
     1141 tgaaggtgct gttgctcctt ggggaagtct ggaaaataaa atagatacct ggacgggaag
     1201 aactatacag aatctggcta attattataa tttttctctg gatacaccat ggtatcagct
     1261 tccggaatgg gtgaaatctc ttattctcta tggttcaaag ggtaaaagat tcaggatgga
     1321 atggcaaaac aaaaatggac atggtgtcta taatatgcga tttgaaggag ttattaatac
     1381 cctggataga agaatgagag agaccacatc tgaagatgcc agaagatatt atttgcaatt
     1441 tataagcgat aaaccctgtc cagaatgcgg tggtaaaaag ctgaaagcaa gttctttagc
     1501 tgtaaaagta ggtgataaaa atattgcgga aatcacagct atgagtgtgc gacaagcttt
     1561 taattttttc aataatctaa aattaaaggg taatgaacaa atcatagctt cagaaatcct
     1621 aaaagagttg cgaaatcgtc tcggattctt acaaagtgtg ggtttgcatt atctcacctt
     1681 ggatagaagg tctccaaccc tttctggagg tgaaagtcaa cgaattaaac ttgccagtca
     1741 aataggcaat cagcttgtag gtgtgatgta tattctggat gagccaagta taggtctcca
     1801 tcaaagagat aattctatgc ttttgcagat gctctgtcaa ttacgagatt tgggtaatac
     1861 cgtcatcgta gtagaacatg atgaagaaac cattcgcacg gctgatatga ttgttgattt
     1921 tggtcctaaa gctggtattt atggtggaga aattgtagcc acgggaacgg ttaaagatat
     1981 cataaatagt aatcgttcct taactggaga ttatctggca ggaagaaaga gcattcccat
     2041 tcccaaagta aggattcctg ctgaacaggg atacctttct attaaaggtg caaaacataa
     2101 caaccttaaa gatatagatg tggatattcc tcttggatta tttgtttgcg taacgggtgt
     2161 ttctggaagc gggaaaagtt cattaatcaa tcaaactctt tatccttatt tgcataacta
     2221 cttttaccac accagtcata aagttggaaa ggtagatact attgaaggaa tagaaaatat
     2281 agataagatt atcagtattg accagcaacc tataggaaga actcctcgtt ccaatccgag
     2341 cacctatgtt aaggtatttg atcctattcg gatgctattt agtcagttac cagcatctaa
     2401 aatgcgaggt tacagtgcag gacgcttttc ttttaatgtt aaaggtggaa gatgtgaagc
     2461 ttgtgaaggt gcaggtgtta aacagataga aatgcatttt atgcccgaca tctatgtaac
     2521 ttgtgaagcc tgtaaaggta aaaggtataa tcaagaaact ctatctattc gctataaagg
     2581 gaagaatatc gctgaagttc tggatatgga tgttcaggaa gcttatgaat ttttccagaa
     2641 tattccagct attcggaata agcttcaaac tttgatggaa gtaggtctgg attatattaa
     2701 attaggacaa ccttcaacca ccctttctgg aggagaagct caaagaataa agctttcccg
     2761 tgaattgagt aaagtgagca cagggaaaac tttgtattta ctggatgaac ctacgacagg
     2821 tcttcatttt gatgatataa accgtcttct gcaagtatta aaaaaactgg ttaaaatggg
     2881 aaatacggta atagttattg aacataatat ggatgtgata aaaagtgctg attgggtgat
     2941 tgatttaggt ccagaaggag gagatgaagg tggttatctt gtagctgaag gtactccaga
     3001 acagataatg aagaataaga attcatatac cgggcaattt ttaaaacagc attacggaag
     3061 agcaaaagtt gaaaaaccag atataagccg aaaaccggca aaaaagatag taaagaaggc
     3121 aatttaatca ttttatggca gaaattcaat ttcggtatca gccaccaact tcaggaaaac
     3181 atttagtagg tttagcagga gattttaccg gatgggaaat cctggatatg aatgaagtag
     3241 gtggagtcta tttcttaaat ttacatattg atgagggaag atattgctat aaatttatag
     3301 ttgatggaaa ctggattccc gatccagata atcctttaac tgaaccagac ccctttgggg
     3361 gtttcaattc tgtcctcatt gttcaagaga ccattccttc tcgtttcagc tgggaagaga
     3421 taatacaaga tttaagcttg ctggatgagc gaaagggata ttatgttgac cttaatcgca
     3481 tatcagatga gcaatatgaa ttacgattta actggtttcc ctctttacct gctgatttga
     3541 tagctctcat agatggggaa acttttccct tgtacaggtt aggaatcaca ggaaatcaag
     3601 aggttttcca ttgtcttttc acctgtccac gaaccttctg taatataaaa ataaagatac
     3661 agggagcaga taatattctt ttctggggtg ctgaaggatt ttcttttcag gaagatggag
     3721 ttactcctta tttattagag ctctcaaatt atcctatctt ttctgtgcca gaatgggtat
     3781 cacatagcgt tatctatcaa attttcccag accgtttctg caatggagac ccctccaatg
     3841 atccagattt tagtgaggat tattataaag attgtagaac acctcctccc ccaggggaat
     3901 atttaccagc tcatgcggaa tattttcacc tggtggaaga ttggtatgat attagctatt
     3961 taaaacagag tccctggcta cctgaaggga aacctgattg gtggtgtttt tatggaggag
     4021 atattgaagg agttcgtcaa aaattgcctt atctaaaaga cctgggggtt aatctgcttt
     4081 attttaatcc gctgtggcaa gccaaatcca atcataaata tgatgcagca gatttcaaga
     4141 aagttgatcc ccattttgct acggaagatg aaatgaaagc tttcgttaag gaagcccatc
     4201 agaatggtat gaaaatcatc ttagatgtag ctttcaatca tacaggagaa gctttctggg
     4261 cttttcgtga tgtggtagaa aagggtccaa attctcctta ctggctctgg tatgactggt
     4321 ataaatggcc tcttcctaat ccgcttcctc cggattttaa tcctcgggaa tattatcaat
     4381 gctggtgggg aataaaagat atgcccgact taaattatga cctcagtcgt cctcatcctg
     4441 cggaaaacta tgtgcgtgat atcaataaag ccgttcccaa tcgttctttg gtaaatcata
     4501 ttctggaatg tgtccagtgg tggctgatga atattgatat agatggtttt agactggata
     4561 ttcctaatga agtcccttac tggttctggg aactttttag acaggaagta agagcaatga
     4621 aacctgatgc ttggcttgta ggagaaatct ggcatagcgg taaaggatgg gtaaatcatc
     4681 attactttga ttctgtgatg aactatgcct atttcaaaga tcctgtgctg gaattcttta
     4741 ttcttggaat ctgcgataaa aagaatttct gcgcaagaat tgaaaatggc ttggcacaat
     4801 atcctattca aggaataaaa gcaatgatga atctactggg aagtcatgat accatcagaa
     4861 ttctggaact ggcaaaaggc gatgtctcca aagttaaatt agcattactt ttccagatga
     4921 cctttattgg agcaccacat atctattatg gtgatgaaat tgctatgctt ggtggaaaag
     4981 acccagataa tcgtcgtcct ttcaactgga aatgggaaga agattctgta gctgttgacc
     5041 tgagaaacta ttataaaaat cttattcaac ttagattatc ccatcctctt ttaatggatg
     5101 gagaatttag ctttcttcct gctccagaag gattgcttgc ctggagaaga tatgatgaaa
     5161 accataaaat tgatatagta ttaaattatg gaaaggaaaa taaccacttg gaatctatat
     5221 ccgcttcaaa aattattttc actgaaggga aagtggatca taacgataat tctttaatgc
     5281 tttatcctaa ttctgctata gtatatatat cataagaaaa tcaatatcta aaatgatatt
     5341 ttatgaagga aactgtataa tagtttgtaa aacaattaaa taatgagatt tgatttgaca
     5401 ttaaagttca aggaaagata aaggatagat aggtgaataa atggaaggaa aaattctact
     5461 gtgtgtgact ggtggaatag cagcttataa agctattgac ctggcaagtc aattgacaaa
     5521 atcaggattt gaagttaaaa ctgcaatgac taaaaatgct ctgaaatttg tgtctgcttt
     5581 aaacttttct gccataacca atcaaagtgt tcatactgac ttgtttgaag attctgaccc
     5641 cattcctcat atacatctgg ctgattgggc agatttaact gtggtagcac cagccacagc
     5701 taatattatt gctaaagctg ctcaaggtat aggggatgat ttagtttcta ccatactatt
     5761 atctcaacca aaacctgtcc tttatgttcc tgctatgaat gtccatatct atgaacatcc
     5821 tgctacacaa aataatctta aaatacttaa ggatagaggc aattatatat tagaacctga
     5881 aactggttta cttgcttgtg gctatgaagg taagggtaaa tatcccccta atgaggaagt
     5941 tatttatgct attcgttgct atttaaaata tcctaccgat ttaactggaa aaaaagtctt
     6001 agttactgca ggagcaacag ctgaaccaat agaccctatg cgaatgatta cgaataaatc
     6061 cagcggaaaa atgggacttt ctatagctcg tgcttttgca cttagagggg cagaagttac
     6121 tttgatatat ggtattatgt ccgaatctat tccttactat atcaagaaaa gtatctttgc
     6181 tccaacggca aaattaatgt atgatgcggt gatggaacat tctaaagact atgatatcat
     6241 agttcaatgt gcagcagttt cagattataa accagtcaaa ccctccactc agaagcttaa
     6301 gaaaagtggt aacttcaatc tggaattggt tgctactcca gatattctgg aacatctggg
     6361 gaaaattaag cctaaaaaac agaagttagt tggttttgcc gcagaatcgg agcacattct
     6421 ggaaaatgca cgagaaaagc ttgagaaaaa gaatcttgac ctcatctgtg ctaataatct
     6481 ggaaactgct ggaaaagatg aaactaccct taacttaatt cgtgctggta caacaaaaac
     6541 tactaaatct gtacaactga cagggaataa gtttgatgtt gcactacagt tagtagaata
     6601 tatcgtaagc ttatgaaaaa taaattaact atactaacag gagctaacgg acaagtagga
     6661 agttttattg cgcatcaatt agccgaaaag aatgcacctt tattattact ttatcataag
     6721 aatttcaatc ggatagagga tttagctgat aaagaaaaag tgatgcttat atcctgcaat
     6781 ctggaaaagt tagaggatgt taaccctgct attcattatg ccgcagaaaa cttagctgcg
     6841 gtacctgctt ttctcattca tacggcagca attcgtagtt atgatgcttt gcctctggct
     6901 caaactaatc cggaaatatt taaagaggtg ctcagttcca acttagaaat ggcatataat
     6961 gtgctcagga cctgtcttcc ttttatgtta caagagagat ttggcagaat tgtaatgttt
     7021 ggttctaatg tagttcagac aggactttat cgtggttctg cttatgctgc tgccaaggct
     7081 ggtatagtca atttagtgaa atctattgct ctggaaacgg catcttctaa tgttcttatc
     7141 aatgcaatat cacctgcacc ggttcaaacg gttttagaag aggaatatac aggggattat
     7201 cttgctttcc gtaagcgcta ttttgaagag tttcgtcaga tgagtccgac gggaaaactg
     7261 gtctcaaaag aagaaatatg gctattctgc aacttattat taagtgagaa actggagaat
     7321 tttacaggca aagatattat catagacggc ggtttttcag ctattcagca aataaagggg
     7381 aatgaaattc agtgaaagtc aaacttcgct tttatctact acttataata tgcctttttt
     7441 ccacatcctt gtttgctttg ccttttcagg atggggaaaa gcttactttc agcgttaaat
     7501 atggaataat taaagctgga acagctactt ttgaagctaa atcttcaact taccaaaata
     7561 gaccggtctg gcgtttatgt attactgctc agacttatcc tttttttgat gtagtttaca
     7621 aaatccgaga caaaattgaa tcttggtggg ataaagaaac tttattgccc cataaatcat
     7681 ccaagcaatt acatgaaggg aagtaccagc aataccgaat acattacttt gaccaggtaa
     7741 ataaaaaaac cacatatatg aaatggagtt acaaagaaga aaaatggaaa actgaagaga
     7801 aaaatcttcc ctttgaaact caggatatag taagttcttt ttatgaattg aggaatcgtt
     7861 cgttgaagcc aggggataaa ccaaaactct atgttacaac tgatggaaag tctgtagtta
     7921 caaccgtgaa agttttgaga agagaaaaag tttcttctat atttggtgat gtaaattgtt
     7981 tggttttaga accagatatg aaatcagaag gtatctttaa gggttcaagt aagcttttaa
     8041 tctgggtaac tgatgatgct tataagattc ccgttaaggt tgaaagctca gtctctattg
     8101 gcactttcgt agttcagtta gaagatgctc agaatgtccc ttataaaata aagaaatcca
     8161 attgatggtt gaccttttag atttaaatag ttatgattat tatttacccc aggaattgat
     8221 agctcaatat cctttaaaag accgtagttc ctcaagattg atgcatattg ataggaagac
     8281 aggcacaatc tctcatcttt atttccggga tttagtggat ttattagctc caggtgaagt
     8341 gttagttata aataatagca aagttattcc tgctcgttta tatggttata aagataatgg
     8401 tacaaagatt gagattttgc ttttgaatca tctggaaggt tcaaaatgga aatgtatggt
     8461 tcatccaggt aaacgattga aaagagaaca agtattaagg ttttctcctc agctggaagg
     8521 atgggtttca cttcctgatg aagatggttt acgagtgatt gagttcaata atccggatac
     8581 ttactggaat gagattgagc gtattggaca tataccttta cctccataca ttaaaagaga
     8641 agataaggca gaagaccgcc aaacttatca gactgtgtat gcggaagaag caggttcagt
     8701 tgcagcacct actgctggtt ttcactttac agaagagatg ctttctcaat tgaaagaaaa
     8761 aggcattaag atagttaata tcatcttgca tataggaatg ggcacttttg ttcctattaa
     8821 aactcaaaga attgaccagc ataagatgca tagtgaattt tgtaccattc cagaagaaac
     8881 tgctaatgtt attaataaag caaaattaga aggacgaaga gttattgccg tgggcagtac
     8941 atctattcgg actctggaaa gtttctggaa taatggcact ttgatgagcg gttctaaatg
     9001 gacagatata tttatttatc caggtaagaa catccaagta gcagatgctt taataaccaa
     9061 ttttcaccta cctaaatcaa ccttattgat gatggtctct gcttttgctg gttatgaatt
     9121 gacccgtaaa gcttatgaga ttgctgttca ggaacattac cgtttcttca gctatgggga
     9181 tgcgatgtat atatcatgaa aactaaactg ttatatacgc tactggatag cactatgaac
     9241 gaataccaga gactaactca attctgtaca gggactcatc aatggattat tcgtgccagg
     9301 gaacaaaaag agggaagagg aagagaagat agattctggt attcgcctga aggtggacta
     9361 tggatgactt ttgatattta ttatgatgat tttattccta cttttcctct tttcattgcc
     9421 tattgtcttc attcactttt attagagctt ttccccttag aaagcttatc tatcaaatgg
     9481 cctaacgata ttatgtggaa ggataaaaaa ttgggaggga ttctctgtga gcataatgag
     9541 tcagaaaaga aatatataat aggtttggga ttgaatacta atgttagtca agacgctttt
     9601 gatgggaaac ttgatatagt gacactatcc tcgttaatag gttttcccgt ctctaatgaa
     9661 tacctatcag atttgataat tgctaatatt cataagaaga tagacttact tacgaatcct
     9721 aaattttatc ttaattatat taatttatgg ctatatggtt tgaatcaact tgctcaagtt
     9781 caacttgata ataaaatcgt aacgggtata aatattggag tagctgagga tgggactttg
     9841 ttattaaaag atagagaagg ttatatccat gaaatcaatt atggcagttt aaactatata
     9901 cccgatttat gatagcactt ttacttgaca tttatacctt tatcaaatca aaggaatgaa
     9961 aaggataatt ttccaggagg aatgatgaaa aagtggatga taatagtcct tgcggtttcg
    10021 ctggttctta tatcctgtgc cagcaataag aaagaagttc aaccagaaca ggtccagaaa
    10081 gcagaaataa caaccaaggt tgctatcttg cccttgaaag ctctggatag ttcaagtcgt
    10141 tacattacta aaattttgac cgttcgtgac cttgaactga cctttaacaa gtatcccaac
    10201 tacactctat tagatatgaa tgagacggca gcggtatata acgagtccgg caacttagat
    10261 gtggaagata tagaaaaaga ggaacttgat aatctggcaa aacaattagg cgcagatgtg
    10321 ataattatcg ctactgttag tgaaaccaga actgatatat tcaatatttc catgcgaatg
    10381 tatagcactg tatccactga ccttaaacag ataagtttta atgtaggtaa ggaaaagact
    10441 gcccgttgga aagcgctgga agagtatatg atgaaggaac tggatgattt tgtttccaac
    10501 gaaattgata agctgttcaa tatcgctgct aatcacttta ataatggcaa ttatactact
    10561 gcagaacaaa ttttaaaaca ggttatagct ttaaaacctg aaaaagttga tgcttactat
    10621 tttctcggta atacctatat caaattagag aaaattgaat tagctgaaca aaacttccag
    10681 aaagctttag agatagatcc acaacatcaa gccagcctta tagctctaat tgatctctat
    10741 gataaaacca atcaacctat gaaaaggatt ccattaatgg aaaggattgc ggaagaaact
    10801 cgggatgctg aaacctggtt tgctgtagga aatctctaca atcaaattgg caacaaagaa
    10861 aaagctaaag aatcataccg taaagctctg gctattgatc ctgagttcac tggagctatt
    10921 gtaaatttat ctattattct ttttgatgaa ggaagttata atgaggctat accttacctg
    10981 gaaaaagcct ttgaaaaagc tccagacaat acttttatag ctgcgcgttt agctactgct
    11041 tatcagagaa gtggaagaac tcaggaagcc attgataaat atgagaatgt gttagctaag
    11101 gatcccaata atattcaagc ttatctcaat ctgattagta tatatagaag taacggtaac
    11161 aacaccaagg caattgaaaa gataaatgct ttgaagaaag ttgctccaga taatccttat
    11221 tcctatctta atcttgctgc catttatctg gaacagggta aactaaatga tgcagaaaca
    11281 aatgccaata ttactattag taaagaccct accctttacc aaccttacat aattctttct
    11341 tctgtctatc agagtagagg tgcagagtct tacaataaat acattgattt agaccgtcag
    11401 gcttcaagag cggttggaaa gaaagcttta gaccttaaaa agcaaaggga cactgccaag
    11461 actacggcaa taaatcatta taataaggca ttaaactatc tgcagactgc tcgtaattac
    11521 actgatgacc ctgaagtttt aagtgatatc aatagtcgta tcgcatatat taatcagcta
    11581 cttgcacaat tctaagaaca ttctgattat atagcataaa ataataagcg cctgataaga
    11641 atcaggcgct tatttctttt atggagtttt tcttatgaat ttctttactt aaaagcttcc
    11701 ataccagaaa agtctctttc tggattaaaa ctttgaagaa tatgtaaatc actttccgtt
    11761 agttcatcaa gacagatacg tgtaatcaat tctcccaaac caggtcccag cataaaaccc
    11821 tgtccacaca ttcctatagc attgataaga ttatcacctg attttcctac tataggaaag
    11881 ccatcgggag tcattggata ttgacctctc caagttctac gaactcttaa actatgtaat
    11941 cttggatata cttccagcat tcttttggta cacataggta aaaagacaga agtacttcta
    12001 ttgtcaattc ctgttatcgg tggtttaggt gtgatacaaa aaactatctg tccttcatta
    12061 ttttgataga aataaaagtt ttcagaacca ggacgaattc gcatatcaac taccatagga
    12121 cccataaatc tggctacggg ttctgttatt cctgcttcat gattttcagg ttgaaccgga
    12181 agattaagtc ctgccatagc agcgatttct tttccataat ttcctgctgc attaattacc
    12241 attcctgtag aataagtccc tttatctgtt ataacttttt ctattttgct ctgactcata
    12301 tcaaaaccta taaccttttc cctgaaccga tattgaactt tagcttccag agaatgaaaa
    12361 taataagccg aacctaaaag taggggagag caactaccat cttcaggaga ataagtggaa
    12421 cctctcaacc cttctttatt tattccaggg acaagttcat tatattcttc aggactaacc
    12481 caggtgatat ttaagccata gctatgttgc accttcatca agtcctttaa agtcttttca
    12541 tcttcttcag tataagcggg ataactataa ccattgctca tccagccaat atcatcaccc
    12601 caggtttctt tccaggtctt cattatttca atggagcgct tgcaaacatt gatttttcca
    12661 taatcactat gagtagcacg aactccacct atagcttttt tattattttg ctgacctggt
    12721 gcactttctc catctataac caacactttt tgtttttgta aagccagatt taaagctgtg
    12781 ggaaccccta tagagcctgc tccaattatt attacatcat atatattatc cattttcagt
    12841 ctccatcagg aaatttttct aagggaacct caatgaaaag agggcgtttg gtattaggaa
    12901 caaccttttc tattggtata ccttcttccc ttaaaagttg taagattaag ttctcacaag
    12961 tttttgcacc acagggaccc attcctgcac gagttattgc tttaatctga tttagattag
    13021 ttactccaga gtgtatcagt ttacgaatat caccagcttt aactctttca cataaacata
    13081 tcatcgcctc atctggcatt ttatcaggga tcacaggttc tggaagaggt tcactgactt
    13141 ctttagcctg aatacgaaat gatgctattt tgcgggcaat ggctttagga acctgcactt
    13201 taatcaattg agtgtgccgt tttttcacat ctattaccga tatgatagta tagaagccca
    13261 gagaattgcc ttctatatcc atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13381 nngggaaaac tcctgtctct ggaaggtctt tctgataatc ttttattgct ccaggatggg
    13441 acttcaacac ttcagcttta gcataccatt cggaaggaat ggcttcaact tcatcttttc
    13501 cacattcttt agctatagta agtccggcaa tttttccatt gaacattgct gaagatgctt
    13561 ctgcaatttc ttgtgcatct cctgctgcaa aaacctgaat tcctgcttct ttagcttctt
    13621 cagtgaattc tgaaagggaa ttcaatccaa ctgcaatcag gagtgtgtcg caagcaaagc
    13681 ttttttccgt gcctggtata ggatggaatt tactatctac ttctgcaata gtaattcttt
    13741 ctactgtctc tttaccttca gcattgatga tagtatgcga agtgtaaata ggaactccca
    13801 atcttttcaa tttgtccgaa tgaactttat atcctccgca ttgaggcata gcttcagcta
    13861 atccaactac attaattcca gcttgtaaag catgatatcc tgcaattaaa ccaacattcc
    13921 ctccaccaat aataaaaagt ctattcgttg gacgaaccag atctctattt accagagtct
    13981 gaaaagctcc tgcaccatag atacgggcaa gattattgcc aggaaagcgt aagaactttt
    14041 ctcgcgcacc tgctgcattt aaaatgatgt gcggagtgac aagtttatat attccatctt
    14101 ttaaaattcc cactttcttg tcgctaaaaa caaatagggc agtgctatta agccagactt
    14161 taacggagga atattgctgg agttcttgag ctaataattt cccaatatca atacctctaa
    14221 ttccagcatt gctatcttct tcactaccaa agaatttatg ggtttgcaac acaagcttgc
    14281 caccaagtgc ttgtttatca tcaatcaaca gagtatcaat attatatttg cccaattcta
    14341 tcgcagcaga aagtcctgca ggaccacctc caataataag aacctctact tccaattttt
    14401 ccaggggagg aaatttgggt ataaaaggag tttcgggaag ttcaggaagt ccttctaaag
    14461 aacgaacttc catcccggaa gtgacagctg tcatacagga tttgaccgct actccatctg
    14521 ctataacggt acatttagca cattgtccat ttgcacaaaa aattccttga gcgctgccat
    14581 ctttatgatg atgtccaaaa acatttattc catttgcaat aagtgccgaa gaaatcatct
    14641 ctcctttttt tgctaaaagg ggttcaccat tccagctaaa taaaacatct tctctttctt
    14701 ctatcggaag aatgggatgc tttaatatac ggtgattttc cataatatga gagaaaactc
    14761 cttttttgtt ttattttttt aaatccaaaa ctttaaaaac ccacttatgt caagcaaaag
    14821 ctgaagagaa aaagctttga tatgagtaaa taatacttct tcttcctctc ttctttcttc
    14881 cttctttcta tcttttaaaa aacgttttcc tttccttcct attcattccc agattttctt
    14941 tagcttatgt ccagcttatg tccactttcg gacataagct ggacatcagt tggacatcag
    15001 ctggacataa gctggacata acctgtgcat taacacgcga gatgtagcca atttaaagaa
    15061 aaagtattat ctgattggaa aattcaatat tataactatt ataaaattga atattatcca
    15121 gcgataaaaa caataatata agggaaacaa attatcagat tatcagtttt atctactaaa
    15181 tatatgaggg attatagact gtttaaatca aaaaaatcag gaagaaaatt tattcattct
    15241 tcctgatttt attaactcac ttaagttaat atttatattt ctcaatattt tacttcatta
    15301 agaccatacc cttaatctga ttgtctttcc cattggataa agagtataaa tatattcctg
    15361 aggagacagg tttatcatta gagtctgtac cgtcccagat aatattatga gttccttttt
    15421 ccaaatttcc tttaaataaa gtttttatca gttgtccctt gatattataa atatttaaag
    15481 tataaggaag agttatatca ttaacaagaa acttgatatt agtggaagga ttgaagggat
    15541 taggataatt ttgaagtaac tgaaagttag agataaaagg agtagaggga tcatttatag
    15601 gagtttcatt ggatatcttt tgcgcataca atgaattgta tccatcttca aaggcttcat
    15661 tagaagatct ataatcactc cagataatat aagcttcatt attatgtatg gtcaatttag
    15721 gagaattctg attattgaat atattacaaa gaacataacc ttctgagaca cttcccccaa
    15781 cagttccatc attattaaga taattataat atagattatt gtcataataa gtagaatatt
    15841 gaggactttc agtccaagca acgatcattc cgccattatc aaaacggcct ataataggtt
    15901 caaattgatt ttcagggttt tgaattatat aaataccatt ttcattccat aaaggtaatc
    15961 cctgcaaaga aaacttttga gctttaatat ccaaattgtt aatataatta tcccagacta
    16021 caactatttc gttttggaag agaggatttt ctacaacaga ataattattg atataagcat
    16081 ttgttaatac ttccattccc tccggacccc aaagtctttc tccatcagag gaaatatgct
    16141 gagcaaagta aaaaagatta ttactattat aaacagattt tgtccaaagt acaaatatcc
    16201 cttcagaagt taaagtaact aaaggataat actggtaagt ataaatatca ttggacatag
    16261 aaacagatag tccatattcc tcccaaccag gtgaagcttc accatttgga gcaacttttt
    16321 ttacaaatat tttttgataa aattgagtat aatctaccgt agtccagata aaatagttcc
    16381 cttttacatc attaatataa aattcagtag aattttcatc agctccatca ttaacagaga
    16441 ttaatattcc atttggaccc cattgttttt caccattata taacatttga ccataaacgg
    16501 aaaagggagt aggattacca ttttgataat caaagctgga ccaacctata tatacctgat
    16561 ccagatctgg caaatatgaa atatgaggat tgtattggta aaaaggatta ctttcggtta
    16621 gttgtattcc atagtcaccc cagagtctat tcccattaga atctatctgc tgtagataaa
    16681 ttttgtcatt cccatttcta tcatctaccc aagcaaaaat aatactatca tctggtaaaa
    16741 ctacaacaga tggggaaagc tgatttcctg aatagggaag agttataggg cgtccattcg
    16801 gttcaagttt tagagagcca tcagaattaa gaaattggaa aaagatttta taacccaaag
    16861 tttcgtagtc tctagaatca caccacataa caaccacatc ctgacttcgg tttgctatta
    16921 agggtctatc tattccacca ccaatacccc agaaaatcat cttaccattt tccaccaatt
    16981 gagcgatatc gtttgaatta taaacttgat agtatattcc agggctacta tttcgttcat
    17041 cactccaagc gcagtaaatc atatctatat taaatataag aattggattt acttgcttat
    17101 aaggatcatt acatatgatc ctgccattgg gttcccatag tggttgtcct gaagttgata
    17161 gatgttgacc atagatatca aaattttctt ggggtaccga atctgtatta cggcagtcan
    17221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc tgaacattat ttcaccattg
    17341 gaaagtaaag aagcttcaat attaagatga taacccggtg aattatcaag aactacaggt
    17401 tcattccatg ccatagaccc atcatataga taacgattaa tataaatatt ttggttagtg
    17461 ccatcaaagt ttggataggc aactattaaa ccatcttgac catcagataa ggcattataa
    17521 taataattaa aagaattatc attatccagc tgaataccat ttatatccca gattggattt
    17581 ccttgagaat caagatgctg tccatataat ttccaataat agttacgata atcgttccat
    17641 aaaatataaa ccccgcctga attatctgat actattttaa tataagtctg gttctcatta
    17701 gcaatacaaa caggaattcc tcctgtttgc cagagtagtt gtccctgaga gtttatcttt
    17761 tgggcataaa tatcagtacc atagggatca ttcaaataat caatccaggc aatgacaaag
    17821 ttatcatcac tggtctgaat tataacaggt tcatcctgat ggttaagttt cccatcgata
    17881 agagctggtt cattccagat tatattgccc tgagcatcaa ccttctgtgc ccagatatca
    17941 cgatcacccc ttctggtatc actccagaca tagataacgc caccatcaga agttttagca
    18001 cctgaactat accattcaat attagcaccg aggcgaatag gtacctcatt ttcccataaa
    18061 gtggcagctt ttaatgagaa tagaagacaa agcattatac caatgaaaat gaactttttc
    18121 attactatct cctttttaaa cctgataaat caggtaaata tatctacttt tttatcctta
    18181 gctaagttat agctactata tatttaaagg tatggtttat aaatattatt actttatcaa
    18241 gaccatacgc ttaatctgat tactttttcc attagataag gagtatagat atattccaga
    18301 ggagacagtt tcacccttac tatctgttcc atcccagata atattatgag ttcctttttc
    18361 cagatttcct ttaaccaggg ttttcacaag ttgaccttta agattgtaga tatttagagt
    18421 atatggattg gtgatatcat taatggtaaa cttgatatta gtggaaggat taaaaggatt
    18481 aggataattc tgaattaatt tgaattggct aatagaaggt gtagtagggt catctaaagc
    18541 tacaatttca ttagaaagtt tttgagcata gagacccaga atttcggttt ttccgctgga
    18601 tcttccatca gcccatatgg caaatgcttc atttccgaca atagcaatca taggattata
    18661 ttgattcttt tttgcatcgc agaggacata tcccccggga ttatcacata caaaagttcc
    18721 atcgctttta atatatttat aattaatgtt ggattcttcc gtgatagtat tataataatc
    18781 agtccaggct atcaccatac caccattatc aaaacgtgcg agatatggag aagtttgcat
    18841 gctatctttt tgcaccacat aatttcccag atcaccccat tgagggatac cggtaagaga
    18901 atatttttgg gtaagaatat cacccattcc agctatattt tcagtccaag caaagacgat
    18961 ttcatttcgg tataggggat tttctgcaat agaaacattt agctgttctc taccataatc
    19021 tgcaagattg acccctaagg gatcccagag tctttcacca ttggaagaga tatgttgtcc
    19081 ataataattc tctatgtcat cgccacgagc atctttccac atcacaaaga taccttcatc
    19141 tgtcagagta gagacaggaa atctctgcct acaatcccaa ttagtaatag gataggtgga
    19201 aacttgtaat ccagattcgg gccaaccctg agcagtttga ccatttgcat caactttttt
    19261 agtaaaaata ctctgagtaa attctattaa attagcatcc tgccaggtaa aatagttttc
    19321 cttaagctca ttttgactgc attcgtggta caagaattcc tcttcaggat aataagatat
    19381 ttttatacca tcaggtcccc attgcttctg ttcgttttga atcatttgac catatacata
    19441 aaagcgtaag tttccactat gagattcata attagaccag cttatataca cctggttttg
    19501 atctgcttta taggatacac gtgggttttt ttgttctatt gggatacttt ccgttagagg
    19561 aataccataa tctccccaaa gccgattacc gttagcatca agatgctgaa gataaatatt
    19621 tggattttcg tcgcgagcat ctacccaggc aagcacaatg ctatcatcag ataaaactgc
    19681 agctgcggga ttagattgat tagctccagt agaaagcgtc acaggacgtc cattaggttc
    19741 taaatctata gaaccgtctg aattgagaaa ttggaaaaat atctggtaac ccatattagc
    19801 aaatctggta tcctgccaga taataacaac atcctgacta cgaggaagaa taagataatt
    19861 gttcagaggt gtatcaccac ttaaacccca gaacactaat tcaccatttt caggtaattg
    19921 agcaacacca gctgaattat atacctggta gtagagacca acacttccat tacgcatatc
    19981 catccaattg ataaacacca tattattgct aactttaacc agagcactat tttgctgatt
    20041 aggagcattg catatgggtc tgccgttagc ttcccataat gctgtgccag tggcagaaag
    20101 atgttgacca tatatatcat cattgggggt atcaccattt cgtaaatcat cccacacaat
    20161 ataacaacca ccattggaat ctggagtaag acgcgcttca ttttgagcaa attcagctac
    20221 gcataaagga actccatcaa catcccacaa aagatctcca gcagcattta ttttttgaac
    20281 aaagagatca gttgcataat tatccaatct tttatcttcc caaacaataa tcacagcatt
    20341 atcacttgta gctacgattc gtggattcaa ttgaggagca aagtttgccg tccctgaatc
    20401 tccaaaaaca ataaaaggat ctcgccagag tatattgcca tttatatcaa ttttttgagc
    20461 atagatatcg ggatcttcat ttcgttgatc ttcccataca aatacgaatt caccattagt
    20521 taatgtagcc atacgcactt taccttgatt tccaggggca ttactgataa tggtaggttg
    20581 ttgccaagcc attgtcccat cagaaagaaa acgcttagca aagatatctt caagaccacc
    20641 aaaagaataa gtgtaggcaa ttataaatcc accttgacca tcaggcaaca ttgtgttctg
    20701 cacttcatcc ccaatatcat tagcgatagg aattccatta acttgccaga gaggagcacc
    20761 tgaagagttt aaatgctgtc cataaaggtc tttacctgga ttacgagaat caacccagat
    20821 aatataaaca cccccttcat tatcagattc catattaaga gaaatttgca cattaggata
    20881 ggtgcagaca ggtacaccac ctgtttgcca taataattga ccctgagcat ttattttctg
    20941 agcataaaca ttaccatcca ggtcatcgga aaaatcaatc caagctataa caaaattatt
    21001 atctgaggtc atagttataa cagggtcttc ttgacggtct ggttttccat ctatcaaaag
    21061 aggctcaccc catactgcat taccttgagc atcaactttt tgtgcccaga ggtcacgttc
    21121 tccaagcttt gtatcactcc aaacatagat tgcacaacca tctgcagttt gtgtgccaga
    21181 gcgaaaccat tcaatattta caccttgccg gatagggata ggttcctgcc agagcagttc
    21241 tgctgaaaga ttaataatta atcccattag cagtaggaag aaaattgact ttttcacagg
    21301 taactccttt ctgatttgta taacacatta attttttata cagatataat taaatgcaat
    21361 ttcctttcca aagaagggaa ataagaatta aataatatta acgagaataa actgttctac
    21421 ccgctataac tgtttccata acgggaaatt catatccata gaagcatata gccgagatca
    21481 aactatcttc tggtatgggt tcaggaggta atttcagaaa acagagatct gcttctttgc
    21541 ctgaatccaa agagccaatt ttatcctggt aacccaacat ggttgcatta cctaaagtga
    21601 tatgatagaa aaggcgttca ggagaaattg aataaacact ctgtctgtag tttgccatct
    21661 tagaatgata tagcatattc aaagttgtac cagcagcaac atcacttcca acgcctattt
    21721 taagatttct ggaatatagc gcttcataat taaattcacc actttttaag aaaaaattag
    21781 aatctggaca gtgagctatt ttgcaatcgt attttgccaa gatatccatt tcagctggag
    21841 aaaggtgaat gcaatgtgca agtatggtat gaggagttaa cagtccagcg ttttcatata
    21901 cctgagtgta ggtatccgtt ccaaataatt gtataacatt agctatttca atctgatttt
    21961 cggaaagatg agtttgaatc caggcagaat tatcctgcgc aaatctggca atcttttcta
    22021 ttaaattaaa agaacaagat atagcaaaac gaggtgagaa aatataatcc tgaagaggac
    22081 taacattatg atatttatga tataattcca cgcttttctg aaagacaaaa tcggtgcttt
    22141 gcagcaattc agaaggggca ttttggtcca ttaaggtcat ccctatcaag gtacgcgcac
    22201 caaatttttg tgctatagaa aaagctgtct cacaagcttg aggataggga gcagtataga
    22261 taacggaagt agtagttccc gcggcaaaaa gagcttgaaa gaaatcagtt gctatttttt
    22321 ctgcaaagtc aggattttgg gaaagagctt cttcaggaaa aacattcttt tctaaccagg
    22381 aaagtagtga aggttcatat ctgcctctaa tccgatattg agagagatga gtgtgaatat
    22441 caattaaacc gggaaggcac agttcatcca gcctttcttc acaatctaag tctacacctg
    22501 attgataagg atggatatta ttaattatac catcttccca ggaaataaca tagtccaatc
    22561 cgataaaagt atagtcagga ctaactggat ttacgatatt agttcttatc tttcgcatat
    22621 ttaacccttt ctatggataa aacagtagca ttacccagac cgaagtgcaa ttcctgagca
    22681 ttttgtgaag ttgttccttt agctgaagct aattctctta ttaaagttct ttgtttccca
    22741 tcagcatccc gtaaaattaa ttttaccctg gtgccaaaag caggggaagc aggaaactga
    22801 ctgaacttta ctggattatc tattaaaatg attttatcat cctgccaaac tggtttaata
    22861 aatatagagt tattagtagt ttgagtctta ttaagaagaa tcttgtctcc acttccactg
    22921 gctataacaa gatcagttaa cccatttcta tcaagatcag cagtggcatt accccaacca
    22981 ttataaactc ttgcgcccgc aagaaaggtg atatcagtaa aagtgccatc tccattattt
    23041 ttatatagat aggagcgttc atttttatag attgaagtta taaataaatc cagatagcca
    23101 tcattatctg catccagcca gagtggatca gaatgacatt catcataggt gataccagct
    23161 gatttagtta tatctgtaaa ctgccagaac cagagagtgt cagattcaat aactttataa
    23221 ctaagtccgt catttctaag taattgactg atatcggaaa attcaataaa gcgaggatga
    23281 gcaaggtttg caacaaacaa atctaaatct ccatcattgt caaaatcgcc ccaatctgct
    23341 ccaatactgt gtccataata gccatcttga tagtgcccag ttaagccata aagtgcagca
    23401 acatcaacaa aaatggaatc tttttggtcc cagcaaaagt ttctattcaa cctataattt
    23461 gtaaccagaa tttcttgttt tccatcatta tcaaaatcag aaggagcgac acctctgcca
    23521 gctaagcctg gattatctgc ataggttgga gagcgaaaac ctcgttttac actttcatct
    23581 ttgaagtaac cgtggtcatt ataccaaaag aaatcaggat aaccgcttct aatttgccat
    23641 tgctcataat tagcacaata tagactggga taaccttctc catcaatatc aatccaggca
    23701 gcaccttcag ttggataatg gtcatctaca tctccagcac gttcgttgac tttaacaaag
    23761 cgaagacctt cttgattctt cattagagat tcacccaaac cttcagaatt atgagagata
    23821 gtaacaaaat ccagataacc atcccgatta aaatcagccc ataatgctcc atttgcccta
    23881 agattagcta cattcatagt atcactaact tcagtaaaac cctgtcctga atcattatgc
    23941 cagagtgaat gtccgtcaaa aaggaggtca ttataaccat cgttatcgta atctccaata
    24001 gcaattcggg aatagtgttt atcagggaaa ggatattcct caaagattat tccatcataa
    24061 ttcataatct catgtagcca ggcatatagt tctttatcga catttagctt atttctaaga
    24121 aattccaaat cgtgaagaca ggaatcatca tattttttcc gcggtgaacc ataaaccaga
    24181 gcagttgtat attctttagc tgctgtttgc agatattttt tttgatttat gagggcatta
    24241 attttacctt gccagtaatg caattctgcc agttcaccac gatcattatt atcaaattgg
    24301 ataactgaaa tgtcctccaa gagttgtaaa agagcatcat taggttcatt aaaaagtgca
    24361 taaagatttt cttcatcacc ataaagattt aaatctgcca gcaggctata atattctcct
    24421 ttaaccttgg ttaataaaag tctattacgc cattgttctg gggtataaag atcataaacc
    24481 acacgaattt cttggtcaga agagtaatct tttaatgcag aatctaacat ataaatagct
    24541 tgattgagaa tcaaacggtt atttgctaat tcctgattcc tacgataatc tggacttaaa
    24601 gtataaagag cagatatgta taaacattca gctgaacggt atccttgttc ctgtatacat
    24661 tgatgcatta tatcatattg accgaggctt aaataatggt ataatttata aaagtaggtg
    24721 gcttgagtcc attgtgaaag ggggaaatga tattcaaaac gattaatcat agccagagcc
    24781 agagaatcgg aatattccga actgatttca tctatccaac ctctggcaat atcttcaatt
    24841 atctgattat aacctcttaa ttgaagaatt tgttccagaa attcttcctg tgtgatttgc
    24901 ccaaaataat aatccagagt gattctatcc tgaggattag taaaaacctg actgagcttt
    24961 agatacatct cttctatttg ctgattatta agctgcaatc tttcagcagt atttatccat
    25021 ctgagagcat tctctaattg atgatatttt aaagctaata tttcgttaca cttaagttca
    25081 agttcgggtt tttccgctcg ttgactgtat tccagaagca aacttagctc ttccttacct
    25141 ccttcctgcc acttttgcca agaaagacta tacttttcaa taagggaaaa ttgctcagta
    25201 tccattaacc atctaaaatc gtcctgagtt atagcaaata ataagctaaa agaaaggaaa
    25261 caaataatta agaaccgttt cattttttct ccacttcgga tataatttct tcaatctcag
    25321 ccgcactaaa atgccaggta gcattacaaa attgacatac aggatcaatc ccatctctca
    25381 attcttttaa ttcatccaga ctcaataact ttaaagccgc agcaaatctt tctttggaac
    25441 aatgacaacg atatctaact ctatgaacag gatagagctc taatccacct tctttaaata
    25501 caaaactatg caatatttca ggtagataca ttcccatatc cattaaatct gaaagattgg
    25561 gcatatgatt gatgttatca attaagcgat ccgcatttaa ccggtcagca gagggtaatt
    25621 gctgaatgat aaatccacct gaagcacgaa ctttagcatc tttatcaatt agcaccccca
    25681 aatttactgc agttggtatc tgttcagatt gctcaaaata atatgccaaa ttctgagcca
    25741 catctccttc aatgagtgga gttaaacctt tagtgggacg atctccaaga taatatcgga
    25801 tcacagttaa ggtgccatta cccaaatact taacaggatg aaaattctcc tgcaagtcat
    25861 ccaaccaaaa ttttggctca aaagcataac ctcgcacatc accttgattg ttacagatgg
    25921 tcagggcacc tttcaagggt ccatctccat caaaacgtaa agtaacttct gcaccttcat
    25981 cttttaaatc cagactgagc atagctacag ctggaatcat tttacccatt aaaatggtat
    26041 ttataggtga aaggtcatgt aggtctcttg cagcttgaac cgtattggta gaggatacgg
    26101 ctaagactcg gaattgacca tttaaagcca ttcctctcca aagataatca ctttccttca
    26161 taatacatcc aatctgttga aatttgagtt cctggataat aaaaattgag aatatcccag
    26221 taggttttac cttctcttgc ctgtcttaaa gctccagatt gacacattcc aactccatgt
    26281 ccatatcctt tgccttttag tgtcaggttt cctcctggaa ttatctccac ttttccattt
    26341 ctattgaagt tatactttcc gtcaatatag aaaaaagaag aacgcaatcc tccaaatatt
    26401 tctctaattt ttgcttcccc aaggattctt gtctctcctg aattgtaaaa actcatatca
    26461 ataatccttc cagaaatact acgctggttt atgacaattt tatctatatc atccattcct
    26521 gcattacttg ctaatttctt gcgagaaata gtatgactcc agcacattcc tgctttttcc
    26581 caggaattca tcatagggtc taaagtatct gtatctatcc attgtcttgc ctgattttcc
    26641 tgagttaaat ccagactgtc aactcctgct acgcaaggaa ctcctaataa atggggaaga
    26701 ggttgactaa tccatatttg atgagctgca tctgtgtgtc ctccacaatt gctatgatag
    26761 ggtgtatctg ctattcttcc gtttacagtt aaaatctgat tggaggtgct atcaaccgca
    26821 atttggacat tgggattatt tataaatctt cctttatata cttgacaatg agtggaattg
    26881 caaagatcat atccatcgtt tttatgccgg ttaaaagtta gaagtgcaat agaatgggtc
    26941 ctggctgtaa ttgcttgagc tttcattgct tctattggag cattgccacc tatttcatag
    27001 ggaataaccc cggctatata ttcttctaaa tagagcaact gggtaaatac caatctatta
    27061 tctatctcct tcagataaaa ctcaccacta taagcttctt tttgtgaatt tactattatg
    27121 ggaaaatcac tatgaattct aagtggcgtt tccagataat acttttcttt tctggcagta
    27181 gttatcttca cagtagaacc aactataggt atgtattgca ccattttatc tgggataccc
    27241 tgtgctctgg cataagctaa tgcactatca cgagatacaa aggaagtatc tgcataaatc
    27301 aaattttccc tctttagcct cagatgattt tctaaccaaa caaatgattc ataaggtatg
    27361 ccctcagaat catctgtcca aggttcagtt ctggttaaaa ttccccagca agcctgttta
    27421 tctgttttta tcagctctat ttccaattta ccattcatct ttctgcaaac cggtaaatct
    27481 gattcataaa tgtttattgt actttctgat tctttttctg gttttagggt tatcttatta
    27541 tcaacttcaa ttaaaacctc aagatatagc tgtccatttc tgatttctgc agctctcaaa
    27601 atagggttta aagacattag ggatatgaaa agtaatgctt taactaatgc tctatatctc
    27661 attgatatgc ttataattgc tatttaacaa cggaacctgg aggtgaatat ttttctggaa
    27721 ctgctaaagt aagattccct tctgattcaa tagctaaaat cataccttga gaccttattc
    27781 ctctaatcgt acggggctca agattaacca gcattaaagc ttgttttcca ggtaaatctt
    27841 ctggtttata atgttctgca attcctgcga ccagttctcg ttcttcattt ccgatatcaa
    27901 ctttaagatg taaaagttta tcggttttag ggaccttttc tgcagaaagg atagtcgcaa
    27961 ctcggatatc tattttagca aaatcatcat agctgataaa ttcttttatg ggaatttgat
    28021 ttatctttga agaatttagt ttagcatttt gttttaattt tttaacttct tcttctataa
    28081 ctgaatcttc cagcttaaaa aatagaggtt caatcttatt taaattataa ttggatttta
    28141 aatctatttc ttcccatact gaaagctcag gcagtcccat cattttccta agtttattca
    28201 tactttcagg cattacagga tataaagcta ctgataccat acgcaaaagt gtaagacaaa
    28261 catacaaagt ttcttggacg gaatttgaat ctttctttat ttctgaccag ggttttcgtt
    28321 catcaaagta acgatttccc aaacgagcta tatccattgc ttgtttagta ttccatttaa
    28381 cctgatagtt tttgtatcct tcatctattt gcttataaag ttcctgtgct tcttccagtg
    28441 tctttaaact gctatcagag aattcaatag gttccagcaa tccattaaaa tgtttattga
    28501 ttaaggtgaa tactctattt gcaagattac ctaaaacatc gtttagctca cgattgattt
    28561 tctgttgaaa ctcataaaaa gaaaaatcac tatcattttt ttctggagca atgcaagcaa
    28621 gatagtagcg tagatattct gccggaaatt tttctacgaa ttcatccacc caaatagccc
    28681 agtttctgct ggtagaaatc ttttgtcctt ccagattcat aaactcattt gccggaatat
    28741 cataaggaag acagtaagct ttatcctgac ccataagcat agcaggccaa atgagagcgt
    28801 gaaagattat gttatctttt ccgataaagt gtatcaatct ggtttcagga tcaagccagt
    28861 aatctttcca gcgattaggt tcaccaattt tttctgccca ttcctgagta gaagatatgt
    28921 atccaatagg tgcttcaaac catacatata gcactttacc ttcagcacct tctaaaggaa
    28981 ccggaactcc ccagttcaaa tcacgggtaa taggtctttc caccaaacct tgttccagaa
    29041 gattcagcat aaaattgcgg acattttcct tccagtaatc ctttttgcta atccaatctg
    29101 caagctggtc tttaaaatca ttcaactgta aataccagtg agtgctttct ttgattacag
    29161 gggtgtttcc acagatttta catcgtggtt ctattaaaga ggtggtctca tagatttgac
    29221 cacaggcatc gcattgatca cctctcgcac cagtagcatg acagcgagga caaattccct
    29281 ctacatatct atctggaagg aatctatgat cagtctcaca atagaattgt ttggtttttc
    29341 ggggtaatat atgaccattt ttatataagg ttaaaaagaa ttcctgggaa agtttataat
    29401 gtcccggtct tgcagtgcca gaaaaattat caaattctat ttccagtgcc tcaaaagcat
    29461 tttttataga ttcatgatag cgttgaacta tttcttgagg actaactcct tcctgatctg
    29521 ctgctataga tataggagtc ccatgttcat ctgttccaca gatataaact acatcttctt
    29581 tatgcaatct tagccagcga acaaagatat ctgcaggtaa gtaggctccc gctatatgtc
    29641 caatatgtaa tcttccatta gcatagggta gagcgctggt aactaaatat ttcatttttc
    29701 acatccgata aaagctgaat gtagaatctg aacacaatct ttgacttctg aactgggtaa
    29761 cattatttct atggtcttgt cactatgctg aatacgttta atatgaaaag gttggcaaag
    29821 ttttaaagta tctgctaaaa aacttggatc aagattaatt cctaaaccta ccagagtgac
    29881 ataaccaatt ccattttcag ttttgagaat aggtatctct ttctgagata gaagatatcg
    29941 gatttcttta tcatatttct cttcaataat taactctaac tgattctcta tcgctaaata
    30001 cttaaaaatc tcccagttcc agatttttaa taaatcaata gtttccgcag tctcaggtat
    30061 tatatagctt aacaggtttt ctttatgcgc tatagccgtg atatgtcttt cttccatatt
    30121 tttttcctct tcaaaacttt cttcttgaat aagcattgtg ccaggtgcaa aagtgaagga
    30181 actctttatt tccacgggaa tactatattt atatgcaaat tctactgccc tgggatgaat
    30241 aactttagag ccattataac tcaaagccag catctgggaa taatctatag caggtagtaa
    30301 ttttgtattt gaaactatct tagggtcagc tgtgtagact ccatcaacat cagtatagat
    30361 ttcacatttt tttgcttgaa gataacaggc taatgccact gcagaagtat cagaaccgcc
    30421 tcttcctaaa gtggtaattt ctttgctttg gctaactcct tgaaatcctg ctacaataac
    30481 tatcttacct ttatccaatt cttctttgat tcggaatgca ttcacattca aaattctggc
    30541 attgccatgc ctttcatcag ttataattcc actttgagaa ccggtaaaag aaatggagga
    30601 ataaccttcc tcctgtaaag ctaaagaaag aagagacata cttatccttt ctcctgctgt
    30661 aagtagcata tccaattctc ttctggatgg atgttcactt atttgatatg ccagatttat
    30721 caggtcatcc gtggtttttg ccattgctga aacaaccaga accaatgaat caccttttcc
    30781 tgcttcaaga gcaatcctgt gagcaatatt gcgaattaat tctatacttc ccagagaagt
    30841 accaccaaat ttctttacga taatagccat aatcttttct taaatccata aaccagcctc
    30901 ttgtcaagat ttttcttatt tctac
//
LOCUS       DAORZF010000015        30126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_1303_length_30126_cov_372.673672, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000015 DAORZF010000000
VERSION     DAORZF010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 30126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30126
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1303_length_30126_cov_372.673672"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="PK015_03015"
     CDS             complement(<1..667)
                     /locus_tag="PK015_03015"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HOL33478.1"
                     /translation="MSENKEYYAILNELISKNQTVWQVTIISSIGSTPAKPGMKMLIP
                     LSGKVYGNLGGGELEHLLISWVRKEQPSSPILETYLLSEEGEKPERTEGIPLSMVCGG
                     KVSVFIEPLFSSNILYIIGAGHCGKALAHLANLCDFQTRIIDNRPEALENIPPEICND
                     LHLSDYKDLEKHIQFNQNALIVIMTQGHIYDEQVLEQCLDKPFRYLGMIGSKRKAEKT
                     RQML"
     gene            complement(680..1498)
                     /locus_tag="PK015_03020"
     CDS             complement(680..1498)
                     /locus_tag="PK015_03020"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOL33479.1"
                     /translation="MRNINHFSQSKFSSLSPVHQLKALDKLVSSLSQSLSQNNNYFEL
                     KEHIIHCISWMQSPLPECVSQLSQDLQQEESFDKIYNALVQYQRARGKTFKDYQIQVR
                     KGDGLKVVDAEAQRKAQQIILILDNLRSAFNIGSIFRTAECLNLQAIYLCGVCATPES
                     AALKKTAMGTEDKVQWKYFPQTEAAILSAKSEGYYIYALETAAKAKSIYEVNYQFPLA
                     LVVGNESLGISQDALKLCDSFLAIPVQGWKNSLNVAVACAICAYQIVWGNLPQK"
     gene            complement(1495..2085)
                     /locus_tag="PK015_03025"
     CDS             complement(1495..2085)
                     /locus_tag="PK015_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOL33480.1"
                     /translation="MIVEEVKKAVFLDRDGCISPDNFGLKPDPEKYHLYPYTIEALRI
                     LQNLGYMLIIVTNQAGIAKGYFDLETLEKVHAKLRELLNNEGIILTDIYYSPYLETGT
                     VPPFNIAHEDRKPGLGMFYKAMRDHPFDPNKSWMLGDRKSDVYFGKKAGLKTVLLLSG
                     HGKEELLTGFEGWEYTPDFIAENLLTAAKLIQMLTK"
     gene            complement(2066..4474)
                     /locus_tag="PK015_03030"
     CDS             complement(2066..4474)
                     /locus_tag="PK015_03030"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HOL33481.1"
                     /translation="MNKFILILLILSFPLSLVLLAQNERDVFPSKLPEVVPDTLTQAK
                     VKEDSLFYSADSVSYYQNLEQIRLYGNTNIRYQEFTISSDSIVINLKTNRAYSVGNTV
                     MQDGAQILLGSNVAYDIETQTGIMENGISSMDKSFYSGKQVRKIDVETYDVDDCNFTT
                     CEYEEPDFWFTAKQVRIYNKDKIVGKNVVAYVNHLPIFYFPFITIPLERGRHPGFLIP
                     VPGYNVVDGKFLKDIAWYYPYKDYADIIVSFDLYEKTGWKADLNLSYLKRYILNGNLN
                     AAYQKKLTDIQTYNDWSVRANHHQELGNKATFDLNVDFVSNKRIWEGSSDINESLAQT
                     VTSSVSYRQPLLSSYLNIGSTYTEDLINNRASISLPSATFSLPSRPVYEIFYHPERSP
                     DAWWSNISYNYNVRFDHIGIMNAPHRDFQDYIWNNETDPENPDFYLTQHNAGIKQQLG
                     LSYNWKALGWLNFQHGLSYNEAWFDRDKNGNNWVRGNDYYAYTNTNFNVYGVRNFQQG
                     WLKSIRHLLTPSAGISFSPDFTKNKQFYNFGGIYLSNAGKAANLNLSLDQRWQIKYGN
                     DNKKINDLIGWRTGISANLVKKDHPFGNLSHSAYFRPGSINLGTFQMPGTKYKLDKIS
                     LAYSAQYSLYHNPYKMHLLDWQPSNQYFSQALTLTGSAPYQKYFTREKNRTFDPYQTP
                     DSLQIFAEEITSLSGQDNWKLSLSHDLSATKSMSDPVSQNLRMDSSFKITQNWSISYG
                     NYVNLKTGKMLSQTFHIIRDLHCWKLDFSYSRRNEYWEYRIALFNVVFPDALRFQTHD
                     SGRS"
     gene            complement(4502..5755)
                     /locus_tag="PK015_03035"
     CDS             complement(4502..5755)
                     /locus_tag="PK015_03035"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HOL33482.1"
                     /translation="MDNFHLTIYQSYLRVMHRFCQYPELVKQLEKGKFSSKTNVLAIG
                     KTAWKMASLCANILAQKNISYEGFVLTKYGLTYGSIPGITVLEAGHPLPDANSFAHSK
                     TIVNWLKNSSPKDDLIILLSGGTSALFEIPEEGISEQQLLEIYKQLLKSGKNIEEMNK
                     ERSKYSQVKNGKALSFVQAKNIKIFAVSDVPENNPHILGSGPFTPAMIDIAVKNGWNY
                     KYGNKDIRYHIVADNKSFCNNLSDEFKSAGLNVYQDPQFYTYSMNKMVNLLTDILDGS
                     AMLPITIQPFIYIAGGELSVKVTGNGSGGRCSHLVLNMINPLSRFNNSALFCFATDGC
                     DNVVGSGGAWADSYTRKEILDAKISIVRAKKEFDSYTALKAINHILPAPILATNVNDV
                     YVLSCGYRLSNHLANQDEPQNNLFR"
     gene            5968..7464
                     /locus_tag="PK015_03040"
     CDS             5968..7464
                     /locus_tag="PK015_03040"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOL33483.1"
                     /translation="MSERVKETRSKSAIKVMREEYNRLLEESFQNTAEYKKGDVVEAP
                     IIDISDKYIIVNLGGKYDAYADVSEYTDEKGELNYSIGDILKGYIVDSNEQGFVIGKS
                     LTRQYVDKNALMDAYERKIPVAGKVYGITKGGFNVDVLGARAFCPLSQISLHGAEPKE
                     KYIGKTMDFQVIECSDNCRRVVVSHRQFLEAQEQEKKMAALEKLHPQDVVKGKVMRMT
                     NFGAFVDLGDIEGLLHVSEISWQHVARPQDVLKVGQEIEVKILDIKEDKIALSMRALQ
                     DNPFEQFIKENQEGDIVNCRVLRLLDFGAFVELKSGVEGLIPLSEISHKRNISHPREL
                     LKEGDYVEAQILRIDPDNQKISLSMRALQADPWDNIDEIVQIDIPFTGKVESSTNFGV
                     FVTVADGITGLLPRSRLREKDSFKPGDEINLVVTEIDRDNHRLTLDYPDHLPEEGRSQ
                     HKRTNEHTSQRDSSISSRRNGKFRGDEEWRRYAVQKTNPSDDNPFKDL"
     gene            7467..8963
                     /gene="lysS"
                     /locus_tag="PK015_03045"
     CDS             7467..8963
                     /gene="lysS"
                     /locus_tag="PK015_03045"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOL33484.1"
                     /translation="MQSRDNQFIQLRKQKLEKLYTAGIDPYPIKSHRTHKISEVLKGK
                     EEWVASGQEVILAGRLVAMRRQGKLGFGDLEDASGKIQIYVAQNLVGEENYELFKLCD
                     SGDFIQAKGTLFFTQAGEYSLKASQISLLAKNLRPLPPIKEKVIDGKLVRYSALSDVE
                     LRYRKRYLDLLLNPSHRDIFIRRAKIISAIRSFLDEREFIEVETPILQPLYGGANARP
                     FVTHHNALNIDLYLRIATELYLKRLIVGGFEKVYELGKDFRNEGMDRTHNPEFTMLEL
                     YEAYSDLEGMMELTESLLQCLAMDVLHQSIYRFKGYDIDLSKPFQRASMQELLKEYLN
                     LELSEDDYEQALAFCKQHDIEVPPGSKTGKLIYLIYEDMVEDKIIQPTFVTDFPKEVS
                     PLAKAKPDNPFWADRFELVVAGYELANAFSELNDPLEQRVRLEAQAALRALGDEEAAV
                     VDEDFLEALEYGMPPMGGLGIGIDRLVMLLTENDSIKEVILFPQMKPE"
     gene            complement(9016..10233)
                     /locus_tag="PK015_03050"
     CDS             complement(9016..10233)
                     /locus_tag="PK015_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOL33485.1"
                     /translation="MDIISIMKIAFKSLARNKTRTFLTMLGIIIGVGAVITMIAIGQG
                     ATKVVNDQISSMGTNVIMVISNTSNTQSRARQAAGSGNLLDEGDVKAIQENIDGVLYV
                     SPVYSTYGQLKYGSNNWRGSIAGVDVDYFLIRDLSLEEGQYFYPSEVESGAKVCVIGK
                     TVADNLFLGEDPVGKTMRIRHIPFTVKGVLKSKGASSMGMDQDDIVIAPYTTTVSRLI
                     GQRWRFMTIMVSAVSKEKIPMVQEDIMNLLLTRHNGTTRDDFIVRSQTDIAEAANTVS
                     KTLTVLLASIAGISLLVGGIGIMNIMLVSVTERIKEIGIRMAVGASKRDVLLQFIIEA
                     ITISLIGGLIGIIMGWSLAVVLGQTMNWSISVTPWSILLAVGFSCAVGIFFGWYPARK
                     AANLNLIDALRYE"
     gene            complement(10234..10995)
                     /locus_tag="PK015_03055"
     CDS             complement(10234..10995)
                     /locus_tag="PK015_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOL33486.1"
                     /translation="MDNNILISVKGLTKTYQMGEVYVHALRGIDLDIDNREFVAIMGA
                     SGSGKTTFMNLLGCLDRPTTGTYLLDGINVADMDDKAITQVRNQKIGYVFQAFYLLPR
                     TTALENVELPLLYCRKMSTKERKEKAMYALEVVGLADRAKHYPNQLSGGQQQRVAIAR
                     AIVNDPVFILADEPTGNLDTRTSVEVMELFQKLHSEGKTIVLVTHEFDISRYALRIIT
                     FRDGKVISDVPNNDVHIASADLKKLPRLDIEEEVS"
     gene            complement(10998..12260)
                     /locus_tag="PK015_03060"
     CDS             complement(10998..12260)
                     /locus_tag="PK015_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOL33487.1"
                     /translation="MKKKYLTYIIIAVVVIAVIVVISQVNKKKNMPEWRTETPSIGTI
                     REVVTATGSLNPTMLVEVGTEVSGKIEKLYKDFNDTVKKGELLAKLDTEILAANLESA
                     KADVNKATIVRDDAKMEYENSKTLYEKGMGSQYEMEKKLYTYQQAEQNLATAILKLKT
                     AQKNLQNASITSPINGVVVSREVSEGQTVAATMSSPTLFKIANNLDIMEITAVVDEAD
                     IGKVRVGMPVEFTVDAYPEEQFYGTVNQIRLKSIVEQNVVSYHVIIDTDNPEHKLLPG
                     MTTNVTIIIQSKEDVMRIPVTATRFLPSKEVWELFGLKWEDNLITKARRAAMQAASSQ
                     NSAKTSTMNAGNKNFSNGRPKFSTIWVLKDNVPELMVVQTGISDGAYIEVLSDLDQKA
                     KIITGVNYKNASQITGNSVMGGPRGGRF"
     gene            complement(12271..13521)
                     /locus_tag="PK015_03065"
     CDS             complement(12271..13521)
                     /locus_tag="PK015_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOL33488.1"
                     /translation="MKRFIWLLFLVPFALMAEQYTLDELIDYGLKNSLTIQSSGLTYE
                     SIRSNLTSAKLNLLPEISLDLGVRNDFYHPETPKASDLSSSAGFSISKSLSLNDPYWY
                     NYKYAQLDEKRGALDLQSKISSYAYNVFTAYLDVLSSQKQLLSLNKNLEIQTRVWEQT
                     KILNQLGKNTSFDVKESEIAVMNSRISIMQLENTIATKRRELFGLVNMKDEGYDLADL
                     EPDKELTIPDFSPETSYAIKTLQNELSRTQLSLKQNKTDFLPQLKLTYSFARNARGED
                     FSFESYNTEHTLSLGISYSIWNQFKQNQTVKRSNLSVRMAELNIQDQLDEMERLYEIL
                     TRELEYLNRLDELYQEKLEQANSQIQIAEERYRLGLIDLLELDKTRVDFINSEISYYS
                     NRYQILAKQEAINNLLSLKIMGKW"
     gene            13746..14747
                     /gene="argF"
                     /locus_tag="PK015_03070"
     CDS             13746..14747
                     /gene="argF"
                     /locus_tag="PK015_03070"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005318430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOL33489.1"
                     /translation="MPVNLKNRHFLTLMDFTPQEIEFLLDLSLQLKKAKYAGTEQPRL
                     KGKNIALIFEKDSTRTRCAFEVAAYDQGAKVTYLGPTGSQMGKKESIADTARVLGRMY
                     DGIEYRGFGQEIVEELAKYAGVPVWNGLTDQDHPTQVLADLLTAQEHLHKPFTDMVFV
                     YVGDGRNNMANALMIGCSKMGMDFRIVSPQSLFPDLSLVDKCKEMANTTGVKITLTDD
                     IDEGVKDADVIYTDVWVSMGEPETVWESRIQLLRPYQVNSELMKKTGKDSTIFMHCLP
                     SFHDLKTQIGKQIGERFNLSAMEVTDEVFESPQSVVFDEAENRMHTIKAVMVATIGN"
     gene            complement(14812..15060)
                     /locus_tag="PK015_03075"
     CDS             complement(14812..15060)
                     /locus_tag="PK015_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33490.1"
                     /translation="MTYKLMLIKIDHRSTEAVKVQSVLTKYGCNIKVRLGLHEVSNDF
                     CSNDGLIVLEVEGEPQVLNDMLQELNSIDYVKAQLIEV"
     gene            complement(15122..16822)
                     /locus_tag="PK015_03080"
     CDS             complement(15122..16822)
                     /locus_tag="PK015_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOL33491.1"
                     /translation="MPDKKNEKNYPSNFIRTIIENDLASGKCKKVVTRFPPEPNGYLH
                     IGHAKSICLNFGLARDYGGVCHLRFDDTNPTKEDVEYVESIMEDVHWLGFDWQDKLFY
                     ASDYFDQLYEYAEMLIKDGKAYVCDLSQEEIRATRGTLTVPGTESPYRNRSVEENLDL
                     FRRMKAGEFEEGSRCLRAKIDMASPNVNMRDPVIYRIRKAEHHRTGNKWVIYPMYDYT
                     HCISDAIEGITHSLCTLEFEDHRPLYDWFLDQLPVPCHPQQIEFARLNLSYTVMSKRK
                     LLELVQSGTVRGWDDPRMPTLAGMRRRGFTPEAIRDFVDRIGVAKADSLVDFELLQFC
                     VREDLNKKANRVMAVLHPLKVTIENYPEDQVEEFEAINNPEDESAGTRMVPFCKYLYI
                     EQDDFSENPPKGWHRLSPGAEVRLKHAYYITCKEVIKDESGNVTELICTYDPSSRGGW
                     TPDARKVKGTIHWVSARHSFDAEIRLYENLFTIPNINDIEEGKDYRDYLNPNSIKVLK
                     NCKLEPSLKEVTIGEKFQFLRQGYFSVDYDSTADNLVFNLIVTLKDSWTKQLKKMGYV
                     "
     gene            17193..18653
                     /gene="guaB"
                     /locus_tag="PK015_03085"
     CDS             17193..18653
                     /gene="guaB"
                     /locus_tag="PK015_03085"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOL33492.1"
                     /translation="MKANIRKTYTFDDVLLVPQSSNILPSSVSLETKITKTIQLKIPI
                     LSAAMDTVTEYEMAIAMAREGGIGIIHKNLSIEEQAKQVQLVKRAESGVITNPYTLSP
                     NDRLTKVWELRAQYKVGGFPVVENGKLVGILTSRDIRFETDGKKLVKDLMTPREKLIT
                     ATVGTSSEECIALLQKHRIEKLILVNPDFSLAGMLTVRDLLKKINYPNAVQDEKNRLL
                     VGAAIGVTGDYLERAQELVHQGANLLVLDTAHAHHSNIRSALNKLKANLNIEIMAGNI
                     ATAKAAEYLIDSGADAIKIGIGPGSICTTRVIAGIGVPQLSAIMDCAEVAEKAGIPLI
                     ADGGIKYSGDIVKALAGGANAVMIGSLLAGTDESPGEAIIYNGRRFKSYRGMGSIGAM
                     KKGSKDRYFQEELEENKLVAEGIEGMVPYKGPVREFLYQLTGGIKAGMGYCGAENLEA
                     LRKKAEFIEITSAGLRESHPHDVNITKESPNYQISE"
     gene            18607..19548
                     /locus_tag="PK015_03090"
     CDS             18607..19548
                     /locus_tag="PK015_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HOL33493.1"
                     /translation="MSTSPKNRQTIRLANDDKLNAELISHLNSFVYYLTVEKGMAENS
                     VESYRRDVRDFLEYNPQPLSEYTAQDITGYLTDLMEAGLVNTSVARKRVALKQFFTFL
                     EDNGENIQVDFAQVPKIKIGQHLPDYLDVETMLKLLDGLPTSTPLEKRNKVMLELLYA
                     TGMRISELLNLTIHDINFSEQVILVRGKGSKQRFVPFVDSVAVLLQEYIKDVRPVLLK
                     FEHTDILFLNNRSQKLSRMGFWKILRKITQEAGLKQEITPHTFRHSFATHLLEAGVNL
                     RIVQALLGHSSLNTTQIYTHIDTRYLVETHRMYHPRA"
     assembly_gap    19978..20077
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(20103..21605)
                     /locus_tag="PK015_03095"
     CDS             complement(20103..21605)
                     /locus_tag="PK015_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33494.1"
                     /translation="MKKSLFLLLPCLILLIGIIPQVLVAQTQKLPPVEISSEVAPKAP
                     LIKKPLNFNFEIPQDSIPPYLPRVYTAKYMPPAITDKEMKPLSLNIGMKSEFSSYLYA
                     ALFTNNSQLPFIDFDGNFSVPGTNRSLKNLQLRALSDFHPLPEFAHNLQYQEASSDSF
                     SSSLISYTLFAPAKNLTKGKISLNQVQTWINVENLNQKDNQDSVDDFSFGIRHSNDFS
                     FKDNTFSNLFLFQDSAWGIKAQYHINLTPEKYPALEAGLMTDFNHLLPSINLHKRWIT
                     GPGKQLELENKAELTSFSLANLKSNSPWTIVPEKDYICMSPLNLSNFYWINFKPENRL
                     WNKIGFGQNIKFSYNVPSIYSNPVSGQTYYRFEEVFSYQPQMEFSFYVKDLNCYQNLK
                     LNMEFLPDKNWQRKSYSPLLTANTSIKSKYKNLNLITTLEQEYFRYDEDHNYLPFIFD
                     LSLEAQYPIEENLLLSLQLQNLFNTPYQLPGNLPSSGRTVFLLLKYRPFS"
     gene            complement(21608..24403)
                     /locus_tag="PK015_03100"
     CDS             complement(21608..24403)
                     /locus_tag="PK015_03100"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOL33495.1"
                     /translation="MFYNSDGYLDEAAKEIAVFQATYPDSPYLLYLDYLSANIALKQG
                     DFHKSSLLYKKIINQNLPPDIVNDIYLNYAICCYYRDDYKTGIQLLNSLEKSAIHPWY
                     ISQAHLWKGRIYSREELWLSAEQELHKALASGEKSAFSDYFQTLVYLERDSTVTALLD
                     SLSAELPETIDYFGIWLEHLLNSGRYKEFDEYLTRLSPYTTASTQIGLLQVRKALNQE
                     DLVQATALLDSLNIHNDLANYYRAIVYLKTGNISASDSLFQTLIKSSDSNLACLSYLE
                     RLKILAHKDKNLAFQQLDKFIKEKRPQCGEAYQLLGKWQLEKGNYKDAIHNFLAAFNY
                     PMEPLSRELNYLFCAETWYYLNNYKSCAETCNNYLRKYPSGKFCDSIIYYLAESALMT
                     GDTEKAKSYYTELIQKYPHSKWIDIARFKLAEIYYQASNYKAAEELYKNITPSPENYS
                     ILYLRLAQTYYYQDKYDSAKQILEQVLEPAKNFEASILLASIHFNQKDYEQALKLYRQ
                     AENLATSKVQENEAKAYSAYTLFYLKRYNEATNLFLELAQDSPNAEIFLLQAAKSAAQ
                     GKNWNRALQLYDNFLDKYPESDHYLQALSDLANIYYNLGEYEEALDYWMGVIRNFTSN
                     TFLTDEELIFLSDVFTGIELSARKLRDTDKIDELADMIDLFHSEYIKFELEYILVKLY
                     AESELWNELLREANKFKTSLNLPQKQISDIDLLMLEALINLNRLSEAESLAQQIYEAS
                     PSQEVLIKWAELAELNGNIQLALERYREAFALKPDADTWLKMLELSYKDDYKDFMEIW
                     KAGTPFINDNPQAYIVYLQYLWEKVSPDATFALADSLLQTQTEPYVRAICDFYQGRTY
                     YQKEDYPSALRSFRKIRLLYKDYPELYRDASYFYILTLINLNELQEAQLCLQEEQNIL
                     LKEQINHLQQLLPTEKQ"
     gene            complement(24559..30009)
                     /locus_tag="PK015_03105"
     CDS             complement(24559..30009)
                     /locus_tag="PK015_03105"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOL33496.1"
                     /translation="MFIDHRYEVLESLGRGRWANVYKVRDIRNDNIYTLKLFQYLSSE
                     EFYRYFKPKDMHHITKIEHPNLSKMVDFGNVGDHIYSLSVFFDGITLNSFHFNKSKVN
                     ALYDIIVQICYALDALHTQNILHRDLKLENVLYRTSNNQIEVKVIDYGFSRWDLDKES
                     QTVSGTLPYIAPEIYQGKPATPASDFYSLGVILYKIITGNFPFTLEQINAIRSGSRQY
                     FIPIFPSELNPHIPLHLEKLCLRMLERNPENRFQSSAEIISYINRTLGKKYPFSESWS
                     LINSIKFNSYTVQEKIVNQLLDYLPQLEHSNGKIISLVGGEGIGKASILSLFRYNILK
                     GSYFIFDYTCTRTDHEAFFALIKEYLQSLSPEEIERYKSMPTMTEQLRRYVFMSEQAA
                     KEITPTKDELRADFEFCRDVLIELAQRKPIIFIVRDIQYLHHNTIDFINYLSPTVAQY
                     PILIVMSSTDFNKVKQIKHTVLISVPMFTPDEATAYIHKLLSPEVSQNFCDIIYERSA
                     GNPYFIREILIDLALKKQIRYEGKLIYPENLDNYVLPSRLLHSIYYRMSHLTEVNYKH
                     LQKLSVVQTPISIELIRYICKVKDEELYNLLNESKYNEILEKRGEYYYFTFREAKERF
                     FEECDFRIHKLVSLRVLKYYEKKKVNDISTCEGIIHNAQIAQDKVGERKWLLNLFQLL
                     KENYLQEEAQKTIIQVLQIDLNPDMELADEELLTDLAETHKMIEITGNIQGLDFIEEA
                     TNNIPDSYEKYLLLGTIKLLSDNTTNAYRYFIKAEKLARTPTQKVTAILHQAQCYMEK
                     DPFRMKKFLDRAANLEMDLALKITYTNLFALYLFHRSQRDLAISTIEDFLAQLPPDQD
                     LEVMINLALLHNELGEFYSLQKNVIEAEEHFNQALNIWNKYRVDRYLTRIHNNIADLH
                     LKQGFTVSGLNHSQKALDFAIKRGDHLAQGRALLNQGEAMIKMGKFEEAEAKLMEASQ
                     ILSSLKTKKYEDVVIRNLALAKSKIKGFGHYYQFISKNEPRLIEGVIQEINPLVKTYL
                     YYLSEISYPSKLKKLLQKNAHIDYELIQEQEFYHNVLSLLAISEKDYHKALEELKLAS
                     QFAGDINNHYAMAVFNVLRAKCYYGLGEYVKAQEIINNARPTIQEYQYKYWEKSLDIL
                     ELKLKLADASIPLRSILRKTIQILEECQQSQYYQLIVELWQIKIQILVELGALKQAEK
                     EFQDYKQYLHKITSDISPDDRENFLRKNLFGLKNVKYFNAVMVASRKRDIRNQVNDLI
                     FTISNVTSVQQMKFLVEKGITQLLSPWQFKLMVFSEKMNSYYAFLSYNWDPDMMLPAE
                     FSPYIEQSFTSDNLVRFRYQQHNILIIPLLSGTRRIGFLVLSDNGELPYTQGELTIAR
                     SLKTHLAALIIRINDYEEITLRMEKMNQLINISRDLLGILEMNELEAGIVSAAIDLTG
                     STRGFFISKDEEGNNLYKVQMDQNKQPLTSTAGISNTALSLCQTNQEPIMAYNAQLDN
                     RFKNAISVQDYAIQTIFCCPIIVDNLNYGYLYLDNYGDSSREMYLNDDIIKLFLDQVA
                     NAIRNAQKYAELLEKSNELSNLEQSKDEFVEIVAHELNTPLTAIQGYVSRLKRKLYSD
                     EEERQEIISKLEAAVNKLATSIGDITTMNLYNITQSLPKSPIKIEEIIDLVQQEVLIL
                     SRQRKMQIKVEIEEGLPPVNGNWEALHRMIYNVVLNAVRFTREFGTIVIGARKSAFPQ
                     ERINNQDSLVIYVKDNGIGIPEYQLKNVYRKFYELNDIYAHKSGTVEYRSSGLGLGLA
                     ISKRIAELHNGQIIIKSKENEGTSVFMIIPFK"
BASE COUNT         9278 a         5374 c         5656 g         9718 t
ORIGIN      
        1 tcagcatttg ccgggttttc tctgctttgc gtttacttcc tatcatacct aaataacgaa
       61 aaggcttatc taaacattgt tccagcacct gttcatcgta gatatgcccc tgagtcataa
      121 tgacaataag ggcattttga ttgaattgaa tgtgcttttc caagtcctta taatcgctta
      181 aatggaggtc attacagatt tctggaggaa tattctccag agcttcagga cgattatcaa
      241 tgattctggt ctgaaaatcg cagagattag ctaaatgagc taaggctttt ccacaatgtc
      301 ctgcaccgat gatgtaaaga atgtttgaag agaaaagtgg ttctataaag acagaaacct
      361 tgcctccaca gaccattgat aagggaatac cttctgttcg ttctggtttt tcaccttctt
      421 cagaaagcag ataagtttcc agaattgggg aagaaggttg ttccttacga acccacgata
      481 tgagcaagtg ttccagttct ccaccaccta aatttccata cacctttccc gacaaaggga
      541 taagcatttt cattcctggt tttgcaggag tggaaccaat agaagatata atagtaactt
      601 gccagacagt ttgattttta gatattagtt catttaagat agcataatat tctttatttt
      661 cggacataat tttccttcct catttttgcg gtaagtttcc ccaaactatc tgataggcac
      721 aaattgcaca agcaacagca acattgagag aatttttcca gccttgaacc ggaatagcga
      781 gaaaactatc acacagttta agagcatctt ggcttatacc cagactttca tttccgacta
      841 ctaacgccag aggaaattga taattaactt cataaatgga cttcgctttc gctgcagttt
      901 ctaaagcata aatataataa ccttctgatt tagctgaaag aatagcagct tccgtttgag
      961 ggaaatattt ccactgcact ttatcctccg ttcccatagc agtctttttt aaagcagcac
     1021 tttctggtgt ggcacaaact ccacaaagat aaatggcttg aagatttaaa cattcagcgg
     1081 tgcgaaaaat tgaaccaata ttgaatgcgg aacgaagatt atccagtatc aaaattattt
     1141 gctgagcttt tctttgggct tccgcatcaa ccacttttaa gccatctcct tttctaactt
     1201 gaatctggta atctttaaag gtttttcctc gtgctctctg gtattggact aaagcattat
     1261 agattttatc aaaggattct tcttgttgca aatcctgact tagctgggaa acacattccg
     1321 gtaatggtga ctgcatccag gaaatacaat gtattatatg ctcttttaat tcaaagtaat
     1381 tattgttttg agaaagagat tgcgaaagag aggaaactaa cttatccaga gccttcaatt
     1441 gatgaacagg agaaagtgaa gaaaatttgc tctggctaaa atgatttatg tttctcattt
     1501 tgttagcatt tgaataagct tagcagcagt gagtaaattt tctgctatga aatcaggtgt
     1561 atattcccag ccttcaaatc cagtcagaag ttcttctttt ccgtgaccac tcagaagtag
     1621 caccgttttt aatcctgctt ttttgccaaa gtaaacatcg gactttctat ctcccagcat
     1681 ccaggattta ttcgggtcaa aagggtgatc tctcatagct ttatagaaca tacctaatcc
     1741 tggtttgcgg tcttcatgag caatattgaa aggaggcact gttcctgttt ccagataagg
     1801 agaataatat atatcagtaa gaattatacc ttcattattt agcaattctc tgagtttggc
     1861 gtgaaccttt tctaatgttt ccaggtcaaa atatccttta gcaatcccag cttgattagt
     1921 gactatgatt agcatatagc ctaaattctg caaaatccgg agtgcttcaa tagtataagg
     1981 ataaagatgg tatttttccg gatctggttt tagtccaaaa ttatccgggc taatgcaacc
     2041 atcccggtca agaaatacag cttttttaac ttcttccact atcatgagtc tggaatctta
     2101 aagcatcagg gaacactaca ttgaataatg ctatccggta ttcccagtat tcatttcttc
     2161 tactgtaact gaaatctaac ttccagcaat gtaaatcgcg gattatatga aaagtttgag
     2221 agagcatctt gcccgttttt agattaacat aatttccata actgatagac caattttggg
     2281 taattttgaa agaagaatcc attctgagat tctgtgaaac gggatcggac atacttttag
     2341 tggcactaag gtcgtgcgat aacgataatt tccagttatc ctgtccactt aaagaggtga
     2401 tttcttcggc aaatatttga agactatcag gtgtttgata agggtcaaag gttctattct
     2461 tttctctggt gaaatatttt tgataaggag cagaacctgt aagagttaaa gcttgagaaa
     2521 aatactggtt ggaaggttgc caatccagaa gatgcatctt ataaggatta tgatagagac
     2581 tatattgggc tgaataagca agagagattt tatccagttt atatttagtt cctggcattt
     2641 gaaaagttcc cagattaata gaccctggtc ggaaatatgc gctgtgggat aaattgccaa
     2701 aaggatggtc ttttttcacg agattagcag aaattccggt tctccatcca attaagtcat
     2761 taatcttttt gttatcattt ccatatttaa tctgccatct ctggtctaaa gagagattca
     2821 gatttgctgc cttaccagca ttacttagat atattccacc aaagttatag aactgtttat
     2881 ttttagtaaa atctggactg aaactgatac cagcagaggg tgttaataaa tgacggatgg
     2941 attttaacca tccctgctga aagtttcgca caccataaac attgaaattg gtattggtat
     3001 aggcataata atcatttcca cgcacccaat tattaccatt tttatcccga tcaaaccagg
     3061 cttcattata ggataagcca tgctggaagt ttaaccatcc taaggctttc caattataag
     3121 atagaccaag ttgctgcttt atacctgcgt tatgctgagt tagatagaaa tctggatttt
     3181 ctgggtcggt ctcattattc cagatataat cctgaaaatc tctatgaggt gcattcatta
     3241 tgcctatatg gtcaaagcga acattatagt tataactgat attactccac caggcatcag
     3301 gacttcgctc tggatgatag aagatttcat agactggtct ggaaggcaaa gaaaaagttg
     3361 cagaaggaag tgaaatactg gcacggttat taattaaatc ttctgtgtag gttgaaccga
     3421 tatttagata ggaactaagt aagggttgac gataggaaac agaagaggtc acagtttgag
     3481 cgagactttc attaatatcc gagctacctt cccagatacg tttattgctg acaaaatcta
     3541 catttaaatc aaaagtagct ttatttccga gctcttgatg atgatttgct ctaaccgacc
     3601 aatcattgta ggtttgtata tcagtaagct ttttctgata ggcggcattc agatttccat
     3661 ttaagatata acgcttgagg taactcaagt ttaaatccgc tttccatccc gtcttttcat
     3721 aaaggtcaaa actcacaatg atatctgcat aatctttata aggatagtac caggcaatat
     3781 ctttaaggaa tttgccatct accacattat aaccagggac aggaattaaa aaaccaggat
     3841 gccttcctcg ctctaaagga atagttatga acgggaaata gaaaataggt agatgattta
     3901 cataagccac tacatttttc cctacaattt tatctttatt atagatgcgc acctgttttg
     3961 ccgtaaacca gaaatcaggc tcctcatatt cgcaggtggt gaaattgcaa tcgtctacat
     4021 cataagtctc aacatctatt ttccgcacct gtttcccact ataaaaactc ttgtccatac
     4081 tgctgatacc attctccatt ataccggttt gagtttctat atcataagcc acatttgaac
     4141 ccagaaggat ttgagcccca tcctgcataa cagtattacc tacactataa gcacgattgg
     4201 tctttaaatt tataacaata gaatcactgc taatggtgaa ttcttgataa cggatattgg
     4261 tattgccata gagccttatc tgttcaagat tttggtagta ggacacacta tccgctgaat
     4321 agaaaagcga atcttcttta actttagctt gagtgagagt atctggcaca acttcaggta
     4381 gttttgaggg aaaaacatct ctctcatttt gtgccagaag tactaaagat agggggaaag
     4441 ataaaattaa caggatgagt ataaacttat tcatcagaag ctaaaatcag tcagtttgcc
     4501 ttcagcgaaa taggttattt tggggttcat cttgattggc taaatgatta gataatctat
     4561 aaccacaaga aaggacatac acatcattga cattagtggc taaaattgga gcaggaagta
     4621 tatgatttat agcttttaaa gcagtgtagc tatcaaattc cttttttgct ctgacgatag
     4681 aaattttggc atcaagaatt tctttacggg tgtagctatc tgcccaagca cctccacttc
     4741 cgacaacatt atcacaacca tctgtagcaa aacaaaatag cgcagaatta ttaaacctgg
     4801 atagaggatt aatcatattt aaaaccagat gactgcaacg acctcctgaa ccattaccag
     4861 ttactttaac agaaagctca ccacctgcaa tatagataaa aggttgtata gtaataggta
     4921 gcatagctga gccatctaat atatcggtta ataaattaac cattttattc attgaataag
     4981 tgtaaaattg ggggtcttga tacacattaa gcccggcaga tttaaattca tcagataaat
     5041 tattacaaaa ggatttgtta tctgccacga tatgatagcg gatatccttg tttccatatt
     5101 tatagttcca gccatttttc actgcaatat ctatcatagc tggtgtaaaa ggtccagagc
     5161 ctaaaatatg aggattgttt tcaggaacat ccgagaccgc aaagatttta atatttttag
     5221 cttggacaaa agaaagcgcc tttccatttt tcacctgaga atatttagac cgctctttat
     5281 tcatttcttc tatatttttt ccgcttttta agagttgttt atatatctca agcagctgtt
     5341 gttcgctgat tccctcttcg ggaatttcaa acaaagcaga agtgccacct gaaagtaata
     5401 tgataaggtc atctttaggt gaagaatttt ttagccagtt tacaatggtc ttgctatggg
     5461 caaaactatt agcatcagga agtggatgtc ccgcttccag cacagttatc ccaggaatag
     5521 aaccgtaagt taaaccatat ttggttaaaa caaaaccctc ataagatata tttttctggg
     5581 caagtatatt agcacaaagc gatgccatct tccaagctgt ttttccaatc gccagtacat
     5641 tagttttaga actgaacttt cccttttcca gctgcttcac aagttcagga tattgacaaa
     5701 acctatgcat cactctaaga tagctttgat agatggtaag gtggaaattg tccaaattta
     5761 agctcctgaa ttttattaat ttattccctt ttctataaat aatcaattag tcaagcggat
     5821 ttttgagttt gatataggta agagctgatg tttaagggtt gatatttatc ttaaagagga
     5881 agaaatacat aagaacaaca aaaaaacctt tacaaaaaca ctctctctga aaagttgata
     5941 ttcttgaatt gtcctgagga gtcaattatg tcagaaagag taaaagaaac acgcagtaaa
     6001 tctgcaatta aggttatgcg ggaagaatat aaccgcttac tggaagagtc cttccagaac
     6061 accgctgaat ataaaaaagg ggatgttgtt gaagctccca taatagatat cagcgataaa
     6121 tatataatag ttaatctggg cggcaagtat gatgcttatg ccgatgtaag tgaatatact
     6181 gatgaaaaag gcgaattgaa ttattctatc ggtgatattc taaaagggta tatcgttgat
     6241 tctaatgagc aaggttttgt cattggtaag agtttaactc gtcaatatgt agataaaaat
     6301 gccctaatgg atgcttacga gcgtaaaatc cccgtagcgg gtaaggttta tgggataact
     6361 aaaggtggat ttaatgttga tgtgcttggt gcaagagcat tttgtccctt atcccagata
     6421 tccctacacg gtgcagaacc taaagagaaa tacattggca aaacaatgga ttttcaggtt
     6481 atagaatgct cggataattg tcgtagagtt gtggtatctc atcgtcaatt tcttgaggct
     6541 caggaacagg aaaagaaaat ggctgctttg gaaaagcttc atcctcaaga tgtagttaag
     6601 ggtaaagtta tgcggatgac gaattttggt gcctttgtgg atttaggaga tatagaagga
     6661 ttacttcatg tatccgagat ttcctggcaa catgtagcca gacctcagga tgtgctaaaa
     6721 gttggacagg aaatagaagt aaagatttta gatatcaaag aagataaaat agccctttca
     6781 atgcgagcct tacaggataa tccttttgaa caatttatta aggaaaatca ggaaggcgat
     6841 attgtcaatt gccgtgttct tcggttactg gattttggag cttttgtgga actcaaatcc
     6901 ggagttgaag gtcttattcc tctatccgaa ataagtcata aacgtaatat atcgcatcca
     6961 cgagaattat taaaagaagg tgactatgtg gaagctcaga ttttgaggat agacccagat
     7021 aaccagaaga tatcgctttc catgcgtgca cttcaagctg atccctggga taatatagat
     7081 gagattgtgc aaatagatat accctttact ggcaaggtag aaagctctac caattttggt
     7141 gtgtttgtta ccgtagctga tggtattacc ggtcttttac ctcgttctcg tttgagagag
     7201 aaagattctt ttaaacctgg tgatgaaatc aatcttgtag ttacggaaat agatagggat
     7261 aatcatcgtt tgacccttga ttatccagac catttacctg aagaaggtag aagtcagcat
     7321 aaaagaacta atgagcatac cagtcaacga gatagctcta tatcatcccg gagaaatggt
     7381 aaattccgtg gtgatgaaga atggcgtaga tatgcagttc aaaagactaa tccctcggat
     7441 gataatccct tcaaagactt ataataatgc aatcccggga taaccaattt atccagcttc
     7501 gtaaacaaaa actggagaaa ttatatactg caggaattga cccttatcct ataaaatcac
     7561 atagaacgca taaaatcagt gaagttctaa agggaaaaga ggaatgggtc gcttccggtc
     7621 aagaagtgat attggctgga agacttgtag ctatgcgtcg tcagggcaaa cttggttttg
     7681 gtgacctgga agatgccagc gggaaaattc agatttatgt ggcacagaac cttgtaggcg
     7741 aagaaaacta cgaacttttc aagctttgtg attcaggcga ctttattcag gcgaaaggaa
     7801 cgctgttttt tactcaggca ggtgaatatt ctctcaaagc cagccagata agtttattag
     7861 ccaagaatct tagaccctta cctcctataa aagaaaaggt catagatggc aaattagtgc
     7921 gatattcagc tttatctgat gtggaacttc gttatcgcaa acgctatctg gatttgttat
     7981 taaatccatc acatcgggac atttttattc ggcgagctaa aatcatttca gctattcgct
     8041 cttttctgga tgaacgagag tttattgaag ttgaaactcc tattcttcaa cctctttatg
     8101 gaggagcaaa tgctcgtccc ttcgtcactc atcataatgc cttaaatatt gacttatatc
     8161 ttagaattgc tacggagtta tatctaaaaa gacttattgt aggtggattt gaaaaggttt
     8221 atgaattggg taaagatttt cgtaatgaag gaatggaccg cactcacaat ccagaattta
     8281 ctatgttgga actttacgag gcttattctg atttagaagg tatgatggag ctgacagaat
     8341 ctttattaca atgtttagca atggatgtgc ttcatcagtc tatttatagg ttcaaaggat
     8401 atgatataga tttatccaag ccttttcaaa gagcttcaat gcaggaactt ctgaaggaat
     8461 atctgaactt ggaactttct gaagatgatt atgagcaggc tttggctttt tgtaaacagc
     8521 acgatattga ggttccacct ggttctaaaa cgggtaaact tatatattta atctatgaag
     8581 atatggtaga agataaaatt atccagccaa cattcgtaac tgattttccc aaggaggtct
     8641 cacctctggc aaaagctaaa ccagataatc ctttctgggc tgatcgcttt gaattagttg
     8701 ttgccggata tgagcttgcc aatgctttca gtgaacttaa tgaccctctg gaacaaagag
     8761 ttagacttga agcacaagct gcactaagag cgttgggtga tgaagaagct gctgtagtag
     8821 atgaagactt tctggaggct ctggaatatg gaatgcctcc tatgggtggt ttaggaatcg
     8881 gtattgaccg tttagttatg ctgctcactg aaaatgattc tatcaaagag gttattctgt
     8941 ttcctcagat gaaacccgaa taacctcatt caatagaatc catctcaatt cttatatact
     9001 ttaattctct tttccttact catacctgag ggcatcaatc agattcagat tagctgcttt
     9061 acgagcagga taccaaccaa agaatattcc aaccgcacag gagaaaccta ctgccagcaa
     9121 gatagaccat ggcgtcacag atatactcca attcatagtt tgaccaagta ccacagccag
     9181 agaccagccc ataataattc caattaaacc gccaatcaag ctaatagtga tagcttcaat
     9241 aatgaactgg aggagtacat ctcttttact tgctcctact gccatccgga ttccaatttc
     9301 cttgatacgt tctgttacag agaccagcat aatgttcata atcccaattc caccgaccaa
     9361 aagtgatatt cctgcaatac ttgccagaag gactgttaat gttttagaga cagtatttgc
     9421 tgcttcggca atatcggttt gagaacgaac gataaaatca tctcgggtag ttccattatg
     9481 acgagtaagc agcagattca ttatatcttc ctgaaccata gggatttttt ctttagagac
     9541 agctgagacc attatagtca taaatctcca gcgctgtcca atcaatctac taacggtggt
     9601 agtataggga gcaatcacta tatcatcctg gtccattccc atagaacttg cacctttaga
     9661 tttaagaaca cctttaacag tgaaaggaat gtgacggata cgcatcgttt taccaaccgg
     9721 gtcttcacca agaaagagat tatcagctac cgttttgcct ataacacata ccttagctcc
     9781 actttccact tctgaaggat agaagtattg accttcttcc agagaaagat cacggataag
     9841 gaaataatca acatctactc ctgcaatact tcctctccag ttattactac catactttaa
     9901 ttgaccgtag gtactataaa ctggagatac ataaagcact ccatcaatat tttcctgtat
     9961 agctttcaca tcgccttcat ccagtagatt tcctgaacct gcagcttgtc ttgctctgct
    10021 ttgagtattg ctcgtattgg atataaccat gatgacatta gtgcccatac tactaatttg
    10081 gtcattgacg accttagtag ctccttgacc tatagcaatc atagttataa ctgctccaac
    10141 accaataatt atgccaagca tagtcagaaa ggttctggtt ttattccgag caagagattt
    10201 gaaggcaatc ttcataatgc ttataatatc catttaagag acctcctctt ctatatccag
    10261 acggggaagt tttttcaaat ctgctgaagc aatatgaaca tcgttattgg gcacatcgga
    10321 gattactttt ccatcacgga aggttataat cctgagtgca tatcgtgata tatcaaattc
    10381 atgagttacc aggacaatag ttttaccttc tgaatggagt ttctgaaaaa gctccataac
    10441 ctctacactg gttcgggtat caagattccc agttggttca tcagccagaa taaagactgg
    10501 gtcattaaca atggctctcg ctattgccac tctttgctgt tgaccaccag aaagctgatt
    10561 gggatagtgt ttagcacggt ctgctaatcc aacaacttcc agtgcataca tagctttttc
    10621 tttgcgttct ttagtagaca ttttacgaca atagagcaaa ggaagctcaa cattttccag
    10681 tgcagtggtt cttggtagaa gataaaatgc ctgaaacaca tatccaatct tctggtttct
    10741 cacttgcgtt atagccttat catccatatc tgcaacattt ataccatcca gtagataagt
    10801 gcctgtagta ggtctatcaa gacagcctaa tagattcata aatgtagtct ttcctgaacc
    10861 gctggctccc attatggcaa cgaattctct attatctata tccagatcta tacctctaag
    10921 tgcatggaca taaacttcac ccatttggta ggtcttggtt aaacctttaa cactgattag
    10981 aatattgttg tccatactta gaatctgcct cccctgggac cgcccataac gctattgcca
    11041 gttatttgag aggcattttt ataattgact ccagtaatta ttttagcctt ctggtcaagg
    11101 tcacttaata cttctatata ggctccatct gaaattccag tttgaactac cataagttct
    11161 ggaacattat ccttgaggac ccagatggtt gaaaatttag gtcttccatt agagaaattc
    11221 ttatttcctg cattcatcgt gcttgtttta gcagaattct gtgaagaagc tgcttgcatt
    11281 gctgctctac gagcctttgt aatgagatta tcctcccact ttaaaccaaa tagttcccac
    11341 acttctttac tgggaagaaa tcgggtggct gtaacgggta tccgcataac atcttcttta
    11401 gattgaataa ttatagtaac attggtggtc ataccaggaa gcaacttgtg ctctggatta
    11461 tctgtatcaa ttataacatg ataactaacc acattttgtt ccacgataga ttttaatcgg
    11521 atttgattga cagtgccata aaattgctct tcaggataag catccaccgt aaattctact
    11581 ggcataccta cccttacttt tcctatatct gcctcatcaa caacagccgt aatttccatt
    11641 atatcaagat tatttgctat tttgaaaaga gtgggagaac tcattgttgc tgctaccgtc
    11701 tgaccttctg aaacttctct ggatactacc accccattta tcggagaagt gatagaagcg
    11761 ttctggagat ttttttgggc agttttaagt ttcagaatag cagttgccag attctgttct
    11821 gcttgttgat aggtataaag tttcttttcc atttcatatt gtgagcccat acccttttcg
    11881 taaagggtct tactgttttc atattccatt ttagcatcat cacgaacgat agtggcttta
    11941 ttgacatctg ctttggctga ttcaagattg gcagctaaaa tttccgtatc cagttttgcc
    12001 agtaattcac cttttttaac cgtgtcattg aaatccttat acaatttctc tatttttcca
    12061 gacacttctg ttcctacttc taccagcata gtaggattta atgaaccagt tgctgttaca
    12121 acttcacgga tagtgccaat agaaggagtt tctgttctcc attctggcat atttttcttc
    12181 ttattaacct gtgatataac cacgattact gcgatgacta caacggcaat aatgatgtaa
    12241 gtgaggtatt tctttttcat atgtccttct ttaccattta cccataattt ttaatgaaag
    12301 tagattattt atagcttctt gcttagctaa aatttgataa cgattagagt aataggaaat
    12361 ttctgaattg atgaaatcca cccgagtctt atccagttcc agtaaatcaa ttaagcccag
    12421 acgatagcgt tcctctgcaa tctgtatttg actattggct tgttccagtt tttcttgata
    12481 tagctcatcc agacggttca ggtattccag ttcacgagtc aatatttcat agaggcgttc
    12541 catctcatca agctggtctt gtatatttaa ttctgccatt ctaacactca gattggaacg
    12601 ttttaccgtc tgattttgtt taaattgatt ccagatagaa tagcttattc ctaaggaaag
    12661 cgtatgttca gtgttgtagg attcaaaaga aaaatcttcg cctctggcat tccgagcaaa
    12721 actataggtg agcttcaatt gaggcaggaa atctgtttta ttttgtttta agcttaactg
    12781 agtgcgactt aactcattct gaagagtttt gatagcatag cttgtctccg gagaaaaatc
    12841 tggaatagtt aattctttat ccggttctaa atcagctaaa tcatatcctt catctttcat
    12901 attaaccaag ccaaatagtt ctcttctctt tgtagcgatg gtgttttcca gttgcattat
    12961 agagatacga gaattcatta ctgctatctc actttctttg acatcaaaac tggtattttt
    13021 ccccagctga tttagtattt tagtctgttc ccagactctg gtctgaatct ctaagttttt
    13081 gttcaatgag agcagttgtt tctgggagct taacacatcc aggtaagcag taaaaacatt
    13141 gtaagcgtag gaactgattt tactttgtaa gtccagagca cccctttttt catccagctg
    13201 agcatattta tagttatacc agtaggggtc attcaagcta agacttttac ttatgctgaa
    13261 gccagcgctg ctacttaaat cggatgcttt tggagtttca ggatggtaaa agtcattacg
    13321 gacgcctaaa tccagagata tttctggcaa aagattgagt tttgcagagg ttaagtttga
    13381 acgaatggat tcataagtta aacctgagct ttgtattgtc agactattct ttaagccgta
    13441 atctattagc tcgtccagag tatattgctc agccattaag gcaaagggca ccagaaagag
    13501 caaccagatg aagcgtttca taattctata ttccttaatt ctaaaatatt tttccgatag
    13561 aaagagaaaa atgctctatt tatttcttac ccataatgta tcaatttcct tcatttttct
    13621 cttcaaatta ttcaataaag cccttatttc tgtaggttaa gaaatagaca aggaaaactt
    13681 cttgccttta aaagtcagtt ttttaaagct gctcttataa ggtaaatgat aaaaaggaga
    13741 ttattatgcc ggtaaattta aagaatcgtc attttcttac cctgatggat tttactccgc
    13801 aggaaattga gttcttatta gacctttcac ttcagctaaa aaaagctaag tatgctggca
    13861 cggaacaacc tcgtcttaag gggaaaaata ttgctcttat ttttgagaaa gacagcacgc
    13921 gcactcgttg tgcctttgaa gtagctgctt atgatcaagg tgctaaggta acctatttgg
    13981 gtccaactgg aagtcagatg gggaaaaagg aatccattgc tgatactgcc cgagttcttg
    14041 gcaggatgta tgatgggata gaatatagag gttttggtca ggagattgtg gaagaactgg
    14101 caaaatatgc tggcgttcca gtctggaatg gtttaactga tcaagatcat cccactcaag
    14161 ttctggcaga tttactaacc gctcaggagc atcttcataa acctttcact gatatggttt
    14221 tcgtctatgt gggtgatgga aggaataata tggcaaatgc cttgatgatt ggctgttcta
    14281 agatgggtat ggattttcgt attgttagcc ctcaatctct ttttcctgat ctctctttag
    14341 tagataaatg taaggaaatg gctaatacta cgggagtaaa aattacttta actgatgata
    14401 ttgatgaagg tgtaaaagat gctgatgtaa tctataccga tgtttgggtc tctatgggag
    14461 agccagaaac cgtatgggaa tcacgcattc agcttttaag accttatcaa gtgaattccg
    14521 aattgatgaa aaaaacaggg aaagacagca ctatctttat gcattgtctt ccttcttttc
    14581 atgatttaaa gactcaaata ggaaaacaga ttggagaaag attcaatctc tctgctatgg
    14641 aagtcactga tgaggttttt gaatcacctc aatcggtggt atttgatgaa gccgaaaata
    14701 gaatgcacac cattaaagct gtgatggtag cgaccatagg aaattaaaga caatataaaa
    14761 actctctctc gcttgcaatt atcacgagag agagtttttc ttcttgatta attaaacttc
    14821 aattaactga gcttttacat agtctataga atttaattcc tgaagcatat cattaaggac
    14881 ttgaggttcc ccttctactt ccagaacaat taatccatca ttagagcaaa agtcattaga
    14941 gacctcatgc aatcctaaac gaaccttgat attgcatcca tatttagtca ggacactctg
    15001 aactttcact gcttcagtgc tacgatggtc aatcttgata agcattagtt tgtaagtcat
    15061 aataacctcc tgaattcctt tttagaaaag attatttaat tgtccataaa aattataata
    15121 ttcagacata tcccatcttc ttaagttgtt tagtccagct atccttaaga gtgacaatga
    15181 gattaaaaac cagattatca gcagtggaat cataatctac agaaaaatat ccctgacgca
    15241 ggaactggaa cttttctcca attgtaactt cttttaacga aggctcaagt ttgcaattct
    15301 ttaagacttt tatggaattg ggattcagat aatctctata atctttacct tcttctatgt
    15361 cattgatatt tggaatagta aagagatttt cataaagtct gatttcagca tcaaaagaat
    15421 gtcttgcact aacccaatga atagtgcctt tcacttttcg tgcatcagga gtccagcctc
    15481 ctctggaact gggatcgtaa gtgcaaatca gttctgtaac atttcctgat tcatctttta
    15541 tcacttcttt gcaagttata tagtaggcat gttttaaacg aacttctgca cctggtgaaa
    15601 gtctatgcca gccctttgga ggattctcac taaaatcatc ctgttcaatg taaagatatt
    15661 tacagaaggg aaccattctt gtgcccgcag attcatcttc tggattattg atagcttcaa
    15721 attcttctac ctggtcttca ggatagtttt caattgtaac tttcaaagga tgtagaacag
    15781 ccatcaccct atttgctttt ttattaaggt cttctctcac gcaaaactgg agaagttcaa
    15841 aatccaccag agaatcagct tttgctactc ctatcctatc tacaaaatcc ctaattgctt
    15901 caggagtaaa acctcttcgg cgcattcctg ctaaagtagg cattctgggg tcatcccatc
    15961 ctctaactgt cccagattga accagttcca gtagttttct tttactcatt accgtatagg
    16021 aaagatttaa gcgagcaaat tcaatttgtt gtggatgaca gggaactggt aactggtcca
    16081 aaaaccagtc ataaagaggt ctatgatctt caaattccag agtgcaaaga gaatgcgtga
    16141 ttccttctat ggcatctgag atacaatgag tgtaatcata cattggatat atcacccatt
    16201 tatttccagt cctatgatgc tctgcttttc taatgcgata gataacaggg tctcgcatat
    16261 taacattggg agatgccata tctatcttag ctctcaaaca gcgtgaacct tcttcaaatt
    16321 ctcctgcttt cattctgcga aaaaggtcta aattttcctc tacactccga ttacgatagg
    16381 gactttccgt tcccggaacc gttagagtgc cacgagtggc tctaatctct tcttgcgata
    16441 aatcacaaac ataagccttg ccatctttta taagcatctc agcatattca taaagctggt
    16501 caaaataatc ggaagcgtaa aatagtttgt cctgccaatc aaaacctaac cagtgtacat
    16561 cttccattat agattccaca tattccacat cttctttagt gggattagta tcatcaaaac
    16621 gaagatgaca aactcctcca taatctcgtg ccaaaccaaa attaagacaa atagacttgg
    16681 catgacctat atgaagatag ccattgggct cagggggaaa acgagtaact acttttttgc
    16741 attttcctga tgcaagatcg ttttctatta tagtacggat aaaattggaa ggatagtttt
    16801 tttcattttt tttatcgggc atattccata cctttttatc atttatttca taacataatt
    16861 ttgaaaatct atagtaaagt caagctgaat cattaagtag atagatagca attagttatt
    16921 aaaatgatga taatactgga atttctgata gttggagtaa aagttgtaag gtcaatccta
    16981 tcttatagtt aaagaaagag aagaggatag tattacttag attttaattc ttattgaata
    17041 tggttcaagt ataactcaat aataaaacaa ttataccttt ttgtggcggg attacctaat
    17101 aagtatactt ttttctttgg cttacaattg acttatcgca aagtgcttga catatgaaac
    17161 ttatatgatt aaaaggttaa aaggagtttt atatgaaagc taacattcgt aaaacctata
    17221 cttttgatga tgtgttgtta gtacctcaat catctaatat tttgccttct tccgtatcat
    17281 tggaaactaa gataaccaaa actatacaac taaaaatccc tatccttagt gcagctatgg
    17341 atacggtcac agaatatgag atggcaattg ctatggctcg ggaaggaggt atcggtatta
    17401 ttcataagaa tctcagtata gaagagcaag cgaagcaggt gcaattggtt aaaagagcgg
    17461 aaagtggtgt aattaccaat ccttataccc tttcaccgaa tgatagactg acaaaagttt
    17521 gggaactgag agctcaatat aaagtaggtg gatttccagt agtggaaaat ggaaaattag
    17581 taggaattct gaccagtaga gatattcgct ttgaaactga tggtaaaaag ttggtaaagg
    17641 atttaatgac tcccagagaa aaacttatta ctgctacggt aggaacttcc agcgaagagt
    17701 gcattgcttt actacaaaag catcggatag aaaagcttat cctggttaat cctgattttt
    17761 ctttagcagg aatgttgacc gttagggatt tattgaaaaa gattaattat cctaatgccg
    17821 ttcaggatga gaagaataga cttttagtcg gagcagctat aggagttaca ggagactatc
    17881 tggaaagagc tcaggagctg gttcatcagg gtgctaatct attagttttg gatactgctc
    17941 atgctcatca ttccaatatt cgttctgctt tgaacaaact gaaagcaaat ctaaatatag
    18001 agataatggc tggtaatatt gctacggcta aggcagcgga atatctgata gatagtggtg
    18061 cagatgctat taagataggg atcggtcctg gttctatctg tactacaagg gttattgctg
    18121 gtataggtgt acctcaatta agtgctatta tggactgtgc tgaagtggca gaaaaagcag
    18181 ggattcctct tattgctgat ggtggcataa aatattctgg tgatatagtt aaagctctgg
    18241 caggaggagc aaatgccgtt atgattggtt ctttactggc tggaactgat gaaagtcctg
    18301 gtgaagcaat tatctataat ggacgacgct ttaaaagcta ccggggaatg ggctctattg
    18361 gagcaatgaa aaaaggtagc aaagaccgtt attttcagga agaactggaa gaaaataagt
    18421 tggtggcaga aggaattgaa ggaatggttc cctataaagg tccagtcagg gaatttctct
    18481 atcaattaac tggaggtatt aaagcaggaa tgggttattg tggtgcagaa aatttagaag
    18541 ccctgagaaa gaaagcggaa tttattgaaa ttacttctgc tggcttaaga gaatctcatc
    18601 ctcatgatgt caacatcacc aaagaatcgc caaactatca gattagcgaa tgatgataag
    18661 ctcaatgctg aactgataag tcatctaaat agctttgttt attaccttac agtagaaaaa
    18721 gggatggctg aaaacagtgt ggaaagctat cgccgtgatg taagagattt tttagaatat
    18781 aatcctcagc ctctttcaga atacactgct caagatatta caggttatct aactgattta
    18841 atggaagctg gtttggttaa tacttccgta gcaagaaaaa gagtggcttt gaagcaattc
    18901 ttcacttttc tggaagataa tggagagaat atccaggtag actttgctca agtgccgaaa
    18961 attaagatag gtcaacatct tcctgattat cttgatgtgg aaacaatgct taaacttctg
    19021 gatggtttgc caacttctac ccctctggaa aaaagaaata aagtgatgct ggaattgctg
    19081 tatgcgactg gaatgcggat atctgaattg ctaaatttaa ccattcatga tatcaatttt
    19141 tctgaacagg ttattctggt tagaggaaaa ggttcaaagc agcgctttgt tccttttgta
    19201 gatagtgtag cggtgttact tcaagagtac atcaaagatg tgcgtccagt attacttaaa
    19261 tttgaacata cagatatcct ttttctcaat aatcgcagcc aaaaactctc tcgtatgggt
    19321 ttctggaaaa tcttgcgtaa aatcactcaa gaagctggat taaaacagga aattacacct
    19381 catactttcc gtcattcctt tgccactcat ttattagaag cgggagttaa tctaagaatt
    19441 gtccaagctt tacttggaca ttccagtcta aataccactc aaatttacac tcatatagat
    19501 acccgttatt tagtggagac tcacagaatg tatcatccga gagcctgaaa tattataatt
    19561 ttggcaatca attataagca aatagcgatt tcttatctgt ctttaaacct tcgtttaaaa
    19621 cttttcttat gataaaatta gattttcacc gtttcaatct gcataggagt tttcataaat
    19681 aatggtggaa cgaggtcttc ctcgtttcaa actccgcagg agttttctta taggaggaat
    19741 aagtaacttt tttatgaaga aacgaaatat tttcatctca taagatgtgt tcttactttt
    19801 ttctcgtaga aatagaacaa tttatccact ggagtaaaac agccctacga ctttaccaga
    19861 ccggaataat tcccacttcc tttaaatgtt cattaatttt attaattata taatctttgc
    19921 tctttcttgc ctctaacttt gcaaaagaga taggaaagag ctaagggaga gctaagannn
    19981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntaa aacgattatc aattatgtgt
    20101 attcaggaaa agggacgata ctttagtaaa agaaatacag ttcttcctga agagggaaga
    20161 tttcctggta attgataggg cgtattaaaa aggttttgta attgaagaga tagcaataaa
    20221 ttctcttcaa tagggtattg tgcttccaag ctaaggtcaa aaatgaaggg gagatagtta
    20281 tggtcttcat cgtaacggaa atattcttgc tctaaggtgg taattagatt cagattttta
    20341 tattttgatt ttatagaagt attagcagtt aaaagtgggg aatatgattt tctttgccag
    20401 ttcttatcgg gtaagaattc catattcaat ttcagatttt gataacagtt caggtctttt
    20461 acatagaagg aaaattccat ttgaggttga taactaaaca cttcttcaaa acggtaataa
    20521 gtttgaccag atacaggatt agaatagatg gaagggacat tatagctgaa ctttatattt
    20581 tgtccaaagc caattttatt ccagaggcga ttttcgggtt taaaattaat ccagtagaaa
    20641 ttagagagat tcaaaggact catacaaata taatcttttt ctggaacgat agtccaggga
    20701 gagttagatt ttaaatttgc cagagaaaaa gatgtaagtt ctgccttatt ctcaagttcc
    20761 agctgttttc ccggaccggt aatccagcgt ttatgcagat taatagaggg taaaagatga
    20821 ttaaaatcgg tcatcagacc agcttcaaga gcaggatatt tttctggtgt taaatttata
    20881 tgatattgag ctttgattcc ccaggcagaa tcttggaaca ggaataaatt ggagaaagta
    20941 ttatctttaa aggagaaatc gttggaatgg cggattccga aagaaaagtc atccacagaa
    21001 tcctgattat ctttttgatt gaggttttct acattaatcc aggtctgcac ttggttgagt
    21061 gatatcttac ctttagtcag atttttagca ggagcaaaca gggtgtaaga aatcaaagaa
    21121 gaagaaaaac tatcggagga agcttcctga tactgaagat tatgtgcgaa ttcaggtaaa
    21181 ggatgaaaat ctgataaggc gcgaagttgt aagtttttta gagagcgatt agttccaggg
    21241 acagaaaaat tgccatcaaa atctataaaa ggaagttgag aattattagt gaataaagca
    21301 gcatagaggt aagaagaaaa ctcgcttttc attccaatat ttaaagatag aggtttcatt
    21361 tctttatcag taatcgcagg tggcatatat ttagcagtat acactctcgg taaataaggt
    21421 gggatggaat cttgcggtat ctcaaaatta aaattcaaag gttttttaat tagaggtgct
    21481 ttaggtgcca cttctgaact gatttccacg ggaggtaatt tctgagtttg agccacgaga
    21541 acctgtggta taataccaat cagaagaatg agacagggta ataataggaa taaagatttt
    21601 ttcatagtca ttgtttttcg gtaggtaaaa gttgttgcag atgatttatc tgttctttta
    21661 aaaggatatt ttgctcttct tgcagacaga gttgagcttc ttgcagttca ttgagattga
    21721 ttagagtaag gatataaaag taactggcat ctctatagag ttcaggataa tccttataca
    21781 gaagtctaat cttacggaaa ctacgtagag cagaaggata atcttctttt tgataataag
    21841 ttcttccctg ataaaagtca catatagcac ggacataggg ttcagtttgt gtctgtaaca
    21901 aggaatcagc taaagcaaag gtagcatcag ggctaacttt ttcccagaga tattgtagat
    21961 atacaatata agcttgagga ttgtcattta taaaaggagt tccagctttc cagatttcca
    22021 taaaatcttt ataatcatct ttataggaaa gctccagcat ttttagccag gtatcagcat
    22081 caggttttaa ggcgaatgcc tctctatatc gttccagtgc aagctggata tttccattta
    22141 gttcagccag ttcagcccat ttaatcagaa cttcctgact tggtgaggct tcataaattt
    22201 gctgtgccag actttcagct tcggataaac gattcagatt aataagagct tccagcatca
    22261 ggaggtctat atcacttatt tgtttttgag gaagattgag actggttttg aacttattag
    22321 cttctcttag taattcattc catagttcag attctgcgta gagcttaact aatatatatt
    22381 ccagttcaaa tttgatatat tcactatgga aaaggtctat catatctgcg agttcatcaa
    22441 ttttatctgt atctcttagt tttctggcag aaagttctat accggtaaaa acatctgaga
    22501 ggaaaataag ctcttcatcg gttagaaaag tgttactggt aaaattgcgg attactccca
    22561 tccagtaatc aagtgcttct tcatattctc cgagattata atagatatta gccaggtcac
    22621 ttaaagcttg cagataatga tcggactcag ggtatttatc caggaaatta tcatataact
    22681 gtaaggctct attccagttt ttgccttgag ctgcagattt ggcagcttga agtaaaaata
    22741 tttcagcatt cggactgtcc tgtgccagtt caagaaatag atttgtagct tcattgtatc
    22801 ttttaaggta aaaaagagta taggcagaat aggctttagc ttcattttcc tggactttac
    22861 tggtggcaag attttccgct tgtctataaa gcttcaaagc ctgctcataa tctttttgat
    22921 tgaaatgaat ggaggcaagt agaatggatg cttcaaaatt cttagcaggt tccagcacct
    22981 gttccagaat ctgcttggct gagtcatatt tatcctgata ataataggtt tgagctaaac
    23041 gaagatatag gatagaatag ttttctggtg aaggggtgat gtttttgtac aattcttctg
    23101 cggctttata atttgaagct tgataatata tttctgccag cttgaatcgt gcaatatcta
    23161 tccatttaga atggggatat ttctgaatca gttcagtata atagcttttt gctttttctg
    23221 tgtcacctgt cataagagca ctttcagcca gataatatat gatggaatca caaaattttc
    23281 ctgagggata ttttcgtaaa tagttattac aagtttctgc acaggattta taattattaa
    23341 ggtaatacca ggtttctgca caaaacagat aattcagctc tctactcaaa ggttccatag
    23401 gataattgaa ggcggcaagg aagttatgta ttgcgtcctt atagtttcct ttttccagtt
    23461 gccattttcc gagtaattga taggcttctc cacattgtgg tcttttctct ttaatgaatt
    23521 tatcaagttg ctgaaaggcg agatttttat ccttgtgagc aagaattttt aatctttcca
    23581 gataggataa acaggcaaga ttgctatccg atgacttaat cagagtttga aagagcgaat
    23641 ctgatgcaga aatatttcct gttttgagat atacaatagc acgataataa ttagctaagt
    23701 cattgtgaat atttaaacta tccagtaagg cagtagcttg gacgaggtct tcttgattta
    23761 gagccttgcg aacctgtaat aatccgattt gagtggaagc agttgtataa ggactaagtc
    23821 gggttagata ttcatcaaac tctttatagc gtcctgaatt taaaagatgt tccaaccaga
    23881 tgccaaaata gtcaatagtt tcaggtaatt cagcacttaa agaatccagt aaagctgtca
    23941 cggttgaatc tcgttctaaa taaaccaatg tttggaaata gtcagaaaag gcagattttt
    24001 ctcctgaagc taatgctttg tgtagttctt gttctgcaga aagccagagt tcttctctgg
    24061 aatagattct tcccttccat aaatgagctt gagatatata ccagggatga atagcagatt
    24121 tttctaaaga gttcagaagt tggatccctg tcttataatc atcccgataa taacaacaga
    24181 ttgcataatt gagatagata tcattaacga tgtctggagg aaggttctgg ttaattattt
    24241 ttttgtatag taagctactt ttgtgaaaat ctccttgttt tagagctata ttagcgctaa
    24301 gataatccag atatagaaga taaggagaat caggataggt tgcttggaaa actgctatct
    24361 cttttgctgc ttcatccaga tagccatcag aattatagaa catttcaaca atgaaggaga
    24421 aatcttctgt ttctttaaag gattgagaat gtaacggaac caacagaaac aaaatcccta
    24481 taattataca tatcagcttt gttaatgttt ttgaggaaca agggtggtga agaaaagaca
    24541 ttaaattaat atacataatc atttaaaggg tattatcata aagacagaag tgccttcatt
    24601 ttctttactt ttgataataa tttgaccgtt atgaagttct gctattctct tagaaattgc
    24661 caatcccaag cccaatccac tgcttctata ttccacagtt ccgcttttat gtgcatagat
    24721 gtcatttaac tcatagaact tacggtatac attttttaat tgatactctg gaattccgat
    24781 tccattatct tttacataaa tcactaaact atcttgattg ttgatacgtt cctgagggaa
    24841 agccgatttc ctcgcaccta tcacaatagt gccaaattca cgcgtgaaac gtacagcatt
    24901 aaggacgaca ttatatatca ttctgtgcaa ggcttcccag ttgccattta ctggaggtag
    24961 accttcttct atttccactt tgatttgcat cttgcgttga cgagaaagta ttaacacttc
    25021 ctgttgcacg aggtcaatta tctcttctat tttaattggt gattttggaa gtgattgggt
    25081 gatattatat aggttcatag tagttatatc accaatactt gtagcaagtt tatttaccgc
    25141 tgcttccagt ttggaaatga tttcttgacg ctcttcttca tcagaataaa gcttccgttt
    25201 taaacgagaa acataaccct ggatggcagt taaaggtgta ttcaattcgt gtgccacaat
    25261 ttccacaaac tcatccttac tttgttccag attactaagt tcattacttt tttccagtag
    25321 ctcagcatat ttttgagcat ttcttatggc attagccacc tggtcaagga ataacttaat
    25381 aatatcatca tttaaataca tttcacgaga gctatcacca taattatcaa gatagagata
    25441 gccatagttt aggttatcaa caatgatagg acagcagaaa atggtctgaa tagcataatc
    25501 ttgaacgctg atggcatttt taaagcggtt atccagctgt gcattataag ccataatggg
    25561 ttcttgattg gtctgacaaa gacttagagc cgtattacta attccagcag tggatgttaa
    25621 tggttgtttg ttttggtcca tctggacttt atagagatta tttccttctt catctttact
    25681 tatgaaaaaa ccacgagtgc taccagtcag gtcaatagca gcggacacga ttccagcttc
    25741 cagttcgttc atttccagaa tacccaataa atcacgagaa atgttgataa gctgattcat
    25801 tttttccatt cttaaggtaa tttcttcata atcattgatt ctgatgatta gagcagccag
    25861 atgggtttta agactacgag caatagttaa ttctccttga gtgtagggca attctccatt
    25921 atcacttaac accagaaaac caatgcgtcg ggttccagat aaaagaggga ttatgagtat
    25981 attatgctgt tgataacgga aacgaaccag attatctgag gtgaaagatt gttctatata
    26041 gggagaaaat tcagcaggaa gcatcatatc aggatcccag ttatagctta aaaaggcata
    26101 ataagaattc attttttcag agaagaccat cagtttaaat tgccaaggtg aaagcagttg
    26161 agttatgcct ttttctacta agaatttcat ttgctgcaca ctggtcacat tggaaatagt
    26221 aaagataagg tcattaacct gatttcggat atctcttttt cgggaagcaa ccatcactgc
    26281 attgaaatat ttaacattct ttaaaccaaa gaggttcttc cttaaaaagt tttctctgtc
    26341 atcaggactg atatctgagg ttattttatg cagatactgt ttataatcct ggaattcttt
    26401 ttctgcctgt ttaagagcac ctaattctac cagtatctga atcttaatct gccacaattc
    26461 aactattaac tggtaatatt gactttgctg gcattcctct aaaatctgaa tggtcttgcg
    26521 taagatgctc cgaagaggaa tagaagcatc agctaatttg agtttcaatt ccagaatatc
    26581 cagacttttt tcccagtatt tatattgata ttcctgaata gtaggtctgg cattatttat
    26641 tatttcctgt gcctttacat attctcccaa gccatagtaa catttagccc ggagaacatt
    26701 aaaaactgcc atagcataat ggttatttat atcgccagca aattgagaag caagtttaag
    26761 ctcttccaga gctttgtggt aatccttctc tgaaatagcc agaagactga gaacattatg
    26821 atagaattcc tgttcttgaa ttaattcata atcaatatgg gcatttttct gcagtagttt
    26881 ctttaacttt gaaggatagg aaatctcgct tagataatat aaataagttt taaccagggg
    26941 atttatttct tggataacac cttcaatcag tctgggttca tttttgctaa taaattgata
    27001 gtaatgtccg aaacccttga ttttactttt tgccagagca aggttacgaa taaccacatc
    27061 ctcatatttt ttagtcttga gtgaacttaa aatttgagaa gcctccatca attttgcttc
    27121 tgcttcttca aattttccca tcttaatcat agcttcacct tgattaagta gagctctacc
    27181 ttgagcaaga tggtcacctc tttttatggc aaaatccaga gctttttgag aatgattaag
    27241 tcctgaaacg gtgaatcctt gttttaagtg taaatctgcg atattattgt gaatgcgggt
    27301 taaatatcta tctaccctat acttattcca gatattaaga gcctgattaa agtgctcttc
    27361 tgcttctata acattttttt gcagactgta aaattcaccc aattcattat gcaataaagc
    27421 gagattaatc ataacctcta aatcttgatc aggtggcaat tgagcaagaa aatcctctat
    27481 agtgctaatg gcgaggtctc tttggctccg atgaaagaga tataaagcaa ataaattggt
    27541 gtaagtaatt tttaatgcta aatccatctc cagattagct gcgcggtcca gaaacttttt
    27601 cattctaaac gggtcttttt ccatatagca ctgagcctga tgcaaaattg cagtaacctt
    27661 ttgagtagga gttcgagcca atttttctgc cttaataaaa taccgataag cattggtggt
    27721 attatcagaa agtagtttta tggtgcccaa taaaagatat ttttcatatg aatcagggat
    27781 attgttagtt gcttcctcaa taaaatccag tccttgaata tttccggtaa tctctatcat
    27841 cttatgagtt tccgcaaggt cggttaacag ttcttcatca gcaagttcca tatcaggatt
    27901 gaggtcaatt tgtagaacct gaatgatagt tttttgcgct tcttcttgca gataattttc
    27961 ctttagcaat tggaagagat tcaaaagcca ttttcgttca ccaactttat cctgagcaat
    28021 ttgagcatta tggataatac cttcacaggt ggatatatca ttaactttct ttttttcata
    28081 atacttgaga actcttaagg aaaccagctt gtgaattcta aaatcacact cttcaaagaa
    28141 acgttctttt gcttctctga aagtaaagta gtagtattct ccacgttttt ccagaatttc
    28201 attatattta ctttcattca gtaggttata aagctcttca tctttaactt tgcaaatata
    28261 gcgaatcaat tctattgaga tgggcgtttg aaccactgaa agcttctgaa gatgtttata
    28321 gttaacctca gttagatgac tcattcggta atagatagaa tgcaaaagac gacttggaag
    28381 aacataatta tccagatttt ccggataaat taatttaccc tcataacgaa tctgtttctt
    28441 taaagcaaga tctataagta tctctctaat gaaataagga tttccagcag aacgttcgta
    28501 gattatatca caaaaatttt gggaaacttc aggggataat aacttatgaa tatatgctgt
    28561 tgcttcgtcg ggtgtaaaca ttggtacact gatgagcacc gtatgtttaa tttgcttgac
    28621 cttattaaaa tcagttgagc tcataacaat caggatggga tactgagcta cagtagggga
    28681 aaggtaattg ataaaatcta tggtattatg atgcaagtat tgaatgtctc gcacgataaa
    28741 gataatgggt tttctttgtg ccagttcaat taaaacatca cggcaaaatt caaaatcagc
    28801 tctcagttca tcttttgtag gggttatttc tttagctgcc tgctcagaca taaaaacata
    28861 gcgtctcaat tgttcagtca ttgtaggcat tgatttgtag cgttctattt cttccggact
    28921 cagagattgc agatattctt ttattaaagc aaagaaagct tcatggtcag tacgagtgca
    28981 agtgtaatca aagatgaaat aagacccttt cagaatatta taacggaata aggaaagtat
    29041 acttgctttt ccgatgcctt ctcctccaac taaagagata attttaccat ttgaatgctc
    29101 taattgcgga agataatcca gcaactgatt aacaatcttt tcttgcaccg tatagctgtt
    29161 gaatttaata gaattgatga gagaccaaga ttctgagaaa ggatattttt tacctaaggt
    29221 acgattgata taactgatta tttcagcact actctgaaaa cggttttcgg gattgcgttc
    29281 cagcatccgc aaacagagtt tttccagatg cagtggtata tgaggattca gttctgaagg
    29341 aaaaataggt ataaagtatt gtctactacc gcttcggata gcatttatct gttcaagagt
    29401 gaaaggaaaa tttcccgtta ttatcttata aagtatcact ccaagtgagt agaaatcgct
    29461 ggcaggggtg gctggttttc cttgatatat ctctggcgct atataaggta gtgttcccga
    29521 gaccgtttgt gattctttat ccaggtccca gcgagaaaaa ccatagtcta taactttcac
    29581 ttctatctga ttattgcttg ttcggtaaag aacattttcc agtttgaggt ctctgtgcag
    29641 gatattttga gtgtgtaaag catctaaagc atagcagatt tgaactatga tatcgtatag
    29701 cgcattgacc ttactcttgt taaagtggaa actattcaag gtgatgccat caaagaagac
    29761 actgagggaa tagatatggt ctcccacatt gccaaaatct accattttac taagattagg
    29821 atgctctatt ttggttatat ggtgcatatc tttaggctta aaatagcggt aaaattcctc
    29881 ggaagagagg tattgaaata gcttcaaagt atagatatta tcgttacgta tgtctcgcac
    29941 tttatataca ttagcccatc ttcctctgcc taagctttct agaacttcat accggtgatc
    30001 aataaacata tatagtaaac ctcttagtat tctttatttt cagaatggca gggcttgtca
    30061 acttaaaact ttgtgaaaaa ataaaagaat ccctaaaacc agaagatgct tttatagttt
    30121 agtctt
//
LOCUS       DAORZF010000016        28506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_1425_length_28506_cov_353.238023, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000016 DAORZF010000000
VERSION     DAORZF010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 28506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28506
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1425_length_28506_cov_353.238023"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..346)
                     /locus_tag="PK015_03110"
     CDS             complement(197..346)
                     /locus_tag="PK015_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33497.1"
                     /translation="MIRYRNMHGIMGNNRNKSMSSEASGIIKQMIGEMLKTSLTQDEL
                     DELHG"
     gene            complement(459..1967)
                     /gene="malQ"
                     /locus_tag="PK015_03115"
     CDS             complement(459..1967)
                     /gene="malQ"
                     /locus_tag="PK015_03115"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOL33498.1"
                     /translation="MKKNGMLLHISSLPSDFGIGDFGPNAYQFVDYLNDKKFCYWQIL
                     PLYQPGYGNSPYNPVSAFALNPYLISPELLYEDGLIDLPDMEEAKLPSSNQIPYEAVY
                     RLKDNLLKKAANNYLSDIEIYEYIESNAFYLKPYLAYLTLCKLYGDDDWHNFRPEHRH
                     YSDKLYDTLFKNYGSQILRMASIQALAKDQLLRLKDYMNSQGIQLIGDMPLYLSYHSA
                     DVWANQHLFDLDENGNRLNVAGVPPDAFSQDGQLWGNPIYKWDKMKEDGFQLFINRIK
                     NALNYLDKLRLDHFIGYINFWCIPCARDEKTGEPILPENALKGEWKKAAPEDFFPVIM
                     NTFSPEVFIVEDLGILNDEVCYYRDYYKLPGMIILQFCFEEQVPEIRKFPPERYIYTG
                     THDNPTTQEWYANLSDDSPSKINLLRFINEHPLLFSGLGLETEEDIKHSIHLIMCIIA
                     EASGCQNVVLPVQDILGLDASGRMNVPGTALGNWQWRLESFDDLFSTSLPWE"
     gene            complement(1964..2965)
                     /gene="holA"
                     /locus_tag="PK015_03120"
     CDS             complement(1964..2965)
                     /gene="holA"
                     /locus_tag="PK015_03120"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOL33499.1"
                     /translation="MNEINALEIDKLKPKLGENFLLVGGDAYLTDLAIDHIRKHLKSK
                     AKVDLVIIYGDEAKVPEINELLDSYSVFSSAKLVLFRNADQLRKKELDTLADYFREPS
                     EQQSIILTAEKIDNKTAGWKIISKACIHILCNPPNYGGALSPWLDKALTQLGLTMSFK
                     ARQAFISRVELDYANANNELQKLALLVGDKKEITESDVIRSIGSSRVGTQIDFYRALG
                     NRQVKEAMQLLERMLNSEFKALQVLFYLIKFYDTIYYILLLKKAYISAPEIMNIHLPN
                     LYPSQRREFMDFSNNYTLLQLERIFNILLDTDTKIKSSSASDAVLLTNCIFEIMESL"
     gene            complement(2965..3780)
                     /locus_tag="PK015_03125"
     CDS             complement(2965..3780)
                     /locus_tag="PK015_03125"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOL33500.1"
                     /translation="MSFLNAILMGILQGLTEFLPVSSSGHLVLAQHFLGVKESGNILF
                     EVFLHLGTLLAVIIFFRKRIWGLILSLLGKKEYRTRDTAHNDRMIILYLIIATAVTGL
                     VYQILGNPIESLYHKPLVVAFMLIITGIIVFASDYVKKGNISAAKMGLPRSLFIGLTQ
                     GMAIIPGISRSGSTIAASIFSGIKREEAAEFSFLLSIPAILGANLVSFKQFSNLNFNQ
                     LLVYLAGFLAAFVAGYLVIGFLLKLITKAKLKFFAYWCWAIALISIIFLLLER"
     gene            complement(3788..4942)
                     /locus_tag="PK015_03130"
     CDS             complement(3788..4942)
                     /locus_tag="PK015_03130"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HOL33501.1"
                     /translation="MTKQTKYANHHKIKKDIPVGLYLLILLLCGAIIFVIVYKGKGEE
                     IASEFKEPPTKQFSSSELLQTLENKFGLVDGSLQIKTINHKKQLSIPVNPNKMDLTFA
                     NMIVKGEFETRGGKLISGIVEDGGQLLTFNVGTDTYHVKLYYKKPVSIPRKSPRYIAI
                     VVDDFGGIGGDLLQGFLNLPVEIDFAILPGMPYSEETMNKAYEQGRETLVHLPMEPLD
                     YPRQNPGKEAILVQMDKSEVEKIVLKHLNSLPLCSGVNNHMGSLATTDKEIMSYVMEV
                     LRKKGKFFLDSRTSAVSVAYQTAQKAHILAFRNDLFLDSPDISNATMESKIERIKQIS
                     QNRNYVVAITHCHSAEKLKYLQYFISRIQKEGFVLKRLSELKEQEVPAII"
     gene            complement(4917..5780)
                     /locus_tag="PK015_03135"
     CDS             complement(4917..5780)
                     /locus_tag="PK015_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33502.1"
                     /translation="MNKLQKFMIQIFSLLFGDFNGVQVFLYPMTLFCWVDTNSFLYAA
                     TGLLTTQKHFFPLAVFLLIFIFILFLVLKNVSGIYDAIYPLKEGDKAVFRQVVINLHK
                     FLLIVIIAAALLYVVSVFLKYYYDIRIPLKSIYPVAIRFLGIFLLLFYGLRFIWIKPF
                     RENEFDTQKAYIKLRMDYKTHRNLYSAHALFYVLMVIVGAFIYNILILDVLYRIFGWL
                     NFSPNLYFTPPASIFALLYNIFIIAIALLLSNLLFSPLVKAITYIAEKLHPHLYLAKA
                     AADNDETDQIC"
     gene            6012..7193
                     /locus_tag="PK015_03140"
     CDS             6012..7193
                     /locus_tag="PK015_03140"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOL33503.1"
                     /translation="MKNRQCLLLTLMAILLLIAACSSNKSVKVEAPPPPSPLQLAHAY
                     ADSGATAFTAEDYLSAIKYFSKSREYYEEAAPTASPTDSIEVNIERIDLNIALSYVNQ
                     GNEDASLGFYDDALTAYQNAANIYKSLKPLTITPAERDDYLAKLYRNMAITAQNAKKF
                     EQALQYYDMVLTYEPGNEEILNIQYHILKDDIKDQARAYKVLKDYAEASQDYQAYLIL
                     AAAYKDSGDNATAEQYYLKAMELGNNPDVYARVADFYREIKNYTKSNEILTKLIASTT
                     DKSTLALAYRVMADNYAKLGNTAKRIEAYDNSLKYEESAEIALILADHWNKQKNWDKV
                     ITYATKAINANPSNAAAYLLRGNAYYMKKNYAAAKADLQRIQNDPTYGKSASDILKKI
                     K"
     gene            7272..7721
                     /gene="umuD"
                     /locus_tag="PK015_03145"
     CDS             7272..7721
                     /gene="umuD"
                     /locus_tag="PK015_03145"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HOL33504.1"
                     /translation="MKNKIKSDGSITAIYKVSTESKLSRPLLGTKVPAGFPSPASDYL
                     EGTLDLNKYLIAHPASTFFMRVGGDSMIGAGIYPEDIVIVDRSLEATNNKIVVAVYDG
                     ELTLKRLRIENDKYYLVSENDSYPVIEVNKELDFFVWGIVTFVIHQV"
     gene            7725..11000
                     /locus_tag="PK015_03150"
     CDS             7725..11000
                     /locus_tag="PK015_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33505.1"
                     /translation="MRNRKLFALLTLFSLFPASLIFAQGLVVSTNIDLQPECWEYHIS
                     SSPIINGSENVYADSLLLLPGKDYQIDYKRGILKLKNFPEASSLRVDYILVPSSLTQK
                     YYLYEVREPSDSLFTSITPPKPLWQSTDSNLLVSGSKTFALTFSEEEAFDLKQSLFVN
                     LNGELGQNVNITAQLSDSQSKLTPEGDSKELSNLDKVFIRVYGKQYEIAMGDLDWQFD
                     GTRYINYKTTIEGLNAWYGKRHFIQAGYTASSGKPASVKVNIIDGKQGPYYLNPTGFQ
                     STYLIIAGSEKIYCDGKLLERGTDYYIDYSEGSVMFRTMVVSSNRINAYFQYADEFYN
                     QSTYFNSSTFNLLPGFSLSHHFIHQVDAKDHPLLYEFTAADLDSLRKAGDNVVWGNGI
                     VEVESGTGSYIQRINTDGLIYYEYAPYDSLACYNVTFSYVGSGNGDYEEFSSGKFRYI
                     GTGMGAWLPQKRLIPPVKRTNADLLLSYVNDSVELGVEGIFTLNDKNTFSSLNDNDNQ
                     SGLLYAYGNLKKGDMGKENYAGLDFEKRWANSFLFSQDTDISQNYDFALLEQADSLSQ
                     YQINLTLGSRALNWWNPELMLRYRQVQDLFTQRALRFISRSTAKGIIPGLNIHSTVSL
                     QDYDDISIPSSVMQYHDFSSERISRWVQTKLIMNYNSLDYEDNSPLYISTRYYKINPQ
                     LTLGDAKISLTQLGYMRDAIDMKENFWHSVSTSQTYSLKHSTSTLNHTINLEFTHRNV
                     DKKNEMDNPKSKYDLINLRNSNSFFKQAIMILGNYQLNQTEFFPKIRELEYVGSGLGL
                     YDSTGVYTPDGDWDYVYITSDKGTLSTEINGQLSFYFKPGNYFPKWNRIHSDIILQAT
                     EQNPEMKHWQSYLFLPGYVFDKDTTIFGKQSIAPTIWMDIIPNRILGNLNYQYNRTLD
                     KRYQTIARTTESIFGAEMDLKQFGPNNYNLKYELSKEKDTRYMSDITVNALNVLIQHN
                     YSPNTIGTLNLSGFSESGAQQTSSNNYKLQGLGLEPGFRGSWGRKGRVSASVGIRYNQ
                     RSGSEFLTFLPEKREGLLLNWGTSTIYRLNNFCSITLEYSGNSYPQDKVRHTLKMEFK
                     AEL"
     gene            11022..11801
                     /locus_tag="PK015_03155"
     CDS             11022..11801
                     /locus_tag="PK015_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HOL33506.1"
                     /translation="MELISLGVLDYLFVLPFIFLAGLIDSIAGGGGLISLPAYVAAGL
                     PMHTALATNKFSSACGTAFSTANYLHSKVIDLPVALISALLALVGAYLGTRTVLKLSP
                     EPLRYLLIFIIPIITILTFIHKDIGSKNRSTEMKLGIRLFLGAIAGLIIGFWDGFFGP
                     GTGTFLILIYTTIMHYDFMVANGNTKVVNLTSNITALITFLIAGKVYFPLAIPSAICG
                     IAGNILGSKLVILRGNKLIRPVFIIALILLLGRVIYDLIKY"
     gene            11931..12005
                     /locus_tag="PK015_03160"
     tRNA            11931..12005
                     /locus_tag="PK015_03160"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11963..11965,aa:Thr,seq:tgt)
     gene            12014..12099
                     /locus_tag="PK015_03165"
     tRNA            12014..12099
                     /locus_tag="PK015_03165"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12048..12050,aa:Tyr,seq:gta)
     gene            12105..12181
                     /locus_tag="PK015_03170"
     tRNA            12105..12181
                     /locus_tag="PK015_03170"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12139..12141,aa:Gly,seq:tcc)
     gene            12192..12267
                     /locus_tag="PK015_03175"
     tRNA            12192..12267
                     /locus_tag="PK015_03175"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12225..12227,aa:Thr,seq:ggt)
     gene            12319..13503
                     /gene="tuf"
                     /locus_tag="PK015_03180"
     CDS             12319..13503
                     /gene="tuf"
                     /locus_tag="PK015_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOL33507.1"
                     /translation="MAKEKYVRTKPHVNVGTIGHIDHGKTTLTAAITLYLSKKGKAQF
                     RSFDSIDNAPEEKARGITIATAHVEYETDKRHYAHVDCPGHADYIKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVGVPAIVVFMNKCDLVDDPELLDLVEMEVRELLEK
                     YEFPGSEVPVIRGSALKALNGDPEAEKAIQELLDALDNYIPEPVREIDKPFLMPVEDV
                     FSIPGRGTVATGRVDRGTIRVGDKVERVGIKETVETTCTGVEMFRKLLDEAQAGDNIG
                     VLLRGFAKDDVERGMVLAKPKSITPHTKFVGQTYILKEEEGGRKKPFRTGYRPQFYFR
                     TTDVTGTITLPEGVEMVMPGDNVEIIAELITPIALEQGLRFAIREGGRTIGSGVVTKI
                     LE"
     gene            13520..13666
                     /gene="rpmG"
                     /locus_tag="PK015_03185"
     CDS             13520..13666
                     /gene="rpmG"
                     /locus_tag="PK015_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOL33508.1"
                     /translation="MRDIIILACEECKNRNYTTTRNKRKHPDRLAIKKYCPTCRKHTL
                     HKQR"
     gene            13686..13761
                     /locus_tag="PK015_03190"
     tRNA            13686..13761
                     /locus_tag="PK015_03190"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13719..13721,aa:Trp,seq:cca)
     gene            13797..13988
                     /gene="secE"
                     /locus_tag="PK015_03195"
     CDS             13797..13988
                     /gene="secE"
                     /locus_tag="PK015_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOL33509.1"
                     /translation="MKINFGKVGHFFKDVANELKAVSYPTKEDVKEGTIVVIVVSMIV
                     AIFLSLVDFIFGRLMTLIF"
     gene            14008..14544
                     /gene="nusG"
                     /locus_tag="PK015_03200"
     CDS             14008..14544
                     /gene="nusG"
                     /locus_tag="PK015_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOL33510.1"
                     /translation="MQWYVIHTYSSYENKVKQAIEKGLEGTKLRENVGQILVPVRKSY
                     IIRDGKKVEREKKLFTSYVIIEADLTPELRTYILNIAGVTKFLGQSKTHKDPIPLGEE
                     EINRLLGISDREKEPGKAFNFMPGDMVKVTSGPFTDFEGVIQKVADDGTKVVIEVTVF
                     GRKTPVELNSEQIELIKK"
     gene            14560..14991
                     /gene="rplK"
                     /locus_tag="PK015_03205"
     CDS             14560..14991
                     /gene="rplK"
                     /locus_tag="PK015_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOL33511.1"
                     /translation="MAKPKDVMAIVKLQLPAGKATPAPPVGPALGQVGVNIPEFCRQF
                     NEKTADQPGMVFPVVIYVAKNKSFTFEIKTPPASVLIKKEAGLAKGSATPNKVKVGKI
                     SNEQLRSIAQLKMQDMNCNTLESAMSMIAGTARSMGVVIEE"
     gene            15035..15739
                     /gene="rplA"
                     /locus_tag="PK015_03210"
     CDS             15035..15739
                     /gene="rplA"
                     /locus_tag="PK015_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOL33512.1"
                     /translation="MKHSKRYKAAYAQYDRRKRYDLDEAINILKSLPSPKFDETVELH
                     FNLGVDPRKADQQIRNSIVLPHGTGKNPRVLVFAEGEKAEEAKKAGADYVGSDDLIEK
                     IMNGWFDFDIVITTPTMMGRIGKLGRVLGPRGLMPNPKVGTVTMDIAKAVQEAKSGKI
                     TYRVDKFGNLHIIAGKLSFTPEQLKENIKTIIAAVLKDRPTSLKGVYIKSITLCTTMG
                     PGIKLNVASAVQEARS"
     gene            15750..16280
                     /gene="rplJ"
                     /locus_tag="PK015_03215"
     CDS             15750..16280
                     /gene="rplJ"
                     /locus_tag="PK015_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOL33513.1"
                     /translation="MIQHKKQNTVENLKERLAGAKAIILVDYKGINVEQVNKLRNLFR
                     EAQVDYLVQKNTLIKIALNELGINDLDVYLQGPTAVAISKNDEVAPAKVLVKFVKDVM
                     EEADFPKFKGGLISGKVMNQSELQEIAQLPSREELLARLMGSMKAPLSNFVNVSQGII
                     RKFVYAVDALAKQKAS"
     gene            16305..16682
                     /gene="rplL"
                     /locus_tag="PK015_03220"
     CDS             16305..16682
                     /gene="rplL"
                     /locus_tag="PK015_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOL33514.1"
                     /translation="MSDKTNQVIDLISEMTVLELSELVKEMEERFGVSAAAPMTVAAA
                     PAAGTTETQEEKTEFDVVLNSAGEKKINVIKVVREITKLGLKEAKDLVDGAPKVVAEK
                     ISKEEAESIKKKLEEVGASVEIK"
     gene            16869..20657
                     /gene="rpoB"
                     /locus_tag="PK015_03225"
     CDS             16869..20657
                     /gene="rpoB"
                     /locus_tag="PK015_03225"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOL33515.1"
                     /translation="MRKIKSYSRISERFAELGISEVEIPNLLAMQVDSFNDFLQKDVH
                     PKRREKRGLQEVFESVFPIEDNKGNFLLEFEEYNVLQEKYSIDECRERNLTYQAPLKA
                     KLRLSIYEQKEGTREHKDTIEQDVFLGEIPLITEQGTFIINGAERVVISQLQRSPGVY
                     FSEEKHASGKTLYAAKVIPYTGSWLDFDIDIHDVMFVHIDKRRKLPVTTLLRAIGIST
                     NEELRKVFYKTKTLKLKEAKGCYLFNNIVVEGNKEPVAFANEQITDTLIKQLEQFKIK
                     TVEVIDPDYEIARKVLENTIAKDPTNNQEEALKKIYTLMRPGEEAPLEAAKNLVERMF
                     FNEKRYNLAEVGRYKINERLGINVDPKCLTLTVEDFVHIFKTLVNIYNDKDDIDDIDD
                     LSNRRVRTVGELLQDQYKIGLSNVARIIQERMSISNIDEVTIHDLVNSNALVAAVQSF
                     FLTGQLSQFMEQTNPLAALRHKRALSALGPGGLTRERAGFEVRDVHPSHYGRVCPIET
                     PEGPNIGLIVSPTIYSRINRFGFIETPYRKVVDGYVTDEYEYLDASQEAKYIIAQCNV
                     NLDEKRHITDESVFARHKGDFISVDPKNVQFMDVSPQQMVSVSAALIPFLEHDDANRA
                     LMGSNMQRQAVPLINPVAPIVGTGMEKIATMDTSNIAVAPYDGLVTNVTSSYIEIKPL
                     HEEDEALYLGLGNRIELKKFVRTNQDTCNNQRPIVRIGDTVRKGQPISDGACVEDNRL
                     ALGTNMMVAFLPWYGYNYEDAIILSEKVARDDILTSIYIEEMEVLVRNVKNGKEELAY
                     DIPNVPAYALRNLDKTGIIRVGSVIHAGDIIVGKITPKSVEIEPSPEENLMRALFGDR
                     AGDFTNSSLKAKPGMEGVVIDVKVFSRLEEGIEEEEDQEEKKAKLRNDLNLRRQKIEE
                     FKKEKLSAALLGEVAHTIWDEKTNVYFIAPGKKITKSDLARINFKKLDLDAQLVEDPE
                     KNEKIYQDIILKIKQSMEQSENIYRKSLERLKHGDELQYGVRKMVKVYVAKKRKIEVG
                     DKMAGRHGNKGVISIIAPIEDMPFLEDGTPVDIILNPLGVPSRMNIGQIMETHLGMAV
                     KTLGYEIETPIFDGATVQDIEAALQEAGLPTDGKQRLYDGKTGEPYKERVTVGIIYMM
                     KLNHLVADKMHARSTGPYSLITQQPLGGKAQHGGQRLGEMEVWALEAYGAAHNLEEML
                     TIKSDDVDGRNEAFKAITRGENPPPSGVPESFNVLVSELKSLGFDIEFLKEHELNEEK
                     "
     gene            20660..25138
                     /gene="rpoC"
                     /locus_tag="PK015_03230"
     CDS             20660..25138
                     /gene="rpoC"
                     /locus_tag="PK015_03230"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOL33516.1"
                     /translation="MLKDNPHEIKPNDFDFVRIKIASPETIINEWSHGEVTKPDTLNY
                     RTLKPEKDGLFCERIFGPERDYECACGKYKKKRFQNVVCDRCGVLVTTSRVRRTRMGH
                     ISLAVPIAHIWFVKSTPSKIGTLLDMTVKDLERVIYYESYIVVDPGDSPYEKYELIDI
                     DEYSEIKDKVGDNFLAMMGAEAIRELLSRLDLNNEALNIRSRLKMEKSTLYKQKLINK
                     LKIVDAFLKSGNKPEDMILLALPVLPPTLRPLVPLEGGRFATADFNELYRRVITRNNR
                     LKYLMEIRSPEVILRNEKRMLQEAVDALIDNSRKARPIKGRGNRPLKSLSDQLKGKQG
                     RFRQNLLGKRVDYSGRSVITVGPELKLYECGLPKDMAVELFKPYLIERLQKLGEADKV
                     KNAKKLIEKKQPEIWSILEEVIKDYPVLLNRAPTLHRLGIEAFMPVLTDNKAIQLHPM
                     VCVPFNADFDGDQMGVYIPLSYEAQIEARTLMLSTRNLLLPSNGRLAMAANQDIVLGC
                     YYLTMEEFPEPEDITTLRHFYSTDEVIAAFEAEEQLFSVKGDVEEERSLNLHTWIRVK
                     IKNNYIVTTVGRVIFNQIIPEEVGFQNSVFDKGKINDLAMLCYDAVGQWKTAQFLDNL
                     KELGFHYSTHAGVTFSLSDIVVPKRKDEIIAEAEEEVKKVMDLHMKGGITENERFGRI
                     VDLWKKTTVRVTDEMMEELAKNRNGLNSIYMMYKSGARGSKDQIKQVGGMRGLMDKPA
                     KIGSTGGVDVIETPIKSNFRDGLTVLEYFISTHGARKGLADTALKTADAGYLTRRLVD
                     VAQNAIITIDDCGTTKGVDMSVLKEGNEVVATLADRIQGRVALEDVIDPVTGNILVHS
                     GEEISNKMARTIQNHGVIKVKVRSVLTCEAEHGICAKCYGRNLATQKPAVIGDPVGII
                     AAQSIGEPGTQLTLRTFHIGGAASTATELAEVVSAHDGIVKFDRMNTVTNVNNQLISV
                     SHLGKILICDENDPSKILEEYRVEYGATIHVRDGQKIAMNTKLMSWDQFNNPLISTAK
                     GQLHYENFIKDITYKEEFNDITFSRDITIIESKDRKKQPQFRIVSEDGTSVQVPLPTG
                     LIVKVEDGSFVHPGDILGQTSRMTIKQRDITGGLPRVQDLFEARVPKDKACISDIDGI
                     VTIGGLKKNGREIFVTPPNGLMAPDDGKVIVQSDEYVNRIILLTDKTVYANNDGKVSI
                     IEENKKKKVAIAKRNQKPQVYEIPRGLHIIVNEGDNVKVGAPLCGKILEIPPLQESIV
                     STGDTVKKGQPLTGRKYIIPSGKRIIVHQGDYVESGDALSDGPLDPHDMLVKGVIEAQ
                     MMILNEVQEIYRKQGVKIDDKHISVIIRQMFKKVRITDSGSTSFLEGDIVDKTLVEKE
                     NKEVQKYGKTPAQFEQLLLGITKVSLLTDSWLSAASFQETTNVLTKAAIEGSVDKLEG
                     LKESVIIGHRIPVGTGTKIYNKMIEEAVNSGATVAEIIERFAHPKKEEESEDIYNF"
     gene            25160..25537
                     /gene="rpsL"
                     /locus_tag="PK015_03235"
     CDS             25160..25537
                     /gene="rpsL"
                     /locus_tag="PK015_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOL33517.1"
                     /translation="MPTINQLVRNGRTKIEKKKKNRALMGCPQRRGVCTRVYTTTPKK
                     PNSALRKVARVRLTNGFEVTAYIGGEGHNLQEHSIVLVRGGRVKDLPGVRYHIVRGAL
                     DAAGVEKRVNSRSKYGTKRPKKA"
     gene            25556..26026
                     /gene="rpsG"
                     /locus_tag="PK015_03240"
     CDS             25556..26026
                     /gene="rpsG"
                     /locus_tag="PK015_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOL33518.1"
                     /translation="MPRKKKAVVREVLPDPIYQDVVLSKFINCVMKKGKKSIAEKIVY
                     GAFNIIEEKTKQPPLEVFKTAINNVRPQLKVVSRRVGGSNYQIPMEVSEKNGQALAFR
                     WIITYARARNEKSMDEKLAAELIAASKNEGASIKKREDTHKMAEANKAFAHLRF"
     gene            26202..28484
                     /locus_tag="PK015_03245"
     CDS             26202..28484
                     /locus_tag="PK015_03245"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HOL33519.1"
                     /translation="MNTSMFSRLHIILLALLIFSLSLPVFAEFVQPQEAKIIAQTWLN
                     LIDGKNYSELNIQQVLEYRDNNFTLSQSKYELTDENLPPLYLILFHNNGFVLISAEDN
                     SIPVLAYSSDSSCNINSYPPAFLEWVENYAVQIRQIRDEKTVIPENAQLWQSLFRGNL
                     PANFRQDRAVRPLIVTNWDQGWPYNELCPADPNGPGGHVYAGCVATAMGMVMKYWSHP
                     TTGVGSHSYYCPGYGYQSANFGATTYLWDEMPNSISTSCIPIATLLYHCGVAVNMGYS
                     VDGSGAQSTDAANALVNYFRYPNAVLQNKMGMSDTQWNNLLQTQLDNGCPMYYSGSGS
                     GGGHAFVIDGYQTPGYYHFNFGWSGSSNGYYYINNINPGGYDFNSWNSVIANSIPQNY
                     NINQVRINMNAFGATVGTNFNLTVSTFPVLGSWNVNHFEFTLIYDSDNITFNGASIAN
                     GIASDGNLSVSETEPGYLQVSWNGNSNIIGSGDLITFSFRPLDTGQYLFDMVGMTYNN
                     TPITTTSFIMVDVVPPVTNLTESAITMLNVMHLAYNQIGTTEIRTTYLLPSWNVTHYQ
                     FDLNYDASKLQFMGIDTAGTISAGCEPSVVINNPGSISISCDSNNRFTGDGALLKFHF
                     KAIGNGPTVSITQVSPANFFYNDTQIFNLGSANFILSAYTANDDELAPEITSLQIFPN
                     PFINSTQIKLNSKSSEPVQFTIYNLKGQKVSSLVITDSPKTFTWIPKDMMGKPLPTGV
                     YLLSWEQGKEKGTAKLLYFK"
BASE COUNT         9315 a         5102 c         5568 g         8521 t
ORIGIN      
        1 ggctcatttt tgtatcgtat ccaatctgct cgtctttgat ccatggtaca aaggccttga
       61 tgtatttccg tgcatcgtcc aagctattgg acttgatgtc cagagccata agaacgttta
      121 gggaatatat cttattttag gaagccagtg cccggcattg gtttttcata atatactttc
      181 ttgatcgatt tacattttag ccatgtagtt catctaactc gtcttgggtc aaactagtct
      241 ttaacatctc ccctatcatt tgtttgatga tacccgaagc ctctgagctc atagatttgt
      301 tgcgattatt acccataata ccatgcatat tcctataacg tatcaaacat agtttaacaa
      361 gctttatcat tttcttcttt ataagctttc tttgggttat atttatgatc tccgggtagg
      421 gagattcccc ttccacttac taaccaaaat aacgaatttc actcccaggg taaagatgtg
      481 ctgaataaat catcaaaact ttccaatctc cattgccagt tacctaaggc agtaccagga
      541 acattcattc tgccagaagc gtccagaccc agaatatcct gtaccggtaa aactacattc
      601 tgacaacctg aagcttcagc tatgatgcac attataaggt ggatagaatg cttgatgtcc
      661 tcctctgttt ccagacctaa tccagagaag agaagcggat gctcattaat aaatcgcagc
      721 aaattaatct tactgggtga atcatcactt agatttgcat accattcctg tgtggtcgga
      781 ttgtcatgag tcccggtata gatatatcgt tctggaggaa attttctaat ctcgggaacc
      841 tgttcttcaa agcagaactg caggataatc ataccgggca atttataata atcacgataa
      901 taacaaacct catcattcag aatacccagg tcctcaacaa tgaacacttc tggagaaaaa
      961 gtattcatta tcacgggaaa aaagtcctct ggagctgcct ttttccactc accctttaag
     1021 gcattctcag gtaaaatagg ttctcctgtc ttctcatcac gagcacaggg aatacaccaa
     1081 aaattaatat aaccaataaa atggtctaaa cgaagtttat ccagatagtt cagggcattt
     1141 tttattctat tgataaaaag ctgaaatcca tcttccttca ttttatccca tttataaata
     1201 ggattgcccc agagttgtcc atcctgacta aaggcatcgg gtggaactcc tgccacattc
     1261 agacgattcc cattttcatc caggtcaaat aaatgttgat tagcccagac atcagcgcta
     1321 tgataggaaa gataaagtgg catatcccct atcagctgta taccctgaga attcatataa
     1381 tccttcaatc gcaaaagctg gtcctttgcc agagcttgga tacttgccat tctcaggatt
     1441 tgacttccgt aattcttaaa tagagtgtca taaagtttat cagagtaatg tctgtgttct
     1501 ggacggaagt tatgccaatc atcatccccg taaagcttac ataatgttaa ataagcaaga
     1561 tatggtttta aataaaaagc attactttct atgtattcat atatttcaat gtctgataga
     1621 tagttattag ctgctttttt gagtaaatta tccttcaaac gataaactgc ttcatagggt
     1681 atttgattag atgagggtag ctttgcctct tccatatcag gtaaatcaat caatccatcc
     1741 tcataaagaa gttccggact aatcagataa ggatttaaag caaaggcaga aactggatta
     1801 taaggtgaat tcccataacc tggttgataa agtggtaaaa tctgccagta gcagaatttt
     1861 ttatcattca aataatcaac gaattgataa gcatttggtc caaaatctcc aataccaaaa
     1921 tcagatggta aggagctaat atgtaaaagc ataccatttt tcttcatagg gattccataa
     1981 tctcaaatat acaattggta agcaagacag cgtcacttgc agaactggat ttaatttttg
     2041 tatctgtatc cagtaaaata ttgaaaattc gttctaattg aagtaaagtg taattattag
     2101 agaaatccat aaattctctt ctttgagaag ggtaaagatt aggaaggtgt atattcataa
     2161 tttcaggggc gctgatatat gcttttttta gtaggaggat ataatatata gtatcataaa
     2221 atttaataag gtagaatagc acctgcagag ctttaaattc agaatttagc atcctttcca
     2281 gcagttgcat agcctctttc acttgcctgt tacccagtgc acgataaaag tcaatctgag
     2341 ttccgactcg tgaagaacca atactacgaa tcacatcgct ttccgtaatt tcttttttat
     2401 ctccaaccag aagagccagt ttttgcaact cattattagc attagcataa tccaattcta
     2461 cccgactaat gaaagcttgt ctggctttaa aggacatagt taatcctaac tgggtaagtg
     2521 ctttatccag ccagggtgaa agagctccac cgtagtttgg cggattacaa agaatgtgaa
     2581 tgcaagcctt actaatgatt ttccatcccg cggttttatt atcaattttt tctgctgtca
     2641 ggattatact ttgttgttct gaaggttcac gaaaataatc agctaaagta tccaattcct
     2701 tttttctcaa ttggtctgca ttccggaaga ggacaagttt tgcactggaa aaaaccgaat
     2761 aagaatccag cagttcattg atttcgggga ctttagcttc atctccataa ataataacca
     2821 ggtctacttt tgctttggat ttcaagtgct ttctaatatg gtcaatagct aaatcagtta
     2881 gataagcatc tccacccaca agcagaaagt tttcacctaa tttaggtttc aatttatcaa
     2941 tttccagagc atttatttca ttcattatct ttccagcaaa agaaaaatga tactgattaa
     3001 agctatagcc cagcaccagt aagcaaaaaa tttaagttta gccttagtaa ttagtttgag
     3061 taagaaacca attaccagat atccagccac aaaagctgct aaaaatccag ctaaataaac
     3121 aagtaattga ttgaaattga ggttactaaa ctgtttaaaa ctaaccagat ttgcccccag
     3181 gattgctggt atgcttaaaa ggaatgagaa ttctgctgct tcttcacgtt taataccact
     3241 aaaaatagaa gcagcaatag ttgaaccgga gcgtgaaatt ccaggaataa tagccatacc
     3301 ctgagttaat cctataaata ggcttcttgg tagccccatc ttcgcagctg aaatatttcc
     3361 ttttttaaca taatcagaag caaaaactat aatacccgtg ataattaaca taaaagccac
     3421 aactaatggt ttatgataaa gagattctat aggattcccc aagatctgat acactaaacc
     3481 agtaactgct gtcgcaataa tcaaatatag aataatcata cggtcattat gagcggtatc
     3541 cctggttctg tattcctttt tccctaagag agagagaatc aatccccaga tacgcttgcg
     3601 aaaaaagata atcaccgcaa gtaaggttcc aagatggaga aatacttcaa ataatatatt
     3661 tccactttct tttacaccta aaaaatgctg tgccagaacc agatgacccg aactactaac
     3721 tggtaaaaat tccgttaatc cttgtaaaat tcccatcaat atagcattta aaaaactcat
     3781 actttcctca tattatagca ggcacttctt gttctttcag ttctgagagc cttttaagga
     3841 caaatccttc tttctggatg cgggatataa aatactgtaa atattttaac ttttctgctg
     3901 aatgacaatg cgtgatagct acaacataat ttctgttctg ggaaatttgt tttattctct
     3961 caattttact ttccatagta gcattgctta tatctggaga atccagaaag aggtcgtttc
     4021 ggaaagctaa aatatgagct ttttgagcag tttgataagc aacggaaact gcagaagtgc
     4081 gactatccag aaagaatttt ccttttttac gcagaacttc cataacatag ctcataatct
     4141 ctttatcggt tgtagcaaga ctgcccatat gattgtttac tccactacat aagggcaaag
     4201 aattcaaatg tttgaggact atcttttcca cttctgattt atccatttga actaaaatag
     4261 cctccttccc tggattctga cgaggataat ccaaaggttc cataggtaaa tgcactaagg
     4321 tttctcgtcc ctgttcatag gctttattca tcgtttcttc actatatggc atcccaggaa
     4381 gaatagcaaa atctatttct actggtaaat tcaaaaaacc ttgcaataaa tctcctccga
     4441 tgccaccaaa atcgtcaact acaatggcaa tatatcttgg agattttctt ggtattgaaa
     4501 caggtttctt gtaatataat ttcacatgat aggtatctgt tccaacatta aaagttagaa
     4561 gctgtccacc atcttcaact ataccggaaa tgagctttcc tccacgagtc tcaaactcac
     4621 ctttgactat catattggca aaagtgaggt ccattttatt cggatttacc ggaatgctta
     4681 attgtttttt atgatttata gtttttatct gcaaagaacc atccaccagt ccaaacttat
     4741 tctccagtgt ttgtaagagc tcggaagagc taaattgttt agttggaggt tctttaaatt
     4801 cagaagctat ttcctctccc tttcctttat aaactataac aaagataata gctccacaaa
     4861 gtaaaaggat aagtagatat agacctaccg gaatgtcctt ttttatttta tggtgattag
     4921 catatttggt ctgtttcgtc attatctgct gcagctttag ctaaatataa atgagggtgt
     4981 aacttttcgg caatataggt gatagcttta accagaggtg aaaataaaag attggaaaga
     5041 agcagagcta tggcaattat aaaaatgttg taaagtagag caaaaatgct tgcagggggc
     5101 gtaaaataaa gattaggtga aaagttcagc cagccaaaaa tcctatataa aacatccagt
     5161 attaggatat tatagataaa cgctccgact attaccatca atacatagaa cagggcatgc
     5221 gcagaataaa gatttctatg tgttttataa tccatacgca gtttaatata agctttttgt
     5281 gtatcaaatt cattttctct gaatggtttt atccagatga atctcaaacc atagaacaga
     5341 agcagaaaaa tgcctaaaaa tcttatagcc acaggataga tactttttaa aggtattctt
     5401 atatcgtaat aatattttaa aaatacagat accacataaa gaagagcagc tgcaattata
     5461 actatcagca ggaatttatg aagatttatt accacctgcc taaatactgc tttatctcct
     5521 tcctttaaag gatagatagc atcatatata ccactaacat ttttcaaaac taaaaatagt
     5581 ataaaaatga atatcaataa aaaaaccgcc agtggaaaga aatgtttttg tgtggttaat
     5641 aaacctgtag cagcataaag aaaactattg gtatctaccc aacaaaagag agtcatagga
     5701 tacaggaata cctgaactcc attaaaatct ccaaaaagta gactaaatat ctgtatcata
     5761 aacttttgta atttgttcat attcttttta atcgttgatt ttttatcctt ctttttacca
     5821 atgttcaaag aacttctttt tgtcaatgcc aatgtttcgc caaaattaat gcctctttct
     5881 atttttctca agaaaaatgc taaaaagggg cattatttct atcaatttta tgggttcaaa
     5941 tggattttat attgacaaat cagcaaaaat atggagattg taatcaagaa aaaaaagaaa
     6001 gggaggtaca aatgaaaaac agacaatgtc tcttactgac ccttatggca atattattgc
     6061 ttatagcggc ttgcagttcc aataagagtg taaaagttga agctccaccc ccaccttcac
     6121 ctttacaatt agctcatgct tatgctgata gtggagcaac tgcttttacc gctgaagatt
     6181 atctatctgc aataaagtat tttagtaaat caagagagta ttatgaagaa gcagcaccga
     6241 cagcttcacc tactgattct attgaagtaa atattgaacg gatcgacctt aatattgctc
     6301 tcagctatgt aaatcaagga aatgaagatg caagtctggg gttctatgat gatgctttga
     6361 ccgcttatca gaatgccgcc aatatttaca agagcctgaa accacttacc attacacctg
     6421 cagaaagaga tgattatctc gccaagcttt atcgtaatat ggcaatcact gctcaaaatg
     6481 caaaaaaatt tgaacaagcc ttgcaatatt atgatatggt tctcacctat gagccaggaa
     6541 atgaagaaat ccttaatatc caatatcaca tcctgaaaga tgatattaaa gaccaggcta
     6601 gagcttataa ggtattgaaa gattatgctg aagcttctca ggactaccaa gcctatttga
     6661 ttctggctgc ggcttataaa gatagtggtg ataatgctac agcggaacaa tattatctca
     6721 aggcaatgga attgggaaat aatcctgatg tttacgcgag ggtagcagat ttttatcgtg
     6781 agattaaaaa ttatactaaa tccaatgaga ttcttacaaa acttattgcc agcacaacag
     6841 ataagtctac cttagctctt gcttaccggg taatggcgga taactatgct aaattaggaa
     6901 atacggcaaa gaggattgaa gcatatgaca attcccttaa atatgaagaa agtgctgaaa
     6961 tcgcattgat tttagcagac cactggaaca aacaaaagaa ctgggataaa gttataactt
     7021 atgccactaa agcaatcaat gccaatccat caaatgcagc agcttatctt ctaagaggta
     7081 atgcttacta tatgaaaaag aattatgctg ctgctaaagc agacctgcaa cggatccaga
     7141 atgatccaac ctatggcaag agtgcctctg atatccttaa aaaaattaaa tagtaatagg
     7201 gtatttaaca gtcaaccgtc ttatcttgaa caccttaaag atgaatacgg ttgactgttt
     7261 ttcttttatc tatgaagaat aaaattaaaa gtgatggttc tataaccgct atttataaag
     7321 taagtaccga gagtaaatta agtagaccct tacttggtac aaaagtccct gctggttttc
     7381 cttcaccagc aagtgattat ctggaaggga ccttagatct aaataaatat cttatagctc
     7441 atcctgcttc tacttttttt atgagagtag gaggcgattc tatgatagga gctggcattt
     7501 atccagaaga tattgtcata gttgaccgtt ccttagaagc tactaacaat aaaattgtcg
     7561 ttgcagtata tgatggagaa ctaactctaa aaagattacg cattgaaaat gacaaatatt
     7621 atcttgtttc agagaacgat tcttatccag ttatagaggt taacaaagag ttagatttct
     7681 ttgtttgggg tatagttact tttgtaattc atcaagtata aagtgtgcgt aatcgcaaat
     7741 tatttgccct gctgactcta ttttctctgt tccctgcctc tctaatcttt gcacaaggtc
     7801 tggttgttag caccaatata gatttacaac ctgaatgctg ggaatatcat atttcttctt
     7861 cacccataat aaatggctca gaaaatgtct atgcagattc tcttctcctt cttccaggaa
     7921 aagactatca gatagattat aaacgaggta ttctaaaatt aaagaatttc cctgaagcct
     7981 cgtctttaag agtagattat attttagtgc cctcttctct aactcaaaaa tactatctat
     8041 atgaagttag agagccctcg gacagcttat ttacttctat aactccacca aaacctctct
     8101 ggcaatcaac ggatagtaat cttctggtct ctggctctaa gacttttgcc ctaactttct
     8161 ctgaggaaga ggcttttgac ctgaaacaat cgttgtttgt taatttaaat ggagaattgg
     8221 ggcaaaatgt taatattact gcccaacttt ctgatagtca atcaaaactt actccagaag
     8281 gtgactctaa ggaacttagc aatcttgata aagtttttat ccgcgtctat gggaaacaat
     8341 atgaaattgc tatgggagac ctggattggc agtttgatgg gactcgttac attaattaca
     8401 agactactat tgagggtttg aatgcttggt atgggaaaag acattttatt caggctggtt
     8461 atactgcttc ttctgggaaa cctgcttcag taaaggttaa tataatagat ggaaaacaag
     8521 gaccctatta tcttaatcct actggatttc agagcactta tctaattatt gcaggaagtg
     8581 aaaaaatcta ttgcgatgga aaattattag aacgaggaac agattattat atagattact
     8641 ctgaagggtc agtaatgttt cggactatgg tagtatcaag taatcggatt aatgcatatt
     8701 ttcaatacgc agatgagttt tacaatcaat ctacttactt caattcttcc acctttaatc
     8761 ttttgcctgg tttctcttta tcccatcatt ttatccatca agtagatgct aaagaccatc
     8821 ctttactata tgaatttact gctgcagacc ttgattcttt gagaaaagct ggagataatg
     8881 tggtttgggg taatggaatc gtggaagttg aaagtggaac cggaagctat attcaaagaa
     8941 taaataccga tggattaatc tattatgaat acgcacccta tgatagctta gcctgctata
     9001 atgtaacctt tagttatgtg ggttctggta atggggatta tgaagaattt tccagtggta
     9061 agtttagata tattggaacc ggaatgggtg cgtggcttcc gcaaaaaaga ttgatacctc
     9121 cggtaaaaag aactaatgca gacctattac ttagctatgt taacgattcc gtagaattag
     9181 gagtggaagg catcttcacc cttaatgata aaaatacctt ttcttcttta aatgataatg
     9241 acaatcaaag cggtctactg tatgcttatg gaaatcttaa aaaaggagat atgggcaagg
     9301 aaaattatgc cggattagat tttgagaagc gttgggctaa tagttttctc ttttcacagg
     9361 ataccgatat atctcagaat tacgattttg ctttgctgga acaagctgat tcccttagtc
     9421 aataccaaat caatttaacc ttaggaagta gggctttgaa ctggtggaat cccgaattaa
     9481 tgctgcgtta tagacaagtg caagacctat ttactcagag agcattaaga tttatttccc
     9541 gctccaccgc aaaaggcata ataccaggat tgaacataca ttcaactgtc tctttacagg
     9601 attatgatga tatttctatt ccctcaagtg ttatgcaata ccatgatttt agctccgaaa
     9661 ggatttcccg ctgggtacaa actaaactta taatgaacta taatagcctg gattatgaag
     9721 ataactctcc cttatatatc agcactcgct attataaaat taaccctcaa ttaactttgg
     9781 gagatgcaaa aataagcttg acgcagcttg gttatatgag ggatgctatc gatatgaaag
     9841 aaaatttctg gcattctgtt tctacctcac aaacttatag cttaaagcat agcacttcca
     9901 ctcttaatca tacaattaat ctggaattta ctcatcgtaa tgtagataaa aaaaatgaaa
     9961 tggataatcc caaaagcaaa tatgacctca taaatttacg caattccaat tcctttttta
    10021 aacaggctat tatgattttg ggaaattatc agctcaatca aacagaattc tttccaaaaa
    10081 ttagagaatt ggaatatgtg ggtagtggtt taggcctata tgatagcaca ggagtctaca
    10141 ctcctgatgg agattgggat tatgtgtata tcaccagtga taaaggaacc ttgagtacag
    10201 aaattaatgg acaactgagc ttttatttca aaccgggaaa ttatttccct aaatggaatc
    10261 ggatacatag tgatattatt ctgcaagcta ccgaacaaaa tccagagatg aaacattggc
    10321 aaagctatct atttcttccc ggctatgtct ttgacaaaga taccactatc ttcggaaaac
    10381 aaagtattgc tcccactatc tggatggata ttataccaaa tcggatttta ggaaacttga
    10441 actatcagta taaccgaact ctggataaac gctatcagac tatagctcgt actaccgaat
    10501 ctatcttcgg agcagaaatg gatttaaaac aatttggacc caataattat aatcttaaat
    10561 atgaacttag taaagaaaaa gatactcgct atatgtctga tatcacagtt aatgccctca
    10621 atgttttaat tcagcacaac tattcaccaa atacaatagg aaccttaaat ctttccggct
    10681 tttctgaaag tggtgcacaa caaacaagct caaacaatta taaacttcaa ggtctgggtc
    10741 ttgaacctgg ttttagagga agttggggca gaaagggccg tgtatctgcc tcagttggta
    10801 ttcgctataa tcagcgttct ggaagtgaat ttctaacttt tttacctgaa aaaagagaag
    10861 gattattatt aaactggggc acttccacaa tctatcgttt aaataatttc tgttctataa
    10921 ctctggaata tagtggtaat tcctatcctc aagataaagt gagacatact ctgaagatgg
    10981 aatttaaggc tgagttataa tactatggag aataaatctt aatggaattg atttctttag
    11041 gagttctgga ttatcttttc gttcttcctt ttattttctt agctggattg attgattcta
    11101 ttgctggagg aggaggattg atttctcttc cagcttatgt tgcagctgga ttaccaatgc
    11161 acacagctct ggctacaaat aaattctctt cagcttgtgg aacagctttt agtacagcaa
    11221 actatctaca ttcaaaagtt atagaccttc cagtggcttt gataagtgct ctattagctc
    11281 tggttggtgc ttatctggga acaagaacag tgcttaaact ttctcccgaa cctcttcgct
    11341 acctgcttat ttttattatt ccaatcatca ctattttaac ctttatccat aaagatattg
    11401 gcagtaaaaa taggtctact gagatgaaat tgggcattcg cttattctta ggagctattg
    11461 ccggtttaat aattggattc tgggatggtt tttttggtcc tggtacagga acttttctca
    11521 ttttaattta tactacgatt atgcactatg attttatggt cgctaatgga aataccaagg
    11581 tagtcaattt gacttccaat attactgcat tgataacttt cttgattgct ggtaaggtat
    11641 atttcccact ggcaattccc tcagccattt gtggtatagc aggtaatatc ctcggttcaa
    11701 aattagttat ccttagaggc aataaactaa ttagaccagt tttcattata gctctaatat
    11761 tattattagg tagagtgatt tacgacttaa ttaagtatta aatagagtat atctaaaatt
    11821 aaggagaatc taccaagacc ttttggtaga aaatgaatag catttttcca taaatcgtat
    11881 taaagatatt gacaaaaaaa aggcaataaa attaagtgat aaaatatagt gccgatgtag
    11941 ctcaatggaa gagcaattga tttgtaatca ataggttggg ggttcaactc ccttcatcgg
    12001 ctccagatat tttggacagg tggtcgagtg gttaatggca ctgggctgta aacccagcga
    12061 cgaaagtcta cggaggttcg aatccttccc tgtccaccat ttaagcggga atagctcagt
    12121 tggctagagc atcagccttc caagctgagg gtcgcgggtt cgaaccccgt ttcccgctcc
    12181 agtaaaaaag agctcaagta gctcagtcgg tagagcacct ccttggtaag gaggaggtca
    12241 tcggttcaag tccgatcttg agctccattt aaaaatgata acgattatag aatatagaaa
    12301 cagattccag gaggaaaaat ggcaaaagag aaatatgtgc gcactaagcc gcatgtcaat
    12361 gtcggtacaa taggtcatat tgaccatgga aagacaacgc tcaccgcagc gataacttta
    12421 tatttgagca agaaaggcaa ggctcaattt cgttcttttg atagcataga taatgcaccc
    12481 gaggaaaaag ctcgtggtat aactattgct actgcacatg tggaatatga aactgataag
    12541 cgacactatg cccatgtgga ttgccccggt catgctgatt atatcaaaaa tatgataact
    12601 ggcgctgctc aaatggacgg tgctatcctt gtggttagcg cagcagatgg tcctatgccg
    12661 caaactcgtg aacatatctt gcttgctcgt caggttggtg ttccagcaat tgtagtattt
    12721 atgaataaat gtgacttagt tgacgacccc gagttgcttg atttagtgga aatggaagtt
    12781 cgtgaattat tagaaaaata tgaatttccg ggaagtgaag tacccgttat tcgtggttcc
    12841 gctctgaagg ctttaaatgg tgatccggaa gcggaaaagg ctatccaaga attacttgat
    12901 gctctggata actacatccc agagcccgta cgtgaaatag ataagccttt cttaatgccc
    12961 gttgaagatg tattttcaat tccaggtcgt ggaactgtgg ctaccggtcg tgtagatcgt
    13021 ggcactatca gagttggaga taaagttgag cgtgtaggta tcaaagaaac ggttgaaacc
    13081 acctgcacag gcgtggaaat gttccgtaaa ttattggatg aagcgcaagc aggagataac
    13141 attggtgtgc tcttacgcgg ttttgccaaa gatgatgtag aacgcggtat ggttcttgcc
    13201 aagcccaaat ctatcactcc acacactaag ttcgttggtc aaacttatat tctaaaagaa
    13261 gaagaaggtg gacgtaagaa accctttaga acaggatatc gtcctcaatt ttacttccgc
    13321 accaccgatg tgaccggaac tatcactctt cccgaaggtg ttgaaatggt tatgcccggt
    13381 gataatgtag aaataattgc tgaactgata actcctattg ctttagagca aggactacgc
    13441 tttgctattc gcgaaggtgg tcggactatt ggtagcggtg ttgtcacaaa aatattagaa
    13501 tagagataga agcggagaga tgagagatat aattattctt gcttgcgaag aatgtaagaa
    13561 ccgtaactac actactacac ggaataaacg taagcatccg gatagattag caataaagaa
    13621 atactgtccc acttgccgga aacataccct acacaaacaa cgctaataat tgtataatat
    13681 gttgcaggac agtagttcaa ctggtagagc accggtctcc aaaaccgggg gttgcgggtt
    13741 cgattcctgc ctgtcctgcc acaatctaaa attgaaaaat aaatactggt gaaaaaatga
    13801 aaataaattt tggcaaagtt ggccattttt tcaaagatgt agcgaacgaa ttaaaagcag
    13861 tgtcctaccc gactaaagaa gatgtaaagg aaggcactat agttgtaatt gtagtttcta
    13921 tgattgtagc tatcttttta agtttagtag actttatttt cggtcgcctg atgactctta
    13981 tcttttaggg ggaatataat cgtggcaatg cagtggtatg taattcatac ctattcttct
    14041 tacgagaata aggttaaaca agcgattgaa aaagggttgg aagggacaaa actgcgtgaa
    14101 aatgtggggc aaattttagt tcctgtacgc aaatcttata tcatccgtga tggaaaaaag
    14161 gtagagagag aaaaaaaatt atttaccagt tatgtgataa tagaagctga tctcacacct
    14221 gagttacgca cctatatttt aaatattgcg ggagttacga aatttctcgg acagagtaaa
    14281 acccataaag atcctattcc attaggtgaa gaagaaataa atagattact gggaatatca
    14341 gatagagaaa aagaaccagg aaaagctttt aattttatgc ctggagatat ggtaaaagtt
    14401 accagtggac ccttcactga ttttgaaggt gtaatacaaa aagttgcaga tgatggaact
    14461 aaggtagtaa tagaagtaac cgtttttgga cgcaaaactc ccgtagaact aaattctgag
    14521 caaattgaat taataaagaa ataagcaacg aggaatataa tggcaaaacc aaaagatgta
    14581 atggctattg ttaaacttca acttcctgct ggaaaagcta ctcctgctcc acctgtagga
    14641 ccagcattag gtcaggtggg agtaaatatt ccagaatttt gccgtcaatt taacgaaaag
    14701 acagctgatc aacctggaat ggttttcccg gttgtgattt atgtagcaaa aaataaatct
    14761 tttacttttg aaataaaaac tccacctgct tccgtattaa ttaaaaaaga agcaggctta
    14821 gcaaaaggtt ctgctacacc taataaggta aaagttggaa aaataagcaa tgaacaattg
    14881 cggtccattg ctcaattaaa gatgcaagat atgaattgta atacccttga atctgctatg
    14941 agtatgatcg ctggcactgc aaggagtatg ggtgtggtca ttgaagaatg atgaaaccca
    15001 cctatgcagg agataatatt cataaggaga atcaatgaaa catagtaaac ggtataaggc
    15061 tgcctatgca caatacgacc gccgtaaacg ttatgactta gatgaagcta taaatatact
    15121 caaatctctt ccctctccga aatttgatga aactgttgag cttcatttca atcttggcgt
    15181 tgacccacgt aaagcagatc agcagatcag gaattctatt gttctacctc atggaacagg
    15241 taagaatccc agagtgcttg tctttgctga aggtgaaaag gcggaagaag ctaaaaaggc
    15301 aggagccgat tatgttggtt cggatgattt gatagaaaaa attatgaatg gatggtttga
    15361 ctttgatatc gtaatcacca caccaacaat gatgggacgc ataggcaaat tgggcagggt
    15421 tttaggacca agaggtctta tgcctaatcc caaggttggc actgtaacca tggacattgc
    15481 aaaggcagtg caagaagcta aaagcggcaa aattacctac agagtagaca aattcggcaa
    15541 tttacatatt attgctggaa aattgagttt tactccggaa caacttaaag aaaacattaa
    15601 aactattatt gccgctgtat taaaagaccg tcctacatca ctgaagggcg tttatattaa
    15661 gagcattact ctctgcacta caatgggtcc tggtattaaa ctaaatgtcg caagcgctgt
    15721 tcaagaagcg agaagctgaa ggagctaaaa tgattcaaca caaaaaacaa aacacagtag
    15781 aaaatttaaa agaaagatta gcaggcgcta aagcgattat actggtagat tacaagggaa
    15841 tcaatgtgga acaggtaaat aagctacgta atctgtttag agaagcgcaa gtggattatc
    15901 tggtccagaa aaatacactg attaagatag cactgaatga gctgggtata aatgacctgg
    15961 atgtttatct tcaaggtccc actgcagtgg ctatcagtaa aaatgatgag gtcgctcccg
    16021 ccaaggtcct tgtcaaattt gtcaaagatg tgatggaaga agcagacttc ccaaaattta
    16081 aaggtggatt gatttcaggg aaagttatga atcaatccga gcttcaagaa attgcccaat
    16141 tgccttcacg agaagaattg ctggcaagat taatgggaag tatgaaagct ccactcagca
    16201 actttgtaaa tgtttcgcaa ggcatcattc gcaaatttgt ctatgcagtt gatgctttag
    16261 caaaacaaaa ggcgagctaa ataaaaatca tccctggagg aaaaatgtcc gataaaacaa
    16321 accaagttat tgatctgatt tccgagatga ccgtgcttga actctctgaa cttgtcaaag
    16381 aaatggaaga aagatttgga gtatctgccg ctgctccaat gactgttgca gctgcccctg
    16441 ctgctggaac aactgaaact caggaagaaa aaactgaatt tgatgtggtt ctgaacagtg
    16501 caggtgaaaa gaaaatcaat gttatcaagg tagttcgtga gattacaaag ctggggctaa
    16561 aggaagcaaa agacctggta gatggagctc ctaaagtagt cgcagagaaa atcagtaaag
    16621 aagaagctga atctatcaaa aagaagttag aagaagtcgg tgcctctgtc gaaatcaaat
    16681 agagtagtgg cgcaatatct tgttaatgtc caaaaaaagt cctacgggac tttttttgga
    16741 ccaattgtgc ttattactct cctctttcgt atattatctt gtgaacttag ggcaaattca
    16801 cgggcatttt attaggtcat aagcaataat taatatacta tgtttaacaa tcaaaaagga
    16861 gtgagctttt gagaaagatc aagagttatt ccagaatttc cgaaagattt gccgaattgg
    16921 gaatctcgga agttgaaata ccaaatctac ttgctatgca agtggattct ttcaatgact
    16981 tcttgcaaaa ggatgtccat ccaaaaagaa gagaaaaaag aggtttgcag gaagtttttg
    17041 agtctgtctt tcctattgaa gataacaagg gtaacttctt gcttgaattt gaagaataca
    17101 atgtgttaca agaaaaatac tctattgatg aatgtcggga aagaaattta acctatcagg
    17161 ctcccctgaa ggctaaattg agacttagca tttatgaaca aaaagaagga acacgcgaac
    17221 ataaggatac cattgaacag gatgttttcc tgggggaaat acctttaatc actgaacagg
    17281 gtacctttat cattaatggt gcagaaagag tggttatctc gcagttacaa cgttctccag
    17341 gagtctactt ctccgaagaa aaacatgcca gtggcaaaac tctttatgcc gccaaagtta
    17401 ttccctatac cggttcctgg ttagattttg atattgatat ccatgatgtt atgttcgttc
    17461 atattgataa aagaagaaaa ttaccagtaa ccaccttact tcgtgctatc ggaatatcta
    17521 ctaatgaaga attgagaaaa gtattctaca aaaccaagac cttgaaatta aaagaggcta
    17581 agggctgcta tctattcaat aatattgtgg tggaaggaaa taaagaaccg gttgcttttg
    17641 ctaatgaaca gattacggat actttaataa aacagctgga acagtttaaa attaaaaccg
    17701 tagaggtaat agacccggat tatgaaatag cacgtaaagt cctggaaaat actattgcta
    17761 aggaccctac aaataatcag gaagaagctc ttaagaagat ttatacccta atgagaccag
    17821 gtgaagaagc acccttagag gcagcaaaaa atctggtgga gagaatgttc tttaatgaga
    17881 aacgctataa tcttgctgaa gtgggtcggt ataagataaa cgaacgctta ggaattaatg
    17941 tagaccccaa gtgtttaact ctcacagtag aagattttgt ccatatcttc aaaactctgg
    18001 taaatatcta caatgataaa gatgatattg atgacataga tgacctctct aatcgtagag
    18061 tgcgaactgt tggtgagtta ttacaagacc aatataaaat cggtctatct aatgtagctc
    18121 gtattatcca ggaaagaatg tctatctcta atattgatga agtaactatc catgacctgg
    18181 ttaatagtaa tgcactggtt gctgcagtac aatcattttt cctaaccggt caattatctc
    18241 aatttatgga acaaactaat cctcttgccg ctctcagaca caaaagagcc ttatcagctt
    18301 taggaccagg cggattaact cgtgaacggg cagggtttga agtacgagat gtgcatcctt
    18361 ctcactatgg aagagtatgt cctattgaga ctcctgaagg acctaatatt ggattgattg
    18421 tttcaccaac tatctattcc aggattaatc ggttcggttt catagaaacc ccctatagaa
    18481 aggtggtaga tggatatgtt accgatgaat atgaatattt ggatgcttcg caagaagcaa
    18541 aatatattat tgctcaatgt aatgtgaatc ttgatgaaaa gagacatatt acagacgaat
    18601 ctgtgttcgc tcgtcataaa ggcgatttta tctctgttga ccctaaaaat gttcagttta
    18661 tggatgtttc acctcaacag atggtatctg tttccgctgc tcttattcct ttcttggaac
    18721 atgatgatgc taatcgtgct ttaatgggct caaacatgca acggcaagca gtgcctttga
    18781 taaatcctgt agcaccaatt gttggaacgg gtatggaaaa aatcgctact atggatactt
    18841 ccaacattgc tgtggcacct tatgatggat tggtcactaa tgtaacttct tcatatattg
    18901 aaataaaacc cctccacgaa gaagatgaag ctctatatct gggattgggt aatagaattg
    18961 aattaaagaa gtttgtgcga actaatcagg atacctgcaa taatcaaaga cctattgttc
    19021 gcattggaga tacagtccgt aagggacaac ccatctctga tggtgcttgc gtggaagata
    19081 atcgtttagc actgggcact aatatgatgg tagcttttct gccttggtac ggttataact
    19141 atgaggatgc tatcatcctg agtgaaaaag ttgctcgtga tgacattctt acttctattt
    19201 acattgaaga aatggaagtc ttggtgcgta atgtgaaaaa tggtaaagaa gaattagctt
    19261 atgatattcc caatgttcct gcttatgctt tgcgtaatct ggataaaaca ggaattatcc
    19321 gtgtgggctc agtgattcat gcaggtgata ttatagttgg caaaataaca cccaagagtg
    19381 tggaaataga gccatctcca gaagaaaacc tgatgcgtgc tctttttgga gatagagcag
    19441 gagatttcac caatagctcc ttaaaagcta aaccgggtat ggaaggtgtg gtgattgatg
    19501 ttaaagtctt ctctcgttta gaagaaggca ttgaagagga agaagaccag gaagagaaga
    19561 aagccaaact tagaaatgac ctcaacctgc gcagacaaaa gattgaagaa ttcaaaaaag
    19621 aaaaactctc cgctgcattg ttaggtgagg ttgcacatac tatctgggac gaaaaaacca
    19681 atgtctattt cattgcaccg ggaaaaaaga taacaaaaag tgatcttgct cgtatcaatt
    19741 tcaagaagtt ggatttggat gcgcagctcg tggaagaccc ggaaaaaaat gagaaaatat
    19801 accaagacat catcttgaaa ataaaacaat caatggaaca gagtgagaac atttatcgca
    19861 aatccttaga aagacttaaa catggtgatg aacttcaata tggagttcgc aaaatggtta
    19921 aagtctatgt ggcaaaaaag cgcaaaattg aggtaggcga taaaatggct ggacggcatg
    19981 gtaataaagg tgttatctct attattgcac ctattgaaga tatgcccttc ttagaagatg
    20041 gaactcctgt tgatattatc ttgaatcctt taggtgtccc ttcgcgtatg aatattggac
    20101 aaattatgga aacccacctt ggtatggcgg ttaaaacctt aggatacgag attgaaaccc
    20161 ctatctttga cggagcaacg gtccaggata ttgaagctgc tttacaagaa gctggacttc
    20221 caaccgatgg taaacagagg ttatatgatg gtaagactgg agaaccatat aaagaacgag
    20281 tgacagttgg tattatctat atgatgaaat tgaatcatct ggtagctgat aagatgcatg
    20341 ctcgttcaac tggtccctat tctttgataa cacaacagcc tctgggagga aaagcgcaac
    20401 atggcggtca acgattggga gaaatggaag tatgggctct ggaagcttat ggtgctgcgc
    20461 ataatctgga agaaatgcta accattaaat ccgatgatgt ggatggcaga aatgaagctt
    20521 ttaaagctat caccagggga gaaaatcccc ctccttcggg tgtcccggaa tcttttaatg
    20581 tgctcgtgag tgaactaaaa tccctcggat ttgatattga gttcctcaaa gaacacgaat
    20641 tgaatgagga gaagtaaaaa tgttaaaaga taacccacat gaaataaaac caaacgactt
    20701 tgactttgtg cgtattaaaa tcgcttctcc agaaactatt attaatgaat ggtctcatgg
    20761 tgaagtaacc aaaccggata cgcttaatta tagaaccctt aaaccagaaa aagatggact
    20821 attctgtgaa aggatttttg gacccgaaag agattatgaa tgtgcctgcg gaaaatataa
    20881 aaagaaacgc tttcagaatg tcgtttgtga ccgttgcggt gttttagtaa ccacttcccg
    20941 agtacgcaga actcggatgg gacatatctc cctggctgtt cctattgctc atatctggtt
    21001 tgttaaaagt acgcccagta aaattggtac tttactggat atgaccgtta aggatttgga
    21061 acgagtaatc tactacgaat catatatagt ggtagaccct ggtgatagtc cttatgaaaa
    21121 atatgaactt attgatattg atgaatattc cgaaataaag gataaagtag gtgataattt
    21181 cctggctatg atgggggctg aagcaattcg tgagctcctc tccagattgg atttaaataa
    21241 tgaagcttta aatatccgct ctcgcttaaa aatggaaaaa tctactttat acaagcagaa
    21301 attaattaat aagctaaaga ttgtggatgc tttcctaaaa tccggtaata aaccagaaga
    21361 tatgattcta cttgcgcttc ctgttcttcc tccaactctt agacctctgg tgcccttaga
    21421 aggtggaaga tttgctaccg ctgattttaa tgagctctat cgccgagtta tcactcgtaa
    21481 taatcggtta aaatatctta tggaaatccg ctcacctgag gtcattcttc gtaatgaaaa
    21541 acgtatgtta caggaagcag ttgatgcttt aattgataac tctcgtaagg ctcgtcccat
    21601 aaaaggaaga ggaaatcgtc ctctcaaatc tttgagtgac caattaaagg gtaaacaggg
    21661 cagatttcgt caaaatcttc tgggtaaacg agtggattat tctggacgtt ccgttataac
    21721 tgttggtcct gagttaaaac tttatgaatg cggtttacct aaagatatgg cagtggaact
    21781 atttaaaccc tatttaattg aaagactgca aaagttaggt gaagctgata aagtaaaaaa
    21841 tgcaaagaaa cttatagaaa agaaacaacc agaaatctgg tctatattgg aagaagtaat
    21901 caaagattat cctgtccttt taaatcgtgc accaactctt catcgtttag gtattgaagc
    21961 ttttatgcct gttctcacag ataataaagc tattcaattg catcctatgg tctgtgtgcc
    22021 ttttaatgcg gactttgatg gagaccagat gggtgtgtat atacctcttt cctatgaagc
    22081 acagattgaa gccagaactt tgatgctttc tacccgcaat ctgcttctgc cttcaaatgg
    22141 acgcttagct atggcagcaa atcaggatat cgtcttgggt tgttattatc tcacgatgga
    22201 agagtttcct gaaccagaag atattaccac cttgcgtcat ttttatagca ccgacgaagt
    22261 aattgctgcc tttgaagctg aagaacaact tttctctgtt aaaggtgatg tggaagagga
    22321 aaggtcttta aatctccaca cctggattag agtgaagata aagaataatt atatcgttac
    22381 tactgttggt agagttatct ttaaccaaat aatcccggaa gaagtaggct ttcaaaacag
    22441 tgtatttgat aaaggtaaaa tcaacgacct ggctatgctt tgctatgatg ccgtgggaca
    22501 atggaaaacc gcacaattcc tggataatct caaagaactt ggctttcatt attcaaccca
    22561 tgctggagta actttcagtc tcagtgacat cgttgttcca aagcgaaaag atgaaattat
    22621 cgctgaagct gaagaggaag ttaaaaaggt tatggacctc catatgaaag gtgggattac
    22681 tgaaaatgaa cgctttggca gaattgtaga tttatggaaa aagactactg ttcgtgttac
    22741 cgatgaaatg atggaagaat tagccaaaaa tcgtaatggc ttaaactcca tttatatgat
    22801 gtataaatcc ggagcccgtg gtagtaaaga ccagattaaa caggttggcg gaatgcgcgg
    22861 tctgatggat aaacctgcta aaattggttc taccggtggt gtggatgtaa tagaaactcc
    22921 cattaaatct aatttccgtg atggattgac tgtgctggaa tattttattt ccactcatgg
    22981 tgctcgtaaa ggactcgctg acactgcttt gaaaacagcc gatgctggat atctaacccg
    23041 ccgtttagtt gatgtggcac aaaatgctat tattactata gatgattgtg gaactaccaa
    23101 gggcgtagat atgtccgtct tgaaagaagg aaatgaagtt gtcgctactc tggcagatag
    23161 aattcaagga agagtcgctc tggaagatgt aattgacccg gtaacgggaa atatcctggt
    23221 ccatagcgga gaagaaatat ccaataaaat ggcacgcact atccagaatc atggagttat
    23281 taaagtaaag gtgcgctctg ttctcacttg tgaagccgaa catggaatct gtgctaagtg
    23341 ttatggtcgt aatttagcaa ctcagaaacc tgcagttatc ggagaccccg tgggtattat
    23401 agcggcacaa agtattggag aacccggaac tcagcttact cttagaactt tccatattgg
    23461 tggtgcagca tcaactgcta ctgaattggc tgaagttgtc tctgcacacg acggaatcgt
    23521 taaatttgac cgtatgaata ctgtgaccaa tgtgaataat cagcttattt ctgtcagcca
    23581 cctgggtaag atattaatct gtgatgagaa tgaccccagt aaaattctgg aagagtatag
    23641 agtggagtat ggtgctacta tccatgttcg ggacggacaa aagattgcaa tgaataccaa
    23701 actgatgagc tgggatcaat tcaataatcc tttgatttct actgctaagg gtcaattgca
    23761 ctatgaaaac tttattaaag atataaccta taaagaagaa tttaacgata taaccttctc
    23821 ccgagatatc actattatag aatcaaaaga ccgtaaaaaa caacctcaat tcaggattgt
    23881 tagtgaagat ggaacttctg tccaagtacc attacctaca ggacttattg tcaaagtgga
    23941 agacggaagc tttgtccatc caggtgatat tttaggacag acctcacgta tgactattaa
    24001 acagcgtgat ataactggtg gacttcctcg tgttcaagat ttatttgaag cacgagtccc
    24061 caaggataaa gcttgtatct ctgatattga tgggatagtt accatcggtg gtttaaagaa
    24121 aaatggacgt gaaatatttg ttactcctcc caatggatta atggctccag atgatggaaa
    24181 ggtgattgtc cagtctgatg aatatgttaa tagaattatt cttctaaccg ataaaactgt
    24241 ctatgcgaat aatgatggta aagtgagcat tattgaagag aataaaaaga aaaaagtagc
    24301 tattgccaaa cggaatcaga aacctcaagt ttatgaaatt cctcgtggat tacatattat
    24361 tgttaatgaa ggtgataatg ttaaggtagg agctcctctt tgcggtaaga tactggaaat
    24421 ccctcctcta caggaaagca ttgttagcac tggtgataca gttaaaaaag gacaaccctt
    24481 aaccggtaga aaatatatca ttccttcagg aaaacggatt atcgttcacc agggtgacta
    24541 tgttgaaagt ggtgatgctt tatctgatgg acctcttgac cctcatgata tgttagtaaa
    24601 aggtgttatt gaagctcaga tgatgatatt aaatgaagtt caagaaatct accgcaaaca
    24661 gggtgtgaag attgacgata aacatattag tgtaatcata cggcaaatgt tcaaaaaagt
    24721 gcgcatcact gatagcggaa gcacctcctt ccttgaaggt gatatcgtag ataagacgct
    24781 ggtagaaaag gaaaataaag aagtccagaa atatggaaaa actcctgcgc aatttgaaca
    24841 attgcttttg ggtattacca aggtttcttt actaaccgat agctggttat ctgctgcttc
    24901 tttccaagaa actacaaatg tgttgacaaa agcagctata gaaggaagtg ttgataaatt
    24961 agaaggctta aaagaaagtg ttattatcgg tcatagaatt cctgtgggaa ctggaactaa
    25021 aatctataac aaaatgattg aagaagcagt gaactctggt gctacggtag cagaaattat
    25081 tgagcgattc gctcacccaa aaaaggaaga agaaagtgaa gatatatata acttctaata
    25141 ataaataagg aggtacttag tgccaaccat taatcaatta gtccgtaatg gaagaacaaa
    25201 gattgaaaag aaaaagaaaa atagggcgct tatgggatgt ccgcaaaggc gcggagtttg
    25261 tactcgtgtt tatacgacaa cgcccaaaaa acctaattca gcgctgcgta aagtagctcg
    25321 tgttcgtcta actaacggtt ttgaagtcac tgcctatata ggtggagaag gacataatct
    25381 acaggaacat agcatagtat tagtcagggg aggtcgtgtt aaagacctac ctggtgttcg
    25441 ttatcatatt gttcgtggtg ctctggatgc tgccggagta gaaaaaagag ttaattctcg
    25501 ttctaaatac gggaccaaac gtcctaaaaa agcttaataa gatggagatt taactatgcc
    25561 aagaaagaaa aaagcagtcg ttcgtgaagt actccctgat cctatatatc aggatgttgt
    25621 cctctccaag tttataaatt gcgttatgaa aaaaggcaaa aaaagcatcg ctgaaaaaat
    25681 cgtttatggc gcctttaata ttattgagga aaaaactaaa cagcccccac tggaagtatt
    25741 caaaactgca ataaacaatg tccgtcctca acttaaagtt gtctcacgca gagttggtgg
    25801 ttcaaactat caaattccta tggaagtttc tgaaaagaac gggcaggcac ttgcctttcg
    25861 ctggattata acttatgctc gtgccagaaa tgaaaaaagt atggatgaaa aattagcagc
    25921 tgaacttatt gctgcttcca aaaatgaagg tgcctcaatt aaaaaacgtg aggacactca
    25981 taaaatggct gaagcaaaca aagcttttgc ccatctgaga ttctaagata ccctattaaa
    26041 gatgttaatt aaaaccctta aagtattgat aagctcatgc tttaagggtt ttgttatttt
    26101 tataatatga taagcttccc cattgatata atcttaaaat attccacagt gtggaatgcc
    26161 acttgcttta tatatcaata aattattttt tgcgaggacc aatgaataca tctatgtttt
    26221 cccgattaca tataattcta ttagctcttt taatattttc tctttctctt ccggtttttg
    26281 ctgaatttgt ccaaccccaa gaggcaaaaa ttatagctca aacctggcta aatcttatag
    26341 atggaaagaa ctattccgag ttaaatattc agcaagtctt ggaatatagg gataacaatt
    26401 ttactctatc ccaatccaaa tatgagctca cagatgaaaa tcttcctcct ctttatttga
    26461 ttctatttca taacaacggc ttcgtattga tttctgctga agataattct atcccggttc
    26521 tggcttattc ttctgattcc agttgtaata tcaattccta tccaccagct tttctggaat
    26581 gggtggaaaa ctatgcagtt caaatcagac aaataagaga tgaaaaaact gttattcctg
    26641 agaatgcaca actctggcag agtttattta gaggtaatct accagctaat ttcagacaag
    26701 accgtgctgt acgtcctctt attgttacta attgggatca gggttggccc tataatgaac
    26761 tttgtcctgc tgacccaaat ggaccaggag gtcatgttta tgcaggttgt gtagctactg
    26821 ctatgggtat ggtaatgaaa tattggagtc atccaaccac tggtgtgggt tctcattcct
    26881 attattgccc aggttacgga tatcaatctg ccaattttgg agctacaact tatttatggg
    26941 atgaaatgcc aaactccata tctacttcct gtatccctat tgctactttg ctttatcatt
    27001 gtggagtggc tgttaatatg ggctattctg ttgacggttc tggagctcaa agcaccgatg
    27061 cagctaatgc tctggtcaat tatttccgct atccaaatgc tgttttacag aataaaatgg
    27121 gaatgtccga tactcaatgg aataatctct tgcaaactca attagataat ggttgtccta
    27181 tgtattattc cggttctggt tcaggaggag gacatgcttt tgtaattgat ggatatcaaa
    27241 caccaggata ttatcatttt aatttcggtt ggagtggaag ttcaaacgga tattattata
    27301 taaataatat aaatcccgga ggatatgatt ttaatagctg gaattccgtt atcgctaata
    27361 gtattcctca aaattataat atcaaccagg ttagaataaa tatgaatgcc tttggtgcaa
    27421 ctgtgggaac caattttaat ttaactgttt ccactttccc tgttctcggt tcttggaatg
    27481 tgaatcattt tgaatttaca cttatttacg acagcgataa cataactttt aatggtgctt
    27541 ccattgctaa cggtattgcc tcggatggaa acctttctgt tagtgaaacg gaaccaggtt
    27601 atctacaagt tagctggaat ggcaattcta atattatcgg aagtggagat ttaattactt
    27661 tttccttccg cccactggat acgggtcaat atctttttga tatggtagga atgacttata
    27721 ataatactcc cataactaca acctctttta taatggttga tgttgttccc ccagtgacaa
    27781 atcttacaga aagtgctatt actatgttaa atgttatgca tttagcttat aatcagattg
    27841 gaaccactga aatccgtacc acttatctgc ttccttcctg gaatgtaacc cactatcaat
    27901 ttgatttaaa ctatgacgca agtaaattgc aatttatggg aatagatacc gctggaacta
    27961 ttagcgctgg ttgcgaacct tctgtggtaa taaataatcc cggttctata tccattagct
    28021 gtgatagtaa taatcgtttc actggtgatg gagccctact aaagttccat tttaaagcca
    28081 taggtaatgg tccaactgtt agtattactc aggtttcacc tgctaacttc ttctacaatg
    28141 atactcaaat ctttaatctt ggttccgcta actttatctt atctgcttat actgcaaatg
    28201 atgatgaatt agctcctgaa attacttctc tacaaatttt ccctaatcct ttcattaaca
    28261 gcacccagat taaattaaat agtaaatcca gtgaaccggt acaatttact atatataatc
    28321 ttaaaggtca gaaagttagc tcgttagtta ttactgattc tccaaaaact tttacctgga
    28381 ttcccaagga tatgatgggt aaaccactac ctacaggagt ttatttgctt tcttgggaac
    28441 agggcaaaga aaaaggaacc gcaaaactct tatattttaa ataaaccttc acctacacct
    28501 taggca
//
LOCUS       DAORZF010000017        27612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_1483_length_27612_cov_406.994883, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000017 DAORZF010000000
VERSION     DAORZF010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 27612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27612
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1483_length_27612_cov_406.994883"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            232..1173
                     /locus_tag="PK015_03250"
     CDS             232..1173
                     /locus_tag="PK015_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HOL33520.1"
                     /translation="MAGTLIGILVAIFIIVLISRGIIVVRQAEVVIVERLGKYYRTLD
                     SGIHIIIPIIDKIRPIHWRYTREDYRGNMVVTQRLENRIDLRETVYDFPRQNVITKDN
                     VSITINALLYFQVTDPYKAVYEISNLPEAIEKLTQTSLRNIIGELTLDYTLTSRDIIN
                     AKLRDILDEATDKWGVKVNRVELQEILPPEEIRTAMEKEMRAERDKRAKILQADGERE
                     YQVRVAEGEKLAKIARADGEAQAKLLVADAERKSIELIAEAVKNTGTDPAQYQIALKY
                     ITAFQEIVKQGDKTVVVPYEATAMLGSLKTMEELFHK"
     gene            1192..2469
                     /gene="eno"
                     /locus_tag="PK015_03255"
     CDS             1192..2469
                     /gene="eno"
                     /locus_tag="PK015_03255"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOL33521.1"
                     /translation="MSEIVYIKGREILDSRGNPTVEADVMLDSGVFTRAAVPSGASTG
                     EREAIELRDGDKKRYDGKGVLKAVENINSIIAPALLNIEATHQEEIDSVMLNLDGTPN
                     KSKLGANAILAVSMAVARASAIELDLPLYRYLGGVGAKTLPVPMSNILNGGVHADNNI
                     DIQEFMIMPLGANSFQQALQMNAEIFHALKGILKKQGLFTGVGDEGGFAPNLKDNEEA
                     LKVIMEAIVAAGYQPGKDVFIALDVAASSFWKDGKYLFEGKTFSSSDMIDYYINLVQK
                     YPIISLEDGLAENDWENWIILTQKLGNKIQLVGDDLFVTNPAIIKRGIAENAANSVLI
                     KLNQIGTVSETIEAIQTAHKAGWTSVVSHRSGETCDTFIADLAVAMNTGMIKTGSLSR
                     SERIEKYNQLLRIEEELGSAAIYPGRAVIKQLS"
     gene            complement(2999..5197)
                     /locus_tag="PK015_03260"
     CDS             complement(2999..5197)
                     /locus_tag="PK015_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HOL33522.1"
                     /translation="MNKYLLLLLLLPVMIFAQPWQQDNAIFNPSGIPSLPFSQPRFVD
                     LDADGDMDFFLGNTNSAPLYICNIGTATSPHFVIGDNLLANISFIDAELAVCADMNAD
                     GKLDLITGGYTGLHLFLNTGTIQSPVYTEQLGYFSALEVGHNPVPDVADVDGDGDLDL
                     VVGLSEDGAVRVYFNTGTASNGIFLEESMQLIGDVGLYAYPVFCDLDNDGDQDILCGR
                     DAHGFVYYQNNGTISSPLWENNSALFSGLGNSTYWNSPDLVDLDGDGDFDLIFGTADG
                     PLKYYVNNGTPSSPQWQENTNLFGGVIDAGGASSPVLFDFDGDGDLDLICGTQLGDIK
                     FYKNIGTPYAPAWQEDSSYFSSIDHSIYSAITLGDVDADGLPDAIVGDLSGNLYFHKN
                     TGTGFIQVNNVLPPISVGGWSVPRLIDMDSDGDLDLAVGNEAGNIYYYQNNGSPELPQ
                     WTLVSGFFGNIDVGSDCSPTFGDLDENGRLDLLAGDLFGSLHCYLYQNHNWVNNTELF
                     AGIETNQNAAPALADLDHDGDLDLVLGDYDGTFKFYRNLKYSPAVLNPPLNLLANTDE
                     PVTISWEEPQGSTSPLEHYNIYLDNEFVDSTTFTFYSFFNWDFTVAHIVKVTAQYVAG
                     ESVPAIIEIPATPISDEYQSIGIISIYPNPVTDITNINLTLKSEDKATVSIYNLKGQQ
                     IIGWEVVSKGNHNLIWDGKDKQGKPVANGIYLLNVKTPQNTITRKISLIK"
     gene            complement(5316..6689)
                     /locus_tag="PK015_03265"
     CDS             complement(5316..6689)
                     /locus_tag="PK015_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4080 domain-containing protein"
                     /protein_id="HOL33523.1"
                     /translation="MLSNQGNKKICFIAVNSAWYQSNPALYYLRNALEGLPYHIEIYE
                     FIADEPLFDVLSTIVSSKADILCFSAYIWNQLYLKLLIPEVKKLLPEAKIVIGGPSAL
                     CEDYKLNDSDFIVKGAGEGAFRYLAENNFSLEGGIYEVPAPSLNTLPFLYKEEDRAAL
                     EGKLVYYECSRGCPFHCIYCLSARDKRNELRFNPESSEDRTRLYNEMEQLLTLKPRTV
                     KFVDRCFNSNPDLAHLIWDYVIQMQADCEFHFEIFPELLKEEDLTLLAKAPAHRIRLE
                     AGVQTVNPDIAKACGRMSNWEKAKKSLAFLCENTQIQLHTDLLAGLPGESMASVLHSL
                     DELASIFPDEIQLGSLKILPDTPMYDLAKQKHYLWLDTPLWQVLRTDTLSFAELSTLN
                     ALGKILNLYWNKKEFNSEWRTLLDSGKKASQLALSLLAYHQENHITLHSISRQERYNI
                     FAMVSNL"
     gene            6905..7810
                     /locus_tag="PK015_03270"
     CDS             6905..7810
                     /locus_tag="PK015_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOL33524.1"
                     /translation="MLLADFLEPSTIVFEQRILTREEVYKDIITRICQLRSDIVPNCG
                     ETLLELILEREKEAPMVYPSGIAIPHIRLDGLADTLIGMTFLQNPLNYDGIKVDWIVM
                     IFTDKTSSKIYLNIVAALLKISEDEEMMTQLRSFNDGYAVVNYLRQCRIKVGQELCVS
                     DLMVKNPICVTPDTLLSKVDSLMNVHNISCIPVVDNEGRYLGEINILNILKVGMPDYL
                     MMIDNVAFLRSYEPLESLFEKEEIVKAGEIMQKNTRVLSSDASIVETVYHMLKDNKRY
                     FCVVDNGRLVGIITAMDIFRKVIKA"
     gene            7807..9096
                     /locus_tag="PK015_03275"
     CDS             7807..9096
                     /locus_tag="PK015_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HOL33525.1"
                     /translation="MSATVSMLIAVGIFIGTYILIITEVINKMLASLIGAFLLILTGI
                     LSQDIALRAIDWNVIFFLIGMMLVIAVLRRTGIFMYIAIKTAKLARGNPLLIMMLMFV
                     VTAFSSAFLGSVTSIMILIPIILLICNELKITPVPYIITMVVASNMGGAATMIGDPPN
                     IIIGSATDYDFLDFIFNLTPAIILATIACLGLMWVMYHGKLKVSRENRARMMSYKEEG
                     LIKDKRMLKISLITVAVMLVLLALESTLKIGTASISMSAGLFLILVGQRNDIEHIMVH
                     EVDWVTLFFFVGLFIIVEGLVQTGFISLLAHKVLELTHNQARPTSMAILWLSGILSAF
                     IDNVPFVATMIPLIKQIGTVISQPNLMDPIWWSMSLGTCLGGNGTLIGASSNVVAIGI
                     TKKNGINISFMEFTKVSVAYTILSLVISSVYILIRYF"
     gene            complement(9114..9791)
                     /locus_tag="PK015_03280"
     CDS             complement(9114..9791)
                     /locus_tag="PK015_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HOL33526.1"
                     /translation="MCGRFAQVIKYDQLQRLEKELKLNRESDQLTINFNVAPTQPVGA
                     AVSQDSGRYIGTFRWGLIPSWSQELPKFDLINIRSDSLISKPSFKRGLQYRRCLIPAN
                     GFYEWRKTDKQPFFIHSAQDDFIYMAGIYDVWQSPEGSYVPSLAIITTDANQVMLPIH
                     NRMPVLLFADDRLTWLEHTIKDINKFIPLLKAPPDDLISMYPVSRVVNNPQNNSEECI
                     RPIDITE"
     gene            9887..10537
                     /gene="nth"
                     /locus_tag="PK015_03285"
     CDS             9887..10537
                     /gene="nth"
                     /locus_tag="PK015_03285"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOL33527.1"
                     /translation="MNNFDIDTVMAELEKHFTRVRTPVVDQIQIQTKDPFKVLVTTIL
                     SARTKDETTSQAAKKLFEKVKNIDDLEKISAEELDSIIHQVGFHNAKTKYLKQLPSVL
                     KEKFGGKIPSEIDELLQLPGVGRKTANLVRAIAFSLPAICVDVHVHRICNRWGYVQTK
                     TPYETEMELRKILPERYWLTFNSYVVAFGQNLCTPRKPKCNQCPIYNQCARIGVKE"
     gene            complement(10543..>12138)
                     /locus_tag="PK015_03290"
     CDS             complement(10543..>12138)
                     /locus_tag="PK015_03290"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOL33528.1"
                     /translation="LHWMDEQSRNFFHILGKSDIKDTGILATTRYIENDRLPILNMDN
                     FSFYRFDLAPLSKNYSWQLYKSMMNLDDVPADLKQEILQKDTGNPFIIEQMASYLLES
                     GVLENSADYLNILGKDNSFDVNDIIGFRIDRLPFSVRECIYNASVLGMKFNLKVLSQM
                     LKRDIRQDLLIGTQNRIWKSLERDFYIFTHILLQEAAYNRMFSDKLKSLHLKAAQSME
                     EVFAQNISEYSEEIAKHYEKAGEIIKASDYYDKAGDYYWENSYFELAEKCFLKAVELA
                     EKSFGTDNMNYGENVFHLALFYHYMQRMEEAEKLYDTVMNIALATYGEDSLKLSPYIN
                     NLGRFYKDVGRYEESERLLLQSLELEKKHEPDSSNVADRINNLGHLYSLQNLNDKATE
                     MYQQAYDIMNRNYDPYHFFTATVCNNLGMSFAESGKIEEAEKLLQRAVEIGKKIYGEN
                     HPQIVIYFKNLASIFMDQQRWNEAEALLHKCLTIDRKYFGKYHNATLYIVKALEKFYD
                     KIGNKRKARYYSKWLQEAKELSQ"
     assembly_gap    12139..12238
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12239..13635)
                     /locus_tag="PK015_03295"
     CDS             complement(<12239..13635)
                     /locus_tag="PK015_03295"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOL33529.1"
                     /translation="MIRYIPPFILSQYEKDNVQGKMQAFVLLGDIIGFTTINDELQKK
                     GKEGAEEVSHLITNIFSEPLNIIEKYGGFLSNFAGDAFYAIFPGAQPENIMAALHYIQ
                     SFLRNQYQKQRLLNKSPVKMRIRIGYGTVQWMIFSNDIQMEYVFFGDAIEEVQSLNKG
                     QTTCRYSTSALQKLISSRLVDDKGKISRDFNPEKVKENIINYPYQKETEYKFLHYRLH
                     NIHPENEIRTIISNYTRIPFTADLDICSIIANLEIFYQYSGSFVDKLSVTNNSLCILS
                     YYGMPRNEGKKLSQVFQLILERVHKYPFISQAISMGPCFTGFVGGEHIREYTALGHPV
                     NLAARLSGKSKKGEILTDITLKKELERNFDFEFVGNLSLKGLKKRIPCYKLKELQPSV
                     PELRTVFVGRQNELKSIQRIISKSIAEHHNYFIYIAGEAGIGKTRLATEVINSCQDKN
                     IRIFHIMCSITNSRP"
     gene            complement(13668..14171)
                     /locus_tag="PK015_03300"
     CDS             complement(13668..14171)
                     /locus_tag="PK015_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HOL33530.1"
                     /translation="MRVIKINLPLQPQQKKELKVRDKILLSGELYTARDQAHIRLTQL
                     IKEGKPLPFDLSNSALFYCGPAPIPPGKVCGAIGPTTSARMDKYTPLLLENGLRVMIG
                     KGERSKEVTEEISKYDAVYFICVGGISALLAQSVVSCETFCWKELDAEAIYRLVVKDF
                     PCYVAII"
     gene            complement(14197..15066)
                     /locus_tag="PK015_03305"
     CDS             complement(14197..15066)
                     /locus_tag="PK015_03305"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004885.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOL33531.1"
                     /translation="MESVRTIPKKQIYKAIIEAIGQIYCQSTPETKSLLNQALAIEED
                     DVAKDMLQAMLLNEELAAKEQLPICQDTGIVIVFADIGTETKIENGTLYSIVSEAVND
                     AWGKYYLRDSIAEEPLRYFSNPQKKSKYYSQPVILHLSEILGNKLELHISLKGGGAEN
                     CSTLKMFNPTATLTEIEDFIIDTVVSAGGKPCPPVFVGIGIGGDFEQCAILAKRALMI
                     PLPDSEKKPEYLQMEKRILNKINQKGKGVQGFGGKTTALKVNILSQPCHIASLPIAVN
                     IDCHAHRCISISV"
     gene            complement(15071..15955)
                     /locus_tag="PK015_03310"
     CDS             complement(15071..15955)
                     /locus_tag="PK015_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOL33532.1"
                     /translation="MNQQLLTDETAAKLKRFQLLAKTTVEGFLVGLHKSPYHGFSVEF
                     SDHREYNIGDPIKNIDWKIVAKTDRYYVKRFEEETNLRCYILLDHSRSMFYGSGENTK
                     IDYACRLAASLSWLMIGQKDAAGLITFNNEITKLLPPKAYRGYLPQIFRILAELEPKD
                     STHLLENLHKIADTIRKRSLIILISDLLDEPENIMDGLKHFKSHHHEVLVFHITDIQE
                     DNFKFHGETEFIDSETQEKITVNPWQIRPDYLLNYQEHIQKLKAGCHQYQIEYNPVST
                     ATPIEDLLLKYLLMRRKG"
     gene            complement(15952..16143)
                     /locus_tag="PK015_03315"
     CDS             complement(15952..16143)
                     /locus_tag="PK015_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33533.1"
                     /translation="MANNNKLQLGPINYIILGIAVILLIVGYFIMAGNEIVISPVILT
                     IAYGVLIPLGLLYKSKPKE"
     gene            complement(16162..16962)
                     /locus_tag="PK015_03320"
     CDS             complement(16162..16962)
                     /locus_tag="PK015_03320"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOL33534.1"
                     /translation="MMWIVKLFHTIWQMILLWSFICRSYFIRLFAKNPIKRRWLQIKN
                     MSNSSRKFLRAFHVKLRVKNQEVLQQLKDNNYLLVANHSSLLDIFLLGAVDNYVFITS
                     TEMSETPIIGNIIRLGGCLYTDRKNKLNLPQEIKRFTDVLKKGFKVVLFPEKPGTDGT
                     KVNEFRPSLFQTAIDAACTVVPVCIRYLKLDGKPINGKNRFVVFWSKSINYLKYYWNL
                     LAHKMEVEINFLEPIEFDPNRDRSELCQLTYEKVSNTFESYENCVAGS"
     gene            complement(17411..17796)
                     /gene="rnpB"
                     /locus_tag="PK015_03325"
     ncRNA           complement(17411..17796)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK015_03325"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(17937..18746)
                     /locus_tag="PK015_03330"
     CDS             complement(17937..18746)
                     /locus_tag="PK015_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOL33535.1"
                     /translation="MKTSNIIIIALAVLILNAGITLVLINQKEINAPASTTTIVDPSH
                     NQRHNEITSAVQAVEPAVVSVNVTKTQLVRPGFGFSIFDFFGDIPIQRQVVSIGSGVI
                     YDANGYIITNAHVIEGASEIKVVLPDKREFIANVVGVDNTYDVAKLKIDGNNFPYAKL
                     GTAKELLIGEWAIALGNPYGFMMSDPKPSVSVGVISALNRSFQPQDGHRYINMIQTDA
                     AINPGNSGGPLVNVKGEVIGINTFIVSGTGDNVGLGFAIPIDRVKSIIKIL"
     gene            complement(18755..19510)
                     /locus_tag="PK015_03335"
     CDS             complement(18755..19510)
                     /locus_tag="PK015_03335"
                     /inference="COORDINATES: protein motif:HMM:NF014078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HOL33536.1"
                     /translation="MKDQDLYLSVIKFPAFVSISLLIISAVFLIILFNVFAQPLLANW
                     VRTLFIILLAILIFIVLGWMITLFSTHTRLKPRWLQKLSKWMLFYIYYPLAKGVAHIT
                     FSSKKNLIESFLNFNNEIVLTDQKDISNTNILVLLPHCIQKEDCTIRITNDIIKCEEC
                     GGCDMAKIKKLITEQNVDAAVATGGFLERKMIEEKNPEVIVAVATAKELVNSLRDNWN
                     YPVYAVLNEQSITANEKNPVNISFIEFAIKQFK"
     gene            complement(19507..20481)
                     /gene="fmt"
                     /locus_tag="PK015_03340"
     CDS             complement(19507..20481)
                     /gene="fmt"
                     /locus_tag="PK015_03340"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOL33537.1"
                     /translation="MKIVFAGSSEFGIPALKKLKEKHQLLLIISQPDRPAGRNQRIKP
                     CPIARFVREQGWELYQPENINSPESVDKISKLYPELLVTASYASLIKKELRQIPTFGA
                     INLHPSLLPKYRGPTPIQTALLNGDHKTGTTIFRLTSRLDAGPILAQRELVILPEDNF
                     GSLKEKLANISAELLIEIIPAIKDNSLTERPQDENLASYTHKFSKKDFWINWDNKIKN
                     IFNQIRAFSPSPGARTSIHNTNLKILQAEIYDSSKNDKPGTIAGFIKNKGILVNCQDG
                     QLLITRIQAEGKKVMSAWAFQLGARLTAGESFVPFDKIFIENSYREEL"
     gene            complement(20498..21052)
                     /gene="def"
                     /locus_tag="PK015_03345"
     CDS             complement(20498..21052)
                     /gene="def"
                     /locus_tag="PK015_03345"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOL33538.1"
                     /translation="MSRKPKLLPIHIYGDKVLRLKSEEVQKIDENLLQFAEDLIATMY
                     QWDGVGLSAPQVGVSKRIIVIDPQIKDNPKKHSPMVMINPVIEESSGETETEEGCISI
                     PDIYAKVVRPSNITVSYTDLQNERHRINLSGYAAVIVQHEYDHLEGILFVDHLSTIAR
                     LKIRNKLKELQKNTVDGINIRCEE"
     gene            complement(21061..21411)
                     /gene="yajC"
                     /locus_tag="PK015_03350"
     CDS             complement(21061..21411)
                     /gene="yajC"
                     /locus_tag="PK015_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOL33539.1"
                     /translation="MIYTLLQAATNSAQAKQSSGASGMSTLIILILLFVVMYFLMIMP
                     QQKKQKELQKMLDSLKENDKVVTTSGIYGRIVSIKPDKNTVVLEIDDNNHVRIEIQKG
                     AIASIVSSAEKANA"
     gene            complement(21425..21910)
                     /locus_tag="PK015_03355"
     CDS             complement(21425..21910)
                     /locus_tag="PK015_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HOL33540.1"
                     /translation="MKSKSLSVETFLASVFGVGFIPYAPGTYGSIVAAAIYLLLPQML
                     FSGKGWLWYTGFLVILIALAVWLSSKAEKKLGPDAPQIVIDEVCGFFLSVLLLPKSFL
                     MAVYALVIFRALDIAKPFPIKISQKLPKGWGIVIDDLIAGVYTNILIHFIKLIAPKFF
                     G"
     gene            complement(21907..23169)
                     /locus_tag="PK015_03360"
     CDS             complement(21907..23169)
                     /locus_tag="PK015_03360"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOL33541.1"
                     /translation="MKKLILILLTSFLIFSLSAELVDRIVAKVGSDIILLSDVYKMMY
                     QMQLAGIPKEQISMDMALREIIEQKVISQKAKELNIKVDDDKIKRYAQNYLQELKSQY
                     PSPQEFQAALAQENLTEAELLEYFIEEIKNNAMTQQLIDLYVSSQVSVTEEEMREFYN
                     TTKDSMAVKPTTWETGMIKYKITASKATEDSIYKQMQDIKKRLDAGADFATLASEVSE
                     CPSKVRGGDLGYFSRGMMLKPIEDAAFSMDIGEISGIIRSENGYHILKLTDKRRDEVR
                     ASHILKLVQPTKSDTLAAYAIMERIRELYESGQSSFSDLALQYSTDPDVTKNKGIIGE
                     LTENEFPEPFKNYILSTPVGKMTPVLEKDGTLYIFVRLKEIPPRVFTYDEVKDKLYTY
                     LYQYKLQSAYNKWIDKLIKETYVEILEK"
     gene            complement(23194..24009)
                     /locus_tag="PK015_03365"
     CDS             complement(23194..24009)
                     /locus_tag="PK015_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33542.1"
                     /translation="MKRFLIAAIMVFILCSSCELKKEKEEFTPLAEVNGEVLTLEAFR
                     SLYTDEQWNKLSETQKRKEIENWVNLTILAQEADKEKISEENATKLRIDYAVKKVKAN
                     ALLARKLANLTISEEEMFNYYRLHQADFTKKLMEYNIQRILCPDENAAKSLLNKIKTG
                     EYDFNLAVLENSLEELKYKQGRMGFVSANSPDSLFWKAAHKLKINEPGIVTVNGKTYI
                     IRYTEQREGTQEATFVDYRNEIYELLLKEKKQQVYNDLVRELKMKNNNIYYYY"
     gene            complement(24013..25254)
                     /locus_tag="PK015_03370"
     CDS             complement(24013..25254)
                     /locus_tag="PK015_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33543.1"
                     /translation="MKTIQLQKMRLLLVATLFLAIILGGCINDPNPTGNNWSNVRPMS
                     FTTPLDIVAGYSFPGSGSIKGTETSLVCGNYNGIESVAFVRFTGLPSVGTFHIPEAYQ
                     DSTYLNLVLTRKFSESRNPVEVKIYKLNQSWAADSTNLIQDSNLSLITPQSYTITDSI
                     SSAGTQIKIPIPVAALEQWTSEADSLGLTLAIKTGENSYIEFKSRESGAGPKLHFLYR
                     TTGENPTEKKYDQYCNKDSYRIDEETAPIITNQWILSNIPPSRIYLYFPMNFSLFQDT
                     EGNPLNEIQLKRTTINHAELILYVKENPYYGTSTQYRLRADRVNDSLDINTAVAVLDE
                     QVASGIITQSYIRGDSLVVNVTPLIQAYTSGDKINYGIVIRSMQELINYGKIELWHFN
                     DSSTPAEKQPKLRVTYTPPFL"
     gene            complement(25513..26844)
                     /gene="dnaA"
                     /locus_tag="PK015_03375"
     CDS             complement(25513..26844)
                     /gene="dnaA"
                     /locus_tag="PK015_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOL33544.1"
                     /translation="MDQDIWQNILDKLEENINPQSFRTWFSETKLVDISDNELVVKVP
                     TQFAANYLNQNYTDTLGEIAYALYKQHYKIKFISPPHYVKNNPNQTAGYSDNRVMVNT
                     KLNERFSFEEFVVGRNNNFAYSAAKAVAEAPGYTYNPLFIYGESGMGKTHLMQAVGNY
                     VVKEGRNCSIYYTTSEEFTNEMIESIRANKMPEFRSKYRKVDLLLVDDVHFLSKKEGT
                     QEEFFHTFNALFDNRKQIVLTSDRPPKDIPDLEKRLVTRFESGLLCDLKNPDFETRVA
                     ILHKKAEPENIVLSDEVFSFIAESITSSVRALEGSLIRILAYASYNNLNPEELDVETV
                     RSILSDMISEQHKEITLDTITQEVCLAYNITMPQIVDKTRRQQISYPRQIAMYLANLL
                     IPQLSLKEIAEYYKRKDHTTVIHAKRMIENRFREDINFRSHIEQLIKNIKS"
     gene            complement(27409..>27612)
                     /locus_tag="PK015_03380"
     CDS             complement(27409..>27612)
                     /locus_tag="PK015_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33545.1"
                     /translation="LDYLEKNDPADEIEVISRLSYEKEWDSIPLELKQKILEIDKIIL
                     DKYASNFNYPLWKRFIQILKSHQ"
BASE COUNT         8646 a         4886 c         4834 g         9146 t
ORIGIN      
        1 ttatcacact ctaaaaatgg attttatagt cagagaataa gttattatat cccagattgt
       61 atgaatatca tcatattttt ttatcaccat taatcctgat atatctataa tatacttgat
      121 ttaatcggga gaagtatctt tcttttagag aaaaaagggt ttcttcttgt caaaatactg
      181 attattatat tagtgtattt tcattaactc tttaaaaaga aggagaaaaa aatggctggt
      241 actttaattg gcatacttgt cgctatcttt attatagttt tgatttccag gggaataata
      301 gttgtccgac aagctgaagt agtcatcgtg gaacgtttag gtaaatatta tcggacttta
      361 gattctggta tacatattat tatccctatt attgataaga ttcgtcctat ccattggcga
      421 tacacccgcg aggattatag aggaaacatg gtagtcaccc aaagattaga aaacagaatt
      481 gacctgagag aaacggtata tgattttcct cgtcaaaatg ttattaccaa ggataatgtt
      541 tctatcacta tcaatgctct tctttatttt caggtaacag acccttataa agctgtttat
      601 gaaatcagta atttacctga agccattgaa aagctcactc aaacctcatt gcgtaatata
      661 attggtgaat taactctgga ctacactctc acctcaagag atatcataaa tgccaagtta
      721 agggacattc tggatgaagc aacggataaa tggggagtaa aggtcaatag ggtagagctg
      781 caagagattc tacctccaga agaaatacgc actgctatgg aaaaagaaat gagagcagaa
      841 cgggataaaa gagctaaaat cctacaagct gatggtgaac gagaatatca agtgcgagtg
      901 gctgaaggtg aaaaacttgc taaaattgcc cgagcagatg gtgaagcgca ggcaaaattg
      961 ctggtggctg atgcagaacg taaatctatt gaattgattg cagaagcagt gaaaaatacc
     1021 ggaactgacc ccgctcaata tcagattgct cttaaatata tcactgcttt ccaggaaatc
     1081 gttaaacagg gtgataagac tgtagtggtc ccttatgaag ccacagctat gcttggttcc
     1141 ctaaaaacaa tggaggaact ttttcataaa taaaatacaa aggagataga agtgtctgaa
     1201 atcgtctaca ttaaaggaag agaaatactg gattcaagag gcaatccaac tgtggaagca
     1261 gatgtgatgc tggatagtgg tgtttttaca cgtgctgctg ttccaagtgg agcatccact
     1321 ggagaaagag aagctataga attgcgagat ggcgataaaa aaagatatga cgggaaaggt
     1381 gtcttaaaag ccgtggaaaa catcaattcc atcattgctc ctgctctgct aaatattgaa
     1441 gcaactcacc aagaagaaat agattcggtg atgctaaatc tggatgggac gcccaataaa
     1501 agcaaacttg gagctaatgc tattctggct gtttctatgg ctgttgccag agcaagtgct
     1561 attgaactgg accttccact ttataggtat ttaggtggag tgggtgcaaa aacactgcct
     1621 gtcccgatga gcaatatctt aaatggcgga gttcatgctg ataacaatat tgatattcag
     1681 gaatttatga ttatgccatt gggcgctaat tccttccagc aagccttaca gatgaatgca
     1741 gaaatctttc acgctttaaa gggtatcctc aaaaaacagg gtctattcac cggtgtaggt
     1801 gacgaaggag gttttgctcc aaaccttaaa gacaatgaag aagctcttaa agttattatg
     1861 gaagctatcg ttgccgcagg ttatcaacca ggaaaagatg tctttattgc tcttgatgtt
     1921 gcagcttcca gtttctggaa agatggtaaa tatctgtttg aaggaaagac attctcctct
     1981 tcagatatga ttgactatta catcaatctt gtccagaaat atcctattat cagcctggaa
     2041 gatggattag cagaaaacga ctgggagaac tggattattc ttacccaaaa actcggaaat
     2101 aaaattcaac tcgtaggtga cgaccttttt gtgaccaatc ctgctataat taagcgtggt
     2161 attgccgaaa atgctgctaa cagtgtcctt atcaaactaa atcaaattgg aacagttagt
     2221 gagaccattg aggcaattca aactgcacat aaagctggct ggaccagtgt ggtaagtcat
     2281 cgtagcggtg agacttgcga tacttttatc gctgacctcg cggtggctat gaacacggga
     2341 atgattaaaa ctggttctct ttccagaagt gaacgtatag aaaaatataa tcaactactt
     2401 cgcattgagg aagaactggg ttccgcagca atttatcctg gtagagcagt gattaaacag
     2461 ctaagttaat ccactgcata tcattatatt aaacaattat aaaggcaggc taatcctgcc
     2521 tttttctttt ttctaagctt taatctaaaa cttttataaa atcttgcatc aagtcttaat
     2581 caagcgttaa tcaagtcttt atttattaac gcttgattaa gatgtgatta acacttgatg
     2641 ccaggaataa gctaagagaa tcacacttat aaatttaaaa tcattcaaaa actaattaat
     2701 cttccagatc ctatttttgt attgatttat ttgaaagaat aataactttt ccagcaaaca
     2761 aaataatcag cttaactgat tcataaatat aataattagt atgctataag atagttatag
     2821 gagaaaaaat ttaggggaaa aataaaaaat aaacttgaca aataaatagc ctaaatttaa
     2881 tagaactatc ctacatagaa tgccctccga taccgtgaag acaccgcccg gtgctcttca
     2941 cggttttttc atttattctc taagagttag aactcctgtt aacaatgttt cagtaaccct
     3001 attttattaa agatatctta cgggttatag tgttttgagg agtttttaca ttcaatagat
     3061 agattccatt tgcaactggt ttaccctgct tatcttttcc atcccagatg aggttatgat
     3121 tcccttttga tacaacttcc cagccaatta tttgttgacc tttgagattg tagattgaca
     3181 ccgtggcttt atcttcagat ttcagggtta aattaatatt agttatatcg gtaactggat
     3241 tcgggtaaat gctaataata ccgatggatt ggtattcatc actaatagga gtggcaggaa
     3301 tttctattat agctggcact gattctcctg ctacatactg agcggtaact ttcactatat
     3361 gagccacagt aaaatcccaa ttaaagaaag agtagaatgt aaaggtcgtt gaatctacga
     3421 actcattatc cagatagatg ttgtaatgtt ccagaggaga ggtacttcct tgaggctcct
     3481 cccagctaat agtaacaggt tcatccgtat tagctaataa atttagtgga ggatttaaaa
     3541 ctgcaggtga atattttaga ttacgataaa atttgaaagt cccatcataa tcacctaaaa
     3601 cgagatcaag gtctccatca tggtcaagat ctgccagagc aggagccgcg ttttgattcg
     3661 tttctatacc agcaaacaat tctgtattat taacccagtt atgattttga tatagataac
     3721 aatgtagtga accgaacaaa tcacctgcca ataggtccaa tcttccattt tcatccaggt
     3781 caccaaaagt gggagaacag tctgagccaa catctatatt gccaaaaaat ccgctaacta
     3841 aagtccattg aggtaattct ggcgaaccat tattttgata ataataaata ttccctgctt
     3901 cattgccaac tgccaaatcc aggtctccat ccgaatccat atcaattaag cgaggtacac
     3961 tccagccacc aacagaaata gggggaagga cattattcac ctgaataaaa ccggttccgg
     4021 tatttttatg gaaataaaga tttccactca aatcacctac tatggcatcc ggcagtccat
     4081 cagcatccac atctccaaga gttatagcag aataaatgga atggtctata ctggaaaaat
     4141 aagagctatc ttcttgccac gctggagcat agggagttcc tatattttta taaaatttta
     4201 tatctcccag ctgtgttcca caaatcaaat ctaaatctcc atcaccatca aaatcaaaca
     4261 ggacaggaga actcgctcca ccagcatcta taacaccacc aaaaagatta gtattctctt
     4321 gccattgagg agaagatgga gttccattat tcacataata ctttaagggt ccatctgcag
     4381 ttccgaaaat caggtcaaaa tccccatctc catctaaatc caccaggtct ggagaattcc
     4441 aataagtaga atttcctaag ccactaaaga gggcagaatt attttcccat aagggagatg
     4501 agatagttcc attattttga taatagacaa atccatgcgc atccctgcca caaagaatgt
     4561 cctgatcacc atcattatcc aaatcacaaa aaacaggata agcataaaga ccaacatctc
     4621 ctattagctg cattgattct tccagaaaaa taccattgga agcagtacca gtattaaaat
     4681 agactcgcac agcaccatct tcacttaaac cgaccactaa atccaaatcg ccatcaccat
     4741 ctacatcagc aacatcgggc acgggattat gtcctacttc cagagcagaa aaataaccca
     4801 gctgctctgt ataaacaggt gattgaattg ttcccgtatt gagaaataga tgaagaccgg
     4861 tataaccacc ggtaattaaa tctaacttcc catctgcatt catatctgcg caaactgcta
     4921 attccgcatc aataaaggat atattggcta aaagattatc gccaataaca aaatgaggag
     4981 aagttgctgt gccaatatta cagatatata aaggtgcact attagtgttt cccagaaaga
     5041 aatccatatc tccatcagca tcaagatcta cgaaacgagg ttgagaaaag ggtaaactgg
     5101 gaattccact gggattaaaa atcgcattat cctgttgcca gggctgagca aaaatcatta
     5161 caggcaacaa tagtagtaat agtaggtatt tattcatttt tatgactcct tgtttcttat
     5221 tttaatctta taatatattg atgtattatc agttaatctt tattgtaagt tattcaaaag
     5281 tttgaaactt aaaattctaa acttgaaccg ggaactcata gatttgaaac catggcaaag
     5341 atattataac gctcctgacg cgaaatgcta tgtaaagtta tatgattttc ttggtgataa
     5401 gcaagtagag aaagtgccag ctgagaagcc tttttgcccg aatcaagcaa agttctccat
     5461 tcagagttaa actctttctt attccagtaa agatttagta tcttaccgag ggcatttaaa
     5521 gtagaaagct ctgcaaaaga aagggtatca gttcttagta cctgccataa aggagtatcc
     5581 agccagagat aatgtttttg ctttgccaaa tcatacattg gtgtatcagg caatattttt
     5641 aagctaccta actgtatttc atcaggaaag atagatgcca attcatcaag agaatgcaac
     5701 accgatgcca ttgactctcc tggtaaacct gctaacaggt ctgtatgaag ctgaatctga
     5761 gtattctcgc ataagaaagc taaggatttt tttgctttct cccaattaga cattctacca
     5821 caagctttcg caatatcagg attgaccgtc tgaactcctg cttccagacg aatcctatga
     5881 gcaggtgctt tagctaatag tgtgaggtct tcttctttta aaagttcggg aaaaatttca
     5941 aaatgaaact cacaatctgc ttgcatttga atgacataat cccagataag atgagctaaa
     6001 tcaggatttg agttaaagca acggtctaca aatttcacgg ttcggggctt taaagttaaa
     6061 agctgctcca tttcattgta taatcttgtt ctatcttctg agctttctgg attaaaacgt
     6121 agttcatttc ttttatctct tgcggaaaga cagtaaatac aatggaaagg acaaccccgc
     6181 gagcattcat aatagaccaa ttttccttcc agtgcagccc tatcttcttc tttataaaga
     6241 aagggaagtg tatttaacga tggagctgga acctcatata ttcctccttc cagagaaaaa
     6301 ttattctccg ctaaataacg aaaagctcct tctcctgctc ctttaactat aaaatcagaa
     6361 tcgttcaatt tataatcttc gcaaagagca gaaggacctc caatgacgat tttagcttct
     6421 ggaagaagtt tcttaacttc agggattaga agctttagat acaattgatt ccagatataa
     6481 gcagaaaaac ataagatatc agctttagaa ctaactatgg tgctcagtac atcaaaaagt
     6541 ggttcatcag caataaattc atatatttca atatgataag gaagaccttc taaggcattg
     6601 cgtagatagt ataaagcagg attgctttga taccaagcac tattaacggc tataaaacag
     6661 atttttttgt ttccttgatt gctgagcata gattattttc cttatttcct ttactgtcta
     6721 aaagaatctg tcctttctgg caaggaaaat ataactttga taagaacata atctctaatg
     6781 agaataataa acataatttc cgtttataaa atataactta aaagaagatt gaaaataaaa
     6841 gaacttgacc taaagatata atacattagt taagaataaa ttttgactaa agtgaggcta
     6901 atttatgtta ttagcagatt ttctggagcc cagtactata gtttttgaac aaagaattct
     6961 aactcgggaa gaggtctata aagatattat aactcgtatc tgtcagttaa gatcagatat
     7021 tgtacctaat tgtggagaaa cacttcttga gttaatactg gaaagagaga aggaagctcc
     7081 gatggtttat ccttcaggta ttgctatacc tcatattcgt ctggatggat tagcagacac
     7141 acttatcgga atgacctttt tacagaatcc tctgaattac gatggcataa aggttgactg
     7201 gattgtaatg atttttacgg ataagacttc ttcaaagata tatttaaata ttgtggcagc
     7261 tttacttaaa atatccgagg atgaagagat gatgactcaa ttacgctcct ttaatgatgg
     7321 ttatgcagta gttaactatt tacgacaatg caggataaaa gtaggacagg aactttgtgt
     7381 ttccgatttg atggtaaaaa atcctatctg tgtcactcct gatactcttt taagtaaagt
     7441 agatagtctt atgaatgtac ataatatttc ctgcattcct gttgtagata atgaaggtag
     7501 atatctggga gagattaata ttctgaatat tctaaaagtt ggaatgccag actatctaat
     7561 gatgatagat aatgtagctt ttttgaggtc ttatgagcca ctggaaagtt tatttgaaaa
     7621 agaggaaata gttaaagcag gagagataat gcaaaaaaat acacgagtgc tctcttctga
     7681 tgcctccatt gtagagactg tgtatcatat gcttaaagat aacaaaaggt atttttgcgt
     7741 ggtagataac ggacgtttag ttggtattat tacagctatg gatattttca ggaaggttat
     7801 aaaagcatga gtgcaacagt cagtatgtta atagcagtag gtatctttat cggtacctat
     7861 atattaataa tcacagaagt aataaacaag atgcttgctt cattgatagg agcattcttg
     7921 cttattctaa caggtattct aagccaggat attgctctta gagctataga ttggaatgta
     7981 attttctttt taatcggaat gatgctggtt attgcggtat tacggagaac aggaattttt
     8041 atgtatattg ctatcaaaac cgctaaactt gcgcgtggaa atccgttact gattatgatg
     8101 ttgatgtttg tggtaacagc tttctcctct gcttttctgg gaagcgttac ctctataatg
     8161 attttaatac cgataatttt attgatttgc aatgaactaa aaataacccc agttccttat
     8221 ataatcacga tggtggttgc ttccaatatg ggtggagctg ctactatgat tggtgaccct
     8281 ccaaatataa ttattggaag tgccacggat tatgatttcc ttgatttcat ttttaatctg
     8341 actccagcaa tcattctggc tacgattgcc tgtcttggtt taatgtgggt tatgtatcat
     8401 ggcaaattga aagtgagtag agaaaataga gccaggatga tgagttataa agaagaggga
     8461 ttgattaaag ataaaagaat gctcaaaata tctttaataa ccgttgccgt tatgcttgtt
     8521 cttcttgcct tagaaagtac tttgaaaata ggaacagcat ctatctctat gtctgctgga
     8581 ttattcttaa tcctggtagg ccaacgaaat gatattgagc atataatggt tcatgaagta
     8641 gactgggtta cactcttttt ctttgtggga ttatttatca tagttgaagg cttagttcaa
     8701 accggattta ttagtttatt agcacataaa gttctggaac taacccataa tcaagcacgt
     8761 ccaacttcta tggctatact ctggctttca ggtattcttt ctgcctttat tgacaatgtt
     8821 ccctttgtgg caacaatgat tcccttaatt aagcaaattg gcactgtaat aagtcaacct
     8881 aatcttatgg atcctatttg gtggtcaatg tctttgggaa cttgtttagg tggaaatggg
     8941 acacttattg gtgcctcttc caatgtggta gcaattggaa ttactaaaaa gaacgggatt
     9001 aatatctcat ttatggaatt taccaaagta agtgtggcat atactatcct ttctttggtt
     9061 atcagttcag tctatatttt aattcgttat ttctaagatt gataaagctt gaattactcg
     9121 gtgatatcta ttggacggat acactcttca gaattgtttt gaggattgtt taccactcta
     9181 ctaactggat acatagaaat taaatcatct ggtggagctt tcagtagggg aataaactta
     9241 tttatgtctt taattgtatg ctccagccag gttaaacggt catctgcaaa tagcaaaacc
     9301 ggcatcctat tatgaatagg aagcattacc tgattggcat ccgtagttat tatggctaaa
     9361 gaggggacat aacttccctc aggactttgc cagacatcgt agattcctgc catataaata
     9421 aagtcatcct gggctgaatg aataaaaaat ggttgtttat ctgtcttacg ccattcataa
     9481 aaaccattag ctggaatcag acatctgcga tattgtaagc ccctcttgaa tgaaggtttt
     9541 gaaattaagg aatcggaacg gatatttatc aaatcaaact taggaagttc ctgactccag
     9601 gaaggaatta aaccccaccg aaaagtgcca atgtatcttc cactatcctg gctcactgct
     9661 gctcccacag gttgagttgg tgctacatta aaattaatcg ttaactggtc actttccctg
     9721 tttaatttta gttctttttc caacctttgt aactggtcat attttataac ttgggcaaat
     9781 ctaccacaca ttgttatctc cttcttattg acaatatcta tcagtttcta tatcctgcaa
     9841 catattttta actctatttt atgataatta aagcggatta aattttatga ataattttga
     9901 tattgatact gtgatggctg aactggaaaa gcactttaca cgagttagaa ctcctgtagt
     9961 tgaccagata cagattcaga ccaaagatcc atttaaggtt ctggttacta ctatccttag
    10021 tgcaagaaca aaagatgaga ccacctctca agcagcaaag aagctatttg aaaaggtaaa
    10081 gaatatagat gacctggaaa agattagtgc tgaggaactt gattctatca ttcatcaagt
    10141 aggatttcat aatgccaaaa ctaagtattt gaaacagttg ccgtcggtgc taaaagagaa
    10201 atttggagga aaaattcctt ccgaaataga tgaacttcta cagttacctg gagtaggaag
    10261 aaagactgct aatcttgtga gagccatagc cttctcactt ccggctattt gtgtggatgt
    10321 gcatgttcat agaatatgca atcgctgggg atatgttcaa acaaagaccc cttatgaaac
    10381 agagatggaa ttgagaaaga ttctaccgga acgatactgg ttaactttca attcctatgt
    10441 agtggctttt ggtcagaatc tttgcactcc aagaaaaccc aaatgcaatc aatgccctat
    10501 atataatcaa tgtgccagaa ttggagttaa agaatgatgt ccttactgac tgagctcttt
    10561 ggcttcttgc agccatttag agtagtatct tgcttttcgt ttattaccta ttttatcata
    10621 gaatttttcc agtgctttaa ctatataaag tgtggcatta tgatatttcc caaagtattt
    10681 acgatcaata gttaagcact tatgtaacaa tgcttcagct tcattccatc tttgttggtc
    10741 cataaaaata gatgccagat ttttgaagta aataactatt tgaggatgat tttcaccata
    10801 tatctttttg cctatttcaa ccgctctttg tagaagtttt tctgcctctt ctatctttcc
    10861 agactccgca aaactcatcc ctaagttatt acaaacggtg gcagtaaaga aatgataggg
    10921 gtcataattt cggttcatta tatcataggc ttgttgatac atttccgtgg ctttatcatt
    10981 aagattttgc agtgaatata aatgtcccag attattaatt ctatcagcca cattggaaga
    11041 atcaggctca tgtttcttct ctaattccag tgattgtaga agcaatcttt cactttcttc
    11101 atagcgacca acatctttat agaaccttcc caagttatta atataaggac ttaacttaag
    11161 actatcttct ccatatgtgg ctaaagcaat attcatcaca gtatcataaa gtttttctgc
    11221 ctcttccatc ctttgcatat aatgatagaa gagagcgaga tgaaatacat tttccccata
    11281 attcatatta tctgtaccaa aagatttttc agctaattca accgctttta gaaagcactt
    11341 ttcagccaat tcaaaataac tattctccca gtaataatct cctgctttat cataataatc
    11401 ggaggctttg atgatttctc cagctttttc atagtgtttt gctatttctt cggagtattc
    11461 acttatattt tgagcaaata cttcttccat agattgagcc gcttttagat gtaaactttt
    11521 tagcttatca ctaaacattc tattataagc agcttcctgc aaaaggatat gagtgaagat
    11581 ataaaaatct ctctctaaac ttttccagat acggttttgt gtgcctatta aaaggtcttg
    11641 ccggatatct ctttttagca tctgacttaa tactttcaga ttaaatttca tacccagaac
    11701 actggcatta tagatacatt cacgcactga aaaaggtaaa cggtctatgc gaaaaccaat
    11761 tatgtcattg acatcaaaag aattatcttt ccccaggata tttagatagt ccgcactatt
    11821 ctcaagcaca ccactttcta ataaataaga tgccatctgt tcaataataa agggatttcc
    11881 tgtatccttc tgcagaattt cctgtttcaa atcagctggc acatcatcca gattcatcat
    11941 tgatttatag agctgccagg aataattttt acttaaagga gcaaggtcaa aacgataaaa
    12001 gctaaaatta tccatattca aaattggtag cctatcattt tcaatataac gagtggttgc
    12061 taaaatacct gtatctttta tatctgattt tcccaaaata tggaagaaat tacgagattg
    12121 ttcatccatc caatgtaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa
    12241 tggtcgggaa ttggtaatgc tacacataat atgaaagatt cgtatgttct tatcttggca
    12301 ggagttaatc acttctgtcg ccaatctggt ttttccaatt ccagcttcac ctgcaatata
    12361 aataaagtaa ttatgatgct ctgctattga ctttgagata atcctctgta tactttttaa
    12421 ttcattttgt cgtcctacaa ataccgtcct tagctcagga acagatggtt gcaattcctt
    12481 taatttataa cagggaatcc tttttttcaa tccctttaaa gaaagattac caacaaattc
    12541 aaagtcaaaa ttcctttcta attccttttt taatgttata tcggttaaaa tctcaccctt
    12601 tttacttttc cccgatagtc ttgcagccaa atttacggga tgacctaagg cggtatattc
    12661 acggatatgt tctcctccaa caaagccagt aaaacaaggt cccattgata ttgcctgact
    12721 tataaaggga tacttatgaa ctctttccaa aataagttga aagacctgac tgagcttttt
    12781 accttcattt cggggcattc catagtagga caaaatgcaa agagaattat ttgtaaccga
    12841 taatttatct acaaaggatc cagaatattg atagaagatt tccagattgg ctattatact
    12901 gcagatatcc aaatctgctg taaaaggtat ccgggtgtaa ttgctgataa tggttctgat
    12961 ttcattctct ggatggatat tatgcaaacg ataatgaagg aatttgtatt cagtctcttt
    13021 ctgataagga taattaatta tattttcttt taccttttct ggattgaagt cccgacttat
    13081 ttttccctta tcatccacta atcttgaaga gattagtttc tgtaaagccg aggtggaata
    13141 tctacaagtg gtttgacctt tatttaaact ttgaacctct tctattgcat cgccaaagaa
    13201 gacatactcc atctggatat cattggagaa tatcatccat tgaaccgtac cataaccgat
    13261 tctaattctc atttttaccg gagatttatt taaaagtctt tgtttttgat attggtttct
    13321 tagaaaggat tgaatatagt gtaaagcagc cataatattt tctggctgag caccaggaaa
    13381 aatagcataa aaagcatctc cagcgaaatt agataagaag cccccatatt tttctatgat
    13441 atttaatggt tcactaaaga tattagttat taagtggcta acttcttcag caccttcttt
    13501 tccttttttt tgtaattcat catttatggt agtaaagcct atgatatccc cgagcaggac
    13561 aaaagcttgc attttgcctt gaacattgtc tttttcatat tgagaaagga tgaagggtgg
    13621 gatataacga atcataattc tacctgaaag aatatgatga ataaccgtta aataatagct
    13681 acataacagg gaaagtcctt tactacaaga cgataaatag cctcagcgtc caattccttc
    13741 caacaaaatg tttcacatga aaccacggat tgagctaata aggctgatat tccacctaca
    13801 caaataaaat atactgcatc gtatttagat atctcttctg tgacttcttt agacctttct
    13861 cccttaccta tcattactct taaaccattc tccagtaata aaggagtata tttatccatt
    13921 ctggctgagg ttgttggtcc tatggcacca catacttttc caggtggaat aggagcaggt
    13981 ccgcaataaa agagtgcact atttgacaaa tcgaaaggta agggtttccc ttcttttatc
    14041 aattgagtta atctaatatg agcttgatct cttgctgtat ataattctcc tgaaagcaaa
    14101 attttatctc taactttgag ttcttttttc tgttgtggtt gcaaaggaag attaattttt
    14161 attactctca tatttattct cttatcttat tattattcag acagatatgg agatacaacg
    14221 atgagcatga caatcaatat taactgctat aggtaaagaa gcaatatggc agggttgaga
    14281 taatatattt acttttagag cagtggtttt acctccaaat ccctgtacac ctttgccttt
    14341 ttgattaatc ttatttagga ttcttttttc catctggagg tattcaggtt ttttctcaga
    14401 atcagggaga ggaatcatta acgctctttt agcaagaata gcacattgtt caaaatctcc
    14461 tccaattcca atacctacaa aaacaggagg acagggtttt ccacccgcag aaacaacagt
    14521 gtctataata aaatcttcaa tttctgttaa ggttgctgta ggattaaaca ttttaagggt
    14581 gctgcaattt tcagctccac caccttttaa ggatatatgt agttccaatt tatttcctaa
    14641 tatttcggat aaatgtaaga tgacgggttg cgaatagtat tttgattttt tttgagggtt
    14701 agagaaatac cttaatggtt cttcagcgat agaatctcta aggtagtatt ttccccaagc
    14761 atcattaact gcttctgata ctatagaata aagtgttcca ttttcaattt tagtttctgt
    14821 tccaatatcg gcaaaaacaa ttactatgcc agtatcctga catataggaa gttgttcttt
    14881 agcagcaagt tcttcattta atagcatagc ttgaagcata tcttttgcca catcatcttc
    14941 ttcaatggct aaggcttggt ttagcaaaga tttagtttct ggagtagatt gacaatatat
    15001 ctgcccgatt gcttcaatta tagctttata tatctgtttt ttgggaatag ttcttacaga
    15061 ctccattcat ttatcctttt cttcgcatta aaagatattt caacagcagg tcttctatag
    15121 gagtagcagt gctaaccgga ttatattcaa tttgatattg atgacatcca gccttaagtt
    15181 tctggatatg ttcttgataa tttagcagat aatcgggtct tatttgccaa ggattgacgg
    15241 taattttttc ttgcgtttcg ctatctatga attcagtttc accgtgaaat ttaaaattat
    15301 cttcttgtat atcagtaata tggaacacca atacttcatg atgatgagat ttaaagtgct
    15361 ttagtccatc cattatattt tcaggttcat ccaaaaggtc agaaatcagt attattaagc
    15421 ttcttttacg gatagtatca gcaattttat gtaagttttc cagaagatgc gtggaatcct
    15481 taggttccaa ttcagccagt atcctaaaaa tttgagggag atatcctcta tatgctttgg
    15541 gaggaagtag cttagtgatt tcattattga aggtaattaa tcctgcggca tctttttgac
    15601 ctatcataag ccaggaaaga gaagcagcaa gtcggcaagc atagtcaatt ttagtgtttt
    15661 caccagagcc atagaacatt gaacgactgt ggtctaaaag gatatagcag cgtagattgg
    15721 tctcttcttc aaagcgtttc acatagtatc tatcggtttt agctacaatt ttccagtcaa
    15781 tatttttaat agggtctcca atattgtatt ctctatggtc cgagaattca acactgaaac
    15841 cgtgataggg ggatttatgc agcccaacca aaaaaccttc aacggtagtt ttagcgagta
    15901 gctgaaaccg ttttagtttg gctgcagtct catcagtaag tagctgttgg ttcattcctt
    15961 aggtttactc ttatagagca gccccagtgg aattagaacg ccatatgcga tagtaagtat
    16021 tacaggagag ataacgattt catttcctgc cattataaag tagcctacaa ttagcaaaat
    16081 gacagcgatt cctaagatta tatagttaat aggtcctaac tgtaatttat tattatttgc
    16141 cattagcatt ttctccagat attatgatcc tgcaacacaa ttttcatagc tttcaaaggt
    16201 attgcttact ttttcatagg ttaattgaca gagttcgctt ctatcacgat tagggtcaaa
    16261 ctctattggt tctaagaaat tgatttctac ctccatttta tgtgccagca gattccagta
    16321 gtattttaaa taattaatgc tcttagacca gaaaacgacg aaacgatttt taccattaat
    16381 tggtttccca tccagtttca gataacgaat acagacagga acgacagtgc aagcagcatc
    16441 tatggcagtc tgaaagaggg agggacggaa ttcattgact tttgttccat cggtgccagg
    16501 tttttcagga aatagcacaa ctttaaaacc tttttttaac acatcagtga agcgtttgat
    16561 ttcttgaggt agatttaatt tatttttccg gtcagtataa agacatccac caagacggat
    16621 aatattacca ataataggag tttcactcat ttcggtggag gtgataaata cataattgtc
    16681 aactgcacca agcaaaaata tatctaataa agatgaatga ttagctacca gcagatagtt
    16741 gttatcttta agttgttgaa gcacttcctg attctttact ctaagtttga catggaaagc
    16801 acgaagaaat tttcgggaag aattgctcat atttttaatc tgtaaccagc ggcgtttaat
    16861 tggattttta gcaaaaagac ggataaaata ggaacgacag atgaaagacc agagtagtat
    16921 catctgccag atagtatgaa ataatttaac tatccacatc attttatcct cttttttatt
    16981 atattcttct tttctataga tatcgtaatc tgtcaagttt ttttctgctg gtttctacca
    17041 taacctcaag aaaatatctt ttatgcttta accttatata acttttttaa gcttttataa
    17101 tttaattcat tccgagaatt atgaaaaggt ataattattg ttttttaccg ttataacaaa
    17161 ttataaaaac atctctgcag tttatttctt ctttcttagc agtttatcca ttactgatgt
    17221 aaagattatg tccaaatcgg acataagctg gacatgagtt ggacatgagc tggacataag
    17281 caacccatca agaggaaaaa ataattgggt ggatttatat ttcaaaagct attggttcta
    17341 aagaaggtag gatttgcttt acagttggta ttatggttta gggaaggata ttaatttaaa
    17401 aagaggaaaa ctcacgggaa aataataagc cggattctgt acctccttct atcaggaggt
    17461 tgatgtccat tcctctacat ccattctcac gaatggactt tagctgccta cccggaagcc
    17521 ataatcgtgc gggcaacact tcttggcttc cctatttggc attgcaccgg ataaggcttt
    17581 cctggcaaat cctgtcacca ggatttccgg tggtctctta caccaccatt tcacccttac
    17641 ctgaaataga tatttataaa ataactattt caggcggttt atttctgtgg agctcgctct
    17701 gcattactgc agcttcctgt taggaagtat cctgcccttt ggtgtccgga ctttcctccc
    17761 ctacttttca gtagcagtgg acatctcatt ttcccgtgag ctttaagata taatatataa
    17821 aattgttatt ctgacaagaa aaatattttt cttgcttgct caaatttaat tgagtttctt
    17881 actactgagt tatgaactaa ttcagtttaa gtaaatttta gaaagaagct atccagttat
    17941 aggattttga ttatagattt aactctatct atgggtatag cgaatccaag tccgacatta
    18001 tctcctgttc cagaaacgat aaaggtatta atgccaataa cttcaccttt aacatttacc
    18061 agtggaccac cgctattacc aggattgata gcagcatcag tctgaatcat atttatgtat
    18121 ctatgaccat cttgaggctg gaaacttcta tttagagcag aaataacacc aactgaaacg
    18181 gatggtttgg ggtcactcat cataaatcca taaggatttc ccaaagcaat agcccattca
    18241 ccaataagta attctttggc ggttccgagt ttggcataag gaaaattatt gccatctatt
    18301 ttcaatttag ccacatcgta tgtgttatct acaccaacta cattagcaat aaattctcgc
    18361 ttatctggaa gcacaacttt gatttcggat gcaccttcta ttacatgagc attggtaata
    18421 atataaccat ttgcatcata gataacacca cttccgatac taaccacctg tctttgaatg
    18481 ggaatgtctc caaagaaatc aaagatgcta aaaccaaaac ctggtctcac taactgcgtt
    18541 ttggtcacat taacactaac tacagctggt tcaactgctt gaactgccga ggtaatttca
    18601 ttatggcgtt gattatgtga agggtctaca atagtagtag tagaagcagg cgcattgatt
    18661 tctttctgat ttatgagtac caaagtgata ccagcattaa gaataagtac agccaaggct
    18721 ataataataa tattagatgt tttcataagg catcctattt aaactgttta atggcaaatt
    18781 caataaaaga gatatttact ggattttttt cattggcagt aatagattgt tcatttaaga
    18841 ctgcgtaaac aggataattc cagttatctc tcaggctatt aaccagttct ttagcagtag
    18901 caacagccac aataacttct ggattttttt cttctatcat tttccgttct aaaaaacctc
    18961 cggtagcaac tgctgcatca acattctgtt ctgttataag tttcttaatt ttagccatat
    19021 cacatccacc gcattcttca catttaataa tatcattggt gatgcgaatg gtgcaatctt
    19081 ctttttgaat acaatgagga agaagaacaa gaatatttgt gttagatata tctttctggt
    19141 cggttaggac aatttcatta ttgaagttta agaaagattc aatcagattc tttttagaag
    19201 agaaagtgat atgggcaacc cctttagcca ggggataata aatgtaaaaa agcatccatt
    19261 tactaagctt ttgaagccag cgcggtttta atctggtatg ggtagagaaa agggtaatca
    19321 tccagcctaa cacgataaaa atgagtatag ctaatagtat aataaataga gttctaaccc
    19381 aattagccag caaaggttga gcaaagacat tgaataatat aatcagaaag acagcactaa
    19441 taattaaaag actgatgctt acaaaagcgg gaaactttat aactgagagg taaaggtctt
    19501 ggtctttcat aattcctccc tgtaagaatt ttcaataaag attttatcaa aagggacaaa
    19561 actttccccc gctgtaagtc ttgctcccaa ttgaaaagcc caggcagaca taactttttt
    19621 accttcagcc tgaattctgg tgattaaaag ttgtccatcc tggcaattaa ctaatatacc
    19681 tttattttta ataaatcctg caatagttcc aggcttatca ttttttgatg agtcataaat
    19741 ctcagcttga aggattttaa ggttagtatt atgtatagat gttcttgcac ctggtgaagg
    19801 agaaaaggca cgtatctggt taaagatatt ttttatttta ttatcccagt ttatccaaaa
    19861 atctttttta ctaaatttat gggtataaga agccagattt tcgtcttgag gtcgttctgt
    19921 tagactatta tctttaatag caggaataat ttcaatcaat agctcggcag agatattagc
    19981 cagtttttct tttaaggaac caaagttatc ttctggtagg ataaccaatt ctctttgggc
    20041 taagatagga cctgcgtcaa gacgagaagt taacctgaaa atagtggttc ctgttttatg
    20101 gtctccatta agaagggcag tttgaatagg agtaggacca cgatatttag gaagtaaaga
    20161 tggatgaaga ttaatagctc caaaagtagg aatctgtcta agttcttttt ttattaaaga
    20221 ggcataggaa gcagtgacca gaagttcagg atataacttg ctaattttat ccacactttc
    20281 aggagaattg atgttttctg gttgatacaa ttcccagcct tgttcccgta caaaacgagc
    20341 tataggacag ggtttaattc tttggtttcg tcctgcgggt ctatctggtt ggctgataat
    20401 tagtagtaat tgatgttttt ctttgagttt ttttaaagca ggaatgccaa attcagagct
    20461 accagcaaag acaattttca ttggatatta gcctcttcta ttcttcacaa cggatattaa
    20521 tcccatccac cgtatttttc tgcaattctt tcaatttatt tcttattttc aacctggcaa
    20581 tagtacttag atggtcaaca aacaggattc cttccagatg gtcatattca tgctgaacga
    20641 taacggctgc atatccagaa agattaattc tgtgtcgttc attttgtaaa tctgtataag
    20701 agacggttat atttgaaggt ctaaccactt tagcatagat atcaggaata ctgatgcacc
    20761 cttcttcggt ttcagtttca ccactacttt cttcaataac aggattaatc ataaccatcg
    20821 gagagtgttt tttagggtta tcctttattt gaggatcaat cacgataatt cttttgctaa
    20881 cgccaacttg aggtgcagaa agtccaactc catcccattg atacatagta gcgataaggt
    20941 cctcagcaaa ttgcaataag ttttcatcaa ttttttgcac ttcctcggac ttaagtctga
    21001 ggaccttatc accataaata tggattggta ataatttagg tttacgcgac attagattta
    21061 ttaggcatta gctttttcag cagagctaac tatactggca attgcacctt tctggatttc
    21121 aattctaaca tggttgttat catctatttc caagacaacg gtattcttgt ctggtttgat
    21181 agagacgata cgaccataaa ttccggaagt ggtaacaact ttgtcatttt ctttcaagct
    21241 atccagcatt ttttgcagct ctttttgctt tttttgttgt ggcataatca tcagaaaata
    21301 catcactaca aatagcagaa ttagaataat tagagtgctc attccgcttg ctccggaact
    21361 ctgttttgct tgtgcgctat tagtagcagc ttgaagtaga gtatatatca tttttttctc
    21421 ctttttagcc aaagaatttt ggcgctataa gttttatgaa atgaatcagg atattagtat
    21481 agactcctgc aatgaggtca tctataacaa taccccaacc cttgggtagt ttttgagaaa
    21541 tctttatagg aaatggcttg gcaatatcca aagccctaaa aataacaagg gcatatacag
    21601 ccattagaaa cgatttcggt aatagtaata cacttagaaa aaatccgcat acctcgtcaa
    21661 tcactatttg aggtgcatca ggtccaagct ttttttctgc tttagaactt aaccacacag
    21721 ctaaagcaat caagatgaca agaaatccag tataccagag ccatccttta ccggaaaata
    21781 gcatttgtgg aagcaagaga tagatagctg ctgctacaat agaaccatag gttccgggag
    21841 cataaggaat aaatccaact ccgaaaactg aagccaggaa ggtctctaca gatagagatt
    21901 tagatttcat ttttcaagta tttcaacata agtttctttt attaatttat ctatccattt
    21961 gttataagca gattgtaact tatactggta aagataagtg taaagtttat ctttaacttc
    22021 atcataagtg aaaacacgag gaggaatttc tttaagccgg acaaaaatat acagggttcc
    22081 gtctttttcc aggacagggg tcatttttcc cacaggagta gaaagaatgt agtttttaaa
    22141 gggttcagga aattcatttt ctgtaagctc tcctataatc cctttgtttt tggtaacatc
    22201 agggtcagta gaatactgta aagcgaggtc tgagaaagaa ctttgtccac tttcataaag
    22261 ttcacgaatt ctttccataa tggcataggc agctaaggta tcagattttg taggttgaac
    22321 caatttgaga atatgactgg ctcgcacctc atctcttctt ttatcagtaa gttttagaat
    22381 atgatagcca ttttcacttc ggatgatccc agatatttct cctatatcca tagagaaggc
    22441 tgcatcttct ataggtttta gcatcatacc cctgctgaaa tagcctaaat caccgcctct
    22501 aactttactt gggcattcac taacctcact ggctaaagta gcaaaatctg ctcctgcatc
    22561 taatctcttt ttaatatcct gcatttgctt ataaatggaa tcttcagtgg ctttacttgc
    22621 agtaatttta tactttatca tccctgtttc ccaggtggta ggtttcacag ccattgagtc
    22681 cttagttgta ttatagaatt cacgcatctc ttcttccgta accgatacct gtgagctcac
    22741 atataagtct atcaattgct gagtcatagc attattttta atttcttcaa tgaagtattc
    22801 taataattca gcttcagtga gattttcttg cgctaaagca gcttggaact cttgaggaga
    22861 aggatattga gattttaatt cctgaagata gttttgagca tatcttttaa ttttatcgtc
    22921 atcaactttg atgtttaatt ctttagcttt ttgagagata accttttgtt caattatttc
    22981 acgtaaagcc atatccatag aaatctgttc ttttggtatc ccagccagtt gcatctgata
    23041 catcatttta taaacatcac ttaataggat aatgtctgat cccacttttg cgacaatacg
    23101 gtcaaccagc tcagctgaaa gtgagaagat gaggaatgaa gttagtagta taaggattaa
    23161 ttttttcatt atattccttt ttatttatag agattagtaa tagtagtaga tattgttatt
    23221 tttcattttg agttcacgta ctaaatcgtt gtaaacttgc tgtttttttt cttttagcag
    23281 tagttcatag atttcattac gatagtctac aaaagtggct tcttgcgttc cttctctttg
    23341 ttcagtgtag cgaatgatat aagtttttcc attaacagta actatgcctg gttcgttaat
    23401 tttaagctta tgtgctgctt tccagaacag tgaatcagga ctattagcag agacaaatcc
    23461 cattcttcct tgtttatatt ttaattcttc taaagagttt tctaaaactg cgagattaaa
    23521 atcgtattca ccggttttaa tcttatttaa caggcttttt gcagcatttt catctgggca
    23581 gagtatcctt tgaatgttat attccatcag tttttttgta aaatcagctt gatgtaagcg
    23641 ataataatta aacatttctt cttcactgat agttaaatta gccagttttc gggcaagcaa
    23701 agcattagct ttaactttct ttacggcata atctatacgc agttttgttg cattttcttc
    23761 actaattttt tctttatccg cttcttgagc cagaatggta agattaaccc agttttctat
    23821 ctcttttctt ttttgcgttt cacttaattt attccattgt tcatctgtgt aaagagagcg
    23881 aaatgcttcc aatgtaagaa cttctccatt aacttctgcc agtggagtga attcttcctt
    23941 ttcttttttt aattcacaac ttgagcagag gataaatacc attattgctg caattaaaaa
    24001 tcttttcata ctttagagaa agggtggagt gtaagtaact cttagtttag gctgtttttc
    24061 tgcaggagtg ctactatcat tgaaatgcca taattctatc tttccgtagt taattaattc
    24121 ttgcatagaa cggataacta tcccataatt tattttatcg ccagaagtat aagcttgaat
    24181 taaaggagtt acattaacta ctaaactatc tcctctgata taagattgag tgataattcc
    24241 agaagcaacc tgttcatcta agacagctac agcagtattt atatccaaag aatcattcac
    24301 tctatcagct cttaatctgt attgagtaga ggttccataa tagggatttt ctttaacata
    24361 caagataagt tctgcatgat tgatagtagt tctcttaagt tgaatctcat taagtggatt
    24421 tccctctgta tcttgaaata aagaaaagtt catagggaag tataaatata ttctggaagg
    24481 tggtatatta cttaatatcc attgattggt tattatgggt gcagtttctt catctatccg
    24541 ataactatcc ttgttgcagt attgatcata tttcttttct gtaggattct cacctgtggt
    24601 tctataaaga aaatggagct ttggtccagc acctgattcc cgtgatttaa attctatata
    24661 tgaattttct ccagttttta tagccagagt caatcccaga gaatcagctt cagaagtcca
    24721 ttgttctaaa gctgctacag gaataggtat tttaatttga gttccagctg agctaataga
    24781 atcagtgata gtatatgatt gaggagtaat taaacttaaa ttggaatcct ggataagatt
    24841 agtgctatct gctgcccagc tttgatttag tttataaatc ttaacttcaa caggattacg
    24901 agattctgaa aattttctcg taagcacgag attaagatag gtagaatcct gataagcttc
    24961 tggtatatga aatgttccca cggaaggtaa acctgtaaaa cgcacaaaag ctacggattc
    25021 tataccatta taatttccac agactaaact ggtttctgtt ccttttatag aaccactacc
    25081 aggaaaactg tagccagcta ctatatctaa aggtgtagta aaagacatag gtcttacatt
    25141 agaccagtta tttccggttg gattagggtc attaatgcaa cctcccagta taattgccag
    25201 aaataaggtt gctacgagga gtaatctcat tttttgaagt tgaatagttt tcatatatta
    25261 cctaatttat taaaatagat tttcttttat agtagtttaa gtaggcaatg tgaataagtg
    25321 gataaagggt caagtctttt tttatcttat tgaaataaag cataatatct ttagagattt
    25381 tttggggata agtagtgggt aatttcatac aaagttaagt tttttccact ctgccaggta
    25441 agacatatct tatatactaa cttatccaca ttaatccacc ggaattatcc acttatccac
    25501 ataattttaa gtttatgact ttatattttt gattagttgt tcaatatggg accggaaatt
    25561 tatatcttca cgaaagcggt tttcaatcat tcttttagcg tgaataactg tggtatggtc
    25621 ttttctttta taatattctg ctatctcttt tagagagagc tgtggtatga gcagatttgc
    25681 cagatacata gcaatttgac gtggatagga gatttgctgt cttctggttt tatcaacaat
    25741 ttgtggcatt gtaatattat aagccagaca gacttcttgg gtgatggtat caagtgtgat
    25801 ttctttatgt tgttcagata tcatatcgct aagaatagaa cggactgttt ccacatctaa
    25861 ttcttcagga ttaagattat tgtaagatgc ataggctaaa atacggatga gagaaccttc
    25921 cagagcacgg acactggaag taatggattc agcaatgaaa gaaaacacct catcactgag
    25981 aacaatattt tctggttctg cttttttatg aaggatagcc acccgagttt caaaatcagg
    26041 attcttcaaa tcgcaaagta gaccactttc aaacctggtt accagtcttt tttctaaatc
    26101 tggtatatct tttggaggac ggtcagaggt taaaactatc tgtttacgat tgtcaaatag
    26161 agcattaaaa gtgtggaaaa attcctcttg agtaccttct tttttggaaa gaaaatgcac
    26221 atcatcaaca agcagtaaat ctactttacg gtatttggaa cgaaactctg gcatcttgtt
    26281 agcacgaata gattctatca tctcattagt aaattcttca ctggtggtgt aatagataga
    26341 acaattacgt ccctctttta caacataatt tcctactgct tgcattaaat gtgttttccc
    26401 catcccactt tcaccataaa taaaaagagg attataggtg tatcctggag cttcagctac
    26461 tgctttagca gcagaatagg caaaattatt gtttcgtccc accacaaatt cttcaaaact
    26521 aaaacgctca ttgagcttgg tattgaccat aacccgatta tccgaatagc cagcggtttg
    26581 attaggattg tttttcacat aatgaggagg agaaataaac tttatcttat aatgttgctt
    26641 atacaaggca taagctatct ctcctaaagt atcggtatag ttttgattta ggtaatttgc
    26701 cgcaaattgc gttggaacct tgactacaag ttcattatcc gaaatatcca ccaactttgt
    26761 ctcagaaaac caggttctaa agctctgtgg attgatgttc tcctctaact tatctaaaat
    26821 attctgccaa atatcctgat ccatcttacc atcctaaagc taaaaaaatt tatttatcca
    26881 caaaatcagg agaggcttgt cctcagcctt aacccgattc attatttatt gctaatcaat
    26941 aagataacta tatgttaagt tcaatttgca gttttcttta gatttagtta tccacacatt
    27001 ttccacattc aattcccact tcattcactt caaactcatc ctttaatatt tactgtggat
    27061 agcttttcta aggtggaagt tcttgtcaag tggctaaatc atttaactat ttataattac
    27121 agggtttcat aatctataag ttatgaaaaa taatattatt aaaacttgaa tcttggtttt
    27181 ataaaaatcc ctggtcagaa ttctatccaa ttatcctaaa gtaaattaaa gaaattttaa
    27241 attaatttaa tctcaattag gaagaggtgt attcatttat gagctaatag atttgaaatt
    27301 ataaagatag ggatttttga ttaactcttc cataatatga agggaagaaa agttatcagg
    27361 aagaaagttc aaattttaaa attaagggaa ggaggtcgta aaatatctct attggtggct
    27421 ttttagaatt tgaatgaacc tcttccaaag tggataattg aaattagaag catatttatc
    27481 caggatgatt ttatctatct caagaatttt ttgctttagc tcaagcggta tggaatccca
    27541 ttctttttca taagataagc gagatatgac ttcaatttcg tcagctgggt catttttttc
    27601 cagataatcc ag
//
LOCUS       DAORZF010000018        27227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_1512_length_27227_cov_351.731562, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000018 DAORZF010000000
VERSION     DAORZF010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 27227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27227
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1512_length_27227_cov_351.731562"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..950)
                     /locus_tag="PK015_03385"
     CDS             complement(21..950)
                     /locus_tag="PK015_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HOL33546.1"
                     /translation="MKKCVIIICLISLFLVSCMSEQDKLEKANKNLGSKKNPIKMYFV
                     PSMEASTIVTNGEAIAKWLEKETGYRFKVAVPTSYAAVIEALGTYQADVAWLPTFAYI
                     LAKEKYDVQVKFMTIRNGLSKYRGQFVARTDSGINSLEDIEGKVIAYTDAASTSGYIY
                     PSAILQQKGIKPAKYTFAGGHPQAIAAVYNGNADVACTYWSPPDSEGRPKDAREKLLG
                     TYPDIFEKVKIIALTDSIPNDTVTFRKGLPPEVDAKLVQALTKFSQLPEGKKILRELY
                     DIDGLAPATDADYDIVRQTLKVLGKDPSAFLND"
     gene            complement(1028..2293)
                     /locus_tag="PK015_03390"
     CDS             complement(1028..2293)
                     /locus_tag="PK015_03390"
                     /inference="COORDINATES: protein motif:HMM:NF016354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HOL33547.1"
                     /translation="MSLKISAVQPRSLAHKAGIKKGDTIISINSMPINDFFDLEYYSN
                     DYQLDFELLDTEGEPKFITIERVNGKTLGIEPEPHNVRICHNHCIFCFVDQMPPKMRH
                     SLYVKDDDYLFSYVFGNYITLNNLESMDINRIVNQHISPLYVSVHTTNPALRQKMMRY
                     KENYDIVKTLMELSQKGIEFHLQIVAVPGYNCGEELEKTLDTLLNESIAALSIGIVPV
                     GLTGFREHLTPLSPFNSDLALETLKIIDRYREKSPIIYGADELFLLTETPIPEADYYG
                     DFPQLENGIGMLRLTLMNYEKKRKSFIKELDKAGGNFLLLTSTLANTILQEIADDLNN
                     HLKQAKVKVQPIRNNFFGGYVGVSGLLTASDILSQVQPLPQESIIIPENLFNTDGLTL
                     DDVSQLELHDKLQVPILIVDPYFEDWEWI"
     gene            complement(2304..3434)
                     /locus_tag="PK015_03395"
     CDS             complement(2304..3434)
                     /locus_tag="PK015_03395"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOL33548.1"
                     /translation="MTKIKYILLLLSILWVGYLQADITLVLTPNGTQQSLPVVKLESK
                     DYVSLDEINTRLYSIYKVEYSEFTDFRVMFFFYGEQFIFLLNSPFYTFKQSIFNMQYP
                     LLQVGPNYFVPTTFIMDNLPLHFPGQIQNKNGVLYLPQPKDKSVKRIVIDPGHGGKDP
                     GAIGPNGTRESHINLNVALRLKQLLEQELGLEVLMTRTDDRFVSLGDRTKFANDNKAD
                     LFISIHTNASRTNGAQGIETYYLSTKSTTESRAVEALENSVVELYEGAGAKQKYEALD
                     FILMDMLQTEHLEHSNNLATLVQHNLVAGTQTKNRGVKQANFYVLRGAFMPAILIEMG
                     FISNPEEEALLANPKYQDRIAQAIFEGIKRFKYDYDRIRLTS"
     gene            complement(3427..4377)
                     /locus_tag="PK015_03400"
     CDS             complement(3427..4377)
                     /locus_tag="PK015_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOL33549.1"
                     /translation="MNLQYPQLINCQVCPQNCRVNRYETTGFCKADYRLKINLAQLHH
                     GEEPVFSGTNGSGTIFFSYCNLRCVFCQNYPISHLGWGTYYDEEECAQIMLKLQEAGA
                     HNINLVSPTQYTPQLVQTIKIARENGLSIPILWNSNAYENVSTLQSLAGLVDIYLPDF
                     KYAHSFYSKKYSSAKDYPEIAIIALEEMFRQVGHLQLDSEGIAQKGILVRHLVLPNHL
                     SSTKQALYILYEHFGPELALSLMAQYYPAGRANEFPELNRSLKREEYEEAVETAEALG
                     FKNLYLQKFYSSPEWTPDFQPHNEVFDEKKLHFQGKKRND"
     gene            complement(4374..4766)
                     /locus_tag="PK015_03405"
     CDS             complement(4374..4766)
                     /locus_tag="PK015_03405"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOL33550.1"
                     /translation="MNIDFDLLLDQAKSASEKAYAPYSGYKVGAALLCSDGTVFTGCN
                     VENASYSLTICAERNAVFQAVAAGQRDFLALAIYVDGEVLFPPCGACRQVLAEFNPNL
                     HILYANRLGSILSDLDTLLPQAFTLSEI"
     gene            complement(4763..5872)
                     /locus_tag="PK015_03410"
     CDS             complement(4763..5872)
                     /locus_tag="PK015_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOL33551.1"
                     /translation="MSTLFGGILALQWQQLVMILIGLVLIYLAIAKKYEPTLLLPIGF
                     GTILTNIPLSTAIGPHGPLQVLFDAGIATELFPLLIFIGIGAMIDFTPLLKNPSMLLF
                     GAAAQLGIFVTLAVATLLGFDLKQAGAIGIIGAADGPTSIYVANRFARELLPAISVAA
                     YSYMALVPIIQPPIIKLLTSREERKIHMDYHSGDVPQVIKIVFPIAITLIAGIFVPAS
                     VALIGFLMFGNLLRECGVLDNLAKSAQNELANIVTIFLGLSIASTMKGEAFLRPQTLL
                     IFVLGLIAFIFDTAGGVLFAKLMNLFRKHNKINPMIGACGISAFPMSARVVHQMGQKE
                     DPFNFLLMPAVSVNVGGQIASVIAGGLILALLGNL"
     assembly_gap    6069..6168
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6169..8498)
                     /locus_tag="PK015_03415"
     CDS             complement(<6169..8498)
                     /locus_tag="PK015_03415"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOL33552.1"
                     /translation="MKIKQITTLLLTLLICQISLGNHLSEIDSQITKGITNSSETLKA
                     STPLAEDLFISEYVEGSSNNKALEIFNGTGHPVNLSDYKVQIFYNGAQNPGANINLNG
                     ILNNGDVFVIAHSSASSDILKIANLSHGSLSFNGDDAITLTKISTNSYVDIFGVIGND
                     PGNAWIGPNGYSTKDKTLVRNPEVCHGVTINPSGTGPNAFTTLTTEWTLFDRDTFTNL
                     GFHLFTGNNTEVDPPTIQTSNITGYPGVTSMTLEWTPGNGSRRIVKMNTVNSFTDPLD
                     GSYPVANPIWANSGEQVIYNGSTQIVEDTPFNGCEVTNLNPSTTYWFRAYEYNGTGSN
                     TKYLTTTATGNPASLTTLEGQGSGYYAGIDGYGANLKQQLHTLIKDTHTTKYSYAALI
                     TQIPYTDEDPENSDNLIEIYTGWSVNKNNFGNATTDWNREHTWSKSHGNFGDTPPAGT
                     DLHHLRPCDSTVNSTKGNKDFAEGGTPVIDNSPPAGYTGETGCFQTTYSWEPRNDDKG
                     DVARMIMYMAVRYEGDDPNFQTNLEMVDYIHSDENTNQPYYGKLSTLLQWHIIDPPDE
                     REMQRNNRIAERQGNRNPFIDHPEYATRIWTPCPIANTNITETSFTANWSQPIQATAY
                     YFQLASDSLFINILPGYENLNVQLTTSKTITGLNNGSTYYYRLRSFFLEGYGMWSPFM
                     SVTLTSPEPITATLSSSFPLDEYNLNGAFLTLVLTGTTFTDNNLLVSNFTLNNAPAGL
                     TIQSVNYINPTTAIITLNFNGLDFDINYYNVSITIAA"
     gene            8711..10447
                     /locus_tag="PK015_03420"
     CDS             8711..10447
                     /locus_tag="PK015_03420"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOL33553.1"
                     /translation="MSKIRLSVLIPLLLLAFIPAMYAFESTMSLEGGINFPQDDVEGD
                     NVLMGSWAASWDLWCFGDLGIGINPWFTNLKVKNGPNDYHSSLEGVNLYLKFKPTKGV
                     AENFPRDYTINRVSPFIALGAGWSTHNSEDEYGNIDDASPDKIGGHLTFPYASIGISL
                     LSKWNTTLDLGLKYTMFASDKIDLVKDGDFNDALLTPYIAVGMHFGRKEPLPKKIDPE
                     IVKLRKDLNATDLRLNEVIEQLNAVTLQLPELQKLQMSIEQSETNIATLQTQYAQMNI
                     KVTELTNKVNNLEQRVAQVPTQKTPSADIYANEIKQIKDTQSALAANMATKEDIAKLE
                     QEKAEIAITVNQINNELAEITKKLEQLDALTAQVNAIKGAADVSAYTKELEELRNMLN
                     NYEKELAAIRTDTENLHKADNDLAANINDLRSRLLTLSDELKKLSEKPKPVVTPEPQV
                     QEKPQVIEKDYLTELEEELKGKMIHFATNATTLSEKDKLFLDKVAEYLIKLPEVKIEV
                     QGHTDNTGSDKINNTISYNRAKSVADYLIAKGVPESQLIVKGYGPSKPIASNKTAQGR
                     ATNRRVQLVIVK"
     assembly_gap    10593..10692
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10693..11517
                     /gene="pgk"
                     /locus_tag="PK015_03425"
     CDS             <10693..11517
                     /gene="pgk"
                     /locus_tag="PK015_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOL33554.1"
                     /translation="WASFADLYINDAFGSWQAHASTYNITKFLPSYAGLLMIKEVQNL
                     EKVFNPQRPLLSVVAGAKFDTKMGPLSALLKLSDKLVLGGVLYNGYLAAKYKVNIKGL
                     SPEDLQLAEQFLQEAEKFPDKIVEMPFIIECDSMEDVSNWRIRKVTDLQAGMDLNYIL
                     DIAPESFSDSSIQEVFSSAATIFVNAVMGYTVLFKEGTKALYSLIHSCSQAQKLFGGG
                     DTIQEFGDLLPGIFAQAKNDPRYYFFTGGGAVLDAIAQGSPYGMKPVQALLQKIEK"
     gene            11530..13434
                     /locus_tag="PK015_03430"
     CDS             11530..13434
                     /locus_tag="PK015_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOL33555.1"
                     /translation="MEWEKELKLLEDFPPPTYEEWRKAVEDTLKGADFDKVMHTRTYE
                     GITLQPIYRREDLSKLPFLDSLPGKAPYVRGNNPKKFLSEGWLIAQSHRESDLKALNA
                     QILRELQLGLTAVNIQLKHDDDLRGVSIKSIDDLRAICAAIDLRAAPLFMQLDMDDYF
                     ILLLVEQYICERKYAWEELQLGVGFDPISEFARKGYLSFSWDEVWGKIVEGVEIAIKN
                     ISGLRCISIDGSVYSAAGASSTQELGIILATAIYYLQNLLDKGWTIEQLSPLFQVKLS
                     LGPNFFMEIAKVRAMRLLWSEMIKAFGGKEKEQKIWIHGKTATFNQSLYDVYVNLLRN
                     ATEAFSGVIGGVDSLEVGRFNELTSEEDEFSRRLARNQQIILKEESHFDKVVDPAGGS
                     YYIENLTQELANQAWKYMQELEAEGGILNALQEGKIHTDIEKVANARKEAMRKRKDVY
                     VGINLYANPDENPPTQIQIPRESNPIKMEVLEAGALPQLRVVEEIENLRTRVIKSDKN
                     TNIFLMNMGTINDYRVRADFATGFFQAGGFTVISPQGFMTVEDAVKSAKESNAAAYCI
                     CSTDEKYNELVPAICSALPDSFLILAGYPKDKVESFKENGIKMFIYMGADVVATLDEL
                     AKKLGVENEA"
     gene            13424..15550
                     /gene="scpA"
                     /locus_tag="PK015_03435"
     CDS             13424..15550
                     /gene="scpA"
                     /locus_tag="PK015_03435"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HOL33556.1"
                     /translation="MKPDFTKINPEFKVEAKSKPEELPIWKTNEQIEVKGLFTKDDIA
                     QAQHLDYLSGLPPFLRGPYPSMYILRPWTIRQYAGFSTAEESNAFYRRNLAMGQKGLS
                     IAFDLPTHRGYDSDHPRVAGDVGKAGVAVDSVLDMKILFEGIPLDQISVSMTMNGAVI
                     PIMAFYIVAAEEQGVKPEQLNGTIQNDILKEYMVRNTYIYPPEASMRIIADIFRYTAE
                     HMPKFNSISISGYHMHEAGATADLEMAYTLADGLEYVRTALKAGLDIDVIGPRLSFFW
                     AEGMNYFMEVAKLRAARVLWAKIIKQFLPQNPKSMALRTHSQTSGWSLTAQDPFNNVT
                     RTCIEALAATMGHTQSLHTNSLDEAIALPTDFSARIARNTQLYLMNETGITRVIDPWA
                     GSYYVEYLTDALLKKSWEHILEVEELGGMAKAIETGLPKMRIEEAAARRQAHIDSGEE
                     TIVGVNKYCPENEEPIEILEVDNTAVQEAQIARLKKLKAERDSQKVQACLDAITEAAK
                     NNTGNLLELAIEAARARATLGEISYAMEKVFGRHQATIQLINNVYSSEYTKMDEIEKV
                     RALTDKFAEKEGRRPRILVAKLGQDGHDRGAKVVSTAFADMGFDVDVGPLFQTPAEAA
                     RQAVENDVHIIGVSSLAGSHKTLLPQLVEELAKLGRDDIMIVVGGVIPPQDYDFLLAN
                     GATAIFGPGTKLPEAATIIMNKLLQE"
     gene            15552..16652
                     /gene="meaB"
                     /locus_tag="PK015_03440"
     CDS             15552..16652
                     /gene="meaB"
                     /locus_tag="PK015_03440"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOL33557.1"
                     /translation="MTKERKPEWTPPNAGKEYASRVLKAKANQSGIVKETGKKRSSFP
                     SVDELVEGIKKGDRTLLAKAITLIESNAPQHFEAGQELIRRLLPDSGNSIRIGVTGMP
                     GAGKSTFIENFGLWLLGKGHRVAVLAIDPSSTISRGSILGDKTRMEELSRHPDSFIRP
                     SPSGGTLGGVARKTRETIILCEAAGFDIVLIETVGVGQSEVTVRSMVDFFLLMQIPGA
                     GDELQGIKKGVMELADLVVVNKADGENIAAAELACRELNNALHYLRPATEGWTTKAIT
                     CSALQKKGLSEIWEIIEKFVEQGKSSGIFYERRKKQVLEWFESLIKEAVLNRFYSDHK
                     VQEKLPLLKKQVEEGSLPVMLAVQQLLNVYED"
     gene            complement(16649..17125)
                     /locus_tag="PK015_03445"
     CDS             complement(16649..17125)
                     /locus_tag="PK015_03445"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HOL33558.1"
                     /translation="MIPLVETRFNIVLYEPEIPANTGNIGRLCVGTGSKLHIIQPCKF
                     LITDKTLKRAGLDYWEHLDLTMHKTWEEILEFCPLERVFFCTTKSKRNYLNEHYQYGD
                     TFVFGPESRGLPQNLLDKYPERLITIPMHPLIRSINLANSVAIILYEAIRQVYYSS"
     gene            complement(17128..18204)
                     /locus_tag="PK015_03450"
     CDS             complement(17128..18204)
                     /locus_tag="PK015_03450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA];
                     GO_process: GO:0030974 - thiamine pyrophosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HOL33559.1"
                     /translation="MTLRKRSVKLFIIISLVLCLISGFSCKKNNKEVKKQITPSSYLY
                     IFALDHIRESGFETVVIKDFAKQNNIGLRINLFPDLSMLMQTLSNPDYQGKVDIVMGL
                     DNSFFYDIASSDYFAPVPEVSLQEIRYEIPRDSEHCLIPYASANLAFIYDSRKYPEPP
                     HSFGELQDSKYYSQLAICDPSQCGLGRSTLLWTVALFSEQGYEQLWSSLRKNIYKIYP
                     NPTLCLDAIRTGKCGLMFGYNTIPAWLNELYPSESHIKSIIPEEGSFLYVESAALCKD
                     APHKDTALQFLKYLISPNPQQFVMYKLAMMPVNGRTPLPTSFAHIPAIIYSHNNRLEQ
                     QPVQDSLQVWMSNWKRMMYGNIRF"
     gene            complement(18201..18953)
                     /locus_tag="PK015_03455"
     CDS             complement(18201..18953)
                     /locus_tag="PK015_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOL33560.1"
                     /translation="MNFKLLAGTGAYTIFIGRVLKRLPKLPKRWQELTIQLKRIGVDS
                     LFLITITSIFTGLVVAVQIIYQGSYYIPKNLFSVIIGKANMIELAPVLTGLVLTGKIG
                     ASIAAEIGTMRVSEQIDALQSMSIAPEEFLYLPRISAGVISAPLLTIYADLISIIAGW
                     FFTWMRYGIRFFNFFNDMRIYFAPFDLIGGIIKAILFGFLITSLACFFGDKTTGGAEG
                     VGHSTMQTVVYSSVGVLVLDFLVAILMMAHYL"
     gene            complement(18943..20319)
                     /gene="dnaB"
                     /locus_tag="PK015_03460"
     CDS             complement(18943..20319)
                     /gene="dnaB"
                     /locus_tag="PK015_03460"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOL33561.1"
                     /translation="MAKKETIPKEITQISDRALPADVNAEAGVLSAMIIDSSIVSKGL
                     ELIKEEYFSRTSHKLIFRTIAELFSDGVEIDPLTLVDRLQQKGFLEKVGGIPYINELS
                     DFVTSSANFNYHLNILIEKALLRNLIITCNSIIEACYSSTEPVKKIVDEAEQAIFKIA
                     EMPQHQGFQRIDQISAEVIQVIDTISTTKVPVIGVSSGFIDLDRLAGGFRPGQFIIIA
                     ARPAMGKTSLALNIASHAAVDLGKKVAIFTMEMAADEVVMRMFSSAAEVSMESILKGY
                     GMNEEKLIRIMQVADVISTKQIYIDESGTNTPLEIRAKTRRLASEIGGLDLIIIDYLQ
                     LMTLPRDKDNRQQEIAEISRALKILAKDMKIPVIALSQLNRLLENRDDKRPRLADLRE
                     SGAIEQDADLVMFIYRDEYYTKEKSEKPGIAEIIIGKNRHGSTGTVELGFNKEFTMFR
                     NLDTIHEF"
     gene            complement(20321..20806)
                     /gene="coaD"
                     /locus_tag="PK015_03465"
     CDS             complement(20321..20806)
                     /gene="coaD"
                     /locus_tag="PK015_03465"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOL33562.1"
                     /translation="MSTNAIYPGTFDPITYGHINILEKASRLFDKVILSVAAYTDKET
                     LFSLQERYELCLESVKHLSNVEVYTFEGLAVDFARSQNCSVMIRGLRAVSDFEYELSL
                     ALTNTTLNPEIETVFLVPSLRYMYLSSSITRQLAELGSELKDFVPPVVAKALRDKFKK
                     E"
     gene            complement(20796..22034)
                     /locus_tag="PK015_03470"
     CDS             complement(20796..22034)
                     /locus_tag="PK015_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOL33563.1"
                     /translation="MDADIAKKRREKLSEQLGDNESVIVFAKPEITLEKFSQDSNFLY
                     LTGINEPELIYIAAKVSGRWNEVLFIERGNPERVVWEGAKLSMEEAKEISGIQQIRYK
                     DEFYPYLSGMCSQLSRIYSNLGYPMLHKPATYPLFMLQPVREKYPHIEIRDLGDLITP
                     LRLVKDEWEIEQLKRAIDITGLGIMDILHTAKAGMMEYELEATLFYRMQVNGLQQWGF
                     APIIATGINAATLHYEKNNCQIQPGDLILMDVGASCNGYSADITRCFPIEQRFSYRQA
                     EIYNIVLDVNKRIIELVKPGVELSVLNNKTVEWLAEGMLSIGLINSKDEVRKYYMHSV
                     SHFLGLDTHDVGGREAVLTPGNVITVEPGIYIPEERLGVRIEDDVLVTDDGHEVLSAG
                     IPKEISEIESIRQEAFQNEH"
     gene            complement(22139..23380)
                     /gene="clpX"
                     /locus_tag="PK015_03475"
     CDS             complement(22139..23380)
                     /gene="clpX"
                     /locus_tag="PK015_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOL33564.1"
                     /translation="MERYKNLTCSFCGRNETEVKTLIRGIAGNICDECITMCHNIMVS
                     NAPEPEEEIIDLPKPSEIKAYLDQYVIGQEEAKMIISVAVYNHYKRIFSRKDDDIDLE
                     KSNILMIGPTGCGKTLIAETLARFLKVPFAISDATTLTEAGYVGEDVENILVRLLQAA
                     DYDVAKAEKGIVYIDEIDKISRKSETPSITRDVSGEGVQQALLKILEGTKVNVPPKGG
                     RKHPQQDFIEMDTKNILFIAGGAFFGLEKIIKERIDKKAIGFDVKLGTNIDEANIFDQ
                     TISQDLLKYGLIPELIGRMPVLCTLHELTEEALVEILVKPKNALCSQYKKYFELDGVE
                     LEFGEDALREIARIAINQKTGARGLRSIMEKFMLKIMYNIPDLDNIRSCHITADVVSK
                     GAEPEFVFSSKSSSKRAAITR"
     gene            complement(23454..24038)
                     /gene="clpP"
                     /locus_tag="PK015_03480"
     CDS             complement(23454..24038)
                     /gene="clpP"
                     /locus_tag="PK015_03480"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOL33565.1"
                     /translation="MSYIPIVIEQVGRTERAYDIYSRLLKDRIVFVSGIIDDNLANSV
                     VAQLLHLEGEDPERDIYMYINSPGGIISSGLAIYDTMQYIKPKVSTICIGQAASMAAV
                     LLAAGSPGKRTALPNSRIMIHQPLGGFEGQASDIEIQAKEILYLRERMNQILHSHTGQ
                     DIKKIREDTERNYYMDAMEAKNYGIIDEVIDHRK"
     gene            complement(24035..25390)
                     /locus_tag="PK015_03485"
     CDS             complement(24035..25390)
                     /locus_tag="PK015_03485"
                     /inference="COORDINATES: protein motif:HMM:NF017508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOL33566.1"
                     /translation="MQTEIKPINEVCSEVTVVIEAEKVDEAYKKYFNKKAQELEIPGF
                     RKGKAPKNMIEKLHGADIKEDFEIKFAIDSLYQVLRENNLNFLATPQLKSLNWNQGSD
                     MKLTVILEHIPDLTLDLLENLEVPFKPQTLEDAVNQFLSDLRWKQRTIQDVDTVEENT
                     TLQCRLYFTWKGEDYNYSVNIYSEELENLPALKELISKKIGDKVSLSLPIETLKQFID
                     DDTIDFEDETQMDVTIEIDAITKLIIPEIDDEFAKDMDFENLEEMKAKIGEELKAQVE
                     HSNYEAQNNAIINALFKKKPFKIPPMTLKSIIDDYAASLHIDENHPLYNYYVNEAANY
                     LVRYLIVDSLREKIHIEVSEDMINEFIEHRAILSNSSVAGYKEKNEDYIKSDNFIRDA
                     QDYFLLRKIAETCTFIEPTGETEYPAYEIAPYEPEEEEEQDKPEEQVEPSEENVRKEE
                     Q"
     gene            complement(25409..25494)
                     /locus_tag="PK015_03490"
     tRNA            complement(25409..25494)
                     /locus_tag="PK015_03490"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25458..25460),aa:Leu,seq:tag)
     gene            complement(25528..26367)
                     /gene="panC"
                     /locus_tag="PK015_03495"
     CDS             complement(25528..26367)
                     /gene="panC"
                     /locus_tag="PK015_03495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOL33567.1"
                     /translation="MQIYKTIAEIREALAGKALQKKIGFVPTMGALHKGHLSLVERCH
                     SESDLTVVSIYVNPTQFGPQEDLNHYPRNLEKDIDLLRNYHVDIVFTPDDVQMYPEGY
                     CTWVQVEGLSDILCGASRPGHFRGVATIVLKLMHIVNPKLMFMGMKDYQQIIVLEKMI
                     EDLNLETKIVRCPIVREPDGLAMSSRNSYLTPDERQRATCLIKALKNAQQMVNEGYLD
                     SSILISEAENIIKQSDGRIDYIKIVDGSTLQELPEVRSGSRMMLAVYIGNTRLIDNIE
                     LLP"
     gene            complement(26367..26717)
                     /locus_tag="PK015_03500"
     CDS             complement(26367..26717)
                     /locus_tag="PK015_03500"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOL33568.1"
                     /translation="MLRKMLLSKLHRATVTECNMHYNASIKIDQELLKLSGMREYEQV
                     EVWDINNGERFSTYIIPGEPGSRTIGIYGAAARKVQVGDIVIIVNYGLMDEEELDSYQ
                     PCIILLDEDNNPLP"
BASE COUNT         8494 a         5018 c         5026 g         8489 t
ORIGIN      
        1 agctgtgaca ttacctcttc tcagtcattc aggaatgcac ttgggtcttt accaagcact
       61 tttaaggttt gacgcacgat atcataatct gcatctgttg caggtgcaag accatctata
      121 tcatagagtt cacgcagaat cttttttcct tctggtagct gagaaaattt agtcaaagct
      181 tgaaccagtt ttgcatctac ttctggtggg agtcctttcc ggaaagtgac agtgtcatta
      241 ggaatagaat ccgtgagagc aatgattttt actttttcaa agatatcagg ataagtgccc
      301 agaagttttt cccgtgcatc tttgggtctg ccttccgaat ccggaggtga ccagtaagtg
      361 caagcgacat ctgcatttcc attatataca gcagctatgg cttgaggatg accaccagca
      421 aaagtatatt tagcaggttt aattcccttt tgctgaagta tagcggaagg ataaatatat
      481 ccagaagttg aagctgcatc ggtataagca ataactttgc cttctatatc ttccagacta
      541 tttattccac tgtctgttct cgctacaaat tgacccctat atttgcttaa accattccgg
      601 atagtcataa attttacctg gacatcatat ttttcttttg ccagtatata ggcaaaagta
      661 ggaagccagg caacatctgc ttgataagta ccaagagcct ctattactgc tgcataactg
      721 gtaggaactg caaccttaaa acgataaccg gtctcttttt ccagccactt tgctattgct
      781 tcaccattcg taacaatggt agaagcttcc atagaaggga caaaatacat cttgatagga
      841 ttcttttttg agccaagatt cttatttgcc ttttccagtt tgtcctgttc actcatacag
      901 gaaaccagaa atagacttat aagacaaatt attataacac attttttcat atgaaacatc
      961 cttacacaag agttatggtt ttttctttat aatttataat atttaagttc ttagcataca
     1021 tagctcttta aatccattcc caatcttcaa aataagggtc aactattaga atcggaacct
     1081 gtaatttatc gtgaagttcc aattgagata catcatccag agttaaacca tctgtattga
     1141 aaagattttc agggataatg atactttctt gaggaagagg ctgaacttga ctcagaatat
     1201 ctgaagcagt aagtaatcca cttacaccaa catagccacc aaagaaattg ttcctgatag
     1261 gctggacttt tacctttgct tgcttgagat gattattgag gtcatcagca atttcttgaa
     1321 gaatagtatt agccagagtt gaggtcaata acaaaaaatt tccccctgct ttatcaagtt
     1381 ctttgatgaa actttttctc ttcttctcgt agttcattaa agttaaacgc agcattccaa
     1441 taccattttc cagctgcgga aaatctccat aataatcagc ctcaggaatt ggagtttctg
     1501 taagaagaaa tagttcatct gctccataga taatagggga tttttctcta tatctatcaa
     1561 tgattttcag agtttctaat gccaggtctg aattgaaggg agaaagcgga gttaagtgtt
     1621 ctctaaatcc agttaaacct actggaacaa tacctataga tagagcagca atagattcat
     1681 tgagtaaagt atcaagagtc ttttccaatt cttctccaca gttataacct ggaactgcta
     1741 caatctgcag atgaaattct ataccttttt gactgagttc catcagagtc ttcactatgt
     1801 cgtaattttc tttatagcgc atcattttct gtcgtaaggc tggatttgtg gtatgtacgg
     1861 aaacatataa ggggcttata tgctgattca ctatacggtt aatgtccata gactcaagat
     1921 tattaagagt gatataattt ccaaaaacat acgaaaaaag atagtcatca tctttaacat
     1981 aaagtgaatg cctcatttta ggtggcatct ggtctacaaa acagaaaata caatgattgt
     2041 ggcatatgcg aacattatga ggttctggtt caatacctaa ggtctttcca ttgactcgtt
     2101 caatagtgat aaatttgggt tcaccttctg tatccagcaa ttcaaaatcc agttgataat
     2161 catttgaata atattccagg tcaaagaagt cattaatggg cattgaatta atgctgataa
     2221 tagtatctcc tttttttatt cctgccttat gagcaagact tcgtggttga acagcactaa
     2281 tctttaaaga cataatgaat agattaagag gttaaacgaa tacggtcata gtcatatttg
     2341 aagcgtttta tgccttcaaa gattgcttga gctatgcggt cttggtattt tggattagcg
     2401 agcaatgctt cttcttctgg attggaaata aaacccatct caattaagat agcaggcata
     2461 aaagctcctc taagaacata aaaattagct tgctttacac ctctattttt ggtttgagta
     2521 ccagccacaa gattgtgttg aacgagggtg gctaaattgt tactatgttc cagatgttca
     2581 gtttgaagca tatccatcag gataaaatcc agagcttcat atttttgttt tgctcccgca
     2641 ccttcataaa gttctaccac actattttcc agagcttcca ctgctctact ttctgtggta
     2701 gatttggtag aaagatagta ggtttctatt ccttgtgctc cattagtacg agaagcatta
     2761 gtatgaatac tgataaagag gtcagcttta ttgtcattag caaattttgt cctatctccc
     2821 agagaaacaa aacggtcatc tgttctggtc atcaaaactt ccagacccag ttcttgttcc
     2881 agtaattgtt ttaaccttaa agcaacatta agatttatgt gtgattcacg cgtgccatta
     2941 ggtcctattg caccagggtc ctttcctccg tgtccagggt caatcacaat tctttttaca
     3001 cttttatctt tgggttgagg caaataaaga acgccatttt tattctggat ttgacctgga
     3061 aaatgcaaag gtagattatc cataataaat gtagtcggaa caaaataatt aggtccaacc
     3121 tgtagtagag gatattgcat attgaaaatg ctctgtttaa aagtgtaaaa aggagaattt
     3181 agcagaaata tgaactgttc tccatagaag aaaaacatca ctctgaaatc agtgaattcg
     3241 ctatattcaa ctttataaat tgaataaagt cgggtattta tctcatcaag tgaaacataa
     3301 tctttagatt ccaattttac cactggaaga gactgctgag ttccattagg agttagaaca
     3361 agtgttatat ccgcttgtaa ataacccacc cataaaatag ataatagaag aaggatatat
     3421 ttaattttag tcatttcttt tctttccttg aaaatgcaac ttcttttcat caaagacctc
     3481 attatgaggt tgaaaatcag gtgtccattc gggagaactg taaaactttt gtaaatagag
     3541 atttttaaaa ccaagagctt cagctgtttc tacggcttcc tcatattctt ctctttttaa
     3601 acttcggtta agctctggaa attcatttgc tctacctgca ggataatatt gtgccataag
     3661 actgagagca agctcaggtc caaaatgttc atacaaaata taaagcgctt gtttagtgct
     3721 ggacagatga ttgggaagta ccagatgacg aaccagtata cctttttgcg caattccttc
     3781 ggaatcaagc tgaagatgtc ctacctgcct gaacatttcc tctaatgcta taatagctat
     3841 ttcaggataa tctttagctg acgaatattt tttactgtag aagctatgag catacttgaa
     3901 atcaggaagg taaatatcta ctaaacctgc aagagattga agagtggaaa cattttcgta
     3961 agcattgcta ttccagagta tagggatact taatccattt tcacgagcta tttttatggt
     4021 ctgcactaat tgaggagtat attgagttgg agaaacaaga ttaatattat gagctcccgc
     4081 ttcctgcaat tttaacatta tttgagcaca ttcttcttca tcataatagg ttccccaacc
     4141 taaatgagaa atgggataat tctgacagaa aacacaacgc agattacaat aggagaaaaa
     4201 gatagttccg cttccattag taccgctaaa aacaggctct tctccatgat gcagttgagc
     4261 aagatttatc ttcaatctat agtctgcttt gcaaaaacca gttgtttcat aacgattgac
     4321 ccgacaattt tgtgggcaca cctgacaatt gattaattga gggtattgaa gattcatatc
     4381 tccgacaggg taaaagcttg aggcaacaaa gtatccagat cgctgagaat actgcctaaa
     4441 cgattagcat aaagaatatg gagatttgga ttaaattcag ccagcacctg acgacaggct
     4501 ccacaaggtg gaaataatac ttcaccatcc acataaattg ccagagctaa aaaatctctc
     4561 tgtcctgcag ctacagcttg aaatactgca tttcgttctg cacaaatcgt caaagaatag
     4621 gaagcatttt ccacattgca accagtgaag acagttccat cagaacagag taatgcagct
     4681 ccaactttat agccagaata aggtgcatag gctttttcgg atgcgctttt agcctgatcc
     4741 aagagaaggt caaagtctat attcataaat tccccaataa agctaaaatc aatccacctg
     4801 caataactga agctatctgt ccaccaacat taacactaac ggcaggcatt aaaagaaaat
     4861 taaaggggtc ttctttctgt cccatttgat gaactacccg agcagacata ggaaaggcag
     4921 aaataccgca agctccaatc atcggattga ttttattatg tttacggaat agattcataa
     4981 gcttggcgaa aagaactcct ccagctgtat caaagataaa tgctattaat cctaaaacaa
     5041 agattagtaa agtctgagga cgtagaaaag cttcgccttt catcgtggaa gcaatgctta
     5101 agccaagaaa gatagtgacg atatttgcca gttcattttg agctgatttg gctaaattat
     5161 ccagaactcc acattctcgc aagagattac caaacattaa aaaaccaatt aaagctactg
     5221 aagcaggaac aaagattccc gcaatcaagg ttatagcaat agggaagacg atttttatta
     5281 cctgaggaac atctccagaa tgataatcca tatggatttt tcgctcttct ctggaagtta
     5341 aaagtttaat tattggcggc tgaatgatag gaactaatgc catataagaa taagcagcaa
     5401 ctgatattgc gggaagaagt tcacgagcaa aacgattggc aacataaatg gaagtaggac
     5461 catcagctgc tccaattatt cctatagcac ccgcttgttt caaatcaaat cctaaaagtg
     5521 ttgctacggc aagagtaaca aaaattccca gttgagctgc agcaccaaaa agaagcatag
     5581 aaggattttt tagtaaaggg gtgaaatcta tcatagcacc aatgccaata aagattaaaa
     5641 gagggaaaag ctcagtggct attccagcgt caaataaaac ttgaagggga ccatgtggac
     5701 caatagcagt agataatggt atatttgtaa gaatagtgcc aaaaccaata ggaagaagta
     5761 aggtcggttc atatttcttt gcaatagcca gataaatgag caccaatccg attagaatca
     5821 ttaccaattg ttgccattgc aaagctaaga taccaccaaa taatgtgctc atcagcatta
     5881 ttcccctttt attttattta aaacataatt aaagtttcta ctcacttgtc aatgataaaa
     5941 gtgctatcag aattatttcg ctcggtttaa agcagagtta attcttttat tttgagtgga
     6001 tagctaaagt ttattgcctc atagctgcca ctttgaagaa atatctgtct tctggcaaag
     6061 cctgaaagnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc agctatggtt
     6181 atactaacat tatagtaatt tatatcaaaa tcaagtccat taaaattcaa ggtaataata
     6241 gctgtggtcg gattgatata atttacactc tgaatagtta gtccggcagg agcattattt
     6301 aaagtaaaat ttgacactaa tagattgtta tcggtgaagg tagtgcccgt taaaacaagg
     6361 gttaggaaag ctccattaag gttatattca tcaaggggga aggaagacga aagcgtagcg
     6421 gtaataggtt ctggtgaggt taaagttact gacataaaag gagaccacat tccataacct
     6481 tctaagaaaa aggaacgtaa tcggtaataa taggtagaac cattattgag accggttatt
     6541 gttttacttg tagttagttg aacattcaga ttttcatagc ctggtaggat attgataaat
     6601 aagctatcgg aagcaagctg aaaataataa gctgttgcct gaataggttg actccagtta
     6661 gcagtgaagc tggtttcagt tatattagta ttagcaatag ggcagggagt ccaaattctg
     6721 gtggcatatt ctggatggtc aatgaaggga ttacgattac cctgtctttc tgctattcta
     6781 ttatttcgtt gcatttctcg ctcatcaggt gggtcaatta tatgccattg aagcagggtg
     6841 gatagtttcc cataataagg ttgatttgta ttttcatcgc tatgaatgta atctaccatt
     6901 tccaaattag tctgaaaatt agggtcatct ccttcatatc taacagccat atacattatc
     6961 attcttgcaa catcaccttt atcatcattg cggggttccc aggaataagt ggtttggaaa
     7021 cagccggttt ctccagtgta accagcaggt ggggaattat ctattaccgg agttcctcct
     7081 tcagcaaaat ctttattacc cttggtgcta tttacggtgc tgtcacaagg gcggagatga
     7141 tgtaaatctg taccagctgg aggagtgtca ccaaaatttc catgactttt actccaggtg
     7201 tgttctctat tccaatcagt ggtagcattg ccaaaattat ttttattaac actccatccg
     7261 gtatagattt caattagatt atcactgttt tcggggtcct catctgtata gggtatctgg
     7321 gtaatcagag cagcataaga atatttggtg gtatgagtat ctttgattaa agtgtgtagt
     7381 tgttgcttta aatttgcacc ataaccatca attcctgcat aataaccaga accttgccct
     7441 tctaaagtag taagtgaagc aggatttcca gtagccgtgg tagttaaata tttagtatta
     7501 cttcccgttc cattatattc ataagctcta aaccagtaag ttgtggaagg attaagattc
     7561 gttacttcac agccattaaa gggagtgtct tccactattt gagtgcttcc attatagata
     7621 acctgttctc cagaattagc ccagatggga ttggcaacag gatagcttcc atccaaagga
     7681 tcagtaaaac tattaacggt attcattttt acaattcttc tggaaccatt tccaggagtc
     7741 cattccagag tcatagaagt aacgcccgga tatcctgtga tattggaagt ctggatagtt
     7801 ggagggtcta cttctgtatt atttccggtg aaaagatgga atccgagatt ggtaaaagta
     7861 tcacgatcaa acaaagtcca ttcggtagtt aaggtggtaa atgcattagg acctgtacca
     7921 gaaggattga tggttactcc atgacaaacc tcaggattac gaactaaagt cttatctttt
     7981 gtggaatatc cattaggacc tatccaggca tttccaggat cattacctat aacaccaaat
     8041 atatcaacat atgaattagt gcttattttt gtcagggtta tagcatcatc accattaaaa
     8101 cttaaagaac catggcttaa atttgcaatc ttaagaatat ctgatgaggc agaactatgt
     8161 gctatcacaa aaacatcacc attatttaag ataccattta gattaatatt agcgccagga
     8221 ttttgggctc cattataaaa aatttgtacc ttataatcag ataaattcac tggatgtcca
     8281 gtgccattaa atatctcaag agctttatta ttacttgaac cttcaacata ttcactgata
     8341 aacaaatcct cagctaaagg tgtggaagct ttaagggttt cagaagaatt agttatccct
     8401 ttggtaattt gactatctat ttcagacaaa tgattaccta aacttatctg acatataagt
     8461 agggtcaaca gtaaggtggt aatttgcttg attttcattt aatatccttt tttcctatga
     8521 ataaaaataa ttttatattt cttttttaac tctataatca aaagcatatc aatagattag
     8581 attgagctaa ttgactacat taccatgaga aatttataaa taaaattgac aagtagaaat
     8641 ttaaaacttg acattatgat gggttattgt tatttatatc ttatagggaa taatatttag
     8701 agaggtaaaa atgagtaaaa ttagattaag tgttcttatc ccgcttttat tattggcatt
     8761 tattccagct atgtatgcct ttgaaagcac aatgagttta gaaggaggga ttaattttcc
     8821 ccaggatgat gtggaaggag ataatgtatt gatggggtca tgggcagctt cttgggattt
     8881 gtggtgcttt ggagatctgg gtataggaat aaatccctgg tttacaaatt taaaggtaaa
     8941 aaatggacct aacgattatc attcatcgtt agaaggagtt aatctctatc tcaaatttaa
     9001 gcctaccaaa ggggtagcag aaaatttccc gagggattat actattaata gggtttcccc
     9061 gtttatcgca ttaggtgctg gatggtcaac tcataatagt gaagatgaat atggtaatat
     9121 tgatgatgcc agtccagaca aaattggtgg tcatttaact tttccttatg cttcaattgg
     9181 tatatcttta ctatctaaat ggaacactac tctggattta ggattaaaat atactatgtt
     9241 tgcatcggat aagattgatt tagtcaaaga tggtgatttt aatgatgcac ttttgactcc
     9301 ttatattgcc gttggcatgc atttcggtag aaaagaaccg cttcctaaga agattgatcc
     9361 agagatagtg aaactccgta aggatttgaa tgcaactgat ttgagattaa atgaagttat
     9421 agagcagctt aatgcagtca ctctgcaact tccagaattg caaaagcttc agatgtcaat
     9481 tgagcaatct gaaacaaata ttgctactct gcaaactcag tatgctcaga tgaatatcaa
     9541 ggtaacagag cttacaaaca aagttaacaa tttagaacaa cgcgtagctc aggttcctac
     9601 acaaaaaaca ccctcagctg atatttatgc caatgaaatt aaacaaataa aagatactca
     9661 atctgcttta gcagcaaata tggcaactaa agaagatatc gctaaactgg aacaagagaa
     9721 agctgagatt gctattactg tgaatcagat taataacgaa ttggcagaaa taactaaaaa
     9781 attagaacaa cttgacgctt taactgctca ggttaatgct attaagggtg cagctgatgt
     9841 ttctgcttat accaaagaac ttgaagaatt gcgtaatatg ctcaataact atgaaaagga
     9901 acttgctgcc ataagaacgg ataccgaaaa cttacataag gcagataacg atttggctgc
     9961 taatattaat gatcttcgta gccgtttatt aactttgagc gatgagctta aaaagcttag
    10021 tgaaaaacca aaaccggtag ttactcccga accgcaagtt caagaaaaac ctcaagtgat
    10081 tgaaaaagat tatctcactg aacttgaaga agaactaaag ggtaagatga ttcattttgc
    10141 caccaatgct acaaccctca gtgaaaagga taaactcttc ctggataaag ttgcagaata
    10201 tctgattaaa cttccagaag ttaaaattga agtgcagggt catacggata atactggttc
    10261 cgataagatt aataatacta tttcttataa cagagccaaa agtgtggctg attatttaat
    10321 tgctaaggga gttccggaaa gccagttgat agtcaaaggt tatggtccaa gcaaacccat
    10381 tgcttccaat aaaaccgctc aaggtcgggc tactaatcgt cgtgttcaat tagtaattgt
    10441 caaatagacc tcccataatt aaaacttcgg ggaatctggt ttatcagatt cccctttttt
    10501 atttccattc tgtctttaaa caggtacata atgtcgttct tatctcaaac ttatgtccga
    10561 tttcggacat aagctggaca taagctggac atnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10681 nnnnnnnnnn nntgggcttc ctttgcagat ttatatatta acgatgcttt tggttcctgg
    10741 caagctcatg cttccactta taatatcacc aagtttttgc cttcctatgc aggattattg
    10801 atgataaagg aagttcagaa tctggaaaaa gtttttaatc cacaacgtcc acttctttct
    10861 gtggtcgcag gagcaaagtt tgataccaag atgggtccac tttcagcttt gttaaaatta
    10921 agtgataaat tagtgttggg tggggttcta tacaatggtt atcttgctgc taaatataag
    10981 gttaatataa aaggtttatc tccagaggat ttgcaattag ccgaacaatt cctgcaagaa
    11041 gcagaaaaat tcccggataa aatagtggaa atgcccttca taatagagtg cgatagtatg
    11101 gaagatgtaa gcaattggag aattagaaaa gtgactgatt tgcaagctgg aatggattta
    11161 aactatatat tagacattgc tccagaatcc ttttctgact cctctattca ggaagttttc
    11221 tcttccgcag caacaatttt tgtaaatgcc gtgatgggtt atacagttct gtttaaagaa
    11281 ggcactaaag ctctttatag cttaattcat tcctgcagtc aggcacaaaa gttatttggt
    11341 ggtggtgata ccatccaaga atttggagat ttgttacctg gaatttttgc tcaggcaaaa
    11401 aatgatcccc gctactattt ctttactggt ggtggagcag ttttagatgc catagctcaa
    11461 ggttcacctt atggaatgaa acctgttcaa gctttgttac aaaagataga aaagtgaagg
    11521 aataaaaaaa tggaatggga aaaagaactc aaattactgg aagactttcc tccgccaacc
    11581 tatgaagaat ggagaaaagc agtagaagat acccttaaag gagctgattt tgataaagtg
    11641 atgcatacca gaacctatga gggcatcact ttacaaccga tttatagaag agaagattta
    11701 agcaaactac cctttcttga ctcgcttcca ggtaaagctc catatgtacg aggaaataat
    11761 ccgaaaaagt ttttaagtga aggatggtta attgctcaat cccatagaga gtccgattta
    11821 aaagcactca atgctcagat attaagggag ttgcaactgg gtttaacagc tgttaatata
    11881 cagcttaagc acgatgatga tttaagaggt gtttctatca agtctattga tgatttaaga
    11941 gcaatctgtg ctgctataga cctcagagct gctcctcttt ttatgcaatt ggatatggat
    12001 gattatttta ttctcttgct ggtagaacaa tatatttgtg agagaaaata tgcctgggaa
    12061 gaattgcaat taggagttgg atttgatcct atctcagaat ttgccagaaa aggatattta
    12121 agttttagct gggatgaagt ctggggaaaa atagtagaag gtgtggagat tgcaataaaa
    12181 aatatatcag gtttacgctg tatttctata gatggtagtg tctattctgc tgccggagct
    12241 tcttccactc aggaactggg aattatatta gctacagcaa tctattacct tcagaattta
    12301 ctggataaag gatggactat agaacaactc agtcctctct ttcaggtaaa gctttccctt
    12361 ggacccaatt tctttatgga aattgcaaaa gttagggcaa tgcgtcttct ctggtctgaa
    12421 atgattaaag cttttggtgg gaaagaaaaa gaacaaaaaa tctggattca cggtaaaact
    12481 gctactttca accaaagcct ttacgatgtt tatgtgaatt tacttagaaa tgcgactgaa
    12541 gccttttcgg gtgtaattgg tggagttgat agtttagaag ttggcagatt taatgaatta
    12601 acctcagaag aagatgaatt ttccagacgc ttagctcgca atcagcaaat cattctgaaa
    12661 gaagaatcac attttgataa agttgtagat cccgctggag gaagttatta catagaaaat
    12721 ctaactcaag aactcgctaa tcaagcctgg aaatatatgc aagaactgga agctgaaggt
    12781 gggatactga acgctttgca agaggggaaa attcatactg atatagaaaa ggtagctaat
    12841 gcaagaaaag aagccatgcg gaaaaggaaa gatgtctatg tgggaattaa tctttatgca
    12901 aacccagatg agaatccacc aacccagatt caaattccaa gagagtccaa ccccataaag
    12961 atggaagttt tagaagctgg agctttacca caattgagag tagtggaaga aatagaaaat
    13021 ttacgcactc gggttataaa atctgataag aatacgaata tattcctgat gaatatggga
    13081 actatcaatg attatagagt tagagctgat tttgctacag gattctttca ggcaggaggt
    13141 tttacggtta tttctcctca aggttttatg actgtggaag atgcggtcaa atcagctaaa
    13201 gaatcaaatg cagctgccta ttgtatctgt tccacagatg agaaatacaa tgaacttgtg
    13261 ccagcgatat gttctgctct tcccgatagt tttctaattc tggcaggtta tcctaaagat
    13321 aaagtagaaa gttttaaaga gaatggaata aagatgttta tttatatggg tgcagatgta
    13381 gttgccacct tagatgaatt agctaaaaaa ctgggggtgg aaaatgaagc ctgatttcac
    13441 gaaaattaat ccagaattta aagtagaagc taaaagcaaa cccgaagaat tacctatctg
    13501 gaaaactaat gaacaaatag aagttaaagg actcttcaca aaggatgaca tagctcaagc
    13561 tcagcatctt gattatcttt ccgggttacc tcctttctta agaggtccct atccctcaat
    13621 gtatattttg cgtccctgga ctattcgtca atatgcaggt ttttccaccg ctgaggaaag
    13681 caatgctttt tatagaagaa atctggcaat gggacagaaa ggtctctcta tagcttttga
    13741 tttacccact catagaggct atgattcaga ccatccacga gttgcaggtg atgtgggaaa
    13801 agctggagta gctgtagata gtgttttgga tatgaaaatc ctctttgaag ggattcctct
    13861 ggaccagatt agtgtttcaa tgactatgaa tggtgcagtt attcctataa tggcttttta
    13921 tatagtggca gcagaagaac aaggagttaa acctgagcaa ttaaatggta caatccagaa
    13981 tgatattctc aaagaatata tggtcagaaa cacctatatt tatcctccag aagcctcaat
    14041 gcgtattatt gcagatattt tccgttatac cgcagaacat atgcccaagt ttaattctat
    14101 cagtatatcc ggttatcata tgcatgaagc aggtgccact gcagacctgg aaatggctta
    14161 tactctggct gatggcttgg aatatgtgcg cacagcactt aaagcaggat tggatataga
    14221 tgttatagga cccaggcttt ccttcttctg ggcggaaggt atgaattact ttatggaagt
    14281 ggcaaaattg agagctgcca gagttctctg ggcaaaaatt attaaacaat tcctacccca
    14341 aaatcctaag agtatggcac tacgcactca ttcccaaact tcaggctgga gtcttactgc
    14401 tcaagaccca tttaataatg taactcgtac ctgtatagaa gctttagctg ctactatggg
    14461 tcacacccaa tccttgcata ccaactctct ggatgaagct attgccttac caactgattt
    14521 tagtgctcgc attgccagaa acactcaact ctatctgatg aatgaaactg gaattactcg
    14581 ggtgattgat ccctgggcag gttcttatta tgttgaatat ttaactgatg ctctcctgaa
    14641 gaaatcctgg gaacatattc tggaggttga agaacttggt ggaatggcaa aagcgattga
    14701 aaccggtcta cccaagatga gaattgaaga agcagctgca cgaagacagg ctcatattga
    14761 ttctggagaa gaaaccatcg ttggagttaa taaatattgt cccgaaaatg aagagcctat
    14821 agaaattctg gaagtagata atacagctgt tcaggaagcg cagattgcgc gtttgaaaaa
    14881 actcaaagca gaacgggatt ctcaaaaagt tcaggcttgt ctggatgcca ttacagaagc
    14941 agcaaaaaat aatacaggta atcttctgga actggcaatt gaagctgctc gcgctcgtgc
    15001 taccttaggg gaaatttctt atgcaatgga aaaagttttt ggcaggcatc aagccaccat
    15061 tcaattgata aataatgttt atagcagtga gtatacaaaa atggatgaaa tagaaaaagt
    15121 aagagcttta accgataaat ttgcggaaaa agaaggaaga agaccacgca ttttagtggc
    15181 taaattaggt caggatggac acgatcgtgg agctaaagta gtttctactg cttttgctga
    15241 tatgggattt gatgtggatg tgggaccact gtttcagact cctgctgaag ctgcccgtca
    15301 agcagttgaa aacgatgtgc atattatagg tgtgagttct ctggcaggaa gtcacaaaac
    15361 cctacttcct caattagtgg aagaactggc aaaattggga cgagatgaca taatgatagt
    15421 ggttggaggt gttatacctc ctcaggatta cgattttctc ctggcaaatg gggctacagc
    15481 tatcttcggt cctgggacaa aacttcccga agctgctact atcataatga ataagctctt
    15541 acaagaataa aatgacaaaa gaaagaaaac ctgagtggac tccacccaat gcaggaaagg
    15601 aatatgcttc tcgtgtcctg aaagcaaaag caaatcaaag tgggatagtc aaagaaacgg
    15661 gcaaaaagag aagttctttc ccttctgtgg atgaactggt tgaaggtata aagaaaggtg
    15721 atagaacact tctcgctaaa gctattactc tcatagaaag taatgctcct cagcattttg
    15781 aagccgggca ggaacttatt agaaggcttc tgcccgattc tggtaattcc ataagaattg
    15841 gggttacagg aatgccaggt gctgggaaaa gtactttcat agaaaatttt ggtctatggt
    15901 tactcggaaa agggcatagg gttgcagttc tggcaataga ccccagtagc actatttctc
    15961 gtggtagtat tctgggtgat aaaaccagga tggaagaatt aagcaggcat cctgatagct
    16021 ttattcgtcc ttctcccagt ggaggcactt taggtggtgt cgctcgtaaa accagagaaa
    16081 ctatcatact ttgcgaagct gcaggttttg atattgtgct gattgaaact gttggtgtag
    16141 gtcaaagtga agtaaccgtt cgttcaatgg tggatttctt ccttttaatg caaattccag
    16201 gtgcaggtga tgaacttcaa ggaataaaaa aaggggtgat ggaactggca gatttagtgg
    16261 tcgtaaataa ggctgatgga gagaatattg cagctgcaga gctggcatgc agggaactta
    16321 ataatgctct ccattatctt cgtcctgcca ctgaaggttg gactaccaaa gctataacct
    16381 gctcggctct ccagaagaaa ggactttcgg aaatctggga aatcatagaa aagtttgtag
    16441 aacaaggcaa aagcagtgga atattctatg aacgacgcaa aaagcaggtt ttagaatggt
    16501 ttgaatcgct aattaaagaa gcggtattaa atagatttta tagcgaccat aaagttcagg
    16561 aaaaacttcc tcttcttaag aaacaggtag aagaaggttc acttcctgtt atgcttgcgg
    16621 ttcagcagtt actaaatgtt tatgaagatt aactggagta atagacctgt ctgattgcct
    16681 catataaaat tattgctacg gaattggcaa gattaataga acgaataagt ggatgcatcg
    16741 ggatagtaat taagcgttca ggatacttat ccagcaagtt ttgaggcaaa ccacgagatt
    16801 cgggtccaaa aacaaaggta tctccatact gataatgctc atttagataa tttcgtttgc
    16861 tttttgtggt gcagaaaaat actcgctcta aagggcaaaa ctctaaaatc tcttcccaag
    16921 ttttatgcat agtcaaatcc agatgttccc agtaatctaa tcctgcacgt tttagggtct
    16981 tatcagtgat taagaattta cagggttgaa ttatatgtag tttagaacca gtcccaacac
    17041 atagccttcc gatatttccg gtattggcag gaatttcagg ttcatagagg acaatattaa
    17101 atcttgtttc aaccaaagga atcatatcta aaaccttata tttccataca tcatccgctt
    17161 ccagttagac atccagacct gaaggctatc ctgaactggc tgttgttcca atctattgtt
    17221 atgtgaatag attatagctg gtatatgagc aaaagaagta ggaagtggag ttctaccatt
    17281 aaccggcatc atagctaatt tatacattac aaattgttga ggatttggtg atatgagata
    17341 ttttaagaat tgcaaagcag tgtccttatg gggagcatcc ttacataaag cagctgactc
    17401 tacatatagg aaacttcctt cttctggaat gattgacttg atatgacttt ctgagggata
    17461 aagttcattt agccaagcag gaatagtgtt atatccaaac attaaaccac atttaccggt
    17521 cctgatagca tcaaggcata aggttggatt ggggtatatc ttataaatat tctttcgtag
    17581 agaagaccac aactgttcat atccttgctc agaaaataat gccacagtcc agagaagagt
    17641 gctccttcct aagccacatt gagatgggtc acagatagct aattgtgaat aatatttact
    17701 atcctgaagt tcaccaaaag aatgaggtgg ttcaggatat tttctggaat cataaataaa
    17761 agccaggttt gcactcgcat agggaattag acaatgctca gaatcccgag gtatttcata
    17821 tctaatctct tgtaatgata cttccggaac gggagcaaaa taatcgctgg aagcaatatc
    17881 gtaaaaaaat gaattatcca atcccataac tatatccact tttccttggt aatcaggatt
    17941 ggataaggtc tgcattaaca tagaaagatc ggggaaaagg tttattctca gtccgatatt
    18001 attctgttta gcaaaatcct tgataacaac ggtttcaaaa cctgattctc taatgtggtc
    18061 cagagcaaaa atatataagt aggagctcgg ggttatttgt ttttttactt ccttattgtt
    18121 ttttttgcag ctgaatcctg atatcagaca gagcactaag gaaatgatta taaataattt
    18181 tacagacctt tttctaagtg tcatagatag tgtgccatca tcaaaatagc caccagaaaa
    18241 tcaagtacta aaactccaac tgaactgtaa acaacagttt gcatagtgct atgtcccact
    18301 ccttcagcac caccggtggt cttatctcca aaaaaacaag ctaaagaagt aatcagaaat
    18361 ccgaataata tagcttttat gattccgcca attaaatcaa agggggcaaa atagatgcgc
    18421 atatcattaa aaaagttaaa gaatctgata ccatatctca tccaggtgaa aaaccaaccc
    18481 gcaattatac taattaaatc cgcataaatt gttaatagtg gagcagaaat aacaccagca
    18541 ctaatacgag gaagataaag aaattcttct ggagcaatac tcatactttg aagggcatca
    18601 atctgctcgc tcactctcat agtccctatt tctgccgcta tggaagcgcc aatttttcct
    18661 gttaaaacca gacctgtaag taccggtgcc aattctatca tatttgcttt tccaatgata
    18721 acactgaata aatttttagg tatataatag ctaccttgat aaataatttg cactgcgaca
    18781 actaaaccag tgaatataga agtgatggta attaaaaaga gagaatcaac tccgatgcgt
    18841 ttaagctgaa tggttagttc ttgccagcgc ttaggaagtt taggaaggcg ttttaggact
    18901 cttccaataa atatagtata agcaccagta ccagcaagaa gcttaaaatt catgaatggt
    18961 atctaagtta cgaaacatgg taaattcttt attaaatcct agttcaacag taccagtaga
    19021 gccatgacga tttttgccaa taataatttc agcaatgcca ggtttttccg atttttcttt
    19081 ggtgtaatat tcgtcacgat aaataaacat taccaagtct gcatcctgtt ctatagcacc
    19141 agattctcgt aaatcagcca gtcgtggacg tttatcatcc cgattttcca gaagccgatt
    19201 taattgagaa agcgcaatga caggtatttt catatcctta gccagaattt taagtgcacg
    19261 agatatctca gcaatctctt gttggcgatt atccttatct cgtggtaatg tcataagttg
    19321 caaataatct atgataatga ggtctaaacc gccaatttcc gaagctaaac gcctcgtttt
    19381 ggctctaatt tccaaaggag tattggttcc ggattcatca atatatattt gtttagtgga
    19441 aatcacatcc gccacctgca taatccggat tagcttttcc tcattcatac catagccttt
    19501 gaggattgat tccatactga cttcagcggc actactgaac atcctcatta ccacttcatc
    19561 tgcagccatt tccatagtaa agatagccac tttcttaccc aaatctacgg cagcatgtga
    19621 agctatattt agcgccagtg aggtctttcc catagcagga cgtgcagcta taataataaa
    19681 ttgccctggt ctaaatcctc ctgccagacg gtctaaatct ataaatccac tggaaacacc
    19741 gatgacagga actttggtgg tggaaatagt atcaataact tgaatgactt cagcactaat
    19801 ctggtcaatt ctttgaaacc cctgatgttg tggcatttca gcaattttaa aaatagcttg
    19861 ttcagcttca tcaacaatct tttttactgg ttcagtagag ctgtagcaag cttcaataat
    19921 gctgttacag gtaatgatga ggttgcgcaa taaagctttt tctatcagga tattgagatg
    19981 ataattgaaa ttggcactgg aagtaacgaa atccgaaagt tcatttatat aaggaatacc
    20041 acccactttt tccagaaaac ccttttgttg tagacggtct acaagtgtta gagggtcaat
    20101 ttccactcca tcactgaaaa gttcagctat agtccgaaag attaatttat gggaagtgcg
    20161 agaaaagtat tcttctttaa ttaattccag acctttactg acaatgctgg aatcaattat
    20221 catagctgat aatacacccg cttcagcatt tacatcagct ggaagagccc ggtctgaaat
    20281 ctgggttatc tctttgggaa tagtttcctt ttttgccata tcattccttt ttaaatttat
    20341 ccctgagagc tttagctacg actggtggaa caaagtcttt caattcactg cccaattcag
    20401 ctaattgtct ggtaatggaa gaagaaagat acatataacg aagactgggg accagaaata
    20461 cggtttcaat ctctgggttg agcgtggtat tagttaaagc taaggaaagt tcatattcaa
    20521 aatcggaaac agctcgtaat cccctaatca ttaccgaaca attctgggac cgagcaaaat
    20581 ctacagctaa tccttcaaag gtatacactt ctacattgga aagatgttta actgattcca
    20641 ggcatagttc atatcgttcc tgcagactga acaaagtttc tttatcggta taagctgcca
    20701 cactcaatat aactttatca aaaagtctgg atgctttttc cagaatgttt atatgtccat
    20761 aagttatagg gtcaaaagtc cctggataaa tagcgttagt gctcattttg aaaagcctct
    20821 tgtctgatgg attctatttc cgatatttct ttaggtatac ctgcagaaag cacttcatga
    20881 ccatcatctg ttaccaatac atcgtcttca attcgtaccc cgagcctttc ttcaggaata
    20941 taaatgcccg gttcaaccgt gataacattt ccaggggtaa ggactgcttc tctaccaccc
    21001 acatcatggg tatcaagtcc aagaaaatga ctaacactgt gcatataata ctttcttact
    21061 tcatccttgg aatttattaa gccaatggaa agcattcctt cagccagcca ttccaccgtc
    21121 ttattattca gaacggatag ctccactcct ggttttacca attcaattat ccttttatta
    21181 acatccagca ctatattgta aatctcagcc tgacgataac tgaatctttg ctcaataggg
    21241 aaacatcttg taatatctgc actataaccg ttgcaggatg ctcccacatc cattagtatc
    21301 aggtcgcctg gttgaatctg gcaattattc ttttcataat gtaaggttgc cgcatttatc
    21361 ccggtggcaa ttataggtgc aaatccccat tgctgtaatc cattaacttg cattctatag
    21421 aagagagtag cttccaattc atattccatc attcccgctt ttgcagtatg cagaatatcc
    21481 ataataccca aaccggttat atctatggct ctcttaagtt gctctatttc ccattcatct
    21541 ttaaccagtc gtagtggagt aatcaaatca cccaagtccc tgatttctat atgaggatat
    21601 ttttctctaa caggttggag cataaagaga gggtaagttg caggtttatg gagcattgga
    21661 taacccagat tggaatagat tctgcttaac tgagaacaca ttccagaaag atagggataa
    21721 aactcgtctt tatagcgtat ttgctggatt ccacttattt cttttgcctc ctccatagat
    21781 aactttgctc cttcccacac caccctttct gggtttcctc tttctataaa gagcacctca
    21841 ttccatctac ctgacacctt tgctgctata tagataagtt ctggctcgtt aattccagta
    21901 agataaagaa aatttgaatc ttgcgagaat ttttccagag taatctccgg tttagcaaag
    21961 actataaccg attcattatc tcctaattgt tcagacagtt tttcccgcct ttttttagct
    22021 atatcagcgt ccataacact tccttaataa taaatagatg tagctctata tttttcctca
    22081 gcttaaaatc agcaaaagga tatttattta agttcggggt aattctgttg tttgatgctt
    22141 atcgggttat ggcagctctc ttacttgaac ttttactgga aaatacaaat tctggctctg
    22201 ctcctttaga tactacatca gcagtaatat ggcagcttcg gatattgtcc agatcaggaa
    22261 tgttatacat aattttgagc ataaattttt ccataataga gcgtaatccg cgagcaccag
    22321 ttttttgatt tattgctata cgagctatct ctcttaatgc atcttctcca aattcaagtt
    22381 ccacaccatc cagttcaaag tatttcttat attgactgca cagagcattt ttaggtttta
    22441 caagaatttc caccagggct tcttcagtca attcatgaag agtgcaaagc actggcattc
    22501 tacctatcaa ttcagggatt aaaccatatt ttaataggtc ttgcgagata gtctggtcaa
    22561 agatattagc ttcgtcaata tttgtaccta atttaacatc aaatccaatg gcttttttat
    22621 ctattctttc tttaatgatt ttttccagtc caaaaaaggc gccaccggct atgaataaga
    22681 tatttttggt gtccatttca ataaaatcct gctgaggatg tttccttcca cctttgggtg
    22741 gcacatttac tttggttcct tccaggattt ttaatagagc ctgttgcact ccttcaccag
    22801 aaacatcacg ggttatagaa ggagtctccg atttacgact aatcttatca atctcatcaa
    22861 tatatacaat tcccttttct gccttagcaa catcataatc tgcagcctgt aataatcgga
    22921 ctaatatgtt ctctacatct tctcccacat atccagcttc cgttaaggtg gtagcatcag
    22981 aaatagcaaa aggaactttc agaaatcttg ccagagtctc agcaatcaga gtctttccac
    23041 atcctgtggg tccaatcatc aaaatgttgc ttttctccaa atcaatatca tcatccttcc
    23101 ggctaaagat gcgtttataa tgattgtata cagccactga aataatcatt ttggcttctt
    23161 cttgacctat tacatactgg tctaaatatg cctttatttc actcggtttt ggtaaatcta
    23221 taatctcttc ttctggttcg ggtgcattag aaaccattat attatgacac attgttatgc
    23281 attcgtcaca gatatttcca gctatgcccc gaattaaggt tttaacttct gtctcattcc
    23341 taccacagaa tgagcaggtt agatttttat atctctccaa caattcctcc tacaaaaatg
    23401 tttcgtatct ttttatcata gataatataa aggagttttg aaatttcgtc aagttatttc
    23461 cgatggtcaa taacttcatc aattatgcca taattcttag cttccatggc atccatataa
    23521 tagttacgtt ctgtatcctc tctaattttc ttgatgtcct gacccgtatg agaatgcaga
    23581 atttgattca ttctttcacg caaataaaga atttctttag cctgaatttc tatatcactg
    23641 gcttgacctt caaaaccacc taaaggttga tgaatcatta ttctactatt gggtagagca
    23701 gtccttttgc caggactacc agctgctaaa aggactgcag ccatacttgc agcttgacca
    23761 atgcagatag tgctcacttt aggttttata tattgcatag tatcataaat agcaagtccg
    23821 gaagagataa tacccccggg actatttata tacatataaa tatcgcgttc tgggtcttca
    23881 ccttccagat gtagtaattg agcaacgaca gaatttgcca gattatcatc tattataccg
    23941 cttacaaaca caatgcgatc ttttaataag cgtgaatata tatcataagc tcgttctgtg
    24001 cgtcctactt gttctataac aatagggata taactcattg ttcttccttc ctcacatttt
    24061 cctccgaggg ttctacttgt tcttctggtt tatcttgttc ttcctcttct tctggttcat
    24121 acggggcaat ctcataagca ggatattctg tctctccagt aggttctata aaagtgcagg
    24181 tctcagctat tttccttaag agaaagtaat cttgagcatc tctaataaaa ttgtcgcttt
    24241 taatatagtc ttcatttttt tctttataac cggcaacgga tgaattggag agaattgccc
    24301 tatgttctat aaactcatta atcatatcct ctgaaacttc aatatggatt ttttctctta
    24361 aagaatccac aattaaatac cgcactaaat aattggctgc ttcattaaca taataattat
    24421 aaagaggatg attttcgtca atatgtagag aggcagcata atcgtcaatt attgatttaa
    24481 gcgtcattgg tggtatttta aagggttttt tcttgaacag cgcattgata attgcattat
    24541 tttgagcttc ataattggag tgttcaacct gagcttttaa ttcttctcct attttagctt
    24601 tcatttcttc aagattctca aaatccatat ctttggcaaa ctcatcatct atctcaggta
    24661 taataagttt ggttatagca tcaatctcta tggttacatc catttgagtt tcatcttcaa
    24721 aatcaattgt atcatcatct atgaattgtt ttaaggtctc aatgggaaga gaaagtgata
    24781 ccttatctcc tattttttta ctgataagct cttttaatgc aggtagattt tctaattctt
    24841 cagaataaat atttacgcta taattatagt cttcaccctt ccaagtaaaa tataatctac
    24901 actgtagggt cgtgttttct tcaactgtat ctacatcttg aattgtgcgt tgtttccatc
    24961 ttaagtcact taaaaattgg tttactgcat cttctaaagt ttgaggttta aaggggactt
    25021 ctaaattttc taacagatcc aaagttagat ccggtatatg ttctaagatg acagttaact
    25081 tcatatccga tccctggttc caattaagag attttaattg aggagttgcc agaaaattaa
    25141 gattattttc tctaagaacc tgatataaag agtctatagc aaatttaatt tcaaaatcct
    25201 cttttatatc cgcgccatga agtttttcta tcatattttt cggagcttta cccttcctga
    25261 aaccaggtat ttccagctcc tgagctttct tattgaaata tttcttataa gcttcatcta
    25321 ctttttctgc ttcaataact acagtaactt cactacacac ttcattaatt ggtttaatct
    25381 ctgtttgcac tttttctcct aataaatttg gtgcgaaaga ggggacttga acccctacag
    25441 gttaacccta ctggatccta agtccagcgc gtcttcctat tccgccactt tcgcttttta
    25501 ttcaattttt tataatgctt tgcaatgtca aggaaggagt tctatgttat ctatcaacct
    25561 tgtatttcca atgtatactg ctaacatcat ccttgaacca gatcggacct ctggcaattc
    25621 ttgtagtgtg gaaccgtcca caatttttat ataatcaatc ctaccatctg attgcttaat
    25681 gatattttcc gcttcggaaa ttaaaatact ggaatctaaa tatccctcat tgaccatttg
    25741 ttgtgcattt tttaaagctt ttatcagaca ggtagccctc tgtcgttcat ctggagtcaa
    25801 gtatgaattt cgtgaactca tagccagtcc atctggttct cttactatag gacatcgcac
    25861 aatctttgtt tctaagttca aatcctctat catcttttct aatacaatta tctgttggta
    25921 atccttcatt cccataaaca tcaatttagg atttacgata tgcatcaatt ttaaaacaat
    25981 agtggcaaca cctctaaaat gtcctggacg acttgctcca caaagaatat cggacaatcc
    26041 ttccacctga acccaggtgc aatatccttc tggatacatc tgaacatcat ctggcgtgaa
    26101 cactatgtcc acatgataat ttcttaataa atctatatct ttttctaaat tgcggggata
    26161 atgattcaaa tcttcctgcg gtccaaattg ggtgggattt acataaattg aaactaccgt
    26221 gagatctgat tctgaatgac atctttctac cagagataga tgacctttat gaagagctcc
    26281 catggtgggc acaaagccta ttttcttttg taaagccttc ccggctaatg cttctctgat
    26341 ttcggctatg gtcttatata tctgcattat ggtaatggat tgttatcttc gtccaataga
    26401 atgatacagg gttgataact atctaactct tcttcatcca ttagcccata atttactata
    26461 attactatat ctcctacttg cacttttctg gctgctgctc catatatacc tatagtcctg
    26521 cttcctggtt ctccaggtat gatgtaagta gaaaaccttt caccattgtt aatgtcccaa
    26581 acttctacct gctcatattc ccgcattcca gaaagcttga gcaactcctg atctatcttg
    26641 atactggcat tataatgcat attacattct gtgactgttg cccgatgaag cttggacagt
    26701 aacatttttc ttaacatcgt ttcacctcta aagaataaat tactaatcca aaaaatagca
    26761 cttacctcca atgtcaatta aattccctct aactttaaat tatgactcct atggaaatag
    26821 aaatatcatt cccgaaatta caaaactatc attattatta aaggaaatct ctctaagagg
    26881 ggtattaaag tatgaagaaa gtaggacgga agagataaaa aatctgattt actatttttt
    26941 cttctttttt taagagattg gaattatata tattatatag agttagaaaa gggataagaa
    27001 aaaaaggaga aaaaattaag gaatttttta atattttgta ttgacagaat aaagattata
    27061 tttttttagg ttcaagctgt ttttataatt caacaagtga tagttctttt tcaaaaatga
    27121 tagtgtgcta tagagatttg tgcgagccaa tttaagtcaa ttaacttcat attttattat
    27181 ggagagtttg atcctggctc aggacgaacg ctggcggcgt ggattag
//
LOCUS       DAORZF010000019        27096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_1521_length_27096_cov_365.136718, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000019 DAORZF010000000
VERSION     DAORZF010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 27096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27096
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1521_length_27096_cov_365.136718"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PK015_03505"
     CDS             <1..195
                     /locus_tag="PK015_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33569.1"
                     /translation="GWNYVILPKTGDSLLTCFSASVVLQLLALKMSVRKMKKLDKLNV
                     KDHGVHPDVPKNVSKSITVD"
     gene            201..674
                     /gene="ispF"
                     /locus_tag="PK015_03510"
     CDS             201..674
                     /gene="ispF"
                     /locus_tag="PK015_03510"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOL33570.1"
                     /translation="MLRIGYGYDVHRLVAGRKLILGGVEIPFDKGLEGHSDADVVLHS
                     VIDGLLGALSLGDIGSHFPDTDPQYEGIDSRILLQRTFSLVQAEGYVLNNLDCTICAE
                     SPKLQPYLDKMRKNLAKDLACDISQISLKATTEEGLGVSGNGGISATCILLLRKQ"
     gene            671..1429
                     /gene="mobB"
                     /locus_tag="PK015_03515"
     CDS             671..1429
                     /gene="mobB"
                     /locus_tag="PK015_03515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HOL33571.1"
                     /translation="MKAIGVFGYHHTGKTTLASMLISALKERGYEVAAIKDIHNENYH
                     SDKEGSNSWRLAEAGANPVFAKGLYDAALIFHPAPELPDMLHFLNADWLVIEGFQEAA
                     LPKILCAENLGQIKELWNPTVIAISGEISQTLQNYQDTPVIDYKTDFNRILNLVLEKA
                     FDILPQSDPECCSACGKTCYQMTIDILQGKAKREDCVLDNHNSISISIGGKPLTIVPF
                     VQNLFRDTIIAMISNLKGVNPSQDIEIRIKGKDD"
     gene            1422..2039
                     /gene="thiF"
                     /locus_tag="PK015_03520"
     CDS             1422..2039
                     /gene="thiF"
                     /locus_tag="PK015_03520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS adenylyltransferase
                     ThiF"
                     /protein_id="HOL33572.1"
                     /translation="MTELFNELFANQNPEYVKIWQNSVVGIAGVGGLGSNIAFSLTRA
                     GIGKLIIADPDIVSTSNLTRQQYFLNQVGLPKVAALKDNLLNISPFTMVNPYPQKITP
                     TNLSLIFGKVDILIEALDEAEEKQMLIENWQQLYPDKYLIGASGIAGVGKNELIHIEQ
                     IGTLFLVGDGITEIENGVYPTAARVAVVANMQANLCLELLLQKGN"
     gene            2042..2245
                     /gene="thiS"
                     /locus_tag="PK015_03525"
     CDS             2042..2245
                     /gene="thiS"
                     /locus_tag="PK015_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HOL33573.1"
                     /translation="MPTITVNGQEIPWEEGMTIRTVLNKMNYSFRMLVIKVNDKLVKK
                     EDWDSFQVPPGADVKVIHLISGG"
     gene            2276..>2940
                     /locus_tag="PK015_03530"
     CDS             2276..>2940
                     /locus_tag="PK015_03530"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33574.1"
                     /translation="MPKTLSLLEVYNILLNFQGPQHWWPGETQDEIIIGAILTQNVSW
                     KNVVKALNNLHSAGIYTLKDLDALSVEAIAPLIRSTLYYNQKAIKLKTFTNFLRERYQ
                     FNLQQMFAEDLKTLRSEMLKLKGLGPETVDSILLYAGSKPVFVVDAYTIRLLTRLGFT
                     DKKMNYSQWQEFITERIPQDVELYNDFHAQIVNLCKDICRNKPRCEICPLTLYCTYYQ
                     QNK"
     assembly_gap    2941..3040
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3471..6377
                     /locus_tag="PK015_03535"
     CDS             3471..6377
                     /locus_tag="PK015_03535"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOL33575.1"
                     /translation="MGSNNYTLLNVPFSSNLIDYALTQVSDRCIFVFPSRISAEKARE
                     KFLPNWKFEACEFISIEDFKDWLILPSEPPLTDEKRLLCLYQALTLEDKKFFKFYTYF
                     DLISWGNNFFQFFEELNDEDILPEEIENRIDLREWQQDFLAKILNIRENYQKLLEQKG
                     FTDPIFYRKASQLKIPFSGYKVIWVNQFYYSKLELAILKALAESENEVVVISQTENAD
                     FNPQNRKNTSLNLENLAKNDYLTQKIKVIETENEDQMVISFLGKLGKAKNIEGTTAII
                     DSHYWDKHYRNLMNPALFDFSNSINLINSSPYQFLNILKEHLQALTTLSFEGKEITAL
                     PIKLMLDAVGNESFVHYYFPKDTIQSKEELLNELTTLRKKDVYWLDKNMNCLQVFHTS
                     PHFNKLTSLVENHFSLLEEIAKINSPADLINLIDTEEMSIGLNIKRMCSENEILHSDL
                     LEQFYQRIANFASTDNLQLVQDWNSFFASSGKDLALNVFQLFLDYLENAGYKFFYEID
                     KENNPITITNTMDSRNLRYDNLVIFHAIEGEFPSAPKPVWLLNEAQKGQLGLKTYNDL
                     REWERYYFFRLILTSRNVLIYTYRNSELDIEPGSFVTELQHLNENHKLNELEFDWVSS
                     EVPLSKYYNARQQKYSSGENNLLASYPDYLLNREENPQIDEKDFFILPCEPTKDFGEN
                     LELTCSYYNLQQLLDNTFVWYIQWIQQLQEIDFRPKDTVSAKIVGSLVHRFISDLLSP
                     FNGKPCKIEELQELFYQPVTTLAEYFRDLFTSKDFFFQLPQNYSLKFLKDIYTNYLPL
                     SMQSFYEDFLSTNLADKVFTLFAESGIYDDNLTQEHLLPLPPDNQESEIKVILRGRSD
                     LRIETDDKKFIVDFKTGRNPDKGQLLFYEWLYYRWQQEDMDISPIFWIINDNKIIEKT
                     KYDDANKWRNTIYETLHKCRAEGYKLPKTVTQLKQLKRITRADLYRPKQGGNNE"
     gene            6370..9759
                     /locus_tag="PK015_03540"
     CDS             6370..9759
                     /locus_tag="PK015_03540"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOL33576.1"
                     /translation="MNNSTIKIIKASAGTGKTYRLAVEYVKLVLQYGTNINLDSILVL
                     TFTRKATAEIRERIVEHLELLNSTKDKEKEAKLALIKAIFGNENKTELHDIEKKNLQE
                     ALQKIKTDHQQLQVMTIDSYINNIFRNLVNPQKNYGDFEIDDDIVEKTLPLLYQYLLS
                     SNLELYQKVENLLKARVTSSIDRYNLFFQSLIQNRLMLHLINVSQNHFDPDSLMYLCE
                     HDEICEEKAKESIEKAKKELINYILDIMIIAAGKNFSNIFIVDFQRLFKEVPTTFDEL
                     ITKIEEMLNPTDDAIQQQIRDEQSYKLLKVCIKKLKDNKNIYNAKTLTSKSLGNKTIE
                     WNDYLNEVTEHLSDYFLFKKFIPEQRYILSIWKEVLEEYDKLLYSNKRLTYNDVSWLT
                     LNTLLKGDVESIDWDNSEIDNEFYFFLTHRTRYLFIDEFQDTSLLQFFILKPIMLELS
                     SGEGSEEYGKVVVVGDEKQSIFSWRGGERDLLLNLKDILPTIDKSSGVEETLEESYRS
                     SKAMMDFINYVFSNEALIQDLKLKNLDWEYNECKAAKPQCYPTNIELSLQRYSSTEED
                     FKLDKVQREFIQNFIKPAWEQNKDNKDYTMAILCRTNPQLETFQLLLEEAGIESIYQP
                     SSLITEHNYVYPLISLLKFINYKDWLEFLTVLRSNYIMLKAEPLKKVITIISQSLKDF
                     TEPDFSEFPVVQTLYEFHRKDRDKISDICQDFLDLFLYNKKLSERDYININAFMAILQ
                     DYELNNTDKAKSISAFLDFLDDNKAQDFMKQVPLTGDIPLQLLTIHKAKGLEFDQVFV
                     FYDLSASHNPESYNLSLYPKYKDKTFEKLSDIGITCHYDDILKYSSKKELVELDSKKE
                     MLEEMNTLYVAFTRAKTSLSLCMAYEHKKGFDDLLNNKQESDKYLPILLTKIVRDFFT
                     SKNIEPTGPDENIYKFPVAAETTPKPKEEKESNKERLCALDLTTVLPPKQPYPFKETV
                     ETSKNKYLNWKSMWLENRSNLYGNLAHYYLSFLKYNLPEEHNYALKQCILKYGTLLTH
                     SEIEEKINSLKNNLPISELFPPDYDKVFTEFTLFSEGNQYRIDRLLIDTKQKRALILD
                     YKTGVTKEQEQLEHYKNALSNLPAIKDDNFSIDTKFISLTL"
     gene            complement(10067..10690)
                     /locus_tag="PK015_03545"
     CDS             complement(10067..10690)
                     /locus_tag="PK015_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOL33577.1"
                     /translation="MSLNICEIFYSLQGESTYYGLPCIFIRLSGCNLRCSWCDTTYAW
                     SNGKEMSIEQILQEIRKYPCSLVEITGGEPLLQKRTIELMEILHQEKYRILLETNGTQ
                     SLQNVPDYVVKIVDVKCPGSGFGESFLMENLNFLLPVDEIKFVLASHEDYMFALNFIR
                     NNALEDRILLFSPVIGRLAPETLAQWMLKDGVPAHLQLQLHKLLQLA"
     gene            complement(10728..11921)
                     /locus_tag="PK015_03550"
     CDS             complement(10728..11921)
                     /locus_tag="PK015_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HOL33578.1"
                     /translation="MIEIIDQKCIGCGLCTRACAYGAITIQAKLAVIDEDKCTLCGAC
                     VETCPFEAILIRKQAAEQIDKEQYKGVWVFAEQRDGVIAPVVFELLGKGKELAEQMNS
                     ALTAVLLGSNLENLYPELFAFGADQVIAVDNPALEHFLDLNYARALTELARKHKPAII
                     LAGATVTGRSFIPRVAVQLHTGLTADCTGLEIDPETGNLLQTRPAFGGNIMATIITPN
                     HRPQMATVRHKVMDALPRDDSRKGEIIKEQIDFSDLEDKSFWLCFEKEKTTLVNITEA
                     NIIVSGGRGLKEAKNFALLEELAEALGGAVGASRAAVDAEWIPYSHQVGQTGKTVKPK
                     IYIAVGISGAIQHLAGMSSSDYIIAINKDPDAPIFKVADLGIVGDLFEIVPLITKKVK
                     EIRQK"
     gene            12142..13461
                     /gene="rsxC"
                     /locus_tag="PK015_03555"
     CDS             12142..13461
                     /gene="rsxC"
                     /locus_tag="PK015_03555"
                     /inference="COORDINATES: protein motif:HMM:NF003454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOL33579.1"
                     /translation="MKLKTFPGGVHPPDNKQYTSAVPIEEFPLPQKVVIPLSQHVGAP
                     SVPLVKVGEEVKTGQKIAEANAYVSIPQHASISGKVTKIDTFLHPLGAMSLAIEITGD
                     GSDSWIELVDEPDFMNLPVEEMKNRIKEAGICGMGGAGFPTIVKLSPPENKPIDTVIL
                     NGVECEPYLTADHRLMLEQSEGIINGLKIIMKILNAQHGMIGIESNKPDAIALMNKLL
                     ASEKNLSVVPLKLKYPQGAEKQLIYAATKRIVPAGGLPLDVGIVVQNVGTAFAIYEAV
                     RFKKPLVERIITVTGSVVNNPKNLKARIGTLFSDLVTYCGGTKSEIGKVISGGPMMGY
                     ALPSLDAPMAKGTSGLILFSEKEARTIEERNCIHCAHCVDVCPMNLVPSLIAQGVKGG
                     DLTLAVKAGLEDCIKCGSCAYVCPAHIRIVQWVDTGKLRYAEERQAK"
     gene            13477..14607
                     /locus_tag="PK015_03560"
     CDS             13477..14607
                     /locus_tag="PK015_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOL33580.1"
                     /translation="MKDKFIVSPAPHIHDSTSIPKVMWNVVIALLPALAFSIYYWGWK
                     TLFLTALGVLAAILTEALIQKLRKVPITVYDGSAVVTGMLLTYNISPGSPWWLPVIGS
                     IFAIAIGKQVFGGLGNNAVNPALLGRAFLLASWPSLMTGNCWKPTMAHNLSASSINGI
                     SNLSAISDSLATVSPKAYNLVTGATPLKVMQTLRDTTFINTLTSDPATNIDLGTKIFN
                     GLIDMGTLKSMFWGNIGGCIGEVSVFALLIGAIYLLWKNIIEWRIPFFYLLTVAVLTF
                     LFGGIPGLPETQYSLSLPIFHLFAGGLILGAFFMATDYTTSPLTKNGRIFYAIGCGAL
                     TVIIRLIGGYPEGVSYSILFMNVMTPLIDKLTMPKAFGKVKK"
     gene            14604..15182
                     /locus_tag="PK015_03565"
     CDS             14604..15182
                     /locus_tag="PK015_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HOL33581.1"
                     /translation="MKLFLRLGLTLLGFCAVATALLAYVNSMTKPLIDEHQRTAAEQT
                     MSKLIPDAVFEPVTIHTEKDSLVYYIAKDQKTGEIKGYTFTAVKPGYSSMVKTMAAID
                     TNFKIINIQVIEQSETPGLGANCTKPEFSAKFTGLSADDLVVDRDGGKFVSLTGATIT
                     TRAVTNSLREAIKLIQKDVESKTTTPSGEAQK"
     gene            15179..15772
                     /locus_tag="PK015_03570"
     CDS             15179..15772
                     /locus_tag="PK015_03570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HOL33582.1"
                     /translation="MSFMKELTKGIVKENPIFVLVLGMCPTLATTTSVNNALGMGLAA
                     TFVLVCSNIIISMIRNIIPNKIRIPSYIIVIAAFVSIVDMVMAAYLPDLHKALGLFIP
                     LIVVNCIIMGRAEAFASKNSVMDSMADGIGMGIGFTLALILMSSIREILGSGTWAGMK
                     VMPVTYDPMLVAILTPGAFITLGLLMAGINMLKEKFL"
     gene            15769..16371
                     /locus_tag="PK015_03575"
     CDS             15769..16371
                     /locus_tag="PK015_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HOL33583.1"
                     /translation="MNTLSQILVLAISAIFIQNLVLSRFLGLCPYLGVSTKMDSAFGM
                     GMAVIFVMTMSSAFCSLIYKYLLVPYNLTYLSTLAFILTIAALVQFVEMFILKNAPSL
                     YKALGVYLPLITTNCAVLGVAIINITPLPDGSTYNFLFATLNGFFSGVGFTFVLLVMA
                     AIRERLALVDLPKSMHGFAHGMILAGTMALAFSGFVGLKI"
     gene            16382..17416
                     /locus_tag="PK015_03580"
     CDS             16382..17416
                     /locus_tag="PK015_03580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HOL33584.1"
                     /translation="MNLSLLIPILAPSLASDIGVPVLIIGAMGLAFGLLLAFASKVFA
                     VTRDPRVEEIISVLPGANCGGCGKAGCAGYAEAIVLQGAPVNLCTPGGPKVAQEIAKI
                     MGTEAEAVVKKIAVIHCSTGGKDNTKWKYDYRGIETCLSAVNIAGGPNSCSWGCVGFN
                     DCLNACKFDAISVDESGMRIIDIEKCTGCGACVKACPRKLIDLISIQQNVFIKCSSKD
                     KGPLARQVCGTNQPCIGCGICVKKCPQQAITMANNLASIDYSKCIDCGVCAAVCPTNA
                     ILDTLKGTRKKSEIDAELCDGCHICVPVCAVGAIKGEENQKHSIDQDKCIGCGVCITV
                     CPKNAIKAKE"
     gene            17573..18922
                     /gene="gcvPA"
                     /locus_tag="PK015_03585"
     CDS             17573..18922
                     /gene="gcvPA"
                     /locus_tag="PK015_03585"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HOL33585.1"
                     /translation="MSYIPNTDAERNQMLKKIGVHSFEELIQVIPKKFRLQELLNLSP
                     PLSELELTQLVNALASKNIDTQKFISFLGAGVYDHFIPAAVDTIVSRPEFYTAYTPYQ
                     AEVSQGTLQYIYEYQTLICELTGMGISNASMYDGATAVAEAILMAVRKNHLKKAIISS
                     AIHPEFIKVIQSYTEGIGLELVFCDIKYGRTDIANLADKMDENVSSVVIQTPNFLGNI
                     EAMNELEKVVHSNPKTLFIASVDPVSLGILKPPSSYHADIVTGEGQALGNNMYMGGPL
                     FGFFATHKDMARLMPGRIVGATVDVEGNRAYTLTLQAREQHIRREKATSNICSNESLC
                     TLAATVYLSLLGKDGLIEVATQSAQKAHFLANEIASIPGFSLCYPETPFFKEFAIHCP
                     VSAQKVIQDMLNKHIFAGVDLKSYGYDENTLLIAVTEKRTRAELENYATALREMNYE"
     gene            18915..20369
                     /gene="gcvPB"
                     /locus_tag="PK015_03590"
     CDS             18915..20369
                     /gene="gcvPB"
                     /locus_tag="PK015_03590"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOL33586.1"
                     /translation="MSKTIFELSVPGRKSITLPPRDPDIPLNEIIPQELLREKPARIP
                     ELSEIDVMRHFIPLSQKNHCIEKGLYPLGSCTMKYNPKLHESLVRYPSFQNLHPLQPE
                     STLQGTLQILYELQNDLAEISGLDKVSLQPAAGAQGEFTGIRIIFAYHRAKGNTHKNK
                     IIIPDSAHGTNPASCALVGYEVVELKSDSRGRCDLAHLKSIVNENTAGFMLTNPNTLG
                     LFEDQIEEIASIIHSVDGLMYLDGANLNALMGLVKPGEIGFDLMHFNLHKTFATPHGG
                     GGPGSGPVGVIAKLAPFLPVPLIGKNEDGYFFDYSHKQSSIGKVHTFYGNVAVMLRAY
                     FYIRTLGAEGLRQVAENAIINANYLMALIKDYYHIQYPDYCMHEFVADSTWQKKEYGV
                     STLDIAKRLLDKGFHAPTVYFPLIVPEAMMIEPTETESKDSLDAFASAMIEIAQECKE
                     NPELLHSAPLTTPVRRVDDVRAVKMLDTSFPLED"
     gene            20401..21015
                     /locus_tag="PK015_03595"
     CDS             20401..21015
                     /locus_tag="PK015_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOL33587.1"
                     /translation="MKKILLLIVLCLGLLSLSFAAEVKIGVVKMDRIIQESNELAEIS
                     RIYKLDMQNWKNQLNELDAQIKQMEQEFEIAKLTKTEAAKREAQAKIDEKKAEAGRLL
                     EEYFGEGGKSEQRYMELLSPINTKINNIIKKIATDEGYTIIFDDSMGTVLYSAPSIDI
                     TDLVLLELNKDTVKPTPDNKTNTTGTQTPKPEDTFKPGEFIPKK"
     gene            21012..22055
                     /gene="lpxD"
                     /locus_tag="PK015_03600"
     CDS             21012..22055
                     /gene="lpxD"
                     /locus_tag="PK015_03600"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOL33588.1"
                     /translation="MKKFNKTITPEDIVAVCGGEYHGEQNITLQSVADPDEADSSCVI
                     FWEQEKYLDAVKKSPAVLIFCHPDKANNLPNRNLILHPHPYFAFLRLVDWWIEQDAEK
                     PIPEIHPTAIIDSSAIIGDGVYIGPYCIIGKNCQIGNDSIIEAHCFISNNVSIGKNTH
                     LYPDVNIYADTRIGNGVIIHSGTVIGADGFGFILMDGVQNKIPQVGNVVIEDFVEIQA
                     NSAVDRGTLSSTFIGQGTKIDNFVQIGHNCHIGKHCILCAQVGLAGSTILGDYVYLAG
                     QVGVAGHLKIGDRAMVGAQSGITNDIPADARYWGSPAMEADRFKRILVSQKHLPEIYR
                     FYLKQKKKEEEET"
     gene            22058..23449
                     /locus_tag="PK015_03605"
     CDS             22058..23449
                     /locus_tag="PK015_03605"
                     /inference="COORDINATES: protein motif:HMM:NF009667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HOL33589.1"
                     /translation="MTEYKNTIRTPVSYTGIGLHTGEISTITFKPARKDEGIVFIRTD
                     LPDKPEIPADIDHVVDISRGTTIGVKDATVATIEHVLAAIKGLSIDNIRIEIDGPEAP
                     VADGSAIVFLNLLKKAGLVQQDAERVYFEFDEPISYSAPEENVDIVIVPSNELKVTFM
                     IDYKHPYLGTQYTWLPDIKHFEKDFAAARTFCFINEVLELKEKGLIKGGSLDNALVIA
                     DPDMTEEELQHIQEVFGYHEKVTISPQGILNNHPLRYYNEFVRHKVVDLIGDIALLGV
                     PIKGHILAARSGHKTNVELVKKLRQMQLTQELKKQYQKDNSKDVIFDANAIMRIIPHR
                     YPFLLVDKIIDFKPNESIVGIKNVTINEPFFQGHFPGHPIMPGVLIIEGMAQTGGIIL
                     LNQMDDPTKYVAYFASIDNVKFRKPVMPGDTLRYEMRVISLKPNLSRMRGEAFVGHDK
                     VAEGDFMAILQKK"
     gene            23452..24225
                     /gene="lpxA"
                     /locus_tag="PK015_03610"
     CDS             23452..24225
                     /gene="lpxA"
                     /locus_tag="PK015_03610"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOL33590.1"
                     /translation="MTRIHPTAIIEKGAIIGEDCEIGPYCHIGPEVILGDHNVLSSSV
                     VIMGKSEIGNENHIFSYAVIGTDPQDLKYRGEPTGLKIGDRNIIREFVTINRSNQEGE
                     FTIVGNDNLLMEYVHIAHNCHIGSHCVVANTVQMAGHIIVQDYATIGGLTAIHQFVHI
                     GTYAFVGGASAVKKDIAPYTRGQGNPYKTVGLNTVGLTRRGFSNEAQNAIMKVYKLFY
                     SEGLNTSQALAEVETWSDLTPEQKVFVEFIRECERGLSR"
     gene            complement(24914..25207)
                     /locus_tag="PK015_03615"
     CDS             complement(24914..25207)
                     /locus_tag="PK015_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33591.1"
                     /translation="MDTPDPNSDNFMIWHFTTQAQEALEPPSLRIEKVNNQIKLSWDA
                     VQGATSYKIYGASDPYGNYTLITSTTSLNYIITNPGNMQFFKVTATSEKFLKL"
     gene            complement(25304..25471)
                     /locus_tag="PK015_03620"
     CDS             complement(25304..25471)
                     /locus_tag="PK015_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33592.1"
                     /translation="MPEHVTLISPPDNSVGLDPNNVILTWVPSPYGSMAAYYGVYVAP
                     DPETLFDSYYF"
     gene            complement(25616..>27096)
                     /locus_tag="PK015_03625"
     CDS             complement(25616..>27096)
                     /locus_tag="PK015_03625"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HOL33593.1"
                     /translation="QGILVGDDGLVALTNDGGASWTWPQSAQNIIGTTRMYGAVYFPD
                     GRIWICGYEGKVAYSPDFGVTWSLQAQGVTTVACYGISMNESGVGFVALNKGTPSQSK
                     ILRTTNFGQTWNLINLTVTDNPSFNKVRQFGNKVVLCGDKGCISYSNDNGVNWTHYYG
                     AGGTGTSMRDVVMEGNVGYAVGRNATIIKTTDGWATWQLLNHNATVYIEGIKFRNDGS
                     LLACGWQGTLMLSTDGGITWEDLVPNAIDLWQADVLDANNWYIVGDKGTILRTMDGGH
                     TLIKKNISGTTSLFYTCYFKNVNEGWVTGKTSGNIYHTVNGGDNWSVFTIPGVSSAKA
                     FYKIFFVNDQVGYIVGLGGKVTKTTDGGETWFTVGDNISTSANLYCTYWKNELNGYAG
                     SSGGVLYITNNGGVTWTSITVGANANIRDIYFRDANNGVLVKDGGEIYYTTTGGNTAA
                     SWLAATEQAIGHMMSVTCDHNGVFWAAGYSSNPSQQGNSWAL"
BASE COUNT         8679 a         4962 c         4995 g         8360 t
ORIGIN      
        1 ggctggaact atgtgatttt acctaaaact ggagatagtt tattaacctg tttttctgct
       61 tctgtggtgc tgcaacttct ggcattaaag atgagtgtgc gcaagatgaa aaaactggat
      121 aaactcaatg tgaaagacca tggagtgcat ccagatgttc ctaaaaatgt ttccaaatcc
      181 ataacggttg attaaagttt atgttacgca taggttacgg ttacgatgtg catcgtttag
      241 tagctggcag aaaactgatt ctgggaggag tggaaatccc ttttgataaa ggattagaag
      301 gtcattccga tgctgatgtg gtcctgcatt cagttattga tggacttttg ggtgcattat
      361 ctctcggtga tataggttca catttccccg atactgaccc tcaatatgag ggcattgata
      421 gtcgtattct tcttcaaaga actttttctc tggtgcaagc agaaggttat gtccttaata
      481 atctggattg cactatttgt gcagaaagtc caaaacttca accctatctt gataagatgc
      541 gtaaaaattt ggcaaaggat ttagcctgtg atatatcgca aatcagcctg aaagctacta
      601 cggaagaagg tttaggagta tctggtaacg gtggtatttc tgccacctgt atcttattat
      661 tgaggaaaca atgaaagcta ttggcgtttt tggttatcat cataccggga aaaccactct
      721 tgcatcaatg cttatctctg ccttaaaaga gagaggatat gaagttgctg ctattaagga
      781 tattcataat gaaaactatc attcagataa ggaaggttcc aattcctggc gtttagctga
      841 agcaggggcg aatccagttt tcgctaaagg actgtatgat gcagctttaa tttttcaccc
      901 cgctccagaa ttaccagata tgcttcattt tctaaacgct gattggttgg taatagaagg
      961 ttttcaagaa gctgctctgc ctaaaatttt atgtgcggaa aacctcggac aaataaaaga
     1021 actttggaac ccaaccgtga tagctatcag tggagaaatc tcacaaactt tacagaacta
     1081 tcaggataca cctgtaatag attataagac agattttaat agaatcctga atctggttct
     1141 ggaaaaagca tttgatattt taccacagag tgacccggag tgttgttctg cttgtggaaa
     1201 gacctgttat caaatgacta tagacatctt gcagggtaaa gcaaagcgag aagattgcgt
     1261 gctggataac cataattcca tctctattag cattggtggc aaaccattga ctattgtacc
     1321 tttcgtgcaa aatcttttcc gggataccat tattgctatg atttccaatc ttaaaggggt
     1381 taatccttcc caagatattg aaattaggat aaaaggtaaa gatgactgaa ctctttaatg
     1441 aactctttgc caatcaaaat ccagaatatg tgaaaatctg gcaaaattca gtagtgggta
     1501 ttgctggagt cggaggtctt ggctctaata tcgctttttc tctcactcgt gctggaatag
     1561 gaaaacttat tatagccgat ccagacattg tttccacttc aaatttaacc aggcagcagt
     1621 attttttgaa tcaggtgggt ctaccgaaag tggcagctct aaaggataat ttactgaata
     1681 ttagtccttt cactatggtt aatccttatc cacaaaagat tacacctacc aatctttctc
     1741 tcatttttgg caaggtggac atacttatag aagcactgga tgaagctgaa gagaagcaga
     1801 tgcttataga aaattggcaa caactctatc ctgataaata ccttattggc gcttctggta
     1861 tagcaggagt aggaaaaaac gaacttatac atattgagca gatagggaca ctttttctgg
     1921 tgggagatgg aatcaccgag atagaaaatg gagtgtatcc tactgctgcc agagtggctg
     1981 tagttgctaa tatgcaggca aatctctgcc tggaactttt gctccaaaaa ggaaattgat
     2041 tatgccaaca attacagtta atggacaaga aattccctgg gaagaaggaa tgaccataag
     2101 aactgtcctg aataaaatga attatagctt ccgaatgctg gttatcaaag taaatgacaa
     2161 actggtaaaa aaagaggact gggatagttt ccaagtacct ccgggagcag atgtcaaagt
     2221 tatccatctt atcagcggtg gttgattctt acctctcttt ttaaatcatc tttttatgcc
     2281 caaaactctt tccttgcttg aggtctataa tattttactg aatttccaag gtccacaaca
     2341 ttggtggccc ggagaaactc aagatgaaat cattataggt gctatcctca ctcaaaatgt
     2401 gagttggaaa aatgtggtta aggctcttaa taaccttcat tctgccggaa tttatacctt
     2461 gaaagacctt gatgctctgt cggttgaagc tatagcacct ctaattcggt ccacacttta
     2521 ttataatcag aaagctataa aactcaagac ctttactaat ttcctccggg agagatacca
     2581 atttaacctc cagcagatgt ttgccgaaga cctaaaaact ctgcgttcag aaatgctaaa
     2641 actgaaggga ttaggtccag aaactgttga ttctatcttg ctttatgctg gttcaaaacc
     2701 tgtttttgtg gtagatgctt acactatccg tcttttaact cgcttgggat tcaccgataa
     2761 aaagatgaac tactcccaat ggcaagaatt tattaccgaa agaattcccc aagatgtaga
     2821 actttataac gattttcatg cccagatagt caatctatgt aaggatattt gtcgcaataa
     2881 accccggtgt gaaatttgtc cccttacctt atattgcact tattatcagc aaaataaaga
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggtgtgaaat ttgtcccctt
     3061 accttatatt gcacttatta tcagcaaaat aaagaaaatc ctgtcacttc ttcttagttt
     3121 ttacgataat tttctctccc ctgcctttaa atatatagct aatgccttac tcatatttaa
     3181 tttatgtcca gtttcggaca taagctggac atgagttgga catgagctgg acataagcta
     3241 cccatctgga ggcaagaaat tggaatatga taaccggaat ttttatcctt ttatatcatt
     3301 ctaacatcaa tatacaaaag gggttggata aagcattgaa aaaataacta accgaggata
     3361 aacttgacag aaattctcat tttatttttg caattatctt atgataaatt acactgatct
     3421 attttagtaa atgcccactc tggcaaaata gacagtttaa ggaggtcaaa ttggggtcta
     3481 ataattatac ccttttaaat gttcccttca gtagcaatct gatagattat gcgttaacgc
     3541 aggtatcaga tagatgtatt tttgttttcc ccagcaggat atctgcagaa aaagcacgag
     3601 aaaaattctt gccaaattgg aaatttgaag cgtgtgaatt catttctatt gaggatttta
     3661 aggattggtt aattcttcct tcagaaccac ccttaaccga tgaaaaacgt ttactttgcc
     3721 tctatcaagc actcacttta gaggataaaa agtttttcaa gttttacact tactttgatt
     3781 taatcagttg gggtaataat ttcttccaat tttttgaaga gctaaacgat gaagacatcc
     3841 ttccagaaga gattgagaat agaatagatt tgcgggaatg gcagcaagat tttctggcta
     3901 aaattttaaa tatacgagaa aattaccaaa agctacttga acaaaaaggg ttcacagacc
     3961 ctatatttta tcgtaaagct tcccagctaa agataccatt ttccggttat aaggtcattt
     4021 gggttaatca attttactat tctaaactgg aacttgccat tcttaaagcc ttagctgaat
     4081 cagaaaatga ggtggtggtc atctctcaaa cagaaaatgc tgattttaat cctcaaaaca
     4141 gaaaaaatac ctctttaaat ctggaaaatc tggctaaaaa tgactattta actcaaaaaa
     4201 ttaaggtaat agaaaccgaa aatgaagacc agatggttat ttcttttttg ggtaaacttg
     4261 gtaaagccaa aaatatagaa ggaacaactg ctatcattga tagccattac tgggataaac
     4321 actatcggaa tttgatgaat ccagctcttt ttgactttag caactcaatt aatctaataa
     4381 attccagccc ttatcaattc ttgaatatct taaaagagca cttgcaagct ctcactaccc
     4441 tttcttttga agggaaagaa ataacagctc ttcccatcaa actaatgctt gatgcagtag
     4501 gaaatgaaag ttttgtacat tattactttc caaaggatac aattcaatct aaagaagaac
     4561 tgctaaatga attgaccact ttgaggaaaa aagatgtcta ctggctggat aaaaatatga
     4621 attgcctcca agtttttcat acttcccccc attttaataa attaacctct ttagtggaaa
     4681 atcatttttc tttgctggaa gaaatcgcta aaataaatag ccccgctgat ttgattaatc
     4741 tgatagatac tgaagaaatg tctattggtc tgaatattaa aaggatgtgt agtgaaaacg
     4801 aaattcttca tagcgacctt ttagagcagt tttatcaaag aatagctaat tttgccagta
     4861 ccgataatct acaattagtt caggattgga attccttctt tgcttcatcg ggtaaggatt
     4921 tagctttaaa tgtcttccaa ctttttctgg attatcttga aaatgctggt tataaattct
     4981 tttatgaaat tgataaagaa aataatccta ttactatcac taatactatg gattcacgca
     5041 atctacgcta tgacaatctt gttatctttc acgccattga aggtgaattt ccctctgctc
     5101 ccaaacctgt ttggctatta aatgaagctc aaaaaggtca acttggtctt aaaacttata
     5161 atgacttgcg agaatgggaa agatactatt ttttccgtct gattttgacc tccagaaatg
     5221 tccttattta cacctatcgc aatagtgaat tggatattga acctggaagc tttgtaactg
     5281 aactacaaca tttaaatgaa aaccacaaac ttaatgagct tgagtttgat tgggtctcct
     5341 cagaggtccc tcttagtaag tactataatg caagacaaca aaagtattct tctggtgaaa
     5401 ataatctttt agcctcttat cctgattatc tattaaatag agaagaaaat cctcagattg
     5461 atgagaaaga tttctttatt cttccttgcg aacctacgaa ggattttgga gaaaacttgg
     5521 aactcacctg ttcttattac aatctgcagc agcttctgga taataccttt gtctggtata
     5581 tccaatggat acagcaatta caagaaatag attttagacc taaggatacg gtttcagcta
     5641 aaatagttgg ctccctggtt catagattta tttctgattt gctttcacct tttaacggaa
     5701 agccttgcaa gattgaagaa cttcaagaat tgttttatca gcctgtgaca acgctggctg
     5761 aatattttag agacttattc acttcaaaag atttcttttt ccagctacct caaaattata
     5821 gccttaaatt cctaaaagat atctatacca attatctgcc tctatctatg caaagtttct
     5881 atgaggattt cttatcaact aacttagcag ataaagtttt tactttattc gctgaatcag
     5941 gaatatatga tgataattta actcaagaac atctcctacc cttacctcca gataatcaag
     6001 aatcagaaat aaaagttata cttcggggac gatcagattt aagaatagaa actgatgata
     6061 aaaaatttat cgtagatttc aaaacgggaa gaaatccaga taaagggcaa ttattattct
     6121 atgaatggct atattacaga tggcaacaag aagatatgga catctctccc attttctgga
     6181 ttattaatga taataaaatt atagaaaaaa caaaatatga tgatgcaaat aaatggagaa
     6241 atacgattta tgagactctt cataaatgta gagctgaagg atataaatta cccaagacag
     6301 taactcaact caaacaattg aaaagaatta ctcgagctga cctttatcgt cctaaacagg
     6361 gaggaaacaa tgaataattc tactattaaa attattaaag ccagtgctgg aactggcaaa
     6421 acctaccgtt tagcagttga atatgttaaa ctcgtacttc aatatggaac aaatataaat
     6481 ctggattcta tcctcgtcct cactttcact cgcaaagcca ctgccgaaat tagagaaaga
     6541 atcgttgaac atcttgagct tttaaactct actaaagata aagaaaaaga agcgaaattg
     6601 gctcttatta aagctatttt tggcaatgag aataagacgg aattgcacga tatagaaaag
     6661 aaaaacttac aagaagctct gcaaaaaatc aagactgacc accaacaact tcaggttatg
     6721 actattgatt cctatatcaa caacatcttc cgcaatttag tcaatccgca aaaaaactat
     6781 ggagattttg aaatagatga tgatatcgta gaaaaaacct tacccttact ctatcaatat
     6841 cttcttagct ccaatttaga actttaccaa aaagtagaaa atcttctcaa agccagagtg
     6901 acttcctcaa ttgatagata taatttattt ttccaatccc ttatccagaa ccgcttaatg
     6961 ttacacttaa tcaatgtaag ccaaaatcac tttgatcccg attctttgat gtatctctgt
     7021 gagcacgatg aaatatgtga agaaaaggct aaagaaagca ttgagaaagc taaaaaagaa
     7081 ttaattaact atatcttaga tataatgata atagcagctg ggaagaattt ttccaatata
     7141 tttatagttg attttcaaag gttgttcaag gaagtcccca ctacttttga tgaattaata
     7201 actaagatag aagaaatgct taatccaact gatgatgcta tacagcaaca aataagggat
     7261 gaacaaagtt ataaattatt aaaagtatgt ataaaaaaat tgaaggataa taagaatatc
     7321 tacaatgcta aaacccttac ttctaaaagc cttggcaata aaactattga gtggaatgat
     7381 tatttaaatg aagtaacaga acatctatca gactactttt tattcaaaaa gtttattcct
     7441 gaacagcgtt atatcctcag tatctggaaa gaagttttag aggaatatga caaactactt
     7501 tatagtaaca agaggttaac ctataatgat gtctcctggc tcactctgaa cactctttta
     7561 aaaggtgatg tggaaagtat tgattgggat aatagtgaga ttgataatga attctatttc
     7621 tttttgaccc atcgcactcg ctatcttttt attgatgaat ttcaggatac ttcacttttg
     7681 caattcttta ttctaaaacc tattatgctg gaattgagtt ccggagaagg ttcggaagaa
     7741 tacggaaaag ttgttgtggt tggagatgaa aaacaatcca tctttagctg gagaggtggt
     7801 gaaagagact tgttacttaa tttaaaagat atcctgccaa ctatagataa atcatctgga
     7861 gtagaagaaa cacttgagga atcttatcgc tccagtaaag caatgatgga ctttataaac
     7921 tatgtctttt ctaatgaagc acttattcag gacttaaaat tgaaaaatct ggattgggaa
     7981 tataacgaat gtaaagccgc aaaaccacaa tgttatccta ctaatataga actatctctc
     8041 cagcgctatt cttctactga ggaagatttt aaattggata aggttcaaag agaatttatt
     8101 cagaatttca taaaacctgc ctgggaacaa aataaagata ataaagatta tactatggct
     8161 attctttgtc gtactaatcc acaattggaa acttttcaat tacttctgga agaagccgga
     8221 attgaaagca tctaccaacc cagcagtctg attacagaac ataattatgt ctatccgcta
     8281 atttctttac ttaaattcat taattataaa gactggttag agttcctcac cgttctgcgt
     8341 tctaactata ttatgcttaa agccgaaccc ttgaagaaag ttataactat tatcagccaa
     8401 tctttaaaag atttcaccga accagacttt tctgaatttc ctgtagttca gaccttatat
     8461 gaatttcaca gaaaagacag ggataaaata tcagatattt gtcaggattt tttggattta
     8521 ttcttatata ataaaaagct ctctgaacga gattatatta atatcaatgc ttttatggct
     8581 atactacaag actatgaact taataatacc gataaagcta aatccatatc tgcttttctg
     8641 gattttctgg acgataacaa agctcaagat tttatgaaac aagttccctt aactggagac
     8701 atacctctgc aacttttaac tatacacaaa gctaagggtc tggaatttga ccaggtattt
     8761 gtcttctacg acctttccgc ttcgcataat cctgaaagct ataacctttc tttatatcca
     8821 aaatataaag ataagacctt tgaaaaactc agtgatatcg gtatcacctg ccactacgat
     8881 gacatactta aatattccag caaaaaagaa ttggttgaat tagatagtaa aaaagaaatg
     8941 ctggaagaaa tgaatactct ctatgtagcc ttcacgagag caaaaacttc cctgagttta
     9001 tgtatggctt atgaacataa gaaaggtttt gatgatttgc ttaacaataa acaagagagt
     9061 gataagtact tgcctatact tcttacaaaa atagtccgag atttctttac ttctaaaaat
     9121 attgaaccta ccggaccaga tgaaaatatc tataaattcc ctgttgcagc tgaaactact
     9181 ccaaaaccta aagaagaaaa ggaatcaaat aaagaaaggt tatgtgcttt agatttaacc
     9241 actgtcctac cgcctaaaca accatatcct tttaaagaaa ccgttgagac ttctaaaaat
     9301 aagtacctaa actggaaaag tatgtggctg gaaaatcgca gcaatctcta tggaaacctt
     9361 gctcactact atctcagttt cctgaaatat aatcttcctg aagaacataa ctatgcttta
     9421 aaacaatgta ttttaaagta tggcactctt ttaactcatt ccgaaataga ggaaaaaatc
     9481 aattccctga aaaacaatct tcctatctca gaattgttcc ctcccgatta tgataaggtc
     9541 ttcaccgaat ttactctctt ctctgaaggt aatcaatatc gcatagatag attgttaatt
     9601 gatactaaac aaaaacgagc tctaattctg gattataaaa ctggggtaac caaagaacaa
     9661 gaacaattgg aacactataa aaatgcatta tctaatttgc ctgctatcaa agatgacaac
     9721 tttagtatag acacaaaatt tatttcgtta acattatagt gctctctttc tctcctaaat
     9781 tgaaagctgg agatttactc tccagctttc ttaatttaca aatatgcaat aagcaatgtt
     9841 tcttttatta taaatataag ccaagaaaga caatccccta aaaaatttag gtttacctga
     9901 tgaactctcc tcacgatgga cttataaaag ctaaacttga attgacttct taatctcata
     9961 aaagtaaaca aaacaagaga gctatactaa attgatacta tttataaagg taaaatagca
    10021 gactgctaag ttaaaaagac ttcaaaaagg aataataatt gaaggattaa gctaattgta
    10081 agagtttatg tagctgtaat tgtaaatggg caggcactcc atcttttagc atccattgag
    10141 cgagagtttc tggagcaaga cgaccaatca caggagaaaa aagtaaaatc ctatcctcta
    10201 aggcattatt tcggataaaa tttagggcaa acatatagtc ttcatgcgaa gcaagaacaa
    10261 actttatttc atccactggc aggagaaaat taaggttttc cattaaaaat gattcaccaa
    10321 aaccacttcc aggacacttc acatctacga ttttcactac ataatcaggg acattttgca
    10381 gggattgagt accattagtc tcaagtaaaa tccgatattt ctcctgatgc aatatctcca
    10441 tcagttcaat tgttcttttt tgtaaaagag gttctcctcc ggtgatttcc actaaagaac
    10501 aaggatattt acgaatctct tgaagaattt gttcaatgga catttctttc ccattgctcc
    10561 atgcataagt tgtatcacac caggaacaac ggaggttaca tccgcttaat ctaataaaaa
    10621 tgcagggcaa accataatag gttgattcac cctgcaaact atagaaaatc tcacatatat
    10681 ttaatgacat agaatacctc tcacttttca agaaaaagag acgccactta tttctgacga
    10741 atttctttca cttttttagt gattaaggga actatctcaa acaggtcgcc tactattccc
    10801 aggtctgcta ctttaaagat aggagcatct gggtccttgt taatggctat aatataatcg
    10861 gaagaagaca ttcctgccaa atgctgaata gcaccagaaa ttccgacagc aatataaatt
    10921 tttggtttaa cggtctttcc agtttgaccc acttgatgag aatagggaat ccattctgca
    10981 tccacagcag cacgagaagc tcccactgcc ccacctaaag cttcagctaa ctcttctaac
    11041 agagcaaagt tcttagcttc tttcagacct cttccacctg aaacaatgat atttgcctcc
    11101 gtaatattaa cgagagtggt cttttctttt tcaaaacaca accagaagct cttatcttct
    11161 aaatcactaa aatctatctg ttctttaatt atttcacctt tgcgtgaatc atcacgaggt
    11221 aaagcatcca taactttatg acgaacagtt gccatttggg gacgatgatt aggagtgata
    11281 atagtagcca taatattgcc accaaaggca ggtctggtct gaagtaagtt tccagtttcg
    11341 ggatcaattt ccaaaccagt acagtctgct gttaaacctg tatgtaattg taccgctact
    11401 ctcgggatga aagaccttcc ggtaacagtg gctccagcca gaataatagc tggtttatgt
    11461 ttacgagcaa gttcagttaa agctcgtgca taattgagat caagaaaatg ttccagtgct
    11521 ggattatcaa cagctatcac ctgatctgcg ccaaaagcaa aaagctctgg atacaggttt
    11581 tccagatttg agcccagaag cacagcagtg agagcagaat tcatttgttc agccagttct
    11641 tttcctttcc ctaaaagctc aaataccaca ggtgcaataa ctccatctct ctgttcagca
    11701 aagacccaga cacccttata ttgttcctta tcaatttgtt ctgctgcttg ttttcggata
    11761 aggatagcct caaaaggaca agtttccaca catgctccac aaagtgtgca tttatcttca
    11821 tctataacag caagttttgc ctgtatggtg attgcaccat aagcacaagc tctggtacag
    11881 agtccacaac ctatacattt ctgatcaata atttcaatca tcggaaaact cccagataac
    11941 caagatttga atcctaaact tcactattaa actcatatat ttgcgagcaa tatagcttca
    12001 taagggctat aatttttaat cagtagaatt gagtcaagga aagtctaaca ttatttctga
    12061 aaaaaaatac ttgcctaaaa atgatattta catttataag ggaaaaaata gaaatctccc
    12121 agagagcaaa aaggagtgta aatgaaattg aaaacatttc ctgggggtgt gcatcctccg
    12181 gataacaagc aatacacttc tgctgtcccg attgaggagt tcccccttcc tcaaaaagtg
    12241 gtaatacctt tgtctcaaca tgtgggagct ccatccgtgc cactggtaaa agttggtgag
    12301 gaagttaaaa ccggacaaaa aattgcagaa gcaaatgctt atgtttctat ccctcaacac
    12361 gcttctataa gtgggaaggt aaccaaaatt gataccttcc ttcatccttt aggagcaatg
    12421 agtttagcta ttgagattac tggtgatggc tcagatagct ggatagagct tgtggatgag
    12481 ccagatttta tgaacttgcc tgtggaagaa atgaaaaatc gtatcaaaga agcaggaatt
    12541 tgtggtatgg gcggtgctgg ttttccaact atcgttaaac tctcaccacc tgaaaacaaa
    12601 cctatagaca ccgttattct taatggagtg gaatgtgaac cttatctcac agcagaccat
    12661 agattgatgc tggaacaatc tgaagggata atcaatggat taaaaataat tatgaaaatc
    12721 ctgaatgctc aacacggaat gattggcatt gaatccaata aaccagatgc tattgctcta
    12781 atgaacaaac ttttagccag cgaaaaaaat cttagtgttg tccccttgaa attgaaatac
    12841 cctcaaggtg cagaaaagca attaatctat gctgctacaa aacgtatagt tccagctgga
    12901 ggtttacctc tggatgtagg aatagtagta caaaatgttg gaacagcctt tgccatttat
    12961 gaagctgttc gtttcaagaa acccttagta gaaagaatta taactgttac cggaagtgta
    13021 gtaaataatc ctaagaacct taaggctcgt ataggaaccc tattctctga tttagtaacc
    13081 tattgtggtg gaactaaatc ggagattgga aaagtgattt ctggtggtcc tatgatgggt
    13141 tatgctttac cttctctgga tgcccctatg gcaaaaggca cttctggttt gattttattc
    13201 agtgaaaaag aagccaggac cattgaagaa cgcaattgta tccattgtgc tcattgtgtt
    13261 gatgtgtgcc cgatgaatct tgtccccagt cttattgctc aaggagttaa gggtggagac
    13321 ttgacacttg ctgttaaagc gggtctggaa gattgtataa aatgtggtag ttgtgcttat
    13381 gtttgtcctg ctcatatacg aatagttcag tgggtagaca caggaaaatt gcgttatgca
    13441 gaagaacgac aagctaaatg aatataggat tgaattatga aagataaatt tattgtttca
    13501 cctgctccgc atatacatga ttctaccagt attccaaaag ttatgtggaa tgtggttata
    13561 gccttacttc ccgctttagc tttctccatt tattattggg gttggaagac cctttttctt
    13621 actgctttag gagtgcttgc tgctatttta accgaagctt taatccaaaa attacgtaaa
    13681 gtacctatca cagtgtatga tggatctgcg gtagtaacgg gaatgctttt aacctataat
    13741 atttctcctg gttctccctg gtggttgcct gttataggtt caatttttgc catcgctata
    13801 ggtaaacagg tatttggtgg tcttggtaat aatgctgtca atccagcgct tttaggaaga
    13861 gcttttctgc tggcttcctg gccttctttg atgacaggta attgctggaa gccaactatg
    13921 gctcataatt tatctgcttc ttccattaat ggaatcagca atttaagtgc catcagcgat
    13981 agtcttgcca ctgtttctcc taaagcctac aatttggtta ccggtgctac acctttaaag
    14041 gtgatgcaga ccttaaggga tactactttt attaatacct taaccagtga tccagccact
    14101 aatattgacc tcggaaccaa gatatttaat gggctcatag atatgggaac cttaaaatct
    14161 atgttctggg gaaatattgg aggatgtatt ggtgaagtat ctgtttttgc tttgctcatt
    14221 ggtgctatct atttactctg gaaaaatata attgaatggc gcataccctt cttctatctg
    14281 cttactgtgg ctgtattaac tttccttttt ggtggaatcc ctggtttgcc tgaaacacaa
    14341 tattcactta gtttacccat tttccatctc tttgcgggag gtttaatctt aggagctttc
    14401 tttatggcaa cggactatac cacaagtccg ttgaccaaga atggacgtat tttttacgct
    14461 atcggatgtg gtgctttaac tgttattatc cgtttaattg gtggttatcc tgaaggagtc
    14521 tcctattcca ttctctttat gaatgtaatg actcctttaa ttgataaatt aacaatgcca
    14581 aaagcatttg ggaaggtgaa aaaatgaaac ttttccttag actcggttta accttgttag
    14641 gtttttgcgc tgtggcaaca gcacttttag cctatgtaaa ttctatgact aaacctttga
    14701 ttgacgagca tcaaagaact gcagccgaac aaacaatgtc caaactaatc cctgatgcag
    14761 tatttgaacc cgtcactatc cacacagaaa aggattcctt ggtctactat attgctaagg
    14821 accaaaaaac tggtgaaatc aaaggctata ctttcaccgc tgttaaacct ggatattcca
    14881 gtatggtaaa gactatggca gcaattgaca ccaatttcaa gattattaat atccaggtta
    14941 tagaacaatc cgaaactccc ggtctgggag caaattgcac aaaaccagaa ttttctgcta
    15001 aatttaccgg tttatccgca gatgatttag ttgtggatag agatggaggc aaatttgtca
    15061 gcttaactgg agctactatc actactcgtg cagtaacaaa ttctctcaga gaagctataa
    15121 aacttattca aaaagatgta gaatctaaaa ctacaactcc ttctggggag gcgcaaaaat
    15181 gagctttatg aaagaactta ctaaaggaat tgtgaaagaa aacccaatct ttgtgcttgt
    15241 tctcggaatg tgtccaactc tggcaactac cacttctgtg aataatgcct taggtatggg
    15301 attagcagca acctttgttc tggtatgctc caatattata atttcaatga ttagaaatat
    15361 cattcctaac aaaatccgta ttccttctta tataatagtt attgcggctt ttgtctctat
    15421 tgttgatatg gttatggcag catatttacc cgatttacat aaagctctcg gattgtttat
    15481 tcccttaata gtagtaaact gcattattat gggaagagca gaggcttttg ccagcaagaa
    15541 tagcgttatg gattcaatgg cagatgggat tggcatggga ataggattca ctttagcgct
    15601 aattcttatg tcttcaattc gtgaaatttt gggaagtgga acctgggctg gaatgaaagt
    15661 gatgcctgtt acctatgatc caatgcttgt tgccatccta actcctggag catttattac
    15721 tttaggactt ttgatggctg gaatcaatat gttgaaggag aaatttctat gaataccctc
    15781 agtcaaatct tagtcttggc aattagtgcc attttcatac agaacttagt gctttccaga
    15841 ttcctgggat tatgtcccta tctgggagtt tccaccaaga tggattctgc ctttggaatg
    15901 ggtatggcag taatctttgt tatgactatg tcttcagctt tctgtagcct catttataaa
    15961 tatctcctgg ttccttataa tttaacctat ctgagcactt tggcttttat tcttactata
    16021 gcagctctgg ttcagtttgt tgaaatgttt attctgaaaa atgcaccttc actttataag
    16081 gctctgggtg tatatttacc actaatcact accaactgtg cagttcttgg tgttgctatt
    16141 attaatatca ctcccttacc tgatggttca acctataatt tcttatttgc tactttaaat
    16201 ggcttctttt ctggtgtcgg atttactttt gtgttactgg ttatggcagc aattagagag
    16261 agattagcac tggttgatct gcccaaaagt atgcatggct ttgctcatgg tatgatttta
    16321 gcaggaacta tggcacttgc tttttccggc tttgttgggc taaaaatctg aggagtaaag
    16381 tatgaatctt tctttgttga taccaatact tgctccttct ttagccagtg atattggagt
    16441 tcccgtgctc attattggag caatgggttt agcctttggt ctattactgg cttttgcttc
    16501 taaggtcttt gctgttactc gtgaccctcg cgtggaagaa atcatttctg ttcttcctgg
    16561 tgctaattgt ggaggttgtg gaaaagcagg atgtgctggt tatgccgaag caattgtttt
    16621 gcaaggcgca ccagttaatc tttgcactcc aggcggtcca aaagtagctc aagagatagc
    16681 taaaataatg ggaacagaag cagaagcagt ggttaaaaaa atagcagtta ttcattgctc
    16741 tacgggtgga aaggataaca ctaaatggaa atatgactat agaggcatag aaacctgtct
    16801 ttctgctgtt aatattgcag gtggcccaaa ttcctgttct tggggttgtg tcggattcaa
    16861 tgattgttta aatgcatgta aatttgatgc aatctctgtg gatgaatcgg gaatgagaat
    16921 tatagatatt gaaaagtgca ctggctgtgg tgcctgtgtt aaagcttgtc cgcgtaagct
    16981 gatagatttg atatctattc agcaaaatgt gttcattaaa tgttcctcta aagacaaagg
    17041 acctctggcg agacaggttt gtgggacaaa tcaaccctgt attggctgtg gaatctgtgt
    17101 aaagaaatgt ccgcagcaag ctataactat ggcaaataac cttgcttcta tagactattc
    17161 taagtgtata gattgtggag tttgtgccgc agtttgtccc accaatgcta ttttagatac
    17221 cctaaagggc acacggaaga aatcagaaat agacgctgaa ctctgtgacg gatgtcatat
    17281 ttgtgtaccg gtatgtgcag ttggcgctat caaaggtgaa gaaaatcaga aacatagcat
    17341 agatcaggat aagtgcattg gttgtggagt atgcattacc gtctgtccta aaaatgccat
    17401 caaagcaaaa gaataattcc tctcctctca ttaataaata taaggcagga aaacagttcc
    17461 tgcctttctt atgttctctt gtaaatgggc tgcaaagaat tttagaattg acataaacta
    17521 taacacaaaa aaagttagat ataatatact tttaattgca aatggagaga taatgtccta
    17581 tattcccaat actgatgcgg aacgcaatca aatgctgaaa aagattggtg tgcattcctt
    17641 tgaagagctg attcaagtta tcccgaaaaa atttcgcttg caggaattgc ttaatctttc
    17701 tcctcctctg tcagaacttg aattgactca attagttaat gccttagcca gcaagaatat
    17761 tgatactcaa aaatttatct ctttcctggg tgcaggagtt tatgaccatt ttattcctgc
    17821 tgcagttgat accatcgttt cacgaccaga attttatact gcctacactc cttatcaagc
    17881 tgaagtaagc cagggcactt tacaatatat ttatgaatat caaaccctta tctgtgaact
    17941 taccggaatg ggtatctcca atgcttctat gtatgatgga gcaaccgctg ttgcagaagc
    18001 tattttgatg gcagttcgta agaatcatct taaaaaagcc attatctctt cagctattca
    18061 tccggaattt atcaaggtta ttcaatctta tactgaagga atcggtttag agctggtatt
    18121 ctgtgatata aaatatggca ggacagatat tgctaatctg gcagataaaa tggatgaaaa
    18181 tgtgagttct gtggtaattc aaactccaaa tttcctggga aatatagaag caatgaacga
    18241 gctggaaaaa gtggttcata gcaatccaaa gactcttttt atagcttctg tagatcctgt
    18301 ctctttagga atattaaaac ctccctccag ttaccatgct gatattgtga ccggtgaagg
    18361 tcaggctctg ggaaataata tgtatatggg tggaccctta tttggatttt ttgccacgca
    18421 caaagatatg gctcgtttaa tgccaggaag gattgttggt gcaactgtag atgttgaagg
    18481 gaatagagcc tacactttaa ctttacaagc acgggaacaa catataagaa gagaaaaagc
    18541 cacctctaat atctgttcca atgaatctct ttgcacttta gctgcaactg tctatttaag
    18601 tcttttggga aaagatggtc ttattgaagt agccactcaa tctgcccaga aggctcattt
    18661 tttagctaat gaaatcgctt ctatccctgg tttttcactc tgttatccag agactccttt
    18721 cttcaaagaa tttgctattc actgtcctgt ttcagctcag aaagttattc aggatatgtt
    18781 aaataaacat atctttgcag gagtagattt gaagtcctat ggatatgacg aaaacacctt
    18841 gcttattgca gtaacagaaa agaggactcg ggcagaactg gagaactacg caactgctct
    18901 gagggagatg aattatgagt aagactatat ttgaacttag tgtgcctgga cgcaaaagca
    18961 taacacttcc tccaagagac ccggatattc ctcttaatga aattatccca caagaacttt
    19021 taagagaaaa accagctcgc attcctgaac taagtgaaat tgatgttatg cgacatttta
    19081 tccctctatc gcaaaagaac cattgcattg agaaaggtct ttatcccctg ggaagttgca
    19141 ctatgaaata caatccaaaa ttgcatgagt ccttagtgcg atatccttct ttccaaaatc
    19201 ttcatccttt gcaacctgaa tcaactttgc agggaacctt acagatttta tatgaactac
    19261 aaaatgattt agcggaaatt tctggtctgg ataaggtcag tctacaacct gcagctggag
    19321 ctcaaggaga atttactgga ataagaatca tttttgccta tcatcgtgct aaaggaaata
    19381 cacataaaaa taaaataatt attcccgatt cagcacatgg aactaatcca gccagttgtg
    19441 ctttagttgg ttatgaagtg gtagaattaa aatcagattc cagaggacgc tgtgaccttg
    19501 ctcatctgaa aagtattgtg aatgaaaata ctgctggctt tatgcttact aatccaaata
    19561 ctctgggctt atttgaggac cagattgaag aaattgcttc catcatacac agtgttgatg
    19621 gtttaatgta tttagatgga gcaaatctta atgccttaat gggtctggtt aaaccgggag
    19681 aaattggatt tgatttaatg cattttaact tgcataaaac ctttgccact cctcatggag
    19741 gaggaggacc tggttctggt ccagttggag ttatagctaa attagctcct ttcttacctg
    19801 ttcctttaat tggcaaaaat gaggatggat acttttttga ttattctcat aaacaaagtt
    19861 ctatcggtaa ggtgcatact ttctatggaa atgtagctgt gatgttacga gcttatttct
    19921 atataaggac tctgggtgca gaaggacttc gtcaagtggc agaaaatgcc attatcaatg
    19981 ctaactatct gatggcttta attaaagatt attatcacat tcaatatccc gattattgta
    20041 tgcatgaatt tgtagctgat tctacctggc aaaaaaagga atatggtgta agcaccctgg
    20101 atattgcaaa aagattgctg gataaaggtt ttcatgctcc aactgtctat tttccgttga
    20161 ttgtgccaga agctatgatg attgaaccta cagaaacgga aagtaaggat tctctggatg
    20221 cttttgccag tgcaatgata gaaattgcgc aggaatgtaa agaaaatcca gaattattac
    20281 atagtgctcc actaactact cctgtgagaa gagtggatga tgtgcgagcg gtaaaaatgc
    20341 ttgatacctc tttcccactt gaggattgag aggtgaaacc aaagaatatc aggagatatt
    20401 atgaaaaaga tacttttact catagtcctg tgcttaggac tactttcact cagttttgcc
    20461 gctgaagtaa aaattggcgt ggtcaaaatg gaccgcatta ttcaggaaag caatgaactt
    20521 gctgaaataa gccgtatcta caaactggat atgcaaaact ggaaaaatca actgaacgag
    20581 ctggatgctc aaattaaaca aatggaacag gaatttgaaa tagctaaact gactaaaaca
    20641 gaagcagcta aaagagaagc tcaagccaag attgatgaaa aaaaggctga ggcaggacgt
    20701 cttttagaag aatattttgg tgaaggtgga aaatctgaac aacgttatat ggaacttctt
    20761 tcccccatca acactaaaat taataatata attaaaaaaa ttgctactga tgaaggatat
    20821 accatcatat ttgacgatag tatgggaact gtgctttatt ctgctccttc tattgatata
    20881 actgacctgg tcttattgga acttaataaa gatactgtta aacctactcc cgataacaaa
    20941 actaatacca ctggaactca aactcctaaa ccagaagata cttttaaacc aggtgaattc
    21001 atacctaaga aatgaaaaaa tttaacaaaa ctatcacacc agaagatatt gtagctgttt
    21061 gtggtggtga atatcatggt gaacagaata ttactctaca aagtgtagct gatcctgatg
    21121 aagcagattc cagttgtgtg attttctggg agcaagaaaa atatctggat gccgtaaaaa
    21181 aatctcctgc tgttctcatt ttttgccatc ccgacaaagc taataatctt cccaatagaa
    21241 acttaattct tcatcctcat ccctattttg cctttctgcg tttagtagat tggtggatag
    21301 aacaggatgc tgaaaaacct attccagaaa ttcatcctac tgctatcatt gattcttctg
    21361 ccatcatagg agatggggtc tatattggtc cttattgtat catcggcaaa aattgtcaga
    21421 ttggtaatga tagtataatt gaagctcact gttttattag taataatgtc tctatcggta
    21481 aaaatactca cctatatcct gatgttaata tctatgcaga taccaggatt ggtaatggag
    21541 tcattataca tagcggaacc gtcattggtg cagatggttt tggctttata ctgatggatg
    21601 gggttcaaaa taaaatcccc caggttggga atgtggtcat agaggatttt gtagaaattc
    21661 aggctaattc tgccgtagat cgtggaactc ttagctcaac ctttattgga cagggcacta
    21721 aaattgataa ctttgtccag ataggtcata attgtcatat tggcaaacat tgcattcttt
    21781 gtgctcaagt aggtttagcc ggaagcacta ttttaggaga ttatgtatat ctggcagggc
    21841 aagtgggagt ggcaggacat ctcaagattg gagatagagc aatggtcggt gctcaatcag
    21901 gcatcactaa tgatattcca gcggatgctc gttattgggg aagtcctgct atggaagcag
    21961 atagatttaa gcggattctg gtcagtcaaa aacatctacc agaaatttat cgcttttatt
    22021 tgaaacaaaa gaaaaaagag gaagaagaaa cttgaatatg acagagtata agaatacgat
    22081 taggactcca gtttcctaca ccggaattgg actgcataca ggagaaattt ccactattac
    22141 ttttaaacca gcaagaaaag atgaaggtat cgtctttatt aggacggatt tgcccgataa
    22201 acctgaaatt cctgctgata ttgaccatgt agtggatatt tcaagaggca ctactatcgg
    22261 ggttaaagat gccaccgttg ctactattga acatgtattg gctgctataa agggattatc
    22321 catagataat atcaggattg aaattgatgg acccgaagca cctgttgcag atggttctgc
    22381 tattgtgttc ttaaatcttt taaagaaagc tggtctggtg caacaggatg cggaaagagt
    22441 ttactttgaa tttgatgaac ctataagtta ttctgctcca gaagaaaatg tagatattgt
    22501 aattgtccct tctaatgaac ttaaggtcac ctttatgatt gattataaac atccttatct
    22561 gggaactcaa tatacctggt tacctgatat taaacatttt gaaaaagatt ttgctgctgc
    22621 ccggaccttc tgttttataa atgaagtcct tgaattgaag gagaagggat tgattaaggg
    22681 tggttctttg gataatgcac tggttatagc tgacccggat atgacagaag aagagctgca
    22741 acacattcaa gaagtcttcg gttatcatga aaaagttacc atctcaccac aaggtatcct
    22801 gaacaatcat cctttgcgtt attacaatga atttgtacgc cataaagttg tagacctaat
    22861 tggagacata gctcttctgg gagtgcccat aaaaggtcat attttagccg cccggagtgg
    22921 tcataagacc aatgttgaac tggtgaaaaa gctccgtcag atgcaattaa ctcaggaact
    22981 taagaaacaa tatcaaaagg ataactccaa ggatgttatc tttgatgcga atgccataat
    23041 gcgaataata cctcatcgtt atccattctt attggtagat aagattattg atttcaaacc
    23101 aaatgaatct atcgttggta ttaaaaatgt aaccataaat gaaccttttt tccagggaca
    23161 ttttcctgga catcctataa tgccaggggt tcttatcata gaaggtatgg cacagactgg
    23221 aggaattatc ctacttaatc agatggatga ccctaccaaa tatgttgcct attttgcctc
    23281 tattgataat gtaaaattcc gtaaaccagt tatgcctggt gatacgcttc gttatgaaat
    23341 gagagttatt tctttaaaac caaatctttc cagaatgcgt ggcgaagctt tcgtaggtca
    23401 cgataaagtg gcagaaggcg attttatggc aatcctgcaa aagaagtaat tatgaccaga
    23461 attcatccta ccgctatcat agaaaaaggt gccattattg gcgaagattg tgaaatcgga
    23521 ccctattgtc atattggacc agaagtcatc ttgggagatc ataatgtcct cagttccagt
    23581 gtggttatta tgggcaaatc agaaattggt aatgagaatc atatcttttc ttatgctgtt
    23641 attggtacag atcctcagga cttaaaatat cgtggcgagc ctacaggact gaaaatagga
    23701 gaccgtaata ttatccgcga atttgtcact ataaatcgtt ccaatcaaga aggagaattt
    23761 actatagtcg gaaatgacaa cctattgatg gagtatgtac atattgcaca taattgtcat
    23821 atcggttctc attgtgtggt ggcaaacact gttcaaatgg caggacatat aattgttcag
    23881 gattatgcca ctattggagg cttaaccgct atccatcaat tcgtccatat aggaacttat
    23941 gctttcgttg gtggtgcttc tgcagtcaaa aaagatatcg caccttacac cagaggacaa
    24001 ggtaatcctt acaaaactgt tggcttaaac accgtgggtt tgacccggag aggcttttct
    24061 aacgaagccc aaaatgccat aatgaaggta tataagctct tttattctga aggtttgaat
    24121 acttctcaag ctttagcaga agtagaaacc tggagcgatt taactcctga acaaaaagtt
    24181 tttgttgaat ttatcagaga gtgcgaaaga ggtctttccc gataaacttt gccttcctaa
    24241 aatacataaa attctccttt aagtggtgtc tatcagtgga caaaacttta gtgtaggaca
    24301 ttaaaatatt ttaggtgagc ggaatcagct tttttgtcag aaagaactct tccatttaat
    24361 ccagcataaa tagattcttg gtgaatattt tctaccagct aaatcctttt tattccattt
    24421 aattagcaaa gagtaatcta tatataatta ctctatttca cttgaatcaa ttatttttct
    24481 gcctttccct gataattaaa gctatcttat tgattaagaa caaaataaaa taagtccaaa
    24541 tagactttat accctttatt ttatctggcg ggaggtgaaa acttcttttc tcatttcatg
    24601 aaccatctct atcccttaac tcttgatgtc taatttatac ccagtttatg tccagcttat
    24661 gtccagctta tgtccaactc atgtccgaaa tcggtcataa gctggacata actaaaatat
    24721 gaatgggaga tataaggctg acggcgagtg aagttattta tagctaaaaa gcaagatatt
    24781 aagactaaat tctataaaag acaaccagaa ttgaaaataa tttagaaaag ggagagtaga
    24841 tttaattggg taaaaaataa cctgctgagt ttataaattt acagggactc agcaggttgc
    24901 atcttactat tatctatagt tttaagaact tttcgctagt agctgtaacc ttaaagaatt
    24961 gcatattacc agggttggtt atgatataat ttaaactggt agttgaggta attaaagtat
    25021 agtttccgta aggatcagaa gcaccataaa ttttataaga ggtagctccc tgcactgcat
    25081 cccaggaaag ttttatttga ttattcactt tttcaattct taaagaggga ggttctaagg
    25141 cttcttgtgc ttgggtagta aaatgccaaa tcataaaatt atcggaattg ggatcaggag
    25201 tatccataat ttcattaaca ggtaaaacta cccaatacca ggtattacca taacccaggt
    25261 taactccagg agctgcagaa aggttaaaac tggtttcggt agtttaaaaa taataggaat
    25321 caaataaagt ttcaggatct ggagctacat acactccata ataagcagcc attgaaccat
    25381 aaggtgatgg aacccaagtc aagattacat tattgggatc aagtcctacg ctattatccg
    25441 gaggagaaat taaagtcaca tgttcgggca cttctaattg agccaggata acattatttt
    25501 tacctactac aacaagcttc cctccaccag tactgatatt agttagctga gtaggattaa
    25561 aggttaaagg aggaaaaccc tcttcttgcc aggttgctcc attatcaaaa gatttttata
    25621 aagcccagct attgccttgt tgactgggat tggagctata accggctgcc caaaatacac
    25681 cattatgatc acaggttaca ctcatcatat gcccaatagc ttgttccgtt gccgctaacc
    25741 agcttgcagc tgtgtttcca cctgttgtgg tataataaat ttcaccgcca tctttaacta
    25801 aaacgccatt attggcatca cgaaaataaa tatcacgaat attagcattt gcacccactg
    25861 taatggaagt ccaggtgaca cctccattat ttgttatata aagtactcca ccgctacttc
    25921 cagcgtaacc attcaattca tttttccagt aagtgcaata aagattagca gaagtactaa
    25981 tattatctcc tacagtaaac caggtttcac caccatcagt agtcttggtc accttaccac
    26041 ctaagccaac aatatatcca acttggtcat taacaaagaa gattttgtaa aaagctttag
    26101 ctgaagagac tcccggaatt gtaaaaactg accagttgtc accaccgttt acagtgtgat
    26161 aaatattacc actggtcttc ccggtaaccc aaccctcatt tacattttta aaatagcagg
    26221 tataaaagag actggtagta cctgaaatat ttttctttat taaggtgtga ccaccatcca
    26281 ttgttcttaa aattgttcct ttatctccta ctatatacca gttattagcg tccagaacat
    26341 ctgcttgcca aagatcaata gcattaggaa ccagatcttc ccaggttatt cctccatcag
    26401 tagataacat caaagttcct tgccagccac aagcaagtaa acttccatca ttacggaatt
    26461 ttattccttc aatatagacg gtggcattat gattgagaag ttgccaagta gcccaaccat
    26521 ctgtagtctt gataatggtg gcatttcttc ctacggcata acccacattc ccttccatta
    26581 ctacatcacg cattgatgtg ccggtacctc cggctccata atagtgagtc caatttacac
    26641 cattatcatt gctgtaactt atgcaaccct tatctccaca tagcaccact ttattcccaa
    26701 attgacgaac cttattaaaa gaaggattgt cggttaccgt aagattaatt aaattccaag
    26761 tttgaccaaa attagtggtc cttaaaatct tggattgact gggagtccct ttattcagag
    26821 caacaaaacc aactcctgat tcattcatag atataccata acaagctaca gtggttacac
    26881 cttgtgcttg aaggctccag gtgactccaa aatcaggtga ataggcgact ttcccttcat
    26941 aaccacaaat ccaaattcta ccatcaggaa aatataccgc tccgtacatt ctggtagtac
    27001 ctataatatt ttgagcactt tgaggccaag tccagctggc ccctccatca ttagtgagag
    27061 caactaaacc atcgtctcca accaaaatgc cttgca
//
LOCUS       DAORZF010000020        26815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_1543_length_26815_cov_379.480717, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000020 DAORZF010000000
VERSION     DAORZF010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 26815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26815
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1543_length_26815_cov_379.480717"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1681)
                     /locus_tag="PK015_03630"
     CDS             complement(<1..1681)
                     /locus_tag="PK015_03630"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOL33594.1"
                     /translation="MDFYWKNSLFECLYYPDELGFTSGTITSLAFYNQFADSPANGAT
                     KIWLGSTNLADLSAGWIPSTQLTLVFDGIVTYPAGTNTITIPLQTPYMHTPGNLVMMV
                     QRPMDTQYYSSSDYFLAQTVGSNRARKAQSDSEAFDPANPPASATVSGQFPKTTIFYT
                     SQALVNDLGCLSISGNTTPSVGAATPYTITVKNNGTAVQSNYTVKLMKEGGVLLGSVP
                     GTTINSLQTLNFTIPWTPTETGSTYIYGEVEMTGDEIATNNQTPHLDIIVQAAGVVSV
                     TVGTGNSTGRMPMDFYYYNSLFETIYLASELNIGGLLTDIQFYNNFSSNLTNKPTNIW
                     VGETTQTDLSAGWIPSTQLTSVFSGNVNYPSGQNNILIHLTTPYAYGGGNLVVMVERP
                     MDTTWYSSSDVFKTQTGTVANRTRNAYSDSMDYDPANPPTVTPTAMFPMTTFMFITSG
                     LGSINGTVYTTGNVPLPGATVTVAGTSLSYTTGADGTFTFPYVTQGVHQVTATKHGYN
                     EVTNTVTVVENQTVTTNFTLTPLPQVTVSGRIVGSDQPTVGLANATISLTGY"
     gene            complement(1806..2729)
                     /locus_tag="PK015_03635"
     CDS             complement(1806..2729)
                     /locus_tag="PK015_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33595.1"
                     /translation="MKRLTLLLVMIITVCLAFAGIDEYYTFNATTGTYTPITGTDAAI
                     SSDDIISSAIPIGFTFPYGDYTYTEVIISSNGWIGLGTSQTSSNLSNQLASTTYVPVI
                     APLWDDCSLAAGTCEYLLSGTAPNRIFTIQYSNLKWNYYSTAMFNLQVRLYESGKIDF
                     VYGSTDGTPNSPSASIGINMLPGGSGWFYSVTPGTPATASTTTENNAVAVWPGEGTVY
                     EFNPVVAVPNDLAALSVTGNTTPTAGQSYDYIVTVRNRGSNPQSTYQVKLLLDTQEVG
                     SVNGTTIQPGEILTYTIPLDTHYSRSWSPLW"
     gene            2982..3758
                     /locus_tag="PK015_03640"
     CDS             2982..3758
                     /locus_tag="PK015_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOL33596.1"
                     /translation="MELNLWSWNVNGLRAAINKGFIETILREQPDWLGLQETKLQEHQ
                     IPSELGVLSNYYMYWSHSERKGYSGVAVFTKREPRIILYGFGVPEFDCEGRIIRCFYD
                     SFIIYNIYFPNGQMSDDRLNYKLRFYDKCLEVMEEDRKKSPMVIVTGDFNTAHKEIDL
                     ANPKENEKTSGFLPIERAWLDKIVAIGWIDTFRMFHPEPQRYSWWSYRTKARPRNVGW
                     RIDYFFVNEEAKDRIIDADIRDDIMGSDHCPVTLRISVPS"
     gene            complement(4570..5019)
                     /locus_tag="PK015_03645"
     CDS             complement(4570..5019)
                     /locus_tag="PK015_03645"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HOL33597.1"
                     /translation="MKKIVITIIALVCAGMVLGETTKNPNKAALYSAIFPGGGQLYNH
                     AWWKAGAVVGVQSYLISAAIYNKDKQEDYKTLAENTTDQFQQQIYQSKSKTYRDKFNN
                     DLWWIGITAGLSILDAYVDAHLYNFESEKEKIHLHFSENEIVLQYNF"
     gene            5219..7321
                     /gene="fusA"
                     /locus_tag="PK015_03650"
     CDS             5219..7321
                     /gene="fusA"
                     /locus_tag="PK015_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOL33598.1"
                     /translation="MESDSNLSKIRNIGIIAHIDAGKTTTTERILYYSGFLHKMGEVH
                     DGNTFTDWMEQERERGITINSATVTCVWKDKQINIIDTPGHVDFTAEVERSLRILDGA
                     VGIFCAVAGVQPQSETVWHQADRYKVPRLAYVNKMDRLGADYENVISMIHERLTPLAK
                     PINIPIGKEDEFEGVIDLITMKSYFFDPLTLGAEIHQEEIPAHLQEYAHKQREILLES
                     IAEFDDELLTAYIEGEQIKEEWIRRAIRKGTIENKFLPVLCGSSLKNRGVQLLLDAIC
                     DFLPSPLDVPPCKGINPKTKEIITLAPDPKAPFVGLAFKVQIDKYLGKLVYVRVYSGT
                     LKKGSSFLNQTNGKRERASRILQMMSNRKHDLNALHAGDIGAISGCRFLSTGDTITDG
                     AIDIILERMHFPESVISVAIEPRTKADQEALQNALTLLEDEDPTFNVRIDPETGQTLI
                     SGMGELHLEIIVDRLLREFNVAVNVGPPQVSYKETITKTVECEETFMRELNGKSNYAK
                     VRFRLSPLSQEELDRDVKNIFINNISPEKIPPEYYKPIEEAAMNALNDGPLISGTVER
                     VKIELIDGDYDPDNSNELAFRIATSIAISKGLREAEPVLMEPIMLLTVISPDDFVGDI
                     IADINTRRGHIFALRKHNEFQQEILAEVPLSELFGYATRIRSLSQGRAIYTLEFRKYE
                     IVPPQVQSSVLKRIRGYA"
     gene            7391..7699
                     /locus_tag="PK015_03655"
     CDS             7391..7699
                     /locus_tag="PK015_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOL33599.1"
                     /translation="MDIDLKVSENRALLSVRGSLNSDNAPQLQEKLNEVLESDIKYLD
                     IDFFECDNISSTAIGKLLLFYKDFISKGGEMEVIRCSTPIYELFNMLKLNQLFTVNLG
                     "
     gene            7696..8616
                     /locus_tag="PK015_03660"
     CDS             7696..8616
                     /locus_tag="PK015_03660"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOL33600.1"
                     /translation="MTVELNRILVVDDNPEILNDLATLLTIHQYQVDTTTSGCDAIRR
                     LRKVCYDLVICDIEIPDINGLDFLEKLRQHNWTQEVIIITGYLEQDYFIRAIRLGAAD
                     FISKPIDGQQLLNSIEAVKKKSLLKQNQMVPLEDFEEAQLSYVIDPTKFSQITINQIM
                     NPFLSQYLDLSQDTLNELLICADEMLTNAYFHGILELTKEERTQEFTQLKKIISQKLT
                     EPAIASRRIRFTISLNKEKNSIRITVEDDGKGFDYKNWLQQLAKPISINLDCYGRGLT
                     MLYHLSDSLVFSKGGRKVEIIRKLPSRNAE"
     gene            8613..9416
                     /gene="prmC"
                     /locus_tag="PK015_03665"
     CDS             8613..9416
                     /gene="prmC"
                     /locus_tag="PK015_03665"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOL33601.1"
                     /translation="MTVGALLNKHRKEAEYHDISLLLCELLDCPFLTLSLRKEEELSD
                     RLLENFFTNYARLRNKEPVQYIIGKAWFYGLEFSVNPEVLIPRPETEGLIELALQFIP
                     ENAKVLDIGTGSGAIAVTLKTLRPDLTLVATDISASALKIAKQNADYNNCAISFYQTD
                     LYPETEDSFDLIISNPPYISPSDYAKLDTRIKEYEPKLALWAEENGLIFYRRILKEAK
                     SKLKPEGLIFFEHGDEQRQNIIHLAETYGWETILIKDDLAGYPRYLGFK"
     gene            9464..10711
                     /gene="murA"
                     /locus_tag="PK015_03670"
     CDS             9464..10711
                     /gene="murA"
                     /locus_tag="PK015_03670"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOL33602.1"
                     /translation="MEQFIIEGNRNLSGTIPVSGAKNAILPAMAACLLAPGTSTLHNV
                     PHLIDLKTMSLLLRVLGAKVEMENDVLSIDASHIDSYEAPYELVNKMRASIYVLGPLL
                     ARLGIAKVSFPGGCAIGTRPVDLHLMAMEALGAHITVEHGYINAKADKLVGADIYFPK
                     SSVGATINALMAAVLAKGVTSLFNAAMEPEIDATIDFLNLMGAQISGKGTTTLIIEGV
                     EKLHPAEMEMIPDRIEAGTFLIAGALSTNPVTVTNCNPNHLTLPMEKLRASGCELEVE
                     NCSITVYPPEIIQPVDIITLPYPGFPTDLQAQFTVLMCLADGISFIEDTIFPDRFAHV
                     AELNRLQADIRVEQNIARVKGVKKLSGAEVMATDLRASAALVLAGIAAEGKTVISRIY
                     HIDRGYDHIEKKLNAIGAKITRS"
     gene            11056..12774
                     /gene="recJ"
                     /locus_tag="PK015_03675"
     CDS             11056..12774
                     /gene="recJ"
                     /locus_tag="PK015_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOL33603.1"
                     /translation="MEKEWKMPPELSEEDKQKIKDISVSLKCPKIVAELLYRKGLITI
                     EEIDAFLHPRLDGLFDPFLFPDMEKAVQRIIRAIDKGEKITIYGDYDVDGTTATTLLY
                     LGLNRIGANIDYYIPHRMIDGYGLSLSSLDQLKENGTALIISVDCGVNALEEIEAINK
                     RGMEIIITDHHNPKEELPEAVAIINPKLPGCTYPFPYLAGVGVAYKLLIAIYQARGLE
                     SLENTLKYMDLVAVGTIADIVPLIGENRIFAHIGLQHLIEKKNLGLNALVQIAGLKSK
                     VLDSSDIVFGLAPRINAAGRMGSASLAVELLISRDIEQSKELAESIERLNSLRQQEDQ
                     KTFQEACEIIEKKYSKLETVPCIVVSSDDWHPGVIGIVASKLAEKYYRPVLMISFKEG
                     WGCGSGRSVADFDLFNALKAVEHNLPSYGGHKYAVGFTILQEYLDRFENELSRYIADH
                     LQLEQIKPSLEIDHEIELYDISEFLLDWIEQFAPFGPENSRPVMLTRNVTIAGYPYTV
                     GRNHLKLKVVKDGVQLDLIGYNLGDYLPLLKKNMEIDIVYTLEYNRFGDKINIQGKLK
                     DIHWSK"
     gene            12785..13603
                     /locus_tag="PK015_03680"
     CDS             12785..13603
                     /locus_tag="PK015_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33604.1"
                     /translation="MKRCSGIFIILLSLLFLGNCADNSFIARRDIDHFEFIRAIPVGI
                     EYDKAIYSAPTNSVFAMQRASQEIHIYRDGKRINSIGGLGFDRTNFQRLNDIGIDTDG
                     GLLALDKVTKVLRKFSPEGLFISEIKFDTLNQPELFCIGKDGTIFVYDSTSSEIISIS
                     PLDTKELYRFGRFEIDQPMYIDCNNDFLFAYNSKKNVTYVFYLYGQYKETLTGQFVYD
                     LINHPISVNNIYPLIGRQTPPKLMTTNAQMLTLLYPQEICLVKIVYKRGIDATP"
     assembly_gap    13696..13795
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13796..15310
                     /gene="lnt"
                     /locus_tag="PK015_03685"
     CDS             <13796..15310
                     /gene="lnt"
                     /locus_tag="PK015_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOL33605.1"
                     /translation="LSRLPFHLGWMVFFAWVPLLYVFETGKAKSGDLLRMGFIMSIVY
                     MSIVFYWVTLVTIPGLIGMIAFYGFVYYILFYAINRIFLVLPKWRYLGFISIMITFEY
                     IQNFGETRFPWFNLAYSLADYTHLIQFADIGGVVGLSALILLINILIHLLLPTSQKFL
                     ENRGRNHYVNTSPRKRQITSVVAIVIILISWWGYGEYCLKNLPLEKHEAGIFVVQPSI
                     DARERWKPEQYDKSLELFRQLTLEAVADSAKLVIWPEGAMIGDLASDYAAQRDLQRII
                     DEAQVDIFTGFQHYETDVNHPRGERSYNAASLFQPDNFHNELYFKNILVPVAERMLWM
                     DIFPFLWKLDLGQANWEFGTKLAYYESGGYRFSPSICYELAFPEIFHRMAIPRDSLSG
                     EFNKCDFLVNITNDGWFGTSYGPWLHAMMARFRAIENRIQIYRSANTGISLIVDPKGR
                     ILARTKLNEVCVITAPLYTTPKIPVIRRIYKYPFIFVGIALLLALIARIKKPGG"
     gene            15315..15692
                     /locus_tag="PK015_03690"
     CDS             15315..15692
                     /locus_tag="PK015_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOL33606.1"
                     /translation="MKKYYYTIGEVSNLLGVKPYVIRYWESEFSFLKPRKESGRIRKY
                     SEENIELLRKIQDLLYNQHYTIEGARQKLKQELRNKSSHEPVPVSPQNNAINAELQEN
                     LAALRQNLKEICNLCHQLRSRKI"
     gene            15689..16543
                     /locus_tag="PK015_03695"
     CDS             15689..16543
                     /locus_tag="PK015_03695"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HOL33607.1"
                     /translation="MNFQDLILTLQNYWSEQGCNLMQPYDMEMGAGTFHPSTFFGALG
                     GKPVSVAYTQPCRRPKDGRYGENPNRLQHYFQFQVIIKPGPDNIQDIYLKSLEAIGIE
                     IAKHDIRFVEDDWESPTLGAWGLGWEVWLDGMEISQFTYFQQVAGFDVFPISVELTYG
                     LERLAMYIQDVDDYRKLRWNHHTLYGELYFPREVEYSEYNFNAADTSLLFDLFADYEK
                     ECRELLAKNLVFPAYDNLLHCSHTFNLLEARGVISVTERAAYIARIRALAKEVATAYL
                     AKYDDKTR"
     gene            16555..17337
                     /locus_tag="PK015_03700"
     CDS             16555..17337
                     /locus_tag="PK015_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HOL33608.1"
                     /translation="MIYSEWHFGNDNIPLLATAIHNGHHFPLELVGFCGVDEKDRLRE
                     EDPYTSEFASLSPNYVVNYTSRFVVDLNRSLEKAVYLKPEDCWGLNPWLKPLPEEYLN
                     KLYEDYDAWYSLLRYQIERMLKTHPFLIILDLHSYNYLRNGPETEPEPNTPDVIIGRS
                     NLKSDYYPLIDSLREIMGNNTLQNRKLDCQLDVKFTGGNLSQWLNAHYQDKLICLAIE
                     FKKIFMDEWTGELYPTTFTQLKTLFKESIASWLQNSFNIQLT"
     gene            complement(17347..19038)
                     /locus_tag="PK015_03705"
     CDS             complement(17347..19038)
                     /locus_tag="PK015_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HOL33609.1"
                     /translation="MKSKIILIILSFLFLASGIFAEDLYLDIMFTNDMHGGIDRSEAT
                     FINPDFPPRLGGGASAATLIKYARSLSNAQRSTLLFDIGDFFQGRPVGTITKGKAIIE
                     YMNAIGYDAMTLGNHEFDITEDELKETLALAKFPVVSCNIIDKRTGDFPPYVVPYIIV
                     DRLGVRIAIIGLTTTDTEKMSFPENIKNLDFLDEKGSLTKYVKIVREEEQADIVIVLG
                     HAGLPYDNIETYLTRYDNKGNPLFTERYAVWGYDAQELAREVPGIDLFVGGHIHKGIP
                     SPWIDPYTHTMVIQGYAYGSNLGWMTLTIDSETKSVSGYKLPALTDGMMITLFEEEFI
                     PDAEISPIIEEQVAKAEEGMDEVVGYAAVNLTRTNYDAQSLVGNMIVDAMRNEVKADF
                     AFLNLGGVRADIKAGPVTYRDIFEVMPFDNMLVSFTCDGEFLRRIIETRVEGSRHGLI
                     VSGVNVVYSKKRPNYDRVTSLKVGGEPWDPNKIYTCVTTDFLMEGNAGLTLLTQVPKE
                     NIIYHQINLRDAIVHYFQQHSPVHTKIDDRWKRDDNAKPTPEMLRAKSQATKKEE"
     gene            complement(19044..20435)
                     /locus_tag="PK015_03710"
     CDS             complement(19044..20435)
                     /locus_tag="PK015_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33610.1"
                     /translation="MIKKLALSVILSIIIIAGLSAWSITDDYFGNRYGMLDARSYAMG
                     STGIFNELRPSAITLNPANLTLINRPAGLEGSFFLNRNEDNRAVPLYNSFDNYIDDSV
                     YASNLNFYDDYATAGFVSFKPWKLRIGIGAYYQPLLSFDGDYKEEIRNNRNTDNDNYP
                     EKIAVNTIENAGVLNQASGVLSLGFALGENSELNLGFDFGKLWGNTKYQKDILWTQWA
                     IDTVGEGVLPDSIYKENVDWDGYRYKTGVSARINKRLGIGATYTFKTTLDRTIDWVKT
                     YGGVEVSSTTNAKEDYILPSEFRLGINYQPRNIMRTWFNLEGEYVRFSEIGDQYDDVI
                     NFYAGVEHNIKYSLPFRFGFQATNSYLRTVEADNSIIVKRIITPMITGGSSIPLTDKL
                     HLDLGFGYSWREYEALDLFRDSYYDDRHYTGENSYQLWPNQHIALTDRGWENPDKVRE
                     SNISLSTSLSYTW"
     gene            complement(20448..23069)
                     /locus_tag="PK015_03715"
     CDS             complement(20448..23069)
                     /locus_tag="PK015_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOL33611.1"
                     /translation="MKKFIVFFFVLLCVMPLVAKIDLNTATFEELMQLPITEKQARDI
                     LEYREYVSIFKDVYQLREIPSIDQKTLLNLKDLVVVSIYQEEDEALARRQQIQDLLER
                     LDSNEGSSEGMSDVWQDYLMTPHNVNKMHFDDFISLPNVSSVDAVSILKRVALGDTIA
                     DMRDLRNTTGLSYYGYTNLRSYVYYKEPPVKNRFFWDAELQYYTRYLEEGASDMYHEP
                     FLRNDYGNSSIVVPQHKNLSYWGYFNMDKIDPDVMVKLRARYGNNYKIGWMHYKAKGE
                     TSLSDKTTKEILADSKFYAGYENNELPLLDNTRLKIYLGNYRVTYGEGLVMENTDFYS
                     ARKTGYGFSKRILGITPDLSRTQEYALRGAAFELTHPYAGLSFWFSQDDKDAIVYVDE
                     YGNTVKEEGKDKIFSYVVPTIRFDNDELREAEAMFNQELQSGTPYATNYINLAYRKDA
                     LKEKLWGTHLQVNPFIGTKIGFTTYTALYDDAYFMVPDYNDLKTLLVRDSYYYPKFKM
                     MDAEIANLYSTRTDTYSRDYRRVIGFDGHTTLGNISIEGEYAELSVDGSDLKLGDDPK
                     AYLISAHTQFDNLYFISLYRHYDVDFDNPYSNAYSEHQRFEDTILEKNIYALTNPTLS
                     DLYLNCSQAQPEEGIYFETRYKFDRFFTINRSYLDIYERLTDGRRSVRFQTELEFRPI
                     YALGLRLRYKNQVNRYDDYAERGVSKTDEYTVSVRTFLSNRDFLEFEYRYNTVWGPPY
                     TSLTYPAEPGNNTMAAAQTLMVGDYIGVNYTHNFNTNLKLQGSFIYWFGHGISHWDWE
                     DMEIDFMGEKGAKFWVALHSRISQNMYLTLKYRNKTYQTQELSIREYNVPVQGENLFQ
                     RVEHKENTIRLSLEYRY"
     gene            complement(23256..24044)
                     /locus_tag="PK015_03720"
     CDS             complement(23256..24044)
                     /locus_tag="PK015_03720"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOL33612.1"
                     /translation="MKKSYIFVFISLAMLIITSLSAEVTIPYLSEYLYSYPSTIYKGA
                     TAPFNWTKDDYLKAGGLLLTGGMLYLEDEHLRDLYQRNRIESVDDIFTVFEVLGNAKY
                     MFPALGVMTLSGYCLKSDKTVETGLLSIKSAVLAQGLTLSLKIITQRPRPETEEGKEF
                     WSTGSFSLHNDSFPSAHSTLVWSLAPIFAEQYKENKWVPPLSYTIAILTSCSRLNDDK
                     HWSSDVFIGSIIGYFTAQLVLKDTPRLTFNFSPDLSGIGFCYEF"
     gene            24498..24845
                     /locus_tag="PK015_03725"
     CDS             24498..24845
                     /locus_tag="PK015_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOL33613.1"
                     /translation="MKLSDIVKILDAEVLTPEVNLDIEVPCAFASDLISDILMCTKEP
                     TLLLTGLTNQQVIRLADMIDIIGIVFVRGKKPPAEVIEMAKERNLPLICTKNTMYRSS
                     GLLFNAGLRSCRI"
     gene            24817..25350
                     /locus_tag="PK015_03730"
     CDS             24817..25350
                     /locus_tag="PK015_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOL33614.1"
                     /translation="MLDYAVAESEMAEYWYIRPGYEKAVQKHFENQQEPEVSLESLIR
                     EKDFNEAGKGSAEVKNVLKRLGIDPAILRRIAVASYEAEINVAAHSLGGAMLSHIYQD
                     RIHMIFMDNGPGIADIEQAMMPGFSTADEMVRELGFGAGLGLPNIQKNVDALHIVSEA
                     GDSTMLEFLIFFNDNGQ"
     gene            25340..26650
                     /locus_tag="PK015_03735"
     CDS             25340..26650
                     /locus_tag="PK015_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOL33615.1"
                     /translation="MDNKEYFHAVHIIEDNCTGCTSCVRVCATEAIRVRNRKAHIDPA
                     RCVDCGNCIVACKFHAIIPQSDPLELINKFKYKVAIVSTSYFGQFSEDISYEVAKQAI
                     YELGFDQVEEEAMVTDFMIQMIRDYVRKHRDKRPILSSNCPTVVRLIQLKFPSLLPNI
                     FQIEAPMSILTRYLRDKIQKEKGLKEDEIGIFLIVPCPAHVTAVHQPEGAYKHLQDGA
                     FAISMIYGKVHEHIKRLQNQPTPDIVTYPKGLTWALSGVQADLVDCEDIRALSVNGID
                     NVIEILSRVEDHYLDQYDYIVLRSCTNGCVGGFFNVENPFVAMSRIKKMIKEGKDTPT
                     DLNELELLYKAGEFAVAPLSPRPIMELDKDIKTAIVKMKQLNEILTTLPGLNCCACGS
                     PSCYALAEDIVQGKATIDDCLVLLKRHTLENIQDDILKKNKSKE"
BASE COUNT         8529 a         4873 c         4989 g         8324 t
ORIGIN      
        1 cataaccagt taagctgatg gtggcattag ctaaaccaac cgtaggctga tcacttccta
       61 caatcctacc acttaccgtt acctgaggta gaggagtgag agtaaagtta gtagtaacag
      121 tctgattctc tactactgta acagtgttag taacttcatt ataaccatgt ttggtagcag
      181 taacctgatg tacaccctgg gtaacatagg ggaaggtaaa ggttccatca gcaccagtag
      241 tgtaggataa agaggttcca gccactgtta ccgtggcacc tggaagggga acattccctg
      301 tggtataaac ggttccatta atagagccga gtccacttgt tataaacata aatgtggtca
      361 ttgggaacat agcagtaggt gtaactgtag gaggattagc aggatcataa tccatactat
      421 cactataggc attgcgagtc ctatttgcta ctgttccagt ttgagttttg aatacatcag
      481 aggaactata ccaagttgta tccataggac gctcaaccat cactacaaga ttgcctccac
      541 cgtaggcata aggggtggtg aggtgaataa gaatattgtt ttgtccgctg ggatagttca
      601 catttccact aaagacagaa gttaattgcg tggaaggtat ccaaccagct gaaaggtcag
      661 tttgagtggt ctctcctacc caaatattag ttggcttatt ggttagatta cttgagaagt
      721 tattatagaa ttggatatcg gtaagcaacc cgccaatatt gagttctgaa gcaaggtaaa
      781 tcgtttcaaa cagagaatta taatagtaga aatccatagg catacgacca gtactatttc
      841 ctgttccaac agttacagag acaaccccag cagcttgtac tataatatca agatgaggag
      901 tctgattgtt agttgctatt tcatctcctg tcatttctac ttcaccatag atataggtag
      961 aaccagtttc agtgggagtc cagggaatag tgaaattcag agtctgaagc gaattgatag
     1021 tagtcccagg aacggaacca agaagtactc caccttcttt catcaatttc accgtatagt
     1081 tgctctggac agcagtacca ttgttcttta cagtaatggt ataaggtgta gctgcaccta
     1141 cactcggtgt agtattgcca ctaatactca gacaacctaa atcatttaca agtgcttgtg
     1201 acgtatagaa aatggtagtc ttagggaatt gaccagacac agttgcactt gctggaggat
     1261 tggcaggatc gaaggcttcg ctatcactct gggcttttcg tgctctatta gagcctaccg
     1321 tctgagctaa gaaataatcg gatgaagaat aatactgagt atccatcggt ctttggacca
     1381 tcatcaccag atttccagga gtatgcatat aaggagtctg taagggtata gtgatggtat
     1441 tagttcctgc tggataagta acaattccat caaaaaccag ggttaattga gtggatggta
     1501 tccaaccagc actcaaatct gctaaattag ttgaacctaa ccagatcttg gtggctccgt
     1561 ttgccggaga atcagcaaac tgattgtaga aagctaaaga agttatggtt ccactggtaa
     1621 aaccaagttc atcaggataa taaagacatt caaacagaga gtttttccag tagaaatcca
     1681 tcggcactcg agctgattga tctcctgcac ctatggttac tgcttgtact ccagcaggct
     1741 ggacagcaac attaagagga ggagtttgat cgttattagg attttgatct ccggctaaga
     1801 ctactttacc ataaaggact ccaggaccgg ctgtagtggg tgtccaaggg aatagtatag
     1861 gttaaaattt caccaggttg aatggtagtt ccatttaccg aaccaacttc ctgagtgtct
     1921 aataataatt taacttgata agttgattga gggttactac cacgattacg aacagttacg
     1981 atgtaatcat aactctgacc tgcagtagga gtggtgtttc cagtaacaga aagagctgct
     2041 aaatcattcg gaacagctac tacgggatta aactcataaa cagttccttc accgggccaa
     2101 acagcaacag cattattttc agtagtagta gaagcagtag ctggagttcc tggtgtcaca
     2161 ctatagaacc aacctgaacc tcctggaagc atattgattc caatagaggc agaaggacta
     2221 ttgggtgtcc catcggttga gccataaaca aaatcaatct taccactttc atataatcta
     2281 acctgtaaat tgaacatagc agttgaataa taattccatt tcaagttaga atactgaatg
     2341 gtaaagatgc gattgggagc tgtgcctgaa agcagatatt cacaagtacc tgctgccagg
     2401 ctgcagtcat cccatagggg tgcaatgacg ggaacatagg tagtagaagc taattgattg
     2461 gaaagattac tactcgtctg agaagtaccc aaaccaatcc atccatttga tgatattata
     2521 acctctgtat aagtgtagtc cccataagga aaggtaaatc caatgggaat agctgaggaa
     2581 ataatatcat cagaggaaat agctgcatct gttcccgtaa tgggagtgta ggtccctgtt
     2641 gtggcattga aggtatagta ttcatcaata cctgcaaaag ccagacatac ggtgattatc
     2701 atcaccagaa gtaaagtgag tcttttcata agttttctcc ttatcttatt tactgtatca
     2761 ttttacagtt caaaacatta aagtaataac aaaagaataa tattacttcg taatcttcaa
     2821 aatatccaca taaccaatat gtcaagtgaa aaaatttgat tttaccacaa cccttggtaa
     2881 ttatttatct ttatcaaggt attttctctt gacaaattat ctaaattcag attgtataat
     2941 taagttaaaa ttaattaatt ttaaacattt gaggtggagc tttggaactt aatctctggt
     3001 cttggaatgt taatggtctt cgcgcagcta taaataaagg ttttatagaa accattttaa
     3061 gggaacagcc agactggttg ggcttgcaag aaactaaatt acaagaacat caaattcctt
     3121 ctgaattagg agtattgtcc aattattata tgtactggtc acattctgag agaaagggat
     3181 attctggagt agctgtcttt actaagagag agcccagaat aattctatat ggatttggtg
     3241 ttcctgaatt tgattgtgag ggtagaataa tccgttgttt ttatgatagc tttatcatct
     3301 ataatattta tttccccaat ggtcaaatga gtgatgatag gctaaattat aaacttagat
     3361 tttacgataa gtgtctggaa gttatggaag aagaccgcaa aaaatcacct atggttattg
     3421 taactggaga ttttaataca gctcataaag aaatagacct ggcgaatcca aaggaaaatg
     3481 aaaaaaccag tggattcctt cctatagaaa gagcttggtt agataaaatt gtagcaattg
     3541 gctggattga tacattccgt atgttccatc cagaaccaca acgctattcc tggtggagtt
     3601 atagaacaaa agccaggccg agaaatgtag gctggcgtat tgattatttt ttcgttaatg
     3661 aggaagcaaa agaccgaatt atagatgcag atattcgtga tgatattatg ggttctgacc
     3721 actgtccagt gacattacgt atctctgtgc catcataatt atttaaaaga tataaaaaat
     3781 atagcttaga gttcccctac ctaagaataa ataatatatt acacttagct ttataattga
     3841 cataaaatat gatgaacgat actattaaat atataagacc atatcacacg caaatgagca
     3901 aaaaagaggt tataattatt ttaaataaat gagttacaaa atccgtttta aaagaaagtt
     3961 atagctgaga ttgaggtttg aaattcccct ttttagagga aaaaatagtt ataatcaaat
     4021 atattcccct tcagttaagc aatgtgttag aaactcaaaa aataaatata taaattctat
     4081 tcccaactcc gggaactctt gattattaaa tgtctccctt atacagcctt tgtattgaaa
     4141 aaattacaac tgatttatta aacttctatc gttttttatc aaattataag attcaaatcc
     4201 aattattatt caagcaatta gactcatatt tgtctcatat tttttcaatt tttgctttta
     4261 tcggtttaag cattaagtct cccttagctc tctcctgttt cttttttatc taaagtgcta
     4321 agagagtggc aagtaggagc ttaggctaaa tcagcgggta aaataagcaa aataaataaa
     4381 atataataga actattgatg atagaataat tacattaagg catacataac tataatgatt
     4441 atataatagt tataaaatca attgcaattt actgatttat tgtgtggtat tacaatgtaa
     4501 atagctgccc tacctattca ctattaatat taattatcgt taaagcaatt ggatagagga
     4561 tattataaac taaaaattat actgcagcac aatctcattt tcagaaaagt gtagatgaat
     4621 tttctccttt tcgctttcaa aattgtacag atgagcatca acataggcat ctaagatgga
     4681 taaccctgca gtaataccta tccaccagag gtcattatta aatttatcac ggtaggtttt
     4741 acttttggac tgataaattt gctgttgaaa ttgatcggta gtgttttctg ctaatgtttt
     4801 ataatcttcc tgtttatctt tattatagat agcggcacta atgagataac tttgcactcc
     4861 gaccactgcg cctgctttcc accaggcatg attataaagt tgccctcctc caggaaaaat
     4921 agcagaataa agtgcagctt tattaggatt tttagttgtc tctcccaaga ccatacctgc
     4981 acaaacaagg gcaattatcg tgattacaat ctttttcaaa tctctatcct cttttattct
     5041 tttatttatt actgtctttc tctgtcaatg attttttact cgtactcaaa ggtctggata
     5101 aaattctatg atggctttta attttttaat aagtaaaaaa ctttttaatg gacagaactg
     5161 cttaatctgt ttttttggaa aacaagtata attttggaga agaattaaac tatttattat
     5221 ggaatcagat agtaatttat caaaaatacg taatattggt atcatcgctc acattgatgc
     5281 gggaaagaca actaccactg aaagaatact ttattactca ggcttcttac acaagatggg
     5341 tgaagttcat gatggcaata catttacgga ttggatggaa caagaaaggg agcgtggaat
     5401 aacaattaat tctgcaactg tcacctgtgt ttggaaagat aaacaaatca atataataga
     5461 cacacctggt catgtagatt tcactgcaga agtggaacgc agtcttcgca ttctggatgg
     5521 agcggtagga atcttttgtg ctgtggcagg agttcaacct cagtctgaaa ctgtctggca
     5581 tcaagctgat cgttataaag tacctcgtct ggcatatgtg aacaaaatgg accgtcttgg
     5641 tgcggattat gaaaatgtaa tatcaatgat tcatgagcgt ctaacacctc tggcaaaacc
     5701 aataaatatt cctatcggca aagaagatga gtttgaagga gtgatagacc ttatcactat
     5761 gaaatcctat ttttttgatc cccttacctt aggagcagag attcatcaag aagagattcc
     5821 tgcacattta caagaatatg cccacaaaca acgagaaatc ttgctggaaa gcatagcaga
     5881 atttgatgat gagctgctta ctgcatatat agaaggggaa cagataaaag aagaatggat
     5941 tcgtcgtgcc attaggaaag gaaccattga gaacaaattc ttgcccgttc tttgtggttc
     6001 atccttaaaa aatagaggtg tacaacttct tttagatgct atctgtgatt ttctcccttc
     6061 tccattggat gttccaccct gtaaaggtat taatcctaaa acaaaagaaa ttataacttt
     6121 agcacctgac cccaaagccc cgtttgtagg tttagctttt aaagtacaga ttgataaata
     6181 tcttggtaaa ttggtctatg ttcgggttta ttctggcact ttgaaaaaag gttccagttt
     6241 tctaaatcaa accaatggaa aaagagaacg tgcttcacgc attttacaga tgatgagtaa
     6301 tcgtaaacat gaccttaatg cacttcatgc aggagatatc ggtgcaattt ctggttgccg
     6361 ttttctaagt actggagaca ctatcaccga tggtgctata gatattattc tggaaaggat
     6421 gcattttccg gaaagtgtga tctctgtagc tatagaaccc aggactaaag cagatcagga
     6481 agctctccag aatgccctaa ctttattgga agatgaagat ccaactttta atgtccgaat
     6541 tgatcccgag actggacaga ctcttatatc tggaatgggt gaattgcatc ttgaaatcat
     6601 tgtagatagg ttacttcgcg aatttaatgt ggcagttaat gtgggaccac ctcaagtgtc
     6661 ttataaagag acaataacaa aaactgtgga atgcgaagaa acttttatgc gggaattaaa
     6721 tggtaagagt aactatgcaa aagtgcgctt ccgtctctct cctcttagtc aagaggaatt
     6781 agatagggat gtgaaaaata tctttatcaa taacatctcc cctgaaaaaa tccctccaga
     6841 atattataaa cccattgaag aagcagctat gaatgcttta aatgatggac cactaatcag
     6901 tggcactgtt gaaagagtga agatagagct aatagatgga gattatgacc ctgataattc
     6961 caatgaatta gcctttcgta tagctacttc tatcgctatc agtaagggtt tacgagaagc
     7021 tgaaccggtg cttatggagc ctattatgct actcaccgta atcagtcctg acgattttgt
     7081 gggtgatatt attgctgata ttaatacaag acgaggtcac atttttgctt tacggaagca
     7141 caacgaattt caacaggaga tcttagcaga agttccgctg tcggaattat ttggatatgc
     7201 cacacgaatc cgatccctca gccagggcag agccatatac acacttgaat ttcgtaaata
     7261 tgaaatcgta ccacctcaag tacaaagttc agttttaaaa cggattcgtg gttatgcttg
     7321 atattaaact tgacagcttt aattaaaaat attatagtaa atataaataa ttaccttaaa
     7381 ggaggatatt atggatattg atttgaaagt ctctgaaaac cgtgctcttc taagtgttcg
     7441 tggtagcctt aatagtgata atgcaccaca actgcaagaa aaactgaacg aggttctgga
     7501 gtctgatatt aaatatttag atatagactt ctttgaatgt gataatatta gtagcacggc
     7561 aatcggcaaa ctactccttt tttataaaga ttttattagt aaaggtggag aaatggaagt
     7621 tattcgttgt tccactccaa tttatgaact atttaatatg ctgaaactca atcaactttt
     7681 tactgttaat ttaggatgac cgtagaactt aatcgtatcc tcgttgttga tgataatcca
     7741 gagattttaa atgaccttgc aactctactg acgattcatc aatatcaagt tgataccacc
     7801 acctctggtt gtgacgctat ccgaagatta cgaaaagtat gctatgattt ggttatttgt
     7861 gatattgaaa tacctgatat caatggtctt gattttttag aaaaacttcg ccagcataat
     7921 tggacgcaag aagttataat cataacagga tatttagaac aggattattt tattcgtgct
     7981 atacgcttag gtgcagctga ttttatcagc aaacctattg atggtcaaca gttattgaac
     8041 tccattgaag cggtaaagaa aaaatctctt ctcaaacaaa accagatggt acccttagaa
     8101 gattttgaag aagcccagct atcatatgtg atagacccta caaagttttc acagataact
     8161 attaatcaga taatgaatcc ttttttatct caatatctgg acctgtctca agatacctta
     8221 aatgagcttc taatctgtgc agatgaaatg ctaaccaatg cctatttcca tggtatatta
     8281 gaacttacaa aagaagaaag aactcaggaa tttacccaac tgaaaaagat tattagccaa
     8341 aaattgactg aacctgctat tgcctcccgt cgtattcgtt tcactatttc tctaaataaa
     8401 gaaaaaaata gcattcgcat aaccgttgaa gatgatggca agggatttga ttacaaaaac
     8461 tggttacaac aacttgctaa acctatttct ataaatctgg attgttatgg cagaggattg
     8521 actatgcttt atcatctttc tgattctttg gtattttcca aaggaggacg aaaagttgaa
     8581 ataattcgca aactcccctc caggaatgct gaatgacggt tggagcatta ctgaacaaac
     8641 accgaaaaga agcagagtat cacgatattt ctctgctgct ttgtgagttg ttggattgtc
     8701 cctttttaac tttatcttta agaaaagaag aagagctttc ggataggctg ctggaaaatt
     8761 tttttactaa ctacgctcgt cttagaaaca aagaacctgt gcaatatata attggtaaag
     8821 cctggttcta tggtttagaa ttttctgtaa accctgaagt gctaattcca cgtccagaaa
     8881 ccgagggtct aattgagctg gctttacaat ttatccctga aaatgccaaa gttttggata
     8941 taggcacagg aagcggtgct attgccgtta ctctgaaaac attaagacca gacctaacac
     9001 tcgttgctac cgatatttca gcttcggcac ttaaaattgc taagcaaaat gctgattaca
     9061 ataactgcgc aatttccttt tatcaaacag acctttatcc tgaaactgaa gacagttttg
     9121 acctcattat tagtaaccct ccttatataa gcccatctga ctatgcaaaa cttgataccc
     9181 gaataaaaga atacgaacca aaattggcac tatgggcaga agaaaatgga ctgatttttt
     9241 atcgtcgcat tctgaaagaa gctaaatcta aattaaaacc agagggatta attttctttg
     9301 aacatgggga tgaacaaaga caaaatatta tccatttagc tgaaacatat ggttgggaaa
     9361 ctatattgat aaaagatgat ttagctggat atccgcgtta tctcggcttc aaataattct
     9421 aatcccagat ttaatccgag caaatcagaa ataaggaatg attatggaac aatttattat
     9481 agagggaaat cgcaatctta gcgggaccat tcctgttagt ggagctaaaa atgccatcct
     9541 tcctgctatg gctgcttgtt tacttgcgcc tggaacctct actctacata atgtccctca
     9601 ccttatagat ttgaaaacaa tgtccctgct cctacgagta ttaggtgcaa aagtggagat
     9661 ggaaaatgat gttttgagca tagatgcttc tcatattgat tcttatgagg caccttatga
     9721 gctggtcaat aagatgcgag cttctattta tgtgctgggt cctcttttag ccagattggg
     9781 cattgccaaa gtctctttcc ctggaggttg tgccataggt actcgtcctg tagaccttca
     9841 tcttatggca atggaagctc ttggtgctca tatcacagtt gaacatggct atataaatgc
     9901 taaagctgat aaacttgtag gagctgatat atatttccca aaatccagtg ttggagcaac
     9961 tattaatgcc ttgatggctg ctgttctggc taagggagtg accagtttat ttaatgctgc
    10021 tatggaacca gagattgatg ccactattga ttttttaaat ctgatgggag ctcaaatttc
    10081 aggtaagggc actaccaccc tgataattga gggagtagaa aaactccacc ctgcggaaat
    10141 ggaaatgata cctgaccgga ttgaagcagg aacttttctt atagcaggag ccctgagcac
    10201 taacccggtt acggttacaa actgtaatcc caatcactta actcttccca tggaaaaact
    10261 cagagcttct ggatgtgaat tggaagttga aaattgttct ataactgtat atccccctga
    10321 aattatccaa ccagttgata ttatcacatt accctatcca ggttttccta ccgaccttca
    10381 agctcagttc acggtgctga tgtgtttagc agatggcata agctttatag aagatactat
    10441 tttccctgat agattcgccc atgttgctga attaaatcgt cttcaggcag atattagagt
    10501 ggaacaaaat atagccagag ttaagggtgt aaaaaaatta agcggtgccg aagttatggc
    10561 aactgatctt cgtgccagcg ctgctttagt gcttgctggt atcgcagctg aaggaaaaac
    10621 tgttatctct cgtatctatc atattgaccg tggttatgac catatagaaa aaaaattaaa
    10681 tgctattggg gctaaaataa cccgttcctg attcaatact aaaaaaacct ataatgatac
    10741 cataagaatc taaaatagat tttcttttct ttttgagagt ctaagtaaaa aaccattaaa
    10801 aagcccattc agaactgtct ttcttaccat ataaaataaa gcatatcaat cttgataagc
    10861 ttgatagaca aaatatcttg ctattttctt cattaatttt tttctttaca ggataagcta
    10921 aatagtgata gtggttagtt gtggttttga acttaccata tagatgataa gtaggagata
    10981 aaatcgcaag ttctggttta ggttggataa gctaatatat tgataaatat tataatatat
    11041 aaataaagag gctatatgga gaaggaatgg aaaatgccac ctgagctctc tgaagaggat
    11101 aaacagaaga ttaaagatat ttctgtttct ttgaaatgtc caaagattgt ggcagaatta
    11161 ctttatagaa aaggattaat aacaattgag gaaatagatg cctttttaca tcctcgttta
    11221 gatgggctct ttgatccttt tcttttccct gatatggaga aagctgttca gcggattata
    11281 cgagctatag ataaaggtga aaaaattacc atctatggtg attacgatgt tgatggcaca
    11341 actgctacca ccttactata tttaggtttg aatcggatag gggctaatat agattactat
    11401 atacctcatc ggatgattga tggttatgga ctgtctcttt caagtttaga ccaattaaag
    11461 gaaaatggta ctgcgttgat tataagtgtt gattgtggag ttaacgcttt agaagagatt
    11521 gaagctatca ataaacgagg tatggaaatt ataattacag accatcacaa tccgaaggaa
    11581 gaacttcccg aagcagtagc tattataaat ccaaaacttc caggttgtac ctatcctttc
    11641 ccttatttag caggagttgg agtagcttat aaacttttaa tagcaattta ccaagccaga
    11701 ggattagaat cccttgaaaa cactttaaaa tatatggatt tagtagcagt aggaaccatt
    11761 gctgatatag ttcccttaat aggagaaaat agaatttttg cccatatagg attacagcat
    11821 ttaatagaaa agaaaaatct cggcttgaat gctttagttc agatagcagg cttaaaaagt
    11881 aaagttttag atagttctga tatagtgttt ggcttagctc ctcgtattaa tgctgccgga
    11941 agaatgggtt ctgcttcttt agcggtagaa ctccttattt cacgggatat agaacaaagt
    12001 aaggaactgg cagaaagtat tgaaagattg aattctctga gacagcaaga agaccagaaa
    12061 accttccagg aagcctgtga gatcatagaa aaaaaatact ctaagctgga aactgttccc
    12121 tgtatcgttg tctcttctga tgattggcac cctggagtta taggaatagt tgcttccaaa
    12181 ctggcagaaa aatattaccg tcctgtttta atgatttctt tcaaagaggg ctggggttgc
    12241 ggttctggaa gaagtgtggc agattttgac ctattcaatg ctttaaaagc cgtagaacac
    12301 aatcttccca gttatggagg tcataaatat gccgtgggat tcactatatt acaggaatac
    12361 ctggaccgtt ttgagaatga actatcccgt tatattgcag accaccttca attagaacaa
    12421 ataaaaccat ctctggaaat agaccatgag atagagcttt atgatatcag cgaatttttg
    12481 ctggattgga ttgaacaatt tgctcctttc ggacctgaaa atagtcgtcc ggtaatgctg
    12541 actcgtaatg ttactattgc tggttatcct tacaccgtag gcagaaatca tctgaaattg
    12601 aaagtggtca aagatggtgt tcagcttgac cttattggct ataatttagg agattatctt
    12661 cctctcctga aaaagaatat ggaaattgat attgtctata ctctggaata caatcgcttt
    12721 ggggataaaa ttaatatcca gggcaaacta aaagatatcc attggtctaa gtaaaggatt
    12781 ttttatgaaa agatgctctg gaattttcat tattctcctt tctcttttat tcctgggaaa
    12841 ttgcgctgat aatagcttca tagcgcgaag agatatagac cattttgaat tcataagagc
    12901 tatccctgta ggaattgaat atgataaagc aatctacagt gctccaacta attcggtctt
    12961 tgctatgcag agggcaagtc aagagattca tatctatagg gatggcaaaa ggataaattc
    13021 tattggaggt ctgggatttg accgaacaaa tttccagagg ttaaacgata ttggaattga
    13081 tactgatgga ggattacttg ctctggataa ggttacgaaa gtcttgcgaa aattctcacc
    13141 tgagggtctt ttcatttctg aaatcaaatt tgatactctt aatcaacctg aacttttctg
    13201 cattggaaag gatggcacta tttttgtcta tgattcaacc agttcagaaa tcatctcaat
    13261 ctcgccttta gatacaaagg aactttaccg ttttggtaga tttgagatag atcaacctat
    13321 gtatattgat tgcaataacg attttctttt tgcctataac tctaaaaaaa atgtcactta
    13381 tgttttctat ctctatgggc aatataagga aactttaacc ggtcaatttg tttatgactt
    13441 gatcaatcat cctataagcg ttaataatat atatccatta attggtcgtc aaacaccccc
    13501 aaaattaatg actacaaatg ctcagatgct tactttactt tatcctcaag aaatctgtct
    13561 ggttaaaata gtttacaaaa gaggtataga tgcgacgccg taatctactt ttaacttttt
    13621 taagtgcatt aatgttagcc ctttcacgat taccatttca tcttggatgg atggtgtttt
    13681 ttgcctgggt gccttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnctttc
    13801 acgattacca tttcatcttg gatggatggt gttttttgcc tgggtgcctt tactttatgt
    13861 atttgagaca ggaaaagcaa aaagtggtga tttacttcgg atgggcttca ttatgtctat
    13921 cgtctatatg agtattgtat tctactgggt aactctggtt accatacctg gtttgatagg
    13981 aatgatagct ttttatggtt tcgtttatta tatcctcttt tatgctataa atcgcatttt
    14041 ccttgtttta cctaaatggc gatatctggg gtttatatca attatgataa cctttgagta
    14101 tattcagaat tttggagaga cccgtttccc ctggttcaat ttagcctatt ctttagctga
    14161 ttatactcat cttattcaat ttgctgatat tggaggtgtt gtcggtctat ctgctttaat
    14221 cctgttaata aatatattga ttcatttatt gcttcccact agtcaaaaat tcttagaaaa
    14281 ccgcggtcgc aatcattatg taaatacttc accaagaaaa aggcagataa cttctgtggt
    14341 agcaatagtt attatcttaa tcagttggtg gggatatgga gaatactgtt taaaaaatct
    14401 gcctttagaa aagcatgagg caggaatatt tgtggtgcaa ccttccattg acgcaagaga
    14461 acgctggaaa cctgaacaat atgataagtc cctggagctt ttccgtcaac taactttgga
    14521 ggcagttgca gattctgcta aactggtaat ctggcctgaa ggagctatga taggtgattt
    14581 agccagtgat tatgctgctc aacgcgacct tcagagaatt atagatgaag ctcaagttga
    14641 tatctttact ggctttcagc attacgaaac agatgtaaat catcctcggg gagaacgaag
    14701 ctataatgct gcctccttat ttcagccaga taattttcat aatgaactat atttcaagaa
    14761 tattcttgtc cccgtagcgg aaagaatgct ctggatggat atatttccct tcttatggaa
    14821 actggattta ggtcaggcaa actgggaatt cggcactaaa ctggcttatt acgaaagcgg
    14881 aggttatcgt ttttcgcctt ctatctgtta tgaactggca ttccctgaaa tctttcatcg
    14941 tatggctatt ccaagagatt ccctttctgg tgagtttaat aaatgcgatt ttctggttaa
    15001 tattactaat gatggatggt tcggaacctc ttatggtcca tggttacatg ctatgatggc
    15061 aagatttaga gccatagaaa atcgcatcca gatttatcgt agtgctaata ctggtatttc
    15121 tctcattgtt gaccctaaag gtagaattct ggcacggacc aaattaaatg aagtatgtgt
    15181 tatcaccgct ccactttata ctactcccaa gataccagta attagaagaa tatataaata
    15241 tcccttcatc tttgttggga tagcccttct tctggcgctt attgccagaa taaaaaaacc
    15301 tggagggtaa cattatgaaa aaatattatt acactattgg ggaagtaagt aacttgctgg
    15361 gtgtaaaacc ttatgtaata cgctattggg aaagcgaatt cagttttctc aaaccgcgca
    15421 aggaatcggg aagaattcgt aaatattccg aggaaaatat tgagctcctg cgtaaaattc
    15481 aagatttact ctataatcaa cactatacta ttgaaggagc gagacaaaaa ttgaaacagg
    15541 aactccgcaa taaaagttcc cacgagccag taccagtttc cccacaaaat aacgcaataa
    15601 atgcagaact ccaggaaaat cttgctgctt tacggcaaaa cctgaaagaa atatgcaatt
    15661 tgtgtcatca attacggagt agaaaaatat gaattttcaa gacctcattt tgaccctgca
    15721 aaattattgg tcagaacaag gatgcaatct gatgcaacct tacgatatgg aaatgggtgc
    15781 aggaactttc catccctcaa ctttttttgg tgctttaggt ggaaaaccgg tttctgtggc
    15841 ttacacacaa ccttgccgtc gtcctaaaga tggaagatat ggagaaaatc ctaatagact
    15901 gcaacattat tttcaattcc aagttattat aaaaccagga ccagataata tccaagatat
    15961 ttatttaaaa agtctggaag ctattggtat agaaatagcc aagcatgata tacgttttgt
    16021 tgaagatgac tgggaatccc caactttagg agcctggggt ttaggctggg aagtctggct
    16081 ggatggaatg gagataagtc aatttaccta ttttcaacaa gtagcaggtt ttgatgtatt
    16141 tcctatcagt gtagaactaa cctatggtct ggaaaggctg gctatgtata ttcaggatgt
    16201 ggatgactac cgaaaattac gctggaacca tcatactctt tatggagaat tgtatttccc
    16261 aagggaagta gaatattcgg aatacaattt caatgccgct gatacttctt tactttttga
    16321 cctctttgct gattatgaaa aggaatgccg tgaattgctt gctaaaaatc ttgtcttccc
    16381 tgcctatgat aatctattgc attgttctca tacctttaat cttctggaag ccaggggagt
    16441 gattagtgta acggaaagag ctgcctatat tgcaagaatt agagcactgg caaaagaagt
    16501 ggctacagct tatcttgcta aatatgacga caaaaccaga taactggaat tataatgata
    16561 tattccgaat ggcactttgg caatgataat attcctcttc tggctacagc aattcataat
    16621 ggacatcact ttcctcttga gcttgtagga ttctgtggag ttgacgaaaa agaccgctta
    16681 cgcgaagaag acccctacac ttcggaattt gcttctcttt cccctaatta tgtagtcaat
    16741 tacacctctc gttttgtggt agacctaaat cggtctttag aaaaagcggt atatttaaag
    16801 cctgaagatt gctggggtct taatccatgg ttaaaaccac ttccagaaga gtatttaaat
    16861 aaactctatg aagattatga tgcctggtat tctttacttc gttatcaaat tgaaagaatg
    16921 ctaaaaactc atccttttct cattatactg gatttacata gctataatta tcttagaaat
    16981 ggaccagaaa ctgaacctga accgaacact ccagatgtaa ttattggcag aagtaatctt
    17041 aaatccgatt actatccttt aattgatagc ttaagagaaa taatggggaa taataccctc
    17101 cagaatagga aattagattg tcagctggat gtaaaattca ccggaggaaa tctttcgcaa
    17161 tggctgaatg cccattatca ggacaaactt atatgtctgg ctatagaatt taaaaagata
    17221 tttatggatg aatggacggg tgaactttat cccactactt tcactcagct caagactcta
    17281 tttaaagaat ccatcgcttc ctggctacag aactctttca atattcagct aacttaatta
    17341 aagaaactat tcttcctttt ttgtcgcctg acttttagct cttaacattt ctggagttgg
    17401 cttggcatta tcatctcttt tccagcgatc gtcaatcttt gtatgtactg gactgtgttg
    17461 ttgaaagtaa tgcacaatag catcgcgaag gtttatctga tgataaatga tattctcttt
    17521 aggaacctga gtgagcaaag ttaagcctgc attgccttcc attaagaagt cagtagtaac
    17581 gcaggtatag attttattag gatcccaggg ttcacctccg actttcaaag aagttactct
    17641 gtcgtaattg ggacgctttt tagaataaac tacattaact cctgaaacaa ttagaccgtg
    17701 ccgagaacct tcaaccctgg tttcaataat acgacgaaga aattctccat cacaggtaaa
    17761 acttaccagc atattgtcaa aaggcataac ttcaaaaata tctctataag ttactggacc
    17821 agcttttata tctgctctaa ctcctcccag attaagaaag gcaaaatcag cctttacttc
    17881 attacgcata gcatcaacaa tcatattgcc taccagactt tgagcatcat aattggttct
    17941 ggttaaattt actgctgcat aacctacaac ctcatccatt ccttcttcag cttttgctac
    18001 ctgttcttca atgataggtg agatttctgc atctggaatg aactcttctt caaatagagt
    18061 tatcatcatt ccatcggtta aagcaggaag tttataacct gagacacttt tggtctcaga
    18121 atcaatcgtg agcgtcatcc atcccagatt tgagccataa gcataacctt ggataaccat
    18181 agtatgagtg taagggtcta tccaaggact gggaattcct ttatgtatat gacctccaac
    18241 aaaaaggtca attccgggaa cttctcttgc caattcctga gcatcatagc cccaaactgc
    18301 atatctttca gtaaagagtg gattaccttt attatcataa cgcgttaaat aggtctcaat
    18361 attatcatag ggtaatccgg catgaccaag aactatcaca atatctgcct gttcttcttc
    18421 ccgaacaatc tttacatatt tggttaaaga tcccttttca tcaagaaaat caaggttttt
    18481 tatattttct gggaaactca tcttttctgt atctgtagtg gtaagaccaa taattgctat
    18541 acggacaccc aatctatcta ctatgatata aggaacaaca taagggggga aatctcctgt
    18601 tcttttatct atgatattac aagaaactac aggaaacttt gccagggcga gagtctcttt
    18661 tagctcgtct tcggttatat caaattcatg attacccaga gtcattgcat catagccaat
    18721 agcattcata tattcaatga tagctttccc cttagttatt gtccctacag gtctcccctg
    18781 gaaaaaatca ccaatatcaa aaagtagtgt agacctctgg gcattgctta aagaacgagc
    18841 atattttatg agagttgcag cagaggctcc tccacctaaa cgaggaggga agtcaggatt
    18901 aataaaggtt gcctcagagc ggtctattcc tccatgcata tcattggtga acataatatc
    18961 cagatatagg tcttccgcaa aaatgcccga ggcaagaaag aggaaggaaa ggatgattaa
    19021 tattattttg cttttcattg tttttaccag gtatagctta gactggtgct aaggctgata
    19081 ttactttccc taactttatc aggattttcc cagcctctat cagttaaagc gatatgttga
    19141 ttaggccaga gctgataact gttttcacca gtataatgac ggtcatcata ataggaatca
    19201 cggaagaggt ctaaagcctc atattctctc caggaatatc caaatccaag gtccagatga
    19261 agtttatcag ttaagggaat actgcttcca ccggttatca taggtgttat tattctttta
    19321 actataatag aattatctgc ttccaccgtt ctgagatagc tattggtagc ttgaaatccg
    19381 aagcggaaag gtaaactata tttaatatta tgttccactc cagcatagaa atttatcaca
    19441 tcatcatatt ggtctcctat ttcagaaaac cgaacatatt caccttcaag attgaaccag
    19501 gtgcgcataa tatttcttgg ttgatagtta atacctaaac gaaactcact gggtaagata
    19561 taatcttcct tagcattagt agtagaactg acttctactc caccataagt tttaacccaa
    19621 tcaatagttc tatccagagt ggtcttaaat gtataggttg cacctattcc aaggcgctta
    19681 tttatgcgag ctgacactcc tgttttatat ctataaccat cccaatctac attttcttta
    19741 taaatagaat ctggtaacac tccttcacct accgtatcaa ttgcccattg agtccagaga
    19801 atatcttttt gatatttagt attcccccag agcttaccaa aatcaaaacc cagatttagt
    19861 tctgaatttt cacccagagc aaaacccaga cttagcaccc cagaagcttg atttaacaca
    19921 cctgcatttt ctatggtatt aaccgctatc ttttctggat aattatcatt atcggtatta
    19981 cggttattac gaatttcttc tttataatcc ccatcaaaac tcaaaagagg ctgataatat
    20041 gctcctatgc ctattcttaa tttccagggt ttaaaactta caaatcctgc tgtagcataa
    20101 tcatcataaa aatttagatt ggaagcatag actgagtcat caatataatt atcaaaggaa
    20161 ttatataaag gaactgctct attatcttca ttgcggttca gaaagaaact tccttctaat
    20221 ccagcaggtc tatttattaa agttaggtta gctggattta aagtaatcgc agaaggtctt
    20281 agttcattaa aaataccagt gcttcccatt gcataactac gagcatccag cattccgtag
    20341 cgattaccaa aataatcatc agttatagac caggcagata aaccagctat tataattatt
    20401 gataatataa cacttaaagc taatttctta atcattcttc ctccacatta ataacggtat
    20461 tcaagactca gccggatagt gttttcttta tgttccaccc gttgaaaaag attttcacct
    20521 tgaacgggaa cattatattc tcggatgctg agttcttgag tctgataagt tttatttcta
    20581 tattttaggg ttaaatacat attttgagaa atacgggaat gaagtgccac ccagaatttt
    20641 gcaccttttt ctcccataaa atctatctcc atatcttccc aatcccaatg agaaatccca
    20701 tgtccgaacc aatatatgaa agagccctgg agcttgagat tagtgttgaa gttgtgagta
    20761 taattaacgc ctatataatc acccaccatt agagtttgag ccgctgccat agtattatta
    20821 cccggttcag caggataggt taaagaggtg taaggtggac cccagactgt gttatagcga
    20881 tattcaaact ccagaaaatc acgattggaa agaaaagtgc ggacgctaac tgtatattca
    20941 tccgtcttag aaactcctcg ttctgcataa tcatcatatc tattaacctg attcttatat
    21001 cttaatctta aaccaagtgc atagataggg cgaaattcca gctcagtttg gaaacgaaca
    21061 gaacgacgac catctgttaa tctttcatag atatccagat aacttctatt tatggtaaag
    21121 aaacggtcaa atttatatcg cgtttcaaaa tatattcctt cttctggttg agcttgggaa
    21181 caattcaaat ataaatctga aagcgttgga ttggtgagag catatatatt cttttccagg
    21241 atagtatctt caaagcgttg gtgttcagag taggcattgc tataaggatt atcaaaatca
    21301 acatcataat gccgatatag actaataaaa tagagattat caaattgagt gtgagcactt
    21361 attagatagg ctttaggatc atcacctaat tttaagtccg aaccatcaac actcagttct
    21421 gcatattcac cttcaattga tatattcccc agagtagtgt gtccatcaaa tccaatcact
    21481 cttctgtaat cacgactata agtatctgtc ctggtgctat aaagattggc aatttcggca
    21541 tccatcatct taaatttagg atagtagtag gaatcacgca ccagtaaggt ttttaaatca
    21601 ttatagtccg gcaccataaa ataagcatca tcatacagtg ctgtgtaggt ggtaaaacca
    21661 atcttggtcc caataaaagg attcacttgc agatgagtgc cccagagctt ttctttcaag
    21721 gcatctttgc gataggcaag atttatataa ttggttgcat aaggtgttcc gctttgtaat
    21781 tcttgattaa acatagcttc agcttcacgc aattcatcgt tatcaaaacg gatagtagga
    21841 accacatagc tgaatatttt atctttcccc tcttctttaa ctgtattgcc atattcatcc
    21901 acatacacaa ttgcatcttt atcatcctga gagaaccaga aagataaacc agcataggga
    21961 tgggtaagct caaaagctgc accacgaaga gcatattctt gtgtgcgaga aaggtccgga
    22021 gtgataccaa gaattctttt actgaagcca tatccagttt tccgggcact atagaaatca
    22081 gtattttcca tcactaatcc ttctccgtaa gtaaccctat agtttcccag atatattttc
    22141 aaacgcgtat tatccagcag aggaagctca ttattttcgt agcctgcata gaacttggaa
    22201 tctgccagaa tttccttggt tgtcttatca cttaaagagg tttctccttt tgccttataa
    22261 tgcatccagc caattttata attattccca tatctggcac gtagcttaac cataacatca
    22321 gggtctatct tatccatatt gaaatagccc cagtaagata ggttcttatg ttgaggaacg
    22381 actatgctgg aattaccata atcattccgt aagaatggct catgatacat atctgaagca
    22441 ccttcttcca gataacgagt gtaatattgc aattccgcat cccagaaaaa cctgttcttt
    22501 actggtggct ctttataata tacatagcta cgtaaattgg tatagccata ataggataga
    22561 cctgtggtat tgcgaagatc tcgcatatca gcaatggtat cacctaaagc cactctctta
    22621 agaatgctta cagcatcaac ggaagagaca ttgggtagac tgatgaaatc gtcaaaatgc
    22681 attttattga cattatgtgg ggtcatcaaa tagtcctgcc atacatcaga cataccttct
    22741 gatgaacctt cattgctatc cagcctttcc aataaatcct ggatttgttg ccttctcgct
    22801 aatgcctcat cttcttcctg ataaattgaa accactacca gatctttgag attgagtagc
    22861 gtcttctggt ctatagaagg aatttctcgt aattgataca catctttaaa tattgaaaca
    22921 tattctctat attccagaat gtctcttgcc tgcttttccg taattggaag ttgcatcaac
    22981 tcctcaaaag ttgcagtatt caaatctatc tttgcaacta acggcatcac gcataacagg
    23041 acaaagaaaa agacaataaa ctttttcatt acctatcctt ataataagtt atatattgaa
    23101 agggaaacct tatttcttat gaaattcaat aaactcaaac tctataatct gtaaaggatt
    23161 ttttatttaa atttatagac ctgcgtttgt ggatagtcca aataatcata ttctccatct
    23221 atatttaaag taacctcacg atcaaaggta tttctttaaa attcataaca aaaccctatc
    23281 cccgataaat caggagaaaa attgaaagtt aaacggggtg tatctttaag aactaattga
    23341 gcagtaaaat aaccaattat ggaaccaata aagacatcac tactccagtg tttatcatca
    23401 tttagtcggg aacaggaagt tagtattgct atggtatagg aaaggggagg aacccattta
    23461 ttttctttat attgttccgc aaagataggt gctaaactcc agaccagtgt ggaatgtgca
    23521 gaaggaaaag aatcattatg caatgaaaaa gaacctgtgc tccagaattc ttttccttct
    23581 tctgtttcag gtcttggtct ttgagttata atttttaaag ataaggttaa accttgagct
    23641 aaaactgcac tttttatact caacaatcca gtttccacag ttttatcaga tttaagacaa
    23701 taaccggata aagtcataac ccctaaagct ggaaacatat atttagcatt acccaaaact
    23761 tcaaataccg taaatatatc atccactgac tctatacgat ttctttgata aaggtctcgt
    23821 aaatgttcat cttccagata gagcattcct cctgttaaaa gtaatccacc tgccttcaga
    23881 tagtcatctt tcgtccagtt aaaaggagct gtggctccct tatagatagt ggaaggatag
    23941 gaatatagat attcactaag ataaggaata gtcacttcag ctgataaact ggttataatc
    24001 aacatcgcta agcttataaa gacaaagata taagattttt tcatcgttaa ttcctgaatc
    24061 tggtaactct tatattacct taatataaat caaaactaat caggcaagat aattttaacc
    24121 cttaatcaaa taaagttata ttaataaaac tatgattaga actggataga taagaaacct
    24181 tccctatttg tctattttct gactcttctc ctttatgtta tgttaaaata tcaataaagc
    24241 tcagaccata tatttacagt tagaataaat tatccttggt caaaaacaga ttaatttttt
    24301 gtcttctcta ttaccaaatt ggcaagtatt aaccttaatt ttgaaaataa atttgataag
    24361 ataaaatagc atactatgat tcagacaaaa agaagaaaaa agataaataa tcttcactat
    24421 tataactcta actatttcct tgacttttaa atgcatttca ttgaactggt tataaatctg
    24481 aaataaggta atataggatg aaattatcgg atatagtaaa gatactggat gcagaagtat
    24541 taacaccaga agtaaatctg gatatagaag ttccctgtgc ttttgcttct gatttgataa
    24601 gtgatatatt gatgtgcacc aaagagccaa ctttacttct cacaggtttg accaatcagc
    24661 aggtaattcg cttagcggat atgattgata ttattgggat tgtatttgta cgcggaaaga
    24721 aaccaccagc agaagttata gagatggcaa aagaacgcaa tcttccctta atttgcacga
    24781 aaaacacgat gtaccgctcc agcggtctat tgtttaatgc tggattacgc agttgccgaa
    24841 tctgaaatgg cggaatattg gtacatccgc cctggatatg aaaaagctgt gcaaaagcat
    24901 tttgaaaatc agcaagaacc tgaagtaagt ctggaatcct tgattagaga aaaagatttt
    24961 aatgaagcag gaaagggctc tgcagaagtt aaaaatgtgt taaaacgcct cggaattgac
    25021 cctgccattc taagacgtat tgctgtggct tcctatgaag ctgagattaa tgtggcagca
    25081 cattcgcttg gtggtgctat gctcagtcat atttatcaag atagaatcca tatgattttt
    25141 atggataatg gaccgggtat tgccgatatt gaacaggcta tgatgccggg cttttccact
    25201 gctgatgaga tggttagaga acttggtttc ggtgctgggt taggtttgcc caatattcag
    25261 aaaaatgttg atgctctcca tattgtctca gaagcagggg atagcactat gctggaattt
    25321 ttaattttct ttaacgataa tggacaataa agaatacttt catgcagttc acatcataga
    25381 ggataattgc acgggttgca catcctgcgt tagagtttgt gccacggaag ctatccgggt
    25441 gcgtaatcgt aaagctcata tagatcccgc gagatgcgta gattgtggca actgtattgt
    25501 tgcctgcaag tttcacgcta ttatcccaca aagtgaccct ctggaattaa taaataaatt
    25561 taaatataaa gtagccatag tatccacttc ctacttcggt caattttcag aagatatatc
    25621 ctatgaggtt gccaaacaag ctatttatga acttggcttt gatcaggtgg aagaagaggc
    25681 aatggtaacc gattttatga ttcagatgat acgggattat gtccgtaaac atagagataa
    25741 aagaccaatc ctttccagta attgtcccac ggtagtaaga ttaatccagt taaaatttcc
    25801 ctcattacta cccaatatct tccaaattga agctccaatg agtatcttaa ctcgctatct
    25861 gagagataag atacaaaaag agaaaggatt aaaagaagat gagattggca tctttctcat
    25921 agttccttgt cctgctcatg ttaccgctgt ccaccaacct gaaggtgcgt ataagcattt
    25981 gcaggatggt gcttttgcca taagtatgat ttatggtaaa gtgcacgaac atattaaaag
    26041 actccagaat caacctaccc ctgatattgt cacctatcct aaaggtctaa cttgggctct
    26101 ttctggtgtt caagctgacc tggttgattg cgaagatatc cgcgctctct cagttaatgg
    26161 catagacaat gtgattgaga tactttctcg ggttgaagac cattatttag accagtatga
    26221 ctatatcgtg cttcgcagtt gcaccaatgg ttgtgtaggt ggatttttta atgtggaaaa
    26281 tccttttgta gcaatgagca gaatcaagaa aatgattaaa gaaggaaaag atacgccgac
    26341 agatttaaat gaattggagc tgctctataa agcaggagaa tttgctgtgg cacctctttc
    26401 tcctcgtcct ataatggaac tggataagga tatcaaaacg gcaatagtca aaatgaaaca
    26461 attaaatgaa attctgacca cactacctgg tttaaattgc tgtgcctgtg gttctcccag
    26521 ttgctatgct ttagcagaag atatagtgca gggtaaagcc acgattgatg attgtctggt
    26581 tctgctcaaa cgtcatacct tagaaaacat tcaagacgat atcttaaaga aaaataaatc
    26641 caaggaatag actaatgcct cgtttatcag aaatacagga tttaatagaa ggaataaatc
    26701 tcactcctga gcttgacccc actcagattg aaatcacagg tggttatgca ggtgatatgc
    26761 ttagtgatgt tatgggttcc gctaaaagcg gtcaagcctg gataactatt atgaa
//
LOCUS       DAORZF010000021        26437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_1564_length_26437_cov_364.597036, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000021 DAORZF010000000
VERSION     DAORZF010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 26437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26437
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1564_length_26437_cov_364.597036"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="PK015_03740"
     CDS             complement(<1..381)
                     /locus_tag="PK015_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOL33616.1"
                     /translation="MKSYRKEIWMFVDKRRGFVNITSEVENCVQESGIQEGLCLVNPM
                     HITASVFINDDEQGLHQDFERWLEKLAPEKPYAQYAHNGFEDNADAHLKRQIMGREVV
                     VAVTNGRLDLGTWEQIFYGEFDGKR"
     gene            complement(381..1082)
                     /locus_tag="PK015_03745"
     CDS             complement(381..1082)
                     /locus_tag="PK015_03745"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOL33617.1"
                     /translation="MKKKHKIKAIIFDLDGTLLDTVQDIADSMNLALAELGLPEHPVE
                     AYRDFVGNGLYKLVERALPEDKRTPEMIKEVADKFWKIYEEKWPMHTKTYPGILYLIQ
                     LCVAHKIKLAILSNKVHYFTKKMIRYFFRGAMINQGKNPFGVYSGEIPGTPDKPDPTR
                     ALELAAKLKCKPENVALVGDTPIDIQTAKNAGMVAIGAAWGYSSREELEKAGADYIFD
                     TPQEMALEIEKLPIL"
     gene            complement(1107..1922)
                     /locus_tag="PK015_03750"
     CDS             complement(1107..1922)
                     /locus_tag="PK015_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOL33618.1"
                     /translation="MNLVEIFHQYLTPERLNTVIRVILTLAIGIPLIIFVRKLVQKLV
                     RNRLSPQSEQLVVRIVYYSLALILVVMVLNEFGFKVSALLGAAGIIGIAVGFAAQTSI
                     SNIISGIFLISEKPFVVGDVIEVGSIIGEIQSIDLLSLKLKTADNKFVRVPNETVIKT
                     EVTNLTRFPARRVDIKITVSYKENLKKVQELLIKIAEEEPKALKEPQAIVRVDSFGDS
                     GIDFIFGVWGKTSELFSLKTEIMMRIKETFEAENIEIPYPHIVLYADEKNRKN"
     gene            2385..2460
                     /locus_tag="PK015_03755"
     tRNA            2385..2460
                     /locus_tag="PK015_03755"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2418..2420,aa:Phe,seq:gaa)
     gene            2493..4028
                     /locus_tag="PK015_03760"
     CDS             2493..4028
                     /locus_tag="PK015_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33619.1"
                     /translation="MMKTYILSNLRIPSREDITLAEKVAKELHIPPSTFKINRIIHKA
                     LDTRKKNYPVFVYTLELEFSSSPPHHPDLSEFKPLKIEIPPPILINNPHPYIIGMGPA
                     GLFCALAMVENGIQPWLFDRGEPLEDRLIKVQDFWQKGILDENSNVQFGEGGAGTFSD
                     GKLTNRRNDALTQRIFSELIKFGAPEEIAYEALPHLGTDGIQRIVKRIREYLLAKGCK
                     FHYRTSLTDLTLKEGKVAQVALGNQWQEPEIIILALGNAARDTWKMLYERGITLEAKP
                     FAIGFRIEQKQSDINRWIYGNEQWAEVLGAASYRLTCKTGFTFCMCPGGKVIVASSEK
                     NTVVTNGMSFSSREGDTGNSAIVTAVNAQDYGNGIWDGMNLQISLENKAFRKGYLAPA
                     QKAKDFLQNQNGSHNIHSTCLPGIYPFNLNNLFPIAISNRLSSTLKYCNHIFPEFYTN
                     SILIAPETRTSSPIKILRKKNELSSLSAANLYPIGEGSGYSGGIISSAADGWRLGERF
                     RIR"
     gene            complement(4165..4452)
                     /locus_tag="PK015_03765"
     CDS             complement(4165..4452)
                     /locus_tag="PK015_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOL33620.1"
                     /translation="MSRDELIKKMAEAAGITQKAAALALNAMLDGITQSLKKEEKVSL
                     VGFGSFNVAHRKARNGINPKTKKPLKIPARKVPVFKPGKQLKEAVKAGKKK"
     gene            5211..5903
                     /gene="queC"
                     /locus_tag="PK015_03770"
     CDS             5211..5903
                     /gene="queC"
                     /locus_tag="PK015_03770"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HOL33621.1"
                     /translation="MEIKYQKAIILLSGGMDSLVTAAIAKEEAEELYFLHCNYGQLTE
                     KREQKSFEAICQYYKPKESKILHWDWFKEIGGSALTDSSLAVPKQEPSRDIPITYVPF
                     RNANLLCAAVSWAEVIGAEAVYIGAVEEDSSGYPDCREVFFQAFQKVIETGTKNEFPI
                     RIITPVLHLSKKQIVQKGMELGAPFALSWSCYLDNEEACGECESCRLRLKAFAEAGFK
                     DPIVYKIKEEIK"
     gene            5900..6889
                     /locus_tag="PK015_03775"
     CDS             5900..6889
                     /locus_tag="PK015_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HOL33622.1"
                     /translation="MSDLTKKNILIVLTGGTISMKEKGSLGVVPSSELADFLREFPQL
                     NNVANIDVLDYMNIPSPYITPEIMLELAKLIDLKIIDYDGVVITHGTDTLEETAFLCD
                     LVLTTRKPVVFTAAMRSGSDIGLDGPRNLVGAVRVACHPESADKGVLVVMNDEIHTAR
                     DVTKFDTGKVDAFRSGDYGPLGSVDPDAIVYHRISLYRENVWTDKLDPNIDLIKAVAG
                     MDGRYIQTSIATGAKAIVIEAFGRGNLPSRIVPDIKAALEKNILVVICSRTYTGRVLT
                     EYGYEGGGKHLAEIGCLLAGDLKGIKVRLKLMALFGKYGDPDVVKRFFLQSRN"
     gene            6898..8019
                     /locus_tag="PK015_03780"
     CDS             6898..8019
                     /locus_tag="PK015_03780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOL33623.1"
                     /translation="MFESNQKIAFLGPAPPYRGGISQFAFRLAQEFQKSGNEVRMFTF
                     EHQYPELLFPGKGQTAEFSQSLEIDIEKVYTPYNPSTWSKAVKSIEAFAPDLTIISYF
                     LPWFAPSYAWICQHLISTRRICLAHNITFHEKWIGSNILSRQFFKHCDRIIVLSEACL
                     QDLKNTMPEDIADRGLLGFHPIYDCYGESPDDLDIESVEELTALFFGLIKPYKGLDVL
                     IKALKQARIRIPELKLIIAGEVYGKEDKYISLIYQLHLEDAVETHFRYIQEHEIKEFF
                     QRSQVCVLPYKSATQSGVIATAYEFNVPVIASKVGGLSEYVKDEETGILVPPDNPEAL
                     AAALIRYFEEGLFYKMSKNIPSFKQQFSWQRLAEIILQA"
     gene            8019..9302
                     /locus_tag="PK015_03785"
     CDS             8019..9302
                     /locus_tag="PK015_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HOL33624.1"
                     /translation="MRLLLITYYFPPCGGATVQRWLKWLPILVEQGFEVTVLTTKDGD
                     YPVYDESLLAEIPPEVTVIRTKTFKFEKIWKIFTGNKRPIPYGNISKNKEQNLINRIL
                     IWIRLNLIIPDIRKFWNPSALKKAIDYLRITPVDYVITTGPPHSTHLIGLKLKKQVKI
                     KWIADWRDPWTSIYYLQLNPPSRISRFWHKKLETKVAQTADWNVVVSEHLASQLPTNK
                     ISVIYSGYDDKKIPAPVIAQTKEKETTFKLKFIGNITEGQRLDILIQIIAKAFPDEDI
                     ELSFIGTNLSEEYRTLLQNLLPGRYVCKNFIPHKEALQEMQDSQVLLLLINYYPGAEG
                     MLTTKLFEYLASYNKILCLGPHNSEAEKLINRYKAGKCFDINESEDAIEYLRNLYSWW
                     HSGKTLKNDIEVTELSAQNQVLKLIDLIHSLNSQS"
     gene            9975..10241
                     /gene="rpsT"
                     /locus_tag="PK015_03790"
     CDS             9975..10241
                     /gene="rpsT"
                     /locus_tag="PK015_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOL33625.1"
                     /translation="MPQHKSPMKRMKTDAKRHARNNYVRRTIKTLEKKIRTDMSDEER
                     QTLLNEIYSQLDKAAKKGVIHKRTASRRKARVAAFVNKTQTKSE"
     gene            10327..10998
                     /gene="mtgA"
                     /locus_tag="PK015_03795"
     CDS             10327..10998
                     /gene="mtgA"
                     /locus_tag="PK015_03795"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HOL33626.1"
                     /translation="MKKKRNFFVQVLRFIVYAHLFFWTIIATLCLFYNFINPPITPLM
                     IQRKLIRGYECQPRKYIPLEEIPKSIVRMTIIVEDANYYHHFGFDWDMIKKAWEKNKK
                     SGKIRYGASTISNQVARTIFLTTNRNVLRKYLEAQVTVIMELLMTKDRMLELYLNYVE
                     WGKGIYGIETASYYYYGTSCRNLSKTQAMKLVSILSNPIDYTPQTYYRSPSARARYNM
                     LQRYF"
     gene            11009..12301
                     /locus_tag="PK015_03800"
     CDS             11009..12301
                     /locus_tag="PK015_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HOL33627.1"
                     /translation="MDEKFLYHIWDAGHLLTPLKTASGKTLQVKYQGQYNTARGPDFH
                     NVIIELNGEILRGDVEIHLHSYDWIAHNHHEDPHYNQVILHIVMDAGQQQYTIKEDGK
                     TIEILELKNQLSDEIRKLLEKRETSSLNDNITYCDLLSAIDNDALISILTSWGLQRFQ
                     NKVRRFDAALMLSDFDQVLYEGIMEAMGYEKNKHNLLILSQTIPLKNIREWQTDGMTA
                     LELASIYCCSSKLLYLCKHHLKPELYHLLLETYERQRFYARSWDIDWQLFRIRPNSHP
                     LYRLLTMANLLNKTQDQGLLNYFLERVDIERSDSKKAFSEFSHIFKESILPGAETLPR
                     PGKSLISNIYINIFLPISYMFFEKHSESDNCRKILKYYKEFPALEENHILRYMSRYMS
                     DAHYDLINHKTILQQGLLELFHRFCNYHLCSECLASKS"
     gene            12377..13168
                     /locus_tag="PK015_03805"
     CDS             12377..13168
                     /locus_tag="PK015_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33628.1"
                     /translation="MKSKLILILWLILVLGMFSCTKKQSNLPDETFKVSEFRTIPSFK
                     AMFLALDKLEETKLSNLPLAQVVPAQKDTIYYAFGWGVLSSDAQLAIASKNQNQLISV
                     TDQFISLAPQLGLHQFANQLRDAVKPEIKNNNWGLLKDVFYAMQESVDTLMLSNNREK
                     ELTFIYLGGWTETTNQIARLIAKDYSTEKSRTLQTDAWEDLAFNLNLIQNMSKDKSPL
                     LAQTIELVHKIQNTLSSGTAAVLTLEQVETIVEHTTALRELYLKS"
     gene            13187..14302
                     /locus_tag="PK015_03810"
     CDS             13187..14302
                     /locus_tag="PK015_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33629.1"
                     /translation="MKHLHLILLFLLISMVAFANPEEIDTYYAEDAPWDDGTAVLHKW
                     NLSAKWDSLSLQKADSLGVYQEIFNEFSLSGQYKETGLTPDIKHQYRLVLYNKEQGDS
                     LTLFAETTTQQQWFSMDKLPLLILLLIICSSVLYYILSARRGKELFIRRISGLDSIDE
                     AIGRATEKGKPILYVPGISDLNDIQTIASLNILNQVAMKSAEYGTELLVPCRFSMVMS
                     AARETVKDAYLKAGRPDAYKEDNIFYVTDDQFGFVAGVDGIMLREMPAANFFLGAFWA
                     ESLILTETGFITGAIQTAGTGQPHQLPFFVVSCDYTLIGEELYAASAYLSRDPQQLGS
                     LKGQDFGKLLTIILILAGIIAEIAGFGWFKNFFEGVF"
     gene            14305..15003
                     /locus_tag="PK015_03815"
     CDS             14305..15003
                     /locus_tag="PK015_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33630.1"
                     /translation="MKSKIPQLLAFLSGLVILVAAFIPHTPFGMFEETLTNWFMIISS
                     FAILLGQSSLIQSNVAKITQKAPNWKYHIATLVSFGVMLIFGLLWGMENTPGILGQGE
                     KLAESLGSKPFDYLFEYAFMPLSSTMFSLLAFYIASAAYRAFIMRTFESNLLLITAVI
                     VMLGRTSFATILTSWIPDSLHFLRLPELTDFIMQYPNTAAQRAILISAALGVVGASLR
                     IILGIERSYLGGEK"
     gene            15000..15863
                     /locus_tag="PK015_03820"
     CDS             15000..15863
                     /locus_tag="PK015_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33631.1"
                     /translation="MSEDINLTKTLRERRIIFLLLVLAVAIPIIFPFSLKTHSTPLTE
                     KAFNLIESTPAGSPVIISFDYDPSTVTELQPMAIAIIEHAWRKNHRIIAMAMWPQGSQ
                     MADMAFSEVQKKFGEEKVYGVDYVNLGYKPGGMVIIQTMGRNLKTVFPKDTAMNDYDS
                     IPLLKNIKTIKDIKYVVSLSAGDPGLRDWIMTANGKFGIPVAGGTTAVSAPGFLPYVN
                     DQNQLSGLLGGLKAAAEYELLLGYEGTASRGMNPQSIAHLLILALIVAGNIRVWRNRR
                     KEKMAKEVNNG"
     gene            15856..16494
                     /locus_tag="PK015_03825"
     CDS             15856..16494
                     /locus_tag="PK015_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33632.1"
                     /translation="MAELMKTIGIWLSAYFTLSIFSFLYKDNPFYKLAEQIFVGLSAA
                     YWLVYTIYNILIPNLFNKLFTDFAHNWILLIPMALGIMMLMRMFPKLEWLSHYPMAIM
                     VGTTAAIAMLLAFQSDFLSQIAATMVNPFKYSSPVDIVGTILLIIGTICGIYFFYFSK
                     KQEGFYKIPSKVGIWFLMVSFGASFGYTVMARISLLIGRLDFLLRDWLHLIR"
     gene            16560..17261
                     /locus_tag="PK015_03830"
     CDS             16560..17261
                     /locus_tag="PK015_03830"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOL33633.1"
                     /translation="MDLKELVSLSPAIKELIDKISNISQEIMSHNWAEANAGNLSLNI
                     SQEMNELKETNSEWYLVTRTRSRYREMVQDPISSLILIQLQANQEKCYPAEAKPTSEW
                     SSHRRLHQYFLEVGRKDRVIIHCHPSNTILLSQLPFYPNKIRINKMLAEALPELPIYL
                     PSGVGTAFYAPPGSEALATNSLESIGDSKALIWQSHGLLCTGKDLDEALDYMEIVEKA
                     AELAIRKLFLTQLRF"
     gene            17381..19159
                     /locus_tag="PK015_03835"
     CDS             17381..19159
                     /locus_tag="PK015_03835"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOL33634.1"
                     /translation="MKHRVIILLLFILTVSFLGAKSPDPKAVVGKIDNITYTYAEYDK
                     ILSNYYAYYQKKWGHPLSTEEKAQLNNQCWEELVGRYIYDKAIKAGKITITQAELLRE
                     AKKNPPEAVKQIPELKTNGKFDRKKYEDALNNIPEFKKAVLEEVKATYQYQKLLNTIR
                     NEVTVSEDSVYKEWFRDNDLVDAQIICFDANKLTDITATEEEGRTYYESHKEQYRKEN
                     CRSYRYVRIPKAPSTADSLAVKEEVMKIYQDLLAGADFAEMAKAKSQDPGSAEKGGDL
                     GWFGRGQMVPVFEETAFKTSPGQIAEPVLSNYGWHIIQTLDRRQGENGEEVLARHILL
                     RIEPSETTLKQLKMDAFTLYQKAQEKGLQKAAQEMGYKVEESGVFQEKDSFIKGIGRD
                     ANLVSFAFQNPVGSIPNIYYSPNGDAYICELSDSISVYYTPFEDEKSRIMNTVTKNKR
                     NNYMNEYAHNFVQNFTPDQYLEQAAQDSLMVIEITNHKKGDPISSLGKIPDLDNALFE
                     TPVGSFTPLINDQSRWFLAKINKHQVPDPAQWEKNKATLIKEAKEKAGQEHLNKWYLE
                     ERRKVTIIDNRRDFYDLTSTDKVIQL"
     gene            complement(19146..20429)
                     /gene="mtaB"
                     /locus_tag="PK015_03840"
     CDS             complement(19146..20429)
                     /gene="mtaB"
                     /locus_tag="PK015_03840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOL33635.1"
                     /translation="MIKIAIATLGCKTNTAESAIISGQFNPEKYTLVPFDSDADIYII
                     NTCTVTQRTDYKSRNLIRKALKQKNKNPAVKVIVTGCYAQRNFDEIKKLGDIDLIVDN
                     QNKLNLAELLDNADYHFQDIMTVDRFNFKPVQKMIEHTRAFQKIQDGCDFRCYYCAVP
                     YARGPARSAKFSEVITQARMFVENGYKEIVLGGINLGLYEDEGKNLADVVEALEELEG
                     LELIRLSSLEPHLFSLELIKRLRQCTKLCPHFHIPLQSGSDRILKNMGRPYNTRDFEA
                     LLNNILSFFPDAAIGLDVITGFPGEDNSDFQTTLNFISKLSIAYLHIFPFSRRPGTVA
                     YSLPEQVPKPEKNLRAQKLLQLGKQKKEKYKQKLINEKIILRGILEDIENGRGTFLSD
                     HYIRAYTFTNEKAGELLKIIPQQTYRDGVFGTKVE"
     gene            complement(20473..21090)
                     /gene="purN"
                     /locus_tag="PK015_03845"
     CDS             complement(20473..21090)
                     /gene="purN"
                     /locus_tag="PK015_03845"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOL33636.1"
                     /translation="MERTPSKTWRIAVLTSGAGRGSNLQALYEYFKKHSLPVEIAFAT
                     ASRKDAPVVQLCEKLNIPCYILSPKKDKDFENQLLELCQQEKIELIALAGFMNLLSSS
                     FLEKVKIPVLNIHPALLPKYGGKGMYGMNVHNAVFASGDKVSGVTVHLVDPQYDNGPI
                     VAQVKVDISDCKSPEEIAAKVLEIEHQTYAPVIYQFLCKYYAPEK"
     gene            complement(21057..22841)
                     /gene="aspS"
                     /locus_tag="PK015_03850"
     CDS             complement(21057..22841)
                     /gene="aspS"
                     /locus_tag="PK015_03850"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOL33637.1"
                     /translation="MMIDNLGNLKRTDYCGDLRSKDIGKDVTLMGWVNSRRDLGGLIF
                     IDLRDIKGMIQVVIHPEEKESFSKAERLRLEYVVAAKGKVVARTPENVNMNIPTGNIE
                     IKAEEIYLLNDCEPLPIQLNEAALAEEDLRLKYRYLDLRRPSLQKNILMRHQIMQTMR
                     NFLSQEGFYEIETPILMKSTPEGARDYLVPSRIYPGKFYALPQSPQMFKQLLMIAGFD
                     RYFQLARCFRDEDLRADRQPEFTQLDIEMSFVTQEQIFDLLERLMHTLFSEVLNIELP
                     IPFTRLTYREAMDRFGCDKPDLRFALELSDLTSALQNTQFQVFRSAIQQGAVIKALCI
                     PDGADYSRSKQDELISVAKHQGGKGIAFAKVTEKGLESGISKFLTEEESKAIIELTQA
                     NPGDLIAIVADNYEMTSKVLAALRNTIAEWQNLIPDNTFCFVWITDFPLFQYNAEEKR
                     WEPAHHMFTLPREEHIPWLYEPDKYGQIIGQLYDLVCNGVELSSGSIRCHRYDLQRRI
                     FDILGLSEQELNERFGFFLEALKYGTPPHGGIAPGLDRLVMIMTRAESIRDVIAFPKT
                     LKATDLMCGAPSEVSEEQWKELHLKPGE"
     gene            complement(22859..24094)
                     /locus_tag="PK015_03855"
     CDS             complement(22859..24094)
                     /locus_tag="PK015_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOL33638.1"
                     /translation="MKLRKEAIGWMFYDFANSAFTTVIVTVVFSVYFVQTIASGRPEG
                     GEWLWSQAIAISMTLAAIFAPILGAMADYSRSKKRFLLFFTILTIISTALLYFVGQGD
                     LILAMILFIIANFSFNSANVFYDAFLPEVGMKKDLGRISGLGWGFGYIGGLVTLILAL
                     ALIDKHIKLVWPMVALHILIFSSITFLTLKEKKYPVQKKNYFKVALDRVLFSFKHIKK
                     MPDLLNFLISYFLYNIGIYTVIVYAAIFGGAQFGMTQQSLIIFFIIAQVTSFVGAALF
                     GLIADKLNVRLALSISLIIWVVVVVWAFFCNTAQEYYYLGLLAGLAIGSSQANSRTML
                     SLLTPLDKQAEFFGFYTLVGRISAIIGPFVYGEITRLAGNQRYAIISLIVFFIAGWIM
                     LHQVKLERGIKFGQEYVFK"
     gene            complement(24091..25167)
                     /gene="recF"
                     /locus_tag="PK015_03860"
     CDS             complement(24091..25167)
                     /gene="recF"
                     /locus_tag="PK015_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198307.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HOL33639.1"
                     /translation="MILKQLFLVHFRNYENARFDLHPAGNLIIAPNGYGKTNLLEAIA
                     YCGIGKSVHFHRDEFLCEQGYDFFSIKGLFQTDNELGLEIQISWQKGKKLLKINGNPV
                     RQLSRIYEGVKVIYSAPEDMNLIDGSPRFRRQYFDLALSQIYPEYISLLRNYLHIVEQ
                     RNNLLKKEYTYAEKHSWDLHFCEFLFPVLQYRQKYLNLVNQALKEQYPHISEQVKDIS
                     IQYKVNGIEDYPNSIDKLLRILEELEKREKNYQRTLVGPHLDDYEFLLNQNNLKSYGS
                     QGQKRITVIILKLIQASLIENLTSIKPIMLFDDVFAELDQLHTHRIKEFLDFHYQIFI
                     ASPNEQITSEWNELTRLSLPENKQ"
     gene            complement(25172..26344)
                     /gene="dnaN"
                     /locus_tag="PK015_03865"
     CDS             complement(25172..26344)
                     /gene="dnaN"
                     /locus_tag="PK015_03865"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOL33640.1"
                     /translation="MKFAVEKSELVQHIQHLAGIISTKAASPILTNYLIEADEKKGEV
                     KITATDLKITAVVVLNAAVSDSGIIAVSASYFNSIISQMPDSVINIWQNEDLLMIQCN
                     KIEFNILCADYTLFPQLLMPSFDNATLVNAETFNRMISKTVFAVSTDVNRPVLTGVCW
                     KIMEKVNLMAATDGRKVAEIIVPNTAASVTESGEENIDESIFDDPYSGQLLEKIIPVK
                     TLNFLQKIHDPAVKEMKVIFERNNIIFGYADYLVVSNIIDHNYPEYEKAFLAELPNHL
                     ILDKETLMQSIRRIALLAPEDSGRVKFDVDKDHFEISTVNKETGEAKEFIDKYEYNGV
                     PTSISFNNKYLLSILDAIDTEQVLIKLGDSRDPIMVFNEPPLKNQKITFLLMPLRT"
BASE COUNT         8387 a         4876 c         4807 g         8367 t
ORIGIN      
        1 tcgctttccg tcaaattcac catagaagat ttgttcccag gtgccaaggt ctaatctacc
       61 attagttact gccaccacta cttctcttcc cataatttga cgctttaaat gcgcatcggc
      121 attatcttca aagccattat gggcatattg agcataaggt ttttccggag ccagtttttc
      181 cagccatctt tcaaaatcct gatgtaaacc ctgttcatca tcgttgataa acacggaggc
      241 agttatatgc ataggattaa ccaggcataa tccttcttgg atgccacttt cttgaacgca
      301 gttttctact tcagaagtga tattgacgaa tcctcgtctt ttatccacaa acatccagat
      361 ttcttttcgg taacttttca ttagagaatg gggagctttt caatttccag agccatttct
      421 tgtggggtgt caaatatgta gtcagctcct gctttttcca gttcttcacg actgctataa
      481 ccccaggcag cgccaattgc aaccatacca gcattcttag cagtttgaat atcaatgggt
      541 gtatcaccca caagagcaac attttcaggt ttgcatttta gctttgcagc aagttccaaa
      601 gccctggttg gatctggttt atcaggggtt cccggtattt ctccactgta gactccaaaa
      661 ggattcttgc cttgattaat cattgctcca cggaagaaat aacgaatcat cttcttagtg
      721 aaataatgca ctttattgga aagaattgcc agcttaatct tatgcgcaac gcagagttga
      781 ataagatata ggattccagg ataggtcttg gtgtgcatag gccatttttc ttcatagatt
      841 ttccagaact tatccgctac ttcttttatc atttctggag tgcgtttgtc ctctggcaaa
      901 gctctctcca ccaatttata cagcccattc cccacaaaat ccctgtatgc ttccacagga
      961 tgttcaggca aaccgagttc agctaatgcc agattcatac tatcagcaat atcctgaacg
     1021 gtgtccagca atgtgccatc taaatcaaat atgatggctt tgattttgtg tttctttttc
     1081 attttcttta cacttccttt atggttttag ttttttctat ttttttcatc cgcatataaa
     1141 acaatatgcg ggtaaggtat ttcaatattt tctgcttcaa aagtttcttt tattctcatc
     1201 ataatttccg tctttaaaga aaacagctca ctagtcttcc cccaaacgcc aaatataaag
     1261 tctattccag aatctccaaa actatccact ctaactattg cttgaggttc ttttaaagct
     1321 ttaggttctt cttctgctat ttttataagc aattcttgaa ccttttttaa attttcttta
     1381 tacgaaactg taattttgat gtccactctt cgtgccggaa agcgggtcag attggtcact
     1441 tctgttttta taacggtttc attgggcact ctaacaaatt tattatctgc agtttttaac
     1501 tttaaagaaa gaagatctat actctgaatc tcaccaataa tagaaccaac ttcaatcaca
     1561 tctcctacaa caaaaggctt ttccgaaatc aaaaatatac cactgataat gttggaaata
     1621 ctcgtctggg cagcaaaacc aaccgcaatt cctataattc ctgcagctcc aagtaacgca
     1681 gaaaccttaa aaccaaattc atttaaaacc ataactacca aaatcaatgc cagactataa
     1741 tatactattc taactaccaa ctgctcactt tggggtgata atcggttccg aactaacttt
     1801 tgtactaatt tccgtacaaa tattatcagt ggtattccta tggcaagagt cagtattact
     1861 cttattacgg tatttaatct ctctggtgtt aggtattggt ggaagatttc tactaaattc
     1921 attggctaaa tcttattctc gtttgtattt ataaaaataa aaaacaatat gatacactat
     1981 tttgaaatgc tattatttgt ctatcttttt tttatatctt attccaaatt ttcctaaata
     2041 ccactttatt ttattataaa tttatctaaa aatctttctt ttcatttact aactcctttt
     2101 attttaactg ataacagtat gaacatagca taaagaaaaa tttaaattac tttactaatc
     2161 tttttacaca actgctctaa acctaattat aacttaaatt agataaaaat agattgaagt
     2221 ataaacggtt aaagctggtt aaggttatgc taaattgctg aattaatatc ccttatagta
     2281 aagtttatat ccagctttca aaatgagtag ataggacata ataatgcggc tgaaacaatt
     2341 gtcttgacaa atagaacggt ataaaagttt atgcctgtaa gtgtggcgag atagctcaga
     2401 cggtagagca gaggcctgaa aagccttgtg tccccagttc gattctgggt ctcgccacca
     2461 tttatatttt tatcaccaga aagtttgtat ctatgatgaa aacatacatc ttgtccaatt
     2521 tgagaattcc ttcaagggaa gatattacct tggctgaaaa agttgctaaa gaacttcata
     2581 tccctccttc taccttcaaa ataaacagga taatacataa agcattggac accagaaaga
     2641 agaattatcc ggtgtttgtt tatactctgg aactggaatt ttcttcctct ccaccccatc
     2701 atccggattt atcagaattt aaaccgttaa aaatagaaat ccctccaccg attttaatta
     2761 ataatccaca tccttatatt ataggaatgg gtccagctgg actgttttgt gctttagcga
     2821 tggtagaaaa tgggatacag ccatggcttt ttgatagagg agaacctctt gaggatagat
     2881 taataaaggt tcaggatttc tggcagaaag gtattttaga tgaaaattcc aatgtccaat
     2941 ttggtgaagg tggagcagga actttttcgg atggaaaact gaccaacaga agaaatgatg
     3001 ccttaacaca aagaattttt tccgaattaa taaaatttgg agcccctgaa gaaattgcct
     3061 atgaggctct tccccactta ggcacagatg gaattcagag gatagtgaaa agaatccggg
     3121 aatatctttt agctaaaggc tgtaaattcc attaccgaac ttcgttgaca gatttaacat
     3181 tgaaagaagg aaaggttgca caagtggctt tgggaaatca atggcaagaa cctgaaataa
     3241 tcattttagc tctgggtaat gccgcaagag atacctggaa aatgctatac gaaagaggaa
     3301 taactctgga agctaaacct tttgctatag gattccgtat agaacaaaaa caatctgata
     3361 taaatcgctg gatttacgga aatgaacaat gggctgaggt tttaggtgca gcaagctatc
     3421 gccttacttg caaaaccggt tttacctttt gtatgtgtcc aggtggcaaa gttatcgttg
     3481 cttccagtga aaaaaatact gtagtaacca acggtatgag cttttcctca agagaaggtg
     3541 atactggcaa tagtgccatc gtaactgcag tcaatgcgca ggattatgga aatggaatct
     3601 gggacggtat gaatcttcaa ataagcttag agaataaggc atttcgcaag ggatatctcg
     3661 ctccagcaca aaaagctaaa gacttccttc aaaatcagaa tggttctcat aacatccatt
     3721 ccacttgcct tcctggtatc tatccattta atctaaacaa tcttttccct attgctatat
     3781 ctaacagatt atcctcaact ttaaaatatt gtaaccacat attccctgaa ttttacacca
     3841 actcaatttt gattgctccc gaaaccagaa cttcttctcc tattaaaatt ttgagaaaga
     3901 agaatgaact atccagtctt tcagctgcta atctataccc aataggtgaa ggaagtggct
     3961 attcgggtgg aattattagt agtgcagcag atggatggag attaggagaa agatttagaa
     4021 ttcgttaatc atcatatata aaagctataa tactcttttt atcttactat tttatttgtc
     4081 ccaattaaaa tatccttgtt ttctccgaat ataaaaaagg tgggagatta gtcccacctt
     4141 ttgtcatgat gtcaattcat taggttattt cttttttcca gccttaacag cttctttcag
     4201 ctgctttcca ggtttgaata ctggcacttt acgagctggg atcttcaatg gctttttagt
     4261 cttgggattg attccattgc gagccttacg gtgcgcaaca ttaaaggaac caaaacctac
     4321 taacgacact ttctcttctt ttttaagaga ttgtgtgatg ccatctaaca tagcgttgag
     4381 tgcaagtgca gcagcttttt gggtaatacc tgcagcttct gccatctttt taattaattc
     4441 atctctgctc attttacctc cttatggatt gtatttaaag agttagagat tatacaaagt
     4501 ctattgaatt caggttctat ataatttgtc aataaaaaaa tgatgatttt acagcatatt
     4561 aagcatttat gtcgtatcaa aaattccacc tctaattata actcattgta ttacatagag
     4621 atataaacat agccttaaat ataaatttcc cctgtttttt tctctctttt ccattatata
     4681 aacggattta tttataatta tctagtgtaa ataatggaac aaaaacccaa aatattcccc
     4741 aataaagtat taaaaaccat agtgaagatt tacaaaaaag cactctattt ttcttgaaat
     4801 ttagacctct tatttttata ataatactta aaatattaaa aaatgaatgg catagaaaag
     4861 gggttacttt acaactttac taagcatttt ggatagatgg tgtctttttt ctgaaattga
     4921 aatcattaaa gcaagtaata tattgatagc taagtctaag aatgatagtc atctaatgag
     4981 gttgttaaac caacagtgtt ttgattcaga aggtaagata aataaaagaa gacaaaatta
     5041 cttattagaa tacattcatc aattaaataa tacctaaaat catttaattt ctaatcataa
     5101 attaaatctt cctctcttta cacaaactgc tttcttgagt gtaaatgagt tatcaattca
     5161 gcacttgaca cttttacccc ttagtttagt caggattaaa agagcaaatt atggaaataa
     5221 aatatcaaaa agctatcatt ttactaagtg gtggaatgga tagtctggtt acagctgcca
     5281 ttgctaaaga ggaagctgag gagctatatt ttctccattg caattatggt caattgacag
     5341 aaaagcgtga gcagaaaagc tttgaagcca tatgtcagta ttacaaaccg aaagaaagca
     5401 aaatactgca ctgggattgg tttaaagaaa ttggaggttc agctttaacc gattcttctt
     5461 tagccgttcc taaacaggaa ccttccagag atattcctat cacctatgtt cctttccgca
     5521 acgccaattt actttgtgct gcagtatcct gggcagaggt cattggtgca gaagcggtct
     5581 atattggagc agtagaagaa gatagtagtg gctatccaga ttgtagggag gtatttttcc
     5641 aggcttttca aaaagtgatt gaaacaggca ccaaaaatga attccctatt cggattatca
     5701 ctcctgttct acatttatcc aaaaaacaaa tagtgcaaaa agggatggaa ttaggtgcac
     5761 catttgcact ttcctggagc tgttatttag ataatgagga agcctgtggc gaatgtgaaa
     5821 gctgtagatt gcgtttaaaa gcttttgctg aagcaggttt taaagatcct atcgtatata
     5881 aaataaaaga agagataaaa tgagtgattt aacgaaaaag aacatcctga ttgttttgac
     5941 cggtggaact atttctatga aggagaaagg gtctttaggt gtagtgccca gctcagaact
     6001 ggcagatttc ttacgggaat ttcctcaatt aaataatgtg gcaaacattg atgtgctgga
     6061 ctatatgaat attcccagtc cttatattac tccagagata atgctggaat tggcaaagct
     6121 gatagatttg aaaatcattg attatgatgg agtagttata actcacggca cggatactct
     6181 tgaggaaaca gcattcctct gcgatttggt tctgaccacc agaaaacctg tagtattcac
     6241 tgcagcaatg agaagtggaa gtgatattgg tctggacgga cccagaaatc ttgtgggtgc
     6301 tgttagagtt gcctgtcatc ctgaatctgc tgataagggt gtgttagtgg ttatgaatga
     6361 tgagattcat actgctcgtg atgtcactaa atttgacact ggtaaagtgg atgcatttcg
     6421 ttcaggagat tatggtcctc tgggaagtgt tgatcctgat gcaattgtct atcataggat
     6481 ttcgctttat agagagaatg tctggacaga taaacttgat ccgaacattg atttaattaa
     6541 agcggtagct ggaatggatg gaagatacat tcagacttcc attgccacag gtgcaaaggc
     6601 tattgtgatt gaagcttttg gacgaggaaa tcttccttcc agaatcgttc ctgatattaa
     6661 agcagccctg gagaaaaaca ttttggtggt tatctgttct cgtacttata ctggaagagt
     6721 gcttacagaa tatggatatg aaggtggtgg caaacattta gcagagatag gctgtctttt
     6781 agcaggagat ttgaagggga taaaagttcg tctgaaactt atggcactat ttggtaaata
     6841 tggtgaccct gatgtagtta aacgtttctt cctgcagagt agaaactgat ttgtaatatg
     6901 tttgaatcca atcaaaagat tgctttttta ggtccagcac ctccttatcg tggagggatt
     6961 tcacaatttg ctttccgtct tgctcaagaa tttcagaaaa gtggaaatga agttcggatg
     7021 ttcacttttg aacatcaata tcccgagttg ttattccctg gtaagggaca gacggcagaa
     7081 tttagtcaat ctctggaaat tgacatagaa aaagtttata ctccttataa tccttccacc
     7141 tggtccaaag cagtaaaaag tatagaagct tttgcacctg atttaaccat tatttcatat
     7201 tttctgccct ggtttgctcc ttcttatgct tggatttgtc agcatctaat atctaccaga
     7261 agaatatgtc tggctcataa tataaccttt catgaaaaat ggattggctc aaatatcctt
     7321 tcccgtcaat tctttaagca ttgcgatagg attatagttc tttctgaagc ctgtcttcaa
     7381 gacctgaaga atacaatgcc agaagatatt gcagatagag gtcttctggg atttcatcct
     7441 atctatgatt gttatggaga aagccctgac gatttggata tagagagcgt ggaagaactt
     7501 actgctctgt tttttggact tatcaaacct tataaaggtc tggatgtttt aattaaggca
     7561 ttaaaacaag ccagaataag gattcccgag ctaaaactta ttatcgccgg tgaagtatac
     7621 ggaaaagaag ataaatatat ctctttgatt tatcaattgc atctggaaga tgcggtagag
     7681 acacatttcc gatatattca agaacacgag attaaagagt ttttccagcg tagtcaagtc
     7741 tgtgtgcttc cttataaaag tgctactcaa agtggagtta ttgccactgc ttatgaattt
     7801 aatgtgcccg ttattgcttc aaaggttgga gggctaagtg aatatgtgaa agatgaagag
     7861 accggcattt tagttccacc tgataatcca gaagctttag cagcagcctt aattcgctat
     7921 tttgaagaag gattgtttta taagatgagt aaaaatatac catctttcaa gcagcagttt
     7981 tcctggcaaa gattagcaga aataatttta caagcctgat gcgtttactt ttaatcacct
     8041 attattttcc tccttgtgga ggagctactg ttcaaagatg gcttaaatgg cttcctattc
     8101 tggttgaaca aggatttgaa gtaaccgttc ttactactaa agatggcgat tatcctgtct
     8161 atgatgaaag tcttttagcc gaaatccctc cagaagtaac ggttattcgg actaaaacct
     8221 ttaaatttga aaaaatatgg aaaatcttta ctggtaataa aagacccata ccttatggaa
     8281 acataagcaa aaacaaagaa caaaacctca ttaatagaat ccttatttgg ataagactca
     8341 atcttattat tcccgatatc agaaaattct ggaatccctc tgctctgaaa aaagcaattg
     8401 attatcttcg tattactcct gttgattatg tgattactac tggacctcct cattctactc
     8461 atcttattgg actaaagcta aaaaagcaag taaaaataaa gtggattgcg gattggcgcg
     8521 atccttggac ctctatttat tacttacaac taaatccacc atctcgtatt agccgtttct
     8581 ggcataaaaa actggaaaca aaagttgctc aaacggctga ttggaatgtt gtggtgtctg
     8641 aacatctggc ttctcaatta cccactaata aaatttcagt aatctatagt ggttatgatg
     8701 ataaaaagat acctgcccca gttattgctc agacaaaaga gaaagaaact acctttaagt
     8761 tgaagtttat aggaaacatt actgagggtc aaaggctgga tattctgatt caaataatcg
     8821 ctaaagcttt tcccgatgag gatattgaac taagttttat tgggactaat ctttctgaag
     8881 aataccgcac tttactacag aatctattgc ctggaagata tgtttgtaaa aattttatac
     8941 ctcataaaga agcacttcag gaaatgcagg attctcaagt tttgctctta ctgattaatt
     9001 attatccagg tgcagaagga atgctcacta caaaactttt tgaatatctt gcctcttata
     9061 ataagattct atgcttagga cctcataata gtgaagctga aaagcttata aatcgctata
     9121 aggcagggaa atgctttgat ataaacgaat ctgaagacgc aattgaatat ctgagaaatt
     9181 tatacagctg gtggcattca ggaaaaacct tgaaaaatga tatagaggtt acagaactta
     9241 gcgctcaaaa tcaagtctta aaacttatag atttaataca tagcttaaat agtcagtctt
     9301 aattaaattt tcattattta ttccgttaaa gccctatttt ggataatata tatagtccaa
     9361 agaaaataca attttttgct attaatgaat tcattatgac tttaataatc accagattag
     9421 tttgttaaaa ggaattatat atatatccag aaattggttt ccttttaaat tgatacctat
     9481 aagtttttat tttatggtgt tttccttgta tgcaatattt agagtataaa tcctatgatg
     9541 tcggatttta agcaaaagtt ttagccttat atttatatat cattccccct taacagtaac
     9601 tgcatagata atatctaact gataaggaac ttatgtccac tttcggacat aagctggaca
     9661 taagttggac ataagttgga cataagttgg acataagcta aagattaaaa ggttagattt
     9721 cagattcttt tttaagatag gacaacaatt taagactatg ctaaataata ccataagtaa
     9781 tttataatat aagttttatg ctatatactg tccgctttaa taaaaaagat ttattaagtc
     9841 attttaggaa ttctattatt ttataaaaag tagtttaggt tataaataag ataagaagtt
     9901 cccacaaagg cattgacaaa aaagcgagcc atttatatat ggaatcaaaa tattaagaga
     9961 atatgaggta gaaaatgccc caacacaaat caccaatgaa aagaatgaaa actgatgcca
    10021 aaaggcatgc acggaacaat tatgtccgtc gtactatcaa aactttagaa aagaaaattc
    10081 gtactgatat gagtgatgaa gaaagacaaa ctttattgaa tgagatttat tcccagctgg
    10141 acaaggcagc aaagaagggt gttatccata aacgtacggc ttctcgtcgc aaagctcgtg
    10201 tagctgcttt tgttaataaa acccaaacta aatctgaata gcttttaatg gtcaggttat
    10261 tatatatcta tttgtagctg agtttgttct gaattaatta tttagtttca gggttgtgtg
    10321 tattgaatga aaaagaaacg caatttcttt gttcaggttc tgcgtttcat agtttatgct
    10381 catctctttt tttggactat tattgccacc ctctgcctct tttacaattt tatcaatcct
    10441 cctataacgc ctttaatgat acaacgaaaa ctgattagag gttacgaatg tcaaccccga
    10501 aaatatatcc ccttagaaga aatccccaaa tccattgtaa ggatgaccat tatcgtggaa
    10561 gatgccaatt attaccatca ttttggattt gattgggata tgattaagaa agcgtgggag
    10621 aaaaataaga aaagtggaaa gatacgttac ggtgcaagta ctatcagtaa tcaggtagcc
    10681 agaaccatat ttcttactac gaataggaat gtcctgcgaa aatatctgga agctcaggtc
    10741 accgtgatta tggaattgct tatgactaag gataggatgc tggaattgta tcttaattat
    10801 gtagaatggg gcaagggaat ttatggcata gaaactgcca gttactatta ttatgggacc
    10861 agttgtcgta atctaagtaa aactcaagct atgaaactgg tgagcattct ttctaatcca
    10921 atagattaca cacctcaaac ctattatcgt tctccttctg ctcgtgcacg atataatatg
    10981 ttgcagcgat atttttgaga gggactaaat ggatgagaaa tttctctatc atatctggga
    11041 tgcaggtcat ctactcactc ctctaaaaac tgccagtggc aagactcttc aggttaaata
    11101 tcaaggtcaa tacaatactg cacgaggtcc tgatttccat aatgtaatca ttgaactaaa
    11161 tggagaaatt ctccgtggag atgttgaaat ccatcttcac agctatgatt ggatagctca
    11221 taaccatcat gaagaccccc actacaatca ggttatcctg catattgtga tggatgcagg
    11281 acaacagcaa tatacgataa aagaagatgg aaaaaccata gaaattctgg aactgaaaaa
    11341 tcagcttagt gatgaaatac gaaaactgct ggaaaagcgg gaaacatcct ctttaaatga
    11401 taatattacc tactgtgatt tgctttctgc tattgataac gatgctttaa ttagtattct
    11461 taccagctgg ggtcttcagc gcttccagaa taaggtaaga agatttgatg ctgctttaat
    11521 gttgagtgat tttgaccagg ttctctatga aggaataatg gaagctatgg gttatgagaa
    11581 aaataagcat aacttactca ttctatctca gactatccct ctaaaaaata tccgagaatg
    11641 gcaaactgat gggatgactg ctctggaact tgcttccatt tattgttgtt ccagtaaact
    11701 tctttatctc tgtaagcacc acctgaaacc tgagctctat catttgcttc tggaaaccta
    11761 tgaacggcaa agattctatg ctcgctcttg ggatatagac tggcaacttt tccgcatccg
    11821 tcctaatagc catcctcttt atagactgct cactatggca aatctactga ataaaactca
    11881 agatcagggc ttattgaatt attttctgga acgagtggat atagaaagaa gtgatagcaa
    11941 gaaagctttc tctgagtttt cccacatttt taaggaatcc atccttcctg gagctgaaac
    12001 tctgcccaga cctggaaaaa gcttaatcag caatatctac atcaatattt ttctacctat
    12061 cagttatatg ttctttgaaa agcattctga atctgataac tgccggaaga tactaaaata
    12121 ttataaagaa tttcctgccc tggaagaaaa tcacatccta cgctatatga gcagatatat
    12181 gagtgatgca cattatgatt taataaatca caaaactatt cttcagcagg gattactgga
    12241 actatttcat cgcttttgca actatcatct ctgttcagaa tgtcttgcca gtaaaagcta
    12301 aatcagaaaa gaggaatata tagaatgtag tttagtaact ttatatataa aaatgatatt
    12361 gaaaaggaag ataattatga aaagtaaact tatccttatc ctatggttaa tcctggtatt
    12421 aggaatgttt tcctgcacaa aaaaacaaag caacctacca gatgaaactt ttaaggtgag
    12481 tgaattcagg actattccct ctttcaaagc aatgtttctt gctttagata agctggaaga
    12541 aactaaactt tccaacttac ctttagctca ggtcgtccct gctcaaaagg acactatata
    12601 ttatgctttt ggctggggag tactttcttc tgatgctcaa cttgccatag ccagtaaaaa
    12661 tcaaaatcaa ctaatctcgg taactgatca gtttatctct cttgcacctc agcttggtct
    12721 ccaccagttt gctaatcaat taagagatgc agtaaagcct gagataaaga ataacaattg
    12781 gggattgtta aaagatgttt tctatgctat gcaagaaagt gtggatacct tgatgttatc
    12841 aaacaataga gaaaaagagc tcacttttat atatctcggt ggctggacag aaactaccaa
    12901 tcagatagcc agactaatcg caaaggatta tagtacggaa aaatccagga ccttacaaac
    12961 tgatgcctgg gaagatcttg cttttaatct caaccttatc cagaatatgt ctaaagataa
    13021 atcacctttg cttgcccaaa ccatagaatt agtacataaa attcagaata ctctttcttc
    13081 tggaacagcg gcagttttaa ctctggaaca ggttgagacc attgttgaac atactaccgc
    13141 tctgagggag ttatacttga aatcttaagc ttttattgag gtgaatatga aacatttaca
    13201 ccttatcctg ttatttttac tcatcagtat ggttgctttt gccaatcctg aggaaattga
    13261 cacttattat gctgaagatg caccttggga tgatggaaca gccgtgcttc ataagtggaa
    13321 tttaagtgct aaatgggatt ctttaagttt gcaaaaagct gattcgcttg gtgtctatca
    13381 agagatattt aatgaatttt cactgtccgg tcaatacaaa gaaaccggtc tcactccaga
    13441 catcaaacat cagtaccgct tggtgttata taataaagaa caaggggata gtctcactct
    13501 ttttgctgaa actaccactc aacagcaatg gttttctatg gataaacttc cactcctgat
    13561 tcttctgcta atcatttgca gttccgttct ttattatatt ctatctgctc gtagaggaaa
    13621 agaactattt atccgtagga ttagtggtct ggatagcata gatgaagcca ttggaagagc
    13681 cacagaaaag ggaaaaccta tactctatgt tcctggcatc agtgacctta atgatattca
    13741 aaccatagca tctttgaata tcttgaacca agtggcaatg aaatctgctg aatacggcac
    13801 tgagctcctt gtgccttgtc gcttttctat ggttatgtcc gctgctcgtg agacggttaa
    13861 agatgcctat cttaaagcag gcagacctga tgcttataaa gaggataata tcttttatgt
    13921 aacagatgac cagtttggat ttgtggctgg agtagatgga ataatgcttc gtgaaatgcc
    13981 agcagcaaat ttctttcttg gagctttctg ggctgaatct ctaatcctaa cagaaactgg
    14041 atttatcact ggagctattc aaactgctgg aactggacaa cctcatcaat tacctttctt
    14101 tgtcgtttct tgtgattata ccttgattgg agaagaatta tatgctgctt ctgcctattt
    14161 atcaagagac ccgcaacaat taggtagcct aaaaggacaa gattttggta aattactcac
    14221 catcatcctg attttagcag gaattattgc tgaaattgct ggttttggct ggttcaaaaa
    14281 tttctttgaa ggagtgttct aaagatgaaa agcaaaatac ctcaattact tgctttcctc
    14341 tccggtttgg ttatacttgt agctgctttt atccctcaca caccctttgg aatgtttgaa
    14401 gaaactctta ctaactggtt tatgattatc tccagttttg ctattctctt aggacagagt
    14461 agcttaattc aatcaaatgt agctaaaatt acgcaaaaag caccaaactg gaaatatcat
    14521 attgccacac tggtaagttt tggagtaatg ctgatttttg gtctgctctg gggaatggaa
    14581 aacacacctg gaattttggg tcagggagaa aagttagcag aaagtttagg ctcaaaacct
    14641 tttgattatc tatttgaata tgcctttatg cctctatctt ctacgatgtt ttctttgctg
    14701 gctttctata ttgcttcagc tgcttatcgt gctttcataa tgaggacctt tgaatctaat
    14761 ctgctattga ttaccgcggt aatagtgatg cttggtagaa ccagttttgc cactatttta
    14821 acgagctgga ttcctgattc cttgcatttt cttcgtttac cagaattaac cgattttatt
    14881 atgcaatatc ctaatactgc tgctcaaaga gctattctta ttagtgcagc tttaggtgtc
    14941 gtgggagctt ctttaaggat aattctggga attgaaaggt cttatttggg aggagaaaaa
    15001 tgagtgaaga tatcaatcta accaaaacct taagagaacg acgaatcatt ttccttctac
    15061 tggtattagc tgtagcaatt cctataatat ttcctttcag tttgaagaca catagcactc
    15121 ccttaacgga aaaagctttt aatctgatag aatcaactcc tgcaggttct ccagtcatca
    15181 tttcttttga ttatgaccct tcaacagtca ccgaacttca gcctatggct attgctatca
    15241 ttgagcatgc ctggcggaaa aaccatcgga ttattgctat ggctatgtgg cctcaaggtt
    15301 cacagatggc agatatggct ttctctgaag tccaaaaaaa gtttggtgaa gaaaaagtgt
    15361 atggtgtgga ttatgttaat ttaggttata aacctggcgg tatggtcatt attcagacta
    15421 tgggacgaaa cttaaagacg gttttcccca aagacactgc tatgaatgat tatgatagca
    15481 tacctctatt gaaaaatatt aagaccataa aagatataaa atatgtggtc tctttatcag
    15541 caggagaccc tggtttacga gattggataa tgactgctaa tggtaaattt ggcattccag
    15601 ttgctggagg gaccacagct gttagtgcac ctggcttctt accttatgta aatgaccaga
    15661 atcagctttc aggtttattg ggcggattga aagcagcagc agaatatgaa ttacttctgg
    15721 gttatgaagg aactgccagc agaggaatga atccccaatc catagcccat cttttgattc
    15781 tggcattaat tgtagcaggt aatattcgcg tctggagaaa tagaagaaaa gaaaaaatgg
    15841 caaaggaggt gaataatggc tgaattgatg aaaactatcg gtatctggct gagtgcttat
    15901 ttcactctca gcatcttcag ttttctttac aaagataatc cgttctataa gctggcagaa
    15961 caaattttcg ttggtctttc tgctgcttat tggctggttt acaccatata caatatctta
    16021 attcctaatc tttttaataa gcttttcact gattttgcac ataactggat tttactcata
    16081 cctatggctt tgggcattat gatgttgatg cgaatgtttc ccaaactgga atggctaagt
    16141 cattatccta tggcaattat ggtgggaact actgcagcca tagctatgct tctggctttt
    16201 caatctgatt ttctcagcca gatagcagct acgatggtca atcctttcaa atactcatct
    16261 ccagtagata ttgtaggcac tattcttctg attataggaa ctatttgtgg tatatatttc
    16321 ttctacttca gtaaaaagca ggaaggattc tataaaattc cttccaaggt tggaatctgg
    16381 tttcttatgg taagtttcgg tgcctctttt ggttataccg ttatggcacg tatttcacta
    16441 ttgattggta gattggactt cctcttacga gattggctac atctaatcag atgaaaaagc
    16501 tctctcttgc tccaagaatt taagcattat taaaactgaa ggttttaagg aaaaaggata
    16561 tggatttaaa agaacttgtc tctctttctc cagctataaa agaattgatt gataagatta
    16621 gcaatatttc ccaagagata atgagccata actgggcaga agccaatgct ggcaatcttt
    16681 ctcttaatat cagtcaggaa atgaacgaac tgaaagaaac aaactcagaa tggtatctgg
    16741 taactcgcac cagaagtcgt tatcgggaaa tggtacaaga ccctatttct tctttaattt
    16801 tgattcaatt gcaagctaat caagaaaagt gctatcctgc agaggctaaa cctacttctg
    16861 aatggtcttc tcatcgtagg cttcatcaat attttctgga agttggaaga aaagataggg
    16921 tgattatcca ctgtcaccct tcaaacacta ttttgctcag tcaactacca ttttatccaa
    16981 ataaaatccg aattaataaa atgctggcag aagctttgcc agaattgcct atttaccttc
    17041 cttctggagt gggaacagct ttttatgcac ctcctggttc ggaagcttta gctacaaact
    17101 ctctggaatc tattggtgat agcaaagctc taatctggca atctcatggt ttgctttgta
    17161 cgggaaaaga cctggatgaa gctctggatt atatggaaat tgtagaaaaa gcggcagaac
    17221 tggcaattcg taagcttttc ttaactcagt taaggttttg aacatactat ttttctcccc
    17281 tatctgaatc attttttatt gacgaataga atttaaagat taatgaagaa tagtagaata
    17341 taaagggtat atatataaaa agtagtttaa ggagtgataa atgaaacata gagttataat
    17401 cctgctatta tttattctga ctgtgagctt tctgggtgca aaaagtccag atcctaaagc
    17461 agtagtggga aaaatagata atattactta cacctatgca gaatatgata aaatcctttc
    17521 caattattac gcttattatc agaaaaaatg gggacatcca ctttctacag aagaaaaggc
    17581 acaattaaat aatcaatgct gggaagagct ggtaggacgt tatatctatg ataaagctat
    17641 aaaagcggga aaaataacca taacgcaagc agaactcctg agagaagcga aaaagaatcc
    17701 ccctgaagcg gtaaagcaaa ttcctgaatt aaaaactaat ggcaagtttg accgtaaaaa
    17761 atatgaagat gcactgaata atattcccga attcaagaaa gctgttttgg aagaagtgaa
    17821 agctacttat caatatcaaa agttactgaa tactataaga aatgaggtca cagtcagtga
    17881 ggatagcgtc tataaagaat ggttccgaga taatgacctt gtggatgctc agattatctg
    17941 ttttgatgcc aataagttga ctgatataac agccacagaa gaagaaggta gaacgtatta
    18001 cgaatcccat aaagagcagt atcgcaaaga gaactgtcgt tcttatcgtt atgtaaggat
    18061 tcccaaagcc ccttcaacag cggattcctt agccgtaaag gaagaagtga tgaagatata
    18121 tcaggattta cttgcaggtg cagattttgc agaaatggca aaagctaaat ctcaggaccc
    18181 tggttcagca gagaaaggtg gcgatttagg ttggttcggt agaggtcaaa tggttcctgt
    18241 ttttgaagaa acagctttta aaacaagtcc tggacaaata gcagagccag tactttccaa
    18301 ttatggctgg catatcattc aaactttaga ccgtcgtcag ggagaaaacg gtgaagaagt
    18361 tttagctcgt catattcttc ttcgtattga gcccagtgag accactttaa agcagctaaa
    18421 aatggatgct ttcaccttat atcaaaaggc acaggagaaa gggttgcaaa aagctgctca
    18481 agagatgggc tataaagttg aagaaagtgg agttttccag gaaaaagact catttattaa
    18541 agggatagga agagatgcca acttggttag ttttgccttc caaaatcctg tggggtcaat
    18601 tcctaatatt tactattcac ccaacggtga tgcctatatt tgtgagttat cggatagtat
    18661 ttctgtttat tatacccctt ttgaagatga aaaaagtaga attatgaaca ccgtcactaa
    18721 aaataaaaga aataattata tgaatgaata tgctcacaac ttcgttcaga actttactcc
    18781 tgatcaatat ctggaacaag cggcacagga tagtttaatg gtgatagaaa tcaccaatca
    18841 taaaaaagga gaccctataa gttctctggg aaagattccg gatttggata atgcactatt
    18901 tgaaactccc gtgggaagct ttacacctct aattaatgac cagtcacgct ggtttctggc
    18961 taaaattaac aaacatcaag ttccagatcc tgcacaatgg gaaaagaata aagccactct
    19021 tattaaagag gctaaagaaa aagcaggaca ggaacatctg aataagtggt atttagaaga
    19081 acgccgtaag gttactatta ttgataatag aagagatttc tatgacctga cttccaccga
    19141 caaggtcatt caactttagt tccgaaaaca ccatctctat atgtttgctg gggaattatc
    19201 tttaataatt ctccagcttt ttcattggtg aaagtataag cacgaatata gtggtcggat
    19261 aaaaaggttc ctctaccatt ttcaatatcc tcaagaatac ctctaagaat aatcttttca
    19321 ttaatcaatt tttgtttata cttttctttt ttctgtttcc ccagttgcaa taatttttgc
    19381 gcccgaaggt ttttctctgg tttaggaacc tgctcgggaa gagaataggc aactgtgcct
    19441 ggtctacgag aaaagggaaa aatatgaaga taggctatag agagtttaga tataaagttt
    19501 agcgtggtct ggaaatcgga attatcttca cctgggaaac ctgttataac atccagtccg
    19561 atggctgcat caggaaaaaa actaaggata ttattcagta aagcttcaaa atctctggta
    19621 ttataaggtc tgcccatatt ttttaagata cggtcacttc ctgattgaag agggatatga
    19681 aagtggggac agagttttgt acattgtctc aatcttttta taagctccag agaaaaaaga
    19741 tgcggctcca aagaacttaa tcttattagc tctaaacctt caagttcctc taaggcttct
    19801 actacatcag ccagattttt cccctcatct tcatacaatc ccagattgat tccacccagc
    19861 actatctctt tatatccatt ttccacaaac attcttgcct gagtgatgac ctctgaaaat
    19921 ttagcagaac gagcaggacc acgagcatac ggaacggcac aataataaca acgaaaatca
    19981 cagccatcct gtattttctg aaaagcgcgg gtatgctcta tcatcttttg cactggttta
    20041 aaattaaacc tgtctactgt cattatatcc tgaaaatgat aatcagcatt atccaggagt
    20101 tctgccaggt ttagtttgtt ttgattatct acaatgaggt caatatctcc cagttttttt
    20161 atctcatcaa aatttctttg tgcatagcaa ccggtaacaa taactttcac agctggattt
    20221 ttatttttct gttttagcgc cttacgaatc agattacgac tcttataatc tgtcctttga
    20281 gttacggtgc aggtattgat gatatagata tctgcatcac tatcaaaagg aaccagagtg
    20341 tacttctcag gattaaactg tccagaaata atagcagatt cagcggtatt ggttttacaa
    20401 ccgagtgtgg caatggcaat cttaatcatt tatatttctt aataagtggt aaagaaggaa
    20461 accagaaaga tatcatttct ctggtgcata atatttacat aaaaactgat agataactgg
    20521 agcataggtt tgatgctcaa tttccagcac ttttgcggct atttcttcgg gacttttaca
    20581 atcggatata tccactttta cctgagctac aattggtcca ttatcatatt gaggatccac
    20641 cagatgaaca gtcactcctg ataccttatc accagaggca aatactgcat tatgaacatt
    20701 cataccatac ataccttttc caccatattt gggtaaaagt gcaggatgga tattgagtac
    20761 cggaatcttc actttttcca gaaaagagga ggatagcaaa ttcataaaac cagcgagggc
    20821 aataagttca attttctctt gctgacagag ttccaacagc tgattttcaa agtctttatc
    20881 ttttttggga gaaaggatgt agcaaggaat attcagtttc tcacataatt gcaccactgg
    20941 tgcatctttg cgggaagcgg tggcaaatgc aatctctaca ggaagagaat gttttttaaa
    21001 atattcatag agggcttgaa gattggaacc tcttccagca ccactggtta aaactgctat
    21061 tcgccaggtt ttagatggag ttctttccat tgttcttccg aaacttccga gggtgctcca
    21121 cacatcaaat cagtagcttt caaagtctta ggaaaagcaa taacatctct gatagattct
    21181 gctctcgtca ttatcatcac taaacggtcc aatccaggtg caattcctcc gtgaggtgga
    21241 gtgccatatt ttaaagcttc caggaaaaat ccgaatcttt cattgagttc ttgttcactt
    21301 aatccgagaa tatcaaaaat ccttctctgg aggtcatagc gatgacaacg aatacttcca
    21361 gaggaaagct ccactccatt gcagactaaa tcgtagagct gacctataat ttgaccatat
    21421 ttatcaggtt catagagcca gggaatatgt tcttctctgg gtaaggtaaa catatgatga
    21481 gcgggttccc atcgtttttc ttccgcatta tactggaaaa gtgggaaatc tgttatccag
    21541 acaaagcaaa aagtattatc agggataaga ttttgccatt cagctatggt atttctgaga
    21601 gcggcaagaa ccttggaagt catctcgtag ttatctgcta cgatagcaat taagtctccg
    21661 ggattagctt gagtgagttc aattatagcc ttgctttctt cttctgttaa aaatttactt
    21721 atcccagatt ccaatccctt ttctgtcact ttagcaaaag cgattccctt tccaccctga
    21781 tgttttgcca ctgagattaa ttcgtcctgt ttggaacgac tgtagtctgc accatcagga
    21841 atgcatagag ctttaatcac cgctccttgc tgaatagcgg aacggaatac ttgaaattga
    21901 gtgttttgca gagcagaggt aaggtcactt aattccagag caaaccgcaa atcaggttta
    21961 tcacagccaa aacggtccat tgcttctcta taggtcaaac gagtaaaagg aatggggagt
    22021 tctatattca acacctccga aaataaagtg tgcatcaatc tttctaacaa atcaaagatt
    22081 tgttcttgcg tcacaaagct catttcaata tccagttgcg tgaattctgg ttgtctatca
    22141 gctctcaaat cctcatcccg aaaacaacga gcaagctgaa aatagcggtc aaatccagca
    22201 atcatcaaaa gctgtttaaa catctgagga gattgaggca gagcataaaa tttacctgga
    22261 taaatcctgc tgggcaccag ataatctctg gcaccttcgg gtgtactttt catcaggata
    22321 ggagtctcaa tctcatagaa accttcttga gaaagaaagt ttcgcatagt ctgcataatc
    22381 tggtgtcgca tcagaatatt tttttgtaga gagggtctgc gtaaatccag atagcgatat
    22441 tttaatctga ggtcttcttc tgccagggca gcttcattca gctggatagg taaaggttca
    22501 caatcattga gaagatatat ttcttctgct ttaatctcta tatttccggt gggaatattc
    22561 atatttacat tttccggagt gcgagctacc actttccctt ttgctgcaac cacatattca
    22621 agtcgtaatc gttccgcttt ggaaaaagac tctttctctt ccggatgaat taccacttga
    22681 atcattcctt taatatctct caaatcaata aagattaaac cacccagatc tcttctgcta
    22741 ttaacccatc ccattaaagt cacatcttta ccaatatctt tagacctcaa atcaccacaa
    22801 taatctgtcc tcttcaaatt acccagattg tctatcatca taaactcacc ttcaaaagtt
    22861 atttaaatac atattcttga ccgaatttaa tgcctctttc cagcttaacc tggtgaagca
    22921 ttatccagcc tgctataaaa aacactatta aagatataat tgcatatcgc tgatttcctg
    22981 ctaaacgagt tatttcacca taaacaaaag gacctataat agcagatatt cttcccacca
    23041 gcgtataaaa tccaaagaat tctgcttgct tatccagtgg agttagcaag gagagcatag
    23101 tcctgctatt agcttggctg cttccaatag ccagaccagc cagaagtcca aggtagtaat
    23161 attcctgtgc agtattacag aaaaatgccc agaccactac caccacccat attattaaag
    23221 atatacttaa agctaaacga acattgagtt tatccgctat caaaccaaaa agtgcagctc
    23281 caacaaagga agttacttgt gcaataatga aaaagatgat gagactctgt tgcgtcattc
    23341 caaattgtgc tccaccaaaa atagcagcat agacgataac ggtgtagatt cctatattat
    23401 agagaaaata actgattaaa aaattgagta aatcgggcat cttttttatg tgcttaaagg
    23461 aaaacaacac ccgatccagt gccactttaa aatagttctt tttttgcact ggatactttt
    23521 tttcctttaa ggtcaaaaaa gttatgctgg aaaaaattaa tatatgcaaa gccaccatag
    23581 gccagacaag ctttatgtgt ttatctatca atgccaatgc taaaatgaga gttactaatc
    23641 ctccgatata gccaaaaccc caacctaaac ctgatatcct tccgaggtct tttttcattc
    23701 ctacttctgg taaaaatgca tcataaaaga cattggcact attaaaagaa aagttggcta
    23761 tgataaaaag tatcattgcc agaattaaat ctccctgacc aacaaagtaa agtagtgcgg
    23821 tggaaataat agttaaaata gtgaagaaaa ggaggaatct ctttttggaa cgagaataat
    23881 ctgccatagc accgagaata ggagcaaaga tagcagctaa agtcattgag atagctattg
    23941 cttgagacca taaccattca ccaccttcag gtcttccaga agctatggtt tgcacaaaat
    24001 atacactgaa gacgactgta actatgaccg tggtaaatgc cgaattggca aaatcataga
    24061 acatccagcc gatagcctct tttcttagtt tcattgcttg ttttcaggta aactcaatct
    24121 ggtaagttcg ttccattcac tggttatttg ttcattggga ctggctataa aaatctgata
    24181 gtgaaaatca aggaactctt tgatgcgatg cgtgtgcagc tggtctaatt ctgcaaaaac
    24241 atcatcaaaa agcataatag gttttataga ggttaaattc tctatcaggg aagcttgaat
    24301 cagctttaaa ataattacgg tgattctttt ttgaccctga gaaccgtagc tcttaagatt
    24361 attttgattg agcagaaatt catagtcatc caaatgaggt ccaaccagcg ttcgttgata
    24421 atttttctcc ctcttttcca gttcctctaa tatccttaaa agcttatcta tactatttgg
    24481 ataatcctct attccattaa ctttatactg tatagatata tctttaacct gttcagagat
    24541 atgaggatat tgttccttta aagcctgatt gacgagattc agatattttt gcctgtattg
    24601 cagaacaggg aataagaatt cacagaaatg gaggtcccag ctatgttttt cagcataagt
    24661 atattctttt ttaagcaggt tattgcgttg ctccacaata tgtaaataat tccttaaaag
    24721 acttatgtat tcaggataaa tttgagagag agcaaggtca aaatattgtc tgcgaaaacg
    24781 aggtgagcca tcaattagat tcatatcttc tggtgcggaa tagattactt tcacgccttc
    24841 atagattcta ctgagttgcc gaactggatt tccattgatt tttaataact tcttcccttt
    24901 ttgccaggat atctgtatct ccaaaccgag ttcattatca gtttggaaaa gaccttttat
    24961 cgaaaaaaaa tcataaccct gctcacagag aaattcatct ctatgaaaat gcaccgattt
    25021 acctatccca caataagcta tagcttccaa tagattggtc ttaccatagc cattgggtgc
    25081 gattatcaaa ttaccagcag gatgcaaatc aaatctggca ttctcataat tgcgaaaatg
    25141 caccagaaag agctgtttta gaatcaataa atcaggtccg caaaggcatc aacaaaaagg
    25201 ttattttctg attcttgagt ggaggctcat taaataccat tatcgggtct ctggaatcac
    25261 ctaattttat caagacctgc tctgtatcta ttgcatccag tatggaaagc aaatatttat
    25321 tattaaagga aatagaggtc ggaactccat tgtattcata tttatctata aattctttcg
    25381 cctcacccgt ttctttattg acggtgctga tttcaaaatg gtctttatcc acatcaaatt
    25441 tgaccctgcc actatcttct ggcgctaata atgctattct gcgtatggat tgcattaaag
    25501 tttccttatc cagaataaga tgattgggaa gctctgctaa aaaggctttt tcatattccg
    25561 gatagttatg gtcaatgata ttggaaacta ccaggtaatc agcatagccg aagatgatat
    25621 tatttctctc aaaaatgacc ttcatttcct tgaccgcggg atcgtgtatc ttctgcaaga
    25681 aattcaatgt ctttacagga ataatcttct ctaataactg tcccgaatag ggatcatcaa
    25741 agatggattc atcaatattt tcttcaccac tttctgtgac tgaagcagct gtgttcggaa
    25801 caattatttc tgccaccttt cttccatctg ttgctgccat taaattaact ttttccataa
    25861 ttttccaaca aactccagta aggaccggtc tattgacatc agtagagaca gcaaaaacgg
    25921 tcttgctaat catcctatta aaggtctctg cattaaccag agtagcgttg tcaaaactgg
    25981 gcattagaag ctgaggaaag agagtgtaat cagcacaaag aatattgaat tcaatcttgt
    26041 tacattgaat catcaagagg tcttcatttt gccagatatt gattacagaa tccggcatct
    26101 gagatattat actattaaaa taacttgctg atacagcaat aattccacta tcactcacag
    26161 ctgcatttaa aactactact gcagtaattt ttaaatccgt agcagtaatc ttaacttcac
    26221 cttttttctc atctgcctct attaaataat tagtcaaaat tggagaagca gctttcgttg
    26281 atataatccc tgctaaatgc tgaatgtgct gaactaattc acttttttca actgcgaatt
    26341 tcataactac ccctttatta agatattact ctattaattc cttaaaattt ctttttgcca
    26401 ccttgtcaat aagtttctct gaatttatac aggtatg
//
LOCUS       DAORZF010000022        25948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_1593_length_25948_cov_390.963079, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000022 DAORZF010000000
VERSION     DAORZF010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 25948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25948
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1593_length_25948_cov_390.963079"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PK015_03870"
     CDS             <1..195
                     /locus_tag="PK015_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HOL33641.1"
                     /translation="GSIDDLVLIVLGLNMILNDIDQQILEDNWSGEGEVLHWMQRITA
                     EAENFLDRNILQKIKNFLKL"
     gene            202..813
                     /gene="rdgB"
                     /locus_tag="PK015_03875"
     CDS             202..813
                     /gene="rdgB"
                     /locus_tag="PK015_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOL33642.1"
                     /translation="MFTLLLASHNQDKAKELKALLSGMNILLLTLNDFPNIVPTIEDQ
                     DTISANAMKKAFEASLKTGLVSLADDTGLFIKALNDKPGVYSARFAGEHCSYKDNRMK
                     VLKLMEGITNRYAEFRTCVALSAPDGIISFKIGILEGKITTEERGTNGFGYDSIFMPL
                     SFNKTYAEMTDTEKNSISHRAKAIEAIIPIIKELIESSKGGLQ"
     gene            810..2162
                     /locus_tag="PK015_03880"
     CDS             810..2162
                     /locus_tag="PK015_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOL33643.1"
                     /translation="MIKPEVFRQYDIRGIVGEDIDEESFYLIGKGFGTYLHQKGLSSL
                     VLGGDARLTTPSLKEAFTRGALETGCTVIDLGMVATPVLYFSIWKLKTDGGAMITASH
                     NPSNYNGCKLNLGLDSVYADELQKVLHIIQKGEFASGSGTYTINTSVNDDYKDYIISG
                     ITLERPVKVVIDAGNGVGGLYLPEILENLGCEVIKMYCEPDGSFPNHHPDPTIEKNMQ
                     DLSKKVVSEGYELGIGLDGDADRIGVVDEKGKLLFGDQILNILARDYLKHNPGKSVIV
                     DVKCSKNLYDDIIKYGGVPIMYKTGHANIKMKMKELGVEFAGEMSGHIFLADRYLGYD
                     DAIYVSCRFIEVVSKTDAPVSTFLADQPKMYNTPELHIACSDDRKFEVVAKVCDELKA
                     EGYDVLDIDGARVNFPDGWGLVRASNTTPVLVTRFEAVTEERMKEIQQLIESKIYKYL
                     "
     gene            complement(2223..3494)
                     /gene="hisS"
                     /locus_tag="PK015_03885"
     CDS             complement(2223..3494)
                     /gene="hisS"
                     /locus_tag="PK015_03885"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOL33644.1"
                     /translation="MQYSIPRGTYDILPEDSPKWHKLIAEFRKLVEAYGYAEIVTPIF
                     EQAALFERSSGESSDVVKKEMYRFTDRKGREFALRPEGTAPVARSYLENRMDIGSSYK
                     KLYYLGPMFRYDRPQAGRYRQFYQYGVEFIGSNNPYYDAELIAILWNYLTALGLKKIR
                     LELNSVGCSSCAKEYDKALQSYFQPYLNQLCPDCQERFERKPRRILDCKVKECKDIAK
                     NAPIQLDYLDEPCKEHFSEVQHYLNLMNIPFVINPRIVRGLDYYTNTAFEVIYEGIGA
                     QNALAGGGRYNGLLEQIGGKNIPAVGFAGGFERLLLSLNEEKITLPSSPFPEVFIVTI
                     GREAQELIIPYLNVIRREGIYAEYEPDRASLKAQLKAANNYRAHYALIIGETEVKQKS
                     AMLKNLESGEQKLYPLQPISNLIKAIQNKEL"
     gene            complement(3505..4287)
                     /gene="truA"
                     /locus_tag="PK015_03890"
     CDS             complement(3505..4287)
                     /gene="truA"
                     /locus_tag="PK015_03890"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOL33645.1"
                     /translation="MSRFLIRVMYDGTNFRGWQTQTSGRSVQGELISVLKDLGITHPN
                     VTGSGRTDAGVHSIANYAHFDYNGRMTTQQLLLALNSKLDSDIMITGIWQVKDSFNAR
                     YSATSRTYIYLLARERTPLNRLYTGYIPGKEIVMEDLYAMSEVLKGNHNFTSLSKNNP
                     AITNHICNVSSFTIKEMKGKIVFKITADRFLHHMVRRMVGTMVNLSQKGLGVEAMKQI
                     LEEQNARQKLVLTAPAQGLYLYNVHYPDLTIEDYPYNHFLNN"
     gene            complement(4294..4935)
                     /locus_tag="PK015_03895"
     CDS             complement(4294..4935)
                     /locus_tag="PK015_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33646.1"
                     /translation="MGKRLSVLSEQHLGLRLLILVLALIAAISASWQKILLLIGIFLS
                     FMLLDSSLYKQVLKGLRVTLPFFTAYWIFGTIFKVQFPLMALFTLKVIFFILAGVYAL
                     GNLNLGKVLYDTRFIRKYKWGRWLVHFGLATSFYIQAYTAYFTNHKPEGRNTIGEVID
                     SMFEAGTSVLQKSGEIEEKLNLSIDSDQTHSSSTVSNMIALLLSVIMIVIIIW"
     gene            complement(4935..7133)
                     /locus_tag="PK015_03900"
     CDS             complement(4935..7133)
                     /locus_tag="PK015_03900"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOL33647.1"
                     /translation="METNTLYNARKYYKSAQSRPLNANGRPTNQAVEEYTKAIKKCGI
                     ILSRPNPGKEADDALFIMACALYYKGNSAFQAKDQFEALINNFPESPYWGEAHIYLAK
                     VYREINQKEQANKILEEFILDPSQKKLHPRALLTLAEFEIEDKNYIKAQYWLEKLITN
                     YPKSKEYREAFFTFGKNYFIQKDYAKSLREFEKIARDPRIPQSLKLEARYYIALNQFY
                     LGDYEASWKTLLKLLKDENRPDKIAQARVLKARLLIAQGDTEQGIAEVADITKSYPRT
                     QSSAEANYYLGEYYFYQAGDLTKAMTAYNKVRTEFPNSELATIAQKKASAIDFLKNKD
                     KLDPEQKLQELIDYYMTAAEYYLNQFALPDSAILMYQKLIDTRDIITARKDSLLNVIS
                     YQQSAIDSISVLIETLPAISEEDSLNLSFSQAETALGDSLIASDSLTIADNTNLSTDS
                     DSLKIEEEIVNPAEQRKKLEQSLTTLHAELKNNQDRLAALDEILDRFEREIIPLALFS
                     QACVYQKIPGGNEQMQALYNRMQEEYPANKYTRALENMLAGKPIRLIDPREEEQYLLL
                     DKALGYAETYPDSMLIILNELVNSDYPEIKLKANFRLGWYYTFEARDTTAAKPYLAKV
                     LEYDSDTEYTTITAKFFDGKNFQLNRFDQLLLSFTESDSLREELSSESEAQDTTIVPE
                     EESILSVPEKKEETETINMSKNPENNFFPQPESPGEQGKKNPFFIPNEKP"
     gene            complement(7216..8745)
                     /gene="hutH"
                     /locus_tag="PK015_03905"
     CDS             complement(7216..8745)
                     /gene="hutH"
                     /locus_tag="PK015_03905"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HOL33648.1"
                     /translation="MSTIYIDGNSLNLEKIEEVAYKRTPIALTEACIQQVNKCRDYVE
                     KIIDKGELVYGLNTGFGKLSSIAIPRDNIAELQVNLIRSHSTSVGPAYTVEQTRAIML
                     LRINVLAKGHSGIRLETLQTLVDMLNKGVHSIIPMRGSVGASGDLSPLSHLALTLIGE
                     GEAEFEGEIISGAEAMQRAGIKPVQLQAKEGLALNNGTQVMTALGVLNLLEAERLCKY
                     ADIIAACTIDAMKGTPSAFDSLVHKLRPHKGQIASASNISNLMNNSPLRESHINCENV
                     QDPYSLRCTPQVHGAVRDAIDYVRGVLEVEINSATDNPLIFPEEEKVISGGNFHGEPL
                     AICLDTLALAISELASISERRIEQMLNPALNRGLTPFLAKNPGIDSGYMIVQLTAASL
                     VSENRVLAHPACVDNIPTSANQEDFVSMGSVSANKLIQVVENVRTVLAIELMVAAQAL
                     DQRNIPSSSVLEKVKSSFRQIVPTLQEDRITYPDINAAVNFLKKSTLLELVQTEGINL
                     Y"
     gene            9487..9819
                     /gene="rpsJ"
                     /locus_tag="PK015_03910"
     CDS             9487..9819
                     /gene="rpsJ"
                     /locus_tag="PK015_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOL33649.1"
                     /translation="MSKGNFENRIRIKLKAYDHRLLDQSVAEIVKSTRNTGAKVVGPI
                     PLPTDRTIYTVLRSPHVDKKSQDQFQMLVHKRLIDILNPTQQTTNALKKLSLPAGVHV
                     EIKANTRS"
     gene            9822..10454
                     /gene="rplC"
                     /locus_tag="PK015_03915"
     CDS             9822..10454
                     /gene="rplC"
                     /locus_tag="PK015_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOL33650.1"
                     /translation="MLGLIGKKIGMTQIFDPDGKVIPVTVIQAGPCRVVYKRSKAKEG
                     YDALQLGYEEIEEKRSNRPKIGHCKKNGSPVYRYLREFRPSKSQNIEDIEVGTEFKAD
                     IFQPNEKVSISSRSKGRGFTGVMKRHGFAGFMSSHGVHESFRGPGSIGQCAQPSRVFK
                     GLKMAGHHGNQKVTVHHLKVVKVDPEQNLIMVKGAVPGHRNSLVIIHKES"
     gene            10464..11099
                     /gene="rplD"
                     /locus_tag="PK015_03920"
     CDS             10464..11099
                     /gene="rplD"
                     /locus_tag="PK015_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOL33651.1"
                     /translation="MATAIKFNSIGEKIDEIELPAQIFEVDVNSPKVLLNEVVTQYLL
                     NQRQGTVQKKNRAMTAGSTRKIYRQKGTGRARQGSIRNPVRVHGGRAFAILPKNWYRP
                     IPKKKKRLALKVALTDRARDGRIFIIESFDFSEPNTKQAREILNKIVPDKGWKLVVTD
                     GHHFPTVKSFNNLPDVMTDRADSLYAYEILKSHYIVMTQDALNKVEEVFSK"
     gene            11096..11383
                     /gene="rplW"
                     /locus_tag="PK015_03925"
     CDS             11096..11383
                     /gene="rplW"
                     /locus_tag="PK015_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOL33652.1"
                     /translation="MIHPRNIIISPIITEKSEKLARDNNMYTFKVSINANKIEIKKAI
                     EKIFSVKVLDVNTIRMLGKPKSLGKYNGKRPDWKKAVVTLREGDKIADFEV"
     gene            11394..12224
                     /gene="rplB"
                     /locus_tag="PK015_03930"
     CDS             11394..12224
                     /gene="rplB"
                     /locus_tag="PK015_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOL33653.1"
                     /translation="MGIKKYRPITPSLRFRTGYTFEELTKKKPEKSLIKPVRKTAGRN
                     NSGRITCRHRGGGHRRHYRIIDFKRDKFGIPAKVAAIEYDPNRTARIALLHYIDGEKR
                     YILAPEGLQVGTKVMSGPEAEIAIGNAVPLERIPLGATVHNIELKRGKGGQIARSAGT
                     FGQVVAKDGDYVHVKMPSNDVHLIRKECLATIGQVSNIEHNLVHIGKAGRNRWKGIRP
                     TVRGVAMNPIDHPMGGGEGKSSGGRHPVSPWGKLAKGGKTRKPHKYSDKYIVKAVKKK
                     "
     gene            12237..12512
                     /gene="rpsS"
                     /locus_tag="PK015_03935"
     CDS             12237..12512
                     /gene="rpsS"
                     /locus_tag="PK015_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOL33654.1"
                     /translation="MARSIKKGPFVDAHLLKKVEALNAENKKAVIKTWSRRSMIIPDF
                     IGHTFSVHNGHKFVPVYITENMVGHKLGEFSPTRTYRGHKEKKTKAK"
     gene            12526..12867
                     /gene="rplV"
                     /locus_tag="PK015_03940"
     CDS             12526..12867
                     /gene="rplV"
                     /locus_tag="PK015_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOL33655.1"
                     /translation="MEATAKLRFARGSARKARLVLDLIRYKPVPEAQNILRFSRRAAA
                     VPISKLLDSAIANAKLQDPKLDLNKMVVSVATADEGPQMKRYMPRAMGRAYMIRKQTC
                     HIALEIRTLED"
     gene            12878..13534
                     /gene="rpsC"
                     /locus_tag="PK015_03945"
     CDS             12878..13534
                     /gene="rpsC"
                     /locus_tag="PK015_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOL33656.1"
                     /translation="MGQKIHPILYRLGVNKDTESIWFADGQTYVDYLQEDIRIRKYIH
                     KRLEDKMVSRVQIWRKTNSITIDIHTARPGLVIGKKGEDIERLRNELNVLINKNRKSP
                     IAVSINIEQIDKLWLDARLVGREVARQLEERVPFRRAMKSAIRNVMKEGAQGVKVQVS
                     GRLGGAEIARTETYKQGRTPLHTIRADIDYACVEALTTYGVIGIKVWIYKGDILGEEK
                     "
     gene            13535..13954
                     /gene="rplP"
                     /locus_tag="PK015_03950"
     CDS             13535..13954
                     /gene="rplP"
                     /locus_tag="PK015_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOL33657.1"
                     /translation="MLAPKKVKHRKVMKGRRKGLAWRGNTVAFGDYGLMALEDAFITS
                     RQIEAARIAITRYMKRAGKVWIRIFPDKPITKKPAETRMGKGKGSPEYWVAVVRPGRV
                     MFEIEGVSPEEAKEAMRLASHKLPIKTRIIAREGIEL"
     gene            13951..14145
                     /gene="rpmC"
                     /locus_tag="PK015_03955"
     CDS             13951..14145
                     /gene="rpmC"
                     /locus_tag="PK015_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOL33658.1"
                     /translation="MKMEEMREMTNEELQTRLEELRIELFNLRFQKSKNLLDRQDRPR
                     LVRRDIARILTLLKEREKNQ"
     gene            14153..14407
                     /gene="rpsQ"
                     /locus_tag="PK015_03960"
     CDS             14153..14407
                     /gene="rpsQ"
                     /locus_tag="PK015_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOL33659.1"
                     /translation="MQNTHKMIKQGVVVSDKNDKTIVVSVQRQFIHPLYKKTVRRHKK
                     FMAHDEHNEAHEGDIVQIIEHRPMSARKRWALYKIVERSK"
     gene            14420..14788
                     /gene="rplN"
                     /locus_tag="PK015_03965"
     CDS             14420..14788
                     /gene="rplN"
                     /locus_tag="PK015_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOL33660.1"
                     /translation="MIQVQTMLNIADNSGARKAMCIKVLGGTRRKYATVGDEIVVAVK
                     SATPGGKVKKGSVEHAVIVRTAKEIRRPDGSYIRFSDNAAVIIDERHDPKGTRIFGPV
                     ARELREHHYMKIVSLAPEVL"
     gene            14831..15136
                     /gene="rplX"
                     /locus_tag="PK015_03970"
     CDS             14831..15136
                     /gene="rplX"
                     /locus_tag="PK015_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOL33661.1"
                     /translation="MRLKKGDYVVVISGDDKGKKGHILKAYPKTGKVIVEKVHMIKKH
                     TKPSQNNPQGGIISMEAPIDASNVMLFNDRLNTVSKPVYKVREGRRVRICKKSGDEL"
     gene            15148..15693
                     /gene="rplE"
                     /locus_tag="PK015_03975"
     CDS             15148..15693
                     /gene="rplE"
                     /locus_tag="PK015_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOL33662.1"
                     /translation="MNRLKEHYKNTVIPNLQKQFNYKNVHQVPKLDKIVVSMGVGAAT
                     QNKAILDNAVKDMEQITGRKPIVTRAKKSISNFKLRKGMPIGCKVTLRDEIMYEFFDR
                     LVSIVIPRIRDFHGIPTDSFDGRGNFSIGLKEQTVFPEIEYDKIDTIRGLNINIITTA
                     RTDEEGRALLKELGMPFQHKA"
     gene            15703..15888
                     /locus_tag="PK015_03980"
     CDS             15703..15888
                     /locus_tag="PK015_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOL33663.1"
                     /translation="MARKALIIKQQRTPKFKVRKYNRCKICGRPRAYMRDFGMCRLCF
                     RKYASEGQIPGITRASW"
     gene            15907..16320
                     /gene="rpsH"
                     /locus_tag="PK015_03985"
     CDS             15907..16320
                     /gene="rpsH"
                     /locus_tag="PK015_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOL33664.1"
                     /translation="MSITDPIADALTKIRNAYRAGHQQVIINHSKLTEAIVKILAEEN
                     FVNSVQVLDKDPEQKINYKRILINLRYTNDGKPVLQGLERVSKPGRRVYVRANNLPRV
                     YNNTGCAIISTSQGVMVDRDARIKRVGGEYICKVW"
     gene            16330..16902
                     /gene="rplF"
                     /locus_tag="PK015_03990"
     CDS             16330..16902
                     /gene="rplF"
                     /locus_tag="PK015_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOL33665.1"
                     /translation="MSRIGRAPIKFDQDTHIEVKDNIVSVKGKLGELQYKLMPGITVE
                     ISDGVLQVKRSNDSKDQKALHGLTRALLQNMVRGVSEGYQKNLQVIGTGYSAERIGPW
                     LKLSLGYSHDIIMEVPEGLTVETEAVPRSKTSRTDLQSIIRVKGIDKQLVGEFAAEIR
                     ACRPPENYKGKGIRYENESVIIKAGKAGTK"
     gene            16917..17288
                     /gene="rplR"
                     /locus_tag="PK015_03995"
     CDS             16917..17288
                     /gene="rplR"
                     /locus_tag="PK015_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOL33666.1"
                     /translation="MIKSSTKIKVEHRNRRRAAIRKKLRGTAERPRLVVFRSLKHIYA
                     QIVDDTAGKTLVTASTRGKDIQIPAGLKKTEQSFLVGQKLGEKALAAGITKVAFDRAG
                     YKYHGRVKALADGARKAGLDF"
     gene            17337..17819
                     /gene="rpsE"
                     /locus_tag="PK015_04000"
     CDS             17337..17819
                     /gene="rpsE"
                     /locus_tag="PK015_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOL33667.1"
                     /translation="MFETVIDTKRVAKVVKGGRNFSFTAIVVVGDRAGNVGVGNGKAN
                     EISDAIRKAKERAQKNMFKVPIIKGTIPHEIIARFGASKVMMKPASEGTGIIAGGTAR
                     AIFEAAGIQNILCKSLGSNTPCNVVKATVLGLKSMRTLSDVAKLRGKTVAEITGQEAK
                     "
     gene            17816..18001
                     /gene="rpmD"
                     /locus_tag="PK015_04005"
     CDS             17816..18001
                     /gene="rpmD"
                     /locus_tag="PK015_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOL33668.1"
                     /translation="MKIKVTQVRSTINRPEKQKLVIKSLGLGRPGRSRIHDDNPCIRG
                     MINKVSHLVKVEELKEE"
     gene            18003..18458
                     /gene="rplO"
                     /locus_tag="PK015_04010"
     CDS             18003..18458
                     /gene="rplO"
                     /locus_tag="PK015_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOL33669.1"
                     /translation="MLTLSNLEKPQGKKAKKRLGKGQGSGTGHQAGRGNKGHKARTGG
                     KIPAYFEGGQMPINRRLPKRGFKNVFHKNYRVLNLSKLQDIEETNFDITKLEMLGLIP
                     VKGKKGKYPVKVLASTTEEFTKTVHIRANAFSKKAKQLIEANGGKAEVI"
     gene            18460..19785
                     /gene="secY"
                     /locus_tag="PK015_04015"
     CDS             18460..19785
                     /gene="secY"
                     /locus_tag="PK015_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOL33670.1"
                     /translation="MFKTLANVFRIPDLRKKILITLLILTLYRLGSFIPIPGVSGTEL
                     KSFFARQTGSIFDLLNLFVGGNFERASIFALGIMPYITASIVIQLLGSIVPYFEKLRK
                     EGADGQKKLNQITRYCTVALAAFNAITVTVGLVNMHGATGPVVPQPNFLFHLTGVITL
                     VTGTMIVMWLGEQITEYGIGNGISLIIFAGIIARYPEGFINLFRTRFNSVSGVITSLL
                     AIAVMILATAAIIFVTEAVRKIPVQYAKRIVGRKVYGGQSTYIPLRVNTAGVIPIIFA
                     QSILMFPATLITLFQGGEAEVGSFWYNMRIWFSPGHWVYTIIYVALIIFFAYFYTAIV
                     LNPTEMAENMIKYGGHIPGKKPGKKTAEYISSVLTRITLPGAVFFALIALLPEIMTNV
                     FKLPFYFGGTGLIIVVGVALDTLKQIESHLIMRHYDGFMKKGKLKGRTS"
     gene            19788..20561
                     /gene="map"
                     /locus_tag="PK015_04020"
     CDS             19788..20561
                     /gene="map"
                     /locus_tag="PK015_04020"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOL33671.1"
                     /translation="MIIRKSPAEIDKIRISCQILGELLDLLAEKIMPGVSTWDLDAFA
                     YDFIIKNGAKPAFKGYQVPGLSPFPSTLCTSLNSVIVHGFPSKKVILKEGDIISIDVG
                     AIKDGYYSDAARTYAVGTISPDAQRLMEVTLNALYKGIEAAIPGNRVGDISNAIGTYV
                     ASMGYYVADDLTGHGVGRYLHEEPQIPNAGSAGHGPRLQPGMTLAIEPMVNIGTNRVK
                     ENGWEFSVADGTLSAHFEHTILITDSQPEILTVAKGERV"
     gene            20558..20776
                     /gene="infA"
                     /locus_tag="PK015_04025"
     CDS             20558..20776
                     /gene="infA"
                     /locus_tag="PK015_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOL33672.1"
                     /translation="MSKTGVIEVEGIVTEALPNTTFKVQLENGHTILAHSSGKMRMHY
                     IRILPGDKVKVELSPYDLTRGRITYRYK"
     gene            20816..20932
                     /gene="rpmJ"
                     /locus_tag="PK015_04030"
     CDS             20816..20932
                     /gene="rpmJ"
                     /locus_tag="PK015_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOL33673.1"
                     /translation="MKVRSSVKKLCKDCRIIKRNGVIRVICSSNPKHKQRQG"
     gene            20944..21312
                     /gene="rpsM"
                     /locus_tag="PK015_04035"
     CDS             20944..21312
                     /gene="rpsM"
                     /locus_tag="PK015_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOL33674.1"
                     /translation="MAHIAGIELPKNKRLFIGLTYIYGIGRSRAIEICKKANIDEMKK
                     VEDLTPEEEKLIRDIVQNEYVVEGALRTQVAMNIKRLMEIGCYRGLRHKRGLPVRGQR
                     THTNARTRKGPRPGGIRKKK"
     gene            21327..21716
                     /gene="rpsK"
                     /locus_tag="PK015_04040"
     CDS             21327..21716
                     /gene="rpsK"
                     /locus_tag="PK015_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOL33675.1"
                     /translation="MPKKTRPKKKRTRLSFDEGLIYIHSSFNNTIVTLTDRSGNVLAW
                     SSGGKIGYKGSRKATPFAAQMAASEVAKAAQDMGISRVGLIVKGPGSGRESALRAVNA
                     AGMKVTMIKDATPIPHNGCRPPKARRI"
     gene            21728..22345
                     /gene="rpsD"
                     /locus_tag="PK015_04045"
     CDS             21728..22345
                     /gene="rpsD"
                     /locus_tag="PK015_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOL33676.1"
                     /translation="MAKYTGPKAKLCRRFGENIYGPQKYDKILSKRKFPPGQHGSSMH
                     RKPSDYSLHLNEKQKLRYTYCLLERQFRRYFQIADKMQGITGDNLLKLLERRLDNTVY
                     RMGFATTRMQARQFVNHGHFMVNGKKVDIPSYLLKPGDIIEVRPKSRGIKPLVEAWNN
                     SEASSPYPWLTVDKENMRGQFVNIPNIEDIPTTVDMRLVVEYYSK"
     gene            22373..23482
                     /locus_tag="PK015_04050"
     CDS             22373..23482
                     /locus_tag="PK015_04050"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOL33677.1"
                     /translation="MLYLEPLQMPENVDYEKQTYSPTFGHFEIGPLEPGFGTTLGNSL
                     RRILLSSIQGSAVRFVRIEGLHHEFTPIPGTNSDYIDLILRLKQLVIYCTSIDEVTVT
                     LEHKGKGIITAAKIQEHPNVKIINKDLYLLEVTEDVDFKMDLIIGNGRGYVPADRQNT
                     EGKPLGFIPIDSIYSPVLKVNFSVAHQRVKERMNYDKLLIDIYTNGAIEPSIALFLSA
                     KLLRDMAGKICLFETEPQYIRDVYIDPELEEKERILSLPVKDIELSVRAANCLEAANI
                     KTIGELVSKTEQEMLKYRNFGKKSLEEIVQKLKKYDLHLGMDVEGIYRQIREARSRGI
                     KTEETAPNKEEQEEKSVTPPRKPVPTTKKKVKNAT"
     gene            23472..23879
                     /gene="rplQ"
                     /locus_tag="PK015_04055"
     CDS             23472..23879
                     /gene="rplQ"
                     /locus_tag="PK015_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOL33678.1"
                     /translation="MRHRVEGRKFGRETDQRRLMMNNLVKSMIEHGQITTTLAKAKEM
                     RRYVERVVTYGKNDTVHSRRLAYKVLGDRTLVKKLFTEIAPAFQDRNGGYTRIIKAGY
                     RRGDSAPMAVIQFVESSTITPKKEHIKAKDLNK"
     gene            24634..24870
                     /locus_tag="PK015_04060"
     CDS             24634..24870
                     /locus_tag="PK015_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33679.1"
                     /translation="MNTLKKIFLFPLTKNEKSFKYNLIKLGDRMMKKIILFLMVLSFI
                     LPFSAVYFKIGDYYTPGNANYVAMSDNIVYVAAN"
     gene            24919..>25948
                     /locus_tag="PK015_04065"
     CDS             24919..>25948
                     /locus_tag="PK015_04065"
                     /inference="COORDINATES: protein motif:HMM:NF019912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33680.1"
                     /translation="MGSYDTPGEALSVTVSNNIAYVADKESGLQIIDVSNPQNPFLLG
                     SYDTPGYALSVFVSNNIAYVTDDYAGLLIIDVSNPQNPAFLGSYDTPDWAESVTVSNN
                     IAYVAAWDAGLQIIDVSNPQNPALLGSYNTPGSAYSVFVSINIAYVAAWDAGLQIIDV
                     SNPQNPVLIGSYDTPSYALSITVSNNIAYVADYWAGLQIIDVTNPQNPALLGSYDTPG
                     TALSVFVSNNIAYVADVSPGLQIIDFLYAHGYYHRFPDAAAWDAGLQIIDVSNPQNPA
                     LLGSYDTPSYAYSVFVSNNIAYVAAWDAGLQIIDVSNPQNPFILGSYHTPYYAYSVFV
                     SNNIAYVAD"
BASE COUNT         8331 a         4771 c         5184 g         7662 t
ORIGIN      
        1 ggttctattg atgatttagt gcttatcgta ctgggtttga atatgatact taatgatata
       61 gaccagcaaa ttctggaaga taactggagt ggagaaggtg aagttttaca ttggatgcaa
      121 aggattactg ctgaagcaga gaatttttta gaccgaaata ttcttcagaa aataaagaac
      181 tttttgaaac tataagagat tatgtttact ctacttcttg catcccataa tcaagataag
      241 gctaaggagt tgaaagcttt attaagtgga atgaatattc tgcttttaac tcttaatgat
      301 tttcccaata ttgttcctac tattgaagat caagatacta tttccgccaa tgcaatgaaa
      361 aaggcatttg aagcttcgct taagactggt ttagtaagtt tagcagatga caccggttta
      421 tttattaaag cattaaatga taagccagga gtatattcag ctcgctttgc gggtgaacac
      481 tgttcctata aagataacag aatgaaagtg ttaaaattga tggaagggat aaccaatcgt
      541 tatgcagaat ttcgcacttg tgttgctcta tcagcacctg atggcattat cagctttaaa
      601 ataggaatcc tggaaggtaa aataactaca gaggaaagag gaactaatgg tttcggttat
      661 gattctatct ttatgccttt atcatttaat aagacctatg cagaaatgac cgatacagaa
      721 aagaactcca tctctcaccg ggcaaaagca atagaagcta ttataccaat tatcaaagaa
      781 ctaattgaat catcaaaagg aggtttgcaa tgataaaacc agaagttttc cgccaatatg
      841 atattcgtgg catagtggga gaagatattg atgaagaatc tttttattta atcggtaaag
      901 gttttggcac ctatctccat cagaaaggtc tatcttcttt agttttaggt ggagatgctc
      961 gtttaaccac cccttctctc aaagaagcct ttacccgagg agctctggaa acaggatgca
     1021 ctgttattga tttaggaatg gtggcaactc ccgtcctcta tttctccatc tggaaactga
     1081 agactgatgg tggtgcgatg attactgcca gtcacaatcc ttccaactac aatggctgta
     1141 aactgaatct gggattggat tctgtctacg cagatgaact tcagaaggtg ctacatatta
     1201 ttcagaaagg tgaatttgct tctggaagcg gcacttatac tataaatact tcagtaaacg
     1261 atgattataa agattatata atctcaggaa taacccttga acgcccggtt aaagtagtaa
     1321 ttgatgcagg aaatggagtg ggaggattat atcttccgga aatactggaa aatttaggct
     1381 gtgaagtaat aaaaatgtat tgtgaacctg atggttcttt ccctaatcat catcctgatc
     1441 ccaccataga gaaaaatatg caagacctat ctaaaaaggt ggtctcagaa ggttatgaac
     1501 tgggaatagg tctggatgga gacgctgata gaattggtgt agtagatgaa aaaggtaaat
     1561 tactatttgg agaccagatt ctcaatattc ttgcaagaga ttatctcaag cacaatccag
     1621 gaaaatccgt catcgttgat gtcaaatgct caaaaaacct ctatgacgat attataaaat
     1681 atggtggtgt tcctattatg tataaaacgg gacatgccaa tatcaaaatg aagatgaaag
     1741 aactgggggt agaatttgca ggtgaaatga gtggtcatat tttcctcgca gatagatacc
     1801 tgggttatga tgatgctatc tatgtaagct gcagattcat tgaagtggta tccaaaactg
     1861 atgcaccggt tagcactttc ctggcagacc aacctaagat gtacaatact cctgaattgc
     1921 atattgcatg ctcagatgac cgaaaatttg aagtggtagc aaaagtctgt gatgaattaa
     1981 aagctgaagg ttatgatgtt cttgatatag atggtgcacg agtcaatttc cctgatggct
     2041 ggggtttagt tcgtgcttct aataccacac cagttctggt aaccaggttt gaagcggtaa
     2101 ctgaagaaag gatgaaggaa atacagcaac tgatagaaag taaaatctat aaatatctat
     2161 aaaatataaa gaggcagact tcagcctaac ttttagagcc tgaaatctgc cttattgttt
     2221 ttttataact ctttattttg gatagctttg attaaatttg aaattggttg tagcggataa
     2281 agtttttgtt cgcccgattc cagatttttg agcattgcac ttttttgttt aacctctgtt
     2341 tctccaatga ttaaagcata atgcgctcgg tagttgttgg ctgctttcaa ttgagcttta
     2401 agtgaagcac ggtctggttc atattctgca tagattcctt ccctccttat tacattgaga
     2461 taaggaataa tcagttcctg tgcttcccta ccgatagtta caataaaaac ttcaggaaag
     2521 ggggaacttg gcaaagttat cttttcttca ttaagcgata ataataaacg ttcaaaacct
     2581 ccagcaaaac caactgcagg gatgtttttt ccacctatct gctccaataa gccattgtat
     2641 cttcctccac ctgctaaggc attctgagct ccaattcctt cataaataac ttcaaacgct
     2701 gtattagtgt aataatccaa tcctctaacg atgcgtggat tgattacaaa aggtatattc
     2761 atcagattaa gatagtgttg cacttcggaa aaatgttctt tgcacggttc atccagataa
     2821 tctaactgga taggtgcatt tttcgcaata tccttacatt ctttcacttt acaatcaagg
     2881 atacgacgcg gttttcgttc aaagcgttct tgacaatcag gacaaagctg attcaaatag
     2941 ggttgaaaat agctttgtaa tgctttatca tattctttag cacagcttga acaaccaaca
     3001 ctatttaatt ccaggcgaat tttttttaac cccagagcgg ttagataatt ccataatatt
     3061 gcaataagtt ctgcatcata atagggatta ttgctgccga taaattccac tccatactga
     3121 taaaattggc gatatcttcc cgcttgagga cggtcatagc gaaacatagg tccaagataa
     3181 taaagctttt tgtaagatga accaatatcc atccgatttt caaggtagct acgtgccacg
     3241 ggagctgttc cttctgggcg aagagcaaat tctcgtcctt tacggtctgt aaaacgatac
     3301 atttctttct ttactacatc ggaactttca ccagaacttc tttcaaaaag agctgcctgt
     3361 tcaaagatgg gagtaacgat ttctgcataa ccgtaagctt ctacaagctt ccgaaattcc
     3421 gctatcaatt tgtgccattt gggactgtct tctggtaaaa tatcataagt gccacggggt
     3481 atactatatt gcataaaaac ctcctcaatt gtttaaaaaa tggttataag gataatcttc
     3541 aattgtcagg tctggataat gaacattata aagatataaa ccctgtgctg gagcagttaa
     3601 aacaagcttt tgccgtgcat tttgctcttc aagtatttgc ttcatagcct ctactcccag
     3661 acctttttgt gataaattaa ccatcgttcc caccattcta cgaaccatat gatgaagaaa
     3721 acggtctgct gtaattttaa atactatttt acccttcatt tctttaattg tgaaagatga
     3781 gacattacag atatgattgg taatagcggg attattttta ctcaatgaag taaagttatg
     3841 atttcctttt agaacttcag acatagcata taaatcttcc attacaattt ctttccccgg
     3901 aatataaccg gtatacaatc ggttcaaagg agttctttcc cttgctaaca gatatatata
     3961 agttcgggaa gtagcagaat agcgggcatt aaaagaatct tttacctgcc agattccagt
     4021 aatcataata tctgaatcaa gtttactatt tagcgctaaa agtaactgtt gagtggtcat
     4081 ccttccatta taatcaaaat gagcataatt agcaattgaa tgaacccctg catcagtgcg
     4141 acctgaacca gtaacattag gatgagttat ccccaaatcc ttcaaaacag aaatcaattc
     4201 tccttgcaca cttcttcccg aagtctgagt ttgccatcct ctaaagtttg taccatcata
     4261 cataactctg attaaaaaac gggacataat cagctaccaa attataatca caatcataat
     4321 aactgaaagt aaaagagcta tcatattgga aacagtggaa gatgaatgag tttgatctga
     4381 gtctatagat aaattgagct tctcttctat ttccccagat ttctgtaaga cactggttcc
     4441 agcttcaaac atactatcaa taacttcacc aatagtattc ctgccctctg gtttatgatt
     4501 ggtgaaataa gccgtataag cctgaatata aaaagaggtg gctaatccaa aatgtaccag
     4561 ccatcttccc catttatatt ttcgtataaa acgagtatca tacagcacct tccccagatt
     4621 taaatttccc agagcataca cacctgcaag aataaagaat ataactttta aggtaaaaag
     4681 tgccattaaa gggaattgaa ctttaaagat ggtcccaaaa atccagtaag cagtaaaaaa
     4741 aggcagagtt actcgtaaac cttttaggac ctgtttataa agagaagaat ccagaagcat
     4801 aaagcttaaa aaaatcccta tcaataataa tattttttgc caggaagcac ttattgcagc
     4861 tatcagagct aaaactaata ttaataaacg aagcccaaga tgctgttcgc ttaaaactga
     4921 tagccttttt cccactaagg tttctcattc ggaatgaaaa atgggttttt cttaccctgc
     4981 tccccgggag attcaggttg aggaaagaaa ttattttctg gattcttact catattaata
     5041 gtttccgtct cctccttttt ctctggaacg gaaagaatgg attcttcttc tggaactata
     5101 gtggtatctt gagcttcaga ttccgaggat agctcttccc tcagactatc cgattcagtg
     5161 aaggaaagaa gcaactgatc aaatctatta agctggaaat tctttccatc aaagaatttt
     5221 gctgtaatcg tggtgtattc agtatctgaa tcatattcca gaacctttgc cagatatggt
     5281 tttgcagcag tggtgtctct tgcttcaaag gtataatacc atcctaaacg aaaattagcc
     5341 ttaagcttaa tctcggggta atcagagttc accagttcat tcaatataat cagcatagaa
     5401 tctggataag tttccgcata tccaagagct ttatccagta ataaatattg ctcttcttcc
     5461 ctggggtcaa ttaaacgaat aggtttcccc gcaagcatat tctccagagc tcgggtatat
     5521 ttattagcgg gatattcttc ttgcatccta ttataaagag cttgcatctg ttcattacca
     5581 ccagggattt tttggtaaac acaggcttga gaaaatagtg ccagcggaat aatctcgcgt
     5641 tcaaatctat ctaatatctc atctaaagca gctaacctgt cctgattatt cttcagttca
     5701 gcatgcagag tagtcaaaga ttgttccagt tttttccttt gttctgcagg attaactatc
     5761 tcttcttcaa tctttagact atcggaatcc gtagaaagat tcgtattatc tgctatggtt
     5821 aaactatctg aagctataag agaatcccct aaagcagttt ccgcttgtga aaaagataaa
     5881 tttaaactat cttcttcaga gatagcaggt agagtttcta tcaatacaga aattgaatct
     5941 atagcacttt gttggtagga aattacattt agcaaggaat cctttctggc agtaattata
     6001 tctctggtat caattagctt ttgatacatc aatatggcag aatctggaag agcaaattga
     6061 ttcaaataat attctgcagc agtcatataa taatctatca attcttgcag tttttgttcc
     6121 gggtcaagtt tatctttatt tttcaaaaaa tctatagcac tggctttctt ttgggcaata
     6181 gttgcaagtt cagagttagg gaattcggtt cgcactttgt tgtaagcagt catagctttt
     6241 gtcaaatcac cagcctggta aaaataatat tctcccagat agtaattagc ttccgcagaa
     6301 ctttgagtgc gaggatagga tttggtgata tctgccactt cggcaatacc ttgttccgta
     6361 tcaccctgag ctatcagtaa acgcgctttc aaaacccttg cttgagcaat tttgtcggga
     6421 cgattttcat cctttagtag cttcagtaaa gtcttccagc tggcttcata atcaccaaga
     6481 tagaattgat ttaaagctat ataataccga gcttccagtt taagcgattg agggatacga
     6541 gggtctctgg ctatcttttc aaattctcgt aatgatttag cgtaatcttt ttggataaag
     6601 tagtttttac caaaagtgaa gaaagcttca cgatattcct tagatttagg ataattagta
     6661 atcagctttt caagccagta ttgggctttg atatagttct tgtcttcaat ttcaaattct
     6721 gccagagtca agagagcacg aggatgtaat tttttctgac tggggtcaag gataaattct
     6781 tccagaattt tatttgcctg ttctttctgg tttatctccc gatacacttt tgccagatag
     6841 atatgtgctt ctccccagta aggactttcg ggaaagttat taatcaaagc ttcaaattgg
     6901 tcttttgctt gaaaagcact attcccttta tagtaaaggg cacaagccat tataaaaagg
     6961 gcatcatcag cttctttgcc aggattggga cgactaagaa tgataccaca tttcttaata
     7021 gctttagtat attcttcaac tgcctggtta gtgggtctac cattagcatt aagaggtctt
     7081 gattgggctg atttataata tttacgggca ttataaagtg tgttagtctc catacacccg
     7141 cttagtatca atagaatcat cagcaaagga atatatcttt tcatattatc agattatgct
     7201 ttattctact atttgtcaat ataaatttat cccctcggtc tgcaccaatt ccagaagggt
     7261 tgatttcttc aaaaaattca ctgcagcatt tatatctgga taggttatgc ggtcttcttg
     7321 aagagtagga acaatttgcc taaaacttga tttcactttt tccaatactg aagaagaggg
     7381 aatatttcgc tggtctaatg cttgagctgc taccattaat tctattgcca ggacggttct
     7441 cacattttcc actacctgaa ttaatttatt ggctgagact gaacccatgc tcacaaaatc
     7501 ttcctggtta gcagaagtgg gaatattatc tacacaagca ggatgagcca atactctgtt
     7561 ttccgaaacc agtgatgcag cagtcagttg cacaatcata taaccagaat caatgccagg
     7621 attttttgcc agaaaagggg ttaaacccct atttaacgct ggattaagca tctgttctat
     7681 ccttctttcc gagatagatg ccagctccga tattgccaga gctaaggtat ctaagcaaat
     7741 tgccagaggt tcaccatgaa agtttcctcc cgaaataacc ttttcctctt cagggaaaat
     7801 gagaggatta tccgttgctg aatttatttc cacttccagc actcctctta cataatctat
     7861 agcatcacgc actgctccat gaacttgagg tgtgcatctc aagctatagg gatcttgcac
     7921 attttcacaa tttatgtgcg attcccgtaa ggggctatta ttcatcaagt tactgatgtt
     7981 ggaagcactg gctatttgac ccttatgcgg acgtaattta tgaactaagc tatcaaaagc
     8041 ggaaggagtt cctttcattg catcaattgt gcaggcagct atgatatctg catacttaca
     8101 caatctttct gcttctaaga gatttaatac tccgagagca gtcataacct gagttccatt
     8161 attcaaggca agaccttcct tagcttgcag ttgtaccggt tttatccccg ctctttgcat
     8221 cgcttctgct ccagaaataa tttcaccttc aaactcagct tcaccttctc ctataagagt
     8281 tagtgccaga tgcgaaagtg gggaaaggtc tccgcttgca cctacagaac ctctcatagg
     8341 aattatggaa tgtacacctt tattcaacat atcaactaaa gtttgtaagg tttccagtct
     8401 tattccagaa tgtcctttag ctaatacatt tatacgcaga agcataattg cccgagtttg
     8461 ttccacggta taggcaggtc caacactggt agaatgactg cggattaaat taacttgcaa
     8521 ttcagcaatg ttatctctgg gaatagctat ggaacttaac ttcccaaaac cagtattcaa
     8581 tccgtaaaca agttcacctt tgtctattat tttttccaca taatctctgc acttattaac
     8641 ttgttgaata caggcttcag ttaatgcgat aggagtgcgt ttataagcaa cctcttctat
     8701 cttttccaag tttaaactgt ttccatcaat ataaattgta gacatcaatc ttccttctcc
     8761 tattatactg caacattgga ttttaacttt gctgcagact taaataatag agtgcaacat
     8821 atatagagaa gccttttttg tcaaaccgca agtagtaaga gtatctctgg aaagcagatg
     8881 actgaaaatg gtaagaaata ctttcgttgc tacaatccaa tatggcattt aaaacctgtt
     8941 tttaaatttc ctaaccatta tatccagata ttttgacttt atcccttttt aatgaccacc
     9001 cattataaat tagccaacct aatattctac catatgagtt ttgctggtta tttaaatgca
     9061 atgaatctct ttttttatct ttctaacatc catccataag ataatatact tattctttta
     9121 ttgaaattaa taatatgcta ataatcaaaa agttgtatct cctaaatgtc caagggaatc
     9181 tacaaaatca aataaataaa ttgacagaaa agcattggtt caaaagtatg acataataat
     9241 ttagaaactg tctaaaatgt atagcccaag tccctaattt tgcaggggct tatagttctt
     9301 taggaggaac catgatatcc tgtttgcagg atacttatca aagtatcaaa tggaataaag
     9361 tgcaaactat tattcctaca aactcaaaaa cagttttaac cagcgagatc ctgtgcggaa
     9421 acgaaatacc tgttaaggtg ttttctccct gtgggatttt tatggtctga aaacagggag
     9481 gaataattga gcaaaggtaa ttttgaaaat cgcattagaa tcaaactaaa agcttatgac
     9541 catcggttat tagatcagtc ggtggcagaa attgtgaaaa gcactcgtaa cacaggtgcc
     9601 aaagtcgttg gacctattcc tcttcctact gataggacca tatatactgt tttgcgttct
     9661 ccacatgtgg acaagaaatc ccaggaccag tttcaaatgt tggtgcataa gagattgatt
     9721 gatatattaa acccaactca acaaaccact aatgctttaa agaagcttag tttacctgct
     9781 ggtgtccatg tggaaattaa agccaacaca aggagctaac aatgcttgga ttaattggta
     9841 agaaaatagg tatgactcag atttttgacc cggatggaaa agttattccc gtaacggtta
     9901 ttcaagcagg accttgtaga gtggtctata aacgtagcaa ggctaaagaa ggttatgatg
     9961 ctctccagtt agggtatgaa gaaatagagg aaaaaagaag taatcgccct aaaattggac
    10021 attgtaagaa aaatggtagt cctgtttacc gttatctcag ggaattccgt ccctcaaaga
    10081 gtcaaaatat tgaagatata gaagtgggca cagaatttaa ggcggatatc tttcaaccca
    10141 atgaaaaggt tagtataagc tctcgttcca aaggtcgtgg ctttacagga gtaatgaaac
    10201 gtcatggctt tgctggtttt atgagctcac atggtgttca tgaatctttc cgtggtcctg
    10261 gctctatcgg tcaatgtgct cagccttctc gcgtgtttaa aggattaaag atggcaggac
    10321 atcacggaaa tcagaaagtg acagttcatc atctcaaggt cgtaaaagtt gatcctgaac
    10381 aaaacctgat aatggtaaaa ggtgccgtgc caggacatcg taattcattg gtcataatcc
    10441 ataaggaatc ataggaaaaa acaatggcaa cagcaataaa atttaactcc attggtgaaa
    10501 agattgatga gatagaactg ccagcccaga tttttgaggt agatgttaat tctcccaagg
    10561 ttctcttgaa tgaagttgtt acacagtatt tacttaacca acgtcaggga actgttcaaa
    10621 agaaaaatcg ggctatgaca gcaggaagca ctcggaaaat ctatcgtcaa aaaggcacag
    10681 gaagagccag acagggctct attagaaatc ctgttcgtgt tcatggtggt cgcgctttcg
    10741 ctattttacc aaaaaattgg tatcgtccta ttcccaaaaa aaagaagcgt cttgctttga
    10801 aggtcgcatt aaccgataga gcaagagatg gacgtatctt cattattgaa tcttttgatt
    10861 tttctgaacc gaatactaaa caggctcgtg aaattttgaa taaaatcgtc ccagataaag
    10921 gatggaaact ggtagtaaca gatggacacc atttccctac cgttaaatcc ttcaacaatc
    10981 taccagatgt gatgactgac cgagcagata gtctctatgc ttatgaaata ttgaagagtc
    11041 attatattgt tatgactcaa gatgctctca acaaagttga ggaggtattc agcaaatgat
    11101 acatccaaga aatataatta tctcccctat aatcacggaa aaaagtgaga aattggcacg
    11161 agacaataat atgtatacct ttaaggtaag cattaatgcc aataagatag agattaagaa
    11221 agctattgag aaaatcttct ctgttaaggt tctggatgtt aataccataa gaatgctcgg
    11281 aaagccaaaa agtctgggta aatacaacgg caaacgtccg gattggaaaa aggcagtagt
    11341 aacccttaga gagggtgata agattgccga ttttgaggtc taaggagaag acaatgggta
    11401 ttaaaaaata tagacctatc accccatctc ttcgttttcg taccggttat acctttgaag
    11461 agctgacgaa gaaaaaacct gaaaaatccc tgattaagcc agtacgtaaa accgcaggaa
    11521 gaaataatag tggaagaatc acctgtcgtc atcggggtgg tggacatcgt cgtcattacc
    11581 gtattattga ttttaaaaga gataaatttg gtattccagc taaagtagca gccatagaat
    11641 atgaccccaa tcgcactgca agaatagctt tactgcatta tatagatggt gaaaaaaggt
    11701 atatccttgc tccagaaggt ttacaagtgg gcaccaaagt tatgtctggt ccagaagcag
    11761 aaattgcgat tggtaatgct gttcctctgg aaagaatacc cttaggagct acagttcata
    11821 atatagagtt aaaaagaggt aagggtggcc aaattgctcg ttctgccgga acttttggtc
    11881 aagtggtagc taaagatggt gattatgttc atgtaaagat gccttccaac gatgtgcatc
    11941 taatccgtaa agagtgttta gctactattg ggcaggtaag taatatagaa cacaatctgg
    12001 tgcatatagg aaaagcagga agaaatcgct ggaaaggtat tcgtcccacc gtccgtggtg
    12061 ttgctatgaa tccaatagac catcctatgg gtggtggaga aggaaaatcc tctggaggaa
    12121 gacatcctgt ttctccttgg ggtaaactcg ctaagggtgg aaaaacccgt aaacctcata
    12181 aatattccga taagtatatc gtgaaagccg ttaaaaagaa ataagggaag gaaataatgg
    12241 cacgttcaat taaaaaagga ccttttgttg acgcccacct tctaaaaaag gtagaagcac
    12301 taaatgccga aaataaaaaa gcagttatta agacctggtc tcgtcgttct atgattatac
    12361 cagattttat aggtcatacc ttttccgttc ataatgggca taaatttgtt cccgtttata
    12421 taaccgagaa tatggtaggt cataaactgg gagagttttc gcccactcgt acctaccgtg
    12481 gacataaaga aaagaaaact aaggcaaaat aaaggagcaa attaaatgga agctactgca
    12541 aaactccgct ttgcccgtgg ttctgctcgt aaagcaagat tagtgttaga ccttattaga
    12601 tataaaccgg tccctgaagc ccaaaatatc ctgcgattct ctcgcagagc tgcagctgta
    12661 ccaattagta aattgttgga ttcagctatc gctaatgcta aacttcagga cccaaaactg
    12721 gacttaaata aaatggttgt ttccgttgcc acagccgatg agggtccaca aatgaaacgc
    12781 tatatgccca gagctatggg aagagcttat atgattcgta aacaaacctg ccatatagct
    12841 ctggaaatca gaacgttgga agattaggag gaatagcttg ggacaaaaaa ttcaccccat
    12901 tctttatcgc ctcggtgtta ataaagatac cgaatctata tggtttgccg atggtcagac
    12961 ctatgtggat tatctgcaag aagatatccg tatccgtaaa tatatccata aaagactgga
    13021 agataaaatg gtctctcgcg tgcaaatctg gcgaaaaacc aactctatta caattgatat
    13081 tcacactgct cgtccaggtt tagtgattgg caaaaaaggt gaagatatag aacgtcttcg
    13141 caatgaatta aatgtgctga ttaacaagaa ccgtaagagt cccatagctg tgtctattaa
    13201 tattgaacaa atagataagc tgtggctgga tgcaagatta gtgggcagag aagtggctcg
    13261 tcaacttgaa gagcgtgtac ctttccgtag agcaatgaaa tccgctattc gtaatgttat
    13321 gaaagaagga gcacaaggag taaaagtcca ggtttctggt cgtttaggcg gtgcagaaat
    13381 agctcgcaca gaaacctata aacagggaag aacacctctg cataccatta gagcagatat
    13441 tgattacgct tgtgtggaag ctcttaccac ctatggtgta attggaataa aagtttggat
    13501 atataaaggc gatatcctcg gtgaggagaa ataaatgtta gcaccaaaga aagttaaaca
    13561 tcgtaaggta atgaaaggca gaaggaaagg ccttgcttgg cgtggaaata ccgtagcttt
    13621 tggagattat ggacttatgg ctttggaaga tgccttcatt accagtcgtc aaattgaagc
    13681 tgcacgtatc gctataacac gatatatgaa acgagccggc aaagtatgga ttcgtatatt
    13741 cccggacaaa cctataacta aaaaaccagc tgaaactcgt atgggaaaag gaaaaggttc
    13801 tccagaatac tgggtggctg ttgtgcgtcc cggtagagtt atgtttgaga ttgaaggagt
    13861 aagtccagaa gaggcaaaag aagctatgcg gcttgcctct cataaattac ctataaagac
    13921 tcgtataatt gcacgagaag gaattgaatt atgaagatgg aagaaatgcg tgaaatgact
    13981 aatgaagagc ttcagaccag gctcgaagaa ttgagaatag aactttttaa cttacgtttt
    14041 cagaaatcta agaacctgct ggaccgccaa gatagaccaa gacttgtgag aagggatatt
    14101 gctcgtatcc tgactctctt gaaagaaaga gaaaagaatc agtgaggtga agatgcaaaa
    14161 tacgcataaa atgattaaac agggtgtagt ggttagtgat aagaacgata agactattgt
    14221 cgtcagcgtt caacgccaat ttattcatcc tttatataaa aaaacagtcc gcaggcataa
    14281 gaaatttatg gctcatgatg aacataacga agcgcatgaa ggagatattg ttcaaattat
    14341 agaacatcgt cctatgagtg ctcgtaaacg ctgggctctt tataaaattg tagagcgaag
    14401 caaataaagg agttttggaa tgattcaggt tcaaactatg ttgaatatcg ccgataactc
    14461 cggtgccaga aaagcaatgt gtattaaagt gcttggaggc accaggcgta aatatgccac
    14521 cgttggcgat gaaattgtag tagccgtcaa atctgctact ccaggtggta aagtaaaaaa
    14581 aggcagcgtg gaacatgcgg taatcgtgcg aactgctaaa gaaattagaa gaccagatgg
    14641 ttcttatatc cgcttttctg ataacgctgc agttattatt gatgaaagac acgaccctaa
    14701 aggaacacgt attttcggac ccgttgctcg tgaattgcgg gaacatcatt atatgaagat
    14761 agtttcttta gcaccagaag tgctatagga gattactatg gcaaataaaa ctgaaaaaaa
    14821 acagcctaaa ttgagattga aaaaagggga ttatgtagtt gtgatatctg gtgacgataa
    14881 agggaaaaaa ggacatattc tcaaagctta tcctaaaaca ggtaaggtta tcgtagaaaa
    14941 agtgcatatg attaaaaaac acaccaaacc ctcacaaaac aatcctcagg gcggtatcat
    15001 ctctatggaa gctccgattg acgcttccaa tgttatgctt tttaatgata gattaaacac
    15061 tgtttctaaa ccagtatata aggtgcgaga aggtcgtaga gtacggatat gtaagaaatc
    15121 tggtgacgag ttgtaggaga tgttactatg aatagattga aagaacatta taaaaatacg
    15181 gttatcccaa atctgcagaa gcagtttaac tataaaaatg tacatcaagt gccaaagctg
    15241 gataagattg tggtaagtat gggagtggga gctgctactc agaataaagc aattctggat
    15301 aatgccgtaa aagatatgga acagataacg ggaagaaaac cgatagtgac tcgtgccaaa
    15361 aaatccattt ccaattttaa actcaggaaa ggaatgccaa ttggatgcaa ggtcactttg
    15421 cgtgatgaaa ttatgtatga attctttgac cgtctggtct ctatagttat ccctcgtatc
    15481 cgagatttcc atggaattcc aaccgattct tttgacgggc gaggtaattt ctctatcgga
    15541 ttgaaagaac aaactgtctt tccagaaatt gaatatgata agattgatac tatacgaggt
    15601 ctaaatatca atataattac cactgctcgt accgatgagg aaggaagggc tctattgaaa
    15661 gaacttggta tgccctttca acataaagct taaggagata ttatggcaag aaaagcatta
    15721 ataatcaaac aacagcgtac acctaagttt aaagttagaa aatataaccg ctgtaagata
    15781 tgtggtcgtc ctcgtgctta tatgcgtgat tttggtatgt gtcgtctctg tttccgtaag
    15841 tatgcttcag agggacaaat accagggata accagagcca gctggtaata ttttaggagg
    15901 aaataaatga gcattactga tccgatagct gatgcgttga ccaaaattcg caatgcatat
    15961 cgtgccggac atcagcaggt gataatcaat cacagtaaat taacagaagc aatagttaag
    16021 attctcgccg aagaaaattt tgtgaatagc gttcaggttc tggataagga ccccgaacaa
    16081 aagataaatt ataagcgtat actgattaat cttcgctata ctaatgatgg aaaacctgtc
    16141 ttacaaggtc tggaacgagt atccaaacca ggaagaagag tttatgttcg tgccaataac
    16201 cttcctcgcg tatacaataa tacaggttgt gctattatat ctacttctca aggagttatg
    16261 gtagatagag atgcacgcat aaaacgcgtt ggtggcgaat atatctgtaa ggtgtggtag
    16321 gaggttgtta tgtcacgcat aggaagagcc cctattaaat ttgaccagga tactcacata
    16381 gaagtaaaag ataacattgt atctgtgaaa ggaaaactgg gtgaattgca atataaactt
    16441 atgcccggaa ttaccgtaga aatttcagat ggagttttac aggtaaaaag aagtaatgat
    16501 agcaaggacc aaaaagcttt gcatggctta actcgagcgc tattgcagaa tatggtaagg
    16561 ggcgttagtg aaggatatca aaagaatttg caagtgattg gaaccggcta ttcagcagaa
    16621 agaataggtc cctggctaaa attgagtcta ggctattctc atgatattat tatggaagtt
    16681 ccagaaggat taactgtgga aactgaagct gtgcctcgtt ccaagacctc acgaactgat
    16741 ttacaaagca taattagagt taaaggaata gataaacaat tggttggaga atttgctgct
    16801 gaaatccgtg cttgtcgtcc cccagaaaat tataaaggaa aaggtattcg gtatgaaaat
    16861 gaatctgtca ttatcaaagc tggtaaagct gggacgaagt agatgaggag tgaaaaatga
    16921 ttaaatccag taccaaaatc aaagtagaac atcgcaatcg ccgtagagct gctatccgca
    16981 agaaattgcg cggaacagca gagcgtccac gtctggttgt gttccgtagt ctaaaacata
    17041 tctatgctca gattgtagat gatactgcag gtaagactct ggttactgct tccacaagag
    17101 gtaaggatat tcagatacct gctggtttga agaagacgga acaaagcttt ctcgttggtc
    17161 aaaaactggg tgaaaaagcc ctcgctgctg gaattacgaa agtagctttt gaccgagcag
    17221 gatacaaata tcacggtcgg gttaaagcac tggcagatgg tgcccgaaaa gccggactgg
    17281 acttctaaaa gggaggaata gtgaattacg aatataatca agcagaagaa caaaaactgt
    17341 ttgaaaccgt tatagatact aaaagagtag caaaagtagt caaaggtgga agaaacttta
    17401 gtttcactgc tattgttgtt gtaggagacc gtgcaggtaa tgtgggagtg ggaaatggaa
    17461 aagccaatga aatttccgat gctattcgca aagccaagga aagagctcaa aaaaatatgt
    17521 ttaaagtccc cattataaaa gggactatcc cccacgaaat aatcgctcgt tttggagcaa
    17581 gtaaggtgat gatgaagcca gcttctgaag gaactggaat cattgctggg ggaactgctc
    17641 gtgctatctt tgaagcagct gggattcaaa acatactttg taaatctttg ggctcaaaca
    17701 ctccttgtaa tgtggtcaaa gctactgttc tcggtcttaa atctatgcga accctttctg
    17761 atgtagctaa gcttcgtggt aaaaccgtgg ctgagatcac cggtcaggag gcgaaatgaa
    17821 gattaaagtt actcaagtta gaagcactat taaccgacca gaaaagcaga aactggttat
    17881 taaatcctta ggtttaggac gtcctggtag aagtagaatc catgatgata atccctgcat
    17941 tagagggatg attaataagg tttctcacct ggtcaaagtt gaagaattaa aggaagaata
    18001 atatgcttac tctttccaat cttgaaaaac ctcaaggcaa aaaagccaaa aaacgtttgg
    18061 gtaaaggaca gggttcggga actggtcatc aagctggtcg tggtaataaa ggacataaag
    18121 cacgcaccgg tggaaaaatc cctgcctatt ttgaaggtgg tcaaatgcct ataaaccgtc
    18181 gtcttcctaa acgtgggttc aaaaatgtct ttcataagaa ttatcgcgtc cttaatcttt
    18241 ctaaacttca ggatatagaa gagactaatt ttgatattac caagctggaa atgttggggt
    18301 taattcccgt taaagggaaa aaaggtaaat accccgttaa ggttctggct tccactactg
    18361 aggaattcac caagaccgtt catattagag ctaatgcctt ctccaaaaag gctaaacaac
    18421 tcatagaagc gaacggcggt aaggcggagg taatctaact tgtttaagac tcttgcgaat
    18481 gtctttcgga ttcccgatct gcgaaagaaa atcttaatca cactcttaat cctaaccctt
    18541 tatagattgg gaagttttat ccccatccca ggggttagtg gaactgaatt aaaatctttc
    18601 tttgctcgac agaccggtag catttttgac ctgcttaatc tctttgtggg gggaaatttt
    18661 gaacgagctt ccatttttgc tctcggaatt atgccctaca tcacagcttc tatcgtgatt
    18721 cagctcttag gaagtattgt cccctatttt gaaaagctga gaaaagaagg agcagatgga
    18781 caaaagaaat tgaatcagat tactcgctat tgtaccgttg ctttagctgc tttcaatgct
    18841 atcacagtta ctgttggact ggttaatatg catggtgcaa ctggtccggt ggttcctcaa
    18901 ccaaactttc tattccatct aactggagtt ataactctgg ttacaggaac aatgattgtt
    18961 atgtggttgg gcgagcaaat cacggaatat ggtatcggaa atggaatctc cctaatcatc
    19021 tttgctggaa tcattgcccg ttatccagaa ggttttatca atctcttcag aaccagattt
    19081 aatagcgtat caggagtgat aacttcgctt ctggctatag ctgtaatgat tttagctaca
    19141 gcagccatca tattcgttac cgaagcagtg cgtaagattc cagttcagta tgccaaaaga
    19201 attgttggaa gaaaggttta tggtggtcaa agcacctata tccctttgcg ggttaataca
    19261 gcaggagtta ttccaatcat ctttgcacag tccattttaa tgtttcccgc tactcttatc
    19321 accctctttc aaggtggaga agcggaagtc ggttctttct ggtataatat gcgtatctgg
    19381 ttttcacctg gacactgggt ttatactatc atctatgttg cactaattat tttctttgcc
    19441 tatttctata cagctattgt gctcaatcct acagaaatgg cagaaaatat gattaaatat
    19501 ggcggacata ttcccggaaa aaaaccaggt aaaaaaactg cagaatatat ttcctctgtt
    19561 cttactcgta taactcttcc gggagcagtt ttctttgctt tgattgcctt attacctgaa
    19621 attatgacca atgtgttcaa gctacctttc tattttggag gaacaggttt aattattgtc
    19681 gttggagtcg ctctggatac attgaaacag atagaatcac atcttattat gcgtcactat
    19741 gatggcttta tgaaaaaggg aaagcttaaa ggtcgcacca gctaattatg ataatacgca
    19801 aatctcctgc cgaaattgat aaaatccgta tcagttgcca gattcttggt gaactgctgg
    19861 atttactggc agagaaaatt atgcctggcg tgagcacctg ggatcttgat gcctttgctt
    19921 atgatttcat cattaaaaat ggtgcaaaac ctgctttcaa aggttatcag gttcctggct
    19981 taagtccttt cccctcaact ttgtgtacat ctcttaattc tgttattgtg catggtttcc
    20041 cctctaagaa ggtaattctt aaagaaggag acattattag catagatgtg ggagcaatca
    20101 aggacggata ctatagtgat gcagcccgaa cctatgccgt aggaactatt tccccagatg
    20161 ctcaaagatt aatggaagtt acccttaatg cactttataa aggaatagaa gctgcgattc
    20221 caggaaatag agtaggagat atatcaaatg caattggtac ctatgttgcc tctatgggtt
    20281 attatgtggc agatgatttg accgggcatg gtgttggacg ctatcttcat gaagagcctc
    20341 agatcccaaa tgctggtagt gctggacatg gacctcgtct tcaaccaggt atgactttag
    20401 ccatagaacc tatggtcaat atcggcacca atagagtaaa agagaatggc tgggaatttt
    20461 ctgtggcaga tggaacttta tctgcacatt ttgaacatac tatcttaatc accgatagtc
    20521 aacctgagat attaaccgtt gctaaaggag agagagtatg agtaaaacag gtgttataga
    20581 agtggaaggt atcgtcaccg aggcacttcc taatacaacc tttaaagtgc agctggagaa
    20641 tgggcacact attctggctc atagctccgg caaaatgcgg atgcactata tccgtatcct
    20701 tccaggagat aaagttaaag tggaactttc tccctatgac ttaactcgtg gcaggataac
    20761 ttaccgctat aaataaaaat tgcaggaaac gactgcactc ttgcaaggag atataatgaa
    20821 agtaagatca tcagttaaaa aactctgtaa agattgtaga attattaaac gtaacggtgt
    20881 aatccgggtt atctgcagct ctaaccctaa acacaaacaa agacagggtt aaggaggagt
    20941 aatttggcac atattgcagg aatagaacta cccaaaaaca aacgtttatt cattggctta
    21001 acctatatct atggcatagg acgttcccgt gcgatagaaa tttgcaaaaa agccaatatt
    21061 gacgaaatga aaaaagtaga agatctaacc ccggaagagg aaaaactgat tcgcgatatt
    21121 gtccaaaatg aatatgtcgt ggaaggtgct ctacgcactc aggttgctat gaatattaag
    21181 cgcctgatgg aaattggatg ttatcgtggc ttaagacata aacggggact accagttcgt
    21241 ggacagcgta ctcataccaa tgccagaacc cgtaaaggtc ctcgtcctgg cggaattaga
    21301 aagaagaaat aggagagaac tgaactatgc ctaaaaaaac cagaccaaag aaaaaacgca
    21361 ctcgcctttc ctttgatgaa ggtctaatct acattcattc cagcttcaac aacacaatag
    21421 taaccttaac tgaccgaagt ggaaatgtgc ttgcctggtc aagtggagga aaaatcggct
    21481 ataaaggctc tcgtaaagca actccatttg ctgctcaaat ggctgcttct gaagttgcta
    21541 aagctgccca agatatgggt atttcccgcg ttggactcat cgtgaaagga cctggtagtg
    21601 gtagagaatc tgctctgcgc gctgtaaatg ccgctggaat gaaagtaaca atgataaagg
    21661 acgccacacc cattcctcat aatggatgtc gtccaccaaa agccagaagg atttaaaaga
    21721 ggaaattatg gcaaaatata ctggacctaa agccaaatta tgtcgtagat ttggtgaaaa
    21781 tatctatgga cctcaaaaat acgacaagat tctaagtaaa cgtaagttcc ctcctggtca
    21841 gcatggaagt agtatgcatc gtaaacccag tgattattct ttacatctaa atgaaaaaca
    21901 aaaacttaga tatacttatt gcctactgga aagacagttc agaagatact tccagattgc
    21961 agataaaatg caaggtatca cgggtgataa tttgttaaaa ttgctggaaa gaagattgga
    22021 caatactgtc tatcgtatgg gttttgctac caccaggatg caggctcgac aatttgttaa
    22081 tcatggacat tttatggtta atggaaaaaa agtagatatc ccttcctatc tacttaagcc
    22141 aggagatatt atagaagtcc gtcccaaaag caggggtata aaacctttag tggaagcctg
    22201 gaacaattca gaggcaagtt caccctaccc ctggttaact gttgataaag aaaatatgcg
    22261 cggtcaattt gttaatatcc ccaatataga ggatattcct actacagttg atatgcgttt
    22321 ggtggttgaa tactactcca aataatggct ttacaagcga ggagtttata ttatgttata
    22381 tcttgaacct ttacaaatgc ccgaaaatgt ggattacgaa aaacaaacct attctcccac
    22441 tttcggacac tttgaaattg gaccgctgga acctggtttt ggcacaacct taggaaattc
    22501 gcttcgtcgt atactcctat cttccattca gggctcagct gttcgttttg ttagaataga
    22561 aggtttacat cacgagttta ctccgattcc cggaactaat tcggattata tagaccttat
    22621 tttgcggttg aaacaattgg tcatctattg cacttctatc gatgaagtta cggttactct
    22681 tgaacataaa ggtaaaggaa ttattaccgc agctaaaatt caggaacacc caaatgtaaa
    22741 aattatcaat aaagacctct atttgctgga agtaactgaa gatgttgatt ttaaaatgga
    22801 tttaatcatt ggaaatggac ggggttatgt tcccgcagac cgccaaaata cagaaggtaa
    22861 acctctggga tttattccta ttgattccat ctattccccc gttctgaaag tgaatttctc
    22921 tgtggctcat cagcgagtta aagaaagaat gaactacgac aagctgctta tagatatcta
    22981 caccaatggt gctatagagc caagcattgc cctatttctt tctgctaaac tgctgcgtga
    23041 tatggcagga aagatttgct tatttgaaac tgaacctcaa tacatacgtg atgtctatat
    23101 tgacccggaa ttggaagaaa aggaacgtat cctatcttta cctgtcaaag acattgaact
    23161 ttccgtccgt gcggcaaatt gtctggaagc agcaaatatt aagaccatcg gagaactggt
    23221 ttctaaaact gaacaggaaa tgttaaaata tcgtaacttc ggcaaaaagt ccctggaaga
    23281 aatcgtccaa aaattaaaga aatatgacct ccatctcgga atggatgttg aaggaattta
    23341 tcgtcaaatt agggaagctc gcagtcgcgg tattaagaca gaagaaactg ctcccaacaa
    23401 agaagaacag gaagaaaaat ctgttactcc acctcgtaaa cctgttccta ctactaaaaa
    23461 gaaggtgaaa aatgcgacat agagttgaag gtagaaaatt tggtagagaa actgaccaac
    23521 gccgtcttat gatgaataat cttgtcaagt ctatgataga acatggacag attactacca
    23581 ccctggctaa agccaaagaa atgcgtagat atgttgaacg tgtagtcact tatggaaaaa
    23641 atgataccgt acattcccgt cgtcttgctt ataaagtgct gggagaccga actctggtaa
    23701 aaaaactatt cactgaaatt gctccggctt tccaggatag aaatggaggt tatacccgca
    23761 ttattaaagc tggttatcgc agaggagatt ccgctcctat ggctgttatc caatttgttg
    23821 aatcctctac aattactcct aaaaaagagc atattaaagc gaaagacctg aataaataag
    23881 caagcctctc ataaatatta aaatggacta cccgataagg tagtccattt tttattcttc
    23941 tgaaaaaaga acttatttaa cctactatcc tcagattcct aaaagtcccc tattttaaaa
    24001 tctccaattc cataaatatc aactggttac atccctcttt ttacttttct atcacttctt
    24061 gatttatctc caacttatgt ccaacttatg tccagcttat gtccagctca tgtccgaaat
    24121 tggacataag ctcaagatta actggggatg gtttgcactt tttttgttag gattaaggtc
    24181 ttcaaagaga ctaaatggat cttgaccaaa aggttacaga gaatttatcc tattataatg
    24241 agcaaaataa aaaagatgcc cttatggttc aagtttagag acaaagagaa cccaaactgc
    24301 aacactatta ctgtatgaat tgttgccatc acagtctatg tagcaaagaa agaagattct
    24361 ttattctaat tgaataggat taataataat gccaggttat tccctgcgcc ttatcactaa
    24421 attcttattc gctctattaa tgtaaattat cgctatctaa cccgctacct ggataacatt
    24481 tcctaagtaa taacaataat ccattggaaa accctaataa acaactaaat agaaaattaa
    24541 cgaatctaaa taacttcaaa tctttggaat cgttaaattc ttttactcaa atctggttcg
    24601 cggattactt taatagaaaa aagactaaaa aaaatgaata ctcttaagaa aatattttta
    24661 tttcccttga caaaaaatga aaagagtttt aaatataatt taataaaatt aggggataga
    24721 atgatgaaaa agataatttt atttttgatg gtgctgtcat ttatattgcc tttcagtgca
    24781 gtatacttta aaattgggga ttactataca cctggtaatg caaattatgt tgctatgtct
    24841 gacaatattg tttatgtagc agctaactaa gggggtttac agattataga tgtttccaat
    24901 cctcagaatc cagctcttgt gggctcttat gatacacctg gtgaagcact ctccgttacc
    24961 gtttctaata atattgccta tgtagcagat aaggaatctg gtttacagat tatagatgtt
    25021 tctaatccac agaatccatt tctgcttggc tcttatgata cacctggtta tgcattatct
    25081 gtttttgttt ctaataatat tgcctatgta acagatgact atgcgggttt actgattata
    25141 gatgtttcca atcctcaaaa tccagctttt ttgggctctt atgatacacc cgattgggca
    25201 gaatctgtta ctgtttcaaa taatattgcc tatgtggcag catgggatgc aggtttacag
    25261 attatagatg tttccaatcc tcagaaccca gctcttttgg gctcttataa tacacccggt
    25321 tctgcatact ctgtttttgt ttctattaat attgcctatg tggcagcatg ggatgcaggt
    25381 ttacagatta tagatgtttc caatcctcag aatccagtac ttattggatc ttatgatacg
    25441 cctagttatg cactttctat tactgtttca aataatattg cctatgtagc agattattgg
    25501 gctggtttac agattataga tgttactaat cctcaaaatc cagctctttt gggctcgtat
    25561 gatacacctg gtactgcatt atctgttttt gtttcaaata atattgccta tgtggcagat
    25621 gtgagtcctg gattacagat tatagatttt ctatacgcac atggttatta tcatcgattt
    25681 ccagatgcag cagcatggga tgcaggtttg cagattatag atgtttccaa tcctcaaaat
    25741 ccagctcttt taggctctta tgatacgcct agttatgcat actctgtttt tgtttctaat
    25801 aatattgcct atgtagcagc atgggatgca ggtttacaga ttattgatgt ttccaatcct
    25861 cagaatccat ttattttagg ctcttatcat acaccttatt atgcatactc tgttttcgtt
    25921 tcaaataata ttgcctatgt agcagatt
//
LOCUS       DAORZF010000023        25511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_1632_length_25511_cov_361.037712, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000023 DAORZF010000000
VERSION     DAORZF010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 25511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25511
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1632_length_25511_cov_361.037712"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..644
                     /locus_tag="PK015_04070"
     CDS             195..644
                     /locus_tag="PK015_04070"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MraZ"
                     /protein_id="HOL33681.1"
                     /translation="MSGPFIGYSENAVHNNRVIIPAAFKRKFAEEANRTVVITVGPLN
                     SIALYPLDSWFATLDALAKGDEIQRRFRTRLIDCAVTEAELEGPGRIRIPERQLAEAG
                     IKDSVIVKGEMHYITLWNPDDYYRLRSQNLEANLKEYDTNIYQITGL"
     gene            641..1570
                     /gene="rsmH"
                     /locus_tag="PK015_04075"
     CDS             641..1570
                     /gene="rsmH"
                     /locus_tag="PK015_04075"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOL33682.1"
                     /translation="MRDYHIPVMLKQCINFIAPKPGGIYVDATIGGGGHSLGLLESCP
                     DIHLIGFDQDSEALLEAHKTLFPYQDRVELIQANFSRMRTELAFRQIKTVSGILFDLG
                     VSSHQLETPERGFSFDLDSPLDMRMDQSSDLTAEIVLNELSQKALTRIFYDYGEELNA
                     SRIAGKIVKIREEQPFKTTGDLVKVIESVVGKGTKASLKTKARIFQALRIYVNQELEI
                     LEQALKDAINILEPGGRIVVLSYHSLEDRIVKNVFRSAAEGEEQTSEASFLNSTKKRK
                     LQLLTRKPLQADDEEINNNPHSRSVKLRAAEKI"
     gene            1580..1936
                     /locus_tag="PK015_04080"
     CDS             1580..1936
                     /locus_tag="PK015_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33683.1"
                     /translation="MRARLCIFLLLLLIITFINFWNAHQIVGQTRELAKLEKQVAAEK
                     NINTELKVERDDLRSGKNIASLVKVELDHLNPQEEQGKIIYVHEPANTEDKTSYCIID
                     LFTTKAQAREVKIQLD"
     gene            1940..4114
                     /locus_tag="PK015_04085"
     CDS             1940..4114
                     /locus_tag="PK015_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOL33684.1"
                     /translation="MHSRHKILVFVFGVLTLLWSSYLFILQIVDPFHFADERRIRYTP
                     YKEILIPTRGSIYDANGNLMVSSISFYQIDIDRKAVSLWAKEKNISLNEAYNILSEAI
                     GKNCSITPKQVLQRLTLNDKLTSVQITNKVREMELERILAEFEKNKLPGLTYSFASMK
                     RIYSQELLAARLLGSVRAVSDGYDVETQSRSIYQLSGICGLESTYNKYLAGEYGWREV
                     VFDAKHRRMPYPNLHEKPEKDGYNLHLTIDANIQAVVENALYEGLTKYGAKNAGAVIM
                     DPNTGRILAMAGVSPDDKSLDPAEVRVRANIPVSFLFEPGSTMKPLTMLAALDRHLVR
                     PNEYFECGVYYVQGRKISDTHMYGSLRPVDIISKSSNVGIAKIAERVGPKHLYEKLIS
                     LGYGQKTGLNLQGESSGMFAKLDNWDGYTLHSVSFGQGMAVTALQHATAFCAVANGGK
                     IMKPYLVDYITDDNGNIVERTEPEVLRQVCSKAAADTVRSYMQAVVDRGTATHIKADY
                     ITIAGKTGTAQKAAEGGRGYINGKYTSVFVGMVPADAPKMVMVIFYDEPSPGYHFGSM
                     SAAPTFKKILDDILFMPNCNIVAFDQRLIQSSKRMPDLRGKHIKQAEAILNQYGFSYK
                     IEGADSSSVVIDQFPKPNVSVDPAHPITLKIGRGKKEAETELLPGVMPDLCGLTIRKA
                     MQVAARENISLKIKGSGIVRRQSIQPGSRITNNTTCIIEASI"
     gene            4111..6960
                     /locus_tag="PK015_04090"
     CDS             4111..6960
                     /locus_tag="PK015_04090"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOL33685.1"
                     /translation="MKLKEILNLFQEHKILVNYQGLEENWEFKGKICTDSREVQAGDI
                     FVCIKGLHSDGHNFIDEVKKKNAAFIVGEIPIDEALPYIQVTESRKAAALLAKLYYHN
                     PSAEFRLIGITGTNGKTTTSMLLYRALMELGYCCGWIGTLGYYINDEHYSTHHTTPDI
                     LELNQILSRMVEKGVKYVSMEVSSHALALDRVYGLEFDYCLFTNLSREHLDFHQTMEE
                     YGSAKKKLFEPTIQHKAVGLINIDDNFGRKLFEELKSVNAYVYSIGTSDADFIIRTEH
                     NHLPYSTSGNRFSLQTPEGIINIRSSLLGSFNMNNLAMCAATLNLMGFEARQIEQCLN
                     IAKPVKGRFEPVPNDRGIKVFVDYAHTPDAMENVLQACQDLEHKRILCLFGAGGDRDK
                     GKRSLMLQIALHYANAVIITDDNPRFEDPDAIIRDIVAGTELWFPWWIIRNRKEAIHS
                     ILRLAQAGDIVLLCGKGHENYQEIEGVRYPFDDAEIASEFLHSEDAFQLRQDELTLPV
                     DKLMLELLAGIPLTVNGKYKPPESYHFLSTDSRTIVPNSIFFALTGSHFNGNDFLPQV
                     LSDETILGVGTIENQGWKNYIQVPDTLSLLASLCRKYLQLFDIYKIALTGSTGKSSTK
                     EMLAQVFNSSSPTLKTQANENNMIGLCKTIPRIRPEHRYGVFELGTNAFGEIAALADV
                     CNPDAGIIINIGPSHLEKLLDEDGVFREKTALFNRPLEIRLFDGDDPRFALYKDKGKS
                     VGFSDTSDFHITDLECDEASCHFKINGEPFTIPYSAPFLAKNASFAIAMGMLKDIPLP
                     QIKTAIANPVQLDLRLQVEKTDSYILIVDCYNANPISMQSAIEYWHKLEPEREHIAIL
                     GDMLELGTQAPMYHNMIGTILTEKGVDRLITVGNLSRYYHSSDNSKQIEHFDSVDELI
                     KSDILKTLTPGAVILIKGSHSVQLEKVLPILHKGS"
     gene            6963..8066
                     /gene="mraY"
                     /locus_tag="PK015_04095"
     CDS             6963..8066
                     /gene="mraY"
                     /locus_tag="PK015_04095"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOL33686.1"
                     /translation="MLYHLLYPLSKYSIVFNVFRYITFRSIGAFVTALLFVLIAGPPL
                     VKFLKGQSAVELIDEDKPKRHKAKEGTPTMGGLIILFGLLIASLLWNDLTNRPILMMY
                     LTAVWLGVLGFLDDYLKNFLKISKGLLPKYKIWGQIGVGLFLTLAIYFSTNVVDNVTQ
                     IQIPFLKNTYLQLGWLFIPIMVIYITGISNAVNLTDGLDGLAAGVMIFSALGLGIMSY
                     LKGNYVIANYLKLEFIPTAGELTVFIAGLIGALIGFLWFNAYPAEVFMGDTGSLTLGG
                     ILAVLSILLKEQIFFLIIGFLFIAETLSVIIQRTHFRYTKKKYGTGKRVFLCAPIHHH
                     FEMKGMHESKIVIRFWIVATLLLAIGLSTIKLR"
     gene            8077..9432
                     /gene="murD"
                     /locus_tag="PK015_04100"
     CDS             8077..9432
                     /gene="murD"
                     /locus_tag="PK015_04100"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOL33687.1"
                     /translation="MFNPKTSYAILGLARSGIAVAYKIKELGGTAYLSDIKTEEQIEY
                     AEKLKQDFECEFGGHTNKLFQFEQWIVSPGIPLNLPIIQKAKQLGIPLICDIEFGYQI
                     KSPDSKIIAVTGSNGKSTTVSLIQHILQHMGYNSILAGNIGNALTSYPIEKPGIDFIV
                     LELSSFQLDLIDTFKPDVAVLLNITPDHLDRYDSFQTYIDSKFRIFINQNQEDFAVIC
                     LDSEPIAERQDRIKAQLLRYSLEKTPPDCEAWLNQDAIQIGVKHKLSVRDLKIRGPHN
                     YANTMAALLAVNALTHNLDAAMEAAKTFPPLPHRLELVAKVNDIAFYNDSKATNTDAV
                     KSALNAFDQPVRVIMGGSDKGEDFSVLTPLLKKAAKKVYLTGATADKMYEIWKDDLPL
                     EIIDDFSQCIHKAFYDSVPGDIILLSPACASFDKFKNYEERGEVFKQIVNSIAREYEK
                     E"
     gene            9419..10555
                     /locus_tag="PK015_04105"
     CDS             9419..10555
                     /locus_tag="PK015_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOL33688.1"
                     /translation="MKKNKSIQYIVSYDKVILICYLSLCLIGILMMLDITSMQSMSMF
                     YHHLIYFIFSIAVLGIIFYFLDLEKLRPLNILWIFLSIALLILVLIYGSTVKGGKRSI
                     RFGPLSIQPSFLARLALIFFYAGFLAKKQAIINTTNFPELLKELAPLLVYTAIIFVLI
                     FMEKHLSTLIVGSATLLGMLYYAGLRKRFVLAIVIVGLIAGIFIINKGDEFRSARIET
                     YKVYSLFNPHRPEPGNSPDEYQVRESLTALTSGQIIGTGVARGRAKHNFLPEARTDYV
                     YSIIGEEFGFLGAIIVLGLHALLFFRILKIASEQDNLYYKYLCVGIALNIFLNVLLNT
                     GVAMSLLPPTGNTLPFISYGGTALLIDSMSIGVVLNISAKRRQL"
     gene            10616..11719
                     /gene="murG"
                     /locus_tag="PK015_04110"
     CDS             10616..11719
                     /gene="murG"
                     /locus_tag="PK015_04110"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOL33689.1"
                     /translation="MNNLSSNQNLRIRILFGCGGTGGHIFPAIALAQEFKKLGNEVMF
                     IGNRGGMEEHIVQEEGFPFRAIKVQKLYRKFNPANLLFPFRLASSWLESKKIMKDFKP
                     DAVICTGGFVSGPVGLASIIMKIPLYLHESNSYPGLTTKFLAPHSRITFTVYDKTKSF
                     LKKSRVQKVGVPLAEKKEKATLSSLTEIGLNPEKTVILVTGGSQGSQAINSVIEKTLP
                     QIIKRDWQLIWQTGKQGYSNYANKFSNLSGVYIFDFSPLLSSFYRFATLAITRAGAIT
                     IAELEANAVPAILIPLPTAAENHQFYNAREQEKKGVAILLPQAELNTQTLLNAMDKIL
                     NNYDFYKNNLESLPPNTAKQDIIQAILKDLGKE"
     gene            11724..13142
                     /gene="murC"
                     /locus_tag="PK015_04115"
     CDS             11724..13142
                     /gene="murC"
                     /locus_tag="PK015_04115"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOL33690.1"
                     /translation="MLGKTRKIHFTGIGGIGMSGIAEFLHNQGLEITGSDLKKTDITR
                     HLESLGIKITEGHDPALVKDVDVVVRSSAIKDDNPEIQAAKALKIPVIRRAEMLAEIT
                     RMSFSIGISGTHGKTTTTSMTGLVLEAAGLDPTIIVGGKIKNYGSNNVMGSGQYIVVE
                     ADEYDHSFLTLSPCIAGITNIDIDHLDCYQNLDEIKKAFIEYANKVPFFGSVIACLDD
                     PGVQSILPEINKRIVTYGFSRQADIQAQNIKMKNFMAEYDVSYKSYRLGRINLKVTGK
                     HNILNSLMAVGIGLELDIPFKFIKEGLESYSGVYRRFELKGTANGITIYDDYAHHPTE
                     ITATLDGFKEGTKRRIVTLFQPHLYSRTRDFYKEFANAFFSCDCLILAPIYPAREEPI
                     EGVSSKLIADAAIQSGHHNVVLIEKNEDIVPLTLSFLKPNDILITMGAGNIWQYGEEI
                     LKELKKAVDKNSKSENDKNLEK"
     gene            13182..13961
                     /locus_tag="PK015_04120"
     CDS             13182..13961
                     /locus_tag="PK015_04120"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOL33691.1"
                     /translation="MKYKELQKEVPNRKRRGHSRYFIYFILMLIGLAAFVYGAYYLFS
                     TVSWFNIEQIQISGNSTVSDSLLIQATQSFKGKNLFSISKDEVISKVKKFSRIKDVKV
                     QRRLFHTLNIIVTERKSCLYVKTLEGDLFPIDDEGVVLEKFGSVYNENLPIVNLLINN
                     AEIHPGKPIKNASLAKILEVHHQIKTESPEFLSNISEYYTINNTVYIIDARNGLRLIP
                     STKNLARQLSRYEFVKDNGNVSNNAILDLRFDNQVVVKSGN"
     gene            13963..15240
                     /gene="ftsA"
                     /locus_tag="PK015_04125"
     CDS             13963..15240
                     /gene="ftsA"
                     /locus_tag="PK015_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOL33692.1"
                     /translation="MKQNIITALDIGSSNIRCIIAKIKTPEHIEVLGISQNPSEGIEK
                     GIVKDIQAVAEAVKKTIKEAENAASTKAINIFTNVTGEHIRTVLSDGRISIPTNSPNE
                     PGEITQEHIDQVIADARNSVKIQKGFERSQILHAIPQCFIIDGQDDIRNPIRMTGFHL
                     MTKVLTIFGEVTPLRNLAKCIELAGYDLDPDNFILNHIAISESVLSDDERRLGTLLID
                     IGGGTCDLSLFHRGVLEKVLVVPMAGADITEDLAIGLKTTLSNAEYIKTHYGVALANS
                     VDPDMEIEIEGISGRASQRKSQVLVSHVIQHRVEEMLTLCYEHLKNFYTPELVTAGIV
                     LTGGTAKLQEIDAVVKNIFNLNVKIATPNLNRLNGSISRLEDSDFATCVGLLYYAASL
                     DRDYKNAVRIPGNFQASKLFDKIKNIIKEFTSS"
     gene            15252..16442
                     /gene="ftsZ"
                     /locus_tag="PK015_04130"
     CDS             15252..16442
                     /gene="ftsZ"
                     /locus_tag="PK015_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOL33693.1"
                     /translation="MIEFDEKPGQIGTNIKIIGVGGAGGNAVNTMITKELTGVEFIVA
                     NTDNSDLMKNKAKMKLQMGKKLCGGLGTGANPELGERAAEEAKDEIKSHLEGADMVII
                     ASGMGGGTGTGASPVIAKLAKDLDILTLAIVTTPFPFEGSKRMTNAKEGINKLVEYVD
                     TLIVIPNSRVSEIYADRPLLDAFAMADNVLYEAAKAISDIININGLVNVDFADVKTVM
                     QNGGFALMGSGVGEGENRAILAAEAAINNPLLSDISLQGCQSLLINVSGGKDFKMSEL
                     EEVTSVIVNVTGSDANIIMGVIIDPDMEEKVTVTIIATGLQNITHQIGDFISLSQGMV
                     KPEKHTKTANPKYPTEDEIEDIFHVLNINQTAHKEEDTENKKKEPEKLNPRTDLPSFL
                     KALD"
     assembly_gap    16844..16943
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            17418..18707
                     /locus_tag="PK015_04135"
     CDS             17418..18707
                     /locus_tag="PK015_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HOL33694.1"
                     /translation="MQKEGLNDKDIIALVDCNNFYVSCERVFNPSLEGKPVAVLSNND
                     GCIVSRSNELKALKIPMGAPGFKYEGLIQKNGGVLLSSNYALYGDMSSRVMEVLNQFS
                     PEVEIYSIDEAFLKLNDLPISNLEEYGRQIKHIVKKYTGIPVSVGISRSKTLAKIANH
                     YAKHFAGYQGSLALMEADKIEKALKQTPVEEIWGIGRQYAKFLRQNKIETAWDLRNAP
                     EDFILHYLTKVGYKTVLELQGYSCIDLEEAPSPKKSIVCSKSFSKQVSDLSELEEAIS
                     TYITRGAEKLRHQHSVAGFMMVFLNTNRFKEGPQYNNSTQTKLSVPTAYTPDLIKIGL
                     ELLRDVYLPGFEYKKAGVMFADLIDEKDVPLNFLEPNYLDDKRKKLIDAIDKLNRVWG
                     RDTVFFASSGIKKDWEMKRAKLSPFYTTRWQDLPKVK"
     gene            18709..19314
                     /locus_tag="PK015_04140"
     CDS             18709..19314
                     /locus_tag="PK015_04140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOL33695.1"
                     /translation="MSYYSIAEPVNSRQKIKHSEFLAFLFPIDSVEEAESIISQHRKE
                     YPDATHHCFAYISGFNRETQFYSDSGEPTGTAGKPILNALLSANMTNILAIVTRYFGG
                     IKLGTAGLAKAYSGSVQAALLKANKIPAIPYSTYHLELDYQGINPLKYKISSFNGHII
                     EENWDNRAELKVQIPEEKSKEWEEFINGLKPKFYLNYKKEE"
     gene            19320..20141
                     /gene="amrB"
                     /locus_tag="PK015_04145"
     CDS             19320..20141
                     /gene="amrB"
                     /locus_tag="PK015_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOL33696.1"
                     /translation="MIRRTSHAGSFYPRFKEQIIAMINQWIAGEKVAPDSEKALSLLV
                     PHAGYIYSGACAAKGFHYISQQPFDSFVILHPSHQGIHFDYSVAPFEQYETPLGMLNQ
                     DEELFDLLTRDNPDIANQIRYHEMEHSMEVQLPFIKYFFPEAQICPVMIGRPYPQVAM
                     ELAQRLKSAINGTEKRVGIIVSSDLSHYYPATRAERMDSVFAEQFLTLDPEKLWHSIV
                     SESCEACGIGGILTLLYYVKEIPEAKAKVIHYTHSGKVTGDNTQVVGYLSALVYR"
     gene            20151..20696
                     /gene="amrA"
                     /locus_tag="PK015_04150"
     CDS             20151..20696
                     /gene="amrA"
                     /locus_tag="PK015_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOL33697.1"
                     /translation="MVISNEAKKALLKLARDSIAYRFTGIPPVKPTYPELQEKRGVFV
                     TLNKHGQLRGCIGYIQGYKSIVDSVIEMAQAAAFEDPRFPPLRQSELPELEIEISILG
                     DLIPVNNIDEIVIGRDGLYIQHPYGSGIFLPQVPVEWNWDLPTYLKELCRKAGLNDKD
                     WMDPRAQLFRFEAEVFSESEF"
     gene            20747..20822
                     /locus_tag="PK015_04155"
     tRNA            20747..20822
                     /locus_tag="PK015_04155"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20780..20782,aa:Gly,seq:gcc)
     gene            20833..20906
                     /locus_tag="PK015_04160"
     tRNA            20833..20906
                     /locus_tag="PK015_04160"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20865..20867,aa:Cys,seq:gca)
     gene            20917..21003
                     /locus_tag="PK015_04165"
     tRNA            20917..21003
                     /locus_tag="PK015_04165"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20951..20953,aa:Leu,seq:taa)
     gene            21005..21080
                     /locus_tag="PK015_04170"
     tRNA            21005..21080
                     /locus_tag="PK015_04170"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21038..21040,aa:Gly,seq:gcc)
     gene            complement(21086..22840)
                     /locus_tag="PK015_04175"
     CDS             complement(21086..22840)
                     /locus_tag="PK015_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOL33698.1"
                     /translation="MDKHIKWIVNQWKREKWFVLLMLVFTLASSAVSIAYPILFGRLL
                     DLLKNILGEPARYPNPMQEINRIIWVFVALGFAQFITGFYPAIRGWMNIRFENFLRKY
                     YFKFVLTKDFRFFHKFRTGDIVTRLTDDLTDYSKISWFLCSGIFRAVNSFSLIVFALV
                     VMFSINVRLTLLSIASLPIMMVVFYFASDKLYRNFELNQKAISNINSQLEMSFSGIRI
                     VKAFVSEEKYNRFFDKALAQRFKTEMGVVKLNALLQLIYGYIDYIAQIGVILFGGYMA
                     VKGTISVGTFYMFYSYLGMMIYPLLDLPQLFVSGKQAFVNIDRLEEIKDFPSFYDNWR
                     GDIKPKDIEDITFQEVRCTYPEKDEPAIRNCNLEFKKGEKLLILGSTGAGKSTIANLI
                     VGLLLPEKGEILINEIPFPKIELNALRELIGYVPQEPVLFTGTIKENILMPVDEISEE
                     EYQIAVKTAQLEQELASFPDKDETLVGQRGLGVSGGQKQRITIARALIKRPQLLVLDD
                     ITASLDAENEEKLWEDLNANYPGISAIVISHRLSTLHYVDKVLFIDEQGCTHQGTHEE
                     LVFKNQEYHDFLHEHLKE"
     gene            complement(22833..24596)
                     /locus_tag="PK015_04180"
     CDS             complement(22833..24596)
                     /locus_tag="PK015_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOL33699.1"
                     /translation="MPNQKQNLKYYIHLLYPFIKKDIGLLGFGLFAMLCTSALQLVYP
                     LILANIVDKSIPSGNVSQMYRYGAMFIAAVLVSGALTYLQTILLSKLGVKVITKFKAQ
                     VFRHLLTLPVEWFNKQAVGELIARVESDAERVKALFSDLSIRIIGNLLFFIGIFLVMF
                     LKEWHVAAYILPGIIVGVIAYYYLIKYLSKFYRQIREKNALVTAKITDYVQGVPIIQS
                     LNLQNKVYNDLRNASDDKYKLETKTTYLEYGAQSFFMFLFEVFFIVMIIRITAPQILA
                     GIVTLGTLIVFIDYTYRMIWPLMSISENVMQIQRSFVSLKRILELNELQSEDEIFTGK
                     RLPTFEHEIRFENVWFAYNNEDWVLKDISFTIPKGKKIALVGPSGSGKTTTVSLLCYF
                     YQAQKGQILVDGVPLSEIDFRAWRRKIGLILQDVFLFPGSILENVRVYNDAISEEKVK
                     EAINVVQLDEFIQEQRLGLDTELAERGQNISQGEKQLISFARALAFDAEIIVMDEATA
                     SVDPQTEARIQHSMERVFANKTVVVVAHRLTTILDADEILFFDKGRIIHRGTHQQLLN
                     ESSEYRKLVTLQMIGLEDTLG"
     gene            complement(24627..24704)
                     /locus_tag="PK015_04185"
     tRNA            complement(24627..24704)
                     /locus_tag="PK015_04185"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24667..24669),aa:Pro,seq:cgg)
     gene            complement(24867..>25511)
                     /locus_tag="PK015_04190"
     CDS             complement(24867..>25511)
                     /locus_tag="PK015_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33700.1"
                     /translation="QATVYNYSGNHRTLTINQRLDNYGTICNSPNTHWIFLTIAGDLY
                     NYSSIFNYQITMNGTQDQHFQHMADSSINVNYFTLVSDVGAAFWYYNNSLTPYNSSPT
                     NSININPTQYGTWQPRNPETGESGRHIIISTGEAHIDTPTAFTIYTDNGIPKLRWNKV
                     SNALFYHLFVSTTPEGLSTVTPITIYDTDLSDNIVTVDLEGVEPIQFYRVTAGY"
BASE COUNT         8210 a         4595 c         4813 g         7793 t
ORIGIN      
        1 aaaatgagat attttttcaa aaaactacat tttttacttg acaggaaaac ttgaatgtca
       61 tataagcatc acaggaagtc aaaaaatgtc aaaaaatgtc aaaatgcttt tctgaactga
      121 gttcacaaag acaaaaatag cgtgttaata cttttaaaaa aagaaagata aggatatata
      181 agaaggtatg aaagatgtca ggaccattta ttggttattc agaaaacgcc gttcataaca
      241 atcgggtcat cattcccgct gctttcaagc gtaaattcgc tgaagaagca aatagaacag
      301 tagttataac ggttggacct ctcaattcaa ttgccttata tccgcttgat tcctggtttg
      361 ccaccctgga tgctctggcg aaaggtgatg aaatacagcg tcgttttcgt accagattaa
      421 tagattgtgc agtaactgaa gcagaacttg aaggaccagg tagaatccga attcctgaaa
      481 gacaattagc agaagcagga attaaggata gcgtgattgt gaaaggtgag atgcattata
      541 tcacactctg gaatccggat gattattatc gtttaagaag ccagaatctg gaagctaatt
      601 taaaagaata tgataccaat atatatcaaa taacaggatt atgagagact atcacattcc
      661 agtaatgcta aaacagtgca taaattttat tgcaccaaaa cctggtggaa tctatgttga
      721 tgccactatc ggaggaggcg gtcatagttt aggtttactt gaaagctgtc ctgacattca
      781 tttaatcgga tttgaccagg attcagaagc tttgctggaa gcgcacaaga ccttatttcc
      841 ttatcaagac agggttgaat taattcaggc aaatttctct cgtatgagga ccgaacttgc
      901 tttccgtcag attaaaaccg tcagtggtat tctttttgac ttaggagtgt cctctcatca
      961 actggaaaca ccggaacgag gttttagctt tgaccttgac tctcctttgg atatgcggat
     1021 ggaccagagc tcagatttaa ctgctgaaat tgttttaaat gagttaagtc agaaagcatt
     1081 aaccagaatt ttttatgatt atggagaaga gttaaatgcc tctcgtatag cgggaaagat
     1141 tgtaaaaatt agagaagaac agccctttaa aactacaggt gacctggtta aagtgataga
     1201 atcagtagta ggtaagggaa ctaaagcatc gctgaagaca aaagcacgta tatttcaagc
     1261 attacgtatc tatgttaatc aggagctgga aattttagaa caagctttaa aagatgccat
     1321 taatatcctt gaacctggag gcagaattgt ggttttgagc tatcattctt tagaggatag
     1381 gattgttaaa aatgtttttc gttctgcagc agaaggtgaa gaacagacct cagaggcatc
     1441 attcttaaat agcactaaaa agagaaagct tcaattatta accagaaaac ctcttcaagc
     1501 agatgatgaa gagataaaca ataatccaca ttctcgcagt gtaaaattgc gggcagcaga
     1561 aaaaatttag ggggaaaaaa tgcgagccag actttgtatc ttcttgcttt tattgctaat
     1621 tataactttt atcaattttt ggaatgccca ccagattgtt ggtcaaactc gtgaactggc
     1681 aaaattagaa aaacaagttg ctgcggaaaa gaatattaat actgaactaa aagtagaacg
     1741 tgatgacctt cgtagcggta aaaatattgc cagcctggtc aaagttgagc ttgatcattt
     1801 aaatccacag gaagaacagg gcaaaataat ttatgttcat gaacctgcaa acaccgaaga
     1861 taagacaagt tattgcataa ttgatttgtt tacaaccaaa gctcaggcac gagaagttaa
     1921 gattcagctt gattaaaaca tgcattccag gcataaaatt ctggtttttg tttttggggt
     1981 tttgacccta ttgtggtcaa gttatctttt tattcttcaa attgtagacc catttcattt
     2041 cgctgatgaa agacgcattc gttatacccc ttataaagaa attttaattc ccactcgtgg
     2101 ttctatctat gatgccaatg gcaatttaat ggtcagttcc ataagttttt accaaattga
     2161 tattgaccgt aaagcagttt cactctgggc aaaagaaaag aatatctctc taaatgaagc
     2221 ttataatatc ctcagtgagg ctattggtaa gaattgcagt ataaccccta aacaggtttt
     2281 acaaagatta acccttaatg acaaactgac ctctgtccag attacgaata aagtccggga
     2341 aatggaactg gaaagaattc tggcggagtt tgaaaaaaat aaattacctg gactaaccta
     2401 cagttttgct tctatgaaac gcatctattc tcaagaattg cttgccgcaa gattgttagg
     2461 ttcggttaga gcagtatcag atggatatga tgtggaaact cagagtcgtt ccatttatca
     2521 acttagtggt atttgtggtt tagaatccac ttataataaa tatttagcag gagaatatgg
     2581 ctggagagaa gtggtctttg atgctaaaca tcgtcggatg ccctatccca accttcatga
     2641 aaagccagaa aaggatggtt ataatttaca tttgactatt gatgccaaca ttcaagcagt
     2701 agtagaaaat gcgctatatg aaggcttaac taaatatggg gcaaaaaatg caggagcggt
     2761 gattatggac cctaatacag gcagaattct ggcaatggca ggagtttctc ctgatgataa
     2821 gagccttgat cctgcagaag ttagagtcag agccaacatt cccgtcagtt ttctttttga
     2881 acccggctcc actatgaaac ctttaactat gcttgctgct ctggatagac atctggttag
     2941 acccaatgaa tactttgaat gtggagtcta ttatgttcag gggagaaaaa tcagtgatac
     3001 tcatatgtat gggagtttaa gaccagtaga tataataagt aaatccagta atgtaggcat
     3061 tgctaaaatt gcagaaagag tagggcccaa acatctttat gagaaattga tttcactcgg
     3121 ttatggtcaa aagacaggat tgaatcttca aggtgaaagt agcggtatgt ttgccaaact
     3181 ggataactgg gatggatata cccttcattc tgtttccttt ggacaaggaa tggcagtaac
     3241 agctcttcaa catgcgactg ctttctgtgc agttgccaat ggtggaaaaa taatgaaacc
     3301 ttatctggtg gattatataa ctgatgataa tggcaatata gttgaacgaa ctgaaccaga
     3361 agttttaaga caggtttgta gtaaagcagc tgcagataca gtccgttctt atatgcaagc
     3421 tgttgtagat agaggcactg ccacccatat aaaagctgat tatatcacta tcgcaggaaa
     3481 aacaggaact gctcaaaaag cggcagaagg tggaagagga tatataaatg ggaaatatac
     3541 ttctgtgttt gtaggaatgg ttcctgctga tgctcccaag atggtaatgg taatttttta
     3601 tgacgaaccc agtccaggtt atcattttgg aagtatgagc gctgctccaa ccttcaaaaa
     3661 gattttagat gatatactat ttatgcctaa ttgcaatatc gtggcatttg accagagatt
     3721 gatacaaagt tcaaaaagga tgcctgactt aagaggaaaa cacatcaaac aagctgaagc
     3781 gatattaaat caatatggtt tttcctataa gattgaagga gcagattctt catctgtggt
     3841 tatagaccaa ttccccaagc ccaatgtatc agttgatccg gctcatccta tcaccttaaa
     3901 gattggtcgt ggtaaaaaag aagctgagac tgaacttctt ccaggagtta tgcccgattt
     3961 atgtggtcta accatccgaa aagctatgca agtggcagcc cgagaaaata tctccttaaa
     4021 aattaagggt tcaggtatag tccgtcgtca atccatccag cccggttccc gtataaccaa
     4081 taataccacc tgtataattg aggcttctat atgaaattaa aagaaatctt gaatttgttc
     4141 caggaacata aaatactcgt taactatcaa gggctggaag aaaactggga atttaaaggt
     4201 aaaatatgca ctgatagtcg tgaagtccaa gcaggtgata tctttgtctg tataaaagga
     4261 ttacattctg atggacacaa ctttattgat gaggtcaaaa agaaaaatgc ggcttttata
     4321 gttggtgaaa taccaataga tgaagcctta ccttatattc aagtaacaga atcccgtaaa
     4381 gctgcagctt tgcttgctaa actctattat cataatccca gtgcagaatt tcgtttaatt
     4441 ggaataacgg gcacgaatgg caaaaccacc acctctatgt tattatacag agctttaatg
     4501 gaattgggat attgctgtgg gtggattggc actttaggtt attacatcaa tgatgaacat
     4561 tacagcaccc atcataccac accagatatt cttgaattga atcagatctt atccaggatg
     4621 gtagaaaagg gagttaagta tgtaagtatg gaagtatcct ctcatgctct tgctttggat
     4681 agagtttatg gattagaatt tgattattgc ctttttacca atctaagccg tgaacacttg
     4741 gatttccatc aaaccatgga agaatatggc tcagcaaaaa agaagctatt tgaacctacc
     4801 atccagcata aagctgtggg tctcatcaat atagatgata actttggcag gaaacttttt
     4861 gaggaactaa aaagtgttaa tgcttatgta tatagcattg gaacttcaga tgctgatttt
     4921 attatccgaa ctgagcataa tcatcttccc tattctacaa gtggcaatcg tttctccctc
     4981 caaactccag aaggaataat caatattcgt tcctcacttt taggtagttt caatatgaat
     5041 aatctggcta tgtgtgctgc gacactcaat ttaatgggat ttgaagcacg gcagatagaa
     5101 cagtgtttaa atattgctaa gccagtaaaa ggaagatttg aaccagtgcc aaatgataga
     5161 ggaatcaaag tctttgttga ttatgctcat actccagacg caatggaaaa tgttcttcaa
     5221 gcctgccagg accttgaaca taaaagaatt ctttgcctat ttggagctgg tggtgataga
     5281 gataaaggaa aacgctctct gatgcttcag attgcattgc attatgcaaa tgcagttatt
     5341 atcacagatg ataatcctcg ctttgaggac cctgatgcaa tcattagaga catcgtagct
     5401 ggaacggaac tctggtttcc ctggtggatt attagaaatc ggaaagaagc cattcatagt
     5461 atcttaagac tggctcaagc cggtgatata gttctgctat gtggtaaagg acatgaaaat
     5521 tatcaagaaa tagaaggtgt tcgctatccc tttgacgatg ccgaaattgc ctctgaattt
     5581 ctccattctg aagatgcttt tcaactaaga caagatgaac taacccttcc tgtagataaa
     5641 ttgatgctgg aacttttagc tggaatccct ctaacagtga atggaaaata taaaccacct
     5701 gaaagttatc actttctctc taccgatagc agaacaatcg ttcctaactc tatcttcttt
     5761 gctttaactg gctctcattt taatggtaat gacttcctac ctcaagtttt gagtgatgaa
     5821 actatactgg gagtaggcac catagaaaat caaggctgga aaaactatat tcaagttcca
     5881 gataccctat ctttacttgc ttcgctctgc cgtaaatatc ttcaactgtt tgatatatat
     5941 aaaattgccc taaccggtag cacaggaaaa agtagcacca aagaaatgtt agctcaggtc
     6001 ttcaactctt cttcacctac tttaaagact caagctaatg aaaacaatat gattggactt
     6061 tgcaaaacta tcccacgcat taggccagaa catcgttatg gtgtttttga actgggtaca
     6121 aatgcctttg gtgaaattgc tgccttagca gatgtttgta atcctgatgc aggtattatc
     6181 atcaatatag gaccctctca tctggaaaag ttattggatg aggatggtgt tttcagagaa
     6241 aaaacggctc tattcaatcg tcctctggaa ataagacttt ttgatggtga cgaccctcgt
     6301 tttgctctat ataaagataa aggaaagagt gtgggattca gcgatacttc agatttccat
     6361 ataactgatt tagaatgtga tgaagcttcc tgccatttca aaattaatgg tgaacctttt
     6421 actatccctt attctgctcc ctttctggct aaaaatgcat ctttcgctat agctatgggt
     6481 atgcttaaag acattcctct ccctcagatt aaaacagcca ttgctaatcc agtgcaatta
     6541 gacctccgtc ttcaagttga aaaaactgat agttacatcc tcatagttga ttgttacaat
     6601 gccaatccca tctctatgca aagtgctatt gaatactggc ataagcttga accagaaagg
     6661 gaacatattg ctattctggg ggatatgctt gaactcggaa ctcaagcacc tatgtatcac
     6721 aatatgattg ggactatact gacggaaaag ggagtagata gattaattac agtaggaaat
     6781 ctttccagat attatcattc ttctgataat tctaagcaaa ttgagcattt tgatagtgtt
     6841 gatgagctga taaaaagtga tattttaaaa accttgacgc caggtgcggt aattcttata
     6901 aaaggttctc attccgttca gttagaaaaa gtcttaccga ttttacacaa aggaagctaa
     6961 ttatgctgta tcatcttctc tatcctctgt caaagtatag cattgtattt aatgttttcc
     7021 gatatatcac tttcaggtct atcggagctt ttgttacagc tctacttttt gttcttatag
     7081 ctggaccccc tctcgttaag tttttgaaag gacaatctgc agtagaattg atagatgaag
     7141 ataaaccaaa acgtcataaa gctaaagaag gaacacccac aatgggtggc ttgataattc
     7201 tattcggact tcttattgct tctttgctct ggaatgacct gactaatcgt ccaattttaa
     7261 tgatgtatct caccgctgta tggttaggtg ttctcggttt cttagatgat taccttaaga
     7321 atttcctgaa aatatccaaa ggtcttttac ctaaatataa aatctgggga cagataggag
     7381 tgggtctgtt tcttacctta gctatctatt ttagcacaaa tgtagtggat aatgtaacgc
     7441 aaatccagat tccttttcta aaaaacacct atctccagct gggatggttg tttattccta
     7501 taatggtaat atacataacg ggaatatcta atgcggtaaa tttaaccgat ggattagatg
     7561 gactcgcagc tggtgtgatg attttttccg ccttaggtct gggtattatg agttatttga
     7621 agggaaatta tgtaattgct aattacttaa aactggaatt tataccaact gccggagaac
     7681 taaccgtctt tattgctgga ttaataggtg ctttaatagg ttttctctgg tttaatgcct
     7741 atcccgcaga agtttttatg ggagatacag gttctttaac tctgggggga attctggcag
     7801 tattatctat attgttaaaa gagcagatat tctttctgat aattggtttt ctctttattg
     7861 ctgaaacact atcagtgata atacaaagaa ctcatttccg atataccaaa aaaaaatatg
     7921 gaacgggaaa aagagtgttt ctttgtgcac ccattcatca tcactttgaa atgaaaggaa
     7981 tgcacgagtc aaagattgtt attcgcttct ggattgttgc cactttattg ctggctattg
     8041 gtttaagcac gattaaactg cgataaggag aataaattgt tcaatcccaa aacctcttat
     8101 gcaattcttg gactggcacg aagtggtatt gctgtagctt ataaaatcaa agaattgggt
     8161 ggcactgcct atctaagcga tattaaaaca gaagaacaga tagaatatgc tgaaaaatta
     8221 aagcaggatt ttgaatgtga gtttggtggt cataccaata aacttttcca gtttgaacag
     8281 tggatagtga gtccaggaat tccgcttaat ttgcctatca tccagaaagc taaacaactt
     8341 ggaattccct tgatttgtga tatagaattt ggctatcaga ttaaatcgcc agatagcaaa
     8401 atcattgcag taactggttc taatggcaag agcacaactg tctcacttat ccagcacata
     8461 ttacaacata tgggttataa tagtatctta gctggtaata ttggtaatgc tttaacttcc
     8521 tatccaatag aaaaacctgg aatagatttc atcgtgctgg agctaagtag ttttcagctg
     8581 gatttaatag atacttttaa acctgatgta gcagtcttat taaatatcac cccagaccat
     8641 ctggatagat atgattcttt tcagacctat attgattcca aattccgtat ttttataaat
     8701 caaaatcagg aagatttcgc ggttatttgt ctggattccg aacctatagc agaaagacag
     8761 gaccggatta aagctcaatt attacgctat tctttagaaa aaacacctcc tgattgtgaa
     8821 gcttggctaa atcaggatgc catccagatt ggagtgaaac ataaactttc agtacgagat
     8881 ttgaaaattc gtggacctca taactatgct aatactatgg cagctctttt agccgtcaat
     8941 gctttaactc ataatcttga tgcagcaatg gaagcagcca aaacttttcc tcctttgcct
     9001 catcgtctgg aattagttgc taaagttaac gatatagcct tttataacga ttccaaggct
     9061 actaatacgg atgcagttaa atctgcctta aatgcttttg accaaccagt tagagtgata
     9121 atgggtggtt cggataaagg tgaagatttc tctgtgctta ctcctctttt aaagaaagcg
     9181 gcaaaaaagg tttatctgac tggagctaca gcagacaaga tgtatgaaat ttggaaagat
     9241 gatttgcctc tggaaataat tgatgacttt agccaatgca ttcataaggc attttatgat
     9301 tctgttccag gagatataat tttactttct cctgcctgtg caagctttga taagttcaaa
     9361 aattatgaag aacgcggaga agtttttaag caaatagtca attctatagc ccgggaatat
     9421 gaaaaagaat aagagtattc aatatattgt ctcctacgat aaagtgattc ttatctgtta
     9481 tctatcactt tgtttgattg ggattttgat gatgctggat atcacttcta tgcaatctat
     9541 gagtatgttc tatcatcatc ttatttattt tatattctca attgctgttc tgggaattat
     9601 tttctatttc ctggacttgg aaaaacttcg tcctctaaat attctatgga ttttcctttc
     9661 tatcgcttta ttaatactgg ttttaatata tggttccacc gtaaaagggg gaaaaaggtc
     9721 tatcagattt ggtcccttaa gcatccagcc aagtttttta gcaagattag ctctaatctt
     9781 cttttatgca gggtttctgg ctaaaaagca agctataatt aacactacaa atttcccgga
     9841 actattaaag gaacttgctc ccttgcttgt ttatacagcg ataatttttg ttcttatttt
     9901 tatggaaaaa catctcagta ccttaattgt tggatctgcc actcttttag gaatgctcta
     9961 ttatgctgga ttgcgtaaac gatttgtgct ggctattgtc attgtcggct taattgctgg
    10021 aatcttcatt ataaataagg gagatgagtt ccgttctgca cgaattgaaa cttataaagt
    10081 ctattctcta ttcaatccac atcgtcctga accaggaaat tctcctgatg aatatcaagt
    10141 acgagaaagt ttaaccgcac taaccagtgg acaaattatt ggcacaggtg tggcacgtgg
    10201 tagagccaag cacaactttc ttcctgaagc cagaactgac tatgtatact ctattatagg
    10261 ggaagaattc ggatttcttg gtgcaattat cgttttaggt ttgcatgcac ttctcttttt
    10321 ccgtatttta aagatagcca gtgaacaaga caatctttac tataagtacc tctgtgtggg
    10381 tattgctttg aatatatttt taaatgtttt attaaataca ggtgtggcaa tgtctttatt
    10441 gcctcctaca ggaaatactc ttccctttat aagttacggt ggaacagcac tactgattga
    10501 ttctatgtct ataggtgtgg tgcttaatat tagtgctaaa aggagacaac tgtgaagtcc
    10561 tactggatat cttatatttc acagattgtc tctataatct cacttaattt tggaaatgaa
    10621 taatctatct tcaaatcaaa atcttaggat tcgcatcctt tttggctgtg gaggcactgg
    10681 aggtcatatc tttcctgcta ttgccctagc tcaagaattt aagaaacttg gtaatgaagt
    10741 tatgttcata ggaaatagag gcgggatgga agagcatatt gttcaggagg aaggtttccc
    10801 ctttagagcc attaaagtgc aaaagttata tcggaagttt aatcctgcta atctcttatt
    10861 tccttttcgt ttggcttctt cttggctgga aagcaagaaa ataatgaagg attttaagcc
    10921 tgatgctgtc atctgtactg gaggttttgt ttctggacca gtaggtctgg catcaattat
    10981 tatgaagatt ccactttatc ttcatgaaag taatagttat cctggactta ccactaaatt
    11041 tcttgcacca catagtcgta taactttcac agtttatgat aaaactaaat cttttctaaa
    11101 gaaaagccga gtccaaaaag taggtgtccc tcttgccgaa aaaaaggaaa aggctacttt
    11161 atcaagttta actgagattg gacttaatcc tgaaaagaca gttattttag ttacaggtgg
    11221 aagtcagggt tctcaagcta ttaattcggt aattgaaaaa acactacctc aaattataaa
    11281 aagagattgg caattaatct ggcaaacagg taagcaagga tattctaatt atgctaataa
    11341 attttctaac ctttcagggg tttatatctt tgatttttca cctttgcttt cttcttttta
    11401 tcgttttgcc actttagcta taacacgtgc tggagctata actatagcag aattggaagc
    11461 aaatgcagtt cccgcaattt tgattcctct tccaactgcg gcagaaaacc atcaatttta
    11521 taatgctcgg gaacaggaaa agaaaggagt ggctattcta ttacctcaag ctgaattaaa
    11581 cacacaaacc cttttgaatg ctatggataa aatcctgaat aattatgact tttataaaaa
    11641 taatctggaa agtcttccac ccaatacagc taaacaggat attattcagg cgattcttaa
    11701 agatttagga aaggagtgaa aaaatgttag gaaaaaccag aaaaatacat tttactggaa
    11761 tcggtggcat tggaatgagt ggaatcgctg aattcctgca taatcaaggt ctggaaataa
    11821 ctggttctga cttaaaaaag accgatatca ccagacatct ggaatctctg ggaattaaaa
    11881 taactgaagg tcacgatcct gctctggtaa aagatgtgga tgtggtagtc agatcttctg
    11941 ctataaaaga tgataatcca gaaattcaag cagcaaaagc attaaaaatc cccgttatac
    12001 gaagagctga aatgttggct gaaattacca gaatgagttt ctctattggt atttctggaa
    12061 ctcatggaaa gactaccacc acctctatga ctggattagt gctggaagct gcaggtctgg
    12121 atcctaccat catagtcggt ggaaaaatca aaaactatgg ttctaataat gtgatgggtt
    12181 cgggacaata tattgtggtt gaagctgatg aatatgacca ttctttcctt acactttcac
    12241 cttgtattgc tggaattacc aatattgata tagaccatct ggattgctat cagaatcttg
    12301 atgagataaa gaaagctttc attgagtatg ccaataaagt tccttttttt ggtagcgtaa
    12361 tcgcctgtct ggatgatcct ggagtgcaat ctatcctgcc tgaaatcaat aaaagaatcg
    12421 tcacctatgg tttttcaaga caggcagata tccaagcaca aaacatcaaa atgaaaaatt
    12481 ttatggcaga atatgatgtc agttataaaa gttatcgtct gggcagaatt aatttaaaag
    12541 tcaccggaaa acataacatt ttaaattctc ttatggctgt ggggattggc ttggagctgg
    12601 atattccttt caaatttatc aaagaaggtc tggaaagtta cagtggtgta tacagaagat
    12661 ttgaactgaa agggacggct aacggaatta ctatctatga tgattacgca catcatccta
    12721 ctgaaataac tgcaactctg gatggattta aagaaggcac caagcgtagg attgtaacac
    12781 ttttccaacc tcatctatat tctcgcacca gagatttcta taaagagttt gctaatgcct
    12841 ttttttcctg tgattgtctc attcttgcac caatttatcc tgcaagggaa gaacccattg
    12901 aaggagtgag ttccaaactg attgcggatg cggctatcca atcaggtcat cataatgtcg
    12961 ttctcataga aaaaaatgaa gatattgtgc ccttaacttt atcgttttta aagcctaatg
    13021 atattttaat tactatgggt gcaggaaata tctggcaata tggtgaagaa attttaaagg
    13081 aattaaagaa agctgttgac aaaaattcta agtctgaaaa tgataaaaac ttagagaagt
    13141 agattaaaaa ttaaaaaaaa ccgttttata gacctaatat tatgaaatat aaagaattgc
    13201 aaaaagaagt ccctaatcgg aaaagacgcg gtcatagccg ttacttcatc tattttatcc
    13261 tgatgttgat tggcttagct gcttttgtct atggtgcata ttatctcttt tctacggtta
    13321 gctggtttaa tattgaacaa atccagattt ctggcaattc tactgtttca gatagtttat
    13381 tgatacaagc gacgcagtct tttaagggaa agaatctgtt ttctatcagt aaagatgagg
    13441 ttatcagtaa agttaagaag ttttcacgaa tcaaggatgt aaaagtgcaa cgacgacttt
    13501 ttcacactct caatataatt gtaaccgaac ggaaaagctg tctttatgtt aaaactctgg
    13561 aaggtgacct cttccctatt gatgatgagg gtgtagttct ggaaaaattt ggctcagttt
    13621 ataatgaaaa tttacccata gtcaatcttc taataaataa tgctgaaatt catcctggta
    13681 aacctataaa aaatgcttca ttagctaaga ttcttgaagt tcatcatcaa attaagacag
    13741 aatcaccgga attcctttcc aatatttctg aatattacac tattaataac acagtctata
    13801 taatagatgc gagaaatgga ttgcggctta ttccaagcac caaaaatctt gcccgtcaat
    13861 tatctcgtta tgagttcgtt aaggataatg gtaatgttag caataatgcc attttagacc
    13921 ttcgttttga caatcaagta gtcgttaaat cgggaaactg agatgaaaca aaatattatt
    13981 actgcactgg atattggctc ttctaacatt cgttgcataa ttgccaaaat aaagactcca
    14041 gaacatatag aagtcctggg tatcagtcaa aacccttccg aagggataga aaaaggcatc
    14101 gtaaaagaca ttcaagcagt ggcagaagca gtcaaaaaaa ccatcaaaga agcagaaaat
    14161 gccgcttcca ccaaagctat taatatattt actaatgtta ctggtgagca cattcgcacc
    14221 gtcttaagtg atggacgcat ttctataccc accaacagtc ctaatgaacc aggtgaaatt
    14281 actcaagaac atatagacca ggtcattgca gatgcacgta atagtgttaa aattcagaaa
    14341 gggtttgaaa ggtctcaaat cctacatgct atccctcaat gctttataat tgatggacaa
    14401 gatgacattc gcaatcccat caggatgact gggttccatt taatgaccaa agtacttacc
    14461 atttttggtg aagttactcc tctgcgaaat ctcgctaaat gcatagaact tgctggttat
    14521 gaccttgatc ctgataattt tatcctcaat catatagcca tttcagaatc tgtgttatct
    14581 gatgatgaac gccgtctggg aactttactt attgatattg gtggtggaac ctgtgaccta
    14641 agtcttttcc atcgtggcgt tctggaaaaa gtgctggtcg ttcctatggc tggtgcagac
    14701 ataactgagg acctggctat aggattgaag acaacgcttt ctaatgctga atatatcaaa
    14761 actcattatg gtgtagcgct tgccaatagt gttgacccag atatggaaat tgagatagaa
    14821 ggaatcagtg gaagagcatc tcaaagaaaa tctcaggtgc tggttagtca tgtaattcag
    14881 catcgggtgg aagaaatgtt gaccctctgc tatgaacatt taaaaaactt ttatacacca
    14941 gaactcgtta ctgcaggaat agttctcact ggtggtactg caaagttgca ggaaattgat
    15001 gcagtggtga agaatatatt caatcttaat gtgaaaattg ccactcccaa tctaaataga
    15061 ttaaatgggt ctatcagcag actggaagat tctgattttg ccacctgcgt cggattgctt
    15121 tattatgctg catctttgga ccgtgattat aaaaatgctg ttcgtattcc cggtaatttt
    15181 caagcttcaa aactatttga taaaataaaa aatataatta aggaatttac aagctcttaa
    15241 agggggaacc aatgattgaa tttgatgaaa aaccaggtca aatcggcact aatatcaaga
    15301 taataggtgt tggtggtgca ggtggaaacg cagtcaacac gatgattacc aaagagttaa
    15361 ccggtgtgga atttatcgtt gccaataccg ataatagcga tttaatgaaa aataaggcta
    15421 agatgaagct acagatgggt aaaaaactct gtggtggttt aggaacaggc gccaatcctg
    15481 agttgggaga acgagccgcg gaagaagcta aagatgagat taagagccat ttggaaggcg
    15541 ctgatatggt cattatcgct tcaggaatgg gtggaggaac tggaactggt gcgtctccag
    15601 tcattgcaaa actggctaaa gacctggaca tacttacact ggcaattgta accactcctt
    15661 ttccatttga aggttcaaaa agaatgacca atgccaagga agggattaat aaacttgtgg
    15721 aatatgtgga tactcttatc gttattccta attccagagt tagcgaaatc tatgcagata
    15781 gacctcttct ggatgcattt gctatggcag ataatgttct ttatgaagca gctaaagcaa
    15841 tttccgacat tatcaatatt aatgggttgg ttaatgtaga ctttgccgat gttaagactg
    15901 ttatgcaaaa tggtggtttt gcccttatgg gttcaggtgt tggagaagga gaaaaccgcg
    15961 ctattcttgc agctgaagca gctattaaca atcctttact tagtgatatt agccttcaag
    16021 gatgtcaatc tctgttaatt aatgtctccg gagggaaaga ctttaaaatg agtgaactgg
    16081 aagaagttac aagtgtaatc gttaatgtaa ccggttcaga tgctaatatt attatggggg
    16141 ttattattga tccggatatg gaagaaaaag ttaccgtcac tatcatcgct acaggtttgc
    16201 aaaatattac tcaccaaatc ggtgatttta taagtttatc gcagggaatg gttaaaccag
    16261 aaaaacatac aaaaaccgct aatcccaaat atcctactga agatgaaatt gaagatattt
    16321 tccatgtttt gaacattaac cagactgctc ataaggaaga agatacggaa aataaaaaga
    16381 aagaacctga aaaacttaac ccgcgcaccg atttaccttc tttcttgaaa gctttagatt
    16441 aattatctta tcctctactt actaataaag tgggtacctt gcccacataa accaccggca
    16501 atccccctac cggtggtctc tttcttttta ttaaatactt tttaccttat ctatttgata
    16561 gaaatagaac ccttatccct ataaaatgaa aattgatata gctgaagtgt ccttccgaat
    16621 tccagatttt gtggttaatt ggataaatat ataaagtcag atgaaagaat ttatccaaat
    16681 caagtccaga aaccttgtgt ttccttaatc tctattcaat aataaataat tatcaaccat
    16741 cttagatatt gatatatttt atagcaccac ttataaaaag aaaatgtatc ctatctctta
    16801 aatctaattc tcccctggct ctctcttagc tctctcctgt tacnnnnnnn nnnnnnnnnn
    16861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16921 nnnnnnnnnn nnnnnnnnnn nnntcctatc tcttaaatct aattctcccc tggctctctc
    16981 ttagctctct cctgttacaa ataacaagta agagatagga aattaacaag atagcaattt
    17041 gatataaaaa atgtgtcaaa tatttgatag atatattaaa gctaatggta tggtatttag
    17101 gaagttataa attatttaac catttaaatc ttgctgataa ttgtaaatcc cactataatg
    17161 aaagaattgt gggaatttgt tctacctctt tctcgcttta taattcttat tttcggtcta
    17221 tctgttgccc agaccagcag attataactt aaaaataacg gatattttag atgaaaacac
    17281 caggatattt ttaaattcaa attcaattca gttaaaggat tgaaaatctt ctgtatttta
    17341 tcactttaac ccttgacctt tataaagcat attgacagca taagcaattg cactaaattg
    17401 taaaaaggag gagaaaaatg caaaaagaag gcttaaatga taaagatatc attgcgctgg
    17461 tggattgcaa taatttttat gtatcctgtg aacgggtatt taacccttca ctggaaggaa
    17521 aaccagtagc agttttatct aataatgatg gttgcattgt atctcgctcc aacgaactga
    17581 aggctctaaa gattccaatg ggcgcaccag gatttaaata tgagggatta atccagaaaa
    17641 atggtggtgt gcttctttct tccaattacg cactttatgg agatatgtct tcccgggtaa
    17701 tggaagtatt aaatcaattt tctcctgagg ttgaaatcta ctctattgat gaagcttttt
    17761 taaaattgaa tgaccttcct atctctaatc tggaagaata cggaagacaa ataaagcaca
    17821 ttgtcaaaaa atacactggt atccccgttt cagtcggaat ttctcgtagt aaaactcttg
    17881 ccaagattgc taatcattat gccaaacatt ttgcaggata ccagggttct ttagctttaa
    17941 tggaagcaga taagattgaa aaagctctaa aacagacacc agtagaggaa atttggggta
    18001 ttggcagaca atatgcaaaa tttttgcgcc agaataaaat tgaaactgcc tgggatttac
    18061 gcaatgctcc tgaagatttt attttgcact atctcactaa agtaggatac aaaacggttc
    18121 tggaattaca aggatattcc tgtattgacc tggaagaagc accctcccca aaaaagagca
    18181 tcgtctgctc taaatctttc agtaaacagg tatccgactt atctgaattg gaagaagcaa
    18241 tctccactta cataacccgt ggagctgaaa aactacgcca tcagcatagt gtagctggtt
    18301 ttatgatggt tttcctcaat accaatagat ttaaggaagg accgcaatat aataactcaa
    18361 ctcagactaa actttccgtt cccactgctt acactccgga tttgattaag attggtctgg
    18421 aactcttgcg ggatgtatat cttcctggtt ttgaatataa aaaagcagga gtgatgtttg
    18481 ctgaccttat agatgaaaaa gatgtaccgc ttaacttttt ggaacctaac tatctggacg
    18541 ataaaagaaa aaaattgatt gatgctatag ataaactgaa tagagtttgg ggtagagata
    18601 cggtcttttt tgccagttct ggtataaaaa aagactggga aatgaaacgg gcaaaacttt
    18661 caccatttta caccactcgc tggcaagacc tccctaaagt aaaataatat gagctattat
    18721 agcattgctg aaccggttaa ttccagacaa aagataaaac attcagagtt tttagcattc
    18781 ctcttcccta ttgattctgt tgaagaggca gaatccatta tcagtcagca tagaaaagaa
    18841 tatcccgatg ccactcatca ttgctttgcc tatatttctg gcttcaaccg agaaacccaa
    18901 ttctattctg actcaggtga accaactggt acggcaggaa aaccaatatt aaatgctcta
    18961 ctttccgcta atatgaccaa tatactggca attgtaaccc gctactttgg tggtataaaa
    19021 ctgggaacag ctggtttagc taaagcctat tcaggttctg ttcaggctgc ccttttaaaa
    19081 gcaaataaaa ttcctgctat cccctattct acctatcatc tggagctgga ttatcaaggt
    19141 atcaatcctt taaaatataa aatttcctcc tttaatggtc atattattga agagaactgg
    19201 gataatagag cggaactaaa ggttcaaatt cccgaggaaa aatctaaaga atgggaagaa
    19261 tttattaatg gtttaaaacc taaattttat ttaaattata agaaggagga ataagaatta
    19321 tgattagacg aacgagtcat gcgggaagct tttatccaag gtttaaagaa caaattatag
    19381 caatgataaa tcagtggatt gcaggagaga aagttgcacc tgattctgaa aaagctctat
    19441 cattactggt acctcatgca ggctatattt attctggagc ttgtgcagct aaaggtttcc
    19501 actacatttc tcaacagccc tttgatagct ttgtcattct ccatcccagt catcaaggta
    19561 tacattttga ctattctgtt gccccttttg agcaatatga aactccttta ggaatgctta
    19621 atcaagatga agaacttttt gatttactca caagagataa tcctgatatt gcaaatcaga
    19681 ttagatatca tgaaatggaa cactcaatgg aagtccaatt accgtttatt aaatacttct
    19741 ttcccgaagc tcagatttgt cctgttatga ttggaagacc ctatcctcag gtagcaatgg
    19801 aactggcaca aagattgaaa tccgctataa atggaaccga gaaaagagtt ggaatcattg
    19861 tctccagtga cctttcccat tactatcctg ctactcgtgc agaaagaatg gattctgtct
    19921 ttgcagaaca gtttctgacc cttgacccgg aaaaactatg gcatagcatt gtttctgaaa
    19981 gctgtgaagc ctgtggaatt ggcggaattc tcaccctact ttattatgta aaagaaattc
    20041 ctgaagccaa agcaaaagtc attcattata cccattcagg aaaagtgaca ggtgataata
    20101 ctcaggtagt tggatattta agtgccttgg tttacagata aggaggtaaa atggttataa
    20161 gtaatgaagc taaaaaagca ttattgaaat tagccagaga ttccatagcc tatcgtttca
    20221 ctggaatacc tccggttaaa ccgacttatc ctgaattgca ggaaaaaaga ggtgtctttg
    20281 taacacttaa taagcacgga caattaagag gatgtatagg ttatattcag ggctataaaa
    20341 gcatagtaga tagtgtgata gagatggcac aagcagctgc atttgaagac cctcgtttcc
    20401 ctcctttgag acaaagcgaa ttaccagaac tggaaataga aatttctatc ctgggagacc
    20461 tcataccggt taataatata gatgaaattg tcattggaag agatggttta tatatccagc
    20521 atccttatgg ttcaggaata tttttgcctc aggttcctgt agaatggaac tgggatttac
    20581 ccacctattt aaaagaactc tgccggaaag ctggtcttaa tgataaagac tggatggatc
    20641 ctcgggctca gcttttcaga tttgaagcag aagtattcag tgaatcagag ttttgagctt
    20701 gacaaaaaat ctgaggactt attttcagtc taaaaaatat atctttgcgg gaatagctca
    20761 gttggtagag cgcaaccttg ccaaggttga tgtcgcgggt tcgagtcccg tttcccgctc
    20821 cagatgaatt caggcgacat agccaagcgg taaggcagag gtctgcaaaa cctttatccc
    20881 cggttcgaat ccgggtgtcg cctccaaaaa tagcgtgccg gagtggtgga ataggtagac
    20941 acaagggact taaaatccct cggacttttg tccgtgccgg ttcaagtccg gcctctggta
    21001 ccatgcggga atagctcagt tggtagagcg caaccttgcc aaggttgatg tcgcgggttc
    21061 gagtcccgtt tcccgctcca ctcttttatt cctttaaatg ctcatgtaag aaatcgtgat
    21121 attcctgatt tttaaagaca agttcctcat gtgtaccctg atgtgtgcaa ccctgttcgt
    21181 ctataaagag caccttatcc acataatgca aagtagaaag ccgatgggaa atcactatag
    21241 cagaaattcc tggataattt gcatttaagt cttcccaaag tttttcttcg ttctcagcat
    21301 ccagtgaagc agttatatca tccaacacca gaagttgtgg tctttttatc aatgctctcg
    21361 caatagttat gcgttgcttt tgcccacctg atacacccaa tcctcgctgt ccaaccagag
    21421 tttcatcctt atctgggaaa gaagctaatt cctgctctaa ttgggctgtt ttcacggcaa
    21481 tctgatattc ctcttctgaa atctcatcca caggcataag gatattttct tttattgttc
    21541 cagtgaaaag cactggttct tgtgggacat aaccaatgag ttcacgcaat gcatttaact
    21601 ctatcttggg aaaaggaatc tcgttaatta gaatttcacc tttctcgggt aaaagtaaac
    21661 ccactataag attagcaata gtactcttcc ctgctcctgt agaacccaga atcagcagtt
    21721 tttcaccttt tttaaactct aaattgcagt ttctaattgc tggttcatct ttttcaggat
    21781 aggtgcatct aacctcttga aatgttatat cttctatatc tttaggtttt atatcacctc
    21841 gccagttatc ataaaagctg ggaaaatctt ttatctcttc taaacggtct atattaacaa
    21901 acgcttgctt cccggaaaca aacaactggg gcaaatcaag taatggatat atcatcatcc
    21961 ctaaatagga ataaaacata tagaaagttc ctactgatat agtgcccttc actgccatat
    22021 aaccaccaaa aagaatcaca ccaatctgcg ctatataatc aatatagccg taaatcaatt
    22081 ggagaagtgc atttagttta accacgccca tctcagtttt gaatcgttgt gccagtgcct
    22141 tatcaaagaa gcgattatac ttttcttcgc tgacaaaagc tttaacaatc cttatcccag
    22201 aaaaactcat ttccagctga ctattaatat tacttatggc tttctgatta agttcaaaat
    22261 ttcgatagag cttgtccgaa gcaaaataaa acaccaccat cattataggc aaagaagcaa
    22321 tagaaagcaa agtaagtctg acattaatac tgaacattac caccagagca aaaacgatta
    22381 aagagaatga attaactgcc cggaaaattc ctgaacaaag aaaccagctt atcttggaat
    22441 aatccgttaa atcatcggtt aatctggtta caatatcacc tgtcctaaat ttatggaaaa
    22501 acctaaagtc cttggtcaat acgaatttaa aatagtattt tcttaggaaa ttctcaaatc
    22561 taatattcat ccaaccccgg atggcaggat aaaaacctgt gataaattga gcaaagccaa
    22621 gagcaacaaa gacccaaata atacgattta tttcctgcat aggatttgga tatctggcag
    22681 gttctcctaa aatattcttt agtaagtcaa gcaatctacc aaataaaata ggataagcaa
    22741 tggaaactgc tgaacttgcc agcgtaaata ccagcatcaa gagaacaaac cacttttctc
    22801 tcttccattg attaacaatc catttgatgt gcttatccaa gagtgtcctc cagtccaatc
    22861 atctggagag taactaattt tctgtattca ctggattcgt ttaacagttg ttgatgtgta
    22921 cccctatgaa taattctccc tttatcaaaa aagagaatct catctgcatc cagaatagtg
    22981 gttaaacgat gtgccactac tacaacggtt ttattggcga agaccctttc catactatgt
    23041 tgaattctgg cttccgtttg agggtcaacc gaggcagtag cttcatccat aacaattatt
    23101 tcagcatcaa aagccaaagc acgagcaaaa cttattagct gtttttcacc ttgagaaatg
    23161 ttttgacccc gctctgctaa ttcagtatcc aagccaagac gctgttcttg aataaattca
    23221 tctaattgta ctacattaat agcttcttta accttttctt cagatatagc atcgttgtaa
    23281 actctaacat tctctaaaat gctacctgga aaaagaaaaa catcctgcaa aattaaacca
    23341 atctttcttc tccaggctct aaaatcaatc tctgaaagag gaactccatc cactaaaatc
    23401 tgcccctttt gtgcttgata aaaataacac agtagactaa ccgtagtagt ttttccactg
    23461 ccagaaggac caaccagtgc tatcttttta cctttgggta tggtgaaaga gatatccttt
    23521 agtacccaat cttcattatt gtaagcaaac cagacatttt caaagcgaat ctcatgctca
    23581 aaagtaggaa gtctctttcc agtaaaaatc tcatcctccg attgtaattc atttaattcc
    23641 agaattcttt tcaaagagac aaaggaacgc tgaatctgca taacattttc tgagatagac
    23701 attagaggcc aaatcattct ataggtgtaa tcaataaaaa caataagagt ccccagcgtt
    23761 acaatgcctg ccagaatctg aggtgcggtt atccttataa tcattacaat gaaaaagact
    23821 tcaaatagga acataaaaaa actttgagca ccatattcca gataggtggt tttggtctcc
    23881 agtttatatt tatcatccga agcatttctc aaatcattat atactttatt ctgaagattc
    23941 aaggattgaa ttataggcac tccctgaaca tagtctgtta tcttagccgt aaccagagcg
    24001 ttcttttctc ttatctgacg atagaatttg gataaatatt taatcaagta gtaataagct
    24061 ataacaccaa caattatacc aggtaaaata taagcagcca catgccattc ttttaaaaac
    24121 ataactaaaa atattccaat aaagaataat agattgccga taatacggat ggaaagatct
    24181 gagaataaag cttttactct ctctgcatct gattccactc tggctattaa ttctcctact
    24241 gcttgtttat tgaaccattc tactggaaga gttaaaagat gacgaaaaac ctgcgcttta
    24301 aatttcgtga ttactttcac tccaagtttt gaaagtaaaa tagtttgtag ataagtgaga
    24361 gcaccggaca ccaaaactgc tgctataaac attgcaccat aacgatacat ctgagaaaca
    24421 tttcccgaag gaatgctttt atctactata ttggctagta ttaaaggata gactaattgc
    24481 aaagctgaag tgcaaagcat agcaaaaaga ccaaagccta aaagccctat atccttctta
    24541 ataaagggat aaagtaaatg aatataatat ttcagatttt gcttttgatt gggcattcgc
    24601 tcttattacc tgtaattata taattatggt cgggatgaga ggatttggac ctccgacctc
    24661 tcggtcccga accgagcgcg ctaaccggac tgcgctacat cccgaccgtt ttatgcatac
    24721 tttaacttac agactaaatg tcaagagtta attgctaaaa ttatcattat tattaggttt
    24781 tagcaatctt gaaatgtttt ccttactcac ataaaaaagg agagaagata tattcacctt
    24841 ctctccttaa aagataccgc taaaggttaa tatccagcgg ttacccgata aaactgaatg
    24901 ggttcaactc cctctaaatc tactgttact atattatctg ataaatcagt gtcataaata
    24961 gtaataggag taacagttga aagaccctct ggtgtggtag aaacaaaaag atgatagaaa
    25021 agagcatttg aaaccttatt ccagcggagt ttgggaatac cattatcggt ataaatagta
    25081 aaagctgtag gggtatcaat atgtgcttca ccagtactta ttataatatg tctaccgctt
    25141 tctcctgttt ccggatttct gggttgccag gtaccatatt gggtagggtt aatgtttata
    25201 ctattagtag gggaagaatt atagggggta agtgaattat tgtaatacca aaaagcggca
    25261 cctacatcgg aaacaagagt aaaataatta acattgatag agctatccgc catatgttga
    25321 aaatgctgat cctgcgtgcc attcattgtt atttgatagt taaaaatgct gctataatta
    25381 tataaatctc ctgctatggt taagaatatc cagtgagtat taggactatt acagatagtt
    25441 ccataattgt ccaatctttg atttatagta agagtacgat gattaccaga gtagttataa
    25501 accgttgctt g
//
LOCUS       DAORZF010000024        24791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_1682_length_24791_cov_369.293338, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000024 DAORZF010000000
VERSION     DAORZF010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 24791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24791
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1682_length_24791_cov_369.293338"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..1621
                     /gene="proS"
                     /locus_tag="PK015_04195"
     CDS             98..1621
                     /gene="proS"
                     /locus_tag="PK015_04195"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOL33701.1"
                     /translation="MNKAERTAIEPKREENYSEWYQQVIKAADLAENSEVRGCMVIKP
                     WGFAIWENIRTYLDRMFKDTGHQNAYFPIFIPLHYFEKEAEHIEGFAKECAVVTHSRL
                     EADEQGKLKPAGELTEPYIVRPTSETIIGASFARWVNSYRDLPLLINQWANIVRWEMR
                     TRLFLRTTEFLWQEGHTVHETEEDARLETLRMLDVYTKFAEEYLAIPVIKGAKTESEK
                     FPGAVDTYCIEAMMQDKKALQCGTSHFLGQNFARASNIRFQNREGEFQYAWTTSWGVS
                     TRLIGALIMTHSDDNGLVLPPKIAPAHIAIIPIYRNEEEKDKVMSLAHKLSNELRQVN
                     FEGIPILTELDDRDLTSSERNWYWIKKGMPLRIEIGPRDISSNSVMISRRDENPKDKH
                     RIMIEEVVEYCKTTLPEMQKNIFQKAKDFREQNTVKIDSKEEFYRYFTPKNEEEPEIH
                     GGFAHSHWCGNPLCEEKIKEDLKVTIRCIPLESEKEEGKCIYCGQPSSQRVIFAKAY"
     gene            1623..2852
                     /locus_tag="PK015_04200"
     CDS             1623..2852
                     /locus_tag="PK015_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HOL33702.1"
                     /translation="MKKSAVITIGNEILLGKTVNTNLTFLAQQLANLGLPVDYSVTVK
                     DEATAIHQALRQCWEKYDVVITTGGLGPTTDDITKQEIASFFGKKLVFNEEVWEQVQK
                     IFAARNMPTPEINRSQAMVPEDFIALKNERGTAPGLFYEEGEKSFFAFAGVPVEMKHI
                     FLTQAKPILEKKYGTQNKVIQRTIHTFGISESALAELLSDFQPPENVNFAWLPQPGRV
                     DLRFYGTDAQAIEKVFNECYSRVQTYAWGFDDDTPVSVLHHILREKKLYLSVAESCTG
                     GLVQKMVTDLAGSSDVFLGGVVSYANEIKVKILKVKAETLSKYGAVSEECAREMVTGV
                     RDLTDSDIAISTTGIAGPEGGTAEKPVGMVCFGFSVHNDIWSITHIFNGDREIIRQQA
                     AEFALLNLIKYLQGHEF"
     gene            2849..4075
                     /gene="purD"
                     /locus_tag="PK015_04205"
     CDS             2849..4075
                     /gene="purD"
                     /locus_tag="PK015_04205"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOL33703.1"
                     /translation="MKVLIVGSGGREHSLAEAFAKDIKKSDIYVAPGNGGIALDYKCL
                     PLRSKEEIVRWCKENRPELVMVGPEQYLAEGLSNSLNAERIPCVGPSQEAAKIESSKI
                     FAKTLMTKYHIPTASYVAFEDKDQALAYVRYMGKYPLVIKANGLAAGKGVIIAENQKQ
                     AENAIQTLENKVVVEEYLKGWEVSLFVITDGKDFKTTIFSQDHKQLGENDTGPNTGGM
                     GAFAPVKEAEPYRKQIEEQIVAPILEGLKAEGCPYKGFLYCGLMITEEGPKVLEFNCR
                     LGDPETQAILPLLQTSFTEVCYAILNNKVSDLKLEWSPLSSVCVVLASQGYPGIFPTG
                     FPVIINSPITSKIFFAGVENKDGQLITSGGRVLSLVALGKNLSEARRKVYQDINKVNF
                     TGKYFRRDISLRKNKC"
     gene            4091..9109
                     /locus_tag="PK015_04210"
     CDS             4091..9109
                     /locus_tag="PK015_04210"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOL33704.1"
                     /translation="MKKIVLSLFLLIPAIFISAQSRVISQSPNEVTFEFTLPEYQIDY
                     LKINGITWQQIVSDEGIILEKEGYPELLFFCENIAIPVDGDISVKVTSVSSTTLKNIN
                     LKPIEKLVVQDNKVDYQFYQDTKAYRNNQLYPQEIVQAGKSAFIGDRRFVPLHIFPFQ
                     YRATTKELIVNSKFKVEVRILGSKSATPNWQLSENPIDQVGDSFFLNNASSKSWRLAK
                     TKDTNYQSPKGGTSAVNEIQIIVNQEGIYKVTYQQLKDFIQIMTDSLEVAMAWDIDTV
                     DPRHLELTDEKGPVPIHFFGEADGHFNRGDYFEFFGDRHYGDTCYNDDYTAENVYTLY
                     LKSGLGARMAVENGGLVNSNPAQYIVPDAYEQTVHFEQQLLMDKLGHSWSDDNPDFYK
                     EDIWFWRRIKAPDLDIIPFQLQYPKDSVIRRARAKVSLYGLTYSTSLAPGQYDHNATV
                     RINQAMINTHNWIGQTEQIFENDVPFANSYLYHGTNNMYISLSGNTASGTLEQVLLDY
                     LELTYWREYKTSEDKIKFTKPSDRPAGLYQFEISGFNSNDVSVYKIGSSIFNSLQIEP
                     FAVNGGAPWTVSLQDSVYSTDVVYYAVTESKKLSPLGFRLNLPSDLKNPANAANVIMV
                     GNRELLESEGADLLVNIWEADSYVVRKVDYQDIFDEFNHGIRSAESLKEFFTYAYNNW
                     SPPQLNYVVLLGEGINDERDNSPSRQYALVPVKKVWTINHGATASDTWYGTIVGDDPI
                     PDIVITRINAWKPEQVLAYAQKAYNYRNNLLTNRLWNNHITLSAGGTPGSAQNIFALQ
                     SERIRRKSIPQDYRVTRVYGSSQGVNSDYYGSSPQLKEAINTGTQYLQFMGHGGSGIW
                     ADYNLLNLSDIATLNNQTYPIVVSLACYCSAFDTNGLGSISEAFTMQPNKGAIATFGF
                     TGLSYERSDEDWALALTEAIYKHDFPTLGQAYQYTLAKFTSNSGILGITYALTYGATL
                     LGDPLIRLKKPITDISVNTDEAILMPGDTLVVRAQFPQDVLAARLYIQNNDEIILNIP
                     YDLPVINGNYSANYIIPANIQPPYTRKIFLAGYSATDEYIGRSVFGVGRSALTHIKTI
                     PENPAWNDSLSFIAKLFSNEELLSLKATVCVDSTFAGATWLDLPMQAYPDIENGYITI
                     NKLPPLSPGKEYFFKYIMTTNSGNYESVLYSYLTRLPDLYLKDIKLETSNSEQILKVL
                     VQNIGNAASLITDIKLYTQIPNSTETLVKTLDMPPLAVNEEIWVNFSLDDLPQANIGT
                     ITLKVKVNVSGAFPESHSNYNASNVISLTTCFNYYTVTNTGSLIQSVDNNVTCEIPPN
                     LVPSGKNSVFYINSVGTYTANAQPDIYPIKLKSYDNALSIPASNAYEISTLDTALVDS
                     TGTFINNKKIKLTFFYSTNDQETQNLETENSYKIYRWEDSGKKWILQGGNISTAEDKV
                     NFEVHKPGIYTIYRNCDRIRPSIDINVQEQEFTVGGYISGTGTMSLLLSDANGIDVFD
                     NSIQLYLNGNKVPEEDYTVTINSDDLNRIPIKYQLNLSKGTYSLVIDCQDVNGNFNTR
                     ETQFFVNNTFDIKNIGNYPNPVLGVTEDPKNQGRTRFTYVLTDDADEVTIKVYTVAGR
                     LVKTFKNLPTGVGYHEYPRTVYGWDCKDEFGYYLANGVYFYKVIAKKGNKKIEKTMKM
                     AILK"
     gene            9118..10134
                     /locus_tag="PK015_04215"
     CDS             9118..10134
                     /locus_tag="PK015_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33705.1"
                     /translation="MRKTFWILIFTLAFALPLNAGRYAGDFMMIGSGVRPLGMGGAFV
                     SLADDGSAIYWNPSGISQIKKSEVTAMHAFLYNGLASYDHITFCQPLPSDVTIGINIT
                     RLTINDIPLFKESYLIGTNVDQRINNSAYQLTGIPDGKFKSTDDLYQFAFAKHIHYDL
                     NMGWLFFAIPIDFCAGGNIKFINRKIMDNVGSGTGIDFGMKLKTDMAILFDWDALGTL
                     DIGVNFQDIAGTQISWDTPSQTGHSDEVLFNTKLGLAITQPIPKIDSEVILAYDYDYV
                     YNGCNHYGAQFSYKNLGSLRLGYYDTNFTCGATINVYGVNLDYALVTNPVGITNRIGL
                     RVSF"
     gene            10145..10921
                     /locus_tag="PK015_04220"
     CDS             10145..10921
                     /locus_tag="PK015_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33706.1"
                     /translation="MKKYMFLFIIALAIILTGCSGRDENEKDKIPPIPPVLTPHLGDT
                     GDPPVEYGGQLIVLNEDNNGIDAVPDGDWIRVLWNPFKDTDLSHIRIYRFNDFNPEPV
                     FMDSISASAHYYLDTDTNIMERVWYSYFIDLVDLAGNTSRSDTTSYALLPKSILISPA
                     DNSVISPLNMSFKWHRIGSVGKFRIIFFDENYNYVWHKDITTNLENEEFEVIDFPVNI
                     ALQYAGQSLRWRVDSFEYDADKEAFMGSESNERIIYLGQI"
     gene            11047..11622
                     /locus_tag="PK015_04225"
     CDS             11047..11622
                     /locus_tag="PK015_04225"
                     /inference="COORDINATES: protein motif:HMM:NF022007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOL33707.1"
                     /translation="MKKILLFIIITSFTVLAFAQAGNVVVTSTSTSAYSYEGTGAGAQ
                     KLKMNVYILGRVNKPGLYLVPDDTDFPTLLALAGGPAEDAKLSKIRIIRPVNPTLDAE
                     SEPEVIWLDFKKYMDTADNKYLATKTIKTKVIDEFTGEEIEHTVYDLRLQPGDTVVIS
                     GTIFYAFDRIADFLSKVAIALSVYNLIANIK"
     gene            11631..13925
                     /locus_tag="PK015_04230"
     CDS             11631..13925
                     /locus_tag="PK015_04230"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOL33708.1"
                     /translation="MEQQPIQNIQPPQVEEINLKEYLRIIYQFRYLIVIIFVVVIIGT
                     ILYTYQQPRIYSASSKILLENQTTGADILMLTGQGSGKNYINNQIQLIKSKPLLSAAW
                     EIMKKNPEWEAYPISSTENPIGHLGGIKVEALRETDILTLSYESTNPGEAMAVVNAIA
                     DAVVQQNNQIARLEYTTVREFLGEQLDLISRRLQASEEDLRAFKNENKIVELSETTKK
                     WIEQSADIESQYESALTDQAVKARTLEVLKKQLQDQDSLMVEVDNVLRAPYIEQLRKD
                     ITNTQATIAKLRTNENYSSDHPQIKLLQEELSKTKATLDAEIKKYISKNLNYDPLSFR
                     NQILTKIVQAEVDLEMARTQVQGLEQTKAIFEDRIITLPDKELQLARLTRSMALDEKI
                     YGIMMEKYEDARIAEQAKIGNIRVIDYATRPGKPIKPNTSMNLLVGILLGLGLGVATA
                     LIVHSMDTKLRTLEDIESYVRLPIMGTIPLIQESESRIIEFNQMIEQSEGENKEQLSK
                     SLHYVMMQLVSHYAPKSPISEAYRTLRTNILSKKPEGSTSILITSPGPKEGKSTTISN
                     LAITFSQMNAKVILVDLDMRRPMLHNKFALEKENGISDYLIDPDVPLKQIIKASGIPN
                     LDIITSGFIPPNPSELISSPRTDKLIEELKSLYDYILFDTPPVIAVTDALILTKKVDL
                     TFFVVRCGTTEKGIVKRARDMMENVNANIDGIIVNGVYVQKYYSKSNYYYYYYYYYYY
                     YGEEKPKKQKKANLSFIRKNKSIP"
     gene            13852..14730
                     /gene="ispE"
                     /locus_tag="PK015_04235"
     CDS             13852..14730
                     /gene="ispE"
                     /locus_tag="PK015_04235"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOL33709.1"
                     /translation="MVKKNLKNKRRLIYPSYAKINLFLEILGSLPNNFHQIETLLCSV
                     SLFDTIKYTLTKNKDSKKWIYLNDKINKNNLIYKVAEYLYNQYKPDWGIEIQLIKRIP
                     IAAGLGGGSSNAATTLLALNSLWELGLSIDELNSIAASFGSDIPYFLYGGTALATNRG
                     EIIQPIEDFKLDNILLVNPNIMISSSEAYRIASIPAENERRQCIISQDVNWFFNRLEV
                     QVRMDYPLVNEILDDLIKFGASTALMSGSGPTCFGVFEERLKMQKCEGYFKEKGYWTK
                     VVRTISKEEYQNVFQA"
     gene            14714..15658
                     /locus_tag="PK015_04240"
     CDS             14714..15658
                     /locus_tag="PK015_04240"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOL33710.1"
                     /translation="MYSKLKLISGHANRPLAEEVANYLGIPLSDIDLFKFSNEESFVK
                     INESVRGADVFIIQPTCRPVNDNLIDLLIMIDALKRASAQRINCVIPYYAYARSDKKD
                     QPRVPITAKLVADLLTVTGANRVVAVDLHAEQIQGFFSIPVDHLYTIPIFARYFNEMN
                     LDNPVVISPDSGGANRARALAKRLNCGLAIGDKRRQANDDKTELLNIIGDVEDKTAIL
                     YDDIIDTGSSLIKIANVLSTKNVKNIYAACTHGVLSCSAVQNINNSNIEKLFITNTIP
                     LSEEAAACPKIIQLSIAEMLAMAIKKIHTEESLSVLFT"
     gene            15682..16290
                     /locus_tag="PK015_04245"
     CDS             15682..16290
                     /locus_tag="PK015_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOL33711.1"
                     /translation="MIFTIEAQKRTNTKHSELTALREQGLIPAVIYGKALESTPIAVK
                     RNEFLKCYKKSFQELSFYEIILDGQKYHTILKDKQIHPVSRDFLHLDFMLVEATAMME
                     FEIPLQFVGEPIGVKTGGFLETLQRTVKISCKAEDAPEEITIDISGMQIGDSLHIKDL
                     PQGNWQYKDNPDTALLVVLAQRVEEAEAPKEAEEVSTEEKTE"
     gene            16290..16880
                     /gene="pth"
                     /locus_tag="PK015_04250"
     CDS             16290..16880
                     /gene="pth"
                     /locus_tag="PK015_04250"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOL33712.1"
                     /translation="MKLVLGLGNIGKDYEGTRHNIGFMCLDTWAKKHNLTFRSNQLYY
                     FIQYKNASLIKPKTFMNRSGLALKEASLKWNCSEALVIYDDIELPLASLRVRTGGGDG
                     GHNGIKSLLEIFPPENLKRIRIGIGRSEENPRDYVLDKFTQEELSALMPAIELACDFI
                     DIYIDKDFYAVLDAYSIWKKTFSVNNVHGNDSPKEK"
     gene            16881..17171
                     /gene="rpsF"
                     /locus_tag="PK015_04255"
     CDS             16881..17171
                     /gene="rpsF"
                     /locus_tag="PK015_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOL33713.1"
                     /translation="MKNYELMVIISPKLTEEEANKLNESILNMVKEQGNGEIIKTDPW
                     GLRKLAYPINKMQEAYYFVNYFSMEPSAVKPIKNQLNINEHVLRHMFIVRDK"
     gene            17180..17626
                     /locus_tag="PK015_04260"
     CDS             17180..17626
                     /locus_tag="PK015_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOL33714.1"
                     /translation="MADLRLPRLNKVFIAGRITQEPELRYTPKGTPVVRFTVATDRRY
                     KDEMDQWQSMPMYIDVIAWSNQAENICKIAHKGTAVLVEGKIDTRSYVDSNNNNRKVF
                     EIIADHIQSLEWTPKAEGEGVEEEVPLPQEEPNYVAEPVTDDDVPF"
     gene            17642..17878
                     /gene="rpsR"
                     /locus_tag="PK015_04265"
     CDS             17642..17878
                     /gene="rpsR"
                     /locus_tag="PK015_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOL33715.1"
                     /translation="MNTMDRKKKPAHKKYCRFCANKDLVIDYKNVELLSQFISDVGKI
                     ESSRLTGTCAKHQRKLSKEIKRARQMALLPYVIE"
     gene            17878..18714
                     /locus_tag="PK015_04270"
     CDS             17878..18714
                     /locus_tag="PK015_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33716.1"
                     /translation="MPFIALLLGALATLNPFIGLLVMMIYSHSFFRLLTGKPVNRALM
                     FLVFPVIVAIFTFNNPSTLIYPVDAIFGAGLVVILYLYALIKRGNPVTALINAALLIT
                     VYGVIRYYLFGSFLLNTLEQSFAEMEKIMPQAMQNGNITQMKPMLQILLPASWTVSQV
                     LALSAGHLIFLHLGGTHSELRDFKLPAYYNLFIIAVLPLYFFPQLHSVFINVLLALCV
                     LPMVEGIGVIINLISKRKPNILINILVTILVLLNLPIMALIGFADIWLDFRKLNIKGN
                     LS"
     gene            18711..19241
                     /gene="rplI"
                     /locus_tag="PK015_04275"
     CDS             18711..19241
                     /gene="rplI"
                     /locus_tag="PK015_04275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOL33717.1"
                     /translation="MKVILLNPIEKLGNKGDVVNVKRGYARNYLIPRKLAIYATPQNL
                     KNWNSIQTKAAEEEEKMIDAMKKLDEQLRNMTLHFERKTDEQNNLYGSVSEMDICNEL
                     AKHGINIKCAMVKMDRHIKSLGESLVKIHLYKDIFSELKVVVDKEGSGQKNIEPKIDN
                     SAPETSDEAESEITTD"
     gene            19261..20013
                     /locus_tag="PK015_04280"
     CDS             19261..20013
                     /locus_tag="PK015_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33718.1"
                     /translation="MKKYMYQISRILAFVAMLVAVSMLSIGNPKNIPFFILLFAAIFA
                     LVYYLTKRSEENQRLGKAGTGTKPITQLLTVVLFVALISILGLLRHGILGFIGWFLVI
                     GGIFSLIYLSVRKHQRHFEIITSTPKNTKVTGIILAIISILLPVIVISFTNIVPIAKA
                     SLALSIVLSLIGTLAFVVLICLGLYLINIIGNKTSNLILGYLAIILAALLPGIILLFT
                     SATTTACAVIYIIAIITALLAYFAFNQLVTNK"
     gene            20052..20339
                     /locus_tag="PK015_04285"
     CDS             20052..20339
                     /locus_tag="PK015_04285"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOL33719.1"
                     /translation="MAFTSLSNIKKEVIDRIAGEKYKSFVNLYQHWKDIVGPLLAARS
                     HPFHLKDSILYVAVENNSWLQELVLRKMDILKQLNDKTGEEIKDIIFLIKT"
     gene            20344..20991
                     /gene="rpe"
                     /locus_tag="PK015_04290"
     CDS             20344..20991
                     /gene="rpe"
                     /locus_tag="PK015_04290"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOL33720.1"
                     /translation="MKPIQIAASVLSCDFAHLAKELSRASDANMFHLDIMDGHYVPNL
                     TFGQPIVLKIRQLTKIPLDAHLMVTNPENYVAPFASIGVNWFSFHQECVYHSHRVVQQ
                     IKSLGMKAGIALNPATPLSTLDCILPDLDFVLLMSVNPGFSGQEFIPSVLEKIIQLKD
                     IITKNSLNTLIEVDGGINETNASLVIAAGADILVSASYIFGSTDYSAAIRSLRGS"
     gene            21079..22410
                     /gene="ffh"
                     /locus_tag="PK015_04295"
     CDS             21079..22410
                     /gene="ffh"
                     /locus_tag="PK015_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOL33721.1"
                     /translation="MFDSLTDRLDKVFRNLRGKGYLTEQNIKDALREIRIALLEADVN
                     YRIVKNFISEVEKRAIGEEVMKSLTPGQQVVKIVYDQLVALMDTEGFELKVYNNKLTK
                     VMLVGLQGSGKTTACAKLALYYRKKGIKPMMVACDVYRPAAIEQLKILGKQIDIPVVS
                     EDTKDVYKIAEKALNLASQQLINLLIFDTAGRLQIDEPMMDELVRLKAQIKPDYILFV
                     ADATTGQEAVNVAKEFYDKLTFDAVILTKLDGDARGGAALSIKAVTDRPIAFVGIGEK
                     ISDLEVFYPDRMASRILGMGDVLTLIEKAEQAIDEKESEKLAKKMLKNQFTLNDFLKQ
                     LQNIKKMGSLESIIRMIPGLGHKLPADLKIDDKAFKHIEAIIQSMTEKEREHPEIING
                     SRRLRIAKGSGQSVTEVNRLLRQFDEMKKMMKRFSSPQGKRDLENMMKRSG"
     gene            complement(22760..23491)
                     /locus_tag="PK015_04300"
     CDS             complement(22760..23491)
                     /locus_tag="PK015_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOL33722.1"
                     /translation="MSELRFSRTALLVGSEAMARLNNSRVAVIGLGGVGSYAVEALAR
                     AGIGHFTLVDFDVVGESNFNRQLHALNHTLGEPKTEVMKKHIEDINPAAEVITYPVFL
                     DSTNREEILKEQDFYLDAIDSLGPKCGLLEYAVKNNFKIISVMGSGNRLNPEEIHLAP
                     LNETVNCPLARRVRKHLKRWGVDCNFPCVYSSELPKKIFKDISSPDEDLIHRGRERGK
                     VGTISYMPAIMGMMAASWIIRQIVE"
     gene            complement(23488..24222)
                     /locus_tag="PK015_04305"
     CDS             complement(23488..24222)
                     /locus_tag="PK015_04305"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOL33723.1"
                     /translation="MNLVDGHCHLASLNQIMPVESLLKEANEAGITHFLSSALSKEEI
                     AFHRQYSLPGVLFSAGIHPHYAECDLELKDIEELCAEKCIWAVGEIGLDRYGKDIERQ
                     KQLLSQQLELANQYQLPVVLHIVGYQQQAYELLKLYPLVYLVHGYAGSLEGYRILSRL
                     NSYFTISERILRPDKYNLLQEMVKDKRYLLETDITKDYVLPGEHNPLLRLVDVFEQVQ
                     KQCRVDAEEILTTQYENFIALTQGKL"
     gene            complement(24219..>24791)
                     /locus_tag="PK015_04310"
     CDS             complement(24219..>24791)
                     /locus_tag="PK015_04310"
                     /inference="COORDINATES: protein motif:HMM:NF016357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HOL33724.1"
                     /translation="PKLDPYYNFKKLKPSNKPIIVFTATYDKSGMSAIHLWADKLGLL
                     TGKYDIYVTLHPWMSRKYVKIIKNTEGVKLIEEESPLKYIAESDVCIGDTSSILAECC
                     ALDKPIITWKVPTSERTVPEIEEILARCTWQIATFEELEPAIEHCLAHPEELQEERKE
                     ASAIFFDELDGKAGFRVANEILKILPELKK"
BASE COUNT         8060 a         4449 c         4667 g         7615 t
ORIGIN      
        1 tcctaaaata taattttaac gctatgcaaa agttttctta ttgacaatat gtgagtatta
       61 aaggaattgt gctaatataa aaagtaaggg atgttcaatg aacaaagcag aacggacggc
      121 aatagagcct aaaagagaag aaaattattc agaatggtat cagcaagtga ttaaagctgc
      181 tgatttagca gaaaatagtg aagtacgtgg ttgtatggtc attaagccct ggggttttgc
      241 tatttgggaa aatataagaa cctaccttga ccgtatgttc aaagatacag gtcatcaaaa
      301 tgcctatttc ccaatcttta ttcccttaca ttactttgaa aaagaagcag aacatattga
      361 aggtttcgcc aaagaatgtg ccgtagtgac acatagcaga ttggaagcag atgagcaggg
      421 aaaacttaag ccagcaggtg aattaactga accctacata gttcgtccta ccagtgaaac
      481 aattattgga gcttcctttg ctcgctgggt caattcctat agagaccttc ctctcctcat
      541 aaatcaatgg gcaaatattg tcagatggga aatgcgaacc cgattgttcc ttcgcactac
      601 ggaatttctc tggcaggaag gacatactgt ccacgaaacg gaagaagatg caaggttaga
      661 aactttacga atgctggatg tttatactaa atttgcggaa gaatatcttg ctattcccgt
      721 gattaaaggt gccaagacgg aaagtgaaaa atttcctgga gcagttgata cctattgcat
      781 tgaagctatg atgcaagata aaaaagcgtt acaatgtggc acttctcact ttttaggaca
      841 gaactttgcc cgtgcttcca atatccgttt ccagaataga gagggtgaat ttcaatatgc
      901 ctggacaacc tcttggggtg tttctactcg tcttataggt gctttaatta tgactcatag
      961 cgatgataat ggactggtgc ttcctcccaa aattgctcct gctcatatcg ccatcatacc
     1021 tatctatcgc aatgaagaag aaaaagataa ggtgatgtca cttgctcata aactaagtaa
     1081 tgaactgaga caagttaatt ttgaaggaat acctattcta actgaactgg atgaccgtga
     1141 ccttacctcc agtgaacgaa actggtactg gattaaaaag gggatgccct taaggataga
     1201 aattggtccc cgagacattt cctctaatag cgtgatgata tccagaagag acgaaaatcc
     1261 caaagataaa catcgtatta tgattgaaga ggtagtggaa tattgcaaga ccacccttcc
     1321 agaaatgcaa aagaatatct ttcagaaagc aaaagacttt cgtgaacaaa acacagtcaa
     1381 gatagatagt aaggaagaat tctatcgcta tttcactcct aaaaatgaag aagaaccaga
     1441 aattcatggt ggatttgccc attctcattg gtgtggcaat cctctctgtg aagaaaaaat
     1501 aaaggaagat ttgaaggtaa ctatacgttg cattcctctt gaatcagaaa aagaagaagg
     1561 gaaatgcatt tattgtggtc aaccatcatc tcagcgagta atttttgcta aggcatatta
     1621 agatgaaaaa aagtgctgta ataaccatcg gtaatgagat tttactgggt aagaccgtta
     1681 ataccaatct cactttcctg gctcagcaat tggctaattt aggtcttcca gtagattatt
     1741 cggtaaccgt taaagacgaa gctacagcca ttcatcaagc tttgagacaa tgctgggaaa
     1801 aatatgatgt tgtcatcacc actggaggtt taggacctac tacagacgac ataactaaac
     1861 aagagatagc ttccttcttc gggaaaaaat tggtctttaa tgaagaagtc tgggaacagg
     1921 tgcagaagat ttttgccgca agaaatatgc ctactcctga aataaatcgt agtcaagcta
     1981 tggttccgga agattttata gctttaaaaa atgaacgagg cactgctcct ggcttattct
     2041 atgaagaagg agaaaagagt ttcttcgctt ttgctggcgt cccggtagag atgaaacata
     2101 tcttcttaac tcaagcaaaa ccaatcttag aaaagaaata tggcactcaa aataaagtta
     2161 ttcaaagaac tattcacact tttggcatat cagaatctgc cttagcggaa ctgttatccg
     2221 atttccaacc acctgaaaat gttaactttg cctggcttcc tcaaccagga agagtagatt
     2281 tgagatttta tggaactgac gctcaggcta ttgaaaaagt tttcaacgaa tgctattcac
     2341 gagttcaaac atatgcctgg ggatttgatg atgacactcc cgtaagcgtt ttacaccata
     2401 ttttaagaga gaaaaaatta tacctttccg tagcagaatc ctgcactgga ggtctcgttc
     2461 aaaaaatggt cactgactta gctggctctt ccgatgtatt tttaggtgga gtagttagct
     2521 atgccaatga aatcaaagtg aagatattaa aggttaaagc tgaaacactt agcaaatatg
     2581 gtgcagtgag tgaagaatgt gcacgggaaa tggtcactgg tgtaagagac ttgactgatt
     2641 cagatatagc catttctacc acaggaattg ccggacctga gggtggtact gcagaaaagc
     2701 ctgtaggaat ggtctgtttc ggtttttccg ttcataatga catctggagt ataactcata
     2761 ttttcaatgg agatagagaa atcattcgtc aacaagcagc tgagtttgct cttctgaatt
     2821 taatcaaata cttacaaggt cacgaatttt gaaggttttg attgtaggca gtggagggcg
     2881 tgaacattcc ttagcagaag cttttgctaa ggatataaaa aaatcggaca tctatgttgc
     2941 tcctggtaat ggaggaattg ccttagatta taaatgttta cctctgcgta gtaaagaaga
     3001 aatcgtaaga tggtgcaaag aaaatcgtcc tgagctggta atggtaggac cagaacaata
     3061 tctggcagaa ggattatcta atagcctgaa tgccgaacga atcccctgtg tgggtccttc
     3121 tcaagaggca gcaaaaattg aaagcagtaa gatttttgct aaaaccttga tgactaaata
     3181 ccacattcct accgcttctt atgtggcatt tgaagataaa gaccaggctt tagcttatgt
     3241 gcgttatatg ggcaaatatc ctctggtgat aaaggcaaat ggacttgccg ctggaaaagg
     3301 tgttatcatt gctgaaaatc aaaaacaagc agaaaatgct atccaaacac tggaaaataa
     3361 agtagtggtt gaagaatact taaaaggctg ggaagtttct ttattcgtga taacagatgg
     3421 taaagatttt aagactacca tcttttctca agatcataaa caattgggtg aaaatgatac
     3481 aggacctaat acaggtggaa tgggagcttt tgctccagtt aaagaagctg aaccctatcg
     3541 taaacaaata gaagagcaaa ttgttgcacc tattctggaa ggattaaaag ctgaaggatg
     3601 tccctacaaa ggttttcttt attgtggttt gatgataact gaagaaggac ctaaggttct
     3661 ggaattcaat tgccgtctgg gagaccctga aactcaagca attcttcctt tactacaaac
     3721 ttctttcacc gaggtctgtt atgctattct gaataataag gtttcagatt taaaactgga
     3781 atggagcccc ttaagcagtg tttgtgtggt tcttgcttct caaggttatc ctggcatttt
     3841 tcctaccggt tttccagtta ttattaattc tcctatcacc agtaagatat tctttgctgg
     3901 agtagaaaat aaagatggac aactgattac ttctggagga agagttcttt ctctggtagc
     3961 tctgggcaaa aacctctcgg aagctcggag aaaagtctat caagatataa ataaagttaa
     4021 ttttaccgga aaatacttcc ggagagatat ctctttacga aaaaataagt gttaaggtta
     4081 aaagatattt atgaaaaaaa tagttttgtc cttgttttta cttatcccag caatatttat
     4141 tagtgctcag tcacgagtta tcagccaaag tccaaatgag gtaacttttg aatttaccct
     4201 tcctgaatat cagatagatt atttgaagat aaatggaatc acctggcaac aaatagtctc
     4261 tgatgaagga attattttag aaaaagaagg ttatcccgaa ttattattct tctgcgaaaa
     4321 tatagctata cccgtggatg gagatatctc ggttaaagtt accagtgtct ccagcactac
     4381 tttaaaaaat ataaatttga aacctataga aaagttggtg gttcaagata ataaggttga
     4441 ttaccaattt tatcaggata ctaaagccta cagaaataat cagctatatc ctcaggagat
     4501 agttcaagct ggtaaaagtg ctttcatagg agaccgcaga tttgttccac tacatatctt
     4561 cccttttcag tatagagcta caacgaagga attgattgtt aatagcaaat ttaaagtgga
     4621 agttcggatt ctgggaagta aatctgccac acctaactgg caattgagtg aaaacccaat
     4681 tgaccaggtt ggcgattcct ttttcctgaa taatgcttca tccaaaagct ggcgtttagc
     4741 taaaactaaa gatactaatt atcaatctcc taaaggtgga acaagtgcag ttaatgaaat
     4801 ccagattatt gtcaatcagg aaggaattta taaagtcacc tatcagcaat tgaaagactt
     4861 tatccagatt atgactgatt ctttagaagt ggcaatggca tgggatattg atacagttga
     4921 ccctcgtcac ttagaattaa ccgatgaaaa aggtcctgtc cccatccact tttttggtga
     4981 agcggatgga catttcaata gaggagatta ctttgaattc tttggagacc gtcattatgg
     5041 tgatacctgc tataatgatg actatacagc cgaaaatgtg tacactcttt atctgaaatc
     5101 tggattaggt gccagaatgg cggttgaaaa tgggggttta gtcaactcca atcctgctca
     5161 atatatagtt cctgatgcct atgagcaaac tgtccatttt gaacaacaat tattaatgga
     5221 taaattagga catagctggt cagatgacaa tcctgatttt tataaagagg acatctggtt
     5281 ctggagaaga attaaagctc cagatttgga tatcattccc ttccagctac agtatcccaa
     5341 agatagcgtt ataaggcgtg ctcgtgcaaa ggtttcacta tatggtctaa cttattccac
     5401 ttctcttgca ccaggacaat atgaccataa tgctacggtg agaataaatc aagctatgat
     5461 taatactcat aactggattg ggcaaaccga acaaatcttt gagaatgatg tgccttttgc
     5521 caattcttat ctttatcacg gaacaaataa tatgtatatc agtctttctg gtaatacagc
     5581 atctggaacc ttagaacaag ttctcctgga ctatttagaa ctcacctact ggcgtgaata
     5641 taagaccagt gaagataaaa taaagttcac caaaccatca gaccgtccag caggtttata
     5701 tcagtttgaa ataagtggtt ttaatagtaa cgatgtctca gtttataaga taggctcaag
     5761 tatttttaat agcctgcaaa tagaaccttt tgcggttaat ggaggtgctc cctggactgt
     5821 ctcactacag gatagtgttt attctaccga tgtggtttat tacgctgtca cagaaagtaa
     5881 aaaactatct cctttggggt tccggttaaa tctaccgtcg gatttaaaga atcctgctaa
     5941 tgcggcaaat gttataatgg taggtaatag agagttactg gaatcagaag gagcagatct
     6001 cttagttaat atctgggaag cagatagcta tgttgtcaga aaagttgatt atcaggacat
     6061 cttcgatgaa tttaatcacg ggataaggtc tgcggaaagt ttaaaagagt ttttcaccta
     6121 cgcctataat aattggtctc ctccccagct caattatgtt gtcttattag gtgaaggtat
     6181 aaatgatgag cgagataata gtccctctcg tcaatatgct ttagttccag ttaaaaaagt
     6241 atggaccata aatcatggtg ctactgccag tgatacctgg tatggaacaa ttgtaggaga
     6301 tgaccccatt ccagacattg taataactcg gataaatgcc tggaaaccag aacaggttct
     6361 tgcctatgct caaaaagctt ataattacag aaataatctt ctcactaata ggttgtggaa
     6421 taatcatatc acattaagtg ctggaggtac acctggttca gctcaaaata tttttgcctt
     6481 gcaatcggaa cgcataagaa gaaagagcat tccacaagat tacagagtga ccagagtcta
     6541 tggttcttcc cagggtgtaa attcagatta ttatggcagt tcacctcaat taaaagaggc
     6601 aattaatacc ggaactcagt atttacaatt tatgggacat ggtggttctg gtatttgggc
     6661 agattacaat ctgctaaact taagcgatat agccacatta aataatcaaa cttatcctat
     6721 tgtagttagc ctggcttgct attgctctgc ttttgatacc aatggattag gaagtatcag
     6781 tgaagccttt actatgcaac caaataaggg agctattgct acctttggtt ttacgggatt
     6841 aagttatgaa cgctctgatg aggattgggc tttagcctta actgaagcaa tttataaaca
     6901 cgatttccca actcttggtc aagcttatca atacacttta gctaaattta ctagtaatag
     6961 tggaatttta gggataacct atgccctaac ctatggagct actcttttag gagacccctt
     7021 aattagattg aaaaaaccta taactgatat ctcagttaat acagatgaag ctattttaat
     7081 gccaggtgac accctggtcg tgagagcaca attccctcag gatgttcttg cagcaagatt
     7141 atatattcag aataacgatg aaattatttt aaatataccc tatgatttac cagtaataaa
     7201 tgggaactat agtgccaact atatcattcc agctaatatt cagcctcctt atacccggaa
     7261 gattttcctg gctggatatt ctgcgactga tgagtatata ggcaggtctg tttttggagt
     7321 aggtcgttct gcattaactc atattaaaac tataccggaa aatcctgcct ggaatgattc
     7381 tctttctttt attgctaaac ttttcagcaa tgaggagctg ctttccctaa aagctactgt
     7441 ttgtgtggat agcacttttg cgggtgctac ctggttagac ttaccaatgc aagcctatcc
     7501 agatattgaa aatggttata ttaccataaa caaattacca cctctttctc ccggaaaaga
     7561 gtacttcttt aaatatataa tgactaccaa tagcggtaac tacgagtccg tgttgtatag
     7621 ttatttaacc cgattaccag acttgtattt aaaagatatt aagttggaaa catcaaattc
     7681 tgagcaaata cttaaagttc tggtacaaaa tataggcaac gctgcttctt tgattaccga
     7741 tatcaaactc tatactcaga ttcctaatag cacagagaca cttgtaaaaa ctctggatat
     7801 gccaccactg gcagtgaatg aagaaatatg ggttaatttt tccttagatg accttccaca
     7861 agcaaatata ggaacaataa ccttaaaagt aaaagttaat gtttctggag ctttcccaga
     7921 atcacactcc aactataatg caagcaatgt aatctcatta actacctgct tcaactatta
     7981 tactgtcact aatactggct ctttgataca gagtgtggat aataatgtta cctgtgaaat
     8041 accgccaaat ctggttccaa gcgggaaaaa ttctgtattc tatattaata gtgttggaac
     8101 ttatacagct aatgctcagc cagatatata tccaataaaa ttgaaaagtt atgacaatgc
     8161 actctctatt cctgcttcca atgcatatga aattagcacc ctggatactg ctctggtgga
     8221 ttccaccgga acatttataa ataataaaaa gataaagctt acctttttct atagcacaaa
     8281 tgaccaggaa acacagaatc tggaaacgga aaattcctat aaaatctatc gctgggaaga
     8341 ttctgggaaa aaatggatac tacaaggcgg gaatatttca accgctgagg acaaggtcaa
     8401 ttttgaagta cataaacccg gtatctatac catttatcgt aattgtgacc gaataagacc
     8461 ttcaatagat atcaatgtgc aagaacagga atttaccgtc ggaggctata tatctggaac
     8521 tgggactatg tcactattac taagtgatgc taatggaatt gatgtgtttg acaattcaat
     8581 ccagctttat cttaatggaa ataaagttcc agaagaagat tacaccgtca ccatcaactc
     8641 cgatgattta aatagaattc ccataaagta tcagcttaat ttaagcaaag ggacctattc
     8701 actggttata gattgtcagg atgtaaatgg caatttcaat actcgagaaa cacaattctt
     8761 tgtaaataac acctttgata ttaaaaatat cggcaattat cctaacccag ttctgggtgt
     8821 cacagaagac cctaaaaatc agggtagaac acgttttacc tatgtgttga ccgatgatgc
     8881 agatgaagtg accataaagg tttataccgt agcaggtcgt ctggttaaga cctttaaaaa
     8941 tctccccact ggagttggat accatgaata tcccagaacg gtttatggat gggattgtaa
     9001 agatgagttt ggatattatt tggctaatgg agtctatttt tacaaggtta tagccaaaaa
     9061 gggaaacaaa aagattgaaa aaactatgaa aatggctatt ttgaaatagg tgcagatatg
     9121 agaaagactt tctggatatt aatttttact ctcgcttttg ctttaccttt aaacgctggt
     9181 cgctatgctg gtgattttat gatgataggt tcgggtgtgc gtcccttagg aatgggaggt
     9241 gcttttgtct ctttggcaga tgatggctct gcaatctact ggaatccttc gggtatatca
     9301 caaataaaaa aatctgaggt tactgctatg catgcttttt tgtataatgg tttagcctct
     9361 tatgaccata ttactttttg ccaacccttg ccaagtgatg tgactattgg aataaatatc
     9421 actcgcttaa ccataaatga tatacccctc ttcaaagaat cttatttaat cggaactaat
     9481 gtagaccaga ggataaataa tagtgcctat caattaacag gaattcctga tggaaaattt
     9541 aagagcactg atgacctcta tcaatttgcc tttgccaaac atattcatta tgatttaaat
     9601 atgggatggc tctttttcgc tattcctatt gatttctgtg cgggtggaaa cattaagttc
     9661 ataaatagaa aaataatgga taatgttggt tcaggcacgg gaattgattt tggtatgaaa
     9721 cttaaaaccg atatggcaat actctttgat tgggacgcct taggaacttt agatatagga
     9781 gttaatttcc aggatattgc tggaactcaa atcagctggg ataccccttc acaaactgga
     9841 catagcgatg aagttttatt caatactaaa ttaggacttg ccataactca acctatccca
     9901 aagattgatt cagaggttat tctggcatat gattatgatt atgtctacaa tggatgcaac
     9961 cattatggtg cccaattttc ttataaaaat ttgggaagtt tgagattggg ctactatgac
    10021 accaatttca cctgtggggc aaccattaat gtctatggag ttaatcttga ctatgcattg
    10081 gtcacaaatc cagtaggtat aaccaataga attggtttga gagttagctt ttagtggaga
    10141 taaaatgaaa aaatatatgt ttttatttat tatagcgttg gctatcatct taaccggatg
    10201 ttctggtaga gatgaaaatg aaaaagataa aatccctcct atcccgccag ttttaactcc
    10261 tcatttaggc gacactggag atccacccgt agaatatgga ggacaactga ttgttcttaa
    10321 tgaggacaac aatggaattg acgcagttcc cgatggcgat tggataagag tgctctggaa
    10381 tcctttcaaa gatactgatt tgagccatat aaggatttat cgttttaatg attttaaccc
    10441 tgaacctgta tttatggatt caatatctgc ttccgcacat tactatctgg atacagacac
    10501 caatataatg gaacgagtgt ggtattccta ctttatagac ctggtagatt tagctgggaa
    10561 tacatctcgt tctgatacca cttcttatgc tctattacct aaaagcattc ttatttctcc
    10621 agcagataat tctgtaatat ctcctttaaa tatgagtttc aaatggcatc gaattggtag
    10681 tgtgggcaaa tttagaatta ttttctttga cgagaattac aattatgtct ggcataagga
    10741 catcactaca aacttagaaa atgaagagtt tgaggttata gatttccccg tcaatattgc
    10801 tctgcaatat gcaggacaaa gtttgagatg gcgagtagat tcatttgaat atgatgcaga
    10861 caaagaagcc tttatgggtt cagaatcaaa tgaaagaatt atctatcttg gtcagatatg
    10921 acagatatga accgtactat tctaatttca aagaatatga ttaggtctac aatgaaatta
    10981 atattttatc ttgacataaa tctataaatg aagaaaatta attaaattct aataaatgag
    11041 gctaaattga agaaaatact gctctttatc ataatcacca gctttacagt attagctttt
    11101 gctcaagcag gaaatgtagt ggttacttca accagcacct ctgcttattc ctatgaggga
    11161 actggagcag gagcacaaaa attgaaaatg aatgtttata ttctcggtag ggtcaataaa
    11221 ccaggtttat atctagtccc cgatgataca gattttccta ctttgttagc tttagctgga
    11281 ggtccagcag aagatgcaaa actttctaaa atccgtatta ttagaccagt taatcctact
    11341 ttagatgcag aatcggaacc agaagttatc tggctggatt tcaaaaaata tatggataca
    11401 gctgataata aatatcttgc cacaaagacc ataaaaacga aagttattga tgaatttaca
    11461 ggagaagaaa tagaacatac tgtttatgac ctgcgtcttc aacctggaga tacagttgta
    11521 atctctggca ctatctttta tgcttttgat agaatagcgg actttctctc caaagttgct
    11581 atcgcactta gcgtttacaa cttaatagcc aatattaagt aggtgataag atggaacaac
    11641 aacctataca aaatatccaa ccaccccaag tggaagagat aaatctcaaa gaataccttc
    11701 gcattattta tcaattccgc tatttaatag ttattatatt tgttgtagtt atcattggaa
    11761 ctattcttta tacttatcaa caaccccgta tttactctgc ttcttctaaa attttattag
    11821 aaaatcagac taccggagca gatatcttaa tgttaactgg tcaaggtagt ggaaaaaact
    11881 acataaataa tcagattcag cttatcaaga gtaagccact tttatctgct gcttgggaga
    11941 taatgaaaaa gaatcctgag tgggaagctt atcctataag ctccacggaa aatccaatcg
    12001 gtcacctggg tggtataaaa gtagaagcct taagagagac agatatactg actctttcct
    12061 atgaatctac caatccaggt gaagcaatgg ctgttgttaa tgctatcgct gatgccgtgg
    12121 tacaacaaaa caatcagata gcaaggttgg aatatactac tgttagagaa tttctgggag
    12181 agcaattaga cctgatttct cgtagattac aagcctccga agaggattta agagctttta
    12241 aaaatgaaaa taagatagtg gaactatctg agaccaccaa aaaatggatt gagcaatctg
    12301 cagatataga atctcaatat gaatcagcat taacagacca ggcagttaaa gcaaggactc
    12361 tggaagtgtt aaagaaacag ttacaagacc aggattcttt aatggtagaa gttgataatg
    12421 tcttaagagc accttacata gagcagttac gtaaagatat aactaacact caagccacta
    12481 tagcaaaatt acgcactaac gaaaattatt cttccgacca tccccaaata aaactccttc
    12541 aagaagaact ttctaaaact aaggctactc tggatgctga aataaaaaaa tatatcagta
    12601 aaaatttaaa ttatgacccc ctctctttca gaaatcaaat ccttactaag atagttcaag
    12661 cagaagtaga ccttgaaatg gctcgcactc aagttcaagg actggaacaa acaaaagcta
    12721 tctttgaaga tagaattatc actttgccag ataaagaact tcagctagca cgtttaacca
    12781 gaagtatggc tctggatgaa aaaatttacg gcataatgat ggaaaagtat gaagatgccc
    12841 gaattgcaga acaggcaaaa ataggtaata taagagtcat tgattatgcc acaagaccag
    12901 gtaaacccat taaacctaat acctctatga atttattagt tggtatcctg cttggtttag
    12961 gattaggtgt tgctacagca ttaattgtac attctatgga tactaaattg cgtactcttg
    13021 aagatataga aagctatgtg cgtctgccta ttatgggaac tattcctctg attcaagaat
    13081 ctgaatcccg gattatagaa ttcaaccaaa tgatagaaca gagcgaggga gaaaataaag
    13141 aacaactaag taaatccctc cattatgtta tgatgcaact ggtctctcat tatgctccaa
    13201 aatcacccat atcggaagct tataggactt tgagaactaa tattctatca aaaaaacctg
    13261 aaggtagcac ctctatcctc ataacatcac ctggacctaa agaaggtaaa tctaccacca
    13321 tatccaatct tgccataacc ttttctcaga tgaatgctaa agtgatttta gttgatcttg
    13381 atatgcgacg tcctatgctt cataataaat ttgctctgga aaaagaaaat ggaatcagcg
    13441 attatctaat tgacccagat gttccgttaa aacagatcat taaagcttca ggtattccta
    13501 atctggatat cattaccagt ggttttatac cacctaatcc ttctgagcta atttcatctc
    13561 caaggactga taaactaatt gaagaattaa aatctcttta tgattatata ctttttgata
    13621 ctccacctgt tattgccgtg actgatgcct tgattttgac taaaaaagtt gatcttactt
    13681 tcttcgttgt gcgttgtgga accacagaaa aaggtatagt caaacgtgca agggatatga
    13741 tggagaatgt taatgctaac atagatggca ttatcgtaaa tggtgtttat gtccaaaaat
    13801 actatagtaa atctaactac tactattact actattatta ttactattat tatggtgaag
    13861 aaaaacctaa aaaacaaaag aaggctaatc tatccttcat acgcaaaaat aaatctattc
    13921 cttgaaatat taggttcttt acctaataac tttcatcaga ttgagacact gctctgcagt
    13981 gtctcacttt ttgacactat caaatatact ttgacaaaaa ataaagattc gaaaaaatgg
    14041 atttacttaa atgataagat aaacaaaaat aatcttatct ataaagtcgc agaatacttg
    14101 tataatcaat ataaacctga ttggggaatt gagattcaat tgataaaaag gatacctata
    14161 gcagctgggt taggtggagg cagcagcaat gcggcaacca ccctgttagc tcttaactct
    14221 ttatgggagc tcggattaag tattgacgag ctaaattcaa ttgctgcttc atttggaagt
    14281 gatattccct attttctcta tggtggtact gcacttgcaa ctaatcgtgg cgagattatt
    14341 caacctatag aggatttcaa gttggacaat attttattgg ttaaccccaa tataatgatt
    14401 tccagttctg aagcttatcg catagcttct attcctgctg aaaatgaaag aagacaatgt
    14461 ataatttcgc aggatgtaaa ttggtttttc aaccgacttg aagttcaggt gcgtatggat
    14521 tatcctttag ttaacgagat tttggacgat ctcataaaat ttggtgcgag cactgctctt
    14581 atgagtggta gtggtcccac ttgttttggc gtttttgaag aaagacttaa aatgcaaaaa
    14641 tgtgaaggat actttaaaga aaaaggatac tggacaaaag tcgtaagaac aatatctaag
    14701 gaagaatatc aaaatgtatt ccaagcttaa actaattagc ggtcacgcaa atagaccttt
    14761 agcggaagag gtggctaatt atttaggtat acctttatcc gatatagacc tttttaagtt
    14821 ctctaatgag gaatccttcg ttaaaatcaa tgaaagtgtt cgtggcgcag atgtattcat
    14881 aatccagcct acttgcagac cggtaaatga taatttaatt gaccttttaa taatgataga
    14941 tgctctcaaa cgagcttcgg ctcaacgtat caattgcgtt attccttatt atgcttatgc
    15001 tcgttcagac aaaaaagatc agccccgggt gccgataact gccaaattag tggctgattt
    15061 attgactgta actggtgcaa atagggttgt tgcagttgat ttgcatgccg agcaaattca
    15121 agggtttttt agtatacccg ttgaccattt atatactatc cccatttttg ccagatattt
    15181 taatgagatg aatctggata atccggttgt aatatctcct gattctggtg gtgctaatag
    15241 agctcgtgct ctggcaaaaa ggttaaattg cggtttagcc ataggagata aaagacgcca
    15301 ggctaatgat gataagacgg aattattaaa tattataggt gatgtagaag ataaaacagc
    15361 gattttatat gatgatataa tagatacagg aagcagtttg ataaagattg caaatgtgct
    15421 gagtacgaaa aatgttaaaa atatttatgc agcttgcaca catggagttc tttcctgttc
    15481 tgcagttcag aatataaaca attctaatat tgagaaacta ttcattacta ataccattcc
    15541 tctttctgaa gaagctgctg cttgtcccaa aattattcag ttatccatag ctgagatgct
    15601 ggctatggca ataaaaaaaa tacatacaga agaatctctt agtgttctat ttacttagga
    15661 tttgagattg gaggtagaaa agtgatattt accattgaag cccaaaaaag aactaacact
    15721 aaacatagtg aattgactgc tctacgagag caaggtctta ttccagcagt tatttacggt
    15781 aaagctttag aaagcacacc aattgcagta aaaagaaatg agtttctaaa atgctataag
    15841 aaaagttttc aggaactatc cttctatgaa attatcttag atggacaaaa ataccatact
    15901 atcttgaagg ataagcagat tcatcctgtt tccagagatt ttctacatct ggattttatg
    15961 ctggtagaag ctactgctat gatggaattt gaaatacctt tgcaatttgt gggtgagccc
    16021 ataggtgtta aaactggagg tttcctggaa accttgcaga gaaccgttaa gatttcctgc
    16081 aaagctgagg atgctcctga agaaataacc attgatatct ctggaatgca aattggtgat
    16141 tctcttcata ttaaagacct tcctcaaggt aattggcaat ataaggataa tccagataca
    16201 gcattattag tagttcttgc tcagagagtt gaggaagctg aagctccaaa agaagctgag
    16261 gaagttagta ctgaagaaaa aaccgagtaa tgaaactcgt tcttggtttg ggaaatatag
    16321 gtaaagatta tgaaggaacc agacataata tcggttttat gtgcctggat acctgggcta
    16381 aaaaacataa tctaactttt agatcaaacc agctatacta cttcattcaa tataaaaatg
    16441 cttctctcat caaacctaaa acctttatga atcgctcggg acttgctttg aaagaagcat
    16501 ctttaaagtg gaattgctct gaagctctag ttatttatga tgatatagaa ctccctttag
    16561 cctctcttag agtaagaact ggagggggtg atggtggtca taatggaata aaatctctgc
    16621 tggaaatatt cccgcctgaa aatcttaagc gtatccgtat aggtattgga aggagtgaag
    16681 aaaatccacg agattatgtc ctggataaat ttacccagga agaattaagc gctttaatgc
    16741 ctgctattga attagcctgt gatttcattg acatttatat tgataaagat ttttatgcag
    16801 tcttagatgc atatagcatc tggaaaaaaa ccttttccgt aaataatgtt cacggaaacg
    16861 atagtccaaa ggagaaatga atgaaaaact acgaactaat ggtgataatt tcaccaaaac
    16921 taaccgaaga agaagcaaat aagttgaatg aaagtatcct taatatggtt aaagaacagg
    16981 gaaatggaga aatcattaaa actgacccct ggggtttacg aaaacttgcc tatcccatta
    17041 acaagatgca agaagcttat tatttcgtta attacttctc tatggaaccc agtgcggtaa
    17101 aacccattaa aaatcaactc aacattaatg agcatgtgct ccgtcatatg tttattgtta
    17161 gagataaata ggaggaacaa tggcagactt aaggttaccg cgactgaata aggtatttat
    17221 tgctggaaga attacccagg aaccggagtt gagatatact cctaagggaa ctcctgttgt
    17281 aagatttact gttgcaacgg atcgtaggta caaagatgaa atggaccagt ggcaatccat
    17341 gcctatgtac attgatgtca tagcctggag caatcaagct gaaaatattt gtaaaattgc
    17401 gcataaggga actgctgttt tggtagaagg aaagattgat acaagaagct atgtggattc
    17461 aaataacaat aatcgtaaag tatttgaaat cattgcagat catatccaat ctttagaatg
    17521 gactcccaaa gcagagggtg aaggtgtaga agaagaagta ccccttcccc aagaagaacc
    17581 taactatgtc gcagaacccg ttacggacga cgatgtccca ttttaatact ggagttttaa
    17641 tatgaatact atggatagaa aaaagaaacc tgctcacaaa aaatactgcc gcttctgtgc
    17701 gaataaagac ctcgttattg attataaaaa tgtggaactg ctaagtcagt ttatctctga
    17761 tgtaggcaag attgaatctt ctcgtttaac tggcacctgt gctaaacacc agagaaaact
    17821 gagtaaagag attaaacgag ctcgtcaaat ggcattattg ccatatgtta ttgaatagtg
    17881 ccgtttatcg ctttactgtt aggtgcttta gccacattaa atccttttat cggattatta
    17941 gtgatgatga tttatagcca tagcttcttt cgtttgttga ccggaaaacc agttaatcgt
    18001 gccctaatgt ttctcgtttt cccagtaatt gtggctattt tcacctttaa taatccctca
    18061 actcttattt atcccgttga tgccatattt ggtgcagggt tagttgtaat tctatatctg
    18121 tatgctctta tcaaacgagg taatcctgtt actgcattaa tcaatgctgc tttattgatt
    18181 accgtttatg gagtgattag atattatctc tttggaagtt ttttactcaa cacattggaa
    18241 cagtcttttg cggaaatgga aaaaattatg cctcaagcta tgcaaaatgg gaatataact
    18301 cagatgaaac ctatgctgca aatcctgtta cctgcaagct ggactgtatc tcaagtttta
    18361 gccttaagtg cgggacatct aatatttctc catttaggcg gaactcattc cgagctaaga
    18421 gattttaaac ttccagcgta ttacaatcta tttattatag ctgtcctgcc cctatatttt
    18481 ttccctcagt tgcatagtgt ttttattaat gtattattag ccttatgtgt gttacctatg
    18541 gtagaaggaa ttggcgtgat tattaattta atctcaaagc gaaaaccgaa tattctgatt
    18601 aatatcctgg ttactatctt agtactttta aatttaccca taatggctct aataggtttt
    18661 gccgatatct ggcttgattt tagaaaatta aatattaaag gaaatttatc atgaaagtta
    18721 ttcttttaaa ccccatagaa aagttaggaa acaaggggga tgtggttaat gttaaacgag
    18781 gctatgctcg taattacctt atcccaagaa aactggctat ttatgctacc ccccaaaatc
    18841 taaaaaactg gaattctatc caaactaaag ctgctgaaga agaagaaaaa atgattgatg
    18901 caatgaaaaa acttgacgag cagctaagaa atatgactct gcattttgaa cgtaaaacgg
    18961 acgaacaaaa taatctttat ggttcggttt cggaaatgga tatctgtaat gaactcgcta
    19021 aacatggaat taatattaaa tgtgcaatgg tcaaaatgga cagacatatt aagtccctgg
    19081 gagaaagttt ggttaaaatt catctataca aagatatatt ttcagaattg aaggtcgtgg
    19141 tcgataaaga aggtagtggt cagaagaata tagaacccaa gattgataat tccgctccgg
    19201 aaacttctga tgaagcagaa agtgaaataa ccacagatta atatatacgg ggtgttacct
    19261 atgaaaaaat atatgtatca aatctcacgc atcttagcct ttgttgctat gctcgtggct
    19321 gtttctatgt taagtattgg taatccgaaa aatataccct ttttcatcct gctctttgct
    19381 gctatttttg ccttagttta ctatttaacc aagcgttcag aggaaaacca acgcttaggg
    19441 aaagctggaa caggaaccaa acctatcact caattattaa ccgtagtcct tttcgttgct
    19501 ctcatctcca ttttaggttt attaaggcac ggaatacttg gttttatcgg atggttctta
    19561 gttattggtg gcatcttttc ccttatctat cttagtgttc gtaagcatca acgccatttt
    19621 gaaattatca cttccactcc taagaacaca aaagttacag gcataattct ggctattata
    19681 tctattctct tgcctgttat agtcatttct tttactaaca ttgtccctat agctaaagca
    19741 tctctcgcct tatctatcgt tctttctctt atcggcactc tggcatttgt ggttctgatt
    19801 tgtctgggat tatatttaat aaatatcatt ggaaataaga cctccaatct tattctcggt
    19861 tatctggcta taattctggc agcattatta cctggtataa ttttactatt tacttctgcc
    19921 actactacgg cttgtgctgt aatttatatt attgccatta ttacagcgct gctggcatat
    19981 tttgccttta atcagttggt cacaaataaa tagctctaag ggaactttgt ttagacccga
    20041 gaaggaatac tatggctttt acctctcttt ctaatatcaa gaaagaggta attgatagaa
    20101 tagcaggaga aaaatataaa tcctttgtta acctgtatca gcactggaaa gatattgtgg
    20161 gacctctttt agctgccagg tctcatcctt ttcatttaaa ggattctatc ttatatgtag
    20221 ctgtagaaaa taactcctgg ctacaggaac ttgtcctacg taaaatggat attttaaaac
    20281 aacttaatga taaaaccgga gaagagatta aagatattat atttttaatc aagacttaat
    20341 actatgaaac ctattcaaat cgctgcttct gtgctatcct gtgattttgc tcatttagct
    20401 aaggaactgt ctcgtgccag tgatgctaat atgtttcatc tggatattat ggatggacat
    20461 tatgttccta atctaacttt tggacaacct attgtattaa aaatccgtca attaaccaaa
    20521 attccattgg acgctcatct gatggttact aatcccgaaa attatgttgc accttttgcc
    20581 agtataggag ttaactggtt cagtttccat caagaatgtg tatatcactc tcatagggta
    20641 gtgcaacaaa ttaaaagtct gggaatgaaa gctggaatag cactcaatcc tgccacacct
    20701 ctttctacct tagattgtat tttgcccgat ttagattttg ttcttttgat gagtgttaac
    20761 ccagggtttt ccggacagga atttattcct tctgttctgg aaaaaatcat tcagctaaaa
    20821 gatattataa ccaagaattc tttgaacacc ttaattgagg tggatggcgg aatcaatgaa
    20881 actaatgcct ccttagtgat tgctgctgga gcagatatcc tggtttctgc ttcctatata
    20941 tttggcagca ccgattattc tgccgctatt cgttccttga gaggtagtta attaatctaa
    21001 ttttttaaat tctttcccca tccataatag ctggttcaga tattaacccg gagggaaaga
    21061 ttattataaa ggtaaattat gtttgattca ttaactgata gattagataa agttttccgt
    21121 aacctaagag gcaaaggata tctcaccgag caaaacataa aagatgcttt gcgggaaatt
    21181 cgtattgccc ttctggaagc tgatgtaaac tatcgcattg tcaaaaactt catctctgaa
    21241 gtggaaaaaa gagctatcgg agaagaagtg atgaaatctc ttactcccgg acagcaagtt
    21301 gtaaagatag tttatgacca gctggtagct ttaatggaca cggagggatt tgaacttaaa
    21361 gtctacaaca ataaacttac taaagtgatg ctggtaggtt tgcagggctc aggtaaaacc
    21421 actgcttgtg ccaaattagc cctttattat cggaaaaagg gaattaaacc tatgatggtg
    21481 gcttgtgatg tttatcgtcc tgccgctatt gaacaactca aaattctcgg taaacagatt
    21541 gatattcctg tcgtttcgga agataccaaa gatgtatata aaattgccga aaaagcttta
    21601 aatctcgctt cacaacaatt gattaatctt ctcatttttg atactgcagg tcgtttgcag
    21661 attgatgaac ctatgatgga tgaactggtt cgtttgaaag cccagattaa acctgattat
    21721 atcctttttg tcgcagatgc aactacagga caggaagcag ttaatgtcgc taaagaattt
    21781 tacgataagc tcacttttga tgccgttatc cttaccaaac tggatggaga tgcacgcggt
    21841 ggcgcagctt tatctattaa agcagtgacg gatagaccta tcgcctttgt cggaataggt
    21901 gaaaaaatta gtgacctgga agtattctat cctgatagaa tggcatcccg aattctcggt
    21961 atgggtgatg ttctcactct gattgagaaa gcggaacagg ctattgacga aaaggaaagt
    22021 gaaaaacttg ccaaaaagat gttgaaaaac cagttcactt taaatgattt cctgaaacag
    22081 ttgcaaaata tcaaaaagat gggttccctg gaatctatta tccgtatgat tccgggtcta
    22141 ggacacaaac tccctgccga tttaaagatt gatgataaag catttaaaca tattgaagct
    22201 atcatccaat ctatgaccga aaaagaaaga gaacatcccg aaatcattaa tggcagtcgc
    22261 cggctccgta ttgctaaagg aagtggtcaa tcagttacgg aggttaatcg cttgcttcgc
    22321 caatttgatg aaatgaagaa gatgatgaaa cgcttttcat ccccccaggg aaaaagagac
    22381 ttagaaaata tgatgaaaag gtcaggataa gtgttagtcc taccactcta ttttctttat
    22441 taaataaata tcgctcaagc ttagacaata tattatccac caataagtta acccttagag
    22501 actcaatact tattatcttt attatagaat accactgaac tcatatcaaa tattatttat
    22561 ttttattatt atttgttaaa gttttctata ttctccctta gctcttcctt ggctacctct
    22621 tttctccaaa ctaaggcggg aaacaagaaa gaggtaagaa tgtgataaga aagagcagtg
    22681 aaaaagttaa tatattagga tatttataat aattaattgc gggttttcat atagctgatg
    22741 atataattga tgattattcc tactctacta tctgcctaat aatccagctt gctgccatca
    22801 tccccattat agctggcata tagcttatcg ttcccacttt accacgctct ctgcctctat
    22861 gaatgaggtc ttcatctgga cttgaaatat ccttaaaaat cttttttgga agttcagaag
    22921 aatagacaca aggaaaatta caatccactc cccatctttt taaatgcttt ctcactcttc
    22981 ttgccagagg acaattaacg gtctcattta aaggtgcaag gtgaatctct tccggattaa
    23041 gtctgtttcc tgagcccatc acagatataa ttttaaaatt atttttcaca gcatattcca
    23101 gaagaccaca tttaggacct aaactatcaa tggcatcaag ataaaaatcc tgctccttaa
    23161 gaatctcttc cctattggta gaatccagaa aaacaggata ggttatgact tctgctgcgg
    23221 gattgatgtc ctctatatgc tttttcatta cctcagtctt gggttctcct aaagtatgat
    23281 tcaaagcatg caattgccta ttaaaattgc tctctcctac cacatcaaaa tctaccaaag
    23341 tgaaatgtcc tattcctgca cgagctaatg cttccacagc ataggaaccc accccaccta
    23401 aacctattac tgccactcgg gaattattta accttgccat tgcttctgac ccaactaaaa
    23461 gagctgttct actgaatctt aattcactca taattttccc tgcgtcaacg ctataaagtt
    23521 ttcatactga gtggtaagta tttcttcagc gtctaccctg cattgctttt gcacctgctc
    23581 aaatacatcc actaatctta atagtgggtt atgctctcct ggcaggacat aatccttggt
    23641 tatatcggtt tccaatagat atcttttatc ttttaccatc tcctgcaaga ggttatactt
    23701 atctggtcgc aaaattcttt cactgatagt aaaatagcta ttcagacggg aaagtatgcg
    23761 ataaccttcc agacttccag cataaccatg caccagatat accaaaggat aaagttttag
    23821 caattcataa gcctgttgct gataaccaac tatatgcagt actacgggaa gttgatattg
    23881 atttgccaac tccaattgct gggaaagaag ttgtttttgc ctttctatat ccttgccata
    23941 acggtccaga cctatctctc ctacagccca gatacatttt tctgcacaga gttcttctat
    24001 atcttttagc tctaaatcac attctgcata atgaggatga atgccagcac taaaaagaac
    24061 tcctggcaat gaatattgac gatgaaaggc aatctcctct ttagataaag cagaagagag
    24121 aaaatgagtt attcctgctt catttgcctc tttcagcaaa ctttctacag gcattatctg
    24181 attcaagctt gccagatgac aatgtccatc aactaaattc attttttcag ctccggaagt
    24241 atctttaaaa tttcatttgc cactctaaaa cctgctttcc catccagttc atcaaagaaa
    24301 atagcacttg cttctttcct ttcttcttgc aattcttccg gatgagctaa acagtgttct
    24361 atggcaggtt ccagctcttc aaaggtagct atttgccatg tgcatctggc taaaatctcc
    24421 tctatttcgg gtacagttct ctcgcttgta ggcactttcc aggtgataat cggcttatct
    24481 aaagcacaac actcagccag aatagaggat gtatcaccaa tacagacatc gctttccgca
    24541 atatatttta agggactctc ctcttctata agtttcactc cttccgtgtt cttgatgatt
    24601 ttaacatatt ttctggacat ccacggatgc aaagtaacat aaatatcata ttttccagtt
    24661 agtaatccca gcttatctgc ccagagatga atggcactca ttccactttt atcataagtg
    24721 gcagtaaaaa ctattattgg cttattagat ggttttaatt ttttaaaatt ataataaggg
    24781 tctaattttg g
//
LOCUS       DAORZF010000025        24710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_1689_length_24710_cov_338.695153, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000025 DAORZF010000000
VERSION     DAORZF010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 24710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24710
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1689_length_24710_cov_338.695153"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            866..4918
                     /locus_tag="PK015_04315"
     CDS             866..4918
                     /locus_tag="PK015_04315"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOL33725.1"
                     /translation="MKKAFLLIALMLFATFIFATIEEYYSFNYYTGTYTPISGTPVPN
                     ISQSKISDIISLPFPFPYGSYLYREVQVSYGGCIGLGHSKSTSIPFINLTYYAPLIAP
                     FWDSFYTNNNSDFQYLICDEAIDRHIVIQYSSMYWNDCIVNFQAYLYESGKIEFHYGP
                     STGIPYYPRGSIGINMLPGGPGWFYNVIPTSEPIITTNYDNGNVSHFPQCNTVYEFNP
                     VIAHANDLTTFLIESFTTPAVGQSSDFIITVYNRGANSQNNYEVKLFLNDDIEIGSVK
                     GSLINTLETLEFVISWTPAITGHYSLYAKVLLNNDEYIENNQSPPLEVEVYPEGTVFT
                     TVGKMQRYGQMPLLFTYQRSIFECIYPADKIHCWGLITGIQFYYNSNRTISNNEIRVW
                     LGETTQINLSNNWIPSTQLTKVFDGFLSFSAGQNIFYINFDIPYTYAGENLIVMMERS
                     NLFPDPYENSSWQFYFEVQESNIENCARIIEFSDLQHNASNPPDVSPVSIFPVTTFKF
                     MTTGFGPVLGKVHDSNNMPIVSAEVSFSGSSFIATTNAAGFFQFPFLLEGTHQIKVSK
                     YGYYDNIQDITIGAHQFLITDFTLLPLALVSIRGMVVGNDQSDGGLAGATVNFSGYAN
                     YTTLTDMNGQFTINNVYSDHIYDYVVQKNGYRNATGEVNVGNTDLFLSALVLNVIAFT
                     PQQVQAIQASDSSYVSISWLSPIQYLPELEESFEGDIFPPPEWLQFINNAVQINNYGV
                     STTWGKVGMVHSYPPIIPHSGSYQVGIVPSQSDQNEWLITPQFTCPENAILTFWSYVF
                     LGDQGEGGCNLMISTNNGNGWLSIWNAKSKYGGLNNYEKPITVNLSNYSGRQIRLAWI
                     YSSPQEYGWFLDDITVSNSSIVLKFPLESIKTSDINSSTIIKPDFPESLSNAKDNERI
                     LIGYQIWRLPYGTENNPENWTLLTETPIPELSYQDNSWNSIPDDIYRWAVRSVYTNNI
                     QSNPVFSNALSHGFQAGVISGRVISGTLYYSFPVFKATIFCGSNSTTTDVAGYYNLIV
                     PSGTYNVSASAPMCLNQTITDVNVNPNQITEVNFILPYGSYSSFDNFESYEDFALIFE
                     PSAECYILRTIDLDHSPTCTLEGVNWPNAGLPMAFMVFNPFATIPPLSGALPFSGYKE
                     AACFGSVNNPNNDWLIAYSPSNVGFQFWARSYSPDNLARFKVGISWTDNNPSNFTIIS
                     GENYIEAPYVWTNYSYNTEAPYYGIQCVSENGAILFVDDLQKGFVTIDDPIATPSASE
                     ILGNYPNPFNPETTIRFSVKETSSVILEIYNQKGQYINTLVNETKTPGNYSVIWNGKD
                     YNNRAVSSGIYICRLKVGKNISTRKMLLMK"
     gene            5222..5299
                     /locus_tag="PK015_04320"
     tRNA            5222..5299
                     /locus_tag="PK015_04320"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5257..5259,aa:Asp,seq:gtc)
     gene            5353..6582
                     /locus_tag="PK015_04325"
     CDS             5353..6582
                     /locus_tag="PK015_04325"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HOL33726.1"
                     /translation="MTRFLFHFFNILLEICCLLLSPLILIFIGIRRYWGALNFKATDS
                     EKGIMIHSASVGEVNAVRPLVKALQEKYPRLKIVMTCTTLSGLEQAKTLHIPAYLSVL
                     DLPWLRKKQLQSINPQIIIIVETEIWPNLLYQAKKQGIKIAFVNARLSEKSFNRFKKW
                     KKLLHFLEEPIKIIMAQTQEDTVRFRNLFTVPVLYVGNLKFALQLPQYDELELRMKYG
                     YKPEDFIICFGSSRPGEEALLKNILPELKLRIPELKLVIAIRHPYRIGEVRDLFPEAA
                     IYSELQENVNDSGDVLLIDTIGHLNEFYAICDLAIVGGSFFDFGGHNPLEPAFYGKPI
                     IMGPYHSSCAGSVQELFSFNAILISDSQNLKRDIIYLYQNPEKRKEMGENARACIEQN
                     KNALENHLRGLEPWITA"
     gene            6567..7565
                     /gene="amrS"
                     /locus_tag="PK015_04330"
     CDS             6567..7565
                     /gene="amrS"
                     /locus_tag="PK015_04330"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOL33727.1"
                     /translation="MDNSIEREAMFYEKLDNQQVRCLLCPHACLLKVGETGTCRGRKN
                     ITGKLIAINYGRTMGISLDPIEKKPLYHFRPGSQIVSLGPNSCNLSCFFCQNYDSSQM
                     PCPTAYINPEQLYNLVLEHSYQGYKQVAFTYTEPFTWYEYIYDFAKLARDTDIVMVTN
                     GYINPAPLKELMPYIRAMNIDLKSIRNEFYVKHCNGGLETVKQTIKNAFNAGIHIELT
                     NLLIPTLNDAEEDIQALIDFVSSISVDIPLHFSAYYPAYKSNIPPTSSRIVLKACQMA
                     KEKLSYVYAGNIWSNEFRETHCPQCDNLVISAHRRIVGIDDKGQCRNCSYRIYGVF"
     gene            7565..7930
                     /locus_tag="PK015_04335"
     CDS             7565..7930
                     /locus_tag="PK015_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HOL33728.1"
                     /translation="MFKWLKSHLSWADFILILVVIIAIIISGILVVKKEDERNVYIYK
                     NNQLIGVYSLEQDRIIRIDEHNTVEIKNSKVRMLYADCPNHNCVRQGAGDILPIICLP
                     NKVVVEIHSKNAKRPLIVQ"
     gene            7938..9908
                     /locus_tag="PK015_04340"
     CDS             7938..9908
                     /locus_tag="PK015_04340"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33729.1"
                     /translation="MLKFAFYISHHGFGHTTRMAALAKEFNEFDIFVYIRSAKPDYLF
                     QDLNPHLYEKEDTICDVGVKHKANLEPDKAATRLALLQLMGKRQEIIDREVDFLRKEK
                     VDLIITDIPWLPVEAGTYAEIPVFAISNFDWLFIYDKLFGKQTDLKPVLNTIYGLYQR
                     VDYAFRLPLSSTKSMGSFRKIEKTGLLAAYKPPNPELKKALGIDSKIPVLTCSFGGEG
                     EMNLNWKNMCSAFPGTVISTRELKEIPNYIQIPPDFDFSSLISISDILLTKPGYGSFA
                     EAIQSGTFLIYYPRKDYPEEEVLIKGISSYPQKIQLPELNLSVSEWKNLFHTALTFSG
                     HRKIIPNRNKQVASLILQRYIELHYSQKKLNSIFDIGSNNLNYALCEAGKSVPVHTAQ
                     IKTGIGREYKIVKGTVRIKREAIKRFKSLVSNFMAYDQNIPSSKFVIATGIHRQSPQL
                     QQLSEWFNKKWKAKYRLLKEGEEAELAYLAAKDLIPDKQSAIIIDIGGFSTQFIYSEP
                     DSNIDRMSIPIGLLTIRKTVQEGKKLNNVLDKIAKSIPFWKADMIICVGLTATFLAKI
                     VKRSRYYRPDELHGCRISLKELLTLKDILESGKNEEIANYAMEPESLDILYYSIQYYI
                     FLLDRLQSSGFLVCYYGIATGYNQKLNKINKR"
     gene            9913..>11043
                     /gene="miaB"
                     /locus_tag="PK015_04345"
     CDS             9913..>11043
                     /gene="miaB"
                     /locus_tag="PK015_04345"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOL33730.1"
                     /translation="MKFYIETYGCQMNIADSELVSSILMDAGHQLVSNIDEAELIIFN
                     TCSVREHAENRVLGRIATEKYRKTINPELKIAVIGCMAQRMGKRLIENYGIDYIVGVD
                     KYLELPELIQQEKGVKTETDNLQTYTGIMPLHQSQTCAYVTIMRGCNNFCSYCIVPYV
                     RGRERSLPAELILEEVKRCGEMGLKDITLLGQNVNSYSYQDINFPKLLRQLNDIDSIY
                     RIRFITSHPKDLTDELIEVMAESSKICEHIHLPLQSGDNDILAAMNRNYTIEHYLNIV
                     DKLRKAIPDMAITTDIMTGFPGESETAFNHTLQAMQEIQFDYAFCFKYSSRENTKAAY
                     LPDQVPEEIRLERLKRMVNLQRQISLKKYEAKIGSEVEVYVES"
     assembly_gap    11044..11143
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11162..12037
                     /locus_tag="PK015_04350"
     CDS             11162..12037
                     /locus_tag="PK015_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOL33731.1"
                     /translation="MKSMTGYGKAKIQKNDMDCEIEIKSINGRFLDMRIYLPREISFF
                     EYPIRQHIGKVLSRGTVEIRVTFADRREPALKLNEVKLVKYYELVKQAQALLDIDEDI
                     SLEYLLNEPGIIESINNLDEDEQLAELLNQTLDKAIEALELSLRAEASNIKETLRHSS
                     DLMLKTLDNIEKEIQPFKEELYSILKHRVTELLSNFNAEMLEQRLVQELAIYIDKYDI
                     QEEISRLKCHINTLLATLEKEEDTGKTLNFILQEMQREANTLGSKFSTARTFTYVLTL
                     KEEIEKCREIVQNVA"
     gene            12027..12464
                     /locus_tag="PK015_04355"
     CDS             12027..12464
                     /locus_tag="PK015_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33732.1"
                     /translation="MSPEPLLTWRSWPFIERPFTSVLLILFLLFLAYFLWNVTVEEWH
                     QPWYYYLGMLLVIGTLLPYFIPTTYYLYENEIVIMYLFIKITRKYSDFGCFYCDKRGV
                     MLSTFKMPRRLDPFRGQSLRFSKTQTEKEQLLEILREKIGKQY"
     gene            12472..14766
                     /locus_tag="PK015_04360"
     CDS             12472..14766
                     /locus_tag="PK015_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - exoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HOL33733.1"
                     /translation="MKNTNLSDRIEQTFIENNNTPLSYNELVNELHLVRREKSLLAET
                     LQAMVAEGILTKKNRKFRLAVSPDQLKKETYEPVSHPRLLEGIFDATPLARDLSYAFV
                     RTEQGDFYVNAEDTLNAYHNDIVAIEPHFRKGKPDYAIVRKIIKRANETMTGDIRESG
                     SRTFFISINPKIHNWIEVSDLNGAKEGDKVVLEVTNWGNPIMGRMPVGKVIEILGPSG
                     DPEVELLAVIRQYNLPLQFPDTVISEMEAIEESISDKELAHRLDLRNILTFTIDPASA
                     KDFDDAISLERINQGWRLYVHIADVAHYVKPESATFSEAVKRGNSFYFPKKVIPMLPE
                     KLSNKVCSLRPNEDKLAMSVITDFNLQGKIMRQRLEESVIRSDYRLNYDQVDELFSGK
                     NTDFPSDLSETLLSARKLSSLLTAKRLKEGYIFFDLPELEYEYDEEGFLKRFTLAEET
                     ESHKLIENFMLVANECIATKLSKLAPATIYRIHEDPDWEKIERLIELLSYYGISFYER
                     ENLNASIQYLLHSLPTPEHHKVFEPIILRSMKKAKYSTEHIRHFGLGMETYTHFTSPI
                     RRLCDLVIHHLSKTYILKTSKIQFDYSQLKHFASVASEQELQADQSERDIEHYFSMTY
                     MKKFIGESFSGIVMSAKNDRLIIRLDQIPINAVLMVTEFPPGNWIFLDRQMRFINEDN
                     RDYFQLMDKVLVNIMEVSDDIYLELKPGKESHRHFQFQLSNKNSFNNDNRIRKNQRGK
                     KRFDVDYKFRNKNYGNTNKRYRGK"
     gene            14766..15566
                     /gene="murI"
                     /locus_tag="PK015_04365"
     CDS             14766..15566
                     /gene="murI"
                     /locus_tag="PK015_04365"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOL33734.1"
                     /translation="MKQPIGIFDSGVGGLTVYKAIKRAFPAENIIYFGDTARVPYGPK
                     SENTIIEYSIQNARFLLQKDIKILVVACNTSSAVALQELEKLTDIPIIGVIKPGAKLA
                     ADSTHNNHIGIIGTEGTIRSEAYNKAIKTILPEAEIFSCACPLFVPLVEEGWLNHPVS
                     RMVAEEYLNFFKDKEIDTLVLGCTHYPLLKSLIDEIMEHKVKLIDSADAIALHLKTLL
                     PVESSTEPVMDRFYVSDNEAKFSQIAEYILGVQPTPIKRVRLYESWFE"
     gene            15585..16409
                     /locus_tag="PK015_04370"
     CDS             15585..16409
                     /locus_tag="PK015_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HOL33735.1"
                     /translation="MEPLILTAAITGAETCRKDQPNLPITPEEQAAEALACFEAGARI
                     IHLHVRDDEGNPTQSLERFEAAITAIKSAVPEIIVQISTGGAVGEDFEKRLAPLCLKP
                     EMATLNAGSLNFGNDVFINRPPDIVRLAEAFKEYQVVPEVEVYESGMIDYVAKLIKKG
                     VITHSPLHIQFVLGVPGGMNGSPKNVVYMAYHLKELIPSATWAVAGIGQYHIPASLTA
                     MVMGGHIRVGFEDNIYYHKGVLAESNAQLVARMARIAAEIGRPLATPAQAREILGL"
     gene            16420..18666
                     /locus_tag="PK015_04375"
     CDS             16420..18666
                     /locus_tag="PK015_04375"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HOL33736.1"
                     /translation="MELSENALIVLERRYFRKNEAGQTIENWEGMINRVASNIAQGSK
                     DKEKLYFELLDSGMFLPNSPTLMNAGSDLQQLSACFVLPIEDSMESIFETLKNAALIH
                     KSGGGTGFSFNHLREANAPVRSTNGVSSGPISFLKVYNAATDAVKQGGTRRGANMAIL
                     NVDHPQILEFIQCKADPKELTNFNISVGVSEAYMQAVLNDNDYDLISPHSGKVIRRLK
                     ARDVFNLIVEMAHRNGEPGIIFLDKINAANPTPKLGRIESTNPCGEQPLLPYEACNLG
                     SINLAKFAKDGSIDWDKLQSVIRLSVDFLDDVIDCSRFPLPQIDALVKANRKIGLGVM
                     GWADLLFQLEIPYTSEEAISLAKELMEFIDFEAKQRSMELAKEKGSFPNFPDSIYDTL
                     SLKREPIKKDWDKLLSDIKQNGIRNATVTTIAPTGTISMILDTSSGIEPLFSLVYVKN
                     VMDGEKLIYANKYFQTALEREGLISPELLSQVSQTGSIKDIKEIPERFKQIYQTALDI
                     NPEWHIRMQAAFQKYTDNAVSKTINFPHSATIEDISKAYQLAWELGCKGITVYRDGSR
                     EDQVLSSGIKMTVSEGKVAPRSRPDVTYGLTQRLETGCGHMYVTINSDEFGACEVFIQ
                     MGKVGGCASAQLEAIARLSSLALRSNVKLESIIRQLKGIRCPSPMWHNGTLITSCGDA
                     VGQALENFLKIHNNLQVESFHMETETDSHIEEPGAKSAIYVCPDCGSTVQHLEGCLKC
                     PVCGWSKC"
     gene            18688..19491
                     /locus_tag="PK015_04380"
     CDS             18688..19491
                     /locus_tag="PK015_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33737.1"
                     /translation="MRCKHCDARLAPHDFWCVNCGKQTPIVVHELASWGSFKRTFKQY
                     WQFKGANIPAAAFAILVGVIPIIAFLVLLNAFGILDFQAQETAGKLLLNLFITGIGLS
                     LLTPLLLIAFKPVCEIKDYTLTLKDLLKGFKAYPRYFFFSLITALYFILIYIICFGLP
                     NFASDPILHLVWLVLVGYYIGVSLPVPILMERKKVNAWKAIRMAYKYFKVVRWQLFWL
                     GLILLVLNLAATALALVFLLITLPLSWFAIRDYIDLLLEYEIIREYKTK"
     gene            19503..20537
                     /locus_tag="PK015_04385"
     CDS             19503..20537
                     /locus_tag="PK015_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOL33738.1"
                     /translation="MTKKEIISLILLIVVVGYGAWTWINRSYSETKRDLDLLDTVVTI
                     SATSKSKDIGTQIDSVFAYIRTFEDIFDDYNPQSWVYKVNNAQGKRYPMNPDAYELLC
                     VADSLYQLTNGAFDITIKPLYDLWGFSKINEYGTDTLSLAPPDSMLIQETLKNIGFNR
                     IRYNKQYIEMPQGMQITFGALAKGYALDKARNYMENHKFISGIIDCTSSMTLFGQKRS
                     QIVSIKHPRANAGMQTIGNFKIKNGSLGTSGDYQLYFEYNGQRYHHILNPYTGYPVEH
                     VYSVTVINPSATWADGLSTALFLLPPDQAIEALKYYPDSNAVIFYQENDQIVSLKTLG
                     MKALDWRDEQ"
     gene            20519..21310
                     /gene="folE2"
                     /locus_tag="PK015_04390"
     CDS             20519..21310
                     /gene="folE2"
                     /locus_tag="PK015_04390"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HOL33739.1"
                     /translation="MARRTMSIPDIQSQDDFRQIAIEKVGVKGLSYPIVVDDRENGTQ
                     DTVGNINLYVDLHPSRRGIHMSRFIEVLNRYHHDSIIGNLESLLQDLKYIMKAEAAYI
                     DIYFPYFIKKLAPISKIPSLMNYQCFFNASFNNAFQFWIGTTVPVTTLCPCSRAISTN
                     GAHNQRALVTIKVRYTEFVWLEELIQIAEESASSPVYPLLKRRDEKYVTENAYANPKF
                     VEDIVREVTQKLEKDSRIVEFIIEVESMESIHNHNAYAMTHRLKH"
     gene            21328..23928
                     /locus_tag="PK015_04395"
     CDS             21328..23928
                     /locus_tag="PK015_04395"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOL33740.1"
                     /translation="MSESLVKPAVSGFLPNNLKYIYQPGNFEPVICLQLYIKIGSAWE
                     KEDEAGFSHFMEHLTFKSTVKFPFNQIAAYISKLGGSINAYTDFDCTCYYISLPSEFV
                     NEGLEVLAELAFRSTFTKEDVEVEKDIILEEMVQNTLDPETNFLQFVQDAAFTNYPLK
                     RPILGTKESIKKASYKELRSFYHKYYQPHNSFLVIAGEAEFNQLKSIIEELFGDWNSH
                     SKLIFPDFKQFLEPNFPPLPYLSKSSSQLFIAFVLPELSDKHPLSNAMLIAIRYLAIG
                     RSSRLFKRLVEGEKLASAVKVSSISGIMTGVSAIVVYPINEKSIPRIINIFQVEYQSI
                     LQGELDLNEIELIKKDIINTWRYGFECMEDLAEMIGDEEFIEGYENLYNYDEKIEPLQ
                     LEDIKKVITQYWQPSYLQIIQQTPHPGSLSLPEKLSYKKANITSPIPELKIPASLTHP
                     VPYKSIRPDFYSVKLANRLSLLYRYLPNRPISGFALATDICQLNEEKNQHGLNYLCSS
                     ALLHSSTNYNAEEILSLYRLYGVSLKVEHSLDTTIFRGKCFHKELLPALTLLEELIFH
                     PAFEEQYIKLLKTAAIDMLRRDKQVPYNEAFYHWFYLLFGKNSPYGRYSGNISDLKRH
                     NLESVVNWYKTNYRPEHFSLVVIGSLEPDKFFSITPQIFKEPAPSENWTGKNPEPPHP
                     IKKHKIIAHKSSPQAIIHLGGFAPAAKDRVDSTAFYLLAQIIGGDMDSRLFNLVREQK
                     GFAYQTGFEYNCAHSIGYWFAYAYCEPEVYKQCLELILQILQEVKENGVTETELLNAQ
                     NYLCGMNRFEAENASAQAMSISSLSALGYEPEYYFQREERIRSVDLKTISHLAKNWLT
                     KDNIWIYIYL"
     gene            23925..24704
                     /locus_tag="PK015_04400"
     CDS             23925..24704
                     /locus_tag="PK015_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOL33741.1"
                     /translation="MITLGIDIGSRNTKIVFYDTSNQSILFSAWSATEVNPLQSVHSL
                     LKQAQNSYGLDLPEEIACTGYGRKLWKGNSKVFSEITCHTTGVRYFIPEVRTIIDVGG
                     QDAKIISLTPEGKIKDFVMNDKCAAGTGRFLEMVASRLEVSLDELSELALQSKNPLTI
                     SSTCVVFAESEIIGMIAKNTDPADIAYAAHISIAKRIITQLSAVDFTPPIVFTGGVAQ
                     NKNLVQCLSELLNTTIITPPNPEITGALGAAILISQTEEYN"
BASE COUNT         8090 a         4079 c         4470 g         7971 t
ORIGIN      
        1 gtacccatta ttttaggtaa tgaataacct ttatctgtgg agctttcctc tttcactgct
       61 tatgttaatc ctcagaataa aataaatatc aagttagcta aaagaataac cccggtagtc
      121 ataccggggt ttttaataaa ggttaatcca atcgtaactt tagtgaccta atgaggcaag
      181 gatggatgaa aaaggatttt gtcttcatca aagttatcgt tatttgtgtc aatatggaga
      241 ggatattatc caatattaga aagaagctat tttctaacct tataagaagc aattatacat
      301 tcttaacatt ctcacatctg gtgaaacaac attacaaata ctatctgatg taaaaaatga
      361 gaggaattta gtcattgacc ctattaatga gttctaattt atcttatcac agtttttctt
      421 atggtatcta taggttttag aatatccgca gaattattaa tactgatgct aatcacgata
      481 ccattaatta ttttaggtaa tttcagctga ttaggaatca gctactatac caactgagca
      541 aacttagctt gaagaagaca tatctttaat gtttttataa agctatcaaa acatataaaa
      601 gtgctttcca gcaaggataa tttgaaaatt cagaataagt tttatttctt tacacgaaaa
      661 gaaaaaatat ttcttgacaa ttaaaagaat attttttaaa gaataaatta gctttagaat
      721 ttgctttttt aaattttgtt atgtagccag cttaactctg gaaaaagccc ggagaatagt
      781 tattgaacaa gaataataat taaagcgacg gtgattaaat aaaaagctgg tattcatttc
      841 aaatttacaa ggtaggtgaa aaattatgaa aaaggctttt ttacttatcg ccttgatgct
      901 ttttgctact tttatttttg ctacaattga agaatattat agcttcaatt attacactgg
      961 aacctatact cctatcagtg ggacacctgt tccaaatatt tctcaaagta agatatcgga
     1021 cattatatca ttgccatttc cttttcctta tggttcatat ctatatagag aggttcaagt
     1081 ttcttacggt ggctgtatag gattaggtca ttcaaaatca acaagtattc cctttattaa
     1141 tttgacatac tatgctcctt taattgctcc attttgggat tctttttata ctaataataa
     1201 tagtgatttt caatatctta tttgtgatga ggcaattgat cgtcatattg ttattcaata
     1261 ttcttctatg tactggaatg attgcatagt taatttccag gcatatcttt atgaaagtgg
     1321 caaaattgag tttcattacg gaccttctac gggaatacca tattatccaa gaggttctat
     1381 aggcataaat atgcttcctg gtggtcctgg atggttttat aatgtaatac caacttcgga
     1441 acctataatc actacaaact atgataatgg caatgtttca catttccctc aatgcaatac
     1501 agtttatgaa tttaatccgg ttattgctca tgcaaatgat ttaaccacct tcttaataga
     1561 aagtttcaca acccctgccg ttggccaaag ttctgatttt attattactg tatataatag
     1621 aggagccaat tctcaaaata actatgaagt taaacttttt ctcaatgatg atattgaaat
     1681 aggatctgta aaaggttctc ttattaatac tctggagacc ttagaatttg ttatatcctg
     1741 gacacctgct attaccggtc attattctct atatgcgaag gtcttattaa ataatgacga
     1801 atatatagaa aataatcaaa gtcctcctct tgaagtagaa gtttatcctg aaggtacagt
     1861 atttactact gtaggaaaaa tgcaaagata tggtcaaatg ccattattat ttacctacca
     1921 aaggtccata tttgaatgta tttaccctgc agataaaata cattgctggg gattaattac
     1981 aggaattcaa ttttactata attcaaatag gactatatcc aataatgaaa tcagagtatg
     2041 gttaggagaa actactcaaa ttaatttatc aaataattgg attccttcaa ctcaattaac
     2101 taaagtcttt gatgggtttt tatcattttc tgcaggacaa aatatatttt atattaactt
     2161 tgatattccc tatacttatg caggagaaaa tttaatagta atgatggaac gttccaatct
     2221 tttccctgac ccttatgaaa actcttcttg gcaattctat tttgaagtac aagaaagtaa
     2281 cattgagaac tgtgccagaa tcatagaatt ttctgattta cagcataatg catcaaatcc
     2341 ccctgatgtt tctccagtat ccattttccc tgtaactact ttcaaattta tgactactgg
     2401 cttcggtccc gttttaggga aagttcacga ttccaataat atgccaatag taagtgcgga
     2461 agtatctttt tctggttcca gttttattgc aactactaat gccgctggtt ttttccaatt
     2521 tccttttctt cttgaaggaa ctcatcagat aaaagtttcc aaatatggat attatgataa
     2581 tatccaagac attactatag gtgcacatca gtttttgatt actgatttta ctttgttacc
     2641 tttagcttta gtatcaataa gaggaatggt tgtgggaaat gaccaatcag atggaggatt
     2701 agctggggct acagttaatt ttagtggtta tgcaaactat actactctaa ctgatatgaa
     2761 tggacaattc accataaata atgtttattc tgaccatatc tatgattatg tggtgcagaa
     2821 gaacggttat agaaatgcaa caggagaagt aaatgttggt aataccgatt tattcctttc
     2881 ggccttagtt ttaaatgtga tagcattcac acctcaacaa gtgcaagcaa tacaagcttc
     2941 tgatagttct tatgtatcta tcagctggtt atctcctata caatatcttc ctgaacttga
     3001 agaaagtttt gaaggtgata ttttccctcc ccctgaatgg ttacaattta taaataatgc
     3061 tgttcaaata aataattacg gtgtttcaac cacctggggt aaagtaggaa tggttcattc
     3121 ttatcctccc ataattcctc atagtggttc ttatcaagta ggaattgtac ctagtcaatc
     3181 tgaccaaaat gaatggttaa ttaccccgca atttacatgt cctgaaaatg ccattttaac
     3241 tttctggagt tatgtctttc tgggagatca aggggaggga ggttgtaatc ttatgatttc
     3301 tactaataat gggaatggtt ggttatcaat ctggaatgca aaatctaaat atggcggctt
     3361 aaacaattat gaaaaaccaa ttaccgtaaa tctttcaaat tattctggaa gacagattag
     3421 acttgcctgg atttattcca gtccacaaga atatggatgg tttctggatg atattacagt
     3481 ttctaattct tctattgttc ttaaatttcc cttagaatct ataaaaacaa gtgatataaa
     3541 ttcctctact attataaaac ctgattttcc agaatcttta tcaaatgcta aagataatga
     3601 aagaatatta attggttacc agatatggcg attgccttat ggtacagaaa ataatcctga
     3661 gaactggact ctacttacag aaacaccaat tccggaatta agttatcaag ataatagctg
     3721 gaattccatt cctgatgata tttatagatg ggcagttaga tctgtttata ctaataatat
     3781 acaatcaaat ccagtctttt ctaatgcctt atctcatggt tttcaagctg gtgtcatttc
     3841 aggaagagtt atttcgggaa ccctctacta tagttttcct gtatttaaag caaccatatt
     3901 ctgtggctca aattctacta ctacagatgt tgcaggttat tataatttaa tagtaccttc
     3961 aggaacctat aatgtttcag cttctgctcc gatgtgtttg aaccagacaa taactgatgt
     4021 aaatgtaaat ccaaatcaaa ttactgaagt gaactttatt cttccttatg gttcttattc
     4081 ctcttttgat aattttgaga gttacgaaga ttttgcctta atttttgaac catcagccga
     4141 atgttatatt ttaagaacca tagatttaga ccatagtcct acctgtacat tagaaggtgt
     4201 aaactggcct aatgctggat taccaatggc ttttatggta tttaatccct ttgcaactat
     4261 tccacctcta tcaggagctt taccctttag cggctataaa gaagcggcat gttttgggag
     4321 cgtaaataat cctaataatg attggttaat agcatattct ccttcaaatg ttggatttca
     4381 attctgggct cgttcatatt caccagataa tcttgctaga tttaaggttg gaatttcttg
     4441 gactgataat aacccttcaa attttactat tatcagtgga gaaaattata ttgaagctcc
     4501 ctatgtttgg actaactatt cttacaatac agaagctcca tattatggaa ttcagtgcgt
     4561 atcagaaaat ggagctattc tattcgtaga tgatttgcaa aaaggatttg taaccataga
     4621 tgatcctatt gctactcctt ctgcatcaga aattttaggt aattatccca atcccttcaa
     4681 tcctgaaact actatccgtt ttagtgttaa agaaacctca tctgtgatcc tggaaattta
     4741 taatcagaaa ggacagtata taaatacctt agtaaatgag actaaaacac caggtaatta
     4801 cagtgttatt tggaatggaa aagattataa taatagagct gtatccagtg gaatatatat
     4861 ctgtcgtttg aaagttggta agaatataag tacaagaaaa atgcttctaa tgaaataaat
     4921 tgaatatatc tatattataa tttactttaa taaaatagat gtaattcctt atattttata
     4981 gagaagctta taagattttt aactctctct tagctcttcc ttagctctcc cttagctctt
     5041 tcttagcaaa agagtcaaga aagagatagg ggagagaaaa gatagtctta ggagatataa
     5101 ttctgtgaat ctgaagttca gtaaaatctt aaaaagaagg agttaatctg gataaacatt
     5161 tttctcttga cagaaataat caattttaag atgagtatca aataattaat tgaacaatgc
     5221 tgggggcgta gttcagtttg gttagaacgt ctgcctgtca cgcagaaggc cgcgagttcg
     5281 agtctcgtcg ctcccgccat tttagaggat gctaagaaaa gcatccattt ttttataaag
     5341 aaagtttata atatgacgag attcctcttt cactttttta atattctatt agagatttgc
     5401 tgtctgttgc tcagtccttt aatcttaatt tttataggaa taagaagata ttggggggca
     5461 ctcaatttca aagcaacaga tagtgaaaaa ggaataatga tacattctgc ttcagtagga
     5521 gaagttaatg ccgttcgtcc actggtcaaa gctcttcaag aaaaatatcc aagactaaaa
     5581 atagtgatga cttgcaccac tttaagtggc ttagagcaag ccaaaacatt gcatattcct
     5641 gcttatttat cagttctgga ccttccttgg ttaaggaaaa agcaattgca atctatcaat
     5701 cctcaaatca ttatcattgt ggaaacagaa atttggccca atttgcttta tcaggctaaa
     5761 aagcaaggaa taaaaattgc ttttgttaat gcacgtctat cagaaaaatc ttttaatcgc
     5821 tttaaaaaat ggaagaaatt actgcacttt ctggaagaac ctattaaaat tataatggca
     5881 caaacacaag aagatacagt tagattcagg aatctgttta cggtccctgt cctttatgtg
     5941 ggaaacctaa aatttgccct acaattgcct cagtatgatg aacttgaact tagaatgaag
     6001 tatggctata aaccagagga ttttatcatc tgttttggga gtagtagacc aggagaagaa
     6061 gctttattaa aaaatatctt acccgaactg aagcttcgca taccagaatt aaaattggtt
     6121 attgctattc gtcatcccta tagaattgga gaagttagag accttttccc ggaagcagca
     6181 atttattctg aacttcaaga gaatgtaaat gattcgggtg atgttctgtt aattgatacc
     6241 ataggacatt taaatgagtt ttatgctatc tgtgatttag cgattgtggg aggttccttt
     6301 tttgattttg gaggacataa tcctctggaa ccggcttttt atggaaaacc aattataatg
     6361 ggtccatatc atagttcttg tgctggttct gttcaagagc ttttctcctt taatgctata
     6421 ttgatttcgg attcgcaaaa cttaaaaaga gatataatat atctttatca aaatccagaa
     6481 aaaagaaaag aaatgggtga aaatgccaga gcttgtatag aacagaataa aaatgctctg
     6541 gaaaatcatc taaggggttt agaaccatgg ataacagcat agaaagagaa gcaatgttct
     6601 atgaaaagct tgataatcag caagttagat gcctactttg tccgcacgct tgtcttttaa
     6661 aagtggggga gactggcact tgccgcggtc gtaaaaatat aacaggaaaa ttgattgcta
     6721 taaattatgg gaggacaatg ggaattagtc ttgatcccat tgagaaaaag cctttatatc
     6781 atttccgtcc tggttctcag attgtatcct taggtcctaa ttcctgtaat ttaagctgtt
     6841 ttttctgtca gaattatgac agcagtcaga tgccctgtcc aactgcatat atcaaccccg
     6901 aacagttata taacctggtt ctggaacatt cttatcaagg ctataaacag gtagctttca
     6961 cctatacgga accttttacc tggtatgaat atatctatga ctttgctaag cttgccagag
     7021 atacagatat agtaatggta acaaatggtt acattaatcc tgcaccatta aaggagttaa
     7081 tgccatatat tcgggctatg aacatagatt tgaaatcaat tcgcaatgaa ttttatgtta
     7141 aacattgtaa tggaggtctg gagacagtta aacagaccat taaaaatgct tttaatgctg
     7201 gaattcatat agaactgact aatcttctaa ttcctacctt aaatgatgcg gaagaagata
     7261 ttcaagcttt aattgatttt gtttcctcta taagtgtgga cattccttta cacttttctg
     7321 cttattatcc agcatataaa tctaatattc caccgacttc ttcacgcatt gttttaaaag
     7381 cttgtcaaat ggcaaaagaa aaactttcct atgtttatgc tggaaatatc tggtctaacg
     7441 aatttagaga aacccattgt ccccaatgcg ataatcttgt gatatctgct cacagaagga
     7501 tagtaggaat agatgataaa ggacaatgtc gtaattgctc ttataggata tatggggttt
     7561 tttgatgttt aaatggctca aaagccatct aagctgggca gattttatct tgattttagt
     7621 ggtaattatc gctatcatta taagtggtat actggtggta aaaaaagaag atgaaagaaa
     7681 tgtgtatatt tataaaaata atcaattaat tggagtttat tcccttgaac aggaccgtat
     7741 tatccgaatt gacgaacata acactgtaga gattaaaaat tcaaaagtga ggatgttata
     7801 tgcagattgc ccaaatcata attgtgttag acagggagcg ggtgatattt tgcctataat
     7861 ctgccttcct aataaagtag tggtagagat tcattctaaa aatgctaaaa gacctttgat
     7921 tgttcaatga tatccctatg ctaaaatttg ctttttatat atctcatcat ggttttggac
     7981 ataccactcg tatggcagct ctggcaaaag aattcaatga atttgatata tttgtctata
     8041 tccgaagtgc taaaccagat tatctttttc aagatttaaa tccacattta tatgaaaaag
     8101 aagatacaat ttgtgatgta ggggtaaaac ataaagcgaa tttggaaccc gataaagctg
     8161 ccacccgtct tgccttgcta caattaatgg gtaaaagaca ggaaataatt gatagagaag
     8221 tggattttct cagaaaagaa aaagtggatt tgattattac cgatattcct tggttacccg
     8281 ttgaagctgg cacctatgca gaaattcctg tttttgctat atccaatttt gattggctct
     8341 tcatctatga taagttattt ggcaaacaga cagatttaaa gccggtgctg aatactattt
     8401 atggtcttta tcaaagagta gattatgctt ttagattacc tctcagctca actaaaagta
     8461 tgggttcatt cagaaaaata gaaaaaactg gtttacttgc agcttataaa cctccaaatc
     8521 cagaactgaa aaaggctctg ggtattgatt caaagattcc agttttaacc tgctcttttg
     8581 gaggagaagg ggagatgaat ctcaactgga aaaatatgtg ttccgctttt ccaggaacgg
     8641 ttatatctac cagagaatta aaagaaattc ctaactatat ccagatacca ccagattttg
     8701 atttctcttc tttaatcagt atatcagata ttcttttaac aaaaccagga tatggaagct
     8761 ttgctgaagc tatccaatca ggaacttttt tgatatacta tccaaggaaa gattatcctg
     8821 aagaagaagt cctaattaaa ggtatatctt cctatccaca aaaaatccaa ctacctgaac
     8881 taaatctaag tgtctcagaa tggaaaaatc tttttcatac tgctcttacc ttttcaggtc
     8941 acagaaaaat aatacctaat agaaataaac aggtggcttc acttatcctg caacgctata
     9001 ttgaattaca ttattctcaa aagaaactga actctatttt tgatataggt tctaataatc
     9061 tgaattatgc tctttgtgaa gcaggaaaat ctgttccggt tcatactgct caaatcaaaa
     9121 ctggaatagg tagagaatat aaaatagtaa aaggaacagt aagaattaaa agagaagcta
     9181 ttaaaagatt caaatctctg gtcagtaatt ttatggcgta tgaccaaaac ataccttctt
     9241 ccaaatttgt tatagcaacc ggaatacatc gccagagtcc acaacttcaa caattatcag
     9301 agtggtttaa taaaaaatgg aaagctaagt ataggttact taaagaagga gaagaagctg
     9361 aattagctta tctggcagct aaagatttaa ttcctgataa acaatctgct ataattattg
     9421 atattggagg gttcagcact caatttatct actctgaacc tgattcaaat atagatagaa
     9481 tgagcatacc tattggtctt ttgactattc gcaaaactgt tcaagagggt aaaaaactca
     9541 acaatgtcct tgataaaata gcgaaaagca tccccttctg gaaagcagat atgattatct
     9601 gtgtgggact aactgcaacc tttttagcta agattgtaaa aagaagtaga tattatcgtc
     9661 cggatgaact tcacggttgc agaatttcct tgaaagaatt gctaactttg aaagatattc
     9721 ttgaatccgg caaaaatgaa gaaatagcga attatgctat ggaaccagaa tctctggata
     9781 ttttatacta tagcattcaa tattatatat ttctgttgga cagattacag agctcaggtt
     9841 ttttggtatg ttattatggt atagctacag gctacaacca gaagttaaat aaaataaata
     9901 aacgataata agatgaaatt ttatatagaa acctacggat gtcagatgaa tattgctgat
     9961 agtgaattag ttagttccat tttaatggat gcaggacacc aattagtcag taatattgat
    10021 gaagctgagc tgataatttt caatacctgt tccgtccgtg agcatgcaga aaatcgtgtt
    10081 ctcggtcgca tagccacgga aaaatataga aaaacaatca atcctgaatt gaaaatagcg
    10141 gttattggtt gtatggctca aagaatgggg aagaggttga tagaaaatta tggaatagat
    10201 tatatagtcg gtgttgataa gtatctggaa cttcctgaac taattcagca agaaaagggt
    10261 gtaaagacag aaactgataa tcttcaaacc tatacaggaa ttatgcctct gcatcaaagt
    10321 caaacttgtg cttatgtaac tattatgcgt ggttgcaata acttctgttc ctattgtatc
    10381 gttccgtatg tgcgtggaag agaaagaagt ttacctgccg aacttatcct tgaagaagtc
    10441 aaaagatgtg gtgaaatggg attgaaagat atcaccctgc tgggacaaaa tgttaattct
    10501 tactcttatc aagatatcaa ttttcctaaa ttattaagac aattgaatga tatagattcc
    10561 atctatagaa tcaggtttat tacttcccat cccaaagacc ttaccgatga acttatagaa
    10621 gtgatggcag aaagctctaa gatatgtgaa cacatacatt tacccttaca aagtggagat
    10681 aatgacatcc ttgcagcaat gaatagaaac tataccattg aacactatct aaatatcgtg
    10741 gataaattgc gtaaagctat accagatatg gcaattacaa cagatataat gactggattt
    10801 cctggagaat cagaaacagc ctttaatcat actttgcaag ctatgcaaga gattcaattt
    10861 gattatgcct tttgctttaa atacagttca agagaaaata ctaaagctgc ctatttacca
    10921 gaccaggttc ctgaagagat tagattagaa agattaaaaa gaatggttaa tctacaaaga
    10981 caaatatccc ttaaaaagta tgaagctaaa attggttctg aagttgaggt ttatgtggaa
    11041 agtnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnctatttt gtggagttat
    11161 tatgaaaagt atgactggtt atggaaaagc taaaattcaa aaaaacgata tggattgcga
    11221 aatagaaatt aaatctatta atggtcgttt tctggatatg agaatatacc tccctcgtga
    11281 aatcagtttt tttgagtatc ccatcagaca acatattggt aaagtactca gtagaggaac
    11341 agttgaaata agggttactt ttgctgatcg tagagaacct gccttaaaat taaatgaagt
    11401 aaaattagtg aaatattatg agttagttaa acaagcacaa gctttacttg atatagatga
    11461 agatatctca ttggaatatc tattaaatga gcctggtata attgaatcta ttaataatct
    11521 ggatgaagat gaacaattag ctgaattatt gaatcagact ctggataagg ccattgaagc
    11581 attagagtta tccttaagag cagaggcaag taatattaaa gaaacattaa ggcattcttc
    11641 tgatttgatg ttaaagactt tagacaacat agaaaaggag atacaacctt ttaaagaaga
    11701 attgtattct atattaaaac atagagttac tgagttatta agtaatttca atgctgaaat
    11761 gttggaacaa agattagttc aggaactcgc tatttatatt gataaatatg atatccaaga
    11821 agagatatca cgcttgaaat gtcatattaa taccttatta gcaacgcttg aaaaagaaga
    11881 agatacaggt aaaacgctta attttattct tcaggagatg caacgcgaag ctaatacttt
    11941 gggttcaaaa ttctcaacag ctcgcacatt tacctatgtt ttgactctaa aagaagaaat
    12001 agaaaaatgc agggagatag ttcaaaatgt cgcctgaacc attattaacc tggcgttcct
    12061 ggccctttat agaaagacct ttcacttctg tattattaat tctctttctc ctttttctgg
    12121 cttattttct ctggaatgta acagtggagg aatggcatca gccctggtat tattatctcg
    12181 gtatgttgct ggttataggt acccttctcc cctatttcat tcccacaact tattatcttt
    12241 atgaaaatga aatagttata atgtatctct ttatcaaaat aacgcggaaa tactctgatt
    12301 ttggatgttt ttattgtgat aaacgtggtg tgatgctcag cacttttaag atgccaagga
    12361 gacttgaccc atttagaggt caatctttac gcttttccaa aactcaaact gagaaagaac
    12421 aattattaga aatcctgcga gaaaaaatag gaaaacagta ttgaggtaaa cttgaagaat
    12481 acaaatctca gtgaccgtat agaacaaaca tttattgaaa ataacaatac tcctttatct
    12541 tataatgagt tagttaatga acttcattta gtcaggcgag aaaaatcatt acttgcagaa
    12601 actttacagg caatggtggc agaaggtatc cttaccaaga agaatagaaa attccgatta
    12661 gctgtaagtc ctgaccagct aaagaaagaa acctatgaac cagtttctca tcccagattg
    12721 ctggaaggaa tatttgatgc tactcctctg gctcgtgact tatcctatgc ctttgtccgt
    12781 acagaacagg gcgatttcta tgttaatgca gaagacactt tgaatgctta tcataatgat
    12841 atagtagcaa tagagccaca ttttcgtaag ggtaaaccag attatgctat tgtccgcaaa
    12901 atcataaaac gtgctaatga gactatgact ggtgatattc gtgaaagtgg gagcagaact
    12961 ttctttataa gcatcaatcc taagattcat aactggatag aagttagtga cttaaatggt
    13021 gcaaaagaag gggataaggt tgtattggag gtaacaaatt ggggcaaccc tataatgggt
    13081 agaatgccag taggaaaagt aatagaaatc ttaggacctt caggagaccc ggaagttgaa
    13141 cttttagctg taatcaggca atataatctt cctttacagt ttcctgatac cgttatttct
    13201 gaaatggaag caatagaaga atccatttca gacaaagagt tagcccatcg cttagacctc
    13261 agaaacatat taacctttac gattgaccct gcttcagcca aagattttga tgatgctata
    13321 tccctggaaa ggattaatca aggctggcgt ctttatgttc atatcgcaga tgtagcacat
    13381 tatgttaaac ccgaaagtgc aactttttca gaagcagtga agcgtggaaa tagcttctat
    13441 tttcctaaaa aagttattcc catgttacca gagaaacttt ctaacaaagt ttgtagctta
    13501 cgtcctaatg aagataaact ggcaatgagt gttatcacag attttaatct gcaaggcaag
    13561 attatgcgtc aaagattgga agaaagtgtt ataagaagtg attatcgtct aaattatgac
    13621 caggtagatg agcttttttc cggaaagaat actgattttc cttcagattt atcagaaact
    13681 ttattgtcag ctcgtaaact ttcttctttg ctaacagcca aaagacttaa agaaggttat
    13741 atcttttttg accttcctga gctggaatat gaatatgatg aagaaggatt tcttaaaaga
    13801 tttaccttag cagaagaaac agaatcgcat aaattgattg aaaactttat gctggtggca
    13861 aatgaatgta ttgctacaaa actatctaaa cttgctcccg ctacaattta tagaattcat
    13921 gaggacccag attgggaaaa gattgaaaga ttaattgaat tgctatccta ttacggtatc
    13981 tctttttatg aaagagaaaa tcttaatgct tccattcaat atttactgca ctccctaccc
    14041 actccagaac atcacaaagt ttttgaaccg ataatcctgc gaagtatgaa aaaagctaaa
    14101 tattccactg aacatataag acattttggc ttaggaatgg aaacttacac tcattttacc
    14161 agtcctatca ggcgtttatg cgacctggta attcaccatc taagcaagac ttatatccta
    14221 aaaaccagta agatacaatt tgattatagt caattgaaac actttgcttc tgttgcatct
    14281 gaacaggaat tgcaggcaga tcaatcagaa agggatattg aacattattt cagtatgact
    14341 tatatgaaaa agtttatcgg agaaagcttt agcgggatag ttatgtctgc caaaaatgat
    14401 agactcatca taagattaga ccagataccc atcaatgcag tcttaatggt cacagaattt
    14461 cctcctggaa actggatttt tttagataga caaatgcgct ttataaatga agataataga
    14521 gattattttc agctaatgga taaagtgctg gttaatatta tggaagttag tgacgatatt
    14581 tatctggaac ttaagccagg taaggaatca catcggcatt ttcaatttca gctttctaat
    14641 aaaaatagtt tcaataatga taataggata agaaaaaacc agagaggaaa gaaaagattt
    14701 gatgttgact ataaatttag aaataaaaat tatggcaata caaataagag atatagagga
    14761 aagtgatgaa acaacctatt ggaatatttg attctggagt gggtggttta actgtctata
    14821 aagccattaa aagagctttt ccagctgaaa atatcatcta ttttggtgat actgctcgtg
    14881 ttccctatgg acctaaatcg gaaaatacta tcatagaata ttccattcaa aatgctcgtt
    14941 ttcttcttca aaaagacatt aaaatattag tggtagcttg caatacttcc tctgctgttg
    15001 ccttacagga attggagaaa ctaacagata ttccaatcat cggagttatc aagcctggag
    15061 caaaattagc tgcagactca actcataaca atcatatcgg aataattggc acggaaggta
    15121 ccatccgttc cgaagcctat aataaagcca ttaaaaccat ccttcctgaa gcagaaattt
    15181 tttcctgcgc atgtccccta tttgttcctc tggttgaaga aggctggtta aatcatccgg
    15241 ttagtagaat ggtagcagaa gaatatctta acttttttaa agataaagag atagacacac
    15301 ttgttctggg ttgcactcat tatcctttac ttaaatcttt gattgatgaa attatggaac
    15361 ataaggtaaa acttatagat agtgccgatg ctattgcctt gcatcttaag actttgcttc
    15421 cagttgaaag tagcactgag cctgtaatgg atagatttta tgttagtgat aatgaagcaa
    15481 agttttccca aatcgctgaa tacattctgg gtgttcagcc tacgcctatt aaaagagttc
    15541 gtttatatga gagctggttt gaataaataa caaaggagaa taaaatggaa cccttaatct
    15601 taaccgcagc aattactgga gcagaaactt gccgtaagga tcaacctaat ctgcctataa
    15661 ctccagaaga acaggcagct gaagcattag cttgttttga agctggagca aggattatcc
    15721 atctgcatgt gcgtgatgat gaaggcaatc ccactcagag tttagagaga tttgaagctg
    15781 ctattacagc cataaaatca gctgttcctg agattatagt tcaaatcagt acgggtggtg
    15841 ctgtaggaga agattttgaa aaaagattag ctcctctatg cttgaagcct gagatggcaa
    15901 ctttaaatgc tggttctttg aattttggca atgatgtctt tatcaataga ccaccagata
    15961 tagtaagatt agctgaagct tttaaagaat atcaggtagt tccagaggtt gaagtatacg
    16021 aatctggtat gatagattat gtagcaaaat tgattaaaaa aggagttatc actcattcac
    16081 ctttacatat tcagtttgtt cttggagttc caggtggaat gaatggttct cctaaaaatg
    16141 tagtctatat ggcatatcat ttaaaggaac taattccgag tgccacctgg gctgttgcag
    16201 gtattggaca gtatcatata ccagcttctt taaccgcaat ggttatgggt ggacacatca
    16261 gagtgggatt tgaagataat atctattatc ataaaggtgt tttagcagag tctaatgctc
    16321 agttggtggc acgtatggca agaattgcag ctgagattgg aagacctctt gcaactccag
    16381 ctcaagcacg agaaattctt ggcttatagg tgaattgaaa tggaattaag tgaaaatgcc
    16441 ctgattgttc tggagagacg ctacttcaga aagaatgaag caggacaaac tatagaaaac
    16501 tgggaaggaa tgataaatag agtagcgtcc aatattgctc agggtagtaa agataaggaa
    16561 aaactctatt ttgaactttt agattccggt atgtttttac caaattctcc tactttgatg
    16621 aatgctggaa gtgatttaca acagctttct gcctgtttcg tgcttcctat tgaagatagt
    16681 atggagagca tctttgagac tttgaagaac gcagctttga ttcataaaag tggtggagga
    16741 acgggttttt ctttcaatca tttaagggaa gcaaatgctc ctgtccgttc tactaatggc
    16801 gtttccagtg gtcccatctc ttttttgaaa gtatataatg ctgctacaga tgcagttaag
    16861 cagggtggaa ccagacgcgg tgccaatatg gcaattctca atgtagacca tcctcagatt
    16921 ctggaattta tccagtgcaa agcggatcct aaagagctta ccaacttcaa tatcagcgtt
    16981 ggtgtttcag aagcttatat gcaggcagtc ttaaatgata atgattacga tcttatttct
    17041 cctcatagtg gtaaagttat tcgtcgtcta aaagcaaggg atgtgttcaa tcttattgta
    17101 gaaatggctc atagaaatgg agagcctggc atcatctttt tagataaaat caatgctgct
    17161 aatcctactc ccaagttggg caggatagaa tccaccaatc cttgtggtga acaacctctt
    17221 ctcccctatg aagcttgtaa tctgggttca attaatctgg caaaatttgc caaagatgga
    17281 agcattgatt gggataaatt gcaatcagtt atccgactca gtgttgattt tttggatgat
    17341 gtgatagatt gttctcgttt ccctttacct cagattgatg ctttagttaa agccaataga
    17401 aagataggat taggagttat gggttgggca gatttacttt ttcaactgga aattccttat
    17461 accagtgaag aagccatctc tttagcgaaa gaattgatgg aatttattga ttttgaagca
    17521 aaacaacgct ctatggaact ggctaaagaa aaaggtagtt ttcccaactt tcctgattct
    17581 atctatgaca ctttatcttt aaaaagagaa cctataaaaa aggactggga taagcttctt
    17641 tctgatataa aacaaaacgg aattcgtaat gctacagtta caactattgc tcctacggga
    17701 actattagta tgatcttaga tacttccagt ggaatagaac ctcttttttc tttagtctat
    17761 gtgaaaaatg taatggatgg tgagaagcta atctatgcca ataaatattt ccagactgcc
    17821 ctggaaagag aaggtctaat ctcacctgaa cttttatctc aagtctctca aactggttct
    17881 ataaaagaca taaaggaaat tcctgaaaga tttaaacaga tttatcaaac tgctctggat
    17941 ataaatcctg agtggcatat aaggatgcaa gcagcttttc aaaaatatac agataatgct
    18001 gtgagcaaga ctataaactt tccgcattct gccactattg aagatatctc taaagcttat
    18061 caacttgctt gggaactggg ttgtaaagga ataaccgttt atcgtgatgg aagcagagaa
    18121 gaccaagttc taagttctgg tattaaaatg actgtttctg aggggaaagt ggcacctcgc
    18181 agtcgtccag atgtaactta tggtttaact caaagattgg aaacgggttg tggtcatatg
    18241 tatgtaacca ttaattctga tgagtttggc gcttgcgagg tcttcattca aatgggtaaa
    18301 gttggaggtt gtgcttccgc tcaattggaa gctattgcac gattatcttc tttagctctt
    18361 cgttctaatg tgaaattaga atctattatt cgtcaattaa aaggaatccg ttgtccttct
    18421 ccaatgtggc ataatggaac tcttatcact tcctgtggag atgcagttgg acaggctttg
    18481 gagaactttc ttaaaattca taacaatctt caggtggaat catttcatat ggaaactgaa
    18541 acagatagcc acatagaaga acctggcgca aaaagtgcta tttatgtttg tccagattgt
    18601 ggttcaactg tccaacatct ggaaggttgt ctgaaatgtc ctgtttgcgg ttggtctaaa
    18661 tgttgatttt atattttagg agtagtaatg cgttgtaaac attgtgatgc acgtttagca
    18721 cctcatgatt tttggtgtgt gaattgtgga aaacaaaccc ctattgtagt gcatgaattg
    18781 gcatcttggg gaagttttaa aagaactttt aaacaatact ggcagtttaa aggagccaat
    18841 attccagcag cagcttttgc tatcttagtt ggagttattc ccatcatagc ctttttagta
    18901 ttgttaaatg cttttgggat actggatttt caagcacaag agactgcagg aaagctgcta
    18961 ttaaatctct ttatcactgg tataggtctt agtttattga ctcctttatt attgatagct
    19021 tttaaaccgg tttgtgaaat caaggattat acactcactt taaaggattt attaaaagga
    19081 tttaaagcct atcccagata ctttttcttc tctttgataa cggctctata ctttatcctg
    19141 atttatatca tatgtttcgg attaccaaat tttgccagtg acccaattct tcatctagtc
    19201 tggttagttt tagttggata ttatattggt gtttctttac cagtacccat ccttatggaa
    19261 cgcaagaagg tcaatgcttg gaaagcaata agaatggcat ataagtattt taaagtggta
    19321 cgctggcaac ttttctggct gggacttatc ttactggtat taaatcttgc tgccacagct
    19381 ttagctctgg tctttctctt aataacttta ccattatcct ggtttgctat tagagattac
    19441 atagatttat tgctggagta tgagattatt cgtgaatata agactaagtg aaggatatta
    19501 aattgactaa gaaagaaatt atctctctca ttttattaat cgtggtagtt ggttatggag
    19561 cctggacctg gataaatcgt tcttattctg aaactaagag agatctggat ttactggata
    19621 cggtggtaac tatctctgct acttctaaaa gcaaagatat tggcactcag atagattctg
    19681 tctttgccta tattcgtact tttgaagata tttttgatga ttataaccct caaagttggg
    19741 tttataaagt caataatgct cagggcaaac gctacccgat gaatcctgat gcctatgaac
    19801 tactctgtgt ggcagatagt ctttatcagc ttactaatgg agcttttgat ataactatca
    19861 aacctcttta cgacctctgg ggtttcagta aaataaatga atatggaacc gatactttaa
    19921 gtttagcgcc tcccgattca atgttgattc aagaaacctt aaagaatata ggttttaatc
    19981 gcattcgcta taataaacaa tatatagaaa tgcctcaggg tatgcagatt acctttggtg
    20041 cattagctaa aggttatgct ctggataaag ctcgcaatta tatggaaaat cataaattta
    20101 ttagcggtat catagattgc accagtagta tgactttatt tgggcagaaa cgttctcaga
    20161 ttgtgagtat aaaacatccg agagctaatg ctggaatgca aacaattggc aatttcaaga
    20221 taaaaaatgg ttctttaggc acttcggggg attatcagct ttattttgag tataatggac
    20281 aaagatatca tcatattcta aatccttata ctggttaccc tgtggaacat gtttattcag
    20341 tgacggttat taatccttct gccacttggg cggatggact ttctactgcg ttgtttttac
    20401 tgcctccgga ccaagcaata gaagcattaa aatattatcc tgatagtaat gcagttatct
    20461 tctatcaaga aaatgaccag atagtatctc ttaaaacctt aggaatgaaa gctttggatt
    20521 ggcgcgacga acaatgagca tacctgatat acaatcccaa gacgactttc gtcaaatagc
    20581 aattgaaaaa gttggagtca aaggattaag ttatccaatc gtagtagatg atagagaaaa
    20641 tggaactcag gatacggtgg gaaacatcaa tttatatgta gacctccatc ccagtagaag
    20701 aggtatacat atgtcccgtt ttattgaagt tttaaatcgt tatcatcacg atagtatcat
    20761 tggcaatctg gaatctttac ttcaagacct aaaatatata atgaaagcag aagcagctta
    20821 tattgatatt tatttccctt attttattaa aaaacttgcc cctatctcca aaattccttc
    20881 tttgatgaat tatcaatgtt tctttaatgc ttcctttaat aatgcttttc aattctggat
    20941 tggaactact gtcccagtta caactctttg tccttgctcc agagctatat ctacaaatgg
    21001 tgcacataat caaagagctc tggttacaat aaaggttaga tatacagagt tcgtctggct
    21061 ggaagaacta attcaaattg cagaagaatc agcaagttca cctgtttatc ctcttttaaa
    21121 acgtagagat gaaaaatatg taactgaaaa cgcttatgcc aatccgaaat ttgttgaaga
    21181 tatcgtgcgt gaagtgaccc aaaaactgga aaaagactca cgtatcgtag aattcattat
    21241 tgaagtggaa agtatggaat ctattcacaa tcacaatgcc tatgctatga ctcatagact
    21301 taaacattaa agttttggat taaacccttg tcagaatcac ttgtaaaacc tgcagtttcc
    21361 ggttttttac ctaacaattt aaaatacata taccagccag gaaattttga gccagtcatc
    21421 tgtttacagc tatacatcaa aataggtagt gcttgggaaa aggaagatga agctggcttt
    21481 tcccatttta tggaacatct aaccttcaaa tcaactgtca aatttccctt caatcagatt
    21541 gcagcttata tcagcaagtt aggtggttct atcaatgcct acacagattt tgattgtacc
    21601 tgctattata tttcactacc ctcagaattt gttaatgaag ggttagaagt tttagctgag
    21661 ttggcttttc gttcaacttt taccaaagaa gatgtggaag ttgagaaaga tattattctg
    21721 gaagagatgg ttcagaatac cttagaccct gagaccaatt ttctgcagtt tgttcaggat
    21781 gctgctttca ccaattatcc attaaaaaga cctattttag gaactaaaga gtccataaag
    21841 aaagcatcat ataaagaact aaggagtttt taccataaat actatcaacc gcataactct
    21901 tttctggtta ttgccggaga ggcagaattt aatcaattaa aatcaataat tgaagaatta
    21961 tttggcgatt ggaattcaca ctcaaaactc attttcccag attttaaaca atttttagaa
    22021 ccaaattttc ctcctcttcc ctatctctca aaatccagtt ctcagttatt tatagctttt
    22081 gttctacctg aattgagtga taaacatcct ttaagtaatg ctatgcttat agccatccgt
    22141 tatttagcaa ttggtcgttc ttctcgctta tttaaaagat tggtagaagg ggaaaaactt
    22201 gcctctgctg ttaaagtatc ttccatttct ggtattatga ctggagtttc agctattgta
    22261 gtgtatccta taaatgaaaa gtccattccc agaataataa atatattcca ggttgaatat
    22321 cagagcatat tgcaaggtga actggattta aatgaaatag aactcattaa aaaggatatt
    22381 atcaatacct ggcgttatgg ctttgagtgt atggaagacc tggcagagat gattggtgat
    22441 gaagagttta ttgaaggtta tgaaaatctt tataactatg acgaaaaaat agaacctctc
    22501 caactggaag atatcaagaa agtaataact caatactggc aaccttctta tctacaaatc
    22561 attcaacaga caccccatcc aggttctcta tctcttccag agaaattatc ttataaaaaa
    22621 gcaaatatca cttctcctat tcctgaatta aaaattcctg cttctttaac tcatcctgta
    22681 ccttataaat ctattagacc tgatttttat tctgtcaaat tagcaaatag actttcatta
    22741 ctatatcgct atttaccaaa tcgtcctatc agtggttttg ccttagcaac ggatatctgt
    22801 cagttgaacg aagagaaaaa tcagcacggc ttaaattatc tatgttcttc tgctttgcta
    22861 cattcttcta caaattataa tgctgaagag attctttctc tttaccgact ttatggagtg
    22921 agtttaaagg ttgagcattc tctggatact actatatttc gtggtaaatg ctttcataaa
    22981 gaactgttgc ctgctttaac tttactggaa gagctaatct ttcatccagc atttgaggaa
    23041 caatatatta aacttttaaa aaccgctgcc attgatatgc tacgaagaga taaacaggtg
    23101 ccctacaacg aagcttttta tcattggttt tatttactct ttggaaaaaa tagtccctat
    23161 ggtcgttatt ccggtaatat ttcagattta aaaaggcata atttagaatc agtagtaaac
    23221 tggtataaga caaattatcg tccggaacat tttagtcttg tagttatagg ttctttagag
    23281 ccggataaat tctttagtat cacacctcaa atttttaagg aaccagctcc ttcagaaaat
    23341 tggacaggga aaaatccgga acctcctcat cccataaaaa agcataagat tatcgctcat
    23401 aaaagttctc ctcaagctat tattcatctg ggtggttttg cacctgcagc taaagataga
    23461 gttgatagca ctgcttttta tctactggct caaattattg gcggtgatat ggattctcgt
    23521 cttttcaacc ttgttagaga acaaaaaggc tttgcttatc agacgggatt tgaatataac
    23581 tgtgctcata gcataggtta ttggtttgcc tatgcttatt gtgagccaga agtttataag
    23641 caatgtcttg aacttatact ccagatactg caagaagtga aagaaaatgg agtaaccgag
    23701 acggaactgt taaatgctca aaattatctc tgcggtatga atcgttttga agcggaaaat
    23761 gcttctgctc aagctatgtc tatttcttca ctaagtgctt taggctatga accggaatac
    23821 tattttcagc gtgaagaaag gattcgtagt gtggatttaa aaactatctc ccatctggca
    23881 aaaaactggt taacaaaaga taatatctgg atttatattt atttatgatt accttaggaa
    23941 tagatatagg ctcccgtaac acgaaaatcg tattttatga tacttcaaat caatccatcc
    24001 ttttttctgc ctggagtgct actgaagtca atcctttgca atctgtgcat tctttattaa
    24061 agcaagccca aaatagctat ggacttgacc taccagaaga gatagcttgc acgggttatg
    24121 gacgcaaact ctggaaagga aatagtaaag ttttttctga gataacctgt catacaactg
    24181 gcgttcgtta ttttatacct gaagttagaa ctattataga tgtaggtgga caggatgcta
    24241 aaataatctc tttaacgcca gagggaaaaa tcaaagattt tgtgatgaat gataaatgtg
    24301 ccgccgggac aggaagattc ctggaaatgg ttgccagtag actggaagtt tctcttgatg
    24361 aactttccga acttgctctg caatcaaaaa atcctctaac tatttcttct acctgtgtgg
    24421 tttttgctga atctgaaatc attggtatga ttgctaaaaa tacggaccct gcagatatag
    24481 catatgccgc tcatatttcc attgcaaagc gaattattac tcaattaagt gcagtagatt
    24541 tcacgcctcc cattgttttt actggagggg ttgcacaaaa taaaaacttg gtgcaatgcc
    24601 ttagtgaact tttaaatact actattatta ctcctccaaa tcctgaaatt accggtgcat
    24661 tgggagctgc tattctcatt tctcaaaccg aagaatataa ttaactcctc
//
LOCUS       DAORZF010000026        23668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_1799_length_23668_cov_349.717105, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000026 DAORZF010000000
VERSION     DAORZF010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 23668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23668
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1799_length_23668_cov_349.717105"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="PK015_04405"
     CDS             <1..404
                     /locus_tag="PK015_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33742.1"
                     /translation="LLEKIKQTGFDLYFYEGYKPHKEALNYLCGADINLVMRENNEES
                     QKALSGKIFELLRAGRPILSVGPQHSVISDIVNETQTGIHAYIEDPQAIAEALRICYS
                     RRNDPPPPKEVIEKYSREYLTKKLLSLYPDS"
     gene            complement(493..2382)
                     /locus_tag="PK015_04410"
     CDS             complement(493..2382)
                     /locus_tag="PK015_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HOL33743.1"
                     /translation="MKRFLSIFLIVWAGIFLYAVPRNLVVVEVATGTWCQYCPGAAMG
                     CHDLLNNGHPVAIIKNHNGDSFANVYSNARNSFYGVSGFPTAFFDGLNPTVGGSNSSS
                     MYSNYLPKVNARMQVPSHYTISAVGTQSNNQFQVTVTVTKPEDDTNTNIKLHAVLTES
                     NIPFVWFNQTTVENVNRLMIPDQNGTPVNLNTGESTTINLTFTPDASWNIANCEMVFF
                     LQNMTSKEILQGVKYSLPGLVGAYPVSVDHIDFPQTFIGGSSTVPITITNFLAEPVTG
                     TISINNFVFSSSITNFTIPGTQSITVDVTFIPTAATTYTGTLTITSNLYNHPVINIPL
                     TGTGFLNHAPTATNVVITGPPVIYQQLTASYTFSDIDGDAEGSSIYQWYRVIGQPHPI
                     EGANQLTYTPTEEDLNIPLGFRVVPVDEHGMNGIPVMSAFTIPIQELPPPRNLHGTYI
                     PPNTVQLTWEPPLYFDVKGLVGYRLFRDELPISTITNPSILSFSDVGVPNGTHQYWVC
                     TLFNNPMMLSGPSNVVTIVVGTAIDDPAVPAVTSVSVYPNPSMGNVSFSIQTKSNVPA
                     LMSVYNVKGQLVKQFNIITDAIGNAILFWDGTDNQGRKVDSGVYYYRLEADKDRISGK
                     LLMIK"
     gene            complement(2474..3118)
                     /locus_tag="PK015_04415"
     CDS             complement(2474..3118)
                     /locus_tag="PK015_04415"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOL33744.1"
                     /translation="MDLALIFDFDGTVANSIEAIFQLINKLAPSYGFEPLSQETFNTL
                     RDLSIPKACRKLHFPLSKLPQAITTVLNEYRQIMPQLLPCPGMDTVIRKLKEWGIILG
                     LISSNDAEHINDFLKRNNLDCFDWIEGTKGILGKHNSIRTQVKKHKLNQNHTFYIGDE
                     VRDIKAAHRSKVKIISVTWGLHSEENLLRHHPDYLVRKPEELLDLVQSIQSHLS"
     gene            complement(3124..3954)
                     /gene="rsmI"
                     /locus_tag="PK015_04420"
     CDS             complement(3124..3954)
                     /gene="rsmI"
                     /locus_tag="PK015_04420"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOL33745.1"
                     /translation="MPAGNLYLVPVPIGNLRDITLRALEILRKVEIIAAEDTRNTRSL
                     LSHYQIKSGRLISLHKYNEKKRIPEILELLEQGKDIAVVSDAGSPGISDPASLLVQEA
                     IDHQINIIPLPGATAIIPALTASGFNTESFLFLGFLPLTNKHRKEKLTLIKNSIHTVI
                     LYEAPHRIKRTLTELSEYIDNRRICLAREITKIYEEFIRGNIQDIITNYNIKEKGEFV
                     IVIEGNMRSEQIDRTEINQYIEELLGQGLSSKTIAQMVANRFRISANSAYSMVLKLKG
                     "
     gene            complement(3964..5151)
                     /locus_tag="PK015_04425"
     CDS             complement(3964..5151)
                     /locus_tag="PK015_04425"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HOL33746.1"
                     /translation="MFGKMKTDLQNMLEELKEKGLYKNERILTTPQGASIRVSTGQTV
                     LNFCANNYLGLANNPEVIKAAQDIMNDWGYGLASVRFICGTQQIHKDLEKKISEFLGT
                     EDTILYAACFDANGGLFEPILDEECAIISDELNHASIIDGVRLCKAQRFRYKHSNMEE
                     LENILKQTQNMKYRLICSDGVFSMDGDMIKLPEICDLADKYDALVMVDDSHATGYIGP
                     NGRGTPEYFGVQDRVDILTSTLGKAMGGGNGGFTTGRKEIIETLRQKSRPYLFSNSLA
                     PAIIGASLKVIDMLSGSSQLRDRLWDNAKYFREEMTKAGFDIVEGNTAIVPVMLYDEP
                     LAMKMADMLLDEGIYVIGFVYPVVPKGKARIRVQLSAAHNREDLDKAITAFKKVGKEL
                     NLI"
     gene            complement(5393..5481)
                     /locus_tag="PK015_04430"
     tRNA            complement(5393..5481)
                     /locus_tag="PK015_04430"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5444..5446),aa:Leu,seq:aag)
     gene            complement(5488..5904)
                     /gene="secG"
                     /locus_tag="PK015_04435"
     CDS             complement(5488..5904)
                     /gene="secG"
                     /locus_tag="PK015_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOL33747.1"
                     /translation="MVLYTVSLVIHIIICVALVFVILAQTSKGGLDANLGGAAMNVFG
                     GSGASKFLRKWTQILGVLFVISSLFLAFQVTKTNQAKAPKILEEQFGETSKAPAGTES
                     KTNKSVTTIPGNNEQTETKASDGSSSTQKTTEDLKK"
     gene            6206..6493
                     /locus_tag="PK015_04440"
     CDS             6206..6493
                     /locus_tag="PK015_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33748.1"
                     /translation="MKKYEVFMEFILPDGKILELEQVRKVSRIRDLGLEKDSIEYSKI
                     AFEIHLKGHKIIEVGERYHYADWAEKLKKLTTIRNNLINALKEAGIQFEEE"
     gene            complement(6547..7482)
                     /locus_tag="PK015_04445"
     CDS             complement(6547..7482)
                     /locus_tag="PK015_04445"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOL33749.1"
                     /translation="MEKIAVLEGGISPEREISLRSGEAIAKALREKGYEVIELDPADY
                     SDFYSLISKLRTEKISIVFIGLHGGTGENGELQAALSLAGFKYTGSGPKACTLTMDKY
                     VSKLMALDEGIPAPEYILMREDLLNDYQDPNDFSAIEAKLGLPIIVKPNDSGSSVGIS
                     IVEKLFELKEAVKLALTYSDSVLLEQYIPGKELTATVLDGKALPLIEIRPLNGWYDFN
                     NKYSKGHSEYLCPAPVEESVSQLIQLYAERLWKVLGLKGYARIDFRYDGNKPYFLEVN
                     TLPGMTDLSLTPMAAKAVGINFPELVDKIVSLAKK"
     gene            complement(7491..8405)
                     /locus_tag="PK015_04450"
     CDS             complement(7491..8405)
                     /locus_tag="PK015_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33750.1"
                     /translation="MSKSKYNIVARFPSKEGLSFCPLWINPDFMLAVQNLHNCEAKQL
                     VCYKGDEIKAVMPLYEKKKLGISYLICPTSSYYQGLWFYNLGKKGENRRLLDELNISS
                     DIAIWLKAHYKKMKFKLMPENYDVRGFTWKNYKAKPLYTFTYDFQEPLKLLYDEKTKL
                     SKASIYNYQLDEQFLPEEFIHLLKILYQRLNKDLGLSYRAFQKWMEDLYQHQILSQFN
                     LRREGKVVSSSLVLGGKEDDRAYLIMLSTLPEEMKNGASVVHYLTFIESLRGRFDKID
                     FCGGNNPDVARFKAAMGFKLELFFIIEK"
     gene            8710..8922
                     /locus_tag="PK015_04455"
     CDS             8710..8922
                     /locus_tag="PK015_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOL33751.1"
                     /translation="MRGKVKWFNKNKGYGFIITDDNKEYFVHWKSIVTNSPRELKVLE
                     QDEEVTFDLMETDKGTQAINIIRVNS"
     gene            8990..10657
                     /locus_tag="PK015_04460"
     CDS             8990..10657
                     /locus_tag="PK015_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOL33752.1"
                     /translation="MPFLPINLKEARARGWDELDIIIVTGDAYIDHPSFGAAIIGRVL
                     EKAGFRVGILPQPNPEKDEEFLALGIPHLFFGVTAGNMDSMVSNYTAQRKKRNNDAYS
                     PDGISGLRPDRASLVYTNILKRIAKNTPIVLGGIEASLRRIAHYDFWQNKIRASLLAD
                     TKADILVYGMAERAILAIAKALQEGKRISELTDIAGTTVFSKQPPEADEIILPDNIAC
                     QDKFTFHQMTHIFEENYLSRTIYQLNGNRWIKHNPPAESLTQKEMDELYLLPFEYAPH
                     PIYKEHKIPAYEQIKDSLTAHRGCYGGCSFCAIAVHQGRKISWRSSDSLVQEIKNITV
                     KRKHKMTITDVGGPSANMYASFCSLDFPSFCKRSSCIYPSICLNLNLAQEEQLKVLSI
                     LENLPQVKSVFISSGIRHDLAILSPQYIKALTLKYTSGRLKLAPEHCVPKILRLMQKP
                     DTSSFEKFSQIFFSTCEEAGIKRQIVPYLIVGHPGSTIQDAYKLRDWLKKHNLKVEQV
                     QEFTPTPMTLSTCMYYTGLNYWTGKPVYIPKPGEIRKQKNIILSLFK"
     gene            10848..11561
                     /locus_tag="PK015_04465"
     CDS             10848..11561
                     /locus_tag="PK015_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33753.1"
                     /translation="MKKNLLLTVGFIFFCIMAGATSIYNIQYTNSRGADNSYPSPYLG
                     KQVTVEGIVTAVNFNGEGYFLSEPVSGAWRGIYVLDTEHKPKRGNYLQISGEVAEYFG
                     MTCIRNLNSYRVLDQHRSLPTPVIISTNQLTNPLEAEAYEGVYVRLVNVSVSSIKSKN
                     GKFMVNDGSGPCSVSLGNFGTKTNLPALGTQFLQISGIVTFSYGEYTLNPVSSDDLQI
                     QQPVYIQTRSWGSIKSLYK"
     gene            11581..11961
                     /locus_tag="PK015_04470"
     CDS             11581..11961
                     /locus_tag="PK015_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33754.1"
                     /translation="MKKFFKNTLIVAGVALAAHFGVKAYKRINGVIKLSKSLGDFLNN
                     VYGVKPKISINSGINTINANNIKVEFPQEILDKYTDIETTIREYIDDFYPELAKTKIN
                     IEIQPLSEQEGENINVEVEPEPAD"
     gene            complement(12405..13814)
                     /gene="hydG"
                     /locus_tag="PK015_04475"
     CDS             complement(12405..13814)
                     /gene="hydG"
                     /locus_tag="PK015_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HOL33755.1"
                     /translation="MNIDTEGLESFIPTEKIEELLQSEQNPSESQIREIIAKSLEKNR
                     LAPEETAALLNVSDPELKQLIREGAHELKKTIYGNRIVLFAPLYVGNECVNDCVYCGF
                     RSSNKECIRATLSHEQLVQETQALESKGHKRLIMVYGEHPKYSPDFIAQTVQTVYDTK
                     FGKGEIRRVNINAAPLDIEGYRIVKSVGIGTYQIFQETYHQETYKTLHPRGPKSNFLW
                     RLYGLDRAMKAGIDDLGIGALMGLYNWKFEVMGLLYHTIHLEETFGVGPHTISFPRLE
                     PAIGTEFTENPPHKVPDEDFKQLIAIIRLSVPYTGMILTAREEAELRNEAIRYGISQI
                     DAGSSIGVGDYSAKDEESRKKSQFVLGDTRSLDDVIYELAQEGYIPSFCTSCYRAGRT
                     GEHFMEFAIPGFVKRFCTPNALLTFAEYLYDYASERTRQIGFELIDRELKQIEDENMK
                     QAVKNKLEQMEKGVRDLYF"
     gene            complement(13830..14078)
                     /locus_tag="PK015_04480"
     CDS             complement(13830..14078)
                     /locus_tag="PK015_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HOL33756.1"
                     /translation="MDEKRHIVTIVIEDREKAFNPVSELIHQHASYILLRVGYPMRDR
                     NVSVIFLIMEANLDILGSFAGKLGQIESVKVKTMTLKV"
     gene            complement(14293..15660)
                     /locus_tag="PK015_04485"
     CDS             complement(14293..15660)
                     /locus_tag="PK015_04485"
                     /inference="COORDINATES: protein motif:HMM:NF013776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOL33757.1"
                     /translation="MEFVDREEELRLIKQELSKKESQLIVLYGRRRVGKSALLQKLKP
                     TNCIYYLADINTSDIQRNFFAVQVAKLIPGFNNVLYPDWYTFFEQANRQLSKKATIII
                     DEFPNLVKSAPELPSVLQKIIDLKQNLNFHLIICGSLQQMMYSTFLEKSSPLFGRANR
                     IIKLQPLKCKWLKQILNLTSIQAIEEYSVWGGIPRYWEVRTEYNSLFEAIREAILNPY
                     GILYEEPLRLFMDDLSGAVQPISIASLIGLGCNKLSVIAGRLNKPATSLSRSLQVLID
                     AGYVKRELCWGENFKNNKKTLYRLDDPLIRFFFTFVVPNQSILNSRLIDVVEKEIKKR
                     WQVYVSETWEELCRQSVINLNVKNIQFLPAQRWWQQSKTDKPIEIDIITESVDGQNLL
                     IGEAKWGKNIKPAMLLRELDQKITYLNLQKKYQNIYRLIMLPELEFEVKENVIYAGAD
                     FVCQN"
     gene            16011..18104
                     /locus_tag="PK015_04490"
     CDS             16011..18104
                     /locus_tag="PK015_04490"
                     /inference="COORDINATES: protein motif:HMM:NF017964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HOL33758.1"
                     /translation="MSNYFMETHHHEWILTNKLGGYALGTGNLINQRKYHGLLIASDK
                     DFQRYHLVAGMEEKVEWRGQTLYLDSNNYSNCIYPEGFLCLVKPWLRPYPIFLYSALP
                     HQNDILILKELMMDEDSNTILVKYTNLGHHKLHLTLLPKYTMTPHHELNAPGSLDREV
                     FDTEFNNIDDKCFFNVVRPSNGLRVFGWMDKGEVDYKRLTYYNVFYPWEVMSGYAGIG
                     DQIAFFELNFSLAIGESRFLLFSDQPIKNPEKLINSIEKRYSALPKPKDYPTVPDKDD
                     TLISKLDFEDNFLFSYEEYIKILEFALKDFISNNDIVSGYPFYGPWGRDTMIVLNTLL
                     HMPNYLDLVEKILNKYKNQMQDGLIPNMLKESGRNTNYDTVDATLWYVILLWKLGKYK
                     QDIAFWKKSIRVIEEVLDYILTNTKYPFFIREDGLIELKEEFAHATWMDVRIDGRAVT
                     PRNGAPVEINALWYNAICCYASMCEEYTKLSGISYPPQENVIKLKDLVYESFQKFWIN
                     GYLADRLLGDEPVEEIRPNAVIALSLPWQLIDKEKMEQVWQVAYNELYTPYGLRTLNP
                     KNFRFRKKYYGTQRERDLAFHNGSVWAWLLGAFCGLYLKIHRDTMSDQELAEKLGEFI
                     STFRYSFMRGHIATIAEVWDGDHPHFPKGAPAKAISVAALYNIESYIATLKGIITPLQ
                     KKPVTRKKTLQRKRK"
     gene            18101..19438
                     /locus_tag="PK015_04495"
     CDS             18101..19438
                     /locus_tag="PK015_04495"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOL33759.1"
                     /translation="MKVLMFTWEFPPLIAGGLGMACYGMVRALLGLGIKVDLVLPTKE
                     LVYFPLREPDDVDKLPAVFLDPKKHTEYISHKFLNIEERLDYIGVTVHPETYLQLGEV
                     QKFVKAIKKEYQRESITQEEREIWEELTAHLMGDEDIFKKVQEYTIRAERFARELDFD
                     LIHAHDWLTYPAGMLARSISGKPLVVHIHATEYDRAGGPGNPRVHRIERAGMMFADRV
                     IAVSQYTAQMVMSHYRIDTAKISIVHNAFSVSDEYHRTKKRIFKGPTILFLGRITLQK
                     GPDYFLEMASKVHKVHPEAHFIMAGTGDMARELLYKSAAKKLGNKFLFTGFLNRQQVE
                     DILRASDIFVLPSVSEPFGISPLEAMAFGVTAIISKQSGVSEVVNHAFKIDYWDVDLM
                     AETVNHLIENPDLCRKIGLEGMEEVNKIHWNDSAEKIRQIYSSTLSEYLKSNP"
     gene            19450..20646
                     /locus_tag="PK015_04500"
     CDS             19450..20646
                     /locus_tag="PK015_04500"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HOL33760.1"
                     /translation="MLYVVFYFQVHQPYRLRHLNVLDIGKGVDYFDEHLNSQIMRKVT
                     EKCYLPTNKLLLDLIHRYEGQFKVAFSLTGTAIEQMKAYSPEALDSFKELVNTGYVEL
                     LGETYYHSLAFLFDTNEFLDQVAMHRDLMKSEFGYYPETFRNTELIYQDSLSDLIYEI
                     EGFHTILTEGVDRILQWRSPLYAYKNYAKTINLLLKYYQLADDIAFRFSDRDWPEYPL
                     TVDKFVNWIDRLTLSEKEGRNLFLNLFMDYETFGEHQWAETGIFDFMQHFPEEILKRP
                     HLGFANPKEAYKLANYQQEALSFPEPVSWADVERDLSAWLSNDMQRNAIETLYNLINL
                     IRQKGDEKLLEIARKLSTSDHFYYMCTKYFRDGDVHKYFSPYDSPDQAYIYYMNALAD
                     LEERLQ"
     gene            20656..22071
                     /locus_tag="PK015_04505"
     CDS             20656..22071
                     /locus_tag="PK015_04505"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOL33761.1"
                     /translation="MKGKVMVLEDDTVLAEQIALILKNNEYEVQLASNSDTFFAQLRT
                     FKPDVILVDVFLVGSRLNGIQVLKYIKENLDFNYKVIVISGEVTTEQIRQIRELGAYH
                     FLEKGPNFSIEQLLFHIDNAINLKRQEEQNLDLQIEYLNLKRQFTQAFPFIGESATIN
                     KVRNQLIKFAEADEDVLILGENGTGKEVAANFFYINSRRFGKPFHTIFCSALNESIIE
                     RELFGQEKGINGLSDRPYIGLFEKCSEGILFLDEITNLSLNAQAKILRAIENKEIHVV
                     GGSVKKVNTRLILTSNTDIKTFSDPSRFRKDFFYRIEGNIIQIPPLRERGDDIILLMN
                     YFINSYADYYHQSDHLELNSIKDELLTYKWPGNVRELKNFCKYICINEREINNQTILR
                     HLHNKMIQSINETTTGMEKYFYIHNLKHSTAAFERDYILHFLKENNYSVSKTAKVIGL
                     ERTTLYKKIKALGLNNVLREN"
     gene            22075..22785
                     /locus_tag="PK015_04510"
     CDS             22075..22785
                     /locus_tag="PK015_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33762.1"
                     /translation="MKHNLGLKILALAVAIIIWLQIILMDEHQSKVNLELRLVQSTTA
                     DTLRPQPKKILCQVQGKGLDILKLYFSRAYVEMSASDYWAGNDKNFRIVNMPENIKVN
                     FINVIPPPPYENLKAEDLAKVSNKESETTIISDQEKTEELSPPKETTIIDSQDPIVTR
                     ILYELPISSPRNLKIFPPKATLKVQGKASLLARLPQGISITISPTPDNEGYYTVYASV
                     PENVTILDITPQKVRVIK"
     gene            22782..>23668
                     /gene="tsaD"
                     /locus_tag="PK015_04515"
     CDS             22782..>23668
                     /gene="tsaD"
                     /locus_tag="PK015_04515"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOL33763.1"
                     /translation="MNAILAFESSCDDTGVAIVDTDYNILANVVSTQLDHSLFGGVLP
                     ELASRLHLKNIVYLTEAAFKQSKLSPAEISAVAVSINPGLIGSLIVGLSFAKSLAWSW
                     EKPLITVNHMLAHIFANFLEHKSIQPPFLALVVSGGHTELVYFSTLTDFEIVGKTLDD
                     AAGETFDKTAKLLDLGYPGGPIIDSLARKGNPEFFHFPRALNRKNNFNFSYSGLKTAV
                     LEYLSRHKPEYIEEHKADLAASIQQAIIDPLINKTLSWARQNKIKTILLAGGVAANSA
                     LRTQLSEKASKLNIQVFYPS"
BASE COUNT         7618 a         4395 c         3987 g         7668 t
ORIGIN      
        1 ttttattaga gaaaatcaaa caaaccggat ttgatttata tttttatgag ggttataaac
       61 cacataaaga agctttaaat tatctctgtg gtgctgatat taatttagtt atgagagaga
      121 ataatgagga atcccaaaaa gctctatctg gcaaaatctt tgaactatta agagccggaa
      181 gacccattct ttcagttgga cctcaacact cagtaatatc agatatagtt aatgaaactc
      241 aaactggtat tcatgcctat atagaagatc ctcaagctat tgcagaagcc ttacgtatct
      301 gctattctcg tagaaatgac cctccaccac ctaaggaagt tatagaaaaa tattccagag
      361 agtacttaac caaaaaactt ctctcgcttt atccagattc ctgaaaatac atttcgtttc
      421 tctggagata aaaagaatcc ccccagaact tattaattcc ggggggttat cttatatttt
      481 ttggaatatt ctttatttaa tcatcagcaa tttaccacta atcctatctt tatccgcttc
      541 taaacgataa taatagactc cgctatcaac tttcctacct tgattatctg taccatccca
      601 gaaaaggata gcatttccta tggcatcagt tattatatta aattgtttca ctaattgtcc
      661 ttttacattg tagacactca taagggcagg tacattactt ttggtttgaa tagagaaaga
      721 gacatttccc atactgggat taggatatac actaactgaa gtaacagcag gaactgcagg
      781 atcatcaata gcagtaccta ctacgatagt aaccacattg gatggtcctg atagcatcat
      841 aggattgtta aataaagtgc aaacccaata ttgatgagtt ccattaggca ctcccacatc
      901 cgaaaaactt aatatactgg gattagttat ggtagaaatg ggtagttcat ctctaaataa
      961 cctatagcca actagtcctt tcacatcaaa ataaagtgga ggttcccagg ttaattgcac
     1021 agtattgggt ggaatatagg taccatgaag atttcttgga ggtggaagct cctggatagg
     1081 gatagtaaaa gcactcatta cagggatacc attcattccg tgttcatcta caggcacaac
     1141 tctaaagcca agaggtatat tcaggtcttc ttcagtagga gtataagtaa gttgattagc
     1201 tccttcaata gggtggggtt gaccaatcac tctgtaccat tgataaattg aagaaccttc
     1261 tgcatcacca tctatatccg aaaatgtata tgatgcggtt agctgttgat aaataacggg
     1321 aggaccagtt ataaccacat ttgtagcggt tggcgcatgg tttaaaaatc ctgtccctgt
     1381 taagggaata ttaataactg gatgattata aagattactg gtaatcgtta aggttccagt
     1441 ataagttgtt gctgcagtag gaataaaggt cacatcaacc gttatggact gagttccagg
     1501 tatggtgaaa ttggtgatac tactgctaaa gacaaaatta tttatagaaa tagtcccagt
     1561 tacaggttca gcaagaaaat tggttatcgt tataggtaca gtggaacttc caccaataaa
     1621 agtttgtgga aaatctatgt ggtcaaccga tacaggataa gcaccaacta agccaggaag
     1681 agaatatttc acaccctgta aaatctcctt actggtcata ttctgaagga agaataccat
     1741 ttcacaattg gcaatattcc aagaagcatc aggagtaaat gttaaattga tagttgtaga
     1801 ttctccggta tttaaattaa ctggtgtgcc attttggtcg ggaatcatta aacgattcac
     1861 attctctact gtagtttgat tgaaccatac aaatgggata tttgattctg tcaggactgc
     1921 atgcaattta atatttgtat tagtatcatc ctcaggttta gtaactgtaa ctgtaacctg
     1981 gaattgatta ttagattgag ttcccaccgc agaaatagtg taatgtgaag gaacttgcat
     2041 ccgtgcattt accttaggta aatagtttga atacatagag ctactattac ttccacctac
     2101 ggttggattc aacccatcaa agaaagcagt aggaaaacca gatacaccat aaaaactatt
     2161 tcttgcatta gaatagacat tggcaaaact atcgccatta tgatttttaa ttatagctac
     2221 aggatgaccg ttattcaaaa ggtcatggca acccatcgca gcaccagggc aatattggca
     2281 ccaggtgcct gtagcaactt ctactacgac caaatttctt ggtacagcat atagaaaaat
     2341 accagcccat actattaaaa atattgaaag aaagcgtttc attgttctct ccttcctttt
     2401 tgcaaataat tagaaatcaa atttgtttaa atatagaaac atctatttct gtcaagttta
     2461 tcctgcgatt ttcttatgat aaatgtgact gtatactctg gactaggtct aaaagttctt
     2521 ctggctttct caccagataa tctggatgat gacgaagtag attttcttct gagtgaagtc
     2581 cccaagttac actaataatc ttaactttgc tacgatgagc tgccttaata tctctaactt
     2641 catctccaat ataaaaagtg tggttttgat ttaatttatg ttttttaacc tgagtgcgta
     2701 tgctgttgtg tttaccgaga attcctttgg tcccttcaat ccaatcaaaa caatcaagat
     2761 tattacgctt taaaaagtca ttaatatgtt ctgcatcatt agaactaatc aaacctaata
     2821 taattcccca ttcttttaat tttctaatta cagtatccat tccgggacag ggcagtagct
     2881 gcggcataat ttgtctatat tcatttaaga cagtcgttat agcctgaggt agtttggaaa
     2941 gaggaaaatg caattttcgg caggctttgg gtatagatag gtcccttaaa gtattgaaag
     3001 tttcctgaga aagtggctca aaaccataac tgggagcaag cttatttatt aactgaaaaa
     3061 tggcttcaat agaattggca actgttccat caaaatcaaa aattaaagcg aggtccatag
     3121 ctttcatcct tttaatttta gcaccatact atatgcgcta tttgcactta tcctgaatct
     3181 attcgctacc atttgagcta ttgtttttga acttaaacct tgtccaagaa gttcttctat
     3241 atattgattt atttcagtcc tatctatttg ttcagaacgc atattacctt caataacaat
     3301 tacgaattca cccttttctt ttatattata attcgtgata atatcctgaa tattgcctct
     3361 aataaactct tcatatattt tagtaatttc tcgtgcaagg cagattcttc tattgtcaat
     3421 atattcagag agttcagtta aagttctttt tatcctgtga ggtgcttcat ataatataac
     3481 agtgtgtata gagtttttaa tgagagtcag tttttctttt ctatgtttgt tagttagagg
     3541 taaaaatcct aaaaagagaa aactttcagt attaaatcca gatgcggtta aagcaggaat
     3601 gatagcagta gcgccaggta gtgggataat atttatttga tggtcaatag cttcttgaac
     3661 cagtaaagaa gcaggatcgg agattcctgg acttcctgca tctgagacaa cggcaatatc
     3721 cttgccctgt tccaaaagtt caagaatctc cggtatacgc ttcttttcgt tatatttatg
     3781 cagactaata agtctacctg attttatctg ataatgcgag agaagtgaac gcgtattgcg
     3841 cgtgtcttct gcagcaataa tctcaacctt tcgcaggatt tccagtgctc tcagggttat
     3901 atctctcaaa ttcccaatcg gaactggaac aagatagaga ttgcctgctg gcaaagttac
     3961 tccttaaata agatttaatt ctttcccaac ttttttgaaa gctgttatag ctttatctaa
     4021 atcttctcta ttgtgagcag cagaaagttg aacccggatg cgagctttgc ctttaggaac
     4081 tactggataa acaaaaccta taacatagat tccttcatcc agtaacatat ctgccatctt
     4141 cattgctaag ggctcatcat aaagcatcac cgggacaatt gccgtattac cttctacaat
     4201 atcaaaacct gctttggtca tctcttcacg aaaatacttg gcattatccc aaagtctatc
     4261 tctcagttga gaagaaccgg ataacatatc tataactttc aggctggcac caattatagc
     4321 tggagcaaga gagttggaga ataagtaggg acgacttttc tgccgaagag tttctattat
     4381 ttctttacgg ccagttgtga atccaccatt accaccaccc atagctttac ccagagtact
     4441 ggttaaaata tctacccgat cctgaactcc aaaatattca ggagttccac gaccgttggg
     4501 tccaatataa cctgtggcat gcgaatcatc aaccattact aaggcatcat atttatctgc
     4561 caaatcacaa atttccggca actttatcat atcaccatcc atagaaaaca cgccatcaga
     4621 gcaaattaaa cgatatttca tattctgagt ttgtttcaga atgttctcaa gttcttccat
     4681 attggaatgt ttataacgaa atcgttgagc tttgcaaaga cggacaccat ctataataga
     4741 tgcgtgatta agttcatccg aaataatagc acattcttca tccagaatcg gttcaaaaag
     4801 accaccattg gcatcaaaac aagctgcgta taaaatagtg tcttcagtgc ctaaaaattc
     4861 agatatcttc ttctctaaat ccttatgaat ttgctgagtt ccacaaataa accgaacaga
     4921 tgccaaacca taaccccaat cattcataat atcctgagct gccttgataa cctcaggatt
     4981 attggctaat ccaagatagt tattggcaca gaaattcaag acagtttgac cggtgctgac
     5041 ccgaatggaa gcaccctgag gtgtggttaa tatccgttca ttcttataga gacccttttc
     5101 ttttagttcc tccagcatat tttgtaaatc tgttttcatt ttgccaaaca tggcaaacct
     5161 ccatggttta gttttaattt tatattacaa tttttgaaat tcactattca gtcaataaaa
     5221 aagctaaaac atatcggact caaacgagct attattgaat tatctataga ttagatacaa
     5281 tttgccctct cttagtaacc tatgggtaat ctctacttag ataactaata aggattgaga
     5341 ttttcttttg agttagaaaa aaaagctccc caaatctgga gagctttaag tatggtgcag
     5401 aaggcgggac tcgaacccgc acacccatta caggcacaag atccttagtc ttgcgtgtct
     5461 gccaagttcc accacttctg cataaattta tttctttaaa tcttctgttg ttttctgagt
     5521 tgaagaagaa ccatcacttg ctttggtctc agtctgttca ttattacctg gtattgtggt
     5581 cactgactta tttgtctttg attctgtacc agctggggct ttggaggttt caccaaattg
     5641 ttcttctaat atcttaggag cttttgcctg atttgtcttt gtcacttgaa aagccagaaa
     5701 aagtgatgaa atgacaaata agacacctaa aatctgagtc catttcctta aaaatttaga
     5761 agctccacta cctccaaata cattcattgc tgctccacct aaattggcat ccagaccacc
     5821 tttggatgtt tgagctaaaa tcacaaatac cagtgcaaca cagattatga tatgaatgac
     5881 caaagataca gtatataaga ccattctttt ttccttttct atttaaaaaa tcctaatttt
     5941 tttccttatt ttatttcctc catttttgtc aatcataaaa tactatttaa ttcctactac
     6001 atttgaaatt tcagtgagtt aaaaaaatta tccatttaac agagtaataa aataagagtt
     6061 ttatatctta taaggagata tgtattttat ttttttgttt tagcgcactg aaaaataact
     6121 ataacctaaa tatttttaat ctatttggaa atccttctaa aaattcacca taaaattatt
     6181 gacaatgttt ctaaagaaaa ttgtaatgaa aaaatatgag gtatttatgg aattcatttt
     6241 acctgatgga aaaattttag aactggagca agtccgtaag gtctccagga ttcgtgattt
     6301 gggtttagaa aaggactcta ttgaatatag taagatagct tttgagattc atttaaaggg
     6361 tcataaaata atagaggtag gtgaacgcta tcattatgct gattgggctg aaaaattgaa
     6421 gaaattaacc acgattagaa ataatttgat taacgctcta aaagaggcag gaatacaatt
     6481 tgaagaggag taaaaaactt aattattagt gttaataaga aggaagggca ggaaaaccat
     6541 tttcctctat tttttagcta aagaaacgat tttatctact aattctggaa agtttattcc
     6601 aactgctttt gctgccatag gagtaaggct taaatctgtc attcctggaa gagtattaac
     6661 ttccagaaaa taaggtttat ttccatcata gcgaaaatca attctagcat atccttttaa
     6721 ccccaaaacc ttccataatc tttcggcata aagttgaata agctgagaaa ctgattcttc
     6781 tactggtgct ggacaaagat attcagaatg acctttactg tatttattat taaagtcata
     6841 ccagccattt aaaggtctaa tttctattaa gggtaaggct ttcccatcaa gaactgttgc
     6901 ggttaattct ttcccgggaa tgtattgctc taaaaggacg ctatcagaat aagttaaagc
     6961 aagttttacg gcttccttca actcaaataa tttttctact atacttatac ccacggaaga
     7021 accactgtca tttggtttaa ctattatagg cagtccaagt tttgcctcta tagcggaaaa
     7081 atcatttgga tcctgataat catttagtaa atcttccctc ataagtatat attcaggagc
     7141 cggaatacct tcatctaaag ccataagctt tgaaacatat ttatccatgg tgagggtgca
     7201 agctttagga ccagaaccgg tatatttaaa acctgcaaga gaaagagccg cttgtaattc
     7261 accattttca cctgtaccac catgaagacc aataaataca atggaaatct tctctgttct
     7321 aagtttgctg attaaggaat aaaaatcaga ataatcagct ggatcaagtt ctataacctc
     7381 atatcctttt tctctcaagg cttttgcaat ggcttcacca cttctcaaag agatttctct
     7441 ttcgggagaa attccacctt ctaaaactgc tattttttcc ataatttctc tcacttttct
     7501 attataaaaa acaactctaa tttaaatccc attgccgctt taaatcgtgc gacatcagga
     7561 ttatttccac cacaaaaatc tatcttatca aatcttcccc ttagactttc tatgaaagtt
     7621 agataatgaa caacgctggc accatttttc atctcttcag gaagagtgct taacataatt
     7681 agatatgctc tatcatcttc ttttccaccc aatactaagc ttgaacttac aacttttccc
     7741 tctcttctaa ggttgaattg agaaagtatt tgatgctgat ataaatcctc catccatttt
     7801 tgaaatgcac gataagataa ccctaaatct ttatttagcc gttgatataa tatcttgagc
     7861 aaatggataa actcttctgg aaggaattgc tcatccaact gataattata aatggacgct
     7921 ttgcttaatt tcgttttctc atcatataag agttttagtg gttcctgaaa atcataggta
     7981 aaggtataga gcggttttgc tttataattt ttccaagtga aacctctaac atcatagttt
     8041 tctggcatta gtttgaattt catcttttta taatgagctt tcaaccatat tgctatgtcg
     8101 gaactgatat taagttcatc cagcagtcta cgattttctc ccttttttcc taagttgtaa
     8161 aaccatagtc cttgataata agaacttgta ggacaaataa gataactgat tcccaatttt
     8221 ttcttttcat agaggggcat aactgccttt atctcatcac ctttataaca gacaagttgt
     8281 ttggcttcac agttatgaag attttggact gctaacataa aatccggatt tatccataga
     8341 ggacaaaagg acagaccttc tttacttgga aaacgtgcta caatattata tttggactta
     8401 ctcatattgc attatccttt attcaaaaaa gcagatataa ggtaaagaaa acttagagta
     8461 cgacggttaa ttaaattttc tctcaaagaa taaaactcct ggataaatag gaatggatta
     8521 ttcaattttc cccatcctat ttttagtcta taacttaatg aaaatcaata taatataatc
     8581 tatttaatca ttttagagaa cttgctttaa tcgtcaataa aaaaactgtt aataaatgta
     8641 tttcatcttt tgtattgacc aaaatacgct tatattatct atggatatat tataataaat
     8701 gaggtcctta tgagaggaaa agttaaatgg tttaacaaga ataaaggtta tggttttata
     8761 atcacggatg ataataaaga atactttgta cattggaaat ctatagtaac aaattcacct
     8821 cgggaactta aagttctgga acaagatgaa gaagttacct ttgatttaat ggaaactgat
     8881 aaaggcactc aagccattaa tatcattcgt gttaattcct aagccaaaat tctgttagca
     8941 taattctcag gggcaaaaga gtatatcttc tgcccctgaa ttttatccta tgcctttttt
     9001 acctatcaat ctaaaagaag ccagagctcg tggatgggat gagctggata ttataattgt
     9061 taccggtgat gcctacattg accacccaag ttttggagca gctataattg gaagagtcct
     9121 ggaaaaagct ggttttcgcg tgggaattct accacaacct aatcctgaaa aagatgagga
     9181 gtttctggct cttggtattc cccatctctt tttcggagtt actgctggca atatggattc
     9241 tatggtaagc aattacactg ctcaaagaaa aaagagaaac aatgatgcct atagtcctga
     9301 cggcatttct gggttgagac cagaccgggc aagtttagtc tatactaata ttcttaaaag
     9361 aatagctaag aacactccta tcgttttggg tggcatagaa gcttctttaa ggcgaatagc
     9421 tcattacgat ttctggcaaa ataaaatacg tgccagtctt ttagctgata ctaaagctga
     9481 tattctggtt tatggtatgg ctgaaagagc tattcttgcc attgctaaag cattacaaga
     9541 aggaaaaagg atttctgaac ttaccgatat tgcaggaact actgttttta gtaagcaacc
     9601 tcctgaggct gatgaaatta ttcttccaga taatatcgct tgtcaggata aattcacctt
     9661 tcatcagatg acccatattt ttgaagagaa ttatttatcc agaaccatct atcaattaaa
     9721 tggcaatcgc tggataaaac ataaccctcc agcagaatct ttaactcaaa aagagatgga
     9781 tgaattatat ttacttcctt ttgaatatgc tccccatccc atttataaag aacacaagat
     9841 tccagcttat gaacagataa aggattcttt aactgctcat agaggttgtt atggaggctg
     9901 tagtttttgt gccattgcag tacatcaagg aagaaaaata agctggcgtt cttctgattc
     9961 tctggttcaa gaaataaaga atattactgt gaaaagaaag cataagatga ctattactga
    10021 tgtgggaggt ccaagtgcta atatgtatgc ttccttttgt tctctggatt ttccttcttt
    10081 ttgcaagcgc tcttcttgca tctatccatc aatctgtcta aatttaaatc ttgctcaaga
    10141 agaacaatta aaagtacttt ctattcttga aaacctacca caagttaaat ctgtatttat
    10201 ctcttcaggc atcagacacg atcttgctat tctttctcct caatatataa aagctttaac
    10261 cctaaaatat accagtggaa gattgaaact tgcaccagaa cattgtgtcc ccaaaatctt
    10321 gagattaatg caaaaacccg atacttcttc ctttgagaag ttttctcaga ttttcttttc
    10381 cacctgtgaa gaagcaggaa taaaaagaca aattgtcccc tatctcattg ttggacatcc
    10441 aggttccacc attcaggatg cttataaatt gcgggattgg ctaaaaaagc ataatttaaa
    10501 agtggaacag gtgcaagaat ttactcctac tcctatgaca ttaagcacct gtatgtatta
    10561 tactggctta aattattgga ctggtaaacc ggtgtatatc cctaaaccgg gtgaaatcag
    10621 aaagcaaaaa aatatcattc ttagcttatt taaataattt atatcataaa tttatataat
    10681 cccaatttat ttttctatac tttctctaac ttattgcaaa ttaatatgat tacgaaccct
    10741 ataatctctt tcagccataa gtatgtgttg ctttaaccaa aataagaatt attattttac
    10801 ttgacaataa tatcattgaa gatagttgga tttatatgag gtaatgaatg aagaaaaatt
    10861 tgctgctaac ggtcgggttt atctttttct gcataatggc aggtgctact tctatataca
    10921 atattcaata taccaatagc cgtggtgcag ataatagtta cccctcacct tatctgggta
    10981 aacaggttac cgtggaagga attgtgactg ctgttaattt caatggagaa ggttatttcc
    11041 tttctgaacc agttagtgga gcctggaggg gaatttatgt attggataca gagcataaac
    11101 ctaaaagagg aaattacttg caaatatccg gggaagtagc agaatacttt ggaatgacct
    11161 gtattcgcaa tttgaactca taccgggtct tggaccaaca tcgcagttta cctactcccg
    11221 taattatcag taccaaccag ttaactaatc ccttagaagc tgaagcttat gagggagtat
    11281 atgtccgtct tgtaaatgta tctgtctcca gtatcaaaag taaaaatgga aagtttatgg
    11341 ttaatgatgg aagtggcccc tgtagtgtgt cactgggaaa ttttgggact aaaacaaatt
    11401 tacccgcttt aggaacacaa tttcttcaaa tatctggtat tgtaactttt tcttatggag
    11461 agtatactct caatcctgta agttctgatg acctgcaaat tcaacaacct gtttacattc
    11521 agactcgtag ctggggctct atcaaatcat tatataaata aaatttaagg agtataaaaa
    11581 atgaaaaaat tctttaagaa tacacttatc gtcgcaggtg tagcgctggc tgctcatttt
    11641 ggcgttaaag cttataaacg tattaatgga gttataaagc tttcaaaatc gctcggtgat
    11701 ttcttgaata atgtctatgg agttaagcct aaaataagta ttaatagcgg aataaatact
    11761 ataaatgcca acaatatcaa agtagaattc ccgcaagaga tcctggataa atacactgat
    11821 attgaaacta ctatccgtga gtatattgat gatttttatc ctgaactcgc taaaaccaaa
    11881 atcaatattg aaatccaacc tttaagcgaa caggaaggcg agaacattaa tgtagaagtg
    11941 gaacctgaac cagctgacta aaaaattaat aattcaaaaa tcctaatttt tgcacccgtg
    12001 tctttttgat gcgggttttt ttgtggattg agtttcatta ttttatagac atcaatatat
    12061 gatgatagaa tataattata tatcctaatg tgtaaaaagg taaatgagag ataactactt
    12121 tatttagagt tatttatcct tgattattgt ccttcttgac ctatctgttt atttttaagg
    12181 caattacagc ttctccctta gctctccctt ggctctttct tagctcttta gctaagtgaa
    12241 agataggagc gagctaacat agcaatttaa tctttataat tatctgataa tattaacttt
    12301 aataaatcat ctctatcata aaaaagaagt ttggataatc caaacttctt gatttaatat
    12361 tattttactt tatttaaaat tatctaattt ctatgcccca ggttttagaa atagagatcc
    12421 cgcacccctt tttccatctg ctccaatttg tttttaactg cttgtttcat attttcatct
    12481 tcaatttgtt ttagttctcg gtctattaac tcaaaaccaa tttgccttgt ccgctccgag
    12541 gcataatcat aaagatattc agcaaaagtc agtaaagcat taggagtgca aaaccttttt
    12601 acaaatccgg gaatggcaaa ttccataaaa tgttcacctg tcctaccagc acgataacaa
    12661 ctggtgcaaa aggaaggaat ataaccctct tgcgccagtt catatattac atcatccaga
    12721 ctgcgtgtat cccctaaaac gaactgactt ttctttcttg attcctcatc tttggcactg
    12781 taatcgccta ctcctatgct ggatccggca tctatctggg aaataccata acgaattgcc
    12841 tcattacgaa gttctgcttc ttctctcgca gttaaaatca tacccgtgta aggaacactc
    12901 aatcggataa tagctattaa ctgcttaaaa tcttcatcgg gaaccttgtg aggtggattt
    12961 tccgtaaatt ctgtaccaat agctggttct aaacgaggaa aagagatagt atgtggtccc
    13021 actccaaagg tttcttccag atgaatagtg tgataaagca gtcccatcac ttcaaacttc
    13081 cagttataca aacccattaa agcaccgata ccaaggtcat ctattcccgc tttcatcgcc
    13141 cgatccagtc catataaacg ccaaagaaaa ttgcttttag gacctctcgg atgcaatgtt
    13201 ttataagtct cttgatgata agtctcctga aatatctgat aagtaccgat tcccacagat
    13261 ttcacaatcc tgtaaccttc tatatccaga ggagctgcat tgatattcac tctacgaatc
    13321 tcaccctttc caaattttgt atcatataca gtttgaacag tttgagcgat aaaatcagga
    13381 gagtattttg gatgctctcc ataaaccata atcaatctct tatgcccttt tgattccaga
    13441 gcttgcgtct cctgcactaa ttgttcgtga cttaatgtgg ctctaataca ttctttattg
    13501 gaacttctga aaccgcaata aacacaatca ttaacacatt catttcccac ataaagcggt
    13561 gcaaaaagca ctatccgatt tccataaatg gtctttttaa gctcatgcgc tccttctctg
    13621 attaattgtt tcaattcagg gtcgctcaca ttaagcagag ctgctgtctc ttctggtgca
    13681 agcctgttct tctccaggct tttagcaata atctctctta tttgagattc ggaaggattc
    13741 tgctcgcttt gaagcaactc ctcaatcttt tccgttggta taaaggactc cagtccttca
    13801 gtatctatat tcattttaac tcctttttat tatactttta aagtcatagt ctttacttta
    13861 acggactcaa tctgacccaa ttttcctgca aaagaaccca ggatatccag attggcttcc
    13921 ataatcaaaa aaatgacgga cacatttcta tcccgcatag gataacccac ccttaaaaga
    13981 atataggaag catgttgatg aataagttcg gaaacgggat tgaatgcctt ctccctatct
    14041 tctattacaa tagtgacaat gtgcctcttt tcatccatat aactatcctt taaaattcat
    14101 ttgcctggga tgaaggagga ttgtaaaagg agttttgctc ttttcttacc acccttgatg
    14161 ccggaactta tccctcatct caggtcttta tttgatataa tatttatctt accctttctg
    14221 gcaagatatt tcttaagaaa atcaatactg cgctgattct atatcctata aaaactatca
    14281 ctggaaatgt aatcaatttt gacaaacaaa atctgctcct gcataaataa cattctcttt
    14341 aacctcaaat tccagttcag gaagcataat cagacgatag atgttttgat attttttctg
    14401 tagatttaaa taggttattt tctggtctaa ttctctaagt aacatagctg gtttaatgtt
    14461 tttcccccat tttgcttcac caataagtag attttgaccg tcaacagact cagttataat
    14521 atcaatttca attggtttat cagttttgga ctgttgccac catctttgag caggaagaaa
    14581 ttgtatgttt ttaacattta aattaattac actttgtcta catagttctt cccaggtttc
    14641 tgaaacatag acttgccatc ttttttttat ttccttctct accacatcaa taagccgact
    14701 gtttaaaatg gattgattgg gaacaacaaa ggtaaagaaa aatctaataa gagggtcatc
    14761 taatctataa agtgtttttt tattattctt gaaattttca ccccagcaca attccctttt
    14821 tacataacct gcatcaatta agacttgcaa tgacctacta aggctggtgg ctggtttatt
    14881 taatctacca gcaattactg aaagtttatt acaacccagt cctattaatg aagctatgct
    14941 aattggctga actgccccac ttaaatcatc cataaaaagt ctcaaaggct cttcatataa
    15001 aataccatag ggattaagaa tagcttctct tatcgcttca aataaactat tatattcagt
    15061 tctaacctcc caataccttg gtatcccacc ccagaccgaa tattcctcaa ttgcttgaat
    15121 ggatgttaga ttcagtattt gtttcaacca cttgcatttt aggggctgaa gtttaataat
    15181 tctatttgct cgtccaaaaa gaggggaact tttttccaga aatgtgctat acatcatctg
    15241 ctgtaaacta ccacaaataa ttaaatgaaa attcaaattc tgctttaaat caataatctt
    15301 ttgaagtaca cttggtaatt ctggtgcact tttcactaaa ttaggaaatt catcaataat
    15361 aatagtagcc tttttagata gttgcctatt tgcctgttca aaaaaggtat accaatccgg
    15421 gtacaaaaca ttattaaacc ctggaattaa tttcgctacc tgaacagcaa aaaaattccg
    15481 ctgaatatcg gaagtattaa tatcagcaag ataatagata caatttgtag gtttaagttt
    15541 ttgcaataag gcacttttgc caacccttct tcttccatat aatacaatta attgactttc
    15601 ttttttactt aattcctgtt taattaagcg taattcttct tctctatcta caaattccat
    15661 cttaaaaaac ctccttactt cgttatgttt ccattatttt aaattcatta tgtttgtcaa
    15721 acattatgtt tatcaaacat aatattccta agaataatgt tctattctcc tataatttac
    15781 ttattctctt ttcattcatt agtaagctat attagttcat ataaattata cacctaagag
    15841 aatgcgatat ttcactaata actttaataa catctattta taaaagtaaa atcatctaac
    15901 tctctaaaat tgaaatgatt gataaatcac ttgacggaaa gagggttatg gaaaaaattc
    15961 cacataataa cccccaaatc caaaaaaagc atatagtatg aggaggtaaa ttgagtaatt
    16021 attttatgga gactcaccac catgaatgga ttttgaccaa taagcttggt ggttatgctt
    16081 taggaacagg aaatctgata aatcagcgta aatatcatgg gcttttgata gccagtgaca
    16141 aagattttca acgctatcat ctggttgcag gaatggaaga aaaagttgaa tggcgtggtc
    16201 aaactctgta tttagatagt aataattata gcaattgtat ctatcccgaa ggattcttat
    16261 gcctggtcaa accctggcta cgtccttatc ctatatttct ttattccgct ttacctcatc
    16321 aaaatgatat ccttatactc aaagaactta tgatggatga agatagcaac actatcctgg
    16381 taaaatacac caatttggga catcataaac tgcatctcac tttgctccca aaatatacta
    16441 tgaccccaca tcacgaactt aatgctccag gaagtctgga tagggaagtc tttgatacag
    16501 aattcaataa tatagatgat aaatgtttct ttaatgtagt ccgtcccagc aatggtttaa
    16561 gggtttttgg atggatggat aaaggagagg ttgattataa aagattaact tactacaatg
    16621 ttttttaccc ctgggaagtt atgagtggtt atgcaggtat aggagatcag atagcatttt
    16681 ttgaattgaa cttttcttta gctataggag aaagccgatt cctgcttttt tctgaccagc
    16741 caatcaaaaa tccagaaaag ctgattaatt caattgaaaa gcgttattct gctcttccta
    16801 aacctaaaga ttatcctacc gttccagata aagatgatac tcttatcagc aaacttgatt
    16861 ttgaagataa tttccttttc tcttatgagg aatatataaa gattctggaa tttgctctga
    16921 aagattttat atccaataat gatattgttt ccggttatcc tttttatggt ccctggggta
    16981 gagatacaat gattgttctg aataccctcc ttcatatgcc caattatctt gatttagtag
    17041 aaaagatact gaataaatat aagaaccaaa tgcaagatgg tttaatccct aatatgctca
    17101 aggaatcagg aagaaatacc aattacgata cggttgatgc cactctctgg tatgttatcc
    17161 tactttggaa actgggaaaa tacaaacagg atatagcttt ctggaagaaa agtattcggg
    17221 ttatagaaga agtgctggac tatatcctaa ccaatactaa atatcccttc tttatcagag
    17281 aagatggatt aatagagctc aaggaagaat ttgctcatgc cacctggatg gatgttcgca
    17341 ttgatggaag agcagtaaca cctcggaatg gcgcacccgt agaaattaat gcactctggt
    17401 ataatgctat ctgttgctat gcatctatgt gtgaagaata tactaaatta tctggcatat
    17461 cctatccacc acaggaaaat gtgataaagc ttaaagacct ggtatacgag tctttccaaa
    17521 aattctggat aaatggttat ttggcagaca gattattagg agatgaaccc gtagaagaaa
    17581 ttagacccaa cgctgttatt gccttaagtc ttccctggca attgatagat aaagaaaaga
    17641 tggaacaagt ttggcaagtt gcctataacg aactttacac accttatgga ctgcgcactt
    17701 taaatccaaa gaatttccgt ttccgtaaaa aatattatgg aactcaaaga gaacgagacc
    17761 ttgctttcca taatggcagc gtttgggcat ggcttttagg tgctttctgt ggtctctatc
    17821 ttaaaattca tagagacacg atgtctgatc aagaattagc tgaaaaactg ggagaattca
    17881 tttccacttt ccgttatagc tttatgagag gtcatattgc cactattgca gaagtctggg
    17941 atggtgatca cccccatttt cccaaaggtg caccagcaaa agctatcagt gtggctgcat
    18001 tatataatat agaatcctat atagctactt taaaaggtat catcactcct cttcaaaaga
    18061 aaccagtaac cagaaagaaa acattgcaaa ggaaaagaaa atgaaagtct taatgttcac
    18121 ctgggaattt ccccctttaa ttgctggcgg tttaggaatg gcttgctatg gaatggttcg
    18181 tgcccttctt ggtcttggta taaaagttga tcttgtcctt cctactaaag aattagtgta
    18241 ttttccttta agagagccag acgatgttga caaacttcca gctgtattcc tggaccccaa
    18301 aaagcataca gaatatatca gtcataaatt cctaaatata gaagaacgtc tggattatat
    18361 tggcgtaact gtccatcctg aaacctatct tcaattgggt gaagtccaaa aatttgttaa
    18421 agccatcaaa aaagaatatc aaagagaaag cataactcag gaagaaagag aaatctggga
    18481 agaacttact gctcatctaa tgggtgatga agatatcttt aaaaaagtgc aagagtatac
    18541 tatacgtgcc gaaagatttg cgcgagaact ggattttgac ctcatacatg ctcatgattg
    18601 gttaacttat ccagcaggaa tgcttgctcg tagcatttct ggtaaaccat tagtggtgca
    18661 tattcatgcc acagaatatg atcgagctgg aggtcctggt aatcccagag ttcatagaat
    18721 tgaaagagca ggaatgatgt ttgccgatag agttattgcc gtctcacaat atactgctca
    18781 gatggtgatg tctcattata gaatagatac ggctaaaatc agtattgtgc acaatgcttt
    18841 ttctgtctct gatgaatatc atagaaccaa aaaacgcatt ttcaaaggac cgactatcct
    18901 ttttctcgga aggataaccc ttcaaaaagg tccagattat ttcttggaaa tggcaagtaa
    18961 agtccataaa gtccaccctg aagctcattt tattatggca ggaacaggag atatggcacg
    19021 agaactactc tataaatcag ctgctaagaa attgggaaat aaatttctct ttactggttt
    19081 tttgaatagg caacaggtgg aagatatcct tcgcgcttct gatatctttg tgttgccttc
    19141 tgtttccgaa ccttttggaa tttcacctct ggaagcaatg gcttttggag tgacggcaat
    19201 tatctccaaa caatctggag tgtcagaagt ggttaatcat gcctttaaaa tagattactg
    19261 ggatgtagat ttaatggcag aaacagttaa tcatctgatt gaaaatccag atctttgccg
    19321 caaaatcggt ttagaaggta tggaagaagt taataaaatc cactggaatg attcagctga
    19381 aaagattcgt caaatctatt cttccactct ttctgaatat ttaaaaagta atccctaata
    19441 ggtgataaaa tgttatatgt agtattttac tttcaagtgc atcaacccta tcgtttgcgt
    19501 catttaaatg tattagatat tggtaaaggt gtagattatt ttgatgaaca tctgaatagt
    19561 cagattatgc gtaaagtcac tgaaaagtgc taccttccta ctaataaact gctacttgat
    19621 ttaattcata gatatgaagg tcaatttaaa gtagcctttt ctttaaccgg aacagctatt
    19681 gaacagatga aagcttatag tcctgaagcc ctggattcat ttaaggaatt ggtaaataca
    19741 ggttatgtgg aactattagg agagacttac tatcattctc ttgctttctt gtttgatacc
    19801 aatgagttct tagatcaagt ggctatgcac cgagatttaa tgaaaagtga gttcggttat
    19861 taccctgaaa ccttccgtaa tactgagctt atctatcagg acagcctttc agaccttatc
    19921 tatgaaatag aaggatttca taccattctc acggaaggtg tagatagaat tctgcaatgg
    19981 cgttctccac tttatgccta taaaaactat gccaagacta tcaacctgct tttaaagtat
    20041 tatcaattgg cagatgacat cgcctttcgt ttttccgata gagattggcc cgaatatcct
    20101 ttaactgtag ataaatttgt gaactggatt gatcgtctca ctttatccga aaaagaaggt
    20161 aggaatcttt tcttaaatct ctttatggat tatgaaacct ttggagaaca tcaatgggct
    20221 gaaactggta tctttgattt tatgcagcac ttccctgaag aaattctcaa gagaccccat
    20281 ctgggttttg ctaatcctaa agaagcatat aaattggcta attatcaaca ggaagctctt
    20341 tcttttccag aacctgtttc ctgggctgat gtggaacgag acctttctgc ttggctctct
    20401 aatgatatgc aacgcaatgc cattgagact ttgtacaatc tgattaatct tatccgtcaa
    20461 aagggagatg aaaaactctt ggaaatcgct cggaaattga gcacttcaga ccatttctat
    20521 tatatgtgca ctaaatattt ccgggatgga gatgtgcaca aatatttctc tccctatgat
    20581 tcacccgatc aagcttatat ctactatatg aatgctctgg cagatttaga agaaaggctg
    20641 caataaggtg ctaaaatgaa aggaaaagtg atggtattgg aggatgatac tgttcttgct
    20701 gaacagattg cacttatctt aaaaaataac gaatatgaag tgcaattagc ttctaactct
    20761 gatacctttt ttgctcaact aagaaccttc aaaccagatg taatcttagt agatgttttt
    20821 ctggttggaa gtcgccttaa tggaatacag gtgctgaaat acattaaaga aaatctggat
    20881 ttcaactata aggtgatagt tatttcagga gaagtcacta cagagcaaat tcgtcagata
    20941 agggaattgg gtgcttatca cttcctggaa aaaggtccta atttcagcat tgagcaactt
    21001 ctatttcata ttgataatgc tattaatcta aaaagacaag aagaacagaa tttagacctc
    21061 cagatagaat acctaaatct caagagacaa ttcactcagg cttttccctt tatcggtgaa
    21121 agtgcaacca tcaataaagt tcgcaatcaa ttaattaagt ttgctgaagc agatgaagat
    21181 gtgctcatcc tgggagaaaa tggaactgga aaggaagtgg cagcaaactt tttctatatc
    21241 aattcccgtc gttttggtaa acctttccat accattttct gtagtgcact caatgaatcc
    21301 attattgaaa gagaactctt tggacaggaa aaaggaatta acggtctctc agatagaccc
    21361 tatataggac tttttgaaaa atgttcagaa ggaattctct ttttagacga aatcactaac
    21421 ctttctctga acgctcaagc taaaatttta cgagccattg aaaataaaga aattcatgtc
    21481 gttggtggtt cagttaaaaa agttaatact cgtctcatct taacttcaaa tacagatata
    21541 aaaacctttt ccgatccttc tcgttttcgt aaagacttct tctatcgcat tgaaggaaat
    21601 ataatccaga tcccaccatt aagagaacga ggcgatgata taatactttt aatgaattat
    21661 tttatcaata gctatgccga ctattatcat caaagtgacc atctggaatt aaatagcata
    21721 aaagatgaac tccttaccta taaatggccc ggaaatgtac gagaattgaa gaacttttgt
    21781 aaatatatct gtattaatga acgggaaatt aataatcaaa ccatactccg tcatctacat
    21841 aacaagatga ttcagtctat aaacgagact actactggaa tggaaaagta tttttatatc
    21901 cataatctta aacatagcac tgctgcattt gaacgggatt atatcctgca tttcttgaaa
    21961 gagaacaatt atagtgtaag caaaactgct aaagttatag gacttgaaag aactactctc
    22021 tataaaaaga ttaaagctct cggcttgaac aatgttttac gagaaaattg agctatgaaa
    22081 cataatcttg gactgaaaat actcgctctg gctgttgcta tcattatctg gctgcagatt
    22141 atccttatgg atgaacatca aagcaaggtc aatcttgagc tcagacttgt tcaatccact
    22201 actgcagata ctttaagacc ccaacccaaa aagattctct gtcaggttca aggaaagggt
    22261 ctggatatct taaaacttta tttttcccgt gcttatgtag aaatgagtgc ctctgattat
    22321 tgggcaggaa atgataaaaa cttccgtatc gtgaatatgc ccgagaacat taaagttaat
    22381 tttataaatg ttattccacc tcctccgtat gaaaacttaa aagccgaaga tttagctaaa
    22441 gtatcaaaca aggaaagtga aaccactatt ataagtgacc aagaaaaaac tgaggaatta
    22501 agtccaccaa aagaaacaac cattatagat agtcaggacc ctatagttac cagaatttta
    22561 tatgaattac caatatcatc ccctcggaat ttgaaaattt tcccacctaa agctacccta
    22621 aaagttcaag gaaaagcttc tttacttgcg cgtttaccgc aagggatatc cataactatt
    22681 tctcctactc ctgataatga aggatattac actgtttatg cctcagttcc tgaaaatgtg
    22741 actattctgg atataacacc tcaaaaagtc cgtgttatta aatgaatgcc attctcgctt
    22801 ttgaatcctc ttgtgatgac accggagtag caatagttga taccgattat aatattttag
    22861 ctaatgtagt ctccactcag ttagaccatt ctttatttgg tggtgttctt cccgaacttg
    22921 cttcacgtct tcatctaaaa aatattgtgt atctcacgga agctgccttt aaacagagta
    22981 aactttcccc tgctgaaatt tctgctgttg ccgtatccat caatcctgga ttaatagggt
    23041 ctttaatagt tggactttcc tttgctaaaa gtcttgcctg gagctgggag aaacctttaa
    23101 tcacggttaa tcatatgctg gctcatatct ttgcaaattt tttagagcat aaatctattc
    23161 aaccaccttt tttggcttta gtggtatctg gtggacatac tgaattggtc tatttttcca
    23221 ctttaaccga ttttgagatt gtaggaaaaa ctctggatga tgccgcaggt gaaacttttg
    23281 ataaaacggc taaacttctt gatttgggct atcctggagg tcctatcata gattctttag
    23341 ccagaaaggg aaatcccgag ttctttcatt tcccacgtgc tctaaatcgt aaaaataact
    23401 ttaatttcag ttatagtggt ctgaaaactg cagttctgga atatctaagt cgtcataaac
    23461 cagaatatat agaagaacat aaagctgatt tagctgcttc aatccagcaa gctatcatag
    23521 atcctctcat caataaaacc cttagctggg caagacaaaa caaaattaag accatattat
    23581 tagctggagg agttgccgct aattctgctt taagaacaca gttaagtgag aaagcttcca
    23641 aattaaatat ccaggtcttt tacccttc
//
LOCUS       DAORZF010000027        22778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_1907_length_22778_cov_339.705849, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000027 DAORZF010000000
VERSION     DAORZF010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 22778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22778
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1907_length_22778_cov_339.705849"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            715..1791
                     /gene="buk"
                     /locus_tag="PK015_04520"
     CDS             715..1791
                     /gene="buk"
                     /locus_tag="PK015_04520"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOL33764.1"
                     /translation="MSYRILAINPGSTSTKIAVYEDDQPILEKTLRHSPEELAPFPDI
                     IDQYEFRKKIVLEALAEGNIAPETLSAVVGRGGLIKPVPGGTFKINEAMKRDLRNPNL
                     WGRVHASNLGAFIADSIAKEYNIPGFIVDPVVVDEFEDIARISGIPEIKRNSLFHALN
                     IRYISRLLARELNQTLEESNFIGVHMGGGISVAAIKNGRVVDVNNALLGDGPFSPQRA
                     GTLPIGDLVELAFSGKYSHSELVKYLSKNAGWMAYLGTDNGIEVYNRIQNGDAYAKLI
                     TDAMCYKVAKEIGGCAAVLEGKVDAIFFSGGLSFNEYIVEQIKSRIQFLAPIYLYPGE
                     KEMEALAQGGLRVLRGLEEAQEYS"
     gene            2130..2897
                     /locus_tag="PK015_04525"
     CDS             2130..2897
                     /locus_tag="PK015_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOL33765.1"
                     /translation="MNTEPSFLSKNRIKELARLKQKDNRLQEKLVVVDGFRTLEQIKF
                     YGINPLEQYLLEGMQPIPIWDNIPVYYISEWDMHKICDSEHPSGFAALFPIPQAQKVN
                     FNCAFYLDRIGDPGNLGSIFRIAAAFSIDTLLLSPDCVEVSSPKVIRSSSGSVYKVPF
                     RQANISELKALKGRIILADAHKGQALTKFNFQPEEKLIIVLGSEAHGISSEVSSLTNE
                     ALHIEMSSAIESLNVSISAAIIAYHLFIQRNTNNLTK"
     gene            2948..3511
                     /locus_tag="PK015_04530"
     CDS             2948..3511
                     /locus_tag="PK015_04530"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOL33766.1"
                     /translation="MNIINNNKTGWIEVICGSMFSGKTEELIRRIRRAEYAKQKVMVF
                     KASIDNRYDATDIVSHSQMRAPSLPIVKPEEIYQYLTDDITIVAIDEAQFFDETIVDV
                     CNDLADKGYRVIVAGLDQDYRGKPFNSMPQLMAIAEYVTKVLAICVKCGNPANRTQRT
                     VHQDDQILVGSSEAYEPRCRNCHKVLD"
     gene            3511..3777
                     /locus_tag="PK015_04535"
     CDS             3511..3777
                     /locus_tag="PK015_04535"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HOL33767.1"
                     /translation="MMGRTILGDIAGFIASLLDIYILIILIRALMSWFMLYHQSKFYY
                     FLVRITEPVLAPIRRILPRMRVDFSPVIAIILIQIIISILNLIK"
     gene            3797..4375
                     /locus_tag="PK015_04540"
     CDS             3797..4375
                     /locus_tag="PK015_04540"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HOL33768.1"
                     /translation="MTLFPTDIRHQEFSTTMFGYSKKEVKEFLEQLANELEDYQRRQE
                     KELQRREQMQVEVQQEAIQASSAVEELKRREELISRTLVFAEKTKADIIANARKEAEN
                     IIHEAELKATRAIQEAKQYLAVLEQQYIQLKEEKRQFLMQFRSELNTFKERIEKDPLL
                     AKEREPLLDADFKQIKEEISPTRKPTVEDNKN"
     gene            4375..5196
                     /locus_tag="PK015_04545"
     CDS             4375..5196
                     /locus_tag="PK015_04545"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOL33769.1"
                     /translation="MNLDYQFTASWLKEQLPLIPSVAMILGTGLNAIAEQSEIRAQIP
                     YAEIPGFVSTTAPEHKGNLILGELKGIPMIFLQGRFHYYEGYPMPMVVFPTRVLAALG
                     IRTLIVTNASGSLRENLSPASLVLITDHINFMGTNPLIGPNDDALGERFPSMNNPYDQ
                     QYLEQCEKIAQHKGIPIHKGVYIAVSGPSLETKAECAAFASWGADLVGMSTVPEVIVA
                     RHSQMRVLGFSIVTNYSNLFHNQAHSQEEIRHNAEKASANLLLIIAEFVQNIHFS"
     gene            5273..5674
                     /locus_tag="PK015_04550"
     CDS             5273..5674
                     /locus_tag="PK015_04550"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOL33770.1"
                     /translation="MAAKSMSPEKLKKFEELIQQELKESIAYIEEVSKGQSLGAKESS
                     GDLSSYAFHQADQGSDTHLMEQSVMLMEQEREKVRLLKEALQKIYDGTYGICEMCGKN
                     ISESRLEIIPYAHYCVNCMEKMEEKKRKQRK"
     gene            5678..6247
                     /gene="lspA"
                     /locus_tag="PK015_04555"
     CDS             5678..6247
                     /gene="lspA"
                     /locus_tag="PK015_04555"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOL33771.1"
                     /translation="MAKVKTYPAYLVMSLVIILDQVTKAVVRNKLPLYSWINVGEKWF
                     GDTFQIFHLQNSGAAFSLSLPNPLWNKGFFIAVSVIAIIFIIWLLYQSTNRIQVIAFG
                     LVLGGAIGNNLIDRPIFGAVTDFISVDIPNIIKGMDRFPVFNVADSAIFIGMCLLIYD
                     LIFISGKKAERKEAEPLLETNDKILDKEL"
     gene            6247..7686
                     /locus_tag="PK015_04560"
     CDS             6247..7686
                     /locus_tag="PK015_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33772.1"
                     /translation="MKKILLCALVLTTAFGLFAQVYTCHDIQYTTSASGDSPYVGRTV
                     TVRGIVVVELFYTGTNTSNIGFFISDPEGGPWSGLLIYTNQFHPQRGDEVEVTGTITE
                     YYNFTEMSPTTGYRVISQGNPIPPPTLINTGDLSSSTTAEQWEGVFVKVENVTIVSLP
                     TSYNEFYVNDGTGQCQIDDQCFPRTGFSWPQMTVGERWARIQGVVDYSFSNYAINPRD
                     MNDIVKEDNVSNAMIKVQTTTADINTETDIPILTSRIKPEWFVSSYTTNIKIDASKVK
                     FISVKYGTGTMTAQEPTVELSEDGSLIHISYQSMEPITSTADEDVLFKLCLKPVDYGE
                     SVIDLVSFFYDNTQIQALTDGKILVPIRKSIAWLNIKNPSSNKNIFNPELNEKLTIEY
                     GCKSSSSGINVKAIVRIYDAQGRLVATPINKNISSPLGIESFQWDGRDYMMRRVPIGL
                     YYCHLEIIERSTGHKEETVQPIVVKSIMK"
     gene            7705..8547
                     /locus_tag="PK015_04565"
     CDS             7705..8547
                     /locus_tag="PK015_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33773.1"
                     /translation="MKKILILAFILMQTALLLAVFDDYIPSARARALGGAYTSVADDV
                     NSLFFNPAGLINVNYEAQAGFSRLYNQKFSEYKTVAAGVKLPKKLGTVAIGARLFDVN
                     FEDENLMSEQIWSAGHSFNLQSDVHSQISVGYALNYYRLDFSEEEADDAFGLDLGATA
                     ILHQRTKLGFSVSNINQAVMGDTNQNELPSRLALGLSYIPYNQVITTIEVKKDFAKET
                     EFMGGVEVRLLEPLAIRFGVHSNPATWNAGVGFDLKGLNLDLSYSTHAVLGGTLYGNL
                     GYKF"
     gene            8560..8829
                     /locus_tag="PK015_04570"
     CDS             8560..8829
                     /locus_tag="PK015_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33774.1"
                     /translation="MAKFNFKKIFPLILPLFIEVSRYLIPNIIAPVKTKSNDTISTLE
                     NLLVRMERKVQENRLQVYKLRKLIIVWSTINSALLVAILVKLLFF"
     gene            complement(8924..9598)
                     /locus_tag="PK015_04575"
     CDS             complement(8924..9598)
                     /locus_tag="PK015_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOL33775.1"
                     /translation="MKIKYYGHSAVEITSNAGQKILIDPFLEGNPLSPVRADQVKADF
                     IILSHAHSDHLGDTIRIAKRNDAIVIAIAELAAYLAGKGLKTHSMQTGGSHIFPFGKV
                     KFTLALHGSNTPDGHYAGPAAGILLWVDDSCIYHCGDTGLFGDMKLIGEMNSVDYMLV
                     PIGDNFTMGPEDAIKAVEFVSPKIIIPIHYNTWPLIQVDAEAFAEKVKALGKECILLK
                     PGEEIP"
     gene            9738..9815
                     /locus_tag="PK015_04580"
     tRNA            9738..9815
                     /locus_tag="PK015_04580"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9773..9775,aa:Pro,seq:ggg)
     gene            complement(10204..11538)
                     /locus_tag="PK015_04585"
     CDS             complement(10204..11538)
                     /locus_tag="PK015_04585"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HOL33776.1"
                     /translation="MKSFKLFIVIILLVACAGPIFAQSLTIHISPHRYLSKDKATIVN
                     LDYQVPYNNLKFVKHKNGYYAELDINIEVLKNDSLIFQKNIRDNIGISNQFDAKSDKA
                     YLNRVSFSLDPGSYIFRVNALDINSKKSSTYSFPIETLSPETILSDIELCSVVRPDSS
                     AFLEKFHRGKTLYQSQPSLIFEKRDNPYVYLSFEVYTNESMRQDVGILILTVQKDSIL
                     IVDDMIDFIAHSDNERITLTIPLTDIEAGKYNGNITLQIGELSEERNFEFFVTEPKIQ
                     YYYVFVNPDDDYKLLRYFMGESAPGNWKTYSYDAKKQYVNRCWVKLANANQVSPESFI
                     QTIRERVDYANKHFSHFSDGWTTDIGRIYIRHGEPDDVEKGTTLDDTRFVRKDFQIWK
                     YRSRFNAVYLFLDIQMNGNYKLIYVNGDERESSNPNYMYYLGDDFDTSLLRN"
     gene            complement(11525..12640)
                     /locus_tag="PK015_04590"
     CDS             complement(11525..12640)
                     /locus_tag="PK015_04590"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HOL33777.1"
                     /translation="MKKAAKLALLFILISSSLLAQEVIYEHYADGVDFWVLIPYNSLV
                     FKHNTNSAVYNLSIEIKNKKTKDKFNYEETISVPRREWLQDTAIPIHFQAELGPGKYE
                     TLLRFKNLNLGNRTDLKRTFILDNKYTSIGQPFIFVFKEGIQFQPSDLTRLPSEVDSC
                     ILQQKFSVLPDSIQVFSSLHSQIFDYPRGKYQCDLTLWVNQDNMDFLKIVFFEDNVFY
                     EMEPFYYSPWFYFNAIYSYEDQIQQIRYIANQNEWNKIRKASPEEYPEIIEQFWQNHD
                     PSPGTTRNEFRESFYQRVRIADEKFTVHKKLKGWKSDRGRIYIKYGEPDEVYSEVHPL
                     DLKPYIVWVYYKPNRVFVFMDRGGYGQYELRNKDEEF"
     gene            complement(12624..16061)
                     /locus_tag="PK015_04595"
     CDS             complement(12624..16061)
                     /locus_tag="PK015_04595"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOL33778.1"
                     /translation="MTFVHLHNHTQYSLLDGACRVDRMVKLAKEYGMPAVAITDHGNL
                     YGAIDFYLTAKNEGIKPIIGMEAYIINGELDDEHSKYDPRYHLILIAKDYTGYKNLIK
                     LSSISFIDGFYYKPRISKSILKQYNEGLICLSGCIKGEIASLIAQHKMAEARETIEWY
                     QSVFGDRYFIEIQNHGLDEEKSVMPALISLAKEMHQPMVLTNDCHYLHKEDYEAHDIL
                     LCIQTGKSLNDPNRMRYETNQLYFKTPEEMKSIFPEEEEAYTNTLKIADMVDLELRYD
                     KFMLPKIDTPSEYKDMGEYLRALCEEGLKLKYPEPNKEILDRMNFELDVIHRMGFDGY
                     FLVVKDLIDNARKQGVPVGPGRGSGAGSIVAYLLNITQIDPIKYGLLFERFLNPDRVS
                     MPDIDIDFCAQGRSKVIDYLIQKYGRNSVTQIVTFSTLQAKSVFKDVARVLMLPASEA
                     NNITKTIPFGKSLEEAYQDSPDFRHLIDNNEIYQSIYKHSLVLEGLIRQTGIHPAGVV
                     IAPGDLKEYVPLTRSTQKGAENNILVQYEGKWLGELKFLKMDVLGLKTLTLIQKTIDM
                     VKESQNIDIDIDNLPLTDKKVYQLLSRGDTDGIFQFESEGMRKYLMDLKPNMFEDLIA
                     MVALYRPGPMKFIDTYIARKHKREKVTYDHPIMEATLKETYGVTVYQEQVMRISMEMA
                     NFTRGEADSLRKAMSKKSLDLLMQFQEKFIQGAISNGVPENVAAKIWNDWLDFAQYAF
                     NKSHATCYALVAYRTAWLKAHYPVEFMAALLSLEDDPVKIPVKIEVCKSMGIKILPPN
                     INKSEREFSVHGKEVLFGLKAIKNLGEAAINAIVEERQKNGPFTNIYNFCSRLDSTAV
                     NKTVLESLIASGAMDELEGTRAQKWAVIEEALSYNTGEQRDKRVGQTSLFDIISEDDE
                     SEDYPPLPEVEKWSYSYQLEKEKEVLGFYLGGHPLYEYRHLIQYLSNANSKSTRELES
                     KNLRLVGIITSINRKRDNKGNLYAFVEFEDLSGPFEVALFNKDYETFYPLLVTGKIYF
                     ISGTKSGYTGNDESLLRISPQKIVPLEDWNNILKGELRLNLPLGKLSTRFLEEFAKLV
                     NNNEGNFALKTIIYTDDGDNYKLEAAKHIFPSNALLELLVNNRLDFNLRLFEDEKSG"
     gene            16250..16765
                     /locus_tag="PK015_04600"
     CDS             16250..16765
                     /locus_tag="PK015_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33779.1"
                     /translation="MKRLYLLMLLVLLAIGLNAQRGLFGIAYDAPLNTADSLLCYQGF
                     LASEIEGNLITYRSDYNNFVDAIILIVDPIKEIVVGWFVSYNKDNTKEQDEYVLEQLE
                     KIHGKAVYLDQEREKICWIFDEHRVAIYGYAPSGNLCIYYYDFSYPELFQLPPTVEQK
                     IKNNPPDISQP"
     gene            16865..17926
                     /locus_tag="PK015_04605"
     CDS             16865..17926
                     /locus_tag="PK015_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOL33780.1"
                     /translation="MKTGGCRYGTHRVLEPKGVLPQPARVLDNDMSEIWDNELLINVT
                     RLNIDSASFHQIKKKLEEQGHTDLEKAFAEHTIDLVNRTGKHMNEDTGSGGMLIGTVA
                     QIGDKFEMKEEIKVGDKIATLVSLSLTPLKIDKVKRVLLDKDQIEVEGQAILFSSGVY
                     AKLPDDMDETLALAVFDVAGAPAQVERLVKPGDTVVILGANGKSGILCNAVARERAGI
                     TGKVIGVVRNPNYIPTCKETGCNEVIIANATDALTIQKEVSRLTNGKMADVVINVVNI
                     EDTELPSIMACKDRGIVYFFSMATSFTKAALGAEGIGADVDMMIGNGYAHHHSEIALD
                     LLRRNPVLMRIFKERYGEN"
     gene            17940..19187
                     /gene="ablA"
                     /locus_tag="PK015_04610"
     CDS             17940..19187
                     /gene="ablA"
                     /locus_tag="PK015_04610"
                     /EC_number="5.4.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050066 - lysine 2,3-aminomutase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046440 - L-lysine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HOL33781.1"
                     /translation="MTNTINIRSIATQDEWNDWHWQIRNRITDFETLRKYIELQPEEE
                     AVTQSKTFSFRMAITPYYLSLINPNNPLDPIRLQCIPRIQESYIAPTDLRDPLEEDAD
                     APVPGMTHRYPDRVLLLLTDQCGMYCRHCTRRRKAGETDAPMPKENVEKAIQYITEHK
                     EVRDVILSGGDPLTLSDERLDSVLDRLAKIDHIDIVRIGTRLPVVLPQRITDSLISIF
                     KKYKFLWLNTHFNHPHELTNESSAALAKLAEAGIVLGNQSVLLKGINDNVDVMKALVH
                     GLVKNRVRPYYIYQCDLSEGISHFRTPISKGIEIMESLRGHTSGLCVPTYVVDVPGGG
                     GKIPVMPNYVISQMPGRVILRNYEGMIISYTEPDFVVEDDSKYQSLCAPEYQSTEGVM
                     ALLRGKKVFIGPSDTARMKRRKS"
     gene            19190..20230
                     /locus_tag="PK015_04615"
     CDS             19190..20230
                     /locus_tag="PK015_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33782.1"
                     /translation="MAKFAGSSHFNWLEEESVKAITIVGICKNAGKTTLLNHLISFKK
                     KGTWGVFSTGIDGEETDFLFRIPKPPVILDKDFIFCCDTSTLDEFGCQIVVLSKLPFS
                     VDRPLWLAKTLIPLQTEITGPSTVKEQIQTLKLIQNYGAEKVLIDGSIDRKSIAQSEY
                     IDAVIMVIGANFGTLDEITDEVNRLKILNSIPQCTMVTPNSPEYKFLLDSEEICLYEE
                     RNWKPSGLVSLIGNIKPLRQMISKSTEAIYIPGAITDMVYQDIKNILSQSGAQLIVRH
                     PNCLKIRLKRLEFFYTQHHPTALIPFRIRNWALNSMGLGFSDIEAQEFRAYLRAQFPD
                     LNFVDVMELGYE"
     gene            20223..21137
                     /locus_tag="PK015_04620"
     CDS             20223..21137
                     /locus_tag="PK015_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOL33783.1"
                     /translation="MSDRDKITGKTVNLGLFCNVGLAIIKSIVGIIGHSQALLADGIN
                     STSDVVYYLAVKFFMRQANKPADSEHPYGHRQLESISAIVVGAFILTTGIAIFWQSLN
                     KIFDLVTGVEPFQSASTFVVLIAFLTLMLKIYLYFYTKKNFTLTKNPTLLALAKDHLN
                     DIMASGAVIVGVIMAQFGILWMDPAAGAIVAIFIVRTGISIIFQSASELMDAVPDKEF
                     GNIVRELTLSVEGVRDIEDLGVHRFGPSYTIEMIIGVDGNITVDEGNKISHKVEERLM
                     NHFHDSLRRVTIHFHPASSNKGPFFKAS"
     gene            21279..22472
                     /locus_tag="PK015_04625"
     CDS             21279..22472
                     /locus_tag="PK015_04625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOL33784.1"
                     /translation="MNKSREEQPNRKKKLLLITDLYPHRYNPVCGVFVKEHANELSKY
                     YQVKVIATSFPYPLKTEHKQEKDIEITHIFFPSIKSIFVTSFITYPLFALPKVKNIIK
                     SWNPDIIQVHDYHHIPELFWLKTWLDHTNFHIYLYLHNIRSHPDRLKGNRLIPFYRKT
                     LSKAFRKWDHIFTVNPRLKDWILPYMPENKITVTGNGISPTPPIQPEELLFIRNLCIP
                     DAYKIISVGNLLEAKGFGYLILAVYKLLQKGLNIQLVIVGEGKDHQRLTELIQNLNLS
                     DRVLLTGAIENSILRNIYPLFDLFVLASYSETFGIVFLEAMYASLPVIGIKGEGIYGL
                     FEEDKEALYAEPRNSEDLANKIEWLITHPEKAAAIAKAGQQKVREQFMLSDLIKKVRE
                     VYEQS"
     gene            22480..22764
                     /locus_tag="PK015_04630"
     CDS             22480..22764
                     /locus_tag="PK015_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33785.1"
                     /translation="MIINSFPPSGGSGVQRPLKFLKYSCHTGWEVHAIVPKKPTNTII
                     DYSLLAEIPAEAHIHKVRGLGIKIPEYAKITVLALKKLLPKILLKDSFGV"
BASE COUNT         7362 a         4039 c         4085 g         7292 t
ORIGIN      
        1 aaaggaaccg caaaactctt atattttaaa taaaccttca cctacacctt aggcacataa
       61 ataaaacata acttagatag gagagtcaaa atggctctcc tttctttttc cttctatatt
      121 tgtttttata ataccaaagc aaggtaaaga taagcatatt actaaataaa atccaaatca
      181 atgagtttga ttgtacacca ctcttggaaa ttcaacttaa tattgatgct atcgaaaaaa
      241 gctatagacc aaatatactt tgaactatca ggtgaagtga tgggataaaa tacatcctca
      301 ttttatggat aatgttataa agaataaatc tttaaaatta tttaataaat actctatctt
      361 tatttcgccc tttaatgtag aaactatctc aaacttatct ctaactttcg tcccattcgg
      421 acataagctg gacatgagtt ggacataagc tggacataag ctacccatct ataggcagag
      481 aaattttacg ggatataggc ttgaattgat tattttaatt attcttctgt caatattctt
      541 atctaccctc aacttccaat aggaaacaag gtctatttat gaagattata agctattcta
      601 taatagcata tagattttgc tttttatata taaacttccc tgaggaaaag attgacaaaa
      661 gaaagccacc aataaagaag gaagtttaat aaactaaaac ttttaggagg atatatgtcc
      721 tatcgtattc tcgctattaa tcctggttct acttccacta aaattgccgt atatgaagat
      781 gaccaaccca ttttagaaaa aaccttgcgt catagtccag aagaacttgc tccttttccg
      841 gacattattg accagtatga attccgcaaa aaaatcgttt tagaagcact ggcagaagga
      901 aatattgcac ctgaaactct tagtgctgtc gtcggaagag gaggtttaat aaaacctgtt
      961 cctggaggca cttttaagat taatgaagca atgaaacgag atcttcgcaa tcctaattta
     1021 tggggtagag ttcatgcatc caatcttggt gcttttattg ctgattccat tgctaaagaa
     1081 tataatattc ctgggtttat tgtagatccc gtagtagtag atgaatttga agatattgct
     1141 cgcatctcag gaattccaga gataaaaaga aactcccttt ttcacgctct taatatcaga
     1201 tatatctcca gacttttagc cagagaactc aatcaaaccc ttgaggaatc aaactttata
     1261 ggagtgcata tgggaggtgg tatatctgtc gccgctatta aaaatggaag agtggtagat
     1321 gttaataatg cactgctcgg tgatggacct ttttctcctc aaagagctgg tactctacct
     1381 attggtgatt tggtagaact tgctttcagc ggaaaatatt ctcactctga actggtaaaa
     1441 tatctatcta aaaatgccgg atggatggca tatctcggaa cagataatgg aattgaagtt
     1501 tataatcgta ttcaaaatgg tgatgcttat gcaaaattga ttaccgatgc tatgtgctat
     1561 aaagtggcta aagaaatagg tggatgtgct gctgttttag agggtaaggt tgatgctatt
     1621 ttctttagcg gtggtctctc ttttaatgaa tatatcgttg aacaaattaa atccagaatc
     1681 caattcctgg ctcctatcta tttatatccg ggagaaaaag aaatggaagc tctcgctcaa
     1741 ggtggattaa gagtgctcag aggtcttgaa gaagctcaag aatattcctg aacttaataa
     1801 taattattca ctcagctctt attttccctg atggtaaaat gtgttaatac catcttttct
     1861 gattatctaa ttatcaagat tattgattgc atcaagaaat ggaaattgag tcttatttcc
     1921 tgattttttt aaagtgcagt ttcttataat caatactata atttaatgaa taacaagaga
     1981 taaaggaatg tggataagtt gtggataaat tgtgtagaaa aatatgacta tgccttaact
     2041 tcctgtaaaa caatatgtta taagaaatat ataatccaca ctttttgtgg ataactccat
     2101 aattattagc ataataaggt agttagaaag tgaatacaga accctctttt ttaagcaaaa
     2161 accggattaa agaacttgca agattaaaac aaaaagacaa ccgattgcaa gaaaaactgg
     2221 ttgtagtgga tggatttcgc acacttgagc agataaagtt ttatggtatc aaccctctgg
     2281 agcaatattt acttgaggga atgcaaccaa taccaatttg ggataatatt ccagtttatt
     2341 acataagcga atgggatatg cataaaattt gtgacagtga acatccttct ggttttgcag
     2401 cacttttccc tattcctcag gcacagaaag tcaactttaa ttgtgctttt tacttagata
     2461 gaatcggtga cccaggcaac ttgggaagta ttttccgcat tgcagctgca ttttcaattg
     2521 atacactttt actatctcct gattgcgtag aagtttcatc acctaaagtc attcgttcct
     2581 cttctggtag tgtttataaa gttcctttcc gacaagccaa tatttcagaa ttaaaagcac
     2641 ttaagggaag aattattctt gctgatgccc ataaaggaca agctttaacc aaatttaact
     2701 tccagccaga ggaaaaatta attattgtac tcggttcaga agctcatgga atatcttcag
     2761 aggtttcttc actaaccaat gaagccttac atatagaaat gagctccgcc atagaatcct
     2821 taaatgtttc aatttctgcc gctataatag catatcatct tttcatccag agaaacacta
     2881 ataatttgac aaaataaagg tctattccat aaaggataaa taaggcttta atctctaagg
     2941 agctatagtg aatataatta ataataataa gactggttgg atagaagtta tctgtggaag
     3001 tatgtttagt ggtaagacag aagaactcat tcgtcgtatt cgtcgtgctg aatatgcaaa
     3061 acaaaaagtt atggtcttta aagcttctat tgataatcgt tatgatgcaa ctgacatcgt
     3121 gtcccattct caaatgcgtg ccccttctct tcctattgtg aagccagaag aaatctatca
     3181 atatctaacc gacgatataa caatagtagc tatagatgag gctcaattct ttgatgagac
     3241 cattgtagat gtatgtaatg atttagcaga taaaggttat cgtgtgatag ttgcaggatt
     3301 agatcaggat tatcgcggaa aacctttcaa tagtatgcca cagctgatgg caattgccga
     3361 atatgtgaca aaagttctgg ctatctgtgt caaatgtgga aatccagcta atcggactca
     3421 acgcacagtt caccaagatg accagattct ggttggaagc agtgaagctt atgaaccccg
     3481 ctgtcgtaac tgtcataagg tcttagattg atgatgggaa gaactatttt aggtgacatc
     3541 gcaggattta tagccagttt gctggatatt tatatcctga ttatccttat ccgagccttg
     3601 atgagctggt ttatgcttta tcatcagagc aaattttatt attttcttgt tcgtatcacg
     3661 gaacctgttt tagctcctat aagacgaata ctacccagaa tgagagttga cttctcaccc
     3721 gtgatagcta taattcttat tcaaataatt atcagtatct taaatcttat aaaatgagtt
     3781 tttttaagga ggtatgatga ccttgtttcc aactgatata cggcatcagg aattttccac
     3841 cactatgttt ggttatagca aaaaggaagt gaaagaattt ctggaacaac ttgctaatga
     3901 actggaagat tatcaaaggc gacaggaaaa agaactgcaa cgccgggaac aaatgcaagt
     3961 ggaagttcaa caggaagcta ttcaggcaag tagtgcagtg gaagaattga aaagaaggga
     4021 agaattaatc agtcgcacac tggtctttgc tgaaaaaaca aaggcagata tcattgctaa
     4081 tgcaagaaaa gaagcagaaa atattattca tgaagctgaa cttaaagcta cccgtgctat
     4141 acaggaagcg aaacaatatc tggctgtgct ggaacaacaa tatattcagt taaaagagga
     4201 aaaacgacag ttcttaatgc aatttagaag tgaactaaat acattcaagg aacgcattga
     4261 aaaagaccct ctcctggcaa aggagcgaga acctcttctg gatgcagatt ttaaacagat
     4321 taaagaggag attagtccaa cccgtaaacc cactgtggag gataataaaa actaatgaat
     4381 ctggattatc agtttacagc aagttggttg aaagaacaac taccactaat cccttctgtg
     4441 gctatgattc tgggaacagg attaaatgct attgcagaac aaagtgaaat cagagctcaa
     4501 atcccctatg ctgaaatccc cggttttgtt tctactactg caccagaaca taaaggcaat
     4561 cttattctgg gagaactgaa aggtattccg atgatatttt tacaaggacg ctttcattat
     4621 tatgaaggtt atccgatgcc aatggtggtc ttccccactc gcgttttagc tgctctggga
     4681 ataagaactc taattgtaac caatgcttca ggaagtttac gcgaaaatct ctctcccgct
     4741 tcactggtac tcattactga ccatatcaac tttatgggaa ctaatccttt aattggaccc
     4801 aatgatgatg cacttggtga aagattcccc agtatgaata atccttatga tcagcaatac
     4861 cttgagcaat gtgaaaagat agcccagcat aaaggtattc ccattcataa aggtgtctat
     4921 attgcagtaa gcggtcccag tcttgagact aaagcagaat gtgctgcttt tgccagttgg
     4981 ggtgctgact tagtaggtat gtccacagtt ccagaagtta ttgtggctcg tcattctcaa
     5041 atgcgagtac ttggtttttc tattgtaact aattatagta atctattcca caatcaggct
     5101 catagtcagg aagaaattcg tcataatgca gaaaaggctt ctgctaattt actgctcata
     5161 atcgctgaat ttgtccaaaa tatacatttt tcataagtat attttcattg acataactta
     5221 gtatatagga ttgaaggaac tttaaatatt ttatttagca caggagtgaa agatggctgc
     5281 aaaatcaatg tctccagaga agttgaagaa atttgaggaa ctcattcagc aagaattaaa
     5341 agaaagtatt gcttatattg aagaggttag taaaggtcag agtttaggcg ctaaagaaag
     5401 cagtggtgat ctttcttcct atgcctttca tcaggctgac cagggttcag atacacattt
     5461 aatggaacaa tctgtaatgc ttatggaaca agaacgagaa aaagttcgtt tactgaaaga
     5521 agctctacag aaaatctatg atggaactta tggcatctgt gaaatgtgcg ggaaaaatat
     5581 tagcgaatcc agattagaaa tcattcctta tgctcattat tgcgttaact gtatggaaaa
     5641 gatggaagaa aagaagcgta aacaaagaaa atagctaatg gctaaagtta agacttatcc
     5701 ggcttatctt gttatgtctc tggtcattat tctggaccag gtaactaaag cggtggtccg
     5761 aaataagcta ccgctttata gctggataaa tgtcggtgaa aaatggttcg gagatacctt
     5821 ccagattttt cacttgcaaa atagtggagc agcttttagt ctatccttgc ccaatccttt
     5881 atggaataaa ggttttttca tagctgtatc ggtgatagcc attatcttca ttatctggct
     5941 attatatcaa tccacgaaca gaattcaggt tatagcattt ggattggtgc ttggtggtgc
     6001 tataggtaat aatcttattg accgaccaat ttttggtgcg gtaactgatt ttataagcgt
     6061 tgatattcct aacataatca aaggaatgga tagatttcct gtctttaatg tggcagacag
     6121 tgcaatcttt attggaatgt gtctattaat ttatgattta atctttatta gtggaaagaa
     6181 ggcagaaagg aaagaggctg aaccacttct tgagactaat gataaaatct tagataagga
     6241 gctataatga aaaaaatttt actttgtgct ctggtattaa ccacagcatt tggactcttt
     6301 gctcaggttt acacctgtca cgatattcaa tatactactt ctgcctctgg tgattctcct
     6361 tatgtaggta gaactgtcac agttagaggt attgttgtgg tagagctatt ctatactgga
     6421 acaaatacca gtaatattgg tttttttatc tctgatcctg aaggaggacc ctggagtggt
     6481 ttattgattt ataccaatca atttcatcct caacgaggag atgaagttga agttacaggt
     6541 actattactg aatattataa ttttactgag atgtcaccta ccacaggtta ccgggtaata
     6601 agtcagggaa atcccattcc tccaccaact ctaataaata ctggtgactt atcttccagc
     6661 actacagctg aacaatggga aggtgttttt gtaaaagtgg agaatgtgac cattgtttct
     6721 ctacctacaa gttataatga gttttatgtg aatgatggaa cgggacaatg ccagattgac
     6781 gatcaatgtt ttcccagaac tggattttcc tggccccaaa tgactgtggg agaacgctgg
     6841 gctcgtattc aaggagtggt agattattct tttagtaatt atgccatcaa tcctcgtgat
     6901 atgaatgata tagtgaaaga ggataatgta tcaaatgcta tgatcaaggt tcaaacaact
     6961 actgcagaca ttaatacaga aacagatatt cccatcttga cttcaagaat aaaaccagaa
     7021 tggttcgtta gttcctatac gacaaatatt aaaattgatg ccagtaaggt taagtttatc
     7081 agtgtaaaat atggaacagg aacgatgact gctcaagagc caaccgtaga attatcagaa
     7141 gatggctcct tgattcatat cagttatcag tcaatggaac ctattacttc tacagcagat
     7201 gaagatgtgt tatttaaatt atgtcttaaa ccagtagact atggtgaatc cgtcatagac
     7261 ctcgtttctt tcttctatga taatactcag attcaggctc taactgatgg aaaaattctt
     7321 gtgcctatcc gtaaaagtat agcctggcta aatattaaaa atccgagttc caataagaac
     7381 atcttcaatc cagagctaaa tgaaaaactt accatagaat acggttgcaa aagctcaagc
     7441 tctggcataa atgttaaagc aattgttcgg atttatgatg cacaaggacg tttagttgct
     7501 actccaatta ataaaaatat cagtagtcct ttaggaattg aaagcttcca gtgggatggt
     7561 cgtgattata tgatgagaag agttcctatt ggtctttatt attgtcattt agaaataatt
     7621 gaacgtagca caggacacaa agaggaaact gttcaaccta ttgtggttaa atctataatg
     7681 aaataagctc gaggagaaat gaaaatgaaa aaaatactaa ttcttgcttt tattctgatg
     7741 cagacagctt tgctgcttgc cgtatttgat gattatattc cttctgctcg ggctcgtgct
     7801 cttggtggag cttatactag tgtagcagat gatgttaatt ctctattttt caatccagca
     7861 ggtttgatta atgttaatta tgaagctcaa gcaggattca gtcgtctcta caatcaaaaa
     7921 ttttcagagt ataaaacagt agctgcaggt gttaagcttc ccaagaaact gggaaccgta
     7981 gctattggag caagactgtt tgatgtcaat tttgaagatg agaacttgat gagcgaacaa
     8041 atctggtctg cgggtcattc tttcaatctt caaagtgatg ttcattcaca gataagtgtg
     8101 ggttatgctc ttaattatta tcgtctggat ttttccgaag aagaagctga tgatgctttt
     8161 ggtttagacc tgggagctac ggctatactt catcaaagaa ctaaacttgg ttttagtgtg
     8221 agcaatatca atcaagcagt tatgggtgat actaatcaaa atgaattacc gagtcgttta
     8281 gctcttggac tttcttatat cccatataat caggtgatta ccacaattga ggtaaaaaag
     8341 gattttgcca aagagaccga atttatggga ggagtggaag tccgcctgct ggaaccttta
     8401 gcaattaggt ttggagtgca ttccaatcca gctacctgga atgcaggtgt gggatttgat
     8461 cttaagggac ttaacttaga cctatcatat tctactcatg ctgttctggg tggaacactt
     8521 tatggtaact taggatataa attttaagga gtaagctcta tggctaaatt taattttaaa
     8581 aagatattcc cgctcattct acctttattt atagaagtga gtcgttattt aataccaaat
     8641 ataattgcac ccgtaaagac taagagcaat gacacaattt caacactgga aaatctatta
     8701 gttcgtatgg aaagaaaagt gcaagaaaat cgtctgcagg tttataaact aagaaaacta
     8761 attattgtgt ggtctactat aaattccgct cttttagttg caatattggt gaagcttcta
     8821 ttcttttaat taaaatacta cccccttctt ttcttattta gatgaaagaa aaagaagggg
     8881 gtattttttt ctttttaata gttttctaaa tctctttttt tgatcagggt atctcttctc
     8941 ctggtttaag tagaatacat tcttttccca gggcttttac tttttcggca aaggcttcag
     9001 catcaacctg aataagaggc caggtattgt aatgtattgg aattataatc ttaggtgata
     9061 caaattctac tgccttgata gcatcttcag gacccatagt aaagttatct cctattggaa
     9121 ccagcatata atctacagaa ttcatttccc caatcaattt catatcacca aaaagaccgg
     9181 tatctccaca atgatagata caggaatcat ctacccataa taaaatgcct gcagctggtc
     9241 ctgcataatg tccatcagga gtgttacttc catgcaaagc aagggtgaac tttaccttac
     9301 caaacgggaa aatatgactt ccgccagttt gcatactatg tgtttttaat cccttacccg
     9361 ctaaatatgc tgctaattct gctattgcta taactatcgc atcatttctt ttggcaatcc
     9421 tgatagtatc acctaaatgg tcactatgag catgagaaag aattataaaa tctgctttaa
     9481 cctggtcagc tcttaccggt gaaagtggat tcccctctaa aaaggggtca ataaggattt
     9541 tttgccctgc attactggta atttcaacag ctgaatgacc gtaatattta atcttcattt
     9601 ttcacctcct tactattata ttgaatttta tagtaaaaaa gtcaaggatt ttctaatctt
     9661 ttagaaaaga aactaaatta agttgcactt ttcccttgtc aaaaaacctt agttttagtt
     9721 ttgtgttttt tatggttcgg gatgtagcgc agtccggcta gcgtacttga atggggttca
     9781 agtggtcgca ggttcaaatc ctgtcatccc gaccattatt ttttgccata taatctctat
     9841 ataaaccatc aaaaagctat tattatctat atttgaaata gtatgtattt tctttcaaat
     9901 tgctattttc ttatttatct gccattcaat attttccaaa ctctatctta gctctttctt
     9961 ggctctctct tggctcttta attaaggaag acctaagaac aagcaaggaa taagaataga
    10021 atgatagtgt aatcataagt tatataaaga ataagtgata gtggtataaa gtgatttttc
    10081 tactcacttg gtgtattcta ttggtaataa tgagtattat gcttttatat aattatttta
    10141 ggaagacttt aataacttct atttctgaaa cctggagaga aaaactctta atttatcagg
    10201 atatcagttc ctaagtagag aggtatcaaa atcatcaccg agataataca tatagttagg
    10261 attagaactt tcccgttcat ctccattcac ataaattagt ttataattac cattcatctg
    10321 tatatctaag aaaagatata ccgcattgaa tctacttcta tatttccaga tttgaaaatc
    10381 tttacgcacg aaacgagtat catccagggt agtgcctttt tccacatcat ccggttcacc
    10441 gtgtctaata tatatcctac caatatctgt agtccatcca tcgctaaaat ggctgaaatg
    10501 tttattagcg taatccactc tttcacgaat agtctggata aaagattcag ggcttacctg
    10561 attcgcatta gccagtttaa cccagcaacg gttcacatac tgtttttttg catcatagga
    10621 ataagttttc cagtttccag gagcactttc acccataaag tatctaagca gtttataatc
    10681 atcatcagga tttacgaaaa cataataata ttgaatttta ggttcagtaa caaagaattc
    10741 aaaatttcgt tcttcactaa gttcgccaat ctgtaaggta atgtttccgt tatattttcc
    10801 cgcttcaata tctgttagtg gtatagttaa agtgattctt tcgttatcgc tatgggcgat
    10861 aaaatctatc atatcatcaa cgattaagat actatccttt tgcacagtta gaattaaaat
    10921 tcccacatcc tgacgcatag attcattggt gtatacttca aaactgagat aaacataagg
    10981 attatccctc ttttcaaaaa taagagaggg ttgactctga taaagggtct ttcccctatg
    11041 aaatttttct aaaaaggcac tgctatcagg acgcaccaca ctgcacaatt ctatatcact
    11101 taaaatcgtc tcgggtgaga gagtttcaat aggaaaagaa taagtggaac tttttttaga
    11161 atttatatct aaggcattaa cccgaaatat ataggaacct ggatccagac taaaactaac
    11221 tcggttcaaa taagctttat ccgacttagc atcaaattga ttggaaatac ctatattatc
    11281 cctaatattt ttctgaaaga taagagaatc attcttcaat acctctatat taatatctaa
    11341 ctcagcataa tagccatttt tatgtttaac aaacttaagg ttattatagg gaacttgata
    11401 atccagatta acgatagtag ccttatcctt actcaaatac ctgtgaggac tgatatgaat
    11461 agtaagagac tgggcaaata tcggtcctgc gcatgcaact aacaagatta tcacgataaa
    11521 taatttaaaa ctcttcatct ttattcctta attcatattg gccataaccg cccctatcca
    11581 taaacacaaa tacccgattg ggtttgtagt atacccaaac gatatagggt ttaaggtcta
    11641 aggggtgaac ttcactataa acttcatctg gttcgccata cttaatatat attctaccac
    11701 gatcggactt ccaacccttc aattttttat gaacagtaaa tttttcatcg gcaattctaa
    11761 ctctttgata aaagctttca cggaactcat ttcttgttgt tcctggactg gggtcatgat
    11821 tttgccaaaa ttgctctata atttcagggt attcctctgg tgaagccttt ctgattttat
    11881 tccattcatt ttgatttgca atataccgaa tttgctggat ttgatcttca tagctataaa
    11941 tcgcattaaa atagaaccag ggactgtagt aaaaaggttc catttcataa aaaacattat
    12001 cctcaaagaa gacaatcttt aggaaatcca tattatcttg attaacccat aaagttaggt
    12061 cacattgata cttgcctctc ggataatcaa aaatttgaga atgaagcgat gaaaatactt
    12121 gaatactatc aggtaaaaca ctaaattttt gctgaaggat acagctatct acctcagatg
    12181 gtaatcgtgt taaatctgat ggctgaaact gaataccctc tttaaaaaca aatataaatg
    12241 gctgaccaat ggaagtatat ttattatcca ggataaaggt tcttttcaaa tcagttctat
    12301 tccccaaatt caagttctta aacctcaaca aagtttcata cttccccgga cctaactcag
    12361 cttgaaaatg tatgggaatg gcggtatctt gtaaccattc tcgtctggga acagaaatag
    12421 tctcttcata attaaattta tcttttgtct ttttattctt aatttcaatg cttaaattat
    12481 atactgcgga attggtgtta tgcttaaaaa ccagagaatt ataaggaata agcacccaaa
    12541 aatctacccc atcagcataa tgctcatata taacttcctg agctaataaa gagctgctaa
    12601 ttaaaataaa taaaagtgcg agcttagccg cttttttcat cttcaaatag cctcagattg
    12661 aagtctaatc tattatttac cagtaattcc agcaaagcat tagagggaaa aatgtgtttt
    12721 gctgcttcca gtttataatt atccccatca tcggtataga taatagtttt aagtgcgaaa
    12781 ttaccttcgt tattatttac caatttagca aattcttcca gaaaacgcgt agataatttt
    12841 cccagaggta aattcaacct aagttcaccc ttcaaaatgt tattccaatc ttccagagga
    12901 acaatttttt gtggacttat ccgtaagaga ctctcatcat tgcctgtata acctgattta
    12961 gttcctgaaa tgaaatatat tttgcctgta accagcagag gataaaaagt ttcataatct
    13021 ttattgaaga gagccacttc aaagggtcca gataaatctt caaattcaac aaaagcatag
    13081 agattaccct tattatccct tttcctgttt atagaagtaa ttatccccac caaccgaaga
    13141 ttcttagatt ccagttctct ggttgattta gaattggcat tagataaata ctgaataagg
    13201 tgacgatact cataaagcgg atgaccacct aagtaaaatc cgaggacttc cttttctttt
    13261 tccaattgat aggaatagct ccatttttct acctcaggta atggtggata atcttctgat
    13321 tcatcatcct cactaattat atcaaagagt gaggtttgac ctactcgttt atctctttgt
    13381 tcacccgtat tataagatag agcttcttct ataactgccc atttctgagc acgtgttcct
    13441 tccagttcat ccattgcacc actggctatt aagctttcca gtaccgtctt atttactgct
    13501 gtgctatcca aacgggaaca gaaattatag atattggtaa aaggaccatt tttctgccgt
    13561 tcttctacga ttgcatttat agcagcttca cccagatttt tgatagcctt caatccgaac
    13621 aatacttcct ttccatgaac actgaattcc ctttcacttt tatttatgtt tggaggtagt
    13681 atcttaatac ccatagactt gcatacttct atctttactg gaatcttgac tgggtcatcc
    13741 tctaaagaaa gtaatgctgc cataaattca actggataat gcgctttcag ccatgcagtt
    13801 ctataagcga ccaaggcata acaagtggca tgacttttat taaaagcata ttgagcaaaa
    13861 tctaaccaat cattccagat tttagccgct acattttcag gaacgccatt actaattgca
    13921 ccctgaataa atttttcttg aaattgcatc aagaggtcaa gactcttttt gctcattgct
    13981 tttctaagag aatctgcttc acccctggtg aaatttgcca tctccataga aatgcgcata
    14041 acctgttctt gataaaccgt aactccatag gtctctttta aagttgcttc cattatagga
    14101 tggtcgtaag taaccttctc tcttttatgt ttacgagcaa tgtaagtatc aataaatttc
    14161 attggaccag gacgataaag agccaccata gcaatcaaat cttcaaacat attaggtttt
    14221 aaatccatca gatacttacg cataccttcc gattcaaact gaaaaattcc atcggtatct
    14281 cctctactta aaagctgata gactttttta tccgtgagag gtagattatc aatatcaata
    14341 tctatattct gtgattcttt taccatatca atggttttct gaattaaagt gagagtcttt
    14401 aaaccaagaa catccatctt caagaatttc agttcaccca accactttcc ctcgtactgg
    14461 acgagaatgt tattctctgc acccttttgt gtactacgag ttagagggac atattctttt
    14521 aaatcaccag gagcaatgac tacacccgca ggatgaatgc cggtttgacg aataagtcct
    14581 tccagcacga gactgtgctt atagatgctt tgatatattt cattattatc aataagatgt
    14641 ctaaaatcag gagaatcctg ataggcttct tccagacttt ttccgaaagg aatagtctta
    14701 gtaatattat tagcttcact ggctggaagc atcagcactc tcgctacatc tttaaatacg
    14761 gacttggctt gcaaggtgct aaaagtgaca atctgagtga cactattccg tccatatttt
    14821 tggatgagat aatcaataac tttacttcgt ccttgcgcac aaaaatcaat atctatatca
    14881 ggcatactta cccggtcagg atttaagaac cgctcaaata ataagccata cttaataggg
    14941 tcaatttggg ttatattcag cagataagct acaatacttc cagctccaga gccacgtcca
    15001 ggacctactg gaactccttg cttacgagca ttatctatca aatctttaac cactaaaaaa
    15061 tagccatcaa agcccatccg atgaatcaca tccagttcaa aattcattct atccagaatt
    15121 tctttgttcg gttcaggata ctttagcttc aatccctctt cacaaagagc acgtagatat
    15181 tctcccatat ctttatattc ggaaggagtg tcaattttag gcagcataaa tttgtcatag
    15241 cgcagttcca aatcaaccat atccgcaatt ttcaaggtat tggtataagc ttcttcctcc
    15301 tccgggaaga tggatttcat ttcttcagga gtcttaaaat agagctgatt tgtctcatag
    15361 cgcatacgat tgggatcgtt taagctttta cctgtctgga tgcagagaag gatatcatgt
    15421 gcctcatagt cttctttatg caaatagtgg caatcattag ttaaaaccat aggttgatgc
    15481 atttcttttg ctaaagaaat cagagctggc ataacactct tttcctcatc cagaccatga
    15541 ttctgaattt ctatgaaata gcggtctcca aagacagatt gataccattc aattgtttca
    15601 cgagcctcag ccattttatg ctgtgctatt aagctggcaa tttctccttt gatacatcca
    15661 cttaaacaga taagcccctc attatattgc ttcaatatgg acttacttat acgaggtttg
    15721 tagtagaagc catctataaa agagatagaa gataatttaa taagattctt gtaacctgta
    15781 tagtccttgg caataaggat aagatgataa cgggggtcat atttactatg ttcatcatcc
    15841 aattcaccat ttataatata agcttccatc ccaataattg gctttatacc ttcatttttt
    15901 gccgttaagt aaaagtctat tgctccataa agattaccat ggtcagtgat agctacagct
    15961 ggcattccat attctttagc caatttaacc atacggtcta ctctacaagc tccatcaagt
    16021 agactatatt gagtgtgatt atgtaaatgt acgaaagtca taattatctt ttcctcttat
    16081 ggacaaatat taaaaagaag gctcgatttg tcaaagtata aattcaaact atcaatttag
    16141 ttctacttgc attattttgg attgataaaa gtaaaaacta actgttttat ggggttagat
    16201 agctgatttt aattgacgaa gaattgagat taaaatataa ggaaaaatta tgaaaagatt
    16261 atatttatta atgttgctcg tactgttagc tataggtcta aatgctcaaa gaggactttt
    16321 tggcattgct tatgatgccc ctcttaacac agcagattcg ctgttatgtt atcaaggttt
    16381 tttagcatct gaaattgaag gcaatctaat cacttaccgt tcagattata ataatttcgt
    16441 agatgcaatc attttaattg ttgatccgat aaaagaaatt gttgtaggat ggtttgtaag
    16501 ctataataag gataatacta aggaacagga tgaatatgtg ctggaacaac tggaaaagat
    16561 acatggaaaa gcggtatatt tagaccaaga gcgagaaaaa atttgctgga tttttgatga
    16621 acatcgtgtt gcaatctatg gatatgcacc ttctggaaac ctttgtatct attattacga
    16681 tttttcctat ccagaactat ttcaacttcc accaacggtt gagcaaaaaa taaagaataa
    16741 cccccctgat atttcccaac cctgatacca ttctttgaag ttataacctg ttgttctgct
    16801 tgacatttaa agataagcta aaaatcttga ataaagaaaa aaatcaataa taaaggagct
    16861 aattatgaaa acgggaggat gtcgttatgg aacacatcgg gttttggaac ctaaaggggt
    16921 gcttccacaa ccagccagag tgcttgataa cgatatgagt gaaatctggg ataatgagtt
    16981 gctcattaat gtaacgagat taaatattga ttctgcttca ttccaccaaa taaaaaagaa
    17041 attggaagaa cagggacata ctgacttgga gaaagctttt gcagaacaca caatagacct
    17101 ggtaaatcgc actggtaaac atatgaatga ggatactggt tcaggtggta tgcttattgg
    17161 aacagtagca caaataggtg ataaatttga gatgaaagaa gagataaaag taggagataa
    17221 gatagctact ctggtctctc tttccttaac tccattaaaa atagataaag taaaaagagt
    17281 tcttttagat aaagaccaga ttgaagttga aggacaggct atccttttca gcagtggtgt
    17341 ctatgctaaa ttacctgatg atatggatga aaccctggct cttgcagttt ttgatgttgc
    17401 aggagcacca gctcaagtag aacgtttggt taaacctggt gatacagtgg taatattagg
    17461 tgcaaatggg aaaagtggaa ttttatgtaa tgcggttgcc cgcgaaagag ctggaatcac
    17521 cggaaaagtt ataggagtgg ttcgtaatcc taattatata ccaacctgca aagaaacagg
    17581 ttgtaatgaa gtcattattg ctaatgcgac agatgcgcta actatccaaa aagaagtttc
    17641 tcgtctaact aacggcaaaa tggcagatgt ggttattaat gttgtaaata ttgaagatac
    17701 agaactgcct tcaataatgg catgtaaaga tcgtggcatt gtctatttct tttctatggc
    17761 tacttctttc accaaagcag ctcttggcgc tgaaggaatt ggcgctgatg tggatatgat
    17821 gataggaaat ggatatgctc atcatcattc agagattgct ctggatttac ttcgtaggaa
    17881 tcctgtttta atgcgtatct tcaaagaacg ctatggtgaa aattaaggag aaagtagaaa
    17941 tgactaatac aattaatatc cgttctatag caactcaaga tgaatggaat gattggcatt
    18001 ggcaaataag aaatcgcata accgattttg agaccttaag aaaatatata gaattgcaac
    18061 ctgaagaaga agccgttacc caatccaaaa ctttctcttt taggatggct ataactccct
    18121 attatttatc actaatcaat cctaataatc ctttggaccc aataagactg caatgtattc
    18181 ctcggataca ggagagttat atcgctccca ctgatttgag agacccttta gaagaagatg
    18241 cagatgctcc tgtgccaggt atgacacatc gctatccgga tagagtattg cttcttttga
    18301 ctgatcaatg cggaatgtat tgtcgtcatt gcactcgccg tcgtaaagct ggagagactg
    18361 atgctcctat gcccaaagaa aatgtggaaa aagccattca gtatattaca gaacacaaag
    18421 aagtgcgaga tgtaatcctt tctggaggcg accctctaac tctgagtgat gagcgtcttg
    18481 atagtgtttt agaccgctta gcaaagatag accatattga tatagttaga atcggaacaa
    18541 gactacctgt tgttttacct caacgaataa cggatagctt aatatccatt ttcaaaaaat
    18601 ataagttcct ctggttaaat actcatttca atcatcctca tgaattaacc aatgaatcca
    18661 gtgctgcctt agccaaactc gctgaagcag gaattgtatt gggaaaccag tctgttttac
    18721 tcaaaggaat taacgataat gtagatgtga tgaaagctct ggtgcatggt ttggtaaaga
    18781 acagagtgcg tccttattac atatatcaat gtgatttatc tgaaggcatc tcccatttcc
    18841 gcactcccat ctcaaagggt atagaaatta tggaatcatt acgtggtcat acttctggat
    18901 tatgtgttcc cacatatgta gttgatgttc ctggtggtgg tggaaagatt cccgttatgc
    18961 caaattatgt tatttctcaa atgcccggac gagttattct gcgtaattat gagggaatga
    19021 taatatccta tactgaacca gattttgtgg ttgaggatga tagcaaatat caatcacttt
    19081 gtgctccgga atatcaatcc actgaaggag taatggctct gttgagaggt aaaaaagtct
    19141 ttataggtcc ctcagataca gcacggatga aacgtcggaa atcttaatta tggctaaatt
    19201 tgctggttcc tctcatttta attggctgga agaggaaagt gttaaagcga ttactattgt
    19261 cggtatctgc aaaaatgcag ggaaaacaac cctcctcaat catttgattt cctttaaaaa
    19321 gaaaggaacc tggggagttt ttagtacggg aatagatggt gaagagactg atttcttgtt
    19381 tcgtattcct aaacctcctg taattcttga taaagacttc atattttgct gtgatacctc
    19441 aacattagat gaatttggtt gtcagatagt cgtgctgtca aaacttcctt tctcagtaga
    19501 ccgtcctttg tggctggcaa aaactcttat tcctttgcaa actgagataa cgggaccttc
    19561 tactgtcaaa gaacagattc aaactctaaa attaatccag aattatggtg cagaaaaagt
    19621 gctaatagat ggttctatag accgaaaatc catagctcaa tcagaatata ttgatgcagt
    19681 gattatggtc attggcgcca attttggcac tttagatgaa attacggatg aagttaatag
    19741 attaaaaatc ttaaattcta ttccacaatg tactatggta actcccaatt ctcctgaata
    19801 taagtttcta cttgattcag aggagatttg tctttatgaa gaaaggaact ggaaacccag
    19861 tggattagtc tctctcatag gtaatattaa accacttagg caaatgattt ccaagagcac
    19921 tgaagctatt tatattcctg gtgctattac tgatatggtc taccaggata taaaaaatat
    19981 attatctcag agcggagctc aattaattgt tcgtcatcct aattgcttga aaattcgttt
    20041 aaaaagactg gaattttttt atactcaaca tcatcctacg gcattgattc cttttcgcat
    20101 tagaaactgg gctcttaatt ctatgggatt aggattttct gatattgaag cacaagaatt
    20161 tagagcttac ttacgtgctc aatttcctga ccttaatttt gttgatgtga tggaattagg
    20221 atatgagtga cagagataag ataaccggaa aaaccgttaa tcttggtctg ttctgcaatg
    20281 taggattagc cattataaaa tctatagtgg gaattattgg tcattctcaa gcattattag
    20341 ccgatggtat caattccact tccgatgtgg tatattatct tgcagtaaag ttctttatgc
    20401 gacaggcaaa taaaccagct gactctgaac atccctatgg acatagacaa ttagaaagta
    20461 tttcagctat agtagttgga gcttttattc ttactacggg tatcgctatc ttctggcaat
    20521 ccctcaataa aatctttgat cttgtgactg gagtagaacc ctttcaatct gcttcaacct
    20581 ttgtggtatt aatcgctttc ttgactctga tgctgaagat ttatttatat ttttatacta
    20641 agaagaactt taccttaact aaaaatccta cccttcttgc cttagctaaa gaccatttaa
    20701 atgatattat ggcttctgga gcagtgatag ttggtgtaat aatggctcaa tttggaatct
    20761 tatggatgga ccctgcagct ggtgcaattg tggcaatatt tattgttcgt acaggaattt
    20821 ctatcatttt ccaatctgct tcagaactta tggatgcggt tccagacaaa gaatttggaa
    20881 atatagtgag agaattaacc ttgtctgtgg aaggagtacg agatattgaa gacttaggag
    20941 ttcatagatt tggtccctca tatactattg agatgattat cggagtagat ggaaatatca
    21001 ctgtggatga ggggaataaa atatcacata aggttgaaga aaggcttatg aatcatttcc
    21061 atgatagtct taggagggta actatacatt ttcatccagc ttcctcaaat aagggtccat
    21121 ttttcaaagc atcttgatac atatctttca caggaataat gaccaataat aaagatagta
    21181 tgatttaaat gataccacta catccgacat gtataacatt tatgggaaat aatcttattt
    21241 taactgtact cctttattta ttagggtata taaactaaat gaataagtcc agagaagagc
    21301 aacccaaccg taaaaaaaag ctattactaa taacagattt atatcctcat cgttataatc
    21361 ccgtatgtgg tgtttttgta aaagaacatg caaatgaatt atcaaaatat tatcaggtta
    21421 aagtaattgc aacatctttt ccttatcccc tgaaaactga gcacaaacaa gagaaagata
    21481 ttgagattac tcatattttc tttccttcaa taaaatcaat ttttgttact tcttttatca
    21541 catatcccct atttgctttg cctaaagtta agaacattat aaaatcttgg aatcctgata
    21601 taatacaggt tcacgattat catcatatcc cagagctttt ctggttaaaa acctggctgg
    21661 atcatactaa ctttcatatt tacttatatc ttcataatat tcgctctcat cctgatagat
    21721 taaaaggtaa ccgtctcatc cccttttatc ggaaaacttt atcaaaagcc tttcgtaaat
    21781 gggatcatat ctttactgtt aatcctcgct taaaagattg gattcttcct tatatgccag
    21841 agaataaaat cacagtaact ggaaatggaa tatctccaac tccacccatt caaccagaag
    21901 aacttttatt tatccgaaat ctctgtattc ctgatgctta taagattatt tctgtaggga
    21961 atcttttgga agctaaaggt tttggttatc ttattttagc agtctataaa cttctccaga
    22021 aaggtttaaa tatacagtta gttatagttg gagaaggtaa agaccatcaa agattaacag
    22081 aactaataca aaatttaaat ttatctgaca gagtattatt aactggtgct attgaaaata
    22141 gcattctgag aaatatatat ccactttttg atcttttcgt ccttgcaagc tatagtgaaa
    22201 cttttggtat tgtcttctta gaagcaatgt atgcaagctt acctgtaatc ggtatcaaag
    22261 gagaaggaat atatggacta tttgaagagg ataaagaggc tctatatgca gaacctcgta
    22321 acagtgaaga ccttgcaaat aaaatagaat ggctcataac tcatcctgaa aaagcagcag
    22381 ctattgctaa ggctggacaa caaaaagtaa gggaacaatt tatgttatca gatcttataa
    22441 aaaaagttag agaagtatat gaacaatcat aagttattaa tgattattaa tagttttccc
    22501 ccaagtggtg gcagtggtgt tcaaagaccc ctaaaatttc tgaaatatag ctgtcacacg
    22561 ggttgggaag tacatgcaat agttcctaaa aaaccgacta atactattat agactatagt
    22621 cttttagcgg aaataccagc agaagctcat atccacaaag ttagagggtt gggcatcaaa
    22681 atacctgaat atgctaaaat aacagtactt gctttgaaga aactactccc caaaatccta
    22741 ttgaaagaca gttttggcgt ctaataaaat tctttaac
//
LOCUS       DAORZF010000028        21216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_2117_length_21216_cov_379.427201, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000028 DAORZF010000000
VERSION     DAORZF010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 21216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21216
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2117_length_21216_cov_379.427201"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(369..2855)
                     /locus_tag="PK015_04635"
     CDS             complement(369..2855)
                     /locus_tag="PK015_04635"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOL33786.1"
                     /translation="MVWGFSSLLASYLPANFTNQGNPVLYKKVNKLAYSNLKKLDHFH
                     QQAYKKLLKEQDDILMSYLIAYESDAILQIANPEDVLSNYLQIRSYLNTYGTDLSPEF
                     FLSYVAHQTVADERITPYRAEFLRDGLREVLQNSKNDLELYRAVSLWCVSRLKFQPTS
                     GRDQAPLDITQKSYLGRCEEMQILFISAARTVGLPARPSYVPWWSHTDNNHAWAEVWI
                     DGAWHYTGDMDAAYYPDQTWFSGLIDKTVLILSKGTLPDATDEVLIQGRYECVINSTS
                     IYAKEHTRILKIKTLDEQDNPLPETTVGLLVFNSGTLRSLIYIQTDDEGEFELSVGRG
                     AFYLVADNENKRAMALVPSSEDKELYYELTLQEGPLPDQDEMLFYPASDFTWKNPSEE
                     WNNGVEQAKAKWNRREQRFQDRIFTYSDTLLGIVASSSRGNYNSFKGFWYRYPEPQKE
                     FLNFLISEDFDPKFLWQASSEQIIALYNFFLKIDGQNMTSSELAPILAPTVFYEELPQ
                     PVTYKKGIPQLYPDSFYQKGNTPLERMNSASGWLAKHYKINSSKALSGLLPLDIAIKQ
                     KYLTPLQYRMMAVNIARANKVPAYFSRQPDLIFIQFDNGDWGYYDLKKNAPQVDSKDS
                     TAYTKLIVSLSDENGVPVTGLSDAVNLTRYQDGVFYWLDQVFSEDEPGLISISVPKDS
                     YYLQAGYRVSDSQTAFQMKYLDLSETDSLFINIICRDYPRQWSENLPPELLSILEEID
                     SSRYQVILIGNYDQENSIRLAEKIRSSGKNFLWLGYEPSPQSIENYTFSRKWQMLVNL
                     EAQNRFRTITLFKQDGIWQYFEGMWERLPD"
     gene            complement(2931..5690)
                     /locus_tag="PK015_04640"
     CDS             complement(2931..5690)
                     /locus_tag="PK015_04640"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOL33787.1"
                     /translation="MNRRFLFLTILFIPFLLTATELTLNINFSDLELFSPQENYGWGT
                     LSVPGTPQLPTKNLNIILPPDAINVSYNYEFAPLQNLSAPAPVINTPFSDGENFLSTC
                     EHYPSTPMLKYLGIKHWGDIKFVSFSVLPAIYNGVSWQISPSLRINLSWSLNAISKPN
                     RIPPILSSLQKSNQVIDWFFVNPQDLQSYYTYNYSKDYDYLIVTTPTLYPSLAALEAY
                     HQSQGLVTSYININTILASSPGLTDGEKLRNYLISQYNAHPFTYLLLVGDYDKVPVMY
                     LTPEPDGNETVPSDFFYSDLSSIIDVDSDGRLGEYSSGEGDQDYLCDFTPELFVGRIS
                     NDNVNYISQIVNRTISFDSSDAVWKKKALLPAAYLNYAGEPETIFLETDGAVFMEYAR
                     NTALNDYQCITMYEHMGYLPSLPSDLDLDYDLLKNQLNSNSYGILNWSAHGSSVSSAR
                     KVWMNDDNGNNIPDSWEMNWYSLVDRETFDNLVNQDGLILFAASCNNGMLDNNFSCLA
                     EYALQQKAVNVCAATRTGWYKIGWKNPGWGGLSSYNYHFLENLTKNNMSVGAALAFAN
                     LLHTQYYLFGDPVDSDGIIYPELQNVYTYMLFGDPAVYHQNNQNPPLGEILVYEPLSQ
                     EGLPVVNALNNLGNFNVIYSDKLIPDYNYINNFEAIFCLFGWGPNNFIPEQGSLEYNL
                     LNDYLENGGRIYLEGDMPWDSDSIFWSKFGTICPLNTLAYIEAIRYDKDDLSMTWQYN
                     QNDPWTTPLFPYPETASELFYTSNSSHPNFPIGIYNSNGYFRTVASSFALSAIEEGEF
                     SINDMIAIILDTLNVLNLPPTPISDSVLTPSISKVITMPNPFSLQMSFCFETKEPSKT
                     QIQIFNLRGQKIKTIQSEALGAGKQNIIWDGCDAGGKPVPAGIYLWKMDNSQSIQQGK
                     LLKLH"
     gene            complement(5683..6495)
                     /locus_tag="PK015_04645"
     CDS             complement(5683..6495)
                     /locus_tag="PK015_04645"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOL33788.1"
                     /translation="MTLLLARTKLTPRQAEIFSYCESYFFAHENPGLAYKNSRYNLEG
                     YDAFGLAEYQIKELRNHILTIFQPSITELAELAYPFFATGKYEFGTLAIFLLKKHRPR
                     FNRRVYEEIKLCLDEVVENWTHSDLIATKITPVLLELEIASMEDFATWLDSESKWTRR
                     VAVVTMLYQKERLPVQELLDFISPLMQDQARCVQQGIGWLLRELSELYPQEIEEFLTQ
                     YKDSAAPMLIQYATGKNYKEKKNNFHKPYVKKGYQSHPNYHKKKRYKETDDE"
     gene            complement(6534..7301)
                     /locus_tag="PK015_04650"
     CDS             complement(6534..7301)
                     /locus_tag="PK015_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOL33789.1"
                     /translation="MIHIILVEPIYEGNVGAIARIMHNLGFSSLRLVGAMPKKNDFYL
                     AMHSENLLQQAQIYPTLKDAISDLDRVIAYSRRMGKLKPVDFTPITAAQYCAKIPQLE
                     IGLVFGRETFGLTDEEAALCPLRCHIPANPEFPSINLAQAVTIALWEIYRLPLTNEQT
                     PKINAVSGNDLDKIKAYMLRVMHNTGFFQRKETINWDNFLSKMLAQLNPTKIMVYRLR
                     QMFNRWNVLITGKGLGYEKNELNPAHKESNKYKEIQN"
     gene            complement(7298..8122)
                     /locus_tag="PK015_04655"
     CDS             complement(7298..8122)
                     /locus_tag="PK015_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOL33790.1"
                     /translation="MIKKVGRLDRDDYQLLDSYVEKISKNLDKGNINQAQKLIYEVAN
                     TPNYFVREELGKRLAEYTGTGNLDAICYEMLEDRFYPIRATALFYFYYKNQDKPELIV
                     QTLEKTFETVPWESETICSEMWKKYPDVMKEYMPRWADSDNPKKRAMSMHGMEYIAAK
                     NPQYVLTFVGRLLDDEDEEVQKKVSHILIQVGRQRPLQTFASIRRWLLDGDEKRANFL
                     WQILRRLSSIFSQKNMRDKSQDFFSIALRTAQEWKTDSNPAVVQMGNKLAKMIKNQ"
     gene            complement(8253..9203)
                     /gene="rnhC"
                     /locus_tag="PK015_04660"
     CDS             complement(8253..9203)
                     /gene="rnhC"
                     /locus_tag="PK015_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HOL33791.1"
                     /translation="MEKIIEEYLANFIPELAQRGIRITGQRGINYGIQLNLSKEEQHS
                     VLNIYYSEKRGLSKVLGGKDNKLKTELQEMIKDKVEPEGRYHSWEHWIGSDECGKGDY
                     FGALVVAAFALDSDMLEDLTDWGVMDSKSLTKTQITNIAQKLYSNYPSRISCIVLKPL
                     KYNEIIADMQLQGKNLNDLLAWQHSTAILALLERIPNIQGILVDQFSRSKKVANYLKK
                     RDVSIPVEERPKAETDPAVAAASIIARYQFLQQREAMNNYYGMKFPLGSGKNVIETAK
                     NFADKYGWNRLAEVAKLHFITSRSVRQRTIFDNSTDLTHK"
     gene            9387..11012
                     /gene="groL"
                     /locus_tag="PK015_04665"
     CDS             9387..11012
                     /gene="groL"
                     /locus_tag="PK015_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585059.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOL33792.1"
                     /translation="MAKQMLYGHEARTALKRGVDQLADAVKITLGPKGRNVVLDKKFG
                     SPLITNDGVTIAKEIELEDEFENMGAQLCKEVAEKTHDIAGDGTTTATLLAQAIIEEG
                     LKHVTAGVNAMYLRRGLEKATKVVVDKIHEYSKSINKSEEIAQIASISANNDYEIGKL
                     IAEAMEAVGKEGIINIEEAKSIDTGLEKVEGMQFDRGYISPYFVTNPDKMITEYEDCL
                     ILVHDKKISVLKDILPILQEVSQMGRPLLIIAEDIEGEALATLVVNKLRGVLNVVAVK
                     APGFGDRRKEMLGDIAILTGANVISEEMGRRLDSATMADLGRAKKVIVDKENTTIREG
                     AGDPEAIQARIKQIKAQIEETTSDYDKEKLQERLAKLSSGVAVIRIGAATETEMKEKK
                     ARVDDALHATRAAVEEGIVPGGGVTLIHAAKALQELKDLTYEEKVAVEILTKALEKPA
                     YQIAANAGAEGAVLVEKLKGYTDINMGFNAANGKFEDLLKAGIIDPAKVVRSAVQNAS
                     SIAGLLLTTECIVTDIKEPEKPAPMPTGMEGMY"
     gene            11652..14915
                     /locus_tag="PK015_04670"
     CDS             11652..14915
                     /locus_tag="PK015_04670"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOL33793.1"
                     /translation="MKLRYFKLTLLLILAFSVIGLGAQVSEYAFSSVLGTYTEITGGT
                     VLGTTANDQESFNAIPLGFTFTYNGVEYNQISVQTNAFIAFGPEVALSNLPISAATGT
                     NNVVAALARDIKSRDNGELSYLLTGTEPNRIFIIQWKHYRRVPTTAANDDLNFQIQLW
                     ENGNKVVFAYGTFNVVNISTAKTVQVGLRGDSAQDFNNRTTTTDWTATTAGTINTATC
                     DITNTVYPPNGLIFTFSPPQQGTPPEPAQNPHPSNNAINVPINSNLSWSSGGGIVDGY
                     KIYFGTDNPPTNLVNGLIQTTTVYDHPTDLQYSTVYYWKIVPFNNVAGDALNCPVWQF
                     TTLADPTVYNFPYNQHFDDVTPPALPVGWSTINANNDAYTWETYAGNAHTAPNSVRIR
                     YNSSIAMDDWLISPPLHLTGGEIYIIKFYYRANSSSYTEALSVYSGTAPDVAAFTNLL
                     FENNNISNIVYEQGQIVFTPATTGNYYIGFHGHSPANTFYLYLDTFSIVLNVDSMNPP
                     TNLTAEVINNTYVFLSWNAPSNTLNRDLLGYKVYRDGVLLQTINDPATTFYNDLNVTV
                     GTYSYTVTALYTAGESIPAGPVSATITPPAYPPTNLTAQVQGFNVILNWQSPALTLVG
                     DNKLDSSYRDRNLTGYKVYRNGTVVTTITNPSTTTYTDQNLTSGTYSYTVTAVYTYGE
                     SAPAGPVSVTIIPPPPTDLTATVQQNNVTLNWVSPQLPTELFIKAGSSTQDRALTGFK
                     VYRDNVIIATITNPLTTTYTDQNVPNGTYIYGVTAVYPNDESAPVTVTVTVDYQEPQI
                     VFEDGFETYENFALVFPPWSLYDVDQSGTYGINNVIFPGSESPMAFIIFNPSATTPPM
                     TSVAPHSGSKMAASFAAVNPPNNDWMIAPKVHLGTASSVKFFAKSQTDTYGLERFQVG
                     ISTSPNIDLSTFQIITTGNYVEAPIQWTEYNFDLSSYNNQEIWIGIHCVSNDAFIFYV
                     DDFCILSIGGYIVSNDDPTVPVLNTGLYGNFPNPFNPETTIRFSVKETAPVSITIYNA
                     KGQLIRTLVNNEIKTPGNYSIVWDGKDKNGNTMSSGVYFYKMNSGKYSDTKKMILMK"
     gene            15572..15647
                     /locus_tag="PK015_04675"
     tRNA            15572..15647
                     /locus_tag="PK015_04675"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15605..15607,aa:Ile,seq:cat)
     gene            complement(15663..16574)
                     /locus_tag="PK015_04680"
     CDS             complement(15663..16574)
                     /locus_tag="PK015_04680"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33794.1"
                     /translation="MIKAVIDLGTNSIKCIIAEIDNNSIRILKQVTFITKLGRNLQKN
                     DELGLEVQEDAIEALKSIQQLCNTLKVEQVVCAGTEALRRAKDSASFLKKIKQQFGWQ
                     VYVLSPEMEAKLIFLASSNLIPAGEKAMLVESGGGSTEFIFGYRERLLSYNFFPFGAL
                     TLTNKFVHNDPISPEEFNTLKDFLTQKLEQTFPEPKNLFSLASGGGATTLASVSLKLE
                     HFEDEAIHNYFLASEEIKRQVELYKTLNSEQRRKIKGMQPGREDIILASAVIYQQIAT
                     HFNLPGFIISSRGIRYALLQYELKSLN"
     gene            complement(16567..18855)
                     /locus_tag="PK015_04685"
     CDS             complement(16567..18855)
                     /locus_tag="PK015_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOL33795.1"
                     /translation="MNKSYPHISGKLHLTGSSHFIADEIPLPEMLYAKFLFSPVAHAN
                     ITELDISEASAFPGVEKVITAADIPGINQIGLVIQDEPLFPEKEIMYIGQPLAMVLAQ
                     DEKIAEAAVKLIHLKYEELPPIFEIEEADEKGEWYVPERKIESGDVQKALTESEFILQ
                     GITETPTQEHFYLETQRCRAIPGEDGQIFLHTATQSTMEVQEVVSRLLNLPANHIIVE
                     VPRLGGAFGGKERGGTIWATMAALGCFLTHKPVQVLLEREEDMRSTGKRHPFQGRWKV
                     GFDAQGKIEALDIELLSNGGAYTDLSIAILERAMFHAENAYFIPNVRIRGRACRTNLP
                     PNTAFRGFGAPQAIYIIEYIMEQIAQKLQLDPLKLRELNAYRSGDITPYGQKVMECNF
                     PAIFERLAKNSNYLQLKEKVEQYNALHKDRKQGLGIVPVKFGISFTTTHMNQASALLW
                     IYIDGTASLTTSAVEMGQEVSTKCATVVSEILGIPIENIRVESSNSQRVGNSSPTAAS
                     TGTDLNGNASRIAAEKIKSQLLPIAQEMLKNQGIDCKLEKIYFKDGTVYSSDYPEKKI
                     EFKDLIRQAYLSRIPLGAYGYYRTPSLSFDREKGKGSPFHYFVFGAALAQVQIDLLTG
                     EHQILKVYIVHETGNSLNKDIDRGQIEGAFIQSMGWCTCEELPRNDKGHYLAITPSTY
                     KIPTVRDLPLNIQIEMISSGSQYASIFGSKAIGEPPFIYGLTVWFAIQNAIKSFVPQA
                     LLKFPATPEAILLALQREKEFD"
     gene            complement(18848..>19161)
                     /locus_tag="PK015_04690"
     CDS             complement(18848..>19161)
                     /locus_tag="PK015_04690"
                     /inference="COORDINATES: protein motif:HMM:NF015415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="xanthine dehydrogenase"
                     /protein_id="HOL33796.1"
                     /translation="KRKAVDISSVVSAIKIVSEAGIIQEAQLAFGGVAPIPKLSSKFP
                     ELIIGKSKEEINPLEFAELIADEFNPISDVRGSKEYRQLVIRNQVLNYLQEFLEGGFN
                     E"
     assembly_gap    19162..19261
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<19262..20505)
                     /locus_tag="PK015_04695"
     CDS             complement(<19262..20505)
                     /locus_tag="PK015_04695"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOL33797.1"
                     /translation="MEVHNSENLIKLLQRTYNFNKIQFWLNGKPITAEIPPAQTTLQF
                     LHTNQHLYGTKCSCNEGDCGACTVVIAYVKEGNIVYEAINSCLYNAAKLHGKHLITIE
                     GLGTPEHLHPIQEVMYKQHSLQCGYCSPGFVMSLFALLANSSHPDEEEILASLEGNLC
                     RCGSYQSILRATRELSENFTTSDIIPDWCRAIEPRLFCFKDKSETVQRSGKLLFPCTS
                     YLIPRSLSELTDILKNEPEAVFITGGTDVMVRMNIQKEEIPVLIDLSELPELKQINYT
                     KQGIIIGAGVTYSDIQHSGIIKSDLPSLYELVNKIASRQIRNFATLAGNIANASPIGD
                     TLPLLLVMDASLTLLSSCGIREIPLQDFFLDYRKTKLQPKEIIKEIIIPVPAKDAFIR
                     TTKATKRKAVDISSVVSAIKIVS"
     gene            complement(20645..>21216)
                     /locus_tag="PK015_04700"
     CDS             complement(20645..>21216)
                     /locus_tag="PK015_04700"
                     /inference="COORDINATES: protein motif:HMM:NF021622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33798.1"
                     /translation="ATEIAKLLLLNFHPDLSSGTNHILALMFDMNKLWEKFIFITLRK
                     QFIRNQSKYNVYAQRKKLFWSSEYNKREIKPDIIIQEQTNEANKQTDCYILDTKWKVP
                     ENINAIPSDSDLMQIFAYANIFNTHKNALVYPGNPKNSKKGHYESPEDTQKETIGCDT
                     ILLECKENIKEFQESIYSTISEWLSQKME"
BASE COUNT         6542 a         3805 c         3987 g         6782 t
ORIGIN      
        1 taaccactaa agaaggaaaa gccttacggc tttgcgagat gaggacatct cgcaccccat
       61 agtggggtca taaactttct cttacacggg gtcataaacg tccccgttta tgaagctccg
      121 aaggagcttg ttgttaataa tgtattaaaa actaagattt atattctttg tttatataac
      181 cactaaagaa ggaaaagcct tacggctttg cgagatgagg acatctcgca cccaatatga
      241 ggagctttct tatttaaaga gagaataatg agagatttat agataattta gaaaagcctt
      301 actgatttgc gagatgagga catttcgcac tccataaggt gatatctttc cctcctgaat
      361 gaattatatt aatctggtaa acgctcccac atcccttcaa aatattgcca gataccatct
      421 tgtttaaata gggtaatagt tctgaagcga ttttgtgctt ccagattaac cagcatttgc
      481 cattttctac taaaggtata attttcaata gattgaggag atggttcata acccagccag
      541 aggaaatttt tacctgaaga acgaattttt tctgccagac ggatactgtt ttcctggtca
      601 taattgccta taagaataac ctgatatctt gagctatcta tttcttctaa aatagatagg
      661 agttcaggag gtagattttc actccactga cgaggataat cgcgacaaat aatatttata
      721 aaaagactat cggtctcaga caagtccaga tatttcattt gaaaagcagt ctgactatcg
      781 gaaaccctgt atcctgcttg taggtaataa ctatctttgg gaacgcttat agatattaaa
      841 ccaggctcat cttccgaaaa aacttgatcc agccagtaaa aaactccatc ctgataacgg
      901 gttagattaa ctgcatcaga gagtccagtt acaggaactc cattttcatc tgaaagagag
      961 acaatcaatt ttgtataagc ggtggaatct ttactatcta cttgaggagc attctttttc
     1021 aaatcataat aaccccagtc cccgttgtca aactggataa atatgagatc gggttgacga
     1081 gaaaaatagg caggaacttt gttagctctt gcaatgttta cagccatcat tctgtattgc
     1141 agaggagtca ggtatttttg tttgatagca atatccagtg gaagcaatcc acttaaagct
     1201 ttagaagagt taattttgta gtgtttagcc agccacccag aagcagaatt cattctttcc
     1261 agaggtgtat tccctttttg gtagaaagaa tcaggataga gttgagggat tcctttttta
     1321 taagtaacag gttgagggag ctcttcataa aatactgttg gtgcaagaat aggagctaat
     1381 tctgaagagg tcatattttg accatctatc tttaagaaaa agttgtagag agcaataatt
     1441 tgctcggaac tggcttgcca gaggaattta ggatcaaagt cttcagaaat aaggaaatta
     1501 aggaattctt tttgaggttc aggatagcga taccagaacc ctttaaagga attataatta
     1561 cctcttgaag aggatgcgac tattcctaaa agtgtatcac tataggtgaa aatccgatct
     1621 tgaaaacgct gttcccggcg attccatttt gctttggctt gttccacacc attattccac
     1681 tcttcagaag gatttttcca ggtaaaatca gatgcaggat agaagagcat ttcatcttga
     1741 tctggtaggg gtccttcttg caaagtaagt tcataataaa gttctttatc ctcactggaa
     1801 ggaactaacg ccatagcacg cttattttca ttgtctgcca ccagataaaa agcacccctg
     1861 cctacactta gctcaaattc cccttcatca tccgtctgaa tataaatcaa agaccttaaa
     1921 gttccagaat taaaaaccag cagaccgaca gttgtttcag gaagaggatt atcctgctca
     1981 tccaaggtct tgattttgag aatacgagtg tgttctttag cataaattga agtggaattt
     2041 atcacacatt cgtagcgtcc ttgaattaaa acttcgtcag ttgcatcagg aagagttcct
     2101 ttagatagga taagtacagt tttatcaatc aatccactga accaggtttg atcgggataa
     2161 taagcagcat ccatatctcc agtataatgc caggctccat caatccagac ttctgcccaa
     2221 gcatgattat tatctgtatg agaccaccag ggcacataag aaggacgagc tggcaatcct
     2281 accgttcttg ctgcagagat aaacaagatt tgcatctctt cgcatcttcc cagatagctc
     2341 ttttgagtta tatccaaagg tgcctggtct cttcctgaag tgggctggaa ttttaaacgt
     2401 gagacgcacc ataatgaaac ggcacgataa agttccaggt catttttaga gttctgtagc
     2461 acctctcgta agccatccct aagaaattct gctctataag gtgtgattct ctcatctgca
     2521 acagtttgat gagcaacata ggaaaggaaa aattcaggag aaaggtcagt cccataagta
     2581 ttgagatagg accggatttg taaataatta cttaggacat cttctggatt tgctatttgt
     2641 aagatagcat cgctctcgta ggcaataaga taggacatca atatatcatc ctgttctttc
     2701 aataatttct tataggcttg ctgatgaaaa tggtctaatt ttttgagatt agaataagca
     2761 agtttattca cttttttata aaggacagga tttccttgat tggtgaaatt agcaggtaaa
     2821 tatgaggcaa gaaggcttga aaaaccccac accatacagc aaataattat tagtatctta
     2881 atttttttca tatgtgacct atctatttat gttctttaaa gcagatttca tcagtgaagc
     2941 ttgagtaatt ttccctgttg aatgctttga gaattatcca tcttccagag ataaatacct
     3001 gcaggaacag gttttccccc ggcatcacag ccatcccaga taatattttg ttttcctgcc
     3061 cccaaagctt cagactgtat ggttttaatc ttttgaccgc gcagattaaa tatctgtatt
     3121 tgagttttag agggttcttt agtttcaaaa caaaagctca tttgcaaaga aaaaggattg
     3181 ggcatcgtaa tcaccttaga aatggaagga gtaagaacag aatcagaaat gggagtaggt
     3241 ggtaaattca gaacatttag agtatccaga ataattgcta tcatatcatt aatgctgaat
     3301 tctccttctt caatagcgga aagggcaaaa gaagaagcaa ccgtgcgaaa atatccattg
     3361 ctattataga ttccgatagg gaaattggga tgagagctat tagaagtata aaatagttca
     3421 gaggcagttt caggataagg aaataaagga gtagtccagg ggtcattttg attgtattgc
     3481 caggtcatag aaaggtcgtc tttatcatat ctgatggctt caatataggc aagagtatta
     3541 agcggacaaa tagttccaaa ctttgaccag aagatggaat cagaatccca aggcatatct
     3601 ccttccagat agattcttcc accattttcc agataatcat tgagcaggtt atattccagg
     3661 cttccttgct caggaataaa attgttaggt ccccagccaa aaaggcaaaa gatagcttca
     3721 aaattgttta tatagttata atctgggatg agtttatcgg aatagattac attgaagttt
     3781 cccagattat tcaaagcatt aactactggt aaaccttctt gagaaagcgg ttcataaacc
     3841 agtatctctc ctaatggagg attttgatta ttttgatggt aaacagcagg gtctccaaaa
     3901 agcatatagg tataaacatt ttgtagttct gggtagatta tgccatcact atccaccggg
     3961 tcaccaaaga gataatattg cgtatgtaat agattggcaa aggcaagagc agcaccaacg
     4021 gacatattgt ttttggttag attttccaga aaatgataat tataggagct caaacctccc
     4081 caaccaggat ttttccagcc tatcttatac caaccagtac gcgtggcagc acagacatta
     4141 acggcttttt gctgtagagc atattcagct aaacaagaga agttattatc cagcatacca
     4201 ttattgcagg aagcggcaaa aagaattaat ccatcctggt tgaccaaatt atcaaaagtc
     4261 tcacgatcaa ccagactata ccagttcatt tcccaactat ctgggatatt attaccatta
     4321 tcatcattca tccaaacctt tcgggcagaa ctgacagaag aaccatgtgc actccaattc
     4381 aggatgccat aactattgct attcaattga tttttcagga ggtcataatc caaatcaagg
     4441 tcagatggta gagaaggtaa atatcccata tgttcgtaca ttgtaataca ttgataatca
     4501 tttaaagcag tgtttctggc atattccata aaaactgcac catctgtttc cagaaaaatt
     4561 gtttcaggtt ccccggcata attaagataa gcagcaggca ataaagcttt cttcttccag
     4621 actgcatcac tactgtcaaa gctaatagtg cgatttacaa tctgtgaaat atagttaaca
     4681 ttatcgtttg aaatgcgtcc cacaaagagc tcaggagtga aatcacagag ataatcctgg
     4741 tctccttctc cagaagaata ttctcccaga cggccatcac tatccacatc tatgatactg
     4801 cttaaatcgc tgtaaaagaa atcagagggg acggtttcat taccatctgg ttctggagtg
     4861 agatacatca cgggaacttt atcataatct ccaactaata aaagataggt gaaaggatga
     4921 gcattgtatt gggagattag ataatttcgg agtttttcac catctgtaag tcctggactt
     4981 gaagctaaaa tagtattgat attgatataa gaagtgacca gaccctgtga ctgatgataa
     5041 gcttccagag cagctaagga aggatataaa gttggtgtgg taactattaa ataatcataa
     5101 tctttagaat aattataagt gtaataactt tgtaaatctt gaggattaac aaaaaaccag
     5161 tctattactt gattgctttt ctgtaaagaa ctaagaatgg gaggtattct atttggtttt
     5221 gagatagcgt ttaagctcca ggaaagattt atccgtaaag aaggactaat ttgccaggaa
     5281 actccattat agatagcagg taaaacagaa aaagaaacaa attttatatc accccagtgt
     5341 ttaattccca agtattttag cataggcgtt gagggataat gttcacaagt agaaaggaaa
     5401 ttctctccgt cactaaaagg agtattgata acaggagcag gtgcagacaa attctgcaaa
     5461 ggagcaaatt catagttata ggagacattt attgcatctg ggggaaggat gatatttaag
     5521 tttttagttg gcaattgagg tgttcctgga acagataaag ttccccaacc ataattttct
     5581 tgtggagaaa agagctccag gtcagaaaaa tttatgttta atgttagctc tgttgctgta
     5641 agcagaaaag gaataaacaa gatagttaaa aataaaaatc ttctattcat cgtcggtttc
     5701 cttatatctt tttttcttgt ggtaattggg atgggattga taccctttct taacatatgg
     5761 tttatgaaag ttatttttct tttctttata gttctttcct gtagcatatt ggattagcat
     5821 aggagcagca gaatctttgt attgagtgag aaactcttct atttcttgag gatacaactc
     5881 gctaagttca cgcaataacc aaccaattcc ctgttgaaca caacgagctt gatcctgcat
     5941 aaggggactg atgaaatcca ataattcttg gactggtaag cgttcctttt gatagagcat
     6001 agtgacaacc gctacccgcc tggtccattt gctttccgaa tccagccagg tggcaaaatc
     6061 ttccattgaa gctatttcca gttctaaaag cacgggagta attttagtgg caattaaatc
     6121 agaatgagtc caattttcta ccacttcatc cagacaaagt ttaatctctt catagactct
     6181 acggttaaag cgaggtctat gcttctttaa caaaaaaata gctaaggttc cgaattcata
     6241 ttttccagtt gcaaagaagg gatatgccag ctcggctaat tccgtaatag atggttggaa
     6301 aatggttaat atgtgattgc gtaactcttt aatttgatat tctgctaaac caaaagcatc
     6361 ataaccttca agattatagc gagaattttt ataggctaat ccagggtttt catgggcaaa
     6421 gaaatatgat tcacagtaac taaaaatctc tgcctgtcgt ggagtgagtt ttgttcgagc
     6481 taaaagtagt gtcatttgaa tctctgtttt ttaaaatcca gaattattaa aaattagttt
     6541 tgtatttcct tatatttatt agactcttta tgagctgggt ttaattcatt tttttcatat
     6601 cccagaccct taccggtgat aagtacattc catctattaa acatttgtct taagcgatat
     6661 accattattt tggtagggtt taactgtgcc agcattttac ttaagaaatt atcccaattt
     6721 atagtttctt tgcgctggaa aaatcctgtg ttatgcatta ctcttagcat ataagccttg
     6781 attttgtcaa ggtcatttcc gctaactgca ttaatttttg gggtttgttc attagtgaga
     6841 ggcaaacgat atatttccca gagagcgata gtgacagctt gagcaagatt gatagaaggg
     6901 aattctggat tagcaggaat atgacaacgc aaagggcata gtgctgcttc ttcatccgtg
     6961 agtccaaaag tttctctacc gaagactaaa cctatttcca gttgagggat tttagcacaa
     7021 tattgagcag cagtaatagg tgtgaaatct actggtttga gttttcccat acgacgagaa
     7081 taggctatta cccggtctaa atctgagata gcatccttta gagtgggata tatctgagcc
     7141 tgttgaagta ggttttctga atgcatagcc agatagaaat catttttttt cggcatagca
     7201 cctactaacc ttagactgga gaagccaaga ttgtgcatta ttcgtgctat agcacctaca
     7261 ttcccttcat agataggctc aactaagatt atgtgaatca ttggttttta atcattttag
     7321 ctaatttatt tcccatctga accactgcag gattggaatc agttttccat tcttgtgcgg
     7381 ttctaagcgc tatggaaaag aaatcttgag acttatctcg catatttttt tgagagaaga
     7441 tactggataa tcttcttaga atctgccaga ggaaattagc acgcttttca tcaccatcca
     7501 aaagccagcg tcggatactg gcaaaggttt gtaggggacg ctgtcttcca acttgaatga
     7561 ggatatgact aacttttttt tgaacttcct cgtcctcatc atccagcaat ctacctacga
     7621 aagttaatac atattgagga tttttagcgg ctatatattc cataccatgc atagacatag
     7681 cgcgtttttt aggattatct gaatcagccc agcgaggcat atattctttc atgacatcag
     7741 gatatttttt ccacatctct gaacagatag tctcgctttc ccaaggaacg gtttcaaagg
     7801 ttttttctaa ggtctggaca atgagttctg gtttgtcctg atttttgtaa taaaaataga
     7861 ataatgcagt tgcccggata ggataaaatc gatcttccag catctcatag caaattgcat
     7921 ccaagtttcc tgtgcctgta tattccgcta aacgttttcc caattcttcc cggacaaaat
     7981 agttaggagt attagcaact tcataaatca gcttttgcgc ctgattaatg tttcccttgt
     8041 ccagattttt ggaaatcttt tctacataag agtccaataa ttggtaatca tcgcggtcca
     8101 ggcgtcctac tttttttatc atatttttca gctccataat ccctatgagg gatctatccg
     8161 cggaattctt aaaattagta agcctgttgt cctacgagaa aatagattat taaatatatt
     8221 acctaagata taaaattatc gtttctgtca agttatttgt gggttaaatc ggtagaatta
     8281 tcaaaaatag tgcgttgtcg cactgaccga ctggttataa aatggagttt ggctacttct
     8341 gctaaccgat tccaaccata tttatcagca aagtttttag cagtttcaat aacatttttc
     8401 ccggaaccca gaggaaactt cataccataa taattattca ttgcttctct ttgttgtaaa
     8461 aactgatacc gagctattat tgacgctgca gcaacggctg ggtctgtttc tgctttaggt
     8521 ctctcttcca caggaatact aacatctcgt tttttaagat aattagcaac ttttttactg
     8581 cggctaaact ggtcaactaa aataccctgg atattgggaa ttctttctaa tagagcaaga
     8641 atagcagtgc tatgttgcca agccagaagg tcattaaggt tcttcccttg caactgcata
     8701 tctgctatga tttcattata ttttaaaggt ttgagcacga tgcaagaaat gcgagaagga
     8761 tagttagaat aaagtttttg agctatgttt gtaatttgag ttttagtcag acttttactg
     8821 tccatcactc cccaatctgt taggtcttca agcatatcac tatccaaagc aaaagcagca
     8881 accaccaggg cgccaaaata atcacccttt ccacattcat cagaaccaat ccaatgctcc
     8941 caggaatgat atcttccctc gggttctact ttatctttta tcatttcttg aagctctgtt
     9001 ttcaatttat tatctttacc accaagcacc ttactcaaac ctcttttttc agaataataa
     9061 atatttagta cggaatgctg ctcttcttta cttaaattaa gttgaattcc ataatttatt
     9121 cctctttgac cagtaattct gattcctctt tgagctaatt caggtataaa attagccaga
     9181 tattcttcta taattttttc cataaactac cttagatatt attgtaatat ccttttctaa
     9241 ggaatagaca gagatgtcaa tctcaaaatt aagaaaaaaa taatttgtgc ttgacagaat
     9301 ttggtttatc ttaattttag cactcaatca ttgagactgc taaaccttaa tttaataaaa
     9361 tatgataata tggaggtata aagaaaatgg caaaacaaat gttgtatgga catgaagccc
     9421 ggactgcttt aaaaaggggc gtagatcagc ttgctgatgc agttaagata actctcggtc
     9481 ccaagggtag aaatgtggtt ttggacaaaa aatttggttc ccccctaatc actaatgatg
     9541 gagtaacaat agcaaaagag attgagttgg aagatgaatt tgagaatatg ggagctcagc
     9601 tttgcaaaga agtggcagaa aagacccatg atattgctgg agatgggact acgacagcaa
     9661 cccttttagc tcaggctatt attgaagaag gattaaagca tgtaactgct ggggttaatg
     9721 caatgtatct tagacgaggc ttggaaaaag ctactaaggt cgttgtggat aagatacacg
     9781 aatattccaa atcaatcaat aaaagtgaag agatagctca aatagcttct atttcagcca
     9841 acaacgatta tgaaattggt aaattgattg cagaagctat ggaagcggtt ggaaaagaag
     9901 gtattatcaa tatagaagaa gccaagtcta tagatactgg tctggaaaaa gttgaaggaa
     9961 tgcagtttga tcgtggctat atttctcctt actttgtcac caatcctgat aaaatgataa
    10021 ctgaatatga ggattgttta attcttgtcc acgataagaa gataagtgtt ctaaaagata
    10081 ttttgcccat attgcaggaa gtttcccaga tgggacgtcc cttattgatt attgctgaag
    10141 acattgaagg tgaagctctt gctactctgg tagtgaataa acttcgtgga gttctgaatg
    10201 tggtagcagt taaagctcct ggcttcggag atagacgcaa agaaatgctt ggggatatag
    10261 ctatactcac gggagcaaat gtaatttcag aagagatggg tcgtcgtttg gatagtgcta
    10321 cgatggctga tcttgggaga gccaaaaaag tgatagtgga caaagaaaat accactattc
    10381 gtgagggagc aggagatcct gaagcaattc aagccagaat aaaacagatt aaagctcaaa
    10441 ttgaagagac cacttcagat tatgacaaag aaaaactaca agagcgtctg gcaaaacttt
    10501 ccagtggtgt tgcagttatt cgtattggag ctgccaccga gacagaaatg aaggaaaaga
    10561 aagctcgtgt ggatgatgct cttcatgcta ccagagcagc tgtggaagaa ggaattgttc
    10621 ctggtggcgg tgtcactcta attcacgcag ctaaggctct tcaggaatta aaggatttaa
    10681 cctatgaaga gaaggtggca gtggagattc taactaaagc tctggaaaaa cctgcttatc
    10741 aaattgctgc taatgcagga gcggaaggag cagtactagt agaaaaactt aaaggttaca
    10801 cggatattaa tatgggattc aatgctgcta atggcaaatt tgaagacctg cttaaagctg
    10861 gtattattga cccagctaaa gtggttcgtt ccgcagttca aaatgcctct tcaatagctg
    10921 gtttattact gactacagaa tgcattgtaa ccgatattaa agaaccggaa aaacctgctc
    10981 caatgcctac tggtatggaa ggaatgtatt aataaactta gagaatagag catatctcaa
    11041 aagcctctcc cacaaagaga ggctttttag tttgccccca aattagatag aaagatagca
    11101 gtataaacaa agcaaatatt aaatcattat ctaccacttt actggtgaat aactacaaaa
    11161 tcattcctga gcttctgaat aaattcatcc agaagtgagt tcgtgtctca aatagtggtg
    11221 taaaattcaa cttagtgttt ataatttatt gaggaacatc ttatattttt acctttactt
    11281 cctattttga gataattagt aacatccaaa tgtgtcggta ttggtacatt gagtatttat
    11341 atattatcag atatggtcag tatttcaccc catatccgat ttactgtaag cgaggcttct
    11401 ttcagagatg attttttgga ttccaggtaa gtgtgctttg cgatgtgtat aattatagac
    11461 ttatactacc acttttaact atcagtgaga ccaaagattt ttatttaaag ctattaatat
    11521 aggtaatcag atttttcttg accaaattat tagtttttat ttagataatt tttataattt
    11581 aaaatgatgg aatataaaat gctgttataa tgaataacag aattatcata taacattata
    11641 aaggagatat gatgaaatta agatatttca aacttacact tttactgata cttgcatttt
    11701 cagtaattgg tttaggagct caggttagcg aatatgcttt ttcttcagtt ttaggaactt
    11761 acacagagat tactggagga actgttctgg gcactactgc aaacgatcaa gaaagcttta
    11821 atgccattcc cttaggtttt acttttactt ataatggtgt tgaatacaac caaatcagtg
    11881 ttcaaactaa tgcctttatt gcctttggac cagaagttgc tttaagtaat cttcccataa
    11941 gtgctgccac aggcactaat aatgtagtcg ctgcattagc acgggatatt aaaagtagag
    12001 ataatggtga attaagttat ttactaactg gaacagaacc taatcgtatt tttattatcc
    12061 agtggaaaca ttatcgtaga gttcctacta ctgctgctaa tgatgattta aattttcaaa
    12121 ttcaactttg ggaaaacggt aataaagtgg tttttgctta tggcacattt aatgtcgtta
    12181 atattagtac tgctaaaact gtacaagtag gcttaagagg tgattctgcc caggatttta
    12241 ataatcgtac cactactact gattggacag ccactacagc aggaacaata aatactgcaa
    12301 cttgcgatat aacgaatact gtgtatccac ctaatggttt aattttcact ttttctcctc
    12361 ctcagcaagg tactcctcca gaaccagctc aaaatccaca cccctctaat aatgcaataa
    12421 atgtccctat taattctaat ttaagctgga gttccggagg tggcattgtg gatggttata
    12481 aaatctattt tggaacagat aatcctccta ccaatctggt taatggtttg attcaaacta
    12541 ccaccgtcta tgaccatcct acggatttac aatatagcac ggtctattac tggaaaatag
    12601 ttccttttaa taatgttgca ggtgatgctt taaactgtcc cgtatggcaa tttactactc
    12661 tggcagatcc aacagtttat aatttccctt ataatcaaca ttttgatgat gtgacccctc
    12721 cagctctgcc agttggatgg tcaactatca atgctaataa tgatgcctat acctgggaaa
    12781 cttatgcagg taatgcccac actgctccaa attctgttag aattcgttac aattcatcta
    12841 ttgcaatgga tgattggttg attagccctc ccttacattt aaccgggggt gaaatctata
    12901 taattaagtt ttattatcgt gctaatagta gttcttacac tgaagctttg tcagtttatt
    12961 ctggcacagc accggatgta gcagctttca ccaatctcct atttgaaaat aataatattt
    13021 ccaatatagt ttatgagcaa ggacagatag ttttcacacc agctacaacg ggtaattatt
    13081 atataggatt tcacggtcat agtccagcta atacgttcta tctgtatctt gatacttttt
    13141 ctatagtgtt aaatgttgat tcaatgaatc ctccgactaa tttgacagcc gaagtgatta
    13201 ataatactta tgtttttctt agctggaacg caccaagtaa tacattaaat cgtgatttat
    13261 tagggtataa agtatatcgt gatggtgttt tattacagac tataaatgat cctgctacaa
    13321 ctttctataa tgaccttaat gttaccgttg gaacttatag ttataccgtt actgctcttt
    13381 atactgcagg tgaatccata ccagcaggtc ctgtctctgc aacaattaca cctccagctt
    13441 atcctccaac taatttaact gcgcaagtgc aaggatttaa tgttattcta aactggcaaa
    13501 gtcctgcttt aactctggtg ggagataata agttggatag tagttataga gatcggaatt
    13561 taacgggata taaagtttat cgtaatggca ctgttgttac tacgataact aatccttcca
    13621 ctactactta tactgatcag aatctaactt ctggaactta ttcctacacg gtaacagcag
    13681 tatacactta tggtgaatct gcaccagctg gtcctgtcag tgtaactatt attcctccac
    13741 ctccaacaga tttaaccgca acagtgcagc agaataatgt gactttgaac tgggttagcc
    13801 ctcaacttcc cactgaatta ttcattaaag ctggttcatc aactcaagac agagcactta
    13861 ctggatttaa agtatatcgt gataatgtta taattgccac cataactaat cctttaacta
    13921 ccacctacac tgatcagaat gttccgaatg gcacttatat ttatggtgtt acagccgttt
    13981 atcccaatga tgaatctgct cctgtaaccg tgactgttac cgtggattat caagaaccgc
    14041 aaattgtatt tgaagatggt tttgaaacct atgagaattt tgccttagtc tttccaccct
    14101 ggtcccttta tgatgtagac caatctggaa cttatggtat taacaatgta atttttccgg
    14161 gatcagaaag tccaatggct ttcattattt tcaatcccag tgccacaaca cctcctatga
    14221 ccagtgtagc tccacatagt ggctcaaaaa tggctgcttc ttttgctgca gttaatcctc
    14281 ctaacaatga ttggatgatt gcacctaagg ttcatctggg aacagcaagt tcggtaaaat
    14341 tctttgctaa atcccaaact gacacttatg gtctggaacg tttccaggtg ggaatttcta
    14401 catcaccgaa tatagattta tccaccttcc agattattac tactggtaac tatgtggaag
    14461 ctcccattca atggactgaa tataattttg acctttcttc ctataataat caggaaatct
    14521 ggataggtat acactgtgtt tctaacgatg catttatatt ctatgttgat gacttctgta
    14581 tccttagtat tggtgggtat atagtcagca atgatgatcc aaccgttcca gttttaaata
    14641 ctggtttata tggtaacttc cccaatcctt tcaatcctga gactaccatt cgctttagtg
    14701 ttaaagaaac cgctccagtt tctataacca tttataacgc taaaggacaa ttgattagaa
    14761 ctctggttaa taatgaaatc aagactcctg gcaattattc tatcgtctgg gatggaaagg
    14821 ataaaaatgg gaatacaatg agcagtggag tctatttcta taagatgaat tcgggtaaat
    14881 atagcgacac taagaagatg attctgatga aataagatga aagaacaacc tcatacctga
    14941 ttggattatt tataaagaac tattcaggta ttgataaatg aaatgaaggg cacgaatttt
    15001 ctggattcgt gccctttagt tttctttgat aaatttcata gatggttata tcttagatta
    15061 gttaaaatcc tgcgtaaata tatccttgac aaaagagtta aactgaaatt aaaggaatct
    15121 tataaggtac tttagatgag taagtatata aaagcaattg acccgtttga gggggttgaa
    15181 acaaacttat tctctgggat acagatgagt ttctgattat taataggtga gggggattaa
    15241 gatactcatc ccccaggagt tagctgaatc taacttattg actccccctc atattttagc
    15301 agagacatat attcttctaa ggtgtaggga aaatcaagtt gagtaggtta agtgggtaat
    15361 ttctcaatgt tccaataagt aaaaaattgg gattttaaga gcttggttgg tgttttatct
    15421 tatctgtttc ctatctcttt cttattgaaa aagacaagta agagctaagg aagagctaag
    15481 agagagttaa aatatctttg taaataatgc acttagcgat tttgcttgac ataaaagtag
    15541 agatttaaat gaaagttaaa ttgaaaggtg ggggcctata gctcagctgg tagagcttcc
    15601 ggctcataac cggatggttg ggggttcaac tccctctggg cccaccattt tttatttatc
    15661 aattaattta aacttttaag ttcatattgt agcagggcat aacgaattcc acgagaagag
    15721 ataataaatc ctggaagatt aaagtgggtt gctatctgct gataaatgac agcactggca
    15781 aggataatgt cttctcgtcc gggctgcatt cctttaattt ttcggcgttg ctcactattc
    15841 aaagttttat atagttccac ttgtctttta atctcttctg aagcaagaaa atagttgtga
    15901 atagcctcgt cttcaaaatg ttctaatttt aaactcacag aagctaaagt ggttgctcca
    15961 ccaccactgg caagggagaa aagatttttt ggttcgggga aagtttgttc taacttttgc
    16021 gttagaaagt ctttaagagt attaaactct tctggtgaaa tagggtcgtt atgtacaaat
    16081 ttattcgtta aagttagtgc tccaaaggga aagaagttgt aggaaagtaa cctttcacgg
    16141 tatccaaaaa taaattctgt gctacctcca ccgctttcaa ccagcattgc tttttctcct
    16201 gctggtatga gattagaaga agcaagaaag ataagcttgg cttccatttc aggtgaaaga
    16261 acataaactt gccagccaaa ttgttgttta atctttttca gaaaggaagc agaatcttta
    16321 gctcttcgta atgcctcagt tccagcacat acaacttgct caactttaag tgtattacat
    16381 agttgctgga tagattttaa ggcttcaatg gcatcctctt gaacctctaa gcctaactca
    16441 tcgttttttt gcagattcct gcctaattta gtgatgaagg ttacctgttt taagatacgg
    16501 atactattat tatctatctc agcgataata catttaatgg agttggttcc taaatctatt
    16561 actgctttaa tcaaattcct tttctctttg taaagcaaga agaattgctt caggagtagc
    16621 aggaaacttt aataatgctt gagggacaaa actctttatg gcattttgga tggcaaacca
    16681 gacggttaga ccataaatga aaggaggttc cccaatagct ttgctaccaa aaatgctggc
    16741 atattgactt cctgaagaaa tcatttctat ctgtatatta agaggtaagt ctctgactgt
    16801 aggtatttta taggtggaag gagtgatagc aagataatgt cctttatcat ttcggggaag
    16861 ttcttcacag gtacaccatc ccatagactg aatgaaagcc ccttctatct gacctcggtc
    16921 aatatcttta tttaaactat ttccagtttc atggacaata tagactttca atatttggtg
    16981 ttctccggtt aataaatcaa tctgaacctg agctaaagca gctccaaaaa cgaagtaatg
    17041 aaaaggtgaa ccctttcctt tctcacggtc aaaagaaaga gaaggtgttc tatagtatcc
    17101 ataagctcct aagggaatac gtgaaagata agcctgacgg attaggtctt taaactcaat
    17161 ctttttctct ggataatcgg aagagtacac agttccatct ttaaagtaaa ttttctccag
    17221 tttgcaatca ataccttgat tcttaagcat ttcttgagct ataggaagca gttgggattt
    17281 aattttttca gctgctattc gagaagcatt tccattcaag tctgtaccag ttgatgcagc
    17341 agtaggagat gaatttccca ctctttggga attactgctt tctactcgga tattttcaat
    17401 tggtatgcca agtatttctg aaactaccgt ggcacattta gtgcttactt cctgtcccat
    17461 ttccactgca ctggtggtta aggatgcagt cccatcaata taaatccaaa gtaaagctga
    17521 agcttgattc atatgcgtgg tggtaaaaga tataccaaat ttaactggaa caatacccag
    17581 tccttgttta cggtctttat gtagggcatt atattgttct actttttctt ttagttgcag
    17641 ataattgctg tttttagcta atctttcaaa aatagcaggg aaattgcact ccataacttt
    17701 ttgtccataa ggagtgatat caccagagcg ataagcattt aattcacgca atttaagagg
    17761 atcaagctga agcttttgag ctatctgttc cataatatat tctataatgt aaatagcttg
    17821 gggagcacca aacccacgaa aagcagtatt aggtggaaga ttggtacgac aggcacgtcc
    17881 tctgatacgg acattaggta taaaataagc attttctgca tgaaacatag ctctttccag
    17941 aatggctata gaaaggtcag tgtaagctcc tccattgctt agtagttcta tatccagagc
    18001 ctcaatcttt ccttgagcat caaatcctac tttccagcgt ccctgaaaag gatgtcgctt
    18061 tcctgtagaa cgcatatctt cttctctttc taagagtact tgaacaggct tatgagttaa
    18121 aaaacagcct aaagctgcca ttgtagccca gatagttccg cctcgttctt ttccaccgaa
    18181 agctccacct aaacgaggga cttccacaat tatatgattg gcaggaaggt ttaacaatcg
    18241 ggagacgact tcttgaactt ccatagtgct ttgagttgca gtatgaagaa aaatctgacc
    18301 atcttctccc ggaatagcgc gacaacgctg agtttccagg tagaaatgtt cttgagttgg
    18361 tgtttcggtg attccttgaa gaataaattc tgattcagtt aaagcttttt gtacatcacc
    18421 agattctatc tttcgttccg ggacatacca ttcacctttt tcatcagctt cctcaatttc
    18481 aaagataggt ggtaattctt catattttag atggataagt ttaactgcag cttctgcaat
    18541 tttttcgtct tgagctaaaa ccattgccag aggctgacca atatacataa tctctttttc
    18601 agggaataaa ggttcatcct gaattaccag tcctatctga tttattccag gaatatctgc
    18661 tgccgtaata actttttcta ccccagggaa ggcacttgct tcgctaatat ccagttcagt
    18721 aatatttgca tgagctacag gtgaaaagag gaatttagca tagagcattt ctggtaaagg
    18781 aatttcatca gcgataaaat gtgatgagcc agttaaatgc aatttgccac tgatatgagg
    18841 ataggattta ttcattgaaa cctccttcaa ggaattcttg aagatagttt agaacctgat
    18901 tcctgattac cagctgacga tattcttttg aaccccgcac atctgatata ggattgaatt
    18961 catcagctat aagttctgca aactccaaag ggtttatttc ctctttactt tttccaatga
    19021 ttaattctgg aaatttagat gaaagttttg ggataggtgc cacacctcca aaagcaagtt
    19081 gggcttcttg aattattcct gcctctgata ctatctttat ggcactaact acagatgaaa
    19141 tatctactgc tttgcgtttt gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19261 ngatactatc tttatggcac taactacaga tgaaatatct actgctttgc gttttgtggc
    19321 tttagtggtt ctaataaatg catcttttgc cgggactgga atgattatct ctttgatgat
    19381 ttcttttggt tgtagttttg tttttctata atccagaaag aaatcttgta gaggaatttc
    19441 tctgatacca caggaagata atagggttaa agatgcatcc ataactaata aaagaggaag
    19501 agtatcacca ataggagaag catttgcaat atttccagcc agagtggcaa agttacggat
    19561 ttgcctggaa gctatcttgt taaccagttc gtatagtgaa ggtaaatcag atttaataat
    19621 tcctgaatgc tgtatatcgg aataggtaac tccagcacca attattattc cttgtttagt
    19681 ataatttatc tgtttcagct ctggtagttc gcttaaatca atcaagacag gaatctcttc
    19741 tttctggata ttcattctca ccatcacatc agtaccacct gttataaaga cagcttcggg
    19801 ttcatttttt aaaatatctg ttaattctga tagacttctt gggataagat aagaagtgca
    19861 agggaagagt agctttccac ttctttgcac ggtttctgat ttgtctttaa agcagaataa
    19921 tcttggttca attgctctac accaatctgg gataatatca gaagtggtaa aattttcaga
    19981 aagctctctt gttgctctaa gaatgctttg atagcttccg caacgacaga gatttccctc
    20041 taaggaggca aggatttcct cttcgtctgg atgggaagaa ttagctaaaa gggcaaatag
    20101 actcataaca aaaccaggag aacagtatcc acattgcaag gaatgctgtt tatacataac
    20161 ttcttgtata ggatgaagat gttcaggagt gcctaatcct tctatagtta ttaaatgttt
    20221 gccgtgtagt tttgcagcat tatagagaca ggaattgata gcttcataga ctatatttcc
    20281 ctcttttaca taggcaataa caactgtgca ggctccacaa tctccttcat tacagctaca
    20341 ttttgttcca tataagtgct gattagtatg taaaaactga agagtggttt gcgcaggagg
    20401 aatttctgca gtgatgggtt ttccattgag ccagaattgt attttattaa agttataggt
    20461 tctttgtaat aactttataa gattttcaga gttatgaact tccatatatt tcccttcctt
    20521 tttaaccttt aaatcttatt gttggataag ctgaaatttg tccagatttt ttattggaaa
    20581 taccttataa taaggagatt gtaatcttta taaaatgaaa gaaagtttta gaaaagcgtg
    20641 gttattattc catcttctgg gaaagccatt cagatattgt gctataaata gattcctgaa
    20701 attcctttat attttcctta cattcaagta gtatggtatc acaaccaata gtttcttttt
    20761 gagtatcttc aggcgactca taatgaccct ttttactgtt tttaggattt ccaggataga
    20821 ccagagcatt tttgtgagta ttaaatatat tagcataagc aaatatctgc attaaatctg
    20881 aatcggaagg tatagcatta atattttctg gaactttcca tttagtgtcc agaatataac
    20941 aatcagtctg tttatttgcc tcattagttt gttcttggat gataatatca ggcttaatct
    21001 ctcttttatt atattcggaa ctccagaata gttttttgcg ttgagcgtaa acattgtatt
    21061 ttgattgatt ccggataaat tgcttccgaa gtgttataaa gataaatttc tcccatagtt
    21121 tattcatatc aaacataagg gcaagaatat gatttgtgcc agaacttaaa tcgggatgaa
    21181 agtttagaag aagcagtttt gctatttccg ttgccg
//
LOCUS       DAORZF010000029        20877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_2158_length_20877_cov_333.620786, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000029 DAORZF010000000
VERSION     DAORZF010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 20877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20877
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2158_length_20877_cov_333.620786"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="PK015_04705"
     CDS             complement(<1..505)
                     /locus_tag="PK015_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33799.1"
                     /translation="MVKGKFKPVLLLVILFPLLGAFLGYLLTTSTQNNPSQVVSSTTE
                     EPVMVKGPYPVAEPLNKELTLIELTFYLAILCGIVALVYHYLSSSIAWIAIVLLPLIF
                     CILYQQRTGMLLTLFYLPTLALSVLIALMIKFLFFNKKVLHFRLILCTLLGAGIITSY
                     LYSLYLLT"
     gene            671..1621
                     /gene="ftsY"
                     /locus_tag="PK015_04710"
     CDS             671..1621
                     /gene="ftsY"
                     /locus_tag="PK015_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOL33800.1"
                     /translation="MSLINNLKKKLAGTKSSFLGKIAEAVKLRGKVDEELMEQIEEIL
                     LRCDTGVEMTDLIMETLREKIRMNKITDPEEVQRVLQAIMEEILLKDYDEAINPFENI
                     DTKPYIIVFVGVNGVGKTTSIGKLAHKFIQQGKKVMIIAADTFRAAAIEQLAIWSERA
                     GATLIKSQPNADPAAVVYDGIASALAKDYDVVLIDTAGRQHTRENLMKELSKIDRTIK
                     KLIPEAPHQVLLVVDATTGQNAISQATNFSKAIPLTGIVLAKYDGTAKGGIIFNLKHN
                     LNLPVLFIGVGETLEDLEPFDIRTFIEAFFTTTEENEQSA"
     gene            1650..4877
                     /locus_tag="PK015_04715"
     CDS             1650..4877
                     /locus_tag="PK015_04715"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HOL33801.1"
                     /translation="MSVIEQDFILQVYTRLRDIIFSSENLKNKFLLLRQLLESVYKKL
                     SEDSNMSFSGLFARMKYIQEQLEIPSEIDYQLNQLRILSNKAAHEDDYQPTSKEWLSS
                     VYAINKFLRWLNPEIEDSDIEDYLSKHQAQAFPPLSPQKRKDLLCIVKSWEVLKNNNQ
                     DAGLKIVGTLEEGIECTIYLNDMEDAGKKWSTLAKVLWQYCTLKCFRLTPIQGRAQNY
                     QSNPTTLIVLEPDFLIDTSYLAECFTNNEMHPEDYILNRMISEPSSEKQIQGIIVNNI
                     LDELIRTPDMPFKELFKKSLIKQPISLVALGNNTVLNIYQNILRIHFPALKEFAASLR
                     NVPVQLELSFFNPDYGLQGRVDILYEKDGKRHIVELKSGRAHIFDVWKNNKMQVIAYN
                     MMLKKSGRNPLGYASIFYSAAGENALRHISSDMALEQELIMCRNRIVGLIHNLAIDPI
                     PFFNWLKEQKVSSDINFREAKLSKIIETLQNLEDYEYEWFLEQVRLLCREIWFEKTGG
                     LNQNSIFGYNALWQESVNAKKQHYRILTSLKLEKIQFNELWFSIPDEDIITDFRIGDL
                     VVLYREKMRVEQQEIIRGKIKYIDRERVKVQLNGGCREIEDSKSDLWAMEHDIIESAL
                     YAPLSSIFDFLQDKSGKRSLFLGIREPNAGIFNSSKQKSIDDVVDRMFAAKDYFVVQG
                     PPGTGKTSGLLTQYIEKVFRETEHHILILSFTNRAVDEICLNLKKHSIPFIRTGQSET
                     IKQELLEHLIAEQDFDEIEKIIKSNRIWVATVQSCNAWLNDMLNIVKIDELVIDEASQ
                     IIENNILGIITKVGKTILIGDQNQLPPITLQTKETFKFQNQQLKSLCYDYYNKSMMER
                     LFQVCETRGWNHSITMLNEHYRMHNQIASLVQEYYADALIPKLPLQLQDLVPQGNILV
                     DSRLVWIEFPVSEHLYYDSLQVQYIIQILKVFKQYGIVQNYDKDVGIVTPFRAMIQAI
                     YKELPTDLRAITIDTVERFQGSERKNIIISLPLHHANDLSLTEALSSDGKVDRKLNVA
                     ITRAQERLIILGCSKLCQKSRHYNNLLHKIQSSGTMLNYQELFKL"
     gene            4888..5187
                     /locus_tag="PK015_04720"
     CDS             4888..5187
                     /locus_tag="PK015_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HOL33802.1"
                     /translation="MNKPQPPNQINIKIDENVGEGTYANFFVITNSASEFIMDFGRIL
                     PGLPDARIYSRVICTPQHAKQFLNLLQKNIESFESQYGEIKVQGVPENKPIGFKA"
     gene            complement(5357..5587)
                     /locus_tag="PK015_04725"
     CDS             complement(5357..5587)
                     /locus_tag="PK015_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOL33803.1"
                     /translation="MIEKEKIEEILNRVRPSIQADGGDVELVNIREDNVIELRLKGAC
                     NGCPMATLTLKAGIERIIKEEIPEVAEVIAVA"
     gene            complement(5672..6142)
                     /locus_tag="PK015_04730"
     CDS             complement(5672..6142)
                     /locus_tag="PK015_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33804.1"
                     /translation="MKKTFLLMTLLMLTSLILAVPVATHQKQLDMGAGLFNTHFPIYV
                     GLDYGLNENSTVGGGLSTAFYDHGNKFDVYGNWNYHFVNLLSMPHNTDLYAGLSLCLD
                     AWLGHEGHEDDHKSMLILTGQIGGRYYFNNNLGIHLEIGGCPTSNATFGISYKF"
     gene            complement(6266..9373)
                     /locus_tag="PK015_04735"
     CDS             complement(6266..9373)
                     /locus_tag="PK015_04735"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOL33805.1"
                     /translation="MFLSDLSVDRPVLVSMALFVFIVFGVLAYFSMPLNLMPDVKIPY
                     VVVQSVYPGAGPSEVETQVTKKIEDAIATVSLIDYIQSYSLENVSIVMVAFKMQKNVD
                     IANQEIKDKIDAIIRDLPSDLQKPIVQKMDINAIPFMDIILSGNLDGKALYELADTKL
                     KDRFGQIEGIAQVDISGGNKRQIQVQLNDQAVFQNKISLAQLMQILAAQNMDMPAGNF
                     QRGTQEYSVRLKGKFESLDEIANANIPTPTGIKKLRDLANVIDGAEDVRTRAIYFDVP
                     KKVLDQNIVHISLTNASDANVVTISDEVRKNLPEIEEDLPAGVSLNIVRDTSDFIKST
                     VSDTFTNIWLGILFTGLILLIFLHDLRSTLIVALSMPISIISTFVFMQIAGFSFNMMT
                     LIGISTSVGILVSNSVVVIENIFRHRDEGMPVRDAAKRGTAEIAIAVLASTLTNVVVF
                     LPVATMGSLVGQFFREFAYTVTIATIFSLVTSFTVTPMLASRILTGKEKKNKFGIAFD
                     NLFDKFEASYKKFLGTVLSSKKHSRNIIIISVILVVGSLALMPVIGLEFIPEIDQGEM
                     SITVELPEGYNLDQTADVLEIVHQRLANHKEIEHIITNLGSSGFVNTGTNLASARIKL
                     VDRADRKKSAKQLAEIFSIELADIPNAKIVVSAASSAMGQGMGMEFYLQGQDNARLEQ
                     MKNQITELIKDTPGLMNLDTSSRGGRPELTIIPKRDQMSAVNATVYDLAISLRAAVEG
                     MVSTEYHEGDNQYDIKISLADDAVDTPDKIRNLTILINGQPFLLSQLADIEVKPGASN
                     IIHRDRAKTIVFTGEIAEDATMGDVMNAVQEKLQGFNFPSGYKIVWGGGAKMLNETVV
                     AMLQAFLLAVLLTYMMLAAILESFLQPLFILGTVPLALIGVILSLLITHQTFNIVSMM
                     SVVMLVGVVVNNAILLLDYANMRRKQGAAPHDALMEAGEAKLKPIIMSTLSIVIGMLP
                     MAIGIGAAGRELRKPMGTVTIGGLLVATFMTLVIIPAFYYLVIQKENNRKNGKKNQVP
                     QIENQ"
     gene            complement(9384..10412)
                     /locus_tag="PK015_04740"
     CDS             complement(9384..10412)
                     /locus_tag="PK015_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOL33806.1"
                     /translation="MKKQYFLIIILSIALLLTGCGRKQSQFKTLQQIQKEQGIPVRVK
                     TVELETFVQELSYNASLGGKEESNGQSLVSEVVSSVNAKIGDRVSAGQIIVTFPRNTP
                     TAQYEQALTAYNAAKTAYERMRNLYANGAISRQELDNVETQYKVAQANLDASDKMINV
                     RAPISGIITNIAVSPGERSYPGQNLFTVSSTNGYKAKLMVADRDAMKVKIGTPATATW
                     ENITLNGKVSKISLALDPYQKAIPVEVTFPSNGQRISFGSTAQIKLQILTKPNCIVVN
                     REYVVNENDKQFVWINQNNHAIKREITTGLDNQLQFEVVSGLEPGEQLITEGLNLLTE
                     NALIRVIE"
     gene            complement(10429..11808)
                     /locus_tag="PK015_04745"
     CDS             complement(10429..11808)
                     /locus_tag="PK015_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOL33807.1"
                     /translation="MKRISIIIAFCLIVSMFWSISLDESISLAKQNNKDLLSSKEDLQ
                     KAEQTYYQVRANIYPQLNVVGAYSLNKTYLPDSAIVPPIDLSLGLDPDTASSNDYYLA
                     NVMSGALNSLVPSSPMEEGSMALSLQMQQVLFTGGKLNYGIKTADIYRNIQQLNYQLK
                     EQDIILKTTQMFYSCLLADKLVNVQEAALETARHHLARVEAFANEGLVSDFDVMRARL
                     EVAKLEPQLMQAQNSRDLALSAFRTQIGVNDPTIVPEGNLVLPPKLDITLDEALTQGL
                     ENRIELTMAKKGTEVADLQLKTEKAGYFPNLALQASAALYTKADEYAIEGDDFGTNYS
                     IGLGISFPLFNGFATKAKVNSAKSSYRQSQYQEQKYEDLIRLEITQNYQKLRHAEKNY
                     LVQEENMKMAERSLQLAQLRYENQVGIQLEVFDAQTTLSAIQLQYFQSIYEVISATRE
                     LQKSLGMNF"
     gene            complement(11805..12452)
                     /locus_tag="PK015_04750"
     CDS             complement(11805..12452)
                     /locus_tag="PK015_04750"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOL33808.1"
                     /translation="MNTGSDKKELILDSAIELFNRWGYAKTSVDEIARAAHIAKSTIY
                     YYFPSKENLFIAAIEEKAEEFFNKLTTAIEAAEKFEDKLSCFLSLPITCIFQNMPLLA
                     NAMQQIPPDYFASLLIKRNEYRQRMNNLLAEIMNYGKKQGIINDKINSEHFSEMINDW
                     FIMGDYWNEGDNKNRILKRLERDHVLMVQLLLYGILKRDTAKPRTRKKVNKRKIQ"
     gene            complement(12922..15729)
                     /locus_tag="PK015_04755"
     CDS             complement(12922..15729)
                     /locus_tag="PK015_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOL33809.1"
                     /translation="MAKSKSDVKDIKERLRQSSATAAKKPVKEYKVPEKPPAFDETLL
                     RPQALVPSKTNTIVAWIVFAFALIIYMLTQARSLSFWDSGEYATCISILGVPHPPGNP
                     FYILFGRALVSLFGNILPHAVIAAFISGLASAFAVMFTYLVTVQLTSMFKIKPWQSMF
                     AGVIAALYTAFSYTFWMNAVEAEVYAGLVFFINLIIWLTLKWVQNSRDFNKQNYLLLI
                     VYLFFLGFCVHQTALQIAPAILFIVTYPIFLQATTKKTFWYKFFGYGAALIITYLIFG
                     EIGKAFNIDSFDQLGFALCIIVLMYIDLHKVLDKKIWLLAIALIILGVSSYIYLPIRA
                     ADRPFINLADPSTPKRFDDYIQRRQYKPEQVTSMFARRGNFFEHQLGYHFLRYFGWQW
                     FKSDSVNRVTGIPAQVVNVFGGLFVAFLGLFGAVFHCKKNKHSFFYFLAIFFCTSLLM
                     VFVMNLSSAEVRDRDYFFVAAYNMWAIWLGIGAMGLVSLFNNDKDKVLRVVMAAIMLF
                     LPIFNMAAQYYEHDRSRELIALYYGVNFLNSVEENAIIFTNGDNDTYPLWYAQAVKDP
                     YAEPYVHQAREVYPTDESQEAIKRAMEYKNQQCKGIRKDVTVANLSLLNTSWYIRQLR
                     DLEGVIINWSEDEINSLDDRYGSFQKLLWKDSVTFDAGDPEGKMKFTINYRENFEKHE
                     TTGEFYPRRGSDFAVIQIIKDNFGKRPIYFAVTCESRVGFDDYLRNEGMVSRVVATYD
                     PVNEQIDIDRLLTNIDKVYKYDSIFDPKVYKDDNMKRLVMNYGSGFSRAAVYFAKNHQ
                     FEKAEEYAKKARAFIDSDIRLTEFYVTYYIEKGELDKLDAFIKNNIWGDRDEISIYRA
                     YVLRYVMENRQDLVPRYLAKIMARHPDEPELGAIALAYGYQYKQMSQIDALFDSLKDI
                     LHYTPEDIYRSIQQEMGNQSY"
     gene            complement(15745..16740)
                     /locus_tag="PK015_04760"
     CDS             complement(15745..16740)
                     /locus_tag="PK015_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOL33810.1"
                     /translation="MKKIFSNSIVYLFLIFFGIIMILPFIWMISTSLMSQAEFNKNES
                     IFIPKEEYYVWDENGKEHRIILVQQKEENSIIHVLDENLQISKDYPEYMSVPSSQVRL
                     IKKVPSFHWDNYITAFNKVPFARYFANTIFVSFLTLIGVLITSFLAAYAFARMEFKGR
                     DIIFYLFLSMMMVPEPIYIISSYMLLDKFNWLDTYQALIVPWCVNIFTIFLFRQHFKS
                     LPQELFDAAAIDGCSIFGMLWRIVLPLSKSVIATASVFSLIGSWNSFMWPLVMTDRPE
                     LRVLQVGLSYFSQEASTQTTLLMAASTFSILPILIIFFFAQKQIIASYAKAGLKD"
     gene            complement(16750..17682)
                     /locus_tag="PK015_04765"
     CDS             complement(16750..17682)
                     /locus_tag="PK015_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOL33811.1"
                     /translation="MKTKYKYEPYLYILPALVLLLLFRFIPILLSFVISFFDFGITSI
                     GNFIGFQNYNKLFVDPEFWQSMMNTVWLVIIAVPLSIIIPLIFAVLLNQIKWLKGFFR
                     TIYFMPYVTSLVAVSIVWKIIYAEEGGLANAFLQWLGFQPLKWLAEPKGIFTLLFNSM
                     GVSIPSWLGGPSLALFSIIIMTVWKSLGYNTIIYLSGLQNISKVYYEAAEIDGANKWK
                     QFFKITVPLISPTTFYVLLMTTITSFQTFAQVYMMTDKGGPLGTTKLIVYYIYEKGFD
                     DLQMGYASAAALILFVVILGLTLMQRRLESKVNY"
     gene            complement(17705..18901)
                     /locus_tag="PK015_04770"
     CDS             complement(17705..18901)
                     /locus_tag="PK015_04770"
                     /inference="COORDINATES: protein motif:HMM:NF005744.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOL33812.1"
                     /translation="MKLSQRAMEIQASPIRKLMPHAEAAKKRGLKVYHLNIGQPDIPT
                     PQVMIDAYHSFSDKVLAYGPSQGLDIYRQGLVRYYSRYGIDLNEDEIIVTTAGSEAIT
                     FAMMVVAEVGEEILVPEPFYTNYNGFATMAGIHLKPITTVGENGYQLPPAEVIESFIT
                     KKTKAILICNPGNPTGTVYPEEDVMMIGEIARKYNLFVISDEVYREFIYEGLKHTSIM
                     NVPDLEANAILVDSVSKRYSACGARIGCIASKNKEVMSALLKFAQARLCPPTVDQLAA
                     LACIDLGDEYFHPLISEYEARRDLAYNALLQIPDIVCVKPQGAFYILATLPVDNVEEF
                     VTWMLDEFSIDGATMMGAPGDGFYATPGLGLNEMRLAYVLNQEDLSKAMHILTEGIEA
                     YNKIKG"
     gene            complement(18975..20798)
                     /gene="uvrC"
                     /locus_tag="PK015_04775"
     CDS             complement(18975..20798)
                     /gene="uvrC"
                     /locus_tag="PK015_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOL33813.1"
                     /translation="MEKIPEKIAEKLPLLPDHPGIYLWKNAEGEIIYIGKAKSLKNRV
                     TSYFSSTNKDIKTEHLLKYIADLDYILVPTEQDAFILEANLIKRYKPKYNVLLKDDKR
                     YPFVKITLNEPFPRIIVTRDYVRDGSRYFGPFTDTKSLRMTLRNLEWIFPVRTCSRQI
                     PADRIKYQKACINYQLGKCPAPCIGAISQEEYMQIVKRQMRFLEGHHDEIIEEFKTEM
                     NNLSEELKFEEAAKLRDRIQAIEHIQKRQVVYSPDSRNTDVIGFYQEENIAICVVLRI
                     VNGMIINKEDYPLANVEENTPEEILAAFLKLYYSNRDELPDEILLPFEPSELEELNEW
                     LHGIISIPKRGEKSQLITMAKNNAFHLVEERKLAHLRKANRTIYPIQELKEKLSLPKL
                     PRKMVCLDISTIQGTDTVASAVFFENGKPKKKFYRHFIIKSLDTQNDYAALQETLTRY
                     LKECDENPEMLPDLFIIDGGKGQLNACLEVLKNSKYPDIPIISLAKRIEEVFTPANSQ
                     SIILPRSASALRLLIAIRDEAHRFAITFHRSRRSRRTLISELEEIPGIGEQTKFLLLK
                     ELGSVDAIKNASPEKLNSIKGIGPKTAENIYSYFHPKDSEK"
BASE COUNT         6928 a         3816 c         3691 g         6442 t
ORIGIN      
        1 aggtcaatag ataaagagaa taaagatagc tggttatgat acctgcacca agtaaagtac
       61 aaagtatcaa gcgaaaatgc aatacctttt tattaaagaa aaggaattta atcattaaag
      121 ctattaaaac agatagagct aaagtgggaa gataaaatag agttagtagc attcctgttc
      181 gttgctgata gagaatacaa aaaattaagg gtaacaaaac aatagcaatc caggcaatag
      241 atgaagataa ataatgataa accaatgcta caattccaca cagtatagcc agataaaagg
      301 taagctctat taaagttaac tctttattta gaggttcagc cacaggataa ggacctttca
      361 ccattaccgg ttcctcagta gttgaactaa ctacctgtga aggattattc tgcgttgaag
      421 tagttaaaag ataacctaaa aaggcaccta ataagggaaa taagataacc agcaaaagaa
      481 caggcttaaa cttccctttc accatatatt cctcagttaa tttttattga cttaatagta
      541 ttattacaat ttatggaaag caggatggat gtcaatcaga aactgaaact gactgtagat
      601 aaaccctttc cctgcactta ttcctttatt aaatcagggg aaaattagat ataacaattg
      661 gagaaactta atgagcctaa ttaataattt gaagaagaaa ctggcaggaa caaagagtag
      721 ttttttaggg aaaattgctg aagcagtaaa acttagaggc aaagttgatg aagaattgat
      781 ggaacagatt gaagagatac ttttaagatg tgatacaggc gttgaaatga ccgacttgat
      841 aatggaaact ttgcgtgaga agataagaat gaataagatt actgatccag aagaagtgca
      901 aagagtttta caggcaatta tggaagagat tctattaaaa gattatgatg aagcaataaa
      961 tccttttgag aatattgata ctaaacccta tattattgtc tttgttggtg ttaatggagt
     1021 tggtaaaact accagtatag gaaaattagc tcataaattt attcagcagg gtaaaaaagt
     1081 tatgataata gcagcagata cttttcgtgc tgcggctatt gaacaattag ctatctggtc
     1141 agaacgagca ggtgcaacac ttattaaatc acaacctaat gcagatcctg cagcagtagt
     1201 ttatgatggt atagcttcgg ctctggctaa agattatgat gtagttctaa tagatacagc
     1261 aggacgacaa catactcgtg aaaatctgat gaaagaactc agtaaaattg accgaaccat
     1321 aaaaaaactt attcctgaag ctcctcatca ggttctttta gtagtagatg ccacaactgg
     1381 tcaaaatgcc atttctcaag caacaaactt cagtaaagct attcctttaa ctggtatagt
     1441 cctggcaaaa tatgatggca ctgctaaagg aggaattatt ttcaatttaa agcataatct
     1501 taatcttcca gtgctattta ttggagtagg agaaactctt gaagacctgg aaccctttga
     1561 tatcagaact tttatagagg cattttttac taccacggaa gagaatgaac aatccgctta
     1621 actcattgtt acttgccgat taaagagtta tgtcagtaat agagcaagat tttatccttc
     1681 aagtttatac tcgtttacga gatattatat tcagtagcga aaacctaaag aataagtttc
     1741 tgctcttacg ccagcttctg gaatcagtgt ataagaaact cagtgaagat agtaatatga
     1801 gttttagcgg tttatttgct cgtatgaaat atattcagga gcaactggaa atcccatcag
     1861 agatagatta tcagctcaat caactcagaa ttttatccaa taaagctgca catgaagatg
     1921 attatcaacc tacttctaaa gaatggctaa gctcagttta tgcaataaat aagtttctcc
     1981 gatggttgaa tccagaaata gaagattccg atatagaaga ctatcttagc aaacatcaag
     2041 ctcaagcttt tcctccgctt tcacctcaaa agagaaaaga tttactctgt atagtgaaaa
     2101 gttgggaagt cctgaagaat aacaatcaag acgcaggttt gaagatagtt ggaacactgg
     2161 aagagggtat agaatgcaca atttatttga atgatatgga ggatgcaggt aaaaaatgga
     2221 gcaccctggc aaaagtactc tggcaatatt gcactcttaa atgttttcgt ttaaccccca
     2281 ttcaaggaag ggcacaaaat tatcaaagta atccgactac tcttattgtt ttagaaccag
     2341 attttttaat tgataccagt tatttagcag agtgttttac taacaatgag atgcatccag
     2401 aggactatat cctcaatcgg atgattagtg aaccttcttc agaaaaacag attcaaggaa
     2461 tcatcgttaa taacattctg gatgaattga taagaacacc agatatgcct tttaaagaat
     2521 tattcaaaaa aagcctgatt aaacaaccta tctctttagt cgctttaggt aataataccg
     2581 ttcttaacat ttatcaaaac atacttagaa tacactttcc tgctttaaaa gaatttgctg
     2641 catccctgag aaatgttcct gttcaactgg aactaagctt tttcaatcca gattatggtt
     2701 tacagggtcg ggtagatatt ctttatgaaa aagatggaaa aaggcatatt gtggaattga
     2761 aaagcggtag agctcatata tttgatgtct ggaagaataa caagatgcag gttatagcct
     2821 ataatatgat gctaaagaaa agtggcagaa atcctcttgg ttatgcaagt attttctatt
     2881 ctgcagctgg agaaaatgct cttcgtcata taagtagcga tatggctttg gaacaagaat
     2941 tgataatgtg taggaatcgt attgtaggtt tgatacataa tctggcaata gaccctattc
     3001 ctttctttaa ttggttaaaa gagcaaaaag tatcatctga tattaatttt agagaagcca
     3061 aactgagcaa gattatagaa actcttcaaa acctggaaga ttatgaatat gaatggtttc
     3121 tggagcaggt tcgtttactc tgtagagaaa tatggtttga aaaaaccggg ggtttaaatc
     3181 agaatagcat ttttggttat aatgcacttt ggcaggaaag tgttaatgca aagaagcaac
     3241 actatcggat actaacatca ttaaaactgg agaaaattca atttaatgaa ctctggtttt
     3301 ctattccaga tgaggatata ataactgatt tcagaattgg tgatttagtt gtgctatatc
     3361 gtgaaaaaat gcgggttgaa caacaggaaa ttatccgtgg aaagataaaa tatattgata
     3421 gagaaagggt gaaagtgcaa ttaaatggtg gctgtagaga aattgaagac tctaaatccg
     3481 atctttgggc tatggaacat gatattatag aaagtgctct ctatgcaccc ttaagttcca
     3541 tttttgactt tttgcaggat aaaagcggaa aacgatctct ctttctgggt attcgtgaac
     3601 ctaatgcagg aatctttaat tcttctaagc aaaagagcat agatgatgtt gtggatagaa
     3661 tgtttgcggc aaaggactat tttgtcgttc aaggacctcc tggaactggg aaaacttcgg
     3721 gattactcac tcaatatatt gaaaaagtct ttagggaaac agaacatcat attcttattc
     3781 taagtttcac aaatagagca gtagatgaga tttgtttaaa cttaaagaaa cattctattc
     3841 cttttattcg cacgggacag tcggaaacca ttaagcaaga attattagag catttgatag
     3901 cagagcaaga ttttgacgaa atagaaaaga ttatcaaaag caatcgcatc tgggttgcca
     3961 cagttcaaag ttgcaatgcc tggttgaatg atatgttaaa tatagttaag attgatgaat
     4021 tggtaattga tgaggcttct caaataatag aaaataatat actcgggata ataacgaaag
     4081 ttggtaagac catcctgata ggagaccaga atcaacttcc acccattact cttcagacta
     4141 aagaaacttt taaatttcag aatcagcagc taaaaagctt gtgctatgat tactataata
     4201 aatctatgat ggagcgctta tttcaagttt gcgaaaccag gggctggaat cattctatta
     4261 caatgcttaa tgagcactat agaatgcata atcagattgc cagtttagtg caggaatatt
     4321 atgctgatgc tcttatacca aaattaccgc tacaactgca agatctcgtt cctcaaggaa
     4381 atattctggt tgattctcgt ttagtctgga tagaatttcc agtttcagaa catctctatt
     4441 acgattctct gcaagtgcag tatattatcc agattttaaa ggtattcaaa caatatggca
     4501 ttgtccagaa ttatgataaa gatgtgggta ttgtaactcc cttcagagca atgatacagg
     4561 ctatctataa agaactacca actgatttga gagctattac aatagatacc gtggaacgct
     4621 ttcagggttc ggaaagaaag aatattatta tcagcttacc tttgcatcat gctaacgatt
     4681 taagtcttac agaagctctt tccagtgatg gaaaagtaga ccgaaaactt aatgtagcta
     4741 taacccgtgc tcaggaacgt ttaattatcc tgggctgttc aaaattgtgt caaaaatcaa
     4801 gacattataa taatctcttg cataaaatcc agtcttctgg aacaatgctt aactatcaag
     4861 agctatttaa attataagga gaaaaaaatg aataaacctc aaccacctaa tcagattaac
     4921 attaaaattg atgaaaatgt tggtgaagga acttatgcca atttctttgt aataaccaat
     4981 tctgcatcag aatttattat ggactttggt cgtatacttc ctggactacc agatgcgcgt
     5041 atttatagta gagtaatatg tactcctcaa catgcaaaac aattcctgaa tcttctgcaa
     5101 aagaatattg aaagctttga aagtcaatat ggagaaatca aagttcaggg tgttccagaa
     5161 aacaaaccaa taggatttaa ggcataagtt agaaaactaa aattatcttt aaattccaat
     5221 caaaatttat tcttagaata gctggctcaa atagcagtac attttcctgt aagataagga
     5281 gtagataaaa aaaatagaag gttaataaat ttaaccttct actttttaat agtactgagt
     5341 taataactct tatctttcaa gctactgcta taacttcagc aacttccggg atttcttctt
     5401 taattatgcg ttcaatgcct gcttttaaag tcaatgtagc cataggacat ccattgcaag
     5461 cacctttaag acgcaattca atgacattat cttcacggat attgaccaat tctacatcac
     5521 caccatcagc ttgaattgat ggtctgacac gatttagtat ttcttcaatt ttttcttttt
     5581 ctatcattgt atattccttt tgtaggtatt tactaacatt ttaatgctaa ccaggtaaaa
     5641 gccaaattaa cttttagctt ctcatttact cctaaaactt gtaagaaatg ccaaatgttg
     5701 cattggaagt aggacatcct ccaatctcca aatgaattcc taaattgttg ttaaaataat
     5761 aacgaccccc aatttgacca gttaaaataa gcattgactt atgatcatct tcatgtcctt
     5821 catgaccaag ccaagcgtct aagcacagac taagtccagc atagaggtca gtattatgtg
     5881 gcatagaaag cagattaaca aaatggtaat tccagtttcc atagacatca aacttgttac
     5941 cgtgatcata gaatgctgtg gataagcctc caccaacggt ggaattttca ttcaacccat
     6001 agtccaatcc tacataaatg ggaaaatgag tattgaagag gcctgcaccc atatctaact
     6061 gtttttgatg agttgctacg ggaactgcca ggattaaaga tgttagcatt aagagagtca
     6121 taagtagaaa agtttttttc ataagagtct cctttactgg aagtttatta tttgaaataa
     6181 atttattgat attgaataat tgtcaaggaa aagcactcag gaagctgaaa ataatagaac
     6241 tatcagcttc ctgagtagat ttattttatt gattttctat ttgtggaacc tgattctttt
     6301 tcccattttt tctattgttt tctttctgaa taaccagata ataaaaagca gggataatca
     6361 ccaatgtcat aaaggttgct actaaaagtc caccaatggt gacagtaccc ataggttttc
     6421 taagttctct tcctgcagct ccgataccaa tagccatagg taacattccg ataacgatag
     6481 ataatgtgct cataatgata ggtttaagtt tggcttcacc tgcttccatt aaagcatcat
     6541 gaggtgcagc accctgtttt ctacgcatat tagcataatc taaaagcagg attgcattat
     6601 taactaccac tccaaccagc ataaccacac tcatcataga gactatattg aaggtttgat
     6661 gtgttatcag aagggaaaga ataacaccaa ttagagccaa tggaacagtt cccagaataa
     6721 atagtggttg taagaagctt tccagaatag cagcaagcat catataagtt agcaaaacag
     6781 ccaggagaaa agcctgcagc atagcgacca cagtttcatt cagcatcttt gctccaccac
     6841 cccagacaat tttatatcca gaggggaaat taaagccctg aagtttttcc tgaactgcat
     6901 tcatcacatc acccattgta gcatcttctg ctatttctcc tgtgaaaact atagtttttg
     6961 ctctatcccg atgaatgata ttactggcac ctggtttaac ttcaatatca gccaactgag
     7021 aaagaagaaa gggctgtcca ttaatgagta tcgtgagatt ccttatctta tccggagtat
     7081 ccactgcatc atctgcaaga gatatcttaa tatcatactg attatctcct tcatgatatt
     7141 ctgtggagac cataccttca actgcggcac gaagagatat ggcaaggtca tagacagtgg
     7201 cattaactgc agacatctgg tctcgtttag gaatgatagt taattcaggt ctaccacctc
     7261 tactactggt atctaaattc attagtccag gagtatcttt tatgagctca gttatctgat
     7321 tcttcatttg ttccaaacga gcattatctt gaccttgcag ataaaattcc atacccatac
     7381 cctggcccat agcagaactt gcagcgctta ctacaatttt tgcattgggt atatctgcaa
     7441 gttctatact aaatatttct gccagttgct tagcactttt cttacgatca gctctatcaa
     7501 ccagttttat tcttgcagaa gccaggtttg ttcctgtatt aacgaatcca gaagagccca
     7561 ggttagtgat tatatgttct atttctttat gattagctaa acgttgatgt actatttcta
     7621 atacatctgc agtctgatca agattgtatc cttcgggtaa ttcaaccgtt atagacattt
     7681 caccctggtc tatttctgga ataaattcca gtccaatcac aggcattaag gctaaacttc
     7741 ctacaactaa aattacggat atgatgatga tattccgact gtgttttttg gagcttagca
     7801 ccgtacccag aaacttctta tatgaagctt caaatttgtc aaagaggtta tcaaacgcta
     7861 taccgaattt attctttttc tctttaccag ttaaaatacg agatgccagc attggagtaa
     7921 ccgtgaagga agttaccaat gaaaatatag tagcaatggt aaccgtgtag gcaaattcac
     7981 ggaagaactg acctaccaga cttcccatag ttgctacagg caagaagact acgacattgg
     8041 ttaaggtact ggctaagacg gcaatggcaa tttcagcagt tcctcgttta gcagcatccc
     8101 gaacaggcat accctcatct ctatgtctaa aaatgttttc tataactacc actgagtttg
     8161 aaaccagaat acccactgaa gtggagatac cgattagagt catcatattg aagctgaaac
     8221 cagcaatctg cataaataca aaggtagata tgatggatat aggcatagag agtgccacaa
     8281 ttagggtgga acgtaagtca tgcaggaaaa ttaaaagtat cagaccagtg aaaagaattc
     8341 ccaaccagat attggtaaag gtatcgctaa cggtagattt aataaaatcg gaggtatctc
     8401 taacaatatt gaggctaaca ccagctggaa ggtcttcttc tatttcaggg agattttttc
     8461 ttacttcatc tgatatagtt accacattag catcggaagc attggtcaaa gaaatatgca
     8521 cgatattttg gtctaaaacc tttttaggaa catcaaaata gattgctctg gtgcgaacat
     8581 cttctgcgcc atctataaca ttagccaggt cgcgaagttt ttttattccc gtaggagtag
     8641 ggatattggc attagcaatt tcatccagac tttcaaattt accctttaaa cgaacggagt
     8701 attcttgagt gcctctttga aagtttcctg ctggcatatc catattttga gcagccagaa
     8761 tttgcatcag ttgtgccagg gagattttat tctgaaatac tgcttggtca tttaactgaa
     8821 cctgtatctg acgtttattt ccaccactga tgtcaacctg agcaatacct tctatctgtc
     8881 caaaacggtc ttttagctta gtatctgcca gttcatataa agcttttcca tcaagattcc
     8941 cagatagaat aatatccata aaggggatag catttatgtc cattttctgg acaataggtt
     9001 tctgtaagtc agaaggaagg tcacgaatta tagcatcaat tttatccttt atctcttgat
     9061 tggcaatatc cacatttttt tgcattttaa atgcaaccat cacaatgcta acattttcca
     9121 gcgaataaga ttggatataa tctattagac ttacggtagc tatagcatcc tcaatctttt
     9181 tagtcacctg tgtttcaact tcactgggtc cagcaccagg ataaactgat tggacgacta
     9241 catagggtat ttttacatct ggcattagat tcaaaggcat agagaaatag gctaacactc
     9301 caaatacaat gaatacaaat aatgccatag agaccaggac tggtctatca acagaaaggt
     9361 cggataggaa catctatccc tccttactca attacgcgaa tcaacgcgtt ttcagttaac
     9421 aggttaagac cttcagtaat taattgctca ccgggttcta aaccagaaac gacttcaaat
     9481 tgaagttgat tatccaagcc tgtagttatc tcacgtttaa tagcatgatt attctgatta
     9541 atccagacaa attgcttatc attttcgtta acaacatact cacgattaac aacgatgcaa
     9601 ttaggttttg tgagaatttg caattttatc tgggcagtag aaccgaagct tatgcgttgt
     9661 ccattgctgg ggaaagtaac ttctacagga atggcttttt gataagggtc taaagcaaga
     9721 gagattttac tgacctttcc gtttagcgta atgttttccc aggtggcagt ggcaggagtg
     9781 ccaattttca ctttcatcgc atctctatct gcaaccatca atttcgcttt atatccatta
     9841 gttgatgaaa cagtgaaaag attttgacca ggataacttc gttcacctgg actaactgca
     9901 atattggtga taataccact aataggagca cgaacattaa tcattttatc actggcatct
     9961 aaattggctt gagcaacttt atattgggtt tcaacattat ccagttcctg acgagatatt
    10021 gcaccattag catacagatt gcgcattctt tcatatgctg tttttgcagc attataagca
    10081 gtcaaagcct gttcatattg agcagtgggt gtattcctgg ggaaagttac tataatttgt
    10141 ccagcagaaa cacggtcacc aatcttagca ttaacagagc taactacttc tgagactaaa
    10201 gattgcccgt ttgattcttc ttttccaccg agtgatgcat tataactcaa ttcttgaacg
    10261 aaggtttcca attccacagt tttcactcta acaggaatac cttgttcttt ctgaatctgt
    10321 tgtaaggtct taaattgtga ttgctttctt ccacaaccgg tcaataaaag agcaatactt
    10381 agtattatga ttaagaaata ctgtttcttc attttgtttt actccatttt aaaaattcat
    10441 acctaaagat ttttgaagct ctctggtagc agagatgact tcatatattg attgaaaata
    10501 ttgtaattgt atagcagaaa gagtggtctg agcatcaaaa acttccagtt ggataccaac
    10561 ctgattttca taacgcaatt gcgcaagctg caaactacgt tctgccattt tcatattttc
    10621 ttcttggaca agatagtttt tttccgcatg tcttaatttc tgataatttt gggtgatttc
    10681 caaacggatt aaatcttcat atttctgttc ttgatattga gattgccgat agctggactt
    10741 ggcagaatta acttttgctt tagtggcaaa accgttaaag agagggaaag atatgcctaa
    10801 acctatgcta taattggttc caaaatcatc accttctata gcatattcat cagctttagt
    10861 ataaagagca gcgcttgcct gaagagcaag attgggaaaa tatccagctt tttcagtctt
    10921 caattgtaaa tctgcaactt cggttccctt ttttgccata gtcagttcaa tcctattttc
    10981 caaaccctga gttaatgcct catcaagagt aatatccagt tttggtggaa gaacaagatt
    11041 cccttctgga acaatggtgg ggtcattaac accaatctga gtacggaaag cggacaaggc
    11101 aagatctcgg gaattctgtg cttgcatcag ttgtggctca agtttagcaa cttccagacg
    11161 agctcgcata acatcaaaat cggagaccag tccttcatta gcaaaagctt ctacgcgagc
    11221 cagatgatgt cttgctgttt ccaaagctgc ttcctgtaca ttaactaatt tatcagctaa
    11281 aaggcaggaa taaaacattt gagtggtctt aagtattata tcctgttctt tcaattgata
    11341 atttagttgc tgaatgttac gataaatatc agcggtcttg ataccatagt ttaatttccc
    11401 acccgtaaaa agtacctgtt gcatctgtaa tgagagagcc attgaaccct cttccattgg
    11461 agaggaggga actaaggaat ttagagcacc agacattaca ttggctaaat aataatcatt
    11521 agaactggca gtgtcagggt ccagtcctaa gcttaaatct atgggtggaa caatggcaga
    11581 atcgggaaga taggtcttat ttaaactata agcacctaca acattgagtt gaggatagat
    11641 attggcacgc acttgatagt aagtctgttc agctttttgt aaatcttctt tggaagacaa
    11701 taaatcttta ttattttgtt ttgccagaga aatgctttca tccagactta ttgaccaaaa
    11761 cattgagact atcaagcaaa atgctataat aatactaatg cgtttcattg gattttcctt
    11821 ttattcactt tttttcttgt ccttggtttt gctgtatccc gtttaagaat gccatataaa
    11881 agtaattgaa ccattaaaac atggtctctt tccaaacgct tcaagattct gtttttatta
    11941 tctccttcat tccagtaatc tcccataata aaccagtcat taatcatctc gctaaaatgt
    12001 tcagaattga ttttatcatt tattatgcct tgttttttcc cataattcat aatttcagcc
    12061 agaagattat tcattctttg acgatattcg tttcttttta ttaataaact tgcaaaataa
    12121 tccgggggaa tttgttgcat agcatttgcc agaagaggca tattctgaaa gatgcaggtt
    12181 attggtaggc tgagaaaaca ggagagttta tcttcaaact tttctgctgc ttctatagca
    12241 gtggttaatt tattaaaaaa ctcttctgct ttttcttcaa tagctgcaat aaaaagattt
    12301 tctttactcg ggaaataata ataaatagta gattttgcta tatgtgcagc ccgagcaatt
    12361 tcatccactg aggtcttagc atagccccaa cgattaaata attcaatagc tgaatccagt
    12421 attagctctt ttttatctga tcctgtattc attatacata cctaatccct ttattattaa
    12481 tcaaatatga catagatgct gaatctaaat ttgaatttct gaccaaatca gttagaatgg
    12541 tcgaaaagtc aagaaatttc tattagtttt ttcaccattg ttatgctcta aagcctttta
    12601 ttatcaaggt ggaaggataa tttcaatcta tccagaatac aattttcttc ataaaaagct
    12661 ctaagactta taaattaaga tgaaaaataa ataagcaaaa attgattgtt aactgagagt
    12721 gaaataaaat attacaaaat atttatcccc tgtctcctcc ttgcctttcc cttggctgtt
    12781 taggtaagaa agtaacagga gagagccaag ggagacttag taaggagcta aaatctcaag
    12841 aattacttag taactccctc tttcccgcag ataatacatt tgcggggtta cattatttat
    12901 ccttaaaata aaatgtgtag cttaatagct ttggttaccc atttcttgtt gaatagaacg
    12961 atagatatct tcgggtgtat aatgaaggat atctttcaga gaatcaaaca gtgcatcaat
    13021 ctgagacatt tgtttatatt gataaccata agccagtgca atggcaccta actctggttc
    13081 atcaggatgt cttgccataa tttttgctaa gtatcttgga acgaggtcct gtctattttc
    13141 catcacatat ctaagcacat aagcgcggta aatgctaatt tcatctctat ctccccagat
    13201 attattttta ataaaagcgt ccagcttatc cagttcccct ttctcaatgt aataagtcac
    13261 atagaattca gttaagcgaa tgtcactatc aataaaggca cgagcctttt ttgcgtactc
    13321 ttctgccttc tcaaattgat gatttttagc aaaataaacg gcagcacgag aaaaaccaga
    13381 gccataattc atcactaacc gtttcatatt atcgtcttta tacacctttg gatcaaagat
    13441 ggagtcatat ttatatacct tatcaatatt ggtgagaaga cgatcaatat ctatctgttc
    13501 attaactggg tcataagtag caacgacacg agaaaccata ccttcattac gcagatagtc
    13561 atcaaatccc actcttgact cgcaggtaac agcaaagtag ataggtcttt taccaaagtt
    13621 atcctttata atttgtataa ctgcaaaatc tgaacctcta cgaggataaa actcaccggt
    13681 agtttcatgt ttttcaaagt tttcccgata attaatggta aacttcattt tcccttcagg
    13741 gtcaccagca tcaaaggtaa ccgaatcctt ccacaataat ttttgaaagc tgccataacg
    13801 gtcatccaga gaattaatct cgtcttcgct ccaattgata ataactcctt ccaggtctct
    13861 taattgacgg atataccaag aagtattgag taaagaaaga ttcgctacag taacatcttt
    13921 acggatacct ttacattgct gattcttata ttccatagct cttttgatag cttcttgaga
    13981 ttcatcggtg ggataaacct cgcgagcttg atgtacataa ggttcagcat aagggtcttt
    14041 aactgcttgt gcataccata gcggataggt atcattatct ccattagtga aaatgatggc
    14101 attctcttcc acagaattga ggaaatttac accataatac agagctatta actcacggga
    14161 acgatcatgt tcataatatt gtgctgccat attaaagatt ggtaaaaata gcattatcgc
    14221 agccattacc actcttaaaa ctttatcttt atcattgttg aataatgaga ccaaacccat
    14281 agcaccaatt cccagccata ttgcccacat attataagct gcgacaaaga aataatcccg
    14341 atctcgcact tcagcactgg aaagattcat cacaaaaacc atcagcaatg aggtgcagaa
    14401 aaagatagcc aggaaataga agaaactgtg tttgttcttt ttacagtgga acacagctcc
    14461 aaataacccc agaaaggcaa caaataaacc tccgaaaaca tttactactt gtgctggaat
    14521 acctgttact cggttaacag aatcggattt gaaccattgc cagccaaaat atctgagaaa
    14581 atgataacct aattgatgct caaagaaatt tcctcttcgg gcaaacatag atgttacctg
    14641 ttctggttta tactgtcttc tctgaatata atcatcaaat cttttaggag tgcttggatc
    14701 cgctaaatta ataaaaggac gatctgctgc tctaatagga aggtagatat aagaagaaac
    14761 tcccagaata ataagagcaa tagcaagcag ccaaatcttt ttgtccagca ccttatgtaa
    14821 atctatatac atcaggacaa taatgcatag agcaaaaccc agctggtcaa aagaatcaat
    14881 attaaaagcc tttcctattt ctccaaaaat cagataagtg ataatcaaag ctgcaccata
    14941 gccaaagaac ttataccaaa aggttttttt agttgtagct tgtaaaaata taggataggt
    15001 tacaatgaaa agaatggcag gtgcaatctg caaagctgtt tgatgcacac aaaaaccaag
    15061 gaagaataaa tatacaatca aaaggaggta gttctgttta ttgaaatctc gtgaattttg
    15121 cacccatttc aaggttagcc agattatgag attaataaag aagaccagac cagcataaac
    15181 ctcagcttct actgcattca tccagaaagt ataggaaaaa gcagtatata aagctgcaat
    15241 cacaccagca aacatagatt gccagggttt tatcttaaac atactggtca gttgaactgt
    15301 gaccagataa gtgaacatta ccgcaaaagc agaagcaaga ccagaaataa aagcggctat
    15361 tacagcatga gggagaatat taccaaagag cgaaactaac gctcttccaa aaaggatata
    15421 aaaaggattt ccaggtggat gtggaacacc cagaatactg atacaggtgg catattctcc
    15481 ggaatcccaa aaggataaag accttgcctg agttaacata tagataatta aagcaaaagc
    15541 aaagacaatc caggcaacaa tggtattagt tttagaaggc actaaagctt gaggtctgag
    15601 caaagtttca tcaaaagcgg gtggtttctc tggaacttta tattctttaa ctggcttttt
    15661 cgctgcagtt gcagaacttt gtctaagccg ttcttttata tcctttacat cagatttact
    15721 tttagccatt tatctatctc ccttttaatc ttttaagcca gctttggcat aactggcaat
    15781 gatttgtttt tgagcgaaga agaaaataat taagatagga agtatactaa aggtagaagc
    15841 agccatcaaa agtgttgttt gagtggatgc ttcctgactg aaataactta accccacctg
    15901 cagaactcgc aattcgggtc tatctgtcat aaccaggggc cacataaaac tattccagct
    15961 accgattaaa gaaaaaactg aagcggtggc tataacagat ttagaaagag gaagcacaat
    16021 tctccagagc attccaaaaa tgctacaacc atcaattgcg gcagcatcaa acagttcttg
    16081 cggtaaggat ttgaaatgtt gacggaaaag gaaaatggta aaaatattaa cgcaccaggg
    16141 aactatcaga gcttgataag tatccaacca gttaaattta tccagcaaca tatatgagga
    16201 aataatatat ataggttcgg gcaccatcat catacttaag aagagataga agataatatc
    16261 tctaccttta aattccattc gggcaaaagc ataagcagct aaaaaagagg ttattagcac
    16321 gcctatcaga gttaagaatg agacaaatat ggtattagca aaataacgag caaaaggcac
    16381 tttattgaaa gcagtaatat aattatccca atgaaaactg ggaaccttct tgattaatct
    16441 aacctgagaa gagggcacac tcatatattc agggtaatct ttagatatct gaagattctc
    16501 atctaagaca tgaataatac tgttttcctc tttttgttgg accaggatga tacgatgttc
    16561 ttttccattt tcatcccaga cataatactc ttccttagga ataaatatag attcattttt
    16621 attaaattct gcttgcgaca ttaaagaggt tgaaatcatc cagatgaagg gtagaatcat
    16681 aataattcca aagaatatca ggaaaagata gacaattgaa ttactgaaga tttttttcat
    16741 cttgcacctc taataattga ccttactttc cagacgtctt tgcattaaag ttaaaccaag
    16801 tatgaccaca aacaaaatta gtgctgcagc actggcatat cccatctgaa ggtcatcaaa
    16861 tcccttctca tagatataat agactattaa cttagtagtt cctaagggtc cacccttatc
    16921 tgtcatcata tagacttgag caaaagtctg aaaactcgta atagtcgtca tcaaaagaac
    16981 atagaaagtg gtaggtgaaa ttagtgggac ggttatttta aagaattgtt tccatttatt
    17041 agcaccatca atttccgctg cttcataata gaccttagag atgttttgca atccagaaag
    17101 atagatgatg gtattatatc ccagactttt ccatacggtc ataatgataa tggaaaaaag
    17161 agccagcgaa ggacctccta accaggaagg aatagagact cccatagaat tgaataataa
    17221 ggtgaaaatt ccttttggtt cggctaacca tttaagaggt tgaaaaccta accattgaag
    17281 aaaggcattg gctaaacctc cttcttctgc ataaataatc ttccagacaa tggaaacagc
    17341 taccagagag gtcacataag gcataaaata gatggtacga aagaaacctt ttagccattt
    17401 tatctgattt aaaaggacag caaaaattaa aggtatgata atacttaagg gtacggcaat
    17461 aattacaagc cataccgtat tcatcataga ttgccagaat tcagggtcaa caaagagttt
    17521 attgtaattc tggaatccaa taaaattgcc aatactggta attccaaaat caaagaaact
    17581 gatgacaaag gacaataaga taggaatgaa acgaaataga agaagtaata ctaaagctgg
    17641 aaggatatat aaataaggtt catacttata cttagtcttc atcagctcat cctcaaatgt
    17701 ttagctaacc ttttatttta ttataagctt caattccttc agtgagaata tgcatagcct
    17761 tgctcaaatc ttcctgatta agaacatagg caaggcgcat ttcatttaat cccaaacccg
    17821 gagtggcata aaaaccatca cctggagctc ccatcatagt tgctccatca atgctaaatt
    17881 catccaacat ccaggttaca aattcttcca cattgtccac aggtaaagta gctaagatat
    17941 agaaagctcc ctgtggcttt acacatacta tatcaggaat ttgcaagagt gcattatagg
    18001 ctaaatctcg tcttgcttca tattcagaaa tcaaaggatg aaagtattca tcccctaaat
    18061 ctatacaagc aagcgctgca agttggtcta ccgttggagg acacaatctg gcttgagcaa
    18121 attttaagag agcactcatt acttctttat tctttgaggc aatacaacca attctggcac
    18181 cacaggcact ataacgcttg gagacactat caactaagat ggcatttgct tccaggtcag
    18241 gaacattcat aatactggta tgctttaaac cttcataaat aaattcccta taaacttcat
    18301 ctgatattac gaataggtta tactttctgg caatttcacc aatcatcata acatcttctt
    18361 ctgggtaaac tgttccagtt ggatttccag gattacaaat taaaatggct ttagtctttt
    18421 tggtgatgaa tgattctatc acttctgcag gaggaagttg ataaccattt tctccaactg
    18481 tagtgatagg ttttagatgg attcctgcca tggtggcaaa accgttataa ttagtataaa
    18541 aaggttcagg aacaaggatt tcttcaccaa cttctgctac taccatcata gcaaaagtta
    18601 tagcttctga accggcagta gtgacgataa tttcatcctc attaaggtca attccatagc
    18661 gagaatagta tctaaccagt ccctgtcggt aaatatctaa gccttgagag ggaccataag
    18721 ccaggacttt atcagaaaaa ctgtgatagg catcaatcat aacctgcgga gtaggaatat
    18781 ctggttgtcc aatattcaaa tgataaactt tcaaaccccg cttcttagct gcttcagcat
    18841 gaggcatcaa tttacggata ggagacgctt gaatctccat agctcgttga gatagcttca
    18901 tttattcacc tcattaatgt ttttttcaag ttttatgcta ttgcaggatt tgacaaggaa
    18961 aattttgctt tctatcattt ctctgagtct ttaggatgaa aataggaata tatattttct
    19021 gctgttttag gaccaattcc ttttatagaa ttcagttttt ctgggctggc attttttatt
    19081 gcatcaacag aaccaagctc ctttaaaagc aaaaatttag tctgttcacc aattcctggt
    19141 atctcttcca actcactgat taaagttcta cgagacctgc gtgaacgatg aaaggtgatg
    19201 gcaaaacgat gagcttcatc tcggatggca attaataatc tcaaagccga agcagacctg
    19261 ggcaaaatta ttgattgtga attagctggt gtaaagactt cctctatcct tttagccaga
    19321 gagattatag gtatatctgg atatttagaa ttctttaaaa cctccagaca ggcattaagt
    19381 tgtccttttc ctccatctat gatgaacaaa tcaggtagca tttcaggatt ttcatcacat
    19441 tcttttaaat aacgggttag agtttcctgg agagctgcat aatcattctg tgtgtccaaa
    19501 cttttgatta tgaaatgacg ataaaacttc tttttgggtt tcccattttc aaagaagaca
    19561 gctgaagcaa ctgtatcagt tccctgaata gtagaaatat ccaggcagac catctttctg
    19621 ggtaatttag gcaggctaag tttttctttc aattcttgga taggataaat ggttctatta
    19681 gcctttcgca gatgtgctaa tttgcgttcc tccaccagat gaaaagcatt attttttgcc
    19741 atagttatca gttgactctt ttcaccacgt ttagggattg aaataattcc gtgtaaccat
    19801 tcattcagtt cttccagttc agaaggttca aaaggtaaaa gaatctcatc aggcaattca
    19861 tctcggttac tataatataa tttcaggaaa gcagctaaaa tttcttcagg agtgttttct
    19921 tccacattag ccagaggata gtcttcttta ttaataatca tcccattaac aatcctcaga
    19981 actacgcaga tagcaatatt ttcttcctga taaaagccta taacatcggt attacgagaa
    20041 tctggagaat aaaccacctg tcttttctgg atatgttcta tagcctgaat gcggtctctt
    20101 aattttgccg cttcttcaaa tttcagctct tcggaaagat tgttcatctc tgttttgaat
    20161 tcctctataa tctcatcatg atgaccttct aaaaatcgca tctgtctctt gacaatctgc
    20221 atatattctt cctgagaaat agcaccaatg caaggagcag gacatttccc taactgataa
    20281 ttgatacagg ctttttgata tttaattcta tctgcaggaa tttgacgaga acaggttctt
    20341 actggaaaaa tccattccag gtttcttaag gtcatacgta aggatttagt atcggtgaaa
    20401 ggaccaaaat agcgtgaacc atctctaaca taatcacgag tgacaataat tctggggaaa
    20461 ggttcattta aagttatctt tacaaaggga taacgtttat catccttaag taatacatta
    20521 tattttggtt tataacgctt tattaaatta gcttctaaga taaaggcatc ctgttccgta
    20581 ggtacaagaa tataatccag atcagcgata tatttcaata aatgttcagt cttgatatct
    20641 ttattagtag aactaaaata ggaagttacc cgattcttta aagatttagc tttcccaata
    20701 tagattattt caccctcggc atttttccag aggtaaattc caggatgatc tggtaataaa
    20761 ggaagttttt ctgctatttt ttctggaatt ttttccataa attagttcct gcttccttta
    20821 gataatccag tttaaataat agtccaagaa catagaataa tccgatactt gagacag
//
LOCUS       DAORZF010000030        19124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_2396_length_19124_cov_355.087472, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000030 DAORZF010000000
VERSION     DAORZF010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 19124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19124
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2396_length_19124_cov_355.087472"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="PK015_04780"
     CDS             complement(<1..833)
                     /locus_tag="PK015_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33814.1"
                     /translation="MKKALLLLLIMTILVSGYAIDDRIPRPLVHLKNIQPYNVDYQQD
                     LVILRNQNFIWIYSIFNPWSPRLEAVFTSSALIEDLNLLGDNHLYVCSEEPSNTVTSV
                     DTLSYPGKIFFTTTVIGNKIAREGSILYVADRFRGIDIIDIGRGGMRELKSTFSEKWG
                     IRDLLAEYPYIYALNDFGLVTIDISDLQFPISLGVNYEIADATVLAKYGNIIWIGAGK
                     KLLAINVADMKHPRLINLMYLANEIQDLEIYNDRLFVALGQGGVKIYGIKNPLKIEDL
                     NT"
     gene            complement(916..1896)
                     /locus_tag="PK015_04785"
     CDS             complement(916..1896)
                     /locus_tag="PK015_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOL33815.1"
                     /translation="MNSEEMIQKIQELKQVKEDLLTQIRKTIVGQDEVIDKVLIGLFA
                     NGHMLIEGVPGLAKTLLISTIAKALNLTFGRIQFTPDLMPSDITGSDILVTSRDSERR
                     EFQYIKGPIFANLILADEINRTPPKTQAALLQAMQEYQVSSGNTTRPLDLPFIVMATQ
                     NPIEQEGTYPLPEAQLDRFMLYINIDYPSYEEELTIVQNTTGVPQEEPLPLLSAEKIL
                     QYQAAIRTMPVSQHILEYAVKLARSTRPGTPESRPEITKWINWGAGPRASQYLVLAAK
                     ARAALNGRLTPAEEDIRAVALPVLTHRVLVSFAAEAEGIKPSDIIKQLLS"
     gene            complement(1886..2893)
                     /locus_tag="PK015_04790"
     CDS             complement(1886..2893)
                     /locus_tag="PK015_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOL33816.1"
                     /translation="MSRKRILYLIIGLIIGIVLIILWLHYIDLNELVARMKRIDTRLV
                     FWATISYLAAYFIRSVRWNILLSKQTSISIWKTYLYSMGGNWVNYLIPIRAGELVKAW
                     FVKHNHGNSITSVLPSIFIDKTFDTLGIFFVLLMIPLLKVKFSQGLIILLILLGLVFA
                     VSLAVLLLSVWQKDKVVRFLQVLFSWLPKKLKTKVNNYISIFVQGLNIFEHHWTRLLN
                     SFLLTALGVFLDGLYFYLLFLAFGIDYPFLKVLFGYTLINLSYAIPQPPAQLGSNEWM
                     MIIIFSVGFALTREDASAIMAFAHMLTAILIGVIGLVSIALSGRQVLKAVLSGEKIYE
                     Q"
     gene            complement(2952..3794)
                     /locus_tag="PK015_04795"
     CDS             complement(2952..3794)
                     /locus_tag="PK015_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOL33817.1"
                     /translation="MAIPIINDWEKFFSNPYEGLGSSYERIVLNDLLFQTVNNYNVHN
                     VLESPSFGFTGISGINLLNLADLGLNITLEDNDLHRLDLITDLWKKLGRNFNPVYNPD
                     FHELNYPDKSFDMSFSFSALWFVPDLSHFLKELSRVTSQVIFISIPNRNGLGFKMQIK
                     DYTPEKYPQIKLSFIDPPSIIHILKKLDWYLVDSGYFDCPPWPDIGMTKEELLKKWFP
                     QLPISKIFKEQPDKESSSILDYYSGKDPDFAPRMRRYQWLEKTAPDAFKKVWAHHYYM
                     IFAP"
     gene            complement(3794..4768)
                     /locus_tag="PK015_04800"
     CDS             complement(3794..4768)
                     /locus_tag="PK015_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOL33818.1"
                     /translation="MQRLLITGVTGLIGRSVLNSILKEQLDYEITALIRPNTALERFS
                     SFRSSVELAQIDLADTERLYDYLKKNSFDVVMHIGALRGGRKFPRDIYIKANYYSTEQ
                     FVEHCQKHGSRFIYCSSVGVFGVIPKELPATNETERNPDSLYHWTKIESERIINQAVL
                     KGLNAAILRPSITYGPGDYGFPYQMVKLVQKKLFPIINKRIWIHLCNVNTITRAFLWL
                     LKNEYTPGLALNVADREPVQLRNLVDFISREIHGKNYNTILKLDRNLFALGESIARKL
                     KNELWTSRFQLISHSWFYYVDNTYELMNLPPTFTIPDIRIIIDDILKK"
     gene            complement(4769..5557)
                     /locus_tag="PK015_04805"
     CDS             complement(4769..5557)
                     /locus_tag="PK015_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HOL33819.1"
                     /translation="MIDTHCHLVPNIDDGSSSFETSLKLLRQMVEDGITHAFLTSHYL
                     PGLYQYDRALYDDRFEELQSQVQKEGINITLYPGFEVLLNPTSLTDIERFNLTLGNSH
                     YVLIESDLNGLPKDFYENIYPLLRNGYRPVLAHAERYVSIMHKPHNAKELMEKDIYLQ
                     INAGSLLGKYGEKVRQTAWVLLDNGWAHLLGSDSHANEPNNLFPQAIKLIKERIDEHT
                     VQLLTLDFPQMIINDEQIPLFYVYIQSSHHHHHHHKSFWERIFG"
     gene            complement(5561..6415)
                     /locus_tag="PK015_04810"
     CDS             complement(5561..6415)
                     /locus_tag="PK015_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOL33820.1"
                     /translation="MIETSLKEYLGTQSGPNKQLKQAMHYAVFSGGKRWRPLLLLSIY
                     EMLMGMRKNTGLPQALKAATAVELVHNASLVHDDLPIIMNRTERRSQPALHTKFGNAT
                     AILTGDALYTLAFEVLGQISPPSKALSCIRILSNSASSFGLIGGQAVALESKHKVMKM
                     NTLHYIDLKKVGSLLEAAADMACTLASADDSVHIIMKSFASNLGMAYQMIEDITADYS
                     RSVEDLDFDEQFITPTSRNSYTGLMGFDKTRTEVEKLLSEAEDLIKPFPHNEALREFV
                     QMIQERIP"
     gene            complement(6607..7635)
                     /locus_tag="PK015_04815"
     CDS             complement(6607..7635)
                     /locus_tag="PK015_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOL33821.1"
                     /translation="MEKLRLGLIGCGRISKNHFEAISQIPEAEYVAVADIIRERAEEA
                     AKTYNIPSVYTDYHKMLDKEQLDAVSICTPSGLHPSIGIDVANHKINVITEKPMAINV
                     EGADKLIHACDENNVQLFVVKQNRLNTTMQLLKRAVDKGRFGRIYMAQSNVFWQRPQS
                     YYDAAKWRGTWEFDGGAFLNQASHYVDALYWLLGNVGSVMAYTATMARHIEAEDTGCA
                     ILHFRSGIIATLNVTMLTYPKNFEGSITIIGEKGTVKVGGVAVNKIEKWEFEEYDDDD
                     RIVEEANYQPPNVYGFGHNPYYRNVVDVLLKRAEPLTDGRDGRKSVEIVQAIYLSAKT
                     GKRISLPL"
     gene            complement(7636..8640)
                     /locus_tag="PK015_04820"
     CDS             complement(7636..8640)
                     /locus_tag="PK015_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOL33822.1"
                     /translation="MNILVTGSNGFVGSRLMWELEKLNHKVIGIDISSHCDAKPHPET
                     VIGDIRSGKDLEKVQDIFYQKYNRPIELIIHCAASKHDFGISREEYFSHNQYGTQVLL
                     EFAEQADIHKIIYISTVSVFGHPQGRADEDTPYNPDHPYGESKLGGELLCIEWQKKNP
                     ENELIVLRPTAIYGPHNFANIYKLIDTLHRTPLFTVGKGDYVKSIVSLDTVIDMILFS
                     MSLLKPGYQHFNCVDEPYITLNELMHIIAANPGFHMPKITIPDWLAIFIGKLFDIPAK
                     LFSIDIPINSDRMRKLSMATNFCSQKIRQAGFVQRKSIEESISEMCTWYLDYIKNKEK
                     "
     gene            complement(8824..10050)
                     /locus_tag="PK015_04825"
     CDS             complement(8824..10050)
                     /locus_tag="PK015_04825"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOL33823.1"
                     /translation="MIIRTCGVQSKNKKDMQERKKKIVRIISRLCISGTSVHVSLLSY
                     HFNNDNWETVLLSGVPGENEGDMIYLPKQYGIEPIIIKHMGREISLWDDFLAEIQLIK
                     ILKKEKPDIVHTHTSKAGTLGRLAAILTGVPQIYHTFHGHTFEGYFSPLKTKFFIQIE
                     RFLARFSTKIIAISERQKEDLIRFKIAKSDKITIIPLGFDFSRLLPYDNEFKLRKKLN
                     IDKDKITIALIGRVTAIKNPALFIYSANEVLKQRNNVHFLIIGDGELTDDCKKLVNQL
                     GIQDYFTFPGFIEDLKLIYGSVDIVCLTSINEGTPVSLLEAMASQKIVIATPVGGVPD
                     FVKNGENGFLCDANPQAIAEQIIFCVDNFQNLDYLRKQAAEEVLSKYSMNRLFKDLEN
                     LYNSTPSKKSPNLFND"
     gene            complement(10019..11119)
                     /locus_tag="PK015_04830"
     CDS             complement(10019..11119)
                     /locus_tag="PK015_04830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOL33824.1"
                     /translation="MTHLIFCSTSIDCGGAESLLLNLVKELDNKYKIIIIYIIGKGTY
                     KSELKSTGAEVFKLNPSSLFRTIKLLYKNPDTILQGWMYHGNLLATFLYLLTLGRGKL
                     LWTVHHSAENYVHESLKHFLILKFTALLSRLPKAIVFVSEFIKKEHISYGYKNNHMQV
                     IYNGIDTSLFKRNEEAAKKLIYSLNIPSEAIIMGTVGRNHPVKDYRTFFRSSSLLIKK
                     HPNLHIIVAGRDVNLSQFSNELRDLTSEQLNRIHLLGERKDIPEILSLIDIFVLTSIS
                     ESFSISLIEALAAGCCCVSTNIIFYTHLFPEALTVFPPYDYQSLVKCVEKYIYMSKEQ
                     RNKIGKEASLKVEKYFSISKTTNDYKNLWSSV"
     gene            complement(11120..12238)
                     /locus_tag="PK015_04835"
     CDS             complement(11120..12238)
                     /locus_tag="PK015_04835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOL33825.1"
                     /translation="MDLINMNCIMHIPWKMIDESVAASEIRPRKMKKAFEDTGYNVFF
                     ITGTAAERNRQIKELKRRIELGENYDFLYSESSTLPMQLTESHHLPTHPLTDVKLFAL
                     AKRKGIPIGLFYRDFYWAFPEIHRFGKIKTYYANFFHKLDLKIYNHYLNIIYFPGDQY
                     DKLLKKIKYLDDKLEFHFLPSGVDLSYQHYDSPNNYFAYIGEIEPGRHNLKVLMQTFN
                     LFPQYELRICIPEKSWEKNKSYYEQFLSPNIKILHLVNEEAQDFLSYAKYAFFYFPDD
                     EYRNYALPYKLFEYVGHNLPIICNESDYSGALVKKLGIGYPIPHSVEALTEWLQNIPS
                     EEDYKQMCAHIELIKKENSWQKRAETVRDTLIKYRKRT"
     gene            12623..13972
                     /locus_tag="PK015_04840"
     CDS             12623..13972
                     /locus_tag="PK015_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOL33826.1"
                     /translation="MAGINLKKNILMGGSFRAIIMIFSFFCSLITARYLGVELKGEYS
                     YLVTVGGFLWMILDLGVYRSYPYLIRKYPDKIYNLFKWATLTFILEAIILSVLGISLL
                     KVWSKILDYNFKPEYMLLFIGYITLSKYFMQMQSIYLGMNKILIQSLGDILNSGLCLL
                     FFLLAFIFIKDGDRLSVIFIATLMAVAIAIIYFLINSKPRTLPKGLNFKFITSAYSYG
                     IRVFLSSIIIMLLIRVDIVLIKRMLGFREVGIYSIAAHIVDMLQVASNLVGGYLFVKL
                     SDTEDDFTKWQLMKKIMMLFGAFLTVANLAFVLLGKFLLRILYGIDFVPAYGVYLWLI
                     PASYGLSFGSFFNNYLNSKGFPPITILIPAISLGLNIGLNLLMIPRWGLIGAAIASGI
                     AYLLWFFLIIIYEQRRTKGAMLPVLIPSKQDWKDFWEEGKATLNTALSHLHKNRSKD"
     gene            complement(14224..15561)
                     /gene="gdhA"
                     /locus_tag="PK015_04845"
     CDS             complement(14224..15561)
                     /gene="gdhA"
                     /locus_tag="PK015_04845"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOL33827.1"
                     /translation="MTFQEFMAKVEAKNPGEPEFIQAVSEVVESIWDVYESNPRFVKA
                     NVLERLVEPERVIIFRVPWMDDKGEVHVQRGYRVQFNSAIGPYKGGLRFHPSVNLSVL
                     KFLGFEQIFKNSLTSLPLGGGKGGSDFDPRGKSDEEIMRFCQSFMAELFRHIGPNTDV
                     PAGDIGVGAREIGYLFGYYKKLMNEFTGVLTGKGINWGGSLIRPEATGFGIVYFTEEV
                     LNTIGETIKGKRVAVSGFGNVAWGAVQKVNDLGGKVVTLSGPDGYIYDEDGVSGEKID
                     FMVTMRNSNRDLVQDYADKYPSAKFFPGKHPWEVPVDVAIPCATQNELNEQDAMNLIN
                     NKCICVVEGANMPCLPKAIEAFQKAKILFAPGKAANAGGVATSGLEMTQNAMHLSWPR
                     EEVDARLHEIMKNIHKACVEHGKEPDGYINYVKGANIAGFMKVANAMCDQGLV"
     gene            complement(15621..16844)
                     /locus_tag="PK015_04850"
     CDS             complement(15621..16844)
                     /locus_tag="PK015_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOL33828.1"
                     /translation="MKLEGKVVIAQGGGPTAVINQSLVGITLEARKFVQVTRVYGSLY
                     GVRGIVDEEFVDLTQETTHNLEQVANTPSSALLSTRDKPDEKYCKEIFKVLKAYDVRY
                     FFYIGGNDSADTVRIVNEEADKADYEFRAIHVPKTIDNDLVLSDHTPGYGSAARFVAS
                     AFTGVNLDNRALPGVYIGVVMGRNAGFLTAASALAQKYPDDGPHLIYMPERPFNQETF
                     LRDVQRVYDRYGRCVVAVSEGIVDENGVPMMTILSKNTEVDAHGNVQLSGTGVLGDLL
                     SDLVRTRLKIKRVRSDTFGYLQRSFIGCVSDVDQREAREVGERAAHYAIWYNLDGSIS
                     IQRTGFYSVNYQLENLSDIAGKTRHMPDEYINESGNGVTDAFKYYLRPLLGSGFPTAH
                     RLRAPKVDKILQVNH"
     gene            16994..18601
                     /gene="dnaX"
                     /locus_tag="PK015_04855"
     CDS             16994..18601
                     /gene="dnaX"
                     /locus_tag="PK015_04855"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOL33829.1"
                     /translation="MNYIVLARKYRPQTFAEVYAQEHITEVLQNAILSNRIAHAYLFT
                     GPRGVGKTSMARILAKSLNCVEGPTITPCNKCHNCVEIASGISADVIEIDGASNTGVD
                     DIRELQRELLYAPSQSPYKIYIIDEVHMLSKNAFNALLKTLEEPPDNVIFIFATTEPY
                     KVLPTIISRCQRYDFKRIPIDAIVKRVGEIMQEENIEIDAESLHLIARKADGSLRDAL
                     SLADQVLSYCGNKVTIDKVRDIFGLIPTQIYCNIMKLLHSKNNAGLLEQLQHIFEGGA
                     DLQEFINNLLEFLRIVLLRKIGMSPEEVTSEEYPAYDEIASLFSQENLLYMMSMLMQT
                     RTELKSSGNPYLIFELAMIKLCKLDEMEDLGEVIARLKSQPLTKVVPQTPKQPVSKGV
                     TTPVNIESKPDIITSAEPEPIDKKELTQENLTELWDKIVARVIQIGRMAGLALKEANF
                     KVSSGFKIIIQLSRKSSLDAVQGKKEDIEQIIGEYFQTQPRLELELISGNTVDSIVIP
                     HKTLEDLKKMDENLARYIEITDSKLIS"
     gene            complement(18830..>19124)
                     /locus_tag="PK015_04860"
     CDS             complement(18830..>19124)
                     /locus_tag="PK015_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HOL33830.1"
                     /translation="QRVAIARALMQEPELILADEPVASLDPATADSIMQYLGEINREG
                     VSVICSLHFLTLARRYGNRVVGLKQGIKVFEGHPEDIDKVRFKEIYGEEAEEI"
BASE COUNT         6164 a         3444 c         3348 g         6168 t
ORIGIN      
        1 gtattgaggt cttctatttt taagggattt ttgataccgt agattttcac accaccttgt
       61 cctaatgcta caaagagtcg gtcgttatag atttccaggt cctggatttc attagcaaga
      121 tacataaggt taatcagacg gggatgtttc atatcggcaa cattgatagc gaggagtttt
      181 tttcctgcac caatccagat gatattgcca tatttagcca gaaccgtggc atcagcgatt
      241 tcataattga caccaagact gatgggaaac tggaggtcgg agatatctat ggttacaagt
      301 ccaaaatcgt tcaaggcata gatataggga tattctgcca gaaggtcgcg aataccccat
      361 ttttcagaaa aggtggactt gagttcccgc attcctcctc ttccgatgtc aatgatatca
      421 atacctcgga acctatcagc cacatagaga atagagcctt ctcgggcaat tttattaccg
      481 ataacagtag tggtgaagaa tattttccct ggataggaga gagtatccac agaagtgacg
      541 gtattggaag gttcttcact gcagacatag agatgattat ctcccaaaag gttgaggtct
      601 tcaatcaaag cggaagaagt gaaaacagct tccaaacgag gtgaccaagg attgaatatg
      661 gaataaatcc agataaagtt ttgattacgg agaataacta aatcttgctg ataatccaca
      721 ttatagggtt gaatattttt tagatgcact aaaggacgag gaatacggtc atcaatagca
      781 tagccggaaa ccagtatggt cattatcagc agtaaaagta gagctttttt cattattcac
      841 tccttccagg ttgaaaaacc tgtagcaata acaattgctt tttctttata cttataggga
      901 agagattatc ctgcattaag aaagcagttg cttgatgata tcggaaggtt taattccttc
      961 tgcttcagcg gcaaaggaaa ccaaaacccg atgcgtgagt acaggtaaag ctacagcgcg
     1021 aatatcttct tcggcaggag tcaatcttcc attaagagca gcccgagctt ttgctgctaa
     1081 aacaagatat tgactggcac gaggtcctgc accccagttt atccattttg ttatttcagg
     1141 tctactttct ggtgttccag gacgtgtgct acgagcaagt ttaacagcat attccaggat
     1201 atgttggcta accggcatag ttcttatagc cgcttgatat tggagaattt tttctgcaga
     1261 aagtaaaggt aaaggctcct cttgcgggac tcctgtagta ttttgcacaa tggtcaattc
     1321 ttcttcataa gaaggataat caatattgat ataaagcata aaacggtcta attgcgcttc
     1381 cggcaaagga taggttcctt cctgttcaat aggattttgg gttgccatca caataaaagg
     1441 taaatccaga ggtctggtgg tatttccgct acttacttga tattcctgca tagcttgaag
     1501 caaagcagct tgagttttgg gaggggttct attaatctca tctgccagaa ttaggttagc
     1561 aaagatagga ccttttatat attgaaattc acggcgttca ctatctcggg atgtgactaa
     1621 gatatcagaa ccggtgatat cagaaggcat aaggtcggga gtaaattgaa tgcgtccgaa
     1681 ggttaaattt aaagctttgg caatagttga aataagtagt gtttttgcta atccgggcac
     1741 accttcaatc aacatatgtc cattagcaaa cagtccaatg aggaccttgt ctataacttc
     1801 atcctgaccg acgatagttt tgcgaatctg agtgagtaaa tcctctttta cctgttttaa
     1861 ttcctgaatt ttttgaatca tttcttcact gttcataaat cttttctcca cttaataccg
     1921 cttttagaac ctgacgacca ctaagggcaa tagagaccag tccaataact ccaatcagga
     1981 tggcggttaa catatgtgca aaagccataa ttgctgatgc gtcctctctt gtcaatgcaa
     2041 aacccacgga aaagatgatt atcatcatcc attcgttgct acctaattga gctggaggtt
     2101 gaggtatggc ataagaaaga ttgattaaag tatagccaaa aaggactttt aagaaaggat
     2161 aatcaatgcc aaaagcaaga aataggagat agaaatataa gccatcgaga aaaacaccca
     2221 aagcagtaag tagaaaagaa ttgagtaaac gcgtccagtg atgttcaaaa atattcaaac
     2281 cttggacaaa gatgctgata taattattga ctttagtctt gagtttcttt ggtaaccagg
     2341 aaaataaaac ttgaaggaat ctaaccactt tatctttttg ccagactgat aataatagaa
     2401 cagccagaga aacagcaaaa accagaccca gaagtataag taaaataatc agaccctgcg
     2461 agaatttaac ttttagaagt ggtatcatta agaggacaaa gaatattcca agagtgtcaa
     2521 aagtcttatc aatgaagatg gatggtagca cgctggtaat agaattcccg tgattgtgct
     2581 taacaaacca ggctttgact aattctcctg ctcggatagg aataagataa ttaacccagt
     2641 ttccacccat actataaaga taggttttcc agatagaaat tgaggtctgt ttgctcaaaa
     2701 ggatattcca acgcacacta cgaatgaaat aagctgccaa ataggaaata gtagcccaga
     2761 aaactaagcg ggtatcaatt cttttcatcc gagctacaag ttcattaagg tcaatataat
     2821 gcaaccatag aatgatgagc acaatgccta taattaagcc aataatcaga tatagaattc
     2881 gtttacgaga catagtttat tgccagcgat attttttgca catctaaaac aatttcagct
     2941 ttaaaaagaa ttcagggtgc aaaaatcata taataatgat gagcccaaac ctttttaaaa
     3001 gcatcaggag cagtcttttc cagccattgg taacgacgca tccttggagc aaaatcaggg
     3061 tctttaccag aataataatc caaaatagag gatgattctt tatccggttg ttccttaaag
     3121 atttttgaga taggaagttg aggaaaccat ttttttaaaa gctcttcttt agtcatacct
     3181 atatcaggcc agggtggaca atcaaaatat cctgagtcca ccagatacca gtctaatttc
     3241 ttgagaatat gaataatgga aggtgggtct atgaaactga gtttgatttg aggatatttt
     3301 tctggagtat aatcctttat ctgcatttta aaaccaagac cattacgatt ggggatactg
     3361 ataaagataa cttgagaggt aactctggat aattctttaa ggaagtggga taaatcagga
     3421 acaaaccaga gagcagaaaa agagaaactc atatcaaagg atttatccgg ataattaagt
     3481 tcgtggaaat ctggattata aaccggatta aaattgcgac caagcttttt ccaaaggtct
     3541 gtaattaagt ccaatctatg taagtcatta tcttccaggg tgatattcaa tcccaaatca
     3601 gccagattca aaagattgat gccacttata cccgtaaaac caaaggaagg actttccagc
     3661 acattatgga cattataatt atttaccgtc tgaaataaaa ggtcattgag gacaatccgt
     3721 tcataggaag aacccaatcc ttcataagga ttagagaaga acttttccca gtcgttaata
     3781 attggaatag ccattatttc ttcaagatat catcaatgat gatgcgaata tcaggaatgg
     3841 taaaagtggg aggaaggttc ataagttcat aggtgttatc cacataatag aaccaggaat
     3901 gagaaataag ttgaaagcga ctggtccaga gttcattttt caatttacgg gcaatagatt
     3961 cacctaaagc aaaaagattt cggtctaatt ttaaaatagt attatagttc ttgccgtgaa
     4021 tttcacgaga gataaaatcc accagattcc ttaactgaac aggttcacga tctgccacat
     4081 taagtgccaa tcctggagta tattcatttt ttaaaagcca gaggaaagca cgggttatag
     4141 tattcacatt gcaaagatga atccagatgc gtttattgat aatagggaaa agcttttttt
     4201 gaaccagctt caccatctga taagggaaac cataatcccc tggaccataa gtgatagaag
     4261 gacgaaggat ggcagcattt agacctttta atacggcttg atttattatt ctttcacttt
     4321 ctatcttagt ccagtgataa aggctatcag gattgcgttc cgtttcattg gtggcaggta
     4381 gttctttggg tataactcca aaaactccca cagaagaaca ataaatgaac cgagaaccgt
     4441 gtttttgaca atgttcaaca aattgctccg tgctataata gttagcttta atatagatat
     4501 cacgagggaa tttgcgtccc cctctcagag cacctatatg cattactaca tcaaaactat
     4561 ttttcttcag ataatcataa agtctttctg tatcggctaa atcaatctgt gccagctcaa
     4621 cagaacttct aaaagatgag aaccgctcta aagcagtgtt tggtctaatg agagcggtaa
     4681 tttcataatc cagttgttct tttaaaatag aatttaggac agatctgcca attaagcccg
     4741 ttacacccgt tatcagtaat ctttgcattt aaccaaaaat cctttcccag aaagatttat
     4801 gatgatggtg atgatgatgc gaagactgta tatatacata gaatagaggt atctgctcat
     4861 cattaataat catttgggga aaatccagag tgagtaattg aaccgtatgt tcatcaatcc
     4921 tttctttaat tagctttatg gcttgaggga ataaattatt gggttcattg gcatgactat
     4981 cagaacccag cagatgtgcc caaccgttat ctaaaagtac ccaagcagtt tggcgaacct
     5041 tttctccata ttttcctaaa agactgccag cattaatctg gagatagata tctttttcca
     5101 tcagctcttt ggcattatga ggtttatgca taatgcttac atacctttca gcgtgtgcca
     5161 gaactggacg atagccattt ctcaaaaggg gatagatatt ttcataaaag tccttgggaa
     5221 gaccatttaa atctgattca atcaacacat aatgagagtt tccgagagtt agattaaatc
     5281 tttcaatatc agttaaagaa gtaggattga ggagaacttc aaaaccagga tataaagtaa
     5341 tatttattcc ttctttctgg acttgggatt gcagctcttc aaatctatca tcatataaag
     5401 cacggtcata ttgataaaga ccaggaagat agtgtgaagt aagaaaagcg tgagttatgc
     5461 catcctccac catctgccgt aaaagcttta gagatgtttc aaaggaagaa gacccatcat
     5521 caatattcgg gactaaatga caatgtgtat ctatcatagc tcagggtatc cgttcctgaa
     5581 tcatttgcac aaattccctg agagcttcat tatgaggaaa tggttttatc aaatcttccg
     5641 cttcggaaag aagtttttcc acctctgtcc gggttttatc aaatcccatc aaaccagtgt
     5701 aactatttcg tgaagtaggt gttataaatt gctcatcaaa atccaggtct tctacactac
     5761 gagaataatc agcagtaata tcttcaatca tctgataagc cataccaaga ttagaggcaa
     5821 aggatttcat aattatatgc acgctatcat ctgcactggc aagagtgcaa gccatatcgg
     5881 cagcagcttc taaaagagag cccacttttt tcaaatctat ataatgcaga gtattcattt
     5941 tcattacttt gtgcttactt tctaaagcta ctgcctgtcc tccaattaaa ccgaaagaac
     6001 ttgcactatt agacaagata cgaatacagc tcagggcttt agaaggaggg gaaatttgtc
     6061 ctaaaacctc aaaagcaagg gtgtaaagtg catcaccagt aagaatagca gtagcatttc
     6121 caaatttggt atgcaacgca ggttgagagc gccgctctgt tctattcatt attataggta
     6181 aatcatcatg caccagtgaa gcattatgaa ccagttccac agcagtggct gcttttaaag
     6241 cctggggtaa accagtattt tttcgcattc ccattaacat ttcatatata gaaagcaaaa
     6301 gcaaaggacg ccagcgcttt ccgccagaaa aaacagcata atgcatagct tgttttaact
     6361 gtttattcgg tccagattga gttcccagat attccttcaa gctggtttca atcatatcta
     6421 atctactttc aatatatttt tgtaatagca agttattctc ccgaaggctg ttactttgat
     6481 ttgtctatta ttataaattc attaaacgat aaagggtatt attgtcaagt tttttgttcc
     6541 ggttttaatt aaaaaattag ggagattgta aaatctccct gttttgctat atataaaatg
     6601 attggattat aaaggtaggc ttattctctt acctgtttta gctgaaagat agatagcttg
     6661 aacaatttca acggacttgc gtccatcacg accatcggtt agaggttctg ctctttttag
     6721 aagaacatcc accacattcc gataataagg attatgcccg aatccatata cattaggggg
     6781 ttgatagtta gcctcctcca caatacggtc atcatcatca tattcttcaa attcccactt
     6841 ttcaatctta ttaactgcca ctcctcctac tttaaccgta cctttttcac ctataatagt
     6901 gatagaacct tcaaagtttt tgggataagt gagcattgta acattcaaag tggcaatgat
     6961 tccggaacgg aaatgcaaaa tggcacaacc agtgtcctct gcttcaatat gacgagccat
     7021 cgtggcagtg taagccatca cactgccgac atttcctaaa agccagtaaa gagcatctac
     7081 ataatgactt gcttgattta aaaaagcacc accatcaaat tcccaagttc cacgccattt
     7141 agcggcatca taataagatt gaggtctttg ccagaataca ttggattgag ccatataaat
     7201 acggccaaaa cgacccttat ccactgctcg tttaagtagt tgcatcgtgg tattgagacg
     7261 gttttgtttc actacaaaaa gctgaacatt attttcatca caagcgtgaa taagtttatc
     7321 tgcaccttcc acattgattg ccataggctt ttcagtaatt acattaattt tgtgattggc
     7381 aacatctata ccaatggaag gatgaagacc actcggggtg caaatgctaa ctgcatcaag
     7441 ttgttcttta tccagcatct tatgataatc cgtataaact gaaggaatat tataggtttt
     7501 agcagcttct tctgctcttt cacgaataat atccgctacc gctacatatt ctgcctccgg
     7561 aatttgtgat atagcttcaa aatgattttt agagatgcgt ccacatccta ttagacccaa
     7621 ccttaatttt tccatctatt tctccttatt tttaatataa tctaaatacc aagtgcacat
     7681 ttcggaaata ctttcttcaa tgctctttct ttggacaaaa cccgcttgcc tgattttctg
     7741 agaacaaaaa ttagtcgcca ttgataattt tcgcattctg tcgctattga ttggaatgtc
     7801 aatagaaaaa agtttcgcag gtatatcaaa caatttacct ataaaaattg ctaaccaatc
     7861 cgggatagtt atctttggca tatgaaaacc aggattagca gctatgatat gcataagctc
     7921 attcaaagtt atataaggtt catccacaca gttgaaatgc tgataaccag gcttaagtaa
     7981 gctcatagaa aaaagtatca tatcaattac ggtatccaga gaaacaatgc ttttaacata
     8041 atcgcctttg cccacagtga acagaggtgt gcgatgaaga gtatctatta atttataaat
     8101 attagcgaaa ttatgaggtc cataaatagc agtgggacga agaacaataa gctcattttc
     8161 aggattcttt ttttgccact caatacataa caattctcca cccagtttgg attcaccata
     8221 tggatgatct ggattatagg gagtgtcttc atcagctctg ccttgaggat gtccaaaaac
     8281 ggaaactgta ctaatataga taattttatg aatgtcagct tgttcagcga attctaataa
     8341 tacctgagtt ccatactgat tatgactgaa atattcctct cgggatattc caaaatcgtg
     8401 ttttgatgct gcacaatgaa ttatcaactc aattggacgg ttatattttt gataaaagat
     8461 atcttggact ttttccaaat ctttaccgga acgaatatct cctataaccg tttctggatg
     8521 aggttttgca tcacaatgag aacttatatc aattcctatc accttatgat ttaacttttc
     8581 caattcccac attaagcgag aacctacaaa accattactt ccagtaacca gtatattcat
     8641 tggatattat ctcctcttta gttttaaccc actttaaatc ttataatttg gaaatctttt
     8701 tgaataaatt aatgttcaat ataatctcct tctaccctta aaaagagatt gaatataaac
     8761 tgtcaattta aaagaatccc agtaataatg gtaagaaaaa tcttagaatt tctgcagtct
     8821 tcattaatca ttaaaaaggt taggtgattt tttagaagga gtgctattat ataaattttc
     8881 taaatcttta aataagcgat tcatactata tttggaaagc acctcttcag ccgcttgctt
     8941 tcttaaatag tcaagattct ggaaattatc cacgcaaaag attatttgtt cagcaatagc
     9001 ttgtgggtta gcatcacata aaaaaccatt ttccccattt tttacaaagt ccgggactcc
     9061 accaactgga gtagcgatta caatcttttg agaagccata gcttccagaa gggagacggg
     9121 tgttccttcg tttatagagg ttaggcacac aatatccacc gagccatata ttaattttaa
     9181 atcttctatg aaacctggga aagtgaaata atcttgtatt cctaattgat ttaccagctt
     9241 cttacaatca tcggttaatt ctccatctcc tattatgagg aaatggacat tatttctttg
     9301 tttcagcact tcgtttgcgc tataaataaa aagagctgga ttttttatgg ctgtcactct
     9361 acctatgagg gcaatagtaa ttttgtcctt atctatgttt aacttttttc ttaatttgaa
     9421 ttcattatca tagggtaata atctggaaaa atcaaaacca agaggaataa tagttatttt
     9481 atcagattta gcaattttaa atctaatcag atcttctttt tgtcgttctg aaattgcgat
     9541 gattttagta gaaaatcggg caagaaatct ttctatttga atgaaaaatt tggttttaag
     9601 aggagagaaa tatccttcaa aagtatgacc atgaaaagta tgataaatct gaggaactcc
     9661 agttaaaatt gcggctaacc gacctaaagt cccagcttta gaggtgtgag tatgcactat
     9721 atcaggtttc tcttttttta gaatttttat taattgtatt tctgctaaaa aatcatccca
     9781 tagtgaaatc tcccgtccca tatgtttaat aattatgggt tctatgccat attgttttgg
     9841 caggtaaatc atatctcctt cattttcacc aggaacccct gacaataaaa ctgtctccca
     9901 attatcatta ttaaaatgat agcttagtaa gctaacatga actgaggtac cactgataca
     9961 tagccttgaa attattctta caattttctt ttttctttct tgcatatctt tcttattttt
    10021 agactgaact ccacaagttc ttataatcat ttgtggtctt agaaatagaa aaatattttt
    10081 ctaccttcaa acttgcttcc ttaccaattt tattgcgttg ctctttggac atatagatat
    10141 acttttctac gcattttacc aaagattgat aatcataagg tgggaaaacc gttaaagctt
    10201 ctgggaaaag atgtgtgtaa aaaataatat tggtacttac acaacaacaa ccagcagcta
    10261 aagcttcaat aagagaaatg gaaaatgatt cggaaataga ggttaaaaca aatatatcaa
    10321 taagagaaag aatttcaggt atatccttcc tttctcctaa taaatgaatt ctatttaatt
    10381 gctcagaagt caggtctctt agttcatttg aaaattgaga gagattaaca tctctacctg
    10441 caacaataat atgaagattt gggtgctttt taataagcaa agaggaagat ctaaaaaagg
    10501 tcctgtaatc ttttactgga tgatttctgc ctactgttcc cataattatt gcttcagaag
    10561 gaatatttaa tgaatagatg agttttttag ctgcttcttc atttcgttta aataaagaag
    10621 tatctatacc attataaata acctgcatgt ggttgttttt ataaccatag ctaatatgtt
    10681 cctttttaat aaattcggat acaaatacaa tagctttagg tagacgagat agaagagcag
    10741 tgaattttaa aattagaaaa tgttttaaag attcatgaac ataattttca gctgaatgat
    10801 gaactgtcca caaaagtttg cctctaccca atgtgagcaa atataggaaa gttgctaaca
    10861 aatttccatg atacatccat ccctgtaaaa tcgtatctgg atttttatat aataatttaa
    10921 tggttctaaa taaagaactt ggatttaatt taaagacttc agcaccagtt gattttagct
    10981 ctgatttata tgttcccttc ccaataatat aaataataat aattttatat ttattatcaa
    11041 gttcttttac gagatttagc agtaaagatt cggctccccc acagtctata ctggttgaac
    11101 agaaaattaa atgtgtcatc tatgtccttt ttctatattt aataagggta tcacggactg
    11161 tttctgctct tttttgccag ctattctctt tttttatcaa ttctatgtga gcacacattt
    11221 gtttataatc ttcttcagag ggtatatttt gcaaccattc agttaatgct tctacagaat
    11281 gaggtattgg atagccgata ccaagttttt ttaccaaggc tccagaataa tccgattcat
    11341 tgcatataat cggtaaatta tgtcccacat attcaaataa tttataggga agagcataat
    11401 ttctatattc atcatcagga aaataaaaaa aggcatattt agcataagaa aggaaatcct
    11461 gtgcctcttc attgactaaa tgtaaaattt taatattagg actaagaaat tgctcataat
    11521 atgatttatt tttttcccaa cttttttcag gaatacagat tcttaattca tattggggaa
    11581 atagattaaa agtttgcatt aaaactttaa gattatgcct gccgggttca atctctccga
    11641 tataagcaaa ataattatta ggagagtcat aatgttggta ggacaaatca acacccgaag
    11701 gaagaaaatg gaactctaat ttatcatcta aatatttaat cttttttaat agtttatcat
    11761 attgatcgcc aggaaaatag attatattta gataatgatt atatattttt aaatcaagtt
    11821 tatggaaaaa attagcatag tatgttttga tctttccaaa acgatgtatt tccgggaaag
    11881 cccaatagaa atctcgataa aataatccta taggaattcc ctttctttta gctaatgcaa
    11941 atagttttac atcagttaag ggatgagtag gaagatgatg agattcagtc aattgcattg
    12001 gtaaagttga agattcacta tataaaaaat catagttttc tcctaactct attcttcttt
    12061 ttaattcttt gatctgtcta tttcgttcag ctgcagtacc tgtgataaaa aaaacattat
    12121 aacccgtatc ttcaaaagct tttttcattt tgcgagggcg aatttctgaa gctgctactg
    12181 attcatcaat cattttccag ggtatatgca taatgcaatt catatttatt aaatccatta
    12241 ttaaatattt atctcatctt atacttatca tgttacatta tttgtgttgc aatgttatgt
    12301 gagacatagc taagacttta ttttgaatca tttagtagat caaaacttat aattttctat
    12361 gatattatat cctatcataa ttgtcaagac aaaattagaa cataaatttc tatctttttc
    12421 ttgggtaaaa attcatgggt tattcattga aattaaaact tccaaccctc gttctaatat
    12481 agaaatttca tagaatctat ctttaaaaga aagcatggaa taagaaatat ttgacagtca
    12541 atatgctttt ttaattgtga acttaatcta ataatctaaa aactataata gactaaacca
    12601 tcctaaaggg agtaaaaaat agatggctgg aattaatctg aaaaagaata tcttgatggg
    12661 aggaagtttc cgtgccatta taatgatttt ttcctttttt tgtagtttaa taactgcacg
    12721 ttacttaggt gtagaattaa aaggtgaata cagttatctt gttaccgttg gtggtttttt
    12781 gtggatgatt ttagatttag gagtttatcg tagttatcct tatttgatta gaaaatatcc
    12841 agataaaatt tataaccttt ttaaatgggc aacacttacc tttattctgg aagctataat
    12901 tctatcagtt ctgggaatat ctttgcttaa agtctggagc aaaatattag attataattt
    12961 taaacctgaa tatatgttac tttttatcgg ttatattaca ctatccaaat actttatgca
    13021 aatgcaaagc atatatttgg ggatgaataa aatattaatc caatctttag gagatatatt
    13081 aaactctgga ttgtgtttat tattttttct tttagccttc atttttataa aggatggaga
    13141 tcgattatca gttatcttta tagctacatt gatggcagta gctattgcta taatttattt
    13201 ccttattaat tctaaaccga gaactttacc aaaaggttta aactttaagt tcattacttc
    13261 tgcctatagt tacggtatca gagtatttct gtcttctata ataattatgt tattgatcag
    13321 ggtagatatt gttttgataa agcggatgtt gggtttccgt gaggtgggaa tttattctat
    13381 tgctgctcat attgtagata tgttgcaagt tgcttccaat ttagtgggag gttatttatt
    13441 cgtaaaactt tctgatacag aggacgattt tactaaatgg caattaatga aaaagattat
    13501 gatgcttttt ggggcttttt taactgtcgc taatcttgct tttgtgcttt taggtaaatt
    13561 tctattacgt attctttatg gtatagattt tgttcccgct tatggtgtat atttatggtt
    13621 aattccggca agttatggac ttagttttgg ttcatttttc aataattatt tgaacagcaa
    13681 gggatttcct cctattacta ttcttatacc agctatttca ctgggcttga atataggatt
    13741 aaatcttcta atgatacctc gttggggatt aattggtgct gctatagcta gtggtattgc
    13801 ttatttatta tggtttttct taattattat ttatgagcaa agaagaacaa aaggtgcaat
    13861 gttgccagtg ctcatacctt ccaaacagga ttggaaagat ttctgggaag aggggaaggc
    13921 tactttaaat acagcacttt ctcatcttca caaaaataga agcaaggact aagatgagaa
    13981 aattgtcaat aagaataaag cctcattttc taagctacta agtattaaac tgtacaatta
    14041 ataagtaatt aagtttacat tcattctttt aggtaagatt gttagcaatg aaatgaaagc
    14101 atagaatagt agtgatttta gtaacaaagt attaagattc tgagatgaat ttctgagttt
    14161 ctacgggaaa atataagacc ctgctcagtt gaagcagggt cttattgttt aattgttatt
    14221 tatttagact aaaccttggt cgcacattgc attggcaact ttcataaagc cagcaatatt
    14281 agcacccttc acataattta tgtatccatc aggctctttt ccatgttcca cacaggcttt
    14341 atgaatattc ttcataattt catgcaaacg agcatcaact tcttcacgag gccaggaaag
    14401 gtgcattgca ttctgggtca tttccagtcc actggttgcg actcctccag catttgcagc
    14461 cttaccaggg gcaaagagaa tttttgcttt ctggaatgct tcaatagctt taggaaggca
    14521 gggcatattt gcaccttcaa ccacgcagat acatttgtta ttaatcagat tcatagcatc
    14581 ttgttcattc aattcatttt gcgtggcaca ggggatagct acatcaacag gaacttccca
    14641 aggatgtttt ccagggaaga atttagctga aggatatttg tcagcataat cctgaaccag
    14701 gtctctatta gaattacgca ttgttaccat aaaatctatc ttttcaccag aaacaccatc
    14761 ttcatcatag atatatccat ctggtccaga gagggtaacg accttcccgc ctaaatcatt
    14821 aactttttgt actgcacccc aggcaacatt tccaaatcca gagacagcaa ctcgttttcc
    14881 ttttatggtc tctccaatag tgttaagaac ttcttcagta aaatagacaa ttccaaaacc
    14941 agtggcttca ggacggatga gactaccacc ccaattaata ccttttccag taagaactcc
    15001 agtaaattcg ttcattagct tcttgtaata tccaaaaaga tatccaattt cccgggcacc
    15061 aactccaata tcaccagcag gtacatcagt attaggtcca atatggcgga aaagttcagc
    15121 cataaaagat tggcagaaac gcataatttc ttcgtcagat tttccgcgag gatcaaaatc
    15181 tgaaccacct ttaccaccac ctaagggtaa ggaagttaaa ctgtttttga atatctgttc
    15241 aaaaccgaga aacttgagca cggatagatt aacactggga tgaaatctca aaccaccttt
    15301 atagggtcct atagcactat taaattggac acgatagcca cgttggacat ggacttcacc
    15361 cttatcatcc atccagggaa cgcggaaaat gattacccgt tcgggctcaa ctaaacgctc
    15421 taatacatta gcttttacaa aacgaggatt gctttcatag acatcccata tggattctac
    15481 tacttcgcta actgcctgaa taaattctgg ttcaccagga ttcttggctt ccacttttgc
    15541 cataaattct tgaaaggtca tttcttcctc cattttatta ttttatattt ttatccattt
    15601 aaaattacat tataaaaatg tcaatggttt acctgcaata tcttgtctac tttaggtgcg
    15661 cgaagacggt gtgcagttgg aaatcctgag cctaaaagtg gacgaagata atatttaaat
    15721 gcatccgtca ctccatttcc actctcatta atatattcat ctggcatatg acgagtcttt
    15781 cctgctatat ccgaaagatt ttctaactga taatttactg aatagaatcc agtgcgttgt
    15841 atggaaatag aaccatccag attataccag atagcataat gagcagctct ttcaccaact
    15901 tctcttgcct ctctttgatc tacatcagaa acgcatccga taaaggaacg ctgaagataa
    15961 ccaaaggtat ctgaacgaac tctctttatt ttgagtcggg tgcgaactaa atcacttaat
    16021 aaatctccca aaactccagt tccagaaagc tgaacattgc catgagcatc tacttcggta
    16081 tttttactta agatagtcat catagggact ccattttcat ccacaattcc ttctgaaacg
    16141 gcgactacac atcttccata gcggtcatag accctctgca catcacgtaa aaatgtttct
    16201 tgattaaatg gtctttccgg catatatata agatgtggtc catcatcggg atacttttgt
    16261 gctaaagcag aagcagcagt aagaaatcct gcatttcttc ccattaccac acctatatat
    16321 actccaggta aggcacgatt gtcaagatta actccagtga aagcagaagc tacaaaacgt
    16381 gcagcagaac cataaccagg ggtgtgatca cttaatacca ggtcattgtc tatggtttta
    16441 ggaacatgaa ttgccctgaa ttcataatct gctttatctg cttcctcatt aacgatgcga
    16501 accgtatcag cagaatcatt tcctccaata taaaagaaat agcgaacatc gtaagctttc
    16561 agcactttaa agatttcttt gcagtatttc tcatctggtt tatcccgggt tgaaagtagg
    16621 gcagaagaag gggtattggc aacttgttcc agattgtggg tagtttcctg agtaagatca
    16681 acaaattcct catcaacaat tccccgaact ccatataaag aaccatagac tcgggtgacc
    16741 tgaacaaact ttcttgcttc cagagttata cctactaatg attggttaat gacagctgta
    16801 ggtcctcctc cttgagctat aaccactttg ccttccaatt tcattggcta acctccagtt
    16861 tttctataaa ttataatagt atttataaac agaaaagtat gaaaaccttt ttatctttgc
    16921 gcacttaatt gtccagaaga aaatgcttgc ctaaatgtaa ggactgcaaa actaggtata
    16981 tggagtttaa tttatgaatt atatagtact tgcaagaaaa tatagacctc aaacttttgc
    17041 cgaagtttat gcccaagagc atattactga ggttttacag aatgctattc tgagtaatcg
    17101 tattgcgcat gcgtaccttt tcactggacc tagaggagta ggaaagacct ctatggcaag
    17161 aattctggct aaaagtctga attgtgttga aggtccaact attactcctt gtaataaatg
    17221 ccataattgt gtggaaattg cctcgggtat ttcagcagat gtgatagaaa ttgacggtgc
    17281 ttccaacaca ggtgtagatg atattcgtga actacaacga gaacttcttt atgctcccag
    17341 tcaatctcct tataagattt atattattga tgaagttcat atgctttcca aaaatgcctt
    17401 caatgcactt ttgaaaacat tggaagagcc tccagacaat gttatcttta tctttgctac
    17461 tactgaacct tataaagtat tgccaactat catttctcgt tgtcagcgtt atgattttaa
    17521 gcgaataccg attgatgcta ttgttaaaag agttggtgaa attatgcagg aagagaatat
    17581 tgaaattgat gcagaatctc tccatttaat tgccaggaaa gcagatggaa gtttacgaga
    17641 tgctctatct ttagctgacc aggtgctctc ctattgtgga aataaagtta ctattgataa
    17701 agttcgtgat atctttggct tgattcccac tcaaatctat tgcaacatta tgaaacttct
    17761 ccattcaaaa aataatgcag gcttattaga gcaacttcag catatctttg aaggtggggc
    17821 agacttacag gaattcatca ataatctctt agaattttta aggattgttc ttttaagaaa
    17881 gattgggatg tctcctgaag aagttaccag tgaagaatat cccgcttatg atgagattgc
    17941 cagcctcttc agtcaggaaa atttgctcta tatgatgagt atgcttatgc aaacaaggac
    18001 agaacttaaa agcagtggca atccctattt aatttttgaa ctggcaatga ttaagctctg
    18061 taaacttgat gaaatggagg atttagggga agttattgct cgtctaaaat cgcaacctct
    18121 gactaaggtt gttcctcaga caccaaaaca accagtttct aagggggtta caactcccgt
    18181 taatatagaa agtaagccag acataattac ctcagcggaa ccagaaccta ttgataaaaa
    18241 ggaactaacc caggaaaatc tcacggaact ctgggataaa attgttgctc gggttattca
    18301 gattggtaga atggcaggtt tagctttgaa agaagcaaac tttaaagtta gcagtggctt
    18361 caaaatcatt atccagttat cacgtaaatc ttctctggat gcagttcagg gtaaaaaaga
    18421 agatatagag cagattatag gtgaatattt tcaaactcaa ccaagattgg aattggaatt
    18481 aattagcggt aatacggtgg attctattgt catacctcat aaaactctgg aagacttaaa
    18541 aaaaatggat gagaatcttg ctcgttatat agaaattact gattcaaaac tcatatcata
    18601 atatatatta aaatgttata taattatgtt gtcttccgaa ttaccagtta cctgcttgtc
    18661 ccttagctct ttggataggt aaccgctaag aaataggtaa gttagagaaa ttgtaaaaat
    18721 cctgatttaa ggtgaagaag gcaattctat aatttactta tttataatga ctttatatct
    18781 attatatgac cgtttcaatg aataaattta aaggatgcca tctctctttt taaatttcct
    18841 ctgcttcttc tccataaatc tctttaaatc tgaccttatc tatatcttca ggatgacctt
    18901 caaagacttt tatcccttgt tttaagccta ccactctgtt tccatagcga cgggcaagag
    18961 taagaaaatg aagagaacaa ataacggaaa ctccttcccg attaatttct cccagatatt
    19021 gcataataga atcagcggta gcggggtcta aactggcaac tggttcatct gccaggatta
    19081 gttctggttc ttgcatcaaa gctcgtgcaa tagccactct ttgt
//
LOCUS       DAORZF010000031        18459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_2491_length_18459_cov_375.823354, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000031 DAORZF010000000
VERSION     DAORZF010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 18459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18459
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2491_length_18459_cov_375.823354"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            388..1362
                     /gene="phnE"
                     /locus_tag="PK015_04865"
     CDS             388..1362
                     /gene="phnE"
                     /locus_tag="PK015_04865"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HOL33831.1"
                     /translation="MNKLKSFLVEYLLWQYLWGCVAWMAYRLYSFSFPELSIFGIPLV
                     LGFIFGFQKNSLGAKVANPDKEIKGWFWKAEIWAIVIISLIVGWIIVEVKPIAFFTQA
                     GNAKNIVQGLFNPNFKQLVPMLAALMETVYLALLATIFAVPFAFILSFFAAKNLMYST
                     ILGRIVYYFIRTITTIVRSIEALVWAIIFCVWVGIGPFAGMLALWIHSIAALVKLYSE
                     QIESIDQGPVEAIVATGASTLQVWRYAVTPQILSPYLAFTIYRWDMNVRMATVVGFVG
                     GGGIGLALNQQQQMLAWRNVGLIIWLIALVVWVMDIFSGYLREKLVMT"
     gene            1373..2680
                     /locus_tag="PK015_04870"
     CDS             1373..2680
                     /locus_tag="PK015_04870"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HOL33832.1"
                     /translation="MINYNPVELILAKRNGTALNEDQIRNLVEGFLSGEVPDYQMSAL
                     LMAIYFQGLNKEEIFALTKTYIESGKTITFPAELKVADKHSTGGVGDKISLMLAPIAA
                     SLGLTIPMISGRGLGHTGGTLDKLESIPGFRTQFSIEEFRKLVLEHNCALAGQSKDLV
                     PADQKIYALRDVTATVESPGLITASILSKKIAEGAKHLIIDLKIGSGAFMPDREKAKE
                     LAELLISTGERFGLKVKVAFTNMNSPLGLAVGNGLEMIEAIEYLKGNPLKDTKILTSE
                     LTAQLLVSTNLAETAEQAIAMIEEAVKNGKALKKLEEIIVAQGGNPKVIDDYGLLSTT
                     KYKLPILSPASGYVSHIDCRAIGYALIHINAGRMKVTDSIDHSTGALLIPKIGDFLEK
                     DEPIGEVHCNDQIKGEEVVKLIQKAYTLSSEPIAEEPIIFDIV"
     gene            2969..4171
                     /locus_tag="PK015_04875"
     CDS             2969..4171
                     /locus_tag="PK015_04875"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOL33833.1"
                     /translation="MRILVLNCGSSSIKYQFIDMNNLLVLAEGIAERIGEDLSLFTYK
                     SEKYTLKKKEMVIENHEQGLQLIIDYLLDPVNGVIKDKSEIDAVGHRLVHAGEYYSDA
                     VVITDDVVRVMKECIPLAPLHNPANIKGIEAVKKNVPEVPQCGLFDTAYHQTMPPRAY
                     LYPLPLEYYEKYKIRRYGFHGTSHKYVSLEAARFLQKDISQLKIITCHLGNGASMTAI
                     ERGKSIDTSMGFTPLEGLMMGTRCGDIDPSIPIYMQQHLGLNVDEVNSILNKNSGMLG
                     LSQISNDMRFIEDEVLQKNNPKAMQALEVYCYRIKKYIGSYYAVMNGLDVLVFTGGVG
                     ENMPLAREMVCKDMEALGIKLDLTANAKFSSEIEVISVPDSRVTVLKVPTNEELMIAL
                     ETQRLLTQ"
     gene            4193..4456
                     /locus_tag="PK015_04880"
     CDS             4193..4456
                     /locus_tag="PK015_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HOL33834.1"
                     /translation="MRIDMLLNKLCLTKSRSIAKIACDKGLVSLNGKTAKASAEVKEG
                     DIIQFHLYGYEHQIRIDKIPQGNVAKKDATNYYTLLSRKEISN"
     gene            4630..5379
                     /locus_tag="PK015_04885"
     CDS             4630..5379
                     /locus_tag="PK015_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33835.1"
                     /translation="MGTGVYLDSRADIYVVPDEQSYELLTQGKGRIVEFSSAFYSSLD
                     HRIYIRSADQILSNYSGIIIHEYTHWLLDEILRRAPLWFHEGMATQQGNQLGLDRYYY
                     YIRERFWGNKMDLIKLAENYPQQPADWDLYYITSYYAVQYMKDNDPQAWKDFWEIVSN
                     NYRTGKKTIFSEAFHNAYHMDLWQFNQDFAAYSKRQAYVYIFTGLGTFILILMPFILI
                     YAHIKQRKKMKSLPDIDFPEETTEDEDENYY"
     gene            5396..6427
                     /locus_tag="PK015_04890"
     CDS             5396..6427
                     /locus_tag="PK015_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOL33836.1"
                     /translation="MFPVSFGSDNHSGVHPEVWKALLKADEGFAPAYGEDPLTLEVLN
                     KIESLFGGDCQACFVMTGTGANVVAIQNLVKSFNAIFCAQTAHINVDECGAVQKFTQA
                     RLQVIPTTNGKLTPELIGPYLLNNRDQHHSQGKVVSIAQSTEYGTLYTIEELKVLADY
                     THQQDMLLQIDGARLANAAAALDCSLKDITKEVGADIVSFGGTKNGLLFGEALISFRP
                     ELTSDIRFYRKQCTQLFSKMRYISAQFDAYLKDALWKKNASQANAMATLFSESLRNIP
                     EVEIVQPVQVNSLFVKMPKKSIEELREKYHFYIWDEAKNVVRLMCSFNTTEKHIVEFI
                     SDLKSSLVR"
     gene            complement(6439..7251)
                     /gene="pyrF"
                     /locus_tag="PK015_04895"
     CDS             complement(6439..7251)
                     /gene="pyrF"
                     /locus_tag="PK015_04895"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOL33837.1"
                     /translation="MERTLSKSLWHKYRARWQSSHSLLCIGLDSDITQLPECVLNERN
                     PIWEFNRRIIDSTAQYACAYKPNLAFYLRDGIRGLEALQLTINYIPEDIPVILDCKVG
                     DIGSTMEQYVSTFLEVMGFDAITINPLLGADVLKPILERENAFAFVLTLTSNPSAKDF
                     FIQEDMAVKIARWLTIFPPEKIGAVVGATQVNQLREMRELMPGHIFLIPGIGAQGGDL
                     DAVMHYTRDSDDYPNILINSSRGIIFQDRSPKFAQAAALAAEDLNSKIGVYT"
     gene            complement(7217..7753)
                     /gene="pyrR"
                     /locus_tag="PK015_04900"
     CDS             complement(7217..7753)
                     /gene="pyrR"
                     /locus_tag="PK015_04900"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOL33838.1"
                     /translation="MQVKSIIMDKDQMERTIHRMAHEIIEQNRGIENICFVGIRTRGV
                     PLARRLADYLRLIEGKDIPVGVLDITLYRDDLSMISHQPVIKGSELGFEIEGASIILV
                     DDVLYTGRTVRAAIDALLDFGRPQQIQLAVLIDRGHRELPIKADYVGKNVPTSKEEII
                     KVSFEEIDGEDSVKIVVA"
     gene            complement(7804..9813)
                     /locus_tag="PK015_04905"
     CDS             complement(7804..9813)
                     /locus_tag="PK015_04905"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HOL33839.1"
                     /translation="MQKHGILEYSEMRYEPDRPKAANPIKIQDLTFRDGHQSLFATRV
                     RTEDLLHVAELMDQVGFYSMEVWGGATFDVMHRYLGEDPWERIRVLKKHITKTPFSML
                     LRGQNLVGYRNYPDDIVETFVQRCCDNGIDIFRVFDALNDFRNFQTAVKIIKKNNKHF
                     QGAICFSLTEQRMGGEIYNIEYYVSKAKQLEDMGADTICIKDMAGLIAPYDAYDLIRA
                     LKENVKVPVHLHTHFTSGMGDLSLFKAIEAGVDIIDTCVGPYAYRTSHPAIEPLVISL
                     LGTNRDTGFDIKLLNKIGKEMEKDIPKYMQFADTTKFSIIDTDVIIHQTPGGMISNLI
                     NQLRQMDALDRLEDVFKEIPRVRKDLGQIPLVTPTSQIVGIQAVNNTLFDSYEGEYSH
                     ITEEVKDLCYGLYGKTPMPINPEVQKKALKGYPRGEKPITVRPGDILEPGLEKAKQEA
                     EGLAKDIDDVLIVALYNVTGKKFLRIKYGLEPMPEEMKPITLEEVKRQQELCEKAKAG
                     LLVEPPVKKQVPKPEGLRQFDVFVDDEYFLVEVSEKGVPHIVHSAKVASNPQVQPSQT
                     SNPQSSPQPVKNNPQPVPNQSSVQTASTAVEGTPVTAPMPGMLVRYEKKVGDKVKVGE
                     TLLVLEAMKMYNNIPSPVEGTVVSTPLNAGDSVGKGDVMIVVKPD"
     gene            complement(9823..10008)
                     /locus_tag="PK015_04910"
     CDS             complement(9823..10008)
                     /locus_tag="PK015_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33840.1"
                     /translation="MTDKKKEVAAIAGVLALITAEEAEAPKLPQARASLLWQAYGRQQ
                     TMLYRDLTQRRLIKRHK"
     gene            complement(10029..11582)
                     /locus_tag="PK015_04915"
     CDS             complement(10029..11582)
                     /locus_tag="PK015_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOL33841.1"
                     /translation="MPSTKDAIQKLLEKEQKVLQLGGPQAVEKQKQKGKLTARERIDL
                     LFDPGTFRELDMFVQHRCDNFGMDKVEIASDGVITGHGLVNGRPVFAFSQDFTSRGGS
                     LGEMHAAKICKIMDMALKNGLPLIGFQDSGGARIQEGVDALKGYGDIFFRNSRASGVI
                     PQITAIMGPCAGGAVYSPAMTDYVFMVKNTSYMFITGPEVIRAVTGEETTQEELGGAM
                     AHNTKSGNAHFACENDADAIEQIKVLLSYLPSNNMEEPPRIECNDDPWRLCPNLDTII
                     PDNPKESYDMHDVIKTIVDDGIFFEPHKYYAQNIIVGFGRLNGRVIGVVASQPTVLAG
                     CLDIDASDKATRFIRFCDAFNIPLVTFVDVPGYLPGTYQEHNGIIRHGAKLLWCYSEA
                     TVPKLTVITRKDYGGSYIAMSSRHLGADMVFAWPTAEIAVMGAEGAANIIFRKEIDAA
                     EDKAAKRAEMIKIYEEQFNNPYVAASRGYIDAIILPSETRKRLIDALEICSTKSESLP
                     PKKHGNIPV"
     gene            11793..13349
                     /locus_tag="PK015_04920"
     CDS             11793..13349
                     /locus_tag="PK015_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HOL33842.1"
                     /translation="MLKLELDPDMQNRARKAAASIAGSFQWLFDDYSSVTIERTVLRL
                     LGIDGATEDGTPYPNIVVDHLQQRNALLRGAAIPLANAIIYTGMSAQQIAEEIAQGNL
                     DLTKTPVASPDKIFQVLDELSDKMLKHIAKQRETREQLIQTYGPRRIPAIYVIVATGN
                     IYEDVIQAKAAAYEGADIIAVIRSTAQSLLDYVPYGATTEGFGGTYATQENFRIMRAA
                     LDEVSVSEKRYIGLTNYASGLCMPEIAAMGAMERLDHMLNDAMYGILFRDINPKRTLL
                     DQYFSRMINAYAGIEIQTGEDNYLTTSDAIEKAYTVTASQLLNEQFALLSGVKPEKMG
                     LGHAFEIDPDLEDSFTYELAHALLTRELFPGYPVKYMPPTKFATGNIFKTYLMDAMFN
                     FAGKLTHQGVQLLGMMTEAIHTPHLADRSLAIENALYIFNAIKSLDENITLVPDSFIN
                     RRANQVLKEATEFLERVEKEGLFKAMERGEFADIKRTENGGKGLNGVFRKDVEYFNPF
                     LEKMKKELGL"
     gene            13346..14092
                     /locus_tag="PK015_04925"
     CDS             13346..14092
                     /locus_tag="PK015_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HOL33843.1"
                     /translation="MKHNIIRPYGDKSGDGKVQLSFTLPVPPDDWGKEAAKQLLKQMG
                     FEDIIICGIRDLHEGFTHFIAYAVTPCSVDSSKIKVKKVDTPVMEMEEVDEFIKENIG
                     RKITVVGACIESDAHTVGIDAIMNMKGYNHRYGLERYQWFNAINLGAQVPCEELLRRA
                     REENADAVLVSQVVTQNNIHIHNLTRLIELAETEKLRDKMLFICGGPKISHELAIELG
                     YDAGFGPGTYANEVASYIAVTLAKKLAQKS"
     gene            14174..15460
                     /gene="rseP"
                     /locus_tag="PK015_04930"
     CDS             14174..15460
                     /gene="rseP"
                     /locus_tag="PK015_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOL33844.1"
                     /translation="MTLLLTILAFGLMIFVHELGHFLMAKAWGVGIEQFSIGFGKPIA
                     QFTSHNVQWRIGWIPLGGYVKMKGENPDEQNIDDESALSRKPWWQKAIIGFSGPFANI
                     VLTLILFIWSFMMPVYIEDQLPVVYKAEGKWAQVFAPGDSIISVNGKNIRGYSEFLQE
                     LLSNPENTVLLSRNGNKQTVLISSSEVDSLAKSLYPYADTRIGEVVPKTPAYHSKLEP
                     GDRIVAIDSLPVSNWYEMREIIVNSEKDEVLLTIERNGQRFNKVLPLESSINTGGNKA
                     IGIIQYQPVKYERRYKPLEAIQLGAVNTVNFIVLNYQALGKLVQKPQDLTKSVGGPVM
                     IASMSQSFGKKGFGSLLFFFGSISLMLAIMNLLPIPVLDGGLILFAIIEGIIRKPVPQ
                     QVQSILQTIGFFILIFLMVLAFYSDIASEVLRFMNR"
     gene            15467..16576
                     /gene="tgt"
                     /locus_tag="PK015_04935"
     CDS             15467..16576
                     /gene="tgt"
                     /locus_tag="PK015_04935"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOL33845.1"
                     /translation="MFKFQLEKVSNKARAATITTSHGKIKTPVFMPVGTVGTVKAMSP
                     EELEKCHTQIILGNTYHLYMRPGYELIQQAGGLHKFIAWKHPILTDSGGFQVMSLAGL
                     RKITNEGVKFQSHLDGSTHFFTPESVIAIQEALGADIIMSFDECPPFPSTREYVEKSL
                     QRTLKWAERGKAAFHNYKNQALFGIVQGGIYEDLREKAAKTLMEMDFPGYAIGGLAVG
                     EDKADMFRITNFLNDILPADKPRYLMGVGTPSDLLLNIANGVDMFDCVMPTRNARKGS
                     IFTRQGKMIIKAARYKDDFQPIDKQCGCYTCKHFSRAYIRHLITMNEILGMRLTTIHS
                     LYFYNELMEMARQAIMEDRYEEFLAEMLPVLDRVI"
     gene            complement(16998..17360)
                     /locus_tag="PK015_04940"
     CDS             complement(16998..17360)
                     /locus_tag="PK015_04940"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOL33846.1"
                     /translation="MNCIFCLISPDKYLAENEYFFAIRDIHPITKGHSLIISKRHTPD
                     FFTLQPEEMTALLEISRIMRNLLDEELHPEAYNLLMNCGSAAHQSIFHFHLHFIPRYH
                     KDGLSLRAINEKLRSIAF"
     gene            17646..17822
                     /locus_tag="PK015_04945"
     CDS             17646..17822
                     /locus_tag="PK015_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HOL33847.1"
                     /translation="MPDKTLICKDCQKEFVFTEGEQEFYQEKGLVNEPQRCPECRKAK
                     KARYNRNRYQHNAR"
     gene            complement(17939..18121)
                     /locus_tag="PK015_04950"
     CDS             complement(17939..18121)
                     /locus_tag="PK015_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33848.1"
                     /translation="MVNREAYVDGQSVGIDPEYDANTIPLRVGTHHLEIRSKNRILLA
                     DDIVIEPNKVTQVTVP"
BASE COUNT         5737 a         3439 c         3518 g         5765 t
ORIGIN      
        1 acaagctcct tcggagcttc ataaacgggg acgtttatga ccccgtgtaa gagaacgttt
       61 atgaccccgc tatggggtgc gatatgtccc cttatttatc actcacttat aactcaaaaa
      121 attactgcac atttacaata cgccatttac tgaagtattt atatcacatc ttaatcaagc
      181 gttaatcaag tcttaatata tttacgcttg attaacgagt gattaacact tgatagcagg
      241 aaagagtcag gtagaaccca taatttagac cggatttaca attaaaatag aaaaaataat
      301 tcccataaca tttcttgcaa gaaccttcaa gactaagtct gataaaaaat tcttgacgaa
      361 ctaagcattt caaatatgat aacttatatg aataaattga aatccttttt agttgaatat
      421 ttgctctggc aatatttgtg gggttgtgtt gcctggatgg cttatcgtct ttattctttc
      481 tcatttcctg aactttcaat cttcgggata cctcttgttc ttggcttcat atttggattt
      541 cagaaaaata gtttaggcgc taaagttgcc aatcctgata aagaaattaa gggttggttc
      601 tggaaagcag aaatctgggc aattgtaatt atcagtctta tagtaggctg gattattgtg
      661 gaagtaaagc cgattgcttt tttcactcag gcaggaaatg cgaaaaatat agtgcagggt
      721 cttttcaatc ccaattttaa acagttagtt ccaatgctgg cagctttgat ggaaacggta
      781 tatcttgctc ttttagccac tatttttgcg gtaccttttg cttttattct cagttttttt
      841 gccgcaaaaa atctaatgta ttcaactatt ttaggaagaa tcgtttacta ttttatacgc
      901 actatcacta cgattgttcg ttccatagaa gccttggtct gggcgattat tttctgtgtt
      961 tgggtgggca taggtccttt tgctggaatg cttgctttgt ggatacattc aatcgctgct
     1021 ttagtgaaac tttattcaga acagatagaa agcatagatc aaggaccagt ggaagctatt
     1081 gttgccacag gagcatccac ccttcaggtt tggcgttatg ctgtgacccc tcaaatccta
     1141 tcaccctatc tggcttttac tatctataga tgggatatga atgtgcgaat ggcaacggtc
     1201 gtaggatttg taggtggtgg aggaattgga ttggcattaa atcaacaaca gcaaatgctt
     1261 gcctggagaa atgtaggact cattatctgg ttaattgctc tggtagtttg ggttatggat
     1321 atatttagtg gctatctaag agaaaaattg gtaatgactt aaggagaaaa agatgataaa
     1381 ttataatcca gtagaactta ttctggctaa aagaaatgga actgcattaa atgaagacca
     1441 gataagaaat ctggtagagg gttttttaag tggagaagtt cctgattatc agatgagtgc
     1501 acttcttatg gctatctatt ttcaaggttt aaataaagaa gagatttttg ctctaactaa
     1561 aacatatatt gaaagtggaa aaacaataac ttttccagca gaactcaaag tagccgataa
     1621 acattctact ggaggggtag gagataaaat cagtctaatg cttgccccta ttgcagcttc
     1681 cctcggattg accataccta tgatatcagg aagaggatta ggtcacactg gtggcacttt
     1741 agataaactt gaatctatcc ctggttttcg tacacaattt tctatagaag aatttcgcaa
     1801 gctggtttta gaacataact gcgcgcttgc cggtcaatct aaagacctgg tccctgctga
     1861 ccagaagatt tatgccttgc gagatgtaac tgctacagtt gaaagtccag gtttgataac
     1921 tgccagtata ttgagtaaaa aaatagcaga aggagcaaaa catctgatta ttgacctcaa
     1981 gataggaagc ggtgctttta tgccagatag agaaaaagca aaagaattag ctgaattgct
     2041 catctctact ggagaacgat ttggtttaaa agtgaaagtt gcgttcacca atatgaactc
     2101 tcctctcggt ttagctgtag gaaatggtct ggaaatgatt gaagctatag aatatttaaa
     2161 aggaaatcct ttaaaagata caaagatatt aacttccgaa ctcactgctc aactgcttgt
     2221 aagcaccaat cttgctgaaa ctgcagaaca agctatagct atgattgaag aagctgttaa
     2281 aaatgggaag gcattaaaaa aactggaaga aatcattgtt gctcagggtg gaaatcctaa
     2341 agtaattgat gactacggtc ttttgagcac gactaaatat aaacttccca tcctatctcc
     2401 tgcttctggc tatgtttccc atatagattg tcgcgcaata ggttatgcac tgattcatat
     2461 caatgcagga agaatgaaag tgaccgattc catagaccat agcacaggcg ctctcttaat
     2521 cccaaagatt ggagatttcc tggaaaaaga tgaacctatc ggagaagtgc attgtaatga
     2581 ccaaattaaa ggtgaggaag tggtaaaact tatccagaaa gcttatactt taagttcaga
     2641 acctatagca gaagagccca ttatctttga tattgtataa ttaaaaaagt atcagaaaaa
     2701 atagaatgtt gaatttttaa ggaacctatt cctaatctta agctggatca aaagtaccct
     2761 taaaatgatg tagttgtttg caaataggaa tgttacattc ttgcgaatta agatggtaat
     2821 agaaagtaaa aaagattatt ctttcctgac gagagagata atttccccca tgaagaaaga
     2881 ctatttccgt gttcgcaaag taaatcagtt gacaataagg cattattatc tttttttgaa
     2941 ataaaaaaat attataaaga aggacaatat gagaatatta gttctcaatt gtggcagttc
     3001 ctccataaaa tatcaattta ttgatatgaa caatctatta gttctggcag aaggaattgc
     3061 tgaacgaatt ggtgaagacc tatcactttt cacttataaa agcgagaagt atactttaaa
     3121 aaagaaagag atggtaattg aaaatcatga acagggttta caactaataa tagattattt
     3181 attagaccca gttaatggag tgattaagga taaaagtgag attgatgctg taggtcatag
     3241 attagtgcac gctggtgaat attattctga tgctgtagtg attaccgatg atgtagtaag
     3301 agtgatgaaa gaatgtattc ccctggcacc cttacataat cctgccaata taaagggaat
     3361 agaggcagtt aaaaagaatg tgcctgaggt tcctcaatgt ggactttttg atacggcata
     3421 tcatcagact atgcctccaa gagcttatct atatcccctt cctttggaat actatgaaaa
     3481 atataagatt agaagatatg gttttcatgg aacttcgcat aaatatgtaa gtttagaagc
     3541 tgccagattt ttgcaaaagg acatttctca attaaagatt atcacctgtc atctgggaaa
     3601 tggtgcttca atgacagcta tagaaagagg aaaatctatt gatacttcaa tgggttttac
     3661 tcctctggaa gggttaatga tgggaactcg ttgtggagat attgatcctt ctattcccat
     3721 atatatgcaa caacatctgg gcttaaatgt agatgaagtg aatagcattt taaacaaaaa
     3781 cagtggaatg ctgggactct ctcagatttc taatgatatg cgatttatag aagatgaagt
     3841 ccttcaaaaa aataatccta aggctatgca agctctggaa gtttattgct atcgcattaa
     3901 gaaatatatc ggctcttatt atgcggttat gaatggtctg gatgttttag tatttacagg
     3961 tggagtagga gaaaatatgc ctttagccag agaaatggtc tgtaaagata tggaagcttt
     4021 aggcataaaa cttgatttaa cggctaatgc aaaatttagc agtgaaatag aagtgataag
     4081 tgttcccgat tcccgggtaa ctgtattaaa agttcccacc aatgaagaat tgatgatagc
     4141 cctggagaca caaagactac taactcaata aatcatcaaa tctgggggat ttatgcgcat
     4201 agatatgcta ctgaataagc tttgccttac taaaagtcgt agcatcgcta aaatagcctg
     4261 tgataaaggt ttagttagtc tcaatggcaa aaccgctaaa gcctcagctg aggttaaaga
     4321 aggagatata attcaatttc atctctatgg atatgaacac caaatccgga ttgataagat
     4381 tcctcagggg aatgtggcta aaaaagatgc cacgaattat tatactcttt tgagtcgcaa
     4441 ggagatttca aactgaaatc ggcaatctgg aaatgcttag ttctatttct atttatattt
     4501 agctttctta atgcaaacta tcagatagct gttgtaaccg ataactttgt cattcatacc
     4561 atagacggta aatttcctgt aacgcaaagc tttataaatg aattaaacca gagaatagat
     4621 cagctacaga tgggcacggg agtttatctg gattctcgcg ctgatatcta tgtggtgcct
     4681 gatgaacaat cttatgaact attaactcag ggtaagggaa gaattgtaga attttccagt
     4741 gctttttact cttctttgga tcatcgtatc tatatccgta gtgcagatca gatattatct
     4801 aactatagtg gaataataat acatgaatat acgcactggt tattagatga gattttaaga
     4861 agagctccac tttggtttca tgaaggaatg gctactcaac agggtaatca acttggactg
     4921 gatagatact attattatat aagagagcgg ttctggggca ataaaatgga cctgataaag
     4981 ttagcagaaa actatcctca acaacctgca gattgggatt tatattatat aacttcatat
     5041 tatgcagtgc aatatatgaa agataacgac cctcaagcct ggaaagattt ctgggaaatc
     5101 gtgtctaaca attatcggac cggaaagaaa acaattttct cggaggcatt tcataatgcc
     5161 tatcatatgg acctctggca attcaatcaa gattttgctg cttatagtaa aagacaggct
     5221 tatgtttata tttttactgg tttggggact tttattctga ttttaatgcc ctttatcctc
     5281 atctatgctc atattaagca aagaaaaaag atgaaatcgc tacccgatat agattttcct
     5341 gaggaaacca cagaagatga ggatgagaat tattattaag ttttaaggag atactatgtt
     5401 tcctgtaagt tttggaagtg ataatcattc aggagtgcat cctgaggtct ggaaagctct
     5461 tctcaaagca gatgaaggtt ttgctccagc ttatggagaa gaccctttaa cccttgaagt
     5521 tttaaataag attgaatcac tctttggagg agactgccaa gcctgctttg ttatgactgg
     5581 aactggtgct aatgtggtag ctatacagaa cctggttaaa tcttttaatg ctatcttttg
     5641 tgctcaaact gcgcatatca atgtggatga atgtggtgca gtacaaaaat ttacccaagc
     5701 aagattacag gttattccga ccactaatgg taaattgact ccagaattaa tcggtcccta
     5761 ccttctaaat aatagagacc agcatcattc tcaaggcaag gttgtttcca ttgctcaaag
     5821 cactgaatat gggactttat ataccattga agagcttaag gtcctggcag attacactca
     5881 tcagcaagat atgctcttac agatagatgg tgcaagactt gccaatgcag ctgcagcttt
     5941 ggactgttca ttaaaagata ttactaaaga agtcggtgca gacattgtta gctttggagg
     6001 aacaaaaaat ggacttcttt ttggagaagc attaattagt tttagaccag aacttacctc
     6061 cgatatcagg ttttatcgta aacaatgtac acaactattc agtaagatgc gatatatctc
     6121 tgcgcaattt gatgcttatc tgaaagatgc attatggaaa aagaatgcca gccaagccaa
     6181 tgctatggca actcttttta gtgaaagttt aaggaacatt ccagaagttg aaatagtaca
     6241 acctgtgcag gtaaattcat tatttgttaa aatgccgaaa aaatccattg aagaactgag
     6301 ggagaaatat catttctata tctgggatga agcaaaaaat gtggtgcgct tgatgtgcag
     6361 ctttaatacc acagaaaaac atattgtgga atttatctcg gaccttaaaa gtagtttagt
     6421 tagatagctc tgaactgatt aggtgtatac tccaatcttg ctatttaaat cctcagcagc
     6481 taaagcagca gcttgagcaa atttaggaga cctatcctga aagatgatgc ctctggaaga
     6541 attgatcaag atattgggat aatcatctga atctctggta taatgcataa ctgcatccag
     6601 gtctcctcct tgagcaccga ttccaggaat caaaaagata tgtccaggca taagctctcg
     6661 catttctcgt aattgattca cctgagttgc accaactact gctccaattt tctctggtgg
     6721 aaaaatggtc agccatctgg ctatctttac agccatatct tcttgaataa agaaatcctt
     6781 agctgaagga ttggaagtga gagtgagcac aaaagcaaaa gcattctctc tttccaagat
     6841 aggcttaagc acatcagcac ctaaaagtgg attgattgtt atggcatcaa aacccatcac
     6901 ttccagaaaa gtggaaacat attgttccat agtagaacca atatccccca ctttgcaatc
     6961 cagaataaca ggaatatctt caggaatata attgatagtc agttgtaaag cttctaatcc
     7021 acggataccg tctcttaaat agaaagccag attaggttta taggcacaag catattgagc
     7081 tgtagaatca attattcttc ggttgaattc ccagataggg ttcctttcat ttagaacgca
     7141 ttctggaagt tgagttatat ccgaatctaa tccaatacaa agtaaggaat gagagctttg
     7201 ccagcgagct ctatatttat gccacaacga ttttgacaga gtcctctcca tcaatctctt
     7261 caaaagaaac tttgattatc tcttccttag aagtgggaac atttttcccc acataatctg
     7321 ctttgatagg aagttcacga tgaccacggt ctatcaggac tgccaattga atctgttgag
     7381 gtctgccaaa atccaacaag gcatcaatag cagcacgaac agtcctacct gtataaagca
     7441 catcatccac cagaataatg gaagcaccct caatctcaaa acccagttca gaacctttaa
     7501 tcacaggttg atgagatatc atagagaggt catcacgata cagagttata tccagaactc
     7561 cgacaggaat atcctttcct tcaattaggc gaagataatc tgccaatctt cgggctaagg
     7621 gcactcctct ggttcggata cctacaaaac agatattttc aatacctcta ttctgttcaa
     7681 ttatctcatg cgccattcta tgaatggtgc gttccatctg gtccttgtcc attatgatac
     7741 ttttaacttg catctctcac tcctgagaat ggttctctaa aatgaagcct ttttggcgac
     7801 ttattaatct ggtttcacta ctatcataac atcacccttc ccaacactat ctccagcatt
     7861 taacggagtg gaaacaactg ttccttctac gggactgggt atattattat acatcttcat
     7921 cgcttccagc accagtaaag tttcccctac tttcactttg tctcccactt ttttctcata
     7981 acgcactaac atcccaggca ttggagcagt gactggagtt ccttccaccg cagttgacgc
     8041 agtttgaact gaagactgat taggaacagg ttgaggattg ttttttacgg gttgaggaga
     8101 tgactgtgga tttgaggttt gactgggttg cacctgagga ttggaagcta ctttagcact
     8161 atgaacaata tgggggacac ccttttctga cacttctact aagaaatact catcatctac
     8221 aaacacatca aactgtctca aaccttcagg ctttggaact tgctttttaa ctggtggctc
     8281 taccagaaga cctgctttag ccttttcaca tagctcctgc tgtcttttaa cctcttccag
     8341 agtaataggt ttcatctctt ctggcatggg ctctaaacca tattttatcc tcaggaattt
     8401 cttccctgtg acattatata gagctacaat taggacatca tcaatatcct tagctaaacc
     8461 ttctgcttcc tgttttgctt tttccagtcc aggttcaaga atgtccccag gacgaactgt
     8521 gatgggtttt tctcctcggg gataaccctt taatgctttt ttctgcacct ctggattgat
     8581 gggcattgga gtcttaccat aaagtccata gcagaggtct ttcacttctt ctgtgatatg
     8641 ggaatattcc ccttcatagc tatcaaagag agtgttatta actgcctgaa tacctacaat
     8701 ttgactggta ggagtaacta aaggaatctg tcccagatct tttcgtactc tcggtatttc
     8761 tttgaaaaca tcttccagac ggtctaaagc atccatctga cgaagctgat tgataaggtt
     8821 ggaaatcatc ccacctggag tttgatggat aatgacatca gtatcaataa tgctaaattt
     8881 ggttgtatcc gcaaattgca tatattttgg aatgtctttc tccatttcct ttcctatttt
     8941 attaagcagt ttaatatcaa aaccagtgtc acgatttgtc cctaaaagag aaattaccag
     9001 aggttcaatc gctggatggg aagttctata ggcatagggt cccacacagg tatctatgat
     9061 atccactcct gcttcaatag ctttaaacaa ggataaatct cccattccgc tggtaaaatg
     9121 agtgtgtaga tgtactggga ctttaacatt ttctttcaaa gctctaatca agtcataagc
     9181 atcataagga gcaattaatc ctgccatatc cttgatacag atagtatcag ctcccatatc
     9241 ttccaattgt ttagctttgg aaacataata ttctatatta tatatttctc cacccattct
     9301 ctgttcagtc aggctgaaac agatagcccc ctgaaagtgt ttattattct tttttataat
     9361 cttgacggca gtctgaaaat tacggaaatc attaagtgca tcaaacaccc gaaagatatc
     9421 aattccgtta tcacaacatc tttgcacaaa ggtttctaca atatcatctg gataattacg
     9481 atatcccacc agattttgac ctcttaagag catagaaaat ggtgttttag tgatatgttt
     9541 tttcaggacc cggatgcgtt cccaggggtc ttctcctaaa taacgatgca taacatcaaa
     9601 ggttgctcca ccccagactt ccatagaata gaagcctacc tggtccatta gttctgctac
     9661 atgtaataaa tcttctgtcc taacgcgtgt agcaaagaga gattgatgtc catcacggaa
     9721 ggtcagatcc tgaatcttga tgggatttgc cgctttaggg cggtccggtt catagcgcat
     9781 ctcgctatat tccaagatgc catgtttctg catttattct ccttatttat gtctttttat
     9841 taatcttctt tgggttaaat cacgataaag cattgtctgt tgacgaccat aagcttgcca
     9901 gagcaaggaa gctcttgctt gaggaagctt tggtgcttct gcttcttctg ctgtaattaa
     9961 tgccagaact cctgctatgg cagcaacttc ttttttctta tctgtcattt atcatatcct
    10021 gaaatgtatt aaactggtat atttccgtgc tttttaggag gcaagctttc acttttggta
    10081 gagcaaatct ccagcgcatc aattaatctc tttctggttt cggaaggaag gataattgca
    10141 tctatatagc cacgagaagc tgccacataa ggattattga attgttcttc atagatttta
    10201 atcatttccg ctcttttagc tgccttatct tctgctgcat caatctcttt tctaaagata
    10261 atattggcag caccttcagc acccataacc gcaatttctg ctgtaggcca ggcaaaaacc
    10321 atatctgctc ctaagtgtct actactcatt gcaatatagc taccaccata gtctttgcgt
    10381 gtgattacgg tcagtttagg aacggtggct tctgaataac accaaagcag ctttgcacca
    10441 tgccttatta ttccattatg ttcctgataa gttccaggaa gataacctgg cacatccaca
    10501 aaggtcacca gaggaatatt gaaagcatcg cagaaacgga taaaacgagt ggctttatcc
    10561 gaagcatcaa tatccagaca acctgccaag acagtaggtt gacttgctac cactccaata
    10621 actcttccat ttaatctacc aaaacctact attatattct gagcatagta tttatgtggt
    10681 tcaaagaaaa taccgtcatc cactatggtt ttaatcacat catgcatatc atagctttct
    10741 ttaggattat ctgggatgat agtatccaga ttgggacaaa gacgccaggg atcatcatta
    10801 cactcaatcc tgggaggttc ttccatatta ttggaaggca gatagcttaa cagaacttta
    10861 atttgttcta tggcatcagc atcattttca caggcaaaat gagcattccc actcttagta
    10921 ttatgagcca tcgcaccacc aagttcttcc tgagttgttt cttctcccgt aactgctctt
    10981 attacttcag gtccggtgat aaacatataa ctggtgtttt taaccataaa tacataatcc
    11041 gtcattgcag gagaatagac tgctccacct gcacagggac ccataatagc agtgatttga
    11101 ggaataacac cactggcacg tgaattacgg aagaagatat caccataacc tttaagagca
    11161 tctactcctt cctgaattcg tgctccacca ctatcttgaa atccaatgag tggtaatcca
    11221 ttttttaaag ccatatccat tatcttacag attttagcag catgcatttc tcccagagaa
    11281 ccaccacgag aagtgaaatc ttgtgaaaaa gcaaagactg gtcttccatt aaccaaaccg
    11341 tgtcctgtaa tcacaccatc cgaagcaatt tccactttat ccatcccaaa attatcacag
    11401 cgatgttgca caaacatatc cagttcccga aaagtgcccg gatcaaatag aaggtctatt
    11461 ctttcacgag ccgtcagttt ccctttttgt ttttgctttt ctacagcttg aggtcctccc
    11521 aattgaagca ccttttgctc tttttccagt aatttctgga tggcgtcttt agttgaaggc
    11581 atcttattgt ccccttttat ctaggttcgt aattcaaggc atataccgaa ttcttcattt
    11641 atatatatgc ttattttaca ttttaaaata gctaagtttt tcgtcaagta aaagcaaaat
    11701 gaattcttta tcctttccca tttcccttga cggaaataaa tgatcaaaaa atggtgtaat
    11761 taatatgtaa ttagacaatt ggagattttt taatgcttaa actggaactt gaccctgata
    11821 tgcaaaaccg tgccagaaaa gcagctgcct ctattgctgg ctcttttcaa tggctctttg
    11881 atgactactc ttccgtcact attgaacgaa ctgttcttag attgctcggt attgatggtg
    11941 ctactgaaga tggaaccccc tatcctaata tagtagttga tcacttacag cagaggaatg
    12001 ctttgcttag aggtgctgct attcctctgg ctaatgctat tatttacact ggaatgtcag
    12061 ctcaacagat tgcagaagag atagctcaag gcaatcttga tttaacgaag acaccagttg
    12121 cttctcctga taaaatcttt caggtgctgg atgaactttc agataaaatg ctgaaacata
    12181 tagcaaaaca gagagagacc agggaacaac ttattcagac ctacggaccc agaagaattc
    12241 cagctatcta tgttattgtt gccacaggaa atatttatga agatgtgatt caagctaaag
    12301 ccgctgctta tgaaggagct gatatcattg cggttattcg ttctactgct caatcgcttc
    12361 tggattatgt accttatggt gcaactacgg aaggtttcgg aggcacttat gccactcagg
    12421 aaaatttccg gattatgcga gctgcattgg atgaagtttc cgtttcagaa aaaagatata
    12481 taggtttaac taactatgca tcaggacttt gtatgccaga aattgccgct atgggggcaa
    12541 tggaacgtct ggaccatatg ttaaacgatg cgatgtatgg aattctgttc cgagacatca
    12601 atcccaaaag aactttatta gaccaatatt tcagcagaat gattaatgct tacgcaggaa
    12661 tagaaattca aaccggagag gataattatt taaccacttc cgatgccatt gaaaaagctt
    12721 acactgttac cgcttctcaa ttgctaaatg aacagtttgc cctcttgagt ggtgtgaaac
    12781 ctgagaaaat gggattagga catgcttttg agattgatcc tgatttggaa gatagcttca
    12841 cttatgaact ggcacatgct ctgttaaccc gtgaactctt tcccggttat cccgtgaaat
    12901 atatgcctcc aacgaagttt gctactggca atatttttaa aacctatctt atggatgcta
    12961 tgtttaactt tgccggaaaa ttaacccatc aaggggtgca attactggga atgatgaccg
    13021 aagcaattca tactcctcat ttagcagacc gttctttagc tattgaaaat gcactttata
    13081 tttttaatgc cataaaaagt ctggatgaga acattactct ggtccccgat agtttcatca
    13141 atagaagagc caatcaagta ttgaaagaag ctactgaatt cctggaaaga gtggaaaaag
    13201 aaggtctttt caaagctatg gaaagaggtg aatttgccga tataaaacgt actgaaaacg
    13261 gtgggaaagg cttaaatggc gtgttccgta aagatgtgga atattttaat ccttttctgg
    13321 aaaagatgaa aaaggagctg ggattatgaa acacaacatc atccgtcctt atggtgataa
    13381 atcaggcgat ggaaaagttc agctttcctt cactttacca gttccaccag atgattgggg
    13441 aaaagaagca gccaaacaat tattaaagca gatgggcttt gaagatatca taatctgtgg
    13501 catacgagac cttcatgaag gtttcaccca ttttattgcc tatgctgtaa ctccttgttc
    13561 cgttgatagt tctaaaatta aagttaaaaa agtggatacc ccagtgatgg aaatggaaga
    13621 ggtggatgaa tttattaaag agaatattgg acgcaaaata accgtcgtag gtgcttgcat
    13681 agaaagtgat gctcacaccg ttggcattga tgccattatg aatatgaaag gctataatca
    13741 tcgttatggt ctggaacgct atcaatggtt taatgctatt aatcttggtg ctcaagtgcc
    13801 atgtgaagaa ttattgcgtc gggcacgaga agaaaatgct gatgcagtgc tggtttctca
    13861 ggtcgtaaca caaaataata tccatattca taatctcacc cgtcttattg aattagcgga
    13921 aactgaaaag ttacgagata agatgctctt tatttgtggt ggaccaaaaa tctctcatga
    13981 attggcaata gaactcggtt atgatgcagg ttttgggcct ggaacttatg ctaatgaagt
    14041 tgctagctac attgctgtta ccttagctaa aaaattagcc cagaaatctt aggtatatcc
    14101 tctgctgatt cttataacta tcaatctttc agtatgtttt atcagcttca aagtcagttt
    14161 ttaggataac cctatgacct tacttttaac tatcctggct tttggcttaa tgatttttgt
    14221 acatgaacta ggtcattttc ttatggctaa agcatgggga gtaggtattg agcagttctc
    14281 tattggtttt ggcaaaccta ttgctcaatt taccagtcat aatgtgcaat ggcgtatagg
    14341 atggattccc ctgggtggct atgttaaaat gaaaggtgag aatccagatg agcagaatat
    14401 agacgatgaa agtgctttat ctcgcaaacc ctggtggcaa aaagctatca ttggatttag
    14461 tggacctttt gccaatatcg ttttaaccct tatacttttt atctggtctt ttatgatgcc
    14521 tgtctatatt gaagaccagc tgccagttgt atataaagca gaaggtaaat gggctcaagt
    14581 ttttgctcct ggagatagca ttataagcgt aaatggaaaa aatatccgag gctatagtga
    14641 attccttcag gaattacttt caaatcctga aaacaccgtc ttacttagcc gtaatggcaa
    14701 caaacaaact gtgctaatct cctcttctga agtggattct ctggctaaaa gtctctatcc
    14761 ttatgctgat actcgcattg gagaagtagt gcccaaaact cctgcttatc attctaaatt
    14821 ggaacctgga gaccgtattg tagcaattga ttctctacct gtttctaatt ggtacgaaat
    14881 gcgtgaaatt atcgtcaatt ctgaaaaaga cgaggtgctt ttgactattg aacgcaatgg
    14941 acagaggttc aacaaagtgc tacctttgga aagcagtatt aatactggtg gaaacaaagc
    15001 tattggcatt atacaatacc aacctgtaaa atatgagcgt cgttataagc ctctggaagc
    15061 aattcaatta ggggcagtca atacagttaa tttcatcgtt ctgaattatc aggcactggg
    15121 aaaattggtt cagaaacctc aagatttaac caaatcagtg ggtggaccgg ttatgattgc
    15181 cagtatgagc caatcttttg ggaaaaaagg atttggcagt cttctctttt tcttcggcag
    15241 tatcagtctt atgcttgcta ttatgaatct gcttcctatt cccgttctgg atggtggact
    15301 gattcttttt gctatcattg aaggtatcat ccgaaaacct gttcctcaac aagttcaatc
    15361 tatacttcaa actataggat tctttattct catctttctg atggttttag ccttctattc
    15421 ggatattgca agtgaagttt tacgatttat gaataggtag gacaagttgt tcaaatttca
    15481 attagaaaaa gtttctaaca aagccagagc agccacaatt accaccagtc acggtaagat
    15541 taaaacccca gtttttatgc ctgttgggac agttggaacc gtgaaagcaa tgagtccaga
    15601 ggaactggaa aagtgccaca ctcaaattat ccttggaaac acttaccatc tttatatgag
    15661 accaggctat gaacttatcc agcaagcagg tggtctacat aaatttattg cctggaaaca
    15721 tcctattctg accgatagtg gtggctttca agtgatgagt ctggcaggac ttagaaaaat
    15781 cactaatgaa ggggtgaaat ttcaatcgca cttagatggt agcacgcatt tttttacccc
    15841 tgaaagtgtt atcgctattc aagaggcact tggtgcagat ataattatga gttttgatga
    15901 atgtcctccc tttccttcaa ctcgtgaata tgttgaaaag tccttgcaac gcactttaaa
    15961 atgggcagaa cgaggaaagg cagcttttca taattataaa aatcaggctc tctttggcat
    16021 agtacaagga ggcatctatg aggatttacg agaaaaagca gctaagactt tgatggagat
    16081 ggattttcct ggctatgcta ttggaggttt agctgtcggt gaagataaag ctgatatgtt
    16141 ccgtataacc aatttcttga atgatatact tcccgcagat aagcctcgtt atctgatggg
    16201 tgtgggtact ccttccgatt tattattaaa tattgctaac ggagtagata tgtttgactg
    16261 cgtgatgcct acccgaaatg cccggaaagg aagtattttc actcgccagg gaaaaatgat
    16321 tatcaaagct gctcgctata aagatgattt tcaacctatt gataagcaat gcggatgtta
    16381 cacttgtaaa catttcagtc gtgcctatat tcgtcattta attacgatga atgaaatcct
    16441 gggaatgcgt ttaaccacca tccacagtct atatttctac aatgaattaa tggaaatggc
    16501 tcgtcaggca ataatggaag accgttatga ggaattctta gcagagatgc taccagtgct
    16561 ggatagagtc atttaatcta ttttcagtat cctatctatt atattattta ccccttaatt
    16621 tgctatccaa tgcctattgt atctatatat atctttttta attacatctt tatttcttct
    16681 tttttctctg taacttaccc cctatactct cgtatttcaa aaacaaataa aatcatcagc
    16741 tttttataag cttgctctta gctttcccct ggtatcaagt gtttatcaag cgttaatcaa
    16801 gccttaatta attaacgctt gatatagaag tgattaacac tagactctta tagatatcta
    16861 ataatccacc ttccttatca attttaactt attgccctaa cataaattat aatttaacta
    16921 aaggaaaagg atagataaat ttatatttgg tcctatcttc tttaacgata gaaaagtacg
    16981 acctatattg ttttgaatta aaaagcaata gaccttaatt tttcgtttat agctcgtaaa
    17041 gataaaccat ctttatgata acgaggaata aaatgtagat ggaaatgaaa aatgctttga
    17101 tgtgcagcgg aaccgcaatt catcagtaaa ttataagctt ctggatgtaa ttcttcatct
    17161 aaaagatttc gcataattcg gctaatttcc aataaagcgg tcatttcttc aggttgaagt
    17221 gtaaaaaaat caggagtgtg cctttttgaa atgatgagag aatgaccctt tgttattggg
    17281 tgtatgtccc ggattgcaaa gaaatattcg ttttccgcta aatatttgtc tggactgata
    17341 agacaaaaaa tacaattcat ttattcttta ccccctttat gtaaactcat aatttaacaa
    17401 aaatctaaat atcaatataa tctgttctac aaaaagagtc aaaggatttt ataatggttt
    17461 attgtggata agaaaaaaat ttgcaaactg atacaggttt aagagataga aaaaatttta
    17521 ttgacagatt agttgtttat atgaatgaga aaataaatag ggatttaaca ttccttgagc
    17581 ctgttccttt gttcgggaaa gcctcaatct gaatgtaaga tgtcccgaat gcaaaggagt
    17641 acaaaatgcc tgacaagact ttaatctgca aggactgcca gaaagaattt gtgtttacag
    17701 aaggtgagca agaattctat caagaaaaag gtttagttaa tgaacctcag cgttgtccag
    17761 aatgccgtaa agcaaaaaaa gccagatata accgtaaccg ttatcaacac aacgctcgtt
    17821 agaattaaat ctggttatta aaaaaccgct gtctatcccg atagcggttt ttcgttttat
    17881 ttttaaagag tttcttcaac cctgctttaa attgcaggga atttgaaaag ttattggctt
    17941 agggaacggt cacttgcgta actttattgg gctcaataac aatatcatct gccagtaata
    18001 tcctattttt agacctgatt tccaaatgat gagttcccac tcttaaagga attgtattgg
    18061 catcatattc tgggtctatt cccacactct gtccatcaac ataagcctct ctattgacca
    18121 tacttccttc cagacggaga tatccatact gtacttgctc tttgtcctta ttggcactac
    18181 aggagaataa aaatattaat aagagcaaag taatgcataa tctaaagata gttttattca
    18241 attcttctac ctcaacttgt ttttttatat taaattccag caaggtagac tctgtcaaga
    18301 agaagagtta aatttattag aaaatagaat aaatcccttc taaagaaaat aatttaacca
    18361 ttaaaaaagc ctatcccgat tgaatcagga taggctttta actaattgtg atatttttaa
    18421 tccctttctt tcccggttaa gcactccgga aaatagagg
//
LOCUS       DAORZF010000032        18450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_2494_length_18450_cov_372.099701, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000032 DAORZF010000000
VERSION     DAORZF010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 18450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18450
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2494_length_18450_cov_372.099701"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2313
                     /gene="priA"
                     /locus_tag="PK015_04955"
     CDS             <1..2313
                     /gene="priA"
                     /locus_tag="PK015_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOL33849.1"
                     /translation="PQEIPEGVRVLVSFNRKDMIGICGTRFESAPEKDIRYKQIIEVL
                     DQQPLIDAQLMSLAKWMGEYYYCSTGTACFAMLPAWLIPDIQAEVRWVADNIPEKYKE
                     LANAFSEGQAQKVSELRKKLKGKPVLQWVEKATEEGYLELNRKLSPKDKPKTVNYIHI
                     IDKTVDPNNLPSKQKEALQLIYAKSKEEFPLAEISRDVTYSVIHALERKGIISIFPRK
                     IEREFFRFETSGLKKVVTLNEEQERAIQEILQHKGHFQVHLLYGITGSGKTEVYIPII
                     RSYIQEGKGVIFLIPEIALTPQMVERFQAEFGDILAISHSQLSDRQRLEQWQRIAKGK
                     CRLVIGARSAIFSPMPNLGLIIVDEEHEQSYKQENNPRYNGRDVAVMRGKISDAQVIL
                     GSATPSLESWYNQKKGKYLLHCLNSRPLDIKLPQVQIVSLCEDYSQQMFSDELISAIS
                     FRLERNEQVILFQNRRGFSSYMQCLKCGELIKCPNCDISMYYHRDREEMQCHYCGNTY
                     PSPRKCPQCGSYSFVYGSPGTQKVEQTLRILFPQARILRLDSDSARQQDIYKIMYRRM
                     KDKEVDILLGTQMISKGLDFPEVTLVGIVNADISLNIPDFRAAERTFQLCTQVAGRSG
                     RADKEGEVIIQTYNPRHYAIIYASKQDFPGFAEEELSHRRHLYYPPYYRLARILFQSA
                     DNNLLQEEMDELSQKVKNLLNTFPQGEIFLLGPAPAPFTKMKNLYRWHLIIKGRTTQS
                     IKKAIDAVLNEYKVSSTIHYTIDIDPTTLM"
     gene            2379..2702
                     /locus_tag="PK015_04960"
     CDS             2379..2702
                     /locus_tag="PK015_04960"
                     /inference="COORDINATES: protein motif:HMM:NF018633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HOL33850.1"
                     /translation="MEKKEKGFEDLTIPEDLEGEDYSDRYLTLEMDDGTQQDFIVLDI
                     FEYEGSQYIALADKDTLQYDILSMNFINDEEVELNFIEDEDKFNDIADWFEKLYYNVE
                     EEPED"
     gene            2765..4015
                     /gene="hutI"
                     /locus_tag="PK015_04965"
     CDS             2765..4015
                     /gene="hutI"
                     /locus_tag="PK015_04965"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HOL33851.1"
                     /translation="MQVDTIVRNCRQLITCKGKDGPLKGKEMSDIVIFEDGALAIKDG
                     YIVDIGSDAEIAEKYTADEIISAKNYLVLPGFVDCHTHPVFVHTREDEFALRLQGYSY
                     MQIAKAGGGILNTIKTTRNASEELLFDLAKKRIEKMMELGTTTLEAKSGYGLDTESEL
                     KMLRVIDRLAKELPVTIVPTFLGAHEYPEEYKNNHEGYLDLLCNEMIPAVAKQGIARF
                     CDIFTEADVFSIEESRRVLNCAKQYGLQLKMHADEIEPLGGAELAAELGCISADHLGS
                     TSDAGIKALKDKGVIPVLLPATLFSLGSKTYARGREMIDMGLPVALATDYNPGSCNCD
                     SIPLTMSIACIQMKLIPVEAFCATTVNAAYALGLGDKVGSLEIGKQADFTIWDIPSLN
                     FIPYHLGSSYIVSVYAKGKCIYKA"
     gene            4019..5941
                     /locus_tag="PK015_04970"
     CDS             4019..5941
                     /locus_tag="PK015_04970"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HOL33852.1"
                     /translation="MNDTTNGIAYTIVQTLHRSRKYWVYKARDNNTGKFVILKANDIQ
                     WRNDPTCIQALREESEAGLKLNHPYIRKTIGLFEDDGAYYLVSEFIEGETLDAILAQN
                     HFKISYELAHKWTLQLLEAINYAHQCGILHLNLNPANIFITNSQDIKISGFGKDPQSW
                     KYANPEESKYHPILFTAPEVFLERKPYDFRADIYSIGAIAYLLFCGQLPWSLDPRLSP
                     SAQKSKTLEQIVINPNLLGKLIPEWLFKILNKALMIDPQLRFNNAEEMHSAIISELDI
                     PYIPSTTKAEAISSQTTLTKETILPSQSESSYSKKQNLSESDLSEKEKSVKSSSKKDN
                     QEQQIVITTDPSLTKMQKTLKIIGIISAGILIYIIVKYVIIKGIQNSIKAKQESSVVT
                     PYVTEGTVRNQLLELNAVEGDSTIIGSMEPDADPDEFPPHTVFIPSFYISPYEITMEQ
                     WAMVFPSSTLKDEERDLPVTNVSFDEVIQFCNEKSLLDGLQPCYEYVDNYIVCDFQAS
                     GYRLPTEAEWEFAAKEGKYETPYTYSGSNNIDQVAWYIENSGGHSHPVGEKKYNKLKL
                     FDMSGNVSEWVWDWYAPYSANVPFSFTGPESGTDKVIRGGSWKDPAREARITNRAYAK
                     PYSKTNYIGFRVVRSK"
     gene            complement(6185..6766)
                     /gene="nadD"
                     /locus_tag="PK015_04975"
     CDS             complement(6185..6766)
                     /gene="nadD"
                     /locus_tag="PK015_04975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HOL33853.1"
                     /translation="MSNLALLGGSFDPVHNGHLHIARGILHSGLVESVIFLPNAIHNF
                     KKDCIVLDFNSRYELVKEVMEPGMEVWDDDAKGTGYTSDLLQHLYKKYPEHQFFWVIG
                     SDNLASLSRWHNYPWLKENVQFLIIPRPGYPIIPEVINEIKHQVLNLTPIDISSTQIR
                     NNIAAGLSIKGLVPDSILDKVLQLYKPLLAPKT"
     gene            complement(6763..7485)
                     /gene="bamD"
                     /locus_tag="PK015_04980"
     CDS             complement(6763..7485)
                     /gene="bamD"
                     /locus_tag="PK015_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOL33854.1"
                     /translation="MHKILTILLVILTVITACSSNKTVLTTEEKLSQADQLYAKGKYS
                     RAAELYAQVYMDRISTASAYALLREADCYFKINRFADAYSAYQEYIINFPNYPEVSTA
                     LFRSAVCLYEQSLGPQYDQTETIMAIEAFEKFISKYPNDPRCNEALQYIQKAKYRLTE
                     KKFHIGYTYYKMRDYSAALMYFKEIIAEGNTNSLDRQSLYYATLILHKQKLDDQALVY
                     YNTLKAKYPGSKESKKLEKYFK"
     gene            complement(7495..8079)
                     /locus_tag="PK015_04985"
     CDS             complement(7495..8079)
                     /locus_tag="PK015_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33855.1"
                     /translation="MSKSSLIIILALMGFCLCAETHYPLAEPEIISTQIVNLIDLSEP
                     IFLDIQAGDFTSALDYHIRYMLLEKGADIREWNAENNPFYLPSLTDSIPKSLLPLSLA
                     KAHLVIITMDLGSTKVETKSFLSYHSESYPLYSFQIKQIELPSYKLLRVDNLSFTDSK
                     IRSSNSNITSLKWFEPILAVTAISSIIYLLWTIE"
     gene            complement(8072..9355)
                     /locus_tag="PK015_04990"
     CDS             complement(8072..9355)
                     /locus_tag="PK015_04990"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOL33856.1"
                     /translation="MKKITIILLCALMLILCSCVSNKTFKAQEEKVQILQARQNNQDV
                     QLEMINKELANHREKLNDIMVQLSIINQQLPEIDNLKQVTDQSTANIAKLQNQINQIN
                     EQVTEVMNSINSLDIRMAQLYTETNDVFDAYSDYLKQMKDAQKNMATKEEVAILIAET
                     SAWSESLVKLFSQVEALSSQLTEQQSLVAELMTLQSKVASSEQENEEIKNEITKINTT
                     LAEQEHIKQELVAIHQSLLTAQKEIASLQKELNQIQSAQPASSSEVNDLRARVLAISK
                     DLDALTSDLQMVIAQERAAAEKRRNEARDKQYKTALNEYYKGNFEKSILLFEEFLTSY
                     PDSELVPNAHYWIGENYYSAKNYPKALREFQIVVSNYPGHQKAWDAQLKIGLTYYQMN
                     DFKSAYQELMVIKNQNPAYPQMPIVDKYLSKLKDV"
     gene            complement(9379..10794)
                     /locus_tag="PK015_04995"
     CDS             complement(9379..10794)
                     /locus_tag="PK015_04995"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOL33857.1"
                     /translation="MQAKKEQVNQLLYERKNFWKEASAEEIKKAFDFAVPYKDFLNQC
                     KTERETVIWTEELLKANKFKALPAAKQNKKVYSVFRNKTIAMAVLGSEPVSAGFNMVA
                     AHIDAPRVDLKQNPLYEDKSSAVACMKTHYYGGIKKYQWVSTPLAIHGTIIKSDGTTI
                     NISIGENNEEPVFIIPDLLPHLARKEQYTKTLGEAISASNMNLIFSGMMEPKTEEKEA
                     IKLYALKLLNQRYGIQEKDFISAELELVPATSARDAGLDASMVVGYGQDDRICAYSGL
                     QAMLSAMDKKPKRTMVVYFSDKEEVGSQGNTGAQSVFLQDFIAALLAHNGESGSSSNL
                     RKTFMNSRVLSGDVTAAVDPNYPNVHELQNAVIFNHGVGISKFTGSGGKSGCNDANAE
                     FVAQVIRIFDNAGVFWQMGELGKVDEGGGGTIAYILANLGAEVVDCGTGLMGMHSLYE
                     LSSKADLYSTYAGYKAFLISQ"
     gene            complement(10811..11290)
                     /gene="greA"
                     /locus_tag="PK015_05000"
     CDS             complement(10811..11290)
                     /gene="greA"
                     /locus_tag="PK015_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOL33858.1"
                     /translation="MDLSTLITQEGMDRLQRRIQELLRERPAVIKAVATAREYGDLSE
                     NAEYKSARERQRQIDTEIDYLRRRAAHLKVIDISTIPKDRVRFGAICRIRDEANGEII
                     VYQIIGADELNFFDKEGIQPVSVLSPIGNSLLGKQVGDKVVVDAPMGERILEILEIS"
     gene            complement(11292..12104)
                     /locus_tag="PK015_05005"
     CDS             complement(11292..12104)
                     /locus_tag="PK015_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33859.1"
                     /translation="MLTIDWKERLNKDTADYVENKLPKNDYDFEIIYNAYPERVNGKI
                     PNEVIVFVANALVNKIGKNHSQFIPFYKHLWDKKGENGKLAFIIIMSKLLAKNPSLYL
                     PLIDTAVNTASENELIILMEKVMLPQLRKNPDQFLPQVYKWTTSHKEKIRKQAINLLI
                     KLMRKDPHLIDEIVQHFLNQWYHPLGELANNHITLLKAVAKLSPDAYLNIWRQFNMSR
                     DPQIAELLCSSITFYHPEIEQTVERWTKSGNARLKRAALAAQKLLQKKKSQA"
     gene            complement(12175..15384)
                     /locus_tag="PK015_05010"
     CDS             complement(12175..15384)
                     /locus_tag="PK015_05010"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOL33860.1"
                     /translation="MARLFSKDYHEKSASWYFNHVVIPLENGDLEYWQSIDEKGCYHI
                     HFVATPDKREFFNTQLDVIYDPETQRIISHHCHECKDDESCRHYLSVLRYAYMNLRTD
                     IFEQPAVETCDGTVLRGREKWLDIYDKAALEIEGIYAPDVDKVRLYHDSYEPLNISLL
                     IRIQAGADIEGITEQEKKELLSQLEVFSDTEQALFKFLNANKAAYSSKAKFWSLYKKD
                     FPRALGYLQALNGKVKVRETGEPLKFSSEPYPLSLRIEPAGKVNYRVYPVIIDELSAV
                     YSGYPTWLFFRNVVHPVYLPLTNENIDRLFNQELFISAKDLIYWSTVVHKQLHKQGIY
                     LDFDPSIELPEIISTEPRIKFKVFPMGEDYILEGKLVYPSPYLEEGGKLEIPLSTIRF
                     QAPLIRSDYISEEETGNAWFYIPPHIFDQAKELFEKLPEANKSRLEQFSQLIYEGEEG
                     LDKLRKSLFELESSAWDLEIVPELRGNFIQKVDLQVEIKARRSEEIDWFSYEVSYHYK
                     DLSFTYEELNRFFRSKEEFLHTKDGRILYIVNPEVFFESSRLIKHSERLADNVYRARL
                     IELPYYHRYMQENPAFRVLGDDFLTQLGIDLKRGHLEHTEPLPLYLQTIMRGYQKAGY
                     AWIKMLEYYHLGGILADEMGLGKTIQALAAIQNAPENTQSLVVCPKTLLYNWAAEIDK
                     FHINIPYLIVEGDKETRVQLLQNPNVKLFIISYTVVLNDIDILKDKKLHWIILDEAQN
                     IKNVNAKRTYAIKKIAAEHRLALTGTPVENNLTELWSIYDFLMPGYLGTLKRFKKDYI
                     DDTENGPARLRRIVAPFLLRRVKKEVLLELPDKQEQVSWCKLHPVQEKLYLQVIDTVQ
                     KKLLHSPEAAQLNFVNVLADLTKLRQICNHPHLADPDILPEPEASSKLELLIELVEDA
                     MGSGHKILVFSQFVQMLKIIRQVFDNMNLNYAYMDGKSKNRLQEVRRFENNPELQLFL
                     ISLKTGGTGLNLTAADTVILYDPWWNPMVENQAIDRTHRIGQTHKVQVYRLITKGTVE
                     EKIMNLQQHKLQLFDEVVSDTRKFLSNLTPEEIRDLFTY"
     gene            15646..16698
                     /gene="wecB"
                     /locus_tag="PK015_05015"
     CDS             15646..16698
                     /gene="wecB"
                     /locus_tag="PK015_05015"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOL33861.1"
                     /translation="MKIAQVVGARPQFIKLAPLSRLVREKHEEIIIHSGQHYDIQMND
                     VFFQDMLIPAPDYNLDVGSGTQAQQTAEILEALEPVLIEEKPDWVLVYGDTNTTLAGA
                     LAAAKLGLKTAHIEAGLRSFNRSMPEELNRIVADHLSDLLFCPTDAAMNNLAKEGLAE
                     RAYLVGDIMTDSLKLGLELAKDNSDILNELTLNPGTFLLLTLHRPYNVDDPKLLSLIL
                     SGLNSLNKLIVFPVHPRTRNILCQIEPDKFNNIRFIEPLSYLDFLTLMQASEMILTDS
                     GGIQKEAYIIGKLCITLRPETEWIETVKTGWNILLPPQSKEFPEKILSISKPSSHPPL
                     FGSDVSQKILSILEQT"
     gene            16717..17394
                     /gene="udk"
                     /locus_tag="PK015_05020"
     CDS             16717..17394
                     /gene="udk"
                     /locus_tag="PK015_05020"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HOL33862.1"
                     /translation="MEKQPDFSPKTRENPSENLAPKAHLILIGGGSCSGKTTIALAIG
                     RRITGLKTVIISQDSYYKDLSALPFEERTKVNFDHPDAIDIDYLLSDLEKMMKGEPVN
                     VPSYDFVTHTRQEGTLCIAQAEVIILEGIFALYYPELLEMSDLKIYLDSDADIRLARR
                     LQRDILERGRSVESVLNQYLETVKPSHQAFIEPTKKNADIIIPGEKEFDKVLIMLNGY
                     LQHEFLY"
     gene            17542..17814
                     /locus_tag="PK015_05025"
     CDS             17542..17814
                     /locus_tag="PK015_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOL33863.1"
                     /translation="MKLKPIEDHIVVKVPTAVEEKTVGGIIIPDTAKEKPQIGEVIAV
                     GTDEDLQKIVKVGDKVLYGKYSGTEVEVEGEKLLILSKDDILAIVE"
     gene            17818..18135
                     /gene="trxA"
                     /locus_tag="PK015_05030"
     CDS             17818..18135
                     /gene="trxA"
                     /locus_tag="PK015_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOL33864.1"
                     /translation="MLELNSQNFEAEVKQSELPVMVDFWAPWCGPCKALSPVVEKLAE
                     ELAGKVKVMKCNIDECPEIATRFSIMSIPTLLIFKDGKVVEQLIGLVQKDKILDKLRP
                     YLS"
BASE COUNT         5760 a         3357 c         3540 g         5793 t
ORIGIN      
        1 cctcaggaaa ttcctgaagg ggtgagagtg ctggtctcct ttaacaggaa agatatgata
       61 gggatctgtg gaactcgttt tgagagcgct ccagaaaaag atattcgcta taaacagatt
      121 attgaagtct tagaccagca accactaatt gatgcccaat taatgagtct ggcaaaatgg
      181 atgggagagt attattattg ctccactggt acagcttgtt ttgcaatgtt acctgcctgg
      241 ttaattcctg atatacaggc tgaagttaga tgggtagcag ataatattcc agaaaaatac
      301 aaagagctgg ctaatgcttt ttctgaaggt caagctcaga aagttagtga gctgcggaag
      361 aagctaaagg ggaagccggt gcttcaatgg gtagagaaag caacggaaga agggtatctg
      421 gagctaaatc gtaaactcag tcccaaagac aaacctaaga cagtcaatta tatacatatt
      481 attgataaaa cggttgatcc caataatctt ccttccaagc agaaagaggc tttacaattg
      541 atttatgcca aatcaaaaga agaatttccc cttgctgaaa tatccagaga tgtaacctat
      601 tccgtaatcc atgctttgga acgtaaaggg ataatcagca tatttcccag aaaaatagaa
      661 cgggagtttt tccgttttga aacctcgggt ttaaaaaaag ttgtaactct caatgaggag
      721 caagaaagag ctatccagga aattttgcag cataagggac attttcaagt tcatctactt
      781 tatggtataa caggtagtgg taaaactgag gtttatatac ctatcattcg ttcttatatt
      841 caggaaggta agggggtaat ctttttaatt cctgagattg ctctcacacc tcagatggta
      901 gaacgcttcc aggcagaatt tggcgatatt ctggctatca gtcacagtca attaagtgac
      961 cgtcagcgct tagagcaatg gcaaagaatt gctaaaggaa aatgtagatt agttataggt
     1021 gctcgcagtg ctatattttc accaatgcca aatctgggtc tgattattgt tgatgaagag
     1081 catgagcaat cttataaaca ggagaataat cctcgttata atggcagaga tgtggcagta
     1141 atgagaggaa aaattagcga tgctcaagtt attttaggaa gtgcaactcc ttctctggaa
     1201 tcctggtata atcaaaaaaa aggtaaatat ctattacatt gtctgaatag tcgtcctctg
     1261 gatataaaat taccccaggt gcagatagta tccttgtgtg aagattacag tcagcagatg
     1321 ttttccgatg aattaatttc tgccatatct ttcaggctgg aaagaaacga acaggttatc
     1381 ctttttcaaa accggcgtgg cttttcatct tatatgcagt gtctaaaatg tggggaacta
     1441 atcaaatgtc ccaattgtga tatcagtatg tattatcatc gtgaccggga ggagatgcaa
     1501 tgtcattatt gtggaaacac ttatcccagc ccaagaaaat gtccccaatg tggtagttat
     1561 agctttgtct atggttcacc aggcacgcaa aaagttgagc aaactcttag aatactattt
     1621 cctcaagcca gaatattacg tctggattca gattcagcca gacaacaaga tatctacaag
     1681 attatgtatc gcaggatgaa agataaggaa gtagatatcc ttttaggaac tcagatgata
     1741 tctaaaggat tggattttcc agaggtgacg ctggtaggta tcgtaaatgc agatatcagc
     1801 ttgaatatac cagattttag agccgcagaa cgcacttttc agctttgcac tcaggtggca
     1861 ggtcgttcag gtcgtgctga caaagaaggt gaggttatta ttcagaccta taatcccagg
     1921 cattatgcta taatttatgc cagtaaacaa gatttccctg gatttgctga agaggaatta
     1981 tctcatcgta gacatctcta ttatccacca tattatcggt tagccaggat attatttcaa
     2041 tcagcggaca ataatttact gcaagaggaa atggatgagc tctctcaaaa ggtaaaaaac
     2101 ttgctcaata cttttcccca aggagaaata ttccttctgg gacctgctcc tgcacctttc
     2161 actaaaatga agaatcttta tcgttggcat ctaattatta agggacgcac aactcaatct
     2221 atcaagaagg caatagatgc ggtccttaat gaatacaaag tttcctctac tattcattac
     2281 acaatagata ttgatcccac tactttaatg tagagcaaga aataattaac ttgccaaaat
     2341 caatcatttg taaaataaga acaaaaaagg agtttattat ggaaaaaaag gaaaaaggct
     2401 ttgaagacct aaccataccg gaagatttgg aaggtgaaga ttattcggac cgctatctga
     2461 cattggaaat ggatgacggc acccaacagg attttattgt cctggatata tttgaatatg
     2521 aaggtagtca atacattgct cttgcggata aagatactct gcaatatgat atcctatcta
     2581 tgaattttat aaatgatgaa gaagtggaat tgaatttcat tgaggatgag gataaattta
     2641 acgatatagc agactggttt gaaaaattat attataatgt agaagaagaa cctgaagatt
     2701 aatcagaatt gaaccccata ttagcattat attcttttaa caaataggga gaagacagag
     2761 aaaagtgcag gttgacacca tagttagaaa ttgcaggcaa ctcataacct gtaagggtaa
     2821 agatggacct ctaaagggaa aagaaatgag cgatatagtc atctttgaag atggtgcatt
     2881 ggctataaaa gatggctata tagttgatat tggaagtgat gcagagattg cggagaaata
     2941 tactgctgat gagattatat ctgccaaaaa ttatcttgtt ctgcctgggt ttgtagattg
     3001 tcatacgcac cctgtttttg ttcacactcg cgaagatgaa tttgctttgc gtttacaggg
     3061 atattcttat atgcagattg caaaagcagg tggaggtatt ctaaatacca taaagactac
     3121 ccggaatgcc agtgaggaac tactttttga cctggcaaaa aaacgcatag aaaaaatgat
     3181 ggaactgggg acaaccactc tggaagcgaa aagtggctat ggcttggata cagaaagcga
     3241 attgaaaatg ttaagagtta tagaccgtct ggcaaaagaa ttacctgtaa ctattgttcc
     3301 tacattcctg ggagcacatg agtatccaga agaatataaa aataatcacg aaggatatct
     3361 ggacctttta tgtaatgaga tgataccagc agtagccaag caaggtattg ccaggttttg
     3421 tgatattttc acagaagctg atgtctttag cattgaagaa tcccgcagag tgcttaattg
     3481 tgccaaacaa tatggtttac agttgaaaat gcatgcggat gaaatagaac cattgggagg
     3541 agcagaactg gcagctgaat taggatgtat ttctgctgac catttaggtt caacttcgga
     3601 tgctggaatt aaagctttaa aagataaagg tgtgataccg gttttacttc ctgctacttt
     3661 attttctctg ggaagcaaga cctatgcaag aggcagggaa atgatagata tgggtctacc
     3721 agtagcttta gccactgatt ataatccggg gagttgtaac tgtgattcaa ttcctctaac
     3781 tatgagtatt gcctgtatcc agatgaaact tatccctgta gaagcatttt gtgccactac
     3841 tgttaatgct gcttatgctc ttgggttagg agataaagtg gggtccttag aaattggaaa
     3901 gcaggcagat tttactatct gggacatacc atcgcttaat ttcattcctt atcatcttgg
     3961 aagttcctat attgttagcg tttatgcgaa aggcaagtgc atctataagg cttaatcaat
     4021 gaacgatacc accaacggta ttgcttatac tatagttcaa acccttcata ggtctcgtaa
     4081 atactgggtt tataaagccc gggataataa tacgggtaaa tttgttatct taaaggcaaa
     4141 tgacatacaa tggcgtaatg atcccacttg tattcaagct ttgagagaag aaagtgaagc
     4201 gggattaaag ctgaaccatc cctatataag aaagactatt ggattatttg aagatgatgg
     4261 tgcatattat ctggtaagtg aatttataga aggggagacc ctggacgcaa tccttgctca
     4321 aaatcatttt aaaatatctt atgaactggc tcataaatgg actttgcagc ttttagaagc
     4381 catcaattat gctcatcaat gtggtatctt acatttaaat cttaatccag ctaatatatt
     4441 tatcactaat tctcaagata taaaaatctc tggttttggt aaagacccac aaagctggaa
     4501 atatgccaat cccgaagaaa gcaaatatca tccgatatta ttcaccgcac cagaggtatt
     4561 tttggagcgt aaaccttatg attttagagc tgatatctat tctattggag ctatagctta
     4621 tcttcttttt tgtggtcagc ttccctggtc tttagacccg cgtttaagtc cttctgctca
     4681 aaaaagtaaa actctggaac aaatcgttat aaatcccaat ttattaggta aactgatacc
     4741 tgaatggcta tttaagattt taaataaagc cctgatgatt gaccctcagc ttcgttttaa
     4801 taatgcagaa gaaatgcact ctgctattat ttcagagctg gatatacctt atattccctc
     4861 tacaactaag gctgaagcaa tcagttcaca aacaactttg acgaaagaaa caattttgcc
     4921 ttctcaaagt gagagttctt attctaaaaa gcaaaatctt tctgaatcgg atttatctga
     4981 gaaagaaaaa agtgtgaagt cctcttctaa aaaagataat caggaacaac aaattgtcat
     5041 aaccactgac ccttctctga caaagatgca aaagacatta aaaataatag ggattatatc
     5101 cgctggaatt ttaatctaca ttatcgttaa atatgttatt atcaaaggta ttcaaaacag
     5161 tatcaaggca aaacaagaga gttctgtggt tacaccatat gtaacagaag gtacagtacg
     5221 caatcaactt ctggaattaa atgcggtaga gggagattct accataattg gttctatgga
     5281 acctgacgca gatccggatg aatttccacc tcataccgtt tttattccct cattttatat
     5341 cagtccctat gaaataacaa tggaacaatg ggctatggtt ttcccgagta gtacattgaa
     5401 ggacgaagaa agagatttac ctgtaactaa tgtatctttt gacgaagtta tccagttttg
     5461 taatgaaaaa agtttgttag atggacttca gccctgctat gaatatgtag ataattatat
     5521 tgtctgcgat tttcaagcct caggatatag gcttcctact gaagcagaat gggagttcgc
     5581 cgccaaagaa ggaaaatatg aaacacccta tacttatagc ggttctaata acatagacca
     5641 ggttgcctgg tatatagaaa atagtggagg acattctcat ccagtgggtg agaaaaagta
     5701 taataaactg aaactatttg atatgagcgg caatgtgagc gaatgggtct gggattggta
     5761 tgcaccttat tcggctaatg tgccattttc ctttacaggt cctgaatctg gaacggataa
     5821 agttattcgg ggtggttcct ggaaagaccc tgccagagag gcgagaataa ccaatcgcgc
     5881 ctatgctaaa ccctatagta agaccaatta tatcggtttc agagtggtac gaagtaagta
     5941 agatatctta aaatatcccc tgtttgtttc ctgtcactca cttagcaaaa gagctaaggg
     6001 agagccaaga aagagctaag agagaagttg taatctattg caatataatt tgatatgcga
     6061 tttagatggt ttaagtgcat agtaataaat aaaatagata tgattttcct aaggattaga
     6121 ggggattaaa cggattattt tctcaaatat attattactt ctttaggtaa taaattaaca
     6181 ataattaagt tttaggggca agtagcggtt tgtatagctg taaaacctta tccagaatag
     6241 agtcaggcac cagacctttg atggataatc ccgcagctat attgtttcgt atctgagttg
     6301 aagatatatc tattggagta agattgagca cctgatgttt tatttcattt ataacttctg
     6361 ggatgatagg ataaccaggt ctgggaataa tcaggaactg cacattttct tttagccagg
     6421 gatagttatg ccagcgagaa agagaagcca gattatcaga gcctattacc cagaagaatt
     6481 gatgttcagg atatttctta tataaatgtt gtaataaatc tgaagtgtag ccagttcctt
     6541 tagcatcgtc atcccagact tccattcccg gttccataac ctcttttacg agttcataac
     6601 gagaattaaa atccagcacg atacagtctt ttttaaaatt atgaatagcg ttgggaagaa
     6661 aaattacgct ttctactaaa ccggaatgaa ggattcctct ggcgatatgc aggtgtccat
     6721 tatgaaccgg gtcaaagctt ccacctaata aagccagatt gctcatttga aatatttttc
     6781 taattttttg ctctctttag aaccgggata ttttgcttta agggtattat aatagacgag
     6841 agcttggtca tccagttttt gtttatggag gattaaagtt gcataataga gagattgtcg
     6901 gtccagacta tttgtatttc cttcagctat aatttcttta aaatacatca aagcagcgct
     6961 gtaatcccgc attttatagt aagtgtagcc aatatggaat ttcttttcag tcagtctata
     7021 ttttgctttc tgaatatatt gaagagcttc attgcaacgc gggtcattcg gatacttgct
     7081 tataaatttt tcaaaagctt caatagccat tattgtctca gtttggtcat actgcggtcc
     7141 taaagattgt tcgtaaagac agacagcaga acggaaaaga gcggtgctaa cttcgggata
     7201 attaggaaaa ttaataatgt attcctgata ggcggaataa gcatcagcaa agcgattgat
     7261 tttaaagtag caatcagctt cacgcaaaag tgcataagca gaagcagtgg atattctatc
     7321 catgtagact tgagcataca attctgcagc acgagaatat tttcctttgg cataaagctg
     7381 gtctgcttga gaaagctttt cttccgtagt caagacagtt ttatttgagg aacaagcagt
     7441 tattacagtt aaaatgacga gtaaaatagt aagtatttta tgcataataa taccttattc
     7501 aatagtccag agcagataaa ttattgagga tatagcggtt acagccagta taggttcaaa
     7561 ccatttaaga gaagttatat tggaattact ggagcgaatt ttggaatcag taaagcttaa
     7621 gttatccact ctgagaagct tataggaggg cagctctatt tgctttatct gaaagctata
     7681 gagaggataa ctttcgctgt gataactcag aaaactttta gtttctacct tggtgctgcc
     7741 taaatccata gtaattatta ccagatgagc tttagctaag gagaggggta ataaagattt
     7801 agggatggaa tcggttaatg aaggaagata aaaaggatta ttttccgcat tccattcccg
     7861 gatatcagca cctttttcca gcaacatata cctgatatga taatccagtg cagaggtgaa
     7921 atcaccagcc tgaatatcca gaaagatggg ttcggaaagg tctatgaggt tgactatctg
     7981 agtgctaata atttcaggtt cggcaagtgg ataatgggtt tctgcacaaa ggcagaaacc
     8041 catcaaagcc aaaattatta tcagcgagct tttagacatc tttaagctta ctcagatact
     8101 tatccactat aggcatctga ggataggcag gattttgatt tttgataacc atcagttcct
     8161 gataagcaga tttaaagtca ttcatttgat agtaggtgag acctattttc aattgtgcat
     8221 cccaggcttt ttggtgtccg ggataatttg agaccacaat ttgaaattcc cgcaaagctt
     8281 taggataatt ctttgcacta taatagtttt cacctatcca gtaatgtgca ttaggaacaa
     8341 gttctgaatc tggataggaa gttaagaatt cttcaaaaag gagaatagac ttttcaaaat
     8401 tgcctttata atattcattc aatgcagttt tatattgttt gtcccgagct tcattacggc
     8461 gtttttctgc tgcggctctt tcttgagcta taaccatttg taaatcggaa gttaaggcat
     8521 ccaggtcttt actgatagcg agaactcttg cccgaaggtc atttacttca gaagaacttg
     8581 ccggttgggc actctgtatt tgattaagtt ctttttgcag agaagctatt tctttctgcg
     8641 ctgttaataa agattgatga attgcaacca gttcctgttt aatgtgttct tgttctgcca
     8701 gagttgtatt aatcttagtt atttcatttt taatttcttc attttcctgt tcagaagaag
     8761 ccactttact ttgcagggtc ataagttcag cgactaatga ttgttgctcg gttaattgag
     8821 aagataaagc ttcaacctgg ctgaacaatt taaccagact ttctgaccag gcagaggttt
     8881 cggctataag tattgctact tcttcttttg ttgccatatt cttttgtgca tctttcatct
     8941 gctttaaata gtcagaatag gcatcaaaga catcatttgt ttcagtatat aattgagcca
     9001 tccggatatc cagggagttg atgctattca taacttctgt cacttgctca ttaatctggt
     9061 ttatttgatt ttgtagtttt gcaatattgg cagtggattg gtctgtgacc tgttttaaat
     9121 tatcaatttc aggtagctgt tgattgatta tactaagttg caccataata tcgttcagtt
     9181 tttcccgatg attggctaat tctttattaa tcatctctaa ctgaacatcc tggttgtttt
     9241 ggcgggcttg gagaatttga actttttcct cttgagcttt aaaagtctta ttactaacgc
     9301 aggaacaaag tattagcatt aaagcgcaga gcaaaatgat ggttatcttt ttcatctaaa
     9361 cctcctgtta tttatttatt attgagagat tagaaaagct ttatatccag cataagtgga
     9421 atataaatct gctttactgg atagttcata aagcgaatgc attcccataa gaccagttcc
     9481 gcaatctact acttcagcac caagattagc cagaatatag gcaatagttc caccaccacc
     9541 ttcatccact ttacccagtt cacccatctg ccagaaaact ccagcattat caaatattct
     9601 aattacctga gctacaaatt ccgcattagc gtcattacat ccagattttc cgccactccc
     9661 agtaaattta gaaattccca caccatgatt aaagataacg gcattttgca attcatggac
     9721 atttggataa tttgggtcta ccgcagcagt aacatctcca gaaagaaccc gagaattcat
     9781 aaaggtcttc cttagattgg agctgctacc agattcacca ttatgagcaa gtaaagcagc
     9841 gataaaatcc tgtaaaaata ctgattgagc accagtatta ccctggcttc ccacttcttc
     9901 tttatcagaa aaatacacga ccattgttct tttcggtttc ttatccatag cagaaagcat
     9961 agcctgtaaa ccagaatatg cacagattct atcgtcctgc ccataaccaa caaccataga
    10021 agcatccaaa cctgcatctc tggcagaagt ggcaggaacc agttccagtt cagcagaaat
    10081 aaaatccttc tcctgaattc catatctctg attaagaagt ttcaaagcgt aaagttttat
    10141 tgcttctttc tcttctgttt taggttccat cataccggaa aagataagat tcatatttga
    10201 agcactgatg gcttcaccta aggtctttgt atactgttcc tttctggcta aatgtggcaa
    10261 caaatcagga atgataaaga ccggttcctc attattttcg ccaatagaaa tattgatggt
    10321 agtcccatca gatttaatga tagtgccatg aattgctaaa ggtgtggaga cccattgata
    10381 tttctttata ccgccataat aatgagtttt catacaggca actgcactgg acttatcttc
    10441 atataaagga ttttgtttca agtccactct gggagcatct atatgagctg ccaccatatt
    10501 aaatcctgcg cttacaggtt ctgaacccag cactgccata gcaatggtct tattgcggaa
    10561 aacagaataa actttcttgt tttgttttgc agcaggaagt gctttaaatt tattagcctt
    10621 aagcagttct tcagtccaga taacggtctc tctttctgtt ttgcattggt tcaggaagtc
    10681 cttgtaaggg acagcaaaat caaaagcttt cttaatctct tctgcggaag cttctttcca
    10741 gaaattcttt cgctcgtaaa gaagttggtt aacctgttct ttctttgctt gcatttatat
    10801 ctccctatat ttatgaaatt tctaaaatct ctaaaatccg ttctcccatc ggagcatcta
    10861 ccactacttt atctcctacc tgctttccta acaggctatt tcctataggg gatagcactg
    10921 aaacgggttg tattccttct ttatcaaaga agttcaattc atctgctcca attatttgat
    10981 agactattat ttctccgttt gcttcatccc gaatgcgaca gattgcacca aaacggactc
    11041 tatccttggg aatggtactg atgtctataa cctttagatg agccgctctt cttcttaaat
    11101 aatctatctc cgtatcaatc tgacgctgtc tttcccgagc tgatttatat tcggcatttt
    11161 cgcttaaatc gccatattct cttgctgtag ccacagcttt aatcactgct ggtctttctc
    11221 ttagcagttc ttgaatgcgt ctctgaagtc tatccatacc ctcctgggta attaatgtgc
    11281 ttaaatccat attatgcttg agatttcttt ttctggagta gtttttgtgc tgccagagct
    11341 gcccttttta gtctcgcatt accacttttt gtccatctct caaccgtttg ttctatctct
    11401 ggatgataga aagtgataga actacaaagc aattctgcta tctgaggatc tctgctcata
    11461 ttaaattggc gccagatatt taaataggca tctggactca attttgctac agccttaagt
    11521 aaagtgatat gattatttgc cagctcaccc agaggatgat accattgatt aaggaaatgc
    11581 tgaactatct catcaatcaa atgagggtct tttctcatca attttatcag cagatttatg
    11641 gcttgtttgc gaattttctc tttatgtgag gtagtccatt tatatacctg aggcagaaac
    11701 tggtcaggat ttttcctcaa ttgaggaagc ataacttttt ccatcaagat tatgagttca
    11761 ttttcagaag cagtattgac cgcagtatct atcaagggta gataaagaga aggatttttg
    11821 gcaagcaatt tactcataat gattatgaaa gccagcttac cattctcacc tttcttgtcc
    11881 cagagatgtt tataaaaagg aatgaactgg gagtgatttt tcccgatttt attaactaag
    11941 gcattggcaa caaagacaat tacttcattt ggtatcttac cattgacgcg ttcaggatag
    12001 gcattataga taatttcaaa atcatagtca ttcttaggga gcttattttc cacataatct
    12061 gcggtgtcct tgttcaatct ttccttccag tcaatggtta gcatctcgtc tccttttaat
    12121 tcctttaacc tacaatgtca atggcttttc aagcagcaaa atgtcaaggg aaatttagta
    12181 ggtgaaaagg tcacgaattt cttctggagt aagattagat aagaattttc tggtatccga
    12241 gaccacttca tcaaagagct gtaatttatg ctgctgaaga ttcataatct tttcctctac
    12301 cgtgccttta gttattaaac gatatacctg taccttatgt gtctgtccaa ttctatgagt
    12361 gcggtctatg gcttgatttt ccaccatagg attccaccag gggtcataaa ggataactgt
    12421 atctgccgca gtaagattta gacctgttcc accagtctta agactgatta aaaataactg
    12481 cagctcaggg ttattctcaa atcttcgcac ttcctgcaat ctatttttgc tcttaccatc
    12541 catataagca taattaagat tcatattatc aaagacttgt cgtatgattt ttaacatctg
    12601 gacaaattgg gaaaagacca gtattttatg tccacttccc atagcatctt caaccaattc
    12661 tattaacaat tcaagttttg aagaagcttc tggttccgga aggatatccg ggtctgccag
    12721 atgaggatga ttacaaatct gacgcaattt agtcaaatct gccagaacat tcacaaaatt
    12781 caattgtgct gcttctggac tgtgcaaaag cttcttctgt actgtatcta tcacctgtaa
    12841 gtaaagtttt tcttgcacag gatggagttt acaccaggaa acctgttctt gtttatcagg
    12901 caattccagt agcacttctt ttttcactct tcggagtaaa aatggtgcaa caatacgtct
    12961 taatcttgcc ggaccgtttt ctgtgtcatc aatgtaatct tttttaaagc gttttaaagt
    13021 tcccaaatag ccaggcatca aaaagtcata aatagaccac aattcggtaa gattattttc
    13081 cactggagtt cctgtaaggg caagacgatg ttcagctgcg atttttttga tggcataggt
    13141 gcgtttggca ttaacattct tgatgttttg agcttcatcc aggattatcc agtgaagttt
    13201 tttatctttt aggatatcaa tatcgttcaa aaccactgtg taagaaatga taaagagttt
    13261 cacatttgga ttctgaagca gctgaacacg agtttcctta tctccttcaa caataagata
    13321 gggtatattg atatggaatt tatctatttc ggctgcccag ttataaagta gagttttggg
    13381 acagactacc agactttgag tgttttcagg agcattttga atggcagcta aagcttgaat
    13441 agtttttccc agacccattt catctgccaa aatccctccc aggtggtaat attccagcat
    13501 ttttatccaa gcataacctg ctttttgata accacgcata atagtctgta gataaagagg
    13561 aagcggttca gtatgttcca gatgtccacg cttaaggtca attcccaatt gcgtgagaaa
    13621 atcatctccc aacaccctaa atgcgggatt ttcttgcata taacgatgat aataaggaag
    13681 ctctatcaaa cgagcacgat aaacattatc cgcaaggcgc tcactatgtt taatcaatct
    13741 ggaactttca aaaaagacct caggattgac gatatagaga attcttccat ctttagtatg
    13801 caggaattct tccttagacc ggaagaaacg attgagttcc tcataagtaa agcttaaatc
    13861 tttgtaatga taagatactt cataactgaa ccaatctatt tcttcactac gtcttgcttt
    13921 aatttcaact tgtaaatcca ccttttgaat gaagtttcct ctcaattcgg gaactatttc
    13981 caaatcccag gcagaagatt ccagttcaaa cagactttta cgaagtttat ccagaccttc
    14041 ttctccttca taaatcaatt gagagaactg ttccaatctg ctcttattag cttctggtaa
    14101 cttttcaaag agttcttttg cttggtcaaa gatatgaggt ggaatataga accaggcatt
    14161 tcctgtttct tcttctgaga tatagtcact acgaattaga ggtgcttgaa atcggatagt
    14221 ggaaagtggt atttccagtt tcccaccttc ttctaaataa ggagatggat acaccagttt
    14281 tccttccaga atataatctt cacccatagg gaaaacctta aatttgatgc gtggttccgt
    14341 gctaataatc tcgggaagct caatagaggg gtcgaaatcc agatatatcc cctgtttatg
    14401 caattgttta tgaaccacag tgctccagta aattaaatct ttagcactaa tgaatagttc
    14461 ctgattgaaa agccggtcaa tattttcatt agtcagagga agatacacag gatgtaccac
    14521 attgcggaaa aatagccagg tgggataacc ggaatatact gccgaaagtt catctataat
    14581 aactggataa acgcggtaat tgaccttgcc agcaggttct atacgcaaac ttaagggata
    14641 gggttcagaa ctaaatttta aaggttcacc cgtttctcta actttgactt tgccatttaa
    14701 agcttgaaga taacccagag ctctgggaaa atctttttta tatagcgacc agaactttgc
    14761 tttggaacta taggcagctt tattggcatt tagaaattta aaaagtgcct gttcagtatc
    14821 ggaaaaaacc tccagctggg agagcaattc cttcttttct tgttctgtga tgccttctat
    14881 atcagctccc gcttgaatcc taataagcaa agatatattg agaggttcat aactatcgtg
    14941 atacaaacga actttatcta catccggagc atatattcct tcaatttcca gtgctgcttt
    15001 atcatagata tccagccatt tttctctccc acgcaaaaca gttccatcgc aggtctcaac
    15061 agctggttgt tcaaatatat ctgttcttag attcatataa gcataacgca gaacagaaag
    15121 ataatgtctg cagctctcat catctttaca ttcatgacaa tggtgactaa tgattctctg
    15181 agtttcaggg tcatagatta catccagctg agtgttaaaa aactctcttt tatctggagt
    15241 ggcaacaaaa tgaatatgat agcatccctt ctcatctatg gattgccaat attctaaatc
    15301 accattctcc agtggaatta ccacatgatt gaaataccag gaagctgatt tttcatgata
    15361 atccttactg aaaagtcgtg ccatctttcc tcttaatttt ttattttaaa tccagttact
    15421 atttcaatct gatttttgtc aatttatttc aatgcaaaga acaaaatatt ttacagaatg
    15481 tcctactgaa gatagttttg cttaatttct gtctcgcttc ttattcaata agattataaa
    15541 ttttaaagat atctttatat tttatttctt tcaccattag caaagcttgc ttgacataaa
    15601 agctttgata aagtaatttg tacccatagc ctaaaaggag tggaaatgaa gatagctcaa
    15661 gtagtgggag caagacctca atttataaaa ttggcaccgc tttcgcgttt agtgcgagag
    15721 aaacatgagg aaataatcat ccatagtggt caacactatg acattcaaat gaatgatgtc
    15781 ttctttcaag atatgctgat tcctgctcca gattataacc tggatgtagg ttcaggaact
    15841 caagctcaac agacagctga gattctggaa gctctggaac cagtattaat tgaagaaaaa
    15901 cctgattggg tgctggttta tggagatacc aatactactt tggcgggagc tttagccgca
    15961 gcaaaattag gacttaaaac tgcccatata gaagccggtc tcagaagttt taatcgctct
    16021 atgccagaag aattgaatcg tatcgttgca gaccatcttt ccgatttact tttctgtcct
    16081 acagatgcag caatgaataa tcttgcaaaa gaaggtttag ccgaaagagc ttatctggtg
    16141 ggtgatataa tgacagatag tctgaaactt gggttggaac tggcaaaaga taattccgat
    16201 attctgaatg aactaaccct gaatccagga accttccttt tactaacctt acatcgtcct
    16261 tataatgttg atgaccccaa gttgctatca ctcattcttt ctggattaaa tagcttaaat
    16321 aaactcatag tatttcctgt acatccacgc actcgtaata ttttgtgcca gatagaacca
    16381 gataaattta ataatatcag attcatagaa cctttatctt atcttgattt tttaactttg
    16441 atgcaagcaa gtgaaatgat actaactgat tccggaggaa ttcagaaaga agcatatatt
    16501 attggaaaac tttgtataac tttgagaccg gaaaccgaat ggatagaaac agtaaaaact
    16561 ggatggaaca ttcttttgcc acctcaatcc aaagaattcc cagaaaagat attatcaatc
    16621 tctaaacctt caagtcatcc acctcttttt ggctcggatg tttctcaaaa gattctttcg
    16681 attctggaac aaacctaaag aaagaagaga tagacagtgg aaaaacagcc agatttctca
    16741 ccaaaaactc gtgaaaatcc ttcagaaaac ttagcgccta aagctcatct gattctcatt
    16801 ggtggaggtt cctgcagtgg gaaaactacc attgctcttg ccataggaag aagaataacc
    16861 gggcttaaaa ccgtcattat atctcaagat agctattata aagatttaag tgctttaccc
    16921 tttgaagaac gaactaaagt caattttgac catcctgatg ccattgatat tgactatctc
    16981 ttgagcgatt tggaaaagat gatgaaaggt gaaccggtta atgtgccttc atatgatttt
    17041 gtaactcaca cgcgtcagga gggaacttta tgtattgctc aagcggaagt gattattctg
    17101 gaaggaattt ttgccctcta ttatccagaa ttgttagaga tgtccgattt aaaaatttat
    17161 ctggattcag atgctgatat ccgtttagcc agacgtttgc aaagagatat tctggaaaga
    17221 ggtagaagtg tggaaagtgt tctaaatcaa tatctggaaa cggtaaaacc ttctcatcaa
    17281 gcctttattg aaccaaccaa gaagaatgca gatattatca ttcccggaga aaaggaattt
    17341 gataaagtgc tcataatgct taatggttat cttcagcatg agtttcttta ttaataaaaa
    17401 agatagatag gatagccaaa tttaggttca aagtttttac tttctgcttg acaaatatta
    17461 gcaatctaaa aagaggtttg ctaataatca cgaatgaatt attatatttt cagaaattca
    17521 atctatatta tggaggaaaa aatgaaactt aaacctattg aagaccatat cgtagtgaag
    17581 gtccctacag cggtagaaga gaagaccgta ggtggaatta ttatcccgga tacggcaaaa
    17641 gagaaacctc agattggaga agttattgcc gtaggaacag atgaagattt acagaagatt
    17701 gtgaaagttg gagacaaggt tctgtatggt aaatattccg gaacagaagt ggaagtagaa
    17761 ggtgaaaaac tgctcattct atccaaagac gatattcttg ccattgttga gtgaacaatg
    17821 ttagagttaa attcacaaaa ttttgaagct gaggtaaaac agagtgaact gccagttatg
    17881 gtagatttct gggcaccctg gtgtggacct tgtaaggctc tcagtccagt agtagaaaag
    17941 ctggcagaag aattagccgg caaggttaaa gtgatgaaat gcaatattga tgagtgccct
    18001 gaaattgcta ctcgcttttc cattatgtcc attccaaccc ttcttatctt taaggatgga
    18061 aaagtggttg agcagttgat tggactggtg caaaaagaca aaatcttaga taagctccgt
    18121 ccttacctta gctaaggaaa aatcttaaaa gatagccccg acaggataaa atcttatcgg
    18181 ggtttttttt gtatccttaa tccgttattt ttgatttatc ggtgaaattt attttgttct
    18241 tgcctcttta tctctatctt ttctccagct tatgtccagc ttatgtccag cttatgtcca
    18301 gcttatgtcc gaaattggtc ataagctact cataaggttg acattaactt ttcatttata
    18361 ggttataaaa gggaaagtgg aaaacaagga taaaaatttt tatagagaat ctgtctataa
    18421 aggagctata atatacatag aatagaggat
//
LOCUS       DAORZF010000033        18429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_2496_length_18429_cov_390.094971, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000033 DAORZF010000000
VERSION     DAORZF010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 18429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18429
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2496_length_18429_cov_390.094971"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1760)
                     /locus_tag="PK015_05035"
     CDS             complement(<1..1760)
                     /locus_tag="PK015_05035"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOL33865.1"
                     /translation="MPSEVILTVENLSLQLGSKVIFAELNLGVHNGDKIGIVGINGSG
                     KSTLLRLLAGNLEPTTGTIVKRRDLTLGWLPQNEPLPAEETILQYVLNQNLSRTSVPE
                     EHRYKAMLSLLNLSDWNKPLSLLSGGERRKAGLAKVLAREPDLILLDEPTNHLDLDTI
                     EWLQDYLTKSRKTLLFVSHDRYFLDAVSNRILEIEHSHCYITEGNYTDYVEQKLIRLT
                     DQKRKEIRRQAQLKKELDWLKRGAKARTSKPKDHINRVQKLISQSYLISKPELKISFL
                     MERQGKTILELHNISKSFEGKLLFQNLEHIFQAGEKIGIIGPNGCGKTTLLKIIAGEI
                     KPDTGKVKVGVNTRFSFFSQEEENYNPDLSVFEYLTQYSEVIQTQSGLKLTAKEMLKR
                     FLFDDKMQQQKLASLSGGELRRLFLLKSLMFGANFIILDEPTNDLDIQTLEILEDYLD
                     DFKGCLLVVSHDRFFLDRCVEQLFVFEKDGIRKFPGSYSDYLLVRNYQKEEKENKKPT
                     LNIAPKSQRQSRGISYKEQRELELLTQEIENLETQIKNLEDSLNSTPTLTHLDYLRIS
                     EELESLNMLYQQKLQRWFDLS"
     gene            complement(1772..1848)
                     /locus_tag="PK015_05040"
     tRNA            complement(1772..1848)
                     /locus_tag="PK015_05040"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1812..1814),aa:Arg,seq:ccg)
     gene            complement(1879..3312)
                     /gene="thyX"
                     /locus_tag="PK015_05045"
     CDS             complement(1879..3312)
                     /gene="thyX"
                     /locus_tag="PK015_05045"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOL33866.1"
                     /translation="MQVNLAGYNIDSSLIRKLQTESATPEVISAAYARISRSEKSIAE
                     LREQALKELEKARRSNQKIIFELGHSSVAEHSVFNFDLIGISRLVTELVETIRFASFT
                     EKSQRYVAFSDDFVIPEELEQPALKLLREEYTQIMQALFNEYKESLKAIQEYYKKVQP
                     QLKKSEIEGKAKEDARYILPLATKTQLGMTINARSLENLLRRLITSPLAEAHELYNLL
                     LQSVKELCPSLIRYIEKEDFKGTFDVAKIKTGITENSFDKSPVKILEFSVDADDKILA
                     ALLFEQCSSSFSSCLETITKLDETKKKELWAEIFEHIQPWSKMPRAFELVDCSFELAM
                     SECCWSQFKRHRVATLLKQKAYSLNNYIFPPIIYTLKREDKWLKLLKETNLLKQKLTE
                     VSPDLVPYARLNAEQVRVLVKMNLREIYHFIRLRADINAQWEIRNLACSFAEYIRPLA
                     PFATSYLCGKSELDSLVQSTGKGLTKT"
     gene            complement(3324..4097)
                     /gene="deoC"
                     /locus_tag="PK015_05050"
     CDS             complement(3324..4097)
                     /gene="deoC"
                     /locus_tag="PK015_05050"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOL33867.1"
                     /translation="MNEIEYIARKLFGDNPPCKCGGGHNICLGCNICRADQPDQLYDP
                     LADIASVIDATLLKADATQSEVNALCDLANEYKCAAVCVNSHFSHPLQLRLNASVKSC
                     TVINFPLGASYIYAVAAEALAVINTGIEELDMVQNLSAVKSGHPLHSYQLIKNIAEMT
                     HSNDVLLKVILETCYLSKEEIITCALYAKKAGADFVKTSTGFGTAGATVENVRLLREI
                     VGPKMGVKAAGGIRTREQALAMLNAGANRIGASNVKALL"
     gene            complement(4094..5140)
                     /gene="rlmN"
                     /locus_tag="PK015_05055"
     CDS             complement(4094..5140)
                     /gene="rlmN"
                     /locus_tag="PK015_05055"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOL33868.1"
                     /translation="MLNSIYGIMPEQLSAFLSSEYPAYRSQQLLKWIYEKKVFDPSKM
                     TDLPATMQSYLSQAFDFSMPQIVQTLKSMDGSQKFRLLLSDGEQIEMVLMPEEKKLTL
                     CVSSQVGCARDCSFCATGKMKLHRNLYPHEIVQQFLLASRETTERITNLVFMGMGEPL
                     DNLENVLSALKLIQDERALAFSPRRTTISTCGIVPGIYALADSGVKVKLAVSLNSARN
                     SIRDKLMPVNRKYPLSVLKKALLYFQKKNPFRITLEYILISGVNMEDEDLKALRQFSG
                     DLSCKINFIPFNTLPFLPYLSPGEEELKSFLLKARGALPQTITLRRSRGIDIQGACGQ
                     LVVESNKKSLRGAV"
     gene            complement(5134..6009)
                     /gene="ricT"
                     /locus_tag="PK015_05060"
     CDS             complement(5134..6009)
                     /gene="ricT"
                     /locus_tag="PK015_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOL33869.1"
                     /translation="MVEPLDLDEENSPEYLEVIFRTGRLGYYQNSSQLPLKPDDLIIV
                     EVDRGEDIAQVIHLSVSDEEVLAQIPQTPNPFKIKRIATEEDIEKLRHLPDEEKKAAQ
                     IFAEILKRYSFEMKLIDTVYQFDGNKITFFFTAEGRIDFRVFVRELANVFRTRIELHQ
                     TTGRDEARRMGGFGMCGLSYCCGNFLKHFNQVTIKMARDQNLAGNLAKISGPCGRLLC
                     CLNFEEDFYMEEGKLLPVPGTCVEYQGIKMYVLKTDPLKKNILLSDDDENITELDLET
                     FLTLKILSSPDLELC"
     gene            complement(6002..7207)
                     /locus_tag="PK015_05065"
     CDS             complement(6002..7207)
                     /locus_tag="PK015_05065"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HOL33870.1"
                     /translation="MIIFDDKKQNLFFPLTLNRSVGDLRCGILKLRQRLEELLDAEDT
                     TFWIDPDLQDLYKTRHPDWKINEPANQGTLMVNSRLKPSSESISAIKDLKPNQGLVQG
                     DDLIAFYAPKTISELPTALELKAAGIELIPSQLTLYQNLSELINDNERLIRLDFEQFF
                     YDQDNYFETEPGVTVLNPYYIWIGENVSLNPGCVLDASEGPIIIDEGTTVLPNAVICG
                     PAYIGKKSLIKVGAKIYEGTSIGPVCKVGGEVEESIIQAYSNKQHDGFLGHSYIGEWV
                     NIGAGSSNSDLKNTYKNVKFYSYASGEKIDSGTMFLGSMVGDYVKLGINTSVNTGCVI
                     GFGCNLWSSDLIADFIPDFSWGRASQLTKYRFEAFCETAKLVKKRRGLDLSPEEIALF
                     KKIWELNNG"
     gene            complement(7231..9291)
                     /locus_tag="PK015_05070"
     CDS             complement(7231..9291)
                     /locus_tag="PK015_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HOL33871.1"
                     /translation="MNEINKQKWNSVLKVIGIIFCICVGIFFGILWYYQDDLPPTSEL
                     KNYTLRTGSEVYDCNGKMVYLFAYERRKLVSLKELPQHLIDALLVTEDKRFYYHFGVD
                     PIRMVKALITDLKTGEFSQGASTITQQLARNMFLTLDKKLSRKLQEIVLAFKIESTFS
                     KDEILEIYLNKIFWGSQNHGIETASLYYFNKHARDLSLPEAALLVGMIQRPNYYDPFK
                     HPDRAKSRRDLVLERMYKAKKISKEEYLEAIATPLNPQSGGVYARFASDYFIEYIRIY
                     LENKYGTDRLFEGGLRIYTTLDADLSSAADSILNAYLRNVENSGGFRYKYSSVPKDAQ
                     DIDTKYIQGGLVLMENKTGYVRTLIGGRNFEHSKFNRMTQARRQPGSSIKPTYYTLAI
                     ERGYTPATILDDAPITIGNWSPSNADNKYHGPTRMRVALQWSYNAWAVRCAQDLGLST
                     LNEAFKRFGLNIYAQDMTASLGAYEVVPINLISAYTAFPNEGMRVKPVFITKVEDMKG
                     NVLERGSIQKYSVCSPQVAYLMTSMLQTVASSGTGAASRNGYFWDNAGKTGTTDANND
                     SWFIGFNKAYTLGIWMGFDNNKIKAGSAQAVRIWGPIMTQAIKNDNKGRTPKPNDSRY
                     SFTVPPRIVQLNINPYTGLVVSSGGIPEVFIEDNIPPTAPDSLRYGKTPGTPFFKEEL
                     EQEF"
     gene            complement(9284..9814)
                     /locus_tag="PK015_05075"
     CDS             complement(9284..9814)
                     /locus_tag="PK015_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HOL33872.1"
                     /translation="MKKQLALSSPVTLAFLTATACTIHIFESLIMRLLPLPFLRLGLS
                     NIIVMYLILKGKPLQAVLVNVTKSLVGGAVTFTLMTPATLLSLGGGLFAILAMWLACV
                     SNLGFTEIGISICGAIAHNIMQLFLVKTIVLPGTRVFVLTPLLLFLGLFTGIITAWLL
                     LLAEARFKIIKTDSNE"
     gene            complement(9816..10562)
                     /locus_tag="PK015_05080"
     CDS             complement(9816..10562)
                     /locus_tag="PK015_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOL33873.1"
                     /translation="MIEVKNLYLRLNGQLILEDIDFVLEDGHNMIILGRSGAGKTVLI
                     KTLMGFFSPDKGSVIVDGVDVYQDKDFHNSRLKHSFAMVFQNAALLDSFTVFQNVALS
                     LYERGEMDLSEIRERVRKSLALVGLESAENKYPSELSGGMLKRVGIARALAYEPRYII
                     FDEPISGLDPITASEILYYITQIIEQGEVTTITITHDISNIHQLGDRVLFLEQGKQLY
                     YGNLEGLYASQDPIIQKFLNLHNNKYNFNS"
     gene            complement(10534..10926)
                     /locus_tag="PK015_05085"
     CDS             complement(10534..10926)
                     /locus_tag="PK015_05085"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOL33874.1"
                     /translation="MGKQKSKRKSELKLWAPVGWLVIIWIVSSIPSQKFPTQEIFSFD
                     KVAHFSEYLLLALLVNSSFRERELRGRNIWWLYLILFIIAGLDEYHQRFIPGRTVCIE
                     DYLANASGLAVGLIFFWILYDRSKEPVS"
     gene            complement(10968..12245)
                     /locus_tag="PK015_05090"
     CDS             complement(10968..12245)
                     /locus_tag="PK015_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOL33875.1"
                     /translation="MIRQVDDGSALLIILLLLLLSAFFSGSETAFFSLPRLYLKKLEK
                     SKSSSAKRILKLLSKPRQLLITILLGNTFVNMAISSLGALLAIGIAQKHNFNTSTALT
                     IQVIITTIVIVIFGEIIPKLTALSTANKFAQITSLPLQFFSYLFYGPVWLIDKFNLLL
                     SKKKTVDRHMAEKVTTEEIHNLIQSESSKHTLDEHEKKMLVGLFRFKDAKISEIYVPR
                     VKVKAIEENSSLEELKELIVTTGYSRIPVYRKTIDEIVGIIYVKDLILYPEKKTIKEL
                     MRPAWFVTENMKVQTLLNQFKQKKMQMAIVVDEYGGTSGIISLEDILEEIVGEIQDEY
                     DQYEIPELSQIDENTYRMQGTVSIRLFNEELDASLDAEEYDNIAEFLLTQFNHVPAPG
                     ETFVWEDSLQFKVLDSNDKSIKLVEVTKLSQES"
     gene            complement(12226..12687)
                     /gene="ybeY"
                     /locus_tag="PK015_05095"
     CDS             complement(12226..12687)
                     /gene="ybeY"
                     /locus_tag="PK015_05095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOL33876.1"
                     /translation="MNYIDFNLVNDTEYLVDEKLLLSCAEKVLEEELPGKRVELNLLL
                     CDDEVMQNYNRTFRGDNSVTDVLCFNYYDSILNTENEAFPSLYCDIIIDIKQLERQKG
                     NKSMEDELIEVFIHGLLHALGYDHIRLEDRTVMEEKEKYYINKIVGDKTSG"
     gene            complement(12684..14801)
                     /locus_tag="PK015_05100"
     CDS             complement(12684..14801)
                     /locus_tag="PK015_05100"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOL33877.1"
                     /translation="METKYILIAIITALCIIGLFQLANEGDEGYPEYHLSVGKIADFE
                     LVAPFDFQILKSDEQLQEEYAQALRKADKPYTFSPDIEFEAYRNLDQLFNYIWEAREN
                     PNPESVILKAREEGFQLENIYSLLHANEDMINSLYNSVRNILTELYKVGIYQGITGDS
                     VLVVTPTGNKKFPRTRYFEVEEAKRLMKSRMGPMAQNLLLENNLNKLVMPNLVVDAVQ
                     YAKLQEEIKKQINPVAGIVLQNEIIIRKNQRITEEDINKLNSLVREYKARGERRTSLM
                     KWLSFMGLFIYILACLFAFNLYYWHTPYRETYGNNGTYILNLGFVLSVLMTIITCKML
                     NLNASLIPLVMFSVTVAIIIGFDFAIIYSIFSILMIAPFANWDAFNLPLLLTSILLLL
                     SLMRNFKSRHDFIRIGVYFFFILNILSYALGMVSYSGNDPGEKFQSLLQNTGYSLISI
                     IIAILGAMGLVRYFERKLHRATKQVLLELQDSNHPLLKRLATNAAGTYHHSLIVGNLA
                     EQAAAAIGANPLLARVGSYYHDIGKSVHPELFTENNEDSSEFHDKYTPEESADIIRDH
                     VKEGVVLAQKYHLPQPVIDIINQHHGTSYIRFFLDAAQRKGEVTDLSAFRYPGPLPQT
                     KEAALVMLADIVESTIKSKKDVTEEEIEKILDDTIQRLIREGQFNEAPITLKDLAKAK
                     EVMLPILAGIYRKRIEYPEEASP"
     gene            complement(14832..16211)
                     /gene="radA"
                     /locus_tag="PK015_05105"
     CDS             complement(14832..16211)
                     /gene="radA"
                     /locus_tag="PK015_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOL33878.1"
                     /translation="MIEENIDKEVEKMASQFFCTDCGYETNKWSGKCPACGSWGTIIE
                     SSRITGKNQSGKIPDLHQAKPECIKDIKTTEISRWKTGTAEFDLVLGGGIVPGMVVLI
                     GGEPGVGKSTLMLQLSEWMGKQGKKVLYATGEESAEQIQLRSTRLNISSENIWLLCTN
                     DAEHILNEVAENHSELLIIDSIQSVSLPNLDSLPGTVTQLRETCNRIIRCAKTMHLPV
                     FLVGHVTKEGFVAGPKIIEHMVDTVLYFEGELQGQYKILRAVKNRFGPTNEIGIFEMS
                     NIGLVEVPDPNQIFLSESGKHNGTAIGCVMEGSRSFIVEVQALATNSNYGTPQRVVSG
                     LEQKKLAILLAILEKNLALYLRGSDVFINLAGGIRSTDPGLDLAILSAIISSLKDKPV
                     PERSVFIGEVALNGEIRPVSQIEPRINEALKLGYERVIISGHSKVRQKAKVLKLFNVR
                     ELYSFLDKT"
     gene            16351..16620
                     /locus_tag="PK015_05110"
     CDS             16351..16620
                     /locus_tag="PK015_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HOL33879.1"
                     /translation="MKNNIIVFGTPTCPWCKKTKDYLTCNGFKFKYIDVSSNSKALQD
                     MERKSGHTGVPQLWINNKVVVGFDREKIDRLLNIKNKENNHESNA"
     gene            16604..17062
                     /locus_tag="PK015_05115"
     CDS             16604..17062
                     /locus_tag="PK015_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HOL33880.1"
                     /translation="MKVTPEDFHNLITRLQIVLSEIDYAQKACLQAGKMECQLLNYLY
                     NVQKPVNMNELAKELNVSHSRITRIMDNLVNKNLVIRKPSDEDRRCWYAIITDKGKKL
                     AENSRQTVLDHQKKIMSMLPEKEVEDIFSALKVYIDKYEEVLKNTKMEME"
     gene            17059..17487
                     /locus_tag="PK015_05120"
     CDS             17059..17487
                     /locus_tag="PK015_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOL33881.1"
                     /translation="MTHSTSTHWLGDMAFEANVNGHKIIMDTDAQGGGKDLGPRPKPL
                     LLAALSGCAGMEIVYLLKKMKIENYNLSIEAEADPVSEHPAVYKQIRLDFHFGGDNLP
                     QDKIAQAVNLSFDYCPVYAMLSKATEIISRVFINKIEVKL"
     gene            17586..18026
                     /locus_tag="PK015_05125"
     CDS             17586..18026
                     /locus_tag="PK015_05125"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOL33882.1"
                     /translation="MKAEAPDSLKTAQAAMDKYEPGTWLTDYNLALRLSKESGKPVLI
                     NFTGSDWCPWCFRLRDEVFSQDAFINFAKENLILLMIDFPMRKELPPEQVKANQALQE
                     KYGIEGYPTVVIVNAEGKEIARTGYKEGGAESYVAHLKDLLAKK"
BASE COUNT         5683 a         3629 c         3357 g         5760 t
ORIGIN      
        1 gataaatcaa accagcgctg gagtttttgt tgatagagca tattcaagct ttccagttct
       61 tcacttatac gaaggtaatc aagatgagta agagtaggag tagagttaag ggaatcttcc
      121 agatttttaa tctgagtctc caggttttct atctcttggg taagtaattc caattctcgc
      181 tgttctttat agcttatgcc cctggattga cgctgagatt taggggcaat attaagagta
      241 ggttttttat tttctttttc ttccttttga taattacgaa ccaataagta gtcagaataa
      301 ctaccaggga acttccttat tccatctttt tcaaagacaa aaagttgttc cacacatcgg
      361 tcaagaaaga aacggtcatg agaaacaacc agcagacatc ccttaaaatc atccagataa
      421 tcttccagta tttccagggt ttgaatatcc aggtcattgg taggttcatc cagaataata
      481 aaatttgcac caaacataag tgatttcaga agaaagaggc gacgcagttc tcctccagaa
      541 agagaagcaa gtttttgttg ttgcatctta tcatcaaaga gaaagcgttt tagcatctct
      601 ttagcggtca atttcaatcc tgactgagtc tgaataacct cagaatattg tgtcagatat
      661 tcaaagacgg aaagatctgg attgtagttt tcttcttcct gactgaaaaa ggaaaaccga
      721 gtattaactc ctactttgac ttttcctgta tcgggcttta tctcacctgc tataatcttt
      781 agcagagtag tttttccaca gccattgggt ccaataatac cgattttttc acctgcttga
      841 aagatatgct ccaagttttg gaataatagt tttccttcaa agcttttact gatattgtgg
      901 agttccagga tagttttccc ctgacgctcc ataagaaaag agattttgag ttctggtttt
      961 gaaatgagat aggattgaga gatgagtttt tgcactctat tgatatggtc tttaggtttt
     1021 gaagtccgag ctttagcacc tctttttaac caatcaagtt ctttctttag ctgtgcttga
     1081 cgccggattt cttttctctt ttggtcggtt agacggataa gtttttgttc cacataatct
     1141 gtataattcc cttcggtaat gtagcaatgg ctgtgttcta tttccagaat gcgattggag
     1201 acagcatcca gaaaatagcg gtcatgggaa acaaagagaa gtgtctttct ggatttagta
     1261 agataatcct gaagccattc aatcgtatcc aagtcaagat gattggtcgg ttcatccagt
     1321 aaaatgagat ctggttcacg agctaaaact ttagccagac ctgcttttct tctttctcca
     1381 ccggaaagta gactaagagg tttattccaa tcggagaggt taagtaagga aagcatagcc
     1441 ttatagcgat gttcttcagg aacagaggtt ctggataaat tctgatttaa aacatattga
     1501 aggatggtct cttccgcagg taagggttca ttctggggaa gccatccgag agttaagtct
     1561 cttcttttaa caatcgttcc tgtagtaggt tcaagattcc ccgccagtaa tcttagaaga
     1621 gttgattttc cggaaccatt tatccccaca atcccaattt tatcaccatt atgcactccg
     1681 agatttagtt cagcaaatat gactttactt cccagctgga gggaaagatt ttctacggtg
     1741 agaataactt cagagggcat aaatggttat atggcgtccc tgaggcgatt cgaacgcctg
     1801 accttcacct tcggagggtg ccactctatc cagctgagct acagggacgc aggatagtag
     1861 acccatagat taatttaatc aggtttttgt caatcccttt cccgtggatt gaactaagct
     1921 gtccagttca cttttcccgc atagataaga ggtggcaaaa ggagctaaag gacgaatata
     1981 ttcagcaaaa ctacaagcaa gattacggat ttcccattga gcatttatat cagcacgaag
     2041 tcggataaaa tgatagattt cacgaagatt catttttacc agaactcgca cttgttcagc
     2101 attcagacga gcataaggaa ccaggtcagg agagacttct gtcagttttt gttttaaaag
     2161 attcgtttcc ttcaaaagct ttaaccattt atcttctctt ttcagagtat agataatcgg
     2221 agggaagata taattattta gagagtaggc tttttgttta agaagagtcg ccaccctatg
     2281 ccttttaaat tgagaccaac aacattcaga catagcaagt tcaaagctac aatctaccag
     2341 ttcaaaagca cgaggcattt tgctccaagg ctggatatgc tcaaaaatct ctgcccataa
     2401 ctcttttttc ttagtttcat ccagtttagt tatagtttcc agacaggaag aaaaagaact
     2461 ggaacattgc tcaaagagga gagcggctaa gattttatca tcagcatcta cggaaaattc
     2521 cagtatttta actggggatt tatcaaatga attttcagta ataccagttt taatctttgc
     2581 cacatcaaaa gtgcctttaa aatcttcttt ttcaatatag cgaatgagag agggacataa
     2641 ttcttttact gattgcagca gaaggttata cagttcgtga gcttccgcta aaggagaagt
     2701 aatcaacctc cttaataaat tttccagact gcgggcattg atagtcattc ccaattgagt
     2761 tttggtggca agaggtaaga tataacgagc atcttcttta gctttgcctt caatttcaga
     2821 cttctttaac tgaggttgaa cctttttata atattcctgt atagctttaa ggctttcttt
     2881 atattcatta aaaagagctt gcataatctg agtatattct tcacgaagca gtttcagtgc
     2941 tggttgttcc agttcttcag ggataacaaa gtcgtcagaa aaagctacat aacgctggga
     3001 cttttcggta aaggaagcaa atcggatagt ttccactaat tcagttacaa gacgagaaat
     3061 gccaatcagg tcaaagttaa acacagaatg ttcagcaacg gaagaatgac caagttcaaa
     3121 gataattttt tgattggagc gccgagcttt ctccagctct tttagtgcct gttcccgaag
     3181 ttcagcaatg gatttttcac tacgcgagat tcgtgcataa gcagcagaga ttacttctgg
     3241 tgttgcactc tcagtttgta gcttacgaat taaagagcta tcaatattat agccagcgag
     3301 attgacctgc atagtaaatc taattaaagc aatgccttca cattgcttgc accgatgcga
     3361 ttagcacctg cattcaacat tgccagtgct tgttctctgg ttctaattcc tcctgctgct
     3421 tttactccca tcttaggacc aactatttct ctcaaaagac gcacattctc tacggttgct
     3481 cctgctgttc caaatccggt ggaagtctta acgaaatctg ctcctgcttt ctttgcgtaa
     3541 agagcacagg tgataatctc ttccttagaa agataacaag tctccagaat aactttaagc
     3601 aggacatcat tagaatgagt catttcagcg atatttttga ttaactgata ggaatgaaga
     3661 ggatgaccgc tcttaactgc actcaggttt tgcaccatat ccagttcttc aatgcccgta
     3721 ttaatgacag caagagcttc tgcagcaaca gcatagatat aacttgcacc cagcggaaaa
     3781 ttgataacgg tgcaggattt cacagaagca ttaagtctaa gttgcagagg atgagagaag
     3841 tgggaattta cgcatactgc agcacattta tattcattag caaggtcaca aagagcatta
     3901 acttcactct gcgtggcatc tgctttcagt aatgtggcat caataacgga ggcaatatca
     3961 gctaaggggt cataaagttg gtcaggttga tctgcacgac agatattaca cccgagacag
     4021 atattatgtc ctccaccaca tttacaagga ggattatctc caaaaagttt acgagcaata
     4081 tattctattt cattcataca gcacctctca aagatttttt atttgattcc acaactaact
     4141 gtccacaagc accctgaatg tctatcccac ggcttcgtct tagagttata gtttgaggca
     4201 aagctccacg agcctttagg agaaaggatt ttagctcttc ttcgccagga gagaggtaag
     4261 ggagaaatgg taaggtattg aagggtatga aattaatctt acaggaaagg tctccgctaa
     4321 actgacgcaa ggctttcaaa tcttcatctt ccatattaac tccggagata aggatatatt
     4381 caagggtaat acgaaaaggg ttcttcttct gaaaatagag taaagctttt tttagaacgg
     4441 aaagagggta tttacgattg accggcataa gtttatcacg gatagaatta cgagcagaat
     4501 taagagatac cgcaagtttc actttcacac cagaatcagc taaagcatag attccaggga
     4561 cgattccgca ggtggaaata gtcgttctgc gagggctaaa agcaagagct cgttcatcct
     4621 gaataagttt cagggcactg agaacatttt ccagattatc cagtggttca cccataccca
     4681 taaagaccag attggtaatc cgttcagtgg tctctcggga tgccagaaga aactgttgca
     4741 caatttcatg aggatacaga ttacggtgta gtttcatttt acctgttgca cagaaagaac
     4801 aatcacgagc acagccaact tgagaagaaa cgcaaagcgt aagttttttc tcttctggca
     4861 tcaagaccat ttcaatctgc tctccatctg ataataataa ccggaatttt tgactcccgt
     4921 ccatagattt aagagtttgt acgatttgtg gcatagagaa atcaaaggct tgtgataaat
     4981 agctttgcat agtagcgggc agatcggtca tcttagaagg gtcaaatacc tttttttcat
     5041 aaatccattt taacaactgc tgcgaacgat aagcaggata ttcagaggac aaaaaggcac
     5101 ttagctgttc aggcataatg ccataaatgc tatttaacat aattccaggt ctggagagga
     5161 aagaattttc agagttagga aagtctctaa atcaagctca gtaatatttt catcatcgtc
     5221 agaaaggagg atgttctttt tcagtggatc agttttcaag acatacattt ttatgccctg
     5281 atattccaca caggtgccag gaacagggag aagttttccc tcttccatat agaaatcttc
     5341 ttcaaaattg agacaacaga gcaatctgcc acagggacca gatatttttg cgagatttcc
     5401 tgccagattc tggtctcttg ccatcttaat cgtcacttga ttaaaatgct taaggaaatt
     5461 tccacaacaa taagataatc cgcacatccc gaaaccaccc attctgcgag cttcatctct
     5521 tcccgtggtc tgatgtagtt ctattctggt tctgaagaca ttagcaagtt cacgaacaaa
     5581 aacgcgaaaa tcaattcttc cttcagccgt aaagaagaag gtaattttat tgccgtcaaa
     5641 ttgatagaca gtatcaatta gcttcatttc aaaggaatat cttttcagga tttcggcaaa
     5701 aatttgagca gctttttttt cttcatctgg tagatgacgc agtttttcaa tatcttcctc
     5761 tgtggctatt cgttttatct tgaaaggatt aggagtttga ggaatctgcg ccagcacctc
     5821 ttcatcgcta acactaagat gaatgacctg tgctatgtcc tcacctctat ctacttcaac
     5881 aataatcaaa tcatcaggtt ttaagggaag ttgagaagag ttttgataat aacccagacg
     5941 tccggtgcga aatattactt ccagatattc agggctgttt tcttcatcta aatctaatgg
     6001 ttcaaccatt gtttaactcc catatctttt taaaaagtgc aatctcctct ggagacaggt
     6061 caagtccacg acgttttttt actagtttag cagtttcaca gaaagcctca aaacgatatt
     6121 tggtaagttg agaagctctt ccccaggaaa aatctggaat aaaatctgct atcaaatcag
     6181 aactccagag attgcaccca aaacctatca cacaacctgt attaacactt gtattaatac
     6241 ccaattttac ataatctccc accatagaac ccaaaaacat agtgccagaa tctatctttt
     6301 cgccagaggc ataggaatag aatttaacat ttttataggt attttttaaa tcgctattag
     6361 atgaaccagc accgatattg acccattcac ctatatagct atgtcccaga aaaccatcat
     6421 gttgcttatt ggaataagcc tgtataatgc tttcttcaac ctcacctcca actttacaga
     6481 caggtcctat ggatgttcct tcataaatct ttgctcccac tttgattaaa gattttttac
     6541 cgatataagc aggaccacag ataactgcat tgggaaggac agttgtgcct tcatctataa
     6601 ttatgggacc ttctgaagca tcaaggacac aaccagggtt caaggagaca ttttctccta
     6661 tccagatata ataaggattg agaactgtta ctcctggttc agtttcaaag taattatcct
     6721 ggtcataaaa gaattgttca aaatcaagac gaatcaatct ttcattatca ttaatgagtt
     6781 cactaaggtt ttgatagagg gtcaattgag aagggatgag ttctattcct gcagctttta
     6841 attcaagagc agtaggcagt tcagaaattg ttttaggagc atagaatgca attaaatcat
     6901 ctccttggac cagaccttga ttcggtttta agtcttttat agcactaata gattcagagc
     6961 ttggtttcaa acgcgagttt accatcagag ttccttgatt agcaggttca ttaattttcc
     7021 aatcaggatg tctggtttta taaaggtctt gtaaatcagg gtctatccag aaagtagtat
     7081 cctctgcgtc taaaagttct tccaatcttt gtctaagttt taagattcca caacgtaaat
     7141 cccccacact acggttgagg gttagtggaa agaataagtt ttgcttttta tcatcaaata
     7201 taatcattgt ttcctcggta ttgttagttc ttaaaattcc tgttccagtt cttccttaaa
     7261 aaaaggagtg cctggagttt tgccatatcg taaagaatcc ggagctgtgg gtggtatatt
     7321 atcttcaata aagacttcag gtataccacc actactgaca actaagcccg tgtaaggatt
     7381 tatattaagt tgaactattc tggggggaac agtaaaagaa tatcttgagt catttggttt
     7441 gggagtgcgt cctttattat cattcttgat agcttgagtc ataatgggtc cccaaattct
     7501 cactgcttgt gcagaacctg cttttatttt attattatca aatcccatcc agattcccag
     7561 agtataagcc ttattgaatc ctataaacca ggaatcatta ttggcatcag tagtccctgt
     7621 tttaccagca ttatcccaga aatagccatt acgggacgca gcaccagttc ctgatgaagc
     7681 tactgtttgg agcatactgg tcattaaata agctacctgg ggagaacata cagaatattt
     7741 ttggattgaa cctcgctcca aaacattccc tttcatatct tctactttcg tgataaagac
     7801 gggtttcact ctcattcctt cattgggaaa ggcagtatag gcagaaataa gattgatggg
     7861 tacaacttca tatgcaccta aggaagcagt catatcctgg gcgtatatat ttaaaccaaa
     7921 gcgtttaaag gcttcgttaa gagtgctcaa tccaagatct tgcgcacatc taactgccca
     7981 ggcattatag gaccactgta aggcaactcg cattcgggta ggtccatgat atttattatc
     8041 agcattagaa gggctccagt ttccgatagt tattggtgca tcatctaaaa tggtggctgg
     8101 agtgtaacct ctctctatag caagagtata ataagtaggt ttgatagaag aaccgggttg
     8161 ccgtcgtgct tgagtcatcc tattgaactt actatgttca aagtttcttc ctccaatcaa
     8221 agttcggaca tagcccgttt tattttccat tagaaccaag ccaccttgga tatatttagt
     8281 atcaatatcc tgagcatcct taggcacaga ggaatattta taacggaaac ctccactgtt
     8341 ctcaacattt ctaagatagg cattaagaat agaatctgcg gcagaagata ggtctgcatc
     8401 cagagtggta taaattctta aaccgccttc aaataaacgg tcagtgccat atttattttc
     8461 caagtagatg cgtatatatt caataaaata gtcggaagca aaacgggcat aaactcctcc
     8521 tgattgagga ttaagcggag tagcaattgc ctccagatat tcttctttac taatcttttt
     8581 agccttatac atcctttcca gcaccaggtc tcgtctgctt tttgctctat ccgggtgttt
     8641 aaagggatca tagtaattag gtctttgaat cattcctacc agaagagctg cctctggtag
     8701 gcttaaatcc cgtgcatgtt tattaaaata gtaaagtgaa gctgtttcaa taccgtgatt
     8761 ttgagagccc cagaagattt tattgagata aatctccaga atttcatctt tactaaaggt
     8821 agattcaatt ttaaaggcta acactatctc ctgtaatttt cgggaaagct ttttatccag
     8881 tgtaagaaac atatttctgg caagttgttg agtgatagtg cttgcaccct gagagaattc
     8941 tcctgttttc aaatctgtta ttaaggcttt aaccattctt atagggtcta cgccaaaatg
     9001 gtaatagaat cttttatctt cagtaaccag cagagcatct atcagatgct gaggtaactc
     9061 cttcaaagat accagtttcc gacgttcata ggcaaaaaga tagaccatct tcccattaca
     9121 atcatacact tcacttcccg tgcgaagagt gtaatttttt agctcgctgg ttggaggtaa
     9181 atcatcttga taataccata ggataccaaa aaatattccc acgcatatac agaagattat
     9241 cccgataact ttgagcaccg agttccactt ttgcttattt atttcattca ttactatccg
     9301 tcttaattat tttaaatctg gcttcggcaa gcaacaaaag ccatgcagtt attataccag
     9361 taaacaaccc taaaaataac aataatggtg tcaatacaaa aactcttgtt cctggtagca
     9421 ctattgtttt caccaggaaa agttgcataa tgttatgtgc aatagcccca caaatgctaa
     9481 tacctatctc tgtaaaacca agattggaaa cacaagccaa ccacattgca agaatggcaa
     9541 ataaaccacc acccagagat aatagtgtgg caggagtcat taaagtaaaa gttactgcac
     9601 caccaactag ggattttgtc acattaacca ataccgcttg aagaggtttt ccctttaaaa
     9661 ttaaatacat tacaatgata ttagataatc cgagtctaag aaaaggaagt ggcaaaagac
     9721 gcataattaa gctttcaaaa atatggatgg tacaagctgt agccgtaaga aaagctaaag
     9781 ttacaggaga agacagagct aattgcttct tcactttatg aattgaaatt atacttattg
     9841 ttatgaaggt tcaggaattt ctgaatgata gggtcttgac tggcatacag accttccaga
     9901 ttgccatagt aaagttgttt tccttgttcc agaaataata ctcggtctcc taactgatga
     9961 atattactaa tatcatgagt tatagtgata gtggtaactt cgccttgctc aattatctga
    10021 gttatatagt ataagatttc agaagcagtt atagggtcca gtcctgatat aggttcatca
    10081 aaaatgatat atcgtggttc atatgccaga gcacgagcaa tgcccaccct ttttaacata
    10141 ccaccactaa gttcagaagg gtatttattt tcagcagatt ccaaacctac caatgctaag
    10201 cttttcctaa ccctttctct tatttcagat aaatccattt cgccccgttc atagagtgaa
    10261 agagccacat tctggaatac ggtgaaagaa tccagcaatg ctgcattctg aaaaaccata
    10321 gcaaaactgt gcttcaatct ggaattatga aaatctttat cctgataaac atccacccca
    10381 tctacaatga ccgagccttt atctggagag aaaaaaccca ttaaagtctt aattaataca
    10441 gtcttccctg caccacttct tcctaagata atcatattat gaccatcttc caggacaaaa
    10501 tcaatgtcct ccaagataag ctgaccattt aatctaagat acaggttctt tacttctatc
    10561 ataaagtatc cagaaaaata tcaaacctac agccaacccc gaagcattag caagataatc
    10621 ttctatacag acagtcctgc ccggtataaa acgctgatga tattcatcca atcctgcgat
    10681 aataaatagg attaagtaaa gccaccagat atttcttcct ctcagttccc tttctctaaa
    10741 actggaattt actaataaag caagcaacaa atactcgcta aaatgggcaa ctttatcaaa
    10801 gctgaagatt tcctgagtag gaaatttttg cgaaggaata gaagatacaa tccagataat
    10861 aactaaccat ccgactggtg cccataattt taattctgat ttccgctttg atttttgttt
    10921 tcccatagtt tttctatccg ttaatccaga atttatcctc tcctgattta tgattcctgt
    10981 gaaagtttgg tcacctctac taatttgatg cttttatcat tgctatccag caccttgaac
    11041 tgcaaagaat cttcccagac aaaggtctct ccaggtgcag gaacatgatt gaattgggtg
    11101 agtaaaaact ctgcaatatt atcatactct tctgcgtcta aagaagcatc cagttcttca
    11161 ttgaaaaggc gaatactgac agttccttgc atgcgataag tgttttcgtc aatttgactt
    11221 agttccggaa tttcatattg gtcatattcg tcctgtattt ctcctacaat ttcttccagt
    11281 atatcttcca gagatatgat tccagaggtt cctccatatt catccaccac tattgccatc
    11341 tgcattttct tttgtttgaa ttgattaagc agtgtctgca ccttcatatt ttctgtcaca
    11401 aaccaggcag gtctcatcaa ttcttttata gttttcttct ccggatagag aatcaagtct
    11461 ttcacatata ttattccgac aatttcatct atggtctttc ggtacacggg aatgcgagaa
    11521 tatccagtag ttacaatgag ttcttttaat tcttccagac tgctattttc ctctatggct
    11581 ttaactttca ctcgtggaac atagatttcg gatattttag catctttaaa gcgaaacaaa
    11641 cctaccagca tcttcttttc atgttcatcc agggtatgtt tagaactttc agattggata
    11701 aggttatgaa tctcttctgt agttaccttt tctgccatat gtctatcaac cgtttttttc
    11761 ttggaaagca ataaattgaa tttatcaatt aaccaaacgg gaccgtaaaa aagatagcta
    11821 aagaattgaa gaggcaaact ggttatttga gcaaatttgt tcgcagtgga aagagctgta
    11881 agcttaggaa taatctcacc gaaaatgaca atcactatgg ttgtgataat gacttgtatg
    11941 gtcaaagcgg ttgaagtgtt aaaattatgt ttttgggcaa tcccaatagc cagtaaggct
    12001 cctaatgagg atatcgccat attaacaaag gtattgccta agagaatagt aattaaaagt
    12061 tggcggggct tactcaataa tttaagaatt ctcttagcac ttgagctctt acttttctct
    12121 aacttcttta gatagagacg aggtagggaa aagaaagcgg tttcagaacc agaaaagaat
    12181 gccgataata ataatagcaa aagtattatt aataacgctg aaccatcatc cacttgtctt
    12241 atcacctact atcttattaa tataatattt ctctttttct tccatcaccg tgcgatcttc
    12301 taatctaata tggtcataac ccagagcatg taataatcca tgaataaaaa cttcaattag
    12361 ttcatcttcc atacttttat ttcccttttg ccgttctaac tgttttatgt caatgataat
    12421 atcgcagtaa aggctgggaa aagcttcatt ttccgtattc agaatagaat cataataatt
    12481 aaagcatagc acatcagtta cagagttatc acctctaaag gttctattat agttttgcat
    12541 aacctcatca tcacatagaa gtaaattcaa ttctactctc tttccaggta attcttcttc
    12601 caagactttc tcagcacagg ataggagaag tttttcatcc actaaatatt ctgtatcatt
    12661 gactaaatta aagtctatgt agttcaagga gaagcctcct ctgggtattc aatccttttc
    12721 cgatagattc cagctaagat gggcagcata acttcttttg ccttagccag gtccttcaga
    12781 gtaatcggag cttcgttgaa ttgtccctct ctgataagtc tttgaatagt gtcatccagt
    12841 atcttttcta tctcttcttc agtgacatct ttttttgatt ttatggtaga ttccactata
    12901 tctgccagca tcaccagagc tgcttctttg gtttgaggta aaggtccagg ataacggaaa
    12961 gcggataaat ctgtgacctc accttttcgt tgggcagcat ccaagaaaaa ccgaatataa
    13021 ctggtcccat gatgttgatt aataatatca attacaggtt gaggaagatg atatttttgg
    13081 gctaaaacca ctccttcttt aacatggtct ctaatgatat cagcactttc ttcgggagta
    13141 tatttatcat ggaactcaga agaatcttca ttattttctg taaataattc cggatgtaca
    13201 gatttaccaa tatcgtgata atagctacca acccttgcta aaagaggatt agctcctatg
    13261 gcagcagctg cttgctcagc aagatttccc actatcaagc tatgatgata agttcccgca
    13321 gcattggtgg caagtctttt taaaagaggg tgattagaat cctgcaattc aagaagaacc
    13381 tgcttcgtag cacgatgaag ttttctttca aaataacgaa ctaagcccat cgctcctaaa
    13441 attgcaataa tgatagaaat caatgaatag cccgtattct gcagtagaga ttgaaacttt
    13501 tcaccaggat cattcccaga ataggagacc attcccagag cataagaaag tatattcaag
    13561 atgaaaaaga aatacactcc aatacgaata aaatcatgac gggacttgaa atttctcatt
    13621 aaggataaaa gcaataaaat ggatgttaat agcagaggca aattaaatgc atcccaattt
    13681 gcaaagggag caatcattaa gatgctaaag attgaatata ttatggcaaa atcaaaacca
    13741 attataatag ccacagtgac actaaacatt accagaggta tgagacttgc atttaaattt
    13801 agcattttac aggtaataat tgtcatcaaa acggaaagaa caaatcccag atttaaaatg
    13861 taggtaccat tattaccgta ggtttctcta tagggagtat gccaataata caaattgaag
    13921 gcgaagaggc aggctaagat atatataaat agtcccataa agcttagcca tttcattaag
    13981 gaggttctcc gttcgcctcg tgctttatat tcacggacta aagaatttaa tttgtttata
    14041 tcctcttccg taatgcgttg attcttccta attattattt cattctgcag aactatacct
    14101 gctacgggat taatctgttt ttttatctct tcttgtagtt tggcatactg aactgcatcc
    14161 accaccagat taggcataac caatttatta agattattct ccagtagtag attttgagcc
    14221 attggaccca tcctggattt cattaatctt tttgcttctt ccacttcaaa atatctggtt
    14281 cggggaaatt ttttattacc agttggggtt accaccagaa cactatctcc agtaatacct
    14341 tgataaattc ccaccttata aagctcagtt aagatatttc ttacactgtt atataaagaa
    14401 ttaatcatat cttcattagc gtgtagcaaa gaatagatat tttctaattg aaaaccttcc
    14461 tctcttgctt ttagtatcac cgattcagga ttaggatttt ctcgtgcttc ccagatataa
    14521 ttaaataatt ggtcaagatt gcgataagct tcaaactcaa tatcagggct gaaagtgtag
    14581 ggtttatccg ctttcctaag agcttgagca tattcttcct gcaattgctc atcactcttt
    14641 aaaatctgaa agtcaaatgg agctactaat tcaaaatctg caattttgcc gacgcttaga
    14701 tgatattccg gatacccttc atcaccttca ttagctagct gaaatagtcc tataatgcaa
    14761 agggcagtaa ttatagctat taatatatat ttagtttcca taagtttaaa tttatataat
    14821 cggttataat gtcaagtttt gtccagaaac gaatatagct ctcgcacatt gaaaagcttt
    14881 aatacttttg ctttttgacg cactttggaa tgtcctgaaa taataactct ttcatagcca
    14941 agttttagag cttcattaat gcgaggttct atttgtgata cgggcctaat ttcaccattt
    15001 aatgccactt ctccaataaa aacagacctt tcgggcactg gcttatcttt taaactggaa
    15061 ataatggcag acagaatggc aaggtctaaa ccaggatcgg tggaacgaat tcctcctgca
    15121 agattgataa atacatcact gccacgaaga tagagtgcta aattcttctc cagaattgcc
    15181 agtaatattg ccagcttttt ctgttccaat ccagatacca ctctttgcgg tgtcccatag
    15241 ttagaatttg ttgccagagc ttgaacttct acaataaagg aacgagaacc ttccataacg
    15301 cagcctatgg ctgtaccatt atgctttcct gattcggaaa gaaaaatctg attggggtca
    15361 ggaacttcta ccagtcctat attgctcatc tcaaatatac caatctcatt ggtcggtcca
    15421 aacctatttt tcactgctct taatatctta tattgacctt ggagctctcc ttcaaaatat
    15481 aaaactgtat ctaccatatg ttctataatt ttaggaccgg caacaaaccc ttctttagtc
    15541 acatgcccta ctaaaaacac cggcagatgc attgtctttg cacatcttat aatccgattg
    15601 caggtttctc gtagttgagt aaccgtacca ggaagagaat ccagattggg caaactgact
    15661 gattgaatgg aatcaatgat aagaagttca gaatgatttt ctgctacttc attcagtata
    15721 tgttctgcat cattggtgca aagtaaccag atattttcac ttgaaatatt taaccgtgta
    15781 ctgcgtaatt gtatctgttc ggcactttct tctccagtgg catagagcac ttttttgcct
    15841 tgtttaccca tccattccga tagctgtaac atcaaagtag atttccctac tccaggttca
    15901 ccacctatta aaacaaccat tccaggaact attccaccac ccaaaactaa atcaaattct
    15961 gctgttccgg ttttccaacg agaaatctct gtggttttta tatctttgat gcattccggt
    16021 tttgcttgat gtaaatcagg aattttacct gattgatttt ttccggttat gcgcgaactc
    16081 tctataattg tgccccaact tccacaagca ggacactttc cactccattt atttgtttca
    16141 tatccacaat cagtgcaaaa aaattgactt gccatctttt ctacctcttt atctatattt
    16201 tcttctatca aaaaaatgac aagttttttc ttgcttctaa ctcatagttt tcaagtttat
    16261 actttatctg acaaaaatgt atatgccctt gaaaattttc ttgctttttt tctggactgc
    16321 attattattg ctttaagagg ttcataaaag atgaaaaata atataattgt ttttggcact
    16381 ccaacttgtc cctggtgtaa aaaaacaaaa gattatctta cctgtaatgg ttttaagttt
    16441 aaatatattg atgttagttc aaatagtaaa gctttgcaag atatggaaag aaaatctgga
    16501 cacactggag taccacaact ctggataaat aataaagttg tagtcggttt tgaccgggaa
    16561 aaaattgacc ggctactcaa tataaaaaat aaggagaaca accatgaaag taacgcctga
    16621 ggattttcac aatctaatca ctcgtttaca aatcgtttta agcgagattg attatgcgca
    16681 aaaagcttgt ctgcaagcag ggaaaatgga gtgccagctg ttaaattatc tttataatgt
    16741 gcaaaaaccg gttaatatga atgagctggc taaggaactt aatgtctcac atagccgtat
    16801 aaccaggatt atggacaact tggtaaataa gaatttagtt atccgtaaac cttccgatga
    16861 ggaccgtcgt tgctggtatg ctattataac tgataagggt aaaaaattag ccgaaaatag
    16921 tcgtcagact gttcttgacc accagaaaaa aataatgagt atgcttcctg aaaaagaggt
    16981 ggaagatatt ttcagtgctt taaaagttta tattgataag tatgaagaag tattgaagaa
    17041 tactaaaatg gagatggaat gacccatagc acttccacac attggctggg agatatggct
    17101 tttgaggcaa atgtcaatgg ccataaaata attatggata ctgacgctca gggcggagga
    17161 aaggatttgg gaccgcgtcc caaacccttg cttcttgccg ctcttagtgg ctgtgccgga
    17221 atggaaatag tctatcttct gaaaaaaatg aaaatagaaa attataatct ctctattgaa
    17281 gctgaagcgg accccgtgtc tgaacatcca gctgtctaca aacaaattag attagatttc
    17341 cattttggcg gtgataattt acctcaagat aaaatagcac aagcagttaa tctatcattt
    17401 gactattgcc cagtctatgc tatgctctca aaagcaacag aaatcattag ccgtgttttt
    17461 atcaataaga tagaggttaa gctatgaaat acattatcat ccccgttata atagccgtct
    17521 gctctatttc tttcttaagc tgcagcacta agaacaatcc cgatcaatta aaccaggagg
    17581 aggtaatgaa agcagaagct cccgattctc taaaaactgc tcaagcagca atggacaaat
    17641 acgaacctgg aacctggctt accgattata atcttgctct ccgtttatct aaagaatctg
    17701 gtaaacctgt gctcattaat tttactggtt cagattggtg tccctggtgt tttcgtttgc
    17761 gtgacgaagt cttctctcaa gatgccttta ttaactttgc aaaagaaaac ctaatccttt
    17821 taatgattga tttccctatg cgtaaagaac tgcctccaga acaagttaaa gctaaccaag
    17881 ctttgcaaga aaaatatggg attgaaggct atcccactgt tgtcatagtt aatgcagaag
    17941 gaaaagaaat agcacgcact ggctataaag aaggcggtgc tgaaagttat gtggctcatt
    18001 tgaaggactt actggcaaag aaataaacag attattcatt agttagataa tattaacttc
    18061 ctgaaagagg aaaagagggg agatatttaa tcatttctgt aaggcttctg gagatggtgg
    18121 tgagatggtg agaatggtgg tgagtgttta tggtggtgag acgtcctcgt ctagtaatgg
    18181 tggtgagacg tccccgtctc gccaagccgt aaggcttagt aggggggtga gatgtcctca
    18241 tctcacaaag ccgtaaggct tagtaggggg tgtgagatgt cctcatctca caaagccgta
    18301 aggcttttat ataaaaacat ataagctctt atcattacca tataaagaat aagtataatt
    18361 ttcccttcaa ttaagaaagc tccttcggag cttcataaac gaggacgttt atgaccccgt
    18421 gtaagagga
//
LOCUS       DAORZF010000034        18393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_2501_length_18393_cov_372.342077, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000034 DAORZF010000000
VERSION     DAORZF010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 18393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18393
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2501_length_18393_cov_372.342077"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /gene="tilS"
                     /locus_tag="PK015_05130"
     CDS             <1..649
                     /gene="tilS"
                     /locus_tag="PK015_05130"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF023162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOL33883.1"
                     /translation="HFSKLEQYYIIREVIAEISGSRRDFFAHNFENIRNLFAAEGSKK
                     VSLNNGLEVVKEYDKLIIKFSEEPKPVPEPIVVEEDRSRVVWGDYRFSFKLLKVLPNR
                     FSNDPLTVCLDADKIKYPFVIRSRKPGDRFMPLGMNQIVKLKDFLINCKVPKSERDYI
                     PIFDDGEKIFWVTGKRLDQRVAIDDNSTRFLHITAEKIQDKQLRPANRSRTGEKK"
     gene            655..1191
                     /gene="hpt"
                     /locus_tag="PK015_05135"
     CDS             655..1191
                     /gene="hpt"
                     /locus_tag="PK015_05135"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOL33884.1"
                     /translation="MNCDLAALLFDEFRIQSRVREIGMQITQEYKDKVPILVGILKGG
                     FMFLADLCRSISIPVELDFLAISSYGKEASSSGVVKIRKDIDLDITGRDVIIVEDIVD
                     TGLSLQYIKEYLSKHSPATLKTCVLLDKPAAHKIDVSFDYVGFEIGNEFIVGYGLDYN
                     EQYRNLPYLGILKEELYK"
     gene            1188..3446
                     /gene="ftsH"
                     /locus_tag="PK015_05140"
     CDS             1188..3446
                     /gene="ftsH"
                     /locus_tag="PK015_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOL33885.1"
                     /translation="MKYISLILILLMSYAGLSAANTVNTPAQPMDQSSQEAVMQEFSQ
                     LLKWFVIALIIISVISLIRIFRLRHKMNAGKKQQPPQSRPYQTRRNVKPGAPKSQRQN
                     MPPGRPTQTPISFTFIIIMVMLGLMLLFSWLGGREQIIEASYTEFTAQLAQGEIKSVT
                     FTDQDMIYTTLSGKKYHTLLPPLEDPNLMPQLLENKVEINTKKPSHWASILSYLLPLV
                     LLLVFWWLLMRGVSNQNTQAFSFSKSKARLHQGDRTTVTFKDVAGVDEAKEELQEIVD
                     YLKEPRKFQRIGARIPRGVLLVGRPGTGKTLLARAVAGEAGVPFYSISGSDFVEMFVG
                     VGAARVRDLFDQAKKNAPCITFIDEIDAVGRHRGSGLGGGHDEREQTLNQLLVEMDGF
                     EINDSVIIIAATNRPDILDPALLRPGRFDRQVTVDLPDIKGRTEILKVHASKLPLAPD
                     VNLEVIARGTPGFSGAELANIVNEAALLAARQGKQLIDMEDFEEAKDKLILGKEKKSR
                     VIPEEDKKLTAVHEIGHVLTSVFQETTEPVHKVSIIPRGFSAGATHFLQTDRMNYSRK
                     YLEETMVELLGGRAAEEIVFGELTTGASNDLERVTEIAKKMVCVWGMSDAFGPMTIGK
                     DQTEVFLGKELVERDVHSNETAQLVDSEIRGFIYSAYDRALNILRRHINILEKMSAIL
                     LEKEILGTDEIFEFLLAEVEEDEKDIIMKKYQKAKELRFEHSVKPVPPENPETKEQQE
                     SAAQTPPSEPQD"
     gene            3521..4828
                     /locus_tag="PK015_05145"
     CDS             3521..4828
                     /locus_tag="PK015_05145"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOL33886.1"
                     /translation="MSKLKELFISLTVRNYRLFFMGQLISLIGTWVQRTAMSWYVYRL
                     THSPFLLGVVAFLSMIPALFISPFAGAWADRWDRRKTLIGTQILFMLQASLLAAGVLT
                     GFINELRWWPLLVLALFQGCVDGVDAPFRQNFVLDLVGKKSLLPNAIATNSAMFNSAR
                     LLGPSIGGIMIVLVGEGICFLINALSYLAVIGSLMAIRIQYRKPPESKENIFAKIKEG
                     WSYSWRNLPIRWLIANLGVFMLFAMSYTSVIPVFARDVLKGNAGTQGLLLTCAGVGAL
                     ASAIYMAGRKTIKGLPWVSIMMGCIGSLALIGFSQSTNLWLSMLMMVFVGMGLTIQMA
                     ITNTLLQTISYPSMRGRVLSVYTMTLNSLTPFGSLLLGWLTREIGPRYALTICAAICL
                     LWVLSGIKMVPKVSKNILRMLVKNKSTELYRPITVHPVYSETV"
     gene            4828..5517
                     /locus_tag="PK015_05150"
     CDS             4828..5517
                     /locus_tag="PK015_05150"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HOL33887.1"
                     /translation="MKTFVFFGDKQCDYRSLMANQKDFYVGDRLILASQTVIAEQVAS
                     DKVYLCSKKDNGAGFGSHSQVPGVDALITAIPNQYLLIRTADCFPILLEDKKQRVVAA
                     VHSGREGTRLNLVGKTINALKEKLEVMAQDLIAYIGAGICGEHYTVDEGTWRFYNECM
                     EQQGFFIGKVPFCHIDLRAGITQQLLAAGVPSNQIVHINTCTFESPLYFSYRRDKTDN
                     RQISLIGLIDE"
     gene            5510..6796
                     /locus_tag="PK015_05155"
     CDS             5510..6796
                     /locus_tag="PK015_05155"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HOL33888.1"
                     /translation="MSKILTNPDGNIKLELKDDKLSAWLTIKKDNKLIDEKDILDLLE
                     RAGIKNGFEEAQRYMRKHSMEKDFDFPFPIAMCSRVQGETKLNYYFNLNQAKQRQREI
                     HLDDLPNLTRIEEGTVIADYNNDIFDRQGSIYDIYGEMLPDEELDLQAAQQIAGENVT
                     FDINRQQYLAKCAGIPRVDEEGRISIIDKVILKEGISYPAEIIRAPISLEVVGDIIGA
                     QIAVAGKLIIQGDVVDSRISCQDNIQVQGDIKNCEIPGLEIKGDVHCKKIICSKVLCY
                     GELTFTELIKDSEIIANNGISGPSGWLQGGHTESGGNIELQNLGDPEGKLTEVEISIS
                     PWHKAALMQLTRKMIQLKKNPLTNAAEIEILNERIAACESELDNALNEFLQRPPEDKL
                     KIRVTQDVFPPVNLRILKNEYIIKDKKPSLEIIEID"
     gene            6807..9938
                     /locus_tag="PK015_05160"
     CDS             6807..9938
                     /locus_tag="PK015_05160"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOL33889.1"
                     /translation="MKRIMIIVCLGVMIISCTALIPDFTTDPAISPDGNQICFVYDGD
                     LWLVPFQGGSAKRLTATGAKEWGPAWSPDGKLIAFNSDRDGNVFPYIISATGGSAVPV
                     IEESYTVCDWFNNGQELLCTRSTLEFGTSFYRLSLSGKRPELIAEIGDPFASLSPDNS
                     CLVFNRYGYPYREAYKGSQAGELWKIDLSSKTYTRLTYTDYTERYPRFSYVNDYLYYC
                     ASDEERFQIYRVTSLDFSKVEQITNLTDWSARDISIARQNDRLVYEYFNEIWRWDPET
                     GIAAQVPILIDEDMWIPDLHQEVVYNNIEDFAISSDELLVGFTYKYDAFFMPRKGGKV
                     VQIDTGQTAIGDMEFLDDNRTLVLIRMDKGIDKLYKVRCDSLLQVTPIDWFGADSLDV
                     EGLYKDPSGKWVIFYGDKLMSGRIAVADSNLYNIRPINNSRAALNIFAINSSGDYGIY
                     ATTREDVWIRELWLYDFNTQESKKIMNDDAWTRSMLWTPDNKSILMSRSNGIFRLDLA
                     PRDEFEYEVDNWKEILNPQVTSKATKTVNQEENRDQDSTPSSTEKLEKQELNIVWEGI
                     EKRLYPILSESNRILSLGKVIDDSTFYYLSYSHDETASYQLKKANIYGKNSKTIQNLG
                     QNISNLRWVGNSLYFLENGKLKAYNLDKHSISEVTANYDYSFSESKLNLRVFEQVWGA
                     FGLNFYDPNMHGKNWEDLFKLYYPYAERARSISDIEQIVNEMIGDVNASHTGFYPRAQ
                     KVKPRPPRAYLGLEFDYSQILEEGIPIKVVYPQSRLADYYHIQNGDILLAIDGTPITP
                     YSSLDKLLAGKAGKLIKLLILKEGQKIEAELNGLTWSEQRKLYYDYKTERSKKMVKEL
                     TQDRIGYVHIPAMGNTDYENFRREVFRDNADKDALIIDVRGNSGGYIHDKLISMLNQR
                     YYAYSTSRRYSGKLNPEPRGIWAKPTILLVDEHSFSDGEIFPAVYQELKLGKIVGQPT
                     SGSVIGTWEFRLLDGSSMRLPGSGWYRLDGTNMEGNGVQPDIVVENKPEDIIAGKDAQ
                     LLRAIEEILKELN"
     gene            10046..10915
                     /locus_tag="PK015_05165"
     CDS             10046..10915
                     /locus_tag="PK015_05165"
                     /inference="COORDINATES: protein motif:HMM:NF024574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOL33890.1"
                     /translation="MAAEKELQNLLLRAEALMKTNWIYAVQLLNKAAEENPEDPRPLI
                     ALGDFYQQRQLFNKAVKYYQSALKLSPDDDRLKLIIGNTLFSEGEYQLAIVYYDEIED
                     QNVDVRYNKALALAYLGRNRESINIMRDLLDLIDNNPFIYFLLIEQLMHIEEYEEAQT
                     YIRKAEKRIGEHRHLILLKALIYAHFQNWLLAYNAFYNYEQSGDIVQSEHIYIYADCA
                     VKSGMSNRAIQILEKGLNDNPYSIPLYEELIRLLIQQKRIIKARYYMQEAKHHFSVLS
                     PLLQLMDARLNRM"
     gene            10925..12115
                     /gene="manA"
                     /locus_tag="PK015_05170"
     CDS             10925..12115
                     /gene="manA"
                     /locus_tag="PK015_05170"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HOL33891.1"
                     /translation="MPVFLLSNPLKHYEWGSPYYIPQLLKMEPYPDKPVAEMWIGAHP
                     ADSSILSEVEGQPSLREYILNHPVEALGKAKNLYNGELPFLLKVLAAEKALSIQVHPS
                     AEKAKEGFLQENKRGIPLDSPTRNYSDPHPKPELLCALTEFKALCGFRNPEQIVANFR
                     AVNLDKLFPSLSTLAQKQDEATLKNFTQEVLSVSGEKLNQTLRILEHSLNLNRNLPED
                     VVSAFNLIRKHFNEDAGLLAPLYLNTYTLNPGEALFLEPGVMHSYLGGAGIEIMANSD
                     NVLRAGLTPKYKDIPEVLNTLVYKPYVQKVILPKNIDDIYFVYSVPVKEFELQSLHIM
                     PGLNLKLQLHNMPVIILCYQGEVTILEHQKILSPGKATFITADIPELSLKGQGILWLA
                     TVPT"
     gene            complement(12116..13705)
                     /locus_tag="PK015_05175"
     CDS             complement(12116..13705)
                     /locus_tag="PK015_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33892.1"
                     /translation="MRKISLIFLLLIPFLLVAQSFNDEGMMDEETGREFTPSSPALGL
                     GGTLGNILIDGTYYTQFRFQPEITIWKFGLGLDIDLLIDSNGNVRKEDWDSWDDALSK
                     LYYFRFAQRQDPFYFKVGSINDYTMGHGLIFDEYSNMLHYPNVKPIGAYVGTNLKSLG
                     AGFEVFTHDVSKNEILGGHLYIQPLKPTGIPIVKNLKIGASIGMDRDPYGKYEDSDGD
                     GYPDVYDKFPNDPYYWLDTDNDGIPDNMDIDLNGNSILDHPSLNPYVNSEFPGIADNY
                     PNYPFDTQVYPDEAMPIDQSNEITIYGLDYELPILNKKSFGLSHYAEYAKIEDYGSGI
                     IFPGFAARFLIFNLNLEFRSFKDQFLPGFFNHLYDEQRSQVVYTTIESENGRRCYSLK
                     TKESELAGVTSSLGWFGSLKANLGNFAYLRVTYQDMYNDSKTKGKSLWGELTVIPNII
                     PKLQEASIVYGQSNVDYISFDTLRNENAEIGGKIVYNLSSSFNLALRYNEYYRDLNRD
                     GKIKGNDEIIESMSLGIEFQF"
     gene            complement(14615..16705)
                     /locus_tag="PK015_05180"
     CDS             complement(14615..16705)
                     /locus_tag="PK015_05180"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOL33893.1"
                     /translation="MPKTENPLLKERNEHRLQAIPFDEIKTEHFMPAIQEALKMAKAE
                     IESLKNNPAEPDFENTILALELSGEKLEYVASIYFNLLSAESDAEFKALAQQISPLLA
                     EFSSSIMTDPVIFSRVKKVWEKEVQNRPKPQIPKDFSEKQYLQQAERYRLIDRVYKGF
                     IRGGALLTPEEKEELTEIEMEASKLGPKFSDNVLAATNAFELYITNPSDVEGIPQNVL
                     ERAAHQAKVKGKKEGWLFTLQPSSMVPFLTYCKNRELRRQIQIAYNSRAFNDKFDNQE
                     IIKQLLKLRERRAKLLGYPTYADYVLEDRMAESVQNVINFLEKIYQVAYPAAQNEYKE
                     VVEFAREIDNITDFQPWDLAYYSNKLKEKKYAYDPEELRPWLKVEDVLSGLFIVANRL
                     YGIEMKQVNDIPTWHPEVSTWEAYDRDGNYLGLLYLDLFPRETKRGGAWQTTFQTAGL
                     YSDGIRRPQVAIVASLTPSTPEQPSLLLLDEARTIFHEFGHALHSLLADGYYKSLSGT
                     NVLWDFVELPSQLMENWLLEEEALKLFTRHYETGEPLPEELLQKVIAAKNFQAGLANI
                     NQLRYGYLDLAWHITPVEKISDVDAFEKEVTARFQVQPPVPKSNISCAFAHIFAGGYS
                     AGYYSYKWAESLEADVWSLFKEKGIFNPEVANAFRQYILARGNAFHPMDLFVAFRGRK
                     PDPEALLRRDGLLS"
     gene            17127..>18393
                     /locus_tag="PK015_05185"
     CDS             17127..>18393
                     /locus_tag="PK015_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33894.1"
                     /translation="MLKKLVFLIVAVLSFTYLIGLTAGDIAILGVHTDTPKSMIFVAL
                     ADIPANTQISFTDNGWNATTQQWRGNEGTIVWKHTELVSKGTCITLSINTSPYSATLG
                     TVTTHANFSLATAGDQVLAYEGTTPPTSNDASIWLYGISTSNWVWGNSSTTSDLPTAL
                     ANAAVAFTGTTTDIDNGYFANGSEPQTSVSVSGTKAQLLSLFGDRTKYYTNDNGPLTF
                     PTYTITVGSEPVISVSGTLNPFSTIVGTPSPAQNYTLIGQNLTANIIVTSPTGFEIST
                     DGSSYSSNLSLSPTYNGSIYVRLTGATIGTFSGNITHTSTGAAQVDLPVTGTVLETQP
                     PVLHITGTLTKFTMEQGTPSESQGYNLYGEYLTQNISIIPPAGYQLSTDNTNWSGQLS
                     LNPSFNGTVYVRLNGTNITSYNGNIVHTSG"
BASE COUNT         5896 a         3133 c         3585 g         5779 t
ORIGIN      
        1 acattttagt aaattagagc agtattatat tataagggaa gtaattgcag aaataagtgg
       61 aagtaggcgt gacttttttg ctcataattt tgaaaatatc aggaatttat ttgcagctga
      121 aggtagtaaa aaggtcagtt tgaataatgg attagaagta gttaaagaat atgacaaatt
      181 aatcataaag ttttcagaag agcccaaacc cgttcctgag ccaatagttg tggaagaaga
      241 tcgttcacga gtagtttggg gtgattatag atttagtttt aagttattga aagtgcttcc
      301 caatcgtttt agtaatgatc ctttgacagt atgccttgac gcagataaaa taaaatatcc
      361 ttttgtaatt cgtagtcgta aacctggtga tagatttatg ccattgggta tgaatcaaat
      421 agttaagtta aaagactttc taataaattg taaggtgcca aaatctgaac gggattacat
      481 tccaatcttt gatgatggag aaaaaatttt ctgggttacg ggtaaaagat tggatcagag
      541 agtggcaatt gatgataata gtactcgttt tttgcatata actgcagaaa agatacaaga
      601 taagcagctt agacctgcaa atcgtagcag aacaggagag aaaaaatgaa taaaatgaac
      661 tgtgatcttg cagctttgct ttttgatgaa tttcgcatcc agtccagggt gcgggaaatt
      721 ggaatgcaaa tcactcaaga atataaagat aaagtcccta ttctggtggg aatattaaaa
      781 ggcggattta tgtttcttgc cgatttatgc cgaagcatct ctattcctgt ggaattggat
      841 tttcttgcta tttcaagtta tgggaaagag gcttccagtt ctggagttgt aaagattcgg
      901 aaggatattg atcttgacat aacaggcaga gatgttataa ttgttgaaga tattgtagat
      961 actggtttgt ctttacaata tataaaagaa tatttatcta aacattcacc tgcaactttg
     1021 aaaacatgtg tattgttgga taagccagca gcgcataaaa tagatgtgag ttttgattat
     1081 gtaggatttg aaataggtaa tgaatttata gtaggttatg gattagatta taatgagcaa
     1141 taccggaatt taccttattt aggaatattg aaggaagaat tatataaatg aaatatatca
     1201 gtctaatact aatattatta atgagttatg caggtttaag tgctgcgaat acagttaaca
     1261 ctcctgctca acccatggac cagtcttctc aagaagcggt tatgcaagaa ttttcgcaac
     1321 tattgaaatg gtttgttatc gccttaataa tcatttcggt gattagttta atacgcatct
     1381 tccggttaag acataaaatg aatgctggaa agaagcaaca gccacctcaa agtagaccat
     1441 accaaactcg tcgtaatgtt aaacctgggg ctcctaaatc tcagcgtcaa aatatgcctc
     1501 cggggcgtcc gactcaaaca ccaatctctt ttacttttat aataattatg gtaatgctgg
     1561 ggttaatgct acttttcagt tggttgggag gacgggaaca aataattgaa gccagctaca
     1621 cggagttcac tgctcaatta gctcaaggcg aaatcaaatc tgttactttc accgatcagg
     1681 atatgattta tactactcta tcggggaaaa aatatcatac tttattacct cctttagaag
     1741 atccgaattt aatgcctcaa ttactggaaa acaaggttga gataaacacc aaaaaaccct
     1801 ctcattgggc aagcattcta tcctatttac ttccgctggt tcttcttctg gtattttggt
     1861 ggcttttgat gcggggagta agtaatcaaa atactcaggc atttagcttt agtaaaagta
     1921 aagcaagatt gcaccagggt gatcgaacta ccgtaacctt caaagatgtg gcaggagtag
     1981 atgaagcaaa agaagagttg caagagatag tggattatct gaaagaacca cgtaaattcc
     2041 agagaatagg agctcgtatt cctcgtggtg ttttactcgt aggacgtcca ggaacaggta
     2101 aaactctact tgcacgagca gttgcaggag aagctggggt tcccttttat agtatcagtg
     2161 gctctgactt tgtagaaatg tttgtgggag tcggtgcagc aagggtaaga gacctttttg
     2221 accaagccaa gaaaaatgct ccctgcatta cttttattga tgagatagat gcagttggaa
     2281 gacatcgtgg ttcaggtttg ggtggaggtc atgatgaacg cgagcaaacg cttaatcagc
     2341 ttcttgttga aatggatggt tttgaaatca atgattcagt aataataatt gccgctacta
     2401 atcgtccgga tattttagat cctgcattat tgcgtcctgg tcgttttgat cgtcaagtaa
     2461 cggttgattt accagatatt aagggacgaa ctgagatttt gaaagtgcat gcttccaaat
     2521 tgcctcttgc tcctgatgtg aatctggaag ttatcgctcg gggcactcct ggttttagtg
     2581 gtgcagagtt agctaatatc gttaatgaag cagcacttct ggcagcacgt cagggtaagc
     2641 aactaattga tatggaagat tttgaggaag caaaagataa gttaatttta ggaaaggaaa
     2701 agaagagcag ggtaatccca gaagaggata aaaaactcac agctgtgcat gaaataggtc
     2761 atgttttgac ctctgtattt caggaaacta cggaacctgt gcataaagta tccattattc
     2821 ccagaggttt ttctgctgga gctactcatt ttcttcaaac agatagaatg aactattccc
     2881 ggaagtatct ggaagagact atggtagaac ttttaggagg acgtgctgca gaagaaatag
     2941 tatttggaga attgacaacc ggtgccagta atgacctgga aagagttacc gagattgcca
     3001 aaaagatggt ctgtgtgtgg ggtatgagtg atgcctttgg acctatgaca attggcaaag
     3061 atcaaactga agtttttctg gggaaagaat tagtggaaag agatgttcat agcaatgaaa
     3121 ctgctcaatt agtagatagt gaaatcagag gctttattta tagtgcttat gatcgtgctc
     3181 tgaatatctt acggcgacat attaatatcc tggaaaaaat gtcagctatt ctattagaaa
     3241 aagaaattct gggcacagac gaaatatttg agtttctgtt agcagaagtg gaagaggatg
     3301 aaaaagatat cataatgaag aagtatcaaa aagccaaaga gcttcgtttt gaacatagtg
     3361 ttaaacctgt gccacctgaa aatccagaga ctaaggaaca acaagaatct gcggctcaaa
     3421 ctcctccttc ggagcctcaa gattgaatct taagagctat tatagctcct aatttataac
     3481 attcttggga gcataatggt taattatatc tgttaaatac atgagcaagc tgaaagaatt
     3541 gtttatctcc ctaacggtgc gtaattacag actgttcttt atgggacagc ttatttcttt
     3601 aattggtacc tgggtacagc gaactgctat gagctggtat gtttacagat tgactcattc
     3661 tccttttctt cttggtgtgg ttgccttttt aagtatgata cctgctctat ttataagtcc
     3721 atttgctgga gcttgggctg atcgctggga ccgtaggaaa accttaatcg gaacgcaaat
     3781 cctatttatg ttacaggctt ctcttctggc agcaggagtg ttaactggtt ttataaatga
     3841 gctgcgttgg tggcctctcc ttgttttggc attgtttcag ggttgtgttg atggtgtaga
     3901 tgctcctttt aggcaaaatt ttgtgctgga cttagtgggg aaaaagtcct tgctacctaa
     3961 tgctattgct acaaattctg ctatgtttaa ttctgccaga ctgctgggac cctctattgg
     4021 cgggataatg atagtattgg tgggtgaagg catttgtttt ttaataaatg ctctttccta
     4081 tctggcggtt ataggttctt taatggcaat ccgtattcag tatcgtaagc ctcccgaaag
     4141 caaagagaat atattcgcta aaattaaaga aggatggagt tattcctggc gtaatcttcc
     4201 cattcgctgg ctaatagcaa atcttggtgt gtttatgctc tttgctatgt cttatacttc
     4261 tgttattcca gtgtttgcca gggatgtcct taagggaaat gctggaactc agggtctttt
     4321 attaacctgt gcaggcgtgg gcgctttagc gagtgctatt tatatggcag gacgcaaaac
     4381 tatcaagggt ctaccctggg tttccattat gatgggttgt attggaagtc ttgcattaat
     4441 aggattttcg cagagtacca acctctggtt gagtatgtta atgatggtct ttgtagggat
     4501 gggattgacc attcaaatgg cgataactaa cactttatta cagacaatat cttatcccag
     4561 tatgcgagga agagttctaa gtgtttatac tatgacctta aattctctaa ctccttttgg
     4621 aagtttatta cttggttggt taactcggga aattggacct cgttatgcct taactatctg
     4681 tgcagcaatt tgtttactat gggttttgag tgggattaaa atggttccca aagtttccaa
     4741 aaacattcta agaatgctgg taaagaataa aagtactgaa ctctatagac ccattaccgt
     4801 tcatccagta tattcagaga cagtataatg aaaacttttg tgttctttgg agataaacaa
     4861 tgcgattata gaagtctaat ggctaatcaa aaagactttt atgtgggcga tagattgatt
     4921 ttagcgagtc aaaccgttat tgcagaacaa gtagcaagcg ataaagtata cctatgttca
     4981 aagaaagata atggtgccgg ttttggttct cattctcagg taccgggtgt tgatgctcta
     5041 attacagcaa ttcccaatca atatctttta atacgtactg cagattgttt cccaattttg
     5101 ctggaagata aaaaacagcg tgtagttgct gctgtgcata gtggaagaga gggaacccga
     5161 ttaaatcttg taggtaagac cataaacgct ctgaaagaaa aattggaggt tatggctcag
     5221 gacttaattg cttacattgg tgcgggaatt tgtggggaac attatactgt tgatgagggc
     5281 acctggagat tttataatga gtgtatggaa caacaaggtt tttttatcgg gaaagttcct
     5341 ttttgccata ttgaccttag agcagggata actcagcaac tccttgcagc tggagtccct
     5401 tcaaatcaaa tagttcatat aaatacctgc acatttgaat cacctctata tttttcttat
     5461 agaagggata aaactgataa tcgccaaatt agccttattg gacttataga tgagtaaaat
     5521 tctgacaaat ccggatggga atattaaatt agagcttaaa gatgataagc tctctgcctg
     5581 gttaaccatt aagaaagaca ataaacttat agatgaaaag gatattctgg acttattaga
     5641 acgagctgga attaagaatg gttttgaaga agcacaaaga tatatgcgga agcatagtat
     5701 ggaaaaggat tttgattttc cttttcccat agctatgtgt tcccgagtgc agggtgaaac
     5761 aaaacttaac tattatttca atctgaatca agcaaaacag agacaaagag agattcattt
     5821 agatgaccta cctaatctca cccgtattga agagggcact gtgattgctg attataacaa
     5881 tgatatcttt gatcgtcagg gctccatata tgacatttac ggagaaatgc tacctgatga
     5941 agaattggat ttacaggcag ctcaacaaat tgcaggtgag aatgttacct ttgatataaa
     6001 tcgtcaacaa tatttagcta aatgtgcagg gattcctcgc gttgatgagg aaggtagaat
     6061 ttctattata gataaagtta ttcttaagga aggcatatct tatcctgctg aaattattag
     6121 agcacctatc tctctggaag tggttggtga tatcataggc gcacaaatag ctgtagcagg
     6181 taaactaatt atccaggggg atgttgtgga tagcagaatc agttgtcagg acaatatcca
     6241 ggttcaagga gatattaaga actgtgagat cccaggttta gaaattaaag gcgatgttca
     6301 ttgcaagaag attatatgtt ctaaggttct atgttatggg gaattgactt ttacggaact
     6361 gattaaggat agcgaaatta ttgctaataa tggcatctct ggaccttcgg gatggttaca
     6421 aggaggacat acggagtcag gtgggaatat tgaattacaa aacttaggtg atcctgaagg
     6481 gaaactaact gaagtagaaa tttctatcag tccctggcat aaagctgccc taatgcaact
     6541 aactcgtaag atgatacaac ttaaaaaaaa tccactcact aatgctgctg aaatagaaat
     6601 attaaatgaa agaatagctg cttgtgaatc cgaattagat aatgctttaa atgagtttct
     6661 ccaacgtcct cctgaagata aattaaaaat ccgggttact caggatgttt tccctccagt
     6721 taatttacgc atattgaaaa atgaatatat aatcaaggat aaaaagccaa gtttagaaat
     6781 tatagaaata gattaaagga ttaatgatga aaaggataat gattatagtt tgtttagggg
     6841 ttatgattat atcctgcact gcactaattc cggattttac caccgatcca gccatttcac
     6901 ctgatggtaa tcagatatgt tttgtctatg atggagacct ctggttagtt cctttccagg
     6961 gaggaagtgc taaaagatta actgccacag gggcaaagga atggggtcca gcttggtctc
     7021 cagatgggaa actgatagct tttaactctg atcgggacgg caatgttttc ccttatatta
     7081 tttccgcaac aggtggttca gcagttccag tcatagaaga gagctacacg gtatgcgatt
     7141 ggtttaataa tggtcaggaa ttattatgta ctcgttctac tctggaattt ggcacttctt
     7201 tttatagact ttctctttct ggaaagagac ctgagttaat tgctgagatt ggagacccat
     7261 ttgcttctct ttctcctgat aattcctgtc ttgtttttaa ccgctatggt tatccttata
     7321 gagaagctta taaaggaagc caggcaggag aactctggaa aatagacctt tcctctaaga
     7381 cctatactcg tttaacttac acagattaca cagagcgcta tcctcgcttt tcctatgtaa
     7441 atgattattt gtattattgt gcatcagatg aagaaagatt ccaaatttat cgggttacct
     7501 ccctggattt ctctaaggtt gagcaaatta ctaatttaac agattggtct gcccgagata
     7561 tatctatagc ccgtcagaat gatcgccttg tctatgaata ttttaatgaa atctggaggt
     7621 gggatccaga aacagggatt gctgctcaag ttccaatcct cattgacgag gatatgtgga
     7681 ttccagacct acatcaagaa gtagtatata acaatattga agattttgct atctcttcgg
     7741 atgaattgct ggtgggcttc acctataaat atgatgcttt ttttatgcct cgtaaaggtg
     7801 gtaaggttgt ccagatagat acgggtcaga ctgctattgg agatatggaa tttttggacg
     7861 ataaccgcac tttggtcctt atcagaatgg ataaggggat agataaactt tataaagtta
     7921 gatgtgatag tctcttgcag gtaactccca tagattggtt tggtgcagat agtttggatg
     7981 ttgaaggttt atataaagat ccttctggta aatgggttat tttttatggc gacaaattga
     8041 tgagtgggag aatagcagta gccgattcaa acttatataa tatccgtcct atcaataatt
     8101 ctcgcgctgc tctcaatatc tttgcaatca atagcagtgg tgattatgga atctatgcca
     8161 ctaccagaga agatgtttgg attagagagt tgtggcttta tgactttaat actcaagaaa
     8221 gtaaaaagat tatgaacgat gatgcctgga ctcgttcaat gctctggact cctgataata
     8281 aatcaattct tatgagtaga agtaatggaa tctttcgtct ggatttagca cctcgggatg
     8341 agtttgaata cgaagttgat aactggaaag aaatccttaa tccccaagtt acgtctaaag
     8401 ctaccaaaac tgtaaatcaa gaagaaaata gagatcagga ttctactcct tcttcaactg
     8461 aaaaattaga aaaacaggag ctaaatattg tctgggaagg cattgagaaa agattgtatc
     8521 ccatattatc tgaatcaaac agaatcctta gtctcggaaa agttattgat gatagcacct
     8581 tttattatct atcttattcc cacgatgaaa ccgcttctta tcagcttaaa aaggcaaata
     8641 tatacggaaa gaattcaaaa accattcaaa atttaggtca aaacataagt aatcttcgct
     8701 gggtgggaaa ttctctttat ttcttagaaa atggcaaatt aaaagcttat aatcttgata
     8761 aacattctat aagtgaagta actgcaaact acgactatag tttcagtgaa agtaaactca
     8821 atctgagagt gtttgaacag gtttggggtg catttggatt gaatttttat gatcctaata
     8881 tgcatggtaa aaactgggag gacttgttta aactttatta tccctatgca gaaagagcac
     8941 gttctataag cgatatagag cagatagtaa atgaaatgat aggtgatgtg aatgcatctc
     9001 acacaggatt ttatcctcgt gctcaaaagg tcaaacctcg tcctcctcgc gcttatcttg
     9061 gcttagaatt tgattatagc caaattctgg aggaaggcat cccgattaaa gtagtttatc
     9121 cgcaatctcg cttagctgat tattatcata ttcaaaatgg tgatattctt ttagctatag
     9181 atggaactcc tataactcct tattcttctt tggataaact cctggcagga aaagccggta
     9241 aacttattaa attattaatt ctaaaagagg gtcagaaaat tgaagcggaa ctgaatggac
     9301 taacctggtc agaacagcga aagctatact atgattataa aacagaacga agtaaaaaga
     9361 tggtaaaaga attaactcaa gaccgcatag gttatgtgca tataccagcg atggggaata
     9421 ctgattatga aaatttccgc cgagaagtat tccgagacaa tgcggataaa gatgcgctta
     9481 ttattgatgt aagaggcaat tccggaggat atatacacga taaactcatc tctatgctta
     9541 atcaaaggta ttacgcttat tctacttccc gtcgttatag tggaaaactg aatcctgaac
     9601 ctcgtggtat ttgggctaag cctactattt tacttgtgga tgaacattct ttttccgacg
     9661 gggaaatatt tccagccgtg tatcaagaat taaaattggg gaaaattgtg ggacaaccta
     9721 ccagtggttc agtgattgga acctgggagt ttagattgct tgatggctct tctatgcggt
     9781 tacctggttc tggatggtat cgtctggatg gtacaaatat ggaagggaat ggagtccaac
     9841 ccgatatagt agtagaaaac aaacctgaag atataattgc aggaaaagat gctcaactat
     9901 taagagctat tgaagagata ttgaaagaac ttaattgact tcattggaga tagttctatt
     9961 ttaataatat aattcaggtg ctcaaaacta aaatttgttt aaaatataag aagagatata
    10021 aaaattaaaa ggatggattt aatcaatggc ggctgaaaaa gaattgcaaa atcttctcct
    10081 gagagcagaa gctttgatga agactaactg gatttatgct gtgcaattac ttaataaagc
    10141 agcggaagaa aatccagaag acccacgtcc acttattgct ttgggagatt tttatcagca
    10201 acgccagctt tttaacaaag ccgtcaaata ctatcaatcc gctctgaaat taagtccaga
    10261 tgatgataga ttgaaattaa tcattggtaa tactcttttt tccgagggag agtatcaatt
    10321 agcgattgta tattatgatg agatagaaga ccaaaatgta gatgtccgtt ataataaagc
    10381 cttggcactg gcatacttgg gaagaaaccg tgaaagcatc aatattatgc gtgacttact
    10441 ggatttaata gataataatc cttttattta tttcctttta attgagcagt taatgcatat
    10501 agaggagtat gaagaggcac aaacatacat tcggaaggcg gaaaagagaa tcggagagca
    10561 ccgtcatctt attttattaa aagctcttat ttatgctcat tttcagaatt ggttacttgc
    10621 ctataatgct ttttacaact atgaacaaag tggagatata gttcagagtg aacacattta
    10681 tatctatgcc gattgtgcgg taaaaagtgg aatgtccaat agggctatcc agattctgga
    10741 aaaagggctt aatgataatc cctactcaat tcctttatat gaagaactta tccgtctact
    10801 tattcagcaa aaacgtatta ttaaagctcg ttattatatg caggaagcca aacatcactt
    10861 tagtgtgctc agtcccttac ttcaattgat ggatgctcgt ttaaatagaa tgtaaagtca
    10921 acctatgcca gtttttttac ttagcaatcc gttaaaacat tatgaatggg gctcacctta
    10981 ttatattcct caactgctta aaatggaacc ttatccagac aagccagtag cagaaatgtg
    11041 gattggtgct catccagctg attcatctat cctgagtgaa gttgaaggtc aaccatcctt
    11101 acgagaatat atcttaaatc atccagttga agccttgggc aaagccaaga atctttataa
    11161 tggcgaactc ccttttctgc ttaaagtctt agcggcagag aaggctcttt ccatacaggt
    11221 tcatccttct gcagaaaagg caaaggaagg tttccttcag gaaaataagc ggggtatacc
    11281 actggactca cctaccagaa actatagtga ccctcatcct aaacctgaat tgctgtgtgc
    11341 cctcaccgag ttcaaggctc tctgtggttt ccgaaatcca gagcaaattg tcgctaattt
    11401 ccgggcggtt aatttagata agttattccc ttctctctca actttagcgc aaaaacaaga
    11461 tgaagccacc cttaaaaact ttactcaaga agttcttagc gtatcaggcg aaaaattaaa
    11521 tcaaactctc agaatattag agcattcact taatttaaat agaaatctac cggaagatgt
    11581 agtctcagct tttaacttaa ttcggaaaca ctttaatgag gatgcaggat tgttagcacc
    11641 tctttatttg aatacctaca ctctcaatcc aggagaagct ctatttttag aacctggggt
    11701 tatgcatagt tatctgggtg gagcaggaat tgaaattatg gctaattcag ataatgtctt
    11761 acgggcagga ttaactccca aatataaaga tatcccagaa gtattgaata ctctggtata
    11821 taaaccttat gttcagaagg taatcttacc taaaaatata gacgatattt attttgtata
    11881 ttctgttccc gtaaaggaat ttgaactcca aagcctgcat attatgccag gactaaactt
    11941 gaaattacag cttcataata tgcctgtgat aatactatgc tatcagggtg aagtcactat
    12001 cctggaacat caaaaaattc tttctcccgg aaaagcaact ttcatcactg ctgacatccc
    12061 tgaactttct cttaagggtc agggaattct ctggttagca actgttccta cttgattaaa
    12121 actgaaactc tatccccaaa gacattgatt caattatttc atcatttccc ttaatcttgc
    12181 catccctatt caggtcgcga taatattcgt tataacgaag agcaagatta aaagagctac
    12241 tcaagttata gacaatcttt ccgccaattt cagcattttc attacgtaag gtatcaaaac
    12301 tgatataatc tacattggat tgcccataaa caatggaagc ttcttgtaat tttggtatta
    12361 tattaggaat aacagttaat tcaccccata aggatttacc cttggtcttg ctatcattat
    12421 acatatcctg ataggtaact ctcaaatagg caaagtttcc cagattggct ttaagactgc
    12481 caaaccagcc taaactggaa gtgacgcctg ctaattcaga ttcttttgtt tttaaagaat
    12541 aacaacgacg accattttca ctttctattg tagtatatac cacctgagac ctttgctcat
    12601 cataaagatg attgaaaaat ccgggaagaa actggtcttt gaaactacgg aattctaaat
    12661 ttaaattaaa gattaagaat ctggctgcaa atccagggaa aattataccg ctaccataat
    12721 cttctatttt tgcatattca gcataatgac ttaatccaaa actcttctta tttaaaatag
    12781 gtagttcata atctaatcca tagattgtta tctcattgct ttgatctatg ggcatagctt
    12841 catcaggata tacttgagta tcaaacggat aattaggata attatcggca ataccaggga
    12901 attcactgtt cacataagga ttaagtgaag gatgatctaa tatagaattg ccgtttaagt
    12961 ctatatccat attatctggt atgccatcat tatcagtgtc taaccagtaa taaggatcat
    13021 tggggaattt atcgtaaaca tcgggatagc catcaccatc agaatcttca tatttgccat
    13081 aagggtctct atccatacca atactggcac ctatcttaag gtttttaact atagggatac
    13141 cagttggttt taggggttgt atataaagat gcccaccaag aatctcattt ttggaaacat
    13201 catgagtaaa tacctcaaaa cctgctccca gtgacttgag attggttcca acataagctc
    13261 caataggttt aacattagga taatgtaaca tattggagta ttcatcaaat atcaatccgt
    13321 gacccatagt ataatcattt atacttccta ctttaaagta aaaaggatcc tgtctttggg
    13381 caaatctaaa atagtaaagt ttacttaagg catcatccca ggaatcccaa tcctctttac
    13441 gaacattacc attactatct attagtaaat caatatccag tcccaaacca aatttccaaa
    13501 tagtgatttc tggctgaaaa cgaaattgag tgtaataagt gccatcaatg aggatattac
    13561 ctaaggtgcc acctaaacct aaagcaggag aagaaggagt gaattctcta cctgtttctt
    13621 catccatcat accttcatcg ttaaaactct gtgcaactaa taaaaacggg ataagcaata
    13681 gaaaaattaa actaatctta cgcattttac acttccttgc tttaatttaa tcgttataca
    13741 aaatatatat tcttaattta tcagttctgt caagcaaaaa acttatgtat gtagattctc
    13801 agcagaaaaa ataccttctt ttgactgcct ttctcaaatt aagatggggt aggtcataaa
    13861 tttctaacat atccatttct ctttcggata attttaaaag tttaacgaat cgtattctta
    13921 tatgcaagtt agaaatatca tccaaaggag aataagtgga ttgggagcat aaagttatag
    13981 aaataagaaa aacattattg gaattagggt tgatgtagta gaattaatat aattctgagg
    14041 acttatattt aggaataagg agaagaattt tagattttat agcaactatt gaatatcaca
    14101 ttggaagaaa ttagaaaata gaaaagatga ggtttattta gacttatctt attataatat
    14161 aatcagatac tatctctaag ttcacactta cctctctctt aagaacttaa gcaagcaaga
    14221 gctaagggag agccaagata gagatgaaga tttacctatg aataatagtt ataattttag
    14281 aaaatgatat ttattaaaaa agtttttata tgaagttatt tttctataag ttctggttgt
    14341 agacaggcaa acctagggtt aaaataattg aaagattaag ttgttggatt aaatcagata
    14401 aggttatggt cgttccttac ttttttaggt aaagtttcaa ttttaatcgt tattttaata
    14461 ttaagatgct ttcatataaa taagcttctt tttattaggc tatattataa agtcagttaa
    14521 cctcaccttc ctgattttaa tttaaaatac tgacttctac ttctatcttt ttctgatagg
    14581 agttaataga tataatgata attattgcaa ataattagct cagtagtcca tctcttctta
    14641 atagagcttc agggtcaggt tttctaccgc gaaaagcgac aaataggtcc ataggatgga
    14701 aggcatttcc gcgagctaag atatattgac ggaaagcatt agctacttca ggattaaaga
    14761 tacctttttc tttaaaaaga ctccagacat ctgcttccag agattcagcc catttatagg
    14821 aatagtaacc agcggaataa cctcccgcaa agatatgagc aaaagcacag gagatattgc
    14881 ttttgggaac tggaggttgc acttgaaaac gagcagtcac ttccttttca aaggcatcta
    14941 catcagaaat cttttctact ggtgtgatat gccaggcaag atccagatat ccataacgaa
    15001 gctgattgat attagcgagc ccagcttgga aattcttggc ggctataacc ttttgcagta
    15061 attcttctgg aagaggttca ccggtttcat aatggcgagt aaatagtttt aatgcttctt
    15121 cttccagcaa ccagttttcc atcaattgac tgggtaattc tacaaaatcc cagagcacat
    15181 tggttccgct taggctttta tagtatccat ctgccagaag agaatggaga gcatgtccaa
    15241 attcatgaaa aatagtgcga gcttcatcca gaagcaaaag tgaaggttgt tctggagttg
    15301 aaggtgtaag ggatgctaca atagccactt gaggtctacg aataccgtcc gaataaagtc
    15361 ctgcggtctg gaaagttgtt tgccaagcac ctcctctttt tgtctctctc gggaaaaggt
    15421 ctaaatagag taagcccaga taatttccat ctctatcata agcttcccag gtgctaacct
    15481 caggatgcca ggttggtata tcgttaacct gtttcatttc aatgccataa agtctattag
    15541 ccacaataaa taagccactc aagacatctt ctactttaag ccagggacgc agttcttctg
    15601 ggtcataagc atatttcttt tcttttaatt tattggaata ataggctaaa tcccagggtt
    15661 gaaaatcagt gatattatca atttcacggg caaattccac tacttcttta tattcatttt
    15721 gagctgcagg ataagccact tgatatatct tctccaggaa atttatcaca ttttgaactg
    15781 actctgccat acggtcttca agcacataat cagcataagt gggataacct aaaagtttag
    15841 ctcgtctttc tctcagttta agcagttgtt tgatgatttc ttgattatca aatttatcgt
    15901 taaaagcacg agaattatat gctatctgaa tctgacggcg caattctcta tttttgcaat
    15961 aagtgagaaa aggaaccata ctggaaggtt gaagagtaaa tagccatcct tcctttttgc
    16021 ctttgacctt tgcttgatgg gcagctcttt ccaaaacatt ctgagggata ccttccacat
    16081 cactgggatt agtgatatat aattcaaaag cattggtggc agcaagaaca ttatcactaa
    16141 atttaggtcc cagtttagaa gcttccatct ctatttcagt taattcttct ttttcctctg
    16201 gagttaataa agcgcctcct ctgataaatc ctttataaac acggtcaatg agacgataac
    16261 gctctgcctg ttgcaaatat tgcttttcgg agaaatcttt gggaatttgg ggtttaggcc
    16321 tattctgaac ctctttttcc cacacttttt taactcttga gaagataacg gggtctgtca
    16381 taatagaact actaaattct gctaaaagag gagaaatctg ttgtgccaga gctttaaatt
    16441 ctgcatccga ctcagcagaa agcagattga aatagatgct ggctacatac tccagctttt
    16501 ctccactcaa ttccagagct aaaatagtat tttcaaaatc tggttctgca ggattatttt
    16561 ttaaactttc aatctctgcc tttgccattt tcaaagcttc ctgaatagca ggcataaaat
    16621 gttctgtttt aatctcatca aatggtatag cctgtaaacg atgttcgttc ctttctttca
    16681 ataatggatt ttccgttttt ggcataatag ttccttttat atttgtttta tatttatcag
    16741 aataacaaga gttacaaaat caaacaaata ttttttaggt taatgcttgt caatttgaaa
    16801 atgattgacc ctttagtaaa tatattaatt gacgacctaa tcctttattt tatctataaa
    16861 atgaggatag gtaaaaaata taaaaccatt taactttatg acaatacaaa cttgttattt
    16921 atttaataac aatcagataa agcgaaatat gacattttcc atctctaagt aaaatgaagt
    16981 aaaaaagatt gacggatttc ttattttata ttttaagtat aagtagatga tttataaatt
    17041 aataaagagg ataagataaa tacaataagt aaagcgtgaa aaagctcttc cagtatactg
    17101 gaaaataaat taaaaaggag agaacaatgt taaaaaaatt agttttttta atcgtcgcag
    17161 tcctatcatt tacatattta ataggattaa cagcaggtga tatcgctatt ttaggagttc
    17221 atactgatac tccaaaatca atgatttttg ttgctttagc cgatattcct gctaatactc
    17281 aaatttcttt taccgataat ggatggaatg ctaccaccca acaatggaga ggtaatgaag
    17341 gaactattgt ttggaaacat actgagttag ttagtaaagg tacctgtata actctttcca
    17401 ttaatacaag tccttatagt gccactttag gcactgttac aactcatgca aattttagtc
    17461 ttgctacagc tggtgatcaa gttttagcct atgaaggcac cactcctccg acatcaaatg
    17521 atgcttctat ctggttgtat ggtatctcaa cttctaattg ggtatggggc aatagttcta
    17581 ctacatcaga tttaccaaca gccttagcta atgcagcagt tgcttttacc ggtactacaa
    17641 cagatattga taacggttat tttgctaacg gtagtgaacc tcaaacttct gtatcagtat
    17701 caggaacaaa ggcacaatta ctttctttat ttggcgatag aaccaaatat tacaccaatg
    17761 ataatggtcc tctaacattt cctacttata ccataacagt aggaagtgaa ccagttattt
    17821 ccgtatctgg aacacttaat ccctttagta caatagtagg aactccttct ccagcccaaa
    17881 actatactct aataggacaa aatttaacag ctaacattat tgtaacttcc cccacaggtt
    17941 ttgaaatatc tactgatggt agttcttatt catctaatct ttctttatcg cctacttata
    18001 atggttcaat ttatgtaaga ctgaccgggg caacaatagg aacatttagc ggcaatatca
    18061 ctcataccag tacaggtgca gctcaagttg acctacccgt aactggaact gttttagaga
    18121 cccaacctcc agtactgcac ataactggaa ctttaactaa atttactatg gaacagggaa
    18181 ctccctcaga aagccagggt tataatttat atggagaata tcttactcag aatatttcta
    18241 ttataccacc agcaggatat caactttcaa cagataatac aaactggtca ggtcaattat
    18301 cccttaatcc ctcttttaat ggcacagtct atgttcgttt aaatggaact aatattacaa
    18361 gttataatgg gaatattgtt cataccagtg gta
//
LOCUS       DAORZF010000035        18245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_2527_length_18245_cov_385.046124, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000035 DAORZF010000000
VERSION     DAORZF010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 18245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18245
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2527_length_18245_cov_385.046124"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            259..735
                     /locus_tag="PK015_05190"
     CDS             259..735
                     /locus_tag="PK015_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HOL33895.1"
                     /translation="MVYYRSQVENIAKKLCAEQHLALYDIEEKMSAKGRIITVYLTKI
                     GGVTVDDCAYFSRSLSDELDELDIIPERYFLEVSSPGLERVLKLKSHYASAINEKVAV
                     QWTEGDEKHSAMGTLIEVNADTITLDDRGKIVEINFKDINRAKTVFLDKERSEKKR"
     gene            732..1967
                     /gene="nusA"
                     /locus_tag="PK015_05195"
     CDS             732..1967
                     /gene="nusA"
                     /locus_tag="PK015_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOL33896.1"
                     /translation="MSTNILDAVVKLAAIKQLDKETIQAIILESIYNILNKKLEPENQ
                     LELIVDEDTNAIKANFLCKVVEVEKNLGEISLDEARKIYGKDVEIGQYVPKTMAMYEF
                     EPKLIKTAQKAIQDKIHQLEEEKVRNDFDKQKNTIVSGKIKSIDDNGNYRIDIGYSDA
                     ILPVDEQIENEYYRVGDNIKAYVVNIQVHKNNNAIILSRTNPEFVKKLFETEIPEIYD
                     GTIKILKIVREPGVRTKVEIESTDPKLDPVAACIGIKGTRIDSIRRELHGEQIDLVVH
                     DEDPEKMIVRALGLENVKRVILERNRAASVIVDDEDKVMAIGKGGKNVKLAAKLTGMK
                     IDIYTMDEFEEKMAKERRTISHITELDGVTPKIAEILRHAGYTSVQDIYAASIEELCN
                     LEGVGEKTAQRLKEAAKDF"
     gene            1967..2266
                     /locus_tag="PK015_05200"
     CDS             1967..2266
                     /locus_tag="PK015_05200"
                     /inference="COORDINATES: protein motif:HMM:NF016204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF448 domain-containing protein"
                     /protein_id="HOL33897.1"
                     /translation="MPNKHSKAQHMPQRTCAICKSKKDLDELYAFLIMPEGIIIDPKR
                     RLQRRRHYICKNINCLSNLEEWEKRYFKKHYGIKKDWSELDLQSKNSVQGVNNGQ"
     gene            2256..2579
                     /locus_tag="PK015_05205"
     CDS             2256..2579
                     /locus_tag="PK015_05205"
                     /inference="COORDINATES: protein motif:HMM:NF013417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L7Ae/L30e/S12e/Gadd45 family ribosomal protein"
                     /protein_id="HOL33898.1"
                     /translation="MDNEEKKSHIINLMQFARKAGKMVSGTDACLRDLRKNNLKLIII
                     AEDTSPKTVKRITDARNVLENFVPIIRLGTQKELSMALGLPVSGIFGIKDGQFAAKLL
                     EYYSN"
     gene            2596..5280
                     /gene="infB"
                     /locus_tag="PK015_05210"
     CDS             2596..5280
                     /gene="infB"
                     /locus_tag="PK015_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOL33899.1"
                     /translation="MQIRVHELAKELKISNMALRKHLNDMGVNVKSHMSLIDEEIADK
                     IRKKYYEQVDAEKRAEKDRKRLQEMRQAAKANKTEKAPQEKPRETPETKATKPSTKEY
                     AKAAKTQPALEEEVKKTEAFESVAPPDTTEPAPVPEPKQAEPVEQAPSKPKPEEMLPK
                     GKPPTRIIVPHQQATPVISKGKKTTPKPKPKPESVSRERPTTFKKSPREERKKEEPRK
                     KPPVTTTPLPIEPVETEKEPEIKKFGKVRVNHEELGEKSKHKRAIVSSVKKSKQKEEF
                     PIDVDEAEISRNIKKILQKTRKKKYHKEAQATSENTENKVIIREFTSVSELAKILNVP
                     PADIISKFFAMGQLVTMNQRLDRDSLEMICDEFNVDFQFEDEYGVDILDKEKELYADA
                     EYEPRPPVVTIMGHVDHGKTSILDYIRNTNVVAGEAGGITQHIGAYQIEINKNKITFI
                     DTPGHEAFTAMRARGANVTDIAVIVVAANEGMKPQTKEAIDHAKAAGVPIIVAINKVD
                     LPDANVNRVIAQLLDYGVMLEQYGGDVPWCTTSVVTGEGILNLIELILIMAEMMELKA
                     RKKVPGSGVVLEAEKDPRMGSIATILVQEGTIKKGDIIVCGPTHGRIRRIENERGQEI
                     KQLYPSDVGRIFGLNDVPKAGDLVNQVDSEKTARAISTERNQIRLEREKYQNQASLQN
                     IFERIRDEQLKELNLIIKADTDGSVEALADSFAKISHPQVILNIIHKSVGGINEADVS
                     LAAASNAVIIGFRVRANQQAKKMAEEEKVDIRLYQVIYDAIEDVEASLEGMLQPEYKE
                     HIIGTASVRQVFKIKKVGTVAGCQIESGYVRKGCKVRVYRNDVMIAEDNLATLKHYAE
                     DVEEVRAGSECGITLENFSDIKEGDILEFYVLEESAKK"
     gene            5284..5787
                     /gene="rbfA"
                     /locus_tag="PK015_05215"
     CDS             5284..5787
                     /gene="rbfA"
                     /locus_tag="PK015_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOL33900.1"
                     /translation="MKSYKIPRLQEELRKILNITLTTKINDPSLAWVTITDVVLSRDL
                     KYAKLYFSHYNNPASHENIRERLIKTSGYFKKQIAGAHIMRTIPELSFYYDDTEDRAA
                     RVETLLASLKDKYNDDEEDYENFDEDFDWDEEYEDEDDDLFDLDEDDDDIFDDIIDID
                     EDEGENK"
     gene            5873..6886
                     /locus_tag="PK015_05220"
     CDS             5873..6886
                     /locus_tag="PK015_05220"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOL33901.1"
                     /translation="MKNLNNIQELKDILNGFLNASKSVAIMTHTEPDGDGFCASLALQ
                     RLLSYQGIDSEIIIDADSQLERFKFLMEGAKLSIFESGNYYETLIVLDCNSYTLLGNR
                     DELLRTARKRLVIDHHIPTDKELDSDYTFVDITEVCTGSIIYKMFKSEINNLPPAEQR
                     FIANNLYITLINDTNNFTNSNTDAKSLSLAAELVDLGIVPSDLYKAFFLNNTTSQMKY
                     LGEVLSGMELYNEGQILFLYSSLDMQKRNHLSTNAISVPRFVQGIKGVEVIAFLRQDD
                     KEAYNLSLRSDTVDVNAIAVSYGGGGHRSASGAMLYGELEELKSDLISKLKQALTEFP
                     NNV"
     gene            6879..7745
                     /gene="truB"
                     /locus_tag="PK015_05225"
     CDS             6879..7745
                     /gene="truB"
                     /locus_tag="PK015_05225"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOL33902.1"
                     /translation="MSENQGFLLIDKASGSSSFALVKQLRALTGISKIGHTGTLDPFA
                     SGLLIFALGSYTRLCQFLELQDKEYEAVLKLGEQTETGDLEGKVIAVSENIPNQLDKE
                     ALREQILQIKSLATPAYSAVKIKGKPAYSYARKGIKVELEDRPVHISKFEVTDYSPPY
                     LSFCCKVSKGTYIRSLGELIAQRLETVGHCVNLRRTAIGHLSVNQASKIEDITKENFT
                     SHFFPIEELFPDFEKLYLPEKELIHLSNGNPITNSGKDYEKILIFDSKGSLQGVAKRK
                     DGIIIPYINLKS"
     gene            7747..8655
                     /locus_tag="PK015_05230"
     CDS             7747..8655
                     /locus_tag="PK015_05230"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOL33903.1"
                     /translation="MKTVLSMGNFDGLHIGHKKLLSRVRELAEERNLKSVVITYDNHP
                     AQTLNNNSYPYILLPPEHKRTKILDLGIDEVQLLHFDEKLAKTSAEDFLREYLMAKYN
                     PAIIVLGYDSHFGYQRRGNYQFLLEQSKNYGFEVEYVEPVLYNNRIVSSSLIREMLIS
                     GNLYVANRLLGYPYTLYGTVGRGRGIGKELGFPTVNLQLEDTCQLVPYNGVYFSIAQT
                     GDLVFYGLTNIGISPTLKHKDKAEIETYLLDIKRDFNDYTFAVSLLHYLREERMFPDK
                     DALIRAIEKDVALAKKLVDNKIICDF"
     gene            8643..10553
                     /locus_tag="PK015_05235"
     CDS             8643..10553
                     /locus_tag="PK015_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOL33904.1"
                     /translation="MRFLITFAVLLFVSFSFALDFVPGSGSQVIPEDSKEYNFAYHQG
                     TDDYHFYGSDKWAVFFDFASVYPAAEISQFLVSKALIYLPQIGDSIKVELFTNGYNSP
                     GTLLTWNKVLVSSNWLEITFPQTVQESSLWMVATYSTSFIGTFVSASYGSGTHSYYSN
                     TNVPQSPYYQSFANAGFSAELLFGLTGEFVLDSQIIDLELTYFNLEGIVEPGQTVAPT
                     FAIYNHSSIPISDAAIHLNVYSPDPDFAFFDELPVTETIAPHSLYEFNDQNPDFLEHQ
                     FTLPERPLQLKLRAALVSSHNDNDPQANNIKLIHRFSFADSYPIFLIENFLRWQNASQ
                     ISWSQDQYNLPQFHILNYFPILGDSLSNLAAQNRFNWYHFNSLPRTVINGNLKINGFS
                     SNYGNQFTQLCTQAQNSKTFISSADCRIISEENSDQLSASLTLNNAHTQLYSSAVEYD
                     IVNLSRLFVGLFKKETFNNSERWVIDRWIVQNQALNGSLNAAQSLTLDFSIPLNNIPL
                     NELEESYRLYYWLQMSDTGKILYSSFAEFSNFVPVNDINYSIPRLNITSNPLRRGEQM
                     KISLNNGKQLGRVQVYNLKGQKIKDFYTTQPEIVFTSDEFPTNGIYFLRFPELPAAKA
                     GNPTTHKIIILK"
     gene            10560..11510
                     /locus_tag="PK015_05240"
     CDS             10560..11510
                     /locus_tag="PK015_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOL33905.1"
                     /translation="MFEHINISDLKEHLGEEVVSFYLVTSKEIRQGKNDRYLYLRLQD
                     HSGSINAYVWKDFQKAAEEFSEGDVIKIKAMVNNYKDQVQLNVSQLRYADKSEYNIEH
                     FISSSSKPPEFLAEQFFSFVDKVENLWINKLLHRIFDDKQFFNLFLNAPAAKSWHHNY
                     RHGLIEHTVNVATLCDSVCTLYPVHRDLIIAGALLHDVGKVFEYSPAPNIDFTDLGRL
                     VGHLSLGDQFVCEKAKEIIGFPEEILLNLRHMILAHHGEYEKASVRLPQTLEALVLHL
                     CDNLDAQSVGVAQLLAAAPDDAVWTEYDKLNNRYYHLTKI"
     gene            11514..12299
                     /locus_tag="PK015_05245"
     CDS             11514..12299
                     /locus_tag="PK015_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOL33906.1"
                     /translation="MFQLAVLVSSSQGNASFIRTDETAILLDAGTPAKTIFNALQDLG
                     IDRYEIKGVVLSHEHSDHIKGAGAVLRYLKVPLYINEETYHQCQYRLGNIPAGINFFQ
                     TGIPFRINDLVIHPFSSSHDAVESCNFTFHRLQNQERKLGIATDLGFPTTLVVNKLKD
                     CTTIVLESNHDEKMLMEGPYPWSLKQRIKSPQGHLSNQQAVGLLSQIMHYGLENLILA
                     HLSETNNNPQLVWDTMNDFLQSIRSNVNLLIAEPYRHTPLINI"
     gene            12897..14795
                     /gene="gyrB"
                     /locus_tag="PK015_05250"
     CDS             12897..14795
                     /gene="gyrB"
                     /locus_tag="PK015_05250"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOL33907.1"
                     /translation="MTDNNYTSKNIKVMKGLEGVRARPAMYIGSTGIQGLHHLVYEVV
                     DNSIDEALAGFCDYIKVTITTEGEVEVEDNGRGIPVEMHQEMKLPALQVVMTMLHAGG
                     KFDQESYKVSGGLHGVGVSVVVALSEYLEVKVHKDGKIYYQRYERGKAVTDVMEIGET
                     DRTGTIIKFKPDSTIFETTEFSFDYLTARLRELAFLNKGVRIILRDDRTGREHDFKYD
                     GGIVSFVEYLNQNKKPLSPKPLYIEAKRDDLEFELALQYNEGYQENIFSFANNINTLE
                     GGTHLSGFKSGLTRAVNTYIKNADLLKNEKVSPSGDDIREGLTAVISVKLKNPQFEGQ
                     TKTKLQNSDLEGIVNSVVYEKLLTYFEEHPAEAKAITMKSIMAARAREAARKARELTR
                     RKSVLESSSLPGKLADCTITDPSKTELFLVEGDSAGGSAKQGRDRSFQAILPLWGKML
                     NTEKARVDKVLNNDKIQPIILAIGAGVGQDFDVSKIRYGKIIIMADADVDGAHISTLL
                     LTFFYRYMRPLIEHGHVYIAKPPLFMVRKNKQVKYVFTEEERDEAIKEFGEKGVFVQR
                     YKGLGEMNPEQLWETTLNPENRLLVAVKMDDAIEADRIFTILMGDEVEPRRDFIQTNA
                     RYVQNLDI"
     gene            14809..17403
                     /gene="gyrA"
                     /locus_tag="PK015_05255"
     CDS             14809..17403
                     /gene="gyrA"
                     /locus_tag="PK015_05255"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOL33908.1"
                     /translation="MEDKSRIIEVQIEDQMRQAYLDYSMSVIVSRALPDIRDGLKPSQ
                     RRIIYAMSELNLVPGGHFRKCAKIAGDTSGNYHPHGEQVVYPTLVRLAQPWNMRYPLI
                     EGQGNFGSIDGDPPAAMRYTEARLQKVTMELLAELDKDTVNFRSNYDETRKEPEVFPA
                     RIPNLLINGSSGIAVGMATNMPPHNLTEICNAVIALIDNPDLEIWELHKYIKGPDFPM
                     GGYILGTKGIMDYFLTGRGQLLLRGEAEIETNKNEQEQIIIRSIPYQITKTALIDKIV
                     ELVKDKRIEGISDIRDESGRDGMRLVIMVKRNADAQTVLNQLYKYTQLQTTFGVINLC
                     LVNGVPQVVNMKDMLNNFIEFRHEVILRRTNFELRNAEERLHILEGLRIALDHLDEVI
                     ATIRASQTPPEASEQLQEKFGLSEIQAKAILDMRLQRLTGLEREKIETEYEELLKVID
                     HLKDLLEHKEKRMQLIKEETAEIRDKYGDPRRTQILESYNGILNPEDMIADELVVVTI
                     THEGYVKRMPIDTYRVQGRGGRGLTAANLREEDFIQYIFVASTHSYILLFTDLGKCYW
                     LKVYEIPEAGRTARGKAIVNLVNLQDKEKVKAFVTLKDFNPEENIVMCTKNGMVKKTP
                     LAAFSHPRSSGILAIKLLPGDELIDARITEGNDDLILATHNGYCNRFSEREIRPTARF
                     TQGVRGIRLREEDYVVSMAVISQDLLMENGNALTKTILAISENGYGKRTRISAYSITR
                     RGSKGVITLKTNERNGHLAALMLVDENDDLVIITQEGMIIRQKVSDISIFERNTQGVH
                     LINLRENDKVVDITIIPKEPNEEELDREVEKAKNAPSMQASNTMEDVTEEQDEEEEFR
                     DEELPEEE"
     gene            17378..18019
                     /locus_tag="PK015_05260"
     CDS             17378..18019
                     /locus_tag="PK015_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33909.1"
                     /translation="MKSFLKKNDLICPLQKCKGASYFRVISCWFIFVISMLLLSGCAV
                     KPYVPVPGDFYTQEADWAIINTDSLNIFVHPQSYYGVAQNTASSYFTLYVRVKNKSKK
                     NISLNRSSFSILAGNQQFDYIPLEIILGSLQNYSLLRYYQDPFASPIPANYDKYLQDI
                     QQQYLELVNSYFSFGELLPGGSKEGYLFYDKKIEQYNSFELDVWGTKIKFVRK"
BASE COUNT         6134 a         3171 c         3684 g         5256 t
ORIGIN      
        1 ccttagcgga cataaaccat aatttatcta tttccaattt attactaact ttagccactt
       61 agttttaggt aagaggtctt ataattaggg gaagaaattt ctacttgaca gattaaatcc
      121 agattagatt ttgactttgc atacagaaat gaatataaat catttcggtg aagagagttt
      181 ttgtttaaaa agatagctaa gagaattagc tatttttttt agcataatat gtatataagg
      241 tgaggagaaa gatagaagat ggtgtattat cggtcacaag tagaaaatat agcgaaaaag
      301 ctatgtgctg aacagcatct tgccctttat gatatagagg aaaagatgtc tgcaaaggga
      361 agaattataa ccgtttacct gaccaaaatt ggtggtgtga ccgtggatga ttgtgcctac
      421 ttcagtcgtt ctctaagtga tgaattggat gaactggaca tcataccaga gcgctatttc
      481 ctggaagtgt cctcaccagg gttggaaagg gttcttaaat tgaagagcca ctatgcaagt
      541 gctattaatg aaaaagtggc tgtacaatgg acagaaggcg atgaaaagca ctctgctatg
      601 ggaactttga ttgaagttaa tgcagacact atcacactgg atgaccgtgg aaagatagtg
      661 gaaataaact tcaaggatat taatcgtgca aaaaccgtct ttcttgataa agaaaggagt
      721 gaaaagaaaa gatgagtaca aatatactgg acgcagtggt caaacttgca gctatcaagc
      781 agttagataa ggagactatc caggcaataa tactggaatc tatatataat atattgaata
      841 aaaaactgga acctgaaaat caactggaac ttatagtaga tgaagatact aatgctatca
      901 aggcaaattt tctctgtaaa gtggtggaag tagagaaaaa tctgggtgaa atttctctgg
      961 atgaagcccg gaaaatttat ggaaaagatg tggaaatagg acaatatgtg cccaaaacta
     1021 tggcaatgta tgaatttgaa cccaagttga ttaaaactgc gcaaaaagct attcaggata
     1081 agatacacca gctggaagaa gaaaaagtcc gcaatgattt tgataagcaa aaaaatacca
     1141 ttgtcagtgg taagattaaa tctattgatg ataatggaaa ctatagaatt gatattggtt
     1201 attctgatgc tatcttgccc gtagatgaac aaatagaaaa tgagtattac cgcgtgggtg
     1261 ataacataaa agcctatgtg gtaaatatac aggtgcataa aaataataat gccatcattc
     1321 tttctcgcac taatcctgaa tttgtgaaaa agctgtttga aactgaaata cccgagattt
     1381 atgatggaac tatcaaaatt ttgaaaatcg ttcgtgaacc tggagttaga actaaagtag
     1441 agatagaatc aaccgacccc aaacttgatc ctgtagctgc ttgtatcgga atcaaaggaa
     1501 ctcgtataga tagtattcgg agagaacttc acggtgaaca gattgatttg gtggttcatg
     1561 atgaagaccc tgaaaaaatg atagttagag ctctcggttt agaaaatgtt aaaagggtta
     1621 tattagaacg caatagagct gccagtgtta ttgtagatga tgaggataag gttatggcta
     1681 tcggaaaagg tggtaagaat gttaagcttg ctgctaaatt gaccggaatg aagattgata
     1741 tttacactat ggatgaattt gaagaaaaaa tggcaaaaga acgcaggact atcagtcata
     1801 taaccgaact ggatggagta actcccaaga tagcggaaat actaagacat gcaggatata
     1861 cctcagtgca agacatctat gctgcatcta ttgaagagct ctgtaatctt gaaggtgtgg
     1921 gtgagaaaac tgcccaaagg ctgaaagagg cagccaaaga tttctaatgc ccaataaaca
     1981 tagtaaagct cagcatatgc cgcagagaac ctgtgctatt tgcaagtcca aaaaagacct
     2041 ggatgagctc tatgccttcc ttattatgcc tgagggaatc attatagacc ccaagagaag
     2101 attacaacga cgcagacatt atatatgtaa gaatataaat tgtctttcca atttagagga
     2161 atgggaaaag cgttatttta aaaaacatta tggaatcaaa aaagattggt cagaactgga
     2221 cctgcagagc aagaattcag ttcaaggagt aaacaatgga caatgaagaa aaaaaatctc
     2281 acataattaa tttaatgcag tttgctcgta aagcaggtaa gatggtttct ggcacggatg
     2341 cttgtcttcg ggatttacgg aaaaataatc ttaaactgat tattattgcc gaagacacat
     2401 cacctaagac agttaaaagg ataactgatg caaggaatgt tctggaaaac ttcgttccca
     2461 ttattcgctt gggaacacaa aaagaactaa gtatggcttt aggactacct gtttccggaa
     2521 tattcggaat taaagacggt caatttgccg ccaaattgtt ggaatactat tccaattaaa
     2581 attagaggga aaaaagtgca aataagagta cacgaattag ctaaggaact taaaataagc
     2641 aatatggctt taaggaaaca ccttaatgat atgggggtta atgttaaaag ccatatgagc
     2701 cttattgatg aagaaatagc cgataaaatt cggaaaaaat attatgagca agtggacgct
     2761 gaaaaaagag ctgaaaaaga tagaaaacgt ttgcaggaaa tgagacaagc agcaaaggct
     2821 aataaaactg aaaaagcacc tcaggaaaaa ccaagggaaa ctccagagac taaagctact
     2881 aaacccagca ctaaagaata tgctaaagca gcgaaaactc aacctgctct cgaagaagag
     2941 gttaaaaaaa ctgaagcttt tgaatcagta gctcctcctg atacaactga acccgctccg
     3001 gtccctgaac ctaaacaggc tgaaccagta gaacaagcac cttcaaaacc aaaacctgag
     3061 gaaatgctgc ccaaaggaaa accacccact cgtattattg ttccccatca gcaagctaca
     3121 ccggttatta gcaaggggaa gaaaactact cctaaaccta aacccaagcc tgaatccgta
     3181 tcaagagagc gacccaccac ttttaaaaaa tctccaagag aagaaagaaa aaaggaagaa
     3241 ccgcggaaaa aacctccggt tacgactact cctttaccta ttgaaccagt tgaaacagaa
     3301 aaagaacccg agattaaaaa gtttggtaaa gttcgggtta atcatgaaga attaggtgaa
     3361 aaaagtaaac ataaacgagc tatcgttagc tcagttaaga aaagcaagca aaaggaagag
     3421 tttcccattg atgtggatga agcggagatt tcacgcaata taaagaaaat actgcaaaaa
     3481 accaggaaaa agaaatatca taaagaagca caagcaacca gtgaaaacac agaaaataag
     3541 gttattatca gggaatttac ttctgttagt gagctcgcaa aaattcttaa tgttcctccg
     3601 gcagatataa tctccaaatt ctttgctatg ggtcagctgg tcacaatgaa ccagagattg
     3661 gaccgtgatt cacttgaaat gatttgtgat gaattcaatg tggacttcca gtttgaagat
     3721 gaatatggag ttgatattct ggataaagaa aaggaactct atgctgatgc agaatatgaa
     3781 cctcgtcctc cagttgtgac cattatgggt catgtagacc atggtaaaac ttctattctg
     3841 gattatatac gcaataccaa tgtagtggca ggtgaagcag gtggtatcac tcaacatatt
     3901 ggagcttatc aaattgagat taataaaaat aaaattacct tcattgatac tccaggacat
     3961 gaagcattca cggcaatgcg agcacgtggg gcaaatgtaa ctgatatagc tgtcattgtt
     4021 gttgctgcta atgaaggaat gaaacctcaa actaaagaag ccattgacca tgctaaagcg
     4081 gcaggagttc ctatcattgt cgctatcaat aaagtagatt tgcctgatgc taatgtgaat
     4141 agagtcattg cacagcttct ggattatggc gttatgctgg aacaatatgg tggagatgtg
     4201 ccctggtgca ctacttccgt tgtaaccggt gaaggtatcc tgaatctcat agagttgatt
     4261 cttataatgg cagaaatgat ggaactcaaa gccagaaaga aagtgcctgg ttctggtgtg
     4321 gttttggaag ctgaaaaaga cccacgtatg ggttctatcg ctactattct ggtgcaagaa
     4381 ggaacaatta agaagggtga tattattgtt tgtggtccca ctcatggaag aatccgtaga
     4441 atagaaaatg agagaggaca ggaaattaag caactttatc cttctgatgt gggacgcatc
     4501 tttggattga atgatgtacc caaagcagga gacctggtta atcaggttga ttccgaaaaa
     4561 accgctcgtg ctattagtac agaaagaaat caaatccgat tggaacgaga aaaataccaa
     4621 aatcaagcct cactacagaa catctttgaa cgtataaggg atgaacaatt aaaggaacta
     4681 aatcttatca ttaaagcaga tactgacggt tccgtggagg cactggcaga ttcttttgcc
     4741 aagatttctc atccacaagt gattcttaat attattcata aaagtgtcgg tggcatcaat
     4801 gaagccgatg taagtttagc tgctgcttct aatgctgtta tcatcggttt tagagttaga
     4861 gccaatcaac aagctaaaaa gatggcagaa gaagaaaaag ttgatatccg gctttatcag
     4921 gtgatttatg atgctattga agatgtagaa gcctctttag aaggaatgtt acagcccgag
     4981 tataaagaac atattattgg aacggcatcc gtgcgtcagg tgttcaaaat taaaaaagtt
     5041 ggcactgtag caggttgcca gattgaaagc ggttatgtca ggaaaggttg taaggttaga
     5101 gtctatcgta atgatgtgat gattgcagaa gataaccttg ccaccttaaa acactatgcc
     5161 gaagatgtgg aagaagttag agctggcagt gaatgcggaa ttaccttaga gaatttctcc
     5221 gacatcaaag aaggcgatat ccttgaattt tatgtcctgg aagaaagtgc taaaaaataa
     5281 actatgaaat cttataaaat cccccggctt caagaagagt tgcgtaaaat cttgaatata
     5341 actttaacca ccaagattaa cgacccttct ttagcctggg taactattac tgatgtggtg
     5401 ctttcccggg atttaaagta tgcaaaacta tacttttccc attataataa ccccgcttcc
     5461 catgaaaata tcagggagcg tctaataaaa acctctggtt atttcaaaaa acaaattgca
     5521 ggagcacata taatgagaac aatacccgaa ctatctttct attacgatga cacagaagac
     5581 cgggcagctc gtgttgaaac cttactcgca agcctcaaag ataagtataa tgatgatgag
     5641 gaggactacg aaaactttga cgaagatttt gactgggatg aggaatatga ggacgaagat
     5701 gacgacctct ttgaccttga tgaagacgat gacgatattt ttgatgacat aattgatatt
     5761 gatgaagatg aaggggaaaa caaataggtg taatctatag attatctctg attcacctta
     5821 tcttctaact aaattagtac caaattaggc taaaggaggg ttattatatc caatgaaaaa
     5881 ccttaataat attcaagaac taaaagatat ccttaatggc tttctgaatg cttccaagtc
     5941 ggtcgctatt atgacacata ccgaaccaga tggcgatgga ttctgtgcaa gtttggcttt
     6001 acaacgacta ttgagttatc aaggaattga tagtgagatt ataattgatg ctgatagtca
     6061 actggaacgc ttcaaattct taatggaagg agcaaaactg agtatatttg aaagcggaaa
     6121 ttactacgaa actctcatag ttctggattg taatagctac acccttttgg ggaacagaga
     6181 tgaattgctc agaaccgctc gtaaaagatt agttattgac catcatattc ctactgataa
     6241 ggaattagac agcgattaca cttttgtgga tatcaccgaa gtttgtacag gctctatcat
     6301 ttataaaatg tttaaatctg aaattaataa tctgcctcct gcggaacaaa gatttatagc
     6361 taataacttg tatatcaccc ttataaatga taccaataac tttactaatt ccaatacgga
     6421 tgcaaaatcg ttatctcttg cagctgaatt agttgacctc ggtatagtcc cttccgattt
     6481 atataaagct tttttcctta ataataccac cagccaaatg aaatatctgg gagaagtgct
     6541 ttcgggaatg gaactctata atgagggtca gattttattt ttatattctt cactggatat
     6601 gcaaaagcgc aatcatttat ctactaatgc tattagtgtg cctcgttttg tccagggcat
     6661 taaaggtgtg gaagtaattg cttttcttcg tcaggatgat aaagaagcct acaatctcag
     6721 tctccgttca gataccgttg atgttaatgc catagcggtt tcttatgggg gtggtggtca
     6781 tagaagtgct tccggtgcta tgctttatgg ggaactggag gagctaaaat ctgacctgat
     6841 atcaaagctg aaacaggctc taacagaatt cccaaataat gtctgaaaat caaggtttcc
     6901 ttctgattga taaagcttcc ggcagttctt cttttgcctt agtgaaacaa ttgagggctc
     6961 taaccggaat ctccaaaatc ggacataccg gtactttaga tccctttgct tctggattat
     7021 taattttcgc tctgggttcc tacacacgcc tttgccagtt tctggaattg caggacaaag
     7081 aatatgaggc agtacttaag ctgggagaac aaacagaaac cggtgacctt gaaggaaaag
     7141 tgatagcagt aagtgaaaat atacctaacc aattagataa ggaagcactt agagaacaaa
     7201 tattacaaat caaatctctt gccactccag cttattcagc tgtaaaaatt aaaggaaaac
     7261 cagcatattc ctatgcccgt aagggaatta aagtggaatt ggaagaccgc cctgttcata
     7321 tctccaaatt tgaagtgaca gattattctc ctccttattt aagtttttgt tgcaaagtct
     7381 ctaaaggaac ctatatccgt agtctgggag aacttatagc tcaacgctta gaaacagtag
     7441 gacattgtgt taacttgcga agaaccgcta taggtcatct tagtgtaaat caagcctcta
     7501 aaattgaaga tattacaaaa gaaaatttca cttctcattt ctttcccata gaagagctgt
     7561 ttccagactt tgaaaaactc tacttacccg aaaaagagct aattcacctt agtaatggca
     7621 atcccataac caattctggt aaggactacg agaaaatact gatatttgat tctaaaggca
     7681 gcttgcaagg cgtggcaaag agaaaagatg gcataattat tccttatata aatctgaaga
     7741 gttgatatga aaactgtttt aagtatgggc aattttgatg gtctgcatat tggacataaa
     7801 aaacttcttt ccagagttag ggagctggca gaagagcgta atcttaaatc tgtggttatc
     7861 acttatgaca atcatcctgc tcagacgctg aataataatt cctatcccta tatcctgctc
     7921 ccaccagagc ataaaagaac taaaatttta gatttgggta tagacgaggt tcaattgctg
     7981 cattttgatg aaaaactggc aaaaacttca gcagaagatt ttttgcgaga gtatctgatg
     8041 gctaaataca atcctgctat cattgtgctg ggttatgact ctcatttcgg ttatcaaaga
     8101 agaggaaatt accagtttct cctggagcag agtaaaaact atggatttga agtggaatat
     8161 gtagagcccg tcctatataa taatcgcatt gttagcagct cattaatcag agaaatgctc
     8221 atatcaggta atctttatgt ggcaaatcgc ttacttggtt atccttacac cctttatgga
     8281 accgtgggaa ggggaagagg tattggtaag gaactgggat ttccaactgt aaatcttcaa
     8341 ttggaagaca cctgtcagct tgttccttat aatggagttt attttagtat tgcacaaacc
     8401 ggagatttag ttttttatgg attgacaaat ataggcatca gtccaacttt gaaacataag
     8461 gataaagctg agatagaaac ctatctgtta gatataaaaa gagattttaa tgactatact
     8521 tttgccgtat cattgctaca ttatctacga gaggaaagga tgtttcctga taaagatgcc
     8581 ctgataagag ctattgaaaa agatgtcgca ctcgctaaaa aattggtgga taataaaata
     8641 atatgcgatt tctaataact tttgctgtcc tcttatttgt atccttttcc tttgctctgg
     8701 actttgtccc aggttctggt tctcaagtga ttccagagga cagtaaagaa tataattttg
     8761 cctatcatca aggaacggat gattatcact tttatggctc agataagtgg gcggtatttt
     8821 ttgattttgc atctgtatat cctgctgctg aaatctcaca atttcttgtc tctaaagcct
     8881 taatctacct tcctcaaata ggtgattcca ttaaggtgga gctttttacc aatggatata
     8941 atagtcccgg cacactttta acctggaata aggtgctggt ctcttccaac tggttagaaa
     9001 taactttccc tcaaactgtt caagaaagct ctttgtggat ggtagcaaca tattctacca
     9061 gttttattgg aacttttgtt tccgcttcct atggttctgg aacgcatagt tattattcta
     9121 ataccaatgt gccacaatct ccttattatc aaagttttgc aaatgcagga tttagtgccg
     9181 aactactgtt cggactcaca ggagaatttg tgctggattc acaaataatt gacctggaac
     9241 taacctattt taacctggaa ggaatagttg aaccaggaca aacggtagct ccaacctttg
     9301 ctatctataa tcattccagt attcccattt ccgatgcggc tatccattta aatgtttatt
     9361 cgccagaccc agactttgcc ttctttgatg aacttcctgt aacggaaact attgctcctc
     9421 attctcttta tgaatttaat gaccagaatc cagattttct tgagcatcaa ttcacacttc
     9481 ctgaaagacc tttacagtta aaattgagag ctgccttggt tagcagtcat aacgataatg
     9541 accctcaggc aaataatatt aagttaatac atcgttttag ctttgccgat tcttatccta
     9601 ttttcctgat agagaatttc ttacgctggc aaaatgcttc ccagatatcc tggtcgcaag
     9661 accaatataa tcttccgcaa tttcatattt taaactattt tcctatcctg ggtgattctc
     9721 tttccaatct ggcagcgcaa aatagattta attggtatca tttcaattca ttacctcgta
     9781 ctgttatcaa tggaaacctt aaaattaatg gtttctcctc taattatggt aatcagttta
     9841 cccaattatg cactcaagct cagaatagta agactttcat atcttctgct gattgtcgca
     9901 tcattagtga ggaaaatagt gaccaacttt ccgcatcttt aaccctgaat aatgcacata
     9961 ctcaactata ttcttctgcg gtggagtatg atatcgtcaa tctgtcgcgg ctatttgtag
    10021 gtttgttcaa aaaagaaact ttcaataaca gtgaacgctg ggtgattgat cgctggatag
    10081 tgcaaaatca agctttaaat ggaagcctaa atgccgctca aagtctcact ttagattttt
    10141 ctattccttt aaataatatt cctttaaatg aattagaaga atcctaccgt ctttactatt
    10201 ggttgcagat gtcagatacc ggaaaaattc tctattcttc ctttgcggag ttcagcaatt
    10261 tcgttccagt caacgatatt aactatagta tcccccgatt aaatatcact tccaatcctt
    10321 tacgccgggg tgaacagatg aagatatctc tcaataatgg aaagcaatta ggacgagtgc
    10381 aggtctataa tctgaaaggt caaaagatta aagattttta caccactcaa cctgaaattg
    10441 ttttcacatc tgatgaattt cctaccaatg gtatctattt tctccgattt cccgagcttc
    10501 ctgcagctaa agcgggtaat cctaccactc ataaaattat tatcttaaag tgaataacta
    10561 tgtttgaaca cattaatata tccgacctga aagaacatct gggagaagaa gtagtcagtt
    10621 tttatctggt gacttccaaa gaaattcgtc agggaaagaa tgaccgctat ctatatcttc
    10681 gcttgcaaga ccatagtgga agtattaatg cctatgtctg gaaagatttc cagaaagcag
    10741 cggaagagtt ctccgaagga gatgtgataa aaatcaaagc tatggtcaat aattacaaag
    10801 accaggtgca gctgaatgta agtcaattgc gctatgcaga taaatcggaa tataatattg
    10861 aacattttat tagttccagc agtaaacctc cagaatttct ggctgaacag ttcttctctt
    10921 tcgttgataa agtagaaaac ctctggatta ataagctcct acaccggatt tttgatgaca
    10981 aacaattctt caatctcttt cttaatgcac cggcagctaa aagctggcat cacaattatc
    11041 gccatggact tatagaacac accgtcaatg tagctactct gtgtgattcc gtttgcactc
    11101 tttatcctgt gcatagagac ctgattatcg ctggtgcctt gcttcatgat gtgggaaaag
    11161 tatttgaata tagtcctgct cccaatattg attttaccga tttaggtaga ttggtcggac
    11221 atctcagtct gggagaccag tttgtatgtg aaaaagctaa ggaaattata ggtttccccg
    11281 aggaaattct cttgaatctg cgtcatatga tacttgctca tcatggcgaa tacgaaaaag
    11341 cttctgttcg tctacctcaa acccttgaag cccttgttct gcacctttgc gataatcttg
    11401 atgctcaaag tgtcggagtg gcacaacttt tagcggctgc tccagatgat gcagtctgga
    11461 ctgaatacga taaattaaat aatcgttatt atcacctgac taaaatctga ttgatgttcc
    11521 agctcgctgt tttggttagt agttcacagg gaaatgccag cttcatccga acagatgaaa
    11581 ctgccatatt actggatgct ggaacccctg ctaaaactat atttaacgct ctacaagacc
    11641 ttgggattga tagatatgaa ataaaaggag tggtactgag ccatgaacac tccgaccata
    11701 tcaaaggagc tggtgcagtc ctccgttatc ttaaggtgcc tctttatata aatgaagaaa
    11761 cttatcacca atgtcagtat cgtttaggaa atattccagc tggcataaat ttcttccaga
    11821 cgggaattcc tttccgcatt aatgacctcg ttatccatcc tttttcttct tctcatgacg
    11881 ctgtagaaag ttgcaatttt accttccatc gtctacaaaa tcaggaacga aaactgggaa
    11941 tagcaaccga tttgggattt cctactactc tggtagtcaa taaattaaag gattgtacta
    12001 ccatcgttct ggaaagtaat catgatgaaa aaatgctgat ggaaggacct tatccctgga
    12061 gcctaaaaca acgaattaaa agcccgcaag gacatctttc taatcagcaa gcggtgggtc
    12121 tattgagcca aattatgcac tatgggttag aaaaccttat cctggctcat ttaagcgaaa
    12181 caaataataa tcctcaactc gtatgggata cgatgaatga ctttctacaa tccatacgca
    12241 gtaatgttaa cctcctgatt gctgaacctt ataggcatac tccactgata aatatttaag
    12301 aagcataact tttatccctt aattctttaa tatatcctcg gtaaacacat taaactataa
    12361 tttagcctcc ctctctttgc ataagtcaca gtgcttaaat agtgaattac taatggataa
    12421 ataagtgata atgtattata gtatatgcag ttatccatta caaaccattt cctaaaacat
    12481 ttaagtgaag tcataatcac ctatttatct ctttctatac tttaagaaac tcaggttaaa
    12541 aggacagaaa aattaataat tatttgtagg ataatgaaat atagagtttt aaataagggt
    12601 tctgaattaa atgaaataaa gagcgaatag aagaggataa tgcaatagtg ctaacttact
    12661 gttaatcata tagataaatt aaatttttgt gtttttcgga aatttattct aaggatgcag
    12721 ataatatatt gaaaaataat aagatataat tcaaaaataa ttcacgatta ttcttgacaa
    12781 aaaacccata ttttatattc tgacatatag atatgaatat tatttttcta agtatggagc
    12841 accatcctct ggaaaagaaa gataagatta gttaatatcc aaggagacaa tgaattatga
    12901 cagataataa ttatacctct aaaaatatta aagttatgaa ggggttggaa ggtgttcgtg
    12961 ctcgtcctgc tatgtatata ggcagcactg gaatacaggg tctgcaccat ctggtttatg
    13021 aagtagtgga taattccata gatgaggcat tagctggatt ctgtgattat ataaaagtta
    13081 ctataaccac tgaaggcgaa gttgaagtag aagataatgg tcgtggtata ccagtggaaa
    13141 tgcatcaaga gatgaaactt cccgctttac aggtagtgat gacgatgctg catgctgggg
    13201 gtaaatttga ccaggaatcc tataaagttt ctggtggact acatggtgta ggcgtttccg
    13261 tggtagttgc tctttcggaa tatctggaag ttaaggtgca taaagatggt aagatatatt
    13321 atcagcgtta tgaaagaggg aaagcggtta cagatgttat ggaaataggt gaaacagacc
    13381 gcaccggtac tattataaaa ttcaaaccgg atagcactat ctttgagacc acggagttta
    13441 gttttgacta tctaactgct cgtctaaggg aattagcgtt tttgaataaa ggagtgcgca
    13501 ttatccttag agatgacaga actggaagag aacacgattt taaatatgac ggtggaatcg
    13561 ttagttttgt tgaatatctc aatcagaata aaaaacctct cagtcctaag ccactttata
    13621 tagaagcaaa gcgagatgac cttgaatttg agctggctct gcaatacaat gaaggttatc
    13681 aggagaatat tttcagcttt gccaataata tcaacacctt agagggaggc acacatctca
    13741 gtggcttcaa aagtggtctt accagagcag taaataccta cattaagaat gctgacctgc
    13801 taaagaatga gaaggtaagt ccttctggtg atgatattag agaaggatta actgcggtta
    13861 taagcgttaa attaaagaat cctcaatttg aaggtcagac caagactaag ttacaaaatt
    13921 cggatttgga agggattgtc aactctgtgg tatatgagaa attattgacc tattttgaag
    13981 aacatccagc tgaagctaaa gcgattacga tgaaaagtat tatggctgca cgtgctcgtg
    14041 aagcagctcg aaaagcaaga gaattgactc gcaggaaatc tgttctggaa agcagctctc
    14101 ttcctgggaa attagcagat tgcactatca ctgaccccag taaaacggaa ttgtttttag
    14161 tagaaggaga ttccgctggt ggaagtgcaa aacagggaag agaccgttcc tttcaagcta
    14221 tcttaccgct ttgggggaag atgttaaaca cggaaaaagc gagagtggat aaggtcctga
    14281 ataatgataa gatacagccc attattttag ctattggagc aggagtagga caggattttg
    14341 atgtcagtaa aatccgctac ggaaagatta ttattatggc tgatgctgat gttgatggcg
    14401 ctcatatcag tacattatta ctcactttct tctatcgtta tatgcgtcca ctgatagaac
    14461 atggtcatgt ctacatagct aaaccaccac tatttatggt tcggaaaaat aaacaggtaa
    14521 aatatgtttt tactgaagaa gaaagagatg aagctatcaa ggaatttggt gaaaaaggag
    14581 tgtttgtcca gcgatataaa gggctgggag aaatgaatcc tgaacaatta tgggagacca
    14641 ctttaaatcc tgagaataga cttttagttg cagtaaaaat ggatgatgca attgaagcag
    14701 atagaatctt tactattctg atgggagatg aagttgaacc acgccgagat tttatacaga
    14761 ctaatgccag atatgttcag aatctggata tttaattggg agttatagat ggaagataaa
    14821 tcaagaatta ttgaagtaca aatagaagac cagatgcgtc aagcttatct ggattattca
    14881 atgagtgtta tagtgtccag ggcattacca gatattcggg atggattaaa gccttcccaa
    14941 cgacgtatca tatatgctat gagtgaatta aatctggttc ctggcggaca ttttcgtaaa
    15001 tgtgctaaaa tagcaggtga tacctcagga aactatcacc ctcacggtga acaggtggtc
    15061 tatcccactt tagtgcggct tgctcaaccc tggaatatgc gatatccctt gatagaagga
    15121 cagggcaatt tcggttctat agatggagac ccacctgcag caatgcgata cacagaagca
    15181 agattgcaaa aagttacaat ggaactattg gctgaattgg ataaagatac tgtcaatttc
    15241 cgtagtaact atgatgaaac acggaaagaa ccagaggtat ttcctgctcg gatacctaat
    15301 ctgctaatta atggctcatc tggtattgca gtaggtatgg caactaatat gccaccacat
    15361 aacttaacag aaatttgtaa tgccgtgatt gcacttattg ataatccaga tttagagata
    15421 tgggaactac ataaatatat taaaggtcca gatttcccta tgggaggata tatccttggc
    15481 acaaaaggaa taatggatta tttcttaact ggtagagggc aattgctctt aagaggtgaa
    15541 gctgaaattg aaactaataa aaatgaacag gaacagatta ttattcgttc tatcccttac
    15601 cagataacta agactgccct tattgataag attgtagaac tggtaaaaga taaaagaatt
    15661 gaaggaataa gcgatattcg tgatgaatct ggcagagatg gaatgcgttt agttattatg
    15721 gttaagcgca atgcggatgc tcaaaccgtc cttaatcaat tatacaaata cactcaatta
    15781 cagaccactt ttggagtgat taatctctgt ctggttaatg gtgttcctca agtggtcaat
    15841 atgaaggata tgctcaataa cttcatagaa tttcgtcatg aagtgatttt gcgtcgcacc
    15901 aattttgaac taagaaatgc ggaagaaaga ttgcatatcc tggaaggttt aagaattgca
    15961 ctggaccatc tggatgaagt tattgccact atccgtgcat cacaaacacc acctgaggct
    16021 agtgaacaat tacaagagaa atttggctta tctgaaattc aagccaaagc tattctggat
    16081 atgagattgc aaagattaac aggtctggaa agagaaaaga ttgaaactga atatgaagag
    16141 ctactgaaag taatagacca tctgaaagat ttattggaac ataaagaaaa gagaatgcaa
    16201 ctcataaaag aagaaactgc tgaaataaga gataaatatg gagacccgcg tcgcactcaa
    16261 attctggaaa gctacaacgg tatcttaaat ccagaagata tgatagctga tgaactggtg
    16321 gtcgtaacca ttactcacga aggctatgta aaacgtatgc ccatagatac atatagagtc
    16381 cagggcagag ggggaagagg tttaaccgct gctaatctga gagaagagga ctttattcag
    16441 tatatctttg tggcttctac ccattcttat atcctgctct tcacggattt aggtaaatgt
    16501 tactggctaa aagtgtatga gattcctgaa gctggaagaa cagctcgggg taaagctatt
    16561 gttaatttgg ttaatctgca ggataaagaa aaagttaagg cttttgtcac tctcaaggat
    16621 ttcaatccgg aagaaaatat cgtgatgtgc accaagaatg gtatggtcaa gaaaactccc
    16681 ctggcagctt tcagtcatcc ccgcagttct ggaattctgg ctataaaatt gttaccagga
    16741 gatgaattaa tagatgccag aataaccgaa ggcaatgatg acctcatttt agccacacat
    16801 aatggatatt gcaatcgttt tagtgagaga gagataagac ctactgctcg tttcactcaa
    16861 ggtgttagag gcataagatt gcgtgaagaa gattatgttg tttctatggc agtaatatct
    16921 caggatttat tgatggaaaa tggaaatgcg ctaactaaga ctattttagc gattagtgaa
    16981 aatggctatg gaaaacgcac tcggatatct gcctattcta taacaagaag aggaagcaaa
    17041 ggggttatta ctcttaagac aaatgagaga aatggacatc tcgcagctct aatgctggtg
    17101 gacgaaaacg atgatttggt aataatcacc caggaaggaa tgataatccg tcagaaagtg
    17161 agtgatatct ctatctttga aagaaacact caaggtgtgc atttgattaa tcttcgggaa
    17221 aatgataaag tggtagatat caccataatt cctaaggaac ctaatgaaga agaactggat
    17281 agagaagtgg aaaaggcaaa aaatgcccct tctatgcaag catctaatac aatggaagat
    17341 gttacggaag aacaagatga agaagaagaa ttcagagatg aagagcttcc tgaagaagaa
    17401 tgatttaata tgcccccttc aaaaatgcaa gggggcttct tattttaggg taataagttg
    17461 ctggtttatt ttcgtcatct ctatgctact tctatctgga tgtgcagtaa aaccatatgt
    17521 tccggtccca ggcgatttct atacccaaga agcagattgg gcaattatca ataccgatag
    17581 cctgaatatt tttgtccatc ctcaatctta ttacggtgtt gcccagaata ctgcctccag
    17641 ctattttact ctctatgtgc gagtgaaaaa taaatctaaa aagaatatct ctttaaatcg
    17701 ttccagtttt agcattctgg caggaaatca acagtttgat tatatccctc tggaaatcat
    17761 cttaggcagc ttgcaaaatt atagtctgct gcgttattac caggaccctt ttgcttcacc
    17821 tattccagct aattatgata aatacttaca agatattcag caacaatacc ttgaactggt
    17881 taattcctac ttcagttttg gtgaactact tcccggtggg agtaaggaag gttatctttt
    17941 ctatgataaa aagatagaac aatacaatag ttttgaatta gatgtttggg gaacaaagat
    18001 aaaattcgta agaaaatagc aaataaatcc taaaatatat cttccacccg agcttacatt
    18061 atcttctaac caaataacta ataaaccgag gggttattct cctgcttctt taataaattc
    18121 gcataaaatt taataaattc gtagcctgtt ttggacatat atagcactta tctacttcat
    18181 tcatatatat ttataacatt tatgcctcac ccttataccc cccttatcta tcacttatca
    18241 tttta
//
LOCUS       DAORZF010000036        16805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_2783_length_16805_cov_337.565493, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000036 DAORZF010000000
VERSION     DAORZF010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 16805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16805
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2783_length_16805_cov_337.565493"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     repeat_region   1..264
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1..37
                     /rpt_unit_seq="gtaagaattgaattgacccgtttgaggggattgagac"
     gene            627..1067
                     /locus_tag="PK015_05265"
     CDS             627..1067
                     /locus_tag="PK015_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33910.1"
                     /translation="MKVKFKYGIRTYSGKLDDLVFCSCYNDTVCIARKYVYPRFSEQI
                     VSISHNLATIWENGSSEFKAQLELYAKLNETENVPDDQWAPNSYALWIEVMYNWAADN
                     NVDLDSLTIEEFTIMGTAVRTVKNCVLNGYLKPVTGYDMMTAPF"
     gene            complement(1523..2776)
                     /locus_tag="PK015_05270"
     CDS             complement(1523..2776)
                     /locus_tag="PK015_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOL33911.1"
                     /translation="MPNFAYIIKDAKGARVEGIVKADSLDQAVEKLSKEGSTIISVKA
                     AAEGAFKGNLSLADRIMLSIYKWRTGVSLKILVFFTRQLATMYSAGLTIEKALVDLEK
                     SEKNKKFAKVLKQLADDIRKGYSLSEAMEQHPGVFNPMYVALVKAGEVSGTLHTTLNE
                     LADYLEKMEDTHRKVRSAMAYPVFILIFLIIVVFFLFYYVVPMFAEVYAGFNAELPGP
                     TQVAIAISNFLTDNIFLAILVILAIAATFWIVNLTDRGRYVWDSIKLKIPIFGSITLN
                     SIMSKFARTFSILMAAGVPIMDTMELTENVVQNAVIEGGIRRARVMVKEGYGVANAFR
                     RTGLFPPTILQMISTGEETGDMDKLLGKAAEFYEKLVDSVIDRLTSLIEPLLIVIMAA
                     VVGSIIVTVYLPIFSLGEAMSQGLR"
     gene            complement(2800..>3324)
                     /locus_tag="PK015_05275"
     CDS             complement(2800..>3324)
                     /locus_tag="PK015_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOL33912.1"
                     /translation="GEIRDEETADIAIKFSLTGHLVFTTLHANDAPSTITRLIDIGIK
                     PYLVGSSLCLVMAQRLVRKICPHCISDYHPTEQELKDCGITPEEAALINFKIGRGCVH
                     CDNTGYSGRTGIFELLTINPEIRRIIYEGGNQDLIEEAALRNGMRSLHDAAIIKMKEG
                     ITTIREVIKMTIVE"
     assembly_gap    3325..3424
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3425..3780)
                     /locus_tag="PK015_05280"
     CDS             complement(<3425..3780)
                     /locus_tag="PK015_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilB"
                     /protein_id="HOL33913.1"
                     /translation="MAFNPQFARLGEILVHEGYVTEDQVKEALIKQSNFGLKLGETMI
                     KLGYLTEEQLLKALNMQLGYDIVDEKELMDLDIEVVRRIPEPYAVENRVIALREDADG
                     MVVAMADPDNLTVLDS"
     gene            complement(3880..6135)
                     /locus_tag="PK015_05285"
     CDS             complement(3880..6135)
                     /locus_tag="PK015_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33914.1"
                     /translation="MRKLNNLFPIVIFLVLALFLVITSCDRRNPPPTGIGIVKPSEVV
                     KITKIVAEPDTIYCDNNITFSQISVTVKDGDGFAIPGKIVNFRANIGRILRSVPTDSS
                     GVAKTTFWDDGEQGLATIMAVVQNISETTNTVISADTARVEVFIDEVPPVESVTLNFS
                     SQANPFPMTVMQTTEINATAKNILGQTVPDNTLITFSCNKGKFVDEGGNNLGMSIVSK
                     TLNGRAYARYNAGAVATTTPGIEPAYVTASIGDISSTREVMIYPGNPFNIRLQSFVQV
                     GDQMIETDTSSVASPNNIYMLATLNDPHGNPCPTKPVRFETNLGSFLNTTQSITLNTN
                     TMGEAQVRFTPGLAAGAATIKAFANNDTLQTQLIFNIKSDDIYSISFTQQEQINLNVA
                     NTGGTDSAILRVKLKDINGNLIDYPQEVYFRIVNLNPPAGANLNNHPVTDSVLVISTG
                     GEAQVSVNSGSESGILIIRASCIAKDGRWIHATKANIVIHAGPPAEVKPFIGGFNTGQ
                     NMGGGLWRVVAGAVVKDRYGNPVETGTSVFFELINNVTNCQIGAAGYVGNTSVEDDSL
                     AGVAYTTVTYSGNFTYDWITIKASTGAVAGEGVDGYATVQLPLNDPRLEAQVVPEHLN
                     FFSSDTWKSADFYAVLTDGQGKSVGNAVIICVSTKGQFVAIPGFHNNYPYDPPWQIIT
                     DDGSYGIGDKAGWAWGQIKYHKLEIPVGDGVSETPGQTTGVITLRILGTNVSVETSIV
                     LYRYWDGCPPF"
     gene            complement(6137..7624)
                     /locus_tag="PK015_05290"
     CDS             complement(6137..7624)
                     /locus_tag="PK015_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33915.1"
                     /translation="MKKYNQAGVNMIHHPLSKHFILCALVMILLCSVFSIGAQTSNLR
                     KPVTLNCMDEPISSVLNTLSRLSGTNIVLATDQTGMAEKEQKRVTLNLREVPIETAVS
                     LVAKSAGLSYKIYGDNTFLVGTLQNISESAGEQSHIIYLNYLDAEKVSKALENTQVSV
                     VPVEGQNAIMVYTNTDTFEGINNLVKSMDVPQKQIEIRVRLIEINLTDSKKYGIDWSR
                     LNHLTTIIAEDPVNSVGTGLPYNYTDVEGYLPHGNPTDFERLPDQQYFQRINGFHDIG
                     HFSRQLTAFDITIDWLLENNAAKLLTDTRVTALNGNEANIHIGEVVPYVVTDNEKQIQ
                     VEREQVGIMLTVIPTVNDEGLITAQISPEVSSVTELVGGYVPRTRVRRINSTVTVPNE
                     HKIIVGGLLSSNITNRVSKVPLLGDLPFIGKLFQHKTEQIDNSDLIIEITPRIITADQ
                     YRPDPNVQELKSLGEPKLDERMTRRLIQYENVNNDNNNNEENKGR"
     gene            complement(7627..8172)
                     /locus_tag="PK015_05295"
     CDS             complement(7627..8172)
                     /locus_tag="PK015_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33916.1"
                     /translation="MQFRYLKDLAMALIAISLIACAIRLISLNEANRKIPPQSKYTHE
                     SVSDTLRAQIMSIESSILDRKNFVFSVTHDPLRQGNIIKDRFDMQKEFENAVRNMFRP
                     TGTYIDEETGRRLVTIEYQDRIYTAGVGDVVEGRKIMWITEQNIGIYYGGPQTIPIQP
                     RPPIPDFSLEETRQKSLEQNY"
     gene            complement(8174..8719)
                     /locus_tag="PK015_05300"
     CDS             complement(8174..8719)
                     /locus_tag="PK015_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33917.1"
                     /translation="MKEKYLLFIVGMILVTILFFWVANTNLKKNITEIERYDKRIKTS
                     LEKLNSAMLMDAQLSQFKEILDNTLTTNERFSIDELNDFRREIERLRDNNKLKLIRIS
                     DTNKYNEPGLLEHTYSLELEGTFQQMGKFISDLEAQNYIIKIQYLDVSPTQVSGKTDP
                     NAPNTYKITMELSIYKVKKEA"
     gene            complement(8716..9321)
                     /locus_tag="PK015_05305"
     CDS             complement(8716..9321)
                     /locus_tag="PK015_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33918.1"
                     /translation="MENIFYFKINLNKYGELKLQVEKENKTFRNAVIIYCICAALLFV
                     GLFYINKDMKKKVDNRRKFYNQISSQLAAVESSGEYLSAKDLEKLAKTFNNRIFWTKK
                     LEALSREIDKQLAIRKFTYSNGVLTLNGIIEISKNIQEGDVIFNFIQRLKNNPEISND
                     FPEIKAGPQMRQISKDTEILEFIIECYSREAAPLQPQKVQL"
     gene            complement(9331..10401)
                     /gene="pilM"
                     /locus_tag="PK015_05310"
     CDS             complement(9331..10401)
                     /gene="pilM"
                     /locus_tag="PK015_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HOL33919.1"
                     /translation="MKKTKAIKFKESVGIDIGSHSVKVVHLKRLHEGFKLLNYEIRPT
                     IPIDVEYVPSDLRPDRFAPVIVEILKTLHINPKKIQHLVTSVGGDNTSIKQIKTIFLP
                     DDELESALFFEAKKHIPISGTDMVLDYQVISVEEKTNNMNILLAATSKELLNEHTNIL
                     TTAGLTPNIVDIDSLAVANSFILNAFVEEGVYILLNLGAHRTNMVIWGPEAKFFARDI
                     PYGGYNFTRDIMRKRQMEWPEAENYKMEWGLLDDPSRAGTQTISMLDISEKATEDAIV
                     EELRRSLRFYVKEAGNSDFRKVYLMGGTAKLRGLPEYIQEKVNIPTDVFHPFINVEMP
                     EKFEDKKDPQLALAIGLAMRLE"
     gene            complement(10429..12306)
                     /locus_tag="PK015_05315"
     CDS             complement(10429..12306)
                     /locus_tag="PK015_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33920.1"
                     /translation="MFNIFKHPSGNIAIALLLSVIAVMSGFSLSSMAMNDVVAFQYDF
                     ENFQSTLFLRSEAYRGQKIAQKLGTILIPVRTSERSIELTNSALKKTFKIQSLLSKGR
                     LLTSQEVVMGDQKQQTIVESLVKSKTGIGQVAAMNSKFSMIRKYGIYTLETETFAKFM
                     YFTDTDESPNGTNVYFHGPDMLYGRVHSNTDIWIKQTYGGVNGGWPIFYGWVSTAGEI
                     QSHSGSYSESQVFPGGLSEGYDYVVFPENATLIRRNGQKVGPLNYNPDVIVYVEVDGA
                     SFTYYKGDIQSPRLESADVYNPYPPPTATEPLFTNTYQVRDTVWTFGAHGTSNNRSNW
                     VNNRLWIRGTFATYQTWGCADTMFLVDDILLENTPIGSDPANNTTDVVGLVSEKSIVI
                     KYGYRSPVDTLRHHDTCGPDTGYGGIWIYAAMAALGDGHGNTHQDGVFTFEYQHPHPS
                     VPDYRYNNVLYTKIDLHRYHFPQSNAYKWATCNGPRNLKIDYPWYNPLWPERTPYLER
                     GYINIYGSVSQRRRGFVHRSYYDEEWPSNDEWNQPIDFCGPTSAPTAVQHNDPVFGFQ
                     LTNVNYPGTSGSGTGYEKNYHYDNRFYHTSPIDFPQVNRKDETPFAAVNWIIKRPPEY
                     I"
     gene            complement(12336..12935)
                     /locus_tag="PK015_05320"
     CDS             complement(12336..12935)
                     /locus_tag="PK015_05320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOL33921.1"
                     /translation="MKKHKSSILARQNGVTLIELIVVMAISVLLLLISGLGIGTFFRT
                     YKELTAWTKLQQDALNCLNLIKNGVPVGTGTDTEYIGVINAMTLELTNTTTNVSTGLK
                     VIPPSASDIYKFDNAHFYLYNGAVRCTYVHHGVQVASPIYIFPKEEDLDDIIIDKFLF
                     TKVNPEPNVYVVQVELNARIKTGADRYEMVKFKTKMAKK"
     gene            complement(12941..13396)
                     /locus_tag="PK015_05325"
     CDS             complement(12941..13396)
                     /locus_tag="PK015_05325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOL33922.1"
                     /translation="MNSRNSAHSFSATKLLQEQKGMTLLEVLAVMVIIVVMVLAIYIG
                     IVYAEKQLLINYRDRVATLLVTGELEMEYYRHVHDKPFELQVNREYIIDDRDPNHILV
                     GYMTIEQKTAQESSNEQLLTYIYLEATLRWIDPDTKKERFIRMREDYFI"
     gene            complement(13396..14454)
                     /locus_tag="PK015_05330"
     CDS             complement(13396..14454)
                     /locus_tag="PK015_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOL33923.1"
                     /translation="MTIYELLQFTAEAGASDLHIAAGAHPMVRVNGKMKRLNLPILSP
                     EEVESLVFGVMNEVQQDMFKEQLEIDFSTKLSNDVRFRVNAFHQINGISCAFRVIPNE
                     IKSYEELHLPDILKRLTQKEKGFVLVTGPTGSGKSTTLATMIDSINESRYCHIITIED
                     PIEFVHQSKNSLINQRELGHDTWSFTAALRSALREDPDVILVGEMRDLETVSLALTAA
                     ETGHLVFATLHTGSCTKSIDRIIDMFPKEQQQQVRSMLSESLEAVISQTLLPTKDGTG
                     RVPAVEIMIANTAVRNLIREEKTYQIPSVIQASTKEGMQTRDQSLYNLVMNNLIERTV
                     AEQFADNPKQFATGAGFV"
     gene            complement(14472..14918)
                     /locus_tag="PK015_05335"
     CDS             complement(14472..14918)
                     /locus_tag="PK015_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOL33924.1"
                     /translation="MLNKLQGQKGFTLIEVLVVVIIVAILAALAVPRYLRYVEKSRST
                     EAQTAIAAIRKTYDVYRQTTGTTQDYTIEKAMKDANLGESTLKNWKFEVVGNPPKKFI
                     ATSTEEFPGGPDKQVWYDVQEAKFHGYGIDEFTNPMTEAMETETGK"
     gene            15090..>16805
                     /locus_tag="PK015_05340"
     CDS             15090..>16805
                     /locus_tag="PK015_05340"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOL33925.1"
                     /translation="MHFKKWFIVTLVSLIAITTLCADNLAPVEVSKQNRINETDILAE
                     FDGGVITRADLEKRISMLPPNVQGRYKTMEGQKQVLDAIAMEEVFAAKAMQLKLNEDP
                     EVLANIEATKKQYLIQEFYKRNITDRVAVTEEDKQRYYEENKKLFYVNPNITANIIQL
                     PDEETALQAIQELRSGKSFEEVSNLYNINNYIKSLKGVVKNIRLNGNIPGIGNDYELE
                     DLIAKAQPDTTAILGPYKTTTGWYVFRVIDYQPGYQKTYEDVYSELEQRAKPEVENRL
                     LNALMSDLKTKYAVVVDSTLVNQIDLRNPSKNKDIEEKVLVTASEPELTITVKSLLDS
                     FSKLSPQEQLFFTKGGGANQFLEQELIRNLLYLEAKNQNYEEYLKDNENYQQMQRYYM
                     LQKAYKILVVDSIKIDSEDTRAYYDAHINDFTTPGYRTIKVLWFDDEKTAESVRKKYA
                     LYTAFDDTTRINKLIEKESKKPNLSVLDHIYNNGIITGIGPDKDFCDMVWNNPIGYIS
                     PVFKTARGDIVFFQIIAETPAQVKSFTEVEPSIYNILRRDRQMAQQEKVTRELYEEFH
                     MVLYPE"
BASE COUNT         5189 a         3160 c         3010 g         5346 t
ORIGIN      
        1 gtaagaattg aattgacccg tttgagggga ttgagaccat ctctttcatt cttgagacat
       61 tctcttacga aattcgtaag aattgaattg acccgtttga ggggattgag accttcacta
      121 tcctcaagac tccacagttc caatcttgta gcttgtaaga attgaattga cccgtttgag
      181 gggattgaga ctaaagatag actctcagct aacgatacta attcactgta agaattgaat
      241 tgacccgttt gaggggattg atagtattac tagatgcaaa ggaggacctt tgcataccat
      301 tttttttgca taccaatttt ttgcatacca atcgggtgag aatattaatg tagtaaaaaa
      361 actatacaac gacaaaacag ctgttattta caccgaatag ctcataattt cctaagttta
      421 ttgtttcaga aaagttaatt ataatgattt cgtacaactt tttactgtta tttacagttt
      481 aggggtctat ttatcctcca agatatcctt ttctaaatga atttgagata ttaaaaccaa
      541 tctataaatg tcatccctat atatatggag ggacgaaaaa aatataaaag gttccttcta
      601 atatttgatt tattatggag gacaaaatga aagtcaaatt caaatacggt atacgtacct
      661 acagcggtaa acttgatgac ctggtatttt gtagttgtta taatgacacc gtgtgtatag
      721 cgagaaaata tgtttatccc agattttctg agcagattgt ttctataagt cataacttag
      781 cgacaatatg ggaaaatggt tccagtgaat tcaaggcaca gctggaatta tatgcaaagt
      841 tgaatgagac agaaaatgtt ccagatgacc aatgggcacc taattcctat gcattatgga
      901 tagaggttat gtataactgg gcagctgata ataatgttga ccttgatagt ttaacaatag
      961 aggaatttac gattatggga acagctgtga gaactgtaaa gaattgcgtg ttgaatgggt
     1021 atttgaaacc agtgactggt tacgatatga tgactgctcc attttaattc ggagggcaaa
     1081 aaaagcaggg gacagggata gtaacttgtc ccttagctgt taatttaact tataataata
     1141 gaagcttaaa tgaaggagaa tatgctcctc aaaatagcag ataaaatcaa tgggggtatg
     1201 gaataattac gaattttgtt atctatatga gataagaata gaatgctttc tccttttggg
     1261 aaatccctat atgagagggg aatttaattc ggtatcagca ttaatctttg tcatttatta
     1321 tcctctactc gctaaacata aggaggttgt gccattctta aaatgacaag gatggcacag
     1381 ctatttcatc ttttggggag tatgtttttg ttagatagag ggaatggtta agtgttaaga
     1441 aactaattta atagtaatta aatataaacg gggaaaggta aaaccaatcc ccgattatgt
     1501 atttatgagt taacctttaa tattatctaa gaccctgact catagcttca cccagactaa
     1561 agataggtag atatacagta acgattatac ttcccacgac cgcagccata atcacaatca
     1621 ataatggttc aataagtgaa gtcaaacggt ctataacaga atccacaagt ttttcataga
     1681 attctgctgc ctttccaagt aatttatcca tatcacctgt ttcttcgccg gtgctaatca
     1741 tctgaagaat agtgggagga aaaagacctg ttcttctaaa ggcattagcc actccgtaac
     1801 cttctttaac cataactcgt gcgcgcctga taccaccttc aataactgca ttttggacca
     1861 cattctcagt caattccata gtgtccataa taggcacacc tgcagccata agtatagaaa
     1921 aagtgcgagc gaatttactc ataatggaat tcagagtgat actaccaaag atggggattt
     1981 ttaattttat agaatcccag acataacgtc cacggtcagt taaattaaca atccagaaag
     2041 tagctgctat agccaggata acaagaatag cgaggaaaat attgtcagtg aggaaattac
     2101 ttatagcgat ggcaacctga gtgggacctg gtaattcagc attaaaccct gcataaactt
     2161 cggcgaacat aggcacgaca taatagaaaa ggaagaaaac aacaattata aggaagataa
     2221 ggataaatac aggataagcc atagcagatc taactttacg atgtgtatct tccatttttt
     2281 ccagatagtc agccaattca tttagcgtgg tgtgcagagt acccgaaact tcaccagctt
     2341 tgactaatgc tacatacata ggattaaaaa cacctggatg ttgttccata gcttccgata
     2401 gagaataacc tttccgaatg tcgtcagcga gttgttttaa taccttggcg aattttttat
     2461 tcttttcgga tttttccagg tcaacgagag ctttttcaat ggttaatcct gcagaataca
     2521 tagtagcaag ttgtcgggtg aagaaaacga ggatttttaa actaacgcca gtgcgccatt
     2581 tatagataga aagcattatc ctatcagcga gagatagatt acctttgaaa gcaccttcag
     2641 ctgcggcttt aacggatata atagtgctgc cttctttaga taatttttcc accgcttggt
     2701 caagagaatc agccttgacg ataccttcta ccctggcacc tttagcgtct ttaattatat
     2761 aggcaaaatt aggcataggg aaaaccttct ttccagtaat tattcaacta tagtcatttt
     2821 tatgacttca cggatggtag taataccttc tttcatttta attattgctg catcatgtaa
     2881 tgaacgcatt ccatttctta acgcagcttc ctctattaaa tcctgattgc ctccctcata
     2941 gataatgcga cggatttcag gattgatagt taacaattca aagataccag tacggccaga
     3001 ataaccagtg ttatcacaat ggacacaacc acgaccaatt ttaaagttaa tcagagcagc
     3061 ttcttcagga gttataccgc aatctttaag ttcctgttct gtgggatgat agtcagatat
     3121 acaatgaggg catatcttcc tgaccaatct ttgagccata accagacaga gagaagaacc
     3181 aaccagatag ggtttaattc caatatcaat aagacgagta atagtggaag gagcatcatt
     3241 agcatggagt gtggtgaaaa caaggtgtcc tgtaagtgag aatttaatag caatatcagc
     3301 agtttcctca tcacgaattt caccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnaagcta tccaatacgg tgagattatc cgggtcagcc atagcaacaa ccataccatc
     3481 agcatcttca cgaagagcga taacccgatt ttccacggca taaggttcag gaattctacg
     3541 caccacttct atatccaggt ccataagctc tttttcatca acaatatcat aacctaactg
     3601 catattaagg gctttgagaa gttgctcctc ggtcaaataa ccgagtttta tcattgtttc
     3661 gcccagtttt aagccaaagt tagattgctt gataagagct tctttaacct gatcttctgt
     3721 aacataacct tcatggacga ggatctcacc caatctggca aattgagggt taaaagccat
     3781 ttattaactt cctttataat taaaattcat tatcctgaaa ggatgtgaaa tttatactga
     3841 gggatggctg ttatatgcgc catccctcaa acctttatgt tagaatggag gacatccatc
     3901 ccagtaacga tagagaacga tactagtttc aacagagaca tttgtgccta agatacgtag
     3961 agtaattaca cctgttgttt gtcctggcgt ttcacttacc ccgtcaccta ctgggatttc
     4021 taatttgtgg tatttaatct gtccccaagc ccatccagct ttatctccta ttccatagct
     4081 tccatcatca gttataattt gccagggtgg atcgtaaggg taattattat gaaatcctgg
     4141 tatggcaaca aattgtcctt tggtagaaac gcatataatc actgcattac ctactgactt
     4201 accctgtcca tcagtaagca cagcgtaaaa atcagctgat ttccaggtgt cagaagaaaa
     4261 gaaatttagg tgctctggta caacctgagc ttctaatcgt ggatcattta aaggcaactg
     4321 gaccgtggca tagccatcaa caccttctcc agctacagca ccggtacttg cctttatagt
     4381 aatccagtca taagtgaaat ttcctgaata agtaactgtg gtgtaagcaa cacctgcaag
     4441 ggagtcatct tctacgcttg tatttccaac ataaccagcc gcaccaatct gacaattcgt
     4501 cacattattt attaactcaa aaaagacaga ggtgccggtt tctaccggat tgccatatct
     4561 atccttaact actgcaccag ctactactct ccagaggcct ccacccatat tctgtcctgt
     4621 attaaaacca ccgataaatg gtttcacttc tgcgggtggt ccagcatgaa tgacaatatt
     4681 agctttagtg gcatgtatcc atcgtccatc tttagcaata caggacgcac gtattataag
     4741 aattcccgat tcactaccgc tatttactga gacttgtgcc tctcctcctg tagatataac
     4801 taatacactg tccgtgacag gatgattgtt taaattagct ccagcaggtg gatttaaatt
     4861 aacgatacgg aaatatacct cttgaggata atcaattaaa ttgccattaa tatcctttag
     4921 tttaactctt aaaatagctg aatcagtacc accggtattg gcaacattaa gatttatctg
     4981 ttcctgttga gtgaatgata tggaataaat atcatcagat ttaatattaa aaataagctg
     5041 agtctgtagg gtatcattat tagcaaatgc tttaatagta gctgctcctg cagcaagacc
     5101 aggggtaaat ctgacctgag cttcacccat agtattagta tttagcgtaa tagattgagt
     5161 ggtattcaag aaagaaccaa gattggtttc aaagcggaca ggttttgtag gacaggggtt
     5221 cccatgggga tcattgagag tagcaagcat ataaatgttg tttggactgg cgaccgaact
     5281 ggtatctgtt tctatcatct gatctcccac ttggacaaaa gattgtaaac ggatattaaa
     5341 aggattgcca gggtaaatca tcacttcacg agtgctggat atgtctccaa tacttgcagt
     5401 tacatatgcg ggttcaattc ctggcgtcgt tgtagccaca gctccagcat tataacgagc
     5461 ataagctcta ccgtttaagg tctttgaaac gatactcata cctaaattat ttccaccctc
     5521 atctacaaat tttcctttat tgcaactaaa ggttatgagg gtgttatcag ggaccgtctg
     5581 tcccagaata tttttagcgg tagcattaat ctccgtggtc tgcataacag tcataggaaa
     5641 tggattagcc tgtgatgaga aattcagagt aacggattcc acgggtggaa cttcatcaat
     5701 aaatacttct actctggcgg tatcagctga gattactgta ttagttgttt ccgatatatt
     5761 ctggactaca gccataatag tggctaatcc ttgctctccg tcatcccaga aagtggtttt
     5821 tgcgacacca ctactatctg taggaacact acggagtatt cttccaatat tggctcggaa
     5881 attaactatt ttgcctggta tggcaaaacc atcaccatct ttgacagtga cacttatctg
     5941 agaaaaggtt atgttattat cacagtatat ggtatctggt tcagctacaa ttttagtaat
     6001 ttttactact tcagaaggtt tgactattcc aattcctgtg ggtggtggat tgcgtctatc
     6061 acaagaagta ataactaaga aaagagccaa aaccaggaaa ataactattg ggaataagtt
     6121 atttaacttt ctcattttat ctccccttat tttcctcatt attattatta tcattgttta
     6181 cattttcata ctggattaga cgacgagtca ttctctcatc cagtttaggt tcaccgagac
     6241 tcttaagttc ctgaacatta gggtcaggac gatattggtc cgcagtaatt atacggggtg
     6301 tgatttcaat aattaaatcg gagttatcaa tttgttccgt tttatgctga aagagtttcc
     6361 caataaaagg aagatctcca agtaggggaa ctttagatac tctattggta atgttagaac
     6421 tgagtaaacc tccgactata attttatgtt catttggaac ggttacagtg gaattgatac
     6481 gacggactct ggtgcgagga acataaccac caactaattc agtgacagaa cttacttctg
     6541 gtgaaatttg tgcagtaatg agtccttcat cattaactgt aggaataaca gttagcataa
     6601 taccaacctg ttcacgctcg acctggattt gtttttcatt atcagttaca acatagggga
     6661 ccacttcacc gatatggata ttagcttcgt taccattcag ggcagtcact cgtgtatcag
     6721 ttaataattt tgccgcatta ttttccagta gccaatctat ggtgatatca aaagcggtta
     6781 attggcgact gaaatgaccg atatcatgga atccattaat tctctggaaa tactgttgat
     6841 caggtaaccg ttcaaaatca gtaggattac catggggtag ataaccttca acatcagtat
     6901 aattataagg tagaccagta cccacagaat tgactgggtc ttcagcaatg attgtagtta
     6961 aatgatttag tctgctccaa tcaataccat attttttaga atcagtaaga ttaatttcaa
     7021 ttaaccggac cctaatctca atttgttttt gagggacatc catagattta actaaattat
     7081 taataccttc aaaggtatcg gtattagtgt ataccataat agcattttga ccttccactg
     7141 gcaccacaga gacttgagta ttttctaatg ctttactaac tttttcagca tcaagataat
     7201 ttaaatagat aatgtggctt tgttcaccag cagattcaga aatattctgt agggttccca
     7261 ccagaaaagt attatcgcca taaattttat aggataatcc tgctgattta gctacgaggg
     7321 atacagctgt ttcaatgggg acttctctga gattaagagt aactcttttt tgctcttttt
     7381 ctgccatacc agtttgatct gtagccagca caatattagt gccactaagt cttgaaaggg
     7441 tatttagaac agaagatatg ggttcatcca tacaatttag agttacgggt ttacgtaagt
     7501 tacttgtttg tgctccaata ctgaatacag aacatagtaa tatcattacc agggcacaaa
     7561 gtataaagtg tttggacagg ggatggtgta tcatattaac tccagcttgg ttgtattttt
     7621 tcatagttag taattttgtt ccagggattt ttgtctggtt tcttctaagg aaaaatcagg
     7681 tattggagga cgaggttgaa taggaatagt ttgaggacca ccataatata tgccaatatt
     7741 ttgttcggtt atccacataa tttttcgtcc ttctactaca tcaccaacac cagcagtata
     7801 aattctatcc tgatactcaa tggtaactaa tcttcttccg gtttcctcat caatataagt
     7861 acctgtggga cggaacatat tccttaccgc attttcaaat tctttttgca tatcaaatct
     7921 atctttaatg atattaccct gtcgtagagg gtcatgagtt actgaaaaca cgaaattttt
     7981 tctatctaag atagaagatt caatgctcat aatttgagct ctgagagtat cagaaacgga
     8041 ttcatgcgta tatttagact gaggaggaat cttacgattt gcttcattca aggagatgag
     8101 cctaatagcg caagcaatga gagatatggc aataagagcc atagctaagt ctttcagata
     8161 tctaaactgc actttaagcc tcctttttaa ccttataaat agaaagctcc atagttattt
     8221 tataagtatt tggagcattt gggtcagttt ttcctgaaac ctgagtggga ctcacatcaa
     8281 gatactggat tttaatgata taattctgag cttccaaatc ggagataaat tttcccattt
     8341 gctgaaatgt tccctctaat tcaagggaat aggtatgttc taataagcca ggttcgttgt
     8401 atttattagt atctgatatc ctgattaatt tcaatttgtt attatctctt aacctttcaa
     8461 tctctctccg gaaatcattc agttcatcta tagaaaaacg ctcgttggtt gttaaagtat
     8521 tatccaggat ttctttaaac tggcttagct gagcatccat taacattgca ctattaagtt
     8581 tttccagaga ggtttttatc cttttatcat accgttctat ctcagtaata tttttcttaa
     8641 gattagtgtt ggctacccag aagaagagga tggttaccag aatcatacca acgatgaaga
     8701 gtagatattt ttctttcata gttgcacctt ctgtggttgt aatggagcag cttcccgact
     8761 ataacattca atgatgaatt ccagaatttc ggtatcctta gaaatttgtc gcatttgtgg
     8821 tcctgcctta atttcgggga aatcattact tatttccgga ttattcttta atcgttgaat
     8881 aaaattgaat ataacatcac cttcttgaat atttttacta atttcaatta taccattaag
     8941 agtaagaact ccattactat aggtaaattt tctaatggct aattgtttat caatttcacg
     9001 acttaaagct tccaatttct ttgtccagaa aatacggtta ttaaaggttt ttgctaactt
     9061 ttctaaatcc tttgcagata gatattcacc acttgactct actgcagcaa gctgagaact
     9121 gatttgattg tagaatttgc ggcgattatc aactttcttt ttcatatctt tattaatata
     9181 gaaaagtcca acaaacaaaa gagccgcaca aatacagtaa ataataacag cattgcggaa
     9241 agttttattt tctttctcta cttgtagttt taattcccca tatttgttta gatttatttt
     9301 aaaataaaag atattttcca taattacatc ctattctaag cgcatagcca agcctatagc
     9361 taaggcaagt tgcggatctt ttttgtcttc gaatttttct ggcatctcca cattaataaa
     9421 tgggtggaaa acatcagtag ggatattaac tttttcttga atatattcag gtaatcccct
     9481 taatttagca gtgccaccca taaggtagac cttacgaaaa tcgctattac ctgcttcttt
     9541 gacatagaag cgtagggaac gcctcaactc ttcaacgatg gcgtcttcag ttgctttttc
     9601 agaaatgtcc agcatactaa tcgtctgagt tccagctcta ctggggtcat caagtaagcc
     9661 ccattccatc ttatagtttt ctgcttctgg ccattccatt tggcgtttac gcataatatc
     9721 acgagtgaaa ttatagcctc cataaggaat gtcccgggca aagaacttgg cttcaggtcc
     9781 ccatataacc atattggtac gatgggcacc taaatttaat agaatataaa ctccctcttc
     9841 tacaaatgca tttaagataa aactatttgc cacagctaaa gaatctatat caacaatatt
     9901 gggagtcaac ccggcagtag ttagaatatt agtatgttca tttagtaatt cttttgaagt
     9961 ggcagctaac aggatattca tattgttagt tttttcttct acgctaataa cttgataatc
    10021 cagtaccata tcggttccac tgatagggat atgttttttt gcctcaaaga ataaagctga
    10081 ttccaactca tcatcaggga ggaaaatggt ctttatttgt ttaatgctgg tattatctcc
    10141 tcccacagaa gtgaccagat gttgaatttt tttagggttg atatgcagcg tttttagtat
    10201 ttctactatt acaggagcaa aacggtcggg acgcaggtca ctgggaacat attctacatc
    10261 aattggtata gtgggtctga tttcgtagtt taatagtttg aagccttcat gcagtctttt
    10321 taaatgcact accttcacac tgtgactacc aatatcaata cccacagatt ctttaaactt
    10381 gattgctttt gtttttttca ttgttcctct atatttttca ttatatattt aaatgtattc
    10441 tggaggacgt tttataatcc agttaacagc agcaaagggg gtttcgtctt tacgattaac
    10501 ttgagggaaa tctatgggtg aagtatgata aaatctatta tcgtaatgat aatttttttc
    10561 ataacccgta ccagaaccag aagtccccgg atagttaaca ttggtcagtt gaaaaccgaa
    10621 tacagggtca ttatgttgca ctgctgtagg tgcacttgtg ggtccacaga aatcaatagg
    10681 ctgattccat tcatcattgg aaggccattc ttcatcataa tagctgcgat ggacgaatcc
    10741 tcttcttctt tgagaaactg agccgtagat attaatatat cctcgttcca gataaggtgt
    10801 cctctctggc cacagaggat tataccaggg ataatcaatt ttcagattgc ggggaccatt
    10861 acaagttgcc catttatatg cattactctg tggaaaatgg taacgatgaa ggtctatttt
    10921 cgtatataga acattattat atctataatc aggaactgaa gggtgaggat gttgatattc
    10981 aaaggtaaat actccatcct gatgggtatt tccatgtcca tctcctaaag ctgccattgc
    11041 agcataaatc cagatcccac cataacctgt gtctggtccg caggtatcgt ggtgacgtaa
    11101 agtatcaact gggcttctat aaccgtactt aataacgata gatttctcag aaactaagcc
    11161 gacaacatca gtagtattat tagcagggtc cgaacctatg ggagtatttt caagcaaaat
    11221 atcatctacc aggaacattg tatcagcaca tccccaggtc tgataggtgg caaaagttcc
    11281 gcgaatccag agacgattat tcacccaatt agatctatta ttagaagtac catgtgcacc
    11341 aaaagtccaa acagtatctc tcacttgata agtatttgta aataaaggtt cagtagcagt
    11401 aggtggtgga taaggattat aaacatcagc gctttctaac cgaggagatt gtatgtcccc
    11461 tttataataa gtaaaagatg caccatctac ttctacataa actatcacat cgggattata
    11521 attaagaggt ccaacttttt gcccattacg tctaataagt gtagcatttt ccggaaaaac
    11581 aacataatca tatccttctg aaagacctcc agggaaaacc tgactttcag aataacttcc
    11641 tgagtgggat tggatttcac ctgcggtgga gacccaacca taaaatattg gccaaccacc
    11701 attaacaccc ccgtaagttt gcttaatcca gatatcagta ttgctatgaa cacgaccata
    11761 taacatatca ggaccgtgaa aatagacatt agttccatta ggcgattcat cagtatcagt
    11821 aaaatacata aatttagcaa aagtctcagt ttctaatgta taaattccat atttacgaat
    11881 catagaaaat ttagaattca tagctgcaac ttgtccgata ccagttttag atttaaccaa
    11941 tgattcaact atggtttgct gtttctgatc acccataaca acttcttgag aagtaagtaa
    12001 acgtccctta ctaagtagcg actgaatttt aaaagttttt ttcagtgcgg aattggttaa
    12061 ttcaatactt cgctcagaag tacgcaccgg aattaatata gttcccagtt tttgggctat
    12121 tttttgtcct ctataggctt cactacgtag gaaaagagta ctttgaaaat tctcaaaatc
    12181 atattgaaaa gccactacat cgttcatagc catactggat agactaaaac cggacattac
    12241 tgctatcaca cttaataata aggcaatagc aatatttcca ctgggatgtt tgaatatatt
    12301 aaacataaaa cctcctcaaa agaggattac tttttttatt tttttgccat cttagttttg
    12361 aacttaacca tttcatatct atccgcacct gttttaattc ttgcgttcaa ttctacttgg
    12421 actacataga cattcggttc tggattaacc ttagtaaaaa gaaacttatc aattatgata
    12481 tcgtcaagat cttcttcttt cggaaagata tatataggag aagcaacttg aacaccgtga
    12541 tgtacataag tgcaacgcac agcgccatta tataaataaa aatgagcatt atcaaattta
    12601 tatatatctg aagctgatgg aggtattacc tttaatccgg tagaaacatt cgtggtagta
    12661 ttagttagtt ccaaggtcat cgcattaatc acaccaatat attcagtatc ggttcccgtt
    12721 cctactggaa caccattttt aataagattt aagcaattta aagcatcttg ttgtaattta
    12781 gtccaggcag ttaattcttt ataagttctg aagaaagttc caatacctaa cccagaaatt
    12841 aataacaata ataccgatat cgccattacc acaattaatt ctattaaggt tactccattt
    12901 tgccgtgcta atatagatga tttatgtttt ttcatattat ctaaataaag taatcttctc
    12961 gcattctaat aaacctttct ttcttcgtgt cggggtctat ccagcgaagt gtggcttcca
    13021 gatagatata ggtcaataac tgttcattag agctttcttg tgcagttttt tgttctatgg
    13081 tcatatatcc aactaaaata tgatttggat ctctatcatc aatgatgtat tccctattaa
    13141 cttgcaattc aaaaggttta tcatgaacat gccgataata ttccatctcc agttcaccag
    13201 taactaaaag tgttgctact ctatctctat aattaatcag taattgtttt tctgcataaa
    13261 ctatgcctat atagatagcc aggaccataa cgacaataat gaccattacg gctaagactt
    13321 ccaataaggt catgcccttt tgttcctgta atagttttgt tgcagaaaaa gaatgagcag
    13381 aatttctgct attcatcaca caaatcctgc tcctgtagca aattgtttag gattgtcggc
    13441 aaattgttct gctactgttc tttctattaa attattcatt accagattat atagagactg
    13501 atctcgagtt tgcattcctt cttttgtgct agcctggatg actgagggaa tctgataagt
    13561 cttctcttca cggattagat ttcggacagc agtattggca atcataattt caactgctgg
    13621 aacacgaccg gttccatctt ttgtgggcaa gagagtctga gatataactg cttccagaga
    13681 ttctgaaagc atagacctta cttgttgctg ttgttccttg gggaacatat ctataatacg
    13741 gtctatggac ttagtacagc ttcctgtatg cagagttgcg aaaaccagat gtccagtttc
    13801 tgctgctgtc aatgctaatg acaccgtctc taaatcacgc atttcaccaa ccagtatcac
    13861 atcaggatct tcacgcaaag cacttcttaa agcagcagta aaactccagg tatcatgccc
    13921 taattctctt tgatttatca gactgttttt actctgatga acgaattcaa tagggtcttc
    13981 aatggtgata atatgacaat aacgagattc attaatagaa tctatcatcg tagccagagt
    14041 ggttgatttt ccacttccgg ttggacctgt cactaaaacg aatccttttt ccttctgagt
    14101 aagtcttttc aggatatccg gtaaatgaag ctcctcataa cttttaatct cattaggaat
    14161 gactctgaag gcgcaagaga tgccattaat ttgatgaaaa gcatttaccc ggaaacgaac
    14221 atcgttactt agtttggtag agaagtcaat ttccagttgt tctttgaaca tatcttgttg
    14281 aacttcattc atcacaccaa aaaccagtga ttctacttct tctggggata ggatgggtag
    14341 attcagacgt ttcatcttac cattcacacg aaccataggg tgcgctcctg cagctatatg
    14401 caggtcagac gcacccgctt ccgctgtgaa ttgcaataat tcatagatag tcaacttact
    14461 gcctccactt attatttccc tgtttcggtt tccattgcct ccgtcatagg gttcgtaaac
    14521 tcatctatac cgtaaccatg gaactttgct tcttgaacat cataccagac ctgtttatca
    14581 ggaccgccag gaaattcttc agtggaagta gcaatgaatt tcttcggtgg attgccaaca
    14641 acttcaaatt tccagttctt taaagtgctt tcacctaaat tggcatcttt catagccttt
    14701 tcaatggtgt agtcctgggt ggttcctgtg gtttgacggt atacatcata ggttttacga
    14761 atggcagcta ttgccgtctg agcctcagtg gaacgagact tttccacata tctcaagtat
    14821 ctgggaaccg ctaaagcggc aaggatggcg acaataatga ccaccactaa tacttcaatc
    14881 agggtaaatc ccttttgtcc ttgaagttta ttaagcattg taatttcctc cttatcggat
    14941 ttttcttata tttttttata tttgcacaat gcgtgccaaa tctcaaattt tatttaaacc
    15001 cacaatagaa tcttataaat atcttgactc ttttttgtct atactttttt tgacttttca
    15061 tatattaata ctcaattgag gagaaaataa tgcattttaa aaaatggttt attgtaaccc
    15121 ttgtatctct catagctatt actacccttt gtgctgataa tttagcccct gtagaggttt
    15181 ctaaacagaa cagaataaat gaaactgata ttttagcaga atttgatgga ggagttatta
    15241 cacgggctga cctggaaaaa agaatttcaa tgcttccacc taatgtccag ggaagataca
    15301 aaacaatgga aggacaaaaa caggttttag atgcgattgc tatggaagaa gtttttgctg
    15361 ccaaagctat gcaactaaag ctgaatgaag accccgaagt cctcgctaat attgaagcaa
    15421 caaaaaaaca gtatcttatt caggaatttt acaaaagaaa tataactgac cgggtagcgg
    15481 tcactgaaga agataaacaa cgctactatg aagaaaataa aaaattgttc tatgtaaatc
    15541 ctaatatcac tgccaatatt attcaattac cagatgaaga aacagctctt caagcaattc
    15601 aggaactccg ttctgggaaa agttttgaag aggtttccaa tctttataat ataaataatt
    15661 atataaagag tttgaagggt gtggtaaaaa atatccggct aaatggaaat attcctggaa
    15721 tcggtaatga ttatgaactg gaagacctta ttgctaaagc acaacccgat acaacagcca
    15781 ttcttggtcc ttataaaact acaactggat ggtatgtttt ccgcgttata gattatcaac
    15841 ctggttatca aaaaacctat gaggatgttt attctgaact ggaacagcgt gctaaaccag
    15901 aagtggagaa ccgtttactc aatgctctta tgagtgattt aaagaccaaa tatgcagtgg
    15961 ttgttgattc cactctcgtt aatcagattg atttgagaaa tccctctaaa aataaagata
    16021 ttgaagaaaa agtcctggtc actgcttctg agccggaatt gactattaca gtaaaatcct
    16081 tgctggatag tttttcaaaa ctttctcctc aagaacaact ctttttcact aaaggcggtg
    16141 gagcaaatca atttctggaa caagaactta ttcgcaacct gctttatctg gaggctaaaa
    16201 atcagaatta tgaagagtat ctaaaagata atgaaaatta tcagcagatg caacgctatt
    16261 atatgctcca aaaagcatat aaaatacttg tagttgattc cattaaaatt gacagtgaag
    16321 atactcgtgc ctattatgat gcgcatatca atgattttac tactccaggt tatagaacta
    16381 tcaaggttct ctggtttgat gatgaaaaaa ctgctgagtc cgttcgtaaa aaatatgctc
    16441 tttacactgc ttttgatgat actacaagga ttaataaatt aattgaaaaa gaatccaaaa
    16501 aacctaactt atcagtttta gaccatatat ataataatgg tataattact ggaataggac
    16561 ccgataaaga cttttgtgat atggtctgga ataatcccat tggatatatt agtcctgtat
    16621 tcaaaactgc ccgtggtgat attgtctttt tccagattat tgcagaaacc cctgctcagg
    16681 ttaaatcttt tacagaagtt gaaccaagta tctataatat tcttagaaga gatagacaga
    16741 tggctcaaca ggaaaaagtg acccgggaac tgtacgaaga gtttcatatg gttctttatc
    16801 cagag
//
LOCUS       DAORZF010000037        16662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_2816_length_16662_cov_355.762329, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000037 DAORZF010000000
VERSION     DAORZF010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 16662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16662
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2816_length_16662_cov_355.762329"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2387)
                     /locus_tag="PK015_05345"
     CDS             complement(<1..2387)
                     /locus_tag="PK015_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33926.1"
                     /translation="MKHIALILIIGCVGFCLWGAKWETITNTTHIYDLLEMDTGYYMG
                     TWGGMVFFPKLNTPGIHLEAINKVWTTADGLVSNDVRNISYIQFNQSIWLGSASDGIT
                     IINQQGMQKLTTELGLPSNNVVKIVEKGSNVLVATQMGLANYYYLEGVNFPLQLHQYN
                     VQNTPGLLSNNINAMELASNNYLFISTDRGLNFVHLDSLDVDDAWHSLSGARVKNGHK
                     NKLSIGGDKLIVTAPTEIYCHSIDPYQNGWTHYTSANGLINQNFNYATIDANGDLWVS
                     YGTWDEDILAYNLTGDILLTRISSGGAIQHLNKKELGLGTKCISKIICTDDGIYLCSW
                     GDGIFYQIYPFNPAADPWQQYLQNTIGFPKIRNIVTDAQNAIWFSSGAWSIIPQRKSS
                     LGACRYKDGIWRTYNLANSPIHTDNVYTVEVDSKDRKWFGTFYAIEDASPTGWKYGVS
                     IFDEVNNQWFWIMHSGMYQWNETTQSYGGNPIGTAQVMGDTDVGINCDKYGNMFIACC
                     EDGFTVLSPEDEFIRNFTIPNSVRQAVEYSYHSGRQYFIGTHNDRGLVIWNDDSIPES
                     NGNHWLIPDPPELNNCEVYGVVTIESPYEGTQHWIAASNGLFMWDETDWYKYDTAIKR
                     YKYNKNTHNWENNILYFVDEERLFGSVRTTPTAIFLDPFNRIWIGSLQHGLTMYDPIT
                     ERFTNYYQENSPLLSNYITALGYNPEEGKLLIGTPEGLNTLIIGYQIKPETQLTSLKI
                     YPNPFYPEKDKSISIINYPSGTLPVGNNKCRIYDSSGALVITLKENEFARFDWDG"
     gene            complement(2384..3433)
                     /locus_tag="PK015_05350"
     CDS             complement(2384..3433)
                     /locus_tag="PK015_05350"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOL33927.1"
                     /translation="MNYIIIVIIILAIAIHIATHLLVPLNIKLSRKLHIVAQPNERKI
                     NQIAIPEAGGLSFALPIIIAELILASIIPDVEFKLLVFPLIGVELITLILGITDDRWD
                     IPALLKLLWQIGIGIIMYLIEFRVMSLTNPFGSDLMLGWLSFPVTIIWYLVLMNAINL
                     IDGIDGLASGICVIVCAVLLVVGIKESNSIVIALSAFLLAGNLAFLRFNFYPAKIFLG
                     DTGALFNGLVLAAISTAGNRQYKGITSMTLIIPLSVLAVPLIDMFLAIFRRIRGGNNI
                     FTPDKKHLHHIMLSLGLSQRMVAIIVYFMTLLFGLIAIGFSFSTKKILFSVLLCLLTL
                     AVVVSYIFMRKGRKE"
     gene            complement(3430..4551)
                     /locus_tag="PK015_05355"
     CDS             complement(3430..4551)
                     /locus_tag="PK015_05355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOL33928.1"
                     /translation="MTRLLHLQLLPLLSGVQNFSLFLLDGLPENQFEIYVASVPGGDL
                     VDAVKARGYHYLPLKHMKRPIAIDDTLALMELYSLMKRYNFDIVHTNSSKPGFLGRLA
                     ASFSHIPLILYTAHGTSFHDDQPLWQQKIYRQLEKWANSWGDYVVFVNSCDRLKYLEM
                     GLVPPSKAITIYNAIPYEQVEKFTKIAQKRTCNPSKREFVIGSTLRFSHQKNVINLIT
                     AACQACKNEPKLKFIILGEGEHWNICRTIVHSYGLEERIILPGWDVNIAQWLALFDAF
                     ILYSRWEAQPFSVIEAMHSGLPVIVSDIPSLREMITEECGWIVPLDNQPALIQCLIEV
                     AQHPEIAFEKGVIANKRIKEISDYDTMVKSYLELYQKVM"
     gene            complement(4548..6248)
                     /locus_tag="PK015_05360"
     CDS             complement(4548..6248)
                     /locus_tag="PK015_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33929.1"
                     /translation="MIKSNIYGIPSMIYIFIDYKLEKFKNEINYTYGFIFQTLGYSFS
                     FVSDIGELKEDDILFIYGYTDPTIEELVAIAQHFITIFIPCEPDLFDPKSMTMDKLKR
                     NLKMVKLLSKTPVISSRSFSYPAENYSESKIQAGKINFDLVGNIFFHLAHLESQVDST
                     RDAEGYYPEEASIFHPYRETPYVDNMLWLVDSMIKEHTRSKGIPLVQKQYWPQGQEGA
                     ALLTHSVDNLQKWNYTDLLLSLGTDVAMFFTLNWKQLFHTISSKFKYLFTNVELYWNF
                     EEYRALEEASGYRSTFYIAPDKNEYINYSLDDPDLQEEIRQLTRTGNEIGLLLTPDKT
                     NPDDYITRKQVMLHQLHQETIGIRQLHYKINENLRGLHSKLNPAYSQSTALRDMPGYI
                     NGVTVPYQPWIGGLSVPWYELPTIYCDAHLRVGRFKTLQLEPAKKLLKKFFQSTIRTR
                     GVFGLDLNLASYYDIPYVKKLYTYALALIKSGGIWVPTANELSQWWNKRNRVTINETE
                     YEISIFFPDNLENFTLKIINIKNIKEILGVPAKVEGNMVSFSNVNADSIAVIRLNQEL
                     "
     gene            6370..6846
                     /locus_tag="PK015_05365"
     CDS             6370..6846
                     /locus_tag="PK015_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HOL33930.1"
                     /translation="MEKRPSWQQYFMQMAFLASTRSTCLRRQVGAVLVKDNQILSTGY
                     NGSPKGVRHCAEVGCLRKKNNVPSGQMHELCRGLHAEQNAIIQAGLNGSSARGATLYC
                     THQPCSICAKMIINAEIQTVYVAEKYPDELAEKLLAEAGVELILYNPQTGKMKKLI"
     gene            6846..7388
                     /locus_tag="PK015_05370"
     CDS             6846..7388
                     /locus_tag="PK015_05370"
                     /inference="COORDINATES: protein motif:HMM:NF021348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOL33931.1"
                     /translation="MSKVFRTFTTRDLIIIAGLAGIGLAIKPLVSPLSKMITSPLMIP
                     GGSFTGGLYMLWMVLAVLIVDKFGTGIVYGLLQALVIMVIGIRGNQGLLSLVSYTFPG
                     LLVDLVYLAIPHPERIWGQMLLCALANMAGALIVAIFVFHHPLPFILIIESMSMISGI
                     IGGWISWAIYKTIKHYELVQ"
     gene            7385..8308
                     /locus_tag="PK015_05375"
     CDS             7385..8308
                     /locus_tag="PK015_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33932.1"
                     /translation="MKKFFLFLPFVTISILSAIIITGPQGDSLIYSYTELAKIPRETF
                     TTTRVKSGEIQEDIWTGFRFNHWFNDNIKIPYKIIRFESADNYMVSFSKAEFDSLECW
                     LAFTQNGQVLPENGIRLIFPQLRDMKWIRGLNRVVLEDFSPLKLPARFEFLDKRLKQE
                     TLIENPPPFSDTKGYYFADLLPLSARNDTHSVVLYSSDGIKCSLEYPRHLKDAIIELT
                     DYGFNLKSPHIPDGMWLKDIIYLQIDDWALIKSDNLDALITLNRIMDWKLSPDVQFIV
                     NIGGKEEKIPLSEVLAYPDKLANITSFELIP"
     gene            8387..9034
                     /locus_tag="PK015_05380"
     CDS             8387..9034
                     /locus_tag="PK015_05380"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOL33933.1"
                     /translation="MLKVQNLYLCYPGQSEPIFQNLTFELKDGELLWLTGPNGCGKTS
                     LLNCISGIIPQRLKAEFKGDIILNDLDLNPIPINERFRYLAYQMSDPDNQIFFPRVEK
                     ELSFALENAGLTIKEMKERIIRATEDFGLQDFWETEPGKLSLGQKKLLVLAFCSAQDT
                     PLYLLDEPTSALSGESILKVEKWLKNLLSNGKIVIMVEHNPDLGSLATQILDLGK"
     gene            9031..9675
                     /locus_tag="PK015_05385"
     CDS             9031..9675
                     /locus_tag="PK015_05385"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOL33934.1"
                     /translation="MNSTLFQINNITFAYPKNPPLFQDFSFEIKQDDKILLYGANGSG
                     KSTLLALLMGMLTPQQGNITLNDTSVQELTPDRYNEVLYSRQNARMDLFGLVPRNDWE
                     LWHLALPDIFTEQSLLLADSDLIAKYDTPYARLSGGELRAFSLLWLPLLQDRFWLLDE
                     PTAGLDAKHKKDFVERCLNKNERGYLIVSHDPVLTENLFTRIILLEKGRLTKIK"
     gene            9672..10400
                     /locus_tag="PK015_05390"
     CDS             9672..10400
                     /locus_tag="PK015_05390"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOL33935.1"
                     /translation="MNKLHPLSFIIAALWFSTAAIFVPQLPWLTLAFLLCAIFRGLLT
                     GFNFRLWWKQVRKMIPLFLAILVIQLLFTKKGELLWGKSWYAIYSVGLHRGIAVSLRL
                     LILFFSAQILLRLNYEDFDIAFNTLHLPEEFAFMIFYTIHIIPSAGEHIQHSLQLVKL
                     RGINMRKLPLKSQLTIYRYISLSVLADVLSRSEIQAIALDLRGFRSTGKHTHLLQKKF
                     SLYDLMLYLSITIITLVYLFSILA"
     gene            complement(10405..>13200)
                     /locus_tag="PK015_05395"
     CDS             complement(10405..>13200)
                     /locus_tag="PK015_05395"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HOL33936.1"
                     /translation="YSVNHIWSESDKADSLTGFDVQMYELTLSINEQTHYINGNVLAT
                     VLAEENLSYIEYNLVGLTVNNVLVNNIPTTYTHSNGIIHINLNISAGSTFTTQVFYSG
                     IPQLSSDIYHIGMIFTANSVFTISDPDAARYWWPCYDHPWDKAIINLTITIRSDWKVA
                     ANGLRTNIVDNGDGTSTTYWIGQYPMTTYLVCVTCGPYVEFDQSMEELPIINFVLPGQ
                     YNNALITLSRVPMMIDYFSQLFGPYPFEKYGHAVVNMSTYGAMEHQTMTTLGNYIING
                     TLAYETTIAHELAHQWYGDAVSFLTFKDVWLSEGFATYSEHLWKDKTEGWESACNYIR
                     TEFHNYYINWENTEGPHTIYNPNFNSYFAPPSYEKAASVLHMLRLKIGDANFFSLLQN
                     WYNTYCYGNAITSEFQAMAEQISGQDLDQFFQQWIYSPGIPSVEYSVWFNNESNSPLK
                     IVAKTISNTSTPFYLEVPFRLTQAGISDSLFVQASPEGFNNYFNITLGAGAVFNPNYH
                     NWTLLRSITEKRPVLVECLPTNNSVFLSWESFTDSPDLGYRIYRRNVDNGSEWIALNS
                     EPLYSLSFIDNTAINGTLYQYAISAIDSEGWSSLQSNIMSAIPVLFSFAQDFLVVDET
                     RDGNGSNISPDDAMVDDFYNAALTPIEYDSWDVASEGLPDLATLGGYKVVLWHADDLA
                     ENLLVEHQSILGSYILGGGKVLISGWKTAIVLTETFLQRFAGIPQIVYDNVPCLISAQ
                     STIYPELWVDSNKLIPAWNGMLPYICTFPEAEQIIYIANMNPAIPHNGNCLALRHSCN
                     NGELILLGFPLYYMQAEGVRDFLQLIIPELINTPVIDSTIPSVVASMKIYPNPFHKDC
                     HIQISLADKGLASLSLYNLKGQKVKTFFQEAKMKGDYTFNFDGKDDNGKVLPSGIYIL
                     RLKQSDRIITRRISYIK"
     assembly_gap    13201..13300
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13301..14598)
                     /locus_tag="PK015_05400"
     CDS             complement(<13301..14598)
                     /locus_tag="PK015_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOL33937.1"
                     /translation="MNSDQTDFLEEKEITDRNVPLAEKLRPRSLDEVLGQPALTAPDS
                     PLRKMIESDEYQSFILWGPPGSGKTTIARIIENSTKHRFVRFSAVLSSITDVKAVMKD
                     ADYMHRHQNQTTIVFIDEIHRFNKAQQDAFLPYVESGAIVLIGATTENPSFEVIPALL
                     SRCHTFVLQMLSEEDLKTIIRKGYESLGLEEDEDIIGWLAQQSSGDARRALNQLELLA
                     PYLKKEPHKEIKQLATILEKKTLYYDKDREEHYNLISALHKSLRGSDPQAGLYWLARM
                     LEAGEDPRYIVRRLIRFASEDVGLADPQALVQAIAVKEAVLFIGMPEASTALAQLVVY
                     LATAPKSNSLYTAYEAAAEDARKTSHYGVPFHIRNAPTKLMKDLGYGAGYKYDHDFDN
                     KYAYQKYFPEQMEEKTYYIPGEFGFEKEIAKRIQWWIKLR"
     gene            complement(14602..15069)
                     /locus_tag="PK015_05405"
     CDS             complement(14602..15069)
                     /locus_tag="PK015_05405"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOL33938.1"
                     /translation="MDRKELYNFAIAQEQRSQRLYQGLARSFGEPETNAFFTELVGLE
                     KEHELKLRSAFSQEFPGEEPEPETQEIKEIPSVDLKDPAQLLQFAITREEEAAKNYYS
                     FAEQTEPVDIKELLLHLAKEEEQHKTLLLTEMQRILGALEWFDPSELDGFMDF"
     gene            complement(15083..16342)
                     /locus_tag="PK015_05410"
     CDS             complement(15083..16342)
                     /locus_tag="PK015_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOL33939.1"
                     /translation="MKQKNRSIIWLAKRYLRGQSAFFINRSHFLTLIGLTLGVTGLLC
                     VSSVMNGLRTDIQERITGTLSEIKISRPDGAPFSEYDKMVDKLNQEGFLSAPVVRNEL
                     VIIKEGKLSPTLSLGIDPNRHSKISSVFQPKEMDNSAGFQGLLAGNIQSNEFTDGGIA
                     MGIGLAASLDVRIGDEIELLSPVFNVPSAFGMLPRVRKLKVTAIFTTGMPEYDETYSF
                     IPLSIAQFFSGYTNEIDYIEVKTGAFSNPQRATRKIKALFPEFEVEDWSAYDPSLYSS
                     IRFEKNLMFLIMLFMYIIASFNLIGNLWKTITRKKKELGLLKAFGYKESELGTLFLYQ
                     ALFLATLGIALGLIIATVLLLIQQQYGIISMDLGTAGLSALPVKFATSDYLMVIIFSY
                     VVTFLSVILPLRKLKNINPVELIRQTA"
BASE COUNT         5155 a         3296 c         2864 g         5247 t
ORIGIN      
        1 aagccatccc agtcaaaacg agcaaattca ttttctttta aagtaatgac caaagctcca
       61 gaactatcat agatgcgaca tttattattt cccacaggta aagttcctga aggataattg
      121 atgatgctga tagatttatc cttctctgga tagaaaggat tgggatatat ctttaaagat
      181 gtgagttgag tttccggttt tatctgataa cctataatga gagtattaag accctctggt
      241 gtgccaataa gcagcttccc ctcttctgga ttgtatccca gagcagttat ataattagat
      301 agcagaggag aattctcttg ataataattg gtaaatcgtt ccgttattgg gtcatacatt
      361 gttaaaccat gttgcaggct accaatccag atacgattaa aagggtctaa aaaaatcgct
      421 gtaggagttg tacgaacaga accaaaaagt ctttcttcat ccacaaagta gaggatatta
      481 ttttcccagt tatgcgtatt cttattatat ttatagcgtt ttatagctgt gtcatattta
      541 taccaatcag tctcatccca cataaaaagt ccattactgg cagcaatcca atgctgagtt
      601 ccttcgtaag gactttctat agtaactact ccatacactt cacagttatt caattctgga
      661 gggtcaggaa taagccaatg gtttccatta gattcaggga tagaatcatc attccagatt
      721 actaaaccgc gatcattatg tgtaccaata aaatattgtc taccactatg atagctatat
      781 tctactgcct gacgaacact atttggaata gtgaaattcc taataaattc atcttcaggg
      841 cttaggacag tgaaaccatc ttcacaacag gcaataaaca tattcccata tttatcacag
      901 ttaataccca catctgtatc tcccataact tgtgccgttc caatcggatt tccaccataa
      961 gactgagtgg tttcattcca ttgatacatc ccactatgca taatccagaa ccattgatta
     1021 tttacttcat caaagatgga gacaccatat ttccagccag taggactggc atcttcgata
     1081 gcataaaaag tgccgaacca ctttctatcc ttactatcta cttcaacagt atagacatta
     1141 tccgtatgaa tagggctatt tgccaagtta taggttcgcc agattccatc tttatatcta
     1201 catgctccca gagagctttt cctttggggt attatactcc aggctccact ggaaaaccat
     1261 atagcatttt gagcatcagt gacaatattt ctaattttag gaaagccaat ggtattctgt
     1321 aaatattgtt gccaagggtc agccgctgga ttaaaaggat agatttgata aaagattcca
     1381 tcaccccagc tgcaaagata aatcccatca tcagtgcaga taatcttgct gatacatttt
     1441 gtgcccagtc cgagctcttt tttatttagg tgttgaattg cacctcctga actgattctg
     1501 gtaagaagaa tatctcctgt cagattgtaa gccagaatat cctcatccca ggttccatac
     1561 gaaacccata aatctccatt tgcatcaata gtcgcataat taaagttctg atttattaat
     1621 ccatttgccg aagtatagtg agtccaacca ttctggtaag gatctataga atgacaatat
     1681 atttccgtgg gagccgtaac tattaattta tctcctccga tagaaagttt attcttatgg
     1741 ccattcttta ctctcgcacc cgataaacta tgccaggcat catccacatc cagagaatcc
     1801 agatgaacaa aatttagccc tctatcagtg gaaataaaga gataattatt actggctaat
     1861 tccatcgcat taatgttatt agatagtaag cctggagtgt tttgcacatt atattgatga
     1921 agttgcaaag ggaaatttac cccttccaga tagtagtaat ttgccagtcc catttgagtt
     1981 gccaccagca cattagaccc cttctcaact attttaacta cattgttaga aggaagtccc
     2041 agctcagtgg tcagtttttg cataccctgt tgattaataa tagtaatacc atcactggca
     2101 gaacctaacc atatactttg attgaattga atataggata tatttcgcac atcgtttgag
     2161 accagtccat ctgctgtcgt ccagactttg ttgatagctt ctaaatggat acctggtgta
     2221 ttaagcttag gaaaaaaaac catcccaccc caggtgccca tatagtatcc tgtatccatt
     2281 tctaataaat cataaatatg agtggtatta gtaatagtct cccatttagc accccagagg
     2341 caaaaaccta cacaacctat gattaatata agagctatat gtttcattct ttccttccct
     2401 tgcgcataaa aatatatgat accaccactg ccagtgtaag taaacataat aacacagaaa
     2461 atagtatctt tttggttgaa aatgaaaaac ctatggctat caagccaaat agtaaagtca
     2521 taaaatagac aataatcgct accattcttt gagaaagtcc cagagaaagc attatatgat
     2581 gtaaatgttt cttatctggt gtaaagatat tgttgccacc tctaattctg cgaaatatag
     2641 ccagaaacat atctattaga ggcactgcca gcacggatag tggaataatc aaggtcatag
     2701 aagttatccc tttatactgt ctgttccctg cagtagatat agctgcaaga accaatccat
     2761 taaataaggc tccggtatca cccagaaaaa tcttggcagg atagaaatta aatcgcaaaa
     2821 aagcaagatt accagccagt aaaaaggctg ataaggctat tactatgcta ttgctttctt
     2881 ttattcctac tactaataac actgcacata caataacgca gataccactt gccagaccgt
     2941 caataccatc tattaaattg attgcattca tcaagacgag ataccagata atagttacgg
     3001 gaaaagaaag ccagcccagc atcaaatctg acccaaaagg attggttaag gacataactc
     3061 tgaattctat aagatacata ataatgccaa ttcctatctg ccagagcagc ttaagcagag
     3121 caggaatatc ccatcggtca tctgttatac ctaaaattaa agttataagt tctaccccta
     3181 ttagcggaaa aaccaaaagc ttaaattcta catctggaat tatacttgcc aggattaatt
     3241 cagctataat tataggtaaa gcaaaggata aacctcccgc ttcaggaata gctatctgat
     3301 ttatcttccg ttcattcggt tgagctacta tatgtaattt cctggataac tttatattta
     3361 agggcaccag aagatgagta gctatatgaa tggctattgc aagtataatt ataactatta
     3421 taatataatt cacatcacct tctgataaag ttctaaataa cttttgacca tcgtatcata
     3481 atcagatatc tcttttatcc tcttattggc aataacgcct ttctcaaaag caatctctgg
     3541 atgttgtgcc acctctataa ggcactgaat caaagcaggt tgattatcca gtggaactat
     3601 ccatccacat tcctctgtaa tcatttctcg taaagaagga atatcactaa ctatcactgg
     3661 taaaccggaa tgcattgcct ctatgacact aaaaggctgc gcttcccacc ttgaatatag
     3721 tataaaggca tcaaataaag ctaaccattg cgctatattt acatcccaac ctggaagaat
     3781 aattctttcc tctaaaccat aagaatgtac tatcgttcgg caaatattcc agtgttcccc
     3841 ttcacctaag ataataaact tcagtttggg ttcattttta caagcctgac aggcagcagt
     3901 aatcagattt ataacattct tttgatgaga aaaacgaagg gtagaaccaa taacaaattc
     3961 tcttttagac ggattacaag ttcgtttttg agctatcttt gtaaactttt ccacctgctc
     4021 ataaggtatg gcattataga ttgttattgc tttagaagga ggcactaacc ccatttccag
     4081 atatttgagt ctatcacaac tattcacaaa taccacataa tcaccccagc tatttgccca
     4141 tttctccagc tgtctataga ttttttgttg ccaaagaggt tgatcatcgt gaaaggaagt
     4201 tccatgtgct gtatacaaaa tcaaaggaat atgagaaaaa gaagctgcta atctacccag
     4261 aaatccaggt ttggaagaat tagtatgtac aatatcaaaa ttatagcgct tcatcaacga
     4321 ataaagttcc attaaagcta aagtgtcatc tatcgcaata ggacgcttca tatgtttcaa
     4381 aggaaggtag tgatagcccc gagctttaac agcatctacc aaatctcctc ctggaacact
     4441 ggcaacatag atttcaaatt gattttcagg taaaccatca agcagaaaga gactgaaatt
     4501 ctgcacacct gagagtagtg gtaaaagctg aagatgcagc aatctggtca taattcttga
     4561 tttaacctga ttacggcaat tgaatcagca ttaacattgg aaaagcttac catattccct
     4621 tctactttag ctggtacacc cagaatttct ttaatgtttt taatgtttat tatcttcaag
     4681 gtaaagtttt ccagattgtc cgggaaaaaa atgctgattt catattccgt ctcattaata
     4741 gttactctat ttcttttatt ccaccattgg gataattcat ttgccgtagg cacccagatt
     4801 ccaccggatt taataagtgc caaagcataa gtatagagct ttttaacata gggtatatca
     4861 taataagaag ccagattcag gtcaagccca aaaacccctc ttgtccttat agtgctctgg
     4921 aagaatttct ttaaaagctt cttagctggt tccagttgta aagttttaaa tctaccgacc
     4981 cttaaatgtg catcacaata gatggtcgga agctcatacc agggaacact taatccacca
     5041 atccagggtt gataaggaac ggtaactcca tttatataac cgggcatatc tcttaaggca
     5101 gtgctttgac tatacgctgg attaagcttg gaatgcaatc ccctgagatt ttcatttatt
     5161 ttataatgca actgtcttat gcctatagtc tcttgatgta gctgatgcaa cataacctgt
     5221 tttctggtaa tgtagtcatc aggattggtt ttatccggag tgagtagtaa tccaatttca
     5281 ttgcctgttc tggttaattg tctaatttct tcctgtaaat ccgggtcatc cagactataa
     5341 ttaatgtatt catttttatc tggtgctata taaaaagtac ttcggtaacc tgaagcttct
     5401 tccagtgcac gatattcctc aaaattccag tagagttcca cattagtaaa cagatattta
     5461 aatttactgg agatagtgtg aaatagttgt ttccaattca aagtaaaaaa cattgctaca
     5521 tcagttccca gagataggag aaggtcagta taattccatt tttgtagatt atccaccgaa
     5581 tgagttaaaa gtgctgctcc ttcctgtcct tgaggccagt attgtttttg caccagaggg
     5641 attcctttgc tgcgagtgtg ctctttaatc attgaatcca ctaaccagag catattatct
     5701 acataaggtg tttcccgata aggatgaaag atgcttgctt cttcaggata atagccttcc
     5761 gcatcacggg tggagtcaac ttgtgattcc agatgggcta aatggaaaaa gatattgcct
     5821 accagatcaa agtttatttt ccctgcttga atcttacttt cagaataatt ttctgccgga
     5881 taggaaaagc tacgactgct tattactgga gtcttggaaa gaagtttaac catctttagg
     5941 ttgcgcttta atttatccat tgtcatactt ttcgggtcaa ataagtccgg ctcacaggga
     6001 ataaagatgg taataaagtg ttgtgctatg gctacaagtt cttctatggt tgggtcagta
     6061 tagccataga tgaatagaat atcatcttct ttcagctctc ctatatccga aacaaaacta
     6121 aagctataac ctaaggtctg aaaaataaaa ccataggtgt agttaatctc gtttttgaac
     6181 ttttccagtt tataatctat gaaaatatat atcattgatg gaataccata aatattactt
     6241 ttaatcataa aaaaggaatt ttagcttatg tcaagaaaaa tcatttaatt aaagttgctc
     6301 ccttctttct tgacaaaatt agctgagtta taaagtaaat ctatatttaa ctaatatcag
     6361 gttattgaga tggaaaaaag accctcctgg caacagtatt ttatgcaaat ggctttttta
     6421 gcttctacca gaagcacctg tttgcgaaga caggtcggtg ctgttctggt gaaagataat
     6481 cagattcttt ccactggcta taatggaagt cccaaaggag ttaggcattg tgctgaagta
     6541 ggttgtctac gaaaaaaaaa taatgtgcct tctggacaga tgcatgagct ctgtcgcgga
     6601 ttacatgctg aacaaaatgc tattattcaa gctggattaa atggttccag tgccagaggt
     6661 gccactcttt attgcactca ccagccctgt agtatctgtg ctaagatgat aatcaatgct
     6721 gagattcaga cggtttatgt agctgaaaaa tatcctgatg aattagccga aaaactttta
     6781 gctgaagcag gagtagaatt gattctttac aatccccaaa ccggtaaaat gaaaaaatta
     6841 atctgatgag taaagttttc cgcactttta ccactcgtga cctgataatt attgctggat
     6901 tagctgggat aggtttagct attaaacctc ttgtttcacc tctttctaag atgattactt
     6961 ctcctttaat gattccaggt ggctctttta ctggtggatt atatatgtta tggatggtcc
     7021 tggctgtatt gatagtagat aaatttggca ctggtatagt ttatggactt ctgcaagcac
     7081 tggtaattat ggtcattgga ataagaggaa atcaaggtct tctcagtctg gttagttata
     7141 ctttccccgg gttattagta gatttagtct atctggctat acctcatcca gaaagaatct
     7201 ggggtcaaat gctactttgt gctcttgcca atatggctgg agcattaatt gtagctattt
     7261 tcgtgtttca tcatcctcta cctttcatcc tgattattga aagtatgtct atgatttctg
     7321 gaataatagg tggctggatt tcctgggcta tttataaaac tataaaacat tatgagttgg
     7381 tacaatgaaa aaattctttc tctttttacc tttcgtgacc atatcaattt taagcgctat
     7441 tatcatcacc ggtcctcaag gtgattcttt aatctattct tatactgaat tagcaaaaat
     7501 ccctcgggag acctttacta ccaccagagt taaaagtgga gaaattcagg aagatatctg
     7561 gactggcttt cgttttaatc actggttcaa tgataatatc aagattccct ataaaattat
     7621 tcggtttgaa tcggcagata actatatggt aagttttagc aaagcggaat ttgattcctt
     7681 agaatgctgg ctcgctttca ctcaaaatgg tcaagtctta cctgaaaatg gtattcgttt
     7741 gattttccct cagcttagag atatgaaatg gattcgtggt ctgaatagag tagttctgga
     7801 agatttttct cctttaaaat tgcccgcccg ctttgagttt ttagataaac gccttaaaca
     7861 ggaaacttta attgaaaatc cacctccttt ttccgatact aagggttatt actttgccga
     7921 tttattaccc ctatccgcga gaaatgatac acatagtgtg gtactttata gttctgatgg
     7981 tataaaatgt tctctggaat atccacgcca tttaaaagat gctataattg aattaaccga
     8041 ttatggattc aatctgaaaa gtcctcatat tcctgatggg atgtggctaa aagacatcat
     8101 ctaccttcaa attgatgatt gggcactcat caaaagtgat aatctggatg ctctcatcac
     8161 cttaaaccgc attatggatt ggaaactcag tcctgatgtg cagttcattg taaatatagg
     8221 tggaaaagaa gaaaaaatcc cgctaagtga agttctcgct tatccagata aattagctaa
     8281 tatcacctct tttgaattga taccataatc aataccttcg tttccccttt aatttttatt
     8341 aaagtccaaa acttccatct ttgaacttta tcaaagcctt tttattatgt tgaaagtcca
     8401 gaacctatat ttatgttatc cagggcaatc agaaccaata tttcaaaact taacttttga
     8461 gctaaaggat ggtgaattgc tctggttaac aggtccaaat ggttgtggga agaccagcct
     8521 cttaaattgc attagtggaa taatccctca gcgtttaaaa gctgaattta agggagacat
     8581 catattaaat gaccttgacc tcaatcctat cccaataaat gaaagattcc gctatcttgc
     8641 ctatcaaatg agtgaccctg acaatcaaat cttcttcccc agagtggaaa aagaactttc
     8701 ttttgctctg gaaaatgcag gtttaactat taaagaaatg aaggaaagaa tcatccgagc
     8761 cacagaagat tttggtttgc aggatttctg ggaaacagaa ccaggaaaat tgtcgctggg
     8821 acaaaaaaaa ctcttagtac tggctttttg ttccgctcag gacacacctt tatatctact
     8881 ggatgaaccc acctctgctt taagtggtga atctatactt aaggtcgaaa aatggcttaa
     8941 aaacctgcta tctaatggga aaatagtcat tatggtagag cataatccgg acttaggttc
     9001 actggcaact caaattctgg atttaggaaa atgaattcca ctctctttca aataaacaat
     9061 atcaccttcg cctaccctaa aaatccacca ctattccagg atttctcctt tgaaattaaa
     9121 caggacgata aaattttact ttatggggca aatggtagcg gcaaaagcac tctcttagcc
     9181 cttttaatgg gaatgttaac tcctcaacag ggaaatatca ccttgaatga tacttccgtt
     9241 caggaattga ctccagatag atacaatgaa gttttatatt ccaggcaaaa tgccagaatg
     9301 gacctctttg gtttagttcc tcgtaatgat tgggagctat ggcatctggc tttaccagac
     9361 atatttactg agcaaagtct tcttttggca gactccgatt taattgctaa atatgacact
     9421 ccttatgctc gtctttctgg tggtgaatta agagcttttt cactcttatg gttgccctta
     9481 ttacaggata gattctggct actggatgaa ccaactgctg ggttagatgc aaaacataaa
     9541 aaagattttg tagaacgctg cctgaacaaa aatgagagag gatatctcat tgtaagtcac
     9601 gacccggttc taactgaaaa tctttttact cgtattattc ttctggaaaa aggaagatta
     9661 accaagataa aatgaataaa ctccatcctc taagttttat tatcgctgct ctgtggttca
     9721 gtaccgctgc tatttttgtt cctcaattac cttggttaac tcttgctttt ttactttgtg
     9781 ctattttccg gggattactt actggtttta attttcggct ctggtggaag caagtaagaa
     9841 aaatgattcc ccttttcctc gctattctgg taatacaatt gctctttact aaaaaaggag
     9901 agttactctg gggaaaatcc tggtatgcta tatattctgt aggcctacat cgtggtattg
     9961 cggtaagtct tcggttactg attctatttt tctccgctca gatacttttg cgtttgaatt
    10021 atgaagattt tgatattgcc tttaacactc ttcatcttcc cgaagaattt gcctttatga
    10081 ttttttatac tattcatatt atcccttctg ctggagaaca tattcagcat tctttacaac
    10141 tggttaagct cagaggaata aatatgagga aactaccatt gaaatcacaa ctaaccatat
    10201 atcgttatat atctctcagt gttctggctg atgtgctttc acgcagcgaa attcaggcta
    10261 ttgcattaga cttgagagga tttcgctcta ctggaaaaca tactcatctt ctgcagaaaa
    10321 aattctctct ctatgacctt atgctttacc tatctattac tatcatcaca ctcgtatatc
    10381 tattttcaat attagcttga aacactattt tatatagctt atccgtctgg ttataattct
    10441 atcggattgt ttcaacctca gtatatatat tcccgaagga agcactttcc cattatcatc
    10501 tttcccgtca aaattaaaag tataatctcc tttcatcttt gcttcctgga agaaggtttt
    10561 aactttttga cccttaagat tatagagcga aagacttgct aatcccttat ccgctaagga
    10621 aatctggata tggcaatctt tatggaaagg attgggataa atcttcattg aagctaccac
    10681 tgaaggaata gtggaatcta ttactggtgt gttaatcaat tcagggatga ttagttgtaa
    10741 aaaatctcgc actccttctg cttgcatata ataaagagga aaacccagta atatcaattc
    10801 gccattatta cagctatgtc gtaaagccag acaatttcca ttatggggaa tagctggatt
    10861 catattagca atataaatta tctgttcagc ctcaggaaaa gtgcaaatat aaggcagcat
    10921 cccattccag gctggaatga gcttatttga atctacccaa agttctggat agatagtgga
    10981 ttgtgcactg atcagacagg gaacattatc ataaacaatc tgaggaatgc cagcaaatct
    11041 ttgaagaaaa gtttcggtta aaactatggc tgttttccaa cctgatatca atactttccc
    11101 tcctcctaaa atataacttc ccagaatact ttgatgctca accagtaagt tttctgccaa
    11161 atcatcagcg tgccagagaa ctactttata tccacccaga gttgctaaat ctggtaaacc
    11221 ttcacttgct acatcccagg aatcatattc tatgggagtt aaagctgcat tataaaaatc
    11281 atccaccatc gcatcatcag gactgatatt gctcccatta ccatctcggg tctcatccac
    11341 taccaaaaaa tcctgagcaa aagaaaatag gacgggaatt gcactcatta tattagattg
    11401 taaactgctc cagccttcac tatctatagc agatatagca tattggtata aagttccatt
    11461 aatagcggta ttatcaataa aacttaagct gtatagcggt tctgaattta aagctatcca
    11521 ctcactgcca ttatctacat tacgacgata aatccggtag cccaaatctg gactatctgt
    11581 aaaactttcc caagaaagaa acacagaatt attggtaggc aaacattcca ccagaaccgg
    11641 tcttttttct gtgattgaac gaagaagagt ccagttatga tagttaggat tgaatactgc
    11701 tcctgccccg agtgttatat tgaaataatt gttaaaacct tcaggtgaag cttgcacaaa
    11761 taaagaatct gaaattcctg cttgagttaa acggaatggt acctcaagat aaaaaggagt
    11821 actcgtatta gaaatcgttt tggcaactat ctttaagggt gaattactct cattattgaa
    11881 ccagacacta tattccacgg atggtattcc agggctatat atccattgct ggaagaattg
    11941 gtctaaatcc tgtccactta tttgttctgc catagcttga aattctgagg ttattgcatt
    12001 cccataacaa taggtgttat accagttctg gagcaaggaa aagaaatttg catctccaat
    12061 ctttaatctc aacatatgca gaacagatgc tgctttttca taggagggcg gagcaaaata
    12121 ggaattaaag ttcggattat aaatagtgtg tggtccctct gtattttccc aatttatata
    12181 ataattatga aattctgttc ttatgtagtt acaggcagat tcccaaccct ctgttttatc
    12241 tttccagaga tgttctgaat aggtagcaaa accttcagaa agccatacat ccttgaaagt
    12301 aagaaaggaa actgcatctc cgtaccattg atgtgccagt tcatgagcta tggtcgtttc
    12361 ataggcaaga gtcccattaa taatataatt ccccagagta gtcattgttt gatgttccat
    12421 agcaccgtaa gtgctcatat tgactaccgc atgaccatac ttctcaaagg gataaggtcc
    12481 aaaaagctga gaaaaatagt ctatcatcat cggaacccgg gataatgtta ttaaagcatt
    12541 gttatactga ccgggtaaga caaaattaat aataggaagt tcttccattg attgatcaaa
    12601 ttccacatag ggtccgcagg taacacaaac cagataggta gtcataggat attgacctat
    12661 ccagtaggta gtagaagttc catctccatt atctactata ttagttctca atccattagc
    12721 ggcaactttc caatcactcc tgatagtaat agttagatta attatagctt tatcccaggg
    12781 atggtcataa cagggccacc agtaacgagc tgcatctgga tctgaaatgg taaaaaccga
    12841 gtttgcagta aatatcatac caatatgata aatatcagaa ctcaattgcg gaatacctga
    12901 ataaaatacc tgcgtggtaa aagtagaacc agctgaaata ttcaggttta tatgaataat
    12961 gccattacta tgagtgtaag tagtaggaat attattcacc agaacattat taaccgtcaa
    13021 acctaccaga ttatattcaa tatagctcag attttcttcc gccagaaccg tcgctaaaac
    13081 atttccattg atataatgcg tttgttcatt tatggaaaga gtgagctcat acatttgcac
    13141 atcaaaacca gttaaagaat cagctttatc cgattctgac cagatatgat taacactata
    13201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tgtctaagtt ttatccacca
    13321 ctgaattctt ttcgctatct ctttttcaaa tccaaactct ccagggatat aataggtttt
    13381 ctcttccatc tgctcgggaa aatacttttg atatgcatat ttattgtcaa aatcgtggtc
    13441 atacttataa cctgctccat agccaaggtc tttcataagt ttagtgggag cattacgaat
    13501 atggaaagga actccataat gactggtttt tctggcatct tcagcagcag cttcataagc
    13561 ggtgtagaga gaattacttt tgggagcagt ggcaagatag actaccaatt gagccagagc
    13621 ggtgcttgct tcaggcattc caataaaaag aaccgcttcc tttacagcaa tagcttgaac
    13681 cagcgcttga ggatccgcta atcccacatc ttctgaggca aagcgaatca atcttcgcac
    13741 tatatagcga gggtcttctc ccgcttccag cattcttgcc agccagtaaa gacctgcttg
    13801 agggtctgat cctctaagtg atttatgtaa ggcagaaata aggttgtagt gttcttcccg
    13861 gtccttatca tagtaaagag tcttcttttc caggatagtt gccagttgtt ttatctcttt
    13921 atgtggttct tttttaagat aaggtgccag aagttccagc tgattaagtg ctctacgagc
    13981 atcacccgaa ctttgttgag ccagccatcc tattatatct tcatcttctt ccagtcctaa
    14041 actctcatat cctttgcgaa tgatagtttt taagtcctcc tctgaaagca tctgaagcac
    14101 aaaggtatga caccgcgaaa gaagcgctgg aataacttca aaagaaggat tctctgtggt
    14161 ggcacctata agtactatag caccagattc tacataagga agaaatgcat cctgttgagc
    14221 tttgttaaaa cgatgtatct catcaataaa gactatggta gtctgatttt gatgacgatg
    14281 catataatca gcatctttca ttactgcttt gacatctgtt atactggaaa ggacagcact
    14341 gaatctaaca aagcggtgtt ttgtgctgtt ttcaatgatt ctggcaatgg ttgtttttcc
    14401 actgccaggt ggaccccata aaatgaaaga ttgatattca tcactttcta tcatcttacg
    14461 caaaggagaa tcaggagctg ttagagcagg ttgtcctaaa acttcatcca gagaacgagg
    14521 acgaagtttc tctgctaaag ggacatttct atccgtaatt tctttttctt ccagaaaatc
    14581 cgtctgatca gaattcattg gttaaaaatc cataaaacca tccagctcgc tgggatcaaa
    14641 ccattccagt gcaccaagta tcctttgcat ttcggtcaga agcaaggtct tgtgttgttc
    14701 ttcttcttta gctaaatgga gcaaaagttc tttaatgtcc actggttcag tctgttctgc
    14761 aaaagaataa taattcttag ctgcctcttc ctcacgagtt atagcaaact ggagaagttg
    14821 agcgggatct tttaaatcca cagatggaat ctcttttatc tcctgagttt caggttcagg
    14881 ctcttcacca gggaattcct ggctgaaagc acttctcaat ttgagttcat gctctttttc
    14941 taaaccgacc aattccgtga aaaaggcatt ggtttcaggc tcgccaaagc ttctggctaa
    15001 gccctgataa agcctttgag agcgttgttc ctgagctatg gcaaaattgt aaagttcttt
    15061 tctatccatc ttttgtattc ctttatgccg tctggcgtat taattctact ggatttatat
    15121 tctttaattt tcttaaaggt aaaataacac ttaaaaaagt gaccacatac gaaaatataa
    15181 ttaccataag ataatctgaa gtggcaaatt tcacgggcaa agcagataaa cctgccgttc
    15241 ctaaatccat acttatgata ccatattgct gctgaattaa caagagaaca gtagcaatga
    15301 ttaatcccag cgcaattccg agagtggcta aaaacaaagc ctgatataga aaaagggtgc
    15361 ccaattcact ttccttataa ccaaaagcct tcagtaatcc caattccttc ttcttgcgag
    15421 tgatggtctt ccataaatta cctatcaaat taaaactggc tataatatac ataaatagca
    15481 taatcaagaa cataagattt ttctcaaatc tgatagaact gtataaactg ggatcatagg
    15541 cactccaatc ctccacttca aactctggaa ataaagcttt tattttcctg gtagcgcgtt
    15601 gaggattaga aaaagcaccg gttttgacct ctatataatc aatctcattg gtgtaaccac
    15661 tgaaaaattg agctatagat aaagggataa aactataggt ttcatcgtat tctggcatcc
    15721 cagtggtaaa aatagcagta acctttaatt ttctgactcg cggtaacatc ccaaaagcag
    15781 aaggcacatt aaaaaccgga gataaaagct ctatctcatc accaatgcga acatccagac
    15841 ttgctgccaa gccaattccc atagctatgc caccatctgt aaactcatta ctctgaatat
    15901 taccggctaa taatccctga aatccagcag aattatccat ttctttgggc tggaacacag
    15961 aactgatttt agaatgtcta ttcgggtcta ttcccagaga aagtgttgga gaaagctttc
    16021 cttcttttat tatcaccaat tcatttcgca ctactggggc tgataaaaaa ccttcttgat
    16081 tcaacttatc taccatctta tcatattctg aaaaaggtgc tccatctggt ctggaaatct
    16141 taatttcact taaagttcca gttattctct cctgtatatc cgttcttaat ccattcatca
    16201 cactactcac acataggaga cctgttactc caagggttaa acctatcaaa gttagaaaat
    16261 gagacctatt tatgaaaaaa gcactttgcc ctctaagata tctcttagcc agccaaatta
    16321 tacttctatt tttctgtttc ataaactcta ttttaatttc cctaatacaa aagtcaagag
    16381 ctttcaatat gttaaatatt ttgctttact ttagaaccta tccttcttga tggttaatga
    16441 cctccttttt catctcttca ccatatccaa ataacctcta tttcattcct attttctttt
    16501 ttctcccctc atctctaaat tatatccgat aattaactaa cttttatgga gcttatgtcc
    16561 aattttggac ataagctgga cataagctgg acataagctg gacataaatt gttcataatc
    16621 tcaccataaa aaggtagaaa gaatgaaaaa atgtagagtc tt
//
LOCUS       DAORZF010000038        16444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_2866_length_16444_cov_388.032644, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000038 DAORZF010000000
VERSION     DAORZF010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 16444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16444
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2866_length_16444_cov_388.032644"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="PK015_05415"
     CDS             complement(<1..545)
                     /locus_tag="PK015_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33940.1"
                     /translation="MNQEEIQFHPYSPFIPETTRRLILGSAPPSRFCSPNKTELKDKD
                     IDYYYGSYSRGYNLLWDLLFHIFEPESLSELKNIRNLQIPREERTCTQQKFFQNFLQR
                     YYLGIADILYQFIRRDQSSADSNIRPLKFLNLTEYLYHSAELQTIFCTSHYKVFIWLK
                     QYLHTQDINLLKDAEDFYFLLP"
     gene            complement(732..1184)
                     /gene="purE"
                     /locus_tag="PK015_05420"
     CDS             complement(732..1184)
                     /gene="purE"
                     /locus_tag="PK015_05420"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOL33941.1"
                     /translation="MPQIRIILGSKSDVEQCEAMQQILKDFGVDYDFHISSAHRNPEK
                     TAQLAKNAEAEGVQVIIAAAGMAAHLPGVIASHTILPVIGVPLVSGNLGGIDALLSIV
                     QMPTGVPVATVAIGGVKNAALLAIQILALNNPELKEKLRIYRNQLALQ"
     gene            complement(1187..1861)
                     /locus_tag="PK015_05425"
     CDS             complement(1187..1861)
                     /locus_tag="PK015_05425"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOL33942.1"
                     /translation="MAKRYISQAEAAKKLKVSEKVIQEMINSKTFETKMIGKTLKIDE
                     DSLNEWLENLNESEEKMLALKRVTCHFEEYMRPENIFLDFEAENKYDAIRILSEKAKE
                     LKLVRDARWLYEVVVAREELISTAIGHGVALLHPRHLHPSKIKTPSILFGRSKNPVDF
                     DAPDNQPVNLFFMLLLHNDKQHLFSLSYISKLIMNPEILEALSTATTPEEIHRCLTVL
                     PEEKNK"
     gene            2040..2453
                     /locus_tag="PK015_05430"
     CDS             2040..2453
                     /locus_tag="PK015_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HOL33943.1"
                     /translation="MKKVAIRPFENKSTEFELGDIVYNGLVKEFQKDGRLKLVTQQPD
                     CVVEGTILKLEEDVYSYDSWNNVQDYMLRLTCSVVFTDLINNQIIYENKSLVITEPYA
                     VSPASTAKCRSKEEAIDELVSKLFRNIIQNSLETW"
     gene            2395..3183
                     /locus_tag="PK015_05435"
     CDS             2395..3183
                     /locus_tag="PK015_05435"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOL33944.1"
                     /translation="MSSSLSSSETSSRTALKPGDTIRLNHFLAKSGICSRREADNLIK
                     SGQISVNGEICTDLGCQIDPIKDIIIYKGKKVQPQKDKVYLMLNKPKGYLVTHSDEFN
                     RKTIYELLPDNAQDLRYAGRLDKDSEGLLLLTNDGELLNLLTHPSSKTEKVYKVRINR
                     KLTKRELNILRQGVPIEGGKTQPAGVFVKNSSENDMTLKIVLTEGKKREIRQMMEAVG
                     AKVLSLRRVQFANLRLKDLPIGRWRYLTRAEIKALNDSVEKASR"
     gene            3180..4256
                     /locus_tag="PK015_05440"
     CDS             3180..4256
                     /locus_tag="PK015_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HOL33945.1"
                     /translation="MKIAIIGPPKTGKTTIFNALTGNSVSTDKYAPVANEPNVAVVQV
                     PDERVTKLSELYQPKKTIYAQIEYRDYPGIFSASNENPDNAMFSDIKTCDGFVLVMRA
                     FVDEELEELYPLGEVLHQLTNFEEEMLLMDLMIAEKRREKIELGYKRGVKTPALQFED
                     KVLEEVCAQLQAGKPLREMQLNAEEEKVIRGFKFFTQKPMIVLINCNEEDYHNLDNLK
                     SEIQAKGYTAEVIAGRFEEELSKLDNEEAKLFMEDIGIEESIKDRLTRLCYSMLGYIS
                     FFTVGEDEVRAWTIEKGSNAVTAAGKIHSDLARGFIRAECFNYNDLMEYGSEKHLREK
                     GLFRLEGKDYIVQDGDILFIRFNV"
     gene            4271..>5439
                     /locus_tag="PK015_05445"
     CDS             4271..>5439
                     /locus_tag="PK015_05445"
                     /inference="COORDINATES: protein motif:HMM:NF036827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOL33946.1"
                     /translation="MNNNRKNSRNHKKGMKTWQKRLIDGLVLIFSVLVVISLIYGLDS
                     ILNDVDALSLHGNVFTWPFQKEVQVENPIGVFGVAVGYAFSYLLGYYFSFIGFVLLAI
                     MSLLNFLEPAKKRNYHKFYLILIASFLLQVILVKGLRDYSHSVLPRAIWAGLSAIFSN
                     FGASLLIILGIVLCLILIFEYERLKLWFSKVRDSISENRKKASLKKKKDKLTINEKEV
                     HPPVIPVIQNHIANIPKEETATPKKEEIPEPELISPIIEVFGPPSSMDKPPYVIPSVE
                     DFLESPVKLSERDRKEIENQILATSQILKNKLAEFGIEAEVRNVNIGPIITQYELEPA
                     KGVKVNRFTSLADDLALAIKAKSIRVQAPIPGRGLIGIEIPNMTRDMIYLKDLLLS"
     assembly_gap    5440..5539
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5540..6601
                     /locus_tag="PK015_05450"
     CDS             <5540..6601
                     /locus_tag="PK015_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HOL33947.1"
                     /translation="AIDSKLAFGLGKDIAGRPVVSDLAKMPHLLIAGATGSGKSVCIN
                     TIIMSFLFRTTPEDLRLILIDPKRVELTGYNDLPHLLGSVVTDSESALENLYWAVKEM
                     ERRYELLHDAGVRDINAYNEKATQEGELKKLPYIVIIIDEFADLILTSGKDIELPITR
                     LAQMARAVGIHLILATQRPSIKVITGIIKANFSARIAFQVSSKVDSRVILDQIGAERL
                     LGNGDMLFLPPGKAQPERIHGAFVSDKEIARVCEFLKTQPKPEDDFVLPVQSSEEMGN
                     FEYDDELFPEAAKLVVRANTASVSMLQRHFRIGYARAGRLIDLLERAHIIGPHMGSKS
                     REVLATTEDLIRRGIIRDE"
     gene            6678..7295
                     /locus_tag="PK015_05455"
     CDS             6678..7295
                     /locus_tag="PK015_05455"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer-membrane lipoprotein carrier protein LolA"
                     /protein_id="HOL33948.1"
                     /translation="MKKAIVVSLLLIWSVMLLSITTGEVYNKVSSFYANLQSFQAKVQ
                     QTNYYAQLKKTITYKGTIYFTPGRMLMHFDDPTVQRLKIENGRIELYDASSNTLFKTL
                     MQPEYDKLNPVEILQSYWNKSKVSIISETKDRVNIELVPQNDDFIQSLSATLDKNNGL
                     IYKLSYKDKTGNSVTYNFSSIKLNQGIPSSVWKYDYPKNVQIIES"
     gene            7306..8361
                     /locus_tag="PK015_05460"
     CDS             7306..8361
                     /locus_tag="PK015_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HOL33949.1"
                     /translation="MIALIMAGGSGTRFWPLSRASLPKQYLRILGDKSMLQLTFERLE
                     TLVPPEKVYVVTSSSQAILVKEHLPELPPENIIIEPFGMNTAPCIALSVEYLSALYNE
                     NETMVVLPADHIIKDTDTFIAVLKKAETAAQKGMLITFGIVPDYPATGYGYIEKGEEL
                     EEGIFSVKQFKEKPELDTAKEFLRQGTFFWNSGIFCWTLSSIHKALKEFLPETLTLAE
                     EVLQLKQSFASEEAIAAVYRKMPRIPIDIGVMEKARFRAVIPVSFGWSDVGSWKALAD
                     ISPKDENQNTFKVDGFALNARDDYVYSDKFVALIGVDGLCIIETDDALLVTSKEQSEK
                     VKEVVEYLQNNHKDNLL"
     gene            8468..10321
                     /locus_tag="PK015_05465"
     CDS             8468..10321
                     /locus_tag="PK015_05465"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOL33950.1"
                     /translation="MQNRNRILMLIFLMIVAAGICSAQKVQFNISPAQLHPGEKGVLQ
                     ATLIIQDSDKKQSYDPSEGENGYLTLKVIGSYPQLNFESTKYPEPDKITDGIWEYYGK
                     FTLKKPFVVKADAKPGKLSIKVELQYGLCHKSSGFCDPPQTVEGTVQLEILPTATLAE
                     SENEQFIEKEQDTLATNASVSTKEEIPSAPVQKEESGNILIYMLLAILGGIVLNFTPC
                     VLPILPIRAMSMINQAQKDITKLMIHTLLYTLGVLLSFGAIAAVFVIARASGVNLTYG
                     FLSQSVTYNLIMMSILFLFGLSLLNVWEMTLPGMNAAAKATSKGGYIGSFFNGVFAFL
                     MGFSCMGPFMGPALEVAFRLSSPMLVLFFLLIGFGFALPFIIISLFPKALKALPKPGE
                     WMNIFKEAMGFVLLWLTWKYVSNIWSLTKSGPFLINTLLFIFWMGVAAWLYGRFVRPQ
                     YKKWVQFLLGFAAIAIIIWSASYYLKWEPEKEANKQEQVQVEGMQPAEQEGWYVFTPE
                     LFYKLQEEGKPVYVEIGADWCTNCKTNERKVLNQEDIIAEFERRGVIRLKGDFTRENP
                     VLKDFMLQYGSIGVPFNMLFIPGEEPIKMPELFSKSDLKKALEHIPVKEKQ"
     gene            10318..11448
                     /locus_tag="PK015_05470"
     CDS             10318..11448
                     /locus_tag="PK015_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33951.1"
                     /translation="MKRNLLLLITLISILFVTSCDRFTHNFQPPVTVNLETELFSPLE
                     DILNSQSENMIDNVMQFYAEDYLYYGLQKEDIRNWLQSIYSVAPEAQAQVTLISSEML
                     SETTALADWRLLITTDRESTVLVDSLFYSEHIAQREGKWLLRGNQLSGSYPSTKEHII
                     IEYFTFLTCPNCPVVENKLNKWQDAYPQRISYLEHHISDQLAIPGDEVYQYYGYPPFP
                     SSIIQGEMALMGNSPAILAEYDTAIQSLLEKDADILYLYPVIRINNKQVTGSITLQLL
                     NPELDLTNLYLNYALIDKVSPFQNTNYDYVRNVVLAQGSKTLTSSDLEGPISFSLTSS
                     ITIPNDAAIVIFAQTKPPVFENNAKIHSGIEIALDHIIKSKK"
     gene            11464..12000
                     /locus_tag="PK015_05475"
     CDS             11464..12000
                     /locus_tag="PK015_05475"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOL33952.1"
                     /translation="MKKLTILTLLLLLVAILMAEPMPDFRLPDESGKNVSLSELLGKG
                     PIIIDFWADYCQPCKQAMPALQELVNKYDSLTVVLISLDAPKAQPKAKAYLKSKNYSF
                     ITLFDPDKTLAKKLNVENPPHTFILDKTGEIVYSHLGYEPGVEKEYEHKVRKLLGLAN
                     EEGEECTCTEKCPDCQQK"
     gene            11976..13427
                     /locus_tag="PK015_05480"
     CDS             11976..13427
                     /locus_tag="PK015_05480"
                     /inference="COORDINATES: protein motif:HMM:NF039629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6029 family protein"
                     /protein_id="HOL33953.1"
                     /translation="MSRLSAKVILLPLIFLAGITFIPAQTTLTVNGLNQTSFVYRAAS
                     DSLNCYFYDSFAFNLGYKDFSFGMKFIAELPKYSIEQSELLAELNPDRLNLGWKELYA
                     SYNRNNYQVYAGIMEETFGSGLIFRSYQDLEMDDDYRVNGFRFSYDNKLRVKALYSAY
                     SNPINRGKLDLAYGADLQYPILKPLTLGVTALSLQSYIGSSYKEDNILGGRVSFQEDW
                     IDGSIELAQRQKAYSNDNGSAVYGNLSTYFGPLQIGGAYKYYDSFDYFNHLQDIPLAN
                     HHNETLADNQSSGLDEEGLQGWMSFAFLKYWTFNLDYAEAWNHNHKVKMNDFFVSLDW
                     NKDLKMASISYSQVEKIDEILSHWQKESYPGFNLQFPGKKNPFTLSGEFKVVEKQVYD
                     VETSHYEPKIQFDTSFNKLSCSLALQSWWKDFSHLTQSRYMPSVQVKYPLFSETDILL
                     LVGKEAGGKVCRNGICRYVAPFSGIKLEMTTRF"
     gene            13441..14769
                     /locus_tag="PK015_05485"
     CDS             13441..14769
                     /locus_tag="PK015_05485"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOL33954.1"
                     /translation="MKKTLVLILLMSVALLAANPNYFPRTSLAELCLIQDNSDCNEAM
                     MQLNEVLDNTNRGEFIATHLFQNDNNLGNVSVNERINDLGVEILPTIFFNRESSVTGV
                     PGTGIYNNIVNSYKFSASPLKMNITSFNTETGAISVTVKMLDPELSLINQKLILILAQ
                     NSMGTYNNVARQYLYHNINLTGPGEEVTINDNFSYDASVEPDNIWAIALVEVDNQVIL
                     QSTSTLPLPEYYLRCAYDWEGNNIIVDPNSNFLSEPLWIFNLGAADTFNMRIVVDDAP
                     ANWYFNYCDEEGNCYPGGISMPLNLSSGQVKGFHLNLMVGSTGTAHFHFELTSPNLGT
                     YNIPFICHTSDPIQDDIIQVPAVMTLKANSPNPFKNMTSFSISANKAGYATIQIFNLK
                     GELVAETNSHSIYQGENKIEWKVPSRLANGIYFYRLKEQPASLAKMLLLK"
     gene            14810..15541
                     /locus_tag="PK015_05490"
     CDS             14810..15541
                     /locus_tag="PK015_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HOL33955.1"
                     /translation="MKFVDRFLTRTEQKAILAICGLALLGFILGQFDGFHNLLAKKTA
                     TPETTEELVKAVKEDKPVQIDIRTASKEELILLPGIGEKRAQDIIDYRNQYGFKSTDD
                     LLQIKGIGEKTLAKMLPSLLIFGNPEFTPEKIPASGQDTSQIAISDKGKNTDKTSTNQ
                     KATDAKKKTSSASVPKSQLTNIVNINTAGIEELCTLPGIGEVKAQAIIDYRNQNGKFN
                     AVEDILNVKGIGPKTLEKIRSRIKV"
BASE COUNT         5219 a         2789 c         3214 g         5122 t
ORIGIN      
        1 ggtagaagaa aataaaaatc ttctgcatct tttaggagat taatgtcttg ggtgtgaaga
       61 tattgtttta accagatgaa aaccttataa tgactggtgc aaaagatagt ttgaagttcg
      121 gctgaatgat atagatattc tgtcaaattt aggaatttca aaggacggat attactgtct
      181 gcagaactct ggtcacggcg gataaattga taaagaatgt cggctatacc taaataatac
      241 cgctgtaaaa agttctggaa gaatttttgt tgagtgcagg ttcgttcttc tctcggaatt
      301 tgcagattac ggatgttttt aagttcagat agagattcag gttcaaaaat atgaaaaagt
      361 aaatcccaga gcagattata tccccgactg taacttccgt aatagtagtc tatatcttta
      421 tctttaagtt cggttttatt aggagaacaa aagcgagaag gtggtgcact tcccagaatc
      481 aatcttctgg tagtttcagg aataaaaggt gaatagggat gaaattgtat ctcttcctga
      541 ttcaatttaa tctcctatta tctgtcattt aatgtagata aagataggtt tttttttgct
      601 atattattca gtagatagcc aataatcctg taataaagta atccaatgcc gctttttcag
      661 aaagattaat cctcttgaag aatacttact ttctgactct atcttaaata ttaaaaatga
      721 agggattaat cttactgcag agcaagctga tttctataga ttctcagctt ttccttaagt
      781 tcaggattgt ttaatgccag aatctgaatt gccagcaagg ctgcattttt tactccacca
      841 atagcaactg tcgctacagg aacacctgtt ggcatttgaa caatggaaag taatgcatct
      901 ataccaccca aattaccact aactaagggg actcctatca ccggaagaat ggtatgtgaa
      961 gcaattacac cgggcaaatg agctgccatt cctgctgctg caataatgac ctgcacacct
     1021 tctgcttcgg catttttagc cagctgagct gttttttcag gatttctatg ggcggaagaa
     1081 atgtgaaaat cataatccac accgaaatcc ttcagaattt gctgcatagc ctcgcattgt
     1141 tctacatcgc ttttactacc cagaattatc cgaatttgtg gcataattat ttattctttt
     1201 cttctggtaa aacagttaag cagcgatgta tttcctcagg agtagtagca gtggataaag
     1261 cttccaagat ttcaggattc attattagtt tggagatata ggaaaggctg aatagatgct
     1321 gtttatcatt atgcagaagc aacataaaga aaaggtttac aggttgatta tcaggagcat
     1381 caaaatcaac gggattcttt gatctgccaa acaggatact gggagtttta atcttggaag
     1441 gatgcagatg acggggatga agtaaggcga caccatgtcc aatagcagta gaaatcagct
     1501 cttctcttgc tactacaacc tcataaagcc agcgtgcatc acgcacgagt ttcagttcct
     1561 tagctttttc actcaggatt cggatagcgt catatttgtt ttccgcttca aaatccagga
     1621 aaatattttc tggacgcata tactcttcaa aatggcaggt aacccgtttt agagccagca
     1681 tcttttcttc tgattcatta agattttcca gccattcgtt tagagaatcc tcatcaatct
     1741 tgagggtttt tcctatcatc ttggtctcaa aggtcttact attaatcatt tcctgtatta
     1801 ccttttcgga gaccttcaat ttcttggcag cttcagcttg cgatatatat cgttttgcca
     1861 tggctaacct cccttattgt atttattttt tactatataa tataatccag gtttatcttg
     1921 acaagtataa agcaattttt gaaaaaggaa taatggtcaa gaaatttatt tatttgggac
     1981 tggttgcagc agtcctctgt ggctgctcat attccgtata ttcaaatgct tatccgcatt
     2041 tgaaaaaagt tgctattcgt ccttttgaga ataagtctac ggaatttgaa ttaggtgata
     2101 ttgtctataa tggattagtt aaagaatttc aaaaagatgg aagattgaag ctggttaccc
     2161 aacaaccaga ttgtgtagta gaaggaacca ttttaaaatt ggaagaagat gtctatagtt
     2221 atgatagttg gaataatgtt caagactata tgttacgatt aacttgtagt gtagtcttta
     2281 cagatttgat aaataatcag attatctatg agaataaaag tctggtgata acagagccct
     2341 atgctgtatc acctgccagt acggctaaat gtagaagtaa agaggaagca atagatgagc
     2401 tcgtctctaa gctcttcaga aacatcatcc agaacagcct tgaaacctgg tgatactatt
     2461 cgtcttaatc attttttggc aaaatccggc atctgttctc ggagagaagc tgacaacctg
     2521 ataaaatctg gtcaaatctc tgttaatgga gaaatttgta ctgatttagg ctgtcaaata
     2581 gacccgataa aagacatcat tatctataaa ggtaaaaaag ttcaaccaca aaaagataaa
     2641 gtgtatctaa tgctcaataa accgaagggt tatctggtta cgcattctga tgaatttaat
     2701 agaaaaacca tctatgagct cttgccagat aatgcccagg accttcgtta tgcaggaaga
     2761 ttggataagg attcggaagg attgctgtta ttaaccaatg atggagaact gctaaattta
     2821 ctcactcatc cttcttctaa gacagaaaag gtttataagg ttagaattaa tagaaaattg
     2881 actaaaagag agctgaacat tctaagacag ggagtcccta ttgaaggcgg aaagactcag
     2941 ccagctgggg tttttgtaaa aaatagttcc gaaaatgata tgactttaaa aatagtatta
     3001 acggaaggga aaaaacggga aatccgtcag atgatggaag ctgtaggtgc aaaagttttg
     3061 agtctgagaa gagttcaatt tgccaatctg aggctaaaag atttgcctat aggacgctgg
     3121 agatacttga ccagagccga aataaaagcc ttaaatgatt ctgtggagaa agcttcaaga
     3181 tgaaaatagc tattattgga cctcctaaaa ctggtaaaac taccatcttt aatgccttaa
     3241 caggaaattc tgtttccact gataaatatg cgccagtagc aaatgaaccc aatgtggctg
     3301 tagttcaagt tccggatgaa agagtaacta aattaagtga actttatcaa cctaagaaga
     3361 ccatttatgc tcagatagag taccgggatt atcctggcat tttttctgct tctaatgaaa
     3421 atccggataa tgcgatgttt tctgatatta aaacttgcga tggatttgtc ctggtaatgc
     3481 gagcttttgt agatgaagaa ttagaagaac tctatcctct gggagaggtc ttacatcaat
     3541 tgactaattt tgaggaagaa atgctcctga tggatttgat gatagcagaa aaaaggagag
     3601 aaaagattga actgggctat aaaagaggcg taaaaactcc cgctctccaa tttgaagata
     3661 aagttttaga agaggtttgt gctcaacttc aagcaggaaa acctttacga gaaatgcaac
     3721 tcaatgcaga agaggaaaaa gttattcgag gtttcaaatt cttcacgcaa aaaccgatga
     3781 ttgtgcttat aaattgcaac gaagaagatt atcataattt agataattta aaatcagaaa
     3841 tccaagctaa aggatatacg gcagaagtta ttgcaggaag atttgaagag gaattgagta
     3901 aactggataa cgaagaagca aaacttttta tggaagatat aggtattgaa gaaagtataa
     3961 aagaccgttt aactcgttta tgctattcta tgttaggtta tatcagtttc tttaccgtag
     4021 gtgaagatga ggtgcgtgct tggactatag aaaaaggtag caatgctgta actgctgcag
     4081 gaaagataca ttcggattta gcacgagggt ttattagagc agaatgcttt aactataatg
     4141 acctgatgga atatggttcg gaaaaacatt tgagagaaaa agggcttttc cgactggagg
     4201 gtaaggatta tattgttcag gatggagata ttcttttcat tcggttcaat gtttgagaga
     4261 ggattaattt atgaataata ataggaaaaa ctctcgcaat cataaaaaag gaatgaaaac
     4321 ctggcagaaa cgtttaattg atggtctggt gcttatcttt tcagttttag tagttatcag
     4381 tctgatctat ggtttagatt ccattctaaa tgatgtagat gccttaagct tacacgggaa
     4441 tgtttttacc tggccctttc agaaagaggt gcaggtggaa aatcccatag gtgtctttgg
     4501 agttgcggta ggctatgcat ttagctatct tctgggatat tatttttctt ttataggttt
     4561 tgtgttgctc gcgataatgt ccttactcaa ttttctggaa ccagccaaaa aaagaaacta
     4621 ccataagttt tatttgatac ttattgcaag ctttcttttg caggtgattt tagttaaagg
     4681 tctaagagat tattctcata gtgttcttcc tcgtgctatc tgggcaggtt tatccgctat
     4741 attcagtaat tttggtgctt ctttattaat tattttgggg atagttttat gcctaatcct
     4801 tatttttgaa tacgaacgat taaagctctg gttttccaaa gtgcgtgata gtatctcgga
     4861 aaatcgtaag aaagcctctt taaaaaagaa aaaagataaa ctgactatta atgaaaagga
     4921 agttcatcct ccggttatac ctgttattca gaatcatata gcaaatatac ctaaagaaga
     4981 aacagccact cctaaaaagg aagaaattcc agaaccggaa ctgatttcac ccataataga
     5041 agtttttggt cccccttcca gtatggataa accaccctat gttattcctt cggtggaaga
     5101 ttttttggaa agtccagtta aactatcaga aagagatcgg aaagagatag aaaatcagat
     5161 tttagcgacc agccagatac tgaaaaataa gttagcagaa tttggtatag aagcagaggt
     5221 ccgtaatgtt aatattggac ctataatcac tcagtatgaa ttagaacctg ctaagggtgt
     5281 gaaggttaat cgtttcactt ctctggcaga tgaccttgct ttagctatta aggcaaaatc
     5341 tattagggtg caagcaccta ttcctggaag aggacttatc ggaattgaaa ttcctaatat
     5401 gactcgcgat atgatttatc tgaaggattt acttctttcn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnng caattgattc taagctggct tttggcttgg ggaaagatat
     5581 tgctggaaga ccagtagtta gtgacttagc aaagatgccg catcttttga ttgctggtgc
     5641 aacaggaagt ggtaagagtg tgtgtataaa caccattatt atgagttttc tctttcgcac
     5701 cactcctgaa gatttaaggc ttattctgat tgaccccaaa agagtagagc ttactggata
     5761 taatgatttg cctcatcttc ttggttccgt ggttactgat tcagaaagtg ctctggagaa
     5821 tctatattgg gcagttaaag aaatggaaag acgttatgaa ttgcttcacg atgcaggtgt
     5881 gcgtgatatc aatgcctaca acgaaaaagc aactcaagaa ggtgaactga agaaactacc
     5941 ctatattgtc ataatcatag atgaatttgc cgatttgatt cttaccagtg gcaaagatat
     6001 tgaattacct atcaccagat tagcacaaat ggctcgtgcc gtaggaatac atttgattct
     6061 ggctactcaa agaccttcca ttaaggttat aaccggaatt attaaggcta atttttctgc
     6121 tcgtattgct tttcaggttt cctccaaagt tgattctcgc gttatcttag accaaattgg
     6181 agcggaaaga cttttaggga atggagatat gctattttta cctccaggaa aagcacaacc
     6241 ggaacgcata cacggtgctt ttgtatctga taaagaaatt gctcgggttt gtgaatttct
     6301 taaaactcag cctaaacctg aagatgattt tgtcttacct gttcaatcca gtgaggagat
     6361 gggcaatttt gaatatgatg atgagctctt tcctgaagcg gcaaaattgg tggttagagc
     6421 gaataccgct tcagtttcaa tgctgcaaag acactttaga ataggttatg ccagagccgg
     6481 aagattgata gaccttctgg aacgagctca tatcattgga ccacatatgg gttctaaatc
     6541 cagagaagtt ttggcaacaa ctgaagattt aatccgtaga ggaataatcc gggacgaata
     6601 agttttaagc ctatgtcagc ttttactaaa gtttatagtt atagatatca ttttaataag
     6661 gagttttcca aactataatg aagaaagcaa tagtagtttc tctcttgcta atctggagtg
     6721 tgatgctgct aagtatcact actggtgaag tctataacaa agttagttca ttctatgcta
     6781 atctccagtc ttttcaagct aaagtgcaac agactaatta ctatgctcaa ttaaaaaaaa
     6841 ctatcaccta taaaggaacc atttatttca ctcctggtag gatgttaatg cattttgacg
     6901 accctaccgt tcagagattg aagattgaga atggcaggat agagctttat gatgcttctt
     6961 ctaacacatt gtttaaaacc cttatgcaac ctgaatatga taaattgaat ccagttgaga
     7021 tactgcaaag ctactggaat aaatccaagg tatccataat ctcagaaacg aaggatagag
     7081 tgaatataga gcttgttccc cagaatgatg attttattca atcgctttct gccactttgg
     7141 ataaaaataa tggtttaatc tataaactga gttataaaga taaaacagga aattcagtta
     7201 cttacaattt ttcttctata aagttaaatc aaggtatacc ttcttctgtc tggaaatatg
     7261 attatcctaa aaatgttcaa ataatagagt cttgaggaga atgagatgat agcattgatt
     7321 atggctggag gttcaggtac cagattttgg ccccttagtc gtgcatcttt accaaaacaa
     7381 tatcttcgta ttcttgggga taaatcaatg ttgcagctaa cctttgagcg tctggaaacc
     7441 ttagttcctc cagaaaaggt ctatgtggtg acttcttctt ctcaagctat tctggtgaaa
     7501 gaacatcttc ctgaacttcc accagaaaat ataatcatag aaccttttgg gatgaatacg
     7561 gctccctgta tagccttgag tgtggaatat ttaagtgctc tttataatga aaatgagact
     7621 atggtagtgc ttccagcaga ccatatcatt aaagatacag atacctttat tgctgtctta
     7681 aagaaagctg aaactgctgc ccaaaaagga atgctgatta cttttggtat agttcctgat
     7741 tatccagcta ccggttatgg ctatattgaa aaaggagaag aactggaaga aggaatattt
     7801 tctgtgaaac aatttaaaga aaagccagaa ctggatacag ccaaagaatt tttgaggcag
     7861 ggaacttttt tctggaacag tggaatcttt tgctggacct taagttctat tcataaggct
     7921 ttgaaagagt ttcttcctga gacccttact ttggcagagg aagtcttgca attaaaacaa
     7981 tcctttgcct cagaagaagc aatagctgcg gtataccgca aaatgcctcg tatacctata
     8041 gacattggcg taatggaaaa agccagattc cgagcagtca ttccagtcag ttttggctgg
     8101 agtgatgtcg gttcctggaa agctctggcg gatatctctc ctaaagatga aaatcagaat
     8161 accttcaaag tggatggctt tgcccttaat gccagagacg attatgttta tagcgataag
     8221 tttgttgccc tcataggagt ggacggtctt tgtattatag aaacagatga tgcactattg
     8281 gtcacctcta aagagcaaag tgagaaggtc aaagaggtgg tggaatatct tcaaaataat
     8341 cataaagata acctgcttta aggaatattt atctttatga gaaggaattt agtggtattt
     8401 tgttttaggc tggattataa aatgctttaa tatttaacta aagtgaaata tcaaagcgag
     8461 agaaaatatg caaaacagaa atagaatctt aatgctaatc ttcctgatga tagtagcagc
     8521 tggaatatgt tctgctcaga aagttcaatt taatatcagt cctgctcaat tacatcctgg
     8581 ggaaaaaggc gttctgcaag ctactctaat aattcaagat agtgataaga agcaatctta
     8641 tgacccctcg gaaggtgaaa atggctatct aactctgaaa gtgattggtt cctatcctca
     8701 attgaatttt gaatcaacta aatatcctga acccgataag ataactgatg gtatctggga
     8761 atattatggc aaattcacac tcaagaaacc ttttgtagtt aaagctgatg ccaaaccagg
     8821 gaagctaagc attaaagtag aacttcagta tggactctgt cataaaagta gcggattttg
     8881 tgatcctcct caaactgtgg aaggaacagt tcaattggaa atccttccta ctgcaacttt
     8941 agctgaatca gaaaatgaac aatttataga aaaagagcag gatacccttg ctacaaatgc
     9001 ttctgtttca accaaagaag agattccttc agctccagtc caaaaagaag aaagtggaaa
     9061 tatccttatt tatatgttat tagccattct gggaggtata gttctaaact ttactccctg
     9121 tgtgcttcct attttaccta tcagagctat gagtatgatt aatcaagctc aaaaggatat
     9181 aactaaactg atgatacata ctctgcttta tacactggga gtgctactct cttttggtgc
     9241 gattgcagct gtttttgtga ttgccagggc ttcgggagtt aatcttacct atggattttt
     9301 aagtcaaagt gtaacctata atcttataat gatgagtatt cttttcctct ttggcttatc
     9361 tttattaaat gtctgggaga tgactttacc cggaatgaat gcggcagcaa aagctacttc
     9421 caagggggga tatatcggtt ctttctttaa tggagttttt gctttcttaa tgggtttttc
     9481 ctgtatggga ccctttatgg gacctgcatt ggaggtagca tttcgtttat cttctcctat
     9541 gctggtgcta tttttccttt tgattggttt tggttttgcc ttgcctttta taattataag
     9601 tcttttcccc aaagctctta aagcgcttcc taagcctggt gaatggatga atatttttaa
     9661 ggaagcaatg ggctttgtgc ttttatggct cacctggaaa tatgtgtcta atatctggag
     9721 tttaactaaa agtggtcctt ttttgataaa caccctgctt tttattttct ggatgggtgt
     9781 tgcagcttgg ctttatggaa ggtttgtccg tcctcaatac aagaaatggg tgcaattttt
     9841 attgggcttt gcggcaattg ctattattat ctggagtgct tcctactatc ttaaatggga
     9901 accagaaaag gaagctaata aacaagaaca agtgcaagtg gaaggaatgc aaccagcaga
     9961 acaggaaggc tggtatgttt tcactcccga acttttttat aagttgcaag aagaagggaa
    10021 accagtctat gtagaaatcg gtgctgactg gtgcaccaat tgcaagacta atgagaggaa
    10081 agtgctcaat caagaagata ttatagctga atttgaaaga cgcggggtga ttcgtttaaa
    10141 aggagatttc actcgtgaaa accctgtctt aaaagacttt atgttgcaat atggaagtat
    10201 aggagtgccc tttaatatgc tctttattcc aggggaagaa ccgattaaaa tgccagaatt
    10261 attcagtaaa tcagacctaa agaaggctct ggaacacatc ccagtaaagg agaagcaatg
    10321 aaaaggaatc tattgctcct gattacgctt atatcaatac tttttgttac cagttgtgac
    10381 cgttttactc acaattttca gccacctgtt acagtgaatt tagaaacaga attgttttct
    10441 cctcttgaag atattcttaa ttctcaatca gaaaatatga tagataatgt gatgcaattt
    10501 tatgccgagg attatctgta ttatggattg cagaaagagg atatccgaaa ttggttacag
    10561 agtatctata gtgttgcacc agaagcacaa gcacaggtca cacttataag ttctgaaatg
    10621 ctctctgaaa ctactgcttt agctgattgg agattgctta taaccacgga tagagagtca
    10681 actgtgttgg tggatagcct cttttattca gaacatattg cccagcgtga aggaaaatgg
    10741 ctcttaagag gaaatcaact ttctggctct tatccttcta caaaagagca tattataata
    10801 gaatatttca ccttcctcac ttgtcctaat tgtcccgtgg tagaaaataa gttaaataaa
    10861 tggcaggacg cttatccaca aagaataagt tatctggaac atcatattag cgaccaactg
    10921 gcaattccgg gagatgaggt ttatcaatat tatggttatc ctccttttcc cagttctata
    10981 attcaaggag aaatggctct tatgggaaac agtccagcca ttcttgcaga atatgatact
    11041 gccattcaga gtttactgga aaaagatgct gatattctgt atctttaccc ggttatccgg
    11101 ataaataaca aacaggttac aggttctatc actttacaac tgctaaatcc tgaactggat
    11161 ttaaccaatc tttacctgaa ttatgcgtta attgataagg tctctccctt tcaaaacacc
    11221 aattatgatt atgttcggaa tgtggtgctg gctcaaggaa gtaaaaccct tacttcttct
    11281 gatttagagg gaccaataag tttttcttta acctcatcca taactattcc aaatgatgcg
    11341 gcaatagtta ttttcgctca aactaaacct cctgtgtttg agaacaatgc caagattcat
    11401 agtggaatag agattgccct tgaccatatt attaaatcta aaaaataaaa agaggaacaa
    11461 aaaatgaaaa aattaacaat tctaactcta ctcttacttc tcgtagcaat attaatggct
    11521 gaaccaatgc cagatttccg tttgcctgat gaaagtggga agaatgtgag cttaagtgaa
    11581 cttttaggga agggacctat tatcattgat ttttgggcag attattgcca gccctgcaaa
    11641 caagctatgc cagctttaca agaactggtt aataagtatg atagcttgac ggtagtttta
    11701 atttctctgg atgcacccaa agctcaaccc aaagcaaaag cttatctgaa gagtaaaaac
    11761 tatagcttta ttacactatt tgatccagat aagactttag ccaagaaatt gaatgtggaa
    11821 aatccacctc atacttttat tctggataag accggagaaa tagtctattc tcatcttggt
    11881 tatgaaccag gggttgaaaa agagtatgag cataaggttc gtaaactttt aggtctggca
    11941 aatgaagaag gagaagaatg cacctgcacc gaaaaatgtc ccgattgtca gcaaaagtaa
    12001 ttttattacc acttattttt ctggcaggaa taacttttat ccctgctcaa actactttga
    12061 cggttaatgg tttaaatcaa accagtttcg tttatcgtgc tgcatccgat tctttaaatt
    12121 gctactttta cgattccttt gccttcaatc tgggctataa ggattttagt ttcgggatga
    12181 agtttattgc ggaactgcct aaatatagca ttgagcaaag tgaactttta gcagaactta
    12241 atccagaccg tttaaatctt ggctggaagg aactttatgc cagctataac cggaataact
    12301 atcaggttta tgctggaatt atggaagaaa cctttggcag tggattgatt tttcgcagtt
    12361 accaggattt ggaaatggat gatgactaca gagtgaatgg ttttcgcttt tcctatgata
    12421 ataaactgcg ggtgaaggca ctttattccg cttacagtaa tcctataaat agaggaaaat
    12481 tggatttagc ttatggagca gaccttcaat atcctatttt gaaacctttg actcttggag
    12541 ttactgccct ttctttacaa tcatatatag gctcttccta taaagaagat aatattctgg
    12601 gtggaagagt ttcatttcaa gaagattgga tagacggttc catagagctg gctcaacgcc
    12661 aaaaagcata tagtaatgat aatggctctg ccgtatatgg caatctcagc acttattttg
    12721 gtcctttaca gattggtggt gcttataaat actatgatag ctttgattac tttaaccatc
    12781 tgcaggatat ccctttagcc aatcatcata atgaaactct ggcagataat caaagctcgg
    12841 gcttggatga agaaggtttg cagggctgga tgagctttgc cttcttaaaa tactggactt
    12901 tcaatctgga ttatgcggaa gcctggaatc acaatcataa agtaaaaatg aatgatttct
    12961 ttgttagtct ggattggaat aaggatttaa aaatggcatc tatttcctac agtcaggtag
    13021 agaagataga tgaaattctc agtcactggc aaaaggaatc ctatcctggt ttcaatcttc
    13081 aatttcccgg aaagaaaaat ccttttaccc tatccggaga gtttaaagta gtggaaaagc
    13141 aggtttatga tgtggaaact tctcattatg aacctaaaat ccagtttgat accagtttta
    13201 ataaactttc ctgttctctg gcattacaga gttggtggaa agatttttcg cacctgacac
    13261 aaagtcgtta tatgccttct gtgcaagtaa aatatcctct tttttccgaa actgatatcc
    13321 tgttattggt gggaaaagaa gctggaggga aggtatgccg taatggaatc tgtcgttatg
    13381 tagctccatt ttctggcata aaattagaaa tgactacccg tttttaaata ggagattatt
    13441 atgaaaaaga cacttgttct catactcttg atgagtgtag cacttcttgc agctaatcca
    13501 aattattttc cgcgcacttc tttggcggaa ttatgtctga ttcaagacaa ttctgattgc
    13561 aatgaagcaa tgatgcaatt aaatgaggtg ctggataaca ccaatagagg agaatttatt
    13621 gctacccatc tttttcaaaa tgataataat ttaggaaatg tgagtgtgaa cgagaggata
    13681 aatgatttag gagtagagat tctgcccact atctttttta atcgggaaag ctctgtcaca
    13741 ggtgtcccag gaactgggat ttacaataat atagttaata gctataaatt ctccgcttct
    13801 cccttaaaaa tgaatattac cagctttaat actgaaacgg gtgctatttc agttacggtt
    13861 aaaatgcttg atcctgaact ttctttaatt aatcaaaagc tcatattgat tttagcgcag
    13921 aactctatgg gaacctataa taatgtggca aggcaatatc tctatcataa tataaattta
    13981 actggaccgg gcgaagaagt aacaatcaac gataattttt cctatgatgc ttctgtagag
    14041 cctgataata tctgggcaat agccttggta gaagttgata atcaggttat tttgcaaagc
    14101 acttccactc ttcctttgcc tgagtattat ctgcgttgtg cctatgattg ggagggaaat
    14161 aatatcattg ttgaccccaa ttccaatttt ctttcggaac cgttatggat attcaatctc
    14221 ggggcagcgg acacttttaa tatgagaata gtggtggatg atgctccagc caactggtat
    14281 ttcaattatt gcgatgaaga agggaattgc tatccgggtg gtatttctat gcccttaaac
    14341 ctgagttctg gacaggtgaa aggttttcat ctcaatctaa tggtcggttc tactggtact
    14401 gctcatttcc attttgaact cacttctcct aatcttggca cttataacat tccttttatc
    14461 tgtcacacca gtgaccctat acaggatgat attatccagg ttcctgctgt gatgactctt
    14521 aaagcaaact cacctaatcc ctttaaaaat atgacttcct tttctatatc tgcaaataaa
    14581 gcaggttatg ccactattca aatctttaat ctaaaaggag agctggtagc agaaactaat
    14641 tctcactcta tttatcaggg agagaataag atagagtgga aagtgccttc ccgtttggct
    14701 aatggaatct atttctacag acttaaggaa caacctgcat ctttagcaaa aatgctcttg
    14761 cttaaatagt aactttaaat caggctaact atatagggag aaaggaaata tgaaatttgt
    14821 ggatagattt cttacccgaa cggaacaaaa ggcaattttg gctatttgtg gtctggctct
    14881 attaggtttt atcctgggac aatttgatgg ttttcataat ctgttagcta aaaaaacggc
    14941 aacaccagag acaaccgaag aactggtgaa agctgtcaaa gaagataagc cagtacagat
    15001 tgatatccga accgcttcta aagaagaatt gattcttttg cccggaatag gtgaaaaacg
    15061 tgctcaggat ataattgact atcgtaacca atacggtttt aaaagcacag acgaccttct
    15121 tcagataaag ggtattggtg aaaagacctt agcaaaaatg ttgccctctt tattaatctt
    15181 tggaaatcca gaatttactc cagaaaaaat ccctgcttct ggacaggata cttctcaaat
    15241 agctatatct gataaaggta aaaatactga taaaacatca actaaccaga aagcaacgga
    15301 tgccaagaag aaaacctcct cagcttcagt tcctaaaagt cagctaacaa atatcgtaaa
    15361 tattaatact gcaggaatag aagaactttg taccctgccc ggtattggcg aagttaaagc
    15421 tcaagctata attgattatc gcaatcagaa cggtaaattt aatgcggtgg aggatatcct
    15481 taatgttaaa ggaattggac ctaaaactct ggaaaagatt cgctctcgca ttaaggtatg
    15541 actataattt aagcaataaa cagtttttct tctactgctc agaagtatat ctttaacctt
    15601 tcctgctggg tttgatatta ctctatcctt tatcttatga ttggaacatt attttgaaag
    15661 tgagtcagac aaatcctgac aggttagtcc tatttattgg aaagtaatat tttacctctc
    15721 taagacattc atatctaaaa ggggaattat taaaacactg cgggacctcc aactgaaaga
    15781 ggttaattga actaattcag tgcaaccata tatttaaata aatttatatt ctgtaatttt
    15841 atcacttgag tatataataa tcattcatca aatatttcaa tttacccgtt ttagcaatta
    15901 atttatgttc cccctttcgt tttatattgc ccatctctta cctttttctt agctccagta
    15961 atggggaaga gctaagagag agctaaggaa gaggggaaaa ttggataata gggaactatc
    16021 tgaatttata caattatgca gaaatttaaa caatatacat tataaagttg ttacttttat
    16081 ctcttctaac tatccagagc ttgaagaaaa ttaatcttca aaaataagta cagttggtta
    16141 taattatata atagatttaa ataaagcaag ataatctatg aactatagtc ctcccttatc
    16201 caaaaaaatt atactttccc cttccctcaa ttttattaag aggacaacaa taatactttc
    16261 aggcaaagag ctttttacta ttactgctga gttacagact ccctagggca gagaaaaatg
    16321 aagtaataga accaaaaata ataaaggtta atacaaagga tatagcaacg acaattaaat
    16381 aagctaatac ccaattcttt ctgttgggat tctcatcttt actggttaac caaactataa
    16441 tcat
//
LOCUS       DAORZF010000039        16433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_2870_length_16433_cov_352.044694, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000039 DAORZF010000000
VERSION     DAORZF010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 16433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16433
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2870_length_16433_cov_352.044694"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="PK015_05495"
     CDS             complement(<1..707)
                     /locus_tag="PK015_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33956.1"
                     /translation="MKKIILSVFVLVAIVGMVQAAPFQTMGLLRTPDAYVIPHKSAHL
                     TLVGYYRNVDEEEFIPYGMLGVGLFNRLQLDGFAGDDIYFMNAKVKLIPETLYIPQIS
                     VGMDNIFSTVNVQRAQDYNDPSEYSWADNPDKCDYEHYSPYVVASKQAVIGGISWMFN
                     LGGGANRFTGQASRARQLSGLFASVELSPAKNFSLQGEFDGQDFNVGLKYSLGNYSFK
                     VAAQAVENYAKNNGLENT"
     gene            794..1174
                     /locus_tag="PK015_05500"
     CDS             794..1174
                     /locus_tag="PK015_05500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOL33957.1"
                     /translation="MQNTETRNLFHTGEDLAVKYLIHKGFKILERNYRIQGGEIDIIA
                     QNEDTLIFVEVKTRKKHSMKMALMNISYTKQKRISLTAVRYINQHPENVKPKIRFDII
                     IVFYYYEDNTYSIKHLEDAFMPIV"
     gene            1513..1716
                     /locus_tag="PK015_05505"
     CDS             1513..1716
                     /locus_tag="PK015_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HOL33958.1"
                     /translation="MPRVTVDPFYCKGCGLCIIACPKKILRFSEDINDKGYHYAECFN
                     QEDCTACRLCYITCPDVAITIEK"
     gene            1725..2804
                     /locus_tag="PK015_05510"
     CDS             1725..2804
                     /locus_tag="PK015_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOL33959.1"
                     /translation="MSKILMKGNEAVAEAAIRAGCRLYFAYPITPQSELIEYMAKMMP
                     KVGGTFIQAESEIAAINMVYGAAGVGKRVMTSSSSPGISLKQEGISYMAGAELPAVIV
                     NVQRGGPGLGDIQPAQGDYFQAVKGGGHGDYQLIVLAPSSVQEFADLAADAFDLADKY
                     RNPVMILADGMLGQMMEPVEFKPAKTQEELDALANQHLSWCICPNADGDAKHHHEINS
                     LELDPEILEKKVSKLYEKYAEVEKNEVRYETYNLSDDNEILCVAWGTAARVVKSAINE
                     LVAQGKSIGLVRPITCWPYPYEAIASAIGPKVKKVYVFELNSGQMVEDVMIAVNGKVP
                     VDFWGKLGGIVFTPADIQAKLEACF"
     gene            2815..3564
                     /locus_tag="PK015_05515"
     CDS             2815..3564
                     /locus_tag="PK015_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOL33960.1"
                     /translation="MQVIAKRPETLTAEPFTYCPGCTHGIATRLIAEAIEEQGLINLM
                     AGVAPVGCAVFAYKFFNFDMAEAAHGRAPAVATGMKRGRPNMHIFTYQGDGDLAAIGT
                     AEIVHAANRGEHISVFFINNAIYGMTGGQMAPTTLAGMKTTTSPYGRNPEDIGYPIRM
                     CELLSSLVAPYYIERVSLLSPGDILRAKKAVNKAIQYNKEERGFTFVELISTCPTNWG
                     FDPDKAREWAKENMLPYYKLGVYRDKGEGVE"
     gene            3564..4118
                     /locus_tag="PK015_05520"
     CDS             3564..4118
                     /locus_tag="PK015_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOL33961.1"
                     /translation="MTTEMICAGFGGQGVLTIGRFLAQAGMKEGKNVSWLPSYGPEMR
                     GGTANVSTVISDGPIASPIVSYPDVLVALNQPSLDKFGPMVRKGGVIIYNTNMCPRGC
                     NRDDVQLIPVPMNDIANEIGSSIVLNMLAIGIIIGKTGLVKYETIEDELKSFLQEKNP
                     DLLDKNLQAIKKGIEIGKQYTSVK"
     gene            complement(4192..6618)
                     /locus_tag="PK015_05525"
     CDS             complement(4192..6618)
                     /locus_tag="PK015_05525"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOL33962.1"
                     /translation="MIPTLWYIAPIGAITALIFAAIFFHQVKKADPGNARMVEIASYV
                     REGAFAYLKQQYKVVALFFLLAFIVFNIMAQVLHVLHWLVPFAFLTGGFFSALAGFIG
                     MNMATLASNRTTQACSESLNKGLKIAFRGGAVLGLTVVGLALLDISLWFFILNLFPET
                     FSLRQITVIMLSFGMGASTQALFARLGGGIYTKAADVGADLVGKVEENIPEDDVRNPA
                     TIADNVGDNVGDVAGMGADLYESYAGSILATAALGMSAVTSLGENFAHLSINFVVAPM
                     ILAGIGILLSILGIFMVSTKEDAGPKELMFALNKGVYGSSILIAVAAFFVTKWLLPAD
                     YYFGVFTAILVGLTCGVLIGVFTEHSTSYSFKATRGIAEQANYGPATVILEGISVGMR
                     STAAPVITIVIGILLSFICSHGFQSIEMGLYGIGFGAVGMLATLGVTLAMDAYGPIAD
                     NAGGNAQMAELPEKVRVMTDTLDSVGNTTAATGKGFAIGSAALTAMTLLAAYFDEVRN
                     GLLLAGDKAGQHIFMYINYGKGLIDKVNVNDATVMDFINFYQIHVLNPKFLLGVFIGG
                     MVILFFAALTIKAVSNAAGKMVAEVRRQFREIPGILEGKAKPEYARCVTIATQGAQQQ
                     MLAPALVGILTPIIVGIVLGITGVLGVLTGAITCGFVMALMLNNSGGAWDNAKKYIEI
                     GNYGGKSSPAHKATVVGDTVGDPFKDTTGPSLNILIKLMSMVSIVFAGLIVNYSIQVE
                     KIQPPFSKQKVDISQQNQLIRIDKALYEQQQCLEKQSSQEQKNEPVPVIIDNNIGQRI
                     DSKNTEIKTP"
     gene            6979..8040
                     /gene="prfB"
                     /locus_tag="PK015_05530"
     CDS             6979..8040
                     /gene="prfB"
                     /locus_tag="PK015_05530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOL33963.1"
                     /translation="MRFGGYFDQEGKQKQINQLQEKMSQPDFWENPTEAKNISRKLSQ
                     LNEELEHIGTLDRLKGELETYLQLLEEDFSPALFEEAVQELPAQKQFIEKAEIECYLN
                     DKYDYEDAILVIHSGAGGTEAQDWAEMLLRMYTRWAEENGFQYNIIDLLPGEEAGIKS
                     VSMEIKGPYAYGMLKAEIGIHRLVRISPFNAQGKRQTSFASVYVYPEFEEDIEVDIDP
                     KDLRIDTYRSSGAGGQYVNTTDSAVRITHIPTGIVVSCQNERSQIQNREKAMAILKSR
                     LYQYYEEQREAEKQKEESAKSDIGWGHQIRSYVFQPYQMVKDHRTGYEVGTVDKVMDG
                     DLDNFIYAWLKQKALEKAQ"
     gene            8048..8791
                     /locus_tag="PK015_05535"
     CDS             8048..8791
                     /locus_tag="PK015_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOL33964.1"
                     /translation="MNYKKLLPEVDLQNLPNHIGIIMDGNGRWAAKRHRPRVYGHRAG
                     ARAVRKVVELATEIKLGYLTIYAFSTENWKRPANEVQALLKLLKDYLIKELPELNREN
                     IKVRIIGTQDRLDPSYLTEIHQLAGETAQNTGLNLNIAFNYGGRLEIIEGIKKLYQLA
                     QNNPELVDNLTPQTFGNYLYTAGQPDPDLIIRTSGEYRLSNFLLWQSAYAEIYITETL
                     WPDFDKVEFLKAIIDFQGRKRRFGGIESE"
     gene            8788..9576
                     /locus_tag="PK015_05540"
     CDS             8788..9576
                     /locus_tag="PK015_05540"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOL33965.1"
                     /translation="MTELQKRVLVAVIFIPILLAVLYLGGAYLAVTFALIVILGSAEY
                     IAMLRKASFSVSWYWLAIIFAVFVIMSFLTGWELIIGWIIFFLAVLEALLKWNEQKSI
                     PQIALELFGLFYIGVIPAMCLRLGLNFRHPNLLLWLVISIWVTDSVAYFIGMRWGRHR
                     GIFSVSPKKSTEGFIAGIIAPFIIMIILYNIGIIKSHQDLILVAVAAGIFGQLGDLVE
                     SMIKRFCGVKDSSKLIPGHGGILDRFDSILLAGSFLYCSLIILQ"
     gene            9591..11321
                     /locus_tag="PK015_05545"
     CDS             9591..11321
                     /locus_tag="PK015_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HOL33966.1"
                     /translation="MIKKILLPVLMLGFVSLIFAQAVDLFISEYVEGSSYNKAIEIFN
                     GTGAPVDLSQYTVKLASNGGAWSTTNSVTLSGTLNNNDVFVIANTQANAAIQAVADLL
                     HNVTNFNGNDCIGLFHGTTLIDIIGVYQQDPGTAWPVAGIDGATLNHTLIRKPEVIQG
                     NTDWIASAGTNLDNSEWIVYPQDYIDNLGSHTFNPGGSQHCATPTFNPPGGVYTGPIS
                     VTISSTTPGATIHYTLDGTDPTESSPVYNTPISLHTNTTLKARAYASGYAPSYIATAN
                     YFFPIVVSNLTELRAQTAGDGTVYMVSGKVVLTFQQNFRHQKYVQDYQAGILIDDPSG
                     VITTTYNLLDGITGLTGTIAYYNNMLQLTPVIDPGPATSSNNDINVPTVTIAQINANV
                     ATYQARLVRIRNAHFSETGIFATGQNYTLIDDTGSIVFRTTFYDVDYIGQPIPTGNFN
                     IWVLVNQFNQTPQVTARMLSDWSDVPVNDAIETPQVAQLIGNYPNPFNPSTTISFSTT
                     KNEPVQITIFNQKGQIVRTWDIDSKEDGIQHLVWDGRDQNGNPVTSGVYYYRMKSGKY
                     SSSRKMVLMK"
     assembly_gap    11537..11636
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11797..13932)
                     /locus_tag="PK015_05550"
     CDS             complement(11797..13932)
                     /locus_tag="PK015_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HOL33967.1"
                     /translation="MTERKVLAEFPYELKPVEKGYNRRTLYINLSTKAIESRPVTDLM
                     IDKFVGGKGFDLYLMWHSVKDTTRWNDPENEICIAFGPLCGNTNYPGSGKSIVTTISP
                     LTDIPIDCNVGGHFGPYAKFSGWDAIEIQGKSEEEIIVFIDGDKGIVQILSAPDEEIN
                     SHILAERLIAEFADNPNGYQYVSVVSSGKAAEYVPISCLNFSFWDKRRKVARLKQAGR
                     GGTGMVFRDKKIKALVVKYSGLKGDTNHPVDLETLQKTGLKLHKEIEEGDDKQNRMRQ
                     VGTAHLVEIMNDYDLLPIKNFKYGQMEEAKGLASWEFKKLFTQGLPDGCWFGCSLSCC
                     KAVDNFIPRTGPYKGQKVTVDGPEYETVGGCGSNLCIWNARDVVELNFYCDTYGIDTI
                     SFGTSCAFAMECYEYGILNKERTGGLDLSWGNADSVFELIHQMGRGEGFGVIVGQGVH
                     KMKEIFAREYGADPKLLQDIGMEAKGLEYSQYMSKESLAQQGGYTLALKGPQHDEAWL
                     IFMDMVNNQIPTFEDKAEALHYFPMFRTWFGLQGLCKLPWNDIEPANNAETDEPAKVP
                     EHVQNYIDIYTAITGRPLDKEELIRQSERVYNFQKVFCMRMGKGRRIDDIPPYRAVGP
                     VTEEEYLSRQERYDKLLKEKQGIDPEGKSTQEKMALLRAYREDQYQQLVDAVYKRKGW
                     TKEGIPTIEHLKSLGMDLPEVVEVVKRFL"
     gene            complement(13935..14330)
                     /locus_tag="PK015_05555"
     CDS             complement(13935..14330)
                     /locus_tag="PK015_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOL33968.1"
                     /translation="MKKFLNTYDEDCIACHACEISCARIYFKVDDLERSRIRIEERDK
                     SAHIVACNQCETCVQVCPTLALTVNAQGVVLVNNRLCVGCYMCIAACPNESMFRYLGG
                     IIPIKCIACGLCLDSCPTEAIRIEEKQEN"
     gene            complement(14389..16290)
                     /locus_tag="PK015_05560"
     CDS             complement(14389..16290)
                     /locus_tag="PK015_05560"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOL33969.1"
                     /translation="MTEVELISRLQEQAIIARSAILTMTTLAGSGHPGGSMSSIDLLL
                     TLYQFIRHNPQNPELPDRDRVVVSNGHISPAVYTALALNGYHNLDEVISQFRLSGSIY
                     EGHIERDVKGVEWSTGNLGQGLSAGAGFALASRINNIPYNVYVLMGDGEQQKGQISEA
                     RRFAIKYQLNNLTAIVDYNELQISGNIHSVMPQNIKANWESDGWKVIEIDGHNYQEIL
                     STLQMTRDKDYPLMILAHTVMGKGVPFMENQAKYHGAALNEAQLEEALHYLGQSNRLE
                     DYKMMRAEFKPVKQKPTKDKYALQCNLKSGNPMLYEKPTDNRSAWGNAIADLAEINKN
                     NSTPIVVTDCDLQSSVKTAAFEKVTPDRFFQGGIMEHNTAVLSGALSTCGIQSFWSDF
                     GVFGLDEVYNQQRLNDINHTNLKVVLTHTGLDVGEDGKTHQCIDYIGIVRNWYGFHLI
                     IPADPNQTDRLIRWLIDKPGNYVVTMGRSAVPIILKDDGTPFYDLNYAFDYGKCDILR
                     KGDKATVMVTGTPIGNAIKAVDTLKEEGINLQLIYVSCPLAINRDTLIQAVNTGLIFS
                     IEDHNIHSGLGSIIADRLAEENLSARLIKIGVKNYGSSGTSNDLYKVVGLDAESIKER
                     IKQELINKD"
     gene            complement(16357..16432)
                     /locus_tag="PK015_05565"
     tRNA            complement(16357..16432)
                     /locus_tag="PK015_05565"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16397..16399),aa:Ala,seq:ggc)
BASE COUNT         5086 a         3126 c         3013 g         5108 t
ORIGIN      
        1 gtattttcca gtccattatt tttagcataa ttttccactg cctgagctgc tactttaaag
       61 ctgtaatttc ccagcgaata ttttaaacca acattaaaat cttgaccatc aaactcaccc
      121 tgcaatgaaa aattcttggc aggcgataat tcgacagaag caaataatcc acttaattgt
      181 ctggcacgac ttgcttgtcc ggtaaaacga ttagctccac cgcccagatt aaacatccag
      241 gagatgcctc caattaccgc ttgtttggat gctacgacat aaggtgaata atgctcataa
      301 tcacatttat ctggattatc tgcccaggaa tattccgaag gatcattata atcctgagct
      361 ctttggacat ttacggtgga aaagatatta tccataccta cggaaatctg tggaatatat
      421 aaggtttcag gtatcaattt aaccttagca ttcataaaat aaatatcatc tcccgcaaag
      481 ccatccaatt gtaaacgatt aaagagacct actcctaaca ttccataagg tatgaattct
      541 tcctcatcca cattacgata atagcctact aatgttaaat gagcagactt gtgaggaata
      601 acatacgcat caggagtgcg caacaatccc atagtttgaa atggtgctgc ttggaccatc
      661 ccaactatcg caacaagaac aaatacactt agtattattt ttttcattct tccttccctc
      721 cacctttcgg tgatttttta atctcatgtt caaaatatca ttgacttatt attcgtcaag
      781 ttatttttga tttatgcaaa atacagaaac cagaaatctt ttccatacgg gtgaagacct
      841 tgccgttaaa tatctaatcc acaaaggatt taagatcctc gaaagaaatt atagaatcca
      901 gggcggtgaa atagacatta ttgctcagaa tgaagataca ctaattttcg ttgaagtcaa
      961 aacccgtaaa aaacactcta tgaaaatggc tttaatgaat atctcttata caaaacaaaa
     1021 gagaatatca ttaactgctg taagatacat taatcagcat cctgaaaatg tcaagccaaa
     1081 aattcgtttt gatataataa ttgtatttta ttattatgaa gacaatacct atagtattaa
     1141 gcatcttgaa gatgcgttta tgccaattgt ctgaaattct gggataataa aattacttat
     1201 ttcatctaat aaaatgccag ttggtctatt aataattaaa taatgtagtt cagcttataa
     1261 ccaagtttag tcatattctg gacataagtt agtcataagt aggacataag ctggtcataa
     1321 ttaaatatct ataagtgaag aaaaaataat tttaccagaa atgattccta atgtcctaat
     1381 atttatttag ttatgagtgt aagttgcata tctattcttt aacctttgtt ttgaagaaat
     1441 aatttgacag aaagagctgt ttataagctt tatcacaata ttaaaatatc aataaaataa
     1501 aggagtaaag ccatgccgag agtaacggta gacccgtttt attgcaaagg ttgtgggctc
     1561 tgtattattg cctgtccgaa aaaaattcta agattttctg aggatatcaa tgataagggt
     1621 tatcactatg ctgaatgttt taatcaggaa gattgtaccg cctgtagatt gtgttatata
     1681 acttgtccgg atgtggcgat aacaattgaa aagtgaggtg aatgatgtct aaaatattaa
     1741 tgaaaggaaa tgaagcagta gctgaagcag ctataagagc tggttgcaga ttatattttg
     1801 cctatcccat cacgcctcag agcgaattga tagagtatat ggcaaagatg atgcctaaag
     1861 taggtggaac tttcattcaa gccgaaagtg aaattgctgc tataaatatg gtctatggtg
     1921 cagcaggggt agggaagaga gtgatgactt cctcttcttc tcctggcata tctttaaagc
     1981 aagaaggtat ttcttatatg gctggagcag aacttcctgc ggtgattgtt aatgttcaaa
     2041 gaggtggtcc tggacttggt gatattcaac ctgcccaggg tgattatttc caagctgtta
     2101 aaggcggggg acatggtgac tatcaattaa ttgttcttgc tcccagttct gttcaagaat
     2161 ttgctgatct ggcagctgat gcttttgatt tagccgataa atatcgtaat cctgtgatga
     2221 ttttagctga tggtatgctc ggacagatga tggaacccgt agaatttaaa ccagcaaaaa
     2281 ctcaggaaga attagatgct ttagcaaatc aacatttatc ctggtgtatc tgtcccaatg
     2341 cggatggaga tgcaaagcac catcatgaga taaattccct ggagcttgac cctgaaattt
     2401 tagaaaaaaa ggtaagcaag ctttatgaaa aatatgcaga agtagaaaaa aacgaagttc
     2461 ggtatgaaac ctataatcta tccgatgata atgaaatcct atgtgttgcc tggggaacag
     2521 ctgcacgtgt ggttaaatct gcaattaatg agcttgtagc tcagggtaaa agtatcgggt
     2581 tagtgcgtcc tataacctgc tggccctatc cttatgaagc tattgcctct gctataggtc
     2641 ctaaagtgaa gaaagtatat gtatttgagt tgaattcagg acagatggta gaagatgtaa
     2701 tgattgcagt gaatggcaaa gttccagtag atttctgggg taaattaggt ggtatagtct
     2761 ttactccagc agacattcaa gccaaacttg aagcttgttt ttaaggagga aatgatgcaa
     2821 gttatagcaa aaagacctga aaccttgacc gctgaaccct tcacttattg tccaggttgc
     2881 actcatggta tagcaactcg tcttattgca gaagcaatag aagagcaggg tttaattaat
     2941 cttatggctg gagttgcacc agtcggatgt gctgtttttg cctataaatt cttcaatttt
     3001 gatatggcag aagctgctca tggtcgggct ccagcagttg caacaggtat gaaacgtgga
     3061 agacctaata tgcatatttt cacctatcaa ggtgatggtg atttagctgc tattggaact
     3121 gcagagattg tgcacgcagc taatcgtggt gaacatatca gtgtcttctt tattaataat
     3181 gctatctatg gaatgacggg tggtcaaatg gcacctacaa ccctggctgg aatgaaaact
     3241 actaccagtc cttatggaag aaatcctgaa gatataggct atcctatcag aatgtgtgaa
     3301 cttctgtctt ctctggttgc tccttattat attgaaagag tttcacttct tagtcctgga
     3361 gatatccttc gtgctaaaaa ggcagttaat aaagctattc aatataataa agaagaaaga
     3421 ggtttcacct ttgttgaact tataagcacc tgcccaacga actggggttt tgatccagat
     3481 aaagccagag aatgggcaaa agaaaatatg ttgccctatt ataaacttgg tgtatatcgt
     3541 gataaaggtg agggggtaga ataatgacta cagaaatgat ttgtgctgga tttggtggac
     3601 agggggttct tactattggt agatttcttg ctcaagcagg aatgaaagaa gggaagaatg
     3661 tttcctggct accctcctat ggtcctgaaa tgcgtggtgg cactgctaat gtttccaccg
     3721 ttatttcaga tggacccatt gcatctccca tagttagtta tcctgatgtg ttagtggctc
     3781 ttaatcagcc ttcattggat aagttcggtc ctatggttcg taaaggtgga gtcattatct
     3841 ataacacaaa tatgtgtcct cgtggttgta acagagatga tgtacaattg attcccgttc
     3901 caatgaacga tattgctaat gaaattggct catctattgt attaaatatg ctggcaatag
     3961 gcattattat tggcaaaact ggtctggtta agtatgaaac tattgaagat gaactgaaat
     4021 cgttcttgca agaaaagaat cccgatttat tagataagaa tctacaagct attaaaaaag
     4081 ggatagaaat agggaaacaa tatacctcag taaaataaat taagcataaa aaacaggtgc
     4141 gagaaatatt cctttcgcac ctgttttttt tatgagataa ctttcttttg attaaggagt
     4201 ttttatttct gtgttctttg aatctatcct ttgtccaata ttattatcta tgatgacagg
     4261 aacaggttca tttttttgtt cctgtgaact ttgcttttct aagcactgtt gctgttcata
     4321 aagtgcttta tcaattctga ttagctggtt ttgttgtgat atatccactt tttgtttact
     4381 gaagggtggt tgaatctttt ctacctggat ggaataatta acaatcagtc ctgcaaatac
     4441 gatggagacc atactcataa gttttataag aatatttaaa gagggacctg ttgtatcttt
     4501 gaacgggtct ccaaccgtat cacccactac agtagcttta tgagcaggag aagatttacc
     4561 tccataattt cctatttcaa tatacttttt tgcattatcc catgcaccac ctgaattatt
     4621 tagcattaaa gccataacaa agccacaggt tatagctcca gtaagcaccc ctaaaactcc
     4681 ggttatacca agcactatac caactataat gggagtaaga atccctacta aagctggggc
     4741 taacatctgt tgttgtgctc cttgagtggc aatagttacg catctggcat attctggttt
     4801 tgcttttcct tccagaatac caggaatctc tctgaattgt ctgcgcactt ctgccaccat
     4861 tttacctgct gcattactta cggctttaat agtcagtgca gcaaagaata atattaccat
     4921 ccctccgatg aagactccta ataagaattt tggattaaga acatgaattt ggtagaagtt
     4981 tataaaatcc attacggtag catcgtttac atttacttta tcaatcagtc ccttaccata
     5041 atttatatac ataaagatat gctgtccagc tttatctcct gcaagcagta aaccatttcg
     5101 cacctcatca aaataggcag ccagtaaagt cattgccgtt aaagcagctg aacctatggc
     5161 aaatcctttt cccgtggcag cagtagtatt tccaacacta tccagagtat cagtcataac
     5221 ccgaactttt tccggtaatt cagccatctg agcattacca ccagcattat ccgcaatagg
     5281 accgtaagca tccattgcca aagtcacacc aagagttgcc agcattccaa ctgcaccaaa
     5341 accaatacca tatagaccca tttcaatgga ttgaaaacca tgactgcaaa taaaagataa
     5401 aaggataccg ataacaattg taataacagg tgctgccgta gacctcattc ctacagatat
     5461 tccttctaag attactgtag caggtccata gtttgcttgt tctgctattc ctctcgtagc
     5521 cttgaaacta tatgaagttg aatgttcggt aaagacacca attaagacac cacaagtaag
     5581 acctactaaa atagcagtaa acacaccgaa ataataatct gcaggaagta gccattttgt
     5641 tacaaagaaa gccgctacag ctatgagaat agagcttcca taaacacctt tatttaatgc
     5701 aaacatcagt tctttaggac cggcatcttc cttagtacta accataaata ttccaagaat
     5761 ggaaagaaga atacctattc ctgccaatat cattggtgca accacgaaat tgatgcttaa
     5821 gtgagcaaaa ttttcaccta aagatgttac ggcactcatt cctaaagcag cagtggctaa
     5881 tatagagcct gcatagcttt cataaaggtc tgctcccatt cctgccacat cacctacatt
     5941 atcacctaca ttatctgcaa tagttgcagg attacgaaca tcatcttccg gtatattttc
     6001 ttcaactttt ccaaccaggt ctgctccaac atctgcagct ttagtgtaaa ttccacctcc
     6061 aagacgagca aataaagctt gagtggatgc acccatacca aaactgagca ttattactgt
     6121 aatctgtctt aaggagaaag tttccggaaa taagttcaaa ataaaaaacc ataatgaaat
     6181 atccaggagc gcaagaccaa ccacggttaa tcctaaaaca gcacctcccc gaaaggctat
     6241 ttttagtcct ttattcaaac tttctgaaca ggcttgagtg gtgcgattag aagccagagt
     6301 tgccatattc attcctatga aaccggctaa agcactaaaa aaacctccag tcaaaaaggc
     6361 aaatggaact aaccaatgta gaacatgtag cacctgtgcc attatattaa agactataaa
     6421 tgccagcagg aagaatagtg ccactacttt gtattgttgt tttaaataag caaaggcacc
     6481 ttctctcaca tagcttgcga tttctaccat acgagcattt cctggatctg cttttttcac
     6541 ttgatggaaa aatattgcag caaagattag agctgtgatt gctccaattg gtgcaatgta
     6601 ccagagtgtt ggaatcatat tcatcacctc tattcagtaa gtatgtaata gaaacttaaa
     6661 tcttttgctt ttctataata acctgctcct attgtcaata tttttctgtt taaaacttaa
     6721 tgaagcagcg ataatatatt tcctatctca ttaatttact gtatattatg gaaatcggag
     6781 aatctaatgc ttagagttcg ttattatatc atctttaact attgaaatgg ataacattga
     6841 tatgagataa agaataattt atttgacaat accagtgttt taagattaaa ggattaaaag
     6901 aatggaaaaa atgaggataa aataatatgg aatatataga ttacaaaaaa gaagttaact
     6961 caatgctttc ccagatttct gagattcgga ggttactttg atcaggaagg gaagcaaaag
     7021 cagataaatc aattacaaga aaagatgagt cagccagatt tctgggaaaa tccgacggaa
     7081 gccaagaata taagccgaaa acttagccag ttaaatgaag aattagagca tattggaact
     7141 cttgaccgct taaaagggga acttgagacc tatcttcagt tattagaaga agatttttct
     7201 cctgctctat ttgaagaagc agtgcaagag ttgccagctc aaaagcagtt tatagaaaaa
     7261 gctgaaattg aatgctatct gaacgataaa tatgattatg aagatgccat tctggtaatt
     7321 cattctggtg cagggggcac agaagctcaa gattgggcag aaatgttgct tagaatgtat
     7381 acgcgttggg cagaagagaa tggttttcaa tataatatta tagaccttct tccgggtgaa
     7441 gaagcgggaa ttaaaagtgt cagtatggaa attaagggtc cctatgctta tggaatgctg
     7501 aaagccgaaa ttggaattca tcgtttagtt cgtatctcac cttttaatgc tcaaggaaaa
     7561 agacagactt cttttgcttc agtatatgta tatcccgaat ttgaagaaga tattgaagtt
     7621 gatattgatc ctaaagacct tagaattgat acctatcgtt ccagtggtgc aggaggacaa
     7681 tatgttaata ccactgattc tgctgtaaga atcactcata tacctacagg cattgtggtt
     7741 tcttgtcaga atgagcgttc tcagattcaa aatagagaga aggctatggc aatcctgaaa
     7801 tccagacttt atcaatacta tgaagagcaa agagaagctg aaaaacaaaa ggaagaatct
     7861 gctaaaagtg atattggctg gggacatcaa attcgctcct atgtgtttca accttatcaa
     7921 atggttaaag accatcgcac aggttatgaa gtagggacag tggataaagt tatggatggt
     7981 gatctggata attttattta tgcttggctg aaacaaaaag cattagaaaa agcacaatga
     8041 gggaagattg aactataaga agctattacc cgaagttgat ttacaaaatc tgccaaatca
     8101 tattggaatc attatggatg gaaatggacg ctgggcagca aaaagacacc gtccacgcgt
     8161 ttatggacat agagctggag ctcgtgcagt tcgcaaagta gtagagcttg ccacggaaat
     8221 taaactcggt tatctaacta tttatgcttt ctccacggaa aactggaaga gaccagcaaa
     8281 tgaagttcaa gcattattaa aactattaaa agattatcta attaaggaac ttccagaact
     8341 gaatagagaa aatattaaag ttagaattat aggaactcag gaccgattag acccttctta
     8401 tttaacagaa atccatcaac ttgcaggaga aacagcgcag aatacaggtt taaatcttaa
     8461 tattgccttt aattatggcg gtagattgga aattatagag ggaatcaaga aactctatca
     8521 attggcacaa aataatccgg aattggtaga taatttgact cctcaaacct ttggaaatta
     8581 cctttatact gcaggtcaac ctgatccaga tttaattatt cgcaccagtg gtgaatatcg
     8641 tttatccaat tttttgctct ggcaatctgc ttatgcggaa atttacatca cagaaacact
     8701 ctggcccgat tttgataaag ttgaatttct caaagcaata attgattttc aaggacggaa
     8761 acgtcgtttt ggaggtattg aatcagaatg acagaattgc aaaaaagagt tctggtcgca
     8821 gtaatattta ttcccattct tctggcagtg ctgtatctgg gtggagctta tttagctgtt
     8881 acttttgccc taattgtgat tcttggttct gccgaataca ttgcaatgtt gcggaaagcc
     8941 tctttttctg tttcctggta ctggttagct attatttttg ccgtgtttgt cattatgtct
     9001 tttcttactg gatgggaact aattattggg tggataatat tttttctggc agtcctggaa
     9061 gcattgttaa aatggaatga acaaaaatct attcctcaaa tagctttaga gttatttggt
     9121 ttattttata taggtgtaat tcctgctatg tgtcttcgtc tgggtttgaa ttttagacat
     9181 cccaatctgc ttttatggtt agtaatttca atctgggtaa ctgattctgt agcctatttt
     9241 ataggaatga ggtggggaag acatcggggg attttctctg ttagtcctaa aaaatctact
     9301 gagggtttta ttgcaggaat tattgctcct tttattatta tgattatatt atataatata
     9361 ggaattatca agagtcatca agacttgatt ttagttgctg ttgctgctgg catatttgga
     9421 caattaggag acctggtgga aagtatgata aaacgctttt gcggtgtaaa agatagctct
     9481 aaattaattc ctggacacgg aggtatactg gatagatttg acagtatcct tctggctggt
     9541 tcttttttat attgttcttt aataatacta caataagtga ggtgaaaaaa atgattaaaa
     9601 agattctatt acctgtttta atgctgggct ttgtatcgct gatatttgct caagcagtag
     9661 acctgtttat ctcagaatat gttgaaggta gctcctataa caaagctatt gaaatattca
     9721 atggaactgg agctcccgta gacctttctc agtatacggt taaactggct tccaatggag
     9781 gtgcctggtc tacaactaat agtgttaccc tctctggaac tttaaacaat aatgatgtgt
     9841 ttgtcattgc taatactcaa gcaaatgcag ctattcaagc agtggcagat ttacttcata
     9901 atgttactaa tttcaatggg aacgattgca taggtctatt ccatggaaca accttaattg
     9961 atatcatcgg tgtatatcaa caagaccctg gaaccgcttg gcctgtagct ggaattgatg
    10021 gtgctacttt aaatcatacc ttaattcgta aaccggaagt tattcaaggt aacacagatt
    10081 ggattgccag tgcgggtaca aatctggata attcagaatg gattgtctat cctcaagatt
    10141 atattgataa tctgggttct cataccttca atcctggcgg ttctcaacat tgtgccactc
    10201 caacttttaa tcctccaggg ggtgtctaca ctgggcctat ttctgtaacg ataagttcaa
    10261 ctactccagg tgctactatc cattatactt tagatgggac agatcccact gaatcttctc
    10321 cagtatataa cactcctata tctttacata ctaataccac tctcaaagca agagcttatg
    10381 ccagtggcta tgctcccagc tatattgcta ccgcaaacta tttctttccc atagtagtta
    10441 gtaatctaac tgaattgaga gctcaaactg caggagatgg aaccgtatat atggtatcag
    10501 gaaaggtggt tttaactttc cagcaaaact ttagacatca aaaatatgtt caagattatc
    10561 aggctggaat tctgattgac gatccttcag gtgttataac taccacctat aatctgctgg
    10621 atggaatcac aggtctaacg gggactattg cctattataa taatatgcta caattgacac
    10681 cggtaataga tccaggtcct gccacttctt ctaacaatga cattaatgtg cctaccgtta
    10741 ctatagctca aattaatgcc aatgttgcca cttatcaagc tcgcttagtg cgcatcagaa
    10801 acgctcattt ctccgagacc ggaatctttg ctacgggaca aaactatact ttaattgacg
    10861 atacgggctc aatagtcttc cgcactactt tctatgatgt agattatatt ggacagccca
    10921 tcccgacagg aaatttcaat atctgggtgt tggtaaatca attcaatcaa actccacagg
    10981 taactgctcg gatgttatcc gattggagtg atgttcctgt taacgatgct atagagacac
    11041 cacaagtggc tcagttaatt ggaaattatc ctaatccttt caatcccagt accaccattt
    11101 ctttctctac aactaaaaat gaacctgttc aaataactat ctttaaccag aaaggtcaga
    11161 tagtgagaac ttgggatatt gattctaaag aagatggtat tcagcatctg gtttgggatg
    11221 gaagggatca aaatggtaat cccgttacca gtggtgttta ttactatcgt atgaaatcag
    11281 gtaaatattc atccagccgt aaaatggttt taatgaaata aacttaaatc aaaagctgtt
    11341 tcacttaaaa acagcttaaa ataaaaggtc ttgcccatat atctttaggt ataaaaagca
    11401 agaccttttt tatatttact ttattatgaa aagcttataa cgaaccatat catatagatg
    11461 cttatattac tttaaaatca gcaaaagttt ctctatcctt acgctttcct gcctctcact
    11521 tatgaaaaga gctaagnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnagag
    11641 ctaagaaaga gctaagggag aaaagggaat atactgaagg agaatacttt aacagtaatt
    11701 ataaaaatat tcctctatgc ttttctcata atgatactta cactttcagg atttattttc
    11761 cttacttaga gtaatttctg aaagaacgat ggaattttag agaaagcgtt taactacttc
    11821 tactacttca ggaaggtcca ttcccagact tttcagatgt tcaatcgtgg gaattccttc
    11881 tttagtccat ccctttcttt tatagactgc atctaccagt tgttgatact ggtcttctct
    11941 ataagcacga aggagagcca ttttttcttg agtagactta ccttcggggt ctattccttg
    12001 tttttccttt aacagtttat catagcgttc ttgacgagaa agatattctt cctctgtgac
    12061 aggaccaact gctctataag gtggaatatc atcaatgcga cgacctttac ccattctcat
    12121 acagaaaacc ttttgaaaat tataaactct ttccgattgg cgaataagtt cttccttatc
    12181 cagtggtctt cctgtgatag cagtatagat atcaatataa ttttgcacat gctcaggaac
    12241 ttttgctggt tcatcagttt ccgcattatt ggcaggttct atatcattcc aggggagttt
    12301 acatagaccc tgtaagccaa accaggtgcg gaacatagga aaatagtgaa gagcttcggc
    12361 tttatcttca aaagtgggaa tctgattatt gaccatatcc ataaagatta accaggcttc
    12421 atcatgctga ggtcctttca gggctaaggt atagccacct tgttgagcta aagattcttt
    12481 ggacatatat tgagaatatt ccagaccttt agcttccatt cctatatcct gtaatagttt
    12541 tgggtcggcg ccatattcac gcgcaaaaat ctctttcatt ttatgtacac cctgtccaac
    12601 tatcactcca aaaccttcac ctcgtcccat ttgatgaatc aattcaaaaa cactgtcagc
    12661 gtttccccaa cttagatcaa gacctccagt gcgttcttta tttagtattc catattcata
    12721 gcattccata gcaaaagcac aactcgtacc aaaggaaatg gtatcaatgc cataggtatc
    12781 acaatagaaa ttgagttcca ccacatctct ggcattccag atacaaagat ttgagccaca
    12841 acctccaacg gtttcatatt caggtccatc aacggtcact ttttgtcctt tataaggtcc
    12901 ggttctgggg ataaaattat ccactgcttt acaacaagaa agagaacagc caaaccagca
    12961 tccatcaggt aatccctgcg tgaaaagttt tttaaattcc caggaagcca accctttagc
    13021 ttcttccatt tgtccatatt tgaagttctt gattgggaga aggtcgtaat cattcattat
    13081 ttcaaccaga tgagcagttc ccacttgacg cattctattc tgtttatcat cgccttcttc
    13141 tatttcttta tggagtttga gtccggtttt ctgcaaagtc tccaggtcta cgggatgatt
    13201 agtgtcacct tttaatccgg aatatttaac caccagcgct ttaatctttt tatcccgaaa
    13261 aaccattcct gtcccacctc tacctgcctg ttttaacctt gccacctttc ttcttttgtc
    13321 ccagaaagaa aaatttaaac aagagatggg aacatattct gctgcctttc cgctggaaac
    13381 cacggaaaca tattgatagc cattgggatt atctgcaaat tcagcaataa gtctttctgc
    13441 caggatatgg ctatttatct cttcatctgg tgctgaaagt atttgcacta tccctttatc
    13501 accatctata aagacaatga tttcttcctc ggatttgcct tgaatttcta tggcatccca
    13561 gccagaaaac ttggcataag gtccaaaatg tcctcccaca ttgcaatcta taggaatatc
    13621 cgtaagaggt gaaatagttg tgacaatgga ttttccactt cctggataat tggtgtttcc
    13681 acaaagagga ccaaatgcaa tgcaaatttc gttctcaggg tcattccatc tggtggtatc
    13741 tttaactgaa tgccacatta aatagaggtc aaatcctttt ccacctacaa atttatctat
    13801 cattaaatct gttacaggac gggattctat tgctttcgtg ctaagattga tatataaagt
    13861 cctgcgatta taaccttttt ctacaggttt tagttcataa ggaaattcag ccaacacttt
    13921 gcgttctgtc attattaatt ctcctgtttt tcttctattc tgattgcttc agtaggacaa
    13981 ctatccaggc ataatccaca ggcaatgcat ttgatgggaa ttatgccacc aagataacga
    14041 aacattgatt cattaggaca agcagcaata cacatataac aacctacaca aagacgatta
    14101 ttaactaaaa ctacaccctg ggcattaact gttaaagcca gagttggaca aacctgaacg
    14161 caggtctcac attgattgca ggctactata tgagcagatt tatctcgctc ttcaatacgg
    14221 atgcgagaac gctccaggtc atctacttta aaataaatac gcgcacagct gatttcacaa
    14281 gcatgacagg caatacaatc ttcatcgtaa gtattcagga actttttcat cccttgctcc
    14341 taaataatat cttttataaa atctttttgt ttaaataagg actaattgtc aatctttatt
    14401 tatcagttct tgttttattc tctcttttat ggattcagca tccagaccaa ctactttata
    14461 taaatcgtta gaagtgccac tgcttccgta gttcttaact ccgattttga ttaagcgagc
    14521 agaaagattt tcttcagcta aacgatctgc aattatagag cctaaaccgc tatggatatt
    14581 gtgatcttct atgctgaaga tgagaccagt atttacagct tgaattaagg tatcacggtt
    14641 tattgccaga ggacaagaaa catatattaa ctgtagattg attccttctt ctttcaaagt
    14701 atccactgct tttattgcat ttccaatagg ggttccagta accatcacag ttgctttatc
    14761 accttttcgc aaaatatcgc atttcccata gtcaaaagcg taattcaaat cataaaaagg
    14821 tgtgccatca tctttcagaa ttatcggaac agcagacctt cccatcgtta ccacatagtt
    14881 tccaggtttg tctattaacc agcggataag gcggtcagtt tgattaggat cagccggaat
    14941 aatcaaatga aaaccatacc agttacgaac aattcctatg taatcaatgc attgatgagt
    15001 ttttccatct tctcccacat ccagaccagt atgagttaaa accactttaa gattggtatg
    15061 attgatatcg tttaatcttt gctgattgta aacctcatcc agtccaaaaa ctccaaaatc
    15121 agaccagaag gactggattc cgcaagtaga aagagctcca gaaagaacgg cagtattatg
    15181 ttccattatt ccaccttgaa agaaacggtc aggggtcact ttttcaaaag ctgctgtttt
    15241 aacgctgctt tgcaagtcac aatcagttac cacaatagga gtagaattgt ttttattgat
    15301 ttctgccaaa tcagcaattg catttcccca ggcggatcta ttgtcagttg gtttttcata
    15361 aagcatagga ttacccgatt tcagattaca ttgaagagca tatttatcct tagtgggctt
    15421 ttgctttact ggcttgaatt ctgctctcat cattttataa tcttccagcc ggttggattg
    15481 tccaagatag tgtaaggctt cttctaattg ggcttcattt aaagcagcac cgtgatattt
    15541 agcctgattt tccataaagg gaactccttt tcccatcaca gtatgagcta atatcatcaa
    15601 aggataatcc ttatctctgg tcatttgaag ggttgaaagt atctcttgat agttgtgacc
    15661 atcaatttct ataactttcc agccatccga ttcccagtta gctttgatat tttggggcat
    15721 cacggaatgg atatttccac ttatctgaag ttcgttataa tctacaatag cagtgagatt
    15781 attcagttga tatttaatag cgaatcttct tgcttccgag atttgtcctt tttgttgttc
    15841 tccatctccc atcaagacat acacattata gggaatatta tttattctgg aagcgagagc
    15901 aaatcctgct ccagcggaaa gaccctgtcc taaattacct gtactccatt ccacaccttt
    15961 cacatctctt tctatatgac cttcatagat gcttcctgaa agtctgaatt gagatataac
    16021 ctcatccaga ttatgataac cattcaaagc aagagcagta tacaccgcag gagaaatgtg
    16081 tccattgcta actactactc tatctctatc tggtaactca ggattttgag gattatgacg
    16141 gataaactgg tataaagtta ataataaatc aatggaggac atagaaccac caggatgccc
    16201 tgagccagct aaagtagtca tagtgagtat ggcacttctg gcaataatag cctgttcttg
    16261 taagcgggaa atcaattcta cctctgtcat ttgtaaacct catttaataa agaaagagcg
    16321 gtttgactcc tttttaagac aaaccgcctc aaaatttggt ggagcatagg aggctcgaac
    16381 tcctgacctc atgactgcca gtcatgcgct ctcccaactg agctaatgcc cct
//
LOCUS       DAORZF010000040        15932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_2988_length_15932_cov_384.970334, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000040 DAORZF010000000
VERSION     DAORZF010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 15932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15932
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2988_length_15932_cov_384.970334"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..2062
                     /locus_tag="PK015_05570"
     CDS             194..2062
                     /locus_tag="PK015_05570"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOL33970.1"
                     /translation="MGNTNNLITQLSLYYTTLQKVEEREEEDYQIRDYRRERTSKERN
                     IKEKQSAIDSQLVFNNLFQIIYEHPELKSFNLNSDDIAVISFFCYKISKEYQLEFSFE
                     EIIRIFPDKDWDFREQIEFIQSLLYREILSFSNYPLYGSAEDINFILYKHYSLNSLIF
                     HILLGKSPIKEARNIILNEYEKRPFIETVCYALNHLFDSYNELNLVDSECTMYYGRTI
                     NKLLSTALRVSHKLKSDNPYKIFVHTYGLDSFWQKCLLLIYKYNKSEKIIDYRILSAL
                     LSENKEQFNHFTEKILHNNPLQQAELLEKPGLFSSNGLVLSNSAIKELFEYHYEDIDI
                     RTYISSSPYFSLISPEQTLNQLILPADSFQSVVSIINQLKNPDLDTLASWGLITASLS
                     DGKETLNGCNILLYGEPGTGKTYIAGIIANELNKPLLKINTEAVRSHYYSDTEKSARK
                     LFKNMRQLHEKIAPVFLLNEGDSLMHCRNTYITTTVDSEENALQSVFLEELETFPGIL
                     IVTTNLRENLDYAIFRRFRYKLEIPVPDYDCRLKLWNLHLNDEIPGSAKIDLTSLAQE
                     FSLTGGEIRNVVLNACHHLISNPDLKELTYDILYYYARMESGSNLDKKNKEIGFRN"
     gene            complement(2130..2837)
                     /locus_tag="PK015_05575"
     CDS             complement(2130..2837)
                     /locus_tag="PK015_05575"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOL33971.1"
                     /translation="MAVLRDRHIVLWLDFLIPVAILVIGTWLIRNYFWDLKLSGLFYL
                     GNNNWWGMHNALCTFMYRYGTIPALVLSIAALGFFIVSFFAYNFYKWRRICLFLILVM
                     LLGPGLLVNGVLKEYWGRPRPRNIIPFGGKYVYEKPLEIDLSSPGSSFPSGHSAMGFY
                     FFTLYFLVRGKRKKWTALAFIIALLYGFFMGFVRIAQGGHFLSDILWSGGIVYLSSAL
                     CYYLLKINSTLELTSSQ"
     assembly_gap    3054..3153
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3154..5072)
                     /locus_tag="PK015_05580"
     CDS             complement(<3154..5072)
                     /locus_tag="PK015_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOL33972.1"
                     /translation="MLEHDQKQDLTDQKDENLLVGEGEPSKTNEEKTAPEGEISAATE
                     PEPELTIEMPELSEKPAEPVLEKSTEPNLTSEEEEKNNLKIDYSSPIPNLEPQQDIPI
                     TSIQSSSKEENDQLNQELLQGIDETIPQFKRGDIIEGTVVGINDQEVVVDIGVKNDGF
                     IPINEFEYSEVPQIDAKIKVLIEGAPNQDNRIPISKKKADFLINIEQIKKAAAEGETI
                     NGTISRRVKGGMIVDVMGIEAFLPGSQISNKSVPILDQFIGKEMSFKVIRIDENTHNI
                     ILSHKEVLEEEANQRRQELLSQIEVGMELDGEVKNITDYGAFIDLGGIDGLLHKSDMS
                     WGTLNHPSEMLNIGDKVKVKVIKFDPDSERISLGLKQLVPHPWENVEIKYPEGSKVSG
                     KVVSVQRFGAFVELEPGVEGLVHVSEMSWTKKIMDARKVLKVGDNVDAIVLEVDKENH
                     RISLGMRQMEPNPWLYIEDHYPIGSVITRPIKSLTPFGAFVEIEDGIDGLIHISDISW
                     TKRIYHPREVFRKGQEVEAVVLSIDRALHRIALGVKQLHPDPWENLAEALPVNTEVKG
                     KIGKLIPKGVLVDIKVGEYEVEGFVPLSHLAIPKLENTEDAFYVGEELPLKVIELDME
                     NRRLVLSVNAFFFSR"
     gene            complement(5066..5947)
                     /gene="ispH"
                     /locus_tag="PK015_05585"
     CDS             complement(5066..5947)
                     /gene="ispH"
                     /locus_tag="PK015_05585"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOL33973.1"
                     /translation="MQVFLAEHSGFCFGVRRAVKMAMEALNSGGEVCTYGELIHNPQI
                     VQKLEQEGISVCYDIKNLKDKKVVIRSHGIAREDLEILKANGNIIIDATCPYVKRAQE
                     LVSSVKELPILIMGDPDHPEVQALLSYGGKQTRIIKPGEKPESQMWETLCIVSQTTQK
                     LSNLQELVAQVLPHTLELRVFNTICNATTQRQEAALQLAKNSDLMIVIGGKHSANTRV
                     LQELCKQVTSSFHIETAAELTPDLLNGAQNIGLAAGASTPENIIVEVYNQIFRIKGEP
                     QFVQQIEEIPVYKEESC"
     gene            complement(5932..6624)
                     /gene="cmk"
                     /locus_tag="PK015_05590"
     CDS             complement(5932..6624)
                     /gene="cmk"
                     /locus_tag="PK015_05590"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOL33974.1"
                     /translation="MGKGLIIAIDGPAASGKSTTAQLLAKKLGYLYIDTGAMYRACAL
                     KAKKLGIDINDEESIRNLLAFIDIKIENDNSKNRILLDGEDVSEDIRADDISSLASAI
                     SAIPAVRYKMVELQRKMGEKGSVILDGRDIGTFVFPDAEVKFFLTASPEIRAKRRWLE
                     LQQKGIDKNFSEVLADLVKRDNNDSQRALAPLKKAEDAIEIDTSNMTIEEQTETLYKI
                     ILSKLEEECKCS"
     gene            complement(7067..8086)
                     /locus_tag="PK015_05595"
     CDS             complement(7067..8086)
                     /locus_tag="PK015_05595"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOL33975.1"
                     /translation="MRIAIIGGGGWGLALAKLLYENNNEILLWEYNDDFLETLIKTHS
                     NPQLLPDIILPSEISFTGDFDQLARFHPEIIILAVPTQFIRSTLQSIPREVQKELWNP
                     DRLLAIVNVAKGIEEHTLLTVSGILNQIIPSEVHNRICTLSGPSHAEEVARQIPTTVV
                     IAGSETETLKKLQLIFSNSYFRVYRSTDLKGVEMGGAVKNVIAIAAGIIAGLEYGDNT
                     IGALLTRGLVEIQRLGVACGARSETFLGLSGLGDLVTTATSQHSRNRYVGFQIGQGEK
                     LQDIVQSMAMIAEGVATTRSVYNLATQKNVEMPITEQVYQVLFLNKNPRKAILELMTR
                     DLKEE"
     gene            complement(8101..8487)
                     /locus_tag="PK015_05600"
     CDS             complement(8101..8487)
                     /locus_tag="PK015_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33976.1"
                     /translation="MEFRKEKDTKKILEFIELSENYKIELRRYREPVLVWAVEDGTAY
                     YSIWENEMLEKFGLENVDGWDYEEDLLFCPDWMADEEDKLDDDEDDDEDLGWDMKKTT
                     PRKKKSTSTKSTTQKTTSTKSSAKKK"
     gene            complement(8497..9630)
                     /locus_tag="PK015_05605"
     CDS             complement(8497..9630)
                     /locus_tag="PK015_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33977.1"
                     /translation="MSDFPSKNDPKIVAPSDNTSSKPQPQKDKNKDSSKNKSKHPTNR
                     KVIPIIMSLILVLLLSKTTLNAQMEVSISTGITYSDNVFHLSDYDINRWKQGHPNLDF
                     VNTTDDLTLMLQADLAYPFRYQWWKFIPSVTAKVSQNISNPDKQRQDILMRFRVERYY
                     WNFTALYGYYPYIYVRNYIDSDGTGESEKYSYERNLYRGDLNVKPFKNTTIQFHGRYE
                     EYFYNQYWTEYDANATTLGLGARYSFPAFSMGASYNWRVLDNYNHDAEDASYESDIYK
                     GDIRLKKTPLSASKPKGAAFYPELAMSYEERFYQSDDPRYGGRVDRIYNTNAALNFLL
                     NEQWNIKLDYTHTFRNIESPVEEIRLTKEYSENKFSLTAKYSF"
     gene            complement(9640..10455)
                     /locus_tag="PK015_05610"
     CDS             complement(9640..10455)
                     /locus_tag="PK015_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33978.1"
                     /translation="MLKRFFVTIAIVLGITIIIAKVQPLDFENPGNRRLLKTEEGNYW
                     YYRSQPEKSMFLNVEGISTIYLRSFSVAKVHKPKVIISFGKDKKTYDLTLKEQKDGFY
                     IYDEITIPVPEGAKKMELLCYERSIYFRPFYTPAPKTKPKSSKPPNLVVKAHGGIINI
                     SHNGTESQYYTFNSSQSFKFTLNNGRNAIVYVRARLHDNTLPIFELYRNGELVDSYEF
                     SLQRSTKYKAPGVNYLSIGKKIDLPPNSGTTEYELRAKSDHMFFARPVLLKQN"
     gene            complement(10468..11589)
                     /gene="pgsW"
                     /locus_tag="PK015_05615"
     CDS             complement(10468..11589)
                     /gene="pgsW"
                     /locus_tag="PK015_05615"
                     /inference="COORDINATES: protein motif:HMM:TIGR04332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate system protein"
                     /protein_id="HOL33979.1"
                     /translation="MYRPSLKSGKSLTLLFFLSIILYLIASNSYVNIKSENYELKVAA
                     AKEMAAFMDTIKAEIQRQGLPIDPINDPFQTGLIGRARTTITTDRGLLSDKQAALNPN
                     LAAVFVEEFTKQGLKEGDYIAVGITGSNPGINLALYAAIKVMKLKPSIIVSLSSASYG
                     ANQEEFTWLDMEGVLRRKGMIDFGSSYASLGGKDDLGVGLSDQGIKAMLQAMARNGVS
                     PLIGATLEDNVKAREIAYDQMLPEGARYKLFVNIGRGLANVGSEPNANQMVEGLNPKL
                     AEESFEPEGIMMVMAKKGVPVFNVQRIQRWIKKYHLENDSGQIPKPGVGPAFSVEKHN
                     VTVAAICLAILIAAIVAVIILDRNDRRFMANIVDPDEDL"
     gene            complement(11591..12046)
                     /gene="pgsC"
                     /locus_tag="PK015_05620"
     CDS             complement(11591..12046)
                     /gene="pgsC"
                     /locus_tag="PK015_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein PgsC"
                     /protein_id="HOL33980.1"
                     /translation="MVESIIQAAVGLGVIISLLFSELLGASAGGIVVPGYIAMYLDRP
                     MQILGTIVVSLLTWGIIRLISNFTLLFGKRRMVLSILVGFILGWASRLLVYQNVTVYT
                     YQLQSIGYIIPGLIANWFERQGFWKTITSLGVAAVLVRLILMVVFKGEL"
     gene            complement(12031..13230)
                     /gene="pgsB"
                     /locus_tag="PK015_05625"
     CDS             complement(12031..13230)
                     /gene="pgsB"
                     /locus_tag="PK015_05625"
                     /inference="COORDINATES: protein motif:HMM:TIGR04012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate synthase PgsB"
                     /protein_id="HOL33981.1"
                     /translation="MNVLIVATVLLVGYWIYEYWRHQRNVNAIPIRIHVNGTRGKSSV
                     TRLIAAGLRAGGKRTVAKTTGTTPRVILPDGREAAIIRLMGANIIEQKYIFRYAAKEH
                     PDAVVIECMAVNPAYQWITERKFVRSTVSVITNCRPDHLDVMGPNEENVAMSLSNTIP
                     SNGICYTAEDKYFPLLKKVAKQRNCKIYQIRPTDVTEEELSKFNYIEHAENVQLALAV
                     CAELGIPRHIALEGMQNSHPDPGALKKYVIYDEEKTLYFYNIFAANDPDSTARIINMV
                     TGHLENVEKIIILNSRADRVFRSQQLLEAVSKLDFSYILLTGELPDKIAAYAHHLGIP
                     DEKVIPLGEPSPEVIYNKVLNLTEKEAHILGIGNIAGRIKYGGQIVAYFKHKSKQQYE
                     DLKWLNQ"
     gene            complement(13613..15631)
                     /locus_tag="PK015_05630"
     CDS             complement(13613..15631)
                     /locus_tag="PK015_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HOL33982.1"
                     /translation="MPKAFNYVIIIICLTVMPAILLAHQYQNEQWDIDFSRFTETPPP
                     VAPVRPVAEFEPASQVLIRYPLGIPVSLVVQLANTAQVICLVSSSSTQNAATNAFLNA
                     GVNMDNVTFMIASTDSYWTRDYGPWFIFDGNGDLGVVDFQYNRPRPNDNLIPQIFANQ
                     QGLPYYGMNLMQTGGNYMNDGINTAAQTNLVYSENSSLSQQEVYNRMHNYLGIENYYV
                     LADPNNTYIDHIDCWGKFLAPDKILIRSVSPSHSQYNAIEQVASYFSSTLCAWGYPYK
                     VYRVYTPQDQPYTNSLILNNRVFVPIMNSTYDAAALQTYRDAMPGYEVIGVPAASNAP
                     WESTDALHCRTHEIPDKNMLEIVHNPYFGSYANYSNWQINANIIARSRQALYPDSVFV
                     CYKVNQSPWQNAIMSQSSGNLFEVILPAFAPGDTIRYFIHAADESGHSRNHPVFAALE
                     PHLFVCAPDTEAPIIYHNPPTSIQSGEHTFFAIVTDNLEVSNVVFRYKIDEGEVTELN
                     MTYQPEIQPDYWTITLNLTFNEQDEIFHYQIEARDNANPCNITCYPWLGEWLNIPIDH
                     TGIGDDSIPSVQKSELFAYPNPYFLQKNISLNIVSKNINNEQSSLKIFNSRGQLVKSL
                     SIAESSTEFTKYQWDGKDSKGNKVDSGIYFLKWDNRDKTLIHKILVIK"
BASE COUNT         4921 a         3088 c         2805 g         5018 t
ORIGIN      
        1 gctaaagtta gtaataaatt ggaaatagat aaattatggt ttatgtccgc taagggagag
       61 cttagttaga gtcataatca aatcataaat aagcagttag cgcaccaaat ccaaaaaaat
      121 ttaaaatttt tccgctattt cgtttttttc ttgttataat agggtatggt agaaaaaaat
      181 aagtggagga ataatgggca atacaaataa tctaattact cagcttagcc tttattacac
      241 aactcttcaa aaagtagaag aaagggaaga ggaggattat caaattcgtg attataggag
      301 agaaagaacc tcaaaagaaa ggaatattaa agaaaaacaa tcagccattg atagccaact
      361 ggtctttaat aacttgtttc aaataatata tgaacatcca gaattaaagt catttaatct
      421 aaatagtgat gatatagcag taatttcatt cttttgctat aaaatttcta aagaatatca
      481 actggaattt tcttttgaag aaataatcag aattttccct gataaagatt gggattttag
      541 agagcaaatt gaattcattc aaagcctgtt atatagagaa atcctgagtt ttagtaatta
      601 tcccttatat ggctcagctg aggatataaa tttcatactt tataaacatt acagtttaaa
      661 tagcctaatt tttcatattc tactgggaaa atcccccatc aaagaagcca gaaatattat
      721 tctcaatgaa tatgaaaagc gaccctttat tgaaacagtc tgttatgcac taaaccattt
      781 atttgatagt tataatgaat taaatctggt tgatagtgaa tgtactatgt attatggtag
      841 aaccataaat aaattattat ctacagcttt gagggtttct cataaactta aatcggataa
      901 tccctataag atttttgttc atacctatgg attagattct ttctggcaaa agtgtctgct
      961 cttaatatat aaatataaca aatccgagaa aatcattgat tatagaatat tatctgcttt
     1021 gctttcagag aataaagagc aatttaacca ctttacagag aaaatactac ataataaccc
     1081 gctacagcaa gcggaattac tggaaaaacc aggactgttt agctcaaacg gacttgttct
     1141 gagcaattct gctataaaag agttatttga ataccattat gaggatatag atatcaggac
     1201 ttatattagt tcttcaccct atttttcgct catttcacct gagcagaccc taaatcaact
     1261 cattttacct gcagatagtt ttcaaagtgt agtttctata ataaatcagt tgaaaaatcc
     1321 agatttagat accttagcca gctgggggtt aattactgca tccttatcgg atggaaaaga
     1381 aactctaaat ggatgtaata ttctgttgta cggtgaacct ggtacgggaa aaacttatat
     1441 tgctggaatt attgccaacg aactgaacaa gcctctatta aaaattaata cggaagcagt
     1501 tagaagccat tactatagtg atacagaaaa atccgccagg aaattgttta aaaatatgcg
     1561 acagctacat gagaaaatag cgcctgtttt tttactcaac gaaggtgata gtctgatgca
     1621 ctgccgaaat acttatataa caactaccgt tgatagtgaa gaaaatgctc tgcaaagtgt
     1681 atttctggaa gaattggaaa ctttccccgg aattttaata gtaactacca atttgagaga
     1741 aaaccttgac tatgcgatat tccgtcgttt ccgttataaa ctggagatcc ctgttcctga
     1801 ttatgattgt cgcttaaaac tctggaattt acatctaaat gatgaaattc caggttctgc
     1861 taaaattgat ttaacttctt tagctcagga attctctctc acgggaggag agataagaaa
     1921 tgttgtcctt aatgcctgtc atcacttaat atctaatcca gacttaaaag agttaaccta
     1981 tgatatttta tattattatg cccggatgga atctggcagc aatctggata agaagaataa
     2041 agaaattgga tttagaaatt gaggtatgaa attaaagctt tccttaacct tttattataa
     2101 tataaccata ccttaaaggg agatgaatat tactgagagg aggttaactc taaggttgaa
     2161 ttgatcttta acagataata acagagagcg gaagaaaggt agactatccc tccggaccat
     2221 aatatatctg agaggaaatg acctccttga gcgatgcgta caaaacccat aaaaaaacca
     2281 tacaggagag cgataataaa tgcgagagcg gtccattttt ttcttttccc gcgcaccagg
     2341 aaatatagag tgaagaaata gaaccccata gcagaatgac cactgggaaa ggaacttcct
     2401 ggactggaaa ggtctatttc taaaggtttt tcatagacat attttcctcc gaatggaatt
     2461 atatttcttg gtctgggacg accccaatat tctttcaaaa ctccatttac gagtaatccc
     2521 ggtcctaaca acattaccag tatcagaaag aggcagatgc gacgccactt atagaaatta
     2581 taagcgaaaa aactaactat aaagaatccg agagctgcaa tagagagcac aagagctgga
     2641 atcgtgccat agcgatacat aaaagtgcac aaagcattat gcattcccca ccaattatta
     2701 ttacccaaat aaaacaatcc tgagagtttg aggtcccaaa agtagttacg aattagccaa
     2761 gttccgatta ccagaatagc aaccggaata agaaaatcca gccacaaaac tatatggcgg
     2821 tccctaagaa ccgccatatt tattttattt ctcatagtct ttaatgagca taatttaatc
     2881 tttaattaaa gttctttctg aacatagttt aagaaacagt cctattcaga tttagtttct
     2941 atatcctgtt ctttcttttc ttctgtttca gtttctttag taataacact ttcagcttct
     3001 tcttcaacag caggctctgg attttctgaa gtctcaggtt ctggttgttc ctcnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntcacgag agaagaagaa agcgttcaca
     3181 gaaaggacta atcttctatt ttccatatcc agttcaatga ctttaagggg taattcctct
     3241 cccacataaa aggcatcttc ggtattttca agttttggta ttgccagatg agaaagtggc
     3301 acaaatcctt ccacttcata ttctccaact ttaatatcca ccaaaacacc tttcggaatg
     3361 agtttaccaa tcttaccttt aacttcagtg ttaacaggta aagcttcagc taaattttcc
     3421 cagggatcgg ggtgaagttg ttttacaccc agagcaatcc gatgtaaagc acggtctata
     3481 gaaagcacta cagcttcaac ttcctgacct ttacgaaaga cttcccgggg atgataaatc
     3541 cttttagtcc agctaatatc ggaaatatga atcaagccat ctataccatc ttcaatttcc
     3601 acaaaagcac caaatggagt aaggctcttt atgggacgag taatgacact tccaattgga
     3661 taatggtctt ctatgtagag ccagggattg ggttccatct gtcgcattcc aagggagata
     3721 cgatgatttt ctttatccac ttccaaaact attgcatcaa cattgtcgcc cacttttaac
     3781 acttttctgg catccataat ctttttagtc cagctcattt ctgaaacatg aaccaagcct
     3841 tcaacaccag gttccagttc tacaaatgca ccaaatcttt gaacactcac aactttcccg
     3901 gaaactttac taccttcagg atatttaatc tctacatttt cccagggatg aggaaccaat
     3961 tgttttaatc cgagagaaat cctttcgctg tcagggtcaa atttaatcac tttaacttta
     4021 actttatcgc caatattgag catttcactg ggatggttca gagttcccca gctcatatcg
     4081 gatttatgaa gaagtccatc aattccgcct aaatctatga aagctccgta atcagtaata
     4141 tttttgactt cgccatcaag ttccatccct acttcaatct gggagaggag ttcttgtctt
     4201 ctttgattag cttcttcttc caacacttct ttatgtgaaa gaatgatatt atgtgtattt
     4261 tcatcaatac gaatcacttt aaaactcatc tctttcccta tgaattggtc aaggattgga
     4321 acagatttat tggatatctg agagccaggc aaaaatgctt caattcccat tacatcaact
     4381 atcattccac cttttactct tctactaatt gttccattga tggtttcccc ttctgcagcg
     4441 gctttcttta tttgttctat atttatcaag aaatcagcct ttttcttgga aatagggatt
     4501 ctattatcct ggttaggtgc tccctcaatt aacactttaa ttttagcatc aatttgagga
     4561 acctcgctat attcaaattc attaattgga ataaagccat catttttgac cccaatatcc
     4621 acaaccactt cctggtcatt aattcccaca actgtacctt caataatatc ccctcgttta
     4681 aattgcggta tagtctcatc aattccttgc aagagctctt gatttagctg gtcattttct
     4741 tcctttgatg aactttgaat agaagtgata ggaatatctt gctgtggttc caaattggga
     4801 ataggagatg aataatctat tttcaaatta ttcttttcct cttcttcgct tgttaaattt
     4861 ggttcagtgc ttttttccag cactggttca gccggtttct ctgatagttc cggcatttca
     4921 atggtcaatt ctggttccgg ttctgttgcg gcagaaattt ccccctcggg tgccgttttt
     4981 tcctcattag ttttcgacgg ttcaccttca cccaccaata aattttcatc tttttggtcg
     5041 gttaggtctt gtttttgatc atgttctaac atgattcctc cttatacacc gggatttcct
     5101 ctatctgttg aacaaattga ggttctcctt taattctaaa aatctgatta taaacttcta
     5161 caataatatt ttctggagtg gaagcacctg cagctaaacc tatgttctga gcaccattta
     5221 ataaatcagg tgtcaattct gcggctgttt caatatgaaa actgcttgta acttgtttac
     5281 atagctcctg cagaactcgc gtattagcac tgtgctttcc acctataaca atcattaaat
     5341 cgctattttt tgccagttgt aaagcagctt cttgccgctg agtggtggca ttgcaaatag
     5401 tattaaatac tcgtaactct aatgtatgcg gtagaacttg agctacaagt tcctgaagat
     5461 tacttagttt ctgcgtagtc tgactgacaa tgcagagggt ttcccacatc tgggattctg
     5521 gtttttctcc cggtttgatg attcttgtct gtttgccgcc ataagacaat aaagcttgaa
     5581 cttcaggatg atcaggatct cccataatca aaatcggtaa ttctttcaca gaagatacca
     5641 gctcttgcgc tcttttcaca tagggacaag tggcgtcaat aattatatta ccattcgctt
     5701 ttaaaatttc caaatcttcc ctggcaatgc catgagaacg gatcactacc tttttatctt
     5761 taagattttt aatgtcataa caaacggaaa tgccttcctg ttcaagtttt tgcactatct
     5821 gaggattgtg aatcaattca ccataagtgc agacttctcc gcctgagtta agagcttcca
     5881 ttgccatttt aaccgctctg cgaacaccaa aacagaagcc agaatgctca gctaagaaca
     5941 cttgcattct tcctccagtt tggaaagaat aatcttatac agggtttccg tttgttcttc
     6001 tatagtcata ttgctggtat caatttctat ggcatcctct gcttttttga gaggcgccag
     6061 agcacgttga gaatcattat tatcgcgttt taccaggtcc gctaaaacct cggaaaaatt
     6121 cttatctata cctttttgct gaagttccag ccatcttctc ttagctctaa tctctggact
     6181 ggcagttaaa aagaacttaa cctcagcatc gggaaaaaca aaagtgccaa tatcccgtcc
     6241 atcaagaatt acacttcctt tttctcccat ttttcgctga agttctacca ttttataacg
     6301 aacagctgga atggcagaaa tagcagaagc aagagaagaa atatcatccg ctctaatatc
     6361 ttctgagaca tcttccccat ccagaagaat tctgttttta gaattgtcgt tttctatctt
     6421 aatatctata aaagccaata aatttcggat agattcctca tcattgatat caatacccag
     6481 tttttttgct ttaagagcgc aagcacgata catagctccg gtatcaatat acagataacc
     6541 taatttttta gccaggagct gagctgtggt gctttttcca gaagctgcgg gtccatcaat
     6601 agcaataata agtccctttc ccatatcttt ttacctgaaa atccaatttt tttgctctct
     6661 ttatggcgtc aatctttttt atgctttcag ttaagaaatg gaaaaaataa ctttcattgt
     6721 cttttaattt ttttagctcg tatctcccct tttcttacgg actaatgtcc agcttatgtc
     6781 cagcttatgt ccagcttatg tccagcttat gtccgatagt ggacataagc tccatataag
     6841 ataagcatca ataagagatt aaaagggatg aaagatagag aggggttata tttcaaagaa
     6901 aagcagattc caataattga ttaaggagtt aatctatctt ttaacaaatt aattaattaa
     6961 agacaaaagg gagttagtag atagattttt gaagttgtaa agcttatcct cttgataaaa
     7021 ggtaaaaaaa cttaatctat aaaattaaaa aaattgtaaa ctatatttat tcttccttta
     7081 aatccctggt cattagttcc agaattgcct tacgaggatt cttattcaga aaaaggactt
     7141 gataaacctg ttccgtaatc ggcatttcta catttttctg agtggcaaga ttataaactg
     7201 aacgagtagt ggcaactcct tccgctatca tagccatact ctgaactata tcctgcagtt
     7261 tttccccctg tccaatttga aaaccgacat accgattacg gctatgttga gaggttgcag
     7321 ttgtcacaag atctcccaga ccagataatc caagaaaagt ttcagagcgt gcaccacaag
     7381 caacacccaa cctttgaatt tccactaagc cacgagttaa taaagctccg atagtgttat
     7441 ccccatattc cagaccagca atgattccag ctgcgatagc aataacattt ttaactgctc
     7501 ctcccatctc tactcccttc aagtccgtac tccgatacac ccgaaaataa ctgttactga
     7561 aaataagctg taatttttta agagtttctg tctcagagcc agcaatgact acggttgtgg
     7621 gaatctgacg agctacttct tcagcgtgac tgggacctga aagagtgcat atccgattat
     7681 gaacttcaga aggtataatc tgattcaaaa ttccacttac ggttaaaagt gtatgctctt
     7741 caatgccttt ggcgacatta acgatagcca aaagtcggtc aggattccat aattctttct
     7801 gtacttccct gggaatagat tgcaaagttg atcttataaa ttgagtaggc accgcaagaa
     7861 ttattatttc aggatggaac cgagcaagtt gatcaaaatc accggtaaac gaaatttcag
     7921 aaggtagaat gatatcaggt agaagttgag ggttagaatg tgtctttatc agcgtttcaa
     7981 gaaagtcatc attatattcc cagagcagta tttcattatt attttcatag agaagtttag
     8041 ccagagccag accccaacca cctcctccaa taatagctat tcgcatcttt gtaaaaaaat
     8101 ttactttttc ttagctgaag atttagttga ggttgttttc tgagtagtgg atttagtaga
     8161 agtagatttc tttttcctgg gagtagtttt cttcatatcc cagccaaggt cttcatcgtc
     8221 atcttcatcg tcatccaatt tatcttcttc atcagccatc caatctggac agaaaagtaa
     8281 gtcttcttca taatcccagc catcaacatt ttccagacca aacttttcca gcatttcatt
     8341 ttcccagata gaataatagg ctgttccatc ttctactgcc caaaccagaa cgggttcacg
     8401 gtaacgacgg agttcaatct tataattttc cgataattct atgaactcca gaatcttttt
     8461 agtgtccttt tctttacgaa attccataac acccacctaa aaactatatt tagcggtaag
     8521 agaaaatttg ttttcactgt attctttagt taaacgaatc tcctccacag gtgattcaat
     8581 attcctgaat gtatgagtgt agtcaagttt tatattccat tgctcattca agaggaaatt
     8641 aagtgctgca ttggtgttat aaattctatc tactctacca ccataacggg ggtcatccga
     8701 ttgataaaat ctttcctcat aggacatagc caattcagga tagaaggctg ctcccttagg
     8761 tttggaagcg gaaagaggag ttttttttaa gcgaatatcg cctttataga tatcactttc
     8821 ataactggca tcttcagcat catgattata gttatctaaa actcgccaat tatagcttgc
     8881 tcccatactg aaagcaggaa aagaataccg agcacctaat cccagagtgg tagcattggc
     8941 atcatattct gtccagtact gattataaaa atactcttca tagcgaccgt gaaactgtat
     9001 ggtggtattc ttaaaaggtt tcacattaag gtctcctcta tagagatttc gttcatagct
     9061 atatttttcc gactcaccag ttccgtcaga atcaatatag ttgcgcacat agatgtaagg
     9121 ataatagcca taaagggcag taaaattcca ataatagcgt tccacccgga agcgcattag
     9181 tatgtcttgg cgctgtttat ctggattgga tatattctgt gaaaccttgg cggttactga
     9241 agggataaat ttccaccatt gataacgaaa aggataggca agatccgctt ggagcataag
     9301 ggtcaaatca tctgtagtat taacaaaatc cagattagga tgaccttgtt tccagcgatt
     9361 tatatcgtaa tcagaaagat ggaacacatt atcggaatag gttattcctg tagaaataga
     9421 gacttccatt tgagcgttaa gggttgtttt acttaaaagc agaaccaaga ttagactcat
     9481 aataatgggt ataactttac gattagtggg atgtttggat ttatttttgg aggaatcttt
     9541 atttttatcc ttttgaggtt gaggtttaga tgaagtatta tctgaaggag ctactatttt
     9601 tgggtcattt tttgatggaa aatcagacat acttaactct caattctgtt ttagcaatac
     9661 tggtctggca aagaacatat ggtcggattt agctctgagc tcatattccg ttgttccact
     9721 atttggtggc aaatcaatct tctttccgat agaaagataa ttcactcctg gagctttata
     9781 tttggtgcta cgttgaagtg aaaattcata actatccacc aattcaccat tccgataaag
     9841 ttcaaatatg ggcagagtat tatcatgtaa tctggcacga acatagacaa ttgcattacg
     9901 accattattc aaagtaaact tgaaactctg ggaagaatta aaggtgtaat actgactttc
     9961 agtgccatta tgcgagatat taataattcc gccatgagct tttactacga gattaggggg
    10021 tttagaactt tttggtttcg tcttgggagc aggagtgtag aaaggtctaa aatagatact
    10081 tcgctcataa caaagcaatt ccattttctt ggctccttct ggcactggta tagttatttc
    10141 atcatagata tagaaaccat ctttctgctc ttttaaagta aggtcatagg tttttttatc
    10201 ttttccgaag ctgataatga ccttaggttt gtgcaccttt gcaacggaaa aagaacgcaa
    10261 ataaattgta gaaatccctt ctacattaag aaacatagat ttttccggtt gcgaacgata
    10321 ataccagtag ttaccttctt cagtttttag taaacgacgg ttccctggat tttcaaagtc
    10381 cagcggttga actttcgcaa tgatgatggt tattcctaaa actattgcta tggtaacaaa
    10441 aaagcgtttt aacattactg ctccaaatta caaatcttca tcagggtcta ctatattagc
    10501 cataaatcta cggtcgttcc ggtccagaat aatcactgcc actattgctg cgataaggat
    10561 agctaaacaa attgcggcta cggtaacatt atgtttttca acactgaaag caggaccgac
    10621 gccaggtttt ggtatttgac ccgaatcatt ttccagatgg tattttttaa tccagcgttg
    10681 gattcgttga acattaaata caggaacacc cttttttgcc atcaccatca ttataccttc
    10741 aggttcaaaa ctctcttctg ccagtttggg atttagaccc tctaccatct gatttgcatt
    10801 aggttcactt cccacattag ccaatccacg accgatattt acaaagagtt tatatcgcgc
    10861 accttctggt aacatttggt cataggcaat ttctctggct ttaacattat cctctaaggt
    10921 agcaccaatt aagggactca ctccatttct tgccattgcc tgaagcatag ctttaatccc
    10981 ttgatcagaa agccctactc caaggtcatc tttaccacct aaagaagcat aagaactgcc
    11041 aaaatctatc atacctttcc gacgcaggac accttccata tccagccagg taaattcttc
    11101 ttgatttgct ccataggaag cagaagaaag agaaacgatg atggaaggtt tcagtttcat
    11161 cacttttatg gctgcgtaaa gtgccagatt aatcccggga ttggaaccgg tgattccaac
    11221 tgcaatataa tccccttctt tcaagccttg tttagtaaat tcttccacga aaacggcagc
    11281 taaattggga tttagcgcag cttgcttatc ggataaaaga ccacggtcag tggtgatagt
    11341 tgttcttgct cttccaataa gaccagtttg aaaagggtca tttatagggt caataggcaa
    11401 gccctgtctt tgaatttccg ctttgatagt atccataaaa gcagccattt ccttagctgc
    11461 tgcaactttc aactcatagt tttcgctttt tatatttaca taactgttgc tggcaatgag
    11521 ataaaggatg atactcaaga aaaagagaag ggtgagtgat ttgccagatt tgagtgaagg
    11581 acgatacatc ttacaactct cctttaaaga caaccatcaa gataagacga accagaactg
    11641 ctgctactcc cagagaggta atagttttcc aaaatccctg tctttcaaac cagtttgcta
    11701 ttagaccagg gataatataa ccaatggact gaagttgata ggtgtagacg gttacatttt
    11761 gatagactaa tagacgagaa gcccacccca atataaaacc taccaaaatg cttaaaacca
    11821 tccttctctt gccaaataga agggtaaaat tgcttataag ccggataatt ccccaagtga
    11881 gcaagctaac cactatggta cctaaaatct gcattggacg atccagatac atagctatgt
    11941 atcctggaac gacaatacct ccggcagaag cacctaaaag ctcactaaaa agtaggctta
    12001 taatgacacc aagacctact gctgcctgaa ttattgattc aaccacttta agtcctcata
    12061 ctgctgtttg gatttatgct tgaaatatgc cacaatctga cctccatact taattcttcc
    12121 tgctatattg ccgattccca gaatgtgagc ttccttttca gttaagttta gcactttatt
    12181 gtatattact tctggtgagg gttctcccag aggaataacc ttttcatctg gtattcccag
    12241 atgatgagca taagcggcaa ttttatcagg aagttcaccg gttagtaata tataggagaa
    12301 atccagttta cttactgctt ctaaaagctg ttgagagcgg aaaactctat ctgcacggct
    12361 atttagaata ataatctttt ccacattctc caagtgtcca gtgaccatat ttatgatacg
    12421 agcagtggag tcaggatcat tagcggcaaa aatattataa aaatagaggg tcttctcttc
    12481 atcatagata acatactttt tcaatgcacc tgggtcagga tgagaatttt gcatcccttc
    12541 cagagcgata tgacgaggaa ttcccagttc tgcacatact gccagagcta actggacatt
    12601 ttccgcatgt tctatatagt taaatttaga aagttcttcc tctgtaacat cggtaggtct
    12661 aatttgatat attttgcaat tgcgttgttt ggctactttc tttaacagag gaaagtattt
    12721 atcttctgca gtatagcaaa tcccattgga aggtatagta ttggataggg acatagccac
    12781 attttcttca ttaggaccca tcacatcaag atggtcagga cggcaattgg taataacact
    12841 aaccgtacta cgaacgaatt ttctttctgt tatccattga taggcaggat taactgccat
    12901 acattcaata actactgcat caggatgttc tttagcagca taacggaaaa tatatttctg
    12961 ttctataatg ttagctccca ttaaacggat tatagcagct tctctaccat caggaagtat
    13021 aactcgggga gtggttccag tagttttagc aaccgtcctt ttaccaccag cacgaagtcc
    13081 tgcagcaatg agacgggtta cactggattt acctcgtgtc ccattaacat gaatccgaat
    13141 aggaatagca ttgacattgc gttgatgccg ccagtattca taaatccagt atcctacaag
    13201 tagcaccgtt gcaactatca gaacattcat ttatttcctc ttaattattt acatcactca
    13261 gatagaaaaa ctctatttta cgcttagtat tctttaatgc aaaatccgtt ccaccctttt
    13321 ttcttttctc taatttgttt gaaggatgga ttaaaggcta agaaatttat attggatttt
    13381 aggcaatctt ttatttactt tatagataga gaagaggaga tatttaaata aaaagaaaaa
    13441 aggctacata aaaaaaatac agtggggaat gccgggcggc atccccactg ctcggagggc
    13501 attctatgta gggtctgtag gctacaatca cttaaaataa tattaataat aatgtcaaga
    13561 gaaaagtatg gataattttc atctttatct gttgatatat ttccacagct ctctatttaa
    13621 tgactaatat cttatgtatt aaggttttat ctctattatc ccacttcaag aaatatatgc
    13681 ctgaatctac tttattacct ttgctatctt tgccatccca ttgatatttc gtaaattccg
    13741 tagaactttc ggcaatagag agacttttta cgagctggcc tctgctgttg aaaattttca
    13801 acgaactctg ttcattattt atatttttac tcactatgtt aagagaaata tttttctgca
    13861 agaagtaagg attaggatag gcaaataatt cagatttttg cactgaagga atagaatcat
    13921 cccctatacc ggtatggtct atagggatat ttagccattc acctaaccag ggatagcaag
    13981 taatattgca gggattggca ttatctctgg cttcaatttg ataatgaaaa atctcatctt
    14041 gctcattaaa ggttaaattc aaagtaatag tccagtagtc aggttgaatt tcaggttgat
    14101 aagtcatatt taattcagtt acttcaccct catctatttt ataccggaag accacattgc
    14161 taacttctaa gttatctgtc actatagcaa agaaagtatg ttcaccactt tgaatggagg
    14221 taggcggatt atgatagatt atgggagctt cagtatctgg agcacagaca aataagtgcg
    14281 gttccagagc ggcaaaaaca ggatgatttc tactatgccc agattcatct gctgcatgta
    14341 taaaatagcg aatagtgtca cctggtgcaa aagcagggag tattacctca aacaaatttc
    14401 ctgaactctg gctcataatg gcattttgcc agggactctg attaacttta tagcaaacaa
    14461 atactgaatc aggatagagt gcctgtctac tacgagcaat gatattagca tttatttgcc
    14521 agttagaata attagcgtaa ctaccgaaat agggattatg aacgatttcc agcatatttt
    14581 tatcgggaat ttcatgagtt ctgcaatgta aagcatcggt agattcccag ggagcattac
    14641 tggcagcagg aacaccaata acttcataac cgggcattgc atcacgatag gtttgcaaag
    14701 ctgctgcatc atatgtgcta ttcataatag gaacaaacac tctattattg aggattaagg
    14761 aattggtgta aggttgatct tggggagtgt aaactctgta aactttataa ggataacccc
    14821 aggcacaaag agtagaagaa aaataactag cgacctgttc aattgcatta tactgagaat
    14881 gcgaaggaga aacactacga atcaggattt tatctggagc taagaatttt ccccagcaat
    14941 caatatggtc aatataggta ttattggggt ctgcaagcac atagtaattt tcaattccga
    15001 ggtagttatg cattctatta taaacttcct gctgggataa agaagaattt tctgaataaa
    15061 ctaaattggt ttgtgcagca gtatttatac cgtcattcat atagttcccc ccagtctgca
    15121 tcagattcat accataatag ggtaatcctt gttgattggc aaaaatctga ggaatgagat
    15181 tatcattggg acggggacga ttatactgaa aatccaccac acctaaatca ccattgccat
    15241 caaagatgaa ccagggtcca taatctctcg tccaataaga atcagtactt gctatcataa
    15301 aggttacatt atccatattc actccagcat taaggaaagc attggttgca gcattctggg
    15361 tagaagaaga tgaaaccaga catatgacct gagcagtatt ggctaattgg acaaccagtg
    15421 agacgggaat tccgagagga tatctaatca ggacttgcga ggcaggttca aattcagcca
    15481 ctggacggac aggagcgaca ggtggaggag tttctgtgaa acgactgaaa tcaatatccc
    15541 attgttcatt ttgatattga tgtgccaaaa ggatagctgg catcacagtc aaacaaatta
    15601 ttattatgac ataattaaaa gctttgggca ttacaatttc tccccaatca agaaagatac
    15661 tttattaaat atacaatttt tatgcctaaa atagagaaat ctttttttgt ctactggaaa
    15721 aataatagtt taactatatc cttaatcata gtggaatatg gtggaacaag tatggtgtaa
    15781 ggggtattgg tggaacgagg tcctcctcgt ttcaagctcc gaaggagctt tcttttttgg
    15841 ggtcataaac gtccccttac acggggtcat aaacgtcccc gtttatgaag ctccgaagga
    15901 gcttgctatt ataaagagat aataacatta at
//
LOCUS       DAORZF010000041        15292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_3119_length_15292_cov_360.502264, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000041 DAORZF010000000
VERSION     DAORZF010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 15292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15292
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3119_length_15292_cov_360.502264"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..2173
                     /gene="fusA"
                     /locus_tag="PK015_05635"
     CDS             119..2173
                     /gene="fusA"
                     /locus_tag="PK015_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOL33983.1"
                     /translation="MKEYELKKMRNLLFMGASGSGKTSLAEQIFFLTHTTNRLGKIDE
                     GNTVMDFDPEEISKKMSLGLSVGYAHHKDCKINIIDVPGSPDFIGETIVSIPAVENVV
                     LVVNGTAGYEVGLELAIEQLENEKAAKIVLVNRMDNEHADFQKTLDTIYENAGFNPIP
                     VHIPIGKESTFEGVVDVIRQKAILPGGEKDIPADMQSTIAEAHQKLMEAIAETDEELL
                     NDFLEKGELSEEQLINGMRKALANGEICVAFACSASTGVGVMAFLDAVEKYLPSPEDV
                     NPISVLRKGEPAEFIASPDGELLGYVFKLYADPTMGDYAYLRLFSGSLKTGMDFSIPE
                     KGVKERAGNIYYMLGKNREDAQELKAGEIGALVKLKNTRALNTITAPGSDLSLKMPEL
                     PTPTYWQAIKALNQSDEEKISNALQKVIAEDPTIRFEVNQETHENILSGLGEQQLQLV
                     LRKLKNRYKVDAFLQDPRIPYKETITTSGEAQYRHKKQTGGRGQYAEVYFRIKPLERG
                     EGFKFINSIVGGVIPSNFIPAIEKGVVETMEKGIIAGYPVVDVSVEVYYGSYHEVDSS
                     EMAFKIAASMCFKEGFKKCKPILLEPIHELTVIVPTEYMGDVMGDISTRRGRIMGMEQ
                     HGKKQHLIVQMPVAEMYSYYPALKSLTQGRGRFTQKFSHYEKVPDDIAAKVIAAWQES
                     ES"
     gene            2321..3073
                     /locus_tag="PK015_05640"
     CDS             2321..3073
                     /locus_tag="PK015_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOL33984.1"
                     /translation="MSGHNKWSSIKHKKGAADAKRGQLFTRLVKEIILAARNGGGDPE
                     ANPRLRTAILNAKAANMPRENIERAIKRGTGEIEGANYEEVVYEGYGPNGIGLVIEVM
                     TDNKNRTIADLRHTLTKYGGTMAESGAVAWNFEQKGFFNVPAAGLDEDEFMLQALEAG
                     AEDIELNGDYFDVYTAPQDFHTVLANMEKLGYPIENAELTRVPKTTINADDVAPKIFK
                     LIEMLEELDDVQKVYANFEVSDEVMEALSQEA"
     gene            3070..3546
                     /gene="ruvC"
                     /locus_tag="PK015_05645"
     CDS             3070..3546
                     /gene="ruvC"
                     /locus_tag="PK015_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOL33985.1"
                     /translation="MIIIGIDPGSRFCGYGLLEINERRILAAGCDVINLLKGKDLPQR
                     LEILFDAINDILREYKPDIAVVESIFYHKQIRSVFTLGQARGVILLALAQNDIPIVEY
                     SPREVKKAVVGNGNASKQQVRYMVNQIVNLHHKPAQDDAYDALGLALCHYHRIRFA"
     gene            3546..4151
                     /gene="ruvA"
                     /locus_tag="PK015_05650"
     CDS             3546..4151
                     /gene="ruvA"
                     /locus_tag="PK015_05650"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOL33986.1"
                     /translation="MLNYIHGILIHKNPMKAVVETASGIAFELMIPISTYEVLPETGK
                     DCLLYTHLQISQDDIRLYGFATQAECELYKHLNRISGVGPKIALSIISTLPIPTFVRA
                     IEKGEEALLTKIPGIGSKSAQRLIIELKGKLLHLLEYEVSEGKMLKEDIIMEVESALQ
                     ALGFNPKEVRRELSLLGDEAGKMPTEQLIKEIIRRIYQRSR"
     gene            4152..5465
                     /gene="rsmB"
                     /locus_tag="PK015_05655"
     CDS             4152..5465
                     /gene="rsmB"
                     /locus_tag="PK015_05655"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HOL33987.1"
                     /translation="MVREEAYYTILKVFKDTEFSDTLLHQRAKKLKGSKENVALFYNM
                     VKGVIKMRLKLDYILSQYTDKDKFAQTDIKIKTWLYLGLYQMMYLDSIPNHAAINETV
                     ELAKKNMGEKVGDFVNAILRAYQRNPEVKYPEDPVLRIAYEHSYPPELIAKWIELWGE
                     EDTEYLALFYNENPRLHIRVNTTATTRDKLIQYFTKRDIPVTPSPVSKITILTDAADE
                     VLEDVAFSEGYFSIQDTSSTLIVDLLDPHPEENILDLFAAPGGKCTYIAEKMVNTGEV
                     VAVDKIPNKMKLLKQAADRLQLTNIKQVVTDAFRYGPVAPAYDRVLADVPCSGWGVFG
                     RKADLRWQANKNIEELVKLQEKALDYAANFVRAGGILVYSTCTLNPAENEEQIKKFLQ
                     RNPKFTLVNAKEILPSDFVVEGMFKTLPFKHDMDGAFAAKMRKAR"
     gene            5472..6044
                     /locus_tag="PK015_05660"
     CDS             5472..6044
                     /locus_tag="PK015_05660"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOL33988.1"
                     /translation="MASEKTKKFWMTIGIGLGIIFITAFLTSQIIFPLIFRTPKNVEV
                     PNVVGLSLPMAKLRLTQLGLHIVVRDSVWSETGQPNTILDQDPQPGKKIKPESTVYVK
                     VNRGPMRVGVPYLVGLNYSDAYYTLKSAGLEAVIADSLYSDNYPRNTIVRSSPPQGTK
                     VEKGAKVKLYISKGPKPFIEETVIWDSLLM"
     gene            complement(6056..6826)
                     /locus_tag="PK015_05665"
     CDS             complement(6056..6826)
                     /locus_tag="PK015_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOL33989.1"
                     /translation="MSALIILLLLVIGACLGSFFNVLIDRIPRHQSIVKPGSHCTNCG
                     KPIPFSQNIPIISYIVLKGRCKACGAKIHWHHLVVEIITPVLFIALYLVYGLSFSFFK
                     FAIMTGFLIPIFFIDAFHKIIPLVISIPMVIIGLLMGLIQSEFNFYSFAMVFLLPVVI
                     LFVFLYLLALLWLKLFHREGLGGGDVILIPAVGAFFGVIHIPWVILLACILGLLYFLI
                     FVRKPNQGFAFGNIVALAAVVWTLIGDKFLHAIGWFNL"
     gene            complement(6833..7270)
                     /locus_tag="PK015_05670"
     CDS             complement(6833..7270)
                     /locus_tag="PK015_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33990.1"
                     /translation="MTKVFIILAALMMVLPVIAQVNMINPALFSNDTETISLLNPNRL
                     NMSHSMGFMAGTSSVGYGYYLSRYTNHLSYAFSQKLKMELDLNVINYGSTASSFKLND
                     DNNTRIVPDFRLSYQPSDKMHFQIEFHQGNYFPNYYNNWYDRW"
     gene            complement(7359..8027)
                     /gene="phoU"
                     /locus_tag="PK015_05675"
     CDS             complement(7359..8027)
                     /gene="phoU"
                     /locus_tag="PK015_05675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOL33991.1"
                     /translation="MLETRIQELKQMLISEAGLVEKMVSLSVDGLYKNIPSLLENVLA
                     LENQVNQTELEIDDKCITTIALYQPEARDLRLILMIYRINNDLERLADQAVNISESTA
                     HLMDNPVVYELPELEQMKSNTLTMLKNSLDALVKEDVAQSHQVCSEDNTVDNLNRQIY
                     HHLVELIQANPILTSQYLHLLRIAKNLERIADLSTNIAENTIYLAIGKVVRHRSEDIP
                     PISS"
     gene            complement(8037..8795)
                     /gene="pstB"
                     /locus_tag="PK015_05680"
     CDS             complement(8037..8795)
                     /gene="pstB"
                     /locus_tag="PK015_05680"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOL33992.1"
                     /translation="MVEKQIWTSNLNLWFGKNHILHNINLEAYKNNITAIIGPSGCGK
                     STLLRCFNRMNDLIPDMELTGKVYLGSKDIYAPKTDVYAVRASVGMVFQKPNPFPKSI
                     YHNVAYGLQIQAKYTKSEIKERVEESLKAAWLWDEVKDRLHHSAMSLSGGQQQRLCIA
                     RALANKPQVLLLDEPTSALDPQSTAKIEELCLSLKNSVTILMVTHNVAQAGRISDYTA
                     FLYLGELVEFGKTEHIFTVPKDKRTEEYLTGVFG"
     gene            complement(8789..9643)
                     /gene="pstA"
                     /locus_tag="PK015_05685"
     CDS             complement(8789..9643)
                     /gene="pstA"
                     /locus_tag="PK015_05685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOL33993.1"
                     /translation="MNLRKIKNDYWTGIVTLMLVITAGFLVLFIIRIVTMGASVLSWN
                     FIIQPPYKAMTAGGIFPALVGTFLLTILSMIIALPIGIFAAIWLTHYGKPARLVNFLH
                     IVINTMAGIPSIIFGLFGMAIFVNLMGFKVSLLSGSLTLAILALPVIINNTEQAIRSV
                     PADFSEASLALGATTRQTISRVVFPAALPNILTGAIISVGRVAGETAPIMFTAATFYS
                     RRLPHSLQDEVMALPYHIYALMTEGTHPEAQVPIAYGTALVLLILVLLIDATAIIIRS
                     YMRKKRQW"
     gene            complement(9640..10509)
                     /gene="pstC"
                     /locus_tag="PK015_05690"
     CDS             complement(9640..10509)
                     /gene="pstC"
                     /locus_tag="PK015_05690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOL33994.1"
                     /translation="MKDIKEKIFQTIAYLAALLTVICLLAIIYGIFHDGLSLFKVVSW
                     KDFLFTNNWYPTHYPPSFGAWALIIGSLTVTLGALIIALPLSLGSAIFIAEIASPRLR
                     EIVKPIIELLAGIPSVVYGLFGMAFLAPLIRQWFSLDTGLNLFTSSLILGIMVIPIIC
                     SISEDAMNSVPHSIREASLALGATKSETIFKAVIPAAKKGIIGSILLGFGRAIGETMV
                     VLMVSGGAAQIPKSIFEPVRPLTSTIAAEMGETVVGDLHYQSLFALAIVLFIITFVSN
                     LITELVFRPKRIK"
     gene            complement(10579..11388)
                     /locus_tag="PK015_05695"
     CDS             complement(10579..11388)
                     /locus_tag="PK015_05695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOL33995.1"
                     /translation="MKNKLLLLVAILTLTTITVNAKSKVTCAGSTTVLPIAQATAEAF
                     MDAHPDINISIRGGGSGVGIAALINNSVDIALSSRPMKSKEISQAKAKGINPVPYAIA
                     LDGIAVIVHPSNTISNITISQIRDIYTGKIKNWKELGGPNLPIVVISRDVSSGTFEVF
                     NEKVMAGAKVSSSAQLLASNNAVTNTVADTPGAIGYAGVGFVSNKVKPLAVEKVLPSE
                     KTVQDGTYILSRKLYMYTNGKATGAVSSYIAFIQSPQGQKIVKEQGFIPLQ"
     gene            complement(11399..12511)
                     /locus_tag="PK015_05700"
     CDS             complement(11399..12511)
                     /locus_tag="PK015_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL33996.1"
                     /translation="MKKLIWLTLLGLLLTGTLCSQELKISGEMWNRWTIVNGKVPADS
                     SNAILKNYFSLERGYFGLEPKFSETVKGRFTVDIFSSDDNKNGAGLKLKYAYLDFSNL
                     IPIPDLTTTVGLQKVYFGSIYDWTYDLIGKAPADEYSITNSSDYGITFNGYFPEGWGE
                     YAVGIYNGEGYKNYGANLKDNIEPAFVCNLRITPVSGIMVGGSIMINSNERKKLLTDN
                     TINPKFNQQLLLDGLGSITYGPINLCLEYISKKVMHADEFSNKDYTANCLSVIPTIKL
                     QKLIGYDLDLIGRYDRWDETDNPSNQNLLNAITAGLNYKFMHNESANPAMQFQLNYTY
                     KSYDEEESSASYANKLKDSSTLLLQLKWRFNNTITL"
     gene            complement(12600..13148)
                     /locus_tag="PK015_05705"
     CDS             complement(12600..13148)
                     /locus_tag="PK015_05705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOL33997.1"
                     /translation="MKSEENPIYDLDYDALLEEYSPLAYSICNQYKNAGLPLEDLQQE
                     ALLGLLEAANSYDATKGAKFSTYAVWHIKKRILSALKKEMAHSLQAETFEDNQITVNN
                     EEVHIKSSDWEKIIPSDMPPLEAKIIHLSYQEGLTLKEIAKNLDLTVERVKQIRNKAI
                     RRIKREQESLTSSISTNNNKLP"
     gene            complement(13138..14472)
                     /locus_tag="PK015_05710"
     CDS             complement(13138..14472)
                     /locus_tag="PK015_05710"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOL33998.1"
                     /translation="MRKNHLSTLLLIAVLLPLLSVGLCLILKASLGLTILVAAAVSLL
                     AIWFLCSSFRKIKDSWIHTARTVVQGDYSQRLQEFGIKEVDAVANEFNQMLERLEEVI
                     THLTIHRQELRLLLNSIEDVLWAQDDEGRIIWANEPFQKLFPAYNPAQKPHYQEIIRH
                     PELLAYLENRKTSPDKKIEEITIQNHSYILSYSLNQEAKRNIFILHNIDAIQKTEQMK
                     KDFIVNLAHELRTPMTAIQGFAEAMLTAPEQDNTRYLKIILNHSQRLNHLIGDLEQLI
                     QLESTAQLELQDINLSTFFDNIKLILEPEIQEKELNLEIELDPAIPRLVCDPFRLEQV
                     FINLVQNSLRYTDKGTISIKSRKEGNKVIFEVSDTGTGIAPEHLDRIFERFYVADPSR
                     TRSRNGTGLGLAIVKHIILLHHGDITVSSKLGEGTTFKITLPQKTLESGNEV"
     gene            complement(14469..15170)
                     /locus_tag="PK015_05715"
     CDS             complement(14469..15170)
                     /locus_tag="PK015_05715"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOL33999.1"
                     /translation="MNNKIFIVEDESDILELVKLILKNAGYQTEGFNGAKLMLNRLTE
                     QIPDLIILDLMLPDLDGMEACRIIKNRPEWETIPIIMLTARTDVEDRVRGLEYGADDY
                     ITKPFASSELVARVKAVLRRRGWETSKNVLTITSDFRIDFNKFEVYIRDKRVDLTLTE
                     FKILQLLTQRPGWVYSRNQILDYLWGNDKIVIERTVDVHIRNLREKLGDYAWTIKNIR
                     GMGYKFSPYEEDSGQ"
BASE COUNT         4830 a         2793 c         2973 g         4696 t
ORIGIN      
        1 aaaaaagggt ttgacaatat aacttgataa aaaacagagt tttcaaaata agattttatt
       61 ttacaaatct gtctaactct ttgaaaatca aagtaatgta gaaatagatg aggaacaaat
      121 gaaagaatat gaactgaaaa aaatgcgaaa tctgcttttt atgggtgcaa gcggttcagg
      181 aaagaccagt cttgcggagc aaatcttttt cctgacgcat actactaacc gtctgggtaa
      241 gattgatgaa ggtaatacgg tgatggattt tgacccggaa gagataagta aaaaaatgtc
      301 cttaggatta tcagtaggat atgctcatca taaagattgc aaaatcaata taatagatgt
      361 tccgggctct cctgatttta taggtgaaac catagtttct attcctgctg ttgaaaatgt
      421 agtgttagtg gttaatggaa cggcaggata tgaagttggc ttggaattag ctatagaaca
      481 gctggaaaat gagaaggcgg caaagatagt tctggtcaat agaatggata atgaacatgc
      541 agattttcaa aagacgctgg acactatata tgagaatgca gggttcaatc ctattcctgt
      601 gcatattcct attggtaaag aaagcacctt tgaaggagtg gtagatgtta ttcgtcagaa
      661 agccattctt cctggtggtg aaaaagatat tcctgcagat atgcaatcca ctattgctga
      721 agcccatcag aaattaatgg aagcaatagc agaaacggat gaagaattat taaatgattt
      781 tttagagaaa ggcgaactca gcgaagagca gctgattaat ggaatgcgca aagctctggc
      841 aaatggtgag atatgtgtag cttttgcctg ttctgcttca actggagttg gagttatggc
      901 atttttagat gccgtggaaa agtatttacc ttctccagag gatgttaatc ctatttcagt
      961 gctaagaaaa ggtgaacctg ctgagtttat tgcttctcca gatggagagc ttttaggcta
     1021 tgttttcaag ctatatgcag accccactat gggtgattat gcctatcttc gtctcttttc
     1081 aggttctctg aaaactggta tggatttttc tattcctgaa aaaggtgtaa aagagcgagc
     1141 aggcaatatc tattatatgt taggtaagaa tcgtgaagat gctcaagaac ttaaagctgg
     1201 agaaattggt gctttagtta agctgaaaaa tacccgagcc ttaaacacta ttactgctcc
     1261 cggttccgac ctttctttga agatgccgga attgcctact ccaacctact ggcaagcaat
     1321 taaagcattg aatcagagcg atgaagaaaa aattagtaat gccctgcaga aagtgattgc
     1381 agaagatcca actatccgct ttgaagttaa tcaagaaacc cacgagaata tcctttctgg
     1441 tctgggagaa cagcaattgc agctcgttct tcgcaaatta aagaatcgtt ataaagttga
     1501 tgctttcctt caagatcctc gtattcctta taaagaaacc attactacca gtggagaagc
     1561 tcaatatcgt cataagaaac agactggtgg aagaggacaa tatgccgagg tttacttccg
     1621 aatcaaacct cttgaacgag gtgaagggtt taagttcatc aattccattg tgggaggtgt
     1681 gattccctct aattttattc ctgctataga aaaaggtgta gtagagacga tggaaaaggg
     1741 tattatagca ggatatcccg tagttgatgt ctcagtggaa gtatattatg gcagttatca
     1801 tgaagtagat agttcagaaa tggctttcaa gattgctgct tctatgtgtt tcaaagaggg
     1861 atttaaaaaa tgcaaaccta tcttactgga acccattcat gagctgaccg taattgttcc
     1921 aactgaatat atgggtgatg tgatgggtga tataagcacg cgtagaggta gaattatggg
     1981 aatggaacag catggtaaaa aacaacacct aatagtccaa atgcccgtag ctgagatgta
     2041 ttcttactat cctgctttaa aatcgctcac tcaaggaaga ggtcgcttca ctcagaaatt
     2101 ctcacattat gagaaagttc ctgacgatat agcagcaaaa gtgattgctg cctggcaaga
     2161 atccgaaagc taatatttgc tataaaaatt gaacataaaa gaaaggggaa agccaggaaa
     2221 tgctttccct tttttcttac ttgacatttt atagccttag ggacaataag gttatgcatc
     2281 aatctttaat aaagataagt tagataaaaa agaggtttat atgtctggac acaataaatg
     2341 gagctctatc aagcataaaa aaggtgcagc tgatgccaaa agaggtcagc tttttactcg
     2401 cttagttaaa gaaattatcc ttgcggcaag aaatggagga ggagatcccg aagctaatcc
     2461 tcgtctacgt acagcaattt tgaatgctaa agcagcaaat atgccacgag aaaacattga
     2521 gagagcaatt aaacgaggaa ctggagaaat tgaaggggca aactatgaag aagtggtata
     2581 cgaaggttat ggacccaatg ggattggttt agttattgaa gttatgacgg ataataaaaa
     2641 tcgcactatt gctgatctcc gtcatacctt gactaaatat ggtggaacta tggcagaaag
     2701 tggtgctgta gcttggaatt ttgagcagaa aggctttttt aatgtgcctg cagctggatt
     2761 ggatgaggat gaatttatgc ttcaggctct ggaagcagga gctgaagata tagaattaaa
     2821 tggtgattat tttgatgttt acactgctcc tcaagatttt catactgttc ttgctaatat
     2881 ggaaaaatta ggctatccca ttgaaaatgc ggaattaaca cgtgtcccca aaaccactat
     2941 caatgctgat gatgttgcac ctaagatatt taaactgatt gaaatgctgg aagagctgga
     3001 tgatgtccag aaagtctatg ccaattttga agtctctgat gaagtaatgg aagctctcag
     3061 tcaagaagcg tgataattat tggtattgat cccggtagtc gtttttgtgg atatggtctt
     3121 ttagaaataa atgagagaag aattctcgca gctggttgtg atgtgattaa tttgctaaaa
     3181 ggtaaggact tacctcaaag attggaaatc ctttttgatg ctatcaacga tatcttaagg
     3241 gaatataaac ctgatatagc cgtggttgaa agtatatttt atcataaaca gatacgcagt
     3301 gttttcactc tgggacaggc tcgtggagtg attcttttgg ctctggctca aaatgatatt
     3361 cccatagttg aatacagtcc ccgggaagtt aaaaaagctg tggtaggtaa tggaaatgcg
     3421 agtaagcaac aggtgcgtta tatggttaat cagatagtta atctgcacca taaaccagca
     3481 caagacgatg cttatgatgc gttaggtctg gctttatgtc attatcatag gataagattt
     3541 gcctgatgct gaactatata catggaattt tgatacataa aaatccaatg aaagcagtag
     3601 tagagacagc ttccggtatc gcttttgaat taatgattcc aattagcacc tacgaagttc
     3661 ttcccgagac tggtaaagat tgtctgctgt acactcatct tcaaatctct caggatgata
     3721 tacgtcttta tggttttgcc actcaagctg aatgtgaact ttataaacat ttaaatcgta
     3781 tctctggagt aggaccaaag attgcacttt ccatcatttc tactttaccc attcccacct
     3841 tcgttagagc catagaaaag ggtgaagaag ctttactgac aaagattccc gggattggct
     3901 ctaagagtgc acaacgattg attattgaac tgaaaggtaa gctgcttcat cttctggaat
     3961 atgaagtttc cgaaggaaaa atgctgaaag aagatattat aatggaagta gaaagtgcac
     4021 ttcaggctct cggatttaat cccaaggaag tgcgtagaga gttaagtctg ttaggtgatg
     4081 aagcaggcaa gatgccaacc gaacaattaa taaaagaaat aatccgaaga atatatcaga
     4141 gaagtagata aatggttaga gaagaagcct attacactat ccttaaagta tttaaagata
     4201 cagaattttc cgataccctc ttgcatcaac gagctaaaaa gcttaaggga agcaaagaaa
     4261 atgtagccct cttctataat atggtgaaag gtgtgataaa gatgcgcctt aaactggatt
     4321 acatcctttc acaatatacg gataaagata aatttgctca gacggatatc aagattaaga
     4381 cctggttata tcttgggttg tatcagatga tgtatcttga ttctatacct aaccatgcag
     4441 caattaatga gacggttgag ctggcgaaaa agaatatggg tgagaaagtt ggagattttg
     4501 ttaatgctat tctaagagct tatcaacgca atccagaagt taaatatcca gaagatcccg
     4561 ttctacgcat agcttatgag cattcttatc ctccagaatt aattgctaaa tggattgaac
     4621 tctggggtga agaggatacg gaatatcttg ccctctttta caatgaaaat cccagattgc
     4681 acataagagt aaataccacc gccaccacca gagataaact catccagtat tttaccaaac
     4741 gtgatattcc tgtaacacct tctcccgtta gtaaaataac tatcttgact gatgctgcgg
     4801 atgaggtttt agaagatgtg gctttttcgg aaggatattt ttccattcag gacacctctt
     4861 ctaccttaat tgttgattta ttagaccctc atcctgagga aaatattctg gacctttttg
     4921 ctgctccagg tggtaaatgc acctatattg cggaaaaaat ggttaataca ggtgaagttg
     4981 tagctgtgga taagattccc aataaaatga aacttctcaa acaagcagca gaccgtttac
     5041 agcttaccaa tattaaacag gtggtcacgg atgcttttcg ttatggacct gtggcacctg
     5101 cttatgatag agttttagca gatgttcctt gttctggctg gggagttttt gggcgtaaag
     5161 cagatttgag atggcaagct aataaaaata ttgaggaact ggttaaactg caggaaaaag
     5221 ctcttgatta tgctgcaaat ttcgttcgtg ctggaggaat attggtctat tctacttgca
     5281 ctcttaatcc agcagaaaat gaagagcaga taaagaaatt tttgcagaga aatcctaagt
     5341 tcacattagt gaatgccaaa gaaatattac catctgattt tgtagtggaa ggaatgttta
     5401 agactttacc tttcaagcat gatatggatg gtgcttttgc tgctaagatg agaaaagcaa
     5461 ggtaagaagt tatggcaagt gaaaagacaa aaaaattctg gatgaccatc ggaattggtt
     5521 taggcattat ctttattact gcttttctga ccagtcaaat cattttcccg cttatcttcc
     5581 gtacccctaa aaatgttgaa gttcctaatg tggtcggact tagtttacca atggcaaagc
     5641 tccgacttac tcaactggga ttgcatattg tggtaaggga ttccgtatgg tcagaaacag
     5701 gacaaccaaa cactatttta gaccaggacc cccaacctgg aaaaaagata aaaccagaga
     5761 gcaccgtcta tgttaaagta aatagaggtc ctatgagggt gggtgtccct tatcttgtag
     5821 gattaaatta cagcgatgct tattataccc ttaaatctgc aggattagaa gcagtgatag
     5881 cagattctct atattcagat aattatcctc gcaataccat tgtgcgtagt tctcctccgc
     5941 aaggaactaa ggtagagaaa ggtgcgaagg ttaagttata tataagtaaa ggtcccaaac
     6001 cctttataga agagaccgtt atctgggata gtttattaat gtgatagtga atgtattata
     6061 aattaaacca accaatagcg tggagaaatt tgtctcctat cagtgtccat acaacagcag
     6121 ccagagcaac gatattacca aaggcaaaac cttgattggg cttacgaaca aagataagaa
     6181 aatatagtaa tcctaatata caggctaaaa gaataaccca ggggatgtga ataacgccaa
     6241 aaaatgctcc tactgctgga attaaaatta catcaccacc acctaatcct tcacgatgaa
     6301 ataactttaa ccacagcagg gcaagcaaat agagaaagac aaaaagaatc acaaccggga
     6361 gcagaaaaac catagcaaaa ctgtaaaaat tgaactcact ttggattaat cccatcaata
     6421 atcctatgat taccatagga atagatatta ccagcggaat gattttatgg aaagcatcaa
     6481 taaagaaaat aggaatgaga aaaccagtca taatagcaaa cttgaagaag ctaaaactca
     6541 atccatagac cagatacaga gcaataaaca atacgggagt aattatttct accaccaaat
     6601 gatgccaatg tatcttcgct ccacaagcct tacatcttcc cttcaggact atataactga
     6661 ttataggaat attctgcgaa aaggggatgg gttttccgca attggtgcaa tgagaaccag
     6721 gtttaactat ggattggtga cgaggaattc tatcaatcag gacattgaaa aagctaccta
     6781 agcaagcgcc aataaccaat aagagtaaga taatcaatgc actcaaagca gtttaccacc
     6841 tatcatacca gttattataa taattgggga aatagtttcc ctggtggaat tctatctgga
     6901 aatgcatctt atccgaaggt tgatagctca atctaaaatc aggaactatt ctggtattgt
     6961 tatcatcgtt aagcttaaaa gaagaagctg ttgaaccgta atttatcaca ttgaggtcca
     7021 gttccatttt caatttttga gaaaaagcat agcttaagtg attagtatag cgggaaagat
     7081 aatagccata tccaacagag gaagtgccag ccataaatcc catagaatga ctcatattaa
     7141 gtctgtttgg atttagcaac gaaatagttt ccgtatcatt ggaaaaaaga gctggattta
     7201 tcatatttac ttgtgctata acaggcaaga ccatcattaa ggcagcaaga attataaata
     7261 ctttcgtcat tttcccatct ccttctttat tctaaattcc actataatac aaaataaatt
     7321 aattcctttt cctgtcaaga aaagttgaac attggtcttt aactggagat aggtgggata
     7381 tcttcagagc gatgtctaac tactttacct atagcaagat agatggtatt ttcagcaata
     7441 ttggtggaga ggtctgctat cctttccaaa ttctttgcta tgcgtaataa atgcaaatat
     7501 tgggaagtca gaataggatt tgcttgtata agctccacca gatgatggta aatctggcga
     7561 ttcaggttat ccacagtatt atcttcgcta cagacttgat gagattgtgc cacatcttct
     7621 ttaaccaggg catccagact attttttagc atagttaaag tattgctttt catctgctcc
     7681 agttctggaa gctcataaac cacaggatta tccattagat gagcagtgct ttcagaaata
     7741 ttaaccgcct ggtcagccaa tctttccagg tcattattaa ttctataaat catcaggata
     7801 agtcttaaat ctctggcttc cggttgatat aaggcaatgg tagttatgca cttatcatca
     7861 atctccagtt cggtttgatt aacctggttt tccagtgcga ggacattttc cagcaaagaa
     7921 ggtatgttct tataaagacc atccacagat agagatacca tcttctctac caatcccgct
     7981 tctgatatca gcatttgttt taattcctgt attctcgttt ctaacatttt atctccttaa
     8041 ccgaacactc cggttaaata ttcttctgtc cttttatcct taggaaccgt gaaaatatgt
     8101 tcagtttttc caaattccac taattctcct aaatatagaa aggcggtgta atcagaaatt
     8161 cttcctgctt gtgctacatt atgggtaacc atcaggattg tcacgctatt tttaagagac
     8221 aggcataact cttctatctt tgcagtagat tgaggatcca gcgctgaagt aggttcatcc
     8281 agaagtaaaa cttggggttt atttgccaga gcccgagcaa tgcaaagtcg ttgttgttga
     8341 ccaccggata aactcatagc tgaatgatgt aaacggtctt tcacttcatc ccataaccaa
     8401 gcagctttaa gactttcttc cactctttct tttatctcgc ttttcgtata tttcgcctga
     8461 atctgaagtc catatgccac attatgatat atggatttag gaaaaggatt aggtttttga
     8521 aacaccattc ccacagaggc tctaacagca taaacatctg ttttaggagc atatatatct
     8581 ttacttccaa gatagacttt tccagtgagc tccatatcag ggataaggtc attcatacgg
     8641 ttaaaacatc gtagtaaagt ggattttcca cagccagaag gacctatgat ggcggtaata
     8701 ttatttttat aagcttccag attgatatta tgtaaaatat gatttttccc aaaccagagg
     8761 ttgagattgg aggtccagat ttgtttttct accattgtct tttcttcctc atataagaac
     8821 gtattattat agcggtggca tctattaata gaaccaagat aagaagcact aaagcagtcc
     8881 cataggcaat aggcacctgt gcttcaggat gagttccttc tgtcattaaa gcatagatat
     8941 gatagggtaa tgccataact tcatcttgca gactgtgtgg taatctgcga gagtagaagg
     9001 ttgctgcggt aaacataata ggagcagttt cacctgccac tcttcccaca ctgataattg
     9061 ctccagttaa gatattaggt aaggcagcag ggaagaccac tcgtgatata gtttgtctgg
     9121 ttgtcgctcc taaagctaaa gaagcttcag aaaaatctgc aggaacactt cgtattgctt
     9181 gctctgtatt attaataatc accggcaagg caagaatcgc cagagtcaaa cttccagata
     9241 aaagagatac cttaaaaccc attaagttaa cgaagatagc cattccaaac aagccaaaga
     9301 tgattgaagg tattcctgcc atagtattga tgacgatatg taaaaaatta actaaccgag
     9361 ctggttttcc ataatgagtt agccagatag cagcaaagat accgataggt aaggctataa
     9421 tcatagaaag tatagtaagt aaaaaagttc ctactaaagc aggaaagata cctccagccg
     9481 tcatagcttt ataaggaggt tgaattatga aattccagct taagacggat gcacccatag
     9541 tgacaatccg gatgataaaa aggactaaaa atcctgctgt aataactaac attaaagtaa
     9601 ctattcctgt ccaataatca ttttttattt tgcgtaagtt cattttatcc tcttaggtct
     9661 aaatactaat tccgtaatca ggttagatac aaaagttata ataaataata ctattgccag
     9721 agcgaacaga gattgataat gtaagtctcc aaccactgtt tctcccattt ctgctgctat
     9781 ggtagaagtc aatggacgca caggctcaaa gatagattta ggaatctgag ctgcacctcc
     9841 tgaaaccatt aagactacca ttgtttctcc tatagctctg ccaaaaccca ataaaatact
     9901 tcctattatc cctttttttg ctgctggtat tacagctttg aagatggtct cactttttgt
     9961 tgctcccagt gccagagaag cttctctaat actatgtggc acgctgttca ttgcatcttc
    10021 acttatgcta cagataatag gaatcaccat aataccaagt attaaagagg aagtgaagag
    10081 attcagacct gtatccagag aaaaccactg acgaattagg ggtgctaaaa atgccattcc
    10141 aaagagtcca tataccacgg aaggtattcc agccaataat tctattatcg gttttactat
    10201 ttctcttaag cgaggactgg ctatttctgc aataaagatg gcagaaccga ggcttaaagg
    10261 cagagcaatg attaaagctc ccagggtaac ggttaatgaa cctataatta aagcccaggc
    10321 tccaaaactt ggaggataat gcgtgggata ccaattattg gtaaatagga agtctttcca
    10381 gctaactacc ttaaaaagtg aaagtccatc gtgaaatatc ccataaatga ttgctaataa
    10441 gcatatgaca gttaataaag ctgctaaata agcgattgtc tgaaaaatct tttcttttat
    10501 gtctttcata ttttaacctt aaagacggga gtaataactc ccgtcataat ggaggattta
    10561 gggtttatag tcatagcttt attgcaaagg aataaaacct tgttctttaa ctatcttttg
    10621 accttgagga gattgaataa aagcaatata gctactgaca gctccagttg ctttgccatt
    10681 ggtatacata tacaatttcc gagataatat ataagtaccg tcttgcactg ttttctcgct
    10741 gggcaaaact ttttccacag ctaagggttt tactttattg cttacaaaac caactccagc
    10801 ataaccaata gctccaggtg tatctgctac agtattggtg actgcattat tagaagctaa
    10861 aagttgagca gaagaactga ccttagctcc agccataacc ttttcattga acacttcaaa
    10921 agttcccgat gaaacatcac gagagataac cacaatagga agattaggac cgcccagttc
    10981 tttccaattc tttatcttac cagtatagat atcccttatt tgagagatag tgatattgct
    11041 tatggtattg gagggatgga cgataacagc aattccatcc agagctatag catagggaac
    11101 agggttaatc cctttagcct tagcttggga gatttcttta gatttcatag gacgactgga
    11161 gagggcaata tccacagaat tattaatcaa agcagcgatt cctactccag aacctccacc
    11221 acgaatagag atattgatat ctggatgtgc atccataaaa gcttctgccg ttgcttgggc
    11281 tatgggaagg acagttgttg agcccgcaca ggttactttg cttttagcat taacagtaat
    11341 agttgtcagg gttaagattg ccacaagtaa taatagttta tttttcattt tttacatcct
    11401 atagtgttat tgtattatta aaacgccatt taagttgaag aagaagagta gagctatctt
    11461 ttagcttatt agcataggaa gctgaagatt cttcttcatc ataagatttg taagtataat
    11521 taagttgaaa ttgcatggct ggattagcac tttcattgtg cataaatttg taattcaagc
    11581 cagcagttat tgcattaagt aaattttgat tagagggatt atcagtttca tcccatctat
    11641 catatcttcc tattaaatct aaatcatatc ctataagctt ttgtaattta atggtaggga
    11701 taacacttag acaattagct gtatagtcct tattggagaa ttcatctgca tgcataactt
    11761 ttttagaaat gtattccaga cataagttta ttggaccata agtaatactg cccagtccat
    11821 ccaataatag ctgctgatta aactttggat taatcgtatt atctgtgaga agtttttttc
    11881 tttcattact atttatcata atagagccac caaccataat accacttacg ggggttatac
    11941 gtaagttaca cacaaaagcg ggttcaatat tatcctttaa attagctcca tagttcttat
    12001 atccttctcc attataaata cccactgcat attcacccca accttctgga aaatatccat
    12061 tgaaagtgat accgtaatcg gaagaattag ttatgctata ttcatccgca ggagcttttc
    12121 caataaggtc ataagtccaa tcatagatag aaccaaaata tactttctgt aatccaacgg
    12181 ttgtggttaa atccggtata gggataagat tggaaaaatc cagataagca tatttcaatt
    12241 ttaatccagc accattctta ttgtcatcag agctaaagat gtctacagta aagcggccct
    12301 ttacggtttc actgaatttg ggttccaatc cgaagtatcc cctttccaaa gaaaaatagt
    12361 ttttcaggat agcattacta ctgtctgctg gtacttttcc atttactata gtccagcgat
    12421 tccacatttc tccactaatt tttagttcct ggctacataa tgttcctgtc agaagtagcc
    12481 caagtagggt caaccaaatc aattttttca tatcttcttt ttcctccttt gaagatctac
    12541 tgtattatag gtatcaaatg ttaggatttg attagctgga ggttaagaat tgttaactat
    12601 taaggtaact tattattgtt agttgagata gaggaggtta gtgattcttg ctctcttttt
    12661 attcttcgga tggctttatt tcttatttgt ttcactcttt ccactgtgag gtctaagttc
    12721 ttagctatct ctttcagagt taaaccttct tgataggaca agtgaataat ctttgcttct
    12781 aagggtggca tatcggaagg aataattttt tcccagtcgg agctttttat atgcacttct
    12841 tcattattaa cggttatttg attatcttca aaggtttctg cctgtaaaga atgtgccatt
    12901 tcttttttaa gggcagaaag gattctcttt ttaatatgcc agactgcata ggtagaaaat
    12961 ttcgctcctt ttgtggcatc atagctattt gctgcttcta ataaccccaa taatgcttcc
    13021 tgctgcaaat cctccagagg aagacctgca tttttatatt gattgcagat gctatatgcc
    13081 agaggtgaat attcctccag caacgcatca tagtctaaat cataaatagg attctcttca
    13141 gacttcattg ccagattcta aggttttttg aggaagggta atcttgaaag tggttccttc
    13201 tcctaattta ctggagacgg taatatctcc atgatgtaat aggatgatgt gttttactat
    13261 tgccaatcct aaccctgtcc cattacgact tctggtgcga gaagggtctg ccacatagaa
    13321 acgttcaaag attctatcca gatgttcagg agctatgcct gttccggtat cagaaacttc
    13381 aaagatgacc ttattccctt cttttctact ttttatgctt attgttcctt tatccgtgta
    13441 gcgaagtgag ttttgcacta agtttataaa aacctgttcc aaacggaagg ggtcacacac
    13501 caatctcggg atagcagggt ccagttcaat ttccagattc aattctttct cctggatttc
    13561 aggttccaga atcaatttta tattgtcaaa gaaggtggaa agattaatgt cctgcaattc
    13621 cagttgagca gtactttcca gttggataag ttgttccagg tctccgataa gatgattcag
    13681 ccgctgactg tgatttaaga ttatcttcag atagcgagtg ttatcttgtt caggagcggt
    13741 taacattgct tccgcaaaac cttggattgc ggtcatagga gtcctcaatt cgtgagccag
    13801 attgacgata aagtccttct tcatctgttc cgttttttga atagcatcaa tattatggag
    13861 gataaatata ttgcgtttag cttcctgatt cagactatag gaaagaatat agctgtgatt
    13921 ctggatagtg atttcctcta ttttcttgtc tggtgaagtt ttcctatttt ccagataagc
    13981 taataattca ggatgacgga ttatttcctg ataatgaggt ttttgtgctg gattataggc
    14041 aggaaagagt ttctggaatg gttcgtttgc ccagattatt ctaccttcat catcctgagc
    14101 ccagagaaca tcctctatag aatttagcag taatctcagt tcctgtctat gaatggttaa
    14161 atgcgtaatc acttcttcca gtctttccaa catctgatta aattcattgg ctactgcatc
    14221 cacttccttt atgccaaact cttgtagacg ctgggaatag tctccttgaa caacggttct
    14281 ggctgtatgt atccagctat cttttatctt tcggaaagaa gagcagagaa accagatagc
    14341 aagaagtgag actgctgctg caactaagat ggttagccca agagatgctt taagaattaa
    14401 acataacccc acacttaaca agggaagaag gacggcaata agcaatagag tgctaagatg
    14461 atttttcctc attgtcctga atcttcctca tagggagaaa atttataacc cattccccgg
    14521 atatttttaa tcgtccaagc ataatcccct aatttttctc tcaaattgcg gatatggaca
    14581 tccacggtcc tttctatcac tatcttatca tttccccaga gataatccag aatctgattt
    14641 ctgctataga cccagccagg tctttgcgtg agaagttgca atatcttgaa ttcagttaag
    14701 gttaaatcta ccctcttatc ccttatataa acctcaaatt tattgaaatc tatgcgaaaa
    14761 tccgatgtga tggtaaggac atttttagag gtttcccagc ctcttctacg taacactgct
    14821 tttacccgag ctactaattc agaactggca aagggcttcg ttatatagtc atctgctcca
    14881 tattccagac ctctaactct atcttcaaca tctgttctgg cggttaacat tataatagga
    14941 attgtttccc attctggtct attcttaata attcggcaag cttccatccc atccaaatca
    15001 ggaagcatta agtccaggat aattaaatcg ggaatttgct cagttagacg attcagcatc
    15061 aactttgctc cattaaatcc ttccgtttga tatcctgcat tttttaatat caattttacc
    15121 aattccagaa tgtccgattc atcttctact ataaatatct tattattcat atcttatatc
    15181 ccctatttaa gactctcttc tctaaatatt attatctgac aaggaaaaac acttattttt
    15241 tgattaattt ttcttaacaa atggcagcat ggtggtgagt atggtggtga ga
//
LOCUS       DAORZF010000042        15176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_3142_length_15176_cov_348.038886, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000042 DAORZF010000000
VERSION     DAORZF010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 15176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15176
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3142_length_15176_cov_348.038886"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..4005
                     /locus_tag="PK015_05720"
     CDS             94..4005
                     /locus_tag="PK015_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOL34000.1"
                     /translation="MVEEMRKLWLALILLSLCVFTQAIIVETASLNNFLTGHEPACAY
                     DNWISHLAEGIAIPNYNLYAPYDRQTNGFGDFLLPTASDLVYWNDMLALFFVGDYNGA
                     QSILNNNNCPYQIVEFHDTDTGKTLYILREIPNMQYYDDNGTEDLYDDEAGAFVYGWG
                     IFIYYPEGSKPIIVTVPHPCDDFPTPRMGLQAFNVWDAQYLMINGAGREVRWTNQGTY
                     TNSKSLSDALRNPTHPFNYVYKKAADYLRDTFHCREWSCQIHSYDWNRHQGYANCQIS
                     AGNPRPCPNLPIRDLSPLKNDLINHGHHLMIPSNTVGIHNDVYLNDFYGVNYSIHDFI
                     FTDGEHTYPVNNQIDLPAYSENQQMLYSQAGTTDYDVFEPFFHIEMDELPNSYRESLH
                     NYYWFYGWNELLGKWDMDNLFTHFLEYYDRWIYDIEPVLEAMFQMDDGQPPVAPSNLA
                     VFNQSLNSVTLTWTKGSAFDFDSYEIYYATEPIDESNYQIFDRNNNAFLASPDCERIE
                     VTGLNNSYNYYFKIRARDKNQNYSGLSNEVTTSLAPVTISSFSAYGLDSTVRLYWQIG
                     AQQNNQGFKIYRKVENGEYVLVDSWLTNPALSNPSAYSFEWWDNDVMNRTRYTYKISS
                     TNVYNVEFFYNIPAIAEPRAIHTITISNSSGTLTDQISFSANPFATDGFDNYWDVTKS
                     TPTSNYVWNAFWEQYWGTNGTHLSREIKGDYDLDNELKTWVMRTRSDQINQTLTISVS
                     DNFDRAEKLYLYDGGNGAYHNLLSGPYQYLNSNSSIRTMTLFWGNMQPKVLITSMANK
                     IYQGGTTINFSWNYQYPFLVDHLELSIINDSGSILLSPSILNNQFSFTWTVPNDVTEM
                     QDCCFVVDAVAIDGVRTRFWSDYHFSLVPQMILSYNEPGLKMRSNPWLNSGLSFNQVF
                     GESQAYELSPEGLWIPTEDYDFGKAYCVFSDEVSFYSGTFPIQSNEYSLNLVPGWNFI
                     PNPHLCAYDLEDLSFLMRGELFNFSEVLAQNLISRAVYVYRNGIYEPVTRIEPWEAFF
                     IRYDGTEDLQPIIRFYPFFNGPSISPSEPGFKLKVVMNEGAPNSIELGLHKNATDDYD
                     FKYDLLKPFPLPYLETNSLWINLPNADSTDSWNLYSEYKAEFTQSKEQKFWNIRLEAV
                     SLEPITFNFEVQGTTPSWQIMMMLDDVSHIVGTSDNFIWTPPAPGIYEGYIRVSNYQV
                     GIDDFVQSPIKGLKIYPNPFNPEVNISFSTTTNNNVKINIYNIRGQKITTLYDGKLSA
                     GQHTFHWNGKDSNGRSAASGIYLLRVETGNEHHTLKMILMK"
     gene            complement(4073..5173)
                     /locus_tag="PK015_05725"
     CDS             complement(4073..5173)
                     /locus_tag="PK015_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34001.1"
                     /translation="MKKVLLILVPLLLVSLLAAIDFEFDGENRTRGALYNDVNEKDGG
                     HIDNRTLLGINALYRPNLSMRATLQFGDVVWGGSGGSIPASVPINAYELYLNYRFRNT
                     NFRIGQQYWKDNMSLILDDTFSGIMVSNDNLAGFETEVGWIKALERNRFDSDDYNYLL
                     LNIQKGTPIPWGLYASLGWDADYFNKDYTHITLMPYLSYDSGALTCNANVFMGLHFVE
                     DADTEVGIGAAVKADADVGPVVVSGDVLFATENGIAVLSPYYQNGLYIYGININNDAL
                     NLYWNNPYSWNNDTMLSLVGKVKGDITRNVSVFGAAGIVIDNGFEINGGVEFKLIPNV
                     LSLTGYAALGIGTDDGPTNYAIGTTLKMNLKD"
     gene            complement(5236..6324)
                     /locus_tag="PK015_05730"
     CDS             complement(5236..6324)
                     /locus_tag="PK015_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HOL34002.1"
                     /translation="MERLPVETLKNFMVEVFKRSGVPEEDAEICSEILIASDLRGIES
                     HGIGRLKMYYDRIKAGIQEPVTRIEVIKDKAATAVWDGNNGMGQVISFRAMETAIEKA
                     KQFGLGAVAVRNSTHFGICGYYANMACQENMIGLIFTNARPSVCPTFGVEPLLGTNPI
                     CFGAPTDLAFPFIYDAATSITQRGKIEQYAREEKETPVGWAIDLEGKPYTDTNQLLVD
                     LVKQQASLLPLGGTDELTGSHKGYGLATMVEILCAALQNGCFLDQLLGQDENGKAAPY
                     KLGHFFMAINIEFFTDLDSFKKTTGDICRRLQNSLTFPGQNRIWVAGEKEYEKEIEVR
                     REGIPVIPNLRKDIEVMQKELNIVPPVI"
     gene            complement(6343..7524)
                     /locus_tag="PK015_05735"
     CDS             complement(6343..7524)
                     /locus_tag="PK015_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HOL34003.1"
                     /translation="MYKEPYSFNLKRYMEPERFERFKEYAQTLDSPVLIIDLDIVEAK
                     YNELKDSFANLQIYYAVKANPMNEIIHLLDRLGANFDVASSYELRHLLNLGISPERIS
                     YGNTIKKAKDIRFFYEQGVQIFVTDSENDLKKIAENAPGSKVYFRLLTEGSGADWPLS
                     RKFGAHPDMIYSLILQAVELGLEPWGVSFHVGSQQRDIGQWDDAIARCKYIFDAALEE
                     GIELKMINLGGGYPANYVDPTFSFQEYSTEIQRFIFEDFGDNPPQLVMEPGRSLVADA
                     GIIVAEVVNIATKAKNNLYKWVYLDVGKFNGLIETIDESIKFPIYFEREGLADEVILA
                     GPTCDSMDIMYENFKYKMPDTTQPGDKVYIFTTGAYTQAYSSVNFNGFPPLKAVVLPK
                     K"
     gene            complement(7629..9362)
                     /locus_tag="PK015_05740"
     CDS             complement(7629..9362)
                     /locus_tag="PK015_05740"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOL34004.1"
                     /translation="MMSCKKISLILIFLSLFLSLFAQYNERDILTQQAFQLLGQRKFA
                     EAEQIFLQILDKYPNDSNSVIQLLNIYFQTSQMDKAENLLRQYRRILPANQATEQEIL
                     LLIMQGKPDEAKQKSDAYLLNQNYNQNICRLLASYFERRGFYEYSLQIYQEARRRKND
                     PELFRLEIANAALNASLFDLALSEYLAFLDKNPANIFFITNQCKIILNQNPECISTIS
                     DYAESSANPVIKELYANILVSQKQYLTALEVYKSLPQDRLVNFAEQQYNAGNDEIALL
                     AFQYLQSIISDPFIKNSYRLREALVHYRNGRYEATQETLNQILADSLMTERPYIYRQS
                     VNMNARKLMAETTLALEHNPQKAEYWYKEAKKFCANAYEIQNIDLALARLKLTQEDYQ
                     SALKILNSIQEPILRETRDYLIFNLELLQGNIEVADSLMNNFVIHFPQSPYVNDAVYQ
                     MMFVLGLDKTQQQQFFIATRLMMLQNPACVDTLLTIFENTQDEEILILAAEWSILLNQ
                     REKANTILEHSWQDPICNEYAELLKLSLTEDGEQMQRMAREFLKSNPGSIFAPKFRQY
                     ITRQELRPLQF"
     gene            complement(9359..12580)
                     /locus_tag="PK015_05745"
     CDS             complement(9359..12580)
                     /locus_tag="PK015_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34005.1"
                     /translation="MNEFLNKLQRFRFNLNLQIVLRCLIVATVFFMLGIHIYYLVWLN
                     VSAQSVVLIYLNYILRFGLIFLIIWIFYRGIKHFYRIGQTARWLDKQTEHQDDLYQNL
                     FELYQQKEDESILKVLASQATERLKSVSYRLPKLFPADLWFLILFLLIGIGSVWSFSA
                     DTFRYAMKQFAAITPETVAYKSTIDVIPGNITLGRGQQLVIQVVEPDTRLHHRLFYRW
                     DENWRELGLNNYSYTFPSLEYTLEYYVQNEVAKSPVYRVECLDEPFVKSWVIDYHYPA
                     YTGLGNVRDTLSYGNIEAFKHTEVILSISTNIPINKAIMHFSDGTTQTMQQVDANDFT
                     TRLKVLTPKTWYLELNDILGRSSEPEEKTISIIPDNPPEIRILYPGQDVMLDQSLQLP
                     LIISASDDFGLKNLSLHYQVNDHPAQSIILQSVINTKLFTTDYHFDMNNLNLLPGDNV
                     TYWAEVYDNSPDHQSAQTPKYKARFPSMEEIYKEIERLQNKQTQDLQSTLKEARNLQK
                     DFEKKRLELMKQDKISWEDAKQLEKMLNTQEKLTEQVENIAQDYQQLIDKLKANEALS
                     AETLQKMQRIQELMQEINTDELREAMSKVNEALRNLKPEDLRKAMENLKFSMEDFNKK
                     IDQTLQLLESIKKEQALDKALQISKEMEKMQSALMDKTQDPTQSNEQLAQEQKNIADK
                     LENLKEAMEQANQLLDPSKDKQIKQDLNDLLQEMNNGQLSQNLQQSQQQLQQNQRSAA
                     SQSQSQALEKMRQFTKRLSDMKNAMSGSSQQEVVNAMQKAIRELLIFSKQHESLKARY
                     SQDPYLIIPDLIAEYEGMQMSLNKLFSIPQVTMFIPPKFYMDLSDTDRSFKEIFNSVN
                     QMQYYQIPQQLENIQKGLNLMVYDLMQTLNNPSAGMGGGSGMQSLLQMLEQMGQEQMA
                     MNMLAEQLMMQLQQQGGRMGAAMQQQIQKLAAEEERLAENLKRALQNNPEAQKQGNAI
                     QQIIDEVEAVSRQLKNNQLTPEVLQSQERILSRLLDAQRSINKREFSEKRKGETANPE
                     IKQQAVETDYETLRRKAMLDDTYRLFPPAYQQVILKYLKLLNE"
     gene            complement(12679..13263)
                     /locus_tag="PK015_05750"
     CDS             complement(12679..13263)
                     /locus_tag="PK015_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOL34006.1"
                     /translation="MMLIDLIKRNRSYRRFDESKRISREELLEMVNAARLSSSARNLQ
                     PLRYRLVYKKEECDFVFSNLHFARSLQNWKGPQEGEHPAAYIILLLPKDAGNMQYIDT
                     GIAAQSITLSAVEKGYGCCNLGSVEKEELHYYFMFPEDRDIALVIALGVPIDQVVLEE
                     SRNPEDIQYWRDENNVHHTPKLKLEDIVLPEVHR"
     gene            13492..14256
                     /locus_tag="PK015_05755"
     CDS             13492..14256
                     /locus_tag="PK015_05755"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HOL34007.1"
                     /translation="MKRLNNKVSLITGAAKGMGEAEARLFAEEGASLIITDVDYTNLE
                     KVASDLQALGTKVLALKHNVASEEEWKKVAEEAEKTFGKVDVLVNNAGIFSVSGVEET
                     TMEIWNKVISVNQTGTWLGMKYIVPLMRKAGGGSIINISSVYALIGTGTSAAYQSTKG
                     AIRILSKTAAIEFAKDNIRVNTIFPGAIATTITETALDKPSKMEETTSDIIQIIPMKR
                     IGKPIEVAYGALYLASDESSYTTGAELVIDGGWTIP"
BASE COUNT         4630 a         2870 c         2668 g         5008 t
ORIGIN      
        1 aattggtttt caaaacagtg attgtgatat agcaaaaaat ccttgactaa taagttgaag
       61 tagataaatg aaaaatatgc aaaaatatca aaaatggtgg aagaaatgag aaagctatgg
      121 ttagctctta tattattatc tctatgcgtt ttcacacagg caataatagt agaaacagcg
      181 tccttaaata attttctaac tgggcatgaa cctgcttgtg cttatgataa ttggatttcc
      241 catttagcag aaggaattgc tattcctaat tacaatttat atgcacctta tgacagacag
      301 accaatggtt ttggagactt tctgctaccc acagccagtg atttggttta ctggaacgat
      361 atgcttgctt tattttttgt tggtgattat aatggtgctc aatccatttt aaataataat
      421 aattgtccct atcagatagt agaattccac gatactgata caggtaaaac tctttatata
      481 cttcgtgaga ttcccaatat gcaatattat gatgataatg ggacggaaga tctatatgat
      541 gatgaagcag gcgcttttgt gtatggttgg ggtattttta tctattatcc tgagggctca
      601 aaacctatta ttgtaaccgt tcctcatccc tgtgatgatt ttcccacccc gagaatggga
      661 cttcaggctt ttaatgtttg ggatgcacaa tatttaatga taaatggagc aggaagggag
      721 gttagatgga caaatcaagg aacttataca aattccaaat ctctttctga tgctttacgt
      781 aatcccactc atccctttaa ttatgtctac aaaaaggctg cagattattt aagggacaca
      841 tttcattgta gagaatggtc ctgccagatt cattcttatg attggaacag gcatcagggt
      901 tatgcaaatt gccagatttc tgcaggaaat ccacgtcctt gtccaaatct tccaattcgt
      961 gatttatctc ctttaaagaa tgatttgatt aatcatgggc atcatctaat gattccctca
     1021 aacactgtag gaatacataa tgatgtctat ctgaacgatt tttatggtgt gaattatagc
     1081 attcatgatt ttatttttac tgatggagag catacctacc cagttaataa tcagattgat
     1141 ttacctgcct attctgaaaa tcaacaaatg ctttattctc aagcgggaac cacagattat
     1201 gatgtttttg agcctttttt tcatatagaa atggatgagc tgcccaattc ttaccgggaa
     1261 tccttgcata attactattg gttttatggc tggaatgaac ttctgggaaa atgggatatg
     1321 gataatcttt ttacgcattt tttggaatat tatgatagat ggatttatga tatagaacct
     1381 gtcttagagg caatgtttca gatggatgat ggacagccac cagttgctcc ttccaattta
     1441 gcagtattta atcaatcttt aaacagtgtc actctcacct ggacaaaagg ctctgctttt
     1501 gattttgata gttatgaaat ttattatgcg acagaaccta tagatgaatc taactatcaa
     1561 atctttgatc gcaataataa tgctttttta gcttctccag actgtgaaag aatagaagtg
     1621 acaggattga ataattccta taattattat tttaaaatcc gagcacggga taaaaatcaa
     1681 aactactcag gcttatctaa tgaagtaacc acttccttag ctccagtcac aatttcatct
     1741 ttctcagcct atggattaga tagtactgtt cgtttatact ggcagattgg tgctcagcag
     1801 aacaatcaag gctttaagat atatcgtaaa gttgaaaatg gtgaatatgt tttagtagat
     1861 tcttggctaa ctaatccagc tttatccaat ccttctgctt attcttttga atggtgggat
     1921 aacgatgtta tgaatagaac tcgttatacc tataaaatta gctctaccaa tgtgtataat
     1981 gttgaatttt tctacaatat tccagctata gcagaaccga gagctattca tacgattacg
     2041 ataagtaata gtagtggcac actaacagac cagatatctt ttagtgctaa tccttttgcc
     2101 acggatggat ttgataatta ctgggatgtg accaaatcaa ctccaaccag taattatgtc
     2161 tggaacgctt tttgggaaca gtattggggc actaatggca ctcatctttc ccgcgaaatc
     2221 aaaggtgatt acgatctgga taatgaattg aagacttggg tgatgcgaac tcgttctgat
     2281 cagataaatc aaacattaac catcagtgtt tcagataatt ttgatcgtgc agaaaaacta
     2341 tatctctacg atggaggcaa tggcgcctac cataatctat taagtggacc ttatcaatat
     2401 ttaaattcta atagctctat tcgcactatg acattattct ggggtaatat gcaacctaaa
     2461 gtgcttatca cttctatggc taataagata tatcaaggtg ggaccaccat taattttagc
     2521 tggaattatc aatatccttt cctggtagac catcttgagc tttccattat taatgactcg
     2581 ggcagtatac ttctcagtcc atccattctc aataatcagt ttagtttcac ctggacagtt
     2641 cccaatgatg ttactgagat gcaggattgt tgttttgtcg tagatgcagt agctattgac
     2701 ggggtaagga cacgtttctg gtctgattat catttttctc tggtccctca aatgattctt
     2761 tcatataatg agcccgggtt gaagatgcgt tctaatcctt ggttaaattc aggtttaagt
     2821 tttaatcagg tttttggaga gtctcaagct tatgaattaa gtccggaagg tttatggatt
     2881 ccaaccgaag attacgattt cgggaaagcg tattgtgttt ttagtgatga agtatctttt
     2941 tatagcggaa ccttccctat tcaaagtaac gaatattcac tcaatttagt tcccggttgg
     3001 aattttattc ccaatccaca tctttgtgct tatgatcttg aagatttaag cttcctaatg
     3061 cgaggagaac tattcaactt tagtgaagta ctggcgcaaa atctgatttc tcgtgcagtt
     3121 tatgtctacc gtaatggaat ttacgaacct gtcacacgaa tagaaccctg ggaagctttc
     3181 tttattagat atgatggaac ggaagattta cagccaataa tcagattcta tcctttcttt
     3241 aatggacctt ctataagccc ttcggaacca ggattcaagc ttaaagtcgt aatgaatgag
     3301 ggtgcaccaa attccattga acttggatta cataaaaacg caacagatga ctatgatttt
     3361 aaatatgatc ttctgaaacc atttccctta ccttatctgg agacaaattc actctggata
     3421 aatcttccta atgctgatag tacggatagc tggaacttat atagtgagta taaagcagaa
     3481 ttcactcaat ctaaagaaca gaaattctgg aacatccgtt tggaagcagt ctctttggaa
     3541 ccaataacct tcaattttga agttcagggg actactcctt cttggcaaat tatgatgatg
     3601 ttagatgatg tttcccatat tgtaggtact tcagataact ttatctggac tcctccagct
     3661 ccggggattt atgaaggtta tataagggta agcaattatc aagtaggcat tgatgacttt
     3721 gtgcaaagtc ctataaaagg tctaaaaatc tatcctaatc cctttaatcc tgaggttaat
     3781 atttcttttt ccactactac taataataat gtaaaaatca atatatataa tattcgcgga
     3841 cagaagataa cgactttgta tgatggaaaa ttatctgctg gtcagcatac ttttcattgg
     3901 aatgggaagg atagcaatgg tcgttcagct gccagtggca tttatctgct cagagtggaa
     3961 actggaaatg aacatcatac tctaaagatg attttgatga aataatcttt ctttcagaaa
     4021 ataaaaaatc ccgggtcata agtcccggga tttttttatg atcaatgata gattaatctt
     4081 tgaggttcat ctttaaagtg gttccgatag catagttagt tggaccatca tctgttccaa
     4141 tgcccaaagc agcataaccg gttaaggaaa gaacattggg aataagctta aattctacac
     4201 caccgttaat ttcaaaacca ttatctatta ctattcctgc agcaccaaaa acggaaacat
     4261 tgcgggtaat atcacctttt accttaccta ctaaagaaag catggtgtca ttattccagc
     4321 tatagggatt gttccagtaa aggtttaagg catcgttgtt tatattaata ccataaatat
     4381 aaaggccatt ctggtaatat ggagaaagaa ctgcgatacc attttccgta gcaaataata
     4441 catctccgct aactacaaca ggaccaacat cagcatcagc tttaactgca gctccaatac
     4501 caacttctgt atctgcatcc tctacaaaat gaagtcccat aaacacattg gcattacaag
     4561 ttagtgcgcc tgaatcatag cttaaatagg gcatcaaggt gatatgagta taatctttgt
     4621 tgaaataatc agcatcccaa cccagagagg cataaagacc ccaaggtatg ggagtccctt
     4681 tttgtatatt aagcaataaa tagttataat cgtctgaatc aaatctgttt cgttctaagg
     4741 ctttaatcca tccaacttct gtttcaaaac ctgccaaatt gtcattacta accataatcc
     4801 ctgaaaaagt gtcatccaaa atcaagctca tattgtcttt ccagtattgt tgtccgattc
     4861 taaaattagt gtttctaaac cgataattga gataaagttc ataggcatta atcggaactg
     4921 aagctggtat ggaaccacct gaacctcccc atacgacatc gccaaattgt aaagtggctc
     4981 tcatagataa attaggacga tacaaggcat tgataccgag caaggtacgg ttgtctatat
     5041 gaccgccgtc cttttcattt acatcattat agagggcacc acgagtacga ttttctccat
     5101 caaattcaaa atcaattgcc gccaataaac tgaccagtaa gaggggaacg agtattaata
     5161 atactttttt cattgtagac tccttttttt gttatttatc aatatattaa tccttatcta
     5221 acgataaagc aaggattaaa taacaggagg aactatattc agttcttttt gcattacttc
     5281 tatatctttt cttaaatttg gaattacagg aatgccttct cttcttactt ctatctcttt
     5341 ttcatattct ttttcacctg ctacccagat acgattttga ccaggaaaag ttagagaatt
     5401 ttgcaatcta cgacagatat ctcccgtagt ctttttaaaa ctatccagat cagtaaaaaa
     5461 ctctatatta atagccataa agaaatgacc gagtttatag ggtgctgctt taccattttc
     5521 atcctgacct aataattggt ctaaaaaaca accattttga agagctgcac acaggatttc
     5581 taccattgta gcgagaccat atcctttatg gcttcccgta agttcatcag ttccacctaa
     5641 gggaagcaag gaagcttgtt gtttaactaa atcaaccaga agttgattgg tatctgtata
     5701 aggttttcct tccaaatcaa tagcccatcc taccggagtt tccttttctt ctctggcata
     5761 ttgttctatt ttccctcttt gcgtgataga ggttgcagca tcatagataa aaggaaaagc
     5821 taaatccgtg ggtgcaccaa aacagatagg attggttcct aaaaggggtt ctacaccaaa
     5881 agtaggacag accgagggac gagcattagt aaagatcaga ccaatcatat tttcctgaca
     5941 tgccatattg gcataatatc cacagatgcc aaaatgagtg gaattacgca cggctacagc
     6001 acctaatcca aattgctttg ctttttctat agcagtttcc attgctctaa aacttataac
     6061 ttgtcccata ccattattgc catcccagac agcggtagct gctttgtctt ttataacctc
     6121 aattctggta actggttcct gaatccctgc ttttatgcgg tcataataca tttttaaacg
     6181 accaataccg tgggattcaa ttcctcttaa gtcactggca ataaggattt cagagcatat
     6241 ttccgcatct tcttcaggaa cacctgagcg tttaaaaacc tcaaccataa agtttttcaa
     6301 ggtttcaact ggtaatcttt ccataattta gcctccagat atttactttt tgggtaaaac
     6361 tacagccttt agtggtggaa aaccattaaa atttaccgaa gaataggcct gtgtgtaagc
     6421 acctgtggtg aaaatgtaca ctttatctcc aggttgagtg gtatcaggca ttttgtattt
     6481 gaaattttca tacattatat ccatactatc gcaggtagga ccagcgagaa taacctcatc
     6541 tgccagtcct tctctttcaa agtagatagg gaatttgata gattcatcaa tagtttcaat
     6601 taaaccgtta aatttcccca catcaagata tacccattta taaagattat tttttgcttt
     6661 ggtggcaata ttaactactt ccgcaacgat gattccagca tcagccacca gagaccttcc
     6721 tggttccatt accagctgag gcggattgtc tccaaaatcc tcaaagataa agcgttgaat
     6781 ttcagtgcta tactcctgga aagagaaggt aggatcaaca taattagcag gatagccacc
     6841 tcccagatta atcattttta gttctattcc ttcttccagt gcagcatcaa agatatattt
     6901 acatctcgct atggcatcat cccattgtcc tatatctctt tgttgtgacc ccacatggaa
     6961 agaaactccc cagggctcta atccgagttc tactgcttgc agaataaggc tataaatcat
     7021 atctggatga gctccaaatt tacgagacag gggccaatca gcaccactac cttcagttaa
     7081 aagtctaaaa tatacctttg aacccggagc attttcagct atctttttta agtcattttc
     7141 actatctgta acaaaaatct gtactccttg ttcataaaag aaacggatat ctttcgcttt
     7201 cttgattgtg ttgccatagc ttatgcgttc aggtgaaata cctaaattca aaagatgtct
     7261 caattcatat gaagaagcca catcaaagtt tgccccaagt ctatccagta gatgtattat
     7321 ctcattcatt ggattggctt tcacagcata atatatctgc aaattggcaa agctatcttt
     7381 caattcatta tattttgctt caactatatc caagtcaatt atgagtaccg gactatccag
     7441 tgtttgagca tattctttaa aacgctcaaa ccgttccggt tccatatatc tttttaaatt
     7501 aaaggaatag ggttctttat acatcaaagc atccttagtt taatttgagt gagtccaaga
     7561 atcttagaaa caataatatg tcaattattc ctctcaatct cttttaaaat ctatatgtta
     7621 tttagctctt aaaattgaag aggccttagc tcttgacgag ttatatattg acggaattta
     7681 ggtgcaaaga tacttcctgg attggatttt aagaactctc gtgccattct ttgcatctgt
     7741 tccccatctt cagttaagct caattttaac agttctgcat attcattaca gatagggtct
     7801 tgccagctat gttctaaaat agtgttagcc ttttctcttt gattcaataa aatagaccat
     7861 tctgcggcta aaatgagtat ctcctcatcc tgagtgtttt caaaaatggt taataaagta
     7921 tccacacagg ctggattctg cagcatcata agacgagtag caataaagaa ttgctgttgt
     7981 tgagtcttat ccaaaccgag aacaaacatc atctgataga cagcatcatt gacataagga
     8041 ctttggggga agtggataac gaaattattc atcagactat cggctacctc tatatttcct
     8101 tgaagcaatt ccagattaaa gataaggtag tcacgagttt ctcgtaaaat aggctcctga
     8161 atagaattta gaattttcaa agcagattga taatcttcct gagtgagttt caaccgagcc
     8221 agagccaggt caatattttg aatctcataa gcgttagcac agaatttttt tgcttcctta
     8281 taccaatact ctgctttttg cggattatgt tccagagcta aggttgtttc agccatcaat
     8341 tttctcgcat tcatattcac actttgacga tagatataag gtcgttccgt catcaaagaa
     8401 tcagctaaaa tctgattaag ggtctcttga gttgcttcat atcttccatt tctgtagtga
     8461 accaaagcct ctcttaagcg ataggaattt ttgataaaag gatcagaaat aatgctttgc
     8521 aagtattgga aagcaagcaa cgcaatctca tcatttccag cattatattg ttgttccgca
     8581 aagttaacca gacggtcttg gggtaaggat ttatacactt ccaatgctgt taagtattgt
     8641 ttctgagaga ccaaaatatt ggcatagagt tctttaatta caggattagc actgctttca
     8701 gcataatctg atatagtgga tatacattct ggattctgat tcagaataat cttacactga
     8761 ttggtaataa aaaagatatt ggcaggattt ttatccagaa aagccagata ttcagacagg
     8821 gcaaggtcaa atagactggc atttagagca gcattagcaa tctccagacg gaaaagttca
     8881 gggtcatttt ttcttcttct tgcttcttga taaatttgca aggaatattc ataaaatcct
     8941 cttctttcaa aataggaagc aagtagacgg cagatatttt gattataatt ctggttgagt
     9001 aaataggcat cacttttctg ttttgcctca tctggtttac cttgcataat caagagtaaa
     9061 atttcttgtt cagtggcttg attagcaggt aatatccttc tatactgtcg taagagattt
     9121 tccgctttat ccatttgcga ggtctgaaaa tagatattga gtagttgaat cacgctattg
     9181 gaatcgttag gatatttatc cagaatctga agaaagatct gttctgcttc ggcaaatttt
     9241 ctctgtccca gaagctggaa ggcttgttga gtgagaatat ctcgttcatt gtattgagca
     9301 aataaagaaa ggaacaaact caggaaaatc aagattaaag atattttttt gcaagacatc
     9361 attcgttgag tagttttaaa tatttcagga taacttgttg ataggcaggc ggaaaaagtc
     9421 tataggtatc atccagcatt gctttacgcc gaagagtttc ataatctgtt tccactgcct
     9481 gttgtttaat ttctggatta gcagtttctc cttttctttt ctcggaaaat tctcgcttat
     9541 ttattgagcg ttgagcatcc aaaagacgag aaagaatgcg ttcctgactt tgaagaactt
     9601 caggggttag ttgattattt ttcagttgtc ggcttacagc ttccacttca tcaatgattt
     9661 gttgaatagc attgccctgt ttttgtgctt cgggattgtt ttgcaaagct cgcttaagat
     9721 tttcagctaa tctttcttct tcagctgcaa gtttttgtat ctgctgttgc atagctgccc
     9781 ccattctgcc accttgttgc tgtaattgca tcataagttg ttcggcaagc atattcatag
     9841 ccatctgttc ctgacccatt tgttccagca tctgaagcag agattgcatt ccagaaccac
     9901 cacccattcc agcagaagga ttatttaaag tctgcatcaa atcatagacc atcaggttca
     9961 agcccttttg aatattttcc agttgctgcg gaatctggta atactgcatc tgattcacac
    10021 tattaaagat ttccttaaaa ctcctatccg tatcacttaa atccatataa aatttgggag
    10081 gtataaacat tgtgacctga ggtatactaa aaagtttatt caaggacatc tgcatacctt
    10141 catattccgc tataaggtca ggaatgatga gataaggatc ctgactatat ctcgctttga
    10201 gagattcatg ctgtttggaa aagattaaca attctctaat ggctttttgc atagcattga
    10261 ccacttcctg ttgactgcta ccactcatag cattcttcat atcgcttaat cttttagtaa
    10321 attgtcgcat cttttccagg gcttgagatt gagattgact ggcagcggaa cgctgattct
    10381 gttgtaattg ctgctgactt tgctgcagat tttgacttaa ttgtccgtta ttcatttcct
    10441 gcaataagtc attcaaatcc tgctttatct gtttatcttt tgaaggatcc agcaattggt
    10501 tagcctgttc catagcttcc ttaagatttt ccaatttatc agcaatattt ttttgttcct
    10561 gcgcaagttg ttcattactt tgagtgggat cctgagtttt atccatcaag gcactttgca
    10621 tcttttccat ctctttagag atttgtaatg ctttatccag tgcctgctct ttttttatac
    10681 tttccaagag ctggagagtt tgatctatct ttttattgaa atcctccatg gagaatttaa
    10741 ggttttccat tgctttgcgg aggtcttccg gtttcagatt tcttaaagcc tcattaacct
    10801 tactcattgc ctctctaagc tcatcagtat ttatttcctg catcaattct tgtattcttt
    10861 gcattttctg gagagtctca gctgagaggg cttcattggc ttttagctta tctatcaatt
    10921 gttgatagtc ttgagcaatg ttttccacct gttcagtgag tttctcctga gtattaagca
    10981 tcttttccag ctgtttagca tcttcccagc ttattttatc ctgtttcatc aattccagtc
    11041 tttttttctc aaaatctttt tgcagatttc gtgcttcttt aagagtggat tgcaaatcct
    11101 gcgtttgttt attttgtaat cgttcaattt ctttataaat ctcttccata gagggaaagc
    11161 gtgctttata tttaggtgtt tgagcacttt gatggtccgg agaattatcg tatacttcag
    11221 cccaataggt cacattatcc ccaggcagga ggttcagatt attcatatca aaatgatagt
    11281 ctgtagtaaa gagtttagta ttaataaccg attgcaaaat aatagattga gccggatggt
    11341 cattaacctg atagtgaaga ctcaaatttt tcaatccaaa atcatcactc gctgatatga
    11401 ttaaaggaag ctgcaaactt tgatccagca taacatcctg tcctggataa aggatacgaa
    11461 tttcaggcgg attatcagga ataatggata tagtcttctc ttctggttct gaagagcgtc
    11521 ccagaatatc attaagttcc agataccagg ttttcggagt taaaactttt aatctggtag
    11581 taaaatcatt agcatcaacc tgctgcattg tttgcgtagt gccatcggag aaatgcatta
    11641 tggctttatt tataggaata ttggtagaaa tggaaagaat gacttccgta tgtttgaaag
    11701 cctctatatt gccataactc agggtatcac gaacatttcc taatcccgta taagcaggat
    11761 aatgataatc tatcacccag cttttaacaa aaggttcatc cagacattct accctataaa
    11821 caggactttt tgccacttca ttttgcacat aatattccag agtatattcc agagaaggga
    11881 aagtataaga atagttattc aatcctaatt ccctccaatt ttcatcccag cgataaaata
    11941 gcctgtgatg caagcgtgta tcaggctcta ctacctggat tactaattgc tgacctcttc
    12001 cgagagtgat attcccagga ataacatcaa tggtggattt ataggcaacc gtttccggtg
    12061 tgatggcagc aaattgcttc attgcatagc gaaaagtgtc agcagaaaag ctccaaacgc
    12121 ttcctattcc aattaaaaga aaaagtatga gaaaccataa atcagccgga aaaagtttcg
    12181 gtaaacgata agaaaccgat tttaaccttt ctgttgcctg tgatgcaagc acttttaata
    12241 tggattcatc ttctttttgc tgatagagtt caaaaagatt ttgatacagg tcatcctgat
    12301 gttctgtctg tttatccagc cagcgtgcag tttgccctat ccgataaaaa tgcttgatcc
    12361 cccgatagaa aatccagata attaaaaaga ttagcccaaa gcgtaaaata tagttcaaat
    12421 agattaaaac cacagattga gcagatacat tcaaccacac cagatagtag atatgaattc
    12481 ccagcataaa aaaaaccgta gccactatca aacaacgcaa aactatctgc agattgagat
    12541 tgaatctgaa cctctgaagt ttatttaaga attcattcat ctattttccc tgtattttac
    12601 taagcttttg cctaaacttt cctcttgggg taagatagct tttctatcgt aaaattatcc
    12661 cttagctcca tttctttttc agcgatgaac ctctggaagc actatatctt caagtttcag
    12721 tttaggagta tgatgcacat tattttcgtc tctccaatat tgtatatctt ctggatttct
    12781 ggattcttcc agcactacct ggtctatagg aactcctaaa gcaatcacca atgcaatatc
    12841 tctatcttca ggaaacataa aataataatg caattcctct ttttctactg aaccaagatt
    12901 acagcatcca tatccttttt ctaccgcact taaagttata ctctgagcgg caatacctgt
    12961 atctatatat tgcatattgc ctgcatcttt cggaagtaat agtattatat aggcagcggg
    13021 atgttcacct tcctggggac ctttccaatt ttgcaaagaa cgagcaaaat gcaaatttga
    13081 aaacacaaag tcacattcct cttttttata aactaaacgg tagcgcaaag gctgtaaatt
    13141 cctggcactg gaagaaagac gtgccgcatt taccatctcc aacaattcct ctctggatat
    13201 ccgtttgctt tcatcaaaac gccgatagct gcggtttctt ttgattaagt caattaacat
    13261 cattcctcct ttccttctat taacatattt ttatgcttta tactaagttt ttctaatttt
    13321 cctttctgtc aaacttaaat tccttctttt ccctttttgt ccaatttttc catcactttc
    13381 tgattatttt tcccccattt tttaaaaaaa taatttgcca agataaaaat cagaaattat
    13441 attatagatg atatcataca atataagtag gatattaaag gagaaaataa aatgaaaaga
    13501 cttaataaca aagtttccct aatcaccgga gcagcaaagg gtatgggaga agcagaagct
    13561 cgcctttttg ctgaagaagg tgcgtcttta attatcactg atgtagacta taccaatctg
    13621 gaaaaagttg cttccgacct tcaagctttg ggaacgaaag tgttggctct taaacataat
    13681 gtggcttctg aagaggagtg gaaaaaggtt gccgaagaag cggaaaaaac ttttgggaag
    13741 gtagatgtgc tggtgaataa cgctggtatt tttagtgtga gtggtgtgga agagaccact
    13801 atggagatat ggaataaagt gataagcgtt aatcaaactg gcacctggct gggtatgaag
    13861 tatattgtcc ccttaatgag aaaagcaggt ggaggttcta tcatcaatat ctcctcggtt
    13921 tacgctttaa taggaactgg aacctcagct gcttatcaat ctactaaagg agctatcaga
    13981 attttaagta aaactgctgc cattgaattt gcgaaggaca atatccgggt taataccata
    14041 ttcccaggtg ctattgccac tacaattacg gaaactgcac tggataagcc ttctaaaatg
    14101 gaagaaacta cttctgatat aattcagata atccctatga agcgaattgg aaaacctata
    14161 gaagtcgctt atggagcact ttatttagcc agtgatgaaa gctcttatac cactggagca
    14221 gagctggtta ttgatggtgg atggactatc ccataatctt tctttcgtca gataaagaag
    14281 aagaatgtaa gttcataaaa ttataagaaa ttaatgaaat caaaacctcc aagtatgttt
    14341 caggttgggg aaagattaag aaagtaatat cataaaatta tattgaatta aggactgaat
    14401 caggggatgc agaaaaattt agctgcatcc ccttcttttt tatatctatc tcaaatgcaa
    14461 tgtaatcttt tatttttcag gtagttcgta acgcataatt tctatcaagc gttaatcact
    14521 cgttaatcaa gtcttaaata gttaacgctt gattaacact tgattaagaa gtgataccaa
    14581 gagactatta agcaggaaat gaaataaatt attcaattaa ttgaaaagag gtttacttca
    14641 gctttcccat aaactccgat tgaataaagg aagaatgaag ataatagctg cgataatatc
    14701 tataacagtc cagatacctt ttttaaattt aaacataata atcggattaa atataagggc
    14761 agttaagata aacaaatata tgaagataga cctcatttta taaagtttaa agtgaagata
    14821 gatgagatag atggctgcaa taaatacaat aacccgcaga aaaatataaa aagaatattc
    14881 tatgtgaaaa atcccaccaa tgactaaaag taaagctatg agtggataga tatatatgct
    14941 ccatttcttt tccttctgac cgtcctcctt tctaaacttc tcaatattcc tgtctcctga
    15001 ttctaagtcc ttcatttccc ttgcctccta attaaatttc tttgcgatat ttataaaaga
    15061 aaaccctatc cctgtcaatt attatttatc ttttcaatat ttcctatgcc acattggaac
    15121 ttactggtgg aacgatgtcc tcatcgttta ttggtggaac aaggtccccc ttgttt
//
LOCUS       DAORZF010000043        14844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_3217_length_14844_cov_351.164717, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000043 DAORZF010000000
VERSION     DAORZF010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 14844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14844
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3217_length_14844_cov_351.164717"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="PK015_05760"
     CDS             complement(<1..1004)
                     /locus_tag="PK015_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOL34008.1"
                     /translation="MKKLFLLFIAFVNCFGLVTLSYANLISIGSGELRDIFFPVYSRE
                     AYSYSQQIYYQNEINYKGIITKLGFRYYSGNFNNTNHWRIYMGHTDKFEFNNTKDWIP
                     INNLIQVFDGDVSNYLSQGWLTITLTNPFEYNNNNNLVIAVAEVSELANDVNLYFNVY
                     RKMNLFNAGIYYYGNDYPNLSYPDSMTGKYAILDYTCQIRLEIISPPEKIILISPDNG
                     ENNVSITPTLSWDIPLTGDAPNGYKLFFRSEDEEEYTLYNQITTNYYHFVESLDYNTT
                     YYWKVLAYNNAGEGPISDEWHFVTIEDPPLYIEINSFFAFISNLNKVNLNWISQTETD
                     "
     gene            complement(1440..2819)
                     /locus_tag="PK015_05765"
     CDS             complement(1440..2819)
                     /locus_tag="PK015_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HOL34009.1"
                     /translation="MKKLGFDYPSSPRKTFLFDLEEQWYVPLLAEKRGLPIQPIKRSN
                     FGEYYMAVNYLTTVLEEAASINNLSIYNIDHMAQLRFTGKDAPALLDRALAGNMSAMK
                     VGSCKYTLLLNEHGGVEDDMIVMRISETEYIAVINAGHDITDTVNGKELIADIDRIMS
                     QKQNGEEVEAEDISAQLVKVDIQGPLSYKLVKSIYGEQVLKNRHNPEKNMNYFTFNEF
                     DYNNAHYIISRTGYTNRWGWELYIPVANAEEDFKKIVSEALEMGALLVGLGGRDENRL
                     SAGPFGLPLMGQEYDPYHTPINAPLFEAAVDMDKPFFVGKSALEAELATNPQKALLMF
                     FAEGIASNRKVFHNGKLLGTVTSSIISPNLSLEQRLVIGSTRKNVNAEDGNAAIGLAW
                     LYYNPFPKDEEGKYISEQDGVPIRIPVQFYRVDEQNNPLGTPLLGYLTMEGVSPATAP
                     KPLKYIENL"
     gene            complement(2869..4455)
                     /locus_tag="PK015_05770"
     CDS             complement(2869..4455)
                     /locus_tag="PK015_05770"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOL34010.1"
                     /translation="MNYIFTNARIFPLEQPGVSEFDSLLIESGKIKFLGSLSDCKIQS
                     KNHFEIINLQGKALLPAFTDCHTHFTDYAKQFIQLDLSGCRSINEVKQRLENYRETHP
                     KLTEWVLGGGWDINIIDNPQKIRKELLDEFFHDTPVALYSKDFHSRWCNSLALKIAGI
                     TENTSDPEGGHIERDNSGKLTGILQETASEWLDRFIIPPSKDETKQCLIQATEKVHSL
                     GLGTIHSMELPLSGEILEDFTRNYKKLRVIRHFYQDELQTMISQKRYSGEGDDWFHLG
                     GLKLFADGSLGSQTAAIFSEYPDSPDFHGILRLSEEEMYSLTLSALDYGFYTLIHCIG
                     DRAVHLAINALLRVKEEKPENHFKNRLEHIQSIREEDIPRLKACAAGCSVQPIHIAND
                     IPLIEKHWKTIEQEAYYFRSLIDKRIKLAFGSDAPIAPINPFYGIYSALQRKADLNPE
                     AKPWLPEQCLNIWEALQGYTINAAELAGIDNLAGTITPRKNADLMVLEDFTKLPDEYW
                     LEAESLLTMMGGEIVWNKLT"
     gene            complement(4436..5542)
                     /locus_tag="PK015_05775"
     CDS             complement(4436..5542)
                     /locus_tag="PK015_05775"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOL34011.1"
                     /translation="MIEAKRLHSVQISLIRQILQCAPSDAINLALGELQFPLPNLLRE
                     KSLEILQKGTPRYTPNAGMPELRETIAYHYPWAKPENVCVCNGVEEAIFVSLLSSLNP
                     GDKIAIPDPEYTAYSAIANILGAKVIRLPYESDLLSINWESWEKILAQNVKMLIFSHP
                     SNPCGHIFTKEEAERLITICNLNKITMVVDEVYSRLVFKNYMPEYFREAESIFLLDGL
                     SKSHCMSGWRIGWVISPPELSASVIKAKQYVSTCSNWLSQKLAVYALSAEGMKAVDEV
                     LAQLNECRFLVLDKFQNLADKVLIPDATCYMMLKVDGDDLIIAQELANKGVVTVPGQA
                     FGEVSKGWLRINYAVPIDKLEPALDTIINELYLH"
     gene            complement(5535..6359)
                     /locus_tag="PK015_05780"
     CDS             complement(5535..6359)
                     /locus_tag="PK015_05780"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HOL34012.1"
                     /translation="MNLKDAIIALYNAKPDKYSSADRELLETFIEALDRGEIRACEKT
                     DSGWQVNQWVKMGILIGFRMGALTEYLWSEKKSFFDKDTLPEKVFQLKDQVRIVPGGT
                     SIRKGSYIAKGVTIMPPAFVNIGAYVDSGTLIDSHALVGSCAQIGKNVHLSAGAMIGG
                     VLEPVEARPIVIEDDAFIGGNAGIYQGIIVQQRAVIAAGTIITASTLIYDAVLGEFLQ
                     RDSGGSFTIPERAVVVPGSRPLKDTTFQLYCPIIIKYRDAKTDESVKREEDLHIFD"
     gene            complement(6356..7522)
                     /locus_tag="PK015_05785"
     CDS             complement(6356..7522)
                     /locus_tag="PK015_05785"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HOL34013.1"
                     /translation="MKYDFDTVIDRRGSFCFKYDGLKEVYGREDLIPLWIADMDFQVA
                     PQIKAAMQKRLDHGIFGYNQRLPIYYETVINWAKKRYGYEPQEDWIMTSPGIMPAVNL
                     AVMQLTEPGDSVLIQTPVYQPFFSAVTAHNRKLVTNSLIPENGHYEIDFEDFEKKLKE
                     VKLFILCSPHNPVGRRWTDEELFRMGTLCRQYNVPIISDEIHGDLVYNGAGSRSIAAL
                     EGMLDNLIVCFSPAKSFNLAGLATAIIIAKNPALREPLDTMLENLHLYMGNTFGIIAL
                     TAAYRESEDWLEELICYLQGNRDFLVDYIQTELPQLKVNTPEATYLAWIDFSNLGLTD
                     DEVQKLLIEKAGLVLEPGIKYGIEGSGFQRLNFGCPRKTLSLALERMKKAIKEL"
     gene            complement(7512..8057)
                     /locus_tag="PK015_05790"
     CDS             complement(7512..8057)
                     /locus_tag="PK015_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34014.1"
                     /translation="MQRELNIQSVQGRMPVKFSVKTVNRILGILFQAFIIFCCLLVLF
                     TSLKKGSSVWLKIIPLIVLFVALDNLLRHLTTLNSLVFTPDYLWLKYLLLPSVQIPYD
                     NISKLELRKDITYNVFITYTDKKGKTRVLKCPASFPRMIEILYNIAELAPQAQMNDEL
                     AKMVNVIRDIKSKGEKVSDEV"
     gene            complement(8042..8899)
                     /locus_tag="PK015_05795"
     CDS             complement(8042..8899)
                     /locus_tag="PK015_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOL34015.1"
                     /translation="MIVKNINSIKHFNLHLHTNVSDGRLTPEEVVNRAQEIGLDLISI
                     TDHDNADAYNLLPQEIAPLRIIPGMEISSLYQDSDVHILGLGLNLHSQSLMDMIEMYL
                     VGRKERAIKMIQKLAELGMPINLDEVIAVAGSRGLIVRPHIAQVLLSHGYVHSKSEAF
                     EKYIGDNGPAYVHKPEFSVQEAIQVIHEADGFAIIAHPGDLANPAYVDDIIKMGIDGL
                     EVWHPDHNLSQIAYFTDLCKNLGLYMTGGSDFHGDNERYNLFEEVPASETILESVNKL
                     WREYKCRGN"
     gene            9007..9795
                     /locus_tag="PK015_05800"
     CDS             9007..9795
                     /locus_tag="PK015_05800"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOL34016.1"
                     /translation="MEYEPLKDRAANLIRIFPSLREKFYYLLDLLLLRQRYVKREINR
                     LFKPEDRFLYYDAGAGFCQYSWYVLKKYPSAKVFATDINGDYLKDFSEYASQVFPGRF
                     SWKRADLQTFIPDNKYHLVTAIDILEHIPDDIAVLQNFYKGMADKGILIISTPSDKDE
                     TAKFTEEHIRPGYNKKELEEKVQFCGFKILKSVYSYGFWGSLSWRLLIKLPLGMLSKS
                     KLWLTVLPFYYLLILPVAELMMQLDIHTYNSSGTGIILVAQKDV"
     gene            complement(9792..12257)
                     /gene="sppA"
                     /locus_tag="PK015_05805"
     CDS             complement(9792..12257)
                     /gene="sppA"
                     /locus_tag="PK015_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOL34017.1"
                     /translation="MKGRIWYIAFLLLLPGLLLADNNISWEAINLGEMDFPRASVDNL
                     FTPLVNPSLLGTGHSGGLGWAHTFSDEAFRNHYWFFANLGDFSYSYEYNRDPILDKSF
                     NYHTLATGGELFPRHIFPNLYGGLNYRWKNNKFDEGAFRTAFTYRPNALASLAFNWDN
                     PYKDSPAYHGSVALRPFAFVPSIKDYRLELSADIDYNKKDGEYKFNEPVIGLNTQIVD
                     GIKLGASYNLDTETAWLNFSLNIGNVSLGSTGIVKSDNYVAVPYIHLTEKSFPPFLGI
                     KDKKWYSMKLEGPIQTYKSPKFVINKLKIYESKIQSIEEVKDLLKQAKEDPAIEGILL
                     QNPASTGSFALQQEFTDAIKDFKESGKKVVFYYDNMSGGNYIFASSVADAIYLNPMGM
                     IDLRGISITSPYFKELLDNLGIDVLNFRSHKYKDAGNMFSEPEMTEAEREAYDSLLQS
                     IYDQFIATIQEGRGAKLKFSVEETIDNGPYFEAQDALNAGLIDGIIFADEINSVLKKQ
                     FGFNAQEKTLAEYVDYTWSYPKETPIAVIYACGNIVMGKGTPGTKIASQTTVDLIRKA
                     RQDPKYKGIILRVDSGGGSAQASDMILRELQLAQTVNKKPVVVSMSGTAASGGYYISC
                     GADRIIAEPSTITGSIGVVGLVFTFPRLYDKIGINWSTVKKGEHADFGATYRDWTDEE
                     KQLMTKTIESTYDRFVDKVDEGRKNLTREQVDAVAQGRVWTGTQAKENGLIDDLGGLD
                     KALEHMQELTKAKGKLVLEDATTSKGGFSMNMNFNLNGITTNNILSETMVNDYKALYE
                     WWKDYQNESVLMLCPLSLKDIEF"
     gene            complement(13115..13588)
                     /locus_tag="PK015_05810"
     CDS             complement(13115..13588)
                     /locus_tag="PK015_05810"
                     /inference="COORDINATES: protein motif:HMM:NF024954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOL34018.1"
                     /translation="MGRHKQSIDSITLEAIEQAISSIPNSSVVRKLTILSSYSRLKTE
                     EIAKAFRINPRTLFRWISQFKDQGVEGLVDKPKGRKPALLTDEMKRQLVQWIETKKDS
                     DGKEVSWTLDKLRAELEKVYSVKISKPALSVNLAKMNIRISELFPRISRQTRENR"
     gene            13968..14708
                     /locus_tag="PK015_05815"
     CDS             13968..14708
                     /locus_tag="PK015_05815"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOL34019.1"
                     /translation="MEIQEEFEKTGKWLFRWRSYLPLALIPLFLLAMEKYHYLNNSEA
                     SDNLWELLCLFISFLGLGIRVWTVGCTPPRTSGRNTRTQIADTLNTTGMYSIVRHPLY
                     LGNFFMLAGIVMFTRHLWLMLIYILLFALYYERIIFTEEAFLERKFGQDFIDWANKTP
                     AFIPKFKNYCPPANKFNWKKALKAEYNGFAALLLSMFALEVYGDWLIQHKIDLDLHWI
                     VLSGIGILTWITVRFLKKYTRVLDITKR"
BASE COUNT         4710 a         2845 c         2407 g         4882 t
ORIGIN      
        1 aaatctgttt ccgtctgact tatccagttc aaattgacct tatttaaatt agaaataaag
       61 gcaaagaatg agttaatttc tatatataga ggaggatcct ctatcgtaac aaagtgccac
      121 tcatctgaaa taggcccttc ccccgcatta ttataggcta atactttcca ataataagta
      181 gtattataat ctaaggattc aacgaaatga tagtagttag tagttatttg attatataag
      241 gtatattctt cttcatcttc acttctaaaa aacaacttat atccattagg tgcatcccct
      301 gttagtggaa tgtcccagct taaagtggga gtgatactta cattattttc tccattatca
      361 ggagatatta atattatctt ttcaggagga gatataattt caagacgaat ctgacaagta
      421 taatctaata tagcatattt tcctgtcata gaatcaggat aagaaagatt gggataatca
      481 ttaccataat aatatatccc tgcattaaat aaattcattt ttctataaac attaaaatat
      541 aagttaacat cgtttgctaa ttctgatacc tcagctacag caattactaa attattattg
      601 ttattatact caaagggatt agtaagagta atagttaacc atccctgtga taaataattt
      661 gatacatcac catcaaatac ttgtattaga ttattaattg gaatccaatc tttagtattg
      721 ttgaattcaa atttatcggt atgccccata taaatacgcc aatgattcgt attattgaaa
      781 ttaccagaat aatacctaaa acccaatttt gtaattatcc ccttataatt tatctcattt
      841 tgataatata tttgctgtga atacgaataa gcttcccttg aataaactgg aaagaaaata
      901 tcacgcagtt ctccgcttcc tattgaaatt aggtttgcat aacttaatgt aactaatcca
      961 aaacaattaa caaatgcaat aaacaataaa aataacttct tcataatcaa taccctttaa
     1021 ttttattttt tcacgatata ataaatatta tcattttctt gattataaat ctatctctat
     1081 tacatttatt gattatttat cttattgata ttaatgatta taaacacagt attaattttt
     1141 ttatagatac actaattatt taatcatata ttgtcaagaa aaagttaaaa ttgttaatgc
     1201 aatcaaaaat gatatattca taattcttta atatttcaag aagaaagcta ttcctggcat
     1261 aataacaggt tctgctttgg caaaagtgct tcagcttgaa agaatacttt aaactctatt
     1321 cttcttctgc ttcactcatt tgccagttct gagggaagaa gataatctaa aagtataaaa
     1381 aaatcagtct ggtacccgta atagatatcc agactggtag taaaaaaggc aggaatgtat
     1441 tatagatttt ctatatattt caaaggtttc ggagcagtag cagggctaac tccttccata
     1501 gtaagatatc ctaaaagagg tgttccaagc ggattatttt gttcatccac tctataaaat
     1561 tgtacaggta tacggatagg aactccatct tgttccgaga tatattttcc ttcttcgtct
     1621 ttagggaagg gattatagta taaccaggct aaaccaatgg cagcatttcc atcttcagca
     1681 ttaacatttt tgcgagtaga acctatgact aaacgctgtt ccagggaaag attgggtgat
     1741 attatactgg aggttacagt accgagtaat tttccattgt gaaagacttt acgattggat
     1801 gcaatacctt cggcaaagaa catcaaaagt gctttttgag gattagtcgc cagttctgct
     1861 tccagagctg attttcccac aaagaaaggt ttatccatat ctaccgcagc ttcaaataat
     1921 ggtgcattga taggtgtgtg atagggatca tattcctgtc ccatcagagg gagtccgaaa
     1981 ggtcctgcag aaagacgatt ctcatctcta ccacccaaac ctaccagaag agcacccatt
     2041 tccagagctt cacttactat tttcttgaaa tcttcctccg cattagccac gggaatataa
     2101 agctcccaac cccagcgatt agtgtaacct gttctactta taatataatg tgcattatta
     2161 tagtcaaatt cattgaaggt gaagtagttc atattcttct caggattatg cctatttttc
     2221 aagacttgtt caccatagat agacttaacc aatttatagg ataaaggtcc ctggatatca
     2281 acttttacta attgggctga tatatcctca gcctcaacct cttccccatt ttgcttctga
     2341 ctcattatgc ggtctatatc tgctatcaat tctttcccat taacggtatc agttatatca
     2401 tgtccagcgt taatcacggc aatatattct gtctcggaaa tccgcattac tatcatatca
     2461 tcttccactc ctccgtgttc attaagcaaa agggtatatt tacaggaacc gaccttcata
     2521 gcactcatat ttcctgctaa agctctatcc aacaaagctg gagcatcctt tcccgtgaaa
     2581 cgcaattgtg ccatatggtc aatgttataa atagataaat tgttaatgga agcagcttct
     2641 tccagaacgg tggtaagata gttaactgcc atataatatt cgccaaagtt actgcgttta
     2701 atcggttgaa ttggcaggcc tcttttctca gctaaaagag ggacatacca ttgctcttcc
     2761 aaatcaaaaa ggaaagtctt ccgaggtgaa gaaggataat caaaacctaa ctttttcatt
     2821 gttatctcct ataatatatt tatttaatac taaattattt cctataaatt aagtcaactt
     2881 attccagact atttcaccac ccatcatcgt caataaagat tctgcttcca gccagtattc
     2941 atctggaagt ttggtgaaat cttctaagac cataaggtct gcattttttc ttggagtaat
     3001 tgttcctgca agattatcta ttccggcaag ttcagcggca tttatagtat atccttgcaa
     3061 agcttcccat atatttaggc attgctcagg caaccagggt ttagcttctg gattcaaatc
     3121 cgcttttctt tgtagagcac tataaattcc ataaaaagga tttattggag caataggagc
     3181 atctgagcca aaggcaagtt ttattctttt atcaattaaa gagcgaaaat aataggcttc
     3241 ttgctcaatt gttttccagt gtttttctat taaagggata tcattggcaa tatgaatagg
     3301 ttgaacggaa caccctgctg cacaagcttt taaacgagga atatcttctt cccggataga
     3361 ttgaatatgc tccagacggt ttttaaaatg attttcaggt ttttcctctt ttacccttaa
     3421 aagtgcatta atagctaaat gcacggcacg gtctccaata cagtgaatca aagtataaaa
     3481 accatagtct aatgcagaca aggttaagct gtacatctct tcttcagaaa gtctcaagat
     3541 accatgaaaa tctggagaat caggatattc tgaaaagata gctgcggttt gagaaccaag
     3601 agaaccatcc gcaaataatt ttaatcctcc caaatgaaac caatcatcac cttcacctga
     3661 ataccttttc tgagaaatca tagtctgtag ttcatcctga taaaaatgac gaatcactct
     3721 gagtttttta tagttcctgg tgaaatcttc taaaatctcg cctgataaag gaagctccat
     3781 actgtgaata gtgcccaaac caaggctatg aaccttttcg gtagcttgaa tcagacactg
     3841 ctttgtctca tctttagaag gagggataat aaaacggtct aaccactcag aagcggtttc
     3901 ctgcaaaata ccagtcagct ttccagaatt atctctttca atatgaccac cttcaggatc
     3961 agaagtgttc tccgtaattc cagcaatttt tagagccaat gaattacacc agcgactatg
     4021 aaaatccttg ctatataaag caacaggcgt atcgtgaaaa aattcatcta aaagttcttt
     4081 tctaatcttt tggggattat ctataatatt tatgtcccag ccaccaccta aaacccattc
     4141 tgttaatttg ggatgagttt cccgataatt ttccagtctt tgcttgactt cattaatgct
     4201 cctacaaccg gataaatcca gctgaatgaa ttgctttgca taatcagtga aatgcgtgtg
     4261 acaatcagta aaagcaggaa gaagcgcttt gccttgtaaa tttataattt caaagtgatt
     4321 tttgctttgg attttgcagt cggataatga acccagaaac ttaatttttc ctgattctat
     4381 tagtaatgag tcaaactcac ttactccagg ttgttctaaa ggaaagatac gggcattagt
     4441 gaagatataa ttcattgata atagtgtcta aggcaggttc cagtttatct attggtactg
     4501 cataattaat tcttaaccaa cctttagaga cttctccaaa agcttgtccg gggactgtta
     4561 ccactccctt atttgccagt tcctgagcaa taatcaggtc atcaccatct acttttaaca
     4621 tcatataaca ggtggcatct ggaattaaga ctttatcagc aagattttga aatttgtcca
     4681 aaaccagaaa acgacattca ttaagctgag ccaaaacttc atccactgct ttcatacctt
     4741 ccgctgacag ggcatagaca gctaactttt gcgagagcca gttgctacaa gtagaaacat
     4801 attgcttggc ttttatcact gaagcagaaa gttctggagg ggaaatcacc caacctatcc
     4861 tccagccact catacaatgg gatttagaaa gtccatccag aagaaaaata gattctgcct
     4921 ccctaaagta ttcaggcata taatttttaa agactaatct tgaatatacc tcatccacta
     4981 ccatagttat tttatttaaa ttacagatgg ttattaatct ttctgcttct tctttagtga
     5041 atatatgacc acaaggatta ctgggatggc taaaaatgag cattttgaca ttctgagcca
     5101 gaatcttttc ccagctctcc cagtttatgc ttaataaatc actttcataa ggtaggcgta
     5161 taacctttgc tcccaagata ttagctattg cagaataagc ggtatattcg gggtcgggta
     5221 tagctatttt atcccctgga ttcaggctgg aaaggagaga gacaaaaatt gcttcttcca
     5281 ctccattaca tacacataca ttttctggtt ttgcccaagg atagtgataa gcaatagttt
     5341 ctcgtaattc tggcattcct gcattgggtg tgtagcgagg agttcctttc tgtaaaattt
     5401 ctaaactttt ctcacgtaaa agattgggta atgggaattg aagttcgccc agtgcaagat
     5461 ttatggcatc tgaaggagca cattgtaata tttgtctgat taaggagatt tgaacggaat
     5521 gtagcctttt tgcttcaatc aaagatatgt aaatcctctt ctcgttttac agattcgtct
     5581 gttttggcat ctctatattt aataataata gggcaataaa gctgaaaagt tgtgtcttta
     5641 agaggtctgc taccaggaac aactacagct cgctcaggaa tagtaaaaga tccaccagaa
     5701 tctctctgga gaaattcacc cagcactgca tcataaatca aagtggaagc agtgataata
     5761 gttccggctg cgattactgc tctttgctga acgattatac cctgataaat accagcattc
     5821 ccaccaataa aagcatcatc ttcaattact atgggtcttg cttccaccgg ttccagtaca
     5881 ccgccaatca tagctccagc agaaagatga acatttttcc ctatctgagc acaagaaccc
     5941 actaacgcat gagaatctat taaagttccc gaatctacat aagcaccaat attgacaaaa
     6001 gctggaggca taatagtcac accttttgcg atataacttc ccttacggat agatgtgccg
     6061 cctggaacta ttcttacttg gtctttaagc tgaaaaacct tttctggtaa ggtgtcttta
     6121 tcaaaaaagc ttttcttttc actccataga tattcagtta atgcacccat ccggaaaccg
     6181 ataagaattc ccatttttac ccattgatta acctgccatc ctgaatcagt tttttcacag
     6241 gctctgattt ctcctctatc taatgcttct atgaaagttt ccaacagttc tctatctgct
     6301 gaggaatatt tatccggttt tgcgttatat aatgctataa tggcatcttt taagttcata
     6361 gttctttaat tgcctttttc attctttcta aagcaaggga aagagtttta cgaggacaac
     6421 caaaattcaa tctttgaaag ccggaacctt ctatcccata tttaattcca ggttccagta
     6481 cgagtccggc tttttctatt aaaagctttt gcacttcatc atcagttaag cccagattgc
     6541 tgaagtctat ccaggcaaga taggttgctt ctggagtgtt gacttttaat tgagggagtt
     6601 cagtctggat gtaatcaacc agaaaatctc tattgccttg cagatagcaa ataagctctt
     6661 ccagccaatc ttccgattcc ctataagctg cagttaaagc tataatgcca aaagtattac
     6721 ccatatagag atgaagattt tctaacatag tatccagagg ctctcttaat gctggatttt
     6781 ttgcaatgat aatagcagtt gccagtcccg ccagattgaa agatttagca ggagaaaaac
     6841 aaacaataag attatccagc ataccttcca gtgcagctat agaacgagaa cctgctccat
     6901 tatagaccaa atcaccgtgt atttcatcac taatgatagg tacattatac tgacgacaaa
     6961 gggtgcccat tctaaaaagt tcttcgtctg tccatcttct tcctacagga ttatgaggac
     7021 tgcaaagaat aaagagtttc acttctttca atttcttctc aaaatcttca aaatctattt
     7081 catagtgccc gttttcagga attaacgaat tggttactaa ctttctatta tgtgccgtta
     7141 cagcagaaaa aaacggttga tatacaggag tttgaataag cacactatca cctggttcag
     7201 ttaactgcat aaccgccaga ttaacagcag gcataattcc agggctggtc ataatccaat
     7261 cttcctgagg ttcataacca tagcgtttct ttgcccaatt aataaccgtt tcataataaa
     7321 taggtaagcg ttgattgtaa ccaaaaatac catgatccaa tcttttctgc atagcagcct
     7381 taatttgagg tgccacttga aaatccatat ctgctatcca gagtggaatc aaatcttccc
     7441 tgccatagac ttcttttaag ccatcatatt taaaacagaa tgatcctctc cggtcaatta
     7501 ctgtatcaaa atcatacttc atcgctgacc ttctctcctt tagattttat gtccctgata
     7561 acattcacca ttttagccag ctcatcattc atttgtgctt gaggtgctaa ttcggcaatg
     7621 ttataaagta tttcaatcat cctgggaaaa gaagcaggac atttaagaac tcgtgttttc
     7681 ccttttttat cagtataagt aatgaataca ttataagtta tatctttccg caactccaat
     7741 ttggaaatgt tatcataagg aatttgcacg gaaggtagta ataaatattt tagccataga
     7801 taatcaggtg taaaaaccaa actattaagt gtagtcaaat ggcgtaaaag attatccaga
     7861 gctacgaaaa gcacaattag aggtataatt ttcaaccaga cagaagagcc ttttttcaga
     7921 gaagtaaaca ataccagtaa acaacaaaag ataataaaag cctgaaatag aatgcctaat
     7981 atcctattta cggtttttac actaaactta actggcatcc taccttgcac agattgaata
     8041 tttaattccc tctgcatttg tattctctcc aaagcttatt aacactttcc agaatggttt
     8101 cactggcagg aacttcctca aaaagattat atctttcatt atctccatga aaatcactgc
     8161 caccagtcat ataaagtccc agattcttac ataaatccgt gaaataagca atctgactta
     8221 aattatggtc aggatgccaa acttccaagc catcaattcc cattttaatg atatcatcca
     8281 cataagctgg attagctaaa tcaccaggat gagcaataat agcaaaacca tctgcttcat
     8341 gtatcacttg aatggcttcc tgaacagaaa attctggctt atgcacataa gcaggtccat
     8401 tatcaccaat atatttttca aaggcttcac ttttagaatg aacatagcca tgacttaata
     8461 gaacctgggc tatatgtgga cgaactataa gaccacgact tccagcaact gctatcactt
     8521 catctaaatt aataggcatc cccagttcag ccaatttctg aatcatcttg atagctcttt
     8581 cttttctacc aaccaagtac atttctatca tatccataag actttgtgaa tggagattaa
     8641 gtcctaaacc gaggatatga acatcagaat cctgataaag agaactaatc tccataccgg
     8701 gaattatcct taaaggtgca atctcttgcg gtaagaggtt gtaggcatct gcattatcat
     8761 gatcagtgat ggaaattaaa tccagtccta tctcttgagc tctattcact acttcttcag
     8821 gagtgagtct tccatcagat acattggtat gcaaatggag gttgaagtgc ttaatgctgt
     8881 ttatattttt tactatcata ttttctttta agttgactta attcttatgg cattaaaatc
     8941 gtcaatgatt ttttaattta atactttcag cttttttgat aaacagacac ataaaaagga
     9001 aatgttatgg aatatgaacc tttgaaagat agagcggcaa atcttatcag gattttcccc
     9061 tctcttagag aaaagtttta ttacctgtta gatctgcttt tgttgcgtca acgctatgta
     9121 aaaagagaaa taaaccgatt attcaaaccg gaagatagat ttctctacta cgatgcagga
     9181 gctggattct gtcagtattc ctggtatgtc ttgaaaaaat atccctctgc gaaagttttt
     9241 gctactgata tcaatggaga ttacctgaaa gatttttcag aatatgcttc tcaggttttt
     9301 cctggtcgtt tttcctggaa aagagcagac cttcagacat ttattcctga caataaatac
     9361 catttagtta ctgccataga cattttagaa cacattcctg atgatatagc tgtcctacag
     9421 aacttttata agggaatggc tgataagggg attctcataa tttccacccc ttcagataag
     9481 gatgaaactg caaaatttac tgaggaacat atacgaccag gatataataa aaaagaactg
     9541 gaagagaaag ttcagttttg tggctttaag attctgaaga gtgtttatag ttatggtttt
     9601 tggggaagcc tgtcctggcg tttgctgatc aaacttcccc ttggaatgct ttctaaaagt
     9661 aagctttggc taacagtttt accgttctat tatcttttaa tattgcctgt tgctgaactt
     9721 atgatgcaat tagatataca tacttacaat tcttctggca cgggtatcat actggttgcg
     9781 cagaaagatg tttaaaactc tatatcctta agagatagtg gacacagcat tagaactgat
     9841 tcattttgat aatctttcca ccattcatat aaagctttat agtcatttac cattgtttct
     9901 gaaaggatat tatttgtagt tataccatta agattaaaat tcatattcat agagaaacca
     9961 cctttagaag tggtagcatc ttccagaacc aatttacctt tagctttggt taattcttgc
    10021 atatgttcta aggctttatc cagaccacca aggtcatcaa tcaatccatt ttctttagct
    10081 tgagtgccag tccagactct accttgagct actgcatcta cctgttccct tgtaagattc
    10141 tttcttcctt catccacttt atctacaaaa cgatcatagg tggactcaat agttttggtc
    10201 atcaactgtt tttcttcatc agtccaatca cgataagtag ctccaaaatc ggcatgttca
    10261 ccttttttga ctgtagacca attgatacct attttatcat agagacgagg aaatgtaaaa
    10321 acgagaccta caactccaat agaaccagtt atagtggaag gttcagctat aatacgatca
    10381 gcaccacagg aaatatagta accaccagaa gcagcagttc cagacataga gaccaccact
    10441 ggttttttat tgacggtttg agcgagttgc aattcccgta aaatcatatc tgaagcttga
    10501 gcagaaccac caccactatc aactcttaga atgatacctt tatatttggg gtcctgacgt
    10561 gctttacgaa ttaaatctac cgtggtttga gatgctatct tagtgcccgg agttcctttt
    10621 cccatcacaa tattaccaca ggcataaata actgcgatag gagtttcttt aggatagctc
    10681 caggtgtaat ctacatattc agctaaagtt ttttcctgag cattaaagcc aaattgtttc
    10741 ttcaatacac tattaatttc gtcagcaaag atgataccat ctatcaatcc agcatttaga
    10801 gcatcttgag cttcaaaata aggtccatta tcaattgttt cttccactga aaacttaagt
    10861 ttggcacctc ttccttcctg aatggtagca ataaactgat cgtatatact ttgtagcaag
    10921 gaatcatatg cttctctttc tgcttctgtc atttctggtt cagaaaacat attccctgca
    10981 tctttatatt tatgactgcg gaaattgagc acatcaatac ccagattatc cagtaattcc
    11041 ttaaaatagg gactggtaat agaaattcct cttaaatcta tcatacccat aggatttaga
    11101 tagatagcgt ctgctacaga agaggcaaag atataatttc caccagacat attatcataa
    11161 taaaagacta ccttcttccc gctttcttta aaatctttaa ttgcatctgt aaattcttgt
    11221 tgcaatgcaa aggaaccagt agaagcggga ttctgcaaaa gtattccttc aattgcgggg
    11281 tcttcttttg cctgtttaag taaatctttt acttcttcta tactctggat tttgctttca
    11341 taaattttca acttatttat tacaaatttc ggtgatttat aggtttggat aggaccctca
    11401 agtttcatag aataccattt tttatccttg atgcctaaaa aaggaggaaa agatttttcc
    11461 gttagatgaa tatagggaac agctacataa ttatctgatt taactattcc agttgagcct
    11521 aaagatacat ttcctatatt caaactaaag tttaaccaag cggtctctgt atcaagatta
    11581 taggaagctc caagctttat accatctaca atttgagtgt ttaatccaat aactggctca
    11641 ttaaacttat attctccatc cttcttatta taatcaatat cagcagaaag ttctaagcga
    11701 taatctttga tggaaggaac aaaggcaaaa ggacgaagtg ctactgaacc atgataggca
    11761 ggagaatctt tataagggtt gtcccaattg aaggcaagag aagcaagagc atttggtcta
    11821 taggtgaaag cggtgcgaaa agctccttcg tcaaacttat tatttttcca ccgataattc
    11881 aaacctccat aaagattggg aaagatatga cggggaaaaa gttctcctcc tgtcgctaaa
    11941 gtatgataat tgaaactctt atccaggata gggtctctat tgtattcata actgtaactg
    12001 aaatctccca aattggcaaa aaaccagtaa tgattacgaa atgcttcatc actgaaagta
    12061 tgtgcccatc ctaaaccacc agaatgacca gttcctaata atgatggatt aactaagggt
    12121 gtgaatagat tgtctacact ggctcgggga aaatccatct ctccaagatt aatagcttcc
    12181 cagctgatgt tattatcagc taacagcaat ccaggtaaca gcaaaaggaa ggctatatac
    12241 caaattctac ctttcattct aacctcctca ggttttctat ttttaatgaa ttatttattt
    12301 aactatttag tagttattat tctttaacca tcaaaaattg actaaggctt ttgctgatac
    12361 atttaaataa ccataagtta aatgataata atttgtcaac tgttttgtct tagctttaaa
    12421 tagttagaat aaagacagtt gtatcagtaa ttaacttcaa ctatgaaaat tgacgacaga
    12481 agacaatgcc aaataaaaaa tctcatttca taataaaaaa aatagtgtat ccaaaaaagt
    12541 ataaagtata gttattatat ttgtgtaaga tctaagcata tcttttagat tttaaattct
    12601 aaccttttag ggtatgattc tcaagagctt taactataca cttctatgaa taagaattta
    12661 gaactcttgg atatatttct ttctgaattt ggttattacg gaaggagggg tgctatcagt
    12721 tttcctcttt ttaataactg gaaaaaaaca ttacctcaat taatacaaaa aaactcaggg
    12781 ttctaattgc attccaacta cttataatta tttgatgcag ttatgagatt aaagactatt
    12841 tcgtttataa catttacaaa gcactattat gattttctct tcttgtatat aatacaaagt
    12901 ttaatggtaa tgtcataaat atagataact gcaaagacac acaatagaag ttccggtaag
    12961 cttgtttgac ttttacctga agcagctttc ctgacatttc ccagacttgg gtcagactgg
    13021 tttagagacc aaagcgagct ttgtcaaaga ataatactct taggccttac tgcttttcag
    13081 cttttaactt caggtaattt agagtttttt tactctacct gttttccctt gtttgtcttg
    13141 atatgcgtgg gaagagttcg cttattcgta tattcatttt tgctagattt actgacaggg
    13201 caggtttact gatctttact gaatagactt tttcaagttc tgccctgagc ttgtccagcg
    13261 tccaagatac ctccttacca tcactatcct ttttcgtctc tatccactga actaattgtc
    13321 ttttcatctc atctgtcagg agagccggtt tacgcccttt aggtttgtct accagacctt
    13381 caactccttg atctttgaac tgacttatcc aacgaaatag agtgcgggga ttgattcgaa
    13441 aagccttcgc aatttcttcg gttttgagcc tggaataact agacagaatt gtcaactttc
    13501 tgaccacaga actgttaggt attgatgata tcgcctgctc tatagcttcc aatgtgattg
    13561 aatcaatgct ctgtttgtga cgtcccattt cttactcctt gtatgtaaag atataaacaa
    13621 gataatatga atttatggct tgtctatata aatcttgtat ctaacttaat taaaatactt
    13681 atttaaaaat ttttaaacaa ttttctatcc ttaattctat ttcataaaca gcattttctt
    13741 actttctacc ccataaaaat aatcttcagc tatataagca aaaacattaa ttaaagtaac
    13801 agaagatatc ataccatcag gaaattgggg taatacaaaa gttgtaaatt tctctggaca
    13861 gcataaaaat attctatatt atggttatat tgaatcttaa aaaagaaaag attcatttat
    13921 aagctgataa attagattat taatttcttt gcttgctgga gtttcttatg gaaatacaag
    13981 aagaatttga aaaaacaggg aaatggctat ttcgctggag aagttattta cctctggctt
    14041 taattccact tttcctttta gctatggaaa aatatcatta tttgaataat tctgaagcga
    14101 gtgacaatct ctgggaatta ttatgccttt tcattagttt tctgggatta ggaattcggg
    14161 tttggactgt aggttgtact cctccaagaa cttcaggaag gaataccaga actcagatag
    14221 cagatacttt aaatactacg ggaatgtatt ccattgttag acatcccctt tatctgggta
    14281 atttcttcat gctagcagga attgttatgt tcacacgtca tctatggtta atgctgattt
    14341 atatcctctt atttgcttta tattatgaga ggataatatt taccgaagaa gcttttctgg
    14401 aaaggaaatt tggacaagat ttcattgact gggctaataa aactcctgct tttataccta
    14461 aatttaaaaa ttattgccct cctgctaata agtttaactg gaaaaaagcc ttaaaagctg
    14521 aatataatgg ttttgccgca ttattactct ctatgtttgc tctggaagta tatggtgact
    14581 ggctaattca gcataaaata gatcttgatt tacactggat tgttttatcc ggtataggca
    14641 ttttaacctg gatcacggtc cgctttctta aaaaatatac cagagtgctg gatattacaa
    14701 aaagataaaa gaatactgac ttataaataa caactctctc tcacctcttt atctgttata
    14761 attgcccgat ttatcccaaa cttatgataa actgacgacc gatccggaca taagctggac
    14821 atgagttgga cataagctgg acat
//
LOCUS       DAORZF010000044        13262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_3662_length_13262_cov_387.423109, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000044 DAORZF010000000
VERSION     DAORZF010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 13262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13262
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3662_length_13262_cov_387.423109"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="PK015_05820"
     CDS             <1..721
                     /locus_tag="PK015_05820"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOL34020.1"
                     /translation="SNITRLYTYNEEGYAKRVDDISTNAETLYLQEGNWWEFDPIDPI
                     YQPQWLCYDLEAFHEQNLILYWQPPMADGIHQWTSYRIYDSYNQITPQLIAEVPFSQT
                     FWQRNITYTDHYILTVCAFDGNTESLPTNYVIVNPTAYNDPIATPPVLSIHPNPVCFS
                     VNQQVEIAIKSDVNLKGKINIYNIRGQVVKTIPLDNKGDFSYHWNGKDNKGTQCSSGI
                     YFVKVKIQGAPLLQKKLVLMK"
     gene            853..>1451
                     /locus_tag="PK015_05825"
     CDS             853..>1451
                     /locus_tag="PK015_05825"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOL34021.1"
                     /translation="MLYLPSRIFFGENALTESELYLIRLGTKPLIVTGKSSAKLSGAL
                     DELIPILKKAEQDYAIFDRVQENPSWDIVREGARFLKDAQCNYLIAIGGGSPIDAAKA
                     MALITANNLFEEENLTPDKFSQTLPLVAIPTTAGTGSEVTQYSVLTDTSTGIKAGFGT
                     ELAFPTLAICNPRYTVSLNATITLHTAIDALSHLLEGIFS"
     assembly_gap    1452..1551
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1552..1784
                     /locus_tag="PK015_05830"
     CDS             <1552..1784
                     /locus_tag="PK015_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HOL34022.1"
                     /translation="YYPYIKEELDNLFSNLNITREDFENWLDRFPLKAEFDLSEEFIE
                     SHIAEVLASRNMANTPIKVKEEDIRKIYKSLK"
     gene            complement(1792..2652)
                     /locus_tag="PK015_05835"
     CDS             complement(1792..2652)
                     /locus_tag="PK015_05835"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF005491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HOL34023.1"
                     /translation="MGNKCKRILAIHDFSGFGHTSLMVCIPIMYRMGIRVCALPSAYL
                     SANTDYPEPIWIDMSSYLDKINAHWQEIGLSFDAISSGFLASPAQVVQIGKVIESWRE
                     KVKLVLIDPVMGDYGKLYSCYDDNMIVAMRELISLADVITPNYSEAAWLAKGVIRKIG
                     EENELLDWCRKISEYGPQHIVITSVPGSVSDSLKVIYYQAKLDKLSTYPFVKKDGIYP
                     GAGDCFSALLLSGLVNDYDFDASIKATIKIISRAMEEPIMEGTDWREGLPLEKIIQWD
                     LRSIFSETDA"
     gene            complement(2955..4502)
                     /locus_tag="PK015_05840"
     CDS             complement(2955..4502)
                     /locus_tag="PK015_05840"
                     /inference="COORDINATES: protein motif:HMM:NF036720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAVED domain-containing protein"
                     /protein_id="HOL34024.1"
                     /translation="MTFPELVSAYLNEGNDLLKNKIVDYLNSNNFSEEDWSSIIHLLF
                     NPYSNTVSALAWLALIANSHQDEELAKSLNLNPGQFSELFQSRLRKASFPVVDQQSNG
                     VLAEVLIFPFSSTESRAICFNKIYAEQANMLAQLTGRSFLMVFTEDFVGDSWMAATAA
                     ALIADNPDELRDFCFTGAVNESGKIMPPAQLAEKKKCCEARGKKLITSVKSLEELEFW
                     LNSSELPVPVVQSSRTPDKKSSWLLKMEAAIQKEYPLFNLQALFEFTGLNEDDLSIFK
                     EGDFPFIPEVWQTFLGSIDHFDRVEQKLSSRKALFWYAGMLSSLQFGIGAIFGFNRPI
                     YICHWEPTNQEYLPVFRLYRPNNARILKNVSFDPHSFTHIKYEINAPSCQMKDLALIL
                     YFGSHNPYQEVISYFKPLFPDSGTLFVYLKESQGQINLDSDWLRIVQETNSLLNYLKN
                     SYHWQRLHIFQSSPNAICMALGIALGHFTPATIYHYQPQAEREHYKGMYDLEMVKNYR
                     NQFASQT"
     gene            complement(4499..5434)
                     /locus_tag="PK015_05845"
     CDS             complement(4499..5434)
                     /locus_tag="PK015_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34025.1"
                     /translation="MKNEEFEKELFEIYLDSLEDRFDFDEDKEKIESLTTEAGEANII
                     VEQNNIEPIETQVLGTIRQLSHSGKLINYVPVALQENGLYLVMKVSPFIHYALDTDII
                     APGYSGQYLIDPYNNFLLYPEEIEDSVVLDYLDLMTIFELQEALEKSIKEKQEGKGID
                     YSAIQDKWKLEFRFKEYQLTSYFRFRDLDANALDLTPMVDLLVKKEGLAMNKYAAARP
                     TLTQAFDLMRQPETSNIFYALTHTLVKEPDGGILLYPDEDYIGKLGKIYICTECVFEG
                     YIPKQLWLGLRGFKDLKSIQEWLNISIEIEEEEKK"
     gene            complement(5431..6252)
                     /locus_tag="PK015_05850"
     CDS             complement(5431..6252)
                     /locus_tag="PK015_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34026.1"
                     /translation="MLYSCDQNKYQSWYNSLSLEERSWQKIIAKLWQIFQLYPEKELS
                     DTDIFYLISLIPSLQKKQEKLIYDLCSKRNISYWVTRVSRAGDFLYLICPQLDSLKKN
                     LEGKNGEQLYRYLTRVFQNIIIQCNNKIIKDLESAESNYDDNLMNSSCKEIYHPAKIK
                     TMGEIILEEENRRGAEKLYIQIKQEWSLNKINLLCEYYDKVNGIKTERLKAESENNIY
                     KLHQRLRNDFNELLTQYTKDEIRTFFKLYLPELCQETPPSPTYLYNKKTKAEELL"
     gene            complement(6478..8217)
                     /gene="rpoD"
                     /locus_tag="PK015_05855"
     CDS             complement(6478..8217)
                     /gene="rpoD"
                     /locus_tag="PK015_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HOL34027.1"
                     /translation="MITYNECLKKLVELGKDTGYITFKQINDILPNSPFFLDKVDDII
                     FDLGQEGIEIIDETEKRLTKGGVAIKKPNVPKKFKNKRYYEDPVRMYLKEMGRVPLLD
                     REGEVRVAQKIETYQKMINQNVFKSGSTLREMYNFLHRYREHRVRLDQIFHIDLGTWI
                     DKKQDAVIIEQFKEILKKNEATFNKIGEILDSCDYDESGNATRQEEIEALRNDIVNTF
                     NQLSYNEILIKRMVYRIRSLVERIEESYATINELTRIERYTIDEICTYGRRAKKSPED
                     FEEVRKETGIDPEIFIETLRKIKNARRKIRRVELETKMTGSDLIFLLREIERAERNKE
                     RAQHEMIEANVRLVISIAKHYNNRGLEFLDLIQEGNTGLMRAVEKYDYRKGYKFSTYA
                     TWWIRQAITRAIADQARTIRIPVHMIESINKINRTTRRLQQKLGRDPYPEEIAEVLDM
                     PVEKVKSVLSIAKEPVSLDKPISNDNEDSVLSDFIPDRTTISPERLAERTMLKKQVDE
                     VLKTLTTREERVIRLRFGIDDGYHRTLEEVGKIFQVTRERVRQIEEKALKKLRHPSRA
                     AVLQQFLDNFNVK"
     gene            complement(8244..9956)
                     /gene="dnaG"
                     /locus_tag="PK015_05860"
     CDS             complement(8244..9956)
                     /gene="dnaG"
                     /locus_tag="PK015_05860"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOL34028.1"
                     /translation="MDKNLIEEIRRSNDIVDVVQEYVPLKKVGTNWRGICPFHNDTHP
                     SLYVSQPKQIFKCFACGKAGNVFTFVQEYEKMSFYEAVKKLAQRAGIVIPEKERISPA
                     STKRAQLLRIYTETRDFFTNQLMKQKEVLSYLQNRAISPETAKTLELGYAPEGEKILL
                     NYLLDKGYSVDLLKDSGLFGNYSGNLVDFFRNRLMFPIHNSIGEVVAFGGRILEENEN
                     VGKYVNTPGTELYTKGKELYGFFKTKYEISKIGYALICEGYFDFLRLYENGFQNAVAS
                     LGTSLTEDQIYLLARYTNKVYMLYDGDAAGIKSAQRAALLCLSKGLDASIIELPEKDD
                     PDSFLLENGPEALQERIAAAKDVIHFMAEAKSKTPLMERIDQLLDAVRSIKDPIKREL
                     IVKDISEAFNVSTRSLFSKLHRTSVVETEGLYSTPALEEYPEERQLLILALKDPDNFN
                     LLAQDLSPDYFNNKRYKELFKYLVNNASANMISEPAQLLDLMENQELRGLVGDFLFEE
                     MQDLDFMSVLEQVKIRKIQRDLRDIDRRIMAEPENKTLLKEKSKLVALYRQMTSKVVH
                     KDLT"
     gene            complement(9959..12307)
                     /locus_tag="PK015_05865"
     CDS             complement(9959..12307)
                     /locus_tag="PK015_05865"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HOL34029.1"
                     /translation="MNDKELYADLEYSRLVEEVYTRCESILGKERAKQLRPLSEKKEI
                     EASLELVSELQEALNRGISINFTNLSNLQPLFAEATYSLFGFEEFQQVYLQVSLAEAV
                     SEKAESVEDFPVLKKLWQKVKPLNEIKTRFEKIFAPEGEVLDTASVELNRIRKRFASL
                     QRSIQKTMQNMLNDARYENFLQDKFITRREGRYVLPVKGNAGAFVPGIVQGQSGSGST
                     LFIEPESVVPLNNELQLLLQEEKREIFNIFNEFTAQIKEQKDILIANQEALTELDFRF
                     GCARLCYTLKAKKPQIVDEPVLKLYSARHPLLILSLNDVKKVVPFDLNLGETARIILL
                     SGPNTGGKTVLLKATGLLTLMALSGLPIPAEENSVIGRFNQVFADIGDEQSIENALST
                     FSSHIGKIGRMLKSANEKSLVLIDEIGAATDPQQGSALAQAILERLTELGSKAVVTTH
                     YTALKIFAETHPDCLNASMQFDLKSLTPTYRFTTGIPGDSFAIEVASSLGLEPDLIER
                     AKELSGSQNIEFTELLKKMQEEKKALARATYEYELKTRNLESKLKELESREAALNAEL
                     KERRKKYLQELQSELIAQQKLYQREQESLKKMEREERKSVSERKLQDINNRLQDINKE
                     LSDSEIEQRQKIRVPREGDRVWLATLETEVTILEIKDQQAIVDLNGITFKTALDNLYE
                     IGKPAKKEEMVVITHSNPPPKAQFELKLLGLTFEEAKPLIDEFLDDAMLAGLHTLRIV
                     HGKGTGALRTKVRDYLKKKKQVISLETPLPSEGGTGVTLVKI"
     gene            complement(12304..12846)
                     /locus_tag="PK015_05870"
     CDS             complement(12304..12846)
                     /locus_tag="PK015_05870"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOL34030.1"
                     /translation="MGFIDWLILAILIFFTIQGFVKGFIATLVRIGGAFVAFLLIGQI
                     FPLVRNSLIINLHLGRVPSSILAVILLVIIILVLLRLIAWILTKVIKAMHLSFPNKLL
                     GMIFGFANGLLIVIVFMVALDYFPSVTIPLKDGSKHRVYAAVDTFKEDIFTQLKFSER
                     DRFHQISETLKEKEKKASSK"
     gene            complement(12928..13134)
                     /gene="rpsU"
                     /locus_tag="PK015_05875"
     CDS             complement(12928..13134)
                     /gene="rpsU"
                     /locus_tag="PK015_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002551877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOL34031.1"
                     /translation="MPTVVAKENESFDYLLKRFKKKCEKAAILSEIKKRQAYEKPSER
                     KKREANVARRKMIKLQRRMQRYMR"
BASE COUNT         3978 a         2704 c         2219 g         4261 t
ORIGIN      
        1 atctaatatt accagattat atacttacaa tgaagagggc tatgccaaga gagttgatga
       61 tatatctaca aatgcagaaa cactatatct ccaggaagga aactggtggg aatttgaccc
      121 gattgatcct atctatcaac cacaatggtt atgctatgac ttagaagcct ttcatgaaca
      181 aaatctcatt ctttattggc aacctcctat ggctgatggg atacatcaat ggacttccta
      241 tcgtatttac gattcttata atcaaataac ccctcaatta atagcggaag ttcccttttc
      301 tcagactttt tggcagagaa atataactta tactgaccat tatatcctaa ctgtttgcgc
      361 ctttgatggt aacactgaaa gtttacctac taattatgtc atagttaatc ctactgctta
      421 taatgaccct atagctacac ctccagttct atctattcat ccaaatcctg tttgtttttc
      481 cgttaaccag caagtagaaa tagctattaa aagcgatgtt aatttaaaag gaaaaatcaa
      541 tatctataat attcggggac aagtagtaaa aactattccc ttagataata aaggagattt
      601 ttcttatcat tggaatggga aggataataa gggaactcaa tgctcttcag gaatctattt
      661 tgttaaagtt aaaatccagg gagctcctct cttgcaaaag aaattggtgc tgatgaaata
      721 aactttctta tcacttaccc ttaattcttt agaccttata aagttatcta taaattgagc
      781 agtgattatt aaaattaaac tgctgaaggt taaaaatatt tacaattata accataatcg
      841 ggagtaaatc taatgttata tttaccgagt agaatcttct ttggtgagaa tgccttaacg
      901 gaatctgagc tctatcttat aagactggga actaaacctt taatcgtaac cgggaaaagc
      961 agtgcaaaat taagtggtgc tttagatgaa cttattccca ttttaaagaa ggctgagcag
     1021 gattatgcca tatttgatag agtgcaagaa aacccttctt gggatatagt tagagaaggt
     1081 gcaaggtttc tgaaagatgc tcagtgcaat tatcttatcg ctatcggagg gggtagtcct
     1141 attgatgcag cgaaagctat ggctctaatt actgcaaata atctctttga agaagagaat
     1201 cttaccccgg ataaattttc tcaaacccta cctctggtag caattcctac tactgccggg
     1261 acaggaagtg aagtaactca atattctgta ttaactgata ccagcacagg tataaaagca
     1321 ggatttggaa cggaactcgc atttcccact ttggctatct gtaacccacg ttatactgta
     1381 agtttaaatg ctactatcac acttcatacc gcaattgatg cactttcaca tctcttagaa
     1441 ggtatctttt cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntctactatc
     1561 cttatataaa agaagaatta gataaccttt tctctaactt aaacattacc cgggaagatt
     1621 ttgaaaactg gctggataga ttcccattaa aggcagaatt tgacctgagt gaagagttta
     1681 tagaaagcca tatagcggaa gtgctggcaa gcagaaatat ggcaaacaca cctatcaagg
     1741 tgaaggaaga ggatatccga aaaatttata aaagcctgaa ataaaattta gttaagcatc
     1801 cgtctccgaa aagatactcc ttaaatccca ttgaataatc ttttccaaag gaagaccttc
     1861 ccgccaatca gtaccttcca taataggttc ttccattgct ctggatataa tttttattgt
     1921 agcttttata gaagcatcaa aatcataatc attaactaaa ccggaaagca aaagtgccga
     1981 aaaacaatct cctgctcctg gatagatacc atctttctta acaaagggat aggtgcttaa
     2041 tttgtctaac tttgcttgat aatatataac cttcagacta tcagaaaccg agccaggtac
     2101 agaagttatc actatgtgtt gtggaccata ttcactaatt tttcggcacc aatctaaaag
     2161 ttcattttct tcacctattt ttctaattac tccttttgcc agccaggcag cttccgagta
     2221 attgggagtg ataacatctg ctaaactgat aagttctcgc atagcaacta tcatattatc
     2281 gtcataacag gaatacaatt taccataatc gcccatcaca ggatcaatca gtactaattt
     2341 aactttttct ctccaggatt ctatcacttt ccctatttgc acaacttgtg caggtgaagc
     2401 cagaaatccg ctgctgatag catcaaaaga aagtcctatc tcctgccaat gtgcattaat
     2461 tttatccaaa taggaactca tatcaatcca tattggttca ggataatccg tgttagcgga
     2521 aagataggca gaaggaagag cacaaactct aatacccatc ctatacataa tagggatgca
     2581 aaccatcaga gaagtgtgtc cgaatccaga aaaatcgtgt atggcaagaa ttcttttaca
     2641 tttatttccc ataattatat tctatcctat tacaaataat caaatatttg ggttttgtta
     2701 agcagcaaaa aaacggaaga aaatctcata taaaacctaa cctaatttaa taataatcaa
     2761 agagttaagt ataatatttg tccttataaa ctcttaactg cttcatttta aagaaaatac
     2821 aaactctccc ttagctcttt cttagctctt ccttagctct tttgataagc aggagataag
     2881 tgtgagataa gagagaaaaa ataccagctc ataattctga aaccaacaca tcaaaactat
     2941 ggtaagaaac aagtttaagt ttgtgatgca aactgattgc gataattttt caccatttcc
     3001 aggtcataca tacccttata atgttccctt tctgcttgag gttggtaatg atagatggta
     3061 gcaggagtga aatgtcccag tgcaataccg agagccatac agatagcatt tggtgaactt
     3121 tgaaagatat gtaatctttg ccagtgataa ctgtttttca gatagttcag caaagaattt
     3181 gtctcttgca caattctcag ccaatccgag tccagattga tttgtccctg actttctttc
     3241 aaatagacga aaagagttcc agaatcagga aataagggtt taaaatagct aattacttcc
     3301 tgataggggt tatgactacc aaaatataaa atgagagcca ggtctttcat ttgacaggat
     3361 ggtgcattta tttcgtactt gatatgagtg aaagaatggg gatcaaagga tacatttttc
     3421 aatattcggg cattatttgg acgataaagt ctgaaaactg gaagatattc ctggttagta
     3481 ggttcccagt gacagatata tattggacga ttgaatccga aaatagcacc gataccaaat
     3541 tgaagagaac ttagcatacc ggcataccag aagagagctt tccgagagct gagcttttgt
     3601 tccaccctat caaagtggtc tatacttccc aaaaaagtct gccatacttc tgggataaag
     3661 ggaaaatcac cttctttaaa aatagaaagg tcatcctcat tcaagcctgt gaactcaaat
     3721 aaagcttgaa gattaaaaag aggatattcc ttttggatag cagcttccat ttttagtaac
     3781 caggaagact ttttatctgg tgttctgcta ctttgcacta caggaaccgg aagttcggag
     3841 ctattaagcc agaattccag ttcttctaag gatttaacgg aggtaatcaa ttttttcccg
     3901 cgggcttcac aacacttttt tttctcagct aattgtgcag gaggcataat cttacctgat
     3961 tcatttacgg caccggtaaa acaaaaatcc ctcaattcat cagggttatc cgcgattaaa
     4021 gcagccgcag ttgcagccat ccaggaatcg cctacaaaat cttccgtaaa taccattaga
     4081 aaacttctcc cggtcaattg agcaagcata ttggcttgtt cagcatagat tttattaaaa
     4141 cagatggctc tggattcggt tgaactgaag ggaaaaataa gaacttcagc gaggactcca
     4201 ttactttgtt ggtccactac agggaaagaa gcttttctta agcgggattg aaaaagttcg
     4261 gaaaattgac ctggattcaa attaagactt ttagccagct cttcatcctg atgtgaatta
     4321 gcaattaaag caagccaggc taaggcacta accgtattgg aataaggatt aaaaagcaaa
     4381 tgaataatag aactccagtc ttcttccgaa aaattattac tgttcagata gtccactatc
     4441 ttgttcttca gcaggtcatt gccctcattt aaataggcag aaacaagttc aggaaaagtc
     4501 attttttctc ctcttcttct atttcaatgc tgatatttaa ccattcttga atggatttca
     4561 ggtctttaaa acctctcaaa cctaaccaca attgtttggg gatatagcct tcaaacacgc
     4621 actcggtaca gatgtaaatc ttccccagtt tacctatgta atcctcatct ggataaagta
     4681 aaatcccgcc atcaggttct tttaccaggg tatgagttag cgcataaaag atattagaag
     4741 tttctggttg cctcattaaa tcaaaagctt gggttaaagt tggtcgggca gcagcatatt
     4801 tattcattgc cagcccttct ttctttacca gcaaatctac catcggtgtc aaatctaatg
     4861 cattcgcatc caggtctcta aagcgaaaat agctggttaa ttgatactct ttgaagcgga
     4921 attccaattt ccatttatcc tgaatagcag agtaatctat ccccttcccc tcttgctttt
     4981 cctttatgct tttctccaga gcttcctgca attcaaagat agtcattaaa tccagataat
     5041 ccagcactac actatcttct atttcttcag gataaagcaa gaaattgtta tatgggtcaa
     5101 ttaaatattg accgctatag ccaggagcga taatatcagt atccagtgca taatgaataa
     5161 aaggactgac tttcataacc agataaagtc cattttcttg aagcgctact ggaacataat
     5221 ttataagctt tcctgagtga cttaactgac gaatagtccc caaaacttga gtttctatcg
     5281 gttctatatt attctgttca actataatat tggcttcacc tgcctctgtg gtcaagctct
     5341 ctattttctc tttatcttca tcaaaatcaa atctgtcttc taaggagtcc agatagattt
     5401 caaaaagttc tttttcaaac tcttcgtttt tcataggagt tcttccgcct ttgttttctt
     5461 attatataga tatgttggag aaggtggagt ttcctgacat aattctggca gatataactt
     5521 aaaaaaagtt ctaatttcat ctttggtgta ttgggtgagt aattcattaa agtcattccg
     5581 taatcgctga tgcagtttat aaatattatt ttcactttct gccttaagtc tttccgtttt
     5641 aatcccgttg accttatcat aatattcaca gagcaggttt attttattga gggaccattc
     5701 ttgctttatc tggatataca acttttctgc acccctgcgg ttctcttctt ccagaataat
     5761 ctcgcccata gttttaatct tagcaggatg atatatttct ttgcaggaag aattcataag
     5821 attatcatca taattagatt cagcagattc caaatctttt attatcttat tattgcattg
     5881 aattatgata ttttgaaaaa ctcgggttag ataacgatac aactgttcac cattctttcc
     5941 ttccaggttt ttcttaagcg aatccaactg cggacaaatt aaatataaaa aatcccctgc
     6001 tctacttact cttgttaccc aatagcttat gtttctttta gagcataaat cgtaaatgag
     6061 cttttcttgc tttttctgta gagaaggaat taatgagatt agataaaata tatcggtatc
     6121 agagagctct ttctcaggat aaagctggaa tatttgccag agtttagcta taatcttttg
     6181 ccagcttcgc tcttctaaag ataaactatt ataccaggac tgatatttat tctggtcaca
     6241 actatagagc ataattcaat cctcagcagt agctattagg ggaaaaaggg aagaaaaaca
     6301 atggattaag gaaagtgaaa ggttcaatat tgatagtgta aagggaagat taggtatttt
     6361 ttctgccact ttatcttccc tgtttattta ggaccgatgg gtttgtcctt ttacaacagg
     6421 gtgcagtgtt taggcactgc actctgttct tgaaggtaaa ggacaaattg gagtatatca
     6481 ctttacatta aagttatcca gaaactgttg taaaacggct gcacgagacg gatgacgcaa
     6541 ttttttaagc gctttttctt ctatctgacg cactcgttca cgcgtaacct gaaagatttt
     6601 tccaacttct tccagcgtgc gatgataacc atcatcaatg ccaaaacgaa gacggataac
     6661 tcgctcttca cgagtggtga gagttttcaa tacctcatcc acttgctttt tcaacatagt
     6721 gcgttctgcc aatctttctg gactgatagt ggtacggtct gggataaaat cactaagcac
     6781 actatcttca ttatcattac tgataggttt atccagagaa acgggttctt tagcaataga
     6841 aagcacactc ttaacttttt ctactggcat atccagaact tctgcaattt cttctggata
     6901 gggatcacgc ccaagtttct gttgcagacg acgcgtagtt ctattaatct tgttaataga
     6961 ttcaatcata tgcactggaa tacgaatggt ccgagcttga tcggcaatgg cacgagtgat
     7021 agcttgacga atccaccagg tggcataggt gctaaattta taacctttgc gataatcata
     7081 tttttccacg gcacgcatta atccggtatt tccttcttgg atgaggtcta agaattccaa
     7141 ccctctatta ttgtagtgtt tagcaataga gataaccaac cgaacattag cttcaatcat
     7201 ttcatgctgt gctctttcct tatttctttc tgctctttct atttcccgga gcaagaaaat
     7261 aaggtcggaa ccagtcatct tggtttccag ttccacccga cgaatcttac gacgagcatt
     7321 cttaatcttg cgcagggtct caataaagat ttcggggtct ataccggttt cttttctaac
     7381 ctcttcaaag tcttcgggag atttttttgc ccgacgacca taagtgcata tctcatctat
     7441 agtatagcgt tctattcggg ttaactcgtt aatagtggca taactttctt ctattctttc
     7501 cactaaactg cgtatccgat agaccattcg cttaataagt atctcattat aggaaagctg
     7561 attaaaagtg ttaacaatat cattacgtag agcttcaatt tcctcctgac gagtagcatt
     7621 tccactttca tcataatcgc aactatctaa gatctcacca atcttattga aggtagcttc
     7681 atttttcttc aatatctctt taaattgctc aataataacc gcatcctgtt ttttatctat
     7741 ccaggttccc aggtctatat ggaaaatctg gtccagacga actctatgtt cacgataacg
     7801 atgcaagaag ttatacattt cccgcaaagt agagccagac ttaaatacat tctgattaat
     7861 cattttctgg taggtctcaa ttttttgtgc cacccgcact tcaccttctc tatccagtag
     7921 aggcactctt cccatttctt ttagatacat acgcacgggg tcttcataat agcgtttatt
     7981 cttaaacttc ttgggcacat taggtttttt aattgctaca ccacctttgg tgagcctttt
     8041 ttctgtttca tcaattattt ctatgccttc ctgacccagg tcaaagatga tgtcatctac
     8101 cttatccagg aaaaaggggc tattagggag gatatcgtta atctgtttaa atgtgatata
     8161 ccctgtatcc ttacctaact caaccagttt tttcagacat tcattgtaag ttatcattgc
     8221 ttctccttat cgggtttaaa aatctatgta aggtctttat ggacaacctt actggtcatt
     8281 tgccggtaga gagcaaccag cttgctcttc tccttcagca aagttttgtt ttcgggttct
     8341 gccataatac gcctatcaat atccctcaaa tctctttgta tctttctaat tttcacctgt
     8401 tctaatacag acataaagtc caaatcctgc atttcttcaa agagaaaatc tcccactaat
     8461 cctctcaact cctggttttc cattaaatcc agtagttgag ccggttccga aatcatatta
     8521 gccgaagcat tattaaccag atacttaaac aactctttat accttttatt gttaaaataa
     8581 tcgggagata ggtcttgagc aagtaaatta aaattatcgg ggtcctttag agccaggata
     8641 agtaattgtc tctcttcagg atattcctct aaagcaggag tggaatataa tccctctgtt
     8701 tctaccacag aagtcctatg caactttgaa aataaagaac gagtggaaac attgaaagct
     8761 tcagatatat ctttcactat cagttctcgt ttgatagggt ctttaatgga acgaaccgca
     8821 tccagaagct ggtctattct ttccataagc ggagttttag atttagcttc agccataaaa
     8881 tgtattacat cctttgctgc agctattctt tcctggagtg cttcaggtcc attttctaag
     8941 agaaaactat ccgggtcatc tttttctggt agttctataa tgctggcatc cagacctttg
     9001 gataaacata ataaagcagc tctttgagca cttttaattc ccgccgcatc tccatcatac
     9061 agcatataaa ccttattggt atatcttgcc agaagataaa tttgatcttc cgttaaagaa
     9121 gttcctaaag atgctacggc attctgaaat ccgttttcat agagccttaa aaagtcaaag
     9181 tatccctcac agattagagc ataacctatc ttactgattt catatttggt cttgaagaag
     9241 ccataaagtt ctttcccttt ggtgtataat tctgtgcccg gagtattaac atatttacca
     9301 acattttcat tttcttccag aattcttcca ccaaatgcca ccacttcgcc aatgctgtta
     9361 tggattggaa acatcaatct attccggaaa aaatcaacca gattgccgga ataatttcca
     9421 aatagtccag aatctttgag caaatctact gaataccctt tatccagcag ataatttagc
     9481 aaaatctttt cgccttcagg tgcataaccc agctccagag ttttagctgt ttctggactg
     9541 atggcacgat tttgaagata ggatagcacc tccttttgtt tcatcaactg attagtaaag
     9601 aaatccctgg tttcagtata aatccgcaat aattgcgctc ttttagttga tgcaggagaa
     9661 attctttcct tttcagggat aacaattcct gctctttgag cgagcttctt tactgcttca
     9721 tagaagctca ttttttcgta ttcctgaaca aaggtgaaga catttcctgc ttttccacag
     9781 gcaaagcact taaaaatctg cttgggttga ctaacataca gggaaggatg agtgtcatta
     9841 tgaaaaggac agataccacg ccagtttgtg cccacctttt tcagtggcac atattcctga
     9901 actacatcca ctatatcgtt acttctcctg atttcttcta tcagattttt atccataatt
     9961 aaatcttaac cagcgtaact cctgtccctc cctcactggg taagggagtt tccagcgata
    10021 taacctgttt tttcttcttt aaataatctc gcaccttagt tctcagagca cctgtgcctt
    10081 taccatgaac aatacgcagg gtatgcaatc ctgccagcat agcatcatcc agaaattcat
    10141 ctattaaggg ttttgcctct tcaaaggtca atccaagcag tttcaattca aactgagctt
    10201 taggaggtgg attggaatga gtgatgacca ccatttcttc cttcttggca ggcttcccta
    10261 tctcataaag gttatccaga gcagtcttaa aagtaatgcc attcaaatca actattgctt
    10321 gctggtcttt aatctccagg atagtaacct cagtttccag ggtggctaac cagactctat
    10381 caccttctct gggaactctt attttttgcc tttgctcaat ctcagaatcg cttaactctt
    10441 tattaatatc ttgcaatcta ttattaatat cttgcaattt cctttccgaa actgactttc
    10501 tttcttctct ttccatcttt ttcagggatt cctgctctct ctgatacagc ttttgttgag
    10561 ctattagttc tgattgaagc tcctgtaaat atttctttct tcgctctttt aactctgcat
    10621 ttaaagcagc ttcccggctt tccagttcct tcagttttga ttccagattt cgtgttttga
    10681 gttcatactc ataagtagct cgtgccagag ctttcttctc ttcctgcatc ttcttgagta
    10741 gctcagtaaa ttctatattt tgagaaccac tcagttcctt tgctcgctca attaaatcag
    10801 gttccaatcc gagagaagaa gccacttcaa tggcaaaact atcgccagga ataccagtag
    10861 taaaacgata agtgggagtc agacttttca aatcaaactg cattgaagca ttcagacaat
    10921 ctggatgagt ttccgcaaaa atctttagag cagtataatg agtagtaacc acagcctttg
    10981 atccaagctc agttaagcgt tccagtatag cttgagctaa agcggaaccc tgttgagggt
    11041 cagttgctgc tccaatttca tcaattagca ccagactctt ttcattcgca gatttaagca
    11101 ttcttcctat ctttccaatg tgagaagaga aggtagaaag ggcattttct atagattgct
    11161 catctcctat atcagcaaag acctgattaa atctaccaat aacactattc tcttccgctg
    11221 gaataggtaa acctgaaagt gccattaagg tcaataaacc agtagctttt aataaaactg
    11281 tcttaccacc cgtattgggt ccactaagaa gtatgattct tgctgtttca cctaaattca
    11341 ggtcaaaagg tactactttc ttcacatcat tcaaacttaa aataagcaaa ggatggcgag
    11401 cagaataaag ttttaatact ggttcatcta caatctgagg cttttttgct tttaaagtgt
    11461 agcaaagtcg ggcgcaacca aaacggaaat ccagctccgt taaagcttcc tgattggcaa
    11521 tcaggatatc cttttgttct ttaatctgag cagtgaattc attgaaaata ttgaatattt
    11581 ctctcttttc ttcttgaagc agaagctgaa gttcattatt caaaggaact acactttctg
    11641 gttcaataaa aagagtggaa ccgcttccgg attgtccctg aactattccg ggaacaaaag
    11701 caccagcatt accttttaca ggaagcacat atctaccttc ccttcgggta ataaacttgt
    11761 cctgcagaaa attctcataa cgagcatcat tgagcatatt ctgcattgtt ttctggatgc
    11821 tacgctgtaa tgaagcaaat cttttccgga ttctatttag ctctactgac gcagtatcca
    11881 gaacctcacc ctcaggagca aatatctttt caaagcgggt ctttatctca ttcagaggtt
    11941 tcactttttg ccacagcttt ttcagcacag gaaaatcttc tacactttcc gctttctcac
    12001 ttaccgcttc agccagggaa acttgtaaat atacctgctg aaattcttca aaaccaaaaa
    12061 gagaatatgt agcttctgca aaaagaggtt gcagattgga taaattagtg aaattgatgg
    12121 agattccacg atttagagcc tcttgaagct cggaaacaag ttccagcgat gcctctatct
    12181 cttttttttc tgatagaggt ctaagctgtt ttgctcgttc cttgcctaaa atactctcac
    12241 atcgtgtata cacttcttct actaatctgg aatattccag gtctgcataa agctctttat
    12301 cgttcattta ctggaagctt ttttctcctt ttcttttaat gtttcactta tctggtggaa
    12361 acggtctctt tcggaaaatt tcaattgagt aaagatatct tctttaaaag tatcaaccgc
    12421 tgcatagacc ctgtgtttag acccatcttt cagaggaata gtcacgctgg gaaagtaatc
    12481 taaagccacc ataaatacta taactattaa gagaccattg gcaaacccaa aaatcatacc
    12541 taacaacttg tttgggaaag aaagatgcat agctttaatc actttagtta aaatccaggc
    12601 tataagacga agtaaaacca gaatgataat aacaagtaaa attacggcta aaatggaaga
    12661 aggaacccgt cccaaatgca aatttataat caaactattc cgaaccaggg gaaaaatctg
    12721 acctattaac agaaaagcaa caaaagctcc accaattcgt accagcgtgg ctataaaacc
    12781 cttcacaaat ccctgaatgg tgaaaaatat aagaatggca agaattagcc agtctataaa
    12841 acccatttct tctcctcaat aaaaaagcaa acagggacat tccctgttgc caatgggtta
    12901 ttttttggat aaaggataat tcaaatatta cctcatataa cgctgcattc tgcgctgtag
    12961 cttaatcatt ttacgacgag ctacattagc ttcacgtttt ttcctctcgc ttggcttctc
    13021 gtatgcctga cgcttcttta tctcagaaag aattgcggct ttctcgcatt tcttcttaaa
    13081 ccttttcaat aggtaatcaa aggactcatt ttctttggct acgactgttg gcaatcaact
    13141 cacctccttt ttttattgta aaattaactt ataatttaac attatcttat ctgtcaagat
    13201 gaaaatggta agactaaatt atatcttttc taattgatac tataatagat actatgattc
    13261 ct
//
LOCUS       DAORZF010000045        13041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_3744_length_13041_cov_383.441860, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000045 DAORZF010000000
VERSION     DAORZF010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 13041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13041
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3744_length_13041_cov_383.441860"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..979
                     /locus_tag="PK015_05880"
     CDS             44..979
                     /locus_tag="PK015_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34032.1"
                     /translation="MKHERKITQKMVEAALIMKQINGEMIQTVPNNVDLKYVYCANFT
                     KSVINGVTPEMFCCELIRGDGGELRNASGSAPKFNSIVSSASMAVSTFAPWKNRLSEL
                     YIDLGSQQLTGFKKLEFEHIAKTAIPRAKKHPNIDVWLENDDTILAIECKFCEFLSKR
                     EEKASLNPSYKRFATEQKMHSPWVEAIDVVTNKNGISKYQFFDAVQIIRHYFGLLNSG
                     QKEKHLLYLYWHPDNEDWMDIHPYGTLLNELKEFSDFVSIATDVKFHYIGINELWEKW
                     CLSPNSQVKDHVKELMKKYSIKINNYFPQKRSYKC"
     gene            1069..1575
                     /locus_tag="PK015_05885"
     CDS             1069..1575
                     /locus_tag="PK015_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34033.1"
                     /translation="MSRDLFKKKKLLLNHSNQQLQIELIGYEVPLGTNINDQQFIDLV
                     GLDESHVLYIIETKNNNSKEKPSDVVKNQINKYYERLKKSLPYIVAEINQNEEHRDVK
                     VDKITKILLAPLIYFQKHESNIELVDDDVLLCYFAKSIEYSDITSVSDDDGSIRLCIY
                     TPDSLGNR"
     gene            2287..4935
                     /locus_tag="PK015_05890"
     CDS             2287..4935
                     /locus_tag="PK015_05890"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOL34034.1"
                     /translation="MEISKTYEPQQIEKKWYEFWEKNDYFKPAGDPSKPPFTVLIPPP
                     NVTGILHMGHVLNNTLQDIVVRFHRMLGEPTLWLPGVDHAGIATQNMVEKELAKEGKT
                     RQEIGREKMLELIWKWKEEKGSHIIDQLKMLGASCDWSRLRFTMDEMLSRAVKEVFVR
                     LYEEGLIYKGKYIINWCPRCVTALANDEVEYSEEDGNLWYIRYPLTDGSGYLVVATTR
                     PETMLGDTAVAINPRDERYKHLVGKEVELPLTGRKIPIIEDDYVDMEFGTGCVKVTPA
                     HDPNDFEIGQRHSLPQILIMDEQAIMNSAAGKEFVGLDRFACREKVVELLTQQGLLEK
                     IESYTHSVGHCYRCDTVVEPYLSDQWFVKMKPLAGPAIEVVEKEEIRFQPERWTKVYM
                     HWMNNIRDWCISRQIWWGHRIPVYYCNSCGKMIVAKEMPSICPDCKNNSFHQDEDVLD
                     TWFSSWLWPFSTFGWPEETEDLKRYLPTQVLITAPEIIYLWVARMIMSTLHFVHIIPF
                     QTVLLHGTVRDEYGRKMSKSLGNSPDPIDIIDRVGADALRFSMVFGTPKGADVIFSES
                     ILETGRNFANKIWNAYRFIMMNVTEREQLPPKEELQMELADRWIYSRLNETVRNTAEH
                     YQNLRFNDAAQSIFQFLWDEFCSWYIELSKDRLNSNDALSRSTTLYILLDVMQTSMRL
                     LHPIMPFITEEIWQNIKKVFNQPEESLIIAAFPSCKEKMIDPEIADDMSFMQEAITAV
                     RNLRKELNLNPGTTISLVIRVAEERQKDLIERYATYFRRLAKVENIEVGLQLPKPKPS
                     IAAVVRNLEIFLPLKGIIDLEAEKARLSKQVEKLEKELQSVKKKLDNPHFLANAKPEV
                     VANEHKHFEEVNIKLSLTKELLEDLK"
     gene            4973..5048
                     /locus_tag="PK015_05895"
     tRNA            4973..5048
                     /locus_tag="PK015_05895"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5006..5008,aa:Ala,seq:cgc)
     gene            5387..6874
                     /locus_tag="PK015_05900"
     CDS             5387..6874
                     /locus_tag="PK015_05900"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOL34035.1"
                     /translation="MKTKCLLFVLLALTVLLSAQEISTYLNFSHSSLCPDGYLRLRWL
                     DETGNSAATQCFYSLNGSDWQYTSASSLQGNQMEAVVPYEFGQSLRYRLRTPVNIEGE
                     QIVFMHIPYLTSDVFPPSLSQLGELSTDPTGDSDIPDIPALDLTDSWCGVSETKLYSA
                     MANAANAFPLVHNITSYNLFATFIFNPETIIDTLCYAMIYTFNIPSVISPGLYKMGID
                     LEGVGPTSFVRIGDIQSTVSNGKLIMACNMSDLINDPDFGTWPNLTNALIFTNISVNF
                     GLLQQQPYFSQADFTYPGFLLFHNFHYQVDQNTLPQLTNLTYNSNTHLFEFDYTDNEE
                     DFPLIMEVELAGGQILNPITLNPDYSQTVHYSLLIPNPSQYITLRCSDNLSDIVEVVY
                     DTTTVNDDILPVPALKCHMPNPYNPLSGPCNITIKGLDKKPVKVELFNLKGQLLGTLY
                     EGNPLDDKLMFSFDGSMKNLKLNSGIYIIRIQQNQRNLLHKFILQ"
     gene            6897..>7423
                     /locus_tag="PK015_05905"
     CDS             6897..>7423
                     /locus_tag="PK015_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOL34036.1"
                     /translation="MFIDYAKITIKAGNGGDGIISFRKEKYISRGGPDGGNGGHGGSV
                     IAIGDENVNTLLAYKYHRKFYAEDGKPGSNARKRGLSGDDIYLHLPLGTEIYQITETG
                     ERIKIADITTHNEEIILAHGGRGGKGNSNFATSTNQAPRIATPGRKTKEIVLELVLKL
                     MADVGLVGFPNAGKST"
     assembly_gap    7424..7523
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7524..7718
                     /locus_tag="PK015_05910"
     CDS             <7524..7718
                     /locus_tag="PK015_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOL34037.1"
                     /translation="DKKPSLIVLSKLDTIPEEERNARSAELQSLFQKEYGLDVLPISA
                     VGNLGIEELKWTLYNMLDKK"
     gene            7715..8806
                     /gene="dprA"
                     /locus_tag="PK015_05915"
     CDS             7715..8806
                     /gene="dprA"
                     /locus_tag="PK015_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOL34038.1"
                     /translation="MTDKFKAWLCLKSAFGTKPKTALELVKKYPHPEEYVGNSQHPIY
                     EDDTIPPQIKEQLLSAVPHPREEQIAKLCEHYEINAIFYGDEDYPLALANIILPPLIL
                     YYRGDLLKALNNISLAVVGTRKPTAYGKNACSKLLAPVCQREVTIVSGLAYGIDTVSH
                     ITALQNGAMTIAVLASGVENIYPPANRELAKEIINNGALVSEYEPGTKLESWNFPARN
                     RIISALAPVTFVVEGSMTSGAMITAKHALEQNHDLIALPGEIYHPNAQGPNYLIKNGA
                     QCITCPEDILYALGIEVEEREQLEFLPEISPDEQKIYDLFREEQRELSFDELLLKTGY
                     SFGKLSTILLNLELKGLIIKSGGNAFLLS"
     gene            9121..11004
                     /locus_tag="PK015_05920"
     CDS             9121..11004
                     /locus_tag="PK015_05920"
                     /inference="COORDINATES: protein motif:HMM:NF040561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated primase-polymerase type A1"
                     /protein_id="HOL34039.1"
                     /translation="MKEIIINAEQLEQKLPKTSRFHQYRIAFNSAFLYEDFATASKVC
                     EEISALPELTVNERCELASYFQMLGEIDAALKQLDLVLQANPEEHNAALQKARILRLH
                     QDSEKYLSFIQSRIIDFPSFAPYYLDVWEFLKTNPNNELRELIQQLAQENSIVLPEEK
                     EEFILSPESSTPFSQTDIYSDSDLLTMINLFQGRENSYARQWVSDEGKYGYTPVNEPL
                     NLNAVRNHLLGIQTLGIYQLDLMGKVKWIVFDLDVEKSHLDNLHDPAFRLWLEQGFQK
                     IINEFRELLKTYHLEMNVEFSGYKGYHLWILLKEKIPAYVARSFAQRIVAQITFNGLP
                     ISIEIFPKQVRTSRDNYGNLVKLPYGIHRLSGLNSTMLNEEFKIISFEEFIKQPKMVS
                     PEDLISALFALDPNFSLIQEKENAQEPVSPLIYPETLEAPDPETDSEWLCLKQNCAVL
                     WTIDNLISTNHSLTGEQKTVLRYVCGHLTNGPAIVNTLMKKLTNCELDDLMKRPFRGN
                     VMSCNRIRSLVGTLVEPERCHCDFSQESGMYPTPLLHLKKLSSKSINALQANDLMLKD
                     LISTYLKLKSQTRDLQERLEQVEKDIINAFENIGLDEISTPYGILKKITNGEQISLVM
                     QLK"
     gene            11019..12308
                     /locus_tag="PK015_05925"
     CDS             11019..12308
                     /locus_tag="PK015_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34040.1"
                     /translation="MNNTEFLNELEQAKQLARSGELDIALQIFEKLYAADNNEQNLSL
                     IYYMQNSLKQAANYSKAIEIGLVACELPEVWDSIRHNTAWCIYFQYFKEKCELPVEEA
                     IEWLEKIKELFPPAPGLHPLPLSVFSYINKHPELSPALIIQLCSLLDPQKLEMEPVPA
                     KDGKYSLPSQREKYIDLLSKAYIAAGNYNDCLELCQQTLNEPITLTPAKHIWLKRRIA
                     ICHSHLGEEEKALNEMKEILLSKKDWFLFYEAAQIAFNLKNIDEAVDFAIRAALAPGE
                     IENKIHLWELLKSILFQLKLFDKAIEMLQLIASIRKFKQWSIPQSLNKELNSYHISLD
                     NLQHYKELFSQIRKWLPELLTNDAEELSGTINLLLPHKKAGFISCGKESYYFRTADCQ
                     IPVDELQHGLKVKFILQPGFDPKKQKETIVAVNIHRE"
     gene            12311..>13041
                     /gene="cas1"
                     /locus_tag="PK015_05930"
     CDS             12311..>13041
                     /gene="cas1"
                     /locus_tag="PK015_05930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HOL34041.1"
                     /translation="MSVVYVADQGASICKRGDRLYVFKGPNLLRWFHSKDITQLIIVG
                     NVTLTTPVLTYLLKYKIDTVFLSYYGKYKGRLIGEFGKNVQLRIAQFQFLNSLQNRES
                     LAALYIQGKLNNMQTHLMHRLKRKKDKLISTAILQNQAILDKIEQSSPNLDTLRGYEG
                     IASKNYFSAFPALISNPQFIFTGRNRRPPKDEVNALLSLGYTFLMNQVMGATYISGLD
                     PYYGALHDIAYGRQSLALDLMEEFRP"
BASE COUNT         4131 a         2531 c         2344 g         3935 t
ORIGIN      
        1 ggacagataa agtaagtaaa agtataggat tctaggaggt accatgaaac atgaaagaaa
       61 aataactcag aaaatggtgg aggctgcctt gatcatgaaa cagatcaatg gcgaaatgat
      121 tcaaaccgtt ccgaataatg tggatttgaa atatgtctat tgcgctaatt ttactaaaag
      181 tgtgatcaac ggtgttactc ctgagatgtt ttgctgtgaa ttgattcgtg gtgatggagg
      241 tgaactgagg aatgcttcag gatcagcacc aaagtttaat tcaattgtat cctcagcatc
      301 aatggctgta agtacttttg ccccatggaa aaaccggttg agtgagttat acattgatct
      361 tggttcacag caattgacgg gattcaaaaa actggaattt gagcacattg ccaaaactgc
      421 tattcccaga gctaagaagc atcccaatat agacgtttgg ttagaaaatg atgatacaat
      481 tcttgctatt gagtgcaagt tctgcgagtt cctatctaag agagaggaga aagcaagcct
      541 gaacccttca tataaaaggt ttgccactga acagaaaatg cactctcctt gggtagaagc
      601 gattgatgtt gtcacaaaca aaaatggcat ttcaaaatat cagttctttg atgccgttca
      661 aattattcgt cactacttcg ggctgttaaa ctccgggcag aaagagaagc atctactcta
      721 tctatactgg catccggata atgaggactg gatggatatc catccttatg gcaccctctt
      781 aaatgaactt aaggagtttt cagatttcgt ttcaatagcc actgacgtta agtttcacta
      841 tataggcata aacgagctgt gggagaaatg gtgtttgagt cccaattctc aagtaaaaga
      901 tcatgtaaaa gagttaatga aaaagtactc aataaagatt aataactact tcccccaaaa
      961 aaggagctac aaatgttaaa agaattcgat aacatccgaa ccaaagattt caaactattt
     1021 gattgtaacc atggcaaaaa cccaataaat ccaattgatg agcggtctat gtcaagggac
     1081 ttatttaaga aaaaaaaatt gctattaaac cattctaatc aacagttgca aatagaacta
     1141 attggttatg aagtgcctct tggcacaaat ataaatgacc agcaattcat agatttagtt
     1201 ggtctggatg aatcccatgt gctatacata attgaaacaa agaataataa ttctaaggaa
     1261 aagcctagtg atgtagttaa aaatcagata aacaaatatt atgaaagact caagaagtcc
     1321 cttccctaca tcgttgctga aataaatcag aatgaagaac atcgtgatgt aaaggttgat
     1381 aaaataacca agatactgct tgcaccattg atatatttcc aaaagcatga atcaaatatt
     1441 gaactggttg atgatgatgt attattgtgc tattttgcaa aatctattga atattccgac
     1501 ataacatcag tttctgatga tgatggttcc attcgattgt gtatctatac ccctgattct
     1561 ttaggcaata ggtaaaaatc agatgcttct ttataaagta tcagttaatc gtatgataaa
     1621 cagtctgtta aatactcaaa aactgaatac atatgacttt cgtcaaccta aacacccaca
     1681 gcgaatacag ctggctggat gggacctgca tcattgatta tctagtgcaa aactcttatc
     1741 tttgttattt gtgggaggtt ttttaattct tatttgaatg aaagttaaaa aaatacatat
     1801 ctgagtaatc agcatgcttt ttattcttat acagaacctc ttatctgtgt aatctgtgta
     1861 atctgtgaga gattttttac ttcttatctg tataaaaacc aaatctgcgt aatctgcgtg
     1921 ctttcattct tataccaaac ctcttatctg tgctatctgt gttatctgtg ggagcttttt
     1981 ttaattctta aaccatatct gcgtaatctg cgttatctgc gtgctttcat tctaatacaa
     2041 aacctcttat ctgtgtaatc tgtgagagcc tttttttaat tcttttcttg gagcttatgt
     2101 ccagcttatg tccaactcat gtccagctta tgtccgaaat cggacataag ctcactataa
     2161 tctcctttta aggaagagat aaacgctaaa gaagggcggc atatagttta tttgcttgac
     2221 atagggcgga tttatcaatt ttcggttata aaataaaaag gatttgttaa ataatagagg
     2281 atatgaatgg aaatcagcaa aacttatgaa ccccaacaaa tagaaaagaa atggtatgaa
     2341 ttctgggaaa aaaatgatta ttttaaaccc gcaggagacc catcaaaacc tcctttcacc
     2401 gttctaatac cacccccaaa tgtcactggc atcctgcata tgggtcatgt tctaaataat
     2461 acattgcaag atattgttgt tcgttttcat aggatgctgg gagaacctac cctctggtta
     2521 cctggagttg accatgctgg tatcgccact caaaatatgg ttgaaaaaga acttgccaaa
     2581 gaaggaaaaa caagacagga aattggaaga gaaaagatgc tggaactcat ctggaaatgg
     2641 aaggaagaaa aagggagtca tatcatagac caactcaaaa tgttgggtgc ttcttgtgat
     2701 tggtctcgtc ttcgttttac tatggatgag atgctttcca gagcagtaaa agaggttttt
     2761 gtccgccttt atgaggaagg tttaatctat aaaggaaaat atattatcaa ttggtgcccg
     2821 cgttgcgtga ctgctctggc taatgatgag gttgaataca gtgaagaaga tggcaatcta
     2881 tggtatattc gttatccttt aacagatggc agtggctatc ttgttgttgc caccactcgt
     2941 cctgaaacta tgttaggaga cactgcagta gccattaatc ctcgtgatga acgctataaa
     3001 cacttagttg gcaaagaggt tgaacttcct ttaactggca gaaaaattcc cataattgaa
     3061 gacgattatg tggatatgga atttggaact ggctgtgtga aagttactcc tgctcatgac
     3121 cctaacgatt ttgaaatagg tcaacgccac agtttgcctc aaatcctgat aatggatgaa
     3181 caagcaatta tgaattctgc tgcgggaaaa gaatttgttg gtttagaccg ttttgcctgt
     3241 cgcgaaaaag tagttgaatt attaactcaa cagggtttac tggaaaaaat agaatcctac
     3301 acacattctg taggacattg ctatcgttgc gataccgtag ttgaacccta tctttctgac
     3361 cagtggtttg ttaagatgaa gccactcgct ggacctgcta ttgaggtagt agagaaagaa
     3421 gaaatccgtt ttcaacccga acgctggact aaagtctata tgcactggat gaataatatt
     3481 cgcgattggt gtatctccag gcaaatctgg tggggacaca gaattcctgt ctactattgc
     3541 aattcctgtg gtaagatgat agtagccaaa gaaatgccct ctatctgtcc tgactgcaaa
     3601 aataattctt ttcatcaaga tgaagatgtt ttggatacct ggttttccag ttggctatgg
     3661 cccttttcca cttttggctg gcccgaagaa acagaagacc ttaagcgcta tcttcctacc
     3721 caggtgctca ttactgctcc tgaaattatt tatctctggg ttgccagaat gattatgagc
     3781 actctgcatt ttgtccatat catcccattt caaacagttc ttcttcacgg cacggttcgg
     3841 gatgaatatg gcagaaagat gagtaaatct ctgggaaatt ctcccgatcc tattgatata
     3901 attgatagag ttggagcaga tgctctacgt ttttctatgg tttttggcac tcctaaaggt
     3961 gctgatgtaa ttttcagtga gtccattctg gaaactggac gcaattttgc caataaaatc
     4021 tggaacgcct atcgctttat aatgatgaat gtaacagaaa gagaacaatt acccccaaaa
     4081 gaggaactcc agatggaact tgctgaccgc tggatttatt ctcgcttgaa tgaaacggta
     4141 agaaatacag ctgaacatta tcaaaatctg agatttaatg atgcagcaca gtcaattttc
     4201 caattcctct gggatgaatt ctgctcctgg tatatagaac tttcaaagga ccgactgaac
     4261 tccaatgatg ccctttctcg cagcaccact ttatatattc tgcttgatgt aatgcaaact
     4321 tcaatgcgtc tactccatcc tattatgcct tttatcactg aggaaatctg gcaaaacatt
     4381 aaaaaagtgt ttaatcaacc agaagaatcc ctcattatag cagctttccc ttcctgtaag
     4441 gaaaagatga ttgaccccga gattgctgat gatatgtcct ttatgcaaga agctatcacc
     4501 gctgttcgta atctccgaaa agaactcaat cttaatcctg gcaccactat ttctctggta
     4561 attagagtgg cagaagaaag acaaaaagac ctgattgaac gttatgccac ctatttcaga
     4621 aggttagcaa aagtagaaaa tatagaagtt ggattgcaat tacccaagcc caaaccctct
     4681 attgctgccg tagttcgtaa tctggaaatc tttcttcccc tgaaaggaat aattgacctg
     4741 gaggcagaaa aagcacgctt gagtaaacaa gtagaaaagc tggaaaaaga acttcaaagt
     4801 gttaaaaaga aactggataa tccccatttt cttgctaacg caaaaccgga agtggtagca
     4861 aatgaacata aacactttga ggaagtgaac ataaaactat ccttgacaaa agagcttctt
     4921 gaggatttaa agtaagcatc tatacataaa taaaatagat aaaatataaa gtggggctat
     4981 agctcagctg ggagagcgga acgttcgcaa tgttcaggtc aggggttcaa ttccccttag
     5041 ctccaccatc ttatcattta tctctcctgt tcttcctctt ttttgtatga tgaaatccat
     5101 atcaaaaaat tataaaaaaa ttcttgacga ctattattta attattaatt agataaatta
     5161 tgaggattat ctaactttat tagaaaaaag gggatattat gttgcaagaa tataatggca
     5221 gttttagaat tggaagcttt cctctcttgc tgctcaataa atgttttggc acaagtctcg
     5281 cacttattta cggtaagata aatcccctgc aaaattacta cttgttttat gcatatgatt
     5341 taatataata taaaaaaaca aacactcagg taaaaggaga ttaaaaatga aaacaaaatg
     5401 cctattgttt gttttgctcg cactcacggt gttgctatcc gctcaggaaa tcagcaccta
     5461 tcttaacttt tctcatagtt cactctgtcc cgatggctat cttcgtttaa gatggttaga
     5521 tgagacagga aattcagcag caactcaatg cttttatagt cttaatggct cggattggca
     5581 gtatacctct gcttcttccc ttcagggaaa tcaaatggaa gctgtcgttc cttatgaatt
     5641 tggtcaatct cttcgctatc gcttacgcac cccagtaaat atagaaggtg aacagattgt
     5701 ttttatgcac attccttatt tgacttcaga tgtctttccg ccttctttat ctcagttagg
     5761 tgaattatca acagacccta ccggagactc tgacatacct gacattccag ctttagatct
     5821 cactgatagc tggtgtggtg tctctgaaac caaactttat tctgcaatgg caaatgccgc
     5881 taatgctttt ccactggtgc ataatatcac ttcctataat ctttttgcca ccttcatctt
     5941 caatcccgaa actataattg atacgctttg ctatgctatg atttacacct tcaatatccc
     6001 ttctgtcatc tctcccggtc tatataaaat gggaattgat ctggaaggtg ttggacctac
     6061 ttcctttgtt cggattggag atatccagag caccgtttct aatggtaaac ttataatggc
     6121 ttgtaatatg tctgatttaa ttaatgaccc cgattttgga acttggccta acctcacaaa
     6181 tgctttgata ttcaccaata taagcgttaa ctttggttta ttgcaacaac aaccatattt
     6241 ctcgcaagca gatttcacct accctggatt tttattattc cataactttc attatcaagt
     6301 agaccagaat accctgcccc aactaactaa tctaacttac aactctaaca ctcacttatt
     6361 tgagtttgat tacacggata acgaggaaga cttcccgctt ataatggaag tagaattagc
     6421 cggtggtcaa attttgaatc ccatcacgct taatccagat tacagtcaga ccgttcacta
     6481 ttctctactc ataccaaatc cctctcaata catcaccctt cgctgtagcg ataatttaag
     6541 tgatatagtt gaagtggtat atgatactac cacagtgaac gatgacattc ttcctgtccc
     6601 agctttgaaa tgccatatgc ccaatcccta taatcctctt tctggtcctt gcaatataac
     6661 tatcaaaggt ttggataaga aaccagttaa ggtggagctt tttaatctga aaggacagct
     6721 tctgggaact ctttatgaag gaaatcctct tgacgataaa ttgatgtttt cttttgatgg
     6781 aagtatgaaa aatttaaaat tgaatagtgg catatatatt atccgaatac aacagaatca
     6841 gagaaatctg ctacataaat ttattttaca ataaaggagt taaccttcgc taagtaatgt
     6901 ttatagacta cgcaaaaatt actattaaag ccggaaatgg tggtgatggt attattagtt
     6961 tccggaaaga aaaatatatt tcaagaggag gtcctgatgg aggtaatgga ggtcatggcg
     7021 gttccgttat agctatcggt gatgaaaatg ttaatacact cctcgcttat aaatatcata
     7081 gaaaattcta tgctgaagat ggaaaacccg gttccaatgc tcgtaaaaga ggattaagtg
     7141 gagatgatat ttacctccat ctgcccttag gaacagaaat ctatcaaata acagaaactg
     7201 gcgaaagaat aaaaatcgct gatataacta ctcataacga agaaatcatc ttagctcatg
     7261 gtggaagagg tggaaaagga aacagcaatt ttgccacatc caccaatcaa gcacctcgca
     7321 ttgccactcc tggtagaaaa acaaaagaaa ttgtcctgga attagtacta aagcttatgg
     7381 ctgatgttgg tctggtcggt ttccccaatg ctggcaaatc tacnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7501 nnnnnnnnnn nnnnnnnnnn nnngataaaa aaccaagttt aatagtttta agtaagctgg
     7561 acacaattcc tgaagaagaa agaaatgctc gctctgcgga acttcaatct ttgttccaga
     7621 aagaatatgg tttggatgtc cttcccatct ctgctgttgg taatctggga attgaagaac
     7681 ttaaatggac tctctataat atgctggata aaaaatgact gataaattta aggcttggct
     7741 ctgtctcaaa tccgctttcg gtactaaacc taaaactgct ctggaactgg ttaaaaaata
     7801 tccgcatcca gaagaatatg tgggaaattc tcaacatcct atctatgaag atgatactat
     7861 tccccctcag ataaaagaac aactcctttc agctgtccct catccgcgtg aagaacagat
     7921 tgctaaactc tgcgaacact atgagattaa tgctatcttc tatggtgatg aagattatcc
     7981 tttagcttta gcaaatatca ttttacctcc tctcattttg tattatcgtg gagacctact
     8041 taaagcctta aataatatat ctttagcagt agtaggaaca cgaaaaccta ctgcttatgg
     8101 caaaaatgcc tgttccaaac tccttgctcc cgtttgtcaa agagaagtta caatcgttag
     8161 cggtctggct tatggaattg ataccgtctc acatattact gcactccaaa atggtgctat
     8221 gactatcgct gtacttgcca gtggagtaga aaatatttat cctccagcta atcgtgaact
     8281 tgctaaggaa ataatcaata atggagcctt agtttcagaa tatgagcccg gcactaaact
     8341 ggaatcctgg aattttcccg ccagaaatcg tataatatct gctctggctc ctgtaacttt
     8401 tgtggtagaa ggctctatga ccagcggtgc tatgattaca gctaaacatg ctctggaaca
     8461 aaatcatgac ctgatagcac ttccaggtga aatctatcat cccaatgctc aaggtcctaa
     8521 ctatctcata aaaaacggag cacaatgcat aacctgtcca gaagatattc tctacgctct
     8581 cggaatagaa gtggaagaaa gggaacaact ggaatttcta cctgaaatct cacctgatga
     8641 acaaaaaatt tatgacttgt ttagggaaga gcaacgcgaa ctctcttttg atgaactcct
     8701 gcttaagact ggctatagct ttggaaaatt atctactatc ctgctcaatc tggaattaaa
     8761 aggcttaatt atcaaatcag gaggaaacgc ttttctttta agctaagctc catagaatta
     8821 taaaaggatg caagatgtcc ccatcttgca aagcaccagg tttttcctaa ttaaagattc
     8881 atctatttat gtaatcctaa cctcaattat attatcctat tactgcactt aacattttcc
     8941 ctttcaaaaa attatctcag tcctacctct attctataat ataaagattt ctcctctctt
     9001 atttttccct aaattttttg tcaggtttct ctcctttatc atacatatca atttttagaa
     9061 ttaggatttt atctcaaagg atacaatacc taataaaaat atatttttaa ggagaaaaca
     9121 atgaaagaga ttattattaa tgccgaacag cttgaacaga agctacccaa aacttctcgt
     9181 tttcaccaat atcggatagc tttcaattct gcttttttgt atgaagattt tgctactgca
     9241 tctaaagtat gtgaagaaat ttcagctcta cctgaattga cagttaacga aagatgtgag
     9301 ttagcaagct attttcagat gttaggtgaa attgatgctg ctttaaagca acttgatctt
     9361 gtcctgcaag ctaatccaga ggaacataat gcggctcttc agaaagcaag aattttacga
     9421 cttcatcagg attctgaaaa atacctctcc tttatccaat ccagaattat agatttcccc
     9481 tctttcgctc cttattatct ggatgtatgg gaattcctaa aaactaatcc caataatgag
     9541 cttcgtgagc taatccagca acttgctcaa gaaaatagta ttgtccttcc cgaagaaaaa
     9601 gaggaattca tcctttctcc agaaagttcc actccttttt cgcaaacaga tatctattct
     9661 gattcagatt tactcacaat gataaatctt tttcagggta gagaaaatag ctatgctcgt
     9721 caatgggtct ccgatgaagg gaaatatggt tacactcctg ttaatgaacc tctgaatctg
     9781 aatgctgtcc gtaatcatct tcttggaatt caaactctgg gcatttatca actggattta
     9841 atgggaaaag tgaaatggat tgtctttgac ctggatgtag aaaaatctca tctggataat
     9901 ttgcatgacc cggcttttcg tctttggctg gaacaagggt ttcaaaagat tatcaatgaa
     9961 ttccgtgaat tactcaaaac ctatcatctg gaaatgaatg tggaattcag tggctataaa
    10021 ggttatcatc tctggattct tcttaaagaa aaaatccctg cttatgtagc tcgtagtttt
    10081 gctcagagaa ttgttgccca gataactttt aatggacttc ctatcagcat tgaaattttc
    10141 cctaaacaag taagaacttc ccgggacaac tatggcaatc tggtcaaact tccttatgga
    10201 attcatcgtc ttagcggact caattctact atgctcaatg aggaatttaa aatcatcagt
    10261 tttgaagagt ttatcaagca gccaaaaatg gtatctccag aagacctcat ttctgctctg
    10321 tttgctctgg accccaactt cagtctgatt caggaaaaag aaaatgccca agaacctgtc
    10381 tctcccttaa tctatcctga aaccctggaa gctcctgacc cagaaactga ttctgaatgg
    10441 ctgtgcttga aacaaaattg tgctgtgctc tggactattg ataatctgat aagtactaat
    10501 cattcactca caggtgaaca aaaaacagtt ttgcgctatg tctgtggaca tctcactaat
    10561 ggacccgcta tagtgaacac tcttatgaaa aagctgacca attgtgaact tgatgaccta
    10621 atgaagcgac cttttagggg aaatgtaatg agttgtaacc gtattcgctc tttagttgga
    10681 acgcttgtag aacctgagcg ctgtcattgc gatttctctc aagaatctgg aatgtatccc
    10741 actcctttat tgcatctaaa aaaactcagt tccaaatcca ttaatgctct ccaggctaat
    10801 gatttaatgc taaaagacct catctctacc tatctgaaac ttaaaagcca aaccagagat
    10861 ttacaggaac gattggaaca ggtagaaaaa gatattatta acgcatttga aaacattggt
    10921 ttagatgaaa tctccactcc ctatggtatc ttgaaaaaga taactaatgg ggaacagatt
    10981 tctctggtga tgcaacttaa ataaacggga ggtataaaat gaataataca gaattcttaa
    11041 atgaactgga acaagccaag caactggctc gtagtggtga actggatata gctctgcaga
    11101 tttttgaaaa gctttatgcc gctgataaca atgagcaaaa tctatctctc atctattata
    11161 tgcagaatag ccttaaacaa gcagctaatt atagtaaagc tatagagata ggactcgttg
    11221 cttgcgaact tccagaagtc tgggatagca ttcgtcataa taccgcctgg tgcatttact
    11281 ttcaatattt taaagagaaa tgtgaactcc ctgttgaaga agctatagaa tggctggaaa
    11341 aaataaaaga acttttccct cctgctcctg gtttgcatcc cttacctcta agtgtgtttt
    11401 cttacatcaa taagcatcca gaattatcac ctgctttaat tatccagctc tgttcccttc
    11461 tggaccctca aaaactggaa atggaacctg tccctgctaa agatggtaaa tactctctcc
    11521 cttctcaacg agaaaagtat attgacctcc tttctaaagc ttatattgcc gctggtaatt
    11581 acaatgattg cctggagcta tgtcagcaaa ctctgaatga acccataacc cttactccag
    11641 caaaacatat ctggctaaag cgtagaattg caatctgcca ttcacatctc ggagaagaag
    11701 aaaaagcact gaatgaaatg aaagaaattc tactcagtaa aaaagattgg ttcctcttct
    11761 atgaagctgc gcaaattgcc tttaacctga aaaacattga tgaagcagta gactttgcca
    11821 ttagagctgc cttagctcca ggtgaaattg aaaacaaaat acacctctgg gaattgctca
    11881 agtctatcct tttccagtta aaactttttg ataaagctat tgaaatgcta cagcttattg
    11941 cttctatccg aaaattcaaa caatggtcta tcccacaaag cctgaacaaa gaactgaatt
    12001 cctatcacat ctctctggat aatcttcagc attataaaga actcttttct caaatccgaa
    12061 aatggcttcc tgagttgtta actaatgatg cagaagaact ttctggaact atcaatttac
    12121 tgcttccgca taaaaaagct ggatttatct cctgtggaaa ggaatcctat tatttcagaa
    12181 ctgccgattg ccagattcct gtagatgaac ttcagcacgg tctaaaagtc aaatttatac
    12241 tccagcctgg ctttgacccc aaaaaacaaa aagagaccat cgttgccgtt aatattcatc
    12301 gggagtaaat atgtctgtcg tatatgtagc tgatcagggt gcttctatct gtaaacgcgg
    12361 agataggtta tatgtcttca aaggacctaa ccttctccgc tggtttcata gtaaggacat
    12421 cactcagctc attatagtag gtaatgtcac tcttactact cccgttctta cttatctgct
    12481 taaatataaa atagataccg tattcctttc ctactatgga aaatataaag gaagattgat
    12541 aggagaattt ggaaaaaatg tccagcttcg tattgctcaa tttcagttcc ttaattctct
    12601 tcaaaatcgt gaaagtcttg ctgctttgta tattcaggga aaattaaata atatgcaaac
    12661 ccatcttatg catcgtttaa aaagaaaaaa ggataaactc atctcaactg ctatcctgca
    12721 aaaccaggct atactggata aaattgaaca atcatctcct aatctggata cccttcgtgg
    12781 ctatgaagga attgcttcaa aaaactattt ctctgctttc ccagccttaa tttctaatcc
    12841 tcaattcatc tttacgggaa gaaatcggcg tcctcctaag gatgaagtta atgcactact
    12901 ctctttagga tatactttcc ttatgaatca agttatggga gcaacctata tctctggact
    12961 ggacccctat tatggtgctc tacacgacat agcttatgga agacaatccc ttgctctgga
    13021 ccttatggaa gaattccgtc c
//
LOCUS       DAORZF010000046        12326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_3999_length_12326_cov_371.188412, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000046 DAORZF010000000
VERSION     DAORZF010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 12326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12326
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3999_length_12326_cov_371.188412"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            262..828
                     /locus_tag="PK015_05935"
     CDS             262..828
                     /locus_tag="PK015_05935"
                     /inference="COORDINATES: protein motif:HMM:NF018410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HOL34042.1"
                     /translation="MNSRTKILISLMILIFCGSMLWGESYGEKTAANLKAKGWNPSLI
                     VILISTLPLVELRGSIPVAIAVLGMPWQKAVALSILGNMLPIPFLLLLLEWFFKMISR
                     YKWGKKFTDWMYNRTRNKGKVVERYEAIGLAIYVGIPLPGTGGWTGSLAAKIFGLPFT
                     KSLLWIFIGVLIAATLVTLLTLLGVAIF"
     gene            831..1988
                     /gene="lpxB"
                     /locus_tag="PK015_05940"
     CDS             831..1988
                     /gene="lpxB"
                     /locus_tag="PK015_05940"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOL34043.1"
                     /translation="MSNLTKDSYTIFWLAGENSGDLHSSIVMKSLNSGIPGVRHIGIG
                     GPRMQAEGLKPLFPFAPFNVMGFVEVAGHLGFFWKVERQLKKFFREEKPDLAILVDYP
                     GFNLRIAKMADDLHIPVLFYICPQFWAWKHNRVYKLKENVRQVACILPFEKELLEMHN
                     ISATYVGHPIAEEIKFELDREGFARFYGINPEKQWIGFLPGSRDNEVKRMLPAFLEAA
                     KILDENRFEIMISKASTVSHSLFMHICESSGLPRLHIVDGYRYEMMKYADYLVSTSGT
                     ATLEAAFIGTPLTICYKAAPISYLIGRNLVRIKRIGLPNIVLDKDLLPELIQKDCNPR
                     NIVKTVLETLDNPDKINLIKKELSQLKALLGDRKTSIEMLNLVKKMLETYA"
     gene            1981..2592
                     /locus_tag="PK015_05945"
     CDS             1981..2592
                     /locus_tag="PK015_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOL34044.1"
                     /translation="MPEFLMKVEAVLGAGLLKLLRATLRIAVHNQPSDDYRCVYAFLH
                     RNLLLLTLHRINSQIAVMVSSSKDGELIAGPLKYLGYIPIRGSSTHQGSQALKEMVRL
                     SRTHSLAITPDGPKGPLGTIHPGLFQLAYLAKIPIIPVSANSSKEWLVKSWDRFRIPK
                     PFSKLLVKYGEPIWVNNKEDFLEAEKALRESWKQLDLTLSSPK"
     gene            complement(2642..3385)
                     /locus_tag="PK015_05950"
     CDS             complement(2642..3385)
                     /locus_tag="PK015_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34045.1"
                     /translation="MRKYFIILIIALMAVGLFLTACEEDENITEPENAKWSVFVTANP
                     PESQHSHSVTVMWIGPAQDYVEPSSIKLEIGGDEVNLEKWAGIWIGEKNLTPGEKYSF
                     KLKVDDETIVNTNQKIVYNADATFPETYNPELTAYVEWTLEKDNEHQIASAYALDPED
                     AANDEVVDEELEPSARSYQFPAGVVSYHGEGTIYTLEISQMNYKYVDNVMVASSQADY
                     QDYGNIKRDRGSREETVKYVKHMLGIIMK"
     gene            complement(3416..4270)
                     /locus_tag="PK015_05955"
     CDS             complement(3416..4270)
                     /locus_tag="PK015_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34046.1"
                     /translation="MKTKSVLIIMAVAVLIIMTACSGVPKEKKLTVSNVNVIGDAKDL
                     IKVADGTYTLQNANNELSLTINLQLLQNRDLEMEGLKLGQWTLNLLDKDGNSIPGAML
                     QFKPESGAELQIIEFLKNGMIDDTLSVTFSQMVTSQDELKKIMKQTTSFELTTVIESI
                     MPGPLAEVTPEETKPTKPSTTQPAQKPQAGQTTPQTTPETSPTATPVKPSEDWDKELD
                     AYSAAIDRYISMKNNLKPGDANAIRRLNEQKAKIDEMTKRFQTASGMTAAQSTRFKNL
                     KAKFDNAG"
     gene            complement(4792..>5136)
                     /locus_tag="PK015_05960"
     CDS             complement(4792..>5136)
                     /locus_tag="PK015_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOL34047.1"
                     /translation="LIWGFINLFITVKITDLTAEGLPYVKGVYVISPEIETIKEEIFR
                     QLDRGVTFFKARSGYENKDIEVLFCVLNRRQVGQLTDIVKDSDPDAFMIVTDVYDVMG
                     YGFRSRNLDLSE"
     assembly_gap    5137..5236
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5237..6790
                     /locus_tag="PK015_05965"
     CDS             <5237..6790
                     /locus_tag="PK015_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOL34048.1"
                     /translation="ILSFKIDHTQYVNEEFIPQHSMMIDCITFNHPEGSRIYQDSAIF
                     ILAKAVNSLLGIQHSLVVEHSIGDGVFCEVFGAQRWTEQDCNRVKEEMIKIVESDLPI
                     DKITVPISEALEIFSTMGRKDVIKNLKYNYRDEVSIYRCGKYYDIFLRPLADRTGRIK
                     EFDIVYQEPGFILRFPTGKECKLAQPFVFPKKLFQLHQEHDKWLDILGVHNIIDVNKL
                     TDNYDISEFILVEEALHEKKIAEIAAAIVSEPKIKLILIAGPSSSGKTTFAKRLSIQL
                     LACKTKPVMISLDDYFIDREKTVRKENGEYDFESIYALDLDFLNLQLTQLLAGQEIEL
                     PHYDFTRGIRRRSNVIVKLRENDVIVIEGIHGLNELLTSSIKAENKVKIYVSALNQLN
                     IDNHNRIATTDCRLIRRIIRDYQYRGYTAIETIQRWPSVREGEEKNIFPYQENADFMF
                     NSSLTYELGVLRKYAWKLLLSVPKSSAGYTEAQRLLALLSNIKDIPDSLVPYNSIIRE
                     FTNGSIFRY"
     gene            6793..8691
                     /gene="mutL"
                     /locus_tag="PK015_05970"
     CDS             6793..8691
                     /gene="mutL"
                     /locus_tag="PK015_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOL34049.1"
                     /translation="MNRIHILSEDVSNKIAAGEVIERPVSVVKELVENAIDAGATIIT
                     VSIENGGKELIQVSDNGSGMSADDALLAFERHSTSKIKTVDDITHIETLGFRGEALPS
                     IASVSNLTLITKDADSEVATQIEYQFGRLVNVNQISSNQGTTVSVKAIFKELPARRKF
                     LRSSQTELHHILRYLHYQAILNPALGIKLYVDGNQRLSFVPAQNREERMGEVFGSGFF
                     NDDIIPINIENEGYKLEGYIFGLEDRSDKLIDMQYIFINGRFIPDKTVKHAIKSAYEP
                     FILKTRAWQKGNIPPYILFLVVPADQIDVNVHPAKMEVRFREQQKVHSLIFTTLTEAL
                     NNYEYEKFATARAKFQDLSSSQPVTSTERAIFTSNTHIPRFSEYKKEYGKLFQPEIFP
                     QNDEKPAETKEIPLVNLDKKQEEEQLEILPDLPNDSFHYRLLLSNEEDYINPWQLHNT
                     YIFIQVEDGFVIIDQHAAHERIIYEKLLQRTQGAPAVRQKLLIPLVIDIPPIIATEIR
                     ELIEQNLELLEKTGFVLKKFSGDSLVIEEIPAELDDWQGGKVFIDILKQLQDELETNS
                     DFRDSLAKSIACKAAIKAGKKLTRKEMLNLINQLFACNMPYFCPHGRPLIVKVTLNDL
                     EKKFKRQL"
     gene            8688..9608
                     /gene="miaA"
                     /locus_tag="PK015_05975"
     CDS             8688..9608
                     /gene="miaA"
                     /locus_tag="PK015_05975"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOL34050.1"
                     /translation="MIPLITLEGPTASGKTELAILLAKELGSEIISADSRQVYRYLDI
                     GTAKPSVEQLQQAKHHLISIIDPSESYNAGRFCADAGNIIEDLNSRGIIPIVCGGTGL
                     YVSSLLTGLFPEIKMDKDIRANLIKRMNQEGLASLFEELKKIDPDFAFRISSNDKQRI
                     LRGLEVYYATGIPITKHWKNQTSHNRYHTFRILLQPPREILYPRINNRIQQMIDSGLL
                     NEIEHLFKLGYTPLSPGLNTLGYKEFLPYFLESAPLNQCISLAAQHTRNYAKRQYTWY
                     RKYKFDLTFTSNDYIISEIITIIKTYFKKK"
     gene            9619..10446
                     /locus_tag="PK015_05980"
     CDS             9619..10446
                     /locus_tag="PK015_05980"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOL34051.1"
                     /translation="MHLIARVFDVEITEEELLQESVTLYGQDSLNGLKRALDMLINRY
                     LLYHQALLNGFEVSEEEFDNALLEALDFDDSVANNEHTPSQIEEQIKQCILIRKYMQQ
                     IYPSDGEIPESELLAFYEEQEENFSSQEMVRASHILIRKDEEDAEEKAKKLREKIHTP
                     ADFKNVCVQYSQCPSGVYGGDLGWFPRGKMIKEIEDVAFSLQPNEISEVFPTKYGYHI
                     LMVTDRREPHPIPFEDIRESLGTSLNEFRKEFVLDRHIQELRNTYKDQIEILDENYL"
     gene            complement(10450..11196)
                     /locus_tag="PK015_05985"
     CDS             complement(10450..11196)
                     /locus_tag="PK015_05985"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOL34052.1"
                     /translation="MQPILRAEHLYKSFLLAGKPQLILEDVNFTMEEEGFVSVVGSSG
                     CGKTTFLELLAGITKPDKGEIYYQGERITGKTGLLGYMPQDDLLFPWLNVQENVLIPV
                     RVRNGDIKAAKARIQELLPVFGLEGHIKHMPYQLSGGLRQRAAFLRTCMMEAKLLLLD
                     EPFASLDAITRIQLQNWLGYIARELKLSIILVTHDIEEAINLSDTIMVMRKQPGKFVC
                     SFKLDRSNKMNEMEVLKLKKEILELVANGV"
     gene            complement(11174..12178)
                     /locus_tag="PK015_05990"
     CDS             complement(11174..12178)
                     /locus_tag="PK015_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOL34053.1"
                     /translation="MKRQLMILTVITAILLFGVTACKKKAKPETTLRKVRVVLDWTPN
                     TNHSGLYVAQGLGYYKEKGLEVEILQPGENTVEKIIASDQAEFGVSYQESVTIARSEN
                     IPVKSIAAIIQHNTSGFASLKSANIISPKDFEGKRYGSSGWPSELEILKQVMESYNAD
                     YKKVQIVYGITDFFSTIGRDADFAWIYYGWDGVQAKLRGIELNYIPVRDLDPVFDFYT
                     PVLIANDQLLNSDPELVRDFLEATSKGYEYCVQNPESAAEIFWKAVPELDKNLVKASL
                     NYLKTEFISDASQWGLQDFSVWQRFADWMYQKHIIQKSLKAQELFTNEFLPNATHTKS
                     "
BASE COUNT         3772 a         2330 c         2230 g         3894 t
ORIGIN      
        1 tatgtgtaag ttatgtccgt tttcggacat aagctggaca tcagttggac ataagctgga
       61 cataagctac tcatcatttg gattgcaatt cgcttatttt tggataattt aaattatgga
      121 attgttagat attcttcagg gttattcaag agcattaata aattttagta ttcggatatg
      181 catcgttacc tatagatttc tcttgtcaaa ataattattg gttagaattt gttttttcat
      241 aaaagataaa atggagaagc ggtgaattcc agaaccaaga tattgataag tttaatgata
      301 cttatttttt gtggaagtat gctctggggt gaaagttatg gtgaaaaaac agctgctaac
      361 cttaaggcaa agggttggaa tccttcacta atagtgatac tcatttcaac tttaccatta
      421 gtggagctaa gaggttccat acctgtggca attgcggttt tagggatgcc ctggcaaaag
      481 gcagtagctc tttccattct gggcaatatg cttcctattc cttttttact tcttttatta
      541 gaatggtttt tcaagatgat ttcaagatac aaatggggta aaaaatttac cgattggatg
      601 tataatcgta cgcggaacaa aggtaaagta gtagagcgat atgaggcaat tggtttagca
      661 atatatgtgg gaataccttt gcccggcacg ggaggatgga caggttctct ggcagctaaa
      721 atctttggtc ttccctttac taaatcgcta ctctggattt ttattggagt gctaatcgct
      781 gctaccctgg tcacattatt aactctttta ggagtagcta ttttttaact atgtcaaact
      841 taactaagga ttcctacact attttctggc tggctggaga aaattctggt gaccttcatt
      901 ccagcattgt gatgaaaagt ctcaattctg gcattccagg cgtccggcat ataggaatag
      961 gtggacctcg tatgcaagca gaaggtttga aacctctctt cccttttgct ccttttaatg
     1021 ttatgggatt tgtagaagtg gctggacatc tgggtttttt ctggaaggta gagcgacagt
     1081 taaaaaagtt tttccgggaa gaaaagcctg atcttgctat cctggttgat tatccaggat
     1141 ttaatcttcg gattgccaag atggctgatg acctgcatat ccctgtgcta ttttatatct
     1201 gtcctcagtt ttgggcttgg aaacataatc gggtctataa attgaaagaa aatgttcgcc
     1261 aggtagcctg tattttacct tttgaaaaag aattgctgga aatgcacaat atatctgcaa
     1321 cttatgtggg acatcccata gctgaagaaa taaagtttga acttgataga gagggttttg
     1381 cccgtttcta tggtataaat ccagaaaaac aatggattgg ttttcttcct ggtagtagag
     1441 ataatgaagt aaaaaggatg cttcctgctt tcctggaagc agcaaagata ctggatgaaa
     1501 atcgctttga aattatgata tccaaggctt caacggtttc tcattcactt tttatgcata
     1561 tatgtgaaag ttcaggatta ccccggctac atattgtaga tggttatcgt tatgaaatga
     1621 tgaagtatgc agattatctg gttagcactt ctggaacagc aacactggaa gcagcattca
     1681 ttggtactcc tttaactatc tgctataagg cagctccaat ttcctatcta ataggtcgta
     1741 acttagtgcg aattaaaaga ataggcttgc caaacattgt cttggataaa gaccttttgc
     1801 ccgaattaat ccagaaagat tgtaatccca gaaatattgt taaaaccgtg ttagagaccc
     1861 ttgataatcc tgataaaata aacttaatta aaaaggaact ttctcaatta aaagcactac
     1921 tgggagaccg caagaccagc attgagatgc tcaatctggt taaaaaaatg ttagaaactt
     1981 atgcctgaat tccttatgaa ggtagaagca gtccttggtg ctggattatt aaaactttta
     2041 cgagctacat taaggatagc tgttcacaat caaccttcgg atgattatcg ttgtgtttat
     2101 gccttcctac atcgcaatct tttgctccta accttacaca gaatcaattc acagatagca
     2161 gtaatggtct cttcttcaaa ggatggagaa cttattgctg gtccacttaa atatttaggc
     2221 tacataccta ttagaggttc ttccacacat cagggttctc aagcactaaa agaaatggtt
     2281 cgtctatcaa gaacacacag cttagcaatt acacctgatg gacctaaggg tccactcgga
     2341 accattcatc ctggtctgtt tcaacttgct tatttagcca aaatacccat aattcctgta
     2401 tctgctaatt ccagtaaaga atggttagtc aaatcctggg atcgttttcg catacccaaa
     2461 cccttttcta agctattggt aaaatatgga gaacctatct gggtaaataa taaagaagac
     2521 tttctggaag ctgaaaaagc acttcgtgaa tcctggaaac aattggattt aactctttca
     2581 tctcctaaat aaaaaaagcc ggaacaagtt cgctccggct ttgtattgtt agaattaaag
     2641 gttacttcat tattatgcca agcatatgct tcacatattt aactgtttct tcccgagaac
     2701 cacggtctct cttaatattg ccataatcct gataatctgc ttgagaggaa gctaccataa
     2761 cattatccac atacttgtag ttcatctggc tgatttctaa ggtatagata gtgccttcac
     2821 catgataact aactactcca gcagggaatt gataggaacg agcagagggc tccagctctt
     2881 catccactac ttcatcatta gctgcatctt cagggtctaa agcataagca gaggcaattt
     2941 gatgctcgtt atccttttcc agtgtccatt ctacataagc agttaattcg ggattatagg
     3001 tttcagggaa agtggcatca gcattataaa caatcttctg attggtgttt acaatggtct
     3061 catcatcaac ttttaattta aagctatatt tttctccagg agttaagttc ttctccccta
     3121 tccaaatacc agcccatttt tccaaattaa cctcatctcc gccaatttcc agtttaatgg
     3181 aagaaggttc tacataatct tgagcaggac caatccacat cacagtcaca gagtgtgagt
     3241 gctgactttc tggaggattg gcagtaacaa aaactgacca tttagcattt tctggttcag
     3301 taatgttttc atcctcttca caggcagtta agaataatcc cactgccata agagcgataa
     3361 ttagtataat aaaatattta cgcatcgtaa tctcctttct ttaaaattat ttaaattaac
     3421 cagcattatc aaacttggct ttaagattct taaatctggt ggattgagca gctgtcatac
     3481 cagaagcagt ctggaatctc ttagtcatct catcaatttt agctttctgc tcattgagtc
     3541 ttcttatagc attggcatcg ccaggtttaa ggttgttctt catagagata tagcgatcta
     3601 tggcagcact atatgcatca agttctttat cccaatcctc tgaaggttta actggtgtag
     3661 cagttggaga agtttctgga gttgtttgag gagtagtttg acctgcttgt ggtttttgag
     3721 ctggttgagt tgtggaaggt tttgttggtt ttgtctcttc tggagttact tccgccaaag
     3781 gaccaggcat aatactttca atcacagtgg taagttcaaa agaagtagtt tgtttcataa
     3841 tcttcttaag ttcatcttgg gaagtaacca tctgactgaa agtaactgag agtgtatcat
     3901 caatcatacc atttttgaga aattcaataa tctgtaattc agcgccactt tctggtttga
     3961 actgaagcat agcaccggga atggaattac catctttatc cagaagattc aaggtccatt
     4021 gacctaattt taacccttcc atctccaaat ccctattttg taacagttga aggttaatag
     4081 tcaaggatag ttcgttgttg gcattttgaa gagtgtaagt tccatcagca actttaatta
     4141 aatccttggc atcaccaata acattaacat tactaacggt aagttttttc tcttttggaa
     4201 caccgctgca ggcagtcata ataattagca ctgcaacagc cataataatc agtaccgatt
     4261 tagtcttcat ctttttctcc tttttccttt agttttggac attattattt gcttagcttc
     4321 ttttagaaaa tttattttca aaacatacct cccttttata ttataataac tttctaaaaa
     4381 atccaagcaa gtatatattt aaacccaagt actatatctt caattctgtc aatgtttttt
     4441 tcttttattc agtcgttttc aaatcttttt ttagcaaatg aaaggtatga gtgtttaaaa
     4501 atctcacgca gataacgcag ataaatctgt gttaataaat tcatcatttt gagagggtaa
     4561 tggttgttta ctaagtaagt tgctatttta tccttgtcat taaatagctt caaatgagaa
     4621 gattgtaacc ttataaaaaa cggcatataa aatcattcct tatctttatc tcttgtaatg
     4681 gaacaagagt ttatttttaa aaagttcaga caaacaacag attctgctct tagccttgaa
     4741 atatagggtt ataaaatcat aattagtcag aaggctgacc attttctatt ttcattcgct
     4801 aaggtccaga ttacgagagc ggaatccata tcccataaca tcatagacat ctgtaacaat
     4861 cataaaggca tcgggatcgg aatctttcac aatatcggtc aattgtccaa cctgacgacg
     4921 attcaataca cagaataaga cttcaatatc cttgttttca taaccacttc tggctttgaa
     4981 gaaggtaact cctctatcca gttgtctaaa tatttcctct ttaattgttt ctatctcagg
     5041 agagatcaca tacacacctt taacataggg taatccttca gcagtcaaat ccgttatctt
     5101 gacagtaata aagaggttaa taaatcccca gataagnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnattc tatctttcaa aattgaccat actcaatatg ttaatgaaga
     5281 atttattcca caacacagta tgatgattga ttgtatcact tttaatcatc ctgagggaag
     5341 tcgtatctat caggattcag ctatatttat actggctaaa gcagtaaatt cactacttgg
     5401 aattcaacat tcattggtag tggaacattc cattggtgat ggagtatttt gcgaagtttt
     5461 tggtgctcaa cgctggacag aacaggattg taatagagtg aaagaagaga tgattaagat
     5521 agtggaatcc gatcttccca ttgataaaat aactgtcccc atctctgaag cattagaaat
     5581 attcagcact atgggacgaa aagatgtgat aaaaaacctg aaatataact atcgggatga
     5641 agtctctatc tatcgttgtg ggaagtatta tgatatattt ttaagacctc tggctgatcg
     5701 cacaggtagg attaaggaat ttgatatagt ttatcaggaa cctggtttca ttcttcgctt
     5761 tcctacgggt aaagaatgta aattagccca gccttttgtc tttcccaaaa aactattcca
     5821 attacaccag gaacatgaca agtggttgga tattcttggg gtgcataata tcattgatgt
     5881 taataagctc acagataact atgatatctc agaatttatt ctggtggaag aagctctgca
     5941 tgagaagaaa atagctgaaa ttgctgctgc tatcgtaagt gaacctaaaa tcaaacttat
     6001 ccttattgca ggaccttctt cttctggtaa aacaaccttc gctaaaagac tttccattca
     6061 gcttcttgcc tgtaaaacga aaccagtaat gataagtctg gatgattatt tcatagatag
     6121 agagaagacc gtccggaaag aaaatggtga atatgatttt gaaagcatct atgctttgga
     6181 cctggatttt ctaaatcttc agctaactca actacttgct ggtcaagaga tagaattgcc
     6241 tcattatgat ttcacccgag gtatccgaag aagaagcaat gttatcgtta agctgagaga
     6301 aaatgatgtc atagttattg aaggaataca tggtttaaat gagcttctga cctcttctat
     6361 caaagcagaa aataaggtta aaatctatgt aagtgcactg aatcagctca atatagataa
     6421 tcacaatagg atagccacta cagattgccg tttaatccgt cgtattatta gggattatca
     6481 ataccgaggt tatactgcca tagaaaccat tcagcgctgg cccagtgtgc gtgaaggtga
     6541 agaaaaaaat atctttccct atcaggaaaa tgctgatttt atgttcaata gcagtctcac
     6601 ttatgagctg ggagttttac gtaagtatgc ctggaagctt ctgctttctg taccaaaaag
     6661 ttctgctggc tacacggaag cacaaagatt actggctctt ctttccaata ttaaagatat
     6721 tcctgatagc ctggttcctt ataactctat cattagagaa tttaccaatg gctcaatttt
     6781 ccgctattaa ttatgaatag aatccatata ttatccgaag atgtgagcaa caagattgct
     6841 gcaggagaag tgatagaacg acctgtttct gtagtaaaag aactggtgga aaatgctata
     6901 gatgctggtg ctacaataat tacagttagc atagaaaatg gaggaaaaga gctgattcag
     6961 gttagtgata atggctctgg tatgagtgct gatgatgctc ttctcgcttt tgaacgacat
     7021 tccaccagca agataaagac cgttgatgac ataactcaca ttgagacctt aggttttcgg
     7081 ggagaagcac ttcccagtat tgccagtgtc agtaatctta ctcttattac caaagatgct
     7141 gattcagaag tggcaactca gatagaatat cagtttggaa gactggttaa tgttaatcag
     7201 atttcatcca atcaaggaac cactgtctcg gtgaaagcga tatttaaaga gcttcctgca
     7261 agaagaaagt tcctccgtag ttctcaaacc gaattgcatc atattctgcg ttatttgcat
     7321 tatcaggcaa ttctgaatcc tgctttagga attaagctat atgtggatgg caatcaaaga
     7381 ctttcctttg tccctgctca gaacagagaa gaaagaatgg gtgaagtgtt tggctcaggt
     7441 ttcttcaacg atgatattat ccctataaat attgagaatg aaggctataa actggaaggt
     7501 tatatctttg gattggaaga tagaagcgat aaacttattg atatgcaata catctttatc
     7561 aatggacgct ttattcctga taagaccgtc aaacatgcca ttaaatctgc ctacgaaccc
     7621 ttcattctta agaccagagc ctggcaaaaa ggaaacattc ctccctatat tcttttctta
     7681 gttgttcctg ctgaccagat tgatgttaat gtccatccag ctaagatgga agtgcgtttc
     7741 cgcgaacaac aaaaggtgca ctctttaatc tttaccactc tgaccgaagc tttgaataac
     7801 tatgaatacg aaaagtttgc cactgccagg gcaaaatttc aggatttatc ttccagccaa
     7861 cctgttacca gcacagaaag agctattttt accagtaata ctcacatccc tcgtttttcg
     7921 gaatataaaa aagagtatgg caaactcttt caacctgaga tattcccaca aaacgatgaa
     7981 aagcctgctg aaaccaaaga aatccctctg gttaatctgg ataaaaaaca ggaagaagaa
     8041 cagctggaaa tcttacctga tttgcctaat gattcttttc actatcgttt gctgcttagt
     8101 aacgaagaag actacatcaa tccctggcaa ttacataata cttatatatt catccaggtt
     8161 gaagacggat ttgtaatcat agaccagcat gcagctcatg agagaattat ctatgaaaag
     8221 ttactacaaa gaactcaagg agctcctgct gttcgtcaaa aactccttat tcctttagtc
     8281 attgatatac ctcccattat agctacagaa attcgtgaat tgatagagca aaatctggaa
     8341 ttactggaaa aaactggttt tgtcctcaag aaattcagtg gagactcact cgtaatagaa
     8401 gaaattccag cagaactgga tgactggcaa ggcggaaaag tcttcataga tatattgaaa
     8461 cagcttcagg atgagctgga aactaactca gatttccgtg attctctggc taaatctatt
     8521 gcctgcaaag cagctataaa agcaggaaaa aagcttactc gcaaagagat gttgaacctc
     8581 attaatcaac tttttgcctg taatatgccc tatttctgtc ctcatggcag acccttaatc
     8641 gtaaaagtta ctcttaacga tttagaaaag aaatttaaac gccagttatg attcctctta
     8701 tcactctgga gggaccaact gcttcgggca agacagaact ggctatcctt ttagctaaag
     8761 aacttggttc tgaaattatc tccgctgatt ctcgtcaggt ttatcgctat ttagatatcg
     8821 gaactgctaa accttcagtt gaacaattac agcaggcgaa acatcatcta atcagtataa
     8881 ttgacccttc agaaagttat aatgccggca gattttgtgc tgatgcagga aacatcattg
     8941 aagacttgaa ttctcggggt attataccca tcgtttgtgg tggaactggt ctttatgtgt
     9001 cttctctatt aactggattg ttccctgaaa ttaagatgga taaagatatc cgagccaatt
     9061 taataaaacg gatgaatcag gaaggtttag cctccctttt tgaagaactt aaaaagatag
     9121 accctgattt tgcttttaga attagctcta atgataagca acgcatactg cgtgggttag
     9181 aagtttatta cgctaccggc attcctatta ccaaacactg gaaaaatcag accagccata
     9241 atcgctatca tacttttcgt attttgcttc aacctcctcg tgaaattctt tatcccagaa
     9301 tcaataaccg cattcaacaa atgatagata gtggcttatt aaatgagata gagcatctat
     9361 ttaagctcgg atatactcct ctttcacctg ggttgaatac cttaggttat aaagaatttc
     9421 taccctattt tctggaaagt gcacctctga accaatgtat ttctctggct gcacaacata
     9481 ctcgtaatta tgccaaaaga caatatacct ggtatcgtaa atacaagttt gatttgacat
     9541 ttacctcaaa tgattatatt atatcagaga taatcactat aattaaaaca tattttaaga
     9601 aaaaataatg aggataaaat gcatctgata gccagagtat ttgatgtgga gattacagaa
     9661 gaagaacttc tccaggaaag tgttactctt tatggtcagg attccttgaa tggattaaaa
     9721 cgcgctttag acatgctgat aaatcgttat ctgctctatc atcaagccct gttaaatggt
     9781 tttgaggtct cggaggaaga atttgataat gccctcttag aagcactgga ttttgatgat
     9841 tcagtagcta ataacgaaca tacaccttcc caaattgaag aacagattaa acaatgtata
     9901 ctgattcgta agtatatgca gcagatttat ccatctgatg gagaaatccc tgaaagtgaa
     9961 cttctggctt tttatgaaga acaagaggaa aacttttcct ctcaggaaat ggtgcgagct
    10021 tctcatatcc ttattcgcaa agacgaagaa gatgcagaag agaaagcaaa gaaattacga
    10081 gaaaaaatcc atactccagc agactttaaa aatgtctgtg tccagtattc acaatgtcca
    10141 tcaggagttt atggtggaga cttaggttgg tttcctcgtg gcaaaatgat aaaagagatt
    10201 gaagatgtgg ctttttcttt acagcccaat gaaatcagtg aggtcttccc tactaaatat
    10261 ggttatcata ttttgatggt tacagatcgt cgtgaacctc atcctatacc ctttgaagat
    10321 atccgggaaa gcttaggaac ttctctcaat gaatttagaa aggaattcgt cctggatcgt
    10381 catattcaag aactacgcaa tacctataaa gaccagatag aaatcctgga cgaaaattac
    10441 ctctaagtat cacactccat tagctaccag ttccaatatt tcttttttca atttaagcac
    10501 ttccatttca ttcattttat tggaacggtc taacttaaaa gaacatacaa atttccctgg
    10561 ttgctttctc atcaccataa tggtatctga tagattaatc gcttcttcaa tgtcatgcgt
    10621 gaccagtatt atgctcaatt ttaattctct cgctatataa cccagccagt tttgcaactg
    10681 gatacgagtg atagcatcca gactggcgaa aggttcatcc agcaagagaa gttttgcctc
    10741 catcatacag gtgcgtaaga aggctgctct ctgacgcaat cctcctgata actgataagg
    10801 catatgcttg atatgacctt ccaatccaaa gacaggtaat agttcctgaa ttctggcttt
    10861 agccgctttg atatcaccat tacgcactct aactggaata agaacatttt cctgaacatt
    10921 caaccagggg aaaagcaggt catcctgtgg catataccct aagagaccag tctttcctgt
    10981 aattcgttca ccctgataat aaatttcacc tttatcaggt ttagtgattc cagctaaaag
    11041 ttccagaaag gtagttttac cgcaaccact gcttcctacg acactcacaa atccctcctc
    11101 ttccatagta aagtttacat cttccaggat gagttgcggt ttacctgcca gtaagaatga
    11161 tttataaagg tgttcagctc ttagtatggg ttgcattggg taaaaattcg ttagtgaaaa
    11221 gttcctgtgc tttcagactt ttttgaatta tatgtttttg atacatccaa tctgcaaaac
    11281 gctgccagac cgagaaatct tgcagtcccc attgcgatgc gtctgaaata aattcagtct
    11341 ttaaatagtt tagactggct tttaccagat tcttatccag ttcaggcact gctttccaaa
    11401 aaatttccgc tgctgattca ggattttgaa cgcagtattc atagccttta ctggtagctt
    11461 caagaaaatc acgaaccaat tcagggtcag aattaagcaa ttggtcattg gcaattaaaa
    11521 ctggtgtata aaaatcaaaa actgggtcta aatcccgaac tggaatataa ttcaattcta
    11581 ttcctctaag cttagcttga acaccatccc agccataata aatccaggca aaatctgcat
    11641 ctctaccaat agtggaaaag aaatccgtta ttccataaac aatttgaact tttttataat
    11701 ctgcattgta agattccatc acctgtttca aaatttccag ttcagaaggc cagccagaac
    11761 tgccatatct ctttccttca aaatctttag gactgataat attggcactt tttaatgagg
    11821 caaaaccaga ggtattatgc tggataattg ccgcaataga tttcactgga atattctccg
    11881 aacgagcaat agtgacgctt tcttggtagc taactccaaa ttctgcctgg tcagaagcaa
    11941 taattttttc cacagtattc tctcccggtt gaagaatttc tacttccaga cctttctctt
    12001 tataatagcc aagtccctgt gcaacataga gtccactatg attggtattg ggtgtccagt
    12061 ccagaactac tcgcactttg cgtaaagtag tttctggctt tgcctttttc ttgcatgcgg
    12121 taactccaaa caataatatt gccgtaatta ctgtcagaat catcaattgc cttttcattt
    12181 ttgctcctgt atcttcatat tttataatta attacttatt tattttggct ctcacctttt
    12241 tagttcctaa aagctgttaa ttttatatac tatgttttta gctcttactt tatgtcaaat
    12301 cctttcagtt ttcttactac ttattt
//
LOCUS       DAORZF010000047        12082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_4082_length_12082_cov_369.303401, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000047 DAORZF010000000
VERSION     DAORZF010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 12082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12082
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4082_length_12082_cov_369.303401"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PK015_05995"
     CDS             <1..364
                     /locus_tag="PK015_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOL34054.1"
                     /translation="EKVTRELYEEFHMVLYPERITLKVNADELFKMADDAAKSRNFND
                     AIAIYDQIIQHFPNGSDDYRAFFMKAFIIAEELKDEERALQLFKDFLKKYPQGDLNES
                     AQFMIDALEGRIQLELEE"
     gene            866..2323
                     /locus_tag="PK015_06000"
     CDS             866..2323
                     /locus_tag="PK015_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HOL34055.1"
                     /translation="MEKLKTDLSNLEEYFNCNYNAEKSASAQTIFLKKLAELSHRYYH
                     GKMQTLPKVGIWNFNWFNVWYTPGVSSISTTIRDNPEESFELSNRGNLVAIVTDSTRV
                     LGDGDCGIYGGLGVMEGKAMLMKYLGGLDAISLCVNDRDAKGNPVPEKLIDFVKMLEP
                     SIGAVNLEDISQPNCYYVLDELLTGCEIPVWHDDAQGTACVTLAGLINALKLVDKKIF
                     DIKVVLYGAGAANTTIARFLMKEGLDPANLIMFDSKGALHSDRVDLANNPDKFRQWDF
                     AQKTNPKHYNTMEEAMKKADVLISLSTPGPDIIKPEWIKLMAEKPIVFACANPVPEIY
                     PYAAKSAGAFIVATGRGDFPNQINNSLGFPGILKGALMVRSRHITDTMAIKAAYALAT
                     FAEQRGISPDNIIPQMDEVDVFPYEAAEVASQAMQEGVARKTNSYEGLYQQARTDILQ
                     ARETCQKMIKDGSIPPIPEKLIEEALQHTNKMISK"
     gene            2325..3212
                     /locus_tag="PK015_06005"
     CDS             2325..3212
                     /locus_tag="PK015_06005"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HOL34056.1"
                     /translation="MQKQKKKNNLRGVLSFILKLGITILIMWSIFSKLNLKVVIREFV
                     TQPVWVILVLIGITVLRHWGQYLTWKWALQVNPNYHPAKYEIFNSYMIGQALRFAIPG
                     SFGMIGKIAFVHNDSKINSLYSFILERIFVTWVLLFFASMALVFVDLGIPSWISWLLF
                     LIFDTMPFWMYFLLSVDRRLKKLQSGYLKLAPAMVFAQIVITLLNYIQYWILLEQVQP
                     VSFWEIMNRMSLSHLAYSIPITFAGLGVKEKLAIPLLSDIGYLSENIVSTTLIIFIIH
                     DVAAALIGAVIFLRVKRSK"
     gene            3223..4521
                     /gene="trmFO"
                     /locus_tag="PK015_06010"
     CDS             3223..4521
                     /gene="trmFO"
                     /locus_tag="PK015_06010"
                     /inference="COORDINATES: protein motif:HMM:NF003739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HOL34057.1"
                     /translation="MPEIKIIGAGLAGSEAALYLADKGWKVKLYEMRPAKMTPAHQTG
                     YPAELVCSNSLKSTLLDTPAGLLKEELHLLGSKLLPVAESCAVSAGHSLAVDRNLFAQ
                     KINDEIIHHPNIEFIREEITTLPTELSILATGPLTSDNLIQELQSVVGSAHLYFYDAI
                     APIVSADSLNMDKIYLKDRYDKGEPDYLNCPFTEEEYYHFVEALLTGEKHPPHSFENE
                     MTEAKLKFYENCIPIEELARRGKDTLRFGVMRPIGLEKEGKTPFAVLQLRPENKEHTA
                     YNLVGCQTMLRYSEQKRIFRLIPGLEKAEFLRYGSLHRNSYLNAPAVLNPELNLKNAP
                     HIYVAGQLSGVEGYVECIASGLLVAKIISEGLAMLPPETITGQIWRRLIDTSIKNFQP
                     VNANYGLLPALNNPLRDKKQKKLALSERSIKALKDFLDNK"
     gene            4578..5906
                     /gene="hflX"
                     /locus_tag="PK015_06015"
     CDS             4578..5906
                     /gene="hflX"
                     /locus_tag="PK015_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOL34058.1"
                     /translation="MLDEEKDYLVEEFYNEPDTWEKGEEEEKTAFLATLATRGEKESE
                     TEASLNELGKLAETAGIAVLGKYTQKRENPESSTYFGKGFLEELSRKMHQNNADFLIV
                     NDELTPVQARNIENDYGIKVMDRTEIILSIFHQHAHTKEAKLQVRLAELQYQLPRLRR
                     QSENYDQEHGAVRSRGGSASRGMGEKQIEVDKRIIKDNIRKIKQAIAEISKNKETQRK
                     QREKSKKICLVGYTNAGKSTLFNALTEAGVLVEDKLFATLDSTTRQLKLSTSFPVVIS
                     DTVGFISRLPHQLVASFTATLMEVEDADLLLHIVDLSDERYEYYIEQVQSVLKEIGAD
                     NIPQILVFNKIDLVDSIQLSLAKKRYPDAIFISALNKSHLEDLLQKVEEKLFQNRLYQ
                     MFLPYDKGSLASLLHEIGDIKKEEYQADGIFLEAIINQEDLHYFQDYLLT"
     gene            5964..6170
                     /locus_tag="PK015_06020"
     CDS             5964..6170
                     /locus_tag="PK015_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34059.1"
                     /translation="MTNFNIEERLMPESETKITADKHKAYFEEEVISFYRLLVEHIKD
                     SSLISAFEVYDTTDYWNEIMKEVF"
     gene            6241..6903
                     /locus_tag="PK015_06025"
     CDS             6241..6903
                     /locus_tag="PK015_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HOL34060.1"
                     /translation="MFQLISAEEAAAMIKPHSRIALGGFLAVGTPETILDAIVEAGIN
                     NLHIIAITSDYIGKGIGKLVDAHLVKSVQVSHMGTNKNIQDQVNSGEIQSELIPQGTL
                     MERVRAFGAGLGGVLTPTGLGTIVAEGKQIINVDGKDYLLEPAIPSDFALIRAYKADK
                     AGNLIYRKTARNGNPVMAMAGKITIAEVDEILEDEYIDPEAVITPGVFVNYIVLSKEG
                     RK"
     gene            6900..7547
                     /locus_tag="PK015_06030"
     CDS             6900..7547
                     /locus_tag="PK015_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HOL34061.1"
                     /translation="MTNDKRTHIGKRVAQELLDGDYVNLGIGLPTEAANHIPEGRKVV
                     FQSENGIMGVGPAPAEGQEDKDMINAGGGYITAVPGASFFDSATSFAIIRGKHIDVTV
                     LGALQVDQEGNLANWMIPGKLIPGMGGAMDLVTGAKKVIVAMEHCDKHGNPKILKKCN
                     LPLTAPHCVNLIITEMAVIEVTPQGLLLKEITEGYTLEEVLKATEAELIVPDSLK"
     gene            7730..9217
                     /locus_tag="PK015_06035"
     CDS             7730..9217
                     /locus_tag="PK015_06035"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOL34062.1"
                     /translation="MANSSVRLRFASLTVQGKNPAKVNNEDYCGHFQGKYGDLFLLCD
                     GMGGEAGGEIASRMAVESINEYFESHYIEGEESTTIAQSVEYAQYKIIEYTKLNPEMT
                     GMGTTLVLLLIKENSYYFANVGDSRIYLRRGGALLQLTKDHSEVQRMVEQGLISREEA
                     AIHPLRNIITRAIGSSSYRPDISGPHLLQEGDVFLLCSDGLTEYVKDEEILQQLSEEP
                     QIATHNLVDLAIHRGGLDDITVQIVEVLISKPVVIQEEVPASHPNRFYPSLALILTIV
                     VFLVALFLYTQSLKKIPESSKVAPVDTLKEETLKEEETIPPIIEKKEQREEKSVAVFT
                     QLDEKAIDAKLGASAANAHFQEVFNKMSDKKTQAKNLKFFSQTRDNTVIYILPGQTIY
                     LAYGILGTKAGYNMSQEQIEALLAIAIARSSGDSNLNTENWEQELFAPANTPLDEATW
                     KKAQEIYSRYNKANASSLFNSTKRFGKFKTLIRTGKYSIAINKSH"
     gene            complement(9292..11868)
                     /locus_tag="PK015_06040"
     CDS             complement(9292..11868)
                     /locus_tag="PK015_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOL34063.1"
                     /translation="MLKYHQEFNMDEQNYKYLGLSQAEAEARLKKDGYNELPTSKPRN
                     LFKIIWGVLSEPMFILLVSCAVLYLLLGNLEDALVLVGSVILVMAIDIIQERKTDNAL
                     EALKNLSSPRALVIRDGEQKRIPGREVVVGDLIILAEGDRVPADAFILVNEQLNVDES
                     LLTGESVPVRKRVWDGKENAPQPGGDDLPCVYSGTLIVSGRCIAQVYATGAKTELGKI
                     GKSLEGLQEEKTKLSRDTNKLVKNFALLGLVICLIVVIAFYFTRKDLIQAFLSGITLA
                     MSVLPEEFPVILTVFLALGALRLSKKNVLARRAQAIENLGATTVLCTDKTGTITYNKM
                     TVQKIWEINSPHSISLKDIERDNKKDQDLLFFSMLACMKDPFDPMEKAIHKAVKDVDP
                     SLYGNYEALQLVKEYPLTDSCRAMTNVWKEGNSDNLIVAMKGSPETVLDLCKMDSISK
                     ESEMQKVSQMAEEGLRVLGVGMARVDQGNLPEEQSGFDFQYIGLIGLADPIRENVPAA
                     VKECYRAGIRIIMITGDYVGTAIAIGKQIGLKNVENVITGAELDAMDNEELKEKVKKV
                     TIFARVVPAHKLRIVQALKANNEVVAMTGDGVNDAPALKAANIGIAMGQRGTDVAREA
                     SAIVITDDDFSSIVSGVRMGRRIYDNIRKSFTYVFSLHVPISGMSIIPAMFTEFPLIF
                     FPVHIAFLELIIDPACSIVFEMEKEEEDIMDRPPRDPNAPLFGKKMVILGMLQGLAVF
                     IVLALMYFWNLNNGVIPTKNRAMVFTTMVFSNLMLITTNRSWEKNLIKILKTPNPAML
                     YLLLGVFFIVILATNVPAVINIFHLSKLNLVEFIVCLLAGNISVLWFEIYKAVKGKKA
                     LS"
BASE COUNT         3873 a         2104 c         2335 g         3770 t
ORIGIN      
        1 ggaaaaagtg acccgggaac tgtacgaaga gtttcatatg gttctttatc cagagcgaat
       61 tacacttaaa gtcaatgcag atgaactctt taaaatggca gatgatgccg caaaaagtcg
      121 caattttaac gatgctattg ccatttatga ccagataatc cagcattttc ccaatggttc
      181 agatgattat cgtgcctttt ttatgaaggc tttcatcatc gctgaggaac ttaaagatga
      241 agaacgcgct ctccagttgt ttaaggattt cttgaaaaaa tatcctcaag gagacctgaa
      301 tgaatctgct caatttatga ttgatgctct ggaaggcaga attcagttgg aattagaaga
      361 gtaatttaaa aaacttaatt ttatagccct gtttcggcag ggctttttta tttgctctgc
      421 ctataattgt gcagctatat tgattttaaa agaataatat tctaattagg tagacgatat
      481 tatatcatta taataagctt atcttttgac aacaagattg ctatctttat tttatcctat
      541 ctgttacctg cctctcactt agcttcccag tcaaggaaga gacaggaaag agctaagaaa
      601 gagctaaggg agaatgtata tatatttgga atataaaggg ttaagaaagt ggatttggca
      661 aggttaataa caaaatagaa tatattatga caatatttat aactttatta tatctttaat
      721 taattaaatg tatactattt ttatatttta tatttacctt ttaattgcct atttatctat
      781 tgattaggga tttggaattg tcaatctgat tgacaatatt taccatcctg ttgaaaagga
      841 acttattaaa tttttggatt atgatatgga aaaattgaaa actgaccttt ctaatttgga
      901 agaatatttc aattgcaatt ataatgcgga aaaaagtgca tcagctcaga caatttttct
      961 taaaaaatta gctgagctct cgcatcgtta ttatcatgga aagatgcaga ctttgcccaa
     1021 agttggaatc tggaatttta attggttcaa tgtatggtat actcctgggg tttcttctat
     1081 ctctaccact atccgtgata atcctgagga atcctttgaa ttaagcaatc gtgggaactt
     1141 agttgcaatt gtaacagata gcaccagagt tttaggggat ggagattgtg gaatctatgg
     1201 tggtttaggt gtgatggaag gtaaggcaat gttgatgaaa tacctgggag gtttagatgc
     1261 catctctctc tgtgttaatg accgtgatgc gaagggcaac cctgttcctg aaaaacttat
     1321 tgattttgtt aagatgttag aacccagtat tggagctgtt aatctggaag atatttccca
     1381 accaaactgt tattatgttc tggatgaatt acttactggt tgtgaaattc cagtgtggca
     1441 tgacgatgca cagggaactg cctgtgtaac tcttgctggt ttaattaatg cattaaaact
     1501 tgtagataag aagatttttg atataaaagt ggtgctttat ggagcaggtg ctgccaatac
     1561 aaccattgct cgttttttaa tgaaagaagg attagacccg gcaaatctga ttatgtttga
     1621 tagtaagggt gctctacatt ctgatagagt ggatttagca aataatcctg ataaattcag
     1681 acagtgggat tttgcccaaa agaccaatcc caagcattat aatactatgg aagaagcaat
     1741 gaaaaaagct gatgttctaa tctctctttc cactccggga cctgatataa taaaaccaga
     1801 atggattaaa ttaatggcag aaaaacccat cgttttcgcc tgtgccaatc ctgttcctga
     1861 aatttatcct tatgctgcaa aatcagctgg agcttttatt gttgccacgg gaagaggaga
     1921 ttttcctaat cagataaata attccttagg ttttcctggt atcttaaaag gagctttaat
     1981 ggttcgttct cggcacataa cagatacaat ggctattaaa gctgcttatg ctcttgccac
     2041 ttttgcagag caaagaggaa tatcaccaga taatattatc cctcaaatgg atgaagtaga
     2101 tgtctttcct tatgaagcag cagaagtcgc aagccaggca atgcaagaag gagttgccag
     2161 gaaaactaat agttatgaag gtttatatca acaagccaga acagatatat tacaagctcg
     2221 ggagacctgt cagaaaatga taaaagatgg aagtatccct cctattccag aaaaattgat
     2281 tgaggaagca ctacaacata caaataagat gatttctaaa taagatgcaa aaacagaaga
     2341 agaaaaacaa cctgcggggg gtcctttctt ttattctaaa gctgggtatt actatcctga
     2401 ttatgtggag tatattcagt aaattgaatt taaaagtagt aattagggaa tttgtcactc
     2461 aaccagtttg ggttatactt gttcttatag gaattactgt tctgagacac tggggacaat
     2521 atctaacctg gaaatgggca cttcaagtca atcccaatta tcatccagct aaatatgaga
     2581 ttttcaattc ttatatgatt ggacaggcac ttcgctttgc cattccagga agttttggta
     2641 tgataggtaa aatagcgttt gtacataacg atagtaagat taattcttta tattcgttta
     2701 ttttggagcg aatttttgta acttgggttt tgctattttt tgccagtatg gctctggtat
     2761 tcgtagattt aggtattcct tcctggattt cctggctctt atttttaata tttgatacta
     2821 tgcctttctg gatgtatttc ttactttctg tagaccgacg tttgaaaaaa cttcaatcag
     2881 gatatctgaa attagctcct gcaatggtct ttgcccagat tgttattact ctcctaaatt
     2941 atatacaata ctggatttta ttggaacaag tgcaacctgt cagtttctgg gaaattatga
     3001 atagaatgtc tctaagccat cttgcctata gcattcccat cacttttgcg ggattaggag
     3061 tgaaagaaaa attggctata cctctgcttt ctgatattgg ttatctttca gaaaatattg
     3121 taagtactac tcttatcatc tttatcattc acgatgtggc tgctgcttta attggagcag
     3181 tgatattttt aagagttaaa agatctaaat agatattatt atatgcctga gattaaaata
     3241 attggagcag gtttagcagg aagtgaagct gccttatatc ttgcagataa gggttggaaa
     3301 gttaagcttt atgagatgag acctgcgaaa atgacccctg ctcatcaaac tggatatcca
     3361 gcagaactgg tttgtagtaa ttctctaaaa tccacccttt tagacacacc tgctgggttg
     3421 cttaaagagg aacttcatct gctcggttca aaacttttac ctgtagctga aagctgtgca
     3481 gtctctgctg gacattctct ggcggtagat cgtaatttgt ttgcccaaaa gattaacgat
     3541 gaaataattc accatcctaa tattgaattt attcgtgagg agataaccac tcttccaaca
     3601 gagttaagta ttctggcaac aggaccctta acttctgata atcttattca ggagctccaa
     3661 tccgtggtcg gttcggctca tctttatttc tatgatgcaa ttgctcctat tgtctcagct
     3721 gatagtctaa atatggataa aatttacttg aaagatagat acgataaagg tgagccagat
     3781 tatctaaatt gtccatttac tgaagaggaa tactaccatt ttgtggaggc tttattaact
     3841 ggtgaaaaac atcccccgca ttcctttgaa aatgaaatga cggaggctaa gctaaagttc
     3901 tatgagaatt gtatacctat tgaagagctt gcccgaagag ggaaagatac cttgagattc
     3961 ggggttatga gacctatagg actggaaaaa gaaggtaaaa ctccttttgc tgttctgcaa
     4021 ttgcgtcctg aaaataagga acataccgct tataatttag tcggttgtca gactatgctt
     4081 cgttattctg agcaaaaaag aatattccga ctcattccag gcttagaaaa agctgaattt
     4141 ctacgctatg gttcactgca tcgtaatagc taccttaatg ctcccgcagt actaaatccc
     4201 gagctcaatc ttaagaatgc acctcatata tatgtggctg gacaactaag cggtgtggaa
     4261 ggttatgtgg aatgcattgc ttcaggtctc ttggttgcta agattatttc agaaggttta
     4321 gctatgcttc ctccagaaac gattactggt caaatctggc gtcgtttaat tgatacctct
     4381 atcaagaatt ttcaacccgt aaatgctaat tatggtctgc ttcctgctct aaataatccc
     4441 ctgcgagaca aaaaacaaaa gaagttagct ctttcagaaa gaagtattaa agctttgaaa
     4501 gatttcttgg ataataagtg attgcgttat tgacataaaa atatggtttg agctaatgac
     4561 tttcaggagt taaaaatatg ctggatgaag agaaagatta tctggtagaa gaattttata
     4621 atgaaccaga tacctgggaa aaaggagagg aagaggaaaa gactgctttt ctggctactc
     4681 tggctacccg tggtgaaaag gaatcagaaa ctgaagccag cctaaatgaa ctgggaaaac
     4741 tggctgaaac tgctggtatc gctgtgttgg gcaaatatac tcaaaagcgt gaaaatcctg
     4801 agagtagcac ttattttgga aaaggttttc tggaagaatt gagtagaaag atgcatcaga
     4861 ataatgccga tttcttgatt gttaatgatg aattaactcc agttcaggca agaaacattg
     4921 aaaatgatta tggtattaaa gtgatggata ggacagaaat cattctttct atttttcatc
     4981 aacatgctca taccaaagaa gcaaaattgc aggttcgttt agcggaatta caatatcagc
     5041 ttcctcgttt gagaagacaa tctgaaaatt atgaccagga acatggtgct gtccgttctc
     5101 gtggtggttc agcttcccgt ggaatggggg aaaaacagat agaagttgat aaaagaatca
     5161 ttaaagataa tatccgcaaa atcaaacagg ctattgcgga aatatccaag aataaagaaa
     5221 ctcaacggaa acaacgagaa aagagtaaaa aaatctgtct ggtaggttat actaatgcag
     5281 ggaaatcaac cctttttaat gccttgacgg aagcaggagt tctggtagaa gacaagctct
     5341 ttgccacgct ggattctacc acccgtcaat taaagctttc cacttccttt cctgtcgtta
     5401 tctctgatac agttgggttc atctctcgtc ttccacatca attagtcgct tcttttactg
     5461 ccactttgat ggaagttgaa gatgcagacc ttttacttca catagtagac ctttccgatg
     5521 aacgctatga atattacatt gaacaggtgc aatcagtgct aaaagaaatt ggggctgata
     5581 acattcctca gatactggtt tttaataaga tagatttagt tgattctatc caattaagtc
     5641 ttgctaaaaa gcgatatccc gatgccatat ttatttcagc cttgaataaa agccaccttg
     5701 aagaccttct gcaaaaagtg gaagagaaac tttttcagaa cagactctat cagatgtttt
     5761 tgccttatga taagggttcg ctggcatcgc ttttacacga gattggtgat atcaaaaaag
     5821 aagaatatca agcagatggt atctttctgg aagcaattat aaatcaagaa gatttacatt
     5881 actttcagga ctatctgttg acataaaact ctgttgttaa taagcagtat ttcagttact
     5941 gcttatataa aaataatgat tttatgacta actttaatat agaagaaaga ttgatgccag
     6001 agagtgaaac aaaaattact gctgataaac ataaagctta ctttgaagaa gaagttataa
     6061 gcttttatag attactggtt gaacatataa aagatagctc tttaatatct gcatttgaag
     6121 tgtatgatac cacggactac tggaatgaaa ttatgaaaga ggtattttaa acgaggctca
     6181 ggaaaagtaa gtggaaaaaa tgaaggtttt tgataaaaga taaatataaa ggagacaatt
     6241 atgtttcaat tgattagcgc agaagaagca gctgcgatga ttaaacctca cagtcgtatt
     6301 gcattaggtg gatttttagc ggttgggact cctgaaacca tccttgatgc tattgttgaa
     6361 gctggtatca ataatctgca tattattgct atcacttccg attatattgg caaaggtatt
     6421 ggcaaactgg tggatgcgca tctggtgaaa agcgtccaag tttctcatat gggcacaaac
     6481 aaaaatattc aggaccaggt taattccgga gaaattcagt ccgaacttat tcctcagggc
     6541 actttaatgg aaagggttcg tgcttttggt gcaggtctcg gaggtgttct cactcctaca
     6601 ggtttaggca ccatagtagc tgaaggaaaa caaataatta atgtggatgg taaagattat
     6661 ttattagagc cagctatacc aagcgatttt gccctgataa gagcgtataa agctgataaa
     6721 gcaggtaatc ttatatatcg taaaaccgct cgtaatggca atcctgttat ggctatggca
     6781 ggaaaaatca ctattgctga agtggatgag attttagaag atgaatatat agaccctgaa
     6841 gcagtgataa ctcctggggt atttgtcaat tatatagttt taagcaaaga ggggagaaaa
     6901 tgactaacga taaacgtact catattggaa aacgtgtagc acaagaactg ctggatggag
     6961 actatgtcaa tctgggcata ggtttaccca ctgaagcagc caatcatatt ccagaaggaa
     7021 gaaaggtagt ctttcaatca gaaaatggca ttatgggagt tggtcctgct ccagcagaag
     7081 gacaagaaga taaggatatg attaatgctg gtggtggtta tattactgct gttccaggtg
     7141 cttctttctt tgattctgcc accagttttg ccataattcg cggtaagcat attgatgtta
     7201 ctgtacttgg tgcattgcaa gtagaccagg aaggaaatct ggcgaactgg atgattcctg
     7261 gaaaacttat ccctggtatg ggtggggcaa tggacctcgt gacaggagct aaaaaagtca
     7321 ttgttgctat ggaacattgt gataagcacg gcaatccaaa aattttaaaa aagtgtaacc
     7381 ttcctctaac tgctcctcat tgtgtgaatc ttatcattac tgaaatggct gttatagagg
     7441 tcactcctca aggtttattg ctcaaagaaa ttactgaagg atataccctg gaggaagtac
     7501 ttaaagcaac agaagctgaa ttaattgtcc ctgatagtct caaatagaat atctcataga
     7561 cactgtaaag aaagtaaaaa ctggaagtgt aataattgtt aactcactgt aatctttcta
     7621 tacacctgat ttaataataa gaaattcttt ggagtcagag tcttttagga gtttaaagta
     7681 tattatttgg attaaataga taggaatcta aagagcagga gtgttaatta tggctaatag
     7741 ctcggttcgt ttaagatttg ccagtttaac ggttcagggt aaaaatcccg ctaaggttaa
     7801 taatgaggat tattgcggtc attttcaagg caagtatggt gaccttttct tactctgtga
     7861 tggaatgggt ggagaagcag gtggagaaat tgcttctcgg atggcagtag aaagtattaa
     7921 tgaatatttt gaatctcatt atattgaagg agaagaaagt actactattg ctcaaagtgt
     7981 agaatatgct caatataaga taattgaata caccaagcta aatccagaaa tgacgggaat
     8041 gggtaccact cttgtacttt tgctgattaa ggaaaattct tattattttg ctaatgttgg
     8101 agatagtcgt atttatcttc gtcgtggcgg tgctttatta caacttacca aagaccattc
     8161 tgaagttcaa agaatggttg aacaaggctt aatttctcgt gaagaagctg ctattcatcc
     8221 actcagaaat attatcactc gtgccatagg ttcatcgagt tatagaccag atatatcggg
     8281 acctcatctc ttgcaagaag gagatgtctt tttgctctgc tctgatggct tgacggaata
     8341 tgttaaagat gaagaaatct tgcagcagtt atctgaagag ccacaaattg ccactcataa
     8401 tctggttgat ctcgctattc atcgtggtgg tttagatgat atcactgttc agattgtaga
     8461 agttcttatt agcaagcccg tagtaattca agaagaagtt cctgcttccc atcctaatcg
     8521 gttttatcct tctcttgctc tcatcttgac tattgttgta tttttagtag ctttattctt
     8581 gtacactcaa tccttgaaaa agatacctga gtcctcaaaa gtagctccag tagatacttt
     8641 gaaagaggag acactaaaag aagaagaaac catacctcct attatagaaa agaaggaaca
     8701 aagagaagag aaatccgtag ctgttttcac tcaattagat gagaaagcta ttgatgcaaa
     8761 gcttggtgca agtgctgcaa atgctcattt tcaggaagtt tttaataaga tgagcgataa
     8821 aaagacccag gcaaagaacc taaaattctt ctctcaaacc agagataata cagtcatcta
     8881 tatcctgcct ggtcaaacca tttatcttgc atatggaatt ctgggcacga aagcaggata
     8941 taatatgagt caggaacaaa tagaagctct tcttgctatc gctattgcca ggtcttccgg
     9001 tgattctaat cttaatacgg aaaactggga acaggaactc tttgctccag cgaacactcc
     9061 tttagatgaa gctacctgga aaaaagcaca ggaaatctat agcagatata ataaagctaa
     9121 tgcctcatct ttatttaata gcaccaagcg gtttggtaaa ttcaagactc ttataagaac
     9181 aggtaaatat agcatagcta taaataagtc tcactgataa agcatttgag agagagataa
     9241 agggtttttt agatagtaat ccctttatta taaatagcta aatggtttag cttaactgag
     9301 tgcttttttg ccctttactg ctttatagat ttcaaaccag agaacgctaa tgtttcctgc
     9361 taacaggcaa acaataaatt ccactaaatt cagtttagaa aggtgaaaga tatttataac
     9421 agcagggaca tttgtagcta aaatcacaat gaaaaagaca ccaaggagaa gatataacat
     9481 tgctggatta ggtgttttga ggatttttat caggttcttt tcccaggaac gattagtggt
     9541 tatgagcata agattggaaa ataccatcgt ggtaaagacc atagcccgat ttttagttgg
     9601 aatgacccca ttatttaaat tccagaaata cattaatgct aaaacaatga ataccgctaa
     9661 tccctgaagc atacctaaga taaccatctt tttcccaaaa agaggggcat taggatcacg
     9721 aggaggacga tccattatat cttcttcttc cttttccatt tcaaagacta tggagcaagc
     9781 agggtcaatt atcaattcta aaaaagctat atgaacgggg aaaaaaataa gggggaattc
     9841 cgtaaacatt gctggtatga tagacattcc agagataggt acatgtagag aaaagacata
     9901 agtgaaggat ttacgtatat tatcatatat gcgtcttccc attcttacac cactcacgat
     9961 ggaagaaaaa tcatcatcag taattacaat ggcagaagct tcacgagcta catctgttcc
    10021 tctttgtccc attgctattc ctatatttgc cgctttaaga gcaggtgcat cattaactcc
    10081 atctccggtc attgcaacta cctcgttatt agcttttaat gcctgaacta ttcttaattt
    10141 atgtgctggt acaactctcg caaaaatggt taccttcttt actttctctt ttagctcctc
    10201 attatccata gcatccaatt ctgcaccagt tatcacattt tctacatttt tcagaccaat
    10261 ctgtttccct attgctatag ctgttccaac ataatctcca gttatcataa taattctaat
    10321 tcctgctcta taacattcct taactgcggc aggaacattt tcccgaattg gatctgccag
    10381 accaatgagt ccaatgtatt gaaaatcaaa gccagactgt tcttcaggta aattaccttg
    10441 atcaactctt gccattccaa ctcctaaaac gcgcaatcct tcttcagcca tttgagagac
    10501 tttctgcatt tcactttctt tggaaataga atccatttta cataaatcca gaactgtttc
    10561 aggagagccc ttcatagcaa ctataagatt atctgaattt ccttctttcc atacatttgt
    10621 catagctcta caactatctg taagtggata ttccttcacc aattgcaatg cttcataatt
    10681 tccataaagg gagggatcaa catctttaac tgctttgtga atagcttttt ccattggatc
    10741 aaaaggatcc ttcatacagg caagcatact gaaaaatagc aagtcttggt cctttttatt
    10801 gtctcgttca atatctttaa gagaaataga atgaggactg tttatttccc aaatcttttg
    10861 cacggtcatt ttattatagg taatagtgcc agtcttgtct gtacatagaa cagttgttgc
    10921 accaagattt tctatagctt gagctcttcg tgctaataca ttctttttag ataaacgcaa
    10981 ggctcctaaa gcaaggaaga ccgtaagtat cacaggaaat tcttcgggta aaacggacat
    11041 tgctaaagtt attccactga ggaaggcttg aatcaaatct ttacgagtaa aatagaaggc
    11101 aattaccact atgagacata taaccaagcc taaaagagca aagttcttta ccagtttatt
    11161 agtatcacga ctgagtttgg tcttctcctc ttgcaaacct tctaatgatt tacctatttt
    11221 acctaattct gtttttgctc ctgtagcata aacctgagcg atacatctgc cacttacgat
    11281 caaagtgcct gaataaacac atggtaaatc atctcctcca ggttgaggtg cattttcttt
    11341 accatcccac actcttttac gaactggtac cgattcccca gttaacaaag attcatctac
    11401 atttaattgt tcatttacta aaataaaggc atctgctgga actctatctc cttcagctaa
    11461 tattatcaaa tctcctacca ccacttctct tccaggaata cgtttctgtt ctccatctcg
    11521 tataaccaaa gctcgtggac tggataaatt cttaagcgcc tctaaagcat tatctgtttt
    11581 cctctcttga ataatatcaa ttgccattac caggataact gaaccaacca gaactaaagc
    11641 atcttctaaa ttacctaata atagatacaa tacagcgcaa gaaacaagaa gaataaacat
    11701 aggttcactt aaaacacccc atattatttt gaacagattg cggggtttgg aggtgggtaa
    11761 ttcattgtac ccatcttttt tcaatcttgc ttctgcttca gcttgcgaaa gtccaaggta
    11821 tttataattc tgttcatcca tattaaactc ctgatgatac tttaacatcc aaaatccttt
    11881 gtccgtcaag aaaatttcag ttgatgctaa atgagaaact aaaattataa gcccaaattt
    11941 tatgtgaaat tggagaaata ggcaagccac taaggaagga attgtttttt tgaaatctgg
    12001 gagtgttcaa ataaatgggt ctaaaaagag actcaaagta tcttgataaa aaggttaagg
    12061 ctaatttata ctcttgcgat ga
//
LOCUS       DAORZF010000048        11094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_4488_length_11094_cov_360.238337, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000048 DAORZF010000000
VERSION     DAORZF010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 11094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11094
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4488_length_11094_cov_360.238337"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..1625
                     /locus_tag="PK015_06045"
     CDS             147..1625
                     /locus_tag="PK015_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34064.1"
                     /translation="MKSLKYRIKLTAQTIISGTSGDSVTNSCLSYIPGTTILGILAGR
                     YLQQYNDKTEFERLFLRRGLFFRNSYLQKDGLNYIPCPKNILQNKKDESDLLVLNEET
                     IKNTENLLDYKEVNSFVNIEDDNIKLQNPEFCLNFHHQRNYTTGIPEENVVFNYEALK
                     PDQIFAGEIIGEEKDLQLISKFLKEKEVRIGKSKTAQYGNAEIIDYLIDDLPPLENFS
                     PPIYLYCVSDLILLNEFGYPITSVKGLEKVLGVTVLEAYTESGRTESTVVAWKAKKPS
                     YHTLKAGSIFLLDKLPDNYQDILIYGLGERTWEGFGQVEFMELPEKVSIRREYKKAKI
                     PKPKTDIPFILEDVIERIYKNLIYRELKHKAMEKANTLEYTPLSKSLVAKLEGFSLQD
                     DFFVMLTQLNKTSVDKLRRTYIGDMNLYEFLAEDNINTLVTNIISSKEILGNSDSKLK
                     DLRDEFMIEQISIKEARKKYLVNFFWFLRKKLAGETKKEENK"
     gene            1622..3031
                     /locus_tag="PK015_06050"
     CDS             1622..3031
                     /locus_tag="PK015_06050"
                     /inference="COORDINATES: protein motif:HMM:NF015728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAMP superfamily CRISPR-associated protein"
                     /protein_id="HOL34065.1"
                     /translation="MNYYGKIILKADLELISPLMIGSGESEISDRDILLNKEDKPYIP
                     ASSFMGKLYNELSSDKKKKYFGQKKVEENDKNEEENKAESNKFEHQSYIICDDLHLSD
                     DSVKNTAIRDGIRINPETGLVEKGAKFDYQVLEPGNHFALEMSINVEPCTYGDCKELM
                     KNIVNVLSNRQFALGGKTSSGFGNLQAQNIKVMEYDFADKKQAAAYLLKKETENLFSE
                     YIEENPMLNDEFRINASFQIVNSFLIRSYSKTPYGSDATQIKCGDKFVLPGTSLRGAL
                     RARAQKILNLLWENKKDEVDMFIAALFGTTSLEKKNEYSVPSSLYVSETVIDNVECQL
                     QNRIQIDRFTGGTIESALFDSMPVFPVPNDEAQLVNLTLTIHKPLPSQKGLLLLLLKD
                     LYTSELPIGGEKNVGRGLLKGTKATVTNGDQSIHFSNFEDIDEATQKLFNQYIEALIS
                     KSDNEAIEEYIAKFKKAKA"
     gene            3040..3612
                     /gene="csx19"
                     /locus_tag="PK015_06055"
     CDS             3040..3612
                     /gene="csx19"
                     /locus_tag="PK015_06055"
                     /inference="COORDINATES: protein motif:HMM:TIGR03984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Csx19"
                     /protein_id="HOL34066.1"
                     /translation="MMEELPKIQELKCRIEYINKLESLEDINQYLEDKDYLVYIITLS
                     GISFGEFSNYQFKLIEDITKLDRLEELRIFDKERELHIWRQSDGKGGSVLKGRLRIDN
                     PIEEAEQCYYRDAKQLLFGTKRESLQNGFTFLKEDRGFGVIIPNSLLSKLKDNPRLYL
                     CTRNYLEEWDNGQLSYCDSRFISIEGVNND"
     gene            3605..5734
                     /locus_tag="PK015_06060"
     CDS             3605..5734
                     /locus_tag="PK015_06060"
                     /inference="COORDINATES: protein motif:HMM:TIGR03986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03986 family CRISPR-associated RAMP protein"
                     /protein_id="HOL34067.1"
                     /translation="MTKATLYVSRSKKGAVVLDIQPENAGKRSLPDSVFREDEKIKSL
                     EPGEYEVEIELEENTKKIVALSIDDHTIRLPEFKPVPKYQPKQEKSQLKETTTSPEQA
                     YAPYNFVSLDDIVIFYNKDALPRRNSFTGLSGRINLNIETKTPLYIQRTLTEKEVEEL
                     TKIEKEENNKEKIRDFNRKIYQSFSPASGKYKIPGSSLRGMVRSLYEIATYSKMSFLD
                     DRKLYFRAFADRAKLLQEYYNKRMINEDKNAIPRLKVKAGYLFKSGKDFYINEAENYY
                     RVEEDKVLNEDFIKLPFSKKGNDEKSKYDDYITANPYLKIGYSLKNHIVRNGFIIINN
                     IMPYNEVLPEDYQEGYIVFSKGIPLSSKTKRKYWVVGKKTDKKHKIDETVVNDYYNDK
                     NRNSINLIDYLNRNKKEYTNGIPCFFLYEDGQVTAFGNTALFRISYKNSIGKLLPENH
                     KSDELDMAEALFGRVMKKQEDTINGRIYFDDAVAKEAVAFEKPIYPKILANPKPTSFQ
                     LYLKQPSDVTFDTIRHYDSPDAKLRGYKQYWHKKGDKFEEDSPDFDPQKDTQHTLIAP
                     IKPGAIFEGSIRFENLSEEELGALLFVLNLPEGYCHKIGMGKPLGLGSIKITATLSIL
                     DPKKRYSNLTDNGYTNDDPSKYLDAYKELLKKHHILNTENPWDLMRFRELKKMLDFAN
                     HPDESKTEYMPLDQFRERGVLPEPLKV"
     gene            complement(5768..7081)
                     /locus_tag="PK015_06065"
     CDS             complement(5768..7081)
                     /locus_tag="PK015_06065"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34068.1"
                     /translation="MIELEEIRKKIYGAIKGTELEYCCYFAGGCVRDMYLNRERTDLD
                     ICVEKEEGDKLLANILQEKGLIRKVVRYQRTDTLMTKIDGYSLELSPTQIRTEMDNGK
                     VHKTFASVEEDARSRDFTINALMIAIHSGDLLDFTQKGIKDLESRLIRACESEGKGFV
                     KDPIRILRAVRLAAELDNFTIEEKTGAEIQKKSFLLEELNPLRITRELEKILLLKSFF
                     KAQYLLQSFQLMSHLLSEFNNITLDEKTLNWLKHPELSKLIQTLESLEGRFCFLCLPF
                     YLNRTDSNSEFKVNLEPLISYHRYFSNKQNRHIDNLLYAIRVFLNEFYLKSEMELSHL
                     REAIHKVNDDFEELGILLQCVDNYKGWRSGTKSALSKLAIELKNEMDANPFHITGTEF
                     MEQYQIKEGKEIGVYLQRALTIWYQNPKLSMPEIMDLAINLPIEK"
     gene            complement(7194..7748)
                     /gene="frr"
                     /locus_tag="PK015_06070"
     CDS             complement(7194..7748)
                     /gene="frr"
                     /locus_tag="PK015_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOL34069.1"
                     /translation="MDEILDECKEKMKKTFDAMLHQFSKIRTGRASASVLDDIRINYY
                     GQSTPIKQLCNISIPEPRMIIVQPWDKTTLADIEKAILAANLGVTPENDGNVIRLPFQ
                     PLTEEKRKDIAKNLKKIAEDTRVAIRNIRREANEQVKKLEKDGEISEDEQKKMLKDIQ
                     DITDEWIKKIDEAEKSKEKEILEV"
     gene            complement(7776..8498)
                     /gene="pyrH"
                     /locus_tag="PK015_06075"
     CDS             complement(7776..8498)
                     /gene="pyrH"
                     /locus_tag="PK015_06075"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOL34070.1"
                     /translation="MLFQPEKIHRVMLKISGEILAGPQGYGFDDEVIDRLTDEIIKVR
                     QKGYSIAIVLGGGNIFRGGTWKNQNLDRVVLDHIGMLATIQNSLYLAEILNLKNCPAR
                     AFSCLIMDNITELYTPSKASEALETGQICFIGGGTGNPFFTTDTAAILRAIELQSDLV
                     LKGTKVDGLYTSDPKVDSNAQFISSASFSQCLEQKLGVMDMTAFSLALENRIPLKIFN
                     LTRAGGIEMALTQPDFGTYIYA"
     gene            complement(8537..9136)
                     /gene="tsf"
                     /locus_tag="PK015_06080"
     CDS             complement(8537..9136)
                     /gene="tsf"
                     /locus_tag="PK015_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOL34071.1"
                     /translation="MQEITAKQVKELRDRTGVGILECRKALEATNGDMEAAIKYLRER
                     GIAKAESKASRQTKEGLIHAYIHFNGRLGVMLELNCESDFVARTEQFKKLAQELALQI
                     AATNPLAITSEQIDPAIIEREREIARNKAINEGKKPEIVEKIVEGTLKKFCSENALLE
                     QELVNEPGVTVRDLINSTIAQTGENIQISRFVRYALGEE"
     gene            complement(9200..10045)
                     /gene="rpsB"
                     /locus_tag="PK015_06085"
     CDS             complement(9200..10045)
                     /gene="rpsB"
                     /locus_tag="PK015_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOL34072.1"
                     /translation="MSVVTMKQLLEAGVHFGHQTYKWNPKMKKYIFLKRNGIHIIDLK
                     QTVDAINEAYQFLREVASRQEYILFVGTKKQAQAAIKEAAEKAGVFYVNQRWYGGMLT
                     NMQTIRQSIEKMKYYESIVADGTINKYTKLEQLKMKRMHDKIELYLGGIRDMDALPGA
                     MFVVDVGHEAIAVHEARILHIPIIAMVDTNCDPDLVDYVIPANDDATRAIQLISDIMA
                     NAVLEGKNIATEGEGATLEMPVIGDEFTPEMIEMATTPPKDEEEEEEIVTTEELTETI
                     AQPEA"
     gene            complement(10196..10582)
                     /gene="rpsI"
                     /locus_tag="PK015_06090"
     CDS             complement(10196..10582)
                     /gene="rpsI"
                     /locus_tag="PK015_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOL34073.1"
                     /translation="MQTYNAVGRRKNAVARVRLMPGTGKRIINEVQMKKYLQRETLAM
                     IIEQPLQELGLSDNFDVYVNVSGGGLSGQAGAIRHGIARALVEFDENLRPQLKARGYL
                     TRDPRMVERKKSGRPKARKRFQFSKR"
     gene            complement(10595..11023)
                     /gene="rplM"
                     /locus_tag="PK015_06095"
     CDS             complement(10595..11023)
                     /gene="rplM"
                     /locus_tag="PK015_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOL34074.1"
                     /translation="MKTLTPKPGDIERTWYVIDATDQPLGRLSSKIASILCGKHKPYY
                     ARNIDTGDYVIVINAEKVKVTGLKSIQKVYHSFSGYPSGLKDIPYSRMLEKNPTKIIE
                     HSVRGMMPKTVLGDAMFKKLKVYAGNEHPHSAQKPVELKL"
BASE COUNT         3621 a         1969 c         1943 g         3561 t
ORIGIN      
        1 gagaaactat tatcttttat ttccttatat ctggatagaa tgggcagtaa acgcaaccgt
       61 ggttttggca aaattgaggt agaaatcttt gatgaaaagg gtaactcttt aactaagaag
      121 ctaagagaaa aactggagaa agaactatga aaagcctaaa atatagaatc aaactcactg
      181 cacaaactat tatctcagga acttcagggg attcagtgac taattcctgc ctatcctaca
      241 ttccaggaac cactattttg ggaatacttg caggaagata tctgcaacaa tataacgata
      301 agacagaatt tgaaaggctg tttcttcgta ggggtctttt tttcagaaat tcgtacttac
      361 aaaaagatgg gttaaattat attccttgtc ctaagaatat cttgcaaaac aaaaaagatg
      421 aaagtgattt attggtactt aatgaagaaa ctattaaaaa tacagaaaac ttgctggatt
      481 ataaagaagt taattccttt gtaaatatag aggatgataa tattaaactt caaaacccag
      541 aattctgctt aaactttcat catcaaagga attataccac gggcattcct gaggaaaatg
      601 tagttttcaa ttatgaagca ttaaaaccag accagatatt tgccggagaa attattggtg
      661 aagaaaaaga cttgcagtta ataagtaaat ttcttaaaga aaaagaggtt agaattggta
      721 aatccaaaac tgctcaatat ggtaatgcag aaatcattga ttacctgatt gatgatcttc
      781 ctcccttaga aaatttttct ccacctatat atctttattg tgtctcagat ttaattcttc
      841 taaatgaatt tggctatcct attacatccg taaaaggttt agagaaagtc cttggggtaa
      901 cagtattgga agcctacacc gaaagtggaa gaactgaatc tacggtagtt gcctggaaag
      961 ctaagaaacc ctcatatcat accctaaaag caggttccat ctttttatta gacaaacttc
     1021 ctgataatta tcaagatatc ctaatctatg gtttaggaga aagaacctgg gaaggttttg
     1081 gtcaggtaga atttatggaa ctgccagaaa aagtttctat acggcgtgaa tataaaaagg
     1141 ctaaaatccc aaaacctaaa accgatattc cttttatttt agaagatgta attgaaagaa
     1201 tatataaaaa tcttatatat agggaattga aacataaagc gatggaaaag gcgaatactt
     1261 tagaatatac ccccctaagt aaaagccttg tagctaaatt ggaggggttt tcattacagg
     1321 atgatttttt tgttatgtta acccaattaa ataaaacctc agttgataaa ttgagaagaa
     1381 cttatatagg cgatatgaat ttatatgaat ttctggcaga agataatata aatacattag
     1441 tcaccaatat catatcttct aaggaaatcc ttgggaattc tgattcaaag ctaaaagatt
     1501 tacgagatga atttatgatt gaacaaatta gtataaaaga agcacgtaaa aaatatctcg
     1561 ttaatttctt ctggttcttg cgcaagaaac ttgccggtga aacaaagaag gaggaaaaca
     1621 aatgaactat tatggaaaga taattttaaa agctgactta gaattaatca gtcctctaat
     1681 gataggtagt ggtgaatctg aaatcagtga tcgtgatatc cttttaaata aagaggataa
     1741 accttatatt cctgccagtt cctttatggg gaaattgtat aatgagctct cttctgataa
     1801 gaagaaaaag tattttgggc aaaagaaagt ggaggaaaat gataaaaatg aggaagaaaa
     1861 taaagcggaa tcaaataagt ttgagcatca atcctatatc atttgtgatg acttacactt
     1921 aagtgatgat tcagttaaaa acactgccat aagagatgga ataaggatta atcctgaaac
     1981 agggttagta gaaaaaggag ccaagtttga ttatcaggtt ctggagcccg gaaatcattt
     2041 tgcactggaa atgagtatta atgtagaacc atgtacttat ggtgattgta aggaattaat
     2101 gaagaatata gtaaatgtgc ttagtaatag gcaatttgct ttaggtggaa agaccagttc
     2161 tggatttggt aatcttcaag ctcaaaatat caaagtaatg gaatatgatt ttgcagacaa
     2221 aaagcaagct gctgcttatc tcttgaaaaa agagacggag aacctatttt ctgaatatat
     2281 agaagaaaat cctatgctga atgacgagtt tcgcattaat gcctcttttc agatagtaaa
     2341 ttcttttctc attcgttcct attccaagac tccttatggc tctgatgcca ctcaaataaa
     2401 atgtggagac aaatttgtgc ttcctggaac ttctttacga ggagctttaa gggcaagagc
     2461 acaaaagata ttaaatctgc tatgggaaaa caaaaaagat gaagttgata tgtttatagc
     2521 tgctttgttt ggcactactt ctctggaaaa gaagaatgaa tactctgttc cttcttcact
     2581 ttatgtttca gaaacagtta tagataatgt tgaatgccaa ttgcaaaaca gaatccagat
     2641 agatcgtttt actggaggaa ctatagaaag tgctcttttt gattctatgc ctgtattccc
     2701 agttccaaat gatgaagccc aactggttaa tctcacattg accattcaca agccccttcc
     2761 ttctcaaaag ggattattac ttcttctctt aaaagactta tatacctctg aacttcccat
     2821 tggaggtgaa aaaaatgtag gcagaggatt attaaaagga actaaagcaa cagtaactaa
     2881 tggtgaccaa tcaatccatt ttagtaattt tgaagatata gatgaagcaa cacaaaagct
     2941 ttttaatcaa tatatagaag cacttatatc aaaaagtgat aatgaagcaa tagaagaata
     3001 tatagctaag tttaagaaag caaaggctta ggagtaaaaa tgatggaaga attacccaag
     3061 attcaagaac taaaatgtag aattgagtat attaataaac ttgaaagtct tgaagatata
     3121 aatcaatatc tggaagataa agattacctg gtttatataa taactctttc aggcatcagt
     3181 tttggtgaat ttagtaatta tcagtttaaa ctgattgaag atataacaaa actggataga
     3241 ttagaagaat taagaatatt tgataaagaa agggaattgc atatctggag acaatcagat
     3301 ggtaaaggtg gttcagtttt gaagggtaga ttgagaatag ataatcccat tgaagaagct
     3361 gaacaatgtt attatcgtga tgccaaacag cttctttttg ggacaaagcg agagtcctta
     3421 caaaatggtt ttacttttct taaagaagac agaggttttg gcgtgattat tcccaattct
     3481 cttttatcaa aattaaaaga taatccgaga ttatatcttt gtactcgtaa ctatctggaa
     3541 gaatgggata atggacaatt gagctactgt gatagccgtt ttatatccat tgagggggta
     3601 aataatgact aaggcaacat tgtatgtaag tcgttctaaa aaaggagcag tagttttaga
     3661 tattcaacca gaaaatgcgg ggaagagatc tctacctgat tctgtattcc gagaagatga
     3721 aaaaataaaa agtcttgagc ccggagaata tgaagtagaa attgaattgg aagagaatac
     3781 taaaaagatt gtagcattaa gcatagatga ccatactatt aggttacctg aatttaagcc
     3841 agttccaaag tatcaaccta aacaagaaaa gtcacagtta aaagaaacca caaccagtcc
     3901 ggaacaagct tatgctccct acaattttgt ttctctggat gatatcgtaa ttttctataa
     3961 taaagatgct ttacctcgta gaaactcttt tactggttta tcaggaagga ttaatcttaa
     4021 tattgagacc aagacccccc tttatattca gagaacatta acggaaaaag aagtggaaga
     4081 actaaccaaa atagaaaagg aagagaataa taaagaaaag atacgggatt tcaatagaaa
     4141 gatttaccag tccttcagtc cagcttcagg gaaatataaa ataccaggtt cttccttaag
     4201 agggatggtt cgtagtttat atgaaatagc cacttacagc aaaatgagct ttctggatga
     4261 ccgtaaactt tactttcgtg cttttgctga tagagctaag ttattacaag aatattataa
     4321 taaaagaatg attaatgaag ataaaaatgc tatacctcgg ttaaaagtaa aagcaggata
     4381 tctctttaaa tcaggaaagg acttttatat taatgaggca gaaaattatt atagagtaga
     4441 agaagataag gtactaaatg aagattttat aaaacttcct ttttctaaaa aaggtaatga
     4501 tgaaaaatca aaatatgatg attatatcac tgctaatcct tatcttaaaa ttggctatag
     4561 tttgaaaaat catattgtaa ggaatggctt tataattatt aataatataa tgccatacaa
     4621 tgaggttttg ccagaagatt atcaagaagg atatatagta ttctccaaag gaatacctct
     4681 ttcgtctaaa actaaacgta aatattgggt tgttggtaaa aaaactgata aaaaacataa
     4741 gattgatgaa acagtagtga atgattatta taatgataaa aaccgaaata gcattaacct
     4801 gatagattat ctcaatagga ataaaaaaga atatacaaat ggaatccctt gtttcttttt
     4861 atatgaagat ggtcaagtga ctgcttttgg taataccgct cttttccgaa ttagctataa
     4921 aaactctatt ggaaagctcc ttcctgaaaa tcataaaagt gatgaactgg atatggcaga
     4981 agctcttttt ggaagagtta tgaaaaaaca agaagataca atcaatggaa gaatctattt
     5041 tgatgatgct gtagccaaag aggcagttgc ttttgaaaaa cctatttatc caaaaattct
     5101 tgccaatcca aaacctactt ccttccagtt atatttaaag caaccatcag atgttacttt
     5161 tgatactatc agacattatg atagtccaga tgcaaagctt agaggctata aacaatactg
     5221 gcataaaaaa ggtgataagt ttgaagaaga ctcacccgat tttgatcctc aaaaagatac
     5281 ccagcatact ttaatagctc ctataaagcc aggagctata tttgaaggtt ccattcgttt
     5341 tgagaattta agtgaagaag aattaggggc tttgcttttt gttttaaatt tacccgaagg
     5401 ctattgtcat aaaataggaa tgggaaaacc tcttggatta ggctcaatta agataacggc
     5461 aactctaagc atccttgacc ccaagaagcg ctatagcaat ctaactgata atggatatac
     5521 taatgatgat ccttctaaat atttggacgc ttataaagaa cttttaaaaa agcatcatat
     5581 tttaaataca gaaaatcctt gggatttaat gagatttaga gaacttaaaa aaatgcttga
     5641 ttttgctaat catcccgatg aatcaaaaac tgaatatatg cctttagatc aattcagaga
     5701 aagaggcgta ttgcctgaac cacttaaggt ctaataaata ataaagcccc gagatatatt
     5761 tcggggctca tttttctata ggtagattta tagctaaatc cataatctct ggcatagaaa
     5821 gtttcggatt ttgataccag atagttagag ctctttgaag ataaacacca atctctttac
     5881 cttctttaat ctgatattgt tccataaatt cagtgccagt tatatggaaa ggattagcat
     5941 ccatttcatt tttcagttct attgccagtt ttgacagagc agacttggtt cctgacctcc
     6001 atcctttata gttatccaca cattgtaata aaatcccgag ttcttcaaaa tcatcgttaa
     6061 ctttatgaat agcttcacga agatgactga gttccatttc agattttaga taaaactcat
     6121 ttagaaagac tcttatagca tagagtagat tatcaatgtg tctgttttgc ttattggaga
     6181 aatagcgatg ataacttatg agtggttcaa gattaacttt aaattctgag ttagaatccg
     6241 ttctgttcaa ataaaatggt aaacagagaa agcaaaatct tccctccaag ctttccaagg
     6301 tctggataag tttacttagt tcaggatgtt tcaaccagtt caaggtcttt tcatccagag
     6361 tgatattgtt aaattcagat aataaatgac tcattagctg aaaggattgt agcagatatt
     6421 gagctttaaa gaaagattta agtaggagaa ttttctcaag ctctcgggtt attctcaggg
     6481 gattaagttc ttcaagaagg aagctttttt tctggatttc agcacccgtt ttttcttcaa
     6541 tggtgaaatt atccagttct gcagctaagc gcactgctct cagaattctt atggggtctt
     6601 tcacaaagcc ctttccttcc gattcacagg ctctaattaa tctgctttct aaatctttta
     6661 tacctttttg ggtgaaatca agtaaatcac cgctatgaat agctatcatc aaggcattaa
     6721 tagtaaaatc tctggagcgt gcatcttctt ccaccgaggc aaaggtttta tggactttac
     6781 cattatccat ctcagtccga atttgcgtcg gagaaagttc cagtgaataa ccatctattt
     6841 tagtcattaa agtatcagtt ctctgatatc gcacaacttt tctgattaat cctttctctt
     6901 gcagaatatt agccagaagt ttatcgcctt cttccttttc cacacagata tccaaatcgg
     6961 ttctttctct atttagatac atatctcgga cacatccacc cgcaaaataa caacaatatt
     7021 ccagttctgt gccttttata gcaccataaa tcttctttcg tatctcttct aattctatca
     7081 tagcggattg cctgcagacc ctgagcacta atttcctaaa atatataggt attaattgcc
     7141 ttgaaaggat agatgatata ggattaaaaa tttaatattt ttcagcctgc agctcagact
     7201 tccaaaatct ccttttcttt acttttttca gcttcatcta tctttttaat ccattcatca
     7261 gtaatatcct ggatatcttt tagcatcttt ttctgttcat cttcgctgat ttcaccgtct
     7321 ttttccagtt ttttcacctg ttcattggct tctcttctga tattacgtat agcaacgcgg
     7381 gtatcttcag caatcttttt aagattctta gcgatatcct ttctcttttc ctcagttaga
     7441 ggttgaaaag gaagacggat aacatttcca tcattttcag gagttacccc cagattagca
     7501 gctaaaatag ctttctcaat atcagccaga gtggtcttat cccagggttg gacaataatc
     7561 attctgggtt ctggtatgga gatattacag agctgtttga tgggagtaga ctgtccataa
     7621 tagtttatac gaatgtcatc cagaacggaa gcactggcac gaccagttct aattttggaa
     7681 aattgatgca acatagcatc aaaagttttc ttcatctttt ctttacattc atctaaaatt
     7741 tcatccataa tatcctcttt tgtttccata gggaattaag cataaatata ggttccaaaa
     7801 tcaggttgag tgagagccat ttctattcca cctgctcggg tgagattaaa tatttttaaa
     7861 ggtatccgat tttccagagc taaagaaaat gctgtcatat ccatcactcc cagcttttgt
     7921 tccaggcatt gactaaaact ggcagaactg atgaattgag cattagaatc tacttttgga
     7981 tcggaagtgt aaagtccatc tactttggtg cctttcaata ccaaatctga ttgtaattct
     8041 attgctcgta aaatagcagc ggtatcagtg gtaaagaaag gatttccggt gcctccaccg
     8101 ataaagcata tttgaccagt ttccagcgct tctgaagctt tggaaggagt gtaaagttcg
     8161 gtgatattat ccattatcag acaggaaaaa gctctggctg gacaattttt tagatttagt
     8221 atctcagcta aataaagtga attttgaatg gtggctaaca ttccaatatg gtcaagcacc
     8281 actctatcaa gattctgatt tttccaggtt ccaccccgga atatatttcc accacccaga
     8341 actatagcta tactataacc tttttggcga actttaataa tttcatcagt taaccgatca
     8401 ataacctcat cgtcaaaacc atatccttga ggtcctgcca ggatttcgcc actgattttt
     8461 agcatcactc ggtgaatctt ttcaggctga aaaagcataa tatcacctca tttccgcttt
     8521 tatagaaaaa tttggattat tcttcgccca gagcgtaacg cacgaatctg gaaatctgaa
     8581 tattttctcc ggtctgagct atggtgctat tgattaggtc acgaacagtg acacctggct
     8641 cattaaccag ttcctgttcc agaagagcat tttcgctaca gaacttttta agggttcctt
     8701 ccactatttt ctctacgatt tctggctttt tcccttcgtt aatagcttta tttcttgcaa
     8761 tttctctttc cctttctatt attgcaggat ctatctgttc actggtgata gccagtggat
     8821 tggttgcagc aatctggagt gcaagttctt gagccagctt cttaaattgt tcagtacgag
     8881 ctacgaaatc actttcgcaa ttcaattcca gcataacgcc aagtctccca ttaaaatgga
     8941 tataagcatg gattaagcct tctttagtct ggcgacttgc cttactctct gctttagcaa
     9001 tacctctttc tcttagatat ttgatagccg cttccatgtc tccattagtt gcttccagtg
     9061 ctttacgaca ttctaaaatt cccacaccag ttctatctct tagttctttc acttgttttg
     9121 cagttatttc ttgcattgat actcctttgt aatggttaaa attgaatcag gacaggatta
     9181 gacctgttat ctctaagtgt taggcttccg gttgagcaat agtttcggtt aattcttcag
     9241 tcgtaactat ttcttcttcc tcttcttcgt ctttaggagg tgttgtcgcc atttcaatca
     9301 tctcaggtgt aaattcatct cctattacgg gcatctctaa agtcgcacct tcaccttcag
     9361 tagcgatatt tttaccttca agcacggcat ttgccattat atctgaaatt aattggatgg
     9421 cacgagtggc atcatcattt gccggaataa cataatccac caggtcagga tcacaattag
     9481 tatctaccat agcaatgata gggatatgca ggatgcgagc ttcatgaacc gcaatagctt
     9541 catgccctac atctacaaca aacatagcac caggtaaagc atccatatca cggataccac
     9601 ctaaatagag ctctatttta tcatgcatcc gcttcatctt tagctgttcc agcttggtat
     9661 atttgttgat agttccatct gcaacgatag attcatagta tttcattttc tcaatgctct
     9721 gtctgatagt ttgcatatta gtcaacattc cgccatacca gcgttgattg acatagaaaa
     9781 caccagcttt ttctgccgct tccttgatag ccgcttgagc ttgctttttc gtgcctacga
     9841 aaaggatgta ttcctgacga ctggctactt ctcttaaaaa ttgataggct tcattgatgg
     9901 catcaacggt ctgtttaagg tcaataatat gaatgccatt tctttttaag aagatatatt
     9961 tcttcatctt aggattccat ttataggttt gatgaccaaa atggactcct gcttccagca
    10021 attgtttcat tgtaactacg gacatcattt ctcctttcta aatattacgg tttattcttc
    10081 cgcaccagag atctaagcaa ttcagaggaa aagtatcctc accgccttgc aactctcaat
    10141 gcgtgtattt tgggttaatg gtagttctaa agtataccac ttaccttttt tagtattaac
    10201 gtttggaaaa ttggaatctt tttctggctt taggtctacc gcttttcttc cgttccacca
    10261 tcctggggtc acgggttaag tagcctcttg cttttaactg aggacgcaga ttttcatcaa
    10321 attccactaa ggcacgagca ataccatgac gaatagctcc cgcttgaccg cttagacctc
    10381 ctccagaaac attcacatac acatcaaaat tatcgctcaa accaagctcc tgcaagggct
    10441 gctctattat catagccaga gtttcgcgct gtagatattt cttcatctgc acttcattga
    10501 tgattctttt tcctgttcct ggcattaagc gaactcgtgc tactgcattc tttcttctgc
    10561 caactgcatt ataagtctgc atacttgatt ctccttaaag ttttaattcc acaggtttct
    10621 gagcactgtg tggatgttca ttaccagcat aaacttttaa ctttttaaac atagcatcac
    10681 ccagaacagt cttgggcatc atacctctaa ccgaatgttc aattatcttt gttggatttt
    10741 tctccagcat cctggaatag ggaatatctt tcaatccact gggatagcca ctaaaactat
    10801 gataaacctt ctggatgctc tttaaaccag ttactttaac cttttcagca tttatgacta
    10861 taacataatc ccccgtatca atattacgag cataatacgg tttgtgcttc ccgcaaagga
    10921 tagaagctat tttactggat aaacgaccca gaggttgatc cgtggcatct ataacatacc
    10981 aggtacgttc tatatcaccc ggtttgggag ttagggtctt catctcatct ccttacaact
    11041 tttagattaa aaatacaaga tttttgataa gcttatagtg tcaaggattt ttgc
//
LOCUS       DAORZF010000049        10955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_4542_length_10955_cov_369.256147, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000049 DAORZF010000000
VERSION     DAORZF010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 10955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10955
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4542_length_10955_cov_369.256147"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..339)
                     /locus_tag="PK015_06100"
     CDS             complement(4..339)
                     /locus_tag="PK015_06100"
                     /inference="COORDINATES: protein motif:HMM:NF039670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6150 family protein"
                     /protein_id="HOL34075.1"
                     /translation="MKKLLLLILMILMGFTCFAGSVYVMDSGKPDLVIWIAPYKSQAD
                     LCVYVAPYRSEARGKDEIWYFEKYRSSADIKIKITKYKSQADLKIYYVKYKSEAGWRK
                     SHPWMGRLH"
     gene            534..4148
                     /locus_tag="PK015_06105"
     CDS             534..4148
                     /locus_tag="PK015_06105"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOL34076.1"
                     /translation="MKKVLILIGLMMTCALAFSLTLNFSVNPNGWSKDSNANNTAPFL
                     EEGVPAMNYLPVRVLIPFGEKIENVQVILSELEIQRQQVQLDYVRKVQIISQPQPDNT
                     IPNPEIWNKDALFPAEDYKFLGTQMFCGCQIAVINIFPWKYNPVQKTIFTSNNITLQI
                     ETSWNHKLAEHSANFYAPARDYTDLTKIVLNPETINSYQNATAYRTHQSQSRLIDLSV
                     PKKMIIITNSSCSNYFQNYIDWQTTRNISTGLYLTSDIYSSYPGVDNPEKIRNFIIDA
                     YQTWSSTSTPLEYVILGGDDEIIPDRGCFCAVGETIDLRIPTDIYYSNLDGNWNADGD
                     QIWGEINDQVDYLPEVHIGRFPAETAIEFGNMTRKIQYYVNNDTYSNNLAIMFGENLN
                     NNPLTWGGDYKDDVATHIPDDYILTTMYQRDGTFSSLGVWNALNDGANVMNHMGHSNE
                     TSLLGQGIGSVEQLQNTEYGFLYTQGCYPAAFDQRTSGDGESIGEHMVTTSGGLFSFI
                     GNTRYGWYMPGSINGPSQFYDRQFFIGLYGANQPNLGKALTYSRLQNVNAAMNNDVMR
                     WCYYEMVLFGDPTIEVKYPDPTLPFLTLEGYSISDIDGDGDGSFNPGEILRIYPIIKN
                     SPEAGTAYNISVTLENLPQGIILLDGPQIISQLLPGASCDPSFYFRIQLPQDITYGTY
                     YLKILLDSSHPITGLSTGIRKFDLSFEITMVDGHFPWDCQFHTKSAPMVYDINNDGTL
                     DILYLNIFGDAYYINNNGEQFGGFTSSPQQDIMRSSAMGDLTGDGIPEIVYSSRTGKI
                     NAVTLDGTPVFSYDTGKQLIFTPVIATLSHSGTDKVIAHSLNGYVYAVDSNGSLLPGF
                     PVNLGSSIVTEIAATDINQDGLMEIIIGCQNGTLHILNSSGEEIPGFPVHIGENINGS
                     PTVLDNNNIVFGTNNHMILISPSGEILVNKPIVAAMASGPVVANISGDSALEIIFVTI
                     NGMLYAVDQNGNDINGFPLNVEDNFTVPPLIVNLDEDIQPEILLTSYQNSLYAYNNNG
                     TMLPGYPFITSFNGCTPGTVCDLDNNDMLKFVSGYSTGVVVMNLRSPETPIKPWITYR
                     GALNRQGSSACTGYVANSDPYIIPPVNRLEQNYPNPFRHNTTISYETKKEGKVSLSIY
                     NLRGQLIRTLQDEYIPGGKHSIVWDGKDSMGRTVAEGIYLCRLKTAETTLTRRMLFLK
                     "
     gene            4222..5145
                     /locus_tag="PK015_06110"
     CDS             4222..5145
                     /locus_tag="PK015_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HOL34077.1"
                     /translation="MPKVLIATEKPFAKEAVQKIKSEFDAAGYQQVWLENYTDPEELR
                     KAAADAEALIVRSDIVDEALFNAAPKLKIVVRAGAGYDNIDLAAATAHNVVVMNTPGQ
                     NSNAVAELALGLMIYMARGKFNGKSGTELAGKKLALFGFGYVGSRLAKMAKGMDMEVY
                     AYDPYISSEIMIQQGVTPLNKVEDIFTTGDYISLHIPATAETKKSVNWNLLRLVKDNC
                     ILVNTARKEIIDEDALIQALQEKNKFRYVSDIAPDCSEKLMAEFAERVFWTPKKMGAQ
                     TEEANTNAGVAAARQIIAFFEKGDTTFQVNK"
     gene            complement(5218..5484)
                     /gene="rpmA"
                     /locus_tag="PK015_06115"
     CDS             complement(5218..5484)
                     /gene="rpmA"
                     /locus_tag="PK015_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOL34078.1"
                     /translation="MAHKKGVGSSRNGRNSNPKYRGVKKYGNEYVIPGNIIVRQKGTK
                     IHPGKNVGMGRDFTIYSLAEGYVKFETKGAGRKYVSVVPIEKAE"
     gene            complement(5496..5813)
                     /gene="rplU"
                     /locus_tag="PK015_06120"
     CDS             complement(5496..5813)
                     /gene="rplU"
                     /locus_tag="PK015_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOL34079.1"
                     /translation="MYAIVEIKGFQYRAEENTVLSVPLLKTAKPGQTLEFDRVLLVRN
                     DDEIKVGQPVIEGAAVLAEVIGNGKGKKKIIYHAKRRKGYRVKKGYREQFTNVKVTGI
                     RVP"
     gene            6021..7298
                     /locus_tag="PK015_06125"
     CDS             6021..7298
                     /locus_tag="PK015_06125"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HOL34080.1"
                     /translation="MAIEKAESINFMMDEMWKWKMMVEKFDNMLKLYDYHEIHLPILQ
                     NRQLLQQGITAFMQKEEPEFISRQTLNINDQKNPENQLSLRPEGTISVLYRAASLYRK
                     GDVFRYYYQGPMFHRNREGVAQESSQLGVELVGTSNILSENEIISLGIRLLNEVGFYD
                     ATLKLNSYGCENCRRQFFIDVKTELKTHSDRYCPDCLQELGANPFAATHCENEHCQHS
                     LPPLLQISNYLCPECKARFEQIKKIQANLGNIYKVDPYLTKNFAYYNGTVFDFVVKQQ
                     DNELVVGDGGRYDYLSERLTGRKIPAIGFHLDLDLIFQLMEERNLFLMFDNDFSVYIY
                     AQSPELEMMVLQIAQELHSQNIITVLGTEEKPEDNLKQAQAHKCDLMITLREENIREG
                     KLLLYNLEHNEQTYIPLNRLLDAVQLVRKTLKK"
     gene            7312..7683
                     /locus_tag="PK015_06130"
     CDS             7312..7683
                     /locus_tag="PK015_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOL34081.1"
                     /translation="MQSVNTHLAPAAIGPYSQGILMNNTLFISGQLGIDPRTGDMPMS
                     FEAQANLVFNHLKAILDAASMGFAHVVKVTVYLDDMNHFSTLNEIYTRNFSAPFPARE
                     VVEVSRLPKDAMIEISVIAMK"
     gene            7686..8219
                     /locus_tag="PK015_06135"
     CDS             7686..8219
                     /locus_tag="PK015_06135"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOL34082.1"
                     /translation="MSIITRKGDEGKTSLYSGERVWKDNPRVKAYGTVDELDTFISDA
                     KNLITNPTYFQLLEEVQKILYRMMGELATKNNNYPEPITAEEVQKLTEQVQALEEKTP
                     LHGFVIPGSLPVSARLDICRTIARRAEREIVTLARKEQVPPEILQYINRLSDLLFMLA
                     RTLEAEAGVLNYLKPNK"
     gene            8239..8988
                     /gene="gpmA"
                     /locus_tag="PK015_06140"
     CDS             8239..8988
                     /gene="gpmA"
                     /locus_tag="PK015_06140"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HOL34083.1"
                     /translation="MYKIVLVRHGESIWNRENLFTGWTDVDLSEKGIEEAHSAGKRLL
                     QEGFEFDECWTSVLKRAIRTLWIILDEMDRMYLPIHNDWRLNERHYGALQGLNKAETA
                     AKYGEEQVKLWRRSYDVPPPPLDKDDPRYPGKDPRYAHLSPEQIPVGESLKDTCKRTM
                     PFWEEVIVPRLAAGRKILISAHGNSLRAIVKNLDGISDADIVNVNIPTGIPLIYEFND
                     DLSVIRHYYLASEEELAEAVSAVESQGKAKK"
     gene            9049..9219
                     /locus_tag="PK015_06145"
     CDS             9049..9219
                     /locus_tag="PK015_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOL34084.1"
                     /translation="MAVKIDKDICIGCAACIDVCPTGSLSMQEDKAVCDEGTCIDCGS
                     CIPVCPVQAISE"
     gene            9293..>10955
                     /locus_tag="PK015_06150"
     CDS             9293..>10955
                     /locus_tag="PK015_06150"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOL34085.1"
                     /translation="MKHNLLFLLLLIPGLILIPLNATDVSGNQSGTWTIANSPYNIIG
                     DVTVPNNSSLSIEPGVQIYAMGNFYLTVQGTLSAIGTVTDTIRFKSGQNDPNALWKGI
                     RLENETQTSYISYCYLEKAEYGVNSVNSPVQITHSRFSYNQKAMQIFAIGASNPANVT
                     VSENIIERSIQNGIMVVQNSDLVIENNEIRYNGTGSQYMAAIQLSNQSSNGSNSPLIQ
                     NNHIHHNFKQGITAWDLVAVNAIQPQILNNIIENNLTGIYLLNSSGYIADNIIRNNFI
                     PGDANSGAGVMVTGATSEPYFERNQIYGNFTGFYLGQNAKPCLGNLSIYHSWAQGENA
                     IYNNIDESNTLHSVYTYSYTNPSIVIYAENNYWGTTDPAEIAAGILDHMDNPSLPTVD
                     FEPFLLVYNTTTVTGQITYNGNFNLINHRLQMIGVDSGEILAEEFINPQYPFSHTSLL
                     TEPFYVVVLADVAGIDQTFYGTPGGLLQPQTFNPSPNQTINVGSIEISEIPPTIYQTI
                     GTPMMIGTHNCYPLYHRFFVYHWDYINWLYEAGDYLYLARHERYNP"
BASE COUNT         3435 a         2066 c         2088 g         3366 t
ORIGIN      
        1 ccattaatgc aatctaccca tccagggatg ggattttctc catccggctt cacttttata
       61 tttcacataa taaattttga ggtctgcctg agatttgtat ttagttattt ttattttaat
      121 atccgctgag cttctatatt tttcaaagta ccagatttca tccttccctc tggcttcgga
      181 acgataaggt gccacataca cacataaatc tgcttgagat ttatacggag ctatccagat
      241 taccaaatcc ggttttccac tgtccattac atagactgat ccagcaaaac aggtgaaccc
      301 cattaatatc attaatatga gcaataaaag ctttttcatt atgcctacct tttatctctc
      361 ttttacctta atccctataa atttttatta gcttctcaat acttttttgt caatgaattt
      421 ctgaagaccc tgttgaatta aacctgaact taataacagg agaaaatact tgactaagga
      481 tggctctgga tattcataga aataataaaa tattttatac taggaaacat acaatgaaga
      541 aagtgcttat attgataggc ttaatgatga cttgtgctct cgcattcagt ttaacgctga
      601 actttagtgt taatcccaat ggctggagta aagattcaaa tgcgaataat actgctcctt
      661 ttctggagga aggtgttcca gcaatgaatt atcttcccgt ccgggtgcta ataccttttg
      721 gagagaagat agaaaatgtg caggtgattc tttctgaact tgaaattcag cgccaacagg
      781 tgcaattgga ttatgttcgt aaagttcaga ttatatctca acctcaacct gataacacca
      841 tacccaatcc cgaaatctgg aataaagatg ctctcttccc cgctgaagat tataagtttt
      901 taggcactca gatgttctgt ggttgtcaga ttgctgtgat aaatatcttt ccctggaaat
      961 ataatcctgt ccagaaaaca atcttcacaa gcaataatat aacccttcag atagaaacct
     1021 cttggaatca taagttagcc gagcactcag ctaactttta tgctcctgca agagattaca
     1081 cggatttaac taaaattgtg cttaatccag aaactataaa ctcctatcaa aatgctacag
     1141 cgtatcgcac tcatcaatct caaagtagac ttattgattt gagtgttcct aaaaagatga
     1201 ttataattac taatagttcc tgttctaact atttccaaaa ctatatagac tggcaaacca
     1261 cacggaacat aagcacgggt ttatatttaa cttccgatat ctatagctcc tatccaggag
     1321 ttgacaatcc tgaaaaaata cgtaatttca ttatagatgc ctatcaaacc tggtcaagta
     1381 cctctactcc tttagaatat gttatcttgg gaggagatga tgaaataatc cctgatagag
     1441 gctgtttttg tgcggtcgga gaaactattg atttaagaat accaactgat atctattaca
     1501 gcaacttgga tggcaactgg aatgctgatg gagatcaaat ctggggtgaa attaatgacc
     1561 aagtagacta ccttcccgaa gtgcatatag gaagatttcc tgcagaaact gctattgagt
     1621 ttggtaatat gacccgtaaa atccagtatt atgttaataa tgacacctat agtaataact
     1681 tagctataat gtttggagaa aatctaaaca acaatccttt aacctggggc ggagattata
     1741 aggatgatgt agccactcat atcccggatg attatatact gaccactatg tatcagcgag
     1801 atggaacctt cagttcactc ggagtatgga atgctcttaa tgatggagca aatgtaatga
     1861 atcatatggg acactccaat gaaacatctt tgctgggaca gggaattggt tctgtagaac
     1921 aactgcagaa tacggaatat ggatttttat acacgcaggg atgctatcca gcggcatttg
     1981 accaaagaac cagtggtgat ggagaaagca ttggagaaca tatggtaact acctcgggtg
     2041 gacttttttc atttataggt aacactcgtt atggatggta tatgcctggt agcattaatg
     2101 gtccttccca gttttatgac cggcagtttt ttataggatt gtatggggct aatcagccta
     2161 atttaggcaa ggctttaacc tattctcggt tgcagaatgt caatgcagca atgaataatg
     2221 atgtaatgcg ctggtgctat tatgaaatgg tgctttttgg agacccaacc atagaagtta
     2281 aatatcctga tcctacgctt ccttttctta ctctggaagg ttatagcatc tctgatattg
     2341 atggagatgg tgatggcagt tttaatcctg gagaaatctt aaggatatat cccattatca
     2401 aaaattctcc tgaagcagga actgcttata atatcagtgt gactctggaa aatctacctc
     2461 agggtataat tcttctggat ggacctcaaa ttatttccca gttattacct ggagcatctt
     2521 gtgacccctc tttttatttc agaattcaat tacctcagga cataacttat ggaacttact
     2581 atctgaagat tttactggat tccagtcatc ctattaccgg tctaagcact ggaataagaa
     2641 aatttgacct cagctttgag ataactatgg tagacggaca tttcccctgg gattgccagt
     2701 ttcatacaaa atctgcacct atggtttacg atattaataa tgatggaact ctggatatcc
     2761 tctatcttaa tatattcggg gatgcatatt atataaataa taatggagaa caatttggtg
     2821 gatttacctc ttctcctcaa caggatatta tgcgaagttc agcaatgggt gacctcactg
     2881 gagatgggat accagaaata gtctattcca gtcgcaccgg aaaaattaat gctgtaacct
     2941 tagatggaac tcctgttttc agttatgata ctggcaaaca actcatcttt acccctgtga
     3001 tagccactct tagccattct ggtacagata aggtcattgc ccattcttta aatggatatg
     3061 tctatgctgt agattctaat ggctctttac taccaggatt tccagttaat ttaggtagct
     3121 ctatagtgac agaaattgcc gcaacagata ttaaccagga tggcttaatg gaaataataa
     3181 tcggttgtca gaatggcact ttacacatct taaactcttc tggagaagaa attccaggat
     3241 ttcctgtcca tataggcgaa aatataaatg gttctcctac cgttctggat aataacaaca
     3301 tagttttcgg aactaataat catatgatac taatttctcc cagtggagaa atcctcgtca
     3361 ataagccaat tgtagctgct atggcatctg gaccagtagt agcaaatatc tcaggagact
     3421 ctgctctgga gattatattt gtcactatca atggtatgct ttatgccgta gaccagaatg
     3481 gaaatgatat aaacggtttc ccgcttaatg tagaagataa ttttactgtt ccacctttga
     3541 ttgtgaattt agatgaagat atccagcccg agatattgct aaccagctat caaaattctc
     3601 tatatgccta taataataac ggcacaatgt taccgggtta tccttttata acttctttta
     3661 atggttgcac tcctggaact gtatgcgatt tggataacaa tgatatgctt aaatttgtta
     3721 gtggttattc cactggtgta gtggttatga acttacgcag tcctgaaaca ccaattaaac
     3781 cctggattac ctatagagga gctttgaatc gtcaaggtag ctctgcttgc acaggttatg
     3841 tggcaaattc agacccttat atcattccac cagtaaatag actggaacaa aactatccca
     3901 atcctttccg tcataatact acaatctcct atgaaacgaa gaaagaggga aaagtaagtc
     3961 tttccattta taatttaaga ggtcagctaa tacgcacttt acaggatgaa tacatccccg
     4021 gaggaaaaca tagtatcgta tgggatggta aagattcaat gggaagaact gttgcagagg
     4081 gaatatatct ctgcagatta aaaacagcgg aaacgacttt aactcgtcgt atgcttttct
     4141 taaaataatc tgggacttga cagatcgctt tttcgggtta atgatatagt aagaaatata
     4201 aaaatataag gagttgcaaa aatgccaaaa gtattgattg ccaccgagaa accctttgct
     4261 aaagaagcag tacaaaagat taaaagtgag tttgatgccg caggatatca gcaggtatgg
     4321 ttggagaact atactgaccc ggaagaactt cgtaaggcag ctgccgatgc ggaagctctg
     4381 attgtgcgta gtgatattgt ggatgaagct ttatttaatg ccgctcccaa actaaagatt
     4441 gtggttcgtg ctggtgcagg atatgataat attgaccttg cagctgcaac tgcacataat
     4501 gtagtcgtga tgaatacccc gggacagaat tctaatgctg tagcggaact ggctctcggt
     4561 ctaatgattt atatggctcg gggaaagttt aatggaaaaa gcggaactga gcttgcgggc
     4621 aaaaaactcg ctctattcgg ttttggctat gtaggaagtc gcttagctaa gatggctaaa
     4681 gggatggata tggaagtcta tgcctatgac ccctacatca gttcagaaat aatgattcaa
     4741 caaggagtca ctccactgaa taaagttgaa gatatcttta ccactggaga ttatatctct
     4801 cttcatatac ctgctactgc agagaccaaa aaatccgtaa actggaattt actcaggctg
     4861 gttaaagata attgcatctt ggtcaacacc gctcgcaaag aaatcattga tgaagatgcc
     4921 ctgatacaag ccttacaaga aaagaataag ttccgctatg taagtgatat agcaccagat
     4981 tgcagtgaaa aacttatggc agaatttgct gaacgagtct tctggactcc aaagaaaatg
     5041 ggagctcaaa cagaagaagc taataccaat gcaggtgtgg cggctgcgcg tcaaattata
     5101 gctttctttg aaaaaggcga caccactttt caagttaaca aataactcat cattctaacc
     5161 ccataaaaat aaccccggta tcaaaaccgg ggttatttta taatagattt atttttttta
     5221 ctctgctttc tctatcggaa ccacagaaac atattttctg cctgctcctt tagtctcaaa
     5281 cttcacatac ccttctgcta aactgtaaat agtgaaatct ctacccattc ctacattttt
     5341 ccccgggtga atctttgtcc ctttctgacg gactattatg tttccaggaa tcacatattc
     5401 attcccatac ttttttacac cacgatattt gggattgcta tttctgccat ttcggctact
     5461 tccaacacct tttttatgtg ccataatatc tctccttagg gaaccctaat gccggttact
     5521 ttaacattag taaattgttc acgatatcct ttcttaactc tatagccttt tctgcgcttg
     5581 gcatggtaaa ttatcttttt cttacctttt ccattaccaa taacttctgc cagaactgct
     5641 gcaccttcta ttacaggttg tcccacttta atttcatcat cgtttctaac gagtaaaacc
     5701 ctatcaaact caagagtttg acctggtttt gccgtcttca gcaggggaac acttagaaca
     5761 gtgttttctt ctgccctata ctgaaatcct ttaatttcaa cgatggcata catttttact
     5821 cctaaatttc tttattcttt aaccttcttt ataggacaag aatataagtc aagagaaatg
     5881 tttttatctt ctctgaaaag gataaaatcc tccctgggga aaggacctat aaacatgtgc
     5941 acatttaaat accacttcct gctttatttt cattgacaga aatactaatt ttctctatga
     6001 agaaaagtaa ggagaaatat atggcgatag aaaaagctga atctatcaat tttatgatgg
     6061 atgaaatgtg gaaatggaaa atgatggtag aaaagtttga taatatgctc aaactttatg
     6121 actatcatga gattcatctt cctatattgc aaaatcgcca gttgttacaa cagggaataa
     6181 ccgcctttat gcaaaaggaa gaacctgagt tcatttcccg tcagaccttg aatatcaatg
     6241 accaaaaaaa tccagaaaac cagctcagtt tacgcccaga aggtacgatt agtgttttgt
     6301 atcgtgcagc ttcattatat cggaaaggag atgttttccg ctattattat caaggtccaa
     6361 tgtttcatcg gaatcgtgaa ggtgtagctc aagaatcttc tcaacttgga gttgagctgg
     6421 tgggaacttc caatattctc tcagaaaatg aaattattag tctgggaatt agattattaa
     6481 atgaagtggg cttttatgat gccacattaa aactcaatag ttatggatgt gagaattgcc
     6541 ggagacagtt ttttatagat gtgaaaacag agctgaaaac tcattctgat agatattgtc
     6601 cagattgtct gcaagaactg ggtgctaatc cttttgctgc cactcattgt gagaatgagc
     6661 actgtcaaca tagtcttcca cctctgttgc aaataagtaa ctatctctgt ccagaatgta
     6721 aagccagatt tgagcaaata aagaaaattc aggctaatct ggggaatatt tataaagtag
     6781 acccctattt aaccaagaat tttgcttatt acaatggaac cgtatttgat tttgtagtca
     6841 aacagcaaga taatgagtta gttgttggcg atggaggcag atacgattat ctttctgaac
     6901 gcttaactgg aagaaagatt cccgctattg gtttccatct ggatttagat ttgatattcc
     6961 agctgatgga agaacggaac cttttcttga tgtttgataa cgatttttcg gtctatattt
     7021 atgcacaatc tcctgaactg gaaatgatgg tattacaaat tgcccaggaa cttcattcgc
     7081 aaaacattat aaccgtgctg ggcacagaag aaaaaccaga agataattta aagcaagctc
     7141 aagcacataa gtgtgattta atgattacct tgcgagaaga aaatattaga gaaggaaagt
     7201 tgttactcta taatttggaa cataatgaac aaacttatat tcctctgaat cgccttctgg
     7261 atgcggttca gttagttcgt aaaaccttaa aaaaataata ggagttatct tatgcaatcc
     7321 gtaaacactc atctcgcacc cgctgccatt ggaccttatt cacaggggat attaatgaat
     7381 aatactcttt ttatctccgg gcaattgggg atagaccctc gcaccggtga tatgcctatg
     7441 agctttgaag cccaagctaa tcttgttttt aaccatttaa aagctatatt agatgcagcc
     7501 agtatgggtt ttgcgcatgt ggtaaaagtc accgtctatc tggatgatat gaatcatttt
     7561 tctaccctaa atgagattta tacgcgtaat ttcagtgctc catttcctgc tcgggaagtg
     7621 gtagaagtct ctcgtcttcc caaagatgca atgatagaaa tctccgttat agcaatgaaa
     7681 taaatatgag tatcatcacc agaaaaggag atgaaggtaa gaccagctta tatagtggcg
     7741 aaagagtctg gaaagataat ccgcgggtta aagcctatgg aacagtagat gaactggata
     7801 cctttattag cgatgccaaa aatttaatta ctaatccaac ctattttcag ctgctggaag
     7861 aggtccagaa gattctatac agaatgatgg gtgagttagc cactaaaaat aataattatc
     7921 cagaaccgat aactgctgaa gaagttcaaa agttaacgga acaagttcaa gctctggaag
     7981 aaaaaacacc tctgcatggt tttgtaatac caggttcttt accagtttcc gcaagattgg
     8041 atatatgtcg cactattgct cgtagagcag aaagagagat agttactctt gcccgaaaag
     8101 agcaagtgcc ccctgaaatt ttacaatata taaacagact ttcggatctc ctgtttatgt
     8161 tagcccgcac tttagaagca gaggcaggag tccttaacta tttaaaacca aataaataaa
     8221 ttatagagga gcaacactat gtataaaata gtattagtta gacatggtga aagtatctgg
     8281 aaccgggaga atctctttac gggatggacg gatgtagacc tctcagaaaa gggaattgaa
     8341 gaagctcata gtgctggtaa gagattattg caggaaggat ttgagtttga tgaatgctgg
     8401 acttctgtgt taaaaagagc tatcagaacg ctctggatta tcttggatga aatggatcgg
     8461 atgtatctac ctatccacaa tgactggcgt ttaaatgagc gtcattatgg tgctttacag
     8521 ggattgaata aagcagagac agcagcaaaa tatggtgaag agcaggtgaa actatggcgc
     8581 agaagttatg atgttccgcc tccaccctta gataaagatg atccaagata tccaggaaaa
     8641 gacccgcgtt acgctcatct ttctcctgaa cagattcctg tgggtgaatc tctaaaggat
     8701 acctgcaaaa gaactatgcc tttctgggaa gaggtcattg tcccacgttt agcagcagga
     8761 agaaagatac ttatttctgc tcatggtaat agccttagag ctatagtgaa aaatctggat
     8821 ggtatcagtg atgcggatat cgttaatgtt aatattccca cgggaatccc tctaatttat
     8881 gaattcaatg atgaccttag tgttatccgt cattattatc ttgccagtga agaagaatta
     8941 gctgaagcag tgagtgcagt ggaaagtcag gggaaagcta aaaagtaaat tttccttgac
     9001 gaagagacta ttctcaaaat aataaataaa aaaatatagg agttagaaat ggcagtaaag
     9061 attgacaaag atatttgcat cggttgtgct gcttgcatag atgtctgtcc cacaggttct
     9121 ttatctatgc aagaagacaa agccgtttgt gatgaaggca cctgcataga ttgtggttct
     9181 tgcattccag tctgtccagt gcaagctatt tcggaataaa cttataaaca ggtcattatc
     9241 gcggaagagg gtgtttcttc ttccgcttta ttttctttag gaggaggata aaatgaaaca
     9301 taacttatta tttttgctac ttttaatccc tggactcatc ctgattccct taaatgctac
     9361 agatgtgagt ggcaatcaat caggaacctg gactatagct aatagccctt ataatatcat
     9421 cggtgatgtt acagttccca acaattcttc tttatccata gaacctggtg tacaaattta
     9481 tgctatgggt aatttctatc tcaccgtgca gggtacttta agtgcaattg gaaccgtaac
     9541 cgataccatc cgatttaaaa gcggacaaaa cgatcctaat gctttatgga aaggtattag
     9601 gttagaaaat gaaactcaaa ccagttacat atcttattgc tacctggaaa aagctgaata
     9661 tggtgtaaat tcagttaatt caccggtaca aataactcat agccgttttt cctacaatca
     9721 gaaagcaatg caaatctttg ctatcggagc atctaatcca gcaaatgtaa ctgtttcgga
     9781 aaacattatt gaacggagca tccagaatgg tataatggtt gttcaaaaca gtgatttagt
     9841 gattgaaaat aatgagattc gttataatgg cacaggttct caatatatgg cagctattca
     9901 gctttccaat caatcttcca atggttcaaa ctcaccttta attcagaata atcacattca
     9961 tcacaatttt aaacagggta taactgcctg ggatttggtg gctgttaatg ccattcaacc
    10021 tcaaatcttg aataatataa ttgaaaacaa tctaaccggc atctatctat taaattcttc
    10081 tggttatatt gcagataata tcatcagaaa taactttatc cctggagatg ccaattctgg
    10141 tgcaggagtg atggtaactg gtgctaccag tgaaccctat tttgagcgta atcaaatcta
    10201 tggtaatttc acgggctttt atttgggaca gaatgccaaa ccctgtttag gcaatctttc
    10261 tatctatcat agctgggctc aaggtgaaaa tgccatctat aataatattg atgagtccaa
    10321 tactcttcat agcgtttata cctattctta taccaatccc agtattgtaa tttatgccga
    10381 aaacaactac tggggcacca cggatcctgc agaaattgct gcaggtattc ttgatcatat
    10441 ggataatccc tcacttccta ctgttgattt tgaaccattt ttgcttgtat ataataccac
    10501 cacggttact ggtcagatta cctataatgg aaactttaac cttataaatc atcgtctcca
    10561 gatgatagga gtggattcag gagaaatctt agccgaagaa tttattaatc ctcagtatcc
    10621 tttctcccat acttccctgt taaccgaacc tttttatgtt gttgttttag cggatgtagc
    10681 aggtatagac caaacatttt atgggacacc tggtggttta ttacaacctc aaaccttcaa
    10741 tcccagtcct aatcaaacga taaatgttgg ttctatagaa ataagtgaaa ttcctcctac
    10801 catctatcaa actattggaa ccccaatgat gataggcact cataattgtt atcctttata
    10861 ccatcgcttc tttgtctatc attgggatta cattaactgg ctgtatgaag caggagatta
    10921 tctctattta gctcgtcacg aacgctataa tcctg
//
LOCUS       DAORZF010000050        10472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_4789_length_10472_cov_355.472689, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000050 DAORZF010000000
VERSION     DAORZF010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 10472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10472
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4789_length_10472_cov_355.472689"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            345..1496
                     /locus_tag="PK015_06155"
     CDS             345..1496
                     /locus_tag="PK015_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOL34086.1"
                     /translation="MDDKLYKIFEEVKRKSECTLGPRAYRDADAKRMRPEEPDDMRTQ
                     FAVDRDRILYSGAYRRYHGKTQVFSFTNLIDEEMTNRNLHIAYVSQISRTIGKYLGLN
                     TELIEAIALGHDLGHPPFGHDGEIALSDACVRHGIGHFHHNIESLHIVDHISRKGRGL
                     NLTFPVRDGIISHDGEVHNTILYPEKDKDEARIQEYIRLKKEGMELDWMPATLEGCVV
                     RITDTIAYIGQDIEDAIRFNLLKREDLPQKVTDKLGNTNSQIIDTLIRSVIINSYGQD
                     WIAFDEETSYYLLELKKFNYENIYTNEKVKKSNKIIYRTMGIMFDKYLEDIEKNNRES
                     KIFKHFLDHKSPEYREGFSPAEQVRDFIATMTDRYYNEEIKDYLLPWKW"
     gene            complement(1514..2302)
                     /locus_tag="PK015_06160"
     CDS             complement(1514..2302)
                     /locus_tag="PK015_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOL34087.1"
                     /translation="MKKNASQYYLPILFFIVIILIWQYLSSRTVVAFWILPSPKEVIQ
                     VFKEFPELIWSHMKPTIIESVSGLLLATILGVLTALAMHSSKIIKQIFYPYLVVSQTI
                     PIIAVAPLIVLWFGYGIPAKIFVVVLMCFFPIALGLYDGLKQVSQEQIRLLKSMGASD
                     WKIYRYLKIPASLPAFFTGLKLAATYSVMAAVIGEWLGGNAGLGIYLTRSTKSFRTAS
                     VFAAIIVIILLSLAMFGIVALIDRILLSWQYKKIEEYEDISSFN"
     gene            complement(2299..3234)
                     /gene="murB"
                     /locus_tag="PK015_06165"
     CDS             complement(2299..3234)
                     /gene="murB"
                     /locus_tag="PK015_06165"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOL34088.1"
                     /translation="MMLDQLIKEQLPTLIEKGNIRFDVPLAPYTSFKIGGPAEVFCQP
                     QNIEELIDILKVALSFNIPYFILGNGSNLLISDKGVDGIVIQTSALNQLKHEGNFIEA
                     YCGATLKDLCYFAKEEGLSGLEFASGIPGTVGGAVFMNAGAYGSEIKDVLLSSDYLEP
                     NLDSLNSLNPIKHLNANEHNFAYRRSIFQEKNLIHIKSVFQLQPEDPNIIAEKMRSFE
                     EKRKEKQPLDLPSAGSVFKRPEGFYTGKLIEQCGLRGYQIGKAAISEKHCGFIVNLGG
                     ATAADVLALIKYVQETVYDRFGVKLETEIRFLGKE"
     gene            complement(3234..6080)
                     /locus_tag="PK015_06170"
     CDS             complement(3234..6080)
                     /locus_tag="PK015_06170"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HOL34089.1"
                     /translation="MEKEELAGLKNNLDFVAIDIETTGLDPSRDEIIELAATRFKSGK
                     IEDQFSTLVKPHRGMPKYIEFLTHINPDDLSTAPEAKIALQDLFSFVGDSVLVGHNVP
                     FDIGFINYHSALNKGAVLTNKFWDTQEISRVYFPFVSDHKLSTMLKFFHITPEAAHRA
                     TADAEASGLLLLAMTEHIIKHHSMMTNARLLDLSKQAQLNNSLYDYLHLLVEYQRSYV
                     LKGEHTTPLDYAKPNVIENDIPFHNISLDEVFNSEGLLSQKFPHYEFRSGQLEMANKV
                     KEAFQNTKHLVIEAGTGVGKSFAYLVPAMIFAHKNKTRVVVSTNTKNLQDQLFYKDLP
                     QLKKMLPLPFKASLVKGRENYVCERRWNDMLMEQSTALTPYEAQALLYLYIWKQLTKS
                     GDISENSSFDRKRFNIIWKRICSDRYQCMGRKCPHSGKCYVLNLRKHIENSTIVVTNH
                     SLLLTDLRMENTTLGKYDYLVVDEAHNLMDSASKNLGFEVGYQDLVNLFNQFAPATKR
                     KNVGLLNQLDFMLKRSVIPETSNEQIQMITKSLSEQISTMRKISLELFTEAQDLCQQA
                     DSYSKLRIKNPEDFPHLYESLDKLNNQWHSFLKQLSNLADTFSMLNSKQVPNYDTLNE
                     SINGLVKRSVDIQVGLDILENPDLMNNALWIENDLRPDPKTPVSTFCYAPVDVSLQLN
                     EMLYKNIPSIIFTSATLALRQSFKYFFTQSGLSLIPPEQLDEYIVDSPFDYDKQSLLM
                     ISSFLPEPKDRFFINQALGCVEQLVLSVDVGTMILFTNYKDLNSVYDYLSEPLYHRKR
                     PLFAQGKTYSRSSMLEEFKSYKNAVLLGTNSFWEGVDIQGDSLSMLILFKLPFQVPSE
                     PIVEALIDKLNTENKDSFMHYMLPNALLKLRQGFGRLIRSKEDRGIVLIMDSRVSNKY
                     YGKYFKEVLPAKCMEIKSELELLNEVIRFFNVSK"
     gene            complement(6095..10378)
                     /locus_tag="PK015_06175"
     CDS             complement(6095..10378)
                     /locus_tag="PK015_06175"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOL34090.1"
                     /translation="MTEGLTQKLHNYISQRFSLSKVLNLLLDKSHSHLIEITGKAGSG
                     KSYLIKPLMETLQNTYEKVYFYSPHPLYLNQFSLLLPIISDVEPDALEEFYQEYNQQY
                     RTGRKYDFFYYLTEQLNRHNLLTPRAIIIDDGDILDKYSRDYLQYLVQYAEDSGIQIV
                     FLSQQRLFPFAHWEIVPPLTVEDVQNIIQLAFPEAVENVGKESEILQKIVEGNMLILE
                     KIFNDMQLGENGTEFDLTPFLDKNYDPLQIYFETLENLPENQKELLLSMFILDQKFNE
                     DNIKNISGKKNIKKDLEKLYNAGLIEHPDLDWVVQKKVSMMIWLEQHPEALSSPLIQK
                     LLDNLAEDKSEEQIKVKILMQMNKYEASAFEAVIKELENLSDNDSLLQLYQYQLQHTK
                     KPDIQMLIHKNIGLAYANSNKKDEAIEYLRQSLHICTENFLPAEEIVYLLASNLFAVN
                     STAFALEIINKYSPETIDPYWKLKILLLKADILGETEDYDSALEELEKISHQSTSIND
                     KDKRLLLQGEARKIKGKILYYINEWEQSEENFKEAEKFFSLANDHSGLAAVYNNLAVL
                     YMFQGDWDRSEQYFLKSLALEKEHFNLNGISICYNNLGGLMDDKGDGQKSLMYLEEAL
                     KLQKMLNEPYNITNIYNNIGITWMDHNEYDKAEEALKNSLQTAIDFGFYRNIIASLNN
                     LGALYFKKGDWAQSIALYEQAIKKSEENNFTEGLLRSFNNLGEVYEKQDELTLAYDLY
                     FKGLELLPSVNDDFIKADLYGNLGSVLTKLHKFKEAYTYLVESLDFFKSINARDKIIE
                     GYQKQAYYFILTRNYDSAEYYLTEAMNMASEGNRLFELGKIHYLRALLERKNLESAKE
                     HLHQAIELFVETSNNYELSLANYELAEVLFDLKEWEAALEILKTNKKIIQKYGSIKLL
                     EKNDILMQKISREYSSQLRDVKFEENLLNQFYEITQRLNTISDLDVLIEQTLSGLIDI
                     SEADGGILCLHRSETVPDSWEYKIFQNFSSENKYYDIFMNLCNMAYDTNSIKDFKQPH
                     FAPSYNHIIVLPLFIRKNVLGTVLLFAQSGSHYFSERIINLLSALTNQAIITIENIRS
                     AGLEKTHNSIREQLNMGNVYSDIIGQSPEMLKIFDIIEKVKDTPTTVLLEGPSGTGKE
                     LLARALHYRSNRKNKAFVAQYCGALPETLLESELFGHVKGSFTGAAYDKKGLFEIADG
                     GTFFLDEIADITPSTQAKLLRFLQEGEIKRVGSTKTEKVDVRVVCATNVPLEERVKSG
                     AFRLDLYYRLNVIRIEVPPLKNRTGDIPLLAIHFLDKYNTRMGKNVQGFTDEAMKMLE
                     QYDWPGNVRQLENEIERAVTLAEDASFIRGTDFSDEVRRYIENQKTISILSGRKTLKD
                     AMEELERKMIQDAMEAFDWNQTQAAKELGISRQGLIQKLKRYNLYKEGE"
BASE COUNT         3219 a         1887 c         1932 g         3434 t
ORIGIN      
        1 gacgattact tagctgaagt aataggaaag agccaagaga gagcttagat atttaaaaat
       61 gaatttgttt gataaaatac gtgattaaaa gggagtatta taaatgattt tatgaaaata
      121 gggaaaacac ctgttcatat tctaacacag aaattaagac cgcttatgct attgatatta
      181 aacgggtaac cttgtgaaaa agtaggtcac taccatcact gacacctctt atgtttataa
      241 aaagtatgct tatcccagcg ttatgatttg gttttacaat gtatccagtt atattgttat
      301 aatagatgaa taatacagta atgcattaag gcagggataa taaaatggat gataaattat
      361 ataagatatt tgaagaagta aagcgaaaat cggaatgcac acttggtccg agagcctatc
      421 gggatgctga tgctaaaaga atgagaccag aagaaccaga tgatatgcgc actcaatttg
      481 ccgtagatag agataggata ttgtatagtg gtgcatatcg caggtatcat ggtaaaactc
      541 aggttttttc ctttactaat ttgattgatg aagagatgac caatcggaat ttgcacatag
      601 cttatgtttc tcagatttct cgcactattg gcaagtattt aggattgaac acagaattaa
      661 tagaagctat tgcgttgggt cacgatttag gacatccacc ttttggtcat gatggagaga
      721 tagctttatc tgatgcttgt gtgagacatg gtatagggca ttttcatcat aatattgaat
      781 ctttgcacat tgtagaccat atttctcgta aaggaagagg attgaattta acctttcccg
      841 taagagatgg cattatttct catgatggag aagtgcataa tacgatttta tatcctgaaa
      901 aggacaaaga tgaagctcgg attcaggaat atataagatt aaagaaagaa ggaatggaac
      961 ttgattggat gccggcaact ctggaaggat gtgtagttag aattacagat acgattgctt
     1021 acatcggtca ggatattgaa gatgccattc gttttaatct tttgaaaagg gaagatttgc
     1081 cccaaaaggt tactgataaa ctgggtaata ccaacagtca gattatagac actttgatta
     1141 gaagcgttat tattaacagt tatggacagg attggatagc ttttgatgaa gaaacttcct
     1201 attatcttct ggaactgaag aaattcaatt atgaaaatat ttacaccaat gaaaaagtga
     1261 aaaaatccaa taaaatcatc tatcgcacta tgggaataat gtttgataag tatctggaag
     1321 acattgagaa aaataaccgg gagtccaaga tttttaaaca ttttttagac cataaatctc
     1381 cagaatatcg ggaaggtttt tctcctgctg agcaggtgcg tgattttatt gctactatga
     1441 ctgaccgcta ttataatgaa gagattaagg actatttatt accttggaaa tggtaaagtt
     1501 attctttagc taattaatta aaggaggata tatcttcata ttcctctatt ttcttatatt
     1561 gccaggagag taggattcta tctattaatg ccactattcc aaacatagct aaactcagta
     1621 ggataatgac tattatagca gcaaagacac tggcagtacg gaaggacttg gtagagcgag
     1681 taagataaat tccgagacca gcatttccac ctaaccattc accaattaca gcagccataa
     1741 cactataagt ggcagcaagt tttaatccag taaagaatgc aggtaaggaa gcgggtattt
     1801 tcaaatagcg ataaatcttc caatcgcttg cacccattga ttttaaaagt ctgatttgtt
     1861 cttgtgaaac ctgttttaaa ccatcataca gacccaaagc tatagggaaa aagcacatca
     1921 atactacgac aaagatttta gctggaatgc cataaccaaa ccagagaact attaggggtg
     1981 ccacagcaat gatgggaatg gtttgtgaaa caaccagata gggatagaaa atctgtttaa
     2041 taatttttga actatgcata gctaaagcgg ttaacactcc caaaatagtt gccagaagta
     2101 gtccagaaac gctttcaatg atagttggtt tcatatgaga ccagattaat tcaggaaatt
     2161 ctttaaagac ctgaataacc tctttaggtg agggcagaat ccagaatgca acaacagttc
     2221 tggagcttaa gtattgccag attaggatga ttactatgaa aaacaggatg gggagataat
     2281 attgagaagc gttctttttc attctttacc caggaatcgt atttcagttt ccagcttaac
     2341 tccaaatcta tcatagacag tttcttgcac atatttaatt aaagctaaaa catcagctgc
     2401 agtagctcca cccagattta cgataaaacc acagtgtttt tccgagattg ccgcttttcc
     2461 tatttgataa ccacgtaaac cgcattgttc tatcaattta ccagtataaa atccttctgg
     2521 tcttttaaat acagaaccgg cactgggaag gtctaatggc tgtttttcct ttctcttttc
     2581 ttcaaaggag cgcatttttt cggctatgat gtttggatct tctggttgaa gttgaaagac
     2641 acttttaata tgaataaggt ttttttcttg aaagatactt ctgcggtagg caaaattatg
     2701 ctcgttagca ttgagatgct tgatgggatt taaagaattt aagctatcca gattgggctc
     2761 cagatagtca ctactaagaa ggacatcttt tatttcagag ccataagcac cagcattcat
     2821 aaaaactgcg ccaccaactg ttcctgggat tccagaagcg aattccaatc cggaaagtcc
     2881 ttcttcctta gcaaaatagc ataagtcttt taaagtggcg ccacagtatg cctctataaa
     2941 atttccttcg tgttttagtt gattcagtgc agaagtctga attacgatgc catctacacc
     3001 tttatcgctt atcaataaat tagaaccatt accgaggatg aagtaaggta tattaaaact
     3061 gagggcaact ttcaatatat caatcagttc ctctatattt tgcggttggc agaatacttc
     3121 cgctggacct ccaatcttaa aactggtata gggagctaaa ggcacatcaa aacggatatt
     3181 tcctttctct attagggtgg gcaattgttc ttttatcagt tgatctaaca tcattactta
     3241 ctcacattga aaaaacggat aacttcattg agcaattcca attcgctttt aatttccatg
     3301 cattttgctg gaagcacttc tttaaaatat tttccatagt atttatttga gacgcgtgaa
     3361 tccattatca gaacgatacc gcggtcttct ttactgcgaa taagtcttcc aaaaccctgt
     3421 cttaatttaa gcagagcatt tgggagcata tagtgcataa aagaatcttt attctcggta
     3481 ttaagtttgt caatcagagc ttccacaata ggttcagaag gcacttgaaa aggcaattta
     3541 aagaggataa gcatagaaag tgaatcgcct tgaatatcaa caccttccca gaaagaattg
     3601 gttcccaaga ggacggcatt tttatagctt ttaaattctt ccagcataga ggagcggcta
     3661 taagtttttc cctgagcaaa aagcggtctt ttgcgatgat acagaggctc acttagataa
     3721 tcataaacac tgtttaagtc cttgtaattc gtaaagagaa tcattgtgcc aacatcaacg
     3781 gaaagaacca gttgttcaac acatcctaag gcttgattga taaagaaacg gtccttgggc
     3841 tcagggagaa agctacttat catcaggagt gactgtttat cgtaatcaaa gggagaatct
     3901 acaatatatt cgtccagttg ttcaggtgga atgaggctta atccactttg agtaaaaaag
     3961 tatttaaaag attggcgtaa agcaagagta gcagaagtaa aaataatgct cggaatattc
     4021 ttataaagca tttcgtttaa ctggagagac acatcaacgg gtgcatagca aaaagtagag
     4081 acaggagttt ttgggtctgg acgcaagtca ttttctatcc agagtgcatt attcatcaaa
     4141 tctggatttt ccagaatatc cagaccaacc tggatatcta cacttctttt tactaaaccg
     4201 tttatggatt catttagggt atcgtaattg ggaacttgtt tgctgtttag catagaaaag
     4261 gtatcagcta aattggatag ttgtttcagg aaagagtgcc attgattatt cagcttatcc
     4321 agtgattcat ataaatgggg gaaatcttcg ggatttttaa ttctcagttt tgaataggaa
     4381 tctgcctgtt gacaaaggtc ttgcgcttca gtgaagagct ccagcgaaat cttacgcata
     4441 gtgctgattt gttcactcag actttttgta atcatctgga tttgttcatt gctggtttca
     4501 gggataacac ttcttttaag cataaaatca agctgattaa gaagacccac atttttcctt
     4561 tttgtagctg gagcgaactg attaaacaga tttactaaat cttgatatcc aacctcaaat
     4621 ccaagatttt tagacgcaga gtccattaaa ttatgagctt catctaccac cagataatca
     4681 tacttcccca gggtggtatt ttccattctt aaatcagtta gcagtaagga atgattagtg
     4741 actacgatag ttgaattttc tatatgtttt ctaagattga gcacatagca tttaccagag
     4801 tggggacatt ttcttcccat acattgataa cggtcagagc agattctttt ccagatgata
     4861 ttaaaacgtt ttcggtcaaa ggatgaattt tccgagatat ctccagattt tgtgagctgt
     4921 ttccaaatat aaagatagag caatgcctga gcttcataag gagttaaagc agtggattgt
     4981 tccatcaaca tatcattcca tcttcgttca catacataat tttctctacc tttaaccagt
     5041 gaggctttaa agggcagagg aagcatcttt ttcaattgag gcaggtcttt ataaaaaagc
     5101 tggtcttgca gatttttggt attagtagag accactaccc gagttttatt cttatgcgca
     5161 aaaatcatag ccggaacgag ataggcaaaa gattttccta ctccagttcc agcttcaatt
     5221 accagatgtt tagtattttg aaatgcttct ttcactttat ttgccatttc caattgacca
     5281 gaacggaatt cgtaatgagg aaacttctga gaaagcagac cttcggaatt gaaaacctca
     5341 tccagcgaaa tattatggaa gggaatatca ttttctatca catttggttt cgcatagtca
     5401 agaggagtag tgtgttctcc tttcagcaca tagcttcttt gatattccac cagtaaatgg
     5461 agataatcat acaaggaatt attcagttga gcctgtttac tgaggtccag caagcgagca
     5521 ttagtcatca ttgaatggtg cttgataatg tgttcggtca tagctaacaa aagtagacca
     5581 cttgcttcag catcggcagt agctctatga gcagcttccg gggtaatatg gaaaaacttt
     5641 agcatagtgc ttagtttatg gtcactgacg aaggggaaat agactcttga tatttcttgt
     5701 gtatcccaga atttattagt tagaacagca cctttattta aagcggaatg atagtttatg
     5761 aatccgatat caaaaggtac attatgtccc accagaacgg aatctccgac aaaagagaat
     5821 aaatcctgca gggcaatttt tgcttcagga gcggtggata aatcatcagg attaatatga
     5881 gtgaggaatt ctatatattt tggcattccc cgatgtggtt taaccagcgt ggagaattgg
     5941 tcttcaattt tccctgattt gaaacgggtg gcagctaatt ctataatttc atcacgggaa
     6001 gggtccagac ctgtggtctc tatatcaatg gctacaaaat ccagattatt ctttaaacca
     6061 gccagctctt ctttttccat tattgaggga tttcctattc gccctcttta tagagattat
     6121 agcgttttag tttctgaatc aagccttgac gtgagattcc cagttctttt gctgcttgag
     6181 tttgattcca gtcaaaagct tccattgcat cttgaatcat tttacgttcc agttcttcca
     6241 ttgcatcttt taatgttttc cgtccagaaa ggatactgat ggttttctga ttttcaatat
     6301 agcgtcgcac ttcatcagaa aaatcggtac ctctaataaa agaagcatct tcagccaaag
     6361 taacagctcg ttctatttca ttttccagct gtctaacatt tccgggccaa tcatattgtt
     6421 ccagcatttt cattgcttca tcagtgaatc cttgaacatt ttttcccatt ctggtgttat
     6481 atttatccag aaaatggatt gccagaagcg gaatatcacc agtccgattt tttaagggtg
     6541 gaacttctat gcggattaca ttcaatctgt aatacaggtc cagacggaaa gctccagatt
     6601 taaccctttc ttctaaagga acattagtgg cacaaacaac gcggacatca actttttctg
     6661 tctttgtgga accaactctt ttgatttcac cttcttgcag aaaacgaagc agcttagcct
     6721 gagtgctggg agttatatcc gctatttcat ccagaaagaa agtgccccca tccgctattt
     6781 caaaaaggcc tttcttgtca tatgcagctc cagtgaacga acctttaaca tgaccgaaaa
     6841 gttcgctttc cagaagagtt tcaggcaaag caccacaata ttgagctacg aacgctttat
     6901 tcttacgatt gcttctatag tgcaaagcac gagctaaaag ttctttacca gttccacttg
     6961 gtccttccag aaggacagtt gtaggtgtgt cttttacctt ttctattata tcaaaaatct
     7021 tgagcatctc tggactttgt ccgataatat cagagtatac atttcccata ttaagttgtt
     7081 ctctgatgct attatgcgtt ttttccagac cagcagaacg aatattttct atggtgataa
     7141 ttgcttgatt ggtgagtgca ctgagcagat ttatgatacg ttcagagaaa tagtgtgaac
     7201 cgctttgtgc aaaaagtaat accgttccca gaacattctt tcttatgaag agcggaagca
     7261 ctatgatatg attgtaagaa ggagcaaaat gcggttgctt gaagtctttt atgctattcg
     7321 tgtcatatgc catattacag agattcataa agatatcata atacttattc tctgaagaaa
     7381 aattctggaa tatcttatat tcccaggaat ctggaacggt ctcacttcta tgcagacaga
     7441 gtatgcctcc atctgcttct gatatatcaa ttagtccaga aagtgtttgt tctatcagca
     7501 catccagatc tgagatagtg tttagccttt gggttatttc atagaattgg ttaagcagat
     7561 tttcttcaaa cttaacatct cttaattgtg aagaatattc ccgggaaatc ttctgcatta
     7621 aaatatcatt cttctccaga agctttatag aaccatattt ctgtattatt ttcttattag
     7681 tctttaatat ttccagtgca gcttcccatt cttttaagtc aaaaagaacc tctgccagct
     7741 cataatttgc caaagaaagt tcataattat tggaggtctc cacaaaaagt tctatagcct
     7801 ggtgcagatg ttctttagcg gattccagat tttttcgttc cagtaatgct ctgagataat
     7861 gaattttccc taattcaaac aagcgatttc cttcacttgc catattcatc gcttcggtta
     7921 aataatattc tgcactatcg taatttctgg ttaaaatgaa gtagtaagct tgtttttgat
     7981 atccctctat gattttatct ctcgcattga tagatttgaa gaaatccaga ctttccacca
     8041 gataggtata tgcctcttta aatttatgca gtttagttag cacacttccc agattaccat
     8101 ataaatcagc cttaatgaaa tcgtcattaa cagaaggaag taattctaaa cctttaaaat
     8161 ataaatcata agccagagtc agttcatcct gtttctcata aacttcacct aagttattga
     8221 aagagcgaag taatccctca gtaaaattgt tctcctcact ttttttgatt gcctgttcat
     8281 aaagtgctat ggattgtgcc cagtcacctt ttttgaagta aagagcgccc agattgttta
     8341 atgatgctat aatattgcga taaaatccaa agtctattgc tgtctgtagt gaatttttta
     8401 aggcttcttc tgctttatca tattcattat ggtccatcca tgtgatgcca atattattat
     8461 atatatttgt gatattataa ggttcattaa gcattttttg tagtttaagt gcttcttcca
     8521 gatacatcaa tgatttttga ccgtcacctt tatcatccat caaaccaccg aggttattat
     8581 aacagataga gattccgttt agattgaagt gttccttttc caaagccaga cttttcagaa
     8641 aatattgttc acttctatcc caatcacctt gaaacatata aagtactgcc agattattat
     8701 atacggcagc caatccacta tggtcattgg caagactaaa gaatttttcc gcttctttaa
     8761 aattctcctc agattgttcc cattcattaa tatagtaaag gattttacct ttaatttttc
     8821 tggcttcacc ttgcagtaaa aggcgtttat ctttatcgtt gatagaggta ctttgatggc
     8881 tgattttttc cagttcttcc agtgcggaat cgtagtcttc tgtttcacca aggatatcag
     8941 ctttaagaag caatatcttc aatttccagt aagggtctat ggtctcgggg gaatatttat
     9001 tgattatttc caaagcaaag gcagtagaat ttacagcaaa aagattactg gcgagtaaat
     9061 agacaatttc ttctgctggt aagaagtttt ccgtgcagat gtgcaagctc tgacgaagat
     9121 attctatggc ttcatctttc ttgttggaat tggcataagc taagccaata tttttatgga
     9181 tgagcatttg tatgtcaggc tttttagtat gctgaagttg atattgataa agctgcagca
     9241 agctatcatt atcagataga ttttccagtt cttttatgac cgcttcaaaa gctgaagctt
     9301 catatttatt catctgcatc aaaatcttta ccttgatttg ttcttcactt ttatcttctg
     9361 ctaagttatc cagaagtttt tgaatcaagg gcgaagacag agcttccgga tgctgttcca
     9421 gccaaatcat cattgaaact tttttctgga ctacccaatc caaatcagga tgttctatta
     9481 atccagcgtt gtacaatttt tccaggtctt ttttaatgtt ttttttgcca ctaatatttt
     9541 tgatattatc ttcgttgaat ttttggtcta aaataaacat actgagtagc aattctttct
     9601 ggttttcagg tagattttcc aaagtctcaa aatatatctg taaagggtca taatttttat
     9661 ccagaaatgg agttaaatca aattcagttc cgttttcccc caattgcata tcattgaaaa
     9721 tcttttccag gataagcata ttaccttcaa ctatcttctg caaaatctca ctttctttac
     9781 cgacattttc tactgcttca ggaaaggcaa gttgtattat attttgaaca tcttccacag
     9841 ttaaaggcgg aactatctcc caatgggcaa aagggaaaag acgctgctga gaaagaaaaa
     9901 ctatttgaat accactgtct tcagcatatt gcaccagata ctgtaaataa tcccggctat
     9961 atttatctaa aatatcaccg tcatctatga ttattgcacg aggagtcaat aagttatgtc
    10021 tattcaactg ctctgtgaga tagtagaaaa aatcatattt tctgcccgtg cgatattgtt
    10081 gattgtattc ctgataaaat tcttccaagg cgtcaggttc aacatcactt attatgggta
    10141 gtagcagaga aaattgattg agataaagag ggtgcggcga gtagaaatat actttttcgt
    10201 aagtattttg aagagtttcc atcagaggtt ttatcaggta tgactttcct gagccagctt
    10261 ttcccgttat ctctatcaaa tggctatgtg acttgtccag taaaaggttc aagacctttg
    10321 acagcgaaaa gcgctgactt atataattat gtagcttttg tgttaaacct tccgtcatat
    10381 tttttacctc tgttttaaaa agaagcattt acatttgagt tgtaaattat atttacacta
    10441 tactgtcaag aaaaataatc agctattttc aa
//
LOCUS       DAORZF010000051        10090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_5007_length_10090_cov_357.679322, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000051 DAORZF010000000
VERSION     DAORZF010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 10090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10090
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5007_length_10090_cov_357.679322"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..446
                     /locus_tag="PK015_06180"
     CDS             15..446
                     /locus_tag="PK015_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34091.1"
                     /translation="MKKIIFLLVFISLVGYLIGQYVPVEIVNNAEDYLGRQLVTRFRD
                     EIRKSPSYTVTYSSDEPHFKVKIDTMDRYKGDSLYEGISTIYNYTILIAVNGIDIYCY
                     SQLGYAGKDVLNEVAFQIYSDLDEFIETFKSYLIKLLEEYE"
     gene            complement(823..2511)
                     /locus_tag="PK015_06185"
     CDS             complement(823..2511)
                     /locus_tag="PK015_06185"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOL34092.1"
                     /translation="MLKKIIFRPFTFVVILMVLIEAMISVLPQQVFGMLIDTISSNTT
                     ELTKYIIYPLVRIIAQLSDLPLMTQLFILYLLLSLFSLFVSILRGYCVTYNGERILFN
                     IRKKLFSNLIRSNYTYLNTLTSGETALNIVSDVENLRNLIIGPVNGLLIDLISVFLIG
                     FICFRISAELSILMLIPIPIVIISSFIIGNKQLKISTHLREHLSNLTSTTINRIKGFL
                     LVKLFAQENKEEEQYSTLLSKYFHQAVKSLKTTLALFPLTSGVQIIVTVGVLYVGILR
                     VQQQVLSVGELLIFIQYLGRFYIPFINIARFYNSIARSIVSYKRLENTFLETERNRDI
                     IVNGIIPTANYAGLETQTAIEFDDVTFSYCTTDYSPNVIESLSFKVMRGEKVSIVGES
                     GKGKTTVLNLIVRLISPQKGKIKFNGIDINEYDTNYLRSKIGYLTQNNIMYNIPLIDN
                     VRYGVPDKSIEDVVRALSQVNMQRYTSSNYLFKTMGEGGELLSGGEKQRVAIARLVLC
                     NPDVILLDEPIANLDIDNAKCVMDLIFNLFSDKTIIITSHQPLAIAYSTRSILL"
     assembly_gap    2746..2845
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2846..3174)
                     /locus_tag="PK015_06190"
     CDS             complement(<2846..3174)
                     /locus_tag="PK015_06190"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOL34093.1"
                     /translation="MIHPDFVKIHKSAPEIGIKVNLYTNVYFLSDDIVDMFKKYTPSM
                     LDITLYGFDKESYKAFTKISDSFDNASKNLQKYSDSGIPITTRTQLHRLNYHDIDKYK
                     LLAASFGL"
     gene            complement(3734..5275)
                     /locus_tag="PK015_06195"
     CDS             complement(3734..5275)
                     /locus_tag="PK015_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOL34094.1"
                     /translation="MVGIVRTYTTLGIDGFQLTVECDASGESFGVNIVGMASNAVKES
                     KDRIFAAVNNSGFKTPVRRFTINLAPADIKKDSAALDLPIAIAILQSIKQIPEEYLTK
                     TALIGELSLDGNLRPVNGVLPIALAAKKDGMDALVLPYENADEAAIIDGLNVIPVTSL
                     RETVQYLRGEIDIAPASVDRDKIFSVLNDFKIDMSDVKGQYQVKRALEVAAAGGHNLL
                     MIGPPGSGKTMLARRVPTILPELSLDEALEATKIHSVAGYSKYFRNGILTTRPFRAPH
                     HTISDIALIGGGAFPRPGEVSLAHRGVLFLDELPEFKRGVLEVLRQPLEDGIVTISRA
                     ASSLTFPAEFMLIASMNPCPCGYFGANIPNHICNCDFGAIQRYRSKISGPLLDRIDIH
                     VEVPTVSYAELSSLPSGDTSTEIRNRVNKARDIQRQRYAQEGIFCNAQMSSKLLRKYC
                     KLDLQSQALMQNAIDHLGYSARVFDRILKVARTIADLEEQENITSDHISEAIQYRTLD
                     RKYWS"
     gene            complement(5286..6884)
                     /locus_tag="PK015_06200"
     CDS             complement(5286..6884)
                     /locus_tag="PK015_06200"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOL34095.1"
                     /translation="MSYILFTGLLYAEIVINEVCYDPKGADSGYEWIELYNNGDSDIN
                     LQGAKILCGGRTFQQVYELPSFILRAHRFLLIGESQVLQAQLITTLGFQNGGSETDGI
                     RYVSPDGTYTDTLLYDSPNTNNLPDDTGAPGTSFAPDVPEGYSLARIIDGLDTNDCEL
                     DFISESQPTPGLPNHRYIDYALISPEVFLSDSFWHYQVAVKNLSSFTTNVSANLNIFL
                     DESSIAEYDVPIIAPGDSLLYAGLIPVEDESNHMVKFILHLIDDPDLTNNQCSFWLWE
                     SEPQPPVINEIMYNPLTGLQEWVEIYAIDSAPGDYILKDQAENQCLFSLPSSSGYFIL
                     CPNSDSFISQYPSCPSSAVLQSNGWTSLNNDGDSLYLFDEEQNLLDQMSYIGDNTHKG
                     ISLERYLTATQETKWRYCLNETGSTPGLPNSQNPPFPQFTGTLHISGSPCNPQKGENI
                     NIYYKLNSTQSYVVCYVYDRQGRKVRVLADNLKVSSEGNLSWDGKDASGKYLPRGLYI
                     ISWKSKAQDGGKILNRQFSVVLYH"
     gene            complement(6950..9451)
                     /locus_tag="PK015_06205"
     CDS             complement(6950..9451)
                     /locus_tag="PK015_06205"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HOL34096.1"
                     /translation="MKEGFLTRNDFHSIFFSDLAERGNEDIKTLFLRHLEYSMVKDIT
                     NVQPWDIYFALSLSLRDKLIQRWLRTQYEYRKQDVKKVYYLSMEYLIGRLLVNTLINL
                     GEYELSYDMLKEFGYKLEDIAELEPDMGLGNGGLGRLAACFMDSLATQAYPAYGYGIR
                     YEFGIFKQSIIQGYQVEEADRWLKKGCPWEIQRPELTYRVRFGGEVVTETLPDGKILY
                     YWLNTDDVMAVAWDIPIPGYKVDNVNNLRLWQATATDEFDLEYFNNGDYVRAVEKKNI
                     SENISKVLYPNDNIHLGKVLRLQQEYFFVSATLQDIFAAWKQDHDNFTDLPEKVAIQL
                     NDTHPALVIPEMMRILIDEEQLDFETAWDITRQCCSYTNHTILPEALEKWSVKLFEEL
                     LPRHLMLIYQINEHIMNQVIQLYPGNIDKMRNLSIIEEGPEKKIRMAQLCLHGTHTVN
                     GVAELHTRILKERIFPDFYEMYPERFQNKTNGITPRLWLRTCNPQLASLITEHIGESW
                     VANLDYIRGIEQYAKDQEFLDEFLEIKEINKRELTRYIFKTTGIRVRQDSIFSSQIKR
                     IHEYKRQLLNALGAIALYFRIKDNPELEFTPRTFIFAGKAAPGYYLAKLIIKFINNIG
                     IVINKDPVVADRLKIVFLPNYSVSLAEKIIPASDVSVQISTAGYEASGTGNMKFALNG
                     ALTLGTLDGANVEMAEEIGMENMFIFGMNAQEVTELKQRGYNPREYYNNDPELRRVVD
                     AIATDMFSPKEKGIFLPIVSSLLDMGDVYCIMADFKPFLDAQLRIDQLWQDRYAWAEK
                     AIINIARMGKFSSDRAIKEYANDIWHIKPVQLDLP"
     gene            9800..9928
                     /locus_tag="PK015_06210"
     CDS             9800..9928
                     /locus_tag="PK015_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34097.1"
                     /translation="MTKKQESQGASNAGRDGLIRQPDPTTKGRWYGTANGSSGREK"
BASE COUNT         3208 a         1818 c         1814 g         3150 t
ORIGIN      
        1 cagtaggagg aaagatgaag aagataatat ttttgttagt ttttataagt ctggtaggct
       61 atcttattgg tcaatatgta ccggtagaga ttgttaataa tgcggaagat tatcttggca
      121 ggcagctggt cacaaggttc cgggatgaga tacgtaaatc accttcatat accgtaactt
      181 attcttctga tgaacctcat ttcaaagtta agattgatac catggatagg tataaaggtg
      241 atagcctcta tgagggtatc tctactatct acaattatac tattttaata gctgtaaatg
      301 ggatagatat atattgttac tctcaattag gttatgcagg aaaagatgtg cttaatgaag
      361 tagcattcca gatatattca gatttggatg aatttataga aaccttcaag tcatatctga
      421 ttaagctgtt ggaagaatat gagtagaggt tttactcata atccttatct gataaaacaa
      481 agtggacgga taaaaatagc tattcaaagt ttagtcgtaa taagcagtta agaaaaaacg
      541 agttgcataa ctattattaa ataaattacc tgtctgagaa agagatttac aataaatctg
      601 gttataataa tataattgtt tccaaaaacc ttaaataaaa gctaaaggta aaaactgtaa
      661 catattccta aataaattac aaaataatga gttgaattgt ataccactcc aaaattgaga
      721 gttaaaacaa gaatttatat cagtcttcat attaaaactc attgtatgaa tataacgaat
      781 ccacacagtt taccaaatga ttctcaacaa atacaataga cattaaagca atatggatct
      841 tgttgaataa gctatcgcca atggttgatg tgaagtaata ataatcgtct tatcgctaaa
      901 caggttaaat attaaatcca taacgcattt ggcattatca atatcaagat tagctattgg
      961 ctcatcaaga agaatgacat ctggattaca taatactaat cttgctatcg ctactctttg
     1021 tttttcacct ccagataaca attcccctcc ctctcccata gttttgaaca agtaatttga
     1081 acttgtgtag cgctgcatat ttacttgaga caatgctctg actacatcct caatggactt
     1141 gtctgggacc ccataacgaa cattatctat caagggtata ttatacataa tattgttctg
     1201 agtcaagtag ccaatcttag acctaaggta atttgtgtca tattcgttta tgtctattcc
     1261 gttaaatttt atttttccct tctgaggaga tattaatctc acaattaggt ttaacacagt
     1321 tgttttacct ttaccgcttt cacctacaat agaaaccttc tctcctcgca tcaccttaaa
     1381 gctcaatgat tctattacat ttggactata atcggttgta caataactga aagtcacatc
     1441 atcaaattct atagccgttt gtgtctccaa accagcataa tttgctgtag ggataattcc
     1501 attaacaatg atatctctat tccgctcagt ttccagaaat gtgttctcta atcttttata
     1561 tgatactata gaccttgcaa tactgttata gaaccttgca atattaataa atggtatata
     1621 aaaacgacct aaatattgaa taaaaatcaa taattctcca acgctaagaa cctgctgttg
     1681 aactctcaag atacctacat atagtactcc taccgtgaca ataatctgga ctccactggt
     1741 tagcgggaaa agtgcgagtg ttgttttcaa actttttact gcttggtgga aatacttgga
     1801 gagcaaagta gaatattgct cctcttcttt attctcttga gcaaatagtt ttacaagcaa
     1861 gaaaccttta attctattaa ttgttgtaga tgtcagattt gacaagtgtt ctcgtaaatg
     1921 cgtggatatc ttaagttgct tatttccaat gatgaaacta ctaataataa ctattggtat
     1981 cgggattagc attagtattg atagctctgc tgatattcta aagcatataa acccgatcag
     2041 aaaaacactg atcagatcaa tgagcaaacc attaacaggt ccaattatta aattcctcag
     2101 attttctaca tccgacacta tgtttaaggc agtttcacca gatgtgagag tgttaagata
     2161 tgtataatta cttcttatta ggttagaaaa tagcttcttt ctaatattaa ataaaattct
     2221 ctctccattg taagtcacgc aatatcccct tagaattgaa acaaataacg agaacaaaga
     2281 taacaataga tacaatataa ataattgtgt cattagtgga agatctgaga gttgtgcaat
     2341 gatccttact aatgggtaaa tgatgtattt tgtcagttca gtcgtattag atgaaatagt
     2401 atcaattagc attccaaaaa cttgttgggg tagcactgag atcatcgctt caattagcac
     2461 catcaatata acaacaaaag taaacgggcg aaaaatgatt ttttttaaca taactaatct
     2521 atactattta ttttttgagg ggtgatataa ttggggtaat taattcgcag taataggaat
     2581 tatcatcatc tttaaatgat ttgccacaac cgccagccat aacaattttt actaatttga
     2641 ctgatggact ttcgtaagta tccatgtaac ctcctattta ttttagcctt gcaatatcta
     2701 atctttgcga tattattcat caggaaaatc cttcataaat ggttcnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnagtcc aaaggatgcg gcaagtagtt tatatttatc
     2881 aatatcgtga tagtttaatc tatgaagctg ggtcctggtt gtaattggta ttcccgaatc
     2941 actatacttt tgtaagtttt ttgaagcatt atcaaaggag tcagatattt tagtgaaagc
     3001 tttataagat tctttatcaa atccgtagag agtaatgtcc agcattgacg gagtatactt
     3061 tttaaacata tcaacaatat cgtcactaag gaaatacaca tttgtgtaga gattgacctt
     3121 tattccaatc tctggcgcag acttgtgtat tttaacaaag tctggatgaa tcaacggctc
     3181 tccctcagtt aatagtatac tgtagcatcc acattcgtta agctcatcaa gaacccatac
     3241 tacacgagca taatcttgat aatttatgtt gttttatcca actaacagtg taaccagtca
     3301 aaacagcatc cgttagtcaa ttcaagtaca gttgataaca tattgcgatt tatcctattg
     3361 cgtaatatta cctgctcctc tctattgttg atatttgata acttgtttga atccccctta
     3421 ttaatcggtt tgattgtttc agaaaaactt tataatcaga ttcaatcata tccacttgta
     3481 ccttatattt tccgcaattt tgaggaatag cgtctgcatt ggcattccat ccttctacca
     3541 ccgattcttt ttactcttag gttttcaagt ttctctaatt tcatacactt ctatccaaat
     3601 tggctttgct ctacataagt gttggaacga aattcaactt gctgttattc attgttagta
     3661 tggtaaaaag ttatttctat ttgtcaagga gaattatctt tattagggaa ttaaattgta
     3721 taattttatt gaatcaactc cagtattttc tatccagagt gcggtattgg atagcttcag
     3781 agatatggtc gctggtgata ttttcttgtt cttccaggtc agctatggta cgagcaactt
     3841 ttaggatgcg gtcaaaaacg cgagcggaat aacctaaatg gtcaatagca ttttgcataa
     3901 gagcctggct ttgaaggtca agtttacagt atttacgaag taatttagag ctcatttgag
     3961 cattgcagaa gattccttct tgagcatagc gttggcgttg gatatcccga gctttattaa
     4021 ccctatttcg gatttctgta gaagtatcac cagatgggag cgaagaaagc tcagcgtagc
     4081 taaccgttgg gacttccaca tgaatgtcaa tacggtcaag aagaggaccg gagattttac
     4141 tgcgatacct ttgaatggcg ccaaaatcac agttgcaaat atgattggga atattagcgc
     4201 caaaatatcc acagggacag ggattcatag aagctatgag cataaattca gctgggaaag
     4261 tgagacttga agcagcacgg gaaatagtaa cgattccatc ttccagaggt tgacgaagca
     4321 cttccagaac acctcttttg aattctggta attcatccag aaacaggaca cctctatgag
     4381 ccagagaaac ttcacctgga cgagggaaag cacctcctcc aatcaatgct atatcactga
     4441 tagtatgatg tggtgcccga aaaggacggg tggttagtat accattgcgg aaatatttag
     4501 aatagccggc aacggaatgg attttagtag cttccagagc ttcatctaaa gaaagttcag
     4561 gaagaatagt aggaacacga cgagccagca tagttttccc gcttccaggg ggtccaatca
     4621 tcaatagatt atgacctcca gcagcagcaa cttccagagc tcttttcacc tgatattgac
     4681 ctttaacatc gctcatatct atcttaaaat cattcagaac agagaagatt ttatctctat
     4741 ccacggaagc gggagcaata tctatctctc cacgcagata ttgaactgtt tctctgaggg
     4801 aagtgacagg aataacattt aatccatcta tgatggcagc ttcatcggca ttttcataag
     4861 gcagtaccag ggcgtccatt ccatcttttt ttgccgctaa agctattggc agcactccat
     4921 taactggtct taagttacca tccagggaaa gttctccaat taaagcggtt ttagttagat
     4981 actcttctgg aatctgtttt atgctttgca ggatggcaat agcaatgggc aaatccagag
     5041 cagcgctatc ctttttaata tcagcaggtg caagattgat ggtgaaacgt cttacaggag
     5101 ttttaaatcc agaattatta acagcagcaa aaatacggtc tttactttcc ttaacagcgt
     5161 tggatgccat ccctactata tttacaccaa agctttctcc tgaggcatca cattcaactg
     5221 taagttgaaa tccatcaata ccaagtgtgg tgtaagttct tactattcca accataaatc
     5281 acctcttaat gatataatac gactgagaat tgacgattta aaatctttcc tccatcctga
     5341 gccttagatt tccaggagat gatgtaaaga ccgcgaggaa gatattttcc cgaagcatct
     5401 tttccatccc agcttaaatt tccctcggac gaaaccttta aattatcagc taaaacacgg
     5461 acttttcttc cttgacggtc gtaaacatag caaaccacat agctttgagt ggaattaagc
     5521 ttgtaataaa tatttatgtt ttcacccttt tgaggattgc aaggactgcc agaaatatgt
     5581 aaggtaccag taaattgagg aaagggagga ttctgactat taggtaatcc cggagtggaa
     5641 cctgtttcat tcagacaata acgccattta gtttcctgag tagccgttaa atagcgttcc
     5701 agggagattc ctttatgagt attatctcca atataggaca tctggtcaag cagattctgt
     5761 tcttcatcaa agagatagag agaatcccca tcattattaa gagaggtcca tccatttgat
     5821 tggagcacag ctgaagaagg acaagaagga tattgagaga taaagctatc agaattggga
     5881 cagaggataa aataacctga agaggaagga agggagaata aacattgatt ttcggcctgg
     5941 tctttcagaa tgtaatcacc gggagcagaa tcaattgcat agatttccac ccattcctgt
     6001 aaaccagtta gcggattata cataatttca ttgataaccg gaggttgagg ctcactttcc
     6061 catagccaga aggagcattg attattggtt aaatcagggt catcaataag atgtaagata
     6121 aattttacca tatggttaga ttcatcttcc acaggtatta aacctgcata taataaggaa
     6181 tcaccaggtg caattatagg aacatcatat tcagcaatag aactctcatc cagaaagata
     6241 tttaggttag cactgacatt ggtggtaaag gaagaaagat ttttaactgc tacttgataa
     6301 tgccagaaag aatctgataa aaagacttca ggtgagatta gggcataatc aatataacga
     6361 tgattgggca gacctggagt gggttggctt tcagagataa aatcaagttc gcaatcattt
     6421 gtgtccagac cgtcaattat ccttgccagt gaatatcctt cagggacatc aggagcaaaa
     6481 gaagttcctg gagcaccagt atcatcaggt aaattattgg tgttgggaga atcataaagc
     6541 agagtatcag tataagttcc atcgggactt acataacgaa taccgtccgt ttcagaccct
     6601 ccattttgaa atcccagagt agtaattaac tgtgcttgaa gaacctgaga ttcacctatg
     6661 agaagaaaac gatgtgcacg caggataaag gaaggcaatt catagacctg ttgaaaagtt
     6721 cttccaccac atagtatttt tgcaccttgc aagtttatat cactatcacc attattatag
     6781 agttctatcc attcataacc agaatctgca cctttggggt cataacatac ttcattgatg
     6841 acgatttcag catagagcag accagtaaat aaaatatagc tcaatcctgt aatcagaaga
     6901 aactgtaacc acctgatata acgcatttta aaagattaaa cttaacaaat cagggaaggt
     6961 ccagctgtac cggtttgata tgccagatat cgttagcata ttctttaatc gcgcggtcgg
     7021 aagaaaattt tcccatgcga gcaatattga ttatagcttt ttctgcccag gcataacgat
     7081 cctgccatag ctgatcaatt cttaactgag catctaaaaa aggtttaaaa tctgccatta
     7141 tacaataaac atctcccata tccagaagag agctcactat aggtaaaaat attccttttt
     7201 ctttgggaga aaacatatca gtggcaatag catctacaac tcttctcagt tcagggtcat
     7261 tattataata ttcacgagga ttatagcctc tctgtttcaa ttctgtaact tcctgggcat
     7321 tcataccaaa gatgaacata ttttccattc caatctcttc tgccatttct acatttgctc
     7381 catctaaagt tcccaaagta agtgctccat tgagagcaaa tttcatattt cctgtcccac
     7441 tggcttcata tccagcagtg gaaatctgaa ctgaaacatc ggaagcgggt ataatttttt
     7501 cagctaaaga tacggaataa ttgggtaaga aaacgatttt caaacggtct gctactacag
     7561 ggtctttatt gatgactatt cctatattat tgataaattt gatgataagt tttgccagat
     7621 aataacctgg agcggctttt ccagcaaaga taaaggtgcg cggagtaaat tcaagttcgg
     7681 gattatcttt tatgcggaaa taaagtgcta ttgcacctaa tgcattgagt agctgtcgct
     7741 tgtattcatg gattcgtttt atctgagaag aaaaaatact atcctgacgc actcgtatgc
     7801 cagtagtttt aaaaatataa cgagtgagtt ctcttttatt tatttccttg atttctaaga
     7861 attcatctaa aaattcctgg tctttagcat attgttcaat accgcggatg taatccaagt
     7921 ttgcaaccca gctttcacca atatgttcag tgattaaact ggcaagttga gggttacagg
     7981 tgcgaagcca taaacgggga gtgatgccat tggtcttatt ttgaaagcgt tcggggtaca
     8041 tttcatagaa atcggggaaa atgcgttctt tcagaatacg agtatgcaat tctgccacac
     8101 catttacggt atgagtgcca tgtaaacaga gttgtgccat tctaattttc ttttctggac
     8161 cttcttcaat gatgcttaaa tttcgcattt tatcaatatt accaggataa agctgaataa
     8221 cttgattcat aatatgctca tttatctgat agataagcat cagatgacgg ggcagcaact
     8281 cttcaaaaag ttttacactc catttttcta aagcttcagg aagaatagtg tgattagtat
     8341 agctacagca ttgacgagtt atatcccagg cggtttcaaa atccagttgt tcttcatcta
     8401 tcaggatacg catcatttcg gggattacta aagcaggatg agtatcattt aactgaatag
     8461 ccaccttttc aggtaaatca gtaaagttat cgtggtcttg tttccatgca gcaaaaatat
     8521 cctgcagggt agcacttaca aagaagtatt cttgctgtag acgcaggact tttccgagat
     8581 gaatattatc gttagggtag aggaccttag atatattttc ggagatattt ttcttttcta
     8641 ccgctcggac ataatcacca ttattaaaat attccaggtc aaattcgtcc gtggcagtag
     8701 cttgccagag tcttaaattg ttaacattat caaccttata gccagggata ggaatgtccc
     8761 aggcaactgc cataacatca tcagtattta accaatagta taaaattttg ccatcaggaa
     8821 gagtttctgt gacaacttct ccgccaaatc gtactctata ggttagttct ggtctctgaa
     8881 tttcccaggg acatcctttc tttaaccagc ggtctgcctc ttcaacttga taaccttgaa
     8941 tgatactttg tttgaaaatg ccaaattcgt aacggatacc atagccataa gcaggatagg
     9001 cttgtgtggc aagagaatcc ataaaacagg cagctaatct acctaaacct ccattaccta
     9061 agcccatatc tggttctaat tcagctatat cttccagttt ataaccaaat tctttaagca
     9121 tatcataact aagttcatat tcaccgagat tgataagggt attgactaat aatcttccaa
     9181 taagatattc catagagaga taatatactt tcttaacatc ctgttttcgg tattcgtatt
     9241 gagttcttaa ccagcgctgg attaatttgt ctcttaaact aagactcagg gcaaaataaa
     9301 tgtcccaggg ttggacattg gtgatatcct tgaccataga atattctaaa tgcctgagaa
     9361 ataatgtctt tatatcttca tttcctctct ctgctaaatc agagaagaaa attgaatgaa
     9421 aatcattacg ggtcaggaaa ccttctttca ttaatgcctc ttctttcaat tataacttgt
     9481 ttgacagtca tcaaatagta gttctgatgt caagggaaaa ttgactaagt gtggttttaa
     9541 atattgtgct aatattatag agtattattt aattctgcaa atcgtctgaa acggtggaag
     9601 gcaagtatca ccatttctgc aaataaattt ttgaatatgc tttcaatata ttacttatgc
     9661 ttacaagttc ccaatttgta attatccgat attttttaca tttcttatca tattgtttgg
     9721 aaaggggttg accaaggttg cgacgggttc tatttagaat ccataaatca tcagttaggt
     9781 aaccgagaag aagatcaaaa tgacaaaaaa acaagaatcg caaggagcaa gcaatgctgg
     9841 aagagatggt ttaatcaggc agccagatcc tactactaag ggaagatggt atggaacagc
     9901 caatggaagc tcaggcagag aaaaataaag tattaaagtt aggatagacc atttaggtga
     9961 tttagatgga caagaccggt ttagttgatg ttgaaagctc tataattgat gattcattcg
    10021 cagatgactt ccataaatgt ctctgcctac aaatgaattc ggattaaagt tatttataga
    10081 gatagaggaa
//
LOCUS       DAORZF010000052         9900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_5128_length_9900_cov_350.736821, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000052 DAORZF010000000
VERSION     DAORZF010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 9900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9900
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5128_length_9900_cov_350.736821"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2703
                     /locus_tag="PK015_06215"
     CDS             <1..2703
                     /locus_tag="PK015_06215"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOL34098.1"
                     /translation="FSTLADTKIEETPNYEQYLNDKIAYQNRDNYLTITQYYQFMEET
                     SNNYANICRLITAGTSVQGRPIYFLKITDNPDIEEAEPEFNYISTIHGDEIVGYDLCI
                     RLIQLLTSEYETNPRIANIVNTTEIWICPLLNPDGYVLHRRNNASNIDLNRNFPLPNG
                     ELHPDSNPWAPETIAIMNLFNEHNFVLGGEFHGGECIVYCPWSYTRSSTGENELLYHI
                     ASDYSIHNPYLGNSFFYPNNIFCGSTSYPLLGTLNDWGYAFTSSTQITFEIYEIKSPP
                     ASELPGLWNDNKEAMLYFLESVHRGVYGLVTSNTGRPLKASISVQDCNKIVYSDPEVG
                     DYYRLLLPGTYNITAYAEGYEPQTVQVTVPLSGSIEYNFALNPAVTVDLKGQFRDFEG
                     NLIPYTTVNLNTIPATNTNFNSKGFFTFNDLMEGYYNIHFSNNNEDIYETNFLLTKEK
                     NEAVFTPVESSLIFYDPCEDLSNWSTIGPWGVTNYQGETVITDSPSGNYAPGGTYTLT
                     LANPISLEFIKKPVLMIKARFILIRKWDNVYIQGSTHAENWIDLGILTGYGYAWEEIY
                     YPLNQFSGHNFYLRFVLQSRNNYGYDGIYIDEIKITGIDYSQVIYGDVDSDDLITMND
                     VQVLLQYLVGFDPVPLIDPLPWSEYRIHSADVDDNEVLVSVDAYLISRYIQNPKYRYE
                     VQNGEELELPQMNISFELIENPAETEVLFHLNPPNALNCLNWELTQADSIQNLGIGLI
                     PEQTFLYAFNPEQAKFALINPEKAPLESFSLHFNTNLANIDFLYNLNGQIGVIHLPTG
                     TNLNDTLIPEIPFALNQNYPNPFNSFTCIKFSLPEEKKTFLGVYNIRGQLVKTLINEV
                     KSSGEYDILWDGCDNYGQSVSSGVYFCILKSGDRLQTRKIILIK"
     gene            2881..4122
                     /locus_tag="PK015_06220"
     CDS             2881..4122
                     /locus_tag="PK015_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34099.1"
                     /translation="MYNVKWLYCFIFTLVCFIIFLGLFSCSSEVSKPRTLEELEKPFG
                     IPPSEKPANPKSFNIIVDASASMYGFTGENSEYLNLLNTIIAKIPQDAKINFYSFGQG
                     SLKMEGDLRANLYKLSRRDFYKEPRTDLTKPFNLIREDKNSVNLILTDGVQSTQHSQQ
                     DYVIFAKELNSYLEGNGFFALMGKLVSFEGSYFSEYARKYIDIEAGGKRPIYCLAFGN
                     RKYADFVQKKIRDLFENCFDFGVILPNHLKYTQDESGKNEPEKLVHISNDEKLPVAYF
                     TLDKGHFQYLKFNLKGYEDRFGTTIDYAIAYQAKNNPNFVELQDKRGNIKANKVAGTD
                     KITFKIPFEEDNPGTYRILLSFRKTLPSWIKEWSTDDDSKPEFQTKTYHLANWMQFIL
                     DNFEDYKFLTTTRYYLQIERK"
     gene            4123..>4488
                     /locus_tag="PK015_06225"
     CDS             4123..>4488
                     /locus_tag="PK015_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34100.1"
                     /translation="MLPNISFFWIFRPKIYIENWHNEMNQSVFIGLMVNIIFIAIYAA
                     LRYRREKESQRFWMAFLFSMVINLIYIIYTAIKIDSEMVTDSYRFLYILNFMLVMVLC
                     DFLLFYLVSFFLRAKKYFCR"
     assembly_gap    4489..4588
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4589..6057
                     /locus_tag="PK015_06230"
     CDS             <4589..6057
                     /locus_tag="PK015_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34101.1"
                     /translation="TVPYYVFSVGGTGARCLESLIYLCAMGIHLDQPLFPIFVDPDQS
                     NGNITRTIKLIQKYKLIREHLPRPSKDYLFNTEIIYSDKENSPNTTVKIPNLYNPNSG
                     LDPAQNKLSHFIEYETKLQGTDYKFLADLLFSKEELNMDMSAGYRGIPSIGSILMTDI
                     QNQAFWTELRGRLEEDTASRVFIFASLFGGTGASGYPVISKLIKNASPTTKVGGVLML
                     PYFKLKDPENLQKYQESLKKEKIMPNSKSFMINAKTACEFYKNNYSQIDSNYFLGDDF
                     EHCKQYENYAIGSAEQKNDAHMLELMAAYAAIDFFHRESGNFKPFYVIQVKNPDKNAD
                     ITDVVAEDIPHQDKEKPFERFALLYHYINELFELIDKDNAKLLDKIAWLRDLKIDDKK
                     VRARDIIDKKTELSSLKELMDSFQEWIYQNHNNYAKLNILDYELKLDNLLTNNPNKYK
                     GYHISHLDTKFYGKKTNQNDLLGDMLEAMSLAKITEGR"
     gene            6061..9339
                     /locus_tag="PK015_06235"
     CDS             6061..9339
                     /locus_tag="PK015_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34102.1"
                     /translation="MPRYLLPKHKSLPQTTPGQWKELTITDFTTFVNEMEVSPDSKIN
                     VTSIPSPWARMLLFKEAIKSTNHLIHKEVMSNILDVLEIIFYYELMNINIEYKEIKIK
                     NDEKNKFLKILYDLSPEEIKKDDIVSIGLLIASRDSNDKFVLAGTSPFSLVFTPMNLK
                     HKEGNTVFPRYFRERPVPLKERPKDFQKWINSNFLTKLRKDGNYPDLVKALSDEEGIC
                     AESLKESLTQEILLPCDFLLESSLGNLLEKINTKKISSSYLLKPRKENEIPPLVIDPS
                     KSLYASEYYNGYNYLQDFKYEELKNSNRDFLPGENIKYPWILPQEDFFQPVLIKYKYR
                     LNNNRLILGSYKDSSDMKYALPLTDTFFNYFNYDDVQDMLSIQEISDKTVRVTLKIPI
                     QNGDYIEIKRDYFQDLTNSKENRIINYDDRDIDTPLPHIMIWPPLHPDNWKDSYYAFV
                     YGKRYKDGINQEEMVPLEFKDQDFKDINYERSRKADKIEIFRLETLPTYIVISDIASN
                     GKGLLILNHKSLTTIMNPEKSAKVGIDFGTSHTNIAITIDNSEPEVLKYSSGFLGKNL
                     NDNDFITLIRFSEPELGREQIPNLIYNYLKQYFLPNSLSEIHNNQSVDMPLPSMILME
                     GDTDSYEALLNTSIAFSKDDVGLFNYDLDGMTIKKTYVQQTNLKWEPQLIKQQASKEY
                     LRILLLLLKYELIKRGVDLKKVEYHWAFPKSFSETLISDYKKMWRELVGDVYKITDES
                     KAALLYFDHKGILPRNTPDIAIIIDTGGGSSDISIWQDGQIHMLYSSLWAGKNLVGFE
                     KDNKIYSIIYDAIINLPSTKRDIFQNIKGFQNQLNYILYSLDETTLSNLARSDSFYKI
                     RFIILYFWSALLYEIGIQCRAIDMDNIKKINLFLAGNGSRFACWSSGAVDKMDEQEQE
                     IYQKIIKETIPFKNEVEIHTSAAQDKKREVAIGLCRGNEELRAKEASKRQLIAEKVFI
                     EANSDLSNMTIDEFNELILQNGHNIHLDQNNSEIVKFHNTFFEVLAKSNLYRDRLKND
                     RALSNLESIKNILIGDWGKFVGELRGVIEDNIKNYNSISSSVFTLEMQITINRLHRYL
                     SENQ"
     gene            9359..>9900
                     /locus_tag="PK015_06240"
     CDS             9359..>9900
                     /locus_tag="PK015_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34103.1"
                     /translation="MSRLILGLVILEVAVLALLIIIICKKLEKMETAKEPHRHSRDDA
                     QIQNLYTILNSHNTKLAKLEEMEERIKQLENRLEQLDQIKKPEPENTVNPPIEILPPK
                     AKEETNNDNKIWVWRSEEGLKKLEPVSNPKGLYLIKDGAKYLLYLDNFSKEKINDIMR
                     LYREIIDFPANIQINDQIVM"
BASE COUNT         3417 a         1573 c         1675 g         3135 t
ORIGIN      
        1 ttttcaactc ttgctgatac taaaatagaa gaaactccca actacgaaca atatttaaat
       61 gacaaaattg cttatcaaaa tagagataac tatttaacca tcacacaata ctatcaattt
      121 atggaggaaa cctctaataa ttatgctaat atttgtcgtt tgattacagc aggaacatcc
      181 gttcagggaa gacctattta ctttttaaaa ataactgata atccagatat agaagaagct
      241 gaacccgagt ttaactatat ctctaccatt catggagatg aaattgtagg ttatgattta
      301 tgcatcagat taatccagtt attaacttct gaatatgaga ccaatccccg tattgccaat
      361 atagtgaaca ccaccgaaat ctggatttgt cctctgctta atcctgatgg ttatgtgcta
      421 cacagaagaa acaatgccag taatattgac cttaatcgta attttccttt accgaacggg
      481 gaattgcatc ctgatagtaa tccctgggct ccagaaacga ttgccataat gaatcttttt
      541 aacgaacata attttgtgct tgggggtgaa tttcatggtg gtgaatgtat agtctattgt
      601 ccctggtcat acactcgttc ctccactggt gaaaatgagc ttttgtacca cattgcttcg
      661 gattattcaa tccataatcc ctatttaggg aatagtttct tttatccgaa taatattttt
      721 tgtggctcaa cttcctaccc tcttctggga actttgaatg attggggcta tgctttcacc
      781 agcagtactc aaattacctt tgaaatatac gagattaaat caccacctgc ttcagaactg
      841 cctggcttgt ggaatgataa taaagaagca atgctttact ttctggaatc ggtgcatcgt
      901 ggagtttatg gattggtgac ttctaatact ggaagacccc tgaaagcttc tatctctgtt
      961 caagattgta ataaaattgt gtattcagac cctgaggttg gtgattatta tcgtttactg
     1021 ctgcctggaa cctataatat cacagcttat gcggaaggat atgaacccca aacagtgcaa
     1081 gtaaccgttc cactatctgg ttctattgaa tataattttg cccttaatcc tgcagtaacc
     1141 gtggatttaa aaggacaatt cagagatttt gagggtaatc ttattcccta taccacagtt
     1201 aacctgaaca ccattccagc taccaatact aattttaatt ccaaggggtt ctttactttt
     1261 aatgacctta tggaaggata ttacaatatc catttcagta acaataatga ggacatctat
     1321 gaaactaatt tcttgcttac caaagagaaa aatgaagcag tttttactcc tgtagaatct
     1381 tcccttatat tttacgatcc ctgtgaagat ttgtccaact ggagtactat aggtccttgg
     1441 ggagtaacca attatcaagg agaaacagtt attaccgatt ctccttcggg taattatgct
     1501 cctgggggaa cttatacttt aactctggct aatcctatct ctctggaatt tataaagaaa
     1561 ccggtattga tgattaaagc tcggtttatt ctgataagga aatgggataa tgtctatatt
     1621 cagggctcaa cccatgcaga aaactggata gatttaggaa ttttaacggg atatggatat
     1681 gcctgggaag aaatctatta tcctctgaat cagttctctg gacataattt ttatctacgc
     1741 tttgttctcc agtccaggaa taattatggt tatgatggga tttatataga tgaaatcaag
     1801 attacaggaa ttgactatag tcaggttatt tatggtgatg tggatagtga tgatttaatc
     1861 actatgaatg atgtccaggt tctgcttcaa tatctggtag gatttgatcc agtgcccttg
     1921 attgatcctc ttccctggag tgagtatcgt atccattctg cagatgtgga tgataatgaa
     1981 gtcttggttt ctgtggatgc ctatctaatc agccgctaca ttcagaatcc caagtatcgt
     2041 tacgaagtcc agaatgggga agaacttgag ctacctcaaa tgaatatttc atttgaactc
     2101 atagaaaatc cagcagaaac agaagtatta tttcatctga atccaccaaa tgctttaaat
     2161 tgcttaaact gggaattgac tcaagccgat tctatccaaa atttaggtat agggctcatt
     2221 ccagagcaaa cttttttata tgcttttaat cctgaacaag ctaaatttgc ccttataaac
     2281 cctgaaaaag cccctctgga aagtttctct cttcatttca ataccaatct cgctaatatt
     2341 gattttctat ataatctcaa tggacaaatt ggagttattc accttcctac gggtacaaat
     2401 cttaacgata ctttaatccc tgaaatacct tttgcgctta atcaaaatta tcccaatcca
     2461 ttcaactctt tcacctgtat caagttttct ctgcctgaag agaaaaaaac cttcctggga
     2521 gtatataaca taagaggtca actggtaaag actctgatta acgaagttaa aagttcaggt
     2581 gagtatgata ttctatggga tggatgtgac aattatggac aatcagttag cagtggcgta
     2641 tatttttgta ttttgaaaag tggagatagg cttcaaactc gcaaaataat tttaattaaa
     2701 tagacctgag ggctggaaat atttaataat cgggtgagta aaaatcttta gggaatattg
     2761 gggaaagtgc gatagcatag aaaattttta aatccagata tttttcttga cataaattaa
     2821 actatattac catagtcttt ttactgtgta tgatctaaaa tttaaacggt tgaggtttat
     2881 atgtataatg taaaatggct gtattgtttt atatttactc ttgtatgttt tattattttt
     2941 ttaggtcttt tttcttgcag tagtgaagtt tctaaacctc gtactctgga agaattggaa
     3001 aaaccttttg gcattccccc ttccgagaaa cctgctaatc ctaaaagttt caatattata
     3061 gttgatgcca gtgcaagtat gtatggtttt acaggtgaaa atagtgaata tctcaatctt
     3121 cttaatacca ttatagcaaa gattcctcag gacgctaaaa tcaatttcta tagctttggt
     3181 cagggctcgc tcaagatgga aggtgactta agagccaatc tatataaatt atcccgacgg
     3241 gatttttata aagaaccaag aactgacctt accaaaccat ttaacctgat tagagaggac
     3301 aaaaattccg ttaatttaat tcttaccgat ggtgtgcaat ccactcaaca ttcacaacag
     3361 gattatgtca tatttgctaa agaattgaat tcatatctgg aaggtaatgg tttcttcgct
     3421 ttgatgggga aactagtgtc ttttgaaggg tcctattttt ctgaatatgc cagaaaatat
     3481 attgatattg aagcaggagg gaaacgtcct atatattgtc ttgccttcgg aaatcggaaa
     3541 tatgcggatt ttgtccagaa gaaaattaga gacctttttg agaattgttt tgattttgga
     3601 gtcattctac caaatcatct aaaatatacg caggatgaat cagggaaaaa tgagcctgaa
     3661 aaacttgtcc atataagtaa tgatgaaaag ttaccagtag catattttac attagataaa
     3721 ggtcattttc aatatctaaa atttaattta aagggttatg aagatagatt cggtactacc
     3781 atagattatg caattgccta tcaagcgaaa aataacccta atttcgttga attgcaggac
     3841 aaaagaggaa atattaaagc aaataaggtt gcaggaacag ataaaataac ttttaaaatt
     3901 ccttttgaag aagataatcc aggcacctat cgtatcctct tatcttttcg taaaacctta
     3961 ccctcctgga taaaagaatg gagcacggat gatgattcaa agcctgaatt ccagactaaa
     4021 acctatcatc tggctaactg gatgcaattc attctggata actttgaaga ctataaattt
     4081 ttaactacga ctcgttatta tctacaaata gagaggaaat aaatgttacc taatatctca
     4141 tttttttgga ttttcagacc caaaatttac attgaaaatt ggcataatga aatgaaccaa
     4201 agcgttttta ttgggttgat ggttaatata atttttatag ctatctatgc tgctttgcgt
     4261 taccgtagag aaaaagaaag ccaaagattc tggatggcct ttttattctc tatggttatt
     4321 aacctaattt atatcatata tactgcgatt aagatagata gtgagatggt aacggatagt
     4381 tacaggtttc tctatatcct caattttatg cttgttatgg tattgtgtga ctttttactg
     4441 ttttacctgg taagtttttt tcttagagca aaaaaatatt tttgcagann nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnga acagtgcctt actatgtatt ttctgtcggg
     4621 ggaacaggtg caagatgcct ggaatcgctt atttatttat gtgctatggg aattcattta
     4681 gaccagcctt tatttcctat atttgttgat cctgaccaga gtaatgggaa tatcaccaga
     4741 accattaaat taattcaaaa gtataaactg attagagaac atttacccag accatccaaa
     4801 gattatttat tcaatacaga gattatatac agcgacaaag aaaattcacc taatacaaca
     4861 gttaaaattc ctaatttata caatccaaac tctggtttag accctgctca aaataaactt
     4921 tcccatttta ttgaatatga aacgaaactt caggggacag attataaatt cctggctgac
     4981 cttttattca gtaaagaaga attgaatatg gatatgagtg ctggatacag aggtatccct
     5041 tctattggtt ctatcttaat gaccgatatt caaaatcaag ctttctggac agaattaaga
     5101 ggaagattgg aagaagatac tgcttctcga gtttttattt ttgcctcgtt atttggaggt
     5161 actggagctt caggatatcc agttatcagt aaattaatca aaaatgcctc tccaactact
     5221 aaggttggtg gtgttttaat gcttccctat tttaagctta aagaccctga aaacctgcaa
     5281 aaatatcaag aaagcctcaa aaaagaaaag ataatgccaa attcaaaaag ttttatgatt
     5341 aatgctaaaa ctgcttgtga attttataaa aataattatt ctcagattga ctctaattat
     5401 tttctgggag atgactttga acactgtaaa cagtacgaaa attatgctat aggcagtgct
     5461 gagcaaaaaa atgatgctca tatgctggaa ctaatggcag cttatgccgc tatagatttc
     5521 ttccatagag aaagcggcaa cttcaaacca ttctatgtaa tacaggtgaa aaatcctgat
     5581 aaaaatgctg atattaccga tgtagttgct gaagatattc ctcatcaaga taaagaaaaa
     5641 ccatttgaaa ggtttgcact actctatcat tatatcaatg aattatttga acttattgat
     5701 aaagacaatg ctaaactttt agataaaatt gcttggctaa gggaccttaa gattgatgat
     5761 aaaaaagtta gagcaagaga tataattgat aaaaaaactg aactttcttc tcttaaagaa
     5821 ttaatggatt ccttccaaga atggatttat caaaatcata ataattatgc aaaattaaat
     5881 atactggatt atgaactcaa actggataat cttctaacca acaatcctaa taaatataaa
     5941 ggctatcata taagccatct ggataccaag ttttatggta agaaaaccaa tcaaaatgac
     6001 ttattaggtg atatgttaga agcaatgtcc ttggcaaaaa taactgaagg aaggtaaaaa
     6061 atgccacgct atttactgcc aaaacataaa tctttacctc agactacacc aggtcaatgg
     6121 aaagaattaa ccataaccga ttttactacc tttgttaatg aaatggaagt ttcaccggat
     6181 tccaagatta atgttacttc cattccttca ccttgggcaa gaatgcttct ttttaaagaa
     6241 gctattaaat ctactaatca tctaatacat aaagaagtta tgtccaatat cctggatgtt
     6301 ctggagataa tcttctatta tgaattgatg aatattaata ttgagtataa agagattaaa
     6361 ataaagaatg acgagaaaaa taagtttcta aaaatactgt atgacctcag tccagaggaa
     6421 ataaagaagg atgatattgt cagcattggt ttactaattg ccagcagaga ttcaaatgat
     6481 aaattcgttt tagcaggaac cagtccattt tctctcgtat tcacacctat gaatttaaaa
     6541 cataaagagg gtaatacagt cttcccaaga tattttagag aaagacctgt ccctcttaaa
     6601 gaacgtccaa aagatttcca aaaatggata aattctaact ttctcactaa actacgaaaa
     6661 gatggaaact atcctgattt agttaaagct ttatctgatg aggaaggtat atgtgcagaa
     6721 agcttaaaag aatcattaac gcaggaaatt ttacttcctt gtgattttct cttggaatcc
     6781 tctctgggaa atctgttgga aaaaattaat accaaaaaaa tatccagctc ttatctgtta
     6841 aaacctcgaa aggaaaatga aataccccca ctggttattg acccctccaa aagtttatat
     6901 gccagtgagt attacaatgg ttataactat ctacaagatt tcaaatatga agaattaaaa
     6961 aatagcaaca gagattttct gccaggtgaa aacattaaat atccctggat attacctcaa
     7021 gaagattttt tccaaccggt tctgattaag tataaatatc gtctcaacaa taatagactt
     7081 atactcggtt cttacaaaga tagcagcgat atgaaatatg cacttcctct aactgataca
     7141 tttttcaact attttaacta tgatgatgta caggatatgt taagcatcca agaaataagt
     7201 gataaaacag ttagagttac cttaaaaatt cctatccaga atggggatta tatagaaatt
     7261 aaacgggatt attttcaaga cttaactaat agcaaagaaa atcgtattat caattatgat
     7321 gaccgggata ttgatacacc tcttccacat ataatgattt ggccccctct acatcctgat
     7381 aactggaaag atagctatta tgcttttgtt tatgggaaaa gatataaaga tggtataaat
     7441 caggaagaaa tggtgcctct ggagtttaaa gaccaggatt ttaaagatat aaattatgag
     7501 agatctcgca aagctgataa aatagaaata ttccgattag aaacacttcc tacttatatt
     7561 gtgatttctg atattgcttc caatggtaag ggtttattaa ttctcaatca taaatccctg
     7621 acaacaataa tgaatccgga aaaatcggca aaagttggta ttgattttgg aacttcccat
     7681 actaatattg ctattacaat tgataattct gaaccagaag tgctgaaata tagttctgga
     7741 tttttaggta aaaatctgaa tgataatgat tttatcaccc tcataagatt ttctgaacca
     7801 gaattaggaa gagaacaaat tcctaatttg atttataatt atttaaaaca atatttctta
     7861 cctaattcat taagtgagat tcataataat caatctgttg atatgcctct tccttctatg
     7921 atattaatgg aaggtgatac tgattcttat gaagcattat taaatacctc tattgctttc
     7981 tctaaggatg atgtaggatt gttcaattat gatttagatg gaatgacaat taaaaaaact
     8041 tatgtgcaac aaaccaattt aaaatgggaa cctcaattaa taaaacaaca agcatcaaaa
     8101 gaatatctca ggatattact gctcttgtta aagtatgaat taattaaacg aggagttgat
     8161 ttaaaaaaag tagaatatca ctgggctttt cctaaatctt tttctgaaac cttaatcagt
     8221 gattataaaa aaatgtggcg tgaattagtt ggagatgtat ataaaattac ggatgaaagt
     8281 aaagctgctt tactatattt tgaccataaa ggaattctac ctcgtaacac accagatatc
     8341 gcaataatta ttgatacagg aggtggttca tccgatattt ctatctggca agatggtcaa
     8401 atccatatgc tttacagttc tctctgggct ggaaagaatc tggttggttt tgaaaaagat
     8461 aataaaatat attcaataat ttatgacgcc atcattaatt taccttctac taagagagat
     8521 attttccaaa atattaaagg ctttcaaaat caattgaatt atattctata ttctttagat
     8581 gaaaccacat tgagtaatct ggcaaggtca gattctttct ataaaatccg ctttataatt
     8641 ctctatttct ggagtgctct actgtatgaa attggaatcc agtgcagagc aattgatatg
     8701 gataatatca aaaaaatcaa cctattcctg gctggtaatg gttcacgatt tgcctgctgg
     8761 agcagtggag cagtggataa aatggatgaa caggaacaag agatttatca aaaaataatt
     8821 aaagaaacca ttccttttaa gaatgaggta gagattcaca cttctgctgc tcaggataag
     8881 aaaagagaag tggctatagg actttgtaga ggtaatgaag aactcagagc aaaagaagca
     8941 agtaaaagac aattaattgc agaaaaagta tttattgaag cgaactctga cctatctaat
     9001 atgacaatag atgaatttaa tgaactaatt ttacagaatg gacataatat acatctggac
     9061 cagaataact ctgaaatagt caagttccat aacaccttct ttgaggtact cgcaaaaagt
     9121 aatctctata gggatagact gaaaaatgat agggctttat ctaatcttga aagtataaaa
     9181 aatatattaa ttggcgattg gggtaagttc gttggtgaat tgagaggagt tatagaagat
     9241 aatattaaaa attataattc tatttccagc tcagttttta ctcttgaaat gcagattacc
     9301 attaatagat tacatcgtta tctttcagaa aatcaataga atcagtattt aatataaaat
     9361 gagccggtta attttaggtt tagtaatttt agaagttgct gttttggctt tgcttattat
     9421 tataatctgt aaaaaattgg agaaaatgga aactgctaaa gaacctcata ggcatagtag
     9481 agatgatgca caaatccaaa acctttatac cattctcaat tcacataata caaaactggc
     9541 gaaattagag gaaatggaag aacgaattaa gcaactggag aatagattgg agcaattaga
     9601 ccagatcaaa aaacctgaac cagagaatac ggttaatccg ccaattgaaa ttcttcctcc
     9661 aaaagcaaaa gaagaaacaa ataacgataa caaaatctgg gtctggaggt ctgaagaagg
     9721 attaaaaaaa cttgaaccag ttagcaatcc caaaggtctt tatctaatca aagatggagc
     9781 aaaatatctt ctatatctgg ataatttttc taaggaaaaa atcaatgata taatgagatt
     9841 atatagggag attattgatt ttcctgccaa tattcagata aatgaccaga tagtaatgaa
//
LOCUS       DAORZF010000053         8823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_5812_length_8823_cov_421.000684, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000053 DAORZF010000000
VERSION     DAORZF010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 8823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8823
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5812_length_8823_cov_421.000684"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            216..827
                     /gene="wrbA"
                     /locus_tag="PK015_06245"
     CDS             216..827
                     /gene="wrbA"
                     /locus_tag="PK015_06245"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HOL34104.1"
                     /translation="MKILVLFYSTYGHIYEMAKAEAEGAQSVPGTEVILRRVPETLSP
                     EILERMQATEAQKKFEHIPVAQVQDLADADAIIFGTPTRFGNMSAQMKTFLDATGQLW
                     ARHSLVGKVGAVFTSTNTQHGGQESTILSFMITLLHHGMIIAGLPYTFDGQTTMDEIV
                     GGSPYGASTIVRGDNSHMPSPIELDGARYQGRYVAQLTAKLRS"
     gene            886..3375
                     /locus_tag="PK015_06250"
     CDS             886..3375
                     /locus_tag="PK015_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOL34105.1"
                     /translation="MRRFIFFILFLLSVSIFFADTSGILSTAEIAQFVDMLDSAGLTS
                     NSLKFEKDWDLSTKYKLNWQLNQLQNPWLALEDIQHIKELCDSSESGELEQLLRELGG
                     IAFNLNPYQFTTLYPSAKSVYNSQLKKEVQKPEDIFKWLDAHLKNFKEEFNQVTRDVN
                     RNRLMEFQSYWLWQFIEAEDTLTYKSYFAQKNLPEYSKLNIEKLEKEFSRLHFDLLLQ
                     SGIKFLALSDALAEHCNTLKFNNKSPIIKRTDSGYMIIGTKADDVYKDKKLSPICFLL
                     DPAGNDRYEIELKTSLENPFYIFIDLQGNDLYRNLDIASMFCSEYGFGYSLDLSGDDI
                     YQTGDFSFASFTGINLHQDKSGDDFYQSGLFSQGAAMFGLAAIIDFEGNDSYKATCMA
                     QGFGSTYGAGAILDFSGADVYYLGGKYFHEPLMPLDYLTLGQGMGFGLRPDMSGGLGL
                     LFDSKGNDKYLGGVYAQGSGYWYATGLLIDISGNDVYNTVYYPQGSGIHLACGFLLDE
                     SGDDAYYSRNGPGQGAGHDWALGIFIDSSGNDAYSIPGGNGLGLTNSVGIFIDKCGDD
                     RYERQYPQNYGYANYSRSTGGIGLFLDAGGTDTYPDTLKANNSSWYSGTYGIGRDIEL
                     NRTTKTEIEKLSEEAPLPKETDKIEEIFAAASEWEVGSAVKRVQKGREILVQRADEAI
                     PFIISNKINTQSSLEYRALETLAAKSPKFIQELYKVIDKEDSLAAKNALSLIAETGDT
                     LLLDYVERLLNQKKYETTCISVLGAINSEKSVNLLKNYIHHPSERFRYLAARSLKQIK
                     HPSAREALMLMKDDTSFLVQALLRNLPPEIN"
     gene            3375..3998
                     /gene="rsmG"
                     /locus_tag="PK015_06255"
     CDS             3375..3998
                     /gene="rsmG"
                     /locus_tag="PK015_06255"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOL34106.1"
                     /translation="MIKEKEEFIQFLQELKLSDIETIISRFENYYQLLIEYNQHINLF
                     SRATPVSNLWTHHFLDSLLPLKVIDFSNKKIMDFGSGGGLPGIPVKLAVPSCKMVLID
                     SIHKRVRATSEMILKMELSDCESICSRLEDYDTDEKFDYLLCRAVKMEKRYFGPIKRL
                     LKPEGMMLLYKAKDYSDIAALNPRELIKEDLSYGRRVIYALAPSMLR"
     gene            4021..4788
                     /locus_tag="PK015_06260"
     CDS             4021..4788
                     /locus_tag="PK015_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOL34107.1"
                     /translation="MAKIITIVNQKGGVGKTTTAVNLAAGLAVLEKKTLLVDFDPQGN
                     ATSGVGIDKDSLSLQIYDALIGKVPITETVLPTETDNLWCVPGNIDLTGAEIELVHEF
                     AREQKLREALQPVLNQYEYIIIDCPPSLGLLTVNALTAATDLLVPIQCEYYALEGVSQ
                     LLSTIRLIQKNINPSVNIIGVLLTMFDRRVNLSLQVAKEVRHYFKEKVFRTIIPRNIK
                     LTEAPSFGKPIFLYDIRSPGAMSYLNLATEIIERTQE"
     gene            4785..5639
                     /locus_tag="PK015_06265"
     CDS             4785..5639
                     /locus_tag="PK015_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOL34108.1"
                     /translation="MKEHLGRGLSALIPDKEEREDLKAHLSTLPIERIKRNPLQPRLV
                     IDDESLMELAESIKANGIIQPIIVNKTAGSDYELIAGERRLQAAKLAGLDSVPVVIRS
                     VDKKEQLQLAIVENIQRKDLDPIEEARAYQTLVEDFSLTHQEIAQIVGKDRPTISNCI
                     RLLKLPVEIQALISEGKLSSGHARAILSIDPEYQLLFAQHIIKYHLTVRQAEEKAKTF
                     VHQQEKPKSQETNPEILKSLEKDLHKILGLKVKVKERGGKGKITLEYSSPEELKHLKE
                     LLNTLRRD"
     gene            5641..6729
                     /gene="rfbB"
                     /locus_tag="PK015_06270"
     CDS             5641..6729
                     /gene="rfbB"
                     /locus_tag="PK015_06270"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOL34109.1"
                     /translation="MQRIIVTGGAGFIGANYIHHLFENPDFIGYVLNIDKLTYAGNLE
                     SLKDIEKNYPNRYFFEQVDIADTNKIASIFEQFQPDTIVNFAAESHVDRSIDSPMEFI
                     NTNIVGTAVLLENALHYWQNLDKEAQKKFRFHHISTDEVFGSLGEEGLFTESTPYDPS
                     SPYSASKASSDHLVRAWHRTYGLPVTISNCSNNYGPYQFPEKLIPLMILNCLDHKPLP
                     VYGQGLNVRDWLYVRDHCEAINYIIQQGTPGQTYNIGGHNEMRNIDIVNLICELLDEL
                     HPSPELKSYKQLITYVTDRPGHDLRYAIDSTKIQKELGWTPKETFNTGIRKTIIWYLE
                     NQDWWKNIQNKKYQQQRLGLGRNNKSST"
     gene            complement(6738..>7544)
                     /locus_tag="PK015_06275"
     CDS             complement(6738..>7544)
                     /locus_tag="PK015_06275"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOL34110.1"
                     /translation="DDIYYLQFNHFSDTKSDNSKDEFSNWKSFLTQTFSTIDSLKLHN
                     LVIDLRNNFGGNSLMGDILLSYWKLPDSLATFTCDLCISELMLKDYDMDLEDIKKILS
                     EELNREIEDFILPYVIKDMDNGVKKVSTEEYCNQQIGSLKENNMLTPLFEGKIIILTG
                     SKTFSSAVMFATMCQDNGLATIYGTPTGGKPSCFGDILSFTLPHTAIPCGVSYKYFRR
                     PDYTRDPSDSLYPDVWLEIKAEDYFSGKDSVWEKVREDIKNNTVPEPRRI"
     assembly_gap    7545..7644
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7645..8097)
                     /locus_tag="PK015_06280"
     CDS             complement(<7645..8097)
                     /locus_tag="PK015_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34111.1"
                     /translation="MKIQLCWWLVIVFLFIGILNATTESVSAELSYNIFQQDFDFFQK
                     TLRESHPNIYANLPEPEFNKQVNLIIEELATETDTLHFLAKLKQFSHLLGDAHTGPFY
                     YQKETLFYPIAVCWLNDSWYINGTMKELADNIGSKIVSINGKDAEEFMS"
     gene            complement(8173..8784)
                     /locus_tag="PK015_06285"
     CDS             complement(8173..8784)
                     /locus_tag="PK015_06285"
                     /inference="COORDINATES: protein motif:HMM:NF024686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FN3 associated domain-containing protein"
                     /protein_id="HOL34112.1"
                     /translation="MKNFRFILILVLVLFSLSCSKKTTELIQLDAPIFNPGSGTYLAG
                     QAIYITCPEYGASIYYTVNGSDPTQNDVLYDRPLIIPNFFPEGANSATIKARAYKEGF
                     DPSNVSTATYFVSYYNTVATPIISPVGGNITTETIITIVCPTYEAQIYYTLDGTEPTQ
                     NSIHYSEGFTISQTGEVTLKARAFRQNWNPSEIAVANYVVSNP"
BASE COUNT         2812 a         1537 c         1660 g         2714 t
ORIGIN      
        1 agagctaaga aagagccaag agagagccaa taaaaaaaga aaagtgttat acatttgata
       61 atataaagct gaatgttttt gaacaacact aataattata tatctatcgc aaattattac
      121 tattatcctg gtttatttaa aaggaagggt gaatttttct cttggacaat gatgctattc
      181 cggttactat tttgctataa gaggaggttg ataaaatgaa gatattagtg cttttttatt
      241 ccacctatgg acatatctat gaaatggcaa aagcagaagc agagggagca cagagtgttc
      301 ctggaacgga agtgatttta agaagggtgc cagaaacttt gtcccctgag attttagaaa
      361 ggatgcaagc aacggaagct caaaagaaat ttgaacatat tcccgtagca caagttcaag
      421 atttagctga cgcagatgcg attatctttg gcactcccac tcgttttggt aatatgtcag
      481 ctcaaatgaa gacttttctt gatgccacag gacagctttg ggctcgtcat tctttagtag
      541 gaaaagttgg tgctgttttt accagtacaa acactcaaca tggagggcag gaatcaacta
      601 tcttgagctt tatgattacc ttacttcatc atgggatgat tatagcaggc ttgccctata
      661 cttttgacgg tcaaaccaca atggatgaaa ttgttggtgg ctctccttat ggagcttcta
      721 ccattgtaag aggggataat tctcatatgc cttctcccat agaattggat ggtgctcgtt
      781 atcaaggaag atatgttgcc cagttaactg ctaagcttag aagctgattg ttattgacac
      841 aaattcaggc tccttattaa atgttttatg ataaggagct tttttatgcg tcggttcatc
      901 ttttttatac tcttccttct ttccgtcagt atattctttg ctgacacctc gggaattctt
      961 tctactgcag agatagcaca atttgttgat atgcttgatt ctgcaggatt aacttctaat
     1021 tctttaaagt ttgaaaagga ctgggattta agcactaaat ataaattaaa ctggcagtta
     1081 aatcaattac agaatccctg gttagctctg gaagacatac agcatattaa agaattgtgt
     1141 gattcctcag aaagtggtga attggaacaa ttgcttcgcg aattaggagg cattgctttc
     1201 aatctcaatc cttatcaatt tactacgctt tatccctcag ctaaatcagt ttataatagc
     1261 cagctaaaaa aagaggtaca aaagcctgaa gatatattta aatggctgga tgcacatcta
     1321 aagaatttta aagaggaatt taatcaagtt acacgcgatg tgaatagaaa caggcttatg
     1381 gaattccaaa gttactggtt gtggcagttc attgaagcag aggatactct tacctataaa
     1441 tcctactttg ctcaaaagaa tcttcctgaa tatagcaaac taaatataga gaagctggaa
     1501 aaggaattct cccgtttgca ttttgaccta cttttacaat ctggaataaa atttctcgct
     1561 ctttctgatg ctttagctga acattgcaac accttgaagt ttaataataa aagccccata
     1621 attaagcgaa cagattctgg ttatatgatt attggaacta aggcagatga tgtctataag
     1681 gataaaaaac tttctcctat ctgttttttg cttgacccag cgggaaatga ccgttatgaa
     1741 attgagctta aaaccagtct ggaaaatcct ttttatatct ttattgacct tcaaggaaat
     1801 gacctgtata gaaatcttga tattgcttct atgttttgca gtgaatatgg ttttggttat
     1861 tcactggatt taagtggaga tgatatttat cagacgggcg atttttcttt tgcttccttt
     1921 acagggataa atcttcatca agataaatct ggcgatgatt tctatcaaag tggtctattc
     1981 tctcagggag cagcaatgtt tggcttggca gctataattg attttgaagg caatgatagc
     2041 tataaagcta cttgcatggc acagggtttc ggcagcactt atggtgctgg tgcaattctg
     2101 gatttttccg gagcagatgt ctattatttg ggaggaaaat actttcacga accgctaatg
     2161 cctctggatt atctaacctt gggacaaggt atgggttttg gtctccgtcc tgatatgtca
     2221 ggagggttag gtttactttt tgatagtaag ggcaatgata aatatctggg tggtgtttat
     2281 gctcaaggtt caggttactg gtatgcgacg ggtcttttga ttgatatatc tggaaatgat
     2341 gtctataata cagtctacta tcctcaaggt tctggtattc atcttgcttg tggtttctta
     2401 cttgatgaat ctggcgatga tgcttactat agtagaaatg gacctggaca gggtgctggt
     2461 cacgattggg ctttaggtat ctttatagat agttcgggaa atgatgctta ttccattcct
     2521 ggtggaaatg gtctcggatt aactaattct gtgggaattt ttattgataa atgtggtgat
     2581 gaccgctatg aacggcaata tccacaaaat tatggctatg ccaattactc tcgtagcact
     2641 ggtggaatag gtctatttct tgatgcaggt ggaactgata cctatccaga cactttaaag
     2701 gctaataata gcagctggta tagcggaacc tatggtatag gtcgggatat agaattgaat
     2761 agaacaacta aaactgagat agaaaagctg tctgaagaag caccattacc taaagaaaca
     2821 gataaaatag aagaaatatt tgctgctgct tcagaatggg aagttggctc tgccgttaaa
     2881 agagttcaaa aaggccgtga aatcttggtt caacgagcag atgaagctat cccctttatt
     2941 atctctaata aaattaatac tcaatcttca cttgaatacc gtgctctgga aactctggca
     3001 gctaaatctc caaaatttat ccaggaacta tataaagtga tagataaaga agatagttta
     3061 gcagcaaaga atgcactctc tctaattgct gaaacgggag ataccttgct cttagattat
     3121 gttgagagac ttctaaatca gaaaaaatat gaaactacct gtatttccgt tttgggtgct
     3181 ataaatagtg aaaaaagtgt gaatctgctt aaaaactata tccatcatcc cagtgagaga
     3241 tttcgttatc ttgccgccag gtccttaaaa cagattaaac atccctctgc tcgggaagca
     3301 ttaatgctga tgaaagacga cacttcgttc ttagttcaag ctttgcttcg taatctccct
     3361 ccagaaataa attgatgatc aaggaaaaag aagaattcat tcaatttttg caagaactta
     3421 agctttcaga tatagaaacg attatttcca gatttgaaaa ttattatcag ttactaatag
     3481 aatataatca acatattaat ttgttttctc gtgccactcc tgtttctaac ctgtggactc
     3541 accattttct tgacagtctt cttcctttaa aagttattga tttctcaaat aagaaaataa
     3601 tggattttgg atcgggtggt ggtttaccag gaattccagt taaactggcg gttccttctt
     3661 gcaagatggt gctcatagat tctattcata aaagagtaag agccactagt gaaatgattc
     3721 ttaagatgga gttatctgat tgtgaatcaa tatgtagtcg tctggaagat tatgatacgg
     3781 atgaaaaatt tgattacctg ctctgccgtg cggtaaaaat ggaaaaacgc tactttggac
     3841 caataaaaag attgcttaaa ccagaaggga tgatgcttct ttataaggct aaggattata
     3901 gtgatatagc tgccttaaac ccgagagagt taataaaaga agatttatcc tatggtagac
     3961 gtgttattta tgctcttgca ccctctatgc tcagataata aaacttaaat ataatatact
     4021 atggctaaaa taattacaat agttaaccaa aagggaggag tgggaaaaac cactactgcc
     4081 gttaatcttg ccgctggatt ggcagtgctg gaaaaaaaga ccttactggt agattttgat
     4141 cctcagggta atgccaccag tggtgtaggt atagataaag atagtctttc tttgcaaatc
     4201 tatgatgctt taattggtaa agtccctatc acagagacag ttcttcctac agaaacagat
     4261 aatctctggt gtgtccctgg aaacatagac ctcacgggag cagaaattga actggttcat
     4321 gaatttgcca gagaacaaaa actaagagaa gccctccaac ctgttttaaa tcaatacgaa
     4381 tatatcatca tagattgtcc cccttctctt ggacttttaa ccgtaaatgc tttaaccgct
     4441 gcaacagacc tcttagttcc tatccagtgt gaatattatg ctttggaagg cgtcagtcag
     4501 ttactttcca ctatccgatt gattcagaaa aacataaatc cgagtgtcaa tataattggc
     4561 gttcttttaa cgatgtttga ccggagagta aatctttcct tacaggtagc caaagaggtg
     4621 cgacactatt ttaaagagaa ggttttccgc acaattattc cgcgtaacat caagctaacc
     4681 gaagctccca gttttggtaa acctatattt ttatatgata tccgctctcc tggggctatg
     4741 agttatctca atcttgccac cgaaattatt gaaaggacac aggaatgaaa gaacatcttg
     4801 gacgaggctt aagtgcttta attcctgata aagaggaaag agaagacctc aaggctcatt
     4861 tgagtaccct acctattgag cgaataaagc gcaatccctt acagccaaga ctggtgatag
     4921 atgatgaaag cttgatggaa ctggcagaat ctattaaagc taatggtatc atccagccga
     4981 taattgtgaa taaaactgcc ggttcagatt atgaactcat agctggagaa cgcagattac
     5041 aggcagcaaa actggcagga ctggactcgg ttccagtagt tatacgtagt gtagataaaa
     5101 aagaacagct ccaacttgct atagtggaaa acattcagag aaaagattta gaccctatag
     5161 aagaagcccg tgcctatcaa actctggtag aagatttctc tctcactcat caagaaatag
     5221 ctcaaattgt aggtaaagac cgtcccacca tctctaattg tatcagattg cttaaactac
     5281 cagtagaaat ccaagctctg atctcggaag ggaaactaag ttcaggtcat gcacgtgcca
     5341 tcttaagtat tgaccctgaa tatcaacttt tatttgctca acatattatc aagtatcatt
     5401 taactgttcg ccaggcagaa gaaaaagcta agacttttgt tcatcaacag gaaaagccga
     5461 aatctcaaga aaccaatcca gagatattga aaagtctgga aaaggattta cataaaattc
     5521 tggggttgaa agttaaagtg aaagaacgag gtggaaaggg aaaaattacc cttgaatatt
     5581 cttcaccaga agaacttaaa catttaaaag aactattaaa cactctccgg agggattgag
     5641 atgcaacgca taattgtcac gggtggtgca ggttttattg gtgctaatta tattcatcat
     5701 ctatttgaaa acccagattt cattggttat gttctcaata tagataaact tacctatgcg
     5761 ggaaatctgg aaagcttaaa ggacattgaa aagaactatc ccaatcgcta tttctttgaa
     5821 caggttgata tcgcagatac gaataaaata gcttctatct ttgaacaatt tcagccagac
     5881 accattgtta attttgctgc tgaatctcat gtagataggt ctattgatag tcctatggag
     5941 ttcataaata caaacattgt cggaactgcc gtgcttttag aaaatgcttt gcattactgg
     6001 caaaatttag ataaagaagc tcagaagaag ttccgcttcc atcatatttc tacagatgaa
     6061 gtctttggtt ctttaggaga agaaggattg ttcacagaaa gcactcctta tgacccttct
     6121 tcaccctatt ctgcttccaa agcaagttct gaccatctgg ttagagcctg gcatcgcacc
     6181 tatggacttc ctgttactat ttctaactgt tccaataatt atggacctta tcaattccca
     6241 gaaaaactta ttcctctgat gattcttaat tgtcttgacc acaaaccttt acctgtttat
     6301 ggtcaaggct taaatgtgag agattggctg tatgtgcgag accattgtga agctattaat
     6361 tatattatcc agcagggaac tccaggtcaa acttataata tcggaggaca taacgagatg
     6421 aggaatatag acattgtaaa ccttatctgt gaacttctgg atgaactgca tccttcacct
     6481 gagttgaaat cctataaaca attgataact tatgtaacag accgccctgg acacgattta
     6541 cgttatgcta tagattcaac taaaatccag aaagaattag gctggactcc taaagaaact
     6601 tttaatactg gaattcgtaa aacaattatc tggtatctgg aaaatcaaga ctggtggaaa
     6661 aacattcaaa ataagaaata tcaacaacaa agattgggac tgggaagaaa taataaatca
     6721 agcacctgaa attcttatta aattcttctg ggttcaggca ccgtattatt ttttatatct
     6781 tctctgactt tttcccaaac agaatccttc ccggaaaaat aatcttcagc tttaatttct
     6841 aaccacacat ctggataaag actgtctgaa ggatctctgg tataatctgg acgacgaaaa
     6901 tacttatatg aaacaccgca tggaatagcg gtatggggta aagtgaagga taaaatatct
     6961 ccaaaacaag aaggtttccc acctgtgggt gttccataaa tagtggctaa tccattatcc
     7021 tgacacatag tggcaaacat tactgcagaa ctaaaggttt tgcttccagt tagaataata
     7081 attttcccct caaaaagagg agttagcata ttattttctt ttaaagagcc tatttgctga
     7141 ttacaatatt cttccgttga tacttttttt actccattgt ccatatcttt aataacatag
     7201 ggcaaaataa aatcttctat ttctctattc aattcttctg aaagaatctt ttttatatcc
     7261 tctaaatcca tatcataatc cttcaacatc aattcgctga tacataaatc acaggtaaag
     7321 gtagctaaag aatctggtaa cttccaatag gaaagaagaa tatctcccat taaagaatta
     7381 ccaccaaagt tatttcgtaa atcaatgacc agattatgta atttaagact gtcaatggta
     7441 ctaaaagttt gagttaaaaa agacttccag ttggaaaact catctttgga attatcgctc
     7501 ttggtatctg aaaagtggtt aaactgaaga taatatatat catcnnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnagacat aaactcctct gcatctttcc catttatgct
     7681 gactatttta cttcctatat tatcagctaa ttctttcatc gttccattaa tataccagct
     7741 atcattcaac cagcatactg caataggata aaatagagtt tccttctggt aataaaaagg
     7801 accggtatga gcatcaccta aaagatgaga aaactgtttt aatttggcta aaaaatggag
     7861 ggtatctgtt tccgtggcta attcttctat tataagattg acttgcttat taaactctgg
     7921 ctcgggtaaa ttagcataga tattgggatg cgattctcgc aaggtctttt gaaaaaagtc
     7981 aaagtcttgt tgaaagatgt tatatgagag ctcagcagaa acagattcag tggtagcatt
     8041 aagaatgccg ataaaaagaa atactatcac taaccaccaa cataattgga ttttcatagt
     8101 atggattagt cctttaattt tttacagtag ttctaagttc ttttgataaa gatagataat
     8161 ttatcctatc aatcagggat tacttaccac atagtttgct acggctattt cacttggatt
     8221 ccagttttgc ctgaaagctc tggctttaag agttacttca cctgtttggg atatagtaaa
     8281 tccttctgaa taatgtatgg aattctgggt aggttctgtc ccatcaagag tgtagtatat
     8341 ctgtgcttca taagttgggc atacaatagt aataattgtt tctgtagtga tatttccacc
     8401 tactggagaa ataataggag ttgccacggt attataataa gatacaaaat atgtggcagt
     8461 agaaacatta ctgggatcaa atccctcttt atatgctctg gctttaatgg ttgcagaatt
     8521 agcaccctct ggaaagaaat tcggaataat tagcggtcta tcatataaaa catcattttg
     8581 cgtgggatca gaaccattaa ccgtataata tatggatgct ccatactcag gacaggtgat
     8641 atagatagct tgaccagcta aataagtccc gcttccagga ttaaatatcg gtgcatccag
     8701 ttgaattagt tctgtcgttt ttttagagca agataacgaa aataacacca agactaatat
     8761 aagaataaag cgaaaattct tcataattca cctctttcac tttttcattt atctcataat
     8821 aaa
//
LOCUS       DAORZF010000054         8444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_6102_length_8444_cov_411.815353, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000054 DAORZF010000000
VERSION     DAORZF010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 8444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8444
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6102_length_8444_cov_411.815353"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..580
                     /locus_tag="PK015_06290"
     CDS             83..580
                     /locus_tag="PK015_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOL34113.1"
                     /translation="MKKEQFPEFLKANEKRIYNYLLSLLGDESDANDVAQMVFLSFYE
                     HIDNIDESTALSYLYRIAHNKSMTYLKQKNRYVNFEPQSFEQMQDRSNKGNEKDYSLL
                     HSAIKELPPRLASVIQLQYFDKLSYKEIAEKLGISVKAVESLLVRAKKRLRKKLMKDR
                     MEGRV"
     gene            626..1099
                     /locus_tag="PK015_06295"
     CDS             626..1099
                     /locus_tag="PK015_06295"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOL34114.1"
                     /translation="MRCSKAQFLIELKLDNELPKEKGKALKLHLENCPQCRKYEAEAR
                     KLQQLLSTFPVREFPSWLHNSILEETVKHERKRMLNSYIVRWQTIPALIAIVLSLIGG
                     WALGKTAYIRVNPLPENASVIYSNQVSQTDSLNLVVFGESTLMEDLFYQISEDYK"
     gene            1096..1551
                     /locus_tag="PK015_06300"
     CDS             1096..1551
                     /locus_tag="PK015_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34115.1"
                     /translation="MSKRVLTILLLISLFLNAGIIGGLIVMNVFRHNHIEHHYQRPPD
                     GPPPNNKGRFAPPDRFDPQIVALRDSFIKTKRELMEELSKDPVDEAKVKAIIEKSVEA
                     QSKMERAMGYKMLEWRKTMSAQEAKELFSSKVARLDTQGEKNNQRRKAK"
     gene            1548..2108
                     /locus_tag="PK015_06305"
     CDS             1548..2108
                     /locus_tag="PK015_06305"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HOL34116.1"
                     /translation="MKKTFIITLIALMLIGIIWAKDTAKTMPPSTVKSNKTSQMNATL
                     ELNPRSEQVRFKNALKELNLTEAQKKKLDELQLNHRKAMNTINAEIRNLQLDLHKALK
                     EEDYTTAKILNSQLYEKKRIRANNLIEFREQIMKELTPEQKTKMQNLFPGSGLGLGPR
                     DGKFSPNIDRCPNADTNHLGCGKNCK"
     gene            complement(2279..3718)
                     /locus_tag="PK015_06310"
     CDS             complement(2279..3718)
                     /locus_tag="PK015_06310"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOL34117.1"
                     /translation="MEQIIKQTIQKLSLPEITFADVRITRTDNEGIFFQNGFLRGYST
                     NLDSIALGIRVLINGCWGFAGTRDLSSSSIDKAIHTAISNAHFGSLFQKTKVEFPPLS
                     PVQAEYEHHPEQDPFKMAKEEKIDYLQRLAEAIKPQEKIVHSIIYAAFMRQEKYYANS
                     EGTYAHYTFYNTLPMMSVLASDGAQVQSRTWPGHMGAGRAGFEIFHKHKFEENTERII
                     KEATDLLSAPTIEEDKADIIIGSGHLALQLHESVGHATEADRIFGMEISYAGKTFIKP
                     EMIGSFQYASPVVNIFSDSTDPRGMGYHPVDDEGVPGKKVDIIKNGILVNQQTSRQIA
                     PLLGLEPSSNMRASYGDDFPLVRMTNFCLAPGKGTLDELIQDTEHGYLLDFTKTWSID
                     DNRNNFQFTTEIGYKIENGKIVGIVKEPTYFGITKEFWNSCDRVCGPEEWDYQSTFNC
                     GKGEPGQAMQLSHGVAPARFRNINVRVKI"
     gene            complement(3729..5054)
                     /locus_tag="PK015_06315"
     CDS             complement(3729..5054)
                     /locus_tag="PK015_06315"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOL34118.1"
                     /translation="MENLEYLKSLLDIHPELQYEIIEQYWTTDFLRFYHSQTNYNISK
                     ETTTLSATIYKGKKSFNFMLDNATSEKIDNAVHNALTIIDKLPEDPYFVDLENDKTLA
                     KPREVKNNIEAIPLKLKTEILSRIAQAVAPHNFDIFGSFVCNYQKYRLINSNGLDKTS
                     ENSPYYLEVKAVHNKSQVTVIEAFGGEDFQYFQEQNFIEHLLQKIGYCFNDIVDIEPG
                     TYDVILAPRCLGEFAQYLTYGMHARSLDQHSSFFEGKLNQKVFPEWISICDDPDDPEI
                     IHKDYGSNGHIYRYLSLVDNGIFRNFMCDNYYSHKTGLPKNGNTGSCLKIEAGDKTLD
                     EMIGSVKHGLFISSLHYMNFINPKETSITGLTRDGTFLIENGKITKVVNNLRFTEKVA
                     RIFEHIQALENRSHPVPFSGNYEIFDIETVKSPHALVRDFNISSSTKTI"
     gene            complement(5078..6346)
                     /locus_tag="PK015_06320"
     CDS             complement(5078..6346)
                     /locus_tag="PK015_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOL34119.1"
                     /translation="MKLFTNVSLPTSSAAFKRVNVLFNETIIKISSDEIQSDELTEII
                     DLKGKILLPGAVDAHSHIITEKDAEKNLAHITKSALLGGWTTFAELSYFNPKPIFHLE
                     DIKYWQDLIEKNSFLDMALWGNVNIASYPYHAEAAQELWSQGIVGFALLHPSPNKHIP
                     TISLPEIMDLFLDIYESDTLFAFQGYDFEDDQEFSFQSQDNAIKKLLRRIQENPIHLP
                     YISYYPTLEFLNSIYKRTDISYALNIADLMKFFSGIDLGQEIELDDPEHQLFELLRTN
                     KIYMLSNNVEASSVPENVSLAFRGTTEKLLPYSYLWTLSELWKKRKVPLATVIKMTSE
                     NSAKRLGLYPTKGCIAAGSDADFVIYNPEKETLFKAPDGSINKLEGSIEEVYLRGNRV
                     VQKGKAATASGSFLFRKNSPKRRYNTTSWI"
     gene            complement(6650..6997)
                     /locus_tag="PK015_06325"
     CDS             complement(6650..6997)
                     /locus_tag="PK015_06325"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOL34120.1"
                     /translation="MKKILFVIAMGLLMLTMLSAKEMKDYTEIVIDPNPMDNYTNITV
                     SFSSPVEVQISIETGSGKVIKTLYSGMIKNYLRIGWDRIGDDGSYTPSGEYYLVVRYN
                     QRFTSIKKTMILR"
     gene            complement(7043..7987)
                     /locus_tag="PK015_06330"
     CDS             complement(7043..7987)
                     /locus_tag="PK015_06330"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOL34121.1"
                     /translation="MSTLDKYFLGIDIGASSVKYGWGNSKVGLQYFNKKELSTKTLPF
                     LKNIISEILNFASDNPIWQKIKAIGIGTPGTIDQNIHQIVGVNPNLPFLVNLNIENLI
                     PENIKIPVYWDNDANLMCFAEAWLRDFKCDIVGITIGSGIGCGFVQKDRIFHGSHGYA
                     MELGHICIIPGGELCSCGRKGCVEAYSSVDGLKRRISLLEIPFSKTESSLNVKDLLYL
                     TAIDSKAKQILEEGLYILAQGITNLSVLLDPEIIVIGGGAMEGSLYDWKKLQKQIIDF
                     MPAINAEHTRIEKALAGNKAGVLGAIILAESVNKFVSE"
     gene            complement(7997..8072)
                     /locus_tag="PK015_06335"
     tRNA            complement(7997..8072)
                     /locus_tag="PK015_06335"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8037..8039),aa:His,seq:gtg)
     gene            complement(8103..8179)
                     /locus_tag="PK015_06340"
     tRNA            complement(8103..8179)
                     /locus_tag="PK015_06340"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8143..8145),aa:Arg,seq:tct)
     gene            complement(8186..8262)
                     /locus_tag="PK015_06345"
     tRNA            complement(8186..8262)
                     /locus_tag="PK015_06345"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8226..8228),aa:Met,seq:cat)
     gene            complement(8314..8389)
                     /locus_tag="PK015_06350"
     tRNA            complement(8314..8389)
                     /locus_tag="PK015_06350"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8354..8356),aa:Met,seq:cat)
BASE COUNT         2731 a         1541 c         1530 g         2642 t
ORIGIN      
        1 agaccaaata tcttgacatc atttctaaag gataatttga agggtctaat ccctttttgg
       61 ctaaaaatta taaggtaata taatgaaaaa agagcagttc ccagaattct taaaagctaa
      121 tgagaaaagg atatataact atttgctttc ccttttaggg gatgaaagtg atgctaatga
      181 tgttgcgcaa atggtctttc tatcctttta cgaacatatt gataatatag atgaatctac
      241 tgctctttcc tatctgtatc gcattgctca taataaaagt atgacctatc tgaaacagaa
      301 gaatcggtat gttaattttg agcctcaatc ctttgaacaa atgcaagatc gtagcaataa
      361 gggaaatgag aaagattatt ccttacttca ttcagccatt aaagaacttc caccccgatt
      421 agccagtgtt attcaactgc aatactttga taaacttagc tataaagaaa tagcagaaaa
      481 attgggcata agtgtaaaag ctgtagaatc attattggta agagctaaga aacgactgcg
      541 gaaaaaatta atgaaggata gaatggaagg aagggtatag agttatgaaa aagattaaaa
      601 gaatgaaatt tgtaaagagg caaccgtgag atgcagtaaa gctcagtttc tgatagaatt
      661 aaagcttgat aatgaacttc caaaagagaa aggaaaagct ctaaagctgc atctggaaaa
      721 ttgtccccaa tgccgtaaat atgaagcgga agccaggaaa ttacagcaat tattaagcac
      781 ctttcctgtt cgtgaatttc cttcttggtt gcataatagt attctggaag aaacggttaa
      841 gcatgagcgg aaaagaatgc tgaacagtta tattgttaga tggcaaacca ttcctgctct
      901 tatagcaata gttttaagtt tgataggagg ttgggcatta gggaaaactg cttatattag
      961 ggttaaccct cttccagaga atgcttccgt aatctacagc aatcaggtat cacaaacgga
     1021 tagtttgaat ctggtagtct ttggagaaag caccttgatg gaagatttat tttaccaaat
     1081 aagtgaggat tataaatgag taaacgcgtg ttgactattc ttttacttat atcgcttttt
     1141 cttaatgccg gaatcattgg tggtttgata gttatgaatg tatttcggca caatcatata
     1201 gagcatcatt accaaagacc tccggatgga cctccaccta ataataaggg aagatttgct
     1261 ccaccagata gatttgaccc tcagattgta gccttgcgtg atagtttcat taaaactaaa
     1321 agggaattga tggaagagct atctaaagac ccagtagatg aggcaaaagt caaagccata
     1381 attgaaaaat cggtggaagc tcaaagcaag atggaaagag ctatgggcta taaaatgctg
     1441 gaatggcgga aaactatgag tgctcaagaa gcaaaagaac ttttttcctc aaaagtagca
     1501 agactggaca ctcagggaga aaaaaataat caaaggagaa aagcaaaatg aaaaagacct
     1561 ttattatcac actaatcgct ttaatgttaa taggcataat ctgggctaaa gatacagcta
     1621 aaactatgcc accttcaacg gttaaaagta acaaaacttc tcaaatgaat gccactttag
     1681 aattaaatcc tcggtctgag caggttaggt tcaagaacgc actaaaagag cttaacttaa
     1741 ccgaggcaca gaaaaagaaa ctggatgaat tgcaacttaa tcatagaaaa gcaatgaata
     1801 caattaatgc agaaattcgg aaccttcagc ttgatctgca taaagctttg aaagaggaag
     1861 attacactac tgcaaaaata cttaatagcc agctttatga gaaaaaacgc atccgagcta
     1921 ataacctgat tgaatttaga gaacagatta tgaaagagct aactccagag cagaaaacta
     1981 aaatgcaaaa tctatttccc ggtagtggtt taggattagg tcctcgtgat ggaaaatttt
     2041 cacccaatat tgatagatgt cctaacgcag atacaaatca tttaggttgt ggcaaaaatt
     2101 gtaaataatc aagagaggaa aaaattttaa aaaataactg accatagctt ttaacctctc
     2161 taaagtgaaa tctaaataaa aggtgcgtcg taagataaaa ctttacgacg caccattttt
     2221 ttatatagtg tttctttctg caggttaatt atgaagcagc agaaagctta ttctgctctt
     2281 atatttttac ccgaacatta atatttctga atctggcagg agcaactcca tgagataatt
     2341 gcattgcttg tccgggttct cctttaccac aattgaaggt gctttgataa tcccattctt
     2401 caggaccaca aacacgatca caggaattcc agaattcctt agtgattcca aaataggttg
     2461 gttctttaac aattcccact atctttccgt tttcaatttt gtatcctatt tctgtggtaa
     2521 actggaagtt attacgatta tcatctatac tccaggtttt ggtgaaatca agtaaataac
     2581 cgtgttcagt gtcctggatt aactcatcta aagtgccttt tccaggagca agacagaaat
     2641 tagtcatccg caccaagggg aaatcatctc cataggaagc tctcatatta gaagaaggtt
     2701 ccagaccaag tagaggagct atctgacgcg aagtttgctg atttaccagg attccatttt
     2761 tgattatatc cactttcttt cctggaactc cctcatcatc aacaggatga tagcccattc
     2821 cacgagggtc tgtgctatca ctgaagatat tcactactgg agaagcatac tggaaagagc
     2881 caatcatttc aggcttaatg aaggttttac cggcatagga tatctccata ccaaagatac
     2941 gatctgcttc tgtggcatgt cctacggatt catgtaattg gagagcaaga tgaccacttc
     3001 caataatgat atctgcttta tcttcttcaa tagtgggagc agacagtaag tcagtggctt
     3061 ctttgattat gcgttcagtg ttttcttcaa atttatgttt atggaatatc tcaaatccag
     3121 ctcttccagc acccatatgt ccaggccaag tgcgagattg cacttgagct ccatctgaag
     3181 ctaagactga catcatagga agggtattat aaaaagtgta atgagcatag gtgccttcac
     3241 tattagcata gtatttttcc tgacgcataa aagcagcata aatgatagaa tgtacaatct
     3301 tttcctgtgg tttaatagct tctgccaatc tctgaaggta atcaatcttt tcctcttttg
     3361 ccattttaaa aggatcttgt tcaggatgat gttcatattc cgcttggaca ggagaaagag
     3421 gagggaattc tactttagtc ttctggaaaa ggcttccaaa atgggcatta gagatagcgg
     3481 tatggatagc tttatctatg gaagaactac ttaaatctct ggttccagca aagccccagc
     3541 aaccatttat caacactcta atacccaaag caatagaatc aaggttggta ctataacctc
     3601 tgagaaagcc attctggaaa aagatgcctt cattatcggt gcgagtgata cgtacatcag
     3661 caaaagtgat ttcgggaagc gataattttt gtatggtttg ttttattatt tgttccattt
     3721 tattaccctc agattgtttt agtagatgaa gatatattga aatctcgcac taaagcatgg
     3781 ggagatttca ccgtttcaat atcaaagatt tcataatttc cagaaaaagg aacaggatga
     3841 ctgcgatttt ccagagcctg gatatgttca aagatacggg caactttttc cgtgaaacga
     3901 agattattta ctactttagt aatcttccca ttttcaataa gaaatgttcc atctctggtt
     3961 aaaccagtta tagaggtttc tttaggattg ataaaattca tatagtgaag actggatata
     4021 aacagtccat gtttgacgct acctatcatt tcatccagag tcttgtctcc tgcctcaatt
     4081 tttaaacaag agccggtatt tccgttttta ggaagtcctg tcttgtgtga ataatagtta
     4141 tcacacataa agttacggaa tattccattg tccaccagag aaagataacg atagatatga
     4201 ccattagaac cgtaatcctt atgaattatt tccgggtcat ccgggtcatc acagatgcta
     4261 atccattcgg gaaatacttt ttgattgagt tttccttcaa agaaactact gtgctggtct
     4321 aaagagcgtg catgcatacc atatgtaagg tattgagcaa attcacccag acagcgtgga
     4381 gcaagaatga catcataagt tcctggttca atgtccacaa tatcgttaaa acaatagcca
     4441 attttttgta ataaatgttc aataaagttc tgttcctgga aatattgaaa gtcttctccc
     4501 ccaaatgctt ctatcacggt cacttgagac ttattatgaa cagctttaac ttccagataa
     4561 taaggagaat tctcactggt tttatctaat ccattgctgt taatgagtct gtatttttga
     4621 tagttacaga caaaggagcc aaatatatca aaattatgtg gtgcaacagc ttgtgcaatt
     4681 cttgataaga tttctgtttt caattttaag gggattgctt caatattgtt ttttacttct
     4741 cttggttttg ccagggtttt gtcattctcc aagtctacaa aataagggtc ttcgggcaat
     4801 ttatctatta ttgtcagggc attgtggaca gcattatcaa ttttttcaga agttgcatta
     4861 tccagcataa agttaaatga ttttttaccc ttatagatag ttgcactaag cgtggttgtt
     4921 tccttgctga tgttgtaatt ggtctggcta tgataaaagc gcaggaaatc cgttgtccag
     4981 tattgttcaa taatctcata ttggagctca ggatgtatgt ccagcaggct tttcaggtat
     5041 tccagatttt ccatttatcc tccagttaat atatttttta aatccaggaa gtggtattat
     5101 atcgtctctt aggggaattt ttacgaaaga gaaaagaacc actggcagtt gctgctttcc
     5161 ctttttgaac cactctattt cctcttagat atacctcttc tatgcttcct tccaatttat
     5221 ttatactgcc atcaggtgcc ttaaacagag tttctttttc cgggttataa atcacaaaat
     5281 cagcatctga gccagctgct atacaacctt tagtgggata tagtcccaat cttttagcag
     5341 aattttcact agtcattttt atcactgtag ccagtggaac ctttcgcttt ttccatagtt
     5401 ctgagagtgt ccagagatag gaatagggta acaatttttc cgttgtaccc cgaaaagcta
     5461 aactcacatt ttcaggaaca gatgaagctt caacattatt ggaaagcata tagattttat
     5521 tggtgcgtaa aagttcaaaa agttgatgtt ccgggtcgtc cagctcaatc tcttgaccaa
     5581 ggtcaatgcc agagaaaaac ttcatcaaat ctgcaatatt tagggcataa gagatatctg
     5641 tgcgtttata gatactgttc aaaaattcca aggtaggata atatgaaata taagggagat
     5701 ggataggatt ttcttgaatg cgtcgtaaaa gtttctttat agcattatcc tgggattgga
     5761 atgagaattc ctggtcatct tcaaaatcat aaccttgaaa agcaaagaga gtatcagatt
     5821 cgtaaatatc cagaaaaagg tccattattt ctggaagaga tatagtagga atgtgtttat
     5881 tgggagaagg atgtaaaagt gcaaaaccta ctattccctg agaccataat tcttgagctg
     5941 cttcagcgtg ataaggatag gaggcaatat taacatttcc ccaaagtgcc atatccaaaa
     6001 aggaattctt ttctatcaga tcttgccagt atttaatatc ttctaagtgg aaaatgggct
     6061 ttggattaaa gtaactcagt tcagcaaaag ttgtccatcc acctaataat gcggacttag
     6121 tgatatgagc aaggtttttt tcagcgtctt tttcagttat aatatgactg tgggcatcaa
     6181 ctgctcctgg aagtaatatt ttccctttaa gatctataat ctcggttaac tcatctgatt
     6241 ggatttcatc actggaaatt ttgattatag tttcattaaa caggacatta acccgtttaa
     6301 aagctgccga ggatgtcgga agagagacat ttgtgaataa cttcatacta ttccaatatt
     6361 ataactttcc gctttctata tatttataag aataacgaat catttatacc atcaagggaa
     6421 tttgttctta aattaaaaaa acaattcccc atctgaatat tataaaatta gaactctcaa
     6481 aatctgtcaa gaaataactt atctaaaaga ggtgtacaat attctaacct attgatttat
     6541 agataataat gcaattaaaa agataatgca gaactaagtt agttgcaaaa aaaattaaag
     6601 cttacgcttt tggattattg ggtaaataaa tttctctatt tctatttcgt tacctgagta
     6661 tcattgtctt ctttatagaa gtaaatctct ggttataacg aactactaaa taatattcac
     6721 cagagggtgt gtaagagcca tcatccccaa ttctatccca accaatacgc agataatttt
     6781 ttatcatacc actataaagt gttttaatta ccttaccact gccagtttca atacttattt
     6841 gaacctcaac aggactacta aaacttacgg ttatattggt ataattatcc attggattgg
     6901 gatcaatcac tatttctgta taatctttca tctcttttgc tgacaacata gtcagcatta
     6961 aaagacccat tgctatgaca aataatatct ttttcatttt ttcactctcc tctaatcttc
     7021 acaaatataa tatatgcata tttcattccg aaacaaattt attaacagat tctgccagaa
     7081 tgatagcacc caagacacct gctttatttc ctgccaaagc tttttcaatt ctggtatgtt
     7141 cagcatttat cgcgggcata aaatctataa tttgtttttg aagttttttc caatcataca
     7201 gagaaccttc cattgcacct cctcctatga caataatttc ggggtcaaga agcacactaa
     7261 ggttggtaat gccctgtgcc agaatatata atccttcctc taaaatttgc ttagccttag
     7321 agtctattgc tgttaagtag agcaaatctt taacatttaa ggaagattct gttttggaga
     7381 aaggtatttc cagtagactt attcttcttt tcagaccgtc cacagaggaa tatgcttcaa
     7441 cacaaccttt tctaccacaa gaacataatt ctccaccagg tattatacat atatgtccca
     7501 gttccatggc ataaccatga gaaccgtgaa atattctatc tttttgaaca aatccacatc
     7561 caataccact tccaatagtt attcctacaa tatcacattt aaaatccctg agccaagctt
     7621 cagcaaaaca catcaaatta gcgtcattat cccaataaac gggaatcttt atattttcag
     7681 gaattagatt ctctatattt aaatttacca gaaaaggtag attaggattt actccaacta
     7741 tttgatgaat attctggtct attgttcctg gagtcccaat accaatagct ttgatttttt
     7801 gccagatagg attatcagaa gcaaaattta gaatctcgga gataatattt ttcaagaatg
     7861 gaagggtttt ggtgctgagt tcttttttat tgaaatactg taaacctact ttactattcc
     7921 cccatccata tttgacactg gatgcgccaa tatctattcc taagaagtat ttatctaatg
     7981 ttgacatact aaagaatggg gtgggtgatg gggatcgaac ccacaacgcc cggaaccaca
     8041 atccggtgct ctgccagttg aactacaccc accgtaaaga taattttaag tatctattaa
     8101 gatggcacgc cagaagggat tcgaaccccc gacccgcagc ttagaaggct gctgctctat
     8161 cctgctgagc tactggcgca ttttttggta gcggtgcagg gatttgaacc ccggacctgc
     8221 ggattatgat tccgacgctc taaccagctg agctacaccg cccgattata tgttaaagag
     8281 aaaaaacccg gtaattatac cggtgttaaa aaatggtagc gagggaagga ttcgaacctt
     8341 cgaccttcgg gttatgagcc cgacgagcta ccagctgctc tacctcgcgt ctaaaatatc
     8401 aaaaaatttt aatctaagat ttctgtcaag ttttttgaca aatt
//
LOCUS       DAORZF010000055         8202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_6310_length_8202_cov_375.197619, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000055 DAORZF010000000
VERSION     DAORZF010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 8202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8202
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6310_length_8202_cov_375.197619"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1409)
                     /locus_tag="PK015_06355"
     CDS             complement(<1..1409)
                     /locus_tag="PK015_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HOL34122.1"
                     /translation="MEEQNNRIPRTLPVLHMNNVVMFPYLLMPLVVSDEESRQVIDYA
                     LSQDKTMAFFLDKDKSSPNDLGLHEIGCAVTILRMLRNHDGSISLLLQGTSRIRLQRA
                     VQREPFIIVDVESIPEPADDDTEVRAYKSIAIELLEKIATENNLISPEIIPGLNNIKQ
                     AGRVADIIAGNLDLPLEDRQAILEAIDLKQRFKYLNNCMAEMIRKMRLENTIRNNIQL
                     EMSEDQRRYYLREQLDAIRKELGESDEVSKEILKWNELIEKNNLPDYVKDVAYEELER
                     LATMQPAASEYAVVRNYLEWIVNLPWRTYSKDRLDLNKIEQILQKDHYGLQKPKERIL
                     EYIAVKKLKGHLKGPILCFVGPPGTGKTSIGQSVARALNRKFIRMSLGGIHDEAEIRG
                     HRRTYIGAMPGKIIMEIKRCGTANPVFMLDEIDKLGRDFRGDPASALLEVLDPEQNHS
                     FVDNYINLPFDLSEVMFITT"
     gene            complement(1515..2093)
                     /locus_tag="PK015_06360"
     CDS             complement(1515..2093)
                     /locus_tag="PK015_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34123.1"
                     /translation="MHFLLSLLFVVVNIALAMFLAYLSKWVLFNPKPKKFLGWHIPLT
                     PGFIVSKRNQIFAKIYDTLQDYLNQAENPDLREGYLYEWEEQVRLSALEQVSFIDKVP
                     LLPAGCKSKIKNAMANSAKNTVSFILRRTVPQLMEKFQLEHKLEQLDAKISSEVIYGY
                     FRQYIYKPLLIAAAVAGLLIGVINMVLYLILV"
     gene            complement(2116..3354)
                     /locus_tag="PK015_06365"
     CDS             complement(2116..3354)
                     /locus_tag="PK015_06365"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HOL34124.1"
                     /translation="MKSGNMQDTKDKIEFCPIEEAINIIAGGGMLIVVDDENRENEGD
                     LLMAAEAVTPAQVNFMITHGKGLLCVPMEAEDLQRLHIPLMTKYSTDRQGTKFTISVD
                     AVEGTTTGISATERARTIRTLANPDSKPEDFLRPGHIFPLLAEKGGVLKRAGHTEAAV
                     DLAKMAGLAPVGAICEIIREDGEMARLDDLIPFAKKFGLRIVTIADLINYRRHKEKLV
                     RCESRAKLPTFYGDFDILTYVSTVSDEYHIALVMGEIKKDEPTLVRVHSECLTGDALF
                     SLRCDCGEQLKKAFELISEEGKGVILYMRQEGRGIGLLNKLRAYHLQDNGMDTVEANL
                     NLGFPADLRDYGIGAQILKDLGLKKIRLMTNNPKKIVGLQGYGLEIVERVPLEIPAKD
                     TNLEYLQTKKNKLGHILNQV"
     gene            complement(3341..4372)
                     /locus_tag="PK015_06370"
     CDS             complement(3341..4372)
                     /locus_tag="PK015_06370"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOL34125.1"
                     /translation="MSDKVQVIYDCEDSVRLDKFLAGLNIQELYSRTFIEHLIEEDRI
                     IVNKIPVKKSYLLKKGDEIEISIPELPPTEIVPQNIPLEVVYEDEDLAIINKVAGMIV
                     HPGYGIADHTLVNAMVYRWGNQLSSGRDINRPGIVHRLDRGTSGLMIIAKNDPTQSAL
                     CEMLSRREIKKTYIAITCGIPHPSEGIIDTNISRSISNPRKMCVSQQGRRAITRYKVI
                     QYYSFFSLVKIGLETGRMHQIRVHFAHLKNPILGDLLYNSHKQVKTLVPENMKHKVSE
                     LLTEHLLRQALHSWRLEFTHPISGKHLDIKAEPPEDFQYTFNWLEQYFSLDAKVKDLR
                     TILEENEEW"
     gene            complement(4365..5105)
                     /locus_tag="PK015_06375"
     CDS             complement(4365..5105)
                     /locus_tag="PK015_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34126.1"
                     /translation="MKRSLSSLYHLIIAAILVLILLETAILIVISTNAKDLTNLINDI
                     QTAVIVFALILFIYCIVVYNLIPANIKKFNQHMVKLINEIAHGNYNANIDKAIEDYSF
                     DPEKLSLLSSLKNMLRSINGFDQAKEWKIYEQDQRIKQLINLIPQGVLIALSNGDISY
                     YNNSLRQRYPLLSDIRNINDLNFKNDFDRRIFQKIADALRYGDNLYDCKIPDQDYKRL
                     AIIKGSMVRNLKGEPTGGVFLLSIEEHV"
     gene            complement(5102..5812)
                     /locus_tag="PK015_06380"
     CDS             complement(5102..5812)
                     /locus_tag="PK015_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOL34127.1"
                     /translation="MASYAVIGLGRFGMTVATILAETGMEVLAIDKDQSLVDAISGKV
                     TQAICMNSTDEDALRNLNLDEMDAVILGIGSNIEESVLTAAILKKIGVGMIHAKVENE
                     LHGRILELIGVQRVLLPEKIVGTQLAKTLSSRNILEYLTLTTGLGVMELLAPPEFVGK
                     TLQELALPTTRKINVIAIKYNYLTVTDEGKNLIEQRMNEMPGANDVVNEGDILVLMGK
                     IKDLDNLVDDLAQRKELL"
     gene            complement(5822..>6700)
                     /locus_tag="PK015_06385"
     CDS             complement(5822..>6700)
                     /locus_tag="PK015_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOL34128.1"
                     /translation="PIREAIYYSIFHSISAFCNAGFSPFDSNLMMYSDSIIISLGIPL
                     LIFFGGLGFTVILDLYRYFFKPQKVRKLSLHSKIVLTTTAILIIVGLIAFFILEYNNT
                     MKGFSFKHRFLASLFQSVTPRTAGFNTIDNGLLNKGSVLMTLLLMFIGASPGSTGGGI
                     KTTTLAVLALTIISMLKGRKDISVFKRRIPKDNFREASGLVFLAATVIFVIVMILMMV
                     EPFSFEDIIFESISAFGTVGLSRGITPSLSSLGKILITLLMYIGRVGPLTLIYAFAKR
                     RNHSNINYAEESIAIG"
     assembly_gap    6701..6800
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6801..7291)
                     /locus_tag="PK015_06390"
     CDS             complement(<6801..7291)
                     /locus_tag="PK015_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Trk"
                     /protein_id="HOL34129.1"
                     /translation="MNKISQQVLKAIEILAYLVALWSLLVLFLEPVISYYADFVKVEN
                     FTAWANLLLLALAIINRLLLKEGKGINGATIFDIIMLVVGLVILVYNAKFVIFLLLIR
                     QTYYFLQFILFRFSEGKIYLWLSSNPPITLLLSFLLVIFLGTILLMLPVSSTYNRVTP
                     FVDA"
     gene            complement(7292..7930)
                     /locus_tag="PK015_06395"
     CDS             complement(7292..7930)
                     /locus_tag="PK015_06395"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HOL34130.1"
                     /translation="MQCFVFLGIQGSGKGTQAELLSERINFQHINIGDLLREQVMKRT
                     ELGLKVQDIIGRGDLVPDALVFEIVESSLNPDRKGIVFDGFPRTLVQAKYLVEHFEVL
                     QVFFLELAEDEAIARLSSRRVCPACGANYNILTNKPNKDNFCDVCGTELIIRQDDQPE
                     VISRRVKEFYEQTLALKEFFAKRGILSVIDASEHIEEIAAKIADIAERLLKK"
     gene            complement(7955..>8202)
                     /locus_tag="PK015_06400"
     CDS             complement(7955..>8202)
                     /locus_tag="PK015_06400"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOL34131.1"
                     /translation="AKEAMMKALGTGWNEKVNWLDIEVINERQGKPILKIGGKTLQLM
                     QQMGVRQTHLSLSHERDYALAFVVLEGEKLDNTDNDV"
BASE COUNT         2425 a         1689 c         1393 g         2595 t
ORIGIN      
        1 gtggtaataa acatcacttc tgaaaggtca aaaggaagat tgatgtaatt atctacaaag
       61 ctatgattct gttcagggtc cagaacttcc aataaagcag aagcgggatc acctctgaaa
      121 tcacgtccca gtttatcaat ctcatccaac ataaacactg gattagcagt tccacagcgt
      181 ttaatttcca taataatctt acctggcatt gcgccaatat aggttctgcg atggcctctg
      241 atttcggctt catcatgtat accacccaga gacatacgga tgaatttacg atttaatgct
      301 cgggcaactg actgtccgat agaggtttta cctgttcctg gaggtccaac aaagcagagg
      361 ataggtcctt tcaagtgtcc ttttagcttc ttgactgcaa tgtattccag aatccgttct
      421 ttgggctttt gcaaaccata gtggtctttt tgcaggattt gctcaatctt attgaggtcc
      481 agtcggtctt tactataagt gcgccaggga agattaacta tccattccag ataattccga
      541 accactgcat attcacttgc agcaggttgc atcgtagcaa gacgttctaa ttcctcatat
      601 gccacatctt tcacatagtc aggaaggtta ttcttttcaa ttaattcatt ccatttcagg
      661 atttctttgc taacctcatc gctttcgcct aattcttttc ggatagcgtc cagttgctct
      721 cttaaataat aacgacgctg gtcttcactc atttccagct gaatgttatt acggatagta
      781 ttttccagac gcatcttacg aatcatttca gccatacagt tgttcaggta tttgaatctt
      841 tgctttaagt ctatcgcttc caggatagct tgacggtctt ccaacggtaa atccagattt
      901 cctgcaatga tatcagccac tcttccagct tgcttaatgt tattaagtcc tggaattatt
      961 tctgggctga tgaggttatt ttctgtggca attttttcca atagttctat tgcaatactc
     1021 ttataagcac gcacttcagt atcatcatca gcaggttcgg gaatactttc cacatcaaca
     1081 atgataaaag gttctctttg gacagcacgc tgtaaacgga tacgtgaagt tccttgcagt
     1141 aagaggctaa tagaaccatc atgattgcgc agcattctta agatagttac agcacaacca
     1201 atttcgtgaa gtcccaggtc attgggactg gatttatctt tatccaggaa aaacgccata
     1261 gttttatcct gcgagagagc atagtcaatc acttgccggg actcttcatc agaaaccaca
     1321 agaggcatta gcaaataagg gaacattacc acattgttca tatgcagcac tggaagagtt
     1381 ctcggtattc tgttattttg ttcttccata taattatctc ctataattat aatttttaga
     1441 tttgaaaatt aaggtatagc cttttagata taatactcaa aaaatagcca tacccttttc
     1501 aataattaca tacctcatac taaaatgagg taaagcacca tatttataac tcctatcagc
     1561 aaacctgcca cagcggcagc aataagtaaa ggtttataga tatactggcg gaaatatcca
     1621 taaatcactt cactactaat ttttgcgtcc agttgctcca gcttatgttc cagctggaat
     1681 ttttccatca gttgtggaac ggttctccgt aagataaatg aaactgtatt cttggcagaa
     1741 ttagccattg cattttttat cttactcttg caacccgcag gtaaaagagg cactttatca
     1801 atgaaagaaa cttgttccag tgcgcttaat cttacctgct cttcccattc ataaagatat
     1861 ccctcgcgta aatcaggatt ttcagcctga tttaaataat cctgcagggt atcatatatc
     1921 ttagcaaata tctggtttct tttactgaca atgaatcctg gtgttaatgg gatatgccaa
     1981 cctaagaatt ttttaggttt gggattaaac agtacccatt tagaaagata agccagaaac
     2041 attgccaggg caatgtttac taccacgaat aggagtgata ggagaaagtg cataaaaaac
     2101 tccttttatt ataatttaaa cctgattgag aatatgtcca agtttatttt tcttagtttg
     2161 aagatattcc agattggtat ctttggcagg tatttccaga gggactcttt caactatttc
     2221 caagccataa ccctgtaagc ctacaatctt tttcggatta ttggtcatta acctgatttt
     2281 tttaagtccg agatctttaa ggatttgagc gcctatacca taatctcgta agtctgctgg
     2341 aaagccaaga ttgaggttcg cttccaccgt gtccatccca ttatcctgta aatgataagc
     2401 acggagttta tttaataaac ctattcctct tccttcctga cgcatatata atattacccc
     2461 ttttccttct tcagaaataa gctcaaatgc tttcttcagt tgttctccac aatcacaacg
     2521 aagggaaaaa agagcatcac cagtgagaca ttcagaatgc acgcgcacca aagtgggttc
     2581 atctttctta atttcaccca taaccagggc aatatgatat tcatcagaaa cggtgctaac
     2641 ataggtaagg atatcaaaat ctccataaaa agtaggaagt ttagcacgtg attcacatct
     2701 gacgagtttt tctttatgcc tgcggtaatt aatcaggtct gctatagtta ctattctaag
     2761 tccgaatttt ttagcaaagg gaattaaatc atccagcctt gccatttctc catcttcacg
     2821 gataatttca caaatagcac ccacaggtgc aagtccagcc atttttgcca aatctactgc
     2881 agcttccgta tgaccagctc tttttaagac tccacccttt tctgcaagta gggggaagat
     2941 atgacctgga cggagaaaat cttctggttt actatcagga tttgccagag tacggatggt
     3001 tcttgctctt tccgtagcag aaatccctgt agttgtccct tctactgcat ctacagaaat
     3061 agtgaacttc gtaccctgac ggtcggtgct atatttcgtc attaaaggga tatgtaatct
     3121 ttggaggtct tctgcttcca taggcacaca aagtaaacct tttccgtgag taatcataaa
     3181 gtttacttga gctggtgtga ctgcttcagc agccattaaa aggtctcctt cgttttcacg
     3241 gttctcatca tccaccacta ttagcattcc accaccagca atgatattaa ttgcttcttc
     3301 tatggggcaa aattctattt tatctttagt gtcttgcata ttaccactct tcattttctt
     3361 ctaatattgt tcttaaatct tttactttgg catctaagct gaaatattgt tccagccagt
     3421 taaaagtgta ttgaaaatct tctggtggct cagctttaat gtccagatgt tttccagaaa
     3481 tcggatgagt aaattctaac ctccaggaat gcaatgcctg tctcaataga tgttcggtta
     3541 ataattcgga aactttatgt ttcatatttt ctggaactaa agttttaacc tgtttatgac
     3601 tgttatacaa taaatcaccg aggataggat ttttgagatg agcaaaatgc actcttattt
     3661 gatgcatcct tcccgtttca agtcctatct ttaccagaga gaaaaagcta taatactgga
     3721 taactttgta tctggtaata gctctacgac cttgctgact aacacacatc tttcttggat
     3781 tgctgatact cctgctgata ttagtatcta ttattccttc cgaaggatga ggaataccac
     3841 aggtgatagc aatataggtc ttttttattt ctctgcggga aagcatttcg caaagagcac
     3901 tctgagtggg gtcatttttt gctattatca tcaaaccaga agttcctcta tccaatctat
     3961 gaacgattcc tggacgatta atgtctcttc cactggatag ctgattgccc cagcgataga
     4021 ccatagcatt taccagcgtg tgatcagcaa taccatagcc aggatgcaca atcattcctg
     4081 ctactttatt aattattgcc aagtcttcat cttcataaac tacttccaga ggaatgtttt
     4141 gaggtactat ttccgtgggt ggaagttctg ggatagatat ttctatctca tcacctttct
     4201 tcaatagata gctctttttg acgggaattt tatttacaat tatccggtct tcttcaatta
     4261 aatgttctat aaaggtgcgg ctataaagtt cctgtatatt tagtcctgcc aggaacttat
     4321 ccagtcgcac agaatcttca cagtcgtaaa taacctgaac tttatcagac atgttcctca
     4381 atagatagga ggaagactcc tccagttggt tcacctttca aattgcgaac catagaacct
     4441 tttattattg ctaatctttt atagtcttgg tctggaatct tacagtcata gagattatct
     4501 ccataacgca aggcatcagc tattttttga aatatcctgc gatcaaaatc atttttaaag
     4561 ttcaaatcat taatattccg aatatcactc agtaatggat atctctgcct taaggaattg
     4621 ttataatagc taatatcccc attagaaaga gcaataagca ctccctgggg gataagattt
     4681 atcagttgct tgattctctg gtcctgttca taaatcttcc attcttttgc ctggtcaaaa
     4741 ccattaatgc tgcgcaacat attctttaga ctgcttaaaa gtgatagttt ttccgggtca
     4801 aaggaatagt cttcaattgc cttatctata ttggcattgt agtttccatg tgcaatttca
     4861 ttgattagct taaccatatg ttgattaaat ttcttgatat tagcaggaat aagattgtaa
     4921 accacaatac aatagataaa caggattaaa gcaaatacaa ttactgcagt ttgaatatcg
     4981 tttatgaggt tagttaaatc ttttgcattg gtagaaataa ctatcaagat agctgtttcc
     5041 aaaagaatca ggactaaaat ggctgctata atcaggtgat ataatgaaga aagagaccgt
     5101 ttcataacaa ctccttcctt tgggctaaat catccaccag gttatccagg tccttaattt
     5161 tacccatcaa taccaggata tctccttcat ttacgacatc attagcacca ggcatttcat
     5221 tcattctttg ttctatcaag ttttttcctt catccgttac ggttagataa ttatatttta
     5281 tagcgattac attaatctta cgagtggtgg gaagagcaag ttcttgcaag gttttcccta
     5341 caaattctgg aggagctaaa agttccataa ctcccagtcc agtagtaaga gttaggtatt
     5401 ccagtatatt tctggagctt agagtcttag ccagttgagt tcccacaatt ttttctggaa
     5461 gcagcactct ctgcacccca attaattcca ggattcttcc gtgtaattca ttttctacct
     5521 tagcatgtat cattcccact ccaatctttt tcaggatagc agcagttagg acagattctt
     5581 ctatattact gccaattccc aggattactg catccatttc atccagatta agattgcgta
     5641 aggcatcttc atcggtagaa ttcatacata tagcttgggt gactttacca ctgatagcat
     5701 caaccagtga ctggtcttta tcaatagcta aaacttccat tccagtttct gccagaatgg
     5761 tggctacagt catcccaaat cttccaagtc caataacggc atagcttgcc atatttcctc
     5821 ctcagcctat ggctatggat tcctcagcat aatttatatt gctatgattt cttctttttg
     5881 caaaggcata aatcagagta agaggaccta ctctgcctat gtacattaat aaagtaatta
     5941 aaatcttacc cagtgatgat aaagagggag ttattcctcg agacagacca acagttccga
     6001 atgctgatat tgattcaaaa ataatatctt caaagctgaa aggctctacc atcattagaa
     6061 tcatcacaat tacaaagata acagtggcag ctaaaaatac cagaccactt gcttccctga
     6121 aattgtcttt gggtatacgg cgtttaaata cactgatatc ttttcttcct ttaagcatag
     6181 aaataatggt taatgccagt acagccaaag tggtggtctt gattccacct ccagtagaac
     6241 cgggagatgc accaataaac atcagtagca gagtcatcaa aaccgaacct ttatttaaaa
     6301 gtccattatc aatggtgttg aatcctgcgg tacgtggagt tacagattgg aataaggaag
     6361 caagaaagcg atgcttaaag gaaaatccct tcatggtatt attatattcc agaataaaga
     6421 aagctatcaa tccgacaatt attaaaatag cggtggtagt aagcacaatt tttgaatgta
     6481 gacttagttt tcgcaccttc tgaggtttaa agaaataacg atacaggtcc aggataaccg
     6541 taaatccgag accaccaaaa aatatcaata atggaattcc caggctaatt attatgctat
     6601 cagaatacat catcaggttg ctatcaaagg gagaaaagcc agcattacag aaagctgata
     6661 tggaatggaa aatggaataa tagatagcct cacggatggg nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn gcatccacaa aaggtgtgac tctattataa gtggaagaaa
     6841 caggcaacat caaaagaata gttcccagaa atatcactaa taaaaaactc aatagtaagg
     6901 ttataggagg attagaggat aaccagagat atatctttcc ctcggaaaaa cggaaaagta
     6961 taaactggag aaagtaatag gtctgacgaa tgagtaataa gaaaatcaca aactttgcat
     7021 tatagaccag tattactaac cctactacta acataattat atcaaagata gtagctccgt
     7081 taataccttt tccttctttt agaagtaaac gatttataat tgccagagca agcagcaaca
     7141 agttagccca ggctgtgaag ttttcaacct taacaaaatc cgcataataa ctgataactg
     7201 gttctaagaa taggaccagc aagctccaga gagccaccag ataagctaag atttctatgg
     7261 cttttagaac ctgttgcgat atcttattca tttatttttt cagcaatctc tcagcaatat
     7321 cagcaatttt ggctgctatt tcttctatat gttcactggc gtctataacc gaaagtattc
     7381 ctcgtttagc gaagaattct ttcagagcca gagtttgttc ataaaattct tttacccttc
     7441 gggagataac ctctggttgg tcatcctgac ggataattaa ttctgttccg caaacatcgc
     7501 agaagttatc tttattaggt ttattagtca atatattgta atttgcacca caagcaggac
     7561 aaactcttcg cgaagataaa cgtgcgatag cttcatcttc tgccagttct aaaaagaaga
     7621 cctgaagcac ttcaaaatgt tcaaccagat acttagcttg caccaaagtg cgaggaaatc
     7681 catcaaatac tatccctttt cggtctggat ttaaagaact ttctactatc tcaaaaacca
     7741 gcgcatcagg gactaaatct cctcgtccaa ttatatcctg gacctttaag ccaagctctg
     7801 tccttttcat cacctgttct cttaataaat ctccgatatt tatatgctga aaatttattc
     7861 tttcactcaa tagttctgcc tgagtgcctt ttccactgcc ttgaattccc aagaatacga
     7921 aacattgcat ttttaccacc ttatttttga gccattatac atcattatct gtattgtcaa
     7981 gtttctcacc ttccagaaca acaaaggcaa gggcataatc tcgctcatga gaaagggaca
     8041 gatgagtttg acgcactccc atctgttgca tcaattgtag cgtctttcct cctatcttca
     8101 atatcggttt gccttgcctt tcatttataa cttcaatatc caaccagtta actttttcat
     8161 tccagccagt acccagagcc ttcatcatag cctctttagc ag
//
LOCUS       DAORZF010000056         7289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_7227_length_7289_cov_360.435444, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000056 DAORZF010000000
VERSION     DAORZF010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 7289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7289
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7227_length_7289_cov_360.435444"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..995
                     /locus_tag="PK015_06405"
     CDS             48..995
                     /locus_tag="PK015_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34132.1"
                     /translation="MIKVKWENNQIPEDIKLPSGWVFFCFKKEDLALWCGFTPNISQR
                     LHNISRKAEQDESFRTASLAAEYIEIEPYSRSVDALFHYKVFVQEYNPPFQDVTAPWK
                     EYVYLALDAYHFPFVSIQSDTNGDWTYAGPWRSRFFLMDIMDSISRILKLPFCETSYY
                     PCDKLDKGICRGYCEALDEEGEVEDVEELNKLESLLWEAYLHPDNSILEMLTKEKEKY
                     FNELEFEKEDLITSEVENFAKYRDWLKFLYLTKTLSYQTEDFGVENGLLSWCKFKDKI
                     YKFPVEPINYRPNERLAINLNKTDEARLLYEYYIQNYKG"
     gene            999..1886
                     /locus_tag="PK015_06410"
     CDS             999..1886
                     /locus_tag="PK015_06410"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOL34133.1"
                     /translation="MYEILSKLPIVKQSRQGKVRDMYDLGETVLMVTSDRISAFDVVF
                     PDPIPGKGIILNQIAAYVFKTTSNIVPNHFISDKTEDYPEEFQPYKDYLEGRSMLVQK
                     LRMIPVECIVRGYISGSAWNEYSHSGTVGGEVMPSGLKENQKLPQPIFTPSVKITEGH
                     DINISYQEMLNHMDKFIAEFLRDKSLALYDYGYNFLLPKGIILADTKFEFGTKEGEIL
                     LADEALTPDSSRFWDLEAYNSGKGPVSFDKQIVRDYLESIGWNKKPPAPHLPEEIIDK
                     ALAKYRQIRDIFLGEQSKQ"
     gene            1883..2266
                     /locus_tag="PK015_06415"
     CDS             1883..2266
                     /locus_tag="PK015_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOL34134.1"
                     /translation="MKTICIVDDEEDLVENLKLQLEQIKPEWKIIGFSNGMDALKEII
                     AGNVDLVITDIAMPDMDGYELFWRIKDHNPQMPVIMMTGFGYDPNHVLVRAKSDGLQE
                     ILYKPFELDKLIELIDKHLKPNKKN"
     gene            2296..3330
                     /gene="lpxK"
                     /locus_tag="PK015_06420"
     CDS             2296..3330
                     /gene="lpxK"
                     /locus_tag="PK015_06420"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOL34135.1"
                     /translation="MQKTWLSNLMTPLGKLYEIYQTQRRKKLNSQAWHPDCFVISIGN
                     IMSGGSGKTPFCLYLAELLKKSGIKIGVSHRGYKGTLEDTPTVISDGKQIFYKAMTCG
                     DEAYLLASRLPEIPVVVGKQRCAAVSLLLAAFPDTEIVILDDAFQHLSIARDLDFICF
                     DAKWGIGNGRVIPAGYLREPVKNIPLSSIIIINHKEGEEEVPLEKKWWSSFPQTSFHC
                     WIRGKGYVDTRDNLYTAKDIKGKRCILVSGIAHPESFEHNIEKDGISWLHHFRYPDHY
                     AYSDIKEINKLSSLCSKYQIDYILCTEKDLVKLQIYAPLKNKLRALRLQTECQDEETL
                     LGLIKERMGY"
     gene            complement(3332..4075)
                     /locus_tag="PK015_06425"
     CDS             complement(3332..4075)
                     /locus_tag="PK015_06425"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOL34136.1"
                     /translation="MPSIFYPSLDANMKPGEQIRLAGEEFHHWAYVAHRRIKETVILN
                     SGKGIMAKAEAVKIEPASALLELTEIIDCPYPDYPFAIAFALLKNRNDELIVEKCTEL
                     GVSALFPLITDLSVRKPSANTLSRFQKIAISAIKQCDNPWLPEIFSPQDLRSALANIK
                     AQSYNPILCSERKPEQWMFDLSAEQAGKPCFMIGAEGGWSTEEFNLMQDLPEICLAHQ
                     ITRAETAAIVASAQWLALAQRLKINTKAS"
     gene            complement(4088..5245)
                     /gene="dnaJ"
                     /locus_tag="PK015_06430"
     CDS             complement(4088..5245)
                     /gene="dnaJ"
                     /locus_tag="PK015_06430"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOL34137.1"
                     /translation="MAKRDYYEVLGVSRDASEDEIKKAYRKLAMKYHPDKNPNNKEAE
                     EKFKEASEAYEVLSDKEKRQLYDQYGHAGIDQQFGNGGFSWNDFSHFDDISDLFGGGF
                     SSIFDTLFGGGFSSRSRESQRYSNQGENIQIDLALTLKEIALGTEKKVRITVKDICDQ
                     CRGTGSADGKAETCPQCHGTGQVRTVRQSLFGQMQSISECPSCRGEGKIIRNKCPKCM
                     GEGRISKNKEINVKIPAGVEENQVIRLRGQGNVGPRNGSYGDILVVIHEKPDELFERD
                     GNNIILELPITVTQAVLGDEVIVPTLTGTAKMKIPPGTQSGRIFRLRGQGIKGLNSYS
                     RGDLLVKIKVVVPTKLSREEMELYNRLKEFDKKRELKPGKSFKEKLRGFFF"
     gene            complement(5320..7278)
                     /gene="dnaK"
                     /locus_tag="PK015_06435"
     CDS             complement(5320..7278)
                     /gene="dnaK"
                     /locus_tag="PK015_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOL34138.1"
                     /translation="MGKIIGIDLGTTNSCVAVVEGGKPVVITNAEGSRTTPSVVAFTK
                     DGQRLVGQLAKRQAITNPKNTVYSIKRFMGRHFSEVQNEIKQVPFTVKEGLKGQAVVH
                     IEVENRDYTPQEISAMILTKMKETAEAYLGQKVTEAVITVPAYFNDSQRQATKDAGTI
                     AGLDVKRIINEPTAAALAYGMDTKKEEKVAVYDLGGGTFDISILDISQGVVEVLSTNG
                     DTHLGGDDFDKRIVDWILDRFKKEEGIDLSKDPMAMQRLREAAEKAKVELSGTPTTNI
                     NLPFITADATGPKHLNYDLTRAEFNRLTSDLVERTLGPCKQALSDAKLTVDDIQEVLM
                     VGGSTRIPAVQEAVEKFFGKKLNHSLNPDEVVAIGAAIQGAILSGDEKVSDVLLLDVT
                     PLTLGIETLGGVMTPLIPRNTTIPTKKSQIFSTAADNQTAVTIHVLQGERPMAADNKS
                     LGRFDLVGIPPAPRGVPQIEVTFDIDANGILHVSAKDLATGKEQSIRIDRTGDISKDD
                     IDRMIRDAELHAEEDRKRQEYVTARNELDNLIFSTEKNLNEYGDKLPAEERSELEAEI
                     KHAKDVLEKATDANELNAVKDELLKKAQRLGEIIYSQAQQQRGAGANNADTGTYEQQD
                     YGQSSNEEQGSNQSEGPIDADFEVMDDK"
BASE COUNT         2196 a         1444 c         1336 g         2313 t
ORIGIN      
        1 cagaactttc tgattgacac aaagactttt gccaaaacta tgtccgtatg ataaaagtca
       61 aatgggaaaa taatcaaata ccagaggata ttaagcttcc atctggatgg gtgttttttt
      121 gttttaaaaa agaagacctt gctctctggt gtggttttac accaaatatt agccagcgtt
      181 tgcataatat aagcagaaaa gctgaacagg atgaatcttt tagaacggct tcactggctg
      241 ctgaatatat agagatagag ccttattctc gttcggtaga tgccttattt cattacaaag
      301 tctttgttca ggaatataat cctccttttc aagatgtgac tgcaccctgg aaagaatatg
      361 tctacctcgc tcttgacgca tatcatttcc cttttgtatc catccaatct gacactaatg
      421 gtgattggac ttatgcagga ccctggagaa gtcgtttttt cttgatggat ataatggata
      481 gcatttctcg tatactgaaa cttccctttt gcgaaacttc ttactatcct tgtgacaaat
      541 tggataaagg aatctgtaga ggttattgtg aagctttgga tgaagaaggt gaagtggaag
      601 atgtggaaga attaaataag ttagaatctc tcctctggga agcctatctt catcctgaca
      661 atagtatcct ggaaatgctt actaaggaaa aagaaaaata tttcaatgaa ctggaatttg
      721 aaaaggaaga tcttattacc agcgaagttg aaaactttgc aaaatataga gattggctaa
      781 aatttctcta tctaaccaag actttatcat atcaaacgga agattttggg gtggaaaatg
      841 gtctacttag ctggtgtaaa tttaaggata aaatctataa attcccagta gaaccaataa
      901 attatagacc caacgagaga ttagccatca atttaaataa aaccgatgaa gcaagattac
      961 tctatgaata ttatattcaa aattataaag gataagcaat gtatgagata ttaagtaagt
     1021 taccaattgt aaagcaaagt cgtcagggaa aagtgcgaga tatgtatgat cttggtgaga
     1081 ccgttttaat ggtgaccagt gaccgcattt ctgcttttga tgtagttttt ccagacccta
     1141 ttcctggcaa aggaataata ctcaatcaga ttgctgctta tgtttttaaa acaacttcca
     1201 atattgttcc caatcatttt ataagtgata agacagaaga ttatccagaa gaatttcaac
     1261 cttataaaga ttatctggaa ggtagaagta tgttggtgca aaagctccga atgatacctg
     1321 tagaatgcat agttagagga tacatcagtg gttctgcctg gaatgaatat tcccattcag
     1381 gaactgttgg aggtgaagtt atgccatctg gactgaaaga aaatcaaaaa ctacctcaac
     1441 ctatttttac accttccgtc aaaatcactg agggccacga cataaatatc agttatcaag
     1501 agatgctcaa tcatatggat aaatttattg ctgaattttt gagagataaa tctcttgctc
     1561 tctatgatta cggctacaac tttttactac ccaaaggaat cattttagct gacaccaaat
     1621 ttgagttcgg aaccaaagaa ggggaaatac ttttagctga tgaagcactt acacctgatt
     1681 cttctcgttt ttgggatttg gaagcatata attcgggaaa aggtcccgta agctttgata
     1741 aacagatagt gcgtgattat ctggaaagta ttggctggaa taagaaacct cctgctccgc
     1801 atttaccaga agaaataata gataaggctt tagctaagta tcgtcaaatc cgagatatct
     1861 ttttaggaga gcaatccaaa caatgaaaac tatttgtatt gttgatgatg aagaagattt
     1921 agtagaaaat ttaaaactcc aattggaaca gattaaaccc gagtggaaga taatcggatt
     1981 cagcaatggt atggatgctc tgaaagaaat tatcgctgga aatgttgatt tagtgataac
     2041 tgatattgca atgcccgata tggatggcta tgaacttttc tggaggatta aagaccacaa
     2101 tccccaaatg cccgttataa tgatgactgg ttttggatac gaccctaatc atgttttagt
     2161 aagagcaaaa tctgatggat tgcaggaaat actctataag ccatttgaac ttgataaatt
     2221 gatagaactt atagataaac acttaaaacc taataagaaa aattgataag tccttccttt
     2281 attgataagc atttaatgca aaaaacctgg ctcagcaacc tgatgactcc attaggtaaa
     2341 ttgtatgaaa tctaccaaac tcaaagaaga aaaaaactca attcccaagc ttggcatcct
     2401 gattgttttg ttatcagtat aggcaacata atgagtggtg gtagtggcaa aacccctttt
     2461 tgtctgtatc tggctgaatt attaaagaaa tcagggataa aaattggagt atctcataga
     2521 ggttataaag gaactttaga ggatactcca acggttattt ctgatggaaa acagattttc
     2581 tataaggcaa tgacctgtgg agatgaagcc tatcttcttg cctctcgctt gcctgaaatt
     2641 ccagtggtgg taggaaaaca acgctgtgct gctgtttcac tcttactggc agcatttcca
     2701 gatactgaaa tagttatctt agatgatgct tttcaacatc tcagtattgc acgagacctg
     2761 gattttattt gctttgatgc taaatggggc attggaaatg gaagagtgat tccggcagga
     2821 tatttaagag aaccagtaaa aaatatacct ctttcctcta taattataat caatcataaa
     2881 gaaggtgaag aggaagttcc tctggaaaaa aagtggtggt cttctttccc ccaaacttcc
     2941 tttcattgtt ggataagagg aaaaggttat gtagatacga gggataatct ctatacggct
     3001 aaagatatta aagggaaaag atgcatactg gtaagtggaa tcgctcatcc tgaatccttt
     3061 gaacataata ttgaaaaaga tggaatctcc tggctacacc actttcgtta tccagaccat
     3121 tatgcttatt ctgatattaa agaaataaat aaactttcca gtttatgcag taaatatcag
     3181 atagattata ttctttgtac ggaaaaagac ctggtcaaat tacagattta tgcaccactg
     3241 aagaataaat tacgggcact tcgtctgcaa actgaatgtc aagatgaaga aactttgttg
     3301 ggtctaatta aagaaagaat gggatattaa tttaactggc ttttgtgttt attttcagtc
     3361 tttgagctaa agctaaccat tgcgctgaag caacaatggc agcagtctcc gctctggtaa
     3421 tctgatgagc taaacaaatt tccggtaaat cctgcataag attaaattct tctgtgctcc
     3481 aaccaccctc tgctcctatc ataaaacaag gtttccctgc ctgctcagct gataaatcaa
     3541 acatccattg ctctggtttg cgctctgaac aaagaatagg attataactt tgagctttaa
     3601 tgtttgctaa agcgcttctt aaatcctgcg gagaaaatat ttccggtaac cagggattgt
     3661 cacattgttt aatggctgag atagctattt tttgaaaacg agataaggta tttgcagacg
     3721 gttttcttac agataaatct gtgattaagg ggaaaagagc gctcactcca agttctgtgc
     3781 acttttctac aatcagttca tcattacggt tcttcagtaa ggcaaaagct atggcaaaag
     3841 gatagtctgg atagggacaa tctataatct cagtaagttc taataaagca gaagcaggct
     3901 caattttgac cgcttcagct ttagccataa tccccttacc agaatttaat ataacagttt
     3961 ccttgatacg cctatgagcc acataagccc agtgatgaaa ctcttcgcca gccaagcgaa
     4021 tttgctcacc tggcttcata ttggcatcta aggaaggata aaatattgaa ggcatcttcg
     4081 cttttaatta gaaaaagaag cctctcaatt tctctttaaa gctcttacca ggcttcaatt
     4141 ctcgcttctt atcaaactct tttagacggt tataaagttc catctcttct cgggaaagtt
     4201 tagtgggcac tacaactttg attttgacca acaaatctcc ccgactgtag gaatttagtc
     4261 ctttaatacc ctgtcctctt aggcgaaaga ttctaccact ttgagttcca ggtggaatct
     4321 tcatttttgc tgtgccagtt aaggttggaa ctattacttc atcacccaga acagcttgag
     4381 taacagttat aggaagttcc aggataatgt tatttccatc cctttcaaaa agctcatccg
     4441 gtttttcatg aatgacaacc aggatatcac cataacttcc atttctggga cctacattgc
     4501 cctgacctct aagtcggata acctgatttt cttccactcc tgcaggaatc ttgacattaa
     4561 tttccttatt tttactaatt ctaccctctc ccatacactt gggacattta ttgcggataa
     4621 ttttaccttc tcccctgcaa gaaggacatt ctgatatgga ctgcatctga ccgaagagcg
     4681 attgacgcac ggttcgcacc tgtccagttc cgtgacattg aggacaggtc tccgcttttc
     4741 catcagcact tccagttccc ctgcattggt cacagatatc tttgactgta attcttacct
     4801 ttttctccgt ccctaaagca atctccttaa gggttaaagc taaatctatc tgaatatttt
     4861 caccctgatt ggaatacctt tgactttctc tgctacgaga actaaatcca ccaccaaaga
     4921 gggtatcaaa aatagaactg aaccctccac caaaaaggtc actgatatca tcaaagtggc
     4981 tgaaatcatt ccagctaaat ccgccgttac caaattgctg gtctattcct gcatgaccat
     5041 attggtcata taattgacgt ttttctttgt ctgagagaac ttcataggct tcggaagctt
     5101 ctttgaactt ctcttctgct tctttattat tcggattttt atccggatga tatttcattg
     5161 ccagtttgcg ataagctttt tttatctcat cttcactggc atcccttgag acaccgagca
     5221 cttcatagta atctcttttt gccatctatt taaactccag aaaatatcaa attagtactc
     5281 cgacaggata aaaatcctgc cggagtgcga ttatatctat tatttatcgt ccattacctc
     5341 aaaatcagca tctattggtc cttcggattg attagatcct tgttcttcat tggagctttg
     5401 accataatct tgctgttcat aggttccagt atcagcatta ttagctccag ctcctctttg
     5461 ttgctgagct tgagaataaa tgatttcacc taagcgttga gcctttttta agagttcatc
     5521 tttaactgcg ttaagctcat tagcatcagt tgccttttct aatacatctt tagcatgctt
     5581 gatttcggct tccagttcgc ttctttcctc tgcaggaagt ttatcaccgt attcattcag
     5641 gttcttttct gtgctgaata tcaagttgtc aagctcattt ctggcagtga catattcctg
     5701 acgctttcta tcttcttcag catgcaattc agcatctcta atcatcctat caatatcatc
     5761 tttactgata tcacccgtgc ggtcaatacg gatagattgt tctttacccg tggctaaatc
     5821 cttagctgaa acatgaagaa taccattagc atcaatatca aaggtaactt caatctgggg
     5881 tactcctctg ggagcaggtg gaattcccac gaggtcaaaa cgaccgagag atttattatc
     5941 agctgccatt ggtctttcac cttgcaatac atggatagtg acggcagtct gattatctgc
     6001 tgcagttgag aatatctgac tctttttagt aggaatggtt gtatttctgg gaatgagagg
     6061 agtcataaca ccaccgagag tttcaattcc taaggttaag ggtgttacat ccagcaacag
     6121 cacatcgcta accttctcat ctccagaaag aatagcaccc tgaatagctg cacctatagc
     6181 aactacttca tcaggattca gactgtggtt cagcttttta ccaaagaatt tttcaactgc
     6241 ttcttgaaca gctggtattc ttgtgcttcc accaaccata agaacttctt gaatatcatc
     6301 taccgtcaat tttgcatcac ttagagcttg tttacaagga ccaagagtac gctctactaa
     6361 atcagatgtt agacggttga attccgcacg agttaaatca taatttagat gttttggacc
     6421 agttgcatct gcagtgatga aaggcaaatt tatattagta gttggagttc cagatagttc
     6481 cactttagct ttttctgcag cttcacgtaa acgctgcatt gccatagggt ccttagacaa
     6541 atctattcct tcttcttttt taaatctatc cagaatccaa tctacgattc ttttatcaaa
     6601 atcatctcca cctaaatgag tatctccatt agtggaaaga acttcaacca caccttgaga
     6661 aatatccaga atagagatat caaaggtacc tccacccagg tcatagactg caactttctc
     6721 ttccttttta gtatccattc cgtaagctaa agctgcagca gtaggctcat ttatgattct
     6781 cttaacatcc agacctgcaa tggtgcccgc atctttagtt gcttgacgct gactatcatt
     6841 aaaataggca ggaacggtta tgactgcctc agtcaccttt tgacctaaat aggcttctgc
     6901 agtttccttc atcttagtga gaatcattgc tgaaatctct tgaggggtgt aatctctatt
     6961 ttctacttca atatggacaa ctgcttgccc tttcaagcct tctttaacag tgaaaggtac
     7021 ctgttttatt tcattttgta cttcactaaa atgacggccc ataaaacgtt ttatggaata
     7081 tactgtattt ttaggattag tgattgcttg tcttttggct aattgtccca ctaaacgctg
     7141 accatcctta gtaaacgcta cgacggaagg tgtggtgcga cttccttctg cattggttat
     7201 aacaactggt ttgccgcctt ctacgacagc gacacaagag ttagttgttc ccaaatctat
     7261 accgattatt tttcccattt ttcctcctt
//
LOCUS       DAORZF010000057         7185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_7351_length_7185_cov_369.226367, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000057 DAORZF010000000
VERSION     DAORZF010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 7185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7185
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7351_length_7185_cov_369.226367"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            288..364
                     /locus_tag="PK015_06440"
     tRNA            288..364
                     /locus_tag="PK015_06440"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Arg,seq:acg)
     gene            411..2339
                     /locus_tag="PK015_06445"
     CDS             411..2339
                     /locus_tag="PK015_06445"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOL34139.1"
                     /translation="MDKSTNTKGNWKDLKKIYGIMFRYWPVLVTGLIAMLLYALFSGI
                     SITFVIPLFDYVFNPNKPAALYTTMGQFFDAIGTAWGSYISGIGGIFAIRGLSDLAPF
                     WESLKQIMLQTNSLSLLYAICLFVVITMVLKNVFYYIQRILFVDLRGNTIRDVRNLMF
                     SRYMKQSLEFFNQNRVGDALVRMVNDVEIVSEQFIRSLLEGIRDVVTILVYMRIALLM
                     NSQLFLYSIIVLPAFSLTIGFLGKKIKKYSRRIQAQLSSMFSTVEEALNSMKIVKAFR
                     REESEYKIFSQINKRHLKLWKKAQRYSALNVPLSEMTTLFTGVIVIVIGGRMILAPGS
                     TFSLGDFTAFLFAMFSMLHPLNSLTQIYTDVKKALVSLGRISTVLNTESSVQEAEDAV
                     EKKDFNSEIVFDNVSFSYKGSKPVLHNVSLTIHKGEKIAFVGASGGGKTTLANLFNRM
                     YDVNEGAILIDGIDIRKIKISDLRRLFGVVTQESVLFTRSVRENIAYGSLDEVSDEQI
                     RKAAQVAHAEEFILNFPNQYDEILQTKGANLSGGQKQRLCIARAIVGDPPILIFDEAT
                     SALDTESEKLVQEAIDDATQNRTVIMIAHRLSTIQKADRIVVLEHGRIVGIGTHQELM
                     ENCPRYQKLYNLQYGHST"
     gene            2342..3289
                     /locus_tag="PK015_06450"
     CDS             2342..3289
                     /locus_tag="PK015_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34140.1"
                     /translation="MKISGFSFVRNGIKLYYPVVESIKSILPICDEFVIAVGQGDEDD
                     TTRESIASLNDPKIKIIDTIWEEKYFKKGIINAYQTDIAKEACTGDWLFYLQADEVVH
                     ERYLPVIQKRCEELLDREEVEGLLFRYKHFWGDYQHYQKAHGWYPYEIRIIRNLPQIH
                     SYQSAQSFRWFDYYEGPRQEKGTRKLKVAKVDAEIYHYGWVRPPHLMQNKCKALNSVH
                     WGKAKAENFYQQAPEYFDYGPLDRLAVFTGTHPTVMEDKIKAMDWQDKLQYSGKPNPF
                     REPHKHERFDNKVLSWIEQNLNGGEQIGTFRNYHLLRNI"
     gene            3296..4042
                     /locus_tag="PK015_06455"
     CDS             3296..4042
                     /locus_tag="PK015_06455"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOL34141.1"
                     /translation="MVKISAVMIVKNESENLKRCLPALTWTDEIVVLDTGSEDDSIKV
                     AQSYGAKTSKLEFWEGFGKAKQKAVELAENDWIISLDADELLSEDLQEELKALKEQDF
                     LNCAWRLKRKSFYEGKLIHFCGWQNDRPLRVFNRLKGNFNDYPVHEGVKTEMEKRCCK
                     GILYHYPYKDKETHFQKMRFYGEIGAKELAKKGKKSNPVTALLRAAAKFLRMFILQLG
                     FLDGRAGFLLCTRSAWGVWYKYHYLWKISQ"
     gene            4024..5121
                     /locus_tag="PK015_06460"
     CDS             4024..5121
                     /locus_tag="PK015_06460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOL34142.1"
                     /translation="MENKPIKVLHIDSEKLWRGGQQQAVYLYEGMQKRGIPCSFVCQP
                     QSKLEQYFQNHNLVYKSIPFSGEMDLMAGFRLSKFVRKHNYTILICHSAHSLSWGLIA
                     KAFYPALKIIGVRRVDFPLKQNPFSRWKYTNSKVNKIVAVSQKIKEVLRKDGVSEDKI
                     KVIYSGIDYNKYAQAEEDTNFRKHFNIPPEAILVGTIAAFAGHKDYPTFLNAAALALK
                     NRPDLYFLAVGKGELQKSMQELAKELNIADKVIFTGFQQEVEKILKTLDIFVLASKKE
                     GLGTSILEAMAAGIPVIGTASGGIPELIKDGENGLLVPSSNPEKLKDAILKLANSPEL
                     RMQLAERGKTEAQKFDKEIMVSQYIKLIQEL"
     gene            5118..6152
                     /gene="waaF"
                     /locus_tag="PK015_06465"
     CDS             5118..6152
                     /gene="waaF"
                     /locus_tag="PK015_06465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HOL34143.1"
                     /translation="MKEIERIFFIQTAFFGDVIMSTPIPRALKQLYPNARLDIILIPE
                     TRIIYQDNPYVDNIYLFDKRNPFKRIPSFLRLVHTLRKNHYDLAVSVHISFSSSLLML
                     LSGAKIRLGYPRQKFKNLTIDLPKGVPVVKRGLLLIKALSDREFDYQTELYINPEIQK
                     KVQEYINSNSLNPEKMIAIAPGSVWATKRWLPEYYAEVVKALTEEGYQCVMIGSAEER
                     KLCEEIIKISGVNAYNSAGNFNILGSAELIRNCRLIITNDSAPLHLANAVKTPVLAIF
                     GPTVERFGCFPYRENDRVLQVDLECRPCGKHGHKKCPEGHFRCMKDITVDMVLKNAKE
                     MLNIGDKEVQ"
     gene            6149..>7185
                     /locus_tag="PK015_06470"
     CDS             6149..>7185
                     /locus_tag="PK015_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34144.1"
                     /translation="MKELYLIVGYNDFFGQTRKPWVSIDTSRLVSELENLGYIVHKNE
                     FHEIVNGKVKIENQIVFYTFSHRLNLQQYIRDCLLYLLDCGNTLIPSYELFCCHENKG
                     WQVLLRRKLGIEGLWNAYFSSKRELKDYEIKYPVVLKTITGSNAKGVFLVKDEEDLLK
                     KIKSLEKQPSIAQKLDFVRRKYFRKYKKIPGYPDYDLFKDAQLYQDYITPEIPFVLQE
                     FIPNLTYDYRVIALGEKYFVSKRLTRKNDFRASGAKRFTFDFPVPVKLLDFARELHHK
                     IHSPYLSIDVGENEEGDLYLFEFQVEHFGINAIVLGKGYYVEKEGKWEFVEEKTPFEK
                     DLAYGLDLFLS"
BASE COUNT         2326 a         1115 c         1554 g         2190 t
ORIGIN      
        1 gaaatagaat aaatattaag ttagctaaaa gaataacccc ggtagcaata ccggggtttt
       61 ttataaaagt taatcccatt gtaactttaa tgacctaatg aggcaagaaa ggaggaaaaa
      121 ggattttgtc cacattaaaa atgttctttt aacaagggat aataatcatt taatatgaag
      181 gtaaataggt tttgaatggt aaaaaaacaa agaaaagaaa aataagattg aaacagtaat
      241 ttgtgcttga cagaataata gtgatttcaa gtatagaaaa aatgggtgcg cccatggctc
      301 aattggatag agcatctgac tacggatcag agggttgggg gttcgaatcc ctctgggcgt
      361 gccaagattt tccttgccgg cggaagctgg ctttttttat agtataactt atggataaat
      421 caacaaatac aaaaggcaac tggaaagacc tgaaaaaaat ctatggcata atgtttcgtt
      481 attggcctgt cctggttaca ggtctgatag cgatgctttt gtatgccctt ttttctggta
      541 tcagtataac ttttgtaatt cctctttttg attatgtgtt caatcccaat aaacctgctg
      601 ctctctatac tactatggga cagttttttg atgcaattgg gactgcctgg ggcagttata
      661 taagtgggat agggggaata tttgcgatta gaggattgag tgatttagct cccttctggg
      721 aaagcctgaa gcagattatg ctccagacca attcactgag tttactttat gcaatttgtc
      781 tgttcgttgt gataacgatg gtgctgaaga atgtttttta ttatattcag cgaattcttt
      841 ttgtagattt acgaggaaac acaatccgtg atgtgcggaa cctgatgttt tcacgctata
      901 tgaagcaatc tctggagttt tttaatcaga atagagtggg tgatgcatta gtgcggatgg
      961 tgaatgatgt agagattgta agtgagcaat ttattcgttc cttgctggaa gggattagag
     1021 atgttgtcac cattctggtt tatatgcgga tagcattatt gatgaattcc cagttattcc
     1081 tttatagcat aattgtttta ccagcttttt ccttaaccat tggcttttta ggcaagaaga
     1141 taaagaaata ttccagaagg attcaggcgc aactctcttc aatgttttcc acagtggaag
     1201 aagcgctgaa cagtatgaag atagtgaaag catttcgtag ggaggaaagt gaatacaaaa
     1261 tctttagtca gataaataaa cgacatctaa aactctggaa gaaagcacag cgatatagtg
     1321 cattgaatgt tcctctctct gagatgacta ctttatttac tggtgtgatt gtaattgtga
     1381 taggtggcag gatgattctt gctcctggct caactttctc tttaggagat ttcaccgctt
     1441 ttctttttgc tatgttttct atgttgcatc cgcttaattc tctcactcaa atatataccg
     1501 atgttaaaaa ggcattagtt tcgctgggta gaatttctac cgtgctgaat acagaatcat
     1561 ctgtgcaaga agctgaagat gcagttgaga aaaaggattt caattcagag attgtttttg
     1621 ataatgtaag ttttagctat aagggctcca aacctgttct acataatgtt tctttaacca
     1681 ttcataaggg agaaaaaata gcttttgtag gtgcaagtgg aggagggaaa accactctgg
     1741 caaatctttt taaccggatg tatgatgtca atgaaggtgc tattcttatt gatggcatag
     1801 atatacgaaa gataaagata tcagatttgc gaaggctttt tggagtagta actcaagaaa
     1861 gtgtgctctt tacaaggtcc gtaagagaaa atattgctta tggttcgctg gacgaggtga
     1921 gtgatgaaca aatcaggaag gcagctcaag tagctcatgc ggaagagttt atcctgaatt
     1981 tccctaatca gtatgatgag attttacaga ctaagggggc aaatctttcc gggggacaaa
     2041 aacagcgctt atgtattgcc agagctattg tgggagaccc acctatttta atttttgacg
     2101 aagcaaccag tgctctggat actgaaagtg aaaaactggt gcaagaagcc attgatgatg
     2161 caacgcagaa cagaaccgtg attatgatag ctcatcgtct ttctaccatt cagaaagcgg
     2221 atagaatcgt agttctggaa cacggtagaa ttgtgggaat tggcactcat caagaattga
     2281 tggaaaactg ccctcgctat cagaaacttt ataatctcca gtatggtcat agcacttaat
     2341 tatgaagata agtggatttt cctttgttag gaatggcata aaactctatt atccggtggt
     2401 agaatccatt aaatctatac tgccaatctg tgatgaattt gtgatagcag taggtcaagg
     2461 agatgaagat gataccacgc gtgaatccat agcttctctt aatgacccca agataaaaat
     2521 catagatacc atctgggaag aaaaatattt taaaaaaggg attattaatg cttatcaaac
     2581 cgatatagca aaagaagctt gcactggtga ctggcttttt tatcttcagg ctgatgaagt
     2641 ggttcatgaa agatatctac cagtcataca gaaaagatgt gaagagctgt tagataggga
     2701 agaagtggag ggtctacttt ttcgctataa acatttttgg ggtgattatc agcattatca
     2761 aaaagctcat ggctggtatc cttatgaaat caggataatt cgcaatctac ctcaaatcca
     2821 ttcctatcag agtgctcaat cttttcgctg gtttgattat tatgagggtc cacgacagga
     2881 aaagggaacc cgaaagctga aagtagccaa agtagatgcg gaaatatatc attatggttg
     2941 ggtgcgacct ccacatttga tgcagaataa atgcaaagct ttaaatagtg tgcattgggg
     3001 caaagctaaa gcggaaaact tctatcagca agcaccagaa tactttgatt atggacctct
     3061 ggaccgtctg gcagttttta ccggaactca tccaacagtg atggaagata aaattaaagc
     3121 tatggattgg caggataaat tgcagtattc tggaaaacct aatcctttca gagagccgca
     3181 taagcatgaa agatttgata ataaagtgct cagctggata gaacaaaatc tcaatggtgg
     3241 agagcagata gggactttta gaaattatca tctactaaga aatatataag tcccaatggt
     3301 aaaaatatct gccgtaatga ttgtgaaaaa tgagagtgaa aatctgaaga gatgtcttcc
     3361 tgcacttact tggacagatg agatagtggt tctggatact ggttccgaag atgacagcat
     3421 aaaagtagct caatcttatg gcgctaaaac ctcaaaactt gagttttggg aaggttttgg
     3481 taaagctaag caaaaagcgg tggaacttgc agaaaatgat tggattattt ccctggatgc
     3541 agatgaactc ttatccgaag atttgcaaga ggaattgaaa gctttgaaag agcaggattt
     3601 tctgaattgt gcctggcgct tgaagaggaa atctttctat gaaggaaaac ttattcattt
     3661 ttgtggttgg caaaatgacc gtcctctaag agtttttaat cgtttaaaag gcaattttaa
     3721 tgattatccc gtgcatgaag gtgttaaaac tgagatggag aagcgatgct gtaaagggat
     3781 tttataccac tatccttata aagataaaga aactcacttt cagaaaatgc gtttttatgg
     3841 agaaatagga gcaaaggaac tggcaaaaaa gggcaaaaag agcaatcctg taaccgcact
     3901 tttaagagct gcagctaagt ttttaagaat gtttatcctt caacttggtt ttttagatgg
     3961 cagggcaggt tttttattat gcactcgttc agcctggggt gtatggtata agtatcacta
     4021 tctatggaaa ataagccaat aaaagttctg catattgatt ctgaaaagct ctggagaggg
     4081 ggacaacaac aggcagttta tctgtatgaa ggaatgcaaa aaaggggcat tccttgcagt
     4141 tttgtgtgcc aacctcaatc caaattagaa caatatttcc agaatcataa cttagtttat
     4201 aaatctattc ccttttcagg tgaaatggat ttaatggcag gatttcgttt atcaaagttt
     4261 gttcggaaac ataattacac catactaatt tgtcatagtg cccattcttt aagctggggt
     4321 ttgatagcta aagcttttta tcctgcttta aaaatcatcg gagtccgaag ggtggatttt
     4381 cctctaaaac agaatccttt ttctcgatgg aaatatacga attcaaaggt taataagata
     4441 gtggctgtat cccagaaaat aaaagaggtg cttagaaaag atggagtttc agaggataaa
     4501 attaaagtta tttatagcgg tattgattat aataaatatg cgcaggcaga agaagatacc
     4561 aattttagaa agcattttaa tattccccca gaagcaatat tagtaggcac tattgcagct
     4621 tttgcaggtc ataaggatta tcccactttt ttgaatgctg ccgctttagc acttaaaaac
     4681 agaccggact tatatttttt agcggttggt aagggagagc tgcaaaaatc tatgcaggaa
     4741 ctggcaaaag aacttaatat tgcggataaa gtgatattta ccggttttca gcaagaggta
     4801 gaaaagattc ttaaaaccct ggatatcttt gtcttagctt ctaaaaaaga aggattaggc
     4861 acctctattc tggaagctat ggcagcagga attcctgtta tagggacagc ttcaggaggc
     4921 attcctgagt taattaaaga cggagaaaat ggacttttag ttccatcttc caatccagaa
     4981 aagttaaaag atgctatctt aaaactggca aattctccag aattaagaat gcaactcgca
     5041 gaaagaggga aaactgaagc acaaaagttt gataaagaga ttatggtttc ccaatatatc
     5101 aagcttatac aagaattatg aaagagatag agcggatttt ctttattcag acagcctttt
     5161 ttggtgatgt gattatgagc actcccattc cgagagcatt aaaacaattg tatccaaatg
     5221 ccagactgga tatcattcta attccggaaa cccgcatcat ttatcaggat aatccgtatg
     5281 tggataatat ctatcttttt gataaacgaa atccattcaa gagaatccct tcttttttaa
     5341 gattagtgca caccttgcga aagaatcact atgatttagc agtctccgtg catatatcct
     5401 tttcttcttc attgcttatg cttttgagtg gagctaaaat ccgccttggt tatcccagac
     5461 agaaatttaa gaatttaacc attgatctgc cgaaaggtgt gcctgtagtc aaaagaggat
     5521 tgttattaat aaaagcttta agtgataggg aatttgatta tcaaacagaa ctctatatca
     5581 atcctgaaat tcaaaaaaag gtgcaggagt acattaatag taatagtctc aatcctgaaa
     5641 aaatgatagc tattgcacca ggttcggttt gggcaacaaa acgctggtta ccggaatatt
     5701 atgcagaggt agtgaaagcc cttacggaag aaggttatca atgtgtgatg ataggaagtg
     5761 ctgaagaaag gaaactttgt gaggaaatca taaaaatttc tggtgtgaat gcttataata
     5821 gtgcagggaa ttttaatatc ttgggttctg ctgaacttat aagaaattgt cgtctgatta
     5881 ttactaatga tagtgctcct ttgcatctgg caaatgccgt taaaacccca gttttggcta
     5941 tttttggtcc tactgtagaa agatttggtt gctttcctta tagagagaat gatagagtat
     6001 tgcaggtaga tttagaatgc cgtccttgtg gtaaacacgg acataaaaaa tgtccagaag
     6061 gtcatttccg ctgtatgaag gacattactg tagatatggt tctaaaaaat gctaaagaga
     6121 tgttaaatat aggggataaa gaggtgcaat gaaagagcta tatttaatcg ttggctacaa
     6181 tgatttcttt ggacaaacca gaaaaccctg ggttagtatt gatacttcaa gattagtatc
     6241 tgaactggag aatttagggt atatagttca taaaaatgag tttcacgaaa tagtgaatgg
     6301 caaagttaag attgagaatc aaatcgtgtt ctacactttc agtcatcgct taaatttgca
     6361 gcaatatatc agggattgtc ttttatatct attagattgt ggcaacactc tcattccttc
     6421 ttatgaacta ttttgctgtc acgaaaataa gggatggcag gttcttttaa gacgaaaact
     6481 gggcatagaa ggtttatgga atgcttattt tagttcaaaa agagaattaa aagattacga
     6541 gataaagtat ccagtagttt taaaaactat caccggttct aacgctaaag gagtttttct
     6601 ggttaaggat gaagaggatt tgttaaagaa gataaaaagt ctggagaagc aaccgtctat
     6661 agcgcagaaa ctggattttg tccgacgtaa gtattttcgt aaatataaaa agatacctgg
     6721 ttatccagat tatgatttat tcaaagatgc tcaactttat caggattata tcaccccaga
     6781 gattcctttt gtacttcagg aatttattcc caatcttacc tatgattatc gggttattgc
     6841 cttaggagaa aaatactttg tcagtaaaag gttaacgcgg aagaacgatt tccgtgccag
     6901 tggtgcgaaa aggtttactt ttgattttcc cgttcctgtg aaacttcttg atttcgccag
     6961 agaattgcat cataagattc actctcctta tctttctata gatgtgggag aaaatgaaga
     7021 gggagacctc tatctatttg agtttcaggt ggagcatttt ggtatcaatg ccatagtttt
     7081 gggtaaagga tattatgtgg aaaaagaggg gaagtgggaa tttgtagagg agaaaacccc
     7141 ctttgaaaaa gacctggctt atggtttaga cctttttcta agtag
//
LOCUS       DAORZF010000058         7136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_7423_length_7136_cov_393.884480, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000058 DAORZF010000000
VERSION     DAORZF010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 7136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7136
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7423_length_7136_cov_393.884480"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..239
                     /locus_tag="PK015_06475"
     tRNA            154..239
                     /locus_tag="PK015_06475"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:188..190,aa:Ser,seq:tga)
     gene            262..353
                     /locus_tag="PK015_06480"
     tRNA            262..353
                     /locus_tag="PK015_06480"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:297..299,aa:Ser,seq:gct)
     gene            362..436
                     /locus_tag="PK015_06485"
     tRNA            362..436
                     /locus_tag="PK015_06485"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:394..396,aa:Gln,seq:ttg)
     gene            447..520
                     /locus_tag="PK015_06490"
     tRNA            447..520
                     /locus_tag="PK015_06490"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:479..481,aa:Gln,seq:ctg)
     gene            complement(549..1559)
                     /gene="mltG"
                     /locus_tag="PK015_06495"
     CDS             complement(549..1559)
                     /gene="mltG"
                     /locus_tag="PK015_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOL34145.1"
                     /translation="MKPIKNLSHYLWMLILAIVLVLVIIVLWNIYAPVKSEEMLIKIK
                     KGDTAATIGEKLEQNGVIRSKFLFRTLAAMRGTDKHLIAGTYTIGGKHNLLQTLKMLE
                     EGNVTAVRVTIPEGFSMYQTLHRLERSGLARYEDLYKEATNPDFVYQLTGFKVKSLEG
                     FLYPDTYAFDPDVTVQEILSIMTRNFFNTLKKAGIDPYTIDNFYDKLILASIVEKESN
                     YPEERQLVAGVIYNRLRAGMKLASCSTVNYILEQRGIKKKVLSFEDTKIPSPYNTYIN
                     YGLPPTPICNPSLSSIMAALNPAQTDYYYFVSDRDGRNDFSRTAEEHLAKAKKYRRKE
                     WE"
     gene            complement(1556..1948)
                     /gene="ruvX"
                     /locus_tag="PK015_06500"
     CDS             complement(1556..1948)
                     /gene="ruvX"
                     /locus_tag="PK015_06500"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOL34146.1"
                     /translation="MDYGEKRIGLALSDPSRILAIPFKVIENKGFEYLVSEIESIINE
                     YEVTLVLVGMPYAIEGGDTPKTVETREVMSKLSNRLNITVQSWDERYSTDEAIKELIK
                     MGYDWKERRQLQDAMAAAMILKSYLENQ"
     gene            complement(1972..2559)
                     /locus_tag="PK015_06505"
     CDS             complement(1972..2559)
                     /locus_tag="PK015_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34147.1"
                     /translation="MTSPNRLSSLLLLSVFLLLTSCWNPFRPHLEDHSDTTIQNRTPQ
                     EVLENLELSYKERNINIYKDLLSEDFRFELIASEVNQIGVDVNSDGLRDDWWGYDQEI
                     LYTKNLFTTGSSDGSTPPPDELQLRLQIPPESNWQTDPQVGHENWVVIPCMFDLKLSY
                     YASNSMITASGVARFYLKPMNNRWYIAIWRDESNL"
     gene            complement(2543..2992)
                     /locus_tag="PK015_06510"
     CDS             complement(2543..2992)
                     /locus_tag="PK015_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34148.1"
                     /translation="MTNWDLCLQNLKYCYEDSRNAVKYISLFTEDFSFRFAAQDVNDY
                     NFPVSWNAQQEQDMLLNLFAWTDSVQVNLQEIVSQPDNITATEAKIYRQYELIRYPSD
                     KSVPQEYQGELELHFIKSGAYWYIRKWFDYRLGSYPTWGKLKYDFSQ"
     gene            complement(3292..3801)
                     /locus_tag="PK015_06515"
     CDS             complement(3292..3801)
                     /locus_tag="PK015_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34149.1"
                     /translation="MPDKANNRKKEANKPAPAKKTNNMEMKEGNSFGIVELLIFIILA
                     GVIFIFIFGMKQMQEEKEKELLAEKKFEQVLPNFALISNAAKDYQKNDPFASWPSMLE
                     EIIDPSKVNTPEFKFSFQENGTVIMTTTKEFGKEGIKVVYDINKDTFEIDDPNPNVKP
                     TIKEDWIYQ"
     gene            4142..6937
                     /locus_tag="PK015_06520"
     CDS             4142..6937
                     /locus_tag="PK015_06520"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOL34150.1"
                     /translation="MKKSMTILLALTMLIFNLSATMFCPDTGETIPGFANSNYFEQST
                     PENIDTGAKDVNSWSGTGPWGGNIRYLTTDPTNPNRVFAASGSSLTAVEGGVYYSIDG
                     GINWLPTTLPRKAYNAIASSPSQPGVFYAGARNGLYKSTDYGITWQAAGQSSSYILGL
                     GVQANNGNVIVMGKSGNVGFLVSNDGGATFNSVGVNSGFMRHFAWHENNPQRMYVVMG
                     SSTSSVLTSVDNGQTWTNFGPAGDGWGMYASPTDSLFCLIAHANGIYRTTDGGLNWTN
                     VLGGTFRNVTEFNGSYFASSNAGGIFESNDQGLTWQVTPHPVVQSTWQAATSTSAGAL
                     FGHWGGIFRVTGYQQPIVPSHTGINLALVHGLAYYADTNELWGGTEGSGLYCSTDNGL
                     TWEHKVNGLNNWMVYELQPTNHQFYHSGRMLVGTLDGAYTSMDGGNTWSYVYFQGTQV
                     SACEVHPTNPNIFWLGSASGEIKYTTDGGQTFNTSGGGYFGYAPRLKLGKAPDGNLRL
                     FLCFQGSATAVWYSDDGGANFVPTTGLESTTYQPMVSIRPILGDQPQIIYASTNSGVF
                     KSTDNGATYSLTGMTGFSWSVLAGPGQQVVSGKDNGVSYSTNEGTTATSITQNLESNA
                     TIWQIAWGSSTNQLYIATRGRGILENRFDSEEYLPPTNLTANPLDSAILLNWTPPAGT
                     LLPVSYIIWRDAYPLVSLLASQTSYTDSGLINGQEYKYFVSAVYENDIHTSSIQIITA
                     IPAIPTYQPVVNLTAEVSNYDVILSWTAPGAGETGYKIYRDDNLIYTITDPSITSYTD
                     TGLTAGNYLYGVTATYPTGESAPVTVSVTVVSNYDPNIPGLITELKSCYPNPFQNSTS
                     IHYSVKEQVPVEIRIYNARGELIRKLVNDNRAPGNYNIVWDGRMQNGSRAPNGIYFYQ
                     MKAGNFNSQPKKLLLMK"
BASE COUNT         2120 a         1395 c         1316 g         2305 t
ORIGIN      
        1 acctgtttca cttttttgca gtaagagaga aacaaattaa acagttcttt aaaagtcaaa
       61 ctgataaatt taagtgaaaa tattttaata aacaacggaa agaaactgat tgacaaaaaa
      121 ggctgtttaa gatgaagaga taaaaatggt gagggaggat tagtcctaat tggtaaggca
      181 gcggtcttga aaaccgccgg gttttaccca tgggggttcg agtccctcat cctccgccag
      241 taaatatagt gttttttaaa tggagaggtg accgagcagg tttaaggtgc acgcctgcta
      301 agcgtgtgtg ggtcaaaagc tcaccgtggg ttcaaatccc accctctccg ccagaatttg
      361 ttgggcagtc gccaagcggt aaggcagcgg gttttggtcc cgccattcgg gggttcgaat
      421 cctccctgcc cagccagatt tttttatggg atgtcgtcta atggtaggac agcggactct
      481 ggatccgtat gtgagggttc aactccttcc atcccagcca ttttccctcc agtcatagga
      541 ctggtttttt attcccactc ttttctacga tatttcttag ctttagccaa atgctcttct
      601 gcggtacgag aaaaatcatt tcgcccatcc ctatcagaaa caaaataata ataatctgtc
      661 tgggcaggat tcaatgcagc cataatagat gaaagtgatg gattgcagat aggagttgga
      721 ggcagtccat aatttatata agtattataa ggagaaggaa ttttggtatc ttcaaaagac
      781 aggactttct ttttaatgcc tctttgttcc aggatgtaat ttacagtaga acaggatgcc
      841 agtttcattc ctgctcttag acggttatat ataactcccg ctaccagttg cctttcttcg
      901 ggataattac tttccttctc tacaatgctt gccagaatta atttatcata aaaattgtct
      961 atggtataag ggtctatccc tgctttcttt aaagtattaa aaaaattcct cgtcataatt
     1021 gataagattt cttgaacggt aacatctggg tcaaaagcat aggtatcagg atagagaaac
     1081 ccctccaggg atttgacttt gaaaccagtg agttgataga caaaatccgg attagtggct
     1141 tccttataga ggtcttcata tctggctaat ccacttctct ccaatctatg cagagtttga
     1201 tacatagaga acccttcggg aatagtcact ctaactgcgg taacatttcc ttcttccagc
     1261 atttttaagg tttgtaaaag gttgtgtttt ccaccaatgg tataggttcc agcaattaaa
     1321 tgcttatctg tacctctcat cgcagcaaga gtgcgaaaca gaaatttact tcggattact
     1381 ccattttgct ctaatttttc cccaatggtg gcagcagtgt ctcctttttt tattttaatc
     1441 agcatttctt ctgattttac tggagcatat atattccaga gcacaattat tactaagaca
     1501 agaacaatgg ctaaaattag catccaaaga tagtgagata aatttttaat tggtttcatt
     1561 gattttccag ataacttttt aatatcatag ctgctgccat agcatcctga agctgtcggc
     1621 gttctttcca atcatagccc attttaatca attccttaat tgcttcatcc gtgctatatc
     1681 tttcatccca ggattgaaca gtgatattaa gacgattaga caatttagac ataacctctc
     1741 tggtttcaac tgtcttagga gtgtctccac cttcaatagc atagggcatt cctaccaaca
     1801 ccagggtcac ctcatattca tttataatag attcaatctc ggatactaaa tactcaaatc
     1861 ctttattttc tataacttta aaaggaattg caagaatgcg cgaaggatca gataaagcaa
     1921 gacctatcct tttttctcca taatccaggg caagaattct tccctctcgt tttacagatt
     1981 ggattcatcc ctccaaattg ctatatacca tctattattc attggtttaa ggtaaaaacg
     2041 agctactcca ctggcggtaa tcatagaatt ggaagcataa taactaagtt ttaaatcaaa
     2101 catacagggt ataaccaccc aattttcgtg tcccacctga gggtcagttt gccagttact
     2161 ttcaggtggt atctgaagcc gtaattgtaa ttcatctgga ggaggtgtgg aaccatctga
     2221 agagcctgta gtaaagagat ttttagtata taatatctct tgatcatagc cccaccagtc
     2281 atctcttaat ccatcactat taacatccac ccctatctga ttaacttctg atgctatcaa
     2341 ttcaaagcgg aaatcttcgg aaagcaagtc cttgtagatg tttatatttc tctccttata
     2401 ggataattcc agattctcca gcacctcctg aggagtacga ttttgaatag tggtgtcaga
     2461 atggtcttcc agatgcggtc tgaaaggatt ccagcaactg gtcagtaata aaaatactga
     2521 tagcaataaa agagatgaaa gtctattggg agaagtcata tttcaatttc ccccaggttg
     2581 ggtaacttcc caatctataa tcaaaccatt tacgaatgta ccaataggca ccagatttaa
     2641 taaagtgaag ttccagttca ccttgatatt cttgcgggac agatttatcg gaaggatagc
     2701 gtataagttc atactggcga tagattttag cttcagtggc agttatgtta tctggttgag
     2761 agacaatctc ctgcaaattt acctgaacac tatccgtcca ggcaaaaaga ttcagaagca
     2821 tatcttgctc ctgctgagca ttccagctga ccggaaaatt ataatcatta acatcctgag
     2881 cagcaaaacg gaaactaaaa tcttctgtaa acagacttat atatttaacc gcatttctac
     2941 tatcttcata acaatactta agattctgca aacatagatc ccagttcgtc atataactat
     3001 tccatggtgg tggatttatt ggtttctcgg cttcccgtaa atcaaaaaga ttacaggaac
     3061 tgagagttat aataaataat aggagcggta taagtttttt catattttca atataagtct
     3121 atatacttta ttgtcaatag atattttacc cttcaagtta ttgatttagt tagctaaaaa
     3181 tacagctatc ttcctaaatg agtgagagtt ctataaatta tcccaaagag acaaaaacaa
     3241 ccctctttaa tggagggttg ttttaatatt attacattta tcaggttagt gttattgata
     3301 aatccaatct tctttaatgg tgggtttcac attcggattg ggatcatcta tctcaaaggt
     3361 gtctttgttg atatcgtata ccaccttaat tccttctttg ccaaattctt tagtagttgt
     3421 cataatcaca gttccatttt cttgaaacga gaacttaaat tcaggagtat taactttaga
     3481 ggggtcaata atttcttcca gcattgaggg ccaggaagca aatggatcat tcttttgata
     3541 atcttttgcg gcattactaa taagggcaaa attaggcaac acctgttcaa atttcttttc
     3601 tgctaaaagt tctttttctt tctcttcctg catctgcttc atcccaaaga taaagatgaa
     3661 gataactccc gctaaaatta tgaaaattag caattcaacg ataccgaagg aattaccttc
     3721 tttcatttcc atattatttg ttttcttagc tggagcaggt ttatttgctt ctttttttct
     3781 attattggct ttgtcaggca ttatacctcc aaaacataaa tccttttttt cacttttata
     3841 atattatgat ttttgtcaaa gtatttgttt gatttcatta gcaaattgct aatttaatag
     3901 cccgttccca acaatgttct ggattttctc ttcaaaaata tgttttcccc taagattggc
     3961 ttctttcttg catatataat ttagaaaatc aacttttcca aagtagaaaa attaaattgc
     4021 cttattttaa tactcttttg aaaaaagctt taatgtctaa gataggttca accctactaa
     4081 gttctaatct aaaaaagatg aaacaatttt attttaaatc ataattaaaa ggagataaaa
     4141 gatgaaaaaa tcaatgacca ttctgctggc gttgacaatg ctgatattta atttatcagc
     4201 aactatgttt tgtcctgata ctggcgaaac cattcctggc tttgccaatt ctaattattt
     4261 tgaacaatct actccagaaa atattgacac aggagcaaaa gatgtcaatt cctggagtgg
     4321 aacaggaccc tggggaggaa atattcgtta cctcaccact gatcccacta atcccaatcg
     4381 tgtttttgct gccagtggtt caagtttaac tgcagttgaa ggtggagttt attacagtat
     4441 tgatggcggt ataaactggc ttcctaccac tttaccccga aaagcttata atgcaatagc
     4501 ttcttctccc tcacaacctg gagtttttta tgctggagct cgcaatggac tttataaaag
     4561 cactgattat ggcataacct ggcaagccgc tggacaatct tcatcttata ttttaggttt
     4621 aggtgtccaa gctaataatg gtaatgtaat tgtaatggga aaatccggta atgtcggttt
     4681 tttagtttct aatgatggag gtgcaacctt caattctgtt ggtgtaaact ctggttttat
     4741 gcgtcatttt gcctggcacg aaaacaatcc tcaaagaatg tatgtagtaa tgggaagttc
     4801 cacttcttct gtgttgacca gtgtagataa cggtcaaacc tggactaatt ttggtccagc
     4861 aggagatgga tggggtatgt atgcttctcc tactgatagc cttttctgct taatagctca
     4921 cgcaaatgga atatacagaa ctactgatgg aggattaaac tggactaatg tgctgggtgg
     4981 cacttttcgc aatgttacag aattcaatgg ttcatatttt gcctcctcta atgctggggg
     5041 aatttttgaa agcaatgacc agggtttaac ctggcaagtt acacctcatc cagttgttca
     5101 atctacctgg caagcagcca cttctaccag tgcgggagca ctttttggac actggggtgg
     5161 aatatttcgc gtcacaggtt atcaacaacc tatagttccc tctcatacag gaatcaatct
     5221 cgctctggta cacggtctgg cttattatgc tgatactaat gaactctggg gtggaactga
     5281 aggaagtggt ctctattgca gcactgataa tggtctaacc tgggaacata aagtaaacgg
     5341 attgaacaat tggatggttt atgaattgca accgaccaat caccaattct atcattctgg
     5401 cagaatgttg gttggaactt tggatggtgc ttacacttct atggatggag gaaacacctg
     5461 gtcttatgtg tatttccagg gtactcaggt ttctgcctgc gaagtccatc ctactaaccc
     5521 aaatattttt tggcttggct ctgcttctgg tgaaattaaa tatacaacag atggtggtca
     5581 aacctttaac acttcaggtg gcggatattt tggatatgca cctcgtttaa aattgggaaa
     5641 agctcctgat gggaacttac gtctcttctt atgtttccag ggaagtgcta ctgcagtttg
     5701 gtattctgac gatggaggtg ctaatttcgt tcctactacc ggattagaaa gcaccactta
     5761 tcaacctatg gtctctatca gaccaatttt gggagatcag ccacaaatta tttatgcttc
     5821 cacaaactca ggagttttta aatcaactga taatggagct acctactctt taactggaat
     5881 gactggtttc agttggagtg ttttagccgg accgggtcaa caggttgttt caggaaagga
     5941 taatggagtt agttatagca ctaatgaggg gactactgct acctctataa cccagaatct
     6001 ggaatctaat gccacgatct ggcaaatagc ttggggtagc agtaccaatc agttatatat
     6061 cgccactcgt ggaagaggca tcctggaaaa tcgctttgat tcagaagaat atctacctcc
     6121 tactaatcta actgcaaatc ctctggatag tgcaatcctg ttaaattgga ctcctccagc
     6181 aggaacatta cttcctgttt cctatattat ctggcgagat gcctatcctc ttgtatctct
     6241 tcttgcttct caaacctctt ataccgattc aggtttaatc aatggacaag aatataagta
     6301 ttttgtatct gcggtttatg aaaacgatat acatacttca agtatacaga taatcacagc
     6361 aatacctgca attccaactt atcaacctgt ggttaatcta acggctgaag tttccaatta
     6421 tgatgtgatt cttagctgga ctgcacccgg agctggtgaa actgggtata aaatctatag
     6481 agatgacaac cttatctata cgataaccga cccttctatt accagttaca ctgatactgg
     6541 acttactgca ggcaattatt tatacggtgt tacagcaact tatcccactg gagaatctgc
     6601 accagttaca gttagtgtta ctgttgtatc caattatgac ccaaatatac cgggattaat
     6661 cacagaactg aaatcctgtt atcctaatcc tttccagaac tctacctcta ttcattactc
     6721 agtaaaagaa caggtaccag tggaaatacg tatttataat gcccgaggtg aattaatccg
     6781 taaattggta aatgataaca gagcaccagg aaattataat attgtttggg atggacgcat
     6841 gcagaatggt tctcgtgctc ccaacggaat ctatttctat cagatgaaag caggtaactt
     6901 taatagccaa cccaaaaaac tgctactgat gaaataagca tataagataa gctctatttc
     6961 ataaaaaagg aaaagattga acataccttg atcttacctt aattatagaa atgaaaagga
     7021 taggaattat ctttcagaag agcataactc tctctgatta aagatttctt catccttttc
     7081 atttctttta ccatttatat attaattttt agggttcatt atcatcaatc ggtgtg
//
LOCUS       DAORZF010000059         7018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_7563_length_7018_cov_345.857389, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000059 DAORZF010000000
VERSION     DAORZF010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 7018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7018
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7563_length_7018_cov_345.857389"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..727)
                     /locus_tag="PK015_06525"
     CDS             complement(41..727)
                     /locus_tag="PK015_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34151.1"
                     /translation="MKPLYIIIGAYGSGKSEFSIHLAKSLNQPDNKVVLADMDVVNPY
                     FRTREVQEEFAQIGIKVIAPEGAFKHADLPMLSPSIKGAIENYEETVILDVGGDPVGC
                     KILGRFQTEIETRGYQMLFVVNTYRPFTTNVDEILSMKAQMEASSRLKVTEFICNNNL
                     MEYTTQEVVEEGIKILNECSEKTGLPFNYYLVLDLYQDRIPDGLMGKKRIIMNYTFRK
                     PWEYYVMKGI"
     gene            complement(724..1383)
                     /locus_tag="PK015_06530"
     CDS             complement(724..1383)
                     /locus_tag="PK015_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOL34152.1"
                     /translation="MKEYQAIIFDMDGTLIDSMYLWREVDKEFLQKRGFSVPVDLFHH
                     LPQGNSFIQTAQYFKDRFGLADSPESIMQEWNDMVSWHYENEVKLKPGAKEVLEKLYQ
                     KKVPLGLGTSNSYDLAKKVLTRNGVWDYFSAVVTGDMELLGKPYPDIYLECARQLKIP
                     PEYCLVIEDTLPGIQAAKAAGMKSIAIYDEDSLEFHPQMQVLADRFVYNFKELENILN
                     L"
     gene            complement(1380..3266)
                     /locus_tag="PK015_06535"
     CDS             complement(1380..3266)
                     /locus_tag="PK015_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34153.1"
                     /translation="MLSAQNTIAHLEGEALVLPESWSFRYDPAQVLVKIKVEADSTLS
                     LIKVMDGKEELTSLIQEFLSRCKANLLSIPVSTDTEVNVVIDLIQVPPSYITYMTHGK
                     TGNLKDIEKWITEERKSLSFHQPSQDSVAANGFTSLKEPYRSGYFFYSRPRYDDYPNL
                     YGFKLQNSLYSSSFFQNLTLGFFDFEEEIMELNGTEKYYPYQATLSDIEVGIGDYEHL
                     FTKVALKKNNLFEYKDLYLSLDFLTQTGYWLEEDASRNSLKLHLSVPIGKTNLKFSWL
                     DQNSKLSMLNLRPEFWLPQNFIVKDHYNALYAGFNWIGPNLAILYEQNDIKSDKFIKN
                     LHNEALHLQAWKDLQIQKVELALKYEHIISDYNYETGEPDYKDRAEMQFRWNHNPLKW
                     DWKTELTDFQQVQVATDFSYSFNKFRFGAFACGNFNPVESSLWIPSIYSETDSLRNVQ
                     IREINNAGVYTSYSFKAPSYLTLSLGRRIVEDIYPEMETELKEKKELFYIKFVAELDK
                     IWDKWEIKWQPALIWQNNYAGLFEEPEFEGCSYLNLFYHLPYNNALFAGFSLSGHSGY
                     WNSDPSTFFVDSSSIVDLWAGVQISNRFEFQVTYKNLMDTSIYGVYPVPPSIYASVRW
                     FFLN"
     gene            complement(3317..4288)
                     /locus_tag="PK015_06540"
     CDS             complement(3317..4288)
                     /locus_tag="PK015_06540"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOL34154.1"
                     /translation="MRIGITGANGFAGSNFANYFQSQGYEVRGIVRPHADLSLLDPNL
                     DIQFTDYNSQDELKRVLEDVDVLIHNAGAVRTHTFMQMIEANVGTTRRVMEAFNHSSK
                     GKRFIYISSQAASHPSRDMELAKEEDPSAPVDWYGKSKVLAEKIIKTECVKEWVIVRP
                     CSIYGPGERDFYQVFKAVQKGINFHIGRKPQYMNLIYVRELAQFLELCLTQKTAVGEI
                     FFASDGEIYTQQDFMHLIAEILKVKTFEIAVPVPLAKTAFYAGEAFERITGKTTLLNK
                     QKMKEIVDVTWLCSIEKAKRLLGWNPQPNLREHLSETIQWYKEHNWL"
     gene            complement(4318..4393)
                     /locus_tag="PK015_06545"
     tRNA            complement(4318..4393)
                     /locus_tag="PK015_06545"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4358..4360),aa:Val,seq:tac)
     gene            complement(4409..4486)
                     /locus_tag="PK015_06550"
     tRNA            complement(4409..4486)
                     /locus_tag="PK015_06550"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4449..4451),aa:Pro,seq:tgg)
     gene            4949..>7018
                     /locus_tag="PK015_06555"
     CDS             4949..>7018
                     /locus_tag="PK015_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34155.1"
                     /translation="MKYSCFIIFYLLLTLSIQALVVPVSDTPVLQDDNMLTNFVRNDP
                     DDKKPEENPTKVWLWQQGNNLMIHFSCPIDSQFVVGPVEVRDEAMFSDYVSVQLITLP
                     YAYFAYYYTGYPNGNLVDGIRKEDMSVDLNWDSKYSYKTSYDDKNWELTMQIPLGELR
                     FKSDLPYNWYIILSRYKYATTEYYCSPYFNTNQKKDYFTAGHKIQLNQKITREFNLDF
                     KPYFVKSYDLIEKTSSYDPDMVGLDIAYHPNPTTKIKLSMNPDFSEVPPDDAQDIYNL
                     KYPPYYDENRFFFIEDLDAFGIFSDFFYSRNIVQPQLAYKATGSLGKTRWGILGAWDK
                     KIVSGGELINPDDYFQLLSVIPTIGNFTMGNAFIARTNTGYYNYVYSGVYEYRLSKVL
                     TLNTYLAGSLRKNENEEDNSLKKGYLGQIGINYYPGNWENSLNIAKISKDIFADAGYL
                     YGKDRLGYSLSSYWQSNPFPGFLQLVSVLGMAELQDLHPDDDKKTEYDLSGMLTLRFK
                     PKFFYTFRPGYYSIYDNINQKHNVFSMHNILNFKCWKNFSLYINWITGETLVYALNKT
                     SQMNTITFRIIGTPQKSLYYQVQGNLVSYDYPKENIIDINGVPLTLYLDNQYAIINAS
                     LRFTPVQRFRFTLGGSITTYETPTSYSNFNYYSNLRYEFKPNYFIYLGLKNNLLQDEP
                     VTLDEPLG"
BASE COUNT         2232 a         1335 c         1140 g         2311 t
ORIGIN      
        1 actaaactaa ggatttactt ttaaaaaggg gtacccttat ttatattcct ttcatcacat
       61 aatattccca gggttttcgg aaggtataat tcataatgat gcgttttttg cccattaaac
      121 catcaggaat gcggtcttga taaaggtcca gcacgagata atagttaaat ggcaaacctg
      181 ttttttcact acattcattg agtattttta tcccttcttc cactacttcc tgggtagtat
      241 attccataag gttattattg catataaatt cagtaacttt cagtctggaa gaagcctcca
      301 tttgtgcttt catagataag atttcatcta cattagtagt gaaaggacga taggtattaa
      361 ctacaaaaag catttgataa cctcgagttt ctatttcagt ttgaaatctt cccagaattt
      421 tacatccaac agggtcacct ccaacatcca gaataacggt ttcctcatag ttttcaatag
      481 cgcccttgat acttggtgaa agcattggta aatcggcatg tttgaaggca ccttcagggg
      541 caataacttt aattcctatt tgagcaaatt cttcttgtac ttcacgcgtg cgaaaatagg
      601 gattgactac atccatatca gctaaaacca ctttgttatc aggttgattg agactttttg
      661 ccagatgaat actgaattca cttttaccgc ttccataagc tccaatgatt atgtataaag
      721 gtttcataag tttaaaatat tctccaattc tttaaaatta tagacaaatc tatctgccaa
      781 aacttgcatt tgaggatgaa attccagact atcttcatcg tagattgcta tagatttcat
      841 acctgctgct ttagcagcct gaattccagg tagagtatcc tcaataacca aacaatattc
      901 aggtgggatt tttagttgtc tggcacattc cagatagata tcaggatagg gtttacccag
      961 aagttccata tcacctgtaa ctacagcaga gaaataatcc cagactccat tccgagttaa
     1021 aacctttttt gctaaatcat aggaattgct tgttcccaga cctaatggta cttttttctg
     1081 gtataatttc tccagcactt cttttgcgcc aggtttaagt ttcacttcat tttcataatg
     1141 ccagcttacc atatcattcc attcttgcat aatactttca gggctatccg ctaatccaaa
     1201 tcggtcttta aaatattgtg ccgtttgaat gaaagaattg ccctggggaa gatgatgaaa
     1261 taggtcaaca ggcactgaaa atcctctttt ttgtagaaat tctttatcta cttctcgcca
     1321 tagatacata gaatctatga gggtcccatc catatcaaag atgattgctt gatattcttt
     1381 caattgagaa aaaaccaccg aacagaagca taaattgaag gaggaacagg ataaacacca
     1441 tagatggagg tatccattag gttcttatag gtaacttgaa actcaaatct attgctaatt
     1501 tgaactccag cccataaatc tacgatagaa gaagaatcca caaaaaaggt ggaaggatcg
     1561 gaattccagt aaccactgtg cccagataga gaaaatccgg caaagagtgc attattataa
     1621 ggtaaatgat agaaaaggtt aagatagctg catccttcaa attccggttc ttcaaataat
     1681 cctgcataat tattttgcca gattagagcg ggttgccatt tgatttccca tttatcccaa
     1741 attttatcca gttctgcaac gaatttgata taaaaaagct cctttttctc ttttaattcc
     1801 gtttccattt cgggatagat atcctctaca atccttcttc caagagaaag agtcagataa
     1861 gaaggtgctt taaaagaata gctcgtataa actccagcat tattgatttc tcttatctgg
     1921 acattcctga gagaatctgt ttcgctataa atacttggaa tccacaaaga agattccacc
     1981 ggattaaaat tcccgcaagc aaaagcacca aatctaaact tattaaaaga gtaagaaaaa
     2041 tccgtagcca cttgaacctg ttgaaaatcg gtaagttcag ttttccaatc ccattttaga
     2101 ggattatgat tccagcggaa ttgcatttct gctctatctt tataatctgg ttctccagtt
     2161 tcatagttat aatcagagat gatatgctca tatttcaacg ccagttcaac tttttgaatc
     2221 tgtaaatctt tccaggcttg aaggtgtaaa gcttcattat gcaagttttt aataaattta
     2281 tcggatttta tatcattttg ttcatataaa attgccaggt tgggtccaat ccaattaaat
     2341 cccgcatata aagcattata gtggtctttt acgataaagt tttgaggcaa ccagaattct
     2401 ggacgaaggt tgagcataga aagctttgaa ttttggtcaa gccagctaaa tttaagatta
     2461 gttttcccga taggcacact aagatgaagt tttaaggaat tgcgactggc atcttcttca
     2521 agccagtatc ctgtttgagt gagaaaatct aaacttaaat ataaatcctt atattcaaag
     2581 agattattct tctttaaagc tactttggta aataaatgtt cataatctcc aattcccact
     2641 tctatatcac ttaaagtagc ttgataagga tagtattttt ctgtgccatt gagttccata
     2701 atctcttcct caaaatcaaa gaaaccgaga gtcaagttct ggaaaaaaga ggaagagtag
     2761 agcgaattct gtaatttaaa tccataaaga ttaggataat catcgtatcg tggacgggaa
     2821 tagaaaaagt atccagagcg gtagggttct ttaagcgatg taaaaccatt ggcagcaacg
     2881 gaatcttgac tcggttgatg aaagcttaaa gattttctct cttctgttat ccatttttct
     2941 atatctttta aattgcctgt tttgccatga gtcatataag tgatataaga aggaggaacc
     3001 tgaattaaat caatgactac attcacttca gtatcagttg aaacggggat agacaaaaga
     3061 tttgccttgc atcttgaaag aaattcttga attaaagaag taagttcttc ttttccatcc
     3121 ataactttaa ttaaacttaa agtgctatct gcttccactt taattttaac cagcacttga
     3181 gcagggtcgt aacgaaaaga ccaggattca ggaagtacta atgcttctcc ttccagatga
     3241 gcaatagtgt tttgagctga taacattggg acaagaaaca aaaatagaaa aaaacaaata
     3301 gattttaatg acactctcat agccagttat gctctttata ccactgaatg gtttcactta
     3361 aatgctctct tagattgggt tgcggattcc aacccaataa tcttttagct ttttctatac
     3421 tgcaaagcca agtaacatca actatttctt tcatcttttg tttatttaag agagtagttt
     3481 tacctgttat tctttcaaat gcttctcctg cataaaaagc cgttttagcc agaggaacag
     3541 gtaccgctat ttcaaaggtc ttgaccttta atatttctgc aataagatgc ataaaatcct
     3601 gttgtgtata aatttcacca tcagaagcaa aaaagatttc gcctactgca gttttctgag
     3661 tgagacagag ttccagaaac tgtgccagtt ctcgcacata gataaggttc atatattgag
     3721 gttttcttcc tatatggaaa tttatccctt tttgaacagc tttaaagacc tgataaaaat
     3781 ccctttcacc aggtccataa atagagcagg gtcgcacgat aacccattct ttaacgcatt
     3841 ctgttttaat gattttctct gcaagaacct tgcttttgcc ataccaatca actggtgcag
     3901 aagggtcttc ttccttcgct aattccatat ctctggaggg atgagaagcc gcttgggaac
     3961 ttatatagat gaatcttttg cctttagaac tatgattaaa tgcctccatc actcttctgg
     4021 ttgtccctac attggcttct atcatttgca taaaagtatg agttcttacg gcaccggcat
     4081 tatgaattag gacatctaca tcttccagaa ctctttttaa ctcatcttgg gaattgtaat
     4141 ccgtgaattg aatatccaga ttaggatcca gtaaactgag gtctgcatga ggacgaacta
     4201 tacctctaac ctcgtatcct tgggattgaa aataattggc aaaattgcta cccgcaaaac
     4261 cattagcacc tgttatacct attctcatta cacatccaga acttaattga taaaatatgg
     4321 tgggcgctaa gggactcgaa cccccgacat catgcgtgta aggcatgcgc tcttccagct
     4381 gagctaagcg cccttattct ttttattatg gtcggggtga gaggatttga acctccgact
     4441 cctggttccc aaaaccagtg cgctaaccgg gctgcgctac accccgaatt tttatccaat
     4501 taaaactgaa ttcattaatt gtcaagctga aaattcccta cttaaataat tatcatctat
     4561 tgctatataa ctttatttgt ctgctttctt aattcccctc aatattttat tctttaacca
     4621 taaatttctc ttttctttat cattctttca ctcctataag ttaaaattcc ccttggttta
     4681 taaccatttc ctccatcttc atcccatata aaatactaat gtccaataaa tcttgaactt
     4741 atgtcccatt tcggacataa gctggacata agctggacat aaattggaca taaggaaaga
     4801 ggataatttt aaaattgaaa aaggtttcta atatcagaat taataaaata aaaactattt
     4861 ttaaaacttt tacttgacag aacttgcttt aatccgatag ggttttttaa tgtcttaaaa
     4921 ataatgatag aaaggtagtt aatttgaaat gaaatattct tgttttatta tattttacct
     4981 tttactcact ttatctatcc aagctttagt agttcctgtc tccgatactc ccgttctaca
     5041 agatgataat atgctaacca atttcgtgcg aaatgaccca gatgacaaaa aaccagaaga
     5101 aaatcctact aaagtatggt tatggcaaca gggaaataat cttatgatac atttctcttg
     5161 tccgatagac tcacaatttg tagttggtcc tgtagaagtt agagatgaag ctatgttctc
     5221 tgattatgta agtgttcaac tgattacact tccctatgct tattttgcct attactacac
     5281 aggttatcct aatggcaatt tggtggatgg aattcgtaaa gaggatatgt ctgttgattt
     5341 aaattgggat agcaaatata gctataaaac ctcttatgat gataagaact gggaacttac
     5401 aatgcaaata cctctgggtg aattgagatt caaatctgat cttccctaca actggtatat
     5461 aattcttagc cgctataaat atgccactac tgaatattac tgttctccct atttcaacac
     5521 taatcagaag aaagattact ttactgctgg tcataaaatc cagctaaacc aaaagattac
     5581 ccgggagttt aatttggatt ttaaacccta ttttgttaaa agttatgatt taattgaaaa
     5641 gacctccagc tacgatcctg atatggttgg tttggatatc gcttatcatc ccaatcccac
     5701 taccaaaata aaacttagta tgaaccctga cttttcagag gtccctccag atgatgccca
     5761 ggatatctat aatctaaaat atccccctta ttatgatgaa aatcgtttct tctttataga
     5821 agatcttgat gctttcggaa ttttctccga ctttttttat tcccgaaata tagttcaacc
     5881 tcaattagcc tataaagcaa cgggaagttt aggaaaaacc agatggggaa ttctcggtgc
     5941 ttgggataaa aaaattgtaa gtggaggcga attaattaat cccgatgact attttcagct
     6001 tctctctgtt attccaacta tcgggaattt cactatggga aatgctttca tagccagaac
     6061 taataccgga tattataatt atgtctattc aggtgtctat gaatataggt tatctaaagt
     6121 gcttacgctg aatacttacc ttgctggaag cctccgcaaa aatgaaaatg aagaagataa
     6181 ttccctcaaa aaaggatacc tgggacaaat tggaattaat tattatcctg gcaactggga
     6241 aaattctctt aatattgcaa aaataagtaa agatatcttt gctgatgctg gatatttata
     6301 tggaaaagat agactgggat attccttatc ttcttactgg cagtctaatc ccttccctgg
     6361 ttttcttcaa ttagtatctg ttttaggaat ggcagaattg caagatttac atcccgatga
     6421 tgataaaaaa actgaatatg acctttctgg aatgttaact ctaagattta aacctaagtt
     6481 tttctacacc tttagacctg gttattatag tatttatgat aacatcaatc aaaaacacaa
     6541 tgtatttagt atgcataata ttttgaattt taaatgctgg aaaaattttt ctctatatat
     6601 aaattggata acaggagaaa ctctggttta tgccttaaat aaaacctcac aaatgaatac
     6661 tatcaccttt aggataattg gcactcctca aaaaagtttg tattatcaag ttcaaggaaa
     6721 tcttgtatca tatgactatc ctaaagaaaa tatcattgat ataaatggtg tccccctaac
     6781 tttatacttg gataaccaat acgctataat caatgcttct ctaagattta cccctgtgca
     6841 aagattcaga tttaccctcg gaggaagcat aactacttac gaaactccca cctcctattc
     6901 caattttaac tattattcca acctccgcta tgaattcaaa cccaattact ttatctacct
     6961 tggcctaaaa aataatctgc ttcaagatga acctgtgact ttggatgaac ctctcggc
//
LOCUS       DAORZF010000060         6863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_7770_length_6863_cov_376.261016, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000060 DAORZF010000000
VERSION     DAORZF010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 6863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6863
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7770_length_6863_cov_376.261016"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..92
                     /locus_tag="PK015_06560"
     tRNA            18..92
                     /locus_tag="PK015_06560"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:50..52,aa:Lys,seq:ttt)
     gene            97..171
                     /locus_tag="PK015_06565"
     tRNA            97..171
                     /locus_tag="PK015_06565"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:130..132,aa:Glu,seq:ttc)
     gene            195..270
                     /locus_tag="PK015_06570"
     tRNA            195..270
                     /locus_tag="PK015_06570"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:229..231,aa:Glu,seq:ctc)
     gene            342..1274
                     /locus_tag="PK015_06575"
     CDS             342..1274
                     /locus_tag="PK015_06575"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOL34156.1"
                     /translation="MKDENKMPDLEPEIFPDRRIKISFESLGNLHSFKVQPWLLYVIL
                     LVIVIGIIFLFVASFRGPDKENLNKIHRLETENQYLQSELEKFNVQLDSINALLDSLR
                     VKSAHKKINYPSYSNNLQPINKLLVHPALDSKLSAIEIKLEVIKNRLTYLDSNGVSSK
                     DDELSSDALKNIPSILPTFGEISSYWGLRNHPILGGEIWHQGIDIANRTGTPVYATAE
                     GIVSRVESDDGWGNVITIDHKGGYRTLYGHLYRSKVHLGDIVRKGQIIGLMGSTGMST
                     GSHLHYGVYLNGAPINPSYYLNRQDLSYYNMVRK"
     gene            1271..1915
                     /locus_tag="PK015_06580"
     CDS             1271..1915
                     /locus_tag="PK015_06580"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOL34157.1"
                     /translation="MKKEADIAWLFTTTSPKEIFSSYKNIDFSKDLIIAVDAGLERVN
                     KLGIEPDLIIGDMDTLSPELLEQYPDTEKIKYDIQKNHTDTELAILWCLEHNLKQVVI
                     INGLTNRFDHSLGLLQNLALLHSKNVTARIESERQVVFFLTNKSHLNGYEGCLLSLFP
                     YGGDATIKNSEGLFFPLKNLTLSPLHTRGISNNVISSSITIELDKGLILAIFTK"
     gene            1951..2190
                     /gene="xseB"
                     /locus_tag="PK015_06585"
     CDS             1951..2190
                     /gene="xseB"
                     /locus_tag="PK015_06585"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOL34158.1"
                     /translation="MQAAQVKDEFSLEEDLQKVEEIINKMKSGQLNLEEMIKLYEEGI
                     SLLTRCKQTLEQAELKINQLSATFNQSESEGDNNG"
     gene            2183..3073
                     /locus_tag="PK015_06590"
     CDS             2183..3073
                     /locus_tag="PK015_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOL34159.1"
                     /translation="MDKKVRLKKDMKEKQELVNIILDRYLPRKDEYPKTIHKAMRYSL
                     FAGGKRIRPYLTMSTYEIYRKEVDKIVPVAAAIEMLHTYSLIHDDLPDIDNDDYRRGK
                     KSCHILFGEGQALLAGDALLVSSFDLITLSEIKSEYKVNFVRELAEVAGVNGLIGGQM
                     IDIESEGKKVDQKTINYIHNNKTAKLIQLSVRFGAIAGGAPESELKALEDYGGKMGLA
                     FQIADDLLDIEGTPDELGKSVGKDKIEGKATYPSVFGIAASKSKLQELIKEARESIAP
                     LGEKAELLDALAEYMLTRKS"
     gene            3070..3504
                     /gene="dut"
                     /locus_tag="PK015_06595"
     CDS             3070..3504
                     /gene="dut"
                     /locus_tag="PK015_06595"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOL34160.1"
                     /translation="MNIRYKLLNSEAAPPKKMSSSCSGWDLSAALSEPVILEPGARAA
                     IPTGLALEIPPGYEGQIRPRSGLALNYGLGILNSPGTIDADYRGEIKVILINLSSEAI
                     TINPLMRIAQLVICPVIEVCFEESEQLNDSMRADGGFGHTGY"
     gene            3519..4421
                     /gene="nadA"
                     /locus_tag="PK015_06600"
     CDS             3519..4421
                     /gene="nadA"
                     /locus_tag="PK015_06600"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOL34161.1"
                     /translation="MNIEEKIIKLKNEKNAIILAHNYQIVPIQDLADFRGDSLQLSIQ
                     AKKSQAPLIVFCGVRFMAETAAILNPEAKVILPVEDAGCPLADMINVEQLREFKALHP
                     EAKVVCYINSSAAVKAESDVCCTSANAVQIINSFPEDETILFVPDKNLGSWVAKKSGR
                     KVIVWDGYCPVHQWGFTRRAYEHLKNLYPDYKMIAHPECDEDIIEVADEVLSTSGMER
                     YVKEHDRLIIATDSSFTDYMQHLYPQKKLISLNTRAFCNNMRKTNLKNLLNALEKEET
                     IVEVPPETSEKALSALNKMLELSH"
     gene            4442..5047
                     /locus_tag="PK015_06605"
     CDS             4442..5047
                     /locus_tag="PK015_06605"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOL34162.1"
                     /translation="MDDKGTLIYQDKDAHAITSAPICLKNIALKLFPEYPSQYVLDLG
                     TGCGIIAITLAMERPSWKIEGLDILPHMIELATHNAELCSLNIKFQLANLCTFTTETP
                     YSLIISNPPWQPLGTGKISPHPAKNISRFELMCSMPEILSFLERNLTSEGNALLLYPD
                     FRIPELQTLIYKTSLDIFDLIPCPGIDKHFICHIRYKGTKK"
     gene            5044..5892
                     /locus_tag="PK015_06610"
     CDS             5044..5892
                     /locus_tag="PK015_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34163.1"
                     /translation="MNRSFFFILILITLVLTGWSYVHYQYLESREFRLNQLSELPIFV
                     YMDDLNHLEQLARELLETIPEIDSLFLESGLQAAQEMLSNFPELGIEPNTLTEYHFPN
                     ILTITFKPMLSSFSARDMVLQFLHDNNVPSRDIDDQNPAWSSAKKELDFLNSRWSQTT
                     FFIAITVFLMIVFARLYLYLQEAMHRQGKSQTLLEKVRASEINRLHNALLLTLPILIN
                     IALYYILLALNKLSPLINWSFFLVQLITLVAAIITAILVNNLSSPGKESSKITVAVPV
                     EPKINE"
     gene            5885..6460
                     /gene="pgsA"
                     /locus_tag="PK015_06615"
     CDS             5885..6460
                     /gene="pgsA"
                     /locus_tag="PK015_06615"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOL34164.1"
                     /translation="MNKIPNILTCIRLALVPVFLILTFFCQSENHIIWALIVFIIASI
                     TDFLDGYLARKWKVISDFGKIADPLADKLIVLSALAALTFLPPFKLFVLIFIIIAIRE
                     IGITILREVRKKQGIVMPADKLGKWKTALQMIGIILALFLWAWFPDIASTSIIRLVAN
                     IWFAIVALLTVYSGLNYLQTIQKTSLKGDEQ"
     gene            6457..>6863
                     /locus_tag="PK015_06620"
     CDS             6457..>6863
                     /locus_tag="PK015_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34165.1"
                     /translation="MKKYFYIILILILLLFSLFGCKKQEKSQEPQKNFYTKYQNLIDP
                     SKPIAFGDDNIIYIFAEDNNLKNVKNTLQTSLERTIQVIIEEPYFSTEFKDGSEINDF
                     TNYKNLIYCGTLDGKDRVSTHITSTLAPDYINLV"
BASE COUNT         2192 a         1254 c         1288 g         2129 t
ORIGIN      
        1 taaaaatata taatttaggt ccagtaactc agcggtagag tatctgcctt ttaagcagag
       61 agtcgttggt tcgagtccaa cctggatcac cacagcgacc ccttcgtcta tcggtcagga
      121 cacaagattt tcattcttgt aagaggggtt cgattcccct aggggtcgcc atttttatgt
      181 gtttgagata gttagtcccg ttcgtctagt ggcctaggac tcgtgattct cagtcacgca
      241 acaggggttc gatcccccta cgggacgcca ttcagacttt gacagaaaga gaaaaagaac
      301 tgaaatgaga tgtaattatt tctaacacgg tgatggttaa aatgaaagac gaaaataaaa
      361 tgccggattt ggaaccggag attttcccgg atcggcgtat caaaatcagt tttgaaagtc
      421 tgggtaatct gcacagcttt aaggtgcaac cctggctatt atatgttatt ttactcgtta
      481 tagtaattgg aattatattc ctttttgtcg cttctttccg tggacccgat aaagaaaacc
      541 tcaacaaaat tcaccgctta gaaacagaaa atcaatatct gcaaagtgaa ctggaaaaat
      601 tcaatgtaca actggattca attaacgctt tgcttgattc cctgagggta aaatctgctc
      661 ataaaaaaat caattatcct tcttatagca ataatctgca acccatcaat aaacttttag
      721 tccatcctgc actggatagc aagctttctg caatagaaat caaattagaa gtgataaaaa
      781 atcgcctaac ctatctggat tcaaatggag tttcttctaa agacgatgag ttaagttctg
      841 atgctctaaa aaacataccc tctatccttc ctacctttgg tgaaatatcc agctactggg
      901 gtttgcgtaa tcatcccatt ctgggtggag aaatatggca tcaaggaatt gatattgcca
      961 atcgcactgg aacccccgtt tatgctacag cagaaggtat tgtctcaaga gtggaatctg
     1021 atgatggttg gggtaatgtt atcacaattg accataaagg tggctataga actctttatg
     1081 ggcatcttta taggagtaaa gttcatcttg gtgatattgt gcgtaaggga cagattatcg
     1141 gattgatggg aagcacaggt atgtccacgg gttctcatct acattatgga gtttatctta
     1201 atggagcacc aataaatccc agttattatc tgaatagaca ggacctcagc tattataata
     1261 tggttagaaa atgaagaaag aagcggatat tgcctggctt ttcactacca ccagtcctaa
     1321 agaaatattt tcttcctata agaacataga tttcagcaaa gatttaataa ttgcagttga
     1381 tgcaggatta gaaagggtta ataaattagg tattgagcca gatcttataa taggagatat
     1441 ggatacatta tcacctgaac tattggaaca atatccagac actgaaaaaa ttaaatatga
     1501 tatacaaaaa aatcatactg ataccgagtt agctattctt tggtgtctgg aacataatct
     1561 gaaacaggtt gttatcataa atggattaac caatcgtttt gaccattctc tgggtttgtt
     1621 acaaaatctg gctttactgc atagtaagaa tgtaacggca agaatagaat ccgaaaggca
     1681 ggttgttttt tttctaacaa ataaatcaca tcttaatggg tatgaaggct gtctattatc
     1741 tctctttcct tatggaggtg atgcgactat taagaattcg gaaggattat ttttcccctt
     1801 gaaaaatctg acgctaagtc ctctgcatac cagaggaata agcaataatg tgatttcttc
     1861 ttccataacg atagagcttg acaaaggatt gattctggct atatttacta agtaattaga
     1921 ttattagtca acaagaagag gagaaaatag atgcaagcag ctcaggtaaa agatgaattc
     1981 tcgctggaag aagacttgca aaaagttgaa gaaataataa ataagatgaa aagcggacag
     2041 ttaaatctgg aagaaatgat aaaactttac gaagagggaa tatctctatt gaccagatgt
     2101 aaacaaactc tggaacaagc agaacttaaa attaatcaac tttcggcaac tttcaatcag
     2161 tcagaatctg aaggagataa taatggataa aaaagtgcga ttaaaaaaag atatgaaaga
     2221 aaagcaggaa ctggtcaata tcatcttaga ccgttatctt cctcgtaaag atgaatatcc
     2281 taaaacaatc cataaagcta tgcgatattc tttatttgct ggtgggaaaa gaattcgtcc
     2341 atatctaact atgagcactt atgaaatcta tagaaaagaa gtggacaaaa ttgttcctgt
     2401 ggctgctgct attgagatgt tgcatactta ttctttaatc catgatgacc ttcccgatat
     2461 tgataatgat gactatcgca gaggcaaaaa atcttgtcat attctctttg gtgaaggaca
     2521 ggcacttctg gcaggagatg ctcttctggt tagctctttt gatttaatca ctctatctga
     2581 aattaaatcg gaatataaag tcaactttgt gcgtgaactg gcagaagtcg ctggagtgaa
     2641 tggtttgatt ggtggtcaga tgatagatat tgagtcggaa ggaaagaaag tggatcaaaa
     2701 gactataaac tatatccata ataacaagac agccaagctt attcagcttt ctgttcgttt
     2761 tggagctata gctggaggtg caccagaatc tgagcttaag gctctggaag attatggtgg
     2821 aaaaatgggt ttggcattcc agattgctga tgatttgctg gatattgaag gaacacctga
     2881 tgaattaggc aaaagtgtag gtaaagataa gattgagggt aaggcaacct atccatctgt
     2941 ttttggcata gctgcatcaa aaagtaaatt gcaggaatta attaaagaag cccgggaaag
     3001 tattgcccct cttggtgaaa aagctgaact tttagatgct ttagccgaat atatgctaac
     3061 tcgcaaatca tgaatatccg ttataaactc ctcaattccg aggctgctcc acctaaaaaa
     3121 atgagtagtt cttgctcagg ttgggattta agtgctgctc tttctgaacc tgtaatttta
     3181 gaaccaggtg ccagagcagc cataccaaca ggtttagctc tggaaatacc tccaggttat
     3241 gaaggtcaaa taagacctcg cagcggttta gctttgaatt atggattagg catacttaat
     3301 agtcctggca caatagatgc tgattatcgt ggagaaatca aagttatttt aatcaattta
     3361 tcgtcagaag ctatcaccat taatccttta atgcgtatag ctcaattagt aatctgtcca
     3421 gtaatagagg tttgctttga agagagtgaa caattaaatg atagtatgag ggcagatgga
     3481 ggattcggtc acacagggta ttaataaagg gctgttatat gaatatagaa gaaaaaataa
     3541 taaaacttaa aaatgaaaaa aatgccatta tcttagctca taactatcaa atcgtgccaa
     3601 ttcaggattt ggctgatttt cggggtgatt cattacaact ttccatccag gctaaaaaga
     3661 gtcaggctcc gttaattgta ttttgtggcg ttcgctttat ggcagaaact gccgctattc
     3721 ttaatccaga agcaaaggtc attcttccgg tagaagatgc tggatgtcct ttagctgata
     3781 tgattaatgt ggaacaactc cgagaattca aagctcttca tcctgaagct aaagtagtct
     3841 gctatataaa ttcaagcgca gcagtgaagg cagaaagtga tgtttgttgc acttccgcta
     3901 atgcagttca gattatcaat tccttcccag aagatgagac cattcttttt gttcccgata
     3961 aaaacctcgg cagctgggta gccaaaaaaa gtgggagaaa ggttattgtc tgggatggtt
     4021 attgtcccgt tcatcaatgg ggtttcactc gtagagctta tgaacatttg aaaaacctgt
     4081 atccagatta taagatgata gcacatcctg aatgtgatga agatatcata gaagttgcag
     4141 atgaagtgct ttctaccagc ggtatggaac gatatgttaa agagcacgat agattaatca
     4201 ttgccaccga ttcatctttc acggattata tgcaacatct ctatccacaa aaaaagctta
     4261 tttcgcttaa caccagagct ttttgtaata atatgagaaa aaccaatcta aaaaatctat
     4321 taaacgctct ggaaaaagaa gaaaccattg tagaggttcc tcctgaaact tccgaaaagg
     4381 ctttgtctgc attgaataaa atgctggagc tatcccactg aaaaattgca cccctgttat
     4441 cttggatgat aaaggaaccc ttatttatca agacaaagat gctcatgcta ttaccagtgc
     4501 acctatttgt cttaagaata ttgccttaaa gcttttccct gaatatcctt ctcaatatgt
     4561 tctggattta ggaacaggtt gtggaatcat tgctataact ctggcaatgg aaagaccatc
     4621 ctggaaaata gaaggtctgg atattctgcc tcatatgata gagctggcaa ctcataatgc
     4681 tgaactttgt tccttgaata tcaagttcca gcttgccaat ctttgcactt ttactacgga
     4741 aaccccctat tctttaatta tttctaatcc accctggcaa cctctgggaa ctggaaaaat
     4801 tagcccccat ccagcaaaaa atatcagtcg ctttgaactt atgtgttcta tgccagaaat
     4861 tttatctttt ctggaaagaa atcttacttc tgaaggcaat gctttactgc tctatccaga
     4921 ttttaggatt cctgaactgc aaactctgat ttacaagact tcacttgaca tatttgacct
     4981 tattccatgt cctggcattg ataaacattt tatttgccat atccgttata aaggaactaa
     5041 aaaatgaacc ggagcttttt ctttatcctt atactgataa ctcttgtctt aacaggatgg
     5101 agctatgttc actatcaata tttagaaagt agagaatttc gccttaatca actttctgaa
     5161 ttgcccattt ttgtttatat ggatgacttg aaccacctgg agcaattagc cagagaactc
     5221 ctggaaacca tcccggaaat agattcgtta ttcttagaat ctggattgca agcagctcaa
     5281 gaaatgctct ctaactttcc agaactggga attgaaccta atactttaac tgaatatcat
     5341 ttccccaata ttttaaccat aaccttcaaa cctatgcttt cttccttttc agctcgagat
     5401 atggtattac agtttcttca cgataataat gtcccttcca gagatataga tgaccaaaat
     5461 ccagcctgga gttcagctaa aaaggaatta gatttcttga attctcgctg gagtcaaacc
     5521 accttcttca ttgccattac tgtcttttta atgattgtct ttgccaggct ttatctatac
     5581 cttcaagaag caatgcatcg tcagggtaaa tctcaaactc ttttagaaaa agtgcgggca
     5641 agtgaaatca atcggttgca taatgctctt cttttaactt tacctatttt gataaacatt
     5701 gccttatact atatcttact tgccttaaac aagctctcac ctttgattaa ctggagtttt
     5761 ttcctcgtgc aattgataac tttagttgct gccataataa ctgctattct ggtaaacaat
     5821 ctatcctctc ctggaaagga aagttccaag ataacagtag ctgttccagt tgaaccaaag
     5881 attaatgaat aaaatcccca atatcctgac ctgtataagg ttagcattag ttccagtttt
     5941 ccttatacta accttcttct gtcaaagtga aaatcatatc atctgggctt taattgtctt
     6001 tattatcgct tcaattacag attttctgga tggctatctg gcacggaaat ggaaggtaat
     6061 atccgatttc ggtaaaattg ctgaccccct ggcagataaa cttatagtcc tatctgctct
     6121 tgctgcttta acctttttac caccctttaa attatttgtc ctcattttta tcataattgc
     6181 tatccgggaa ataggtataa ctattctgcg tgaagttaga aaaaaacaag gtattgtaat
     6241 gcctgcagat aaactgggta aatggaaaac tgctcttcaa atgataggga ttatccttgc
     6301 tctttttttg tgggcttggt ttcctgatat tgcatccaca tctatcatcc gacttgttgc
     6361 taatatctgg tttgcaatag ttgctttgct taccgtttat agtggattaa attatctaca
     6421 aaccatacag aagacttctt taaaaggaga tgaacaatga agaaatattt ttatattata
     6481 cttattctaa tcctgctgtt attttctctg tttggttgta aaaaacagga aaaatcgcaa
     6541 gaacctcaga aaaatttcta tactaaatac caaaatttga ttgacccttc caaacccatt
     6601 gcctttggtg atgataatat tatctatatc tttgcggaag ataataatct taaaaatgta
     6661 aaaaatactt tgcagacttc ccttgagcgg actattcaag ttattattga agaaccctat
     6721 ttctctactg aattcaaaga cggttcagaa ataaatgact ttaccaatta caagaatctc
     6781 atctattgcg gaaccttaga tggtaaagat cgggtctcca ctcatataac ttcaactctt
     6841 gctcctgatt atatcaatct ggt
//
LOCUS       DAORZF010000061         6107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_8900_length_6107_cov_314.559980, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000061 DAORZF010000000
VERSION     DAORZF010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 6107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6107
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8900_length_6107_cov_314.559980"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="PK015_06625"
     CDS             <1..650
                     /locus_tag="PK015_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34166.1"
                     /translation="EFSGSVAGGYWLTYARPKFGTNIAVGVTAVMAADEYPYLQTGQF
                     IGLLCGLKGAAEYEKLVDVFAAYRAPGEPLEPYFDEEGNKIVPGRSFSKDILNQPEIK
                     DLIKITPQTEAKFTPEEFELFLQTCPEKDQQLLHQVKQSVDGKIVIDIEKLDKNQFSS
                     LTWEGLNRMTHNTPYKFKVARVGMNAQSVAHIMIIVFIILGNIGYFLQKAQTGKK"
     gene            662..1279
                     /locus_tag="PK015_06630"
     CDS             662..1279
                     /locus_tag="PK015_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34167.1"
                     /translation="MTFDLFSNLVAAFFTLCIFSFLYKDNPFYQMSEQLLVGVSLGYS
                     LVLTYERVFIPYFYQPIFLKHEWILIIPSIIGIFYLFRFSPKLSWLSRYPIAFSMMSV
                     GASVALAMHTSILVQMRQAMVPIENINLALIFIGTITVLLYFFFSKAHTGIYGQFVNV
                     GKWFMMIGFGASFGLTVMARISLLIGRIQFLVNDVFLALKQALFG"
     gene            1372..4260
                     /locus_tag="PK015_06635"
     CDS             1372..4260
                     /locus_tag="PK015_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34168.1"
                     /translation="MKKIYFYLIFILITMMFFFGGCAKETLSEEDHIQDFTVSDVPND
                     DGTGLVLQWHPLDKSNRIIKYNIYRGHRPDSLFLLESLEVDPKVSQPESWLNYTDKDY
                     NATLISFETAPRKLKKEKQQVSSGTLYGNVPRDPKVLAALLPHYDVLALINTPKYYKP
                     SKRIELNEGETKNVYAGYNLNQFETIFANPKAEHSYYYCVVPVNETGRFLPATPILSA
                     IPYNNRPDTTAIFYANYIKDTGEFRFEWSPPSGASNIVSWEAWLMPRYLLPQYYADQK
                     ANAQSPDSIFNANWQKGSIFLKEMQPEYWGQNFYDKTSPEEIKGKLSPDIQLSSYIPV
                     LSYTSYWSSEDGSRVETFQSASLGKSIQIRNSKDLPEVPKYQVLDKPNDKGDNIVLSL
                     GRPFAYVTQATFIDKEHTKFRVNYEISTNSVNDISKLLFRFLDSQGRVLDEVKEGYTD
                     KIIYAHFPKNRNWKEDITVQIYSQLSNEKEFSSHLITQKINYDSKSLRFSGGDIYDGS
                     QNLSILFYDIFRRTPLDSSFYPGMRFRAISRSYDQTVPYPETETVRLLKVDLASQLVL
                     LDPHFTVALDEKTGATFRPSLFKQDTIEYMKALSAQIDSLSRQISSEDTLSETAKNLA
                     ALQEEKNFITSLSSYQQGIKAKNDKEWRKILRKELDLNTRSYEFRLLLTNSRGLWANE
                     DDLPPAQKPQANIKGRTVFLHPEGEWFDTTKYATLIATILMGIFVVIALNQARHKDLY
                     IRPIAGLEELDNAVGRATEMGRPVLFVPGWGSLGDPDTVATMMILNRIARKTAEYDIR
                     LISPQVDYLVLPLAQEMVKNAYDEVGRPDAYNQDDIFFVSDVQFAFSAAVNGIIVREK
                     VATVFYMGYFFAEALLMTETGNQAGAVQIAGTDAITQIPFFITTCDYTLIGEEFYATS
                     AYLSRNPDLVGMLKSEDYFKIILVILAIIGVVLSSLGINTLLQFLPIE"
     gene            complement(4374..5624)
                     /locus_tag="PK015_06640"
     CDS             complement(4374..5624)
                     /locus_tag="PK015_06640"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOL34169.1"
                     /translation="MKKLMLVAILTGGMLILSTLIFAAQLQIGTNSNTTMSNPIYANY
                     PYSYSQQIYTKPQIGAPHPIKITHIRFYYVSGSTLGNSNQWTIYLGHTNKNEFLNTND
                     WIPITGPTFKKVFEGDIASYVPQTNGQWMDIPLSTPFIYDNVNNLVVAVDENTSGSDV
                     ETYWGSFLESFKYNPLRTLYYCGSDDFDPLQPVAGKARTQLVNCIQLIYEENGNPVEL
                     TSFFANISNDYYVTLSWIVQSETEMLGYYIFRNNERNLSTAQIVSPLIDATNTSGTHR
                     YEFVDTELYEVGQYYYWLQYTNLDGSFGFHGPIVVQYNPFNNEMPGLPIITKLGLAYP
                     NPFNPSVNIPFSLGAKTDVQIYIYNSRGQLIKSFNCGTKDIGNYQIMWDGKDRNGNYC
                     DSGIYHIIMKAGTDTYSQKAVLMK"
BASE COUNT         1851 a         1197 c         1032 g         2027 t
ORIGIN      
        1 ttgaattttc cggttccgtg gctggtggtt actggctaac ttatgctcgc cctaaatttg
       61 gcactaatat cgctgtcgga gttaccgcag ttatggcagc tgatgaatat ccctatctcc
      121 agacagggca atttattggt ttactctgcg gactaaaagg tgcggcagaa tatgaaaaat
      181 tggttgatgt ttttgccgct taccgtgctc ccggtgaacc cctggaacca tattttgatg
      241 aggaaggaaa taaaattgtc cctggccgct cttttagtaa agatattctc aatcaacctg
      301 aaattaaaga tttgattaaa atcactcctc aaacagaagc taaatttacc ccggaggaat
      361 ttgaactttt cctgcaaacc tgtcccgaaa aagaccaaca gcttttgcat caagttaagc
      421 aaagcgtaga tggaaaaatc gtgatagata ttgaaaaact tgataaaaat cagttctcat
      481 ccctaacctg ggaagggctt aatcgtatga ctcataacac accatataaa tttaaagtag
      541 ctcgagttgg tatgaacgca caatccgtag ctcatattat gataattgta tttatcatct
      601 taggaaatat aggttatttc cttcagaaag cacaaactgg taagaaataa gggagtatat
      661 tatgactttc gatttattta gtaatctggt agcagctttt ttcaccctct gtatattctc
      721 tttcctgtat aaggataatc cattttatca gatgtccgaa caactcttag tcggagtctc
      781 tcttggttat tcattagttt taacttacga acgggtgttt atcccctatt tctatcaacc
      841 tattttcttg aaacatgaat ggattcttat catcccaagt attattggta tcttctatct
      901 gtttcgtttt tcacctaaac tcagctggct ttcccgttat cctattgctt tttctatgat
      961 gagcgttgga gcctctgttg ctctggctat gcataccagt attctggtac aaatgcgtca
     1021 ggctatggtc cctatagaaa atatcaatct ggcacttatc tttattggaa caattactgt
     1081 cttactctat ttcttctttt ctaaggctca cacagggatt tatggacaat tcgttaatgt
     1141 gggaaaatgg tttatgatga ttggattcgg agcctctttc ggtcttactg ttatggcacg
     1201 tatttctctg ctaataggta gaatccagtt cctggttaat gatgtctttc tggctctaaa
     1261 acaagcccta ttcggctaaa tgctattata gcctgaatat tagttaaagc tctcctccgt
     1321 attatccttc tcccaatgcg gataactgaa gctttgctaa agggttctaa aatgaaaaag
     1381 atatatttct atttaatctt tatcttaatt acaatgatgt tcttttttgg gggttgtgcc
     1441 aaagaaaccc tttcggaaga agaccacatt caggatttta cggtctctga tgttcccaat
     1501 gatgatggca ctggtctggt tcttcaatgg catcccctgg ataaaagtaa tcgtattatt
     1561 aaatacaata tttacagagg gcaccgtcca gatagtcttt ttctcctgga atccctggaa
     1621 gttgatccta aagttagcca acctgagagc tggctaaatt atactgacaa agactataat
     1681 gctactttaa tctcttttga gactgctccc agaaaactga aaaaagaaaa acaacaggtc
     1741 tcctccggta ccctttatgg taatgtgccc agagacccga aagttttggc tgcactttta
     1801 cctcattatg atgttttagc acttatcaat accccaaaat attacaagcc ttctaagcga
     1861 attgaactaa atgaaggcga aacgaaaaat gtatatgcag gttataatct aaatcaattt
     1921 gaaactattt ttgccaatcc taaagcagaa catagctatt attattgtgt tgtcccagtc
     1981 aatgaaactg gtcgttttct tcctgcaact cccattctat ctgctatacc ttataataat
     2041 cgcccggata ccactgcgat tttttatgca aattatataa aagatactgg agaatttcgc
     2101 tttgaatggt ctcctccctc tggagcttca aatattgtct cctgggaagc ctggctaatg
     2161 cccaggtatc ttttacctca atattatgca gatcaaaagg ctaatgccca atctcctgac
     2221 agtattttta atgctaactg gcaaaaaggt tctatctttc ttaaagaaat gcaaccagaa
     2281 tactggggac aaaattttta tgataagact tctcctgaag aaataaaagg gaaattatca
     2341 ccagatattc aactttcctc ctatatacct gttctttcct acaccagtta ctggtcctcg
     2401 gaagatggtt cacgagttga aacttttcaa tctgcttctc tgggaaaatc aattcagatt
     2461 cgtaactcta aggacttacc agaagttcct aaatatcaag ttctggataa acccaatgat
     2521 aagggtgata atatcgtctt atctttaggc agaccttttg cctatgtaac tcaagctact
     2581 tttatagata aagaacatac taaatttcgt gttaattacg agatttccac aaatagcgtc
     2641 aatgatatat ccaaactact atttcgcttt ttggattctc aaggtagagt gctggacgaa
     2701 gtaaaagaag gatataccga taagattata tatgcgcatt ttcccaaaaa tcgtaactgg
     2761 aaagaagata tcacggtcca gatttattca caattatcta acgaaaaaga gttttcctca
     2821 catttaatta ctcaaaaaat aaactacgac tccaaatccc tccgtttttc tggaggagat
     2881 atatatgacg gttcgcaaaa tttaagtatt cttttctatg atatttttcg tcgcactcct
     2941 ctggattctt ctttttatcc gggtatgaga tttcgtgcta tatcacgaag ttatgaccaa
     3001 actgtgccct atcctgaaac tgaaactgtt agacttttaa aagttgattt agcttctcaa
     3061 ttagttttgc tggatccgca ttttaccgtg gctctggatg aaaaaacagg tgctactttt
     3121 agaccttctc tttttaaaca agataccatt gaatatatga aagcgctgtc tgctcagatt
     3181 gatagccttt ctcgccagat ttcttccgaa gatacactat ccgaaactgc taaaaatctt
     3241 gctgctcttc aggaggaaaa aaactttata acctcccttt cttcttatca acagggaatc
     3301 aaagccaaaa atgataaaga atggcgcaag atcctgcgta aagaactgga tttaaacact
     3361 cgatcttatg aatttaggct acttttaacc aattctcgcg gtctctgggc taatgaagac
     3421 gatctacctc cagctcaaaa acctcaagct aatattaagg gaagaacggt tttcctgcat
     3481 cccgaaggag aatggtttga cacgactaag tatgcaactt tgattgctac cattttaatg
     3541 ggtatttttg tcgtcattgc cttaaatcaa gcccggcata aagatctcta tatcagacct
     3601 attgctggct tggaagaact ggacaatgca gtcggtcgtg ccaccgaaat gggacgtcct
     3661 gtattattcg ttccaggatg gggcagtctg ggtgatcccg atactgttgc cactatgatg
     3721 attcttaatc gtatcgcgag aaaaactgct gaatatgaca ttcgtttaat atctccccag
     3781 gtggattatc tggttctgcc tctcgctcaa gaaatggtca aaaatgctta tgatgaagta
     3841 ggacgtcctg atgcctataa ccaggatgat atcttctttg tttcagatgt ccaattcgcc
     3901 tttagtgctg ctgtcaatgg cattattgta cgagaaaaag tggctactgt cttctatatg
     3961 ggttacttct ttgctgaagc tttattaatg actgaaacgg gaaatcaagc aggtgcggtt
     4021 caaattgctg gaactgatgc tatcactcaa attcctttct tcatcactac ctgcgactat
     4081 actttaattg gtgaagaatt ttatgctacc tctgcctatc tctctcgcaa tccagatttg
     4141 gtaggtatgc taaaatcgga agactacttc aagattatcc tggttatcct tgctataatt
     4201 ggagtcgttt tatcctccct cggaatcaat actttgctac aattcctgcc tattgagtaa
     4261 tatatttacc tttttaatga cctgattcgc agaagaataa gcgcttataa cttaaataaa
     4321 aaaacccggt atagaaaata ccgggttttt tgtttattga ttgaaacagt ctatcatttc
     4381 atcagaacag ctttttgact gtaggtatct gttcccgctt tcattataat atgatagatt
     4441 ccactgtcgc aatagttacc atttctgtct ttcccatccc acataatctg ataattacca
     4501 atatccttgg tcccgcaatt aaatgatttt atcagctgac cacgagaatt ataaatatat
     4561 atttgcacat ctgtttttgc gcctaaactg aaaggaatat tgacactcgg attaaaagga
     4621 ttaggataag ctaaacctaa tttcgtgatg ataggtaagc ctggcatttc attgttgaaa
     4681 ggattatatt gaactacaat tggtccatga aatccaaaac taccatccag gtttgtatac
     4741 tgcagccagt aataatattg tcctacttca taaagctcag tatctacaaa ttcatatcta
     4801 tgggttccgg aagtattggt agcatctatt aagggactaa caatttgtgc tgtactcaaa
     4861 tttctttcgt tattacggaa aatatagtac cccaacattt cagtttctga ttgaactatc
     4921 cagcttaatg ttacataata atcattacta atattagcaa aaaaggaagt taattcaacc
     4981 ggattaccat tctcctcgta aattaattga atacagttca ccagttgggt acgagctttt
     5041 cctgcaacag gctgaagagg atcaaaatca tcggaaccac aataatataa tgtccttaga
     5101 ggattatatt taaaagattc aagaaatgat ccccaataag tttctacatc acttccggaa
     5161 gtattttcgt ccacagcaac tactaaatta ttaacattat catatatgaa tggtgtagat
     5221 agagggatat ccatccattg cccattagtt tgtgggacat aacttgcaat atctccttca
     5281 aagactttct taaatgtagg tcccgtaatg ggaatccaat catttgtatt taaaaattca
     5341 tttttattgg tgtgtcccaa atatattgtc cattgattgg aatttcccag cgtagaacca
     5401 ctcacatagt aaaatctgat atgggtaatt ttaatgggat gaggagcccc aatctgtggt
     5461 ttcgtataaa tttgctggct ataactataa ggatagttgg catatattgg gttactcatc
     5521 gtagtattag aatttgtccc aatttgaagc tgtgctgcaa agatgagggt tgataaaatt
     5581 aacatccctc ccgttaaaat agctactaac attaactttt tcataataaa tacactcctt
     5641 taaaaatttt tttgtgctaa attgaaaaaa gtgtttgttt cctctggttt tgctaaagag
     5701 tggataaaat cactcctgca aaaccaactc attggcgata tttgaaattt cgtaggatta
     5761 taattcatta ccccagacct tattaacgat ttttgtaaat acaaatttga tccgtatccc
     5821 cttacataga gatcagaccc cacaattaaa gacattaatt taatacaact catcaatgga
     5881 aaggaatttt gtctcatctc ttcattcctt tttaaaaata acaaccaaaa cattccatct
     5941 ttccattttt tcaactcctt tcatttttgc tctaaatacc gacagccatt taattaaccg
     6001 ttctaaataa aataataatc ctagaaaaaa atctattctt tcttctaatt gttaatataa
     6061 taaatacgat ttaaatgtca agaattattt ttaacttttt tttcatt
//
LOCUS       DAORZF010000062         6094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_8923_length_6094_cov_352.425567, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000062 DAORZF010000000
VERSION     DAORZF010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 6094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6094
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8923_length_6094_cov_352.425567"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..1437
                     /locus_tag="PK015_06645"
     CDS             154..1437
                     /locus_tag="PK015_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34170.1"
                     /translation="MIGISKKITALLITVISLFMISCSTTGHLYKSSLEHYNKGDYEA
                     ALEKGKQILELKPKDKKAQELVQKSYLQLIEETQARINLIEAKAQPDMWDQLVKEYTL
                     LIEYQNRIKPVDFSRLSKEEKAFLSSIQDYTARLAICKYQAARVHFQNGQQLMQSSND
                     PDVQWEAAQEFKIAMEFFPDYPNAANLYALARKNSIKKVVIKEFEDKSGTPKSYGSVG
                     EILSSSIIAKLVQDSLTCEFINIINTNYLNNSNSGEQIITNLPADSIAEISSLAKPNK
                     SPTCNNIIEGKILQINYIAPQTTSVELKDNPNTNLQKSATSKKKSKNKTNEVTCTYQK
                     FIKTSSLKIIATYYIIDAENGIVEYTDSATGYYAWKDEWGKLLSGDEQNLSDNTRMLI
                     QKPEPIPPSELDMLNFAITELSDEIALKLKEYYIK"
     gene            1584..2534
                     /locus_tag="PK015_06650"
     CDS             1584..2534
                     /locus_tag="PK015_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOL34171.1"
                     /translation="MKNILVLGSAGQIGSELVPYLRNIYGNNNVVATYHRQPLIPEIK
                     DGGPCENLDALEPDKVMNAVKKYNIDTIINLVAVLSAVGESKPTLAWKINMDSMLNVL
                     EIAKEAHCAVFIPSSIGAFGPTTPHDNTPQDTIMRPTTIYGISKVAAELLGDYYFLKY
                     GVDARGLRYPGIISNVTLPGGGTTDYAVEIYYEAIKNKSYVCYLKPGTFLDMMYMPDA
                     LRALVDLMEADPSRLKHRNCFNVTAMSFDPEMIAAEIKKHIPEFHMEYQIDSVKQAIA
                     DSWPNKMDDSAAREEWGWKPEYDLASMTEDMLRVLSTKLS"
     gene            2537..3526
                     /locus_tag="PK015_06655"
     CDS             2537..3526
                     /locus_tag="PK015_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOL34172.1"
                     /translation="MLKIGLVGVGHLGRFHAEKLKSNPLCQLVGIYDSNPECCKSVAE
                     EFNLTAFPGYQELLSDVDAVDIATTTSSHYALAKQALEQGKHIFIEKPITSELWQAEE
                     LVNLANQKGLKIQVGHIERFNPVTLKVENLIEDPLFIESHRISSFQPRGTDVPVVLDL
                     MIHDIDLILDFIQSPVKKIYANGVGIFTPSIDIANARIEFENGSIANVTSSRVSLKHE
                     RKIRFFQRDAYISLDFIAKLASVTKINPEMMRFIPLILQGTMDIKPEQLVEIQRFDLS
                     DSEQDALALELNSFIEAIIEDKKPVVDGQAGYRALQVAMQILDCINHQPSTHI"
     gene            3535..4830
                     /gene="asnS"
                     /locus_tag="PK015_06660"
     CDS             3535..4830
                     /gene="asnS"
                     /locus_tag="PK015_06660"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOL34173.1"
                     /translation="MKYILVTEIAEHIGEEIILKGWVRNIRHSGKLLFIIFRDGTGDV
                     QCVAFQPELGEEKFDQAKHLTLESSVIITGLVRPHPKLEGQFEVAVTDLSIIQIAEDY
                     PIGKKEHGADFLLSHRHLWIRSSKQWAILRIRHTVYYAICDFLHQNHFTRFDSPILTP
                     NACEGTTTLFELQYFDEGKAYLSQSGQLYLETGIMSLGRVYDFGPVFRAERSKTRKHL
                     TEFWMMDAEAAFIEHNENIQLQEELIRYVIRTVLKERDNELTILERDKEALKAADAPF
                     GRMSHYEAVEYLRSKGSDITHQSDLGAQDEVLLTENSPVPIFVERWPKEIKAFYMKRD
                     PENPDLVLGSDLIAPEGFGEIIGGSQREDDYELLLQRMKDEKMPIEDYRWFLDLRKYG
                     TVPHSGFGIGLERLVSWISGVHHIRETIPFPRMIYRIYP"
     gene            4928..>6094
                     /locus_tag="PK015_06665"
     CDS             4928..>6094
                     /locus_tag="PK015_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOL34174.1"
                     /translation="MHYPDSFSRITQVMKSSLIRELVASTKNIPDLISFAGGFPSPAT
                     FPTQQLAELFEEVIKNGGSDVLQYGASEGDILLKKQLMQWEGYELKPEEMVITVGATN
                     AIYYYARCLINPGDVILCEAPSFLGTLVTFDAIEADCQSVPINDEGIIIEELEEKVKI
                     LHQQGKNIKFLYIIPDFHNPAGITYSLQRRKEIIQFCIDNNIPILEDNPYSRLRFYGE
                     PVPTFYQVARTDFAPTNIVTEIVSFSKILGPGLRIAYAKGDKELIEKMVSWQQKVTIT
                     PDCVAERAVARFLEKGLMDTHLKYIAEYYRPYLQKMLDELEKQMPPEVQWTKPEGGIF
                     LWLTLPEYINADELFVEASQYKVSFIPGSKFYPEGQEKFNTLRLNFTYSTFEQIE"
BASE COUNT         2004 a         1105 c         1107 g         1878 t
ORIGIN      
        1 aatttataga tatttcaata ggtgttgtat ctttatattc attactatct aaaagaataa
       61 tgaccttcta aaataatttt cccttgacaa tcaatgcctt atctttattt taatataata
      121 tttaggattt taaaggagta tttacctgag ctaatgattg gcatctcaaa gaaaatcact
      181 gccttattga taactgtgat atcattattt atgatttctt gtagtacaac aggacatttg
      241 tataaatctt ctctggaaca ttataataaa ggtgattatg aggcagccct ggaaaaagga
      301 aaacagatac tggaattgaa accgaaagac aaaaaagctc aggaattggt tcaaaaatca
      361 tatctacaac ttattgaaga aacccaggca agaattaatc tgattgaggc taaggctcaa
      421 cctgatatgt gggaccaatt agtgaaagaa tacactcttc tgattgaata tcaaaatagg
      481 ataaaacctg tggatttttc tcgtcttagt aaagaagaaa aggcattttt atccagtatc
      541 caagattata ctgcccgctt agctatatgt aaatatcagg cagcaagagt acattttcaa
      601 aatggacaac agctaatgca aagcagtaac gaccctgatg ttcagtggga agcagcacaa
      661 gaatttaaaa ttgcaatgga atttttccct gattatccta atgctgctaa tctatatgct
      721 ttagcaagaa aaaactctat taaaaaggtg gttataaaag aatttgaaga taaatctgga
      781 acccctaaaa gttatggtag tgtaggtgaa atcctttctt cttctattat cgctaaatta
      841 gtccaagatt ctctaacttg tgagtttatt aatattataa atactaacta tcttaacaac
      901 tcaaattctg gagaacagat aataactaat cttccagctg attcgatagc tgagataagt
      961 tccttggcaa agcctaataa atcgccaact tgtaataata taatagaagg aaaaatcctc
     1021 cagattaatt acattgctcc tcaaaccact tccgttgaat tgaaagataa tcccaataca
     1081 aacctccaaa aatcggcaac ttcaaagaaa aaaagtaaaa ataaaaccaa tgaagttacc
     1141 tgtacctacc aaaaatttat caagacttct tctttaaaaa ttattgctac ttactatatt
     1201 atagatgctg aaaatggtat tgtagaatat accgactctg cgactggtta ttatgcctgg
     1261 aaagatgaat ggggcaaact tttaagcggt gatgagcaaa atctatctga taacacccgg
     1321 atgcttattc aaaaacctga acctattcca ccttccgaac ttgatatgct taattttgca
     1381 attacagaac tcagtgatga gattgcacta aaactgaaag aatactacat taaataacct
     1441 atttcattat tagttaactt attgcaaatt tagccagatt gataaataac ttgctactta
     1501 taaacaacta acagttaaaa gaattgacaa ggctccttga ttcaaaaaaa aggataaaaa
     1561 taatatttta ctaggagaag taaatgaaaa acatcctcgt tctcggttca gctggacaga
     1621 ttggctctga gctggtgcca tatcttcgta acatctatgg aaataataat gttgttgcta
     1681 cctatcatcg tcaacctctt attccagaaa taaaagatgg tggaccctgt gagaatcttg
     1741 acgctctgga acccgacaaa gttatgaatg cggtcaaaaa atataatata gacacaatta
     1801 ttaacttggt agcagttctt tctgcagttg gcgaatccaa accaacctta gcctggaaaa
     1861 tcaatatgga tagtatgtta aatgttctgg aaatagctaa ggaagcacat tgcgctgttt
     1921 tcattccttc ttctattggt gcttttggtc caactacccc tcatgataac actcctcaag
     1981 atactattat gcgtcccaca actatctacg gtatctctaa agttgcggca gaactgttgg
     2041 gagattacta ttttctgaaa tatggagtag atgctcgggg cttaaggtat cccggaatca
     2101 tttctaatgt gactctccct ggtggtggaa caacagatta tgctgtagaa atctactatg
     2161 aagctatcaa aaataaaagt tatgtatgtt atctgaaacc tggcactttt ctggatatga
     2221 tgtatatgcc tgatgcttta agagcacttg tggatttaat ggaagctgat ccttccagat
     2281 tgaaacatcg caattgtttt aatgttacag caatgagttt tgaccctgaa atgatagccg
     2341 cagaaattaa aaaacatatc cctgaatttc atatggaata ccagatagat tctgtaaaac
     2401 aagctattgc cgatagctgg cctaataaaa tggatgatag tgctgccaga gaagaatggg
     2461 gttggaaacc tgaatatgat ttagcttcta tgactgaaga tatgcttcgt gtcctctcta
     2521 ctaaactttc ctgattatgt taaaaattgg cttagtcgga gtaggacatt taggaagatt
     2581 tcatgcagaa aagttgaaat ccaatcctct ttgccaactg gtaggaatct atgattccaa
     2641 tccagagtgt tgtaaatccg ttgcagaaga atttaattta actgcctttc ccggatatca
     2701 ggagctactt agtgatgtgg atgctgttga tattgcaacc accacttctt cgcattatgc
     2761 actggcaaaa caagctcttg aacagggaaa gcatattttc attgaaaaac ctattacttc
     2821 tgaactatgg caagctgaag aactggttaa tttagcaaat caaaaaggct taaagattca
     2881 agtcggacat attgaacgct tcaatcctgt aactctgaag gtagaaaatc tgattgaaga
     2941 cccgcttttt attgaatctc atcgtatatc ttcttttcaa ccacgaggca ctgatgttcc
     3001 tgttgttctg gatttgatga ttcatgatat tgaccttatc cttgacttca tccaaagtcc
     3061 tgtaaaaaag atttatgcta atggagtggg aatctttact cccagtattg atattgccaa
     3121 tgccagaatt gaatttgaaa atggttctat tgctaatgtg acttcatccc gggtctcttt
     3181 gaaacacgaa agaaagattc gctttttcca gcgggatgct tatatttccc tggattttat
     3241 tgctaaatta gcctctgtca ctaaaataaa tcctgaaatg atgagattca tacctttaat
     3301 cttacaaggc actatggaca ttaaaccaga acaattggtt gaaattcagc gttttgacct
     3361 ttctgattct gagcaggatg cacttgcctt ggaactgaac tcattcattg aagcaattat
     3421 tgaagacaaa aaacccgtgg ttgatggaca agctggatat cgtgctttgc aagtagcaat
     3481 gcaaattctt gattgtatta accatcaacc ctcaacgcat atctaaataa aattatgaaa
     3541 tatatcttag ttactgaaat cgctgaacat atcggagagg aaataatcct taaaggatgg
     3601 gtacgtaata tccgtcatag cggaaaactc ctatttatca ttttccggga tggcactgga
     3661 gatgtgcaat gtgtagcttt tcaaccagaa ttgggagagg aaaaatttga ccaagcaaaa
     3721 catcttactc tggaatcttc cgttattatt actggactgg tgagaccaca tccaaagctg
     3781 gaaggacaat ttgaagtagc tgttaccgac ctctctatta ttcaaattgc agaagattat
     3841 cccatcggaa aaaaagaaca cggtgccgat ttcctacttt ctcatcgtca tctctggatt
     3901 cgttcttcca aacaatgggc tatccttcgc atccgtcata ctgtatatta tgctatctgt
     3961 gacttcttgc atcaaaatca ttttactcgt tttgattctc ctattcttac acctaatgcc
     4021 tgtgaaggaa ccactacttt atttgaattg caatactttg atgaagggaa agcctatctt
     4081 tctcaatctg gacaactcta tctggaaaca ggtattatga gtcttggaag agtctatgac
     4141 tttggtccag tttttcgagc agaacgctct aaaacacgta agcatcttac cgaattctgg
     4201 atgatggacg cagaagccgc ttttattgaa cataatgaaa acatacaact tcaagaagag
     4261 cttatccggt atgtaatcag aactgttcta aaagaaagag ataatgaact aaccattttg
     4321 gagcgagaca aggaagccct aaaagcagct gatgcaccct ttggaagaat gtctcattat
     4381 gaagcagtag aatatcttcg ttctaaagga agcgatatca ctcatcaaag tgatttaggt
     4441 gctcaggatg aagttctctt aaccgaaaat tcacctgtac ctatttttgt agagcgctgg
     4501 cccaaagaaa ttaaagcatt ttatatgaaa cgtgaccctg aaaacccaga tttagtgctg
     4561 ggtagtgatt taatcgctcc tgaaggcttc ggagaaataa ttggaggttc acagcgtgag
     4621 gatgattatg agctcttact tcaaagaatg aaagatgaaa agatgcctat tgaagattat
     4681 cgctggtttc tggacctccg taaatatggg acagttccac atagtggttt cggaatagga
     4741 ttggaaagat tggttagctg gattagtgga gtgcatcata tccgcgaaac tattcctttc
     4801 cctaggatga tttatagaat ttatccataa actctctatc tgttagttaa aatattaatc
     4861 ttaagaatag gatttattct tgagaaccga taatcgtgta ttttttaata aaataaatgg
     4921 agaaaacatg cattaccctg attccttttc ccggattact caagtgatga aatcctcttt
     4981 aattcgtgag ttagtggctt ccactaaaaa cattccagat ttaatttctt ttgctggagg
     5041 ttttccttct cctgcaactt ttcctactca acaattagct gaactctttg aagaagtgat
     5101 taaaaatgga ggtagcgatg tcctacaata tggtgcaagt gaaggagata tcttgcttaa
     5161 aaaacaactt atgcagtggg aaggttacga actaaaacct gaagaaatgg tgattaccgt
     5221 cggtgctaca aatgctatct attactatgc cagatgtctg attaatccgg gagatgtcat
     5281 tctctgcgag gcaccttctt ttctgggtac actggtcact tttgatgcca ttgaagctga
     5341 ttgtcagagc gttcctataa atgatgaagg aattattata gaagaattgg aagaaaaagt
     5401 aaaaattctg catcaacaag ggaaaaatat caaattcctc tatattatcc cagattttca
     5461 taatcctgca gggataacct attctctcca aagacgaaaa gagattatcc agttttgtat
     5521 tgataacaat attcctattc tggaagataa tccttattct cgtttgcgct tttatggaga
     5581 acctgttccc accttctatc aagttgccag aactgatttt gctcctacta atatagtcac
     5641 tgaaatagta tctttttcca aaattcttgg tcccggtctt cgtattgcct atgccaaagg
     5701 tgataaagaa cttattgaaa aaatggtctc ttggcaacaa aaagtaacca tcacaccgga
     5761 ttgcgtagct gaaagagcag tagctcgttt tctggaaaaa gggttaatgg atactcatct
     5821 aaagtacatc gctgaatact atcgtcctta tctacaaaaa atgctggatg aactggaaaa
     5881 gcaaatgcca cctgaagtgc aatggactaa accagaagga ggcattttcc tttggcttac
     5941 acttccagaa tatataaatg cagatgaact ctttgtagaa gccagtcaat ataaagtatc
     6001 ttttatccct ggctcaaaat tctatcctga aggtcaggaa aagtttaata ccctgcgttt
     6061 gaactttact tattcaacct ttgaacagat tgaa
//
LOCUS       DAORZF010000063         5972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_9146_length_5972_cov_315.529153, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000063 DAORZF010000000
VERSION     DAORZF010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 5972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5972
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9146_length_5972_cov_315.529153"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(986..2548)
                     /locus_tag="PK015_06670"
     CDS             complement(986..2548)
                     /locus_tag="PK015_06670"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HOL34175.1"
                     /translation="MKPKILLLLTLIVLISSAFATDPWGEPVVLPGSMTVMAQVFING
                     MPASSNDVLAAMVEVAETTELRGKATIQNINGVAGCLIQIFTETNGEIIHFKVWDESA
                     QMVFNASPTLNSEVNGIVGSYPNNMYQIYAGSNMITDPWTEPLILTSSMTVLAQVGIS
                     GNPTENNDILAAFVNVNGQLELRGKQQISIVNGISGCLIQIFTETNGEQVQFKIWDYS
                     EQEIVNCSTTLLTEVQGMIGSWPNDLFPILGGEIYTVENPILFPTGGIYQTPQFVAIT
                     CPTLNAQIYYTLNGTDPTPVSNQYSTPLYFGLNTTTEVRARAYKNYWMPSDVSVHIYT
                     ITGTVPTPIFNPPGGVYNDSIDVVISCNLPVAVIHYTMDGSNPDENSPIFSIPIHLAQ
                     DTLIVIKAKAYKTDWNPSEIASATYDVQVPLSDDITLSLIPCIVGCYPNPFIDSTSIK
                     INLKEHPLNYRVDIYNIKGEKVQQFKGCERGEFEIKWDGKDFKGNKLSSGIYLISLQT
                     KTFSDIRKIILK"
     gene            complement(2561..5908)
                     /locus_tag="PK015_06675"
     CDS             complement(2561..5908)
                     /locus_tag="PK015_06675"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOL34176.1"
                     /translation="MNSRILVLLLCIIALATILPAKEMAPYSYPNKIEIEPQTKVYSS
                     SRIAPEYTFTRTPISIMTSYYDYMIGSYHSLPLQIIPDNAEGGYFMTYHGRRMASGTR
                     RVFYSYISPQGEIIANNEISDQNLNEGYPTLAVDPVSGKPLYAWHGNFDADLEYEIKF
                     ASDAFLDGITGLFHYQIIFDNPIIIDPPDGPITYDNDFDWPTAVIGPSPIAGKRRIYV
                     VATNSVTHSSYPVENPYIAYADFNEEDIEEGNDLVWSYTSIPELNQWNVDMNWRRPFH
                     SITCDNSGNLYYAGYHTAYENDGITPINEPDLDVFICPNYGQGTWRRVSSSSHLNMWN
                     PPTSPTDNTGYFVSPVTGQPYGDNGDIYFGIHNSGHLNASVDNEGRVHVFAIWAFKNS
                     DNYYYPNYQYVKEFIFNPQTETFTIKDICPQKDPADTFNTTFCPWDLEPPFGEVDEFS
                     NIAPSNPLIVSDWPYPHWDDTAHSDAMQFHYNNCKITNANEEGMMAAVWQNSERARRF
                     NKFGDMQYLAYANTPEIWIAVSPDNGNTWSEPIILNNVDTAQFAGLKPMWVYPANKIK
                     YIGMQEGHKVGKLALMFYNDYTWGSNVITPPYHMNPDGGQVMFTELQIVFPVQLPTED
                     PFGEPIVLSSSMIVMAGVQINGVMASEGDVVAAYVNVNGVPQLRGKETVQVIDGVAGC
                     LLQVYTETNGEIVYFKVWDASTNRVYFAEETLNSIVNGTIGEYPQNLFWLNFGTSQLQ
                     IINLQTGWNMISLNVHPESMDITDIFNDIMSNIVMIKSPNGVFIPDNPYNTLTNLADG
                     KGYFVKVSSPCNLLVNGIAIDVAQPINLNPGWNLIGYTPQVTLNTGAALANIATHLIQ
                     IKGEEGVYETNNPFSNLNVLHPGKAYWIKVNASCNLVYPLSGKQNIIFQPVLSCKKYG
                     TPIVKTNSQTVLCSLNELAQEGDILATLVNEELRGLSYIKKNNGITGALINIFTEEVG
                     EIIQFKLIKKDSDEVYNLLPDLLSAPGEAIGNYEQGIFYNLTPDQNQIPEINTALISA
                     YPNPFTNSTSISLIMGKEKSPIQLNIYNLRGQKVKTLFDGTLDSGNHNLIWDATDDNG
                     RKVASGIYYCKLHSAGKNQLLKLLLLK"
BASE COUNT         1952 a         1057 c         1104 g         1859 t
ORIGIN      
        1 tgagggggtg gggggcatat tttttgacct tatttaattg attgatggat agtgaaatac
       61 gatttggaca gtgattttgt cctgaaatgg acttaaatcc ttcattttta ataagttgtc
      121 ccaatattgc tttgctctga caaatcacag tccaaaactc catttttaat cttctaattt
      181 ccttaaacta aatctaattt tagttgtcaa tgattttttt ctgcaagtct aacaattttc
      241 gtgttcaatt gacaatgagg tagataatat aatcaattaa attgcataat tcttttagaa
      301 ggtgataaat tgacagaaga aaaggtgaaa gtattgacct gtatggattt gattaaatat
      361 aaaatttcca atctcctatc tgtctctctc ttagcacctt acaaagaaaa gagctaaggg
      421 agagccaaga tagtttaagg cattaatcaa attgccaaag atgagcggaa aaatggacaa
      481 aaatacaatg cagtttttta ataggcaaat aaaaataaat tcattagaaa cgaatattaa
      541 gactaccaat agcaattaag agatgcagaa agaaaagttt aaagctcaga atgataaatt
      601 gttattagca agcaaactat caaatatatt actttaaaaa gtggtttaaa taattttgcg
      661 ctcagaatga taacaactgg agcttattat ttattttttc ttgttttaaa gtaattttta
      721 ttgatgccat attttcttag tgatgtgcaa tctttataaa gcattataat tcaataaaca
      781 gcaagaaaga gatatatttc cccaattacc agggagctaa tggttaacaa ttgctataga
      841 gataagtttc tatttgacta tctaatatag catagaaaat cctgatagtt ttgccaaacg
      901 agtaagtatt acctccttgg cgaaaatcct aatagttatg taatacaagg agttctcgca
      961 ccccttaatg tatgtttgct ataggttatt tcagtataat tttccggata tcgctaaaag
     1021 ttttggtctg cagggaaatt aaataaattc cggagcttag tttatttcct ttaaaatctt
     1081 taccatccca tttaatttca aattcacctc gttcacatcc cttaaattgt tgcacttttt
     1141 ctcctttgat attataaata tccacacgat agttcagagg gtgttctttg aggttaattt
     1201 taatactggt ggaatcaata aaaggattgg ggtaacaacc aacgatacaa ggaattaaag
     1261 aaagggtaat atcatcggac agagggactt gcacatcata ggtagcagaa gcaatttcac
     1321 tgggattcca gtcagtttta taggctttag ctttaatgac aatgagcgta tcctgagcca
     1381 gatgaatagg gatagaaaaa atgggagaat tttcatcagg atttgagcca tccatagtat
     1441 aatgaatcac tgctactggc aggttacaac ttatcaccac atcaatagaa tcattataaa
     1501 ctccacctgg tggattaaag ataggagtag gaacagttcc ggtgatagtg tagatatgaa
     1561 ctgaaacatc gctgggcatc caataatttt tataggcacg agcacgcact tctgtggtag
     1621 tgttcaagcc aaaataaaga ggagttgaat attgattaga gactggagtg ggatcagttc
     1681 catttaaagt ataatatatt tgtgcattta aggtgggaca agttatagca acaaattgag
     1741 gagtttgata gattccacca gtagggaaca atataggatt ttccacggta taaatttcac
     1801 ctcccagaat tggaaagagg tcattaggcc aggaacctat cataccttga acctcagtaa
     1861 gaagtgtagt ggagcaattt acaatttctt gttcactgta atcccatatt ttaaactgta
     1921 cctgctcacc attggtttcg gtgaaaatct ggataagaca tccagaaatg ccattaacaa
     1981 tagagatttg ttgttttccg cgtaattcta gttgaccgtt aacattgaca aaagcagcaa
     2041 gaatatcatt attttccgta ggattaccgg agattccgac ttgcgccagg acggtcatac
     2101 tgctggttaa aattaaaggt tctgtccagg ggtcagtaat catatttgag ccagcatata
     2161 tttgatacat attattggga tagctaccaa ctataccatt aacttcggag ttaagagtcg
     2221 gagaagcatt aaagaccatt tgagcacttt cgtcccagac tttaaagtga ataatttccc
     2281 cgttggtttc ggtaaaaatt tggattagac atccagcaac tccattgata ttttgaatag
     2341 tggctttacc acgaagttcg gtagtctctg caacctctac catagctgcc aacacatcat
     2401 tacttgaagc tggcattcca tttataaaaa cctgagccat aacagtcata cttcctggca
     2461 ggacaacagg ttctccccag ggatcggtag caaaagcaga acttattagc acaatcaagg
     2521 tgagtagaag gagaatttta ggtttcataa cacactccaa ttacttaagt agcagaagtt
     2581 tgagcagttg atttttaccg gctgagtgca atttacaata atagatgcca gaagctactt
     2641 ttctgccatt atcatctgtt gcatcccaga tgagattatg atttccagag tcaagagtcc
     2701 catcaaaaag agtttttact ttttgacccc ggaggttata gatatttagc tgaatcgggg
     2761 atttttcttt ccccataatc aaagaaatag aagtggaatt tgtgaaagga ttaggataag
     2821 cagaaataag agcagtatta atttccggga tttgattctg atcaggagta agattataga
     2881 aaatcccctg ttcataattt cctatcgctt caccaggtgc acttaataaa tctggcagaa
     2941 ggttataaac ttcatcgcta tcttttttaa tgagtttaaa ctggattatt tcaccaactt
     3001 cttcagtaaa gatattgata agagcaccag taatgccatt atttttcttg atataggata
     3061 atcctcgtaa ttcttcattc accagagttg ctaaaatatc accttcctga gcaagttcat
     3121 tcagtgaaca aagcacagtt tggctattgg tcttcacaat gggagtccca tattttttac
     3181 aggaaagaac aggctgaaag attatatttt gtttcccaga gagaggataa actaaattgc
     3241 aagaggcatt aactttaatc cagtaggctt ttccaggatg gagaacattt agattgctga
     3301 aaggattatt ggtttcatac acgccttcct cacctttaat ctggatgaga tgagtagcaa
     3361 tattagctaa ggcagcacct gtattcaaag taacttgagg ggtataaccg attagattcc
     3421 aaccaggatt taaattaata ggttgagcaa catcaattgc aattccgtta acaagcaagt
     3481 tacagggact ggaaaccttg acaaaatagc ctttcccgtc ggctaaattg gttaatgtat
     3541 tataaggatt atcaggaata aaaactccat tgggtgattt aatcattact atattggaca
     3601 taatatcgtt aaagatatca gttatatcca tactttcagg atgtacatta agagaaatca
     3661 tattccagcc agtctgtaga ttaataatct gaagctgaga ggttccaaaa tttagccaga
     3721 aaaggttctg gggatattct ccaatagttc cattgactat actattgaga gtttcttccg
     3781 caaaatacac tctattagta ctggcatccc agaccttgaa atagacgatt tctccattag
     3841 tctctgtata aacctgcaaa agacaaccgg caactccatc tatgacctgt acggtttcct
     3901 tcccgcgcaa ttgaggaacc ccattgacat tgacataagc tgccactaca tctccttcag
     3961 aagccataac tccattaatc tggactcctg ccattactat catactgcta cttaggacaa
     4021 ttggctctcc aaaaggatct tctgtaggta gttgaacagg aaatacaatc tggagttcag
     4081 taaacataac ctgtccacca tcagggttca tatgataggg tggagtaatt acattggaac
     4141 cccaggtgta atcgttatag aacatcagag ctaattttcc aaccttatgt ccttcttgca
     4201 tacctatata tttaatctta tttgcaggat agacccacat aggtttgaga ccagcaaatt
     4261 gagcagtgtc tacattattc aggataatag gttcactcca ggtgttgcca ttatctggag
     4321 agacagcaat ccaaatttct ggagtatttg cataagctaa atattgcata tccccaaatt
     4381 tattaaacct gcgagctctc tcagaatttt gccaaactgc tgccatcatt ccttcttcat
     4441 tggcgttggt gattttgcaa ttattataat ggaactgcat agcatcacta tgagctgtat
     4501 catcccaatg gggatagggc caatcgctca ctattaaagg atttgaagga gctatattac
     4561 taaattcatc cacttctcca aaaggaggtt caaggtccca gggacaaaaa gtagtattga
     4621 aggtatcggc tgggtctttc tggggacaaa tgtctttaat ggtgaaggtt tcagtttgag
     4681 gattaaaaat aaattcttta acatattgat aattagggta ataataatta tcactatttt
     4741 taaatgccca aatagcaaag acatgaactc ttccttcatt atctacagag gcatttaaat
     4801 gaccggagtt atgaatgcca aaatagatat ccccattatc accataaggt tgaccggtaa
     4861 cgggagaaac aaaatatcca gtgttatcag taggtgaagt tggaggattc cacatattca
     4921 gatgactgga actactaact cttctccagg tgccctgccc ataattagga cagataaaca
     4981 catccaaatc aggttcatta ataggggtaa ttccatcatt ttcataagct gtatgatagc
     5041 cagcataata gaggttaccc gaattatcac aagtaataga atgaaaaggt cttcgccagt
     5101 tcatatctac attccattga tttagttccg gaatggaggt gtaactccag accaagtcat
     5161 tgccctcttc aatatcttct tcattgaaat cagcataagc aatataggga ttttcaacag
     5221 gatatgaact atgagtaact gaatttgtgg caacgacata gattcttctt ttacctgcta
     5281 ttggtgaagg accaattacc gcggtaggcc aatcaaaatc gttatcataa gttataggac
     5341 catcaggagg atcaattata ataggattat caaaaataat ttgataatga aaaagaccag
     5401 taataccatc caggaacgca tctgaggcaa actttatctc atactcaaga tcggcatcaa
     5461 aattaccatg ccaggcatat aatggtttac cggaaacggg atcaaccgct aaagtgggat
     5521 agccttcatt taaattttga tcactaatct cattattagc tataatttcg ccttgtggac
     5581 taatatagga ataaaaaacc cgacgagttc cacttgccat tcttcttccg tgataggtca
     5641 taaaatagcc gccctctgca ttatcaggga taatttgtaa gggtaaactg tgatagctac
     5701 caatcatata gtcataataa gaagtcatta ttgaaattgg tgtcctggtg aatgtatatt
     5761 ctggagcaat tctacttgag gaataaactt ttgtctgtgg ttctatctca attttattgg
     5821 gataggaata aggagccatc tccttagctg gtaatatagt agctaaggct atgatacata
     5881 gtaataagac taaaattctg gaattcattt ttcctccttt aaatccttat ttagtcatta
     5941 acattttaat atgtttcttg taatgaggtg tt
//
LOCUS       DAORZF010000064         5286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_10641_length_5286_cov_390.548461, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000064 DAORZF010000000
VERSION     DAORZF010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 5286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5286
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10641_length_5286_cov_390.548461"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..962)
                     /locus_tag="PK015_06680"
     CDS             complement(69..962)
                     /locus_tag="PK015_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOL34177.1"
                     /translation="MDLKSFNFLYGKVHKASKEEIMKELHLSEEEYQALEESLSETLQ
                     QIIEEKDKAKTIGPDFVRLTSYLYEDISDQQKGLPHPPAIKPRTGEKITLPAPETLTM
                     PEISLAQAINQRKSLRKYSPQPLSLQELSFLLWATCWVKEFHSTKYNEFTRRNVPSAG
                     SRHPMECYLLINKVESVPPGLYYYHPLEHALIKLQTSSDIAKEIYEACLEQSMVIEDA
                     VTFIFTAVPYRASWRYQQRAYRYLYVDVGHIGQNVHLAAEAIQAGACMIGAFVDEAMN
                     HCIGLDEKEEFVIYISAVGKK"
     gene            complement(978..2072)
                     /locus_tag="PK015_06685"
     CDS             complement(978..2072)
                     /locus_tag="PK015_06685"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOL34178.1"
                     /translation="MKKRLLIATGGGDCPGLNAVIRAIVKRAQQEPEWEILGSIDAFN
                     GILMDPIHLVELTPEKVAGIHVQGGTIIGTTNQGGPFSWPVKNPDGSWTVIDRSADFM
                     ERIRNENIEAIINIGGDGSQRISQGLFELGCHIIGVPKTIDNDLSATDFTFGFQTAVD
                     IATDAVDKLVTTAASHHRILILEVMGRYAGWIALHASIAGGAEVCLIPEIDYDINSVI
                     EALKRRTASGKEFANIVIAEGAKPKGGSLSYLEEDNPCGAKRLYGAGLRLEQELKDAG
                     CEIEIRTAILGHLQRGGRPNAFDRILASQFGVKAFELVLQEKWGYMVSYRHPNIVAVP
                     IIDAIKEYNYVSPDSDLVNTARGLGICLGD"
     gene            complement(2069..3235)
                     /gene="dxr"
                     /locus_tag="PK015_06690"
     CDS             complement(2069..3235)
                     /gene="dxr"
                     /locus_tag="PK015_06690"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOL34179.1"
                     /translation="MVKLALLGATGSVGSSTIEVVKEQNEHFVITLASAHKNWYKLCQ
                     IAGELSIPKVVLTGIEDTAQQSFIRNQYPNLKVYFGINELLNLLQDEDYDLALNAISG
                     SAGLRSSFAIAQRGKNLALANKESLVMAGHLLMPLIKSQGIKILPVDSEHSAIFQLLE
                     GHPHKEIRKIYITASGGALRDVPLDELENITLERALQHPNWSMGTKVTLDSATMFNKA
                     LEVMEAHWLFDLDFERIDCVLQPQSVVHSLVEFIDGAIFAQLSQPDMKLPILYALSYP
                     ERLPSKLVQTDLKHLGNLEFSPIPTERYPLFILGLQVAYAGGILPTVLNSANEAALRL
                     FQAGKIRYADIYRICQEAVQNCINEPEPSLEQILTVNEQVCQRILRQYNFGDIQ"
     gene            complement(3229..4182)
                     /locus_tag="PK015_06695"
     CDS             complement(3229..4182)
                     /locus_tag="PK015_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34180.1"
                     /translation="MKKLIIITLFMVAMISLNAEKYAGEIFQISPGVQNQAMGNTGLT
                     YSSSLAEGWWNPALLAISDDNGVEVMRVEHFEGLMQQNQFSVIFGSKTRTSLQLNHLG
                     IDKIKLTELENPDEELSNDNRPQIWKTVSNNDLIFTLGLGRSIKNNLHLGLSPKLAYR
                     SLAENNGFGIGADLGMLWEPLNGFKVGANLRDCFSTQIIWENGTYEIALPNLDVEAGY
                     DFSVFSHKIPFHTALRTQIYAEDRGDASTVSAGPMSADFHFGMMAQPIPTLKVMAGWD
                     IDSFTAGLGINYKNIGIDYAWVNGSPDALGSSQRLSLNYKW"
     gene            complement(4298..5242)
                     /locus_tag="PK015_06700"
     CDS             complement(4298..5242)
                     /locus_tag="PK015_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34181.1"
                     /translation="MRDNGIKPIPRKKTGIDKTEAIIWYICVFFIVLGGIFHCTKHLY
                     LDNPFIPEVFRQLYFQSESLDWAQTVMAIVTLSMFFLAISFSKNMEKKVPAYIRLLVI
                     AFALIAMIEATFNSAWDIFANSLLGIVILILTFVPPPQRKVYFPPEFMIIIYLFIFAS
                     MFCGEVLHFYYLFSIWDIIVHFTSAPFIGYAGFLMVYTKNKDVNIHHKISPLFIVLYA
                     FCFSMMIGVVWEFFEYGVDVILGSNMQKARNLELVYGYFDTRLGLLDTMEDLIVDALG
                     AFLVSGICYYYLTTIKERGNNFFHMRDVFIEENPELFQ"
BASE COUNT         1632 a         1152 c          938 g         1564 t
ORIGIN      
        1 gagcttcata aacggggacg tttatgaccc catatacgga acttgaaacg ataaggacat
       61 cgtttcacct atttttttcc taccgctgaa atgtatatta caaattcttc tttttcatct
      121 aaacctatac agtggttcat tgcctcatcc acaaaagctc ctatcataca ggcacctgct
      181 tgaattgctt ctgcagcaag atggacattt tgtcctatat gtccaacatc aacatacaga
      241 tagcgatagg ctctttgctg atatctccag ctggcacgat aaggaactgc ggtaaaaata
      301 aaagttacgg catcttctat gaccattgat tgttccaggc aagcttcata tatctcttta
      361 gcaatatcag atgaagtctg gagtttaatc agagcgtgtt ccagtggatg atagtaatat
      421 agaccaggtg gcacactttc aactttattt ataagaagat agcattccat tggatgacgg
      481 cttcctgcag aagggacatt gcgtctggta aactcattgt atttagtgct atggaattct
      541 tttacccagc aggtagccca gagcaaaaat gatagttcct gcagagaaag gggttgaggg
      601 gaatattttc gtagactttt tctctgattt atagcttgag caagggagat ttctggcata
      661 gttaaagttt cgggagcagg aagcgtaatt ttttcaccag ttcttggttt gatagctggc
      721 ggatgaggca aacccttttg ctgatcactg atatcctcat aaagatagct ggtcaagcga
      781 acaaaatccg gtcctattgt ttttgcttta tctttttctt caataatctg ttgtagcgtt
      841 tcggataaac tttcttccag cgcttgatat tcttcttcgg aaagatgaag ttccttcatt
      901 atctcttctt tagaagcttt atgaaccttc ccatagagga aattgaagct tttcaaatcc
      961 atcgtttctc ctttatttca atctcccaga cagattccta aaccacgtgc agtattcacc
     1021 aaatcggaat cgggactcac atagttatat tcttttattg cgtctataat aggcacagcc
     1081 acgatattag gatgtctata ggaaaccata tatccccatt tttcttgtag aaccagctca
     1141 aaagctttaa ctccaaattg agaagcaaga atgcggtcaa aagcattagg acgtccacct
     1201 ctttgcagat gaccaaggat agcagtcctg atttcaatct cgcatcccgc atctttcaat
     1261 tcctgttcca gccttaatcc tgcaccataa agtcttttcg ctccgcaagg attatcttct
     1321 tccagataac ttaaagagcc acctttaggt tttgctcctt ctgctatgac gatattggca
     1381 aactctttcc cggaagctgt acgacgcttt agtgcttcta tcacggaatt gatatcgtaa
     1441 tcaatctctg gaataaggca aacttcagca ccacctgcaa tagatgcatg aagcgctatc
     1501 caaccagcat agcgtcccat aacttctaaa atcaagattc tatggtgact ggctgcagtg
     1561 gtaacaagtt tatccacggc atcagtggca atatctacag cagtttgaaa tccgaaagta
     1621 aaatccgtag cagaaaggtc attatcaata gttttgggca ctccaatgat atgacatccc
     1681 agttcaaaca atccttgaga gattctttgg gaaccatctc ctccgatatt aatgattgcc
     1741 tcaatatttt cattgcggat tctttccata aaatcagcgg aacggtctat taccgtccag
     1801 cttccatctg gatttttcac aggccaagaa aaaggaccac cttgattggt agtgccgata
     1861 atagttccac cttgaacatg aatcccggca actttttctg gagttagttc taccagatga
     1921 atagggtcca tcaggatgcc attgaaagca tcaatacttc ccaggatttc ccattccggt
     1981 tcctgttgtg ctcttttcac tatagcacgg ataaccgcat ttaatccagg acaatcccct
     2041 cctcctgttg caattaaaag tctttttttc attgtatatc tccaaaattg tattgtctca
     2101 agatacgctg acagacctgc tcattgaccg ttaatatctg ctccaacgaa ggttctggct
     2161 cattgataca attttgcact gcttcctgac aaattcggta tatatcagca tatctaattt
     2221 tccccgcttg aaaaagacgt aaagctgctt cattggcact atttagcacg gttggtaaaa
     2281 ttcctcctgc ataagctacc tgaagtccaa gaataaaaag aggatagcgt tcggtgggaa
     2341 taggagaaaa ctccagattc cccagatgtt tcaaatcggt ctgcaccaat ttggaaggta
     2401 aacgctcagg ataggataga gcatagagaa tgggtaattt catatctggt tgacttaatt
     2461 gtgcaaaaat ggcaccgtca atgaattcta ccagtgaatg gaccacagat tgaggctgaa
     2521 gcacacaatc tatccgttca aaatccaggt caaaaagcca gtgtgcttcc atcacttcca
     2581 gtgctttatt gaacattgtc gctgaatcaa gcgttacttt tgtccccatt gaccagttag
     2641 gatgttgcaa tgccctttct aaggttatat tttccaactc atctaaagga acatcgcgaa
     2701 gagcacctcc tgaagcagtg atgtaaatct ttcgtatttc cttatgagga tgcccttcta
     2761 atagttgaaa tattgcacta tgttcactat ctaccggcag tattttaatc ccttgtgact
     2821 ttataagagg catcaaaaga tgtcctgcca taaccagtga ttccttattt gccagagcca
     2881 gatttttccc gcgttgagcg atggcaaagc tgcttcttaa acctgcagag ccacttatag
     2941 cattcaaagc aaggtcataa tcctcatctt gaagcaagtt tagcagctca tttatcccga
     3001 aatatacctt caaattagga tattgattgc ggatgaatga ttgctgagca gtatcttcta
     3061 taccggtgag aacaacttta gggatggaaa gttcacccgc tatctgacag agcttatacc
     3121 agtttttatg cgcagaagca agggtgataa caaaatgttc attctgctct tttactactt
     3181 caatggtgga ggagccgacg gaacctgttg ctccaaggag agcaagcttt accatttata
     3241 gttcaggctc aacctttgcg aggaacctaa ggcatcaggt gagccgttaa cccaggcata
     3301 gtctattccg atatttttat agttaattcc cagaccagcg gtaaaactat caatatccca
     3361 tcctgccata actttcagcg taggaatagg ttgtgccatc attccaaaat gaaaatctgc
     3421 actcataggt cctgcagaaa cagtggaggc atcacctctg tcttcagcat atatttgagt
     3481 gcgtaatgcc gtgtgaaaag gaatcttatg cgaaaaaaca gaaaaatcat aacccgcttc
     3541 cacatccaga ttgggcaagg ctatctcata ggttccattc tcccatatta tctgagtgga
     3601 aaaacaatct ctgaggttag caccaacctt gaaaccattt aagggttccc agagcattcc
     3661 caaatcggct ccgatgccaa aaccattatt ttctgctaaa ctacgataag ccagcttggg
     3721 actgagtcct aaatgtagat tattctttat agagcgccca agaccgaggg tgaaaatcag
     3781 gtcattattg ctgactgttt tccagatctg aggacgattg tcattactta attcttcatc
     3841 tggattttcc agttccgtaa gttttatctt atctattccc agatgattaa gttgcaaaga
     3901 ggtccgggtc ttagaaccaa aaataacaga aaactggttt tgctgcatca gaccctcaaa
     3961 atgctctact ctcatcactt caactccatt atcatcagag atagcaagaa gagcaggatt
     4021 ccaccatcct tcagctaatg aggaactata agtcaggcca gtatttccca tagcttgatt
     4081 ttgcacacct ggactaatct gaaatatctc tcctgcatat ttttctgcat ttagagatat
     4141 catagctacc ataaatagcg taataataat gagcttcttc ataattttaa cctaagtgtc
     4201 aatatctaaa aagaaagaat ttagtaaagc tttttatgct cttctaaaat ttaaaaattg
     4261 atcagactga actggaagaa ttccccagca acaatgttca ctggaaaagc tcaggatttt
     4321 cctctataaa tacatccctc atatggaaga agttatttcc tcgttccttt atagtagtca
     4381 aataataata acaaattcca ctgaccagaa aagctcccaa ggcatctaca atcaaatcct
     4441 ccatcgtatc cagcaaaccc agacgagtat caaaataacc ataaaccagc tccagatttc
     4501 ttgccttttg catattacta cccaaaatta catccactcc atattcaaaa aattcccaaa
     4561 ctacacctat catcatagaa aaacaaaaag cgtagagaac aatgaaaaga ggactgattt
     4621 tatggtgaat attgacatct ttgttcttcg tatagaccat caaaaagcca gcataaccaa
     4681 taaaaggtgc tgaagtgaaa tgaacaataa tatcccagat actgaataaa taataaaaat
     4741 gtaaaacttc accacaaaac attgaagcaa agataaatag ataaatgata atcataaact
     4801 cgggtggaaa ataaaccttc ctctgcggtg gaggtacaaa tgttaaaatc aaaatcacta
     4861 tacccagcaa agaattggca aaaatatccc aggcagaatt aaatgtcgct tctatcatag
     4921 ctatcagggc aaatgctata accagcaaac gaatataagc aggaaccttt ttttccatat
     4981 tcttggaaaa actgatagcc aaaaaaaaca ttgaaagcgt cactatcgcc attactgttt
     5041 gtgcccaatc taaagattct gattgaaaat aaagttgacg aaaaacctca ggaataaagg
     5101 gattatctaa ataaaggtgc tttgtacaat gaaatattcc ccccagaact atgaaaaaca
     5161 cgcagatata ccagattatg gcttctgttt tatcaattcc ggttttcttc cttggaatcg
     5221 gctttattcc attatcccgc attttacttt ttcactcttc ctcagtttca aaattataaa
     5281 ttttac
//
LOCUS       DAORZF010000065         4692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_12382_length_4692_cov_349.427216, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000065 DAORZF010000000
VERSION     DAORZF010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4692
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12382_length_4692_cov_349.427216"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..242
                     /locus_tag="PK015_06705"
     tRNA            165..242
                     /locus_tag="PK015_06705"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:200..202,aa:Val,seq:gac)
     gene            254..330
                     /locus_tag="PK015_06710"
     tRNA            254..330
                     /locus_tag="PK015_06710"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:288..290,aa:Val,seq:cac)
     gene            462..3506
                     /locus_tag="PK015_06715"
     CDS             462..3506
                     /locus_tag="PK015_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34182.1"
                     /translation="MKLKYYASSLLLLFLIFFGCSSQKKADNSNTGNFPVENLQIGDI
                     PNDGGDGLFLSWKPLPKEKRVQEYRVYRGIHPDTLFFLASVQVNVKTGVAADTMFFYD
                     SGTSDFLSIESPKKLKYEKGAKGSNLYRGIPRDPEIAARLSESFDLISIMDDSDYYYH
                     SKKSYSADEKDKDKDKDVYAGVRLDQQRIVAALKSTPLGQTPQYKYYYTVVPVDERNQ
                     ILGIAEPVSASPIDDIPQTSPALYSVAVQDKKEMQLEWDYPINSDDLMMYLIYAVPGI
                     TKDLWKTLTPQEKEQITSTRGILVAQGLVGGGALKNNCIIKEADFKAAGLNWEQAYQT
                     LYTLKFVDGSNNISPVSEASLPKVINSSQLPSAPKYRVEDKPMDKGDRLTLTWQEPIV
                     FLTRTTSHKKDGSRLKVNYQINKTDAQDIQNIYFDFYEPGSDIPFAQINEFHQDNIIY
                     VDIPQKYSLRNGGKLPTDSLKVEITINSRPYSIDPKTGRILHDKARIIPDYKIIQYLK
                     PDPAMLAYMPTNSFIVNGHNVSTIKNVVYRKGYRSSNFTKIKSNTCYENFLDVSVGYI
                     SSITKPILGFNFVKDGKLYTYIDGKRYVRNLQPGEKASSLALLPSTIDFTYDPVNKTT
                     LNISIYLDEAQKKLTKLSDDIKESQQKLAAYKDSLTAATPAMAILYQENINRLEQEIN
                     TKESQLKIYQENPYFQEALKARNSHQMMRYVASIREPELRKYTYSIVRTNEKGFFAET
                     PPDVNKEGEFNYYTPISNWFDWTKLVTLIAVFLFGIDVVIFINLAKRGKNLYLRPLAG
                     LQEIDNAVGRATEMGRPILYCMGIGGLSDVATIASMGILSQVAKKAAEYDTRLIVPCY
                     DYLVMPIAQEIVQEAHYEVGRPDSYDKNDVFYLTSVQFAYVAGVNGIMTRERVATNFF
                     MGYFAAEALLMTETGNTIGAVQIAGSDAITQIPFFITTCDYTLIGEELYAASAYLNRE
                     PMLLGTLKAQDYFKFVILIFIIAGALLGTFQLTGLMQIFPVK"
     gene            3522..4148
                     /locus_tag="PK015_06720"
     CDS             3522..4148
                     /locus_tag="PK015_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34183.1"
                     /translation="MKKTIPLLIVIIGGVIWLAWVFVPHRLIQDVFYGEFYLPWARAM
                     APFAMLLGVISLSMMHYGKIKRKAPKWQYSFFFFAGFIVTSLAGFIGGTQKGGLYMWI
                     FENMQMPMSATMFSLLAFYMASAAYKAFRARSPEATVLLIAAIVVMLAQVPFGVKISK
                     YIPDISQWILDVPNLASKRGILLGVGLGSVATALKIMLGIERSYLGGD"
     gene            4150..>4692
                     /locus_tag="PK015_06725"
     CDS             4150..>4692
                     /locus_tag="PK015_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34184.1"
                     /translation="MAEKKSFFEWMQNLDRRWIYLIVALAIIIPLLIPYNSDNVTTPP
                     TENVYQMIDSFAGREDRAVLLSFYHDSSVMPELYPMEIAIMRHCFERNIKVFCLSFLP
                     TGAPIIDMAINTVKEDYPNIKSGVDYCNFGYIPAALILPTVIGMGDNIATAVPTDAEG
                     RKTENLPIMKGITNYNEMNLV"
BASE COUNT         1445 a          888 c          876 g         1483 t
ORIGIN      
        1 aaggagcttg ttgttaataa tgtattaaaa actaagattt atattctttg tctttataac
       61 aactaaagaa ggaaaagcct tacagttttt acgatttctc cctttttaag tcttaattta
      121 tattgacaaa aatcctaaac tttcagcaaa tggattctat aagtgggtga ttagctcagt
      181 ttggttagag tgctacgttg acatcgtaga ggtcactggt tcaactccag tatcacccac
      241 cattaattta atagggtgat tagctcagtt ggttagagcg ctacgttcac atcgtagagg
      301 ccactggttc gagtccagta tcacccacca taatcgtgtc aattcattcc ttatctatca
      361 aaaagtaaaa tagcataaaa aagcatttga caataaagcc aattttttgg tattgaattc
      421 caactcgttt tttagatatc ctgtcgttta ggagtttgaa tatgaaattg aaatattatg
      481 ccagtagctt gttgttgctc tttttaattt tctttggctg tagcagtcaa aagaaagcag
      541 ataattccaa tactggtaat ttcccggtgg aaaatttgca gattggagat attccaaatg
      601 atggtggcga tggcttgttt ctaagctgga aacccttacc taaagaaaag cgagtgcagg
      661 aatatcgcgt ttatcgcgga atccatcccg acactctttt ctttcttgct tcagtccagg
      721 taaatgtgaa aacaggagtg gcagcagata ctatgttctt ttatgatagt ggcacaagtg
      781 atttcctttc tatcgaatct cccaaaaaat taaaatatga aaaaggtgcc aagggttcaa
      841 atctttatag aggtataccc agagaccctg aaattgctgc ccgcttaagc gaaagctttg
      901 accttataag tattatggat gatagtgatt attactatca ttccaaaaaa agctattctg
      961 ctgatgaaaa agataaggat aaggataagg atgtctatgc cggtgtacga ttagaccagc
     1021 aaagaattgt agctgcctta aaaagcacac ccttaggaca aactcctcag tataaatatt
     1081 attacaccgt tgttccagtg gatgaacgta atcaaattct cggtattgcc gaacctgtct
     1141 ctgcttctcc tatagatgat attcctcaaa cttctcctgc attatatagt gtagccgttc
     1201 aagataaaaa agaaatgcaa cttgaatggg actatcctat caatagtgat gacctgatga
     1261 tgtacttgat ttatgctgtt cctggaataa ctaaagattt gtggaaaact cttaccccac
     1321 aggaaaaaga gcaaattact tctaccagag gcattttagt ggcacaaggt ctagtgggtg
     1381 gtggtgcttt aaaaaataat tgtataatca aagaagcaga ttttaaggct gcaggtttaa
     1441 attgggaaca agcttatcaa accctttata ctttaaaatt tgtggatggc tctaataata
     1501 tttctcccgt aagtgaagct tctcttccca aagtcattaa tagttcccaa cttccttctg
     1561 ctcctaaata tagagtggaa gacaaaccta tggataaagg tgaccgtctc acccttactt
     1621 ggcaagaacc tattgtattt ctcactcgta ctacttccca taaaaaagat ggctctcgcc
     1681 tcaaagttaa ttatcagata aataagactg atgctcagga tatccagaac atttattttg
     1741 acttctatga accaggttcg gatattccct ttgctcaaat aaatgaattt caccaggata
     1801 atattatcta tgtagacatt ccccaaaaat attccttgcg aaacggaggc aaattaccta
     1861 cagacagcct taaagtagag ataaccatta atagtagacc ttatagcatt gatcccaaaa
     1921 ctggaagaat acttcatgat aaagcccgaa ttattcctga ctataaaatt attcaatacc
     1981 tgaaacctga tcctgcaatg ctggcttata tgcctacaaa ttcttttata gttaatggac
     2041 ataatgtatc caccatcaaa aatgtggtct atagaaaagg ttatcgtagc tctaatttta
     2101 ccaaaattaa atctaatacc tgctatgaaa attttctgga tgtctctgtc ggatatattt
     2161 cttctattac taagcctatc ctcgggttta attttgttaa agatggcaaa ttatatacct
     2221 atatagatgg aaaacgatat gtacggaatc tccagccagg agaaaaagct tccagtctgg
     2281 ctttacttcc ttctactata gatttcactt atgatccggt aaacaaaacc acacttaata
     2341 tcagcatcta cctggatgaa gcccagaaaa aacttactaa actttcagat gatataaaag
     2401 aatcccaaca aaaactcgct gcttataaag attctctgac agctgctact cctgctatgg
     2461 caattcttta ccaagaaaat ataaacagac tggaacaaga gattaatacg aaagaatcgc
     2521 aactgaagat ttatcaggaa aatccttact ttcaagaagc tctgaaagca agaaatagtc
     2581 atcaaatgat gcgctatgtc gcttctatcc gtgaaccaga attaagaaaa tatacctatt
     2641 ccatagtgcg aactaatgaa aaaggatttt ttgccgaaac tcctcctgat gttaataagg
     2701 aaggtgaatt taactattat acaccaatct caaattggtt tgattggacc aaactggtta
     2761 ctttgatagc ggtatttctc tttggtatag atgtggttat atttatcaat cttgccaaac
     2821 gaggtaaaaa tctttatctt cgtcctttag ctggtttgca agaaattgat aatgctgtcg
     2881 gaagagctac tgaaatggga agacctatcc tctattgtat gggaattggt ggtttatcgg
     2941 atgtggcaac tattgcttct atgggaatct taagtcaggt ggcaaaaaag gcagctgaat
     3001 atgacactcg cttaattgtc ccctgctatg actatctggt tatgcctatt gcccaagaaa
     3061 tagttcagga agctcactat gaagtaggtc gtcctgatag ttatgataaa aatgatgtgt
     3121 tctacctcac ttcggttcaa tttgcttatg tagcaggtgt taatggcatt atgactcgtg
     3181 aaagagtagc caccaacttc tttatgggat actttgccgc agaagctttg ctaatgacgg
     3241 aaaccggcaa tactataggt gctgtccaga ttgcaggttc tgatgccatc acgcagatac
     3301 ctttcttcat caccacctgt gactatactc ttattggtga ggaactctat gcggcatctg
     3361 cctatctcaa tcgtgagcca atgcttcttg gcactttaaa agctcaagat tatttcaagt
     3421 ttgtcatttt aatatttatt atcgctggtg ctcttctggg cactttccag cttactggtt
     3481 taatgcaaat tttccctgtt aaataaaaag aggtcttatc tatgaagaag acaataccat
     3541 tattaatcgt cattatcggt ggagtaatct ggcttgcctg ggttttcgtt ccccaccgtc
     3601 tcatccagga tgttttctat ggagaatttt atttaccttg ggctcgtgcc atggcacctt
     3661 ttgctatgct attaggtgtt atcagcttgt ctatgatgca ctatggcaaa attaaacgca
     3721 aggcacctaa atggcaatac agctttttct tctttgcagg attcatagtt acatccctgg
     3781 caggttttat tggaggcaca caaaaaggtg gcttgtatat gtggatattt gaaaatatgc
     3841 aaatgcctat gagtgcaacg atgttctctc ttttagcatt ctatatggca tcagcagcct
     3901 acaaagcatt ccgagcacga tctcctgaag ccacagtact tttaattgca gccatagtcg
     3961 taatgcttgc tcaggtacct tttggtgtta aaattagcaa atatatcccc gatatttctc
     4021 agtggatact ggatgtgcca aatcttgctt ctaaaagagg tatcctcctg ggggtagggt
     4081 taggaagtgt tgctactgct ctaaagataa tgctgggaat tgaacgctcc tatttgggag
     4141 gtgattgaaa tggctgagaa aaaaagtttc tttgaatgga tgcaaaactt agaccgtaga
     4201 tggatttatc tcatagttgc tctcgctatc attatccctt tgcttattcc ttataattct
     4261 gataatgtga ccacacctcc aacagaaaat gtctatcaaa tgatagattc ttttgccggt
     4321 agagaagata gagctgttct attaagcttc tatcatgatt cctcggttat gccagaactt
     4381 tatcctatgg aaatagcaat aatgcgacat tgctttgaac ggaatattaa ggtgttttgc
     4441 ctttctttcc tacctactgg tgctcctatc attgatatgg caatcaatac ggtcaaagaa
     4501 gattatccta atattaaatc gggagttgat tattgcaact ttggttatat tcctgccgcc
     4561 ttaattctcc ctaccgtgat aggaatggga gataatatcg caactgcagt tccaaccgat
     4621 gcggaaggaa gaaaaactga aaatcttccc ataatgaagg gcataaccaa ttataatgag
     4681 atgaatttag tc
//
LOCUS       DAORZF010000066         4376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_13450_length_4376_cov_344.559130, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000066 DAORZF010000000
VERSION     DAORZF010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13450_length_4376_cov_344.559130"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..590
                     /locus_tag="PK015_06730"
     CDS             114..590
                     /locus_tag="PK015_06730"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOL34185.1"
                     /translation="MKQTDLSTAYGLIHLPAKVALAVTEKPQGGYNLITIEWFMRTSI
                     QPPMFAISIGHTRFSHECLQQNRFFNLVFPSVEMKDLCALAGSTTGREIDKMEYGKVR
                     YFPGKLHRIPVLEDAVAVFECEIVTQVRSGDHTIFVGEIKYCWADKEKKLLVYTME"
     gene            1013..1207
                     /locus_tag="PK015_06735"
     CDS             1013..1207
                     /locus_tag="PK015_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34186.1"
                     /translation="MSKLSTNSSVSQSNQIHHKSMSQLTEIEQENIPVPISSSPIYEN
                     NNLSIVQTIQKNQLIKEVKK"
     gene            1204..4053
                     /locus_tag="PK015_06740"
     CDS             1204..4053
                     /locus_tag="PK015_06740"
                     /inference="COORDINATES: protein motif:HMM:NF024547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HOL34187.1"
                     /translation="MKDSIQSIFPEIQGSQCYTVSEDTISEPNFNDVITLQTEDNLLS
                     AEEAIQTSNNTNNIKGTNIMTKSITNSKESINYTGVKMKQSFQSIVPEIQGSQCYTVS
                     EDTISEPNFNDVITLQTEDNLLSAEEAIQTSNNTNNIKGNNIMTNTTINSLPVNQETQ
                     LSLSDESIATTQYNNHPEISLNPLLSEDTMMSLEKAVELNNNIMELKMTKLKDNVRNS
                     SVSHRNSANENDPLTLVTMGKDVKNPELLEYRTLDEIINDIKSGQHKAKIETIRNTAN
                     VEERKALKTKLPYFIYGIIQDRRSDGNVRQMNGLIIDIDDVADIEEGKKELEALPCAR
                     YIFRSPYNGIKVIIPFNRPVTDKDTYMTLWKYCALNIRDLIGTEPDDPSGWSQACFIS
                     YDPDIGDLGEEHFLNVEDTILIMLQTEEKSESSHEQKCNQYSQEETINNSISAVEHLS
                     QRKIKYRDWLRCGIAIYNYYKDIGKLDEGKTLWLSFADNPNYQDTDRELEKIWEKIAK
                     NTYSQIHIGTLFYIAQEYGWKVPKSQQNNIVLMVPLDKSKLPPMFQEYIETVNKITNA
                     PDGVILTAMLPYLAVSIGNHMYIKAFGIKHYCNIYAILIGPSSRTHKSTCMNLARYIL
                     KEENYDLIRNKITDPALLKELKNKPNLLLVFSEMGSLFQNFKQDYNKPMVDDITDLFN
                     GYFNGNSTLDYSVFIKDTALSIIGAITNDSFDDASKHIIDRGCGFFQRFISCYVPVNY
                     YSYNDVIYKRTQVSFDDIRKYNDMTSFFVSLPGSYELRYNAEAESYINGEYSIKYNQL
                     QQKEGREGEFNTRLYTDYLYRFSIMIYAVKRWEDMREAKDLEEWFEENPIDKETVLQA
                     EYMCDYYRKNSILIISSWDDKYTLDNMQKLIKCLDSSPEHRLNKTNISKAFNNHLSKE
                     KMEKYIHYLLELDLIEQEEEIVRNSNNPPTYFKLK"
BASE COUNT         1554 a          757 c          718 g         1347 t
ORIGIN      
        1 attaccatag aaagagtaag taagattata ccgtgattat ttacctcttt cttataatag
       61 atttttatct tgagcagatt ttattatatt atctaaaaaa gataggataa agtatgaaac
      121 agacagacct ttctactgct tatggtttaa ttcatttacc agctaaagtt gctctggcag
      181 tgacggaaaa acctcagggt ggttataatc taatcaccat agaatggttt atgcggactt
      241 ccattcagcc tcctatgttt gctatttcca tcggtcatac cagattttct cacgaatgct
      301 tgcaacaaaa tagatttttt aacctcgtct ttccatcagt ggagatgaaa gacctctgtg
      361 ctctggcagg ttctaccacg ggaagagaga ttgataagat ggaatatggc aaagtaagat
      421 atttccctgg taaactacat agaatacctg ttttagaaga tgcagttgcc gtctttgaat
      481 gcgaaatagt cactcaggtt cgtagcggag accataccat attcgtaggt gagataaaat
      541 actgctgggc agataaagag aaaaaactgc tggtgtatac tatggagtaa aaataaatca
      601 agaaggacac agtaaagatt tgctaacatc atattgaaga ataagttaaa acatacatca
      661 tttttttctc ttgacaaaga tagtatgttg gttataatag tatcattatg attaaaatat
      721 taattttaaa tctaaaagaa atccttacgg gatgtaagga aggaggaagg tatgccctta
      781 aacgacaata catttaataa ctcatttgtt tctcacagaa ataaccactc acccaattca
      841 atcaattcat tgagaatggc gaaatgcaaa tgcaaatatc tatctatgct taacttacat
      901 taccaccata ttttcttagt attagctgaa aaaaatccct atattaaagc attattatta
      961 tcctatgagg cagaattatt atttgcagaa tacaaacaac gaggagcata ttatgagtaa
     1021 gctaagtaca aactcatctg tctcccaaag taatcaaatc caccacaaat caatgtcgca
     1081 attaactgag attgagcagg aaaatattcc tgtcccaatt tcgtcatccc ctatctatga
     1141 gaataataat ttatccatag tacaaaccat tcaaaagaat caattaatta aagaggtaaa
     1201 aaaatgaaag attcaattca atcaatcttc cctgaaattc agggttccca atgttacaca
     1261 gtttccgaag acactatctc ggaacctaat tttaatgatg taatcacgct tcaaacagaa
     1321 gataatcttc tttctgccga agaagcaatt caaacctcta acaatacaaa taatattaaa
     1381 ggaacaaaca ttatgaccaa atcaattacc aattcaaaag aatcaattaa ttacacagga
     1441 gtaaagatga aacaatcatt tcaatcaatc gtccctgaaa ttcagggttc ccaatgttac
     1501 acagtttccg aagacactat ctcggaacct aattttaatg atgtaatcac gcttcaaaca
     1561 gaagataatc ttctttctgc cgaagaagca attcaaacct ctaacaatac aaataatatt
     1621 aaaggaaata atatcatgac aaacacaact atcaattctc ttcctgttaa tcaggaaacc
     1681 cagctttctc ttagtgatga aagcattgct accactcagt ataataatca tcctgaaatt
     1741 tctttaaacc ctcttctctc agaggatact atgatgtcct tagagaaggc tgtagaactt
     1801 aacaataata ttatggagtt aaaaatgact aagttaaaag acaatgtgag aaactcatct
     1861 gtttctcata gaaattcagc aaatgaaaat gaccccttaa ccctggttac tatgggtaag
     1921 gatgttaaaa acccagagtt attagaatat agaactctgg atgaaataat taatgacatt
     1981 aaaagtggtc agcataaagc taagattgaa actatcagaa atactgccaa tgtagaggag
     2041 cgtaaagctc tcaagacaaa actaccctat ttcatctatg gtataatcca agaccgtaga
     2101 tctgatggta atgtccgtca gatgaacggt ctgattatag atattgatga tgttgcggat
     2161 attgaggagg gtaagaaaga gcttgaggct cttccgtgtg ctcgttatat ctttcgttct
     2221 ccttataatg ggataaaggt tattattcct tttaaccgtc ctgttactga caaagatact
     2281 tatatgacat tatggaaata ttgtgcatta aatattcgtg atctaatcgg aacagagcca
     2341 gatgaccctt cggggtggtc tcaagcctgt ttcattagtt atgatccaga tataggggat
     2401 ttaggagaag aacattttct caatgtagaa gatactatct taattatgct gcaaacggaa
     2461 gagaagagcg aatcttctca tgagcaaaaa tgcaatcagt attctcaaga ggagaccatc
     2521 aataattcta tttctgcagt agaacattta tcacagagga aaattaaata tagggactgg
     2581 cttcgttgcg gtattgctat ctataactat tataaagaca taggaaagct ggatgagggt
     2641 aaaaccctct ggctttcctt tgccgataat cccaattatc aggatacaga tagggaatta
     2701 gaaaaaatct gggaaaagat agccaaaaat acttatagcc aaatccatat aggtactctc
     2761 ttctatatag cccaagaata tggatggaaa gttccgaaaa gccagcaaaa taatattgtc
     2821 ttgatggtac ctctggataa atctaagtta ccacctatgt ttcaggaata tattgagact
     2881 gtaaataaaa ttacaaatgc tcctgatggg gttattctta ctgcaatgtt accctatctc
     2941 gcagtgtcta ttggtaatca tatgtatatt aaagcattcg gaataaaaca ctattgtaac
     3001 atttatgcca tattaatagg tccttcttca agaacccata aaagcacctg tatgaacctg
     3061 gctagatata tactaaaaga agaaaattat gatttgatta gaaacaaaat taccgatcca
     3121 gctttgctga aagaactaaa aaacaaacct aatctgctat tagtattttc agaaatgggt
     3181 agtttgttcc agaacttcaa acaggattat aataagccta tggtggacga tataaccgac
     3241 ctgtttaatg gatattttaa tggtaatagc actctggatt atagtgtctt tataaaagat
     3301 actgctctat ctattattgg cgctataacc aatgatagct ttgatgatgc ttctaaacat
     3361 atcattgata gaggttgcgg tttcttccag agattcatat catgttatgt accagttaat
     3421 tactattctt acaatgatgt catatataag cgtacccaag tttcatttga tgacatacgt
     3481 aagtataatg atatgacctc cttttttgta tctctaccag gttcttatga actgaggtat
     3541 aatgctgaag cagaatcata tatcaatgga gaatatagca tcaaatataa tcagctgcag
     3601 caaaaagagg ggagagaagg ggaatttaat actcgtctct atactgatta cctttataga
     3661 ttctctatta tgatttacgc cgtgaaaagg tgggaagata tgagagaggc taaagacctg
     3721 gaagagtggt ttgaagaaaa tcctattgat aaagagactg ttcttcaagc tgaatatatg
     3781 tgcgattatt atcgtaaaaa tagtatatta attatatcgt cctgggatga taaatatact
     3841 cttgataata tgcaaaaatt aatcaaatgc cttgactctt ctccagaaca tagactcaat
     3901 aaaaccaata tctctaaggc atttaataat cacctatcta aagaaaaaat ggaaaagtat
     3961 atccattact tactggagtt agaccttata gaacaagaag aagaaatagt tagaaattca
     4021 aataaccccc ccacctactt taagttaaag tgatagataa acataagggg ggagtcttcg
     4081 gactccccct ttttcattcc ttaatatttt cttccagttt agataagatt taaggttttg
     4141 ttaattttag tcttaggact tccttatttc cttgcttaat aaattcccac tttaccgaag
     4201 tggtatgcct acaataatca cttataattc tcctgcttct ttaataaatt cgcataaaat
     4261 ttaataaatt cgtagcctgt tttggacaca taatgaactt atctacttca atcatataat
     4321 gttataacat ttatgcctca cccttatacc ccccttatct atcacttatc atttta
//
LOCUS       DAORZF010000067         4126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_14489_length_4126_cov_283.643822, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000067 DAORZF010000000
VERSION     DAORZF010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14489_length_4126_cov_283.643822"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            248..463
                     /locus_tag="PK015_06745"
     CDS             248..463
                     /locus_tag="PK015_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34188.1"
                     /translation="MKSGSQLILKPDEGMEQPVEVLTKQSGTLKLWTTMKVIEMTKSG
                     FDKEPNKPDPYAGEYHYKSWWNQFREP"
     gene            1260..3749
                     /locus_tag="PK015_06750"
     CDS             1260..3749
                     /locus_tag="PK015_06750"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOL34189.1"
                     /translation="MKKTCFLLAFILAICFLFAQLSDPQSDILDLISLVNADTLRAYV
                     QHLQDYQTRYALADNHLEVANWVKGQFESYGFTNTWMQEYQHNNTTQYNVIATIPGYL
                     YPDLYIIVGGHYDSYSANSDSYVFAPGADDNASGTAGVLEIARVMMAIGYQPKFSIRF
                     IAFSAEEGNGWGSEAYCNYAINANQEIRVMLNLDMIANNLQTSQEFRVMPYEGSEEYS
                     YETIRIAQQYSSYQPVLGETGMGSDSYIFSENGFNAVFFFERYLNPYLHSSDDLIDYL
                     DFQYASEIVRAATAATVIFANQPFPVNNIQVYNPGTGNSLLAIWDSSSDPEVTKYAVY
                     YGTEIDSMTFWQYINENQCIISGLEEGQSYYIAVCNVNDDGLTSIRKYGSGTPFSVPA
                     APNLIADFPDRHSITITWQPNSELDIASYSIYRSLGSNTTETMIATVPVPDTSYTDTD
                     VKSNEEYYYYRLTANDSEGNQSPFSSPLASRMVSLDRGIYVIDESKNFSGTTPFQPTD
                     EAVDNFYNDLLKNYEKVSLLDLEEQTGILKLADIGIYSSILWHGNDIGDNTYPYEIRD
                     ILGEYIRLGGNIFFTVYYPSKAFELNAGYPASFSEDSFINEVLGIGGVSYSVQSRFKY
                     AIPNNENYPALQVDSLKTISSWHSHIFGVEGMEPVNPEESIYLYGSDYSPESNQGSLN
                     GKSVGIHRYYGEGQVLCLSFPLYNMQASSAQDLIDYVFTNLFQESTKPDIPIPPVTGL
                     RLLPNYPNPFVNETTFTVINADPEKLMNIKIYNLKGQVVQTIFNGYPLQDMQFTWNGK
                     DTTERNTSSGIYFVKVSSGKEKVIAKIIKIK"
BASE COUNT         1318 a          718 c          829 g         1261 t
ORIGIN      
        1 atatcacgat tttttcaatc gtatttcgta ttgcattgta tgacttgcga ttatgatagg
       61 tgctcattca atctgattgt ttattgagac atatatcttt cttatcatat tgagcggaaa
      121 gtatttgacc acctttgcga ggggtggaga gtagcaccca taaattaaaa gttgggtaac
      181 cgagaaagag atcaagatga ctaaccaaaa caagaatcgc taaagccaag taatgctgga
      241 ggagatagtg aaatctggca gccaacttat tttgaagcct gacgagggga tggagcagcc
      301 agtagaagta ttaaccaagc aatcaggcac attgaagtta tggacaacaa tgaaggtaat
      361 cgagatgacc aagagtggct ttgataaaga gcctaacaag cccgatccct atgctggtga
      421 ataccactac aaaagctggt ggaatcagtt ccgagagcct tgattgcagt cagagaagat
      481 gaccgaagat gagatcaaaa ccagagtcga tgatgcctgg gaatattgag actacaagga
      541 gatgatcaga ttgttggaag agttgatctg catgatcgaa aagaagcgat aatcaacatc
      601 tatccagttc attaacccgt ttctgctgag ttttatcttt ggctgggttc tatttggcga
      661 gggcaagaag cttgtagaaa ggattagttg tggtatagac ctgactgatc ctgatcataa
      721 tagtgactct gttcacattg agtttaacaa cggttatata gaggaaaaca tcgcactcgt
      781 tggtcaggat gatgtgaagc agctcctgat actctttatc cagaagaaag atgaaagata
      841 ttcaaacttg acttggagac acccaaatcc gattatggtc atagcctaca gaccctacat
      901 agaatgccct ctgatatgat atgtggtatg ctgacagtgt ttcccaagtt ttattattga
      961 tatgcagaac ccatttgaag ttttcagtga tataagtttg aaattttcgg tgatacgatt
     1021 tgaagttttc actgatacat tacaatagat ttagttaggg aaagtatgtt taactatatt
     1081 ttaattgaca aataagtttt agaatatcta aataactcta tgaagaaaaa tagttacggc
     1141 aaatggataa actggctaaa aaaccatatt attccggtgt atgacacttt cctaacgaga
     1201 agaataaatc aaataaaaag aggacttttt tcgggtaaat atactgtata ggagaaacaa
     1261 tgaaaaagac ttgcttttta ctggctttca ttttagcaat ctgtttcctg ttcgctcagt
     1321 tgtcggaccc gcagtcagat attttagatt taatttcctt ggtgaatgcc gatactttaa
     1381 gagcttatgt tcaacacctg caggattatc aaacccgtta tgctctggca gataatcatt
     1441 tagaggttgc aaattgggta aaaggtcaat ttgaaagtta tggttttacc aatacttgga
     1501 tgcaggaata tcagcataat aatactactc aatataatgt aattgccact attcctggat
     1561 atttatatcc ggatctttat atcatcgtag gaggacatta tgattcatat tctgccaatt
     1621 ccgattctta tgtctttgct cccggtgcag atgataatgc cagtggcacg gcaggtgtat
     1681 tagaaattgc cagggtgatg atggcgatag gatatcagcc aaaattttcc attcgtttta
     1741 tcgcttttag tgctgaagaa ggtaatggtt ggggttctga agcgtattgc aattacgcca
     1801 tcaatgcgaa tcaggaaata agagtgatgc tcaatctgga tatgatagcg aataatttgc
     1861 agactagcca agagtttcgg gttatgcctt atgaaggttc tgaagaatat agttatgaaa
     1921 caataagaat cgcccaacaa tatagttctt atcaaccggt tttgggagag acgggtatgg
     1981 gttccgatag ctatatcttt tcagaaaacg gctttaatgc cgtatttttc tttgaacgct
     2041 atctcaatcc ctatctccat agcagtgatg atttgattga ttatctggat tttcagtatg
     2101 ccagtgaaat agttcgggca gctactgctg ctactgttat atttgctaat cagccctttc
     2161 cggtgaataa tattcaagtt tataatcccg ggacaggaaa ctctttactg gcgatatggg
     2221 atagttcttc cgatccggaa gtaactaaat atgcagtgta ttatggaaca gaaatcgatt
     2281 caatgacttt ttggcaatat ataaatgaaa accaatgtat tattagtgga cttgaggaag
     2341 ggcaatctta ttacatagct gtttgtaatg taaatgatga cggcttaaca agtatccgta
     2401 aatatggaag cggcacacct ttttcggttc ctgcagcgcc aaatttaatt gccgattttc
     2461 cagatagaca ttctataact attacttggc aaccaaattc ggaattggat atagcaagct
     2521 atagtatcta tcgttctctg ggttcaaaca caacggaaac aatgattgct actgttcctg
     2581 tccctgatac ttcttatacc gatacggatg taaaaagtaa tgaggaatat tattactatc
     2641 gccttactgc caatgattcg gagggaaatc aaagtccctt ctcttcacct ttggcaagta
     2701 gaatggtttc cttggataga ggtatttatg tgatagacga gagcaagaat ttcagcggaa
     2761 caactccttt tcaaccgact gatgaggcag tagataattt ttacaatgat cttttaaaga
     2821 attatgaaaa ggtctcccta ttggacctgg aagaacaaac cggaatcctg aaattggcag
     2881 atatcggtat ctattcatct attttgtggc atggcaatga tattggcgat aatacctatc
     2941 cttacgaaat tcgtgatatt ctaggggaat atattcggtt gggaggaaat atatttttca
     3001 ctgtctatta tccgagtaag gcatttgaat tgaatgcggg atatccggca tccttttctg
     3061 aagacagttt tatcaatgag gttttgggca tcggaggggt aagttacagt gtgcagagcc
     3121 gttttaaata tgctattcct aacaatgaaa attatcctgc tctccaagtt gacagtttaa
     3181 aaaccatatc ctcctggcat agtcatatct ttggcgtgga gggaatggaa ccggtaaatc
     3241 cggaagagag tatttatctg tatggttccg attatagccc agaaagcaac cagggctcct
     3301 taaacggaaa aagtgtaggt attcatcgct actatggaga aggtcaggta ttatgtttaa
     3361 gtttccctct ctacaatatg caggcgtcat ctgcccaaga tttgattgat tatgtcttta
     3421 ctaacctttt tcaggaatcg accaaacctg atattccaat accaccagta acaggactta
     3481 gattattgcc aaattatcct aatccctttg taaatgaaac aactttcact gtgataaatg
     3541 ccgatccaga aaagttaatg aatattaaaa tctacaacct taaaggacag gtagttcaaa
     3601 ctatttttaa tggatatcct cttcaggata tgcaatttac ttggaatggc aaagatacta
     3661 cagaaagaaa cacaagctct ggaatttact tcgtgaaagt atcctcaggt aaagagaaag
     3721 taattgctaa aataatcaaa ataaaataat taccctcacc aacaaccacc aattacattt
     3781 cttaaacttt tccctaaagt ttctttagtc ccgttaagga agtgttagag gatctcctaa
     3841 gagatctatg cgttctcact gtggtgtcct aaataagttg acacaaaata cattaaagga
     3901 gttatagaaa tgaagacaaa atttgataga atcgtgatac ttccaaaaaa gtccaaaaac
     3961 cactgtgata cagtctaaag ccagtcctat ctaactctgc accctataaa cctttaagac
     4021 ctctgtgaca catgatatca atttgaaaat ttgggtgaga acttataatt aactctattc
     4081 tttatattcc actgcaaatt atgtaattac tccctctaaa taaata
//
LOCUS       DAORZF010000068         4118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_14526_length_4118_cov_370.009353, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000068 DAORZF010000000
VERSION     DAORZF010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14526_length_4118_cov_370.009353"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            515..2641
                     /locus_tag="PK015_06755"
     CDS             515..2641
                     /locus_tag="PK015_06755"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HOL34190.1"
                     /translation="MFKKIRKRNGSIVDFDQQKIVRAIQKAGEATGEFQEDTAELLAN
                     RVLNLAAQIINTEIPEVERIQDIVEEVLLASPYKKTAKAYIIYRDQHARLREMFNTGG
                     VQLIDKYLEKLDWQVNENSNMAYSLQGLNNYIASEISKTYWLNKIYPPEIRQAHLEGD
                     IHIHDLGLLSVYCVGWDLMDLLTTGFSGAEGKVESSPAKHFRSALGQIVNFFYTLQGE
                     AAGAQAFSNFDTLLAPFIRYDHLDYKEVKQALQEFIFNINVPTRVGFQTPFTNITLDL
                     KPSSLFKDMPVIIGGKPQAETYGEFQKEMDMLNQAFLEVMIEGDAKGRVFTFPIPTYN
                     ITKDFDWDAPQLNYLWEATAKYGIPYFSNFINSDMDPNDARSMCCRLRLDTRKLASRG
                     GGLFGANPLTGSIGVVTINLPRIGYKAKNEEEFFEYLEPVLVIAKNSLEIKRKVLERY
                     TENGLYPYTRFYLRNIYERFGEYWKNHFSTIGIIGMNEACLNLFGKSIATPEGREFAL
                     KVMDYLRQRLLDFQEETECNYNLEATPAEGASYRLALLDKKHFPDIITANCDDEAPFY
                     TNSTHLPVNYSDDIFEVLDLQDELQTKYTGGTVLHIYAGERVEQGESIKKLVKTVCEN
                     YRLPYFTFSPTFSICPQHGYLIGEQPICPQCHQSTEVFSRIVGYLRPVSQWNEGKKAE
                     FKLRKTFHPIKHIETKEQHQEKTHLP"
     gene            2653..3372
                     /locus_tag="PK015_06760"
     CDS             2653..3372
                     /locus_tag="PK015_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HOL34191.1"
                     /translation="MKIGGFQKFSLLDYPGELAAIIFTQGCNFRCPYCHNPELVDVAK
                     ADSLYPEKQILDFLKSRKGKLTAVVIGGGEPTLQEDLEDFLDKIKAMNYLIKVDTNGS
                     IPESIENLVNKDLVDYWAMDLKAPESLYPLVTRADIPFSSILKSMEIIRESSKSYEFR
                     TTFFDKLLHLEDLEKIQNLLRPGDKFYLQECQYSKTLDNLSSSAEIDSPSFLNWQNQP
                     FLQSLIQWGNKNQVKIEIRKL"
     gene            3376..>4118
                     /locus_tag="PK015_06765"
     CDS             3376..>4118
                     /locus_tag="PK015_06765"
                     /inference="COORDINATES: protein motif:HMM:NF040068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34192.1"
                     /translation="MDKIQIEPWLLQVNKPSRYIDQEINAIRKPWQKVNFCFVYPDVY
                     EVGISNLGLKILYSIVNRIEEVMADRCYLPWIDMIEIMHREKIPLFGLESRNALKEFD
                     LIGITLQSEITYSNVLETIDLAGIPVFAKDRGEEYPIIMAGGPCAINPLPLQDFIDVF
                     FIGEAEEGIMEIAEIFKNYKGKEKRLQKLAEIESCYVPKIHSPLISQGWKVKARKFTA
                     FSHSVLIHKPQLLPWQIATHHRCVAELMRG"
BASE COUNT         1334 a          720 c          787 g         1277 t
ORIGIN      
        1 aaggaaaata gggcagaccc tttatctact aaagcaaata caatataaat accacttttc
       61 atcctgaatt ctatatatct attataatta atctccttta tctactcgca ttatttccag
      121 ctaatctgga ttaattttgc ttccctgttc acaattctcc cttagctctc tcttagctct
      181 tacttagcta attccttaag agagaagcaa gtaggagtta ggtgacaagc aagaatggct
      241 aaaccattaa atgagtgata gttaaggaga acccataaaa atatttttct tactcttaag
      301 ctaataaaat atggggtcta ttcctttata attattgatt taaattaaat tcaggagggt
      361 gaaattagaa aaatggataa gtttatttat cttgacaaaa tcataatcct aagaataaga
      421 aataatagcg agtgtgataa tagatctttg gaaaatgcct tattaagata taatatattg
      481 ataaataata aataaatgat tttgggagta gtgtatgttc aaaaagatta gaaaaaggaa
      541 tggttcaatt gttgattttg accagcaaaa gattgtgcga gcaattcaaa aagctggtga
      601 agctacagga gaattccagg aagataccgc agagcttctt gctaatcgtg tgctcaattt
      661 agctgcacaa atcataaata ctgaaattcc tgaagtggaa cgcattcagg atatagtaga
      721 agaggttttg ctcgcttctc cctataaaaa gacggcaaaa gcatatatca tttatcgtga
      781 ccaacatgct cgtctccgag aaatgtttaa tactggtgga gtgcaattaa ttgataagta
      841 tctggagaaa cttgattggc aggtgaatga gaattccaat atggcatatt ctttgcaggg
      901 tttgaataat tatatcgcca gtgaaataag taaaacctat tggttgaata agatttatcc
      961 gccagaaatt cgtcaggcac atctggaagg tgatattcat attcacgacc tcggtctact
     1021 ctcggtttat tgtgtaggat gggatttaat ggatttattg accactggct tttctggagc
     1081 tgaaggaaaa gtggaaagtt cgcctgcgaa acattttcgt agtgctctcg gtcagattgt
     1141 aaactttttc tatactcttc aaggtgaagc tgctggagcc caagcatttt ccaattttga
     1201 taccttatta gcacctttta ttcgttatga ccatctggat tataaggaag tgaaacaggc
     1261 tttgcaggaa tttatcttca acatcaatgt tcctaccagg gttggatttc aaaccccatt
     1321 taccaatatt acgcttgatt taaagccatc ttctctgttt aaagatatgc ctgtaatcat
     1381 cggaggaaaa cctcaagctg agacttatgg tgaattccaa aaagagatgg atatgcttaa
     1441 tcaagctttt ctggaagtaa tgattgaagg agatgccaaa ggaagagttt tcacttttcc
     1501 tattccaacc tacaatatca ccaaagattt tgattgggat gcccctcaat tgaactatct
     1561 ctgggaagcc accgccaaat atggtattcc ttatttttct aattttatca attccgatat
     1621 ggaccctaac gatgctcgtt caatgtgctg tcgtttaaga ttggatacca gaaaattggc
     1681 ttctcgtgga ggaggacttt ttggtgcgaa tcccttaaca ggttctatag gagtagtgac
     1741 catcaacttg ccgagaatag gttataaagc taaaaatgaa gaagagtttt ttgaatatct
     1801 tgaaccggtt ttggttattg ctaaaaacag tttggaaatc aagcgaaaag tgcttgaacg
     1861 ctacacagaa aacggacttt atccctacac ccgtttttat ttgaggaata tctatgaacg
     1921 ctttggtgaa tactggaaga atcatttttc taccatcgga atcataggta tgaatgaagc
     1981 ctgtctaaat ctttttggca aaagcattgc tacccctgaa ggaagagagt tcgctctcaa
     2041 agtgatggat tatctacgcc agagattgct tgattttcaa gaagagacag aatgcaacta
     2101 caatctggaa gcaactcctg cagaaggggc aagctatcgt ttagctctgc tggataaaaa
     2161 gcattttcct gatattatca ccgctaattg cgatgatgaa gctccttttt ataccaatag
     2221 cacgcatctg ccagtgaatt attcggatga tatctttgaa gtgctggacc tgcaagatga
     2281 actgcaaacg aaatataccg gtggaaccgt cctccatatc tatgctggtg aaagagttga
     2341 acaaggagaa agcattaaga aactggtgaa aacagtttgt gaaaactacc gtttacctta
     2401 ttttaccttt tctcctactt tcagtatttg tcctcagcat ggctatttaa taggtgaaca
     2461 acctatctgt cctcaatgcc accaatcgac tgaagttttc tctcggatag ttggttattt
     2521 gcgtcctgtt tcacaatgga atgaagggaa aaaagcagaa ttcaaattgc gtaaaacctt
     2581 tcatccaatc aaacatatag agacaaaaga gcagcatcag gagaaaactc atcttcctta
     2641 aggatttgtt ttatgaaaat tggaggtttc caaaaatttt ctttgctgga ttatccagga
     2701 gaactggcag ctattatctt cactcaaggt tgcaatttcc gctgtcctta ttgccataat
     2761 cctgaattgg tggatgtagc aaaagctgat tccctatatc cagaaaaaca gattcttgat
     2821 tttttaaaaa gtagaaaagg aaaattaact gctgtggtta tcggaggtgg tgaacctaca
     2881 cttcaagaag atttagagga tttcttagat aaaataaagg ctatgaatta tctcatcaaa
     2941 gtagatacca atggttctat ccctgaaagc atagaaaatc ttgtaaacaa agacttagtt
     3001 gattactggg caatggacct taaagcacca gaatcactct atcctttagt cacacgagct
     3061 gatattcctt tttccagtat cttgaaaagt atggaaatta ttagagaaag cagcaaaagt
     3121 tatgaatttc gcactacctt ttttgataaa cttttgcatt tagaagattt agagaaaatc
     3181 cagaatcttc ttcgtccggg cgataaattc tatctacaag aatgccagta tagcaagact
     3241 ttagataatc tttcttcttc cgctgaaata gatagtcctt cctttcttaa ttggcaaaat
     3301 cagccctttc ttcagtctct aattcaatgg ggaaataaaa atcaggttaa aatagagatt
     3361 cgcaagctat aatatatgga taaaattcag attgaaccct ggttactgca ggtaaacaaa
     3421 ccttcccgct acatagacca ggaaattaat gctattagaa aaccctggca gaaggtcaat
     3481 ttttgctttg tttatcctga tgtctatgaa gtgggcattt ccaatctcgg gctaaaaata
     3541 ctctattcta tagttaatcg cattgaagag gtaatggctg atagatgcta tcttccctgg
     3601 attgatatga tagagattat gcatcgtgaa aaaataccac tatttggatt agaaagcaga
     3661 aatgctttaa aggaatttga ccttattggc attactttac agagcgagat tacctatagc
     3721 aatgttctgg agaccataga tttagctgga attcctgttt ttgctaaaga taggggagaa
     3781 gaatatccaa ttattatggc aggaggtcct tgtgctatca atcctttgcc cttacaggat
     3841 tttatagatg tattctttat aggagaagcg gaagagggta ttatggaaat agcagaaatt
     3901 tttaaaaact ataaagggaa agaaaaaaga cttcaaaaac ttgcagaaat tgaaagctgc
     3961 tatgttccaa aaattcattc tcctctcatc tctcagggtt ggaaggtcaa agcaagaaaa
     4021 tttaccgcgt tttcccattc tgttttaatt cacaaacctc aacttctacc ctggcaaata
     4081 gctactcatc atcgctgtgt tgcagaactt atgcgagg
//
LOCUS       DAORZF010000069         3841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_15875_length_3841_cov_350.409667, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000069 DAORZF010000000
VERSION     DAORZF010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15875_length_3841_cov_350.409667"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(274..2172)
                     /gene="alr"
                     /locus_tag="PK015_06770"
     CDS             complement(274..2172)
                     /gene="alr"
                     /locus_tag="PK015_06770"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOL34193.1"
                     /translation="MDKKGKSLERSWVEIDLDAFRSNLKALKTFLKSEQGFIQVVKAD
                     AYGHGAWEITQAALKEGATALGVANLEEGKLLRIEGCKAPILILSPSLIDEIPGILEY
                     KLIPTVNELSFAQALVSFCAKQKVKCPISLKFDSGMHRSGALEKDFAELWSYCQNAAE
                     LEVKSVFSHFASSESDPEFSKMQEERFKQVLKKYKVQAEWIHIANSSALINSLGSSGN
                     LCRLGILSYGVYTHPSQKEKIELKPVMTFKSTIIQIKEIPKGATVGYNQTWKAKRKTR
                     YAVIPVGYADGYDFLLSNKGKVLIAGKLCPVIGKVSMDMICVDITDAPEIQYGTEVIL
                     LGNGHNDIRVENLVSLYNGSSYELLCQVGRRAKRYYYEGGRLVTAAPLSRRDFVSSDY
                     PNSKLNQIIQSAIAQRINSEEMSELIFREILRVFFYNQDRDIRYRKDFRHHILFTESS
                     DKDYWKAETTLSFSKTLQRDFFLVACANSDKALKEYFKRNEVEYRWLMDGNFQLNPSA
                     FQLSSVKVNDIELETRINFKSEAMEIRCSHPALKNLIGQEVRYEINTLTLYPKSSHQL
                     SVFITELTHGVQISFSYPETLKQIECVPFFAGQNKYPKITTSKNIITVTTKPEEWVFP
                     QSGVVFAY"
     gene            complement(2182..3522)
                     /locus_tag="PK015_06775"
     CDS             complement(2182..3522)
                     /locus_tag="PK015_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOL34194.1"
                     /translation="MAQKIFVLDTNVLLHNPQAIFSFQDNRVVIPIIVIEEIDQFKKG
                     VDEKSRNARQIGRYLDALRSKGSLQEGVKLDNGGILQVTVNKEVSNVASNLLFLDRND
                     NLIISTALYFKEKYPDTKVILVSMDANVRIKADSVGLNAENFETGTVNFEELYTGCMI
                     KELPDDEFEALKDKPSIPNNFGNLYPNQFVRFTKKSDPSISHTMRYIHSSNTLIPFKY
                     YRGQEVFGIKSRNYEQEMALELLLDDNIKLVSLSGIAGTGKTLLAIAAGLQKVIEENK
                     YARLVISRPIFPLGKDLGYLPGSKTEKFNPWMQPIYDNMDILLSHHNQVGPANPKNKR
                     KTTIDDFMDYGYVELEPLTYIRGRSLPDQFMIIDEAQNLTPHEMKSIITRAGENTKIV
                     LTGDPYQIDIPYLDSSSNGLSVAVEKLKVEDIVGHITLIKGERSALADLAAKYF"
     gene            complement(3664..3751)
                     /locus_tag="PK015_06780"
     tRNA            complement(3664..3751)
                     /locus_tag="PK015_06780"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3715..3717),aa:Leu,seq:cag)
BASE COUNT         1207 a          753 c          661 g         1220 t
ORIGIN      
        1 tagaccttat ctataaagat ataaaaaaac cttaacaaat ttcgcaaaca gaattgaact
       61 tagataaagc aatcccataa accccacccc ttgtgtttaa taattatatc acccttacgg
      121 taaaggaaat tacaaggaca atgcttttcg cttttaccat acatttaaat aataagggac
      181 attgttcttt cttattttag atacaaggat tcatctataa aaaatcagaa agaataatgc
      241 attatttctt atattcctct ttttcaagtt cttttaatag gcaaaaacta ctccagattg
      301 agggaaaacc cattcttctg gtttagtggt cacagttatg atatttttag aagtggttat
      361 tttcggatat ttattttgac cggcgaaaaa gggaacgcat tctatctgtt tgagagtttc
      421 tggataggaa aagctaattt ggactccgtg agtaagttct gtgataaaga cacttaattg
      481 atgggaagat ttaggataga gggttaaggt attgatttca tagcgaactt cctgtccgat
      541 aaggttttta agtgcaggat gagaacagcg aatttccatt gcctctgatt tgaaatttat
      601 tctggtttcc agttctatat cattcacttt tacggaagaa agttgaaaag cagaaggatt
      661 aagctggaaa ttgccatcca taagccagcg atattccacc tcattccgtt tgaaatattc
      721 cttcaaagct ttatctgaat tggcacaggc aaccaggaaa aagtctcttt gtaaagtttt
      781 tgaaaagctt aaagtggtct ccgctttcca ataatcctta tctgaacttt cagtaaaaag
      841 aatatggtga cggaaatctt tacggtaacg aatatcccta tcctgattat aaaagaagac
      901 ccgcagaatt tctctaaaga tgagttcact catttcttcg ctattaattc tttgagcaat
      961 ggcagactgg ataatctgat tcaatttgga attaggatag tcactggaga caaaatccct
     1021 tctggataaa ggagcggcag taactaatct tcctccttcg taataatagc gcttagctct
     1081 gcgtcctacc tgacagagaa gttcatatga agaaccgtta tatagactta ccagattttc
     1141 tactctaata tcattgtgac cattgcctaa gaggataacc tcagttccat attgtatttc
     1201 aggagcatct gtgatgtcta cacaaatcat atccatactt acttttccga tgactggaca
     1261 gagtttgcct gcaataagca cttttccttt attagaaagc agaaaatcat aaccatcagc
     1321 ataaccaact gggattacgg catagcgtgt tttgcgtttt gctttccagg tttgattata
     1381 accaactgtt gcacccttgg gaatttcttt tatctgaatg atagtggatt tgaaggtcat
     1441 aacaggttta agttctatct tctccttctg agaaggatga gtgtagaccc cataacttaa
     1501 tatacctaac cgacaaagat ttccagaact tcccagactg ttaatcagag cagaactatt
     1561 agctatgtga atccattctg cttgaacttt atacttttta agaacttgtt taaatcgttc
     1621 ttcctgcatt ttactgaatt ctgggtcgct ttcggaagag gcaaaatggg aaaagacact
     1681 tttcacttct aattcagcag cattctgaca atatgaccag agttccgcaa aatctttttc
     1741 cagagcacca ctacgatgca tcccggaatc aaacttcaag ctaattggac atttaacttt
     1801 ttgtttagca caaaaggaga ccagagcttg cgcaaaagag agctcattga ccgtaggaat
     1861 aagtttatac tccagaatac ctggaatttc atctattaaa gaaggagaaa gaataagaat
     1921 gggagcttta caaccttcaa tgcgtaaaag ctttccttct tccagattag ctactccgag
     1981 agcggtggca ccttctttca aggcagcttg agttatttcc caggcaccat ggccataggc
     2041 atcagcctta acaacttgga taaacccttg ctctgatttc aaaaaagttt taagcgcttt
     2101 cagattactc cggaaagcat ccagatcaat ctctacccaa cttcgttcca gactttttcc
     2161 ttttttatcc aagattcaat cctaaaagta tttagcagct aagtctgcca gagctgacct
     2221 ttccccttta atcaaggtga tatgacccac tatatcttcc acttttagtt tttctacagc
     2281 aacagaaaga ccattggaag aagaatccag ataaggaata tctatctgat aaggatctcc
     2341 tgtgagaaca atcttagtat tttctccggc acgagtgatg atagacttca tttcatgagg
     2401 agtaagattt tgggcttcat caataatcat aaactggtca ggaaggcttc ttccacgaat
     2461 ataagttaaa ggttccagtt ctacataacc ataatccata aaatcatcta tagtggtttt
     2521 acgtttattc ttgggattgg caggtcccac ttgattgtga tgagaaagca atatatccat
     2581 attatcatag attggttgca tccagggatt aaatttttcc gtcttagaac caggtagata
     2641 tcctaaatcc ttccctaaag ggaaaattgg acgagagata accagacgag catatttatt
     2701 ttcttctatc accttttgta atcctgcagc aatagccaga agggttttac ccgttcctgc
     2761 tattccggaa aggctaacta acttgatatt atcatccagt aaaagctcta acgccatctc
     2821 ttgttcataa tttcttgatt ttataccaaa aacttcctgt ccccgataat atttgaatgg
     2881 aattaaggta ttggaagaat ggatatatct catcgtgtga gaaatagaag gatcggattt
     2941 ttttgtgaaa cgcacaaatt gattgggata aagattacca aagttattag gaatggatgg
     3001 tttatctttc aaggcttcaa attcatcatc cggaagctct ttaatcatac atccagtata
     3061 caattcttca aaattaaccg ttccagtttc aaaattttct gcattcaacc ccacactatc
     3121 agcctttata cgcacattag catccataga aacgaggatg actttggtat cgggatattt
     3181 ttccttaaaa tagagggcag tactgataat caagttatca tttctatcca gaaacagaag
     3241 gttagaagcc acatttgaaa cttctttatt aaccgtaact tgcaggattc cgccgttatc
     3301 taatttaaca ccttcttgca aacttccttt tgaccgtaag gcatccagat aacgacctat
     3361 ttgacgcgca tttctactct tctcatcaac accttttttg aactggtcta tttcttcaat
     3421 aacaataata ggaataacca ctctgttatc ctggaaggaa aatattgctt gaggattatg
     3481 caacaagaca ttggtgtcca gaacgaaaat cttttgagcc atatataacc tcttctttat
     3541 aactaaaagc ttttaatact tttctttaat aatagaaata aaatcaagag caaaaatttc
     3601 ttttatcaag aatagattta agggatttat cgtcaatgaa tttattagct gatgtacgaa
     3661 ttctggtgct gaagagggga ctcgaacccc tacgagctta tcgctcacta gtccctgaaa
     3721 ctagcgtgtc taccaatttc accacttcag ctcactcaac ttaattcaaa tctctaaccg
     3781 cagggtttgt gtcaagcaaa aataatgaat ttcacttcca agataaagct ggaggagttg
     3841 g
//
LOCUS       DAORZF010000070         3550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_17583_length_3550_cov_378.068383, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000070 DAORZF010000000
VERSION     DAORZF010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17583_length_3550_cov_378.068383"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PK015_06785"
     CDS             complement(<1..264)
                     /locus_tag="PK015_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34195.1"
                     /translation="MKRLSLLLVMIITVCLAFAGIDEYYTFNATTGTYTPITGTDASI
                     SSDDAISAAIPIGFTFPYGDNVYNEVKISSNGWIGLGTSQTSSN"
     gene            complement(540..1253)
                     /locus_tag="PK015_06790"
     CDS             complement(540..1253)
                     /locus_tag="PK015_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOL34196.1"
                     /translation="MEEQLRTLAQMQKLDDKIGALRQLQNELPKELNDILANVEQAEA
                     NLNTIKNEMEEITKKLRSIELDIKQYQENIKKYAQQLADIKTNKEYKALNSEIAYLKE
                     KISELESQELELMEEESECKEKMEKAKTELEEAKKIQQERENELRKKIASLDGDIEHL
                     RAERNKLAQTLPQSLVKQYAKLLKYKNNCAVAYARNGACGACGFVIRPQVRIEIQQRN
                     KINYCENCGRILMEKFEDL"
     gene            complement(1256..2077)
                     /gene="nadC"
                     /locus_tag="PK015_06795"
     CDS             complement(1256..2077)
                     /gene="nadC"
                     /locus_tag="PK015_06795"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOL34197.1"
                     /translation="MNWSEIIRLALQEDLGTGDITTDYLDLEPIPEKAFMIAKAPGIL
                     AGLEIVKEVFLTIDKNLKITMYKRDGDTVKRGDEILRLEGNPASILKGERTALNFLQR
                     LSGIATMTKAMVDKLEGTSAKLLDTRKTTPLLRSMEKYAVRVGGGYNHRFGLYDMILI
                     KDNHIRACGSITEAVKRVKEKNIAYKIEVEVSNIKELEEAVKAGVDRILLDNMSISKI
                     KNCVKRFGSKVELEVSGGVTLDNIREIAQTGVPYISSGALTHSYQSLDISLLFKE"
     gene            2409..>3550
                     /locus_tag="PK015_06800"
     CDS             2409..>3550
                     /locus_tag="PK015_06800"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HOL34198.1"
                     /translation="MKRMLFISLMMMLAITFLLGIPVNSDTATRVAKNFVLERMGSEY
                     AVTTAKLLDSVKGESYIYVVNLTPQGFILVAADDAAIPVLGYSSINNWGEVEIPVQLQ
                     DLLENWNAQLQDIREHNLTAPKETQALWSKYNCDSVLFVPDRSFRTVGPLLTTVWGQG
                     TYYNAMCPPDAAGPDGHVLTGCVATAMAQIMRYWSFPPVGNGSHSYNCPPYGTLSADF
                     GSTTYNWAAMPDRVTSSNPAVATICYHAGVAVNMQYGPNSSGAYSVDVPNALITYFKY
                     ASSAQFKEKTSYSSSNWENMMKGELDNSHPIYYSGSSSQSGGHAFVLDGYQVSDFHIN
                     WGWNGSYNGYYALTALNPGSENFTSDQAAVIGIIPTLPLPTLNEGF"
BASE COUNT         1013 a          667 c          594 g         1276 t
ORIGIN      
        1 gttactactc gtctgagaag tacctaaacc aatccagcca ttggatgaaa tcttaacctc
       61 attgtagaca ttatctccat aggggaaggt aaatccaatg gggatagctg cggaaatagc
      121 atcatcagag gaaatagatg catctgttcc cgtaatggga gtgtaggtcc ctgttgtggc
      181 attgaaggta tagtattcat caatacctgc aaaagccaga catacggtga ttatcatcac
      241 cagaagtaat gatagtcgtt tcataggtta actccttttt gtacatttat gttctttata
      301 taatcacttt caactggcta cagtataatc tctaacttta ttgttatagc cagtatcctg
      361 taatttattt aattttaatg tacatacatc agattctaca acactatgaa atcagcacta
      421 ttaatcaact atatttaata tattccattc ctcttttctg tcaagagata aattcacttt
      481 ttcttctttt tttataatta gcgggaaata tcttattttt ctggtcatta ttttatcgtt
      541 tataaatctt caaatttctc cattaaaatc ctaccgcaat tttcacaata atttatcttg
      601 tttcgttgtt gtatttcaat tcggacttga ggtcggataa cgaaaccaca ggctccacag
      661 gctccatttc gtgcataagc tacagcacag ttatttttat atttaagaag ttttgcatat
      721 tgtttcacca gggattgggg aagagtctgt gccaatttat ttctttctgc tctaagatgc
      781 tcaatatctc catccagaga agctatcttt tttcgcaatt cgttttctcg ttcttgttgg
      841 attttctttg cttcttcaag ttcagtttta gctttttcca ttttttcttt acattcagat
      901 tcttcttcca taagttccaa ttcttggctt tccaattcgg atatcttttc ctttagatag
      961 gcgatttcac tatttaaagc tttgtattct ttattggtct taatatccgc aagttgttga
     1021 gcatattttt ttatattttc ttgatattgt tttatatcaa gctctatgct tctcagcttt
     1081 ttggttattt cttccatttc atttttaatt gtattcagat ttgcttctgc ctgttccaca
     1141 ttagccagta tgtcatttag ctctttaggt aattcatttt gcagctgtct taaggcacca
     1201 attttatcat ctaatttctg catttgggct aaagtacgta attgttcttc catatttact
     1261 ccttaaatag aaggcttata tccaaagact gataggaatg agtgagagca ccgctggaaa
     1321 tataaggtac tccagtttgg gctatttctc taatattatc caaggtaact cctccagata
     1381 cttccagttc aactttagaa ccaaagcgtt taacacaatt tttaatttta gatatggaca
     1441 tattatccag aaggatacga tcaacaccag ctttaactgc ttcttccaat tccttaatat
     1501 tagagacttc aacctcaatt ttataagcaa tatttttttc ttttactctt ttaactgctt
     1561 cggtgatgct tccacaggct cttatatgat tatctttaat taaaatcata tcgtaaagtc
     1621 caaaacgatg attatatcca ccacctacac gaacggcata tttttccata gaacgcaata
     1681 aaggagtggt tttgcgggta tctaaaagtt tagccgaagt cccctcaagc ttatctacca
     1741 tagcttttgt catagtggct attccactta aacgttgaag aaaattaagt gcagttcttt
     1801 caccttttaa gatgctggca ggatttccct ctaatcggag tatttcatcg cctcttttga
     1861 ccgtgtctcc atcccgttta tacattgtaa ttttcaaatt tttatcaatg gtcaaaaaaa
     1921 cttctttaac aatttctaaa ccggcgagta taccaggagc cttggcaatc ataaaagctt
     1981 tttctgggat gggttctaaa tctaaataat cagtagtaat atcgccagta cctaaatctt
     2041 cttgtaacgc aagacggata atttcactcc aattcattta ccctccataa ataaaaaaaa
     2101 gcatttgcta tctggcaaat gctttaatat tcgttgttta tacttattta tagttattaa
     2161 ctgctctttt cgtatactca tttctctcac tctttctttc ataaatataa ctttaatttt
     2221 tatcttagcc attttgtcaa gtagaactta acttttaaaa attctaactt agccgataat
     2281 taagaaaaat ctgtgagtat atttaattgt aaaaataggt aaaaaataaa tcttgactta
     2341 atttgttaaa tattatacaa gaaagatagg tgacaaattc attatattcc ttttaaaagg
     2401 aggtaataat gaagagaatg ctattcatct cgcttatgat gatgttggca ataacttttc
     2461 ttttagggat accggtaaat tctgatactg cgacccgtgt ggcaaaaaac tttgttctgg
     2521 aacggatggg atctgagtat gcagtaacta cggcaaaact tttagattca gtgaaaggtg
     2581 aatcttatat ttatgtcgtt aacctaactc cgcaaggatt tatccttgtt gcggctgatg
     2641 atgcagcaat tccagtatta ggttacagtt ccattaataa ctggggtgaa gtggaaattc
     2701 ctgtccagct acaggattta ctggagaact ggaatgctca attacaagat attagagagc
     2761 ataatttaac tgctcccaaa gaaactcagg ctctctggtc taaatataac tgtgattcag
     2821 tgttatttgt gccggacaga agctttagga cggtaggacc tttgctcaca accgtatggg
     2881 gtcaaggaac ttattacaat gctatgtgtc ccccagatgc agctggtcct gatggtcatg
     2941 ttttaacagg ttgtgtagcc acagcaatgg ctcagattat gcgttattgg tcttttcctc
     3001 cagtagggaa tggttctcat agttataatt gtcctccgta tggaactctt tccgctgatt
     3061 ttggttctac tacctataat tgggcagcaa tgcctgatag ggtaaccagt agcaatccag
     3121 ctgtggcaac tatctgttat catgctggtg ttgctgtaaa tatgcagtat ggtccaaata
     3181 gctctggggc atatagtgtt gatgttccta atgctcttat cacatacttc aaatatgcaa
     3241 gctccgctca atttaaggaa aaaacctcat attcttcttc caactgggaa aatatgatga
     3301 agggagaact ggacaattct catcctattt attacagtgg ttcaagctcc caatcaggag
     3361 gacacgcctt tgtactggat ggatatcaag tttctgattt ccacatcaat tggggatgga
     3421 atggtagcta taatggttat tatgccctta ctgcgctgaa tccaggaagt gaaaacttta
     3481 ccagtgatca agctgctgtt ataggtatca ttcccacatt gcctttacct acattaaatg
     3541 aaggatttga
//
LOCUS       DAORZF010000071         3363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_18839_length_3363_cov_364.744256, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000071 DAORZF010000000
VERSION     DAORZF010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18839_length_3363_cov_364.744256"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            422..>3363
                     /locus_tag="PK015_06805"
     CDS             422..>3363
                     /locus_tag="PK015_06805"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HOL34199.1"
                     /translation="MKRSLLFILILGFITLGLASITEYYTFNQTSGTYNPFNPEEGNL
                     ISELSANNVLSSPISIGFIFPYGTNLYTEVKISSNGWLGLGSSQTNSLNYNQLDYIYN
                     CPILAPLWDDLSLQMGTCYYQIAGTAPHRVFTVQYTNLKWNYTASSFFNLQVKLYETG
                     KIEFIYGFATGAPNSPSASIGINMLPGGSEWFYSVTPGNPATVSTTTENNQISIWPGE
                     GTIYEFNPVIPFSNDLAAISISGNNVLTVGENSDYQILIRNSGNNDQNNYQIKLLLDD
                     EEIASLNGNPIQAGEFQTYTLSWIPSFNFIGPGNLSGKVVLSGDENPVNNQTSPLLIT
                     IQPAEIQEVTIGSGNELARIPIDFNKRNSLYECLYYPEEIGFSSGIIYSIKLYNNFVN
                     SPANGMTRIWLGTTDLPDLSLNWILSNQLTLVFEGNIDYPAGVNTISIPLQTPYLHYP
                     GNLVMMVQRPWDQQFYSENNNFYAQTIGSHRARKTNIDNQIFEPSSPPPSTVSGQFPK
                     TTFLYTNYSLLNDLSCLSINGNPAPSIGSNYYYTIAIKNNGIETQNNYVVRLMATDGT
                     ELSSVMGSTIAPQQTLEFDIPWIPAFEGSVSIYGEVILVGDEIETNNQSLPLKIKVYP
                     EGIFYSHITIGEGNLWERMPMDFYWKNSLFETVYLASELNTSGLLTDIQVYNYFYLNS
                     PNEPTKIWMGETTLNDLSQGWIPSTDLTLVFDGMVDYPAYNNDILIHLDTPYQYNGNN
                     LVVMFKRPWDPTYLHSDGVFRNQTGDVINRTLKVASDVIDYDPANPPANTTAQAIFPM
                     TTFMIKHTDPFGEPVTLPSSMNVACAVTIDGLPASSGDILAAYVDVNGVPQLRGKQTI
                     QLSDNLASCIIQIYTENDAEIVRFRIWDPNNNQIYLSSNTLSTIVNATIGSIPDNPFM
                     IYGISPQTQSLSLAGGWNLISLNISPPDHRIISLISAIANNVQQIKGTEGVYILNNPF
                     SSLAALTDG"
BASE COUNT         1029 a          629 c          559 g         1146 t
ORIGIN      
        1 atatcaaatt cttagctctt aaaaatcaag tatttcttcc atatatttca aaccttgata
       61 aattgaacta aaatttttaa aataaaactt gacagggaag atagataaat ttaaaatact
      121 tttgagaatt ttgaagataa aagctttgtg tttgagaaaa tatttatgct cctacaaagt
      181 gaaggtgtgg ttatgtataa aacagcgaga tggggaatac catctatcta aaaagccagc
      241 tttagtttaa agcatcctct catctcaatt agttattgaa ccgccatttt ctccgttctt
      301 ttgttatgtt ctcaaagttt ttatccagtt tatatagaag agagttaagc taaggataca
      361 gacagtacct gtataacctt gagttattga ttaactacta aacaagcaag ggagatttaa
      421 gatgaaaagg tctctattat ttattttaat tttaggtttt atcactttgg gattggcttc
      481 tataaccgag tattatacct tcaatcaaac cagcggaacc tacaatcctt ttaaccctga
      541 ggaagggaat ttgatatctg aattatcagc taacaatgtt ctttcttctc ctatttctat
      601 tggctttatc tttccttatg gcacgaactt atataccgaa gttaagatta gttcaaatgg
      661 ttggcttggc ttaggttctt cgcagacaaa tagcttgaat tacaaccaac tcgattacat
      721 ctataattgt cctattctgg cacctctctg ggatgactta agtttgcaaa tgggcacttg
      781 ttattatcaa attgctggta ctgctcctca tcgtgttttt accgtccaat acactaattt
      841 aaaatggaat tatactgcca gttcattttt taatttacag gtaaaattat atgaaaccgg
      901 gaagattgag tttatttatg gatttgctac cggggcacca aattctcctt ctgcttccat
      961 tggaatcaat atgctccctg gaggttctga atggttctat tctgttactc cagggaatcc
     1021 tgctacggtt tcaactacca cggaaaataa tcaaatctct atttggcccg gagaaggaac
     1081 tatatatgaa tttaatccag ttatcccatt ttccaatgat ttagctgcaa tctcaatttc
     1141 aggtaataat gtgcttacag tcggagaaaa ttctgattat cagatattga ttagaaatag
     1201 cggtaacaat gaccaaaaca attatcagat aaaactttta ctggacgatg aggaaattgc
     1261 ttccctgaat ggcaatccta ttcaagcagg tgaatttcag acctatacgc tttcctggat
     1321 tccttctttt aattttattg gtcctggcaa tttatctggg aaagttgttt tatcgggaga
     1381 tgaaaatcca gttaataatc aaacctctcc gttacttatt actattcaac ctgctgaaat
     1441 ccaagaagta actattggct caggaaatga attagccaga atacctatag attttaacaa
     1501 aagaaactca ctttatgaat gcctttatta tcccgaagag attggctttt ccagcggaat
     1561 tatctactct ataaagctct ataataattt tgttaattca ccagctaatg gtatgaccag
     1621 aatctggtta ggaaccacag atttgcccga cctaagttta aattggattc tttctaatca
     1681 attaactttg gtctttgaag gaaatattga ttatcctgct ggagtcaaca ccatctccat
     1741 tcccttacag acaccttatt tacactatcc aggtaatctg gtgatgatgg tgcaaagacc
     1801 ctgggaccaa caattttatt ccgagaataa caatttctat gcccagacta taggttctca
     1861 tagagctcgt aaaacaaaca ttgataatca aatttttgaa ccttccagtc ctccaccttc
     1921 tactgtttcc ggtcaatttc ctaaaaccac ctttttatac actaattatt ctctgctcaa
     1981 tgatctgagt tgcttgagta ttaatggcaa tcctgctcca tctattggct caaactatta
     2041 ttataccata gccataaaaa ataatggaat agaaactcag aataattatg tagtcagact
     2101 aatggctact gatggaacag aactgagttc tgtgatgggt tcaactatag ctccgcaaca
     2161 aacattagaa tttgatatac cctggattcc tgcctttgaa ggctctgttt ctatctatgg
     2221 agaagttatc ttggtaggag atgaaattga gaccaataat caatctcttc ctttaaagat
     2281 taaagtctat cctgaaggta ttttctatag tcacattact attggtgaag gtaatttgtg
     2341 ggaaagaatg cctatggatt tctactggaa gaactcctta tttgaaactg tatatcttgc
     2401 ttctgaactg aatacaagcg gtctattaac tgacattcaa gtttataatt atttttattt
     2461 gaattctcct aatgaaccaa ccaaaatctg gatgggagaa actaccctca atgacctttc
     2521 tcaaggttgg attccatcta ctgacctgac tttagttttt gacggaatgg tagattatcc
     2581 tgcctataat aatgatattt taatccatct ggatacccct tatcaatata atggaaataa
     2641 tctggtagta atgttcaagc gtccctggga tccaacttat ttacatagtg atggagtttt
     2701 tcgcaatcag acaggtgatg taattaatcg cacccttaaa gttgccagtg atgtcataga
     2761 ttatgatcct gccaatcctc ctgctaatac tacggcacaa gcgatatttc ctatgactac
     2821 ttttatgatt aaacatactg atccttttgg tgaacctgtc actcttccat ccagtatgaa
     2881 tgtagcttgt gctgtaacca ttgatggact tcctgcatct tctggagata ttctggctgc
     2941 ttatgttgat gttaatggag ttcctcagct aagaggtaaa caaactattc agttaagtga
     3001 taatcttgcc agttgtataa ttcagattta tactgagaat gatgctgaaa tagtccgttt
     3061 caggatctgg gacccgaata ataatcagat ttatctttct tccaatacgc tcagcactat
     3121 agttaatgcc accattggtt ccatccctga taatcctttt atgatttatg gaattagccc
     3181 tcaaactcaa tccttatcat tagcaggtgg ctggaatctt atttccttga atatatctcc
     3241 accggatcac cgtataattt ctttaatatc tgcaatagca aataatgttc agcaaattaa
     3301 aggaacagaa ggagtgtata ttttaaacaa tcctttttct tctttagctg ctttaacgga
     3361 tgg
//
LOCUS       DAORZF010000072         3272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_19502_length_3272_cov_383.172210, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000072 DAORZF010000000
VERSION     DAORZF010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19502_length_3272_cov_383.172210"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="PK015_06810"
     CDS             complement(<1..248)
                     /locus_tag="PK015_06810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOL34200.1"
                     /translation="MAAKPGKREQGQMIIGIGVDLVKIERVRKLLDNYTEFITQVFTA
                     GEIEFCRAKADSASSFAARFAAKEAMMKALGTGWNEKV"
     gene            complement(209..697)
                     /locus_tag="PK015_06815"
     CDS             complement(209..697)
                     /locus_tag="PK015_06815"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOL34201.1"
                     /translation="MNINNQVKYQQRGWVQYQNIKLEVETSGDYVQKVHFTSEVYPTI
                     NPSPTLKKVCQQLLEYLQGKRKEFTVKIALEGTAFQKKVWEAIQHIPYGETCSYKDIA
                     IAIGNPKAAIAIGQAARKNPVAIIIPCHRVIGANGDLKGYAGRIEIKEWLLNLEKGNK
                     DK"
     gene            complement(775..1128)
                     /gene="rplS"
                     /locus_tag="PK015_06820"
     CDS             complement(775..1128)
                     /gene="rplS"
                     /locus_tag="PK015_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOL34202.1"
                     /translation="MDILQTIGRDQIRTDLPEFRPGDTVKVYYKIKEGGKERIQVFQG
                     IVIQKRGSGISRTFTVRKVSNGVGVERTFPMNSPNIEKLEIVRYGQVRRAKLFYLRHA
                     KGKAARIKERKRFTA"
     gene            complement(1470..2153)
                     /gene="trmD"
                     /locus_tag="PK015_06825"
     CDS             complement(1470..2153)
                     /gene="trmD"
                     /locus_tag="PK015_06825"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOL34203.1"
                     /translation="MKIEVLSLFPECFKGFIESSMVARAINLGILKVDLINYRIFSQN
                     KHRQVDDYPYGGFPGMVIQAEPVYKAISDLVQKSYAPVIYFTPQGRKLNQSILKYYAT
                     LPRVILLCGHYKEIDQRLRNLAVSDEISLGDYVLSGGEIPAMAFIDGVSRLLPGVLSD
                     INSADSDSFSQEESTLGFPCYTRPEKWMELNVPEELISGNHQKIAQWAKNQSYRLTLT
                     RRPDLKSEK"
     gene            complement(2150..2653)
                     /locus_tag="PK015_06830"
     CDS             complement(2150..2653)
                     /locus_tag="PK015_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34204.1"
                     /translation="MQFSDLIGIGKLGGKEADGFYPVLVKSDYRPILLNIRDVYLIFN
                     SDRVFFVTISEIEETGRKLRIKFAEDGIDEERQKHREVKIAIPKQESSKSLPESVIGF
                     TAFFEGRNLGKITGFFHNNAQYVLEITDTQDEEILIPWVDHYIGKIDSDKEELTLLNM
                     NELLDIQ"
     gene            complement(2654..2884)
                     /locus_tag="PK015_06835"
     CDS             complement(2654..2884)
                     /locus_tag="PK015_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOL34205.1"
                     /translation="MKELIEFIVKALVDDPSSVDVKEIQGDKITLYELRVGKEDIGKV
                     IGKRGRTASAIRTILNAVSTKQGKRAELEIIE"
     gene            complement(2967..3191)
                     /gene="rpsP"
                     /locus_tag="PK015_06840"
                     /pseudo
     CDS             complement(2967..3191)
                     /gene="rpsP"
                     /locus_tag="PK015_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002852274.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
BASE COUNT          865 a          726 c          548 g         1133 t
ORIGIN      
        1 ttaacttttt cattccagcc agtacccaga gccttcatca tagcctcttt agcagcaaat
       61 cgtgcagcaa aagaagaagc tgaatctgct ttagcccgac agaattctat ttctccagct
      121 gtgaaaactt gagtaataaa ttctgtatag ttatccaaaa gcttgcgcac acgttcaatt
      181 ttaacgaggt caacgccaat tcctattatc atttgtcctt gttccctttt tccaggttta
      241 gcagccattc ttttatctct attctacctg catatccttt taaatcacca ttagcaccta
      301 taacacgatg acaggggata ataatggcaa caggattttt acgcgctgcc tgacctattg
      361 ctatggcagc tttaggatta ccaatagcaa ttgcgatatc tttatagcta caggtttctc
      421 cataaggaat atgttgaatt gcttcccaaa cttttttttg aaaagctgtg ccttctaaag
      481 caatcttgac tgtaaattcc tttcgctttc cttgaagata ctctaatagc tgctggcata
      541 ccttttttag ggtgggggaa ggattgatgg taggataaac ttcagaggta aaatgcactt
      601 tttgaacata atccccggaa gtttccacct ccagttttat gttctgatac tgcacccatc
      661 ctctttgctg gtatttaacc tgattattta tgttcatatc tgatatgctt tataaaacga
      721 gagccccgta ctcaaatttg ggagtgtggg gctctgattt taagtttttc gtaactaagc
      781 agtgaatctt ttgcgttctt ttatccttgc agccttacct ttggcatggc gtagatagaa
      841 aagctttgct cgacgaacct gtccataacg aactatttcc aacttttcta tatttggaga
      901 attcattgga aaagtgcgtt caacaccaac tccattggat actttacgca ccgtaaaggt
      961 tctggagata cctgacccac gtttctggat gactatccct tgaaaaacct ggattcgttc
     1021 tttgccgcct tctttaattt tataatagac cttcactgta tcaccggggc gaaattcggg
     1081 aaggtctgtc ctgatttggt ctctgccaat cgtttgcaga atgtccatgg ttcctcccat
     1141 atatattctc ggaagaaacg ctatcctttg tagccagttt aatcctgtgg tcaaattcac
     1201 ctttctgttc cgtatatctt ttaatttctc gcaggtataa cggaaacgaa acataactat
     1261 gtaatgtcat agcagtctga agttttttat taaactgttg tttaattatg gtaacttagt
     1321 tgaataagct ggaatttgtt atatttatcc ggctacaact tattcttgtg gaagcattac
     1381 ttcctgcgtt atttcagatt taaatgtagg tttcctgtgc tgttcctttc acagtagctt
     1441 ttttaattta cagtgagaat atggctaacc tatttttctg attttaagtc cggtcttcgg
     1501 gttaaagtta aacgatagga ttgatttttt gcccattggg ctatcttttg atgatttcca
     1561 gaaattagtt cttcagggac atttaattcc atccattttt cagggcgggt ataacaggga
     1621 aatccaagag tgctttcttc ctggctaaag gaatctgaat cagcagaatt aatatcactt
     1681 aaaacaccag gtaaaagtct tgatacacca tctataaatg ccatcgcagg aatttctcct
     1741 cctgaaagaa cataatctcc aagagagatt tcatcactaa ctgccagatt tcgcaatctt
     1801 tgatctatct ctttataatg accacatagc aagattactc gtggcaaagt agcatagtat
     1861 ttcaggatag attgattcaa ttttcttcct tgaggagtaa aatatatcac cggagcatat
     1921 gatttttgca ctaaatccga tatggcttta tacaccggtt ctgcttgaat gaccattcct
     1981 ggaaagcctc cataaggata atcatccacc tgtctatgct tattctggct aaaaatacga
     2041 taattgatta ggtctacctt cagaatacca aggtttatcg cacgagctac catactgctt
     2101 tctataaatc ccttaaagca ttccgggaaa aggcttaaaa cttcaatttt cattggatat
     2161 ccagcagttc attcatatta agcaatgtca gctcttcttt atcgctatca attttgccaa
     2221 tataatgatc cacccaggga attagtattt cctcatcctg ggtatctgtt atttccagca
     2281 catattgagc attattatga aaaaaacctg tgattttacc cagatttctg ccttcaaaaa
     2341 aagcggtaaa acctatcacc gattcaggta aagatttact gctttcctgc ttaggtatgg
     2401 ctattttgac ttcccgatgt ttctgccgtt cctcatctat tccatcttcg gcaaacttta
     2461 tccttagctt ccgaccggtt tcttctattt cactgatagt cacgaaaaac actctatcac
     2521 tgttaaagat caaatacaca tctctgatat ttagcagaat agggcggtaa tcagatttta
     2581 ccagcacggg ataaaatccg tccgcctctt ttccgcctaa tttgccgatt ccgattaaat
     2641 cggaaaactg catttactca ataatttcta attcggctct ctttccttgc ttggtgctta
     2701 ccgcattaag aattgtgcga atagcacttg ccgtgcgtcc tcttttgccg attacttttc
     2761 caatgtcttc ttttccaacg cgtagctcat aaagagtaat cttgtcacct tgaatctctt
     2821 tgacatctac actgctcgga tcgtcaacta acgccttcac gatgaattca atcaattcct
     2881 tcatttttca cctcttcttc tttacttctt tttgcagcag cttgtgcttc taccttttgc
     2941 atatgccgca tctgtaaaat tcctgctttg cgtaagagag accttactgt atcggaaggt
     3001 tgagctccaa cgctaagcca atacaaggca cggtccttat caatctgaat tcttgcgggt
     3061 gtaggcttcg gatcatacca ccctatgctt tctatatatt tcccgtctct acttacacga
     3121 gaatctatag ctactatacg gtaaaagggc tggtcaattg cacccatccg cctcaatctc
     3181 agcttgacca ttttttctcc ttctttcaat ttatcaatgc tttatttttt atcatctatt
     3241 tttcgtcaag atttttcttt ccgtgcctca ca
//
LOCUS       DAORZF010000073         3205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_20032_length_3205_cov_336.971429, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000073 DAORZF010000000
VERSION     DAORZF010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20032_length_3205_cov_336.971429"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1121)
                     /gene="murJ"
                     /locus_tag="PK015_06845"
     CDS             complement(<1..1121)
                     /gene="murJ"
                     /locus_tag="PK015_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOL34206.1"
                     /translation="MAENKLIKNISVMSFAILISRIMGLVRDQVMAFCFGTTYLNDAF
                     VVGYRIPNLLRSMFGEGALSAAFVPLYNEIGIKEGKRKQINFALQVLSILTFFLLILT
                     IIGIIFAPAIVKVLYPGLLPKTYKLAVILTRIMFPYLLWIGLSSTMIAILNSHDKFFM
                     TGLSSALLNLGMILSMVIPRFVFHIEGEDLVRWAAVGVFGGGFLQTIINFPYLKQLGY
                     PVKIMVKFGGEALNTLWKRFLPSLVGVGVRELNLVADSLMASFLAVGSISALEYGYRL
                     FQFPLGMFAISTGTVLLPTYSRLVTYDNYPELSKNLRIAMLNLTYLMLPITVLLMGLG
                     KDVVQILFQRGAFDAKATLWTTQALICYSLGLIFFSFNQT"
     gene            complement(1126..2265)
                     /gene="holB"
                     /locus_tag="PK015_06850"
     CDS             complement(1126..2265)
                     /gene="holB"
                     /locus_tag="PK015_06850"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HOL34207.1"
                     /translation="MQEQSLFQKIRGQSRALKILFQAIENEHIAQAYLFHGIEGVGKF
                     TTALYFGMALNCLANKEFRPCGVCSSCHKFLNFEHPDLLYLFPTPNLQLTPEGEIKND
                     EYRKEYEGYIRNKIETPWEDYRFSSNIMIRKESISWLIKRLNISSYEAKYRICIIEDA
                     DLMNVETANAFLKTLEEPPESTVIILTTNNPSQLLPTIVSRCQPIHFQPVDPNVIAQI
                     LIDDFNTSPRTARIVSQIADGSVKYAIHLVQGESSKLRERAFEIMQMALEGNDLAYYR
                     SLQANLQKYNAENLIEIIRYLCMFVGDLDLIESAEEQVVNIDKKDFLIEARAKIPIQN
                     SAELTDKVLDFVLLMQDYIRKIRGNVNLQLIQQNLYFQLNKLFRP"
     gene            complement(2287..2892)
                     /locus_tag="PK015_06855"
     CDS             complement(2287..2892)
                     /locus_tag="PK015_06855"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOL34208.1"
                     /translation="MKYFRKMSGEKCYLSPISLDDVELYTQWVNDMEIGQFVLFSTTI
                     YDIEKEKEALKKLMAESISFAIVEKNTNKAIGNCSLFGTSEVHRHGQFGIFIGDKSYW
                     SQGIGTEATRLILDYGFNVLNLNNISLEVYAFNKRAIRCYEKVGFKLAGRRRNYLFMA
                     GKYHDVLIYDILAEEFESPYVNKLFERSSNEDTEAKKISWI"
BASE COUNT         1098 a          584 c          532 g          991 t
ORIGIN      
        1 gtctgattaa agctgaaaaa gataagacct aaactataac agatcagagc ttgagttgtc
       61 cagagagttg ctttagcatc aaaagcacca cgctgaaaaa ggatttgcac cacatcttta
      121 cccagtccca ttaaaagaac agtaattggt agcatcagat aggtgagatt gagcattgct
      181 atacgtaagt tttttgaaag ttctggataa ttatcgtaag taaccaatct ggaataagta
      241 ggtaataaaa cagttccagt ggaaatagca aacattccca gtgggaattg aaacaatcta
      301 taaccatatt ccagagcaga aatacttcct acagcaagaa aggaagccat taaagaatcc
      361 gccacaagat tgagttcacg aactccaact ccaactaatg atggtagaaa ccttttccat
      421 aaagtattta gagcttcacc tccaaattta accattattt tgacaggata acccaattgt
      481 tttagatagg gaaagtttat gatggtctgt aaaaaacctc ctccgaaaac accaacagca
      541 gcccaacgaa caaggtcttc gccttctata tgaaagacaa atctcgggat taccatactt
      601 aatatcatac ctaaattcag caacgcagag gataatccag tcataaagaa tttatcgtga
      661 gaatttagta tggcaatcat tgtggaagaa agtccaatcc agagaagata agggaacatt
      721 attctggtca ggataacagc taatttataa gttttaggaa gcagaccagg ataaagtact
      781 tttacaatag caggggcaaa aatgatacca ataatggtta gaatcaagag gaaaaatgtt
      841 agtatggaaa gcacttgcaa cgcgaaatta atctgttttc ttttcccttc tttgattcca
      901 atttcattgt ataaagggac aaatgcagcg gaaagagcac cttcaccaaa catactacgc
      961 aacagattcg gaatgcgata accaactaca aaagcgtcat ttagataggt agtcccaaaa
     1021 cagaaagcca taacctgatc acggactaat cccataatcc tgctaatcag tatagcaaag
     1081 gacattacac tgatattttt gattaattta ttctccgcca tagcttcagg gacggaaaag
     1141 tttatttaat tggaaataga gattttgttg tatgagctga agattaacat tgccacggat
     1201 tttacggata taatcctgca tcagcaatac aaaatccagc actttatcag ttaattctgc
     1261 actattctgg atgggaattt ttgctcttgc ctcaataaga aaatccttct tatcaatatt
     1321 tactacctgt tcttcagcag attctatcaa atccaggtct ccaacaaaca tacaaagata
     1381 gcggataatc tcaataagat tttctgcatt atatttttgt aaattagcct gtaaagaacg
     1441 atagtaagct aaatcattac cttcaagagc catttgcata atttcaaaag ctctttcacg
     1501 aagttttgat gattctcctt gaaccaaatg tatggcatat ttaacgcttc catctgctat
     1561 ttgggagact attcttgctg tcctgggaga agtgttaaaa tcatctatca atatctgagc
     1621 aattacattg gggtccacag gttgaaaatg tataggttga cagcgtgaaa ctattgtagg
     1681 tagtaactgc gaaggattat tagtagttaa aattataaca gttgattcgg gaggttcttc
     1741 cagagtttta agaaaagcat ttgccgtttc tacattcatt aaatctgcat cttcaataat
     1801 acaaatccgg tatttggctt cataactgct gatatttaag cgtttaatca gccaggaaat
     1861 actttcctta cgaatcataa tattagagct gaaccgatag tcttcccagg gtgtttctat
     1921 tttattacgg atgtaacctt catattcctt acgatattca tcatttttta tctcaccctc
     1981 tggagttaat tgaagattgg gagtagggaa aagatagagt aaatcaggat gttcaaaatt
     2041 aagaaattta tgacaggaac tacaaactcc acagggacgg aattctttgt tagccagaca
     2101 atttagagcc ataccaaagt ataatgcggt agtgaatttt cccactcctt ctattccatg
     2161 aaaaagatag gcttgggcta tgtgttcatt ttctatagct tgaaaaagaa tttttaaagc
     2221 tctgctttga cctcttattt tttggaataa actctgttct tgcattttaa caaagctgga
     2281 aaaaccttaa atccaggaaa tcttttttgc ttcagtatcc tcattagaac tgcgttcaaa
     2341 aagtttatta acatagggac tttcaaattc ttctgctaaa atatcataga ttaaaacatc
     2401 gtgatattta cctgccataa agagataatt acgacgacgt ccagctaact taaaaccaac
     2461 tttttcataa caacgaatgg ctcttttgtt gaacgcataa acttccagac ttatattatt
     2521 taaatttaga acattaaaac cataatctaa tataagcctg gttgcttctg tacctatgcc
     2581 ttgactccag tatgacttgt ctcctataaa aataccaaat tgaccatgac gatgtacttc
     2641 acttgttcca aaaagactgc aattaccaat tgccttattc gtgttcttct ctacaattgc
     2701 aaaacttatg ctttctgcca ttaacttttt taaagcttct ttttcttttt ctatatcata
     2761 aatagtagtg gaaaacaaga caaactgacc tatctccata tcattgaccc attgtgtata
     2821 aagctctaca tcatctaaag aaatggggga aaggtaacat ttttctccgc tcatttttct
     2881 aaaatacttc attttgttct cctttattat tttaatggtt aggattgctt attcttcaaa
     2941 tgatttaaac tacttaataa gcactatctg tcaagataaa tccgtaactt atttagtaaa
     3001 ataaagatga ggatttgaaa tgactgttcc aggaaagttc agtggtaaaa tattaaacca
     3061 gaaaaaagtt agacattgaa agctgaaact ttgatccaga ttggagacct aaagaaaatg
     3121 taaaacctat atctaactga tgtccacttt cggacataag ctggacatga gttggacata
     3181 agctggacat aagttagtca taagc
//
LOCUS       DAORZF010000074         2915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_22691_length_2915_cov_370.020629, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000074 DAORZF010000000
VERSION     DAORZF010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22691_length_2915_cov_370.020629"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..2690)
                     /locus_tag="PK015_06860"
     CDS             complement(159..2690)
                     /locus_tag="PK015_06860"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOL34209.1"
                     /translation="MKAHFQCFLIGFIVLAFFVLPISLIANNNKSALSDRTQISVTIG
                     AGNLVGRVPVDMSYRTSLFETIYLATEMNAAGTITDIMFYNNFESNLTDKPTQIWLGE
                     TLSENLTNGWIPASQLTQVFADNVDYPSGINTITIHLTNPYNYRGGNLVMLVYRPWEE
                     DYYMNQDLFACQINGDNRSLVTYNDNTQINPDNPPTTGVIGLFPKTTFLINVDGMGSL
                     SGNVSSSGIPLEGARVQIEDSPLYRITGANGNYSFPYIQPGTYTITASKLGYNPQSHT
                     VTILAGQSATQNFALTGIPEVTVEGRVVGSDAPNVGIANATVSFTGYADYTTVTDAQG
                     YFIFYSVYANQTYDYTITAPGYQIYTGQAVVGSSNLDMGEIVLSEMAYSPINVIATEI
                     NNGTAVQISWQEPLVPEEGWIHYDSGQNFNAFGTAGSANFEVAARFPADSLAAYAGGY
                     LQAVKIWPAMGGNFVVHIWLGGNALGPGPMVLSQPIIPVLNTWNTVILNTPILITGTE
                     ELWIGYLCDTTGFNPAYAGFDEGPAVNGFGNMILWQDQWTTLLAVNSYCDFNWNIQGY
                     VGLTPPALSTYAPEINLNASNDRSMIGFKVWRLLQGQANNEDFWVSLNEQPTTDLSII
                     DTAWNNLPDGTYLWAVKSVYTGGVMSDPAFSNSLTLITPVGTIAGFVRSTSNVPIGGA
                     TVTCENVSVTTNTAGAYTMLVAAGIHSVTASHPNYQSQTQEGVLVQVGLTTTLNFFLQ
                     ETAIQDDNIPVVRTELLGNTPNPFNPETIIYYNIKESTPLKINIYDMKGQLVKTLIDE
                     VKTPGHYKVVWDGKDNHNQPVSSGVFFYQMQCKNYSCTKKMLLLK"
BASE COUNT          926 a          560 c          520 g          909 t
ORIGIN      
        1 tcaaggttaa atagctatcc tacttagact gtgatttaaa gtaatatttt cttttggatt
       61 attttgtaaa atttatggat ctatcagttt acaacctaaa tataaaaggc ggagtttaga
      121 gaaaaatgaa ctccgcctta atgtaaatat tcttaacttt atttgagcaa caacattttt
      181 ttagtgcagc tatagttttt gcattgcatc tgatagaaaa agactccact ggaaacaggc
      241 tgattatgat tgtctttccc atcccagaca actttataat gtccaggagt tttaacttca
      301 tctattagag tcttaactaa ctgtcctttc atatcataga tattaatttt caaaggagta
      361 ctttccttaa tattataata gattatagtt tcgggattga aaggattggg tgtatttccc
      421 agaagttctg ttctgacaac tggaatatta tcatcctgga tggcagtctc ttgcagaaag
      481 aagtttaagg tggtggtcaa tcctacctga accagaactc cttcctgtgt ttgagattga
      541 taatttggat gacttgcagt cacactatgt attccagctg caaccagcat agtataagct
      601 ccagctgtat tggtggtaac gcttacattt tcacaagtca cagttgctcc acctatagga
      661 acattagaag tgctacgcac aaaacctgct atagtaccca ctggtgtgat taaagtgaga
      721 gaattggaaa atgctggatc ggacatcact ccacctgtat aaactgattt gactgcccat
      781 agataagtac catcaggaag attattccag gctgtatcta ttatagataa atctgtcgtt
      841 ggttgttcat taagactcac ccagaaatct tcattatttg cctgtccctg aagtagtcgc
      901 catactttga atccaatcat acttcggtca ttagatgcat ttaaatttat ttctggagca
      961 taagttgata atgcaggagg agttaatccc acataaccct gaatgttcca gttaaagtca
     1021 caataagaat ttactgctag taaggtcgtc cattgatcct gccagagaat catattccca
     1081 aaaccattta ctgcgggacc ttcatcaaat ccagcatagg caggattaaa acctgtggta
     1141 tcacaaagat aaccaatcca gagttcctcc gttccagtga taagaatggg tgtatttagt
     1201 ataaccgtgt tccaggtatt taatacagga ataataggtt ggcttagaac cataggacca
     1261 ggacccagag catttccacc taaccagatg tgcactacaa agttaccacc catagcaggc
     1321 caaatcttga cagcttgtaa ataaccacca gcataagcag ctaaagaatc agcaggaaaa
     1381 cgagcagcta cttcaaaatt agcagaacca gcggttccaa atgcattaaa gttttgtcct
     1441 gaatcataat gaatccaccc ttcttcgggg actaaaggtt cctgccaact tatctgtacc
     1501 gctgttccat tattaatttc agtagcaata acatttattg gactatatgc catctcgctt
     1561 aaaacaattt cacccatatc taaattggaa gaaccaacca ctgcttgtcc agtataaatc
     1621 tgatatcctg gtgctgttat agtatagtcg taagtttgat tagcatagac actataaaat
     1681 atgaaataac cctgagcatc ggtaacagtg gtataatctg catatcccgt aaaagaaacg
     1741 gtagcgttag caataccaac atttggtgca tcacttccta ctactcttcc ttccacggtt
     1801 acttccggga taccggtaag agcaaaattt tgagtggctg attgacctgc aagaatagta
     1861 actgtatgag attgaggatt ataacctaat tttgaggcag taatagtgta tgttcctggt
     1921 tgaatataag ggaagctata attgccattt gctcccgtga tacgatataa tggactatct
     1981 tcaatttgca ctcttgcacc ttccagaggt ataccagaag agctaacatt cccactcaaa
     2041 cttcccattc catccacatt aattagaaat gtagttttcg ggaaaagacc gatcactccc
     2101 gttgttggag gattatcagg attaatctgt gtattgtcat tataagtaac cagagaacga
     2161 ttgtctccat taatttgaca ggcaaataaa tcttgattca tgtaataatc ctcttcccag
     2221 ggacgatata ccaacattac cagattgcct cctctataat tataaggatt agtaagatgt
     2281 atagtaatag tatttattcc tgaagggtaa tctacattat ccgcaaaaac ctgggttaat
     2341 tgggaagctg gaatccaacc atttgttaga ttttcggaaa gtgtttctcc taaccaaatc
     2401 tgggtgggtt tatcagttaa attactctca aaattattat aaaacattat atctgttatt
     2461 gttcctgcag cattcatctc cgtagcaaga taaatggttt caaataaact tgttctatag
     2521 gacatatcta ccggaaccct gcctacaagg ttgccggctc caatagttac tgaaatttgg
     2581 gttctatcag aaagagccga tttattgtta ttggcaatca aactaatagg tagaacaaaa
     2641 aatgccaaaa ctatgaatcc tattaagaaa cattgaaaat gtgctttcat cttcattctc
     2701 ctttattaaa cttaatctga tagatatgca attattgaac caatgtggat tcaatgtgaa
     2761 agaaaatggt catatattta aaaactttta gtatatagag gaaagaaaaa gccccggaag
     2821 gtaagacctc tccggggcta ggagttatgg taggaattgt ttatttcatc ataatcattt
     2881 tcttagtgct gctgtattta ccagcattca tctta
//
LOCUS       DAORZF010000075         2779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_24206_length_2779_cov_384.926946, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000075 DAORZF010000000
VERSION     DAORZF010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24206_length_2779_cov_384.926946"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            277..1260
                     /locus_tag="PK015_06865"
     CDS             277..1260
                     /locus_tag="PK015_06865"
                     /inference="COORDINATES: protein motif:HMM:NF013895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOL34210.1"
                     /translation="MKKKDKFAQHSIHELMHTDFIALKEDLSVGEALEQIRQCAGEPR
                     IMYFYVVDEKDMLKGVVSTRQLLISPLDAKISEIMDTKVHYLTEEVNIMDVLNDFITH
                     KYLALPVVDKEERIIGVVDVSVFTENIPDILERENSEAVFETIGFRIYQIKGASAFQA
                     FRYRFPWLIATITSGVLCALMSSRFENVLASSLILSFFLTLVLGLGESVSMQSMTVTI
                     QALRVQHPTFKWFSREIIKEFGTAILLGLASGAAVAASVLIWKQNVKAALVIGGSLVF
                     TLTAACLIGLAIPTTLHALKLDPKVSAGPLTLGIADIITILTYLTFASVIL"
     gene            1463..1804
                     /locus_tag="PK015_06870"
     CDS             1463..1804
                     /locus_tag="PK015_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34211.1"
                     /translation="MSKKHYEIVAELDPAEVKNVPEVHKKHYSELGYKPYRMGDGRVK
                     WLTDAEKVYTETKSAVKSFGLKKSSSSVSHRKSRHKRRHRNRFYAFISDNWFIFIIIA
                     LAIFVYLFFRL"
     gene            1826..2608
                     /gene="surE"
                     /locus_tag="PK015_06875"
     CDS             1826..2608
                     /gene="surE"
                     /locus_tag="PK015_06875"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOL34212.1"
                     /translation="MRILLTNDDGINALGLRMLAEYLNKAGHQVIIVAPDRERSASSH
                     SISLKKNLRLTRIREDEYCVDGTPVDCIVVATQKILQRPVDLVISGINAGQNMGEDVL
                     YSGTVAAALEASLFGYKAMAVSINSYQNQNFDTPASWMVKLLDLGLDTLIPGNGILNL
                     NFPNVPSEELRGIRLTRTGHRRYYNFIHLIEELDDGFIYRIGGEAPAWEIESGTDAEA
                     IAENFISITPLGLSLTLGDAFPSILQWLESNQLLYFERSDAI"
     gene            2598..>2779
                     /locus_tag="PK015_06880"
     CDS             2598..>2779
                     /locus_tag="PK015_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOL34213.1"
                     /translation="MRFEDQRTLLVQELASEGITDERVLDAFRKVPRELFVPSQFREY
                     AYRNQPLPIDCYQTIS"
BASE COUNT          868 a          453 c          566 g          892 t
ORIGIN      
        1 caccaaaaca cattcatttt attttataag ttatttttat tgcttattac gcaactgttt
       61 taatatctat ttattaccaa accattagag ttaaagaata atttgggtta atttattctg
      121 taggggaaaa ttctttataa gacattaaat tctgatgatt aaaaattgag acagacttca
      181 taattcctcc aaactttctc ttgtcattta ttcttcagta ataaaaattg ttttttagtt
      241 attaatttac atcaattcaa taaattggag acagggatga agaaaaagga taaattcgct
      301 cagcactcaa ttcacgaatt gatgcacaca gattttatag ctctgaaaga ggacctaagt
      361 gttggagaag cattagaaca gattcgtcaa tgtgctggtg aaccgaggat aatgtatttc
      421 tatgtagttg atgagaagga tatgctgaaa ggagttgttt ccaccaggca gttattaatt
      481 tctcccttag atgccaagat ttcagagata atggatacta aagttcacta tttaacagaa
      541 gaagtgaaca ttatggatgt attgaacgat tttataactc ataaatatct ggctttacca
      601 gtggtagata aagaagagag aatcattggc gtggtggatg tgagtgtttt tacagaaaac
      661 ataccggaca ttctggagag ggaaaattct gaagctgtat ttgagaccat tggtttcaga
      721 atatatcaga ttaagggtgc atcagctttc caagcttttc gctatcgttt cccctggtta
      781 atagcaacta ttaccagtgg agttttatgt gctttaatgt cttcgcgttt tgaaaatgtt
      841 ttagcttcca gtttaattct ttcatttttt ttaactcttg ttctgggtct aggagaaagt
      901 gtaagtatgc aatctatgac tgttaccatc caggctttaa gagtgcaaca tccaaccttt
      961 aaatggttca gtcgggaaat aattaaagaa tttggcacag ctatattact tggtctggca
     1021 agcggagctg ctgtagctgc ttcagtttta atctggaagc agaatgttaa agcagcttta
     1081 gtaattgggg gttccttagt ttttactttg accgcagctt gtttaatagg actggcaatt
     1141 ccaactactt tgcatgcatt aaaactggac cccaaagttt cagctggacc cttaactctt
     1201 ggaatagcgg atatcataac catattaacc tatttgacct ttgcctcagt tatcctttaa
     1261 gaatgattaa tgcggtcttg cgctgatttg aaacttaatt taaaggtcta ttataaagat
     1321 aaagactact atctctcaat atacaattcc aaatccgtat tatacactct taatttgttc
     1381 aaatcggaaa aaaatagtgc cttcagatag tcattgacag aaaggattat aagaaatata
     1441 atgttttaat aaggtaaaga atatgagtaa aaaacattat gaaattgtag cagaacttga
     1501 cccagcagag gtgaagaatg tgccagaagt gcacaaaaag cattattccg agctgggata
     1561 caaaccttac aggatgggcg atggtagagt caaatggtta accgatgcgg aaaaggtcta
     1621 caccgaaacc aaaagtgcag tgaaatcttt tggtttaaaa aagtcttctt cttccgtaag
     1681 tcatagaaag tccaggcata aacggaggca ccgcaataga ttttatgctt ttatatcgga
     1741 taactggttt atttttatca taattgcact tgccatattt gtttatttgt ttttccgttt
     1801 ataaaaaaca cataggaata gagaaatgag aattctactt actaatgatg atggaattaa
     1861 tgccttaggt ttgagaatgt tagcagaata tctgaacaag gcaggacatc aggttattat
     1921 tgttgcgcca gatagggaac gcagcgcatc ttcccattct atctctttga aaaagaattt
     1981 gagattgacc aggattagag aggatgaata ttgtgtggat ggcacacccg tggattgcat
     2041 tgtggtggct acacaaaaga ttttacagcg tcctgtggat ttggttatat ccgggataaa
     2101 tgcagggcag aatatgggag aggatgtgct ttattcaggg actgttgccg ctgctttaga
     2161 agcaagttta tttggctata aagctatggc agtatccatt aattcctatc agaatcagaa
     2221 ttttgatact ccggcaagct ggatggttaa gcttttggat ttgggtctgg atactttaat
     2281 tcccggaaat gggattttaa atctgaactt tcccaatgtg ccatccgaag aattaagagg
     2341 aataaggctc actcgtaccg gtcaccgaag atactacaat tttattcacc ttattgaaga
     2401 gctggatgat gggtttatct atagaatagg tggagaagct ccagcttggg aaattgaatc
     2461 gggcacagat gccgaggcta tagctgagaa ttttatttct atcacacctt taggtttgag
     2521 tttaactctt ggtgatgctt tcccttctat tttacaatgg ctggaaagca accagttact
     2581 ctattttgag agaagtgatg cgatttgaag atcagcgaac acttttagtt caggaattag
     2641 ccagcgaagg tatcacggat gaacgagttc tggatgcctt ccgaaaagtg ccacgcgaac
     2701 tttttgtccc atcacaattt cgtgaatatg cctaccgaaa tcaaccttta cctatagatt
     2761 gttaccaaac aatatcaca
//
LOCUS       DAORZF010000076         2733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_24800_length_2733_cov_374.504108, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000076 DAORZF010000000
VERSION     DAORZF010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24800_length_2733_cov_374.504108"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="PK015_06885"
     CDS             <1..743
                     /locus_tag="PK015_06885"
                     /inference="COORDINATES: protein motif:HMM:NF027451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HOL34214.1"
                     /translation="RVSTHITSTLAPDYINLVRKNGAEMFVVNNFYVRDQLIIYLVAK
                     DAPTLKNLTKERSEQVFNYFLDRYKQRLAYQVYFNEVISDDYFADWPFTIKIPVLYMV
                     FKDDKPGHFLSFIYQPAKQDTKHPDKYVSIYYESMPENRITNEWIYNTRQMLGKKYLN
                     GDEIYDNAYTIEKAKIAEYDGLKMYGHWINKELSGGVGGAFQSWAFWDARTKTAYLID
                     NIVYMPAVTYKLPYLLELEMISHSISIK"
     gene            740..2467
                     /locus_tag="PK015_06890"
     CDS             740..2467
                     /locus_tag="PK015_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34215.1"
                     /translation="MNHFFRHILLLCLFLLALSSGASKEIDKESTNIIPDYRAIKSYT
                     LASYYYFDGDYVAADKYFKQSLKYDPKSYTIRKQSLLNSFELYNKKGISAKKLQKLID
                     DYKKFAPLDEDLLYAAYDFYRYTQNETELIKIISELQTKYPSPRAYLQSFLFELQYHQ
                     RVYPEYLSKAQELAGNDPTFYPLLIRLWSFFSPEMEKQAIIRYYELDKGEQSNAFLAE
                     YIVRNQDLELAQQYLSSLHYPEDKDYMLYFATAQWSNQPNPLLPDLADYFLRTSDLEL
                     ISELALSALTQHRWDLFPEIELTVEASSASDEEKQSLYSLLIAYSLYTSNDKNLADIL
                     EKLTELKYFDAVFIYYSCAVNQKIDPNVKLPTAEDYELFINEIKKRLPDEPPARYLQT
                     IASPEEENSEPLILDSKYLLALWLKEHHTLCPEDYEFIISYCHLKKNFSEERNFLLEA
                     INKWPDYPSFYNDLGYSMLINGEDPEQAATLIRKALTYEPENPYFLDSLAWYNYLTGN
                     YSEALNLMSIPQKMENMPAEIAYHLGAIHLALHNYKDAHLWLQKCLTINNDPEAEELA
                     IKALKQIPK"
     gene            2656..2730
                     /locus_tag="PK015_06895"
     tRNA            2656..2730
                     /locus_tag="PK015_06895"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2688..2690,aa:Asn,seq:gtt)
BASE COUNT          893 a          556 c          411 g          873 t
ORIGIN      
        1 atcgggtctc cactcatata acttcaactc ttgctcctga ttatatcaat ctggtacgga
       61 aaaatggtgc agaaatgttt gtagtcaata atttctatgt acgagaccag cttataatct
      121 atttagtagc taaagatgct cccaccctaa aaaatctgac aaaagagcga agtgaacaag
      181 tttttaacta tttcctggac cgctacaagc aacgtctggc ttaccaggtt tatttcaatg
      241 aagtaatctc tgatgattac tttgccgatt ggcccttcac tattaagata ccagttcttt
      301 atatggtttt caaagatgat aaaccaggtc atttcctctc ttttatctat caaccagcta
      361 aacaagacac caaacatcca gataaatatg tctctattta ttacgaatct atgccagaaa
      421 atagaattac taatgaatgg atatacaaca cccgtcagat gttgggcaaa aaatacttaa
      481 atggggatga aatctatgat aatgcataca ccatagaaaa agccaagatt gcagaatatg
      541 atggactaaa gatgtatgga cactggatta acaaggaact atctggagga gttggtggag
      601 ctttccaaag ctgggctttt tgggatgctc gcacaaaaac tgcttatctg atagataata
      661 ttgtctatat gccagctgtc acctataaac ttccctatct gcttgaactg gaaatgatat
      721 cccattctat atctattaaa tgaaccattt ttttaggcat atactacttt tatgcctctt
      781 cttgctggca ctttcttcag gtgccagcaa ggagattgat aaagaatcca caaatataat
      841 tccggattat cgtgccatca aatcctatac cctggcttcc tattactatt ttgacggaga
      901 ttatgttgca gctgataaat attttaaaca atctcttaaa tatgacccta aatcttacac
      961 catcagaaaa caatccctct taaattcttt tgaactttat aataaaaaag gtatatcagc
     1021 aaaaaagctc cagaaattga ttgatgatta caaaaagttt gctcccttag atgaagattt
     1081 gctttatgct gcttatgact tttatagata cactcaaaat gaaacggaac ttataaaaat
     1141 tatttcagag ttacaaacta aatatccttc tccacgtgcc tatcttcaga gtttcctttt
     1201 tgaattgcag tatcatcaaa gagtttatcc agaatatcta tctaaagctc aagaactggc
     1261 tggaaatgac cctacttttt atccactatt gattagactc tggtcatttt ttagtcctga
     1321 aatggaaaaa caggcaatta ttcgttatta tgagctggat aaaggagagc aatccaatgc
     1381 ttttctggca gaatatatag ttcgtaatca ggacttggag cttgctcaac agtatctttc
     1441 ttccctccat tatcctgaag ataaggatta tatgctctat tttgccactg ctcaatggtc
     1501 taatcaacct aatccattac ttcctgattt agctgattat tttctccgga cctcagattt
     1561 agaactcatt tcagaacttg ctctttctgc tctaactcaa catcgctggg acttgtttcc
     1621 tgaaattgaa ctaacagttg aagcttcttc tgcatctgat gaagaaaaac aatctttgta
     1681 ttctctctta attgcttatt ctttatatac ttccaatgat aaaaacttag ctgatattct
     1741 ggaaaaacta accgaattaa aatactttga tgctgtcttt atttactata gctgtgcagt
     1801 taatcaaaaa atagacccca atgttaaact tccaacagct gaagattatg aactcttcat
     1861 taatgaaatt aaaaaacgct tacctgatga acctccagct cgttatcttc aaaccattgc
     1921 ttcaccagag gaagagaatt ccgaaccttt aattttagat agtaaatatc tactcgctct
     1981 gtggcttaaa gaacatcata cactttgtcc tgaagattat gaatttatca tttcttattg
     2041 ccacttaaaa aagaatttct ccgaagaacg aaactttctt ttagaagcta ttaacaaatg
     2101 gcccgactat ccttctttct ataacgacct gggatattct atgctgataa atggagaaga
     2161 cccagaacag gctgcaacct tgattcgcaa agccctcact tatgaacctg aaaaccctta
     2221 ttttctggat agtcttgcct ggtataatta tctcacagga aattactccg aagctttaaa
     2281 tcttatgtct atcccgcaaa aaatggaaaa tatgcccgca gaaatagcct atcatcttgg
     2341 agcaatccat cttgctttgc ataattataa agatgcccac ctctggttac aaaaatgcct
     2401 tactataaat aacgaccctg aagctgaaga actggcaata aaagccctaa aacagatacc
     2461 aaaataatta tacctatatc tcaaaaaacc cgaaacaaat atccacttaa ttacacacat
     2521 atctgcagaa cttaatagta atcattattt cccttgccaa aactataatc tttattaatt
     2581 ttctctttac tccaaaaaat agcattattc tattgacaaa tatcagcccc ttttaaatta
     2641 gttcttgaaa tgcgttccgg aatagctcaa tggtagagca ggtggctgtt aaccacttgg
     2701 ttgggggttc gaatccctct tccggagcca ttt
//
LOCUS       DAORZF010000077         2720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_24953_length_2720_cov_374.909193, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000077 DAORZF010000000
VERSION     DAORZF010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24953_length_2720_cov_374.909193"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="PK015_06900"
     CDS             complement(<1..416)
                     /locus_tag="PK015_06900"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOL34216.1"
                     /translation="MDVIPYKYCPLNCVYCEVQRTNHLTVRRQEFFPPEEIISEVREV
                     LSTNPKLDYVTFSGAGEPTLYSRIGDIILRIKEEFPQYKLALLTNGVLLKHPTVRKEI
                     LPLDLILPSLDACHQDTFEKINRPHPSVKVSDLISGL"
     gene            complement(476..2704)
                     /gene="bamA"
                     /locus_tag="PK015_06905"
     CDS             complement(476..2704)
                     /gene="bamA"
                     /locus_tag="PK015_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOL34217.1"
                     /translation="MPCLILICCFSSLFAAGETIYEIRVEGNRNVPEQLILSSFLLRT
                     GDPLDPEKVAQSIQQIYKMDMFSDIRVYSEPYKSGVNLIIQVAENPVLAHLDYEGIKA
                     IKKDRLDELIQTRSGTFWNEYKKHDLLSKLKAEYESKGFGNASIEILEEPTEDGKVKV
                     KIIVDEGKRFSIKEIVFVGNEHFDDKTLLKRMKTKPASLLRSGRFVEDKFEADLKSLS
                     DFYKKNGFIDVKVGPYELRKIGDKYLELVLNIQEGAQYLLSGITVTGNEHFTTEKILS
                     CFTLKYNEPFDQEKFNAQLANVYSLYFDEGFIYTYIDQEIQKEGNQLNIVLKITEGNR
                     ARIRQINITGNRRTKDKVIIRALDIAPGDYFRQSQVIRSQQNIYNLGFFEPNIKLDYT
                     PINSNGDIDLEINVTDKPSGVANGGVGYNSADGFIGQLSVSMGNIMGNNWSSSLSWEF
                     GRYTEDFQFSFTNPNFLDTDLLLGTSVYYTTKDWSSFYYKIYTRGASIRVGEYLPFID
                     RTRLLAGYSIYSKKYRITNMNMIMQDPENNATLIELDSLDWRYTSSVNATISRDSRDN
                     IFFPTKGSQLTLFSEIAGGILGGDFDYFKQIAQVNWYMELWYKFVLRTKWRFGYITPY
                     GRSNDAPPDEKFYLGGTGVDGIRGYPDRSIGPNGGGSREIIFSTELGFPIAGDQIIAL
                     AFFDAGDSYNKLRTFNFMNMKKGIGLGIRIQSPFGLIGFDYAYNLDEDHWEPHLQIGT
                     TF"
BASE COUNT          812 a          589 c          474 g          845 t
ORIGIN      
        1 agaccagaaa ttaaatctga aaccttaacg gaaggatgag gacgatttat cttctcaaaa
       61 gtatcctgat gacaggcatc taaagaagga agaataaggt ccagaggaag gatttctttg
      121 cgaacagtag gatgttttaa aagaactcca ttggtcaata atgccaactt atattgaggg
      181 aattcttctt ttatcctgag aataatatct ccaattctgc tataaagagt tggttcacca
      241 gcaccagaaa aagtgacata atccagtttt ggattagttg ataaaacttc tctcacttcg
      301 gaaatgattt cttctggagg gaaaaactcc tgtctccgca cagttaaatg attggtgcgt
      361 tggacttcgc aataaacaca attcaaggga caatatttgt aagggataac atccacccct
      421 agggatatcc ctaatcttcg tgatgggact ggaccaaaaa tatgtttgta tggcattaaa
      481 aggtggtccc tatctgtaaa tgtggttccc agtggtcttc atcaagatta taagcataat
      541 caaatccaat taaaccaaag ggactttgga tccgaatgcc caatccgatt ccttttttca
      601 tattcataaa gttgaaagtc cttaatttat tgtagctatc tccagcatca aagaatgcca
      661 gagctattat ctggtcaccc gctataggaa atcccagttc ggtggaaaag ataatctctc
      721 ttgaccctcc accatttggt cctatggaac ggtctggata acctctaatg ccatctactc
      781 ccgttccacc aagatagaac ttttcatcag gaggagcatc atttgacctt ccataaggag
      841 ttatataccc aaaacgccat tttgttctaa gaacaaattt ataccacaat tccatatacc
      901 agttcacctg ggcaatctgc ttgaaataat caaaatctcc acctaaaatt ccgcctgcta
      961 tttccgaaaa gagtgtcaat tggcttcctt tagtaggaaa gaatatatta tcccggctat
     1021 cccgacttat ggtggcatta actgaactgg tgtatctcca atccagagaa tccagttcaa
     1081 ttaaagtggc attgttctca ggatcctgca taatcatatt catattggtt atgcgatatt
     1141 ttttggagta gatggaatag ccagccagta gacgggtgcg atcaataaaa ggaagatact
     1201 ctccaacccg aatgcttgca ccacgagtat agattttata atagaaagaa ctccagtcct
     1261 tagtagtata ataaacagaa gttcctagaa gaagatcagt atccaaaaaa ttaggattgg
     1321 tgaagctaaa ttgaaaatct tcagtatagc gtccaaattc ccagcttaaa ctgctggacc
     1381 aattattgcc cataatgtta cccatactaa ctgaaagctg cccaatgaag ccatctgcac
     1441 tgttgtagcc tacgccacca ttggcaacgc cagagggttt atcggtaaca ttgatttcca
     1501 ggtctatatc tccattgcta ttgataggag tgtaatccaa ctttatgttt ggctcaaaga
     1561 agccaagatt ataaatattc tgctgactgc gtataacttg actctggcgg aaataatctc
     1621 ctggtgctat atctaaagcc ctgatgatta ccttgtcttt ggttctgcga ttacctgtta
     1681 tattaatctg ccttatcctg gctctattac cttccgttat cttcagaact atattgagct
     1741 gatttccttc tttctgaatc tcctggtcta tataagtata gataaaacct tcgtcaaagt
     1801 atagcgaata gacatttgcc agctgagcat tgaacttttc ctggtcaaag ggttcattat
     1861 atttcaaagt gaaacagctc aaaatttttt ctgtagtaaa gtgctcattt cccgtgaccg
     1921 ttataccaga aagtaaatat tgtgcacctt cctgaatatt tagcaccagc tccagatatt
     1981 tatctcctat cttacgaagt tcgtagggtc caacttttac atcaataaaa ccattcttct
     2041 tgtagaaatc ggaaagactt ttcaggtctg cctcaaattt atcctctaca aaacgacctg
     2101 aacgcagtaa acttgctggt ttggtcttca ttcttttcag taaggtcttg tcatcaaagt
     2161 gctcatttcc tacaaagacg atttctttaa tggaaaagcg cttaccctca tcaacgatga
     2221 tttttacttt cactttgcca tcttcagtcg gctcttctaa aatctctatg ctggcattac
     2281 caaatccctt gctttcatat tccgctttga gcttgcttaa taaatcgtgc ttcttgtatt
     2341 cgttccagaa agtgccactg cgagtttgaa tcagctcatc caatctatct ttcttaatcg
     2401 cttttatacc ttcataatcc agatgtgcca aaactggatt ctctgctact tgtataatga
     2461 gattcactcc tgatttatag ggctcactgt atacccttat atctgagaac atatccattt
     2521 tgtatatctg ctgaatggat tgagctacct tctccgggtc taaaggatcc cctgtcctca
     2581 ataaaaaaga agataggatc aactgttccg gcacattccg gtttccttcc actcggattt
     2641 catatatagt ctcacctgca gcaaataaac tgctgaagca acaaatcagt atcagacaag
     2701 gcaatagcca tctttgccag
//
LOCUS       DAORZF010000078         2684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_25436_length_2684_cov_356.282997, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000078 DAORZF010000000
VERSION     DAORZF010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25436_length_2684_cov_356.282997"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1292)
                     /locus_tag="PK015_06910"
     CDS             complement(<1..1292)
                     /locus_tag="PK015_06910"
                     /inference="COORDINATES: protein motif:HMM:NF012975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HOL34218.1"
                     /translation="MFNLASGKIVLFFQDGNLQSGIITEAQNRHYKVLNQEAKTYILP
                     PARFILVTQKVYEPASTKTLEDFEKQIKNKIEEEGENWLLQLQETQDSFTFEEICSKF
                     GFEGDIERFALFILLQQRKDLFKFKKDVFSFRNEEERLKYLQEEAETRERTRYLKEVE
                     KFLQTYQDLLPSLNILFPIKDDVSQGFDLESKTQFLSELRELLRSNAPKDLAKLLHKY
                     SDGLDLRTLIRNIRLLLRDISPDTDKIAADSGIPINFSSEILREAQKLQEKLKQEAEN
                     QNVTNLEAFTIDAIDSPDYDDAISLQKQEQGWILGIHISDVGGQLPLNSSIFKEACER
                     VSSLYLPCETIPLLPPSLSKEAFSLKAGEKRKVLSLYLFMDKEFKIEKEVFKQEYITV
                     KQNLSYEEVDRYLTSHQNSTLLQIVHHIHNGRQIFESSG"
     gene            complement(1310..1385)
                     /locus_tag="PK015_06915"
     tRNA            complement(1310..1385)
                     /locus_tag="PK015_06915"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1350..1352),aa:Arg,seq:cct)
     gene            1496..2476
                     /locus_tag="PK015_06920"
     CDS             1496..2476
                     /locus_tag="PK015_06920"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF011456.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOL34219.1"
                     /translation="MKIAIVGATGEVGRMMLTCLDEFNVPMNTIDLFASERSAGKVLY
                     LGDRALTVKKLEEDSLSRSYDYVLFSAGAGVSLHFAPIAAQNSRAVIDNSSAFRKEKD
                     IPLVVPEINGHLIEHYHGIISNPNCATIQMVLPLAILDHLYGLQKIVVSTYQSVSGSG
                     HKGIETLLAQRKGSQDLGVYPELIDLNVIPQIGAFLDNGYSQEEEKLIFESQKILNLP
                     NLKVCPTAVRVPVLYGHSESIYAEFDRTINLEEAAEALKKAESIAYEEHTYMTPRGLN
                     SSNDSFVCRLRYGVDNHSLCFWNVANNVRLGAATNAVKILIRHAKSEGLL"
     gene            2473..>2684
                     /locus_tag="PK015_06925"
     CDS             2473..>2684
                     /locus_tag="PK015_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34220.1"
                     /translation="MSLDLISIRRELHQVPELAFHEFETKAILLKYLKTLPDINIIEF
                     LNSPGILVEYSQGNGLYRLFRADMDAL"
BASE COUNT          773 a          525 c          498 g          888 t
ORIGIN      
        1 ccgctacttt caaatatctg gcgtccattg tgtatatgat gcactatctg aagtaaagta
       61 gaattctggt gactggttaa atatctgtcc acttcttcat agctgagatt ttgtttcacc
      121 gtgatatatt cttgtttaaa tacctccttt tcaatcttga actccttatc cataaataga
      181 tataaggaca gaacttttct tttttctcct gcttttagac tgaaagcctc tttagaaagt
      241 gaaggtggaa gcaaaggaat agtttcacaa ggaagataca gagaactaac tctttcacag
      301 gcttctttaa aaatggaaga atttagcgga agttgacctc ctacatcact gatatgaata
      361 cccagaatcc agccctgctc ctgcttctga agcgagatgg catcatcata atcaggagaa
      421 tctatcgcat caatagtaaa agcctccaga tttgtcacat tctgattttc tgcttcctgc
      481 tttaatttct cttgcaattt ttgtgcttct cgcaggattt cagaactaaa atttatcgga
      541 ataccgctat cagcagctat cttatccgta tctggagaaa tatccctcag aagcaaacga
      601 atattcctga ttaatgttct caaatccaga ccatcgctat atttatgaag aagttttgcc
      661 aggtctttgg gtgcatttga acgaagcaat tctcttaatt cagatagaaa ttgggtcttg
      721 gattccaggt caaatccctg agaaacatca tcttttatag ggaacaaaat atttagggaa
      781 ggtaataaat cctgataggt ctgaagaaac ttttctactt cttttaggta ccttgttctt
      841 tctctggttt ctgcttcttc ctggaggtat tttaatcgtt cttcttcatt gcggaaggaa
      901 aacacatctt ttttgaattt gaacaagtct tttctttgtt gaagcaagat aaacaaggca
      961 aaacgttcta tatccccttc aaatccaaat ttggagcata tctcttcaaa agtgaaagaa
     1021 tcctgagtct cttgcaattg tagaagccag ttttcacctt cttcttcaat tttattcttg
     1081 atttgcttct caaaatcttc caaggtcttc gtagatgcag gttcatacac tttttgagtg
     1141 accagaataa atcgtgctgg aggcaaaata taggttttag cctcctgatt aagcacttta
     1201 taatgtctat tttgagcctc tgtgattatt ccagactgga gattaccatc ctgaaagaag
     1261 agaactattt tgccgcttgc aaggttaaac atataatcct ttatttttat ggcgccccag
     1321 agatgatttg aacatccgac caacggttta ggaaaccgct gctctatcct ctgagctact
     1381 ggggcgtctt ttcttttcct atcctttgaa aatcaacttt ttgtcaagtt ttgcattgac
     1441 aaaagcatct aatattagga aaaggatttt tctgataata aataaggaat taccaatgaa
     1501 aatcgctatt gttggtgcta caggtgaagt gggtagaatg atgctcacct gtctggatga
     1561 attcaatgtg ccaatgaaca ctatagacct ctttgcttct gaacgctctg ccggaaaagt
     1621 gctttatcta ggcgaccgtg ctttaaccgt aaaaaaattg gaagaagatt cactctcccg
     1681 ttcctatgac tatgttttat tctctgctgg tgctggtgtt tccctacatt ttgctcccat
     1741 tgctgctcaa aattcaagag cagtcataga taattcttcc gctttccgta aagaaaaaga
     1801 tatacctctt gtcgttccag aaataaatgg acatctcata gaacattatc acggaattat
     1861 ttctaatcca aattgtgcta cgattcagat ggtgctccct ctggcaattt tagaccatct
     1921 ttatggttta caaaaaatag tagtcagcac ctatcaatct gtttcgggca gtggacacaa
     1981 aggtattgaa accttgctgg cacagcgtaa gggaagtcaa gacctgggag tttatccaga
     2041 gcttatagac ctcaatgtta ttcctcagat tggagctttt ctggataatg gatattctca
     2101 ggaagaagaa aagctaatct ttgaatcaca aaaaattctc aatctgccaa atttgaaggt
     2161 ctgccctact gctgttcggg ttcctgtgct ttatggtcac agtgaaagta tctatgccga
     2221 atttgataga actatcaatc ttgaagaagc tgcggaagct ttaaagaagg ctgaatctat
     2281 cgcttatgaa gaacatacct atatgactcc ccggggctta aattcctcta atgatagttt
     2341 tgtgtgtcgt ctgcgttatg gagtggataa tcattctctc tgtttctgga atgtggctaa
     2401 taatgttcgt ttgggcgcag ctactaatgc cgtaaaaata ttaattcgtc atgccaaaag
     2461 tgaaggttta ttatgagctt ggatttaatc agtatccgta gagagttaca tcaagtacca
     2521 gaacttgctt ttcacgagtt tgagaccaaa gctatcctgt taaaatatct caaaactcta
     2581 cctgatatta atatcataga attcctcaat agtcctggaa ttttggtaga atacagtcaa
     2641 ggtaacggac tttatcgtct tttccgtgct gatatggatg cact
//
LOCUS       DAORZF010000079         2651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_25854_length_2651_cov_279.726888, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000079 DAORZF010000000
VERSION     DAORZF010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25854_length_2651_cov_279.726888"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1925
                     /gene="ppdK"
                     /locus_tag="PK015_06930"
     CDS             <1..1925
                     /gene="ppdK"
                     /locus_tag="PK015_06930"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOL34221.1"
                     /translation="AFTRDAATGEHIFNGEYLINAQGEDVVAGIRTPLQITKIGSQRW
                     AASLGISEEERLAKYPSLEEAMPEVYKQLFEIQKKLEEHAKDMQDLEFTIQEGRLWLL
                     QTRTGKRTGFAMVKIAMDMVREGLIDEKTALLRMDPNKLDELLHPVFSQEGLAKAKEI
                     AKGLPASPGAATGQIVFFAEDAEVWAAQGKKVILCRLETSPEDLRGMAVAQGILTARG
                     GMTSHAAVVARGMGKCCVAGVGTIVIDYKNRTMTAKGKTYHEGDWISLNGSTGQVFEG
                     KIETVAPELSGDFAELMDLADKYTRMYVRTNADTPQDASVARKFGARGIGLCRTEHMF
                     FTGNRIKAMREMILADNEEGRRKALDKLLPMQRSDFEGIFTAMDGFPVTIRLLDPPLH
                     EFLPHEENQQKEIAEELGISFQKVKERVNALHELNPMLGHRGCRLGNTYPEITEMQTR
                     AIIEAALNVKAKGIKVIPEIMVPLVGIETEFKMQDEIIRSTAEKIFAERGDRIDFLVG
                     TMIEVPRAALIADKIAESAEFFSFGTNDLTQMTFGYSRDDAGVFLPIYLDKHILKNDP
                     FQILDQEGVGQLVQMGTERGRKTRPNLKVGICGEHGGEPSSVEFCHRVGMDYVSCSPY
                     RVPIARVAAAMAAIRE"
     gene            2288..>2651
                     /locus_tag="PK015_06935"
     CDS             2288..>2651
                     /locus_tag="PK015_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HOL34222.1"
                     /translation="MDNNKKSPNEEQGYAEEQGKIDAKKLKFYENLRQKAREWTKEKT
                     GTAGNKLAEYLFLLPDFFILVCRIAVDKRVPAHIKLKLAGIIAYVMMPLDLIPDFIPI
                     MGSIDDLVLIVLGLNMILN"
BASE COUNT          822 a          461 c          589 g          779 t
ORIGIN      
        1 tggctttcac tcgtgatgct gctaccggtg agcatatttt caacggagaa tatctgatta
       61 atgcacaagg tgaggatgta gtggcaggaa ttcgtactcc tttgcagatt actaagattg
      121 gctctcagcg ttgggctgcc tctttaggaa tctctgaaga agaacgcctt gctaaatatc
      181 cttccctgga agaagcaatg ccggaagttt ataaacaact ctttgaaatc cagaaaaaat
      241 tggaagaaca tgctaaagat atgcaggatt tggaattcac tatccaagaa ggaagactgt
      301 ggctgctaca aactcgtaca ggaaaacgca ctgggtttgc tatggtaaaa atagcaatgg
      361 atatggttcg ggaaggctta attgatgaaa aaactgcact attgagaatg gaccctaaca
      421 agctggacga gcttttacat cctgttttct ctcaagaagg cttggctaaa gcaaaagaaa
      481 tagctaaggg acttcctgct tctcctggag ctgccaccgg tcagattgta ttctttgcag
      541 aagatgctga agtctgggct gcacaaggta aaaaggtaat tctatgtcgt ttagagacct
      601 ctcccgaaga tttgcggggg atggctgttg ctcaaggcat tttaaccgct cgcggaggta
      661 tgacttccca tgctgctgtg gtagcaagag gaatgggtaa atgctgcgtc gccggggttg
      721 gaactattgt gattgattac aaaaaccgca ctatgactgc taaaggtaaa acttatcacg
      781 aaggtgactg gatttctctg aatggaagca ctggtcaagt ctttgaaggg aaaattgaga
      841 ccgtagcacc tgaactgagt ggagattttg ctgaacttat ggatttggca gataaatata
      901 ctcgtatgta tgtgagaacc aatgcggaca ctcctcaaga tgcctcagtt gctcgtaaat
      961 ttggagctcg gggtattgga ctttgcagaa ctgagcatat gttctttacc ggaaatcgca
     1021 ttaaagctat gcgtgagatg attttggctg ataatgaaga aggtagaaga aaagctctgg
     1081 ataagctatt acctatgcaa cgctccgatt ttgaaggaat ctttaccgct atggatggtt
     1141 tccctgtcac cattcgttta ttagaccctc ccttgcatga attcctccct catgaagaaa
     1201 atcaacaaaa agagatagca gaggaactgg gaatttcttt tcaaaaagtt aaagaaagag
     1261 tcaatgcttt gcacgaactc aatcctatgt tgggacatag aggctgccgt ttaggcaata
     1321 cttatcccga aattacggaa atgcagaccc gtgccataat tgaagcagct ctcaatgtta
     1381 aagctaaagg aattaaagtg atacccgaaa ttatggtacc cttagtagga attgaaactg
     1441 aattcaagat gcaggacgag attatacgta gcacagctga aaagattttt gccgaaagag
     1501 gtgatagaat tgactttttg gtgggcacaa tgattgaagt accgcgtgct gctttaattg
     1561 ctgataaaat tgcagaaagt gcagaattct tcagttttgg aacgaatgat ttaactcaaa
     1621 tgacctttgg ttattctcgt gatgacgcag gtgtcttcct tcctatctat ctggataaac
     1681 atatacttaa gaatgatcct ttccagattt tagaccagga aggagtggga caattagttc
     1741 agatgggaac tgagcgcggt agaaaaaccc gacctaatct gaaagtgggt atctgtggag
     1801 agcatggtgg tgaacccagc tccgtggagt tctgtcatag agtcggtatg gattatgtta
     1861 gctgttctcc ttatcgcgtt cctattgcaa gagttgccgc agctatggct gcaattagag
     1921 aataaatgat taattgaaca ccggagctta aaaactccgg tgttttttat ttttctctct
     1981 ttcccaattt taaaattttt tctcttagtt caaaattctc tcttggctct cccttagctc
     2041 ttttgtaagt aaattgataa gagagagtca agtaggagat tgattagagc taacctgctg
     2101 tcatccatta tattagaccc aaaatagtcc tatttataag ccattataaa gaaaattatt
     2161 ttatctattt tttaataaaa tctcggtttc tttaacgcta aaatagcttg aatgtttaaa
     2221 atagattgac taaataaatg ttttagatta taatagatta taagagaaac tttaaaaaga
     2281 ggattcaatg gataataaca agaaatctcc taatgaagaa cagggttatg cagaagagca
     2341 aggtaaaatt gatgccaaaa aactaaaatt ttatgagaac ttacggcaaa aagccagaga
     2401 atggactaaa gaaaagaccg gaactgcagg caataaatta gcggaatatc tatttttact
     2461 gcccgatttc tttattctgg tctgtcgtat agctgtagat aaaagggttc ctgcacacat
     2521 aaagttaaaa ttagccggaa tcattgctta tgtaatgatg cccttagact taattccaga
     2581 tttcatccct attatgggtt ctattgatga tttagtgctt atcgtactgg gtttgaatat
     2641 gatacttaat g
//
LOCUS       DAORZF010000080         2315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_31082_length_2315_cov_333.886283, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000080 DAORZF010000000
VERSION     DAORZF010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31082_length_2315_cov_333.886283"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..2076
                     /locus_tag="PK015_06940"
     CDS             157..2076
                     /locus_tag="PK015_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOL34223.1"
                     /translation="MSLIRAIDVSVEFSGKSILRNINCSLEHNSKIGLIGANGSGKST
                     LLKVLYGMQTPTSGKVQKAKYCNIAYLPQDYHPLSNRVLIEYIRSARPELLRLEREIE
                     RLSEELNKNPDEEIEAELNEVIEQYTYYGGYDFENEIKYVLTSLNIPPESWNRKCGEF
                     SGGEQTRIALANVLLTNGDLIMMDEPTNHLDLEMITWLEKYLQNLKKPFIVVSHDRQF
                     LDNTVKTIWCLRDGQLTITKGNYSTFRKADEIAQLSQARQYEQQKKYIEETENFIRQN
                     IAGNRSNLAKSRLKQLERMEIVKKPSDQKSIKLSIEANKRSGNDIYILENVSYGISPD
                     KELARDINLTVNYKDRICILGPNGCGKTSLLKVLLQDSDIFSGTLKVGASLDIGYFDQ
                     QQNDLDPELTVLDTIWQIIPEATIGYVRSWLARFGFYDDDIDKKVEQLSGGEQSRLYL
                     CRLIHTQPNLLLLDEPTNHLDLQMTESLMEALKNYNGTIIFVTHDRWFMKELTTKFWV
                     FRKLKAENGFYTTIQEYIGNWEEAMNLAFEKPEATKTPPPPRKRKNRINPWHMRSILG
                     EIEESSREYELLIEEMQEIQYELSQSTTYSSPEKVKLLQKQLKELEETMTQLKELIDD
                     MEEKYLELYYQYYDK"
     gene            2066..>2315
                     /locus_tag="PK015_06945"
     CDS             2066..>2315
                     /locus_tag="PK015_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HOL34224.1"
                     /translation="MINKRIGFTSSFPVEVVYAAGYTPIDLNNIFVEGNSTEYIRKAE
                     LKGFPRTICAWIKGNFAAALSANLDEVIGISQGDCSNGI"
BASE COUNT          816 a          394 c          449 g          656 t
ORIGIN      
        1 accactaatg aaggtataac ttcccaaatc aaatcgggat ttaaatataa attgacaatg
       61 agagaatagc tggaaaatgg ttaaataaca ttatgataaa taaaataaat agattatata
      121 atcatatttt aataatttta aagccgggga acaattatga gtcttattcg tgctatagat
      181 gtatctgtag aattcagtgg taaatctatt cttaggaaca ttaattgcag cctggagcat
      241 aatagcaaga taggtttgat tggagcgaat ggttccggta aaagcacatt actcaaagtg
      301 ttatatggaa tgcaaacgcc cacttctgga aaggtgcaaa aagctaaata ttgtaatatt
      361 gcataccttc ctcaggacta tcatccctta tccaatcggg ttcttataga atatattcgc
      421 tctgcccgtc ccgaactttt gagattggaa agagaaatag aaagattatc cgaggaatta
      481 aataagaatc ctgacgaaga aattgaagca gaattaaatg aggtaataga acaatatacc
      541 tattatggag gttatgattt tgagaatgag attaaatatg tgctcacttc acttaatata
      601 cctccggaaa gctggaaccg aaaatgcgga gaatttagtg gaggagaaca aaccagaatt
      661 gctttagcaa atgttttatt gactaacggt gacctgatta tgatggatga gccaaccaat
      721 catcttgatt tagaaatgat aacctggctg gagaaatatc tacaaaatct gaaaaaacct
      781 tttattgttg tctctcacga ccgtcagttt ctggataata cggttaaaac tatatggtgc
      841 ttgcgtgatg gtcaattgac tattaccaag ggaaactata gtacttttcg taaggcagat
      901 gagattgctc aactttctca agcacgacaa tatgagcagc aaaagaaata tattgaagag
      961 acagagaatt tcatccggca aaacattgcg ggaaatcgta gcaatttagc taaatcccgt
     1021 ttaaagcaat tagaacgaat ggaaatagta aaaaaaccgt ctgaccagaa atctataaag
     1081 ctctccatag aagccaataa gcgcagcggt aatgatatct atattttgga aaatgtctct
     1141 tatggcattt ctcctgataa agaactggca agagatatca atctaaccgt caattataag
     1201 gatagaatct gcattttagg tcctaatggt tgcggaaaaa cttctttatt aaaggtcctt
     1261 ttacaggata gcgacatctt tagtggaact cttaaagtag gagcaagttt ggatatagga
     1321 tattttgacc agcaacagaa tgacttagac cctgagctaa ccgtgcttga tactatctgg
     1381 caaattattc ctgaagcaac tataggttat gtgagaagct ggttagctcg ttttggattt
     1441 tacgacgatg atattgataa aaaggtggag caattaagtg gaggtgaaca atctcggctt
     1501 tatctttgcc gattaattca cactcaacct aacctattac ttttggatga acctaccaat
     1561 catcttgacc tgcagatgac tgaatcgtta atggaggctt taaaaaacta taatggaact
     1621 attatctttg taactcacga ccgctggttt atgaaagaac tgactaccaa attctgggta
     1681 tttcgcaagc tcaaagctga aaatggtttc tacacaacca ttcaagaata cattggcaac
     1741 tgggaagaag cgatgaatct ggcatttgaa aaaccagaag caactaagac tcctccccca
     1801 cctcgtaaac gaaaaaaccg catcaatccc tggcatatgc gcagcattct tggcgaaatt
     1861 gaagaaagta gcagagaata tgaactgtta atagaagaga tgcaggaaat tcaatacgaa
     1921 ctatctcaat ctactaccta ttcctcacca gaaaaggtaa aattattaca aaaacaactg
     1981 aaagaactgg aagaaacaat gacccagttg aaagaactga ttgatgatat ggaagaaaaa
     2041 tatttagaat tatactatca atattatgat aaataaaagg attggcttca cttcttcctt
     2101 ccccgtagaa gttgtctatg cggcaggtta tacacccatt gacttaaata atatctttgt
     2161 ggaaggaaat tctaccgaat atattcgtaa agctgaattg aaaggatttc cccgcaccat
     2221 ctgcgcttgg attaaaggca attttgctgc tgcactttcc gctaatctgg atgaagtgat
     2281 aggaatttcc cagggagatt gttctaatgg cattt
//
LOCUS       DAORZF010000081         2149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_34402_length_2149_cov_249.735435, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000081 DAORZF010000000
VERSION     DAORZF010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34402_length_2149_cov_249.735435"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="PK015_06950"
     CDS             complement(<1..808)
                     /locus_tag="PK015_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HOL34225.1"
                     /translation="MALPKVSPHMGEYASRLLRSLEYRGYDSTGAAFQGEDENIILLK
                     DVGAPSTLVKTLGIEKQSGKLFCGQVRWATFGAVDKNNAQPHQVKCKRHLYGAHNGNI
                     TNTPELKVWLTQEGHIVVSDNDGEMLVHTVEHFFDIEMDRAGNPTKKEAIKICMRQAL
                     INTGEKLIGSYAAVITEPITQTMWAMKAGSSLYFGLGQLEGMPFALASSDLTAVLRFT
                     KTLVNLREGEFIEFNSDYYQVYAQKNLKFKRVNAPDEVYLAGDKIPKQPVL"
     gene            complement(934..1635)
                     /gene="ispD"
                     /locus_tag="PK015_06955"
     CDS             complement(934..1635)
                     /gene="ispD"
                     /locus_tag="PK015_06955"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOL34226.1"
                     /translation="MEKILNTAIVCAAGSGIRMGSSVKKQWLPLKGIPIIIHALKKFF
                     SSSIIHNIIIPAPEEDLDFCQELIAKYFHDSDKPWLVMPGGVERQDSIFAALQHCPGD
                     TNMVFIHDAVRPFITEELLDKLYDIALKEKAVVPVTRIKNTVKKIAKDYIVETVPREN
                     LVQALTPQVFSYELIMSAYFKAYEDGYISTDDSALVEYYGAKVRYQFCSELNLKITDE
                     LDLFFAEKIIENKLI"
     gene            complement(1620..>2149)
                     /locus_tag="PK015_06960"
     CDS             complement(1620..>2149)
                     /locus_tag="PK015_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOL34227.1"
                     /translation="KINRPHPSVKVSDLISGLIDLRKEYSGFIWLEVFIIAGLNDSEE
                     ELECLSKALNRIQPDKVQLNSLDRPGAEFWVKPAGSRVLREVQRFLSERVNMPVEIIA
                     RIPSDKDELIIPPEIEQKFATLLNDHPSTPEEISKASGLHINEVSHYLRQLHLEHKIS
                     IKQGQKGPLFVWKKS"
BASE COUNT          619 a          438 c          376 g          716 t
ORIGIN      
        1 aaaggacagg ttgtttagga atcttatctc ctgccaggta tacctcgtct ggtgcattca
       61 ctcttttgaa tttaaggttt ttttgtgcat agacctgata gtaatcagaa ttgaattcta
      121 taaattcgcc ttcgcgaaga ttgactaagg ttttagtgaa acgtaagact gcagtaagat
      181 cggatgaagc cagggcaaaa ggcattcctt ccagctgtcc taaaccaaaa tataggctgc
      241 tgcctgcttt catcgcccac attgtttgtg taattggctc agttataact gcagcatagg
      301 aaccaatcag cttttcacct gtattgataa gggcttgacg catgcagatt tttatagctt
      361 cttttttcgt tggattgcct gctctatcca tttcaatatc aaaaaaatgt tccactgtat
      421 gaacaagcat ctctccatcg ttatcggaaa caactatatg tccttcttga gtaagccaga
      481 ctttaagctc tggtgtatta gttatatttc cattatgcgc accatataga tggcgtttac
      541 attttacttg atgcggctga gcgttatttt tatctactgc tccaaaagtt gcccaacgca
      601 cctgtccaca gaaaagctta ccactttgtt tttctattcc cagagttttt actaaagtgc
      661 tcggtgcacc tacatcttta agtaaaatga tattttcatc ttcaccttga aatgctgctc
      721 cagttgaatc ataacctcta tattctagag aacgaagtaa cctggaagca tactctccca
      781 tatgaggtga aacttttggc aatgccaacc ctagaacacc gcatcctatc cctggtaaaa
      841 ataaagggat atgaatattt tctattattc gggctaggta ttctaatatt tgattgtata
      901 ttctcaataa tttttgcata gaaaaattcc ttattaaatc agcttgtttt ctattatctt
      961 ttctgcgaaa aataggtcaa gctcatcggt gatttttaaa tttaattcag aacagaactg
     1021 atatcttacc tttgctccat aatattctac taaggctgaa tcatctgtgc tgatataacc
     1081 atcctcatag gctttgaaat aggcagacat tatgagttca taggaaaaaa cttgaggagt
     1141 taatgcctga accagattct ctctggggac agtttctact atataatctt ttgcaatttt
     1201 cttaaccgta ttcttaatcc ttgttacagg tactaccgct ttttctttta gagctatatc
     1261 ataaagcttg tccagaagtt cttctgttat gaagggacgc accgcatcat ggataaaaac
     1321 catatttgtg tcacctggac aatgctgtaa agcagcaaaa atgctatcct gcctttccac
     1381 accacctggc ataactaacc agggcttatc tgaatcgtga aagtatttag caataagttc
     1441 ctgacagaaa tccagatcct cttcaggagc aggaatgatg atattgtgaa taatggatga
     1501 gctgaaaaac ttctttaatg catggataat gataggaatt ccctttaatg gaagccattg
     1561 ttttttaact gagctaccca ttctgattcc agaacctgct gcacatacta ttgccgtatt
     1621 caggattttt tccatacaaa taagggccct ttttgcccct gttttatact aattttatgt
     1681 tctaaatgca attgacggag ataatgacta acttcattga tatgaagacc tgaagcttta
     1741 gatatttcct ctggggtaga agggtggtca ttaagaagtg tagcgaactt ttgttctatc
     1801 tcaggaggaa ttatcaactc atctttatca gaaggtattc tggctattat ttccactggc
     1861 atattaactc gttcggacag gaatctttgc acttctcgca gaactctgct tcctgcaggt
     1921 ttaacccaga actctgctcc tggtctatcc agagaattca attgcacctt atctggctga
     1981 attcggttta gtgccttact taaacattcc agttcctctt cagaatcatt cagtccggca
     2041 ataataaata cttccagcca gataaaaccg gaatattctt ttcttaaatc tataagacca
     2101 gaaattaaat ctgaaacctt aacggaagga tgaggacgat ttatcttct
//
LOCUS       DAORZF010000082         2085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_35940_length_2085_cov_318.998522, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000082 DAORZF010000000
VERSION     DAORZF010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35940_length_2085_cov_318.998522"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="PK015_06965"
     CDS             <1..783
                     /locus_tag="PK015_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HOL34228.1"
                     /translation="ANLDEVIGISQGDCSNGISLTAMLAEEGLPIYHFSFPQERTYEA
                     LNAEIRKLEEHFEVSRDEVMKVKKRLDDIRTKLITLDEWTWKEGLITGKENHYWLVNS
                     SDFRGNPDKFEEDLTSFMEEASRRNPSIPKHRCAYLGVPPIYRDLYDRIEELGGRVIF
                     NEVQRQFSMPYLCSDIVEQYLVYTYPYGVFARLEDILSQLNLRKIDMVISYTQSFCHL
                     QIDNILLKKHIKIPFLNLEGDRPEELDSRTLLQLESFFEVYG"
     gene            780..1859
                     /gene="mnmA"
                     /locus_tag="PK015_06970"
     CDS             780..1859
                     /gene="mnmA"
                     /locus_tag="PK015_06970"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOL34229.1"
                     /translation="MKKVVVALSGGIDSAVSAFLLKEQGYEVIGATMSFWDDIALASK
                     WCSQGCYGPQEEENIKSARQIASLLNIEHTVINLRKEFKDIVLTYFISSYMNGKTPNP
                     CVICNAHIKFAAFYEGIKRQGLDFDYFATGHYARKNYDNELHRWQLLKGKDTLKDQSY
                     FLCLLNQEQISRALFPLGERTKEEIKQIATDIGLGFLREREESQDFIDSSVYPYIFPA
                     QAFTAGEIIDSTGKVLGYHQGLVNYTIGQRKHLGISGMTEPYYVVGIDAKHNRLIVGP
                     IKELYKDRLIARDINWVSIPDLKEEIRAETKIRLAHNAASGTLLPFSENRVEVQFDEP
                     QLSITPGQFAVFYKGDLLLGGGVIE"
     gene            1859..>2085
                     /locus_tag="PK015_06975"
     CDS             1859..>2085
                     /locus_tag="PK015_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HOL34230.1"
                     /translation="MYYLLKVIISAGLIVLISEVSKRSSFIGAIFASIPLVSFLAILW
                     MYFETKDTGKIAELSIDIFWLVLPSLLFFIL"
BASE COUNT          668 a          342 c          418 g          657 t
ORIGIN      
        1 gctaatctgg atgaagtgat aggaatttcc cagggagatt gttctaatgg catttcttta
       61 accgctatgt tagctgaaga aggacttcct atctatcact tttccttccc tcaagaacga
      121 acctatgaag ctctaaatgc agaaatcaga aaactggaag aacactttga agtctccaga
      181 gatgaggtga tgaaggttaa aaaaagactg gatgacatta gaactaaatt gataactttg
      241 gatgaatgga cctggaaaga aggattgata acaggaaaag aaaatcatta ctggctggtt
      301 aattcttccg attttagggg taatcccgat aagtttgaag aggatttaac ctcttttatg
      361 gaagaggcat ccagacgcaa tccttctata cctaaacatc gctgtgccta tctgggtgtt
      421 cctcctatct atcgtgacct ctatgaccgc attgaagaac ttggtggtag agttatcttt
      481 aatgaggtac aaagacaatt ttctatgccc tacctctgtt ccgatatagt agaacaatat
      541 ttagtctaca cttatcctta tggagttttt gctcgcctgg aagatatact ctcacaattg
      601 aatttaagaa aaatagatat ggtgattagc tacactcaat ctttttgtca cctgcagatt
      661 gataatatcc ttttgaaaaa acacataaag atacccttct tgaacctgga aggagaccgt
      721 ccggaagaac ttgatagcag aactctttta caactggaaa gcttttttga ggtttatgga
      781 tgaaaaaagt agtggtagca cttagtggag gcattgattc tgctgttagt gcttttttat
      841 taaaagaaca aggatatgaa gttattggtg ctacaatgtc cttctgggat gatattgctt
      901 tagcatccaa atggtgttca caaggttgtt acggaccaca agaagaggaa aatataaaat
      961 cagctcgcca gattgcttct ttactgaata tagagcatac agtcataaac ttaagaaaag
     1021 agtttaagga tatagtacta acttatttta tttccagcta tatgaatgga aaaacaccta
     1081 atccctgtgt aatctgcaat gctcatataa agtttgctgc tttctatgaa ggaataaaaa
     1141 gacagggatt ggattttgat tattttgcta cgggacatta tgcaagaaag aactatgata
     1201 atgaactcca tcgctggcag cttcttaaag ggaaagatac tcttaaagac caatcttatt
     1261 tcctctgctt attaaatcag gaacaaataa gtagagctct ttttcctctc ggtgaaagaa
     1321 caaaagaaga gataaaacaa attgctacgg atataggttt gggatttctc cgggaacgag
     1381 aagaaagtca ggactttata gattcctctg tttatcctta tatctttccc gctcaggctt
     1441 ttaccgcagg agaaattata gacagcactg gcaaagtgtt aggatatcat cagggattgg
     1501 taaattacac tataggacaa cggaaacatt taggaatcag cggaatgact gaaccttatt
     1561 atgtggttgg aattgatgct aaacataaca gattgattgt aggacctatt aaggaacttt
     1621 acaaggatag acttattgcc cgtgatataa actgggtttc tatccctgat ttaaaagaag
     1681 aaataagagc agaaacaaag ataagattag ctcataatgc agcttcagga actttgctac
     1741 ctttttcaga aaatagagta gaagtccagt ttgatgaacc tcaactatcc ataactccag
     1801 gacaatttgc cgttttttat aaaggtgatt tgcttcttgg aggaggagtg attgaataat
     1861 gtattattta ttaaaagtta ttatatctgc tggtttgatt gttctgataa gtgaagtttc
     1921 taaaagaagt agttttatag gagcgatttt tgcttctatt ccattggtct cctttctcgc
     1981 tatactctgg atgtattttg agacgaaaga tacgggaaag attgcagaac tctccattga
     2041 tattttctgg ttggttctgc cttctttact attcttcatt ctatt
//
LOCUS       DAORZF010000083         1987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_38481_length_1987_cov_376.943064, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000083 DAORZF010000000
VERSION     DAORZF010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38481_length_1987_cov_376.943064"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="PK015_06980"
     CDS             complement(<1..203)
                     /locus_tag="PK015_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34231.1"
                     /translation="MNYILDKEEGMKTNNPSWTPTLTLAKLFEEQKQFYEALAIYELI
                     YQTDKSPAIQEHIEEMHSRIVNNP"
     gene            complement(404..715)
                     /locus_tag="PK015_06985"
     CDS             complement(404..715)
                     /locus_tag="PK015_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34232.1"
                     /translation="MNPEINLVFTKHNDNPVTIIWDRGDKISFNRCYLAWELKLNKIY
                     QQKIPESVTMRAYIDIFHNALDVCNLALNPYAGKALISPTLDLVINYGNPLITRALIW
                     N"
     gene            complement(996..1475)
                     /locus_tag="PK015_06990"
     CDS             complement(996..1475)
                     /locus_tag="PK015_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL34233.1"
                     /translation="MKVYYKNLIKAYSGEFDDLIYCYNPRLKCYYARSHSPRRKTAQN
                     QLYADIAKNMKRLSPSPGYRQDLTNYINAYNSMKEYREHPLNNWYNAFTKLMWNMAKT
                     YSLNGIPLTENSTLIDLTTLTREDIIENNLSCRTVKQAIEAGLLPEVPGYEFYNNEI"
BASE COUNT          596 a          327 c          374 g          690 t
ORIGIN      
        1 ggattattca caatgcgaga atgcatctct tctatatgtt cctgtatagc aggagatttg
       61 tctgtttgat agataagttc ataaattgct aacgcttcat aaaactgttt ttgctcctca
      121 aaaagttttg ccagagttag cgtgggtgtc caacttggat tattggtctt cataccttct
      181 tccttatcta aaatatagtt cataaaggtt aaactgccaa tttatgtcaa tgaaaacctt
      241 atcctgtatt ccagaataag agctttaatt gaaatatttt aaactgagac tggctatttt
      301 atatttttga attcaagtga taaaagctga gaacaggact tgtaatccag gaaatcagct
      361 ttccagatta agaaaattgg ggtatcttat acaattctga gtattaattc cagattaaag
      421 ctctggttat caggggatta ccataattga taactaaatc taaggtggga cttatcaatg
      481 cttttcctgc atatggatta agtgctaagt tacaaacatc cagagcatta tggaaaatat
      541 caatataagc cctcatagta acagattccg gtattttttg ctggtatatt ttatttaact
      601 tcagttccca tgccagataa cagcgattaa aacttatctt atcacctcta tcccatatta
      661 tagttaccgg gttatcatta tgtttagtga agaccaagtt aatttcagga ttcacaggta
      721 aaaggtttga tatacctgtt tcaggtaagt cttcttttgt tccttttgtc ggtaaattaa
      781 agaatagaat ccgttcaaaa agaaaaaacc agaagggtat attgcctggt cgtttgaacc
      841 tatttaaatt aattaagcaa ggattcgttt ttcaaaatca agctcttccc ctgattcagg
      901 aatagctatg attttgactt ctggactgta aggtatagct gagtaattaa aatttggtct
      961 taatgctatg gattttatgg gccattggga gtcttttata tttcattatt atagaattca
     1021 tatccgggaa cttctggtaa caaccccgct tcaatagctt gtttaacggt tctgcaggaa
     1081 aggttatttt ctataatgtc ttcacgggtc agagtggtaa ggtcaataag ggtgctattt
     1141 tccgtgagag gaatcccatt aagagagtag gtttttgcca tattccacat caatttagtg
     1201 aaggcattat accagttatt gagaggatgt tcccgatatt ctttcataga gttataagca
     1261 ttgatatagt tagtgaggtc ctgtcggtat cctggagagg gagataatct cttcatattt
     1321 tttgcgatat cggcatagag ctggttttgt gctgtcttgc gccggggtga atgagaacga
     1381 gcatagtagc atttcaagcg tggattgtaa caataaatca ggtcatcaaa ttcaccgcta
     1441 tacgctttaa tcaggttttt gtaatatact ttcattttgc cctcctataa gttataaaag
     1501 tgtaaattat attgagttgg tggtgagctt atgtccactt tcggacataa gctggacata
     1561 agtcggacat gagctggaca tcagctctcc atttaaagca aaaaaattaa tctctccata
     1621 aatataggga tgacatttaa tggtgtgtgg attttaatgg tgtgcagatg tcctcatctg
     1681 caccaagcgg cagcaattat aagcatatcg gaaagaaatt gaaagcggta taccttatac
     1741 atgtattttc attcataatt tcataagtta atcacgcaac tatggtctta gcgataagtc
     1801 ataatcttga ggacatgatg caaaggagga cctttgcata ccattgcctt tgcataccat
     1861 tacttttgca taccattgcc ttcgcatacc tttattaatg cgaaatgtat tcatctaata
     1921 taggtaccag gattttctta gctttgtttt atttttctta actttttatg atgaagtaaa
     1981 aactcct
//
LOCUS       DAORZF010000084         1953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_39371_length_1953_cov_374.309800, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000084 DAORZF010000000
VERSION     DAORZF010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39371_length_1953_cov_374.309800"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..1095)
                     /gene="arsB"
                     /locus_tag="PK015_06995"
     CDS             complement(46..1095)
                     /gene="arsB"
                     /locus_tag="PK015_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HOL34234.1"
                     /translation="MKANQKKDIGIFEKYLTLWVIICMIAGVLIGKFLPGIPAFLSKF
                     EYAQVSIPIAVLIWLMIYPMMMKVDFNSIKNIRKNPQGLYVTWVANWLIKPFTMYGIA
                     WFFFYIVFQKIIPAELARDYLAGAILLGAAPCTAMVFVWSHLTKGDPTYTVIQVATND
                     LIILVAFTPIVTFLLGISGIKIPWNTLFLSVLLFVVIPLLSGYLTRKRVIKKRGLEYF
                     QQIFIPKFNPLTMIGLLLTLVIIFSFQGKVILSNPLHILLIAIPLIIQTFFIFSLTYL
                     VSWKLHLPHNISAPASMIGASNFFELAVAVAISLFGTSSPVVLATVVGVLVEVPVMLT
                     LVNIANKTRQWFYSD"
     gene            complement(1125..1412)
                     /locus_tag="PK015_07000"
     CDS             complement(1125..1412)
                     /locus_tag="PK015_07000"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOL34235.1"
                     /translation="MDYKENSLLFKALSDPVRLRILDLLSSGELCACELLKKLTISQP
                     TLSHHMKILMRCGLVQAHKKGTWIHYKINPEKVNYLHQIIDGIIQSKNKTF"
BASE COUNT          699 a          310 c          308 g          636 t
ORIGIN      
        1 gacaaatctt gcattaatta atgattaaag gttatcatca ttatattaat cagaataaaa
       61 ccattgcctg gttttattag caatattgac cagagttagc attactggca cttctacgag
      121 aacaccaact actgtggcta gaactacagg agaagaggtt ccaaatagcg agattgcaac
      181 tgctactgcc aattcaaaga aattggacgc tccaatcata cttgcaggag cagaaatatt
      241 atgaggaaga tgtaatttcc aggataccag ataggtaagg gaaaatataa aaaaggtctg
      301 tattatcagt ggtatagcga ttaatagaat gtgcaaagga ttagaaagta tcacttttcc
      361 ttggaatgaa aatataatta ctaaagtaag taatagtcct atcatcgtta aagggttaaa
      421 tttaggaata aagatttgtt gaaaatattc caaccctcgc ttttttatta ccctttttcg
      481 ggttaaataa ccacttaata aaggaatcac tacaaacaat aatactgata gaaataaagt
      541 attccagggg atttttatcc cgcttatacc caataagaaa gtcacaatag gagtgaaagc
      601 tactaaaata atcaaatcat tggttgctac ttgaattaca gtgtaagtag gatcaccctt
      661 ggttaaatga ctccacacaa aaaccattgc agtgcagggt gctgctccta aaagaatagc
      721 tcctgcaaga taatcacgtg ctaattcagc aggtataatc ttctggaaga caatatagaa
      781 aaaaaaccaa gcaattccat acattgtgaa aggttttatc agccaattag caacccatgt
      841 tacatataga ccctgaggat ttttccgaat atttttgatg ctgttaaaat ctactttcat
      901 catcatagga tatatcatta accagatgag aacggctata ggaattgaaa cctgggcata
      961 ttcaaattta cttagaaatg ctggaatacc gggaagaaat ttgcctataa gtactcctgc
     1021 aatcatacaa ataattaccc acagcgttaa atatttctca aaaataccaa tatctttttt
     1081 ctggtttgct ttcataggta ttctctttta tactttttga tatgttaaaa ggtcttattc
     1141 tttgattgta ttatcccatc tataatttga tgcagataat ttactttttc tggatttatt
     1201 ttataatgta tccaggtgcc ttttttatga gcttgaacaa gtccacatcg catcaaaatt
     1261 ttcatatgat gagaaagagt aggttgagaa atggtcagct tctttaataa ttcacaagca
     1321 cataattctc cagaagataa caggtctaat attcgtaatc ttactggatc acttaaagct
     1381 ttaaataata gagaattctc tttataatcc atattatttt gaccttctga taaactctat
     1441 tatacaaaat tgatattcat ttatatatgt caatatttat gtttatcaat ataagcataa
     1501 gattattata aagccttact tacgattatt cagattactc cctaacttaa ggacaaattt
     1561 tcataatctc aaggatggca aaaatgagaa tgatttcaat ttctatgtct ttgggtcaaa
     1621 tatttaggga aactgtagta tatctaattg tctaaaattg agttataaat gtccttattt
     1681 actaagcttt gaagaatctt taaaaaccaa gttattaaaa aattcaaagt ttagtgtcca
     1741 aaaaatataa actgtattat gtatcaatac gttagcttta gaaatgcgaa acttcggcta
     1801 agtttaaatt agagttaaag agtagtttta ttccttatcg ctaacttaaa aaaatgtaac
     1861 tatcatataa agaaagaaag gcattacggc tttgcaagac gaggacgtct tgcaaccaat
     1921 ggtggaacga tgtcctcatc gtttcaagct ccg
//
LOCUS       DAORZF010000085         1589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera thermopropionivorans
            isolate AMR_MDS_1942 NODE_52816_length_1589_cov_379.655150, whole
            genome shotgun sequence.
ACCESSION   DAORZF010000085 DAORZF010000000
VERSION     DAORZF010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863471
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera thermopropionivorans (wastewater
            metagenome)
  ORGANISM  Candidatus Syntrophosphaera thermopropionivorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,401
            CDSs (total)                      :: 1,353
            Genes (coding)                    :: 1,351
            CDSs (with protein)               :: 1,351
            Genes (RNA)                       :: 48
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Candidatus Syntrophosphaera
                     thermopropionivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52816_length_1589_cov_379.655150"
                     /isolate="AMR_MDS_1942"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2593015"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1589)
                     /gene="htpG"
                     /locus_tag="PK015_07005"
     CDS             complement(<1..>1589)
                     /gene="htpG"
                     /locus_tag="PK015_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOL34236.1"
                     /translation="QNSIIGHFGLGFYSTFMVAKKVTIDSLSYLPDSVPAYWECDGST
                     DYIMKEGKRKNVGTTVTVYLNEDSLIYADEGKIQEILEKYCNFTPWPIMFKGKQVNMK
                     EALWNRRPQEITEEEYKEFYKKLFHDWEDPVFWIHLNVDFPFNLKGILYFPKLRNEPD
                     FFKGEVKLYCNNVFVADNLEELIPEFLLLLKGGIDIPDIPLNVSRSFLQNDAQVQKIS
                     RYIVKKVADRFNEIFKEDRKKYEELWKDINSFIKFGLIKDDDFFEAMKDIILFKSASG
                     DYVTLEEYKTRNKSEGEKTRIWYASSEDTQVTYLKMMKEQGIEVIFQDSPLDNHLYQH
                     LEYKFNNIEFVRVDSELNDLLINKDKKELVDLENRTDSEKLTEIFYRALGQNVEASFS
                     KESYGDFLKKHPQAANILAPYIKNEGDRIIINLYDMQPAVREQLGKSTLDDLFDHVYT
                     ELKVEVKSLKTPEIPSMIVFNEFMRRWHDMESFVHNANPGMLKPHTLVVNQENPIIKK
                     ILELDAAGKKEEVKTLCNYVH"
BASE COUNT          483 a          322 c          241 g          543 t
ORIGIN      
        1 tgatgcacat aattgcataa agttttcact tcttctttct ttcctgcagc atccagttca
       61 agaattttct ttataatagg attttcctga tttaccacta aggtatgcgg tttcagcata
      121 cctggattgg cattatgaac gaaggattcc atatcatgcc agcgacgcat aaactcattg
      181 aacacaatca tagagggaat ttctggagtt ttcaggcttt tcacttccac tttcagttcg
      241 gtgtaaacat ggtcaaaaag gtcatccaga gtagatttcc ctaattgttc acggacagca
      301 ggttgcatat cataaagatt aataatgatg cggtctccct cattttttat atatggagca
      361 agaatattag ctgcttgagg atgttttttc aagaaatcac cataactttc tttactgaaa
      421 gaagcttcca cattttgtcc gagagcacga tagaatatct ccgtcagttt ttctgaatca
      481 gtccgatttt ccaggtctac cagttctttt ttatctttat taatcagtaa atcatttagc
      541 tcactatcca ctctcacaaa ctcaatatta ttaaatttat attccagatg ttgatagaga
      601 tgattatcta aaggagaatc ttggaagata acttcaattc cttgttcctt catcattttt
      661 agataggtaa cctgagtgtc ttcacttgaa gcataccaga ttctggtttt ttcaccttcg
      721 ctcttatttc ttgtcttata ttcttccagc gttacataat ctcctgaagc ggatttaaat
      781 aaaatgatat ctttcatcgc ttcaaagaaa tcatcatctt taattaaacc gaacttgata
      841 aaggaattaa tatccttcca gagttcttca tacttcttac ggtcttcttt aaagatttca
      901 ttaaaacggt cagctacttt tttaacaatg tatctgctaa ttttctgcac ttgcgcatcg
      961 ttctgcagaa aactacggga aacattaaga ggaatatcag gaatatcaat gccacctttc
     1021 agtaagagca gaaattcggg aataagttct tccaaattat ctgcaacaaa aacattatta
     1081 cagtagagct tgacctcacc cttaaagaaa tctggttcat tcctcaactt gggaaaatat
     1141 agtatacctt ttaaattgaa gggaaaatct acatttagat gaatccagaa aactggatct
     1201 tcccaatcat gaaataactt cttataaaat tccttatatt cttcctctgt aatctcttgc
     1261 ggtctgcgat tccagagggc ttccttcata ttgacctgtt tacctttaaa cataataggc
     1321 cagggagtaa aattacaata cttttccagt atttcttgaa tcttgccctc gtctgcataa
     1381 atgagagaat cttcattcag atagactgta accgttgttc ctacattctt acgtttccct
     1441 tctttcataa tataatctgt gctaccatca cattcccagt aagcaggaac actatccggc
     1501 aaataggaaa gtgaatctat agtaaccttt ttagctacca taaaggtgga ataaaaaccc
     1561 agaccaaaat gcccaattat gctgttttg
//
